LOCUS       DASWAV010000001         1591 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U2665
            k141_520454, whole genome shotgun sequence.
ACCESSION   DASWAV010000001 DASWAV010000000
VERSION     DASWAV010000001.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960316
            Sequence Read Archive: SRR10260007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 1591)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1591)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:47:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,929
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1591
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_520454"
                     /isolate="SMAG_U2665"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.36444 N 132.31889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1501)
                     /locus_tag="VF958_00005"
     CDS             complement(<1..1501)
                     /locus_tag="VF958_00005"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEX9147515.1"
                     /translation="MIAVGTRLGPYEILSPLGAGGMGEVYRAKDPRLGREVAVKVLPA
                     SFSTDADRLRRFEQEARAAGLLNHPNITSVFDIGSHDGAPYVVTELLEGETLRSRLSV
                     GPLAPRKALDYAIQVARGLAAAHEKGIVHRDLKPENLFVTKDGRVKILDFGLAKLKQA
                     EPGSEGETNLPTGTAGTEPGVVLGTMGYMSPEQVRGKPADARSDIFSFGAILHEMLSG
                     RRAFHGDTAADTITAILTKEPPDLSQTNSDVHPGLDRIIRHALEKNPEERFQSARDLA
                     FDLEALSGISSTSAITPSAVARVRRRSLIGPLAIAAGVAAALALGFVLGRQTGSSAPP
                     TFHQLTFRRGTIGTARFGPDGQTIVYSAAWEGKPLEPFVARSETPDSRPFGILGAEIL
                     GISKPGEIALALGSHRALPFTQAGTLARATLAGGAAPREVLEEVQFADWSPDGRELAV
                     VRIVGAKTRLEYPIGKVLLETDGWVGHPRISPKGDRIAFLEHEFRGDNGG"
BASE COUNT          238 a          552 c          542 g          259 t
ORIGIN      
        1 tgccgccgtt gtcgccgcga aactcgtgct cgagaaaggc gatccggtcg cctttcgggg
       61 atatccgggg gtgcccgacc cacccgtccg tctcgagaag gaccttcccg atcgggtact
      121 cgaggcgtgt cttcgctccg acgatgcgca cgaccgcgag ctcccgaccg tccgggctcc
      181 agtcggcgaa ctgcacctct tcgaggactt cgcggggcgc cgcgcctccg gcgagcgtcg
      241 cacgggcgag ggtgcccgcc tgcgtaaagg gcaacgcccg gtggctgccg agtgcgagag
      301 cgatttctcc gggcttcgag ataccgagga tctccgcccc gaggatcccg aacggcctcg
      361 agtccggtgt ctccgagcgg gcgacgaacg gctcgagagg tttcccttcc cacgcggcgc
      421 tgtaaacgat cgtctggccg tccgggccga atcgggcggt cccgatcgtc ccgcggcgga
      481 acgtgagctg gtggaaggtc ggcggagcgc tgcttccggt ctggcgcccg aggacgaatc
      541 cgagcgccag tgccgcggcc acgccggcgg cgatcgcgag gggcccgatc aggctgcggc
      601 gacggactcg cgcgaccgcc gaaggagtga tcgcgctcgt cgaggagatc cccgacagcg
      661 cctcaaggtc gaacgcgagg tcgcgcgcgg attggaaccg ctcttccgga ttcttctcga
      721 gcgcgtgccg gatgatccgg tcgagccccg ggtggacgtc gctgttggtc tgcgagaggt
      781 ccggcggttc cttcgtcagg atcgccgtaa tcgtgtcggc cgcggtgtca ccatggaatg
      841 cgcgacggcc cgacagcatc tcgtggagga tggcgccgaa cgagaagata tcgcttcgcg
      901 cgtcggcggg cttcccgcgc acctgctcgg gcgacatgta ccccatcgtg ccgagcacga
      961 cgcccggctc ggttcccgca gtgccggtcg gaaggttggt ctctccttcg cttcccggct
     1021 ccgcctgttt gagcttcgcg agaccgaagt cgaggatctt cacgcgtccg tctttcgtga
     1081 cgaacaggtt ctcgggcttc agatcccggt ggacgatccc cttctcgtgc gccgcggcca
     1141 gcccgcgcgc gacctggatc gcatagtcga gagctttcct cggcgcgagc ggccccaccg
     1201 agagccggct ccggagcgtc tccccctcga gaagctccgt gacgacgtag ggcgcgccgt
     1261 cgtgcgaccc gatgtcgaag accgacgtga tgttcggatg attcaggagc cccgccgccc
     1321 gggcctcctg ctcgaagcgg cgcagccggt ccgcgtccgt cgaaaacgac gccggcagga
     1381 ccttgaccgc gacttcgcgt cccagccgcg gatccttcgc gcgatacacc tcccccattc
     1441 cgcccgcgcc gagcggcgag aggatctcgt acggacccaa ccgcgttcca acggcgatca
     1501 cgagtcggta atcaccatca cgaatcctac ggcggcaggc cgacgcggcg aagcagggat
     1561 ggcgtactcg ccggctttgc ggacggacag g
//
LOCUS       DASWAV010000002         2685 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U2665
            k141_680633, whole genome shotgun sequence.
ACCESSION   DASWAV010000002 DASWAV010000000
VERSION     DASWAV010000002.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960316
            Sequence Read Archive: SRR10260007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2685)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2685)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:47:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,929
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2685
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_680633"
                     /isolate="SMAG_U2665"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.36444 N 132.31889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..362)
                     /locus_tag="VF958_00010"
     CDS             complement(<1..362)
                     /locus_tag="VF958_00010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9147516.1"
                     /translation="MLGRIDQDLIRAVNVAVREAARKLEAPACQMVFSAFQDAAGHTI
                     RENLHAKRQSEVLYLKWLIFVNGSEDHFCLDSQIIAGTNPGDRFVRLCGSRFKSIVRS
                     DTGFAAALIIHEELHSLGL"
     gene            619..1272
                     /locus_tag="VF958_00015"
     CDS             619..1272
                     /locus_tag="VF958_00015"
                     /inference="COORDINATES: protein motif:HMM:NF012698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HEX9147517.1"
                     /translation="MRVRFGDCTLDSDTRELSRGGEPVHLEPKAYRLLELLLAVCPKA
                     LAKRDLQEDLWPQTYVSERSLARLVADLRTAVGDRASQPRLIRTVHGFGYAFCGEVAG
                     ADRRRGKRSSDFHCRLSWGDRDIALVEGENVLGRDPDVAVWIDLNSVSRHHARVVVTD
                     GAAHLEDLGSKNGTLVNGERISSPRQLSNGDRIKVGAASLVYRCSRRLGTTEAETGS"
     gene            1269..>2685
                     /locus_tag="VF958_00020"
     CDS             1269..>2685
                     /locus_tag="VF958_00020"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HEX9147518.1"
                     /translation="MTSKPGATAARRLGPYEFLGRLGAGGMGEVYRARDTRLGREVAI
                     KVLPPDFSSDADRLRRFEKEAQAASSLNHPNIVTIYEVERIDSTSFIVMELVEGKTLR
                     ELVAQGAMPVRRLLGIAVQIADGLARAHSAGIVHRDLKPENVMVTKEGLVKILDFGLA
                     KLAHPDLESGQEAESPTVSAGTKPGVVMGTVGYMSPEQASGHPVDFRSDQFSFGAVLY
                     EMATGKGPFRRATAAQTLAAIIQDEPDPVAAVNPKVPAPLRWIIERCLAKDPRERYAS
                     TEDLARDLGGVRDHLSEVSSSGEAVATLARPRRRLLWLLLAGSAALVVIALAAGYLRR
                     APRQSLPIRSSLTFPEKMVLQHLALSPDGRRLAFTAQPSTCAGCSAATSTGGTLWMRA
                     LDGSAPQPIAAGDDVSLPFWSPDNRYLAFFSDGKLKKIDAAGGAIQTICDVEWGGGGT
                     WGLDGTIVFASGVTSALYKVFP"
BASE COUNT          457 a          863 c          890 g          475 t
ORIGIN      
        1 aggccgagcg agtgtagctc ctcgtggatg atcaaggcgg cggcaaagcc ggtgtcgctt
       61 cggacgatcg acttgaagcg cgatccgcag agcctcacga accggtctcc gggattcgtg
      121 cccgcgatga tctggctgtc caggcagaag tggtcttccg agccgttcac gaagatcagc
      181 cacttcaggt aaaggacctc gctctgacgc ttcgcgtgca ggttttcgcg aatggtatgt
      241 ccggccgcgt cctggaacgc ggaaaacacc atctggcacg cgggcgcctc gagcttccgc
      301 gcagcctccc ggacggccac gttgacggcg cggatcagat cctggtcgat ccttcccagc
      361 acggagattc ttggctcttc tcggacttcg gaggtctctg ccaagacggt cgagtgtaag
      421 aggaagatcg agaagagggc ctcgaaacta aggaccctgg ccgccgtgtc gcgtccccgc
      481 atgagccgct ccttccggag cgcccgggcg ccgccctgcc ggcggcgtcg ttttggcgcg
      541 acacttagga ttaggattca cggagctcga atctccaggt ccggtttcgg gtttgttccc
      601 cttttgttcg gggcgacagt gcgagttcga ttcggcgatt gcacgctcga ctccgacacg
      661 cgggagctct cgcgcggcgg cgagcccgtg catctcgaac cgaaggctta ccggctactt
      721 gagctgctac tcgccgtctg cccgaaggcc cttgcgaagc gcgatctcca ggaggacctc
      781 tggccgcaga cgtacgtttc ggagcggagc ctcgcgaggc tggtcgcaga ccttcgaacg
      841 gcagtgggag accgagcgtc gcaaccgcgc ctcatccgca ctgtgcacgg cttcggctac
      901 gcgttctgcg gcgaggtcgc cggggcggat cgtcggcggg ggaagcgttc gagcgacttt
      961 cactgccggc tctcctgggg agaccgcgac atcgcgctcg tcgaaggcga gaacgttctc
     1021 ggacgcgatc ccgacgtcgc cgtctggatc gatctcaaca gcgtgtcccg ccatcacgcc
     1081 cgcgtcgtcg tgaccgacgg agccgctcat ctcgaagacc tgggaagcaa gaacggcacg
     1141 ctcgtcaacg gcgagcgcat cagttccccg agacaactct ccaacggcga ccgcatcaag
     1201 gtcggcgcgg cctcgcttgt ctaccgctgc tctcggagac tgggcacgac cgaggcggag
     1261 acgggatctt gacctccaag ccgggcgcca ccgcagcacg ccggctcgga ccttacgaat
     1321 ttctcggtcg tctcggtgcc ggcggaatgg gggaggtgta tcgggctcgc gacacgcggc
     1381 tgggccgcga ggtcgcgatc aaggttctgc cgccagactt ctcatcggac gcggatcgtc
     1441 tgaggcgatt cgagaaggag gcccaggcgg catcgtctct caaccacccc aacatcgtga
     1501 cgatctacga ggtcgagcgg atcgactcga cgtccttcat cgtcatggag ctggtcgaag
     1561 ggaagacgct gcgggagctc gtcgcgcagg gcgcaatgcc ggttcgccgg cttctgggga
     1621 tcgcggtgca gatcgcggac ggtctcgcgc gcgcgcactc ggcagggatc gtccaccggg
     1681 atctgaaacc cgagaacgtg atggtcacga aagagggact cgtcaagatc ctcgacttcg
     1741 ggttggcgaa actggcgcat ccggacctgg agagtggaca ggaagcggag tctccgactg
     1801 tgtccgccgg tacgaagccc ggcgtcgtga tgggaaccgt cggctacatg tctcccgaac
     1861 aggcgtcggg gcatccggtg gactttcgtt ccgatcagtt ctcattcggt gcggtgctgt
     1921 acgagatggc gacaggcaag gggccattcc gacgcgcgac cgcggcacag actctcgcgg
     1981 cgatcatcca ggacgagccc gacccggtcg ccgcagtcaa ccccaaagtc ccggcgccgc
     2041 tccgctggat catcgagcgg tgcctcgcca aggaccctcg ggaacggtat gcctcgacgg
     2101 aggatctggc gcgagatctc ggtggtgtgc gggaccacct gtccgaggtc agcagctcgg
     2161 gagaagcagt cgctacgctg gcgaggccaa gaaggcgcct cctttggctg ctcctcgcag
     2221 gctccgccgc actcgtcgtc atcgcgctgg ccgccggcta tctccggcgc gccccccgcc
     2281 agtcactacc gatccggtcc tcgctgacct ttcccgagaa aatggttctg cagcaccttg
     2341 cactgtcccc ggacggcagg cgcctcgcct tcacggccca gccgtcgacg tgtgccgggt
     2401 gctcggccgc gacgagcacg ggcggtactc tttggatgcg ggccctcgac ggaagtgcgc
     2461 cgcagccgat cgcggccggt gacgacgtca gcttgccgtt ctggtcaccg gacaaccgtt
     2521 acctggcctt cttcagcgac gggaagctga aaaaaatcga cgccgccgga ggggcaatcc
     2581 agaccatctg tgatgtggag tgggggggcg gcgggacatg gggacttgac ggaaccatcg
     2641 tcttcgcgtc gggggttaca tccgctctgt acaaagtttt tcccg
//
LOCUS       DASWAV010000003         1850 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U2665
            k141_40275, whole genome shotgun sequence.
ACCESSION   DASWAV010000003 DASWAV010000000
VERSION     DASWAV010000003.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960316
            Sequence Read Archive: SRR10260007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 1850)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1850)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:47:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,929
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1850
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_40275"
                     /isolate="SMAG_U2665"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.36444 N 132.31889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..376)
                     /locus_tag="VF958_00025"
     CDS             complement(<1..376)
                     /locus_tag="VF958_00025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9147519.1"
                     /translation="MAWFEHPSKFPEDGVNLFESEVLHDTKIPDTRHRVVRQRKAADV
                     RLELTAVEREPSSGLQGFEREIGCDQSPTVPEDARRGLTRPAPALHERSLPWEESVRI
                     TLYRATEDPPSDKGRELFESRAR"
     gene            622..1341
                     /locus_tag="VF958_00030"
     CDS             622..1341
                     /locus_tag="VF958_00030"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX9147520.1"
                     /translation="MKLVAVIAAYNERDNVEELTRRLLASLSSSKDLSGEVLYVVEGD
                     DGTREVIERLQSELPGIRILYQREATGLADAFRRGFAALPADCDFVLTMDADLNHRPE
                     EIPRLLQMILASGADIVVGSRFVPGARIEGVPIWKRMLSGLGNLGMGIAFDRKVRDKT
                     SGFRIYRAGALRSLRFESSGFAFLPEILLRASAMDMTIVEAPIHFVYRVHGGSKMRLW
                     GTAKSYMDLARRRIAGDLTPH"
     gene            1438..>1850
                     /locus_tag="VF958_00035"
     CDS             1438..>1850
                     /locus_tag="VF958_00035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9147521.1"
                     /translation="MKFIEIRRRWSLLALFGFALLVRLYGLNWDQRHFFHPDERAIAM
                     AIERLSFIPPQFNPHFFAYGSFPLYVTKVVTALLGNVSGWFTGYDGIIYVGRALSAIW
                     GAATVVLLAVFGSRLYGRRAGLLAGGLLAVTVLHV"
BASE COUNT          302 a          578 c          594 g          376 t
ORIGIN      
        1 cgcgggctcg gctttcaaaa agctcacggc ctttgtccga aggtggatcc tcagtcgcgc
       61 ggtacagagt tatacggacc gattcttccc acgggaggga acgttcgtgg agtgcgggtg
      121 cgggacgggt caatcctcga cgcgcgtctt ccggaacggt cggcgactga tcgcagccga
      181 tctctctctc gaagccttgc aggccactcg aaggctcgcg ctctacagcg gtcagctcca
      241 ggcggacatc cgccgccttc cgctggcgga cgactcggtg gcgggtatct ggaatcttgg
      301 tgtcatggag cacttcggat tcgaagaggt tgacaccgtc ctccgggaat ttcgaagggt
      361 gctcaaacca ggccacgcgg cgatcctgtt ctggccgccc gagttcagtt cgtcacgact
      421 ggtgctcgcc ccgatcgaat ggttccttac gcggagacgc ggccgccctt ttcgcttttt
      481 cccgagtgag cccagccggt tgaggtctca aagagaggca cgcgcgcgga tagccggtgc
      541 cggattcgag ccggtggcgg ccgagtttgg gccgcgggac gctttcattc acgtcgtagt
      601 ggttggacga aagcccgaga gatgaagctc gtcgccgtga tcgccgccta caacgagcgc
      661 gacaacgtcg aggaattgac ccgccggcta ctcgcctctc tttcgagctc gaaagatctc
      721 tccggggagg tcctgtacgt cgtcgaagga gacgacggga cccgggaggt catcgaacgg
      781 ctacagagcg agcttccagg cattcggatc ctctaccagc gggaagcgac gggcctggcc
      841 gatgccttcc ggcgcggctt tgcggcgttg cccgcggatt gcgacttcgt cctgacgatg
      901 gacgccgatc tgaatcaccg gcccgaggag attccgcgcc tgcttcagat gatcctcgcg
      961 agcggagccg atatcgtcgt cggctcccga ttcgtcccgg gagcgcggat cgagggggtg
     1021 ccaatctgga agaggatgct gagcggcctg gggaatctcg gaatgggcat cgccttcgac
     1081 cggaaagttc gcgacaagac gtccggcttc cgcatttatc gcgccggcgc gctgcgatca
     1141 cttcgcttcg agagctccgg gttcgccttc cttcccgaaa tactgcttcg cgcgagcgcc
     1201 atggacatga cgatcgtcga agctccgatt catttcgtct accgggtgca cggcggctcg
     1261 aaaatgcgtc tgtggggaac cgcgaaaagc tacatggatc tcgcgagacg ccgcatcgcc
     1321 ggagacctca ctccccactg agcgtgaact ctcgaggtcg gaactccgct cggggcggag
     1381 gacgttcccg ggcttgcggg cctgttggtt tctggtagcc tccgcgccga caaaccgatg
     1441 aaattcatcg agatccgccg gcgctggagc ctcctggcgc tcttcggctt cgcactgctc
     1501 gtgaggctgt acgggctcaa ctgggatcag cgtcacttct ttcatcccga cgagcgggcg
     1561 atcgcgatgg cgatcgaacg gctgtctttc ataccccccc agttcaaccc gcacttcttt
     1621 gcgtatggat cgttccctct ctacgtgacg aaggtcgtca ccgccctcct tggaaacgtg
     1681 agcggatggt tcacgggtta cgacggcatc atctatgtgg gccgggcgct ttcggcgatc
     1741 tggggggccg cgaccgtcgt tctcctcgcg gtgttcggct cgcggctcta cgggcgccgc
     1801 gccgggcttc tcgcgggagg tctgctggcg gtcaccgtcc tccacgtcca
//
LOCUS       DASWAV010000004         5531 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U2665
            k141_40366, whole genome shotgun sequence.
ACCESSION   DASWAV010000004 DASWAV010000000
VERSION     DASWAV010000004.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960316
            Sequence Read Archive: SRR10260007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 5531)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5531)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:47:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,929
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5531
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_40366"
                     /isolate="SMAG_U2665"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.36444 N 132.31889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..294
                     /locus_tag="VF958_00040"
     CDS             <1..294
                     /locus_tag="VF958_00040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012099157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-methyl-5-thioribose-1-phosphate isomerase"
                     /protein_id="HEX9147522.1"
                     /translation="PFYVAAPTTTIDLACPDGTAIPIEERAAAEVLSFAGHSIAPAGV
                     GARHPAFDVTPARYIRAIVTDEGICRPPYSDSLAAAVRAATDRRSRQSVVGNR"
     gene            329..619
                     /locus_tag="VF958_00045"
     CDS             329..619
                     /locus_tag="VF958_00045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006592763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008219 - cell death [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system
                     prevent-host-death family antitoxin"
                     /protein_id="HEX9147523.1"
                     /translation="MKNARISELRDRLSEYLARVRKGETVIVYDRDTPIARIDPIAPT
                     ADNTPEWVLEAYRRGIVTPAKVRDGKRIPPPMKVKEPLGLLEALLEERRTGR"
     gene            616..1035
                     /locus_tag="VF958_00050"
     CDS             616..1035
                     /locus_tag="VF958_00050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HEX9147524.1"
                     /translation="MRFWDSSALIPLLLDEGRSKDVWDLYEDDQQQTVWCLTEVELAS
                     AVARKVREGMNVVREKAARSQLEVLRERWDEITSLQNVRTRALRLLGTHALRSADALQ
                     LAAALVFCDERTESLPFVCLDDRLADAARKERFPVLP"
     gene            1049..2092
                     /gene="tsaD"
                     /locus_tag="VF958_00055"
     CDS             1049..2092
                     /gene="tsaD"
                     /locus_tag="VF958_00055"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005010263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HEX9147525.1"
                     /translation="MLVLGIETSCDETSVAVLDGEGRVRSNVVSSQLAEHAPYGGVVP
                     EIAARAHLENLPVTFERALAEAGTDVSGIDLVAATAGPGLIGALLVGLSAAKALAFAR
                     GVPLVPVNHLEGHLYSAFLRGHGEPALPVELPFHGLVVSGGHTELVRVDGSGITPLAR
                     TRDDAAGEAFDKVARRAGLGYPGGPVVDRIASAGDGSRHPLPVGRPGDGSNDFSFSGL
                     KTAMLREFQRRGVDGRPLDPASLDADMTDLLASFQRAIVTALVDRVETVHSDEGIRAL
                     AVSGGVAANSELRRTLLTWGDSRGVPVLLPERAFTTDNAAMIAFAGLLRHQRNGRPAP
                     VAAAARSRWPLGS"
     gene            2100..3899
                     /gene="lepA"
                     /locus_tag="VF958_00060"
     CDS             2100..3899
                     /gene="lepA"
                     /locus_tag="VF958_00060"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020721028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor 4"
                     /protein_id="HEX9147526.1"
                     /translation="MTDPRLIRNFAIVAHIDHGKTTLSDRILQRCGAVADREMHEQML
                     DDMELEQERGITIKARSVTLRYTARDGLVYVLNLIDTPGHVDFSYEVSRSLAACEGAV
                     LVVDATQGVEAQTLANAYLASHNNLTIVPVINKIDLPSAEPEKVREQIEQVVGIPAHD
                     AVLTSAKQGIGIDDLLERIVRDVPPPKGDPDAPLQALLFDSWYDPYRGVTSLVRVFQG
                     TLSPRKKIRAIGTGLQAEVEEVGIFIPGRLTVTSLSVGEVGFLWANIKDLHTTRIGDT
                     ITEVDRPTAHPLPGFAEAKPMVFAGLFPTDTDDYANLRDAMEKLRLNDASFTFQPENS
                     TALGFGFRCGFLGLLHMEIVQERLEREFDLELITTAPGVNYRVTTTKGEVVEIVNPNH
                     LPPPTTVERVEEPWISATIITRDDVLGGLLKLCEERRGVQKSLEYLSANRVVLSYDFP
                     LNEVVLDFYDKLKSISRGYASFDYEFAGYREGDLVKLDILVNGEAVDALSIIVHREES
                     ASKGRLLAAKMKEIIPRQMFEVAIQAAIGSKIIARETVPAMRKNVLAKCYGGDITRKK
                     KLLEKQKEGKKRMKRVGRVDIPQDAFLAVLKVE"
     gene            3896..4630
                     /gene="lepB"
                     /locus_tag="VF958_00065"
     CDS             3896..4630
                     /gene="lepB"
                     /locus_tag="VF958_00065"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: protein motif:HMM:NF021981.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HEX9147527.1"
                     /translation="MSETQAVADTSRPKHSSAREWYESLLVAGIFVLFVRTFVVQTYQ
                     VPTGSMEKTILVGDHLLVNKFAFAPRLEGWAKLFPYRDVHRGDIIVFKKPGDDVNPGN
                     VLVKRAVAGPDQTVLVRDKKLFVDGQPGDVPSVQHSDPEVFPNDPAIPDMARRRDQFG
                     PFRVPPGSWFGMGDNRDNSLDSRYWGAIPRENIFGRPTILYWSYEAEPNSHVWRGPMA
                     KARQLADVAIHFFTRTRWNRMFRLVR"
     gene            4600..5391
                     /gene="lepB"
                     /locus_tag="VF958_00070"
     CDS             4600..5391
                     /gene="lepB"
                     /locus_tag="VF958_00070"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015862576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HEX9147528.1"
                     /translation="MEPDVPARPLTAEAEAESAPAPAFEVETSVQDADLEAAVGARSR
                     VREYLEAVLVAVIFALFARTFLFQAFQVPTSSMEKNILVGDHLIVNKFIFGPHRGGFL
                     SCLLPYRAVERGDVIVFKFPEDPRRDFIKRTVALPGETVEIRDKVIYVEGKRLVEPRI
                     YHADERVWPDDPALPDSSRRRDQSRPFTVPAESYFALGDNRDNSYDSRFWGPVPAGNL
                     KGRAMFVYWSFPLEPSTHRSVRELIEIALNFFRRTRWERTLLPVR"
BASE COUNT          943 a         1824 c         1826 g          938 t
ORIGIN      
        1 ccgttctacg tcgcggcgcc cacgacgacg atcgacctcg cctgccccga cggcacggcg
       61 atcccgatcg aggagcgcgc ggccgccgag gtcctgtcgt tcgccggaca ctcgatcgcg
      121 ccggccggag tgggggcgag acaccccgcg ttcgacgtca cgcccgcccg atacatccgc
      181 gcgatcgtga cggacgaggg gatctgccgt cccccgtact ccgactcgct tgcggccgcc
      241 gttcgcgccg caaccgatcg tcggagccgg caatcggtcg tcggcaaccg gtaaccgagt
      301 ccgatttcgt catggctata atggcgtcat gaagaacgcc agaatctccg agttgcggga
      361 ccggctctcc gagtacctcg cgagggtgcg gaagggcgag accgtgatcg tgtacgaccg
      421 ggacacgccg atcgctcgga tcgacccgat cgcgcccacg gccgacaaca cgccggaatg
      481 ggtactggaa gcgtatcggc gaggaatcgt gacgcccgcg aaagttcgcg acggcaagag
      541 gattccgcct cccatgaagg tcaaggagcc gcttggtctc ctcgaggcgc tcctcgagga
      601 acggcgcaca ggacgatgag attctgggat tcttccgctt tgattcccct gcttctcgac
      661 gaggggcggt cgaaggacgt ctgggacttg tatgaagacg atcagcaaca gacggtgtgg
      721 tgcttgaccg aggtcgagtt ggcttctgcc gttgccagaa aggtccggga gggaatgaac
      781 gtcgtgcgag agaaggcagc tcggtcgcag ctcgaagtct tgagggagcg ctgggacgag
      841 atcacatctc tgcaaaacgt caggacccgt gctctccgcc ttctcggtac gcacgcgctt
      901 cgctccgccg atgcccttca gctcgccgcg gcgctcgtct tctgcgacga gcgcaccgag
      961 agtctcccgt tcgtgtgcct cgacgaccgg ctcgccgacg ccgcccgaaa ggagaggttc
     1021 ccggtcctgc cctgaccgga aattttcgat gctggtcctc ggaatcgaaa cttcctgcga
     1081 cgagacgtcc gtcgccgtgc tcgacggcga ggggcgcgtc cgctcgaacg tcgtctcctc
     1141 gcagctcgcg gagcacgcgc cgtacggcgg cgtcgtgccc gagatcgccg cgcgcgcgca
     1201 cctggagaac ctccccgtca cgttcgaacg ggcgctcgcc gaagcgggca cggacgtctc
     1261 cggcatcgat ctcgtcgcgg cgaccgcggg tccgggattg atcggcgccc tgctcgtcgg
     1321 cctctcggcg gcaaaggcgc tcgcgttcgc gcgcggagtg ccgctcgtgc cggtcaacca
     1381 cctcgaaggg cacctgtact ccgcgttcct gcgcgggcac ggcgaacccg cgctgccggt
     1441 cgagctcccg ttccacgggc tcgtggtctc ggggggacac accgagctcg tgcgcgtcga
     1501 tggtagcggg atcacgccgc tggcgcggac gcgcgacgac gcagccggcg aggctttcga
     1561 caaggttgcg cgacgcgcgg gcctgggcta tccgggcgga ccggtcgtgg accggatcgc
     1621 ctcggccggc gatggaagcc gccatccgct gcccgtcggg cggccggggg acgggtcgaa
     1681 cgacttctcc ttttccgggt tgaaaacggc gatgctccgc gagttccaga ggaggggcgt
     1741 cgacggacgt ccgctcgatc ccgcttctct cgacgccgac atgaccgacc tcctcgcctc
     1801 attccagcgc gcgatcgtga ccgcgctcgt cgaccgcgtc gagaccgtcc acagcgacga
     1861 ggggattcgc gcgctcgcgg tctccggagg tgtcgccgcg aattccgaac tgcggcggac
     1921 tctgctcacc tggggcgact cgcgaggggt cccggtgctc cttcccgagc gcgccttcac
     1981 gacggacaat gcggcgatga tcgcgttcgc gggcctgctg cgccaccagc ggaacggtcg
     2041 gccggctccg gttgcggcgg ccgccagatc ccgctggccg ctgggaagct gaccgaccga
     2101 tgaccgaccc tcgtctgatc cgcaacttcg cgatcgtggc ccacatcgac cacggcaaga
     2161 cgacgctttc cgatcgaatc ctccagcggt gcggcgccgt cgccgaccgt gagatgcacg
     2221 agcagatgct cgacgacatg gagctcgagc aggagcgcgg catcacgatc aaggcgcggt
     2281 ccgtcacgct gcgctacacg gcgcgcgacg ggctcgtcta cgtgctcaac ctgatcgaca
     2341 cgccggggca cgtggatttc tcctacgagg tgtcacgttc gctcgcagcg tgcgagggcg
     2401 cggtgctggt cgtcgacgcg acgcaggggg tcgaagcgca gaccctcgcg aacgcctacc
     2461 tcgcgagcca caacaacctg acgatcgttc cggtcatcaa caagatcgac ctgccttccg
     2521 ccgagcccga gaaggtgcgc gagcagatcg aacaggtcgt cgggatcccc gcgcacgacg
     2581 ccgtgctcac ctccgcgaag cagggcatcg gcatcgacga ccttctggag aggatcgtgc
     2641 gcgacgtgcc gccgccgaag ggggacccgg acgctccgct ccaggcgctg ctcttcgact
     2701 cgtggtacga cccgtatcgc ggagtcacgt cgctcgttcg agtgttccag ggcacactct
     2761 cgccgagaaa aaagatccgg gcgatcggaa cgggcctgca ggcggaggtc gaggaggtcg
     2821 gcatcttcat cccgggccgc ctgaccgtaa cgtcgctgtc cgtcggcgag gtcggcttcc
     2881 tctgggcgaa catcaaggac ctgcacacga ctcggatcgg cgacacgatc accgaggtgg
     2941 accgtccaac cgcgcaccca ctccccggtt tcgcggaggc gaagccgatg gttttcgcgg
     3001 ggctgttccc cacggatacc gacgactacg cgaacctgcg cgacgccatg gaaaagctcc
     3061 gcctgaacga cgcgtcattc acgtttcagc cggagaattc gacggcgctc ggtttcgggt
     3121 tccgatgcgg cttcctcggg ctgctccaca tggagatcgt gcaggaacgg ctggagcgcg
     3181 agttcgacct cgagctgatc acgacggcgc cgggcgtcaa ctaccgcgtg acgacgacga
     3241 agggcgaagt cgtcgagatc gtcaacccga atcacctgcc gcccccgacg acggtcgaac
     3301 gggtcgagga gccctggatc tccgcgacga tcatcactcg cgacgacgtc ctcggcgggc
     3361 tgttgaagct ctgcgaggag cggcgcggag tccagaagtc gctcgagtac ctctccgcga
     3421 accgcgtggt cctctcgtac gacttccctc tgaacgaggt cgtgctcgat ttctacgaca
     3481 agctgaagtc gatttcccgg ggatacgctt cgttcgacta cgagttcgcc ggataccggg
     3541 agggggacct ggtcaagctg gacatcctcg tcaacggtga ggcggtggat gccctctcga
     3601 tcatcgtcca tcgggaagaa tcggcctcca aggggaggtt gctcgctgcg aagatgaaag
     3661 agatcatccc ccgccagatg ttcgaggtgg cgatccaggc cgccatcggg tcgaaaatca
     3721 tcgcgcgcga gaccgttccg gcgatgcgca agaacgtcct cgccaagtgc tacggcgggg
     3781 acatcactcg caagaaaaag ctcctcgaga agcagaagga aggaaagaag cggatgaaga
     3841 gagtgggccg tgtcgacatt ccgcaggacg cgttcctggc ggttctgaag gtcgaatgag
     3901 cgagacgcag gccgtcgcag acaccagccg gccgaagcac tcgtcggcgc gggagtggta
     3961 cgagtctctc ctcgtcgccg ggatcttcgt tcttttcgtg aggacgttcg tcgtccagac
     4021 ttaccaggtc ccgacgggct cgatggagaa gacgattctc gtcggcgacc acctgctcgt
     4081 gaacaagttc gcctttgcgc cgcgcctgga aggatgggcg aagctctttc cctaccgcga
     4141 cgtgcaccgc ggcgacatca tcgttttcaa gaaaccgggg gacgacgtca accccgggaa
     4201 cgttctggtg aagcgggcag tggccggccc ggaccagacg gtgctcgtgc gcgacaagaa
     4261 gctcttcgtc gacggacaac ccggtgacgt cccttccgtc cagcacagcg accccgaggt
     4321 ctttccgaac gatccggcga ttcccgacat ggcgcgccgc cgggaccagt tcgggccgtt
     4381 tcgcgtgccg ccgggctcct ggttcggaat gggagacaac cgcgacaact ctctcgacag
     4441 ccgttactgg ggggcgatcc cgcgggagaa catcttcggc cggcccacga tcctctactg
     4501 gtcgtacgag gcggagccaa actcccacgt ctggcgcgga ccgatggcga aggctcgcca
     4561 gctcgccgac gtcgcgatcc acttcttcac ccggacccga tggaaccgga tgttccggct
     4621 cgtccgctga cggcggaggc cgaagcggag agcgctccgg cgccggcctt cgaggtcgag
     4681 acgtcggtgc aggatgccga cctcgaagcg gcagtcggag ctcgttcccg cgtgagggag
     4741 tacctcgaag ctgttctcgt ggccgtgatc ttcgcgctct tcgcgcggac gtttctcttc
     4801 caggcgtttc aagtgccgac gtcctcgatg gagaagaaca tcctcgtcgg cgatcacctg
     4861 atcgtgaaca agttcatctt cggccctcac cgaggcggct ttctctcgtg ccttctcccc
     4921 tatcgggccg tcgagcgcgg cgatgtcatc gtcttcaagt tccctgagga tccgcggcgg
     4981 gacttcatca agagaacggt cgcactgccg ggcgagacgg tcgagatccg ggacaaggtg
     5041 atctacgtgg agggaaagcg actcgtcgag ccacggatct accacgcgga cgagcgcgta
     5101 tggcccgacg accccgcgct gcccgactcg tcccgccgcc gagaccagtc gcggcccttc
     5161 accgtgccgg ccgagtcgta ctttgcgctc ggggacaacc gtgacaactc ctacgacagc
     5221 cggttctggg gtccggttcc ggccgggaac ctgaaggggc gagcgatgtt cgtctactgg
     5281 tcgttcccgc tggagccgtc gactcaccgc tccgtgcgcg agctgatcga aatcgcactc
     5341 aatttcttcc gccggacgcg atgggagcgt accctgctgc cggtgcggtg aacctccgga
     5401 atccgggtta agattttggt cgacttcagg gaggatcaat gagacgcaga gggccgcgtt
     5461 cgcgcggcgc ggggaacatc ggctgcctgc tctggctggt cgtcgtggtt gcggtcggac
     5521 acgtcttgtg g
//
LOCUS       DASWAV010000005         5523 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U2665
            k141_680841, whole genome shotgun sequence.
ACCESSION   DASWAV010000005 DASWAV010000000
VERSION     DASWAV010000005.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960316
            Sequence Read Archive: SRR10260007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 5523)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5523)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:47:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,929
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5523
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_680841"
                     /isolate="SMAG_U2665"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.36444 N 132.31889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..957
                     /locus_tag="VF958_00075"
     CDS             <1..957
                     /locus_tag="VF958_00075"
                     /inference="COORDINATES: protein motif:HMM:NF012933.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9147529.1"
                     /translation="KIGQLAALAADRGLAVKRFSVVPPTPYSLALTAQRYTRFADPVD
                     HFDGTTYGRLLSVGRETLLVAVEQSGGATNPKLRVTLEGPGEDSPRARVAADRLVKGA
                     LGAGTDVRLFSRAARLDPLLAPFVRRFRGLRIAGYPSLWEALVTAVLSQQVNLSLAFG
                     IRAQLATEFGRRRKVRGETYFAFPLPEAIAEAGERGLGGFRMSSSKIGTVARLARAFA
                     SGELSEEVIAALPEEAAVERLTEMKGIGRWTAETALLRGLAREDAFPAGDLGIMKYLA
                     EGLFEHGRRASESEMRLYAERWRPWRGLALVYCWAELARRKT"
     gene            980..1150
                     /locus_tag="VF958_00080"
     CDS             980..1150
                     /locus_tag="VF958_00080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9147530.1"
                     /translation="MTRTIQLADEEAEILKSVLEEYISDLRMEVSNTDSMDLREQLKR
                     KEEVLKRLAGLL"
     gene            1160..2167
                     /locus_tag="VF958_00085"
     CDS             1160..2167
                     /locus_tag="VF958_00085"
                     /inference="COORDINATES: protein motif:HMM:NF024989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HEX9147531.1"
                     /translation="MSRAWSVERFGGPEQLRLAEREDLSPGVGEVAIRTAAIGINFAD
                     LFARAGVYPNTPRPPFVAGMELSGIVESVGPGVSDLAPGLRVVAVPIFGGYAERVVCR
                     ASQVFALPDGSDLVEAAAMPVAFLTARYAIDEARVRPGERVVVTAAAGGVGTALLQLL
                     ALRGAETMALVGSEKKFAICRELGAAEVALYGAAGRIPAVAGSRIDVVIDAIGGEAFR
                     ALWRRLAPGGRYVLYGFAAAAGKKGVARLRAAREFIAMGFLAPYFFVQSCRTLIGFNL
                     SLLPNRLAELREGADEIFREWQAGRLRPIIGPRFDFERLPDAHRALASRKTQGKVVVT
                     V"
     gene            complement(2140..3360)
                     /locus_tag="VF958_00090"
     CDS             complement(2140..3360)
                     /locus_tag="VF958_00090"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021134.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 87 protein"
                     /protein_id="HEX9147532.1"
                     /translation="MSRVLLALAGTTLVVLGIRTVLVGAVDSRLRSDFPVYWLAGRAV
                     WEGKNIYSVAGPHGWEYIYPPPFAIAMVPFGWMPLFWAVIVWFLVSALLTAWAVQMCV
                     TMTKERLPPGTDVLALYVLPGLLLLGWYASAIVRGQASVPLLWLIVAAIFWQSRGRQI
                     AGASCLGGAILMKVFPALLLLYFMWRRRWRFVIATLALLLVGGLALPAVVIGTRRNLE
                     YLRTWGTIVALPSLRTHHAESESALDSQLLDFRKRRNESLYAVLRRLTHTRRAREWAA
                     GIALAMGGVMWMAGRRAPPEAELPLLSAAVAWTVLAIPVSWSHYFILLLLPLTVLVAA
                     AAQKDDRAMRPVALAALILFGVGSLVAIVFKGKLETYGTLCWATLGVWGALVLAATRR
                     PRLPTDHTVTTTFP"
     gene            complement(3493..3708)
                     /locus_tag="VF958_00095"
     CDS             complement(3493..3708)
                     /locus_tag="VF958_00095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9147533.1"
                     /translation="MITVEDFTALSAGSDVPCEGHVGECPKCGRNGVRQSEPERTFYV
                     HVQDSEMLSDGMRTEPRDWCRVEKLAV"
     gene            3758..4396
                     /locus_tag="VF958_00100"
     CDS             3758..4396
                     /locus_tag="VF958_00100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013032743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta family hydrolase"
                     /protein_id="HEX9147534.1"
                     /translation="MRSDSDLLFDGSDDALTTVVLAHGAGAPMNTPFMNSVARGLAAG
                     RGVRVVRFEFPYMRARRETKRRTGAPDREPVLRNSWKEAVEKLGGARKLVIGGKSMGG
                     RIASLVADEVGARGLVCLGYPFHPPGRPEKLRTKHLETLRTPALVVQGTRDPFGTPED
                     VAGYALSPAIRVVWLEDGDHSWKPRASSGRTEAQNTADGIAAILDFLGALNS"
     gene            4482..4889
                     /gene="msrB"
                     /locus_tag="VF958_00105"
     CDS             4482..4889
                     /gene="msrB"
                     /locus_tag="VF958_00105"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011020500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033743 - peptide-methionine (R)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HEX9147535.1"
                     /translation="MDKVEKSDEAWRRALTPEQYHVMRQKGTERPFTGEYEKAKEKGT
                     YVCAACGNPLFSSETKYESGSGWPSFYAPLSNESVATAADNAHLMERTEVLCSRCDSH
                     LGHVFDDGPPPTGQRYCMNSIALKLEKEGEEKK"
     gene            4886..5365
                     /locus_tag="VF958_00110"
     CDS             4886..5365
                     /locus_tag="VF958_00110"
                     /inference="COORDINATES: protein motif:HMM:NF025887.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4440 domain-containing protein"
                     /protein_id="HEX9147536.1"
                     /translation="MRRTLGTAAAFVLASAALLAHDAAADVEAAKRAIKLADLELAKA
                     VADRSLQSFVSMVDDDAVFFGKDVSRGKDAVSKAWLPMFTDRNLFLKWHPNEVYVSSS
                     GDLGYSIGDYERIGKDTSGNLEIASGSYVSIWRRQPDRKWKIVLDIGSPATPTPQKP"
BASE COUNT          956 a         1850 c         1866 g          851 t
ORIGIN      
        1 aaaatcgggc agctcgccgc tctcgcggcg gatcgcggac tcgcggtgaa gcgcttctcc
       61 gtcgtgccgc ccaccccgta ctcgctcgcg ttgacggcgc agcggtacac gagatttgcc
      121 gaccccgtcg accacttcga cgggacaacc tacggccgac tcctctcggt cgggcgcgag
      181 actctgctcg tcgcggtcga gcagagcgga ggcgcgacga acccgaagct tcgcgtcacg
      241 ttggaaggtc cgggagagga ctccccccgc gcgcgcgtcg cggcagaccg actggtgaag
      301 ggcgcgctcg gtgccgggac cgacgtgcga ctcttctcgc gggcggcccg gctcgatccg
      361 ctgctcgcgc cgttcgtgcg ccggtttcgc gggctgcgga tcgccggata tccgtcgctg
      421 tgggaggcgc tcgtgaccgc cgttctctcg cagcaggtca acctgtccct cgcgttcggg
      481 atccgcgcgc aactcgcgac cgagttcggc cggaggcgga aggtgcgcgg cgagacgtac
      541 ttcgcgtttc cgcttcccga ggcgatcgcg gaggccgggg agcgggggct cggaggcttc
      601 cgcatgtcgt cgagcaagat cggaactgtt gcacgtctcg cgagggcgtt cgcgagcggc
      661 gagctctccg aggaagtgat tgccgccctc cccgaggaag cggcggtcga gcgattgacc
      721 gagatgaaag ggatcgggcg ctggaccgcc gagactgcgc tgctgcgcgg ccttgcgcgg
      781 gaggacgcgt tccccgcggg cgacctcggg atcatgaagt acctcgcgga gggtctgttc
      841 gagcacggcc ggcgagcaag cgaatccgaa atgcggctct acgcggagcg gtggcggccg
      901 tggcgcggcc tggcgctcgt ctattgctgg gccgaactcg cgcgacgaaa gacataggat
      961 cgaaaccggg aggagaagaa tgacccgaac gattcagctc gcggacgagg aggcggagat
     1021 cctgaaatcc gttctcgagg agtacatctc cgacctccga atggaggtct cgaacaccga
     1081 cagcatggac ttgcgcgaac aactcaagcg caaggaggaa gtcctgaagc gcctcgcggg
     1141 tctgctgtag aggttcttga tgtcgcgtgc ctggtccgtc gagcggttcg gcgggccgga
     1201 gcaactgcgg ctcgcggagc gcgaggacct ctcgccgggc gtcggagagg tcgcgatccg
     1261 aacggccgcg atcgggatca acttcgccga cctcttcgcg cgcgccggcg tctacccgaa
     1321 cacgccgcgc cctccattcg tcgcgggcat ggagctctcg ggtatcgtcg agtccgtcgg
     1381 gccgggggtc tccgacctcg cgcccgggct ccgcgtcgtc gcggtcccca tcttcggcgg
     1441 ttacgccgag cgcgtcgtct gccgtgcatc gcaggtcttt gcgctccccg acggctccga
     1501 cctcgtcgag gctgcggcga tgccggttgc gttcctcacc gcgcgctacg cgatcgatga
     1561 agcgcgggtg cgcccggggg aaagagtcgt cgtcacggcc gcggccggag gcgtcgggac
     1621 ggctctcctg cagctcctcg cgctgcgtgg agcagagacg atggccctcg tcgggtcgga
     1681 aaagaagttc gcgatctgtc gcgaactcgg agccgccgag gtcgcgctgt acggcgcggc
     1741 gggtcggatc ccggcagtcg cgggctcacg gatcgacgtc gtgatcgacg cgattggcgg
     1801 ggaggctttc cgcgcgctgt ggcggcggct ggcgcccggc gggcgctacg tcctctacgg
     1861 gttcgcggcg gccgccggaa agaagggcgt cgcccggctg cgcgccgcgc gagagttcat
     1921 cgcgatggga ttccttgcgc cgtacttctt cgtgcagtcc tgccgcactc tgatcggctt
     1981 caacctgtct ctgctcccga atcgcctcgc ggagctgcgg gagggagccg acgagatttt
     2041 tcgcgagtgg caggccgggc gcctgcgtcc gatcatcggc ccacggttcg atttcgagcg
     2101 gctgccggac gcgcatcgcg cgctcgcgag ccgcaagact cagggaaagg tggtcgtgac
     2161 ggtgtgatct gtggggaggc gcggccgtcg ggtcgcggcc agcaccagag cgccccagac
     2221 gccgagcgtt gcccagcaga gagtcccgta agtctccagc ttgcccttga aaacgatcgc
     2281 cacgagactc cccaccccga aaaggatcag tgccgccagc gcaacgggcc gcatcgctcg
     2341 gtcatccttc tgagccgcgg cggcgaccag gaccgtcagc ggaaggagca gcaggatgaa
     2401 atagtgggac caggagaccg gaatcgcgag caccgtccac gccaccgctg ccgacaacag
     2461 ggggagttcg gcctccggag gtgcccgacg ccccgccatc cacattaccc cgcccatcgc
     2521 cagcgctatc cccgctgccc actcgcgggc tcgccgtgtg tgcgtcagcc ggcgcagaac
     2581 ggcatagagc gactcattcc gccttttcct gaagtcgagc agctgactgt ccagagcgct
     2641 ctcggattcc gcgtgatgcg tgcgcaagct cggaagagcg acgatcgtcc cccacgtgcg
     2701 caaatactcc aggttcctcc gagtgccgat gacgacggcc ggcagcgcca gtccgcccac
     2761 cagcaggaga gccagcgttg cgatcacgaa gcgccatcgc ctgcgccaca tgaagtacag
     2821 aaggagcagc gccggaaaca ccttcatgag gatcgctccc cccaggcagg acgcgcctgc
     2881 gatctgtcga cctcgcgatt gccagaagat cgccgctacg atcagccaga gcagcgggac
     2941 cgacgcctgc ccgcgcacaa tcgccgacgc ataccatccg agcaggagca ggcccggcag
     3001 cacgtagagc gcaaggacgt ccgttcccgg cggaagccgc tccttcgtca tcgtgacgca
     3061 catctgaacg gcccacgcag tcaggagagc cgagacaagg aaccagacga tgactgccca
     3121 gaagagtggc atccatccga acggaaccat cgcgatcgcg aacggcggtg ggtagatgta
     3181 ctcccaacca tggggtccgg cgaccgaata aatgttcttt ccttcccaga ccgcccggcc
     3241 cgcgagccag tacaccggaa aatccgaccg taacctcgaa tccaccgctc cgacgaggac
     3301 cgttcggatg cccaggacga ctaacgtcgt gccggccagc gccagcaaga cccgactcaa
     3361 ccgtcgccgc tcgagcattc gactcgactg cctcgtcgag tggaagtctg tcacgcgatc
     3421 ggcgcacgcg cggggacgcc cgagtctcta cttcgagcag gggagacgga ttccgggggc
     3481 ggaggttacg agctagacgg cgagtttttc gacgcggcac cagtcacgcg gctcggtcct
     3541 catgccgtcc gagagcatct ccgaatcctg gacgtggacg tagaaggtcc tctccggctc
     3601 gctctgccgg actccattgc ggccgcattt cgggcactcc ccgacgtgac cttcacaggg
     3661 cacgtcgctt ccggcggaaa gggcggtgaa gtcttcgacc gtgatcatgc aagcactctg
     3721 tctgcaagag tcgttccgtc gctaacatcc tcagacgatg agatccgact cggatttgct
     3781 cttcgacggg tccgatgacg cgctgacaac cgtcgtcctc gcccacggcg cgggcgcgcc
     3841 gatgaacacg cccttcatga actccgtcgc gcgcggcctg gcggccggca gaggcgttcg
     3901 cgtcgtccgc ttcgagttcc cgtacatgcg cgcgcggcgc gagacgaagc ggcggaccgg
     3961 tgctccggat cgggagcccg ttctccgaaa ctcgtggaaa gaggccgtcg aaaagctcgg
     4021 cggcgcccgg aagctcgtca tcggcggcaa gtccatgggg ggccgcatcg cgagcctggt
     4081 cgcggacgaa gtcggtgccc gcgggctcgt ttgcctcggg taccccttcc acccgccggg
     4141 acgtcccgag aagctgcgca ccaagcatct cgagacgctc cgaacgcccg cgctcgtcgt
     4201 ccagggcacg cgcgacccgt tcggcacccc cgaagacgtc gccgggtacg cgctctcgcc
     4261 tgcgatccgc gtcgtctggc tcgaagacgg cgaccactcc tggaagccgc gggcgtcgtc
     4321 gggccggacc gaggcgcaaa acacggcgga cgggatcgcg gccatcctcg atttcctggg
     4381 ggcgctgaat tcctgacggc aaggcttctt gacccatgtg gcaagtggag acctgacccc
     4441 caatgtggca agtggagacc tgacccccat agacttactc catggacaag gtcgagaaat
     4501 ccgacgaagc gtggcgccgc gccctcactc ccgagcagta ccacgtgatg cggcagaagg
     4561 ggacggagcg cccgttcacc ggcgagtacg aaaaagcgaa ggagaagggg acgtacgtgt
     4621 gcgccgcctg cggtaacccc ctcttcagct ccgagacgaa gtacgagtcc ggcagcggct
     4681 ggccgagctt ctacgcgccg ctgtcgaacg agagcgtcgc gaccgcggcc gacaacgctc
     4741 atcttatgga acgcaccgag gtcctctgca gccgctgcga ctcgcacctc gggcacgtgt
     4801 tcgacgacgg gcctccgccg acgggacagc gctactgcat gaactcgatc gcgctgaagc
     4861 tcgagaaaga aggggaggaa aagaagtgag aaggacactc gggaccgcag ccgcgttcgt
     4921 cctggcgagc gccgcactcc tggcgcacga tgccgcggcg gacgtcgagg ccgcgaagcg
     4981 agcgatcaag ctcgccgacc tcgagctcgc gaaggccgtg gcggaccgca gccttcagag
     5041 tttcgtctcg atggtggacg acgacgccgt cttcttcggc aaggacgtct cgcgcggcaa
     5101 ggatgccgtg tcgaaggcct ggctgccgat gttcaccgat cggaacctgt tcctgaagtg
     5161 gcacccgaac gaggtctacg tatcgtcctc cggcgacctc ggttactcga tcggcgatta
     5221 cgagcggatc ggaaaggaca cgtccggtaa tctcgaaatc gcgagcggca gttacgtctc
     5281 gatctggcgc aggcagccgg accgcaagtg gaagatcgtt ctcgacatcg gctctcccgc
     5341 gacgccgacg cctcagaagc cgtagcgctc cgctggctcc tggcgggcga tcccgccgtc
     5401 cggtggcagg cactgcgaga tctcgtcggc gccgacagca gggaagtctc gcgcgagcgg
     5461 cgccggacga cgcgcgaggg ctgggtcgcg cgcctcctcg cgaaacagga cgcggacggc
     5521 cgg
//
LOCUS       DASWAV010000006         1745 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U2665
            k141_456, whole genome shotgun sequence.
ACCESSION   DASWAV010000006 DASWAV010000000
VERSION     DASWAV010000006.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960316
            Sequence Read Archive: SRR10260007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 1745)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1745)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:47:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,929
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1745
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_456"
                     /isolate="SMAG_U2665"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.36444 N 132.31889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            50..484
                     /locus_tag="VF958_00115"
     CDS             50..484
                     /locus_tag="VF958_00115"
                     /inference="COORDINATES: protein motif:HMM:NF025852.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4437 domain-containing protein"
                     /protein_id="HEX9147537.1"
                     /translation="MKAFTRVTPLVLIGLIVALTLSAAGGGKGVAMPNGEMKWVENPA
                     MKGLSVATLWGDPSKGAYGALKKVAGGTDLGFHSHSFDQKVIAVSGTFDFQFKGEGVK
                     ELGPGSYVSTPAHVVHSAKCRAGADCVYFEESAGKSDYIPAK"
     gene            complement(510..>1745)
                     /locus_tag="VF958_00120"
     CDS             complement(510..>1745)
                     /locus_tag="VF958_00120"
                     /inference="COORDINATES: protein motif:HMM:NF013188.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-binding protein"
                     /protein_id="HEX9147538.1"
                     /translation="GAFAAGVVVGDTEFIHEISGILRPFRDFLSVLFFASIGMLLDPA
                     FVVSALPLVLATVGAVVFIKVVAAYPAFRASGALGRTSLRAAFAIAPIGEFSFLLAQE
                     GKRFGVLQPEDEQLFVATAVITLGATPLLVAVGRVLAERMRGHPEPVEEVTGRELSGH
                     IVIIGYGLNGLNVAHVLTETKIPHVVIEEDPHRAQIARANGSRVVVADAADPSGLEAA
                     GIDRALAVVVAISDPDGTRSIVRACRQANPNVHIIVRTRYVAQVEPLRELGANEVIPE
                     EFETSVEIVSRLMRLLAVPGNVVAAQIRVLRDEAYRLLRDPAMRAAEGRRLSAALEAG
                     TSQTFFVLPDTPALGKTLSEMGVADSRVAVPAMIRRGAPYSPAPTDEPLQAGDTLFLV
                     GAHEDLMKVMARLEGTRAS"
BASE COUNT          304 a          584 c          599 g          258 t
ORIGIN      
        1 cgtgtaggat gcgcgcaaaa ccgtccgata acgaaaagga gaaagcttca tgaaggcatt
       61 cactcgggtc acacctctgg ttctcatcgg gttgatcgtc gcgctcactc tctccgcggc
      121 cggcggaggg aagggagtgg caatgccgaa cggcgaaatg aaatgggtgg agaatcccgc
      181 catgaaaggg ctttcggtcg ccacactctg gggtgatccg tccaagggag cgtacggcgc
      241 gctcaagaag gtcgccgggg gaaccgatct cggcttccac agtcactcct tcgaccagaa
      301 ggtgatcgcc gtttcgggca cgttcgactt tcagttcaaa ggcgagggcg tgaaggagct
      361 cggcccgggc tcctatgtat ccacgcccgc gcacgtcgtg cactcggcga agtgtcgcgc
      421 cggagccgac tgcgtgtact tcgaagagtc ggcgggaaag agcgactaca tccccgccaa
      481 gtagagttcg ttcgaaggcg ggcgtccgat caggacgccc gcgttccttc cagccgcgcc
      541 atcaccttca tcaggtcttc gtgcgcgccg accaagaaca gcgtgtcgcc cgcctggagg
      601 ggctcgtcgg tcggggcggg agaatacggc gcgccgcgtc ggatcatcgc cggcaccgca
      661 acgcgcgagt cggcaacgcc catctccgag agcgtcttcc cgagcgccgg cgtgtccggc
      721 agcacgaaga acgtctggga cgtcccggct tcgagcgccg cggagaggcg tcggccctcg
      781 gccgcgcgca tggcggggtc gcgcagcaac cggtaggcct cgtcgcgcag cacgcggatc
      841 tgcgcggcga cgacgttgcc gggcaccgcg aggagccgca tgaggcgcga gacgatctcg
      901 acggacgtct cgaactcctc cgggatgacc tcgttcgcgc cgagttcgcg cagcggctcc
      961 acctgcgcca cgtagcgtgt cctcacgatg atgtggacgt tcgggttcgc ctggcggcag
     1021 gcgcgcacaa tgctccgcgt tccgtccggg tcggagatcg cgaccacgac ggcaagcgcc
     1081 cggtcgatgc cggcggcctc gaggccgctc gggtcggcgg cgtccgccac cacgacgcgg
     1141 ctcccatttg cccgcgcgat ctgcgcgcgg tgcgggtcct cctcgatgac gacgtgcggg
     1201 atcttcgtct ccgtcagcac gtgggcgaca ttcagtccgt tcaggccgta gccgatgatg
     1261 acgatgtggc cggagagctc gcgccccgtc acttcttcca cgggctccgg gtggccgcgc
     1321 atccgctccg ccagaacacg cccgacggcg accaggagcg gcgtggcgcc gagcgtgatg
     1381 accgccgtcg ccacgaacag ctgctcgtcc tccggctgga gcaccccgaa gcgcttcccc
     1441 tcctgcgcga gcaggaacga gaactctccg atcggcgcga tcgcgaatgc cgcccgcagc
     1501 gacgttcgac ccaacgctcc ggacgcgcgg aacgcgggat acgccgccac caccttgatg
     1561 aagacgaccg cgccgacggt ggcgaggacg agcggcaggg ccgagacgac gaacgccggg
     1621 tcgagcagca tcccgatcga cgcgaagaag agaaccgaga ggaagtcgcg gaacgggcgg
     1681 agtatgccgc tgatctcgtg gatgaattcc gtgtcgccga cgacgacgcc ggcggcgaag
     1741 gcgcc
//
LOCUS       DASWAV010000007        10243 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U2665
            k141_480546, whole genome shotgun sequence.
ACCESSION   DASWAV010000007 DASWAV010000000
VERSION     DASWAV010000007.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960316
            Sequence Read Archive: SRR10260007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 10243)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10243)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:47:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,929
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10243
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_480546"
                     /isolate="SMAG_U2665"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.36444 N 132.31889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..215)
                     /locus_tag="VF958_00125"
     CDS             complement(<1..215)
                     /locus_tag="VF958_00125"
                     /inference="COORDINATES: protein motif:HMM:NF023663.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9147539.1"
                     /translation="MSLQRTSGWRQRRAGTSARANIRAVLAGDTQTRWALVLGASSGF
                     GEACSIALAKAGWNIFGVHLDRKSTQP"
     gene            234..1601
                     /locus_tag="VF958_00130"
     CDS             234..1601
                     /locus_tag="VF958_00130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9147540.1"
                     /translation="MNFRRFAAVSLVSLASASAQGQGAARQEVVVTNQNLALVTESRA
                     IPLPAGRTEVAWDGAPASARTETWSVTNADEAGVKWLGLTSPLPGQTATGTEWLAGLV
                     GKRVRIERPGGATVEGDVLAVHGPTPAQVLFREGGEYVYGEPDARISLTVDANQTVRP
                     TGVVLKLDSARAGTRALTSRYLVGDVTWEASYALSLAPDEKRGRLEGFFVVDNRSGAD
                     FTPSRLRLLAGTLRVASGPSSPVPMVMRAQATVAEDSMAKGAELSESRIYDVESPPVL
                     AMGRTTFPLASGADVAVEKRYLARTTYWFGAMEESQRVPVGVQYRVETKPLAHALPAG
                     VVRVYAQGVFTGEDRIGHTPERTDIEIESSEAFDLSARRRQVSFQQPGPRETDSAYEI
                     VITSRKKDAVTVLVREQFPGDWTIVESSVPSKKISAYTAEFAVPVPAGGEAKLTYRVK
                     VRMPG"
     gene            complement(1602..3485)
                     /locus_tag="VF958_00135"
     CDS             complement(1602..3485)
                     /locus_tag="VF958_00135"
                     /inference="COORDINATES: protein motif:HMM:TIGR03436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HEX9147541.1"
                     /translation="MLPKIRFMATFLALAALSLPAQTRKTELKTPAGRFPLEVTAEYL
                     GMAAGKTVVRIRLASAELSKAAAAKGIRAFSGELRGTFSRGDEMVQAFRYPVSGDVMD
                     GKPFTYSFLRPIAPGNYRLKLVVAEPGGREIGEGAVDLMVPEVGLAFRPEMAPAEAET
                     LPEAEAIVIADEAAPRETGKTDSDTPKLKLLPPPREAPVGLLRLDAQIEPPITKVAFY
                     LGEKLLLARTRPPYSVEIDLGNLPLRQTIRAVGYDDTGRVIDEDAWAINEGAARVAVR
                     VLPQPDPSAGKVRVKVAVQSIAGGIAKLVELFLDERKIGSWTHGPYVATIPYDQYARG
                     NFLRATAVSEDGREANDIRMLKGPSTTVESVRVDVVQLHVSALDKAGHFVKGLAREDF
                     SIQEDGRSQTISGFEVADKLPLTVGLVVDGSGSMEKGLPFVHDASAELFKNLIREKDR
                     GFVIEFRERPRFLQEITGNSGDLQRAARETRAEGATALYDSIILGLYQFRALEGRKAL
                     IVVTDGADNRSHVDFATLLRYARSAGAPIYFIAVNIPLTDFKSKKVIGEIATESGGQV
                     FSIGSAAKIGEVTKQIEEELRSQYILAFRTDSQKPQGEYRAVNVAVAKPGISIRTIRG
                     YIP"
     gene            complement(3551..4537)
                     /gene="dusB"
                     /locus_tag="VF958_00140"
     CDS             complement(3551..4537)
                     /gene="dusB"
                     /locus_tag="VF958_00140"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HEX9147542.1"
                     /translation="MIRLGSVTVDPPLVLAPMAGITDHLYRLMLRRIGGVGLVTMEFI
                     SSEAITRGNARHRAKMFFSDEERPLSIQIYGSNADRMAAAAEIVEELAPDVCDINMGC
                     PANKVLKGCAGAALMGDLPLARKIVREVKSRLSIPLTVKFRLGLDDPRKNFLDLGRIC
                     EDEGAAAVAMHARTARQMYAGNADRGEIAQLKRALSIPVIGNGDVETADDVLAMLRDT
                     GCDGVMIGRATMKNPWIFRQAADRLAGGTPREATAAERRDLMLWHFGQIEATARDARE
                     ALHKLKTMTGWYTHGLPNGRALRVRISELETPADFREAVVRFFEGSPLAATA"
     gene            4606..5679
                     /locus_tag="VF958_00145"
     CDS             4606..5679
                     /locus_tag="VF958_00145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9147543.1"
                     /translation="MAVSGATISGGARESLWIRSRRFDLLFLTLSGVLVFLPYLSYGL
                     LQRLGASPGTASLIVGLTVTLLVGGPHMYSTYLRTALEPRFRARYGFLAYLPLLLIPV
                     LVILGALHAFLLLITAFFFWASLHVTQQAQWIAETYRRKATGRVSMVDRLLDGAVILG
                     ALYTMAMYKFVEGRFALGDSTLLFPEFLKRREIAIAFTVGFAALFLFYVFRTAAEIRR
                     GEASVPRLLFMAFTVGLAFVVPIFDNLDVAFQGFNTWHSLQYLALTWFILSRNAERGA
                     IGSSLAQKLAGTEKTGRFYGAMILATVMAGGIYLVLWKGLGFPQDKSYYVVVLSFLLI
                     HYFYDHFLFRDFAPLDAREGARA"
     gene            complement(5676..6314)
                     /locus_tag="VF958_00150"
     CDS             complement(5676..6314)
                     /locus_tag="VF958_00150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9147544.1"
                     /translation="MPMSPRLFLHSAAAAAILLAIACGGPKTVSRDGYRAVLVYSSED
                     RFRIAVRGESVWISGNVDGSEIVKIVRPDLHKVWQLRPSTKKIYETRWEPTDEAVPGY
                     PLQSKFDVAAYADRFGGKAERQDDATHGLHPCERWRLALPSGDVATIWVARDLDRLVV
                     RIEHAKKDPGDEYQPFTDTQLLDLRVGASAKLFEPPKGYTKVSSYEELTKKT"
     gene            6420..8003
                     /gene="bshC"
                     /locus_tag="VF958_00155"
     CDS             6420..8003
                     /gene="bshC"
                     /locus_tag="VF958_00155"
                     /inference="COORDINATES: protein motif:HMM:NF021586.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacillithiol biosynthesis BshC"
                     /protein_id="HEX9147545.1"
                     /translation="MSTIPLEKYPGLPALFVDFARGRSGLHPDPPTLDVAVARGRELL
                     SRRDAPRLPVSAFRHRSPAGPAAAESLASGRAVAVLAGHQIGLFTGPLFTIVKALDAV
                     AFARELSRRGVPAVAVFWALTDDHDLEEIARTARPGPDGPDKFLLEGADRSNRRPVGA
                     LRIPEKIAGVVEAFRADARGPEALEILDGFARRYAAGRTYAEAFVDALFDLTGDDPVL
                     VVDPLGPEVAEAARHFFLAAAERRDEVARILAGGAHRVAEAGREPPVPYRADLFPFFI
                     VEEGSRRRVRAGEIDEAAKKVERGEARVSADVLTRPVLKSLVLPAAVSVLGPSEIAYH
                     AQSLPLFPLFGANPPVLMPRSLLVLRGPAERRAQQALGVTDEDLLTPGAARASAGPVP
                     QAEQVAEIARRLDAELASVGSEVGGIDPTLTGALETARRKAAYQLEQLAERVRKAAER
                     KDEVTLQRRRRLETMVLPGGDAAERVYPPLVFLLAWGRTLLDTLRGAAGRGAADAAIV
                     DVETAAGAGAEMTRTTHAG"
     gene            7993..8715
                     /gene="bshB1"
                     /locus_tag="VF958_00160"
     CDS             7993..8715
                     /gene="bshB1"
                     /locus_tag="VF958_00160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020595618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019213 - deacetylase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bacillithiol biosynthesis deacetylase BshB1"
                     /protein_id="HEX9147546.1"
                     /translation="MPVDVLAIAAHPDDVEMTCGGTLLRLKERGKKFGIVDLTAGEMG
                     TRGTSETRRLEAERAAEILGAEFRETLDFGDGGLVTSREHELVLMDVIRREKPRLILT
                     SYPDDRHPDHSRAGRFVTDAAFYAGLRKVETNHPAHRPQHTIYFPTAVMHEPDFVVDI
                     TAVMETRRAAILAFASQFHREGSTEPETMLSRKMFLEALDARARHVGFLGGVEFGEGF
                     LSKRPPRIDDLIGAFEGLEPGF"
     gene            8735..9859
                     /gene="bshA"
                     /locus_tag="VF958_00165"
     CDS             8735..9859
                     /gene="bshA"
                     /locus_tag="VF958_00165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020709804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0071793 - bacillithiol biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-alpha-D-glucosaminyl L-malate synthase
                     BshA"
                     /protein_id="HEX9147547.1"
                     /translation="MKIGITCYPTYGGSGVVATELGLALADRGDEIHFISYDLPSRLA
                     LPRPRVYFHEVVAPTYPLFVSPPYTLALATKMAEVAEHVRLDLLHVHYALPHAISAIL
                     AREMSDGNGVRLKVVTTLHGTDITIVGQDRSYLPITRWGIEQSDAVTAVSRYLTDMTL
                     RELGVRRTVETIPNFVDPNRYRPDGASAYARTLVRDDEVGVIHVSNFRPVKRIPDVLA
                     VFDRIRREVPARLLLIGDGPERSLAERIARQRGFEDRATFLGNVAAIETIFPVAKVML
                     LPSDAESFGLAALEAMACGVPVVGTAAGGLPEVVADGACGYLRPVGDVEGMAEAALTL
                     LRDPEKWHRFSSEARRRAVEEFPTDEIVSRYRKLYEATLG"
     gene            complement(9838..>10243)
                     /locus_tag="VF958_00170"
     CDS             complement(9838..>10243)
                     /locus_tag="VF958_00170"
                     /inference="COORDINATES: protein motif:HMM:NF013155.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9147548.1"
                     /translation="LRPDRVDHGIAAANDAHLLARFAEESTPLWVSPTSNVVTGAVQD
                     LGDHPLPRLLDAGVRVAIGADDPLLFGTTTAQEHALVRERFGFGDRTMRLLAENSWRA
                     SFCPPADRERALVELGTRRPRREGPPQPSVAS"
BASE COUNT         1537 a         3553 c         3469 g         1684 t
ORIGIN      
        1 ttcggttgcg tggatttgcg gtcgaggtgg acgccgaaga tgttccagcc cgccttcgcg
       61 agcgcgatcg agcaggcctc gccgaagccg gacgaggcac cgagaacgag ggcccaccgc
      121 gtctgcgtat cgccggccaa aacggcgcgg atgttagcac gcgcagatgt gccggcacgc
      181 ctctgccgcc accccgatgt gcgctgtaaa ctcatttctt ggagggtctt aggatgaact
      241 tccgccgctt cgccgccgtc tctctcgtgt cccttgcgag cgcgtccgcg cagggtcagg
      301 gggcggctcg ccaggaggtc gtcgtcacga accagaacct cgcgctcgtg accgagagcc
      361 gcgcgatccc gctccccgcc ggacggaccg aagttgcctg ggacggagcg ccggcatcgg
      421 cccgcaccga aacctggagc gtgacgaacg cggacgaggc gggggtgaag tggctcgggc
      481 tgacgtcacc gctgccgggc cagacggcca caggtaccga gtggctggcg gggctcgtcg
      541 gaaagcgcgt ccgaatcgag cgtcccggcg gagcgacggt cgagggggac gttctcgctg
      601 tgcacgggcc gaccccggct caggtcctct ttcgtgaggg gggcgagtac gtctacggag
      661 agccggacgc gcggatctcg ctcacagtgg acgccaacca aacggtgcgg ccgaccggcg
      721 tggtactgaa gctcgacagc gcccgcgcgg gcacgcgggc gctcacgtct cggtatctcg
      781 tcggcgacgt gacgtgggag gcgagctacg cgctttcgct cgcgcccgac gagaagcgcg
      841 ggcggctcga gggtttcttc gtcgtcgaca accggagcgg cgcggacttc acgccctcac
      901 ggctgcgact gctcgcgggg acgctccgcg tggcgagcgg gccgtcgtcc ccggtgccga
      961 tggtgatgcg cgcacaggca accgtcgcgg aagactcgat ggcgaagggc gcggagctct
     1021 ccgagtcccg catctacgac gtcgagtcgc cccccgtgct cgcgatgggc cgcacgacgt
     1081 ttccgctcgc gtcgggcgcc gacgtcgcgg tcgagaagcg ttacctcgcc cgcacgacgt
     1141 actggttcgg cgcgatggaa gaatcccagc gggtcccggt gggcgtccag taccgcgtcg
     1201 agacgaagcc gctcgcgcac gctctgcccg ccggcgtcgt gcgcgtgtac gcccagggcg
     1261 tcttcaccgg cgaggaccgg atcggccaca ctcccgagcg gacggacatc gagatcgaga
     1321 gctccgaggc gttcgatctc tccgcgcggc ggcgccaggt ctccttccag cagccgggcc
     1381 cgcgggaaac cgactccgcc tacgagatcg tgatcacgag ccgcaagaag gacgcagtca
     1441 ccgttctcgt ccgcgagcag ttcccgggcg actggacgat cgtcgagtcg tcggttccgt
     1501 cgaagaagat ctcggcgtac acggcggagt tcgcggtgcc cgtgcccgcg ggcggcgagg
     1561 cgaagctgac ctatcgggtc aaggtgcgga tgccgggata gctaaggaat gtagccgcgg
     1621 atcgtccgga tcgagatccc cggcttcgcc accgcgacgt tcacggctcg atactccccc
     1681 tgcggcttct gggagtcggt gcggaacgcg aggatgtact gcgaccgcaa ctcctcctcg
     1741 atctgcttcg tcacctctcc gatcttcgcg gcgctcccga tcgagaagac ctgcccgccc
     1801 gattccgtcg cgatctcgcc gatcaccttc ttcgacttga agtcggtcag cgggatgttc
     1861 acggcgatga agtagatggg cgctcccgcc gagcgcgcgt accggaggag cgtcgcgaag
     1921 tccacgtgcg accggttgtc ggcgccgtcg gtgacgacga tgagcgcctt gcgcccttcg
     1981 agcgcgcgga actggtagag gccgaggatg atcgagtcgt agagcgccgt cgcgccctcg
     2041 gcgcgggtct cgcgcgccgc ccgttggagg tcgccggagt tccccgtgat ctcctgcagg
     2101 aaacgcggcc gctcccggaa ctcgatgacg aagccacgat ccttctcccg gatcaggttc
     2161 ttgaacagct ccgccgacgc gtcgtgcacg aacggcaaac ccttctccat cgaaccggag
     2221 ccgtccacga cgaggccgac ggtcaacggg agcttgtcgg cgacctcgaa gccggagatc
     2281 gtctggctgc ggccgtcctc ctgaatcgag aagtcctcgc gcgcgagtcc cttcacgaag
     2341 tgacccgcct tgtcgagcgc ggacacgtgg agctgaacga cgtcgacccg cacgctctcg
     2401 acagtcgtgg acggaccctt cagcatccgg atgtcgttcg cttccctgcc gtcctcggag
     2461 accgcggtcg cacggaggaa gttcccgcgg gcgtactggt cgtacggaat ggtcgcgacg
     2521 tacggaccgt gggtccagga gccgatcttc ctctcgtcga ggaagagctc gaccaacttc
     2581 gcgataccac ccgcgatcga ctgcacggcg accttgacgc gcactttccc ggcggacggg
     2641 tcgggctgcg gcagaacccg cacggcgacg cgcgccgccc cttcgttgat cgcccacgcg
     2701 tcctcgtcga tgacccgccc ggtgtcgtcg tagccgaccg cgcggatcgt ctgacgcaga
     2761 ggcaggtttc cgagatcgat ctcaacggag tacggcgggc gcgtccgcgc gagaaggagc
     2821 ttctctccga gatagaaggc gactttcgtg atcggcggct cgatctgcgc atcgagccgc
     2881 agcaggccca ccggagcctc gcgcggaggg ggcagaagct tcaacttcgg cgtatccgag
     2941 tcggtctttc ccgtttcccg cggcgcggcc tcgtccgcga tcacgatcgc ctccgcctcg
     3001 gggagggtct ccgcctcggc cggcgccatc tcgggccgga acgccaggcc gacttccggg
     3061 accatgaggt cgaccgcccc ctcgccgatc tctcggccgc cgggctcggc gacgacgagc
     3121 ttcagccggt agttgccggg agcgatcggg cggaggaacg aatacgtgaa cggctttccg
     3181 tccatgacgt cacccgagac gggatagcgg aacgcctgga ccatctcgtc tccgcgcgag
     3241 aaggtgcccc ggagctcgcc ggagaacgcg cggatcccct tggcggctgc ggctttggaa
     3301 agctcggccg aggcaaggcg gatccggacg acggtcttgc ccgccgccat cccgaggtac
     3361 tccgcggtga cctccagcgg gaaacgaccc gccggggtct tcaactcggt cttgcgggtc
     3421 tgcgccggca gcgagagcgc ggcgagagcg agaaacgtcg ccatgaagcg gatctttggg
     3481 agcatttcgc agttccgaac cggagagcgg ggcggaagca ttcccgaggt tggtcgcctt
     3541 aaggcgacgc ttacgcggtc gcggcgagcg gggagccctc gaagaaccgg acgacggcct
     3601 cccggaagtc cgccggcgtc tcaagctcgg agatccgcac ccgcagcgcc cgcccgttcg
     3661 gcagcccgtg cgtgtaccac ccggtcatgg tcttgagctt gtgcagcgcc tcccgcgcgt
     3721 cccgggcggt cgcctcgatc tgcccgaagt gccagagcat caggtcgcgg cgttcggccg
     3781 cggtcgcttc ccgcggcgtt cctccggcga ggcggtcggc ggcctggcgg aagatccacg
     3841 gattcttcat cgtcgcgcgg ccgatcatca cgccgtcaca ccccgtatcg cggagcatcg
     3901 ccaggacgtc gtcggcggtc tcgacgtcgc cgtttccgat caccgggatc gaaagcgcgc
     3961 gtttcaactg cgcgatctct ccccggtccg cgttccccgc gtacatctgc cgcgccgtgc
     4021 gcgcgtgcat cgcgacggcg gcggccccct cgtcctcgca gatgcgcccg aggtcgagga
     4081 agttcttccg cgggtcgtcg agtcccagcc ggaacttcac cgtcagcggg atcgagagcc
     4141 ggctcttcac ctcgcgcacg attttccggg cgagcgggag gtcacccatg agcgcggcgc
     4201 ccgcgcatcc cttcaggacc ttgttcgcgg gacatcccat gttgatgtcg cagacgtccg
     4261 gcgcgagctc ttccacgatc tccgccgcgg cggccatccg gtcggcgttc gacccgtaga
     4321 tctggatcga gagtggtcgc tcctcgtcgg agaaaaacat cttcgcccgg tggcgcgcgt
     4381 tgcctcgcgt gatcgcttcc gaggagatga actccatcgt gacgaggccg acgcctccga
     4441 tccgccggag catgagccga tagaggtggt ccgtgatccc tgccatcggg gcgaggacga
     4501 ggggcgggtc cacggtgacg ctgccgagtc gaatcatcga actcgtcatt ctaccgacct
     4561 cgccttgaat taagattcgc ccgcgaccag cggagggttc tcgccatggc ggtgtccggt
     4621 gccacgattt caggcggtgc gagggaaagt ctctggatcc gtagccgtag gttcgacctg
     4681 cttttcctga cgctctccgg ggtgctggtc ttcctgccgt acctttccta cggcctcctc
     4741 cagcgcctcg gagcgtctcc cgggacggcg agcctgatcg tcggcctcac ggtcacgctc
     4801 ctggtgggcg gaccgcacat gtactcgacc taccttcgca ccgcgctcga gccgcgcttt
     4861 cgcgctcgct acggattcct cgcctacctt ccgctgctgc tgatccccgt gctcgtcata
     4921 ctcggcgcgc tgcacgcttt cctgctcctc atcacggcat ttttcttctg ggcctccctc
     4981 cacgtcacgc aacaggcgca gtggatcgcg gagacctatc gccggaaggc gaccggccgc
     5041 gtctcgatgg tggatcgact gctcgacggc gcggtcatac tcggcgccct ctacacgatg
     5101 gcgatgtaca agttcgtgga gggccgcttc gctctcggcg acagcacgct gctcttcccc
     5161 gagtttctca aacggcgcga gatcgcgatc gctttcacgg tcggcttcgc cgcgttgttc
     5221 ctcttctacg tcttccggac ggccgcggag atccgacggg gcgaagcgag cgttccacgc
     5281 ctcctcttca tggcgttcac cgtcgggctc gcgttcgtcg tcccgatctt cgacaacctc
     5341 gacgtcgcgt tccagggctt caacacgtgg cactccctcc agtacctggc gctgacgtgg
     5401 ttcatccttt cgcgcaacgc ggagcgcggt gcgatcggga gctcactcgc gcagaaactc
     5461 gccggaaccg agaaaaccgg aaggttctac ggcgccatga tcctcgcgac ggtgatggcc
     5521 ggggggatct atctcgtgct gtggaagggg ctcgggttcc cgcaggacaa gagttactac
     5581 gtggtggtgc tctctttcct cctgatccac tacttctacg accacttcct gttccgggac
     5641 ttcgcgccgc tcgacgcccg ggaaggggcc cgggcctaag tctttttcgt gagctcttcg
     5701 taggagctca ccttcgtgta tcccttcggc ggctcgaaga gcttcgccga cgctcccacc
     5761 cggaggtcga ggagctgcgt gtcggtgaac ggctgatatt cgtcacccgg gtccttcttg
     5821 gcgtgctcga tcctcacgac cagccggtcg aggtcgcgcg ccacccagat cgtcgcgacg
     5881 tcccccgacg gcagcgcgag gcgccagcgc tcgcaggggt ggaggccgtg ggtcgcgtcg
     5941 tcctgccgct ccgcctttcc gccgaaccga tcggcgtagg ccgcgacgtc gaacttcgac
     6001 tgcaggggat agccgggcac cgcctcgtcg gtcggctccc agcgggtctc gtagatcttc
     6061 ttcgtcgagg ggcggagctg ccagaccttg tggagatccg gccgcacgat cttgacgatc
     6121 tcggagccgt ccacgttacc cgagatccag acgctctcgc cgcggaccgc gatgcggaag
     6181 cggtcctccg aggaataaac gagaaccgcg cgatagccat ctcgcgaaac cgtcttcgga
     6241 ccgccgcagg cgatcgcgag aaggatcgcc gccgcggcgg cggaatggag gaagagcctc
     6301 ggcgacatcg gcacgctgca attctccttc gcgtcggcgg cgcgcgcgag ataatcccgc
     6361 gcgtgccgtg gtggaaaact ggttcggcga agagtatctc gggctgaact gacagcgccg
     6421 tgagcacgat accgctcgaa aagtacccgg ggcttcccgc gctcttcgtc gacttcgcgc
     6481 ggggacgaag cgggctccat ccggatccgc cgactctcga tgttgcggtc gcccggggcc
     6541 gggagctcct ctcacgtcga gacgcgccgc gcctgcccgt gagcgcgttc cgtcaccggt
     6601 cgccggcggg ccctgccgcc gcggagagtc tcgcgtcggg ccgcgccgtt gcggtgctgg
     6661 cgggacacca gatcggcctc ttcaccggcc cgctcttcac gatcgtgaag gccctcgacg
     6721 ccgtcgcgtt cgcgcgcgag ctctcccggc gcggcgtccc ggccgttgcc gtcttctggg
     6781 cgctcaccga cgaccacgat ctcgaggaga tcgcccgaac cgcccgaccg ggacccgacg
     6841 gtccggacaa gttcctgctc gagggcgcgg atcggtccaa ccgtcgcccc gtgggcgcgc
     6901 tcaggatccc cgaaaagatc gcgggggtcg tcgaggcgtt ccgtgccgac gcgcgcggac
     6961 cggaagcgct ggagatcctc gacgggttcg cgaggcgcta cgccgcgggt cgcacctatg
     7021 ccgaggcttt cgtcgacgcg ctcttcgacc tgaccgggga cgacccggtc ctcgtcgtcg
     7081 atcccctggg gccggaggtg gcggaggcgg cgcggcactt ctttctcgcc gccgcggagc
     7141 ggcgcgacga ggtcgcccgc atcctcgccg gcggcgccca tcgggtcgcg gaggccggac
     7201 gcgagccgcc cgtgccgtac cgcgccgacc tcttcccgtt cttcatcgtg gaagaaggca
     7261 gccggcgccg cgtgcgcgcc ggcgagatcg acgaggccgc aaaaaaggtc gagcgcggag
     7321 aagcccgcgt ctccgccgac gtactcacgc ggcccgttct gaagtctctc gtgctgcccg
     7381 cggcggtctc cgtcctcggc ccgagcgaga tcgcctacca cgcccagtcg ctcccgctct
     7441 tccctctctt cggcgcgaac ccgccggtgc tgatgccccg gtcgctcctc gtccttcgcg
     7501 gtccggcgga acgccgcgcg cagcaggcgc tcggggtgac cgacgaggac cttctgacgc
     7561 cgggcgccgc gagggcgagc gcggggccgg tgccgcaggc cgaacaggtc gcggaaatcg
     7621 cccggcggct cgatgccgag ctcgcgtcgg tcggctcgga agtcggcgga atcgatccga
     7681 ctttgaccgg agcgctcgaa accgcccgcc ggaaagcagc gtaccagttg gagcagctcg
     7741 ccgagcgcgt tcgaaaggct gccgagcgaa aggacgaggt caccctgcag cgacggagaa
     7801 ggctcgagac gatggtcctt ccgggcggag atgcggccga acgcgtctat ccgccgctcg
     7861 tgttcctgct cgcgtggggg cgcacgctcc tcgacaccct gcggggagcg gccggacgcg
     7921 gggcggcgga cgcggcgatc gtcgacgtcg aaacagcggc tggcgccggg gcggagatga
     7981 cgagaacgac tcatgccggt tgacgtcctc gcgatcgcgg cgcaccccga cgacgtcgag
     8041 atgacgtgcg ggggaaccct cttgcggctg aaggagcgcg ggaagaagtt cgggatcgtc
     8101 gacctcaccg ccggcgagat gggcacgcgg ggaacctccg agacgcggcg gctcgaggcg
     8161 gagcgcgccg cggaaatcct cggcgccgag ttccgcgaga cgctcgactt cggcgacggc
     8221 ggcctcgtca cctcccgaga gcacgagctc gttctcatgg acgtgatccg ccgcgagaag
     8281 ccgcgcctca tcctgacgtc gtatcccgac gaccggcacc cggatcattc gcgcgcgggc
     8341 cggttcgtca ccgatgccgc cttttacgcc ggactgcgca aggtcgagac gaaccatccg
     8401 gcgcaccgtc cgcaacacac gatctacttc cccaccgccg tcatgcacga gccggatttc
     8461 gttgtggaca tcacggcggt gatggagaca cgacgtgccg caatcctcgc gttcgcgagc
     8521 cagttccacc gcgaggggtc gacggagccc gagacgatgc tctcccggaa gatgtttctc
     8581 gaggcgctcg acgcgcgggc gcggcacgtc ggatttctcg ggggcgtcga gttcggggaa
     8641 gggtttctct cgaaacggcc gcctcgaatc gacgacctga tcggcgcgtt cgagggactc
     8701 gagccgggct tctgagcccg cgcggacccc tcgcgtgaag atcggcatca cctgctatcc
     8761 gacctacggc gggagcggtg tcgtcgcgac ggagctcggg ctcgcactcg ccgaccgcgg
     8821 cgacgagatc cacttcatct cctacgatct cccgtcccgg ctcgccctcc cccgtccgcg
     8881 cgtctacttc cacgaggtcg tcgcgccgac gtatcccctg ttcgtatcgc caccgtacac
     8941 gctcgccctg gcgacgaaga tggcggaggt cgccgagcac gtgcgcctcg acctcctcca
     9001 cgtccactac gcgctccctc acgcgatttc cgcgattctc gcgcgcgaga tgagcgacgg
     9061 gaacggcgtc cggctgaagg tcgtgacgac gctgcacggc accgacatca cgatcgtcgg
     9121 acaggaccgc tcctatctgc ccatcacccg gtgggggatc gagcagagcg acgccgtcac
     9181 cgcggtgtcg cgctacttga cggacatgac gctccgcgag ctcggcgtcc gccggacggt
     9241 cgagacgatc ccgaatttcg tggatccgaa tcgctaccgc cccgacggcg cgtccgccta
     9301 cgcgcgaacc ctcgtgagag acgacgaggt cggagtgatc cacgtctcca atttccggcc
     9361 ggtcaagcga atccccgacg tgctcgcggt gttcgaccgc atccgccggg aggttcccgc
     9421 tcgcctcctc ctgatcggag acggtccgga gcgctcgctc gcggagcgca tcgcgaggca
     9481 gcgcggtttc gaggaccgcg cgacgttcct cggcaacgtg gccgcgatcg aaacaatttt
     9541 tccggtggcg aaggtgatgc tgcttccctc cgacgccgag tcgttcggtc ttgccgcgct
     9601 cgaggcgatg gcgtgtggag tgccggtcgt cgggactgcg gccgggggcc tgcccgaagt
     9661 cgtcgcggac ggcgcctgcg gctacctgcg tcccgtgggc gacgtcgaag ggatggccga
     9721 ggccgcgctc acgctgctgc gggacccgga aaagtggcac cggttttcgt cggaggcgcg
     9781 ccgccgcgcg gtcgaggagt ttccgaccga cgagatcgtc tcgcgctatc ggaaactcta
     9841 cgaggcgacg ctcggctgag gaggcccttc ccggcgcggc cggcgggtcc cgagctccac
     9901 gagcgcacgt tcccggtcgg ccggcggaca aaaggaggcg cgccaggagt tctcggcgag
     9961 gaggcgcatc gtgcggtcgc cgaacccgaa cctctcccgc acgagcgcgt gctcctgcgc
    10021 ggtcgtggtc ccgaagagaa gaggatcgtc cgcgccgatc gcgacgcgga cgccggcgtc
    10081 gaggaggcgc ggcaacggat ggtccccgag gtcctggacg gcgccggtga cgacgttcga
    10141 cgtcggcgag acccagaggg gcgtcgactc ctccgcgaac ctcgcgagca gatgcgcgtc
    10201 gttcgcggcg gcgattccgt ggtccacccg gtcgggccgc agg
//
LOCUS       DASWAV010000008         2099 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U2665
            k141_280465, whole genome shotgun sequence.
ACCESSION   DASWAV010000008 DASWAV010000000
VERSION     DASWAV010000008.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960316
            Sequence Read Archive: SRR10260007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2099)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2099)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:47:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,929
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2099
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_280465"
                     /isolate="SMAG_U2665"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.36444 N 132.31889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..340
                     /locus_tag="VF958_00175"
     CDS             <1..340
                     /locus_tag="VF958_00175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9147549.1"
                     /translation="FRHLEEGWVDTPEPRREQRRRRRRGRGGERGTGAAATPDKAGGR
                     PRGAPPPGRERAPRPPKPALSPTEPAPSQAATGSTAAGARPEAHRRFRRRRRRGRGSG
                     GGNAPPAPTA"
     gene            337..1485
                     /locus_tag="VF958_00180"
     CDS             337..1485
                     /locus_tag="VF958_00180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012259012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEX9147550.1"
                     /translation="MNFDISEDQARIRNAVREFAEQEIAPGVAERERTETFPREIVGK
                     LGEMGILGMMVPEEYGGSGLDALSYILAVEELARVCASTAVIMSVNNSVFCYPVWKFG
                     SERQKKEILSEVASGRALGAYALTEPQSGSDAANQKTVARREGNEYVVNGEKSWITNA
                     GQARWYLVMAMTDPTQGTHGITGFLVSADDPGVTVGAPEAKMGLRASKTAGISLDGVR
                     VPEWRRLGEEGQGFLIAMRTLDHSRIGIAAQGIGIARASYEASVDYARTRETFGRKIA
                     EHEAIAFQIADMRVQIEAARWLTYRAAVKSDTPGVRFSKESSMAKVFATEMANTVAAR
                     AVQIFGGYGYSREYPVERYYRDARVTTIYEGTSEIQRIVISKNILAGR"
     gene            1482..1889
                     /locus_tag="VF958_00185"
     CDS             1482..1889
                     /locus_tag="VF958_00185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9147551.1"
                     /translation="MSDKDNDGRAPEAPAPAPATAPATPGSRSEAASPTGRRPTRPSL
                     SIERTPVSRDHSGYVFRLDPPDLANLRELPGARGKTDEELGTEFLATHGDRLVAALAD
                     DLPAPATIRVLVDPYSRQAFLAYQDRVRGIVSF"
BASE COUNT          339 a          743 c          723 g          294 t
ORIGIN      
        1 gtttcgacac ctcgaggagg ggtgggtgga cacgcctgaa ccgcgtcgtg agcagcgccg
       61 gcggcgccgg cgcggacgcg gaggagagcg ggggaccggg gccgcggcga cgcccgacaa
      121 ggcgggaggc cgcccgcggg gagcaccccc tccgggaagg gagcgggcgc cccgcccgcc
      181 gaagcccgcg ctctctccca ccgagccggc cccgtcccag gcggcgaccg gatcgacggc
      241 cgccggcgcc cggcccgagg cccatcgccg gtttcgccgt cgccgccgcc gcggccgcgg
      301 ctcgggaggc ggaaacgccc cgcccgcccc gaccgcatga acttcgacat ctccgaggac
      361 caggcccgca tccgcaacgc ggttcgcgag ttcgccgagc aggagatcgc tccgggcgtc
      421 gccgagcgcg agcggaccga gacgttccca cgcgaaatcg tcggcaagct cggcgagatg
      481 ggaatcctcg ggatgatggt gcccgaggag tacggaggct ccgggctcga cgccctctcg
      541 tacattctcg ccgtcgaaga gctcgcgcgc gtctgcgcct cgacggcagt gatcatgtcc
      601 gtcaacaact ccgtcttctg ttacccggtc tggaagttcg gctcggagcg gcagaagaaa
      661 gaaatcctct cggaagtcgc ctcggggcgc gcgcttgggg cctacgcgct caccgagccg
      721 cagtcgggct cggacgccgc gaaccagaag accgtcgctc gccgcgaagg aaacgaatac
      781 gtcgtgaacg gcgagaagtc ctggatcacg aacgccggcc aggcacgctg gtacctggtc
      841 atggcgatga cggatccgac gcagggcacc cacggcatca cggggtttct cgtctccgcg
      901 gacgacccgg gcgtgaccgt cggcgctccc gaggcgaaga tggggctgcg cgcgtcgaag
      961 accgcgggaa tctcgctcga cggagtgcgc gtccccgaat ggcggcgact cggcgaggag
     1021 ggacagggat ttctcatcgc gatgagaacc ctggaccatt cgcgcatcgg aatcgccgcg
     1081 caggggatcg ggatcgcccg ggcatcgtac gaggcctccg tcgactacgc gcggacgcgg
     1141 gagacgtttg gccggaagat cgccgagcac gaggcaatcg cgttccagat cgccgacatg
     1201 agggtccaga tcgaagccgc tcgctggctc acctaccgcg ccgcggtgaa gtccgacacc
     1261 ccgggagtgc gattctcgaa agagtcgtcc atggcgaagg ttttcgcgac ggaaatggcg
     1321 aacaccgtgg ccgcccgcgc ggtgcagatc ttcggtggtt acggctactc gcgcgagtac
     1381 ccggtggaac ggtattaccg ggacgcgcgc gtgacgacga tctacgaagg tacgagcgag
     1441 atccagcgca tcgtgatctc gaaaaacatc ctggcgggaa ggtgagcgac aaagacaacg
     1501 acggtcgagc gccggaagcg cccgccccgg cgcccgctac ggcgcccgcc acgccggggt
     1561 cgcggtcgga ggccgcctca cccacgggcc gccggcccac ccgtccgagc ctctcgatcg
     1621 agcggactcc ggtctctcgc gatcattccg gatacgtgtt ccgacttgat ccgcccgatc
     1681 tcgccaacct ccgcgagctt ccgggtgcgc gcggaaagac cgacgaggaa ctcggcacgg
     1741 agttcctggc gacgcacggt gatcgcctgg tggcggcgct cgccgacgac ctgcccgcgc
     1801 ccgcgaccat ccgggttctc gtcgatccgt actcgcgcca ggcgtttctc gcctaccagg
     1861 atcgcgtccg gggaatcgtc tccttctagc cgtcgcggtc gcttgctcgg gatctggccg
     1921 cgccactggc gctcggctcg gcaagccagc ctcgaccgag cgcctgatcc ctcgctgcgc
     1981 tctcgctccc gcgtcgcgag gctcctccct cgattacctc gggagactcc tcgcgccgcg
     2041 aatgggtctc cgcgccccgg cttggtaaga gggccgtcgt cgccagcttc gtcagcccc
//
LOCUS       DASWAV010000009         4758 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U2665
            k141_660, whole genome shotgun sequence.
ACCESSION   DASWAV010000009 DASWAV010000000
VERSION     DASWAV010000009.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960316
            Sequence Read Archive: SRR10260007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 4758)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4758)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:47:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,929
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4758
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_660"
                     /isolate="SMAG_U2665"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.36444 N 132.31889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1402
                     /locus_tag="VF958_00190"
     CDS             <1..1402
                     /locus_tag="VF958_00190"
                     /inference="COORDINATES: protein motif:HMM:NF026141.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HEX9147552.1"
                     /translation="EGRVAPVKGFGPKTQERILRGIEMLRTTSGLHLYSAVRWRADEL
                     VAAIEQTGLATRVAIAGSLRRRREVVGDINIVAASTRKAELARAFPAVAPGVVEVVAR
                     GDTRVSLGLAEGIVADLRVVSEREFPAALLDFTGSKEHNTALGALATERGWKLSESGF
                     SGGHGGEGEFPSVQSEEEIYARLGLQFVPPELREGADEIEAAARGQIPRLVEDSDVRG
                     LIHVHTTESDGRATLEEMVSATRDKGYEWVAITDHSPTAAYAGGLTPERVRRQRLAID
                     AIRSRFPGFRIFHGTEADILADGSIDFGDEFLGVFDVVVASVHSRFGLPRDEQTKRLI
                     RAVENPRVSVLGHPTGRLLLTRKGVDADVDAVLAAARRSGCAVEVNGSPHRLDLDWRL
                     AGDAARSGMTFALGPDAHSIRELDNTAYAVGIARKGWVTAPATLNAKTAGELEAWLAK
                     RRGAPLSSPGAFPNPD"
     gene            1428..2075
                     /locus_tag="VF958_00195"
     CDS             1428..2075
                     /locus_tag="VF958_00195"
                     /inference="COORDINATES: protein motif:HMM:NF025605.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEX9147553.1"
                     /translation="MGRPAKASRDAVLRAARDAFAERGFDGTTLSDISARIGLSPSAI
                     LRHAPTKEALFSAAMSSPADDVPRMEFVAEAGGEEDPADVLRRVAFAVVPFIESKLGE
                     NIARWFRAKTADEARTIKLPFDPRKRPSPPARALALLEDYFRRASRAGRLQVRDPRAA
                     ALAYLGSLNAYVFLHRVMRIVDPPVPLPRYVDTLLEIWKRGAIRPARRAARRKSP"
     gene            2072..3007
                     /locus_tag="VF958_00200"
     CDS             2072..3007
                     /locus_tag="VF958_00200"
                     /inference="COORDINATES: protein motif:HMM:NF027890.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family efflux transporter periplasmic
                     adaptor subunit"
                     /protein_id="HEX9147554.1"
                     /translation="MKKRRLAIIVLLAVAVGGAFWAFRYSRRDKPLVLSGSIEARDVE
                     VGSLVGGRVSAVLVPEGATVHAGQPLVTFETDLGQLQIREQRAVVDQARATLAKMRAG
                     PRSEEIVRARAEAENAERERSRLKALSDGGIIGRQQYEDAATRAKTAQETYRELARGN
                     RPEDVRAAEAALDREEQRLAFLLRQSEETVVKSPADGIVESLDLRPGDLVPANQPVAR
                     ILEPGQLWVRVWVPEPLLGGVHVGQKVAVTVDTFPGREFPGKIVEIRQQGEYTPRNIQ
                     TLKQRMDQVFGAKVALEPSPELKPGMAAQVRLLES"
     gene            3014..4015
                     /locus_tag="VF958_00205"
     CDS             3014..4015
                     /locus_tag="VF958_00205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007415491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX9147555.1"
                     /translation="MAENSNGIEVHDVSRDFGKVRALDRVSLAVPRGEIYGLLGPNGS
                     GKSTMIRILCGLLAPTEGTARVDGLDVATQGEEIRRRIGYVPQRFSLYEDLTVMENLT
                     FFAEVYGLERGRLKERRDWAIGLTSIGPYVDRLAAQLSGGWKQRLALAASLMHEPRVL
                     FLDEPTAGIDPVARRELWNLLFTLAAQGVTLLVSTHYMDEAERCGRVGYLYLSRMLVE
                     GDPKDLTRLPEVTPSGTRRVEAECEAGVAAVMQHARELPYVEDLTIFGNSLHILMRAE
                     IADGRIVRDLEAASGSPVKVRPIDASLEDVFVRLTEIEKAKRGEVGVETAPFPVTAA"
     gene            4012..>4758
                     /locus_tag="VF958_00210"
     CDS             4012..>4758
                     /locus_tag="VF958_00210"
                     /inference="COORDINATES: protein motif:HMM:NF024091.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX9147556.1"
                     /translation="MTRGFRAVLRKEAKQMLRDRGTLQFALMVPAMQLVLFGLIDTNV
                     RHVPTAVFDQSRTQESRQVVTDLVNTGLFDVLEYVPSRDDLRERIVSGRASVGVEIPP
                     DYARRRLAGRPADVLVLIDGSDSSISSQALAAANGVALAHSLQELGAGAGFESLSIRV
                     HPLLLFNPDSRSANLLIPGLVGILLTFSGTLLAAFAIVREKERGTLEQLMVTPASPVA
                     VVLGKLIPYLVLAFLQLLLVLVLMTTVFRVP"
BASE COUNT          715 a         1670 c         1633 g          740 t
ORIGIN      
        1 cgaggggcgc gtcgcgccgg tcaagggttt cggaccgaag acgcaggagc ggatcctccg
       61 gggaatcgag atgctccgga cgacctcggg acttcacctc tactcggccg tgcgctggcg
      121 cgccgacgag ctcgtcgcgg cgatcgagca gacgggtctc gccacgcggg tcgccatcgc
      181 cgggagcctc cggcggcggc gagaggtcgt cggtgacatc aacatcgtcg ccgcttcgac
      241 gcgcaaagcc gagctcgcgc gcgcattccc ggcggtcgcg cccggcgtcg tggaggtcgt
      301 ggcccgggga gacacgcgag tgtcgctcgg gctcgccgaa ggaatcgtgg cggacctgcg
      361 tgtcgtctcg gagcgggagt tcccggctgc gctcctcgac ttcacgggat cgaaggagca
      421 caacacggct ctcggcgcgc tggcgacgga acggggttgg aagctgagcg agtccggctt
      481 ctccggcgga cacggcgggg agggggagtt ccccagcgtc cagagcgagg aggagatcta
      541 tgcccgtctc gggcttcagt tcgttccgcc cgagctgcgc gagggggcgg atgagatcga
      601 agcagccgcc cgaggtcaga tccctcggct ggtggaggac tccgacgtcc gtgggctgat
      661 tcacgtccat acgaccgagt ccgacggtcg agccacgctc gaggagatgg tctcggccac
      721 ccgcgacaag ggctacgagt gggtcgccat caccgatcac agcccgacgg ccgcctacgc
      781 cggcggcctg acgccggagc gtgttcgccg gcaacggctg gcgatcgacg cgatccgatc
      841 gcggtttccc gggttccgga tctttcacgg aacggaggcc gacatcctcg cggacggttc
      901 gatcgacttc ggcgacgagt tcctcggggt cttcgacgtc gtcgtcgcgt cggttcactc
      961 gcgattcggg ctgccccgcg acgagcagac gaagcggttg atccgcgccg tcgagaatcc
     1021 ccgcgtctcg gtgctcggcc atccgaccgg ccggctgctc ctgacccgca agggcgtcga
     1081 cgccgacgtg gacgcagtgc tcgccgcggc ccggcggtcc gggtgcgccg tcgaggtcaa
     1141 cgggtcgccc caccggctcg acctcgactg gaggctcgcc ggggacgccg cacgaagtgg
     1201 gatgaccttc gccctggggc cggacgctca ctcgatccgc gagctcgaca acacggccta
     1261 cgcagtcggt atcgcccgga aaggatgggt caccgctccg gcgaccttga acgcgaagac
     1321 cgccggcgag ctcgaggcct ggctggcgaa gcgccggggc gcgccgctct ccagtcccgg
     1381 ggcgttcccg aacccagatt agtaaatgag cgtatactta tttacctatg ggccgccctg
     1441 cgaaagccag ccgtgacgcc gttctccgcg cggcccggga cgcgttcgcc gagcgcgggt
     1501 tcgacggcac gaccctctcc gacatctcgg cgcgcatcgg cctctccccc tccgcgatcc
     1561 tgcggcacgc gccgacgaag gaggctctct tctcggcggc aatgtcgagc ccggccgacg
     1621 acgttccccg catggagttc gtggccgagg cgggcggcga agaagaccct gccgacgttc
     1681 tccggcgggt cgcgttcgcc gtcgttccgt tcatcgagtc gaagctcggc gagaacatcg
     1741 cgcggtggtt ccgggcgaag acggcggacg aagctcgaac gatcaaactc cccttcgacc
     1801 ctcggaagcg gccgagcccg cccgcccgcg ccctcgctct tctggaagat tactttcggc
     1861 gggcgtcgcg cgcgggcagg ctgcaggtcc gcgacccccg tgcggccgca ctcgcctacc
     1921 tcggatcgct gaacgcctat gtttttctgc atcgcgtgat gcggatcgtc gacccgcccg
     1981 tgcccctccc ccgttacgtg gacacgctgc tcgagatctg gaagcggggc gcgatccggc
     2041 ccgcccggcg cgccgcgagg agaaagtcac cttgaagaag cgccgcctcg cgatcatcgt
     2101 cctgctggcg gtcgccgtcg gaggcgcgtt ctgggctttc cgctattcgc gccgggacaa
     2161 gccgctcgtc ctgtccgggt cgatcgaggc gcgcgacgtc gaggtcggct ccctggtcgg
     2221 aggccgcgtc tcggcggtcc tcgtccccga aggggcgaca gtccacgccg gccagcccct
     2281 cgtcacgttc gagacggacc tcgggcagct ccagatccgc gagcagcgcg cggtggtcga
     2341 ccaggcccgg gccaccctcg cgaagatgcg cgcgggtccc cgcagcgagg agatcgtgcg
     2401 cgcgcgcgcc gaggcggaaa acgcggagcg cgaacgcagc cgcttgaagg cgctctccga
     2461 cggcggcatc atcggtcgcc agcaatacga ggacgccgcg acccgcgcga agacggcgca
     2521 ggaaacgtac cgggaactcg cccgcggcaa ccggccggaa gacgtccgcg cggccgaggc
     2581 ggctctcgac cgcgaggagc agcgccttgc atttctcctc cgccagagtg aggagaccgt
     2641 cgtcaagtct cccgctgacg ggatcgtcga atcgctcgac ctgcgcccgg gagacctcgt
     2701 gccggcgaac cagccggtcg cccggattct tgagcccggc cagctctggg tccgggtgtg
     2761 ggtgcccgag ccgttgctcg gcggcgtcca cgtggggcag aaggtcgcgg tcacggtcga
     2821 caccttcccg gggcgcgagt tccccggaaa gatcgtcgag atccggcagc aaggcgagta
     2881 cacgccccgc aacatacaga ccctgaagca gcggatggac caggtcttcg gagcgaaggt
     2941 cgcgctggag ccctccccgg agctgaagcc cgggatggcg gcgcaggtgc ggcttctcga
     3001 gagctgagaa caggtggccg agaactcgaa cggcatcgaa gtccacgacg tctcccgcga
     3061 cttcgggaaa gtgcgcgctc tcgatcgggt gtcgctcgcc gtcccccgcg gcgagatcta
     3121 cgggctcctc ggtccgaacg gctcaggcaa gtccacgatg atccgcatcc tctgcggcct
     3181 tctcgcgccg acggagggaa cggcccgcgt cgacgggctc gacgtcgcga cgcaggggga
     3241 agagatccgg aggcggatcg gctacgtccc ccagaggttc tccctctacg aagacctgac
     3301 cgtaatggag aacctgacgt ttttcgcgga ggtctatggc ctcgagcgag ggcggctgaa
     3361 ggagcggcgc gactgggcga tcgggctcac gtcgatcgga ccctacgtcg accggctcgc
     3421 cgcgcagctc tccggcggct ggaagcagcg cctcgcgctc gcggcctcgc tgatgcacga
     3481 gccgcgggtg ctgttcttgg acgagcccac ggccgggatc gacccggtcg cgcgccgcga
     3541 gctctggaac ctcctcttca ccctcgccgc gcagggagtc accctcctcg tcagcacgca
     3601 ttacatggac gaggcggagc gctgcggacg cgtcggatac ctgtacctct ctcgcatgct
     3661 cgtcgaagga gatccgaagg acctgacgcg cctccccgag gtcacgccgt cgggaacccg
     3721 gcgcgtggaa gcggagtgcg aggcgggcgt cgcggcggtc atgcagcacg cccgcgagct
     3781 cccctacgtc gaggacctca cgatcttcgg aaactcgctt cacatcctga tgcgcgccga
     3841 gatcgcggac ggccggatcg tgcgcgacct cgaagcggcc tccggatcgc ccgtgaaggt
     3901 ccggcccatc gacgcatctc tcgaggacgt cttcgtgcgc ctgaccgaga tcgagaaggc
     3961 caagcgcggc gaagtcggcg tcgagacggc gccctttccg gtgaccgcgg catgacgcgg
     4021 ggcttccggg cggtgctgcg gaaagaggcg aagcagatgc tccgcgaccg ggggacgctt
     4081 cagttcgcgc tcatggtccc agcaatgcag ctcgtcctgt tcggcctgat cgacactaac
     4141 gtccgccacg tccccacggc cgtcttcgac cagagccgga ctcaggagag ccggcaggtc
     4201 gtcacggacc tggtcaacac cggccttttc gacgtcctcg agtacgttcc ctcccgcgac
     4261 gatctccgcg agcgaatcgt ctccggccgg gcttccgtcg gcgtcgagat cccgcccgac
     4321 tacgcgcgcc gcaggctcgc gggacggccg gcggacgtcc tcgtcctgat cgacggatcg
     4381 gattcgtcga tctcttcgca ggcgctggcc gccgccaacg gagttgccct cgcgcactct
     4441 ctccaggagc tcggcgcggg cgccggcttc gagagcctct cgatccgggt ccatccgctc
     4501 cttctcttca acccggactc gcggagcgcg aacctgctga tcccgggtct cgtcggaatt
     4561 ctgctgacgt tctcggggac gctgctcgcg gcgttcgcca tcgtccgcga gaaggagcgg
     4621 gggacgctcg agcagctcat ggtcacgccg gcgtctcccg tggcggtcgt gctcggcaag
     4681 ctcatcccct acctcgttct cgccttcctc cagcttctcc tcgtcctcgt gctcatgacg
     4741 acggtctttc gcgtcccg
//
LOCUS       DASWAV010000010         4249 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U2665
            k141_40780, whole genome shotgun sequence.
ACCESSION   DASWAV010000010 DASWAV010000000
VERSION     DASWAV010000010.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960316
            Sequence Read Archive: SRR10260007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 4249)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4249)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:47:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,929
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4249
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_40780"
                     /isolate="SMAG_U2665"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.36444 N 132.31889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..585)
                     /locus_tag="VF958_00215"
     CDS             complement(<1..585)
                     /locus_tag="VF958_00215"
                     /inference="COORDINATES: protein motif:HMM:NF014896.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HEX9147557.1"
                     /translation="MIDAARRFRVLLILLALGRPGTAAAQSPAYPATRRDDVVDDYFG
                     TKVPDPYRWLEQLESPDTVDWVKAQSRLTSRYLGSLPGRADIERRLESLWSSARTEIP
                     WQAAGRLFYARNNGLQQQPAFYAQKGLHGAPRAVLDPNKISPDGSIAFRDFSPSADGR
                     LVAFTVARGGADAGDIHVRAVASGRELDDVLRGAG"
     gene            644..1399
                     /locus_tag="VF958_00220"
     CDS             644..1399
                     /locus_tag="VF958_00220"
                     /inference="COORDINATES: protein motif:HMM:NF015297.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase/citrate lyase family protein"
                     /protein_id="HEX9147558.1"
                     /translation="MPDLRRRVTAGESLLGCFLTWPVAGIVELAALAGFDFVVVDVEH
                     GSFSIESVAATVLACDSAGIAAIVRSPTAVSEQLGRYLDAGAAGTLLPRVESVMMARA
                     AVEALKFAPDGKRGLGGVRANRYGTTPLAEFVRRANEETLVAVQIEREGALAELRDIA
                     DLPAVDVLYVGPNDLSQALGAPGDMTSERFLSALARVASEATRAGKASGIMVGNRDQI
                     PALRALGYRFFTTSDRALLLEAGRSWRTALSLH"
     gene            complement(1318..1668)
                     /locus_tag="VF958_00225"
     CDS             complement(1318..1668)
                     /locus_tag="VF958_00225"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: protein motif:HMM:NF013939.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0046417 - chorismate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate mutase"
                     /protein_id="HEX9147559.1"
                     /translation="MSPELRRIRRAIDAVDQLLSAALALRAELVVRAALLPGKRRDRI
                     REAEILAAAARRPTGGLRPAAVRRIFRAIIDAGIELQASLPASQSAQWSERAVLHDRP
                     ASSRSARSLVVKKR"
     gene            complement(1759..2790)
                     /locus_tag="VF958_00230"
     CDS             complement(1759..2790)
                     /locus_tag="VF958_00230"
                     /inference="COORDINATES: protein motif:HMM:NF013316.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase U32 family protein"
                     /protein_id="HEX9147560.1"
                     /translation="MAEPTGNRVLGYTGRQVKEGRMVELLAPAGNLPMVEAVVAGGAD
                     AVYVGPRGWSRRRDQYEMPDDDVRAAIDIAHGGPSKLRVAINTNMQSHEIAPMLRKME
                     RFVSWGVDGAIMTDVGAIAEVHRRFPQLTIHASIGANILNDEDVRFYRSIGVSQVVAD
                     TKLTLKELASRTEQIDVGIEILIHANKCYTYLGKCWMSPYHRLERTTDAFGKDLFKGS
                     PNRGGLDYRVCLESWELYSGDEMWEKSVALKNDAFFLFEDIPDLIDLPVQCLKVQGRE
                     YPVPLVERMVRFYRDLIDACVASPKGERFDLAPWKSRLAAVQMERDMVRSDGTRHLLA
                     EARQPVPAH"
     gene            complement(2804..3778)
                     /locus_tag="VF958_00235"
     CDS             complement(2804..3778)
                     /locus_tag="VF958_00235"
                     /inference="COORDINATES: protein motif:HMM:NF013316.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase U32 family protein"
                     /protein_id="HEX9147561.1"
                     /translation="MNREQEAMSRFELNTTIANLRDLRESDLRDYDAVYLGDIYCRIY
                     EANFLEQLGDLREGLRILQDQGKRACVTTYAAPRNDFLPKVRRMLEASVEAGAQAVEV
                     HNLGILKIVHDEFPQLPVHVGGFANVYTAAGVEVLRDFGAVRFTPNYELSLDEINEIT
                     TACGAAMELLVHGKMPLGISDYCFLLEYEEKWGVKCPDLCQKELFLKQGDWAMKSVGK
                     GILSGKDVCMLEHLPRLIAEGHRHFRIETVSETPEYRREVAAVYRAALDRAAAGDADL
                     DGSWWETLGRHSRIGLCNGFYFGKSGMDYAGIRPESIGSLLTISRAES"
     gene            complement(3788..>4249)
                     /locus_tag="VF958_00240"
     CDS             complement(3788..>4249)
                     /locus_tag="VF958_00240"
                     /inference="COORDINATES: protein motif:HMM:NF013316.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HEX9147562.1"
                     /translation="HMVEEFVLLGKCWMPSYVHLKPSLRPEVLRLETAADADATESVR
                     RGLRQTGSMKRGGVGACYRICQQPWELYASGRRMDARLFPSRQISRIAEVHAYAEAGV
                     DVLKLQGRSLPVELLAPLVRRYREAIDARTAPPASAVGFAELPAAWAVVGR"
BASE COUNT          684 a         1444 c         1371 g          750 t
ORIGIN      
        1 tcccgccccg cgcaggacgt cgtcgagctc gcgcccgctg gcgaccgcac gcacgtggat
       61 gtccccggcg tcggccccgc cgcgggcgac ggtaaaggcg accaggcgtc cgtccgcgga
      121 tggcgaaaag tcccgaaagg cgatcgatcc gtccggggaa atcttgttcg ggtcgagcac
      181 ggcgcgtggc gctccgtgaa gccccttctg cgcgtagaaa gcgggttgct gctggaggcc
      241 gttgttcctc gcgtaaaaga gcctgcccgc agcctgccag gggatctcgg tgcgcgcgga
      301 actccagagc gactcgagac gccgctcgat gtcggctcga ccgggaaggc tcccgaggta
      361 ccgggacgtg agtctgctct gcgccttcac ccagtcgacc gtatcggggc tctcgagctg
      421 ctcgagccag cgataggggt cagggacttt cgtgccgaag tagtcgtcga cgacgtcatc
      481 gcgacgggtg gcggggtatg ccggggattg cgcggcggcc gtccccggac gtccgagcgc
      541 caggagaatc agcaggacac ggaaccgccg ggcggcgtcg atcacgactc ctctcctttc
      601 cgaatcagaa gtcctcgggc ggaacagctg ataagtttcg cagatgcccg atctgcgccg
      661 cagggtaacc gccggagaat ctctcctcgg ttgtttcctc acctggccgg ttgcgggcat
      721 cgtcgagctg gcagctctgg cgggcttcga cttcgtcgtc gtggatgtcg agcatggttc
      781 cttttcgatc gagtcggtcg cggcgacggt tctcgcctgc gattcggcgg ggatcgcggc
      841 gatcgtgcgc tccccgacgg cggtgtcgga gcagctcggc cggtatctcg acgccggcgc
      901 ggccgggacg ctcctcccgc gcgtcgagag cgtcatgatg gcacgtgctg ctgtcgaggc
      961 gctgaagttc gccccggacg ggaagcgcgg cctgggcgga gtccgcgcca accgttatgg
     1021 cacgacgccg ctcgcggagt tcgtccgccg tgcgaacgag gagacgctcg tcgccgttca
     1081 gatcgagcgc gagggcgcgc tggcggaact ccgtgacatc gccgatctgc ctgcggttga
     1141 cgtgctctac gtcggaccga acgacctctc gcaggcgctc ggggcgccgg gcgacatgac
     1201 ctccgagcgc ttcctctccg ctctcgcgcg ggtcgcgtcg gaggcgacgc gtgccggaaa
     1261 ggcctccggg atcatggtgg gcaaccggga ccagatcccg gcgcttcggg cgctcggcta
     1321 ccgcttcttc acgacgagcg atcgagcgct cctgctcgag gccggacgat cgtggaggac
     1381 ggccctctcg ctccactgag ccgactgact tgccgggagt gaggcctgga gctcgatccc
     1441 ggcgtcgatg atcgcccgga atatgcgccg gacggcggcg gggcgaaggc ctcccgtcgg
     1501 acgccgcgct gcggccgcga ggatctcggc ctcgcggatc cggtctctgc gcttgccggg
     1561 caggagcgcc gcgcgcacga cgagctccgc gcggagcgcg agggcggcag agagaagctg
     1621 atccaccgcg tcgatcgcgc ggcggatccg acgaagctcg gggctcacgg ggatctcttt
     1681 tcggcggcgt gagctcaaag ccgcactcgc cttcgcactc caagactccg gatgcgggcc
     1741 cgaagtcctg ccatcctcct agtgggccgg caccggctgc cgcgcctccg cgagcagatg
     1801 gcgggtcccg tcggaccgca ccatgtcccg ctccatctgg acggcggcca ggcgtgactt
     1861 ccagggcgca aggtcgaagc gctcgccctt cggcgaagcg acgcaggcgt cgatcaaatc
     1921 ccggtagaac cgtaccatcc gctcgacgag cggcacgggg tactcgcgcc cctggacctt
     1981 caggcattgc accgggaggt cgatcaggtc cggaatgtcc tcgaagagaa agaaagcgtc
     2041 gttcttcaac gccacgctct tttcccacat ctcgtcgccg gagtagagct cccacgactc
     2101 cagacagacg cggtagtcga ggccgccgcg gttcgggctc cccttgaaca ggtccttccc
     2161 gaaagcgtcg gtcgtgcgct cgagccggtg atagggggac atccagcatt tcccgagata
     2221 cgtgtagcac ttgttggcgt ggatcaggat ctcgattccg acgtcgatct gttcggtccg
     2281 ggaggccaac tccttgagag tcagcttcgt gtccgcgacc acctgcgaga cgccgatcga
     2341 acggtagaac cgcacgtcct cgtcgttcag gatgttggcc ccgatgctcg cgtggatcgt
     2401 gagctgcgga aagcgccggt ggacctctgc gatcgcgccg acgtcggtca tgatcgcccc
     2461 gtccacgccc caggacacga agcgctccat cttccggagc atgggagcga tctcgtggga
     2521 ctgcatgttg gtgttgatgg caactctcaa cttgctcggc ccgccgtggg cgatgtcgat
     2581 ggcggcgcgc acgtcgtcgt cgggcatctc gtactggtcg cgccgtcgac tccacccgcg
     2641 gggacccacg taaacggcat ccgcgccgcc cgccaccact gcctcgacca tcggaagatt
     2701 gcctgccggc gcgagaagtt cgaccatgcg cccctccttg acctgacggc ccgtataacc
     2761 gagaacgcga ttccctgttg gctccgccat tcgtgattcc tgcctatgac tccgcgcgcg
     2821 agatcgtcaa caaggacccg atcgattccg ggcggattcc cgcgtagtcc attccactct
     2881 ttccgaagta gaagccgttg cacagcccga tgcgtgaatg tcgcccgagg gtctcccacc
     2941 agcttccgtc gaggtcagcg tctcccgcgg cggcccgatc cagggcggcg cgatatacgg
     3001 ccgcgacctc gcgccggtat tcgggcgtct ccgaaacggt ctcgatccgg aaatgccgat
     3061 ggccttcggc gatcaggcgc ggcaggtgct ccagcatgca cacgtccttg ccgctcagga
     3121 tccccttccc cacggacttc attgcccagt ccccctgctt caggaagagt tccttctggc
     3181 agaggtccgg acacttcacc ccccactttt cctcgtactc gagcaggaag caataatccg
     3241 agatccccag gggcatcttg ccgtgcacca ggagttccat cgccgccccg caagcggtgg
     3301 tgatctcatt gatctcgtcg agactcaact cgtagttggg ggtgaaccgt acggcgccga
     3361 agtcgcgaag gacctcgacc cccgcagcgg tgtagacgtt tgcaaagccc ccgacgtgaa
     3421 ccggcaattg cgggaactcg tcgtgaacga ttttcaagat gcccaggttg tggacttcga
     3481 ccgcttgagc gccggcttcc accgatgctt ccagcatcct gcgcaccttg ggcaggaagt
     3541 cgttccgtgg cgccgcatac gtcgtcacgc aggcccgctt cccctgatcc tggaggatgc
     3601 gaagcccctc ccggagatca ccgagctgct ctaagaagtt tgcctcgtag atgcggcaat
     3661 agatgtcacc caggtagacg gcgtcgtagt cgcggagatc cgactcacgg agatcccgca
     3721 ggttcgcgat cgtcgtattc agctcgaacc ggctcatcgc ttcttgctca cggttcatcg
     3781 ccgttcccta acgtccgacc acggcccacg ccgccggcaa ctcggcaaac ccgaccgcgc
     3841 tcgcgggcgg agccgtcctc gcgtcgattg cttcccggta gcgccgaacg agaggcgcca
     3901 gaagctccac ggggaggctt cgtccctgga gcttcaagac atccacgccc gcttcggcgt
     3961 atgcatggac ctccgcgatc cggctgatct gccggctcgg gaagagacgc gcgtccattc
     4021 gccggccgga agcgtaaagc tcccagggtt gctggcagat cctgtagcag gcgccgacgc
     4081 ccccccgttt catgcttccg gtctgtcgca gcccgcgacg gacgctctcc gtcgcatcgg
     4141 cgtcagcggc ggtttccagc cgcaacacct ccggccggag cgacggcttc agatgcacgt
     4201 aactcggcat ccagcatttt ccgaggagga cgaactcctc gaccatgtg
//
LOCUS       DASWAV010000011         1533 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U2665
            k141_80419, whole genome shotgun sequence.
ACCESSION   DASWAV010000011 DASWAV010000000
VERSION     DASWAV010000011.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960316
            Sequence Read Archive: SRR10260007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 1533)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1533)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:47:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,929
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1533
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_80419"
                     /isolate="SMAG_U2665"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.36444 N 132.31889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1505)
                     /locus_tag="VF958_00245"
     CDS             complement(<1..1505)
                     /locus_tag="VF958_00245"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HEX9147563.1"
                     /translation="MVAEVDKALSGLGFDKVDFGRRLAELSGGQKNRAMLARAILSSP
                     DVLLLDEPTNHLDFQAVEFLEEYLARSRRAYLVVTHDRRFLDRVAGEIIDLENGRIAA
                     YSGGYSAYRRQKAERVLTATRAFEKQQDFIEKEKEYIRRNIAGVNSRQAKGRRTKLAR
                     VERLERPVEDDTSVGFRFDAARIGGRTFLRAKHLDVGYAPGSPIVRGVDFELLRGERM
                     ALLGENGTGKTTLVKTLAGRLAPIAGTVTSGHDVSIGYYDQELSDLDPKRRVIDAVWD
                     EHPDETEEAMRSYLARFAFRGDEVFASISGLSGGEKGRLTLAVVMRQRHNLLVLDEPT
                     NHLDLDSREALEESLEEFPGSIVFVSHDRAFIDRLATRVLDLRAGRARLYDGNYTDTA
                     EARAERRRKPEADAATVVSPQSSVPGPPRQAVRGRRPASEQGKEAARRKRRIESLEEK
                     IATCEKQIEAIETRLWEEGLDLGPVESHRLATEKAARREELEKLVEDWAKLS"
BASE COUNT          221 a          536 c          502 g          274 t
ORIGIN      
        1 gagagcttcg cccaatcctc gacgagcttt tcgagctcct cccgacgcgc ggccttctcc
       61 gtggcgagcc ggtgggactc gacgggtccg agatcgagcc cctcttccca caagcgcgtc
      121 tcgatcgcct cgatctgctt ctcacaggtg gcgatcttct cctcgagcga ctcgattcgc
      181 cgcttgcgac gcgcggcctc ctttccctgc tcggaggcag gacgccgtcc gcgtaccgcc
      241 tgtcttgggg gaccggggac cgaggactgg ggactcacga ctgtcgcggc atccgcctcg
      301 ggctttcttc tccgctccgc ccgcgcttcg gccgtgtccg tgtagttccc gtcgtacaac
      361 ctcgcgcgtc cggctctcag gtcgaggacg cgtgtcgcca ggcggtcgat gaaggctcgg
      421 tcgtgtgaaa cgaacacgat cgagcccggg aactcctcga gcgactcctc gagcgcttcg
      481 cgcgaatcga ggtcgaggtg gttcgtcggc tcgtccagga cgagcaggtt gtggcgttgc
      541 ctcatgacga ccgcgagcgt gagcctcccc ttctcgccgc ccgacagccc ggagatcgac
      601 gcgaacacct cgtccccgcg gaaggcgaaa cgcgcgagat acgagcgcat cgcctcctcc
      661 gtctcatcgg ggtgctcgtc ccacaccgcg tcgatgacgc gccgcttcgg atcgaggtcg
      721 gagagctcct ggtcgtagta gccgatcgag acgtcgtgcc cggacgtcac cgttcctgcg
      781 atgggcgcga gccgcccggc aagcgtcttg acgagcgtcg tcttaccggt cccgttctcg
      841 ccgaggagcg ccatccgctc tccacgcagg agctcgaagt cgacgccccg gacgatcgga
      901 cttccggggg cgtaaccgac gtcgaggtgc ttcgcgcgga ggaaagtgcg cccgccaatc
      961 cgcgcggcgt cgaagcggaa cccgactgac gtgtcgtcct cgaccgggcg ctcgagccgc
     1021 tcgacgcgcg cgagcttcgt gcggcggccc ttcgcctgac gcgagttcac cccggcgatg
     1081 ttgcggcgga tgtactcctt ctccttctcg atgaaatcct gctgcttctc gaaggcccgc
     1141 gtcgccgtca ggacgcgctc cgccttctgc cgccggtacg cgctgtaccc gcccgagtag
     1201 gccgcgatgc ggccgttctc gaggtcgatg atctcgcccg ccacgcggtc gagaaaccgc
     1261 cgatcgtgcg tgacgacgag gtacgcgcgg cgggagcgcg cgaggtattc ctcgaggaac
     1321 tcgaccgcct ggaagtcgag gtggttggtc ggctcgtcga gcagtaacac gtccggcgac
     1381 gagaggatgg cgcgggcgag catggcccgg tttttctggc cgccggagag ctccgcaaga
     1441 cggcggccga agtccacctt gtcgaagccg aggcccgaga gcgccttgtc aacttcggcg
     1501 accatgtcgt atccgccctc gttctcgaat cgg
//
LOCUS       DASWAV010000012         2797 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U2665
            k141_400698, whole genome shotgun sequence.
ACCESSION   DASWAV010000012 DASWAV010000000
VERSION     DASWAV010000012.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960316
            Sequence Read Archive: SRR10260007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2797)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2797)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:47:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,929
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2797
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_400698"
                     /isolate="SMAG_U2665"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.36444 N 132.31889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..697)
                     /locus_tag="VF958_00250"
     CDS             complement(<1..697)
                     /locus_tag="VF958_00250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013494567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid lyase"
                     /protein_id="HEX9147564.1"
                     /translation="MRRLALTGAGLTLEDLVAVSRRRVRVSLAPSARRAMSASRRTVE
                     NVVRRGDRVYGITTGFGRFAEVAIPAEKLEELQQNLVRSHAAGVGTPLPDEAVRAMIA
                     LRANALAAGRSGVRVSTVERLLDLLHADVLPIVPEKGSVGASGDLAPLAHLALGLTGQ
                     GFVRRRGRVVSASRALAAARIEPVTLQAKEGLALINGVQMSVAVGGLALRQALRLARV
                     ADLAGAASLDAARG"
     gene            757..2070
                     /locus_tag="VF958_00255"
     CDS             757..2070
                     /locus_tag="VF958_00255"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014100909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HEX9147565.1"
                     /translation="MKVAAVLGGQWGDEGKGKVVDLLADRFEVVARYHGGHNAGHTVK
                     FADRHFALHLLPAGIVRGATSVIGPGVVVDPDALLSEIASVEEAGIRVEGKLLLSDRA
                     PLILPWHRLLDAAREKAAGAGKLGTTLRGIGPAYESVASRRAVRVAHLSRAATLGDRL
                     AVLHEEMGRLLASYGMEGVPSPEDVLESLLRSEEKLRSHVADTGRWLRDHLARGGSLL
                     AEGAHGAMLDVSAGTFPYVTSSCCTAAGVAEGLHVPPRALDAAVVVLKAYTTRVGEGP
                     FPTELLDATGEFLRSRGNEYGTSTGRPRRTGWFDSVVGRLAVELSGADAIAITKLDVL
                     DDVEEIPVCVGYRLDGRDLPDVPPLCEDYARVEPKLTTLKGWKSKTTAVTRFPDLPPA
                     AKDYVKFLEEQCGAPALLISTGPRREETIWREDLGLFQALPRARR"
     gene            2111..2186
                     /locus_tag="VF958_00260"
     tRNA            2111..2186
                     /locus_tag="VF958_00260"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2144..2146,aa:Lys,seq:ttt)
     gene            complement(2242..2472)
                     /locus_tag="VF958_00265"
     CDS             complement(2242..2472)
                     /locus_tag="VF958_00265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9147566.1"
                     /translation="MLVASTIVMAWNHATVRTAVSDRTSADLAVAINPGLAAQRSAYG
                     STQVVRFRLAINVVISAVTLALSTWALLFRSR"
BASE COUNT          462 a          956 c          948 g          431 t
ORIGIN      
        1 agccgcgggc ggcatcgagc gacgcggcgc ctgccagatc ggcgacccgg gcgagccgaa
       61 gcgcctgccg gagcgcgagg ccgcccaccg cgacggacat ctgcacgccg ttgatgagtg
      121 cgagcccctc ctttgcctgg agggtgaccg gctcgatgcg cgcggcggcg agcgcccgcg
      181 acgcggacac gacgcggccg cgacggcgga cgaagccctg tcccgtcaag ccgagggcga
      241 ggtgggcaag cggcgcgaga tcccccgatg cgccgacgga ccccttttct ggcacgatcg
      301 ggagtacgtc ggcgtggagc agatcgagaa gccgctcgac cgtcgagacc cggacccccg
      361 agcggccggc tgcgagcgcg ttggcgcgca gagcgatcat cgcgcgcacg gcctcgtcgg
      421 gaaggggcgt cccgactccg gccgcgtggc tgcgaacgag gttctgttgg agctcttcga
      481 gcttctccgc cgggatcgcg acttcggcga agcgaccgaa gccggtcgtg atcccgtaga
      541 cgcggtcccc ccgtcgaacc acgttctcga ccgtgcgacg ggaggcggac atcgcgcgcc
      601 gggccgaggg ggcgagcgag acccggacgc ggcggcggga aaccgcgacg aggtcctcga
      661 gcgtcagacc ggcgcccgtc agggcgaggc gtctcatgcg cggagaaagc tatcagctgt
      721 cgtgctatgt tgccccgttt tgcaaaggcc ctctcgatga aagtcgcagc cgttctcggc
      781 ggccagtggg gggacgaggg gaagggaaag gtcgtcgacc tcctcgcgga ccgcttcgag
      841 gtcgtcgcgc gataccacgg cgggcacaac gccgggcaca ccgtgaaatt cgcggaccgg
      901 cacttcgcgc tccaccttct gccggccggc atcgtccgcg gtgcgacttc ggtgatcgga
      961 ccgggagtcg tcgtcgatcc ggatgccctc ctctcggaaa ttgcctcggt cgaggaggcc
     1021 gggattcgag tcgaaggaaa gcttctcctc tccgaccggg cccctctcat cctcccctgg
     1081 caccgtctgc tcgacgccgc gcgcgagaag gccgccggcg cgggaaagct cggcacgacg
     1141 cttcgcggga tcgggccggc ttacgagtcg gtggcctcgc gcagggcggt acgggtcgcg
     1201 cacctttccc gcgcggcgac gcttggcgac cgacttgccg tgcttcacga agagatggga
     1261 aggctcctcg ccagctacgg aatggagggc gtcccctccc ccgaggacgt gctcgagtcg
     1321 ctcctgcgct cggaggagaa gctgcgttcc catgtcgccg acaccgggcg gtggctccgg
     1381 gatcacctcg cccgcggcgg atccctcctc gccgagggag cgcacggcgc gatgctcgac
     1441 gtttcggccg gtacgttccc gtacgtgacg tcgtcgtgct gcaccgcggc cggagtcgcc
     1501 gagggcctcc acgttccgcc gcgcgccctc gacgcggcgg tcgtcgtcct caaggcttac
     1561 acgacccggg tcggcgaagg cccgttcccg acggagctct tggacgcgac cggcgagttc
     1621 ctgcgctcgc ggggcaacga gtacggaaca tccacgggac ggccgcgtcg caccggctgg
     1681 ttcgactcgg tcgtcgggcg cctcgccgtc gagctctcgg gcgccgatgc gatcgcgatc
     1741 acgaagctcg acgtcctgga cgacgtcgag gagatcccgg tctgcgtcgg gtaccggctc
     1801 gacggccggg accttccgga cgtgccgccg ctctgcgagg actacgcgcg cgtggagccg
     1861 aagttgacga cattgaaggg ctggaagtcg aagacgacgg ccgtgacgag attccccgac
     1921 cttccgccgg cggcgaagga ctacgtgaag ttcctcgagg agcagtgcgg agccccggcg
     1981 ctgctcattt cgaccggccc gcgacgcgaa gaaacgatct ggcgcgagga cctcggcctc
     2041 ttccaggctc tcccgcgcgc ccggcgctga tcgatcatcg gcggcagcct ctcgtatact
     2101 tcgcgcccga gggccgttag ctcagtcggt agagcattgg ccttttaagc cagtggtcgt
     2161 gggttcgagt cccacacggc ccaccatctt ttcaagacga tggccgaaag gtccaacgct
     2221 cccacccctg gttggcggga cctatcgaga tcggaacaag agcgcccagg tggagagcgc
     2281 cagcgtgacc gcgctgatga ccacgtttat cgcgagccga aagcgaacaa cctgcgttga
     2341 tccataggcc gatctttgcg cggccagacc cgggttgatg gcgacggcga gatccgcgga
     2401 cgtgcggtcg ctgaccgcgg ttcttacagt ggcgtggttc cacgccatga cgatcgtaga
     2461 cgcgaccagc acccaccaga gaaagccacg aaagggccag agccggtaga gcgcaagcgc
     2521 ggcgagcgca taaatcagat accggaccag ctatctcacg tttcagcctc ctctcgcgac
     2581 taccgcgacc ggcacaattt ccgatcttgc cccctcgccc ttgaggcagg accaagggcg
     2641 ctccgactgt gactcgcctc accagtcgcc tcccgacgac ggctgtccaa atcctcggca
     2701 cccattattt ttgtcatccc gcgtcccccc ggagggacaa aagcggtcgg atcacaagct
     2761 cttctctaaa tggaggttac tgcaaataca cgactta
//
LOCUS       DASWAV010000013         3048 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U2665
            k141_641185, whole genome shotgun sequence.
ACCESSION   DASWAV010000013 DASWAV010000000
VERSION     DASWAV010000013.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960316
            Sequence Read Archive: SRR10260007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 3048)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3048)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:47:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,929
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3048
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_641185"
                     /isolate="SMAG_U2665"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.36444 N 132.31889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..740
                     /locus_tag="VF958_00270"
     CDS             <1..740
                     /locus_tag="VF958_00270"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019398372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="M13-type metalloendopeptidase"
                     /protein_id="HEX9147567.1"
                     /translation="GDLFGNAERVAKFEWDREVNRIGKTVDKEEWGMTPQTVNAYYNA
                     TRNEIVVPAAILQPPFFDPNADPAVNYGGIGAVIGHEVTHGFDDQGRKSDGDGMLRDW
                     WSPEDAAKFEAQAAKLGAQYEAFNFPQLPGLHITGRLTMGENIGDLGGILLGLDAYKR
                     SLNGQPAPVIDGFTGEQRVFLGWGQVWRTLMRDDALRQLLATNPHSPGTVRAFAPLRN
                     VDAWYTAFDVKEGDANYVKPEDRVRIW"
     gene            complement(783..1058)
                     /locus_tag="VF958_00275"
     CDS             complement(783..1058)
                     /locus_tag="VF958_00275"
                     /inference="COORDINATES: protein motif:HMM:NF016876.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HEX9147568.1"
                     /translation="MAYRPLYLPGAEKDIRALPSRVAERVRNAVGRLAENPRLGKRLG
                     GELVPFWSYLVGDYRLIYEIRDDELIVLVVMLGYRRAIYERARRKRP"
     gene            1482..1913
                     /locus_tag="VF958_00280"
     CDS             1482..1913
                     /locus_tag="VF958_00280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9147569.1"
                     /translation="MKRSTRIGLAVILTSVFLPLIALMFTESIHESLVIRFGSISQRA
                     SFEQFPIGRDPGKELGVVLLDVGHATLLLDKEVPKDEANRITREFSRLYGTDPQTKGE
                     VYVFKGWIDEARSALPYRYVVALGLTLLLIGFLIILLGGKS"
     gene            complement(1989..2981)
                     /locus_tag="VF958_00285"
     CDS             complement(1989..2981)
                     /locus_tag="VF958_00285"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEX9147570.1"
                     /translation="MNPSDEELLQALASGREEAIGTIYARYAPTVFGMAAQALDRPTA
                     EEIVQDVFVAVWKNAGSFDARRGPVRPWLLQIAHYRIANELRSRSRRPRIASDPEGER
                     LSGLPDPAPDQTEHAWNEYRHATLRRALEELPAPQRQALGLAFFEDLSHGEIASVLDL
                     PLGTAKSRVRAGLAALRGKLAPLVAMLAFLAMLTGVAFEFRSRRRAFETDERAVAMLT
                     SSDAQALRLTAAAGVRPETHGVYRFRPGATTAVITFSNFSPAPSGRAYQAWALLGGRW
                     TSLGLARPDAVGKARLIAEGAAFAGRPEGLEVTVEPETGSSSPAGPVVIAWRGP"
BASE COUNT          527 a          979 c         1033 g          509 t
ORIGIN      
        1 agggagatct cttcggcaac gcggagcgcg tcgccaagtt cgagtgggat cgcgaggtca
       61 accgcattgg caagacggtc gacaaggaag agtggggcat gaccccgcag accgtcaacg
      121 cgtactacaa cgcaacgaga aacgaaatcg tcgtcccggc cgcgattctg cagccgccgt
      181 tcttcgatcc gaatgcggac ccggcagtga actatggtgg catcggtgcc gtgatcggcc
      241 acgaggtcac gcacggcttc gacgatcagg gacggaagtc cgacggcgac ggcatgctgc
      301 gcgactggtg gtcaccggaa gatgcggcca agttcgaggc gcaggcggcg aagctcggcg
      361 cgcaatacga agcattcaac ttcccgcagc tgcccgggct ccacatcacg ggccgcctca
      421 cgatgggcga aaacatcggc gacctcggcg gcatcctgct gggcctcgat gcttacaagc
      481 ggtcgttgaa cgggcagccg gcacctgtca tcgacggttt caccggcgag cagcgggtgt
      541 tcctcggatg ggggcaggtg tggcggacgc tcatgcgcga cgatgctctt cgccagctcc
      601 tcgcgacgaa cccgcattcg cccggcacgg tccgggcctt cgccccgctc cgcaacgtcg
      661 acgcctggta cacggcgttc gacgtcaagg agggggacgc gaactacgtc aagcccgagg
      721 atcgcgtccg gatctggtag agggcgcgag ccgttcgtat cggaggacgt cgggaagcga
      781 cctcaaggtc gcttccggcg ggcccgttcg tagatcgcac gccggtaccc cagcatcacg
      841 acgagcacga tgagctcgtc gtctcgaatt tcgtaaatga gccggtagtc cccgacgagg
      901 taggaccaga agggaacgag ttccccgccg agtcgcttac cgagccgtgg gttttctgcg
      961 agccgcccga cggcgttgcg gactctctcg gcgaccctgc tcgggagagc ccggatgtct
     1021 ttttcggctc cgggaaggta gagcggccgg taggccatcc ttcctccaca cctgctcgtg
     1081 cgtcagaaaa actcccgcgc ccgcttcgcg gcgagctctc gcgatcttct tcatcgcagt
     1141 ggaatccgaa aggatcgcca gtgtttcgag aaggctttcc cactcctccg ccgacaccag
     1201 gacgccggcc gggatgccgt tgcgcgtgat ttgaacggct tcgccgcgct cttcgacgtc
     1261 gcgcaagagt tcgagaagcg agttcttggc ctgggacgcg gtgaccgtgc tcatgtgatg
     1321 gctattataa tggccgtcga gcccgactct tggaggctcg atcacgcccg ctgcggcggc
     1381 caaaatcctc gagttgcctg ggcgacgctc caacctgcgc cccggcggtt actcggagaa
     1441 tctcgtaaac tgcacccggt ccactccgag cgggagaaaa gatgaagcgc tcaacgcgga
     1501 taggcctcgc ggtgattctg acgagtgttt ttctgccgct gattgcactc atgttcacgg
     1561 aaagcatcca cgaatcgttg gtgatccggt tcggttcgat ttctcagagg gccagcttcg
     1621 agcagttccc gatcggtcgg gaccccggga aggagctcgg agtcgtgctc ctcgacgtcg
     1681 gtcatgccac gctcctcctg gacaaagaag taccgaagga cgaggccaac aggattacgc
     1741 gagagttttc cagactctac ggaacggacc cgcagaccaa aggcgaggtc tacgtcttca
     1801 aaggctggat cgacgaggcc cgaagcgcgc tcccgtatcg gtacgttgtc gccctgggct
     1861 tgactctgct gctcatcggt ttcctgatca tcctgttggg ggggaaatcg tgagcagctc
     1921 cgaggaacgc cgtaacttcg cctgacaccc accccgctcc ttcagcgctt tctcgaaact
     1981 gctcgacctc acggcccccg ccaggcgatg acgacgggac cggcgggcga ggagctgcca
     2041 gtttcgggct cgaccgtgac ctccagacct tcgggccggc ccgcgaacgc ggcgccttcc
     2101 gcgatgaggc gcgccttgcc gaccgcgtcg gggcgggcga ggccgagcga ggtccagcgg
     2161 ccgccaagaa gcgcccaggc ctggtaggcg cggcccgacg gcgccggtga gaagttcgag
     2221 aacgtgatga cggccgtcgt cgcgccgggc cggaagcggt agacgccgtg cgtctccggc
     2281 cggaccccgg ccgccgcggt gagccgaagg gcctgcgcgt cgctcgaagt gagcatcgcg
     2341 accgcgcgct cgtcggtttc gaacgcgcga cggcggcttc ggaactcgaa ggcgacgccg
     2401 gtgagcatgg ccaggaaagc gagcatcgcc accagcggcg cgagcttgcc gcgcagcgcg
     2461 gccaggcccg ctcgcacccg cgactttgcc gtgccgagcg gcagatcgag gaccgaggcg
     2521 atctcgccgt gcgaaaggtc ctcgaagaaa gcgaggccca gggcctggcg ctgtggagca
     2581 ggaagctcct cgagcgcgcg ccgcagggtg gcgtgccggt actcgttcca ggcgtgctcg
     2641 gtctggtcgg gcgcgggatc gggcaggccg gagaggcgct cgccctcggg gtccgatgcg
     2701 atcctgggcc gccggctgcg gctacgcagt tcgttggcga tccggtagtg cgcgatctgc
     2761 aggagccacg gccggacggg ccctcgcctg gcatcgaagg agccggcgtt cttccaaacg
     2821 gcgacgaaga cgtcctgcac gatttcttcg gccgtgggac ggtcgagggc ctgggccgcc
     2881 ataccgaaga ccgtcggtgc atagcgcgcg tagatcgttc cgatggcctc ctcacgcccg
     2941 gaggcgagcg cctggagcag ttcctcgtcg gatgggttca ctggcgcgct gacgatatgc
     3001 atgctaacgc tcggcgtgaa gctcctgccg ctcggcgtcg gtgagctg
//
LOCUS       DASWAV010000014         2796 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U2665
            k141_80534, whole genome shotgun sequence.
ACCESSION   DASWAV010000014 DASWAV010000000
VERSION     DASWAV010000014.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960316
            Sequence Read Archive: SRR10260007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2796)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2796)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:47:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,929
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2796
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_80534"
                     /isolate="SMAG_U2665"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.36444 N 132.31889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..601
                     /locus_tag="VF958_00290"
     CDS             <1..601
                     /locus_tag="VF958_00290"
                     /inference="COORDINATES: protein motif:HMM:NF024644.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HEX9147571.1"
                     /translation="VDPACTNACMIGALKKKEHGIVTYDPNLCVGCRYCQTACPFNIP
                     KFEWKSAAPKIVKCELCRHRAVGASLSSVGGFTRYPKGNGPACCEVCPRDAVVYGTRN
                     ELLEEARRRMKEHPDRYVAKIYGESDAGGTQVIYLSHVPFDKIGLPTLSDRSVPDLQR
                     TIQHGIYQGFVAPVALYGVLGAVLWRNRRKAEKEKGGES"
     gene            598..1800
                     /gene="hybB"
                     /locus_tag="VF958_00295"
     CDS             598..1800
                     /gene="hybB"
                     /locus_tag="VF958_00295"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014780161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe-hydrogenase cytochrome b subunit"
                     /protein_id="HEX9147572.1"
                     /translation="MSARSEALGGPVVTRPFRVLLLLFALAAALILWRLFAGLGATTA
                     MSDGYPWGIWIAFDVVTGTALACGGYAMAILVYVFNKGRYHPLVRSAILTSALGYTIA
                     GVSVVLDLGRPWLVWKIPLFFWSWNLNSALLEVALCIMAYVVVLWIEVSPAFLERWKN
                     GPEGRLQRFSAKAHPFLERYLIWIIALGMLLPTMHQSSLGTLIMLAGPKLHPLWQTPI
                     LPLLFLVSCIAMGYAAVVFESTLSSFFFRRSRDTEMLAGLSGAMLPVLGAFVVLRFGD
                     LVVRGQLRLAFVANGYAAAFWLEIALAVAAFLLLAGRNARRAPGNLFRAAMLMMLAGG
                     VYRFNTYLVAYRPGDDVHYFPSVAEFLITVGLVAFEVLAYVVIVKMFPILGGEAPAEI
                     RVSSRRER"
     gene            1804..>2796
                     /locus_tag="VF958_00300"
     CDS             1804..>2796
                     /locus_tag="VF958_00300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011419537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel-dependent hydrogenase large subunit"
                     /protein_id="HEX9147573.1"
                     /translation="MATRITVDPVTRIEGHLRIDVEVNGGKVQKAWSSGQMFRGIETI
                     LLGRDPRDAWLFTQRFCGVCTTVHAIASVRAVENALGLEVPLNAQYIRNLIIAAHAMH
                     DHIVHFYQLSALDWVDVVSALKADPAKTAALAESLSPWPHNNRLTLAGVQEKLKSFVD
                     AGQLGIFANGYWGHSAMKLSPEVNLLAVAHYLQALEYQRKVNQVVAILGSKTPNIQNL
                     AVGGVANAINLDNEATLNMNKLYMVKDILDEMGAFIEQVYFVDVCAVAAMYPDWLGYG
                     AGVTNYLAVPDFPLDTKGTRFDMPGGTIFNGDLANVREIKSFNDPYFKENVTESI"
BASE COUNT          464 a          943 c          869 g          520 t
ORIGIN      
        1 cgtggatccg gcctgcacga acgcctgcat gatcggagcg ctcaagaaga aggagcacgg
       61 gatcgtcacc tacgacccga atctgtgcgt cggctgccgc tactgccaga cggcgtgccc
      121 cttcaacatt ccgaagttcg agtggaaatc cgcggctccg aagatcgtca agtgcgagct
      181 gtgccggcac cgcgccgtgg gcgcgagcct ctccagtgtc ggcggtttca cccgctatcc
      241 gaagggaaac ggacctgcct gctgcgaagt ctgcccgaga gacgcggtcg tctacggaac
      301 ccgtaacgag ctcctcgagg aagcgcggcg tcgcatgaag gagcacccgg accgctacgt
      361 cgcgaagatc tacggcgagt ccgacgccgg cggcacgcag gtgatctacc tctcgcacgt
      421 gcccttcgac aagatcggcc ttccgactct ctccgatcgc tcggtgcccg acctacagcg
      481 cacgatccag cacgggatct atcaagggtt cgtcgcgccg gtcgcgctct acggggtgct
      541 gggcgccgtg ctctggcgca atcgccgcaa agccgagaag gaaaaagggg gcgaatcatg
      601 agcgcccgct cggaagccct gggcggaccg gtcgtgaccc gcccgttccg cgtcctgctc
      661 ctcctcttcg cgctcgcggc cgcgctgatc ctctggcgtc tcttcgccgg cctcggcgcg
      721 acgacggcga tgtccgacgg atacccctgg ggaatctgga tcgccttcga cgtcgtcacc
      781 ggcacggcgc tcgcgtgcgg cggatacgcg atggccatcc tcgtatacgt tttcaacaag
      841 ggtcgctatc acccgctggt gcgttccgcg atcctgacga gcgccctcgg ctacacgatt
      901 gcgggggtct cggtcgtcct ggatctcggc cgcccgtggc tggtctggaa gatcccgctc
      961 ttcttctggt cctggaacct gaactccgcg ttgctcgagg tcgcgctctg catcatggcg
     1021 tacgtcgtgg tcctctggat cgaggtatca cccgcgttcc tcgagcgctg gaagaacggg
     1081 cccgaggggc ggctccagcg attctcggcg aaagcgcatc cgttcctgga gcgttacctg
     1141 atctggatca tcgcgctggg gatgctgctg ccgaccatgc atcagtcctc actcgggacg
     1201 ctgatcatgc tcgctggtcc caagctccac ccgctctggc agactccgat cctgcccctc
     1261 ctcttcctcg tttcctgcat cgccatgggc tacgcggcgg tcgtgttcga gtcgaccctg
     1321 tcgagcttct tcttccgccg gagtcgggac accgagatgc tcgcggggct ctccggtgcg
     1381 atgctgcccg ttctgggggc gttcgtcgtc ctcagattcg gtgacctcgt ggtgaggggc
     1441 cagcttcggc tggcgtttgt cgccaacggg tacgcggcgg cgttctggct ggagatcgca
     1501 ctcgccgtgg cggccttcct cctgctcgcc ggacggaacg cgcggcgcgc acccggcaat
     1561 ctcttccgcg ccgcgatgct catgatgctc gccggaggcg tctaccgctt caacacgtac
     1621 ctcgtggcgt atcgccccgg ggacgacgtt cactactttc cgagcgtcgc cgagttcctg
     1681 atcaccgtgg gcctcgtggc cttcgaggtc ctcgcctacg tcgtcatcgt gaagatgttc
     1741 cccattctgg gcggcgaagc acccgcggag atccgggtga gctcgcgcag ggagaggtaa
     1801 agcatggcca cacgcatcac cgtcgatcca gtcactcgca tcgagggtca cctccgcatc
     1861 gacgtcgagg tgaacggcgg caaggtccag aaggcctggt cctcgggaca gatgttccgc
     1921 gggatcgaga cgatcctgct cgggcgagat ccgcgggacg cctggctctt tacgcagcgg
     1981 ttctgcggcg tgtgcacgac ggttcacgcg atcgcgtcgg tccgcgccgt ggagaacgca
     2041 ctcggactcg aggtcccgtt gaacgcgcag tacatccgca acctgatcat cgccgcgcac
     2101 gccatgcacg accacatcgt gcacttctat caactgtcgg cgctcgactg ggtcgacgtc
     2161 gtctcggctc tcaaggcgga tccggcgaag acggccgctc tcgccgagag cctgtctcca
     2221 tggccgcaca acaaccgcct cacgctcgcc ggcgtgcagg agaagctgaa gagcttcgtc
     2281 gacgcgggcc agctcggcat cttcgcgaac ggctactggg gccattccgc gatgaagctt
     2341 tcgccggaag tgaacctgct cgccgtcgcg cactaccttc aggccctgga gtaccagagg
     2401 aaggtcaacc aggtcgttgc gatcctcggc tcgaaaacgc ccaacatcca gaacctcgcc
     2461 gtgggcggcg tggccaacgc catcaacctc gacaacgagg ccacgctcaa catgaacaag
     2521 ctctacatgg tcaaggacat cctggatgag atgggggcct tcattgagca ggtctacttc
     2581 gtcgacgtct gcgcggtcgc tgcgatgtac ccggactggc tcggctacgg tgcgggcgtc
     2641 acgaactacc tcgccgtccc ggacttcccg ctcgacacga aaggcacccg gttcgacatg
     2701 ccgggcggaa cgatattcaa cggagacctc gcgaacgtcc gggagatcaa gagcttcaac
     2761 gacccgtact tcaaggagaa cgtcaccgag tcgatc
//
LOCUS       DASWAV010000015         3291 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U2665
            k141_721196, whole genome shotgun sequence.
ACCESSION   DASWAV010000015 DASWAV010000000
VERSION     DASWAV010000015.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960316
            Sequence Read Archive: SRR10260007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 3291)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3291)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:47:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,929
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3291
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_721196"
                     /isolate="SMAG_U2665"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.36444 N 132.31889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1883
                     /locus_tag="VF958_00305"
     CDS             <1..1883
                     /locus_tag="VF958_00305"
                     /inference="COORDINATES: protein motif:HMM:NF018884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HEX9147574.1"
                     /translation="PRYNAPAVSLKKSTRIWLGLFAVSAAAAIILHRVSPDAGCAPTL
                     LTIAAAVACLVLLGRGAVALFRVIVRRTALRLAFSYFLIGIVPIPLLATLAFVLFYVV
                     AHQFVATRVWRETTEIANTATGLPALQVRDGKVVRSDVSWLEPGQPAPWVSQLDRPRA
                     LIEAKNVWIAVRDERDAASVRLLHLNDPSGAWLKKLADRTGYAVRVETGRAQRGRRGF
                     EMNVGSRRTPSKEATAVTVRPSALPAPTKGREWIGAVYVEPAAAIVNATVSKENTVVF
                     IATTSPQVVFEQLFTQGIPRIARIFRVVTMGLAAALLFVYLVALALAFVLVSTITRNV
                     NRMSRAAEGIARGDFSVRVNTKSRDQIGDLARSFDGMADSIQKLLVDTARKERLEAEI
                     AVARTIQQKLLPPASATLDGARILAHVRSVAEIGGDYYDYLPMPDGRSAIVVGDVSGH
                     GLPTGLLVAMAKAGLATLIETGLTGPPLFTRLNDLIHRSTDSRNYMTLALLVYDAKSR
                     RGELTNAGQLAPYRVSKGKVESLSLPSFPLGASERSEFPTRTWDFVAGDTVVLLTDGL
                     VECLSPAGEPFGFERFEAILARESASPPDRLRDAILGEIDAYTGGAAPDDDRTLVIVT
                     LD"
     gene            1918..>3291
                     /locus_tag="VF958_00310"
     CDS             1918..>3291
                     /locus_tag="VF958_00310"
                     /inference="COORDINATES: protein motif:HMM:NF016289.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family peptidase"
                     /protein_id="HEX9147575.1"
                     /translation="MRRSSLAASLLFAAARFVTAQASPFVDERTERLLVNELSGDRAF
                     ETLRITTQWHKPSSSEGFFAVARYVMQQAKDAGLEDVRWIDQVGHSTPWTCRAAEAWL
                     VEGEGAGTKETKLGSFAEVATSIADYSRPANVTADLVDVGAGDGAADYQGKDVRGKIV
                     LAYGNPAAVTEQAVWKRGAAGILAWSSTRLNALADHPDQVAWQRVPEKDGPNGEKTTF
                     AFVLSARDGKALSDRFRGEAARRIFAEGTGVSATKLRVRVVVDSVVSSEKKTAMVEAR
                     LRGADPTLPEIVLTGHLQEEKFSANDDQSGVANVLEIGRALARLVSEGKIPRPRRGIR
                     FWWADEIYSEYRYFADHPGEEKKILADLNEDMVGARQSLGGRVQHMARTPWVRPSFLS
                     DVQESILDMVVSGNTAYLPAFQAHSIPAGVPFSKPIFSHLGSREPYHARAVPYFSNTD
                     HMVFDDSW"
BASE COUNT          508 a         1176 c         1074 g          533 t
ORIGIN      
        1 ctcctcgcta caatgccccc gcggtgtccc tgaagaaatc gacgcgcatc tggctcggcc
       61 tcttcgccgt ctcggcggcc gccgcgatca ttctccaccg cgtgagcccg gacgccggct
      121 gcgcgccgac gctcctcacg atcgccgccg ccgtggcgtg cctcgtgctg ctggggcggg
      181 gagccgtcgc cctcttccgc gtcatcgttc gccggacggc gctgcggctc gcgttctcgt
      241 acttcctgat cgggatcgtt ccgattcccc tgctggcgac cctcgccttc gtcttgttct
      301 acgtcgtcgc gcaccagttc gtggcgacgc gggtctggcg cgagacgacc gagatcgcga
      361 acaccgcgac cggtctcccc gccctccagg tccgcgacgg aaaagtcgtc cggtccgacg
      421 tctcctggct cgagcccgga cagcccgcgc cctgggtctc gcaactcgac cgaccccgcg
      481 cgctcatcga ggcaaagaac gtctggatcg cggttcgtga cgagcgtgac gccgccagcg
      541 tccggctcct tcacctgaac gacccatccg gagcctggtt gaagaagctc gccgaccgga
      601 ccggctacgc cgtccgagtg gaaaccggcc gcgcgcagcg aggccgccgg ggcttcgaaa
      661 tgaacgtcgg gtcgcgccgg acgccgtcga aggaggcgac ggcggtcacc gtgcgaccgt
      721 cggcgctgcc cgccccgacg aagggccgcg agtggatcgg cgcggtgtac gtcgagcccg
      781 cggcggcgat cgtcaacgca acggtcagca aggagaacac ggtcgtcttc atcgccacga
      841 cctcgccgca ggtcgtcttc gaacagctct tcacccaggg gatccccagg attgcgcgaa
      901 tcttccgcgt cgtcacgatg ggtctcgctg ccgcgctcct cttcgtgtac ctcgtcgccc
      961 tcgcgctcgc cttcgtcctc gtctcgacca tcacgcgcaa cgtcaaccgc atgagccgcg
     1021 ccgcagaggg aatcgcgcgc ggtgactttt ccgtccgggt gaacacgaag tcgcgcgacc
     1081 agatcggcga ccttgcgcgc tcgttcgacg ggatggcgga ttcgatccag aagctcctcg
     1141 tcgacaccgc ccggaaggag cgtctggaag cagagatcgc ggtcgcgcga acgatccagc
     1201 agaagctcct cccgccggct tcggcgacgc tcgacggagc ccggatcctc gcgcacgtcc
     1261 ggtcggtcgc ggaaatcggg ggcgactact acgactacct gccgatgccc gacggccgct
     1321 ccgcgattgt cgtcggggac gtctcggggc acggcctgcc gacgggactc ctcgtcgcta
     1381 tggcgaaggc gggcctcgcg actctgatcg agacgggtct gaccggcccg cccctcttca
     1441 cccgattgaa cgacctcatc caccgttcga cggactcgcg caattacatg acgctcgccc
     1501 tcctcgtcta cgacgcgaag tcccggcgcg gcgagctcac gaacgccgga cagctcgccc
     1561 cttaccgcgt gtcgaaggga aaggtcgaga gcctctcgct cccatccttc cctctgggcg
     1621 cgtcggagcg ctccgagttt ccgacgcgga cctgggattt cgtcgccggg gacacggtgg
     1681 tcttgctcac cgacggcctc gtcgagtgtc tgagccccgc gggcgagccc ttcggcttcg
     1741 agcgcttcga ggcgattctc gcgcgggagt cggcgtcgcc gcccgaccgg ctgcgcgacg
     1801 cgatcctcgg ggagatcgac gcctacacgg gcggcgcggc tccggacgac gaccggactc
     1861 tcgtgatcgt cacgctagac taatcggact ttcatcgcga ccccgaagga ggcccggatg
     1921 agacgctctt cgctcgcggc atcgctcctc tttgccgccg cccggttcgt gaccgcccag
     1981 gcgtcgccgt tcgtggacga gagaaccgaa cgcctgctcg tcaacgagct ctccggggat
     2041 cgcgcgttcg agacgctccg catcacgacc cagtggcaca agccctcctc ctccgaagga
     2101 ttcttcgcgg tcgcccgcta cgtgatgcag caggcgaagg atgcgggcct cgaggacgtc
     2161 cggtggatcg accaggtggg acactcgacg ccgtggacct gccgggccgc cgaagcctgg
     2221 ctcgtcgagg gcgaaggcgc cggcacgaag gagacgaagc tcgggtcctt cgccgaggtc
     2281 gccacgtcga tcgccgatta ctcgcgaccg gcgaacgtga cggcagacct cgtcgacgtc
     2341 ggtgcaggtg acggcgccgc ggactaccag ggcaaggacg tccggggaaa aatcgtgctc
     2401 gcctacggaa acccggcggc cgtaaccgag caggccgtct ggaagcgtgg cgccgccggg
     2461 atcctcgcct ggtcgtccac gcgactgaac gcgctcgccg atcaccccga ccaggtcgcg
     2521 tggcagcgtg tgcccgagaa ggacggtccc aacggcgaga agacgacttt cgccttcgtc
     2581 ctctcggcgc gagacggaaa ggccctctcc gaccggtttc gcggcgaagc ggcacgacgg
     2641 atcttcgcgg agggaacggg cgtctctgcg acaaagctcc gggtgcgcgt ggtcgtcgac
     2701 tccgtcgtgt cgtccgagaa gaagaccgcc atggtcgagg cgcgcctgcg cggcgccgac
     2761 ccgacccttc ccgagatcgt gctgaccggg cacttgcagg aggagaagtt ctcggcgaac
     2821 gacgaccagt ccggcgtggc gaacgtcctc gagatcggtc gcgcgctcgc ccgcctcgtc
     2881 tcggagggga agatcccgcg cccgcgccgc gggatccggt tctggtgggc cgacgagatc
     2941 tactcggagt accgctactt cgcggatcac ccgggcgagg agaagaagat cctcgcggac
     3001 ttgaacgagg acatggtcgg cgcgcggcag tccctgggtg gacgcgtgca gcacatggcg
     3061 cgcaccccct gggtgcggcc gtcgtttctc tcggacgtcc aggaaagcat cctcgacatg
     3121 gtcgtctccg ggaacactgc gtatctgccg gcgttccagg cgcactcgat cccggccggg
     3181 gttcccttct cgaaaccgat cttttcccat ctgggaagcc gcgagccata tcacgcgcgc
     3241 gccgtcccct acttctcgaa caccgaccac atggtcttcg acgactcgtg g
//
LOCUS       DASWAV010000016         2787 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U2665
            k141_480933, whole genome shotgun sequence.
ACCESSION   DASWAV010000016 DASWAV010000000
VERSION     DASWAV010000016.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960316
            Sequence Read Archive: SRR10260007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2787)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2787)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:47:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,929
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2787
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_480933"
                     /isolate="SMAG_U2665"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.36444 N 132.31889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..249
                     /locus_tag="VF958_00315"
     CDS             <1..249
                     /locus_tag="VF958_00315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011419534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HypC/HybG/HupF family hydrogenase formation
                     chaperone"
                     /protein_id="HEX9147576.1"
                     /translation="EWTAVVDFFGVRRQVRLDIVDEPVSPGDYILNHVGFAIRRIPSE
                     DIEQTLAMYRELLAQAPGDDLMAADVRGEIQGAGERDE"
     gene            242..1384
                     /gene="hypD"
                     /locus_tag="VF958_00320"
     CDS             242..1384
                     /gene="hypD"
                     /locus_tag="VF958_00320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012631909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016530 - metallochaperone activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase formation protein HypD"
                     /protein_id="HEX9147577.1"
                     /translation="MNSDRLDVAIRQLKFRDPVLARQLAGALARVVSEIGRSPVSVMH
                     VCGSHEQAIAKYGLRATFPRDLNVIMGPGCPVCITDMPEVDEGVALARQGVMVASYGD
                     MLRVRGTVQSLNDAQAEGASVEVVYSAAQAVELARKTDREVVFFATGFETTAVATAAV
                     LQAGPPKNFSVLSAHKYIPPVMEIVAEMPETSVEGFLAAGHAATITGWGIFESFVERH
                     GIPVVVAGFEPLDILAGLVRLVELVRDREPLVVNMFPRCVTREGNLRAQEQLWSVFRP
                     VGGRWRGIAHVPNGNLRLRDEWARFDARRRFTIDLASLWDYAPPALAADCICGDIMSG
                     IASPSDCKLFGKECVPDAPVGACMVSSEGTCKIWHQYGGHPDLAVL"
     gene            1381..2421
                     /gene="hypE"
                     /locus_tag="VF958_00325"
     CDS             1381..2421
                     /gene="hypE"
                     /locus_tag="VF958_00325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014405416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase expression/formation protein HypE"
                     /protein_id="HEX9147578.1"
                     /translation="MKTGLALLEERVTLRQGAGGRAMRALIEDVFLKDAAELAPGEVG
                     PLAMDDGAALKIGDRWLVITTDSHVVHPVFFPGGDIGRLSVCGTVNDLAMMGATEVLA
                     LTCGAVLEEGFPRTDLERIQASMAQACSEAGARIVTGDTKVMGKGELDGIVLNTSGVA
                     VTDRVVRDSGLEPGDVLIVTGTVGDHGLAILAVRHSLELEGELASDVAPLNGLVREAL
                     RAGGDSVVAMKDPTRGGLSSALHEMAEKSGVGILLDETSVPVVAAVRAAGDLLGIDPL
                     HVANEGKAVLGVRRGAAEAVLAALRAHPLGRDAAIIGTCLSERAGSVILDTGFGRRLL
                     AEPDGELLPRIC"
     gene            2462..>2787
                     /locus_tag="VF958_00330"
     CDS             2462..>2787
                     /locus_tag="VF958_00330"
                     /inference="COORDINATES: protein motif:HMM:NF012596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HEX9147579.1"
                     /translation="MSDAIRLERHGAVGVITIARRERFNSLDVETAQELRRAGLQCAR
                     DAELRAVVLRGDGGVFCSGADLKYIRAGGDAADLSYLQKGRETPKGYGAVFQQILEYI
                     HSTISE"
BASE COUNT          419 a          929 c          996 g          443 t
ORIGIN      
        1 gaatggacgg ccgtcgtcga cttcttcggt gtgcggcggc aggtccgcct ggacatcgtg
       61 gatgagccgg tttccccggg cgactacatc ctgaaccacg tcggatttgc gattcgccgc
      121 attccctccg aagacatcga gcagaccctc gccatgtacc gggagctcct cgctcaggcg
      181 cccggcgacg atctcatggc ggccgacgtg cgcggcgaaa tccagggcgc cggtgagcgg
      241 gatgaatagc gaccggctcg atgtcgcgat ccggcagctg aagttccgcg acccggtgct
      301 ggcgcggcag ctcgccggcg cgctcgcgcg cgtcgtttcc gagatcgggc gctctccggt
      361 ctccgtcatg cacgtgtgcg gcagccacga gcaggcgatc gccaagtatg gcctgcgggc
      421 aaccttcccc cgggatttga acgtcatcat gggtccgggg tgtcccgtct gcatcacgga
      481 catgccggag gtggacgaag gcgtcgcgct cgcgcggcag ggcgtgatgg tcgcgtcgta
      541 cggggacatg ctccgggtcc gcgggacggt ccagtccttg aacgacgcgc aggcggaggg
      601 agccagcgtc gaggtcgtct acagcgcggc tcaggcggtg gagctcgcga gaaagacgga
      661 tcgggaggtc gtcttcttcg cgaccggctt cgagacgacc gccgtcgcga cggcggcggt
      721 gctccaggcc ggaccgccga agaacttttc cgtgctctcc gcgcacaaat acataccgcc
      781 ggtcatggag atcgtcgccg agatgccgga gacgagcgtc gagggttttc tcgcagcggg
      841 tcacgccgcg acgatcacgg gctgggggat cttcgagtcc ttcgtcgagc gtcacggaat
      901 tccggtggtc gtcgcgggct tcgagccgct cgacattctc gcgggcctgg ttcgtctggt
      961 cgagctcgtg cgcgaccgcg agccgctcgt cgtgaacatg tttccccgct gcgtgacccg
     1021 tgagggcaac ctccgcgcgc aggagcagct ctggagcgtc ttccggccgg tcggcggccg
     1081 ctggcgcggg atcgcgcacg tgcccaacgg caacctgcga cttcgagacg agtgggcgcg
     1141 cttcgacgcg cgccgccggt tcacgatcga cctcgcctcc ctctgggact acgcgccgcc
     1201 ggcgctggcg gccgactgca tctgtggcga catcatgtcc ggaatcgcgt ccccgtccga
     1261 ctgcaagctc ttcgggaagg agtgcgtacc ggacgcgccg gtcggcgcgt gcatggtctc
     1321 ctccgaggga acctgcaaga tctggcacca gtacggggga catccggacc tggcggttct
     1381 atgaagacgg ggctcgcact gctcgaggaa cgggtcacgc tccggcaggg agccggcgga
     1441 cgcgccatgc gcgcgttgat cgaggacgtc ttcctgaagg acgccgcgga actcgcgccc
     1501 ggagaggtcg ggcccctcgc catggacgac ggagcggcgc tgaagatcgg cgaccgttgg
     1561 ctggtcatca cgaccgactc ccacgtcgtg catccggtct tctttccggg gggggacatc
     1621 gggcggctct cggtctgcgg caccgtcaac gacctcgcca tgatgggtgc gaccgaggtc
     1681 ctcgccctga cctgcggcgc cgtgctcgag gagggatttc cgcgcaccga tctcgagcgg
     1741 atccaggcgt ccatggcgca ggcctgcagc gaggcgggcg cgcgcatcgt cacgggcgat
     1801 acgaaggtca tgggaaaggg ggagctggac gggatcgtgc tgaacacgag cggcgtcgcc
     1861 gtgacggacc gggtcgtgcg cgattcgggg ctcgagcccg gcgacgtgct gatcgtcacg
     1921 gggacagtcg gcgaccacgg cctcgcgatc ctggctgtgc gtcactccct cgagctcgag
     1981 ggcgaactcg cctccgacgt cgcgccgttg aacgggctcg tccgcgaggc gctccgcgcc
     2041 ggcggcgact cggtcgtcgc catgaaagac ccgacccgcg gaggcctctc ttccgctctc
     2101 cacgagatgg cggagaagag cggagtcggc atactcctcg acgaaacgag cgtcccggtc
     2161 gtcgcggcgg tgagggcggc cggagatctg ctgggaatcg acccgctgca cgtcgccaac
     2221 gagggcaaag cggtcctcgg cgtacgacgc ggcgcagccg aagcggtcct cgcggccctt
     2281 cgcgctcatc ccctcgggcg cgacgcggcg atcatcggca cgtgcctctc cgagcgggcc
     2341 ggatccgtga ttctcgacac cggcttcggg cgtcgcctgc tcgcggagcc ggacggcgag
     2401 ctcctcccgc gcatatgctg aaatcgctcg tcgtcgccgc gctgaccacg ggggtgggtc
     2461 gatgagcgac gccattcgtc tcgaacgcca cggagcggtc ggcgtgatca cgatcgcgcg
     2521 gcgcgagcgg ttcaattcgc tcgacgtcga gaccgcccag gagctgcggc gcgccggcct
     2581 ccagtgtgcg cgcgatgcag agctgcgcgc cgtagtcctc cgcggagacg gaggcgtctt
     2641 ctgcagcggg gccgacctga aatacatccg cgccggcggc gacgcggcgg acctctccta
     2701 cctccagaaa gggcgcgaga ccccgaaggg gtacggcgcg gtgttccagc agatcctgga
     2761 gtacatccac agcacgatct cggagat
//
LOCUS       DASWAV010000017         1590 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U2665
            k141_440934, whole genome shotgun sequence.
ACCESSION   DASWAV010000017 DASWAV010000000
VERSION     DASWAV010000017.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960316
            Sequence Read Archive: SRR10260007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 1590)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1590)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:47:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,929
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1590
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_440934"
                     /isolate="SMAG_U2665"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.36444 N 132.31889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(469..945)
                     /locus_tag="VF958_00335"
     CDS             complement(469..945)
                     /locus_tag="VF958_00335"
                     /inference="COORDINATES: protein motif:HMM:NF013224.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HEX9147580.1"
                     /translation="MAIDDIDLRILDMLQRNSKLSQAKIAGAVGLTTPSVNERIKKME
                     RHGMIKGFVALLDHEKMGLPLTAYIDVSLEHPRFEKAFVDDLEKLLAVQECHYLAGDF
                     AYRLKVKAANPGALADFIQHRLLIIRGVARARTCLSLASKKESTLLPLVEEPAKEK"
     gene            1066..>1590
                     /locus_tag="VF958_00340"
     CDS             1066..>1590
                     /locus_tag="VF958_00340"
                     /inference="COORDINATES: protein motif:HMM:NF012451.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ domain-containing protein"
                     /protein_id="HEX9147581.1"
                     /translation="MKKRDYYEVLGVAKGATEADVKKAYRRLARKLHPDVNPGDKTSQ
                     KRFQEVQEAYDVLKDAEKRRAYDRFGHAASSAGFDAEAAEAAAGFGRGGAPFGAGGGA
                     PFGNASFESTDFSELFGNLFGGASRFRTGPEKGEPGYGTIEVPFRDAILGGTATLSLR
                     REKECSTCSGTGRVG"
BASE COUNT          287 a          500 c          515 g          288 t
ORIGIN      
        1 ggggcgggct ccgctgtgat ttccggcgcg tggcactttg cttgcatcct cccggccaaa
       61 ggcaatgtca gcgcaagcaa acggtcccac cacccacgcc ttctgcaccg accgtgcgga
      121 tgaccgcccg gcccggccaa actctttccg ctttttctag ggttcccccc aaaagaaggc
      181 cgtctggccg atccgtccgg cgaaagccgg gcggaagccc tccatttcgt cgcgctcgct
      241 tgctcgggct ctggctcacg ccaccggcgt tcggctcggc aaacaggcct cgaccgaacg
      301 cctgatccct tgctgcgcga gcgctcccgc ggcgcgaggc tcctccctcg attacctcgg
      361 gagactcctc gcgccgcgaa ccggaagctc cattccgtcg cgctcgcttg ctcgggctct
      421 ggctcacgcc accggcgctc ggctcggcgt gccggcgaac cggaagctct acttttcctt
      481 cgcgggctct tcgaccaacg gaaggagagt cgattccttc ttcgaagcga gcgacaggca
      541 cgtccgtgca cgcgcgactc cgcgaatgat gaggagccgg tgctggatga agtcggcgag
      601 agcgccgggg ttggcggcct tcaccttgag gcgataggcg aaatcaccgg ccaggtagtg
      661 gcactcctgg accgcgagca gcttttcgag atcgtccacg aatgctttct cgaagcgggg
      721 atgctcgaga gacacgtcga tgtaagcagt cagcggaagt cccatctttt cgtgatcgag
      781 aagcgccacg aaacccttga tcatcccgtg gcgttccatc ttcttgatgc gctcgttgac
      841 ggagggagtc gtcagcccga ccgcgccggc gatcttggcc tgcgacagct tgctgttgcg
      901 ctgcagcatg tcgaggattc ggagatcgat gtcgtcgatg gccaccggac cccgcttctt
      961 cagaacctaa tagaattaga tatcagacga gttgcggcta ataatattcg accctagtcg
     1021 ggtgtcaatg gcgtaatttc ggctgcgtat aatcgcgcca aaccgatgaa aaagcgcgat
     1081 tactacgagg ttttgggggt cgcgaaggga gccacggagg ccgacgtgaa aaaggcctac
     1141 cggcgcctcg cccgaaagct gcaccccgac gtcaatcccg gcgacaagac ctcgcagaag
     1201 aggtttcagg aagtccagga ggcgtacgac gtcctgaagg acgccgagaa gaggcgggcc
     1261 tacgaccgct ttggtcacgc ggcgtcctca gcgggatttg acgccgaagc ggcggaagcc
     1321 gcggccggat tcggaagggg cggagcgcca ttcggggcgg gagggggtgc gccgtttggc
     1381 aacgcttcct tcgagtcgac cgacttctcc gagctgttcg gaaacctgtt cggcggggca
     1441 tcgcgcttcc gtacggggcc ggagaagggg gagccgggct acgggacgat cgaagtgccg
     1501 ttccgggacg ccatcctggg cggcaccgcg acactctcac ttcggcgcga gaaggagtgc
     1561 tcgacctgca gcgggaccgg ccgtgtcggg
//
LOCUS       DASWAV010000018         5599 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U2665
            k141_400914, whole genome shotgun sequence.
ACCESSION   DASWAV010000018 DASWAV010000000
VERSION     DASWAV010000018.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960316
            Sequence Read Archive: SRR10260007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 5599)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5599)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:47:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,929
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5599
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_400914"
                     /isolate="SMAG_U2665"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.36444 N 132.31889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            172..1104
                     /locus_tag="VF958_00345"
     CDS             172..1104
                     /locus_tag="VF958_00345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013076950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P1 family peptidase"
                     /protein_id="HEX9147582.1"
                     /translation="MRPRKASFPTGYRIGHATDRRARTGCTVILPPPGTIAAAEIRGG
                     APGTRETGVFTPGNLVNEIHGLVFSGGSAFGLAAGDGVAGWLHERNVGFPVGKERVPI
                     VAGAVLFDLAVGSPRHPDSRMGRAACRAAREGPIETGSVGAGTGATVGKLFGVARAAR
                     GGVGLASIRLPGGEIVAALAAVNAFGDVVDPETGRILAGPTVHGSRPVATAHALFSDE
                     LAQSPLAGAATTLVCVATTVAFEPGSLKRVAIEAHDGIARAVRPAHTAVDGDTVFALA
                     PSGPAPPVLMRLRVGAAAAEVVSRAIVAAVRKRR"
     gene            1101..1961
                     /locus_tag="VF958_00350"
     CDS             1101..1961
                     /locus_tag="VF958_00350"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEX9147583.1"
                     /translation="MTFRDRKERSGILSIAGAALLWSTAGLGIKAIADPPLKVVFFRS
                     AIASVALFLYFRPRIRRFTPGVLTGLACYAACLITFVTATKWTTAANAIFLQYSGVVW
                     VLLLSPIVVKEPFRARDAVSIAVAFAGMALFFFGRFDPHGRAGDAIALLSGLFFAGVV
                     LSLRRERGVGAEAVVTWGSVLTAAVALPFVLRDLSLTPKSAVVYLLLGVFQLAAAYVL
                     FVRGIRHVTATEASLVGMLEPIANPIWVLIFLGEKPAPTSIAGGAIVLGAIAWRTLTG
                     PPPEPVPPAD"
     gene            complement(2120..2692)
                     /locus_tag="VF958_00355"
     CDS             complement(2120..2692)
                     /locus_tag="VF958_00355"
                     /inference="COORDINATES: protein motif:HMM:NF024911.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEX9147584.1"
                     /translation="MKFAFIGTHGVGKTTLCFELAALLKKRDRIVEMVHEVARFCPLP
                     INRETTTDAQSWILHTEIAAELAAQHKAEIVICDRSVLDNYCYLLQTGRAPTLEPLVK
                     WWTATYQLLIKVPIVSPPGYDGLRDTDTAFQRKIDETIESVLAEWEISAVRLDKDRRE
                     HWASESLEAILPLLGPVQQPLFDGESEKTT"
     gene            complement(2720..4297)
                     /locus_tag="VF958_00360"
     CDS             complement(2720..4297)
                     /locus_tag="VF958_00360"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX9147585.1"
                     /translation="MLARATALLAACVLASCARAPRPVAKDTLYRHLDGDPATLDPIT
                     TTEEYGILVEEMIFRPLLALDVRGRPVPGLARSWTVSPDGTAYEFRLDPYAEWEDGSP
                     VTSDDVLFTIERIRDPKVPAFNYRELFSEVAGIETPDASTVRVRFSAPYAERLLAFNL
                     PIVSRAAYARAKSAADLDRHPGGSGPYRLERWNTNQSIALVRRPGVPETTAAYRRVVF
                     RIIPDATVRFRAGSRGELDEFRIGRDQLSTARSSRDFAERNRMLKVPQPIEAYLLWNV
                     RHPFLSEALARRALGHAWPREETARRLYPPDGATLVSGPYLPGAAENDPAIRPPAYDP
                     ALAGRLLDEAGWRTGADGLRSRAGRKASFELLYPTGQAVYAALAEILRSSYRKIGVEL
                     VPRALDWAAYSERSDAGEFDSQLTARVFLPPNPDPYPHFHSSQAPPRGQNYGFYRNPE
                     ADRAMETARRETDPARRLEAYRRVHRLLAADPPADFLWGADQYWGLSRKVEGAAVSQL
                     GLFHFLPGPLAWRPARD"
     gene            4341..5033
                     /locus_tag="VF958_00365"
     CDS             4341..5033
                     /locus_tag="VF958_00365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020146936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease H-like domain-containing protein"
                     /protein_id="HEX9147586.1"
                     /translation="MATVVFDIETVGLPWEGLDDGQRTYLTKWARSDEERVRIPESLS
                     LWPLTGKIVVLAMVNPDSGRGRVWYERSDGRLEETSADGLFTFVGDTETAFLAEFWSA
                     ISKFDRFVSFNGRCFDGPFLMLRSASLGIRVTKNLCGYRYAIKPHTDLLDVLSFFGAS
                     RKWNLDFACKAFGVESPKEHGLDGYAVGHYYRENRLREIALYCRRDVEATAHLFQKLE
                     KTLLPALEDTRL"
     gene            5136..5465
                     /locus_tag="VF958_00370"
     CDS             5136..5465
                     /locus_tag="VF958_00370"
                     /inference="COORDINATES: protein motif:HMM:NF018894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HEX9147587.1"
                     /translation="MGRKLAEQRVSDRVPVGAPATIKWGKEELSGFLEVLNLAGGYVA
                     APRAPELGEYVDINFSLPGDHRRFRVRGSVVFVGESAEARSGFGARFERPPAALLDAI
                     KNMNRGH"
BASE COUNT          856 a         1890 c         1952 g          901 t
ORIGIN      
        1 cgagcaccgg cgtcggaacg aggtcctcta ccgctgcgac ggctgccgcc gcgtgttccg
       61 ccgctatcgg gcgatccgcg gagcacgcta ttgcgtgcgg tgcgaccggg cggggagacc
      121 gagccggctg aagcggctcg cgcgggaaac gggaaacggg aaacgggaac ggtgagaccg
      181 cggaaggctt cttttccgac cgggtatcgg atcgggcacg caaccgaccg ccgcgcccga
      241 accggatgca ccgtcatcct gccgccgccc ggaacgatcg ccgcagcgga gatccggggg
      301 ggcgcgccgg gcacgcgaga aaccggcgtc ttcacgccgg gcaacctcgt caacgagatc
      361 cacggcctcg tcttctcggg cggaagcgcc ttcggtctcg ccgcgggtga cggggtggcc
      421 ggctggcttc acgagcggaa cgtgggattc cccgtcggaa aggagcgggt cccgatcgtc
      481 gcgggcgccg tcctcttcga cctcgccgtc ggaagcccgc gccaccccga cagccggatg
      541 gggcgcgcgg cgtgccgggc cgcacgggaa ggaccgatcg agacggggtc ggtcggggcc
      601 ggcacgggag ctaccgtcgg caagcttttc ggcgtcgcgc gcgccgcgag ggggggcgtc
      661 gggttggcgt cgatccgcct tcccggcggc gagatcgttg ccgcgctcgc ggcggtcaac
      721 gcattcggcg acgtcgttga cccggaaacg gggcgaatcc tcgccggccc gaccgttcac
      781 ggaagccggc cggtcgccac cgcccatgcg ctcttctcgg acgagctggc gcaaagtccc
      841 ctcgcgggcg cagcgacgac gctcgtgtgc gtcgccacga ccgttgcctt cgagccgggc
      901 tcgctcaagc gcgtcgcgat cgaggcccac gacgggatcg cccgcgcagt ccggccagcg
      961 cacacggcgg tggacggcga caccgttttc gccctcgcac cgtcggggcc ggcgccgccg
     1021 gtcctcatga gactgcgcgt gggcgcagcg gctgcggaag tcgtctcgcg cgcgatcgtc
     1081 gccgcggtcc ggaagcggcg ttgacgttcc gcgaccgcaa ggagcggagc ggcattctct
     1141 cgatcgcggg ggcggcgctg ctgtggtcga ccgccggcct cgggatcaag gcgatcgcag
     1201 atccgccgct gaaggtggtc ttcttccgat ccgcgatcgc gtcggtcgcg cttttcctct
     1261 acttccggcc acggatccgg cggttcactc cgggcgtcct gacgggcctc gcgtgttacg
     1321 ccgcgtgcct gatcaccttc gtgacggcga cgaagtggac gactgcggcg aacgcgatct
     1381 tcctccagta cagcggcgtc gtgtgggtgc tcctgctctc ccccatcgtc gtcaaggagc
     1441 ccttccgcgc ccgcgacgcc gtctcgatcg cggttgcgtt cgccggcatg gcgctcttct
     1501 tcttcggccg cttcgacccg cacgggcggg ccggcgacgc aatcgcactc ctctcgggtc
     1561 tctttttcgc cggcgtcgtg ctctcgctgc gccgcgagcg cggcgtcgga gcagaagcgg
     1621 tcgtgacgtg gggaagcgtc ctcacggccg cggtcgccct ccccttcgtc ttgagggacc
     1681 tgtcgctcac gccgaagtcg gcagtcgtct atctgctgct gggggtgttc cagctcgccg
     1741 cggcgtacgt gctcttcgtc cgtgggatcc ggcacgtcac cgcgaccgag gcgtcgctcg
     1801 tcggcatgct cgagccgatc gcgaacccga tctgggtgct gatcttcctc ggggagaagc
     1861 cggccccgac ctcgatcgcc ggcggtgcga tcgtgctcgg cgcgatcgcg tggcgcacgc
     1921 tgaccggacc gccgcccgaa cccgtgcctc cggcggacta aaaaaaaagg agtggggtca
     1981 ggtattcacc gatcgccgaa aagctcgttc gggtacgacg gccttgtcac tttgcgaagc
     2041 aaagtgacaa gtggagacct gaccccgtat gtggcaagtg gagacctgac cctcaaagtg
     2101 gcaagtggag acctgacccc taggttgtct tctcgctctc tccgtcgaag aggggttgct
     2161 ggaccggacc gaggagcggc aggatcgcct ccagggactc cgaggcccaa tgctcccggc
     2221 ggtccttgtc gagcctcacg gcggagatct cccactccgc gaggaccgac tcgatcgtct
     2281 cgtcgatctt ccgctggaac gcggtgtcgg tgtctcgcag cccgtcgtat ccgggggggc
     2341 tcacgattgg caccttgatc aggagctgat aggtcgccgt ccaccacttc acgagcggct
     2401 cgagcgtcgg cgcgcggccg gtctgcagca ggtagcagta gttgtcgagc acgctccggt
     2461 cgcagatgac gatctccgcc ttgtgctgcg cggcgagctc ggccgcgatc tccgtgtgga
     2521 ggatccagga ctgcgcgtcg gtcgtcgtct cgcggttgat cgggagcggg cagaagcgcg
     2581 cgacctcgtg aaccatctcg acgatccggt cgcgtttctt gagcagcgca gcgagctcga
     2641 agcagagcgt cgtcttgccg acgccgtggg ttccgatgaa cgcgaatttc acagtccgaa
     2701 gctatcagct gtcagtcgct caatctctcg cgggtctcca ggcgagcgga cccggcagga
     2761 agtgaaagag tccgagctgc gaaacggccg cgccttcgac cttccgcgac agtccccagt
     2821 actggtccgc gccccagagg aaatcggccg ggggatcggc cgcgagcaac cggtggacgc
     2881 ggcggtacgc ctccagccgg cgcgccggat ccgtttcgcg ccgcgccgtt tccatcgcgc
     2941 ggtccgcctc ggggttccgg tagaagccgt agttctgacc ccgcggcggg gcctggctcg
     3001 aatggaagtg cgggtaggga tccggattcg gcggaagaaa cacccgcgcc gtgagctgcg
     3061 aatcgaactc ccccgcgtcg gatcgttccg agtacgctgc ccagtcgagg gcgcgcggaa
     3121 cgagctcgac gccgattttc cggtaggagc tgcgcaggat ctcggcgagt gcggcataaa
     3181 cggcctgccc ggtgggatag aggagctcga acgatgcctt gcggccggcg cgcgacctca
     3241 gcccatccgc ccccgtccgc catcccgctt cgtcgagaag tcggccggcg agcgcggggt
     3301 cgtacgcggg cggccggatt gccggatcgt tctccgcggc gcccgggagg tacggcccgg
     3361 aaacgagcgt cgcaccgtcg ggagggtaga ggcgccgcgc agtttcctcg cgtggccagg
     3421 cgtgcccgag cgcccggcgc gcgagagcct ccgagaggaa tggatggcgg acgttccaga
     3481 gaaggtacgc ctcgatcggc tgcgggacct tcagcatccg gttccgctcg gcgaagtcgc
     3541 gtgagctccg cgccgtggaa agctgatcgc ggccgatgcg gaactcgtcg agctctccgc
     3601 gggagccggc acggaagcga acggtcgcgt ccgggatgat ccggaagacg acgcgccggt
     3661 aggcggcggt ggtctccggt acgcccgggc ggcggacgag cgcgatcgac tggttcgtgt
     3721 tccaccgctc cagccggtac ggaccgctcc ccccaggatg gcggtcgagg tccgcggccg
     3781 acttcgcgcg cgcgtacgcc gcccgcgaca cgatcggcag gttgaaggcg agaagccgct
     3841 ccgcgtacgg cgccgagaag cgcacgcgca ccgtggaggc gtccggggtc tcgatgccgg
     3901 cgacctcgga aaagagctcg cgatagttga acgccggcac cttcggatcg cgaatccgct
     3961 cgatcgtgaa gagcacgtcg tcggacgtca ccggggagcc gtcctcccac tcggcgtacg
     4021 gatcgagccg gaactcgtac gccgttccgt ccggagagac cgtccaggat ctcgcgagcc
     4081 ccggcacggg ccgtccccga acgtcgaggg cgaggagcgg ccggaagatc atctcttcga
     4141 cgaggattcc gtactcctcg gtcgtcgtga tcggatcgag cgtcgcggga tccccgtcga
     4201 ggtggcggta gagcgtgtcc ttcgcgacgg ggcggggggc acgggcgcag ctcgccagca
     4261 cgcacgccgc gagcagggcg gtggcgcgcg cgagcaatcg cttcattcgc tgagagcaaa
     4321 aagaatagca tccccccatc gtggcgaccg tggtcttcga catcgagaca gtcggcctac
     4381 cctgggaagg actcgacgac gggcagcgga cgtacctgac gaagtgggcc cgcagcgacg
     4441 aggaacgcgt ccgcattccc gagtcgctct ccctctggcc gctcaccggc aagattgtcg
     4501 tcctcgcgat ggtgaacccc gattccggcc gcgggcgcgt ctggtacgag aggagcgacg
     4561 gccgactcga agagacctcc gccgacggac tgttcacgtt cgtcggcgac acggagaccg
     4621 cctttctcgc cgaattctgg agcgcgatct cgaagttcga tcgcttcgtc tcgttcaacg
     4681 gacgctgttt cgatgggccc ttcctgatgc tgcgcagcgc ctcgctcggc atccgcgtga
     4741 cgaagaacct gtgcggctac cgctacgcga tcaagccgca caccgacctt ctcgacgtgc
     4801 tctccttctt cggagcctcg cgcaagtgga acctcgactt cgcctgcaag gcgttcgggg
     4861 tcgagagccc gaaggagcac gggctcgacg ggtacgcggt cggccattac taccgggaga
     4921 accggctgcg cgagatcgcg ctctactgcc gccgcgacgt cgaggcgacg gcgcatcttt
     4981 tccagaagct cgagaaaact ctcctgccgg cgctcgaaga cacgcgcctc tgaggatcga
     5041 tcttcgttct ccccgccgac ggggagaggg tcagggtgag gggcgtttct gctattcttt
     5101 ttcgacttca ccggcaacct gcgcagagag agggcatggg ccgcaaactc gccgaacaga
     5161 gagtctcgga ccgcgttccg gtcggcgcgc cggcgacgat caagtggggc aaggaggagc
     5221 tctccgggtt tctcgaggtc ttgaacctcg ccggaggcta cgtggccgcg ccgcgcgctc
     5281 cggaactcgg cgagtacgtc gacatcaact tctccctccc cggcgaccac cgaaggttcc
     5341 gggtgcgcgg gagcgtcgtg ttcgtcggcg agtcagcgga ggcccgatcc ggcttcggcg
     5401 cgcgcttcga gcgaccgccg gccgcccttc tcgacgcgat caagaacatg aaccggggac
     5461 actagggacc cagccggtca gcgcgcccgc gcctccacca gcctcatccg gtcgaacgcg
     5521 aactccacgg tcgagtccac ctggttgacg tttccggcgg ccgccgaaga gaacgtcacg
     5581 gggttgactc cggtgaagt
//
LOCUS       DASWAV010000019         3429 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U2665
            k141_521091, whole genome shotgun sequence.
ACCESSION   DASWAV010000019 DASWAV010000000
VERSION     DASWAV010000019.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960316
            Sequence Read Archive: SRR10260007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 3429)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3429)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:47:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,929
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3429
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_521091"
                     /isolate="SMAG_U2665"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.36444 N 132.31889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            149..844
                     /locus_tag="VF958_00375"
     CDS             149..844
                     /locus_tag="VF958_00375"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX9147588.1"
                     /translation="MIGKVILVVDDDPGIRESLSKELRSAGYETITASDGREGLASSR
                     SRAPDLILSDLAMPVSDGYELISAIRAFSRVPIIVLSVRSGDPDKVRALDLGADDYVT
                     KPFSVTELLARVRAQLRRAEKAQSPVLEFDDLTIDLERRRVVQGGRDVKLTPTEFALL
                     ELLAANAGTPILLDQIIARVWHGAPATTGDTVRVHMSALRRKIEPDSANPRYIVNEPW
                     VGYRFIAEPLGNS"
     gene            892..1983
                     /locus_tag="VF958_00380"
     CDS             892..1983
                     /locus_tag="VF958_00380"
                     /inference="COORDINATES: protein motif:HMM:NF019268.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
                     /protein_id="HEX9147589.1"
                     /translation="MSRIALLFVSLLPFASVAVAQETTTPAPSPTPTPVVNVHGFVDV
                     YYAWNPNRPPEHDNFIPGTGSTAKHGNELGLNLAALTLSHAPDPVGFTLVLNAGSGTD
                     VIHSGEPANGRDVYRAIYQASVVYEPGRPGGVQIEAGVFPCHVGFEGFFSKDNWNYTR
                     SWLGEFSPYYSTGVKVGFPLGNHLRAQVHVLNGWQIIRDNNDAKTIGTQLLWSSDRVL
                     TAFNTMYGAELPGDESSKRAFGDLWVSFKATPAFTVAASLDAGRQDLPEAPTAKWWGA
                     AGYARFAFTDASAVALRLERFDDPDGGIAGSPQLLDEATLTLEHRPRPNLILKLEGRY
                     DNADDPVFRDKNGLEDSQLLFILGVVAVF"
     gene            2168..2563
                     /locus_tag="VF958_00385"
     CDS             2168..2563
                     /locus_tag="VF958_00385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9147590.1"
                     /translation="MLALFAVSGWTAAALGVYAIELGSSGLLSGLSSAQCTLAKIAFV
                     FAMGAVFGRVATRASLEMALRTGLAWLVFSIAADLVTSLQSARDTDRLLGDPSAPQLL
                     RDLMMLAWVAGPSVFARRVTPDGHSEVFR"
     gene            2658..2747
                     /gene="kdpF"
                     /locus_tag="VF958_00390"
     CDS             2658..2747
                     /gene="kdpF"
                     /locus_tag="VF958_00390"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0031004 - potassium ion-transporting
                     ATPase complex [Evidence IEA];
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="K(+)-transporting ATPase subunit F"
                     /protein_id="HEX9147591.1"
                     /translation="MGWEDVLGLVITVGLLYYLLRALLKAEKL"
     gene            2744..>3429
                     /locus_tag="VF958_00395"
     CDS             2744..>3429
                     /locus_tag="VF958_00395"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: protein motif:HMM:NF015753.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpA"
                     /protein_id="HEX9147592.1"
                     /translation="MTPLKVTEFLIYAALITAATPILGGFMKNVFSGEKTFLDPALRP
                     VERLVYRLCRVDASRDQTWIEWTTSMLLVNAASLVLLYAFQRLQKSLPLNPNGLGPVS
                     PDSSWNTAVSFTTNTNWQGYSGESTMSHFTQMVGLAYHNFLSAATGIAIAVVIIRGIL
                     LEKARGIGNFWFDCTRAMLWVLLPLSFAAALFFVSRGVVQNFRADTTVTTVEGETQKI
                     PGGPFSSQEA"
BASE COUNT          544 a         1141 c         1066 g          678 t
ORIGIN      
        1 tcccgggccg gggtctcggg ttggagattg cacgaagtct cgcggcggcg agcggcggct
       61 cgatcggcct gtcgaatcga ccggggggcg gcgccgtcgc ccggctcgat cttccgacgg
      121 ccggggccgc cgtcgagaag atcgaaaggt gatcggaaag gtcattctcg tcgtcgacga
      181 cgatccggga atccgcgagt ctctgtcgaa ggagctccgg tcggccgggt acgagacgat
      241 tactgcgtcg gacggacgag agggcctcgc cagctcacga tcccgcgctc ccgatctgat
      301 cctgtcggac ctcgcgatgc ccgtttcgga cggctacgag ctgatctccg ccatccgcgc
      361 attctcgcgg gtgccgatta tcgtgctgtc ggttcgcagc ggagatccgg acaaggtccg
      421 cgcgctcgat ctcggcgccg acgactacgt gacgaaaccg ttttccgtca cggagcttct
      481 cgcgcgagtt cgcgctcagc ttcgccgggc cgagaaagcg cagtctcccg tgctcgaatt
      541 cgacgacctc acgatcgatc tcgaacgccg ccgggtcgtg cagggcgggc gcgacgtgaa
      601 gctcacgccg accgagttcg cgctcctcga gttgctcgcc gcgaacgccg ggacgccgat
      661 tctgctggac cagatcatcg cgcgggtctg gcacggagct ccggcgacga cgggcgacac
      721 cgtccgggtg cacatgagcg ctcttcggcg aaagatcgag cccgactccg ccaacccgcg
      781 gtacatcgtg aacgagccgt gggtcgggta ccggttcatt gcggagccgc tcggcaactc
      841 ttagcggagt ctttcggctt tcctcggccg ggtcggggtg atcctcgact catgagccga
      901 atcgctcttc tgttcgtttc cttgttgccg ttcgcgtccg tcgccgtcgc gcaggagacg
      961 acgacccccg cgccatctcc gacgccgacg ccggtcgtca acgttcatgg attcgtcgac
     1021 gtgtactacg cctggaatcc gaaccgccct cccgaacacg acaacttcat tcccggaacg
     1081 ggatccaccg cgaaacatgg aaacgagctc ggtttgaatc tggccgcgct gacgctgtcg
     1141 cacgcgcccg atcccgtcgg cttcacgctg gtcttgaacg ccggcagcgg gaccgacgtg
     1201 attcactccg gcgagccggc gaacgggcgc gacgtctacc gggcgatcta ccaggcctcc
     1261 gtcgtgtacg agccaggccg tccgggaggt gtccagatcg aggcgggcgt ctttccctgc
     1321 cacgtggggt tcgaagggtt cttttcgaaa gacaactgga actacacgcg gtcgtggctg
     1381 ggcgaattct ccccgtatta cagcaccggc gtgaaagtgg gattcccgct cgggaaccat
     1441 ctccgcgcgc aggtgcacgt cttgaacggg tggcagatca ttcgggacaa caacgacgcc
     1501 aagaccatcg ggacgcagct cctctggagc tcggaccgtg tcttgacggc cttcaacacg
     1561 atgtacggcg cggagcttcc gggcgacgag tcctccaaac gcgccttcgg cgatctctgg
     1621 gtgagcttca aggccacgcc ggcgttcacc gtcgccgcct ccttagacgc cggcaggcag
     1681 gatcttcccg aggcacccac cgccaagtgg tggggagcgg caggctacgc tcggttcgcc
     1741 ttcacggacg cgtccgccgt cgcgctacgc ctggagcgct tcgatgaccc ggacggcggc
     1801 atcgccggat cgcctcagct cctggacgag gcgaccttga ccctcgagca tcgcccccgt
     1861 ccgaacctga tcctgaagct cgagggtcgg tatgacaacg cggacgatcc ggtctttcga
     1921 gacaagaacg gcctcgagga ttcgcagttg ctcttcattc tcggcgtcgt cgccgtcttc
     1981 tgaacaatcc gagtttcgat tccttatgaa gtctttcgtt tctctttcgg cactctttcg
     2041 agccctcccg ccgggctgat gcagcatgga gagcgtaatg tcggcaccct tcgccaccga
     2101 acgtgaacga gacagcggaa gcgtcgtccg tttccgccgg gagccgcggc gcccggagtc
     2161 ctcacgcatg ctcgcgctgt tcgcggtttc cggttggacc gcggcagccc tgggggtgta
     2221 tgcgatcgag cttggctcga gcgggctgct gtccggattg tcctcggctc agtgcactct
     2281 ggcgaagatc gccttcgtgt tcgcaatggg cgcagtcttc gggcgtgttg caacgcgcgc
     2341 ttcgctcgag atggcgctca ggaccggtct cgcctggctc gtcttttcca tcgcagccga
     2401 tctcgtaacg agtctccagt cggcacgaga cacagaccgc ctccttggcg atccctctgc
     2461 tccccagctt ctccgagacc tgatgatgct cgcgtgggtt gcggggccgt cggtcttcgc
     2521 ccggcgcgtc acacccgacg gccactccga ggtcttccga tgaaggacgt ggtctttctc
     2581 ctcctgaccg caatcctctt cgccgcttcg atctggtaca cgcggcgctg cgaccggatc
     2641 taggagcgac cggaatcgtg ggctgggaag acgtactcgg tctggtcatc acggtggggt
     2701 tgctctacta cctgctgcgc gcgttgctca aggcggagaa gctgtgaccc cactcaaagt
     2761 cacggagttc ctcatctacg ccgcgctcat cacggccgcg acgccgatcc tcggcggctt
     2821 catgaagaac gtgttctcgg gcgagaagac gttcctcgac cccgctcttc gtccggtcga
     2881 acgtctcgtc taccggctct gccgcgtcga tgcgtcgcgc gatcagacct ggatcgagtg
     2941 gacgacctcg atgctgctcg tcaacgctgc ttcgctggtc cttctctatg cgtttcagcg
     3001 tctgcagaag tcccttccgt tgaacccgaa cggactcggg cccgtgtcgc cagattcctc
     3061 ctggaacacg gcggtctcct tcacgaccaa cacgaactgg caggggtact ccggtgaatc
     3121 gacgatgtct cacttcaccc agatggtcgg cctcgcgtat cacaacttcc tctcggcagc
     3181 gaccggcatc gccatcgcgg tcgtgatcat ccgcggaatc ttgctcgaga aggcgcgcgg
     3241 gattggcaac ttctggttcg actgcacgcg ggcgatgctg tgggtccttc tgccactctc
     3301 cttcgccgcc gcgctgttct tcgtgtcgcg cggcgtcgtg cagaacttcc gcgcggacac
     3361 aaccgtcaca acggtcgaag gagagaccca gaagatcccg ggcggaccgt tttcttcgca
     3421 ggaggcgat
//
LOCUS       DASWAV010000020         4474 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U2665
            k141_681274, whole genome shotgun sequence.
ACCESSION   DASWAV010000020 DASWAV010000000
VERSION     DASWAV010000020.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960316
            Sequence Read Archive: SRR10260007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 4474)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4474)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:47:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,929
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4474
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_681274"
                     /isolate="SMAG_U2665"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.36444 N 132.31889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..750
                     /locus_tag="VF958_00400"
     CDS             <1..750
                     /locus_tag="VF958_00400"
                     /inference="COORDINATES: protein motif:HMM:NF014017.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HEX9147593.1"
                     /translation="RSSRASKSRSPRFSESGAMRVFTGTSGFSYKEWKGSFYPEDLPD
                     SGMLRYYASRLPAVEINNTFYRMPRAALLESWAEQVPDGFTFALKASQQITHRKRLKD
                     AGEALAYFFGVATTLGDRLGPALFQLPPNLKKDLPRLKDFLALLPPASRAAFEFRHES
                     WFDAEVFDALRSARAALCWAEDENLSTPPEPTADWGYLRLRRPDYGEADLAARADRIR
                     NQPWSDAFIFFKHEEEGTGPRLAEAMRALFE"
     gene            787..1446
                     /locus_tag="VF958_00405"
     CDS             787..1446
                     /locus_tag="VF958_00405"
                     /inference="COORDINATES: protein motif:HMM:NF015438.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MOSC domain-containing protein"
                     /protein_id="HEX9147594.1"
                     /translation="MRIDSVAVGKPRVVQWLGRDVRTSIFKSPVTGRIPVRRHNLEGD
                     RQSDLRVHGGEFKAVYAYAAESYDWWKTALGHDLEPAAFGENLTIRDFDEESIAIGDV
                     FRVGDAELEAAAPRLPCYKLGLRFGDAHMVQRFATAGRWGIYFRVVREGLLEAGDALE
                     QIHSDPTGLAVYEVARVFFTDRDDRSVIERLAAHERLDPSWRSHFAEKLRDPDLGRVQ
                     R"
     gene            1443..1727
                     /locus_tag="VF958_00410"
     CDS             1443..1727
                     /locus_tag="VF958_00410"
                     /inference="COORDINATES: protein motif:HMM:NF014229.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UBP-type zinc finger domain-containing protein"
                     /protein_id="HEX9147595.1"
                     /translation="MRRSVADCPHGAPDLPAPRRRSFFRLRAASEESACAECGERDDL
                     RVCQTCGYVACCESHGGHDTDHFEKTGHWFIRPRIGEAWLWCYGCRAYLE"
     gene            1803..2675
                     /locus_tag="VF958_00415"
     CDS             1803..2675
                     /locus_tag="VF958_00415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011985253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045027 - DNA end binding [Evidence IEA];
                     GO_process: GO:0006303 - double-strand break repair via
                     nonhomologous end joining [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ku protein"
                     /protein_id="HEX9147596.1"
                     /translation="MSARALGNASISFGLVSIPVKLYTASNSSASISFNLLHKKCGSR
                     LKQQYICSKEETIVPRDEMVKGYEFNKDQYVQFTDEELKAFEEQATQTIEVVEFVPVD
                     KVDPVYYDKPYYLGPDKGGAKPYRLLAEVMKETGRVALAKYAARGKQYLVMLRPSGQG
                     LVMQQLLYADEVRPFSEVPLDTADVKEGELKLARMLVEESSSDEFRPQAYEDEVRKRV
                     QEALDRKVAGGEFSVTAPQKKGGEIVDLMEALRASLGKPRKAAAPKAVSAAERKPPRS
                     APRTPEKRAASSRK"
     gene            2697..3551
                     /locus_tag="VF958_00420"
     CDS             2697..3551
                     /locus_tag="VF958_00420"
                     /inference="COORDINATES: protein motif:HMM:NF024816.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX9147597.1"
                     /translation="MKPSPGTDAGGGYGAREVSQMLGLTVGQVRAWVRSGLVDPERGP
                     RGELRFSPQDLLFLRAARGLAGARIGPRRIERALSKLREQVSAAESIGDLEIRAEGRT
                     IVVTDGSARWQPESGQMLMEFEEKIGTCVPFPPPSLRPSSGVSGRESASEWYQRGCEL
                     EETDPDAAREAYANALSFDASHADAHVNLGRLLHEAGEAAAAEEHYKSALASRPDDVT
                     AAFNLGVALEDLGRDIEAIDAYEKAVSLDPQHSDAYFNLAGACERVGRPAAAIRHLKA
                     YRALVRSQ"
     gene            3548..>4474
                     /locus_tag="VF958_00425"
     CDS             3548..>4474
                     /locus_tag="VF958_00425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008480761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HEX9147598.1"
                     /translation="MTVDRGATPADPAEKGKSNRRRAGRRPGGFSLLTADDLHLFNEG
                     THTRLWERLGAHPATVAGRAGTNFATFAPEAQAVSVVGDFNGWNPDTHPLESRGRSGL
                     WEGFVPEVGTGALYKFHVRSRHAGYRVDKTDPFAFSRETPPKTGSIVWDLSYEWTDSG
                     WMEDRAKRSARDRPMSIYEVHLGSWMRPDGRLPGYREIAPVLCEYAKSMGFTHVELLP
                     VMEHPFYGSWGYQTTGFFAPTSRYGGPQDLMFLIDQLHENGIGIIFDWVPSHFPSDEH
                     GLVFFDGSYVYEHADPRQAVQPDWGSLVFNYGR"
BASE COUNT          744 a         1518 c         1521 g          691 t
ORIGIN      
        1 cgctcctccc gggcctcgaa gtcccgctcg cctcgatttt ctgaaagcgg cgccatgcgc
       61 gtcttcaccg gcacgagcgg gttctcgtac aaggagtgga aggggagctt ctaccccgag
      121 gacctgcccg actcggggat gctccgctac tacgcgtcgc gtctgccggc ggtcgagatc
      181 aacaacacct tctaccggat gccgcgcgct gcgctcctcg agagctgggc cgagcaggtc
      241 cccgacgggt tcacgttcgc gctcaaggcc tcgcagcaga tcacgcaccg aaagcggctc
      301 aaggacgccg gtgaagccct cgcgtacttc ttcggcgtcg caacgacgct cggcgaccgt
      361 ctcggacccg cgctctttca gctgccgccg aacctgaaga aggacctgcc gcggctgaag
      421 gacttcctcg cgcttctccc gccggcctcc cgcgccgcat tcgagttccg gcacgagtcc
      481 tggttcgacg ccgaggtctt cgatgcgctg cggtcggccc gcgccgcgct ttgctgggcc
      541 gaggacgaga acctctcgac cccgccggag ccgacggccg actgggggta tctgcgactg
      601 cgccgccccg actacgggga agcggacctc gccgctcgtg cggaccggat ccgcaaccag
      661 ccgtggagcg acgctttcat cttcttcaag cacgaggagg agggaacggg gccacggctc
      721 gccgaggcaa tgcgcgcgct cttcgagtga ccgaatccct tcgcctcttc acggcgtaag
      781 acgggcatga ggatcgactc ggtggccgtc ggaaaacccc gcgtcgtgca gtggctcggc
      841 cgggacgtcc gcacgtcaat cttcaagtcg cccgtcactg gacggattcc ggtgcgccgg
      901 cacaacctcg aaggggaccg gcagtccgac ctccgcgtcc acggcggaga gttcaaggcc
      961 gtctacgcgt acgccgcgga aagctacgac tggtggaaga cggcgctcgg gcacgacctc
     1021 gagccggcgg ccttcgggga gaacctgacg atccgggact tcgacgagga atcgatcgcg
     1081 atcggcgatg tcttccgcgt cggggacgcg gagctcgaag ccgccgcgcc gcgcctcccg
     1141 tgctacaagc tcgggctccg cttcggagat gcccacatgg tgcagaggtt cgcgacggcc
     1201 gggcgctggg gaatctactt ccgagtcgtc cgcgaggggc tcctcgaagc cggcgacgcg
     1261 ctcgagcaga tccactccga cccgaccggc cttgccgtgt acgaggtcgc gcgcgtcttc
     1321 ttcaccgacc gcgacgaccg cagcgtcatc gagcgactcg ccgcgcacga gcggctcgac
     1381 ccttcgtggc gcagtcattt cgcggagaag ctccgggatc ccgacctcgg ccgggtgcaa
     1441 agatgaggcg ctcggtcgcg gactgccctc acggtgcgcc ggatctgccc gcgccccgca
     1501 ggagatcctt cttccggctc cgggcggcgt ccgaagagag tgcctgtgcg gagtgtggcg
     1561 agagggatga tctccgggtc tgccagacgt gcggatacgt cgcatgctgc gagtcgcacg
     1621 gcgggcacga cacggaccat ttcgagaaga ccgggcactg gttcattcgg ccgcgcatcg
     1681 gcgaggcctg gctctggtgc tatgggtgcc gggcctacct ggagtgacag cgggtccacc
     1741 cctcaccccc gtcccctctc ccggagggag aggggggagc ggatgctaaa atctcggcca
     1801 acatgagcgc cagagcactc ggcaacgcca gcatctcgtt cgggctcgtc tcgatcccgg
     1861 tcaagctcta caccgcctcg aacagttccg cctcgatctc gttcaatctg ctccacaaga
     1921 agtgcggctc ccgcctgaag cagcagtaca tctgctcgaa ggaggagacg atcgtcccga
     1981 gggacgagat ggtgaagggg tacgagttca acaaggacca gtacgtccag ttcaccgacg
     2041 aggagctgaa ggcgttcgag gaacaggcga cccagacgat cgaggtcgtg gagttcgtgc
     2101 cggtcgacaa ggtcgatccg gtctactacg acaagccgta ttacctcggc ccggacaagg
     2161 gaggtgcgaa gccctaccgt ctgctcgccg aagtcatgaa agaaaccggc cgcgtcgcgc
     2221 tcgcgaagta cgccgcgcgg ggcaagcagt acctcgtcat gctgcggccg tcgggccagg
     2281 gcctcgtcat gcagcagctc ctgtacgccg acgaagtgcg gccgttctcc gaagtgccgc
     2341 tcgacaccgc cgacgtcaag gaaggggagc tcaagctcgc gcgcatgctc gtcgaggaga
     2401 gctcctccga cgaattccgc ccgcaggcct acgaggacga ggttcgcaag cgcgtccagg
     2461 aagcgctcga ccgcaaggtc gctggcggag agttctccgt gaccgccccg cagaagaagg
     2521 ggggcgagat cgtcgacctc atggaggcgc tgcgcgccag cctcgggaaa ccgcgcaagg
     2581 ctgccgcccc gaaggcggta tcggcggcgg agcggaaacc tccccgcagc gccccgcgaa
     2641 ccccagaaaa gcgcgcggcg tcgtcccgca agtaagcgcg tgagggcttt ccgggcatga
     2701 agccgtcgcc cggaaccgac gccggcggcg gatacggggc gcgcgaggtg tcgcagatgc
     2761 tcggcctcac ggtcgggcag gtgcgtgcct gggtgcggtc ggggctggtg gatccggagc
     2821 gcggcccgcg aggagagttg cgcttctcgc cgcaggatct cctctttctt cgcgcggcgc
     2881 gaggccttgc tggcgcgcgg atcggaccgc ggcgaatcga gcgcgcgctc tcgaagctgc
     2941 gtgagcaggt ttccgcggcc gagtcgatcg gcgacctcga gatccgggcc gagggccgca
     3001 caatcgtcgt gaccgacggc tcggcgcgct ggcagcccga gtcggggcag atgctgatgg
     3061 agttcgagga aaagatcggt acctgtgttc cctttccacc gccctcactt cgcccctctt
     3121 ccggggtgag cgggagagag agcgcatccg agtggtacca gcgcggctgc gaactcgagg
     3181 agacggaccc ggacgcagcg cgtgaggctt atgcgaacgc gctgtcgttc gacgcgtcgc
     3241 acgcggacgc gcacgtcaac ctcgggcggc tcctccacga ggcgggagag gcggccgccg
     3301 ccgaggagca ttacaagagt gctctcgcct cgcgtccgga cgacgtcacg gccgcgttca
     3361 acctcggcgt cgcgctcgag gacctcggcc gcgacatcga ggcgatcgac gcctacgaga
     3421 aggcggtgtc gctcgacccg cagcactccg atgcctactt caacctcgcc ggcgcctgcg
     3481 agcgcgtcgg tcggccggcg gcggcgatcc ggcacctgaa ggcctaccgc gcgctcgtcc
     3541 gctcgcagtg acggtcgacc gcggcgccac gcccgcggac cccgcggaga aggggaaatc
     3601 caaccgccgg cgtgccgggc gcaggccggg cggcttctcg ctcctcacgg ccgacgacct
     3661 ccacctcttc aacgagggca cgcacacgcg gctgtgggag aggctcggcg cgcatcccgc
     3721 gaccgtcgcg gggagagccg ggacgaattt cgccacgttt gccccggaag cgcaggcggt
     3781 ttccgtcgtt ggagacttca acggctggaa cccggacacc catccgctcg aatcgcgagg
     3841 ccgctccggt ttgtgggagg gtttcgtgcc ggaagtgggg accggggcgc tgtacaagtt
     3901 ccacgtccgg tcgagacacg ccggctaccg cgtcgacaag acggatccct tcgccttttc
     3961 ccgcgagacg ccgccgaaga ctggctccat cgtgtgggac ctctcgtacg agtggacgga
     4021 ctcgggatgg atggaggatc gcgcgaagcg atccgcgcgc gatcggccga tgtcgattta
     4081 cgaggttcac ctcggatcgt ggatgcggcc cgacgggcgc ctccccggtt atcgcgagat
     4141 cgcgccggtt ctctgcgagt acgcaaagag catgggtttc acccacgtcg agctgctgcc
     4201 cgtgatggag caccccttct acggatcgtg gggataccag acgaccgggt ttttcgcgcc
     4261 cacgagccgc tacggcgggc cgcaggacct gatgttcctg atcgaccagc tgcacgagaa
     4321 cgggatcggc atcatcttcg actgggtgcc ctcgcacttc ccgtccgacg aacacgggct
     4381 cgtcttcttc gacggcagct acgtctacga gcacgccgac ccgcggcaag cggtgcagcc
     4441 ggactggggc agcctcgtct tcaactacgg ccgg
//
LOCUS       DASWAV010000021         3532 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U2665
            k141_481246, whole genome shotgun sequence.
ACCESSION   DASWAV010000021 DASWAV010000000
VERSION     DASWAV010000021.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960316
            Sequence Read Archive: SRR10260007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 3532)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3532)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:47:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,929
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3532
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_481246"
                     /isolate="SMAG_U2665"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.36444 N 132.31889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(223..921)
                     /locus_tag="VF958_00430"
     CDS             complement(223..921)
                     /locus_tag="VF958_00430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012844915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015232 - heme transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015886 - heme transport [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmB"
                     /protein_id="HEX9147599.1"
                     /translation="MKRETEPARLGFVSRALAVCGKDAAQELRRRVAIASVFFFAATA
                     IALVSYAVGPFGIPPEARAGLDASLLWILLFFSAATGLPRAFVREEETGTGLALRKLA
                     PGVVVLAGKSLFNFVLFLAIAALSVPAFAILLEWQVGSPAALATVILLGGWGLSIVST
                     FLSALVARAGQRNVLFVVVSFPLLVPLLLPAIAATIEASQGTFAATPLRVLVAYDGAA
                     TSAAYLLASAAWED"
     gene            complement(918..1541)
                     /locus_tag="VF958_00435"
     CDS             complement(918..1541)
                     /locus_tag="VF958_00435"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX9147600.1"
                     /translation="MGSARIEATGLTKSFSGPPLFSDVSFTLDSGVLAVTGANGTGKT
                     TLLKILAGLIRPTSGSVRVERDGREFSADERRVGVGWSGPDLFFYPELTAAENLTFFR
                     RVAGFAEPGDIASRLAAVGLGSARGAVEHFSTGMKQRLRIAFTFLLDPAVVLLDEPLS
                     GLDAQGREAVAQFVAAAGRRGPVLLAGTDLSELPPPDQVLEIVRREP"
     gene            1730..2527
                     /locus_tag="VF958_00440"
     CDS             1730..2527
                     /locus_tag="VF958_00440"
                     /inference="COORDINATES: protein motif:HMM:NF025006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HEX9147601.1"
                     /translation="MPRRTLTLLLAFLVPLFFVACGGKKDEDALLEEPTPAAAPGGAA
                     PAAAPAAAPAGSATITGKVAFEGTAPTMEKIKMDADAYCKSAHAQAVYTQDVVVNSNN
                     TLQWVFVYVKEGISGNYPAPTTPVTIDQQGCQYHPHVFGIQAGQPLKILNSDSTLHNI
                     HALPKKNAEFNIGQPFKGMETVKKFDTAEVPVHFKCDVHKWMGAYAGVVNHPFFAVTN
                     EQGTFEIKNLPPGNYVIEAWQEKYGPQTQNVTISGSESKTVDFTFKG"
     gene            2535..3293
                     /gene="coxB"
                     /locus_tag="VF958_00445"
     CDS             2535..3293
                     /gene="coxB"
                     /locus_tag="VF958_00445"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR02866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit II"
                     /protein_id="HEX9147602.1"
                     /translation="MSREALPGRRIRIGLAALVAAAAPSLASALPIWMKLPENVSTYG
                     GQIDGLFRLILWITGVIFVVVEALLLFFLFRYRHREGRVPRYTHGSNRLEVIWTIVPA
                     AICVILALLSRRSWAEIKQNIPQQAMNIEITAEQFAWNIRYPGPDGKLDTSDDVVTLN
                     QLHFPVGRAVVVTLHSKDVIHSFFLPEFRVKQDAVPGMTTRIWFEATRVGHWEIACAE
                     LCGLGHFRMKGFVTVETPEEFDRWLAETAREASS"
     gene            3290..>3532
                     /locus_tag="VF958_00450"
     CDS             3290..>3532
                     /locus_tag="VF958_00450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020382257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome-c oxidase"
                     /protein_id="HEX9147603.1"
                     /translation="MSSTIAVAHELPPGVHAAPTSFWRKYIFSLDHKVIGKQYLFYAL
                     FMLVIGGLLATLVRWQLAWPGRPIGFMGKLAPEGMPN"
BASE COUNT          664 a         1169 c         1165 g          534 t
ORIGIN      
        1 gcgagcgccg ccgtccacgc cgccgggacg tgaaagaaca cgatcctcga agactccccg
       61 aggaatccct tcgccggttc cacccagagg aaggcaccgg cgatcaccgc cgccatgtag
      121 gggagcagga ggagcttcaa gatcttcatc ggtcctcgtt catcttttct cgactgcggc
      181 gggtgcaggt tcgcggaacc cgtccccgcg acattcccag ggtcaatctt cccacgccgc
      241 agacgccagg agataggcgg ccgaggtcgc ggccccgtca taggcgacca ggacgcggag
      301 cggggtggcg gcgaacgtgc cctgcgacgc ctcgatggtc gcggcgatcg ccgggaggag
      361 gagaggcacg agcagcggga acgagacgac gacgaagagc acgttgcgct ggccggcccg
      421 agccacgagc gccgagagga aggtcgaaac gatcgagaga ccccagccgc cgagaaggat
      481 caccgtcgcg agcgcggcgg gggagccgac ctgccactcg agaaggatcg cgaacgcggg
      541 aaccgagagc gccgcgatcg cgaggaagag gacgaagttg aacagggact tgccggcgag
      601 cacgacgacg cccggggcga gcttgcgcag agcgagcccc gtcccggtct cttcttcccg
      661 cacgaatgcc cgcggcagac ccgtcgccgc cgagaagaag agcaggatcc agaggagcga
      721 agcgtcgagc ccggcccgcg cctccggggg gattccgaag ggtccgacgg cgtaggaaac
      781 gagcgcgatc gccgtcgcgg cgaagaagaa gaccgacgcg atcgcgaccc gccggcgaag
      841 ctcctgcgcg gcgtccttcc cgcagaccgc cagggcgcgc gagacgaacc cgagccgcgc
      901 cggctccgtc tcgcgtttca cggctcgcgt ctcacgatct ccagcacctg gtcgggcggc
      961 ggcagctcgg agaggtcggt gccggcgagg aggacgggtc cgcggcgtcc ggcggccgcg
     1021 acgaactggg ccaccgcttc ccgaccctgc gcgtccagtc ccgagagcgg ctcatccagc
     1081 aggaccaccg ccggatcgag cagaaacgtg aacgcgatcc ggagtcgctg tttcatcccg
     1141 gtggaaaagt gctcgactgc cccgcgggcc gaacccagtc cgaccgcggc gaggcgggag
     1201 gcgatgtcgc ccggctcggc aaagcccgcc acgcggcgga agaacgtcag gttctccgcc
     1261 gccgtcagct ccggatagaa aaagagatcc ggtccggacc agccgacccc gacccgacgt
     1321 tcgtccgccg agaactcgcg cccatcgcgc tcgacccgga cgcttcccga ggtcggccgg
     1381 atcaggccgg cgaggatctt caggagtgtg gtcttgccgg ttccgttggc gccggttacg
     1441 gcgagcacgc cgctgtcgag agtgaacgag acgtccgaga agagcggcgg tcccgagaac
     1501 gatttcgtga gaccggttgc ttcgattcgt gcagagccca ccctcgcgcc aagataccag
     1561 aggagcccga cggactcggc ttgaaatgcg ctgcgccgtt ctctatgatc cgcccggctt
     1621 tgggacagat ttgttttctc ggcgtcctct gcgaccgaca cggtcccgga acagacgact
     1681 tgcggcgcgt ccaatagaca gaatggatct gcccccgaag ggagtaacga tgcccaggcg
     1741 aactctcact ctgctcctcg cgtttctcgt gccgctcttc ttcgtcgcgt gcggcggaaa
     1801 gaaggacgag gacgctcttc tcgaagaacc aactcccgcc gccgcaccgg gaggggcagc
     1861 tccggccgcc gcccccgccg ccgcgcccgc cggcagtgcg acgatcacgg ggaaagtcgc
     1921 gttcgaggga acggccccga ccatggagaa gatcaagatg gacgcggacg cctactgcaa
     1981 atcggcgcac gcccaggcgg tctacacgca ggacgtcgtc gtcaattcga acaacaccct
     2041 gcagtgggtc ttcgtttacg tcaaggaagg catttcgggc aactatcccg cgccgacgac
     2101 gccggtgacg atcgaccagc agggttgcca gtatcacccg cacgtcttcg gcattcaggc
     2161 cgggcagccc ttgaagatcc tgaacagcga ctcgacgctc cacaacatcc atgcgctgcc
     2221 gaagaagaac gcagagttca acatcggaca gcccttcaag ggcatggaga ccgtcaagaa
     2281 gttcgacacc gcggaagtcc cggtccactt caagtgtgac gttcacaaat ggatgggcgc
     2341 ctacgccggc gtggtgaacc accccttctt cgcggtgacg aacgagcagg gaacgttcga
     2401 gatcaagaat cttccgcccg gcaactacgt catcgaggcc tggcaggaga agtacggccc
     2461 tcagacccag aacgtgacga tctccggatc ggagtccaag acggtcgact tcacgttcaa
     2521 gggttaacgg ccccgtgtca cgagaggcgc tgccgggccg gaggatccgg attggtctcg
     2581 ccgcgctcgt cgcggcggcg gctccctccc tcgcttcggc gctgccgatc tggatgaagc
     2641 ttccggaaaa cgtctcgacg tacggcggcc agatcgacgg cctcttccgg ctgatcctct
     2701 ggatcaccgg ggtcatcttc gtcgtcgtcg aggcgctgct tctcttcttc ctcttccggt
     2761 accggcaccg ggaggggaga gttccgcgct acacgcacgg ttcgaaccgg ctcgaggtga
     2821 tctggaccat cgtgcccgcg gcgatctgcg tgatcctcgc tctgctctcc cggcgcagct
     2881 gggcggagat caagcagaac attccgcagc aggcgatgaa catcgagatc accgcggagc
     2941 agttcgcgtg gaacatccgt taccccggtc cggacggcaa gctcgacacc tcggatgacg
     3001 tcgtcacgct gaaccagctc cacttcccgg tggggcgcgc ggtcgtcgtg acgctccact
     3061 cgaaggacgt cattcactcg ttcttcctgc cggagtttcg cgtgaagcag gacgcggtcc
     3121 cgggtatgac gacgcgcatc tggttcgagg ccacgcgcgt ggggcactgg gagatcgcgt
     3181 gcgcggagtt gtgcggcctc ggtcacttcc ggatgaaagg gttcgtcacg gtggaaacgc
     3241 ccgaggagtt cgaccggtgg ctggcggaga ccgccaggga ggcaagctca tgagcagcac
     3301 gatcgccgtc gcgcacgaac tcccgcccgg agtccacgcg gcgccgacat cgttctggcg
     3361 caagtacatc ttctcgctgg accacaaggt gatcgggaag cagtacctct tctatgccct
     3421 cttcatgctg gtgatcggag ggcttctcgc gacactcgtg cggtggcagc tcgcctggcc
     3481 cggcaggccg atcggattca tgggcaagct cgcgcccgag ggaatgccga ac
//
LOCUS       DASWAV010000022         3727 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U2665
            k141_80916, whole genome shotgun sequence.
ACCESSION   DASWAV010000022 DASWAV010000000
VERSION     DASWAV010000022.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960316
            Sequence Read Archive: SRR10260007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 3727)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3727)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:47:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,929
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3727
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_80916"
                     /isolate="SMAG_U2665"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.36444 N 132.31889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            72..959
                     /locus_tag="VF958_00455"
     CDS             72..959
                     /locus_tag="VF958_00455"
                     /inference="COORDINATES: protein motif:HMM:NF014477.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HEX9147604.1"
                     /translation="MLLLAASHGSGGSRVTRVRWEMGTLLEVDAIGPERNRTEQAVAA
                     AFAAVEEVDRRLSNWKPDSELSLVNAASGGFVSLSPATWRSLSLAIALARETGGAFDP
                     TVGAFTGAPTVRWENVMLDPGERTLTFRGGARGAIDSGGFGKGEALDRALVELRRRGV
                     TAARLNFGGQISAFGGGSFAEVSIAEPRGGSGRELCSFTPVDGSVSTSGVSEKPGHIV
                     DPRTDRAAAFTGSVTVVADTGVRADALSTALFVLGPEAGLDFANRRGIAALFAASRPG
                     GGWQLLASRRFPQFFSKAS"
     gene            969..2345
                     /locus_tag="VF958_00460"
     CDS             969..2345
                     /locus_tag="VF958_00460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9147605.1"
                     /translation="MRRFATEFLFLFFAIALALPAQQSADRVRELEKKVEELEHRLDA
                     LTAGADEERRRQIEELRRQIDVLTREIENLKSAAPEKATPAGERGAFGLGPAASKVYG
                     STRGVSIGGYGEVLYQNFATERENGSKSGRTDTIDLARAVFYFGYKFDDHFLFNSEIE
                     YEHATTGEGAEEKGEVSVEFAYLDYLARPEVNVRAGLLLLPLGFVNELHEPPVFLGAR
                     RPEVERRIIPATWRELGIGVFGDAGPFAYRAYLVNGLDAGGLDAASPLREARQGGAEA
                     RAEDFAVTARLDFTGVPGLLVGAAGYSGGSAQGREVAGRSFDARVTVLDAHAEWRWRG
                     IQARALAVRGTIGDAADVNRSNGFTNEDSVGRRFTGTYVEAGYDVLFGRASGSLVPFL
                     RWERFDTQDEVPAGFASDPANDVRLWTVGLQYRPIPQVVVKLDYQDAHDQARTGVNQW
                     NLALGWLF"
     gene            2423..2911
                     /locus_tag="VF958_00465"
     CDS             2423..2911
                     /locus_tag="VF958_00465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding protein"
                     /protein_id="HEX9147606.1"
                     /translation="MLALPVYSSREEALARAFPPPATIERRTYFLTDEEREKASHAAR
                     TKVESALVIAYVAKGPNGPVGTGYFDTHVVRTMPETLLVTVRPDGSLQAVEVVAFGEP
                     EDYLPRRNWLKLFPGRRLDDELTVGRALAHVSGATLTTRVIADAARRVLAIHAVVAGR
                     TQ"
     gene            2908..3426
                     /locus_tag="VF958_00470"
     CDS             2908..3426
                     /locus_tag="VF958_00470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9147607.1"
                     /translation="MKYMERGGFQGQPLMRLTLGLSILLLALFWVTNFALFASRLGFT
                     PASVAAYYVGSEADYRPPRTAGSMLEVTHAHLPMMALVLLILTHLLIFASYSKRTRVL
                     LIVGAFLSAALDEGAGWLVRFVHPGFAVVKLAAFASLQGILAFLLVALALFLRAGARA
                     EKGNGLRVTPSR"
     gene            complement(3410..>3727)
                     /locus_tag="VF958_00475"
     CDS             complement(3410..>3727)
                     /locus_tag="VF958_00475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9147608.1"
                     /translation="FHDAEAADRAEGDFRRAFSKGELPEKIETQEVAAASHGTAARAL
                     VAVGLAGSMREARRKIAEGALSVYGPGGASRPIRDPEERLASPEPTVVRLGRRFVRII
                     WKG"
BASE COUNT          508 a         1264 c         1307 g          648 t
ORIGIN      
        1 tccagcaaat gtcgcgcctt ggcacactat tgagactgag tctctgtctt atcggaggcg
       61 gtgcgactgt ggtgctgctc ctcgccgcat cccacgggag cggtggatcc cgcgtcaccc
      121 gtgtgcggtg ggagatgggc acgcttctcg aggtcgacgc gatcggtccg gagcggaatc
      181 gcacggagca ggcggtggcc gccgcctttg cggccgtcga agaggtcgac cggcgactgt
      241 ccaactggaa gccggactcg gagctctcgc tggtcaacgc ggcgtcggga gggttcgtat
      301 cgctgtcgcc ggcgacgtgg cgcagcctct ctctcgcgat tgcactcgcc cgcgagaccg
      361 gtggcgcgtt cgatccgacg gtcggcgcgt tcaccggcgc gcccacggtg cgctgggaga
      421 acgtgatgct cgatccaggg gagcggacgc tgacctttcg cggaggcgcg cgcggggcca
      481 tcgactccgg cggcttcggg aagggcgagg cgctcgaccg agctctcgtc gagcttcggc
      541 ggcggggagt gaccgccgcg cggctcaact tcggcggtca gatctcggcg ttcggcggcg
      601 gcagctttgc ggaagtctcc atcgccgagc cccgcggcgg atcggggcgg gagctctgct
      661 cgttcactcc ggtcgacggc tctgtctcga cttccggcgt ctcggagaag cccggccaca
      721 tcgtcgatcc gcgcacggac cgggccgccg ccttcacggg gtccgtgacg gtcgtcgccg
      781 ataccggcgt gcgggcggac gctctttcga cggcgctttt cgtgctcgga cccgaggccg
      841 gcctcgattt cgcgaaccgg cgcgggatcg cggcgctttt cgcggcttcc cgccccggcg
      901 gcgggtggca actcctcgcg agccgccgct ttccgcagtt cttctcgaaa gcatcctgag
      961 tcttccgatt gcgacgtttt gcgacggagt tccttttcct cttcttcgcg atcgctctcg
     1021 ctttgccggc gcagcagtcg gcggaccgtg tgcgcgagct cgagaagaag gtcgaggagc
     1081 tggagcatcg gctcgatgcg cttaccgcgg gcgcggacga ggagaggcgc cggcagatcg
     1141 aggagctccg ccggcagatc gacgtgttga cgcgcgagat cgagaacctg aagtccgccg
     1201 cgccggaaaa ggcgaccccg gccggagagc gcggcgcgtt cggtctgggg ccggcggcgt
     1261 cgaaagtcta cggctcgacc cgcggcgtct cgatcggcgg gtacggcgag gtcctgtacc
     1321 agaacttcgc cactgaacgg gagaacggct cgaagtccgg ccggacggac acgatcgacc
     1381 tcgcgcgggc ggtcttctac ttcggctaca agttcgacga ccacttcctc ttcaattcgg
     1441 agatcgagta cgagcacgcg accaccggtg aaggcgccga agagaagggc gaggtttcgg
     1501 tcgagttcgc ttatctcgat tacctcgctc gcccggaagt gaacgtccgt gccggactcc
     1561 ttctcctccc gctcggattc gtgaacgagc tgcacgagcc cccggtcttt ctcggcgcgc
     1621 gccggcccga ggtcgagcga cgcatcattc cggcgacgtg gcgcgagctc gggatcggag
     1681 tcttcgggga cgcgggaccg ttcgcttatc gcgcgtatct cgtcaacggg ctcgatgccg
     1741 gaggcctcga cgctgcgtcg ccgctgcgcg aagcccgtca gggcggggcg gaggcgagag
     1801 ccgaggactt cgccgtgacc gcacgcctcg acttcaccgg agtccccggt ctcctcgtcg
     1861 gggcggccgg atactccggc ggatcggcgc agggccgaga agtcgcgggg cggtcgttcg
     1921 acgctcgcgt cacggttctc gacgctcacg ccgagtggcg ctggcgcggg atccaggccc
     1981 gagccctcgc cgtgcggggg acgatcggcg acgcggccga cgtcaatcgc tccaatggct
     2041 tcacgaatga ggactccgtg ggccggcgat tcacggggac ttacgtcgaa gcgggatacg
     2101 acgtgctgtt cggccgcgcc tccggatcgc tcgtgccgtt cctccgatgg gagcgcttcg
     2161 acacgcagga cgaggtcccg gcgggattcg cgtccgatcc cgcaaatgac gtgcggctgt
     2221 ggacggtcgg cctccagtac cgcccgatcc cgcaggtcgt cgtcaagctc gattaccagg
     2281 acgcgcacga ccaggcacgc actggtgtca accagtggaa cctcgcgctc gggtggctgt
     2341 tctgaccggg tggcgggcgg gcgcagctcc ctcaccccgt ccctctcccg gtgggagagg
     2401 gagttttctc gccgcgctcg ttttgctcgc tcttcccgtt tactcctcgc gagaggaggc
     2461 gctcgcccgc gccttcccgc cgccggcgac gatcgagcgc aggacgtatt tcctgaccga
     2521 tgaggagcgc gagaaggcga gccacgctgc ccggacgaaa gtcgaatcgg ctctcgtcat
     2581 cgcgtacgtc gcaaaggggc cgaatgggcc ggtcgggacc ggctacttcg acacgcacgt
     2641 cgtccgcacc atgccggaga cgcttctcgt cacggtccgg cccgacggct ctctccaggc
     2701 cgtggaggtc gtcgcgttcg gcgagccgga ggactacctg ccgcgccgta actggctgaa
     2761 gctctttccc gggcggcggc tggacgacga gctcaccgtg ggccgggcgc tcgcgcacgt
     2821 cagcggggcg actctcacga cgcgggtcat cgccgatgcg gcgcgccggg tgctcgcgat
     2881 ccacgcggtg gtcgccgggc ggacgcagtg aagtacatgg agcgcggcgg ttttcagggg
     2941 cagccgctca tgcgcctgac gctcggcctg tcgatcctgc tgctcgcgct cttctgggtg
     3001 acgaacttcg cgctgttcgc atcgcgactg ggcttcacgc cggcgtcggt cgcggcttat
     3061 tacgtcggct ccgaggcgga ctaccgacct ccccggacgg ccggctcgat gctggaggtg
     3121 acacacgcgc accttccgat gatggcgctc gtcctgctga tcctgacaca tctcctgatc
     3181 ttcgcgtcgt actcgaagcg gacgcgcgtg ctgttgatcg tcggcgcctt cctctccgcc
     3241 gcgctcgacg agggagcggg atggctggtc cgtttcgtcc atccgggctt tgcggtcgtc
     3301 aagctcgcgg cgttcgcttc gctgcagggg atcctcgcgt tcctgctcgt ggctctggcg
     3361 ctcttccttc gggccggcgc ccgggccgag aaagggaacg ggctgcgagt caccccttcc
     3421 agatgatccg gacgaagcgc cttccgagcc gcacaacggt cggctcggga gacgcgagac
     3481 gctcctcggg atcgcgaatc ggacgggacg cgccgccggg cccgtagacg gagagcgcgc
     3541 cttcggcgat ctttctgcgc gcctcgcgca tcgagccggc gagtccgacc gcgacgagcg
     3601 cgcgcgcggc cgttccatgg gacgccgccg cgacctcctg cgtctcgatc ttctcgggca
     3661 actcgccctt cgaaaacgcg cgccggaagt ccccttcggc gcggtccgcc gcctccgcgt
     3721 cgtggaa
//
LOCUS       DASWAV010000023         1514 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U2665
            k141_321416, whole genome shotgun sequence.
ACCESSION   DASWAV010000023 DASWAV010000000
VERSION     DASWAV010000023.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960316
            Sequence Read Archive: SRR10260007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 1514)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1514)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:47:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,929
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1514
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_321416"
                     /isolate="SMAG_U2665"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.36444 N 132.31889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1514)
                     /locus_tag="VF958_00480"
     CDS             complement(<1..>1514)
                     /locus_tag="VF958_00480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9147609.1"
                     /translation="VGWRPDRPIEVVIGDPQATSNGMAFPFLDRPAVVLWASPPEPES
                     GIGFWTDWMEDLVVHEVAHIVHLGRPRNRSRGVLARLSPLPIGPVLLNSPRWLSEGYA
                     TLIEGELTGSGRPASSFRAMVLRRFAMDGKLPGYATLSSTSGWLGGSMAYLVGSTFLE
                     WLEAKEGKDTLPKLWRRMASRRGGNFPTAFRAVFGRSPSDLYDRFRAELTGGALEEEK
                     RLRAAGLVDGDTWLRLDGGTLSLEVSPDGSKLLARRDPKRGETYLAVWTIAKREEERR
                     AEEKRREREKEILRDPEEVPDKPELPPRRAPRWRLPSVNGYSAENPRWTPDGRVLFVR
                     RAPDADGVLRLDLYRWTPETGAIERLTELADVTEADPAPDGRWAAGVRVRYGISELVT
                     VDLSSGEVRPIAPAPASEDAWRVWSHPRLSPDGTTIAVLLHTGGRWRLVTTPAAGGEL
                     REIALPGSPAGALSWSADGSRIYVAADREGIWNLESVDARGEEAPELLTRVTGG"
BASE COUNT          226 a          548 c          509 g          231 t
ORIGIN      
        1 cgccgcccgt gacgcgtgtc agaagttccg gcgcttcttc cccccgcgca tcgaccgact
       61 ccaggttcca gatgccttcc cgatccgcgg cgacgtagat ccgcgacccg tcggcgctcc
      121 atgaaagtgc gccggccggc gatccgggaa gagcgatttc gcggagttcg ccgccggccg
      181 caggggtcgt cacgaggcgc cagcgtccgc cggtgtggag gagtacggca atggtcgtcc
      241 cgtccggcga caatcgcggg tgactccata cccgccacgc gtcctcgctc gctggcgccg
      301 gcgcaatcgg ccggacttct ccggaggaga gatccaccgt cacgagctcc gagattccgt
      361 agcgcacgcg gactcctgcc gcccaacggc cgtcgggcgc gggatcggct tcggtcacgt
      421 ccgcgagctc ggtcagccgc tcgatcgcgc ccgtctccgg cgtccagcgg tacaggtcga
      481 gcctgaggac gccatccgca tccggcgcgc gccgcacgaa gagcacgcgg ccgtccggcg
      541 tccatcgcgg gttctcggcg gagtagccgt tcacgctcgg cagtcgccaa cggggcgcgc
      601 ggcggggagg gagttccggt ttgtcgggga cttcttccgg atcccggaga atctccttct
      661 cgcgttcgcg acgtttctct tcggcgcgcc gctcttcctc gcgcttggca atcgtccata
      721 cggcgaggta cgtctctccc cgtttcgggt cgcgtctcgc gaggagcttc gagccgtcgg
      781 gtgaaacctc gagcgagagt gttccgccgt cgagccgaag ccaggtgtcg ccgtccacga
      841 gtcccgccgc gcgcaggcgc ttttcctcct cgagggcgcc gccggtcaac tccgcgcgga
      901 agcggtcgta gaggtccgag ggagaacgcc cgaagaccgc gcggaatgcc gtcgggaagt
      961 ttcctccgcg ccgcgaggcc atccgcctcc agagtttcgg cagggtgtcc ttgccctcct
     1021 tcgcctccag ccactcgagg aacgtcgaac cgacgaggta cgccatcgag ccgccgagcc
     1081 agcccgacgt cgaggagagc gtcgcgtagc cgggaagctt cccgtccatc gcaaacctcc
     1141 gcagcaccat tgcgcggaaa ctcgacgccg gacgtccgga gcccgtcagc tcgccttcga
     1201 tcagcgtcgc gtacccttcc gacagccagc gcggcgagtt cagcagaacc ggcccgatcg
     1261 gcagcggcga gagtcgcgcg aggactccgc gcgagcggtt gcgcggccgg ccgaggtgaa
     1321 cgatgtgcgc gacctcgtgc acgacgaggt cctccatcca gtccgtccag aacccgatgc
     1381 cggactcggg ctcgggggga gaggcccaga gcacgaccgc gggccggtcg aggaacggaa
     1441 acgccatccc gttcgacgtt gcctgcggat cgccgatcac gacctcgatc ggacggtccg
     1501 gccgccagcc gacg
//
LOCUS       DASWAV010000024         4165 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U2665
            k141_521205, whole genome shotgun sequence.
ACCESSION   DASWAV010000024 DASWAV010000000
VERSION     DASWAV010000024.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960316
            Sequence Read Archive: SRR10260007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 4165)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4165)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:47:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,929
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4165
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_521205"
                     /isolate="SMAG_U2665"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.36444 N 132.31889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            145..1560
                     /locus_tag="VF958_00485"
     CDS             145..1560
                     /locus_tag="VF958_00485"
                     /EC_number="2.7.8.31"
                     /inference="COORDINATES: protein motif:HMM:TIGR03023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-phosphate glucose
                     phosphotransferase"
                     /protein_id="HEX9147610.1"
                     /translation="MRPGDIHRSNRRLAALFLASDLAATFVALTGAYWLRFEAEIIPV
                     TKGVPPASSYLRLFPFVAVLWPLVYYFHGLYQVRRNRSSIEEGFAVLVATALATLLFI
                     GITTFSRGFDYSRAVLALFFASDVLLVFAGRTAIRRYLEEAWRHGYGVRRVLVVGAGK
                     LGRAVVDKLAEHPEAGLRAVVFVDDDPEKNGLAYRGVAIVGRTADAARVVEERDVDAV
                     FLALPLEAHRAMLDVLKDVARTVADVRVVPDLLQYITFRAGVEDLDGLPVVHLTQVPL
                     TGWMSLVKRTLDLAIAATALVLLSPLFAVIAVAIRLSDGPRVFYRQRRMGLDGRPFEI
                     VKFRSMRHDAEDELGPTWAAPEDPRRTRVGRFLRRWSLDELPQLVNVVKGEMSLVGPR
                     PERPEFVREFKEKFPQYMLRHRVRAGITGWAQVNGWRGATDLAKRIEYDLYYIENWSL
                     ALDVKILWLTVRYGLRHRNAY"
     gene            complement(1557..3002)
                     /locus_tag="VF958_00490"
     CDS             complement(1557..3002)
                     /locus_tag="VF958_00490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9147611.1"
                     /translation="MTPLVLSILVPMPLVAGSDVPLVNWTVPPYHHASARRGLSTMTD
                     VSPGVAFTAIQPCRVVDTRNPVGPYGGPALPTAGVRTFDVDSGPCPGIPQGVDAYSLS
                     IGAILPATDGYLTAWANGTPRPAVSQVNYLAGEVIANAAIVPAGTSGSIDVFVSSGPT
                     NVYVDINGYFTDSYNPGQSFVARSSTFNPAIVAYNSSTADFADAIYGELTSTACGIDS
                     AALSGYNNTANANGFGVWGGHAGQGSGVYGTSPFGTGVHGVSRATAVEGRLVDPLGAP
                     IAQGRLGVDFGTNAPPWAVYAQGDIGASGTKNFRDPHPTDASLVISYTSLEGPEAGTY
                     FRGRGRFENGLARIVVPEDFRMVTAPEGLTVQVTPIGGMASVGVLRMDLNEIVVQSSR
                     NLDFSYLVQGVRASFPDEHPIVAGQDFVPPSAYAVIPGWLSQRQKRLLVENGTYNADG
                     TVNMETARRLGWDRKWGVREQPVETRPEAGR"
     gene            3206..>4165
                     /locus_tag="VF958_00495"
     CDS             3206..>4165
                     /locus_tag="VF958_00495"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="sialidase family protein"
                     /protein_id="HEX9147612.1"
                     /translation="MTPNLSPDGGTVLLSWLEPPHSRVKPQDGNYALRFSRLVNGQWT
                     EPVTIASGTDFFANWADFPSVTAGARGRMVAHWAAKSGADTYAYDVRLARSDDGGKTW
                     RPIGPAHDDRTPTEHGFVSAVPEGDRVRIFWLDGRETGGGKGSMTLRTALVGDHVGKA
                     DLLDSRVCDCCQTAAAVTSSGPIVAYRDRSEDEIRDVVVVRRNADRWTAPRPVSNEGW
                     KIEGCPVNGPAIAASGRRVAVAWFTQGGNRPRVQVAFSEDAGASFAPPVVVDRNGPLG
                     RVDVVLDENGDALVLWVASEGKAAAIRLARVTPAGKVGTALAVS"
BASE COUNT          631 a         1423 c         1424 g          687 t
ORIGIN      
        1 gatcgagaag gccgggcgcg cgctcttcga ttacactgcc gttcgcgcga cggcactgcc
       61 gttcgcgcgc gagcgattcg tccgggagtt ccgacgagcg atctctgaag tgcagcagac
      121 caaccacatc cgggagccgc gcccgtgagg cctggcgaca tccaccgatc caaccgccgc
      181 ctcgcggcgc tcttcctcgc gtccgacctc gcggcgacct tcgtggcact caccggcgcc
      241 tactggctcc gtttcgaggc cgagatcatc cccgtcacga agggcgtgcc gccggcctcg
      301 tcctacctgc gcctctttcc cttcgtcgcc gtgctctggc cgctcgtcta ctactttcac
      361 ggcctctatc aggtccggcg caaccgctcg agcatcgagg agggctttgc ggttctcgtg
      421 gcgaccgcgc tcgcgaccct gctcttcatc gggatcacga cgttctcgcg gggattcgac
      481 tactcgcgcg cagttctcgc gctcttcttc gcgtcggacg tcctgctggt cttcgcggga
      541 cggaccgcga tccgccggta cctggaagag gcgtggcgcc acggctacgg cgtgcggcgg
      601 gtgctcgtcg tcggagccgg gaagctcggt cgggcggtcg tcgacaagct cgccgagcac
      661 ccggaggcgg gtctgcgcgc cgtggtgttc gtcgacgacg atccggaaaa gaacggactg
      721 gcgtatcgcg gagtcgcgat cgtcggccga acggcggatg ccgcccgagt cgtcgaagag
      781 cgcgacgtcg acgccgtctt cctcgcgctg ccgctggagg cgcaccgcgc gatgctcgac
      841 gtgttgaagg acgtcgcccg gacggtcgcc gacgtccgag tggtgccgga cctccttcaa
      901 tacatcacgt tccgcgcggg cgtcgaggat ctggacggtc ttccggtggt tcacctgacg
      961 caggttccgc tgaccggctg gatgagcctc gtcaagcgaa ccctggacct cgcgatcgcc
     1021 gcaactgcgc tcgtcctgct gtcgccgctc ttcgccgtga tcgcggtcgc aatccgcctc
     1081 tcggacggac ctcgcgtgtt ctatcgccag cggcggatgg ggctggacgg ccgcccgttc
     1141 gagatcgtga agtttcgctc gatgcggcac gacgccgagg acgaactcgg tccgacatgg
     1201 gccgcgccgg aagaccccag gcgcacccgc gtcggacgct tcctgcggcg ttggagcctg
     1261 gacgagctcc cccagctcgt caatgtcgtc aaaggcgaaa tgtcgctcgt cggcccgcgc
     1321 cccgagcgac ccgagttcgt tcgcgagttc aaggagaagt tcccgcagta catgctgcgt
     1381 caccgcgtcc gcgccgggat caccggctgg gcccaggtca acgggtggcg cggcgcgacg
     1441 gatctcgcaa agcggatcga gtacgacctc tactacatcg agaactggtc gctcgcgctc
     1501 gacgtcaaga ttctgtggct gaccgtccgg tacggtctcc ggcaccggaa cgcgtactag
     1561 cgtccggcct cgggacgcgt ttcgaccggc tgctccctca ctccccactt gcgatcccag
     1621 ccgagcctcc gcgccgtctc catgttgacg gtgccgtcgg cgttgtaggt gccgttctcg
     1681 acgaggagcc gcttctggcg ttgcgaaagc cagcccggta tcacggcata ggccgacggg
     1741 ggcacgaaat cctgcccggc gacgatcggg tgttcgtccg ggaagctcgc gcggactccc
     1801 tgcacgagat aggagaagtc gagattccgg gaggactgga cgacgatctc gttcagatcc
     1861 atccggagga cgccgaccga tgccattcct ccgatgggcg tgacctgcac agtgagacct
     1921 tccggggccg tgaccatccg gaagtcctcg ggtacgacga tccgggcgag tccgttctcg
     1981 aaccggccgc gtccgcggaa atatgttccg gcctctggcc cctcgagcga cgtgtaggag
     2041 atgacgagcg acgcgtcggt cgggtgcgga tcacggaagt tcttcgtgcc gctcgcgccg
     2101 atgtcgccct gcgcgtagac ggcccacggc ggcgcgttgg ttccgaagtc cacgccgagg
     2161 cggccctgcg cgatcggcgc gccgaggggg tcgacgaggc gtccttccac cgccgtagcc
     2221 cggctgacgc cgtgcacgcc ggttccgaaa ggggaggtcc cgtagacgcc cgacccctgc
     2281 ccggcgtgcc ctccccagac cccgaagccg ttcgcgttcg ccgtgttgtt gtacccggac
     2341 agggcggcgg aatcgattcc gcatgccgtc gaggtgagct caccgtagat cgcgtccgcg
     2401 aagtcggcgg tcgaggagtt gtatgcgacg atcgccggat tgaaggtcga gctccgcgcc
     2461 acgaagctct ggccagggtt gtaggagtcc gtgaagtacc cgttgatgtc gacgtagacg
     2521 ttcgtcggcc cgctgcttac gaagacgtcg atcgagcccg aagttcccgc cgggacgatg
     2581 gcggcgttcg cgatgacctc acccgcgagg tagttcacct gcgaaaccgc cggtcgaggg
     2641 gtcccgttcg cccaggcggt cagataaccg tcggtggccg ggagaatcgc tccgatcgag
     2701 agcgaatacg cgtcgacccc ctgggggatc ccgggacacg gaccgctgtc gacgtcgaag
     2761 gtgcggacgc cggcggtcgg caacgccggc ccgccgtacg gcccgacggg gttccgcgtg
     2821 tcgacgaccc ggcacggctg gatcgccgtg aacgcgacgc ccggcgagac gtcggtcatc
     2881 gtcgagagtc cccggcgcgc ggatgcgtgg tggtacggcg gcaccgtcca gttgacgagc
     2941 gggacatcgg agccggccac gagaggcatc ggcacgagaa tcgagagcac gagaggagtc
     3001 acaccaatcc tccgggaaaa gggcgccatg gagacctcct gaagggatgg tactgccgcg
     3061 tcgcgcctcg agttccggag ataatccggg cgtgccaggc gttgcggagc gttcggcgtt
     3121 tctcctcgcg tgcgcgttgg ccggcacact ctcagcggcg gagccgcgcg tcacgagtct
     3181 ggatcccccc gcgaagcccg gctcgatgac acccaacctc tcgcccgacg gcggcaccgt
     3241 gctcctctcc tggctcgaac cgccccactc tcgcgtcaaa cctcaggacg gcaactacgc
     3301 ccttcgcttc tcccgactgg tgaacggaca gtggaccgaa cccgtgacga tcgcctccgg
     3361 caccgacttc ttcgcaaact gggcggactt cccttccgtg accgcggggg ctcgcggacg
     3421 gatggtcgct cactgggccg cgaagtcggg cgccgatacg tacgcgtacg acgtgcgcct
     3481 cgcccggtcg gacgacggcg gaaagacgtg gcggccgatc ggacccgcgc acgacgaccg
     3541 gacgccgacg gagcacggct tcgtgtctgc cgttcccgaa ggcgatcgag tccggatctt
     3601 ctggctcgat ggccgtgaga cgggcggtgg aaagggatcg atgactctgc ggaccgcgct
     3661 cgtcggggac cacgtcggga aggccgacct cctcgactcg cgggtctgcg actgttgcca
     3721 gaccgccgcg gcagtgacct cgagcggacc gatcgtggcc taccgggatc gttccgaaga
     3781 cgagatccgg gacgtcgtcg tcgtgcgccg caacgccgac cggtggactg cgcccagacc
     3841 cgtctcgaac gaggggtgga aaattgaggg atgccccgtg aacgggccgg cgatcgcggc
     3901 cagcgggcgc cgcgtcgccg tcgcctggtt cacacaggga gggaaccggc cgcgggtgca
     3961 ggtcgccttc tcggaggatg ctggcgcctc gttcgctccc cccgtcgtgg tggaccgcaa
     4021 cgggccgctc ggccgggtcg acgtcgttct cgacgaaaac ggcgacgctc tcgtcctgtg
     4081 ggtcgcatcc gaaggaaagg ccgcggcgat ccggctggcc cgcgtgactc ccgctgggaa
     4141 ggtcggcacc gccctggcgg tctcg
//
LOCUS       DASWAV010000025         5511 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U2665
            k141_761272, whole genome shotgun sequence.
ACCESSION   DASWAV010000025 DASWAV010000000
VERSION     DASWAV010000025.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960316
            Sequence Read Archive: SRR10260007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 5511)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5511)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:47:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,929
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5511
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_761272"
                     /isolate="SMAG_U2665"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.36444 N 132.31889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..703)
                     /locus_tag="VF958_00500"
     CDS             complement(<1..703)
                     /locus_tag="VF958_00500"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: protein motif:HMM:NF013222.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MGMT family protein"
                     /protein_id="HEX9147613.1"
                     /translation="MKRIERASVNGTIAIVRGLKGLPRERAPKSLLPEVLRRVGLGDS
                     YWRLETSIGPVFVAHSRAGISMVTRARSATEFAKTFEKRFGRPIAPDATAPPRNVREL
                     AEGRLRARDAGLRFDLRGLSEFEQAVLRKALEIPPGEVRPYSWIAREIGHPDAVRATG
                     SALAKNPVPLLIPCHRVVRSDGHIGNYSLGGARNKRTLLESEGAQPETLEKLAAEGIR
                     FFGNEKERYFCFPTCG"
     gene            complement(700..1308)
                     /locus_tag="VF958_00505"
     CDS             complement(700..1308)
                     /locus_tag="VF958_00505"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEX9147614.1"
                     /translation="MAQRELVNALSEDLDGTFERLVRVFQDRLYAFAHRICASREDAQ
                     EVVQDAFVRAYRALKSYPPERVRALALQAWLYQITLNVARNRMRKKRPPHVSIDNGGA
                     NGAAAQKAWDSPDDPMARPDSRFERGQQRADIASLVATLPERYRSAIILRYVEGLSLE
                     EVADILKQPLGTAKSNVHRAVNMLRRAITDSRRVEGRAEVRA"
     gene            complement(1478..2464)
                     /locus_tag="VF958_00510"
     CDS             complement(1478..2464)
                     /locus_tag="VF958_00510"
                     /inference="COORDINATES: protein motif:HMM:TIGR04018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YpdA family putative bacillithiol disulfide
                     reductase"
                     /protein_id="HEX9147615.1"
                     /translation="MEPVAIVGAGPTGIACAIELGNRGIPAVCYDRGHLLHSIYHFPE
                     EMRWFSTRDLLDIAGVPFGVPDAHPTRLETLAYYRAVAEKFGVRVVPRTEIVATTAVP
                     SGGVEVSAVDAGGPRRFPAPAGILAPGFFDNPRRLGVPGEDLPHVHRRFVSGYPFHGR
                     DVVVIGGKNSASEAALDLYRHGARVTMIVRGPAISDRVKYWIKPDLENRISAGAIRAH
                     FEARAVEITPELVRVETPAGPESVAATAVFPLIGYEPDFALLERCGIRVEGPERAPAH
                     DPETLESNVPNLYLAGAILGGTEIGRIFIENSRHHAVWIAGAIERRRKYVPA"
     gene            complement(2536..3498)
                     /locus_tag="VF958_00515"
     CDS             complement(2536..3498)
                     /locus_tag="VF958_00515"
                     /inference="COORDINATES: protein motif:HMM:NF024743.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4097 family beta strand repeat-containing
                     protein"
                     /protein_id="HEX9147616.1"
                     /translation="MRVRLAPVLPVVLAGLFVPALSLSAGTRLEKALRLEPGGRFSLD
                     TDMGSVTVTGTAAPGARLVITSKRRDLDELLRFNFREDAGSVSVTARRRHGRIFGWFN
                     SGGDSVHWEVQVPEHTRVDIDTSGGAIKIASLQSDARLHTSGGGIGVRSLVGDLDAHT
                     SGGGIGLERIRGKMRVETSGGGIEGAELDGSIDAETSGGSVDLERVAGDIRAHSSGGG
                     IHIRDAGGRVEADTSGGGIEASFARGNARGGTLSTSGGGIRVAIDPSVGLRIDASGNS
                     VHADIPITVHGAISRGKLEGTLGGGGELLRLRTSGGGVRIQSIS"
     gene            complement(3580..4479)
                     /locus_tag="VF958_00520"
     CDS             complement(3580..4479)
                     /locus_tag="VF958_00520"
                     /inference="COORDINATES: protein motif:HMM:TIGR00765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HEX9147617.1"
                     /translation="MRKKLAAWRNVALTTFKNWFAHDSMAESAALAYYTVLSLAPVLL
                     VVVAIAGAVFGQDAVRGRLVDQFGGMMGKEQAALVQTILQKAERENTNGLAAVVGLVT
                     LIVGATAVFSQLQASLNRVWEVQPKRGHFLRDFIQKRLLSFAIVVAIGFLLLVSLVVS
                     TGIEALRNYVSQRFSTEPVWLEWANALLSFALFALLFAMIYRILPDARIPWRDVWLGA
                     IVTSLLFVFGKWAIGFYLGRTAATSAFGAAASVIVLLLWVYYASFIVLFGAEFTHAYA
                     KLLEEHVTPPEAGAKRQPRKRSA"
     gene            4551..>5511
                     /locus_tag="VF958_00525"
     CDS             4551..>5511
                     /locus_tag="VF958_00525"
                     /inference="COORDINATES: protein motif:HMM:NF013799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HEX9147618.1"
                     /translation="MITLNRRSLLRTFALISLAAVLRCGPPSAPRAAVPPPPTGGRAV
                     LISFDGTAGERLERMLDDSSKLPLGAFRRLSQRGFHAVRSHPPTPSLTAVSHIVHVTG
                     ALPQVTGIVSNSMLDRMKPFGSTISGFDAPIRAETLWEAARRQGRRVGVMLYPGADGN
                     GPARTADWMMTWPGNPLAPGRLHPIAASAWQPVSGSEAPGSFSPARKIVLGFEKTSHS
                     VAFIALDATDDGRMDYDRLLVLPESGGRGEVRVGEWFPVEVPNGDGRTGAWCKLLALA
                     PDLARAEIYIGPLSRSTGAPREWVRRLDEEIGFWPGMPDTDAFG"
BASE COUNT          863 a         1882 c         1879 g          887 t
ORIGIN      
        1 cgccgcacgt tggaaagcag aagtagcgtt ccttttcgtt tccgaagaag cggatcccct
       61 ccgcggcgag cttctcgagc gtctcgggct gcgcgccctc cgactcgagc agggttcgct
      121 tgttccgtgc cccgccgagc gagtagttcc cgatgtgccc gtcgcttcgc acgacgcggt
      181 ggcacgggat caggagcggc accggattct tcgcgagcgc ggatccagtc gcacgcacgg
      241 cgtcgggatg cccgatctcc cgcgcgatcc agctgtacgg ccgcacttcc ccgggtggaa
      301 tctcgagcgc cttccggagg acggcctgct cgaactcgga gagcccgcgc aggtcgaatc
      361 gcagtccggc gtcgcgggcg cggaggcgcc cttcggcgag ctcgcgcacg ttgcgcggcg
      421 gcgcggtcgc gtccggcgcg atcggccggc cgaatctctt ctcgaacgtc ttcgcgaact
      481 cggtggcgga gcgcgcccgt gtgaccatcg aaatgccggc ccggctgtgc gcgacgaaaa
      541 ccggtccgat cgacgtctcg agccgccagt agctgtcgcc gagaccgacc cggcgcagca
      601 cctcggggag aagcgacttc ggcgcacgct cccgcggaag gcccttcaat ccgcgcacga
      661 tcgcgatcgt tccgttgacg gaagcccgtt cgattctttt catgccctca cctctgcgcg
      721 cccttcgacg cggcgggaat ccgtgatcgc ccggcgcagc atgttgaccg cccggtggac
      781 gttcgacttc gccgttccga ggggttgttt gagaatgtcc gccacctctt cgagggagag
      841 cccctcgacg taccggagga tgatggcgga ccgatagcgt tccggaagcg tcgcgacaag
      901 cgaggcgatg tcggcgcgct gctgtccccg ctcgaagcgc gaatcgggtc gcgccatcgg
      961 gtcgtccggc gaatcccagg ccttctgcgc cgctgcaccg ttcgcaccgc cgttgtcgat
     1021 cgagacgtgc ggcggccgct tctttctcat gcggttgcgc gcgacgttca gcgtgatctg
     1081 gtagagccag gcctggaggg cgagcgcgcg gacgcgctcg ggcggatagc tcttgagcgc
     1141 ccgataggcc cggacgaaag cgtcctggac gacctcctgg gcgtcctccc ggctcgcaca
     1201 gatccgatgg gcgaacgcgt aaagacggtc ctggaacacg cggacgagac gctcgaacgt
     1261 cccgtcgagg tcttcggaaa gcgcgttcac cagctctctc tgcgccatga ctccctctcc
     1321 tcgctcgccc ggatacggtc gccgctcgac gagccgcaga cgcaatccgt cattaccggt
     1381 cccggagttc gtcgcacagg tcgtactcat cgcgccctct catcccgtaa aacacggggg
     1441 gcgggcgaaa ggttgcaggc gaaggcgcgg gctttcgtca ggccgggacg tatttccggc
     1501 gcctctcgat cgctcccgcg atccacacgg cgtggtgacg gctgttctcg atgaagatcc
     1561 ggccgatctc ggtgccgccg aggatcgcgc cggccagata gaggttggga acgttcgact
     1621 cgagggtctc cggatcgtgc gccggagccc tctccgggcc ctcgacccgg atgccgcagc
     1681 gctcgaggag cgcgaagtcc ggctcgtatc cgatcagggg gaacacggcc gtggcggcga
     1741 ccgactccgg tccggcgggc gtttcgacgc gaacgagctc gggcgtgatc tccacggccc
     1801 gcgcctcgaa gtgagctcgg atcgcgcccg cgctgatgcg gttttcgagg tccggcttga
     1861 tccaatactt cacgcgatcc gagatcgccg gcccccggac gatcatcgtg acgcgcgcgc
     1921 cgtggcgata gaggtcgagc gccgcctcgg aagcggagtt cttgccgccg atcacgacga
     1981 cgtcgcgccc gtgaaaggga tagccggaga cgaaacgccg gtgaacgtgg ggaaggtctt
     2041 cgccgggcac gccgagccgc cggggattgt cgaagaagcc gggcgcgaga atcccggcgg
     2101 gagccggaaa ccgtcgcggg ccaccggcgt cgaccgccga gacctcgaca ccgccggagg
     2161 gcacggccgt ggtcgcgacg atttccgtcc ggggcacgac gcgaactccg aatttctcgg
     2221 cgacggcgcg gtagtaagcc agagtctcga ggcgcgtcgg atgcgcgtcg ggaacgccga
     2281 aggggacacc cgcgatatcg aggaggtcgc gcgtggagaa ccaccgcatt tcctcgggga
     2341 agtggtagat cgagtgcagg aggtgcccgc ggtcgtagca gaccgccggg attcctcgat
     2401 tgccgagctc gatcgcgcag gcgatgccgg tcggcccggc tccgacgatc gcgacgggtt
     2461 ccacgccggg attctacgcg gcgcgccgtt tgccgcgggt acctatcagg ctcgtcggga
     2521 tccggtcttc cggagctacg agatgctctg gattctcact cctccgccgc tcgtgcgcag
     2581 acgcagcagc tcgccgcctc cgccgagcgt cccctccagt ttcccgcgcg agatcgcacc
     2641 gtgcaccgtg atcgggatgt cggcgtgcac ggagtttccg gaggcgtcga ttcggagccc
     2701 gaccgatgga tcgattgcca cccggatccc tccgcccgag gtcgagagcg tcccgccgcg
     2761 cgcgttgccc ctcgcgaagg aagcctcgat gccgccgccg gacgtgtccg cctccactcg
     2821 accgcccgcg tcgcggatgt ggatcccgcc ccccgagctg tgcgcgcgga tgtcacccgc
     2881 cacgcgctcg agatcgaccg agccgccgct cgtctccgcg tcgatcgagc catcgagctc
     2941 ggctccttcg attccgccgc cggacgtctc gacgcgcatc tttccgcgga tccgctcgag
     3001 gccgatgccg ccgccggacg tgtgcgcatc gaggtctccg acgaggctgc gaaccccgat
     3061 ccctccgccg gacgtgtgca gccttgcgtc cgactgcagc gaggcgatct tgatggctcc
     3121 gccggaggtg tcgatgtcca cccgggtgtg ctccggcacc tggacctccc agtggacgga
     3181 gtctccgccg gagttgaacc atccgaaaat ccgtccgtgt cggcgcctgg ccgtgaccga
     3241 gacggacccc gcgtcttcac ggaagttgaa gcgcaggagc tcgtcgagat cgcgccgctt
     3301 cgaggtgatg acgagccgcg cacccggcgc ggcggttccc gtgacggtga cgctccccat
     3361 gtcggtgtcg agcgaaaacc ggcctcccgg ttcgagccga agggccttct ccaggcgcgt
     3421 tccggcagaa agtgagagcg ccgggacgaa cagcccggcg agcaccacgg gcaggacggg
     3481 ggcgagacgg actctcatgg aaccctcctc cggcgcgaca gagttccgcc ggcctcttcc
     3541 ggctctaacg ctaccggggg gaaaaggttc cggtccggct caggccgatc tcttccgcgg
     3601 ctgccgtttg gctccggcct ccggcggcgt gacgtgctcc tcgaggagct tcgcgtaggc
     3661 gtgggtgaat tccgccccga agagaacgat gaacgatgcg tagtagaccc agagcaggag
     3721 aacgatgacc gaggcggcgg cgccgaaggc cgaggtcgcg gcggttctgc cgaggtagaa
     3781 gccgatggcc cactttccga agacgaacag gagcgaggtc acgatcgcgc cgagccagac
     3841 gtcgcgccac gggatccggg cgtccggaag gatgcgataa atcatcgcga agagaagcgc
     3901 gaagagcgcg aacgaaagca gagcgttggc ccattcgagc cataccggct cggttgagaa
     3961 tcgttgcgat acgtagttgc ggagcgcttc gattcccgtg gaaaccacga gcgatacgag
     4021 aaggagaaag ccgattgcga cgacgatcgc gaacgacagg agccgttttt gaatgaagtc
     4081 cctcaggaag tggccccgct tcggctggac ctcccacaca cggttcaggg aggcctggag
     4141 ctgcgagaaa accgcggtcg ctccgacgat cagggtgacg agcccgacca cggctgcgag
     4201 accgttcgtg ttttcccgct ccgccttctg gagaatcgtc tgaacgagag cggcctgctc
     4261 cttgcccatc atccctccga actggtcgac cagccggccc cgcacggcgt cctgcccgaa
     4321 gacggcgcct gcgatcgcga cgacgacgag caggacgggg gcgagcgata agacggtgta
     4381 ataggcgagc gccgcgctct ccgccatcga gtcgtgcgcg aaccagttct tgaacgtcgt
     4441 gagggcgacg ttccgccacg cggcgagctt tttcctcatc ccacggatgt ccggggcaaa
     4501 cggcgtgccc acctcgcgtc gcaagcggcc tgttacgatc ctcgcaccgc gtgatcacgt
     4561 tgaatcgacg atcgcttctc cggaccttcg cgctgatctc gctcgcggcg gttctccgct
     4621 gcggtccccc ctccgccccg cgcgcggccg ttccgccgcc tccgacgggc gggcgagcgg
     4681 tgttgatctc gttcgacgga accgccggcg aacgcctgga gcgcatgctc gacgattcct
     4741 cgaagctccc cctgggagcg ttccgccgcc tctcccagcg tggatttcac gcggtccggt
     4801 cgcacccgcc cacgccgtcg ctgacggccg tgtcgcacat cgtgcacgtc accggggcgc
     4861 tgcctcaggt aacggggatc gtttcgaact cgatgctcga tcggatgaaa ccgttcggat
     4921 cgacgatctc gggcttcgac gcgccgatcc gcgccgaaac gctgtgggag gcggcgcgga
     4981 ggcagggcag gcgggtgggc gtgatgctct acccgggcgc cgacggaaac gggcccgcgc
     5041 ggacggccga ctggatgatg acgtggcccg gcaaccccct cgcccccggc cgcctgcacc
     5101 cgatcgccgc gtccgcctgg cagcccgttt ccggctccga ggccccgggg agtttctcgc
     5161 cggcccggaa gatcgtgctc gggttcgaga agacgtcgca ctccgtcgcg ttcattgccc
     5221 tcgacgcgac cgacgacgga cgcatggact acgaccggct gctcgtgctt ccggaatccg
     5281 gagggcgcgg tgaggtgcgg gtgggagagt ggtttccggt cgaagtcccg aacggggacg
     5341 gtcgcaccgg cgcgtggtgc aagctgctgg cgctcgcacc cgacctcgcg cgcgcggaga
     5401 tttacatcgg gcctctctcc cgctctacgg gagcgccgcg cgagtgggtg cgccggctcg
     5461 acgaagagat cggcttctgg cccggcatgc cggacacgga tgcgttcggc g
//
LOCUS       DASWAV010000026         2905 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U2665
            k141_641538, whole genome shotgun sequence.
ACCESSION   DASWAV010000026 DASWAV010000000
VERSION     DASWAV010000026.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960316
            Sequence Read Archive: SRR10260007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2905)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2905)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:47:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,929
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2905
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_641538"
                     /isolate="SMAG_U2665"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.36444 N 132.31889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..577
                     /locus_tag="VF958_00530"
     CDS             <1..577
                     /locus_tag="VF958_00530"
                     /inference="COORDINATES: protein motif:HMM:NF014385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HEX9147619.1"
                     /translation="LRDSEGNIISRESGGFGDSFQQILDRDFKTWSIGLNFSYPILNR
                     RARGQRGAALYTWESSKALLNSVEQNVLLEVRAAARDIDTARRTIAAAQKSRELAERN
                     LDAERKKFENGMTTSFQVTQIQNDLSAARSLELQALAVYRKALSAYHLAIADILDWKG
                     IRIEDLPQTEPPRPESRAPRVLAPAPPPAGQ"
     gene            574..1308
                     /locus_tag="VF958_00535"
     CDS             574..1308
                     /locus_tag="VF958_00535"
                     /inference="COORDINATES: protein motif:HMM:NF016764.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YIP1 family protein"
                     /protein_id="HEX9147620.1"
                     /translation="MSDTPENLEVADPAASPLARVAGVFGSPGPTFESIARRPTWLLP
                     VIISTVLSIAATAAIVPRMDLDSAVREAFAKRGISASEERIDRTIEVQKKFAGFFAYV
                     WALFAGALVALVLATVFWISFKAFGWDLAFRQSFGVTTHALLPYIGATMLLILFVTRL
                     DLVNPADLGDVSHSNLGFLVDRHANPVLNSIARSIDVFTLWVLALLVIGFASAAKVPR
                     KRAAAVIVGLWAVFVLGKAGWTAIFH"
     gene            1382..2116
                     /locus_tag="VF958_00540"
     CDS             1382..2116
                     /locus_tag="VF958_00540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014099065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX9147621.1"
                     /translation="MLIDIRDITKVYEMGAQKVRALDGVSLQVDRGEYVAIMGPSGSG
                     KSTLMNLIGCLDTPTSGSYVLNGREVARMTDDELAAIRNQEIGFVFQTFNLLPRTTAL
                     QQVELPLVYSGLPRKDRRDRAIKALEAVGLGDRMTHHPSELSGGQRQRVAVARALIND
                     PSILLADEPTGNLDSATGAEIMALFDVLNGKGNTIVLVTHEEDIAAHARRIVRLLDGR
                     VRDDGPNDRAAALARFGRAPVSSSPA"
     gene            2202..>2905
                     /locus_tag="VF958_00545"
     CDS             2202..>2905
                     /locus_tag="VF958_00545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9147622.1"
                     /translation="MESRKSLFRKVERAIDAIERSSDASSTIAETASAVIENFKDELG
                     VTGGRLYERRDGGYELTRVFGGAVAAPGIFVPDAYPPVQQVVDEGVVLMDLTAPGVDP
                     EFERKLGVDRFAAIAVGDDEYILSFSVDPVAPDDDLMASLGILRHAVDQKLREERFLS
                     VLIEARQIQMSILPKRSPRRGEFEIAGFTVPAEIVGGDFFDFIPVTDRIQGIAIADAS
                     GHGLPAALQVRDVYTGL"
BASE COUNT          463 a         1005 c          943 g          494 t
ORIGIN      
        1 gctgcgcgac tcggaaggca acatcatctc gcgcgagtcg ggcggcttcg gggactcgtt
       61 ccagcagatc ctggatcgcg atttcaagac ctggtcgatc ggcctgaact tctcgtaccc
      121 gatcctgaac cggcgggccc gcggccagcg cggcgccgcc ctctacacgt gggagtcgtc
      181 gaaggcgctc ctcaattcgg tcgagcagaa cgtgctcctc gaggtccgcg ccgcggcccg
      241 ggacatcgac accgcccggc gcacgatcgc ggcagcgcag aagtcccgcg agctcgccga
      301 gcgcaacctc gacgccgagc gcaagaagtt cgagaacggc atgacgactt cgttccaggt
      361 gacgcagatc cagaacgacc tctcggcggc tcgcagcctc gagctgcagg cccttgccgt
      421 ttaccgcaag gccctctccg cctatcacct cgcgatcgcg gacatcctcg actggaaggg
      481 catccgcatc gaggacctgc cgcagaccga gcccccgcga cccgagagcc gcgccccgcg
      541 ggtcctcgcg ccggcgccgc cccccgccgg ccagtgagcg atacgcccga gaacctcgaa
      601 gtcgccgacc ccgcggcttc tccgctcgcg cgagtggccg gagtcttcgg ttcgcccggt
      661 ccgaccttcg aatcgatcgc gcgccggccg acctggctct tgccggtcat catttcgacg
      721 gtcctctcga tcgccgcgac cgcggccatc gttccccgca tggatctcga ctccgccgtg
      781 cgcgaagcct ttgcaaagcg gggcatcagc gccagcgaag agcggatcga ccggacgatc
      841 gaagtgcaga agaagttcgc gggcttcttc gcctacgtgt gggcgctctt cgcgggcgct
      901 ctcgtcgcgc tcgtgctcgc gacggtcttc tggatctcgt tcaaggcttt cgggtgggac
      961 ctcgcgttcc ggcagtcgtt cggagtgacg acccacgcgc tccttccgta catcggcgcc
     1021 acgatgctcc tcatcctctt cgtcacccgc ctcgatctcg tcaacccggc ggacctcggc
     1081 gacgtcagcc actcgaacct cgggtttctc gtggatcggc atgcgaaccc ggtcctgaac
     1141 tcgatcgcga gatcgatcga cgtctttacg ctctgggtcc tggcccttct cgtcatcggg
     1201 ttcgcgagcg cggcgaaggt gccccggaag agggcggcgg ccgtcatcgt gggcctgtgg
     1261 gccgtcttcg tgctcgggaa ggccggatgg accgcgatct tccattgagg aatcggctcc
     1321 ggcaactcct cgggggaaaa atcgtatacg gcagaggggc gagcgccagc caggacacga
     1381 catgctgatc gacattcggg acatcacgaa ggtctacgag atgggcgccc agaaggtgcg
     1441 cgcgctcgac ggcgtctctc tgcaggtcga tcgcggcgag tacgtcgcga tcatgggacc
     1501 ttcgggatcc ggcaagtcga ccctgatgaa cctgatcgga tgtctcgata cgccgacgtc
     1561 cggctcgtac gtgttgaacg ggcgcgaagt ggcgcggatg acggacgacg agctcgccgc
     1621 gatccgcaac caggagatcg ggttcgtctt ccagacgttc aacctgctcc ctcgaacgac
     1681 ggcgctccag caggtggagc tgccgctcgt ctactcgggc cttccgcgga aggatcgccg
     1741 cgaccgcgcg atcaaggcgc tcgaagccgt gggcctgggc gaccggatga cgcaccaccc
     1801 gagcgagctc tccggcgggc agcgccagcg cgtagccgtc gcccgggcgc tcatcaacga
     1861 tccttcgatc ctgctcgccg acgagcccac cgggaacctc gactccgcga cgggcgccga
     1921 aatcatggcc ctcttcgacg tgctgaacgg caagggaaac acgatcgtgc tcgtcacgca
     1981 cgaagaagac atcgccgcgc acgcgcgccg catcgttcgc ctcctcgacg gccgggtccg
     2041 ggatgacggc ccgaatgatc gcgctgcggc gctcgcccgt ttcggcagag cgccggtctc
     2101 ttcttctccc gcttaggtcg tctctttcgc atcgcgaaga gtctccgcga agtaaccgag
     2161 cggaggagct tcgcgacggg atagaatcga gcgttttcgc catggagtcc cgcaagtctc
     2221 tcttccgcaa ggtcgaacgc gcgatcgacg cgatcgagcg atcgtcggac gcttcgtcga
     2281 cgatcgccga gaccgcgagc gcggtgatcg agaatttcaa ggacgagctc ggcgtgacgg
     2341 ggggacgcct ctacgagcgg cgggacggcg gctacgagct gacgcgggtc ttcgggggcg
     2401 ccgtcgccgc gcccggaatc ttcgttcccg acgcgtaccc gccggtccag caggtcgtcg
     2461 acgagggcgt cgtgctcatg gacctgaccg cccccggggt ggatccggag ttcgagcgaa
     2521 agctcggtgt cgatcggttc gctgcgatcg ccgtcggcga cgacgagtac atcctctcgt
     2581 tctcggtcga tcccgtggcg cccgacgacg acctgatggc ttcgctcgga atcctgcgcc
     2641 atgccgtgga ccagaagctg cgcgaggagc gttttctctc cgtcctcatc gaggcgcgcc
     2701 agatccagat gtcgatcctg ccgaagcggt cgccccgccg cggagagttc gagatcgccg
     2761 ggttcacggt tccggccgag atcgtcgggg gggacttctt cgacttcatc ccggtcaccg
     2821 atcggatcca ggggatcgcc atcgccgacg cctccggaca cgggctgccg gcggcgctcc
     2881 aggtgcgcga cgtctacacg ggtct
//
LOCUS       DASWAV010000027         8165 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U2665
            k141_441650, whole genome shotgun sequence.
ACCESSION   DASWAV010000027 DASWAV010000000
VERSION     DASWAV010000027.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960316
            Sequence Read Archive: SRR10260007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 8165)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8165)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:47:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,929
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8165
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_441650"
                     /isolate="SMAG_U2665"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.36444 N 132.31889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..4807)
                     /locus_tag="VF958_00550"
     CDS             complement(<1..4807)
                     /locus_tag="VF958_00550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013952953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucoamylase family protein"
                     /protein_id="HEX9147623.1"
                     /translation="MQSDSAGSVLLSATGLEAPIRSELFSTERLEEFGERLGEEHRSA
                     RGFRRGRGHLPRLRENARVLLESYHSVADAIREERTISPAAEWLVDNFHIVEEQLREI
                     REDLPTPYYRELPKLEAGSLAGFPRVYALTYEYVAHTDSRFETEALRRFVLAYQHAAP
                     LTIGELWAVPIFFRIVLVDNLRRLAERIVQRRAAREQADALSDSLLGLVPSGASSWEE
                     QLHELDRRPLSIPFAVHLLQRLRERDPEQTPALAWLTRRIEEQGLTPDEIVRLEQQEQ
                     VATHITVRNVITSMRLVSAVDWAEFFESVSLVEKALRQGTHVEDMDFSTRDRYRHAVE
                     DLSRRSALSEIAVAELATRKAREAAAQGGSTAADARRADPGYYLISGGRREFEREVGY
                     RVPLTHYLKRTYFAAATPGYVGTILLLTAVVLAVPVALSVTAGEARWELLALALLALL
                     PASDVAVFIVNRDVTELVGPRRLPKLALEQGIPRELHTLVVVPSLLTSEAAVRELAER
                     LEVHYLANADDELRFALLTDWEDAPEESADDDEALFALAAEAIDDLNRRHGNLPDGSR
                     RFLFLHRRRVRNEKEKKWMGWERKRGKLHELNLLLRGAAATTFFPVEPLDRAALSRVR
                     YVLTLDADTRLPRGAAKKLVGTMAHPLNRPVYDAKVGRIVDGHGVLQPRVTPTLPFSG
                     PGTLYQRLHSGPAGIDPYAAAVSDVYQDLFGEGIYTGKGIYHVDEFERSLAGRIPDEA
                     LLSHDLFEGLFSRAGLVTDVELFENFPPHYGLAAARMHRWARGDWQLLPWLGFRVRDA
                     SGRRVRNRISLIGEWKIVDNLRRSLSAPASFLVLVASWVLPGAPAAVWTLAVLAAFLA
                     PGLFSVLAGIIPGRRGISKRSFLRGIGHDLAVELGRSVFSLVVLAHQAWLMGDAIART
                     LFRLFVSKRRLLEWVTAAQAAGELDLGLAGFYRRMWGALVLASGAGIAAATAGKGALS
                     IALPFVVAWFASPWVAWRASLPPRQTESQGLSDEERRTLTLTARRTWRFFETFVTAEE
                     NHLPPDNFQEDPRPTIAHRTSPTNISLYLLSTVSARDFAWIGTIDMVERLEAAMATIQ
                     KLEKYRGHLYNWYETRRLDPLEPRYVSTVDSGNLAGHAIVLERACINQLDAPAVHPRA
                     LAGIGDALALAREALAATAPGTRGGSITRRDVEGAMRDVEELLAAPPENVSQWGARLA
                     ALRERAEILADSTEALASELADEPSSEMHYWSKATLAAIASHERDFGAFVSWMRESPD
                     SGIPILRALPDLCETAQTRSQTGGGSAAPSPPELERCREASTAIAGRLSVLARTAASL
                     VAAMDFRFLYDEERKLFTIGFRVTDGRFDPGYYDLLASETRLASFIAIARGDTPPSHW
                     FHLSRPLTPVGTGSALVSWSGSMFEYLMPDLVMNEPTGSVLEVTDRLVVARQIEYGGE
                     RRAPWGVSESAFYARDLDLTYQYSNFGVAGLGLKRGLTEDYVVAPYATALASMVDPAA
                     AAHNFERLAKEGGLGRYGYYEALDYTASRIPEGKTHAIVRAYMAHHQGMTVVALANTL
                     LGEVMRKRFHEEPRVQATELLLQERMPRGVAVARPRAEEVQTHLHVRDYV"
     gene            complement(4899..5261)
                     /locus_tag="VF958_00555"
     CDS             complement(4899..5261)
                     /locus_tag="VF958_00555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9147624.1"
                     /translation="MTRSKSAIGRKTVSRRASGKRGTARRRSGKPALLEGLLNDLAKR
                     ANRAGETIASLSEEGATAARKTLGAVTARSKKTIARVRREWDGMDNTRRAQFVGALLG
                     ALAAAGAAAASVRKATKR"
     gene            complement(5423..7153)
                     /locus_tag="VF958_00560"
     CDS             complement(5423..7153)
                     /locus_tag="VF958_00560"
                     /inference="COORDINATES: protein motif:HMM:NF015530.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HEX9147625.1"
                     /translation="MRISKTGLFFSAALAALAAGCAREKAAAPAGQPGGQVSSPAASS
                     GLAFAPDIPDRLRKLARTPIDYDHALLDAKETQVVAKLIEASRYLNNIFLNQVWEGNP
                     ALRGQIAAAAGKGDSTARWALALFDVHKGPWDRLKKNEPFVGKDPKPTGAGFYPTDMT
                     KEEFEKWVAAHPQDKAAFEGLFTVIRRQGQSLVAIPYSKAYGPILQPAAQALRQAAAL
                     TGNASLRDYLNKRADAFLSDDYYASDIAWMDLDSDIEVVIGPYEVYEDALFNYKAAFE
                     SFVTVRDRTESEKLAVYAKHLPDMERNLPIPEAHKNLRRKFESPIRVVQEIYTAADAR
                     SGVQTSAFNLPNDERVREAKGSKKVLLKNVMDAKFQQSGRPIAERILVAGELPNLSFD
                     AYFDHTLFHELSHGIGPGLITGPDGKRVDSRLLLKEIYSTLEECKADVTGLWNILLAL
                     DRGWLTGISEAQLFSTYNGLMFRGMRFGIDEAHGQGTAIQWNWFREKGAISPAPEGRF
                     QTDVPKFREAVRSLANELLEIEATGDYARGKALLGKYGRSTPEVEATIAKLSDIPVDI
                     APVFVAAGER"
     gene            7203..>8165
                     /locus_tag="VF958_00565"
     CDS             7203..>8165
                     /locus_tag="VF958_00565"
                     /inference="COORDINATES: protein motif:HMM:NF024273.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HEX9147626.1"
                     /translation="MPQVEAPTDRFLDRRRAVDWYRSNRRRSAEIFDLLSPDAYYDRP
                     IALRNPIAFYEGHLPAFSVNTLLKKGLGERGIDAGFEVLFERGIDPEDVSSVPGVAPR
                     GWPSRSEIQAYGRDADRLIFEALESKDVTRADNPVLRRGLAAYTILEHEEMHQETLLY
                     MWQRLPYEKKHAPRGAPSPLLGGEPPRERRRVRVPAGRATLGARRDRILFGWDNEFDE
                     VAVEVSDFEIDVHDVTNRQFLEFIAAGGYDRDDLWSAEGWEWRVRNDVRHPVFWERRG
                     GEWVWRGMFGDLPLPPSWPVFVSHAEASAYAKWRGARLPTEAEFH"
BASE COUNT         1283 a         2706 c         2847 g         1329 t
ORIGIN      
        1 ggacgtagtc gcgcacgtgg aggtgggtct ggacctcctc ggcccgcgga cgcgcgaccg
       61 ccactccgcg cggcatgcgc tcctgcagga gaagctcggt cgcctgcacc ctcggctctt
      121 cgtggaaacg tttccgcatg acctccccga gaagagtgtt cgcgagggcg acgaccgtca
      181 tgccctggtg atgcgccatg taggcgcgca cgatggcgtg ggtcttgccc tcaggaatcc
      241 ggctggcggt gtagtcgagc gcctcgtagt agccgtagcg gccgaggccc ccctctttcg
      301 cgagccgctc gaagttgtgc gcggctgccg ccggatcgac catcgaagcg agcgccgttg
      361 cgtagggcgc cacgacgtag tcctcggtca ggccacgctt caagccgagc cccgcgacgc
      421 cgaagttcga gtactggtac gtgagatcca ggtcgcgcgc gtagaacgcc gactcggaaa
      481 ccccccacgg cgcgcgccgc tctccgccgt actcgatctg tctcgcgacc acgagccggt
      541 cggtgacttc caggacgctt ccggtcggct cgttcattac gaggtctggc atcaggtact
      601 cgaacatcga gccggaccag gagacgagcg ccgatcccgt tccgacgggc gtcagcgggc
      661 gcgacaggtg gaaccagtga gacggcggcg tgtcgccgcg cgcgatcgca atgaagctcg
      721 cgaggcgcgt ttcggaggcg agaaggtcgt aatagccggg atcgaaccgg ccgtcggtca
      781 cccggaaacc gatcgtgaag agcttgcgct cctcgtcgta gaggaatcgg aagtccatcg
      841 cggcgacgag agacgctgcg gtcctggcga ggacgctcaa gcggcccgcg atcgccgtcg
      901 aggcctcccg gcaccgttcg agctcgggcg gagacggtgc ggcggagccg ccgccggtct
      961 gcgaccgggt ttgcgctgtt tcgcacagat cgggaagcgc ccggagaatg gggatcccgc
     1021 tgtccgggct ctcccgcatc cacgaaacga acgcgccgaa gtcgcgctcg tggctcgcga
     1081 tggcggccag agtagctttc gaccagtagt gcatctcgct cgacggctcg tcggccagct
     1141 cgctcgcgag ggcctcagtg gaatctgcga ggatttccgc gcgttcgcgc agggcggcga
     1201 gtcgcgcccc ccactgcgac acattctcgg gcggggccgc caggagctcc tcgacgtcgc
     1261 gcatcgcgcc ctcgacatcc cggcgtgtga tcgagcctcc gcgtgtgccc ggcgccgtcg
     1321 cggcgagcgc ctcgcgggcg agagcgagag catccccgat ccccgcgagc gcccgcggat
     1381 ggactgccgg cgcgtcgagc tggttgatgc aggcgcgctc gaggacgatg gcatgtcccg
     1441 cgagatttcc gctgtcgacg gtggaaacgt agcgtggctc gagcgggtcg agacggcgag
     1501 tctcgtacca gttgtagagg tggccacggt acttctcgag cttctggatc gtcgccatgg
     1561 ccgcctcgag ccgctcgacc atgtcgatcg tcccgatcca cgcgaagtcg cgagcggaga
     1621 ccgtcgaaag cagatagagg ctgatgttcg tcggggacgt ccggtgagcg atcgtcggac
     1681 ggggatcctc ctggaaattg tcaggcggta ggtggttctc ctcggcagtc acgaacgtct
     1741 cgaagaaccg ccacgtccgc cgcgccgtca gcgtgagagt ccggcgctcc tcgtcggaca
     1801 atccttgcga ctccgtctgc cgagggggaa ggctcgcgcg ccacgccacc cagggggacg
     1861 cgaaccacgc gacgacaaag ggcagcgcga tcgagagcgc tcccttgccg gcggtcgcgg
     1921 cggcgatccc tgcgcccgag gcgagcacga gggcgcccca catgcgccga taaaagcccg
     1981 cgagcccgag gtcgagctcg cccgccgcct gcgccgccgt cacccactcg agcagacgcc
     2041 gcttcgagac gaagagccgg aaaagcgtgc gtgcgatggc gtcgcccatg agccacgcct
     2101 ggtgggcgag gacgacgagc gagaacacgc tgcgtccgag ctcgacggcg aggtcgtgcc
     2161 cgatcccgcg caggaagctc ctcttcgaga tccctcgccg gccggggatg attccagcga
     2221 gcacggagaa gagaccgggc gcgaggaacg ccgcgagaac ggcgagcgtc cacacggccg
     2281 ccggcgcgcc ggggagcacc caggacgcga cgagcacgag gaacgaagcg ggagcagaga
     2341 gcgagcgccg caggttgtcg acgatcttcc actccccgat gagcgaaatc cggttgcgca
     2401 cccgccgccc ggaggcatcc cggacccgga agccgagcca cggcaggagc tgccagtcgc
     2461 cgcgcgccca ccggtgcatc cgcgcggcgg cgagcccgta gtgcggaggg aagttctcga
     2521 agagctcgac gtcggtcacg agacccgctc gagagaaaag gccttcgaag aggtcgtgcg
     2581 agagcagggc ctcgtcgggt atccggccgg cgagcgacct ctcgaactcg tcgacgtggt
     2641 agatcccctt gcccgtgtag atcccttccc cgaagaggtc ctggtagacg tcggagaccg
     2701 ccgcggcgta gggatcgatg ccggccggcc ccgagtggag ccgctgatag agagtcccgg
     2761 gccccgagaa cggaagcgtg ggcgtgaccc gcggctggag cacgccgtgt ccgtcgacga
     2821 tgcggccgac cttcgcgtcg tagaccggac ggttcaatgg gtgcgccatc gttcccacga
     2881 gcttcttcgc ggcgccgcgg ggaagcctcg tgtcggcgtc gagcgtcagc acgtagcgca
     2941 cgcgggacag ggcagcgcgg tcgagaggct cgacgggaaa gaacgtggtt gcggccgctc
     3001 cgcggagcag gaggttgagc tcgtggagct tgccgcgctt gcgctcccag cccatccact
     3061 tcttttcctt ctcgttccgg acccggcgac gatggaggaa aaggaagcgc cggcttccgt
     3121 cgggaagatt cccgtggcga cggttgaggt cgtcgatcgc ttccgccgcc agtgcgaaca
     3181 gcgcttcgtc gtcatcggcc gactcctcgg gggcgtcctc ccagtccgtg aggagggcga
     3241 agcgcagctc gtcgtccgcg ttggccagat agtgcacttc gagacgttcg gcgagctcgc
     3301 gcacggcggc ctcgctcgtg aggagcgacg gcacgacgac gagcgtgtga agctcccgcg
     3361 ggattccctg ctcgagagcc agcttcggga gccgccgcgg tccgacgagt tccgtgacgt
     3421 cgcggttgac gatgaagacg gccacgtcgg acgccgggag gagggcgagg agagcgagcg
     3481 cgaggagctc ccaccgcgcc tcgcccgccg tcaccgacag ggcgacgggc accgcgagca
     3541 cgaccgctgt gaggagaagg atcgtgccga cgtatcccgg cgttgcggcg gcgaaatacg
     3601 tgcgcttcag gtaatgagtg agcgggacgc gatagccgac ctcccgctcg aactcccgcc
     3661 gcccgcccga gatcaggtag tagccggggt ccgcccggcg cgcgtccgca gcggtcgatc
     3721 caccctgtgc ggcggcctcg cgggctttcc tggtcgcgag ctcggcgacc gcgatctcgg
     3781 agagcgcgct gcggcgggag aggtcttcca ccgcatggcg gtacctgtcg cgggtcgaga
     3841 aatccatgtc ctcgacgtgc gtcccctgcc ggagcgcctt ctcgacgagg ctgaccgatt
     3901 cgaagaattc cgcccagtcg accgccgaca cgagccgcat gctcgtgatc acgttccgaa
     3961 cggtgatgtg cgtcgcgacc tgttcctgct gctcgagccg cacgatctcg tccggggtca
     4021 agccctgctc ttcgatgcgt cgcgtcagcc aggcgagggc gggcgtctgt tcgggatccc
     4081 gctcgcggag ccgctggagg aggtggaccg cgaaggggat cgagagcggg cgccggtcaa
     4141 gctcgtggag ctgctcctcc cacgaagagg cacccgacgg gacgagtccg agcagggagt
     4201 cgctcaaagc gtcggcctgc tcccgggcag cccgccgctg gacgattcgc tcggcgagtc
     4261 ggcgcaggtt gtccacgaga acgattcgga aaaagatcgg gaccgcccag agctcgccga
     4321 tcgtcagcgg agccgcgtgc tggtaggcaa gaacgaagcg acggagggcc tccgtctcga
     4381 atcggctgtc ggtgtgggcg acgtactcgt acgtcagggc gtagacgcgc gggaagccgg
     4441 ccagcgatcc cgcctcgagc ttcgggagct cccggtagta gggggtgggc aggtcctcac
     4501 ggatctctcg aagctgctcc tcgacgatgt gaaagttgtc gacgagccat tcggcggcgg
     4561 gcgagatcgt ccgctcctct cggattgcat ccgccaccga gtgataggac tcgaggagga
     4621 cccgcgcgtt ctcgcgcagg cgggggaggt ggccccggcc gcgccggaat ccacgggcgc
     4681 ttcgatgctc ttcgcccagc cgttccccga actcttccag gcgctcggtc gaaaagagtt
     4741 cggatcgaat cggtgcttcg agaccggtgg cggaaaggag gacggacccg gcgctatctg
     4801 actgcatctt cttcaacggc ggcgaggcgg gcgggggccc ctcgccgggg ggacgatcgt
     4861 cacgcgggcg gcgaggctcg ccgcgctcgg tcgggccgct acctcttcgt cgctttccgg
     4921 acgctggccg ccgcggcgcc cgccgccgcg agagcgccga gcaacgcgcc gacgaactgc
     4981 gccctccgcg tgttgtccat cccgtcccac tcgcgccgga cgcgggcgat cgtctttttc
     5041 gatctggcgg tgaccgcccc gagcgtcttt cttgcagcgg tcgcaccctc ctcggacagg
     5101 ctggcgatgg tctcgcccgc gcggttggct ctcttcgcga ggtcgttcag gagcccttcg
     5161 agaagggctg gtttccccga gcgccgccgc gccgttccgc gcttgccgga cgcccggcgg
     5221 gaaaccgtct tccgtccgat ggccgacttc gaccgtgtca cggcctttcg ccggcgcgtt
     5281 cccccggttc tcttgcgggt cgtgccggag gagcccgacg atccgctgga cgtcgaggaa
     5341 gcgctctcag ctccttcgtt ttccgtcact ctgacctcct tgttccggtc tttctcagca
     5401 agattgagtc cagtttccgc cgtcaccgct cgccggcggc gacgaagacg ggggcgatgt
     5461 cgacgggaat gtccgaaagc ttcgcgatcg tcgcttccac ctccggcgtc gaacggccgt
     5521 acttcccgag aagagccttt ccgcgcgcgt agtcgccggt cgcttcgatc tcgaggagct
     5581 cgttcgcgag cgaccgcacc gcctcgcgaa acttcggcac atccgtctgg aaccgccctt
     5641 ccggcgccgg gctgatcgcg cccttttctc ggaaccagtt ccactggatc gcggtcccct
     5701 ggccgtgcgc ctcgtcgatc ccgaaccgca tgcctcggaa catgagcccg ttgtaagtcg
     5761 agaagagttg cgcctcgctg atcccggtga gccagccgcg atcgagcgca aggaggatgt
     5821 tccagagtcc ggtgacgtcg gccttgcact cctccagagt cgagtagatc tccttcagga
     5881 ggagtcgcga gtcgacgcgc ttgccgtccg gacccgtgat gaggccgggg ccgatcccgt
     5941 gcgagagctc atggaacagc gtgtggtcga agtatgcgtc gaaggagaga ttcggcagct
     6001 ccccggccac gaggatccgc tcggcgatcg gtcggccgct ctgctggaac ttcgcgtcca
     6061 tcacgttctt caggaggact ttcttcgagc ccttcgcctc gcggaccctc tcgtcgttgg
     6121 gcaggttgaa tgcggacgtc tggacgccgc tgcgcgcgtc ggcggccgtg tagatctcct
     6181 ggacgacgcg aatcggcgat tcgaatttcc tccggaggtt cttgtgcgct tcggggatcg
     6241 gcaggttgcg ctccatgtcc ggcaggtgct tcgcgtacac cgcgagcttc tcgctctccg
     6301 tccggtcgcg gaccgtcacg aaagactcga aagcggcctt gtagttgaag agcgcgtcct
     6361 cgtaaacctc gtagggcccg atgacgactt cgatgtcgga gtcgaggtcc atccaggcga
     6421 tgtcggaggc gtaatagtcg tcggagagga acgcgtccgc gcgcttgttg aggtaatcgc
     6481 gcaggctcgc gttgccggtc agggccgcgg cctggcgcag ggcctgcgcg gcaggctgga
     6541 gaatcgggcc gtacgccttc gagtaaggga tcgccacgag cgattgcccc tgtcgtcgga
     6601 tgaccgtgaa gagcccttcg aaggccgcct tgtcctgcgg gtgggcggcg acccacttct
     6661 cgaactcctc tttcgtcatg tccgtcggat agaaccccgc gcccgtcggc ttcggatcct
     6721 ttccgacgaa cggctcgttc tttttcagcc ggtcccacgg gcccttgtgc acgtcgaaga
     6781 gggcgagcgc ccagcgcgcc gtggagtcgc ccttcccggc cgcggcggcg atctgaccgc
     6841 gaagagccgg atttccttcc cagacctggt tcaggaaaat gttgttcagg taccgggacg
     6901 cctcgatcag tttcgcgacg acctgcgtct ccttcgcgtc gagcagagcg tggtcgtagt
     6961 cgatcggagt gcgcgcgagc tttcggagcc ggtccgggat gtccggggcg aaggcaaggc
     7021 ccgaggaagc cgcgggcgag gagacctgtc cccccggctg accggccggc gcggcggctt
     7081 tctcccgcgc gcagccggcg gcgagcgcgg ccagggccgc agaaaagaag agaccagtct
     7141 tcgagatgcg cattggcggg aatctagcat tttcgaggac gcgaatgggt aaagtaagag
     7201 ccatgccaca ggtggaggct ccgaccgacc gattcctcga tcgccgtcgc gccgtcgact
     7261 ggtaccgctc gaaccgccgc cggtcggcgg aaatcttcga cctgctctcg cccgacgcct
     7321 actacgaccg gcccattgcg ctgcggaatc cgatcgcgtt ctacgaaggg catctgccgg
     7381 ccttctccgt caatacgctc ctgaaaaagg gactcgggga acgcggcatc gatgccggct
     7441 tcgaggtcct cttcgagcga ggcatcgatc cggaagacgt ctcatcggtc ccgggcgtcg
     7501 cgccgcgcgg gtggccttcg cgctccgaga tccaggcgta tggacgagac gcggaccggc
     7561 tcatcttcga agccctcgag tcgaaggacg tcacccgcgc ggacaacccc gtcctgcgac
     7621 ggggactcgc ggcctacacg atcctcgagc acgaggagat gcaccaggag acgcttctct
     7681 acatgtggca gcggctgccg tacgagaaga agcacgcgcc gcgcggcgcg ccgtcgcccc
     7741 ttctcggcgg ggagcctccg cgggagcggc ggcgggtgag agttcccgcc ggccgagcga
     7801 ctctcggggc gaggcgggat cgcatcctct tcggctggga caacgagttc gatgaagtcg
     7861 cggtcgaggt ctccgatttc gagatcgacg tgcacgacgt gacgaaccgc caattcctgg
     7921 agttcatcgc agcgggcggg tacgaccggg acgacctgtg gtcggcggag ggctgggagt
     7981 ggcgcgtgcg caacgacgtt cgccatcccg tgttctggga gcggcgcggc ggcgagtggg
     8041 tctggcgggg gatgttcggc gacctcccgc tccctccctc gtggcctgtc ttcgtcagtc
     8101 acgcggaggc gtccgcgtac gcgaaatggc gcggcgcccg gctcccgacc gaagcggagt
     8161 tccac
//
LOCUS       DASWAV010000028         2986 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U2665
            k141_241379, whole genome shotgun sequence.
ACCESSION   DASWAV010000028 DASWAV010000000
VERSION     DASWAV010000028.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960316
            Sequence Read Archive: SRR10260007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2986)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2986)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:47:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,929
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2986
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_241379"
                     /isolate="SMAG_U2665"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.36444 N 132.31889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(266..>2986)
                     /locus_tag="VF958_00570"
     CDS             complement(266..>2986)
                     /locus_tag="VF958_00570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9147627.1"
                     /translation="TSYAESAYDEISRALNFQIPKPINLIYYASHSDFEQTNTILNFI
                     PEGIGAFALPSRNRMVLPIDLPDETLQQLIAHELTHVFQFEIIFGGNYIRAATTNAPQ
                     WFMEGMASYFGNDEDNKDRMVLRDAVLADQVPEIAQTGAISGFFAYRFGHSVFDFIEA
                     EWGKDSVRDFVFEFRNQIGGNVEKVIKRAFNISAEDFDVKFRRYLRQRYLKILTEKGE
                     PIDFGERFKLVEEPSAELTPRAYPSGDFVAAISTLKNDADVVVLSTQDRKLYRNLTRG
                     YTTRYEYIVSQWLTTGPLGGGDIAVAPDGNTVGVFARRERGRELVLLNALDGGIRERI
                     EMPGLDQQLNPAFSPDGNTVVFRALKGGHADIWAYSLTSKTLTNLTDDAAFDFAPTYS
                     PDGKWIYYSSVRGTKAKIFRFDPTSPGSRQQITYGDWNDEDAALTPDSKRLFFTSDRD
                     GGIFNIYSINLETGETNLHTNVVAGAFSPTVFIGKDNTEKLVFSAYYKRRFTLYIADS
                     KKSVRRLPDLAPAVSPAGPESETPFQPAIEVSIDPEKIKPRASHKLQLEDAQVVAGVN
                     TDQTFVSDTVLVFGDNLGDRRLIAQFQSLSSFTNFNFVYFNLKSRIQKGFALFDDRTF
                     FLGFDQSRGTLERGRRIYRQTGALALASYPLSRYYRIEGQLGYISRKFDFPFVITNRD
                     GTQAFFVSARSDNYPTAGISFVGDTVLYENWGPLSGRRFSISYSYSPDFKKEDDPFTP
                     EVENSSTLSSDVSIDLRRYFKITPRSLVAIRFFGAHSTGNFPNVYYFGGLDTLRGLRF
                     REQIGNTVGYANFEYRFPLIDVLSTPILQFRDIRGQVFVDVGGAALKNQTFRFWDSNE
                     RQLVDGRAAYGMGFQIEFLGLQLHWDFAKLWDFKKTLSGLKTSFYIGAQF"
BASE COUNT          559 a          863 c         1017 g          547 t
ORIGIN      
        1 gggtgaccat gtcgtagtcg atgcgctcct gccccgcgag cggcggagca gcgaggatgg
       61 gaaggaggac gagaaggacg gcggcgcggg tgggcgagag tgggacggat ctcctcacgg
      121 acgcgcggag tatatggggt caggtctcca cttgccacat tgggggtcag gtctccactt
      181 gccacttcgc ttcgcggcgt ggcaagaccg gtctggctca acggccgaac ccggcgactc
      241 ttgaatacct gaccccgcta agaccttaaa actgcgcgcc gatgtagaaa gaggtcttca
      301 accccgacag cgttttcttg aagtcccaga gcttcgcgaa gtcccagtgg agctgcaggc
      361 ccagaaattc gatctggaac cccataccgt atgccgcccg gccgtcgacg agctgcctct
      421 cattggagtc ccagaaccgg aacgtctggt tcttcagcgc ggcgccgcct acatcgacga
      481 agacctggcc gcggatgtca cggaactgca ggatcggcgt cgagagaacg tcgatcaacg
      541 ggaaccggta ctcgaagttc gcgtagccga cggtgttgcc gatctgctcg cggaaacgga
      601 gaccccgcag cgtgtcgagg ccgccgaagt aatagacgtt cgggaaattg ccggtcgagt
      661 gggcgccgaa gaagcggatc gccaccagcg atcgcggcgt gatcttgaag taccgccgca
      721 ggtcgatgga cacgtccgat gagagcgtgc tcgagttctc gacctccgga gtaaagggat
      781 cgtcctcctt cttgaaatcc ggcgagtagg agtacgagat cgagaagcgg cgtccggaga
      841 gaggccccca gttctcgtag agcacggtgt cgccgacgaa ggagatgccc gccgtcgggt
      901 agttgtcgga ccgcgcggag acgaagaacg cctgggtccc gtcgcggttc gtgatcacga
      961 acgggaagtc gaacttccgg gagatgtagc cgagctgtcc ttcgatccgg tagtagcgcg
     1021 agagcggata gctcgcgagg gcgagagctc ccgtttgccg gtagatgcgg cggccgcgct
     1081 cgagcgtacc gcgcgactgg tcgaagccaa ggaagaacgt ccggtcgtcg aagagagcga
     1141 accctttctg gatgcggctc ttcaggttga agtagacgaa attgaagttc gtgaacgacg
     1201 aaagcgactg aaactgcgcg atcagccggc ggtcgccgag gttgtcgccg aagaccagca
     1261 cggtgtccga cacgaacgtc tggtcggtgt tgacgccggc gacgacctgg gcgtcctcga
     1321 gctggagctt gtggctggcg cggggcttga ttttttcagg gtcgatcgag acctcgatcg
     1381 ccggctggaa aggcgtctcg gactcgggtc ccgcgggaga aacggccggc gcgagatcgg
     1441 gcaggcggcg gaccgacttc ttcgagtcgg cgatgtagag cgtaaagcgc cgcttgtaat
     1501 acgcggagaa gacgagcttc tcggtgttgt ccttgccgat gaaaacggtg ggggagaagg
     1561 cgcccgcgac gacgttcgtg tgaaggttcg tctctccggt ctcgagattg atcgagtaga
     1621 tgttgaagat cccgccgtcc cggtcggagg tgaagaagag ccgcttcgag tcgggcgtga
     1681 gcgccgcgtc ctcgtcgttc cagtcgccat acgtgatctg ttggcgagag ccgggtgagg
     1741 tgggatcgaa ccggaaaatc ttcgccttcg tgccgcggac ggaggagtag tagatccact
     1801 ttccgtcggg cgagtaggtg ggcgcgaaat cgaacgccgc gtcgtcggtc aggttggtga
     1861 gggtcttcga agtcagggaa tacgcccaga tatccgcgtg cccgcccttg agcgcccgga
     1921 agacgaccgt gtttccatcc ggcgagaacg cggggttcag ctgctggtcg aggccgggca
     1981 tctcgatccg ctcgcggatt ccgccgtcga gagcattcag caggacgagc tcgcgcccgc
     2041 gctcgcgccg cgcgaataca ccgacggtgt tcccgtccgg cgcgacggca atgtcccctc
     2101 cgccgagcgg cccggtcgtc aaccactggc tgacgatgta ctcgtagcgg gtcgtgtagc
     2161 ctcgcgtgag gttgcgatag agcttccgat cctgcgtcga gaggacgacg acgtcggcgt
     2221 cgttcttcag cgtcgagatc gcggcgacga agtcgccgga cggatacgct cgcggggtga
     2281 gctcggccga cggctcctcg acgagcttga agcgctcgcc gaagtcgatc ggctcaccct
     2341 tctccgtcag gatcttcagg taccgctgcc gcaagtagcg ccggaacttg acgtcgaagt
     2401 cctcggccga gatgttgaac gcacgcttga tcaccttttc gacgtttccg ccgatctggt
     2461 tgcggaactc gaagacgaag tcgcggaccg agtccttgcc ccactccgcc tcgatgaagt
     2521 cgaacaccga gtggccgaac cggtaggcga agaaaccgct gatcgcgccg gtctgcgcga
     2581 tctccggcac ctggtcggcg aggaccgcgt cgcgcagaac cattcggtcc ttgttgtcct
     2641 cgtcgttccc gaagtacgac gccatcccct ccatgaacca ctggggcgcg ttcgtcgtgg
     2701 ccgcgcggat gtagttcccg ccgaagatga tctcgaactg gaagacgtgc gtgagctcgt
     2761 gcgcgatcag ctgctggagc gtctcgtcgg gcagatcgat cggcagcacc atccgattgc
     2821 gcgaggggag cgcgaacgcg ccgatcccct ccggaatgaa gttcaagatc gtattcgtct
     2881 gctcgaagtc cgagtggctt gcgtagtaga tgaggttgat cggcttgggg atctggaagt
     2941 tcaaggcgcg cgagatttcg tcgtaagcgc tctcggcgta cgacgt
//
LOCUS       DASWAV010000029         2655 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U2665
            k141_441792, whole genome shotgun sequence.
ACCESSION   DASWAV010000029 DASWAV010000000
VERSION     DASWAV010000029.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960316
            Sequence Read Archive: SRR10260007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2655)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2655)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:47:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,929
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2655
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_441792"
                     /isolate="SMAG_U2665"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.36444 N 132.31889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..293
                     /locus_tag="VF958_00575"
     CDS             <1..293
                     /locus_tag="VF958_00575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF3417 domain-containing protein"
                     /protein_id="HEX9147628.1"
                     /translation="GFVIGEATDESVGEQAANALYDALEERVVPLFFERDQDGIPRGW
                     IDKMVFSATRVARMFSSDRMVTEYLELCYLPAAERRLAAMNGRVKQFAPEEV"
     gene            290..1738
                     /gene="malQ"
                     /locus_tag="VF958_00580"
     CDS             290..1738
                     /gene="malQ"
                     /locus_tag="VF958_00580"
                     /EC_number="2.4.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007191633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004134 - 4-alpha-glucanotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0005980 - glycogen catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HEX9147629.1"
                     /translation="MTDRVAGILLHPTSLPGRFGIGDVGPMAERFLDWLASAGQSIWQ
                     VLPLGPTGYGASPYGSISSFAGNPLLISPEKLVEDGFAPASALDDVPDFPTDRVAWDG
                     VRDWKEKLFRLAWNRSSGSARVREDLEAFRRAPEQATWLADWSLFSARLSTGRAGERA
                     DLAEEVEYQEFLQLTFFRQWERLHGEARRRGISLFGDVPIYVAPGSVDIETHRELFAL
                     DAEGRALEVAGVPPDFFSETGQLWGYPLYRWDRMAEDGYAWWVERLRAAFRLTDIVRL
                     DHFRAFAAYWAVPAGETTAINGRWRPGPGRKLFDAVRKALGKVPLVAEDLGTITPDVK
                     RLLAKLGLPGMKVLQFAFSEADSQYLPHRHVSNSVVYTGTHDNDTTRGWWDTLDPDAR
                     GRVRDYLGSTGAEIEWDLIRAAYASVADRAIVPLQDVFGLGGEARMNTPALAEGNWSW
                     RAREEDFRNDRNARLRWLAELTGRLKKEPAGP"
     gene            1735..2631
                     /locus_tag="VF958_00585"
     CDS             1735..2631
                     /locus_tag="VF958_00585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014399147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEX9147630.1"
                     /translation="MSAAPRGRAFGKTGLTVPALGFGAGIAGDPSLSERNAEALLHSV
                     LDLGIGLIDTARSYGLSEERIGRYISRRRGEFVLSTKVGYGVPGHEDWTAGCVLPGVD
                     LALTKLRTDVLDLVHLHSCPRSTLEGSGVVEALARAVESGKVRVAAYSGDNDALEWAI
                     NSGAFGSVQFSLNVVDQAAVDTALPVARERGLGVIAKRALANAVWRRQPLASEDPAIS
                     TYRERWNALAIDLGGLPAEEVALRFTASLAGVHAVLVGSRRLEHLKENVEAISRGPLA
                     EGLERAIRLRFRERGAGWPGIV"
BASE COUNT          395 a          905 c          933 g          422 t
ORIGIN      
        1 cgggcttcgt catcggcgag gcgaccgacg aatcggtcgg agaacaggcg gcgaacgcgc
       61 tctacgacgc gctcgaggag cgggtcgtgc ctctcttctt cgaacgcgac caggacggaa
      121 ttccgagggg ctggatcgac aagatggttt tctccgcaac gcgcgtcgcg cggatgttct
      181 cgtcggatcg aatggtgacc gagtacctcg aactctgcta cctgccggcg gccgagcgac
      241 ggctcgcggc gatgaacggc cgcgtgaagc agttcgcgcc ggaagaggtg tgacagaccg
      301 ggtcgccgga atactccttc atccgacgtc cttgcccggc cgcttcggca tcggcgacgt
      361 cggaccgatg gccgagcgat tcctcgactg gctcgcgtcc gcgggtcagt cgatctggca
      421 ggtgctgccg ctcggtccca ccgggtatgg cgcatcgccc tacggctcga tctcgtcgtt
      481 cgccggaaac ccgcttctca tctcacccga aaagctggtg gaagacggct tcgcgcccgc
      541 ctccgctctc gacgacgtgc cggacttccc gaccgatcgg gtcgcctggg acggcgttcg
      601 ggactggaag gagaagctct tccgtctggc gtggaaccgc tcctccggct ccgcgcgcgt
      661 ccgcgaggac ctcgaagcct tccggcgcgc gcccgagcag gcgacctggc tcgccgactg
      721 gtcgctcttc tcagcgcggc tctcgaccgg ccgcgccggg gagcgcgcgg atctcgccga
      781 agaagtcgag taccaggagt tcctgcagtt gacgttcttc cggcagtggg aacggctaca
      841 cggggaggct cgccggcgag gcatctcgct cttcggagac gtaccgatct acgtggcgcc
      901 cggcagcgtg gatatcgaga cgcaccgcga gctcttcgcg ctcgatgccg agggccgcgc
      961 gctcgaagtc gcgggcgttc cgcccgactt cttcagtgag accggccagc tctgggggta
     1021 tccgctgtac cgctgggacc ggatggcgga ggacggatac gcatggtggg tcgagaggct
     1081 tcgcgccgcg ttccgcctga ccgacatcgt gcggctcgac cacttccgcg ccttcgcggc
     1141 ttactgggcg gtgcccgccg gcgagacgac cgccatcaac ggacgctggc ggccgggccc
     1201 ggggcgcaaa ctcttcgacg ccgtccgaaa ggcgctcgga aaggtcccgc tcgttgcgga
     1261 ggacctggga acgatcaccc cggacgtgaa gcgcctcctc gcgaagctcg gcctgccggg
     1321 gatgaaggtg ctgcagttcg cgttttccga ggcggacagc cagtacctgc cgcaccggca
     1381 cgtttcgaac tcggtcgtct acacggggac gcacgacaac gacacgacgc ggggctggtg
     1441 ggacacgctg gatccggacg cgcgaggccg ggtccgtgac tatctcggga gcacgggcgc
     1501 cgagatcgag tgggacctga tccgcgcggc gtacgcgtcg gtcgccgacc gagcgatcgt
     1561 gccgctgcag gacgtcttcg gcctcggcgg cgaagcgcgc atgaacacgc cggcgttggc
     1621 ggaaggcaac tggagttggc gcgcgcggga agaggacttt cggaatgacc gaaacgcccg
     1681 gctccgctgg ctcgcggaac tcaccggacg cctgaagaag gaaccggccg gaccttgagt
     1741 gccgcgccgc gggggcgcgc cttcggcaag accgggctga ccgtccccgc gctcggcttc
     1801 ggggccggca ttgcgggcga tccgtcgctc tcggagcgga acgcggaggc gctgctccac
     1861 tcggttctcg atcttggcat cgggctcatc gatacggcgc ggagctacgg cctctccgag
     1921 gagaggattg gccgatacat ctcccgccgt cgcggggagt tcgttctctc gacgaaggtc
     1981 ggctacggcg tcccgggaca cgaggactgg acggcgggct gcgtcctccc cggcgtcgac
     2041 ctcgctctca cgaaactccg gaccgacgtt ctcgacctcg tccacctcca ctcctgcccg
     2101 cgttcgacgc tcgagggcag cggcgtcgtc gaggcgctcg ctcgggcggt cgagtccggg
     2161 aaagtccgcg tcgcagcgta ctccggcgac aacgacgccc tcgagtgggc gatcaattcc
     2221 ggcgccttcg ggagcgtcca gttctccctg aacgtcgtgg accaggcggc ggtcgacacg
     2281 gcccttccgg tcgcccggga gcgcggtctg ggcgtcatcg ccaagcgggc cctcgcgaac
     2341 gcggtctggc ggcgccagcc gctggcctcg gaggaccccg ccatctcgac gtaccgggaa
     2401 cggtggaacg ccctcgccat cgatctcgga ggcctgcccg cggaagaggt cgctctccgt
     2461 ttcacggcaa gtctcgcggg agtccacgcc gtcctcgtcg ggtcgcgaag gctcgagcac
     2521 ttgaaggaga acgtcgaggc gatctcccgc ggtccgctcg cggaagggct cgagcgcgcg
     2581 atccgcctcc gcttccggga gcgcggggcg ggatggcccg gcatcgtctg accacccgcg
     2641 tgattcaatc gagcg
//
LOCUS       DASWAV010000030         3716 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U2665
            k141_601775, whole genome shotgun sequence.
ACCESSION   DASWAV010000030 DASWAV010000000
VERSION     DASWAV010000030.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960316
            Sequence Read Archive: SRR10260007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 3716)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3716)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:47:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,929
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3716
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_601775"
                     /isolate="SMAG_U2665"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.36444 N 132.31889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1174)
                     /locus_tag="VF958_00590"
     CDS             complement(<1..1174)
                     /locus_tag="VF958_00590"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HEX9147631.1"
                     /translation="MPLPAGTRVGPYEIVSPLGIGGMGEVYRARDPRLGREIALKVLP
                     EELSTDADRLARFEREARSASALNHPHIVTVYDIGRADSLSYIAMELVEGHTLREAVS
                     SGPLPGKRAVAIAAQAADALAKAHSVGIVHRDLKPENVMVSRDGFVKILDFGLAKLVA
                     HVSDTVSHAATAVDATRPGVLLGTVGYMSPEQARGGEVDFRSDQFSFGSILYEIASGR
                     RAFQGESAPETMAAIIREEPESLGRAQLSVPPPYRWIVERCLQKDPDERYASTRDLAR
                     ELADLRDHFSEIDRSGTSISTVALPAARPRGSRLLLPAIAVAAFALGIGAAALWSSRR
                     SPALPPAIRALTYSGADQSPAISPDGRTIAFTSRRDGTARIWVKQLATGGEAALTSG"
     gene            complement(1189..1734)
                     /locus_tag="VF958_00595"
     CDS             complement(1189..1734)
                     /locus_tag="VF958_00595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9147632.1"
                     /translation="MNREAVAEALTQDGEGLRVRWRGLALTFDEPTLNRLVARVAARI
                     PDLQDLRVHVRPGELSASLVVHRFGVPLSAKATLSQLRFKDGFLAFVLDKLQALSFIP
                     IPDQLLSYLVQKAPRGLLTYYPDDRIMVVNLSQWIPPGIDLSLERTVFAQGAMTLEFG
                     DGSYDLAPLIGEAAGGGADRD"
     gene            complement(1764..2753)
                     /locus_tag="VF958_00600"
     CDS             complement(1764..2753)
                     /locus_tag="VF958_00600"
                     /inference="COORDINATES: protein motif:HMM:NF013226.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiA family prenyltransferase"
                     /protein_id="HEX9147633.1"
                     /translation="MKRWIRLSRPFTLLPPLLGILSGAVCAFGSAHNPDPTRQVTWPI
                     VLTVMIGSVCASAMNAASNIVNQIADLEIDRRNKPDRPLVRGQVSIGSAWVVAAALYV
                     LAIVPTWLVVPYPYHSFAERMTAPIQLHAAFFIYCIGALATFVYSFPAFGRSKRHWFW
                     ANFTIAVTRGGLLKVAGWSFVASVALWEPWTIGSVFALFLLGATSTKDFSDMPGDREH
                     GVVTLPVRFGVGPAARLIAPFFVVPWLLVPLFAWLPDPDNPVEPLLTGDRRLLTLLGL
                     GLAAWGIYAARLLLRNPDELARTENHPAWTHMYLLLMSAQVGFAVAYIVSGLR"
     gene            complement(2750..>3716)
                     /locus_tag="VF958_00605"
     CDS             complement(2750..>3716)
                     /locus_tag="VF958_00605"
                     /inference="COORDINATES: protein motif:HMM:NF014049.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF92 domain-containing protein"
                     /protein_id="HEX9147634.1"
                     /translation="TLPWNRGKRWIGLAANWAAGATASILVFWFVSARPLDRLAAVLL
                     ILGAAVYAFLESVRAGIDDNLVAALPTALVLVQLGRWPEAPLAPFHSGGSGQLAAAFV
                     VNLAAGLVTWRSGLVSRSGALAGSLVGFLILAYGGWASYALLWTFFLAGTAATKWGYG
                     VKRDAGVAQADRGRRGAAHVTANCIVPVALLVLGTPAAAFAGALAAALADTLGTEVGS
                     LFGRDPRSPLTFDPVPKGTPGGVSLAGTGAALGGACLMALVAWIFGWLPRAAILAVIV
                     GGFLGALAESFLADVRRRSGGRLDHEFANAFNTFAGALIALGLSR"
BASE COUNT          699 a         1224 c         1317 g          476 t
ORIGIN      
        1 gcccggaggt gagggccgcc tcgccgccgg tcgcgagctg cttgacccag atccgcgcgg
       61 ttccatctct ccgcgaggtg aacgcgatcg tccgcccgtc cggcgagatc gcgggggatt
      121 gatccgcgcc cgagtaggtg agcgcgcgga tcgcgggcgg cagtgccggg ctccggcgcg
      181 acgaccacag ggcggccgcc ccgattccga gcgcgaacgc ggcgaccgcg atcgccggca
      241 ggaggagccg cgatccgcga ggtcgagccg ccgggagcgc cacggttgag atggaggtcc
      301 ccgagcggtc gatttcggag aagtggtccc gcaggtccgc gagctcccgg gcgaggtcgc
      361 gcgtcgaagc gtagcgttcg tccggatcct tctgaagaca tcgttcgacg atccagcgat
      421 acggcggggg cacagacaac tgggcgcgac ccagcgactc cggctcctcc cggatgatcg
      481 cggccatcgt ctcgggcgcg ctctcgccct ggaaggcgcg gcgccccgac gcgatctcgt
      541 agaggatcga accgaacgag aactggtccg accggaagtc gacctctcct ccgcgcgcct
      601 gctccggcga catgtatccg acggtgccga gcaggacgcc cgggcgcgtc gcgtcgaccg
      661 ccgtcgcggc atgcgagacc gtgtcggaaa cgtgcgcgac gagcttcgcg aggccgaagt
      721 cgaggatctt cacgaaaccg tcgcgcgaga ccatgacgtt ctcgggcttc agatcccggt
      781 ggacgattcc caccgagtgc gccttggcca gggcgtctgc cgcctgtgcc gcgatcgcga
      841 cggcgcgctt gcccggaagc ggcccggaag agacggcctc gcgtagagtg tggccctcga
      901 cgagctccat ggcgatgtac gagagcgagt cggcgcggcc gatgtcgtac accgtgacga
      961 tgtgggggtg gttcagcgcg gacgccgagc gcgcctcgcg ctcgaacctc gcgagccggt
     1021 ccgcgtcggt cgacagctcc tcgggcagaa ctttcagggc gatctcgcgg cccaaccgcg
     1081 gatctcgggc gcgatagacc tcccccatgc cgccgatacc gagcggagag acgatctcgt
     1141 atggcccgac tcgagttccc gctggaaggg gcatttcgaa gcgagagtct agtctcggtc
     1201 ggctcccccg ccggcagctt ccccgatcaa cggcgcgagg tcgtacgaac cgtcgccgaa
     1261 ctcgagcgtc atggcgccct gggcgaagac ggtcctctcg agcgaaaggt cgatgcccgg
     1321 cggaatccat tgcgacaggt tgaccaccat gatgcgatcg tcggggtaat acgtgagcag
     1381 tcctcgcggc gccttctgga cgagatacga cagcagctgg tcggggattg gaatgaacga
     1441 cagggcctgc aacttgtcga gcacgaacgc gaggaacccg tccttgaagc gcagctgcga
     1501 aagcgtcgcc ttcgcggaca gcggaacgcc gaaccggtgc acgacgagcg acgcggagag
     1561 ctcgccgggg cggacgtgga ctctcaagtc ctgcaggtcg ggaatgcgcg ccgcgacacg
     1621 cgcaacgagc cggttcaacg tcggctcgtc gaacgtgagc gccagcccgc gccagcggac
     1681 ccgcagtccc tcgccgtcct gtgtgagcgc ctcggcgacc gcttcccgat tcacgtgtcg
     1741 attgtacgcg ggcccgggct cggtcagcgg agaccggaca caatgtaagc caccgcgaac
     1801 ccgacttgcg ccgacatcag gagcaggtac atgtgcgtcc acgcgggatg gttttcggtc
     1861 cgcgcgagct cgtctggatt ccgcaggagg aggcgggcag cgtaaatccc ccaggccgcc
     1921 agtccgaggc cgagcagcgt caggagccgg cgatcgccgg tcaacagagg ttcgacagga
     1981 ttgtcgggat cgggcagcca ggcgaagagc ggaacgagga gccagggaac gacgaaaaac
     2041 ggcgcgatca gtcgcgccgc cggaccgacg ccgaaccgca cgggcagcgt cacgactccg
     2101 tgctcgcggt cgccaggcat gtcggagaag tccttggtcg acgtcgcgcc gaggagaaag
     2161 agcgcgaaca cgctcccgat cgtccacggt tcccacagag ccacggacgc gacgaaggac
     2221 cacccggcaa ccttgaggag tcctccgcgg gtgaccgcga tcgtgaaatt cgcccagaac
     2281 cagtgtcgct tcgagcggcc gaacgccggg aaggaataga caaacgtcgc gagcgcaccg
     2341 atgcagtaaa tgaagaaggc ggcgtgcagt tggatcggcg cggtcatgcg ctcggcgaac
     2401 gaatggtacg ggtacggcac gacgagccac gtcggaacga tcgcgaggac gtagagagcg
     2461 gcggcgacca cccaggcgct cccgatcgaa acttgtccgc gaacaagagg acggtcgggc
     2521 ttgttgcgcc gatcgatctc gaggtcggcg atctggttga cgatgttcga tgccgcgttc
     2581 atggcggatg cgcagaccga accgatcatc accgtcaaga cgatcggcca cgtgacctgg
     2641 cgggtcggat cgggattgtg ggcggagccg aacgcgcaca ccgccccgga gaggatcccc
     2701 agaagaggcg gcagcagcgt gaacggacgg gagagccgga tccagcgctt caccgggaga
     2761 gcccgagcgc gatgagagca ccggcgaacg tgttgaaggc gttcgcgaac tcgtggtcga
     2821 gtcgcccgcc cgaccgccgt cggacgtccg cgagaaagct ctcggcgaga gcgccgagga
     2881 atccaccgac gattacggca agaattgccg ctcgcggcag ccagccgaag atccacgcga
     2941 cgagagccat gaggcacgca cccccgagcg ccgcgccggt gccggcaaga gacacgccgc
     3001 cgggagttcc cttcggcacc ggatcgaacg tgagaggcga ccggggatca cggccgaaaa
     3061 ggcttcccac ctcggtgccg agcgtgtctg cgagcgcggc ggcgagcgcc ccggcaaagg
     3121 cggccgcggg agtcccgaga acgagaagcg ccacgggcac gatgcagttc gccgtcacgt
     3181 gcgccgcgcc gcgccggccg cgatcggcct gtgcgactcc cgcgtcgcgt ttcactccgt
     3241 agccccactt cgtcgcggcg gtgccggcga gaaagaacgt ccagagcaga gcataggagg
     3301 cccagccgcc gtacgccagg atgagaaagc ccacgagcga ccccgcgagc gctcccgagc
     3361 gcgaaaccag cccggaccgc catgtgacga gacccgcggc gaggttcacc acgaacgcgg
     3421 cggcgagctg tcccgatccg cccgaatgga agggggcgag cggagcttcg ggccagcgcc
     3481 cgagctggac gagcacaagc gccgtcggca gtgccgcgac caggttgtcg tcgatacctg
     3541 ccctcacgga ctcgagaaac gcgtagacgg cagcgccgag aatgagcaac acggcggcga
     3601 gacgatcgag cggccgcgcg gaaacgaacc agaacacgag gatggaggcg gtcgcgccgg
     3661 cggcccagtt cgcggcgaga ccgatccagc gcttgccgcg gttccaggga agcgtc
//
LOCUS       DASWAV010000031         2274 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U2665
            k141_121261, whole genome shotgun sequence.
ACCESSION   DASWAV010000031 DASWAV010000000
VERSION     DASWAV010000031.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960316
            Sequence Read Archive: SRR10260007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2274)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2274)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:47:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,929
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2274
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_121261"
                     /isolate="SMAG_U2665"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.36444 N 132.31889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..350
                     /locus_tag="VF958_00610"
     CDS             <1..350
                     /locus_tag="VF958_00610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020720745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="pilus assembly protein PilB"
                     /protein_id="HEX9147635.1"
                     /translation="LVRKICIHCREKVEVSPQAILEAGLDPASVVGQIFYEGRGCLEC
                     DGTGFHGRLAIAELLDLSDRIRGLILERRPAAEIKKAAKEEGMTFLREAALAKVFEGS
                     TTLKEINKVTFVD"
     gene            350..1273
                     /locus_tag="VF958_00615"
     CDS             350..1273
                     /locus_tag="VF958_00615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9147636.1"
                     /translation="MRIPLLPPVVPSHVFCLMAESVTWANVRREPTPGFVESRSFVYP
                     PNSLGAGPSGTPLFSREALADAVDAARKLSGGRLSKASVVFPDSWARILPIDFDTLPE
                     SQEGARDMVLWKLKKLLPGISEELSVVFEEMPRAGDGEIRLLVAAAPTESLHSIEQSF
                     ETLGVRVGALSPASLALFGGLAPVLGPLSGGDYGLIHRTPGSFIFVIARDGSPVFFRQ
                     RPEEEADGDQDQEVRLSLSYYLEKLHGTGLSAVYVHDAVAGGVLASASALPVKPTPIT
                     GKLFSADRGFDERVAAKPELLPAFAAVYGRG"
     gene            1278..1862
                     /locus_tag="VF958_00620"
     CDS             1278..1862
                     /locus_tag="VF958_00620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9147637.1"
                     /translation="MAATNFNFARRPFRDFRPVYVVAGGALAAGLILLAVNASLYGEF
                     RREVSDTRDEIAWLEKRKQTATAAAEQARGAMRSYELAQLAGESRGLRRLVEERQFSW
                     MAFLGRLERVLPAEVRVSRLTPRFQESGEVSIDVGLLGRGPESVVRTISALSRSPYFS
                     AVQLSSESYPESKSGVPEGHTFIITTVYAPEAAP"
     gene            1859..>2274
                     /locus_tag="VF958_00625"
     CDS             1859..>2274
                     /locus_tag="VF958_00625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9147638.1"
                     /translation="MKGRENVWRRRAGLLAVATLFLLANFGFLVGSRSIRAARKQALE
                     ARRSGLTSEVTALEAEAKRLVEQRDRLAQVSSAIEEFYGRRVGSQRETLAPVVEEIHA
                     VMKKNGVAPSSISYTTAPLPSLPLSLMSIGFSFKSD"
BASE COUNT          339 a          766 c          774 g          395 t
ORIGIN      
        1 gtctcgtccg gaagatctgc atccactgcc gcgagaaggt cgaggtgtcg ccccaggcga
       61 tcctcgaagc cgggctcgat ccggcgtcgg tcgtcggtca gattttctac gagggacgcg
      121 ggtgtctcga gtgcgacggc accggcttcc acggacgcct ggcgatcgcg gagctcctcg
      181 acctgtccga ccggatccga gggctgatcc tcgagcggcg tcccgccgcg gaaatcaaga
      241 aggcggcgaa ggaggagggc atgaccttcc tcagggaagc ggcgctcgcg aaggtgttcg
      301 agggctcgac gacgctgaag gaaatcaaca aggtcacgtt cgtcgactga tgcgaattcc
      361 gctgctgccg ccggtcgttc cttcccacgt cttctgcctg atggcggaga gcgtgacgtg
      421 ggcgaatgtc cgccgcgagc cgacgccggg attcgtcgag tcgcgcagtt tcgtgtatcc
      481 gccgaattcg ctgggcgccg gaccgtcggg aactccgctc ttctcccgcg aggcccttgc
      541 cgacgccgtc gacgcggcgc gcaagctctc gggcgggcgc ctctcgaagg cgtcggtcgt
      601 ctttcccgac tcctgggcgc gcatcctccc gatcgacttc gacacgctcc ccgagtcgca
      661 agagggcgcg cgcgacatgg tcctctggaa gctgaagaag ctcctccccg gaatcagcga
      721 ggagctttcc gtcgtgttcg aagagatgcc acgagccggc gacggcgaga tccgcctcct
      781 cgtggccgcc gcgccgaccg aatctctcca ctcgatcgag cagtcgttcg agaccctcgg
      841 agtccgggtg ggcgccctct ccccggcgag cctcgccctg ttcggggggc tcgcccccgt
      901 gctcggacct ctctccggcg gtgactacgg gctcattcac cgcaccccgg gatcgttcat
      961 cttcgtcatc gcgcgcgacg gcagcccggt cttcttccgg cagcgacccg aggaggaggc
     1021 ggacggggac caggatcagg aggtccggct ctcgctctcg tactacctcg agaagctcca
     1081 cggcaccggt ctctcggcgg tctacgttca cgacgccgtt gcgggcggtg tactcgcgag
     1141 cgcgtcggcg ctcccggtca agccgactcc gatcaccgga aagctgttct ccgcggaccg
     1201 gggcttcgac gagcgcgtgg cagcgaagcc cgagctcctt cccgccttcg cggcggtcta
     1261 cggaaggggt tagccgggtg gccgccacga acttcaattt cgcgcggcgg ccgttccgcg
     1321 acttccggcc cgtttacgtg gtggcgggcg gcgcgctcgc ggcgggcctg atcctcctcg
     1381 cggtcaacgc gagcctttac ggggagtttc gccgcgaggt ctccgacacg cgagacgaga
     1441 tcgcgtggct cgaaaaacgc aagcagaccg ctacggcggc ggccgaacag gcccgcggag
     1501 cgatgcgctc gtacgagctc gcccagctcg ccggcgagag ccgcggattg cggcgtctcg
     1561 tcgaggaacg gcagttctcg tggatggcgt tcctcggacg gctcgagcgg gttctccccg
     1621 cggaggttcg tgtctcccgt ctcacgccgc gattccagga gtcgggagaa gtctcgatcg
     1681 acgtcggtct tctcggccgc ggtccggaga gcgtcgtccg cacgatctcc gcgctctcgc
     1741 gatcgccgta cttttcggcg gtccagttgt cgtcggagtc gtacccggag tccaagtccg
     1801 gcgtgccgga aggccacacg ttcatcatca ccaccgtgta cgcgccggag gcggcgccat
     1861 gaagggacgc gagaacgtgt ggcggcggcg cgcgggcctt ctcgccgtgg caacgctgtt
     1921 cctcctcgcg aacttcggat tcctggtggg ttcccgatcg atccgggcag cccgcaagca
     1981 ggcgctcgag gcgcggagat ccggcctgac gagcgaggtc accgcgctcg aggcggaggc
     2041 gaagaggctc gtcgaacagc gggaccggct ggcgcaggtt tcttcggcga tcgaggagtt
     2101 ctacggtcgc cgggtcggct cgcagcgcga aacactggcg cccgtcgtgg aggaaatcca
     2161 cgcggtcatg aaaaagaacg gcgtcgcgcc gagttcgatc agctatacga cggctccgct
     2221 cccgagcctg ccgctttccc tgatgtcgat tggcttctcg ttcaagagcg acta
//
LOCUS       DASWAV010000032         5436 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U2665
            k141_361948, whole genome shotgun sequence.
ACCESSION   DASWAV010000032 DASWAV010000000
VERSION     DASWAV010000032.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960316
            Sequence Read Archive: SRR10260007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 5436)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5436)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:47:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,929
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5436
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_361948"
                     /isolate="SMAG_U2665"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.36444 N 132.31889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(321..608)
                     /locus_tag="VF958_00630"
     CDS             complement(321..608)
                     /locus_tag="VF958_00630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9147639.1"
                     /translation="MTKSDALPGTYRVDREGYAFRLDAAGVRAVKALPDYEGKEEPAV
                     AEEFLKTRVEAWVDALASAGAERGVYDVRVDEHQGKAHLDREGLLVFSADL"
     gene            650..1306
                     /locus_tag="VF958_00635"
     CDS             650..1306
                     /locus_tag="VF958_00635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9147640.1"
                     /translation="MFSARFASRIALLVLLVAAPAFAEKLRMHFDADSAGRPPGFFDL
                     LVWGAPGEAEWLVLGDVNPPSTPNKLIQTIDTRPVGSIAVALRRNYVFQDGDISVGLR
                     RGSGQAGIVFRAAGEKNFLALLVNVASGEAQLSSWVGGKATELARGKAEIDHDWGTLV
                     VTASGPAVTARWNGKPLLTATDPHPIPGRVGLATAGPGQASFDELVFDGSMSAPAPAR
                     "
     gene            complement(1307..2467)
                     /locus_tag="VF958_00640"
     CDS             complement(1307..2467)
                     /locus_tag="VF958_00640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002633338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX9147641.1"
                     /translation="MTGRRILGLIPTLAALSFLAGCGAEKPPGAEGIRVGFFGALTGP
                     TATFAQSGRNGVQMAVEELNAAGGVLGGKHIELLVEDDRGEASEAASAVSKLITRDHV
                     VALIGEQASSRTIAAAPIAQSYGVPMISPTSTSADVTKKGDYIFRVCFTDPYQGAVLS
                     SFARENLKANTAAQLVDVRNDYSVGLAQAFRESFEKAGGRILAEQNYSEGDSDFSAQL
                     TAIRPLDPDVLVVPGYYTDAGLIARQAKALGIRATLLGGDGWDSPKLTEIGGDTVEGA
                     YFSNHYSVDDPSPTVQQFVAAYRKKYGANPDSIAALSYDAARLLADAIRRAGSTEGKR
                     MRDALAETKDFSGVSGMIKMDADRNPIKPAVVLKVEGGRFRFVTAVSPKAGQ"
     gene            2509..3918
                     /gene="lpdA"
                     /locus_tag="VF958_00645"
     CDS             2509..3918
                     /gene="lpdA"
                     /locus_tag="VF958_00645"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012258694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA];
                     GO_process: GO:0051068 - dihydrolipoamide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HEX9147642.1"
                     /translation="MEAPKTYDVVIIGSGPGGYVAAIRAAQLGMSILIVERDPKLGGV
                     CTLRGCIPTKALLHTADVLEEARHGTEVGVATREVRLDFPAAMKHKEKVVRQSTNGIS
                     YLMKKNKVDVVAGFGRIAGPGKVSVAGPDGAETTYSAKNIIVATGSTPRSLPGIEIDH
                     QAILSSDSMLELAEVPKSLLVIGSGAVGVEFASMYSRFGSKTTVVEILPRIVPLEDEE
                     VSRELAASFKRQGIAVYVDTRVERVSKSDSGVEVIARSSGGTKTETFRAEKILLAVGR
                     KPLSENIGLEGLGVETDRGYVKVDKFMRTNVPNVYAIGDVVPTPWLAHVASAEGVVAV
                     EHMARGETRPLNYDQIPGCTYCAPEVASIGLSEAAARERGHDVVVGKFPFSASGKARI
                     VNETAGFVKIVAEKKYDEVLGVHIIGPKATELIAEAGVALKLEATSEELVRTIHAHPT
                     LAEVIHEAAEAVSGHAIHI"
     gene            complement(4026..4418)
                     /locus_tag="VF958_00650"
     CDS             complement(4026..4418)
                     /locus_tag="VF958_00650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9147643.1"
                     /translation="MSKRLAVAGIVFACAFALGAEETLYQIDLVPSGKLVSRDLPVLK
                     GTTYVFHEFPRGTLISVRKSTVKQITRMSPRAAALVNPKTRLVRIGDLAFQGPRSGLT
                     GGNAPSNMGRARDAVSAANAGTAARTGN"
     gene            complement(4487..5053)
                     /locus_tag="VF958_00655"
     CDS             complement(4487..5053)
                     /locus_tag="VF958_00655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9147644.1"
                     /translation="MNLFARSLFAITIFPTVFAGAAEPNLYRIELAGNVVLFARTPPV
                     PRGGRLVYSRYPDGALMSLKRADVQRVVVTPVMKADSRNLKPGELLVLGPTGEGASSA
                     APAAGSGAATRPGEAPGGKALFNPSRDYRPDWDSKQVPGQNLAYPASPGDYREGATMA
                     YPPGNATQSGSGQPPTGVPSGEPPKSPR"
     gene            5171..>5436
                     /locus_tag="VF958_00660"
     CDS             5171..>5436
                     /locus_tag="VF958_00660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9147645.1"
                     /translation="MGGFRDMNQEISRPAPDLAALRIRREPEKTRGPKAWLIGSAVLV
                     VAIGVAAYFFAGRSLAPKKVEVVTAALLTEGQATTVLTATGYIEA"
BASE COUNT          878 a         1837 c         1830 g          891 t
ORIGIN      
        1 gcgccactgg cgctcggctc ggcaagccag cctcgaccga gcgcctgatc cctcgctgcg
       61 ctcgcgctcc cgcggcgcgc gcctcctccc tcgattacct cgggagactt cgcgcgccgc
      121 gataggaagg gtgttctacc gatcaaagcc gtcgcggtcg cttgctcggg atctggctgc
      181 gccactggcg ctcggctcgg caagccagcc tcgaccgagc gcctgatccc tcgctgcgct
      241 cgcgctcccg cggcgcgcgc ctcctccctc gattacctcg ggagacttcg cgcgccgcga
      301 tgggaagggt gttccaccga tcaaaggtcg gcgctgaata ccagcaagcc ttcccgatcg
      361 aggtgcgcct tcccctggtg ctcgtcgacc cggacgtcgt agacaccgcg ctcggcgccc
      421 gccgaagcga gcgcgtcgac ccacgcttcg acgcgagtct tcagaaactc ctcggcgacg
      481 gccggctcct ccttgccttc gtaatcgggg agcgccttga ccgcccggac acctgcggcg
      541 tcgaggcgga aggcatagcc ctcgcggtcg acgcgatagg tccccggcaa agcgtcggac
      601 ttcgtcatgg tcgggaattt tagcggtgcg ccgccgttac tcctgcacga tgttttccgc
      661 cagattcgcc tcgaggatcg ccctcttggt acttctcgtg gctgctccgg cgttcgccga
      721 gaagctgcgg atgcacttcg atgcggattc cgcgggtcgg ccgcccggct ttttcgatct
      781 cctggtgtgg ggcgcccccg gcgaggccga gtggctcgtc ctcggcgacg tcaatcctcc
      841 ctccactcca aacaagttga ttcagacgat cgacacgcgc cccgtcggct cgatcgcggt
      901 ggccctgcgc cgcaactacg ttttccagga cggtgacatc tccgtgggct tgcgccgggg
      961 cagcggacag gccggaatcg tcttccgcgc cgcgggggag aagaacttcc tcgctcttct
     1021 ggtcaatgtc gcaagcggag aggcgcagct ctcttcgtgg gtgggcggca aggcgactga
     1081 gctcgcccgc ggtaaagcag agatcgatca cgactggggc acgctcgtgg tgaccgcctc
     1141 gggcccggcg gtgaccgcgc gctggaacgg gaagcctctc ttgacggcga ccgatccgca
     1201 cccgattccc ggaagagtcg gtctcgccac tgcgggcccg ggacaggcct ccttcgacga
     1261 gctcgtgttc gacgggtcga tgtcggctcc cgcacccgct cgatagctac tggcctgcct
     1321 tcggcgagac ggcggtgacg aaccggaacc gcccgccctc gactttcagc acgaccgcag
     1381 gcttgatcgg gtttcggtcc gcgtccatct tgatcattcc gctcaccccc gagaagtctt
     1441 tcgtctcggc gagcgcgtcg cgcatgcgct tgccttccgt cgagccggct cggcggatcg
     1501 cgtcggcgag gagccgcgcg gcgtcgtagg acagcgcggc gatcgagtcg gggttggcgc
     1561 cgtacttctt ccggtacgcg gcgacgaact gctgcaccgt gggggaagga tcgtccaccg
     1621 agtagtggtt cgagaaatat gccccttcga ccgtatcgcc cccgatctcc gtcagcttcg
     1681 gcgagtccca gccgtctccc ccgaggaggg tcgcgcggat cccgagcgct ttcgcctggc
     1741 gcgcgatcag accggcatcc gtgtagtagc cggggacgac gaggacgtcg ggatcgagcg
     1801 gtcggatcgc cgtgagctga gcggagaagt cgctgtcgcc ctcggagtag ttctgctccg
     1861 cgaggatgcg tccccccgcc ttttcaaacg actcgcgaaa tgcctgcgcg agtccgacgc
     1921 tgtagtcgtt gcgcacgtcc acgagctgcg ccgcggtatt cgccttcagg ttctcgcgtg
     1981 caaacgacga gaggacggcg ccctggtacg ggtcggtgaa gcagacgcgg aagatgtagt
     2041 cacccttctt ggtcacgtcc gcgctggtcg acgtcggtga gatcatcggg acaccgtagc
     2101 tctgcgcgat gggcgcggcg gcgatcgtgc gcgacgacgc ctgctcgccg atcagagcga
     2161 cgacgtggtc gcgcgtgatg agcttggaga cggcggaagc cgcctcgctc gcttcgccgc
     2221 ggtcgtcctc gaccagaagc tcgatgtgct tgccgccgag cacaccgccg gcggcgttca
     2281 actcctccac cgccatctgc acgccgtttc gccccgactg cgcgaacgtc gccgtcggtc
     2341 cggtcagcgc gccgaagaag ccgacccgga tcccctcggc tcccggcggc ttctccgctc
     2401 cgcagccggc gagaaaagag agtgcggcga gcgtcggaat gagaccaaga atgcggcgcc
     2461 cggtcactcg cggattatac ggagacggct gataggctac cgttccgcat ggaagcaccg
     2521 aaaacgtacg acgtcgtgat catcggctcc ggaccggggg gctacgtcgc cgcgatccgg
     2581 gcggcgcagc tcggaatgtc gatcctgatc gtggaacgcg acccgaagct cggaggcgtc
     2641 tgcacgctgc gcggctgtat cccgacgaag gcgcttctcc acacggcaga cgtgctggaa
     2701 gaagcgcggc acggcaccga ggttggcgtg gcgacgcgcg aggtccggct cgatttcccc
     2761 gcggccatga aacacaagga gaaggtcgtc cggcaatcca cgaacggcat ctcctacctc
     2821 atgaagaaga acaaggtcga cgtcgtggcc ggcttcggcc gcatcgccgg gccggggaag
     2881 gtgtcggtcg ccgggccgga cggcgccgag acgacctatt cggcgaagaa catcatcgtc
     2941 gccacgggtt cgacgccgcg gtcgctcccg gggatcgaga tcgatcacca ggcgatcctg
     3001 tcctcagact cgatgctcga gctcgccgag gttccgaagt cgctgctcgt catcggttcc
     3061 ggcgccgtcg gcgtcgagtt cgcctcgatg tactcgcgtt tcggctcgaa gacgacggtg
     3121 gtcgagatcc ttccgcgcat cgttccgctc gaggacgagg aggtctcgcg agagctcgcg
     3181 gcctccttca agcgacaggg gatcgcggtc tatgtcgaca cccgcgtcga gcgcgtctcg
     3241 aagagcgaca gcggcgtcga ggtcatcgca aggtcctccg gcggcaccaa gaccgagacc
     3301 ttccgggccg agaagatcct tctcgcagtc gggagaaagc cgctctcgga gaacatcggg
     3361 ctcgagggac tcggcgtcga gaccgaccgc ggctacgtca aggtcgacaa gttcatgcgt
     3421 acgaacgtgc cgaacgtgta cgcgatcggc gacgtcgtgc cgacgccctg gctcgcgcac
     3481 gtcgcgtcgg ccgagggagt cgttgccgtc gagcacatgg cgcggggcga gactcggcct
     3541 ctgaactacg accagatccc cggctgcacc tactgcgcgc cggaggtggc atcgatcggg
     3601 ctgtcggagg ccgccgcgcg ggaacgcggc cacgacgtcg tcgtcggcaa gttcccgttc
     3661 tcggcgagcg gcaaggcgcg catcgtgaac gagaccgccg gtttcgtgaa gatcgtcgcc
     3721 gagaagaagt acgacgaggt tctgggcgtg cacatcatcg gtccgaaagc cacggaactg
     3781 atcgcggagg cgggggtggc gctgaaactc gaggcgacct ccgaggagct cgtgcgcacg
     3841 atccacgcgc accccacgct cgccgaggtg atccacgagg ccgcggaagc ggtctcggga
     3901 cacgccatcc acatctagtc gagtgcgctc gcgaagcgct ggcccccgtc ccaggaactc
     3961 cctctccctc cgggagaagg gcgggtgagg gcagagctcc agccggaatc ggtgagaccg
     4021 tcccgctagt tcccggttcg tgcggccgtg cccgcgttcg ccgccgaaac cgcgtcgcgg
     4081 gcgcgaccca tgttggacgg cgcgttcccg cccgtgagac ccgagcgtgg cccctggaac
     4141 gcgagatcgc cgatccggac gagtctcgtc ttcggattca ccaaagccgc ggccctcgga
     4201 gacatgcggg tgatctgttt cacggtcgac ttgcgaacgc tgatgagcgt gcccctggga
     4261 aactcgtgga agacgtaggt cgtccccttc agcaccggga ggtcgcggga gacgagcttg
     4321 cccgacggga cgaggtcgat ctggtagagg gtttcttccg cgccgagcgc aaacgcacaa
     4381 gcgaaaacaa ttccggcaac cgcgagacgt ttcgacatga gctgacgccc accttcgaag
     4441 gtaccactct tctcgccgga ggatccggca cccgagtcgc gaccgcctat cgcggcgact
     4501 tcggcggctc cccgctcggg acgcccgtcg gcggctgtcc cgaaccggac tgcgtcgcgt
     4561 tcccaggggg atacgccatg gtcgcgccct cgcgatagtc gccgggtgaa gccggatacg
     4621 cgaggttctg cccggggacc tgtttcgaat cccagtcggg ccggtaatcg cgagaggggt
     4681 tgaacagcgc cttgccgccc ggcgcctctc ccgggcgagt ggccgccccg ctcccggccg
     4741 cgggcgcggc gctcgaggcc ccttcgccgg tcgggccgag caccagcagc tcaccgggct
     4801 tcaggttccg cgagtccgcc ttcattacgg gggtgacgac cacccgctgc acgtcggcgc
     4861 gcttcaagct catcagagcg ccatcgggat accgggaata cacgagacgg cccccccggg
     4921 gaacgggagg cgttctggcg aacagaacga cgtttccggc gagctcgatc cgatagagat
     4981 tcggctccgc tgcgccggcg aaaacggtcg ggaagatcgt gatcgcgaag agcgagcgcg
     5041 cgaagagatt catatccgaa attgttgcac gatccgggcc gctcgcgccg accggcagac
     5101 ggtgcttgcg cgccggcgag cgacgcaacc cggcggggac aggccgcgta gaggacgtcg
     5161 aatgaataga atgggcggct ttcgagacat gaaccaggaa atctcccgtc ccgcgcccga
     5221 tctcgcggcc ttgcgcatcc ggcgcgagcc ggagaagacc cgtggcccga aggcgtggct
     5281 gatcggatcc gccgtcctcg tggtcgccat cggcgtcgcc gcgtacttct tcgcgggccg
     5341 ctcgcttgcg ccgaagaagg tcgaggtcgt gaccgcggct ctcctgacgg aaggtcaggc
     5401 gacgaccgtc ctcacggcga cggggtacat cgaagc
//
LOCUS       DASWAV010000033         5517 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U2665
            k141_121679, whole genome shotgun sequence.
ACCESSION   DASWAV010000033 DASWAV010000000
VERSION     DASWAV010000033.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960316
            Sequence Read Archive: SRR10260007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 5517)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5517)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:47:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,929
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5517
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_121679"
                     /isolate="SMAG_U2665"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.36444 N 132.31889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1224
                     /locus_tag="VF958_00665"
     CDS             <1..1224
                     /locus_tag="VF958_00665"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006635 - fatty acid beta-oxidation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase family protein"
                     /protein_id="HEX9147646.1"
                     /translation="TLLKDVPAQELARIVTTELLARTGFDPAELDHVIFGNIAQPPDA
                     VNLSRGAALLAGVPRRVPAFTVNRLCGSGLQSIADAWYRISSGDADAIIAGGVESMSN
                     IPFLYSRESQEIFTEVFTAKDLGKRVAAASRFRPKHFKPEIGLQQGLTDAVCGLNMGE
                     TAEVLAKEHHISREEQDAFALKSHRRVTLARSKLAEEIVPVPIPPKYSDMATADNGVR
                     ENQTMEALGKLRPYFDPKFGTVTAGNSSQITDGGAATLVMSAEAASAHGYRPLGKVRG
                     FAFVGVDPERMGLGPAAATPLALKRAGVSWGDIGLVEMNEAFAAQCLAVARVFPSREW
                     HERYGTGGPIGEIDWERTNVNGGAIALGHPVGSSANRLVTTLLKEMRRRDVPLGLATM
                     CIGGGQGGSVVLEAM"
     gene            1221..3386
                     /locus_tag="VF958_00670"
     CDS             1221..3386
                     /locus_tag="VF958_00670"
                     /inference="COORDINATES: protein motif:HMM:NF012596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase NAD-binding
                     domain-containing protein"
                     /protein_id="HEX9147647.1"
                     /translation="MSAFGLDVGPDGLGLLTFDLPGEKVNKFSREVFVELSEVLVRLS
                     QDPRVRALLVVSGKPDVFIAGADVKEFTQVKPEDASAGSARGQALFEQLARLPFPTVA
                     AINGVCLGGGTEFALACDWRVMSDAASARIGFPEVRLGIFPAWGGCVRLPRLVGLANA
                     LDLILTSKQIDARRAKRIGLVDEAVPAAIFDDFARRFARSKIGAGKRAPRPTRLSSGE
                     RALEATSIGRRLIFKKARQNVIEQTGGHYPAPLEALAVVTETFGMPVEAALEVESRRI
                     ANLFGGEVQRNLLDIFFATEQVKRETGVDDPGVRPRGVTHVGILGAGVMGGGIAQLAA
                     DRGLPARMKDIEPKALAHGYAAAASVWKEALGRHRLTPREMTVRMARLSGTLDYSGFA
                     QCEVTIEAVVEKLAVKQAVLAEWEHAVPETAIFGSNTSTIPIGEIGASARHPERVAGM
                     HFFNPVHRMPLVEVIRGGKSSDEAVATIFALAKKLGKTPVVVRDMPGFLVNRILAPYL
                     SEAVRLVREGSRIEDVDRTMKDFGMPVGPLALLDDVGLDVAVKAGEVLQAAFPQRIKS
                     GGDEALVAEGRLGRKSGGGFYEYAGGKRGAPSGKAYEILRAQPSSSPRFSAEVIESRL
                     VLPMVNEAAFCLEDGVVPGAGKLDLAMIMGTGFPPFRGGLLKWADSLGLPRVRSLLEE
                     LTERFGSRFEPASSIRRLADARGTFYGSSPQRVNTEAAS"
     gene            3383..5101
                     /locus_tag="VF958_00675"
     CDS             3383..5101
                     /locus_tag="VF958_00675"
                     /inference="COORDINATES: protein motif:HMM:NF012656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEX9147648.1"
                     /translation="MSATPEAIPEALSSPIKALFAGQIVEQALFPYPQPSADERETVD
                     AFIGAFREFARDRIDSARIDREKTIPADVRRGLADLGIFGTSVPEEYGGYSFSSSAYC
                     RVTEEIGRIDGSLSILVGGHQSIGLKALLLYGTPEQKSRWLPPLAAGEKLAAFALTEP
                     EAGSDAASIRTTARYDPTKDEFVLDGSKHWISNGGIADFFTVFAKDVALDVKDEHRRI
                     TAFVVTRDLPGLEIGPEEKKLGLKGSSTVPLLFREVRVPASNVIGPRGSGFKVAVEVL
                     NTGRTALGAGCLGGCKAMIRESVLHATQRRQFGTRIADFEMIRGKFARMVIDTYALEG
                     AACKLFGTESVWRTINDALQIAGGNGFMEEYPYERALRDSRVNMIFEGTNEILRVLVA
                     LSGLRDIGSDLEEVARALKTPLSNLGILSEYVGRKIRGYVAPEKLESVVPELAGEADV
                     VTRYTRFFATAAETFLGRFGKNVLEREYQQERLANVAVDLYASLAVLSRASSSVRSAG
                     AEKAAGEIRVARTFVQSAKYRIVGELKEMEKNRDRERASLRDALRSEIAEGACSGGGY
                     RFDFWG"
     gene            complement(5037..>5517)
                     /locus_tag="VF958_00680"
     CDS             complement(5037..>5517)
                     /locus_tag="VF958_00680"
                     /inference="COORDINATES: protein motif:HMM:NF020132.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HEX9147649.1"
                     /translation="AFVREVLPLCDLVTPNLDEAAALSGLPIRTDADLREAARRICRL
                     GARAVLVTGGHGAGPRIQDLLWDGRRFRAFEHVRIDTRALHGTGCVLSAAVAANLALG
                     HPLEEAVARSIRYVRAALRRGVFPGQGAGSPGHPLALTPRSRSGSRPPSMRPRRFHS"
BASE COUNT          888 a         1841 c         1933 g          855 t
ORIGIN      
        1 acgcttctga aggacgttcc cgcacaggag ctcgcacgga tcgtcactac cgagcttctc
       61 gcgaggaccg gattcgatcc ggcggagctc gatcacgtga tcttcgggaa catcgcgcag
      121 cctccggacg ccgtgaatct ctcgcgcggg gcggcgctgc tcgcgggagt cccgcgccgc
      181 gtcccggcct tcacggtgaa ccggctgtgc ggctccggac tccagtcgat cgccgacgcg
      241 tggtaccgga tctcttccgg cgacgctgac gcaatcatcg ccggaggcgt cgagtccatg
      301 tcgaacatcc cgtttctcta ctcgcgcgag agccaggaga tcttcacgga ggtattcacg
      361 gcgaaggacc tcggcaagcg cgttgccgcg gccagccggt ttcgcccaaa gcacttcaag
      421 cccgagatcg gactccagca gggactgacg gacgcggtgt gcggcctgaa catgggggag
      481 accgccgagg tcctcgcgaa agagcaccac atctctcgcg aggagcagga cgcgttcgcg
      541 ctcaagtctc accgacgcgt cacgctcgcc cgctcgaagc tcgccgagga gatcgtgccg
      601 gtgcccattc ctccgaagta ctccgacatg gcgaccgccg acaacggcgt gcgcgagaac
      661 cagacgatgg aagcgctcgg gaagctccgc ccgtacttcg atccgaagtt cggcacggtc
      721 acggcgggca actcgtccca gatcaccgac gggggagccg cgaccctggt gatgagcgcc
      781 gaggcggcgt cggcccacgg ctaccggccg ctcggcaagg tgcggggctt cgcgttcgtg
      841 ggcgtcgacc cggagcggat ggggctcggt ccggccgcgg caacgcccct cgccttgaaa
      901 cgcgccggag tgtcgtgggg ggacatcggt ctcgtcgaga tgaacgaggc gttcgcggcg
      961 caatgtctcg cggtcgcgcg cgtctttccg tcgcgcgagt ggcacgagcg ttacgggacg
     1021 ggcgggccga tcggcgagat cgactgggag aggacgaacg tcaatggcgg cgcgatcgcg
     1081 ctcgggcatc cggtcgggtc atcggccaac cggctggtga cgacgctgct gaaggagatg
     1141 cgccggcgcg acgttccgct cggcctcgcg acgatgtgca tcgggggcgg gcagggcgga
     1201 tccgtggtcc tcgaggcgat gtgagcgcct tcggcctcga cgtcggcccc gacggactcg
     1261 gtctcctgac gttcgacctg cccggcgaga aggtcaacaa gttctctcgg gaagtcttcg
     1321 tcgagctttc cgaggtgctc gtccgtctct cgcaggatcc ccgggtccgg gcgctcctcg
     1381 tcgtcagcgg caaaccggac gttttcatcg ccggcgccga cgtcaaggag ttcacgcagg
     1441 tcaagccgga ggatgcgtcg gccggatccg cccgcggtca ggcgctcttc gagcagctcg
     1501 cgcgccttcc gtttccgacg gtcgccgcca tcaacggggt gtgcctgggg ggcggcaccg
     1561 agttcgccct ggcgtgcgac tggcgcgtga tgtccgacgc tgcctccgcc cgcatcgggt
     1621 tcccggaggt gcggctcggg atcttcccgg cgtggggtgg atgcgtccgc ctgccgcggc
     1681 tcgtcggtct cgcaaacgcg ctcgacctga tcctgacgag caaacagatc gacgcgcggc
     1741 gcgcgaaacg gatcggcctc gtcgacgagg cggtgcccgc cgcgatcttc gacgacttcg
     1801 cgcggcggtt cgcgcgttcc aagatcggcg cgggcaaacg cgctccgcga ccgacccgtc
     1861 tctcgtcggg agagcgagct ctcgaggcaa cctccatcgg ccggcggctc atcttcaaga
     1921 aggcccgaca gaacgttatc gagcagacgg gcggacacta tcccgcgccc ctcgaagccc
     1981 tggcagtcgt caccgagacc ttcggcatgc ctgtcgaggc cgcgctcgag gtcgagagcc
     2041 gccggatcgc caacctgttt ggaggcgagg tccaacgcaa cctcctcgac atcttctttg
     2101 caaccgaaca ggtgaaaaga gagacgggtg tcgacgatcc gggcgtccgg ccccgcggcg
     2161 tgacccacgt gggcatcctc ggcgcgggcg tcatgggcgg cggaatcgca cagctcgccg
     2221 cggaccgcgg gcttccggcg cgcatgaaag acatcgagcc gaaagccctc gcgcacggct
     2281 acgccgccgc ggcgtcggtc tggaaagagg ccctcggccg ccaccggctg acgccgcgcg
     2341 agatgacggt gcgtatggcg cgactctccg gcactctcga ctactcgggc ttcgcgcagt
     2401 gcgaggtgac gatcgaagcg gtcgtcgaga agctcgcggt caagcaggcc gtcctcgccg
     2461 agtgggagca cgcggtcccc gagaccgcga tcttcggctc caacacgtcg acgatcccga
     2521 tcggagagat cggcgcgtcg gcgaggcacc cggagcgggt cgcggggatg catttcttca
     2581 accccgtgca ccgcatgccg ctcgtcgagg tgatccgggg cgggaaatca tcggacgagg
     2641 cggttgcgac gattttcgcc ctcgcgaaga agctcggcaa gacaccggtc gtcgtccgcg
     2701 acatgccggg atttctcgtc aaccggatcc tcgctccata cctgtccgag gcggtccggc
     2761 tcgtgcggga ggggagccgg atcgaggacg tcgaccgcac gatgaaggac ttcggcatgc
     2821 ccgtcgggcc gctggccctt ctcgacgacg tcggcctcga cgtcgccgtg aaggcgggcg
     2881 aagtgctgca ggcggctttc ccgcagcgga taaagtcggg tggcgacgaa gcgctcgtcg
     2941 cggaaggccg cctcggtcga aagagcggcg gaggttttta cgagtacgcc ggaggcaaga
     3001 gaggcgcgcc ctccgggaag gcgtacgaga tcctccgcgc gcagccctcg tcctcgccgc
     3061 gtttctccgc ggaggtcatc gagtcgcgtc tcgtcctgcc gatggtcaac gaggccgcgt
     3121 tctgtctcga ggacggagtc gtccccggtg ccgggaaact cgatcttgcg atgatcatgg
     3181 ggaccggctt cccacccttt cgggggggcc tgctgaagtg ggcggactcg ctcgggctgc
     3241 cgcgagtccg ctccctgctc gaggagctca ccgaacggtt tggctctcgt ttcgagcccg
     3301 cgagctcgat ccgccggctc gcggacgccc gcgggacctt ctacggctcc tctccccaac
     3361 gcgtcaatac ggaggcggcg tcatgagcgc aacaccggaa gcgatccccg aggctctttc
     3421 gtccccgatc aaggccctct tcgccgggca gatcgtcgag caggcgctgt tcccgtaccc
     3481 ccagccgtcc gccgacgaga gagagacggt cgacgccttc atcggcgcct tccgggagtt
     3541 cgcccgggat cggatcgaca gcgcccggat cgatcgcgag aagacgatcc cggccgacgt
     3601 ccggcgcggc ctcgcggatc tcgggatctt cgggacctcc gtgccggagg agtacggcgg
     3661 atattccttc tcgtcgtcgg cctactgccg cgtgaccgag gaaatcgggc ggatcgacgg
     3721 ctcgctcagc atcctcgtcg gcggccacca gtccatcgga ctgaaggccc tcctccttta
     3781 cggaacgccg gagcagaaga gccggtggct cccgccgctc gccgcggggg agaaactcgc
     3841 cgccttcgcg ctgacggagc ccgaggccgg ctccgacgcc gcgtccatcc ggacgacggc
     3901 gcggtacgac ccgacgaagg acgagttcgt tctggacggc tcgaaacact ggatctcgaa
     3961 cggcgggatc gcggacttct tcacggtatt cgcgaaggac gtggccctcg acgtgaaaga
     4021 cgagcatcgg cggatcacgg cgttcgtcgt cacccgcgat ctgccgggac tcgagatcgg
     4081 accggaagag aagaagctcg ggctcaaggg ttcgtcgacc gtgccgcttc tcttccggga
     4141 ggttcgcgtc ccggcgtcga acgtcatcgg accgcggggc tcgggcttca aggttgcggt
     4201 ggaggtcctg aatacggggc gcacggcgct cggcgccggg tgcctgggcg gatgcaaggc
     4261 gatgatccgt gaatcggtgc tgcacgccac gcagcgccgg cagttcggaa cgaggatcgc
     4321 ggacttcgag atgatccgcg gcaagttcgc tcgaatggtg atcgacacct atgcgctcga
     4381 gggtgctgcc tgcaagctct tcgggaccga gtcggtgtgg agaacgatca acgacgctct
     4441 ccagatcgcg ggcggcaacg gtttcatgga ggagtacccg tacgagcggg cgctgcgcga
     4501 ctcccgcgtc aacatgatct tcgaaggcac gaacgagatc ctgcgggtcc tcgtcgcgct
     4561 ctcgggcctc cgcgacatcg gctcggacct cgaagaggtc gcccgcgcct tgaagacgcc
     4621 gctttcaaac ctcggaatcc tctccgagta cgtcggccgc aagattcgtg gctacgtcgc
     4681 gcccgagaaa ctggagtcgg tggtcccgga actcgccgga gaggccgacg tcgtcacccg
     4741 ctacacgcga ttcttcgcca cggcggcgga gacgttcctc gggcgcttcg gcaagaacgt
     4801 tctcgagcgg gagtaccagc aggagcgcct cgcgaacgtc gccgtcgacc tctacgcgtc
     4861 actcgccgtc ctctcacgcg cgagctcatc ggtgcggagc gcgggcgccg aaaaggccgc
     4921 cggggagatc cgtgtggccc ggacattcgt ccagagcgcg aagtatcgaa tcgtgggcga
     4981 gctgaaggag atggagaaga accgcgaccg ggagcgcgcg tcccttcgcg atgcgctcag
     5041 gagtgaaatc gccgagggcg catgctcggg gggcggctac cgcttcgact tctgggggtg
     5101 agcgcgaggg gatgaccggg acttccggcg ccctgcccgg gaaagacgcc gcggcgcagg
     5161 gcggcgcgca cgtagcggat cgagcgagcg accgcctctt ccagcggatg cccgagtgcg
     5221 aggttcgccg cgacggccgc ggaaagaacg cagcccgtgc cgtgcagcgc ccgggtgtcg
     5281 atccgcacgt gctcgaaggc gcggaaccgg cggccatccc acagaaggtc ctggatgcgc
     5341 gggccggcgc cgtgcccccc cgtcacgagc accgcacgcg cgccgaggcg gcagatgcgg
     5401 cgcgccgcct cccgaaggtc ggcgtcggtg cggatcggca ggccggacag cgcggcagcc
     5461 tcgtcgaggt tgggcgtgac gaggtcgcag agaggcagaa cctcgcgcac gaaggcg
//
LOCUS       DASWAV010000034         3180 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U2665
            k141_121789, whole genome shotgun sequence.
ACCESSION   DASWAV010000034 DASWAV010000000
VERSION     DASWAV010000034.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960316
            Sequence Read Archive: SRR10260007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 3180)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3180)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:47:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,929
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3180
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_121789"
                     /isolate="SMAG_U2665"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.36444 N 132.31889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..415
                     /locus_tag="VF958_00685"
     CDS             <1..415
                     /locus_tag="VF958_00685"
                     /inference="COORDINATES: protein motif:HMM:NF012899.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HEX9147650.1"
                     /translation="EGFLPVVASLGVGSDGHAYNVNADTAAAALAVAVGADKLVLLTD
                     VAGIYREIAGGRELLSELSPDEARLLVTSGAVTRGMIPKVSAALGAVEGGVPSAHIVG
                     AETPHSLLLELFTRSGIGTMIHAVAPEPMALASPA"
     gene            412..1662
                     /locus_tag="VF958_00690"
     CDS             412..1662
                     /locus_tag="VF958_00690"
                     /EC_number="2.6.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011393770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0006525 - arginine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylornithine transaminase"
                     /protein_id="HEX9147651.1"
                     /translation="MTKPSPFTDSASVAREADRVLFRNYKRAPVAFSHGRGVRLFDLE
                     GREYLDFIGGIAVSSLGHAHPALVAAIAGQAARYVHVSNLYQIPEQVAAAERLVAASG
                     LPRAFFCNSGAEANEAAIKLARKRAKSAKGPDCHEILVTKNSFHGRTLATLAATGNPR
                     YQQGFEPLPPGFRFVPYDDAEAMRSAVTDATCAILVEPVQGEGGILVPSPGYLALLRE
                     ICDENHLLLILDEVQTGIGRTGIMFAYQRSGVRPDLVTVAKGVAGGVPAGVLLATEEA
                     AAHFVPGDHGSTFGGNALASAAIVAVLDAIEREGLVENAAAMGARLVAGLEELRRKHP
                     VLLEVRGAGLLVGADLAIEAAPVVEACLAKGLLVNAVRPKTLRFAPPLVVSAADVDRA
                     LEIVNEVLAAVAVPTPAAAEKEIA"
     gene            1665..2906
                     /locus_tag="VF958_00695"
     CDS             1665..2906
                     /locus_tag="VF958_00695"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012642393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004055 - argininosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HEX9147652.1"
                     /translation="MKKVALAYSGGLDTSVIIPWLREKYDCQVVAVVADVGQLDDFAA
                     VERKAIASGASECRVVDVRDEFIRNYAFPALRAGAIYEGRYLLGTALARPLIARVQVE
                     VARETGCDALAHGCTGKGNDQVRFELTYRALAPELSVIAPWREWTLKSREDEIAYAAR
                     CGIPVPVTAAKPYSIDQNLWHTSYEGGVLEDPATPPPDDMFQLTVDPAEAPDEPAFVE
                     IEFESGIPVALNGARRQPADLVASLNALAGAHGVGRIDLVENRLVGMKSRGVYETPAG
                     TVLMAALRDLESLTLDRETHHFRETISPRYAELVYYGLWHSPLRNAFDAFLESSHRWV
                     SGTVRLKLFKGSCLAISRSSPYSLYRQDLATFGEDGVYDQKDATGFNNLWALPTRVVA
                     AVRRETESEAAAPAHLSVVRE"
     gene            2914..>3180
                     /locus_tag="VF958_00700"
     CDS             2914..>3180
                     /locus_tag="VF958_00700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HEX9147653.1"
                     /translation="MRTPLPQKLWGGRFEGDLDPEIQRFTTSFPVDRRLVRHDLVGSL
                     AHARMLRETGVLSAEDGAAILSGLSSMLWDVEEGRLVVEGDEEDV"
BASE COUNT          466 a         1148 c         1069 g          497 t
ORIGIN      
        1 ggagggcttc ctcccggtcg tcgcctcgct cggagtcggc tcggacggcc acgcctacaa
       61 cgtcaacgcc gacacggcgg cggcggccct cgcggtggcg gtcggcgcgg acaagctcgt
      121 tctcctgacg gacgtcgcgg ggatctaccg cgagatcgcg ggggggcggg aactcctctc
      181 ggagctctcc cccgacgaag cccggctcct cgtgacgtcg ggcgccgtga cccgcggaat
      241 gatcccgaaa gtctcggccg ccctcggtgc ggtcgagggg ggcgtgcctt ccgcccacat
      301 cgtcggcgcg gagacgcccc actctctcct cctcgaactg ttcactcgtt cggggatcgg
      361 cacgatgatc cacgccgtcg ctccggagcc gatggccctc gcctccccgg catgacgaaa
      421 ccctcgccgt tcaccgactc cgcgtccgtc gcccgtgagg ccgaccgcgt gctcttccgc
      481 aactacaagc gcgcgccggt cgcgttctcg cacgggcgcg gcgtccggct cttcgacctc
      541 gaggggcgcg aatacctcga cttcatcggc ggaatcgccg tgtcgagcct gggtcacgcg
      601 catccggcgc tcgtcgccgc gatcgccggg caggccgcgc ggtacgtgca cgtctcgaac
      661 ctctaccaga ttcccgaaca ggtcgccgcg gccgagcggc tcgtcgcggc gagcggcctc
      721 ccgcgggcgt tcttctgcaa cagcggcgcg gaggcgaacg aggcggcgat caagctcgcg
      781 cgcaagcgcg cgaagtccgc gaagggcccg gactgtcacg agattctcgt gacgaaaaac
      841 tccttccacg ggcgaacgct cgcgactctc gccgcgaccg gcaacccgcg ataccagcag
      901 ggattcgagc cgctgccgcc cggattccgt ttcgtgccct acgacgacgc ggaggcaatg
      961 cgaagcgcgg tgaccgatgc gacgtgtgca atcctggtcg agccggtgca gggcgagggc
     1021 ggaatcctcg tcccttcgcc cggctacctc gccctcctcc gcgagatctg cgacgagaac
     1081 catcttctgc tgatcctgga cgaggtccag accgggatcg ggcgcaccgg aatcatgttt
     1141 gcctaccagc ggtcgggtgt ccggcccgac ctcgtcacgg tcgccaaggg tgtcgccgga
     1201 ggcgtgccgg ccggggtcct cctcgcgacg gaggaagccg ccgcgcactt cgtccccgga
     1261 gaccacggtt cgacgttcgg cggaaacgcc ctcgcttccg cggccatcgt cgccgtgctc
     1321 gacgcgatcg agcgcgaagg cctggtggag aacgcggctg ccatgggcgc gcggctcgtc
     1381 gccgggctcg aagagctgcg gcggaaacat cccgttctgc tcgaggtccg tggcgcgggg
     1441 ctgctcgtcg gcgccgatct cgcgatcgag gcggcgcccg tcgtcgaggc gtgccttgca
     1501 aaaggtctcc tcgtcaacgc cgtgcgcccg aagactctcc gattcgcgcc gccgctcgtc
     1561 gtttccgcgg ccgacgtgga ccgcgcgctc gaaatcgtga acgaagttct cgcggccgtc
     1621 gcggttccga ctcccgccgc cgccgaaaag gagatcgctt gaaagtgaag aaggtcgccc
     1681 tcgcctactc cgggggcctc gacacctccg tgatcattcc gtggctgcgg gagaaatacg
     1741 actgccaggt cgtggccgtt gtggccgacg taggccagct cgacgatttc gcggccgtcg
     1801 agcggaaagc gatcgcctcc ggcgcgtccg agtgccgcgt cgtggacgtg cgcgacgagt
     1861 tcatccgcaa ctacgcattt cccgcgctgc gcgccggcgc catctacgag gggaggtatc
     1921 tcctcggaac ggcgctcgcg cggcctctca tcgcccgggt ccaggtcgag gtcgccagag
     1981 agaccggctg cgacgccctc gcgcacgggt gcaccggtaa gggaaacgac caggtgcgat
     2041 tcgagctcac ctatcgcgcc ctcgcaccgg agctctcggt catcgccccg tggcgcgagt
     2101 ggacgctcaa atcgcgcgag gacgagatcg cctacgccgc caggtgcggc atcccggtgc
     2161 cggtcaccgc cgcgaagcct tactcgatcg accagaacct gtggcacacc tcctacgagg
     2221 ggggcgtcct cgaggacccg gccacgccgc ctcccgacga catgttccag ttgaccgtcg
     2281 atcctgccga ggcgccggac gagccggcgt tcgtcgagat cgagttcgag agcggcatcc
     2341 cggtcgcgtt gaacggcgcg cgccgccagc ccgcggacct cgtcgcgtcg ctgaacgctc
     2401 tcgccggcgc gcacggcgtc gggcggatcg atctcgtcga gaaccggctc gtgggcatga
     2461 agagccgggg cgtctacgag actccggcgg gaaccgtcct catggcggct cttcgcgacc
     2521 tcgaatccct gacgctcgac cgcgagacgc accacttccg cgagacgatc tccccgcgct
     2581 acgccgagct cgtctactac ggcctctggc actctcccct tcgcaacgcc ttcgatgcct
     2641 tcctcgagtc gtcccaccgc tgggtctccg gcacggtgcg gctgaaactc ttcaagggct
     2701 cctgtctcgc gatctcgcgg tcctcgccct actcgctcta ccggcaggac ctcgcgacgt
     2761 tcggcgaaga cggcgtctac gaccagaagg acgcgacggg cttcaacaac ctctgggcgc
     2821 tgccgacgcg ggtggtcgcg gccgtccgca gggagacgga gtccgaggcg gcggcaccag
     2881 cgcacctctc cgtcgtgcgc gagtgagcgc ggcgtgagaa cgcctctccc gcagaagctc
     2941 tggggcggcc gcttcgaggg ggacctcgac ccggagatcc agcgattcac gacgtccttt
     3001 cccgtcgatc gccggctggt gcgccacgac ctcgtcggca gcctcgcgca cgcgaggatg
     3061 ctccgggaga ccggcgtcct ctcggcggaa gacggcgcgg cgatcctgtc gggcctgtcc
     3121 tcgatgctct gggacgtcga ggagggacgc ctcgtggtcg aaggggacga ggaggacgtc
//
LOCUS       DASWAV010000035         9281 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U2665
            k141_561728, whole genome shotgun sequence.
ACCESSION   DASWAV010000035 DASWAV010000000
VERSION     DASWAV010000035.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960316
            Sequence Read Archive: SRR10260007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 9281)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9281)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:47:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,929
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9281
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_561728"
                     /isolate="SMAG_U2665"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.36444 N 132.31889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1225
                     /locus_tag="VF958_00705"
     CDS             <1..1225
                     /locus_tag="VF958_00705"
                     /inference="COORDINATES: protein motif:HMM:TIGR01420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="PilT/PilU family type 4a pilus ATPase"
                     /protein_id="HEX9147654.1"
                     /translation="DIGYGLAGVARFRANVYVQRGSLAAVFRRIPFKLPSIEELNLPE
                     VLETFVHLPAGLVLITGPTGSGKSTTLAGILKAVIEQRQVHIVTIEDPIEYLFADGKA
                     AVSQRELETDTPSFAEALKNMFREDPDVIMVGEMRDWQTMQTTLTAAETGHLVFSTLH
                     TNSASQSIDRIIDACPSNLQKQIRSQVALVLRAIVSMKLIEKADGTGLLPVVEILVNS
                     PKIAKHIEVGETKEIQEEMETSVAYYRMQSMNQSLIALLVNGQIDYNRAMELSTDPED
                     LSLKLRKLFPQIEERGQGDEMPSVNDFSHISRLMETKNLYEQQEQNWRLRLAEKDEEV
                     AHQKAEADNLRHATEERHKAVGELETEIAKLRAENERLTKEGQQKIAQLNERIKELNQ
                     RVLIAEGGSRMTARR"
     gene            complement(1226..1591)
                     /locus_tag="VF958_00710"
     CDS             complement(1226..1591)
                     /locus_tag="VF958_00710"
                     /inference="COORDINATES: protein motif:HMM:NF037970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HEX9147655.1"
                     /translation="MKRARIAAALWGGFLFLLTSWPNPPAIPAGGFPLDKLTHFVLYG
                     VEAFLIDRAIPRKGRPGLALGRVLAITGAMALWGMLDEAHQEWIPGRRMDLEDLLADV
                     SGAAVGAVAAGVLSRVSKR"
     gene            complement(1606..2211)
                     /gene="rplQ"
                     /locus_tag="VF958_00715"
     CDS             complement(1606..2211)
                     /gene="rplQ"
                     /locus_tag="VF958_00715"
                     /inference="COORDINATES: protein motif:HMM:TIGR00059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005762 - mitochondrial large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HEX9147656.1"
                     /translation="MQHNRAGRKLRRTTSHRLAMFSNQLASLMTHERIQTTVSKAKEL
                     RPLAERLITAAKNDDVAARRRVYRWIPDHLTVKKVFESIAPRFVDRPGGYTRILKLGA
                     RRGDAAEAAILEFVDYKFQAKEKGPRKESLSDRARRMSGGRVETVRKQKAEAEGEEGA
                     AEEAPRKATRKAKGSRSEGAKAAKPAPKKGPSRTGPSKRGA"
     gene            complement(2214..3194)
                     /locus_tag="VF958_00720"
     CDS             complement(2214..3194)
                     /locus_tag="VF958_00720"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020719819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HEX9147657.1"
                     /translation="MTLWKGFQKPKRLEVEAETLTATYGKFYAQPFERGFGTTIGNSL
                     RRVLLSSIEGAAITAVKIEGVLHEFSSLPSVVEDVTDIILNLKQIPIKMHTTEEKMLT
                     LDVTRKGQVTASDLQGDSTIEILEPDAPIATLSENGKLQMEMRVSMGRGYVSADKNFD
                     EDMGIGYIPIDSVHSPVRRVNYHVEAARIGQATDYDRLVLEVWTNGTVSPADAVGLAA
                     TLMRDHLTIFVNAEVETALEGVGEGELPENLAEYLNKSVDELELSVRSANCLKNAGIR
                     TIRDLVQKSERDMLETKNFGRKSLNEIKDILRPMGLSLGMELEAAEARSE"
     gene            complement(3255..3881)
                     /gene="rpsD"
                     /locus_tag="VF958_00725"
     CDS             complement(3255..3881)
                     /gene="rpsD"
                     /locus_tag="VF958_00725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015838539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HEX9147658.1"
                     /translation="MARYTESVCRLCRREGMKLFLKGDRCFKDKCAIERRNYPPGQHG
                     RRRSKLLGYGIQLREKQKVKRIYGLLERPFRLTFQRAERRKGITGANLLQELERRLDN
                     VAFSLGFAASRAQARQLVCHGHVTVNGRKVTIPSFRVSKGNVVAIKAKSRSNEQIKAS
                     VETARARGVPGWLDLQPESFSGTVLELPSREEIKLPIQEQLIVELYSK"
     gene            complement(3993..4439)
                     /gene="rpsK"
                     /locus_tag="VF958_00730"
     CDS             complement(3993..4439)
                     /gene="rpsK"
                     /locus_tag="VF958_00730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003925928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HEX9147659.1"
                     /translation="MAEPKTEAVSAAPEKPAGGKRTKKPRGGKKEKKNIPHAIATVQA
                     SFNNTIIAIADQEGNILAWSSAGRIGFKGSRKGTPFAAQLAAANCAEHAKENGVRSVD
                     VRVTGPGAGRESAIRALQANGIDIKSIKDTTPIPHNGCRPRKRRRV"
     gene            complement(4519..4902)
                     /gene="rpsM"
                     /locus_tag="VF958_00735"
     CDS             complement(4519..4902)
                     /gene="rpsM"
                     /locus_tag="VF958_00735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008633368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HEX9147660.1"
                     /translation="MARIAGVDLPLNKRVEIGLTYIYGIGRSKANVILEKADVRPNTQ
                     VKDLSEDEVVRIRKVVTDDVKVEGDLRRDISVDIKRLMDIGCYRGMRHRRNLPVRGQR
                     THTNARTRKGPRRQTIAGKKKATKK"
     gene            complement(4906..5019)
                     /gene="rpmJ"
                     /locus_tag="VF958_00740"
     CDS             complement(4906..5019)
                     /gene="rpmJ"
                     /locus_tag="VF958_00740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006232360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HEX9147661.1"
                     /translation="MKVRASVKRICAKCKIVRRNGVVRVICPIPKHKQRQG"
     gene            complement(5074..5292)
                     /gene="infA"
                     /locus_tag="VF958_00745"
     CDS             complement(5074..5292)
                     /gene="infA"
                     /locus_tag="VF958_00745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012611021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HEX9147662.1"
                     /translation="MTKEDAIEVMATVVEPLPNAMFKVELENKHQVLAHISGKMRKNF
                     IRILPGDKVLVELSPYDLTRGRIIYRYK"
     gene            complement(5289..6080)
                     /gene="map"
                     /locus_tag="VF958_00750"
     CDS             complement(5289..6080)
                     /gene="map"
                     /locus_tag="VF958_00750"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011345170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HEX9147663.1"
                     /translation="MITIRSADELARLEQASRVVLETLEVLEKAVEPGVTTEELDRIA
                     ASEIARRGARPAFLGYRGFPKTICTSVNDEVVHGIPGKRSLKDGDIIGIDCGAVVDGY
                     FGDAARTLPVGRIDSARERLLAVTREALHAGITAARPGGRVSDIGAAVEAVAMAHGYG
                     VVREFVGHGVGTALHEEPQIPNYGPAGRGSRLAPGMVLAIEPMFNLGTCEVSVDRDGW
                     TVRTHDRSASAHFENTIVIGAEGPVILGLGRVRSAADREPAAIPA"
     gene            complement(6077..6670)
                     /locus_tag="VF958_00755"
     CDS             complement(6077..6670)
                     /locus_tag="VF958_00755"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007514209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019205 - nucleobase-containing compound
                     kinase activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HEX9147664.1"
                     /translation="MRVIFLGPPGCGKGTQAKLLGERLGVPAVSTGDMLREAVRQGTP
                     LGLRAKSIMDAGELVPDDVVIGLIRERIGAPDARQGFLLDGFPRTVEQARALDRLLAG
                     NGVSLDTVLNLLVPETTLVERMLRRAVNEGRSDDRPETVAERLRVYREKTAPLVDHYR
                     ALGVLSDIEGSGEIAEIAGRIDHALAESARARRERRA"
     gene            complement(6678..8066)
                     /gene="secY"
                     /locus_tag="VF958_00760"
     CDS             complement(6678..8066)
                     /gene="secY"
                     /locus_tag="VF958_00760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005007692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HEX9147665.1"
                     /translation="MSALQSFANVFNVPDLRKRVVFTFLLLAVYRLGSHIPTPGVDPA
                     AIDAFFRSSAGSLFGFLDIFSGGALRRLSVFALGIMPYISASIILQLLTVVWPYLEKL
                     SKEGEMGRKKITQYTRYGTVLLSFIQASGIALWLEKQVAPTGARLVPHAGWGFRLMTI
                     LTLTTGTIFIMWLGEQITERGIGNGISLIIFAGIVVGLPRAIFDLFQQIRGGTINILT
                     LIGVLAFMIGIIALIVFVERAQRRIPVQYAKRVVGRRIYGGQNTYLPLRVNTGGVIPI
                     IFAISIIQLPSYIATLVGADWTKKVANSLGQGQPLYVVLYALGILFFCYFYTSIVFNP
                     TDTADNMRKYGGFIPGIRPGKKTADYIDTVLSRLTIIGALYLTFVALLPDFLSRGFRF
                     ASLPWFGARLEALLPRFFSEGFGFNFYFGGTSLLIVVGVAMDTVQQVESKLVEHHYTG
                     FLKKSRIRGRRS"
     gene            complement(8063..8524)
                     /gene="rplO"
                     /locus_tag="VF958_00765"
     CDS             complement(8063..8524)
                     /gene="rplO"
                     /locus_tag="VF958_00765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008287615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HEX9147666.1"
                     /translation="MARPRWTGVSDLRPAPGATRSRKRVGRGPGSGHGKTAGRGSKGQ
                     KSRSGYRHQRGFEGGQMPLHRRVPKRGFTNIFRIEYDVVNLSALEAFDAGESVTPDKL
                     AEKRLARGRRPVKILGGGELKKPLTVSAHKFSASARSRIEAAGGSCEVLNR"
     gene            complement(8740..9009)
                     /gene="rpmD"
                     /locus_tag="VF958_00770"
     CDS             complement(8740..9009)
                     /gene="rpmD"
                     /locus_tag="VF958_00770"
                     /inference="COORDINATES: protein motif:HMM:TIGR01308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HEX9147667.1"
                     /translation="MSESPTSSTAPAAGKRRARKPAASRPDGAHKGMLRLKLAKSGIA
                     TPKNQKATIRALGFRRVGEVVERPDTPEVRGRIRKIRHLVEIIGA"
     gene            complement(9006..>9281)
                     /locus_tag="VF958_00775"
     CDS             complement(9006..>9281)
                     /locus_tag="VF958_00775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015851083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9147668.1"
                     /translation="VLGRYGAGRVLLKPAAPGTGVIAGGPVRAVLESVGIQDILTKSL
                     RTTNPHNVIKATFDGLTRLKNEDEILKRRGFAGGAGARDAQRVEAEA"
BASE COUNT         1485 a         3147 c         2944 g         1705 t
ORIGIN      
        1 cgacatcggc tatggcctgg ccggcgttgc ccgtttccgc gccaacgtct acgtccagcg
       61 ggggtcgctc gccgcggtct ttcgccggat tccgttcaag ctcccgtcga tcgaggagct
      121 gaacctcccc gaagtcctcg aaacgttcgt ccacctcccg gccggcctcg tcctcatcac
      181 ggggccgacc ggctcgggca agtcgacgac gctcgccggc atcctgaaag cggtcatcga
      241 gcagcggcag gtccacatcg tcacgatcga ggacccgatc gagtacctct tcgccgacgg
      301 caaggcggcg gtctcacaga gggagctcga gaccgacact ccaagcttcg ccgaggcgct
      361 caagaacatg ttccgggaag accccgacgt catcatggtc ggcgaaatgc gggactggca
      421 gacgatgcag acgacgctga cggccgccga aaccggtcac ctcgtcttct cgacgctcca
      481 cacgaacagc gcgagccagt cgattgaccg gatcatcgac gcctgcccct cgaacctcca
      541 gaagcagatc cggtcacagg tcgcgctcgt cctgcgcgcg atcgtctcga tgaagctgat
      601 cgaaaaggcc gacgggaccg gcctcctccc ggtcgtcgag atcctcgtga actccccgaa
      661 gatcgccaag cacatcgagg tcggcgagac caaggagatc caggaggaga tggaaacgtc
      721 ggtcgcctac tatcgcatgc agtcgatgaa ccagtcgctc atcgcgctcc tcgtgaatgg
      781 ccagatcgac tacaaccgcg cgatggaact ctcgaccgat cccgaggatc tgtccttgaa
      841 gctccgcaag ctcttccctc agatcgaaga gcgcgggcaa ggagacgaaa tgccgtccgt
      901 gaacgatttt tcgcacatct cgcgtctcat ggagacgaag aacctctacg agcagcagga
      961 gcaaaactgg cggctccgcc tggctgaaaa ggacgaagag gtcgcgcacc agaaggccga
     1021 ggccgacaat ctccgacacg cgacggagga gcggcacaag gcggtcggcg agctcgagac
     1081 ggagatcgcg aagctccggg ccgagaacga gcggctcacg aaggaaggtc agcagaagat
     1141 cgcgcagttg aacgagcgga tcaaggagct gaaccagcgt gtcttgatcg ccgagggcgg
     1201 cagccggatg accgcgagac ggtagctacc ttttcgatac tcgcgacagc acgcccgccg
     1261 caacggcccc gacggccgcg ccgctgacgt cggccagcag atcctctaaa tccattcgac
     1321 ggcccgggat ccactcctgg tgagcctcgt cgagcatccc ccacagtgcc atcgctccgg
     1381 tgatcgcgag cacgcgtccg agagcgagcc ccggccgacc cttccgcggg atcgcgcgat
     1441 cgatcaggaa cgcctcgacc ccgtagagga cgaagtgagt gagcttgtcg agcgggaaac
     1501 cgccggccgg gatcgccgga ggattcggcc aggacgtcag gagaaaaaga aagccgcccc
     1561 agagggcggc tgcgattcgt gcgcgtttca ctcgccggtc ggtgctcacg ccccgcgctt
     1621 ggacggtccc gtgcgactcg gtcccttctt cggagcgggc ttcgcggcct ttgcaccctc
     1681 ggagcggctg cccttcgcct tgcgggtggc cttcctcgga gcctcttccg ccgcgccctc
     1741 ctcgccctcc gcttccgcct tctgcttgcg gacggtctcg acccggccgc ccgacatccg
     1801 ccgagcgcgg tcggagagcg actccttcct cggtcccttt tccttcgcct ggaacttgta
     1861 gtcgacgaac tcgagaatgg cggcctcggc ggcgtcgccg cgccgagccc ccagcttcag
     1921 aatccgcgtg tatccgccgg gtcgatccac gaagcgcggt gcgatcgact cgaagacctt
     1981 cttgaccgtg aggtggtcgg gaatccagcg gtagacgcgg cggcgcgccg caacgtcgtc
     2041 gttcttggcg gccgtgatga gtcgctcggc cagcgggcgc agctccttcg ccttcgagac
     2101 ggtcgtctgg atccgctcgt gtgtcatgag cgatgccagc tggttcgaga acattgccag
     2161 ccggtgcgac gtggtgcgcc ggagcttgcg tccggcccgg ttgtgctgca tggctactcg
     2221 cttctcgctt ccgccgcctc gagctccatt ccgagggaga gacccatggg gcggaggatg
     2281 tccttgattt cgttcagcga cttgcggccg aaattcttcg tctcgagcat gtcccgctcg
     2341 ctcttctgca cgaggtcccg gatggtccgg atgcccgcgt tcttgaggca gttggcgctg
     2401 cgaacggaga gctcgagctc gtcgaccgac ttgttgaggt actccgcgag gttctccggg
     2461 agttcaccct cccccacgcc ttcgagcgcc gtctcgacct cggcgttcac gaagatcgtc
     2521 aggtggtcgc gcatcagcgt cgccgcgagg ccgaccgcgt cggcgggcga gacggttccg
     2581 ttcgtccaga cctcgaggac gagccggtcg tagtccgtcg cctggccgat ccgggccgcc
     2641 tcgacgtggt agttcacgcg acgcaccggg gagtggacgg agtcgatcgg gatgtaaccg
     2701 atgcccatgt cctcgtcgaa gttcttgtcc gcggagacgt acccccggcc catcgagacc
     2761 cgcatctcca tctggagctt tccgttctcc gagagcgtcg cgatcggcgc gtcgggctcg
     2821 aggatctcga tcgtcgagtc tccctggagg tcggacgccg tcacctgacc cttgcgggtc
     2881 acgtcgaggg tcagcatctt ctcctcggtc gtgtgcatct tgatggggat ctgcttcagg
     2941 ttgaggatga tgtcggtcac gtcctcgact acgctcggca gcgacgagaa ctcgtgcagg
     3001 accccctcga tcttcaccgc ggtgatcgcc gcaccttcga tcgaggagag cagcacccga
     3061 cgcagagaat tgccgatcgt ggtgccgaag cccctctcga acggctgcgc ataaaacttc
     3121 ccgtaggtgg ccgtgagcgt ctccgcctcg acctcgagtc gcttcggttt ctgaaatccc
     3181 ttccacaaag tcatgacttt ttctcctgag ggagggacca ggtctgacct gtgcccacac
     3241 ccgacctcac acccttactt cgaatagagc tcgacgatca gctgctcctg gatgggcagc
     3301 ttgatctctt cccggctcgg cagctccagc accgttccac tgaaggactc cggctggaga
     3361 tcgagccatc ccggcacgcc gcgcgcgcgc gcggtctcaa ccgaggcctt gatctgttcg
     3421 ttggaccggc tcttcgcctt gatcgcgacc acgttgccct tggacacacg gaaggacggg
     3481 atcgtgacct tcctcccgtt gacggtgacg tggccgtggc acacgagctg gcgcgcctgg
     3541 gcgcgcgacg ccgcgaaacc gagcgagaac gcgacgttgt cgagtcttct ctcgagctcc
     3601 tggagcagat tcgctcccgt gatccccttg cgcctttcag cgcgctggaa cgtcaggcgg
     3661 aacgggcgct cgagcagccc gtagatgcgc ttgaccttct gcttttcgcg cagctggatg
     3721 ccgtaaccga gcagcttgct ccgccgccgg ccgtgctggc cgggcgggta gttgcggcgc
     3781 tcgatggcgc acttgtcctt gaagcagcgg tcccctttca ggaacagctt catcccctcg
     3841 cgccggcaaa gccggcaaac cgactcggtg taacgcgcca agacggaacc tcctcgatcc
     3901 ctcttctatt tttcgtcctt cctcccggct gggaccgagg agccggttta cggacgagct
     3961 ggcagccgcg tgctgtcagc tagcagcttt ccttagacgc gccgccgctt tctcggccgg
     4021 cagccgttgt gggggatcgg cgtcgtgtcc ttgatggact tgatgtcgat cccgttcgcc
     4081 tgaagggccc gaatggccga ttcccgcccg gcgccggggc ccgtgacccg gacgtcgacc
     4141 gatcgcacgc cgttttcctt cgcgtgttcc gcgcagttgg ccgcggcgag ctgcgccgcg
     4201 aacggagttc ccttgcgcga tcccttgaac ccgatccgcc ccgcggagga ccacgccagg
     4261 atgttcccct cctggtcggc gatcgcgatg atcgtgttgt tgaacgaggc ctgcacggtc
     4321 gcgatcgcgt gcgggatgtt cttcttttcc ttcttcccgc cccgcggctt cttcgtgcgc
     4381 ttcccaccgg cgggtttttc gggagccgcg gacaccgcct cggtttttgg ttcggccatg
     4441 gttcctctct aaagtcgcgg ggacaggtcc tacggacctg cacccgccgc agttctctgc
     4501 gatcgaatga gcaaggcttt atttcttggt cgccttcttc ttgccggcga tcgtctggcg
     4561 gcgaggccct ttacgggtcc gcgcgttcgt gtgcgtgcgt tgtccgcgca ccggaaggtt
     4621 gcggcggtgt cgcattccgc gatagcagcc gatgtccatc aggcgcttga tgtccacgga
     4681 gatgtcgcgg cgcaggtctc cctcgacctt gacgtcgtcg gtgaccacct tgcgaatccg
     4741 gacgacctcg tcctcggaga ggtccttcac ctgggtgttc ggccgcacgt ccgccttctc
     4801 gaggatgacg ttcgccttgg accggccgat gccgtagatg tacgtcaggc cgatctcgac
     4861 ccgcttgttc agaggcaaat caacgccggc gattcgtgcc atgtcttacc cctgtctctg
     4921 cttgtgcttc gggatcgggc agatgacccg aaccacaccg tttctccgta cgatcttgca
     4981 tttcgcgcag attcgtttga ccgacgcccg gactttcacg accgactccc taatgttgtg
     5041 gcgggtgcag gtccgccgga cctgtccccg cgcctactta tatcgataga tgatccgccc
     5101 gcgcgtcagg tcgtacgggg agagctccac gaggaccttg tcccccggaa ggatgcggat
     5161 gaagttcttc ctcatcttcc cggagatgtg cgcgagcacc tgatgtttgt tctccaactc
     5221 caccttgaac atcgcgtttg gcaacggctc gacgaccgtc gccatcacct cgattgcgtc
     5281 ttccttcgtc atgccgggat cgccgcgggt tcccggtcgg cggcgctccg aacgcggccg
     5341 aggccgagga tcaccggccc ttcggcgccg atcacgatgg tgttctcgaa gtgcgcggac
     5401 gcactccgat cgtgcgtccg gacggtccac ccgtcgcgat cgacggagac ctcgcacgtc
     5461 ccgaggttga acatcggctc gatcgccagc accatcccgg gagcgagccg gctccccctt
     5521 cccgcgggtc catagttcgg gatctgcggc tcttcgtgga gagcggttcc gacaccgtgg
     5581 ccgacgaact cgcgcacgac cccgtagccg tgcgccatcg cgaccgcctc gaccgcggcg
     5641 ccgatgtcgg agacccttcc acccgggcgc gcggccgtaa tgccggcgtg caaagcctcg
     5701 cgcgtgaccg cgagcagcct ttcgcgggcc gaatcgatcc ggccgacggg aagcgtccgc
     5761 gcggcgtcgc cgaagtaccc gtcgacgacc gccccgcagt cgattccgat gatgtcgccg
     5821 tctttcaaag atcgcttccc cgggattccg tggacgacct cgtcgttcac cgacgtgcag
     5881 atcgtcttcg ggaacccccg atatccgaga aacgccggcc gcgctccgcg gcgggcgatc
     5941 tcgctggccg cgatccggtc cagctcctcc gtcgtgaccc ccggctcgac cgccttttcg
     6001 agcacttcga gggtctccag cacgacccgc gaggcctgct cgagccgggc gagctcgtct
     6061 gcactccgga tcgtgatcat gcccggcgtt cccgacgcgc cctggccgac tccgcgaggg
     6121 cgtgatcgat gcgtcccgcg atctcggcga tctcgcccga tccttcgatg tcggagagga
     6181 ccccgagcgc ccggtagtga tccaccagcg gcgccgtttt ctcccggtaa acccggagcc
     6241 gctccgcgac cgtctccggc cggtcgtccg aacggccttc gttcacggca cggcgcagca
     6301 tccgctcgac gagggtcgtt tcgggcacga ggaggttcaa aacagtgtcc agcgaaactc
     6361 catttccggc gagcaggcgg tcgagggcgc gcgcctgctc gaccgtgcgc gggaacccgt
     6421 cgaggaggaa cccctggcgg gcgtcgggag ccccgattcg ctcccggatc aggccgatca
     6481 cgacgtcgtc gggtacgagc tcgcccgcgt ccatgatcga tttcgcccgc agcccgagcg
     6541 gggtgccctg gcggaccgcc tcgcgcagca tgtcgccggt cgagacggcg ggcaccccca
     6601 gcctctcccc gaggagcttc gcctgggtgc ccttgccgca tcccggcggc ccgagaaaga
     6661 tgactcgcat cgtgtgccta gctgcggcgg ccgcgaatcc ggctcttctt caagaacccg
     6721 gtgtagtggt gctcgaccag cttcgattcg acctgctgaa cggtgtccat cgcgacgccg
     6781 acgacgatca gaagcgacgt cccgccgaag tagaagttga agccgaaccc ctcgctgaag
     6841 aaccgcggca ggagcgcctc gagcctggcg ccgaaccacg ggaggctcgc gaagcggaaa
     6901 ccgcgcgaga ggaagtccgg gaggagcgcc acgaaggtga ggtacagggc gccgatgatc
     6961 gtgaggcgcg agagcaccgt atcgatgtag tccgcggtct tcttcccggg ccggatgccc
     7021 ggaatgaagc caccgtactt gcgcatgttg tcggcggtgt cggtcgggtt gaagacgatc
     7081 gacgtgtaga agtagcagaa gaagaggatg ccgagggcgt agagaacgac gtaaagaggc
     7141 tgcccctgtc cgagcgagtt cgccaccttt ttcgtccagt cggcgccgac gagggtcgcg
     7201 atgtagctcg gcagctggat gatcgagatc gcgaagatga tggggatcac gccgccggtg
     7261 ttgacgcgca gcgggagata cgtgttctgc ccgccgtaga tccgacggcc gacgacgcgc
     7321 ttggcgtatt gcacgggaat gcgcctttgc gcgcgttcga cgaagacaat gagcgcgatg
     7381 atcccgatca tgaacgcgag cacgccgatg agcgtcagga tgttgatcgt gccgccccgg
     7441 atctgctgga agagatcaaa gatcgcgcgc ggcagtccga cgacgatgcc ggcgaagatg
     7501 atgagagaga ttccgtttcc gatcccgcgc tcggtaatct gttctccgag ccacatgatg
     7561 aagatcgtgc cggtcgtcag cgtcaagatc gtcatgaggc ggaagcccca gcctgcgtgc
     7621 ggcacgaggc gcgcgccggt cggggccacc tgcttctcga gccagagagc gatgccggag
     7681 gcctggatga acgagaggag cacggtgccg tagcgggtgt actgcgtgat cttctttcgc
     7741 cccatctccc cttccttgga gagcttttcg agatagggcc agacgaccgt caggagctga
     7801 aggatgatcg acgccgaaat gtagggcatg atcccgagcg cgaacaccga gagcctccgc
     7861 agcgctcctc ccgagaagat gtcgaggaaa ccgaacagcg atcccgccga ggatcggaag
     7921 aacgcgtcga tcgccgccgg gtcgacgccc ggcgtcggga tgtgcgaccc caggcgatac
     7981 accgcgagca gcaggaacgt gaacacgacc cgcttgcgca ggtcgggaac gttgaagacg
     8041 ttggcaaaac tctggagcgc gctcaacggt tgagaacctc gcaactgccg ccggccgcct
     8101 cgattcgaga tcgcgccgac gccgagaact tatgggcgga gacggtcagc ggcttcttca
     8161 gctcgccgcc gccgaggatc ttcacgggcc gacgtccccg ggcgagccgt ttctccgcga
     8221 gcttgtccgg ggtcacgctc tcacccgcgt cgaacgcttc gagcgccgag aggttcacga
     8281 cgtcgtactc gatgcggaag atgttcgtga acccccgttt cggcacgcga cggtggagcg
     8341 gcatctgccc tccctcgaat ccgcgctggt gccgataacc ggaccgcgac ttctgtccct
     8401 tgctgccgcg gcccgccgtc ttgccgtggc cgctaccggg cccccggccg acccgttttc
     8461 tcgagcgggt cgcgcccggt gcgggacgga ggtccgagac cccggtccag cggggccggg
     8521 caatctcttc acggccggac gccgcgcgcg cttctcccgg ggcagccgcc gccggccggg
     8581 caaccctctt tcgccgcttc tcggcaggcg cctcggctcg ggcggccttc ggcggggccg
     8641 ccgcctttgc cggcttcacg ggtttcgccg gctttttcgc cggagccttc ttcttcgttt
     8701 cttttttctc agccatcaga agttctcagt tcgtgagttt caggcgccga tgatttcgac
     8761 cagatgccgg atcttgcgga tccggccccg cacttccggg gtatccggcc gctcgacgac
     8821 ctcgccgacg cggcgaaacc cgagcgcgcg aatcgtcgcc ttctggttct tcggcgtcgc
     8881 gatcccgctc ttcgcgagtt ttaaacgaag catccccttg tgagcgccgt ccggcctgct
     8941 cgccgccggc ttgcgggcgc gccgcttccc cgcggcgggt gccgtggagc tcgtcggaga
     9001 ttcgctcatg cctcggcctc gaccctttgc gcgtcccgcg cgccggcccc gccggcaaag
     9061 ccgcggcgct tcaagatctc gtcctcgttc ttcaggcgcg tcaggccgtc gaacgtcgcc
     9121 ttgatgacgt tgtgcgggtt cgtcgtgcgg agggacttcg tcaggatgtc ctgaattccc
     9181 accgactcga ggaccgcccg caccggaccg ccggcgatga ccccggttcc gggcgcggcg
     9241 ggcttcagca gcacgcgccc cgcgccgtag cgtccgagaa c
//
LOCUS       DASWAV010000036         2471 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U2665
            k141_242331, whole genome shotgun sequence.
ACCESSION   DASWAV010000036 DASWAV010000000
VERSION     DASWAV010000036.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960316
            Sequence Read Archive: SRR10260007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2471)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2471)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:47:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,929
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2471
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_242331"
                     /isolate="SMAG_U2665"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.36444 N 132.31889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            137..1342
                     /locus_tag="VF958_00780"
     CDS             137..1342
                     /locus_tag="VF958_00780"
                     /inference="COORDINATES: protein motif:HMM:NF015368.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9147669.1"
                     /translation="MKRTVLLVLSSILIATCASQNTNKVANAAPTAASYVADSKTIPV
                     GVIPSAIVHDAVRIKDLELSIDYPAKDGPYPVIIFSPGYGSSNLTYVGTSSFWASHGY
                     VVMKPKHADAGALRPAENEMPGERPEQRREGRRDRRQQNADQPPQPRVCHPNPAEVWD
                     GQTPADWQNRVADIRLILDNLGKLVEQYPELKPRLDATKIGVGGHSYGAFTALLVGGV
                     RTFNAAGAPATYADPRVKAVVAMSPPGPGPSHGLTNQSWADLRLPVLFLTGTRDFGAT
                     ESEDLPWRKQAFELAPTGDKWFVSLNGVSAASFTGRVADVITYDTALPPSAYPGNRPV
                     PGGAPPQYTPPPDSRRGTGISFGQQNLINAVRIMSLAFWDTYLKNATAGREYLSKLKD
                     RGDAEVLSK"
     gene            1339..2028
                     /locus_tag="VF958_00785"
     CDS             1339..2028
                     /locus_tag="VF958_00785"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014796330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX9147670.1"
                     /translation="MKIEAARALVTGGSSGIGMATARELAARGAKVAICGRDEKKLRR
                     AAEELGVVAIHADVAAESDVVRMVREVLEKLGGYNVLINNAGFGEFAPLVDTTVADME
                     RVFGTNVMGAMLVARESARQFISQNYGNIVNISSSAGRRGFAGGTAYVASKFALSGMT
                     ECWRAELRKNNIRVMQINPSEVQTEFFQGRPFSQRKLVAADIARAIVSMLELEDRGFV
                     TEMSVWATNPD"
BASE COUNT          473 a          742 c          797 g          459 t
ORIGIN      
        1 gaattgagaa ttgagaattg agaattgaga attgcatgtc acgtttctca attttccatt
       61 ctctaatccg ctgattgtga tctgttcttg caaggccgga gccgggccca tccggctgca
      121 gaagaagtga ggtgttatga aacggacagt tctcctggtc ctctcctcca tcctgatcgc
      181 aacgtgcgcg agccagaaca ccaataaggt cgctaatgct gcaccgacgg cggcctctta
      241 cgtcgctgac tcgaagacca ttccggttgg agtcatcccc agcgcgatcg tccacgatgc
      301 cgtccggatt aaggatctcg agctgagcat cgactacccg gcgaaagacg gaccgtatcc
      361 ggtcatcatc ttctcgcccg gatacggttc ctccaatttg acctacgtcg gcacgtcgtc
      421 gttctgggca agccacggct acgtggtgat gaagccgaag catgctgatg ccggtgcgct
      481 gcgcccggcc gagaatgaga tgccggggga gaggccggag cagcgacgcg aaggccgccg
      541 cgatcgccgc cagcagaacg ccgatcagcc gccacagccg cgggtttgtc accccaatcc
      601 ggcggaggta tgggacgggc agacgccggc cgactggcag aatcgtgtgg ccgacatccg
      661 cctgatcctc gacaacctcg gaaagctcgt cgaacagtat cccgagctca aaccgcgact
      721 cgacgccacc aaaatcggcg ttggcggtca ttcctacggc gcatttaccg ctctccttgt
      781 cggcggcgtg cgcacgttca atgccgctgg cgccccagct acttatgccg atccgcgcgt
      841 gaaggccgtc gtggcgatgt cgccgccggg acccggtccg agccatgggc tgacgaacca
      901 gtcgtgggcg gatctccggc tgccggttct cttcctcacc ggcacgcgcg atttcggcgc
      961 gactgagtcc gaagacttgc cgtggcgcaa acaggcgttc gagctcgccc cgaccggtga
     1021 caaatggttc gtttcgttga acggcgtcag tgcggcttca ttcaccggtc gtgtggccga
     1081 tgtcattacg tatgacacgg ctcttccgcc ttccgcctat cccggcaatc gtccggtccc
     1141 gggaggcgcg cccccgcaat acacgccgcc gccggactcc cgccgcggaa ccggcatctc
     1201 attcggccag cagaatctga tcaatgctgt ccgcatcatg tcgctggcct tctgggatac
     1261 ctatctgaag aacgcaacgg ccggtcgcga gtacctgtcg aagctgaaag atcggggcga
     1321 cgcggaggtt ctgtcgaagt gaagatcgag gcagcaagag cgctggtgac gggaggcagc
     1381 tccggcatcg gaatggcgac ggcacgggag cttgcggccc gaggggcgaa ggttgccatc
     1441 tgcgggcgcg atgagaaaaa gctccgccgc gcagcagagg agcttggagt ggtcgccatt
     1501 cacgcggacg tggccgcaga gtccgacgtg gtccggatgg tgagggaagt cctcgagaag
     1561 ctcggcggct acaacgtcct gatcaacaac gccggcttcg gcgagttcgc gccgctcgtc
     1621 gacacgacgg ttgccgacat ggagcgggtg ttcggaacga acgtcatggg agcgatgttg
     1681 gtggcgcgcg aatcggcgcg ccaattcatc agtcagaact acgggaacat cgtgaacatc
     1741 tcgtcgagcg ccggccggcg cggctttgcc ggcggcactg cctacgtggc gagcaaattc
     1801 gccttgtccg gaatgaccga atgctggcgc gcggagctgc gcaagaacaa catccgcgtg
     1861 atgcagatca accccagcga ggtgcagacg gagttcttcc aaggccggcc gttcagccaa
     1921 cgcaaactcg tcgccgccga catcgcccgg gcgatcgtgt cgatgctcga gctggaagat
     1981 cgcggattcg tcaccgagat gtcggtctgg gcgacgaatc cggattgacg gttgccggtt
     2041 gcgggttgcc ggttgacggt tgacggttgc cggttgaccg tggggtttgt tggggttgcg
     2101 ttggggtttg ggaagttggc ggttgggttg gagggacgtt atcggccatc ggcgagccgt
     2161 gagaaacgga gagtccgcct ggcgggcttc cccggttcac gacatcgatc gaaccattcg
     2221 tcacgaataa ccgtcaaccc ggcaaccgtc aaccgtcaac cgtcaaccgt caaccccgtg
     2281 cgctagaatc ctctttcctc atggacgaaa agttgctgcc agaaggcgcg agttccctca
     2341 gagcgatgtg gaacgaggag ctcgagtcgg ccgagacgct gcagcgcaag ctgatggagc
     2401 agcggctcct ctccgaacag ctcgacctga tcggcagtgc ggtgcgggtg gcagggcagg
     2461 gaattgccat c
//
LOCUS       DASWAV010000037         2374 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U2665
            k141_442996, whole genome shotgun sequence.
ACCESSION   DASWAV010000037 DASWAV010000000
VERSION     DASWAV010000037.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960316
            Sequence Read Archive: SRR10260007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2374)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2374)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:47:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,929
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2374
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_442996"
                     /isolate="SMAG_U2665"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.36444 N 132.31889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..717)
                     /locus_tag="VF958_00790"
     CDS             complement(<1..717)
                     /locus_tag="VF958_00790"
                     /EC_number="2.3.1.9"
                     /inference="COORDINATES: protein motif:HMM:NF012336.4"
                     /note="Catalyzes the synthesis of acetoacetyl coenzyme A
                     from two molecules of acetyl coenzyme A. It can also act
                     as a thiolase, catalyzing the reverse reaction and
                     generating two-carbon units from the four-carbon product
                     of fatty acid oxidation; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HEX9147671.1"
                     /translation="MREVVILSAARTPVGSFLGKLSTLPVPALGSAAIRAAIERAGVR
                     PDEIQQVLMGNVLSAGVGQAPARQASLAAGVPDSVPCVTVSKVCGSGMRAVMDASNAI
                     RADEYEVAVAGGMESMSNAPYLLERARTGLRMGNGVLVDSMIKDGLWDPYSDVHMGSC
                     AEMCVSKYSFTREEQDAFARESYRRAQQAAQSGLAAEEIVPVDVPQKTGGGSAAARVD
                     ADEEPFAAPLEKIGTLKPAFQ"
     gene            759..1172
                     /gene="ruvX"
                     /locus_tag="VF958_00795"
     CDS             759..1172
                     /gene="ruvX"
                     /locus_tag="VF958_00795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020885747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HEX9147672.1"
                     /translation="MTRVLAVDFGEKRIGLATGDTFAGMATPRRTIPRSSDEAAAREI
                     ARFCREEQVETLLVGVPRSPEDGRESGIAPRIRSFARKLALAAALPLEFHEETLTSDE
                     AARRLRGRRSRAGIDAEAAAVLLEDWLAHRGHESA"
     gene            1169..2275
                     /gene="mltG"
                     /locus_tag="VF958_00800"
     CDS             1169..2275
                     /gene="mltG"
                     /locus_tag="VF958_00800"
                     /inference="COORDINATES: protein motif:HMM:TIGR00247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008932 - lytic endotransglycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HEX9147673.1"
                     /translation="MSAAKRLLRLGLLGVLLGVLVVFYLQHRMKHPPAAHVPGGDAIV
                     EIPPGTSTSEIFRRLDSAGVVEDGRLAEVYYRLHRRKTPLQAGEYRFHRPMPIDDIIN
                     MMGRGDVVRHAIVVPEGLTAEETFELFWRQGIGGPDAFKATLVQTELVPGLTKGVTDL
                     EGFLFPDTYVVTRSTSAAQIVDRMTANFREHFTPELQRKASALGLTTRQAVALASIVQ
                     KETALASEAPLVAGVYWNRLRRGMRLQADPTVIYALKRDGRWRGTLLRSDYDYESPYN
                     TYMHEGLPPGPIANPGFDALRAAVTPARTSYLYFVADPTTGGHTFSTTFEDHLVAVAL
                     ARRARDEIPARMGPETPVTPTPATETPVQTGAGQ"
BASE COUNT          375 a          842 c          795 g          362 t
ORIGIN      
        1 ctggaacgcg ggcttcaacg tgccgatctt ctcgagcggc gcggcgaagg gctcttcgtc
       61 ggcgtccacg cgcgcagcgg cggacccccc ccccgtcttc tgcggcacgt ccaccgggac
      121 gatctcctcc gccgccagac ccgactgcgc tgcctgttgc gcccgccggt acgactcgcg
      181 agcgaaggcg tcctgctctt cgcgggtgaa ggagtatttc gagacgcaca tctccgcgca
      241 gctccccatg tgaacgtcgc tgtacggatc ccagaggccg tccttgatca tcgagtcgac
      301 gagcacgccg ttgcccatgc ggaggccggt ccgggcgcgc tccagcagat acggcgcgtt
      361 ggacatcgac tccatgccgc cggcgaccgc gacctcgtac tcgtccgcgc gaatcgcatt
      421 cgaggcgtcc atcacggcgc gcattcccga accgcagacc ttcgagacgg tgacgcacgg
      481 aacggagtcc ggcacgcccg ctgcgagcga ggcctgtcgg gccggcgcct ggcccacgcc
      541 cgccgagagc acgttcccca tcaggacctg ctggatttcg tcgggacgaa ctcccgcgcg
      601 ctcgatcgcg gctcggatcg ccgccgagcc gagcgcgggc acggggagcg tggacagctt
      661 gcccagaaag gagccgaccg gcgtgcgggc ggcggaaagg atgacgactt ctctcacgcg
      721 gggattgtaa cgtcggggac gtcggttaac cttccctcat gacacgggtc ctggcggtcg
      781 acttcggcga gaaacgcatc ggtctcgcca cgggcgatac gttcgccgga atggcgacac
      841 cgcggcgcac gatcccgcgc tcgagcgacg aggccgccgc cagggagatc gcccgctttt
      901 gccgcgagga gcaggtcgaa acgttgctcg tcggcgtccc ccggtcgccg gaagacggcc
      961 gggaaagcgg gatcgcgccg cggattcgat cgttcgcccg gaagctcgcg ctcgccgcgg
     1021 ccctgcctct cgagttccac gaggagacgc tcacgtccga cgaagcggcc cggcgcctcc
     1081 gcggccgccg gtctcgcgcg ggaatcgacg ccgaggccgc cgccgtcctg ctcgaggact
     1141 ggctggcgca ccggggccac gagtcggcgt gagcgcggcg aagcggctcc tgcgcctcgg
     1201 tctcctgggg gtccttctcg gggtgctcgt cgtcttctac ctccagcacc gcatgaagca
     1261 ccctcccgcg gcgcacgtgc ccgggggcga cgccatcgtc gagattcctc ccggaacctc
     1321 gacgtcggag atcttccggc gcctggattc ggccggcgtc gtggaagacg gcaggctcgc
     1381 ggaggtctac taccgtctcc accggcgaaa aacgccgctg caggcggggg aataccggtt
     1441 ccaccgtccg atgccgatcg acgacatcat caacatgatg gggcgcggcg acgtcgttcg
     1501 gcacgcgatc gtcgtccccg aaggattaac ggccgaagag acgttcgagc tcttctggag
     1561 gcagggcatc gggggacccg acgcgttcaa ggccacgctc gtccagaccg agctcgtgcc
     1621 cggtttgacg aagggagtca ccgacctcga aggctttctc ttccccgata cctacgtcgt
     1681 cacccgctcg acctcggcgg cgcagatcgt ggaccgaatg acagcgaact tccgcgagca
     1741 cttcacgccg gagctgcagc gcaaggccag cgcgctcggg ctgacgaccc ggcaggccgt
     1801 cgcgctcgcc tcgatcgttc agaaggagac ggcgctcgca tccgaggctc ccctggtcgc
     1861 gggcgtctac tggaaccggt tgcggcgcgg gatgcgactc caggccgacc cgaccgtgat
     1921 ctacgcgttg aaacgggacg ggcgttggcg aggaacgctc ttgcgatcgg actacgacta
     1981 cgagtcgccg tacaacacgt acatgcacga aggactgccg ccgggaccga tcgccaatcc
     2041 cggcttcgac gctcttcggg cggccgtcac cccggcccgg acgagctacc tctacttcgt
     2101 tgcggacccg acgaccggcg gtcacacgtt ctccacgacg ttcgaggacc acctggtcgc
     2161 cgtggccctg gcccgccgcg cacgcgacga gatcccggcg cggatgggac cggagacgcc
     2221 ggtgacgccg acgccagcga cggagacgcc ggtgcagacg ggggcggggc agtagagccg
     2281 gtatcgcgcg accggtaccc gtctacttct cgtaggtgat cgtcttgatc cgcgggaact
     2341 tcgtccgcag gtagtcctcc atcaccttgt cctc
//
LOCUS       DASWAV010000038         5780 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U2665
            k141_602500, whole genome shotgun sequence.
ACCESSION   DASWAV010000038 DASWAV010000000
VERSION     DASWAV010000038.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960316
            Sequence Read Archive: SRR10260007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 5780)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5780)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:47:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,929
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5780
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_602500"
                     /isolate="SMAG_U2665"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.36444 N 132.31889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..614)
                     /locus_tag="VF958_00805"
     CDS             complement(<1..614)
                     /locus_tag="VF958_00805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9147674.1"
                     /translation="MTAFRLLLRALVTGPARRRPIRAVLPVFGVAVGVASVAAIQHAN
                     RSVAESFRESAQAIAGRSDLVVVGARGVPIADLDRLRFLWSVGSFAPLVSGNAVLGDG
                     SREVVALLGTDYGGDVAIRDMRLVAPSTAQGRMALSSPGSVLAPVPFARRHHLSIGSR
                     LLLVAGGVRAEVTVAGLLELSGLARASGGDVLVTDVFTAGRLLG"
     gene            complement(611..1237)
                     /locus_tag="VF958_00810"
     CDS             complement(611..1237)
                     /locus_tag="VF958_00810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015745589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX9147675.1"
                     /translation="MYEGAQPVVALDGVALKVSPGEFLGIVGPSGGGKSTLLHLVAGI
                     DRPTSGSIVVGGRDLASLSESQLTLYRRRDVGVVFQFFNLLAHLTVRENVELPRLLDG
                     RRDAAERAGELLDRVDLARRSDAHPYELSGGEMQRAAIARALVTGARLLLADEPTGNL
                     DSRHGEEVLALLDQVRRERGVTVMLATHSEAAARRADRVVELKDGRLA"
     gene            1337..2134
                     /locus_tag="VF958_00815"
     CDS             1337..2134
                     /locus_tag="VF958_00815"
                     /inference="COORDINATES: protein motif:HMM:NF013245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX9147676.1"
                     /translation="MLSPLRRAWRQRALVAALARRELVARYRGGVLGFFWSMLNPLLL
                     LLVYATVFRLVFAPRTDMRGPYALFLFAGVLVWGFVSSALLDAAETFRANGPLLRKTT
                     VAPEVFPAVVVAARLTHLALALPVLAAAVGVAAAMGAVVPTAAVLQFPVVLVLLAGTV
                     LGMAFVISVLSVHFGDVRDLLANLLTLAFFLTPVLYPIEAAPRRLRGFLWANPFTPFF
                     VAIHDSAFFFRPVPWQAWTGMLIAATLALALGGAVFERLRDSIAEEA"
     gene            2134..3390
                     /locus_tag="VF958_00820"
     CDS             2134..3390
                     /locus_tag="VF958_00820"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX9147677.1"
                     /translation="MDAPAIETRNVTKVYRRWGRKRSVGTFKSALLSGRPGHALAPDQ
                     GVPALTDVSFRVDPGETVGVVGANGSGKSTLLKLLAGVLKPTTGTVDVRGRLAALLEL
                     GAGFHPEISGRENVLINGLLLGLTKREVAARFDEIVRFAELEEFIDAPVKTYSSGMAV
                     RLGFAIAAHSDPDVLLVDEVLAVGDEAFARRSLEKFGEFERAGKTLLFVSHDLGLVAD
                     RCRRALWLDRGRLAADGPSAETIALYRENVSEREADRRGVAERPEPERPDRIGSGAAE
                     VGSVRLLGADGRPTARVRSGEPARLAMDVRAAAPLSDFVFGFAISTVSGTSVFGSNTA
                     LDGFAPEELAGDSTVVLEIESLDLAPGVYSVDAAVHRRDGAPYDYRRDLLRFEVAADR
                     AVGGVWNPARRWTFSGGVRWKSESRG"
     gene            3366..4106
                     /locus_tag="VF958_00825"
     CDS             3366..4106
                     /locus_tag="VF958_00825"
                     /inference="COORDINATES: protein motif:HMM:NF013718.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HEX9147678.1"
                     /translation="MEEREPRVAIVRRLFTRVEALGLYLMSGFLVCAVIVFLFGFLVR
                     EVFRTTDTREFDREVTLAVRELQSPSNDRIGRIVTFFGSHLFILPATVLITLLLRLKG
                     HWISALLFAGSVAGGFGLNALLKIAFARQRPDLWPALVAEMTYSFPSGHATMSTVFFG
                     GLAAVVLHLSRRPVVRGAALALATTAILMIAASRVYLGAHWTTDVTGGILVGLFWVSV
                     CSTGTRYVTERLAARVDLPVSRAAAPDV"
     gene            4103..5209
                     /locus_tag="VF958_00830"
     CDS             4103..5209
                     /locus_tag="VF958_00830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9147679.1"
                     /translation="MNGAAASRVRIALAAGFLFAAALRVGFLVSLRPNWDTNAYAAVV
                     RTLDSGGTLYRDTSLYNYSPAWAFTLRVLARIAGEGAIPFHRVVGAFLLLVDSISAWL
                     LFRVARDVLARPRETAMAAALLFFANPVSVFVTGFHAQFDGVAVAFLLLAIWLEGRSR
                     ARPPAAAAALAGSLLFKHVAAFHPIVFLRQRREGRRLSLPLVLLPYAVFCASFLPYWK
                     VRGAVLEKVFGYGSLAEDYGTAMLRRLPGVPQWAPSALFFAAAIFAALWLRRLEPARA
                     SLLLFLVLLVFAPGIAEYYFVWPIALGSLFGGAGYAVFTLVVSAFFLGSPDGLALPLT
                     HLPGWHGIWFGTVFWLLWEIRRVQGSKAGERKSL"
     gene            5206..>5780
                     /locus_tag="VF958_00835"
     CDS             5206..>5780
                     /locus_tag="VF958_00835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9147680.1"
                     /translation="MNERKVRPEILLVAAAVFGSLLVYAPFLGRMSDVYRFWDAPNYL
                     TIARTLYDVRPDNPLLAYVHTTRYFFVHLPMYPLTVRGLSFIGYEPALLLVSLLATCI
                     ATLLFYRLCRDVWKVASPAFLTLVFLFLPPRWLLYRSTGSTEAVYIALTLAAIFLFER
                     SHVARASVAGALATLTRISGLMLAPAFGMVLL"
BASE COUNT          724 a         2024 c         1966 g         1066 t
ORIGIN      
        1 ttccccagga gacgtcccgc ggtgaacacg tcggtcacga ggacgtcgcc tccggaagcc
       61 cgcgcgagac cggacagttc gagcagtccg gcgacggtga cttccgcgcg gacgccgccg
      121 gcgacgagca gcaggcgcga gccgatgctc aggtgatgcc ggcgcgcgaa gggaacgggc
      181 gcgagcacgg aaccgggaga ggacagggcc atgcggccct gcgccgtcga cggcgcgacc
      241 agtcgcatgt cccgaatcgc tacatccccg ccgtaatcgg ttcccagcag agcgaccacc
      301 tcgcgcgacc cgtcgccgag aacggcgttt cccgagacga gcggcgcgaa agagccgacg
      361 ctccagagga agcgcagccg atcgaggtct gcgatcggca ccccgcgcgc cccgacgacg
      421 acgaggtcgc tccttccggc gatcgcctgc gcggactcgc gaaaggactc ggcgacgctc
      481 cggttcgcgt gctggatcgc ggcgacggaa gcgacgccaa ccgcgactcc aaacaccggc
      541 aggaccgcgc ggatcgggcg gcggcgggcg ggacccgtga cgagagcccg gaggagaagg
      601 cgaaaagccg tcatgcgagc cgcccgtctt tgagttcgac gacgcggtcc gcacgtcgcg
      661 ccgcggcttc ggagtgggtt gcgagcatca ccgtcacgcc tcgctcgcgg cggacctggt
      721 cgagcagagc caggacttcc tcgccgtgcc gcgagtcgag attgccggtg ggctcgtcgg
      781 cgagaaggag ccgtgcgccg gtcacgagcg cgcgcgcgat cgcggcgcgt tgcatctccc
      841 cgccggacag ctcgtagggg tgagcgtcgc tccggcgggc gaggtccacg cgatcgagga
      901 gctcgccggc ccgctcggcg gcgtcgcgcc tcccgtcgag cagccgcggc agctcgacgt
      961 tctcgcggac ggtcagatgc gcgagcaggt tgaagaactg gaacacgacg ccgacgtcgc
     1021 ggcggcggta gagggtgagc tgcgattccg acaggctcgc gaggtcgcgt ccgcccacga
     1081 cgatggagcc ggacgtcggc cggtcgatgc ccgccacgag gtgcagaagt gtcgacttgc
     1141 cgccgccgga cggtccaacg atcccgagaa actcgccggg ggagaccttg agcgcgacgc
     1201 cgtcgagcgc cacgacgggc tgcgcgccct catacacttt cgcgacccgc tcacagacga
     1261 ccgcgggcgg cgatgaaggc gcctcgcgcg tctccggcga cgacacgcgc gggaggatat
     1321 cggatacttg tgacgcgtgt tgtccccact tcggcgcgcc tggcgacagc gggcgctcgt
     1381 cgctgctctc gcgcggcgcg agctcgtggc gcgctaccgg ggcggcgttc tcggcttctt
     1441 ctggtcgatg ctgaacccgc tcctgctcct cctcgtgtac gcgacggtgt tccgtctcgt
     1501 cttcgctccc cggacagaca tgcggggacc atatgcgctg tttctcttcg cgggggtatt
     1561 ggtctggggt ttcgtgtcct ccgcgctcct cgacgccgcg gagaccttcc gagcgaacgg
     1621 tcctcttctt cgcaagacga cggtcgcgcc ggaagtgttt ccggcggtcg tcgtcgccgc
     1681 ccgtctcact cacctcgcgc tcgccctccc ggtcctcgcg gcagccgttg gcgtcgccgc
     1741 ggccatgggc gccgtcgttc cgaccgccgc cgtgctgcaa tttccggtcg tgctggtcct
     1801 gctggcggga accgtcctcg gaatggcttt cgtgatctcg gtcctgtccg tccacttcgg
     1861 cgacgtccgc gacctgctcg cgaaccttct gacgcttgcg ttctttctga cgccggtgct
     1921 ctatcccatc gaggccgcgc cccggcgcct gcgcggtttc ctgtgggcga acccgttcac
     1981 gccgttcttc gtcgcgatcc acgacagcgc cttcttcttc cgtccggtgc cgtggcaggc
     2041 atggacggga atgctcatcg cggcgacgct cgcgctcgcg ctcggcggcg ccgttttcga
     2101 gcgacttcgg gactcgatcg cggaggaggc gtagtggacg cgccggcgat cgaaacgcgg
     2161 aacgtcacga aggtctaccg tcgctggggg cggaagcgct ccgtcgggac gttcaagtcc
     2221 gccctgctct ccgggcgccc gggccacgcg ctcgcgccgg accagggggt gcccgcgctc
     2281 accgacgttt cgttccgagt tgatccgggc gagaccgttg gagtcgtcgg cgccaacgga
     2341 tcgggaaagt cgacgctcct gaagctcctg gccggggtgc tcaagccgac gacgggaacc
     2401 gtcgacgtcc gcggccggct ggccgcgctg ctcgagctcg gggccggctt ccacccggaa
     2461 atatcgggga gggaaaacgt cctgatcaac gggctgctgc tcggcctgac gaagcgggag
     2521 gtcgccgcgc ggttcgacga gatcgtgcgt ttcgcggagc tcgaagagtt catcgacgcc
     2581 cccgtgaaga cgtattcgtc cgggatggcg gtccggctcg gattcgcgat cgccgcgcat
     2641 tccgatcccg acgtactgct cgtcgacgag gtcctcgccg tcggcgacga ggcgttcgcc
     2701 cgccgttccc tcgagaagtt cggggagttc gagcgtgcgg gcaagacgct cctcttcgtc
     2761 tcgcacgacc tcggtctcgt cgccgatcgt tgccggaggg cgctctggct cgaccggggc
     2821 cgtctcgcgg ccgacgggcc gtcggcggag accatcgctc tctaccgcga gaacgtgtcg
     2881 gagcgggagg cggaccggcg gggcgtggcg gagcgccccg agccggagcg cccggaccgg
     2941 atcggatcgg gcgccgccga ggtcggaagc gtacggctcc tcggcgccga tggtcgcccg
     3001 acggcgcggg tgcgttccgg cgaaccggcc cggctcgcga tggacgttcg cgccgcggcc
     3061 ccgctctccg atttcgtgtt cggcttcgcg atttccacgg tgtcgggcac gagcgtcttc
     3121 ggctcgaaca ccgccctgga cggtttcgcg cccgaagagc tcgccggcga ttcgaccgtc
     3181 gtgctcgaga tcgagtcgct cgacctcgcg cccggcgtct actcggtcga cgccgcagtg
     3241 cacagacgcg acggggcgcc gtacgattac cggcgcgacc ttcttcggtt cgaggtggcg
     3301 gccgatcgcg cggttggagg cgtgtggaac ccggcccgac gctggacctt ctcgggagga
     3361 gttcgatgga agagcgagag ccgcgggtag cgatcgtgcg gcgcctcttc acgcgcgtgg
     3421 aggcgctcgg gctctacctg atgtcggggt tcctcgtctg cgccgtgatc gtctttctct
     3481 tcgggttcct ggtgcgcgag gtgtttcgca cgacggacac gcgcgagttc gaccgggagg
     3541 tcacgctcgc ggtccgggag ctgcaatcgc cgtcgaacga tcgcatcggg cgaatcgtga
     3601 cgttcttcgg cagccacctc ttcattctcc cggcgaccgt tctcatcacg ctcctcctcc
     3661 ggctcaaggg ccactggatc tcggcgcttc tcttcgccgg ttcggtagct ggcggtttcg
     3721 gactgaatgc gctcttgaag atcgcgttcg ctcgccagcg gccggacctc tggccggcgc
     3781 tcgtcgcgga aatgacctac agctttccga gcgggcacgc gacgatgagc acggtgtttt
     3841 tcggagggct cgctgccgtc gtcctccacc tgtcgcgccg gccggtcgtc cgaggagcgg
     3901 cactcgccct cgcgacgacg gcgatcctga tgatcgccgc gagccgcgtc tatctcggag
     3961 cgcactggac gaccgacgtg accggaggga tcctcgtcgg gctcttctgg gtttccgtct
     4021 gctcgacggg gacgcggtac gtgacggagc gcctggcggc aagagtcgac ctgccggtct
     4081 cgcgagcggc cgctcccgac gtgtgaacgg agcggccgcc tcgcgggtcc ggatcgcgct
     4141 cgccgccggg tttctgttcg cggccgcgct gcgggtcggt tttctcgttt cgctccgtcc
     4201 gaactgggac accaacgcct atgctgccgt cgtccgcacg ctcgactccg gcgggacgct
     4261 ctaccgcgac acctccctct acaactactc gcccgcctgg gcgttcacgc tccgagtcct
     4321 ggcgcggatc gccggggaag gcgcgattcc gtttcaccgg gtcgtcgggg cgtttctcct
     4381 cctcgtggat tcgatcagcg catggctcct gtttcgcgtg gcccgcgacg tgctcgcccg
     4441 gccgcgggag actgccatgg ccgcggcgct cctcttcttc gcgaaccccg tttccgtttt
     4501 cgtgaccggg tttcatgcgc agttcgacgg cgtcgcggtc gcctttcttc tcctcgcgat
     4561 ctggctggag gggcgttcgc gcgctcgacc gccggctgcg gccgcggctc tcgcgggatc
     4621 gctcctcttc aagcacgtgg ccgcgtttca tccgatcgtt ttcctccgcc agcgtcgaga
     4681 ggggcgccgg ctcagccttc ctctcgtcct gttgccttac gccgtcttct gcgcttcctt
     4741 cctgccgtac tggaaggtgc gcggcgccgt cctcgagaag gtcttcggat acggcagcct
     4801 cgcggaggac tacgggaccg cgatgctccg gcgtctgccc ggcgtgccgc agtgggcacc
     4861 gtcggcgctc ttcttcgccg cggcgatctt cgctgcgctg tggcttcggc ggctcgagcc
     4921 cgcgcgggcg tcgctgctcc tgtttctcgt cctcctcgtc ttcgctcccg gaatcgcgga
     4981 gtactacttc gtgtggccga tcgcgctcgg ctcgctcttc ggcggcgcgg gttatgctgt
     5041 cttcacgctc gtcgtctccg catttttcct cggatcgccc gacggtctcg cgttgccgct
     5101 gacgcacctg ccgggctggc acggcatctg gttcggaacc gtgttctggc tgctgtggga
     5161 gatccgacga gtccagggct cgaaggccgg agaacggaag agcctgtgaa cgagcggaaa
     5221 gtccgcccgg agatcctgct ggtagcggcg gcggtcttcg ggtctctgct cgtctacgcg
     5281 ccgtttctcg gccgaatgag cgacgtgtac cggttctggg acgccccgaa ctacctgacg
     5341 atcgcgcgga ctctctacga cgtccgcccg gacaatccgc tgctcgcata cgtccacacg
     5401 acccggtatt tcttcgtcca cctgccgatg tatccgctca cggtccgcgg gctctccttc
     5461 atcggctacg agccggcgct gcttctcgtt tcccttctgg cgacgtgtat cgcgacgctc
     5521 ctcttctacc gcctgtgccg cgacgtctgg aaagtggcgt cgcccgcgtt cctgacgctc
     5581 gtttttctct tcctgccccc gcgctggctc ctttaccggt caaccggctc gaccgaggcc
     5641 gtctatatcg cgctcacgct cgccgcgatc ttccttttcg agcgctccca cgtcgcgcgc
     5701 gcttcggttg cgggcgctct cgcgacgctc acacgcatct cgggcctcat gctcgcgccc
     5761 gcgttcggaa tggtgctgct
//
LOCUS       DASWAV010000039         3116 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U2665
            k141_642204, whole genome shotgun sequence.
ACCESSION   DASWAV010000039 DASWAV010000000
VERSION     DASWAV010000039.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960316
            Sequence Read Archive: SRR10260007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 3116)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3116)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:47:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,929
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3116
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_642204"
                     /isolate="SMAG_U2665"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.36444 N 132.31889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..296)
                     /gene="glyA"
                     /locus_tag="VF958_00840"
     CDS             complement(<1..296)
                     /gene="glyA"
                     /locus_tag="VF958_00840"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013349948.1"
                     /note="catalyzes the reaction of glycine with
                     5,10-methylenetetrahydrofolate to form L-serine and
                     tetrahydrofolate; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HEX9147681.1"
                     /translation="MNRSTLSTSAAAFLDRDLEVVDPEVARWIHDEELRQNGGLELIA
                     SENFVSRAVREAQGSVLTNKYAEGYPGRRYYGGCEFVDKIEALAIERVKKLFGA"
     gene            complement(319..1593)
                     /gene="dgt"
                     /locus_tag="VF958_00845"
     CDS             complement(319..1593)
                     /gene="dgt"
                     /locus_tag="VF958_00845"
                     /inference="COORDINATES: protein motif:HMM:TIGR01353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008832 - dGTPase activity [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dNTP triphosphohydrolase"
                     /protein_id="HEX9147682.1"
                     /translation="MTVATSPSTREHFERLEEERLAIYASRSARATRPHGPLSADEQD
                     FRTQYVRDRDRIIHSRAFRRLKHKTQVFIPFEGDHFRTRLTHTIEVSQIARSVARALG
                     LNEDLTEAVALGHDLGHTPFGHSGENVLDALLSESHPQAGRFKHNYQSVRVVDRLERR
                     YADPGLNLTHDVREGIFKHTTWKRDFPFPLDFRDGLRMESGGTLEAQVVNWSDEIAQQ
                     THDLEDGISLAGEEGAEALAVSRLARAVRGGGDEPAVRRASLIRGMITTLISDLVAAS
                     RARIERWLDASRISTPDEFFARHEHLPGDLVGFSDEGRRLYTELKEFVYRHIIHSFPV
                     SRHDGRARRVVAGLYRAYAENPRLLPDSVLEVLRQELQIRFLREVPLSEIKAETAANY
                     LPRPEFYRAIADHIAGMTDSYCIADYRELVTE"
     gene            complement(1590..2834)
                     /locus_tag="VF958_00850"
     CDS             complement(1590..2834)
                     /locus_tag="VF958_00850"
                     /inference="COORDINATES: protein motif:HMM:TIGR01499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HEX9147683.1"
                     /translation="MKLGLAAIDSVCERLGHPEAAAPSVLVAGTTGKGSVAAMLSAIA
                     ESGGVRAGLYTSPHLARAGERIRIEEADVSDGELDSFLAPVFHAADARPEVSLTYFEA
                     LTAAAFLAFAEQNLDLAVLEIGLGGRYDATNIAPARLSIVTTIGLDHVEELGPTVEAI
                     AREKAGVFRAGRPALIGACPDEARLALTRVARETGALLHDAADEISLGDVTTSLEGTR
                     FRLTTPLNLYQLATPLPGAHQAANAALATRAAELLSGELHGFEERAIARGLASTRWPG
                     RLEKLRVRGRTVILDGCHNPQGAEALARFLRDANLAGRARLVFGAMADKDVEQMSAIL
                     FPSVATVHLVTAASQRAATAEDLLRRTAHARSGSIAAPDFGSALAELLRDPDPAPIIV
                     AGSLYLVGEARERLLSGRFEDE"
     gene            complement(2942..>3116)
                     /locus_tag="VF958_00855"
     CDS             complement(2942..>3116)
                     /locus_tag="VF958_00855"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013615376.1"
                     /note="catalyzes the carboxylation of acetyl-CoA to
                     malonyl-CoA; forms a tetramer of AccA2D2 subunits; Derived
                     by automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyl transferase
                     subunit beta"
                     /protein_id="HEX9147684.1"
                     /translation="QTIRQTLPEGFQRSEFLLDHGMLDFVVDRSEMKETLVRCLELLR
                     VGAGGRKSEIGNR"
BASE COUNT          496 a         1014 c         1114 g          492 t
ORIGIN      
        1 tcggcgccga agagcttctt cacgcgctcg atcgcgaggg cctcgatctt gtcgacgaac
       61 tcgcagcccc cgtagtaccg gcgacccgga tatccctccg cgtacttgtt cgtgaggacc
      121 gacccctgcg cctcgcgcac cgcgcgagac acgaagttct ccgacgcgat gagctcgagg
      181 ccgccgttct ggcggagctc ctcatcgtgg atccagcggg cgacctccgg gtccacgacc
      241 tcgaggtcgc ggtcgaggaa cgccgccgcg gaagttgaga gtgtcgaacg gttcatctcc
      301 gccgcctttc cggagtcgtc actccgttac gagctcgcgg tagtccgcga tgcagtagct
      361 gtcggtcatt cccgcgatgt ggtctgcgat tgcgcggtag aactcggggc gcgggaggta
      421 gttcgccgcc gtctcggcct tgatctccga gagtggaact tcgcggagga agcggatctg
      481 cagttcctgc cgcaaaacct ccaggacgga gtcgggaagg aggcgggggt tctcggcgta
      541 cgcgcgataa aggcccgcga cgacgcgccg ggcgcgcccg tcgtgacgcg agaccggaaa
      601 cgagtgaatg atgtggcggt agacgaactc cttcagctcc gtgtaaagcc ggcgcccctc
      661 gtcggaaaag ccgacgagat ctccgggcag gtgttcgtgc ctcgcgaaga actcgtcggg
      721 tgtcgagatg cgactggcgt cgagccaccg ctcgatgcgc gcgcgcgacg cggcgacgag
      781 gtccgagatg agggtcgtga tcattccgcg gatgagcgag gcgcggcgca cagccggctc
      841 atctccgccg ccccgcaccg cgcgcgcgag gcgcgagacc gccagcgcct cggcgccttc
      901 ctcgcccgcg agcgagattc cgtcttcgag gtcgtgcgtc tgctgggcga tctcgtcgga
      961 ccagttgacg acctgcgctt cgagagtccc cccggattcc atccgcaggc cgtcccggaa
     1021 gtcgagcggg aacgggaagt cgcgcttcca cgtcgtgtgc ttgaagattc cctcccgaac
     1081 gtcgtgcgtc aggttcagcc ccggatccgc gtagcgacgc tcgaggcggt ccacgacccg
     1141 caccgactgg tagttgtgct tgaaccggcc cgcctgaggg tggctctccg agagaagcgc
     1201 atcgagcacg ttctccccgg aatgtccgaa cggcgtgtgg ccgaggtcgt ggccgagggc
     1261 gacggcttcg gtcaggtcct cgttcaggcc gagggcgcgc gcgacgctcc gcgcgatctg
     1321 cgagacctcg atcgtgtgcg tgaggcgggt gcggaaatgg tccccctcga acgggatgaa
     1381 gacctgggtc ttgtgcttca gccggcggaa cgcccgcgaa tgaatgatcc ggtcgcggtc
     1441 ccggacgtat tgcgtgcgga agtcctgctc gtccgcggac aggggaccgt ggggccgggt
     1501 cgcgcgcgcg cttcgtgaag cgtagatcgc gagccgctcc tcctcgaggc gctcgaagtg
     1561 ctcccgggtg ctgggcgatg tcgcgaccgt cattcgtcct cgaatcgtcc ggagagaagg
     1621 cgctcgcgcg cctcgccgac gagatagagc gatccggcca cgattatagg ggcgggatcg
     1681 gggtcccgca ggagctccgc gagcgcggac ccgaagtccg gggcggcgat ggagccggag
     1741 cgagcatgag cggtgcgccg gagaaggtct tccgccgtgg cggcgcgctg cgaagcggca
     1801 gtgacgagat gcacggtcgc caccgacgga aacaggatcg ccgacatctg ctcgacgtcc
     1861 ttgtccgcca tcgccccgaa gacgagcctc gcccggccgg cgagattcgc gtcccggagg
     1921 aaccgggcga gcgcctcggc gccctgaggg ttgtgacagc cgtcgagaat caccgtgcgg
     1981 ccgcggacgc ggagcttttc cagccgcccg ggccagcggg tcgaggcgag tccgcgcgcg
     2041 atcgccctct cctcgaaccc gtgaagctct ccggacaaga gctccgcggc ccgggtcgcg
     2101 agggcggcgt tggcggcctg atgggcgcca gggaggggcg tcgcgagctg gtataggttg
     2161 agcggcgtcg tgagacgaaa gcgcgtaccc tcgagcgacg tggtgacgtc gccgagcgag
     2221 atttcgtcgg cggcgtcgtg gaggagcgcc cccgtctcgc gagcaactcg ggtaagcgcg
     2281 aggcgcgctt cgtccggaca cgccccgatc agcgccggcc gcccggcgcg aaagacgccg
     2341 gccttctcgc gcgcgatcgc ctcgacggtc ggtcccagct cctcgacgtg gtcgagcccg
     2401 atggtcgtga cgatcgaaag acgggccggc gcgatgttcg tggcgtcgta gcgtcccccg
     2461 aggccgatct cgagaaccgc gaggtcgagg ttctgctcgg cgaacgccaa gaacgcggcg
     2521 gccgtgagcg cctcgaagta cgtgagtgag acctccggcc gcgcgtccgc agcatggaag
     2581 acgggggcga gaaaggaatc gagctccccg tcggagacgt ccgcctcctc gatccggatc
     2641 ctttctccgg cgcgggccag gtggggggag gtgtagagcc ccgcccgcac accgccggac
     2701 tccgcgatcg ccgacagcat cgcggcgacc gacccttttc cggtggttcc ggcgacgagc
     2761 acggacggag cggccgcctc cgggtggccc aggcgctcgc agaccgagtc gattgccgcg
     2821 aggccgagct tcattccccg aggtgcgaga gattcgagac gtgccagagc cggaaatcgg
     2881 gaatcgggat tgggagatcg agggaaggga gaagtggcga tcgtcgtgcc cggctcttcg
     2941 attaccgatt accgatttcc gatttccgac ccccggctcc cacgcggaga agctccaggc
     3001 atctcacgag cgtctccttc atctcggatc ggtcgacgac gaaatcgagc attccgtgat
     3061 cgaggaggaa ttccgagcgc tggaagcctt cggggagcgt ctgccggatc gtctgt
//
LOCUS       DASWAV010000040         6161 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U2665
            k141_41915, whole genome shotgun sequence.
ACCESSION   DASWAV010000040 DASWAV010000000
VERSION     DASWAV010000040.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960316
            Sequence Read Archive: SRR10260007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 6161)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6161)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:47:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,929
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6161
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_41915"
                     /isolate="SMAG_U2665"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.36444 N 132.31889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..245)
                     /locus_tag="VF958_00860"
     CDS             complement(<1..245)
                     /locus_tag="VF958_00860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011687573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin domain-containing protein"
                     /protein_id="HEX9147685.1"
                     /translation="MALRINSEAPNFTADTTQGPIKFHEWIGEGWAILFSHPKDFTPV
                     CTTELGYMAGLAPEFARRNTKIIGLSVDPVDSHAKWA"
     gene            complement(279..755)
                     /locus_tag="VF958_00865"
     CDS             complement(279..755)
                     /locus_tag="VF958_00865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014214511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HEX9147686.1"
                     /translation="MSADTTSGEGHVVVRGPASGFAQEIVAGRHLFAGDEPLTAGGTD
                     TGPTPYDHLLAALGSCTSMTVGLYARRKSWPLESVTVRLTHSRIHAVDCADCETKEGK
                     LDRIDVVIELTGGLTGEQRARLLEISERCPVHRTLRSEIDIRSGLAKPEIPSSPGL"
     gene            complement(752..2422)
                     /locus_tag="VF958_00870"
     CDS             complement(752..2422)
                     /locus_tag="VF958_00870"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011681849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047420 - N-acyl-D-amino-acid deacylase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacylase"
                     /protein_id="HEX9147687.1"
                     /translation="MFPSLLVIVLGLPLAAAPASTTFDMIFAGGHVVDGTGAPWFRAD
                     VGIAGDRIAAVGDLSRAAAKRRVDVSRLVVAPGFIDMLGQSEYNVLVDNRAASKITQG
                     ITTEITGEGSAIAPLNARMIADRKDAWSRYGVTPDWTTLAGYWKAFERARPALNLGTF
                     VGAGGVRDLVVGKDDRAATPSELAAMETAVARAMEEGAFGLSTSLAYVPDRFASTEEI
                     IALAKVAARYGGTYITHQRDEGDGIDASLDEVFSIAREARIPAQIYHLKTAGKRNWGR
                     MPAVLRRLEQARAEGLDVSANQYPWVASANALSASLPLWAREGGREKLLARLRDPSAR
                     ARVRADFPRNNPDWPDQPGSRILITTVLKPELKKYEGKTIAEIARAEEKDPLDVLMDL
                     VIADNANVGRVTFSMSEEDVKAALAHPLVSMGTDSGARAEDGIYSLEKSHPRAWGSTA
                     RILGKYVREEKVLTLEEAVRKMTSLPASRMGLADRGIVRPGMAADVVVFDPATVRERS
                     TYADPLHYSEGIPFVCVNGQLVVDGGRITGARPGRALKGPGYRGGGAR"
     gene            2457..4193
                     /locus_tag="VF958_00875"
     CDS             2457..4193
                     /locus_tag="VF958_00875"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HEX9147688.1"
                     /translation="MTRLSPTSLSTEVPARQDSRPKRAALLVIVLVCLTVYNSNFRLI
                     GSGDSFPARLLPFEILTNGTLVFDSLGSPPSGAYWFMYARGGHLVSRYPIVLPVLVTP
                     LFVPAAIYVAALKPNFWRFETILELMEKGVASLIAALSVAVIWLTLRRLTGRKMALLL
                     AVAYAFGTQTWSTSSQALWQHGMGELLFASTLYLLVRCGSSPSAALAVGALCGLMAFN
                     RPPNALFAAAVAVFYVLRGRKGLPRFALGALALSLPFLVYNLYYFGRPLGGYQSLVGS
                     TVFQHSIPDGVAAMLVSPGKGMLVFAPFFLFLALGRGLELGAAGRLPVLLFWLAFLGQ
                     LVLYGMTDWRAGWCYGPRFLTDVLPFLTVALVPVVERLRPAALALFCAFVAFAIFVQA
                     IGAFYFPSGGSYLLSKTDFWKPSNAQFLVELRGGPSRLEFAPRIARWIRRMVHGRPAE
                     VVSRSTFFYTVMPCRAVDTREIPGPFGGPPLRANSNRFIRLAGRCGVPSTARAVAANL
                     TVLGASAPGELKLLSASDFEAGASAISYRAGQTRANNGIFPLDAGGSLGLSCEQNSGA
                     VHVLVDVNGYFE"
     gene            4269..>6161
                     /locus_tag="VF958_00880"
     CDS             4269..>6161
                     /locus_tag="VF958_00880"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HEX9147689.1"
                     /translation="MMNTRLGNRYEVAAELGRGGMGVVYRSRDPLLNREVAVKVLATS
                     ELTPEIEERFQREAQIVAQMDHPGIVPIYDVGRHDGSLFFVMPLVEGTNLRRLLWDGS
                     LRLGEVLDIAIQVAEALEYSHSRGVVHRDIKPENIMVAREEGGSARVRVMDFGLAHAT
                     AENRLTKTGTLVGTVNYLSPEQVTSHVFDGRSDIYSLGVVIYECLTGEPPFTGEVQSV
                     LYRIVHEIPQPPRALGAEIREELQNIILQCLEKDPARRPQKAGQVSDELRRLRSTLHS
                     DEFAMSVVGTASRTLQRPSASAFVGREKEFAELQRRLNAAVSGDCQFAVVSGEPGIGK
                     TRLLEELKKLVSMRKIRVLYGRFVEQDQAFSYQGFCELVQDYFRSRDPGSSAAERPDF
                     SDLAPELLSLFPLLSEISELRAAASGDSRVASPAAERKAEDRIQIFELLARTLTRMAA
                     GKPLVLILENLHAAEVSIEALQYIVRRLGPTPTLFVGSYRQTEVDKRHPLSRMLDSFA
                     DDPRFVSITLGPLSASEHRALVASVVGAQNVSDELAHRVHEATEGNPFFTKELLRSLI
                     DAGGIARDDTGEWNFSQGSGISSDALPATIQQAVEKRIERLPEDLRDLLSVASVLGRS
                     FDFRDLE"
BASE COUNT          916 a         2149 c         1952 g         1144 t
ORIGIN      
        1 ttcgcccact tcgcgtgcga atcgaccggg tcgacggaca ggccgatgat cttcgtgttg
       61 cgcctggcga actccggcgc gagtccggcc atgtagccga gttcggtcgt acagacgggc
      121 gtgaagtcct tcgggtggga aaagaggatg gcccagcctt cgccgatcca ctcgtgaaat
      181 ttgatcggtc cctgggtcgt atccgcggtg aagtttggag cctcgctgtt gattcggaga
      241 gccatgaacg acctcccttc gtctctgtcc ggccgagatc atagcccggg cgaggacggg
      301 atctccggct ttgccagccc gcttcggatg tcgatctcgg atcggagtgt ccggtgcacg
      361 gggcaccgct ccgagatctc cagcaaacgc gctcgttgtt caccggtgag cccgcctgtg
      421 agctcgatca ccacgtcgat ccggtcgagc ttcccctcct tcgtctcgca gtccgcgcag
      481 tccacggcat ggatgcgcga atgcgtgagg cgtaccgtca cggattcgag gggccagctc
      541 ttccgacgcg cgtagagccc gacggtcatc gaggtgcagc ttccgagagc ggccagcagg
      601 tggtcgtacg gagtcgggcc ggtatcggtg ccgccggcag tcagcggctc gtcccccgcg
      661 aagaggtggc ggccggccac gatttcctga gcaaacccgg aggccgggcc ccggacgacg
      721 acgtggccct cgccggaggt cgtgtccgcg ctcatcgtgc acctcctccc cggtaacccg
      781 gtcctttgag cgcccggccc ggccgcgcgc cggtgatcct cccgccgtcc acgacgagct
      841 gcccgttcac gcacacgaac ggaatccctt cgctgtagtg cagcggatcg gcatacgtcg
      901 agcgctcgcg caccgtcgcc gggtcgaaca cgacgacgtc cgcggccatc ccgggtctca
      961 cgatgccccg atccgcgagc cccatccgag acgccggcaa cgacgtcatt tttcgcaccg
     1021 cctcttcgag ggtcagcacc ttctcctcgc gaacgtattt ccccaggatc cgcgccgtcg
     1081 agccccaggc gcgcgggtgc gatttctcga gggagtagat cccgtcctcg gcccgcgccc
     1141 cggagtccgt ccccatggaa accaacgggt gagcgagggc agctttgacg tcctcctcgc
     1201 tcatcgagaa cgtgacccga ccgacgttcg cgttgtccgc gatgacgaga tccatgagga
     1261 cgtcgagtgg gtccttctcc tccgcgcgcg cgatctccgc tatggtcttg ccttcgtact
     1321 tcttgagctc gggcttcagc accgtcgtga tcaggatgcg agagcccggc tggtcgggcc
     1381 agtccgggtt gttccgggga aagtcggcgc gcacgcgggc gcgcgcggac gggtcccgca
     1441 ggcgggcgag gagcttctcc cgccccccct cgcgcgccca gagcggcagg ctcgccgaga
     1501 gggcgttcgc gctcgccacc caggggtact ggttggcgga gacgtcgagg ccttcggccc
     1561 gcgcctgctc gagacgtcgg agcacggccg gcatccggcc ccagttgcgc ttgcccgcgg
     1621 tcttcaggtg gtaaatctgc gcggggatcc gcgcctcgcg cgcgatcgag aacacctcgt
     1681 ccaggctcgc atcgattccg tccccttcgt cacgctgatg ggtgatgtac gtgccgccgt
     1741 aacgcgccgc gaccttcgcg agcgcgatga tctcctccgt cgaggcaaag cgatcgggga
     1801 cgtacgcgag tgaggtcgag agcccgaacg cgccctcttc catcgcccgg gcgaccgcgg
     1861 tctccattgc cgcgagctcc gacggcgtcg ccgcccggtc gtcctttccg acgacgaggt
     1921 cccggacgcc ccccgctccg acgaacgtcc cgaggttcaa tgccggccga gcgcgttcga
     1981 atgccttcca gtagcccgcg agcgtcgtcc agtccggcgt gacgccgtag cggctccaag
     2041 cgtctttccg gtcggcgatc atccgggcgt tcagcggggc gatcgcgctg ccctcgccgg
     2101 tgatctcggt cgtgatcccc tgcgtgatct tgctcgccgc gcggttgtcg acgagcacgt
     2161 tgtactcgga ctggccgagc atgtcgatga agcccggcgc gacgacgaga cgcgagacgt
     2221 cgacccttcg cttcgcggcg gcgcgcgaaa ggtctccgac cgcggcgatg cgatcacccg
     2281 cgatccccac gtccgcgcgg aaccacggcg cgcccgttcc gtcgacgacg tgcccgccgg
     2341 cgaagatcat gtcgaaggtc gttgaggcgg gggcggccgc gagcggcagg ccaagcacga
     2401 tcacgaggag agacggaaac atcgaaacgt catgctaacc tcgcgggaga gcagaattga
     2461 cgcgactgtc tcccacctct ctttcaacag aagtacccgc gcgtcaggat tcacggccga
     2521 aacgtgcggc cctcctcgtc atcgttctcg tctgcctcac ggtctacaac tcgaacttcc
     2581 ggctgatcgg ctcgggagat tcgtttccgg cccgcctcct tcccttcgag atcctgacga
     2641 acggaactct ggttttcgac tcgctcggct cccctccatc cggcgcctat tggttcatgt
     2701 atgcgcgcgg cggccacctc gtgtcgcggt acccgatcgt gctgccggtg ctcgtcacac
     2761 cgctcttcgt gcccgcagcg atctacgtcg cggctctgaa gccgaacttc tggcgattcg
     2821 agacgatcct cgagctgatg gagaagggcg tcgcgtccct gatcgccgca ctgtcggtcg
     2881 ccgtcatttg gctgaccctt cgccgtctga ccggcaggaa aatggctctc ctcctcgcgg
     2941 tcgcctacgc cttcggaacc cagacgtggt cgacgagctc tcaggcgctc tggcagcacg
     3001 gcatgggaga gctcctgttc gcgtcgactc tttacctgct cgtgcggtgt ggttcttcgc
     3061 cgagcgccgc gttggccgtt ggggccctgt gcggcctcat ggcgttcaat cgtccgccga
     3121 acgccctctt cgccgcggcg gtcgcggttt tctacgtgct ccgcggccgg aagggattgc
     3181 cgcgcttcgc gctcggcgct ctcgcactct cccttccgtt tctcgtctac aacctgtact
     3241 acttcgggag accccttggc ggataccagt cgctcgtggg ctccaccgtc tttcagcact
     3301 cgattcccga cggcgtcgcc gcgatgctcg tgagcccggg caaaggtatg ctcgtcttcg
     3361 ccccgttctt tctcttcctg gcgcttggcc gagggcttga gctcggcgcc gccgggcggc
     3421 tgccggttct actgttctgg ctcgccttcc tcggacagct cgttctctac gggatgacgg
     3481 actggcgcgc cggctggtgc tacgggccga ggttcctgac cgacgtcctc ccctttctga
     3541 ccgtcgcact cgtccctgta gtcgagcggc tccgtcccgc cgcgctcgcc ttgttctgcg
     3601 cgttcgtcgc gttcgcgatt ttcgtccagg cgatcggcgc cttctacttc ccgtccggcg
     3661 gaagctacct cctctccaag acggacttct ggaagccctc aaatgcccag ttcctggtcg
     3721 aactccgagg ggggccgagt cgtttggagt tcgcgccgcg catcgcgaga tggatccggc
     3781 ggatggttca tggccgtccc gcggaggtcg tctctcgatc aacgttcttc tacacggtga
     3841 tgccctgccg cgcggtggat actcgggaga tccccggccc gttcggcggt ccacccctgc
     3901 gggcgaattc gaaccgtttc atccggctcg ccggccgttg cggggtgcct tcaaccgcaa
     3961 gagccgtcgc ggccaacctg acggtgctcg gcgcttcggc gccaggcgag ctgaagctcc
     4021 tgtcggcctc cgacttcgag gcgggcgcct ccgcgatcag ctaccgcgcc gggcagaccc
     4081 gggcgaacaa cggcatcttc ccgctggacg ccggcgggtc gctcgggctc tcgtgcgagc
     4141 agaactcagg cgccgttcat gttctcgtcg acgtcaacgg ctacttcgaa tgatcccggt
     4201 gtgaacaccc gccgcggacc tgataatctt ccgcctcccg cgtctccaca ggagtgactg
     4261 ccccggccat gatgaacacc cgactgggca accgatacga agttgcggcc gagctcggcc
     4321 gcggcgggat gggagttgtc tatcgatccc gcgatccgct cctgaaccgc gaggtcgccg
     4381 tcaaggtcct cgccacttcc gagctcacac ccgagatcga ggaacgcttc cagcgcgagg
     4441 cccagatcgt cgcgcagatg gatcaccccg gtatcgtccc gatctacgac gtcggtcggc
     4501 acgacggctc gctcttcttc gtgatgccgc tcgtcgaggg cacgaatctc cggcggctcc
     4561 tctgggacgg ctcacttcgt ctgggcgaag tcctcgacat cgcgatccag gtcgcggagg
     4621 cgctcgagta cagccactcg cgcggcgtcg tccaccgcga catcaaaccc gagaacatca
     4681 tggttgcgcg cgaggaagga ggatccgcgc gggtccgcgt catggatttt ggactcgccc
     4741 acgcgaccgc ggagaaccgg ttgacgaaga cgggcacgct cgtcggtacg gtcaactatc
     4801 tctcccccga gcaggtgacc tcgcacgtct tcgacggccg ttcggacatt tactcgcttg
     4861 gcgtcgttat ctacgagtgc ctgacgggcg aaccgccgtt caccggtgaa gtccagtcgg
     4921 tcctctaccg gatcgtgcac gagatcccac agccgccacg agccctcgga gccgagatcc
     4981 gcgaggagct gcagaacatc attctgcagt gcctcgagaa ggacccggcg cggcgtccgc
     5041 agaaggccgg gcaggtctcc gacgagctcc ggcggctccg ctcgactctc cacagcgacg
     5101 agttcgcgat gtcggtcgtg gggaccgcga gccgcacgct gcaacgcccg tccgcgtccg
     5161 cgttcgtcgg gcgcgagaag gagttcgccg agctccagcg gcgcctgaac gccgcggttt
     5221 ccggcgactg ccagttcgcc gtcgtctcgg gagaaccggg gatcgggaag acgcggctgc
     5281 tcgaagagct gaagaaactc gtctcgatgc gcaagatccg cgtcctctac gggcgattcg
     5341 tcgagcagga ccaggccttc tcctaccagg gattctgcga actcgttcag gactacttcc
     5401 gctcccggga cccgggaagc tccgcggccg agcggcccga cttctcggat ctcgcaccgg
     5461 agctcctctc gctctttcct ctgctgtccg agatcagcga gctgcgtgcg gccgcgagcg
     5521 gagattcgcg ggtcgcttct cccgccgcgg agcggaaagc ggaagaccgg attcagatct
     5581 tcgagctgct ggcgcgaacg ttgacccgga tggcggccgg gaagcctctc gtcctcatcc
     5641 tcgaaaacct tcatgccgcc gaagtctcga tcgaggcgct ccagtacatc gtgcgccgac
     5701 tcgggccgac gccgaccctg ttcgtcggca gctatcggca gaccgaagtc gacaagcgtc
     5761 accccctttc gcgcatgctc gactcgttcg cggacgaccc gcggttcgtc tccatcacgc
     5821 tcggcccgct ctcggcgagc gaacaccggg cactggtcgc ctccgtcgtc ggcgcgcaga
     5881 acgtgtccga cgagctcgcg caccgcgttc acgaagcgac ggaaggcaac ccgttcttca
     5941 cgaaggagct gttgcggtcg ctcatcgacg cgggcggtat cgcgagggac gacacgggcg
     6001 agtggaactt ttcgcaggga tcggggatct cgtccgacgc cctgccggcg acgatccagc
     6061 aggccgtcga aaagaggatc gagcggcttc cggaagatct gcgtgatctc ctttcagtcg
     6121 cctcggtcct gggtcgaagc ttcgacttcc gagacctgga a
//
LOCUS       DASWAV010000041         4665 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U2665
            k141_82688, whole genome shotgun sequence.
ACCESSION   DASWAV010000041 DASWAV010000000
VERSION     DASWAV010000041.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960316
            Sequence Read Archive: SRR10260007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 4665)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4665)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:47:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,929
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4665
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_82688"
                     /isolate="SMAG_U2665"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.36444 N 132.31889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1230)
                     /locus_tag="VF958_00885"
     CDS             complement(<1..1230)
                     /locus_tag="VF958_00885"
                     /inference="COORDINATES: protein motif:HMM:NF013647.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033179 - proton-transporting V-type
                     ATPase, V0 domain [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_function: GO:0046961 - proton-transporting ATPase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATPase 116kDa subunit family protein"
                     /protein_id="HEX9147690.1"
                     /translation="MLRPVPMVHLRIQVGNRDAAAVTRRIAAEGVLHLVDLAHGRVAA
                     GVEPPGVRDLAAGFRDLTRRIERLSQRLDAVLPELAGTLEGREAAELAAERQEIESRL
                     APIEAELETIWKKREEERERAARAKENLERVRRLAAAELDIRRLAPLSRVVLRLGIGD
                     PEALATIASLLSPAPFAVVPLEGDGGLAAIAVTGADRERLDAALRVVPFTPFAIPGDP
                     ASLDTAVIEREGRDAGERLRELSESLDEVKIRTLAEIAEIWRRAQVGLMLLEAQMHFA
                     ASGRFVVISGWVPEESADRLKRAILEKTEGRAIIEIDRPEDLPEVSSGALRVPILHRN
                     PILLRPFQKLVQLYGTPSYREIQPTAFFAVSFLAMFGLMFGDVGQGLVLCVAGYCLFR
                     YIPRYLDYGILLMEGGAA"
     gene            complement(1232..2281)
                     /locus_tag="VF958_00890"
     CDS             complement(1232..2281)
                     /locus_tag="VF958_00890"
                     /inference="COORDINATES: protein motif:HMM:NF014094.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATPase subunit"
                     /protein_id="HEX9147691.1"
                     /translation="MIAAFRLAFGNARMRARKSRLRTASAARLLGSAPGVAAFADSLG
                     LPPTNEQAELDRILFDRLLDDYRRILDDYSAARPLVHALLARHEVENLKLGWRALARG
                     LPPSRWIRLWRRFGALETLSLETFRDASSLAEAVRRASSTPWGRIVRETLEAHEHDPA
                     AAELALDVFAFARVAEEAEKLPRREAIARRLVLSLVSERDLDAFRRATAFSGVSPELA
                     GAATVRLSRDLPEETLATLAGWTEGALPFDLRLPAGIIPRSRAIHGWDALRMTIRRER
                     VAACRRAFAGPPFRLAPAIAFLLLREAETRALTALASVLPSLPGRGVGGEGRDHATAP
                     PSALERVFAASLLGD"
     gene            complement(2278..2607)
                     /locus_tag="VF958_00895"
     CDS             complement(2278..2607)
                     /locus_tag="VF958_00895"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="produces ATP from ADP in the presence of a proton
                     gradient across the membrane; the function of the H
                     subunit is unknown; Derived by automated computational
                     analysis using gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit H"
                     /protein_id="HEX9147692.1"
                     /translation="MKQDLETLICADEESRARVDAAREDARQRLEAARAERDRHLGER
                     KAEAEAMLAREVEAVREEGERLLEDRRRRREELLRVRRAEADARLERATQAYEQILRD
                     GPPRGAA"
     gene            complement(2604..3119)
                     /locus_tag="VF958_00900"
     CDS             complement(2604..3119)
                     /locus_tag="VF958_00900"
                     /EC_number="2.7.1.25"
                     /inference="COORDINATES: protein motif:HMM:NF013730.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylyl-sulfate kinase"
                     /protein_id="HEX9147693.1"
                     /translation="MSWAVWLTGRPAAGKTTLARALNELAASRGTPLVHLESDCLRRI
                     LTPRPTYEPEERERFYEIIADLAALLAAQGFPVIIDATAPRRVHRDRARRQIPRFLEV
                     LVDTPLEVCERRDPKGLYAASRRGRAPHLPGAGEPYEEPARPDLVVSGAAPPDEAVGA
                     LWRVLHGRGFL"
     gene            complement(3116..4222)
                     /locus_tag="VF958_00905"
     CDS             complement(3116..4222)
                     /locus_tag="VF958_00905"
                     /inference="COORDINATES: protein motif:HMM:NF013775.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HEX9147694.1"
                     /translation="MTSLSLEPPEDRFPGLREHLAAKFGPAAGLARIDPLATAPGAKG
                     GGYGVPQLVRWRSSDGDHRYVLETVRPGGFGHEDRADRASLVLRALDDYSELPRHVRA
                     VDAGAFSRSGAAVGLAGSEEFFLLTDFVEGEPYARDLERIAARGRLEPLDRDRALALA
                     EYLAAIHREPVVHETWYRRRLRDLAGSGECIAGIADSYPEGDFPGPAILERVEALALH
                     WRYRLRDRTERLRRIHGDFHPWNILFRQDSDFTVLDRSRGRFGDPADDVAALVVNYLF
                     FALRQHGRFEGPFAELFHLFWNRYRLSSGDASLADVIAPHFAFRTLVLANPVWYPSES
                     PATRRALFRFLFATLEADSFDPEGLEALLGEEES"
BASE COUNT          751 a         1605 c         1642 g          667 t
ORIGIN      
        1 ggcggctccc ccctccatca aaagaattcc gtagtcgagg taacgcggga tgtaacggaa
       61 gagacagtag cccgcgacgc aaagcacgag accctgcccg acgtcgccga acatcaggcc
      121 gaacatcgcg agaaagctca ccgcgaagaa cgcggtcggc tggatttccc ggtaggaagg
      181 tgtcccgtag agctgcacga gcttctggaa agggcgcagc aggatcgggt tgcgatggag
      241 gatggggacg cgaagcgcgc ccgaggagac ttccgggaga tcctccggcc gatcgatctc
      301 gatgatggcg cggccttccg tcttctccag gatcgcgcgc ttcaatcggt ccgcgctttc
      361 ctcgggcacc cagcccgaga tgacgacgaa tcgtccggac gcggcaaagt gcatctgggc
      421 ctcgaggagc atcaggccga cctgcgcgcg gcgccagatc tcggcgatct ccgcgagcgt
      481 gcggatcttc acctcgtcga gggactccga gagctcgcgg aggcgttcgc cggcgtcgcg
      541 tccctcgcgc tcgatcacgg ctgtgtccag ggaagccgga tcccccggga tcgcgaacgg
      601 cgtgaagggc acgacacgca gcgccgcgtc aagccgctcc cggtcggccc ccgtcacggc
      661 gatcgccgcg aggccgccgt ccccctcgag cggcacgacc gcgaacggcg ccggagaaag
      721 gagggatgcg atcgtcgcga gcgcttcggg atctccgatt ccgagacgca gaacgacgcg
      781 cgagagcgga gcgagccggc gaatgtcgag ttccgccgcc gcgaggcggc gcacgcgttc
      841 gagattttcc ttcgcgcggg cggcccgctc tctctcctct tcgcgcttct tccagatcgt
      901 ctcgagctcc gcctcgatcg gcgcgagtct cgactcgatc tcctggcgtt cggccgcgag
      961 ctccgcggcc tcgcggcctt ccagcgtgcc ggcgagctcc gggagaacgg cgtcgaggcg
     1021 ctgcgacagc cgctcgatcc ggcgtgtcag atcgcgaaat ccggccgcca gatcccgaac
     1081 tcccggcggt tcgacgcccg cggccacgcg tccgtgcgcg aggtcgacca agtgcagcac
     1141 tccttcggcg gcgatgcgcc gggtgaccgc ggccgcgtcg cggttgccga cctggattcg
     1201 aaggtggacc atcgggaccg gtcgcagcac gtcagtctcc caggagggaa gcggcgaaaa
     1261 cgcgttcgag agcggaggga ggcgccgtgg cgtgatcacg cccctcgcct ccgacccccc
     1321 tgccggggag agaggggagg acggacgcga gcgctgtcag ggctcgcgtc tccgcctccc
     1381 gcaggaggag gaacgcgatc gccggggcga ggcgaaacgg cggccccgcg aacgcccggc
     1441 ggcaggccgc gacccgctcg cgccggatcg tcatccgaag cgcgtcccag ccgtgaatcg
     1501 cccggcttcg cgggatgatt cccgcgggaa gccggagatc gaacggcagg gccccctcgg
     1561 tccagccggc gagcgtcgcg agcgtctctt ccggaagatc gcgagagagc cgcaccgtcg
     1621 ccgcgccggc gagctcgggt gagacaccgg agaacgccgt cgcgcgccgg aaggcgtcga
     1681 ggtcccgttc agagacgagc gagagaacga gtcgtcgcgc aatcgcctcc cgccggggaa
     1741 gcttctccgc ttcctccgcg actcgcgcga atgcgaacac gtcgagcgcg agctcggccg
     1801 ccgcggggtc gtgttcgtgt gcctccagtg tttcgcggac gatgcggccc catggggtcg
     1861 agctcgcgcg gcgaactgct tccgcgagag acgaggcatc ccggaatgtt tcgagtgaca
     1921 gcgtctcgag cgcgccaaag cgccgccaga gccggatcca ccggctcggc ggcaggccac
     1981 gagcgagcgc gcgccatccg agtttcagat tttcgacttc gtgcctcgcg agcagggcgt
     2041 ggacgagcgg ccgcgccgcg gagtagtcgt cgaggattcg ccggtagtcg tcaagcagcc
     2101 ggtcgaagag gatacggtcg agctcggcct gctcgtttgt cgggggcagt cccagcgagt
     2161 ctgcgaacgc cgcgacgccg ggggccgagc cgaggagccg cgctgccgac gccgtgcgga
     2221 gtcgcgactt gcgcgctctc atccgggcgt tgccgaaggc gagccggaat gcggcgatca
     2281 cgccgcgccc cggggcggtc cgtctcggag aatctgttcg tacgcctgcg tcgcgcgctc
     2341 gagccgcgcg tcggcctccg cgcgccgcac tcgaagcagc tcctcgcgac gacgccgtcg
     2401 gtcttccagg agcctctctc cctcctcgcg gaccgcttcg acctcgcgag cgagcatcgc
     2461 ttcggcttcc gctttcctct cgccgagatg tcggtcgcgc tccgcccggg ccgcctcgag
     2521 cctctggcgc gcgtcctcgc gcgcggcgtc cacgcgcgcc cgggactcct cgtcggcgca
     2581 gatcagcgtc tccaggtctt gtttcacagg aagccgcggc cgtggagcac ccgccagagc
     2641 gctccgaccg cctcgtcggg aggagccgcg cccgacacga cgaggtccgg ccgcgcgggt
     2701 tcctcgtagg gttctccggc tcccggaagg tgcggcgccc gaccccgacg ggatgccgcg
     2761 tacaggcctt tcggatcgcg ccgttcgcag acctcgaggg gcgtgtccac gaggacctcg
     2821 agaaaccgcg ggatctgacg ccgggctcgg tcacgatgga ctcgccgggg cgcggtggcg
     2881 tcgatgatga ccggaaatcc ctgcgcggcg aggagggcgg ccagatcggc gatgatctcg
     2941 tagaagcgct cccgctcttc gggctcgtag gtcggccggg gcgtgagaat ccggcgcagg
     3001 cagtccgact cgaggtggac gagcggagtc ccgcgggacg cagcgagctc gttcagcgcc
     3061 cgcgcgaggg tcgtcttccc cgcggcgggt cttcccgtga gccagacggc ccagctcacg
     3121 actcctcttc gccgagcagc gcttccaggc cctccggatc gaagctatcg gcttcgagcg
     3181 tcgcgaacag gaaacggaag agagcgcgcc gcgtggccgg gctctccgag ggataccaga
     3241 cggggttcgc gagcaccagc gtgcggaacg cgaagtgcgg cgcgatgacg tccgcgaggg
     3301 acgcgtcgcc cgaggagagc cgatagcggt tccagaagag atggaacagc tccgcgaacg
     3361 ggccctcaaa acggccgtgt tgccggagag cgaagaagag atagttgacg acgagcgccg
     3421 cgacgtcgtc ggccggatcg ccgaagcggc cccggcttcg gtcgaggacg gtgaaatccg
     3481 agtcctgccg gaacaggatg ttccaggggt ggaagtcgcc gtgaatccgg cggagccgct
     3541 ccgttcggtc gcgcagccgg taccgccagt ggagggcgag cgcctccacc cgctcgagga
     3601 tcgccgggcc gggaaagtcc ccttccggat acgagtccgc gattcccgcg atgcactcgc
     3661 cggaacctgc gagatcgcgg agacgccggc gataccacgt ctcgtgaacg accggctcac
     3721 gatgaattgc ggcgagatat tcggcgagcg cgagcgcccg gtcgcggtcg agcggctcga
     3781 gccgaccgcg cgcggcgatg cgttcgaggt cgcgcgcgta gggctctcct tccacgaaat
     3841 cggtcagcag gaagaattct tcgctgccgg cgagcccgac cgccgcgccc gatctcgaga
     3901 atgctccggc gtccacggcg cgcacgtgcc gcggcaactc gctgtagtca tcgagcgcgc
     3961 ggagaacgag agacgcccgg tccgcccgat cttcgtgtcc gaagccgccc ggccggacgg
     4021 tctcgagtac gtagcggtga tcgccgtcgg acgatcgcca ccggacgagc tgcggcacgc
     4081 cgtagccgcc ccctttggct cccggtgcgg tcgcgagggg gtcgatccgc gcgaggccgg
     4141 cggccgggcc gaatttcgcg gcgaggtgtt cgcgaagacc cggaaagcgg tcctccggag
     4201 gctccaggct gaggctggtc atgctcgtcg cctcggggag aaggctaggt ccggactgcc
     4261 cgcaaaccct gatgaggaca catcaaggaa cgcgcggacc tctgatccgt cgtcagctcg
     4321 aggttcgatt cccgccacgc acagaacgtc gggaccgtgg aaaatcgatc tcgctcacga
     4381 cctgcgcggg cgaacgatcc gactgcggag tccgcaggtc gccgacacct ccgatctgta
     4441 caggccgaca cctccgatct gtacagtcag gtgcaggcgc actggagcgt cgagggtccg
     4501 gactctttcc tgtgcgtcaa gtccctcgtc gcgcacttcg acgcgccggc gtcctcgcgc
     4561 ctgcgatcga cacgaccacg gaaagagagg accgtcctcg ccaaacagac ccgtcttcac
     4621 gggcttctcg tatctaatgc acccgcatca agcgggaaga acggg
//
LOCUS       DASWAV010000042         3971 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U2665
            k141_443233, whole genome shotgun sequence.
ACCESSION   DASWAV010000042 DASWAV010000000
VERSION     DASWAV010000042.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960316
            Sequence Read Archive: SRR10260007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 3971)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3971)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:47:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,929
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3971
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_443233"
                     /isolate="SMAG_U2665"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.36444 N 132.31889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..990
                     /locus_tag="VF958_00910"
     CDS             <1..990
                     /locus_tag="VF958_00910"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose/GDP-mannose dehydrogenase family
                     protein"
                     /protein_id="HEX9147695.1"
                     /translation="EAIAQYMNGYKVVVTKSTVPTGTGRMIEQILKSNDGEREFSVVS
                     NPEFLREGSAISDFLRPDRIVIGASDARAIEVMNEIYSPLFLSETPFVITDVASAELI
                     KYASNGFLAVKISFINEIARLCEVMGADVHDVARGMGLDRRIGARFLLAGPGFGGSCF
                     PKDTAAAIDLARQHGERFRIIEAAVCVNDETKKRMIEKITRVSGPLAGRTAAVLGLSF
                     KPETDDIRESPALSVISDMVAAGASVRAFDPAAMSNARSVFPDLYYASDVYDCARGAD
                     VLVLATEWNEFRVLDFGKLERLMKSKTFVDLRNVYEPRELRAAGWTYAGVGRP"
     gene            1016..1960
                     /locus_tag="VF958_00915"
     CDS             1016..1960
                     /locus_tag="VF958_00915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012257102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucuronic acid decarboxylase family
                     protein"
                     /protein_id="HEX9147696.1"
                     /translation="MRILVTGAAGFLGSHLCDRLLAEGHAVVAMDNFVTGNPRNVAHL
                     KDDRRFHFVEHNVTKFISVEGEIDAVLHFASPASPIDYLELPIQTLKVGSLGTHNALG
                     LALAKGARFLLASTSEVYGDPLVHPQPETYWGNVNPIGPRGVYDEAKRFAEAMTMAYH
                     RTHGVPTRIVRIFNTYGERMRLNDGRVVPALIGQALAGEPMTVFGDGSQTRSFCYVSD
                     LIDGIYRLLLSEESEPVNIGNPSELSVLEFARTIRRLTGTSSEIVFRPLPVDDPKVRQ
                     PDITRARTRLGWEPTVPLEEGLMRTIGYFRSVLARKED"
     gene            complement(1957..2190)
                     /locus_tag="VF958_00920"
     CDS             complement(1957..2190)
                     /locus_tag="VF958_00920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009959880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HEX9147697.1"
                     /translation="MADPVDKLPENASGRYYVDSQCIDCDVCRVTAPANFQRDGEKGY
                     SYVYSQPASPEEEAQTREAMDSCPVEAIGDDGD"
     gene            2287..2664
                     /locus_tag="VF958_00925"
     CDS             2287..2664
                     /locus_tag="VF958_00925"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HEX9147698.1"
                     /translation="MPGRVWFVSWLGIAVLAVGCSEEALPEADARGGDVAAGRVIFER
                     KCARCHNGNGDGQTPVAGRFPYANLVDGVWRSDGTAEAIERQIRRGRDPMPSFEKKLT
                     DEQIRQTVEYVLELTGGKRESSR"
     gene            2661..3302
                     /locus_tag="VF958_00930"
     CDS             2661..3302
                     /locus_tag="VF958_00930"
                     /inference="COORDINATES: protein motif:HMM:NF014269.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LON peptidase substrate-binding
                     domain-containing protein"
                     /protein_id="HEX9147699.1"
                     /translation="MTRRIPLFPLPGVVLLPGTLLPLHIFEPRYRAMVADALSGENTI
                     GMAMLKPGSERAGAVPEIFPIGGAGRIIASERLSDGRYDIVLQGVFRYRVLDESPPSP
                     YRVARVEEIESIPFRTDAEASRVSAEARSLFSEISGAMSLPPLPEETLSPERLGSELA
                     LRLRYEPSELQELLELDSLPARLDTLTTRMREWQNRIRLLSPFRRTGLDPSKN"
     gene            complement(3351..>3971)
                     /locus_tag="VF958_00935"
     CDS             complement(3351..>3971)
                     /locus_tag="VF958_00935"
                     /inference="COORDINATES: protein motif:HMM:NF012234.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEX9147700.1"
                     /translation="TEWNLPLFRLDMNAIFGTDNPEATFLKALRSVEAVSPAVLWIDE
                     IEMGVGGYREGQDASMSRIFSGFLTWMQEKSALVFVAATANRIHLLPAEIIRKGRFDQ
                     VFFVDLPSEEERKQIFDIHLTKNRCDVSRLDLVFLAKATAGWNGAEIEQAVLSAVVDA
                     YAEKREVTEDDLYRMITATVPLSVTMEEQIKSIRSWAHERALNASR"
BASE COUNT          651 a         1317 c         1310 g          693 t
ORIGIN      
        1 gaggcgatcg cgcagtacat gaacggctac aaggtcgtgg tgacgaagtc caccgtaccg
       61 accggcacgg ggcggatgat cgagcagata ttgaagtcga acgatgggga gcgggagttc
      121 tccgtcgtgt cgaacccgga gttcctccgc gagggttcgg cgatctccga ttttctgcgc
      181 cccgaccgca tcgtcatcgg agcctcggat gcacgcgcga tcgaggtgat gaacgagatc
      241 tacagcccgc tctttctctc ggagaccccg ttcgtcatca ccgacgtcgc gtcggccgag
      301 ttgatcaagt acgcctcgaa cgggttcctc gcggtcaaga tttcgttcat caacgagatc
      361 gcccgactct gcgaggtcat gggcgccgac gtccacgacg tcgctcgggg gatggggctc
      421 gatcgccgga tcggcgccag gttcctcctc gccggcccgg gtttcggcgg atcctgtttc
      481 ccgaaggaca cggcggcggc gatcgacctc gcgcgccagc acggagagcg cttccgcatc
      541 atcgaggcgg cagtctgcgt gaacgacgag acgaagaagc gaatgattga aaagatcacg
      601 cgggtctccg ggccgctcgc ggggcgcacg gcggcggtcc tggggctctc gttcaaaccg
      661 gagaccgacg acatccgcga atcgccggcg ctttcggtga tttccgacat ggtcgccgcc
      721 ggcgcctccg tgcgcgcgtt cgaccccgcc gcaatgtcga acgcccgctc ggtctttccg
      781 gacctctact acgcgagcga cgtctacgac tgcgcgcgcg gggccgacgt cctcgtcctg
      841 gcgaccgagt ggaacgagtt ccgcgttctc gactttggaa agctcgagcg cctgatgaag
      901 tcgaagacgt tcgtcgacct gcgaaacgtc tacgagccga gggagctccg cgccgcgggc
      961 tggacgtacg cgggagtggg aaggccgtga ggccttcgcg gcctcggggg ccccggtgag
     1021 gattctggtc acgggggccg ccggcttcct gggcagccac ctctgcgatc ggctgctcgc
     1081 ggaaggtcac gcggtcgtgg ccatggacaa cttcgtcacc ggcaacccgc gaaacgtcgc
     1141 gcacctgaag gacgaccggc gatttcactt cgtcgaacac aacgtcacga agttcatctc
     1201 cgtggaggga gagatcgacg cggtccttca cttcgcctcc cccgcttcgc cgatcgatta
     1261 cctcgaactc ccgatccaga cgctgaaggt cgggtcgctc ggcacgcaca acgccctggg
     1321 cctcgcgctc gccaagggcg cgcgtttcct gctcgcttcc acctccgagg tctacgggga
     1381 cccgctcgtt cacccgcagc ccgagaccta ctggggcaac gtgaacccca tcgggccgcg
     1441 cggcgtctac gacgaggcga aacgcttcgc cgaggcgatg acgatggctt atcaccgcac
     1501 gcacggcgtt ccgacccgga tcgtccggat cttcaacacc tacggggagc ggatgcgctt
     1561 gaacgacggc cgtgtcgtcc ccgcgctcat cggccaggcg ctcgccggcg agccgatgac
     1621 cgtcttcggc gacggctcgc agacgcgctc gttctgctac gtgtccgacc tgatcgacgg
     1681 gatctaccgg ctccttctct ccgaggagtc cgagccggtc aacatcggca acccgagcga
     1741 gctttcggtg ctggagttcg cccggacgat ccggcggctg accggtacgt cgagcgaaat
     1801 cgtgtttcga ccgctcccgg tggacgatcc gaaggttcgt cagccggaca tcacgagggc
     1861 ccgcacgcga ctgggatggg agccgacggt tccgctcgaa gaagggctga tgcggacgat
     1921 cgggtacttc cggtcggtcc tggcccggaa ggaagactag tcgccgtcgt ccccgatcgc
     1981 ctcgaccgga caggaatcca tcgcctcccg ggtctgcgcc tcttcctcgg ggctggccgg
     2041 ctggctgtag acgtacgagt accccttctc cccgtcgcgc tggaagttgg cgggagcggt
     2101 gacgcgacac acgtcgcaat cgatgcactg ggagtcgacg tagtaccgcc cggaggcatt
     2161 ttcgggaagc ttgtccaccg ggtcggccac gggagggtgc gacggtagcg actgccccga
     2221 cgggctgtca actcaggaat atctgaacgg gagcggcact ctccatccgg aggctagact
     2281 tcgcgcgtgc ccggaagagt ctggttcgtc tcgtggcttg gcatcgccgt gctcgcggtc
     2341 gggtgtagcg aggaggccct ccccgaggcc gatgcgcgcg gtggagacgt cgccgccgga
     2401 cgcgtcatct tcgagcgcaa gtgcgcgcga tgccacaacg gcaacggcga cggtcagact
     2461 cccgtcgcgg gaagattccc gtacgcgaac ctcgtcgacg gcgtgtggcg gtccgacggg
     2521 accgccgagg cgatcgagcg ccagatccgg cgaggacgcg atccgatgcc gtccttcgaa
     2581 aagaaactga cggacgagca gatccgccag acggtcgagt atgtgctgga gctgaccggc
     2641 gggaagcggg agtcgagtcg gtgacccgtc ggatccccct ctttccgctc ccgggggtcg
     2701 ttctccttcc aggaacgctc ctgccgctgc acattttcga gccgcggtac cgggcgatgg
     2761 tcgccgacgc cctgtcgggc gagaacacga tcggcatggc gatgctgaag cccggttcgg
     2821 agcgcgccgg ggcggtcccg gagatcttcc cgatcggagg cgccggccgc atcatcgcgt
     2881 ccgagagact ctccgacggg cgctacgaca tcgtcctcca gggagtgttt cggtaccgcg
     2941 tgctcgacga gtcgcccccc tcgccctacc gcgtcgcgcg ggtcgaggag atcgaatcga
     3001 tcccgttccg gacggatgcc gaagcgagtc gcgtctccgc tgaagcgcgc tctctcttct
     3061 ccgagatctc cggcgcaatg tccctgccgc ctctgccgga ggaaaccctt tcccccgagc
     3121 gcctgggctc ggagctcgcg ctacgcctgc gatacgagcc ctcggagctc caggaactcc
     3181 tcgagctgga ctcgcttcct gcgcggttgg acacgctcac gacccggatg cgggaatggc
     3241 agaatcggat ccggttgctt tcgccgtttc ggcgaaccgg gctcgatccc tcgaagaact
     3301 gacccgttgg gtcgtcggga gccaggcgga ggatcgccgg cgagattccc tcagcgagac
     3361 gcgttcaacg ctctttcgtg cgcccagctg cggatcgact tgatctgctc ctccatcgtc
     3421 accgacagag gcacggtcgc ggtgatcatc cggtagagat cgtcctcggt gacctcgcgc
     3481 ttctccgcgt acgcgtcgac gaccgccgac aagaccgcct gttcgatttc cgcgccgttc
     3541 cacccggccg tcgccttggc gagaaagacc aggtcgaggc gcgagacgtc gcagcggttc
     3601 ttcgtcaggt ggatgtcgaa gatctgcttg cgctcttcct cgctcggcag gtcgacgaag
     3661 aagacctggt cgaaccgccc tttccggatg atctcggccg gcaggaggtg gattcggttg
     3721 gcggtcgcgg cgacgaagac gagggcgctc ttctcctgca tccaggtcag gaacccggag
     3781 aagatccggg acatcgaagc gtcctgcccc tcgcggtaac cgccgactcc catctcgatc
     3841 tcgtcgatcc agaggaccgc gggtgagacg gcctcgacgg accgcaacgc cttcagaaac
     3901 gtggcctccg ggttgtcggt tccgaagatc gcgttcatgt cgagccggaa gagcggcagg
     3961 ttccattcgg t
//
LOCUS       DASWAV010000043         4006 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U2665
            k141_522278, whole genome shotgun sequence.
ACCESSION   DASWAV010000043 DASWAV010000000
VERSION     DASWAV010000043.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960316
            Sequence Read Archive: SRR10260007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 4006)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4006)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:47:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,929
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4006
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_522278"
                     /isolate="SMAG_U2665"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.36444 N 132.31889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1466
                     /locus_tag="VF958_00940"
     CDS             <1..1466
                     /locus_tag="VF958_00940"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011320775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015662 - P-type ion transporter activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0043169 - cation binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HEX9147701.1"
                     /translation="EQAIRSLDRRLQAGAESRYSNGEIVRGYPLSPDLLAMSNAWRAS
                     RESGRENELVIAAKGAPETIAELCRLSATETEGLTRAVHALAEDGLRVLAVARTTFDL
                     LELPATQRGFHFELLGLLGLADPVRPEVPAAIRECHAAGIRVVMITGDYPVTAVQIAR
                     AIGLSRGTDVSIATGSELDGMDESTLREQIESIDVFARVRPEQKLAIVRALQGNGEVV
                     AMTGDGVNDAPALKAADMGIAMGRRGTDVAREAASLVLLDDDFASIVAAARMGRRIFD
                     NLRNASAYIFAIHVPIAGMSLLPIVFGWPLVLMPVHIVFLELIIDPACSLVFEAEPEE
                     DDVMSRPPRRPDESLFSKRIVTLGLLQGAGVLVIVLAMFAVAFRRGQGAEDARALAFT
                     TLIVANLALILTNRSWTRTIIQSFRTKNGALLWVLGGAVGLLALLLAVPGVRDLFGFS
                     KLHPVDLALCLAAGAFSIAWFELFKMFRARKSLSAAG"
     gene            1556..3316
                     /locus_tag="VF958_00945"
     CDS             1556..3316
                     /locus_tag="VF958_00945"
                     /inference="COORDINATES: protein motif:HMM:NF017727.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF542 domain-containing protein"
                     /protein_id="HEX9147702.1"
                     /translation="MNASSIDPQVPIRVLLARMPHLSSALAARGLDSCCGGEHPLAEA
                     CAARGMPLDDVVADLESAHRAAEAHSIVPPTMSVREIRRRFPATAPVFERYGLGDCGG
                     PDGPEEPVAWFATVHRLPLDEFLRDVREAAVRDVAAAPPAPESSARPFAPHFILGSLF
                     LTLTLGATTGMINLLRIAAGGDVPISHRQIHGHTQVLGFAALFLMGIAYHALPRILGI
                     GARTPASSRIAFWLMFGGVILRNAGQPLQFYAVGRVLSFVSAAMELAAGLLFARFVFA
                     LLAEMRVGKYDRRDPILRFLRIGTVFFVGALVLVGAQGIWLAGNRDTSLPSALTEPFY
                     FLALYGFLLAWIYGFGNRVVSLFLGVGPARRGTPEIAMALQAVAAPLYCVGWLPMVPP
                     AAGLLLRDGGLVLVALSAVVYLFGNGFLWRKSELPALRAAGSPTFAIRAAFGSLGLWA
                     ALELAAVVLSRTTAIPSQNPWWADAGRHVFTIGFLTLLIVGMSMRILPVFSGKTLWSP
                     ALARATYGLLLIGTAMRLLQYPAAFRPLFYQIGSYMGIPVVLALVLFTFNLFRTMRPN
                     RARPDKKRPTFSSSLPVHGV"
     gene            3755..>4006
                     /locus_tag="VF958_00950"
     CDS             3755..>4006
                     /locus_tag="VF958_00950"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HEX9147703.1"
                     /translation="MPVRPPIPFGAVPALASLTREDRDALEPLCELRAYEKGARIFEE
                     GDPADDIYFLFLGRVKIVKMAGDRDLILEILGPGEPVGAV"
BASE COUNT          536 a         1451 c         1283 g          736 t
ORIGIN      
        1 tggagcaggc gatccgctct ctcgaccggc gcctgcaggc gggtgcggaa agtcgttatt
       61 cgaacgggga gatcgtccgc ggctatcccc tctccccgga cctcctggcg atgtcgaacg
      121 cctggagggc gtcgcgagag agcggacgcg agaacgaact cgtgatcgcc gcaaagggag
      181 cccccgagac gatcgccgag ctctgccgcc tctcggcgac cgaaacggag gggctcacgc
      241 gtgccgtcca cgctctcgcg gaagacgggc tgcgcgtcct cgccgtcgcc cggacgacct
      301 tcgatcttct ggagcttccc gcgacgcaac gcggattcca cttcgagctc ctcgggctgc
      361 tcgggctggc cgatccggtc cgtcccgaag tccccgccgc gatccgcgag tgccatgctg
      421 ccggcatccg ggtcgtcatg atcacgggcg actaccccgt cacggccgtc cagatcgcgc
      481 gcgccatcgg cctctcccgg ggaaccgacg tctcgatcgc gaccggctcc gagctcgacg
      541 ggatggacga atccactctt cgcgagcaga tcgagtcgat cgatgtcttt gcccgcgtga
      601 ggccggagca gaagctcgca atcgtccgtg cactgcaggg caatggcgaa gtcgtcgcga
      661 tgaccggcga cggagtcaac gacgcgcccg cgctaaaagc ggccgacatg ggcatcgcga
      721 tgggacgacg gggaaccgac gtcgcccggg aagccgcctc tctcgtcctg ctcgacgatg
      781 acttcgcctc gatcgtcgcc gccgcgcgga tgggacgacg gatcttcgac aacctgcgca
      841 acgcgtccgc ctacatcttc gccatccatg tcccgatcgc cgggatgtcg cttctaccga
      901 tcgtgttcgg ctggccgctc gtcctgatgc cggtgcacat cgtctttctc gagctcatca
      961 tcgatcccgc ctgctcgctc gtgttcgagg ccgagccgga ggaggacgac gtgatgagcc
     1021 gcccaccgcg ccgccccgac gagtctctgt tctcgaagag gatcgtgacc ctcggactcc
     1081 tccagggcgc gggggtcctg gtgatcgtcc tggccatgtt cgcggtcgca ttccggcgcg
     1141 gccagggcgc cgaggacgcc cgggcgctcg cgttcacgac gctcatcgtc gccaacctcg
     1201 ccctgattct cacgaaccgc agctggacgc ggacgatcat ccagtcgttc cgcacgaaga
     1261 acggcgccct cctctgggtc ctcggtgggg ccgtgggtct cctggctctc cttctcgcgg
     1321 tgcccggcgt ccgcgacctc ttcggcttct cgaagctcca cccggtggat ctcgcgctct
     1381 gcctcgcggc gggagccttc agcatcgcct ggttcgagtt gttcaagatg ttccgcgccc
     1441 ggaagtcgct ctccgcggcg ggctgagccg tggcgcgcga ggcggtcacc gcgaagcggc
     1501 gccgcgtatg accggcgtca tggcgtcggt gggcagccgg gcagatcgtg acgtcatgaa
     1561 cgcctcctca atcgacccgc aggttccgat ccgggtgctg ctcgcacgga tgccgcacct
     1621 ctcctcggcg ctcgcggcgc gggggctcga ctcctgctgc ggcggagagc atccgctcgc
     1681 ggaggcgtgc gccgcgcgcg ggatgccgct cgacgacgtc gtggccgacc tcgagtccgc
     1741 tcatcgcgcg gccgaggccc actcgatcgt gccgccgacc atgtctgtcc gcgagatccg
     1801 gcgtcgcttc cccgcgaccg cgccggtctt cgaacgctac ggcctcggcg actgcggcgg
     1861 accggacggg ccggaagagc ccgtcgcctg gttcgcgacc gtccatcgcc tgccgctcga
     1921 cgagttcctc cgcgacgtcc gggaagccgc cgtgcgcgac gtcgccgcgg cgccgccggc
     1981 gccggagagc tcggcccgcc cgttcgcgcc gcacttcatc ctcggctccc tcttcctgac
     2041 gctcacgctc ggcgcgacga cggggatgat caacctcctg cgaatcgccg ccggcggcga
     2101 cgttcccatc tcgcaccgcc agatccacgg tcacacgcag gtgctcgggt tcgcggcgct
     2161 cttcctgatg ggaatcgcgt accacgcgct gccgcgcatc ctgggcatcg gcgcccgaac
     2221 gcccgcgtct tcgcggatcg ccttctggct gatgttcggc ggagtgatcc tgcgcaacgc
     2281 cgggcagcca cttcagtttt atgccgtggg gcgagttctc tcgttcgtct ccgcagcaat
     2341 ggagctcgcc gccggtctgc tcttcgcgcg gttcgtgttc gcgctgctcg cagagatgag
     2401 agtgggaaag tacgaccgcc gcgatccgat tctccggttc ctccggatcg gaaccgtctt
     2461 cttcgtcggc gccctcgtcc tggttggcgc gcagggaatc tggctcgcgg gaaaccgcga
     2521 cacttcgttg ccgtcggcgc tcaccgagcc gttctacttc ctcgcgctct acgggttcct
     2581 gctcgcgtgg atctacggtt tcggcaatcg ggtcgtgtct ctcttcctgg gcgtcggccc
     2641 ggcccggcgg ggaacgccgg agatcgccat ggctttgcag gccgtcgcgg cgccgctcta
     2701 ctgcgtcggg tggctgccga tggtgccgcc cgccgccggc cttctccttc gcgacggtgg
     2761 cctcgttctc gtagcgctgt cggccgtcgt ctacctcttc gggaacggct tcctctggag
     2821 gaagtcggag ctccccgccc tgcgcgcggc gggatcgccc acgttcgcca tccgcgcggc
     2881 cttcggttcc ctcggtctgt gggcggcgct cgagctggct gcggtcgtcc tctcgcggac
     2941 gacggcgatc ccctcacaga acccgtggtg ggcggatgcg ggccggcacg tcttcacgat
     3001 cgggttcctg accctgctca tcgtcgggat gagcatgcgc atcctgccgg tcttctcggg
     3061 aaagacgctg tggtcgcccg cgctcgcccg cgcgacgtac gggctgctcc tgatcggtac
     3121 cgcgatgcga ctcctccagt accccgcggc gttccggccg ctcttctacc agatcgggtc
     3181 gtacatgggc attccggtgg tcctcgccct cgttctgttc acgttcaacc tgttccggac
     3241 gatgcgtccg aatcgggcgc gtcccgacaa gaaaagaccc acgttttcgt cgagcctgcc
     3301 ggtccacggc gtctgatgaa ttctcatcag agaggcacgc agcggatgta tgaatctcgc
     3361 cttcagggga gaacgtgacg cagacgcaaa attcgaacga gagctcgcga actcagcgaa
     3421 ctcggcgctc ttgcgctcgt ctgtcgattc gcggcctgcg acatctttcc cgacccgtct
     3481 tccccgcttc tccgggcccc tccctggccc ggacctccgc cggcgctctc gcggtgacat
     3541 gctccgctcc gccgggccgt tcggcttcga gacaggtcgg cttctcccca ttccacgggc
     3601 cggtccagac accgcacccc cctggcgcgt ccagatacgt ctgaccggcc cgtttcctta
     3661 acttgatcgc ggcgcgcgcg tagcgaacac gccgccgcag gcactcgggg gacggcgctg
     3721 actccaggag aaagccgctt cctataatcg cggcgtgccg gtgcgacctc ccatcccgtt
     3781 cggcgccgtt cccgctctcg cttcattgac ccgggaagat cgcgacgctc tcgagccgct
     3841 ctgcgaactt cgcgcctacg agaaaggcgc caggatcttc gaagagggag atcccgccga
     3901 cgacatctat ttcctgttcc tcggacgcgt gaagatcgtc aagatggcgg gcgatcggga
     3961 cctcatcctc gagatcctcg ggccgggcga gccggtcgga gccgtc
//
LOCUS       DASWAV010000044         1615 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U2665
            k141_443358, whole genome shotgun sequence.
ACCESSION   DASWAV010000044 DASWAV010000000
VERSION     DASWAV010000044.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960316
            Sequence Read Archive: SRR10260007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 1615)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1615)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:47:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,929
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1615
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_443358"
                     /isolate="SMAG_U2665"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.36444 N 132.31889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(96..467)
                     /locus_tag="VF958_00955"
     CDS             complement(96..467)
                     /locus_tag="VF958_00955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9147704.1"
                     /translation="MAASGSALGSILLTALLVGPISVPGTPSATVRAFYAFHFAHDMA
                     FTPESVRVKNRWLAPVLVDLCESYFRRPASSEEVPEIDGDPFTDSQEYPKRFRVGAAR
                     VSGEAARVPVTCIGRTDRRAT"
     gene            complement(470..856)
                     /locus_tag="VF958_00960"
     CDS             complement(470..856)
                     /locus_tag="VF958_00960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9147705.1"
                     /translation="MQVKTGLSYPPEANRWISLLFLLDLDSPDLRGRMSVIGRSRIEK
                     EDTVVVELRGKDGRLHRLEFDARRGSLRRISDEVPTLVGPLPEEYVLLDYRKVHGVRV
                     PFTMEWSRGDYHVVHRLTRVELMDSK"
     gene            complement(847..1458)
                     /locus_tag="VF958_00965"
     CDS             complement(847..1458)
                     /locus_tag="VF958_00965"
                     /inference="COORDINATES: protein motif:HMM:NF014346.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030077 - plasma membrane
                     light-harvesting complex [Evidence IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0019684 - photosynthesis, light reaction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="photosynthetic reaction center cytochrome c
                     subunit family protein"
                     /protein_id="HEX9147706.1"
                     /translation="MKERRGGGLGKATFPGWVVLSLPGLPAPARPAHDDPPVERTQKN
                     IRVLHGLPTSQLIPVMAVMANPLGVTCAHCHTSERESDDKPPKEAARRMIRMTLDANR
                     VFYEAKTVITCNTCHRGAIEPVGIPRIADAGWNRSRPAAPEPNGLPTVGDVLDRYVLY
                     LGGWERIRRIGARRLTGVVSRDSGRASAAGDFLIDQRSRTTCR"
BASE COUNT          294 a          529 c          529 g          263 t
ORIGIN      
        1 ccctcacttc gaaagcagct gtcggagagg cgggccctct tccgggctca cgtcggagat
       61 cagccatctt ccggcgaccc ggacgagttg gacggtcacg tcgcgcggcg atccgtgcga
      121 ccaatacagg tcacgggcac tcgcgcagct tcgcctgaga cgcgggccgc cccgacgcgg
      181 aaacgtttcg gatattcctg cgaatccgtg aacggatctc cgtcgatctc gggcacctcc
      241 tcggaggacg cgggcctgcg aaaataactc tcgcagaggt cgacgagaac gggcgcgagc
      301 caccggttct tcacacggac cgactccggc gtgaacgcca tgtcgtgggc gaagtggaag
      361 gcgtagaacg cccgcacggt cgccgacggc gtgccgggga cggaaatggg accgacgagg
      421 agcgccgtga gaaggattga tcccagggcc gaccccgagg ccgccattgc tacttcgagt
      481 ccatcagttc gactcgagtg agacggtgca cgacgtgata gtcgccgcgc gaccattcca
      541 tcgtgaacgg gacgcggact ccgtggacct tgcggtagtc gagcaggacg tactcctccg
      601 ggagcgggcc gacgagggtc ggcacctcgt ccgagatgcg ccgcagcgac ccgcggcgcg
      661 cgtcgaactc gaggcgatga agacgaccat cctttccccg cagctccacc acgacagtat
      721 cttccttctc gatccggctt cgaccgatca cgctcatcct tccccgaagg tcgggactgt
      781 ccaggtcgag aaggaagaga agcgagatcc agcgattggc ctcgggtggg taggagagac
      841 ctgtcttcac ctgcatgtcg ttcggcttct ctggtcgatc aggaaatctc cggcggcgct
      901 ggcccttccg ctgtcccgcg acacgacacc ggtgagtcgc cgcgcgccga tccgccggat
      961 ccgttcccat cccccgagat acaagacgta gcgatcgaga acgtctccca ccgtgggcag
     1021 gccgttcggt tccggagccg cgggccggct tcgattccaa ccggcgtccg cgatgcgagg
     1081 gatgccgacg ggctcgatgg ctccgcggtg acaggtgttg cacgtgatga ccgtctttgc
     1141 ctcgtagaag acgcggttcg cgtcgagcgt catgcggatc atccgccgcg ccgcctcctt
     1201 gggcggctta tcgtcgctct cccgttcgct cgtgtggcaa tgcgcgcacg tcacgcccag
     1261 gggattcgcc atcacggcca tcacggggat gagttgggaa gtcggaaggc cgtgcaggac
     1321 tcggatgttc ttctgggtcc gctcgaccgg cggatcgtcg tgcgccgggc gcgcaggtgc
     1381 cggcagaccc ggcagggaca gcacaaccca tcccgggaag gtggcctttc caaggccccc
     1441 accgcgacgc tccttcacga aatcaactcg ccgaacggag ggcctgtcga agagcgaagt
     1501 ggcccgccca gcaggtgatg ccgatggccg cgcaaaccgc gaaggcaacg ggatggtccc
     1561 gcgcgagaaa gtgaaaatcc tcgaggcgcc cttccgaaaa cgtgaactga atgct
//
LOCUS       DASWAV010000045         1696 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U2665
            k141_122318, whole genome shotgun sequence.
ACCESSION   DASWAV010000045 DASWAV010000000
VERSION     DASWAV010000045.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960316
            Sequence Read Archive: SRR10260007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 1696)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1696)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:47:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,929
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1696
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_122318"
                     /isolate="SMAG_U2665"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.36444 N 132.31889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..835
                     /gene="mobB"
                     /locus_tag="VF958_00970"
     CDS             <1..835
                     /gene="mobB"
                     /locus_tag="VF958_00970"
                     /inference="COORDINATES: protein motif:HMM:TIGR00176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="molybdopterin-guanine dinucleotide biosynthesis
                     protein B"
                     /protein_id="HEX9147707.1"
                     /translation="SFLPTRFRPMFEKHAGETCSGAIVRATDPGVFRPFETGLRIIET
                     ARNLAPSRVRWRTEPYEFDSRPAIDLLTGSARFREALDSGAGATGEASRHRDEERKFL
                     ARRSPCLLYPDHRPAILAFVGGHEAGKTTVLTDTIARLRARGLTVGAVKHTSRAFEDD
                     SVGKDSQRLGASGAKVWALMTPERATVRRGVEEFGLPDFVRRHFSDCDVVLVEGFKSL
                     PVPKIEVTRAGAARPPISDALARISDRPAADSVPTYRFDDPDGIVALVLRLTGLDRAQ
                     G"
     gene            845..1504
                     /locus_tag="VF958_00975"
     CDS             845..1504
                     /locus_tag="VF958_00975"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024811.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HEX9147708.1"
                     /translation="MFLVFDLDGTLIDGYEGIADALAFAMERLGRAPLGIEQVRGMVG
                     EGLERLIEKAIGAGLVSQGVKLFRERYAEIADEKTRLMPDVPALLERLAAAGHAMSVA
                     SNKPADFSRQILASKGIAAYFTSVAGPGPGVPPKPHPEMLRRLMREAGAARGDTVVVG
                     DMEIDAEFARAGGCRAVLVAGGSRSRTELAEVAADAHLSRLSELSDWVDRITRRPPGS
                     A"
     gene            1514..>1696
                     /locus_tag="VF958_00980"
     CDS             1514..>1696
                     /locus_tag="VF958_00980"
                     /inference="COORDINATES: protein motif:HMM:NF012607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HEX9147709.1"
                     /translation="MSRVVVAEELSPAGLEILRTAGHEVVELAGANRERLLAALGDAD
                     ALLVRSRTKVDAALLAA"
BASE COUNT          234 a          593 c          597 g          272 t
ORIGIN      
        1 ttccttcctg ccgacccggt ttcggcccat gttcgaaaaa cacgcggggg agacgtgcag
       61 cggcgcgatc gtgcgggcca ccgaccccgg ggtcttccgg ccgttcgaga ccggcctgcg
      121 gatcatcgag accgcccgga acctcgcccc ttcgcgcgtc cggtggagga ccgagcctta
      181 cgagttcgac tcgcggcccg ccatcgacct cctgaccggc tcggcgcgtt tccgggaagc
      241 gctcgactcc ggagccgggg ccacggggga agcgagccgc catcgggacg aggagagaaa
      301 gttcctcgcg cggcggagcc cgtgccttct ctatcccgac caccggccgg caatactcgc
      361 tttcgtcggt ggtcacgaag ccgggaagac gaccgttctc accgatacga tcgcccggct
      421 tcgggcccga gggcttaccg tgggtgcggt caagcacacg tcgcgagctt tcgaggacga
      481 ttcggtcggg aaggactcgc agcggctcgg agcttccggc gcgaaggtgt gggcgctcat
      541 gaccccggag cgggcgacgg tgcgtcgcgg cgtcgaggag ttcggccttc cggacttcgt
      601 ccgccgtcac ttctccgact gcgacgtcgt gctcgtcgaa ggcttcaagt cgctcccggt
      661 ccccaagatc gaagtgacgc gggccggcgc ggcgcggccg ccgatctctg acgcactcgc
      721 ccgaatatcc gatcggccgg cggcggactc ggtgccgacg taccgtttcg acgacccgga
      781 cgggatcgtc gcgctggtgc tgcggctcac cggcctcgac cgggcgcagg gctgatcgct
      841 ccgcgtgttc ctcgtcttcg atctcgacgg gaccctgatc gacggctacg agggcatcgc
      901 cgacgcgctc gccttcgcga tggagcggct cgggcgtgcg cccctcggaa tcgagcaagt
      961 tcgagggatg gtcggcgagg ggctcgagcg gctgatcgaa aaggcgatcg gggcgggcct
     1021 cgtttcgcag ggcgtgaagc tcttccgcga gcggtatgcc gagatcgcgg acgagaagac
     1081 gcggctcatg cccgacgtcc ccgcccttct cgagcggctc gcggcggccg gtcatgcgat
     1141 gtcggtcgcg tcgaacaagc cggccgattt ctcgcggcaa atcctcgcgt cgaaggggat
     1201 tgccgcctat ttcacgtcgg tcgccggtcc gggccccggc gttccgccga aaccccaccc
     1261 ggagatgctc cggcgcctca tgcgcgaagc gggagcggct cgcggcgata cggtcgtcgt
     1321 cggggacatg gagatcgacg ccgaattcgc gagggccggc ggctgccgcg ccgtgctcgt
     1381 ggccgggggc tcgcgctctc gaacagagct cgcggaggtc gctgcggatg ctcacctctc
     1441 ccgtctctcc gagctctccg actgggttga ccgcatcacg cggcgccccc ccggctcagc
     1501 ctagaatcgg ccgatgagcc gggtcgtcgt cgccgaagag ctttcccccg cgggtctcga
     1561 gatcctccgc acggcggggc acgaggtcgt cgagctcgcc ggcgcgaatc gcgagcgcct
     1621 cctcgccgcg ctcggtgatg cggacgcgct cctcgtgcga agccggacga aggtggacgc
     1681 tgcgctcctc gccgcc
//
LOCUS       DASWAV010000046         3866 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U2665
            k141_42088, whole genome shotgun sequence.
ACCESSION   DASWAV010000046 DASWAV010000000
VERSION     DASWAV010000046.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960316
            Sequence Read Archive: SRR10260007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 3866)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3866)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:47:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,929
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3866
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_42088"
                     /isolate="SMAG_U2665"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.36444 N 132.31889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..635)
                     /locus_tag="VF958_00985"
     CDS             complement(<1..635)
                     /locus_tag="VF958_00985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002625236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HEX9147710.1"
                     /translation="MRRRLAIAAAIVLSSSLTLLAAAPAAPKKAARVPKQYTIEQFFA
                     TTGVRDASFSSDEKRILFSSNQTGVYNVYSVPATGGAPTNLTSSATDTTYAVSFFPGD
                     DRVLYTRDQGGNELNHLYVREADGKERDLTPGDKLKADFDGWTGDRTAFWVETNERDP
                     KYFDLYRYDAKTYERALVYQNEGYQIGAISPDGKSVALVKTNGTSDSDVYL"
     gene            complement(632..1756)
                     /locus_tag="VF958_00990"
     CDS             complement(632..1756)
                     /locus_tag="VF958_00990"
                     /EC_number="1.3.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF013355.4"
                     /note="NADH-dependent; catalyzes the conversion of
                     pyrimidines to 5,6-dihydro compounds in pyrimidine
                     degradation; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent dihydropyrimidine dehydrogenase
                     subunit PreA"
                     /protein_id="HEX9147711.1"
                     /translation="MPDLSVKFTGIDFENPFLLSSAPPTESESNILRAFEAGWAGVVT
                     KTIGVHPVVNVKGPKTNFLRTDPETGHISMKKKPGTALHSSWNWELISDKPLDWWIGR
                     LARIKKGHPTKVLVASIMAGSGSDEEMRHWQTLAKACQEDGADALELNLSCPHMDRPD
                     MGSNIGKDDELISVVTQAVKEVAKVPVWAKLTPSTTDIVVEARGAFLGGADAISSSNT
                     FTSLPVIDPETLEFEMNVEGYVSTGGLGGPAILPLSLAKMAQMTGAFPDKEFSGIGGI
                     AEFAHALNYFLLGCGTVQVCTAAMLDHAVGPNVIRNLVAGMSEFLEKNAHRGWKNLED
                     FRGLRRNRIVPHSQIKRPDDSDYHGGYDNEAPAAAGRSIK"
     gene            complement(1773..3110)
                     /locus_tag="VF958_00995"
     CDS             complement(1773..3110)
                     /locus_tag="VF958_00995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013378161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HEX9147712.1"
                     /translation="MSTEIRQTRPVSDLSRTVREKHKEYLFPATIQYYQEPIVVTEAK
                     GLRVRDADGNEYLDFFGGILTVSVGHANEKVNTAVKAQVDRLSHISTLYPTLPVVELA
                     ERLARLAPGNLRQTYFTASGTEADETAVMMAELFTGRSELIALRHGYSGRSMLAQSLT
                     AHSSWRALPTKIAGVKHAPTPYCYRCPMHLEYPSCGLACAKDIEDIIRTTTTGRISAF
                     LAEPIQGVGGFITPPAEYFEAAVAIIRKYGGVFICDEVQTGFGRTGTKMWGIEHWGVE
                     PEIMTTAKGVANGLPLGVTICTPEIAASLKALTISTFGGNPVSCAAANATIQIIEEEN
                     LPDNAETMGRRLRGGLEELKRRFPKNLGDVRGMGLMQAVELVVDETIKDRTPAKELTN
                     RIFEESRKRGLLIGKGGLEGNAFRIAPPMTVNASEVDEALGILRESFEAAGAN"
     gene            complement(3107..>3866)
                     /locus_tag="VF958_01000"
     CDS             complement(3107..>3866)
                     /locus_tag="VF958_01000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020579576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX9147713.1"
                     /translation="YHALTRPARAEGEATHRAIALAEIAGVPIYIVHLSAAEALEMVT
                     EARDRGLPAYAETCPQYLFLSYDNYEEPGFEGAKYVMSPPLRPKETQDRLWRGLAGGD
                     LQAISTDHCPFCMKEQKELGKGDFSKIPNGAPGIETRMSLVFDGGVRTGKISLNRWVE
                     LTSTSPAKIFGLFPRKGTIAPGSDADVVVFDPNGKQTLSAKTLHMKVDYNPYEGREVT
                     GVTETVLSRGRVIVENGKFVGRAGAGSFLKRKPR"
BASE COUNT          588 a         1268 c         1272 g          738 t
ORIGIN      
        1 cagaggtaga cgtcgctgtc ggaagtgccg tttgtcttca cgagcgcgac cgacttgccg
       61 tcgggggaga tcgccccgat ctgatatccc tcgttctgat agaccagcgc tcgctcgtac
      121 gtcttcgcat cgtagcgata gaggtcgaag tacttcgggt cgcgctcgtt cgtctcgacc
      181 cagaaagccg tgcgatcccc cgtccagccg tcgaagtcgg ccttcagctt gtcgcccggc
      241 gtcagatccc gctccttgcc gtcggcctcg cggacgtaga ggtggttcaa ctcgttgccc
      301 ccctggtcgc gcgtgtagag cacccgatcg tcgccgggga aaaacgaaac ggcgtaggtc
      361 gtgtcggttg ccgacgacgt caggtttgtc ggcgccccgc cggtcgcggg cacggaatag
      421 acgttgtaga cgcccgtctg gttcgaggag aagaggatgc gcttctcgtc agacgagaag
      481 gacgcgtcgc ggacgccggt cgtcgcgaag aactgctcga tcgtgtactg cttcgggact
      541 cgtgcagcct tcttcggagc cgccggcgcc gcggcgagga gggtgaggga cgacgacaga
      601 acgattgcgg cggcgatggc gagtcttctc ctcatttgat gctcctaccg gcggccgccg
      661 gggcttcgtt gtcgtaccca ccgtggtagt cggagtcgtc gggcctcttg atctgcgagt
      721 gcggcacgat ccggttgcgt cgcagcccgc ggaagtcttc gaggttcttc caaccccggt
      781 gcgcgttctt ctcaaggaac tcgctcatgc cggcgaccag gttcctgatc acgttcggac
      841 cgacggcgtg gtcgagcatc gccgccgtgc acacctggac cgtcccgcag ccgagcagga
      901 agtagttgag cgcatgcgcg aactcggcga tgcctccgat ccccgagaac tccttatccg
      961 ggaacgcgcc ggtcatctgc gccatcttcg ccagcgagag cggcaggatc gcgggcccgc
     1021 cgaggccgcc ggtcgagacg tagccctcga cgttcatctc gaactcgagc gtctccgggt
     1081 cgatgaccgg cagggacgta aacgtgttgg aggatgagat cgcgtcggcg ccgccgagga
     1141 atgcgccccg cgcctcgacg acgatgtcgg tcgtcgaggg cgtgagcttc gcccacacag
     1201 gtaccttcgc cacctccttg acggcctgcg tgacgacgga gatcaactcg tcgtccttgc
     1261 cgatgttcga gcccatgtcg ggacgatcca tgtgcgggca cgagaggttc aactcgagcg
     1321 cgtcggcacc gtcctcctga caggccttcg cgagcgtctg ccagtgccgc atctcctcgt
     1381 cggagccgga tcccgccatg atcgacgcga cgagcacctt cgtcgggtgc cccttcttga
     1441 tgcgcgcgag ccggccgatc caccagtcga gcggcttgtc ggagatgagc tcccagttcc
     1501 aggacgagtg gagcgcggtc cccggcttct tcttcatcga gatgtgcccg gtctcagggt
     1561 cggtgcgcag gaagttcgtc ttcggcccct tgacgttcac gaccgggtga accccgatcg
     1621 tcttcgtgac gacccccgcc catcccgctt cgaacgcgcg caggatgttc gactccgact
     1681 cggtcggcgg cgccgacgag agcaggaacg ggttctcgaa atcgatgccg gtgaacttga
     1741 cggagaggtc gggcatggga acctccaggt tctcagttcg ccccggcggc ctcgaacgat
     1801 tcgcgcagga tcccgagcgc ttcgtccacc tcggaagcgt tcaccgtcat gggcggtgcg
     1861 atgcggaacg cgttcccttc gagaccgccc ttgccgatca ggaggccgcg cttgcgcgac
     1921 tcttcgaaga tccggttggt cagctccttc gcgggcgtgc ggtccttgat tgtctcgtcg
     1981 acgacgagct cgaccgcctg catcaggccc atgccccgca cgtcgcccag gttcttcggg
     2041 aaccggcgct tcagctcctc gaggccgccc cggagccgtc gacccatcgt ctcggcgttg
     2101 tccggcaggt tctcttcctc gatgatctgg atcgtcgcat tggccgccgc gcaggagacg
     2161 gggttgccgc cgaacgtcga gatcgtgagc gccttcaggg acgccgcgat ctccggagtg
     2221 cagatcgtca cgccgagcgg caggccgttc gccacgcctt tcgcggtcgt catgatctcg
     2281 ggctcgacgc cccagtgctc gatgccccac atcttcgtgc cggttcggcc gaagccggtc
     2341 tggacctcgt cgcagatgaa gacgccgccg tacttgcgga tgatcgcgac ggcagcttcg
     2401 aaatactccg ccggcggcgt gatgaacccg ccgacgccct ggatcggctc ggcgaggaac
     2461 gccgagatgc gcccggtcgt cgtcgtgcgg atgatgtcct cgatgtcttt cgcgcaggcg
     2521 agcccgcacg aggggtactc gaggtgcatc gggcagcggt agcagtacgg cgtcggggcg
     2581 tgcttgacgc cggcgatctt cgtcggcaac gctctccaac tggagtgcgc agtgagcgac
     2641 tgcgcgagca tcgatcggcc cgagtaaccg tggcgcagcg cgatcaactc cgaccgaccg
     2701 gtgaagagct cggccatcat caccgcggtc tcgtccgcct cggttccgga ggccgtgaaa
     2761 tacgtctggc gcaagtttcc cggcgcgagc cgcgcgagcc gctcggccag ctcgacgacc
     2821 ggcagcgtcg gatagagcgt cgagatgtgg gagaggcggt cgacctgggc tttgaccgcg
     2881 gtgttgacct tctcgttggc gtgtccgacg gagaccgtga ggatgccgcc gaagaagtcg
     2941 aggtactcgt tgccgtcggc gtctcggacg cggagcccct ttgcctcggt gaccacgatc
     3001 ggttcctggt agtactggat cgtcgcggga aacaggtact ccttgtgctt ctcgcggacg
     3061 gtccgggaaa ggtcggacac ggggcgggtc tggcggatct cggtgctcat cttggcttcc
     3121 ttttcaagaa cgagccggcg ccggctcgtc cgacgaattt tccgttctcg acgatcacgc
     3181 ggccgcggga caacaccgtt tcggtcaccc cggtgacttc tcttccctcg tacgggttgt
     3241 agtccacctt catgtggagc gtcttcgccg acagcgtctg ctttccgttc gggtcgaaca
     3301 cgacgacgtc cgcgtccgag ccgggcgcga tcgtcccctt cctcgggaag agaccgaaga
     3361 tcttcgcagg cgacgtggag gtcagctcga cccaccggtt caacgagatc ttgccggtcc
     3421 ggacgcctcc gtcgaagacg aggctcatgc gggtctcgat gcccggcgcg ccgttgggga
     3481 tcttcgagaa atctcccttc ccgagctcct tctgctcctt catacagaac gggcagtggt
     3541 ccgtcgagat cgcctggagg tcgcccccgg cgagcccgcg ccacagtcgg tcctgcgtct
     3601 ccttcggtcg cagcggcggg ctcatgacgt acttcgcgcc ttcgaaaccg ggctcttcgt
     3661 agttatcgta cgagaggaag aggtactgcg gacacgtctc ggcgtaggcc gggagtcccc
     3721 ggtctcgcgc ctccgtgacc atctcgagcg cctcggccgc cgagaggtgg acgatgtaga
     3781 tgggaactcc cgcgatttcc gcgagtgcga tcgcgcggtg cgtggcctcg ccctcggcgc
     3841 gcgcaggccg tgtcaacgcg tggtac
//
LOCUS       DASWAV010000047         2396 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U2665
            k141_762876, whole genome shotgun sequence.
ACCESSION   DASWAV010000047 DASWAV010000000
VERSION     DASWAV010000047.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960316
            Sequence Read Archive: SRR10260007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2396)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2396)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:47:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,929
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2396
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_762876"
                     /isolate="SMAG_U2665"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.36444 N 132.31889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(278..1249)
                     /locus_tag="VF958_01005"
     CDS             complement(278..1249)
                     /locus_tag="VF958_01005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002169108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent alcohol dehydrogenase"
                     /protein_id="HEX9147714.1"
                     /translation="MKAIVYTQYGPPEVLRLAEVGKPVPRDNEVLIRVHATTVSAADS
                     RCRSFTVPRSFWIPARLALGILKPRRSILGGELSGEIEETGKDVTRFEKGERIFAATL
                     MRFGAYAEYTCLPEAGVIAAMPTNMTYEEAAAVPIGARAALHFLKKANIKSGQRVLVY
                     GASGSVGTFAVQLARYYGAEVTAVCGGSNLELVKSLGADRAIDYTRVDFAAKGETYDV
                     IFVAVDKSRFLDCMKALKRKGIYLNATTPVRTLPMRWAALTSGKTIVTGEHPSEKADD
                     LVFLKGLIEEGALRSAIDRRYTLERIVEAHRYVDQGHKKGNVVITVE"
     gene            1639..>2396
                     /locus_tag="VF958_01010"
     CDS             1639..>2396
                     /locus_tag="VF958_01010"
                     /inference="COORDINATES: protein motif:HMM:NF012335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent alcohol dehydrogenase"
                     /protein_id="HEX9147715.1"
                     /translation="MRAVICTRYGPPEVLRLEELETPTPQKNELRIRIFATAVTSSDC
                     YLRGLSLTFAYRIMARIALGWTAPRRRVLGMVLSGEVDAVGPDVGSFKAGDQVFGFNR
                     HLFGTYAEYVCWPEDGLLATRPANLTNEEAASIPYGGLLALHVLRRANVQAGQRVLVY
                     GASGAVGTSAVQLAKHFGAEVTGVCSTANLALVRSLGATRVVDYTREDFTARGPRYDL
                     IFDAVGKKKSAAGLRQCDRALASGGRRLSVDDGTP"
BASE COUNT          387 a          722 c          780 g          507 t
ORIGIN      
        1 tgatgatcga cgggatcggt gtcctgatct ggttccttca ggccctgcta ctgccggccc
       61 accccgcgat ccactacccc gggctcgccg tgagcttcat cgctgaagtc gggctggcat
      121 tgtggctcct cgtcaagggt gtgaaggtcg tcgactccgg agcaggcctg ccgggctgat
      181 ccgagccgtt cgacgtagcc gccatcaggg cctgttcgtc tagcgatgcc gcagccaggc
      241 aatctggaag cgcagtcgtt gcgggagggg agatccttca ctcaaccgtt atgacgacgt
      301 ttcccttctt gtgtccctgg tcgacgtagc ggtgagcttc gacgatgcgc tcaagcgtgt
      361 agcgccggtc tatggccgaa cgcagtgctc cctcctcgat caagcctttg aggaagacga
      421 ggtcgtcggc cttttcgctc gggtgttctc cggtgacgat tgttttgccg ctggtcaggg
      481 ctgcccaccg catgggaagg gtgcgaaccg gcgtggtggc gttcaggtaa atgcccttcc
      541 gctttagcgc cttcatgcag tcgaggaagc ggctcttgtc cactgccacg aagatgacgt
      601 cgtaggtttc gcctttggcg gcgaagtcta ctcttgtgta gtcaatcgcg cgatcggcac
      661 caagggattt caccagttcg agattcgagc ccccacatac ggcggtgact tccgctccgt
      721 agtatctggc gagctgcacg gcgaacgtac cgacgctgcc cgaggccccg tagacgagga
      781 ctctttggcc gctcttgatg ttcgccttct tgagaaagtg cagggccgcg cgggctccga
      841 tcggcacggc agcggcttcc tcgtaggtca tgttggtcgg cattgcggcg atcacaccgg
      901 cttcggggag gcacgtgtac tcggcgtagg cgccgaatct catgagggtc gccgcgaaga
      961 tccgttcgcc tttctcgaat ctggttacgt ccttgcctgt ctcttcgatt tcgccggaga
     1021 gctcgccgcc gagaatcgac cgtctgggct tcagtatgcc gagggccagg cgggcgggga
     1081 tccaaaacga gcggggcacc gtgaagctcc tgcaccggga gtctgccgcc gatacggtgg
     1141 tcgcgtgcac tcgtatcaac acttcattgt cccggggaac cggttttccc acttcagcga
     1201 gacgtaggac ttctggcggg ccgtactgtg tgtagacgat cgccttcatg ggcactccct
     1261 atcttcttgc caccacggtc cggctccggc tccccatttt cctttgccct gtgttcttgt
     1321 cgttcccggt agcctcggct ctgggcgaat gacaggcaat tcgcgtcggt tccccgcgtc
     1381 gcgcttgtcg ccatgacgta gcgttccggc ggcgtgccgt caagaaaccg tctacgtgat
     1441 agtgccgtgc ttgcacattg tcggtcgctg gatttcgacg acgatcgcgg gagccccctg
     1501 actctgttca gcagctggca agcgcctgaa gtgtttgacc ttgaccagaa ccgtcttgcc
     1561 gtgctgcgct tgcagctgaa accatagccg ttcgagggag aagcgaacgc gggattgcag
     1621 gcagaaggga cttggcagat gagagcggtc atctgcacga ggtacggtcc gccggaagtc
     1681 ctccggctcg aggagttgga aaccccgact ccgcagaaga acgagttgcg cattcgcata
     1741 ttcgcgacgg ccgtgacatc gagcgactgc tacctccggg gcctgagtct caccttcgcg
     1801 tatcggatca tggcgcgaat cgcgctcggc tggactgcac cgcgacgtcg tgtgctcggc
     1861 atggtgctct cgggggaggt cgatgcggtc gggccggacg tcgggtcgtt caaagcgggc
     1921 gaccaggtgt tcggcttcaa ccggcatctc ttcggcacgt acgccgagta cgtgtgctgg
     1981 ccggaagacg ggctgcttgc gacgcgaccg gccaacctga ccaacgagga ggctgcatcc
     2041 attccgtacg gcggccttct tgcgctccac gtcttgcgac gagccaacgt tcaggccgga
     2101 cagcgggtcc tggtctacgg tgcctctggg gcggtcggca cgtctgcggt gcagcttgcc
     2161 aagcacttcg gcgcagaggt gacaggggtg tgcagcacgg cgaacctggc gctggtgcgg
     2221 tctctgggcg cgacaagggt cgtcgactac acgagagagg acttcactgc tcggggacca
     2281 cgctatgacc tcatcttcga cgcggtcggg aagaagaaga gcgcggcggg gctacggcaa
     2341 tgcgatcggg cactggcctc tggtggcagg cgtctctcgg tcgatgatgg aacacc
//
LOCUS       DASWAV010000048         4799 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U2665
            k141_602987, whole genome shotgun sequence.
ACCESSION   DASWAV010000048 DASWAV010000000
VERSION     DASWAV010000048.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960316
            Sequence Read Archive: SRR10260007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 4799)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4799)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:47:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,929
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4799
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_602987"
                     /isolate="SMAG_U2665"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.36444 N 132.31889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..318)
                     /locus_tag="VF958_01015"
     CDS             complement(<1..318)
                     /locus_tag="VF958_01015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011525312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M13 family metallopeptidase N-terminal
                     domain-containing protein"
                     /protein_id="HEX9147716.1"
                     /translation="MQTFFRSTARLISAAAVAAIVLNAPPARAAEHEIGSSTAAPGEP
                     GKARLTTLDVAGMDSGSQACTDFYQYANGGWLSSNPIPSDRPRWGTFDELFQRNQNDL
                     RTIL"
     gene            complement(428..1372)
                     /locus_tag="VF958_01020"
     CDS             complement(428..1372)
                     /locus_tag="VF958_01020"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM/SPASM domain-containing protein"
                     /protein_id="HEX9147717.1"
                     /translation="MSRGKNGLRLPTLKYMLELTSRCNLRCGMCPMDALTRPFEDAPW
                     DMVEDVSRQMKSLGLKMRYLHEMGEPFLYKRLPEAIDLFPGVCVSTNATLLSEEWAKK
                     VLDTSLSKIRLCIDTLKPDVYPIVRKGGKFDVVVENIRRFLEMSKGKKIVVEIQRMVT
                     TQTHDESVRDFREFFRLDRYPQATIIQKTCEGLDTSEVTELHQAYYGCFQGTPYQWFV
                     ILCNGDVTHCCYDYDGTQKLGNVRRNSIEEIAASPFLDTIEDGFRDHDFSKLPRCAEC
                     FKNPAAKPPLTDRIWRYAQLFPWKDPLRRFLNVPGGEN"
     gene            complement(1369..2481)
                     /locus_tag="VF958_01025"
     CDS             complement(1369..2481)
                     /locus_tag="VF958_01025"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HEX9147718.1"
                     /translation="MSVARLKQGLALARDWIAPGDAIAQWTHFVTSVCNARCAHCFYP
                     INAGKNELTLDEIERFASTLPPIRLLLISGGEPFLRRDLPEIIRIYFERCGVLNVGVP
                     TNGFAAAEIAGMVERICSFSPDLSLGVSVSIDGFRDFHDRVRAVPGLYDRALATLESL
                     LEVARSTPNLTVGVTTVFMRDNQAEVEEFCRFVHETYRPSHHSLGLIRGDAYDPALKE
                     GLDVDRYEQLSRWLDARYPPDEARTGWRGARTRARREVNRRRFEYIARQARGGGFEGF
                     CLAGEREFVLTEDGVVHGCELIGTPLGGVREAGYDFGNIRGNGGVRRFVDDKHERLCR
                     CTHECNVRTMILFDRANALPVIAAMAGLEPPRTKPS"
     gene            complement(2482..3393)
                     /locus_tag="VF958_01030"
     CDS             complement(2482..3393)
                     /locus_tag="VF958_01030"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEX9147719.1"
                     /translation="MHFSVVIPCRNAERTVGDAVRSALEQTEPPLEVIVVDDASDDGS
                     GDAARRAGARVLRNSRRRNAGGSRNSGMEAARGDVFAFLDADAVAPRDWLERARKTFQ
                     ADAAVAGVGGRIANGRPGRWGELDWFLNHSEWIRAGEPGPRKNIPTMGIAYRRDAVGD
                     VRFPETNSGEDTAFALAVLARGGKLWFDPEIVLTHRHERLDPQAFREKQAACGKTIYR
                     TRVALDRPGRVLVKWPALLFLFPHLWVTVARMARAGYAGRAVTLFPWLLSGEIHRIRG
                     FFQARRETKNAASVGDALTLPSPGGRG"
     gene            complement(3402..4118)
                     /locus_tag="VF958_01035"
     CDS             complement(3402..4118)
                     /locus_tag="VF958_01035"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX9147720.1"
                     /translation="MAFRKDLYPDEYFERLHRVTPFAAPQRKWRERDRDILSLAKPSP
                     EKTALDLGSALGDVCFLLAPLVKEAIGVDASAHAIELAERRRRERGLENVRFLRADVA
                     DLAGVADGSVDVAGAFDLLEHVDDETVRRMLRALRRVLKPNGVFVAYTPNREHYVERL
                     KAHDLILKQFPEHIAVRRPREIRRLLEEEGWRLRSIRYSPAPFPVVRWIERALWRLPL
                     IGRFFRYRILAKAAPDPAVH"
     gene            4195..4620
                     /locus_tag="VF958_01040"
     CDS             4195..4620
                     /locus_tag="VF958_01040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9147721.1"
                     /translation="MKTPWEKFSLPGDRPAYACGESPEFRRALREIFGTLVELVEKWP
                     AGPSEWLMTEAPAQGSLTLFFRELERRLAPAGSLWVVFPKKPFMEELQFPFSWTDVQK
                     AGLACGLVDNKIAAFSPRLTSIRFVIPLALRKSRQQVAR"
     gene            4617..>4799
                     /locus_tag="VF958_01045"
     CDS             4617..>4799
                     /locus_tag="VF958_01045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018288013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEX9147722.1"
                     /translation="MESSPVTNAVSIVGAGLAGSECAWQLARHGHRVRLHEMRPHVTT
                     AAHQTGRCAELVCSNSF"
BASE COUNT          787 a         1582 c         1629 g          801 t
ORIGIN      
        1 caggatcgtg cgcaggtcgt tctggttgcg ctgaaacagt tcgtcgaagg ttccccagcg
       61 cggccggtcc gacgggatag ggttcgacga cagccagccg ccgttggcgt actggtagaa
      121 gtcggtgcag gcctggcttc cgctgtccat gccggcgacg tccagggtcg tcagccgggc
      181 cttccccggt tcgccgggcg ccgcggtcga ggagccgatt tcgtgctccg ccgcgcgggc
      241 aggaggcgcg ttgaggacga tcgcagccac agcggccgcg ctgatcagac gggccgtcga
      301 acgaaaaaag gtctgcatgt ctcctccggg gcaggtttcg agcccgccga tcgggtgctt
      361 acggaaagga atcgggagag ttgcggtcga gggacgagat ctcgaatcgc cgggacggag
      421 cgggcggcta attctccccg cccggcacgt tcaggaatcg tcgcaggggg tccttccacg
      481 ggaagagttg cgcgtaccgc cagatccggt cggtcagggg cggcttcgcg gccgggttct
      541 tgaaacactc cgcgcagcgg gggagcttcg agaagtcgtg gtcgcggaag ccgtcctcga
      601 tcgtgtcgag gaacggcgag gcggcgatct cctcgatcga gttccgccgg acgttgccga
      661 gcttctgggt gccgtcgtag tcgtagcaac agtgcgtgac gtcgccgttg cagaggatga
      721 cgaaccactg gtaaggcgtg ccctggaagc agccgtagta ggcttggtgc agctcggtga
      781 cttccgaggt gtcgagaccc tcgcacgtct tctggatgat ggtggcctgc gggtaacggt
      841 ccagccggaa gaactcgcgg aagtcccgga cagactcgtc gtgggtctgc gtcgtgacca
      901 tgcgctggat ctcgacgacg attttctttc ccttcgacat ctccaggaat cgccggatgt
      961 tctcgaccac gacgtcgaac ttcccgccct tgcgaacgat cggatagacg tccggcttca
     1021 acgtgtcgat gcagagccgg attttcgaga gggacgtgtc gaggaccttc ttcgcccact
     1081 cctcggagag gagcgtcgcg ttcgtcgaaa cgcaaacgcc ggggaagaga tcgatcgctt
     1141 cgggcaaccg cttgtagagg aacggttcgc ccatctcgtg caggtatcgc atcttcaaac
     1201 cgagcgactt catctggcgg gagacgtcct cgaccatgtc ccacggcgcg tcctcgaacg
     1261 ggcgcgtcaa ggcgtccatc gggcacatcc cgcagcgcag gttgcaccgg ctcgtgagct
     1321 cgagcatgta cttcagcgtc gggagccgga gaccgttctt tccgcgactc acgacggttt
     1381 cgttctcggg ggctcgagcc ccgccatcgc cgcgatcacc gggagcgcgt tcgcccggtc
     1441 gaagaggatc atcgtccgca cgttgcactc gtgcgtgcag cggcagaggc gctcgtgctt
     1501 gtcgtcgacg aatcgccgca cgccgccgtt gccgcggata tttccgaagt cgtatccggc
     1561 ctcgcgcacg ccgccgaggg gcgtgccgat gagctcgcag ccgtggacga cgccgtcttc
     1621 cgtcaacacg aactcgcgct cgcccgcgag gcagaagccc tcgaacccgc cgccgcgcgc
     1681 ctgccgagcg atgtactcga agcggcggcg gttgacctcg cgccgtgcgc gtgttctcgc
     1741 gccgcgccag cccgtgcgcg cctcgtcggg gggatagcgc gcgtccagcc agcgcgagag
     1801 ctgctcgtag cggtcgacgt cgaggccctc cttgagcgcc gggtcgtacg cgtcgccccg
     1861 gatcaatccg agagagtggt ggctcggccg ataggtctcg tgcacgaagc ggcagaattc
     1921 ctcgacctcc gcctggttgt cccgcatgaa gactgtcgtg acccccaccg tcaggttcgg
     1981 cgtcgagcgc gctacctcga ggagcgactc gagcgtggcg agggcgcggt cgtacagccc
     2041 gggaacggct cgaacgcggt cgtgaaagtc gcggaatccg tcgatcgata cggagacgcc
     2101 gagcgaaagg tcgggggaga acgagcagat cctctcgacc atcccggcga tctccgccgc
     2161 cgcgaacccg ttcgtcggaa cgcccacgtt caagacgccg cagcgctcga aatagatgcg
     2221 gatgatctcg gggaggtcgc ggcggaggaa cggctcgccg ccggagatga gaaggaggcg
     2281 aatcggcgga agcgtcgagg cgaagcgctc gatctcgtcg agcgtgagct cgttctttcc
     2341 cgcgttgatc ggataaaagc agtgcgcgca gcgcgcgttg cagaccgacg tcacgaagtg
     2401 cgtccactgc gcgatcgcgt cgcccggcgc gatccagtcc cgcgcgagcg cgaggccctg
     2461 cttcagacga gccacagaca tttaccctct ccccccggga gagggcaggg tgagggcgtc
     2521 cccgacgctc gcggcgttct tggtctctct tcgtgcctgg aagaagcctc ggatgcgatg
     2581 aatctcgccg gagagaagcc acggaaagag cgtcaccgct cgtcctgcgt atcccgcccg
     2641 tgccatccgc gcgaccgtga cccacaggtg cggaaagagg aagaggagcg ccggccactt
     2701 cacgagcacg cgccccggcc ggtcgagcgc gacgcgagtc cggtagatcg tctttccaca
     2761 cgcggcctgc ttctcgcgga acgcctgcgg atccagccgt tcgtggcggt gcgtgagcac
     2821 gatctccggg tcgaaccaga gcttcccccc gcgcgcgagg acggcgagcg cgaaagccgt
     2881 atcctcgccg gaattcgttt cggggaagcg cacgtcgccg acggcgtccc gacggtaggc
     2941 tattcccatc gtcgggatgt tctttcgcgg acccggttct ccggcgcgga tccactcgga
     3001 gtggttcaag aaccaatcga gctcgcccca gcgtcccggc cgcccgttgg cgatccggcc
     3061 gccgacgccg gcgaccgccg cgtccgcctg gaacgtcttg cgcgcgcgct cgagccagtc
     3121 gcggggtgcg acagcgtcgg cgtcgagaaa cgcaaagacg tcgcctcgcg ccgcctccat
     3181 cccggagttg cgcgagccgc cggcgttgcg gcgtcgggag ttacggagga ctcgcgcgcc
     3241 ggcccgacgc gcggcgtccc ccgacccgtc atccgaggcg tcgtcgacga cgatcacctc
     3301 gagcggaggc tcggtctgct cgagcgccga tcggaccgcg tcgccgaccg tgcgctccgc
     3361 gttacggcag ggaatgacga ccgagaaatg catggaaacg ctcagtgtac cgccgggtcg
     3421 ggcgccgcct tggcgagaat ccggtaccgg aagaacctgc cgatgagagg caggcgccac
     3481 agcgcccgct cgatccagcg aacgaccgga aacggcgccg gagagtatcg gatcgagcgc
     3541 aggcgccacc cctcttcctc caggagccgc cggatctccc gcggccgccg caccgcgatg
     3601 tgctcgggga actgcttcag gatcaggtcg tgagccttca gcctctcgac gtaatgctcc
     3661 cggttgggcg tgtacgcgac gaagacaccg ttcggtttca gaacccgtcg cagcgcgcga
     3721 agcatccggc gcacggtctc gtcgtccaca tgctcgagaa gatcgaacgc accggcaacg
     3781 tcgacacttc cgtctgcgac gccggcgagg tccgcgacgt ccgcgcgcag gaaacggacg
     3841 ttctcgaggc cgcgctctcg tcgccgcctc tccgcgagct cgatcgcgtg cgccgacgcg
     3901 tcgactccga ttgcttcctt cacgagcgga gcgagcagga aacacacgtc tccgagcgcg
     3961 ctgccgagat cgagagcggt cttttcgggc gacggcttcg cgagcgagag gatgtcccgg
     4021 tcccgctcac gccacttgcg ctgtggagcc gcgaacggcg tgacgcggtg cagccgctcg
     4081 aagtactcgt ccgggtagag gtcttttcgg aacgccattg agggtcaggt ctccacttgc
     4141 cacattgagg gtcaggtctg cacgtaacga ttacctgtta ggttctagcc tttcatgaag
     4201 acgccctggg aaaaattctc gctgcccgga gaccgtccgg cctacgcgtg cggcgagtcg
     4261 ccggagttcc ggcgcgcgct gcgcgagatc ttcgggacgc tcgtcgaact ggtcgagaag
     4321 tggccggcgg gaccgagcga gtggctgatg acggaggcgc cggcgcaagg ctcgctgacg
     4381 cttttcttcc gcgaactgga gaggcgactc gcgccggcgg gctcgctgtg ggtcgtcttc
     4441 cccaagaaac ctttcatgga ggagctgcag ttcccgttct cgtggacgga cgtccagaag
     4501 gcggggctcg cgtgcgggct cgtcgacaac aagatcgcag cgttctcgcc gaggctgacc
     4561 tcgatccgct tcgtgattcc gctcgcgctc cggaagtcgc gccagcaggt cgctcgatag
     4621 aatcgagccc tgtgacaaac gccgtctcga tcgtgggcgc cgggctcgcc ggctccgagt
     4681 gcgcgtggca gctagcgcgc cacggtcacc gcgttcgcct ccacgagatg cggcctcacg
     4741 tgacaaccgc cgcccaccag accggccgtt gcgccgagct cgtgtgctcg aactcgttc
//
LOCUS       DASWAV010000049         7578 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U2665
            k141_242944, whole genome shotgun sequence.
ACCESSION   DASWAV010000049 DASWAV010000000
VERSION     DASWAV010000049.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960316
            Sequence Read Archive: SRR10260007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 7578)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7578)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:47:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,929
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7578
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_242944"
                     /isolate="SMAG_U2665"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.36444 N 132.31889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            238..1356
                     /gene="lpxD"
                     /locus_tag="VF958_01050"
     CDS             238..1356
                     /gene="lpxD"
                     /locus_tag="VF958_01050"
                     /EC_number="2.3.1.191"
                     /inference="COORDINATES: protein motif:HMM:TIGR01853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-(3-hydroxymyristoyl)glucosamine
                     N-acyltransferase"
                     /protein_id="HEX9147723.1"
                     /translation="MQSRKKTGRRPASRRATAPARGTFTLGELAAAVGGRVVGDSDLR
                     LSGIRPLDDAGPGDLSWIAGERRAAGARNSRAGAVLAPSAEVAGERPAVIVDSPALAL
                     ARWLELSRRPTRHRPGVERGARVHRTAQIAKSASIAPGATVGAGARVGNRTRISEGAF
                     VGEKAQIGDDCVLHVNAVVAEGCVVGSRCILHPGAVVGSDGFGYVWDGTAHRKIPQVG
                     TVRIGDDVEIGANSAIDRATLGETSIGRGTKIDNLVQVGHNVVVGEDVILCGQVGIAG
                     SARVEDRATLAGQVGVNDHVTVGKGAIATGQAGVTGSIAAGEVVSGMPAAPHRDFLRR
                     AALVARLPELARRLEALEKRLAEMEKGGPSWRSESPKS"
     gene            1329..1766
                     /gene="fabZ"
                     /locus_tag="VF958_01055"
     CDS             1329..1766
                     /gene="fabZ"
                     /locus_tag="VF958_01055"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007356343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HEX9147724.1"
                     /translation="MEIGIAEILKILPHRYPFLLVDRILEVEPGKRVLGIKNVTFNEE
                     FFQGHFPGNPVMPGVLIVEAMAQVGGVGLLASLADRDRKLIYLIGIDRCKFRKPVVPG
                     DQLVIEVEMLSIKSRLSKCRASAKVAGALVAEAELLSTFVDRP"
     gene            1763..2542
                     /gene="lpxA"
                     /locus_tag="VF958_01060"
     CDS             1763..2542
                     /gene="lpxA"
                     /locus_tag="VF958_01060"
                     /EC_number="2.3.1.129"
                     /inference="COORDINATES: protein motif:HMM:NF003657.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008780 -
                     acyl-[acyl-carrier-protein]-UDP-N-acetylglucosamine
                     O-acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP--UDP-N-acetylglucosamine
                     O-acyltransferase"
                     /protein_id="HEX9147725.1"
                     /translation="MSVDVHSTAVVAAGARLADGVRIEPFAVVGADVELGRGTTVGAH
                     AQLEGPAVFGENNRIFPHAVLGFEPQDLKYRGEPTRLVVGSRNVFREFSTVHRGTATG
                     HGETTIGDDNYVMAYAHVAHDCRVGSGTVFANCASVAGHVEVGDRAILSAFAAVHQFV
                     RVGRAAFVGAFAQCRQDILPFCKTDGVDAKTYGINTIGLKRQGLPDETIEALQKAYRL
                     LVKSKLNTTQAIERIEKEIPPDPAVGELVRFIRESKRGFHR"
     gene            2547..3503
                     /locus_tag="VF958_01065"
     CDS             2547..3503
                     /locus_tag="VF958_01065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020715828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEX9147726.1"
                     /translation="MTGVRLAVAGVGHLGRHHARVAASLAGVHVVGVYDHHPGRAEEV
                     AREFSLPILPDLEAVVREAEAVVVATPTLTHAEIAGFCLDRGLHVLVEKPITLRVSEA
                     DDLLARARRARRVLQVGHVERYNPAIEAALGLVSSPRFIEVHRLGVFTARSLDVDVVL
                     DLMIHDLQIVSCLVGRPVREVRAAGVPVLTTKLDIANARLEFEGGCVANLTASRVSAD
                     KVRKCRVFAPSIYVSVDMQAQSVKAYRLASDSGRPEIHPVAVSVSREEPLARELADFR
                     RAIVDGARPLVSGEDGRDALVLAERVLDAVEEHRATLEEAPV"
     gene            3500..4369
                     /gene="mtnP"
                     /locus_tag="VF958_01070"
     CDS             3500..4369
                     /gene="mtnP"
                     /locus_tag="VF958_01070"
                     /EC_number="2.4.2.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010941773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017061 - S-methyl-5-thioadenosine
                     phosphorylase activity [Evidence IEA];
                     GO_process: GO:0006168 - adenine salvage [Evidence IEA];
                     GO_process: GO:0019509 - L-methionine salvage from
                     methylthioadenosine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-methyl-5'-thioadenosine phosphorylase"
                     /protein_id="HEX9147727.1"
                     /translation="MRGRIGLVGGSGISDFSAIEALTEETLETPFGAPSGPYFAGTLG
                     GVPVAFLSRHGEGHRVSPTEINYRANVWGFKALGCDALLSASAVGSLKEDLPPRHAVI
                     PDQFIDRTRHRPDSFFGHGLVAHVSFAEPVCPALSRALEEAAAAAGLPARRGGTYVCM
                     EGPQFSTRAESRLYRSWGADVIGMTNLTEAKLAREAEICYATLALVTDWDAWRESEEA
                     VSVETVLAILGENAAAARRTMREAVSRVAPDRDCACRRALQFGILTDPAAIAPETRRR
                     LEPLVGKYLDRDR"
     gene            4380..5405
                     /locus_tag="VF958_01075"
     CDS             4380..5405
                     /locus_tag="VF958_01075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase family protein"
                     /protein_id="HEX9147728.1"
                     /translation="MNSSPLPLPDSGRIVVTGSVAFDYLMRFPGRFVEHLVPDRMDRL
                     SVSFLVDEMRRVPGGCASNIAYGLALLGERPLLIATAGRDAGDYRDWLAREGVDVSGL
                     VLHPDLFTASFFVSTDRDQNQLASFYTGAMARARDLTLRDAAPPDVALVVISPNDPDA
                     MSRYAAECRELGIPFVYDPSQQVARLSGEDLLAGADGALLLISNDYEFGILQQKTELS
                     REELEERLPVLVTTHGANGSTISVAGPRGRERHDVPAARLVRPAVDPTGVGDAYRAGL
                     VRGIRIGAPWEVAGRIGSIAAAFVLETLGPQAERYEPDDFVSRYVESFGDEPTLERLF
                     STDDTTG"
     gene            5434..5934
                     /gene="ribH"
                     /locus_tag="VF958_01080"
     CDS             5434..5934
                     /gene="ribH"
                     /locus_tag="VF958_01080"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010942334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009349 - riboflavin synthase complex
                     [Evidence IEA];
                     GO_function: GO:0000906 - 6,7-dimethyl-8-ribityllumazine
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HEX9147729.1"
                     /translation="MSNRAVRRVVPSFAPRERSGSLDARGSKLGLVCARWNPTVTDAM
                     LAGALGAIERHGGRPGDVLLVRVPGAFELPAAARALIASGRPDAVIVLGAIVRGETTH
                     HSVLANAVATALANLSAETGVPVGFGLLTCDTMEQARSRVSKGAEAAEAAIEMASLRR
                     TLARKS"
     gene            5936..6358
                     /gene="nusB"
                     /locus_tag="VF958_01085"
     CDS             5936..6358
                     /gene="nusB"
                     /locus_tag="VF958_01085"
                     /inference="COORDINATES: protein motif:HMM:TIGR01951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HEX9147730.1"
                     /translation="MGARRKARELALQMLFEHDVAGTAPDDMFRRSGDLHKVSEGVRD
                     FTKRLVSGTLEHRDSLDAMISKQAEHWRLMRMPIVDRNILRMALFELLHEPDTPRPVV
                     IDEALEIAKRFSTPRSSQFINGILDGVLKSGRSDLAKT"
     gene            6355..7140
                     /locus_tag="VF958_01090"
     CDS             6355..7140
                     /locus_tag="VF958_01090"
                     /inference="COORDINATES: protein motif:HMM:NF013961.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase-like domain-containing protein"
                     /protein_id="HEX9147731.1"
                     /translation="MSRRLAVWLAAGAIAAGSAAADEVASYRGQGDAARLFEQYDNDG
                     YSLAVLPGKPGTVELSVRVSDSPLESRAPFPTGLPRDRALPPSPERDAFALRLASGAT
                     TQADAVERILSGLAAFVRYDPDRVRRQEPSAVFAERHANCVGLSELAVDLLRRVGISA
                     RTVQGVLKTEPGSAAYDAAIGGAYHRWIEVHYPDRGYVFSDPSASVNGVDARYLPFSR
                     RAYVRPRELRVIPLALTGKLEYRSISAGSAHLRARATAIVPAP"
     gene            7137..>7578
                     /locus_tag="VF958_01095"
     CDS             7137..>7578
                     /locus_tag="VF958_01095"
                     /inference="COORDINATES: protein motif:HMM:NF012361.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HEX9147732.1"
                     /translation="MTERYDPAEIEPKWQRRWAEQRVAEVDTGVPGNEFYMLNMYPYP
                     SASRLHVGHGRNYILGDALYRRERMAGRKALNPMGWDAFGLPAENAAIQGGVHPREYT
                     LANIGRMKEQLFSLGCLYDWSKELASCDPAYYRWNQWLFPRMWER"
BASE COUNT         1126 a         2583 c         2649 g         1220 t
ORIGIN      
        1 ttcaacaagt ttcagtcggg tctggtgttc gctgatgacg cggccgacat caccgaagac
       61 gtggtgaagc gcttcaacac gacggtcgcc gtccctccgg cggcggcagc gaagcccgcg
      121 gccgcggcgc ctgcggctgc gccaacgaaa aagccgtctt aagactgaaa tcggaaatcg
      181 gtagtcgaag gagcccccga tgcggggctt ctttgcttta gtactatgcc tccccgcgtg
      241 caatcccgaa agaagacagg gcgtcgcccg gcttcccggc gcgcaaccgc gccggcccga
      301 ggcaccttta ccctcggcga gctcgccgcc gccgtcggcg gccgcgtcgt tggcgattcc
      361 gacctccgcc tctcggggat ccggccgctc gacgacgcag gcccggggga cctgtcgtgg
      421 atcgcggggg agcgtcgcgc cgcaggcgcg cgaaacagcc gcgcgggcgc cgtcctcgcc
      481 ccctcggcgg aggtcgcggg ggagaggccg gcggtgatcg tcgattcgcc cgcgctcgcc
      541 ctggcgcgat ggctcgagct ctcccggcga ccaacgcgcc accgccccgg cgtcgaacgc
      601 ggggcgcgcg tgcaccggac ggcgcagatc gcgaagagcg cctcgatcgc tccgggcgcg
      661 acggtcggcg cgggcgcccg cgttggcaac cgcacccgca tttcggaggg agcgttcgtg
      721 ggcgagaagg cacagatcgg cgatgattgc gtccttcacg tgaacgcggt cgttgcagaa
      781 ggatgcgtcg tcgggtctcg gtgcatcctc catcccggtg ccgtcgtcgg gtccgacggg
      841 ttcggctacg tctgggacgg aaccgcgcac cgcaagatcc cgcaggtggg gaccgttcgg
      901 atcggggacg acgtcgagat cggcgccaac tcggcgatcg accgcgcgac gctcggcgag
      961 acgtctatcg gacgagggac gaagatcgac aacctcgttc aggtcgggca caacgtcgtc
     1021 gtcggcgaag acgtgatcct ctgcgggcaa gtcggcatcg caggttcggc ccgggtcgag
     1081 gaccgcgcca cgctggccgg gcaggtcggc gtcaacgacc acgtcaccgt cggaaagggc
     1141 gcgatcgcga ccggacaggc cggggtgacc ggatcgatcg cggccggcga ggtcgtgtcc
     1201 gggatgccgg cagcgccgca ccgcgatttc ctccggcgcg cggccctggt cgcacggctg
     1261 ccggagctcg cgcggcggct cgaagctctc gagaagcggc tcgccgaaat ggagaaagga
     1321 ggcccgtcgt ggagatcgga atcgccgaaa tcctgaagat cctcccccac cggtacccct
     1381 ttcttctcgt ggaccggatc ctggaagtcg agcccggcaa acgcgtcctc gggatcaaga
     1441 acgtcacgtt caacgaggag ttcttccagg ggcacttccc cggaaacccg gtgatgccgg
     1501 gcgttctcat cgtcgaggcg atggcgcagg tcggtggagt gggtctgctc gcgagcctcg
     1561 cagaccgcga ccgcaagctg atctacctga tcgggatcga ccgctgcaag ttccgcaagc
     1621 cggtcgttcc gggcgatcag ctcgtcatcg aggtggaaat gctttcgatc aagagccggc
     1681 tctcgaagtg ccgggccagc gcgaaggtgg ccggcgcgct cgtggcggag gcggagctgc
     1741 tgtcgacctt cgtggatcgg ccatgagcgt cgacgtccac tcgacggcgg tcgtcgccgc
     1801 gggcgcgcgc ctcgccgacg gcgtgcgcat cgagcccttt gccgtggtgg gggccgacgt
     1861 cgagctggga cgggggacga cggtcggggc gcacgcgcag ctcgaggggc cagccgtgtt
     1921 cggcgagaac aaccgcattt ttccgcatgc ggtcctcggc ttcgagcccc aggacctgaa
     1981 gtaccgcggc gagccgaccc gtctggtcgt cgggagccgg aacgtgttcc gcgagttctc
     2041 gacggtgcac cgcggcaccg cgacgggaca cggcgagacg acgatcggcg acgacaacta
     2101 cgtcatggcg tacgcgcacg tcgcccatga ctgccgggtc ggatcgggga cggtcttcgc
     2161 gaactgcgca tcggtcgcgg ggcacgtgga ggtcggggac cgggcgattc tgagtgcgtt
     2221 cgcggcggtg catcagttcg ttcgcgtcgg gcgcgccgcg ttcgtcggtg cattcgcgca
     2281 gtgccggcag gacattcttc cgttctgcaa gaccgatggt gtcgacgcga agacctacgg
     2341 catcaacacc atcggtctga agcggcaggg cttaccggac gagacgatcg aggcgctcca
     2401 gaaggcctac cggctgctcg tgaaatcgaa actgaacacg acacaggcca tcgaaaggat
     2461 cgagaaggag atcccgccgg atccggccgt cggagagctc gtccgtttca tccgcgagag
     2521 caagcggggt ttccaccggt gacggcgtga cgggagtccg cctcgccgtc gccggcgtgg
     2581 gccatctggg tcgccaccat gcgcgtgtcg ccgcctccct cgcgggcgtc cacgtggtcg
     2641 gcgtgtacga ccaccacccg ggccgcgccg aagaggtcgc gcgcgagttc tcgcttccga
     2701 tcctcccgga cctcgaagcg gtcgtccgcg aggcggaggc ggtcgttgtc gcgacgccga
     2761 cactgactca cgcggagatc gcgggcttct gcctcgaccg ggggcttcac gtcctcgtcg
     2821 agaagccgat caccctgcgc gtctcggagg ccgacgacct gctcgcgagg gcgcggcgcg
     2881 cgcgccgggt tcttcaagtc gggcacgtcg agcgatacaa cccggcgatc gaagccgccc
     2941 tcgggctcgt gagctctccg cgattcatcg aggttcaccg gctcggcgtc ttcaccgcgc
     3001 gcagcctcga cgtcgacgtg gtgctggacc tgatgatcca cgatctgcag atcgtttcgt
     3061 gtctggtcgg gcgccccgtc cgggaggtcc gggcggcggg agtgccggtg ctgacgacga
     3121 agctcgacat cgcgaacgcg cggctcgagt tcgagggcgg ctgcgtcgcg aacctgacgg
     3181 cctcccgggt ctccgccgac aaggtgcgca agtgccgtgt gttcgcgccg tcgatttacg
     3241 tgtcggtcga catgcaggcg cagagcgtca aggcgtatcg gctggcctca gactcggggc
     3301 ggccggagat ccatcccgtc gcggtctccg tctctcgcga ggagccgctc gcccgggaac
     3361 tcgccgactt ccgccgcgcg atcgtggacg gcgcaaggcc cctcgtctcc ggagaggacg
     3421 gacgcgatgc cctcgttctc gccgagcgcg tgctcgacgc ggtcgaggag catcgggcca
     3481 cgctggaaga ggcgccggtc tgaggggtcg catcgggctc gtcggtggaa gcgggatctc
     3541 cgacttctcc gccatcgagg cgctgaccga ggagaccctc gaaacgccct tcggagcgcc
     3601 gtcgggcccg tatttcgccg gaaccctcgg cggggtgccg gtcgcgtttc tttcgcggca
     3661 cggagaaggc caccgcgtct cgccgaccga gatcaactat cgcgccaacg tgtggggctt
     3721 caaggctctc ggctgcgacg cgctcctctc ggcgtccgcg gtcgggagtc tgaaggagga
     3781 ccttcctccg cgccacgctg tcatccccga ccagttcatc gaccgcacgc gacaccgccc
     3841 cgatagtttt ttcggccacg ggctcgtcgc gcacgtctcc ttcgccgagc ccgtgtgtcc
     3901 ggctctctcg cgcgctctcg aggaggcggc ggccgcggcg ggtcttccgg cgcggcgggg
     3961 cggcacctac gtctgcatgg agggtccgca gttctccacg cgcgcggagt cgcgcctgta
     4021 ccgttcctgg ggcgccgacg tcattggcat gacgaatctg acggaggcga agctcgctcg
     4081 cgaggcggag atctgctacg ccacgctcgc gctcgtgacc gactgggacg cgtggcgcga
     4141 atcggaggag gccgtctcgg tcgagaccgt gctggcgatc ctcggcgaaa acgcggcggc
     4201 ggcccgacgg accatgcgcg aggcggtgtc gcgcgtcgcg ccggaccgcg actgcgcctg
     4261 ccggcgcgcg ctccaattcg ggatcctgac cgaccctgcg gcgattgctc ccgagactcg
     4321 ccgtcgtctc gagccgctcg tcgggaagta cctggaccgt gatcggtgag gcgtcgggca
     4381 tgaactcctc cccgctcccg cttcccgatt ccggtcgcat cgtcgtcacg ggctcggtcg
     4441 cattcgacta cctgatgcgg tttccggggc gctttgtcga gcacctcgtt cccgaccgaa
     4501 tggaccggct ttcggtgtct ttcctcgtcg acgagatgcg gcgagtgccc ggcggatgcg
     4561 cgtcgaacat tgcgtacggc ctcgcgcttc tgggcgagcg gccgctcctc atcgcgaccg
     4621 cgggccgtga cgcgggagac taccgggact ggctcgcccg cgaaggggtc gacgtctccg
     4681 gcctcgtcct gcatcccgac ctcttcacag catccttctt cgtctcgacc gatcgggatc
     4741 agaaccagct cgcttccttc tacaccggcg cgatggcgag agcgcgcgat ctcaccctcc
     4801 gcgacgcggc gccgccggac gtcgccctgg tcgtgatctc tccgaacgat cccgacgcca
     4861 tgtcgcgcta cgcggccgag tgccgcgagc tcggcattcc cttcgtgtac gacccgagcc
     4921 agcaggtcgc acggctctcc ggcgaagatc ttctcgcggg tgcggacgga gcgttgcttc
     4981 tcatcagcaa cgactacgag ttcggtatcc tgcagcagaa gacggagctc tcgcgcgagg
     5041 agctcgagga gcgccttccg gttctcgtca cgacgcacgg tgcgaacggt tcgaccatat
     5101 cggtcgcggg accgcgcggt cgggaacgcc acgacgtgcc ggcggcgaga ctcgttcggc
     5161 ccgcggtcga cccgacggga gtcggcgacg cgtatcgcgc cgggctcgtg cggggcatcc
     5221 gaatcggcgc tccctgggag gtggccggca ggatcggcag catcgccgct gcgttcgtcc
     5281 tcgagaccct gggaccccag gcggagcgtt acgagcccga tgacttcgtc tcgcgatacg
     5341 tcgaaagctt cggagacgag ccgactctcg agcggctctt ttccaccgac gacacgacgg
     5401 ggtaaaatcg cttctctcga agaggattgg tccttgagca atcgagccgt gcgtcgcgtt
     5461 gtcccctcct tcgctccccg agaacggtcg gggagcctcg acgcacgcgg ctcgaagctc
     5521 ggtctggtct gcgcccgctg gaacccgacc gtgaccgacg ccatgctcgc gggcgctctc
     5581 ggagcgatcg agcgtcacgg tggccggccg ggcgacgtcc ttctcgtgcg ggtacccggc
     5641 gcgttcgagt tgcctgccgc ggcgcgcgcg ctgatcgcga gcggtcggcc ggatgcggtc
     5701 atcgtgctcg gggcgatcgt ccgcggcgag acgacgcacc actcggtgct cgccaacgcc
     5761 gtcgcgacgg ccctcgcgaa cctgtctgcg gaaacgggcg tgcccgtcgg attcggtctg
     5821 ctgacgtgcg acacgatgga gcaggcgagg agccgcgtgt cgaagggcgc cgaggcggcc
     5881 gaggcggcga tcgagatggc gagcctgcgc cggaccctcg cgcgaaagag ttgacgtggg
     5941 cgcccgtcgc aaagcgcgcg agctcgcgct ccagatgctt ttcgagcacg acgtcgccgg
     6001 caccgcaccc gacgacatgt ttcgtcgctc gggcgacctc cacaaggttt ccgagggcgt
     6061 ccgcgacttt accaagcggc tcgtctccgg caccctcgag caccgcgact ctctcgacgc
     6121 gatgatctcg aagcaggccg aacactggcg cctcatgcga atgccgatcg tggaccgcaa
     6181 catcttgcgc atggcactct tcgagttgct gcacgagccg gacacgccgc gccccgtcgt
     6241 gatcgacgag gcgctcgaga tcgcaaagcg cttctcgacg cctcgctcgt cgcagttcat
     6301 caatgggatt ctggacgggg tgctgaagtc cggtcgctcg gatctcgcga aaacgtgagc
     6361 cggcgcctgg ccgtctggct ggccgcgggc gcgatcgcgg ccggctcggc cgcggccgat
     6421 gaagtcgcga gctaccgcgg gcagggggat gccgcccggc tcttcgagca gtacgacaac
     6481 gacggatact cgctcgcagt tctcccgggg aagccgggga ccgtcgagct ctcggtgcgc
     6541 gtgagcgatt cgccgctcga gagccgagcg ccttttccga cgggcctccc gcgcgaccgc
     6601 gcgctgccgc cgtcgcccga gcgggatgca ttcgcgttgc gcctggcgtc gggagcgacc
     6661 acccaggcgg acgccgtcga acggattctt tcggggctcg ccgcgttcgt gcggtacgac
     6721 ccggaccgcg tgcggcgcca ggagccatcg gcggtcttcg ccgagcggca cgcaaactgc
     6781 gtgggtctct ccgagctcgc ggtcgacctg ctgcgtcgag tcggcatttc ggcgcgcacc
     6841 gtccaggggg tgttgaaaac cgagccgggc tccgccgcct atgacgcagc gatcggcggc
     6901 gcctaccacc gatggatcga ggtccactat cccgaccgcg gctacgtttt ctcggatccg
     6961 agcgcttcgg tcaacggcgt ggacgcacgg tacctcccgt tttcgcggcg ggcctacgtc
     7021 cggccccggg aactgcgagt gatcccgctg gcgctgacgg gaaagctcga ataccggagc
     7081 atctccgcgg gttccgccca tcttcgggcg agggcgactg ctatagtccc cgccccgtga
     7141 ccgaacgtta cgacccggcc gagatcgagc cgaagtggca gcggcgctgg gcggagcagc
     7201 gcgtcgccga ggtcgatacc ggtgtgccgg gaaacgagtt ctacatgctg aacatgtacc
     7261 cgtacccgtc ggcgagccgc ctccacgtgg gacacggtcg caactacatc ctcggagacg
     7321 cgctctaccg ccgggaacgg atggccggcc ggaaggccct gaacccgatg ggctgggacg
     7381 ccttcggcct gccggccgag aacgccgcga tccagggagg cgtccacccg cgggaataca
     7441 cgctcgcgaa catcggccgc atgaaggagc agctcttcag cctgggctgc ctgtacgact
     7501 ggtcgaagga gctcgcctcc tgcgatccgg cgtattaccg gtggaaccag tggctgttcc
     7561 cccggatgtg ggagcgcg
//
LOCUS       DASWAV010000050        13753 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U2665
            k141_602995, whole genome shotgun sequence.
ACCESSION   DASWAV010000050 DASWAV010000000
VERSION     DASWAV010000050.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960316
            Sequence Read Archive: SRR10260007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 13753)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13753)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:47:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,929
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13753
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_602995"
                     /isolate="SMAG_U2665"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.36444 N 132.31889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            96..341
                     /locus_tag="VF958_01100"
     CDS             96..341
                     /locus_tag="VF958_01100"
                     /inference="COORDINATES: protein motif:HMM:NF014608.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HEX9147733.1"
                     /translation="MRKVSAAEGRLIGEAVGEIEKEEGVLVIKRIHLRLKLKAAESER
                     ETAERVHGFYQDRCPVYRSLRAAIAITTELVFEPLDG"
     gene            420..1268
                     /gene="fdhD"
                     /locus_tag="VF958_01105"
     CDS             420..1268
                     /gene="fdhD"
                     /locus_tag="VF958_01105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013138783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory
                     sulfurtransferase FdhD"
                     /protein_id="HEX9147734.1"
                     /translation="MGRVEPIERVRVVAREGQRQVERDDALAVEEPLEIRIRPAGPDR
                     PKSFVTTMRTPGNDEELVAGLLFAEGVVVSRADLLRLERPLDPRIDPELRANVMIATL
                     APDAQARGEKLERGTVMGSACGVCGKTSIENVLPSDARPLVSNFRVDGELLFALPERL
                     REGQSVFSKTGGLHAAALFGADGRLRALREDIGRHNATDKLVGHFLLEDRLPLAESVL
                     MVSGRAGFEIVQKAYAAGIPILASVSAPSSLAVSLGEAAGLTLVGFLRERRFNVYSCP
                     ERILPT"
     gene            1381..4464
                     /gene="fdnG"
                     /locus_tag="VF958_01110"
     CDS             1381..4464
                     /gene="fdnG"
                     /locus_tag="VF958_01110"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014238293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_except=(pos:1954..1956,aa:Sec)
                     /transl_table=11
                     /product="formate dehydrogenase-N subunit alpha"
                     /protein_id="HEX9147735.1"
                     /translation="MDLTRRGLFKATAVGSAIALGFDVGRAKAEMREFKISRTTETRS
                     ICPYCAVSCGVIIHTLGDRSKNAKASVVHVEGDPDHPISRGSLCPKGITLKDDITHKN
                     RLTKPKVRRPGSDKWEDISWDEAIAKIARHVKATRDKCFVAKNAKGEVVNRTPGIAWI
                     GGCTDTNEFNFLQWKVITALGVPYRDTQARVUHGPTVASLAATFGRGAMTNGWVDIKN
                     ADVILAMGGNPAENHPVGFKWFMEAKKTRGAKLVAVDPRFTRTAATADLHAPLRAGTD
                     TAFLLGIIRYAIEKKRFHEDYVKIHTNAPYIVGEKFAFDDGLFSGFDEAKKEYDKTQW
                     GYEPDQKTTGYFLDPTLQHPRCVFQLLKKHVERYTPEMVERICGTPKESYLKVCEIVT
                     STYPPEKVGTITYALGWTQHSTAVQMIRAGAMLQLLLGNVGRPGGGMNAFRGHSNIQG
                     ATDTAGTFDILPGYLKTPPASAQSLKDYLDGSTPSVLGKIAWASMNYWSNYPKFMVSL
                     LKTQYGKAATAENDFGYSWLPKVDGNYSWMYICDDMYRGNSTRAGGQEPGPEGFISFG
                     MNPVGLGPNSKKWIAALSKLKWFVVVENVETEAAGFWKAPKEYDPPDPSKIQTEVFLL
                     PAAGFAEKDGTFTNSARWLQWKWKALDPPGQAKSDQEIVSRIFLAVRDLYKKEGGAFP
                     EAISNVTWSYSNPVEPDLGEVLKEINGKALQDIPDPNDKTKMLRTAGQQVDGFGQLQA
                     DGSTTCGNWLHSGVYTESGNNAQRRSTADPLGLGMYHNWAFSWPANRRIMYNRASADA
                     EGKPWDPKRAGITWNGEKWVGDVPDMKPDAPPGTYGAFIMLPEGVARLFSPGLNDGPF
                     PEHYEAIEAPIANPLHEKVSSNPVSKKFSSDKDVYGKSEDFPIVCTTYRLTEHFHYWT
                     QHQHSGVLNEMQPGFFVEIPEGLAKEKGIANGSQVKVTSARGSIQGVAMVTKRLIPLK
                     VNGKDMWQIGFPIHWGYAGDPDHTGPLANTLTPSAMDPNTWTPEYKSFLVRIEKA"
     gene            4477..5376
                     /gene="fdxH"
                     /locus_tag="VF958_01115"
     CDS             4477..5376
                     /gene="fdxH"
                     /locus_tag="VF958_01115"
                     /EC_number="1.17.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009088325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008863 - formate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0045333 - cellular respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase subunit beta"
                     /protein_id="HEX9147736.1"
                     /translation="MATQNLEIRRSSASVWGSLSGMRSVPTVSKYIDTTTCIGCKACE
                     AACIEWNDLRRVATEQTGTYQTLPTLHAEYWNLIRFNERDFDGGLIWLMRKDQCMHCE
                     EPGCLAACPAPGAIVQYANGIVDVNPDQCIGCKYCETGCPFDVPRFHQTTGKMAKCTL
                     CVDRVSVGLEPACIKACPTGCLQFGTKDDMVALGLRRVEQLKGNGFAQASLYNPDGVR
                     GTGVVTVLAHGDHPEWYRLPADPHVPWGVRFWKSILRPLGVAAMFGAVIAAFAHYTNF
                     GPKKVRGSADTDANIPSGEEVKR"
     gene            5373..6089
                     /locus_tag="VF958_01120"
     CDS             5373..6089
                     /locus_tag="VF958_01120"
                     /EC_number="1.17.1.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0008863 - formate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase subunit gamma"
                     /protein_id="HEX9147737.1"
                     /translation="MSTAAPDRAMDAAVIAGRTREDVVVGNEIVRHKRASRWIHWTVA
                     VTFILSLLTGLPIWTPIFGWMASLFGGLSVCRVVHPWVGIAFFVISGVMFFYWLADMH
                     LLPSERDWLRPARAIQYMRFQTDIEDVGKYNGGQKIFFFAVSLGAFGLLLSGLLIWFP
                     LLFPRIISELAFVIHDFTFVLFTVGIIGHIYLGTAAEPGTFGSMVRGTVTRQWARLHH
                     PSWYREITGDEARRPSSPSR"
     gene            6222..7034
                     /locus_tag="VF958_01125"
     CDS             6222..7034
                     /locus_tag="VF958_01125"
                     /inference="COORDINATES: protein motif:HMM:NF016131.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory protein FdhE"
                     /protein_id="HEX9147738.1"
                     /translation="MPTAARGSKLVSEFGRRAARAELLAREAPAAEDPLRFAAGLYRV
                     QSRVVAGIEAADAQEPLSGKLDDDADRLLDAASSVIRYAAEEGPDLLSEQARARQTDL
                     PSTARTRLLVYWAADSSSAEDYLSRAILRPYVEFLRSINRPPDRVHARGRCPFCGGAP
                     WISARRDASVMEGARRMLGCALCGGEWSFGRILCPACFEGDPAKLPSFQSEKHPAVRI
                     EACETCNRYVKSLDLSEDARPIPEVDDLVSLSMDLWAMESGFTRIEPGLAGL"
     gene            7036..7473
                     /locus_tag="VF958_01130"
     CDS             7036..7473
                     /locus_tag="VF958_01130"
                     /inference="COORDINATES: protein motif:HMM:NF013936.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043169 - cation binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HEX9147739.1"
                     /translation="MTSPDGLIAAQSGLRSRFEDFRHALDRRDEAALRLALMDFHAHL
                     LLWTTAEERVLLPALERTTFPGRDPQRELKLEYVQIRELTRYLLSQIEGHAPIADVLG
                     LVENLDRRLSAHESEMEKVYYPSAAPILTSEEWRLLTEASPPP"
     gene            complement(7479..7793)
                     /locus_tag="VF958_01135"
     CDS             complement(7479..7793)
                     /locus_tag="VF958_01135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9147740.1"
                     /translation="MSEGASGGGVALPPVPGLTNSRTVLLRRAPIRTRESGVTLSGWR
                     LSVESDEGEGAIVLVEAATGEPWYRGEGVFLGWSPEALKTAYEALRPRPDEPAFEIQQ
                     LG"
     gene            complement(7798..8820)
                     /gene="moaA"
                     /locus_tag="VF958_01140"
     CDS             complement(7798..8820)
                     /gene="moaA"
                     /locus_tag="VF958_01140"
                     /EC_number="4.1.99.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003912902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP 3',8-cyclase MoaA"
                     /protein_id="HEX9147741.1"
                     /translation="MALLDTFRRPLRNLRVSVTDRCNLRCQYCMPEKDYVWLPRSDIL
                     DFEEVSRLVDVFIGLGVDRVRLTGGEPLVRQNLQELVRLLASKSGIRDLALTTNGVLL
                     EEHAAALRAAGLHRVNVSLDTLRPERFRELARSDELERVLSGIAAASEVGFPAVKVDA
                     VVIRGVNEDELVPLLEYGRRIGAEVRFIEYMDVGGATRWSMDRVVTRAEMLGSLARHY
                     GPIVPLREETSAPADRFRLADGTVFGVISSTTAPFCGSCDRSRLTADGMWYLCLYAAR
                     GTDLRGALRAGASESELRALLEDAWHARTARGAEERLLLRKRAPLLTASALRQNPHLE
                     MHTRGG"
     gene            complement(8844..10196)
                     /locus_tag="VF958_01145"
     CDS             complement(8844..10196)
                     /locus_tag="VF958_01145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011509728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEX9147742.1"
                     /translation="MRSTNARRLSRRELVKSAGAALTLGALPALACGRRVKQPSAGRK
                     TLRILQWSHFIPAYDRWFDGVYTRAWGEKNGITVTVDHMASTEVPARGASEAAAGKGH
                     DLFLFLSPPAAYESQVIDHREVVEAVEKKHGKMISLAERSTFNPKTGKYFAFSDSYVP
                     DPGNYRTDLWRSVGFPKGPDSWEDLRVGGARIKKEFGNSVGIGLSQEIDSNMALRAVL
                     WSFGGAEQDEEGRVAINSRGTIEALKFVRALYEETMTPEVFTWDPSSNNRMILAGRAS
                     FVCNAISVTRTAERENPEMSKKIGLTSALAGPVRRIASEHVMNCYVIWKFAENPDLAK
                     QFLVDLVDHFSDVFREGESYNFPSFPGSLPDLQQRLSSDPKADPPDKYRVLGSVLDWA
                     TNVGFPGYATAAIDEVFNTFVIPTMFARVARKEVTPEAAAGDAERQIDRIFDRWKRAG
                     "
     gene            10324..10779
                     /locus_tag="VF958_01150"
     CDS             10324..10779
                     /locus_tag="VF958_01150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9147743.1"
                     /translation="MFPQIELETESGARFVPPAAETLYVFFKTTCPTSELSWPHLERI
                     RRIASTALPIFAVSQDERPETAAFNDRLGVHVETLYDPSPWKASAELGLTNVPTFFLV
                     APGGRIRETVVGFQRPKLEALAKRAGKLSGRRSVRLFPIGQNVPLIVPG"
     gene            10820..12364
                     /locus_tag="VF958_01155"
     CDS             10820..12364
                     /locus_tag="VF958_01155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014560571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEX9147744.1"
                     /translation="MPRPGNLGELKASGYRPRGVKEELRGNLRRALREGKNPFEGIYG
                     YERTVLPALHNAVLSKHDFLLLGLRGQAKTRLLRLLVGLLDPEIPAIAGSDLNEDPFR
                     PITKYGRRILSERGDDMPIEWISREERYREKLATPDVTIADLIGDVDPIKAATERRSY
                     SDEEVIHFGIIPRTNRGIFAINELPDLQPRIQVGLLNILEERDLQIRGFPIRIPLDIL
                     LVFSANPEDYTNRGNIITPLKDRISSQILTHYPDDVKTAARITAQEAWLDRDPAPAVE
                     IPEFFRELVEEITFAARTSEFVDQSSGVSARMPISAVEILASNLERRGLVHGERQVFP
                     RLCDLPACLPAITGKVEMVYEGEQQGAEVIAKKLIATAVKKLFDSRFPPPDSGRPTQR
                     RRADEDDTDELSMPRRTRSTPPPEKIHPLYEPILEWFASGNKITLSDDTPFSEHLETL
                     SSVPGLKKAVEEHFAPASKEELAVGMELVLEGLTQHLKIAREDLDSRVSYGEMLKFNL
                     VRSRTT"
     gene            complement(12371..>13753)
                     /locus_tag="VF958_01160"
     CDS             complement(12371..>13753)
                     /locus_tag="VF958_01160"
                     /inference="COORDINATES: protein motif:HMM:NF012546.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HEX9147745.1"
                     /translation="AWIYCMLSNDGRRAIAVGEKAGGGWVGTMDLRDPEHPDLTAPLV
                     RLLGNQEAGYQPMGSSGNLLYVRTDLDAPRYRVIALDMEEGAKATPRTVVPESSDTIE
                     RAVVAGDRLVLQYLADVQSRLRLFTLDGKPAGEVALPGVGAIGWFEGGSTPSFFYSFR
                     SFLLPTTVYHYDPGTGVSTPFRPPRVPFDANADETRQVFVTSKDGTRVPLFLTSKKGL
                     RRDGSNPVFLTGYGGYGASLGPAYEPDIPLWLEMGGIYALANLRGGGEYGEDWHRAGM
                     LERKQNSFDDFIAAAEYLVAERYTTPAKLAIYGHSNGGLLIGAVSTERPDLFGVAIGN
                     AGHYDMLRYHRFTVGSAWIPEYGSSDEPAAFRWLFAYSPLQNVRKGACYPATLLLAAD
                     HDDRVVPSHSYKFTAALQAAQGCDRPILVRVSSDASHGYASRREQIAERSDMWAFIAA
                     RLGVPTSRGR"
BASE COUNT         2441 a         4660 c         4358 g         2294 t
ORIGIN      
        1 ccccggacgc gttcgagcct cgcgcgacga ctctcgacta cgtcgtcgcc gcggcggcag
       61 gctgactgac cggcactttt gcaggcgcac tggaggtgcg caaggtgtcg gcggcggaag
      121 gtcggctgat cggggaagcg gtgggcgaga tcgagaagga ggagggagtc ctcgtgatca
      181 agcggatcca cctccggttg aagctgaagg cggccgagtc ggagcgcgaa accgccgagc
      241 gggttcacgg cttctaccag gaccgctgcc cggtttaccg ctccttgcgg gccgcgatcg
      301 cgatcacgac cgagctcgtc ttcgagccac ttgacggata gttttcggga gttcccacaa
      361 cggatttcgc gctcattgcg tcatcacgag ccccggctgt cttcggctcg ggcgcgataa
      421 tgggccgcgt ggagccgatc gagagagttc gggtggtcgc ccgcgagggg caacgacagg
      481 tcgagcgcga cgacgcgctc gctgtggagg aacccctcga gatccgaatc cgtccggccg
      541 gtccggaccg cccgaaatcc ttcgtcacaa ccatgcggac tccaggaaac gacgaggagc
      601 tcgttgccgg cctcctgttc gcagaaggcg tcgtcgtgtc tcgcgcggac ctgctgcgtc
      661 tcgaaaggcc gctggatccc cgcatcgatc ccgagctccg tgcgaacgtg atgatcgcga
      721 ccctcgcccc ggacgcccag gcgcgcggcg agaagctcga acgcgggacg gtcatgggct
      781 cggcctgcgg ggtctgcggt aagacttcga tcgaaaacgt cctcccttcc gacgcccggc
      841 ctctcgtctc gaacttccgt gtcgacgggg aactcctctt cgcgcttccg gaacggctcc
      901 gggaggggca gtccgtcttt tccaagaccg gtgggctgca cgcagcggct ctcttcggcg
      961 ccgacggacg cctccgggcc ctgcgagaag acatcggccg ccacaacgcg accgacaagc
     1021 tcgtggggca ttttcttctc gaggaccggc tgccgctcgc ggagtcggtc ctgatggtct
     1081 cgggccgcgc gggcttcgag atcgtccaga aggcctatgc ggccgggatt ccgatcctcg
     1141 catcggtgtc cgctccctcg agcctcgccg tctcgctggg cgaggcggcc ggactgacgc
     1201 tggtcgggtt tctgagggag cggcgcttca acgtgtactc gtgccccgag aggattcttc
     1261 cgacctgacg gctcgtccca ctcgagcggt gcccttccgc cgccccgaga aactccttga
     1321 caagacgcgt ccagagttct ggaattgtgc caacgtttgg atttccgcgg aggtgggacc
     1381 atggacctga cccgtcgcgg actcttcaag gcaacggccg tcggaagcgc gatcgccctc
     1441 gggttcgacg tcggccgcgc caaagccgag atgcgcgagt tcaagatctc gcgcaccacg
     1501 gagacccgaa gtatctgtcc ctactgcgcg gtctcctgtg gcgtcatcat ccatacgctc
     1561 ggcgacaggt cgaagaacgc gaaggcgagc gtggtgcacg tcgaaggaga tccggatcat
     1621 ccaatctccc gcggcagtct ctgccccaag ggcatcacgt tgaaggacga catcacgcac
     1681 aagaaccggc tgacgaagcc gaaggtgcgg cgcccgggct ccgacaagtg ggaggacatc
     1741 tcctgggacg aggcgatcgc gaagatcgcg cgccacgtca aggcgacccg cgacaagtgc
     1801 ttcgtcgcga agaacgcgaa gggagaagtc gtcaaccgca ctccgggcat cgcctggatc
     1861 ggcgggtgca ccgacaccaa cgagttcaac ttcctccagt ggaaagtcat cacggccctg
     1921 ggggtgccgt accgggacac tcaggcacgg gtttgacacg gccccacggt ggccagtttg
     1981 gccgccacgt tcggtcgggg ggcaatgaca aacggatggg tcgacatcaa gaatgccgac
     2041 gtcatcctgg cgatgggcgg taaccccgcc gagaatcatc cggtcggttt caagtggttc
     2101 atggaggcga agaagacgcg cggcgcgaag ctcgtcgccg tcgatccgcg cttcacccgt
     2161 accgcggcga cggcggatct ccacgcaccg ctccgcgccg gaaccgacac cgcgttcctg
     2221 ctgggcatca tccgctacgc gatcgagaaa aagcggttcc acgaggacta cgtcaagatc
     2281 catacgaacg cgccgtacat cgtcggcgaa aagttcgcgt tcgacgacgg gctcttctcc
     2341 ggattcgacg aggccaagaa ggaatacgac aagacgcagt ggggctacga gccggatcag
     2401 aagacgacag gctacttcct cgatccgacc ctccagcatc cgcgctgcgt gtttcagctc
     2461 ctgaagaagc acgtcgagcg ctacacgcca gagatggtcg agcggatctg cggaaccccg
     2521 aaggagtcgt acctcaaggt ctgcgagatc gtcacctcga cctatccgcc cgaaaaggtc
     2581 gggacgatca cctacgcgct cggttggaca cagcactcga cggccgtcca gatgatccgg
     2641 gcaggcgcca tgctccagtt gctcctgggc aacgtcggcc ggcccggcgg cggaatgaac
     2701 gcgttccggg gccactcgaa catccagggc gcgaccgaca cagccggaac gttcgacatc
     2761 cttccgggct acctgaagac gccaccagcc tccgcgcagt cgctgaagga ctacctggac
     2821 ggctcgacac cgtcggtcct cggcaagatc gcgtgggcct cgatgaacta ctggtccaac
     2881 tacccgaagt tcatggtttc tttgctcaag actcagtacg gcaaggccgc cactgccgag
     2941 aacgacttcg gctactcctg gctcccgaag gtcgacggca actactcctg gatgtacatc
     3001 tgcgacgaca tgtatcgcgg caattcgacg cgggccggcg gccaggagcc gggacccgag
     3061 ggattcatct ccttcggcat gaatccggtc ggactcgggc cgaactccaa gaagtggatc
     3121 gcagcgcttt cgaaattgaa atggttcgtg gtcgttgaga acgtcgagac ggaggcagca
     3181 gggttctgga aggcgcccaa ggagtacgac ccgcccgatc cctccaagat ccagacggaa
     3241 gttttccttc tgccagccgc gggcttcgcg gaaaaggacg ggacgttcac caactccgca
     3301 cgctggctgc aatggaagtg gaaggcgctc gatcctccgg gccaggcgaa gtcggaccag
     3361 gagatcgtgt cgagaatctt tctggccgtg cgcgatctct acaagaagga ggggggcgct
     3421 ttccccgagg ccatttcgaa cgtcacgtgg agctacagca acccggtcga gccggacctg
     3481 ggcgaggtct tgaaggaaat caacggaaag gcccttcagg acatcccgga tcccaacgac
     3541 aagaccaaga tgctccggac ggcgggccag caggtcgacg gtttcggtca gctccaggcc
     3601 gacggctcga cgacgtgcgg gaactggctc cattccggcg tctacaccga atccggtaac
     3661 aatgcgcagc gacgctcgac ggcagatccg ctcggcctcg gcatgtacca caactgggcg
     3721 ttctcctggc cggcgaaccg ccggatcatg tacaaccgcg cctccgccga cgccgaagga
     3781 aagccgtggg atccgaaacg agccgggatc acctggaacg gcgaaaagtg ggtcggcgac
     3841 gtgcccgaca tgaaaccgga cgcaccgcct ggcacgtacg gcgctttcat catgctgccc
     3901 gagggggtcg cccggctgtt ctcccccggg ctgaacgacg gtcccttccc cgagcactac
     3961 gaggccatcg aggccccgat cgccaaccct ctccacgaga aggtgagctc gaatcccgtc
     4021 agcaagaagt tctcctccga taaggacgtg tacggaaaga gcgaagactt ccccatcgtc
     4081 tgtacgacgt accgtctgac ggagcacttc cactactgga ctcagcacca gcacagcggg
     4141 gttttgaacg agatgcagcc cggcttcttc gtcgagatcc cggagggcct cgcgaaggag
     4201 aagggcatcg cgaacgggtc ccaggtcaag gtgacctccg cgcgcgggtc gatccagggg
     4261 gtcgcgatgg tcaccaagcg gctgatcccc ttgaaggtca acggaaaaga tatgtggcag
     4321 atcgggttcc ccattcactg gggatacgcc ggagatcccg accacaccgg cccgctcgcg
     4381 aacacgctga ctccctcggc gatggacccg aacacgtgga ctccggaata caagtccttc
     4441 ctcgtccgga tcgagaaggc ctgaggggag gggaagatgg cgactcagaa cctcgagatc
     4501 cgccgctcct ccgcttccgt ctggggaagc ctctcgggaa tgcggagcgt ccccaccgtc
     4561 tccaagtaca tcgacacgac gacctgcatc ggctgcaagg cgtgcgaggc ggcgtgcatc
     4621 gaatggaacg acctgcggcg ggtcgcgacc gaacagaccg gcacttacca gacccttccg
     4681 actctccacg cggagtactg gaacctcatc cgcttcaacg agcgcgattt cgacggcggc
     4741 ctcatctggc tgatgcggaa ggaccagtgc atgcactgcg aggagcccgg ctgcctcgcc
     4801 gcctgccccg cgccgggggc gatcgtccag tatgcgaacg gcatcgtgga cgtgaacccg
     4861 gaccagtgca tcggatgcaa gtactgcgag accgggtgtc cattcgacgt gccgcgtttc
     4921 caccagacga ccggcaagat ggcgaagtgc acgctctgcg tcgatcgcgt ctccgtcgga
     4981 ctcgagcccg cgtgcatcaa agcgtgtccg accggctgtc ttcagttcgg cacgaaggac
     5041 gacatggtcg ccctcggact ccgccgggtc gagcaattga agggcaacgg cttcgcacag
     5101 gcgtctctct acaacccgga cggggtgcgc ggcaccggag tcgtgaccgt gctcgcgcac
     5161 ggcgatcacc ccgagtggta ccggctgccc gcggacccgc acgtgccgtg gggcgttcgg
     5221 ttctggaagt cgatcctgcg cccgctcggc gtcgccgcca tgttcggcgc cgtgatcgcc
     5281 gccttcgcgc actacacgaa cttcggcccg aagaaggtca gaggctccgc ggacaccgac
     5341 gcgaacatcc ccagcggaga ggaggtcaaa cgatgagcac cgccgctccc gatcgggcca
     5401 tggatgccgc ggtcatcgcc gggcgcaccc gcgaagacgt cgtcgtcgga aacgagatcg
     5461 tccgtcacaa gcgcgcgtcg cgctggatcc attggaccgt cgccgttacc tttatcctga
     5521 gcctcttgac cggattgccg atctggacgc cgatcttcgg ctggatggcg agcctctttg
     5581 gcggcctgtc ggtctgccgc gtcgtacacc cctgggtcgg catcgcattc ttcgtcatct
     5641 cgggggtcat gttcttctac tggctggcgg acatgcacct gctcccctcc gagcgcgact
     5701 ggctccgccc ggccagggcc attcagtaca tgcgcttcca gaccgacatc gaggacgtcg
     5761 gcaagtacaa cggcggccag aagatcttct tcttcgcggt ctcgctcggc gcgttcggac
     5821 tgctcctctc gggcctgttg atatggttcc cgctcctgtt cccgcggatc atcagcgagc
     5881 tcgccttcgt gatccacgat ttcacgttcg ttctgttcac ggtgggaatc atcgggcaca
     5941 tctacctggg cacggcggcg gagccgggca cgttcgggtc gatggtccgc gggactgtga
     6001 cgcggcagtg ggcgcggctg catcacccga gctggtaccg cgaaatcacg ggtgatgaag
     6061 cccgccgccc ttcatcccct tcgcggtgac gatcattttc ctcgtttatc tcggccatgc
     6121 ggcccggccg gggacgtccg gcgcgatgac gccccgcacg gtcacccgcc catagacgcg
     6181 cctgagccac ccgcgctggt accgcgaagt cacgggtgag aatgccgact gccgctcgcg
     6241 ggtcgaagct cgtttcggaa ttcggccgtc gcgccgcgcg tgccgagctt ctcgcccgcg
     6301 aggccccggc ggccgaggat cccctccggt tcgcggccgg tctttaccgc gttcaatcgc
     6361 gcgtcgtcgc cgggatcgag gcggcggacg cgcaggaacc cttgagcggg aaactcgacg
     6421 acgacgccga tcgccttctc gacgcggcct cctccgtgat ccggtacgcc gcggaagaag
     6481 ggccggacct cctttccgag caggcccggg cccgccagac tgatcttccc tccacggccc
     6541 gaactcgcct cctcgtctac tgggcggcag acagctcttc ggccgaggac tacctctccc
     6601 gagccatcct gcgcccctac gtcgagttcc tgcgctcgat caaccgccca cccgaccgtg
     6661 ttcatgcgcg gggacggtgc cccttctgtg gaggcgctcc ctggatctcc gcccgccgcg
     6721 acgcttccgt gatggagggt gcgcgccgga tgctcggatg cgcactctgc ggcggagagt
     6781 ggtcctttgg ccgcattctc tgtcccgcct gcttcgaggg ggacccggcg aagctccctt
     6841 ccttccagag cgagaagcat cccgcggtgc gcatcgaggc gtgcgagacg tgcaaccggt
     6901 acgtcaagtc gctcgatctt tccgaagacg cccggccgat cccggaggtc gacgacctgg
     6961 tctcgctctc gatggatctc tgggccatgg agtccggctt tacacggatc gagccgggcc
     7021 tcgcaggtct gtagcttgac gtcacccgac gggctgatcg ccgcccagag tggactccgc
     7081 agtcgcttcg aggacttccg ccatgctctc gaccgtcgag acgaggcagc cctccggctc
     7141 gctctgatgg atttccacgc ccatctcctt ctgtggacca cggccgagga acgagtcctg
     7201 ctgcccgcgc tcgagcgcac gaccttcccc ggccgcgacc ctcaacgcga gctcaagctc
     7261 gagtacgtgc agatccggga gctcacccgc taccttctct cccagatcga ggggcatgcg
     7321 ccgatcgcgg acgtgctcgg cctggtcgag aatctcgatc gaaggctgtc cgcccacgaa
     7381 tccgaaatgg agaaggtcta ctacccctcc gccgcgccga ttctgacgtc ggaggaatgg
     7441 cgtcttctga cggaggccag cccgcctccc tgacgtcctc agcccagctg ctggatttcg
     7501 aacgccggct cgtccgggcg tggccgaagc gcctcgtagg ccgttttcag ggcttccggg
     7561 ctccagccga gaaaaacgcc ctcaccgcgg taccagggct ccccggtcgc ggcttctacc
     7621 agcacgatcg caccctcccc ttcgtcgctc tccacggaca aacgccagcc cgaaagcgtc
     7681 acgccgcttt cccgggtgcg aatcggagcg cgcctgagca acaccgtgcg ggagttcgtc
     7741 aggccgggca ccggcggaag ggcaactccc ccgccggaag cgccttcgct catcgcgtca
     7801 gcccccccga gtgtgcattt cgagatgagg attctggcgc agcgcactcg ccgtgagcag
     7861 aggcgccctc ttgcgcagca acagccgctc ctccgctcca cgagccgtgc gcgcgtgcca
     7921 agcgtcttcg agaagcgccc gcagctccga ctccgacgct ccggcgcgga gcgcacctcg
     7981 caggtcggtg ccccgggcgg catagagaca gaggtaccac atcccgtccg cggtcaaccg
     8041 gctgcggtcg caggagccgc agaacggagc ggtcgtcgaa gaaatgactc cgaagacggt
     8101 tccgtcggcg agacggaatc ggtcggccgg agcggatgtc tcctcgcgca gcgggacgat
     8161 cggcccgtag tggcgggcga ggctcccgag catctccgcg cgcgtgacca cgcggtccat
     8221 cgaccagcgg gtggctcctc cgacgtccat gtattcgatg aaccggactt cggcgccgat
     8281 gcgccggccg tactcgagaa ggggtaccag ttcgtcctcg ttgactccac gaatcacgac
     8341 ggcatcgacc ttcacggcgg gaaaaccgac ctcggacgcc gcggcgatcc cggacagcac
     8401 acgctcgagc tcatcggacc gcgccagctc ccggaagcgc tccgggcgga gcgtatcgag
     8461 gctgacattg acgcgatgaa gccctgcggc gcgaagagcg gcggcgtgtt cttccagaag
     8521 cacgccgttg gtcgtgagcg cgagatctcg aattccggac ttcgaggcga ggaggcgcac
     8581 gagctcctgc aggttttgac gcacgagcgg ctcgccgccg gtcagcctca cacgatcgac
     8641 gcccaggccg atgaacacgt cgacgaggcg gctgacctcc tcgaaatcga ggatgtccga
     8701 acgaggaagc cagacgtagt ccttctcggg catacagtac tgacagcgca ggttgcagcg
     8761 gtcggtgacc gaaacccgaa ggttacgaag cggccggcgg aacgtgtcga gcagggccac
     8821 ggcccaattc tacgtgccac gcctcatccg gcgcgcttcc accggtcgaa gattcgatcg
     8881 atctgccgct cggcatcccc ggcggccgcc tccggcgtga cctctttccg cgcgacgcgc
     8941 gcgaacatcg tcggaatgac gaacgtgttg aagacctcgt cgatcgccgc ggtcgcgtag
     9001 ccgggaaagc cgacgttggt cgcccagtcg aggaccgaac cgagcaccct gtacttgtcg
     9061 ggcgggtcgg ccttcggatc gctcgagagc ctctgctgca ggtccggaag cgagccgggg
     9121 aagctcggga agttgtacga ctccccctcc cggaatacgt cggagaagtg gtcgacgagg
     9181 tccacgagga actgcttcgc caggtccggg ttttccgcga atttccagat cacgtagcag
     9241 ttcatgacgt gctcggacgc gatccggcga acgggtcccg cgagcgccga cgtcagcccg
     9301 atcttcttcg acatctccgg gttttcgcgc tcggccgtcc gggtgacgga gatcgcgttg
     9361 cacacgaagg aggctcgccc cgcgaggatc atccggttgt tggacgacgg gtcccaggtg
     9421 aacacctccg gggtcatcgt ctcctcgtag agcgcacgga cgaacttcaa tgcttcgatc
     9481 gtccccctcg agttgattgc gacgcgcccc tcctcgtcct gctccgcgcc gccgaaagac
     9541 caaaggaccg cgcgcagcgc catgttcgag tcgatctcct gcgagagacc gatccccacg
     9601 gaattcccga actccttctt gatccgagcg ccaccgaccc ggaggtcctc ccacgaatcg
     9661 gggcccttcg gaaaaccgac gctccgccac aggtctgtcc ggtagttccc cggatccggc
     9721 acgtaggaat cagagaacgc gaagtacttg cccgttttcg gattgaacgt cgaccgctcg
     9781 gccagcgaaa tcatcttccc gtgcttcttc tcgacggcct cgactacctc gcgatggtcg
     9841 atgacctgcg attcataggc ggccggcggc gagagaaaga gaaagaggtc gtgtcccttg
     9901 ccggccgcgg cctccgacgc tccgcgtgcg ggtacctccg tcgacgccat gtggtccacg
     9961 gtgaccgtga tgccgttctt ctcgccccac gccctcgtgt agaccccgtc gaaccagcgg
    10021 tcgtatgccg ggatgaagtg actccactgg aggatccgga gagttttccg gccggccgac
    10081 ggctgcttga cgcggcgtcc gcaggcgaga gcggggagag cgccgagagt cagcgcggcg
    10141 ccggcgctct tgacgagctc gcgacgcgag agcctccgcg cattcgtcga cctcattctc
    10201 tcctcgagtg ccggtcgtct cgtcgccgat ccgaacgaag cggccgaagt ctataatccg
    10261 ggtccgaaag gctccggcgg gtccggagcg ggaggttttt tggcggccct cgaacccggc
    10321 gcaatgtttc cgcagatcga gctcgaaacg gagtctggcg cccggttcgt tcctcccgcc
    10381 gccgagaccc tgtacgtctt cttcaagacc acttgcccga catcggagct ctcgtggccg
    10441 catctcgaaa ggatccgccg catcgcctcc acagcgctcc cgatcttcgc cgtctcgcag
    10501 gacgagcgac cggagacggc ggcgttcaac gatcgcctcg gcgtccacgt cgagacgctc
    10561 tacgatccct ctccatggaa agcgtctgcc gagctcggtc tgaccaacgt gccgacgttt
    10621 tttctcgtcg ctcccggcgg ccggatccgc gagacggtcg tcggctttca gagaccgaag
    10681 ctcgaggcgc tcgcgaaacg cgccggaaag ctctcggggc gccggtccgt ccggctcttc
    10741 ccgatcggcc agaacgtccc tctcatcgtc cccggatgag agtcacgcaa cgcgggctgg
    10801 tagcctgcag gtgagggcga tgccgaggcc ggggaacctg ggagagctga aagcgagcgg
    10861 ctaccgtccg cgcggcgtca aggaagagct gcgcggcaac ctgcgccgcg cgctccgcga
    10921 ggggaagaac ccgttcgagg ggatctacgg ctacgagcgc acggtgttgc cggcgctcca
    10981 caacgcggtc ctttcgaaac acgacttcct cctcctgggg ctgcggggcc aggcgaagac
    11041 ccgcctgctg cgccttctcg tcggcctgct cgacccggaa atcccggcga tcgccggaag
    11101 cgacctgaac gaggacccgt tccgcccgat cacgaagtac ggccgccgga tcctttcgga
    11161 acgcggcgac gacatgccca tcgagtggat ctcgcgcgag gagcgctacc gcgaaaagct
    11221 cgccacgccc gacgtcacca tcgcagacct gatcggcgac gtggacccca tcaaggcggc
    11281 gaccgagcga cgctcctact cggacgaaga ggtcatccat ttcggaatca tcccgcgcac
    11341 gaaccggggc atcttcgcga tcaacgagct gccggacctg cagccgcgca tccaggtcgg
    11401 cctgctgaac atcctcgaag agcgcgacct ccagatccgc ggcttcccga tccggatccc
    11461 gctcgacatc ctgctcgtgt tctcggccaa tccggaggac tacaccaacc gagggaacat
    11521 catcacgccg ctgaaggacc ggatctcgtc gcagatcctg acccactacc cggacgacgt
    11581 gaagaccgcg gcacgcatca cggcgcagga ggcgtggctg gatcgcgatc cggcgcccgc
    11641 cgtcgagatc cccgagttct tccgggaact ggtcgaggaa atcacgttcg cggcgcgcac
    11701 gagcgagttc gtcgaccagt cctccggcgt ctcggcgcgg atgccgatct cagcggtcga
    11761 gatcctggcg tcgaatctcg agcgccgcgg cctcgtccac ggggagcgcc aggtctttcc
    11821 gcgcctctgc gaccttcccg cgtgcctccc ggcgatcacc gggaaagtcg agatggtcta
    11881 cgagggagag cagcagggcg ccgaagtcat cgcaaagaag ctgatcgcga cagcggtcaa
    11941 aaaactgttc gattcgcgtt ttccgccgcc ggactccggc cgcccgacgc agcgccgccg
    12001 tgccgacgag gacgacaccg acgagctctc gatgccgcgc cgcacgcgct cgactccccc
    12061 gcccgagaag atccacccgc tctacgagcc aatcctcgag tggttcgctt ccggcaacaa
    12121 gatcacgctc tccgacgaca cgccgttttc cgagcacctc gaaacgctct catcggttcc
    12181 gggattgaag aaggcggtgg aagagcactt cgcacccgcc tcgaaagagg agctggcggt
    12241 cggaatggag ctcgtcctgg aaggcctgac tcagcatctg aagatcgccc gcgaggacct
    12301 cgactcgcgg gtgtcctacg gcgagatgct gaagttcaac ctggtgcgga gccggaccac
    12361 ctaggagctg tcagcggccg cggctcgtcg ggacccccag ccgcgcggcg atgaacgccc
    12421 acatgtccga tcgttcggcg atctgctccc gtctcgacgc gtacccgtgg cttgcgtcgc
    12481 tcgacacgcg aacgaggatg ggccggtcgc acccctgcgc tgcctgcagt gcggcggtga
    12541 acttgtagga gtggctcggc accacgcggt cgtcgtggtc ggccgccagg agcagcgtcg
    12601 ccggatagca cgcgcctttc cgcacgtttt gcagcggcga ataggcgaag agccaccgga
    12661 atgccgccgg ttcgtcggac gatccgtact ccgggatcca cgcggagccg acggtgaacc
    12721 ggtggtagcg caacatgtcg tagtgaccgg cgttcccgat tgccacgccg aagagatctg
    12781 ggcgctcggt gctgacggcg ccgatcagca ggcctccgtt cgagtgcccg tagatcgcga
    12841 gtttcgcggg agtggtgtat cgctcggcga cgagatactc cgcggcggcg atgaaatcgt
    12901 cgaagctgtt ctgcttcctt tcgagcattc ccgcccggtg ccagtcctct ccgtactctc
    12961 cgcctcctcg taggttcgcc agcgcgtaga tgccgcccat ctccaaccag agagggatat
    13021 cgggctcgta ggcggggccg aggctcgcgc cgtatccgcc atatcccgtc aggaaaacgg
    13081 gattggagcc gtcccgccgc aatcccttct tcgaggtgag aaacaacggc acgcgcgtgc
    13141 cgtcctttga agtgacgaag acctgtcggg tctcgtcggc gttcgcgtcg aaggggactc
    13201 ggggcggtcg gaacggagtg ctcaccccgg tcccgggatc gtaatggtag acggtcgtag
    13261 ggagcaggaa cgaacggaag ctgtagaaga acgagggcgt cgacccaccc tcgaaccaac
    13321 cgatcgctcc cacacccggc agagcgacct cacccgcggg cttcccgtcc aacgtgaaga
    13381 gtcgcagccg gctctgcacg tcggcgaggt actgcagcac cagccgatcg ccggccacga
    13441 cggcccgttc gatcgtgtcg gaggattccg ggacgaccgt ccgcggagtc gccttcgccc
    13501 cctcctccat gtcgagcgcg atcacgcgat agcgaggcgc gtcgagatcg gtgcgcacgt
    13561 aaaggaggtt cccggaagat cccatcggct gataaccggc ctcctggttg cccagcaggc
    13621 gcaccagcgg tgccgtgaga tcgggatgct ccgggtctcg aaggtccatc gttcccaccc
    13681 atccgcctcc cgccttctcg cccaccgcga tggcgcgcct tccgtcgttg ctcaacatgc
    13741 agtagatcca ggc
//
LOCUS       DASWAV010000051         4093 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U2665
            k141_281990, whole genome shotgun sequence.
ACCESSION   DASWAV010000051 DASWAV010000000
VERSION     DASWAV010000051.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960316
            Sequence Read Archive: SRR10260007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 4093)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4093)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:47:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,929
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4093
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_281990"
                     /isolate="SMAG_U2665"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.36444 N 132.31889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..276)
                     /locus_tag="VF958_01165"
     CDS             complement(<1..276)
                     /locus_tag="VF958_01165"
                     /inference="COORDINATES: protein motif:HMM:NF016693.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEX9147746.1"
                     /translation="MDSGEPGYDYDAVVIGGAVAGASTAILLRRRVPGARVLVVEKSD
                     QFDWKVGESTVEVSAYFLTRVLKQYDHLSREQLPKQAFRYWFSNSDVT"
     gene            complement(286..2466)
                     /locus_tag="VF958_01170"
     CDS             complement(286..2466)
                     /locus_tag="VF958_01170"
                     /inference="COORDINATES: protein motif:HMM:NF024907.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRTAC1 family protein"
                     /protein_id="HEX9147747.1"
                     /translation="MNRRTVILSAVLLLLLGAAGAWFYRNAYVPRLRREFIPWMERLG
                     AWRDGVEQVQRALKSREATDDDPYGIAPLRSWAWSGLDRVAWVTYEVEKPRHDVSNAS
                     DTTRGGVLFKVGGFRLDGARVERSSWVMVTVEWRVDVTVRLTRVLSERVEPHPRFRNA
                     TAETGLGASRHDPPGPGTNRLIAGIWPGSGVAVLDFDGDGDEDLFVGDGVRSILYEND
                     GTGRFTDVTERAGLARSAEEGVAATGVAAADVDGDGRPDLFVTNSFGPGRLFRNVDGR
                     RFSDVTAESGIRTPPNARSAAFADVDGDGDLDLFVCVTGDYYRQMPDPPYDAEDAGSN
                     LLFVNDGHGRFRDASADWGMSRPKRWSLSSLFADYDGDGRPDLLVTNDFGLKNLYRND
                     GGRRFVDVTDRAGVADRAYGMSGAWGDFDGDGRLDLYTTGCFTQWFFLHEYPGLPIPF
                     PGRLFLSTAVTWMEKMTRGNSLLLQRADGRFEDATNDSGARKAGWNWSAVAADLDDDG
                     WLDLYATNGMWGDGRDHDRELEFWWETLAYWNDYVALKKTFDRKGAGIAGIERDRYFH
                     NRHATRGAGDPLFVDQAFLDGLDLETNGRAVIAFDANGDGALDLYVRSVQAPEALFLG
                     SRGKDEHFLRVRLIGSPGKDNSQGIGARVVATLPGGRKILRENGNASGYLSTASPVVH
                     LGLGAAARLDDLTVRWPSGKLQTLGPVTAVDRAIVVDEERGALP"
     gene            2732..2824
                     /locus_tag="VF958_01175"
     tRNA            2732..2824
                     /locus_tag="VF958_01175"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2766..2768,aa:Ser,seq:gct)
     gene            2843..>4093
                     /locus_tag="VF958_01180"
     CDS             2843..>4093
                     /locus_tag="VF958_01180"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HEX9147748.1"
                     /translation="MPLSPGTRLGPHEIIEPLGSGGMGEVYRARDTRLDRMVAVKVLP
                     AHLTDNDELRQRLEREARVISQLSHPHICALYDVGHQDGVDYLVMEYLEGETLTDRLG
                     RAPLPAEQVLRYGVQIADALDKAHRQGIVHRDLKPGNVMITKSGVKLLDFGLAKFRAV
                     GSDGAFSAASVLPTRTDASLTAQGTILGTFQYMAPEQLEGSEADARSDIFALGAVLYE
                     MATGKKAFAGKSQASLIAAILDATPPPISTVQPMTPPALDRVVKTCLEKDPEDRWQTA
                     HDVMLELKWIAEGGSQAGLPASVVARRRSRERLAWIVASILAASTAALGVLELKRPRA
                     APSIVRSSILSPDKTTFSFVGGGAGPVVISPDGRQLAFVALDTGGKRRLWIRSLETLL
                     AKPLPATEGATYPFWSPDSRFLGFF"
BASE COUNT          675 a         1386 c         1350 g          682 t
ORIGIN      
        1 ggtgacgtcc gagttggaga accagtatcg gaaggcctgc ttcggcagct gctcgcgcga
       61 gaggtggtcg tactgtttga ggacgcgcgt caggaagtag gcggagacct cgaccgtcga
      121 ctctccgacc ttccagtcga actggtccga tttctcgacc acgaggacgc gcgcccccgg
      181 cacacgccgc ctgagcaaaa tcgccgtcga tgcgccggcg accgcgccgc cgatcacgac
      241 ggcgtcgtag tcgtacccgg gctcgccgga gtccatgtcg agagtctacg gcaaggcgcc
      301 ccgctcctcg tcgacgacga tcgcgcggtc gacggcggtc accgggccga gcgtctgcag
      361 ttttccggac ggccagcgta ccgtcaggtc gtcgaggcgc gcagccgcgc cgaggccgag
      421 gtgaacgacc gggctggcgg tcgagaggta accgcttgcg ttgccgttct cgcgaaggat
      481 cttccgcccg ccgggcagcg tcgccacgac cctcgcgccg atgccttgcg agttgtcctt
      541 tcccggtgac ccgatcaggc ggacgcgcag gaagtgctca tctttcccgc gcgatccgag
      601 gaagagcgct tcgggtgcct gcaccgaccg tacgtagaga tcgagcgccc cgtcgccgtt
      661 ggcgtcgaaa gcgatgaccg cccggccgtt ggtctcgaga tcgagcccat ccaggaacgc
      721 ctggtccacg aagagcggat cacccgcccc ccgggtcgcg tgcctgttat gaaaatagcg
      781 gtctcgttcg attccggcga tgccggcgcc cttgcggtcg aaggtcttct tcagcgcgac
      841 gtagtcgttc cagtacgcga gcgtctccca ccagaactcg agctcgcggt cgtggtcgcg
      901 gccgtcgccc cacatgccgt tcgtcgcgta gaggtcgagc cagccgtcgt cgtccaggtc
      961 cgcagcgact gcgctccagt tccagcccgc cttgcgcgcg cccgagtcgt tcgtcgcgtc
     1021 ttcgaagcga ccatcggccc gctggagcaa gagcgagttc ccgcgggtca tcttttccat
     1081 ccacgtaacg gcagtcgaga gaaacaggcg tcccgggaac ggaatcggca gacccgggta
     1141 ttcgtgcagg aagaaccact gcgtgaagca cccggtcgtg tagagatcga gtcggccgtc
     1201 gccgtcgaag tccccccatg cgcccgacat gccgtacgcg cgatcggcga cgccggcgcg
     1261 atcggtgacg tcgacgaacc tgcgcccgcc gtcgttgcga tagagattct tcaggccgaa
     1321 gtcgttcgtg acgaggaggt cggggcggcc atccccgtcg tagtccgcga agagggacga
     1381 gagagaccag cgcttcgggc gcgacatgcc ccaatccgcc gaagcgtcgc ggaagcggcc
     1441 gtgtccgtcg ttgacgaaaa gtagattgga gccggcgtct tccgcgtcgt agggcggatc
     1501 gggcatctgc cggtagtaat cgccggtcac acagacgaag agatcgaggt cgccgtcgcc
     1561 atccacgtcg gcgaatgcgg ccgaacgcgc gttcgggggt gtccgtatcc cggactccgc
     1621 ggtgacgtcg gaaaatcttc ggccgtcgac gttgcggaag agcctccccg gtccgaacga
     1681 gttcgtcacg aacagatcgg gccgcccgtc gccgtccacg tcggctgccg cgacgccggt
     1741 tgcggcgacg ccttcctcgg ccgagcgcgc gaggccggcg cgctccgtca cgtcggtgaa
     1801 ccggccggtg ccgtcgttct cgtagaggat cgagcgcacg ccgtcgccga cgaagaggtc
     1861 ctcgtcgccg tccccgtcga agtcgagcac cgcgacgccg gaccccggcc agatccccgc
     1921 gatgagacgg ttggtgccgg gcccgggcgg gtcgtgccgg ctcgcgccga gcccggtttc
     1981 cgcagtcgcg tttcgaaagc ggggatgcgg ttcgactctt tcagagagta cgcgggtgag
     2041 gcgaacggtc acgtcgactc tccattcaac ggtcaccatc acccacgacg atcgctcgac
     2101 ccgcgccccg tcgaggcgaa aaccgcccac tttgaagagg acaccgcccc tcgtcgtgtc
     2161 gctcgcattg ctgacgtcgt gacgcggttt ctcgacttcg taggtcaccc aggcgacccg
     2221 gtcgagaccc gaccaggccc agctcctgag cggcgcgatg ccgtagggat cgtcgtcggt
     2281 cgcctcccgg ctcttgagcg cccgctgaac ttgctcgacg ccgtcccgcc aggccccgag
     2341 ccgctccatc caggggatga actcgcggcg gagccgggga acataagcgt tgcggtagaa
     2401 ccaggcgccg gcggcgccca ggaggagaag caggacagcg gagaggatga cggttcggcg
     2461 gttcaacggc gggatgttag cgtgttcgat ggaccgactg ggatcagaac cagcggtttc
     2521 ggcgtttctc cgcctcgggc gtcatctcgt aacatgcgca gccaaggccc taccggagag
     2581 gtgggtgagt ggctgaccga ccggaggagc gcgcagcgct ttcggtcggg gcgccggctt
     2641 tgccggcgcc aacccggcgg gtgcggcgaa gccgcccgct ggtttgttcc tcgagcgcca
     2701 tcccgtaaca tgcgccctta aggccatccc cggagaggtg ggtgagtggc tgaaaccagc
     2761 ggtttgctaa accgccgtag ggctaataac cctaccgccg gttcgaatcc ggccctctcc
     2821 gccatttaga atcgatcctt ctatgcccct ttcccccggt actcgcctcg ggcctcacga
     2881 aatcatcgaa ccactcggct ccggcggaat gggagaggtc taccgcgcac gcgatacccg
     2941 gctcgatcgc atggtcgccg tgaaggtcct ccccgcacat ctcaccgaca acgacgagct
     3001 gcgccaacgt ctcgagcgcg aggcgcgcgt gatctcgcag ctttcccatc ctcatatctg
     3061 cgctctctac gacgtggggc accaggacgg cgtcgactac ctcgtcatgg agtacctcga
     3121 aggggaaacg ctgaccgacc gtctgggaag agcgcctctt cctgccgagc aagtgctccg
     3181 ttacggggtc cagatcgccg acgctctcga caaggcccac cgtcagggaa tcgtgcaccg
     3241 cgacttgaag ccgggcaacg tcatgatcac gaagtcgggg gtgaagctgc tcgacttcgg
     3301 gctcgcgaag ttccgagcgg tcggttcgga tggcgcattt tcggccgcgt ccgtccttcc
     3361 gacccggacg gacgcgagtt tgacggcgca gggcacgatc ctcgggacct tccagtacat
     3421 ggcgcccgag cagctcgagg gatccgaagc cgacgcccgg agtgacatct tcgcgctcgg
     3481 tgccgtgctc tacgagatgg cgaccggaaa gaaagccttc gccggcaaga gccaggcgtc
     3541 cctgatcgcg gcgatcctcg atgcgacgcc gccgccgatc tcgacggtcc agccgatgac
     3601 gcctcccgcg ctggaccgcg tcgtcaagac ctgcctcgaa aaagatccag aagaccgctg
     3661 gcagacggcg cacgacgtga tgctggagct caagtggatt gccgaaggag gctcgcaggc
     3721 cggcctgccc gcatccgtcg tcgcgcggcg gcgaagccgt gagcggctcg cgtggatcgt
     3781 cgcgagcatc ctcgccgctt cgacggcggc gctcggagtt ctcgaactca agcggccccg
     3841 cgctgcaccg tccatcgtcc gctccagcat cctgtcgccg gacaagacga ctttcagttt
     3901 cgtcggcggt ggagcggggc cggtcgtgat ctcgcccgac ggccggcagc tcgccttcgt
     3961 cgcgttggac accggcggca agagacgtct ctggattcgg tccctggaga cgttgctggc
     4021 gaaacccctg ccggcaacgg agggagcgac gtatcccttc tggtctcccg acagccgatt
     4081 cctcggcttc ttc
//
LOCUS       DASWAV010000052         3141 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U2665
            k141_2703, whole genome shotgun sequence.
ACCESSION   DASWAV010000052 DASWAV010000000
VERSION     DASWAV010000052.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960316
            Sequence Read Archive: SRR10260007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 3141)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3141)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:47:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,929
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3141
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2703"
                     /isolate="SMAG_U2665"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.36444 N 132.31889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..434)
                     /locus_tag="VF958_01185"
     CDS             complement(<1..434)
                     /locus_tag="VF958_01185"
                     /inference="COORDINATES: protein motif:HMM:NF019013.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ester cyclase"
                     /protein_id="HEX9147749.1"
                     /translation="MKKAALLLLAGVVVFSASLSAESAEKRKAVARRVFEEIFNQGRF
                     EVADEIYAKDFVNHGATRDIGLKEDQEAARGWRSAFPDLKMKVDKTLVDGEFVTVLWS
                     GEGTNTGEGNGLPATGKTLKGRGITIWRISGGKIREEWSEFD"
     gene            complement(461..>3141)
                     /locus_tag="VF958_01190"
     CDS             complement(461..>3141)
                     /locus_tag="VF958_01190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HEX9147750.1"
                     /translation="IAVDPKDSNVVFVCATGHLFDDHPDRGVYRTKDGGKTWDKVLFV
                     ANDTGCSDVAIDPSDGRTVYAGMWQFRRKPYFFSSGGPKSGLYRSTDGGATWRKVSKG
                     LPPGDLGRIGLTVSPVQPEVVFASVESRRDAKVPGSEDSSFYRSGDRGETWVRQSTAS
                     PTTGRPFYFSNMAADPKNADRVYKTTYNLHVTDDAGKSWSALGGNYHGDTHAVWINPK
                     NTEELLVGTDGGVYHSADRGAHWRFVSSLPVAQFYHVSSDSADPYNLYGGLQDNLVWY
                     GPSRYPGEISPSRWTSLLFCDGFWTFVDPTDEDFVYCECQGGWIFRTRKSTREIKDIK
                     PAPKEGEPKYRFNWNAPIHISATQPGTIYLGGQFLFRSRDRGESWERISPDLTTNDRE
                     KQKQEESGGLTRDNSTAENHCTIFAISESPKNKDVIWTGTDDGNLQITRDGGKSWTNV
                     AGNVTGLPKGTWVSRVEASTYEEGTAFATFDGHMTGDMKTWVYRTADFGKTWKSLATA
                     DLKGYAHVVKQDPVNPNLLFLGTESGLFVSLDGGASWAAFRAGMPAVAVRDLEIQRRE
                     GDLLIATHGRGIYILDDLDPLRALTPKVLESDVAFLPTRPAALAIPASPEVGWSGDGN
                     FSGRSLPELATITYYLKKRHIIGDLKLEISDPDGKLVSTINGGKRKGINRVEWAMRGS
                     TPRYAPGTGQIPSFASIVGPRVLAGTYKVKMIKGKDTYSTDLKVVYDRRSRVSDADRK
                     VQSDTAWKLHGLVEGLTVLVAQITDARDQSRARAEGLPANDALRKKLEALSDELEAQR
                     TALVSTAQGEGISGEEKLREELGMLYGNVNGSEERPTASQLARTGVLAKDLDSARAKY
                     EGSLKKLIPLNPELEKRKLMPIQPVTEEEWRKKTAA"
BASE COUNT          454 a         1042 c         1038 g          607 t
ORIGIN      
        1 tggtcgaact cgctccactc ctcgcggatc tttccgcccg aaatgcgcca gatcgtgatg
       61 ccgcggccct tgagcgtctt tcccgtggcc ggcaggccgt tgccctcacc ggtgttcgtt
      121 ccctcgccgc tccagaggac cgtgacgaac tcgccgtcga cgagcgtctt gtcgaccttc
      181 atcttgagat cgggaaaggc ggaccgccag cctcgcgccg cctcctggtc ctccttcaac
      241 ccgatatccc gggtcgcgcc gtggttgacg aagtccttcg cgtagatctc gtcggcaacc
      301 tcgaaccggc cctggttgaa gatctcctcg aagacgcggc gggcgacggc ctttcgtttc
      361 tccgcggatt cggccgacag cgaggcggag aacacgacca cacccgcgag caggagcaac
      421 gcggcctttt tcatttcgag catccttccg gttcgaagac ctaagccgcc gttttcttcc
      481 gccactcctc ttcggtcacc ggttgaatgg gcatgagctt gcgcttctcg agctcggggt
      541 tcagagggat gagcttcttg agtgagcctt cgtacttcgc gcgggctgag tcgaggtcct
      601 tcgcgagaac acccgtgcgc gcgagctgcg acgcggtcgg gcgctcttcc gagccgttca
      661 cgttgccgta gagcatcccg agctcctcgc gcagtttctc ctcgcccgag atcccttcgc
      721 cctgcgccgt cgagacgagc gcggtccgct gcgcttccag ctcgtcggat aaggcctcga
      781 gcttttttcg aagggcatcg tttgccggca gcccctccgc ccgcgccctc gactggtcgc
      841 gcgcgtcggt gatctgcgcg acgaggacgg tgagcccctc gactaaaccg tgcagcttcc
      901 acgccgtgtc gctttgcacc ttgcggtcgg cgtcgctcac gcggcttcgc cggtcgtaga
      961 cgaccttcag gtcggtcgag taggtgtcct tgcccttgat catcttcacc ttgtacgtcc
     1021 cggcgaggac ccgcgggccc acgatcgagg caaagctcgg gatctggccg gtgcccggcg
     1081 cgtatcgggg cgtggaccca cgcatggccc actcgacgcg gttgatcccc ttgcgctttc
     1141 ctccgttgat cgtcgagacg agctttccgt cggggtcgga gatctcgagc ttcaagtcgc
     1201 cgatgatgtg gcgcttcttc aggtagtagg tgatcgtcgc gagctccggc aggctgcggc
     1261 ccgagaagtt gccgtctccg ctccatccga cttccggcga cgcggggatc gcgagcgccg
     1321 cgggccgcgt tgggagaaac gcgacgtccg actcgagaac tttcggagtg agcgcgcgca
     1381 gagggtcgag gtcgtcgagg atgtagatcc cgcggccgtg ggtcgcgatc aggaggtccc
     1441 cctcccgccg ctggatctcg aggtcgcgga cggcgacggc gggcatcccc gcgcggaacg
     1501 ccgcccagga ggcgccgccg tcgagagaga cgaagagccc gctctcggtt ccgaggaaga
     1561 ggagattcgg gttgaccggg tcctgcttga cgacgtgggc gtagcccttg aggtcggcgg
     1621 tcgcgagcga cttccaggtc ttgccgaagt ccgccgtccg gtagacccag gtcttcatgt
     1681 cgccggtcat gtgcccgtcg aaggtggcga acgccgtgcc ctcctcgtac gtcgacgcct
     1741 cgacgcgcga gacccacgtc cccttcggga ggccggtgac gttgccagcc acgttcgtcc
     1801 agctctttcc gccgtctcgc gtgatctgca ggttgccgtc gtccgtgccc gtccagatca
     1861 cgtccttgtt cttcggggac tcgctgatcg cgaagatcgt gcagtggttc tcggcggtgg
     1921 agttgtcgcg ggtgaggcct ccggactcct cctgcttctg cttctcccgg tcgttcgtgg
     1981 tcaggtccgg cgagatgcgc tcccagctct ctccccgatc gcgcgagcgg aacaggaact
     2041 ggccgccgag atagatcgtg cccggctgcg tcgcggagat gtggatggga gcgttccagt
     2101 tgaaccgata cttcggctcg ccctctttcg gcgctggctt gatgtccttg atctcccggg
     2161 tcgacttccg cgtgcgaaag atccagccgc cctggcactc gcagtagacg aagtcctcgt
     2221 cggtcgggtc gacgaacgtc cagaagccgt cgcagaagag gagcgacgtc caccgcgacg
     2281 gagagatctc gccggggtag cgcgaggggc cgtaccagac gaggttgtcc tggaggccgc
     2341 cgtacagatt gtagggatcg gccgagtcgg agctgacgtg gtagaactgc gcgaccggca
     2401 gagaagacac gaaccgccag tgcgcgccgc ggtcggccga gtgatagacg ccgccgtcgg
     2461 tgccgacgag cagctcctcc gtgttcttcg ggttgatcca gacggcgtgg gtgtcgccgt
     2521 ggtaattgcc gccgagcgcg ctccagctct ttcccgcgtc gtcggtcacg tggaggttgt
     2581 aagtcgtctt gtagacgcgg tccgcgttct tcggatcggc cgccatgttg gagaagtaga
     2641 acggccggcc ggtcgtcggg ctcgccgtcg attggcgcac ccaggtctcg ccccggtcgc
     2701 ccgaccgata gaagctcgag tcctccgagc cgggcacctt cgcgtcgcgc cgggattcga
     2761 cggacgcgaa cacgacttcc ggctgcaccg gcgagaccgt gaggccgatc cggcccaggt
     2821 cccccggcgg aaggcccttc gagacctttc tccacgtggc gccgccgtcg gtggatctgt
     2881 agaggccgct cttcggaccg ccggagctga agaaataagg cttgcgccgg aactgccaca
     2941 tcccggcgta gaccgtccgg ccgtcggacg ggtcgatcgc gacgtcggag cagcccgtgt
     3001 cgttcgcgac gaagagcacc ttgtcccacg tcttgccgcc gtccttcgtc cggtagacgc
     3061 cgcggtccgg gtggtcgtcg aagaggtgtc cggtggcgca gacgaaaacg acgttcgaat
     3121 ccttcgggtc gacggcgatc c
//
LOCUS       DASWAV010000053         3649 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U2665
            k141_122753, whole genome shotgun sequence.
ACCESSION   DASWAV010000053 DASWAV010000000
VERSION     DASWAV010000053.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960316
            Sequence Read Archive: SRR10260007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 3649)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3649)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:47:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,929
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3649
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_122753"
                     /isolate="SMAG_U2665"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.36444 N 132.31889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(303..638)
                     /locus_tag="VF958_01195"
     CDS             complement(303..638)
                     /locus_tag="VF958_01195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9147751.1"
                     /translation="MSRAARVAAILVLAVILLPALVSACPLCKDAEADTPGGTRGFGL
                     GIYYSILLMIAVPWTAVGTVAFLIFRKRRRLGKVAGTGPAGLHPLQSSTLPEAAVGRF
                     VPDSRGARS"
     gene            complement(649..1059)
                     /locus_tag="VF958_01200"
     CDS             complement(649..1059)
                     /locus_tag="VF958_01200"
                     /inference="COORDINATES: protein motif:HMM:NF016153.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF420 domain-containing protein"
                     /protein_id="HEX9147752.1"
                     /translation="MNATLNALSAVFLLIAYRAIRGRQIERHRRWMLTAASTSALFLV
                     SYVAYHARVGSVRFAGTGAARTVYFAILGSHTLLAMVVLPLVLRTLYLGLKRRDDRHR
                     RIAKWTFPIWLYVSVTGVVVYLMLYHLYARPAIR"
     gene            complement(1094..1693)
                     /locus_tag="VF958_01205"
     CDS             complement(1094..1693)
                     /locus_tag="VF958_01205"
                     /inference="COORDINATES: protein motif:HMM:NF012720.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme-copper oxidase subunit III"
                     /protein_id="HEX9147753.1"
                     /translation="MSSHAIAHDHALEPPSATGIPHDKMGMWVFLCSEVMFFTGLIGS
                     YIVLRFASPSWPHPGNTLNIWLTALNTFILICSSVTMVKALESVRNGDLGKLKLFLSM
                     TLLGGAIFLSIQVYEYRHLLHAGFNPHVSLFGSVFYTTTGFHGFHVFCGVVCMAFVTG
                     KAFLGKYTQAHHQGVETIGLYWHFVDLVWIILFTIIYLF"
     gene            complement(1703..1981)
                     /locus_tag="VF958_01210"
     CDS             complement(1703..1981)
                     /locus_tag="VF958_01210"
                     /inference="COORDINATES: protein motif:HMM:NF015582.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome C oxidase subunit IV family protein"
                     /protein_id="HEX9147754.1"
                     /translation="MADHGHPNYLTIFWILLSMTIVEVGYSFMKVPRPVLIGGLVALA
                     GFKALLVALYFMHLKFERKALGVIVGSTLILGVILVSVGIGEHVLPRP"
     gene            complement(2105..2980)
                     /gene="cyoE"
                     /locus_tag="VF958_01215"
     CDS             complement(2105..2980)
                     /gene="cyoE"
                     /locus_tag="VF958_01215"
                     /EC_number="2.5.1.141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011686097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008495 - protoheme IX farnesyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006783 - heme biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme o synthase"
                     /protein_id="HEX9147755.1"
                     /translation="MEVVLEAVPTRVTDYVELTKPRITFLVLVTTAVGYALGAATSFH
                     PGVFIALLAGTALVSGGASALNQWAERDADARMTRTASRPLPTGRLAPADALAFGLVI
                     SAVGLVLLAGAVNWLTALLALGSLSSYVLAYTPLKRVTSLCTLVGAVPGAIPPMMGWA
                     AARGTLDRQAWALFGVLFLWQLPHFLSIAWIYREDYARGGFPMLPVTDPGGASTARQS
                     IAYAAALVPVTLLAGAFAAAGNLYLWGAAGFGVVFVGCTIAFALRRSVRAARLVFLVS
                     VLYLPAVLALMVLDR"
     gene            complement(2971..>3649)
                     /locus_tag="VF958_01220"
     CDS             complement(2971..>3649)
                     /locus_tag="VF958_01220"
                     /inference="COORDINATES: protein motif:HMM:NF014665.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0016653 - oxidoreductase activity, acting
                     on NAD(P)H, heme protein as acceptor [Evidence IEA];
                     GO_process: GO:0006784 - heme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="COX15/CtaA family protein"
                     /protein_id="HEX9147756.1"
                     /translation="PRRWVRRLGWLAVAAVVAQGVLGGLTVLFLLPTAISVAHACLAQ
                     TFFCLAVAIAVVTSPRWERGSAVEPVTRLAALTAAAVFLQLMVGAVMRHTKAGLSIPD
                     FPLSLGRVIPPLDTFPVAIAFAHRAWAVVVTILVAVTLAAAFRSGRSGVSRASLALGA
                     VLVLQIGLGAATVLSRKNVSVTTAHVATGALLLGTAVALAVGSLSRRRLAVVSSQPVE
                     AKVVAWK"
BASE COUNT          738 a         1169 c         1294 g          448 t
ORIGIN      
        1 cgactcgtag taggcgaggt agaacgcctt gacgaccgag gccgggtagt acgggacgtc
       61 cccgcggtac gacccgcccg ccagagccgc tctcgagccg cgctgcgcca ggtccacgac
      121 cgagatcgag agtccctccg cggtcaggcc cctgtcggcg aactcaccga cgacctcggc
      181 tgcgacgcgg gagagaacga cctcgaggtt gctggatcgc tcgatcgcgg cgaccgggcg
      241 gacttccgct ccgcggagag agagcgagag gaggagcagg agaagtgaga gggcggtccg
      301 cctcaggacc gcgctccccg ggagtccggg acgaaccggc cgacggcggc ctcgggcagc
      361 gtagaagact gcagcgggtg caggcccgca gggcctgtcc ccgcgacttt ccccaagcgc
      421 cgccgctttc ggaagatcag gaacgccacg gtgccgacgg ccgtccacgg caccgcgatc
      481 atgagcagaa tcgagtaata gatgccgagg ccgaagccgc gcgtgccacc cggtgtgtcg
      541 gcctccgcgt ccttgcacag ggggcaagcg gagacgagcg ccggaagcag gatcaccgcg
      601 agaacgagaa tcgcggcgac gcgggcagct cgactcatcg cccggatgct aacgaatcgc
      661 cgggcgggcg taaaggtggt agagcatgag gtagacgacg acgcccgtga ccgagacgta
      721 gagccagatc gggaacgtcc acttcgcgat ccgccgatgc cggtcgtcgc gccgcttcaa
      781 acccaggtag agggtcctca gaacgagcgg gagcacgacc atcgcgagaa gagtgtggga
      841 cccgagaatc gcgaagtaca ccgtccgggc cgcgccggtc cccgcgaagc gaactgagcc
      901 cacgcgagcg tggtaggcga cgtaggaaac aaggaagaga gccgacgtcg aggctgccgt
      961 caacatccag cggcggtgtc gttcgatctg tcttcctcgg atggcgcggt aggcgatcag
     1021 gaggaaaacg gcgctcagag cgttgagcgt cgcgttcagg gtgggatgta gaagttccgt
     1081 cccgtttccc ggctcagaac aggtaaatga tcgtgaaaag aatgatccag accaggtcga
     1141 cgaagtgcca gtagaggccg atcgtctcga ccccctggtg gtgggcctgc gtgtactttc
     1201 cgaggaacgc ctttccggtc acgaacgcca tgcagaccac tccgcagaag acgtggaacc
     1261 cgtggaagcc ggtcgtcgtg tagaagacgg atccgaagag cgagacgtgc gggttgaagc
     1321 cggcgtggag caggtggcgg tactcgtaaa cctgtatcga gagaaagatc gcgcctccca
     1381 gcagcgtcat cgagaggaag agtttcagct tgcccaggtc gccgttgcgc acggactcga
     1441 gcgccttcac catcgttacc gacgagcaaa tcaggatgaa ggtgttcagc gcggtcagcc
     1501 agatgttgag ggtgttcccg ggatggggcc acgacgggct cgcaaaccgc aggacgatgt
     1561 aggaaccgat caggccggtg aagaacatca cctcggagca caggaagacc cacatcccca
     1621 tcttgtcgtg cgggattccc gtcgccgacg ggggctcgag ggcgtgatcg tgggcgatgg
     1681 cgtggctgct catggctctt tctcagggtc tcggcagcac gtgctcgccg atcccgaccg
     1741 acaccaggat gacgccgagg atcagggtcg agccgacgat cacgccgagg gccttgcgtt
     1801 cgaacttcag gtgcatgaag tacagggcga cgaggagagc cttgaagccg gcgagcgcga
     1861 cgagcccccc gatcaggacc ggtcgcggca ctttcatgaa cgaatagccc acctcgacga
     1921 tcgtcatcga cagcaggatc cagaagatcg tcaggtagtt gggatgtcca tggtcggcca
     1981 tgctccccct cctacgtttg cgcgcgggaa atgatgaaga gaatcgaccc gatgaagaga
     2041 gctgcggcga ccaggatgag gatcacgagg acgcggcggt tcgaatcgag acgcatgacc
     2101 tctctcaccg atcgagcacc atcagcgcga ggaccgccgg caggtagagc accgagacga
     2161 ggaacacgag gcgcgccgcg cggaccgagc gccggagggc aaaggcgatc gtgcagccga
     2221 cgaagacgac gccgaagccg gccgcgcccc acaggtagag gttcccggcc gccgcgaacg
     2281 cgcctgccag caacgtcacc ggcacgagcg ccgcagcgta cgcgatggac tggcgcgccg
     2341 tcgaagcccc tcccggatcg gtcacgggca gcatcggaaa cccgccccgc gcgtagtcct
     2401 cgcgatagat ccacgcgatc gagaggaagt gcgggagctg ccagagaaag agcaccccga
     2461 agagcgccca tgcctggcgg tccagggtgc cgcgcgcggc cgcccacccc atcatcgggg
     2521 ggatcgctcc cgggacggcc ccgacgagag tgcagaggga cgtcacccgc ttcagcggcg
     2581 tgtacgcgag cacgtaactc gagagcgagc ccagggcgag cagcgccgtc agccagttga
     2641 cagctcccgc gaggaggacg aggccgaccg ccgagatcac gagcccgaag gcgagggcgt
     2701 cggccggcgc gagccggccc gtcggaagcg ggcgcgaagc ggtccgcgtc atccgcgcgt
     2761 cggcgtcgcg ctcggcccac tggttcaggg ccgaggcgcc tccggagacg agggccgtcc
     2821 cggcgaggag cgcgatgaat acgcccggat ggaaactcgt cgcggcgccg agcgcgtacc
     2881 ccacggccgt ggtgacgagc acgaggaacg tgatgcgcgg cttcgtcagc tcgacgtagt
     2941 ccgtgacgcg ggtcgggacc gcctcgagaa ctacttccac gcgaccacct ttgcctcgac
     3001 cggttgcgag gaaacgacgg cgagccgccg gcgcgaaagc gaccccaccg cgagggcgac
     3061 ggccgtcccc aggagcaggg ctcctgtcgc gacgtgcgcc gtcgtcacgg agacgttctt
     3121 tcgcgaaagg accgtcgcgg ccccgaggcc gatctgcagg acgaggacgg cgccgagcgc
     3181 gagcgaagcg cgggaaactc cgctgcgccc cgaccggaac gcggccgcga gagtgaccgc
     3241 gacgaggatc gtcacgacga ccgcccatgc ccggtgcgcg aacgcgatcg cgacgggaaa
     3301 ggtgtcgagc ggagggatca cccggccgag cgaaagcgga aaatccggaa tggacaggcc
     3361 ggctttggtg tgccgcatga cggcgccgac catgagttgc aggaacacgg cggccgcggt
     3421 cagcgcggcg agccgagtga cgggctccac cgccgatccg cgttcccagc gcggagaagt
     3481 cacgaccgcg atcgcgaccg ccaggcagaa gaacgtctgc gcgagacagg cgtgcgcaac
     3541 ggagatggcc gtcggcagca ggaacaggac cgtcaggccc ccgagcacgc cctgggcgac
     3601 gaccgccgcg accgcgagcc atcccagccg gcggacccag cggcgcggc
//
LOCUS       DASWAV010000054         4886 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U2665
            k141_162641, whole genome shotgun sequence.
ACCESSION   DASWAV010000054 DASWAV010000000
VERSION     DASWAV010000054.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960316
            Sequence Read Archive: SRR10260007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 4886)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4886)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:47:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,929
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4886
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_162641"
                     /isolate="SMAG_U2665"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.36444 N 132.31889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..693)
                     /gene="cdaA"
                     /locus_tag="VF958_01225"
     CDS             complement(<1..693)
                     /gene="cdaA"
                     /locus_tag="VF958_01225"
                     /EC_number="2.7.7.85"
                     /inference="COORDINATES: protein motif:HMM:TIGR00159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004016 - adenylate cyclase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diadenylate cyclase CdaA"
                     /protein_id="HEX9147757.1"
                     /translation="MIDFLTARFGSPGSLSFTWRDAVDILIVAFVVYFLLRLIRGTRA
                     VQMVFGLIAILVVYWISVLLKLVALRTVLTALIFYLPFAIIVLFASELRRALAAFGRT
                     PFFALFSSYRAEETVSDLVLAATSLSTRRVGALIVLERREGLKTYIENGVPLDAAISY
                     DLLVTLFAPGTPLHDGAVIVSRERVAAASCFLPLSLKEGLSKRFGTRHRAAMGVTEET
                     DALAIVVSEERGT"
     gene            complement(690..1838)
                     /gene="folP"
                     /locus_tag="VF958_01230"
     CDS             complement(690..1838)
                     /gene="folP"
                     /locus_tag="VF958_01230"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR01496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004156 - dihydropteroate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HEX9147758.1"
                     /translation="MAVSADLVFPAATPELLREIASWDGVRLATFAASDFPVDVVRLE
                     RSASPPIVLSRGKRAESLKALPASLRERALEAFGRAEARRPFLSVARGRKIDLSRPPI
                     VMGVVNVTPDSFSDGGVYLDPARAVARALEMFEEGAAIVDVGGESTRPADYGEAATVP
                     AEEEISRILPVIEGIRRATDRPISVDTRKAAVARRALAAGADLVNDVSALRYDPEMVA
                     AVAGGDAGLLLMHMKGNDPRTMQADTSYEHPVADVAAELAAAASKALDAGIGAGRIAV
                     DPGLGFGKTPEGNLLLLRHLSAFASLGFAVAVGASRKGFVRRFSGVTEDSPAADRLPG
                     SLAALAAARAGHASIVRVHDVAESVRFLRMLDAIEAPAVRQPAEAAAR"
     gene            complement(1849..2340)
                     /locus_tag="VF958_01235"
     CDS             complement(1849..2340)
                     /locus_tag="VF958_01235"
                     /inference="COORDINATES: protein motif:HMM:NF017015.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HEX9147759.1"
                     /translation="MTIADVFLREFDLEMETTRHVLERVPSGRLDWRPHPKSMTMGEL
                     AGHVAELATWGLRMDEPSFEEGTRPRVPFESVGELLARFDENVAASRAAISRKTDDDL
                     RERLTVTRGSATYKMSKASVLRRVFLNHLIHHRGQLSVYLRLNDVPLPPIYGPSADEA
                     PTG"
     gene            complement(2394..4307)
                     /gene="ftsH"
                     /locus_tag="VF958_01240"
     CDS             complement(2394..4307)
                     /gene="ftsH"
                     /locus_tag="VF958_01240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014784642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HEX9147760.1"
                     /translation="MNATIKNLLLWMVILVLIILAWNVFKTGATAVEEISFSQFLDRV
                     AQGHVQKVNIRGEEIRGTYAASAGRREPLFKTYAANYPDLVKDLRARGVEIEIQPLKD
                     SSFVSALVMYGPLVLLVAIWFVIMRQMQAGGNKALSFGKSRAKLLTPTQKKVTFKDVA
                     GVEEAKVELQEIIEFLKDPQRFQKLGGKIPKGVLLMGPPGTGKTLLARSVAGEANVPF
                     FTISGSDFVEMFVGVGASRVRDLFEQGKKNAPCIVFIDEIDAVGRHRGAGLGGGHDER
                     EQTLNALLVEMDGFDTNEGVILVAATNRPDVLDPALLRPGRFDRRVVVDRPDLRGRDQ
                     ILKVHTRQTPLSENVDLSIIARGTPGFSGADLANLVNEAALNAARFNKKKVEMIDFEY
                     AKDKVMMGPERKSMIMTEEEKKNTAYHEGGHALVAAMQPDADPIHKVTIIPRGRALGV
                     TMQLPMEDKYSHTKEYLEATITVMMGGRIAEETFLGHMTTGAGNDIEKATDVARKMVC
                     EWGMSDMGPLTFGKPDGEIFLGRDFGRTQDYSESTANQIDAEVKKIVTAAYDRAEKIV
                     TGNKNVMHRLARTLLEKEVLDGEEVLQIIAEETGTDISKLRKSGPPKPPIVTPPGAPE
                     VADDVGPLVPEPA"
     gene            complement(4355..>4886)
                     /locus_tag="VF958_01245"
     CDS             complement(4355..>4886)
                     /locus_tag="VF958_01245"
                     /inference="COORDINATES: protein motif:HMM:NF013348.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX9147761.1"
                     /translation="REIRADGVVRPLLSVPRRQILDFLNARGIAWRRDRTNGDLSLAR
                     NRLRRELVFQGEDTEALAHRVEVLARRREELDVAFERNVRPALAFGRNVVIAGAPLLE
                     SSPPELARKAILECAMPFARPGRPPFTGREREQIIALLASGRDFRFEAGRRIRFRRRG
                     DRFSVRLAVAEGAKRA"
BASE COUNT          786 a         1643 c         1672 g          785 t
ORIGIN      
        1 cgtgccgcgc tcctcggaga cgacgatcgc gagcgcgtcg gtttcctcgg tcacgcccat
       61 ggcggcgcgg tggcgcgtcc cgaatcgctt cgacaggcct tccttgagcg agagcggcag
      121 gaagcacgac gccgccgcga cgcgttcgcg ggacacgatg accgcgccat cgtggagggg
      181 agtccccggg gcgaagagcg tgacgagcag gtcatacgag atcgcggcgt cgagggggac
      241 gccgttctcg atgtacgtct tcagcccctc gcgccgctcg agaacgatca gcgcgccgac
      301 acgccgcgtc gacagcgacg tcgccgcgag cacgaggtcg gagaccgtct cctccgcccg
      361 atagctcgag aacagagcga agaacggcgt gcggccgaac gccgccagcg cccgccgcag
      421 ctccgacgcg aagaggacga tgatcgcgaa gggcaggtag aagatgagcg cggtgagcac
      481 ggtgcgcagc gcgacgagct tcaggaggac cgagatccag tagacgacga ggatcgcgat
      541 cagcccgaag accatctgga cggcgcgcgt cccgcggatc aggcgcagca ggaagtagac
      601 gacgaaggcg acgatgagaa tgtcgacggc gtcgcgccag gtgaaagaca gacttcccgg
      661 ggacccgaaa cgcgccgtca ggaagtcgat caccgcgcgg ccgcctcagc gggctgccgg
      721 acggccgggg cctcgatcgc gtcgagcatc ctcaaaaacc tcaccgactc ggccacgtca
      781 tggacgcgca cgatggacgc gtgcccggca cgcgcggcgg cgagcgccgc gagcgacccg
      841 ggcagccgat cggcggccgg ggagtcctcg gtgacgccgg agaagcggcg gacgaagccc
      901 ttgcgcgagg cgccgactgc gaccgcgaag cccagcgagg cgaaagccga gaggtgccgc
      961 aggaggagca ggttcccctc gggggtcttc ccgaagccga ggccgggatc gacggcgatc
     1021 cgcccggccc cgatccccgc gtcgagcgcc ttcgacgccg ctgcggcgag ctccgcggcg
     1081 acgtcggcga ccgggtgctc gtaggacgtg tccgcctgca tcgtgcgcgg gtcgttcccc
     1141 ttcatgtgca tgaggagcag gccggcgtcc ccgccggcga cggcggcgac catctccgga
     1201 tcgtaacgga gcgcggaaac gtcgttgacg aggtccgcgc ccgccgcgag cgcccgccgc
     1261 gcgaccgcag ccttgcgcgt gtccacggag atgggacggt cggtcgcgcg gcggatccct
     1321 tcgatgaccg ggaggatccg cgagatctct tcctccgcgg gaaccgtcgc ggcctcgccg
     1381 tagtccgccg ggcgagtgga ttcaccgccg acgtcgacga tcgcggcgcc ctcctcgaac
     1441 atctcgaggg cgcgagcgac cgcgcgggcc gggtcgaggt agacgccgcc gtcggagaac
     1501 gagtcgggcg tgacgttcac gacgcccatg acgatcggcg ggcgcgacag gtcgatcttc
     1561 ctcccgcgag cgactgaaag aaacggccgc cgcgcttccg cgcgcccgaa tgcctccagg
     1621 gcgcgctcac ggaggctcgc gggaagggcc ttcaggctct ccgcgcgctt gcctcgggac
     1681 aggacgatcg gcggagaagc ggagcgctcg agccggacga cgtcgacggg aaagtcggac
     1741 gcggcgaacg tcgcaagccg caccccgtcc caggacgcga tctcgcgcag gagctcgggg
     1801 gtcgccgccg ggaagacgag gtcggctgag accgccacgg gcgtcagttt agcctgtggg
     1861 cgcctcgtcg gccgatgggc cgtagatcgg cggaagcgga acgtcgttca ggcggaggta
     1921 taccgagagc tgcccgcggt ggtggatgag gtggttcagg aaaacgcgcc ggagaacgct
     1981 cgccttggac atcttgtagg tcgcgctgcc gcgggtgacc gtcaatcgtt cccgcaggtc
     2041 gtcgtcggtc ttgcgggaga tcgccgcgcg gctcgccgcg acgttctcgt cgaatcgggc
     2101 gaggagctcc ccgaccgact cgaacgggac tcgcggccgc gtcccctcct cgaacgaagg
     2161 ctcgtccatc cgcaggcccc aggtcgcgag ctcggcgacg tgcccggcga gctctcccat
     2221 cgtcatggat ttcgggtggg gccgccagtc gagccgcccg gacgggacgc gctccagcac
     2281 atgccgcgtc gtctccatct cgaggtcgaa ctcgcggaga aaaacgtcgg cgatggtcac
     2341 tggcgcattc tccttctccc cgcttgaggg aggagggtcg gggtgagcgg ccgctacgcc
     2401 ggctcgggga ccagcggtcc gacgtcgtcc gccacttccg gtgcgccggg cggcgtcacg
     2461 atcggcggct tgggaggtcc ggacttgcgc agcttcgaga tgtccgttcc cgtctcctcc
     2521 gcgatgatct ggagaacctc ttcgccgtcg aggacctcct tctcgaggag ggtccgggcg
     2581 aggcggtgca tcacgttctt gttgccggta acgatcttct ccgcccggtc gtaggccgcg
     2641 gtcacgatct tcttgacctc ggcgtcgatc tggttcgcgg tcgactcgga gtagtcctgc
     2701 gtgcggccga agtcgcggcc gaggaagatc tctccgtccg gtttgccgaa cgtgagcggg
     2761 cccatgtcgc tcatgcccca ctcgcagacc atctttcggg cgacgtcggt tgctttctcg
     2821 atgtcgttgc cggcccccgt cgtcatgtgg ccgaggaagg tttcctcggc gatgcgaccg
     2881 cccatcatga cggtgatcgt cgcctcgagg tactccttcg tgtgcgagta cttgtcttcc
     2941 atcgggagct gcatcgtcac gccgagcgcg cgtcctcgcg gaatgatcgt caccttgtgg
     3001 atcgggtccg catccggctg catcgcggcg acgagcgcgt gtccgccctc gtgatacgcg
     3061 gtgttctttt tctcctcttc ggtcatgatc atcgacttgc gctccgggcc catcatgacc
     3121 ttgtccttcg cgtactcgaa gtcgatcatc tcgaccttct tcttgttgaa tcgggccgcg
     3181 ttcagagccg cctcgttgac gaggttggcg aggtcggcgc cggagaaacc gggcgttccc
     3241 cgggcaatga tcgagagatc gacgttctcc gagagcggcg tctggcgcgt gtggactttc
     3301 aggatctgat cgcggccgcg caggtcgggc cggtcgacca cgacgcgccg gtcgaagcgg
     3361 ccgggacgaa gcagcgccgg gtcgagcacg tccggtcggt tcgtggccgc cacgaggatg
     3421 acgccctcgt tcgtgtcgaa accgtccatc tcgacgagga gagcgttcag cgtctgctcc
     3481 ctctcgtcgt gcccaccgcc gaggccggcg ccgcgatggc ggccgacggc gtcgatctca
     3541 tcgatgaaga cgatgcacgg ggcgttcttc ttgccctgct cgaagaggtc gcgcacgcgg
     3601 gacgcgccga cgcccacgaa catctcgacg aagtcggagc cggagatcgt gaagaaggga
     3661 acgttcgcct caccggcgac ggagcgcgcc aggagcgtct tgcccgtacc cggaggaccc
     3721 atcaggagca cgcccttcgg gatctttcca ccgagcttct ggaacctctg cgggtccttc
     3781 aggaactcga tgatctcctg cagctcgacc ttggcttcct cgactcccgc cacgtccttg
     3841 aaagtgacct ttttctgcgt gggggtgagc agcttcgcgc gcgacttgcc gaaggagagc
     3901 gccttgtttc cgccggcctg catctgccgc atgatcacga accagatcgc gacgagcagg
     3961 accagcgggc cgtacatcac gagagccgaa acgaacgagg agtccttgag cggctggatc
     4021 tcgatctcca cgccccgggc gcgcaggtcc ttgacgaggt cgggataatt cgcggcgtac
     4081 gtcttgaaga gcggctcgcg gcgtccggcg gacgctgcgt acgttccccg gatctcctct
     4141 ccgcggatat tgactttctg gacgtgtccc tgggccactc gatcgaggaa ctgcgagaag
     4201 ctgatctcct cgacggccgt cgctccagtc ttgaaaacgt tccacgcgag gatgatcaac
     4261 accaggatga ccatccacag cagcaagttc ttgatcgtgg cgttcaaacc agtactcctt
     4321 gtggaccctc aggataacgg agtcttctcg acctttatgc cctttttgcg ccctccgcca
     4381 ccgcgagccg gaccgagaat cggtcgcccc gccgtcggaa ccggatcctc cgacccgctt
     4441 cgaagcgaaa atcgcgcccg ctcgccagaa gcgcgatgat ctgctcgcgt tcgcgccccg
     4501 tgaaaggggg ccttcccggc ctcgcgaacg gcatcgcgca ctccaggatc gcttttctcg
     4561 ccagctcagg cggcgagctc tcgagcagcg gcgccccggc gatcaccacg ttcctgccaa
     4621 acgcaagcgc cgggcgaacg ttcctctcga acgcgacgtc gagctcttcg cggcggcgcg
     4681 cgaggacctc gacgcggtga gcgagggcct cggtgtcctc tccctgaaat acgagctcgc
     4741 ggcgcagccg gttgcgcgcg agcgacagat cgccgttcgt ccgatccctc cgccaggcga
     4801 tcccgcgggc gttcaggaaa tccaggatct ggcggcgggg gacggaaagc agcggccgca
     4861 cgacgccgtc ggcgcgaatc tcgcgg
//
LOCUS       DASWAV010000055         1578 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U2665
            k141_522897, whole genome shotgun sequence.
ACCESSION   DASWAV010000055 DASWAV010000000
VERSION     DASWAV010000055.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960316
            Sequence Read Archive: SRR10260007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 1578)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1578)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:47:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,929
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1578
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_522897"
                     /isolate="SMAG_U2665"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.36444 N 132.31889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1578)
                     /locus_tag="VF958_01250"
     CDS             complement(<1..>1578)
                     /locus_tag="VF958_01250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011523715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF885 family protein"
                     /protein_id="HEX9147762.1"
                     /translation="MLNGSSPAASAATPAGAPATDLEARRKQMNDLLAEHWEYSLSHS
                     PEFASILGDKRWNDKLSDFSQEAIEKDLAKTKEFLGRFEAIDPTGFPEQEALTRTLLV
                     RSFREQLEDARFKNWEIPVTQIYGIHIDAPQLVSLLSFESGKDYDDYVARLKALPLLM
                     DQTVIQMRKGMAEGLIPPKFLLEKVVAQAQGIAEKKPEESPFAMPLSKMPASFSEEEK
                     ARIRSAVVGAIRDSVAPAYVKFTKFVKEDYAPRGRKDPGMWSLPDGQARYSTVVKRQT
                     TTDLTPEQIHQIGVEQVALIETDMATVAQRLGFADWKALAASIEKDPKRHFHSREEIL
                     AAYRKFIDRMKPQLPKLFGRLPKADLVVMQVEEFREKEASGAQYNPGTPDGSRPGHVM
                     VNTSDPETRKTISTESTAYHEGVPGHHLQIAIAQELEKMPPQRQHAFYTAYVEGWALY
                     SERLGKEIGFYEDPYSDFGRLQDEMLRAIRLVVDTGFHAQKWTRQQVVDYFHAHSAID
                     EVDVQSETDRYISIPGQA"
BASE COUNT          238 a          522 c          502 g          316 t
ORIGIN      
        1 gcctggccgg ggatcgagat gtatcggtcg gtctcgctct gaacgtccac ctcgtcgatc
       61 gccgagtgcg cgtggaagta gtcgacgacc tgctgccgcg tccatttctg ggcgtgaaaa
      121 ccggtgtcga cgacgagccg gatcgcgcgc agcatctcgt cctgaaggcg gccgaagtcg
      181 ctatacgggt cctcgtagaa cccgatctcc ttgccgagcc gctcggaata cagggcccac
      241 ccttcgacgt acgccgtgta gaacgcgtgc tggcgctgcg gcggcatctt ctcgagctcc
      301 tgcgcgatcg cgatctggag gtgatgcccc ggcacgccct cgtggtacgc cgtcgactcc
      361 gtcgagatcg tcttgcgcgt ctcggggtcg ctcgtgttga ccatcacgtg cccgggccgc
      421 gagccgtccg gcgttccggg gttgtattgc gcgcccgacg cctccttttc acgaaattcc
      481 tcgacctgca tcacgacgag atccgccttc ggcagccgcc cgaagagctt gggcagctgc
      541 ggcttcatcc ggtcgatgaa cttgcggtac gccgcgagga tctcctcgcg cgagtggaaa
      601 tggcgtttcg ggtctttctc gatcgacgcg gcgagcgcct tccagtcggc aaacccgagc
      661 cgttgcgcca cggtcgccat atccgtctcg atcaacgcga cctgctcgac gccgatctgg
      721 tggatctgtt cgggagtgag gtccgtcgtc gtctgccgct tcaccaccgt cgagtaccgc
      781 gcctgaccgt ccggcaggga ccacatcccc gggtctttcc tccctcgcgg tgcgtaatcc
      841 tccttgacga acttcgtgaa cttcacgtac gccggggcga ccgagtcgcg gatcgcgccc
      901 acgaccgccg agcggattcg cgccttttcc tcctccgaga acgaagccgg catcttcgac
      961 agcggcatcg caaacggcga ctcctccggt ttcttctccg cgatgccctg cgcctgcgca
     1021 acgactttct cgagcaggaa cttcggcggg atgagcccct cggccatgcc cttgcgcatc
     1081 tggatcaccg tctggtccat gagcagcggc agcgctttca ggcgggcgac gtagtcgtcg
     1141 tagtccttcc ccgactcgaa ggagaggagc gagacgagct gcggagcgtc gatgtggatg
     1201 ccgtagatct gcgtgacggg aatctcccag ttcttgaacc gtgcgtcttc gagctgctcg
     1261 cggaaactgc gcacgaggag cgtccgtgtg agcgcctcct gctcgggaaa gccggtgggg
     1321 tcgatcgcct cgaaccggcc gaggaactcc ttcgtcttcg cgaggtcctt ctcgatcgct
     1381 tcctgcgaga agtcggacag cttgtcgttc caccgcttgt cgccgaggat ggacgcgaat
     1441 tcggggctgt gggagagcga gtactcccag tgctcggcga gcaggtcgtt catctgcttg
     1501 cgccgcgcct cgagatccgt cgccggagct cccgcaggag tggctgcgga tgcggcggga
     1561 gacgatccgt tcagcatg
//
LOCUS       DASWAV010000056         2244 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U2665
            k141_642941, whole genome shotgun sequence.
ACCESSION   DASWAV010000056 DASWAV010000000
VERSION     DASWAV010000056.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960316
            Sequence Read Archive: SRR10260007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2244)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2244)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:47:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,929
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2244
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_642941"
                     /isolate="SMAG_U2665"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.36444 N 132.31889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            245..547
                     /locus_tag="VF958_01255"
     CDS             245..547
                     /locus_tag="VF958_01255"
                     /inference="COORDINATES: protein motif:HMM:NF039822.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5666 domain-containing protein"
                     /protein_id="HEX9147763.1"
                     /translation="MTRTLKTSLAAIAAVVLVLALGGKAAAAEKTLTASGTVSRLDAA
                     QRTVVVAVTEGPETTFAWTADTKINGTLAPGAKVTIRYTTLPDGQNLAHQISVARS"
     gene            607..879
                     /locus_tag="VF958_01260"
     CDS             607..879
                     /locus_tag="VF958_01260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007906263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin family protein"
                     /protein_id="HEX9147764.1"
                     /translation="MGMYIIAEPCIGTKDTACVDVCPVDCIHPRKDEKEFETAEMLYI
                     DPDVCIQCGNCEPACPVTAIFTEETIPDKWKHFQQINADWYKNRQG"
     gene            complement(897..1655)
                     /locus_tag="VF958_01265"
     CDS             complement(897..1655)
                     /locus_tag="VF958_01265"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011523589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HEX9147765.1"
                     /translation="MSKIMVLIVATLAAAASSLAAPAGKMATYPSGSETVSGYLALPE
                     GAGRKPALVVIQEWWGLNDFVKGKADEFAKKGYVAFAPDLYRGKVATDPDTAHQLMRG
                     MPEDRAIRDLKAAFEYLRSRDDVDASRIGSVGWCMGGGYSLALALEEPRLAATVMYYG
                     RLVTDDAKIKSLSSPLLGNFGGQDQGIPPESVLEFARKAKAAGKSVDFKVYPEAGHAF
                     ASSTDPKVFRTADAKDADARADAFLARVLKARKS"
     gene            1690..2202
                     /locus_tag="VF958_01270"
     CDS             1690..2202
                     /locus_tag="VF958_01270"
                     /inference="COORDINATES: protein motif:HMM:NF021759.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprT-like domain-containing protein"
                     /protein_id="HEX9147766.1"
                     /translation="MSSLFPVEPEDESARLQFLYDRLAARFGFSAAEVRLSDRKLTGG
                     EIVYGRPHRITISSHLSFVEREDTLRHEAAHAWAFELEGARAGHGPLFRRLSRHIGAS
                     PRKAPLTPALAEHRRRNEVLYRCDGCRRVFRRYRAIRGARYCVRCDRAGRPSRLKRLA
                     RETGNGKRER"
BASE COUNT          381 a          748 c          717 g          398 t
ORIGIN      
        1 ctcgagcttc ttttccagct cgtcgatcct cacggaaagc gcgtcggcca gggcctgggg
       61 gctcatcttc gacgcgttgt ctctcaggaa tcgaagctcg gcttctgtcc agcgttttgc
      121 cattctgtta cctccggggg ccgcgagaaa aacggcgtat tgtagggacg gactctccgc
      181 gtgtcaacgg cgaacgcgcc ggaatggctg aactcgaagg aacgttctct tcggggaggt
      241 gggtatgacg agaactttga agacttcgct cgcggccatc gccgctgtgg tgctcgttct
      301 cgcgcttgga ggaaaagctg ccgccgccga gaagactctg acggcgtccg ggacggtttc
      361 ccggctcgat gctgcccagc gcacagtcgt cgtggccgtg acggaggggc ccgagacgac
      421 gttcgcctgg acggccgaca cgaagatcaa cggcactctc gcccccgggg cgaaggtcac
      481 gatccgctac acgaccctcc ccgacggcca gaacctggct caccagatca gcgtggcgcg
      541 aagctagctc tccctttccg gtagaatcgc cgctcggcga aacccgccgc ggaggaatac
      601 gactcaatgg gcatgtacat aattgcggag ccctgcatcg gcacgaagga cacggcctgt
      661 gtcgacgtgt gtccggtcga ctgcatccat ccgcgcaagg acgagaagga attcgagacg
      721 gccgagatgc tctacattga tccggacgtc tgcattcagt gcggcaactg cgagcctgcg
      781 tgccccgtga ccgcgatctt cacggaagaa actatccccg acaagtggaa gcacttccag
      841 cagatcaacg cggactggta caagaaccgg cagggttagc cgccggtcct cccgatctaa
      901 ctttttcgcg ccttcaagac tctcgcgagg aacgcgtccg cacgcgcgtc ggcgtccttc
      961 gcgtcggccg tccgaaagac cttgggatcg gtcgacgacg cgaatgcgtg gccagcttcc
     1021 ggatagacct tgaagtccac gctctttccc gccgccttcg ccttgcgtgc gaactcgagg
     1081 acgctctcgg gcggaatacc ctggtcctgg ccgccgaagt tgccgagcag aggcgacgac
     1141 aggctcttga tcttcgcgtc gtcggtgacg agccgcccgt agtacatcac ggtggccgcg
     1201 agcctcggct cctcgagcgc cagcgcgagc gagtagccgc cgcccatgca ccatccgacc
     1261 gatccgatgc gggacgcgtc cacgtcgtcg cgcgaacgga ggtattcgaa cgcggccttc
     1321 agatcccgga tcgcgcggtc ctcgggcatg ccgcgcatga gctgatgcgc ggtgtcggga
     1381 tccgtcgcga ccttgccgcg atagaggtcg ggcgcgaatg cgacgtagcc cttcttcgcg
     1441 aactcgtccg ccttgccttt gacgaagtca ttcaatcccc accactcctg gatcacgacg
     1501 agcgcgggtt tgcgccccgc cccttcgggg agcgcgaggt accccgaaac cgtctcggag
     1561 ccgctcggat acgtcgccat ctttccggcc ggtgccgcga gggagctcgc cgcggccgcg
     1621 agagtcgcca cgatcaggac catgattttc gacattcttc gatcacctcc atcgggttaa
     1681 tcttatggcg tgagctctct cttcccggtt gagccggagg acgagagcgc ccgtcttcag
     1741 tttctctacg accgcctggc ggcccggttc ggcttctcgg ccgcggaggt gcgtctctcc
     1801 gaccgcaagc tgaccggcgg cgagatcgtc tacggaaggc cgcaccggat cacgatctcg
     1861 tcgcacctct cgtttgtcga gcgcgaggac acgctgcgcc acgaagccgc gcacgcgtgg
     1921 gccttcgagc tcgagggggc gcgcgccggc cacggcccgc tcttccgccg cctctcgcgt
     1981 cacatcgggg ccagtccgcg taaggcgcct ctgacgccgg cgctcgccga gcaccggcgt
     2041 cggaacgaag tcctctaccg ctgcgacggc tgccgccgcg tgttccgccg ctatcgcgcg
     2101 atccgcggag cacgatattg cgtgcggtgc gaccgggcgg ggagaccgag ccggctgaag
     2161 cgcctcgcgc gggaaacggg aaacgggaaa cgggaaaggt gagaccgcgg aaggcttctt
     2221 ttccgaccgg gtatcggatc gggc
//
LOCUS       DASWAV010000057         3401 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U2665
            k141_83358, whole genome shotgun sequence.
ACCESSION   DASWAV010000057 DASWAV010000000
VERSION     DASWAV010000057.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960316
            Sequence Read Archive: SRR10260007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 3401)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3401)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:47:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,929
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3401
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_83358"
                     /isolate="SMAG_U2665"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.36444 N 132.31889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..208)
                     /gene="deoA"
                     /locus_tag="VF958_01275"
     CDS             complement(<1..208)
                     /gene="deoA"
                     /locus_tag="VF958_01275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019006503.1"
                     /note="Catalyzes the reversible phosphorolysis of
                     thymidine, deoxyuridine and their analogues to their
                     respective bases and 2-deoxyribose 1-phosphate; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine-nucleoside phosphorylase"
                     /protein_id="HEX9147767.1"
                     /translation="MRTVYEILRAKRSGEELPKEEITYLVEGVVSGEVPEYQASAFLM
                     AAVILGLSTAETAALTEAMLDSGEA"
     gene            complement(205..1641)
                     /locus_tag="VF958_01280"
     CDS             complement(205..1641)
                     /locus_tag="VF958_01280"
                     /inference="COORDINATES: protein motif:HMM:NF014081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HEX9147768.1"
                     /translation="MRRASLLVAFVLLLGAATPARREPADLLITGGTVATVDGNFSVY
                     SPGAVAIRGGEIVAVGPAADIGARYAARERFDASGKIVLPGLVNTHTHAAMTLLRGLA
                     DDLPLDRWLTEHIFPAEKMNVSPGFVYDGTLLAALEMIEGGTTTFADMYYFESDVARA
                     VDKAGLRAVLGETFIDFPVPDHKDLAETLAFMDSFVKRWKGHPRIVPAPAPHAIYTNA
                     KETLLAARDFAHREKLPVLIHVAETRKEYTEAAERWRKTPFAYLASIGFFDPPPGGAR
                     LPIVVAHAVWMDPSDRALVAQYGIGLSHNPESNMKLSSGIADVTAWEKDGLLWALGTD
                     GPAGSNNDLSMFEAMDFAGKLAKVSTGDPTALPAREIVAAATREGARVLGFGDKTGSL
                     ERGKRADVIVLDARTAHEQPFEDVYSTLVYSAKASDVTDVWVDGKRLLANRQCRTLDR
                     RAIIETAAKWRRKVHDGLAAAAKEKPTP"
     gene            complement(1638..2030)
                     /locus_tag="VF958_01285"
     CDS             complement(1638..2030)
                     /locus_tag="VF958_01285"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015869046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004126 - cytidine deaminase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0009972 - cytidine deamination [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HEX9147769.1"
                     /translation="MIDPEREERLVAEAFRSRDNSVSHRSGFAVGAAIEAEDGRIFGG
                     CNVENATYGLTVCAERVAIWKALSEGVRRFRAVAVVTGADTPTPPCGACRQILWEFAG
                     DVPVVSVTTGGRRKTYRMSELLPEPFDL"
     gene            complement(2027..2875)
                     /locus_tag="VF958_01290"
     CDS             complement(2027..2875)
                     /locus_tag="VF958_01290"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009225818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HEX9147770.1"
                     /translation="MTETGLLGRLDTSAGFLRGKRDLAPAVGLVLGSGLGAFAERLKN
                     RVAIPYEAIPHFPVPSGVVGHAGELVLGDVDRTPVVVLSGRVHFYEGRPMSDVVYPAR
                     VLARLGVGTVILTNAAGGVRRTFRPGDLMLMTDHINAFGTNPLIGPNEESLGARFPDM
                     SRVYDPVLRKLAKDTARSLRIPLREGVYLGNSGPSYETPAEIRAYRVLGADAVGMSTV
                     PEAVALRHAGVRVLGISTITNMAAGILPKPLDHSEVLATTKKVGDRFVRLLTALVPKI
                     GRAARK"
     gene            complement(2872..>3401)
                     /locus_tag="VF958_01295"
     CDS             complement(2872..>3401)
                     /locus_tag="VF958_01295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014066102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="nucleoside transporter C-terminal
                     domain-containing protein"
                     /protein_id="HEX9147771.1"
                     /translation="VEKTHTNLIDAAASGASQGMTLVINIVAMLIAFIALIALLNGFL
                     GWAGGLFGHPEFQLQQVFAWVLWPLAVVMGVPPRDAGAVADLIGNKTVINEFFAYAKM
                     STVQGGMSEKARLIASFALCGFANFSSIGIQIGGIGGLAPTRKGDIARLGFRALLGGS
                     LASFMTATIAGLLAG"
BASE COUNT          564 a         1160 c         1154 g          523 t
ORIGIN      
        1 aggcctcgcc cgagtcgagc atcgcctcgg tcagcgccgc cgtctccgcg gtcgaaaggc
       61 cgaggatcac ggctgccatg aggaacgccg aggcctggta ctccggcact tctcccgaca
      121 ccacgccctc gacgaggtac gtgatctcct ccttcggaag ctcctcgccc gaccgctttg
      181 cgcgcaggat ctcgtagacc gtcctcacgg cgtcggcttc tccttcgcgg cagcagcgag
      241 accgtcgtgc accttgcggc gccatttcgc cgctgtttcg atgattgccc ggcgatcgag
      301 cgtccggcac tggcggttgg cgagaaggcg ctttccgtcc acccacacgt cggtgacgtc
      361 ggacgcctta gcggaataga cgagcgtcga ataaacgtcc tcgaacggct gctcgtgggc
      421 cgtgcgcgcg tcgagcacga tcacgtccgc gcgctttccc cgctcgagag accccgtctt
      481 gtcgccgaac cccaggacgc gggcgccctc gcgcgtggct gcagcgacga tttcccgcgc
      541 ggggagcgcg gtcgggtcgc ctgtcgacac cttggcgagc ttcccggcga aatccatcgc
      601 ctcgaacatc gacaggtcgt tgttcgagcc ggcgggccca tcggtcccga gggcccagag
      661 gagcccgtcc ttctcccacg cggtcacgtc ggcgatcccg gacgacagtt tcatgttgga
      721 ttccgggttg tgcgagagac cgatcccgta ctgagcgacg agcgcccggt ccgacgggtc
      781 catccagacc gcgtgcgcca caacgatcgg cagcctcgcg ccccccggcg gcggatcgaa
      841 gaagccgatc gaggcgagat aggcgaaggg cgtctttctc cacctctccg ccgcctccgt
      901 gtactccttg cgcgtctcgg cgacgtggat gagcacgggg agcttctcgc ggtgggcgaa
      961 atcgcgcgcg gccaggagtg tctccttcgc gttcgtgtag atcgcgtggg gcgcgggggc
     1021 cgggacgatc cgggggtgtc ccttccaccg tttcacgaac gagtccatga aggcgagcgt
     1081 ctcggccagg tccttgtgat ccggcacggg gaagtcgatg aacgtttcgc cgagaacggc
     1141 gcgcagcccg gccttatcga cggcgcgtgc gacgtcggat tcgaagtagt acatgtcggc
     1201 gaaagtagtc gtccctccct cgatcatctc gagcgccgcc agcagcgtcc cgtcgtacac
     1261 gaaccccggc gagacgttca tcttctcggc ggggaagatg tgctccgtca gccagcggtc
     1321 gagcggaagg tcgtcggcga ggccgcgaag gagcgtcatc gccgcatgag tgtgcgtgtt
     1381 gacgaggccc gggagcacga tcttgcccga ggcgtcgaac cgctcgcggg ccgcgtacct
     1441 cgcgccgatg tccgcggccg ggcccaccgc gacgatttct ccgccgcgga tcgcgaccgc
     1501 cccgggagag tagaccgaga agttgccgtc caccgtcgcg accgtgccgc cggtgatcag
     1561 gagatctgct ggctcccgcc gggcgggcgt cgccgccccg agaaggagaa cgaaggcgac
     1621 gagaagagag gcgcgtctca aaggtcgaac ggctccggaa gaagctcgga catgcggtac
     1681 gtcttgcgcc tcccgccggt ggtcaccgag accaccggca cgtctccggc gaactcccac
     1741 aggatctgcc ggcaagcgcc gcacggcggc gtcggagtgt ccgcgccggt gacgacggcc
     1801 acggcgcgga accgccggac cccttccgac agggccttcc agatcgcgac gcgttcggcg
     1861 cacacagtca ggccgtaggt cgcgttctcg acgttgcagc caccgaagat ccggccatcc
     1921 tcggcctcga tcgcggcgcc gaccgcgaag ccgctgcggt gagatactga gttgtcgcgg
     1981 ctgcgaaacg cctcggcgac gagccgctct tcccgctccg gatcgatcac ttgcgggcgg
     2041 cgcgcccgat cttcggaacg agcgcggtaa gcagccgaac gaaacggtcc cccaccttct
     2101 tcgtcgtggc gagaacctcc gagtggtcga gaggtttcgg caagattccc gcagccatgt
     2161 tcgtgatggt cgagatgccg aggacgcgca ccccggcgtg gcgcagcgcg accgcctccg
     2221 ggacggtcga catcccgacc gcgtcggcgc cgaggacgcg gtacgcgcga atctccgcgg
     2281 gcgtctcgta cgacgggccg gagttgccga ggtagacgcc ttcgcgaagc gggatccgga
     2341 gcgaacgcgc cgtgtccttc gcgagcttcc ggagaaccgg gtcgtagacc cggctcatgt
     2401 cgggaaaacg cgcaccgaga gactcctcgt tcggcccgat gagcgggttg gtcccgaagg
     2461 cgttgatgtg gtcggtcatc agcatgaggt cgcccggtct gaaggtgcgc cggacgcctc
     2521 cggccgcgtt cgtcaggata accgtgccca cgccgaggcg cgcgaggacg cgcgcgggat
     2581 acacgacgtc ggacatcggc cgcccttcgt agaaatgaac gcgtcccgag agcaccacca
     2641 ccggcgtgcg atcgacgtcg cccagaacga gctccccggc gtggccgacg acgccggacg
     2701 gcaccggaaa gtgtggaatc gcctcgtagg gaatcgcgac ccggttcttc aggcgttcgg
     2761 cgaacgcgcc gagaccggat ccgaggacga gaccgacggc cggcgccaga tcgcgcttac
     2821 cgcgcaggaa gccggccgag gtgtcgagtc ggccgagcag cccggtctcg gtcatccggc
     2881 gagcaatccc gcgatcgtcg ccgtcatgaa cgacgcgagc gagcccccga gaagggcgcg
     2941 gaaaccgagc cgggcgatgt cgcccttgcg tgtcggggcg agtcctccga tccctccgat
     3001 ctggatgccg atcgaggaga agttcgcgaa cccgcacaac gcgaacgacg cgatgaggcg
     3061 cgccttctcc gacattcccc cctggacggt gctcatcttc gcgtaggcga agaactcgtt
     3121 gatgacggtc ttgttcccga tcaggtcggc gacggcgccc gcgtccctgg gggggacccc
     3181 catgacgacc gccagaggcc agaggaccca cgcgaagacc tgctgaagct ggaactccgg
     3241 gtgtccgaag agacccccgg cccagccgag aaaaccgttc aggagcgcga tcagcgcgat
     3301 gaaggcgatg agcatcgcga cgatgttgat gacgagcgtc atcccttgcg acgcgccgga
     3361 cgcggcggcg tcgatcaggt tcgtgtgcgt cttctcgacc t
//
LOCUS       DASWAV010000058         2159 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U2665
            k141_42300, whole genome shotgun sequence.
ACCESSION   DASWAV010000058 DASWAV010000000
VERSION     DASWAV010000058.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960316
            Sequence Read Archive: SRR10260007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2159)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2159)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:47:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,929
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2159
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_42300"
                     /isolate="SMAG_U2665"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.36444 N 132.31889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(172..915)
                     /locus_tag="VF958_01300"
     CDS             complement(172..915)
                     /locus_tag="VF958_01300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9147772.1"
                     /translation="MRRLATAAAAILLTAAVSEATGLDDLRSALEKLRSRDAIRARIT
                     QENSGAYEDEGQTRTRQSRAAIVAEDGGPGQPLKLVYDDAVLAQASREQAGRRDSRGP
                     ADAVRDLDALRVLDLLRPAEKLVDDLAGAQLLSESADRVAGRPARVLELVMRAPRGLD
                     REKGFKVTRTARFWIDAAGAPLASEVRVHTEVRRFIFKVRFDTVEKNEYAVAAHRLMT
                     RRRDTVNHWKAWIIAEGENRSSTTVEVVE"
     gene            complement(912..>2159)
                     /locus_tag="VF958_01305"
     CDS             complement(912..>2159)
                     /locus_tag="VF958_01305"
                     /inference="COORDINATES: protein motif:HMM:NF024910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HEX9147773.1"
                     /translation="VPQALCVTALSSAFPREGGLYAWSKRAFGDFHGFVAGWCYWVNN
                     LFYYPSLALATAGMGLYVFGTRYAHLEQSRAYGMIGSLVLVSIAVAASVVGWRSGKWV
                     QNLGGAANWLPIVFLLVIAALSLARFGSANAIRLASLVPHWTDLALVLAFSKLCFAFA
                     GFELAPLVSEEIVDPRKTIPRAIFAAAVSIAFVYLAGTLALLVSLPTSETSILTGLNQ
                     AVTKSGARVGLAFLGAPTALMMWLAGLGALAAWFAGTGRLLFVAGLDKYLPPAFSRIH
                     PRWNTPAFALVVQGGLAIAFTILATAGAGVKEAYLFFADFTLILYFIPYLYMFASGIA
                     LGREVRESDGAIPIPGGSAGNVVVNVAGFAVTALSIVLSCIPPADEPRKALRVVLLVG
                     GAAAFVAMGMGLYALASRRARRA"
BASE COUNT          389 a          731 c          741 g          298 t
ORIGIN      
        1 cggcaggcgc gcctggacgg tcgtgagcgc ggcgcgaaag gcgttgcgat tcgactcgac
       61 ggaaaaatcc gtcagctgtt cgttgcgaaa ttcggggagc aagacgacct ccggcgtggg
      121 caatctacca gagtggggtc aggtctccac ttgccacata agaccggggc cctactccac
      181 cacctcgacg gtcgtcgacg agcggttctc tccttccgcg atgatccacg ccttccagtg
      241 gttcacggtg tcgcggcgtc gggtcatcag gcgatgcgcc gcaacggcgt actcgttctt
      301 ctcgaccgtg tcgaagcgca ctttgaagat gaaccggcgc acctccgtgt gcacgcggac
      361 ttccgacgcc agcggcgcgc ctgccgcatc gatccagaat cgcgcggtcc gcgtcacctt
      421 gaatcccttc tcgcgatcga gccctcgcgg cgcacgcatg acgagctcga ggacgcgggc
      481 gggtcgcccc gcgacgcggt ccgcggattc cgacagcagc tgcgcgccgg cgaggtcatc
      541 gacgagcttt tcggcggggc gcagcaggtc caggacgcgc agcgcgtcga gatctcgaac
      601 cgcgtcggcg ggtccgcgcg agtcacgccg gccggcctgc tcccgcgacg cctgcgccag
      661 caccgcatcg tcgtaaacga gcttcaacgg ctgcccgggg ccgccgtcct ccgcgacgat
      721 cgccgcccgc gactgccggg tccgagtctg accctcgtcc tcgtaggcgc cggagttctc
      781 ctgcgtgatc cgcgcgcgaa tcgcatcgcg ggagcgcaac ttctcgagcg cggaacgcag
      841 gtcgtcgagc cccgtcgctt ccgaaacggc ggctgtgagc aggatcgccg cggcggcggt
      901 cgcgagtctc ctcatgctct cctcgcccgc ctcgacgcga gcgcgtagag ccccatgccc
      961 atcgccacga acgccgccgc gcccccgacg aggagcacga cacgcagggc ctttcggggc
     1021 tcgtcggcgg ggggaataca cgagagcacg atcgagagcg ccgtcaccgc gaaccccgcg
     1081 acgttgacga cgacgttgcc ggcggaaccg cccgggatcg ggatcgcgcc gtcgctctcc
     1141 cgaacttctc ttccgagcgc gatccccgag gcaaacatgt agaggtaggg gatgaaatac
     1201 agaatgagcg tgaagtcggc gaagaagaga taggcctcct tcacgcccgc acccgcggtc
     1261 gcgaggatcg tgaacgcgat cgcgaggccg ccctgcacga ccagggcgaa cgccggcgtg
     1321 ttccagcgcg ggtgaattcg cgagaacgcg gggggcaggt acttgtcgag tccggcgacg
     1381 aagagcaggc gcccggttcc ggcgaaccac gcggcgagcg cccccagtcc ggcgagccac
     1441 atcatcagcg cggtcggcgc gccgagaaaa gcgaggccga cccgcgctcc ggatttcgtg
     1501 accgcctggt tgaggccggt caggatcgac gtctccgagg tcggcaggga cacgagcagc
     1561 gcgagcgttc cggcgagata cacgaacgcg atcgagacgg cggcggcgaa gatggcgcgc
     1621 gggatggtct ttcgcgggtc gacgatctcc tcggagacga gaggcgcgag ctcgaacccg
     1681 gcgaacgcga aacagagttt cgaaaaggcg aggacgagcg cgaggtccgt ccagtgcggc
     1741 acgagcgatg cgagccggat ggcgttcgcc gatccgaacc gcgcgagcga cagcgctgcg
     1801 atcacgagca gaaacacgat cggcagccag ttcgcggcgc cgccgaggtt ctgaacccac
     1861 ttgcccgatc gccagccgac gacgctcgcg gcgaccgcga tcgacacgag tacgagcgat
     1921 ccgatcatcc cgtacgcgcg gctctgctcg aggtgcgcat atcgcgtacc gaagacgtag
     1981 aggcccattc ccgcggtcgc gagggcgagc gaggggtaat agaagaggtt gttgacccag
     2041 tagcaccagc cggcaacgaa cccgtggaag tcgccgaacg ctcgcttgct ccacgcgtac
     2101 aggccacctt cgcgcgggaa agcggacgag agcgccgtga cacagagggc ctgcgggac
//
LOCUS       DASWAV010000059         2883 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U2665
            k141_122964, whole genome shotgun sequence.
ACCESSION   DASWAV010000059 DASWAV010000000
VERSION     DASWAV010000059.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960316
            Sequence Read Archive: SRR10260007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2883)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2883)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:47:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,929
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2883
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_122964"
                     /isolate="SMAG_U2665"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.36444 N 132.31889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..476
                     /locus_tag="VF958_01310"
     CDS             <1..476
                     /locus_tag="VF958_01310"
                     /inference="COORDINATES: protein motif:HMM:NF017013.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="MOFRL family protein"
                     /protein_id="HEX9147774.1"
                     /translation="RRARLEGEAREVGRCLGRELARAPEGQVLLAGGETTVTLGRVHG
                     RGGRNLEVALAAAEELEGLADAALLAAGSDGRDGSSRAAGAVVDGSTITRARRSGLDP
                     ARALARHDTEPFFERAGGLLRTGPTGTNVADWAFGVRVHRTGKERRETGREKRPK"
     gene            473..1099
                     /locus_tag="VF958_01315"
     CDS             473..1099
                     /locus_tag="VF958_01315"
                     /inference="COORDINATES: protein motif:HMM:NF016176.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HEX9147775.1"
                     /translation="MKKFACVGGLLAVVLGIATAQEHGQPLRYRLEVAGSTVKWQLPS
                     TFETVNGEVPVFHGTIEVSPLPSGAWDVQGRIVVPAAAMRTGNRRRDNRMRGRILETA
                     RYPEIVFELTRFTGDLSRFRAGQNFTAQIAGDLTVHGRVAPVQLPVDVYVFTDHVDVA
                     GSFPLNWKGYGMRDPSIGPIRVKEPMFVDFRLRAVPVTPTAAYHERFR"
     gene            1096..2673
                     /locus_tag="VF958_01320"
     CDS             1096..2673
                     /locus_tag="VF958_01320"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HEX9147776.1"
                     /translation="MKTTSRRQELARLERFTEEPNRFGAYRIALGFPNSYEIGMSNLG
                     FQWVYRLFNRIPDLVCERFFSEEGEPAVTFESGTPLSDFGVLAWSLSWEMDVVNIVRT
                     LSAAGIPLRREARDERHPLLLVGGDIARMNPAVLSPFVDVFALGDGERLVPAIADLAK
                     RGLDRATFLDEIARLPGFFVPAVQGTRAEATESARVVIQQPMSRKEIRPDFEVPHTTI
                     LTPRTELADKLLIEISRGCTEMCRFCWAAYAMAPVKQYPASSILGAARAARPLTDRTG
                     LIATAVCDHPQITEILQGLADLQYHIALSSIKIDAIEDPILEVLARQGERALAIAPEA
                     GNERLRRHINKKVSDEMLREKARLVFARGFTRLKLYLQVGLPSETDEDVADIVRMVGS
                     LREIAIAEGKRSGRVADLVPSVNAFIPKPHTPYEDESLADETVLKEKLSYLQGEFAKM
                     PNVVFRGMPVGEAVWEAYLAKADESAADVLEEAAAGVPVRRLLKAHKGRIDSVVRPTG
                     VPANAPTATPWSFISKR"
     gene            2670..>2883
                     /locus_tag="VF958_01325"
     CDS             2670..>2883
                     /locus_tag="VF958_01325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9147777.1"
                     /translation="MRVRSSRVRVQIGLIGLLALLVPTQGRSVDFPLLKPPATLFRAH
                     REKFLAKLPPNSIAILHATPLKTMSND"
BASE COUNT          480 a          985 c          964 g          454 t
ORIGIN      
        1 cgcggcgagc gcgattggag ggagaggcac gcgaggtcgg ccgctgcctc ggccgcgaac
       61 tcgcgcgcgc cccggagggc caggtcctcc tcgccggcgg cgagaccacc gtcacgctcg
      121 gacgcgtgca cgggcgcggc gggcgaaatc tcgaggtcgc gctcgccgcg gccgaagagc
      181 tcgaaggcct ggccgacgca gcgctcctcg ccgcgggctc cgacggccgc gacggctctt
      241 cgagagcggc gggcgcggtc gtcgacggat cgacgatcac gcgggcgcgc cggtccggtc
      301 tcgatccggc ccgcgcgctc gcacgccacg acacggagcc tttcttcgag cgggcaggcg
      361 ggctccttcg gacgggcccg accggaacaa acgtcgccga ctgggcgttc ggagtacgcg
      421 tccatcggac gggaaaagaa agacgggaaa cgggaagaga gaagcgcccg aaatgaagaa
      481 attcgcgtgt gtcgggggat tgctggcagt cgtgctcgga atcgcgacgg cgcaggagca
      541 tggccagccg ctccgataca ggctcgaggt cgcggggtcc acggtgaagt ggcagctccc
      601 gtcgacgttc gagaccgtca acggcgaggt gccggtcttt cacggaacga tcgaggtgag
      661 tccgctgccg tcgggggcct gggacgtcca gggccggatc gtcgttccgg cggccgccat
      721 gcggaccggg aaccggcgcc gcgacaaccg gatgcgcggc cggatcctcg agaccgcgcg
      781 atacccggag atcgtcttcg agctcacccg cttcaccgga gacctctcgc gcttccgtgc
      841 cggccagaac ttcacggcgc agatcgcggg agacctgacc gtacatggac gcgtcgcgcc
      901 cgtccagctc ccagtcgacg tctacgtctt caccgatcac gtcgacgtgg ccggatcgtt
      961 tccgctcaac tggaagggtt acgggatgcg cgatccctcg atcggcccga ttcgcgtgaa
     1021 agagccgatg ttcgtcgatt tccgcctgcg cgccgtgccc gtgacgccga cggcggcata
     1081 tcatgagcgg ttccgatgaa gacgacgtcg cgccgacagg agctcgcccg cctcgagcgc
     1141 ttcaccgagg agccgaaccg cttcggcgct taccggatcg ctctcgggtt tcccaactcc
     1201 tacgagatcg gaatgtcgaa cctcggattc cagtgggtct atcggctctt caatcggatc
     1261 cccgacctcg tctgtgagcg cttcttctcc gaggaaggcg agcccgccgt cacgttcgag
     1321 agcgggaccc cgctttccga cttcggggtt ctcgcctggt cgctctcctg ggagatggac
     1381 gtcgtcaaca tcgtccgcac tctctccgct gcgggaatcc cgctgcgacg cgaggcgcgc
     1441 gacgagcgcc acccgctgct tctcgtcggc ggcgacatcg cacggatgaa ccccgcggtc
     1501 ctttccccct tcgtcgacgt gttcgcgctc ggcgacggcg aacgcctggt cccggcgatc
     1561 gccgacctcg cgaaacgggg gctcgaccga gcgacgttcc tcgacgagat cgcgcggctc
     1621 cccgggttct tcgtgcccgc cgtgcagggc acgcgcgcgg aggcaacgga gagcgcgcgg
     1681 gtcgtcatcc agcagccgat gtcgcgcaag gagatccggc cggatttcga ggtaccgcac
     1741 acgacgatct tgacgccgcg gacggagctc gccgacaagc tcctcatcga gatctcacgc
     1801 ggatgtaccg agatgtgccg cttctgctgg gcggcctacg cgatggcgcc cgtcaagcag
     1861 tacccggcct cttcgatcct cggtgcggct cgcgcggcgc gacccttgac cgaccgcacc
     1921 ggcttgatcg ccaccgccgt ctgtgaccac ccgcagatca cggaaatcct gcaagggctc
     1981 gcggacctgc agtaccacat cgcgctgtcc tcgataaaga tcgacgcgat cgaggacccg
     2041 atcctggaag ttctcgcgcg acagggcgag cgcgcgctcg ccatcgcgcc ggaggccgga
     2101 aacgagcggc tccgccgcca catcaacaag aaagtctcgg acgagatgct gcgcgagaaa
     2161 gcacgcctcg tcttcgcgcg cgggttcacc cgcctgaagc tctatctgca ggtcgggctt
     2221 ccttcggaga ccgacgagga cgtcgccgac atcgtccgca tggtcgggtc gctgcgggag
     2281 atcgcgatcg cggaaggcaa gcggtccggg cgcgtggccg acctcgtgcc gtcggtcaac
     2341 gcgttcattc cgaagccgca cacgccatac gaggacgaat cactcgccga cgagaccgtg
     2401 ctcaaagaga aactttcgta cctgcaaggg gagttcgcga agatgccgaa cgtcgtcttt
     2461 cgcgggatgc ccgtcggcga agcggtgtgg gaggcgtatc tcgccaaggc agacgagtcg
     2521 gcggccgacg tcctggaaga ggccgcggcc ggagttccgg ttcggcggtt gctgaaggcg
     2581 cacaagggac gcatcgactc cgtcgtgagg ccgaccgggg tgccggcgaa cgctccgacg
     2641 gccactccat ggtcgttcat ttcgaaacgg tgagagtccg gagctcgaga gtcagagtcc
     2701 aaatcggcct cattggcctc ctggctctcc tggtcccgac tcagggtcgg tcggtcgact
     2761 ttcccctcct caagcctccg gccacgctgt tccgggcgca ccgggaaaag ttcctcgcga
     2821 agctgccccc gaactccatc gcgatccttc acgccacgcc gctgaagacg atgtcgaacg
     2881 acg
//
LOCUS       DASWAV010000060         2017 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U2665
            k141_282282, whole genome shotgun sequence.
ACCESSION   DASWAV010000060 DASWAV010000000
VERSION     DASWAV010000060.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960316
            Sequence Read Archive: SRR10260007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2017)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2017)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:47:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,929
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2017
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_282282"
                     /isolate="SMAG_U2665"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.36444 N 132.31889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..365)
                     /locus_tag="VF958_01330"
     CDS             complement(<1..365)
                     /locus_tag="VF958_01330"
                     /inference="COORDINATES: protein motif:HMM:TIGR00427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarC family protein"
                     /protein_id="HEX9147778.1"
                     /translation="MQFAVLAFSSLFSVINPISAAPIFVGLTGGMSAGERRRAALRAC
                     VAAAAILAVFAGAGGAIFSFFGITVPAFQIAGGLLFTLMSFREVQEGESRVRPSDAEK
                     EDPSIVPLGIPVISGPGAIS"
     gene            complement(484..1620)
                     /locus_tag="VF958_01335"
     CDS             complement(484..1620)
                     /locus_tag="VF958_01335"
                     /inference="COORDINATES: protein motif:HMM:TIGR01420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilT/PilU family type 4a pilus ATPase"
                     /protein_id="HEX9147779.1"
                     /translation="MDEVEDINQLIRELNEDRPPRGEPGSTARLEAWLQTLVERAGSD
                     LLLVAGAPPAIRVDGRIVPLPHAPLSGEEIEEAVLPTLSSRGRSRYAQESVADLSFKV
                     RGTGRFRINLHRERGRPAAAIRALPTDPPRLSTLGLPPGAEALTRLPRGLVLVGGPTG
                     SGKTTTLAALVEEINRRDARHIVTIEDPIEYEHTHQSSLVEQVEVGVDAPDFPSALRA
                     AMRQAPDVLVVGEMRDPETMRIALAAAETGHLVFTTVHTTDIASAVSRIADSFPAERQ
                     NTIRQELSMALGAVLTQTLLPRKGGGRAAAAELLIVSYGTRQHIRKNALQHLHQEIAL
                     TKKQGSITLEESLARLAREGLVEVEEARARAVHPEEFENHLRAG"
     gene            1696..>2017
                     /locus_tag="VF958_01340"
     CDS             1696..>2017
                     /locus_tag="VF958_01340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9147780.1"
                     /translation="MRVERAVLPAAAVFLGLTAVSMLFAGGNRAESPPEVAPSNPDGA
                     SNLFLAPDLSFTDDSSANFPIRGENVSDGGIASDRPTVIFFGTAHCWNTNREAERYVA
                     LYRKH"
BASE COUNT          323 a          675 c          695 g          324 t
ORIGIN      
        1 gagatcgccc cgggaccgct gatgacgggg ataccgagcg ggacgatgga gggatcttcc
       61 ttttccgcgt cgctcgggcg aactcgactc tctccctcct ggacttcgcg aaacgacatc
      121 agagtgaaca gcagcccgcc cgcgatctgg aacgcgggca ccgtgatccc gaagaaggag
      181 aagatcgcgc cgcccgcccc cgcgaagacg gcgaggatcg cggccgccgc gacgcacgct
      241 cgcagcgctg cgcggcggcg ctctcccgcg gacatcccac ccgtcaggcc gacgaagatc
      301 ggcgcggcgg agattggatt gatcaccgag aagagcgaag agaatgcgag gaccgcgaac
      361 tgcatcgggt caggtcttca ctgatcgaag cagcgcccga ttcggtcgga tggacttgcc
      421 acttcggtca acgaagtggc aagtggagac ctgaccccct atgtggcaag tggagacctg
      481 accctatccc gctcgcaggt ggttctcgaa ctcttcggga tgcactgcgc gggcgcgcgc
      541 ttcctcgacc tcgacgagcc cctcccgcgc gaggcgcgcg agcgactcct cgagcgtgat
      601 cgagccctgc ttcttcgtga gcgcgatttc ctgatgaagg tgctggagcg cgttcttgcg
      661 gatgtgctgg cgcgttccgt agctcacgat caggagctcg gccgccgcgg cccgcccgcc
      721 ccccttccgg gggagaagcg tctgcgtgag gaccgccccg agcgccatcg agagctcctg
      781 gcggatcgta ttctgtcgct cggccggaaa cgagtccgcg atacgcgaca cggccgaggc
      841 gatgtcggtc gtatggaccg tcgtgaacac gaggtgcccg gtttcggccg cggcgagagc
      901 gatccgcatc gtctcggggt cgcgcatctc gccgacgacg agaacgtcgg gcgcctgtcg
      961 catcgcggcg cgcagtgccg aagggaaatc gggtgcgtcg actcccacct cgacctgctc
     1021 gacgagactc gactggtgcg tgtgctcgta ctcgatcggg tcctcgatcg tgacgatgtg
     1081 ccgcgcgtcg cggcggttga tctcctcgac gagcgccgcg agagtcgtcg tcttgccgga
     1141 gcccgtgggg ccgccgacca ggacgaggcc gcgcggcagg cgcgtgagcg cctcggcgcc
     1201 cggcggaagg cccagcgtgg agagccgcgg cggatccgtc ggcagggccc ggatcgcggc
     1261 agcgggccgt ccgcgctcgc gatgaaggtt gatccgaaag cgacccgtcc ctcggacctt
     1321 gaaggagagg tccgccaccg actcctgcgc gtaacgggaa cgcccgcggg atgaaagcgt
     1381 cggaagaacc gcctcctcga tctcttcgcc cgagagcggg gcgtgcggga gcggcacgat
     1441 ccgtccgtcg acgcggatcg cgggcggcgc gccggcgacg agcagcaggt cgcttcccgc
     1501 acgctcgacg agcgtctgga gccacgcctc cagccgtgcg gtgcttcccg gctcgccccg
     1561 ggggggtcgg tcctcgttca gctcgcggat gagttggttg atgtcctcga cttcgtccac
     1621 ggagtgatat tacgcaaccg gccgccggag cgtcggttcg aatcttccgg acggggcccg
     1681 gcgtaggttc agggcatgag agtggagcgt gcagtcctcc cggccgcagc ggtcttcctc
     1741 ggactgacgg cggtttcaat gcttttcgcc ggcgggaacc gggccgaatc accgcccgag
     1801 gtcgccccct caaatccgga cggggcatcg aacctcttcc tcgcgccgga cctgtcgttt
     1861 accgacgact catccgccaa ctttccgatc cggggagaga acgtgtccga cggcggcatc
     1921 gcctcggatc gtccgacggt gatcttcttc gggacggccc actgctggaa caccaaccgg
     1981 gaggctgaga gatacgtcgc tctctatcgg aagcact
//
LOCUS       DASWAV010000061         7174 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U2665
            k141_723433, whole genome shotgun sequence.
ACCESSION   DASWAV010000061 DASWAV010000000
VERSION     DASWAV010000061.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960316
            Sequence Read Archive: SRR10260007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 7174)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7174)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:47:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,929
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7174
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_723433"
                     /isolate="SMAG_U2665"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.36444 N 132.31889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1805)
                     /locus_tag="VF958_01345"
     CDS             complement(<1..1805)
                     /locus_tag="VF958_01345"
                     /inference="COORDINATES: protein motif:HMM:TIGR02669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIID/LytB domain-containing protein"
                     /protein_id="HEX9147781.1"
                     /translation="MKRGGLLFPGRAAPGALLALALALAAGCASGPPAKPGEKPPSAT
                     AAASPPAERRSEKGARAALGVPQVAAPSGVVYRVGLKSDLVELAYGLPGEPWIVVSGG
                     RAELLRGPIIFRSETTPVGAPAFSVQAAAFSQEEPARRTLEKLSTDLQTAGSVAFSAE
                     RGVYRVLLGNFVTRAEAEALAAKVRSGGQDAIVIEGAAQRAAAPGATISVTSGEAGPR
                     RLASPVEIFPSSGAAAGPANVRFDDKPYRGSLAVIVNPRGTLNLVNRVDLEEYLYGVV
                     PAELGPKRYDEIEALKAQAVAARTYAFAHRAQFDAEGYDLCATAKCQVYAGLALEDPL
                     SNGAVDATRGLVIASGGTFADALFISTCGGRTENVENVFTGEAAPYLVSVECGELPAS
                     SLAGARLLKTTASHGRSGLEWRGYVLERHAPRRRGGRATWVEIAQRWAGIERRAAPAA
                     SLAPASVYPSILAAFDLASARVVHLMPREERYYAEYPSATGRLTGAARDAYAFLLRFK
                     FGAGEPLPSPDRTVSDEEYAGLLFSGALRLLGITEASGKFLSREASNVWVRTPEGRVG
                     LPVDPDLPLARRVGDRWFVASTLALRPGDRLRWWKR"
     gene            complement(1802..2536)
                     /locus_tag="VF958_01350"
     CDS             complement(1802..2536)
                     /locus_tag="VF958_01350"
                     /inference="COORDINATES: protein motif:HMM:NF013828.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX9147782.1"
                     /translation="MEVAPDACSVCGGAGWVLDSVDGRKQARACACRGAALRRERLAS
                     AGIPVRYQGDNFANFDEQTPQLANAKRIAQEFVDSYPVVDSGLLFVGPAGRGKTHLAC
                     AILSELVATKGVVGLYADFSDLLLRIQTSFRPDADSSKESVLNPYADAELLVLDELGA
                     SKPHAWVLDVLYNLLNTRYNRKRITIATSNFEDEPDTASGEREKLEDRIGYRLRSRLY
                     EMCTLVALRGKDYRKEVLGTHVRSRF"
     gene            complement(2564..3043)
                     /locus_tag="VF958_01355"
     CDS             complement(2564..3043)
                     /locus_tag="VF958_01355"
                     /inference="COORDINATES: protein motif:HMM:NF019928.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC domain-containing protein"
                     /protein_id="HEX9147783.1"
                     /translation="MVSPIVRSVSIETSASDAFDAFTRVGELLSWWCEGALVGRRQGG
                     NWAVGFTDDRGGTQATIMGKIEAYEPGQKLAVRDISYEMRAEEPLEGLSFVLTFVEEG
                     GACRVTVEQGIPDAGPAYDRYRGIAGPRWEERLADLKRYLEGPRKRRVLIEAGLPEN"
     gene            3098..5140
                     /gene="argS"
                     /locus_tag="VF958_01360"
     CDS             3098..5140
                     /gene="argS"
                     /locus_tag="VF958_01360"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: protein motif:HMM:NF012952.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HEX9147784.1"
                     /translation="MNGALSLETAQSGIAAAVRDAAERLWGARLERVVLERPPSLEMG
                     DLASPVAFDLAKSLRKAPRAIATDMASAIVLPHGVREVRVEGGGYVNFFLDRGRLVES
                     LLAAEPAPPAARPGKIVVEHTNINPNKAAHIGHLRNAALGDVLARTLTFLGHRVEVQN
                     YLDDTGVQVADVVAGLQHIKGVRSAEDMRRAATGGPLPDGSLHPKGLAYLCWDVYADV
                     AKVYEENEAARGWRAEVLHAIEAGEDETARIAEVLSEAIVSAHLATMGRLGIAYDLLP
                     RESDILRKHFWSRAFELLKRSGAVQREEEGRHAGCWVLRLSESPEFAGMEEPDKILVR
                     SNGTVTYTAKDIAYQLWKFGLLGIDFDYEPFRPEWNSGAVREEEISREVREHPLWRTA
                     HAGGAPDPPDFGRASSVINVIDVRQSYLQKVVREGLRVLGYASEAEKSVHFSYEMVGL
                     TPRAARELADRFQEEYRLSPEDEKKPFVEMSGRKGLGVKIDDLVELLFERSRAEIAAR
                     RRTADDAAEQDARAIAVGALRYFLLKFGRNKLIAFDFDEALNFEGDTGPYLQYSLVRA
                     DNIFRKLEERGLGGDAAEGPIPPEGWDDDLWALVLDAAAIPEVAERAVDSLELSTLAR
                     HAFGLAQAFNHFYHQQPIVNEKDPVVRTRRLVVAKIFRRAMSRLLGLLGIPEPGRM"
     gene            5143..5508
                     /locus_tag="VF958_01365"
     CDS             5143..5508
                     /locus_tag="VF958_01365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019217449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEX9147785.1"
                     /translation="MEVRKVNLAEKFGGFREHWSPKIAGEVNDSYVKLVKFRGDFVWH
                     KHENEDEMFLVVRGRFTMRLRDGDLLLEENEFVIVPKGVEHCPVADEEAEVLLFEPKT
                     TLNTGNVVNERTIEELDRI"
     gene            5508..6380
                     /locus_tag="VF958_01370"
     CDS             5508..6380
                     /locus_tag="VF958_01370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012628052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEX9147786.1"
                     /translation="MANAARRLPENVPGDFYVDETCIDCDTCRRIEPAVFVEGDSFSY
                     VGAQPESDAARRRALMALVACPTGSIGTVSRTDARGAIAAFPEPVAENVSFCGFTSPD
                     SFGAWSWLIERPEGNVLVDSPRAANPLVKEIERRGGVSLLFLTHRDDVADHAQFAKRF
                     GCERVLHERDVTSGTSGVERRLSGDAPVQLAPDLIAIPVPGHTRGHAVLLYREKFLFT
                     GDHLAWSPRRRALTAFRDACWYSWSEQIRSMERLLDFRFEWVLPGHGWRYHAPSPEAM
                     RTEVEKCVKWMKTR"
     gene            6385..6570
                     /locus_tag="VF958_01375"
     CDS             6385..6570
                     /locus_tag="VF958_01375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9147787.1"
                     /translation="MNWIFWLGLAVAIGVIAAVTGAKPRGTRPVSHTHLMGVARFVLV
                     VMVLLFLYLAYRGYAGR"
     gene            complement(6567..>7174)
                     /locus_tag="VF958_01380"
     CDS             complement(6567..>7174)
                     /locus_tag="VF958_01380"
                     /inference="COORDINATES: protein motif:HMM:NF020133.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9147788.1"
                     /translation="NGAGGVEFVRDVETRVLGKPAGVQDYYPAFGGGLHVLRFETGRT
                     VAERRDVDPEEWNRHLTLFDTGAAHSSGMNNWEVFRRRLEGDESVTRALEKVASAARE
                     MASAAEARDFRAMGSALRDEWEARRELAPVVSSPAIEQAIAAALAAGAWGGKACGAGG
                     GGCVVFLSPAERTLEVREALGRLPEGSVLPVRAERRGLEVS"
BASE COUNT         1145 a         2491 c         2466 g         1072 t
ORIGIN      
        1 cgtttccacc agcgaagccg gtcgcccggg cgcagcgcga gcgtcgaggc gacgaaccac
       61 cgatcgccga cacgcctcgc gagtggaagg tcggggtcga cgggaagccc gacgcggccc
      121 tccggcgtgc ggacccacac gttcgaagcc tcacgcgaca ggaacttgcc ggatgcctcc
      181 gtgatgccga gaagccgcaa cgcgcccgag aagaggaggc ccgcgtactc ttcgtcgctc
      241 accgtccgat ccggagacgg cagcggttca ccggccccga acttgaagcg caataggaac
      301 gcgtaggcgt cgcgcgccgc gccggtcagc cgtccggtcg ccgacgggta ctcggcgtag
      361 tagcgctctt cgcgcggcat gaggtgcacg accctggccg acgccaggtc gaacgccgcg
      421 aggatcgagg gatagacgga cgccggagcg agcgacgcgg ccggggcggc gcgtcgctcg
      481 attcccgccc agcgctgtgc gatctcgacc cacgtcgcgc gcccgccccg tcgtcgcgga
      541 gcgtggcgct cgagcacgta gccgcgccac tcgaggccgc tccgcccgtg gctggcggtc
      601 gtcttcagga ggcgcgctcc cgcgagagag ctcgcgggca gctcgccgca ctcgacggag
      661 acgaggtatg gcgcggcctc gccggtaaag acgttctcga cgttctccgt gcggcccccg
      721 cacgtcgaga tgaagagcgc gtccgcgaac gtgccgcccg acgcaatcac gagaccccgc
      781 gtcgcatcga cggcgccgtt ggagagcggg tcctccagcg cgagcccggc atagacctgg
      841 cacttcgccg tcgcgcagag gtcatagcct tcggcgtcga actgcgcgcg gtgcgcgaag
      901 gcgtaggtgc gcgcggcgac cgcctgggcc ttcaaagcct cgatctcgtc gtagcgcttc
      961 ggcccgagct cggccggaac gacgccgtac aggtattcct cgaggtcgac ccggttgacg
     1021 aggttcaagg tcccgcgcgg gttgacgatg acggccaggc tgccgcggta gggcttgtcg
     1081 tcgaagcgca cgttcgcggg ccccgcggcg gccccgctcg acgggaagat ctcgaccggg
     1141 ctcgcgagac ggcgcggtcc ggcctcgccg gaggtgacgg aaatcgttgc gccgggagcc
     1201 gcggctctct gcgccgcgcc ctcgatcacg atcgcatcct gtccgccgga gcggaccttc
     1261 gcggcgagcg cctccgcctc ggctcgcgtc acgaagttcc cgagaagcac gcggtacacg
     1321 ccgcgctccg cggagaaggc aaccgacccc gccgtctgaa gatctgtcga gagcttctcg
     1381 agagtgcggc gagcgggctc ctcctgcgag aacgcggcgg cctggacgga gaacgcggga
     1441 gcgccgaccg gggtcgtctc cgaccggaag atgatgggac cgcgaagaag ctcggcccgc
     1501 ccgccggaga cgacgatcca tggctcgccc ggcagcccgt aggcgagttc cacgagatcg
     1561 ctcttgagac cgacgcggta gacgacgccg ctcggtgcgg cgacctgcgg gacgccaagt
     1621 gcggcgcgcg cgcctttctc cgaccgccgc tcggcgggcg gagacgcggc cgccgtcgcg
     1681 gaggggggct tctcgccggg cttcgccggc gggccgctcg cgcacccggc cgccaacgcg
     1741 agcgcgaggg cgagtaacgc cccgggcgcg gcgcggcccg ggaagaggag tccgccccgt
     1801 ttcaaaaccg cgaccggacg tgcgtcccga ggacctcttt acggtagtcc ttgccgcgga
     1861 gcgcgacgag agtgcacatc tcgtagaggc ggctgcggag tcgatatccg atgcgatcct
     1921 cgagcttctc tctctctccg gacgccgtgt ccggctcgtc ctcgaagttc gaagtcgcta
     1981 tcgtgatgcg cttgcggttg tagcgcgtat tcaacaggtt gtagaggacg tcgagcaccc
     2041 aggcgtgcgg cttggaagcc ccgagctcgt ccaggacgag aagttccgcg tcggcgtacg
     2101 ggttcaaaac ggattccttg gaggaatcgg cgtccggccg gaagctcgtc tggatgcgca
     2161 gcagaagatc cgagaagtcc gcgtagagcc cgacgacgcc cttcgttgcc acgagctcgg
     2221 aaagaatcgc gcacgcgagg tgggtcttgc cgcgtccggc cgggccgacg aagagcaatc
     2281 ccgagtcgac caccgggtag gagtcgacga actcctgcgc aatccgcttc gcattcgcga
     2341 gttgcggcgt ctgctcgtcg aagttcgcga aattgtcccc ctgatatcgc acgggaatgc
     2401 ctgccgacgc gagtcgctcg cggcgcagcg ccgcaccgcg acaagcgcat gcacgcgcct
     2461 gcttccgtcc gtcgaccgaa tcgaggaccc agccggcgcc cccacagacc gaacaagcgt
     2521 cgggcgcgac ttccatcgcg acgattctaa gccgtccgaa agactaattc tcgggcagcc
     2581 ccgcctcgat caggacgcgg cgctttcgcg ggccttcgag gtagcgtttc agatccgcga
     2641 gcctctcctc ccacctcggg cccgcgattc cgcgatagcg atcgtaggcc ggtccggcgt
     2701 cggggatccc ctgctcgacc gtcacccggc atgcgccccc ctcttcgacg aacgtcagga
     2761 cgaaggagag gccctcgagc ggctcctccg cccgcatctc gtaggaaatg tctcgcacgg
     2821 ccagcttctg acccggctcg tacgcctcga tcttgcccat gatcgtggcc tgcgtgccgc
     2881 cgcggtcatc ggtgaaaccg accgcccagt tgcccccctg gcggcggccc acgagggcgc
     2941 cttcgcacca ccaggacaag agctcgccga cgcgcgtgaa ggcatcgaac gcgtcggaag
     3001 cgctcgtctc gatcgacacg gagcggacga tgggcgagac catgagcgca tggtaacgcg
     3061 cggaaagccc ggcctattcg actaacatgc cgcgcccatg aacggcgcgc tctctctcga
     3121 aactgcgcaa tccgggatcg cggcggcggt tcgtgacgcg gccgaacggc tctggggagc
     3181 gcggctggag cgtgtcgtcc tcgaacggcc tccctcgctc gagatgggcg acctggcctc
     3241 ccccgtcgcg ttcgatctcg cgaagtcgct ccgcaaggca ccgcgggcca tcgcgaccga
     3301 catggcgagc gcgatcgtcc tcccgcacgg cgtccgcgaa gtccgagtcg aggggggcgg
     3361 gtacgtgaac ttctttctcg accgcgggcg cctcgtcgag tcgctcctcg ccgcggagcc
     3421 cgcgccgccg gccgcgcgcc ccggaaagat cgtcgtcgag cacacgaaca tcaacccgaa
     3481 caaggcggcg cacatcgggc acctccgcaa cgcggcactc ggagacgttc ttgcgcgcac
     3541 gctcacgttt ctcggccacc gggtcgaagt ccagaactac ctcgacgaca ccggagtgca
     3601 ggtggccgac gtcgtcgcgg ggctgcagca catcaagggt gtccgatcgg cggaggacat
     3661 gcggcgcgcg gcgacgggcg ggccccttcc cgacggctca ttgcacccca aggggctggc
     3721 gtatctctgc tgggacgtgt acgccgacgt ggcgaaagtc tacgaagaga acgaagcggc
     3781 gcggggctgg cgcgccgagg tcctccatgc gatcgaggcc ggcgaagacg agaccgcgcg
     3841 catcgccgaa gtgctctccg aagcgatcgt atcggcccac ctggcgacga tgggacggct
     3901 cgggatcgca tacgaccttc tgccgcggga gagtgacatc ctgcgaaaac acttctggag
     3961 ccgcgcgttc gagctcctga aacgctccgg cgccgtccag cgggaagagg aggggcgcca
     4021 cgccgggtgc tgggtgctgc gcctctccga gtcgcccgag ttcgccggga tggaggagcc
     4081 ggacaagatc ctcgtgcggt cgaacgggac cgtgacgtac acggcgaagg acatcgcgta
     4141 ccagctctgg aagttcgggc tgctcggcat cgacttcgac tacgagccgt tccgccccga
     4201 gtggaactcc ggcgccgtgc gcgaggagga gatttcgcgg gaagtccggg agcatccgct
     4261 gtggcgaacc gcgcacgccg gaggcgcgcc ggaccctcct gacttcggac gcgcgagctc
     4321 cgtcatcaac gtcatcgacg ttcgtcagtc gtaccttcag aaggtcgtcc gcgaaggact
     4381 gcgggtgctc ggctacgcga gtgaagcgga gaagagcgtc cacttctcct acgagatggt
     4441 ggggctcacc ccgcgcgccg cccgcgagct cgccgacaga ttccaggagg agtaccgcct
     4501 ctcccccgag gacgagaaga aaccgttcgt cgagatgtcg ggacgcaagg ggctgggagt
     4561 gaagatcgac gacctggtcg agctcctctt cgagcgctcc cgcgccgaga tcgcggcccg
     4621 gcgccggacc gcggacgacg ccgccgagca ggacgcgcgt gccatcgccg tcggcgctct
     4681 ccggtatttc cttctcaagt tcggccggaa caagctgatc gcgttcgact tcgacgaggc
     4741 cctgaacttc gaaggggaca cggggcccta cctgcagtac tcgctcgtgc gcgcggacaa
     4801 cattttccgg aagctcgaag agcgggggct cggcggcgac gctgcggagg gcccgattcc
     4861 gccggagggt tgggacgacg acctctgggc gctggtcctc gacgcagccg cgatcccgga
     4921 ggtggccgag cgcgcggtgg attcgctcga gctctcgacg ctcgcgcgcc acgccttcgg
     4981 gctcgcccag gccttcaatc acttctacca ccagcagccc atcgtgaacg agaaggaccc
     5041 ggtggtccgt acgcggcgcc tcgtggtcgc gaagatcttc cggcgcgcga tgtcgcggct
     5101 gctcgggctc ctgggcatcc cggagccggg gaggatgtag ccatggaggt tcggaaggtc
     5161 aacctggcgg agaagttcgg gggcttccgc gagcactgga gcccgaagat cgcaggcgag
     5221 gtcaacgact cttacgtcaa gctcgtcaag ttccggggcg atttcgtgtg gcacaagcac
     5281 gagaacgagg acgagatgtt cctcgtcgtt cgcggccgat tcacgatgcg cctgcgcgac
     5341 ggcgacctcc tgctggaaga aaacgagttc gtgatcgttc cgaagggcgt ggagcactgc
     5401 ccggtcgccg acgaggaggc ggaggttctc ctcttcgagc cgaagacgac gttgaacacg
     5461 ggaaacgtcg tgaacgagag gacgatcgaa gagctcgatc gcatctgatg gcgaacgcgg
     5521 cgcggcgcct gcccgagaac gttcccggtg acttctacgt cgacgagacc tgcatcgact
     5581 gcgatacctg ccggcggatc gaaccggcgg tgttcgtcga gggcgattcg ttttcctacg
     5641 tcggggcgca gccggagagc gacgccgcgc ggcggcgagc gctcatggcg ctggtcgcgt
     5701 gcccgaccgg ctcgatcgga acggtgtcgc gcacggacgc tcgcggggcg atcgccgcgt
     5761 tccccgagcc cgtcgccgag aacgtctcgt tctgcggttt cacctcgccc gactcctttg
     5821 gagcgtggag ctggttgatc gagcgcccgg agggcaacgt gctggtcgac tcgccgcgcg
     5881 cggcgaatcc gctggtcaaa gagatcgagc gccgcggcgg cgtctcgctg cttttcctga
     5941 cgcaccggga cgacgtcgcc gaccatgcgc aattcgccaa gcggttcggc tgcgagcgcg
     6001 tccttcacga gcgcgacgtg acgagcggga cgagcggcgt cgagcgaagg ctctccgggg
     6061 acgcgccggt ccagctcgcg ccggacctca tcgcgatccc ggtacccggc cacactcgcg
     6121 gccacgccgt gctcctctac cgggagaagt tcctcttcac gggtgaccac cttgcatggt
     6181 cgccgcggcg ccgcgcgctg accgcgtttc gcgacgcctg ctggtactcg tggagcgagc
     6241 agatccggtc gatggagcgc ctgctcgact tccgcttcga gtgggtgctg cccggccacg
     6301 ggtggcggta ccatgcgccg tcgcccgaag cgatgcggac ggaggtggaa aagtgcgtga
     6361 aatggatgaa gacccggtga cccgatgaac tggatcttct ggctgggtct cgcggtcgcg
     6421 atcggggtga tcgccgcggt gaccggcgcc aagcccaggg ggacacgccc cgtctctcac
     6481 acgcacctga tgggcgtcgc gcgattcgtg ctcgtcgtga tggtgctgct cttcctctat
     6541 ctcgcctacc gcggctacgc cggccgctag gacacttcga ggcctcgcct ctccgcccta
     6601 accggaagga cggagccttc ggggagccgt cccaaggcct cgcgcacctc caaagtccgc
     6661 tccgccggcg agagaaagac gacgcacccg cctccccccg ccccgcacgc ctttccgccc
     6721 cacgcgccgg ccgcgagcgc cgcggcgatc gcctgctcga tcgccggcga tgagacgacg
     6781 ggcgcgagct cccgccgcgc ctcccactcg tctcgaaggg cgctccccat cgcgcgaaag
     6841 tcgcgcgcct cggcggcgct cgccatctcc cgcgcagcgg aggcgacctt ttcgagcgct
     6901 cgcgtgaccg actcgtcccc ctccagccgg cggcggaaca cctcccagtt gttcatcccc
     6961 gaggagtgcg cggcgccggt gtcgaacaga gtgaggtgcc ggttccactc ctccgggtcg
     7021 acgtcgcgcc gctcggcgac cgttcgcccg gtctcgaacc ggaggacgtg gaggccgccc
     7081 ccgaacgccg ggtagtagtc ctggactccc gcgggctttc cgagcacgcg cgtctcgacg
     7141 tcgcgcacga actcgacgcc gcccgcgccg ttca
//
LOCUS       DASWAV010000062         2753 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U2665
            k141_402976, whole genome shotgun sequence.
ACCESSION   DASWAV010000062 DASWAV010000000
VERSION     DASWAV010000062.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960316
            Sequence Read Archive: SRR10260007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2753)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2753)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:47:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,929
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2753
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_402976"
                     /isolate="SMAG_U2665"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.36444 N 132.31889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            120..2153
                     /locus_tag="VF958_01385"
     CDS             120..2153
                     /locus_tag="VF958_01385"
                     /inference="COORDINATES: protein motif:HMM:NF024743.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4097 family beta strand repeat-containing
                     protein"
                     /protein_id="HEX9147789.1"
                     /translation="MSRRAGHTDRVVLWLLLAIGAALMLLAAGTARGEETANRTDRIS
                     ADLPSGATLRLDNVSGDVIAVPGREFTAVATIAVAAQDRKRAEELLGRIRIVQSREGD
                     DLSIETRWPDSRWRFERMDRRTGRRSVFARCEDCRINARYDVTIPPGVTAILGTVNGD
                     VRVRDLDGNLSLRTVNGKVEATGVRRSLEAESVNGDVIGQAVATPAGASYDLETVNGA
                     VRLTLPKEARFALSASTMHGSIASTFALPRAEGADAGELLHRKHEVRIERKPVRKVIV
                     READGEEDTVVVDLKELEKDLEESMKNVDVEIREAMRSVQDSARAGVADGARGGARGG
                     VRDGVRGFRILDPRRSYSGRIGDGGARVRLSALNGSILLLASGSRPEDARPVVSERRS
                     FAVTIPEIRVRVPEVKVDVPAVNVRVPEVRVDTPEVRVVVPQPVVQVHPHAMPRAVWA
                     IDAPVERGDVAGDFLATSGTSSYRIGNVSGRVKILTHSGEITVASAGADADLKTFGGD
                     IRLGAVKGDLAAQTLAGDVRAAAVAGSARVETSGGDIRIERVDGWLRARTAGGDIVVP
                     LVGGHVDAVTAGGDVRIAVAARQPREGISIVNGGGDVALMLPSDFKGDLELTVTEADP
                     SEPAVLSEFPDVSISRKDRIVRATGSLNGGGEKVRVQTSSGTIRLRRGPAVVK"
     gene            2349..>2753
                     /locus_tag="VF958_01390"
     CDS             2349..>2753
                     /locus_tag="VF958_01390"
                     /inference="COORDINATES: protein motif:HMM:NF024245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HEX9147790.1"
                     /translation="MRSRARAILGTGLVLFLLLPAVLASAAEKGTSAKVDINSASQSE
                     LESLPGVGPALAKRIIENRPYSSASGLSKAGVPEGTIDKLRGLVKAGRGSKAEAKEEK
                     IAAKSEKKAQAEKSSATKAERKAPESAPTGKVD"
BASE COUNT          461 a          879 c          999 g          414 t
ORIGIN      
        1 tccgagaacg gcttcgtccg agcccgggcc gcgtcggccc tgaaggcgat cgacctgtga
       61 gaacgcgtaa ccccgatcga ggcggggcgt ctctccttgt gatgagcact ccgcgcgaca
      121 tgagcaggcg agcgggtcac acggaccgcg ttgtcctctg gctgctcctc gcgatcggcg
      181 ctgccctgat gctcttggcg gcgggcacgg cacgcggcga agagacggcg aatcggacgg
      241 atcggatctc ggcggacctg ccgtcggggg caaccctgcg gctcgacaac gtttcgggcg
      301 acgtcatcgc ggtcccgggc cgggagttca cggcggtcgc gaccatcgcc gtcgcggccc
      361 aggaccgcaa gcgagcggag gagctcctcg ggcgcatccg gatcgtccag tctcgcgagg
      421 gcgacgatct ctcgatcgaa acgcggtggc ccgacagccg gtggcgcttc gagcgaatgg
      481 atcgccgcac cggtcggcgc agcgtcttcg cgcggtgcga ggactgccgg atcaacgcgc
      541 ggtacgacgt gacgattccg ccgggggtca cggcgatcct cgggaccgtg aacggggacg
      601 ttcgcgtccg cgacctcgac ggaaatctca gtctccgcac ggtcaatgga aaagtcgagg
      661 cgaccggcgt gcgccgctct ctcgaagccg agtcggtcaa cggtgacgtc atcggccagg
      721 ccgtggcgac gcccgcgggc gcgtcctacg acctcgaaac cgtgaacggc gcggtccgtc
      781 tgacgttgcc caaggaggcg cgattcgccc tctccgcttc gacgatgcac ggatcgatcg
      841 cgtcgacatt cgcgctgccg cgcgccgaag gcgccgacgc cggggagctt ctccacagga
      901 agcacgaggt aaggatcgag agaaagccgg tgcggaaggt catcgtccgc gaggcggacg
      961 gcgaggaaga caccgtcgtc gtcgacctga aggagctcga aaaggacctc gaggagtcca
     1021 tgaagaacgt ggacgtcgaa atccgggagg caatgcgctc cgtccaggat agcgctcgcg
     1081 ccggcgtagc agatggcgcc cgcggcggcg ctcgcggcgg cgttcgcgac ggagtccgag
     1141 gatttcggat cctcgatccg cgacggtctt attcgggtcg gatcggcgac ggcggcgccc
     1201 gggtgcgtct ctcggcgctg aatggctcca tccttctcct cgcgagcggg agccggcccg
     1261 aagacgcccg cccggtggtc tcggaacgtc gctcgttcgc ggtcacgatt ccagagatcc
     1321 gggtgcgagt gcccgaggtg aaggtcgacg ttcccgcggt caatgtgcgg gtccccgagg
     1381 tccgcgtgga cactccggaa gtgcgcgtcg tcgttcctca gccggtcgtg caggtccatc
     1441 ctcacgccat gccgcgggcg gtgtgggcga ttgacgcgcc ggtcgagcgt ggcgacgtcg
     1501 ccggcgactt cctcgcgacg agcgggacgt cgagctaccg gatcggaaac gtatccgggc
     1561 gcgtgaagat tctgacgcac tcgggcgaga tcacggtcgc gtccgcgggc gcggacgccg
     1621 atctgaagac gttcggcggc gatattcgcc ttggtgcggt gaagggcgac ctcgccgccc
     1681 agaccctcgc cggcgacgtg cgcgcggcgg cggttgccgg atcggcccgg gtggagactt
     1741 cgggcggcga catccgcatc gaacgggtcg acggttggct ccgcgcccgg acggcgggag
     1801 gagacatcgt cgtgccgctc gtcggtggac acgtcgacgc cgtgacggcc gggggtgacg
     1861 tccgcatcgc ggtcgccgcc cggcagccgc gggagggaat ctcgatcgtc aacggcggcg
     1921 gggacgtggc gctgatgctc ccctccgact tcaagggtga cctcgagctc acggtcacgg
     1981 aggccgatcc ctccgagccg gcggtcctct cggagtttcc cgacgtctcg atctcacgaa
     2041 aagatcgaat cgtgcgggca acggggtctc tgaacggcgg cggggagaag gtgcgcgttc
     2101 agacgagctc cggcacgatc cgcctgcgcc gcggcccggc ggtggtgaag tagcgcatcg
     2161 cctgacgaga gttctccgag ccgcgtcgat agaagagacg cagcctcctt ttcggcgcga
     2221 cgcgagtcgc gccctttttt cctggggatt cacccgcggt tcatgtcgac gggagtcaga
     2281 tgtcggcctc ggcgcgaccg cgccatgaaa acggcgaaga aagagtcgtg agcggaagga
     2341 gacgaatcat gaggagtcga gcgcgcgcca ttctcgggac ggggctggtc ctattcctgc
     2401 tcctgccggc cgttctcgcg tcggccgcag agaaggggac gtccgcaaag gtcgacatca
     2461 attcggcttc ccagagcgag ctcgaatccc ttccgggagt cggcccggcg ctggcgaaga
     2521 gaatcatcga aaaccgaccg tattcgtcgg cttccggcct ctcgaaggcc ggagttccgg
     2581 agggcacgat cgacaagctt cgcggcctcg tcaaggccgg ccgcgggtcg aaggcggagg
     2641 cgaaggaaga gaaaatcgct gcgaagtccg agaagaaagc ccaggcggag aagagctccg
     2701 cgacgaaggc cgagagaaag gctcccgaga gcgcgcccac cggcaaggtc gat
//
LOCUS       DASWAV010000063         5101 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U2665
            k141_203171, whole genome shotgun sequence.
ACCESSION   DASWAV010000063 DASWAV010000000
VERSION     DASWAV010000063.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960316
            Sequence Read Archive: SRR10260007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 5101)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5101)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:47:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,929
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5101
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_203171"
                     /isolate="SMAG_U2665"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.36444 N 132.31889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1924
                     /locus_tag="VF958_01395"
     CDS             <1..1924
                     /locus_tag="VF958_01395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9147791.1"
                     /translation="VQVSSAGSSPAAASATVNIVQSGPPHPSAGYTITGATQNQFNGN
                     WEAEAGQALTFRATEADPGATFSWVSDGPTSPSGPFSTNPATFAFTGPGSRTVNLTVT
                     GSGTVTAGTTSTTIRIAVTPPRFQALMIPGAGHIDPAPPATDGTWATDLSVTNPGTQP
                     MTVTLDYEPFGTTFPADLSQIQFDSRNSAVLAAGQSLSKTDIVTQLVPPKTGRGVLIL
                     KFEGGNADPIATATVYFTQQGRSFGSSLPTFKVGPYGTATTQSAELSNDQTLVGLRND
                     ANYRFNVSLFNASGQSGFFRLTAFGEDGVQIPIRGGSETGAMDVPIGAFQQSLLQAAD
                     LGLDDATKRYVLKASPVSSTTSLIAAASALDRRNNDLVQVTDDTPRIAAAADTLIDYF
                     IPGVGRIGRDDVADGPHWKTDLTLYSNSSLPRDMTFEYRYTDRNGVEQRVLAPVTVGS
                     GRSMVLDDVVGTVLDGYTPVDLKQESTQGVLRISYRAASDSATAPLLIGGRIYDDRGS
                     AGTAGMQLFVYSNGESVAPGSPLVLPGAQQNLRFRTNIGFFAMGDLPTSVRVTAVKQD
                     GTTAGSLDFILNETGRSGHYAQLPMSAIPGILGEPMTVRVEVLDGSRIGAYVVTVDQI
                     SSDTVFVQGRPTRMVN"
     gene            2055..3179
                     /locus_tag="VF958_01400"
     CDS             2055..3179
                     /locus_tag="VF958_01400"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM/SPASM domain-containing protein"
                     /protein_id="HEX9147792.1"
                     /translation="MSQREIEPTRPAPPGAVSALLEAETVSPYLHERGTVLYNPTTGE
                     SVEKESPDYRALSRIRQGLPSEAAPEVLEHLRAARFLIEDLDSETRRSHLLYVSLETC
                     TSCNHRCPFCPVSVDPREREVMSQQLFESIADQVVQLGGRGVVVFLSNYNEPTIDPLF
                     EERCLALFARGLPVSLLTNASQLDPERADRLARAGRFRYIGINLPTLDPERYLRMHGT
                     RDLARVLGNIDALRAREIAEETAIVVLGDEDEPHRRDVRDIRERFEPLGWEVKPFRIR
                     SRPASGTFVPEPPPVKELRGCELMGSRPFEHLHVTATGKAVLCCQDYYEKLTVGDLKT
                     ETVAEILGGDVMARLRRWTYGVEEAPDDFLCRRCEFALGR"
     gene            3202..4467
                     /locus_tag="VF958_01405"
     CDS             3202..4467
                     /locus_tag="VF958_01405"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX9147793.1"
                     /translation="MTERLAIRADSISKVYRLYDSPLDRLRELLFRAPRHRPFHALKN
                     VSFEIASGGALGLIGENGAGKSTLLKVVAGTTRATSGSIERHGVVASILELGMGFHPE
                     FTGRENARMNAALLGLTGGEIRRRLPEIRDFAELGEFFDRPVRTYSSGMALRLAFAVA
                     THADADVLIVDEALAVGDGYFQKKSVDRITDFQKRGGTLLFCSHALYYVALLCDRAIW
                     LKNGDVAASGPALAVVRAYEAFLQQKERERSAPDESAPVPSRTDGRRPARLTEVLIHD
                     GSGFPRAEFGSGETVAVDLAFESDDPGLAFQLRVGADREDGVQAFAVDTRAEPFAPLT
                     GRRSYRLRLVFPQLPIASGDFRLYAFLGDETALHLHDARVLRPGFTVVSPDYTVGLVK
                     PQHFWTVTERSPEEALPLTAGSPSAPRPA"
     gene            4473..>5101
                     /locus_tag="VF958_01410"
     CDS             4473..>5101
                     /locus_tag="VF958_01410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9147794.1"
                     /translation="MGRIAFLSIGAEERPGGYVRRLGRLRWTRRFPKRVPVELEGDRE
                     ATRAFAAGDDWIVVRDETALPIRGNEIAPAAGHVVVACARPPGSLPVVHTLAEFESVS
                     FPSAGGVPNAAPPAALGFSVSDFPPAAGESVRDYVERLASSATPRVPARGFEALVFED
                     SFAERPEIVRRLPRGVRRLLDVGCGTGAVSAAVRRENPGVEITGIERQSS"
BASE COUNT          787 a         1787 c         1688 g          839 t
ORIGIN      
        1 ggtgcaggta tcgagcgcgg gaagttcccc cgccgcggcg tccgcgaccg tcaacatcgt
       61 ccagagcgga ccgccgcatc cgtcggcggg ctacacgatc acgggcgcga cgcagaatca
      121 gttcaatggg aattgggagg ccgaggcggg ccaggcactg acgttccggg ccaccgaggc
      181 cgatcccggc gcgacgttct cctgggtgtc ggacggcccg accagtccgt cgggaccctt
      241 ctcgaccaac ccggcgacgt tcgctttcac gggcccgggg tcgcgaacgg tgaacctgac
      301 ggtcaccggc agcggcacgg tcaccgcggg aacgacgtca acgacgattc gcattgccgt
      361 gactccgccg cgattccagg cgctcatgat ccccggagcg ggccacatcg acccggctcc
      421 tccggcgacc gacggaacct gggcgacgga cctctcggtc acgaacccgg gcacgcagcc
      481 gatgacggtc acgctcgact acgagccgtt cggtacgacg ttcccggccg acctgtcaca
      541 gatccagttc gactcccgca acagcgcggt gctcgcggcc ggccagtcgt tgtcgaagac
      601 cgacatcgtg acgcagctgg tcccgccgaa gaccggacgg ggagtgctca tcctgaagtt
      661 cgaagggggc aacgccgatc ccatcgcgac ggcgaccgtc tatttcacac agcagggccg
      721 ttcgtttggc tcgtcgcttc ccacgttcaa ggttggcccc tacggcacgg cgacgacgca
      781 gtcggccgag ctctcgaacg accagacgct cgtgggcctt cgcaatgacg cgaactatcg
      841 attcaacgtg tctctcttca acgcttcggg acagagcggg ttcttccggc tgaccgcgtt
      901 cggagaggac ggcgtccaga ttccgattcg gggaggcagc gagacgggcg caatggacgt
      961 gccgatcggc gctttccagc agtcgcttct gcaggcggcc gacctgggtc tcgacgacgc
     1021 gacgaagcgt tacgtgctca aggcgtcgcc cgtgtccagt actacgtctc tcatcgctgc
     1081 ggcttcggct ctcgaccggc gcaacaacga tctcgtccag gtcaccgacg acacgccgcg
     1141 gatcgccgcg gcggccgata ccttgatcga ctacttcatc cccggcgtgg gccgcatcgg
     1201 acgcgacgac gtcgcggacg gcccgcattg gaagacggac ctgacgctct acagcaacag
     1261 cagcctgcct cgcgacatga cgttcgagta ccgctacacc gaccgcaacg gggtcgaaca
     1321 gcgcgtgctc gcgcccgtga cggtcggctc cggccgttcg atggtcttgg acgacgtcgt
     1381 cggaaccgtt ctcgacggct acacgcccgt ggacctgaag caggaatcca cgcagggtgt
     1441 cctccgaatc tcctatcgcg ccgcttcgga ttcggcgacc gcgccgctcc tgatcggcgg
     1501 ccgcatctac gacgaccgcg gttcggcggg gaccgccggc atgcagctct tcgtctactc
     1561 gaacggcgag agcgtcgcgc ccggcagccc gctcgtgctc cccggcgcgc agcagaacct
     1621 gcgcttccgg acgaacatcg gcttcttcgc gatgggcgat ctgccgactt cggtgcgggt
     1681 caccgcggtc aagcaggacg gcacgacggc gggatcgctc gacttcatcc tgaacgaaac
     1741 cggccgctcc gggcactacg ctcagctccc gatgtcggca atcccgggca tcctcggaga
     1801 accgatgacc gttcgcgtcg aggtgctcga cggcagccgg atcggcgctt acgtggtcac
     1861 ggtggaccag atcagctccg acaccgtctt cgtccagggg agaccgaccc gcatggtgaa
     1921 ctgagacgtt tcgaccgatt gatcgaaacc ccggccgccc ggccggggtt tctttttagg
     1981 gacaaggaca ggttgaagaa cgtaagaacg ggcggagcgg gacggtacaa tgcccgttcg
     2041 accgccgccc gagcatgagc caacgcgaaa tcgagccgac ccgcccggcg ccgcccggcg
     2101 ccgtctccgc tctcctggaa gcggaaacgg tctctccgta cctgcacgag cgcggcacag
     2161 tcctctacaa cccgacgacg ggcgaatcgg tcgagaagga gagcccggac taccgcgcgc
     2221 tctcccggat ccgccagggt cttccgagcg aagccgcgcc ggaggttctc gaacacctgc
     2281 gcgcggcgcg gttcctgatc gaggacctcg actccgaaac gcggcggtcg catttgctct
     2341 acgtctcgct cgagacatgc acgagctgca accatcgctg ccccttctgt ccggtctcgg
     2401 tcgatccgcg cgagcgcgag gtgatgagcc agcagctctt cgagtcgatc gccgaccagg
     2461 tggtccagct cggcggccgg ggagtcgtcg tcttcctctc gaattacaac gagccgacga
     2521 ttgacccgct cttcgaggag cgatgcctcg ccctcttcgc gcgggggctg ccggtctcgc
     2581 ttctgaccaa cgcgtcgcag ctcgaccccg aacgcgccga ccgtctcgct cgcgccggtc
     2641 gttttcgcta catcgggatc aacctgccga cgctcgaccc cgagcgctac cttcgcatgc
     2701 acggcacgcg tgatctcgcg cgggtgctcg gcaatatcga cgcgctccgc gcccgggaga
     2761 tcgccgagga gaccgcgatc gtcgtgctcg gagacgaaga cgagccgcac cgcagagacg
     2821 tccgtgacat ccgggagcgg ttcgagccgc tcggctggga ggtcaagccg ttccggatcc
     2881 ggagccgccc cgcgagcggc acgttcgtcc cggaaccgcc gcccgtcaag gagcttcgcg
     2941 gctgcgaact gatggggtcg cgtcccttcg aacacctcca cgtcaccgca acggggaagg
     3001 cggtgctctg ctgccaggac tattacgaga aactgacggt gggggacttg aagaccgaga
     3061 ccgtcgccga gatcctcggc ggcgatgtga tggcccgact gcggcgctgg acttacggcg
     3121 tcgaggaagc gccggacgat ttcctctgtc gccggtgcga gttcgcgctc gggcggtgat
     3181 cgcaggagag gacggacggc gttgacggaa cgccttgcca tccgggccga ctcgatctcg
     3241 aaggtctacc ggctctacga cagcccgttg gaccgccttc gggagctact gtttcgcgcg
     3301 ccgcgccacc gccctttcca cgcgctcaag aacgtctcgt tcgagatcgc ttccgggggc
     3361 gcgctcggcc tgatcggcga gaacggagcg ggcaagtcga cgctcttgaa agtggtcgcg
     3421 ggcacgacgc gggcgacttc ggggtcgatc gagcgccacg gagtcgtcgc gtcgatcctg
     3481 gagctcggga tgggctttca tcccgagttc accgggcgcg agaacgcgcg gatgaacgcg
     3541 gcgctcctcg ggctcaccgg cggagagatc cggcggcgtc tgccggagat ccgcgacttc
     3601 gcggagctcg gtgagttctt cgatcgcccc gtgcggacgt actcatcggg catggcgctg
     3661 cgtctcgcgt tcgccgtcgc cacgcacgcc gacgccgacg tgctcatcgt ggacgaggcg
     3721 ctcgcggtgg gcgacggcta ctttcagaag aagtccgtcg accggatcac cgacttccag
     3781 aagcgcggcg ggactctcct gttctgctcg cacgccctct actacgtggc gctcctctgc
     3841 gatcgcgcga tctggctgaa gaacggggac gtcgcggctt cggggcccgc gctcgcggtc
     3901 gtgcgcgcgt acgaggcatt ccttcagcag aaggagcgcg agcgttccgc gccggacgag
     3961 tcggcacccg tcccctcgag aacggacgga aggcgtcccg cccggcttac cgaggttctc
     4021 atccacgacg gttccgggtt tccgcgcgcg gagttcggct ccggagaaac ggttgccgtg
     4081 gacctcgcgt tcgagagcga cgaccccggt ctcgctttcc aactgcgggt gggcgccgat
     4141 cgcgaggacg gggtgcaggc gtttgcggtg gacacccgcg ccgaaccgtt tgccccgttg
     4201 accgggcgcc gcagctaccg actgcgcctc gtgttcccgc agctcccgat cgcgtcgggc
     4261 gacttccgcc tgtacgcgtt tctcggggac gagacggccc tccacctcca cgacgccaga
     4321 gtgctgcgtc cgggctttac ggtcgtgtcg ccggactaca cggttggact cgtcaagccg
     4381 cagcacttct ggacggtcac ggagaggagt ccggaagagg cgctcccgct gactgccgga
     4441 tcgccttccg ctccccgacc cgcttgactt ctgtgggtcg gatcgccttc ctctcgatcg
     4501 gggcggagga gcgtcccggc ggttacgtcc gccgcctcgg ccgcctgcgc tggacccggc
     4561 ggttcccgaa gcgcgtcccc gtcgagctcg agggcgatcg tgaagcgacg cgggcattcg
     4621 ccgccggcga cgactggatc gtcgtgcgcg atgaaaccgc tcttccgatt cggggcaacg
     4681 agatcgctcc ggccgcaggg cacgtggtcg tcgcctgcgc ccgacctccc ggctctcttc
     4741 ccgtcgttca cacgctcgcg gagttcgaga gcgtgtcctt cccctccgcg ggaggggtcc
     4801 cgaacgccgc cccgcccgct gcgctggggt tctccgtgtc cgactttccc ccggcggccg
     4861 gggagtccgt ccgcgactac gtggagcggc tggcctcttc ggcgacgccc cgcgtccccg
     4921 cgcgcggttt cgaagccctc gtattcgagg actcctttgc cgagcggccg gagatcgttc
     4981 gtcgcctgcc ccgcggggtc cggcgcctgc tcgacgtcgg ctgcggcacc ggggcggtct
     5041 ccgcggccgt ccgccgcgag aaccccggag tcgagatcac cggcatcgag cgccagagct
     5101 c
//
LOCUS       DASWAV010000064         3961 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U2665
            k141_562922, whole genome shotgun sequence.
ACCESSION   DASWAV010000064 DASWAV010000000
VERSION     DASWAV010000064.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960316
            Sequence Read Archive: SRR10260007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 3961)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3961)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:47:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,929
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3961
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_562922"
                     /isolate="SMAG_U2665"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.36444 N 132.31889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..604)
                     /locus_tag="VF958_01415"
     CDS             complement(<1..604)
                     /locus_tag="VF958_01415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015894588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LON peptidase substrate-binding
                     domain-containing protein"
                     /protein_id="HEX9147795.1"
                     /translation="MTESHNLPVVPMRNSVLFPGVGLPINAGRPATLRAIEAAVNSSD
                     KRVFAVAQRQDGDDVAPENLYTIGTIATIGSLQRGLGGVRLLLEGQARGIALRYAPNK
                     GYLEATVQEAREMLPLEARDPAFMALHREARERAAELGQKLGLPEETVNQVLAEVADP
                     GKLADLVAGYIDIPPAERQALLETLAVEDRLRRVLVHVQRQ"
     gene            876..1526
                     /locus_tag="VF958_01420"
     CDS             876..1526
                     /locus_tag="VF958_01420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009151361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HEX9147796.1"
                     /translation="MSAAARRPLKTRDRLWARSLARRLAGAGVRPNWVSGASVFFAAA
                     GGALLALSAERSEATRSVFLLAGAACAQLRLLCNLLDGLLAVEGGLGSPAGEVWNDLP
                     DRLADPLILVGAGLATRILPGGLTLGWLAAILALLTAYIRVLGGSVGVPQDFSGPMAK
                     PHRMAAITVGCIGGAVESAFGRPPRILYLALAAIVAGAAFTAVRRTTRIVRELRTR"
     gene            1507..2163
                     /locus_tag="VF958_01425"
     CDS             1507..2163
                     /locus_tag="VF958_01425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007332043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HEX9147797.1"
                     /translation="MSSGPGDRVIAAILASIARVVTGAAVFYLDKQPDDRQRIYFANH
                     TSHLDFVVLWSALPANVRPLVRPVAGRDYWEKGALRRYLARRVFRAILIDRARRAVDG
                     EATLSIAAARRTIDRLAISMGDRDSIIVFPEGTRGSGEEIAPFKSGIYHLCREKPGIE
                     LVPVYLDNMNRILPKGEMLPVPMLSRVIFGRPMTLEPGETKPAFLSRARAAVQELSNL
                     "
     gene            2160..3107
                     /locus_tag="VF958_01430"
     CDS             2160..3107
                     /locus_tag="VF958_01430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004924405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HEX9147798.1"
                     /translation="MTGLSDRALVRLVGGVLVLLVVATAVGLALSRRARTEEARATIA
                     NLNARIRAWWAMVVIFTIAVWTGGIGSIVLFALMSLLALREFVTLAPTRREDHRTLLW
                     SFFAVLPLQYYLIAIRWYGLFAIMIPVYAFVLVAVSNVIAGRTDRFLERTATIHWGLM
                     VCVYCVSHAPALLILDIPGYRTNAKLLVFLVLVVQISDVLQYVFGKLFGKHPIAPSIS
                     PNKTWEGFVGGVATASLVGTAIWWATPFTPLVALAMSVAVTLAGFAGGLVMSAIKRDR
                     GVKDYGALIQGHGGMLDRIDSLCFAAPVFFHLTRFFFTP"
     gene            complement(3167..>3961)
                     /locus_tag="VF958_01435"
     CDS             complement(3167..>3961)
                     /locus_tag="VF958_01435"
                     /inference="COORDINATES: protein motif:HMM:NF024118.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEX9147799.1"
                     /translation="EAGEQKFLFDAGRGALQRLMQSNVRWQDVKGVFFTHLHSDHVVG
                     FPDLWLTGWLVVPGRSVPLRVWGPRGTRKMMSHLERAYESDIQVRLQNDRAAPEGVVL
                     EVEDIREGVVYESGGAKVTAFEVDHPPVKPAFGYRVDYSGRSVVLSGDTRFSENLIRY
                     ARGVDVLVHEVIAPEALQQSGLSPERAKRIVAYHTTPEQAAEVFSRAKPRLAVYSHIV
                     PPTASEQSLLQPTRRTYAGPLELGEDLMVIEVGQKIEVRRSVRPSS"
BASE COUNT          579 a         1383 c         1288 g          711 t
ORIGIN      
        1 tctgccgctg cacgtgaacg agcacgcggc gcaggcggtc ttcgaccgcc agggtctcga
       61 ggagcgcctg gcgctcggcc ggcggaatgt cgatgtagcc ggcgaccagg tccgcgagct
      121 ttccgggatc ggcgacctcg gcgagaacct ggttcaccgt ttcctcggga agcccgagct
      181 tctggccgag ctccgcggcg cgctcgcgcg cctcgcgatg cagtgccatg aaggcgggat
      241 cgcgggcctc gagcggaagc atctcgcgcg cttcctgcac ggtcgcctcg aggtacccct
      301 tgttcggcgc gtaccgcagc gcgattccgc gagcctgccc ctcgaggagg aggcgaacgc
      361 ctccgagacc ccgctggagc gaaccgatgg tcgcgatcgt tccgatcgtg tagaggttct
      421 ccggagcgac gtcatccccg tcctgtcgct gcgcgaccgc gaacacccgc ttgtccgaag
      481 agttcaccgc cgcttcgatc gcgcgcagcg tggccgggcg gccggcgttg atcggaaggc
      541 cgacccccgg aaacaacacc gagttgcgca tcggtacgac cggcaggttg tgcgactcag
      601 tcatcaggat cttcctttca ttccgcatgg actgcggggg tggaggtccc tgggaccgtc
      661 cccgcgagaa ctgcgacggg tgcaggtcct tgagacctgt ccccttcaaa atacgggccc
      721 ggccgccgct cgggttcgat tccgctcgag cgacccggtc accgtgatgt tgccggtatc
      781 tctcgccatc caaaacatcc gtctattccc gccgattcca gagcggtgcc ggcccgttgt
      841 ggcagtatct cccgacgttc cctggagatg agctcttgag cgcggccgcc cgacgacctc
      901 tgaaaacgcg cgaccgcctc tgggcccgct ccctcgcgcg gcggttggcg ggcgccggcg
      961 tccgcccgaa ttgggtttct ggagcgagcg tcttcttcgc ggcggccggc ggagcgctcc
     1021 tcgcgctctc ggccgagagg agcgaagcga cccgcagcgt gttccttctc gccggggccg
     1081 cgtgcgcgca gcttcggctc ctgtgcaacc tgctcgacgg ccttctggcg gtggaaggcg
     1141 gcctgggaag ccccgccggc gaagtctgga acgacctgcc ggaccgactc gcggacccgc
     1201 tcattctcgt gggagcgggc ctcgcgaccc ggattctgcc gggtggactc acgctcggat
     1261 ggctcgccgc gatcctcgct ctgctgacgg catacatacg cgtgctcggc ggctcggtcg
     1321 gtgttccgca ggacttctcg ggccccatgg cgaagccgca ccgcatggcc gcgatcacgg
     1381 tcggctgcat cggaggggcc gtcgaaagcg cttttggccg ccctccccgg attctgtacc
     1441 tcgcgctcgc cgcgatcgtc gcgggcgccg ccttcaccgc cgtgcgaagg acgacccgga
     1501 tcgttcgtga gctccggacc cggtgatcgc gtgatcgccg cgattcttgc ctcgatcgcg
     1561 cgggtcgtga ccggcgcagc ggtcttctac ctcgacaagc agccggacga tcgccagagg
     1621 atctatttcg cgaaccacac gagccacctc gacttcgtcg tcctctggtc ggcgcttccc
     1681 gcaaacgtcc ggccgctcgt gcgacccgtc gcaggaaggg actactggga gaagggagcg
     1741 cttcggcgct acctggcgcg cagagtcttc cgcgcgatcc tgatcgaccg tgcgcgacgt
     1801 gccgtcgacg gcgaggcgac gctctcgatc gccgcggcgc gccgcacgat cgatcggctc
     1861 gcgatcagca tgggggaccg cgactcgatc atcgtgtttc cggaaggcac ccgcggttcc
     1921 ggggaggaga tcgctccctt caagagcggc atctaccacc tctgtcggga gaagcccgga
     1981 atcgagctcg tgcccgtata cctggacaac atgaaccgga tcctcccgaa gggcgagatg
     2041 ctcccggtcc cgatgctctc ccgcgtgatc ttcggccggc cgatgacgct cgagcccggc
     2101 gagaccaaac cggcatttct ctcgcgggcg cgggccgccg tgcaggagct ctccaacctg
     2161 tgacgggtct ttcggaccgc gcgctcgtgc ggctcgtggg cggcgtgctc gttctgctcg
     2221 tcgtcgccac cgccgtcggt ctcgcgctct cccggcgggc ccggacggag gaagcgcgcg
     2281 cgacgatcgc gaacctgaac gcgcgcatcc gcgcgtggtg ggcgatggtc gtgattttca
     2341 cgatcgccgt ctggaccggc ggaatcggct cgatcgttct cttcgcgttg atgtcgctac
     2401 tcgcattgcg cgagttcgtc acgctcgcgc cgacgcgccg cgaagaccac cggacgcttc
     2461 tctggagctt cttcgccgtc cttccgctcc agtactacct gatcgccatc cgctggtacg
     2521 ggctgttcgc gatcatgatc ccggtctacg ccttcgtgct cgtcgccgtg tcgaacgtga
     2581 tcgccggccg cacggaccgg ttcctcgagc gcaccgcgac gattcactgg gggctgatgg
     2641 tgtgcgtgta ctgcgtcagc cacgcgccgg cgctcctgat cctcgacatt cccggctacc
     2701 ggacgaacgc gaaactgctc gtctttctcg tgctcgtcgt ccagatctcc gacgtgctcc
     2761 agtacgtctt cggcaagctc ttcggaaagc atccgatcgc tccgtcgatc tcgccgaaca
     2821 agacgtggga aggattcgtg gggggagtcg caaccgcgtc cctcgtgggc acggcgatct
     2881 ggtgggcgac gccgttcacg ccgctcgtgg cgctcgcgat gtcggttgcg gtcacgctcg
     2941 ccggcttcgc cgggggtctc gtgatgtcgg cgatcaagcg cgaccggggc gtgaaggact
     3001 acggggcgct catccagggc catgggggga tgctcgaccg catcgactcg ctgtgcttcg
     3061 ccgcgccggt gttctttcac ctgacgcggt ttttcttcac gccttagtcc ggcggagcgg
     3121 cggtgcggtt cggcagggca cgggccgcga cccgacggcc ggccgtctaa gacgaagggc
     3181 ggacagacct ccgtacctcg atcttctgcc cgacttcgat caccatcaga tcctcaccga
     3241 gttccagagg ccccgcgtag gtcctgcgcg tcggttgaag caggctctgc tcggaggcgg
     3301 tcggtggaac gatgtgcgaa tacaccgcca gccggggttt ggcccgcgaa aacacttccg
     3361 ccgcctgctc gggggtcgtg tgataggcaa cgattctctt cgcgcgctct ggcgaaagac
     3421 ccgactgctg gagggcttct ggcgcgatca cctcgtggac caggacgtcc acccctcggg
     3481 cgtaccggat gaggttttcg gagaaacgcg tgtcaccgga gagcacgacg gagcggcccg
     3541 aataatccac gcggtatccg aaggccggct tgaccggcgg gtgatccacc tcgaacgccg
     3601 tgacctttgc cccaccgctc tcgtacacga cgccttcccg aatgtcctcg acctcgagca
     3661 cgaccccctc cggcgccgcg cggtcgttct gcagccgaac ctggatgtcg gactcgtaag
     3721 cccgctcgag gtgcgacatc atcttcctcg tcccgcgcgg accccacacg cgaagcggca
     3781 cactccgccc gggcaccacc agccaccccg tgagccacag atccggaaag ccgaccacgt
     3841 ggtcggagtg cagatgggtg aagaaaaccc ccttcacgtc ctgccatcgc acgttcgact
     3901 gcatgagacg ttgcagagcg ccgcgccctg catcgaagag gaacttctgc tctccggcct
     3961 c
//
LOCUS       DASWAV010000065         4141 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U2665
            k141_562939, whole genome shotgun sequence.
ACCESSION   DASWAV010000065 DASWAV010000000
VERSION     DASWAV010000065.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960316
            Sequence Read Archive: SRR10260007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 4141)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4141)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:47:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,929
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4141
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_562939"
                     /isolate="SMAG_U2665"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.36444 N 132.31889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            78..1679
                     /locus_tag="VF958_01440"
     CDS             78..1679
                     /locus_tag="VF958_01440"
                     /inference="COORDINATES: protein motif:HMM:NF025082.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEX9147800.1"
                     /translation="MQIPNKSDSFRVVAIVSAFNEADIISPVIEHLVQNGVDVYLIDN
                     HSTDGTAEEARRWLRKGLIGIEQFPADRPAGEGPPPFDWEAILRRKEELAKELSADWF
                     IHHDADEFREAPWPGMNLRDAIRWVDCLGYNCIDFRVLNFPPLGNGFEPGAPPERAFT
                     HWEDPVVYDTLQRKAWKAQKTPVSLAASGGHEARFPGRRVFPIRFLLRHYPIRSQEHG
                     MRKVFDERRKRFVEKERAKGWHVQYDSIGESHSFLRDPEGLHPYDPDRVRLDLMLENE
                     STREAEGRARELAAAAEAHQKTLEAAQRELAASLGEREAQVSNLKMELAEVRRRASGL
                     EHERETFAAHARETEIHSANLQSRIAELERHASGLENDRKTLAAHIAHLEAVRVGQDS
                     HVARLEELRAAREAEAASAAAKLAELERHAAGLEKDRETLAAHISHLDAVLHEETFAH
                     AELVGRNLENQSRAELLEARSRDLEDRFTTLRNQMNRVEGLRQEAEDRAARAERELTA
                     MRQSTFWRMTAPVRWALDQLKELFR"
     gene            1749..2204
                     /locus_tag="VF958_01445"
     CDS             1749..2204
                     /locus_tag="VF958_01445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9147801.1"
                     /translation="MRVRRLSPVTLVLVFLFVASLSATPAQKGKPAAAPPKPVPTVMG
                     WVDKATHAPGSGILDVAGWAADSRTGAPVARVEILLNERVVGLASTGEVRRDVVGIFK
                     RNDYAKAGWKARIDLKAVRPGKYRVTARAWNARGESAVLNTGPVDIQVP"
     gene            2233..2595
                     /locus_tag="VF958_01450"
     CDS             2233..2595
                     /locus_tag="VF958_01450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9147802.1"
                     /translation="MEKSRGGAIPPIEGVRRGDHPGAASETTTEDARSLLAARRQGEA
                     EAESLDLTLRLKESLTRERRLLESLEETREALEFVGQTERELRAQVERYATYHRAVER
                     SMAWRVVQFLRGLVGRRW"
     gene            2595..2885
                     /locus_tag="VF958_01455"
     CDS             2595..2885
                     /locus_tag="VF958_01455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9147803.1"
                     /translation="MDSAARSTIARLEARVAVLEKRLAERSAWIRAFARDAREEDLIR
                     LTRAVLALPPLPRPGFGLRGWSETTSLSSGEVEATMAELWRSLTPFELSDEE"
     gene            2918..>4141
                     /locus_tag="VF958_01460"
     CDS             2918..>4141
                     /locus_tag="VF958_01460"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX9147804.1"
                     /translation="MIDRKATLIVLAWNRWDLTRRCLDTLKQTDLTGAEVLVVDNGST
                     DETPEGLQGYDWIRVLRIPENLGFVRGNNAGIAAADASSDVVLLNNDLEFPQADWLQR
                     LRECAHSAPRVGIVGCRLKFPDGRLLHAGTYIMPDTMWGQQIGSLEKDIGQFSATRPV
                     EGIVFACAYIKRELLDAIGGLSEDFQSYFEDTDYCLRASEAGFSTLVCGDVTLVHHEH
                     GSTDGDPGEFEKIFLGSRETFRKKWHERLEKRYLREVLWQSIMNFPTGYAMSCREILR
                     ALDRVGVRAAYEYVYGPGTPFPVVEPEGNGDYVMNVIRGRTVKPDVAVVYGQGDVFLR
                     NRGKRRVGFTMLEVDGFPSDWVRQGNEMDEVWVPTRFNQEGFQNCGLKRPIHTIPLGV
                     DTNYFHSALKAYPNPM"
BASE COUNT          743 a         1333 c         1433 g          632 t
ORIGIN      
        1 cgcgaccaag aatagccgag cctgagtccg accgcggccc cgggagtgtc ggacatccga
       61 gctagacttg agagacattg cagattccga ataagagcga ttccttccgc gtcgtcgcga
      121 tcgtctcggc gttcaacgag gcggacatca tctcgcccgt catcgaacac ctcgtccaga
      181 acggggtcga cgtctacctg atcgacaacc actcgaccga cggcaccgcc gaggaggcgc
      241 ggcggtggct ccgcaagggg ctcatcggca tcgaacagtt tccggcggac cggccggcag
      301 gagagggtcc gccgccgttc gactgggaag cgatcctgcg ccgcaaggag gaactcgcga
      361 aagagctgtc ggcggactgg ttcatccacc acgacgccga cgagttccgc gaggcgccgt
      421 ggccggggat gaacctgcgg gacgcgatcc gctgggtaga ttgcctcggc tacaactgca
      481 tcgacttccg ggtcttgaac ttcccgccgc tcggcaacgg tttcgagccg ggcgctccgc
      541 cggagcgagc gttcacgcac tgggaggacc cggtcgtgta cgacaccctc cagcgaaagg
      601 cctggaaggc gcagaagact ccggtctcgc tcgccgcgtc gggcggccac gaggcccgct
      661 ttccggggcg ccgtgtgttt ccgatccgtt ttctcctccg ccactacccg attcggagcc
      721 aggagcacgg catgcggaag gtcttcgacg agcgcaggaa gcgcttcgtc gagaaggagc
      781 gcgccaaggg ctggcacgtg cagtacgact cgatcgggga gagccattcg ttcttgcgtg
      841 accctgaagg gctccatccg tacgatccgg accgcgtgcg tctcgacttg atgctcgaga
      901 acgagtcgac ccgggaggcc gaaggaagag cgcgcgagct cgccgccgcc gccgaggcgc
      961 accagaagac cctcgaagcg gcacaaagag agctcgccgc cagtctcggc gagcgcgagg
     1021 cgcaggtctc gaatctgaaa atggagctcg ccgaagtccg gcgtcgcgct tccggcctgg
     1081 agcacgagcg cgagaccttc gcagcgcacg cgagagagac cgagatccat tcggcgaacc
     1141 tccagagccg gattgcagag ctcgagcgtc acgcttccgg cctcgagaac gaccgcaaga
     1201 cgctcgcagc gcacattgcg cacctcgaag ccgtgcgagt cggccaggac agccacgtcg
     1261 cgaggctcga ggagctccgg gccgcgcggg aagccgaggc cgcgagcgcc gccgcgaagc
     1321 tggcggagct cgaacggcat gcggccggac tcgagaaaga tcgagagacg ctggctgctc
     1381 acatttccca tctcgacgcg gtgctgcacg aggagacgtt cgcgcacgcc gagctcgtgg
     1441 gccggaacct cgagaatcag tcgcgagcag agctgttgga ggcgaggtcg cgcgatctgg
     1501 aggatcgttt caccacgttg cgcaatcaaa tgaaccgcgt ggagggtctc aggcaggagg
     1561 cggaggaccg cgcggcgcgg gccgaacgcg agctcacggc gatgcggcag tcgacgttct
     1621 ggcggatgac ggcgccggtg cggtgggcac tcgatcaact gaaggagctc ttccggtaac
     1681 ccggtcgagg attgcgacgt tctgtccgag gtggtagttt cccttggatt ccattcagaa
     1741 agggcgaaat gagagtcaga cggctttccc ccgtgacgct ggtcctcgtg ttcctattcg
     1801 tggcctcgct ctcggcaact ccggcccaga aagggaagcc ggcggctgcg cccccgaagc
     1861 cggtgccgac agtgatgggg tgggtcgaca aggcgacgca cgcccccggg tcggggatcc
     1921 tcgacgtggc cggatgggcc gccgactccc ggacgggtgc tccggtcgcc agagtcgaga
     1981 tcctgctgaa cgaacgggtg gtcggcctcg cgagcaccgg cgaagtccgc cgcgacgtcg
     2041 tcggaatttt caagcgaaac gattacgcga aggcgggctg gaaggcgcga atcgacctga
     2101 aagccgtgcg ccctggcaag taccgcgtca cggcccgcgc gtggaacgcg cgcggcgagt
     2161 ccgcggtcct gaacacgggc cccgtcgaca tccaggtgcc gtagcggcgt gagcaccggg
     2221 cgggaggtcg gcgtggagaa atctcggggc ggcgcgatcc cgccgatcga gggcgttcgc
     2281 cgcggtgatc atcccggcgc cgcgagtgag acgacaaccg aggacgcccg gagtctcctc
     2341 gcggcccgcc ggcagggaga ggccgaggcg gaatcgctcg acctgactct ccgtctcaag
     2401 gagagcctga cccgggagcg acggctcctc gagagcctcg aggaaacgcg cgaggccctc
     2461 gagttcgtgg gccagacgga gcgcgaattg agagcccagg tcgaacgcta cgccacctat
     2521 catcgggcgg tcgagcgctc gatggcatgg cgggtcgtgc agttcctgag aggactcgtc
     2581 ggacggcggt ggtgatggat tcggctgccc ggtcgacgat cgcccgactc gaggctcggg
     2641 tcgcggtcct cgagaagcgg ctcgcggagc gttccgcctg gatccgagca ttcgcgcgcg
     2701 acgctcgcga ggaggatctg atccggctga cccgggcggt cctcgccctg ccccccctgc
     2761 cgcgacccgg cttcggcctg cgcggctgga gcgagacgac gtcgctctcg tcgggcgagg
     2821 tcgaggcgac gatggccgag ctctggcgct cgctcacgcc cttcgagctc tcggacgaag
     2881 agtagtccgg cgggctcgtc ccgccttcga acggaagatg atcgaccgga aagcaacgct
     2941 catcgtgctc gcctggaatc gctgggacct gacccggcgg tgcctcgaca cgttgaagca
     3001 gaccgacctc acgggagccg aagtcctcgt cgtggataac ggctcgaccg acgaaacgcc
     3061 cgaggggctc cagggctacg actggatccg cgtcctgcga atccccgaga acctcgggtt
     3121 cgtccgcggc aacaacgccg ggatcgcagc ggcggacgca tcctcggacg tcgtgctcct
     3181 gaacaacgac ttggagtttc cccaggccga ctggctccaa cggcttcgcg agtgcgctca
     3241 ctcggcgccg cgggtcggga tcgtcggatg ccggctgaag ttcccggacg gacgccttct
     3301 ccatgccggc acttacatca tgcccgacac gatgtgggga cagcagatcg ggtcgctcga
     3361 gaaggacatc ggccagttct cggcgacgcg accggtcgag gggatcgttt tcgcctgcgc
     3421 ctacatcaag cgcgagctgc tcgatgcgat cggcggtctc tcggaggatt tccagtcgta
     3481 cttcgaagac accgactact gcctgcgagc ctcggaagcc gggttttcca cgctcgtatg
     3541 cggcgacgtg accctcgtgc accacgagca cgggtcgacc gacggcgatc cgggggagtt
     3601 cgagaagatc tttctcggca gccgggaaac tttccggaag aagtggcacg agcggctcga
     3661 gaagcgctac ctacgcgaag tcctctggca gtcgatcatg aactttccga ccggatacgc
     3721 gatgagctgc cgggagatcc tgcgcgcact cgaccgcgtc ggcgtgcgcg ccgcctacga
     3781 gtacgtgtac ggccccggca cgcccttccc ggtcgtcgag ccggagggga acggcgacta
     3841 cgtgatgaac gtcatccgcg ggaggacggt gaagccggac gtcgcggtcg tttacggcca
     3901 gggggacgtc tttctccgca accggggaaa gcgccgcgtc ggcttcacga tgctcgaggt
     3961 ggacgggttt ccgagcgact gggtccggca gggcaacgag atggacgaag tctgggtgcc
     4021 gacccggttc aaccaggagg gttttcagaa ttgcggcctg aagcggccca tccacacgat
     4081 cccgctcgga gtcgacacga actacttcca ctctgcgctg aaggcctacc cgaacccgat
     4141 g
//
LOCUS       DASWAV010000066         5591 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U2665
            k141_484028, whole genome shotgun sequence.
ACCESSION   DASWAV010000066 DASWAV010000000
VERSION     DASWAV010000066.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960316
            Sequence Read Archive: SRR10260007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 5591)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5591)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:47:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,929
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5591
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_484028"
                     /isolate="SMAG_U2665"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.36444 N 132.31889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1058)
                     /locus_tag="VF958_01465"
     CDS             complement(<1..1058)
                     /locus_tag="VF958_01465"
                     /inference="COORDINATES: protein motif:HMM:NF012766.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M24 family metallopeptidase"
                     /protein_id="HEX9147805.1"
                     /translation="MRRLRATVALSFLTLLSESLPAADAPPAVPSLREQHRIRQEWLK
                     ARLERVLPALMRRHGVSMWIVENREYNEDPVFLSLVSPSLFAARRRTILVFYDRGPEK
                     GIERLALGGGSNGGLYTVYRDPDIENRELWGDAQWAMLRKIVDDRKPERIALDISHTH
                     AFSDGLSAGERELLEMALGPWVSRVVRAENLPLEYVEIRVSEMLPAYRDLMRISHGLI
                     ARAFSSEVITPGKTTTADVEWWLRQHVNDLGLGEWFAPTVDVQRKGLKAGAVVGEKSD
                     VVIERGDHLHVDFGIKGMGLATDTQHVGYVLREGETDAPAGLRKALENSNRLQDLVLE
                     RLKPGRSGNAILADVLAA"
     gene            complement(1055..2041)
                     /locus_tag="VF958_01470"
     CDS             complement(1055..2041)
                     /locus_tag="VF958_01470"
                     /inference="COORDINATES: protein motif:HMM:NF016228.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="complex I NDUFA9 subunit family protein"
                     /protein_id="HEX9147806.1"
                     /translation="MRVLVTGATGFVGTHLVNALLRRGHEVAVLARQPGAARNRYNRP
                     VEVYRGDVLDVASLARAFAGRDAVIHLVGIIHEEGAQTFDRMHREAVENVVASMKTAG
                     IRRLLHMSAMGTSPDSPSEYGRSKAAGEAAVRGSGLDWTIFRPSIIFGPGDGFVSLLA
                     PIVAKNPLFIPVIGKGETKFQPVSVYDVARIFADALENPETIGRAFEVGGPGVLTLNE
                     VYREIAAAIGKPRKPLLHLPLWWGKVLARVFESLARRGLIDAPPLTRDELKSLSRDNA
                     ADVSETVATFDGEWKEFRPGLQEYLTGGVKHDPRSGFGEEVDLEPVKVMRIR"
     gene            complement(2055..4079)
                     /gene="recG"
                     /locus_tag="VF958_01475"
     CDS             complement(2055..4079)
                     /gene="recG"
                     /locus_tag="VF958_01475"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:NF008168.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HEX9147807.1"
                     /translation="MPGALSLRDPVSRIPSIGAATAGRLSEAGFATVADFLLALPFRY
                     EDRRRFRKVSELVWGEPATLLVRFGAVRPFRMRRGTLRIEAVADDGTGAVRVVWHNRY
                     PSFTQAVQSGREAAIYGTPTTGPRGEMRIENPETEFFDEGQESDPLHSGRVVPIYHRA
                     AEIPSRRWRTLVRRTLDALSGKVAAAAASAAEILRAIENVHFPAEPPDAAEARRTLVD
                     EELLVLAARIEEKRARLRERAGIRLTADDALRRTAGRALPFPLTNAQKRAVREIAADL
                     GSGRAMARLLQGDVGSGKTVVAALALLLAARNRAQGAIMAPTEVLAEQHADTLSRLLA
                     GAGVRLALLTGRVRGAARQALLSALEAGQIDVLVGTHALVESPVKFRRLALVVVDEQH
                     RFGVAHRARLFRKGESPHVLVMTATPIPRSLAWAIYGELDVSVLDEKPPGRSPVTTRV
                     REEKDREGIYRFAAARVAAGERVYVVVPAIDEGERDVAATRETAERVAASAPGARVGV
                     LHGRLRPADRTRVLGDFTAGRIGILVSTTVVEVGVDVPEATFLVVENAESFGLAQLHQ
                     LRGRVGRSRRRSWCALVVGRNAGQDARERLDILEKTTDGFAIAEKDLETRGPGDLLGK
                     RQSGIPAMRVADPFRDLARLGDARRIVRERVEKGERIVSDLFGYPAASNA"
     gene            complement(4084..4869)
                     /locus_tag="VF958_01480"
     CDS             complement(4084..4869)
                     /locus_tag="VF958_01480"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HEX9147808.1"
                     /translation="MKAVFRTGPGTPKSPFERYLVTHPMGNIIFSEGEIGTEMFIIQS
                     GTVELLKDIGGETRVLSTLEKGDFFGEMSVLEDLPRTASARAKTDVELVRINGATFDA
                     MLKSNTEIAIRMMRKLSRRLREVTGVLEESLGRRVAEEEKPAEKRAMPQPNAFRLVAP
                     GGQPAFFLHTEGDTLIGRGDPVTGITPDVDLGPLDPQRSTSRRHAKIYPMAGKLYLME
                     EIGVMNGTFVNGSRVATGTPVPVSGGDELRFGLVPLTLQTAGA"
     gene            complement(4877..>5591)
                     /locus_tag="VF958_01485"
     CDS             complement(4877..>5591)
                     /locus_tag="VF958_01485"
                     /inference="COORDINATES: protein motif:HMM:NF036772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="LD-carboxypeptidase"
                     /protein_id="HEX9147809.1"
                     /translation="YRTLLRDPSVDAIFFARGGYGASRALGHLDPGEIRSYPKIHLGG
                     SDLTALFAFVAKHAALVACYGPMVAVQMAGEAELDWERVLSGERPETAVVASGDVVAS
                     GVGEGPLVGGCLSLVASLCGTPEAVDGRGSVLFWEDVGEETYRLDRMLTQLERSGTFD
                     GLQAMIVGSIAPGRREGAESPEEVRTWLAQRFAGAPFPVAAGFPAGHAADTRTLPFGT
                     RVRVDLERGEIEFLESAVA"
BASE COUNT          895 a         1949 c         1909 g          838 t
ORIGIN      
        1 atcgcggcaa gaacgtcggc gaggatcgcg ttgcccgacc ggcccggctt caatctctcg
       61 agaacgagat cctgcagccg gttcgagttc tcgagcgcct tgcggagtcc ggccggcgcg
      121 tcggtctccc cttcgcgcag gacgtatccc acgtgctggg tgtcagtggc cagccccatc
      181 cccttgatgc cgaagtcgac gtggaggtgg tcgccccgct cgatgacgac gtcgctcttt
      241 tctccaacga cggctcccgc cttcaggccc ttgcgctgga cgtccacggt cggggcgaac
      301 cactcaccga ggccgaggtc gttgacgtgc tgccgcagcc accactcgac gtccgccgtc
      361 gtcgtcttcc cgggcgtgat gacttcgctg gaaaacgcgc gcgcgatcag gccgtgcgaa
      421 atccgcatca ggtcgcgata cgcaggcagc atctcggaga cgcggatctc gacgtactcg
      481 agcgggaggt tctccgcgcg gacgacccgc gacacccacg gcccgagcgc catttcgagc
      541 agctcgcgct cccccgccga gagcccgtcg gagaacgcgt gcgtgtggga gatgtcgagc
      601 gcaattctct ccggcttgcg gtcgtcgacg attttgcgca gcatggccca ctgcgcatct
      661 ccccagagct cgcggttctc gatgtccggg tcccggtaca ctgtgtagag cccgccgttc
      721 gatccgccgc cgagcgcgag ccgctcgatc cccttctcgg gaccccggtc gtagaagacg
      781 agaatcgtgc gacgacgcgc cgcgaagagc gaaggcgaca cgagagagag gaagaccggg
      841 tcctcgttgt actcgcggtt ctcgacgatc cacatcgaga cgccgtgacg ccgcatcagc
      901 gcggggagca cgcgctcgag ccgcgccttc agccactcct gacggatgcg gtgctgctcg
      961 cggagcgagg gaaccgcggg cggtgcgtcg gcagccggaa gagattcgga gaggagcgtg
     1021 agaaacgaaa gggcgacggt cgcgcgcaat cgcctcatcg aatccgcatg accttgaccg
     1081 gttcgagatc gacctcttcg ccgaagccgg agcgggggtc gtgcttcact cctcccgtca
     1141 ggtactcctg gaggcccgga cggaattcct tccattcccc gtcgaacgtt gcgaccgtct
     1201 ccgagacgtc cgccgcgttg tcacgggaga ggctcttcaa ctcgtcgcgc gtcagcggcg
     1261 gcgcgtcgat caggccgcga cgcgcgagcg actcgaaaac gcgcgcgagc acctttcccc
     1321 accagagcgg aaggtggagg agcggcttgc ggggcttgcc gatcgccgcg gcgatctcgc
     1381 gatagacctc gttcaacgtc agaaccccgg gaccgccgac ctcgaacgcc cgtccgatcg
     1441 tctccgggtt ctcgagcgcg tcggcgaaga tccgcgcgac gtcgtagacc gagacgggct
     1501 ggaacttcgt ctcgcccttt ccgatcaccg ggatgaagag cggattcttc gcaacgatcg
     1561 gcgcgagcag ggaaacgaag ccgtcgccgg gaccgaagat gatggaagga cgaaagatcg
     1621 tccagtcgag gccggacccg cgcacggcag cctcccccgc ggccttcgat cgcccgtact
     1681 ccgacggaga gtccggcgac gtgcccatcg cgctcatgtg gaggagacgt cggatgccgg
     1741 ccgtcttcat cgaggcgacg acgttctcca ccgcctcgcg gtgcatccgg tcgaacgtct
     1801 gtgcgccctc ctcgtggatg atccccacga gatggatgac cgcgtctcgt cccgcgaagg
     1861 cgcgcgcgag tgaagccacg tcgagcacgt cgccacggta aacctcgacc gggcggttgt
     1921 agcggttgcg tgcggcgccg ggctgtcgcg cgagaacggc gacttcgtgc ccccgccgca
     1981 ggagtgcgtt gacgagatgc gttccgacga aaccggtcgc gccggtgacg agtactctca
     2041 tttcgctccg caggttacgc gttcgaagcg gcaggatatc cgaagaggtc gctcacgatt
     2101 ctctccccct tttcgacgcg ctcgcggacg attcgccgcg cgtcgcccag gcgagccagg
     2161 tcgcggaagg gatccgccac gcgcatcgcc ggaattcccg actgcctctt gccgagaaga
     2221 tctcccgggc cgcgggtctc caggtccttc tccgcgatcg caaagccgtc cgtcgtcttc
     2281 tcgaggatgt cgagccgctc gcgcgcgtcc tgtcccgcgt tgcggccgac gacgagcgcg
     2341 caccacgacc gtcgccgcga gcgcccgacg cgcccgcgga gctggtggag ttgcgcgagc
     2401 ccgaagctct ctgcgttctc caccacgaga aacgtggcct cgggaacgtc gacgccgacc
     2461 tcgacgaccg tcgtcgacac gagaataccg atccggcccg ccgtgaagtc gccgaggacg
     2521 cgcgtccggt ccgccggccg caggcggccg tggagcacgc ccacgcgcgc ccccggagca
     2581 gaggcggcga cgcgttccgc cgtctcgcgc gtcgccgcga cgtcgcgctc cccctcgtcg
     2641 atggcgggaa cgacgacgta gacccgctcg ccggcggcca cgcgcgcggc ggcgaaacgg
     2701 tagatcccct cacggtcctt ctcttcccgc acgcgcgtcg tcaccggcga gcgacccggc
     2761 ggcttctcgt cgagcacgga gacgtcgagt tcgccataga tggcccaggc gagcgatcgt
     2821 gggatcggcg tcgctgtcat cacgaggacg tgcggcgact cccccttccg aaacaggcgt
     2881 gcgcgatgag cgacgccgaa gcggtgctgc tcgtcgacga cgacgagagc gaggcgccgg
     2941 aacttgacgg gcgactcgac gagagcgtgg gttccgacga gaacgtcgat ctgtccggcc
     3001 tcgagcgccg agagcagcgc ctgccgcgcg gcgccacgga cgcggccggt cagaagcgcg
     3061 agccgaacac cggcgccggc gaggagccgc gagagtgtgt cggcgtgctg ctcggcgagt
     3121 acttcggtgg gggccatgat cgctccctgc gcgcggttcc gggcggcgag caggagagcc
     3181 agcgccgcga cgaccgtctt cccgctgcca acgtccccct ggagcagccg ggccatcgcg
     3241 cggccggagc ccagatccgc ggcgatctcg cgaaccgccc gcttctgcgc gttcgtgagc
     3301 gggaacggga gcgcccgccc tgccgtgcgc cgcagcgcgt cgtccgcggt cagacggatc
     3361 ccggcccgct cgcgcaggcg cgcacgcttc tcctcgatcc gcgcggcgag gacgagaagc
     3421 tcctcgtcga ccagggtccg acgcgcctct gccgcgtcgg gcggttcggc ggggaaatgg
     3481 acgttttcga tcgcgcgaag gatctcggcc gccgacgcgg ccgccgctgc gaccttcccg
     3541 gagagcgcgt cgagcgttcg ccggacgagg gtcctccagc ggcgcgacgg aatctcggcg
     3601 gcccggtggt agatcgggac gacgcgcccg gagtgcagcg ggtcgctttc ctgtccctcg
     3661 tcgaagaact cggtttcggg gttctcgatg cgcatctcgc cgcgtggacc ggtcgtgggg
     3721 gtgccataga tcgccgcctc ccgcccggac tgcaccgcct gggtgaacga cgggtagcgg
     3781 ttgtgccaga cgacacgcac cgcgccggtg ccgtcgtcgg cgaccgcctc gatgcggagc
     3841 gtcccgcgcc gcatgcgaaa gggacgaacg gccccgaagc gcacgaggag cgtcgccggc
     3901 tcaccccaga cgagctcgga gaccttccga aaccgccggc ggtcctcgta gcgaaacgga
     3961 agcgcgagga ggaaatcggc aacggtcgcg aagccggcct ccgaaaggcg tccagcggtg
     4021 gccgcgccga tggatggaat gcgggagacc gggtcgcgca gggagagcgc gccgggcagg
     4081 tgcctaggct ccggcggtct ggagagtgag cgggacgagc ccgaatcgaa gctcgtcgcc
     4141 ccccgagacg ggaacgggag ttccggtcgc cactcgggag ccgttcacga aggttccgtt
     4201 catcacgccg atctcctcca tcaggtagag cttaccggcc atcggataga tcttcgcgtg
     4261 ccggcgcgac gtcgagcgct gaggatcgag cggaccgagg tccacgtcgg gtgtgatccc
     4321 ggtaaccgga tcgccccgtc cgatcagcgt gtccccctcg gtgtgcagga agaacgccgg
     4381 ctgacctccg ggggcgacga gccggaacgc gttcggttgg ggcatggcgc gcttctccgc
     4441 cggcttctcc tcctccgcga cgcgccgccc gagcgactcc tcgagcaccc cggtgacttc
     4501 ccgcagccgg cgcgagagct tgcgcatcat ccgaatggcg atctcggtgt tgctcttcag
     4561 catcgcatcg aaggtggcgc cgttgatgcg gacgagctcg acgtccgttt tcgcgcgcgc
     4621 ggatgccgtc cggggcagat cttcgagcac gctcatctcc ccgaagaagt cgcccttctc
     4681 gagcgtcgac agcactcgtg tctcgccgcc gatgtccttc aggagctcga ccgtgcccga
     4741 ctggatgatg aacatctcgg tgccgatctc cccctcggag aaaatgatgt tccccatcgg
     4801 atgggtcacg aggtagcgct cgaaagggct cttcggagtg ccgggcccgg tgcggaagac
     4861 ggctttcacg ggcggctcac gcgaccgcgc tttcgagaaa ctcgatctcc cctcgttcga
     4921 ggtcgacccg gacacgtgtt ccgaatggaa gcgtgcgcgt atccgcggcg tgtcccgcgg
     4981 ggaaccccgc ggcgaccggg aacggagcgc cggcgaagcg ctgggcgagc cacgttcgga
     5041 cctcctccgg cgattccgcg ccctcgcgcc ggcccggcgc gatcgagccg acgatcatcg
     5101 cctgcagccc gtcgaatgta ccggagcgct ccaactgggt caacattcgg tccaggcggt
     5161 aggtctcttc tccgacgtcc tcccagaaga ggacggagcc gcgcccgtcg accgcctcgg
     5221 gcgtcccgca gagggacgca acgagcgaga gacagcctcc gacgagcgga ccttcaccca
     5281 cgccggacgc gacgacgtcg ccggacgcga cgacggcggt ttccggccgc tcccccgaaa
     5341 gaacgcgctc ccagtcgagc tccgcttcgc cggccatctg cacggcgacc atgggaccgt
     5401 agcaggcgac gagggcggcg tgcttcgcga cgaaggcgaa gagcgcggtc agatccgacc
     5461 cgccgaggtg aatcttcggg taggaacgaa tctcgccggg atcgaggtgc ccgagagcgc
     5521 gcgaggcccc ataccccccc cgcgcgaaga agatcgcgtc gacggacgga tcccgaagga
     5581 gagttctgta t
//
LOCUS       DASWAV010000067         5932 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U2665
            k141_643762, whole genome shotgun sequence.
ACCESSION   DASWAV010000067 DASWAV010000000
VERSION     DASWAV010000067.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960316
            Sequence Read Archive: SRR10260007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 5932)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5932)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:47:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,929
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5932
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_643762"
                     /isolate="SMAG_U2665"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.36444 N 132.31889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..396
                     /locus_tag="VF958_01490"
     CDS             <1..396
                     /locus_tag="VF958_01490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9147810.1"
                     /translation="AAHMTPEEVRSALERQIVVQNYLAERFRPIAVADEERAREEYEK
                     FYVPERRAANLAVPPFEAVTDEMRKRAQERAFEEEIGKWRRELRQKARVAIYRIPVPI
                     PTGRQRVELSSIAPVTPIPASTPATTPKP"
     gene            complement(400..738)
                     /locus_tag="VF958_01495"
     CDS             complement(400..738)
                     /locus_tag="VF958_01495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002849116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine triad nucleotide-binding protein"
                     /protein_id="HEX9147811.1"
                     /translation="MADCVFCKIVAGEIPSKRVYEDESVYAFEDIAPKAPTHVLVVPR
                     KHIARLADVTAGDEALLGALAARAAAIANERGLSDFRLVANNGEGAGQSVFHLHFHLL
                     GGRSMRWPPG"
     gene            complement(752..1999)
                     /gene="murA"
                     /locus_tag="VF958_01500"
     CDS             complement(752..1999)
                     /gene="murA"
                     /locus_tag="VF958_01500"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018433813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HEX9147812.1"
                     /translation="MDKFVIDGPTRLEGEVAISGAKNAALPCLAATLLTAERVRLTNL
                     PRVADVRTMQKVLSHIGARVEAGPETVNVAVPEIANADAPYDLVKTMRASVLVLGPLL
                     ARAGSVRVSLPGGCAIGVRPIDLHLAAFRKLGAEVSLQHGYVEAKASRLTAADIAFEH
                     VTVTGTENAMLAATLARGTTRLFNAAREPEVDDLAKLLVSMGARISGAGTEAITIEGV
                     DALHGAEHEIVPDRIEAGTYAIAAAATRGDVAVRRCRPEHLRALTSRLTAIGARVEAD
                     DGTLRVRAEGPLPAQDVATAPYPGFPTDLQAQWTALATSLDGVSTITETIFENRFHHV
                     AELIRMGAKIRLEGRWAVVEGCPLTGANVMASDLRASAALAIAALTAEGRTVIDRVYH
                     LDRGYVRMEEKLNALGASVERVS"
     gene            complement(2011..2853)
                     /gene="prmC"
                     /locus_tag="VF958_01505"
     CDS             complement(2011..2853)
                     /gene="prmC"
                     /locus_tag="VF958_01505"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: protein motif:HMM:TIGR00536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HEX9147813.1"
                     /translation="MTLAERLSDAASRARRAGSDATSWDARLLLAHAMGHDKPLALDL
                     RDDVPSPAASRFEDLWERRLSGVPVQHLVGEWDFYGRPFFVDSRALVPRPETELMIET
                     ALAEAPGARRALDLGTGSGVLAVTWILERPGSRAVAIDASTDALALARANAARHAVLE
                     RLDLVASDWTTALSREISFDLAISNPPYLTLGDAAELSVTVRDHDPALALFAGEDGLG
                     AIRRLLNDLPAHLASGAPFLFEIGAGQADAVTDEVAARPDWRLVRFAPDLAGISRAAV
                     LRRS"
     gene            complement(2850..3929)
                     /gene="prfA"
                     /locus_tag="VF958_01510"
     CDS             complement(2850..3929)
                     /gene="prfA"
                     /locus_tag="VF958_01510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011393874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016149 - translation release factor
                     activity, codon specific [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HEX9147814.1"
                     /translation="MFEKLEEIRRKYEEVRARLSDPDFVQDHRAVRDAQKTLAEFEPI
                     IAKNEEYRRVAAELDGTRELVESLSPGEELYQMAAAERAELTTRLERIEQELKLLLVP
                     RDPNDSRNVFIEIRAGTGGEEAALFAAQLFRMYQRYAEARRWKVEIVDRDDTGIGGIK
                     AITALVEGDGAYSRLKYESGVHRVQRVPETEASGRIHTSAATVAVLPEAEDVDVKVEE
                     KDLRVDRFCASGPGGQGVNTTYSAVRITHLPTGVVVQCQDERSQIKNKAKALRVLKAR
                     LLEMEREKQEGAIAAERKKMVGSGDRSEKIRTYNFPQSRITDHRIGFTTHRLVEVLDG
                     NLDELVEPLSAQFQAERLREEMARA"
     gene            complement(4047..4265)
                     /gene="rpmE"
                     /locus_tag="VF958_01515"
     CDS             complement(4047..4265)
                     /gene="rpmE"
                     /locus_tag="VF958_01515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015390726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L31"
                     /protein_id="HEX9147815.1"
                     /translation="MKKNLHPEYHEVLVHCACGETWTTGSTKKELRLEICSKCHPFFT
                     GKQKLVDSAGRVERFRKRYEKKASSAAS"
     gene            4377..>5932
                     /locus_tag="VF958_01520"
     CDS             4377..>5932
                     /locus_tag="VF958_01520"
                     /inference="COORDINATES: protein motif:HMM:NF018884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HEX9147816.1"
                     /translation="MFYVYVSAPGMAARKFPLEKPVTTIGRSSMNDLPISDKMLSRQH
                     ARIVKDDDGGMLVEDLGSRNGTFLNGERLAAPQPIKAGDRITLGGVTLKLESESTTRV
                     RIEAGGQDALDNTILKASAELLRAQHQETDPRLPAEQLSKLIESLRVVNELTIELLRD
                     VSVDELLKFLMDKVFETLMPDRGVVLLRSRVTNELIPAVVRVAEGMSAEDIRLSKTLV
                     NQVVEKRNGLILMDTSTGSGVSLADSIRLSGIKSVLAAPLENEGEVVGLIYVDSQVGH
                     RSFEEADLRLLTSLANVAAAKIQNARLMAEAAEKKQMDREFALAREIQQRLLPESPPT
                     IPGYELQGSNTASRQVSGDYFDFRARSDGKVYAAIADVCGKGVGPALLMASLQASFHA
                     WADESVAVSEMTGRLSEAISRRTGPDRFITFILVLLDPATGEIEYTNAGHNPGLLLRK
                     DGSLQELASHGLPLALFPGRPYGSSRFTLAPGDLLCLYTDGVTEANSPDGTEFGFPRL
                     KDFFGKQVGQA"
BASE COUNT          935 a         2029 c         2028 g          940 t
ORIGIN      
        1 gccgcgcaca tgacgcccga ggaagtccgc tccgcgctcg aacgccagat cgtggtccag
       61 aactacctcg ccgagcgctt ccggccgatc gccgtcgccg acgaggaacg cgcgcgggag
      121 gagtacgaga agttctacgt cccggagcgg cgcgcggcga acctcgccgt tcctccgttc
      181 gaggcggtca cggacgagat gcgcaagcgc gcgcaggagc gtgcgttcga ggaagagatc
      241 gggaagtggc gccgcgagct acggcagaag gcgcgcgtcg cgatctaccg gatccccgtt
      301 ccgattccga ccgggcgaca gcgggtcgag ctctcttcca tcgcgccggt cacgcccatc
      361 cccgcctcga ccccggcgac cactccgaaa ccctgagtct taccccggcg gccatctcat
      421 cgagcggcct ccgaggaggt ggaagtggag gtggaagacc gactgaccgg cgccttcgcc
      481 gttgttcgcg acgagccgga agtccgagag cccgcgctcg ttcgcgatcg ccgcggcccg
      541 cgccgcgaga gctcccagca gggcctcgtc gccggcggtg acgtcggcca ggcgcgcgat
      601 gtgcttccgg gggacgacga gcacgtgcgt gggcgctttg ggcgcgatgt cctcgaacgc
      661 gtagacactc tcgtcctcgt agaccctctt cgagggaatc tcgcccgcga cgatcttgca
      721 gaagacgcag tctgccacgc cgtccccctg gtcaggaaac gcgctcgacc gaggcaccga
      781 gagcgttcag cttctcctcc atccgcacgt aacctcggtc gagatggtat acgcggtcga
      841 tcaccgtacg cccctccgcg gtcaaggccg cgatcgcgag cgccgcggat gcccgcaggt
      901 cgcttgccat cacgttcgcg ccggtcagag gacatccctc gacgaccgcc cagcgcccct
      961 ccagccggat cttcgcgccc atgcggatca gctcggcgac gtggtggaag cggttctcga
     1021 agatcgtctc ggtgatcgtc gagacgccgt cgaggctcgt cgcgagggcg gtccactgtg
     1081 cctgaagatc ggtggggaat ccggggtagg gagcggtcgc cacgtcctgg gccgggagcg
     1141 gaccttccgc ccgaacgcga agcgttccgt cgtccgcctc gactcgcgcc ccgatggccg
     1201 tcagtcggct cgtcagggcg cgcaggtgct ccggccggca gcggcgcacg gcgacgtcgc
     1261 cccgggtcgc cgcggcggcg atcgcgtatg ttcccgcctc gatgcggtcg ggcacgatct
     1321 cgtgctcagc cccgtgaagc gcgtcgaccc cttcgatcgt gatcgcttcc gtgccggcgc
     1381 cggagattct ggcgcccatc gagacgagca acttcgcaag atcgtcgacc tcgggctcgc
     1441 gcgcggcgtt gaagagcctc gtcgttccgc gagcgagcgt cgcggcgagc atggcattct
     1501 cggttccggt cacggtgacg tgctcgaagg cgatgtcggc ggcggtcagg cggctcgctt
     1561 tcgcctccac gtaaccgtgc tggagcgaca cctccgcccc gagcttgcgg aacgccgcca
     1621 ggtgcaggtc gatcggccgg acgccgatcg cgcagccgcc ggggagggag acccggacgg
     1681 accccgcgcg cgcgagcaat ggcccgagga cgagcacgga cgcgcgcatc gtcttgacga
     1741 gatcgtaggg cgcatcggcg ttcgcgatct cgggaaccgc gacgttcacc gtctcgggac
     1801 ccgcctcgac gcgcgcgccg atgtgagaaa ggactttctg catcgtgcgg acatcggcga
     1861 cccgggggag gttggtcagg cggactcgtt cggccgtgag cagcgtcgcc gcgaggcagg
     1921 ggagcgcggc gttcttggcc cccgagatcg cgacctcgcc ctcgagccgg gtaggaccat
     1981 cgatgacgaa cttgtccacg gcctcggggt ctaactgcgc cgaaggaccg cggcgcgcga
     2041 aattccggcg aggtccggag cgaaccgaac gagtcgccag tcgggacggg ccgcgacttc
     2101 gtcggtgacc gcgtccgcct gaccggcgcc gatctcgaaa aggaacggag ctccggaggc
     2161 gaggtgcgcg gggaggtcgt tcagcagccg tcgaatcgcg ccgagcccgt cctcgcccgc
     2221 gaagagcgcc aaggccggat cgtggtcgcg caccgtgacc gagagttcgg cggcgtctcc
     2281 gagcgtcagg tacggcgggt tcgatatcgc gagatcgaag gaaatctcgc gcgacagggc
     2341 cgtcgtccag tcgctcgcga cgagatcgag gcgctcgaga accgcgtgtc gcgcggcgtt
     2401 cgcccgcgcg agcgcgagcg catctgtcga agcgtcgatc gccacggcgc gggagcccgg
     2461 ccgctcgagg atccacgtca ccgcgagaac gccgctcccg gttccgaggt cgagtgcacg
     2521 ccgtgctccg ggcgcttcgg cgagagcggt ttcgatcatg agctcggttt ccggccgcgg
     2581 cacgagcgcg cgggagtcga cgaagaacgg tcgcccgtag aagtcccact cgccgacgag
     2641 gtgctggacg ggcacgcccg agaggcgtcg ctcccaaagg tcctcgaagc gcgaggcggc
     2701 gggggaggga acgtcgtccc ggagatcgag cgccaggggt ttgtcgtgac ccatcgcgtg
     2761 cgcgaggagc aaacgcgcgt cccacgaggt cgcgtccgac ccggcgcgcc gcgctcgcga
     2821 cgcggcgtcg gagagccgct cggcgagggt cacgcccgcg ccatctcttc gcggaggcgt
     2881 tcggcctgga actgcgccga cagcggctcg acgagctcgt cgagattgcc gtcgagaact
     2941 tccacgagcc gatgagtcgt gaagccgatg cggtggtcgg tgatccgcga ttgcgggaag
     3001 ttgtaggtgc ggatcttctc ggagcggtcg ccgctgccca ccatcttctt gcgttccgcc
     3061 gcgatcgcgc cctcctgctt ctcgcgctcc atctcgagca aacgcgcttt cagcacccgc
     3121 agcgctttcg ccttgttctt gatttgactc ctctcgtcct ggcactggac gaccacgccc
     3181 gtcggcaggt gcgtgatccg gaccgccgaa taggtcgtgt tgacgccctg tccccccggc
     3241 cccgacgcgc agaaacggtc cacccgcagg tccttctcct cgaccttgac gtccacgtcc
     3301 tccgcctccg gcaggacggc gacggtcgca gccgaggtgt gaatccggcc ggacgcctcg
     3361 gtctccggga cgcgctgcac gcggtggacg ccgctctcgt acttcaggcg cgagtacgcg
     3421 ccgtccccct cgaccagggc ggtgatggcc ttgatcccac cgatgccggt gtcgtcccga
     3481 tcgacgatct ccaccttcca tcgccgcgct tcggcgtagc gctgatacat ccggaagagc
     3541 tgcgccgcga agagagcggc ctcctcgccg ccggtccccg cgcggatctc gatgaagacg
     3601 tttctcgagt cgttggggtc ccgcgggacg aggaggagct tcagctcctg ctcgatccgc
     3661 tcgagccgcg tggtgagctc ggcgcgctcc gcggccgcca tctgatagag ctcctctccg
     3721 ggggagagcg actccacgag ctcacgggtc ccgtcgagct cggccgcgac ccggcggtac
     3781 tcctcgttct tcgcgatgat cggctcgaac tcggcgagcg tcttctgcgc gtcgcgcacg
     3841 gcgcgatgat cctggacaaa gtccgggtcg gagagccgcg cgcggacctc ctcgtatttc
     3901 cggcggattt cttcgagctt ttcgaacatg ggtgttctcg attccggtcg tcagtttcga
     3961 gcggcagccg ggacggcaga ccgcggccgt gccgctagag ggcggaaaag ggaggtgcga
     4021 gcgggaggat ccgcgccgtc acggatctaa gacgcggcgc tcgaggcttt tttctcgtac
     4081 cgcttgcgga aacgctcgac ccggccggcg gagtccacca gcttctgctt tcccgtgaag
     4141 aacgggtggc atttgctgca gatctccagc cgtagctcct tcttcgtgga accggtcgtc
     4201 caggtctcgc cgcacgcgca gtggacgaga acctcgtgat actcggggtg caggttcttc
     4261 ttcatggaac cgcccattct aggagaggag gagcgagcgc gcaagcccgc gcccgcggag
     4321 ccggttaacg gctcacagct cggggcttac gaaccgggta gaatcgccgg accaacgtgt
     4381 tttacgtcta cgtgagtgcg cccgggatgg cggcgcgcaa gttcccgctc gagaagccgg
     4441 tcaccacgat cggccgctcc tcgatgaacg acctcccgat ctccgacaag atgctgtcgc
     4501 gccagcacgc ccgcatcgtc aaggacgacg acggagggat gctcgtcgag gacctcgggt
     4561 cgagaaacgg taccttcctg aacggggagc ggctcgcagc cccgcaaccc atcaaggcgg
     4621 gagaccgcat cacgctcggc ggcgtcacgc tgaagctcga gtcggaatcg acgacccgcg
     4681 tccggatcga agcgggcggc caggacgcgc tcgacaacac gatcctgaag gcttccgcgg
     4741 agctcctgcg cgcgcagcac caggagaccg atccacgcct gccggccgag cagctgtcga
     4801 agctgatcga gtcgctgcgc gtcgtcaacg agctcacgat cgagctcctc cgcgacgtct
     4861 cggtcgacga gctcctgaaa ttcctcatgg acaaggtatt cgagacgctc atgccggacc
     4921 ggggcgtcgt gctcctgcgc tcgcgcgtga ccaacgagct gattcccgcc gtcgtgcgcg
     4981 tcgccgaagg gatgtcggcc gaagacatcc gcctttcgaa gaccctcgtc aaccaggtcg
     5041 tcgagaagag aaacggcttg atcctcatgg acacgtcgac gggctcggga gtgtcgctcg
     5101 ccgattcgat ccggttgtcc ggcatcaaat ccgtcctggc cgcgcccctc gagaacgagg
     5161 gggaagtcgt cggcctgatc tacgtcgaca gccaggtcgg ccaccgctcg ttcgaggagg
     5221 cggacctgcg gctgctgacg tcgctggcga acgtcgcggc cgccaagatc cagaacgcgc
     5281 gcctgatggc ggaggccgcc gagaagaagc agatggaccg ggagttcgcg ctcgcgcggg
     5341 agatccagca gcgtctcctg cccgagagcc cgccgacgat ccccggatac gagcttcaag
     5401 gctccaacac ggcgtcgcgc caggtctccg gcgactactt cgacttccgc gcgcggtcgg
     5461 acggaaaagt ctacgcagcc atcgccgacg tctgcgggaa gggggtcggg ccggcgctcc
     5521 tcatggcgtc gctccaggcg tcgttccacg cgtgggcgga tgagtcggtg gcggtgtccg
     5581 agatgaccgg acggctctcg gaggcgatct cgcggcgcac gggaccggac cgcttcatca
     5641 cgttcatcct cgtgctgctc gatccggcga cgggggagat cgaatacacc aacgccggcc
     5701 acaacccggg gctcctgctc cggaaggacg gctcgctcca agagctcgct tcccacggcc
     5761 tgccgctcgc gctcttcccc ggccggccgt acggctcgtc gaggttcacc ctggcgccgg
     5821 gggacctcct ctgcctgtac acggacggcg ttaccgaggc gaacagcccg gacggaacgg
     5881 agttcggttt cccgaggctg aaggactttt ttggaaagca ggtcggtcag gc
//
LOCUS       DASWAV010000068         4959 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U2665
            k141_563365, whole genome shotgun sequence.
ACCESSION   DASWAV010000068 DASWAV010000000
VERSION     DASWAV010000068.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960316
            Sequence Read Archive: SRR10260007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 4959)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4959)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:47:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,929
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4959
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_563365"
                     /isolate="SMAG_U2665"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.36444 N 132.31889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..479
                     /locus_tag="VF958_01525"
     CDS             <1..479
                     /locus_tag="VF958_01525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9147817.1"
                     /translation="FPGGAYYPLTFRAGFQSKSRTVAYATSVVVEEIKRIASEPVTAE
                     ELNTAKKSFVETFPRTFATKAQVAITFAQDELTGRYASDPDYWKNYRAKIDRVTAADV
                     QRVAKKFLDSDRLVILIVGKKSDILAGDLKHKVELQTLAGNRLVDLPLRDPMTMKP"
     gene            481..690
                     /locus_tag="VF958_01530"
     CDS             481..690
                     /locus_tag="VF958_01530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9147818.1"
                     /translation="MAPPVPPVAVSRLLLLALVAAGAVISVAASPPLSSFAGGFAAGA
                     GAVIVLSFLSHPRREPSPSDEGKAS"
     gene            687..1382
                     /locus_tag="VF958_01535"
     CDS             687..1382
                     /locus_tag="VF958_01535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009850066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX9147819.1"
                     /translation="MRVLVIEDEEKIAQFLRKGLSEKGYSVEAVPDADAALERLESGP
                     YDLLILDLLLPGSRDGLELCREIRARGIPSKILMLTARDTVENKIEGLDAGADDYLVK
                     PFSFRELLARLRALSRRTEVSEPGPLSHGDLTYDPESREVRRRGEVIHLTAREGALFE
                     LLLRRRGKVVSRSEIQARIWEDSFDLSTNIIDVYINALRRKLDAGEKEKLIQTVRGVG
                     YRLGEPGETAPSA"
     gene            1385..2452
                     /locus_tag="VF958_01540"
     CDS             1385..2452
                     /locus_tag="VF958_01540"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX9147820.1"
                     /translation="MPRGRTTSWSLAAALLLGAAVIVLALSFAVSSSAVLIPVAAGLG
                     ALAVLVFLGGRLASPPREAVPLFTDRRSDPPFVSPATLELEKLRDLLEQTEGKPLKRI
                     PAGDLSGTALEMVERLNARFDALSGSEKEQQQFIADVSHELRTPLTVLRGTLEVALEE
                     ERPAEEYREVIGNALLEIRHLARISQNILFLARGESGRVTLSFSNQDLARFVADVTRE
                     LQPAAADHDLELTVEVPERAVIAFVDPGRMQQVLHNLLENSMRYTPRGGSVHVRLSTA
                     PEEARIEISDSGVGIPEADLPFVFERFFRSDRARRAYTGGSGLGLSIVRWIVEAHKGR
                     VEISSQPDKGTTVAVRLPLLT"
     gene            2449..2901
                     /locus_tag="VF958_01545"
     CDS             2449..2901
                     /locus_tag="VF958_01545"
                     /inference="COORDINATES: protein motif:HMM:NF024966.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HEX9147821.1"
                     /translation="MKGGTAVDAHQEVLDTINDLFVGTDDRDWDRVRAALAPQVLLDM
                     KSLTGAEPATISAREIIAGWEEGLRPLKAVHHQTGNFRIRVDDDGADAFCYGIALHYR
                     PNASGRNTRTFVGSYDFHLQRNGGRWRIDLFRFNMKYVDGNLELEKGS"
     gene            2912..4105
                     /locus_tag="VF958_01550"
     CDS             2912..4105
                     /locus_tag="VF958_01550"
                     /EC_number="2.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002986460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HEX9147822.1"
                     /translation="MRPVFVLSAARTPIGRFGGAFASLSAADLGVVAAAAAVERSGLP
                     PAAIDETIFGHARQAGGGPNTARQVSHRAGIPDSVPAYTVNKACASSLKALTLGALSI
                     AAGENEAVLVGGTECMSATPYLLTGARFGYRMGHSELVDGMYRDGFLCPLCGQLMGET
                     AENLVREYGILRAEQDAFATESQRRAAQAIASGRFADEIAPVTVTGKKGKTVVEGDEH
                     PRPDTTVESLAKLPTVFDPESGTVHAGNSSGITDGAAALVLASEEEAARAGVAPLARV
                     VAWRSAGVEPERMGVGPVPALRALLESEKLRLPDIDLLELNEAFAAQVIACARELPFD
                     LSRVNVNGGAIALGHPIGATGARIATTLLHEMKRRGAKRGVATLCVSGGMGMAVLFAR
                     DFASP"
     gene            4115..4801
                     /locus_tag="VF958_01555"
     CDS             4115..4801
                     /locus_tag="VF958_01555"
                     /inference="COORDINATES: protein motif:HMM:NF019342.4"
                     /note="Members of this family of hydrolases with an active
                     site Cys residue belong to MEROPS family C26; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HEX9147823.1"
                     /translation="MRIALTLDRDPTENNDYIRALLEAGARREEIVVLRPGDPIEGPF
                     DGVVIGGGDDVDPELYGREVLRDGKVKVDPARDSIDLAVFEEAWRGAVPTLGVCRGLQ
                     VMNVARGGTLFQHLPLESPSQIEHQRPKEANRRDHVVTVRPGTRLSGIAASGELDVNS
                     RHHQAVERPAEDFVVTATAPDGVIEALEARDGRWLVAVQWHPENLHDDPASKRLFRDF
                     VEEVRKRSSA"
BASE COUNT          780 a         1700 c         1674 g          805 t
ORIGIN      
        1 tgttcccggg cggcgcgtac tatccgctga ccttccgcgc ggggttccag tcgaagtcgc
       61 gcacggtggc ttacgcgacc tcggtcgtcg tggaggagat caagaggatc gcgtccgagc
      121 cggtcacggc ggaagagctg aacaccgcga agaagtcgtt cgtcgagacg ttcccgcgga
      181 cgttcgcgac gaaggcgcag gtcgcgatca cgttcgcgca ggacgagctg accggccgct
      241 acgcgagcga ccccgactac tggaagaact accgggcaaa gatcgacagg gtgacggcgg
      301 ccgacgtcca gcgtgtcgcg aagaaattcc tcgactccga caggttggtg atcctgatcg
      361 tcgggaagaa gtcggacatc ctcgccggcg atctcaagca caaagtcgag ctgcagacgc
      421 tcgcgggcaa ccggctcgtg gatctgcccc tccgcgatcc gatgacgatg aagccctgag
      481 atggcgccgc cggttccccc cgtcgcggtc tcgcggctgc tcctgctcgc gctcgtcgcg
      541 gcgggcgcgg tcatttcggt cgcggcatct ccgcctctct cctcgttcgc ggggggattc
      601 gcggcggggg ccggcgctgt catcgtgctg tcgtttctga gccacccgcg ccgcgaaccc
      661 tcgccttccg acgaagggaa ggcctcgtga gggttctcgt catcgaggac gaggagaaga
      721 tcgctcagtt ccttcggaag ggcctgtcgg agaagggata ctcggtcgag gcggtcccgg
      781 acgccgatgc ggcgctcgag cggctcgagt cgggtcctta cgatcttctg attctcgacc
      841 tgctgctccc gggctcgcgc gacggcctcg agctctgccg cgagatccgg gcgcgcggga
      901 ttccctcgaa gatcctgatg ttgaccgcgc gcgacacggt ggagaacaag atcgagggtc
      961 tggacgcggg cgcggacgac tacctcgtga agccgttctc gttccgcgag ctgctcgcgc
     1021 gtcttcgcgc gctctcacgc cgcaccgagg tctccgagcc ggggccgctg tcccatggcg
     1081 acctgaccta cgaccccgag tcgcgcgagg tccgccgccg cggcgaggtc atccacctga
     1141 ccgcccgcga gggcgctctc ttcgagctcc tgctgcggcg gcgagggaag gtcgtctccc
     1201 gctccgagat ccaggcacgg atctgggagg actccttcga cctctcgacg aacatcatcg
     1261 acgtctacat caacgccctg cgccgcaagc tcgacgccgg tgagaaggag aagctcatcc
     1321 agacggtccg cggcgtcggt taccgactcg gcgaaccggg cgaaaccgcc ccgagcgcat
     1381 agccgtgccc cgcgggcgaa cgacgagctg gtcgctcgcg gccgccctgc tcctgggagc
     1441 cgccgtgatc gtgctcgccc tctcgttcgc ggtctcctcg tccgccgttc tgatccccgt
     1501 cgcggccggg ctcggcgccc tggcagtcct ggtctttctc ggtggccggc tcgcgtcgcc
     1561 accgcgggag gcggtccccc tctttaccga tcggcgttcg gatccaccgt tcgtcagtcc
     1621 agcgacactc gagctggaga agctgcgcga ccttctcgaa cagaccgaag gcaagccgct
     1681 caagcgaatc ccggcgggcg atctgtcggg cacggcgctg gagatggtgg agcgcctgaa
     1741 tgcccgcttc gacgcgctct cgggatcgga aaaggagcag cagcagttca tcgcggacgt
     1801 ctcgcacgag ctgcgcacgc cgctcacggt gctccgcggt actctcgagg tcgcgctcga
     1861 agaggaacgg ccagccgagg agtaccggga agtgatcggc aacgcgctcc tcgaaatccg
     1921 ccacctcgca cggatctcgc agaacatcct ctttctcgcg cgcggggaaa gtggccgcgt
     1981 cacgctttcg ttttcgaacc aggacctggc gcgcttcgtc gcggacgtga cacgcgagct
     2041 ccaaccggcg gcagcggacc acgacctcga gctcaccgtg gaggtcccgg agcgcgcagt
     2101 gatcgccttc gtcgatccgg gccggatgca gcaggtcctc cacaaccttc tggagaactc
     2161 gatgcgctac acccctcggg gcggctcggt gcacgtccgg ctctcgaccg ctccggagga
     2221 ggcgcgcatc gagatctcgg actccggcgt cgggattccc gaggcggacc tgccgttcgt
     2281 tttcgagcgc ttcttccgca gtgaccgcgc gcgccgcgcg tacacgggag gctccggtct
     2341 cgggctctcg atcgtgaggt ggatcgtcga agcgcacaag ggtcgggtcg aaatttcgag
     2401 ccaacccgac aagggaacga cggtcgcggt ccgtctgccg ctcctcacgt gaaggggggt
     2461 accgcggtgg atgcgcacca ggaggttctc gacacgatca acgatctctt cgtcggcacc
     2521 gacgaccggg actgggaccg cgtccgcgcg gcgctcgctc cgcaggtgct cttagacatg
     2581 aagtcgctca ctggcgccga gccggcgacg atttctgcca gagagatcat cgccggatgg
     2641 gaagaggggc ttcggcccct gaaggccgtt caccaccaga cgggaaactt ccgcatccgc
     2701 gtcgacgacg acggcgcgga cgcattctgc tacggcatcg cgctccacta ccgcccgaac
     2761 gcatccggcc ggaacacgcg tacgttcgtc gggagctacg actttcacct tcagcgcaat
     2821 ggcggacgct ggcggatcga cctcttccgc ttcaacatga agtacgtcga cgggaatctc
     2881 gagctggaga aggggagttg agtatccgag catgaggccc gtattcgtcc tctccgcggc
     2941 ccgcacgccg atcggccggt tcgggggcgc cttcgcctcc ctctccgccg ctgacctcgg
     3001 ggtggtcgcc gcggccgccg cggtcgagcg ctcgggactc cctcccgccg cgatcgacga
     3061 aacgatcttc ggtcatgcgc ggcaggcggg cggtggcccg aacacggcgc ggcaggtctc
     3121 ccatcgcgcc gggattcccg actccgtgcc ggcgtacacc gtcaacaagg cgtgcgcctc
     3181 ttcgctgaaa gcgctcacgc tcggagcgct gtcgatcgcg gcgggcgaga acgaagcggt
     3241 tctcgtcggg ggaaccgagt gcatgtccgc aacgccctac ctcctcaccg gggcccgctt
     3301 cgggtaccgg atgggacact cggagctcgt cgacgggatg taccgcgacg gctttctctg
     3361 cccgctctgc ggccagctga tgggcgagac cgcggagaac ctcgtgcgcg agtacgggat
     3421 cctgcgggcg gagcaggacg ccttcgcgac ggagtcccag cggcgcgcgg cgcaggcgat
     3481 cgcctccggc cgatttgccg acgagatcgc gcccgtgacg gtcacgggaa agaaaggcaa
     3541 gaccgtcgtc gagggggacg agcacccgcg tcccgacacg accgtggaat ctctcgcgaa
     3601 gcttccaacg gtcttcgatc cggagtccgg caccgtgcac gcggggaact cctccggcat
     3661 caccgacggc gcggcggcac tcgtgctcgc ctcggaggaa gaagcggctc gcgccggcgt
     3721 cgcgcccctc gcgcgcgtcg ttgcctggag gtccgccggg gtcgagccgg agaggatggg
     3781 ggtcgggccc gttccggccc tccgcgcgct gctcgagagc gagaagctgc ggctcccgga
     3841 catcgacctg ctcgagttga acgaggcgtt cgccgcccag gtgatcgcct gtgcgcgcga
     3901 gcttccattc gatctttcgc gcgtcaacgt caacggcggc gcgattgctc tggggcatcc
     3961 gatcggcgcc accggcgccc gcatcgcgac gacgctcctg cacgagatga aaaggcgcgg
     4021 cgcgaagcgc ggcgtcgcga cgctctgcgt gtcgggcggc atggggatgg ccgtgctctt
     4081 cgcgcgcgat ttcgcttctc cttaggatcc gcacgtgcgt atcgccctca cgctcgacag
     4141 agacccgacc gagaacaacg actacatccg tgcgctgctc gaagcgggcg cgcggcggga
     4201 ggagatcgtg gttctgcggc ccggcgatcc gatcgaagga ccgttcgacg gagtcgtgat
     4261 cggcggggga gacgacgtcg atccggagct ttacggccgc gaggttctgc gcgacggaaa
     4321 ggtgaaggtc gacccggccc gcgactcgat cgatcttgcc gtcttcgagg aggcgtggcg
     4381 cggggccgta cccaccctgg gagtctgccg cgggttgcag gtcatgaacg tcgcgcgcgg
     4441 cgggacgctc ttccagcatc tcccgctcga gagcccgtcg cagatcgagc accaacgtcc
     4501 gaaagaggcg aaccgccgcg accacgtcgt caccgtccgg ccggggacgc gcctgtccgg
     4561 gattgccgcc tccggggagc tcgacgtgaa cagccggcac caccaggcgg tcgagcggcc
     4621 ggccgaggac ttcgtcgtca ccgcgacggc ccccgacgga gtgatcgaag ctctcgaagc
     4681 gcgggacggc cgctggctcg tcgccgttca gtggcatccc gagaatctgc acgacgatcc
     4741 cgcctcgaag cggctctttc gcgacttcgt ggaagaggtt cgaaagcgca gttccgcata
     4801 gcttgcagga attccacccc ggaaggccgg tgttgtccgg ccgtcggagc ctcaacgtca
     4861 gcaaggagcc gttcgcatga gacctcgcct cggaatcctc gtcctcgtcc tcgccatcat
     4921 tgctcttccc gccctcgccc agtccacgat gggagtcca
//
LOCUS       DASWAV010000069         5719 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U2665
            k141_723933, whole genome shotgun sequence.
ACCESSION   DASWAV010000069 DASWAV010000000
VERSION     DASWAV010000069.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960316
            Sequence Read Archive: SRR10260007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 5719)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5719)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:47:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,929
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5719
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_723933"
                     /isolate="SMAG_U2665"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.36444 N 132.31889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..202)
                     /locus_tag="VF958_01560"
     CDS             complement(<1..202)
                     /locus_tag="VF958_01560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9147824.1"
                     /translation="MRLRPTSLAAILVALATQLAMLAPLVPAAAGERCAMSCCAAAGE
                     GASCPMHAAPHGSSFRSCPSDPD"
     gene            184..1743
                     /locus_tag="VF958_01565"
     CDS             184..1743
                     /locus_tag="VF958_01565"
                     /inference="COORDINATES: protein motif:HMM:NF019607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HEX9147825.1"
                     /translation="MSGGVARFGGLYVPGPALRLREATLRKLAASLALVGLGVACRAP
                     AQSAPEIALSTVVTGLDRPTAIAHEGDSRLFLTLQRGRTVIVEEGRILPAAFLDIQSL
                     VSCCGEQGLLGLAFHPRYAENGFFFVDYTNTSGNTVIARYRVSPSNPNAGDAASGVVL
                     LTIPQPFSNHNGGQLAFGPDGYLYIGMGDGGSANDPMCNGQRDDSLLGKILRIDVDAN
                     VDTPPYYGIPPDNPFSAPGGARDEIWAKGLRNPWRFSFDRQTGDLYIGDVGQGAREEV
                     DFQAAGSAGGQNYGWKVMEGTACGGGGTSGCPSSTAACNSPGLTPPILEYPHSGGDCS
                     ITGGHVYRGPFFPELAGTYLYGDYCTGKIWGATRGPTGTWSARLFSAHAAGLTTFGEG
                     AAGEIYLATESGLLARIVNANPVAPTIDSVEPASGSTRGGESVIVTGGSFVLGVTVSF
                     EGVTAPSTVILDPNGTRLLVVTPPHAAGPVDVVVRNPDGKSAALTSGYGYAAPSRVAP
                     PPRIPRTVTRP"
     gene            complement(1808..3463)
                     /locus_tag="VF958_01570"
     CDS             complement(1808..3463)
                     /locus_tag="VF958_01570"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HEX9147826.1"
                     /translation="MSRRGGLAAILLALLLLWLVGWPLLVTLGEALEVPRWTLSHFAE
                     FAKRPEEWRALWASLWISLATVGLAAAIGIPLAFLFERMEFPGRRVLGALVALPVVLP
                     PLVGVIAFLFLYGESGFVARALQALLSLEESPWRLSGPAAVLLVHAYSMYVYFYLFTR
                     AGLARLDASVLEAAASLGASRGRILRRVTLPLLRPALTGAALLTFMTALASFSAPYVF
                     GGGFRVMTTQIVASKLNGDIAMAMVETVTLAAVALVGLFLLQWSERGRGVTGGGRGIP
                     PARRRLERSRWVAAALGWLLALVLLLPHATLVLVSFVPRGTWTVEILPPVLDLSNYAS
                     LLSEPERLRPLVNSLWMAVAATAAALALGFAAARLAVRAKSRFRGMLETLLTIPWALP
                     GTVFAVALSAAFSVHAPWIGRFVLVGTAAILPLAYLVRSLPLTGRAAFAGFRQLDPAL
                     DEAAASLGASRWRRLVRVDLPLLAPALTAGASLAFITALGDFVVSIVLYTFETRPISI
                     EILSSLRLNETGVAAAYGVVLATLSAVVFFVWGRRGDAVRPPG"
     gene            complement(3460..4554)
                     /locus_tag="VF958_01575"
     CDS             complement(3460..4554)
                     /locus_tag="VF958_01575"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX9147827.1"
                     /translation="MGPGGAGEGEVTAFLVLDRVDKHFGGLHVTRDVSLSVAKNEILV
                     LLGPSGSGKTTVLRIVAGLEAPDSGRVLVEGEDVTPLPPERRRFGVVFQHYALFPHMT
                     VAGNVAFGLESSGVRGGELRARVAEALAAVDLSGFEGRKVTEISGGQQQRVALARALA
                     PRPRVLLLDEPLSNLDPTLRGRTRRELKRIIRDVGITTVFVTHEQEEAFELGDRVALM
                     NAGRLEQVGAPRELYDSPATRFAAGFIGRSSVVAARWEKASGRVRLPGGAGWAAAADG
                     DLPDGAPVDVVVRPEALAFSTARDGTGVEGRVVETRFAGRETYWAVALEGGTEVEVLA
                     APDATRAGWKVFVAPAPGGPVPRAYLRSSE"
     gene            complement(4521..5549)
                     /locus_tag="VF958_01580"
     CDS             complement(4521..5549)
                     /locus_tag="VF958_01580"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEX9147828.1"
                     /translation="MTIQHVRRLLILSTLALSACRGDARTPIVLYSPHGRDLLTLVEK
                     TYESKHPNVDVRWLDMGSQDVYDRVRAEKANPRGDVWFGGPNLVFDNGARDGLLEPFE
                     PTWAASIPSESRHPQKLYFGLYRTPVVIVWNSAAVSAADAPKDWDDLLDPRWKKKILI
                     RDPLASGTMRAVWGAILERSIERTGSVESGLDWLRRLDAQTKEYVFNPSILLEKLVRR
                     EGLITIWDLPDTLFEMRHGPLAYGFPTSGTPVIDDAVGLIHGAPHAVAARELIEWLGT
                     PEAQKLAAEKAFRLPARTDLPAGELPEWARGVEKALVPAKLDWDRVGREGPAWMSRWD
                     REVRGKGK"
BASE COUNT          953 a         1979 c         2018 g          769 t
ORIGIN      
        1 catccggatc ggaggggcag ctgcggaagg acgacccgtg gggtgcggcg tgcatcgggc
       61 atgatgcgcc ctctccggcg gcggcgcagc agctcatcgc acagcgttcg cccgccgcgg
      121 ccgggacgag cggcgcgagc atcgcgagct gtgtcgcgag ggcaacgagg atcgcggcca
      181 gcgatgtcgg gcggagtcgc acgattcggg ggattgtatg tcccgggacc cgcgttgcga
      241 cttcgggagg caaccctgcg gaagctcgcg gcaagcctgg cgctcgtcgg tctcggcgta
      301 gcgtgtcgtg cgccggcgca atccgcgccg gagatcgcgc tctcgacggt ggtcacgggc
      361 ctggatcggc cgacggcgat cgcgcacgag ggagattcgc gcctcttcct gacgctccaa
      421 cgcggccgga ccgtgatcgt cgaggagggt cgcatcctcc cggcggcgtt cctcgacatc
      481 cagtcgctcg tctcgtgctg tggcgagcag ggtcttctcg ggctggcatt tcatccgcgc
      541 tacgccgaga acggcttctt cttcgtcgac tacacgaaca cctcggggaa caccgtgatc
      601 gcgcgatacc gcgtttcacc gtcgaacccg aacgccgggg acgcggcgag cggcgtcgtc
      661 ctcctgacga tcccgcagcc gttctccaac cacaacggcg gacagctcgc gttcgggccg
      721 gacgggtacc tctacatcgg catgggcgac gggggttcgg cgaacgatcc gatgtgcaac
      781 gggcagcgcg atgactcgct gctcggaaag atcctgcgga tcgacgtgga cgcgaacgtc
      841 gataccccgc cgtactacgg aattccgccg gacaacccgt tctccgcgcc gggcggcgcg
      901 cgggacgaga tctgggcgaa aggcctgcgc aacccgtggc ggttctcgtt cgaccggcag
      961 acgggagacc tgtacatcgg cgacgtcggg cagggcgctc gagaggaagt ggacttccag
     1021 gccgcgggaa gcgcgggcgg gcagaactac ggctggaagg tgatggaagg gacggcgtgc
     1081 ggcgggggag ggacgtcggg atgtccctcc agcacggccg cctgcaactc accgggtctc
     1141 acgccgccga tcctcgagta tccgcactcc ggcggcgact gttcgatcac cgggggtcac
     1201 gtctaccgcg gaccgttctt ccccgagctc gccgggacgt acctgtacgg cgactactgc
     1261 acgggaaaaa tctggggggc gacccgcggt ccgaccggaa cgtggtcggc gcggctcttc
     1321 tctgcgcacg cggccggcct gacgacgttc ggcgagggcg cggccggcga gatctacctg
     1381 gccacggaga gcgggctgct cgcgcgaatc gtgaacgcga acccggtcgc gccgacgatc
     1441 gactccgtcg agccggcttc cggttcgacg cgcggcggcg aatccgtgat tgtcacgggc
     1501 gggagcttcg tgctcggagt caccgtctcg ttcgaaggag tgacggcgcc gtccaccgtg
     1561 attctcgatc cgaacgggac gcgcctcctg gtcgtgacgc cgccgcacgc cgccgggccg
     1621 gtcgacgtcg tcgtcaggaa ccccgacggc aagagcgcgg cgctcacgtc gggatacggg
     1681 tacgccgctc cgtcgcgcgt cgcgccgccg ccccggattc cgcgcaccgt gactcgaccc
     1741 tgagaacttc ttcagccgaa aggcgactgt gatcgtcatg tggcaagtgg agacctgacc
     1801 ccatctctca gccgggcggg cggacggcgt cgccgcggcg cccccacacg aaaaagacca
     1861 ctgccgaaag cgtcgccagc acgacgccgt acgcggcggc gacgccggtc tcgttcaagc
     1921 gcagcgagga gaggatctcg atcgagatgg gccgcgtctc gaaggtgtag agcacgatcg
     1981 acacgacgaa gtctcccagg gcggtgatga aggcgaggct cgcgcccgcc gtgagcgccg
     2041 gagcgagcag cggcaggtcg acgcgcacga gcctcctcca gcgcgaggcg ccgagtgacg
     2101 cggccgcctc gtcgagcgcg ggatcgagct gtcgaaaccc ggcgaaggcc gcgcgtccgg
     2161 tcagcgggag gctccgaacg aggtacgcga gcgggaggat cgctgcagta ccgacgagca
     2221 cgaaccggcc gatccatggc gcatgaacgg aaaacgccgc ggacagcgcc acagcgaaga
     2281 ccgtgccggg cagcgcccac gggatcgtca ggagagtctc gagcattccc cggaaacgcg
     2341 acttcgctct caccgccagt cgcgccgccg cgaacccgag agcgagcgct gcggccgtcg
     2401 ccgccaccgc catccagagc gagttcacga gcgggcgaag cctttcgggc tcggagagga
     2461 gcgatgcgta gttcgagagg tcgagaaccg gaggcaggat ctcgaccgtc caggtccccc
     2521 gcggcacgaa ggagacgaga acgagcgtcg catggggaag gaggagcacc agagcaagca
     2581 gccatccgag cgcggccgcg acccaccgcg accgctcgag acggcgccga gcgggcggaa
     2641 tcccgcgtcc accacccgtg accccgcgac ctcgttcgct ccactgcagt aggaagagac
     2701 cgacgagcgc gaccgccgcg agcgtcaccg tctcgaccat cgccatcgcg atgtcgccgt
     2761 tcagcttcga cgcgacgatc tgcgtggtca tcacccgaaa gccaccgccg aacacgtacg
     2821 gcgccgaaaa ggaagcgagc gccgtcatga acgtcagaag cgccgcgccg gtgagcgccg
     2881 gccggaggag cggcagcgtg acccgccgga ggatcctgcc gcgtgacgct ccgaggctcg
     2941 cggccgcttc caggaccgaa gcgtcgagcc gcgcgaggcc cgctcgcgtg aaaaggtaga
     3001 agtagacgta catcgagtaa gcgtgcacga ggagcacggc tgcgggcccg gagaggcgcc
     3061 acggcgactc ctccagggag agcagcgcct ggagggcgcg tgcgacgaag ccgctctcgc
     3121 cgtacaggaa gagaaacgcg atcacgccga cgagcggggg cagcacgacc ggaagggcca
     3181 cgagcgctcc cagaacacgc cgtccgggaa attccatccg ttcgaagagg aacgcgagcg
     3241 ggatcccgat cgccgcggcg agcccgaccg tcgcgagcga gatccagagg ctcgcccaga
     3301 gggcgcgcca ctcttcggga cgcttcgcga actccgcgaa gtgcgagagc gtccagcgcg
     3361 ggacctcgag cgcctcgccc agggtcacga ggagcggcca cccgacgagc cacaggagga
     3421 ggagcgcgag caggatcgcg gcgaggccgc cccggcgact cattcggagg agcgaaggta
     3481 ggcgcgcggc accggacctc ccggcgcggg cgcgacgaag accttccatc cggcgcgcgt
     3541 cgcatcgggc gcagcgagca cttcgacctc ggttccgcct tcgagcgcga cggcccagta
     3601 cgtctcgcgc cccgcgaacc tcgtctcgac gacgcgcccc tccacccccg tcccgtcgcg
     3661 cgcggtcgag aaggcgagcg cctcgggacg cacgacgacg tccaccggcg ccccgtcggg
     3721 gaggtcgccg tccgcggcgg cggcccagcc ggcgcctccc ggcagccgca cgcggcccga
     3781 ggccttctcc caacgggcgg cgacgacgct cgagcggccg atgaagcccg cggcgaatcg
     3841 ggtcgcgggg gagtcataga gctcgcgcgg ggctccgacc tgctcgaggc ggccggcgtt
     3901 catgagagca acgcggtcgc ccagctcgaa cgcctcctcc tgttcgtgcg tcacgaagac
     3961 cgtcgtgatc ccgacgtcgc ggatgatccg cttcaactct cttcgcgtgc gcccgcggag
     4021 ggtcggatcg aggttcgaga gcggttcgtc gaggaggagg acgcgggggc gcggcgcaag
     4081 ggcgcgcgcg agagccacgc gctgctgctg tccgcccgag atttcggtca cctttcgccc
     4141 ttcgaatccg gacaggtcga cggccgcgag ggcctcggcg acccgcgcgc gcagctcccc
     4201 cccgcggact cccgacgact cgagaccaaa ggccacgttg ccggccaccg tcatgtgcgg
     4261 gaagagcgcg tagtgctgga agacgacgcc gaatcggcgg cgctccggcg ggagcggcgt
     4321 cacgtcctcg ccctcgacga gcacgcgtcc ggagtcgggc gcctcgagtc cggcgacgat
     4381 gcgcaggact gtcgtcttcc cgctgccgga ggggccgagg aggacgagaa tctcgttctt
     4441 cgcgaccgag agcgagacgt cacgcgtcac gtggaggccg ccgaagtgct tgtcgacccg
     4501 atcgaggacg agaaacgccg tcacttcccc ttcccccgca cctcccggtc ccaccgcgac
     4561 atccacgcgg gtccttcgcg cccgacccga tcccagtcga gtttcgccgg cacgagcgcc
     4621 ttctcgacgc cgcgcgccca ctccggaagc tctcctgcgg gcaggtccgt tcgcgccggc
     4681 agccggaacg ccttctcggc ggcgagcttc tgcgcctccg gggtcccgag ccactcgatc
     4741 aactctctcg ccgcgacggc gtgcggcgca ccgtggatca ggccgacggc gtcgtcgatc
     4801 actggcgtgc cgctcgtcgg aaacccgtac gccagcggtc cgtggcgcat ctcgaagaga
     4861 gtgtcaggga ggtcccagat ggtgatcaac ccttcgcgcc ggacgagctt ctcgaggagg
     4921 atcgacggat tgaagacgta ctccttcgtc tgcgcgtcga gtcggcgcag ccagtcgagg
     4981 ccgctctcga cgcttccggt ccgctcgatc gaccgctcca gaatcgctcc ccagaccgcg
     5041 cgcatcgtgc cggaagcgag cggatcgcgg atcaggatct tcttcttcca gcggggatcg
     5101 aggaggtcat cccagtcctt cggcgcgtcc gcagcggaca ccgcggcgct gttccagacg
     5161 atgacgaccg gcgttcggta gagcccgaag tagagcttct gcgggtggcg gctctcggaa
     5221 gggatggacg ccgcccacgt cggctcgaac ggctcgagca gaccgtcgcg cgcgccgttg
     5281 tcgaagacga ggttcggccc gccgaaccag acgtcccccc gcgggttggc tttctccgcg
     5341 cggacgcggt cgtagacgtc ctgcgagccc atgtcgagcc agcggacgtc cacgttcgga
     5401 tgcttcgact cgtacgtctt ctccacgagc gtcagcaggt cgcgcccgtg cggagagtag
     5461 aggacgatgg gagtccgcgc gtcgccgcgg cacgcggaga gcgccaatgt ggaaaggatc
     5521 aggaggcgtc ggacgtgttg gatcgtcatt ccggtttcgg cgcggaatcg taacccggcc
     5581 cgcagtgccg cagggctcgg cgttcctctg gcggtggtcc tccgacggcg gcaagacgtg
     5641 gcgctcgctg acctcgaagc tcccggtcaa ctgccagatc gagcagatcg acttccgcgc
     5701 caacaagtac gcgggcatc
//
LOCUS       DASWAV010000070         4769 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U2665
            k141_523688, whole genome shotgun sequence.
ACCESSION   DASWAV010000070 DASWAV010000000
VERSION     DASWAV010000070.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960316
            Sequence Read Archive: SRR10260007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 4769)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4769)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:47:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,929
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4769
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_523688"
                     /isolate="SMAG_U2665"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.36444 N 132.31889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..208)
                     /locus_tag="VF958_01585"
     CDS             complement(<1..208)
                     /locus_tag="VF958_01585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9147829.1"
                     /translation="MRGRALLLVAFASVAWGCAGAPVSAPPAPPKSRAPYAAPPAARH
                     QLEITIDARAARDLLAFLSRPDFQP"
     gene            complement(205..909)
                     /locus_tag="VF958_01590"
     CDS             complement(205..909)
                     /locus_tag="VF958_01590"
                     /inference="COORDINATES: protein motif:HMM:NF024811.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase-like protein"
                     /protein_id="HEX9147830.1"
                     /translation="MSGSSSNGARLVLFDVDGTLLSAGRAARESILAALSAVLGWEGS
                     ADGHDFSGKTDPQILRELVLDAVETTRFESTLDAVLDRYLRELALRLTPRAVVPKPGV
                     PALLERLAREPRVMLALLTGNIERGARMKLEPPGFNRFFPFGAFGNDSADRYSLPAIA
                     VARARERTGRDFSGESVVIIGDSVHDVACGRSLGARAIAVATGPTPAEKLAAERPDAL
                     FADFSDVEAAASAILR"
     gene            complement(906..1796)
                     /locus_tag="VF958_01595"
     CDS             complement(906..1796)
                     /locus_tag="VF958_01595"
                     /inference="COORDINATES: protein motif:HMM:NF012958.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase-fold protein"
                     /protein_id="HEX9147831.1"
                     /translation="MTGRRLLGAMGAVATLAIACATTRGVPGLSYDSFPTASLPSPAR
                     CLVLEPPSYRSVPTRSYPLLVFLHDGYGDVRTLERRGVAADLAARMADGRLPEFLVVA
                     PGAPGSWFSDSADGKRRWEEFLSGDFLRQIESRYRVLPGAASRGITGISMGGFGAVKT
                     ALKQPALFGTVSSLSGALIPIGIEDIRRYGWITGFTLKRVFGNPPDAKTLAANDVWEL
                     LHAKTFEHPPFVAHLRAGTEDFYGLDRVATQFGTYMNEHGIPTEIVLEPGGHDWNYWR
                     RALVAIAEWHAKRFSYDRGR"
     gene            complement(1822..2610)
                     /locus_tag="VF958_01600"
     CDS             complement(1822..2610)
                     /locus_tag="VF958_01600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9147832.1"
                     /translation="MNIRSSLSLAIASLAVSSLLSTARPAFADEKADQVARGLVTALG
                     GQSAWEKARQLRFDFIVEREGTRAAEFHHVWDRYTGDYRLLGTDKSGAPFAVYFNVNT
                     REGVAFMNGKPVEGDEKKKMLEMAYGRFINDSYWLLAPWKVFDPGVHRDYDGEKTGPD
                     GALCDVLRLSFDGVGLTPKDIYWLWITRDGRHMVQWQYVLGGAQEEPTTALWKDWRTF
                     GGIAVSLEKEIVGRPAKIRFDNVAVSPSRDDKDFAPPAAAPSPR"
     gene            2609..3118
                     /locus_tag="VF958_01605"
     CDS             2609..3118
                     /locus_tag="VF958_01605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9147833.1"
                     /translation="MRGSYGRKLPFVELQRLPREIELALSGAGFRARRRLAMPFLTVD
                     PGGFPRAALLTLGEVRARSRVLLAVAVRAGSRTAANLIRRRTATLFYLARNRAVWVQA
                     RAGRARVSDADPDRQIFPLTIVNVKVDRPAPEEGAVALAAGPTFMLAGSSRLFSEELY
                     AELGAGPGR"
     gene            3115..3849
                     /locus_tag="VF958_01610"
     CDS             3115..3849
                     /locus_tag="VF958_01610"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX9147834.1"
                     /translation="MKFRDRTVLVTGASRGIGRSIALRFAEEGARVALVSRTVAELLQ
                     TASLVDKAGAKTIAIPTDIRDREGVAASVAKAESELGPIDVLVNNAGVFLWRPFLKLS
                     AEEWDLVIATNLTGAANFCRAVLPGMMSRRRGRIVNVSSIHGMRGEANLAAHSAAKFG
                     LIGMTQSLAREFREYNIAVNAVCPGTVENKLDETGAADRAEPLAEKLWPRDVARTVLF
                     LASDDAAAITGAALEVLGGTHLAIQP"
     gene            complement(3946..4305)
                     /locus_tag="VF958_01615"
     CDS             complement(3946..4305)
                     /locus_tag="VF958_01615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017653437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HEX9147835.1"
                     /translation="MQMLGLVAVGVVAGVAAGMFGIGGGLIIVPALVLLYGMTQHSAV
                     GTSLGAILLPVGALGAWVYWKNGNLNVRYSLLIAAGLLVGAFLGAKLVEPVSDLTLRR
                     LFGGFLLLISIKMILGK"
     gene            complement(4319..>4769)
                     /locus_tag="VF958_01620"
     CDS             complement(4319..>4769)
                     /locus_tag="VF958_01620"
                     /inference="COORDINATES: protein motif:HMM:NF015000.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HEX9147836.1"
                     /translation="GRSLAYVVEVKASPGYDRGVMGRRIAGLAAEYGVAGRVLVASFD
                     AEFLKRMREIEPRLATSYVFDHPVALPEPARLTPLFPPVDAIAPAKDLATPPLIAQAA
                     AAGLSVHPWTVNDPEEIRRFLAAGVASITTDDPETALALRNGAAAGQ"
BASE COUNT          772 a         1628 c         1671 g          698 t
ORIGIN      
        1 cgggttgaaa gtccggccgc gacaggaatg ccagaaggtc ccgggcggcg cgcgcatcga
       61 tcgtgatctc aagctggtgg cgggccgccg gcggcgccgc gtagggcgcg cggctcttcg
      121 gcggagcagg aggcgcggaa accggcgctc ccgcgcagcc ccaggccacg gaagcgaaag
      181 cgacgagcag aagcgcgcgg cccctcatcg caggatcgcg ctcgctgccg cttcgacgtc
      241 ggagaagtcg gcgaacaggg cgtccggccg ctccgccgcg agcttttccg cgggggtggg
      301 tccggtcgcc accgcgatgg cccgtgcgcc gagcgagcgc ccgcacgcga catcgtgcac
      361 cgaatccccg atgatgacga ccgactcccc ggagaagtcc cggcccgtcc gctcgcgggc
      421 gcgggcgacg gcgatcgcgg gaagggagta gcggtccgcg gaatcgtttc cgaacgcgcc
      481 gaacgggaag aagcggttga agccgggggg ctcgagcttc atccgggcac cgcgctcgat
      541 gttgccggtg agaagtgcca gcatgacccg cggttccctc gcgaggcgct cgaggagagc
      601 gggcaccccc ggcttgggga cgaccgcgcg cggcgtgaga cggagcgcga gctcgcgaag
      661 gtagcggtcg agcactgcgt ccagcgtgct ctcgaaccgt gtcgtctcga ccgcgtcgag
      721 gacgagctcc cggagaatct gaggatcggt cttgccggag aagtcgtgcc cgtcggcgga
      781 gccttcccag ccgagcactg ccgagagtgc ggcgaggatc gactcgcgcg cagctcgccc
      841 cgccgaaagc agggtgccgt cgacgtcgaa gagaacgaga cgcgctccat tcgagctact
      901 gccgctcatc ggccgcgatc atacgagaac ctcttcgcgt gccactcggc gatcgcgacg
      961 agagcgcgtc gccagtagtt ccagtcgtgt cctccgggct cgagcacgat ttccgtcgga
     1021 atgccgtgct cgttcatgta tgtgccgaac tgagtcgcga cgcgatcgag cccgtagaag
     1081 tcctcggtgc cggcgcgcag gtgtgccacg aaaggagggt gctcgaacgt cttcgcgtgg
     1141 agaagttccc agacgtcgtt cgccgcgagc gtcttcgcat cgggagggtt cccgaacacg
     1201 cgcttcaacg tgaaaccggt gatccacccg tagcggcgga tgtcctcgat accgatgggg
     1261 atgagcgcgc cggaaagcga agacaccgtg ccgaagaggg ccggttgctt gagagccgtc
     1321 ttcaccgcgc cgaagccgcc catggagatg ccggtgatcc cgcgcgacgc cgcccccgga
     1381 agcacgcgat aacgcgactc gatctgtcgg aggaagtcgc cggagaggaa ctcctcccac
     1441 cggcgcttgc cgtccgcgga gtcggaaaac caggatccgg gagcacccgg cgcgacgacg
     1501 aggaactcgg gcagtcgtcc gtccgccatg cgggcggcga ggtcggcggc gacgccgcgg
     1561 cgctcgagtg tccgcacgtc cccgtagccg tcgtgcagga agacgaggag cggatagctc
     1621 cgcgtgggaa cggatcggta cgaggggggc tccaggacca ggcagcgcgc gggcgaaggc
     1681 aggctcgccg tcgggaacga gtcgtacgaa aggccgggga cgccgcgcgt ggtcgcgcag
     1741 gcgatggcga gagtcgccac cgcccccatc gccccgagca acctccgccc cgtcatcccg
     1801 agggaagcga gggatctcgc cttaccgggg cgacggcgca gccgcgggcg gcgcgaaatc
     1861 cttgtcgtcg cgcgacggtg agaccgcgac gttgtcgaac cggatcttcg cggggcggcc
     1921 gacgatctct ttctcgagcg agaccgcgat ccccccgaac gtccgccaat ccttccagag
     1981 cgcggtcgtc ggctcttcct gcgccccgcc gaggacgtac tgccattgca ccatgtgccg
     2041 gccgtcccgg gtgatccaca accagtagat gtccttcggt gtcaggccga cgccgtcgaa
     2101 cgagagacgc agcacgtcgc agagggctcc gtcgggtccc gtcttctcgc cgtcgtagtc
     2161 gcggtggacg ccggggtcga acaccttcca cggcgcgagc agccagtacg agtcgttgat
     2221 gaagcgcccg tacgccattt cgagcatctt cttcttctcg tcgccctcga ccggttttcc
     2281 gttcatgaac gcgacgccct cgcgcgtgtt cacgttgaaa tagaccgcga acggcgcacc
     2341 cgacttgtcc gtgccgagga gccgatagtc gccggtgtag cggtcccaga cgtggtgaaa
     2401 ctccgcggcg cgcgtgccct cgcgctcgac gatgaagtcg aagcgcagct ggcgtgcttt
     2461 ctcccacgcg gattggccgc cgagcgccgt gacgagcccg cgcgcgacct ggtcggcctt
     2521 ctcgtcggca aacgccggac gcgccgtgga gaggagggaa gaaaccgcga ggctggcgat
     2581 cgccagagag aggctgctgc gaatgttcat gcggggatca tacggccgta aactcccctt
     2641 cgtggagctg cagaggctgc ctcgtgagat cgagctggcg ctctccggcg ccggtttccg
     2701 agcgcgccga aggctggcaa tgcccttcct gaccgtggac ccgggcggct tccctcgcgc
     2761 cgcgctgctc accctgggag aggtccgcgc gcgctcccga gtcttactcg ccgtcgccgt
     2821 ccgcgccgga agtcgcacgg cggccaacct catccgccgt cggaccgcga cgctctttta
     2881 tctcgcgcgc aaccgggccg tctgggttca ggccagggcg gggcgcgcac gcgtttcgga
     2941 tgccgatccc gaccggcaga tcttcccgct gacgatcgtg aacgtgaagg tggaccgacc
     3001 cgccccggaa gagggcgccg tcgctctcgc cgccgggcct actttcatgc tcgccggctc
     3061 gtcgcggctc ttttccgagg agctctacgc cgagctcggc gcggggcccg ggcgatgaag
     3121 ttccgcgacc gcaccgtgct cgtgacgggc gcctcgcgcg ggatcggccg ctcgatcgct
     3181 ctgcgcttcg ccgaggaagg ggcgcgggtc gcgctcgtct cccggaccgt cgccgaactc
     3241 cttcagaccg cgagcctcgt cgacaaggcg ggcgcgaaga cgatcgcgat tccgaccgac
     3301 atccgcgacc gcgagggtgt cgcggcgtca gtggcaaagg ccgagtcgga gctcgggccg
     3361 atcgacgtcc tcgtgaacaa cgccggggtc tttctctggc ggccgttcct gaagctgtcc
     3421 gccgaggagt gggacctcgt gatcgcgacg aacctgacgg gcgcggcgaa cttctgccgc
     3481 gcggtgctgc cggggatgat gtcgcgccgg cgcgggcgca tcgtcaacgt ttcctcgatc
     3541 cacggaatgc gcggcgaagc gaacctcgcc gcccacagcg ccgccaagtt cgggctgatc
     3601 ggcatgacgc agagcctcgc ccgggagttc cgcgagtaca acatcgcggt gaacgcggtt
     3661 tgtccgggta ccgtcgagaa caagctcgac gagaccggcg cggccgaccg tgccgagccg
     3721 ctcgcggaga agctctggcc gcgggacgtc gcgcgcaccg tcctgttcct ggcctccgac
     3781 gacgccgctg ccatcacggg cgcggcgctc gaggtgctcg gcggaaccca cctcgcgatc
     3841 cagccgtagg gtccggtctt caagggtcga gaaagagcgg aatcgacctg agtggtcttg
     3901 ccatctttgc gaagcgaaat ggcaagtgga gacctgaccc cgtccctact tccccaggat
     3961 catcttgatc gagatgagaa gcaggaaccc tccgaagagc cgccgaagcg tcaggtccga
     4021 gaccggttcg accagcttgg cgccgaggaa ggcgccgacc agaaggcccg cggcgatcag
     4081 gagcgagtag cgcacgttca ggttgccgtt cttccagtag acccacgcgc cgagcgcgcc
     4141 gaccggcagc aggatcgcgc cgagcgacgt cccgaccgcc gagtgctgcg tcatgccata
     4201 gagcagcacg agcgcgggga cgatgatcag gccgccgccg atcccgaaca ttcccgccgc
     4261 gacgcccgcg acgactccga ccgccaccaa cccgagcatc tgcatcggcg tgtcctttct
     4321 attggccggc cgccgctccg ttgcgcagcg cgagcgctgt ctccggatcg tcggtcgtga
     4381 tcgacgcgac cccggcggcg aggaacctcc ggatctcctc gggatcgttc accgtccagg
     4441 ggtgcacgga gagtcccgcc gcggcggcct gcgcgatcaa agggggcgtc gcgaggtcct
     4501 tcgccggcgc gatcgcgtcg accggcggga agagcggagt gagacgcgcc ggctcgggca
     4561 gcgccacggg gtgatcgaag acgtagctgg tcgcgagcct gggctcgatc tcgcgcatgc
     4621 gtttcaggaa ctctgcgtcg aaggaggcca cgaggacgcg cccggcgaca ccgtactccg
     4681 cggccaggcc ggcgattcgg cggcccatca ccccgcggtc gtaaccgggt gaggccttca
     4741 cctcgacgac gtaggcgagc gagcgccca
//
LOCUS       DASWAV010000071         1641 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U2665
            k141_484357, whole genome shotgun sequence.
ACCESSION   DASWAV010000071 DASWAV010000000
VERSION     DASWAV010000071.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960316
            Sequence Read Archive: SRR10260007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 1641)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1641)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:47:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,929
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1641
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_484357"
                     /isolate="SMAG_U2665"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.36444 N 132.31889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            42..>1641
                     /gene="ctaD"
                     /locus_tag="VF958_01625"
     CDS             42..>1641
                     /gene="ctaD"
                     /locus_tag="VF958_01625"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014784210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit I"
                     /protein_id="HEX9147837.1"
                     /translation="MSAVVQEPEHPEPRVNYLNATYGIRSWLLTRDHKRIALLYLGSI
                     TFFFFLGGLFALLIRLELLTPAGDLVQAETYNKLFTMHGVTMIFFFLIPSIPAVLGNF
                     LIPLMIGAKDLAFPRLNLLSWYVYMVGAFFTLWAVVHGGVDTGWTFYTPYSTASSNSN
                     VIAAAVGIFITGFSSILTGLNFIVTIHTMRAPGMSWFRLPLFVWGHYATSLIFVLGTP
                     VIAVTILMVFVERVFHLGLFDPALGGDPILFQHLFWFYSHPAVYIMILPAMGVVSELV
                     TTFSRKNIFGYKFVAFASIAIAVLGFLVWGHHMFVSGQSVYAGLIFSLLSVLVAIPSA
                     VKVFNWTATMYRGSISYRTPMLYAFGFIGLFTIGGLTGLFLATLGVNVHVHDTYFIIA
                     HFHYIMVGGAIMGYLGGMHYWWPKISGKLYPEGWARFSALVVFIGFNLTFFPQFILGY
                     LGMPRRYHAYPPEFQVLNVMSTAGASILGVGYVIPMIYLVWSMRYGKNAPANPWGSTG
                     LEWKTTSPPPTDNFEVPPVVTEDPY"
BASE COUNT          248 a          561 c          476 g          356 t
ORIGIN      
        1 ccgccgacgg ccgcgcccct ctccaaggag aacgtgaact gatgtccgcc gtcgtgcaag
       61 agcccgagca tccggagccg cgcgtgaact acctgaacgc cacgtacgga atccgctcgt
      121 ggctgctgac gagggaccac aagaggatcg cgctcctcta tctcggctcg atcacgttct
      181 tcttcttcct cggcggtctc ttcgcgctgc tcatccgcct ggagctcctg acgcccgccg
      241 gcgacctcgt gcaggcggag acctacaaca agctcttcac gatgcacggc gtgacgatga
      301 tcttcttctt cctgatcccg tcgatcccgg cggttctcgg caatttcctg atcccgctga
      361 tgatcggagc gaaggacctc gcgttcccga ggttaaacct gctctcctgg tacgtctaca
      421 tggtcggggc gttcttcacg ctctgggccg tcgtgcacgg cggggtggac accggctgga
      481 ccttctacac cccctacagc acggcgtcgt ccaactcgaa cgtcatcgcg gcggccgtcg
      541 ggatattcat caccgggttc tcctcgatcc tgacgggcct gaacttcatc gtgacgatcc
      601 acacgatgcg cgcgccggga atgagctggt tccggctgcc gctcttcgtg tgggggcact
      661 acgcgacgag cctcatcttc gttctcggca cgccggtcat cgccgtgacg atcctgatgg
      721 tcttcgtcga gcgcgttttc cacctgggcc tcttcgaccc ggcgctcgga ggcgacccga
      781 tcctcttcca gcacctgttc tggttctact cgcacccggc cgtctacatc atgatcctgc
      841 cggcgatggg cgtggtctcg gagctcgtca cgacgttctc gcgcaagaac atcttcggct
      901 acaagttcgt cgcgttcgcg tcgatcgcga tcgccgtgct cgggttcctc gtctggggac
      961 accacatgtt cgtgtcgggc cagtcggtct acgcgggcct gatcttctct ctcctctcgg
     1021 tcctcgtcgc gattccctcg gccgtcaagg tcttcaactg gacggccacg atgtaccggg
     1081 gatcgatctc gtaccggacg ccgatgctct acgcgttcgg gttcatcggt ctcttcacga
     1141 tcggcgggct gacggggctc ttcctggcga cgctcggagt gaacgtgcac gtccacgaca
     1201 cgtacttcat catcgcgcac ttccactaca tcatggtggg cggcgcgatc atggggtatc
     1261 tcggcggcat gcactactgg tggccgaaga tctccggcaa gctctacccc gagggctggg
     1321 cgcgtttttc ggcgctcgtc gtcttcatcg gcttcaacct gacgttcttc ccgcagttca
     1381 tcctcgggta cctcgggatg ccgcggcgat accacgccta tcccccggag ttccaggtct
     1441 tgaacgtgat gtccaccgcg ggggcctcca tcctgggcgt gggttacgtc attccgatga
     1501 tctacctcgt ctggtccatg cggtacggaa agaacgcccc ggcgaacccc tggggatcga
     1561 cggggctcga gtggaaaacc acgtccccgc cgccgacgga caacttcgag gtgccgcccg
     1621 tcgtgacgga agacccctac a
//
LOCUS       DASWAV010000072         3722 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U2665
            k141_484374, whole genome shotgun sequence.
ACCESSION   DASWAV010000072 DASWAV010000000
VERSION     DASWAV010000072.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960316
            Sequence Read Archive: SRR10260007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 3722)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3722)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:47:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,929
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3722
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_484374"
                     /isolate="SMAG_U2665"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.36444 N 132.31889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1746
                     /locus_tag="VF958_01630"
     CDS             <1..1746
                     /locus_tag="VF958_01630"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX9147838.1"
                     /translation="MRFAFRLNLILVLLAAGLATLLSVVAVRLTDRAVEDRARDRLRR
                     EIDLLADEVEARLPYPDRLDEFVRRAAERLGVRVTVIAPDGRVLDESGVSPNDVAAME
                     NHAARPEVVQARAEGYGTSVRRSATLESDLLYLARPIGGDRTGGPILRLAIPLADLRR
                     IEAKYEWILGGAVLVACALLVVAGSVAVGRLARPIRHVTEAALAVARGDLGREGPEAG
                     PQEILELSSALKRMKGSLLQSVERAEGERRLAAAVFETLPDGVVVVDDKLRVLDANGG
                     FRAMMSAPDPVGRPLVDLLRDRALFAPFDDALARREPVDATVRRPRDATWQVAVRPLP
                     RGSRGAAVGILRDVTALERNEAMRRRFVADVSHELRTPVASIAAAAETLAESGPDDPE
                     TPRLILLVRRQAERMRELIDDLTDLSLIESGAVGLQRENVDLRALACETAEEFRQSAG
                     TRDVSIRVEGLDRLSVQGDRGRMSQILRNLTDNAVKFSPAGGTVTLRVERADEKPVLI
                     VEDQGPGIPLQEQDRIFQRFYQVDRSRSKARPGTGLGLAIVKHLAHLHGAEVTVRSQP
                     GRGSAFRVTFREEKT"
     gene            1743..2411
                     /gene="phoU"
                     /locus_tag="VF958_01635"
     CDS             1743..2411
                     /gene="phoU"
                     /locus_tag="VF958_01635"
                     /inference="COORDINATES: protein motif:HMM:TIGR02135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0051049 - regulation of transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HEX9147839.1"
                     /translation="MKEHFAVRLEALRRRLILMSGEVEKQIRSAIEALTEGSAEKARA
                     VIANDEEIDRLENQIEEEAIQLLALEQPVAIDLRFLVGVLKINNDLERIGDHAVNIAE
                     GAERLAGRPPFAPAIDIPAMSEIVQRMLREALDAFIEKNAALAKKVIREDDIVDAKNE
                     GVIRQLLTYMAERPVLISHCLELISVSKNLERVGDLATNIAEDTIFIAEAKVVKHHGV
                     DLAG"
     gene            complement(2541..2894)
                     /locus_tag="VF958_01640"
     CDS             complement(2541..2894)
                     /locus_tag="VF958_01640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9147840.1"
                     /translation="MKRSLFALIAAGGLIAAANCATQPETKIKQEGSATVMKQTGPGE
                     NVKTTVVTGVVTKYEPGNELEIRPADGNMHDFDLEESVQIQGTIVVGQPATVTYVESG
                     GKKLVKIIAAGATTP"
     gene            complement(2964..>3722)
                     /locus_tag="VF958_01645"
     CDS             complement(2964..>3722)
                     /locus_tag="VF958_01645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9147841.1"
                     /translation="SVRPTTVNLAYPFVAPLSLRWIPITALDGLQGKPRVFVHVLDSG
                     RRLLRTFDHPLREAWTPGREQSYEIELYQSAIADRLPAGAHEVTFGLYDDAGKTRWPL
                     AIQGEEVGRCEYRLATLVVPSSAAPSPTFQFSSAWLPIEPGPSKQVVARRCLRTEGSI
                     SVLSPPSPGRVRLAASLTAGGSASPSVGWKVSASCTSESVEVTGTDLQWIGFPMVPAA
                     TEPPCEIHFTPPAPAPADARPLCLEVLAWRPATP"
BASE COUNT          621 a         1229 c         1277 g          595 t
ORIGIN      
        1 atgcgcttcg ctttccggtt gaacctgatt ctcgtcctcc tggcggccgg cctcgccacg
       61 ctgctgtcgg tggtcgccgt gcgtctgacg gaccgcgcgg tcgaagatcg cgcccgcgac
      121 cggctccgac gcgaaatcga cctcctggcc gacgaagtgg aggcccgtct cccgtacccg
      181 gaccgcctcg acgagttcgt ccgccgggcg gccgagaggc tcggcgtccg agtcactgtg
      241 atcgcgccgg acggacgcgt cctcgacgaa tccggcgtct ccccgaacga cgtggctgcg
      301 atggagaacc acgccgcgcg gcccgaggtc gtccaggcgc gggccgaagg ttacggcacg
      361 tcggtccggc ggtccgcgac cctcgagtcg gacctgctct acctcgcgcg gccgatcgga
      421 ggggatcgaa ccggcggacc gattctccgc ctcgcgatcc cgctcgccga cctgcggcgc
      481 atcgaggcga aatacgagtg gattctcggg ggcgcggtgc tcgttgcgtg cgccctgctc
      541 gtcgtcgccg gtagcgtcgc cgtcgggcgt ctcgcgcgac ccatccggca cgttacggag
      601 gccgcgctcg ccgtcgcccg tggagatctc ggccgtgagg ggcccgaggc aggtccgcag
      661 gagatcctcg agctctcgtc cgcgctcaag cggatgaagg gatcgcttct gcagtccgtc
      721 gagcgggcgg agggcgaacg ccgtctcgcg gcggcggtgt tcgaaacact acccgacggc
      781 gtcgtcgtcg tggacgacaa gctgcgggtc ctggatgcca acggcggctt ccgagcgatg
      841 atgtcggcgc ccgatccggt cggacgcccg ctcgtggatc tcctgcgcga tcgcgcgctc
      901 ttcgccccct tcgatgacgc acttgcgcgc cgcgaaccgg tggacgcgac ggttcgccgg
      961 ccgcgcgacg cgacgtggca ggtcgctgtg aggccgcttc cacggggctc acggggcgcc
     1021 gctgtgggga tcctccggga cgtgaccgcg ctcgagcgca acgaagccat gcggcggcga
     1081 ttcgtggcgg acgtctccca cgagctgcgg acgccggtcg cgtcgatcgc ggccgcggcg
     1141 gagacgctcg cggagagcgg gccggacgat cccgagacgc cgcggctcat ccttctcgtg
     1201 cggcgccagg ccgaacggat gcgcgagctg atcgacgacc tgaccgacct ctccctgatc
     1261 gagagcggcg cggtgggcct tcagcgggag aacgtggacc tgcgcgctct cgcttgcgag
     1321 acggcggagg agttccggca gtccgcgggg actcgggacg tctcgattcg cgtcgagggc
     1381 ctggatcgcc tctccgtcca gggcgaccgg gggcggatga gccagatcct gcggaacctc
     1441 acggacaacg cggtcaagtt ctcccccgcc ggcggtaccg tcacgctccg agtcgaacgt
     1501 gccgacgaga aaccggtgct catcgtcgaa gaccagggcc cgggaatccc gctgcaggag
     1561 caggatcgga tctttcagcg tttctaccag gtcgatcgct cgcgctcgaa ggcgcgcccg
     1621 ggaacgggac tggggctggc gatcgtgaag cacctcgcac acctccatgg tgccgaggtg
     1681 acggtccgga gtcagccggg ccgcgggagc gcgtttcggg tgacgttccg ggaggagaaa
     1741 acatgaaaga gcatttcgca gtccggctcg aggctttgag gcggcgtctc atcctcatga
     1801 gcggagaggt cgagaaacag atccggtccg caatcgaggc tctcaccgaa gggtccgccg
     1861 agaaggcgcg cgccgtgatc gcaaacgacg aagagatcga ccggctcgag aaccagatcg
     1921 aggaagaggc gattcagctg ctcgcgctcg agcaaccggt cgcgatcgac ctgcgattcc
     1981 tcgtgggcgt gctcaagatc aacaacgatc tcgagcgaat cggcgatcat gcggtcaaca
     2041 tcgccgaggg ggccgagcga cttgccgggc gcccgccgtt cgctcccgcg atcgacatcc
     2101 ccgcgatgtc cgaaatcgtc cagcggatgc tgcgcgaggc gctcgacgcc ttcatcgaga
     2161 agaacgcggc gctcgcgaag aaagtgatcc gcgaggacga catcgtcgat gcgaagaacg
     2221 agggggtgat ccggcagctc ctgacctaca tggccgaaag gccggtactc atctcacact
     2281 gcctcgagct catctcggta tcgaagaacc tggagcgcgt cggcgacctc gcgaccaata
     2341 tcgccgagga cacgatcttc atcgccgagg cgaaggtcgt aaaacatcac ggcgtcgacc
     2401 tggccggata gagaagaagc ggggtcaggt cttcatgaag acaacgcgcg ggcggctgag
     2461 ccgaggcctc cttgccacat cgccaatgac gtggcaagtg gagacctcaa cccaaggtgg
     2521 caagtggaga cctgaccccg ttatggagtc gtcgcgccgg cggcgatgat cttcaccagt
     2581 ttctttccgc ccgactcgac gtaggtgacc gtcgcgggct ggccgaccac gatcgttccc
     2641 tggatctgaa cgctttcttc caggtcgaag tcgtgcatgt ttccgtcggc gggacggatc
     2701 tcgagctcgt tgcccggctc gtacttcgtc accacgccgg tcacgaccgt ggttttcacg
     2761 ttctcccccg gccccgtctg tttcatgacg gtcgccgagc cttcctgctt gatcttggtt
     2821 tccggttgcg ttgcgcagtt cgccgccgca atcagacccc ccgctgcgat gagcgcgaaa
     2881 aggctgcgtt tcattttcga cacctcctcg agaaaaactc tccgcgcccg gaatgatcaa
     2941 gatccgtgcc ggagaaggcg gagtcagggg gtggcgggcc tccaggcgag cacttccagg
     3001 cagagcggac gggcatcggc gggagccggc gcgggcggcg taaagtgaat ttcgcacgga
     3061 ggctcggtcg ccgcggggac catgggaaaa ccgatccact gcaggtccgt tcccgtcact
     3121 tcgacggatt ccgatgtgca gctcgccgag actttccagc cgactgaagg cgaggcagat
     3181 ccgcctgccg tcaggctcgc cgcgaggcgc accctcccgg gcgatggcgg tgacaggacc
     3241 gagatggacc cttcggtccg gagacatcgc cgggcaacga cctgcttcga cgggcccggc
     3301 tcgatcggca gccacgcgct cgaaaattgg aacgtcggcg aaggcgcggc ggacgaagga
     3361 acgacgagcg tcgcgagccg gtattcgcac cgtccgactt cctcgccttg gatggcgagc
     3421 ggccatcggg tcttccccgc gtcatcgtag aggccgaagg tcacctcgtg cgcacccgcc
     3481 ggcaggcgat cggcgatcgc ggactggtag agctcgatct cataactctg ttcacgcccc
     3541 ggggtccagg cctcccgcag cggatggtcg aacgtccgga ggagcctccg cccgctgtcg
     3601 agcacgtgca cgaacacgcg cggctttccc tgcagtccgt cgagggcggt aatcgggatc
     3661 cagcggagcg acagcggcgc gacgaaggga taggcgagat tgaccgtcgt cggccggacc
     3721 ga
//
LOCUS       DASWAV010000073         4055 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U2665
            k141_563589, whole genome shotgun sequence.
ACCESSION   DASWAV010000073 DASWAV010000000
VERSION     DASWAV010000073.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960316
            Sequence Read Archive: SRR10260007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 4055)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4055)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:47:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,929
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4055
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_563589"
                     /isolate="SMAG_U2665"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.36444 N 132.31889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..732
                     /locus_tag="VF958_01650"
     CDS             <1..732
                     /locus_tag="VF958_01650"
                     /inference="COORDINATES: protein motif:HMM:NF024117.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HEX9147842.1"
                     /translation="PGVWTKFSEFLQTELPELPRAVVPDAAASLRSVPKDLAEFRRIR
                     EERRARCLAPSYAALTPTAIAHAASPLPGWEATGRGMSWGRVIHQLLEGLMRNPSLDV
                     RAYATNLLAEEERPATDLDEVVTVVEGVRLSALWRRALSARRQLVEVPFALEIDSSEI
                     GRMDGPARTVLQGAIDLAFEEDSGWFIVDYKSDTISDNLEEIVAFYSRQIELYRNYWE
                     KLTGKPTRAGLYFVSTGQEVWPEIV"
     gene            complement(970..2052)
                     /locus_tag="VF958_01655"
     CDS             complement(970..2052)
                     /locus_tag="VF958_01655"
                     /inference="COORDINATES: protein motif:HMM:NF037526.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Vms1/Ankzf1 family peptidyl-tRNA hydrolase"
                     /protein_id="HEX9147843.1"
                     /translation="METTTRPPSLAQTLERLARMEPVRSPLLSLYLNAQPDSRGKDNY
                     AAFVRKELRRLVEDFPPRSSERESIERDAQAIERWLSEQVRPSANGIAIFACSATGFF
                     EALELDAPIASHRLTIGDRPHLAALARIVDRYPRHAVVLADTNHARIYVFGRGRMIER
                     EELRSEKVSRTDTGGCSQLRYQRHVEDHYLHHAKELVERLGRIVAEERAEYVLLSGDD
                     VIVPLLRSQLSKELARKVVGVLRLDIRTPENEVMKAAGDALSAHVAEEEAKRIKELHG
                     EWRGGGLAVAGTRKVLEALGNGQADEVWIATKEGEADEIVQRALATSASVRFVEDPGL
                     LAEMAGVVARLRYKAGISPMERKAEP"
     gene            2168..2920
                     /locus_tag="VF958_01660"
     CDS             2168..2920
                     /locus_tag="VF958_01660"
                     /inference="COORDINATES: protein motif:HMM:NF023197.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3313 family protein"
                     /protein_id="HEX9147844.1"
                     /translation="MNRSASRATVVLVLLIALFGAAGCIEKAKPASPAGFIPEKELEK
                     PSDLPFQKAWRKKDLDWHHYTEIYVAPWNTEYLRQMNWWEKLERGEKVKEDAKELAGY
                     ARGVFEEAFRNDRKHRFTVVEQPGPNTLIAEFALTEVIPNKIVLDALGYAAGPAVGAA
                     GTVAANVQTATTHSTVAFEARMKDGATGQVVAMFADREAEKFSLVNVKDLTWYGHAKS
                     ILREWADQFVKILNKQPGEIVKDSAPFDLRPW"
     gene            complement(3051..>4055)
                     /locus_tag="VF958_01665"
     CDS             complement(3051..>4055)
                     /locus_tag="VF958_01665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9147845.1"
                     /translation="YLERGDAGRAVELLEEAYGWDEENGLVLALLTLAYVRRADFPFA
                     RFYLDLAEQRAPRAPPQAYEILGEVYYSLNRLEDAVAAWEHFQRIGGESPTTLRQLSK
                     LRQELALASGQRHFENERFSFYWDPAISAPVVEKIADRLAAIYDEQSAFFGTDLPTAQ
                     IVILYAGRSYFSLVSVPDWVSGVFDGKIRVSLDPDGGVTPELTFVLAHELAHSFVRQA
                     SADRAPGWLHEGLAQWWEGKRIPVSEMRQEFRGRSPHSLSEMEGNLAKRADRAAARTN
                     YVQALAVVEYLFEHHGSGAVACLTRDLGEGRSFAEALRVETGLTPEELSRRWKEWAKL
                     "
BASE COUNT          677 a         1339 c         1336 g          703 t
ORIGIN      
        1 ccgggcgtct ggacgaagtt ctcggaattt ctgcaaaccg agcttccgga gttacctcgc
       61 gcagtcgtgc cggacgccgc cgcctcgctc cgctcggttc cgaaagatct cgccgagttt
      121 cggcgcattc gagaagaacg ccgcgcccgc tgccttgcgc cgagctatgc cgccctgacc
      181 cccacggcga tcgcgcacgc ggcctcgcct ctgcccggct gggaggcgac cggacgcgga
      241 atgtcctggg gtcgcgtcat tcaccagctg ctcgaagggc tcatgaggaa tccctcgctc
      301 gacgtccggg cctacgcgac gaacctcctc gccgaggagg aacgaccagc gaccgatttg
      361 gatgaagtcg tgacggtggt cgaaggagtc cggttatccg cgctctggcg gcgagcgctt
      421 tccgctcgcc ggcagctcgt cgaagttcct ttcgctctcg agatcgactc gtccgaaatc
      481 ggcaggatgg acgggccggc gcgcacggtc cttcagggcg cgatcgacct cgcgttcgag
      541 gaggactccg gctggttcat cgtcgactac aagtccgaca cgatctcgga caacctcgaa
      601 gagatcgtcg ccttctattc ccgccagatc gagctctacc gcaattactg ggagaagctc
      661 accgggaagc cgacgagggc cggactttac ttcgtttcga cgggccagga ggtctggccg
      721 gagatcgtct agcggtggtc gatgtccgtc cgggcgtcgc gttcgccttg acgctcggcg
      781 cggcggacgg cttcgaagcg gcttgcttcg agggctccga tcgcccgaag tcgggcgcct
      841 tctcccttcg atccgggcgc ttttgggaag aagccttcaa cgccgcccgg gcctgccgcg
      901 ggcccgctgc atcgtcctgc cggtcgcgac cggcggtctt gtagtggtcg ggatgaacgt
      961 tgatcttgct cacggttcgg ccttcctttc catcggcgag atccctgcct tgtagcgaag
     1021 cctcgcgacg acgccggcca tctccgcgag cagaccgggg tcctcgacga accggacgga
     1081 ggcgctcgtc gcgagcgcac gctgaacgat ctcgtccgcc tcgccttcct tcgtcgcgat
     1141 ccacacttca tccgcctggc cgttgccgag cgcctcgagg acctttcgcg tcccggcgac
     1201 cgcgagtcct cccccgcgcc actcgccgtg cagctctttg atccgcttgg cttcctcctc
     1261 ggcgacgtgc gccgagagtg cgtctccggc cgctttcatg acctcgttct ccggcgtgcg
     1321 gatgtcgagg cggagcacgc cgacgacttt gcgcgccagc tccttcgaga gctgtgaacg
     1381 cagcaggggc acgatgacgt catcgccgga cagcaggacg tactcagcgc gctcttcggc
     1441 gacgatgcgg ccaagtctct cgacgagttc cttggcatgg tgcaggtagt ggtcctcgac
     1501 gtgtcgttga taccgcagct gagagcagcc acccgtatcc gtgcgcgaga ccttctcgct
     1561 ccggagctct tcccgctcga tcatgcgacc gcgcccgaag acatagatgc gggcgtgatt
     1621 cgtgtccgcc aggacgaccg cgtggcgcgg atagcggtcg acgatccggg cgagagcggc
     1681 caggtgcggg cggtctccga tcgtcagccg gtgactcgcg atcggcgcgt cgagctccag
     1741 cgcttcgaag aagcccgtgg ccgagcacgc gaaaatggcg atcccgttcg cggaagggcg
     1801 gacctgctcg gagagccatc gctcgatcgc ctgagcgtct ctttcgatgc tctccctctc
     1861 cgatgagcgg ggcggaaaat cctcgaccag cctgcggagc tccttgcgga cgaaggcggc
     1921 gtagttgtcc tttccacggc tgtcgggctg agcgttcagg tagaggctga gaagggggga
     1981 ccgcaccggc tccattcgcg cgagccgctc gagggtctgg gcgagcgacg ggggccttgt
     2041 cgtcgtttcc atcgtggttt cccttcggat tcccggggag caagcggtgt gccggaaagg
     2101 gtcgcacgcg gctcccccgg caaagataca atccttcggc gaacgaagct tcacgaatgg
     2161 agtggccatg aaccgatccg cgtctcgcgc gaccgtcgtc ctcgtcctgc tcatcgccct
     2221 cttcggagcc gccggctgca tcgagaaggc gaagccggcg tcgcccgccg ggttcattcc
     2281 ggagaaagag ctcgagaagc cgtcggacct ccccttccag aaggcctggc gaaagaagga
     2341 cctcgactgg caccactaca ccgagatcta cgtcgcgccg tggaacacgg agtacctccg
     2401 ccagatgaac tggtgggaaa agctcgagcg gggggagaag gtcaaagagg atgcgaagga
     2461 gctcgccggg tacgcgcgcg gcgtcttcga ggaggcgttc cgcaacgacc ggaagcatcg
     2521 gttcaccgtc gtcgaacaac ccggaccgaa cacgctcatc gccgagttcg cgctgaccga
     2581 ggtcatcccg aacaagatcg tgctcgacgc gctcggctac gctgcggggc cggcggtcgg
     2641 cgccgccggc acggtggccg cgaacgtgca gaccgcgacg acgcacagca cggtggcctt
     2701 cgaagcgcgg atgaaggacg gggcgacggg ccaggtcgtg gcgatgttcg ctgaccgcga
     2761 ggcggagaag ttttcgctcg tcaacgtcaa ggacctgacg tggtacgggc acgcgaagag
     2821 cattcttcgc gaatgggcgg atcagttcgt gaagatcctg aacaagcagc cgggcgagat
     2881 cgtcaaggac tccgcgccgt tcgacctgag accctggtaa aggtcttggc ggggtcaggt
     2941 cttcacgaat cgcgggaaag actatccagc cgagctggtc ttgccaccat cgcgtagcga
     3001 tgtggcaagt ggagacctga cccccaatgt ggcaagtgga gacctgaccc ctacagcttc
     3061 gcccactcct tccagcggcg ggagagctct tcgggtgtga gcccagtctc gacccgaagg
     3121 gcttcggcga agctccggcc ctcccccagg tcgcgcgtga gacacgcgac cgcgccactc
     3181 ccgtggtgct cgaagaggta ttcgacgacc gcgagcgcct ggacgtagtt cgttcgcgcg
     3241 gcggcccggt ctgcgcgctt cgcgaggttt ccttccatct ccgacagaga gtgcggcgat
     3301 cggccgcgga actcctgccg catctcactc accgggattc gtttcccttc ccaccactgt
     3361 gcgagcccct cgtgcagcca gcccggggcg cggtcggcgg aggcctggcg gacgaacgag
     3421 tgagcgagct cgtgcgcgag aacgaacgtc agctcgggcg tcaccccccc gtcggggtcg
     3481 agcgagacgc ggatctttcc gtcgaacacg cccgacaccc agtccggcac cgagacgagc
     3541 gaaaagtagg agcgccccgc gtagaggatg acgatctgcg ccgttgggag atccgtcccg
     3601 aagaacgccg actgctcgtc gtagatcgct gccagccggt cggcgatttt ttcgaccacc
     3661 ggcgcggaga tcgccgggtc ccagtagaag gagaacctct cgttctcgaa gtgtcgctgc
     3721 ccggacgcga gcgcgagttc ctgccgcagt ttcgagagct gccgcagagt cgtcgggctc
     3781 tcgccgccga tcctttggaa atgttcccaa gcggcgacag catcctcgag acgattcaga
     3841 gagtagtaga cctctccgag gatttcgtac gcctggggag gcgcgcgggg ggcgcgttgc
     3901 tcggcgagat cgagatagaa ccgcgcgaac ggaaagtcgg cgcgccgcac gtacgcgagc
     3961 gtgagcagcg cgagcacgag cccgttttcc tcgtcccagc cgtacgcttc ctcgagcagc
     4021 tcgacggccc tcccggcgtc tccccgctcg agata
//
LOCUS       DASWAV010000074         3178 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U2665
            k141_42730, whole genome shotgun sequence.
ACCESSION   DASWAV010000074 DASWAV010000000
VERSION     DASWAV010000074.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960316
            Sequence Read Archive: SRR10260007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 3178)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3178)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:47:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,929
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3178
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_42730"
                     /isolate="SMAG_U2665"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.36444 N 132.31889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(68..988)
                     /locus_tag="VF958_01670"
     CDS             complement(68..988)
                     /locus_tag="VF958_01670"
                     /inference="COORDINATES: protein motif:HMM:NF016623.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divergent polysaccharide deacetylase family
                     protein"
                     /protein_id="HEX9147846.1"
                     /translation="MEDPPRRRISPATVFFALLALGLGIALYLKTRPSEEAKGPPRAP
                     TARHAARRPKAAPTAAAAPAPEPSPLPAAQARIAIVIDDLGNDADALERIARWPYAVA
                     GAVLPGLPGSAGAARRLAASGKEVLLHLPMEPDGYPLVAPGPGVILRSDTDEAIAHTV
                     IEDLASVPGAVGVNNHMGSAATADPRVMRAVVRVLAERGLFFIDSRTTEATVARRVAD
                     EAALPAVSRRVFLDAVPSASAIERAYRDLLAKAKKDGSALAIGHPHPATLELLERELP
                     RVREEGIALVPVSRLAHSGKTRVKVAALEP"
     gene            complement(995..2149)
                     /locus_tag="VF958_01675"
     CDS             complement(995..2149)
                     /locus_tag="VF958_01675"
                     /inference="COORDINATES: protein motif:HMM:NF015531.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HEX9147847.1"
                     /translation="MRSNRSRLLFLLVSVAALASLRAVDSGAGKDPATKALSVFSEVF
                     ALTRANYVDPTDSKALLEGAYDGMTDALDPYSYYVPAASMSAYRAQQASGALTPGIII
                     ARRGGFPYVVAALPGSPARREGVEPGDLIHSVDGKNLRTAPLWKIRSALEGPEGTTVE
                     VGLFRVVEDKKITLRLRREKFSPPPAETRWERDLAIVKIPALAASSSEALARVIEEAR
                     RRSIDKLVIDLRGSIGGEIADAVPAATLFVGRGPVAKLVTRKVALKPLDATGESIWKG
                     RTIVLIDDSTGGPAEVFAAALKDRAGATTVGETTAGLAIVQRLVPTGSGGNLFMTVAR
                     YQSPSGTILGGKGLSPDERVITFPGDGEGRDLILERGLEVARRTPARQAA"
     gene            complement(2197..>3178)
                     /locus_tag="VF958_01680"
     CDS             complement(2197..>3178)
                     /locus_tag="VF958_01680"
                     /inference="COORDINATES: protein motif:HMM:NF013700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HEX9147848.1"
                     /translation="TLSEELARLELQLEIAAQEKELLARLREDYAARLEAITAERKSA
                     EEGASRSRRELLARARLLQRFGRFGYVRVLLEARDVPAFLESVSRLDSLARRDARLLT
                     EHREAQRRLESGLARERALKTETDRLYAESRREESRIASLKTERENLLERQRAVSASQ
                     RLEVAALSDKAARLEGLLERLSRASAEPVGPGGGIRPWKGVLDWPVRGAVVETFGRHR
                     HPKFDAWTISNGISLGVPAGTPVRAVFAGKAVYAQWLAEYGNLVILDHGDGVFTLYAW
                     LQAVSTRAGTYVPAGTTVGLAGTGPGREEAGLYFEVRDRQKASDPVAWLR"
BASE COUNT          487 a         1103 c         1117 g          471 t
ORIGIN      
        1 cgtgggagac gtcagcccgg cggagctcga cgagtactgg gagcgggcga aggcggagga
       61 gtagacctca aggttcgagg gccgcgacct tgacccgggt cttgccggag tgagcgagcc
      121 gcgagaccgg aaccagcgcg atcccttcct cccggactct cgggagctcc cgttccagca
      181 attcgagcgt cgcgggatgc gggtgcccga tcgcgagagc ggatccgtcc ttcttcgcct
      241 tcgcgagaag atcgcgataa gcacgttcga tcgcagaagc gctcggcacc gcgtcgagga
      301 acacccggcg cgacaccgca ggcagagcgg cttcgtccgc gacccgcctg gccaccgtgg
      361 cttcggtcgt ccggctgtcg atgaagaaaa gtcccctctc cgcgagcacg cgaaccacgg
      421 cccgcatcac ccgcgggtcg gcggtcgcgg ccgagcccat gtggttgttg accccgacgg
      481 cgcctggcac ggacgcgagg tcctcgatca ccgtgtgcgc gatcgcctcg tcggtgtccg
      541 accggagaat gacgcccggg ccgggcgcga ccagcggata accgtccggc tccatcggga
      601 ggtggagcag aacttccttt cccgaggcgg cgagacgccg tgcggcgccg gcgctcccgg
      661 ggagaccggg cagcaccgcg cccgcgacgg catacggcca gcgcgcgatc cgctcgagcg
      721 cgtcggcgtc gtttccgagg tcgtcgatca cgatcgcgat ccgcgcctgc gccgccggga
      781 gaggcgacgg ctcgggagcg ggggctgccg ccgccgtggg agccgccttc ggccttcggg
      841 ccgcgtgccg cgccgtcggg gcgcggggag ggcctttcgc ttcctcagac ggacgggtct
      901 tgaggtaaag cgcgatgccg aggccgagtg cgaggagcgc gaagaagacc gtcgccgggg
      961 agatgcggcg gcggggaggg tcttccaatc ggctttatgc ggcctggcgg gcgggagtgc
     1021 gacgcgccac ttcgaggccg cgttcgagga tgaggtcgcg gccttcgccg tcgcccggga
     1081 acgtgatcac ccgttcgtcc ggcgagagac ccttgccgcc gagaatcgtg cccgacggtg
     1141 actggtaacg cgcgaccgtc atgaacaggt ttccgccgga accggtcggc acgagacgct
     1201 ggacgatcgc cagtcccgcg gtcgtttccc cgaccgtcgt cgcgcccgca cggtccttga
     1261 gcgccgcggc gaacacctcg gcgggacctc cggtcgagtc gtcgatcagg acgatcgtgc
     1321 gccccttcca gatgctctcg ccagttgcat cgagcggctt cagggcgacc ttgcgggtca
     1381 cgagcttcgc gaccggaccg cggcccacga agagcgtcgc cgcaggaact gcgtcggcga
     1441 tctccccgcc gatcgatccc cgaagatcga tcacgagctt gtcgatcgac cgccgcctgg
     1501 cctcctcgat gacccgcgcg agagcttcgc tcgagctggc ggccagcgcc gggatcttca
     1561 cgattgcgag gtccctttcc cagcgtgtct cggccggggg cggcgagaac ttctcgcgcc
     1621 gcaggcgcag cgtgatcttc ttgtcctcga cgacgcgaaa gagcccgacc tccacggtcg
     1681 tgccctcggg gccttcgagc gcggaccgga tcttccagag aggcgctgtg cggaggttct
     1741 tgccgtcgac cgaatgaatc aggtcgcccg gctccacgcc ctcccgccgg gccggcgatc
     1801 cgggaagcgc cgcgaccacg tagggaaagc ccccgcgccg ggcgatgatg attccgggcg
     1861 tgagcgcgcc ggacgcctgc tgggcccggt acgccgacat cgacgcggcc gggacgtagt
     1921 aggagtaggg atcgagggcg tccgtcatcc cgtcgtaggc gccttcgaga agcgctttcg
     1981 agtcggtcgg gtcgacgtag ttggcgcgcg tgagggcgaa gacctcggaa aagacggaga
     2041 gcgctttcgt cgcggggtct ttgccggccc cggaatcgac ggctcgcaga gaggcgagcg
     2101 cggcgacaga aacgagcagg aagagcagtc gggaccggtt cgatctcatc gtgcggatgg
     2161 taacacccgg agaaacgggc ttcttccgat cggttttcac ctcagccatg cgaccggatc
     2221 gctcgccttc tggcgatctc tcacctcgaa gtacagcccc gcctcctctc ttccgggacc
     2281 cgtcccggcg agccccaccg tcgtcccggc gggaacgtac gttccggcgc gggtcgacac
     2341 ggcctgcagc cacgcataga gcgtgaagac cccatcgccg tggtccagga tcacgaggtt
     2401 cccgtactcc gcgagccact gcgcgtagac ggccttgccg gcgaacacgg ctcggaccgg
     2461 cgtccccgcg ggcaccccga gcgagatccc gttcgagatc gtccaggcgt cgaatttcgg
     2521 atgacgatgg cggccgaacg tctcgacgac cgcgccgcgc acgggccagt cgagaacgcc
     2581 tttccacggc cggatccccc cgcccggccc gaccggctcc gccgaggcac gcgagagccg
     2641 ctcgaggaga ccctcgagac gcgcggcctt gtcggagagt gccgcaacct ccaggcgctg
     2701 cgaggccgag acggcgcgct gccgctcgag caggttctcg cgctccgtct tcaacgaagc
     2761 gatgcggctt tcctcgcgac ggctctcggc gtagagacgg tcggtctcgg tcttcaaagc
     2821 cctctccctc gcgagcccgg attccaggcg tctctgcgct tcgcgatgct ccgtgagaag
     2881 ccgggcatcg cggcgggcga gcgagtcgag ccgggagacg ctctccagga aagcggggac
     2941 gtcccgcgcc tccaggagca cgcgcacgta cccgaaacgg ccgaagcgct ggagaagccg
     3001 cgcgcgggcg agcagttccc ggcggctccg agaggccccc tcttccgccg acttgcgctc
     3061 ggcggtgatc gcttcgaggc gggcggcgta gtcttcgcgc agccgcgcca gcagttcctt
     3121 ctcctgagcc gcgatctcga gctggagctc aagacgggcg agctcttcgg aaagcgtc
//
LOCUS       DASWAV010000075         4190 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U2665
            k141_282859, whole genome shotgun sequence.
ACCESSION   DASWAV010000075 DASWAV010000000
VERSION     DASWAV010000075.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960316
            Sequence Read Archive: SRR10260007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 4190)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4190)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:47:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,929
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4190
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_282859"
                     /isolate="SMAG_U2665"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.36444 N 132.31889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1665
                     /gene="metG"
                     /locus_tag="VF958_01685"
     CDS             <1..1665
                     /gene="metG"
                     /locus_tag="VF958_01685"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: protein motif:HMM:NF008900.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HEX9147849.1"
                     /translation="FGIANDDFIRTTEPRHRRGVEELVRRIEASGDLYLDRHEGWYCT
                     SCEAFYTEKELVEGKCPVHERPAAWQSEENVFFRLSRYAAPLLEIYRTRPEFVRPENR
                     RAEVMSFVEAGLKDISVSRTNVKWGVPFPGRPGHVVYVWLDALSNYVTALGFGSEDDR
                     LYREFWDNAHGRRVHLIGKDILRFHAVYWPAFLLSAGLPLPTSVWAHGWWLRDEKKMS
                     KSIGNVVRPDALVSDFGPDALRYFLMREMAFGQDASFSDQAFLERFNADLANDLGNTV
                     SRVSALCRQAFGSSPPEACEDNEVIAAWGRARRDYGGAMDDFAFHRGLEAIWRFLTEI
                     NGYVVSREPWKVRKEEGPSARLSRILYAASEGVRLAACLLWPFMPGTSRKIFAALGAP
                     ADDPRLADLEWGRLPTTAPISTAEALFPRVDTAEYFSKKEGSAMTDIPPPESDGRISI
                     QDFQRVELVTGRVLEAERVPKSNKLVRLQVDLGSERRQVVAGIAARYEPETLVGRNVV
                     IVANLKPAKLMGVESNGMVLAATVGEAGEPSLLEVPADVPPGSKVK"
     gene            1691..2479
                     /locus_tag="VF958_01690"
     CDS             1691..2479
                     /locus_tag="VF958_01690"
                     /inference="COORDINATES: protein motif:HMM:TIGR00010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HEX9147850.1"
                     /translation="MTVLKAILADSHCHLQMGGGATAELLARARAAGVARFLVPGTTL
                     ADSSASLAIASDHEDVLAAVGVHPHEAKDFDFDRDGVALEDLAGKPEVAAIGEVGLDF
                     HYDFSPRGKQIAVLEWMLDLARRRDLPVVLHNRESGAELLSLLTRLGPRENAGVFHSF
                     TETPDYGRKVIDLGFRISFSGMITFAGAQNIRGAALALPLDAMLVETDSPYLSPVPHR
                     GKPCEPAFVVETARKLAEVQGIPFERVAEVTTGNYERLFFRRLA"
     gene            2476..2847
                     /locus_tag="VF958_01695"
     CDS             2476..2847
                     /locus_tag="VF958_01695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallopeptidase family protein"
                     /protein_id="HEX9147851.1"
                     /translation="MTSPGRISPREFEAIVEEALGGVPEAFRRYLDNVVVVVEEEPSH
                     EDYGETDTPDDEELFGIFRGASFFERDRAVTNLPAQIAIFRGPILRSCATRGEAVREI
                     RDTVVHELGHLLGLDDEEMPY"
     gene            complement(2941..3765)
                     /locus_tag="VF958_01700"
     CDS             complement(2941..3765)
                     /locus_tag="VF958_01700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9147852.1"
                     /translation="MRTRKLETLSLAVLAGALVIGSAACQKRVETVVVVTPTVGPAPA
                     AAYTPLPGLQPADTSTITADPIVVSQRNAVKRRTATQSRKTSSASASSTREGAPAEPP
                     RAAKEIPQGTILRVAFDEAISSATAKVGDTVRGKLLDDVLADDGSVVAASGSGVSGKI
                     EHVVDSGKLARPAELRFRLTDLETRSGTVPIQTSAYDRVGDTHTKRNVEYIAGGAAVG
                     AIVGQILGKDTGSTLKGAAAGAAVGTGVAAATGDLDFTIDAGRTVAFTLEQPVHIR"
     gene            complement(3847..>4190)
                     /locus_tag="VF958_01705"
     CDS             complement(3847..>4190)
                     /locus_tag="VF958_01705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012447907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HEX9147853.1"
                     /translation="VEPYGAFVEILPGQDGLVHISELAPYRVRQTTDVVKEGDEVTVK
                     IIAVDPTGKIKLSRKQALSKEELETEMAMAPVGPGEEVGAREAERPREGDHFGHPNRD
                     PRDRGPRGHRK"
BASE COUNT          639 a         1413 c         1426 g          712 t
ORIGIN      
        1 ttcgggatcg ccaacgacga cttcatccgg acgacggaac cgcggcaccg ccgcggcgtc
       61 gaggaactcg tgcgccgaat cgaagcgtcc ggcgaccttt acctcgaccg ccacgagggg
      121 tggtactgca cgagctgcga ggcgttctat acggagaagg agctcgtgga gggaaagtgc
      181 ccggtgcacg agcggcccgc ggcctggcaa tcggaggaga acgtcttctt ccgcctgtcg
      241 aggtacgcgg cgccgctcct cgagatctac cggacgcggc cggagttcgt ccgccccgag
      301 aaccggcgcg cggaagtgat gtcgttcgtc gaagccggcc tgaaggacat ctcggtgtcg
      361 cggacgaacg tcaagtgggg agtgccgttc ccgggtcggc cgggccacgt cgtttacgtg
      421 tggttggacg cgctctcgaa ctacgtcacg gcgcttggct tcggatcgga ggacgaccgg
      481 ctctaccggg agttctggga caacgcccac gggcgccgcg ttcatctcat cggaaaagac
      541 atcctccggt tccacgccgt ctactggccg gcgttcctgc tgtcggccgg gcttcccctg
      601 ccgacgagcg tctgggccca cggctggtgg ctgcgcgacg agaagaagat gtccaagtcc
      661 atcggaaacg tcgtgcgtcc cgacgcgctc gtctccgact tcggccccga cgccttgcgt
      721 tacttcctca tgcgggagat ggccttcggc caggacgcgt ccttctcgga ccaggcgttt
      781 ctcgagcgct tcaacgcgga cctcgcgaac gacctcggaa acaccgtctc ccgcgtgtcc
      841 gcgctctgcc gtcaggcgtt cggttcctcg ccgcccgaag cgtgcgagga caacgaggtc
      901 atcgcggcat gggggagagc ccgccgggac tacggcgggg cgatggacga cttcgccttc
      961 caccgcgggc tcgaggcgat ctggagattc ctgacggaaa tcaacgggta cgtcgtgtcg
     1021 cgcgagccgt ggaaggtccg gaaggaggag gggccgtcgg cgcggctctc gcgcatcctc
     1081 tatgccgcgt ccgagggggt acgccttgcg gcgtgcctgc tgtggccctt catgccgggg
     1141 acgtcgcgaa agatcttcgc cgcgctcgga gcgccggccg atgaccccag gctcgcggac
     1201 ctcgagtggg gacggctgcc gacgacggct cccatctcga cggcggaggc gctctttccg
     1261 agagtggaca cggccgagta cttttcgaag aaggagggga gcgcgatgac ggacatcccg
     1321 ccgccggagt cggacggccg catttcgatc caggatttcc agcgagtcga gctcgtgacc
     1381 gggcgcgtgc tcgaggccga gcgcgtgccg aaatcgaaca agctcgtgcg gctgcaggtc
     1441 gacctcggca gcgaacggcg ccaggtcgtc gccggtatcg ccgcgcgcta cgagccggag
     1501 accctcgtcg gcaggaacgt cgtcatcgtc gcgaacctga agccggcgaa gctgatgggg
     1561 gtcgaatcga acgggatggt gctggcggcg accgtcgggg aggctggaga gccctcgttg
     1621 ctggaggtcc ctgccgatgt cccgcccggg agcaaggtca aatagattcc tcgtcgcttc
     1681 gctcctcgga atgacggtat tgaaagcgat cctcgccgac tcgcactgcc acctgcagat
     1741 gggaggcggt gccaccgccg aactgctcgc gcgcgcgcgc gccgctggtg tcgcgcgttt
     1801 cctggtgccg ggcacgacgc tggcggactc ctcggcaagc ctcgcgatcg cgtcggacca
     1861 cgaggacgtc ctcgctgccg tcggcgtcca cccgcacgag gcgaaagact tcgattttga
     1921 ccgcgacggc gtcgcgctcg aagacctcgc gggaaagccc gaggtggccg cgattggaga
     1981 ggtcgggctc gactttcact acgatttctc tccgcgcggc aagcagatcg cggtgctcga
     2041 gtggatgctc gacctcgcac gccgccgcga cctgccggtc gttctccaca accgcgagag
     2101 cggcgctgag ctcctgtcac tgctcacgcg gctcgggccc cgggagaacg ccggcgtctt
     2161 ccattccttc acggaaacgc ccgactacgg gcgcaaggtc atcgacctcg gcttccgcat
     2221 ctcgttttcc ggaatgatca cgttcgcggg cgcgcagaac atccgcggcg cggctctcgc
     2281 gctcccactc gacgcgatgc tcgtcgagac cgactctcct tatctctcac ccgtgccgca
     2341 ccggggcaaa ccctgcgagc cggcgttcgt cgtggagacg gcgaggaaac tcgcagaggt
     2401 gcagggcatt ccgttcgagc gcgtcgccga agtgacgacg ggcaactacg aacggctctt
     2461 cttccgccga ctcgcgtgac gagccccggc cgcatctccc ctcgcgaatt cgaagcgatc
     2521 gtcgaagagg cgctcggcgg ggtgccggag gcgtttcgcc ggtacctcga caacgtggtc
     2581 gtcgtcgtcg aggaggagcc gtcccacgag gactacggcg agaccgacac tcccgacgat
     2641 gaggagctgt tcggaatctt ccgcggcgcc tcgttcttcg agcgggaccg ggccgtcacg
     2701 aacctgccgg cgcagatcgc gatcttccgc ggcccgatcc tgcgctcgtg cgcgactcgc
     2761 ggcgaagccg tgcgcgagat ccgcgacacc gtcgtccacg agctggggca tctcctgggc
     2821 ctcgacgacg aggagatgcc ctactagacg ggaatcggga actccggaat cgacttgcga
     2881 ttcttttcga aaacagacca tccgggtggg tggtcttgag agagcccgac tcgaaacgcg
     2941 ctatcggatg tgcaccggct gctcgagcgt gaacgccacg gtgcgtccgg cgtcgatcgt
     3001 gaagtcgagg tccccggtcg ccgcagcgac tccggttccg accgccgctc cggcggccgc
     3061 tcccttcaac gtcgaccccg tgtccttgcc gagaatctgg ccgacgatcg cgccgacggc
     3121 cgcgccgccc gcgatgtatt cgacattgcg cttcgtgtgc gtgtcgccga cgcggtcgta
     3181 tgccgacgtc tggatcggga ccgttccgct ccgggtctcg aggtccgtca gccggaaccg
     3241 cagctccgcg ggccgagcga gcttgcccga gtccacgacg tgctcgatct ttccggagac
     3301 accgctgccg gaggccgcga cgacgctgcc gtcgtcggcg aggacgtcgt cgaggagctt
     3361 tccgcgcacg gtgtcgccga ccttcgcggt cgccgacgag atcgcctcgt caaaggccac
     3421 gcggaggatc gtcccctgcg ggatttcttt cgcggcgcgg ggcggctcgg cgggcgcgcc
     3481 ctcgcgcgtc gaactcgccg acgccgagga ggtcttccgc gactgggtgg cggtgcgccg
     3541 tttcacggcg ttcctctgcg aaacgacgat cggatctgca gtgatcgtgc tcgtgtcggc
     3601 cggctggagt cccgggagcg gcgtgtaggc ggcagccggc gcgggcccga cggtcggggt
     3661 gacgacgacg accgtctcca ctcttttctg gcacgcggcg gacccgatca ccagagcccc
     3721 ggcgaggacc gccagcgaca atgtttcgag tttccgggtt cgcatctcag agcacctcct
     3781 tgtcatcggc ggttggttga gcaggaaccg tgccccggat gggcgcctgg agcaaccgca
     3841 caagactcac ttgcgatgcc cgcggggacc ccggtcgcga gggtcccggt tcgggtgccc
     3901 gaagtggtca ccctctcgcg gacgctcggc ctctctcgcc ccgacctcct ctcccggtcc
     3961 cacgggcgcc atcgccatct ccgtctcgag ctcctctttc gagagagcct gtttgcggga
     4021 gagcttgatc ttgccagtgg ggtcgacggc gatgatcttg actgtgacct cgtcgccctc
     4081 cttgaccacg tcggtcgtct ggcggacgcg gtacggagcg agctccgaga tatggacgag
     4141 gccgtcctgt cccggcagga tctcgacgaa ggcgccgtac ggctcgaccc
//
LOCUS       DASWAV010000076         3197 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U2665
            k141_124046, whole genome shotgun sequence.
ACCESSION   DASWAV010000076 DASWAV010000000
VERSION     DASWAV010000076.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960316
            Sequence Read Archive: SRR10260007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 3197)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3197)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:47:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,929
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3197
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_124046"
                     /isolate="SMAG_U2665"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.36444 N 132.31889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..217)
                     /locus_tag="VF958_01710"
     CDS             complement(<1..217)
                     /locus_tag="VF958_01710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010393930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HEX9147854.1"
                     /translation="MPALLVTAFLIGLAGGVHCMAMCGGIVAALNLHAQATPLPSRGS
                     RFGRQLAYSLGRISSYAFAGAIAGAAGG"
     gene            261..407
                     /locus_tag="VF958_01715"
     CDS             261..407
                     /locus_tag="VF958_01715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9147855.1"
                     /translation="MPPTAEEKIRMAEVPKGTLALMTIVAILLFAGWALLFFGRFLGS
                     GPVR"
     gene            407..925
                     /locus_tag="VF958_01720"
     CDS             407..925
                     /locus_tag="VF958_01720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010961623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome C oxidase subunit II"
                     /protein_id="HEX9147856.1"
                     /translation="MHVHPLERRWAWIAALLLAALIVLVSYTAVVMNIHPPSNVETID
                     STQLHLTPEFAEDNLGVKLSREGTVVAHLVMARFSVHPQAITLPAGTPIVLRAASADV
                     LHGLHVPGTNIGTQVVPGYVSEVHTTIDFDRVAKVGVANPDGSVTVPLYCNEYCGLGH
                     QSMWGRVRVTRK"
     gene            922..2553
                     /locus_tag="VF958_01725"
     CDS             922..2553
                     /locus_tag="VF958_01725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020564654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0009060 - aerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase subunit I"
                     /protein_id="HEX9147857.1"
                     /translation="MSSVAPPNRPDRLSIAHMWVGFGLFGVGVLLGLYQLLERNGVLA
                     TSAAGYYGSATLHGVIQAFVLTTFFIVGFGYFVTSTSLRTSIRWPRLAWIGFATMFGG
                     TLIAAITIAAGKATVLYTFYPPMVAHWAFYVGAALLLVGSIPWLVITVAMTMRWRREN
                     PGRTIPLPQFGMTACAILWAWTLIGVVAEVVFQLLPLALGLTQTIDIGLARVLFAWTL
                     HPIVYFWLIPAYVALYTIVPKAAGGYLFSDEMARVVFVMLLVFSLPVGLHHIFVDPEQ
                     AAGWKLMHAFATFLVALPTLLTGFTVAASLETAGRLRGGGGLFGWIRALPWGEPVVAG
                     GILAALLLLPGGFGGLVNASYAMDTMVHNTMWIPAHFHLIFGGSVVIVYMASAYALWP
                     KLTGRRLPSRRMATAQLWLWFLGILALTIPWHLVGLEGMPRRTAYNPYDPQIVERWRP
                     YTGAMIVGGVVLTISGLMFLLNLLLVHRSREPEQDRDMSYAETIEPVVTVPALLNGFA
                     IWNWLLLVLMVLAFAYPIGQFFFIDVHKALAWGAR"
     gene            2550..>3197
                     /locus_tag="VF958_01730"
     CDS             2550..>3197
                     /locus_tag="VF958_01730"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HEX9147858.1"
                     /translation="MIPRRPAVLVVVALLAAAATLVSAPSSRVAWTLETVQRARSGDV
                     ARGKKLHEDCAACHGDAGNVDTPDVPDLGGQNPLYTYKQLVDFKTETRASSIMNDAAK
                     ALSERDIADLAAFYAGQGPPKPAAAPNAPEAAIAKLVSIGDGVRLVPACDACHGDRGA
                     GNPQFYGIPVLKNQKLQDIVVQLTAYRSGERANDVYSVMRELCKKLSDAEIAGLAA"
BASE COUNT          497 a         1096 c         1018 g          586 t
ORIGIN      
        1 gccccccggc ggcgcccgcg atcgcacccg cgaatgcata gctcgagatg cggccgaggc
       61 tgtacgcgag ttgccggccg aatctcgaac cgcgtgacgg gagcggcgtc gcctgcgcgt
      121 ggagattcaa cgccgcgacg atccccccgc acatggccat gcagtgaacc cctcccgcga
      181 gaccgatcag gaacgcagtg acgagaagcg cgggcatccg cggacggatt atgatcccgc
      241 caacaaaccc ggagaccgac atgccaccga ccgcggaaga aaagatccga atggccgagg
      301 tgccgaaggg aaccctcgcg ctcatgacta tcgtcgccat ccttctcttc gcggggtggg
      361 cgcttctctt tttcggccga ttcctgggta gcgggccggt gcgctgatgc acgtccaccc
      421 gctcgagcgc cgctgggcct ggatcgcggc gctgcttctg gcggccctga tcgtgctcgt
      481 ctcctacacc gcggtggtca tgaacattca tccgccgagc aacgtcgaga cgatcgactc
      541 gacgcagctt cacctcaccc cggaattcgc cgaggacaac ctgggtgtca aactttcgag
      601 ggaggggacg gtcgtcgcac atctcgtcat ggcacggttc agcgttcacc cgcaggcgat
      661 caccctgccc gcgggaacgc cgatcgtgct gcgcgcggcg agcgccgacg ttctccatgg
      721 cctccacgtt cccggcacga acatcggcac ccaggtcgtg ccgggatacg tctccgaggt
      781 ccacacgacg atcgacttcg atcgcgtcgc gaaggtcggc gtcgcgaacc ccgacggcag
      841 cgtcacggtt ccgctctact gcaacgaata ctgcggtctc ggacaccagt cgatgtgggg
      901 ccgcgtccgc gtcacgcgca aatgagctcc gtcgcgcctc cgaatcgccc cgatcgcctc
      961 tcgatcgccc acatgtgggt ggggttcggg ctcttcgggg ttggagtcct tctcgggctg
     1021 taccaacttc tcgaacgcaa tggagtcctc gctacctccg ccgccggcta ttacggatcg
     1081 gcgacgctcc atggagtcat ccaggcattc gtcctcacga cgttcttcat cgtcggcttc
     1141 ggatacttcg tcacgtcgac gagtcttcgg acctcgatcc ggtggccgcg cctcgcctgg
     1201 atcgggttcg caacgatgtt cgggggcacc ctcatcgcgg cgatcacgat cgccgcgggc
     1261 aaagcgacgg tgctctacac gttctacccg cccatggtcg cgcactgggc cttctacgtg
     1321 ggagccgcgc tgctcctggt cggatcgata ccgtggctcg tcattaccgt ggccatgacc
     1381 atgcggtgga ggcgcgagaa ccccggacgc acgatccccc tcccgcagtt cgggatgacg
     1441 gcgtgtgcca tcctatgggc ctggacgctg atcggcgtcg tcgccgaagt ggtgtttcag
     1501 ctcctgccgc tcgcgctcgg tctcacgcag acgatcgata tcgggctcgc ccgagtgctc
     1561 ttcgcctgga ctctccaccc gatcgtctac ttctggctga ttccggcgta cgtcgcgctc
     1621 tacacgatcg ttccgaaggc ggcgggcgga taccttttct cggatgagat ggcgcgcgtc
     1681 gtgttcgtga tgctcctggt cttcagcctg ccggtcggtc tccaccacat cttcgtcgac
     1741 ccggagcagg ccgccggctg gaagctgatg cacgcgttcg cgactttcct ggtcgcgctt
     1801 ccgacgctgc tgacgggatt cacggtggcc gcctcgctcg aaacggcggg acgcctgcgc
     1861 ggcggaggcg gcctcttcgg atggatccgc gcccttccct ggggcgagcc ggtcgtcgcg
     1921 gggggaatcc tcgcagcgtt gctgctcctg cccgggggtt tcggcggcct ggtcaacgcg
     1981 tcctacgcga tggacaccat ggtccacaac acgatgtgga taccggcgca cttccacctc
     2041 attttcggcg gctccgtcgt catcgtttac atggcgagcg cctacgcgct ctggccgaag
     2101 ctgaccggac gacggcttcc ctcgcgccgg atggcgaccg cgcagctgtg gctgtggttc
     2161 ctcgggattc tcgcgctgac gattccgtgg cacctcgtgg gcctcgaggg gatgccgcgc
     2221 cgcacggcgt acaacccgta cgacccgcag atcgtcgagc gctggcgtcc ctacacgggc
     2281 gcgatgatcg ttggcggtgt ggtcctcacc atttcgggac tgatgtttct cctcaatctc
     2341 ctgctcgtcc accgcagccg cgagcccgag caagaccgcg acatgagcta cgcggaaacg
     2401 atcgaaccgg tcgtgacggt ccctgctctc ctgaacggtt ttgcgatctg gaactggctg
     2461 ctgctcgttc tcatggtgct cgccttcgcg tacccgatcg ggcaattctt cttcatcgac
     2521 gtgcacaagg ctctcgcgtg gggggcgcga tgatcccccg gcgaccggcg gttctcgtag
     2581 tcgtggcgct gctcgcggcg gcagcgacgc tcgtgagcgc gccttcgtcg cgggtcgcat
     2641 ggaccctcga gaccgttcag cgcgcgcgga gcggcgacgt cgcgcggggc aagaagctcc
     2701 acgaggactg cgccgcctgc cacggcgacg cgggaaacgt cgacaccccg gacgttccgg
     2761 acctcggagg ccagaatccg ctctatacgt acaagcaact cgtcgatttc aagacggaga
     2821 cgcgggcgag ctcgatcatg aacgacgcgg cgaaggcgct ctccgagcgc gacatcgcgg
     2881 acctcgccgc gttctatgcg ggccagggcc cgccgaagcc ggccgcggcc ccgaacgcgc
     2941 cggaggctgc catcgcaaag ctcgtttcga tcggagacgg cgtgcgtctc gttcccgcgt
     3001 gcgacgcctg tcacggagat cgcggcgccg gcaacccgca gttctatgga atcccggtcc
     3061 tgaaaaacca gaagctccag gacatcgtcg tgcagctcac ggcgtaccgg tcgggagagc
     3121 gcgccaacga cgtctacagc gtgatgcggg agctgtgcaa gaagctttcc gatgcggaga
     3181 tcgccggtct cgccgcg
//
LOCUS       DASWAV010000077         2841 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U2665
            k141_323708, whole genome shotgun sequence.
ACCESSION   DASWAV010000077 DASWAV010000000
VERSION     DASWAV010000077.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960316
            Sequence Read Archive: SRR10260007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2841)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2841)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:47:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,929
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2841
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_323708"
                     /isolate="SMAG_U2665"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.36444 N 132.31889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(146..694)
                     /locus_tag="VF958_01735"
     CDS             complement(146..694)
                     /locus_tag="VF958_01735"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEX9147859.1"
                     /translation="MTAGRREALLQALRAYRGENPRDEGTTSRFRAFLERDDPFRRSD
                     PDGHVTASAVVARPSGDAFLLVFHRKLDRWLQPGGHVEDGDTSVFHVALREAREETGI
                     SDFEAPLGDAIFDLDVHAIPAFGDDPAHFHYDVRFLLTVGDGEKPEPGAAWFPFRAVG
                     RTDTDGSLVRAVLKAIKRLALQ"
     gene            873..2093
                     /locus_tag="VF958_01740"
     CDS             873..2093
                     /locus_tag="VF958_01740"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HEX9147860.1"
                     /translation="MTRKKKRWIVLGVLGVIVAGGAIVAMRGKDKPKGDAELPFRLGK
                     VQAEDLQVSVREVGVVDPVTKVDVKSPVSGRVVGLRVREGAVVKVGDVLAEIEPDVNQ
                     AQSLSDVNAGVTEGKLKLQDAERELTSQKALFDSGLIGQDAMKIAQNKHDIAAESLRA
                     AQMRYQIVQDRGIPISGNAASQKARVTSPMNGVVIKRGVELGQTVTSGVSSFNEGTAL
                     FTVADLKSLIIRVNLNEVDIAKVDVGQPVRITLDAYPQKVFTGKVSFVSPAAELVEKI
                     KVFKVEISLDELGAHFKTGMSANVEILGTKRDKAVSIPLEALQRREGETIAYRLKANL
                     KPQQIKAARDGLSGRNKFIWLSDHWKDYYDVVPVKAGVATLERVEILAGLKPGEQVSL
                     EDPTKKKIEKDEDD"
     gene            2094..2786
                     /locus_tag="VF958_01745"
     CDS             2094..2786
                     /locus_tag="VF958_01745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005521128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX9147861.1"
                     /translation="MARAVIETKALTKTFGSNGVAVHALRGIDLTVDRGEFLALIGPS
                     GSGKSTLMAILGCLDKPTSGSYEFDGERMEGLSNAELARIRNHKIGFVFQMYNLLPKA
                     SVLRNVELPMLYAGVRRKERRRRATELLERVGIPEKAKSLPSALSGGQRQRVAIARAL
                     ANQPAMLLADEPTGALDSKTGHEVLELFSELHHQGNTILIVTHDLSIAALASRQVEIH
                     DGRVAAETSQVA"
BASE COUNT          524 a          940 c          953 g          424 t
ORIGIN      
        1 gcgccgtagg cgaggagccg gactcgcagg ggcgcgtcaa ctccaaacgt cgagaggaag
       61 acgatcgcga taccgaccat cgccgagacg agcgcgcgcc gcgccgtttt cgccaggctc
      121 ggaccgggga ggcggagtgt ctccgtcact gcagagccag tctcttgatc gccttgagca
      181 ccgcgcgcac gagggagccg tcggtgtctg tccgaccgac cgcgcgaaaa gggaaccagg
      241 ccgcgccggg ctccggcttt tctccatccc ccaccgtcag caggaaccgg acgtcgtagt
      301 gaaagtgcgc ggggtcgtcg ccgaatgccg ggatcgcgtg gacgtcgagg tcgaagatcg
      361 cgtcgccgag cggggcctcg aaatcggaaa tcccggtctc ctcgcgcgcc tcgcgcagcg
      421 ccacgtggaa cacggaggtg tctccgtcct cgacgtggcc gcccggctgg agccatcggt
      481 cgagcttccg gtgaaacacg aggagaaacg cgtcgcccga tggtcgcgcg actacggcgc
      541 tcgcggtcac gtgaccgtcc ggatcggacc ggcgaaaggg atcgtcacgc tcgagaaagg
      601 cgcggaagcg ggaggtcgtg ccttcgtccc gcgggttctc gccgcggtac gccctgagcg
      661 cttgcaggag agcctcccgc cgacccgcgg tcatctccct ccggattatg atgggggtca
      721 ggtctccact tgccacattc tctcgtatct gcctgtaaag aaatgacttc ctggatccct
      781 gtcgcttcgc gcgccccggc gtcaccgccg cgacttcccg gaacattccc gcgagggtcg
      841 cgtatctgag tgcagccaac cgggaggctc ggatgaccag gaagaagaag cgctggatcg
      901 tactcggcgt cctcggcgtg atcgtcgccg ggggggcaat cgtcgccatg cgcggcaaag
      961 acaagcccaa gggggacgca gaactcccct tccgcctggg caaggtccag gccgaggacc
     1021 ttcaggtctc cgtccgggag gtcggcgtcg tcgacccggt cacgaaggtc gacgtgaagt
     1081 cgccggtctc cggccgcgtg gtcggcctgc gcgtccgcga gggcgcggtc gtcaaagtcg
     1141 gcgacgtgct cgcggagatt gagccggacg tcaaccaggc gcagtcgctc tccgacgtca
     1201 acgctggcgt caccgaaggg aagctgaagc tccaggacgc ggaacgtgag ctcacgtcgc
     1261 agaaggcgct cttcgactcc ggactgatcg gccaggacgc catgaagatc gcccagaaca
     1321 agcacgacat cgcggcggag agcctgcgcg ccgcgcaaat gcgctaccag atcgtccagg
     1381 accgcggcat cccgatctcg ggcaatgccg cgtcgcagaa ggcgcgcgtc acgagcccga
     1441 tgaacggcgt cgtcatcaag cgcggcgtcg agctcgggca gacggtgacc tccggcgtct
     1501 cctcgttcaa cgaggggacg gcgctcttca cggtcgcgga cctgaaatcc ctcatcatcc
     1561 gcgtcaacct gaacgaggtc gacatcgcga aggtggatgt cggccagccc gtccgcatca
     1621 ctctcgacgc ctacccgcag aaggtcttca ccggaaaagt gagcttcgtc tctcccgccg
     1681 cggagctggt cgagaagatc aaggtcttca aagtcgagat ctcgctcgac gagctcggcg
     1741 cgcacttcaa gaccgggatg agcgcgaacg tcgagatcct cgggacgaag cgcgacaagg
     1801 ccgtgtcgat tccgctcgag gcgttgcagc gacgcgaggg cgagacgatc gcctaccgtc
     1861 tcaaggcgaa cctgaagccg cagcagatca aggccgcccg cgacgggctt tccggccgca
     1921 acaagttcat ctggctgtcc gaccactgga aggattacta cgacgtggtg ccggtaaagg
     1981 cgggcgttgc gacgctcgag cgcgtcgaga tcctcgccgg gttgaagccg ggcgagcagg
     2041 tgtcactcga ggacccgacg aagaagaaga tcgagaagga cgaggacgac tagatggcgc
     2101 gcgcggtcat cgaaacgaaa gccctgacga agacgttcgg cagcaacggc gtcgcggtcc
     2161 acgcgctgcg cggcatcgac ctgaccgtcg accgcggcga attcctcgcg ctgatcgggc
     2221 cgtcgggatc cgggaagtcc accctcatgg cgatcctcgg ctgcctcgac aagccgacga
     2281 gcggcagcta cgagttcgac ggagagcgaa tggaaggcct gtcgaacgcc gagctcgctc
     2341 ggatccggaa tcacaagatc ggcttcgtct tccagatgta caacctgctg ccgaaagcgt
     2401 cggtgctgcg caacgtcgag ctgccgatgc tctacgccgg cgtccggcga aaggagcgcc
     2461 gtcgccgcgc gaccgagctc ctcgagcgcg tcggcatccc ggagaaggcg aagtccttgc
     2521 cctcggccct gtccggcggt cagaggcagc gcgtcgcgat cgcgcgggcg ctcgcgaacc
     2581 agccggcgat gctccttgcc gacgagccga ccggcgcgct cgactcgaag accgggcatg
     2641 aggtcctgga gctcttctcg gagctccacc accagggcaa cacgatcctg atcgtcacgc
     2701 acgacctctc gatcgccgcg ctcgcgagcc gccaggtcga gatccacgac gggagagtcg
     2761 ccgccgagac cagccaggtc gcctgatgtt ctccggcgcc tttcgcgaac gggtccggaa
     2821 ctcgtgggtc gagatgcgcg a
//
LOCUS       DASWAV010000078         1820 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U2665
            k141_763555, whole genome shotgun sequence.
ACCESSION   DASWAV010000078 DASWAV010000000
VERSION     DASWAV010000078.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960316
            Sequence Read Archive: SRR10260007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 1820)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1820)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:47:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,929
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1820
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_763555"
                     /isolate="SMAG_U2665"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.36444 N 132.31889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(155..1471)
                     /locus_tag="VF958_01750"
     CDS             complement(155..1471)
                     /locus_tag="VF958_01750"
                     /inference="COORDINATES: protein motif:HMM:NF013594.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HEX9147862.1"
                     /translation="MRRRFLVVLGALTIPAALLAAPPPAPQPTANPTVGPAPAAYTLP
                     PDKYRQAVAFSRAKYRLHFLGFAWEVLLLGGLIALRVAPRYRDLAERLSRRRFVQALV
                     FVPLLLLTEQVLDLPLAVWGHRLSVFYGQSIQAWDSWLWDWLKGVLIALALFVPVVWV
                     LYGILRRSPRRWWLWFWLATLPIIVFVVFIAPVVVDPLFFDFQPLAGKAPQLVERIES
                     VTERGGLSIPRERMFLMDASRKLKSLNAYVTGFGGSKRVVVWDTTLDRMTIPQTLFVF
                     GHEMGHYVLAHIPKTIAFVAALSLVLLFLAHGVLRRVLPEGREYRGIRGVTDWASLPA
                     LMLVVTVFGEIASPVVASYSRMQEHAADIYGLEVIHGLVPDSQRTAAEAFQILGEVNL
                     SDPAPSRFIELWLYDHPPLGERVAFARGYDPWAKGEPTRYVREKMP"
     gene            complement(1468..>1820)
                     /locus_tag="VF958_01755"
     CDS             complement(1468..>1820)
                     /locus_tag="VF958_01755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9147863.1"
                     /translation="ARAPIHGRAMKCLPVAIALLVVLGGCATRATSALDALSPAALAA
                     MTDLAKTTAAPVSEITIVSETDTTWPDACLGCPGTGEMCAQVMARGSRVVLLARGVTY
                     EYHADRSGTVRRCR"
BASE COUNT          341 a          601 c          642 g          236 t
ORIGIN      
        1 gcgcggaagt tccgatcgag gatccgatag ccggcttcga cgagcgcctt ctcggcgacg
       61 cgctcccagt cccagcccgt gccgcgagag ccgccgggac gccggggcag cgccgacatc
      121 cattcgacga ttcgctcgaa gatcgcccgc ggcgtcacgg catcttctcc ctcacgtagc
      181 gcgtcggctc gcctttcgcc caggggtcgt accccctcgc gaacgcgacg cgttcgccga
      241 gcggcggatg gtcgtacagc cacagctcga tgaagcggct cggggcgggg tccgagaggt
      301 tgacctctcc caggatctgg aaagcctctg cggcggtgcg ctgcgagtcg gggacgaggc
      361 cgtgaatgac ctcgagcccg tagatgtcgg ccgcgtgttc ctgcatccgg ctgtacgacg
      421 cgaccaccgg cgaggcgatc tcgccgaaga cggtgacgac gagcatcagg gccggaagcg
      481 acgcccagtc ggtgaccccc cggattcccc ggtactcccg cccctccggc aggacccgcc
      541 ggagcacccc gtgcgcgaga aagaggagga cgagggagag cgccgcgacg aaagcgatgg
      601 tcttcggaat gtgcgcgagc acgtaatggc ccatctcgtg cccgaaaacg aagagcgtct
      661 gcgggatcgt cattcggtcc aaagtcgtgt cccacacgac gacgcgtttc gatccgccga
      721 accccgtcac gtaggcgttc agggacttca gcttcctgct cgcgtccatc aggaacatcc
      781 gctcgcgcgg gatcgagagg ccaccgcgtt ccgtcacact ttcgatcctc tcgacgagct
      841 gcggcgcctt tccggcaagc ggctggaagt cgaagaaaag cggatcgacg acgaccgggg
      901 cgatgaaaac gacgaaaacg atgatcggaa gagtcgcgag ccagaaccag agccaccagc
      961 ggcgcgggct gcgtcggagg atcccgtaga ggacccagac gacgggaacg aagagggcga
     1021 gcgcgatcag gacgcccttc agccagtccc agagccagga gtcccacgcc tggatcgact
     1081 ggccgtagaa aacggagaga cggtgccccc acacggcgag aggcaggtcg aggacctgct
     1141 ccgtcagcag gaggagtgga acgaacacga gcgcctggac gaagcgccgt cgcgagagcc
     1201 gttcggcgag gtcgcggtag cgcggtgcga cccgcagcgc gatgagccct cccagaagaa
     1261 ggacttccca cgcgaagccg aggaagtgca ggcggtattt cgcccgcgag aaggcgaccg
     1321 cctggcggta cttgtccggc gggagcgtgt acgcagcggg agcgggcccg acggtcgggt
     1381 tggcggtcgg ctgcggcgca ggcggcggtg cggcgagaag cgcggcggga atggtcagcg
     1441 caccgaggac gacgaggaag cgtcgcctca ccggcaacgg cgcacggtgc cggaccgatc
     1501 cgcgtggtat tcgtaggtca cgccgcgcgc gagaagaacg acacgcgagc cacgcgccat
     1561 cacctgcgcg cacatctccc ctgtccccgg gcaacccagg caggcgtcgg gccaggtcgt
     1621 gtcggtctcg gacacgatgg tgatctcgga caccggcgcc gccgttgtct tcgcgaggtc
     1681 cgtcatcgcg gcgagagcgg cgggcgaaag cgcgtccagg gcgctcgtcg cccgcgtcgc
     1741 acatccgccg agcacgacga ggagggcaat cgcgaccgga agacacttca tcgcgcggcc
     1801 atggatcggc gcgcgagctc
//
LOCUS       DASWAV010000079         1892 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U2665
            k141_524061, whole genome shotgun sequence.
ACCESSION   DASWAV010000079 DASWAV010000000
VERSION     DASWAV010000079.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960316
            Sequence Read Archive: SRR10260007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 1892)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1892)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:47:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,929
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1892
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_524061"
                     /isolate="SMAG_U2665"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.36444 N 132.31889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(112..1461)
                     /locus_tag="VF958_01760"
     CDS             complement(112..1461)
                     /locus_tag="VF958_01760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020708202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9147864.1"
                     /translation="MKRWRPVLPLLPVLLVGFALLTGPRNVEGNPAVEAKRPAAGFDS
                     RINEHSNQMIEEGRKTFRFETFGSEAFWGDVLQLHKAIAGEKNGGVGPGVSPKTALSV
                     GLKVDADALPDGLKKQLAAGKVDLDDPATTLALLKLNAVVGLTGHFDPKGGIHSIGIQ
                     CALCHSTVDDAFAPGIGKRLDGWAANDLNVGAIVSLAPNLQPFGDLLGVDIPTVRKVL
                     ASWGPGRYDAVLDKDGKAFRPDGKQAGTVIPPAFGLAGVNLHTWTGFGSVTYWNAYVA
                     VTQMHGSGTFFDARFNDKDQYPVAAKSGSGNSRGKPDLVTSKLAALHFYQLAIPAPKP
                     PAGSFNTVAAARGKEVFNAKGQCATCHVPPLFTEPGNNLHAPSEIGVDAFQADRSPTH
                     MYRTSPLAGLWSHPKAGYYHDGRFKNLRDVVDHYDTFRGLKLTEQEKLDLVEYLKSL"
     gene            complement(1608..>1892)
                     /locus_tag="VF958_01765"
     CDS             complement(1608..>1892)
                     /locus_tag="VF958_01765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9147865.1"
                     /translation="GDTAAVQTESARWNANADEIADFLNGANPQNWPRQTLHSEMRHH
                     LDLTLQEAQARLRGDWAADIAAYDAVHQHILGMSDVLSAGIVKQFPRRFK"
BASE COUNT          303 a          589 c          652 g          348 t
ORIGIN      
        1 tctcaggaac cgtcagggga cttcggcgct ggggtgggcg gatcataccc ctcggcgtcc
       61 tgcgaaagaa cgggcgtcct cggagggcgc tcgccccccg aggttccgct cctagagcga
      121 tttcaggtat tcgacgaggt cgagcttctc ctgctccgtc agcttcaggc cgcgaaaggt
      181 gtcgtagtgg tcgacgacgt cgcgaagatt cttgaaacgg ccatcgtggt agtagcccgc
      241 cttcgggtga ctccagagcc ccgcgagcgg cgaggtgcgg tacatgtgcg ttggcgatcg
      301 gtcggcctgg aaggcgtcga cgccaatctc gctcggagcg tgcaggttgt tccccggctc
      361 ggtgaagagc ggcggcacgt gacacgtcgc gcactgccct ttggcattga agacctcctt
      421 cccgcgcgcc gctgcaacgg tgttgaagga accggccggc ggcttcggag ccgggatggc
      481 gagttgatag aagtgaagag cggcgagttt tgaagtgacg agatccggct tgccccgcga
      541 gtttccggac ccgctcttcg cggcgaccgg gtactgatcc ttgtcgttga agcgagcatc
      601 gaagaaggtt cccgagccgt gcatctgggt cacggccaca taggcgttcc agtacgtgac
      661 cgatccgaag ccggtccagg tatgcaggtt cacgccggcg agaccgaagg ccggcggaat
      721 gacggtgccc gcctgtttcc cgtccggacg gaaggccttg ccgtccttgt cgagaacggc
      781 gtcgtagcga ccgggccccc agctcgcgag aaccttccgg acggtcggga tatccacgcc
      841 gaggagatcg ccgaaaggct ggaggttcgg tgcgagcgag acgatcgcgc cgacgttcag
      901 gtcgttggcg gcccagccgt ccagtcgctt tccgataccg ggcgcgaagg cgtcgtcgac
      961 ggtcgagtgg cagagcgcgc attggatccc gatggagtgg atgccccctt tcgggtcgaa
     1021 atggccggtg agcccgacga cggcgttcag cttgagcaga gcgagggtcg ttgcggggtc
     1081 gtcgaggtcg accttgccgg ccgcgagctg tttcttgaga ccgtcgggca gcgcatccgc
     1141 gtccaccttc aggccgaccg acagcgccgt cttcgggctc actcccggtc caacgccgcc
     1201 gttcttctct ccggcgatgg ccttgtggag ttgaagcacg tctccccaga aagcctcgct
     1261 gccgaaggtc tcgaagcgga aggtctttcg cccttcctcg atcatctgat tcgagtgttc
     1321 gttgatccgg ctgtcgaaac ccgcagccgg cctcttcgcc tcgacggccg gattgccctc
     1381 gacgtttcga ggcccggtca gaagagcaaa gccgacgagc agaaccggga gtagcgggag
     1441 aaccgggcgc cagcgtttca tgggacctcc gatctcgaaa tgcagtctca cgacgggcgc
     1501 cgaccggcga ggtcggcggt gcggatggga ccgggctcgg atatcagtgc gcgcgcggcg
     1561 ttgacctgaa ccaggtgttc cacggcagac gccggccggc cgtgcatcta tttgaacctc
     1621 cgcgggaatt gcttgacgat gcccgcggac agcacgtccg acatcccgag gatgtgctga
     1681 tggaccgcgt cgtacgccgc gatgtccgcc gcccagtcgc cgcgaaggcg cgcctgcgct
     1741 tcctggagcg tcagatcgag gtgatggcgc atttcggagt ggagggtctg gcgcggccag
     1801 ttctgagggt tcgccccgtt caggaagtcg gcgatctcgt cggcgttcgc gttccagcgc
     1861 gccgactcgg tctgaaccgc cgccgtgtct cc
//
LOCUS       DASWAV010000080         2406 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U2665
            k141_3925, whole genome shotgun sequence.
ACCESSION   DASWAV010000080 DASWAV010000000
VERSION     DASWAV010000080.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960316
            Sequence Read Archive: SRR10260007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2406)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2406)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:47:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,929
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2406
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3925"
                     /isolate="SMAG_U2665"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.36444 N 132.31889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1380
                     /gene="glgB"
                     /locus_tag="VF958_01770"
     CDS             <1..1380
                     /gene="glgB"
                     /locus_tag="VF958_01770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012871574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003844 - 1,4-alpha-glucan branching
                     enzyme activity [Evidence IEA];
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0043169 - cation binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-alpha-glucan branching protein GlgB"
                     /protein_id="HEX9147866.1"
                     /translation="GSWGYQMTGFFAPTSRYGGPQDLMFLIDQLHEKGIGIIFDWVPS
                     HFPSDEHGLVFFDGSYVYEHADPRQAVQPDWGSLVFNYGRNEVRSFLLSSALFWLDVY
                     HGDALRVDAVASMLYLDYSRKEGEWVANQFGGRENLEAIAFLRRFNEEVYRTVPGAET
                     IAEESTAWPMVTRPTWVGGLGFGMKWDMGWMHDTLEYMKLDPVHRKYHHDKLTFRGLY
                     AFTENYVLPLSHDEVVHLKGSLLAKMPGDDWQKLANLRLLFAWMWALPGKKLLFMGGE
                     IGQWREWNHDRELDWELLGQPEHSGLQRWVRDLNGLHARERALHELDFEPAGFSWIDC
                     TDSEHSVVAVLRRGRSEADVVAAAFNFTPVPRYGYRIGVPIEGFWSEIANSDASEYGG
                     SGVGNLGGSQAKELPAHGHPYSIELTMPPLGAVFLKPEKCPADDPSPQPSPRERGEGV
                     RFGETDGEP"
     gene            1377..>2406
                     /locus_tag="VF958_01775"
     CDS             1377..>2406
                     /locus_tag="VF958_01775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013823875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase"
                     /protein_id="HEX9147867.1"
                     /translation="MNRFVTVHGHFYQPPRENPWTGEVDQEVTARPFHDWNERITAEC
                     YAPNAPNYSRMSFNFGPTLLAWLEANAVEAYRAILEADRASRERFSGHGSAIAQAYNH
                     AILPLANRRDKITQVAWGIRDFEHRFGRRPEGMWLPETAVDDATLEALAAEGIAFTIL
                     APSQAAAVREPASRWMEIQGGGIDPREAYRVPLPSGRTISVFFYDGGLSHGIAFGEIA
                     RGGEDFARALLSRFDSRSGPQLVHVATDGETYGHHFRGGERTLGEALERVEDSGHARL
                     TNYGEFLERFPPRLEAQIVPNSSWSCAHGVGRWSDECGCSAGAAVSHPWRRPLREALD
                     WLRDTLAPL"
BASE COUNT          405 a          793 c          824 g          384 t
ORIGIN      
        1 ggatcgtggg gataccagat gaccgggttc ttcgcgccca cgagccgcta cggcgggccg
       61 caggacctga tgttcctgat cgaccagctg cacgagaagg ggatcggcat catcttcgac
      121 tgggtgccct cgcacttccc gtccgacgag cacgggctcg tcttcttcga cggcagttac
      181 gtctacgagc acgccgaccc gcggcaagcg gtgcagccgg actggggcag ccttgtcttc
      241 aactacggcc ggaacgaggt gcgcagcttt ctgctctcga gcgccctctt ctggctcgac
      301 gtgtaccacg gcgacgcgct gcgcgtggac gccgtcgcgt cgatgctcta cctcgactac
      361 tcgagaaaag agggggagtg ggtcgcgaac cagttcggcg gtcgcgagaa cctcgaggcg
      421 atcgccttcc tgcgtcgttt caacgaagag gtctaccgga ccgtccccgg cgcagaaacg
      481 atcgccgaag aatcgacggc gtggccgatg gtcacgcgac cgacgtgggt gggggggctc
      541 ggtttcggca tgaagtggga catgggctgg atgcacgaca cgctcgaata catgaagctc
      601 gatccggtgc accggaagta ccaccacgac aagctcacgt tccggggact ctacgcgttc
      661 acggaaaact acgtcctgcc gctctcgcac gacgaggtcg tgcacctgaa ggggtcgctc
      721 ctcgcgaaga tgccgggcga cgactggcag aagctcgcca acctgcggct gctcttcgcg
      781 tggatgtggg cgctccccgg gaagaaactc ctcttcatgg gcggtgagat cgggcagtgg
      841 cgcgagtgga atcacgaccg ggagctcgat tgggaattgc tcgggcagcc ggagcactcc
      901 ggcctgcaga gatgggtccg ggacttgaat ggtctccacg cccgcgagcg cgccctgcac
      961 gaactcgact tcgaacccgc ggggttttcc tggatcgact gcacggactc cgaacacagc
     1021 gtcgtcgccg ttctgcggcg cgggcgctcg gaagcggacg tggtggcggc cgccttcaac
     1081 ttcacgccgg taccgcgcta cggctaccgc atcggagtgc cgatcgaagg cttctggtct
     1141 gagatcgcca acagcgacgc gtccgaatac ggcgggagcg gggtcgggaa cctcggcggc
     1201 agccaggcga aggagcttcc cgcgcatgga catccctact cgatcgagct cacgatgcct
     1261 ccgctggggg cggtgttcct gaagccggaa aagtgccccg ccgatgaccc ctcaccccaa
     1321 ccctctcccc gcgagcgggg agagggagtg cgcttcggtg agacggacgg tgagccctga
     1381 accgcttcgt aacggtccac ggccacttct accagccgcc tcgggagaac ccgtggacgg
     1441 gggaggtgga ccaggaggtc acggcgaggc cgtttcacga ctggaacgag cgcatcaccg
     1501 cggaatgcta cgcgccgaac gccccgaact actcgcgcat gagcttcaac ttcggaccga
     1561 cattgctcgc gtggctcgag gcgaacgcgg tcgaggcgta tcgcgcgatc ctcgaggcgg
     1621 accgcgcgag ccgggagcgc ttttcgggac acgggtcagc gatcgcgcag gcttacaacc
     1681 acgcgatcct gccgctcgcc aaccgccgcg acaagatcac gcaggtcgcc tggggaatcc
     1741 gggacttcga gcaccgtttc ggccgcaggc cggagggcat gtggcttccc gagacggcgg
     1801 tcgacgacgc gaccctcgag gcgctcgcgg cggaggggat cgccttcacg atcctcgcgc
     1861 cgtctcaggc ggcggcggtg cgcgagccgg cctcacgctg gatggagatc cagggaggcg
     1921 gaatcgatcc gcgcgaggcg tatcgcgtgc ctctcccttc gggccgcacg atctcggtct
     1981 ttttctacga cggcggactc tcccacggca tcgcgttcgg cgagatcgcg cgcggcgggg
     2041 aggacttcgc gcgggcgctc ctttcgaggt tcgacagccg gagtggaccg cagctcgtgc
     2101 acgtcgccac cgacggagag acctacggcc accactttcg cggcggagaa cgcacgctcg
     2161 gagaagcgct cgaacgcgtc gaggattccg gccatgcgcg gctgacgaac tacggcgagt
     2221 tcctcgagcg gttccccccg cgcctcgagg cgcagatcgt cccgaactcc tcgtggagct
     2281 gcgcgcacgg agtcggccgc tggagcgacg agtgtggttg ctcggccggc gctgcggtca
     2341 gccatccgtg gcggcggcca ctgcgcgagg ccctcgactg gctgcgtgac acgctcgctc
     2401 ccctct
//
LOCUS       DASWAV010000081         1915 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U2665
            k141_244397, whole genome shotgun sequence.
ACCESSION   DASWAV010000081 DASWAV010000000
VERSION     DASWAV010000081.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960316
            Sequence Read Archive: SRR10260007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 1915)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1915)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:47:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,929
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1915
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_244397"
                     /isolate="SMAG_U2665"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.36444 N 132.31889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..555
                     /locus_tag="VF958_01780"
     CDS             <1..555
                     /locus_tag="VF958_01780"
                     /inference="COORDINATES: protein motif:HMM:NF024579.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9147868.1"
                     /translation="DERQFFNLAEELEKELSEESTPPAAPALAETQDEASLQDIFREF
                     RKGVEQQLSAEDYETHHNLGIAYKEMGLLDEAIGEFQLASKAPALAIECCSLLGLCFL
                     EKGMPQLAIKWYGKGLATPGIRESETAGLLYDLARVYQDSGDMNQAYKTFLEVYGINT
                     NYRDVVERVRHLEEATKNSRESVH"
     gene            645..1589
                     /locus_tag="VF958_01785"
     CDS             645..1589
                     /locus_tag="VF958_01785"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX9147869.1"
                     /translation="MPVAGEFVPAVDRVSFDLAAGEAVALVGESGCGKTLTGRALLGL
                     APEGAHVSGSIRLRGAELTGASEEEWRRVRGRQVALVFQEPGAAFDPVATVGSQIVEA
                     VRAHRRIDLREAGALAQERLREVGFPDPGRGMSEYAHRLSGGLKQRAFIAMALASDPA
                     VLVADEPTTALDATVAAQVLELLDRLRRERGLALLLISHDLGVVAAHAERVLVMYAGR
                     VAEEIAAADLFRAPRHPYTAGLVASRPRLAAPDRERKFPAIPGAVPDLASRPRGLCAF
                     VPRCPARFEPCDVRDPELYATDVGLARCFLYERREVNP"
     gene            1586..>1915
                     /locus_tag="VF958_01790"
     CDS             1586..>1915
                     /locus_tag="VF958_01790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020755976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX9147870.1"
                     /translation="MNATAPLLSARELVKRYPLRSGPFRRDRTFAAVDGISFDVGRGE
                     TLGLVGESGSGKSTTGRLLLRLEEPTEGRILFEGENWLAVEGAELRRRRRDIQIVFQD
                     PQTSLNPR"
BASE COUNT          297 a          654 c          666 g          298 t
ORIGIN      
        1 gacgagcggc agttcttcaa cctcgccgag gagctcgaga aggagctctc ggaggagtcc
       61 acgccgccgg cggcgccggc gcttgccgaa acacaggacg aagcttctct ccaggacatc
      121 ttccgggagt tccgcaaggg cgtcgagcag cagctctcgg cggaggacta cgagactcac
      181 cacaatctcg ggatcgccta caaggagatg ggccttctgg acgaagcgat cggcgagttc
      241 cagctcgcga gcaaagcgcc ggcgctcgcg atcgagtgct gcagccttct cggactctgc
      301 tttctcgaga aggggatgcc gcagctcgcc atcaagtggt acggcaaggg tctcgcgacg
      361 cccggcatcc gcgagtccga gaccgccggg ctcctctacg atctcgcccg cgtctaccag
      421 gacagcggag acatgaacca ggcctacaag accttcctcg aggtctacgg gatcaacacg
      481 aattaccggg acgtcgtcga gcgggttcgg cacctcgaag aggcgacgaa gaactcgcgc
      541 gagtccgttc actgacggga gccaaagtct caagtcggca gcggagcgct tcccccggag
      601 ccataccctc ggctctgctc gaagtccgag acctcaccgt cgaggtgccc gtcgcaggag
      661 agttcgtccc cgcggtcgat cgcgtgagct tcgacctcgc ggcgggagag gcggtggctc
      721 tcgtcggcga gagcggctgc ggaaagacgc tcaccggtcg ggcgctcctc ggcctggcgc
      781 cggagggggc gcacgtgagc ggttcgatcc ggttgcgcgg cgccgagctc accggggcgt
      841 ccgaagaaga gtggcggcgc gtccggggcc gccaggtggc gctggtgttc caggagccgg
      901 gcgccgcgtt cgatccggtg gcgacggtcg gctcgcagat cgtggaagcc gtccgcgcgc
      961 accgtcgcat cgacctccga gaggcgggcg cgctcgcgca ggagcgtctc cgcgaggtcg
     1021 ggtttcccga tcccgggcgc ggaatgagcg agtacgcgca ccgtctctcc ggcggcctca
     1081 agcagcgcgc gttcatcgcg atggcgctcg cgtctgatcc cgcggtcctc gtggccgacg
     1141 agcccacgac ggcgctcgac gcgacggtgg cggcgcaggt actggagctg ctcgaccggc
     1201 tccggcggga gcgcgggctc gccctgcttc tgatcagcca cgacctcggg gtcgtcgccg
     1261 cccatgccga acgcgtcctg gtgatgtacg ccggtcgcgt ggccgaggag attgccgccg
     1321 ccgacctctt ccgcgcgccg cgccatccct acaccgccgg gctcgtcgct tcgcgtcccc
     1381 ggctcgccgc gccggatcgc gagcgaaagt tcccggcaat cccgggcgcc gtcccggacc
     1441 tcgcctcgcg tccccgggga ctctgcgcgt tcgttccccg ctgtccagcg cgcttcgagc
     1501 cgtgcgacgt acgggacccg gagctctatg ccaccgatgt cgggcttgcg cgatgcttcc
     1561 tgtacgaacg acgagaagtg aatccgtgaa cgccacggcg ccgcttcttt ccgcccggga
     1621 gctcgtgaag cggtatccac tgcgctcggg gccttttcgc cgcgaccgaa ccttcgctgc
     1681 ggtcgacggc atttcgttcg acgtcggacg cggagagacc ctcgggctcg tgggggagtc
     1741 gggaagcgga aagagcacga ccggcaggct cctcctgcgc ctcgaggaac cgaccgaagg
     1801 acgcattctg ttcgaggggg agaactggct cgcagtcgag ggcgcggagc tgcgccggag
     1861 gcgccgggac atccagatcg tttttcagga tccgcagacg tctcttaacc cgcgg
//
LOCUS       DASWAV010000082         4386 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U2665
            k141_485089, whole genome shotgun sequence.
ACCESSION   DASWAV010000082 DASWAV010000000
VERSION     DASWAV010000082.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960316
            Sequence Read Archive: SRR10260007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 4386)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4386)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:47:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,929
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4386
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_485089"
                     /isolate="SMAG_U2665"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.36444 N 132.31889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..382)
                     /locus_tag="VF958_01795"
     CDS             complement(<1..382)
                     /locus_tag="VF958_01795"
                     /inference="COORDINATES: protein motif:HMM:NF013970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HEX9147871.1"
                     /translation="MISYLHDTSCLVAAVCAWHRFHDATRREIERRNAAGEKLILSAH
                     SLAEAFSVLTRLPEPHRLRPSDALAVIEANWGETGLVALTGPDYRATLRRCRDLGIGG
                     GAVYDALIAACARKARVATLVTWDL"
     gene            complement(379..654)
                     /locus_tag="VF958_01800"
     CDS             complement(379..654)
                     /locus_tag="VF958_01800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003413429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="HEX9147872.1"
                     /translation="MKTTIDRAGRLVVPKKIREAAGIVPGSELTIRVADGRIEIEPAP
                     LDVRLVKRGSLTVAVPRKHVTPLTPEVVGQTLDRLRRRRGGGAGENE"
     gene            complement(701..3355)
                     /locus_tag="VF958_01805"
     CDS             complement(701..3355)
                     /locus_tag="VF958_01805"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HEX9147873.1"
                     /translation="MSLAAGSRLGPYEIVTPIGAGGMGEVYRARDPRLGRDVAIKVLP
                     ASFSKDPERLRRFEQEARAAGVLNHPNIMAVYDIGTVEGSPYVVSELLEGETLRSRLG
                     GGPLAPRRATEYAVQIAHGLSAAHEKGIVHRDLKPENLFVTKDGRVKILDFGLAKLTQ
                     PDGGGPQTNFPTAAASAETEPGVVLGTLGYMSPEQVRGRPADARSDIFSFGAILYEML
                     SGKRAFHRDTAADTMSAILREDPPDLSSTNRLVSPALDRIIRHCLEKDPEARFHSAHD
                     LAFQLQLPATDVISAIAAAPSAKPRLPPAVLATGAIALVAGAALAALFLRRPPEAHSV
                     RPVRFAVPIPPGTTYSPSDISRGVSISPDGARLVIEALSNGRRRLFLRPLDSEKFTEL
                     EGSLGASAHFWSPDSRFIAFFADGKLKKIPATGGPAEELCTAPYDWVGTWGRGGTILF
                     SQIPPGVPGIYSVSDRGGEPVRVLGVDPSDPVALLWPQFLPDGRRFLFQALAPNMTSR
                     ELRVGSLGSREVRSITQLASRVEYAQPGFLVYVRENALFAQPFDATKGVLSGEPSLLA
                     ENVHHFFGPGHAAFSVSQAGVIAYQAAETPVRLAWLDRQGKEIGVLGQPAVVDFVRIS
                     PNGNRIAVDVEDSRFGTSDLWVFEAASGASTRLTSGQIDENAPVWTPDGSRLFFRTDA
                     HGPPDIAQIVVGSPGSERPLLTQPGVQQPEDVSQDGRRLVFLENAATTADIWLMSLDG
                     EPRPTPWLRSAFNERNPRFSPDGRWIAYDSDESGTPEVYVALTDGAGEKKRLSPGGGR
                     QPRWRRDGKELDYIAPDGTIMAIPVVLGAALQAGAPAPLFHVETGVRNFDLTPDGNRF
                     LVSTPVEKSPESPIRVILNWDAALKREK"
     gene            complement(3417..4091)
                     /locus_tag="VF958_01810"
     CDS             complement(3417..4091)
                     /locus_tag="VF958_01810"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019500452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DJ-1/PfpI family protein"
                     /protein_id="HEX9147874.1"
                     /translation="MPEKPQIPGFRLGIYVFKDAEIVDFAAPHGVFSVARRFDPELDA
                     FLIADALRPVQTQAGFTVLPNYSFQDSPAMDAFLLPGGFGTRQELNNRRLHDYIRALP
                     DSTLLTSVCTGSWIYGKMGLLDGLPATNRKEPDRLESSPAGMVPIDRLARIAPAARIS
                     RARVVDAGRIITAGGIASGMEMGFHLLRRAGYSEEFVAEVARVMEYQKAYELYRDDVE
                     TSGRGR"
     gene            complement(4178..>4386)
                     /locus_tag="VF958_01815"
     CDS             complement(4178..>4386)
                     /locus_tag="VF958_01815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020714590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HEX9147875.1"
                     /translation="TSFNDSQVMYWEPAKYVARLRATKTDSNPLIFKVNMAGGHGGSS
                     GRYDRLREAAFDYAFLLNQLHLGG"
BASE COUNT          733 a         1400 c         1541 g          712 t
ORIGIN      
        1 cgagatccca ggtcaccagc gtcgcgaccc gcgccttgcg cgcgcaagcc gcgatgaggg
       61 cgtcgtagac cgcgccgccg ccgatcccga ggtcgcggca gcggcgcaac gtcgcgcggt
      121 agtcggggcc ggtcagcgcg acgagccccg tctctcccca gttcgcctcg atcaccgcca
      181 gagcgtcact cggacgcagc cggtgaggtt ctggaagccg tgtcaggaca gagaaggcct
      241 ccgcgagcga gtgcgccgac aggatgagct tctcgccagc tgcgttgcgg cgctcgatct
      301 cccgccgagt ggcatcgtga aaccggtgcc aggcgcagac ggctgcgacg aggcaactcg
      361 tgtcgtggag gtaggagatc actcgttctc gccggcgccg ccaccgcgcc gccgacgaag
      421 ccggtcgagc gtctgtccga cgacctccgg ggtcagcggg gtgacgtgct ttcgcggaac
      481 cgccacggtc agcgatcctc gcttgacgag ccgcacgtcg agcggggccg gctcgatctc
      541 gatacggccg tcggcgacgc ggatggtcag ctccgagccg ggcacaatcc cggcggcctc
      601 ccggatcttc ttgggcacaa cgagccgtcc ggcgcggtcg atggttgttt tcatggtaaa
      661 taatctacca ctatctatgc caatcggggt cggggcggca tcacttctcc cgcttcagcg
      721 ccgcgtccca gttgaggatg acgcgaatcg gcgattcggg ggatttctcg accggcgtgc
      781 tcacgaggaa tcgatttcca tcgggagtca ggtcgaagtt gcggaccccg gtctcgacgt
      841 ggaagagggg cgccggcgct cccgcctgga gcgccgctcc gaggacgacg ggaatggcca
      901 tgatggtccc gtcgggtgcg atgtagtcga gctcctttcc atcccggcgc cagcggggtt
      961 gtcgcccgcc gcccggagaa aggcgcttct tctctccggc gccgtcggtg agtgcgacgt
     1021 agacctccgg agtcccggat tcgtccgagt cgtaagcgat ccatcgtccg tccggtgaaa
     1081 agcgcggatt tcgctcgttg aacgcggagc gaagccacgg cgtcggccgc ggttcaccgt
     1141 cgagggacat cagccagatg tccgcggtcg tcgcagcgtt ctccaagaat acgagacggc
     1201 ggccgtcctg cgaaacgtcc tcgggttgct ggacgcccgg ctgagtcagc agcggccgct
     1261 cgcttccggg cgacccgacg acgatttggg cgatgtccgg cggtccgtgc gcgtcggtcc
     1321 ggaagaaaag gcgtgacccg tcgggcgtcc acacgggagc gttttcatcg atctgacccg
     1381 aggtcaatcg ggtggaagcc ccgctcgcag cctcgaagac ccagagatcg gatgtcccga
     1441 accggctatc ctcgacgtca acggcgatgc gattcccgtt gggcgagatc cgcacgaagt
     1501 cgaccaccgc gggctggccg agaacgccga tctccttacc ctgccggtcg agccacgcga
     1561 gccgcacggg agtctccgcg gcctggtatg cgatgacacc ggcctgggag accgagaagg
     1621 cggcgtgccc cggtccgaag aagtggtgga cgttctcggc caggagggat ggctccccgg
     1681 agagaacgcc tttcgtggcg tcgaagggct gcgcgaacag ggcgttctcg cggacgtaga
     1741 cgaggaaacc gggctgcgca tattcgacac gggaggcgag ctgggtgatc gagcggacct
     1801 ctcgcgagcc gagcgaccca acccgcagct ctctcgacgt catgttcggt gcgagtgctt
     1861 gaaagagaaa acgccggccg tctgggagga actgaggcca cagaagcgcg accgggtcag
     1921 agggatcgac gcccaggacg cggacaggct ctccgccccg gtccgagacg ctatagattc
     1981 cgggcactcc cggcgggatc tgcgagaaga ggatcgtccc gccccgcccc cacgtcccga
     2041 cccagtcgta cggagccgtg cagagctctt ccgcagggcc tccggtcgcc gggatcttct
     2101 tcagcttgcc gtccgcgaag aacgcgatga agcggctgtc tggcgaccag aagtgcgcgc
     2161 ttgctcccag ggagccctcg agctcggtga acttctcgga gtcgagcgga cgcaggaata
     2221 gtcggcggcg accgttcgac aaggcctcga tgacgaggcg agccccgtcc ggtgagatcg
     2281 agacgccccg cgagatatcg gacggagagt aggtcgtccc gggggggatc ggcaccgcga
     2341 agcgcaccgg ccgcaccgaa tgggcttccg gaggccggcg gaggaagagt gcggcgaggg
     2401 ccgccccggc gacgagcgcg atcgcgccgg tggcgagaac cgccggcggc agacgcggct
     2461 tcgcggaagg cgccgccgcg attgcggaga tcacgtcagt cgccggcaac tgcagctgga
     2521 acgcgaggtc gtgcgcggag tggaacctgg cctcggggtc cttctcgagg cagtggcgga
     2581 tgatccggtc gagggcgggc gaaaccagcc ggttcgtcga ggagaggtcc ggtggatcct
     2641 cgcgcaggat cgccgacatc gtgtcggccg cggtgtcgcg gtggaaggcc ctctttccgg
     2701 agagcatctc gtagaggatc gcgccgaacg aaaagatgtc gctgcgcgcg tcggccggcc
     2761 tgccccgcac ctgctcgggt gacatgtatc cgagggtgcc cagaacgacg cccggttccg
     2821 tttccgcgct cgcggccgcc gtgggaaagt tcgtctgggg tccgcctccg tctggctgcg
     2881 tcagcttcgc cagcccgaag tcgaggatct tgacgcgccc gtctttcgtg acgaaaaggt
     2941 tctcgggctt caagtcccgg tggacgatcc ccttttcgtg cgcggccgaa aggccgtgcg
     3001 caatctggac ggcgtactcg gtcgcgcggc gcggggcgag tggtcctccc ccgagtcggc
     3061 tccggagcgt ctcgccctcc aggagttccg agacgacgta gggcgagcct tcgacggtgc
     3121 cgatgtcgta caccgccatg atgttggggt gattcaggac gccggcggcg cgggcctcct
     3181 gctcgaagcg gcgcagcctc tcggggtctt tcgaaaagga ggcgggaaga actttgatcg
     3241 caacgtcccg cccgaggcgc ggatcgcgcg ccctatagac ctcgcccata ccgccggcgc
     3301 cgatcggcgt gacgatctcg tagggaccga gacgggaacc cgcggcgagg ctcatcaggc
     3361 gatcagccgg aagaacgggc gaccgtgacc tcgccgggat ggttcatcgg ccggtcttat
     3421 cgcccgcgtc cggatgtctc gacgtcgtcg cggtagagct cgtacgcctt ctgatactcc
     3481 atgactcgcg cgacttccgc gacgaactcc tcgctgtatc cggcgcgccg caggaggtga
     3541 aaacccatct ccatcccgga agcgatgccg ccggcggtaa tgatgcgccc cgcgtcgacg
     3601 actcgagccc gactgatgcg ggccgccggc gcgatccggg cgagccggtc gatcgggacc
     3661 attccggcgg gcgaagattc gaggcgatcc ggctccttgc ggttggtcgc cggaaggccg
     3721 tcgagaagcc ccatctttcc gtagatccag gatccggtgc agacgctcgt gaggagcgtc
     3781 gagtcgggaa gcgcgcggat gtagtcgtgg agccgtcggt tgttcagctc ctggcgggtg
     3841 ccgaagcctc ccgggagcag aaaagcatcc atcgcggggg agtcctggaa cgagtagttc
     3901 gggagaaccg tgaagcccgc ctgcgtctgc acgggacgaa gggcgtcggc gatcaggaac
     3961 gcgtcgagct ccggatcgaa ccgccgggcg acggaaaaga ccccgtgggg tgcggcgaag
     4021 tcgacgattt ccgcgtcctt gaaaacgtag atgccgagtc gaaatccggg tatctgcggc
     4081 ttctccggca tgtcgtcctc ccgctcgtgg gcttcgggca aaagtctatg ccggtccgtg
     4141 ccgcaaattg gatccgactc gtcgacggtg aaggttgtca gccgccgagg tggagctggt
     4201 tcagcaggaa tgcgtagtcg aacgcggcct cacggagcct gtcgtatcgc ccggaagatc
     4261 caccatggcc gccggccatg ttgaccttga agatcagagg attcgagtcg gtcttcgtcg
     4321 cgcggagtct cgcgacgtac tttgcaggct cccagtacat gacctggctg tcgttgaaag
     4381 aagtct
//
LOCUS       DASWAV010000083         4511 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U2665
            k141_684370, whole genome shotgun sequence.
ACCESSION   DASWAV010000083 DASWAV010000000
VERSION     DASWAV010000083.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960316
            Sequence Read Archive: SRR10260007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 4511)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4511)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:47:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,929
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4511
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_684370"
                     /isolate="SMAG_U2665"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.36444 N 132.31889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(179..385)
                     /gene="thiS"
                     /locus_tag="VF958_01820"
     CDS             complement(179..385)
                     /gene="thiS"
                     /locus_tag="VF958_01820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012522401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur carrier protein ThiS"
                     /protein_id="HEX9147876.1"
                     /translation="MEVRVRVNGDERTFPDGTSIAALLVALGVSTPRVAVERNREIVP
                     KSAYSSTHLLEGDALEVVEFVGGG"
     gene            complement(415..1161)
                     /locus_tag="VF958_01825"
     CDS             complement(415..1161)
                     /locus_tag="VF958_01825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9147877.1"
                     /translation="MITGYNTDVKHKDRVFHIQTEDRGDQNPYVESFVYVGGEILGGK
                     RTAYPESLRNGADERAVRELMEHQHRDLIASVREGSFEGADGSVRVPDDMVLGGITSP
                     TGAGSEVRERPAQRVSPRRPSDSARISGDRSLDQVILDYLASEGNPEQIDLTFSPAPE
                     FVSGKRINVRLRAELALSRRPVAGASVQIRVLSSAKPVPVFAGMTGPDGSCEVTFLMP
                     TIPTGTAAAVVRLTSAAGSTEIQYPVRRGV"
     gene            complement(1197..2573)
                     /locus_tag="VF958_01830"
     CDS             complement(1197..2573)
                     /locus_tag="VF958_01830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011645837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophanase"
                     /protein_id="HEX9147878.1"
                     /translation="MRELSPEPWRVKVVERIRSISPQEREEALGRAGYNVFGLDSEDI
                     YIDLLTDSGTSAMSDEQWAGLMIGDESYAGGRNYRHFERTVREIFGLPHVIPTHQGRV
                     AENLLFSTVLKPGDVVPNNTHFDTTRANVEVNGGIALDFVIPEGKSPRLRHPFKGNMD
                     LARLERCLEENRGRVPLVMLTLTNNSGGGQPVSLENIRATHDLCARHGVPLVIDACRF
                     AENAWFIKEREAGQHGRSVTEIVREIFSLSEGCTMSAKKDGLANIGGFLALREEAWVE
                     KLKNMLILIEGFPTYGGLAGRDLEAIAIGLREVLDESYLGFRIGQVTAFGEDLVGAGV
                     PVVEPIGGHAVYIDARAFAPHLEPEEYPAEALTIALYREGGIRAVEIGGVMFGKPDPE
                     RPGREILPDLDLVRLAVPRRVYTNSHLDYVADVVKRLHREPEKLRGVRIVRQAPVLRH
                     FTARFELV"
     gene            2665..3822
                     /locus_tag="VF958_01835"
     CDS             2665..3822
                     /locus_tag="VF958_01835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9147879.1"
                     /translation="MNRRLPEPVRFWRGRIDLARMKTLGKLLALLGLSLVVMASPREE
                     RFSSGRSRQIGNPAPVPKTVAGSTGLSPLATLSGGDFCGEPSPPTPITVLPFNDTGTT
                     IGKADNSMGSLPFACFGGEGSGPSRPGPDVFYSFTILGPGNGLTFSLTTTSNTYDPAI
                     YVLSACGNLDTCQGGVDAGVNGDPESLIVSNLPAGTYYLGVDSAYVFPDNSADGAYAL
                     SVTGSFGNTPTPTATPTETPTGTTPTPTPTVTPTQTPTPLSTFGFYTVAPCRVADTRD
                     PVGPFGAPPLQAGAIRTFTMTDRCSLPLNATAVSVNITVVVPTEAGYLTVFPAGSALP
                     LASTLNYGTNQVRANNAIVPLGTGGAISIFCGQGGGTTDVIVDVNGYFISP"
     gene            complement(3819..4388)
                     /locus_tag="VF958_01840"
     CDS             complement(3819..4388)
                     /locus_tag="VF958_01840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9147880.1"
                     /translation="MIPLSMILRTVSVIAFLGLAGPSPKPPLDFSGTWTLDEKASRGV
                     STNMHGAVLRVEQSGDHIRIFPVGRRAKLLGEEIVADGRPYEKALGPGRGVVTATWSK
                     DGASLWLEVTAGTQEDPRAAAAIQRSVWKLSPDGRTWLRHSVSMQGKELREARLVFRK
                     QERPNPNDGDLKSLRGAETRRRSRFSDGD"
BASE COUNT          768 a         1534 c         1425 g          784 t
ORIGIN      
        1 caacctagag attaagagtc tcttgctcta ccaattgagc tagcggccca ccgggacgcg
       61 gaagaataga cgatcgggtg cgagccggca agcgttcaca gggggcctct ggattcgcag
      121 cgcgaggagt ctcccgaagg gaggagcctc gctctgcagg agcaagcgag agcgacgact
      181 agccgcctcc gacgaactcg acgacttcga gcgcgtctcc ttccaagaga tgcgtggacg
      241 agtacgccga tttcggaacg atctcccggt tgcgctcaac cgctacgcgt ggcgtcgaga
      301 ctccgagagc gacgagcagc gcggcgatgg aggttccgtc gggaaacgtc cgctcgtcgc
      361 cgttcacgcg cactcggact tccatttggt ctcccgacga gcgacccccg tcggtcatac
      421 gcccctccgt accggatact ggatttcggt tgaaccggcc gcggaagtga gccgcacgac
      481 ggcggccgcc gtccccgtcg gaatcgtcgg catcaggaag gtgacttcgc agctgccatc
      541 gggacccgtc attccggcaa atacgggaac cggcttcgcg ctggaaagca ctcggatctg
      601 cacggaggct ccggcgacgg gacgtcgcga gagcgccagc tccgcccgca aacggacgtt
      661 gatccgtttg ccggaaacga actccggcgc cggcgagaac gtgagatcga tctgttcggg
      721 gtttccctcg gaggccaggt agtcgaggat cacctggtcg agacttcggt cccccgaaat
      781 gcgcgcggag tcgctcgggc gacggggaga aacgcgctga gccggccgct cccgcacctc
      841 cgagcccgcg cccgtggggg acgtgatccc accgagaacc atgtcgtccg ggacacgcac
      901 cgatccgtcc gccccctcga agctcccttc ccggaccgac gcgatcaggt cgcgatgctg
      961 gtgctccatc agctctcgga cggcgcgttc gtcggcgccg ttgcggagag actccggata
     1021 cgcggtccgc ttgccgccga gaatctcgcc gcccacgtag acgaaagact cgacgtacgg
     1081 gttttgatca ccgcggtcct ccgtctggat gtggaacacc cggtccttgt gcttgacgtc
     1141 ggtgttgtag ccggtgatca cgagaaaatt gtagccgctg gcgagcggcg aaactgctaa
     1201 accagctcga accgggccgt gaaatgccgc agcaccgggg cctggcgcac gatgcgcacg
     1261 ccccgcagct tctcgggttc ccggtgcagg cgcttgacga cgtcggcgac gtagtccagg
     1321 tgcgagttcg tgtacacccg tctcggcacc gccaggcgta cgagatcgag atccggaagg
     1381 atctcgcggc cgggccgctc cgggtccggc ttgccgaaca tgacaccgcc gatctcgacc
     1441 gcccggatcc cgccctcgcg gtaaagcgca atcgtcagtg cctcggccgg atactcctcg
     1501 ggctcgaggt ggggagcgaa ggcgcgcgcg tcgatgtaga ccgcgtgccc gccgatcggc
     1561 tcgaccaccg gcactccggc gccgacgagg tcctccccga acgcggtgac ttgcccgatg
     1621 cggaagccga ggtagctctc gtcgaggacc tcccggagac cgatcgcgat cgcctcgagg
     1681 tcgcggcccg ccaggccgcc gtaggtcggg aagccttcga tcaggatcag catgttcttc
     1741 agcttctcga cccacgcctc ttcgcgcagc gcgagaaaac cgccgatgtt cgccaatccg
     1801 tccttcttcg cgctcatcgt gcagccttcc gacagcgaga agatctcgcg cacgatctcg
     1861 gtgacgctcc gaccgtgctg tcccgcctcg cgctccttga tgaaccacgc gttctcggca
     1921 aagcggcaag cgtcgatgac gagcgggacg ccgtgcctcg cgcagaggtc gtgcgtcgcg
     1981 cggatgttct cgagcgaaac cggctgccct cccccggagt tgttcgtcag cgtcagcatg
     2041 acgagaggca cgcggccgcg gttctcctcg agacaacgct cgaggcgggc gagatccatg
     2101 ttgcccttga acggatgtcg gagtcgcggg gacttgcctt ccgggatcac gaagtcgagg
     2161 gcgatcccgc cgttgacctc gacgttggct cgcgtcgtgt cgaagtgggt gttgttgggc
     2221 acgacgtccc cgggcttcaa caccgtcgag aagaggaggt tctccgcgac ccgtccctgg
     2281 tgggtcggga tgacgtgggg gaggccgaag atttcccgga cggtcctctc gaagtggcga
     2341 tagtttcggc cgcccgcgta cgactcgtct ccgatcatca ggcccgccca ctgctcgtcc
     2401 gacatcgccg acgtgccgga gtccgtgagc aggtcgatgt agatgtcctc ggaatcgagc
     2461 ccgaagacgt tgtagcccgc tcggccgagc gcctcttcgc gctcctgcgg cgagatcgag
     2521 cggatccgct cgacgacctt gacgcgccac ggctccggcg acagctcacg catcccgcaa
     2581 gtgtattgcg cgacctcccc tcctcctgtg gcatcgctcc ttgacagctc cgtcatagtg
     2641 gtaggcgaaa acaggagaac agacgtgaat cgcagattgc cggagccggt gcggttctgg
     2701 cggggaagga ttgacctcgc gcggatgaaa accctcggaa aactcctcgc cctgctcggg
     2761 ctttctctgg tcgtgatggc ctcgccgcgc gaagagcgat tctcgtccgg tcgctcccgc
     2821 cagatcggca acccggcccc ggtgccgaag acggtcgctg gctcgactgg attatccccc
     2881 ctcgcgactc tcagcggcgg agacttctgc ggcgaaccgt cgcctccgac gccgattacg
     2941 gtgctccctt tcaacgacac cgggacgacg attgggaaag ccgacaacag catgggatcc
     3001 ctgccgttcg cctgcttcgg cggcgagggg agcgggcctt cgcggccggg tccggacgtc
     3061 ttctacagct tcacgattct cgggcccggc aacggcctga ccttctcgct gacgaccacg
     3121 tcgaacacgt acgatcccgc gatctacgtc ctctccgcct gcggaaacct ggacacgtgc
     3181 caaggcggag tcgacgccgg cgtcaacggc gacccggagt cgttgattgt ctcgaacctc
     3241 ccggcaggga cgtattacct cggcgtcgac tcggcgtacg tcttccccga taactccgcg
     3301 gatggggcct acgcgctgag cgtcacgggc tctttcggca acacgccgac gccgaccgcc
     3361 acgccgacgg agaccccgac gggcacgacg ccgacgccga cccccaccgt gacaccgacg
     3421 caaacgccga ctccactctc gacattcggt ttctatacgg tcgccccatg ccgcgttgcg
     3481 gacacgcgag atccggtcgg accgttcggt gctccgccgc tacaggcagg cgccatccgc
     3541 accttcacga tgacggaccg ctgcagtctt cctctgaacg ccaccgccgt ctcggtgaac
     3601 atcacggtcg tggttccaac cgaggcggga tatctcaccg tctttccggc ggggtcggcg
     3661 ctgccgctcg cctcgacgtt gaattacggg accaaccagg tccgggccaa caatgcaatc
     3721 gtccccctcg gaaccggagg ggccatttcg atcttctgcg ggcagggcgg cggaacgacg
     3781 gacgtgatcg tcgacgtaaa cggatacttc atttcgccct agtcgccatc cgagaaccgt
     3841 gaacggcgtc gagtctctgc gccgcgcagc gacttcaggt ctccgtcgtt gggatttgga
     3901 cgttcctgtt ttcgaaaaac gagtcgtgcc tctcgcagct ctttcccctg catgctcacg
     3961 ctgtgccgga gccacgtcct cccgtccggc gaaagcttcc agacgctgcg ttgaatggcc
     4021 gcggccgcgc gcgggtcttc ctgcgttccc gccgtcacct cgagccagag agatgcgccg
     4081 tccttggacc aggtcgcggt gacgacgccc cgtcccgggc cgagggcctt ctcgtacggc
     4141 cgaccgtcgg cgacgatctc ctcgccgagg agtttcgccc gccgtccgac ggggaagatc
     4201 cggatgtggt cgcccgactg ctccacccgc aggaccgcgc cgtgcatgtt cgtggagacg
     4261 ccacggctcg ccttctcatc gagcgtccag gttcccgaaa aatcgagagg cggcttcgga
     4321 gaaggcccgg cgagaccgag gaaagcgatg accgaaaccg ttcgcaggat cattgacagg
     4381 ggaatcatcg ggcagtagcg taatgcgcaa accggttttc cctcgtaagg agaaaagcat
     4441 gcgcgtgact tcgacacggc tcactcgggc ggtgctcctg gcacttttct cctccggaac
     4501 tctcctggcc g
//
LOCUS       DASWAV010000084         2730 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U2665
            k141_43189, whole genome shotgun sequence.
ACCESSION   DASWAV010000084 DASWAV010000000
VERSION     DASWAV010000084.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960316
            Sequence Read Archive: SRR10260007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2730)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2730)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:47:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,929
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2730
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_43189"
                     /isolate="SMAG_U2665"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.36444 N 132.31889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..712)
                     /locus_tag="VF958_01845"
     CDS             complement(<1..712)
                     /locus_tag="VF958_01845"
                     /inference="COORDINATES: protein motif:HMM:NF024491.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase D-like domain-containing protein"
                     /protein_id="HEX9147881.1"
                     /translation="MVPHNNAVLLDNGDATFAAILAAIRGARESINIELFIFNEGEIA
                     GQVARALADKAREGVEVRVLLDDFGSHPGPLEDLLTRAGVKLRWYKPLRIYTIYKVGK
                     RTHRKIMTVDGKIGFTGGFAVDDRWKGDARNPKEWRDTNVQVEGPVVAQLQAIFMEDW
                     VHTTGEVLNGQEQFPPIPAAGTMLVQAIASSRTDSSSKAKLLYYMAIQAARKTIWMEN
                     AYFVPDGQIRRGLVSAAAR"
     gene            985..2301
                     /locus_tag="VF958_01850"
     CDS             985..2301
                     /locus_tag="VF958_01850"
                     /inference="COORDINATES: protein motif:HMM:NF024491.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase D-like domain-containing protein"
                     /protein_id="HEX9147882.1"
                     /translation="MLRRKPRKPRTPRVYRISLEFYLIALAAAFAFSVAISLLNRPME
                     VAYEIPHSFGVRDPSFLASAQALDRPSMTAGNRVTILENGVQIFPGMLAAIHAAEKSV
                     NFESYIFWSDSVGRRFRDALAERARRGVEVRLLLDGVGTAKRLTRSDVEMLRSAGCRL
                     EYFRPIRPWMLDVINRRSHRRILVVDGKVGFTGGVGIAKEWAGNAESPEHWRDTVVRL
                     EGPAVSQMQSAFQDNWSEVTGELLVGEKFFPRLVPAGSVLAAVVPSSPRPSSSANQTL
                     YSVAIAAAEHTISISNSYFVPNDASIALLAAASRRGVDVRVLVPGAINDVPVTKAGGR
                     SKFGELLRAGVKIFEYQPAMMHAKTMVVDGIFATVGSTNFDNRSFRYNDEIDLAVYDE
                     GVAGRLQEMFDADVGKSRAYTYEQWIHRPPWKRFIELVVEPVRPQL"
BASE COUNT          465 a          910 c          855 g          500 t
ORIGIN      
        1 cgcgggcggc ggcgctgacg aggccgcgac ggatctggcc gtccgggacg aagtaggcgt
       61 tctccatcca gatggtcttt cgagccgcct ggatcgccat gtaatacagc agcttcgcct
      121 tcgagctcga gtcggtgcgc gacgaggcaa tggcctgaac gagcatcgtc cccgctgcgg
      181 ggatcggggg aaactgctcc tgcccgttca ggacctctcc ggtcgtatgg acccagtcct
      241 ccatgaagat cgcctggagc tgcgcgacca cggggccttc cacctggacg ttcgtgtcgc
      301 gccattcctt cgggttgcgg gcgtcgcctt tccaccgatc gtcgaccgcg aaaccgccgg
      361 tgaagccgat tttgccgtcc acggtcatga ttttccggtg cgtcctcttc cctaccttgt
      421 agatcgtgta gatccgcagc ggcttgtacc agcggagctt cacgcccgcc cgcgtcagca
      481 ggtcttcgag cggtccgggg tgggacccga agtcgtcgag caggacgcgc acctcgaccc
      541 cctcgcgggc cttgtcggca agagcacgcg cgacctgccc cgcgatctcc ccctcattga
      601 agatgaacag ctcgatgttg atgctctcgc gcgccccgcg gatggcggcg aggatcgcgg
      661 cgaaggtcgc gtccccgttg tcgagcagga ccgcgttgtt gtgcgggacc atctcggtcc
      721 cgaggacgtc gagcgagcga cggaactcgg cgctgtcgac gccgtaatcc ggcttgtacg
      781 tataggtggt ccgcttgtcc ccgaagagat acgaacatcc ggtcgaggga accagcgaga
      841 cgacgaggac gagcaggcgc agggcgcgca ggaggtatcg gttcaggaat tcacccggta
      901 tatctttgtc ttcgtgcgat tcagagtggc ccgttccacc ttcgcgggcc tccgcatgaa
      961 cacctcacct taaccaggct cccgatgctt cgaagaaaac ctcgaaaacc acgcacgccc
     1021 cgagtctacc gaatctcgct cgagttctac ctgatcgccc tcgcggccgc cttcgccttc
     1081 tccgttgcaa tttctctgct caaccgtccg atggaggtcg cctacgaaat cccccattca
     1141 ttcggcgtgc gcgacccgag cttcctggcg tctgctcagg cgctcgaccg cccgagcatg
     1201 accgcgggga atcgcgtcac gatcctcgaa aacggcgtcc agatcttccc gggcatgctg
     1261 gcggcgattc acgccgcgga gaaatcggtc aacttcgagt cctacatctt ctggagtgat
     1321 tccgtcggcc ggcgcttccg ggatgcgctc gcggagcgcg cccggcgcgg agtcgaagtc
     1381 cggctgctct tagatggcgt cggaacggcg aagcggctca cccggagcga cgtcgaaatg
     1441 ctgcggagcg ccggatgccg gctcgaatac ttccgtccca tccggccctg gatgctcgac
     1501 gtgatcaacc gccgcagcca tcgccgtatc ctggtggtcg acggcaaagt cgggttcacg
     1561 ggcggagtgg ggatcgcgaa agagtgggcg ggcaacgccg agtcgcccga gcactggcgg
     1621 gacaccgtcg tgcgactgga aggcccggcg gtctcccaga tgcagtcggc cttccaggac
     1681 aactggtcgg aggtgacggg cgaactgctc gtcggcgaga agttcttccc gaggctcgtc
     1741 cccgcgggaa gcgtcctggc ggccgtcgtt ccgtcgagcc cgcgtccgtc gagttccgcc
     1801 aaccagacgc tctattcggt cgcgatcgcc gcggccgagc acacgatctc gatctcgaac
     1861 tcctacttcg tcccgaatga cgcgtccatc gcgcttctcg ccgcggcgag ccgtcggggg
     1921 gtcgacgttc gggtgctcgt gcccggcgcg atcaacgacg ttcccgtgac caaggccgga
     1981 ggccgctcga agttcggaga gctgctgcgg gcgggtgtga agatcttcga gtaccagccg
     2041 gcgatgatgc acgcgaagac catggtcgtc gacgggattt tcgctaccgt cggatcgacc
     2101 aatttcgaca accggtcctt ccggtacaac gacgagatcg acctcgcggt ctacgacgag
     2161 ggggttgccg gtcgcctgca ggaaatgttc gacgccgacg tcggcaagtc gcgggcctac
     2221 acgtacgagc agtggatcca tcggccgccc tggaagcgct tcatcgagct cgtggtcgag
     2281 ccggtccggc cgcagctcta aggaggtagc ttccccgggg caattcagcg aagagtcgac
     2341 tccaaccttg gtccgatgac gatttaaaca atccttgtaa cgaaatctgc ggatggcgtg
     2401 acaatctccg tcattccgac cacccgccgc caacccgtaa gtactccaga caaaagactt
     2461 gctgatgggc tctgccgtgg cggaacgctt gctacttcaa accgccgagg aaccaaaaac
     2521 ctcaacctcc tcctttcgct ccccgggcgg ccccaccccg acccggggct ttttttcccg
     2581 gggcgggccc acccccgacc cgggagcttt ttccccgccg tattcgcaac cggtgccgcg
     2641 cttgccgaat ccccccgatc gcgggggatg atgagttgcc ggtgagccgc gggtctcccg
     2701 agcgctcgag gagacctgcc cgggagacaa
//
LOCUS       DASWAV010000085         2068 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U2665
            k141_203966, whole genome shotgun sequence.
ACCESSION   DASWAV010000085 DASWAV010000000
VERSION     DASWAV010000085.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960316
            Sequence Read Archive: SRR10260007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2068)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2068)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:47:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,929
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2068
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_203966"
                     /isolate="SMAG_U2665"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.36444 N 132.31889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..551)
                     /locus_tag="VF958_01855"
     CDS             complement(<1..551)
                     /locus_tag="VF958_01855"
                     /inference="COORDINATES: protein motif:HMM:NF019265.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEX9147883.1"
                     /translation="MIPRETQPGVDEARLIREVRAGNSEAFEYFVRQYQPKITRIAYR
                     LLKDVGEADCAAQESFLRAWQNLNEFREGSTFETWLTRICINWCKDRLKRRRVVLYFH
                     QAPDGGEDPGPRDVVSDSEPSPERRAQSREIRERLREAIEALSPRQKAVFTLKHFEEL
                     SIPEIAELTGLDSGTVKSHLFRAA"
     gene            complement(631..1266)
                     /locus_tag="VF958_01860"
     CDS             complement(631..1266)
                     /locus_tag="VF958_01860"
                     /inference="COORDINATES: protein motif:HMM:NF015648.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PH domain-containing protein"
                     /protein_id="HEX9147884.1"
                     /translation="MAYAEKNLAPGESIVYRARYHWIYYRTTLALLLVAAVSGLWWWI
                     SGQRLQSGAASSIFGTIALVLFLIALVHFLLRRIRASADEFVVTTRRVIRKTGLVARE
                     AEHAPIEKIQDISIDQGVIARMLGYGTAVIETASESGRIVFPDIADPEKFRSAIWGQT
                     TGGASAAAITATTVPQPNVASRLAELEDLRTRGLLTTEEYARKRQEIVASP"
     gene            1304..>2068
                     /locus_tag="VF958_01865"
     CDS             1304..>2068
                     /locus_tag="VF958_01865"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005869545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX9147885.1"
                     /translation="MARPQVIFVTGCSTGIGRATAIEAARRGHRVFASARRRQDIADL
                     TAKGIETLTLDVTDTGSIAEAVTAVLATAGGIDALVNNAGYGQYGAVEDVSPEEWRAQ
                     FDVNVFGALAVLRAVLPAMREARRGTVVNVSSVAGKVTVPFAGAYCASKHALEAISDA
                     LRVELAPWQIRIVVIEPGPIGTRFGDRTRQATARILSTPGPYSRFYANAEKASERGFQ
                     RGRRRPEAVARVIVGAIESKRPRTRYRVAPLARVLVP"
BASE COUNT          323 a          702 c          715 g          328 t
ORIGIN      
        1 gccgcccgaa agaggtggga cttgacggtg ccggagtcga gcccggtgag ctccgcgatt
       61 tccggaatcg agagctcttc gaagtgcttc agcgtgaaca cggccttctg ccggggcgaa
      121 agggcctcga tcgcctcccg cagccgctcg cgaatctcgc gcgactgcgc ccgccgctcc
      181 ggcgaaggct ccgagtccga aaccacgtcg cgcggaccgg gatcttcccc gccatccggc
      241 gcctggtgaa agtagaggac gacccggcgg cgcttcagcc gatccttgca ccagttgatg
      301 cagatcctcg tcagccatgt ctcgaacgtc gacccctctc gaaactcgtt caagttctgc
      361 caggcccgca gaaagctctc ctgggcggca cagtccgcct cgccgacgtc tttcaggagg
      421 cgataggcga tccgcgtgat cttcggctgg tactgccgga cgaagtactc gaacgcctcc
      481 gagttgccgg cgcgaacctc ccggatgagg cgcgcttcgt cgacgcccgg ctgcgtctcc
      541 cgaggtatca cggccagagc cggcactccc gatagacgag cggggagcga atgggtttac
      601 cgatccggct cgctccccgg gcggccggtt tcatggcgac gccacgatct cctggcgttt
      661 tcgcgcgtac tcctcggtcg tcagcagccc gcgcgtgcgc agatcttcca gctccgcgag
      721 gcgggaggcc acgttgggct gcggcaccgt tgttgccgtt atggccgcgg ccgacgcgcc
      781 acccgtggtc tggccccaga tcgcgctgcg gaacttctcg gggtccgcga tgtccggaaa
      841 cacgatcctt cccgactccg acgcggtctc gatgacggcg gtgccgtagc cgagcatccg
      901 ggcgatcacc ccctggtcga tcgagatgtc ctggatcttc tcgatcggcg cgtgctcggc
      961 ttcgcgcgcc acgagtcccg tcttgcggat gacccgccgc gtcgtcacga cgaactcgtc
     1021 ggcggacgcg cggatcctcc ggagcaagaa gtgaacgagc gcgatgagga agagaacgag
     1081 agcgatcgtg ccgaagatcg acgacgcggc gccggattgc aggcgctgcc cggagatcca
     1141 ccaccagaga ccggacacgg cggcgacgag cagcagggcc agggtcgtgc ggtagtagat
     1201 ccagtggtac cgggctcggt agacgatcga ctcacccggc gcgaggtttt tctccgcgta
     1261 ggccatcgga tgcgacctcg gagagtaaca tcgccgatcg gggatggctc gcccgcaggt
     1321 gattttcgtg accggctgct cgaccgggat cggccgggcg accgcgatcg aggcggcgcg
     1381 gcgggggcac cgcgtgttcg cgtcggcacg ccgccggcag gacatcgcgg acctgaccgc
     1441 gaaggggatc gagacgctca ccctcgacgt caccgacacc gggtcgatcg ccgaggcggt
     1501 gacggcggtc ctcgcaaccg cgggtgggat cgacgccctc gtcaacaacg cgggctacgg
     1561 gcagtacggc gcggtcgagg acgtctcgcc ggaggagtgg cgcgcccagt tcgacgtgaa
     1621 cgtcttcggc gctctggcgg ttctccgcgc ggttcttccc gcgatgcgcg aggcacgccg
     1681 ggggaccgtg gtgaacgtct cctccgtcgc cggaaaggtc acggtgccct tcgcgggggc
     1741 ctactgcgcc tccaagcacg cgctcgaggc catctccgac gctctgcggg tcgagctcgc
     1801 gccgtggcag atccggatcg tcgtcatcga gccgggcccg atcggaacgc gcttcgggga
     1861 tcgcacccgt caggcgaccg cacgcatcct gtcgacgccg gggccttatt cgcgcttcta
     1921 cgcaaatgcg gaaaaggcga gcgagagggg gttccagcgg ggacgccgcc ggcccgaggc
     1981 agtggcccgg gtgatcgtcg gggcgatcga atcgaagcga ccgaggactc ggtaccgggt
     2041 cgctcctctc gcacgggtcc tcgtcccg
//
LOCUS       DASWAV010000086         4336 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U2665
            k141_244556, whole genome shotgun sequence.
ACCESSION   DASWAV010000086 DASWAV010000000
VERSION     DASWAV010000086.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960316
            Sequence Read Archive: SRR10260007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 4336)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4336)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:47:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,929
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4336
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_244556"
                     /isolate="SMAG_U2665"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.36444 N 132.31889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1636)
                     /locus_tag="VF958_01870"
     CDS             complement(<1..1636)
                     /locus_tag="VF958_01870"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX9147886.1"
                     /translation="MAEERILIVDDDEDILLIVQTILANAGYSVTLARNGREGVDLAL
                     ELKPDLILLDVMMPELSGWEVCTTLKNAPETRQTPIVMMTVKSEIRDLITGMQVGADD
                     YITKPFTRRKLLATVRQQIEQRGETSSAFLAGENEQVRFKSLLFDSVTDLPTVPVIID
                     ALRDRLLDSRDLAVLYIDVEQYSHIEDTYGWEVFDGLLRHTAKALRRMLGTVFAAEDF
                     VAVNRTGGSDFYVFTSLEGGDEAGARLQRKARQVEESLRGTLDEAFGGRIHKRIAVFV
                     GHAIIRSNPQMRVERLVYRALREAISIATTKERERQAVLADAFREIVRKKRIRTVYQP
                     IFDLNTMELFGHEALTRGPLDSAFESPDLLFQFAGEREATWELEQLCLDSSASHYASA
                     PGSLLFLNVEADSIASLSTRGVQALAPLLGLRYPVVLEVTERSAIRDLPVFRDSLAML
                     REHGFRIAIDDAGSGYASLQSIAELRPNFLKVASTLVTGLHEDTIKRDVVEMQHRLAF
                     DELFALQLSAALIRKEWEAEQVGHRFSIKKYEDKIDT"
     gene            complement(1684..2196)
                     /locus_tag="VF958_01875"
     CDS             complement(1684..2196)
                     /locus_tag="VF958_01875"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020249673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003999 - adenine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HEX9147887.1"
                     /translation="MDLRRFIRDVPDFPTPGILFRDITPLLSSPEAFRAAVDAMVAPF
                     RDAGAGKVVGIEARGFMFGAAVARELGLGFVPARKPGKLPGKTERVAYGLEYGSDALE
                     VHADAFSPGESVLIVDDVLATGGTSAAAAELVERLGARVTALVFCIELAALQGRSRLS
                     GRPAHAVLVL"
     gene            complement(2196..2465)
                     /locus_tag="VF958_01880"
     CDS             complement(2196..2465)
                     /locus_tag="VF958_01880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014133424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HEX9147888.1"
                     /translation="MIARRAVVSGRVQGVGFRFFAERVARASGVRGWVRNLPDGRVET
                     VIEGEEDAVARYLEKVGGGPFGGSVSGFDVEEREPEGFERFEITR"
     gene            complement(2462..3136)
                     /locus_tag="VF958_01885"
     CDS             complement(2462..3136)
                     /locus_tag="VF958_01885"
                     /EC_number="2.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008871723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004719 - protein-L-isoaspartate
                     (D-aspartate) O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate(D-aspartate)
                     O-methyltransferase"
                     /protein_id="HEX9147889.1"
                     /translation="MPLSERLSDGARADAQRREMVERYVAARGVDDTRVLAAMLEVPR
                     HRFVPEPLRAKAYGDHSLPIGHGQTISQPSVVGLMTQRLAVQPGQKVLEIGTGSGYQA
                     AVLSRIARTVYSLERIDELARAAAQAIAELGYQNVSVKAFDGTYGYPAAAPFDRIIVT
                     AGTAEIPDTLLAQLAVGGRLVAPVGPPERQRIRVVRRRKTDFAREDGEEVVFVPLIGR
                     FGRAPA"
     gene            3297..>4336
                     /locus_tag="VF958_01890"
     CDS             3297..>4336
                     /locus_tag="VF958_01890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010418745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent dehydrogenase
                     E1 component subunit alpha"
                     /protein_id="HEX9147890.1"
                     /translation="MAGISREELKKWYALMHLGRVLDDKAPNYLKQGLGWSYHAPCAG
                     HDAIQLALGMTFRPGRDYLFPYYRDLTTCVAAGLTAEEILLNGMSKATDVASGGRHMS
                     NHFAKSEIGIQNVSSCVANHAAHAAGLGRAIKTYGRDSIVFCSLGESSTSEGYFYEAL
                     NGASREKLAVVFVIQDNGYGISVPKIDQTANPTVADNYSGLLNLRIVHCDGLDVFDSF
                     RAMREAVDYVRTGQGCAIVYAPCVRIHSHSNSDRHELYRSPEELEAAREADPLPRFRR
                     TLIDGGLLSEEEIVAIEAHNQQTYEDAADRARRAPDPTPESIFEYVAPEPWTPDGWPE
                     GIPDGTGEAMTLV"
BASE COUNT          763 a         1467 c         1390 g          716 t
ORIGIN      
        1 aagtatctat tttatcttca tattttttta tagaaaaacg atgaccaact tgttctgctt
       61 cccactcttt tcgtataagg gcagcagaca actggagtgc aaataattca tcaaatgcta
      121 accggtgttg catctcgacg acgtcacgct tgatcgtgtc ctcgtgcagg cccgtcacga
      181 gcgtgctcgc gaccttgagg aagttgggcc gcagctctgc gatggactgc agcgaggcgt
      241 aaccggagcc ggcgtcgtcg atcgcgatcc ggaagccgtg ctcccgaagc atcgcgagcg
      301 agtcgcgaaa aaccggcagg tctcgaatcg cggagcgctc ggtgacctcc aggacgaccg
      361 gataccgcag gcccaggagc ggcgccagcg cctggacgcc tcgcgtcgaa agtgacgcga
      421 tcgagtccgc ctcgacgttc aggaaaagca ggctcccggg agcggaggcg tagtggctgg
      481 cggacgagtc gagacagagt tgctcgagct cccaggtcgc ctcgcgctcg ccggcgaact
      541 ggaacaggag atcggggctc tcgaaggcgg agtcgagggg cccgcgcgtc aaggcctcgt
      601 gcccgaagag ctccatggtg ttcagatcga agatcggctg gtagaccgtg cggatgcgct
      661 ttttgcgcac gatctcgcgg aacgcgtcgg cgaggaccgc ctgccgctcg cgctccttcg
      721 tcgtggcgat cgaaatcgcc tctctcagag cgcggtagac gagtctctcc acgcgcatct
      781 gcgggttcga acggatgatg gcgtgcccga cgaaaacggc gatccgtttg tggatccggc
      841 cgccaaacgc ctcgtccagc gtgccccgca gcgactcctc cacctgccgg gccttgcgct
      901 ggaggcgcgc cccggcttcg tctccccctt cgagactcgt gaacacgtaa aagtccgaac
      961 cgccggtgcg gttcaccgcg acgaagtctt ccgccgcgaa cacggttccg agcatccgcc
     1021 gcaacgcctt cgccgtgtgt cgcagcaggc cgtcgaacac ctcccagccg taggtgtcct
     1081 cgatgtggct gtattgctcg acgtcgatgt agaggacggc gagatcgcgg ctgtccagaa
     1141 ggcgatcacg caacgcgtcg atgatgaccg gaacagtcgg caggtccgtg acgctgtcga
     1201 acagcagact cttgaagcga acctgctcgt tctcccccgc caggaacgcc gacgaggttt
     1261 ccccccgctg ctcgatctgc tggcggacgg tcgccaggag ctttcgccga gtgaagggct
     1321 tggtgatgta gtcgtcggcg ccgacctgca tcccggtgat gaggtcgcgg atctcgctct
     1381 tgaccgtcat catcacgatc ggcgtctgcc gcgtctccgg cgcgttcttc aacgtcgtgc
     1441 agacttccca ccccgagagc tcgggcatca tgacgtcgag caggataagg tcgggcttca
     1501 actcgagcgc caggtcgacg ccttcccggc cattgcgcgc gagcgtgacg gaatagccgg
     1561 cgttggcgag gatcgtctgc acgatcaaca gaatgtcctc gtcatcgtca acgatgagga
     1621 tgcgttcttc ggccatctat gtgctgactt ggttgtcggt tcggccgggt cggacgctcc
     1681 ccttcatagt accagcaccg cgtgcgcggg gcgaccggaa aggcgcgacc gcccctgcag
     1741 cgcggcaagc tcgatgcaga agacgagcgc cgtcacccgc gcccccagcc gctcgacgag
     1801 ctccgcagcg gcggccgagg tgccgccggt cgcgaggacg tcgtcgacga tcaggacgga
     1861 ctcccccggc gagaaggcat cggcgtggac ctccagcgcg tcggacccgt actccaggcc
     1921 gtaggcgact ctctcggtct ttcccggaag ctttcccggc tttcgcgccg gcacgaagcc
     1981 gaggccgagt tcccgcgcca ccgcggcgcc gaacatgaat cctcgagcct caatcccgac
     2041 gaccttcccg gcacccgcgt cgcgaaacgg cgccaccatg gcgtcgaccg ccgcgcgaaa
     2101 cgcctctggc gacgagagga gcggcgtaat gtcgcggaag aggatgccgg gcgtcggaaa
     2161 gtccggcacg tcccggatga accggcgcag atccatcaac gggtgatttc gaatcgctcg
     2221 aaaccctcgg gctcccgctc ctcgacgtcg aatcccgaga cgcttccgcc gaacgggccg
     2281 ccacccacct tctccagata tcgggcgacg gcatcttcct cgccttcgat cacggtttcg
     2341 acgcggccat ccgggagatt gcggacccaa ccgcgcacgc ccgacgcccg ggcaacgcgt
     2401 tccgcgaaga agcgaaatcc cacaccctgg acgcggcccg agacgaccgc gcgccgcgcg
     2461 atcacgcggg cgcccgtccg aagcggccga tcagcgggac gaacacgacc tcctcgccgt
     2521 cttcgcgcgc gaagtccgtt ttccgacgcc gcacgacgcg gattctctgc cgttccggag
     2581 gacccaccgg cgcgacgagc cgtcccccga cggccagctg cgcgagcagc gtgtccggaa
     2641 tctccgcggt gccggccgtc acgattatcc gatcgaacgg cgcggccgcg ggatatccgt
     2701 acgtcccgtc gaaagccttc accgagacgt tctggtaccc gagctccgcg atcgcttgag
     2761 ccgcggctcg tgcgagctcg tcgatccgct cgagcgagta cacggtgcgt gcgatccgcg
     2821 agaggaccgc cgcctggtaa ccggacccgg tgccgatctc gaggaccttc tggccgggct
     2881 gcacggcgag gcgctgggtc atcaggccga cgaccgacgg ctgcgagatg gtctggccgt
     2941 ggccgatcgg cagagagtgg tcgccgtagg ccttggcgcg gagaggctcg ggaacgaaac
     3001 ggtgtcgcgg aacctcgagc atcgcggcga gaacgcgcgt gtcgtctacg ccgcgggcgg
     3061 cgacgtagcg ctccaccatc tcccggcgct gcgcgtccgc gcgggcgccg tcggaaagcc
     3121 gctcgctcag gggcactggt gagatggtag caaggtggga gaagccccgg acaccgcccg
     3181 aggcacttcc gggccggtaa ttcgaataaa accggctaat attggggttt gactttcctc
     3241 gtggagggac gaaaccgttg acgcctcgag aaaccgctga gctcgttccg cccgccgtgg
     3301 cgggcatctc ccgggaggag ctcaagaagt ggtacgcgct catgcacctc gggcgggtgc
     3361 tcgacgacaa ggcgcccaat tacctgaaac aggggctcgg ctggagctac cacgccccct
     3421 gcgccgggca cgatgccatc cagctcgcgc tcggcatgac gttccgtccg ggacgggact
     3481 acctctttcc ctactaccgc gacctgacga cgtgcgtcgc agccggtctg acggccgagg
     3541 aaatcctctt gaacggcatg tccaaggcga ccgacgtcgc gtcgggcggc cggcacatgt
     3601 ccaaccactt cgcgaagtcg gagatcggca ttcagaacgt ttcctcctgc gtcgccaatc
     3661 acgcggctca cgctgccggg ctcggtcgcg ccatcaagac ctatggccgg gactcgatcg
     3721 tcttctgctc gctcggtgag tcttccacgt cggagggtta cttctacgaa gccctcaacg
     3781 gagcgtcccg cgagaagctc gccgtcgtct tcgtcatcca ggacaacggc tacggcatct
     3841 ccgtgcccaa gatcgaccag accgccaacc ccacggtcgc cgacaactat tcggggctgc
     3901 tcaatctccg gattgtgcac tgcgacgggc tcgatgtctt cgactccttc cgcgcgatgc
     3961 gcgaggccgt tgactacgtt cggaccgggc agggatgcgc gatcgtctac gcgccatgcg
     4021 tgcggatcca ctcgcactcg aactcggatc gccacgagct ctatcggtca cccgaggagc
     4081 tggaggcggc ccgcgaagcc gatccgctgc cgcgattccg gcggacgctc atcgacgggg
     4141 ggctcctctc ggaggaggaa atcgtcgcga tcgaggccca caaccagcag acctacgagg
     4201 atgcagccga ccgcgcgcgg cgagcgccgg atccgacgcc ggagtcgatc ttcgaatacg
     4261 tggcgcccga gccatggacg cccgacggct ggccggaggg aatccccgac gggaccggcg
     4321 aagcgatgac tctcgt
//
LOCUS       DASWAV010000087         1958 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U2665
            k141_445088, whole genome shotgun sequence.
ACCESSION   DASWAV010000087 DASWAV010000000
VERSION     DASWAV010000087.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960316
            Sequence Read Archive: SRR10260007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 1958)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1958)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:47:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,929
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1958
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_445088"
                     /isolate="SMAG_U2665"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.36444 N 132.31889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..606
                     /locus_tag="VF958_01895"
     CDS             <1..606
                     /locus_tag="VF958_01895"
                     /inference="COORDINATES: protein motif:HMM:NF018652.4"
                     /note="catalyzes the oxygen-independent formation of
                     protoporphyrinogen-IX from coproporphyrinogen-III; Derived
                     by automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase"
                     /protein_id="HEX9147891.1"
                     /translation="EPKLPSERDKFEIFRTALSRFSDAGYEYIGMDHFARPDDELSRA
                     RRERTLHRNFQGYTTKAGTDLLGLGMSAIGAIRDSFVQNRRDLPGYRAAVESEGAATF
                     RGFHLSFDDRVRRAVIESMLCHGVVVIPEIEATYGIRFREYFADALERLAPCADDGLV
                     EISDSELRATPLGRVFLRNLAMPFDAYLPAQLEKPVFSRTL"
     gene            715..1695
                     /gene="hemH"
                     /locus_tag="VF958_01900"
     CDS             715..1695
                     /gene="hemH"
                     /locus_tag="VF958_01900"
                     /inference="COORDINATES: protein motif:HMM:NF012964.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004325 - ferrochelatase activity
                     [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrochelatase"
                     /protein_id="HEX9147892.1"
                     /translation="MSDRAILFLQLGGPENLDEVPGFLYRLFADPDVIRVKPAILRKT
                     IAASIALARKGASRELYRSIGGGSPIRRWTEAQSAGVEERLRASGREVVVRAAMNCSN
                     PMVEDVVRDLASRGVRRFLALPLYPHYSLTTTKGALERSRAAVRAFAPQASFHEVGSW
                     PTHPGFVAAHAEAIREELARFSDPRPEKVHLLYSAHSIPKKLVTREGDPYPREVEATI
                     AAINASLGGRSPWSLAYQSKLGPVEWLGPPTLEAIRELGRQRVSQVLVIPIAFVSDHV
                     ETLYEVDQLFGEEAKQAGIEQFRRTPGLNDRPTFLAALADIASSNAEFWR"
     gene            1692..>1958
                     /locus_tag="VF958_01905"
     CDS             1692..>1958
                     /locus_tag="VF958_01905"
                     /inference="COORDINATES: protein motif:HMM:NF013739.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEX9147893.1"
                     /translation="MTTAVVGGGLSGLVRARALAARGEQVLLFEESDHPGGVVRSANQ
                     DGYLLELGPNSVRPTPELWGLVEELGLESEALLANPSMPRFIDFG"
BASE COUNT          303 a          680 c          639 g          336 t
ORIGIN      
        1 gagccgaagc tcccgtcgga gcgcgacaag ttcgagatct tccggaccgc gctttcacgg
       61 ttctccgacg ccgggtacga gtacatcggc atggatcact tcgcccggcc ggacgacgag
      121 ctctcccgcg cccgacgaga gaggacgctg caccgcaact tccaggggta tacgacgaag
      181 gcgggaaccg acctgctcgg gctcgggatg agcgcgatcg gcgcgatccg cgattctttc
      241 gtccagaacc ggcgtgacct cccgggttac cgcgctgccg tggagtccga gggagcggcg
      301 accttccgtg gcttccacct ctctttcgac gaccgcgttc ggcgcgccgt catcgagagc
      361 atgctctgcc acggagtcgt cgtgatcccc gagatcgagg cgacctatgg aatccgtttc
      421 cgcgaatatt tcgccgatgc gctggaaagg ctcgcgccct gcgccgacga cgggttggtc
      481 gagatctccg attcggagct tcgagcgacc ccgctcggcc gggtcttcct gcgcaacctt
      541 gccatgccct tcgatgccta tctcccggcg cagctcgaga agcccgtctt ttcccggact
      601 ctgtagcgcg gcggcgcaag atacagccgg tcacggcggc cgcgcgtccg cgccgccgca
      661 aagagagttt ctaaattacc cagactgcag gcctgtaccc gataaagtga gagcgtgagc
      721 gacagagcga tcctttttct ccagctcgga ggccccgaga acctcgacga ggttccgggg
      781 tttctctatc ggttgttcgc cgaccccgac gtcatccgcg tcaagcccgc gatcctgcgc
      841 aagacgatcg ccgcatccat cgcgctcgca cgcaagggcg cttcacggga gctctaccgc
      901 tcgatcggcg gcggctcgcc gatccggcgc tggacggagg cccagtccgc cggcgtcgaa
      961 gagcgcctcc gcgcgtcggg gcgcgaggtg gtggttcgcg ccgcgatgaa ctgctcgaat
     1021 ccgatggtcg aggacgtcgt ccgcgatctc gcttcccggg gcgtccgccg atttctcgct
     1081 ctgcccctct acccccacta ttcgctgacg acgacgaagg gcgcgctcga gcgctcacgc
     1141 gcagccgtgc gcgcctttgc gccgcaggcc tcgttccacg aggtcggatc ctggcccacg
     1201 caccccggct tcgtcgctgc gcacgcggag gccatccgcg aagagctcgc ccggttttcc
     1261 gatccgcggc cggagaaggt ccaccttctg tactccgcgc actcgatccc caagaagctc
     1321 gtcacgcgcg agggggaccc gtatccacgc gaggtggagg cgacgatcgc tgcaatcaac
     1381 gcctcactgg gcggccggtc gccgtggtcg ctggcgtacc agtcgaagct cggaccggtc
     1441 gagtggctcg gccctccgac gctcgaagcg atccgcgaac tcggccggca gcgagtgtca
     1501 caggttctcg tgatcccgat cgccttcgtg agtgaccacg tcgagacgct ctacgaagtc
     1561 gaccagctgt tcggagaaga ggcgaaacag gcgggtatcg agcagttccg tcgaacgccc
     1621 ggtctgaacg atcggccgac gttcctcgcg gcgctcgccg acatcgcgtc ctcgaacgcg
     1681 gagttctggc gatgacgacc gcggtggtcg gaggcggcct ctccgggctc gtccgggcgc
     1741 gcgccctcgc cgcgcggggc gaacaggtgc tcctcttcga ggagtcggac catccgggcg
     1801 gggtggtgag atccgccaac caggacggct atctcctcga gctcggtccg aacagcgtgc
     1861 ggccgacgcc ggagctgtgg gggctcgtcg aggagctggg gctcgagtcg gaggctctcc
     1921 tcgccaaccc ctcgatgccg cgtttcatcg acttcggg
//
LOCUS       DASWAV010000088         2401 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U2665
            k141_124689, whole genome shotgun sequence.
ACCESSION   DASWAV010000088 DASWAV010000000
VERSION     DASWAV010000088.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960316
            Sequence Read Archive: SRR10260007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2401)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2401)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:47:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,929
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2401
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_124689"
                     /isolate="SMAG_U2665"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.36444 N 132.31889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..178)
                     /locus_tag="VF958_01910"
     CDS             complement(<1..178)
                     /locus_tag="VF958_01910"
                     /inference="COORDINATES: protein motif:HMM:NF044910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ChrB protein"
                     /protein_id="HEX9147894.1"
                     /translation="MPAKDRTGAWYLIVHQVPPRPLYLRAKIRQRLAKVGAVALKDAV
                     YVLPRREDCLEDFEW"
     gene            269..1435
                     /locus_tag="VF958_01915"
     CDS             269..1435
                     /locus_tag="VF958_01915"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX9147895.1"
                     /translation="MDAFGLERNVVAVAAAMFLLGLGENLWKSFLPKYLQALGAPVLA
                     IGLYGTLRDFVDGAYQYPGGWIADRWGRRRALLLFVGLTSIGYIIYAVAPSWPFVLAG
                     LFFVMAWSSMASPTLFAVVGDSLPRGRRAIGFTVQSILKRLPIVIAPTLGGLTIAALG
                     VRHGVRAALVVTLALALVTLAVVARVRIPVQRETTPTGIGGVWRSLPAPLKWLLLSDV
                     FVRACEGLVDVFLVVYATNVIGVSAPRYGALIAIQMATAILVYVPASRLADRAGRKPF
                     VIATFVCFALFPIAVVLSRTFPALAGAFVVGGLREVGEPARKAMIVDLCRPEIRARSI
                     GLYYLVRSAAIAPAAFTGGLLWKIDPAIPFFVAGVVGIAGTVLFAATVEERFAA"
     gene            1473..2366
                     /locus_tag="VF958_01920"
     CDS             1473..2366
                     /locus_tag="VF958_01920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006891542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1259 domain-containing protein"
                     /protein_id="HEX9147896.1"
                     /translation="MICPLLALLLLVAPAAGQSAGRPDPASIEKLTGARGAWNEKEGV
                     FKVSVPRADLAVTARGVRITPALGLTSWAAFSGSGAHSMVMGDMVLTEDQVSHVMRVA
                     LDGGLEVTGLHNHFLGETPRVMFLHVGGAGEEAALAATIGKVFVTIREMSPARTEFRE
                     PSIDPASTSLDSAKLDALLGQKGELKDGVYKIVVGRTVRVHGADAGAAMGVNTWAAFA
                     GSAERAVVDGDFATLASELQPVLKALTGAGIDVVAIHNHLTGEEPRIVFLHYWGIGRA
                     EDLARGVRAALDLSRSSSALR"
BASE COUNT          338 a          785 c          831 g          447 t
ORIGIN      
        1 tccactcgaa gtcctcgagg cagtcctccc ggcgggggag gacgtagacg gcgtccttga
       61 gcgcgacggc gcccaccttt gcgagcctct ggcgaatctt cgcgcgcagg tagagcggcc
      121 gaggcggaac ctggtggacg atcagatacc aggcgccggt gcgatccttc gcgggcacag
      181 gagtgagtct atcagcagct agattggata gagcaactgc tattattcat gtatgtcgag
      241 cccggcgcgt cggcgttcgg agttcttcgt ggacgctttc ggtctcgagc gcaacgtcgt
      301 cgctgtcgcc gcggcgatgt tcctcctcgg actcggagag aacctctgga agagcttcct
      361 cccgaaatac ctccaggccc tcggcgcgcc cgtgctcgcg atcggccttt atggaacgct
      421 gcgcgatttc gtggacgggg cgtaccagta tccgggcggg tggattgccg accgatgggg
      481 gcgcagacgc gcgctccttc tcttcgtcgg gctcacctcg atcggctaca tcatctacgc
      541 ggtcgcgcct tcgtggccct tcgtgctcgc gggccttttc ttcgtgatgg cgtggtcgag
      601 catggcgagc ccgacgctct tcgcggtcgt gggcgattcg cttccgcgcg ggcggcgcgc
      661 catcgggttc accgtgcagt ccatcctcaa gcggctgccg atcgtgatcg cgccgactct
      721 cggaggtctg acaatcgcgg ctctcggcgt gcgccacgga gtacgggccg ccctcgtcgt
      781 cactctcgcg ctggcgctcg tgaccctcgc ggtggtcgcc cgggtccgga ttcccgtgca
      841 acgcgagacc acgccgaccg gcatcggcgg cgtgtggcgc tcgcttcccg ctccgctcaa
      901 gtggctgctc ctctccgacg tgttcgtccg tgcctgcgag gggctcgtcg acgtgttcct
      961 cgtggtctac gcgacgaacg tgatcggcgt gagcgcgccc cggtacggcg ccctcatcgc
     1021 aatccagatg gcgacggcga tcctcgtcta cgtgccggcg tcgaggctcg ccgaccgcgc
     1081 cgggcgaaag ccgttcgtca tcgcgacgtt cgtctgcttt gccctcttcc cgatcgcggt
     1141 cgtcctctcg aggacctttc ctgctctcgc cggcgcgttc gtcgttgggg ggctgcgcga
     1201 ggtgggcgag cccgcgcgca aggctatgat cgtcgacctg tgccggccgg agatccgcgc
     1261 tcgctcgatc gggctctact atctcgtccg cagcgccgcg atcgcgcccg cggctttcac
     1321 gggagggctc ctgtggaaga tcgaccccgc gatcccgttc ttcgtcgcgg gagtggttgg
     1381 aatcgcggga accgtgctct tcgccgcgac cgtcgaggag cgtttcgccg cctgaggaat
     1441 gccaactgtc tgcaattgac cggaggtcca tcgtgatctg ccctcttctc gccctgctgt
     1501 tgctcgtcgc gcccgctgcc ggacagtccg ccggacgtcc ggacccggct tccatcgaga
     1561 agttgacagg agcaagaggg gcgtggaacg agaaggaggg cgtcttcaag gtctccgttc
     1621 cgcgggcgga cctcgcggta acggcgcggg gagtccggat cacgcccgcg ctcggcctga
     1681 cgtcctgggc cgcgttctcc ggctcgggcg cgcattcgat ggtgatgggt gacatggtcc
     1741 tgaccgagga ccaggtgagc cacgtcatga gagtcgcgct ggatggcggg ctcgaggtca
     1801 ccgggctgca caaccatttc ctcggcgaga ctccgcgggt gatgttcctc cacgtgggag
     1861 gcgcgggaga ggaagccgcc ctcgcggcga cgatcggaaa agtcttcgtg acgatccgcg
     1921 agatgtcacc ggcccggacg gagttccggg agccgtcgat cgatccggcg agcacttcgc
     1981 tcgattccgc aaagctcgat gccttgctcg ggcagaaggg cgagctcaaa gacggggtct
     2041 acaagatcgt cgtcgggcgc acggtccggg tccacggcgc ggatgcgggc gcggcgatgg
     2101 gagtcaacac gtgggcggct ttcgcagggt cggcggagcg cgcggtcgtg gacggggact
     2161 tcgcgacgct cgcgtcggag ctgcagcccg tcctgaaggc gttgaccgga gccggaatcg
     2221 acgtcgtcgc catccacaac cacctgacgg gcgaggaacc ccggatcgtg tttctccact
     2281 actgggggat cggccgcgcc gaggatctgg ccagaggagt gcgcgccgcg ctcgacctca
     2341 gcaggagctc gagtgctctg cgctgagcga aggacactcg cgaccagagc ttagagctcg
     2401 a
//
LOCUS       DASWAV010000089         4232 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U2665
            k141_485360, whole genome shotgun sequence.
ACCESSION   DASWAV010000089 DASWAV010000000
VERSION     DASWAV010000089.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960316
            Sequence Read Archive: SRR10260007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 4232)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4232)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:47:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,929
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4232
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_485360"
                     /isolate="SMAG_U2665"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.36444 N 132.31889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1072)
                     /gene="lysA"
                     /locus_tag="VF958_01925"
     CDS             complement(<1..1072)
                     /gene="lysA"
                     /locus_tag="VF958_01925"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: protein motif:HMM:TIGR01048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008836 - diaminopimelate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HEX9147897.1"
                     /translation="MTGFSREDGELACDGVALSAIAREVGTPAYVYSGALIAENYRRF
                     DDAFADVRHLVCYAAKANSNLALLRRLAELGAGADVVSAGELRAVLGSGFPADRVVFS
                     GVGKTDEEIRFAVENELLAINAESEREIEKIEAAAAAGVTARVALRVNPDIDARSHPY
                     ISTGRRHNKFGVEIGRAREIFSRARRLEHVRMTGVQAHIGSQILDPEPLAQSARDLAG
                     LAVALASDGHPIETVDAGGGIGIAGEGPEALTPERYAAAVLPHFRDLAFKILIEPGRA
                     IVGPAGALLTKVLYVKENSGRLFVITDAGMNDLLRPALYGAIHSVESVSDGRTGRDRV
                     TADVVGPICETSDFFLRDSVMDL"
     gene            complement(1069..1545)
                     /locus_tag="VF958_01930"
     CDS             complement(1069..1545)
                     /locus_tag="VF958_01930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9147898.1"
                     /translation="MQPNPHFSDLLSEFNAARVRYLVVGAHVLSYYGRPRTTGDLDLW
                     VDPAPENAQRVFGAIARIGAPLEGITIEDFVRPGTVFQVGVAPSRIDVMTSLTGLNFT
                     NAWKGRRRARYGENPISVLSERDFIRNKRAVGRTRDLADAEEVELLLRRSRRPKRP"
     gene            complement(1719..2336)
                     /locus_tag="VF958_01935"
     CDS             complement(1719..2336)
                     /locus_tag="VF958_01935"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX9147899.1"
                     /translation="MFERVTAAARRIAPIRDVSFTVSADTTYAFVGREGSGKTAIVRC
                     ALGDIRPESGKVAVLGVDPHRERRALRRRVRFDETAGELRLDTDPPTALLFTSDPSRA
                     AAADRVGFLKEGHLVVEDALPLLLSRFRRIRYVNEVTETRTEYGTELDLFDAVRVKVR
                     GSSVDAVVSNYDDALFERFRATDGVRDARAEAMPLEEIFAAVVGR"
     gene            complement(2363..3694)
                     /locus_tag="VF958_01940"
     CDS             complement(2363..3694)
                     /locus_tag="VF958_01940"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HEX9147900.1"
                     /translation="MKIRRPAPPIRLMPTAPDSDSESNADAIARLRRDRVIYDGVTGF
                     PIHPFDDPERAHERIPHLGVIYLQIGKFFGFEELYGWELYDRVLVVVSRGIREDVEGS
                     RFAPHLFSIRYSGSDGFYVLYSLPAPGRGRGPSLENEATRLQGNAMKRLRTAFGGTPV
                     DLMTVHASSLLASDNPQVRSSRHVMRQLWETARIVSQRQTREKLDLCAQLKRVIGQKQ
                     LRPAFQPLRHLTDGAIIGWEALIRGPQGTPFERPNVLFAVAQENDMEVELETLCLETI
                     FRGLPRGIGSRRLFVNASSTLLCHPVFLDQRNLTAINRSHDDVVVEISEKEMVDYSSF
                     GDTLDIVRGAGLKIAIDDAGSGYSGLEAILKLRPDYIKVADSLVRRLESDPIKREIIS
                     SLASIGRRIDATLVAEGIEREEEREALIALGVEYGQGFLLGRPAFQISARA"
     gene            complement(3691..4077)
                     /locus_tag="VF958_01945"
     CDS             complement(3691..4077)
                     /locus_tag="VF958_01945"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX9147901.1"
                     /translation="MKRVVVVDDEPSVGVAVRDLLAPEGFEVDTPLDPQAALPELLRS
                     APDLVILDVNMPGMSGWELCSLLRRQTSTREVPVLFLTGRQEVRDRITAMQVGGSDYL
                     SKPFGADELRRKVRSLLKGRPTTEGR"
BASE COUNT          708 a         1388 c         1427 g          709 t
ORIGIN      
        1 ggagatccat gacgctgtct ctcaggaaga agtcgctcgt ctcgcaaatg gggccgacga
       61 catccgccgt cacgcggtct ctgccggtcc ggccgtccga aaccgattca accgaatgaa
      121 tcgcgccgta gagcgcgggg cggagaaggt cgttcatgcc ggcatccgta atcacgaaga
      181 gcctgcccga gttctctttc acgtagagga ccttcgtcaa gagagcgccg gccggtccga
      241 cgatcgcgcg accgggctcg atcaggatct tgaacgccag gtcgcggaag tgcggaagaa
      301 ccgctgccgc gtagcgttcc ggggtcagcg cctccgggcc ctcgccggca atgccgatac
      361 cgccgcccgc gtcgacggtc tcgatcggat gcccgtcgga agcgagcgcg accgcaagcc
      421 cggcgagatc gcgggcgctc tgagcgagcg gttccgggtc gaggatctgg ctcccgatgt
      481 gcgcctggac tcccgtcatg cggacgtgct cgagccggcg cgcgcgcgaa aagatctccc
      541 gggcccgtcc gatctccacg ccgaacttgt tgtgcctgcg gccggtcgag atgtaggggt
      601 gagagcgcgc gtcgatgtcg ggattgacgc gcagcgccac gcgcgcggtc acgccggcgg
      661 cagccgccgc ctcgatcttc tcgatctcgc gttccgactc ggcgttgatc gcgaggagct
      721 cgttctcgac ggcgaagcgg atctcctcgt ccgtctttcc gacgcccgag aacacgaccc
      781 ggtcagccgg gaagcccgag ccgagaaccg cacgcagctc gccggccgag acgacgtccg
      841 cgcccgcgcc gagctcggca agccggcgga gcagcgcgag gttcgaattc gccttggcgg
      901 cgtaacagac gaggtgccgc acgtcggcga aggcgtcgtc gaaccgacgg tagttctccg
      961 caatgagcgc gccgctgtag acgtaggccg gcgttccgac ctcccgcgcg atcgcggaca
     1021 gcgcgacccc gtcgcaggcg agctcgccgt cctcccggga gaagcccgtc aaggtcgctt
     1081 cgggcgtctc gagcgtcgaa gcaggagctc gacctcctcc gcatcggcga gatccctcgt
     1141 ccgcccgact gctctcttgt ttcgaatgaa gtcgcgttcc gagagcaccg agatcggatt
     1201 ctctccatag cgtgctctcc ggcgaccctt ccatgcgttc gtgaagttga gacccgtcag
     1261 ggacgtcatg acgtcgatcc gactgggtgc gacaccgacc tggaacaccg ttcccgggcg
     1321 aacgaagtcc tcgatcgtga ttccctcgag cggtgcgccg atgcgggcga tcgcaccgaa
     1381 aacacgctga gcgttttccg gcgccgggtc aacccacaga tcgaggtcgc cggtcgtcct
     1441 cggacgtccg taatagctaa gaacgtgtgc gccgacgacg aggtatcgga cccttgccgc
     1501 gttgaattcg gacaacagat cggagaagtg gggattcggt tgcattctgt ccggtgaggc
     1561 cataggcctc ttgcgaaagt ctccacgtct cggcaaaacg cgcctgtgct cctcttttgc
     1621 gccaataagc gacatcgcgg aggaagtcgg aatcctgaag cgtagtcagc cggctggtaa
     1681 ttttcttttt ttcaggcact tacgaaatta tatcgcggtc acctccccac gacggctgcg
     1741 aagatctcct cgagaggcat cgcctctgcg cgcgcatctc ggacgccgtc ggtcgcgcga
     1801 aagcgctcga aaagagcgtc gtcgtagttc gacacgaccg cgtcgacgct tgagccgcgg
     1861 accttcacgc gcacggcgtc gaagaggtcg agttcggtcc cgtactcggt gcgcgtctcg
     1921 gttacctcgt tgacgtaacg gatcctccga aaccgggaga gaagaagggg gagcgcgtcc
     1981 tcgacgacaa ggtgcccttc tttcaggaac ccgacccggt cggcggcggc ggcacggctt
     2041 gggtcgctgg tgaagagaag agcggtcggg ggatccgtat cgaggcggag ctcgccagcc
     2101 gtctcatcga accggacccg tcgacgcagg gcgcgacgct cccggtgcgg atcgacaccg
     2161 agaacggcga ccttcccgct ctccgggcgg atgtcgccga gggcgcaacg tacgatcgcg
     2221 gtcttcccgg agccttcgcg cccgacgaac gcgtaagtcg tgtctgcgga caccgtgaac
     2281 gagacgtcgc ggatcggggc gatccgccgc gctgcggccg tgacgcgctc gaacaagacg
     2341 gccggggggg cggctggcgt cgtcaggcgc gcgcggagat ctggaaggcc ggccgcccga
     2401 gcaaaaagcc ctgtccgtac tcgacgccga gcgcgatgag agcttctctc tcttcctcgc
     2461 gctcgatgcc ctcggcgacg agcgtcgcgt cgatgcgccg gccgatggaa gcgaggctcg
     2521 agatgatttc gcgcttgatg gggtcgctct cgagccgccg cacgagggag tcggcgacct
     2581 tgatgtagtc gggacgcagt ttgagaatcg cctcgagacc ggagtacccg gaccctgcgt
     2641 cgtcgatcgc gatcttcagg cccgcgccgc ggactatgtc cagcgtgtcg ccgaaggagg
     2701 agtagtccac catctccttc tccgagatct cgacgacgac gtcgtcgtgg ctgcggttga
     2761 tcgcggtcag gttcctctgg tcgagaaaga cggggtggca caggagggtc gacgacgcgt
     2821 tcacgaaaag tcgcctcgac ccgatcccgc ggggcagccc gcggaagatc gtctcgaggc
     2881 agagcgtctc gagctcgacc tccatgtcgt tctcctgcgc gaccgcgaag aggacgttcg
     2941 gacgttcgaa aggcgttcct tgcggtccgc ggatgagcgc ctcccagccg atgatcgctc
     3001 cgtcggtcag gtggcgcagc ggctgaaacg cggggcgcag ctgcttctgg ccgatcactc
     3061 gtttcaactg agcgcacagg tcgagcttct cgcgcgtctg gcgctggctc acgatccgcg
     3121 cggtctccca cagctggcgc atgacgtgcc gcgaggatcg gacctgaggg ttgtcgctcg
     3181 cgaggaggct cgacgcgtgg accgtcatga ggtcgaccgg tgttcctccg aaagccgtgc
     3241 gaaggcgctt catcgcgttg ccctgaagcc gggtcgcctc gttctcgagg gacgggccgc
     3301 ggccgcggcc gggagcgggc agcgagtaaa ggacgtaaaa gccgtcggag ccggagtagc
     3361 ggatcgagaa aaggtgtggc gcgaacctcg acccctcgac gtcctcacgg atcccgcgcg
     3421 agacgacgac gaggacgcgg tcgtagagct cccagccgta gagctcctcg aatccgaaga
     3481 attttccgat ctgcagatag atcactccca ggtgcgggat gcgctcatgg gcccgctcgg
     3541 gatcgtcgaa gggatggatc ggaaagccgg tgacgccgtc gtagatgacg cggtcccttc
     3601 gcaggcgagc gatggcgtcg gcgttcgact ccgaatcgga gtccggtgcc gtcggcatga
     3661 ggcggatcgg cggcgccggt cggcgtatct tcatcgtccc tccgtcgtcg gccggccctt
     3721 caggagcgac cgcaccttcc ggcgcagctc gtccgcaccg aagggtttcg agagatagtc
     3781 ggaccctccg acctgcatcg ccgtgatgcg gtccctcacc tcctgtcgtc cggtgagaaa
     3841 gagcacggga acctcgcggg tcgatgtctg acggcgcagc agcgagcaca gctcccatcc
     3901 gctcattccg ggcatgttca cgtcgaggat cacgagatcg ggcgcggagc gcagaagctc
     3961 cgggagcgcc gcctgggggt cgagcggggt gtccacctcg aagccttccg gcgcgagcag
     4021 gtcgcgcact gccacgccga ccgagggctc gtcgtcgacg acgacgacgc gtttcacgcc
     4081 gcgctccctt gcaaacgacc cgcgccgcat cggcacgcgg ctcggcaagt cggcctcgac
     4141 cgcgtgcctg gtcgttcgct gcggtcgcgc ttccgcggcg cgaggctcct ccctcgatta
     4201 cttcgggaga ctcctcgcgc cgcgacccgg ag
//
LOCUS       DASWAV010000090         2144 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U2665
            k141_524559, whole genome shotgun sequence.
ACCESSION   DASWAV010000090 DASWAV010000000
VERSION     DASWAV010000090.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960316
            Sequence Read Archive: SRR10260007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2144)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2144)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:47:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,929
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2144
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_524559"
                     /isolate="SMAG_U2665"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.36444 N 132.31889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1305
                     /locus_tag="VF958_01950"
     CDS             <1..1305
                     /locus_tag="VF958_01950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005032406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HEX9147902.1"
                     /translation="RCVARRPERLAGGGLEGVEVVRADVLDRESLPAALRDVEVAYYL
                     VHSLEAGPDFASRDFAGARNFAEAAREAGVRRIVYLGGLGAAERGPLSEHLRSRQETG
                     EALRSGGVPVVELRASVVLGAGSLSFEMIRALVERLPMMVWPRWVTVPTQPIGIEDVV
                     AYLLAALSLDIPTGESRIYEIGGPDRVSYGDLMREYARQRGLRRAFISVPVLTPKLSS
                     LWLRLVTPLQARVGRKLIEGVRNPTVVTDSSALAVFPIRPLGIRDAMARALAEETSRA
                     AKARRSFRTQILEREQDVPASAGEVFSFFSDPGNLARLTPPGMRFRIVAPGAGGLDAG
                     SRLEYRIRWTLLRLRWVTRITRWAPDSEFEDVQEEGPYRLWIHTHRFEPTARGVVMRD
                     RVEYELPFGPLGRLAHRLLVCRQLEAIFDFRRRAIEEIFPRR"
     gene            1302..>2144
                     /locus_tag="VF958_01955"
     CDS             1302..>2144
                     /locus_tag="VF958_01955"
                     /EC_number="4.1.99.3"
                     /inference="COORDINATES: protein motif:HMM:NF013071.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribodipyrimidine photo-lyase"
                     /protein_id="HEX9147903.1"
                     /translation="MTTIVHWFRRDLRVADNTSLSRAARDADRVVPVFVLDDHYLEGS
                     NVGPARFRFLRESLEELERALVAAGGCLVVRRGPPSRALPEILAETGASAVYANVEIG
                     PYPERRDRDVRAAVESAGGKLRLFGDALLVEPDAIATDAGDPYTVYSPFSRKWMAAEK
                     LPPLPAPRRLATLDLSSVPLARVRAWRDLPADFRAPRGGESEATRLLARFVEGPLARY
                     ERDRDVPAKDGTSRLSPHLHFGTISPRTVFAAVGEARADARKFQLELAWREFFHHMLF
                     HFPNV"
BASE COUNT          274 a          765 c          741 g          364 t
ORIGIN      
        1 cgttgcgtcg cgcgccgacc cgaacggctc gcgggcggcg gtctcgaagg agtggaggtc
       61 gtgcgggcgg acgtcctgga ccgcgagtcg cttccggccg ccctccgcga cgtcgaggtc
      121 gcctactacc tcgtccactc gctcgaggcc ggacccgact tcgcctcccg ggactttgcc
      181 ggggcgcgca atttcgcgga ggcggcgcgc gaggccggcg tccggaggat cgtctacctc
      241 ggaggtctcg gcgccgcaga gcgcggaccc ctctccgagc atctccggag tcgccaggag
      301 acgggagagg cgctccgatc cggcggcgtc cccgtcgtgg agcttcgcgc ttcggtggtg
      361 ctcggtgccg gaagcctgtc cttcgagatg atccgcgcgc tcgtcgaacg gctgccgatg
      421 atggtgtggc cgcgctgggt gacggtaccg acgcagccga tcggaatcga ggacgtcgtc
      481 gcgtacctcc tcgcggcact ctcgctcgac atccccaccg gcgagagccg gatctacgag
      541 atcggaggcc cggaccgcgt gtcgtacggc gatctcatgc gggagtacgc gcgtcagcgc
      601 gggctccgcc gcgcgttcat ttccgttccc gtcctcaccc cgaagctctc gagcctctgg
      661 cttcgcctcg tgactccgct ccaggcgcgc gtcggacgga aactgatcga gggggtgcgc
      721 aaccccacgg tcgtcacgga cagctcggcg ctcgcggtct ttccgatccg gcctctgggg
      781 attcgcgacg ccatggcgcg cgccctcgcc gaggagacat cccgcgccgc gaaagcccgg
      841 aggtcgttcc gcacgcagat tctcgagcgg gagcaggacg tcccggcatc tgccggcgag
      901 gtcttttcgt tcttctccga ccccgggaac ctcgcgcggc tcactccgcc gggcatgcgg
      961 ttccggatcg tcgctcccgg ggccggcggg ctcgacgcgg ggagccgtct cgaatatcgc
     1021 atccgctgga cgttgcttcg gctgcgctgg gtcacgcgga ttacccgatg ggcgccggac
     1081 tcggaattcg aggacgttca ggaggagggg ccataccggt tgtggattca cactcaccgt
     1141 ttcgagccca ccgcccgggg ggtcgtgatg cgcgaccgcg tggagtacga gcttcctttc
     1201 ggccccctgg gtcgtctcgc acaccgtctg ctcgtgtgcc gccagctcga agccatcttc
     1261 gacttccggc ggcgcgcgat cgaggagatc tttcctcgac ggtgacgacg atcgtccact
     1321 ggttccgccg cgacctgcgc gtcgcggaca acacgtcgct ctcgcgggcg gcgcgggacg
     1381 ccgaccgcgt cgtcccggtc ttcgtgctgg atgaccacta cctcgagggc tcgaacgtgg
     1441 ggccggcgcg atttcgcttt ctccgggaga gcctcgagga gctcgagagg gctctcgtcg
     1501 cggccggcgg ctgtctggtc gtccgacgcg gtcccccgtc gcgggcgctg ccggagatcc
     1561 tcgcggagac cggcgcctcc gccgtctacg cgaacgtcga gatcggtccg tacccggagc
     1621 ggcgcgaccg cgatgtgcgc gccgccgtcg agtcggccgg ggggaaactg cggctgttcg
     1681 gcgacgcgct gctcgtcgaa ccggacgcga tcgcgacgga cgccggagat ccgtacaccg
     1741 tgtactcccc gttctcgcga aagtggatgg ccgccgagaa gttacctccc cttccagcgc
     1801 cccggcgcct cgcgaccctg gatctgtcga gcgtccctct cgcgagggtg cgcgcgtggc
     1861 gcgacctgcc cgccgatttc cgtgcccccc gcggcggcga aagcgaggcg acgaggcttc
     1921 tcgcgcgttt cgtcgagggg ccgcttgccc ggtacgaacg ggaccgggac gtgccggcca
     1981 aggacggaac ctcccggctc tcgccgcacc tgcacttcgg gacgatctcg cctcgtacgg
     2041 tcttcgcggc ggtcggggaa gcgcgtgccg acgcccgaaa gttccagctc gagctcgcct
     2101 ggcgcgagtt ctttcaccac atgctgtttc acttccccaa cgtc
//
LOCUS       DASWAV010000091         2987 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U2665
            k141_524760, whole genome shotgun sequence.
ACCESSION   DASWAV010000091 DASWAV010000000
VERSION     DASWAV010000091.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960316
            Sequence Read Archive: SRR10260007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2987)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2987)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:47:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,929
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2987
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_524760"
                     /isolate="SMAG_U2665"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.36444 N 132.31889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..817
                     /locus_tag="VF958_01960"
     CDS             <1..817
                     /locus_tag="VF958_01960"
                     /inference="COORDINATES: protein motif:HMM:TIGR04312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="choice-of-anchor B family protein"
                     /protein_id="HEX9147904.1"
                     /translation="TGYAGYGYVHQGWLTEDQSYFLVDDELDETQFLHNTRTYVFDVR
                     LLDAPVLVGYYTGPSPAIDHNLFVRGGYAYEANYTSGLRILDLAGVAAATLSEAGYFD
                     VVPENDDRVYSGAWGNYPFFASGIVLVSGIEQGLFVLKPRLPTPPGASFYSVTPCRLL
                     DTRAPSGIAGGPALTAGTARLVSVAGRCGIPPGAREAAVNVTITGASAPGHLTLTSPE
                     SISPRTSTINYRPGETRANNAIVALESGGELRVFCTQPSGSVQMILDVSGYFR"
     gene            complement(821..1681)
                     /locus_tag="VF958_01965"
     CDS             complement(821..1681)
                     /locus_tag="VF958_01965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008446856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HEX9147905.1"
                     /translation="MRTLIHVSDLHFGRINPATLGPLLDSARQLSPDLVVVSGDLTQR
                     ARASQFREARDFLIALPKPQIVVPGNHDVPLYDVVSRFLRPLANYRRYVTDDLEPFYR
                     DEEIAAIGINTARSLTFKGGRINEFQVARVREKLCALTPETTRIIVTHHPFDLPEGRA
                     SADLVGRARMAMETFVACGCDVILSGHLHVSHTGHTAIRYRLPGRSALLVQAGTATST
                     REREEPNSFNVLRIDPPRIEVEIRVFENDSRRFVSAATEVFERVGDSWERVMPRERPE
                     DEPAGARPAG"
     gene            complement(1678..2610)
                     /locus_tag="VF958_01970"
     CDS             complement(1678..2610)
                     /locus_tag="VF958_01970"
                     /inference="COORDINATES: protein motif:HMM:NF039905.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HEX9147906.1"
                     /translation="MDVGKDMRKLLPVLLNRTSGDSDARDVPDRIREAFRSAGADADV
                     RIAGDGEQFGALAREAAAGDSDAIVAAGGDGSVGSVAAVLAGSGKPLGVLPLGTLNHF
                     AKDLGVPLDLDGAVRTVVEGRVARIDVGEVNGRIFVNNSSLGLYPRIVHRREQKREKL
                     GWGKWPAFVWATLHALHRHRSVDVVLVADGQEIRRRTPFVFVGNNFYEMAGFDIGTRE
                     RLDRGELSAYLAPGAKPVDFLLFGLRALIGRLRGTPGFEVLRATELRIETRGDRARVA
                     TDGEISIVSTPLRYRLLPGALSVIVPRETLEEAP"
     gene            2670..>2987
                     /locus_tag="VF958_01975"
     CDS             2670..>2987
                     /locus_tag="VF958_01975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9147907.1"
                     /translation="MDFSRRNAAGRALAIASFFAFCALGGGVPLLAAGVSIPGAYSDS
                     ARALATAPVRLSDLANAAERLTPGERHALPPLSDDERRELARREKGLAKVGLFRRLES
                     PVRL"
BASE COUNT          471 a         1029 c          997 g          490 t
ORIGIN      
        1 caccggatat gccgggtacg ggtacgtgca ccagggatgg ctcaccgagg accagtcgta
       61 cttcctcgtc gacgacgagc tcgacgaaac ccagttcctc cacaacaccc gtacctacgt
      121 cttcgacgtt cgtctgctcg acgcgccggt tctcgtcggc tattacacgg gtccgtctcc
      181 cgccatcgac cacaacctgt tcgtgcgagg cggctacgcg tacgaggcga actacacgag
      241 cggtctccgg attctcgacc tcgccggcgt cgcggcggcc acgctgtcgg aggcgggtta
      301 cttcgacgtc gtgccggaaa acgacgaccg ggtttacagc ggagcctggg gaaactatcc
      361 gttcttcgcg agcggaatcg ttctcgtctc ggggatcgag cagggccttt tcgtgctgaa
      421 gccccgcctg ccgacgccgc ccggcgcctc gttctacagc gtgacgccct gccgcctcct
      481 ggacacgagg gcgccgtcgg gcatcgccgg gggacccgcg ctgaccgcgg ggacggcgag
      541 gctggtgtcg gtcgccggcc ggtgcggcat tccccccgga gcgcgagagg ccgcagtcaa
      601 cgtcaccatc acgggagctt cggcgcccgg gcatctcact ctgacctcac ccgagtcgat
      661 ctctccccgc acgtcgacga tcaactaccg tccgggagag acgcgcgcga acaacgccat
      721 cgtcgcgctc gagtcgggtg gagagctccg cgtgttctgc acgcagccgt ccggctccgt
      781 ccagatgatc ctggacgtct ccggatactt tcgctgaccg tcagcccgcg ggccgcgcgc
      841 ccgccggctc gtcttcggga cgctcgcggg gcatcacgcg ctcccagctg tcgccgaccc
      901 tttcgaagac ttccgtcgcc gcggagacga accgccgtga atcgttttcg aagaccctaa
      961 tctcgacctc gattcggggc gggtcgatcc ggagcacgtt gaaggagttc ggctcctcgc
     1021 gctcgcgggt ggacgtcgcc gtgcccgcct gaacgaggag cgccgatcga ccgggaaggc
     1081 ggtaacggat cgcggtgtga ccggtgtgag agacgtgcag gtgtccggag aggatcacgt
     1141 cgcaaccgca ggcgacgaac gtctccatcg ccatgcgcgc ccgcccgacg aggtcggcac
     1201 tcgcgcgccc ttcgggcagg tcgaacggat ggtgcgtgac gatgattcgc gtcgtttcgg
     1261 gcgtcagcgc gcaaagcttc tcgcggactc gggccacctg gaactcgttg atccggcccc
     1321 ccttgaacgt gagcgatcgg gccgtgttga tcccgatcgc cgcgatctcc tcgtcccggt
     1381 agaacggctc gagatcatcg gtcacgtagc ggcgatagtt tgcgagcggc cgcaggaacc
     1441 gggagacgac gtcgtagagc gggacgtcgt ggttgcccgg gacgacgatc tgcggcttcg
     1501 gcagagcgat gagaaagtcc cgcgcctccc ggaactgcga cgccctcgcc cgctgcgtga
     1561 ggtcgcccga cacgacgacg aggtcgggcg agagctgacg cgcgctgtcg agaagcggtc
     1621 cgagcgtcgc agggttgatg cgcccgaagt ggagatcgga aacgtggatc agcgtcctca
     1681 cggggcctct tcgagtgtct cgcgggggac gatcaccgag agagctcccg gcaacaggcg
     1741 ataccggagc ggcgtcgaaa cgatggagat ctcgccgtcg gtcgcgacgc gggcgcggtc
     1801 gccgcgagtc tcgattcgca gctccgtcgc tcgcagcacc tcgaacccgg gagtgccgcg
     1861 caaccgaccg atcagggctc gcagcccgaa caagaggaaa tcgaccggtt tcgcgccggg
     1921 agcgaggtag gcggagagtt ctccgcgatc gagacgctct cgcgtgccga tgtcgaatcc
     1981 cgccatctcg tagaagttgt tcccaacgaa aacgaacggc gtccgccgcc ggatctcctg
     2041 gccgtcggcg acgaggacga cgtcgacaga gcgatgccgg tgaagggcgt gaagggtcgc
     2101 ccagacgaac gccggccact ttccccagcc gagcttctcg cgtttctgct cgcggcggtg
     2161 gacgatccgg ggatacagcc cgaggctcga gttgttgacg aaaatccggc cgttgacctc
     2221 gccgacgtcg atcctcgcga cccggccttc cacgacggtc cggaccgctc cgtcgagatc
     2281 gagcggtacc cccagatcct tggcgaagtg gttgagggtg ccgagcggca acacgccgag
     2341 cggcttgccg cttcctgcga gcacggcggc gaccgaaccg acgctgccgt cgccgccggc
     2401 ggcaacgatg gcgtcggaat cgccggctgc cgcctcccgc gcgagcgcgc cgaactgctc
     2461 accgtctccc gcaatccgca cgtccgcatc ggcgcccgcc gagcggaacg cttcccgaat
     2521 tcggtcaggg acgtcgcggg cgtcgctgtc gcccgaagtt cgattgagca gtaccggcag
     2581 aagcttgcgc atgtccttcc cgacgtccac ggcgggaagt gcaagagtcc ggcccgtgcc
     2641 gtatgctccc gggcaaaagg ggatagatca tggacttttc gcgccgaaac gccgcggggc
     2701 gcgctcttgc catcgcctca ttcttcgctt tttgtgctct cggtggcggg gttccgctcc
     2761 tggccgccgg cgtctcgatt ccgggtgcct actccgattc ggcgcgggcg ctcgccaccg
     2821 ccccggtccg cctctcggac cttgcgaatg cggcggagcg cctgacgccc ggcgagcgac
     2881 acgcgctccc tcctttgtcc gacgacgagc ggcgcgaact cgcccgccgg gagaaagggc
     2941 tcgcgaaggt cggtctgttc cgccgcctcg aatctccggt ccgcctc
//
LOCUS       DASWAV010000092         1586 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U2665
            k141_85131, whole genome shotgun sequence.
ACCESSION   DASWAV010000092 DASWAV010000000
VERSION     DASWAV010000092.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960316
            Sequence Read Archive: SRR10260007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 1586)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1586)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:47:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,929
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1586
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_85131"
                     /isolate="SMAG_U2665"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.36444 N 132.31889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..753)
                     /locus_tag="VF958_01980"
     CDS             complement(<1..753)
                     /locus_tag="VF958_01980"
                     /inference="COORDINATES: protein motif:HMM:NF013702.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HEX9147908.1"
                     /translation="MPTTVAFPLWLAIIVIALAAWAFVGHLLLPGWRWILRRRVNRVL
                     EEIDTRLKIRIPPFKLTKREVLISRVIDDRELQEAIEAEASATGVPRSAVQRRVEGWA
                     REIVPEFNAYLYFRIGYALARWIGRSLYRLRLGYSDEEGLAALDPSATVVFLINHRSN
                     MDYVLVSYLAAEKAALSYAVGEWARIWPLQTLIRSMGAYFVRRNSKDPLYRKVLERYV
                     AMATAAGVTQAVFPEGGLSREGSLRAPKLGILD"
     gene            complement(856..1437)
                     /locus_tag="VF958_01985"
     CDS             complement(856..1437)
                     /locus_tag="VF958_01985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015490198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HEX9147909.1"
                     /translation="MAAVALPTPASSETAKTYVIDKTHSDVSFQVRHLVTKVRGRFAD
                     FDAEIRAVPEAPEESSVRFAIRTASVDTDLPDRDQHLRSADFFDADEHPEITFVSSRI
                     RSAGDDRYDVTGLLTLRGVAREMTLPVSFLGFVSDPWGNEKAGFETEVVLNRKDFGMV
                     WNAALDNGGVVLGDKVTVSISLEAVRARDAAAA"
BASE COUNT          285 a          503 c          530 g          268 t
ORIGIN      
        1 gtcgaggatg ccgagcttcg gcgctcggag gctcccctcg cgcgagagtc ccccctccgg
       61 gaagaccgcc tgtgtgacgc ccgcggccgt cgccatcgcg acgtaacgct cgagcacctt
      121 ccggtagagc gggtccttcg aattgcgccg gacgaaatag gcgcccatcg aacggatcaa
      181 cgtctggagc ggccagattc gcgcccactc gcccaccgcg taggagaggg cggccttctc
      241 cgccgcgaga taggagacga ggacgtagtc catgttgctg cggtgattga tcaagaacac
      301 gaccgtcgct gagggatcga gcgccgcgag cccctcctcg tccgagtagc cgagccgcag
      361 gcggtagaga gaacgaccga tccagcgcgc cagcgcgtat ccgattcgaa agtagagata
      421 cgcgttgaac tccgggacga tctcgcgcgc ccagccctcg acgcgtcgct ggaccgcgga
      481 acggggaacg ccggtcgcac tcgcctcggc ctcgatcgcc tcctggagct cccgatcgtc
      541 tatcaccctg gaaatcagga cctcgcgctt cgtcagcttg aacggcggga tgcggatctt
      601 caggcgggta tcgatttcct cgagaacgcg gttcacgcgt cgtcgaagga tccatcgcca
      661 gccggggaga aggagatggc cgacgaacgc ccacgccgcg agtgcgatca cgatgatcgc
      721 cagccacagc ggaaaagcaa cggtcgtcgg catgatgggg ggagccggtc gcacgaccgg
      781 tgggccgaat cttatccgct ccgaggcggg gaagcggagg cgcgccgggt tccaaccgcc
      841 gcgcctcgcg gtgttttacg cggccgcggc gtccctggcc cggaccgcct cgagagagat
      901 cgagacggtg accttgtcgc cgaggaccac gccgccgttg tcgagagcgg cattccagac
      961 catcccgaag tccttgcggt tcagaacgac ctcggtttcg aagccggcct tctcgtttcc
     1021 ccaggggtcc gagacgaagc cgaggaagct cacgggaagc gtcatctctc tcgcgactcc
     1081 ccggagcgtg agaagtccgg tgacgtcgta ccgatcgtcg ccggccgatc ggatccggct
     1141 gctgacgaag gtgatctccg gatgctcgtc ggcgtcgaaa aagtcggccg agcgcaggtg
     1201 ctgatcccgg tcgggcaggt cggtgtccac ggaagcggtt cggatcgcga atcgcaccga
     1261 ggactcctcg ggcgcctcgg gaactgcccg gatctcggcg tcgaagtcgg cgaaccgacc
     1321 gcgcaccttc gtcacgaggt gacggacctg gaacgagacg tcggaatgcg tcttgtcgat
     1381 cacgtaggtc tttgcggttt cgctggaagc gggggtgggg agggcgacgg cggccatggg
     1441 ggatctcctt ccttgatctc taaatagtta actaatcatc taatctcgac gtctgaatta
     1501 cgcatgccgg cggcggagag tttccagggc tcggttcacc cgccgaacgg tcgtctttcc
     1561 aagggtttgt gcgaaagcct cctcgg
//
LOCUS       DASWAV010000093         2166 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U2665
            k141_645027, whole genome shotgun sequence.
ACCESSION   DASWAV010000093 DASWAV010000000
VERSION     DASWAV010000093.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960316
            Sequence Read Archive: SRR10260007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2166)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2166)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:47:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,929
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2166
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_645027"
                     /isolate="SMAG_U2665"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.36444 N 132.31889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1975
                     /gene="fusA"
                     /locus_tag="VF958_01990"
     CDS             <1..1975
                     /gene="fusA"
                     /locus_tag="VF958_01990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006716333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HEX9147910.1"
                     /translation="MDWMVQEQERGITITSAATTCFWQAGSASGDSSEGSGPLVRINI
                     IDTPGHVDFTAEVERSLRVLDGAIAVFDAVAGVEPQSETVWRQANRYGVPRLAFVNKM
                     DRVGANFPRCVDMMRTKLAAKPAAIQIPWGSEAEHRGVIDLVEMKGIDFQDDTLGSKF
                     EVTGIPAELREEADVYREKLIETIAETDDALLEKYLHGGKVEVDELKAALRRATIAGD
                     VQPVVCGSAFKNKGVQQLLDAVVDYLPSPLDVPPIVGHDEVGAEMIREPKDDAPFSAL
                     VFKIMTDPFVGQLAFFRVYSGHLDAGSAVYNSTRDKSERIGRILKMHANKREEIKEVW
                     AGDIAAAVGLRHVSTGDTICDEKAPVILEAMNFPEPVIAVAIEPKTKADQEKMGLALS
                     KLMQEDPTFKVHTDRETGQTIIRGMGELHLEIITDRLVREFSVGANIGKPQVAYREAI
                     TREAEGHGRFVRQSGGRGQYGDCKIRLKPAEPGHDFVFDNQIVGGKIPKEFIKPIEHG
                     IKEAMETGVLAGYPTTGVEVELYDGSYHDVDSSEMAFKIAGSMAYQDAARKAKPVLKE
                     PIMDVEAVVPEEYMGDVIGDLNSRRGHITHMEPRAGSQIIAARVPLSEMFGYSTNLRS
                     MTQGRGTYTMQFAAYDEVPKSVSEEIIAKVGSK"
     gene            1991..>2166
                     /locus_tag="VF958_01995"
     CDS             1991..>2166
                     /locus_tag="VF958_01995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005583512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HEX9147911.1"
                     /translation="MGKEKFDRSKPHVNIGTIGHVDHGKTTLTSAITLVLAKKGLAQA
                     KAYDQIDNAPEEKAR"
BASE COUNT          439 a          664 c          731 g          332 t
ORIGIN      
        1 gatggattgg atggtccagg agcaggagcg cgggatcacg atcacgtccg ccgcgacaac
       61 gtgcttctgg caggcgggct ccgcatccgg cgactcttcc gagggttcgg gcccgctcgt
      121 ccggatcaac atcatcgaca cccccggcca cgtcgatttc accgccgagg tcgagcgttc
      181 gctgcgggtc ctcgatgggg cgatcgccgt cttcgacgcg gtcgccgggg tcgagccgca
      241 gtccgagacg gtgtggcgtc aggcgaaccg gtacggagtc ccgcggctcg cgttcgtcaa
      301 caagatggac cgtgtgggcg cgaacttccc ccgctgcgtc gacatgatgc gcacgaagct
      361 cgccgcgaag cccgcagcca tccagatccc gtgggggagc gaggccgagc accgcggggt
      421 gatcgatctc gtcgagatga aggggatcga ctttcaggac gacactctcg gctcgaagtt
      481 cgaggttacg ggcatccccg ccgagcttcg cgaagaggcc gacgtctacc gagagaagct
      541 gatcgagacg attgccgaga ccgatgacgc tctgctcgag aagtacctgc acggcggaaa
      601 ggtcgaggtc gatgagctca aggcagcgct gcggcgggcg acgatcgccg gcgacgtgca
      661 gccggtcgtc tgcgggtccg ctttcaagaa caagggggtc cagcaactcc tggacgccgt
      721 cgtggactac ctgccgtcgc cgctcgacgt ccctccgatc gtcggacacg acgaggtcgg
      781 ggccgagatg attcgcgagc cgaaggacga cgcgccattt tccgcgctcg tcttcaagat
      841 catgaccgat cccttcgtcg gtcagctcgc cttcttccgc gtctattcgg gccacctcga
      901 tgcgggttcc gccgtctaca actcgacccg cgacaagagc gagcggatcg gccgcatcct
      961 gaagatgcac gcgaacaagc gcgaggagat caaggaagtc tgggcgggtg acatcgccgc
     1021 cgccgtcggc ctgcggcacg tctcgacggg cgacacgatc tgcgacgaga aagccccggt
     1081 cattctcgag gccatgaact ttcccgagcc cgtcatcgcg gtcgcgatcg agcccaagac
     1141 gaaggccgac caggaaaaga tgggtctcgc cctctcgaaa ctgatgcagg aggacccgac
     1201 gttcaaggtc cacacggacc gcgagacggg acagacgatc atccgcggaa tgggggaact
     1261 gcatctcgag atcatcacgg accggttggt ccgggagttc agcgtcggcg ccaacatcgg
     1321 caagccgcag gtcgcgtacc gcgaggccat cacccgagag gcggaggggc acggacgttt
     1381 cgtccgccag tcgggggggc gcgggcagta cggcgactgc aagatccgct tgaagccggc
     1441 ggagcccgga cacgacttcg tcttcgacaa ccagatcgtc ggaggcaaga tcccgaagga
     1501 gttcatcaag ccgatcgagc acgggatcaa ggaagcaatg gagacgggcg tgcttgcggg
     1561 atatccgacg accggggtgg aggtcgagct ctacgacggc tcctaccacg acgtcgattc
     1621 ttccgagatg gctttcaaga tcgcgggatc catggcgtac caggacgccg cgcgcaaggc
     1681 gaagccggtc ctgaaagagc cgatcatgga cgtcgaggcc gtcgttcccg aggagtacat
     1741 gggcgacgtg atcggagacc tcaactcacg ccgcggccac atcactcaca tggagccgcg
     1801 cgcgggctcg cagatcattg ccgcccgcgt gcccctgtcg gagatgttcg ggtattccac
     1861 gaacctccgc tcgatgaccc agggccgcgg gacctacacg atgcagttcg cggcctacga
     1921 tgaagtgccg aagtccgttt ccgaagagat catcgccaag gtgggaagca agtaaccgcg
     1981 agggagaagg atgggcaagg aaaagttcga tcgcagcaag ccgcacgtca acattgggac
     2041 gatagggcac gtggatcacg ggaagacgac gttgacctct gcgatcacgc tggtgttggc
     2101 gaagaagggg ttggcgcagg ccaaggcgta cgaccagatc gacaacgcgc cggaggagaa
     2161 ggcgcg
//
LOCUS       DASWAV010000094         5138 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U2665
            k141_404526, whole genome shotgun sequence.
ACCESSION   DASWAV010000094 DASWAV010000000
VERSION     DASWAV010000094.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960316
            Sequence Read Archive: SRR10260007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 5138)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5138)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:47:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,929
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5138
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_404526"
                     /isolate="SMAG_U2665"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.36444 N 132.31889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..354
                     /gene="acpS"
                     /locus_tag="VF958_02000"
     CDS             <1..354
                     /gene="acpS"
                     /locus_tag="VF958_02000"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011688356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HEX9147912.1"
                     /translation="EVDRVAAVLDRWGDRFVERVFRPGELSRVRRHPRARAEHVAGRF
                     AAKEAAMKALGTGWHGVAFRDIEVGRDARGKPVLALHGRALSRAHELGVASMEVSITH
                     GSRTAAAVVAFLVED"
     gene            395..3328
                     /gene="ileS"
                     /locus_tag="VF958_02005"
     CDS             395..3328
                     /gene="ileS"
                     /locus_tag="VF958_02005"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR00392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004822 - isoleucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006428 - isoleucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HEX9147913.1"
                     /translation="MADTPDYKATLNLPKTAFPMKADLSTREPERLAAWKRMDLESAI
                     RAASAGRPRFILHDGPPYANGNIHIGHALNKVLKDFIVRSRTMLGFDAPYVPGWDCHG
                     LPIERKVDKELGPKKREMSAVEIRRACRDYAQRFVDIQRGEFERLGVGGLWERPYTTM
                     SPAYEAEIARAFGQFYEKGLVYQALKSVRWCFSDRTALAEAELEYGERIDPAIDVAFE
                     LEKGAASRFGVSSEARVFALIWTTTPWTIPSNVAIAVHPDETYQLMEEGDRLYVVAEK
                     LAPDVARRAGWRSPRLAGTARGTGLVGLRYRHPLPPEMRGELTAEEVARSFRLVAGDH
                     VTMDAGTGLVHTAPGHGEDDFQTGVRENLPTLSPVDEAGRFTAVARYRGEKVLDANPK
                     IVKDLEEAGALAAVDEKFRHEYPHCWRCKNPVIFRATVQWFIRLDDPALDLRKGALDA
                     IGRVQWVPPWGEARIGGMVENRREWVISRQRRWGSPITLLYARRDGNQEVYPWRDSPA
                     AQRKFFERVVSIFRREGADAWYARPATDFLPDDADLRGYSKADFQAETDILDVWFDSG
                     VSHLAVLRHGEWPELRRPEGEIPADLYVEGHDQHRGWFQSSLLTSVALYGDAPYRGVV
                     THGFVVDVSSGKKMSKSVGNVIEPRELIPRYGADVIRLWVASADYRDDDPISEEILAR
                     CAEAYRKIRNTARYLISNLYDFDPGVNEVGPADLLPIDRWALGETKRLSARVSDAYRG
                     YEFHLVYHSLVNFCATTLSAFYLDVLKDRLYASPAGSRERRSAQTAMSRIGRCIATLA
                     APVMPFTAEEIWQELPGQKEESVHLARFEARDDLPKEPLPEAAWDRVMKLREEAAVLL
                     EAARKEKTIGSSLEGAIAFTPSADLDRDRHAIGAEGAGLADLFIVSETIPAAADAEGA
                     GWTTSQAYPNMRIRFEKARGRRCDRCWKVTPEANDDGLCDRCRRALATLATAGASTPC
                     APFPA"
     gene            3307..3837
                     /gene="lspA"
                     /locus_tag="VF958_02010"
     CDS             3307..3837
                     /gene="lspA"
                     /locus_tag="VF958_02010"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015838958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HEX9147914.1"
                     /translation="MRALPRVKESALYRAGYFLVSLAVLLLDQWTKGIVMRTFQIHES
                     REVVSGFFDLTYVQNSGAAFGLFASVDSSVKAIVLNSVAVLVFLIVSAYAIRSSHKSV
                     RLQVGLALILGGAVGNLLDRVRFTYVVDFLDLSISGHHWPAFNLADSAICIGVGLLFL
                     DMLQKEEEAEAAPPVT"
     gene            3843..4637
                     /gene="lgt"
                     /locus_tag="VF958_02015"
     CDS             3843..4637
                     /gene="lgt"
                     /locus_tag="VF958_02015"
                     /EC_number="2.5.1.145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005009815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HEX9147915.1"
                     /translation="MFPRLIQIGSFYIPTYGVILAIAYLVGIWLLRRKARAEGLPDQN
                     IFDFSLYILAAAILGAKLLLVIVEWRRYAENPRDLVEVLRSGGVFYGGLIAATAVGIW
                     YMKKHRLPAWKIADMGAPSIALGEAIGRWGCFAAGCCYGRPTDSPLGVTFTDPFAHDA
                     VGTPLNVPLHPTQIYLSLNALLIFLILQWVYRRRTFDGEVFWLYVLLYAITRGILETF
                     RGDSIRGFLIPGVLSTSQFIGVLAAVAAAGMLVILSRRRRAAASSS"
     gene            4634..>5138
                     /locus_tag="VF958_02020"
     CDS             4634..>5138
                     /locus_tag="VF958_02020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012302048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HEX9147916.1"
                     /translation="MIRRFRVGTPSEGTRLDLFLASACSDLSRSRIQKLIAEGAVTLD
                     GVPAKRSHVVRAGGRVEVDVPEPRAAAIEPEEIPLSILYEDEHLLAIDKPSGLVVHPS
                     PGHDSGTLVNALLHHVRDLSGIGGELRPGIVHRLDRDTSGVLLVAKSDRAHASLSRQM
                     KNRALKKE"
BASE COUNT          793 a         1791 c         1673 g          881 t
ORIGIN      
        1 gaagtcgatc gcgtcgcggc agttctcgac cggtggggcg atcgcttcgt ggagcgcgtc
       61 ttccgcccgg gagaactctc ccgggtccgg cgtcacccgc gggcccgcgc cgagcacgtt
      121 gccggccgct tcgcggcgaa ggaggcggcc atgaaggcgc tgggtacggg ctggcacgga
      181 gtcgctttcc gcgacatcga agtcggccgg gacgcccgcg gaaagcctgt cctcgcgttg
      241 catggccggg ccctctcccg cgcgcacgag ctcggcgtcg cgagcatgga ggtctcgatc
      301 acgcacggct cgagaaccgc tgcggcggtc gtcgcgttcc tcgtcgagga ttgatcggcg
      361 cccgcgaaac gcttatcgtt ccgcctcctc gatcatggcc gacactcccg actacaaagc
      421 gacattgaac ctgccgaaaa ccgccttccc gatgaaggcg gacctttcca ctcgggaacc
      481 ggaacgactc gcggcctgga aacgcatgga cctcgagagc gcgatccgcg ctgcgtcggc
      541 ggggaggccc cgtttcatcc tccacgacgg tccgccttac gccaacggca acatccacat
      601 cggacacgct ttgaacaaag tcctcaagga cttcatcgtg cgcagccgca cgatgctcgg
      661 cttcgacgcg ccgtacgttc cgggctggga ctgccacggc ctcccgatcg agcggaaggt
      721 ggacaaggag ctcgggccga agaagcgcga aatgtcggcc gtcgagatcc gccgcgcgtg
      781 ccgcgattac gcccagcgct tcgtcgacat ccagcgcgga gagttcgagc ggctgggcgt
      841 cggaggtctc tgggagcgcc cgtacacgac gatgagcccc gcctacgagg cggagatcgc
      901 ccgcgcgttc gggcagttct atgagaaggg cctcgtctac caggcgctca agtcggtccg
      961 ctggtgtttc tccgaccgga cggcgctcgc cgaggcggag ctcgagtacg gcgagcgcat
     1021 cgatccggcg atcgatgtgg cgttcgaact cgagaagggc gcggcatccc gtttcggcgt
     1081 ttcctccgaa gcgcgcgtct tcgcgctgat ctggacgacg actccgtgga cgatcccctc
     1141 caacgtcgcg atcgcggtcc accccgacga gacgtatcag ctgatggagg agggagaccg
     1201 gctctacgtc gtcgccgaga agctcgcgcc ggacgtggcg cgacgcgccg gctggcgatc
     1261 gccgagactc gctggaacgg cccggggcac cggtctcgtg gggctgcgat accggcaccc
     1321 gctccctccg gagatgcgcg gtgagctcac tgccgaggag gtcgcgcgat ccttccgcct
     1381 cgtcgccggc gaccacgtga cgatggacgc ggggaccggc ctcgtgcaca cggcgcccgg
     1441 ccacggcgag gacgacttcc agaccggagt ccgcgagaac ctgcccaccc tctcccccgt
     1501 cgacgaggcc ggccgcttca cggcggtcgc gcgctaccgc ggcgaaaagg tgctcgacgc
     1561 gaacccgaag atcgtgaagg acctggagga agccggtgcc ctcgccgcgg tcgacgagaa
     1621 gttccgccac gagtacccgc actgctggcg ctgcaagaac ccggtcatct tccgtgcgac
     1681 ggtgcagtgg ttcatccgcc tcgacgaccc cgctctcgat ctgcggaagg gggcgctgga
     1741 cgcgatcgga cgcgtgcagt gggtcccgcc gtggggggaa gcgcggatcg gtgggatggt
     1801 cgagaaccgg cgcgagtggg tgatttcccg ccagaggcgc tggggctcgc cgattacgct
     1861 cctctacgcg agacgcgacg ggaaccagga ggtctacccg tggagagact cgcccgccgc
     1921 gcaacggaaa ttcttcgaga gggtcgtttc gatcttccgg cgcgaaggcg ccgacgcgtg
     1981 gtacgcccga ccggcgaccg acttccttcc cgacgacgcg gatctgcgcg gctactcgaa
     2041 ggccgacttc caggcggaga ccgacatcct cgacgtctgg ttcgattccg gcgtttcgca
     2101 cctggcggtc ctgcggcacg gcgagtggcc ggagctccgg cgaccggagg gtgagattcc
     2161 ggcggatctt tacgtcgagg ggcacgacca gcaccgcggc tggttccagt cgtccctgct
     2221 cacgtccgtc gccctctacg gcgatgcgcc ctatcgtggg gtcgtgaccc acggcttcgt
     2281 cgtcgacgtg tcgtcgggca agaagatgtc gaagtcggtc ggaaacgtca tcgagccgcg
     2341 cgagctcatt cccagatacg gggcggacgt gatccgcctc tgggtggcga gcgcggacta
     2401 ccgcgacgat gatccgatct ccgaggagat cctcgcgcgc tgcgccgagg cgtaccggaa
     2461 gatccgcaac acggctcggt atctgatctc gaacctctac gacttcgatc cgggcgtgaa
     2521 tgaagtcggt cccgccgatc tcctcccgat cgatcgctgg gcgctcgggg agacgaagcg
     2581 gctgtccgcg cgcgtttccg acgcttaccg cggttacgag ttccacctcg tgtaccactc
     2641 gctcgtcaac ttctgcgcga cgaccctctc cgcgttctac ctcgacgtgc tgaaggaccg
     2701 gctgtatgcg tcgcccgccg gatcccggga gcggcgctcg gcccagacgg cgatgtcccg
     2761 gatcgggcgg tgtattgcga cgctggccgc tcccgtcatg ccctttacgg ccgaggagat
     2821 ctggcaggag ctcccgggtc agaaggaaga atccgtccac ctcgcgcgtt tcgaggcccg
     2881 ggatgacctt ccgaaagagc ccctgcccga agcggcctgg gatcgtgtga tgaaactgcg
     2941 cgaagaggcc gccgtcctcc tcgaagcggc gcgtaaggag aagacgatcg gttcctccct
     3001 ggagggggcg atcgccttca cgccgagcgc ggacctcgat cgcgatcggc acgcgatcgg
     3061 tgcggaaggc gcgggtctcg ccgacctgtt catcgtgtcc gagacgatcc cggcggccgc
     3121 cgacgccgag ggcgcgggct ggaccacgtc ccaggcctac ccgaacatga gaatccggtt
     3181 cgagaaagcg agggggcgcc gctgcgaccg ctgctggaag gtcacccccg aggcgaacga
     3241 cgacggcctc tgcgatcgtt gtcgccgggc cctcgcgacc ctcgccaccg cgggagcatc
     3301 caccccgtgc gcgcccttcc ccgcgtaaag gagagcgctc tctatcgcgc cggctatttc
     3361 ctcgtgtcgc tcgccgtcct cctgctcgat cagtggacga agggcatcgt gatgcgcacg
     3421 ttccagattc atgaatcgcg cgaggtcgtc tccggcttct tcgacctcac ctacgtgcag
     3481 aacagcggcg ctgcgttcgg cctttttgcg tcggtggact cttccgtgaa agcgatcgtg
     3541 ctgaacagcg tcgcggtcct cgtgttcctc atcgtctcgg cctacgcgat tcgctcctcg
     3601 cacaagagcg tccggctcca ggtcggcctg gcgctcatcc tcggcggcgc cgtgggcaat
     3661 cttctcgacc gggtccgctt cacgtacgtg gtcgacttcc tcgacctctc catctcgggg
     3721 caccactggc ccgcgttcaa cctcgccgac tcggcgatct gcatcggagt cgggctcctc
     3781 ttcctcgaca tgctccagaa agaggaggag gccgaggccg cgcctcccgt cacgtaagcg
     3841 agatgttccc ccgcctgatc cagatcggct cgttctacat cccgacctac ggcgtcatcc
     3901 tcgcgatcgc ctatctcgtc gggatctggc tgcttcgccg caaggcccgc gcggagggcc
     3961 tgccggacca gaacatcttc gacttctcgc tctacatcct cgcggcggcg atcctggggg
     4021 cgaagctgct cctcgtcatc gtcgagtggc gtcgctacgc ggagaacccc cgcgatctcg
     4081 tggaggtgct ccgatcgggc ggagtcttct acggcgggct gatcgccgcg acggccgtcg
     4141 gcatctggta catgaagaag caccggcttc ccgcatggaa gatcgcagac atgggagcgc
     4201 cctcgatcgc gctcggcgag gcgatcggac gctggggatg cttcgccgcc ggatgctgct
     4261 acggaaggcc gacggactcc ccgctcggcg tgactttcac ggacccattc gcgcacgacg
     4321 cggtcggcac gccgttgaac gttcctctcc acccgaccca gatctatctc tccctgaatg
     4381 cgctcctgat cttcctgatc ctgcagtggg tctaccgccg gcgtacgttc gacggcgaag
     4441 tcttctggct ctacgtgctc ctctatgcga tcacccgagg gattctcgag accttccgcg
     4501 gcgactccat acgcggcttc ctgattcccg gggttctctc gacttcgcag ttcatcggcg
     4561 tcctcgcggc cgtcgcagcc gccggaatgc tcgtcattct ttcgcgccgg cgccgcgccg
     4621 cggcgtcgtc gagttgatcc gccgcttccg ggtcggaacc ccgtccgaag gcacgcgtct
     4681 cgacctcttt ctcgcgtccg cgtgctcgga cctctcccga tcccggatcc agaagttgat
     4741 cgccgaaggc gcggtcacgc tcgacggcgt ccccgccaaa cgatcgcacg tcgtccgcgc
     4801 cggcgggcgc gtcgaggtcg acgttccgga gccccgcgcg gcggcgatcg agccggagga
     4861 gatcccgctc tcgatcctct atgaggacga gcacctcctc gcgatcgaca agccgtccgg
     4921 cctcgtcgtc catccctcac cgggacacga ctcgggcacc ctcgtcaatg ccctgctcca
     4981 ccacgttcgc gacctctcgg ggatcggcgg ggaactgcgc ccgggcattg tgcaccggct
     5041 cgaccgggac acgtcgggcg tcctcctcgt cgccaagtcc gaccgggcgc acgcatcgct
     5101 gtcccggcag atgaaaaatc gcgcgctgaa gaaggagt
//
LOCUS       DASWAV010000095         3314 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U2665
            k141_685172, whole genome shotgun sequence.
ACCESSION   DASWAV010000095 DASWAV010000000
VERSION     DASWAV010000095.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960316
            Sequence Read Archive: SRR10260007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 3314)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3314)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:47:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,929
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3314
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_685172"
                     /isolate="SMAG_U2665"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.36444 N 132.31889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1213)
                     /locus_tag="VF958_02025"
     CDS             complement(<1..1213)
                     /locus_tag="VF958_02025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9147917.1"
                     /translation="MTNRVTNRFVRALLLGGIGAVLGESAVLAQIGATVPNWTVPPYS
                     ARSGGSGITTMTDISDGSVFVAVTPCRVVDTRGAAGPYGGPALTANVARTFDIDSGPC
                     AGIPAGSSAYSLSIGAILPPANGFLAAWPTGTAQPPVSQLNFLTGQVIANAAIVPGGT
                     NGSIDVLVNVGPTHIYIDINGYFMDSGGTLNTGRQLLWNGTFAGNFMQITNSDAATQN
                     ATAIKGIMNTGQAGATAVAGVQLGVAGRTYGVRGSVAADPVSLSSQNGAGVLGTGPNA
                     GAVDGVLGVISAPNGGAAVRGVRTNVTPPTSGALGLFGRGGLFENTVEVIDGDLLLTL
                     LNSTTKANIDIDGNLTVAGTKSFIEPHPTDASKAIKYVSLEGPESGTYFRGRARFQNG
                     MARIAVPEDFRL"
     gene            complement(1295..2272)
                     /gene="gmd"
                     /locus_tag="VF958_02030"
     CDS             complement(1295..2272)
                     /gene="gmd"
                     /locus_tag="VF958_02030"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012875651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008446 - GDP-mannose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HEX9147918.1"
                     /translation="MKSALITGINGQDGSYLAEHLLAHGYRVCGLVRRTSIRNLERVS
                     HLLEQLELLNGDLLDQNSLINAVAAAQPDEVYNLAAQSFVPASFSQPVLTGEFTALGV
                     TRMLEAIRIVNPRIRFYQASSSEMFGKVHSVPQDEETKFHPRSPYGVAKLYGHWITVN
                     FRESYGMFAVSGILFNHESPRRGTEFVTRKITLAAARIAAGKQKELKLGNLEAQRDWG
                     FTGDFVEAMRLMLEQPEPRDYVIGTGETHTVQEFVEAAFGRVGLDWHKYVVIDPKLVR
                     PAEVDLLISNPRRAMEELGWKPRVSFHELVRMMVDADVAATAREIPSAV"
     gene            2340..>3314
                     /locus_tag="VF958_02035"
     CDS             2340..>3314
                     /locus_tag="VF958_02035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9147919.1"
                     /translation="MASRAPALTTPRLARGARKETASPGASFIAGKLSDNFWIILSPF
                     VALAVLASLWKWSGQSDMTVYSILFGFIVTGHHMPGWIRAFGEPAVYERHKVRLWVSL
                     LAIPPLMILPAFFGLGAVGLTIAAAFDLWHVAMQQHGFGRIYAAKAGDVERRSARLDL
                     ACVLTWYATVVAWSDSWMEGIARSFRKAGLPIFDRVTPPGWAAVRWGLLAVSVLLLAA
                     YVANAISIWRRKKIVTPNKHLLHLAAFSVLIWTYQYSSWFRAQSVQNLFHASQYFFMV
                     WVYGNLSIRRDATRPGNLYRALFQTRKGTLLFGGFVLLYGIGAYLLSSS"
BASE COUNT          524 a         1089 c         1068 g          633 t
ORIGIN      
        1 cgagtcggaa atcctcgggc actgcaatcc gggccatgcc gttctggaac cgcgcccgtc
       61 cgcggaagta ggtcccggat tccggaccct cgagggagac gtacttgatc gccttggagg
      121 cgtcggtcgg gtgcggctcg atgaaggact tcgtccccgc gaccgtgagg ttgccgtcga
      181 tgtcgatgtt cgccttggtc gtggagttga gtagcgtcag cagaaggtcg ccgtcgatga
      241 cctcgaccgt attctcgaag aggccacccc ggccgaacag cccgagcgct ccggacgtcg
      301 gcggtgtgac gttcgttcga actccgcgca cggcagcgcc tccattcggc gccgagatga
      361 cgcccaacac gccgtcgacc gcgcccgcgt tcggccccgt gcccagcact ccggccccgt
      421 tctgcgatga cagggagacg gggtccgcgg ccacgcttcc gcggacgcca tacgtgcgcc
      481 ccgcgacccc gagctgcacc ccggccaccg ctgtggctcc ggcctgaccg gtgttcatga
      541 ttcccttgat cgccgtggcg ttctgggtcg cggcgtcgct gttcgtgatc tgcatgaagt
      601 tgccagcgaa cgtcccgttc cagaggagct gcctccccgt attcagagtt cccccggaat
      661 ccatgaagta cccgttgatg tcgatgtaga tgtgagtcgg cccgacgttg acgaggacgt
      721 cgatcgagcc gttggtcccg ccgggaacga tggccgcgtt ggcgatgact tgaccggtga
      781 ggaaattcag ctgggagacc ggcggctgcg cagtccccgt cggccaggcg gcgagaaacc
      841 cgttcgcggg aggaaggatc gccccgatgc tcagggagta tgccgagctc ccggcgggga
      901 tccctgcgca cgggccgctg tcgatgtcga aagtccgtgc gacgttcgcc gtgagcgccg
      961 ggccgccgta gggcccggcg gcaccgcggg tgtcgacgac gcgacagggc gtgacagcga
     1021 cgaacactga gccgtcggag atgtcggtca tcgtcgtgat gcccgaccct ccggaccgcg
     1081 ccgagtaggg tggtacggtc cagttcggaa ccgtcgcgcc gatctgcgcg agtaccgcac
     1141 tctcgccgag cacggcgccg attcctccga ggagcagagc gcggacaaac cgattcgtca
     1201 ctcgattggt catggaatga ctcctttctt cggcggaatc cgaaaacgtc cgccgcgaga
     1261 ctaacagcaa cgacgtccgg cgaagaaagc gccgtcaaac ggcgctgggt atttcccgcg
     1321 cggtggccgc cacgtcggca tctaccatca ttcgcacgag ctcgtgaaag ctgacgcggg
     1381 gcttccagcc gagctcttcc attgcacgac gcggattcga gatcagaagg tcgacctcgg
     1441 ccgggcgaac gagcttcggg tcgatgacga cgtacttgtg ccaatcgagt ccgacgcgcc
     1501 cgaacgccgc ctcgacgaac tcctgcaccg tgtgcgtctc accggtaccg atgacgtaat
     1561 ccctgggttc cggttgctcg agcatcaggc gcatggcttc aacgaagtct cccgtgaacc
     1621 cccagtcgcg ctgcgcctcg aggttgccga gcttgagttc cttctgcttg cccgcggcga
     1681 ttcgcgcggc cgcgagcgtg atcttgcgcg tcacgaattc cgttccgcgc cggggcgact
     1741 cgtggttgaa caggatgccc gacaccgcga acattccgta ggactcccgg aagttcacgg
     1801 tgatccagtg cccgtagagc ttcgcgaccc cgtacgggct tcgcgggtga aacttggtct
     1861 cctcgtcctg cggcaccgag tgaacttttc cgaacatctc ggaggaggac gcctggtaga
     1921 agcggatccg gggattcacg atccggattg cctcgagcat cctggtgact ccgagggccg
     1981 tgaactcccc cgtcaggacc ggctgggaaa aggatgccgg cacgaacgac tgcgcggcga
     2041 ggttgtagac ctcgtccggc tgagcggccg cgaccgcatt gatcaacgag ttctggtcca
     2101 ggagatcccc gttcaggagc tcgagctgct cgagcaggtg ggagacgcgc tcgaggttcc
     2161 ggatgctggt ccgtcgcacg agcccgcaca cgcggtatcc gtgggcgagc aggtgttccg
     2221 cgaggtacga gccgtcctgt ccgttgatac ccgtgatgag cgccgatttc attccgatct
     2281 cccgagacgt ctcgcgcgag tatatgagtt cgagctctcg gccgtcttag aatcgtcccg
     2341 tggcttctcg tgctccggcg ctgacgaccc cgcgacttgc gcgcggcgca cggaaagaga
     2401 ctgcctctcc cggcgcctct ttcatcgcgg gcaagctctc ggacaacttc tggatcatcc
     2461 tgtcgccctt cgtcgcgctc gcagttcttg cgtcgctctg gaagtggtcc gggcagtcgg
     2521 acatgacggt ctactcgatt ctcttcgggt tcatcgtgac cggccaccac atgcccggct
     2581 ggatccgcgc cttcggggaa cccgccgtct acgagagaca caaggtccgc ctgtgggtct
     2641 ctctcctcgc aatcccgcca ctcatgattc tcccggcttt cttcggcctc ggggcggtcg
     2701 ggctcacgat cgcggccgcc ttcgacctct ggcacgtcgc gatgcagcag cacggtttcg
     2761 gaaggattta tgcggccaag gccggcgacg tcgaacggag gtcggcgcgt ctcgacctcg
     2821 catgcgttct gacctggtac gcgacggtcg tggcgtggtc ggactcgtgg atggagggga
     2881 tcgcgcgttc gttccgcaag gcgggcctgc cgattttcga ccgggtgacc ccgccgggat
     2941 gggcggccgt ccggtgggga cttctggctg tgagtgtcct gctcctcgcg gcgtacgtcg
     3001 cgaacgcgat ctcgatctgg cggcgcaaga agatcgtgac gccgaacaag catctcctcc
     3061 atctcgccgc cttctcggtg ctcatctgga cgtaccagta ctcgagctgg tttcgcgcgc
     3121 agtccgtcca gaatctcttc cacgcctccc agtacttctt catggtctgg gtatacggga
     3181 acctgtcgat ccgcagggac gcgacgcggc cggggaacct ctatcgcgcc ctcttccaga
     3241 cgaggaaggg cacgctcctc ttcgggggat tcgtcctcct gtacgggatc ggcgcttacc
     3301 tcctctcttc gtcc
//
LOCUS       DASWAV010000096         3707 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U2665
            k141_404957, whole genome shotgun sequence.
ACCESSION   DASWAV010000096 DASWAV010000000
VERSION     DASWAV010000096.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960316
            Sequence Read Archive: SRR10260007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 3707)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3707)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:47:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,929
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3707
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_404957"
                     /isolate="SMAG_U2665"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.36444 N 132.31889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..758)
                     /gene="rsmA"
                     /locus_tag="VF958_02040"
     CDS             complement(<1..758)
                     /gene="rsmA"
                     /locus_tag="VF958_02040"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: protein motif:HMM:TIGR00755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HEX9147920.1"
                     /translation="MIPARKRWGQHFLVRAETARRIVEASGVGPGDTAFEVGPGEGAL
                     TRPLAERAGRVLAVEIDPLRTEMLAPALPANVRLLCGDVLDRSYAEWLAAVGWSPPAV
                     LVANLPFNAATPILVRAIEEEGTFSRAIATVQKEVARRFSAAPGSKAYGYLSVRAAAF
                     ARGRVLFDLPPGAFRPSPRVTSSVLELVPLTGPVDRAGRDRALAIASLAFRFRRKTVA
                     NALSSAVDRARVTRALEELGRSPMARAEELSLAD"
     gene            complement(755..1807)
                     /locus_tag="VF958_02045"
     CDS             complement(755..1807)
                     /locus_tag="VF958_02045"
                     /inference="COORDINATES: protein motif:HMM:NF013694.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HEX9147921.1"
                     /translation="MALLREIDPGELGLAGRVKPAERNPPRTVLFDGSGARESGVPMD
                     GITTRLAAGGLFFFADLHAPDEQDLVSLQREFHFHPLAIEDVRTRHQRPKIDIYGDQY
                     FLVFYRVGPSAEKDGDWVLQELDFFIGPNFLIVTHDSPVDLLDMTFERFCREEGKKDV
                     SGLLYDMLDTLVDDYFLFLDDLGERSQEIEDDIFEHYDQSHLERLLALKKDLALLRRI
                     AAPERDAVNVLLRRDPPILDAARIFYFQDIYDHLIRIADSIDTYRELATGTLDAFLSV
                     QNNQLSEVVRKLTIISTIFLPVMLVTSFFGMNFHWLSNASNAIFAKGMAFMALFEVAI
                     FLFLRYWGILGRRRAG"
     gene            1855..3012
                     /locus_tag="VF958_02050"
     CDS             1855..3012
                     /locus_tag="VF958_02050"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor domain-containing diguanylate cyclase"
                     /protein_id="HEX9147922.1"
                     /translation="MLQVYTLDNRRLENFLERHKAYYAYTGEIEIDRFLREILQKANE
                     FVPSEAGSILLDDPMTKNSDDRMENDLVFIATYGARANGRLSRRMNARIGIVGHVYAT
                     GVGYMTEDVVSDPYFYPTLDLPDDFETRSVVCVPIRIEAAVCGVLELVNKIGPGGFRE
                     KDRRLLEIFADYASISIQNLLDARRAFEVAKRDGLTGLFNERYLHLRLTQELSRISRD
                     GGDLCVVFLDLDNLKSVNDRHGHLAGSQVLREVGYLLRRTITDDRATLTRYGGDEFVL
                     ILPGHDMKSGMEVAQSIRNAIRSSTFLSRPYGFNDPALDLQGVLTGSLGVSSIRSNAT
                     AGASVEQQKNELLRAADAALYRAKQSGKDRCVGARAEDSRFAPAQSVAGGG"
     gene            3061..>3707
                     /locus_tag="VF958_02055"
     CDS             3061..>3707
                     /locus_tag="VF958_02055"
                     /inference="COORDINATES: protein motif:HMM:NF012428.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEX9147923.1"
                     /translation="MISTTRIELSPQDVLDVLSRHILVDGYHVVMDLERSRGSYLYDS
                     RSDRMLLDFFTNFATYPVGYNHPKTAEPEYRERILLAALNKPTNSDTYTTLYAEFVET
                     FSRLAVPPTHGRHLFFVEGGSLAVENAVKTAFDWKVRKNFARGIAEERGHQILHFRHA
                     FHGRSGYSISMTNTADPRKTQYFPRFDWPRLTCPKLSFPVTADVLRDVAEAEDSV"
BASE COUNT          665 a         1224 c         1204 g          614 t
ORIGIN      
        1 tagtcggcga gcgagagctc ctcggcccgg gccatgggac tgcggccgag ctcctcgagc
       61 gcccgggtga cccgcgcccg gtccaccgcg gaagagaggg cattcgcgac tgtcttgcgg
      121 cggaagcgga aggcaagcga ggcgattgcg agcgcccggt cgcgcccggc gcgatcgacc
      181 ggcccggtca gcgggacgag ctcgaggacg ctcgacgtca cccggggcga tggacggaag
      241 gcccccggcg gcaggtcgaa gagcaccctg ccccgagcga acgccgccgc ccgcaccgag
      301 agatatccgt acgccttcga tccgggcgcc gcggagaatc gccgggcaac ctctttctgg
      361 accgttgcga tcgcgcggga gaacgttccc tcttcctcga ttgcccgcac gaggatcggc
      421 gtcgcggcgt tgaacggcag gttcgcgacg agcaccgcgg ggggactcca gcccaccgcg
      481 gcgagccact ccgcgtagct cctgtccagg acgtcgccgc acaggagccg cacgttggcc
      541 gggagcgcag gcgcgagcat ctcggtccgc aggggatcga tttccacggc aaggacgcgg
      601 ccggcgcgct cggcgagggg gcgcgtgagc gcgccctccc cggggccgac ttcgaaggca
      661 gtatcgcccg gaccgacccc ggacgcttcc acgatgcggc gcgcggtctc agcgcggacg
      721 aggaagtgct gcccccagcg cttgcgcgcc gggatcaccc ggctctcctc cgcccgagaa
      781 ttccccagta gcgcaggaag agaaagatgg cgacctcgaa gagggccatg aaagccatgc
      841 ccttggcgaa gatcgcattg ctggcattgc tcaaccagtg gaagttcatt ccgaagaaac
      901 tggtcacgag catgaccggg aggaagatcg tcgaaatgat cgtgagcttt cggacgacct
      961 cggagagctg gttgttctgc accgagagaa aggcgtcgag cgttcccgtc gcgagctcgc
     1021 gataggtgtc gatcgagtca gcaatccgga tcaggtggtc gtagatgtcc tggaagtaga
     1081 agatgcgcgc cgcgtcgaga atcggcggat cgcgtctcag caggacgttg acggcgtccc
     1141 tctcgggagc ggcgatccga cgcagcagcg cgaggtcctt cttcaaggcg aggagccgct
     1201 cgagatggct ctgatcgtag tgttcgaaga tatcgtcctc gatctcctgc gaccgctccc
     1261 ccaggtcgtc gaggaagagg aagtagtcgt cgacgagcgt gtcgagcatg tcgtagagga
     1321 gtcccgagac gtcctttttc ccctcctcgc ggcagaagcg ctcgaacgtc atgtcgagca
     1381 gatcgaccgg cgagtcgtgc gtgacgatga ggaagttcgg gccgatgaag aagtcgagct
     1441 cctggaggac ccagtcgccg tctttctccg cgctcggccc gacccggtag aagacgagga
     1501 agtactggtc gccgtagatg tcgatcttcg ggcgctggtg ccgggtccgg acgtcttcga
     1561 tcgcgagcgg gtggaagtga aactcccgct ggagcgacac gagatcctgc tcgtcggggg
     1621 cgtgcagatc ggcgaagaag aacaggccgc ccgccgcgag ccgcgtcgtg attccgtcca
     1681 tcggaacgcc gctctcgcgc gctcccgagc cgtcgaagag gacggttcgc ggggggttgc
     1741 gttcggccgg tttgacgcgc ccggcgagcc cgagctcccc ggggtcgatc tcgcgcagga
     1801 gcgccacgaa acgagcgtat cagatggtag aatcagtggg ttccggcgat tctcgtgctg
     1861 caagtctaca cactcgacaa ccggcgtctc gagaactttc tcgagagaca caaggcttac
     1921 tacgcctaca ccggcgagat cgagatcgat cgtttcctcc gcgagattct gcagaaggcg
     1981 aacgagttcg tcccgtcgga ggcgggctcg attctgctcg acgacccgat gacgaagaac
     2041 tccgacgatc gcatggagaa cgacctcgtc ttcatcgcga cgtacggcgc gcgcgcgaat
     2101 ggacgcctct cccgccggat gaacgcccgg atcgggatcg tcggacacgt ctatgcgacc
     2161 ggcgtcgggt acatgaccga agacgtcgtc tccgatccct atttctatcc gacgctcgac
     2221 ctgccggacg acttcgaaac gcgctcggtc gtctgcgttc cgatccgcat cgaggcggcc
     2281 gtgtgcggcg tgctcgagct cgtgaacaag atcggccccg gtggcttccg cgaaaaggat
     2341 cggcggctgc tcgagatctt cgccgactac gcctcgatct cgatccagaa cctgctcgat
     2401 gcgcggcgcg ccttcgaagt cgcgaagcgt gacggcttga ccggtctctt caacgagcgc
     2461 tacctccacc tccgcctgac ccaggaactc tcgcgcatct cgcgcgacgg cggcgacctc
     2521 tgcgtcgttt tcctcgacct ggacaatctg aaatccgtga acgaccggca cgggcacctc
     2581 gcgggcagcc aggtgctgcg ggaggtcggg tatctcctcc gccgcacgat caccgacgac
     2641 cgggcgacgc tgacgcgcta cggcggcgac gagttcgtgt tgatcctccc cgggcacgac
     2701 atgaagagcg ggatggaggt cgctcagtcg attcgtaacg cgatccgctc ctccactttc
     2761 ctctcgcggc cgtacgggtt caacgatccc gcgctggacc tccagggcgt cctgacgggg
     2821 tcgctcggcg tctcctcgat ccgatcgaac gcgaccgcgg gagcgtcggt cgaacagcag
     2881 aagaacgagc tccttcgcgc agccgacgcg gctctctatc gggccaaaca aagcggcaag
     2941 gaccggtgcg tcggtgcgcg ggcagaagac agccgattcg ccccggctca gtcggtcgct
     3001 ggagggggct aactccgact ccgtgttaga cttcgcggtt ccgaatcgac gaggagccgc
     3061 atgatttcca ccacccggat cgagttgtcg ccgcaggacg tcctcgacgt cctttcccgt
     3121 cacatcctgg tcgacggcta ccacgtcgtc atggacctcg agcggagccg cggctcgtat
     3181 ctctacgact cgcgcagcga ccggatgctc ctcgatttct tcaccaactt tgcgacctac
     3241 ccggtcggct acaaccaccc gaaaacggcc gagcccgagt atcgcgagcg gatcctgctc
     3301 gcggcgctca acaaaccgac caacagcgac acctacacga cgctctacgc cgagttcgtc
     3361 gaaaccttct cgaggctcgc cgtgccgccg acgcacggcc gccatctctt cttcgtcgag
     3421 ggcggctccc tcgcggtcga gaacgccgtg aagaccgcct ttgactggaa ggtccggaag
     3481 aatttcgcgc gcggaatcgc ggaggagcgt ggccatcaga tcctccactt ccgacatgcc
     3541 ttccacggcc ggtccggata ctcgatctcg atgacgaaca cggccgaccc ccggaagact
     3601 cagtactttc cgaggttcga ctggccgcgg ctcacctgcc cgaagctgtc gtttcccgtc
     3661 acggccgacg tcctgcgcga cgtggccgaa gccgaggaca gtgtcga
//
LOCUS       DASWAV010000097         3307 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U2665
            k141_165260, whole genome shotgun sequence.
ACCESSION   DASWAV010000097 DASWAV010000000
VERSION     DASWAV010000097.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960316
            Sequence Read Archive: SRR10260007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 3307)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3307)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:47:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,929
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3307
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_165260"
                     /isolate="SMAG_U2665"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.36444 N 132.31889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..205
                     /locus_tag="VF958_02060"
     CDS             <1..205
                     /locus_tag="VF958_02060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9147924.1"
                     /translation="QYDPPGTGGASYAGTGNYYLVFEGKEGDATAHYRFGVTRQQYIR
                     FEDGAHVQLVIVDGNLKDIRPLR"
     gene            202..693
                     /locus_tag="VF958_02065"
     CDS             202..693
                     /locus_tag="VF958_02065"
                     /inference="COORDINATES: protein motif:HMM:NF024822.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4112 domain-containing protein"
                     /protein_id="HEX9147925.1"
                     /translation="MKPEELAKRTLEPEVVPPDVPDPWIEKLAWVMDSSIPIGRWRIG
                     LDGIIGLVPGLGDLAGAVISALIVVAGVRAKLPRSAIARMVANVAIEAVVGAVPFLGD
                     LFDMAWKANMRNVEIFREALHGQRSRRRDTLFVTGLLLALAAIVAFPVVVIVLLLRQF
                     GLL"
     gene            complement(722..2287)
                     /locus_tag="VF958_02070"
     CDS             complement(722..2287)
                     /locus_tag="VF958_02070"
                     /inference="COORDINATES: protein motif:HMM:NF024579.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX9147926.1"
                     /translation="MRRSVSLFASALAVATVLAAQEKLLDPHAGCTGPSGESVDPAML
                     ERPTSIHAGAGKVTQKITAKTPSVQAFYDQGLAYLHSYVWIEAARSFHQALREDPDCA
                     MAWMGLARAEQGLNRDDASKAAIEKAKSLEMKVSESERRHIELRAQQIDAQLAPADKE
                     KEKHESYKRAIEKAIAADPSDAELWILRGNAEEPGPWGRGQAGGIGAIAFYEAALARY
                     PGHLGAHHYLVHAYENVSRHADAAEHGKIYASAAPGVAHAQHMYGHVLPRLGRWKEAL
                     AQFEKADAIEEAYAREEHLRPGDDWHHVHNLQLLGYTYLRLGRFDEAEKAFGRAFNTP
                     IRYPRYGREQATIAEYLLLRGRVDEALAAARATANRSGAARAAGAAVEGEALLALGRR
                     AEAIEASKRARAALDEAKKTAGYDAVWLERLIDPHIRQLEAEIALTDKASADSEEALR
                     RIADELAANPRFDAWGEGLFRLERIAEQAKRSGRDALAREIQERMRRIDPDYRPGSTA
                     LPISAPAVASSSR"
     gene            2343..>3307
                     /locus_tag="VF958_02075"
     CDS             2343..>3307
                     /locus_tag="VF958_02075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011521191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEX9147927.1"
                     /translation="MTRRSNPLGAFRPPVRRWFEASFAAPTAAQAKGWPSIVAGRSTL
                     LLAPTGSGKTLAAFLAAIDRLMFSPEPRKKERARILYVSPLKALAVDVERNLRAPLAG
                     IAAAAGAAGETFRIPAVAIRSGDTPAGDRARMARTPPDIIITTPESLYLMLTSAAREV
                     LRSVDTVIVDEIHSVVASKRGAHLFLSLERLEALRSGAKPLQRIGLSATQRPLEEVAR
                     LLGGGEPGGGRGGWKPRPVEIVDAGSRKAFEVTVEVPVEDMARLGEVLETPSGPAAGP
                     PARSSIWPSIHPRLVELVRAHRSTMIFVNSRRLAERLASALNETAG"
BASE COUNT          450 a         1136 c         1159 g          562 t
ORIGIN      
        1 gcagtacgac ccaccgggca cgggcggcgc gagctacgcg ggcacgggga actactacct
       61 ggtcttcgag gggaaggaag gggacgcgac cgcgcactat cgttttggcg tgacgcgaca
      121 gcagtacatc cgcttcgagg acggggcgca cgttcagctc gtcatcgtcg acggaaacct
      181 gaaggatatt cggccgcttc ggtgaaaccg gaggagcttg cgaagcgaac cctcgagccg
      241 gaggtcgtgc cgcccgacgt cccggacccc tggatcgaga agctcgcctg ggtcatggat
      301 tcttcgatcc cgatcgggag gtggcggatc gggctcgacg ggatcatcgg gctggtgccc
      361 gggctgggag acctcgccgg agccgtgatt tccgcgttga tcgtcgtcgc gggcgtccgc
      421 gcgaagctgc cgcggtcggc gatcgcgcga atggtcgcca acgtcgcgat cgaagcggtc
      481 gtgggcgccg tgcccttcct cggagatctc ttcgacatgg cgtggaaggc gaacatgagg
      541 aacgtcgaga tctttcgcga agcgctccac gggcagcgca gccgccgccg cgacactctc
      601 ttcgtcacgg gtctcctcct ggcgctggcg gcgatcgtcg cgttccccgt cgtcgtgatc
      661 gtcctgctgc tccggcagtt cgggttgctg tagaggcccg tcggccggcg acggcgagcg
      721 gtcagcggct cgagctcgcc acggccggcg cactgatcgg cagggcggtc gagccgggcc
      781 gatagtccgg atcgatgcgc cgcatccgct cctggatctc ccgggcgagg gcgtcgcgcc
      841 ccgacctctt cgcctgctcc gcgattcgtt ccagccggaa gagcccttct ccccaggcgt
      901 cgaaccgcgg attggccgcg agctcgtcgg caatccggcg cagcgcttcc tcggagtcgg
      961 ccgatgcttt gtcggtgagc gcgatctccg cctccagctg gcggatgtgg gggtcgatca
     1021 ggcgttccag ccagacggcg tcatatccgg cggtcttctt cgcctcgtcg agcgcggccc
     1081 gggcccgctt cgaagcttcg atggcctcgg cccgccgacc cagtgcgagc agagcctccc
     1141 cctcgacggc ggccccggcc gcgcgcgcgg cgccgctccg gttcgcggtc gcgcgggcag
     1201 cagcgagcgc ctcgtccacg cggccccgca ggagcaggta ttcggcgatc gtggcctgct
     1261 cccggccgta gcgcgggtag cggatcggcg tgttgaacgc gcgcccgaac gccttctcgg
     1321 cctcgtcgaa tcgaccgagc cggaggtacg tgtacccgag gagctgaagg ttgtggacgt
     1381 gatgccagtc gtcgccaggg cgcaggtgtt cctcgcgcgc gtaggcctct tcgatcgcgt
     1441 ccgccttctc gaattgcgcg agcgcctcct tccaccgacc gaggcgcggc aacacgtgtc
     1501 cgtacatgtg ctgcgcatgg gcgacgcccg gcgccgccga cgcgtagatc tttccgtgct
     1561 cggcggcgtc ggcgtggcgg ctgacgttct cgtatgcgtg gacgaggtag tggtgagcgc
     1621 cgaggtgtcc gggatatcgc gccagcgcgg cttcgtagaa cgcgatcgca ccgatcccgc
     1681 ccgcctgccc gcgtccccat ggtccgggct cctcggcgtt gccgcgcagg atccagagct
     1741 cggcgtccga cggatccgcc gcgatcgcct tctcgatggc gcgtttgtaa gactcgtgct
     1801 tctccttttc cttgtccgcc ggcgcgagct gcgcgtcgat ctgctgagcg cggagctcga
     1861 tgtgccgacg ctccgactcg ctgaccttca tctcgagtga cttcgccttc tcgatcgcag
     1921 ctttcgatgc gtcgtcgcgg ttcaagccct gctccgcgcg cgcgagaccc atccaggcca
     1981 tcgcacagtc gggatcctcg cggagcgcct ggtggaacga gcgagccgcc tcgatccaga
     2041 cgtacgagtg gaggtacgcg agaccctggt cgtagaacgc ctggacgctc ggcgtctttg
     2101 ccgtgatttt ctgcgtgacc ttgcccgcgc ctgcgtggat tgaggtcggc cgttcgagca
     2161 tcgcgggatc gaccgactcg cccgacggcc cggtgcagcc ggcgtgcggg tcgagcagtt
     2221 tttcctgagc ggcgagaacc gtcgcgaccg cgagcgcgga cgcgaagagc gagacggagc
     2281 gtctcatgcg gtctcctttc gaacgccgaa tcttagctcc ggagtcgagg gagaatgcgc
     2341 cggtgacccg gcgttccaat cctctcggtg cgttccggcc ccccgtacgg cgctggttcg
     2401 aggcctcctt cgcggcgccg acggcggcgc aggcgaaggg gtggccatcc atcgtcgccg
     2461 gacggtcgac gcttctcctc gcgccgaccg gctccggaaa gacgctcgcg gctttcctcg
     2521 cggcgatcga ccgactgatg ttctcgcccg agccgcggaa gaaggagcgc gcgcgcattc
     2581 tgtacgtctc gccgctgaag gcgctcgcgg tggacgtcga gagaaatctg cgggcgccgc
     2641 tcgcgggcat cgcggccgca gcgggcgccg ccggcgaaac attccgcatt ccggcggtcg
     2701 cgatccgttc cggggatacg cccgccggcg accgggcgcg catggcgcgg actccgcccg
     2761 acatcatcat cacgacgccg gagtcgctct acctgatgct cacttcggcg gctcgcgaag
     2821 tcctgcgatc cgtggacacg gtgatcgtcg acgagatcca ctcggttgtc gcctcgaagc
     2881 gcggcgcgca tctcttcctc tcgctcgaac gtctcgaggc gctgcgcagc ggcgcgaaac
     2941 ctctccagcg catcggcctt tcggcgaccc agcgcccgct cgaggaggtc gcccggctcc
     3001 tcggcggtgg tgagccggga ggaggtcggg gagggtggaa accgcgcccg gtcgagatcg
     3061 tggacgcggg gagccgcaag gccttcgagg tgaccgtgga ggtgccagtc gaggacatgg
     3121 cgagattggg cgaagtgctc gagacgccgt ccggcccggc cgcgggtccg cccgcgcgaa
     3181 gctcgatctg gccgtcgatc cacccgcggt tggtcgagct cgtccgcgcg caccgctcca
     3241 cgatgatctt cgtcaacagc cgccggctcg ccgagcgact cgcgtccgcc ctgaacgaga
     3301 cggcggg
//
LOCUS       DASWAV010000098         1795 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U2665
            k141_324310, whole genome shotgun sequence.
ACCESSION   DASWAV010000098 DASWAV010000000
VERSION     DASWAV010000098.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960316
            Sequence Read Archive: SRR10260007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 1795)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1795)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:47:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,929
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1795
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_324310"
                     /isolate="SMAG_U2665"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.36444 N 132.31889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..617)
                     /locus_tag="VF958_02080"
     CDS             complement(<1..617)
                     /locus_tag="VF958_02080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014099760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEX9147928.1"
                     /translation="MIIAIANQKGGVGKTTTAINLAAALAQKGMKTLLIDLDPQGNST
                     MSFLDRRSIGKSMFDVLTEKDAMMSHIVQHTGMTNLDVAPARIALAKIESKLLGEIDG
                     HFRLRDKLDGARKRYDAIVIDTPPTLGMITVNAMVAATHILIPIQSSYFALEGTDDLL
                     ETIDKIKARPNPALSLLGVVITLHDRRTVIAKDVYDQIRTVFGEKL"
     gene            complement(700..1182)
                     /locus_tag="VF958_02085"
     CDS             complement(700..1182)
                     /locus_tag="VF958_02085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018129813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional nuclease family protein"
                     /protein_id="HEX9147929.1"
                     /translation="MIKMEIKGLLMDPVSNMPVVVLRDSANGLFLPIWVGIFEANAIA
                     LQMEKITTPRPMTHDLLKNLLSELDAKVERIVINDLKDNTFFARIHLLRGDVRWNVDS
                     RPSDAIALALRVDAEIFVEEDVLERSKSLRPEGDEGDPDRLRKWLEEVNPDELGKYQM
                     "
     gene            complement(1193..>1795)
                     /locus_tag="VF958_02090"
     CDS             complement(1193..>1795)
                     /locus_tag="VF958_02090"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HEX9147930.1"
                     /translation="LDDSMIDAIAGGKNVAPYLHLPAQSGSDRVLYRMKRRYTAAEYL
                     EKVGRVRAALPGIAISSDFIVGFPGETDEDFEATLRLVSEARFSNLFAFRYSARAGTA
                     AARWGKETSVAEELAAERLARVLALQEAIQQEINESLVGGEFEILVEGAGRDGKIRGR
                     TPCNRITHVDAGSRELKPGEYVRVLITRGLPNSLLAEIAA"
BASE COUNT          296 a          568 c          586 g          345 t
ORIGIN      
        1 agcttctccc cgaagaccgt ccggatctgg tcgtagacgt ctttcgcgat gaccgtccgc
       61 cggtcgtgga gcgtgatcac gacgccgagc agcgacaggg cggggttcgg gcgcgccttg
      121 atcttgtcga tcgtctccag cagatcgtcc gtgccttcga gcgcgaagta ggacgactga
      181 atggggatca ggatgtgcgt cgccgcgacc atcgcgttga ccgtgatcat gccgagcgtc
      241 gggggcgtgt cgatgacgat cgcatcgtat ctcttgcgcg cgccgtcgag cttgtcgcgc
      301 agccggaaat gcccgtcgat ctcaccgagg agcttcgact cgatcttggc gagcgcgatc
      361 cgcgccggcg caacgtcgag gttggtcatg cccgtgtgct ggacgatgtg cgacatcatc
      421 gcgtccttct cggtcagcac gtcgaacatg gatttcccga tcgagcgccg gtcgaggaac
      481 gacatcgtgc tgttgccctg ggggtcgagg tcgatgagaa gggttttcat ccccttctgg
      541 gcgagggcgg ccgcgagatt gatcgccgtg gtcgtcttcc cgacgccccc cttctggttg
      601 gcgatggcga tgatcacgga atcacagtgt atcgcgaaga aacgaaaacg cgcgcggaaa
      661 gccgcgcgcg tgagcggaag ggagggcgaa cgccgacctt tacatctgat actttccgag
      721 ctcgtcgggg ttgacctctt ccaaccactt tctcagacga tcgggatccc cttcgtcccc
      781 ttcgggtcga agactcttgc tcctctccag cacgtcctct tcgacgaaga tctccgcgtc
      841 gacgcgcagc gcgagcgcaa ttgcgtcgct cggccggctg tcgacgttcc accgcacgtc
      901 ccctcggagc aagtggattc gcgcgaagaa ggtgttgtcc ttgagatcgt tgatcacgat
      961 ccgttcgact ttggcgtcga gctccgagag gaggttcttc aacaggtcgt gcgtcatcgg
     1021 gcggggcgtc gtgattttct ccatctggag ggcgatcgcg ttcgcctcga agatgcccac
     1081 ccagatgggc agaaagaggc cgttggcgga gtcgcgcagg acgacgaccg gcatattcga
     1141 caccgggtcc atcagcaggc ctttgatctc catcttgatc atcgtgcgcc ccctacgcgg
     1201 cgatttccgc cagaagagag ttgggcagcc cccgggtgat gagaacccgg acgtactcgc
     1261 ccggtttcag ctcccgcgat cctgcgtcca cgtgggtgat gcggttgcaa ggcgtgcgcc
     1321 cccggatctt tccgtcgcgg ccagccccct cgacgaggat ctcgaactcc cccccgacga
     1381 gggattcgtt gatttcctgc tggatcgcct cctggagcgc gaggacccgg gccagccgct
     1441 cggcggccag ctcttccgcg acggacgtct ccttccccca gcgcgccgcg gcagtcccgg
     1501 cccgggccga gtagcggaaa gcgaacaggt tggagaaccg cgcctccgaa acgagccgga
     1561 gcgtcgcctc gaagtcctca tcggtctcgc cgggaaagcc gacgatgaag tccgacgaga
     1621 tcgcgatccc gggcagagcg gcgcggacgc ggccgacctt ctcgaggtat tcggcggcgg
     1681 tgtaccgcct cttcatgcgg tagaggaccc ggtcggagcc cgactgcgcc ggcaggtgga
     1741 ggtagggagc gacgttcttt ccgccggcga tcgcatcgat catggagtcg tcgag
//
LOCUS       DASWAV010000099        10024 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U2665
            k141_245469, whole genome shotgun sequence.
ACCESSION   DASWAV010000099 DASWAV010000000
VERSION     DASWAV010000099.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960316
            Sequence Read Archive: SRR10260007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 10024)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10024)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:47:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,929
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10024
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_245469"
                     /isolate="SMAG_U2665"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.36444 N 132.31889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1213)
                     /locus_tag="VF958_02095"
     CDS             complement(<1..1213)
                     /locus_tag="VF958_02095"
                     /inference="COORDINATES: protein motif:HMM:TIGR01494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HEX9147931.1"
                     /translation="MTPDPAELGLTERDAAAILEQVGYNELPSAARPSIFRTAFEVVR
                     EPMILMLLAAGAIYLLLGSRREAIVLLFSVLVVIAISVFQNRRTEHALQALRDLSSPR
                     ALVIRDGVRRRIPGREVVPGDLIVLSEGDRVPADATVLEESNLSADESLLTGESVPVA
                     KAVASGHAPARVFSATLVVSGDALARVEATGASTEIGKIGRSLGAIAPGKTRLQEEIG
                     RAVRRIALVGLLLCAGVVVLYGLARGNWLQGLLSGLALAMAILPEEFPVILTIFFALG
                     AWRISRRRVLTRQLPAIESLGAATVLCVDKTGTLTLNQMAVQRVFADGTFSDVSAGAE
                     LPEAARDVVLYGMLASKKDPFDPMEQAIRSLDRRLQAGAESRYSNGEIVRGYPLSPDL
                     LAMSNAWRASRE"
     gene            complement(1242..1694)
                     /locus_tag="VF958_02100"
     CDS             complement(1242..1694)
                     /locus_tag="VF958_02100"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEX9147932.1"
                     /translation="MAAPFRALVALDFSPESLAALKAARALVTRTGGELTVAHVRPLS
                     DVRAAVLEERGDLLRLAAGALAGAIAEHYESRFSRVLGRGKTESIRLLRGDPARELLR
                     ESRRGYDLIVMGNRGRGRVAAFLPGSTVQEMLVRSPIPMLVVPARRRS"
     gene            complement(1706..2104)
                     /locus_tag="VF958_02105"
     CDS             complement(1706..2104)
                     /locus_tag="VF958_02105"
                     /inference="COORDINATES: protein motif:HMM:NF036463.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5335 family protein"
                     /protein_id="HEX9147933.1"
                     /translation="MRTRLIPHAEWFPFFEAFTRRHPGSPTTVWLLGPHIGAQVEARD
                     LPLEGIVADPLGTTITISLGGPAGGNVEHPVPQPMAVWEELTEEGAEQAIGINSADGT
                     TTLVEFRTPISARMVGCVAADAAASSVPSG"
     gene            2218..4953
                     /locus_tag="VF958_02110"
     CDS             2218..4953
                     /locus_tag="VF958_02110"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024938.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX9147934.1"
                     /translation="MEAGILNLASPGRSLDVAPKGAVVPPERYESDVVLKNGSTLHLR
                     PVRPEDAPGLLEFFRGLSPESLYLRFLGVSPFDLARAENLARADYESQFGLVGETRQG
                     IVALGHFHRDLEIPERAEVAFAVADALQGQGIGTSLLDRLARVAREMGIRTFVAEVLP
                     YNRKMIEVFVDSGFAVRQRTEDGVVRFEISLASTAASTERAAERARHAATASMQAFFE
                     PRSVAVVGADRTRGRIGSEILHNLRASGFRGGIFPVNPHADTIQTLRSYPRVTDIPEE
                     VDLAVIVVPATRVDAVVDDCLAKGVKALVVISAGFGETGEAGRAREAALVEKLRSAGV
                     RMIGPNCLGILNTDPKVRLNATFSPVYPPEGRVGMLSQSGALGLAILDYARQLNLGIS
                     TFVSVGNKPDVSGNDLIQYWENDPRTDVILLYLESFGNPRNFGPIARRVSRSKPIVVV
                     KAGRSRAGARAASSHTGALAESDAVVGALLRQSGVIRTRTLEELFDVATLLSHQPLPA
                     GKRVAILTNAGGPGILAADACEALGLSLASLSKETVAALREFLPAEASVGNPVDLLAS
                     ASAEDSRRALRLALADRGVDSLLVIFIPPLVTDADSVAAAVVAEARESSKTVVATFMG
                     AKGAPPGLAPIPCYPFPESAVAALSRATEYAEWRRRPAGTVRELADVRPDDARDFVTR
                     ALERGEGWLTPAEARSVLTAFGIPVARSRIAPGWDEVRRAAREIGYPVALKAIGPTLL
                     HKSDVGGVKLDVANSAALQRAFRDLETRLGDRVTSYLVQEMVPGGVEVIVGAVFDRTF
                     GPLLLYGSGGTLVELLGDTSFRIAPLSDADAADMLNEVKGTARLRGFRGAPPADEKAL
                     TDVLLRVSALLEACPEIQEMDLNPVKVFESGAKVVDFRIRVGTPPAPTRARRISY"
     gene            5028..6077
                     /locus_tag="VF958_02115"
     CDS             5028..6077
                     /locus_tag="VF958_02115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014671083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent alcohol dehydrogenase"
                     /protein_id="HEX9147935.1"
                     /translation="MKAFVMREIGKVGLMEKPIPPVGPNDALVRTTAALICTSDVHTV
                     GGAIGPRQDRTLGHEAVGVIEKLGSAVSGLDVGQRVTVGAITPCWRCENCLRGFPSQC
                     HEALGGWKFANVKDGNLAEFFHVNDAEANVTPIPDEVPDELAVYTCDMMSTGFMGAER
                     AAIPIGGTVAVFGAGPVGLMAIAGARLLGAGLVIAVERPGKRADFARYYGADHVVDFG
                     RLDPAREIGKLTGGKGVDSAIEAVGSAESFASCVKATRPGGTISNVGYHGEGDVVPIP
                     RLAWGVGMADKTIRSGLCPGGRERMERLLRLLQKKRVDPGPLTTHRFRFDEVERAFEM
                     MRTKEDGIIKPLILF"
     gene            complement(6091..8556)
                     /locus_tag="VF958_02120"
     CDS             complement(6091..8556)
                     /locus_tag="VF958_02120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017355175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xylulose 5-phosphate 3-epimerase"
                     /protein_id="HEX9147936.1"
                     /translation="MSASAPRKSLARRAAELRESDPALRAWAEGGGPIRHLPETQVEI
                     AGLAEDLARRGIRGDGVPLFDLLSALDRLARAGMWLVAHQTYARRVHLDGTDLAPSEF
                     KERPEGHMGGSLNMVPGYAGYLAANAISGFTRSWLVGQGHTVSALDSVNLLVGNTTPP
                     HAARYDVSDEGLSRFCEDFYSYRLGPGGVPESPLGSHVNPNTAGGTFEGGYLGFAELQ
                     YIHMPLPGERLVVFLSDGAFEEQRGSDWAPRWWRAEDSGLVAPIMISNGRRIDQRTTM
                     AQSGGVSWFRQHLRLNGFHPIIFDGRDPAAFVWAIFEMERRLEKLSEEVHAGRIEYPV
                     PLPYGIAVAPKGAGFWGAGKLLAHNLPLGANPSRDATAARHFRDSARKLWVPPEELRP
                     ALEKFAAHGESGRVREGDHPLARRDVRALVPPAGNARPISPNRRDPARWTRSSPMTAV
                     DETFLAVVRANPGLRPRVGNPDEMRSNRMLATLDALKFRVTAPEHGLPEAVEGAVITA
                     LNEEAIVCAALGNKGGINIVVTYEAFGMKMHGALRQEIVFSEELRHAGRPPGWLSVPI
                     VLASHAWENAKNERSHQDPSLAEALLGESSDVSRVVFPADANSAAAAILSVFSTRGEI
                     WTLVVPKAESVADAFEHEEAERLANEGAIALDWAGFEPERARLSLTAVGAYQLAEVLK
                     ASFRLAGKGVPHAVVYVSEPGRFRRPRTDGEARHVVPADRVAALWPGSVEARVFVTHT
                     RPEVLLGVLAPEVIGPRPVGLGFSNRGGTLDTRGMLFVNRSSWAHALEAGASLLGTPL
                     DEWLTKDELAALRGEIAPFPVLG"
     gene            complement(8553..9695)
                     /locus_tag="VF958_02125"
     CDS             complement(8553..9695)
                     /locus_tag="VF958_02125"
                     /EC_number="2.7.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015263094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008776 - acetate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006085 - acetyl-CoA biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HEX9147937.1"
                     /translation="MRLLVFNAGSSSLKYRLFELASGDAPAETARGEIERIGEGGEAP
                     PDHAMAVRRVLDDLSRRGEDRFEAYAHRVVHGGERFREPARVDDETLAAIEEATDLAP
                     LHNAPALAVIRECRRTATPPVPQVAVFDTAFHRTLPERAFRYALTLELAQRLGIRRFG
                     FHGLSYRSMCDRYFGETGRRIEPSRLIAFHLGNGASACALRDGRSIDTSMGFTPLEGL
                     VMGTRSGDLDASLVPFIAEREGMTGAEVLAQLNDRSGLLGLSGVSRDVREIEQAADEG
                     NSRARLAIEIFAYRARKYLGAYLAALGGADAILFGGGIGEHAATVRSAMLDGLEELGI
                     SLDQARNERIRGEVGRISAEGSRIEVWVTPTDEEAVIARDAAVLLQ"
     gene            complement(9730..9966)
                     /locus_tag="VF958_02130"
     CDS             complement(9730..9966)
                     /locus_tag="VF958_02130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9147938.1"
                     /translation="MSDALDLTAELLSARLRRAVDLLWRETVQRRRARLDQLERRRSA
                     RRMRNLQKIVRKALADYHRIQVERRDGPPPTRLP"
BASE COUNT         1551 a         3452 c         3500 g         1521 t
ORIGIN      
        1 tctctcgcga cgccctccag gcgttcgaca tcgccaggag gtccggggag aggggatagc
       61 cgcggacgat ctccccgttc gaataacgac tttccgcacc cgcctgcagg cgccggtcga
      121 gagagcggat cgcctgctcc atcgggtcga aaggatcctt cttgctcgcg agcatcccgt
      181 acagaacgac gtcgcgagcc gcctcgggga gttcggctcc tgcggagacg tccgagaagg
      241 tcccgtcggc gaagaccctc tgcacggcca tctgattgag cgtcagggtt ccggtcttgt
      301 cgacgcacag cacggtcgcg gccccgaggc tctcgatggc cggaagctgc cgggtgagga
      361 cgcgccggcg cgagatccgc cacgctccca gcgcgaagaa gatcgtcagg atgacgggga
      421 attcctccgg caggatggcc atggcgaggg cgagacccga aagcaggccc tggagccagt
      481 tgccgcgggc cagcccgtag aggacgacga cccccgcgca cagcaacagg ccgaccaggg
      541 cgatccggcg cacggcccgc ccgatctcct cctgcagccg ggtctttccg ggagcgatcg
      601 cgccgagcga tcggccgatc ttcccgatct cggtcgaggc gccggtcgcc tcgacgcgcg
      661 cgagggcgtc tcccgacacg acgagcgttg ccgagaagac gcgggccggc gcgtgcccgc
      721 tcgccacggc cttggcaacg ggcaccgact cgcccgtcag aagggattcg tccgccgaga
      781 ggttcgactc ttccagaacc gtcgcgtcgg cggggacgcg atctccttcc gagagaacga
      841 tgaggtcccc cgggacgact tcgcggcccg gaatcctcct gcggactccg tcgcggatca
      901 cgagtgcccg cgggctcgag agatcccgga gagcctgaag ggcgtgctcg gttcgacggt
      961 tctggaacac ggagatcgcg atgaccacga gaaccgagaa gaggagaacg atcgcttcgc
     1021 gccgggagcc caggagaagg tagatcgcgc cggccgccag cagcatcagg atcatgggct
     1081 cccgcacgac ctcgaacgcc gttcgaaaga tgctcggacg cgcggccgag gggagctcgt
     1141 tgtagccgac ctgctcgagg atcgccgcgg cgtcgcgctc ggtcagcccg agctcggcgg
     1201 ggtccggagt catgctcgca ggatgccgcc gccgacgcgg gtcaggagcg tcgacgcgcg
     1261 ggtaccacga gcatcgggat cggcgagcgg acgagcatct cctgcaccgt gcttccgggg
     1321 aggaacgccg ccacacggcc gcgtcctcga ttccccatca cgatgaggtc gtacccccgg
     1381 cggctctcgc ggaggagctc ccgggccggg tcgccccgaa ggagccggat cgactcggtc
     1441 tttccccggc ccaggaccct ggagaaccgt gactcgtagt gttctgcaat cgcgcccgcg
     1501 agcgcgcccg cggcaagccg cagcagatct ccccgctcct cgagaaccgc ggcccgcacg
     1561 tccgagagcg gtcggacgtg ggcgaccgtg agctcgcccc cggtgcgcgt gacgagagct
     1621 cgtgccgcct tgagcgcggc gagcgactcc ggcgaaaagt cgagcgcgac gagagctcga
     1681 aacggggcag ccaccgcgtg gaattctatc cggacgggac ggagctggcg gcggcgtccg
     1741 ccgcgacaca accaaccatc cgggcggaaa tcggcgtacg gaattccacg agcgtcgtcg
     1801 ttccgtccgc cgagttgatt ccgattgcct gctcggcgcc ttcctccgtg agctcttccc
     1861 acacggccat cggctggggg acggggtgct cgacgttgcc gccggccgga ccgccgaggg
     1921 agatcgtgat cgtagtcccg agcgggtctg cgacgattcc ctcgagcggc aggtcccgcg
     1981 cctcgacctg cgctccgatg tgaggaccga gcagccacac ggtcgtgggc gagccggggt
     2041 ggcgccgcgt gaaggcttcg aagaacggga accattcggc atgagggatg agccgcgtac
     2101 gcatttttcg ctcctcgatc gaaggttgca ccaacttcgg gaaaaattgc tgatgcaaac
     2161 gcttctatcc tcgtcaccgt ggtcgtccga agatcgccgc gtcgaaaggg agccctcatg
     2221 gaagcaggta tcttgaatct cgcgtctccg ggccggtcgc tcgatgtcgc cccgaaaggg
     2281 gccgtcgtcc ccccggagcg ctacgaaagc gacgtcgtcc tgaaaaacgg ctcgacgctc
     2341 catctgcgtc cggtccgccc ggaggacgcc ccgggtctcc tcgagttctt ccggggactt
     2401 tcccccgaga gtctttacct ccgcttcctc ggcgtatcgc ctttcgatct cgcccgcgcc
     2461 gaaaacctcg cgcgcgcgga ctacgagagc cagttcgggc tcgtcgggga gacccggcag
     2521 ggaatcgtcg cgctcgggca tttccaccgc gacctggaga ttccggaacg cgcggaggtt
     2581 gctttcgcag tcgccgacgc cctgcagggg caggggatcg gaacgagcct cctcgaccgg
     2641 ctcgcgcgcg tcgcccgcga aatggggatc aggaccttcg tcgccgaggt gctcccttac
     2701 aaccggaaga tgatcgaggt cttcgtcgac tcgggcttcg ccgtccgcca gaggaccgag
     2761 gacggggtgg tgaggttcga aatctcgctc gcttcgaccg cggcgtcaac cgagagagcc
     2821 gcggagcgcg cccgccacgc cgcgaccgcc tcgatgcagg ccttcttcga gccgcgcagc
     2881 gttgccgtcg tcggggccga tcgcacgcgc ggacgcatcg ggtcggagat cctccacaac
     2941 ctccgcgcct ccggtttccg cggcgggatc ttcccggtca atccccacgc cgacacgatc
     3001 cagacgctcc gttcctaccc gcgggtgacc gacatcccgg aggaggtgga tctcgcggtg
     3061 atcgtcgttc cggccacgag agtcgacgcg gtggtggacg actgcctcgc gaaaggggtc
     3121 aaggccctcg tcgtgatcag cgccggtttc ggcgagacgg gcgaggccgg gcgcgcgcgc
     3181 gaagcagcgc tcgtcgagaa gctccgatcg gcgggcgtcc ggatgatcgg acccaactgc
     3241 ctcgggatcc tcaacacgga tccgaaggtg cggttgaacg cgacgttctc gccggtctac
     3301 ccgcccgagg gccgagtcgg catgctctcc cagagcggag cgctggggct cgcgatcctc
     3361 gactacgcgc ggcagttgaa cctcggcatc tcgacgttcg tctcggtggg caacaagccg
     3421 gacgtgtccg gcaatgacct catacagtac tgggagaacg acccgcggac cgacgtcatc
     3481 ctcctctacc tcgagagctt cggaaacccg cgcaacttcg gaccgatcgc ccggcgggtc
     3541 tcccgctcga agccgatcgt cgtcgtcaag gccgggcggt ctcgcgccgg cgcgcgtgcg
     3601 gcgtcctcgc acaccggcgc gctcgcggag agcgacgccg tcgtcggggc gcttctgcgg
     3661 cagtcgggcg tgatccggac gcggacactc gaggagctgt tcgacgtcgc caccctcctt
     3721 tcgcaccagc ctcttccggc gggaaagcgc gtggcgatcc tgaccaatgc cggagggccc
     3781 gggatcctcg ccgcggacgc gtgcgaggcg ctcggcctct ctctcgcgtc tctctcgaaa
     3841 gagactgttg cggcgttgcg cgagttcctt ccggccgaag cgagcgtggg caatccggtg
     3901 gacctccttg cgtccgcgtc cgccgaggac tcccgtcgcg ctcttcgtct cgcgctggcc
     3961 gaccgcggag tcgacagcct cctcgtcatc ttcatcccgc cgctcgtcac cgacgccgac
     4021 tccgtcgccg cagcggtggt ggcggaagcc cgggagtcgt cgaagaccgt ggtcgccacg
     4081 ttcatgggtg ccaagggtgc tcccccgggg ctcgccccga ttccctgcta tccattcccg
     4141 gagtcggccg tcgccgctct ctcgcgggcc accgaatacg ccgagtggcg tcggagaccg
     4201 gcggggacgg ttcgcgaact cgccgacgta cgccccgacg acgcccgcga tttcgtgacg
     4261 cgcgctctcg aacgcggtga gggctggttg acgcccgcgg aagcgcggtc ggtgctgacc
     4321 gcgttcggga tcccggtcgc gagatcgcgc atcgctcccg gctgggacga ggtccgtcgc
     4381 gcggcgcggg agatcggtta cccggtcgct ctgaaggcga tcgggcccac gctcctccac
     4441 aagagcgacg tcggcggtgt gaagctcgac gtcgcgaact cggcggctct ccaacgcgcc
     4501 ttccgggacc tggaaacccg gttgggcgat cgcgtcacga gctacctcgt gcaggagatg
     4561 gtcccgggcg gagtcgaggt catcgtcggg gccgtcttcg accggacgtt tggccctctc
     4621 cttctctacg ggagcggcgg gacactcgtc gagctcctgg gcgacacgtc ctttcgcatc
     4681 gcgccgctct ccgacgcgga tgcagccgac atgttgaacg aggtgaaggg gaccgcgcgg
     4741 ctgcgcggat tccgcggcgc gccgccagcc gacgagaagg ctctcaccga tgtcctgctc
     4801 cgcgtctcgg cgctcctgga agcgtgcccg gagatccagg aaatggatct caaccccgtg
     4861 aaggttttcg agtcgggcgc gaaagtcgtc gatttccgga tccgggtcgg gacgccgccg
     4921 gcgccgacgc gcgcgcgacg gatcagctac tgaagggaga aggaatgaca gcggtcgaga
     4981 ccgctcgagg aatcgaatcg ctcgacgagt ccggcaaacg cgccaccatg aaggcgttcg
     5041 tgatgcgcga gatcgggaaa gtcggcctca tggagaagcc gatcccgccg gtcggcccga
     5101 acgacgcgct cgtcaggacg acggccgcgc tcatctgcac ctccgacgtc cacacggtcg
     5161 gcggagcgat cgggccgcgg caggaccgga cgctcggaca cgaagcggtg ggggtgatcg
     5221 agaaactggg ctccgccgtc tcggggctcg acgtgggaca gcgagtgacc gtcggcgcga
     5281 tcactccgtg ctggcgctgc gagaactgcc tgcgcggctt tccgtcgcag tgtcacgaag
     5341 ccctcggggg ctggaagttc gcgaacgtca aggacgggaa cctcgcggag ttcttccacg
     5401 tcaacgacgc ggaagcgaac gtgacgccga tccccgacga agttcccgac gagctcgccg
     5461 tctacacgtg cgacatgatg tccaccggct tcatgggcgc ggagcgcgcc gcgatcccga
     5521 tcggggggac ggtcgcggtg ttcggcgccg gcccggtggg tctgatggca atcgccggcg
     5581 ccaggctcct cggagccggg ctcgtcatcg cggtcgagcg ccccggaaag cgcgcggact
     5641 tcgcgcgcta ttacggcgca gaccacgtcg tcgacttcgg tcggctcgat ccggcccggg
     5701 agatcgggaa gctgaccggc ggaaaaggcg tcgactccgc gatcgaagcg gtcggcagcg
     5761 ccgagagctt tgccagctgc gtcaaggcga cgcggccggg cgggaccatc tcgaacgtcg
     5821 gctaccacgg agagggcgac gtcgttccga tcccgcggct cgcgtggggt gtcggaatgg
     5881 cggacaagac gatccggtcg ggtctctgtc ccggcgggcg cgagcggatg gagaggctgc
     5941 tgcggctcct tcagaagaag cgcgtcgatc cgggcccgct gacgacgcac cgcttccgct
     6001 tcgacgaagt cgagcgcgcg ttcgagatga tgaggacgaa ggaagacggg atcatcaagc
     6061 cgctgatcct cttctgagcg caaccgtccg ctagccgagg accgggaaag gcgcgatttc
     6121 tccgcgaagc gcggcgagct cgtccttcgt gagccattcg tcgagaggag tcccgagaag
     6181 gctcgcaccc gcctcgagcg cgtgcgccca gctcgagcga ttgacgaaga gcatcccgcg
     6241 cgtatcgagc gtgccgccgc ggtttgagaa tccgagcccc accggacgcg gcccgatgac
     6301 ctccggagcg agcacgccaa gcaggacctc cgggcgagtg tgcgtcacga agacgcgggc
     6361 ttcgaccgat ccgggccaga gcgcggccac gcgatcggcg ggaacgacgt gtctcgcctc
     6421 gccgtcggtc cgaggccgcc ggaagcgtcc cggctcggaa acatagacga ccgcgtgcgg
     6481 gacgcccttg cccgccagcc ggaacgacgc cttgaggact tccgcgagct ggtaggcgcc
     6541 gacggcggtc agcgagagtc gcgcgcgctc cggctcgaaa cccgcccagt cgagcgcgat
     6601 cgcgccttca ttcgccaacc tctcggcctc ttcgtgctcg aatgcgtccg cgacactctc
     6661 ggccttcggc acgacgagcg tccagatctc gccgcgcgtc gagaacacgg agaggatcgc
     6721 cgcggccgcc gaattcgcgt cggcggggaa gacgacccgc gagacgtccg aggactctcc
     6781 gagcagagcc tccgcgagcg acgggtcctg gtgcgaccgc tcgttcttcg cgttctccca
     6841 cgcgtgcgac gcgaggacga tcgggaccga gagccagccc ggaggacggc cggcgtgccg
     6901 cagctcctcg gagaagacga tctcctgccg aagcgcgccg tgcatcttca tcccgaacgc
     6961 ctcgtacgtg acgacgatgt tgatgcctcc cttgtttccg agtgcggcgc agacgatggc
     7021 ctcctcgttc agcgcggtga tcaccgcacc ttccaccgct tccggcaggc cgtgctccgg
     7081 cgccgtcacc cggaacttca gggcatcgag cgtcgcgagc atccggttcg agcgcatttc
     7141 gtccgggttg ccgacgcgcg ggcgcagccc cgggttcgcg cgcacgaccg cgaggaacgt
     7201 ctcgtcgacg gcggtcatcg gggacgatct cgtccagcgc gccgggtcgc gccggttcgg
     7261 cgagatcggc cgcgcgttcc cggccggcgg caccagggcc cggacgtcgc gccgcgcgag
     7321 cggatggtcc ccctcgcgca cgcgcccgga ttctccgtgc gcggcgaact tctcgagcgc
     7381 cggccgaagc tcctccggcg gcacccagag cttgcgcgcg ctgtcgcgga agtgccgcgc
     7441 ggcggtcgcg tcgcggctcg ggttcgcgcc gagcggcagg ttgtgcgcga gcagcttgcc
     7501 cgcgccccag aacccggcgc ccttcggtgc gaccgcgatg ccgtagggca gggggacggg
     7561 atactcgatc cggccagcgt gtacttcttc ggagagtttc tccagccgcc gttccatctc
     7621 gaagatcgcc cacacgaacg ccgccgggtc gcggccgtcg aagatgatcg ggtggaagcc
     7681 gttcagccgc aggtgctgcc ggaaccagga gacgcccccc gactgggcca tcgtcgtgcg
     7741 ctggtcgatt cggcggccgt tggagatcat gatcggtgcg acgaggccgc tgtcttccgc
     7801 gcgccaccaa cgcggcgccc agtcgctccc tctctgctct tcgaacgctc cgtcggagag
     7861 gaagaccacg agccgctcgc ccggcagcgg catgtggatg tactggagct cggcgaagcc
     7921 aaggtatccg ccctcgaacg tcccccccgc ggtgttcggg ttgacgtggc ttccgagcgg
     7981 cgactcggga acgccgccgg ggccgaggcg gtacgagtag aagtcctcgc agaagcgcga
     8041 gagcccttcg tccgagacgt cgtagcgcgc ggcgtgcggg ggcgtcgtgt ttccgacgag
     8101 caggttgacc gagtcgagcg cggacacggt gtgcccctgt ccgacgagcc acgaccgcgt
     8161 gaacccggag atcgcgttcg ccgcgaggta gcccgcgtaa cccggcacca tgttgagcga
     8221 gccgcccatg tggccttccg gcctctcttt gaactcggaa ggcgcgaggt cggtgccgtc
     8281 gaggtgcacg cggcgcgcgt acgtctggtg cgcgacgagc cacatgccgg cgcgcgcgag
     8341 ccggtcgagc gcggagagga ggtcgaagag cgggactccg tcgccgcgga tcccgcgccg
     8401 cgcgagatct tcggcgagac cggcgatctc gacctgcgtc tcgggaaggt ggcggatcgg
     8461 gccgccgccc tccgcccagg ctcgcagggc cgggtcgctc tcgcgcagct ccgcggcgcg
     8521 gcgggcgaga gacttgcggg gggcggacgc gctcattgca ggagcaccgc agcgtcgcga
     8581 gcgatcaccg cctcctcgtc cgtcggggtg acccacacct cgatccgcga gccctccgcg
     8641 ctgatgcgcc cgacttctcc gcggatccgt tcgttgcgcg cctggtcgag cgagatcccg
     8701 agctcttcga ggccgtccag cattgccgag cgaaccgtcg cggcgtgctc gccgatcccc
     8761 ccgccgaaaa gaatcgcatc tgcgccgccg agcgcggcga ggtaggcgcc caggtacttg
     8821 cgcgcgcgat acgcgaagat ctcgatcgcg agccgcgcgc gggagttgcc ttcatcggcg
     8881 gcctgctcga tctccctcac gtcgcgtgaa actccggaga gcccgagcaa cccggaccgg
     8941 tcgttcaact gcgcgaggac ttccgcgccg gtcatccctt cgcgctccgc gatgaacggg
     9001 acgagggacg catccaggtc gccggagcgc gttcccatga cgagaccctc gagcggcgtg
     9061 aagcccatcg acgtgtcgat cgagcggccg tcgcgcagcg cgcacgcgga ggcgccgttg
     9121 ccgaggtgaa aggcgatcag acgggacggt tcgatccgcc ggccggtctc gccgaaataa
     9181 cggtcgcaca tggaacggta ggagaggccg tggaagccga accggcggat tccgagccgt
     9241 tgcgccagct cgagagtcag cgcgtagcgg aacgcccgct ccggcaacgt ccgatgaaac
     9301 gccgtgtcga agacggcgac ctggggaacc gggggcgtcg cggtccggcg gcactcgcgg
     9361 atgacggcga gcgccggcgc gttgtgcagg ggcgcgaggt ccgtcgcctc ttcgatggcg
     9421 gccagggtct cgtcgtcgac gcgcgccggt tcccggaagc gttcgccgcc gtgcacgacc
     9481 cggtgcgcgt aggcctcgaa gcggtcttcg ccgcgacggg agagatcgtc cagcacacgt
     9541 cgcaccgcca tcgcgtggtc gggcggagcc tcgcctcctt cgccgatccg ctcgatctct
     9601 ccacgcgccg tttcggcggg cgcgtcgccg ctcgcgagct cgaaaagccg gtacttgagc
     9661 gaggagctcc cggcgttgaa gacgaggagt ctcaccggct cctcttatca tgcggccgga
     9721 agaccggggc tacgggaggc gggtcggcgg cggcccgtcg cgccgctcga cttgaatccg
     9781 gtggtagtcg gcgagcgctt ttcggacgat cttctgcagg ttcctcatcc gccgggccga
     9841 tcgccgtcgt tcgagctggt cgagccgcgc ccggcgtctc tggacggtct cgcgccacag
     9901 gagatccacg gcgcgtcgga ggcgcgcgga caggagctcg gctgtcaggt ccagcgcgtc
     9961 gctcacgggt cgattctctc ccggtttttg ccaaaaaggg tgctgattcg tttgcatcaa
    10021 gtgg
//
LOCUS       DASWAV010000100         3890 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U2665
            k141_365568, whole genome shotgun sequence.
ACCESSION   DASWAV010000100 DASWAV010000000
VERSION     DASWAV010000100.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960316
            Sequence Read Archive: SRR10260007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 3890)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3890)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:47:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,929
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3890
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_365568"
                     /isolate="SMAG_U2665"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.36444 N 132.31889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..964)
                     /locus_tag="VF958_02135"
     CDS             complement(<1..964)
                     /locus_tag="VF958_02135"
                     /inference="COORDINATES: protein motif:HMM:NF014081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX9147939.1"
                     /translation="MRRLLTALLLLVSTRAGAAEAPRITLALVGGRIVDGYEGRPIEN
                     GVILVAGERIAALGPRSEVVVPPGVPTIDTRGMTVLPGLADMHVHLMILGHGDYEHWD
                     TTYRPRFRGEIMPAAARQLVTSGVTFARELGAPLDDVLDVKRRIDRGEIPGPRLFVSG
                     PFIQHRPYFEYEKEYRWGVNGAADARAKVQKLVDAGVDVIKLIDQDQMTEEEVKAVVE
                     TAHKRGKPVVAHAHREEEIRLGLKYGIDSFEHTGLATEPGYPEDILQGIRKRNQTLFW
                     CPTIAPLFLSEYTADSFPERLNDPRWQEGLAPDIVSDIRKSLENL"
     gene            1010..2347
                     /locus_tag="VF958_02140"
     CDS             1010..2347
                     /locus_tag="VF958_02140"
                     /inference="COORDINATES: protein motif:HMM:NF023128.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX9147940.1"
                     /translation="MTSARVEVTPSGELRVLERAPIVERASWALYDFANTIFSMNIAT
                     LYFAVWIVTERGASATMFSIATSASSAVVLFAAPYLGARSDLSRRRKPWVVGLTLACV
                     AATAFLAPLAGASLAPATSLLLLLATFAVADVAYQLALPFYNAMMPELVPESEHGRLS
                     GVGTAVGYAGSIVGVLAAAPFVSGQWSIGGPPGRPAAFLPTAALFLLFSLPLFFFCHD
                     HIPRPRSERRAVRFREIAAKIAEAFGQTRKYPGLRRFLFASYLYQDALGTAIAFMAIY
                     AVKVLGLARGEETRLFVALTVPAVIGSFLAGLVSDRLGPRRTLLIVLWGWVAGLAAIA
                     LAPNLSAFWAGGVLLGFVFGGIWSVERPLLLTLVPDAEGGRFFGLLVLSARAAAIAGP
                     LLWAFVADHVFAGFGAGVGYRAAVASLILFMLGAIVLLRGVPDARLHSRRASA"
     gene            2373..3377
                     /locus_tag="VF958_02145"
     CDS             2373..3377
                     /locus_tag="VF958_02145"
                     /inference="COORDINATES: protein motif:HMM:NF040257.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate reductase"
                     /protein_id="HEX9147941.1"
                     /translation="MNEVIARVALVGLGPIGIEVGRAVQGRSGIEVLGAADPATDKAG
                     RTLGEILGGDGPFPVVASARDLYHQTSARRGRRDVVLLCTGSRLESVAPQIEEAVEAG
                     FHVVSTCEELSLPGFRHADLAGRIDAKAKQHGVAVLGTGVNPGLVMDRLALAAAGACV
                     TVEKLKVTRVVDAAKRRGPLRAKVGAGLTKEEFEAGVAARKLGHVGLSESAAMIAAGI
                     GQPIGEVSETIEPVLASVETDGIAPGRVLGLHQIARVRAAGGVKVELDLTMAVGAKAF
                     DRIEIQGDPPVNLTVEGGFHGDRATVGCVVNAIPFVVASPPGIQTVVTLPLFGLLPQH
                     "
     gene            3380..3760
                     /locus_tag="VF958_02150"
     CDS             3380..3760
                     /locus_tag="VF958_02150"
                     /inference="COORDINATES: protein motif:HMM:NF025887.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4440 domain-containing protein"
                     /protein_id="HEX9147942.1"
                     /translation="MSSEPRTPEDVHRLWSEYFVAGDLEGLVSLYEPDAAFLAQSGAF
                     VTGRRAIREVLAGFIAVRRGFEIKIGRAIQTGDIALLVSSWKLQGEQRGEPYETSGQT
                     ADVVRRQPDGSWRLVIDSPYGEGA"
     gene            3757..>3890
                     /locus_tag="VF958_02155"
     CDS             3757..>3890
                     /locus_tag="VF958_02155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014061824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HEX9147943.1"
                     /translation="MTATPDPAGRILLTGATGYVGGRLLEELVRAGRRVRCVARRPER
                     L"
BASE COUNT          517 a         1338 c         1351 g          684 t
ORIGIN      
        1 gcaggttctc gagcgacttg cggatgtcgg agacgatgtc gggcgcgaga ccttcctgcc
       61 agcggggatc gttcaggcgc tccggaaagg aatccgcggt gtactcggag agaaagaggg
      121 gtgcgatcgt cgggcaccag aagagcgtct ggtttcgctt acggatcccc tggaggatgt
      181 cctccgggta gccgggctcg gtcgcgagac cggtgtgctc gaaactgtcg attccgtact
      241 tcaaaccgag ccggatctct tcttcgcggt gcgcgtgggc gacgacgggc ttgcccctct
      301 tgtgggcggt ctcgacgacc gccttcacct cttcttcggt catctggtcc tggtcgatca
      361 gcttgatgac gtcgacgccg gcgtccacga gcttctggac tttcgcgcgg gcgtccgcgg
      421 cgccgttgac cccccaccga tactccttct cgtactcgaa atacggccgg tgctggatga
      481 aaggacccga gacgaagagc cgcggcccgg ggatctcgcc cctgtcgatg cgccgcttga
      541 cgtcgaggac gtcgtcgagg ggcgcgccga gctcccgcgc gaaagtgacc ccgctcgtca
      601 cgagctggcg cgcggcggcc ggcatgatct ctccccggaa cctcggccgg tacgtcgtgt
      661 cccagtgctc gtagtcgccg tgcccgagga tcatcaggtg cacgtgcatg tcggcaagcc
      721 ccggcaggac cgtcatcccg cgcgtgtcga tcgtgggaac gcccggcggg acgacgacct
      781 ccgagcgcgg gccgagcgcg gcgattcgct ccccggcaac gaggatgact ccattctcga
      841 tcgggcgccc ctcgtagccg tcgacgatgc gcccgcccac gagcgcgagg gtgatccggg
      901 gggcctcggc cgcacccgcg cgagtcgaaa cgagtagcag aagggccgtg agaagtcgac
      961 gcatccttgt cctccggctg ctggctgaca gcgactatcg tattccgtcg tgacgagcgc
     1021 gcgagtggag gtcacccctt cgggcgagct ccgcgtgctc gagcgcgcgc cgatcgtgga
     1081 gcgcgccagc tgggcgctct acgacttcgc caacaccatc ttctcgatga acatcgccac
     1141 gctctatttc gccgtctgga tcgtaacgga gcgcggcgcc tcggcgacca tgttctcgat
     1201 cgcgacgagc gcttcgtcgg ccgtcgtcct cttcgcggcg ccctatctcg gcgcccgttc
     1261 ggacctctcc cgccgccgga aaccgtgggt ggtcggactg acgctcgcgt gcgtggcggc
     1321 gacggccttt ctcgccccgc tggcaggtgc ttcgctcgcg ccggcgacct ccctcctcct
     1381 cctgctcgcg accttcgcgg tcgccgacgt cgcctaccag ctcgcgctcc cgttctacaa
     1441 cgcgatgatg ccggagctcg tgccggagtc ggagcacggc cggctctcgg gcgtgggcac
     1501 ggccgtcggg tacgcgggct ccatagtggg tgtgctcgcc gctgcgccgt tcgtctccgg
     1561 gcagtggagc atcggaggcc ctccgggacg gcccgccgcg ttcctcccca cagcggcgct
     1621 cttcctgctc ttttctctcc ccctgttctt cttctgtcac gaccacattc ctcgaccccg
     1681 gtccgagcgg cgcgccgtga gattccgcga gatcgccgcg aagatcgcgg aagcgttcgg
     1741 acaaacgagg aagtacccgg gcctgcggcg ctttctcttc gcgtcgtacc tctaccagga
     1801 cgcgctcggc accgcgatcg cgttcatggc gatctacgcg gtcaaggtgc tcggcctcgc
     1861 ccgcggagag gagacccgtc tcttcgtcgc gctgacggtg cccgcggtga tcggctcgtt
     1921 cctcgcgggt ctcgtgagcg atcggctcgg tcctcggcgc acactgctga tcgtgctgtg
     1981 ggggtgggtc gccgggctgg cggcgatcgc gctcgcgccg aacctctcgg cattctgggc
     2041 cggaggcgtc ctactcggct tcgtgttcgg agggatctgg tcggtcgagc ggcctctcct
     2101 cctgacgctc gtcccggacg cggagggggg gcggttcttc ggccttctcg tcctctccgc
     2161 gcgagcggcg gcgatcgcgg ggcctctcct ctgggccttc gtcgccgacc acgtcttcgc
     2221 ggggttcggg gcaggcgtgg gttatcgcgc cgcggtcgcg tcgctcatcc tcttcatgct
     2281 cggcgccatc gtccttctcc gcggagtgcc ggacgcgcga ttgcattcgc ggcgggcgag
     2341 cgcataaggt tcgcatttcg ccgggagggg gaatgaacga ggtgatcgcg cgcgtggcgc
     2401 tcgtggggct cggtcctatc ggaatcgagg tcggccgcgc agttcaggga cgttccggca
     2461 tcgaggtcct cggagcggcg gacccggcaa cggacaaggc ggggcggacg ctcggcgaaa
     2521 tcctgggcgg cgacggcccg ttcccggtag tcgcgtcggc gcgcgatctg taccaccaga
     2581 cctcggcccg gcgcggccgg cgcgacgtcg ttctcctctg caccggctca cgtctcgagt
     2641 cggtcgcgcc tcagatcgaa gaggcggtcg aggccggttt ccacgtcgtg tccacgtgcg
     2701 aggagttgtc gctgccgggc ttccggcacg ccgatctggc gggaagaatc gacgcaaagg
     2761 cgaagcagca cggggtcgcc gtgctcggga cgggcgtcaa tcccgggctc gtcatggacc
     2821 gcctcgcgct cgccgcggca ggcgcgtgcg tcaccgtcga gaagctgaag gtcacgcgag
     2881 tcgttgacgc ggcgaagcgc cgtggcccgc tccgcgcgaa ggtcggcgcc ggcctcacga
     2941 aggaggaatt cgaagcgggc gttgccgccc ggaagctcgg gcacgtgggt ctctccgaat
     3001 ccgccgcgat gatcgcagcg ggaatcggtc agccgatcgg ggaggtctcg gaaacgatcg
     3061 agcccgtcct cgcgtccgtc gagacggatg gcatcgcgcc gggacgcgtg ctgggcctcc
     3121 accagatcgc gcgggtgcgc gccgcgggag gggtgaaggt cgagctcgac ctcacgatgg
     3181 ccgtcggcgc gaaggcgttc gaccggatcg agatccaggg cgatcctccc gtgaacctga
     3241 cggtcgaggg gggctttcac ggcgaccggg ccacggtcgg atgcgtcgtc aacgcgatcc
     3301 cgttcgtcgt cgcctcgccg cccggcattc agacggtcgt cacgctgccc ctcttcggtc
     3361 tcctgccgca gcactgatca tgtcctccga gccgcgaact cccgaagacg tccaccgcct
     3421 ctggagcgag tacttcgtcg caggcgacct ggaaggcctc gtttccctct acgagcccga
     3481 cgccgcgttc ctcgcgcagt cgggggcctt cgtgacgggt cggcgagcga ttcgagaggt
     3541 gctcgccgga ttcattgccg tccggcgcgg cttcgagatc aagatcggac gcgcgatcca
     3601 gaccggcgat atcgcgctcc tggtctcgtc ctggaagctc cagggcgagc agcgcggcga
     3661 accgtatgag acgagcggcc agaccgccga cgtcgtgcgg cgacagcccg acggctcctg
     3721 gcggctcgtg atcgacagcc cgtacggcga aggcgcctga ccgcgactcc cgatcctgcc
     3781 ggccggattc tcctgacggg cgcgaccggc tacgtcggcg gacgtctgct cgaagagctc
     3841 gttcgcgcgg gacgtcgcgt gcgttgcgtc gcgcgccgac ccgaacgtct
//
LOCUS       DASWAV010000101         2739 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U2665
            k141_165539, whole genome shotgun sequence.
ACCESSION   DASWAV010000101 DASWAV010000000
VERSION     DASWAV010000101.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960316
            Sequence Read Archive: SRR10260007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2739)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2739)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:47:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,929
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2739
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_165539"
                     /isolate="SMAG_U2665"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.36444 N 132.31889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..246
                     /locus_tag="VF958_02160"
     CDS             <1..246
                     /locus_tag="VF958_02160"
                     /inference="COORDINATES: protein motif:HMM:NF016027.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016758 - hexosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX9147944.1"
                     /translation="VPFGSATHGHHTENARAFERAGAAISIEEGTLTGETLARTVREL
                     IGSRPRLVAMGERARAFARPDAAKILADLLFEAEGTR"
     gene            243..1649
                     /gene="murC"
                     /locus_tag="VF958_02165"
     CDS             243..1649
                     /gene="murC"
                     /locus_tag="VF958_02165"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010943693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008763 - UDP-N-acetylmuramate-L-alanine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HEX9147945.1"
                     /translation="MRFLKLRRIHFVGAGGVGMSGLAEILLLSTPLEISGCDRESGEN
                     TERLAALGAKIALGHDPAHLANVDVLVISSAVDESNPEVSAARERGIPVIRRAQMLGQ
                     IMRLKQGIAVAGTHGKTTTTSLTGMVLTEAGLDPTIVVGGRVRILGTNARLGRGDFLV
                     AEADEYDRSFLELTPDVAVITNVEADHLDCYRDLDEIVDAFVQFANRVPFYGAVVACL
                     DDLGVGAVLPRVEKRVVTYGESPQAHIRARDVRLEASGTRFEAWDSEQGRLGEVSLPL
                     PGRHNVANALAAIAVGRELLIPFETIARALAGFTGVVRRFERKGERGGVLVVDDYAHH
                     PTEISATLAAARQVYPDRRLVALFQPHLFSRTRDFAVEFGRALLGADVAVVTDVYPSR
                     EKPLPGVTGQLVVSAAREAGHRNLVYLSDKKSVVDRLERLLRPGDLLLTMGAGDVVRF
                     GEGYLAGEGTRRVADAGK"
     gene            1636..2445
                     /locus_tag="VF958_02170"
     CDS             1636..2445
                     /locus_tag="VF958_02170"
                     /inference="COORDINATES: protein motif:HMM:NF020067.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsQ-type POTRA domain-containing protein"
                     /protein_id="HEX9147946.1"
                     /translation="MPESRPDPAFVAVTATEKKSFLRRGAIAPRRRKRGRVVRLALAV
                     IVVAAAAVGLSRFFGGSQFALQRIEVSGNRRARTEEILRAVEPWRSSNLVGLDLSAIA
                     RAVRTHPWIERVTLSKRLPDGLSIRVTEKKAVALYRDASGLSWLSSDGHPIASYDPRA
                     DSTEYVLVSGDLRALPDAVALLDDIRSAPAEYFSALSEISALPDGGFGIMDTIFRKPV
                     RVLRRDAPAKIRALLEARSFIESRGWEARAIDLRFADRIVLVGAYGAGNSL"
     gene            2522..>2739
                     /locus_tag="VF958_02175"
     CDS             2522..>2739
                     /locus_tag="VF958_02175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HEX9147947.1"
                     /translation="MPGVLMAKPENFLVSCDIGSSKICVLIGEQNAHSALDVIGKGAA
                     PNRGTRKGNIINVDATIEAIKRAAEEAE"
BASE COUNT          420 a          908 c          945 g          466 t
ORIGIN      
        1 gtccccttcg gatccgcgac gcacggtcat catacggaaa atgcccgggc gttcgagcgc
       61 gccggggcgg ccatctcgat cgaagaggga acgctgacgg gagaaacgct cgcccggacc
      121 gtccgggagc tcatcggcag ccggccgcgg ctcgtcgcga tgggagagag agcccgcgcc
      181 ttcgcgcggc cggacgcggc gaagatcctc gcggacctcc tgttcgaggc ggaggggacg
      241 agatgaggtt cctgaagctc cggaggattc acttcgtcgg cgcgggcggc gtcgggatga
      301 gcgggctcgc cgagatcctg cttctttcca cgcctctcga gatctccgga tgcgaccgcg
      361 agtccgggga gaacaccgaa cggctggcgg cgctcggcgc gaagatcgcg ctcggccacg
      421 accccgccca cctcgcgaac gtggacgtcc tcgtcatctc gtcggccgtg gacgaatcga
      481 accccgaggt ttcggcggcg cgcgagcggg ggatcccggt catccggcgg gcccagatgc
      541 tcggccagat catgcggttg aagcagggga tcgccgtggc cgggacgcac ggcaagacca
      601 cgacgacctc tctcaccggg atggtcctga ccgaggcggg gctcgatccg acgatcgtgg
      661 tcggcggccg cgtgcgcatc ctcgggacga acgcgaggct cggccgcggc gacttcctcg
      721 tcgccgaggc ggacgagtac gaccggtcct tcctcgagct cacccccgac gtcgcagtga
      781 tcacgaacgt cgaggccgac cacctcgact gctatcggga cctggacgag atcgtggacg
      841 ccttcgttca gttcgccaac cgcgtcccgt tctacggcgc agtggtggcg tgcctggacg
      901 atctcggagt cggggcggtc ctcccgcgcg tcgagaagcg ggtcgtcacc tatggggagt
      961 cgccgcaggc gcatatccgc gcccgcgatg tccggctgga agcgtccggc acgcgattcg
     1021 aggcctggga ctcggagcag gggcgcctgg gagaggtctc gctgcccctt cccggccggc
     1081 acaacgtggc caacgcgctc gcggcgatcg cggtggggag ggagctcctg atcccgttcg
     1141 aaacgatcgc tcgcgctctc gccggattca cgggagtcgt gcggcgcttc gagagaaaag
     1201 gggagcgggg cggggttctc gtcgtcgacg actacgcgca ccatccgacg gagatctcgg
     1261 cgacgctggc tgccgcgcga caggtctatc cagaccgtcg cctggtcgcc ctctttcagc
     1321 cacacctctt ctcgcgcacg cgcgacttcg cggtcgagtt cggtcgcgct ctgctcggcg
     1381 ccgacgtcgc cgtcgttacc gatgtgtacc cgtcgcggga gaagcccctg cccggagtca
     1441 ccgggcagct cgtcgtcagc gccgcccggg aagcgggaca ccggaatctg gtgtatctct
     1501 ctgacaagaa gagtgttgtc gaccgcctcg agcggctcct ccggcccggc gacctgctgc
     1561 tgacgatggg ggcgggcgac gtggtgcggt tcggcgaggg gtatctggca ggcgaaggaa
     1621 cgcggagggt cgccgatgcc ggaaagtagg ccggacccgg cgttcgtcgc cgtgaccgcg
     1681 acggagaaga agtcgttcct ccggcgaggt gcgatcgcgc cgcgccgccg gaaacgcgga
     1741 cgggtcgtcc gcctcgcgct cgccgtgatt gtcgttgcgg ccgccgcggt cgggctctct
     1801 cgtttcttcg gcggatccca gttcgctctc cagcggatcg aggtttcggg gaaccgacgc
     1861 gcgcgcacgg aagagattct ccgagcggtc gagccatggc gctcctcgaa cctcgtcgga
     1921 ctcgacctct ccgcgatcgc ccgtgcggtc cgcacgcatc cgtggatcga gcgcgtgact
     1981 ctctcgaagc gtcttcccga cggtctctcg attcgcgtca cggagaagaa ggcggtcgcg
     2041 ctctaccgcg acgcgtcggg tctgtcgtgg ctctcttccg acgggcaccc gatcgcttcc
     2101 tacgatccgc gcgcggactc gaccgagtac gtgctcgtgt cgggagacct gcgcgctctt
     2161 cccgacgcgg tcgcgctgct cgacgacatt cgctctgcgc ccgcggaata tttctccgcg
     2221 ctctccgaaa tttccgcgct cccggacggt ggtttcggta tcatggatac gatcttccga
     2281 aagccggttc gtgttcttcg gcgggacgct cccgcgaaga ttcgcgcgct tctggaagcg
     2341 cgcagtttca tcgagtctcg tgggtgggag gcgcgggcaa tcgatctccg ttttgcggat
     2401 cgaattgtgt tggtcggagc ctacggggcg gggaactccc tatgagccgg aaatcggaaa
     2461 tcggtaatcg cgaatcgaag atcgcctgcc ctccgtccga ttgccgactt tcgactaccg
     2521 cttgccgggc gtcctgatgg ccaagcctga gaacttcctc gtctcctgcg acatcggcag
     2581 ctcgaagatc tgcgtgctga tcggcgagca gaacgcccat tccgcgctcg atgtgatcgg
     2641 aaaaggggcc gcccccaacc gcgggacgcg caagggcaac atcatcaacg tcgacgccac
     2701 gatcgaggcg atcaagcgcg ctgcggaaga agcggagat
//
LOCUS       DASWAV010000102         1827 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U2665
            k141_645353, whole genome shotgun sequence.
ACCESSION   DASWAV010000102 DASWAV010000000
VERSION     DASWAV010000102.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960316
            Sequence Read Archive: SRR10260007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 1827)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1827)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:47:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,929
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1827
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_645353"
                     /isolate="SMAG_U2665"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.36444 N 132.31889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            170..934
                     /gene="ricT"
                     /locus_tag="VF958_02180"
     CDS             170..934
                     /gene="ricT"
                     /locus_tag="VF958_02180"
                     /inference="COORDINATES: protein motif:HMM:NF041131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="regulatory iron-sulfur-containing complex
                     subunit RicT"
                     /protein_id="HEX9147948.1"
                     /translation="MSGCGGCGIGNRLNPGGLYVGIRINENEDGKAPFCDTQGITVRK
                     GERVVVEGEAGFEYAQVTNLKPMILKQCQLRDARRMVRIATDADNASYQAKLEREKRF
                     YFDTKRLALEKNLALKLIRTEETFDGRKVTVYYTSETRIDYRKLVQDLAAIHPTRIEM
                     RQVGPRDETRIRGGIGPCGLTLCCSTFLKSFHPVTIKMAKNQNLSLNPSKISGMCGRL
                     MCCLAYEEVGYRTAGPPAPPREAGVKAQNFQGDTPV"
     gene            980..1348
                     /locus_tag="VF958_02185"
     CDS             980..1348
                     /locus_tag="VF958_02185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020258909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9147949.1"
                     /translation="MATNRNTGAYRPDDDAPGNRKLIRPNLSEMADRYPTRSFQQRKQ
                     VPPEQTNAESYYYLKQMAAKTPMVVVLTDGQEVRGWIEWYDKACLKVNRDRGPNLLIP
                     KASIKYMYKQTEEEQRGGNR"
     gene            complement(1405..>1827)
                     /locus_tag="VF958_02190"
     CDS             complement(1405..>1827)
                     /locus_tag="VF958_02190"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012526858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006486 - protein glycosylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HEX9147950.1"
                     /translation="RTMEADAEGRLPEIAHLNEMSGPVFKAARDPRLTFVGRILRRLS
                     IDELPQLWNVVVGDMSLVGPRPPLPEEVARYEPWQRRRLSMAPGLTCLWQVSGRSEVD
                     FDRWMELDLKYIDTWSPILDLKILMKTVPAVLTGRGAR"
BASE COUNT          367 a          576 c          594 g          290 t
ORIGIN      
        1 gaccctcgag cgcgaagtcg tccgcaagga cgcgaagctc cagaaggagc gccggcaggg
       61 tggcccgccc tcccgatgac cccaacccgt gctaaggtaa gcctttagcg cgaagcttcc
      121 ggccggcggc tcgacgcttc cggctgatgg ctccccgggg gggattgaaa tgtcaggttg
      181 cggcggctgc gggatcggca atcggctgaa tcccggcggc ctttacgtcg ggatacgaat
      241 caacgagaac gaggacggga aagccccgtt ctgcgacacc cagggcatca ccgtccgcaa
      301 gggcgagcgc gtcgtcgtcg agggcgaggc ggggttcgag tacgcccagg tgacgaacct
      361 gaagcccatg atcctgaagc aatgccagtt gcgcgatgcc cggcggatgg tgcgcatcgc
      421 gacggacgcg gataacgcga gctaccaggc aaagctcgaa agggagaagc gcttctactt
      481 cgacaccaag cggctcgcgc tcgaaaagaa cctcgcgctg aagttgatcc ggaccgagga
      541 gacctttgac ggccggaagg tcaccgtcta ctacacctcc gagacccgga tcgactatcg
      601 caagctcgtg caggaccttg cggccatcca cccgacccgg atcgagatgc gccaggtggg
      661 cccgcgggac gagacccgca ttcgcggagg gatcggtcct tgcgggttga ctctctgttg
      721 ctcgaccttc ctgaaatctt ttcacccggt cacgatcaag atggccaaga accagaatct
      781 ctcgctaaat ccttcaaaaa tatcggggat gtgcggacgg ctgatgtgct gcctggcgta
      841 cgaggaggtg ggttaccgga cggccggacc ccccgcgcct cctcgtgaag ccggggttaa
      901 ggcacagaat tttcaagggg acactccggt ttaatggagc taagagtccc gccttttacg
      961 gggacgtgga gagacagccg tggcgacgaa ccgcaacacc ggggcctatc ggcctgacga
     1021 tgacgctccc ggcaaccgca agttgattcg accgaacctc tcggaaatgg ccgaccgcta
     1081 cccgacgcgc agcttccagc agcgaaagca ggtgccgccg gagcagacga acgccgagag
     1141 ctattactac ctgaagcaga tggctgcgaa gacgcccatg gtcgtcgtgt tgaccgacgg
     1201 tcaggaggtc cgcggctgga tcgagtggta cgacaaggcc tgcctgaagg tgaaccgcga
     1261 ccggggtccg aacctgctca tccccaaggc ttcgatcaag tacatgtaca agcagaccga
     1321 ggaagagcag cgggggggaa accggtaagt ccggttcgac gttcgagatt ttcgaggccc
     1381 gggaagatcc cgggcctttt ccattcagcg cgcgccgcga cccgtcagca ccgccggcac
     1441 ggtcttcatc aggatcttga ggtcgaggat cggcgaccag gtgtcgatgt atttcaggtc
     1501 gagctccatc caccggtcga agtcgacttc gctccgcccc gacacctgcc agaggcacgt
     1561 cagccccggc gccatcgaga gccggcggcg ctgccagggc tcgtagcgcg cgacctcctc
     1621 gggcagcggc ggacgcggtc cgacgaggct catgtctccc acgacgacgt tccagagctg
     1681 gggtaactcg tcgatcgaga gccggcggag gatgcggccg acgaacgtca ggcgcggatc
     1741 gcgcgccgcc ttgaagaccg ggccgctcat ctcgttcaag tgagcgatct cgggcagccg
     1801 cccctccgcg tccgcctcca tcgtgcg
//
LOCUS       DASWAV010000103         4706 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U2665
            k141_405354, whole genome shotgun sequence.
ACCESSION   DASWAV010000103 DASWAV010000000
VERSION     DASWAV010000103.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960316
            Sequence Read Archive: SRR10260007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 4706)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4706)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:47:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,929
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4706
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_405354"
                     /isolate="SMAG_U2665"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.36444 N 132.31889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1398
                     /locus_tag="VF958_02195"
     CDS             <1..1398
                     /locus_tag="VF958_02195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9147951.1"
                     /translation="SSITTDYSFDVVRWLAARFPRRVRLDWEDQEDTDRLRAFLPRFL
                     PLLEEEASEDANVPYLEWLCAASPDRRRDLAWLIDRIEALPLPHADRAERFEALRVPI
                     TWDLGDGPVTRTRMRWRAPEVFFHAGPLIARRDVSLEDMVAAPKLPIRKLSRAQGERV
                     VELTRRATALRYREYYGFTYADPASVRAARAGRGVEIFLMGLERDRRLPLRAGFAGFI
                     LKNGVPVGYIEGLAFFERIEIGFNIYYTFREGESAWIFGRVLRLLKQVYGVTSFSIDP
                     YQLGHENPEAIESGAFWFYRKLGFRPTSRAVENLLRAEERRISADPSYRSSPRALRRL
                     VTGNLLYEAQAGRAGDWDRFHIRNVGLAVQRRMRRRYGGDAGRARREAATRVARALGM
                     NVPRRPEERKAFTDLALALDLIPDIARWTREEKSALARITRAKAGREEVAYLRGLQRH
                     EKLRAALIRLGSVAH"
     gene            complement(1384..2388)
                     /locus_tag="VF958_02200"
     CDS             complement(1384..2388)
                     /locus_tag="VF958_02200"
                     /EC_number="1.2.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014099247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HEX9147952.1"
                     /translation="MEQPPGAAASRAETFYVDAIREALLEEMERDERVFLLGEDVGVY
                     GGAFKVTDGLIERFGEERVLDTPISETAIVGAAVGAAIAGLRPVAEMQFADFISCAFD
                     MITNFAAKSRYRTGCGVPMVIRAPAGGGVHGGPFHSQNPEAYFAHTPGLKIVQPSTAH
                     DAKGLLKAAIRDDDPVLYFEHKFLYRRAREVLPQEDYVVPIGKAAVRRAGRDLSILTY
                     GAMVWTALDAARTLEAEGIDAEVVDLRTLFPLDEETILESVARTSRAIVLHEATRTGG
                     IGAEVAAVLSERCFEALDAPVLRVTAPDTPVPFSPPMEAFFLPNAEKVCRAARALVGY
                     "
     gene            complement(2388..3395)
                     /locus_tag="VF958_02205"
     CDS             complement(2388..3395)
                     /locus_tag="VF958_02205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007222058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent dehydrogenase
                     E1 component subunit alpha"
                     /protein_id="HEX9147953.1"
                     /translation="MPDLTLPQLVDLYRLMRRNRSLEERLVHLYRQGKVVGGFYRSLG
                     QEATSVGSAYALETGDVLGPLIRNLGAVLVMGYTPLEVLTQHLGRASSPSGGKEGNLH
                     FGRPERGLVSSISMLGELVPVMAGVALAARLQRKRTVAMTYIGDGGTSTGAFHEGLNF
                     AAVQKLPLVVIVENNGWAYSTPFRKQTAAKSLADRAPAYGIPGTSVDGNDVVAVYEAS
                     REAVRRARSGGGPTLVEAMTYRMKGHAEHDAQAYVPREELDDWARRDPIARHAERLLA
                     SGDVTAADLEAIDRSIAEELDRDVAEAERCPYPAADHALADVYAGHAPENEPSFLRRL
                     L"
     gene            3437..4408
                     /locus_tag="VF958_02210"
     CDS             3437..4408
                     /locus_tag="VF958_02210"
                     /inference="COORDINATES: protein motif:HMM:NF025017.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEX9147954.1"
                     /translation="MPPIVSVVIASDRVEGSLAECLRSLERQGPSTSFEVLVASASPW
                     RSGVSTPFPFEWVPAESRNPAFRRNVAARRSRGAVLAFLDDDAEAEAGWLEAGARAVE
                     SADIAGGPDLGPAGAPYLERISDLLLATPIIGSGIPAHERRARGGPVGSPHDVALCNL
                     FVRREAFDALGGFDESLGYVGEDTEFVRRALDGGFRVLFDPSVRVRHRRRPFPAGYVA
                     QRWRYRVKTGRLLVERPGLHARGRIAAFLSAGFAATAGIALFGLPFLLPAALAYAAAT
                     CALSFPIWKDDPSLLPAVPFAFAVHHATYFAGLIAGIARGLFTVAVR"
     gene            4405..>4706
                     /locus_tag="VF958_02215"
     CDS             4405..>4706
                     /locus_tag="VF958_02215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HEX9147955.1"
                     /translation="MNELGLAGQWLRGRRTLPRYLILGMTNLCNSKCVTCFYWDHLNV
                     NRHLEMSLDDYSRALADLPSLYSVVLTGGEPTLNRDLPEVTKLLYRRHGAANVTMPT"
BASE COUNT          694 a         1638 c         1598 g          776 t
ORIGIN      
        1 agctcgatca cgacggacta ctccttcgac gtcgtgcggt ggctcgcggc gcgcttcccg
       61 cggcgcgttc gcctcgactg ggaggaccag gaggacacgg accggctgcg ggcattcctg
      121 ccccgcttcc ttccgctcct cgaggaggag gcgagcgagg acgcgaacgt gccttacctc
      181 gagtggctct gcgcggcgtc gccggaccga cggcgcgacc tcgcgtggct gatcgaccgg
      241 atcgaggcgc tcccgcttcc gcacgcggac cgcgccgagc ggttcgaggc gctgcgggtg
      301 ccgatcacgt gggatctcgg cgacgggccg gtcacgcgga cgcgaatgcg gtggcgcgcg
      361 cccgaagtct tctttcacgc gggtcctctg atcgcccgcc gcgacgtctc gctcgaggac
      421 atggtcgccg cgccgaagct cccgatccgc aagctctcgc gcgcacaggg cgagcgcgtc
      481 gtcgagctta cccgcagggc gaccgcgctc cggtatcgcg agtactacgg attcacgtat
      541 gccgatccgg cgtcggttcg ggccgctcgc gccggccggg gggtcgagat cttcctgatg
      601 ggattggaac gagatcgccg attgccgctg cgcgcgggat tcgccggctt catcctgaag
      661 aacggcgtgc cggtcggcta catcgaaggt ctcgccttct tcgagaggat tgagatcggt
      721 ttcaacatct actacacgtt ccgcgagggg gagtccgcct ggatcttcgg ccgcgtcctg
      781 cgtctattga aacaggtgta cggcgtgacc tcgttctcga tcgatcctta ccagctcggg
      841 cacgagaacc cggaggcgat cgaatcgggc gccttctggt tctaccgaaa gctcggcttc
      901 cggccgacgt cccgggcggt cgagaacctc ctccgcgccg aggagaggcg gatttcggct
      961 gacccttcgt accgttcctc gccgcgcgct ctccggcgac tcgtcacggg caacctgctc
     1021 tacgaggcgc aagcgggtcg cgccggtgac tgggaccgct ttcacatccg caacgtcggt
     1081 ctcgccgtcc agcgccggat gcgccggcga tatgggggcg acgccggccg cgcccggcgg
     1141 gaggcggcga ctcgagtcgc gcgcgccctc ggaatgaacg tgcctcggcg tcccgaggaa
     1201 cgaaaagcat tcaccgatct ggcactggcg ctggacctga ttccggacat cgctcgatgg
     1261 acacgcgagg aaaagagcgc gctcgcccgg atcacgcggg ccaaagcggg tcgcgaggaa
     1321 gtcgcctacc tgcggggcct ccagcggcac gagaagctcc gggcggcgct cattcgactg
     1381 gggtcagtag cccactagcg cgcgcgcggc ccggcagacc ttttccgcgt tgggcaggaa
     1441 gaacgcctcc atcggagggg agaaggggac cggggtgtcg ggcgcggtca cccgcaaaac
     1501 cggcgcgtcc agcgcttcga agcatcgctc ggagagaacc gccgcgacct ccgcgccgat
     1561 ccctccggta cgggtcgcct cgtgcagcac gatcgcgcgg ctcgtccgcg caacggattc
     1621 gaggatggtc tcctcgtcca gtggaaagag cgttcgcagg tcgacgacct ccgcgtcgat
     1681 cccctcggct tccagggtgc gcgccgcgtc gagcgccgtc cagaccatgg cgccgtaggt
     1741 caggatcgag agatcgcggc cggcgcggcg taccgcggcc ttgccgatcg gcacgacgta
     1801 gtcctcctgc ggaaggacct cccgggcgcg gcggtagagg aacttgtgct cgaagtacag
     1861 gaccggatcg tcgtcccgga tcgctgcttt cagcaggccc ttggcgtcgt gggcggtcga
     1921 tggctgcacg atcttcaagc ccggcgtgtg cgcgaaatac gcctccggat tctgcgaatg
     1981 gaaggggcct ccgtgaaccc cgccgccggc cggcgcgcgg atcaccatcg ggacgccaca
     2041 gccggtgcgg tagcgcgact tcgccgcgaa gttcgtgatc atgtcgaatg cgcaggagat
     2101 gaaatccgcg aactgcatct ccgccaccgg ccggagaccg gcgatcgccg cacccacggc
     2161 ggcgcccacg atcgcggtct cggagatcgg cgtgtcgagc acccgctcct cgccgaatcg
     2221 ctcgatcagg ccgtcggtca ccttgaaagc gccgccgtaa acgccgacgt cttccccgag
     2281 caggaagact cgctcgtcgc gctccatctc ctcgaggagc gcttcacgga tcgcgtcgac
     2341 gtagaacgtc tccgcgcgcg acgcggcagc gccgggcggc tgttccatca gaggagtctc
     2401 cgcagaaaag agggctcgtt ctccggggcg tggccggcgt agacgtcggc cagtgcgtgg
     2461 tcggccgcgg gatacggaca cctctccgct tccgcgacgt cgcgatcgag ctcttcggcg
     2521 atcgagcggt cgatggcctc gaggtccgcc gcggtgacgt cgcccgacgc gagaagcctc
     2581 tcggcatgcc gcgcgatcgg gtcccgccgg gcccagtcgt cgagttcctc tcgcggcacg
     2641 tacgcctgcg cgtcgtgctc cgcgtgtcct ttcatccgat acgtcatcgc ctccaccagg
     2701 gtcggacctc cgccggaccg cgcgcgccgg acggcctccc gcgaggcttc gtacacagcg
     2761 acgacgtcgt tgccatccac gctcgtccct ggtatcccat aggccggcgc ccggtcggcg
     2821 aggctcttcg ccgcggtctg cttgcggaac ggcgtcgagt atgcccagcc gttgttttcg
     2881 acgatgacga cgagcggcag cttctggacc gccgcgaagt tgaggccctc gtggaacgcg
     2941 cccgtcgacg ttcctccgtc gccgatgtac gtcatcgcga cggtgcgctt gcgctggagg
     3001 cgcgccgcga gagcgacccc cgccatgacg ggaacgagct ccccgagcat cgagatcgac
     3061 gaaacgaggc cgcgctccgg gcgcccgaaa tgcaggtttc cctccttgcc tccggacggc
     3121 gatgacgcgc ggccgaggtg ctgcgtgagc acctccaggg gcgtgtaccc catgacgaga
     3181 acggcgccga ggttgcggat gagcggcccg agcacgtctc ccgtctcgag ggcgtacgcg
     3241 ctgcccacgg acgtcgcctc ctgcccgagt gatcggtaga acccgcccac gaccttcccc
     3301 tgccgataga ggtggacgag cctctcctcc agggagcggt tgcgccgcat gagccggtag
     3361 aggtcgacga gttgggggag agtcaggtcc ggcattggga acggtcgcga ttatacgggc
     3421 gggctaagat gacccggtgc ctccgatcgt ctccgtcgtg atcgcgtccg atcgcgtgga
     3481 gggctctctg gcggagtgcc tccgctcgct cgagcggcag gggccgtcga cgtcgttcga
     3541 agtcctcgtc gcttccgcgt cgccgtggcg ctccggggta tcaactccat ttccgttcga
     3601 atgggtgccc gccgaatcac gcaaccccgc ctttcgccgg aacgtcgccg cacgccgttc
     3661 gcgcggagcg gtgctcgcgt ttctcgacga cgacgcggaa gcggaagcgg ggtggctcga
     3721 ggccggcgca cgggcagtcg agagcgcgga catcgccggc ggcccggacc tcggacccgc
     3781 gggggcgcca tatctcgaaa ggatctccga cctcctgctc gcgaccccga tcatcgggag
     3841 cggcataccg gcgcacgaac gccgggctcg cggcggtccg gtcggatcgc ctcacgatgt
     3901 cgcgctgtgc aatctcttcg tgcggcgaga ggccttcgac gctctcggcg gcttcgacga
     3961 atcgctcggg tacgtggggg aggacacgga gttcgtgaga cgcgcgctcg atggagggtt
     4021 ccgcgtcctc ttcgacccga gcgttcgggt gcgacaccgc cgacgtccgt ttcccgccgg
     4081 atacgtcgcg cagcggtggc gctaccgcgt caagacggga aggctcctcg tcgaaaggcc
     4141 cgggctccat gcgcgggggc ggatcgccgc cttcctctcc gcgggattcg ccgccaccgc
     4201 cggaatcgcc cttttcggac ttccgtttct cctgcccgcg gcgctcgcgt acgccgccgc
     4261 gacgtgcgcc ctctcgtttc cgatctggaa agacgatccg tccctcctgc ccgcggtccc
     4321 gttcgcgttc gccgtccacc acgcgacgta tttcgccggg ctgatcgccg gaatcgcgag
     4381 gggcctcttc acggtcgccg tacggtgaac gaactcggtc tcgccgggca gtggctccgg
     4441 ggacgacgca cgctgccgcg gtacctcatt ctcggaatga cgaacctgtg caacagcaag
     4501 tgcgtgacct gcttctactg ggaccacttg aacgtgaacc ggcacctcga gatgtccctc
     4561 gacgactatt cgcgggctct cgccgacctg ccctcgcttt acagcgtcgt cctgacgggc
     4621 ggcgagccta cgttgaaccg cgacctgccg gaggtgacga agctcctcta ccggcgccac
     4681 ggcgcggcga acgtgaccat gccgac
//
LOCUS       DASWAV010000104         2094 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U2665
            k141_365871, whole genome shotgun sequence.
ACCESSION   DASWAV010000104 DASWAV010000000
VERSION     DASWAV010000104.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960316
            Sequence Read Archive: SRR10260007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2094)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2094)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:47:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,929
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2094
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_365871"
                     /isolate="SMAG_U2665"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.36444 N 132.31889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(113..1738)
                     /locus_tag="VF958_02220"
     CDS             complement(113..1738)
                     /locus_tag="VF958_02220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9147956.1"
                     /translation="MRFARGGMLVACLVLSQGVVFAQYEVGIPVPNWTVPPFHGAGAE
                     RGLSPMSDISLGVAFVAMLPCRVFDTRGPNGPYGGPRLLANTTRNFDIDSGPCTGIPT
                     GVDAYSMNFGAILPDGANSFITIWPTGFGMPTVSSINPIQGAVVANAAIVPAGDAGSI
                     SVFPNTGVHLYGDINGYFTDQFNSGIFLHAVSSNVAPAIIGDNNSMLGSAFGVLGHIN
                     TTTTGSASAGVRGMNDGLGLAGVGVWGSHAAGGWGTFGEAVSGVGVYGKATATTGANY
                     GMRAETASTFQNSAGIRASNGPAPPSWTILSAGVRGEAPSLGFGVVGMSRGWAGVSGY
                     FLDTAGNISAGADLGYTPTVGLNVTGSTQATGVKNFIEPHPTDPSKVIRYISLEGNEA
                     GTYFRGRGKFQNGIAMIDVPEDFRIVTDSEGLGIQVTPIGQMATVAVEAIGLDRIVVR
                     GSRDVEFFYTVNGIRSAYKDAGPIVENDKAFVPRSPDATMPEAYPAGIRQRLISNGIY
                     RPDGTVNMETARRLGWDKEWERRERPAPQPASD"
     gene            complement(1864..>2094)
                     /locus_tag="VF958_02225"
     CDS             complement(1864..>2094)
                     /locus_tag="VF958_02225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9147957.1"
                     /translation="EKVFVPRSADDRMPDSYPAVVRERLIANGTYNSDGTVNMETARR
                     LGWDKEWERRERPAPQPASRSGLRRGSRNRPG"
BASE COUNT          380 a          698 c          648 g          368 t
ORIGIN      
        1 ggccgaaact aggctagcga ggaaaaaagt tcaccgacgc gagaagcagg agaaatcgta
       61 tccaacaaaa aggccgcggg atcgccgcgg cctcgattcg gaagataaga cttcagtcgg
      121 acgcgggctg cggggccgga cgttcgcgcc tctcccactc cttgtcccag ccgagccgtc
      181 gtgccgtctc catgttgacg gtaccgtcgg gtcggtagat tccgttcgaa atcagacgct
      241 ggcggatccc agcgggataa gcctcgggca tcgtggcgtc gggcgagcgc ggcacgaacg
      301 ccttgtcgtt ctcgacgatc ggtccggcgt ctttgtacgc gcttcggatt ccgttgactg
      361 tgtagaagaa ctcgacgtcc cgcgagccgc ggacgacgat gcgatcgagc ccgatcgcct
      421 cgactgccac ggtcgccatc tgcccgatcg gagtgacctg gataccgagc ccttcggaat
      481 ccgtcacgat tcgaaaatcc tctggaacgt cgatcatcgc gatgccgttc tgaaattttc
      541 cgcgaccgcg gaagtacgtc cccgcttcat tcccctcgag agagatgtac cgaatcacct
      601 tcgaagggtc cgtcggatgc ggctcgatga agtttttcac gcctgtcgcc tgcgtggagc
      661 cggtcacgtt caatcccacc gtcggggtgt agccgaggtc cgcgcccgcc gagatatttc
      721 cggcggtgtc caggaaatac ccgctcacgc ccgcccaacc tctcgacata ccgacgaccc
      781 cgaaacccag gctcggggct tctccccgga ctccggcact gagaatggtc cagctcggcg
      841 gagcgggacc attgctggcg cggatgccgg ccgagttttg aaaggtgctc gccgtctccg
      901 cccgcattcc gtaattcgcc cccgtcgtcg cggtggcctt gccgtagacg ccgacgccgc
      961 tgacggcctc accgaacgtg ccccagccgc ccgcggcgtg ggagccccag acaccgaccc
     1021 ccgcgagtcc gagtccgtcg ttcatgccgc ggacgcccgc ggaggcggat cccgtggtcg
     1081 tcgtgttgat atggcccagt acgccgaagg cggagccgag catcgagttg ttgtcaccga
     1141 tgatcgccgg ggccacgttg cttgagacgg cgtggaggaa gatgcccgag ttgaactggt
     1201 cggtgaaata gccgttgatg tctccgtaga ggtggactcc cgtgttcgga aaaacgctga
     1261 tcgagcccgc gtctcccgcc ggcacgatcg ccgcgttggc cacgacggct ccctggatcg
     1321 ggttgatcga agagaccgtc ggcatcccga agcccgtcgg ccagatcgtg atgaacgagt
     1381 tcgctccgtc cggcaggatc gccccgaagt tcatcgagta ggcgtcgacg ccggtcggga
     1441 ttcccgtgca cggcccgctg tcgatgtcga aattgcgcgt cgtgttggcc agaagcctcg
     1501 gacccccgta cggcccgttg ggcccccgcg tgtcaaatac ccggcacggc agcatcgcca
     1561 cgaacgccac gccgagagaa atgtccgaca tcggcgagag cccgcgttca gcgccggcgc
     1621 catgaaacgg cggtaccgtc cagttcggca ccgggattcc gacctcgtac tgagcgaaga
     1681 cgactccctg cgaaaggacc agacaagcca ccagcattcc cccccgagcg aatcgcatcg
     1741 gcgacctcct tcctgaatcc ggcgtttcct cactctaccg gccatgagaa gaatcgtatc
     1801 gtctaagaag acggccgcgg aatctcagcg accttccgca agccgggccg acttcgacgg
     1861 tgtctatccg ggacgattcc gagaacctcg acgaaggccg gatcgggaag cgggctgcgg
     1921 cgccggtcgc tcgcgcctct cccactcctt gtcccagccg agacgacgcg cggtctccat
     1981 gttcaccgta ccgtcggagt tgtaagtccc gttcgcgatc aggcgctccc ggacgaccgc
     2041 ggggtacgaa tcgggcatcc ggtcgtcggc agatcgggga acgaagactt tctc
//
LOCUS       DASWAV010000105         3096 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U2665
            k141_126118, whole genome shotgun sequence.
ACCESSION   DASWAV010000105 DASWAV010000000
VERSION     DASWAV010000105.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960316
            Sequence Read Archive: SRR10260007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 3096)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3096)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:47:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,929
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3096
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_126118"
                     /isolate="SMAG_U2665"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.36444 N 132.31889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..194)
                     /locus_tag="VF958_02230"
     CDS             complement(<1..194)
                     /locus_tag="VF958_02230"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="catalyzes the formation of L-threonine from
                     O-phospho-L-homoserine; Derived by automated computational
                     analysis using gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HEX9147958.1"
                     /translation="MFSFFAHLVCTKCGRVHERDSRFGVCPAPECGGPLFARYHDRPL
                     DRDAFEDRPRTMWRWREMMP"
     gene            352..729
                     /locus_tag="VF958_02235"
     CDS             352..729
                     /locus_tag="VF958_02235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9147959.1"
                     /translation="MELNDHLRDMLKELGQAINESISGSGRVHDSIQRIRDEGYNLYM
                     VLDAKVGVNRREGRGGPRSRSGAARHETAGEERAAFRINVKDLRFLKSVGIDPTRKVR
                     NRRGTLRTRLTRPVRRVKSLKSD"
     gene            753..1958
                     /gene="rlmN"
                     /locus_tag="VF958_02240"
     CDS             753..1958
                     /gene="rlmN"
                     /locus_tag="VF958_02240"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: protein motif:HMM:TIGR00048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016433 - rRNA (adenine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HEX9147960.1"
                     /translation="MTAPVPSPHALGLSRSQWEALLESWGAPAYRGRQVFDAIHRRGA
                     RSAAAIHELPRELRERVEREMPIGLPEIVRREVSRDGSVKYGLRLGDGALVEAVYMPG
                     DRGNADVNEFEDARAVGPGSVPSPRPSSGGRGSPGGRFTVCLSSQTGCAVDCAFCVTG
                     RLGGGRNLTAGEIVGELCAVVDDAGRSREGLRVVFMGMGEPFLNPEGVLGALDVLFEI
                     LPVRRVTVSTSGITPAFETFAARPMRPNLAVSLNAADSETRAALMPITRVHPLDGLLR
                     AMRAWPLESRRRITVEYVLIAGRNDSPEEAQRLARLLRGIRAKVNVIPLNEDPAYLPG
                     WKRPDEGAIDRFAAALKAAGVPVTVRRSRGPDASAACGQLKGRTEDPRKRISGSGPKV
                     LPEKRGPSA"
     gene            complement(1908..2927)
                     /locus_tag="VF958_02245"
     CDS             complement(1908..2927)
                     /locus_tag="VF958_02245"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEX9147961.1"
                     /translation="MRGRRSSLRPGRVARSVLRAAAFASAAGAFFLAASWLAARRLAD
                     RLVSASGLGPTESTREELLAALALAAPVVEEFRHKGSARDPVELAATFASPGDPSSRS
                     TIVFLHGKGGHSSEWRPDALRALALGYNVLVPDLRGHGASGGRYMTFGFLEKDDLGNA
                     IDAACGLWNIDPARIGLHSCSAGSAVALELATHRPVRAIWLESPFANPREMARHYLAA
                     ASGIPAPFLSLTTRWAVARTLARVKRDLGLEGASGGLESADPLAAMARLRCPVSLVYG
                     GEDRLIPPRFVERLIDALPPGSEVFHAEGAGHCHHANEAAAVAREEYLRRWTAFFGQH
                     LRARA"
BASE COUNT          520 a         1048 c         1093 g          435 t
ORIGIN      
        1 aacggcatca tctcgcgcca tcgccacatc gtgcgcggcc ggtcctcaaa cgcatcgcga
       61 tcgagcggac gatcgtgata ccgcgcgaag agcggtccgc cgcactccgg cgcgggacac
      121 actccgaagc ggctgtcgcg ctcgtggacg cggccgcact tcgtgcagac gagatgcgcg
      181 aagaaggaga acatcgcagg aaagctatca gcgatcagcg cgaatcgaga tcggaattgc
      241 ggatcgcatg cgcaccgata gcgcgagagg aggcaaaaca gaatagcacc aatccgagga
      301 tcggaccatg cttgacgctc ccgcgaagcg gaagggataa tcgccgcggt catggaattg
      361 aacgatcacc tgcgcgacat gctgaaggag ctcggtcagg cgatcaacga gtcgatctcc
      421 ggctcgggtc gcgtgcacga ctccatccag cggattcgcg acgagggtta caacctctac
      481 atggttctcg atgcgaaggt cggcgtgaac cggcgcgagg ggcgcggagg cccgcgctcg
      541 cggagcggcg ccgcgcggca cgagacggcg ggagaggagc gcgccgcgtt ccggatcaac
      601 gtgaaggacc tgcggttcct gaagtccgtc ggcatcgatc ccacccggaa ggtccgaaac
      661 cgccgaggca ccctgcggac gcggctcacg cgcccggtgc gccgcgtcaa gtcgctcaag
      721 agcgactaac attccagcgg ccacgcgccg ccatgaccgc tcccgtcccc tctccgcacg
      781 ctttgggact ctcgcgctcg cagtgggagg cgcttctcga gagctgggga gcgccggcgt
      841 atcgcggccg acaggtcttc gacgcgatcc accgccgcgg ggcgcggtcc gccgccgcga
      901 ttcacgagtt gccgcgcgag ctccgggagc gtgtcgagcg cgagatgccg atcgggctgc
      961 ccgagatcgt gcggcgagag gtctcccgcg acggcagcgt gaagtacggc cttcgactcg
     1021 gcgacggcgc gctcgtcgag gcggtctaca tgcccggaga ccgcgggaac gccgacgtca
     1081 acgaattcga agatgcgcgg gcggtgggtc ccggttcagt cccctcaccc cgaccctctt
     1141 ccggagggag agggagtccg gggggcaggt tcaccgtttg cctttcgtcg cagaccgggt
     1201 gcgccgtcga ctgcgctttc tgcgtcacgg ggcgcctggg cggcggacgc aacctgaccg
     1261 ccggtgagat cgtcggcgag ctctgtgccg tcgtggacga cgccggccgc tcccgcgagg
     1321 gccttcgcgt cgtgttcatg gggatgggcg agcccttctt gaatcccgag ggggtgctcg
     1381 gcgctctcga cgttctcttc gagatcctgc cggtgcgaag ggtgacggtc tcgacgtcgg
     1441 gcatcacgcc ggccttcgaa accttcgcgg cacggccgat gcgcccgaac ctcgccgttt
     1501 cgctgaacgc cgccgattcg gagacgcgcg ccgcgctgat gccgatcacc cgcgtgcatc
     1561 ccctcgacgg tcttctccgc gcgatgcgcg cgtggccgct cgagtcacgg cgccggatca
     1621 ccgtcgagta cgtcttgatc gcggggagaa acgattcgcc cgaggaagct cagcggctcg
     1681 cacgtctcct ccgcgggatc cgggccaagg tcaacgtcat tcccctgaac gaggatcccg
     1741 cgtatctgcc cggctggaag agaccggacg aaggggcaat cgaccgattc gcggcggcgc
     1801 tcaaggccgc gggcgttccc gtcaccgtgc gacggagtcg aggccccgac gcctccgccg
     1861 cgtgcgggca gttgaaaggg cggacggagg acccgcgaaa gaggatttca ggctcgggcc
     1921 cgaaggtgct gcccgaaaaa cgcggtccat cggcgtaggt actcctcccg cgcgacggcg
     1981 gcggcctcgt tcgcgtggtg gcagtgcccg gcgccttcgg cgtggaagac ctcgcttccg
     2041 gggggcagtg cgtcgatcag gcgttcgacg aagcgcggcg gaatgagacg atcctccccg
     2101 ccgtagacga gcgagacggg gcagcgaagg cgggccatcg cggcgagcgg atccgcgctc
     2161 tcgagtcccc ccgaggcgcc ttcgagaccc aggtcgcgct tcacccgggc gagcgtgcgg
     2221 gcgacggccc atcgggtcgt gagcgagaga aatggcgcag ggatcccgct cgccgcggcc
     2281 aggtagtgac gcgccatctc gcgagggttc gcgaacggcg actcgagcca gatcgcccgg
     2341 acggggcgat gcgttgcgag ttcgagagcg acggcggagc ctgccgaaca tgagtgcagc
     2401 ccgattcgcg ccggatcgat gttccacagc ccgcacgcgg cgtcgatcgc attgccgaga
     2461 tcgtccttct cgagaaaacc gaacgtcatg tagcggccgc ccgacgcgcc gtggccccgc
     2521 aggtcgggaa cgagaacgtt gtagccgagc gcaagcgcgc gcagagcgtc cggccgccac
     2581 tccgaggagt ggcccccctt gccgtgaagg aagacgatcg tcgaccgcga ggagggatcg
     2641 ccgggagacg cgaacgtcgc cgcgagctcg acggggtcgc gtgcactgcc cttgtgtcgg
     2701 aactcctcga cgaccggcgc agcaagcgcg agggccgcga ggagctcctc ccgcgtcgac
     2761 tcggtcgggc cgagtccgct cgccgagacg agccggtccg cgagccggcg cgcggcgagc
     2821 caggaggcgg cgagaaagaa cgcgccggct gccgacgcga aggcggcggc tctcaggacg
     2881 ctccgagcga cgcgtcccgg gcggagcgaa gagcgtcttc ctctcaaccc gacgctacgg
     2941 caatccgcgt gcggcccgaa ccttcccggc ctttttcccc aggagaccga cgacctcgac
     3001 cggcccgtgc acgtaggtct ggcaggcgag ccggaccgtt cgacccttcc accgcttccg
     3061 gatgaacgcg agcggtttac cgagccacca catcgc
//
LOCUS       DASWAV010000106         3414 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U2665
            k141_165865, whole genome shotgun sequence.
ACCESSION   DASWAV010000106 DASWAV010000000
VERSION     DASWAV010000106.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960316
            Sequence Read Archive: SRR10260007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 3414)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3414)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:47:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,929
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3414
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_165865"
                     /isolate="SMAG_U2665"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.36444 N 132.31889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..594)
                     /gene="lipB"
                     /locus_tag="VF958_02250"
     CDS             complement(<1..594)
                     /gene="lipB"
                     /locus_tag="VF958_02250"
                     /EC_number="2.3.1.181"
                     /inference="COORDINATES: protein motif:HMM:TIGR00214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033819 - lipoyl(octanoyl) transferase
                     activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl(octanoyl) transferase LipB"
                     /protein_id="HEX9147962.1"
                     /translation="MEALDSTGAVGGPVPTRRIEALWLGRIGYGRALVLQREAAARVA
                     RGGPETLLLLEHDPVFTLGRNASAADVLFTPERRRELGIELFEADRGGKVTFHGPGQL
                     VGYPILNLHPDRRDVRRYVRDLEEVLLRTLGDFGVSAERSPLPERLSSVWIGNEKIAA
                     IGVHLSRWITTHGFALNVTEEPLFYFRGIVPCGITDGG"
     gene            complement(564..1808)
                     /locus_tag="VF958_02255"
     CDS             complement(564..1808)
                     /locus_tag="VF958_02255"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HEX9147963.1"
                     /translation="MGAKALLVVLLLALVLRLVSAGIVGFSTVRFGDARAYLSAAETI
                     VREGRYPIRTDVFFFRPPGYPAFLAAATLGHPDKIALSKIATASVGALAAPLLALLSA
                     RLFRRRGVAIATGLFAAVHPAFLLTSSDVQSEPLFLALLLGAGYLLLAAVDRPSTTLS
                     LAAGGIAAMAALTRSSALALVPFLLAPLLDRRYPRRARAHLAGAAVVGFLFVLAPWTI
                     RNALVFGEFIPVNDAAGNAFYQGNSDWTLRFYDLRTREEYDRWVKAFNADMRRRSEEM
                     RRAGQESPTARSRAFAAQALAERRRDHAEWAKLLLLKGWDWLRPYPHPIFWPRGIVLG
                     VAGAYVLLFSSAAIGLARAPRPGVRLFVLAFLAVTMIAHVLIIVVWRYRVPYWDPVLL
                     LYGTFGAGTLLTGWKLSTRRAQ"
     gene            complement(1873..3357)
                     /gene="sucB"
                     /locus_tag="VF958_02260"
                     /pseudo
     CDS             complement(1873..3357)
                     /gene="sucB"
                     /locus_tag="VF958_02260"
                     /inference="COORDINATES: protein motif:HMM:TIGR02927.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase, E2 component,
                     dihydrolipoamide succinyltransferase"
BASE COUNT          590 a         1105 c         1191 g          528 t
ORIGIN      
        1 tccgccgtcg gtaattccgc acgggacgat cccgcggaag tagaagagcg gttcctccgt
       61 gacgttcagg gcgaacccgt gggtggtgat ccatcgggag aggtgaacgc cgatggctgc
      121 gattttttcg ttgccgatcc acacggaaga aaggcgctcc ggcagcggag accgctccgc
      181 ggagaccccg aaatctccca gggtccgcag gagcacctct tcgaggtcgc gcacgtaccg
      241 ccggacgtcg cgtcggtccg ggtggaggtt cagaatggga tagccgacca gctgtcccgg
      301 accgtgaaac gtcaccttcc cgccccggtc ggcttcgaag agctcgatgc cgagctcgcg
      361 ccgtctctcg ggagtgaaca gaacgtccgc tgccgacgcg ttccgaccga gggtgaagac
      421 cggatcgtgc tcgaggagga gaagcgtttc cggaccgccg cgggcgaccc gcgcggcggc
      481 ctcgcgctgg aggacgagcg cgcgtccgta gccgatgcgc ccgagccaca gcgcttcgat
      541 gcggcgggtc ggaaccggtc cgcctactgc gcccgtcgag tcgagagctt ccatcccgtc
      601 agaagtgtac ccgctccgaa cgtcccgtag agaaggagca cggggtccca gtagggcacg
      661 cgatatcgcc acacgacgat gatcagcacg tgcgcgatca tcgtgaccgc gaggaacgcc
      721 agaacgaaga ggcggacacc cggtcggggc gcccgcgcga gaccgatcgc agcggacgag
      781 aagagaagga cgtaggcgcc ggccacgcct aagacgattc cgcgaggcca gaagatcgga
      841 tgcggatagg gacggagcca atcccacccc ttgagtaaga ggagcttcgc ccattcggcg
      901 tgatcgcgcc gccgctcggc aagcgcctgt gctgcgaatg cgcgggaacg cgcggtcggt
      961 gattcttgcc cggcccgtct catctcctcg cttcgccggc gcatgtcggc gttgaaggcc
     1021 ttgacccacc ggtcgtattc ctcgcgggtg cgcaggtcgt agaaacggag ggtccaatcg
     1081 ctgttgccct ggtagaacgc gtttcccgcc gcgtcgttga ccggaatgaa ttcgccgaag
     1141 acgagcgcat tgcggatcgt ccagggcgcc aggacgaaca agaacccgac gaccgctgct
     1201 ccggccaagt gggcgcgcgc gcgccgtgga tagcggcgat cgaggagcgg cgcgaggaga
     1261 aacggcacga gggcgagcgc agaggagcgc gtgagagccg ccatcgccgc gatcccgccg
     1321 gccgcgagcg acaaagtcgt cgacgggcgg tcgaccgcgg cgagcaggag gtagcctgcg
     1381 ccgagaagca gcgcgagaaa gagcggctcg ctctggacgt cgctcgacgt cagcaggaaa
     1441 gccggatgga cggccgcaaa gagacccgtg gcgatggcga cgccgcggcg gcggaagagc
     1501 cgggccgaga gcagcgcgag aagcggggcc gccagagccc cgaccgaggc ggtcgcgatc
     1561 ttggagaggg cgatcttgtc ggggtgtccg agcgtcgccg ccgccaggaa cgccggatac
     1621 cctgggggcc gaaaaaaaaa gacgtcggtt cggatcggat atcgaccctc ccggacgatc
     1681 gtttcggcgg cggagagata ggcccgcgcg tctccgaagc ggaccgtcga gaatccgacg
     1741 atgccggcgc tcacgagccg gagcacgagg gcgagcagca atacgaccag gagcgccttc
     1801 gctcccatcc tggggacggt tccttgtccg gccgctatca gtgaaccgcg gatcaccgag
     1861 agctgaaagc tctcatccca gatcgaactc gcccttctcg agcgtcctct tgacgagagc
     1921 catgaaccga tcggcgtcgg aaccgtcgat caaacggtga tcgaaggaga gcgccaggta
     1981 acccaccgtt cggatcccga gcgcgtcgtt gccgttctcg tcggtgacga cgacgggccg
     2041 cttctcgatg gtcccgaccc ccaggatcgc gacctgcggc tgcggaatga tgggtgtgcc
     2101 gaagagcgac ccgaagaccc cgggattggt gaccgagaac gtgccgccct ggatctcttc
     2161 gggcctcaga cgcttcgtcc gcgcccgctc ggcgagatcc tgcgaagcgc gcgcgagccc
     2221 gaggatcgac ttttcgtcgg cgttccggat cacgggtacg atcaaccccc agtcgagcgc
     2281 gaccgccatc ccgaggtgaa tgtccttgtg gtagacgatg ttgtcgccgt cgatcgaagc
     2341 gttcacctgc gggaactcct tcagcgcctg gatcaccgca cggaagatga acggcaggta
     2401 cgtgagcttc acgccattct tctggaagaa agcatccttg ttctgttctc ggaggcgggc
     2461 gacgctggtg tagtcgacgt ggaagaccgt cgtgacgtgt gccgaagtcc tcttcgacag
     2521 gatcatgttc tccgcggtga tcctgcggat cttcgtcatc gcgacgactt cgtcccgatc
     2581 cgtgaccgga gtcgacggtc gcgccggggc agccgcgggc gccgcagcct caaccggccg
     2641 cgcagcctcg cgcaaaccgg cggacaccgt catgccggga gcgccggcga ccttgcggtt
     2701 ctcgatgtag gagagaatgt cgttcttcgt gacgcggctg taaataccgg tcccctcgat
     2761 ctcctggatg tcgactccgt gctccgccgc gatcttgcgg acgagcgggg aggaccgcag
     2821 gcgaattcgc tcctcgatcg attccacgct tccgccttcg ggctctcgaa cgggaacggc
     2881 gaccggggcg gccgtccgcg cgggtgccgg agccggagcc ggagcgggtg ctggaggcgg
     2941 cgcgggcctt tcgggcggcg gttccgtcgc tcgggcgggg gacgctgccg cagccggccg
     3001 ggcttcgggc gcggggggag cggcggctgt gggcgccggg ggagccgcgg ctgggggcgc
     3061 cggggagggc tcggacgctc ctgccgtcgc ggtcggggcg gagacggcgg cttcggacgc
     3121 ctcgccgatc atgccgacga cagtgttgat gggaaccgtc tgcccctcct tgacgcggat
     3181 ctcgacgagc gtgccggcga ccggcgaggg aatctccgcg tccaccttgt ccgtcgaaat
     3241 ctcgaagatc ggttcgtccc gcttgaccga ctcgccgacc ttcttcatcc acctcgtgat
     3301 cgttccctcg gcgatcgact cgcccatctg cggcatgatg atttcggtcg ccatcgcacc
     3361 tcctccggat ccaaatcaga atagcaccgt gcccgccgcc gttcgccggt cgtc
//
LOCUS       DASWAV010000107         7821 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U2665
            k141_765045, whole genome shotgun sequence.
ACCESSION   DASWAV010000107 DASWAV010000000
VERSION     DASWAV010000107.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960316
            Sequence Read Archive: SRR10260007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 7821)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7821)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:47:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,929
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7821
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_765045"
                     /isolate="SMAG_U2665"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.36444 N 132.31889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..514)
                     /locus_tag="VF958_02265"
     CDS             complement(<1..514)
                     /locus_tag="VF958_02265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9147964.1"
                     /translation="MTAAAGRTALPARAGRRISAKRTHSSFRMAALGGDVAISLGALF
                     GGYLIRTQIPLPGTDQLLPAGNVRFGVWNVGLVVAVQVFSLAFLGLYGQYERFREPLG
                     RLLLPALFLELLTLASIYFLAPSYSFPRSLVVVYVVLDGVFLAAWRSVLDRLFPLPRR
                     RALVVGGGPAA"
     gene            complement(523..1656)
                     /locus_tag="VF958_02270"
     CDS             complement(523..1656)
                     /locus_tag="VF958_02270"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX9147965.1"
                     /translation="MTAAAIVLAGITLALVLWSYLCYPWLLFRLARRAPVRGEGRSDV
                     GPSVEVLVSAHDEELSIGSRVANLLSQQYAGPLAVCVGCDDCRDRTAVRAREAGDGRV
                     RVVEFAERRGKAAVLNDLIAQSQSEILVFTDANSTFAPDAVRRLVAQFTDPEVGAVCG
                     RLVLDAPDGDAPSPEREFWDRETRLKEAEGRLGVCLGANGAIYAARRDLVKPLPEGSA
                     LDDFLIPARIAVAGASVVFAGNAIAREPAGSDVAAEASRRLRIGIGAGGTLLRERGLF
                     DFRRRPLLALVFLSRKVARWLAPVGALAAILFGLFSPSLRPSAAVLAAALLLLAASVA
                     LRPPISGWAGRLYYFGVINLALAAGVVAGLAGFRRPVWQRTSR"
     gene            complement(1653..2834)
                     /locus_tag="VF958_02275"
     CDS             complement(1653..2834)
                     /locus_tag="VF958_02275"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEX9147966.1"
                     /translation="MKPLLYAVCPRPPHPTRDGSAIRNYHLLAGFAEEFRVRAFVLLP
                     PHLRELAEELPEGIDAERFPQSPRSVRRAAALAASAVGRAYSPFLYRSRPLAIRLRQL
                     ASGEKPGWIVAHSYHVAPLALDAFGPLWIDFHNLDSELWRRMGESAPSRVARAFARSQ
                     APRVSRVEAALVSRAAGISCVSERESDLLARLAPEVPRVVVPNGVDLDRYSYRADPAV
                     EEVIFYVGDLTWPPHAQGMSWFRREVWPLVARTRPEARVEVVGRGAAASRDGRFTFFG
                     EGGDTRPHWSRAAVAVVPLLAAAGTRLKILEAAACGVPVVSTSVGAEGLDFRDGAEIR
                     IADRAEDFADAVSDLLVDPDARRRQAAQARGRVEERYGWKSIGRTFARELRRRLPGAA
                     S"
     gene            complement(2831..3739)
                     /locus_tag="VF958_02280"
     CDS             complement(2831..3739)
                     /locus_tag="VF958_02280"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEX9147967.1"
                     /translation="MSLAAGRRVSAVIVHYLTRKETVESAQALAATAPETEILVVDNA
                     SGDSIEDPLRALVPSARAIRESENRGYGAACNRGARETSREFLMFLNSDAVVQPGAVD
                     ALVAALDRDSHAAVAGPRLENPDGSLQTSILRLPTLGRVFFESAGLAYLTGGRLHGHS
                     ATSEDHARAHEVEAVKGAALLVRRTAFEEVGGFDESFFLYAEESDLAARLRRCGWRIL
                     FEPRARVVHRGGASGGDSLFGQLNESLATYVARHQGKLAGGAARFVLKAGAAARYALS
                     LVTPGERGRIRRSKYRAALAGPPRRA"
     gene            3950..5377
                     /locus_tag="VF958_02285"
     CDS             3950..5377
                     /locus_tag="VF958_02285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9147968.1"
                     /translation="MRKSCLVALAGVLFSSLVFAQAIDIPVSNWTVPPYTASRAGGGL
                     TTMTDFTPPRVFVAVVPCRVVDTRNPNGPYGGPPLPPNFARTFDIDDGPCGIPAGAEA
                     YSLNFGGIAPPADGFLTAWPTGSPQPGVSQLNFLGGEVVANAAIVPAGNNGAIDVLVN
                     VGTHIYIDINGYFSDNLGQTVNPFILNNTSPFPGATIFGNNLSSGYGIRGYSQNAGLN
                     GAGVFGSYGFMSLQENYNPAGIRGQGTSYGTVGISPNEGVTGGLVNFSGFERAFGILG
                     FNAGALASQAYGVYSGGNTGASGTKQFVEPHPTDPTKVIRYISLEGPEAGTYFRGKGK
                     FEGGTARIRVPEDFRMVTDDEGLTVQITPIGGMASVGVMRADLNEIVVQSSRNFEFYY
                     LVNGIRRTQKNLTSPISEGTEFVPRSATSRIPEYLSEGQKQLLIQNGTYRADGTVNME
                     TAHRNGWDRIWAERERPQPAPSPEP"
     gene            complement(5457..7103)
                     /gene="groL"
                     /locus_tag="VF958_02290"
     CDS             complement(5457..7103)
                     /gene="groL"
                     /locus_tag="VF958_02290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018129558.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HEX9147969.1"
                     /translation="MAAKTISYGEEARQRILHGVNKLADAVKVTLGPKGRNVVIEKKF
                     GSPTITKDGVTVAKEIELQDSLENMGAQMVREVASKTSDVAGDGTTTATVLAQAIYRE
                     GIKNVTAGANPMELKRGIDHAVRAAVESLDRLSKPVEGKDIAHVGTISANNDSEIGKI
                     IAEAMDKVGKDGVITVEEAKGLETTLEVVEGMQFDRGYLSPYFITDSERMEAVLENAK
                     VLLFEKKISNMKDLLPILEQIAKQGRPLLIVAEDVEGEALATLVVNKLRGTLQVAAVK
                     APGFGDRRKAMLEDIGILTGGKLISEDLGIKLESVKWEDLGDVKKVTIDKDNTTVLVD
                     DRSKKKQEAIAGRVKQIRTQIEDTTSDYDREKLQERLAKLVGGVAVIKVGASTETEMK
                     EKKARVEDAMHATKAAVEEGIVPGGGVALLRSIDSVEKVVKELSGDEKIGARILIRAL
                     EEPTRQIVSNAGAEGSVVINELKSKGGNWGYNANSEKVEDMLKAGIIDPTKVTKNALQ
                     NAASIAGLMLTTEAMVSEIKEKEKAPAGMPGGGGGMGGMY"
     gene            complement(7126..7416)
                     /locus_tag="VF958_02295"
     CDS             complement(7126..7416)
                     /locus_tag="VF958_02295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013941457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HEX9147970.1"
                     /translation="MKIRPLYDRILVKRVEEKETRRGGIIIPDTAKEKPMEAEVVAVG
                     KGKINEDGKQLPLDVKKGDRILIGKYAGTEIKIDDEEHLILREEEVLAVVEK"
     gene            complement(7567..>7821)
                     /locus_tag="VF958_02300"
     CDS             complement(7567..>7821)
                     /locus_tag="VF958_02300"
                     /inference="COORDINATES: protein motif:HMM:NF025910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX9147971.1"
                     /translation="AGGYQGLGVIYKWRGEYAEALPLFREAAALDPNYRDAWCNLAGA
                     LATLGQSQEALDTLANYRFRHPEDPVAVDLERAIRADASR"
BASE COUNT         1323 a         2616 c         2583 g         1299 t
ORIGIN      
        1 tggcggccgg accgccgccg acgacgagcg cacgcctccg cggaagcggg aagagccggt
       61 ccagcaccga gcgccaggct gcgagaaaaa cgccgtcgag aacgacgtag accacgacga
      121 gcgaacgtgg gaacgagtac gacggagcga gaaagtagat ggaggcgagc gtcaggagct
      181 cgaggaagag agccgggagg agaagccgac cgagcggctc ccggaaacgc tcgtactggc
      241 catagagccc gagaaacgcg agcgagaaca cctgcacggc gacgacgaga ccgacgttcc
      301 agactccgaa gcggacgttg ccggcgggaa gcagctgatc cgtgccggga agcggaatct
      361 gtgtgcggat gaggtacccg ccgaagagtg caccgagcga gatcgccacg tcgccgccga
      421 gcgccgccat ccggaacgag gaatgcgtcc tcttcgccga gatccggcga ccggctcgag
      481 ccggcaatgc ggtgcgtccc gccgccgcgg tcacggcgcc tctcagcggc tcgtccgctg
      541 ccagacaggc ctccggaaac ccgcgagacc ggcgacgaca cctgccgcga gcgccagatt
      601 gatcacgccg aaataataga gcctgccggc ccaccccgag atgggtggcc gaagggcgac
      661 cgaggctgcg agcaggagga gcgccgccgc caggactgcg gcggacggcc gcagggaagg
      721 ggagaaaagt ccgaagagga tcgccgccag ggccccgacg ggcgcgagcc agcgagcgac
      781 cttgcgggag aggaacacga gagcgagaag gggccggcgg cggaagtcga acagacctcg
      841 ctcccggagc agcgtcccgc ccgcgccgat cccgatccgc aaacggcggg atgcctccgc
      901 ggcgacatcg gaaccggcag gctctcgagc gatcgcattg ccggcgaaaa cgacgctcgc
      961 tcccgcgacc gcgattcgcg ccggaatgag gaagtcgtcg agcgccgaac cttccggaag
     1021 cggcttcacg aggtcgcggc gcgcggcgta gatcgcgccg ttcgccccga gacagacgcc
     1081 gagtcggccc tccgcctcct tcagccgcgt ttcgcggtcc cagaactccc gttccggcga
     1141 cggggcatct ccatccggcg cgtcgaggac gagacgtccg cagaccgctc ccacttcggg
     1201 gtcggtgaac tgcgcgacca ggcggcggac cgcgtcgggt gcgaaagtgg agtttgcgtc
     1261 ggtgaagacg aggatttcgc tctgcgattg cgcgatgagg tcgttcagca ctgccgcttt
     1321 cccgcggcgc tccgcgaact cgacgacgcg gacgcgcccg tctcccgcct cccgcgcgcg
     1381 gacggcggtc cggtcgcggc agtcgtcgca gccaacgcaa acggcgagag gtccggcgta
     1441 ctgctgcgag aggagattgg cgacgcgcga gccgatcgac aactcctcgt cgtgggcgga
     1501 cacgagcacc tccaccgacg ggccgacgtc ggatctccct tcacccctca cgggagcgcg
     1561 ccgcgcgaga cgaaagagca gccaggggta gcaaaggtac gaccacagga cgagagcgag
     1621 cgtgatcccc gcgagcacga tcgccgccgc ggtcatgagg cggccccggg aagccgccgg
     1681 cgaagctcgc gcgcgaacgt gcgcccgatc gacttccagc cgtaccgctc ctccactctg
     1741 ccccgcgcct gtgctgcttg ccggcgccgg gcgtccgggt cgacgagaag gtcggaaact
     1801 gcatcggcga aatcctcggc gcggtccgcg atccggatct cggccccgtc gcggaagtcg
     1861 aggccctcag ctccgaccga agtggagacg acgggaactc cgcaggccgc ggcttcgagg
     1921 atcttcagcc tcgttcccgc cgccgcgagg agcggtacga ccgcgacggc ggcgcgagac
     1981 cagtgggggc gtgtatcgcc cccttctccg aagaaagtga agcgtccgtc tctcgaagcc
     2041 gcggctccac gcccgacgac ttcgacccgc gcctcgggcc gcgtccgcgc cacgagaggc
     2101 cagacctcac ggcggaacca cgacattccc tgcgcgtgcg gcggccacgt gaggtcgccg
     2161 acgtagaaga tgacctcttc gacagccgga tccgcgcggt acgagtagcg gtcgagatcg
     2221 acgccgttcg gaacgacgac gcgcggcacc tccggcgcga gccgcgcgag caggtccgac
     2281 tcgcgttcgg agacgcagga gatgcccgcc gcgcgcgaca cgagcgccgc ctctacgcgg
     2341 gagacgcggg gagcctgcga tcgcgcgaac gcccgcgcaa cgcgcgaggg agcgctttcg
     2401 cccatccggc gccagagctc cgagtcgagg ttgtggaagt cgatccagag cggcccgaac
     2461 gcgtcgagtg cgagcggagc gacatggtac gagtgcgcca cgatccagcc gggcttctcg
     2521 cccgaggcga gctggcgcag ccggatcgcg agcggccgcg atcggtagag aaacggcgaa
     2581 taagcgcggc cgaccgcgct ggcggcgagc gccgcggcgc gccggaccga ccgcggtgac
     2641 tgaggaaagc gctcggcatc gatgccctcg ggaagctcct cggccagctc gcgaaggtgc
     2701 ggcgggagga ggacgaaggc ccggacgcgg aactcctcgg cgaacccggc gaggagatgg
     2761 tagttgcgga tcgccgatcc gtcgcgcgtc ggatggggcg gccgggggca gaccgcgtag
     2821 aggagcggct tcatgccctc cgcggcggtc ccgcgagtgc ggcgcggtac ttcgagcggc
     2881 gaatgcgtcc gcgctctccc ggtgtcacga gcgaaagagc gtagcgcgcc gcggccccgg
     2941 ccttgagaac aaaccgagcg gcgcctccgg cgagcttccc ctgatgtcgc gcgacgtacg
     3001 tcgcgaggct ttcgttcagc tgcccaaaga gagaatcacc gcccgaggcg ccgccgcggt
     3061 ggaccacgcg cgctctcggt tcaaagagaa ttcgccagcc acatcgacgg agacgcgccg
     3121 cgaggtcgct ctcttccgca tatagaaaga agctttcgtc gaatcccccg acctcctcga
     3181 acgcggtccg gcggacgagg agcgccgcac ccttgaccgc ttcgacttca tgcgcacgtg
     3241 catggtcttc ggacgtcgcc gagtggccgt gcagccgccc gcccgtgaga taggcgagtc
     3301 ccgcgctctc gaagaagacc cgtccgagcg tcgggagccg caggatcgaa gtctggagac
     3361 tcccgtccgg attctcgagc cggggtccgg ccaccgcggc atgggagtcc cggtcgaggg
     3421 ccgcgaccag ggcgtccacg gcacccggct gaacgaccgc gtcggagttc aggaacatca
     3481 ggaattcccg gctcgtctct cgcgcgcccc ggttgcacgc cgcgccgtag ccgcggttct
     3541 cggattcgcg gatagcacgg gccgaaggca ccaacgcccg gagcggatcc tcgatggagt
     3601 ctcccgacgc gttgtcgacg acgaggatct ccgtctccgg cgcggtcgcg gcaagggcct
     3661 gagccgattc gaccgtctcc ttgcgggtga gatagtggac gatgacggca gagacacgac
     3721 gtccggccgc aaggctcatt ccgaacggca gtatagaggt cgaggagcct ttccttcgtt
     3781 caatcgggcg ggataggatt cccggcgtcc cggcctgtgg cgagaacgtg ggggtgcgct
     3841 cttgcgtcga ggctacgaat tcgtggtgcc aagtctatcg agccggccgc tcgtgccgac
     3901 tcgtttcatt acttcaaccg cccgtcggtt taccaaaacg gaggtgatca tgaggaagtc
     3961 ctgtctcgtc gcactcgccg gcgtcctttt ttcctcgtta gtcttcgcgc aagccatcga
     4021 catccccgtc tcgaactgga cggtgccgcc ttacaccgcc tcgcgcgctg ggggtggcct
     4081 gacgacgatg accgacttca cgccgccgag agttttcgtc gcggtcgtgc cgtgccgggt
     4141 ggtcgacaca cgcaacccga acggtcccta cggcggtccg ccgctcccac cgaacttcgc
     4201 gcgcacgttc gacatcgacg acggaccgtg cgggattccg gcgggagccg aggcatactc
     4261 tctcaacttc ggcgggatcg cgcccccggc cgacgggttc ctgacagcat ggccgaccgg
     4321 gagtccgcag cccggggtct cccagctgaa cttcctgggc ggggaggtcg tggcgaatgc
     4381 cgcgatcgtc ccggcgggga acaacggcgc gatcgacgtt ctcgtcaacg tcggaacaca
     4441 tatttacatc gacatcaacg ggtacttcag cgacaacctc ggtcaaacgg tgaacccttt
     4501 cattctgaac aacacttcgc ctttcccggg ggccacgatt ttcggaaaca acctgagcag
     4561 tggatacggg attcgcggtt actcccagaa cgccggcttg aacggcgccg gggtgttcgg
     4621 ctcctacgga ttcatgtctc tgcaggagaa ttacaacccg gccgggatac gaggccaggg
     4681 gaccagctac ggcaccgtgg gcatctcccc gaacgagggc gtgacgggtg gtctcgtcaa
     4741 cttctcgggc ttcgaacgcg catttggcat tctcggcttc aacgcaggag cgttggccag
     4801 tcaggcctac ggggtctatt ccggaggaaa caccggggcg agcggcacca agcagttcgt
     4861 cgagccgcac ccgaccgacc cgacgaaggt catccggtac atctctctcg agggcccgga
     4921 agccggcacc tacttccggg gcaaaggcaa gttcgagggc gggacggcac gaatccgggt
     4981 cccggaggac ttccggatgg tgacggatga cgagggactg acggttcaga tcacgccgat
     5041 cggcggaatg gcgtcggtcg gggtcatgag ggccgacctc aacgagatcg tcgtgcagtc
     5101 gtcgcggaac ttcgagttct attacctggt caacgggatc cggcgcactc agaagaacct
     5161 gacctcgccg atcagcgagg gaaccgagtt cgtgccgcgg tccgccacgt cgaggatacc
     5221 ggagtacctg agcgagggtc agaaacagtt gctcatccag aacgggacct accgggccga
     5281 cggtaccgtg aacatggaga cggctcatcg caacggatgg gatcggatct gggccgagcg
     5341 cgaacgtccg cagcccgcgc cgtcaccgga accctagacc ccgacggacc cagacaagaa
     5401 aaagccccgg cgcgagccgg ggcttttgtt ttccgtctta cgtttccgtt ccgggatcag
     5461 tacattccgc ccattccgcc gccgccgccg ggcattccgg ccggcgcttt ctctttctcc
     5521 ttgatctcgg agaccatcgc ctcggtcgtc agcatgaggc cagcgatcga cgcggcgttc
     5581 tggagagcgt tcttggtgac cttggtcgga tcgatgatgc ccgccttcag catgtcttcg
     5641 accttctcgc tgttggcgtt gtagccccag ttgccgccct tggacttgag ctcgttgatg
     5701 acgacggagc cttccgcacc ggcgttggac acgatctgcc gggtgggctc ctcgagagcg
     5761 cgaatcagga tgcgggcgcc gatcttctcg tcgcccgaaa gttccttgac gaccttctcg
     5821 accgaatcga tgcttcgaag gagcgccact ccgccgccgg ggacgatccc ctcttcgacg
     5881 gccgccttgg tggcgtgcat ggcatcttcc acgcgcgcct tcttctcctt catctcggtc
     5941 tcagtcgagg ctccgacctt gatgaccgcg acgccgccga cgagcttcgc gagacgctcc
     6001 tggagcttct cgcggtcgta gtccgacgtg gtgtcctcga tctgcgtgcg gatctgcttg
     6061 acgcggccgg cgatcgcttc ctgctttttc ttggagcggt cgtcgacgag cacggtcgtg
     6121 ttgtccttgt cgatcgtcac cttcttcacg tcgcccaggt cttcccactt gacgctctcg
     6181 agcttgatgc cgagatcctc ggagatgagc tttccgcccg tcaagatgcc gatgtcctcg
     6241 agcatcgcct tgcggcgatc accaaagccc ggcgccttga cagccgcgac ctggagcgtg
     6301 ccccgcagct tgttgacgac gagcgtcgcg agcgcctcgc cctcgacgtc ctcggcgacg
     6361 atgagcagcg gccggccctg cttcgcgatc tgctcgagga tcgggaggag atccttcatg
     6421 ttcgagatct ttttctcgaa gagaaggacc ttcgcgttct cgagcaccgc ctccatccgc
     6481 tcggagtcgg tgatgaagta gggggacagg tacccgcggt cgaactgcat accctcgacc
     6541 acttcgagcg tcgtctcgag ccccttcgcc tcctcgaccg tgatcactcc gtccttgccg
     6601 accttgtcca tggcctcggc gatgatcttc ccgatctccg agtcgttgtt cgccgagatg
     6661 gttccgacgt gggcgatgtc tttgccctcg acgggcttcg agagtctgtc gagagactcg
     6721 acggcggccc ggaccgcatg gtcgattccg cgcttcaact ccatcgggtt cgcgcccgcg
     6781 gtgacgttct tgattccctc gcgatagatc gcctgcgcga gaaccgtcgc ggtcgtcgtg
     6841 ccgtccccgg cgacgtcgga ggtcttcgag gcgacctcgc gcaccatctg cgcgcccatg
     6901 ttttcgagcg aatcctggag ctcgatctcc ttcgccaccg tcacgccgtc cttggtgatc
     6961 gtgggcgatc cgaacttctt ctcgatgacg acgttgcggc ctttcgggcc gagcgtcacc
     7021 ttgacggcgt cggcgagctt gttgacgccg tggaggatgc gctgtcgcgc ctcctcgcca
     7081 tatgaaatcg tcttcgctgc catctcttcg tttctccttc cgggtttact tctcgaccac
     7141 ggcgaggacc tcttcttcac ggagaatgag gtgctcctcg tcgtcgatct tgatttcggt
     7201 tccggcgtac ttgccgatga ggatgcggtc gcccttcttc acgtcgagcg gaagctgctt
     7261 gccgtcctcg ttgatctttc ccttaccgac ggcgacgacc tcggcctcca tcggcttttc
     7321 cttggcagtg tcggggatga tgatgccccc gcgacgcgtc tctttctcct cgacgcgctt
     7381 cacgaggatt cggtcataga gcggacgaat cttcattcct tctcctcctc tggtggtttg
     7441 gatgccccgg aacaacggat ccggggcgga tggcgatgtt agcactcctc ggtgacgagt
     7501 gctagcaccc gtgaggggcg ctgccgcgcc gacaggcgca gggccaaact actgacgtag
     7561 cgcgacttac cttgacgcgt ccgcccggat cgcccgttcc aggtcgacgg ccaccggatc
     7621 ctcggggtga cggaagcggt agttcgcgag ggtgtccagc gcttcctgtg actgcccgag
     7681 agtcgcgaga gcgcccgcca ggttgcacca cgcgtcgcgg tagttcggat cgagggcggc
     7741 cgcttcccgg aagaggggga gcgcctcggc atactcccct ctccacttgt agatcacgcc
     7801 cagtccctgg tagccgccgg c
//
LOCUS       DASWAV010000108         1912 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U2665
            k141_325098, whole genome shotgun sequence.
ACCESSION   DASWAV010000108 DASWAV010000000
VERSION     DASWAV010000108.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960316
            Sequence Read Archive: SRR10260007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 1912)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1912)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:47:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,929
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1912
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_325098"
                     /isolate="SMAG_U2665"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.36444 N 132.31889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..540)
                     /locus_tag="VF958_02305"
     CDS             complement(<1..540)
                     /locus_tag="VF958_02305"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011322404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HEX9147972.1"
                     /translation="MFTGIVTAIGRVSALETRRGGGTRLTVLPPPRYGGFRRGESVAV
                     SGVCVTSITTGRLLVADLSGETLRVTTLGSLEGGDAVNLERALEWRGRVAGHFVLGHV
                     DAVARVLAIESARGSWTYRFSIPAGLGRFVARKGSVALDGVSLTVAARRARDFDVAVI
                     PETRRVTTLGSKRAGDPVNF"
     gene            complement(568..1674)
                     /gene="ribD"
                     /locus_tag="VF958_02310"
     CDS             complement(568..1674)
                     /gene="ribD"
                     /locus_tag="VF958_02310"
                     /EC_number="1.1.1.193"
                     /EC_number="3.5.4.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012097533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA];
                     GO_function: GO:0008835 -
                     diaminohydroxyphosphoribosylaminopyrimidine deaminase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HEX9147973.1"
                     /translation="MNDREDAFLSRALDLAERGRYTVAPNPMVGAIVARGGRIVAEAW
                     HRRAGGAHAEAAALDRAGPRARGADLYLTLEPCVHSGRTPPCAPRVVESGVRRVFLAS
                     RDANPLVAGRGVAALRRAGIEIVQARGELRRRAERQNEKFRLWISRRRPFVLAKWAAT
                     LDGKTASGTGESRWITGGEARRRGLLLREEYDAVLVGSGTVIADDPLLTRRLGTAGDR
                     LHRRIVLDGRLRAPASARLFRDPEGLLVVTTLRPDHPRARRLSERGVEVWSSPVRGGD
                     VSLRPLVARLAGAGITSLMVEGGAETLWRFFRAGLVDAVAVFTAPRVLGGGRAPGGVG
                     GRGFPLSRAPKIVDVRHERVGEDWLVTGRVERRE"
     gene            complement(1671..>1912)
                     /locus_tag="VF958_02315"
     CDS             complement(1671..>1912)
                     /locus_tag="VF958_02315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004012927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HEX9147974.1"
                     /translation="AQARRFVEAAGATGVVLTKLDGSARGGIVLAIYRELSLPVLFVG
                     VGEGVDDLVPFDPSDYSRALLGDAAPEEAPESSPA"
BASE COUNT          290 a          730 c          665 g          227 t
ORIGIN      
        1 gaagttgacg ggatcgcccg cgcgcttcga accgagcgtc gtcacgcgcc gggtctcggg
       61 aatcaccgcg acgtcgaagt cgcgggcgcg ccgcgcggcg accgtgaggc tcacgccgtc
      121 gagcgcgacc gagcccttcc gtgcaacgaa ccggccgagg cccgccggga tcgagaaccg
      181 gtaggtccac gatccccgcg cgctctcgat cgcgagcacg cgcgccaccg cgtcgacgtg
      241 cccgagcacg aagtgccccg cgacccggcc ccgccactcg agagctcgct cgaggttgac
      301 cgcgtcgccg ccttcgagtg aaccgagagt cgtcacccgg agggtctctc cggagaggtc
      361 cgccacgagg agccgtccgg tcgtgatcga cgtcacgcag acgcccgaga cggcgacgct
      421 ctcgccgcgg cgaaatccgc cgtagcgcgg cggagggaga acggtcagcc gcgtgccgcc
      481 gccgcgccgc gtctcgagcg cggacacgcg gccgatggcg gtcacgattc cggtgaacat
      541 ccgtgtgagt gtaccgatcc ggagcggtta ctctctacgt tcgacccgcc cggtcacgag
      601 ccaatcctcc ccgacccgtt cgtgccggac gtccacgatc tttggcgccc gcgaaagggg
      661 gaagcctcgc ccgccgacgc cgcccggcgc ccgtcctccg ccgagcacgc ggggagccgt
      721 gaagacggcg accgcgtcga ccagtcccgc gcggaagaag cgccacagcg tctcggcgcc
      781 cccctcgacc atcaggctcg tgatccccgc acccgcgagc cgcgcgacca gtggccgaag
      841 gctgacgtcc cccccgcgaa ccggcgagct ccagacctcg acgccgcgct ccgacaggcg
      901 cctcgcgcgg ggatggtccg gccgcagggt cgtgaccacg agaaggcctt ccgggtcccg
      961 gaagagccga gccgaggcgg gagcacgcag ccggccgtcg agaacgatcc gccggtggag
     1021 acggtccccg gcagtcccga gtcgccgcgt cagaaggggg tcgtccgcga tcaccgtccc
     1081 cgatccgacg agaactgcgt cgtactcctc ccggagaagc agtccgcggc ggcgcgcctc
     1141 gcccccggtg atccagcgac tctcgcccgt accggacgcg gtcttgccgt cgagggtcgc
     1201 ggcccatttt gcgagaacga acggccgccg cctcgaaatc cagagtcgga acttctcgtt
     1261 ctgcctctcc gcgcggcggc ggagttcgcc cctggcctgg acgatctcga tgccggcgcg
     1321 gcgaagggcg gcgacgccgc ggccggcgac cagcgggttt gcgtcccgcg acgccaggaa
     1381 gacgcgccgg acacccgact cgacgaccct cggcgcgcac ggcggcgtcc ggccggaatg
     1441 gacgcacggc tcgagcgtca ggtacaggtc ggcgccgcgc gcgcgggggc cggcgcgatc
     1501 gagcgctgcg gcctcggcgt gcgcgcctcc cgcgcgccga tgccacgcct ccgcgacgat
     1561 ccgaccgccc cgcgctacga tcgcgcccac catcgggttc ggcgcaaccg tgtaccggcc
     1621 gcgctccgcg aggtcgagcg cgcgagacag gaacgcgtcc tcccgatcgt tcacgccggt
     1681 gagctctccg gggcttcctc gggggcggcg tctccgagga gcgcgcggga atagtcggaa
     1741 ggatcgaaag gaacgagatc gtcgacgcct tcgccgaccc cgacgaacag aaccggaagc
     1801 gaaagctcgc ggtagatggc caggacgatc ccgccccggg cgctgccgtc gagcttcgtc
     1861 aggacgaccc ctgtcgcccc tgccgcctcg acgaagcgtc gcgcctgcgc ga
//
LOCUS       DASWAV010000109         1910 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U2665
            k141_86601, whole genome shotgun sequence.
ACCESSION   DASWAV010000109 DASWAV010000000
VERSION     DASWAV010000109.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960316
            Sequence Read Archive: SRR10260007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 1910)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1910)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:47:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,929
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1910
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_86601"
                     /isolate="SMAG_U2665"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.36444 N 132.31889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(385..951)
                     /locus_tag="VF958_02320"
     CDS             complement(385..951)
                     /locus_tag="VF958_02320"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020251259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004797 - thymidine kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HEX9147975.1"
                     /translation="MALVKTHLGWIEVICGSMFSGKSEELIRRLRRAIIAKQRVQIFK
                     PTIDSRYSDAEIVSHSEMRMPSVSVASSAELLDKVDHRTEVIGIDEAQFFDEGVVEAA
                     QRLADQGKRVIVAGLDKDFRGLPFGPIPALMAVAEDVTKTLAICVRCGSPANNTQRLV
                     ESDELVVVGAQGVYEARCRRCFEPPSRP"
     gene            complement(964..1446)
                     /locus_tag="VF958_02325"
     CDS             complement(964..1446)
                     /locus_tag="VF958_02325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9147976.1"
                     /translation="MRVYLSGAIEHASDGGKGWRKELARLLREELHHDVYDPAADEKK
                     SLTDDELANFRRWKLEDPMRFRETVRKIIAWDLDKIEHEADFVIARWDRAAGRGGGTA
                     AEITLAHRLGKPVFLLLAMPTREASGWVLSAATEVFETLDALKHRLRELFARTHPSLR
                     "
     gene            complement(1443..>1910)
                     /gene="nth"
                     /locus_tag="VF958_02330"
     CDS             complement(1443..>1910)
                     /gene="nth"
                     /locus_tag="VF958_02330"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0140078 - class I DNA-(apurinic or
                     apyrimidinic site) endonuclease activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HEX9147977.1"
                     /translation="DIRQTGFFNSKAKSLRRAGAAIAAEHGGRVPETMEDLVRLPGVG
                     RKTANVILGNAFGKDEGFVVDTHVGRVSRRLGFTREADPVRIEDELNRLVPKGRRTLA
                     AHQLIFHGRQICVARRPRCEICPVNLLCPKVGVPLAIRRAASSPVSRLPSSAR"
BASE COUNT          310 a          628 c          649 g          323 t
ORIGIN      
        1 ggccgaggcg acgctcggcg aaatcgtctc gagcctgaag accgtctacg gcgagcaccg
       61 cccagcgtag gcacggggac gggtcccggg gacctgcacc cgccgcagtc cacttaagtc
      121 caagcgccaa aagcgcttgt cgatgtggaa aaaaattgaa cgctccggtt cgcaccgtcg
      181 tacgaccggc gcaagtccgt tggaatcacg taattagctg atggcaaatg gggttgtcct
      241 atcgcacagg actcggtgca atccggagac gagccgcgcc gcggcgcgct tttcgggagc
      301 tttccgcagg gatttcgaca gccttttccg ctgcggtttc ggaaaactga tttgtcgtcg
      361 atttttgcga cggtttctgc ggggtcaggg ccgcgagggc ggctcgaagc accggcggca
      421 gcgcgcctcg tacaccccct gggcgccgac gacgacgagc tcatcgctct cgaccagccg
      481 ctgggtgttg ttggcgggcg agccgcaccg cacgcagatg gcgagcgtct tggtcacgtc
      541 ctcggcgacc gccatgagtg cggggattgg gccgaagggc aggccgcgaa agtccttgtc
      601 gagccccgcg acgatgaccc gcttgccctg atcggccagg cgctgcgcag cctcgacgac
      661 tccttcgtcg aaaaactgcg cctcgtcgat cccgatgacc tcggtgcggt ggtcgacttt
      721 gtccaggagc tccgcgctcg aggcaacgga gaccgagggc atccgcatct cgctgtgaga
      781 gacgatctcg gcgtcggagt accgcgagtc gatcgtcggc ttgaagatct ggacgcgctg
      841 cttcgcgatg atcgcccggc gcagccgccg gatgagctcc tcgctcttgc cggagaacat
      901 gctcccgcag atgacttcga tccaaccgag atgcgtcttg accagggcca tgggccgccg
      961 atcttatcgg agcgaaggat gcgtcctggc gaacagctcc cggagccggt gcttcagggc
     1021 gtccagggtc tcgaagacct ccgtcgcggc ggagaggacc cagcccgagg cttcgcgggt
     1081 cggcatcgcc agcagcagga agaccggctt cccgagccgg tgggcgaggg tgatctcggc
     1141 ggcagtgccg ccgccccggc cggccgcgcg gtcccaccgg gcgatcacga agtccgcctc
     1201 gtgctcgatc ttgtcgagat cccacgcgat gatctttcgc acggtctccc ggaagcgcat
     1261 cgggtcctcg agcttccagc ggcggaagtt cgcgagctca tcatcggtca ggctcttctt
     1321 ctcgtcggcc gccgggtcgt agacgtcgtg gtggagctcc tcgcgaagga ggcgagccag
     1381 ctctttccgc cagcccttcc cgccgtcgga agcatgctcg attgcgcccg agagatagac
     1441 cctcatcggg cggaagacgg caggcgggag acgggagacg aggcggcccg ccggatcgcg
     1501 agcggcacgc cgaccttcgg gcagaggagg ttcaccgggc agatctcgca tcgcggccgt
     1561 cgcgcgacgc agatctgccg gccgtggaag atcagctggt gggccgcgag ggtgcgccgc
     1621 cctttcggca cgaggcggtt caactcgtcc tcgattcgga cggggtcggc ttcgcgggtg
     1681 aagccgagcc ggcgcgagac gcgcccgacg tgggtgtcga ccacaaaacc ctcgtctttg
     1741 ccgaaagcgt tgccgaggat cacgttcgcc gtcttgcggc cgactcccgg cagtcgaacg
     1801 agatcctcca tcgtctcggg aacccgtccg ccgtgctccg cggcgatggc cgcgcccgcg
     1861 cgccgcaggc tcttcgcctt cgaattgaaa aagccggtct gccggatgtc
//
LOCUS       DASWAV010000110         2704 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U2665
            k141_366426, whole genome shotgun sequence.
ACCESSION   DASWAV010000110 DASWAV010000000
VERSION     DASWAV010000110.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960316
            Sequence Read Archive: SRR10260007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2704)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2704)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:47:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,929
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2704
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_366426"
                     /isolate="SMAG_U2665"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.36444 N 132.31889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..600
                     /gene="cmk"
                     /locus_tag="VF958_02335"
     CDS             <1..600
                     /gene="cmk"
                     /locus_tag="VF958_02335"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: protein motif:HMM:NF014300.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004127 - cytidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HEX9147978.1"
                     /translation="LGVPYIDTGAMYRAVGLAARDRGLTLPLSDPERVSEIAERISIE
                     MDAFEGATRIRLDGRDVSDVIRLPEISLYASAVSAIPAVRRRLVGEQRRMAAERGGVL
                     EGRDIGTRVFPDTPHKFFLTAPLAVRAERRARELALRGTPQPVEAVRAEIERRDHADS
                     TRADSPLTLDPTYTVVDTGGRGVEEIIEDLVARVGARSA"
     gene            764..2527
                     /locus_tag="VF958_02340"
     CDS             764..2527
                     /locus_tag="VF958_02340"
                     /inference="COORDINATES: protein motif:HMM:TIGR00717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HEX9147979.1"
                     /translation="MQTEEELSPQEYEHLLAQYENSFKNLQEGQIIRGRVLAITPSEV
                     IVDIGYKSEGIIALAEFTDFSGNVTVHEGDSVDVLLERTEDQNGYVVLSKDKAEKMKV
                     WDEVERSYRSGSTVRGRVIDRIKGGLAVDIGVKAFLPGSLVDVKPVKNLESLRGKELD
                     FKVISVDKKRGNIVLSRKAVVEIEQEARKRETLQLLEEGRVLRGTVKNLTDYGAFVDL
                     GGLDGLLHVTDMSWGRVNHPSDLVKVGDEIDVVVLKFDRETERVSLGTKQLTEDPWVH
                     VPGKYPAGSRVSGRVTNVTDYGAFVELEEGVEGLVHVSEMSWSKKVKNPSKLVSPGDQ
                     VEAVVSDVNPDARRISLSLKDTLPDPWETVLQKYAIGSRVHGKVRNLTDFGAFVEIEE
                     GIDGLVHVSDMSWTKRIKHPSEVLKKGDEVEAIITSIDEENRRISLSIKEFQPNEFQT
                     FRESHQPGDVVDGVVTRIADFGVFVQIGGLVEGLMHVSETALPRGEKPQDHFHEGNPI
                     RVRILRIDEAEMKIGLSGVDEGGQPLGAPEASAVAAEEITSEPEAESPGPAVADTAEG
                     IEGEVAPKKRRVRKKAEEPKA"
     gene            2576..>2704
                     /locus_tag="VF958_02345"
     CDS             2576..>2704
                     /locus_tag="VF958_02345"
                     /inference="COORDINATES: protein motif:HMM:NF012442.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0030527 - structural constituent of
                     chromatin [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HEX9147980.1"
                     /translation="MTKAELIERVAVATDVTKKQAEGIVDTIFEAIIRSLKQGQKIE"
BASE COUNT          512 a          850 c          928 g          414 t
ORIGIN      
        1 ctcggcgtcc cgtacatcga caccggcgcg atgtatcgcg ccgtcggcct ggcagctcgg
       61 gaccgcgggc tcactcttcc cctctcggac ccggagaggg tctcggagat cgcggaaagg
      121 atctcgatcg agatggacgc cttcgaaggg gcgacccgca tccgcctcga cggccgcgac
      181 gtgtccgacg tgatccgact tcccgagatc tcgctctacg cgtcggcggt ctctgcgatt
      241 ccggcggtgc gccgtcggct cgtcggcgag cagcgccgca tggcagccga gcgggggggc
      301 gtgctcgagg gaagggacat cgggacccgg gtctttcccg acacgccgca caagttcttc
      361 ctgaccgcgc ccctcgcggt gcgggccgaa cggcgggccc gggagctcgc cctccggggg
      421 accccgcagc cggtcgaagc ggtccgggcg gaaatcgagc ggcgcgacca cgccgattca
      481 acccgcgccg attcgcccct gaccctcgac ccgacgtata ccgtcgtgga cacaggcggg
      541 cgcggcgtgg aggagatcat cgaggacctc gtggcgcggg tgggagcgcg gtcggcgtga
      601 cggccggggt ggggtccggt ccgcaggacc tgacccccgc ttcgcaccgg gttgactgac
      661 ctcgatgctg caaagtatat ttcgcgtttt cagagactta tgagggggtc atccctgttc
      721 catgcctgct gaaaatcaca aagggggtcg tcagaccgaa ccgatgcaga ccgaagaaga
      781 actctcgccg caggaatacg agcacctgct ggcccagtac gagaactcgt tcaagaacct
      841 ccaggagggt cagatcatcc ggggccgcgt gcttgcgatc acgccctcgg aggtcatcgt
      901 cgacatcggc tacaagtccg aagggatcat cgcgttagcc gagttcaccg acttttccgg
      961 caacgtcacc gttcacgagg gggactcggt cgacgtcctc ctcgagagga cagaggacca
     1021 aaacggctac gtcgtcctct ccaaggacaa ggccgagaag atgaaggtct gggacgaggt
     1081 cgaacggtcc taccgctcgg gctcgaccgt ccgcggacgg gtgatcgacc ggatcaaggg
     1141 ggggctcgcc gtcgacatcg gagtcaaggc tttcctgccg ggttcgctcg tggacgtgaa
     1201 accggtcaag aacctcgagt cgctccgggg caaggagctc gacttcaagg taatttccgt
     1261 cgacaagaag cgcggcaaca tcgttctgtc gcgcaaagcc gtcgtcgaga tcgagcagga
     1321 ggcccgaaag cgggagaccc tgcagctgct cgaggagggt cgggtcctgc gcggaaccgt
     1381 gaagaacctg accgactacg gcgcgttcgt ggatctgggc gggctcgacg gcctcctcca
     1441 cgtcaccgac atgtcctggg ggcgcgtgaa ccacccgtcc gacctcgtca aggtcggcga
     1501 cgagatcgac gtcgtcgtcc tgaagttcga ccgcgaaacc gaacgcgtct cgctcggcac
     1561 gaagcagttg accgaggacc cgtgggtgca cgtccccggc aaatatcccg cgggctcgcg
     1621 ggtttcgggc cgcgtcacga acgtgacgga ctacggcgcg ttcgtcgagc tcgaggaggg
     1681 cgtcgagggg ctcgtccacg tctcggagat gtcctggtcg aagaaggtga agaacccttc
     1741 gaagctggtc tctccggggg accaggtcga ggccgtcgtc tccgacgtca acccggacgc
     1801 gcggcggatc tcgctctcgc tcaaagacac ccttcccgac ccctgggaaa cggttctcca
     1861 gaagtacgcg atcggcagcc gcgtgcacgg caaggtgcgc aacctgaccg acttcggcgc
     1921 gttcgtcgag atcgaagagg gtatcgatgg gctcgtgcac gtgtcggaca tgtcctggac
     1981 caagcgcatc aagcacccct ccgaagtcct gaagaagggc gacgaggtcg aggcaatcat
     2041 cacctcgatc gacgaggaga accggcggat ctcactctcg atcaaggaat ttcagccgaa
     2101 cgagtttcag accttccggg agagccacca gccgggcgac gtcgtggacg gcgtcgtgac
     2161 ccgcatcgcc gacttcggcg tgttcgtcca gatcggagga ctcgtcgagg ggctgatgca
     2221 cgtgtccgag acggcgctgc cgcgcggaga gaagccccag gatcacttcc acgagggcaa
     2281 cccgatccgt gtacggatcc tccggatcga cgaggccgaa atgaagatcg ggctctcggg
     2341 ggtcgacgag gggggacaac cgctcggcgc cccggaggct tccgcggttg cggccgaaga
     2401 gatcacctcc gagccggagg cggaatctcc cggtccggcc gtggccgaca cggccgaggg
     2461 catcgagggg gaggttgccc cgaagaaacg ccgcgtccgg aagaaggcgg aagagcccaa
     2521 ggcttaatcg ggcgggatct ccccgcaagg ggccggatcc cgcgagggag tcgcgatgac
     2581 gaaggccgaa ctgatcgagc gcgtggcggt cgcgacggac gtcacgaaga agcaggccga
     2641 ggggatcgtg gatacgatct tcgaggcgat catccggtcc ctcaagcagg ggcagaagat
     2701 cgag
//
LOCUS       DASWAV010000111         4494 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U2665
            k141_366479, whole genome shotgun sequence.
ACCESSION   DASWAV010000111 DASWAV010000000
VERSION     DASWAV010000111.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960316
            Sequence Read Archive: SRR10260007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 4494)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4494)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:47:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,929
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4494
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_366479"
                     /isolate="SMAG_U2665"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.36444 N 132.31889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1396
                     /locus_tag="VF958_02350"
     CDS             <1..1396
                     /locus_tag="VF958_02350"
                     /inference="COORDINATES: protein motif:HMM:NF014798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transketolase C-terminal domain-containing
                     protein"
                     /protein_id="HEX9147981.1"
                     /translation="VCMRICGHAHHDDMLYLGKEPPISWSYPPLTDAGYADRALYEYW
                     SKRDPIPTYAARLEAEGVIRDGELDRMKAEAEIFVEQEARHVIAAPWPEPDVAGRGVF
                     AGEPSRRKVEVLDPGERTRRTETPVLAVEPAPPFDPRGKTFLEAVMLGVRDALASDPR
                     VFVYGEDVGGKYGNAFLLLRPLLAEFGDRIVNSPLSEAAVLGVCVGAALAGQRPIGEI
                     QFNDFVATGFNQLVNNAAKIRYRWGGSVPMVVRMPWGGLRHAGPYHSQNTEPWFYRTP
                     GLKIVAPSTPADARALLVSAVADPDPVLYYEHIALYRDPRIKQLLTEEPPAPLPLGKA
                     ALRRDGDDLVIVSYGAYVHVALRVAEKLSQDGVEASVLDLRSLVPLDREALLALARRC
                     GRVLIVHEDSRTGGIGESLAAIVQEEAFEYLDAPVRIVGALDTPVPYSPPLEEAFLPS
                     EAEVERAARLLLAY"
     gene            complement(1390..2586)
                     /locus_tag="VF958_02355"
     CDS             complement(1390..2586)
                     /locus_tag="VF958_02355"
                     /inference="COORDINATES: protein motif:HMM:NF024241.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX9147982.1"
                     /translation="MNVWKSLRRLPKGVWVISGAFLVNRAGSMILAFLVLYATRDLGL
                     TAERAGLAVTAFGLGSLLAAPFAGRLADRFPPLSIMQTSLVVGGLLAVLLPTARAFVP
                     LVAGVLLWASVSEAYRAPSLAIIGEITPPEQLKPAFSLVRLAANLGLSIGPVIGGLLA
                     ERSFPAIFVVDGTTSVLAGIILIVLAGRMGLPKRTVRVGDDVRPPWAALRDRDFVLFV
                     AAMVPILAVVFQCLSAMSLYVVRDLGVPPSGYGLLLAINTVLIVILDLPINAATARWP
                     HRRALLFGSALIAFGFGALAFARTPVAVAATIVVWTFGEIFTFGALNALAVELAPPAR
                     RGEYMGLYQMAFSLAFVAGPALGVFVLERLGPAALWSGCFAVALLSGAFLAKVRSPEP
                     RTVSFQ"
     gene            2639..>4494
                     /locus_tag="VF958_02360"
     CDS             2639..>4494
                     /locus_tag="VF958_02360"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HEX9147983.1"
                     /translation="MMFAPGSKHGPFEILGPLGSGGMGAVYRARDSRLHRDVAIKALP
                     PDFASDPERVARLSREARLLASLNHPNIAAIYGLEEADGSPFLVMECVEGDSLAQKLA
                     AGPLPVDDALAIGTQIAAGLEAAHGAGVIHRDLKPANVMIRGDGSVKILDLGLARSVE
                     TSTAPSDPSLSPTIPSGGTATGVILGTAAYMSPEQARGRALDKRTDVFSFGCVLYECL
                     TGRRAFPGETISDTLAAILRGEPDWEALPPDTPEQVRRVLHRCLQKDPRKRLHDIADA
                     RIDLEEAISSPHAFETATPVVARSRRGPALLWALAGALIGIVAVYSVGRLFGRPASAP
                     SKPVRAVLPLPPNTRLGFAGRPAIAVSPDSRTVVFGAVEGGIPRLYRRDLGGSEATAI
                     AGTEGGFAPFISPDGEWLGFFTPIEMKKVPLAGGSPIRISLVPPVTAGGAWDADGTIV
                     FTLTANGPLSRIPDAGGKIQAVSALDNSRGEHSHLWPQILPDRRGILVTMVVGRDFQD
                     FDNAQIVVLDPKTGHRTVVLEGSPFARYAAGQIFFVRGGSMFRAPFDLSRLSVTGPPV
                     PLAERVTIESGNGTASFAITNDGTLVYADGPQVPPVQTVVLQLDRRGIEKPLP"
BASE COUNT          745 a         1543 c         1528 g          678 t
ORIGIN      
        1 cgtctgcatg cgcatctgcg ggcacgcgca ccacgacgac atgctgtacc tcggcaagga
       61 gccgccgatc tcctggagct acccgccgct gacggatgcc ggctacgccg accgtgcgct
      121 ttacgagtac tggtcgaagc gtgatccgat cccgacctac gccgcccgtc tcgaggcgga
      181 gggggtcatc cgcgacgggg aactcgaccg catgaaagcc gaggcggaga tattcgtcga
      241 gcaggaggcg cgacacgtga tcgccgcgcc gtggcccgag ccggatgtcg cgggccgggg
      301 ggtcttcgcg ggagagccgt cgcggcgcaa ggtggaagtg ctcgatcccg gagagcggac
      361 gcgccggacc gagacccctg ttctcgcggt cgagccggcg ccgccgttcg atccgagagg
      421 gaagacgttt ctcgaagcgg tcatgctcgg cgtgcgcgac gcgctcgcgt ccgacccgcg
      481 cgtcttcgtc tacggcgagg acgtcggcgg caaatatggc aacgcattcc tgctcctgcg
      541 gccgctgctc gccgagttcg gagaccgcat cgtcaactct ccgctctcgg aagccgccgt
      601 gctcggcgtg tgcgtcggcg ccgccctcgc ggggcagcgc ccgatcggag agatccagtt
      661 caacgacttc gtcgcgacgg gattcaacca gctcgtcaac aatgccgcaa aaatccgcta
      721 ccgctggggc ggctcggtgc cgatggtcgt gcggatgccg tggggcggcc tgcgccacgc
      781 cgggccgtac cacagccaga acacggagcc ctggttctac cggacgccgg gcctgaagat
      841 cgtcgccccc tcgactcccg ccgacgcgcg cgctctcctc gtatccgccg tcgccgatcc
      901 ggatccggtc ctctactacg agcacatcgc gctctaccgc gatccgcgga tcaagcagct
      961 cctcaccgag gagccgccgg cgccgctccc gctcgggaaa gctgccctcc gacgggacgg
     1021 cgacgacctc gtgatcgtgt cgtacggggc gtacgttcac gtcgcgcttc gggtcgccga
     1081 gaagctctcg caggacggcg tcgaggcctc cgtccttgac ctgaggagcc tcgtgccgct
     1141 cgatcgcgaa gctcttctcg cgctcgcgcg gcgttgcggc cgcgtcctca tcgtgcacga
     1201 agactcacgc acgggcggca tcggcgaaag cctcgcggcg atcgtccagg aggaagcgtt
     1261 cgagtacctg gacgcgcccg tgcggatcgt cggcgcgctc gacacgcccg tcccctactc
     1321 ccctccgctc gaggaagcct ttctcccgag cgaggccgag gtcgagcgcg ctgcgcggtt
     1381 gcttctcgcc tactgaaagg aaacggttcg gggctcgggc gaccggactt tcgccaggaa
     1441 cgctccggac aggagcgcca cggcgaagca acccgaccac agcgctgcgg gcccgagccg
     1501 ttcgaggacg aagacgccga gcgccggccc ggcgacgaag gcgaggctga acgccatctg
     1561 gtagagcccc atgtactcgc cgcggcgcgc cggcggcgcg agctccactg ccagggcgtt
     1621 cagcgcgccg aaagtgaaga tctcgccgaa ggtccagacg acgatcgtcg ccgcgacggc
     1681 gacgggggtt cgcgcgaacg cgagcgcgcc gaagccgaag gcgatgagcg cgctcccgaa
     1741 gagaagagca cgccgatgcg gccagcgcgc agtcgccgcg ttgatcggaa ggtcgagaat
     1801 cacgatcagc acggtattga tcgcgagcaa gagaccatat ccggacggcg ggacgccgag
     1861 gtcccgaacc acgtagagcg acatggcgct cagacactga aacacgacgg cgaggatcgg
     1921 gaccatcgcg gcgacaaaga gtacgaagtc gcggtcccgc agggccgccc acggtgggcg
     1981 cacgtcatcc cccacacgga cggttcgctt cggcaggccc atccgcccgg cgagaacgat
     2041 caggatgatc ccggcgagca cggaggtcgt cccgtcgacc acgaagatcg cggggaacga
     2101 gcgttccgcg agcagccctc cgatgaccgg cccgattgag agcccgaggt tggccgcgag
     2161 ccgaacaagt gagaacgccg gcttcagctg ctctggcggc gtgatctcgc cgatgatcgc
     2221 gagactcggt gcgcggtacg cttcggagac ggaagcccac aaaaggactc ccgcgacgag
     2281 cggaacgaag gccctggccg tcggaaggag gaccgccagg aggcccccca cgacgagcga
     2341 cgtctgcatg atcgagagcg gagggaatcg gtccgcgagg cggccggcga acggcgccgc
     2401 caggagcgag ccgagtccga acgcagtgac cgccagtccc gctcgttcgg ccgtgagtcc
     2461 caggtcccgc gtcgcgtaga ggacgaggaa cgccaggatc atcgatcccg cgcgattgac
     2521 caggaacgcg cccgagatca cccagacgcc tttcggcagc cggcggaggc tcttccagac
     2581 gttcatggag tctcggagaa ttgtagaagc ggcccgacca tatgattcgg gcggtccgat
     2641 gatgttcgct cccggaagca agcatggtcc attcgagatc ctcgggccgc tcgggtccgg
     2701 agggatgggt gcggtgtacc gggcgcgcga ctcgcggctg caccgtgacg tcgccatcaa
     2761 ggcgctgcct ccggatttcg cctccgaccc ggagcgcgtc gcgaggctct cccgcgaggc
     2821 gcggctcctc gcttcgctga atcaccccaa cattgcggcg atctatgggc tcgaggaagc
     2881 cgacggttcg ccgttcctcg tcatggagtg cgtcgagggc gacagcctcg cccagaagct
     2941 ggctgcggga ccgctgccgg tggacgacgc gctcgcgatc gggacgcaga tcgcggcagg
     3001 gttggaggcc gcgcacggcg caggagtgat tcaccgcgat ctgaaacccg cgaacgtgat
     3061 gatccgcggg gacgggtcgg tcaaaatcct cgacctgggg ctcgcacggt ccgtcgagac
     3121 ttcgaccgcg ccgtccgatc cgtcgctctc gccgacgata ccctccggcg ggacggcgac
     3181 cggggtgatc ctcggcacgg ccgcttacat gagccccgag caggcgcgcg gccgggcgct
     3241 cgacaagcgc accgacgtct tttctttcgg ttgcgtgctc tacgaatgcc tgacgggccg
     3301 gcgcgcgttt ccgggcgaga caatctccga cactctcgcg gcgatccttc gcggagaacc
     3361 ggattgggaa gcgcttcctc ccgacacgcc cgaacaggtc cgccgcgtgc tccaccgctg
     3421 cctgcagaaa gacccgcgaa agcgcctcca tgacatcgcg gacgcccgga tcgacctcga
     3481 ggaagcgatc tcgtcgccgc acgcttttga aaccgcgacg ccggtcgtgg cgcgttcgag
     3541 acgcgggccg gcccttctct gggcgctcgc cggcgcattg atcgggatcg tggcggtcta
     3601 ctccgttggc cgtctgttcg gccgccccgc gtcggcacca tcgaagccgg tgcgcgccgt
     3661 cctgcctcta cctccgaata cgaggctcgg cttcgcgggt cgtccggcga tcgcggtgtc
     3721 tcccgacagt cggacagttg tcttcggggc ggtcgagggg ggaatccccc gcctttatcg
     3781 ccgcgacctc ggtggatcgg aagcgacggc gattgcgggc acggaagggg gatttgcacc
     3841 gttcatctcg cccgacgggg agtggctcgg cttcttcacg ccgatcgaga tgaaaaaggt
     3901 tcctctggcg ggcggctcgc cgatccggat ttccctcgtg ccgccggtga ccgccggagg
     3961 agcctgggat gccgacggaa ccatcgtctt tactctgacg gcgaacggcc cgctttcccg
     4021 cattcccgac gccggcggca agatccaggc ggtatcggcg ctcgacaatt cccgcggaga
     4081 gcactcccat ctctggcctc agatcctgcc ggaccggcgc gggatcctcg tgacgatggt
     4141 cgttgggagg gacttccagg acttcgacaa cgcgcagatc gtcgtcctcg acccgaagac
     4201 cggccaccgt accgtggtgc tcgaaggcag tcctttcgcg cgctacgcgg cggggcagat
     4261 cttcttcgtc cgaggcggct cgatgttccg ggcaccgttc gacctgtccc ggctctccgt
     4321 gacgggcccg cccgttcctc tcgcggaaag ggtcacgatc gagtccggca acggtacggc
     4381 gagcttcgcg atcacgaacg acggaacact cgtgtacgcg gacggcccgc aggttccccc
     4441 agtccagact gtcgtgctgc agctcgatcg acgggggatc gaaaagccgc tgcc
//
LOCUS       DASWAV010000112         2617 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U2665
            k141_645645, whole genome shotgun sequence.
ACCESSION   DASWAV010000112 DASWAV010000000
VERSION     DASWAV010000112.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960316
            Sequence Read Archive: SRR10260007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2617)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2617)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:47:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,929
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2617
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_645645"
                     /isolate="SMAG_U2665"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.36444 N 132.31889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2042)
                     /locus_tag="VF958_02365"
     CDS             complement(<1..2042)
                     /locus_tag="VF958_02365"
                     /inference="COORDINATES: protein motif:HMM:NF025006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEX9147984.1"
                     /translation="MKNRFTKLAALVLVALGIAAGAFAATTGTLFGTITDSDGSALPG
                     VTVTITSPGLQGSRTATTNPAGEYNFPLLPPGEYRVEYTLSSFETVVRTGVVVNLDQK
                     TKVNVTLALARVAESVEVSGESVVIDPTQTNTQVNLKEDHLKYAAIGLANRSYQNVIL
                     EAPGVSSQTGAGGNPSVFGSNLGQNSFLIDGLNTTDPVTHTFTSNFTYDSIQEIAVQT
                     SGFEAEYGKAIGGIINVITKSGGNDFHGSLDARYRSQDLTEQGTRKRDYPPGTDVLRY
                     DKDTQDFHQLSPAATFGGPLLRDRVWFFGSLERPDFTNQRPDLFGFQPGRRDFKGWTI
                     FGKVTATPAPSHTVALKFNSETADIDHAEDSSFYSPEAGYIQKQDLRVYNGSYDAVLT
                     PSWLANFQLGYMENKLDSRPVSGDLDTTGTIDQVTGIATGNYTNIQFSKRPRLQALGS
                     TSYIADFLGSHVFKVGTDLEWTKFSNVNNVTGTPIDPSFCSEQFGQPAGVSCGAINEP
                     ADGDPFLYLVYTDVPEQTFKGHGMAFYGQDEWRPIANLTAKLGVRYDEADFFLPGDEK
                     VKTFARFQPRVGVAWDIFNNSSTIVKANAGEFMEDNALTLPSFLSRQGSVLSLFLWSN
                     AQQRFRFFGAFGGPSGNGLDPSLRPTYSQQVSGGVTQRIFRNTSVDVTGIYRRNRN"
BASE COUNT          434 a          794 c          884 g          505 t
ORIGIN      
        1 atgttccggt tgcgccggta gattccagtg acgtcgacgc tcgtgttgcg gaaaatccgt
       61 tgcgtgactc ccccggacac ctgctgcgag taggtcggcc gcaacgaggg atcgagcccg
      121 ttcccggacg ggccgccgaa ggcgccgaag aaacggaaac gctgctgagc gttgctccag
      181 aggaacagcg acaggacgga gccctgccgc gagagaaaac tcgggagcgt gagggcgttg
      241 tcctccatga actcgccggc gttcgccttc acgatcgtcg aggagttgtt gaagatgtcc
      301 caggcgacgc cgacgcgcgg ctggaagcgc gcgaacgtct tcaccttctc gtcgccgggc
      361 agaaagaagt ccgcctcgtc gtagcgcacg ccgagcttgg ctgtgaggtt cgcgatcggc
      421 cgccactcgt cctggccgta aaacgccatg ccgtggccct tgaacgtctg ttcgggcacg
      481 tccgtgtaga cgagatagag gaacgggtcc ccgtcggcgg gctcgttgat cgcgccgcag
      541 ctgacgccgg ccggctggcc gaactgctcc gagcagaagc tcgggtcgat cggcgtgccg
      601 gtcacgttgt tgacgttgct gaacttcgtc cactcgaggt ccgttccgac cttgaagacg
      661 tgacttccga ggaagtcggc gatgtagctc gtcgatccga gggcctggag gcgggggcgc
      721 ttggagaact ggatgttcgt gtagttgccc gtcgcgatcc cggtcacctg gtcgatcgtg
      781 ccggtcgtat cgaggtcgcc gctcacgggt cgggagtcga gcttgttctc catgtagccg
      841 agttgaaagt tggcgagcca gctcggggtc aggacggcat cgtaggagcc gttgtagacc
      901 cggaggtcct gcttctggat gtatcccgcc tcggggctgt agaaggagct gtcctcggcg
      961 tggtcgatgt cagccgtctc gctgttgaac ttcagagcga cagtgtggct cggggccggg
     1021 gtcgcggtga ccttgccgaa gatcgtccag cccttaaagt cccgccggcc gggctggaag
     1081 ccgaagaggt ccggccgctg gttcgtgaaa tccggtcgct cgagcgaacc gaagaaccac
     1141 acccggtcgc gcagcagcgg accgccgaac gtcgcggccg gcgacagctg gtggaagtcc
     1201 tgggtgtcct tgtcgtaccg aagcacgtcg gtgcccggcg ggtagtcgcg cttgcgcgtt
     1261 ccctgctcgg tcaagtcctg tgagcggtac cgcgcgtcga gagatccgtg gaagtcgttg
     1321 cctcccgact tggtgatgac attgataatt ccgccgatcg ccttcccgta ctccgcctcg
     1381 aagccgctcg tctggacggc gatctcctgg atggagtcgt aggtgaagtt cgacgtgaag
     1441 gtgtgcgtga cggggtcggt ggtattcagc ccgtcgatca ggaacgagtt ctgtcccagg
     1501 ttcgagccga agaccgacgg gttaccaccc gctccggtct gcgaggaaac gccgggagcc
     1561 tcgagaatga cgttctgata ggaacggttc gcgaggccga tcgcggcgta cttcaagtga
     1621 tcctctttca ggttcacctg cgtgttggtc tgcgtggggt cgatgacgac gctttcgccg
     1681 ctcacctcga ccgactccgc gacgcgcgcg agcgccagcg tgacgttcac cttggttttc
     1741 tgatcgaggt tgacgaccac ccccgtgcgc acgacggttt cgaaggacga gagcgtgtac
     1801 tcgacgcggt actcacccgg gggaagaagc gggaagttgt actcccccgc cgggttggtc
     1861 gtcgcggtcc ggctcccctg gagccccgga ctcgtgatcg tcaccgtcac acctggaagc
     1921 gcgctcccgt cggagtcggt gatcgttccg aagagtgtcc cggtggtggc ggcgaaggcg
     1981 cccgccgcga tgccaagagc gaccagaacg agcgccgcaa gcttcgtaaa tcgattcttc
     2041 atggctcttc ctttcatcct gtttcctggt ctcgggtttc gtttcccgat tggcgcagga
     2101 gagcaacgcg gttgccacgg gccctcgagg gcgtcgcgcg acggagccac cgccaccgag
     2161 gacgaacgag tcgcgcgaat ggacggatcg ttcggcgcga aatcgagacg gggggccgct
     2221 ccagatctcc ggacggttcc tcaaatactt ggatggcggc gccaagcgtt ggtcccgatc
     2281 cgcgatctct cctgtgtcat gcgagccgga aaaagaggga cgaagaagcg tggtgtcggg
     2341 acagcggtgg attggctgcc gccgttcgcg aaggcgtccg atcggccgga tttcaagagc
     2401 tttgtgcgca gtctccggcg agcgtgccgt ccacgcgggg ccgcatggcg aaaaactatt
     2461 tcggagccgt cctccgaaag cgcgacttcg tcctctccgc gctgaccgac cgccgttcgc
     2521 ggcagccaac ttgctgtccc cgaagcccgt gggccgatcc ccgaaaactt tttcttcgtc
     2581 cgagccgttt acacaggcag ggagctctgc gacgttc
//
LOCUS       DASWAV010000113         3368 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U2665
            k141_166463, whole genome shotgun sequence.
ACCESSION   DASWAV010000113 DASWAV010000000
VERSION     DASWAV010000113.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960316
            Sequence Read Archive: SRR10260007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 3368)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3368)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:47:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,929
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3368
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_166463"
                     /isolate="SMAG_U2665"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.36444 N 132.31889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1535)
                     /locus_tag="VF958_02370"
     CDS             complement(<1..1535)
                     /locus_tag="VF958_02370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9147985.1"
                     /translation="MSGCFSALAEELFDSIELLQAGDPFRRVDVVVSSNYLAVDLRRR
                     LAERIAGAGRRAGHANVRFSTFLDIARDVAGEVSGKPAPPGILFAAVAGAVGSAPEVE
                     PFRGLRHRPGFLRSVEATVRDLRDAGVSAREFSTWAATSPMERRDALATLAEVYTDVS
                     RRLAPYADDVAVFRSAIGTAGENPSAEPLLVFGFYDLTGIQRDLVAALARARPLHLFL
                     PAFEGDLGLFGKGTREFLEELLGSPQSVPPAMATDAKSDWFARFAAMGTGPKPPLPAD
                     GSIRLVSAADDTDEAREVAREVLRSHADGIPLGRTAVILRQEESDAARILAELARAGI
                     PAFRLAGESNSEAPEGRAIRYWLQLEEDGFRREDVLDVLELVETARGGSSSALFRMLA
                     SQAGIVRGVEDWERGTGKLLRARPEDEEEESEKRSGSAARTASGHDAAERLAGAWTAL
                     RESATGWSDQPLSWTRWAEELRGRIARLFAPQEPPELIASAADSIASLDSPGQLVGKH
                     VAL"
     gene            complement(1675..1854)
                     /locus_tag="VF958_02375"
     CDS             complement(1675..1854)
                     /locus_tag="VF958_02375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9147986.1"
                     /translation="MNPNTPAPKTPETPTPDDAMTRKEKEDLQDQIEEFEGPDRREIR
                     GSNKEPADDPGVAHS"
     regulatory      1915..2047
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            2038..>3368
                     /gene="thiC"
                     /locus_tag="VF958_02380"
     CDS             2038..>3368
                     /gene="thiC"
                     /locus_tag="VF958_02380"
                     /EC_number="4.1.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013914257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomethylpyrimidine synthase ThiC"
                     /protein_id="HEX9147987.1"
                     /translation="MSSSREALPGNEVADRRPLPGSKKVYVPGPGDSRVPFREIALKP
                     TRGLKGEVELNPPLRVYDTSGPYTDPESEIDVRAGLAPLRRPWILGRGDYEETEPRRP
                     NAPDLSMTRPRKVLRCRANATQMHWARKGIVTPEMEFVGVRENVPAEFVRSEIARGRA
                     ILPANVNHPESEPMVIGRNFLVKINANIGNSAVTSSIEEEVEKMTWAIRWGADTVMDL
                     STGRNIHETREWILRNSPVPIGTVPIYQALEKVDGKAEELTWEIYRDTLIEQAEQGVD
                     YFTIHAGVLLRYIPLTAKRVTGIVSRGGSIMAKWCLAHHRESFLYTRFDEICEILKTY
                     DISFSLGDGLRPGSTADANDEAQFAELETLGELTTKAWAHDCQVMIEGPGHIPMHLVE
                     ENVRKEIEICHEAPFYTLGPLVTDIAPGYDHITSAIGAAMIGWFGTAMLCYV"
BASE COUNT          579 a         1158 c         1132 g          499 t
ORIGIN      
        1 tcgagcgcca catgtttccc gacgagctga ccgggggaat cgagcgacgc gatcgagtcg
       61 gctgccgacg cgatcagttc cggcggctcc tgcggggcga agaggcgcgc gatgcggccg
      121 cgcagctcct cggcccaacg agtccacgaa agaggctgat cgctccagcc ggtcgcggac
      181 tcccgcagcg ccgtccacgc cccggcgagc ctttcggcgg catcgtggcc cgacgccgtt
      241 cgcgccgcgc tccccgagcg cttctcgctc tcttcctcct catcctccgg ccgggcgcga
      301 aggagcttcc cggtacctcg ctcccagtcc tcgactcctc tcacgatgcc ggcctggctc
      361 gccagcatgc gaaaaagagc ggagctcgat ccccctcgcg ccgtctcgac gagctcgaga
      421 acatcgagca cgtcctcgcg ccggaagccg tcttcttcga gctgaagcca gtaccggatt
      481 gcgcgtccct ccggagcctc cgagttggac tcgcccgcga gccgaaaggc cgggatgccc
      541 gcccgggcga gctcggcgag aatccgcgcg gcgtccgatt cttcctgccg caggatcacc
      601 gcggtcctcc cgagggggat tccgtcggcg tgagaccgga gaacctcacg ggcaacctct
      661 cgcgcctcgt cggtgtcgtc ggccgcggag acgagccgga tcgacccgtc cgccgggaga
      721 gggggcttcg ggcccgtgcc catggccgcg aaccgcgcga accagtcgct cttcgcgtcg
      781 gtcgccatcg cgggcggaac gctctgcggg gagccgagaa gctcctccag gaactcccgc
      841 gtccctttcc cgaacagccc gaggtcccct tcgaacgcgg gcaggaagag gtggagcgga
      901 cgcgcccgcg cgagagcggc gacgaggtcc ctctggatcc cggtcaggtc gtagaacccg
      961 aagacgagga gcggctccgc tgacgggttc tcgcccgccg ttccgatggc cgaccgaaat
     1021 acggcaacgt cgtcggcgta tggcgccagc cgcctggaaa cgtccgtgta gacctcggcg
     1081 agagtcgcga gcgcgtcccg tcgttccatc ggggaggtgg ccgcccacgt cgagaactcg
     1141 cgcgcggaca cgcccgcatc ccggaggtcg cggacggtcg cctcgacgga ccgcaggaac
     1201 ccgggccgat ggcgcagtcc tcggaacggt tcgacctcgg gcgcggaacc cactgctccg
     1261 gcgacggctg caaacaggat ccccggaggg gccggcttac ccgacacctc acccgcgacg
     1321 tcccgggcga tgtcgagaaa cgtggaaaaa cgcacgttcg cgtggcccgc gcgacggccc
     1381 gctccggcga ttcgttcggc gagccgccgc cggagatcga cggcgaggta gttggaggag
     1441 acgacgacgt ccacgcgccg aaaggggtcc ccggcctgca gcagctcgat cgaatcgaag
     1501 agctcctccg ccagagcgga gaagcagccg ctcacgagac ggtgagccgc ccggtcgccg
     1561 ctagaggtcg acgggctcag ctcggcaaaa aggggcagat cgggagacac gcgagaaagg
     1621 ttatctcgct cgtccctcaa acactgtccg agccaggaag gtcggttcga tcgtctacga
     1681 gtgcgcgacc ccggggtcgt cggccggctc tttattgctg ccgcggatct cgcggcggtc
     1741 ggggccctcg aactcctcga tctggtcctg gagatcctcc ttctccttcc gggtcatcgc
     1801 gtcgtcggga gtcggagtct cgggtgtttt cggtgccggg gtgttcgggt tcatagagcc
     1861 tccggagact catcaattca agagccgcgc cagaaccgag gcgtagaatc gcggcggatc
     1921 gaccggggga gccgactccg ctgccgttcg ggttgcggaa ggctgagagt tctcgagtgc
     1981 cgtctcggga agaccccgtg aacctgacgc gggtaatgcc gccgtaggga ggaaccaatg
     2041 agcagttcac gcgaggcgct gccgggcaac gaagtcgcgg accgacgtcc gctgccgggc
     2101 tcgaagaaag tctatgttcc cggccccgga gactcacgcg tgccgttccg ggagatcgcg
     2161 ctcaagccga cgcgcgggct caaaggcgaa gtcgagctga accctccgct ccgggtctac
     2221 gacacgtcgg ggccgtacac cgacccggag tcggagatcg acgttcgcgc cggccttgcg
     2281 ccgctccgcc ggccctggat cctcgggcgc ggggattacg aggagaccga gccgcgccgc
     2341 ccgaacgcgc ccgacctctc gatgacacgc ccccggaagg tcctgcgctg ccgcgccaac
     2401 gccacccaga tgcactgggc gcgcaagggc atcgtcaccc ccgagatgga gttcgtcggg
     2461 gtccgcgaga atgtcccggc cgagttcgtc cggagcgaga tcgcgcgcgg ccgcgccatt
     2521 ctcccggcga acgtcaacca ccccgaatcc gagccgatgg tgatcggacg aaacttcctc
     2581 gtgaagatca acgcgaacat cggcaactcg gcggtgacct cctcgatcga ggaggaggtc
     2641 gagaagatga cgtgggccat ccgctggggc gcggacacgg tcatggacct ttcgaccggg
     2701 cggaacatcc acgagacccg cgagtggatc ctgcggaact ctccggtgcc gatcgggacc
     2761 gtgccgatct atcaagcgct cgagaaggtg gacgggaagg cggaagagct cacgtgggag
     2821 atctaccgcg acaccctgat cgagcaggcg gaacagggag tcgactactt cacgatccac
     2881 gcgggagtgc ttctccgcta cataccgctg accgcgaagc gcgtcacggg gatcgtgtcg
     2941 cgcggcggtt cgatcatggc gaagtggtgt ctcgcgcacc accgggagag cttcctgtac
     3001 acgaggttcg acgagatctg cgagatcctg aagacctacg acatctcgtt ctctctcggt
     3061 gacggcctgc gcccggggtc gaccgccgac gcgaacgacg aagcgcagtt cgccgagctc
     3121 gaaactctcg gcgagctgac cacgaaggcc tgggcgcacg actgccaggt catgatcgaa
     3181 ggccccggcc acatcccgat gcacctcgtc gaggagaacg tccgcaagga gatcgagatc
     3241 tgccacgagg cgccgttcta cacgctcggt cccctcgtga cggacatcgc tcccggctac
     3301 gaccacatca cgtccgcgat cggcgccgcg atgatcggct ggttcgggac cgcgatgctc
     3361 tgctacgt
//
LOCUS       DASWAV010000114         1897 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U2665
            k141_605892, whole genome shotgun sequence.
ACCESSION   DASWAV010000114 DASWAV010000000
VERSION     DASWAV010000114.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960316
            Sequence Read Archive: SRR10260007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 1897)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1897)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:47:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,929
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1897
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_605892"
                     /isolate="SMAG_U2665"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.36444 N 132.31889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..423
                     /locus_tag="VF958_02385"
     CDS             <1..423
                     /locus_tag="VF958_02385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011366067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9147988.1"
                     /translation="MSSNFPILLQLIQSDGRGDWHEVNEAGTKVQAVTAADVKRVANK
                     YFTRENRAVAIYTRKAGAAGPEDPDLAGLDDRGKQMVRQLTQRLATANDPERLRQMLS
                     QMDSQSAGAPPEMKPAMEVIRKKVQARIDELEKAKGKG"
     gene            420..>1897
                     /locus_tag="VF958_02390"
     CDS             420..>1897
                     /locus_tag="VF958_02390"
                     /inference="COORDINATES: protein motif:HMM:NF017044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HEX9147989.1"
                     /translation="MKRMRVASVALAAVLGASVASAQAASARPTRTSIPPRPEKLTFP
                     SLVYAPPTPADYRVKLASGPVAYVVPDRELPLVNVVVYVRAGQYLVPAGKEGLADLAG
                     YLLARGGTKSKTAEELEERLAFLAAQLNSAVGEAQGSVSLNLLSKDLDEGLSILREVL
                     SAPRFQDDKIALRKQQMMQAMKQRNDESAAIEARERGFLSFGEEFFANRYETAASVGS
                     LSKADLETFHRRWFHPAQFVVAASGDFDRATMIAKLETLFADWPYRGDAAPAVPTNTE
                     FAKPGVYIVDKDVNQGRVSVLLPGIRRDDPDFFPILVMNDVLGGGGFTSRITNRVRSD
                     EGLAYSAGSVFPGGAYYPLTFRAGFQSKSRTVAYATSVVVEEIKRIASEPVTAEELNT
                     AKKSFVETFPRTFATKAQVAITFAQDELTGRYASDPDYWKNYRAKIDRVTAADVQRVA
                     KKFLDSDRLVILIVGKKSDILAGDLKHKVELQTLAGNRLVDLP"
BASE COUNT          336 a          610 c          658 g          293 t
ORIGIN      
        1 atgtcgtcga actttccgat cctcctccag ctcattcaga gcgacggccg cggggactgg
       61 cacgaggtga acgaggcggg cacgaaggtg caggccgtga cggccgccga cgtgaagcgg
      121 gtcgcgaaca agtacttcac gagggaaaac cgcgccgtcg cgatttacac gcgcaaggcg
      181 ggcgcggccg gtccggagga tccggatctc gccggcctcg acgatcgggg caagcagatg
      241 gtgcggcagc tcacgcagcg tctcgcgacc gcgaacgacc ccgaacggct ccgccagatg
      301 ctctcgcaga tggattcgca gtcggcgggc gcgcctccgg aaatgaagcc ggcgatggag
      361 gtcatccgca agaaggtcca ggctcgcatc gacgagctcg agaaggcgaa ggggaaggga
      421 tgaagaggat gagagtcgct tccgtcgcgc tcgccgcggt cctcggcgcg tccgtggcgt
      481 ccgcgcaggc cgccagcgcc cgcccgaccc ggacctcgat cccgccgcgg ccggaaaagc
      541 tcacgttccc gtccctcgtc tacgcgccgc cgacgccggc ggactatcgc gtgaagctcg
      601 cgagcggacc cgtcgcctac gtcgttcccg acagggagct cccgctcgtc aacgtcgtcg
      661 tctacgtccg cgccgggcag tatctcgtgc cggccggcaa ggaaggtctg gcggacctgg
      721 ccgggtacct cctcgcgagg ggcgggacga agtcgaagac cgcggaggag ctcgaggagc
      781 gcctcgcgtt cctcgcggcg caactcaact cggcggtggg cgaggcccag ggcagcgttt
      841 cgctgaacct cctgtcgaag gatctcgacg aggggctgtc gatccttcgc gaggtccttt
      901 ccgcgccgcg atttcaggac gacaagatcg cgctgcgcaa gcagcagatg atgcaggcga
      961 tgaagcagcg aaacgacgag tccgccgcga tcgaggcgcg cgagcgtggg tttctgtcgt
     1021 tcggggagga gttcttcgcg aaccgttacg agacggccgc ttcggtcggg tcgctgtcga
     1081 aggcggacct cgagacgttc caccggcggt ggtttcaccc ggctcagttc gtcgtcgccg
     1141 cgagcggcga cttcgatcgc gcgacgatga tcgcgaagct cgagacgctc ttcgccgact
     1201 ggccgtacag gggggacgcc gcgccggccg tcccgacgaa cacggagttc gcaaagccgg
     1261 gcgtgtacat cgtcgacaag gacgtcaacc aggggcgcgt ctcggtgctc cttcccggga
     1321 tccgacgcga cgatccggac ttctttccga tcctcgtgat gaacgacgtc ctgggcgggg
     1381 ggggcttcac ctcgcgaatc acgaatcgcg tccgttctga cgaaggtctc gcgtacagcg
     1441 ccggttcggt gttcccgggc ggcgcgtact atccgctgac cttccgcgcg gggttccagt
     1501 cgaagtcgcg cacggtggct tacgcgacct cggtcgtcgt ggaggagatc aagaggatcg
     1561 cgtccgagcc ggtcacggcg gaagaactga acaccgcgaa gaagtcgttc gtcgagacgt
     1621 tcccgcggac gttcgcgacg aaggcgcagg tcgcgatcac gttcgcgcag gacgagctca
     1681 ccggccgcta cgcgagcgac cccgactact ggaagaacta ccgggcaaag atcgacaggg
     1741 tgacggcggc cgacgtccag cgtgttgcga agaaattcct cgactccgac aggttggtga
     1801 tcctgatcgt cgggaagaag tcggacatcc tcgccggcga tctcaagcac aaagtcgagc
     1861 tgcagacgct cgcgggcaac cggctcgtgg atctgcc
//
LOCUS       DASWAV010000115         4828 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U2665
            k141_565640, whole genome shotgun sequence.
ACCESSION   DASWAV010000115 DASWAV010000000
VERSION     DASWAV010000115.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960316
            Sequence Read Archive: SRR10260007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 4828)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4828)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:47:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,929
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4828
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_565640"
                     /isolate="SMAG_U2665"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.36444 N 132.31889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1794)
                     /locus_tag="VF958_02395"
     CDS             complement(<1..1794)
                     /locus_tag="VF958_02395"
                     /inference="COORDINATES: protein motif:HMM:NF017745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF885 domain-containing protein"
                     /protein_id="HEX9147990.1"
                     /translation="MIVPFLLLGAAPARRPAKAPSAAAKSAAVSKELAKFFDDEWELG
                     LRENPERGTYLGDPRYNDRLTDLSFEAIERRNKHEQEALARLKKIDRALLSEDDRLSY
                     DLYQRRLEIAIEGQRFPTELMPINQLEGLHKDFAILSTVTSFKTVKDYRDYLARLSAF
                     PKQVDQTIALMERGRQKGWVYPGVPLRDVPDAIRAQIHPDPEKSVHWKPFEKFPEGVP
                     EPDREALRIEGRRRIANDVFPAYLKLYTYFTRDYLPAAKKEVGAWALPDGESYYAYAI
                     KHHTTTNKSAKEIHDTGLAEVARIRSQMEDIIKKVRFKGNFDAFVKFLRTDPRFYYKT
                     ADDLLEGYRDICKRIDPELIKLFGTLPRTPYGVIPTPDFEAPTSTTAYYRQGSPEAPR
                     PGYFVANTYRLETRPKYEMEALAIHEAVPGHHLQIALAQELTGLPQFRRHGFFTAFVE
                     GWGLYAESLGDEMGFYTDPYSRFGQLTYEMWRAVRLVVDTGMHAFKWDRQRAIDFMKA
                     NTAKTEHDITVEIDRYIVWPGQALAYKIGELEFKELRARAKKQLGERFDVRKFHDAVL
                     LSGALPLDVLDERIDAWIAREKAAPVKPVAPG"
     gene            complement(1868..3217)
                     /locus_tag="VF958_02400"
     CDS             complement(1868..3217)
                     /locus_tag="VF958_02400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014159834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dicarboxylate/amino acid:cation symporter"
                     /protein_id="HEX9147991.1"
                     /translation="MPDRSEQRPRAGRRPLPRHTKIFFGLILGLVLGLLVHEMAGDAP
                     WVLWTVANVAYPLGQIFLRLIFMIVVPLVFSSLVLGVLELGDVKKLGRIGLKTLGYTL
                     FASGISVAIGITMVNFFRPGAGVDPSVRAELSSTFATQSQKAIENARQAQSWADTLLS
                     LIPRNPLASAVDALSGEMLALMVFAVFFGVALARIRDERAEPLVDFFSAIQRVSMKIV
                     DWAMALAPIGVAALMFSLTARAGFSVVPRLGGYVGVVVGGLAIQMFVVYSAILKGLAG
                     VPPVEWFFRCREVILTAFSTASSNATLPTSLRVSEEKLGIPQRIGNFVLTVGATGNQN
                     GTALFEGVTVLFLAQVFGIHLTLSQQVTVVIMSILAGVGTAGVPGGSLPLIVIVLQTV
                     GIPPEGIGIILGVDRFLDMCRTTLNVVGDLVCATFVARSERELRFPAATAAPIVADA"
     gene            3411..4292
                     /locus_tag="VF958_02405"
     CDS             3411..4292
                     /locus_tag="VF958_02405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004385027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-type lectin-domain containing protein"
                     /protein_id="HEX9147992.1"
                     /translation="MKSKVFGLAFVLVAAAGTATAQQTINFANFCDTSAFTLNGTTAS
                     LNPNAQCVLRLINGTGQSGSAFVTSTFSLASDASFSTFFTFQIPNPVGASDVDGVGAD
                     GIVFAVQAVGNTAGGGGGGIGFQGINNSVGVELDTWNNGAGDANSGNHIGIDVNGNVN
                     SVALTPIPGRFNDGNVWYAWVDYNGVTDLLEARVSSTAARPAAASVSTTVNLPAILGT
                     TNAFVGFTSGSGAAGGTHEIVSWNLIGTFSPIGGTPTPTPIPGPSTPVPTLSFPMMAL
                     LALALGAVSIVFLVRRA"
     gene            complement(4458..>4828)
                     /locus_tag="VF958_02410"
     CDS             complement(4458..>4828)
                     /locus_tag="VF958_02410"
                     /inference="COORDINATES: protein motif:HMM:NF012894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HEX9147993.1"
                     /translation="PPAPMPPPAPPVVAPPPPPAPTTDTIDFDRGSARITNIAKARLD
                     EVALRLRENPRATVMITGYPDDGTASARRESLARQRAENAKAYLVDRHGIDASRITTQ
                     TDLTDTSHRGQALIVVTFNP"
BASE COUNT          835 a         1563 c         1613 g          817 t
ORIGIN      
        1 acccggggcc acgggtttga ccggcgccgc tttctcccgc gcgatccagg cgtcgatccg
       61 ctcgtccagg acatcgagcg gaagcgctcc ggagagcagc acggcgtcat gaaacttccg
      121 cacgtcgaac cgctccccga gctgcttctt cgcccgcgcg cgcagctcct tgaactcgag
      181 ctccccgatc ttgtaagcga gcgcctggcc gggccagacg atgtaccggt cgatctcgac
      241 ggtgatgtcg tgctcggtct tcgcggtgtt cgctttcatg aagtcgatcg cgcgctgccg
      301 atcccacttg aacgcgtgca tccccgtgtc gacgacgagt cggaccgcac gccacatttc
      361 gtaggtcaac tgcccgaacc tggaatacgg gtcggtgtag aagcccattt cgtcgccgag
      421 gctctcggcg tagagtcccc acccctcgac gaacgcggtg aagaatccgt ggcggcggaa
      481 ctgcggaaga ccggtcagct cctgcgccag cgcgatctgc aggtggtgcc ccggaacggc
      541 ctcgtggatc gcgagagcct ccatctcgta cttgggccgc gtctcgagac gatacgtatt
      601 cgcgacgaag tatcccgggc gcggagcctc gggcgagccc tggcggtagt acgcggtcgt
      661 cgacgtcgga gcctcgaaat cgggcgtcgg gatcacgccg tagggggtcc gcggcagggt
      721 tccgaagagc ttgatgagct cgggatcgat ccgcttgcag atgtcgcggt atccctcgag
      781 caggtcgtcg gcggtcttgt aatagaagcg cggatcggtt cgaaggaact tgacgaacgc
      841 atcgaagttc cccttgaacc ggactttctt gatgatgtct tccatctggc tccggatccg
      901 cgcaacttcc gcgaggcccg tgtcgtggat ctccttcgcc gacttgttcg tcgtcgtgtg
      961 gtgcttgatc gcgtacgcgt agtacgactc gccgtccggc aaggcccacg cgccgacctc
     1021 cttcttcgcc gccgggaggt aatctcgcgt gaagtacgtg tagagcttca ggtaggcggg
     1081 aaaaacgtcg ttcgcgatcc gacgtcgccc ttcgatgcgc agcgcctcac gatccggttc
     1141 cggcacgccc tccggaaatt tctcgaacgg cttccagtgg acgctcttct cgggatccgg
     1201 gtggatctgc gcccggatcg cgtcgggcac gtcgcggagt ggcacccccg ggtagaccca
     1261 gcccttctgc ctcccgcgct ccatgagcgc gatcgtctga tccacctgtt tcggaaatgc
     1321 cgagagccgc gcgaggtagt cgcggtagtc cttcacggtc ttgaacgaag tgaccgtcga
     1381 gaggatcgcg aagtccttgt gcagcccttc cagctggttg atcggcatga gctcggtcgg
     1441 gaaccgctgc ccttcgatcg caatctcgag acgcctctgg tagaggtcgt aagacagccg
     1501 gtcgtcttcc gacagcaatg cccggtcgat cttcttcagc cgcgcgagcg cctcctgctc
     1561 gtgtttgttg cgtcgttcga tcgcctcgaa cgacaggtcc gtcagccggt cgttgtaacg
     1621 cgggtcgccg agatacgtcc cgcgctcggg gttctcgcgc agtccgagct cccactcgtc
     1681 gtcgaagaac ttcgcgagct ccttcgaaac ggctgcgctc ttcgcggcag cggaaggcgc
     1741 cttcgccgga cggcgcgccg gcgcggcgcc gaggaggagg aaaggaacga tcatcagaag
     1801 agccgcccga gaaagcattg tgaactcctg ttcctgtctc gagtctgtgc ctcgcgtcac
     1861 ggcagcctta cgcgtcggcg acgatcggcg ccgcggtcgc cgccgggaag cggagctccc
     1921 gctccgaccg ggcgacgaac gtggcacaga cgagatcgcc gacgacgttc agggtcgtgc
     1981 ggcacatgtc gaggaaacgg tcgacgccga gaatgatccc gatgccctcg ggcgggatgc
     2041 cgacggtctg gagcacgatc acgatgagcg gcagcgatcc cccgggcact cccgcggtgc
     2101 ccacgccggc gagaatcgac atgatgacga cggtgacctg ctgcgaaagc gtgaggtgaa
     2161 ttccgaagac ctgcgcgagg aagagaaccg tgaccccttc gaagagcgcg gttccgttct
     2221 ggttgccggt cgcgccgacg gtgagaacga aattgccgat tcgctgcggg atcccgagct
     2281 tctcttcgga gacccggagg ctggtgggga gagtcgcgtt cgaggacgcg gtcgagaacg
     2341 ccgtcaggat gacctcccgg cagcggaaga accactcgac cggaggaacg ccggcaagcc
     2401 ctttcaggat cgcggagtag acgacgaaca tctggatcgc gagaccgccg acgaccaccc
     2461 ccacgtatcc gccgaggcgc ggcaccaccg agaacccggc gcgcgccgtg agagagaaca
     2521 tcagggcagc cacgccgatg ggcgcgagcg ccatcgccca gtcgacgatc ttcatggaga
     2581 cgcgctggat cgcggagaag aagtccacga gcggctcggc cctctcgtcg cggatccggg
     2641 cgagcgcgac cccgaagaac accgcgaaga ccatgagcgc gagcatctct ccggagaggg
     2701 cgtccaccgc ggaggcgagc gggtttctgg ggatgagtga gagcagggtg tcggcccagc
     2761 tctgcgcctg ccgcgcgttc tcgatcgcct tctgcgactg ggtggcaaac gtgctcgaca
     2821 gctcggcccg gaccgacggg tccacgcccg cgcccggacg gaagaagttc accatcgtga
     2881 tgccgatcgc aaccgagatc ccgctcgcaa aaagggtgta gccgagggtc ttcagcccga
     2941 tgcggccaag cttcttgacg tcgcccaact cgaggacgcc gagcacgagc gaggagaaca
     3001 cgagcgggac gacgatcatg aagatcagcc gaagaaaaat ctggccgagc gggtacgcca
     3061 cgttggccac ggtccagagc acccagggcg cgtcgcccgc catctcgtgc acgaggaggc
     3121 cgaggacgag tccgaggatg agtccgaaga agatcttcgt gtgtcggggg agaggacgcc
     3181 gcccggcgcg cgggcgctgc tcggaacggt cgggcatcag gcgggaactc taacgaatcg
     3241 gcacgggaga tttccggaaa atccgccggc cgaccgcggt cgtctcgacg ctctcgttcc
     3301 ccctgcgggc actcctggcg accgcgcgcg cggcagccgc gaaaaccccg ctccgggggg
     3361 tttccgcagc gcgtgctaga gttttacagc ttctttttgc gggaggaaaa atgaaatcga
     3421 aggtcttcgg cctcgcgttc gtgctcgtgg cagccgccgg cacggcgacc gcccagcaga
     3481 cgatcaactt cgccaacttc tgcgacacct cggcgttcac cctgaacgga accacggcga
     3541 gcctcaatcc gaatgctcag tgcgtgcttc ggctgatcaa cggcacgggc cagagcggca
     3601 gcgctttcgt gacgagcacc ttttcgctcg cgtcggacgc ctcgttctca accttcttca
     3661 cttttcagat tccgaacccc gtgggcgcga gcgatgtcga cggcgtcggc gcggacggga
     3721 tcgtcttcgc cgtgcaggcg gtgggaaaca cagccggcgg cggcggcggc ggaatcggat
     3781 tccagggaat caacaacagc gtcggtgtcg agctcgatac gtggaacaat ggagccggcg
     3841 acgcgaacag cggcaaccac atcgggatcg acgtcaacgg gaacgtgaat tcggtcgccc
     3901 tgacgccgat cccgggtcgc ttcaacgacg ggaacgtctg gtacgcctgg gtggactaca
     3961 acggcgtgac ggaccttctc gaggcaaggg tatccagcac agcggcccgg cccgcagccg
     4021 cgagcgtctc tactacggtg aaccttccgg cgatcctcgg gaccaccaac gcgttcgtcg
     4081 gattcacgtc cggctcaggc gcggccggag gcacccacga gatcgtctcg tggaacctca
     4141 tcgggacgtt ctctccgatc ggtgggacac cgacgccgac tccgataccg gggccctcca
     4201 cacctgtgcc gacgctgtct tttccgatga tggcgctgct cgcgctcgcg ctgggggccg
     4261 tttccatcgt cttcctggtg agaagagcgt aagcggctcc gtccgccttc ccgagaagac
     4321 cccgccgagg cggggtcttc cttttcggaa gaaagtcccg tcgccctgcc gccggggatt
     4381 cgggttcggg ccgttccccg ctccaacgaa aaggcccctc cggaaggagg ggccagaaga
     4441 gtgccgggac gcccgcgtca agggttgaac gtcaccacga tgagcgcctg tccacggtgc
     4501 gaggtgtcgg tcaggtccgt ctgggtcgtg atccgcgaag cgtcgatccc gtggcgatcc
     4561 acgagatatg ccttggcgtt ctcggcccgc tgccgcgcga gtgattcgcg gcgggcggac
     4621 gcggtcccgt cgtcgggata gccggtgatc atcaccgtcg cgcgcgggtt ctcgcgcagc
     4681 ctcagcgcca cctcgtcgag gcgggccttg gcgatgttcg taatccgggc gctgccgcgg
     4741 tcgaagtcga tcgtgtccgt cgtcggagcc ggagggggcg gaggcgccac gaccggaggc
     4801 gccggcggcg gcatgggcgc gggcgggg
//
LOCUS       DASWAV010000116         4354 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U2665
            k141_246987, whole genome shotgun sequence.
ACCESSION   DASWAV010000116 DASWAV010000000
VERSION     DASWAV010000116.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960316
            Sequence Read Archive: SRR10260007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 4354)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4354)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:47:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,929
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4354
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_246987"
                     /isolate="SMAG_U2665"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.36444 N 132.31889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            135..1706
                     /locus_tag="VF958_02415"
     CDS             135..1706
                     /locus_tag="VF958_02415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9147994.1"
                     /translation="MNRFPVMKVVLCALGGVLASGLVLAQVEPPLLPNGSGVPNWTVP
                     TYHPSSAEGGLRTMADISPGVGFVAMQPCRVFDTRNANGPYGGPRLLANTTRNFDIDS
                     GPCTGIPAGVEAYSMNFGAILPDGANSFITIWPAGTTQPGVSSINPVQGFVVANAAIV
                     PAGTGGAISVFPNTGVHLYGDINGYFTADYANNNYFQVHVNSTGYAIDASNSSTSCSG
                     TCGMRMSVDSGNGIEGQTHATSGATYGVFGNLFNATNGAAGIYGEGATGSCGTPSFSA
                     GVLGRAGAAASGEIPIIGMGDYEGVRGINTSGCTNTVATYGSLGYTDATAVYGSGNLV
                     ITGTKSFADPHPTDPSKMIAYVSLEGPESGTYFRGKARFQNGVAVIEVPEHFRMVTDP
                     EGLSIQVTPIGQMATVAVESIGLDRIVVRGSRNVEFFYTVNGVRTTFKDFQPIQDNIA
                     FVGTAEDAGMPGAWSPEQKRRLVASGAFNPDGTVNRETAHRLGWDSFWESRRPQRESK
                     PSTASPSKAPVSSNQ"
     gene            1821..3350
                     /locus_tag="VF958_02420"
     CDS             1821..3350
                     /locus_tag="VF958_02420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9147995.1"
                     /translation="MTSSRLTPVLLVLLSVSSPVCAQIGAPVSNWTVPPSVHRTDSVA
                     GLAPMADVTQLSIFVAVAPCRVVDTRGPAGPYGGPALTANVTRSFNIESGPCSGIAPL
                     PGAFSLNFGAILPPADGFLTAWPVGTTQPTVSQLNLLGGEVVANAAIVPAGTSGSINV
                     RVNVGPTNIYIDINGYFVSEGTLNPNESLNLYGDTVGCLVCVHNANGSAFRAIDASMT
                     SGQNAGAVIWARNDASTGSHFGVTGIVDTVSDNSAGIFGLEGTSFVPGTHVTAGVRGE
                     SAIGGTGVLGLVSDPQGEGTTGFMLNGSNGLILSGGHLGYSSTVGVYFDNGLAGSGTK
                     SFVEPHPTDASKAIKFISLEGNEPGTYFRGRGRFQNGIAAIIVPEEFRMVTDREDLGI
                     QVTPIGEMATVAVESIDLDRIVVRGSRDVEFFYLVNGVRRAYKAEPIVVDNEKFFVPE
                     SPDARMPSYLRADEKRRLIENGTYRPDGTVNMETAKRLGWTRAWEAKKTVAPSPAPES
                     P"
     gene            3464..>4354
                     /locus_tag="VF958_02425"
     CDS             3464..>4354
                     /locus_tag="VF958_02425"
                     /inference="COORDINATES: protein motif:HMM:NF019607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HEX9147996.1"
                     /translation="MEAPNARRRRGTSAAARCSSLAVTLVLLAAAAHAATYSVAGFSD
                     TAVVSGLDQPTDFAFLPDGRILILEKPGRVKIAPSGGGVPVLALDITTSVDDAFEKGL
                     LGVCLHPQFATNGYVFLYYTTNTPRNRISRFTLTGSTLGSELVILDNIEATNGNHNGG
                     TVLIGNDGKLWAAPGDSGTGGTKAQILDAASPSRFNGKVLRMELDGSPPADNPFVGDP
                     TKEPRIWAYGFRNPFRFSFRPTNGSIYIGDVGQSTREELDVGVAGGNFGWPYMEGFIQ
                     QPGGPGPCPATTTCLPPVFDY"
BASE COUNT          778 a         1448 c         1353 g          775 t
ORIGIN      
        1 ttcacggacg acctgtctct tcggaagacg tttccgatca cggcggaccg accttgcgga
       61 ccggaaggag gagaactatc gagaaagaga ctcgttctct ctggatttcg tttttccaaa
      121 ttaggaggaa tcgcatgaac cggtttccag tcatgaaagt ggttctctgc gcccttggcg
      181 gcgtcctcgc ctccggcctg gtcctcgcgc aagtcgagcc gcctcttctc cccaacggct
      241 cgggcgttcc gaactggact gtccccacgt atcacccatc gagcgctgaa ggcggcctcc
      301 ggacgatggc cgatatttct ccgggcgtcg gcttcgtcgc catgcagccc tgccgggtct
      361 tcgacacgcg caacgcgaac ggcccgtacg gcggcccgcg gctgctcgcg aacacgaccc
      421 ggaacttcga catcgacagc ggcccctgca cggggatccc ggccggcgtc gaggcatact
      481 cgatgaactt cggagccatt cttccggacg gcgccaacag tttcatcacg atctggccgg
      541 ccggaacgac acaacccggt gtctcctcga tcaatcccgt gcagggcttt gtcgttgcca
      601 acgccgcaat cgtgccggcg gggaccggcg gagcgatcag cgtcttcccc aacacgggag
      661 ttcatctcta cggggatatc aacggctact tcacggccga ttacgccaac aacaactatt
      721 tccaggtcca cgtgaactcg accggttacg ccatcgacgc gagcaactcc tcgacctcct
      781 gctccggcac ctgcgggatg cgcatgtcgg tggacagcgg gaacggcatc gaaggtcaga
      841 cccacgccac gagcggagcc acctacggtg tcttcggcaa cctgttcaac gccacgaacg
      901 gtgccgccgg gatctacggc gagggtgcca ctggctcttg cggaacgccg agtttcagtg
      961 cgggcgtgct ggggcgagcg ggcgccgccg cctccgggga gattcccatc atcggaatgg
     1021 gcgactacga aggggtccgc gggatcaaca cgagcgggtg caccaacacc gtcgcaacct
     1081 acggatccct cggctatacc gacgcgactg cagtctatgg gagcggcaat ctcgtcatca
     1141 cagggacgaa atcattcgcg gatccgcatc ccacggatcc ctcgaaaatg atcgcgtacg
     1201 tctctctcga aggtccggag tcggggactt acttccgcgg gaaggcgcgg tttcagaacg
     1261 gcgtcgccgt tatcgaagtg cccgagcact ttcgaatggt gaccgatccg gagggcttga
     1321 gcatccaggt gaccccgatc ggccagatgg cgaccgtcgc cgtggagtcg atcggtctcg
     1381 atcgaatcgt cgtccgtggc tctcgaaacg tagaattctt ctacaccgtc aacggagtcc
     1441 ggacgacgtt caaggacttt cagcccattc aggacaacat tgcattcgtc gggactgcgg
     1501 aggatgccgg catgcccggc gcgtggagcc cggaacagaa acgtcgcctc gttgccagcg
     1561 gagcattcaa cccggacgga accgtgaacc gcgagactgc gcatcgtctc gggtgggact
     1621 ctttctggga atccagacgg cctcagcggg agagcaagcc gtcgaccgcg agcccgtcga
     1681 aggcgccagt cagcagcaac cagtagggtt ttctcccgtc atcgaagggc cgcggcgtgg
     1741 gccgcggccc tttctttgcc cgcgcccgtg cgacggcgtg atggaatccc tccaacggca
     1801 actctttccg gaggaaagcc atgacctctt ctcgcctgac tcccgtactc ctggttctcc
     1861 tgtccgtctc ttcgcccgtg tgcgcccaga tcggcgcgcc cgtctccaac tggacggtgc
     1921 caccctcggt gcaccggacc gactccgttg cgggcctcgc tccgatggcc gacgtcacgc
     1981 agctgagcat cttcgttgcc gtcgcgccct gccgcgtcgt cgacacccgg ggtcccgccg
     2041 gtccttatgg gggtccggcg ctcacggcca acgtgacgcg ttcgttcaac atcgaatccg
     2101 gaccttgttc cgggatcgct cccctgccgg gagccttctc gctcaacttc ggcgcgatcc
     2161 tccccccggc cgacggattt ctgacggcgt ggccggtcgg cacgacccaa ccgaccgtgt
     2221 ctcaattgaa cctcctgggc ggcgaggtcg tcgccaacgc cgcgatcgtg ccggcgggga
     2281 cgagcggctc gatcaacgtc cgggtcaatg tcggcccgac gaacatctac atcgacatca
     2341 acgggtactt cgtctccgaa ggaaccctca acccgaacga gagtctcaac ctctacggcg
     2401 acacggtggg ctgcctcgtg tgcgttcaca acgcgaacgg cagcgctttc cgagcgatcg
     2461 acgccagcat gacgtccggc cagaacgccg gggcggtcat ctgggcgcga aacgacgcga
     2521 gcaccgggtc tcacttcggc gtcaccggta tcgtggacac ggtcagcgac aactccgccg
     2581 gtatcttcgg actcgagggc acttcgttcg tccctggaac tcacgtgacg gcgggcgttc
     2641 gcggagagtc cgccatcggg ggaaccggcg tcctcgggct cgtgagcgat ccgcagggcg
     2701 aaggcacgac cgggttcatg ctgaacggct cgaacgggtt gattctttcg ggaggccatc
     2761 tcggctacag ctccacggtc ggcgtctact tcgacaacgg gctcgcgggg tccggcacga
     2821 agagtttcgt cgagccccat ccgactgacg cctcgaaagc gatcaagttc atatcgctcg
     2881 aaggcaacga gccggggacg tacttccgcg gccggggacg ctttcagaac gggatcgcgg
     2941 cgatcatcgt gccggaggag ttccgcatgg tcacggatcg ggaggacctc ggcattcagg
     3001 tgacgccgat cggagagatg gcgacggtcg cggtcgagtc gatcgatctg gatcgaatcg
     3061 tcgtccgggg atcgcgggat gtggagttct tctacctcgt gaatggagtt cggcgcgcgt
     3121 acaaggcaga gcccatcgtg gtcgacaacg aaaagttctt cgtgccggaa tcgccggacg
     3181 cgcggatgcc ttcctatctg agagccgacg agaaacgccg gctgatcgag aacggaacgt
     3241 accggccgga cgggaccgtc aacatggaga ctgcaaagcg tctcgggtgg acgcgcgcgt
     3301 gggaggcgaa aaagactgtg gcgccgagtc cggctcctga atctccctga gaacgcctcc
     3361 gaacctgcgt tgaacgggcc gcggcaccgc gtcgcggcct tctctttctc ttcttgaaag
     3421 accgtgtcga gtccgctatc ttggcggaga agcgacactt tcgatggaag cccccaacgc
     3481 ccggaggcgt cgagggacga gcgctgcggc gcggtgttcc tctctcgccg tcaccctcgt
     3541 cctcctcgcc gcggcggcac acgccgcgac gtattccgtc gccgggtttt cggacacggc
     3601 ggtcgtcagc ggcctcgatc aaccgaccga ctttgccttt ctgcccgacg gccggatcct
     3661 gatcctcgag aagccaggtc gggtgaagat cgctccctcg ggcggcggcg tcccggttct
     3721 tgctctcgac atcaccacga gcgtggacga cgctttcgaa aaggggcttc tcggggtctg
     3781 cttgcatccc cagttcgcga caaatggata tgtgttcctc tactacacga ccaacacccc
     3841 ccggaatcgg atctcccggt tcaccctgac cggcagcacg ctcggctccg agctcgtcat
     3901 cctcgacaac atcgaagcca ccaacggcaa ccacaatggc ggcacggtcc tgatcggcaa
     3961 cgacgggaag ctctgggcgg cgccaggcga ctcggggacg ggtggcacga aggcccagat
     4021 cctcgacgcg gcaagcccga gccgtttcaa cgggaaggtc ctgcggatgg agctcgacgg
     4081 gtctccgccc gccgacaacc cgttcgtggg cgacccgacg aaggagcctc gtatctgggc
     4141 ctacggcttc cgcaacccgt tccgattttc cttccggccg accaacgggt ccatctacat
     4201 cggcgacgtc ggtcagagca cccgcgagga gctcgacgtc ggggtcgccg gaggaaactt
     4261 cggctggccg tacatggagg gattcatcca gcagccgggc gggccgggcc cgtgcccggc
     4321 cacgacgacg tgtctccccc ccgtcttcga ctac
//
LOCUS       DASWAV010000117         1909 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U2665
            k141_127279, whole genome shotgun sequence.
ACCESSION   DASWAV010000117 DASWAV010000000
VERSION     DASWAV010000117.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960316
            Sequence Read Archive: SRR10260007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 1909)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1909)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:47:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,929
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1909
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_127279"
                     /isolate="SMAG_U2665"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.36444 N 132.31889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            186..>1909
                     /locus_tag="VF958_02430"
     CDS             186..>1909
                     /locus_tag="VF958_02430"
                     /EC_number="2.7.9.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012528116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate, phosphate dikinase"
                     /protein_id="HEX9147997.1"
                     /translation="MSVDKPVSDGSARVTRPGKRVYYFGDGKAEGAGLGKEALGGKGS
                     GLAEMTALGIPVPPGFTIDTSVCLDFLRTGDLEGLKPEVEEALGRVESAAGRKFGDPA
                     NPLLVSVRSGARSSMPGMMDTILNLGLTSRSVAGLAAAAGERFALDCRRRFLQMYSDI
                     VLDVPRSAFEEILTRKKQERRVASDADLTAQDLRWLVERFEDLVRQRTGAPLPDDPRE
                     QLWGAIGAVFRSWDNKRAKEYRRLHRIPEDWGTAVNVQTMVFGNRGDRSATGVAFTRN
                     PATGENQFYGEFLPNAQGEDVVAGIRTPQPLRKDGSGKSLEETMPEAYAGLVRVRDLL
                     EKNFRDMQDLEFTIEDGKLYLLQTRNGKRTGFAAVRVATDMVAEGLITETEAVTRVEP
                     EQLVQLLAPIFPSKEKEAAVKGGRLLAKGLPAGPGAACGRVAFTADRAVEMAAKGDPV
                     VLVRVETSPEDIAGMHAAVGILTTRGGMTSHASVVARGMGKTCVVGAGEITVNETKGE
                     LRARNLSAKEGDFLSVDGTAGEVILGKLPTQPSEILQVLIEGSLAPEKAPLFGAFTRL
                     LEWADRAR"
BASE COUNT          325 a          617 c          674 g          293 t
ORIGIN      
        1 accggttctt cgtcgaagtc ctggtcaacg tgcccgagga ggacgtgcgc cggaaccggc
       61 acgccctgct cggcgccatc cggcgggagt tcacgaagat cgcggatttt tctgagatag
      121 tcgtggagaa atgacatggt gcgcagccgt aatgcatctc aatctctttt cgcggccgga
      181 cggccatgag cgttgacaag ccggtgtccg acggcagtgc tcgcgtgacc cgaccgggga
      241 agcgcgtcta ctactttggt gacggtaagg ccgaaggcgc gggcctcggc aaggaagcgc
      301 tcggcgggaa agggtccggc ctcgcggaga tgaccgctct cggcatcccg gtgccgcccg
      361 gttttacgat cgacacatcg gtgtgcctcg attttctgcg gaccggagac ctcgagggcc
      421 tgaagccgga ggtcgaggag gcgctcggcc gggtcgagag cgcggccggc cggaagttcg
      481 gcgaccccgc gaatccgctt ctcgtgtcgg tccgctcggg ggcacgttcc tcgatgcccg
      541 gcatgatgga cacgatcctg aacctcggcc tgacgtcccg ctccgtcgcc ggcctcgcag
      601 cagccgccgg cgagcggttc gcgctcgact gccgccgccg cttcctgcag atgtactccg
      661 acatcgtcct cgacgttccc cgctccgctt tcgaagagat cctgacccgc aagaagcagg
      721 agagacgggt cgcctcggac gccgacctga ctgcgcagga cctccggtgg ctggtcgagc
      781 ggttcgagga cctggtccgg cagcgcaccg gggctccgct ccccgacgat ccgcgcgagc
      841 agctctgggg cgcgatcggc gccgtcttcc gctcctggga caacaagcga gcgaaggagt
      901 accgccggct ccatcgcatc cccgaggact gggggaccgc cgtcaacgtg cagacgatgg
      961 tgttcggcaa ccggggggac cgctccgcga ccggtgtcgc gttcacccgg aacccggcga
     1021 cgggcgaaaa ccagttttac ggggagtttc tccccaacgc gcagggggag gacgtcgtcg
     1081 cgggaatccg cacgccccag cctctgcgca aggacggctc cgggaagtct ctcgaggaga
     1141 cgatgcccga ggcgtatgcc gggctcgttc gcgtgcgcga cctcctcgaa aagaacttcc
     1201 gagacatgca ggatctcgag ttcacgatcg aggacgggaa gctctacctc ctgcagacga
     1261 ggaacggaaa gcgcacgggg ttcgccgcgg tgcgcgtcgc caccgacatg gtcgcggaag
     1321 gcttgatcac cgagaccgag gccgtgaccc gcgtcgagcc cgagcagctc gtgcagctgc
     1381 tcgcccccat atttccttcg aaagagaagg aagcggcggt caaggggggg cgccttctcg
     1441 cgaagggact gcccgccgga ccgggagcag cctgcggacg ggtcgcgttc accgcggacc
     1501 gcgcggtcga gatggcggcg aaaggcgatc cggtcgtgct cgtccgggtc gaaacgtctc
     1561 ccgaggacat cgcgggcatg catgccgcgg tggggatcct gacgacgaga gggggaatga
     1621 cctcgcacgc gtccgtcgtc gcgcgcggaa tgggcaaaac ctgcgtcgtc ggcgcgggcg
     1681 agatcaccgt caacgagacg aagggcgagc ttcgggcgcg aaacctgtcg gcgaaagagg
     1741 gcgacttcct gtccgtcgac gggacggcag gagaggtcat tctcgggaag ctcccgacac
     1801 agccgtcgga gatcctccag gtactgatcg aggggtctct ggcgccggag aaggcgccgc
     1861 tcttcggcgc gttcacgcgc ctgctcgagt gggccgatcg cgcgcgaag
//
LOCUS       DASWAV010000118         5576 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U2665
            k141_206898, whole genome shotgun sequence.
ACCESSION   DASWAV010000118 DASWAV010000000
VERSION     DASWAV010000118.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960316
            Sequence Read Archive: SRR10260007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 5576)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5576)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:47:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,929
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5576
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_206898"
                     /isolate="SMAG_U2665"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.36444 N 132.31889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..927
                     /locus_tag="VF958_02435"
     CDS             <1..927
                     /locus_tag="VF958_02435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011553448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HEX9147998.1"
                     /translation="TLTEGKPRLVTTEAAEGFREDEVLALAAGLERSSEHPLAAAIVR
                     AAADRSLEIPSAEGFESVTGRGVRGRVAGRNVALGNRVLFDDRRIALGSLEQRAEGLR
                     REGQTVMFLAVDGKPVAILGVADPIKATTREAARRLRADGVRIVMLTGDNRTTAEAVA
                     RELGIDEMKAEVLPEEKVGAVKELQNAGHRVAMAGDGINDAPALAQADVGIAMGTGTD
                     VAMESADVTLVKGDLRGISRARSLSRATMRNIRQNLFFAFVYNTIGVPIAAGVLYPFF
                     GILLSPVIAAAAMSVSSVSVIANALRLRRVTL"
     gene            complement(907..1137)
                     /locus_tag="VF958_02440"
     CDS             complement(907..1137)
                     /locus_tag="VF958_02440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013261041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2892 domain-containing protein"
                     /protein_id="HEX9147999.1"
                     /translation="MTMTLNEWLRGIAGTFVLLSVALGWFVHPAFFLFTAFVGANLLQ
                     SAFTKWCPMMVLLKRLGVREPGADTGSERHAA"
     gene            complement(1134..4400)
                     /locus_tag="VF958_02445"
     CDS             complement(1134..4400)
                     /locus_tag="VF958_02445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020677660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HEX9148000.1"
                     /translation="MTDGDDLARGPRPLGFAGRLARAFIESKLTPLLVIFALGLGALA
                     VLMTPREEEPQIKVPMFDVFVAAPGRSVVEVERQVIAPLERELWSVPGVEYVYSTSSP
                     DGGLVIVRFRVGVDPDRAVVRVRSKVDSLLDRWPRDLAPPLVKPRSIDDVPIWALTLS
                     SRTQDPATLRQLAAEIENEIKAIPEVSDTALIGGLRREFRVELDPARLAARGLAPSQV
                     SAAIVAANRRVEAGPLVSNDREISVEAGGFVRSAAELGSVVVAVQAGAPVHLSEVARI
                     EDGPERPPVYVTHGEPRVPGRSPAVTLAVSKRRGANAITVVHAIERKLDLLRPKLVGS
                     DVAMTVTRDYGETASHKSNELLKHMALATLSVALLMALALGRREAGVVLLAIPMTLAL
                     TLFVFWILGYTLNRITLFALIFSIGILVDDAIVVVENVVRHLRQPEAEGRPLAEIAVE
                     ATDEVGNPTILATFAVIAAIVPMGFVGGLMGPYMRPIPVGASAAMLFSLAVAFAVSPW
                     AARRLFRGVPAGSAGHGAARGALGRLYRRAMTPLVTEGRGAARVLFFSVIAALLLAAV
                     ALVPLGVVHVKMLPFDNKNEFQVVVDFPEGTTLETTNRALGEMADSLTGLPEVVGTQT
                     YAGTSAPYNFNGLVRHYFLRSGSNQGDVQVALAPKSERRRSSHEIAKAARPIVTAIGR
                     RYDARVKVAEVPPGPPVLQTLVAEIYGPDDAGRRGVAQQVKDLFARTEGVVDVDWYVE
                     SPREKAVYRLDREKAGIAGIREADVAATLQLALSDDRVDDVQLPGIRERIPLTVTLAD
                     ERRNDLASLSALTVAGSGGRLVPLREIGRFDREPAETSMYHKNLKPVVYVTADVAGAK
                     EAPVYAIAALEKGLSQIRTPDSYRIVTRTASAPFDSTHYGLKWDGEWHVTYEVFRDLG
                     AAFAVVLVLIYVLVVGWFKSFTVPLVIMAPIPLTLVGILPAHALFGVFFTATSMIGFI
                     AGAGIVVRNSIILVDFIELRRREGMALSEAVLDAGEVRFRPMLLTAAAVVVGSSVMLF
                     DPIFQGLAVSMMAGEVAATLLSRAAVPVLYFLLRRSEEKRASAGASRTTSPQPERRFA
                     "
     gene            complement(4397..5452)
                     /locus_tag="VF958_02450"
     CDS             complement(4397..5452)
                     /locus_tag="VF958_02450"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HEX9148001.1"
                     /translation="MKRTPVILAALVVALAGAACARRDARVPPAETVRKLATAPVERR
                     MAPAPIEVEGIVVGRFEAVISSRLAASVATVPAVPGQTVRAGAVLVRLEEQEIRGALA
                     GARAAVEASSVALDLAKKNRERFERLEGRAAAAAVELDRARQEEASARAAAAGAAATL
                     RRAEIDSAQTVLTAPFDAVVVERLVSPGDLAGPGRPLVRIASLSGRRVEAAPAEQDAA
                     ALATGGELEVAVGGRTVTGRIAEIAGAVDSSTRRRTVRVDLPADFEPPLGSFARLILP
                     GPVAPRLLVAESAIVARGGLEIAWVVGADGRASLRYVRTGSRRDGKVEVLSGLEAGER
                     VVLGPPADLQGGTRVTS"
BASE COUNT          959 a         1915 c         1924 g          778 t
ORIGIN      
        1 acgctcacgg agggaaagcc gcggctggtc accacagagg ccgcggaggg gttccgagaa
       61 gacgaggttc tcgctctcgc ggccggcctc gagcgttcga gcgagcatcc tctggcggcg
      121 gctatcgtgc gcgccgcggc ggaccggagc ctggagatcc cgtccgccga gggtttcgag
      181 tcggtgacgg gccggggggt gagagggcgc gttgcagggc gaaacgtcgc gctggggaac
      241 cgggttctct tcgacgatcg gcggatcgcg ctcggttctc tcgaacagcg ggcggaaggc
      301 ctgcgccgcg aaggtcagac cgtcatgttc ctcgcggtgg acgggaagcc cgtggccatc
      361 ctcggcgtcg ccgatccgat caaggcgacg acgcgcgagg cggcgcgccg gcttcgcgcc
      421 gacggagtga ggatcgtgat gctcacggga gacaaccgca cgacggcgga ggcggtggcc
      481 cgggagctcg ggatcgacga gatgaaggcg gaggtcctgc ccgaggaaaa agtcggcgca
      541 gtgaaagagc tccagaacgc gggccaccgg gtcgcaatgg ccggagacgg catcaacgac
      601 gctcctgcgc tcgcccaggc ggacgtcggc atcgcgatgg gcacggggac cgacgtcgca
      661 atggagagcg ccgacgtgac gctcgtcaag ggcgacctgc gcgggatctc gcgcgcgcgg
      721 agcctgtcgc gtgcgacgat gcggaacatc cggcagaacc tgttctttgc gttcgtctac
      781 aacacgatcg gcgtgccgat cgcggccgga gttctctacc cgttcttcgg catcctgctc
      841 tcgccggtca tcgcggcggc cgcgatgagc gtgagctccg tctcggtgat cgccaatgcc
      901 ctgcggctac gccgcgtgac gctctgatcc cgtgtcggcg cctggctcgc ggacgccgag
      961 ccgcttgagc agcaccatca tcggacacca tttcgtgaag gcggactgca gcaggttcgc
     1021 tccgacgaac gcggtgaaga ggaagaatgc cgggtggacg aaccacccca gcgcgaccga
     1081 cagcaggacg aaagtcccgg cgatcccgcg cagccactcg ttcagggtca tggtcatgcg
     1141 aatctccttt ccggttgggg ggaagtcgtt cgagaggcgc ccgcggaggc tcttttctcc
     1201 tcggagcgcc tgagcaggaa atagagcacg gggacagccg cgcgcgagag cagcgtcgcc
     1261 gccacctcgc cggccatcat cgaaacagcg aggccctgga agatcggatc gaacagcatg
     1321 acggacgatc cgacgacgac cgcagccgca gtgagcagca tcggccggaa gcgcacctcg
     1381 ccggcgtcga ggaccgcctc ggagagcgcc atcccctccc tgcgacgcag ctcgatgaag
     1441 tcgacgagga tgatcgaatt tctcacgacg atcccggccc ccgcgatgaa gccgatcatc
     1501 gaggtcgcgg tgaaaaagac tccgaagaga gcgtgcgccg gcagaatgcc gaccagcgtc
     1561 agcgggatcg gcgccatgat gacgagcggc accgtgaagc tcttgaacca gccgacgacc
     1621 agaacgtaga tcagcaccag gacgaccgcg aacgcggccc ccaggtctcg gaagacctcg
     1681 taggtcacgt gccactcccc gtcccacttc aggccgtaat gggtcgagtc gaagggagcg
     1741 gacgccgtac gcgtcacgat ccggtacgag tccggggtgc gaatctgcga cagacctttt
     1801 tcgagcgcgg cgatcgcgta gaccggcgcc tctttcgcgc cggcgacgtc ggccgtgacg
     1861 tacacgacgg gtttcaggtt cttgtggtac atcgaggttt ccgccggctc ccggtcgaag
     1921 cgcccgatct cgcggagcgg caccagcctc cccccgctcc ccgcgacggt cagcgcggaa
     1981 agagaagcga ggtcgttgcg ccgctcgtcg gcaagtgtca ccgtgagcgg gatccgctcg
     2041 cgaatgccgg gcagctgcac gtcgtcgacg cgatcgtccg agagcgcgag ctgcagagtg
     2101 gccgcgacgt ccgcctcgcg aattccggcg atcccggcct tctcgcgatc cagccggtag
     2161 accgctttct cgcggggact ctcgacgtac caatcgacgt cgacgactcc ttcggtccgg
     2221 gcgaagagat ctttgacctg ctgcgccacc ccgcggcggc cggcgtcgtc gggaccgtag
     2281 atctcggcga cgagcgtctg aagcaccggg gggcccggtg gcacctccgc gaccttcacc
     2341 cgggcgtcgt accggcgacc aatcgcggtc acaatcggtc gcgcggcctt ggcaatctcg
     2401 tgcgacgagc ggcgtcgctc gctcttcggc gcgagagcca cctggacgtc tccctggttc
     2461 gacccggagc gcaggaaata gtggcgtacg agaccgttga agttgtaggg agccgaagtg
     2521 ccggcgtagg tctgcgtccc aacgacctcc ggcaggccgg tcaacgagtc cgccatctct
     2581 ccgagcgccc ggttcgtcgt ctcgagcgtc gtgccttccg gaaaatcgac gacgacctga
     2641 aactcgttct tgttatcgaa cggcaacatc ttcacgtgca cgacgccgag aggcacgaga
     2701 gcgacggctg cgagcaggag cgccgcaatc acggagaaga agaggacgcg cgccgctcct
     2761 ctcccctcgg tgacgagcgg agtcatggcc cgccggtaga gtcggccgag cgcgccgcgg
     2821 gccgcgccgt gcccggcaga tccggcaggc acgccccgga agagccgacg cgccgcccac
     2881 ggcgacaccg cgaaggcgac agcgagagaa aagagcattg ccgccgacgc gccgaccggg
     2941 atcggtcgca tgtagggacc catcagcccg ccgacgaatc ccatcggcac gatcgcggcg
     3001 atgaccgcga acgtcgcgag gatcgtcgga ttgccgactt cgtcggtcgc ctcgacggcg
     3061 atctccgcga gcgggcggcc ttccgcttcc ggctggcgca gatggcgcac gacgttctcg
     3121 acgacgacga tcgcgtcgtc gacgaggatg ccgatcgaga agatcagcgc aaagagagtg
     3181 atgcggttga gcgtgtagcc gagaatccag aacacgaaca gcgtgagcgc gagggtcatc
     3241 gggatcgcga gcagtaccac tcccgcctcg cggcggccga gcgcgagcgc catcagcaga
     3301 gcgaccgata gagtggcgag ggccatgtgc ttcaggagct cgttggactt gtgcgacgcg
     3361 gtctcaccgt aatctcgcgt gacggtcatc gcgacgtccg agccgacgag tttcggccgc
     3421 aggaggtcga gctttctttc gatcgcgtga acgaccgtga ttgcattggc gccgcggcgc
     3481 ttgctgaccg cgagcgtgac ggccggagag cgcccgggga cccgcggctc gccgtgggtc
     3541 acgtacaccg gcggcctctc cggaccgtct tcgattcgcg cgacttcgga gaggtggaca
     3601 ggggctccgg cctggacggc gacgacgacg gaaccgagtt ccgcggcgga gcgcacgaag
     3661 ccgccggcct ccactgagat ctcacggtcg ttcgaaacga gcgggcccgc ctcgacccgg
     3721 cggttcgccg cgacgatcgc cgcggatacc tgcgaggggg cgagcccccg cgccgccagg
     3781 cgcgccgggt cgagctcgac gcggaactcc cgccggagac ctccgatcag cgccgtgtcc
     3841 gaaacttccg ggatcgcctt gatctcgttc tcgatctccg ccgcgagctg ccgcagggtc
     3901 gcggggtcct gggtgcggct cgacagcgtc agggcccaga tgggaacgtc gtcgatcgag
     3961 cgcggcttca cgagcggcgg agcgaggtca cgcggccagc ggtcgaggag cgaatcgacc
     4021 ttcgaccgca cgcggacaac ggcgcggtcg ggatccactc cgacgcgaaa ccggacgatg
     4081 accaatccgc catcgggaga ggaggtcgag tagacgtatt cgactccggg gaccgaccag
     4141 agttccctct cgaggggcgc gatcacctgg cgctcgacct cgaccacgct cctgcctggc
     4201 gcggcgacga acacgtcgaa catcggcacc ttgatctgcg gctcctcctc gcggggagtc
     4261 atcaggaccg cgagagctcc gaggccaaga gcaaagatga cgaggagcgg cgtcagcttc
     4321 gattcgatga aagcgcgcgc cagccgcccg gcgaaaccga gcgggcgggg cccccgcgcg
     4381 agatcatctc catcggtcac gaagtcaccc tcgtcccccc ctgaagatcg gccggaggac
     4441 cgaggacgac gcgttcgccc gcctcgagtc ccgagagcac ctcgaccttg ccgtcccggc
     4501 gggaccccgt tcggacgtag cgcagcgacg ctcggccatc ggcgccgacc acccaggcga
     4561 tctcgagacc gcctcgagcg acgatcgcgc tttcggcaac gagcagccgg ggagcgaccg
     4621 gaccgggcag gatgaggcgg gcgaaagagc cgaggggagg ttcgaaatcc gccggcagat
     4681 ccacccgaac cgtccgtcga cgggtcgagg aatcgaccgc gccggcgatc tcggcgatgc
     4741 ggcccgtgac cgtccgacca ccgaccgcga cctcgagctc gccgccggtc gcgagagccg
     4801 ccgcgtcctg ctcggccggc gccgcttcga cgcggcggcc cgacaacgat gcgatccgca
     4861 cgagtggccg tcccggcccg gcgagatcgc cgggggagac cagccgctcg acgaccaccg
     4921 cgtcgaaggg cgccgtcaga accgtctgcg cgctgtcgat ttcggcgcgc cgaagcgtcg
     4981 cggccgcccc tgccgctgcg gctcttgccg aggcttcctc ctgcctcgct cgatcgagct
     5041 ctaccgccgc cgcggcggcc cgtccttcca gccgctcgaa tcgttcacga ttctttttcg
     5101 cgaggtcgag cgcgacgctc gatgcctcga cggcggcccg cgctcccgcg agcgcccccc
     5161 ggatctcctg ttcctcgagc cgcacgagca cggcgcccgc ccgcaccgtc tgaccgggaa
     5221 ccgcgggcac ggtggcaacg gacgccgcga gccggctcga aatgaccgcc tcgaaacgtc
     5281 cgacgacgat accctcgacc tcgatcgggg ccggggccat ccgacgctcg accggagccg
     5341 tcgccagctt ccgaacggtc tcggcgggag gcacccgggc gtcgcgccgg gcacaggctg
     5401 ctcccgcgag ggcgacaacg agcgcagcga gaatcaccgg agttcgcttc atcgggttcc
     5461 accttccgga ggcaggccca gcgcaagcct caaataggcc tcggcgatcg tccgctccga
     5521 gtctgccgtc gaagctccga ggcgtgcgcg caactccgcc gcgcgggctt cgatca
//
LOCUS       DASWAV010000119         3704 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U2665
            k141_645858, whole genome shotgun sequence.
ACCESSION   DASWAV010000119 DASWAV010000000
VERSION     DASWAV010000119.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960316
            Sequence Read Archive: SRR10260007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 3704)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3704)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:47:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,929
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3704
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_645858"
                     /isolate="SMAG_U2665"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.36444 N 132.31889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2550
                     /gene="gyrA"
                     /locus_tag="VF958_02455"
     CDS             <1..2550
                     /gene="gyrA"
                     /locus_tag="VF958_02455"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006000563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HEX9148002.1"
                     /translation="GRALPDVRDGLKPVHRRSLFAMWEQGNLHNKPYKKSARTVGYVL
                     GNYHPHGESAVYDTIVRMAQDFSMRYPLVDGQGNFGSLDGDSPAAMRYTEVRLTRLAA
                     ELIGDDIDKETVDWIPNYDGSTKEPTVLPSKFPNLLVNGSTGIAVGMATNIPPHNLGE
                     VVDATIEMIRRPEATLGQLMKKLPGPDFPTAAFIHGAEGIREAYKTGRGIVQVRARAV
                     IEKAARGEKESIVVTEIPYQVNKAKLVERIAELVNDKKIEGIADLRDETSREGVRIVV
                     ELKRGENAQVVLNQLYALTPMQSTFGIIFLAIVDNQPKILPLADLLRHFIGHRKSVVI
                     RRTQFDLKKAQERAHLLRGLALALANIDLVIKIIRGSRDPKEAKERLISEVSMTRAGL
                     EKFTGVKLGDTPAKGKKAEDVIRLDEIQAQAILDMRLQRLTGLEREKIVAEFVEVLAL
                     IAKLTEILGSEKLVLEIIVDELTEVKKLFGDERRTEIVAEASDIDIEDLIVEEDMVIT
                     VSRGGYIKRSPLSLYRAQRRGGKGRIGATTKEEDVVEHLFVASTHAYILAFTSKGRMH
                     WIKVYEVPQLGPATRGKAIVNLLNLDPNERVVALESTKDFPEDRFLVFATKQGLVKKT
                     ALSAYSNVRSGGIIAINLEDGDELLSVRVTDGTRQIFIGTHLGQGIRFAETDVRPTGR
                     DTTGVWGIALKKDSDFVVEMDVVEEHGHLLTVTEKGYGKRSEASEYRFQGRGGSGVIN
                     IRVTEKNGPVAGIKSVSDADQLLLITQSGMLIRIKVKDIRETGRAAQGVRLIDLDEGD
                     RVVAVAKLAEPEDGDDEPQAALPVPAAGTESEPRSPKLKETDGKTPFLDEE"
     gene            2631..3263
                     /locus_tag="VF958_02460"
     CDS             2631..3263
                     /locus_tag="VF958_02460"
                     /inference="COORDINATES: protein motif:HMM:NF013116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase family protein"
                     /protein_id="HEX9148003.1"
                     /translation="MRFFLDSAVADEARKVREWGLLDGIWLSAGAAASAGVDYRRAVR
                     ELSSLTDGPVCVEPNASDAKGMYKEARELVKLGKDVVVQLPAQPEGMRVARLLAQDQI
                     AVCASACYSAAQALIAAKSNVSYVAPAVGRLDEIGAIGMDLVEQIIRVYDNYGFQTQI
                     VVAELRNPIHVLDAALMGADVVVLSPAVVEQLFQHPLTDEILAGRGRSGQ"
     gene            3260..3526
                     /locus_tag="VF958_02465"
     CDS             3260..3526
                     /locus_tag="VF958_02465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9148004.1"
                     /translation="MKTSPDRVLYLARVLARKLKENLNLAQKADDETVRRAIVRDLTE
                     AFRELDAMEERVRGALAKRKNVSPRDEDFLFAQRLDDELRKHGA"
BASE COUNT          721 a         1217 c         1230 g          536 t
ORIGIN      
        1 ggccgcgcgc tccccgacgt gcgcgacggg ttgaagcccg tgcaccgccg ctccctcttc
       61 gcgatgtggg agcaggggaa cctccacaac aagccttaca agaagtccgc ccgcaccgtc
      121 ggttacgttc tcggcaacta ccacccccac ggcgagtcgg cggtgtacga cacgatcgtc
      181 cggatggcgc aggacttctc gatgcgctac ccgctcgtcg acgggcaggg caacttcggc
      241 tcgctcgacg gcgacagccc cgcggcgatg cgctacaccg aggtacggct gacgcggctc
      301 gccgcggaat tgatcgggga cgacatcgac aaggagacgg tggattggat tcccaactac
      361 gacggctcga cgaaagagcc gaccgtgctc ccctccaagt tcccgaacct gctcgtcaac
      421 ggctcgaccg gcatcgccgt cggcatggcc acgaacatcc ccccgcacaa cctgggcgag
      481 gtcgtcgacg cgacgatcga gatgatccgc cgccccgagg cgacgctcgg gcagctgatg
      541 aagaagctcc ccggcccgga cttccccacc gccgcgttca tccatggcgc cgaaggaatc
      601 cgcgaggctt acaagaccgg ccgcggcatc gtgcaggtga gggcccgggc cgtgatcgag
      661 aaggcggcgc ggggcgagaa ggagtcgatc gtcgtcaccg agatccccta ccaggtcaac
      721 aaggcgaagc tcgtcgagcg gatcgcggag ctcgtcaacg acaagaagat cgagggcatc
      781 gcggacctgc gcgacgagac cagtcgcgag ggggtccgca tcgtcgtgga gttgaagcgc
      841 ggcgagaacg cgcaggtggt tttgaaccag ctctacgcgc tgacgccgat gcagtcgacg
      901 ttcgggatca tcttcctcgc gatcgtcgac aaccagccga agatcctccc gctcgccgac
      961 cttctgcggc acttcatcgg ccaccgcaag tcggtcgtca tccgccgcac gcagttcgac
     1021 ctgaagaagg cgcaggagcg cgcccacctg ctgcgaggcc tcgcgctcgc actcgcgaac
     1081 atcgacctcg tcatcaagat catccggggg tcccgggatc cgaaagaggc gaaggagcgt
     1141 ttgatctcgg aagtctcgat gacgcgcgcc ggcctcgaga agttcacggg cgtgaagctc
     1201 ggggacacgc cggcgaaggg gaagaaagcc gaagacgtca tccggctcga cgaaatccag
     1261 gcccaggcga tcctcgacat gcggctgcag cggctgaccg gcctcgaacg cgagaagatc
     1321 gtcgccgagt tcgtcgaagt gctcgcgctc atcgcgaaac tcaccgagat cctcgggtcc
     1381 gagaaactcg tcctcgagat catcgtggac gagctcaccg aggtgaagaa gctcttcggc
     1441 gacgaacgac gcaccgagat cgtcgcggag gcctccgaca tcgacatcga ggacctcatc
     1501 gtcgaagagg acatggtgat cacggtctcg cgcggcggct acatcaagcg ctctcccctg
     1561 tcgctctacc gcgcgcagcg gcgcggcggc aaggggcgca tcggcgcgac gacgaaggaa
     1621 gaggacgtcg tcgagcacct cttcgtcgcc tccacgcacg cctacatcct cgcgttcacc
     1681 tcgaaggggc ggatgcactg gatcaaggtt tacgaagtcc cgcagctggg tcccgcgacg
     1741 cgcggcaagg cgatcgtgaa cctgttgaac ctcgacccga acgagcgggt cgtggcgctg
     1801 gagtccacga aagacttccc cgaggaccgc ttcctcgtgt tcgccacgaa gcagggcctc
     1861 gtgaagaaga cggcgctctc ggcgtactcg aacgtgcggt cgggcggcat catcgcgatc
     1921 aacctcgagg atggcgacga actcctctcg gtgcgcgtga ccgacggcac ccgccagatc
     1981 ttcatcggca cgcacctcgg ccagggcatc cgcttcgccg agaccgacgt gcgtccgacc
     2041 ggccgcgaca cgacgggggt ctggggcatc gcgttgaaga aagacagcga cttcgtcgtc
     2101 gagatggacg tcgtcgagga gcacggacac ctgctgaccg tgaccgagaa gggctacggg
     2161 aagaggtcgg aagcgtccga gtaccgattc caggggcgcg gcggctcggg cgtcatcaac
     2221 atccgggtca ccgagaagaa cggcccggtc gccgggatca agtcggtcag cgacgcggac
     2281 cagctcctcc tgatcacgca gagcggcatg ctgatccgca tcaaggtcaa ggacatccgc
     2341 gagaccggcc gcgccgcgca gggcgtgaga ctgatcgacc tggacgaggg cgaccgcgtc
     2401 gtcgcggtgg cgaagctcgc ggagccggaa gacggcgacg acgagccgca ggcggcgctg
     2461 ccggtgcccg cagccgggac cgaatccgag ccccggagcc cgaaactcaa agagaccgac
     2521 gggaaaacgc ccttcttaga cgaggagtag agacggcgcg agccctctcc cgccgggaga
     2581 gggctgggtg agggggcacg ccccacgcgc tatagaatcc cgcgccaccc atgcgctttt
     2641 tcctcgacag cgccgtcgcc gacgaggcga gaaaggtccg ggagtgggga ctcctggacg
     2701 gcatctggct ctccgcggga gcggccgcga gcgccggcgt cgactatcgc cgcgcggtcc
     2761 gcgagctctc ctcgttgacg gacgggcccg tctgcgtcga gccgaacgcg agcgacgcga
     2821 agggcatgta caaggaagcc cgcgagctcg tgaagctcgg caaagacgtc gtcgtgcaac
     2881 tgccggcaca accggaggga atgcgcgtcg cgcgcctgct ggcgcaggac cagatcgccg
     2941 tgtgcgcgtc ggcttgctat tccgcggctc aggcgctgat cgcagcgaag tcgaacgtca
     3001 gctacgtcgc gccggccgtc ggccggctcg acgagatcgg cgcgatcggc atggacttgg
     3061 tcgagcagat catccgcgtc tacgacaact acggcttcca gacccagatc gtggtggccg
     3121 agctgcgcaa cccgatccac gtgctcgatg cggcgctcat gggcgcggac gtcgtcgttc
     3181 tctcgccggc cgtcgtcgag cagctcttcc agcatccgct gaccgacgag atcctcgccg
     3241 gccgcgggcg cagcgggcag tgaagacgtc gcccgaccgg gtgctctacc tcgcgcgcgt
     3301 gctcgcgcgg aagctgaagg agaacctgaa cctggcgcag aaggccgacg acgagactgt
     3361 ccgccgcgcg atcgtgcgcg acctgaccga ggcgttccgg gagctcgacg cgatggagga
     3421 acgggtgcgc ggagcgctcg cgaagcgcaa gaacgtctct ccccgcgacg aggattttct
     3481 cttcgcccag cggttggacg acgagctccg aaaacatggg gcatgactca cctaaaacat
     3541 tgatggcaat gagacttata gcaacatggc tccttgaggc ttgcccgaac ggggaagtga
     3601 gactttcgcc ttagccatca acaaattagc gagaaagtca agccgccaac ttcctgcggc
     3661 gatacaattg gctcgatggc gggccaattg gatccgatcg tgct
//
LOCUS       DASWAV010000120         2951 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U2665
            k141_606333, whole genome shotgun sequence.
ACCESSION   DASWAV010000120 DASWAV010000000
VERSION     DASWAV010000120.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960316
            Sequence Read Archive: SRR10260007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2951)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2951)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:47:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,929
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2951
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_606333"
                     /isolate="SMAG_U2665"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.36444 N 132.31889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            23..1282
                     /gene="cls"
                     /locus_tag="VF958_02470"
                     /pseudo
     CDS             23..1282
                     /gene="cls"
                     /locus_tag="VF958_02470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013901869.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008808 - cardiolipin synthase activity
                     [Evidence IEA];
                     GO_process: GO:0032049 - cardiolipin biosynthetic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
     gene            1304..2161
                     /locus_tag="VF958_02475"
     CDS             1304..2161
                     /locus_tag="VF958_02475"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015141275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX9148005.1"
                     /translation="MKTLSGGDGAALAEFLEPYTASSMFLRSNSLLAGLVDRGSPLQG
                     TYVAAVEGERIIAVAAHFWNGILVLQAPELRAIETIARAAVLRSGRELKGLAGPQRQV
                     EMARAALGLSGRATAHDSREDLFELDLAKLEVPRALAAGRLVCRAPRPDELDTVAAWR
                     VDYCVETLGIPNSPDLVATSRAEIARLQQEGSQWIVVDGGRPVSYSAFNARLRDAVQV
                     GGVWTPPELRSRGYGRAVVAGSLLDARSKGARSGILFTGEENRPARAAYEALGFRVVG
                     DYALVIFRD"
     gene            complement(2192..>2951)
                     /locus_tag="VF958_02480"
     CDS             complement(2192..>2951)
                     /locus_tag="VF958_02480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015330896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="isocitrate/isopropylmalate family dehydrogenase"
                     /protein_id="HEX9148006.1"
                     /translation="LYGDILSDVAAQIAGSVGLAGSANIGERCAMFEAIHGSAPRRAG
                     QNLANPSGLLLGAVMMLVHIGQPDVAEKVHNGWLKTIEDGIHTYDIYKEGTSTKKVGT
                     REFGEAVAERLGQRPEKLKPAVYGEAPARASAPAARERKPSERTLVGVDVFVNWPGRA
                     EELERRLRPLVKEGLALTTIANRGIKVWPDGFPETMQTDHWQCRFLAGDSATTHTALA
                     GLLARAAAAGLDYIKTENLYNFDGQRGYSLGQGE"
BASE COUNT          484 a          952 c          999 g          516 t
ORIGIN      
        1 gaagaagtcc gggaggaagg cgatggccgt cgtactggca gtcggcacga cgctcgtgct
       61 gatcctgttg gtgctcaacc tctcttcggg tgaaaagaag atcaagcatc agatcgagcc
      121 gctttacgcg atcgatgatc cgcagtttct ccggtcgatg ggctccctgc tcggcccgcc
      181 ggtcctgggc gggaatcgcg tcgcaacact tctgaacggc gaccagatat ttcccgcgat
      241 gctggaatcc attcgcggcg cgaaggagac gatcgacttc gagacctaca tctactggtc
      301 gggcacggtc gggaaggagt tcgccgacgc cctctccgag cgcgcgcggg ccggggtccg
      361 ggtccacgtc cttctcgatt gggtcgggag cgggaaagcg aaggatgaat acctcgacga
      421 gatgcgcaag gcgggcgcgg aagtcgagaa atatcacccg ctcaagtggt accacctggc
      481 gcggatcaac aaccgcacgc accggaagct cctgatcgtg gacggaaagg tcggattcac
      541 cgggggcgtc gggatcgccg acaagtggtc cggaaacgct caagaccccg atcactggcg
      601 cgactcacac ttccggctcg aggggccggc ggtcgcgcag atgcaggcgt gattcatgga
      661 caactggatg aagacacagt cgaaggttct tcacggcgag gactactttc ctcgactcga
      721 accggtcggg tcgtccggcg cccaggtgtt caagagctcc ccgcgggagg ggagcgagag
      781 cgtgcgtctg atgtacctcc tgtcgatcgc ctccgcccgc cggcggctcc tgatcgccaa
      841 ttcgtatttc gtacccgacg acctcttcgt cgaggcgctc gtggccgcgc gcgggcgggg
      901 tgtcgacgtc gagatcatcg ttcccggaac gaagatcgac tcggagctga cccgaaaagc
      961 ctcccgttcg aggtggggag atcttctcga gaagggggtc gagatctacg agtatcggcc
     1021 cacgatgtac cactgcaagg tgatggtcgt ggacgacctg tgggtatcgg tgggttcgac
     1081 caacttcgac aatcgctcct tccgactgaa cgacgaggcg aatctgaacg tgttcgacgg
     1141 agacttcgcg cgggagcaga ctggagtgtt cgagcgggac aggacccgat cgcaccgcgt
     1201 cacgctcgcg gagtggaagc gccgcccgct ttccgagaag ctccaggaac acctggcggg
     1261 attgctccgg tcacagttgt aaccggctgc cgggaaggtg gacgtgaaga cgctctccgg
     1321 gggggacggg gccgcgctcg cggagttcct ggagccgtac accgcgagct cgatgttcct
     1381 gcggtccaac tcgctcttgg cgggcctcgt cgatcggggc tcgccgttgc agggaacgta
     1441 cgtcgcggcg gtcgaaggcg aaaggatcat cgccgtggcc gcgcacttct ggaacggaat
     1501 cctcgtcctc caggcgcccg agctccgcgc gatcgagacg atcgcgcggg cggccgtcct
     1561 ccgctcggga cgtgagctca agggtctggc cggcccgcaa cggcaagtcg agatggctcg
     1621 cgcggcgctc gggttgtccg ggcgcgcgac ggcgcatgac agccgcgaag accttttcga
     1681 gctcgacctc gcgaagctcg aggtccctcg agccctcgcc gccgggcggc tcgtttgccg
     1741 tgctccgcgt cccgacgagc tcgacaccgt cgctgcgtgg cgcgtcgact attgcgtcga
     1801 aacgcttgga atcccgaatt cgccggacct cgtagcgacg tctcgcgccg agatcgcccg
     1861 ccttcagcag gaagggtcgc agtggatcgt cgtcgacggg ggacgtcccg tttcctactc
     1921 cgcattcaac gcgcggcttc gcgacgcagt ccaggtcggc ggggtgtgga cgccgccgga
     1981 gcttcggagc cgcgggtacg gtcgtgccgt cgtcgccggt tcactcctcg atgcacgttc
     2041 gaagggagcc cgaagcggca tcctcttcac cggcgaagag aatcgaccgg cccgcgccgc
     2101 gtacgaagcg ctgggctttc gagtcgtcgg tgactacgcg ctggtcatct tccgggactg
     2161 agcccccggg accgaggacc ggcgccccgg cctattcccc ctggccgagc gagtagcccc
     2221 gctgtccgtc gaagttgtac aggttctccg tcttgatgta atcgaggccc gcggcggcgg
     2281 cccgggcgag aagtccggcg agcgccgtgt gagtcgtcgc cgagtccccg gcgaggaacc
     2341 ggcactgcca gtgatcggtc tgcatcgttt cgggaaaacc gtccggccag accttgatac
     2401 cgcggttggc gatcgtcgtg agcgcgaggc cctctttcac gagagggcgg agccgccgct
     2461 cgagctcttc ggcgcgtccc ggccagttca cgaagacgtc cactccgacg agagtccgct
     2521 cggagggctt gcgctcccgc gcggccggtg cggatgcccg cgccggcgcc tcgccataga
     2581 ccgccggctt cagcttctcg ggccgctggc cgagtcgctc ggcgaccgcc tctccgaatt
     2641 cgcgggttcc cactttcttc gtgctcgtcc cctctttata gatgtcgtaa gtgtgaattc
     2701 cgtcctcgat cgtcttgagc cacccgttgt gaactttctc ggcgacgtcc ggctgcccga
     2761 tgtgcacgag catcatcacc gcgccgagaa ggagacccga cgggttggcg aggttctggc
     2821 cggcacgtcg cggcgcggag ccatggatcg cctcgaacat cgcgcagcgc tccccgatgt
     2881 tggcggaccc ggcgagcccg accgagccgg cgatctgcgc cgcgacgtcc gagaggatgt
     2941 cgccatagag g
//
LOCUS       DASWAV010000121         2572 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U2665
            k141_687361, whole genome shotgun sequence.
ACCESSION   DASWAV010000121 DASWAV010000000
VERSION     DASWAV010000121.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960316
            Sequence Read Archive: SRR10260007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2572)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2572)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:47:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,929
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2572
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_687361"
                     /isolate="SMAG_U2665"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.36444 N 132.31889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..594
                     /locus_tag="VF958_02485"
     CDS             <1..594
                     /locus_tag="VF958_02485"
                     /inference="COORDINATES: protein motif:HMM:NF012503.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016830 - carbon-carbon lyase activity
                     [Evidence IEA];
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-dependent decarboxylase"
                     /protein_id="HEX9148007.1"
                     /translation="RKVMKGIERSDSIVLDPHKSLFLPYGTGSLLVRDGEALRRAHAL
                     SADYMPTMQADPELLDFNLLSPELSRAWRGLRVWLPVAMHGIGPFRRNLEEKLELTRW
                     ATDELRRVPGIEILAEPQLSIVAFRLARPDLDGDAVNALNRRFLDAINARRRVYLTGT
                     MLGSRYALRICVLSFRTHADRMRECLEDVRAAASSVL"
     gene            733..2139
                     /locus_tag="VF958_02490"
     CDS             733..2139
                     /locus_tag="VF958_02490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015755090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HEX9148008.1"
                     /translation="MKTTRIRFLAGLAGLVLAGGLAAAKPPENTFTVPVEYYKLPNGL
                     KVVLSRDTTAPTAVVAVYYNIGFRIEPRDRTGFAHLFEHMMFQGSQNLGKMEFAKLVQ
                     SNGGVFNGSTRFDFTNYFEIVPAHALETNLWAEADRMRGLAITQDNLTNQQGVVKSEV
                     RVNVLNRPYGGFPWLDLPQYANTNWYNAHNFYGDLKDLDAATLDDVKKFFTTYYSPNN
                     AVLVVVGDFDPAQTRGWIEKYFSAIPASPRPPAPDVSEPRQEKEKRASKTDPLAPRPA
                     LAIGYQMPKRNTPEYYAMGLLDQILLQGKDSRLYEALVQKSGLTGDVNGGINLLGNMH
                     NINGPTLWIVSLFHDADKTPDAILKVLDAEIEKLRTTPVDAATLDLAIVKTRSDIYDS
                     MEGLFGFGRADLLASFALFDDDPGRINHLEEEFRRVTPQILLATAKEYLRPTNRTILT
                     IEPKAEAKPPAAPSEKKS"
     gene            2136..>2572
                     /locus_tag="VF958_02495"
     CDS             2136..>2572
                     /locus_tag="VF958_02495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HEX9148009.1"
                     /translation="MKTKKAAIAFVLALCAGGAFGQKQTPPPPAAPKNFEAPAATKFR
                     LENGLDVTLVPYGTVPKVRVELAVLAGNAYESAKQISLADLAGDLMREGTTTKTASQI
                     SQAAARMGGSLDVAVSVNAVEISGDVLSEFGPEMARLVADVAL"
BASE COUNT          502 a          873 c          776 g          421 t
ORIGIN      
        1 cggaaagtca tgaaggggat cgaacgctcc gactccatcg ttctcgatcc gcacaagagc
       61 ctctttctcc cttatggaac aggttctctt ctcgtgcgag acggagaagc gcttcgccgc
      121 gcccatgctc tctcggcgga ctacatgccg acgatgcagg cggacccgga actcctcgac
      181 ttcaacctcc tgtcgccgga gctgtcgcgg gcctggcgcg gccttcgggt gtggctaccg
      241 gttgcgatgc acggcatcgg accctttcga cgtaacctcg aagagaagct cgagctgacg
      301 cgatgggcga ccgacgagct gcggagagtt ccgggaatcg agatcctcgc agagccacag
      361 ctctcgatcg ttgcgttccg gctcgcgcgc ccggatctcg acggcgacgc ggtaaacgct
      421 ctcaaccggc ggttcctcga cgcgatcaac gcgcgaaggc gcgtctacct gaccgggacg
      481 atgctcggtt cgcgctacgc gctccgaatc tgcgtcctct cgttccgcac gcacgcggac
      541 cggatgcgcg aatgcctgga ggacgtccgg gccgccgcct ccagcgtgct ctgaaaagtc
      601 ccaggaccgc ctggggaaga gggccgacgc cgcgaggatc gagcccggga gtgcgagcag
      661 ggttcattcg ctctggaaaa cctttccttc gtcgagacgt acgcataaag tcgcatttcc
      721 tcaggaggat cgatgaagac cacccgaatt cgcttcctgg ccggtctggc cgggctcgtt
      781 ctcgccggcg gcctcgcggc cgcgaaacct ccggaaaaca ccttcacggt ccccgtcgag
      841 tactacaagc tgccgaacgg cttgaaggtc gtcctctccc gggacaccac ggctccgacg
      901 gcggtcgtcg cggtttacta caacatcggg tttcgcatcg agccgcgtga ccggaccggg
      961 ttcgcccatc tcttcgagca catgatgttc cagggctcgc agaacctggg gaagatggag
     1021 ttcgcgaagc tcgtgcagtc gaacggcggc gtgttcaacg gctcgacccg gttcgacttc
     1081 acgaactact tcgagatcgt gcccgcgcac gcgctcgaga cgaacctctg ggccgaagcc
     1141 gaccggatgc gcggcctggc aattacacag gacaacctca cgaaccagca gggggtcgtg
     1201 aagagcgagg tgcgggtgaa cgtcctgaac cggccttacg gcggctttcc gtggctcgac
     1261 cttccccagt acgccaatac gaactggtac aacgcgcaca acttctacgg cgatctgaag
     1321 gacctcgacg cggcaacgct cgacgacgtc aagaagttct tcacgaccta ctactcgccg
     1381 aacaacgccg tactcgtcgt ggtcggagac ttcgacccgg cccagactcg cggttggatc
     1441 gagaaatact tctcggcaat cccggcatcc ccgcgaccgc ccgctccgga cgtatcggaa
     1501 ccgcgccagg agaaggagaa gcgcgcgtcg aagacggatc cgctcgcgcc gcggccggcg
     1561 ctcgccatcg gctaccagat gccgaagcgg aacacgcccg agtactacgc catgggcttg
     1621 ctcgaccaga tccttctgca gggaaaggac agccgcctct acgaagctct cgtccagaag
     1681 agcgggctca ccggagacgt caatggagga atcaaccttc tcggcaacat gcacaacatc
     1741 aacggcccga cgctctggat cgtgtcgctc ttccacgacg cggacaagac tcccgacgcg
     1801 atcctgaagg tcctcgacgc ggagatcgag aagctgcgca cgactccggt cgacgccgcg
     1861 actctcgatc tcgcgatcgt caagacgcga tccgacatct atgactcgat ggaaggcctg
     1921 ttcggcttcg gccgcgccga tctgctcgcg agctttgctc tcttcgacga cgatccgggc
     1981 aggatcaacc acctcgaaga ggaattccgg cgcgtgacgc cgcagatcct gctcgcgacc
     2041 gcaaaagagt accttcgccc caccaaccgc acgatcctga cgatcgagcc gaaggccgag
     2101 gcgaagcccc ccgccgcccc ctcggagaag aaatcatgaa gacgaaaaaa gccgccatcg
     2161 cgtttgtcct cgccctctgc gcgggcggcg ccttcgggca gaagcagact ccgccgcctc
     2221 cggcggctcc gaagaacttc gaggcgccgg cggcaacgaa gttccgtctg gagaacggac
     2281 tcgatgtcac gctcgtcccc tacgggacgg tcccgaaagt gcgcgtggag ctcgcggtgc
     2341 tcgccggcaa cgcctacgag agcgcgaagc agatctcgct cgccgatctg gccggagacc
     2401 tcatgcgcga ggggacgacc acgaaaaccg cgagccagat ctctcaggcg gccgcgcgca
     2461 tggggggctc tctcgacgtc gccgtcagcg tcaacgccgt cgaaatctcc ggcgacgtgc
     2521 tctccgaatt cgggcccgag atggcccgtc tcgtcgcaga cgtcgcactc aa
//
LOCUS       DASWAV010000122         3305 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U2665
            k141_167050, whole genome shotgun sequence.
ACCESSION   DASWAV010000122 DASWAV010000000
VERSION     DASWAV010000122.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960316
            Sequence Read Archive: SRR10260007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 3305)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3305)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:47:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,929
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3305
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_167050"
                     /isolate="SMAG_U2665"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.36444 N 132.31889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2166
                     /locus_tag="VF958_02500"
     CDS             <1..2166
                     /locus_tag="VF958_02500"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:NF024703.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HEX9148010.1"
                     /translation="GRLDGCVERSEGSWLIEEFKSTHLPSEGLRLSGYGIDRDRRQLL
                     GYCYLWRRLGHPHVSGALVYVDIATDEEISLAVPYDEDEFDREVEARLQRLLRIWKGQ
                     LAVRRRKTEAAQRLPFPHAAPRPIQQEVMAAVDHAVRSREILIAEAPTGSGKTAAALH
                     PALAAGLESGRQVVFLTSKTLQQRMAVSALTAMNQRTFHTLQVRAKEKMCANDRILCH
                     EEFCRFAKNYPEKMARSNILGRLRDSYSHHDPDTVFEEARREEVCPFEVQLELAERAD
                     AIVADYNYVFEPGVALRHLSGDDLTDAILIVDEAHNLPDRARQIFSPQILEGDLRALF
                     NRLALQPGDLFADLSASVADLIEILEGAATDLADGEAIAEIAPPAEEIYAWRAGWEPR
                     LVRYLDWKRDTKLALPDDPVLDLHFGVTRFAGVLNLFGPGFTCVVERRTDGLRLALVC
                     LDPARAVGPVFRAASSAVLMSATLAPHAAVERLLGLPADRTSVISLPPPFPRENRKVL
                     IVPQVRTTFAARERNYGRIAALLGEMSDAHSGNDLVLFPSYRFLAEVAQLMPPTRARL
                     LLQRADLSDFERQRVLQVLASPPPEGILLFAVSGGMYAEGVDYPGEILSGVFVISPAL
                     PQVSFERELLRQYFEEQEEAGFDYAYLQPGMTRVVQAAGRLIRSETDRGVIALLCHRF
                     LEEPYVSRLPRDWYLETPDELVSRRPAEEIREFFESYGR"
     gene            2156..2725
                     /locus_tag="VF958_02505"
     CDS             2156..2725
                     /locus_tag="VF958_02505"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEX9148011.1"
                     /translation="MAGKRKERSVRKKPPTDWGSRSDPYRDRSLPRVTVDAWIEDGRG
                     RILLVRRGRPPFEGRWSLPGGFCEWGETTEAACARESREETGLKVRIGGVLGVYSDPK
                     RDPRGHTISVLYEAKPIGGSAKGGDDAADARWFTTSQLLSAELAFDHAEIVREQLARR
                     RRVRSTSSGLRARRPGVRSRRARPGGARG"
     gene            complement(2655..>3305)
                     /locus_tag="VF958_02510"
     CDS             complement(2655..>3305)
                     /locus_tag="VF958_02510"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF015597.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0009691 - cytokinin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LOG family protein"
                     /protein_id="HEX9148012.1"
                     /translation="ELHFYRVAVFGSARIRTGDPEYAAVRELAGRLTQLGCDIVTGGG
                     PGLMEAANEGAASSRGTRKTRSFGLTISVPYEKTNPYLDTVTAHRTFFSRLHHFVRMS
                     QAFVIFPGGVGTSLELLMVWQLLQVGFLTERPVVLVGEMWLGLTDWMRREMVSRSLVN
                     ASDLDLVTLAPSIDEAVAKIESHKLKFDAAREQIILERRRAARAANAPPAVEPVIR"
BASE COUNT          530 a         1120 c         1096 g          559 t
ORIGIN      
        1 ggacggctcg acggttgcgt cgagcggtcc gagggctcgt ggctgatcga ggagttcaag
       61 tcgactcacc tcccgtcgga gggattgagg ctgtcgggct acggcatcga ccgcgaccgg
      121 aggcaattgc tcggctactg ctatctctgg cgccgtctgg ggcatcccca tgtctccggc
      181 gcgctcgttt acgtcgacat cgcgacggac gaggaaatct cgctcgccgt cccctacgac
      241 gaggacgagt tcgaccgcga ggtggaggcc cgactccagc ggctcctccg gatctggaaa
      301 ggtcagctgg cggtgcgccg ccgcaagacc gaggccgcgc agcgccttcc ctttcctcac
      361 gcagctccgc gcccgattca gcaggaggtg atggcggcgg tggatcacgc cgtccgttcg
      421 cgggagatcc tcatcgccga agcgcccacc ggctcgggca aaacggcggc agcgctccac
      481 ccggcccttg ccgccgggct cgagagcggc cgccaggtcg tcttcctcac ctcgaagact
      541 ttgcagcaga ggatggccgt ctcggcgctc acggcgatga accagcggac cttccacacc
      601 ctgcaggtcc gggcgaagga gaagatgtgc gcgaacgacc ggatcctctg ccacgaggag
      661 ttctgccgct tcgcgaagaa ctacccggaa aagatggcgc ggtcgaacat cctcgggcga
      721 ttgcgcgact cctactcgca tcacgatccg gacacggtct tcgaagaggc gcgccgcgag
      781 gaggtgtgcc ccttcgaggt gcagctcgag ctcgcggaac gcgcggacgc gatcgtcgcg
      841 gactacaact acgtcttcga gcccggcgtc gcgctgcgcc atctgtcggg ggacgacctg
      901 accgacgcga tcctgatcgt cgacgaggcc cacaaccttc ccgaccgagc gcgccagatc
      961 ttttcgccgc agatcctcga aggcgacctc cgtgcactct tcaatcggct ggcgctccag
     1021 cccggggacc tgttcgcgga cctctcggcg agcgttgcag acctgatcga gatcctggag
     1081 ggcgccgcga cggatcttgc cgacggggag gcgatcgccg agatcgcccc tcccgccgag
     1141 gaaatttacg cgtggagggc cggttgggag ccccggctgg ttcgatatct cgactggaag
     1201 agggacacga agctcgcgct gcccgacgac cccgtgctcg acctgcattt cggagtcacg
     1261 aggttcgccg gggttctgaa cctcttcggt cccggcttca cctgcgtcgt cgagcgccgg
     1321 accgacgggc tccgtctcgc tctcgtctgt ctggacccgg cgcgcgccgt gggaccggtc
     1381 ttccgcgcgg cgtcgtccgc cgtcctcatg tcggcgacgc tcgcgccgca tgccgcagtc
     1441 gaacgccttc tgggacttcc tgccgaccgg acctcggtga tctctcttcc gccgccgttc
     1501 ccgcgtgaga atcgcaaggt cctgatcgtc ccccaggttc gcacgacgtt cgccgctcgc
     1561 gaacggaact acggccgcat cgccgcactt ctcggggaga tgtcggatgc gcactccggc
     1621 aatgatctgg tcctctttcc ctcgtaccgg ttccttgccg aggtcgctca attgatgccg
     1681 ccgacccggg cgcgcctgct cctccaacgc gcggatctct ccgatttcga gcgccagcga
     1741 gtcctccagg tgctcgcgtc ccccccgccg gaggggattc tcctcttcgc cgtctcgggc
     1801 ggcatgtacg cggagggcgt cgactacccg ggcgaaatcc tctcgggcgt gttcgtcata
     1861 tcgcccgcgc ttccccaggt gtccttcgag cgggagctgc tgcgtcagta tttcgaggaa
     1921 caggaagagg cgggcttcga ctacgcctac ctccagcccg gaatgacgcg cgtcgtccag
     1981 gccgccggtc gtctgatccg aagcgagacg gaccgcggcg tcattgcgct cctctgccat
     2041 cgcttcctgg aggagcccta cgtcagccgc ctgccgcgcg actggtacct ggagaccccg
     2101 gacgagctcg tgtcgcggcg tcccgccgag gagataaggg agttcttcga gagctatggc
     2161 cggtaaacgc aaggagaggt cggtcaggaa gaagcctccg accgattggg gttcgcgctc
     2221 ggacccctac cgcgacaggt cgctcccgcg agtgaccgtc gacgcctgga tcgaggacgg
     2281 ccgcggacgg attctgctcg tgcggcgcgg ccgtccgccg ttcgaaggcc gctggtcact
     2341 ccccggcggt ttctgcgagt ggggagaaac gacggaagcc gcgtgcgccc gggaatcgcg
     2401 ggaggaaacc ggcttgaaag tacggatcgg cggggtgctc ggggtgtact cggatccgaa
     2461 acgcgatccg cgcggccaca ccatcagcgt cttgtacgag gcgaaaccga tcgggggatc
     2521 ggcgaagggc ggggacgacg ccgcggacgc gcgatggttc accacctcgc agctcctgag
     2581 cgccgagctc gcgttcgacc acgcagagat cgtccgcgaa cagctcgccc gccgccggcg
     2641 cgttcgatcg acttctagcg gattacgggc tcgacggccg ggggtgcgtt cgcggcgcgc
     2701 gcggcccggc ggcgctcgag gatgatctgc tcgcgcgcag cgtcgaactt cagcttgtga
     2761 ctctcgatct tcgcgaccgc ctcgtcgatc gagggagcga gtgtgacgag gtcgagatcg
     2821 ctcgcgttca cgaggctgcg tgagaccatc tcgcgacgca tccagtcggt caacccgagc
     2881 cacatttcac cgacgagaac cacgggtcgt tccgtcagga acccgacctg gaggagctgc
     2941 cagaccatca agagctccag gctcgttccg acgccgcccg ggaagatgac gaatgcctgc
     3001 gacatccgga cgaagtggtg gaggcgcgaa aagaacgtgc ggtgagccgt gaccgtgtcg
     3061 agatagggat tcgtcttttc gtaaggaacc gaaatcgtca gcccgaagga tcgggtcttt
     3121 cgagtgcctc gggaagaggc ggcgccctcg ttggccgcct ccatcaaccc cgggcctccc
     3181 ccggtcacga tgtcgcaacc gagctgggtg agccggcctg cgagttcccg gaccgctgcg
     3241 tactcggggt cgccggtgcg aatgcgcgcg cttccgaaaa ccgccacgcg gtagaagtgc
     3301 aactc
//
LOCUS       DASWAV010000123         1956 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U2665
            k141_406868, whole genome shotgun sequence.
ACCESSION   DASWAV010000123 DASWAV010000000
VERSION     DASWAV010000123.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960316
            Sequence Read Archive: SRR10260007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 1956)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1956)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:47:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,929
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1956
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_406868"
                     /isolate="SMAG_U2665"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.36444 N 132.31889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..921
                     /locus_tag="VF958_02515"
     CDS             <1..921
                     /locus_tag="VF958_02515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011522589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase C-terminal domain-containing
                     protein"
                     /protein_id="HEX9148013.1"
                     /translation="EGVLAECKGKGGVFKVTHNLQRLYGKERVFNSPLAEANIVGRAI
                     GMALRGLKPVVEIQFYDYIWPAYMQIRSELATMRWRSGNTWTCPVVVRVAVGGYIGGG
                     AIYHSQSGAVLMTHIPGLRVVMPSNALDANGLLRTAIRCDDPVMFLEHKHLYRQTHNK
                     SPYPGPDYMVPFGKASVVQEGKDVTIVTYGATVYRSWQAAKAFTEEAGKSVEILDLRS
                     LSPYDWEAIAQSVKKTGRAIVVYEDPISRGYGAEIAARISSELFPWLDAPVERVAATD
                     TFVAYQPQVEDFILPQAAHVRKALEKVIGF"
     gene            1087..1305
                     /locus_tag="VF958_02520"
     CDS             1087..1305
                     /locus_tag="VF958_02520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9148014.1"
                     /translation="MRREFQQLQLTVESVHANHRALRRCHGCGNYERPFVEMDGIIRC
                     ERCAKKGEEDDESEKKDLGSVGEFGRIL"
     gene            1346..>1956
                     /locus_tag="VF958_02525"
     CDS             1346..>1956
                     /locus_tag="VF958_02525"
                     /inference="COORDINATES: protein motif:HMM:NF012402.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HEX9148015.1"
                     /translation="MKADHRAFLLARRVFSPGFFSATFRAPALAREVLPGQFVMLEVA
                     GRLRPYLRRAYSVADADPATGEVEFLVKTIGPGTAALEDLAEGSGASLLGPLGNGFSL
                     AGLPSAARVAIVAGGIGAAPFPLLLRAMGEAGVSADLFLGGRNARELGFRDRFAGLVP
                     GETLLSTDDGSLGEKGFVTDAFARRAAATPYSRVYACGPMPMFV"
BASE COUNT          318 a          645 c          648 g          345 t
ORIGIN      
        1 gagggggtcc tcgccgagtg caaggggaaa gggggcgtct tcaaggtcac gcacaacctt
       61 cagcggctct acggcaagga gcgggtcttc aactcgcccc tggccgaagc gaacatcgtg
      121 gggcgcgcca tcggcatggc gctccggggt ttgaagccgg tcgtcgagat ccagttctac
      181 gactacatct ggcccgcata catgcagatc cggagcgagc tcgcgacgat gcgctggcgc
      241 tcggggaaca cgtggacctg tcccgtcgtc gtgcgcgtcg cggtgggcgg atacatcggc
      301 ggcggcgcga tttaccactc gcaatccgga gcggtcctga tgacgcacat ccccggcctg
      361 cgcgtcgtca tgccgtcgaa cgcgctcgat gcgaacggcc tccttcgaac cgccatccgc
      421 tgcgacgatc cggtgatgtt cctcgagcac aaacacctct accgccagac gcacaacaaa
      481 tcgccgtatc cgggcccgga ctacatggtt ccgttcggca aggcgtcggt cgtccaggag
      541 gggaaggacg tcacgatcgt gacttacggc gcgacggtct accgctcgtg gcaggccgcg
      601 aaggcgttca ccgaggaggc cgggaagagc gtcgagatcc tcgacctgcg aagtctctcc
      661 ccctacgact gggaggcgat tgcgcagtcg gtcaagaaga ccggtcgcgc gatcgtcgtc
      721 tacgaggacc cgatctcgcg gggatacggt gcggagatcg cggcgcgcat ctcctccgag
      781 ctcttcccgt ggctcgacgc gcccgtcgag cgggtcgccg cgaccgacac tttcgtcgcc
      841 taccagccgc aggtggagga cttcatcctt ccccaggcgg cgcacgttcg gaaggcgctc
      901 gagaaggtga tcggcttcta gccggacgcc gactgacctc gcgatcggac aacttcctcc
      961 tccgggcgac aacttttgaa catgacctcg ccaccaagtc ttttcttttc agcagttgcc
     1021 agatggtacg cagtctgctc tctcggacaa cgtcactaag ggaagtgaca gaagaaggtg
     1081 agaccaatga gaagggagtt ccagcagctt cagttgaccg tcgagtcggt tcacgcgaac
     1141 catcgcgctc tgcgccggtg ccacgggtgc ggcaactacg aacgcccctt cgtcgagatg
     1201 gatggaatca tccgctgcga gcggtgcgcg aagaagggcg aggaggacga cgagtcggag
     1261 aagaaggacc tcgggtccgt cggcgaattc ggccggatcc tgtaacggcg tgtcggctcc
     1321 cgggcggagc tgatagcttt cgcccgtgaa ggccgatcat cgggcgtttc tcctcgcccg
     1381 gcgcgtcttc tctcccggat ttttctccgc aacattccga gctcccgctc tcgcgcgcga
     1441 agtccttccc ggacagttcg tgatgctcga ggtcgcggga cgtttgcgcc cctacctgcg
     1501 ccgggcgtac tcggtcgcgg acgcagatcc ggcaaccgga gaggtcgagt tcctcgtcaa
     1561 gacgatcggg ccgggcacgg cggcgctcga ggacctggcg gagggatccg gcgcgtctct
     1621 cctcggcccg ctcggcaacg ggttctcgct cgccgggctt ccttctgccg cgcgggtcgc
     1681 gatcgtcgcg ggcggaatcg gcgcggcgcc atttccgctg ctgctccgcg cgatggggga
     1741 ggcgggcgtg tccgccgatc tcttcctggg aggccgcaac gcgcgcgagc tcggctttcg
     1801 cgaccgcttc gcgggcctgg tcccgggcga aaccctccta tcgaccgacg acggctcgct
     1861 cggggagaag ggttttgtca ccgacgcctt cgcccgccgc gccgcggcaa ccccgtactc
     1921 gcgggtctac gcgtgcggcc cgatgccgat gttcgt
//
LOCUS       DASWAV010000124         3226 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U2665
            k141_285934, whole genome shotgun sequence.
ACCESSION   DASWAV010000124 DASWAV010000000
VERSION     DASWAV010000124.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960316
            Sequence Read Archive: SRR10260007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 3226)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3226)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:47:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,929
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3226
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_285934"
                     /isolate="SMAG_U2665"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.36444 N 132.31889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..942)
                     /gene="nusA"
                     /locus_tag="VF958_02530"
     CDS             complement(<1..942)
                     /gene="nusA"
                     /locus_tag="VF958_02530"
                     /inference="COORDINATES: protein motif:HMM:TIGR01953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusA"
                     /protein_id="HEX9148016.1"
                     /translation="MNIDPSVIRQVSREKDIDAEKMFGALEEAMSSAARKFYHDKAMV
                     TLIDRQTGAMTVYSPRTVVEELDTESEEPPPQVLLDEAQDLVKKHGTRGEVRVFRPGA
                     EGEPPVEVDDVRLGDEVRVYREAMGLGRIAAQTAKQVLYQKVREAERDNIYNEYYPKV
                     GELMSGTVKRFERGDMIVDLGKTEAIIPRDQQSRAERYGQGERIRSILYDVHKNPKGP
                     QLVLSRTDPRLLIRLFEMEVPEIYDGTVVIKGAVRQPGERAKVAVRSRERDVDPVGAC
                     VGMRGSRVQSIMRELHGEKIDVILYSDDLITFAQNALA"
     gene            complement(973..1536)
                     /gene="rimP"
                     /locus_tag="VF958_02535"
     CDS             complement(973..1536)
                     /gene="rimP"
                     /locus_tag="VF958_02535"
                     /inference="COORDINATES: protein motif:HMM:NF014617.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000028 - ribosomal small subunit assembly
                     [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0042274 - ribosomal small subunit
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HEX9148017.1"
                     /translation="MLPLGPETTEEIAVIAREKGCRLLAIESSGGGRASVLRLVLERA
                     DGSGVSVEDCEAVSREVSTLLDASDEISHGYTLEVSSAGLDRKLYTIDDARRFIGRRI
                     RVRTDLPVVPEASDRPGVATESRDPAAAENRDRAAAGSRNLRGRLETVEADVLRVVDE
                     ENRKTYNVRFGDIRQARLEYEWPERRR"
     gene            1765..2748
                     /locus_tag="VF958_02540"
     CDS             1765..2748
                     /locus_tag="VF958_02540"
                     /inference="COORDINATES: protein motif:HMM:NF024503.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HEX9148018.1"
                     /translation="MFEIVEKGQKPFPVERAVLLSIVAHILLLVAIISAPPTFASKHP
                     ERGLLAAFIPKDSDQEKIPIVFRSAPGPARANPRRSDLSDADRRAGGGDRSKPRSSTP
                     FIEPRPGIEGLQPGRRGREPQTAQLQGVPLPRRGDSTAGESSNERPAIFGPGGEKSDS
                     NVAAGSEGRSGATGGRLSSLDQAIREAARSAAAGGGQEGGGFPNAEGGFVDSGPISFD
                     TTWYDWGPYADEMVRRIKLHWEIPELARLGWKGKLTIRFFILADGRVADAKIIRGSGI
                     PPFDFAALQAILKSSPFRPLPPDLGEPREGVTVTFFYNLRPEKEGRPEGTK"
     gene            2745..3188
                     /locus_tag="VF958_02545"
     CDS             2745..3188
                     /locus_tag="VF958_02545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004340039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HEX9148019.1"
                     /translation="MNDPGQIRTALESARTIAVVGCSPRPDRPSHAIARYLMEQGYHV
                     IPVNPGHRTILQETCYRSLGEIPEEVGIDIVDVFRRSDQVGAIADAAIARGVGFFFMQ
                     QGVLDAEAARRLEAAGIPVAMDRCILVEHASRALPPRNFEARRQR"
BASE COUNT          505 a         1110 c         1056 g          555 t
ORIGIN      
        1 agcgagggca ttctgcgcga aggtgatgag gtcgtcggaa tagaggatga cgtcgatttt
       61 ttcgccgtgg agctcccgca tgatcgactg cacgcggctt ccgcgcattc cgacgcaggc
      121 gccgaccgga tcgacgtcgc gctcgcgcga ccgcacagcg actttggcgc gctcgccggg
      181 ctggcggact gcgcccttga tgaccacggt tccgtcgtag atctcgggca cttccatctc
      241 gaagaggcgg atgagcaggc ggggatcggt gcgggagagc acgagctgcg gtcctttcgg
      301 gttcttatgc acgtcgtaga ggatcgaccg gatccgctcg ccctggccgt accgctccgc
      361 acgcgactgt tgatcgcgcg ggatgatcgc ttccgtcttt ccgaggtcga cgatcatgtc
      421 gccccgctcg aagcgcttca cggtgccgct catgagctcc ccgaccttcg ggtagtactc
      481 gttgtagata ttgtcgcgct cggcttctcg gaccttctgg tagagcacct gcttggcggt
      541 ctgcgcggcg atgcggccca ggcccatcgc ttcccggtag acgcgaactt cgtctccgag
      601 gcggacgtcg tcgacttcga cgggcggctc gccctcggct cccgggcgga acactctcac
      661 ttctccccgg gtcccgtgct tcttgacgag atcctgcgcc tcgtcgagga ggacctgcgg
      721 cggcggctcc tcggattccg tgtcgagctc ctcgacgacg gtgcgcggcg agtacacggt
      781 catggcgccg gtctggcggt cgatcagggt caccatcgcc ttgtcgtggt agaactttcg
      841 cgcggccgag gacatggctt cctcgagcgc gccgaacatc ttctcggcgt cgatgtcttt
      901 ttcccgcgag acctgtcgga tcaccgacgg atcgatgttc atctgactgg actgctccta
      961 accgttcgcg tgctacctgc ggcgctccgg ccactcgtac tccaaccgcg cctgccgaat
     1021 atcgccgaaa cggacattat aggttttgcg attctcctca tcaacgacac ggaggacgtc
     1081 tgcctcgacc gtctcgagtc ttccgcgaag attgcggctg cccgcggctg cgcgatcgcg
     1141 gttctccgcg gccgcgggat cgcggctctc cgtcgcgacg cccggccggt ccgacgcctc
     1201 cggcacgacc gggagatccg tcctgacccg aatccgccgg ccgatgaacc ggcgcgcgtc
     1261 gtcgatcgtg tagagcttcc ggtcgagccc ggcggaggag acctcgagcg tgtagccgtg
     1321 cgagatctcg tcggaggcat ccaggagcgt cgacacctcg cgcgacaccg cctcgcagtc
     1381 ctcgaccgaa accccgctcc catccgcgcg ctcgagcacg agccggagca cggaagcgcg
     1441 cccccctccc gacgactcga ttgcgagcag acggcatccc ttctcgcgcg caatcacggc
     1501 gatctcctcg gtcgtctcgg gtccgagcgg cagcattcac cctgtctcca ggaacaaaaa
     1561 agcgggcctg cgcccgcctc cacttcatcg agagccgcct ctgtttatca gaaagaacgg
     1621 gctcgccgca acctcccgag cccccgcccg gcccttgatg ccgcgctcgg aagcccggac
     1681 ccggccgggg acgcctcaaa gtgagaatcg gggccgtcgc aggcgtgtta cggctggagg
     1741 gccagcgccc acaagcgaca caacgtgttc gaaatcgtcg agaaggggca gaaaccgttt
     1801 ccggtcgaga gggccgtcct gctctcgatc gtcgctcaca ttctcctgct cgtcgcgatc
     1861 atttcggcgc cgccgacctt cgcctcgaag catcccgagc gcggcctgct cgcggcgttc
     1921 atcccgaagg actccgacca ggaaaagatt ccgatcgtgt tccgctcggc gccgggtccc
     1981 gcgcgggcga acccgcggcg ctccgacctc tccgatgccg accggcgcgc gggagggggg
     2041 gaccgttcga aaccccggtc ctcgacgcct ttcatcgagc cacggccggg catcgagggg
     2101 ctccagcccg gacgccgcgg ccgtgagccc cagacggcgc agctgcaggg tgttccgctt
     2161 ccgcgccgcg gggactccac ggccggcgag tcttctaacg aaaggccggc aatcttcgga
     2221 cccggtgggg aaaagtcgga ctcgaacgtc gccgccgggt cggaggggcg atcgggagcc
     2281 actggaggcc ggttgtcgag cctggatcag gcgatccgcg aggcggcgcg ttccgccgcc
     2341 gccggagggg ggcaagaggg gggaggcttc ccgaacgccg aaggggggtt cgtcgactcc
     2401 ggaccgatct ccttcgacac gacctggtac gactggggcc cctatgccga cgagatggtg
     2461 cggcgcatca agctccactg ggagatcccc gagctcgcgc ggctgggctg gaagggaaag
     2521 ctgacgatcc gcttcttcat cctcgccgac ggccgcgtcg ccgacgcgaa gatcatccgc
     2581 ggctcgggca ttcccccttt cgatttcgcc gcactccagg cgatcctgaa gtcgtcgcct
     2641 ttccggcctc tgccgccgga cctcggcgaa ccgcgcgaag gcgtcaccgt aacctttttc
     2701 tacaatctcc gtcccgagaa ggagggacgg ccggaaggca cgaaatgaat gaccccgggc
     2761 agatccggac ggccctggag agcgcgcgga cgatcgccgt cgtcggctgc tcgcccaggc
     2821 cggatcgacc gtcccacgcg atcgcccggt acctgatgga gcagggctac catgtgattc
     2881 cggtcaaccc gggacaccgg accatcctcc aggaaacgtg ctaccggtcg ctcggcgaga
     2941 taccggagga ggtcgggatc gatatcgtgg acgtcttccg acggtccgac caggtcggag
     3001 ccatcgccga cgcggcgatc gcacgaggtg tcgggttctt cttcatgcag cagggtgtct
     3061 tggacgcgga agccgcccgg cggctcgagg cggccgggat ccccgtcgcc atggaccgct
     3121 gcatcttggt cgagcacgcg agccgggcgt tgcctccgag gaatttcgaa gctcgcaggc
     3181 agcgttaagt cggtgaccgg cagccacgcg gttccagcgg agttgc
//
LOCUS       DASWAV010000125         2413 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U2665
            k141_326201, whole genome shotgun sequence.
ACCESSION   DASWAV010000125 DASWAV010000000
VERSION     DASWAV010000125.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960316
            Sequence Read Archive: SRR10260007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2413)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2413)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:47:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,929
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2413
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_326201"
                     /isolate="SMAG_U2665"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.36444 N 132.31889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..477)
                     /locus_tag="VF958_02550"
     CDS             complement(<1..477)
                     /locus_tag="VF958_02550"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone/menaquinone biosynthesis
                     methyltransferase"
                     /protein_id="HEX9148020.1"
                     /translation="MNKYLSYDEERAPKVREMFSRLAERYDLVNDVMSFGMHRHWKRE
                     TVRLALNGRKERPRLLDLCCGTGDMTFRAEEMKAGRVVGADFTIPMLAVARRRAHSAA
                     VRPDFIAADALCLPFRDGSFDAITVGYGLRNIADPRLALAEMRRVLAPGGRAVVLDF"
     gene            554..>2413
                     /locus_tag="VF958_02555"
     CDS             554..>2413
                     /locus_tag="VF958_02555"
                     /inference="COORDINATES: protein motif:HMM:NF012656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEX9148021.1"
                     /translation="MAVKKGARALNEEESRQVAEEARETEWTAPSFLKEIFLGNYRLD
                     LVHPFPEAPLDRPEFRAFMDRLERFLVEEVDSDRIDREGKVPPQIVERLAELGAFGIK
                     IPKEYGGLGFSQREYTEAIKLVTSQDGNLTTLLSAHQSIGLPQPLKLFGTKEQKKKYF
                     PRLARGAVSAFALTEPDVGSDPANLSTTVEKTPEGDYLLNGEKLWCTNGTIAELYVVM
                     ARHPDTRKISAFIVERDSPGVEVVHRCHFMGLRGIENGVIRFANVRVPKEDLLFAEGR
                     GLKLALVTLNTGRLTLPASCTAGAKRALEICRGWANERVQWGQPIGRHDAIAQLLAEM
                     AATTFAMEAISDLASAMADMGNRDIRLEAAVAKMWNSEEGWRIVDRTMQIRGGRGYET
                     ADSLKARGEAPIAVERMMRDFRINLIFEGSSEIMRLFIAREALDRHLAVAQDLFNKKL
                     TRQQRLATLPKFVRFYSTWYPSRWAGWGRWPRFKSFGPMSGHVRYMERTTRRLSRAIF
                     HLMATHGPKLEKRQALLFRAVDIGADLFAMSAAVSRADGLRRSRAPEAKSALELADVF
                     CRMMRRRITAAFDAIRSNDDVRKYRIARRFLDGEHKWLERGMTPMGGVEEFAAR"
BASE COUNT          422 a          779 c          820 g          392 t
ORIGIN      
        1 gaagtcgagg acgaccgcgc gtcctcccgg cgcgagcacg cggcgcattt ctgcgagcgc
       61 gagccgcggg tcggcgatgt tgcgcagccc gtacccgacg gtgatcgcgt cgaaggaacc
      121 gtcacggaag ggaagacaca gcgcgtccgc cgcgatgaaa tccggtcgga cggctgccga
      181 gtgcgcccgc cgtcgcgcga cggcgagcat ggggatcgtg aagtcggcgc ccaccacgcg
      241 gcccgctttc atctcctccg cgcggaatgt catgtcgccc gtgccgcagc agagatcgag
      301 caaccgcggc cgctctttcc gtccattcag ggcgagccgg actgtctccc gcttccagtg
      361 gcggtgcatc ccgaagctca tgacgtcgtt gacgaggtcg tagcgctcgg cgagccggct
      421 gaacatctcg cgcactttcg gcgcgcgctc ttcgtcgtag gacaggtact tgttcataga
      481 acggggtcag gtctccactt gccacattga gggtcaggtc tccacttgcc actttacggt
      541 aaagttttcg accgtggcgg tgaagaaagg cgcccgcgct ctcaacgaag aggagtcacg
      601 ccaggtcgcc gaggaggcgc gcgagaccga gtggacggca ccgtcgttcc tgaaagagat
      661 ctttctcggg aactaccgcc tcgacctcgt ccatccgttt cccgaggcgc cgctggatcg
      721 ccccgagttc cgggcgttca tggatcgact cgagcgcttt ctcgtcgagg aggtggactc
      781 cgaccggatc gaccgcgaag gcaaggttcc gccgcagatc gtcgagcgac tcgcggagct
      841 cggcgccttc ggaatcaaga tcccgaagga gtacggagga ctcggtttct cgcagcgcga
      901 gtacaccgag gcgatcaagc tcgtcacgag ccaggacggc aacctcacga ctctcctgtc
      961 ggcgcaccag tccatcggcc tgccgcagcc gttgaagctg ttcgggacga aggagcagaa
     1021 gaagaagtat tttccccggc tggctcgcgg agcggtgtca gcgtttgcgc tcaccgagcc
     1081 ggacgtcggc tccgatcccg cgaacctctc cacgaccgtc gagaaaacac ccgagggcga
     1141 ctacctcctg aacggcgaga agctctggtg cacgaacggc acgattgccg agctctacgt
     1201 cgtgatggcg cggcaccccg atacccggaa gatctccgcg ttcatcgtcg agcgcgattc
     1261 gccgggtgtc gaagtcgtgc accgctgcca tttcatgggg ttgaggggaa tcgagaacgg
     1321 cgtcatccgc ttcgcgaacg tgcgcgttcc gaaggaggac ctcctgttcg ccgaagggcg
     1381 cggcttgaag ctcgcgctcg tcactctgaa taccggccgg ctgacgctcc ccgcgagctg
     1441 cacggcgggc gcgaagcgag cgctcgagat ctgccgcggg tgggcgaacg agcgagtgca
     1501 gtgggggcag cccatcggtc gacacgacgc gatcgcccag cttctcgccg agatggccgc
     1561 gacgacgttc gccatggaag cgatcagcga tctcgcttcg gcgatggcgg acatgggaaa
     1621 ccgcgacatc cggctggagg cggccgtcgc caagatgtgg aactcggagg aagggtggag
     1681 gatcgtcgac cggaccatgc agatccgggg cgggcgcggc tacgagaccg cggattcgct
     1741 caaagcgcgc ggcgaagcgc cgatcgccgt cgagcggatg atgcgcgact tccgaatcaa
     1801 cctcatcttc gaaggatcgt ccgagatcat gcgcctcttc atcgcgcgcg aggcgctcga
     1861 ccggcacctc gcggtggcgc aggacctttt caacaagaag ctgacgcgcc agcagcggct
     1921 cgccacgctg ccgaagttcg tccggttcta ctcgacctgg tacccgagcc gttgggccgg
     1981 ctgggggcgg tggccccggt tcaagagctt cgggccaatg tccgggcacg tccggtacat
     2041 ggagcggacg acgcgccggc tgtcacgtgc aatctttcac ctcatggcga cgcacgggcc
     2101 gaagctcgaa aagcgccagg cgctcctttt ccgggccgtg gacatcggcg cggatctctt
     2161 cgccatgtcg gcggcggtgt cgcgggcgga cgggctgcgg cgctcgcgcg cgcccgaggc
     2221 gaagagcgcc ctcgagctgg cggacgtgtt ctgccgcatg atgcgccgcc ggatcaccgc
     2281 ggcgttcgac gcgatccgct ccaacgacga cgtacggaaa taccggatcg cgcgccgctt
     2341 cctggacggc gagcacaaat ggctcgagcg gggaatgacg ccgatggggg gcgtcgaaga
     2401 gttcgctgcg cgt
//
LOCUS       DASWAV010000126         2343 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U2665
            k141_407227, whole genome shotgun sequence.
ACCESSION   DASWAV010000126 DASWAV010000000
VERSION     DASWAV010000126.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960316
            Sequence Read Archive: SRR10260007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2343)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2343)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:47:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,929
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2343
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_407227"
                     /isolate="SMAG_U2665"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.36444 N 132.31889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..223)
                     /gene="cutA"
                     /locus_tag="VF958_02560"
     CDS             complement(<1..223)
                     /gene="cutA"
                     /locus_tag="VF958_02560"
                     /inference="COORDINATES: protein motif:HMM:NF015077.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010038 - response to metal ion [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="divalent cation tolerance protein CutA"
                     /protein_id="HEX9148022.1"
                     /translation="MRGKELIIVVTSVGTEDQALDVAHALLRNRQAACVNIIPNVHSI
                     YRWKGRVCEDDERLLIIKSVASEFEGIKET"
     gene            341..699
                     /gene="ssrA"
                     /locus_tag="VF958_02565"
     tmRNA           341..699
                     /gene="ssrA"
                     /locus_tag="VF958_02565"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            complement(881..1540)
                     /locus_tag="VF958_02570"
     CDS             complement(881..1540)
                     /locus_tag="VF958_02570"
                     /inference="COORDINATES: protein motif:HMM:NF020893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HEX9148023.1"
                     /translation="MIRQILGFLDSAPWPLVVAVVFLVSALETAIFLGLVLPGELAVM
                     VGGALASRGRVPLAGVVAAGILGPILGDSVGYFIGRRYGRRFFRGRRRERWSRARLWL
                     RRRGASAVFVGRFTAFLRSIIPAAAGAARVPLARFAPWCIAAGVIWGGGSALLGYFLA
                     RNFQALEKIASRFSLTILAVVVVGVGASILWSRRKGRRKRRHKRPRSKAAPSPRHRKS
                     A"
     gene            complement(1626..2102)
                     /locus_tag="VF958_02575"
     CDS             complement(1626..2102)
                     /locus_tag="VF958_02575"
                     /inference="COORDINATES: protein motif:HMM:NF024865.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HEX9148024.1"
                     /translation="MKILRWNPRFALLAPAIALLGAAGSAQTTSSAADAQKVAALDTQ
                     YQAAVAKNDAPTMDRILADDFVLVTGKGKVFTKTDLLNEARSGKTVYERQEEIEQKVR
                     VWGDTAVVTALLWVKGAGEDKKAFDYKLWFSDTYVRTPSGWRYVFGQASIPLPATN"
BASE COUNT          399 a          793 c          749 g          402 t
ORIGIN      
        1 tggtctcttt gatcccctcg aactcgctcg cgaccgactt gatgatcaag agccgctcgt
       61 cgtcctcgca gacgcgtccc ttccagcggt agatcgagtg gacgttcggg atgatgttga
      121 cgcaggccgc ctggcggtta cggaggagcg catgcgcgac gtcgagcgcc tggtcctcgg
      181 tgccgaccga agtgacgacg atgatcagtt ccttgccgcg catcgagggg gggatttgat
      241 tcctcggccc gggagatgtc aaggcgcccg tggaattcgc ggcggccagg gctgttaaca
      301 tcctcccgga tctttgaaga ctcgagcgaa ggttcttcgt gggggcgaaa cggcttcgac
      361 ggggacgatt gcgcgagagg ttgcatgccg aggttcactc gcctcgtaaa aaagggtgaa
      421 aaacagtagc tgcgaacaac agcaacttcg ctctggctgc ctaattaagt agccacgttt
      481 gtccggatag cgcacccgcc gcgctccggc tgacgtcgcg aaatgcgggt gtagcgactc
      541 cggacccccg gacggagtcg cgaaacgtat tcggggttgc tccgacgccg ttttgcctct
      601 tctcgaggcg gcggggcgag aaacaagaag aggctgagca tggagtggcc tctcccgtag
      661 cgcttccgga cgcgggttcg actcccgccg cctccaccat cacgtcgcgg tcctttgctc
      721 gggatccggc tccgccaccg gcgctcggct cggcggaccg gcctcgaccg agcgcctgat
      781 ccctcgccgc agtcccgctc ccgcggcgcg agcctcctcc cttcgggaga cttctcgcgc
      841 cgcgaacgga gatttaacgc gccacgaatc cactggttct tcacgcagac tttcgatgcc
      901 gtggcgacgg cgccgccttc gacctcgggc gcttgtgccg gcgctttcga cgccctttcc
      961 ggcggctcca gaggatcgaa gcacccaccc cgacgaccac gacagccagg atggtcaggc
     1021 tgaagcgcga cgcgatcttc tccaatgcct ggaagttcct cgcgaggaaa tagccgagaa
     1081 gcgcggaccc gcctccccag atcacccccg cggcgatgca ccacggcgcg aaccgcgcga
     1141 gcggaacgcg ggcggccccg gcagccgcgg gaataatcga gcgcagaaaa gcagtgaagc
     1201 gcccgacgaa cacggccgac gcgccgcgcc ggcgaagcca gagccgggcc ctcgaccacc
     1261 tctccctccg cctgccgcga aaaaaccgtc ggccgtaacg ccggccgatg aaatacccga
     1321 ccgagtcgcc gaggatcggc cccaggattc cggctgcgac cacacccgcg agcggtacgc
     1381 gtccccggga agcgagcgca cctccgacca tgacggcgag ctcgcccggg agaacgaggc
     1441 cgaggaagat cgcggtctcg agagccgaga cgaggaagac gacggcgacc acgagcggcc
     1501 agggggccga gtcgagaaac ccgaggatct gccggatcac gccgtccgcc attcaagatc
     1561 cgggcctcca acggcgggtg ggctggtctc tggcgccctc gctcggaact cccgggtgtc
     1621 cgccctcagt tcgtcgcagg cagcggaatc gaagcctgcc cgaacacgta ccgccatccc
     1681 gacggcgtcc tgacgtacgt gtcgctgaac cagagcttgt agtcgaacgc cttcttgtcc
     1741 tcgccggctc ccttcaccca cagaagcgcg gtgacgaccg ccgtgtctcc ccagacgcgg
     1801 accttctgtt cgatttcctc ctgccgctcg tagactgtct tgccgcttcg cgcctcgttc
     1861 aacagatccg tcttcgtgaa gaccttgccc ttccccgtca cgaggacgaa gtcgtcggcg
     1921 aggatgcggt ccatggtggg ggcgtcgttt ttcgcgaccg cggcctggta ctgcgtgtcg
     1981 agcgccgcca ccttctgtgc gtcggcggcg gaagaggtcg tttgcgccga gccggctgcg
     2041 ccgaggagcg cgattgccgg ggccagaaga gcgaatcgcg ggttccaccg gaggatcttc
     2101 atcgtcgtct cctgtcgagc aagtgggttc cgaatcctac cggcacttcg agtccgaccg
     2161 ctcggctaaa ctagttcggc aggctttttg atgaacacca ttcggtcgcg gacgctcgtg
     2221 gcgtgcctcg cgggatttct cgtcgtcgtc ctggcgatga tcggatccgc cgcagggcag
     2281 tcgacggcga cactcgtctt tcgcgggcag tcctatccga tcgcgacgtc cggtgcggac
     2341 ttc
//
LOCUS       DASWAV010000127         2976 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U2665
            k141_45557, whole genome shotgun sequence.
ACCESSION   DASWAV010000127 DASWAV010000000
VERSION     DASWAV010000127.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960316
            Sequence Read Archive: SRR10260007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2976)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2976)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:47:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,929
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2976
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_45557"
                     /isolate="SMAG_U2665"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.36444 N 132.31889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            146..547
                     /locus_tag="VF958_02580"
     CDS             146..547
                     /locus_tag="VF958_02580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007293543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HEX9148025.1"
                     /translation="MSALPRRPGGSRGAGWDWERVAEKALVEAGYRILDRNFRAAAGE
                     IDLVAEEGGVVCFVEVKGRRGAGFGSPAEAVTIEKQRRIFRAAQAWLKRRRRESARCR
                     FDVVSILEGSDGRRVDILRDAFEQPAARRRR"
     gene            577..1194
                     /locus_tag="VF958_02585"
     CDS             577..1194
                     /locus_tag="VF958_02585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="B-box zinc finger protein"
                     /protein_id="HEX9148026.1"
                     /translation="MPEPVLQYGYTCPSCSGSFTISLDRIPPVRARFPCAKCGEPMDF
                     PSREQAQILAKLQLEAARRETAEAELEKAPPIDRGDKVFRVDKRGWEDDFLDRRAVRN
                     LIRTAQILETDHMFTGEGKWVVAGELPDLKPLFDLKRKSKFTPPRCCRTHTDRVAHFF
                     CRDSDRPLCEECAPEKKFGANVMRVCAHCGGTISDLVEPVSEQRR"
     gene            1271..1345
                     /locus_tag="VF958_02590"
     tRNA            1271..1345
                     /locus_tag="VF958_02590"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1303..1305,aa:Gly,seq:gcc)
     gene            1548..2462
                     /locus_tag="VF958_02595"
     CDS             1548..2462
                     /locus_tag="VF958_02595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002635890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase-fold protein"
                     /protein_id="HEX9148027.1"
                     /translation="MKQRVEIVRYGFGVLFSVFAMLGCSGARVRVPASTPSPVASTRA
                     APAPDPVLPHQTFTISSRVLSETRLINLYTPPGYEKADARYPVLYMPDGGVGEDFPHV
                     TDDIDTAIRAGEMRPVIVVGIENTERRLDMTGRTTVEADRKIAPRVGGSAAFRAFIRD
                     ELMPVIGQRVRGSGQNAIVGESLAGLFIVETFFQEPRLFEIYIALSPSLWWNAQSLVR
                     GASARLKAQPRLSGTLYLASAGDDDRGDAVKALAEALRAAAPKGLTWYYEPRPDQKHS
                     TIYRAVSPGVFRKLFPPATKSAPLTPDR"
BASE COUNT          517 a          959 c          987 g          513 t
ORIGIN      
        1 tgtggctgta cgaccatccg ccgctcggcg aacgcgtcgc gttcgcgagg gggtacgacc
       61 cctgggcgaa aggcgagccg acgcgctacg tgagggagaa gatgccgtga cgccgcgggc
      121 gatcttcgag cgaatcgccg aatggatgtc ggcgctgccc cggcgccccg gcggctctcg
      181 cggtgcgggc tgggactggg agcgcgtcgc cgagaaggcg ctcgtcgaag ccggctatcg
      241 gatcctcgat cggaacttcc gcgcagctgc cggggaaatc gacctcgttg cggaggaagg
      301 cggcgtcgtc tgtttcgtcg aggtgaaggg aagaagggga gccgggttcg gatcgcctgc
      361 ggaggccgtg accatcgaga agcagcggcg cattttccgc gcggcgcagg catggctgaa
      421 gcggcgtcgg cgcgagagcg ctcgttgccg gttcgacgtc gtgtcgatcc tcgaaggatc
      481 cgacgggcgc agggtcgaca tcctgcgcga cgcgttcgag caaccggccg cgcggcggcg
      541 acggtgataa aattcgagct tccctcggga ggctaagtgc cggaaccggt tctccagtac
      601 ggctacacct gcccgagctg ctcgggcagc ttcacgattt ccctcgaccg aatccctccc
      661 gtccgcgcgc gcttcccctg cgcgaagtgc ggcgagccga tggacttccc ctcacgcgaa
      721 caggcgcaga ttctcgcgaa gctccagctc gaggcggcgc gccgggaaac ggccgaggcg
      781 gagctcgaga aagccccgcc gatcgaccgc ggcgacaagg tcttccgcgt ggacaagagg
      841 ggctgggagg acgatttcct cgaccggcgc gccgtccgca atctcattcg aactgcccag
      901 atcctcgaga ccgaccacat gttcaccggg gaggggaagt gggtcgtggc gggcgagctg
      961 ccggacctga agcccctctt cgacctgaag cgcaagtcga agttcactcc gccgcgctgc
     1021 tgccggactc acaccgaccg tgtcgcgcac tttttctgcc gcgacagcga tcggccgctc
     1081 tgcgaggagt gtgcgccgga gaagaagttc ggcgcgaacg tgatgcgcgt ttgcgcgcac
     1141 tgcggcggca cgatctccga cctggtcgag ccggtttccg agcagaggcg gtaggcgctt
     1201 ccggccgaat cgctgtcgcc cggcgcgtct tgattccccc gtcgccgttc gctatccttc
     1261 gcgtccccga gcgggaatag ctcagtggta gagcgccacc ttgccaaggt ggatgtcgcg
     1321 ggttcgaccc ccgtttcccg ctccaaccac aacacgcagg gacgcgggtt ccgccggttt
     1381 cgacccgtcg gtctcaggag cttccaaggc ccagagagtt cacgagagag ttcacggaga
     1441 gaaccgccgg cgtcgcgtac tcctgcgagg agcaaccgaa gcgctgaaca tggatccgga
     1501 ggtcgatcgc tgtgaccgcg ggcctgtggc agtggacgaa acgtgccgtg aagcagaggg
     1561 tcgaaatcgt gcgatacggg ttcggcgtgc tcttttcagt gtttgcgatg ctcggatgca
     1621 gcggcgcgcg cgtccgcgtg cccgcgtcaa caccttcacc cgtagcgagc acgcgggcag
     1681 cgcccgctcc cgatccggtt ctgccgcatc agacctttac cattagctcc agggtgctga
     1741 gtgaaacccg actcatcaac ctttacacgc cgccgggcta cgaaaaggcc gacgcccgct
     1801 atccggtgct gtacatgcca gatggtgggg ttggagaaga ttttccgcac gtcaccgacg
     1861 acatcgatac cgccattcgc gccggcgaga tgcgaccggt catcgtcgtc ggtatcgaga
     1921 acaccgagcg gcgcctcgac atgacagggc gaaccaccgt cgaagcggac aggaagatcg
     1981 cgccgcgtgt gggcggctct gctgctttcc gcgctttcat ccgcgacgag ctcatgccgg
     2041 taatcggcca gcgcgtccgc ggcagcggcc agaacgccat cgtaggcgag tcgctggcgg
     2101 ggcttttcat cgtcgaaacc ttttttcagg aaccccgatt gttcgaaatc tacatcgcgc
     2161 tcagtccgag cttgtggtgg aacgcccaat cccttgtgcg gggcgcgagc gcgcggttga
     2221 aagcacaacc gcgattaagc ggaaccctct atctggctag tgccggcgac gacgacaggg
     2281 gggacgcggt gaaggccctg gccgaggccc tgcgcgcagc tgctccgaaa ggtctgacct
     2341 ggtactacga acccaggccc gaccagaagc acagtacgat ctaccgcgct gtttcgccgg
     2401 gggtgttccg caagcttttc ccacccgcga cgaagagcgc gcctttgacg cccgaccgtt
     2461 aggccgagtg cccaagcccc gtagcagcgc tggggtacgc tcaagcgtcg tgaggatgtg
     2521 cgtcatgtcg gccggctctg tcccgtttcc cgctctcaag ccaccgttcc cacgaaggtc
     2581 caacgagagg aggctatcga cctcgctgcc gctgccacga gccgcccctg ggaataggat
     2641 tcgactagga gtcgccggtt caggcgggag cgagatcggg gcggtgacgg gcctcttcgt
     2701 tcttcagcgg gcgcacagag cagagtagtc gtggtcgtta tgggcgagga accgcagttt
     2761 cgtcgagcgc cagcgcccga atggcaactt tccggaatct ggacttccat tcacacgaca
     2821 gctcgcaaat cgccgtcgaa tgcctgaccg gagaagttcg ctcaggctcc tcctcgtaac
     2881 cgccttcatg gccctttccg gttgccgcac gctggaccga gcgctgcaac ataccgggat
     2941 cccgtgtggc gcctatcgtt ggccggtgaa gactct
//
LOCUS       DASWAV010000128         2005 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U2665
            k141_6917, whole genome shotgun sequence.
ACCESSION   DASWAV010000128 DASWAV010000000
VERSION     DASWAV010000128.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960316
            Sequence Read Archive: SRR10260007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2005)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2005)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:47:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,929
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2005
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6917"
                     /isolate="SMAG_U2665"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.36444 N 132.31889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..471)
                     /gene="pgsA"
                     /locus_tag="VF958_02600"
     CDS             complement(<1..471)
                     /gene="pgsA"
                     /locus_tag="VF958_02600"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011687959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008444 -
                     CDP-diacylglycerol-glycerol-3-phosphate
                     3-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HEX9148028.1"
                     /translation="MKINLPNALTLLRIFLVPFLLVVLLTKFEGRETVGLIIFLFATA
                     TDFFDGWLARRRGEITTLGTLLDPIADKLLISAAFVSLVELGLAPAWMVVVILGREFA
                     VSGLRSIASGQGIVIAASGWGKAKMFSQIVAASLLILATRHDVFLLPGRIALWFV"
     gene            577..1986
                     /locus_tag="VF958_02605"
     CDS             577..1986
                     /locus_tag="VF958_02605"
                     /inference="COORDINATES: protein motif:HMM:NF014877.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucomutase/phosphomannomutase family
                     protein"
                     /protein_id="HEX9148029.1"
                     /translation="MSIPFGTDGWRGVIADDCTFDAVRRVAAATARVYVERNGPGDRS
                     RIVVGHDTRFFSPEFARAAAEVFARAGIDVLLTDRPLPTPAVSFHVRRLGLAGGVAIT
                     ASHNPAPYNGFKVKAHFGGSAPPELYAEISESADRPLPEAARPGRIDTVDLLGPYREG
                     LASLLDLGAIRSAGLGVLADSLHGAAGTLIADIAAGGATRIISFRAERDALFGGVHPE
                     PIAPNLEASSREVVAQKLDLVVAHDGDADRLGVLDRRGRFVSPHRVLALLLLHGFRRR
                     ALSGGIAKTFSTSLLIDRVAQKLGVPLFETAIGFKHVASLMTSGEAAAGGEESGGYAF
                     AFHLPERDGVLNALLLLESLSQSGRDLDGALADLEAEFGAFAYGRRDVYLPVPVIAAY
                     LRDVASSPPRAVGGVPVTGVARKDGVKYLFGGSGWLLHRLSGTEPMVRLYCEHGEEET
                     VERVLEEAEGRLREFARAN"
BASE COUNT          323 a          689 c          681 g          312 t
ORIGIN      
        1 gacgaaccag agcgcgatcc tgccgggcag gagaaaaacg tcatggcgcg tcgcgaggat
       61 gagaagcgac gccgccacga tctggctgaa catcttcgcc ttgccccagc cggacgcggc
      121 gatcacgatc ccctggccgg aagcgatcga gcgcaatccc gagacggcga actcgcgtcc
      181 gaggatcacg accaccatcc acgccggggc gaggccgagc tcgacgagcg agacgaacgc
      241 cgccgaaatc agcagcttgt cggcgatcgg atcgagaagc gtgccgagcg tcgtgatctc
      301 cccgcgtcgc ctcgcgagcc agccatcgaa gaaatcggtc gcggtcgcga acagaaagat
      361 gatgaggccg acggtctccc gcccctcgaa cttcgtgagc agcacgacga gaaggaaggg
      421 gacgagaaag atgcgcagga gggtcagcgc gttcgggagg ttgatcttca atcgcttttt
      481 ccagtccgtt cgacttggta cggtatcttc cctcgcgggg cgaagagccg tgtttaaggt
      541 aacaccggcc gcggacgagc cgcgggaccc gcgttcttga gcatcccgtt cggcacggac
      601 gggtggcgcg gcgtcatcgc cgacgactgc actttcgacg cggtccggcg cgtggcggcg
      661 gcaaccgcga gggtctacgt cgagcgaaac ggccccggcg accggagtcg gatcgtcgtc
      721 ggccacgaca cccgcttctt ctcacccgaa tttgcccgag cggccgcgga ggtttttgcg
      781 cgcgccggga tcgacgttct tctgaccgat cggccgcttc cgacgcccgc cgtctccttc
      841 cacgtccggc ggctcggcct cgccggcggg gtcgcgatca ctgcgagcca caatcccgcg
      901 ccctacaacg gattcaaggt gaaggcccac tttggcggca gcgctccgcc cgagctgtat
      961 gccgagatct cggagagcgc cgaccggccc ctgcccgagg cggcccgacc cgggaggatc
     1021 gacacggtcg acctgctggg tccctaccgc gaaggactcg cctcgctgct cgacctcggc
     1081 gcaatccgct ccgcgggcct cggcgtgctg gccgattcgc tccacggggc cgccggaacg
     1141 ctcatcgcgg atatcgcagc cggaggggcg acacggatca tctctttccg ggcggagcgc
     1201 gacgccctct tcggcggggt ccacccggag ccgatcgcgc ccaacctcga agcctcgtcg
     1261 cgcgaagtgg tcgcgcagaa gctcgacctc gttgtcgcgc acgacggcga cgcggaccgc
     1321 ctcggtgtgc tcgatcggcg cgggaggttc gtgtcccccc accgagtgct ggcgctcctc
     1381 cttctccacg gattccgccg ccgcgcgttg agcggaggga tcgcgaagac attctccact
     1441 tctctgctca tcgaccgggt ggcgcagaag ctcggcgttc cccttttcga gaccgcgatc
     1501 ggtttcaagc acgtcgccag cctgatgacc agcggcgagg ccgcggccgg cggcgaggag
     1561 agcggaggct acgcgttcgc atttcacctt cccgaacggg acggcgttct caacgcgctc
     1621 cttctgctcg agagcctgtc ccagtcggga cgcgatctcg acggcgcgct cgccgacctc
     1681 gaggcggagt tcggggcttt cgcctacggc cgccgcgacg tctacctgcc ggtgccggtg
     1741 atcgcggcct atctcaggga cgtcgcaagc tcccctccgc gggccgtggg cggagtcccg
     1801 gtcaccggcg tagcgcggaa ggacggggtc aagtacctct tcggcggcag cggctggctg
     1861 cttcaccgac tctcgggcac ggagccgatg gtgcgcctct attgcgagca cggggaggag
     1921 gaaaccgtcg agcgggtgct cgaggaagcg gagggccgcc tgcgcgaatt cgcgagggct
     1981 aactgattcg acgcagcccc ggcgt
//
LOCUS       DASWAV010000129         3013 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U2665
            k141_326275, whole genome shotgun sequence.
ACCESSION   DASWAV010000129 DASWAV010000000
VERSION     DASWAV010000129.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960316
            Sequence Read Archive: SRR10260007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 3013)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3013)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:47:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,929
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3013
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_326275"
                     /isolate="SMAG_U2665"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.36444 N 132.31889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(42..1100)
                     /locus_tag="VF958_02610"
     CDS             complement(42..1100)
                     /locus_tag="VF958_02610"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEX9148030.1"
                     /translation="MKAPRVLVAAVQSPFSGGGAERHVQRLTQELEKRGVEAELVTMP
                     LIERDRFDLVRSALGWRSLDVTEFGNRGVDAVIATRFPSYVVRHPNKIVWVIHQYRQA
                     YDQFGTPWSDFTASAEDRRTRDTIAAIDRLGLSEARRVFANSKTVAARLARYNGIASE
                     PLYHPPPLVGRYRTGPFGDYALTVGRLDGWKRADLPLRALAYAPAARLAVVGRGPEEE
                     RLKRLARDLGVDGRVRWIPRADEEELLDLYSGARIVVVAPQGEDLGYVPLEAFLSGKP
                     VLTTDDAGGPLEFVVDGETGFVVPPRPDALGAALAKAWDREDALAALGRRGLERASRL
                     SWDETIGRLMAAAGLAVP"
     gene            complement(1126..2256)
                     /locus_tag="VF958_02615"
     CDS             complement(1126..2256)
                     /locus_tag="VF958_02615"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX9148031.1"
                     /translation="MATADELAAELAREIRAAVAETGPSEDIAPDERESEEFSDIRLP
                     LHRAELYLTPTVPDASRLAGPKSAALRLLRFLWRGQASFNALALEAANGIVGALERTR
                     AAIERQTRRFDDFTRELARLEKEARRCDSAQDTRIAYLEALSSRSSPSSSLPRTETPL
                     LPESVYALFEERFRGSSIEVAEKQRFYLPFLWNLPGPVLDAGCGRGEFLRLLKDEGIV
                     GRGVEASGLAAEACRALGLDVREGDAIEALAAAPPGSLGAVVAFQVVEHWPAAATFRF
                     LAEARRALAPGGVLIVETVNTNSLAAMNAFYLDPTHVRPVPPEALRFLCDAAGFRELR
                     VEFLSPVPAGERLEERSENDARLNAMLFGPQDYAVIGWVPQA"
     gene            2360..2734
                     /locus_tag="VF958_02620"
     CDS             2360..2734
                     /locus_tag="VF958_02620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9148032.1"
                     /translation="MIRGFILLAAAAITAACASSGPPASPALLAEAEAAVRRSEAAGA
                     GERAPDLLAKARRAWDEGRLASTRGEGTTATRRLEEAREYARAAEAQANAERTRSEAA
                     TLKQQADELEARTRQIRSAPTP"
     gene            2748..>3013
                     /locus_tag="VF958_02625"
     CDS             2748..>3013
                     /locus_tag="VF958_02625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9148033.1"
                     /translation="MRRSLTIVSITLTLFVAGCSSGPPPREIAEARLAVQDAKNASAD
                     QLATRDYEAALVNLRMAENTWDARKDSAGAAHWARMAEATARLA"
BASE COUNT          490 a         1063 c         1053 g          407 t
ORIGIN      
        1 ggattcgcgc gcgagacgtc ggagggattc cgtatcggcg atcacgggac cgccagcccg
       61 gccgccgcca tcaggcggcc gatcgtttcg tcccaggaga gccgcgacgc tctctcgagg
      121 cctcgccgcc ccagcgcggc gagcgcgtcc tcccggtccc aggccttcgc gagcgccgcg
      181 ccgagcgcat cggggcgcgg cggtacgacg aatcccgtct cgccgtcgac gacgaactcg
      241 agcggcccgc cggcgtcgtc cgtcgtcagg accggctttc cggagagaaa agcctcgagc
      301 ggcacgtatc ccagatcttc gccctggggc gcgacgacga cgatccgcgc gcccgaatag
      361 aggtcgagga gctcctcctc gtccgcgcgc gggatccagc gcacccggcc gtcgacgccg
      421 aggtcgcggg cgagcctctt caacctttcc tcctccgggc cccggccgac gacggcgagc
      481 cgcgccgcgg gcgcgtacgc cagcgctcga agcggcaggt ccgcgcgctt ccagccgtcg
      541 aggcgcccca cggtcagcgc gtagtctccg aacggtccgg tgcggtagcg cccgacgagc
      601 ggcggcgggt ggtagagcgg ctcgctcgcg atcccgttgt acctggcgag ccgcgccgcc
      661 accgtcttcg agttcgcgaa gacccggcgc gcttccgaga ggcccagccg gtcgattgct
      721 gcaatcgtgt cgcgcgtccg gcggtcttcg gccgaggctg tgaaatcgct ccacggggta
      781 ccgaactggt cataggcctg ccggtactgg tgaatcaccc agacgatctt gttcggatgc
      841 cgcaccacgt agctcgggaa gcgcgttgcg atgacggcgt ccaccccgcg gttcccgaat
      901 tccgtcacgt cgagggaacg ccagccgagc gcggagcgca ccaggtcgaa gcggtcgcgc
      961 tcgatcaagg gcatcgtcac gagttccgcc tcgacgccgc gcttctcgag ctcctgggtc
     1021 agacgctgga cgtgccgctc cgctcccccg ccggagaacg gtgactgcac cgccgcgacg
     1081 agaacccgcg gcgccttcac gttcgattcc gcgggaggag ggcgctcacg cctgagggac
     1141 ccaaccgatc acggcgtaat cctgcgggcc gaagagcatc gcgttcaagc gagcgtcgtt
     1201 ctccgagcgc tcctcgagtc gctcgccggc cggtactgga gagaggaatt cgacgcgaag
     1261 ttcccggaac ccggccgcat cgcagagaaa gcggagcgct tcgggcggca ccgggcgcac
     1321 gtgcgtggga tcgagataga acgcgttcat cgccgcgagt gaattcgtgt tgacggtctc
     1381 gacgatcagg acgccgccgg gcgccagcgc tcgacgcgcc tcggcgagga accggaacgt
     1441 ggcagcggcc ggccagtgct cgacgacctg gaacgcgacg acggccccga ggctcccggg
     1501 cggcgccgcc gccagcgctt cgatggcgtc cccctcccgc acgtcgagcc cgagagcgcg
     1561 gcacgcctcg gcggcgagac cgctcgcctc cacgcctcgc ccgacgatgc cctcgtcctt
     1621 caggaggcga aggaattcgc cacggccgca acccgcatcg agaacgggac ccggcaggtt
     1681 ccacagaaac gggagataga agcgctgctt ctcggcgacc tcgatcgagg agccgcgaaa
     1741 tctttcctcg aagagcgcgt acacgctctc gggaaggagc ggagtctccg ttcggggaag
     1801 gctcgacgac ggcgacgagc gggaggaaag cgcctccagg taggcgatcc gggtatcctg
     1861 tgcggagtcg cagcggcgag cctccttctc gagacgcgcg agctcccgtg tgaaatcgtc
     1921 gaaccttctc gtctgccgtt cgatcgccgc ccgcgttctc tcgagtgccc cgacaatgcc
     1981 gttcgcggcc tcgagcgcga gcgcgttgaa cgaggcttgc ccccgccaga gaaaacgcag
     2041 gaggcgaagc gcggcggact tgggccctgc gagccggctc gcgtcgggga ccgtcggcgt
     2101 gagatagagc tcggcgcggt ggagaggaag acggatgtcg gaaaactcct ccgactcccg
     2161 ctcgtcggga gcgatgtcct ccgacgggcc cgtctccgct acggccgcgc ggatctcccg
     2221 cgcgagctca gccgcgagtt cgtctgccgt cgccatggat gtcacgcgat tatccccaat
     2281 gccgcgccag tcgctggcag cgtaaatgca gtaaagtcgg cgagagtcat tacctcgctc
     2341 caccgagagg aggtccaaga tgatcagagg tttcatcctg ctggcagccg cggcgatcac
     2401 ggccgcctgt gcgtcgagcg ggccgcccgc gtccccggct ctcctcgccg aggcggaagc
     2461 tgcggtccgg cgttccgaag cggccggggc cggcgagcgg gcgcccgatc tgctcgcgaa
     2521 ggcgcggcgc gcctgggacg aggggcgcct cgcgtccacc cggggagaag gaaccacggc
     2581 gacgcgccgc ctcgaggaag cccgcgaata cgcccgcgcc gcggaggcgc aggcgaacgc
     2641 cgagcggacg cggagcgagg ctgccactct caaacagcaa gccgacgagc tcgaggcgag
     2701 gacgcgacag atccggtccg cgccgactcc gtgaaggagg tagtccgatg cgcagaagcc
     2761 tgacgattgt ttcgatcaca ctcaccctgt tcgtcgcggg ctgttcgtcc gggcccccgc
     2821 cgcgcgaaat cgcggaggcg cggctggccg tccaggacgc gaagaacgcg agcgccgacc
     2881 agctcgcaac gcgcgactac gaggccgcac tcgtcaacct gcggatggcg gagaacacgt
     2941 gggacgcccg gaaggactca gccggcgccg cgcactgggc gaggatggcg gaggccactg
     3001 cacggctcgc gca
//
LOCUS       DASWAV010000130         4725 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U2665
            k141_367724, whole genome shotgun sequence.
ACCESSION   DASWAV010000130 DASWAV010000000
VERSION     DASWAV010000130.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960316
            Sequence Read Archive: SRR10260007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 4725)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4725)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:47:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,929
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4725
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_367724"
                     /isolate="SMAG_U2665"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.36444 N 132.31889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1325)
                     /gene="murJ"
                     /locus_tag="VF958_02630"
     CDS             complement(<1..1325)
                     /gene="murJ"
                     /locus_tag="VF958_02630"
                     /inference="COORDINATES: protein motif:HMM:TIGR01695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0034204 - lipid translocation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HEX9148034.1"
                     /translation="MSESAPPTEEARGRLVRSASVITLLTLASRLTGYARDRVIAALF
                     GASAASDVFYTAFRIPNLLRQLVAEGALPGVFIPSYAEKRKDGSAEDARVFAARVLSA
                     LTVIVTAITLLGIVFAPPIVRLLAKGFASTPGKIPLTIFLTRLMFPYLCFISVAALLQ
                     AILNAHGRFAVSAMSPILLNLSIILSAVVLAPRFREPTVALAVGVLLGGVLQMAIQIG
                     PARRLGAIGTWSIAFSDPAVRAVFVLLLPRIFGFGIYAINNALSTRFASVFGDGAVSY
                     LTYGNRVIELVRGGFVISVSTAILPLLAQQALEDDSRAFKDTLRFGLRLVAFVTIPWA
                     VGLAVLRNTVIAALFQGGRFGPAATAATAQALALYAVGLFFISSSSLLVFAHYARKDT
                     RTPVACGVIDLAIFIAVALLLRPWGVGSVALATSCGAIANFVLLLASLRR"
     gene            1438..1857
                     /locus_tag="VF958_02635"
     CDS             1438..1857
                     /locus_tag="VF958_02635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013149210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HEX9148035.1"
                     /translation="MASVNKVILIGNLGRDPEIRTTPQGTSLARFSVATSTTWKDASG
                     AKQERTEWHDVVAWEKLAQICGEYLHKGKMVYVEGSLQTRSWEDQNGQKRYKTEIKAS
                     NVVMLSPPRDRSETARPAKEESEAAEPVAAYDDDVPF"
     gene            complement(1900..2934)
                     /locus_tag="VF958_02640"
     CDS             complement(1900..2934)
                     /locus_tag="VF958_02640"
                     /inference="COORDINATES: protein motif:HMM:NF013126.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 N-terminal
                     domain-containing protein"
                     /protein_id="HEX9148036.1"
                     /translation="MSTRASRLFGVGIPGTSIESKERAILERYPPRAVILFRRNIEEE
                     RQLEDLTADIRSLPGEPFLCLDQEGGPVDRLRSVVGPFPSFHAAARAGFARRAGELAG
                     EACARFGFDVDLAPVVDRRLPGAGETVLGERAAAEDPDVVAGAARDFLRGLHSHGIGG
                     CLKHFPGLGRARSDTHKSLPLLSSDAAEREKDLAPFRALQEIAGAIMVSHAAGEDGVP
                     ATLSRDVATTLLREGVGFRGAAFTDDLEMGALDAFGGLAARAAAAVRAGCDLPWVCSR
                     IEEYPECVEQVERDVAEDRLAEASARIEAYVERLADLRRRAVTPSRPLEVLAAHIAAL
                     RETIAESIQT"
     gene            complement(2931..>4725)
                     /gene="mutS"
                     /locus_tag="VF958_02645"
     CDS             complement(2931..>4725)
                     /gene="mutS"
                     /locus_tag="VF958_02645"
                     /inference="COORDINATES: protein motif:HMM:NF003810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HEX9148037.1"
                     /translation="SAVATLELFEASDGDRRRSLCALVDRTRTPMGARSLKDAISRPA
                     LDPIELTERWDAVEELVAKPDARERLRRELEGIGDLERQFGRIAVGTAGPRDVLAWSA
                     GLRRLPAVRTAGEALVSPRLRLLLDGIPDVGDLADRVERTIAPDAPALASSGGVLRAG
                     ADAELDSLRELRRGAQGALLAIEAEERRRSGISNLRVRFNRVFGYSLEVGAAHRERVP
                     ADWIRRQSLANAERFVTPALKELEAKILGAEERIGEIESRMYGELLAGLTGGADRLSR
                     AAAAAGAIDLLASFAEVAHSGRWVRPRLSPTPRIAIVDGRHPLVEHHRREEPFVPNDC
                     DLSPEKRILVITGPNMGGKSTYLRQVGTIILLAQAGSFVPATAAEISPVDRIFTRVGA
                     ADHLSRGESTFMVEMLEAAAIVRDATPSSLILLDEVGRGTSTFDGLSIAWALVEHLHD
                     SPEKSGFVLFATHYPELTEIALVKPAVSNATMAVKEVGGRVVFLKKVVPGAADRSYGI
                     HVAELAGLPASVTARAREVLSNLEKQELDLQGAPVIARRGGESAGEGQLLLFSGSEEL
                     VLEKLREADVDRMTPIAALSFLASLRERLKG"
BASE COUNT          833 a         1586 c         1689 g          617 t
ORIGIN      
        1 cgccggagag atgccaggag caggacgaag ttggcgatcg cgccgcagga cgtcgcgagc
       61 gccacggagc cgactcccca gggccgcaga agaagcgcca cggcgatgaa tatggcgagg
      121 tcgatgacgc cgcacgcaac cggcgtgcgc gtgtccttgc gggcgtagtg cgcgaacacg
      181 agcaggctgc tggaggagat gaagaagagc ccgacggcgt agagcgcgag cgcctgcgcg
      241 gtcgccgcgg tcgcggccgg gccgaagcgc ccgccctgga agagcgcggc gatcacggtg
      301 ttccgcaaga cggccagccc gacggcccac gggatcgtca cgaaggcgac gaggcgcagc
      361 ccgaaccgga gcgtgtcctt gaacgctcgg gagtcgtctt cgagcgcctg ctgggcgagg
      421 aggggcagga tcgccgtcga aaccgagatc acgaatccgc cgcgcacgag ctcgatcacc
      481 cggttgccat aggtcaggta cgacaccgct ccgtctccga agaccgaagc gaaacgcgtc
      541 gagagggcgt tgttgatcgc atagatgccg aagccgaaga tccgcggcag gagcagcacg
      601 aagacggcgc ggaccgccgg gtcggaaaac gcgatgctcc acgtcccgat cgcgccgaga
      661 cgacgggccg gcccgatctg gatcgccatc tggagcactc ccccgaggag aactccgacc
      721 gcgagcgcca ctgtcggctc gcgaaatcgc ggggcgagca cgaccgcgga gaggatgatc
      781 gaaaggttca ggaggatcgg cgacatcgcc gagaccgcga accggccgtg cgcgttcagg
      841 atcgcctgga ggagcgcggc cacggagatg aagcagaggt agggaaacat gaggcgcgtc
      901 aggaagatcg tcagcgggat cttccccggg gtcgaggcga accctttcgc gagcagccgc
      961 acgatcgggg gcgcgaagac gattccgagg agagtgatcg ccgtgacgat gacggtcagg
     1021 gcggagagaa cccgggccgc gaagacgcgt gcgtcttccg ccgatccgtc cttgcgcttc
     1081 tcggcgtacg aagggatgaa aacgcccggc aatgctccct ccgcgacgag ctgccggagc
     1141 aggttcggaa tgcggaaggc cgtgtagaag acgtcgctcg cagcgctcgc cccgaacagc
     1201 gcagcgatga cccggtcgcg ggcgtaaccg gtgagccggg acgcgagcgt gaggagagtg
     1261 atgaccgagg cggatcggac cagacgcccg cgcgcttcct ccgtcggcgg cgccgactcg
     1321 ctcaagccga gagaaccatc gtgcgcctca ttgtaaaagc cacgcgaaag ccccggacat
     1381 catttgtccc gaccgcgcgc gatatccttc ggagcacttg aaaggagagc ggaacacatg
     1441 gccagcgtca acaaagtcat tctgatcggc aacctcgggc gggacccgga gatccggacg
     1501 acgccgcagg ggacgtcgct cgcgcgcttc tcggtcgcga cttccacgac gtggaaggac
     1561 gcgtccggcg cgaagcagga gcgcacggag tggcacgacg tcgtcgcctg ggagaaactc
     1621 gcccagatct gcggcgagta cctccacaag ggaaagatgg tgtacgtcga gggaagcctc
     1681 cagacccgga gctgggaaga ccagaacggt cagaagcgat acaagacgga gatcaaggcg
     1741 tcgaacgtcg tgatgctctc gcctccgcgg gatcggtccg agacggcccg gccggccaag
     1801 gaggagagcg aagcggccga gccggtcgcg gcatacgacg acgacgtgcc cttctgagaa
     1861 tgtttgcggg gacaggcact acgggcctgc acccgcatcc tatgtctgta tcgactccgc
     1921 gatcgtttcg cgcagtgcgg cgatgtgcgc cgcgagcact tccagagggc gcgaaggcgt
     1981 gaccgcccgg cgtcgcagat ccgcgagccg ctcgacgtag gcctcgatcc tcgcagacgc
     2041 ctcggcgagc cggtcctcgg ccacgtctcg ctcgacctgc tcgacgcatt cggggtactc
     2101 ctcgatgcgt gagcacaccc acggcaggtc gcacccggcg cggacggccg cggcggcgcg
     2161 cgcggcgaga ccgccgaacg cgtcgagcgc gcccatctcg aggtcgtcgg tgaaggcggc
     2221 gccgcggaac ccgacgcctt cgcgcaggag ggttgtcgcg acatcccggg aaagggtcgc
     2281 cggtaccccg tcctcgccgg ccgcgtgcga gaccatgatc gctcccgcga tttcctggag
     2341 cgcccggaag ggagcgaggt ctttctcgcg ctcggccgcg tcgctactga ggagaggaag
     2401 agacttgtgg gtgtcgcttc gggctctccc gagccccgga aagtgcttca ggcagcctcc
     2461 gatgccgtgc gagtgcagac cgcgcaggaa gtcacgcgcc gcgccagcca ccacgtcggg
     2521 atcttcggcg gcggcgcgct ccccgaggac cgtctctccc gcgcccggca ggcggcggtc
     2581 gacgaccggc gcgaggtcca cgtcgaagcc gaagcgcgcg cacgcctctc ccgcgagctc
     2641 accggcgcgg cgcgcgaaac cggcgcgggc ggcggcgtga aaggaaggaa acggcccgac
     2701 gacgctccgg agccgatcca cggggccgcc ctcctgatcg aggcagagga acggctcgcc
     2761 cgggagcgat cggatgtccg ccgtgagatc ctcgagctgc cgctcctcct cgatgttgcg
     2821 ccggaagagg atgacggcgc gcggcggata ccgctcgagg atcgcacgct ccttcgactc
     2881 gatcgaggtc cccggtatcc cgacgccgaa gaggcgcgac gcgcgggtgc tcaccccttc
     2941 agccgctccc ggagagacgc gagaaacgag agcgcggcga tcggagtcat gcggtcgaca
     3001 tccgcctcgc gcagtttctc caggacgagc tcctccgaac cgctgaagag gaggagctgg
     3061 ccttccccgg cgctctcgcc gccgcgacgc gcgatcaccg gcgctccctg caggtcgagc
     3121 tcctgcttct cgaggttcga caggacctcg cgcgcgcggg ccgtgacgga cgcgggaagc
     3181 ccggcgagct cggcgacgtg gatcccgtag ctgcgatcgg cggcgccggg cacgaccttc
     3241 ttcaggaaga ccacccggcc tcccacctcc ttcaccgcca tcgtggcgtt ggagacggcg
     3301 ggcttgacga gcgcgatctc cgtcagctcg gggtagtggg tcgcgaagag tacgaagccg
     3361 ctcttctccg gtgagtcgtg gaggtgctcg accagcgccc acgcgatcga caggccgtcg
     3421 aacgtggacg tcccgcggcc aacctcgtcc aggagaatca ggctcgacgg cgtggcgtcg
     3481 cggacgattg ccgccgcctc gagcatctcc accatgaatg tggactcgcc gcgcgaaagg
     3541 tgatccgccg cgccgacccg cgtgaagatg cgatcaacgg gtgagatctc cgccgccgtg
     3601 gccgggacga acgaccccgc ctgcgcgaga agaatgatcg ttccgacctg ccggaggtag
     3661 gtggacttcc ctcccatgtt gggcccggtg atgaccagga tccgcttctc cggagacagg
     3721 tcacagtcgt tcggcacgaa cggctcttcg cgccggtggt gctcgacgag cggatggcgg
     3781 ccgtccacga ttgcgatccg gggcgtcgga gagagccgcg ggcgaaccca gcgcccggag
     3841 tgggcgactt ccgcgaacga ggcgaggaga tcgatcgctc cggcggccgc ggcggcgcga
     3901 gagaggcggt ccgctcctcc cgtgagacct gcgaggagtt cgccatacat cctcgactcg
     3961 atctcgccga tgcgttcctc ggcgccgagg atcttcgcct cgagctcctt cagcgcgggg
     4021 gtgacgaagc gctcggcgtt cgcaagcgac tgccgccgga tccagtcggc gggcacacgc
     4081 tcgcggtgag cggctccgac ctcgagcgag tacccgaaga cccggttgaa acgcacgcga
     4141 aggttggaga tcccggagcg ccggcgctcc tccgcttcga tcgcgaggag cgccccctgc
     4201 gcgccgcggc ggagctcacg gagcgagtcg agttcggcgt cggcgccggc gcgcagcacg
     4261 cctcccgagg aggcgagcgc cggggcgtcg ggggcgatcg tgcgctcgac ccgatccgcg
     4321 aggtctccga cgtcgggtat cccgtcgagc agaaggcgaa ggcgaggcga aacgagcgcc
     4381 tccccggccg tacgaacggc cggcagccgc cgaagaccgg cgctccaagc gaggacgtcg
     4441 cgcggacctg cggtcccgac cgcaatacga ccgaactgac gctcgaggtc cccgatgccc
     4501 tcgagctcgc gccggagtct ctctcgcgcg tccggcttcg cgacgagctc ctcgactgcg
     4561 tcccaccgct cggtgagttc gatcggatcg agagcgggcc gcgagatcgc gtccttgagg
     4621 gagcgggccc ccatcggggt gcgcgtacgg tcgacgagcg cgcaaaggct gcggcggcgg
     4681 tcgccgtcgg aggcctcgaa gagctcgagc gttgccaccg ccgac
//
LOCUS       DASWAV010000131         2419 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U2665
            k141_407622, whole genome shotgun sequence.
ACCESSION   DASWAV010000131 DASWAV010000000
VERSION     DASWAV010000131.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960316
            Sequence Read Archive: SRR10260007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2419)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2419)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:47:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,929
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2419
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_407622"
                     /isolate="SMAG_U2665"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.36444 N 132.31889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1089
                     /gene="pheS"
                     /locus_tag="VF958_02650"
     CDS             <1..1089
                     /gene="pheS"
                     /locus_tag="VF958_02650"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006930875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HEX9148038.1"
                     /translation="PDSSAHGSKVTDRDDDPIEQVLVAFRAETAGGVSSRAEFEALKG
                     RYLGREKGLVPALFARMREIPAPDRAAYGTRANAAKREVEEGIARLGESLAHEEARRR
                     ELAAAVDVTLPGRRIRAGSLHPVTVVRRRIEEIFLRMGYSIADGPEVESDLHNFEALN
                     FPPEHPARDAQDTLFLDREAPSALLLRTHTSPVQIREMRKRGAPLKIIAPGRVYRNES
                     VDATHQPVFHQVEALVIDHGISMADLKGTVETFFEALFGVGTRVRIIPTFFPFVEPGA
                     DVHVSCIFCRGVGCRTCKGSGWMELAGAGEVHPKVLEACGIDPEEWSGFAFGLGLDRV
                     AMMLYEFPDLRLLYEGDERFLSQFSPLS"
     gene            1086..>2419
                     /locus_tag="VF958_02655"
     CDS             1086..>2419
                     /locus_tag="VF958_02655"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: protein motif:HMM:NF015448.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HEX9148039.1"
                     /translation="MKFSLEWLGDRVSVADAGGADGARRLLDRAGLPVESVEESGTGA
                     VFDVEITPNRPDAMSHLGLAREIAAMAGIPFRGEIATGAVLLREDGPPASELASVEIE
                     VPRLCRRFGARVIRGTKSVPSPEGVLSRLRAIGAKPIDAAVDATNYVLWDTGQPLHAF
                     DLDRLAGGRIVVRKAKRGEKLVTLDGVERTLEPADVVVADAERAVSLAGIMGGLDTAV
                     TAATTNVLLEAAWWDPVAIRRTSRRLGMHTDASHRFERGADPEAIPGALDLAARLLVE
                     AAGGTLAPGRIDARGTAWPRRRVVLRLDRLRLIGGDPRLDLEFAADALARLGFRVATR
                     SAKRLTAEAPSWRPDISIEEDLVEEVLRVWGYDRIPSRLPATAGAGGHLEPLRLTEER
                     LADLAVASGLHETYSYPFVDRRTDEASFAPWLESTGTAASPLSVANPVDASRR"
BASE COUNT          336 a          849 c          861 g          373 t
ORIGIN      
        1 cccgactctt cggctcacgg ctcaaaggtc acggatcgag acgacgatcc gatcgagcag
       61 gtcctggtcg ctttccgggc tgaaaccgcg gggggcgtgt cgtctcgcgc cgagttcgag
      121 gcgctcaagg ggcgctacct cggccgcgag aaggggctcg tccccgctct cttcgcgcgg
      181 atgcgggaga ttcccgcacc ggaccgcgcc gcctatggca cgcgcgcgaa cgccgccaag
      241 cgggaggtcg aggaggggat cgcccgcctg ggtgaatcgc tcgctcacga ggaggcccgg
      301 cggcgcgagc tcgcggccgc cgtcgacgtc acgctccccg gccggcggat ccgcgcggga
      361 tcgctccatc cggtcaccgt ggtccgccgc cgcatcgagg aaatcttcct gaggatgggc
      421 tactcgatcg cggacggacc ggaggtcgag agcgacctcc acaacttcga ggcgttgaac
      481 ttcccgccgg agcatcccgc ccgcgatgcc caggacactc ttttcctgga ccgggaggcg
      541 ccctcggcgc ttctccttcg cacccacacg tctccggtgc agatccggga gatgaggaag
      601 cgcggcgcgc cgctcaagat catcgctccg gggcgcgtct accgaaatga atcggtggat
      661 gcgacgcacc agccggtgtt ccaccaggtc gaagcactcg tgatcgacca cgggatctcg
      721 atggcggacc tgaagggcac cgtcgagacc ttcttcgagg ctctgttcgg cgtcggcacg
      781 agggtccgga tcatcccgac cttcttcccc ttcgtcgagc cgggcgccga cgttcacgtg
      841 agctgcatct tctgccgcgg cgtgggctgc cgcacgtgca agggctccgg ctggatggag
      901 ctcgcgggag cgggggaggt gcatccgaag gtcctcgaag catgcgggat cgatccggag
      961 gagtggagcg gcttcgcgtt cggactcggc ctcgaccgcg tcgcgatgat gctctacgag
     1021 tttcccgatc tgcgacttct ctacgagggg gacgagcgct tcctctcgca gttttcgccg
     1081 ctctcgtgaa gttctcgctc gaatggctgg gagaccgcgt ctccgtcgcc gatgcggggg
     1141 gcgcagacgg cgcgcgtcgc ctgctcgacc gcgccggact tcccgtcgaa tcggtcgagg
     1201 agtcggggac tggcgccgtc ttcgacgtcg agatcactcc aaaccggccg gacgccatga
     1261 gccacctcgg cctcgcccgc gagatcgcgg cgatggcggg gattccgttc cggggggaaa
     1321 tcgcgaccgg cgccgtcctg ctgcgtgagg acgggccgcc agcgtcggag ctcgcctccg
     1381 tcgagatcga ggtcccgcgc ctctgccggc gcttcggcgc gcgggtcatc cgcggcacga
     1441 agagcgttcc gtcgcccgag ggcgtcctct cccgccttcg ggcgatcggc gccaagccga
     1501 tcgacgcggc cgtcgacgcg acgaactacg tcctctggga taccggccag ccgctgcacg
     1561 cgttcgacct cgaccggctc gccggcggac ggatcgtcgt gcgcaaggca aagcggggtg
     1621 aaaagctcgt cactctggat ggcgtcgagc gcacgctcga gccggcggac gtcgtcgtcg
     1681 ccgacgcgga gcgggcggtc tcgctcgccg ggatcatggg cggcctcgat acggccgtca
     1741 ccgcagcgac gacgaacgtg ctcctcgaag cggcgtggtg ggacccggtc gcgatccgcc
     1801 ggacttctcg ccggctcgga atgcacacgg acgcgtctca ccgcttcgag cgcggcgccg
     1861 acccggaggc gatccccggc gcgctcgatc tcgccgcgcg gcttctcgtc gaagccgccg
     1921 gaggcacgct cgcgccgggg cggatcgacg cccgggggac tgcgtggccg cgccgccgcg
     1981 tcgtcctgcg gctcgaccgg ttgaggctga tcggcggcga cccgcgcctc gacctggaat
     2041 tcgcggcgga cgcgctcgcg cgactcgggt ttcgggtggc aacgaggagc gcgaagcgcc
     2101 tgacggccga ggcgccctcg tggcgcccgg acatctcgat cgaagaggac ctcgtcgaag
     2161 aggtgctccg agtctggggt tacgaccgga ttccctcccg cctgccggcg accgccggcg
     2221 cgggcgggca tctcgagccg ctgcgcttga ccgaggagag gctcgccgac cttgcggtcg
     2281 cctccgggct gcacgagacg tactcgtatc cgttcgtcga ccggcgaacc gacgaggcgt
     2341 ccttcgcgcc ctggctcgaa tcgaccggca ccgcggcgag cccgctgtcg gtggcgaacc
     2401 cggtcgacgc gtcgcggcg
//
LOCUS       DASWAV010000132         6834 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U2665
            k141_326654, whole genome shotgun sequence.
ACCESSION   DASWAV010000132 DASWAV010000000
VERSION     DASWAV010000132.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960316
            Sequence Read Archive: SRR10260007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 6834)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6834)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:47:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,929
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6834
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_326654"
                     /isolate="SMAG_U2665"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.36444 N 132.31889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..534)
                     /locus_tag="VF958_02660"
     CDS             complement(<1..534)
                     /locus_tag="VF958_02660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015716872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylation factor-like protein"
                     /protein_id="HEX9148040.1"
                     /translation="MVFFNASTRELTAKIVYYGPGLGGKTTNLKVLHQRLEPGTAGDL
                     VALSTESDRTIYFDLLPVELGDIKGYRIRFQLTTVPGQTAFNETRRVVLKGADGVVFV
                     ADSQWTLLPKNLESWQSLKENLAANGLALGTTPVVIQYNKRDLPDILSVEAMQEALGF
                     SAYPHVEAIASEGRGVTE"
     gene            695..1255
                     /gene="pyrR"
                     /locus_tag="VF958_02665"
     CDS             695..1255
                     /gene="pyrR"
                     /locus_tag="VF958_02665"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013495313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HEX9148041.1"
                     /translation="MTEALLLKKAKILDAERIGRMIDRMAHEIAERQKDLPGLALVGI
                     RTRGVPLAMRLAERLSDLVGNPFPVGTLDITLYRDDLSTVAPQPLLKKTEIEFDLNGR
                     AVILCDDVLYTGRTIRAALDGVIDLGRPRVIRLAVLIDRGHREFPIEANYVGKSVPTA
                     QREVIKVMFQETDGADEVWILERPGS"
     gene            1313..2266
                     /locus_tag="VF958_02670"
     CDS             1313..2266
                     /locus_tag="VF958_02670"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013822381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0016597 - amino acid binding [Evidence
                     IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HEX9148042.1"
                     /translation="MNRPIAALRSKDLLGIEPLEADEIVLLLDTADSMRTIAERPIKK
                     VPALRGRMIVNLFFEASTRTRFSFETAEKWLSADSLNFSGGKGTSVEKGETLLDTART
                     LQAMSPDLVVVRHSSAGVPHLLARHLEAGVINAGDGAHEHPTQALLDALTIRQRLGRL
                     RGLRVVIAGDIAYSRVARSNVFLLTKMGADVVLCGPPTLLPPHLEDLGVAVTTDLDRA
                     IEGANVVMVLRMQLERQRRSLFPSTREYFTRYGLTAERLSRAHEDAIVMHPGPMNRGV
                     EIASDVADSARSVILDQVANGVAVRMAVLYLLLARRPWEGM"
     gene            2263..3549
                     /locus_tag="VF958_02675"
     CDS             2263..3549
                     /locus_tag="VF958_02675"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012633496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004151 - dihydroorotase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HEX9148043.1"
                     /translation="MTDLLVRGGRIVDPSVGRDGAADVLLAGGAVAAVGEGLSAEGAE
                     VLDAAGLYVFPGFIDLHAHLREPGREYAETIASGAAAAAAGGFTAVCAMPNTEPTNDD
                     RSVTSFIAARGAESGAARVYPIGAITRGRKGNELAEFGEMRRAGAVAVSDDGEWLADG
                     DLLRRAIQHASLYGLPVVQHAEDPTLSRRAPMHEGAVSTRLGLAGQPDAAEAAAVARD
                     ILVARLAGGRLHFAHLSTALSVGFLREAKSRGLPITGEATPHHLTLTDVEVARSGYST
                     RFKMNPPLRERGDVDALVEALEDGTIDAIATDHAPHHEDEKAVDFDTAPFGVVGLETA
                     VSVVHDRLVRTGRLSLRRFVELFSTGPARVFGLPGGTLAPGSPADITLFDPRKRWTVD
                     PTRFLSLARSTPFAGWDLVGAPAATIVAGRLVRTGR"
     gene            3559..5757
                     /locus_tag="VF958_02680"
     CDS             3559..5757
                     /locus_tag="VF958_02680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9148044.1"
                     /translation="MVVSLCLVAFLGFLLSDAPPVASPAAIRHVAIVLPRGWPAERFE
                     RWSAFALREGIEMRLVGEGEPEAAGWEAVRFAAPPVSESFRRRLAGFPVTLEATGFVF
                     DGRPYRGSGDAVVLTHPDRPGETLVLGNSRDAAIRIARWIFRDSPVAPGRFRALSGEL
                     TKEGRFRRGRGGLEIDRASERDRIAERERFFGSLQTKRRGVAVWRFQESERRAYERWA
                     PVLDRLLLPAKSRLAVTVFLYPDPSRKGLFMGSSRPADLSWGKDGARVDVDASVPAEP
                     DLVTPVLASAAIGARQPRLRDRPALLLAAGARVRGRWWGRDVASFAAFLERAQVQPRV
                     EDVLSAEEREDVSPICIVGAAAAWLDAGVREGGAAALERAFAEDEGELAATLARWRTA
                     AQRVPVFAPRRRPLPAGFLRGVSYAMSNSIEDSYASPRSLETLKRLSTMSVNSIAVIP
                     YGYSRTEHMPELAFVHRSPRGETDEGSVRALTDARSLGMTAMLKPQLWIGGDAFVGTV
                     AMGSPDDWAKWFRAYRRFAVHHAIVAEASGAALFCVGTELSGTEPREREWRDTIAAVR
                     LATGAALVYTTNWASGAPRVRFWDALDAIGVDFYDPLSADRAASDAALAAGARRAAEP
                     VARLSAATGKPVLFAEAGYPPAPAAWTAPHDDGSAGPLAPADAARAIRAVFAGLGREK
                     WWRGVYWWKAFSDGRDADASDRGFNFLGRPAGDAIAAAFRSLAAAEGDSR"
     gene            5754..6560
                     /locus_tag="VF958_02685"
     CDS             5754..6560
                     /locus_tag="VF958_02685"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007162415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008934 - inositol monophosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006020 - inositol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0046855 - obsolete inositol phosphate
                     dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HEX9148045.1"
                     /translation="MSGSSLVEVAADAARAGGDLIERYWRRLPGDSVSEKRANDFVTR
                     ADKESEEVIVARIRDRYPEDGFLGEEGGRRGAGGERRTWIIDPLDGTSNFIAGFPFWS
                     VSVAARERGEIVAGAIWDPLRRELYSAERGSGAFRDGERLKVTARENLDGAFLATGFP
                     FRAKDRIDLYLSIFRELFLHARAIRRAGSAALDLAQVAAGVFDGFFEFKLSVWDIAAG
                     ALLIEEAGGRICDFDGGSGYWERGNVVAGAPGVAGGIVRVASRLFSDSEI"
     gene            complement(6606..>6834)
                     /locus_tag="VF958_02690"
     CDS             complement(6606..>6834)
                     /locus_tag="VF958_02690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007850667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEX9148046.1"
                     /translation="ALLAEAEEELRYRRVTVSSIRRAAHPDDAIFAEIGAGADLVAMG
                     AHGHGRVVELVLGSTTDRVLRAASVPVLLAG"
BASE COUNT          961 a         2346 c         2424 g         1103 t
ORIGIN      
        1 ctcggtcacc ccccgacctt cgctcgcaat cgcctcgacg tgcggatagg ccgaaaatcc
       61 gagggcctcc tgcatggctt cgaccgaaag gatgtcgggc aggtcgcgct tgttgtactg
      121 gatcacgacc ggcgtcgtgc cgagcgcgag cccgttcgcc gcgaggtttt ccttgagcga
      181 ctgccagctc tcgagattct tcggcaggag cgtccactgg gaatccgcga cgaagacgac
      241 cccgtcggcg cccttcagga cgacgcggcg ggtctcgttg aacgccgtct gtccgggcac
      301 cgtcgtgagc tgaaagcgga tccgatatcc cttgatgtcg ccgagttcca cggggagaag
      361 gtcgaagtaa atcgtgcggt cgctctcggt cgaaagcgcg acgagatcgc ccgcggtccc
      421 gggctcgagc cgctggtgga ggaccttcag gttcgtcgtc tttccgccga gccccgggcc
      481 gtagtagacg atcttcgccg tcagctcgcg cgtcgacgcg ttgaagaaga ccatgggagt
      541 tcctcgccgc tttacgaggg attgtaacgg actcggtcgc ggtcggggtc cgcggcgttc
      601 gcttcgagaa cggtggccgg aaatttcgga cgagagtatc ccgctccgcc ttgcagttgc
      661 ttcctcaccg acaagccact agactcgcgc ggacttgacc gaagccctcc ttctcaagaa
      721 ggcgaagatc ctcgacgcgg agcgcatcgg ccgcatgatc gaccgcatgg cgcacgagat
      781 cgccgagcgc cagaaggacc ttcccggcct cgccctcgtc gggatccgga cgcgcggcgt
      841 ccccctcgcg atgcgtctcg cggagcggct ctcggacctc gtcgggaatc cctttcccgt
      901 tgggacgctc gacatcacgc tttatcgcga cgacctctcg acggtcgcgc cgcaaccact
      961 cctgaagaag accgagatcg agttcgacct gaacggccgc gccgtgattc tctgcgatga
     1021 cgtgctctac acggggcgca cgattcgcgc ggcgctggac ggtgtgatcg acctcggccg
     1081 gccccgggtg atccggctcg ccgtactgat cgaccgcggg caccgcgaat ttccgatcga
     1141 ggccaactac gtgggcaaga gcgttccgac cgcgcagcgc gaggtcatca aagtgatgtt
     1201 ccaggagacc gacggcgccg acgaggtctg gatactggag aggcccggct cgtagggacg
     1261 ggaatcggaa atcggtattc gggaattcgg ggagccagga ggtcgcgaca ccgtgaaccg
     1321 accgatcgcg gctctgcgct ccaaggacct cctcggcatc gagccgctcg aggcagacga
     1381 gatcgtcctc ctcctcgaca ccgcggattc gatgcggacg atcgcggaac gcccgatcaa
     1441 gaaggttccc gctctgcgcg ggcgcatgat agtgaacctc ttcttcgagg cttcgacccg
     1501 gacacggttc tcgttcgaga ccgccgagaa gtggctttcg gcggactcgc tcaacttcag
     1561 cggcggcaag gggacctcgg tcgagaaggg cgagacgctg ctcgataccg cgcgaacgct
     1621 ccaggcgatg tcgcccgacc tggtcgtcgt gcggcattcc tcggcggggg ttccgcatct
     1681 cctcgcgcgg cacctggagg cgggcgtgat caacgccggc gacggtgccc acgagcaccc
     1741 gacgcaggcg cttctcgacg cgctcaccat ccggcagcgc ctgggccgcc tgcgcggcct
     1801 ccgtgtcgtg atcgccggcg acatcgcgta ctcccgcgtg gcgcgttcga acgtcttcct
     1861 gctgacgaag atgggcgcgg acgtggtcct ctgcggcccg ccgactctcc tccctccgca
     1921 tctggaagac ctcggcgtcg ccgtgacgac ggacctcgat cgcgcaatcg agggcgcgaa
     1981 cgtggtcatg gtgctgcgca tgcagctcga gcggcagcgg cgctctctct tcccctcgac
     2041 ccgtgagtac ttcacgcggt acgggctcac cgccgagagg ctgagccgcg cgcacgaaga
     2101 cgcgatcgtc atgcatcccg gacccatgaa ccgcggggtc gagatcgcat cggacgtggc
     2161 cgattcggcg agatcggtga tcctcgacca ggtggccaac ggcgtggccg ttcggatggc
     2221 cgttctgtac ctgctgctgg cccgccggcc gtgggagggg atgtgacaga tctcctcgtc
     2281 cggggagggc ggatcgtgga cccctctgtc gggcgggacg gcgccgccga tgtgcttctc
     2341 gcgggcggag cggtcgccgc cgtgggggaa gggctctccg cggagggcgc cgaggtcctc
     2401 gacgcggcgg ggctctacgt ttttcccggc ttcatcgacc ttcacgcgca cctgcgcgag
     2461 ccgggccgtg aatacgcgga gacgatcgcg tcgggggccg ccgcggcggc tgccggaggt
     2521 ttcaccgcgg tctgcgcgat gcccaacacg gagccgacga acgacgaccg cagcgtgacg
     2581 tcgttcatcg ccgcgcgcgg cgcggagagc ggcgcggcgc gcgtctatcc gatcggagcc
     2641 atcacgcgcg gccgaaaggg aaacgagctc gccgagttcg gcgagatgag gcgcgccggg
     2701 gcggtcgcgg tgtcggacga cggcgagtgg ctggcggacg gcgatctgct gcgccgggcg
     2761 atccagcatg cgtccctcta cgggctgccg gtcgtacagc acgcggagga cccgacgctc
     2821 tcgcgccgcg cgccgatgca cgagggagcg gtgtcgacgc ggctcggcct tgccggccag
     2881 cccgacgcgg ccgaggcggc ggcggtggct cgagacattc tcgtcgcgcg cctggccggg
     2941 gggcgcctgc actttgcgca cctctcgacg gcgctgtcgg tcggattcct gcgcgaggcg
     3001 aagtcccgcg gcctgccgat caccggcgag gcgactccgc accatctgac gctcaccgac
     3061 gtggaggtgg cacgctcggg atactccacg aggttcaaga tgaacccgcc gctgcgcgag
     3121 cgtggcgacg tcgacgcgct cgtcgaggcg ctggaggacg gcacgatcga cgcgatcgcg
     3181 accgatcatg ctccgcacca cgaagacgag aaggcggtcg atttcgatac cgcgccgttc
     3241 ggggtggtgg gtctcgaaac cgccgtttcg gtcgtccacg accggctcgt tcgtaccgga
     3301 cgcctttcgc tccgccgctt cgtcgagctc ttctcgaccg ggcccgcgcg ggtgttcggg
     3361 ctgccggggg gaaccctcgc gccaggctca cccgccgaca tcacgctctt cgatccccgc
     3421 aagcgctgga cggtggatcc cacgcgcttc ctgtcgctcg cgcgctcgac gccgttcgcc
     3481 ggttgggatc tcgtcggcgc cccggccgcg accatcgtcg ccggccgctt ggtccgcacc
     3541 gggcgctgag tcagtcgcgt ggtcgtctcc ctctgcctcg tggcgttcct cggtttcctt
     3601 ttgtcggacg cgccccccgt cgcctcgccc gctgccatcc gccacgtcgc gatcgttctg
     3661 ccccggggat ggccggccga gaggttcgag cgctggagcg cgttcgcgct ccgcgaaggg
     3721 atcgaaatgc ggctcgtcgg cgaaggcgaa cccgaagccg ccgggtggga ggcggtgcga
     3781 ttcgcggcac cccccgtttc cgaatccttt cgccggcgcc tcgccgggtt cccggtgacg
     3841 ctcgaagcga cgggcttcgt cttcgacggc cggccgtatc gcggttcggg cgacgccgtc
     3901 gtcctcactc atcccgaccg ccccggcgag acgctcgtgc tcggaaactc gcgagacgcc
     3961 gcaatccgga tcgcccgctg gatcttccgg gactctccgg tcgcgcctgg ccgcttccgc
     4021 gcgctctccg gcgagctgac aaaggagggt cgttttcggc gcggacgggg cggcctggag
     4081 atcgaccggg cgagcgagcg agaccggatc gccgagcgcg aacgattctt cggttctctc
     4141 caaacgaaga ggcgcggcgt cgccgtgtgg cggtttcaag agagcgagcg ccgcgcttac
     4201 gagcgctggg cgcccgtcct cgatcgactt ctcttgccgg cgaagtctcg ccttgccgtc
     4261 acggtgttcc tctatcccga cccttcccgg aaagggctct tcatgggctc gtcccgtccc
     4321 gccgacctct cgtgggggaa ggacggcgcg cgggtcgacg tcgacgcgtc cgtgccggcg
     4381 gagcccgacc tggttacccc cgtcctcgcg agtgcggcga tcggcgcgcg gcagccacga
     4441 ttgcgcgacc ggccggccct cctcctcgct gcgggcgccc gcgtacgggg acgctggtgg
     4501 gggagggacg tggcttcgtt cgccgcgttc ctcgagcgcg cgcaggtaca gccgagggtg
     4561 gaagacgtgc tctccgccga ggagagagag gacgtctcgc cgatctgtat cgtcggagcg
     4621 gcagccgcgt ggctcgacgc cggcgtgcgc gaggggggag ccgccgctct cgagcgagca
     4681 tttgccgaag acgagggcga gctcgcggcg acgctcgcgc gctggcgaac agcagcgcaa
     4741 cgggtcccgg tcttcgctcc gcgccgccgg ccgctgccgg cgggcttcct gcggggcgtg
     4801 tcttatgcca tgtcgaactc gatcgaggac tcctacgcct cgccgcggtc cctcgagacc
     4861 ttgaagcgcc tgtcgacgat gtccgtcaac tcgatcgccg tcatccccta cgggtactcg
     4921 cgcacggagc acatgccgga gctcgcgttc gtccatcgca gcccgcgcgg agaaaccgac
     4981 gaggggagcg ttcgcgcgct gaccgacgct cgatcgttgg gcatgacggc gatgctgaaa
     5041 ccgcagctct ggatcggcgg ggatgcgttc gtcgggacag tcgcgatggg aagcccggat
     5101 gactgggcga agtggtttcg ggcgtatcgg cggttcgccg tgcaccacgc gatcgtcgcg
     5161 gaggcgtcgg gagcggcgct cttctgcgtc ggcacggagc tgtcgggaac cgagccccgg
     5221 gagagagagt ggcgagacac gatcgccgcc gtgcggctcg ccacgggagc agcgctcgtc
     5281 tacacgacga actgggcgtc cggcgcgccg cgcgttcggt tctgggacgc actcgacgcc
     5341 atcggcgtcg acttctacga cccgctgtcg gcggatcggg ccgcgtcgga cgccgcactc
     5401 gccgccggtg cgcgccgcgc agcggagccg gtggcgaggc tatcggcggc gacggggaaa
     5461 ccggtcctct tcgccgaggc cggttacccg ccggccccgg cggcgtggac ggcgccgcac
     5521 gacgacgggt ccgcgggccc tctcgcgccc gcagacgctg cgcgcgccat ccgcgccgtc
     5581 ttcgcggggc tcggacgcga gaagtggtgg cggggggtct actggtggaa ggcgttttcg
     5641 gacggccggg acgcggacgc ctccgaccgc ggcttcaact tcctcgggcg gcccgcggga
     5701 gacgccatcg cggcggcctt tcgaagcctt gccgccgccg aaggcgactc gcggtgagcg
     5761 gaagctcact cgtcgaggtc gccgccgatg ccgcccgcgc cggcggcgac ttgatcgagc
     5821 gctactggcg aaggctcccc ggcgactccg tctccgagaa gcgtgcgaac gacttcgtca
     5881 cccgcgcgga caaagagagc gaggaggtta tcgtcgcgag gatccgtgac cgctacccgg
     5941 aggacggctt cctcggggag gaggggggcc ggcggggcgc cggcggcgag cggcgcacct
     6001 ggatcatcga tccgctcgac ggcacgtcca acttcatcgc gggctttccg ttctggtccg
     6061 tctcggtcgc ggcgcgcgag cgcggagaga tcgtcgccgg cgcgatctgg gacccgctcc
     6121 gccgcgagct ctactcggcc gagcgcggca gcggcgcctt ccgcgatggc gagcgcctga
     6181 aggtgacggc ccgggagaac ctcgacggcg cctttctcgc gaccggattt cctttccgcg
     6241 cgaaggaccg catcgacctc tacctgtcga ttttccgtga gctctttctc catgcccgag
     6301 cgatccggcg cgccggctcc gcggctctcg acctcgcgca ggtggcggcc ggcgtcttcg
     6361 acgggttctt cgagttcaag ctctcagtct gggacatcgc tgccggcgcg ctcttgatcg
     6421 aggaagcggg cggacgcatc tgcgacttcg acggcggttc gggatactgg gagcgcggaa
     6481 acgtcgtggc gggcgcgccc ggcgtcgctg ggggaatcgt gagggtcgcg tcccggctgt
     6541 tctccgattc cgagatctga tcggccgcgg gcgctttcaa gcggcgccga agggttccga
     6601 cgatttcagc cggcgagaag aacggggacg ctcgctgcgc gcagcacgcg gtcggtcgtc
     6661 gaaccgagga cgagctcgac cacgcggccg tgaccgtggg cacccatcgc gacgaggtcc
     6721 gcgcccgcgc cgatctccgc gaagatcgcg tcgtcggggt gagccgcgcg acggatgctc
     6781 gacaccgtga cgcgccggta ccggagctcc tcctcggctt ctgcgagaag ggcg
//
LOCUS       DASWAV010000133         3336 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U2665
            k141_407812, whole genome shotgun sequence.
ACCESSION   DASWAV010000133 DASWAV010000000
VERSION     DASWAV010000133.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960316
            Sequence Read Archive: SRR10260007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 3336)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3336)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:47:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,929
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3336
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_407812"
                     /isolate="SMAG_U2665"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.36444 N 132.31889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2473
                     /gene="alaS"
                     /locus_tag="VF958_02695"
     CDS             <1..2473
                     /gene="alaS"
                     /locus_tag="VF958_02695"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HEX9148047.1"
                     /translation="DLDNVGYTARHHTFFEMLGNFSFGDYFKDDAIRFAWELLTDRQI
                     GYGLEPDRMWATVYLSDDDAAALWERYLPKERILRFGEAENFWAMGDTGPCGPCSEVH
                     YFRGNDLSNNLPGLVNGEGDETVEVWNLVFMQYERDTAGKLDPLPRPSVDTGAGLERL
                     TCVLGGASSNYDTDLFAGLIEALSRISGRSYRGGMAVEDAPFRVIADHARAVTMLMAD
                     GVLPSNEGRGYVLKRIVRRAMRYARQLGIEGPVLHRLVAEVVGSFTGAYFTPAEAAVA
                     IERVVPPLSAEEERFAKTLSAGETLVAEEIERLRRSGERTLPGEVLFRLYDTHGIPVD
                     VIEEIARDEGLQVDRPGFEAAMGAQRARSRATAKFEAADVSVYGALDLPEVHSQFRGY
                     PEQDFVHLEGAKAVGLIGPRGAVPRLLSGETGEAVADRTVFYPEGGGQVADTGRWTWP
                     GGEAEVVDVRKPVPNLISHRVRVVRGELAVGTVVTMDVPEWVRRRTQANHTGTHLLHA
                     ALRKVLGAGARQMGSLVAPDRLRFDYASNVAPTNDQIAEIERLVNEEVLRDREVTKSI
                     LPMEEAKRRGADMFFWDKYSDRVRVVEVPGFSTELCGGCHVPRTGEIGAFKIVSDKSL
                     AAGVRRLEAVTSLGAVELLRHDEETLIRLSAAAQSSRDVLAERWIEREERMKALEREV
                     ASLRMKLASGESGGGEAETEEIGGIRIVTRLVRGLSIPELRNLSDTLRSRIKSGVVVV
                     GSSNDGKTSVVTAVTPDLVDRVPASRIASRVGKALGGSGGGKPDLAQAGGKSEELLPA
                     GLAAAKEALRELLSAEGEGTASAAR"
     gene            2527..3222
                     /locus_tag="VF958_02700"
     CDS             2527..3222
                     /locus_tag="VF958_02700"
                     /EC_number="4.2.2.n1"
                     /inference="COORDINATES: protein motif:HMM:NF013618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HEX9148048.1"
                     /translation="MRSKTLIALGILFALAAPGRAKVRVVVQNGKKVIFNDGVGEKRP
                     AGTKRSNDQWLATRISRPSPYDTLISRAAADHSVDPRLVKSVMLVESGFNASAVSRKG
                     ARGLMQLMPGTATKHGVKDAHNPQQNIAGGTKYLSHLLDLFDGNLEKSLAAYNAGEAA
                     VARFGGIPPYDETRDYVRKALTAYYGMPYLGGVSGALSGGFGKPVRVERDSKNRPVLT
                     TGHPATPGLRRIS"
BASE COUNT          544 a         1097 c         1171 g          524 t
ORIGIN      
        1 cgacctcgac aacgtcggct ataccgcgcg ccaccatacg ttcttcgaga tgctcgggaa
       61 cttctcgttc ggcgactact tcaaggacga cgcgatccgc ttcgcctggg agctcctgac
      121 cgaccggcag atcggttatg gcctcgaacc ggatcggatg tgggcgaccg tgtatctctc
      181 cgacgacgac gcggcggcgc tctgggagcg ctatctgccg aaggaaagga tcctgcggtt
      241 cggggaggcg gagaacttct gggcgatggg ggacacgggg ccctgcggac cgtgctcgga
      301 agtgcactac ttccggggga acgacctctc gaataacctc ccggggctcg tcaacggcga
      361 gggggacgag acggtcgaag tctggaatct ggtcttcatg cagtacgagc gcgacaccgc
      421 agggaagctc gatccgcttc cgcgtccgtc ggtggacacc ggcgccggcc tcgagcggtt
      481 gacgtgcgtg ctgggcggcg cttccagcaa ctacgacacg gatctgttcg cgggactgat
      541 cgaagcgctc tcgcgcatct cgggacgttc ctatcgcggc ggcatggcgg tcgaggacgc
      601 gccgttccgc gtgatcgccg accatgcccg cgcggtcacg atgctgatgg ccgacggggt
      661 ccttccttcc aacgaagggc gcggctacgt cctgaagagg atcgtgcggc gcgcgatgcg
      721 atatgcgcgt cagctcggaa tcgaagggcc ggtcctgcac cggctcgttg ccgaagtcgt
      781 gggctcgttc accggcgctt acttcacgcc cgccgaggcg gcggtcgcga tcgagcgggt
      841 cgtccctccg ctctccgcgg aggaggagcg ttttgcgaag acgctgtctg ccggcgaaac
      901 gctcgtcgcc gaggagatcg agcgcctccg gcgctccgga gagcgcacgc tcccgggcga
      961 ggtgctcttc cggctctacg acacgcacgg cattcccgtc gacgtgatcg aggagatcgc
     1021 gcgcgacgaa gggctccagg tggaccgccc gggcttcgag gcggcgatgg gagctcagcg
     1081 cgcgcgctcc cgggcgaccg cgaagttcga ggcggcggac gtctccgtct acggcgccct
     1141 cgacctgccg gaagtccact ctcagttccg cggatacccg gagcaggatt tcgtgcacct
     1201 cgagggcgca aaggccgtcg ggctcatcgg tccccgcgga gccgtgcccc ggctcctgtc
     1261 gggggagacg ggagaggcgg tcgccgaccg cacggtcttc tacccggagg ggggcggaca
     1321 ggtcgccgat accggccggt ggacctggcc gggaggagag gcggaggtcg tggacgtccg
     1381 caagccggtg ccgaacctga tctcgcaccg cgtacgcgtc gtccgcgggg agctcgcggt
     1441 cgggacggtg gtgacgatgg acgttcccga gtgggtccgc cgccggacgc aggcgaatca
     1501 cacgggcacc cacctgttgc acgcggccct tcgaaaggtg ctcggcgccg gcgcccggca
     1561 gatgggctcc ctcgtcgcgc ccgaccgctt gcgcttcgac tacgcgtcca acgttgcgcc
     1621 gacgaacgat cagatcgccg agatcgagcg cctcgtcaac gaggaggtcc tgcgcgaccg
     1681 cgaagtgacg aagtcgatcc tccccatgga ggaggcgaag cgtcgcggtg cggacatgtt
     1741 cttctgggac aagtactccg atcgcgtgcg cgtcgtcgag gtgcccggat tctcgacgga
     1801 gctgtgcggc ggctgccacg tgccgcgcac cggagagatc ggagcattca agatcgtctc
     1861 ggacaagtcg ctcgccgccg gagtgagacg actcgaggcg gtcacgtcgc tcggcgccgt
     1921 cgagctgctg cggcacgacg aagagacgtt gatccggctc tccgcggcgg cgcagtcctc
     1981 gcgcgacgtg cttgccgagc gctggatcga gcgcgaggaa cggatgaagg cgctcgagcg
     2041 ggaggtcgct tcgctgcgga tgaagctcgc gtcgggcgag tcgggcggcg gggaagcgga
     2101 gaccgaggag atcgggggga tccgcatcgt gacgaggctc gtccgcgggc tctcgattcc
     2161 cgagttgcga aacctctccg acactctgcg ctcccggatc aagagcgggg tcgtcgtcgt
     2221 cgggtcgtcc aacgacggga agacgtcggt tgtgacggcc gtcacgccgg atctcgtcga
     2281 ccgcgtgccg gcttcccgga ttgcctctcg cgtcgggaag gcgctcggag gcagcggggg
     2341 cgggaagccg gacctcgccc aggcgggcgg aaagagcgaa gaacttctgc cggcagggct
     2401 tgccgccgcg aaggaggcct tgcgcgaact gctttcggcc gaaggtgagg gaacggcctc
     2461 cgccgcccgc tgattccggg actataattc gcgtatcgtt cgttcgagac cacgacccac
     2521 gaattcatgc gctcgaagac cctgatcgcc ctgggaattc tgttcgccct cgccgcgccc
     2581 gggcgcgcga aggtccgggt cgtcgtgcag aacgggaaga aagtcatctt caacgacggg
     2641 gtcggcgaga agcgaccggc aggaacgaaa cgctcgaacg accagtggct cgcgacgcgg
     2701 atctcccgcc cttcaccgta cgacaccctg atcagccgcg cagccgccga ccactccgtc
     2761 gatccacgac tcgtcaagtc cgtcatgctg gtcgaatcgg gattcaacgc gtccgcggtc
     2821 tcccggaagg gcgcgcgcgg cctgatgcaa ctcatgcccg ggaccgcgac caaacacgga
     2881 gtgaaggacg cccacaaccc gcagcagaac atcgccggcg ggacgaaata cctttcgcac
     2941 ctgctcgacc tcttcgacgg aaacctcgag aagtcgctcg cggcttacaa cgcgggggaa
     3001 gcggcggtcg cgcgcttcgg cgggattccg ccctacgacg agacgcgtga ttacgtgcgc
     3061 aaggccctga cggcttacta cgggatgccg tacctgggcg gcgtctccgg cgctctcagc
     3121 gggggcttcg gcaagcccgt gcgtgtcgag cgtgactcga agaaccgccc ggttctcacg
     3181 acggggcacc cggcaacgcc ggggctgcgt cgaatcagtt agccctcgcg aattcgcgca
     3241 ggcggccctc cgcttcctcg agcacccgct cgacggtttc ctcctccccg tgctcgcaat
     3301 agaggcgcac catcggctcc gtgcccgaga gtcggt
//
LOCUS       DASWAV010000134         5268 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U2665
            k141_407836, whole genome shotgun sequence.
ACCESSION   DASWAV010000134 DASWAV010000000
VERSION     DASWAV010000134.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960316
            Sequence Read Archive: SRR10260007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 5268)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5268)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:47:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,929
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5268
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_407836"
                     /isolate="SMAG_U2665"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.36444 N 132.31889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            34..1863
                     /locus_tag="VF958_02705"
     CDS             34..1863
                     /locus_tag="VF958_02705"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012375851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HEX9148049.1"
                     /translation="MAAVVIAAAVLPAATNKPPLHARKWLAITGKPLAATAGAMIFQK
                     GGNAVDAACAMIAATCTMWDTLSWGGETQALIYNPHTGKVVGVNALGVAPTGATPEFF
                     HSKGMAYPPEYGPLAAVTPGTPGGIMVMLAEYGKLSLAEVLAPAIEMADGYPIEQQLV
                     STVTRQRDWLLKWPESRRVLLSHPGDRPLAAGEVFRQPDLAATLRKLVEAEKQALAAG
                     KSRRDAIFAANDRFYKGDIAREFVRGSQEIGGLHTVEDLSNWKVRIEEPVRTTYKGID
                     VYKLDFWTQGPAMLQALNILETADVKAMGYNSARYLHALYQAMNLAFADRDFYYGDTY
                     FPPEEPRRGLLSKEYAKARAAKIDWTKNDPNVKPGDPYRFQGGKNPYVDLLEKWPPPA
                     AKAKAESAGPDAANRVPDFREAFYSGTTSVEAADAEGWVVSVTPSGGWVPAAIAGKTG
                     IGMSQRMQSFVLDRAESPFNVLEPGKHPRVTLTPTLALKDGKPYLCFSVQGGDSQDQN
                     LLQFFLNVVEFGMTVQEATEAANINSYQMRSSFGAHEARPGAMMIHPLLPPWVRSELA
                     RMGYKLEVAPKTSGPINAIFLDREHGTMWGGSSDHGEDYGIGW"
     gene            1880..3337
                     /locus_tag="VF958_02710"
     CDS             1880..3337
                     /locus_tag="VF958_02710"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014425.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HEX9148050.1"
                     /translation="MKRDAIRWIAFFAAVKLLFHFLSNGLYGFHRDELATLDDARHLA
                     WGYVAYPPLTPFLGQIELALFGATPPAFRLLAALAQSIAIVLTAMTAKCLGGDRSAQW
                     IAAIAVAISPISLAASALFQYVAFDFLWWVLIAYFAVRLIESDDPRWWIAIGTVIGLG
                     ALTKYTIAFYVAGIVAGVLATDLRRHLRSRWLWIGMVVSIAIALPHFLWEANHGFVTL
                     EFLRHVHARDVRIGRTKDFVLDQIRVASNPVTIPLSIVGLIAVLQSRRFRMLAWMVLV
                     PFALFLLAQGRGYYTGPLYPILLAAGSVITEQLVKSRRRAAFAVMAVLAAIGCAVVPI
                     AIPLARPRTRLFRIASALNHDLREEFGWPEMAREVDRIGASLSPEERAHSAALCANYG
                     EAGAISLYGRTPVLSGVNSFWARGYGNPPPRTLIVLGFGREGLERRFAEVVPAGRIPN
                     PYGLENEESERPEIYVCRGLREPWPEFWRRLRSFG"
     gene            complement(3345..3770)
                     /locus_tag="VF958_02715"
     CDS             complement(3345..3770)
                     /locus_tag="VF958_02715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9148051.1"
                     /translation="MRKANHLARSGWFWTLVLASALVLPAMELGAQTVQPTPTPTPDL
                     KTKVKVVEVTKTGTALSVKPDKVQIKMNQDIVVWVTNGVSMKITFKPGNPSPGNPFTD
                     LVCKGRFCAALTPSAAQGTFAYKVTVDGVVLDPNVEVIP"
     gene            complement(3815..>5268)
                     /locus_tag="VF958_02720"
     CDS             complement(3815..>5268)
                     /locus_tag="VF958_02720"
                     /inference="COORDINATES: protein motif:HMM:NF025910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX9148052.1"
                     /translation="TDEFSAEEKDFVRVARSTGASLILSGAIQRTGDQLRITCSLVRA
                     PDGVHIGGDEVTTSASEIFGAQDRLAESVFSWLGAKRRAESPARPGTVPLSSAAQETY
                     LKAIGALRRYDVPESIATAIRILTDLDRKHPDSPLILAALGRAHLNQFEITRDPASAE
                     RAIAACEAARKLDDRLPDVHTTLGQVLRMTGKPADAAREFERVLAEQPSSIDALLGLA
                     DAYRSTRDPRAEATYRRALALQPSYWAVYNQFGFFYYQGGRYSEAIPMFREAINRRPD
                     SVRAYNNLGAALFKADRFAEAREAYQGSIRINPSDGAYTNLGNLEYYVGNYRAAAAAF
                     EEATKLTPGKYLYWANLGDAYRWTPELESKAPAAYEKAVQLAERELSLNPNNAAAHAT
                     VAICDAKLGRKDPARLHIRRALEIEPANPDHFLYAAIVADLAGKSDEAIGWLGKAVKA
                     GLGAAQIEREPELKNLRSLPGFGEALASAKSRT"
BASE COUNT          812 a         1746 c         1773 g          937 t
ORIGIN      
        1 tggggagccc ggacctggcg cggaatcctc ggcgtggcgg cggtagtgat tgccgctgcg
       61 gtgctgccgg cggcgaccaa caagccgccg ctccacgcgc ggaagtggct cgcgatcacc
      121 ggcaagccgc tcgcggcgac ggccggcgcg atgatcttcc agaagggggg caacgcagtg
      181 gacgctgcgt gcgcgatgat cgccgcgacc tgcacgatgt gggacacgct ctcctggggc
      241 ggcgagacgc aggcgctcat ctacaacccg cacaccggca aggtcgtcgg cgtcaacgcc
      301 ctcggcgtcg cgccgaccgg cgccacgccc gagttctttc acagcaaggg catggcctac
      361 ccgccggagt acggtccgct cgcggccgtg acgcccggca cacccggcgg catcatggtc
      421 atgctcgccg agtacgggaa gctgtcgctc gcggaggtgc tcgcccccgc gatcgagatg
      481 gcggatgggt acccgatcga gcagcagctc gtctcgactg tcacgcgcca gcgagactgg
      541 ttgctcaagt ggcccgagtc gcggcgcgtc ctgctgtcgc atcccggcga ccggccactc
      601 gccgccggcg aagtcttccg acagccggac cttgcggcga cgctgcggaa gctcgtcgaa
      661 gcggagaagc aggcgctcgc ggccggcaag tcccgcaggg acgcgatctt cgcagccaac
      721 gaccgcttct acaaaggcga tatcgcacgc gagttcgtgc gcggctcgca ggagatcggg
      781 ggccttcaca cggtcgagga cctgtccaac tggaaggtcc ggatcgagga gccggtccgg
      841 acgacttaca agggcatcga cgtctacaag ctcgatttct ggacgcaggg gccggcgatg
      901 ctccaggcgt tgaacatcct ggagaccgcg gacgtgaagg cgatgggcta caacagcgcg
      961 cgatacctcc acgcgctgta ccaggcgatg aacctcgcct tcgccgatcg cgacttctac
     1021 tacggcgaca cgtattttcc gcccgaggag ccgcgccggg gtctgctgtc gaaggagtac
     1081 gcaaaagcgc gggccgcgaa gatcgactgg acgaagaacg atccgaacgt gaagccgggg
     1141 gatccctacc ggttccaggg agggaagaac ccgtacgtcg atctgctcga gaaatggccg
     1201 ccgccggcgg cgaaggcgaa agcggagagc gcagggccgg acgcggcgaa ccgcgttccc
     1261 gatttccggg aagcgtttta ttccgggacg acgtcggtcg aggcggccga cgccgagggc
     1321 tgggtcgtct cggtgacccc gtcgggcggc tgggtcccgg cggcgatcgc cggcaagacc
     1381 ggaatcggca tgagccagcg catgcagagc ttcgtcctcg accgcgccga aagcccgttc
     1441 aacgtgctcg agcccggaaa gcatccgcgc gtgacgctga cgcccacgct cgcgctgaag
     1501 gacggcaagc cgtatctctg cttctccgtc cagggcggcg actcgcagga ccagaacctg
     1561 ctgcagttct tcctgaacgt cgtcgagttc ggaatgaccg tgcaggaggc gaccgaggcc
     1621 gcgaacatca acagctacca gatgagaagc tcgttcggcg cgcacgaggc gcggcccggc
     1681 gcgatgatga tccacccgtt gcttcctccc tgggtgcgct cggagctggc ccggatgggc
     1741 tacaagctcg aggtcgcgcc gaagacgtcc ggaccgatca acgcgatctt cctcgaccgc
     1801 gagcacggca cgatgtgggg gggttcctcc gaccacggcg aggactacgg gatcgggtgg
     1861 taggtcgccg gctccgggaa tgaaacgcga cgcgatccgc tggattgcgt tcttcgccgc
     1921 ggtcaagctc ctgtttcact tcttgagcaa cggcctctac ggattccatc gcgacgagct
     1981 ggcaaccctt gatgacgcgc gccatctcgc ctggggctac gtcgcctatc cgccgctgac
     2041 cccgttcctc ggacagatcg agctcgcgct tttcggcgcc acaccgcccg ccttccgcct
     2101 cctcgcggcg cttgcccagt cgatcgcgat cgtgctcacc gcgatgacgg cgaaatgcct
     2161 cggcggcgac cgctccgcgc agtggatcgc ggccatcgcg gtcgccatct cgccgatctc
     2221 gctcgccgcg agcgcactgt ttcagtacgt ggccttcgat tttctctggt gggtactgat
     2281 cgcgtatttc gccgtgcgcc tgatcgagag cgacgacccg cggtggtgga tcgcgatcgg
     2341 aacggtcatc ggcctcggag ccctgacgaa gtacaccatc gccttttacg ttgccggtat
     2401 cgtcgcgggc gtgctcgcga cggacctgcg acggcatctc cggagccgct ggttgtggat
     2461 cggcatggtg gtctcgatcg cgatcgccct gccccacttc ctatgggagg cgaaccacgg
     2521 tttcgtgacg ctcgagttcc tgcggcacgt tcacgcgcgt gacgtccgca tcgggcgtac
     2581 gaaggacttt gtgctcgatc agatccgcgt ggcatcgaat ccggtcacca ttccgctgtc
     2641 catcgtcgga ctgatcgcgg tgctccagtc gcgccgtttc cgaatgctcg cgtggatggt
     2701 gctcgttccg ttcgcgctct tcctcctggc gcaggggcgg ggttactaca ccgggcctct
     2761 ctacccgatc ctcttagcgg ccgggtcggt gatcaccgag cagctcgtga agtcgcgtcg
     2821 gcgcgcggcg tttgcggtga tggccgtgct cgcggcgatc gggtgcgccg tggtcccgat
     2881 cgcgattccg ctcgcgcgac cgcggacgcg tctcttccgg atcgcgagcg ctctcaacca
     2941 tgaccttcgc gaggagttcg gctggcccga gatggcccgc gaggtcgatc gaatcggcgc
     3001 ctcgctctcc cccgaggaac gggcgcattc cgcggcgctc tgcgctaact acggggaggc
     3061 gggcgctatc agcctctacg ggcggacgcc ggtcttgagc ggcgtcaact cgttctgggc
     3121 gcgcggatac ggcaatccgc cgccgcgaac actgatcgtt ctcgggttcg gccgcgaagg
     3181 cctcgagcgc cgattcgccg aagtcgttcc cgccgggcgc atcccgaacc cttacggtct
     3241 cgagaacgag gagtcggagc ggccggagat ctacgtctgc cgcggcctgc gcgagccgtg
     3301 gccggagttc tggcggcggc tccgctcctt cggttgacac cgcgctaggg aatgacctcg
     3361 acgttcggat cgaggacgac gccgtccacg gtgaccttgt aggcgaacgt gccctgggcg
     3421 gccgagggcg tgagcgcggc gcagaacctt cccttgcaga ccagatccgt gaaggggttt
     3481 ccgggcgagg ggttccccgg cttgaaggtg atcttcatcg acacgccgtt ggtcacccag
     3541 acgacgatgt cctgattcat cttgatctgc accttgtccg gtttcaccga gagcgccgtg
     3601 ccggtcttcg tgacctcgac gactttgact ttcgtcttca agtccggcgt cggggtcggc
     3661 gtcggctgca cggtctgcgc gccgagctcc atcgcgggaa gaacgagggc cgaagcgagc
     3721 acgagtgtcc agaaccaccc ggagcgggcg agatgattag cctttcgcat ttctcctccg
     3781 tcgtttccgc gagtctatcg tttgcggctt cccgtcacgt gcggcttttc gcggacgcga
     3841 gcgcctcgcc gaagccgggg agacttcgca ggttcttgag ctcgggctcc cgctcgatct
     3901 gggccgctcc gaggcccgct ttcacggcct tgccaagcca gccgatcgcc tcgtcgctct
     3961 tccccgcgag gtctgcgacg atggccgcgt agaggaagtg gtccgggttg gccggctcga
     4021 tttcgagcgc ccttcgaatg tgcaaccgcg ccggatcctt cctgccgagc ttcgcgtcgc
     4081 agatcgccac ggtcgcgtga gcggccgcgt tgttcgggtt gagcgagagc tcccgctccg
     4141 cgagttgaac cgccttctcg tacgccgccg gcgccttgct ctcgagctcc ggcgtccagc
     4201 gataggcgtc gccgaggttc gcccagtaca ggtacttccc gggcgtgagc ttcgtggcct
     4261 cctcgaaggc ggcggcggca gcgcggtagt tgccgacgta gtactcgaga tttccaaggt
     4321 tcgtgtaggc gccgtcgctc ggattgatcc ggatcgaacc ttgatacgcc tcccgcgctt
     4381 cggcgaatcg atccgccttg aagagcgcgg ctccgaggtt gttgtaggcg cgcacgctgt
     4441 ccggccggcg attgatcgct tcccggaaca tggggatcgc ttctgagtag cgcccgcctt
     4501 ggtagtagaa gaagccgaac tggttgtaga ccgcccagta gctcggctgc agcgccagcg
     4561 cgcggcgata cgtcgcctcg gctctcggat cccgggtcga acggtaggcg tcggccagac
     4621 cgagcagggc gtcgatggaa gagggctgct ccgcgagaac gcgctcgaat tcccgcgcag
     4681 cgtccgcggg cttgcccgtc atcctgagga cctgcccgag cgtcgtgtgc acgtccggaa
     4741 gccggtcgtc gagctttcgt gcggcctcgc aggcggcgat cgcgcgctcc gccgacgcgg
     4801 gatcgcgcgt gatttcgaat tggttcagat gagcacgacc gagcgcggcc agaatgaggg
     4861 gcgagtcggg gtgcttccgg tccagatccg tcaggatacg aatggccgtc gcaatcgatt
     4921 ccggtacgtc gtagcggcgg agcgcgccga tcgccttcag gtaggtctcc tgcgcggcac
     4981 tgctcaacgg caccgttccc gggcgcgccg gagattccgc cctccttttt gcgccgagcc
     5041 aggagaagac gctctcggcc aggcgatcct gcgccccgaa gatttccgat gcggacgtcg
     5101 tcacttcgtc tcccccgatg tgcacgccgt ccggcgctcg tacaagagaa caggtgatcc
     5161 gaagttgatc gccggttcgc tggatggcgc ctgagaggat caggctcgct cccgtcgacc
     5221 gggcgacgcg aacgaagtcc ttctcctcgg cggagaactc gtcagtcg
//
LOCUS       DASWAV010000135         2200 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U2665
            k141_688448, whole genome shotgun sequence.
ACCESSION   DASWAV010000135 DASWAV010000000
VERSION     DASWAV010000135.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960316
            Sequence Read Archive: SRR10260007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2200)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2200)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:47:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,929
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2200
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_688448"
                     /isolate="SMAG_U2665"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.36444 N 132.31889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..384)
                     /locus_tag="VF958_02725"
     CDS             complement(<1..384)
                     /locus_tag="VF958_02725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010959739.1"
                     /note="involved in the processing of the 5'end of 16S
                     rRNA; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rne/Rng family ribonuclease"
                     /protein_id="HEX9148053.1"
                     /translation="MRKELLVNATPPETRVAILEDGRTVEVLHERRGRQGLVGNIYLG
                     RVHRVLPGMQAAFVSIGLERDAFLYVEDVLARSADLDSDGDEEGPRDGGREAADRPRI
                     DDLLKEGQELVVQVTKDALGGKGPRV"
     gene            complement(490..1587)
                     /gene="rodA"
                     /locus_tag="VF958_02730"
     CDS             complement(490..1587)
                     /gene="rodA"
                     /locus_tag="VF958_02730"
                     /inference="COORDINATES: protein motif:HMM:TIGR02210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein RodA"
                     /protein_id="HEX9148054.1"
                     /translation="MIRSVLPVPRRLDLMILCCALSLAVLGILFVSSTTHGGRFDGLA
                     TRQAIWVLVGLGGLVAAVLFDYRILLKFSFFIYCVSLVPLVYLLFFGERIANVKSWIR
                     VGTFQFQPAELAKIATALLLAYLFENEDDAKMKGSTFLKVAVIVAVPFLLVFLQPDAG
                     LAATFLPLAAMALFFGGLPIRAWVGILVAVALTAAIGWFVLADYQKQRIGTFLNPESD
                     VLGSGYQVRQSKIAVGSGGFAGKGFRSGTQSQLRFLPVQHTDFILAVIAEEWGFLGIC
                     LVLALFAVLLLRALTLARVARDRGGVFLILAIASMLFFSVIVNASMMIGLLPTTGIPL
                     PLVSYGGSSVATSFVGIGLILAVEYRRFANA"
     gene            complement(1584..>2200)
                     /locus_tag="VF958_02735"
     CDS             complement(1584..>2200)
                     /locus_tag="VF958_02735"
                     /inference="COORDINATES: protein motif:HMM:NF013100.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HEX9148055.1"
                     /translation="ARTKRGARWYPSETISVAIGQGPLLMTPMQVARAVSALVTGGKL
                     PTPHLFFASQDPKMGVRLRYLAQPRQSLAIDPALLAIVKNGMWAVVNEPGGTAFGSRV
                     PGVEAGGKTGTVQVARRDAVLREGVDRSRLEDHAWFTAFAPIDDPRIVVVVFVEHGGH
                     GSSAAAPIARRLLAKYFGVDVAPRPGEPGFARAASKIPGAEVVE"
BASE COUNT          414 a          741 c          745 g          300 t
ORIGIN      
        1 cacgcgcggg cctttcccgc cgagagcatc cttggttacc tggacgacga gctcctggcc
       61 ctccttcagc aggtcgtcga tcctcggccg atcggcggcc tcgcgccccc cgtcgcgcgg
      121 accctcctcg tccccatcgg agtcgagatc tgccgaccgg gcgagcacgt cctcgacgta
      181 gaggaacgca tcgcgctcga ggccgatcga gacgaacgcg gcctgcatcc cgggcagcac
      241 gcggtggaca cggccgaggt agatgttgcc gacgagaccc tggcggccgc ggcgctcgtg
      301 caggacttcc acggtgcgac cgtcctccag gatcgcgacc cgcgtctccg gaggcgtggc
      361 gttgaccagg agctctttac gcacagagct cgatcgcggc gcgcgcgaag cgagggcgcc
      421 gcctcaagta ctccctctcc ctccgggaga gggttggggt gaggggtgca gagagtcgcc
      481 ggtcttcttt caagcgttcg cgaagcgacg gtactccact gcgaggatca atccaatccc
      541 gacaaacgac gtcgcgaccg acgagccgcc gtacgagacg agcgggagcg ggatcccggt
      601 ggttggaaga agcccgatca tcatcgacgc gttcacgatg accgagaaga aaagcatcga
      661 ggcgatcgcg aggatgagga agactccccc ccggtcccgg gccacgcgcg cgagcgtgag
      721 cgctcgtaag agcagcacgg cgaagagggc gagcacgagg cagattccga gaaatcccca
      781 ctcctcggcg atcacggcga ggatgaagtc cgtgtgctgg acgggcagga accggagctg
      841 gctctgcgtc cccgatcgga accccttccc ggcgaagccc ccggagccca ccgcgatctt
      901 cgactgccgc acctggtatc ccgatccgag aacgtccgac tccgggttca ggaaggttcc
      961 gatcctctgc ttctggtaat cggccagcac gaaccagccg attgcggcgg tgagcgccac
     1021 ggcgacgagg atcccgaccc aggcccggat cggaaggcca ccgaagaaga gcgccatcgc
     1081 ggcgagcggg agaaacgtcg ctgcaagccc cgcgtccggc tgcagaaaca cgagcaggaa
     1141 cggcacggcg acgatcacgg cgaccttcag gaaagtcgag cccttcatct tcgcgtcgtc
     1201 ctcgttttcg aacaggtacg cgaggaggag cgcggtcgcg attttggcga gttccgccgg
     1261 ctggaactga aaggtgccga ctcggatcca cgacttcacg ttcgcaatcc gctccccgaa
     1321 gaagagcagg tagacgagcg gaacgaggct gacgcagtag atgaagaagg agaacttcaa
     1381 caggatccgg tagtcgaaga ggacggccgc gacgagcccg ccgaggccca cgagcaccca
     1441 gatcgcctga cgcgtggcaa ggccgtcgaa ccggccgccg tgggtcgtgg acgatacgaa
     1501 cagaatcccg agcaccgcaa gcgacagggc gcagcagagg atcatgaggt ccaggcggcg
     1561 cgggaccggg aggacgctcc ggatcattcc acgacctccg cgcccgggat cttggacgcc
     1621 gcgcgcgcga accccggctc tccgggccgc ggagccacgt cgaccccgaa gtacttcgcg
     1681 agaaggcgtc tcgcgatcgg cgctgcggcg ctcgagccgt gcccgccgtg ctcgacgaag
     1741 accacgacga cgatgcgcgg atcgtcgatc ggcgcgaacg ccgtgaacca cgcatggtcc
     1801 tccaggcgac tgcgatcgac cccttcgcgg agaaccgcgt cgcgccgcgc gacctgaacg
     1861 gtcccggtct tgcccccggc ctcgacgccg ggaacgcggg agccgaaggc ggtgccgccc
     1921 ggctcgttga ccaccgccca cattccgttt ttcacgatcg cgaggagcgc gggatcgatc
     1981 gcgagcgact gcctcggctg ggcgagataa cgcaggcgga cgcccatctt cgggtcctgc
     2041 gaggcgaaaa agaggtgggg cgtcggcagc tttccaccgg tgacgagcgc cgagactgcg
     2101 cgcgcgacct gcatgggcgt catgagcagc ggtccctgtc cgatcgcgac cgagatcgtt
     2161 tcgctcggat accagcgcgc gccgcgcttg gtgcgggccc
//
LOCUS       DASWAV010000136         1513 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U2665
            k141_88342, whole genome shotgun sequence.
ACCESSION   DASWAV010000136 DASWAV010000000
VERSION     DASWAV010000136.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960316
            Sequence Read Archive: SRR10260007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 1513)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1513)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:47:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,929
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1513
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_88342"
                     /isolate="SMAG_U2665"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.36444 N 132.31889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..460
                     /locus_tag="VF958_02740"
     CDS             <1..460
                     /locus_tag="VF958_02740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9148056.1"
                     /translation="ARGSTSRRRPTPGPDEIPLLTSRDLEPRLRSGAGDTGRDLFDLR
                     EPTPPPPKPTPAPTPPPGPGHPAFIGPLPPPPPTPTPAPPDPPFKLIGIFGPKEHPIA
                     VLANGDDIFNARAGETVLGRWILKKVGYESIDVGFVGFPPTETRRLPITQ"
     gene            489..>1513
                     /locus_tag="VF958_02745"
     CDS             489..>1513
                     /locus_tag="VF958_02745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9148057.1"
                     /translation="MRKRIHPSAAIVVALTLLVGCTSSQAFREARQEEAVEHWDLAVL
                     KYARAAELEPKNRAYRIALARARIKASQFHFEKGKMYRNSGRPELAVVELNQAVLLDA
                     TNQYAEVELRKAREEAAKVVAERTGETRIEALKRKVRGSRPKPPLLEPSSDKPINLNF
                     PQQKPVKQIYRALADAAGINVIFDPQLKDDNVSIVLANIEFQKALETLLRQENHFYKV
                     IDERTILIAADTPQNRKTYEDLVIRTFFLSNADVTEVANALRALLQTTRNSVNKAEYS
                     VTLRDTADKVAVAERIVEQNDKQVAEVVVDVELLQINADKAQTLGLELVPRSVGATIP
                     AAGGTTNL"
BASE COUNT          286 a          542 c          475 g          210 t
ORIGIN      
        1 ggcccgcggc agcacatccc gccggcgtcc cactcctggt cccgacgaaa ttcccctcct
       61 cacgtcgcgc gatctcgagc cgcgtctgcg cagcggcgcg ggcgacaccg gacgcgacct
      121 gttcgacctg cgggagccga cgcctcctcc tccgaagccc acaccggcgc cgaccccccc
      181 tccgggcccg gggcatcccg cgttcatcgg cccgctgccg ccgccccctc cgacgcccac
      241 gccggcgccg ccggatccgc ccttcaagct gatcgggatc ttcggtccga aggagcaccc
      301 gattgccgtt ctcgcgaacg gcgacgacat cttcaacgcc cgcgcggggg agaccgttct
      361 ggggcgatgg atcctcaaga aagtcggcta cgagtcgatc gacgtcggtt tcgtcggctt
      421 tccgccgacg gaaacgagaa ggctgcccat tactcaatga gccggaaacg agaaaggtcc
      481 ctatcgcgat gagaaaacga attcacccga gtgccgcgat cgtcgttgcg ttgaccctcc
      541 tcgtggggtg cacgagctcc caggcgttcc gggaagcgcg tcaggaggag gcggtcgagc
      601 actgggacct cgcggtcctg aagtacgcgc gcgccgcgga gctcgagccg aagaaccgcg
      661 cctaccggat cgcgctcgcc cgggcgagga tcaaagcgtc gcagttccac ttcgagaagg
      721 ggaagatgta tcgcaattcg gggcggccgg agctcgccgt ggtcgagctc aatcaggcgg
      781 tcctcctgga cgcgaccaac cagtacgccg aggtggagct tcggaaggcg cgagaggagg
      841 cggcaaaggt cgtcgcggag cggaccggcg agacccgcat cgaagctctc aagcgcaagg
      901 tgcgcggctc ccggccgaaa ccccccctcc tggagccctc gagcgacaag cccatcaacc
      961 tgaactttcc gcagcagaag ccggtcaagc agatctaccg cgccctcgcc gacgccgccg
     1021 gcatcaacgt catcttcgac ccgcagctga aggacgacaa cgtctcgatc gtcctggcga
     1081 acatcgagtt ccagaaggct ctcgagacgc tgctccgcca ggagaaccac ttctacaagg
     1141 tcatcgacga gcggaccatc ctgatcgcgg ccgacacgcc ccagaaccgg aagacctacg
     1201 aggacctggt catccggacc ttcttcctct cgaacgccga cgtcaccgag gtggccaacg
     1261 cgctgcgggc gctcctccag accacccgca actcggtgaa caaggccgag tattccgtga
     1321 cgctccgcga tacggcggac aaggtcgccg tcgccgagcg gatcgtcgag cagaacgaca
     1381 agcaggtggc cgaggtcgtc gtggacgtcg agctcctgca gatcaacgcg gacaaggcgc
     1441 agacgctcgg cctcgagctg gtgccccggt ccgtcggcgc gacgatcccc gccgccggag
     1501 ggacgacgaa cct
//
LOCUS       DASWAV010000137         2817 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U2665
            k141_168443, whole genome shotgun sequence.
ACCESSION   DASWAV010000137 DASWAV010000000
VERSION     DASWAV010000137.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960316
            Sequence Read Archive: SRR10260007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2817)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2817)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:47:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,929
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2817
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_168443"
                     /isolate="SMAG_U2665"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.36444 N 132.31889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..651
                     /locus_tag="VF958_02750"
     CDS             <1..651
                     /locus_tag="VF958_02750"
                     /inference="COORDINATES: protein motif:HMM:NF039288.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HEX9148058.1"
                     /translation="PAVNLPFRGPKDAGGAASWMQVGERTGRFLSCVAGERLTSLTVE
                     TWGLPEDFLRPVAVASVKGALESHTPETVNFVNALFVARDRGLSVSETRHEEPGNYAR
                     SIRVALSDGQRTASAHATLFSGSDARVVEVDGLPLEFRPEGTVVFLRNRDVPGVVGNV
                     GTILGEAGINIANFSLARGPGDRAAAVIAVDSVPTSDVLARLRGAPGVEEVRVVTW"
     gene            648..1718
                     /locus_tag="VF958_02755"
     CDS             648..1718
                     /locus_tag="VF958_02755"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018180357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase family protein"
                     /protein_id="HEX9148059.1"
                     /translation="MSRLKVVVLYGGRSVEREVSRVSARTIVQALDRAKYEVVPLAVD
                     EAGHFLSSPDSSRLLAEGPVPDRFRRAEPAGEAIVPVEISPGRADVVFPIIHGATGED
                     GILQGFLDFLGIPYVGAGVTGSAIGMDKAVFKALLASAGIPTARSVVVRTSERSLSRE
                     AIAGLGLPLFVKPATGGSSVGVSKVKDLRQLDAALDHAFRYDDRALVEQGIDARELEC
                     AVLGDEEPRASIPGEVVPGHEFYDYDDKYREDKARLLIPAPVSDRIAEEVRRLAVEVF
                     RLCGISGMARVDFFLERGTDRVLVNELNTLPGFTAISMYPKLWEASGVPLPALLDELI
                     ALALARRERRARLSHEPPAELE"
     gene            1769..2740
                     /locus_tag="VF958_02760"
     CDS             1769..2740
                     /locus_tag="VF958_02760"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF019334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEX9148060.1"
                     /translation="MALERGSSIGSYRVLFRLGSGGMGEVWRGRDDRRNLDVAIKVLS
                     PDSARDADRMRRFAMEAKAASSLNHPNILTIYEVGESDAGPYLVTEYVDGDTVRGLLN
                     DGALPLEKAIDVALQAAEGVASAHAVGIVHRDLKPENLMVNREGRVKILDFGLAKHLR
                     PGEEAMDVARTATGMIIGTAGYLSPEQLRGGKASERSDVFALGLVLYEMLTGDNPFRR
                     NNAVDTFTAIMRDDPPPLPEKLGPASSHVVPVLARALSKKPEDRYPSAVELASDLRAV
                     QSRIAPPPVPTEVAAGDSRPARAPWGILIAAGLLALGAAAFLLLRGC"
BASE COUNT          420 a          964 c          959 g          474 t
ORIGIN      
        1 cccgccgtca accttccgtt ccgcgggccg aaggacgcgg gcggcgccgc ctcctggatg
       61 caggtcggcg agcggaccgg acgcttcctc tcctgcgtcg cgggggagcg tctcacgtct
      121 ctgacagtcg agacgtgggg cctgcccgag gactttcttc gcccggtcgc agtcgcttcg
      181 gtcaagggcg cgctcgaatc gcacacgccc gagaccgtga acttcgtcaa cgcgctcttc
      241 gtcgcgcgcg accgagggct ctccgtttca gagacgcgac acgaggagcc cggcaactat
      301 gcccggtcga tccgggtggc gctctccgac gggcagagga cggcgagcgc tcacgcgacg
      361 ctcttctccg gcagcgacgc gcgcgtcgtc gaggtcgacg ggctcccgct cgagttccgc
      421 cccgaaggca ccgtcgtttt cctgagaaac cgcgacgtgc ccggcgtcgt cggcaacgtc
      481 ggaacgatcc tgggagaagc cgggatcaat atcgcgaact tctccctggc ccgcgggccc
      541 ggggaccggg cggcggccgt catcgcggtc gattcggttc cgacatctga cgtcctcgcc
      601 cgcctgcgag gtgctccggg agtggaagaa gtgcgggtcg tcacctggtg agccgcctca
      661 aagtcgtcgt cctgtacgga ggccggtccg tcgagcggga ggtctcgcgc gtttcagcgc
      721 gaacgatcgt ccaggcgctc gatcgcgcca agtacgaagt ggtgccgctc gcggtcgacg
      781 aagcggggca tttcctctcc tcaccggact cctcacgcct gctcgcggaa ggccccgttc
      841 ccgaccgttt tcggcgggcc gagccggcgg gggaagcgat cgtgccggtg gagatttctc
      901 cgggacgggc ggacgtcgtc tttccgatca tccacggcgc gaccggcgaa gacgggatcc
      961 tccagggatt tctcgacttc ctcgggattc cctacgtggg ggcgggagtc accggctcgg
     1021 cgatcgggat ggacaaggcg gtgttcaagg cgttgctcgc gagcgcgggg attccgacgg
     1081 cgcggtcggt cgtcgttcgg acgtccgaac ggagcctctc ccgcgaagcc atcgcgggcc
     1141 tcggactgcc tctcttcgtg aagcccgcga ccggaggttc atcggttggg gtcagcaagg
     1201 tcaaggatct ccgtcagctc gacgccgccc tcgaccacgc gttccgctac gacgaccgcg
     1261 cgctcgtcga acaggggatc gacgcgcgcg agctcgagtg cgccgtgctc ggcgacgagg
     1321 agcctcgagc ctcgatcccg ggcgaggtcg tgcccggaca cgagttctac gactacgacg
     1381 acaagtaccg cgaggacaag gcccgcctgc tgatccctgc tccggtatcc gatcggatcg
     1441 cggaggaagt gcggcgtctc gccgtcgaag tgtttcgtct ctgcgggatc tccggaatgg
     1501 cgcgggtcga cttctttctc gagcgcggga cggaccgcgt cctcgtcaac gagctgaaca
     1561 cgctccccgg tttcacagcg atttcgatgt acccgaagct ctgggaggcg agcggagttc
     1621 cgctccccgc actgctcgac gagctcatcg ctctcgcact cgcccgtcgg gaaaggcgcg
     1681 cgcgcctgtc ccacgaacct cctgccgagc tcgagtagga accctgatcg ccggcgctgc
     1741 gctctcactc ggttaggctc tgcgccggat ggctctggaa cgcggcagct cgatcggctc
     1801 ctaccgggtc ctcttccgtc tgggttcggg gggcatgggc gaagtgtggc ggggccgcga
     1861 cgaccgtcgg aatctcgacg tcgcgatcaa ggttctgtcg cccgactccg cccgcgacgc
     1921 cgatcgtatg cgccgcttcg ccatggaggc gaaagcggca tcgtctctca accaccccaa
     1981 catcctgacg atctacgagg ttggggagag cgatgcggga ccttacctcg tcaccgaata
     2041 cgtggatgga gatacggttc ggggactcct gaacgacggc gcgctgccgc tcgagaaagc
     2101 catcgacgtg gcgctccagg cggcggaggg ggtcgcgagc gctcacgcgg tggggatcgt
     2161 ccaccgcgat ttgaagcccg agaacctcat ggtcaaccgc gagggacgcg tcaagatcct
     2221 cgacttcggc ctcgcgaaac accttcgccc cggcgaggaa gcgatggacg tcgcgcgcac
     2281 ggcaaccgga atgatcatcg ggaccgcggg gtacctttcg cccgaacagc tccggggggg
     2341 gaaggcgagc gagcgctcgg acgtcttcgc cctcggtctc gtcctctacg agatgctgac
     2401 gggcgacaac cctttccggc ggaacaacgc ggtcgacacc ttcaccgcga tcatgcgcga
     2461 cgatccgccg cccctgccgg agaagctcgg ccccgcgtcg agccacgtgg ttcccgtcct
     2521 tgcccgggct ctctccaaga agcccgagga tcggtacccg tcggcggtgg agcttgcgtc
     2581 agaccttcgg gcagtacagt cccgcatcgc gccgccgccc gtcccgaccg aggtcgcggc
     2641 gggcgactcg cgtccggctc gcgcgccgtg gggaatcctc atcgcggccg ggctccttgc
     2701 gctcggggcc gccgcgttcc ttctgctccg aggttgctag aggctcgccg gctggttcta
     2761 cgtccgggaa gcgttcccct ctcgcatgcg aaccggggac gccggggtgc gatcgag
//
LOCUS       DASWAV010000138         5275 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U2665
            k141_766147, whole genome shotgun sequence.
ACCESSION   DASWAV010000138 DASWAV010000000
VERSION     DASWAV010000138.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960316
            Sequence Read Archive: SRR10260007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 5275)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5275)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:47:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,929
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5275
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_766147"
                     /isolate="SMAG_U2665"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.36444 N 132.31889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..4109)
                     /locus_tag="VF958_02765"
     CDS             complement(<1..4109)
                     /locus_tag="VF958_02765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9148061.1"
                     /translation="MSSPVLRRVRLTSALSLSALLVFAASAIGDDKDLLVRTDSPTNL
                     MIIFGNSQTTQQPILGATSAWDGDADSPASKMGASKRVVARFVADKAGQFNFGLTSFA
                     HNPNAGSITIFRKHWLYAPLTVDYPAETWKEPAGTIERWGDLGSGPCTNLVVPSCTDR
                     SPSFITLPATATTSGPFFGAQGNGIAFIYLDGGSSSATRRIKITLKTGEYGDAYTDGS
                     LSAYSIQSPGPPHSMEVEKEYQEKSGGNWGTPATTPNGSPAKVTVFYIPPTSLPTDLF
                     FQSDPDGGKAIGFLDDRQTDFTVSANCSGWEFQVNSAPLPLVKIPRDYLWGSTCKPAQ
                     NSTACIARMMRPQAELVRYDQGSGTYVITDHDNPGYGGSGNKYADGCNPELLGAVDTG
                     LDITENQTILTTRNGGQAPIKDLLDNIYDYFSNPAVDGFQNGKRIDDPNKACRNSAVI
                     LIYDNFNGCQNDNCSFLTSHVLKALKQIGVPVYVIGLGSGAVATSDTGRCIAQNSGAI
                     LPDGSVGYFPVTSAAGLYQALADITSYVNESSKDFASSTVSSVQTGGEQMVYLATFNA
                     AKNRSIWNGRVNGYKLDASGNIQMGPKTIRDPNDPDNGLMLLAPSNDPSSLIWNAGEN
                     LADTPGTGATDPTAVLAPGAGMSSSSYVDSSNDTVTTIPTHFYPGRKIVFSLPPGYTD
                     PVTQLPIPPSDTVPENRNDLTYTTSASWWPALKALMGPQTSPPGVVTPSIADADAGNA
                     LRFIWGDRDAVMTTTEANQRYLGLKLGDTFHSNPLIVGRPADFSFYNRNLNNYQAFFK
                     TYRSRRRVLYAGANDGLLHAFEIGVFNRDATVCSTLSDGSLPPCYDLGTGVELFAYAP
                     RAIMQIFKPLKDAAGPQNKKMEWTVDGAPSAFDAFIDGSHNGTPTASERAWHTVLVGG
                     MREGSPFEGTSGASPKASRGTYYALDVTQPDELVDDGSGGVTGPSTPGTYLAPKCLNA
                     SGDASCGKDAADSTVRSGQPARAWPTVMWEIADTGDLDASGSPGFGYGDMGETWSKPA
                     LGRVRVCLTNCGSTSAPLPTTIDVYVAIFGGGFDRERLNRRGNWLYMVDVETGKTLYR
                     VNSSCGVNAGAAGCTPKYFGSISSEPTALDLNGDGYLDFVYVGDLKGQMWRIDLTDLR
                     RLASPPGLRWNNQLDLVSGSGKPFLLFQAPQPVPPAVDPFYPIYFRPTAVSLGFSSSG
                     KPSLGIAFGTGDRDDIISTVDPLSLNFRQRFYYVIDDSNATTRTESDLLDIPSASAPP
                     ATSIPTNGWFIEFVEGERLVTDSLAIKGLIFFGTFNPIPTRDARDACNNLIKCVNQRG
                     LARFYTVNYSTGDPGPGALGSDRGETQQHASFLTNPVFYNESSVIYTTD"
     gene            complement(4106..4600)
                     /locus_tag="VF958_02770"
     CDS             complement(4106..4600)
                     /locus_tag="VF958_02770"
                     /inference="COORDINATES: protein motif:HMM:NF025699.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilX N-terminal domain-containing pilus assembly
                     protein"
                     /protein_id="HEX9148062.1"
                     /translation="MRSRPIGIGRREEGFALFLSMLLLLVLTVLGIALLFTATTEQSL
                     SGNETKVSKVFYAACSGVDYATAKLAVDKNYVGGLMPVGISSHYPGMTGDIQVTLTRP
                     INVGYSVHLGDQFESRGSTYESNQIVENFYNFTSTAQGSAIQAAKMVTADVGIYPQQR
                     RIQQ"
     gene            complement(4597..>5275)
                     /locus_tag="VF958_02775"
     CDS             complement(4597..>5275)
                     /locus_tag="VF958_02775"
                     /inference="COORDINATES: protein motif:HMM:NF027400.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0043683 - type IV pilus assembly [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="PilW family protein"
                     /protein_id="HEX9148063.1"
                     /translation="ADTFTFSMNPQDSGAQKLNAAPGGAPALEQPYAGGAVDDILFFV
                     DEGKSNASSSTVDTHPSLAQATFDPSSGRYDIQPLVDEVEDFQVAYGVDGIVPGNPPD
                     RGISPVLVDASAVNKDEWVGNVASEVSTDLVISATDPKRVDAFLDTTIPSGSPNPAPA
                     TPALRSIWLSLVVKASDPDMKYDGPGATGIKILDSTAVSFSDSAITGRRYRRRVQSIA
                     VALRNYQ"
BASE COUNT          901 a         1589 c         1779 g         1006 t
ORIGIN      
        1 ttgtccgtcg tgtagatgac gctcgactcg ttgtagaaga cggggttcgt gaggaagctc
       61 gcatgctgct gggtctcccc tcgatcggat ccgagcgccc cggggcccgg atctcccgtc
      121 gagtaattga ccgtgtagaa acgagcgagt cctcgctggt tgacgcactt gatgaggtta
      181 ttgcaggcat cgcgcgcgtc gcgcgtggga attggattga aggttccgaa gaagatcagc
      241 cctttgatcg cgagcgaatc tgtcaccaga cgctcgcctt cgacgaattc gatgaaccag
      301 ccgttcgtcg gaatcgacgt cgcgggcgga gcgctcgccg acggaatgtc gagtagatcg
      361 ctctcggtcc gagtggtcgc gttcgagtcg tcgatgacgt agtagaaccg ctgccggaag
      421 ttcaggctca ggggatcgac ggtcgagatg atgtcgtccc ggtcccccgt tccgaaggcg
      481 attcccagac tgggcttgcc gctcgagctg aacccgaggg acaccgcggt cggacggaag
      541 tagatcggat agaacgggtc gaccgccggg gggaccggtt gaggtgcctg gaagagcagg
      601 aatggctttc cggacccgct gacgagatcg agctggttgt tccaccggag gcccggcggg
      661 ctcgcaagcc ggcgcaggtc ggtcaggtcg atccgccaca tctgaccctt caagtccccg
      721 acgtaaacga agtcaagata tccgtctccg ttcaggtcga gtgccgtcgg ctccgaagag
      781 atcgagccga agtacttggg cgtgcatccg gctgcgccgg cgttgacccc gcagctcgag
      841 ttgactcgat agagggtctt gccggtctcg acgtcgacca tgtagagcca gttgccgcga
      901 cggttcagcc gttcgcggtc gaagccacct ccgaagatcg cgacgtacac atcgatcgtt
      961 gttggcagcg gcgcgctcgt gcttccgcag ttggtcagac agacccggac ccgtccgagc
     1021 gcaggcttgg accaggtctc gcccatgtcg ccgtagccga agccggggga gcccgaggcg
     1081 tccaggtcgc cggtgtccgc gatctcccac atgacggtcg gccaggctcg cgcgggctgg
     1141 cccgatcgca cggtcgaatc cgccgcgtcc ttgccgcagc tcgcgtcgcc gctcgcgttc
     1201 aggcacttcg gcgcgaggta agtgccggga gtggacgggc cggtgacgcc gccgcttcca
     1261 tcgtcgacga gctcgtccgg ctgcgtgacg tccagggcgt agtaggtccc tcgggaagcc
     1321 ttcgggctgg ctccgctcgt cccttcgaac ggagatcctt cccgcatccc gccgaccagc
     1381 accgtgtgcc aggcccgctc cgatgccgtc ggagtgccgt tatggcttcc gtcgatgaag
     1441 gcgtcgaacg cggatggtgc cccatccacg gtccactcca tcttcttgtt ctgcggtccg
     1501 gcggcgtcct tcaggggctt gaagatctgc atgatcgcgc ggggggcata ggcgaagagc
     1561 tcgaccccgg tccccaggtc gtagcagggg ggcaggctcc cgtcggaaag ggtcgagcac
     1621 actgtcgcat cccggttgaa cacgccgatc tcgaacgcgt gcaacagtcc gtcgttggcg
     1681 ccggcgtaca gcacgcggcg tctcgagcgg taggtcttga agaacgcctg gtagttgttc
     1741 agatttctgt tgtagaagga aaagtcggcg ggacgtccca cgatgagcgg gttcgagtgg
     1801 aaggtgtcac cgagcttcaa cccgaggtac cgctgattcg cctcggtcgt cgtcatgacg
     1861 gcgtcgcggt ccccccagat gaaacgcagg gcattgccgg cgtccgcgtc cgcgatcgac
     1921 ggagtcacga cgccgggcgg gctggtctgg gggcccatga gcgccttcaa cgccggccac
     1981 caggacgcgc tcgtcgtgta ggtcaggtcg ttgcggttct ccggcaccgt gtccgacggc
     2041 gggatcggca gctgtgtcac gggatcggtg tatcccgggg gaagcgagaa aacgatcttg
     2101 cgccccgggt agaagtgcgt cgggatcgtc gtaactgtgt cgttcgagct gtcgacgtat
     2161 gagctggaag acatcccggc gcccggcgcg agcacggccg tcgggtccgt cgcaccggtc
     2221 cccggcgtgt ccgccagatt ctcgccggcg ttccagatga gcgaggacgg atcgttcgac
     2281 ggagccagca gcatcaggcc gttgtccgga tcgttggggt cgcggatcgt tttcggaccc
     2341 atctgaatgt tgccgctcgc gtcgagcttg taaccgttga cgcggccgtt ccagatcgac
     2401 cgattcttcg cggcgttgaa agtcgcgaga tagaccatct gctcgccgcc ggtctggacc
     2461 gacgacaccg tcgacgaggc gaaatccttc gacgactcgt tgacataaga ggtgatgtcg
     2521 gcgagggcct ggtagaggcc ggccgcgctg gtcaccggaa aatatccgac agacccgtcg
     2581 ggaaggatcg ctccggagtt ctgcgcgatg caccggccgg tatccgacgt tgcgaccgcg
     2641 cccgagccga gcccgatcac gtacaccggc acgccgatct gcttcagcgc tttcagaaca
     2701 tggcttgtca agaagctgca gttgtcgttc tggcagccgt tgaagttgtc gtagatcaga
     2761 atgaccgcgg agttgcggca cgctttgttc gggtcgtcga tccgcttgcc gttctggaag
     2821 ccatcaactg ccgggttgct gaagtagtcg tagatgttgt cgaggaggtc cttgattgga
     2881 gcctggccgc cgttgcgcgt ggtgagaatg gtctggttct cggtgatatc gaggccggtg
     2941 tcgaccgcgc ccagcagctc ggggttgcac ccgtcggcat acttgtttcc cgatccgccg
     3001 tagcccgggt tgtcgtggtc cgtaatgacg tacgtcccgc tgccctggtc gtagcgaacg
     3061 agctccgcct ggggccgcat catccgggcg atgcaggcag tcgagttctg ggcgggctta
     3121 caggtcgaac cccagagata gtcgcgcggg atcttgacca gcgggagcgg cgccgagttg
     3181 acctggaact cccagccgga gcagttcgcg ctgacggtga agtcggtctg gcgatcgtcc
     3241 aagaacccga tcgcctttcc gccgtccggg tcgctctgga agaagagatc ggtcggcaac
     3301 gacgtcgggg gaatgtagaa caccgtgacc ttcgcgggac taccgttcgg cgtggtcgcc
     3361 ggtgtgcccc agttgccgcc cgacttctcc tggtactcct tttcgacctc catggaatgg
     3421 ggaggccccg gactctgaat cgagtaggcg gacagacttc catcggtata ggcgtcgccg
     3481 tattccccgg tcttgagcgt gatcttgatg cgcctggtcg cggagctcga gccgccgtcc
     3541 aggtaaatga aagcgatccc attcccctgc gctccgaaaa agggtccgga ggtcgtcgcg
     3601 gtcgcgggaa gggtgatgaa cgagggggag cggtcggtgc acgagggaac caccaggttc
     3661 gtgcacgggc cgcttcccag gtcgccccat ctctcgatcg ttcccgccgg ctccttccag
     3721 gtctctgccg ggtagtccac cgtcagaggc gcatacagcc agtgcttgcg aaagatcgtg
     3781 atcgagcccg cgttcggatt gtgtgcgaag ctcgtcagac cgaagttgaa ctgccccgcc
     3841 ttgtcggcca cgaaacgagc gacgactcgc ttggacgccc ccatcttgct cgcgggcgag
     3901 tcggcgtcgc cgtcccaggc gctcgtcgcc cccagaatcg gctgctgcgt cgtctgggag
     3961 ttcccgaaaa tgatcatcag gttggtcgga gagtccgtcc tcacgaggag gtctttgtcg
     4021 tcgccgattg cggaggccgc aaacacgagc agagccgaca acgacagggc ggacgtcaga
     4081 cggacgcggc ggagtacagg ggagctcatt gctggatcct tcgctgctgt gggtagatcc
     4141 cgacgtcggc cgtaaccatc ttggcggcct gtatggcgct gccctgggcc gtcgacgtga
     4201 agttgtagaa gttctctacg atctgattcg attcgtaggt cgagccgcgc gattcgaatt
     4261 gatcaccgag gtgcaccgaa taaccgacgt tgatcggtcg ggtgagcgtg acctgaatgt
     4321 cgccggtcat tcccgggtag tgactcgaaa tgccgaccgg catcaggcca ccgacgtaat
     4381 tcttgtcgac cgcgagcttc gcggtcgcgt aatccacccc ggaacacgcc gcgtagaaga
     4441 ctttcgagac cttggtttcg ttcccggaga ggctctgctc cgtcgtggcg gtgaaaagaa
     4501 gcgcgatccc gagtaccgtc aggacgagaa ggagtagcat cgagagaaag agcgcgaagc
     4561 cctcttctcg ccgtccgatt ccgatcggac gtgacctcac tggtagttcc tcagggccac
     4621 ggcgattgac tgcacgcgtc ggcgatagcg acgcccggtg atggcggagt ccgagaaaga
     4681 gacggcggtc gagtcgagaa tcttgatgcc ggtcgcgccg ggcccgtcgt acttcatgtc
     4741 cggatcgctc gctttgacga cgagagacag ccagatggag cgaagcgccg gggtcgcggg
     4801 ggcgggattc ggcgacccgc tcggaatcgt cgtgtcgagg aacgcgtcca cgcgcttggg
     4861 atcggtcgct gagatcacga ggtcggtcga gacctcgctc gcgacgttgc cgacccactc
     4921 gtccttgttg acggctgagg catcgaccag gacgggcgag atcccgcgat cgggaggatt
     4981 gccgggcacg attccgtcga ccccgtaagc gacctggaag tcctcgacct cgtccaccag
     5041 aggctggatg tcgtatcgac cggaggacgg gtcgaaggtc gcctgcgcca aggagggatg
     5101 tgtatcgacc gtcgagctgc tggcgttcga tttaccctcg tcgacgaaga agagaatgtc
     5161 gtcgaccgct ccgccggcgt aaggctgttc gagagccggc gctccgccgg gcgccgcgtt
     5221 caacttctgg gcgcccgagt cctgagggtt catcgagaag gtgaacgtat cggcg
//
LOCUS       DASWAV010000139         1587 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U2665
            k141_7782, whole genome shotgun sequence.
ACCESSION   DASWAV010000139 DASWAV010000000
VERSION     DASWAV010000139.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960316
            Sequence Read Archive: SRR10260007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 1587)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1587)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:47:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,929
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1587
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_7782"
                     /isolate="SMAG_U2665"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.36444 N 132.31889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(9..1166)
                     /locus_tag="VF958_02780"
     CDS             complement(9..1166)
                     /locus_tag="VF958_02780"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HEX9148064.1"
                     /translation="MAIQESPTLAVLTKATALVSRGVDVVDFGPGEPDFGTPKNISDA
                     GRRAIEQGFTKYTNSSGMKPLRDAIAERYNRRYGTSLQMEHVITGNGGKQELFNAILS
                     LVTDGDEIIIPSPYWVSFPDQVLFAGGKPVFAPADPQNHFRPAFADIERVSSKRTRGV
                     IINSPCNPTGAVIAEAELEKIVEWCVDRDLCLIFDETYELFVYEGNRHASAMSWFDRY
                     PENIIVVNSMSKTFAMTGWRLGYAVAHPEIIGAMGRIQSHATSNPSSIAQMAAIEALS
                     GDESSVTRMYEAYCERRAWLVPALNAVTGFCCSAPDGAFYVFPEVKQFFGRRGITDSQ
                     SFANFLLDEARVAVVPGSAFGNDDYVRISYATSMDRIREGVSRIAEAVAKL"
     gene            complement(1265..>1587)
                     /locus_tag="VF958_02785"
     CDS             complement(1265..>1587)
                     /locus_tag="VF958_02785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9148065.1"
                     /translation="PIIFVISAGDLRPLVFDEMQRHEILLTILQSFLGFLLLLNLELR
                     FHEALVLFVFWLTQFLFPATRHVMIYVYLAWCLIEMGRMLIWERHVPPAWAGLRKTLA
                     RRFA"
BASE COUNT          334 a          490 c          482 g          281 t
ORIGIN      
        1 atcccaacct acagcttcgc gacagcctca gcaatccgcg aaaccccttc acgaatgcga
       61 tccatggagg ttgcgtagct gatgcggacg taatcatcgt tgccgaacgc gctccccgga
      121 acaacggcca ctcgggcctc atcgagcaga aagttcgcaa atgactgcga gtcggtgatg
      181 ccacggcgcc cgaagaattg cttcacttcg gggaagacgt agaacgcgcc atccggggcc
      241 gagcagcaga agcccgtcac ggcattgagc gccggaacca gccacgctcg ccgctcgcaa
      301 tacgcttcgt acatccgcgt gacggacgat tcgtcgccgc tcagggcctc gatcgccgcc
      361 atctgagcga tggagctcgg gttggacgtg gcgtgactct ggattctccc catcgcacca
      421 atgatctccg ggtgggcaac cgcgtacccc aaccgccacc cggtcatggc gaacgtcttc
      481 gacatcgagt tcaccacgat gatgttctcc ggatagcgat cgaaccagct catggccgac
      541 gcgtgccggt ttccctcgta gacgaatagc tcgtaggtct cgtcgaagat gaggcagagg
      601 tcgcggtcga cgcaccactc gacgatcttc tccaactccg cttcggcgat caccgcccct
      661 gtcggattgc agggggaatt gatgatgacg ccgcgcgtgc gcttgctcga gacgcgctcg
      721 atgtcggcga aggcaggccg aaaatggttc tgcggatcgg cgggggcgaa aaccggcttg
      781 ccacccgcga agagcacctg gtcggggaag ctgacccagt aaggcgaggg gatgatgatc
      841 tcgtcgccat cggtgacgag tgaaagaatg gcgttgaaca gctcctgctt cccgccattg
      901 ccggtgatga cgtgttccat ctgcagcgaa gtgccatagc gccggttgta tcgctcggcg
      961 atcgcatccc gcagcggctt catgccgctc gaattcgtgt acttcgtaaa cccctgttcg
     1021 atggcgcgcc gccccgcgtc ggagatgttt ttcggcgtgc cgaaatccgg ctcacccgga
     1081 ccgaagtcga cgacgtcgac cccgcggctg acgagagcgg tcgctttggt caggacggcc
     1141 agcgtgggcg actcctgaat cgccatcacg cgaggcgtga agcggatttc tcttccggtc
     1201 gggctcacgt cggggacttt caaggagttt tttaccatcg agtcgggagg atcagaaatc
     1261 gggatcaggc aaaccggcgc gccagcgtct tccgcaaccc cgcccacgcc ggcgggacat
     1321 gacgttccca gatgagcatc cggcccatct cgatcaggca ccatgccaga tacacgtaga
     1381 tcatgacgtg ccgcgtggcg ggaaacagga actgggtcag ccagaagacg aacaacacga
     1441 gcgcctcatg aaaacgcagc tcgagattca gcaggaggag gaatccgaga aagctctgca
     1501 ggatggtcag cagaatctcg tgacgctgca tctcgtcgaa aaccagaggg cgcaagtctc
     1561 ccgcgctgat cacgaaaatg atcggga
//
LOCUS       DASWAV010000140         1813 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U2665
            k141_248509, whole genome shotgun sequence.
ACCESSION   DASWAV010000140 DASWAV010000000
VERSION     DASWAV010000140.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960316
            Sequence Read Archive: SRR10260007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 1813)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1813)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:47:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,929
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1813
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_248509"
                     /isolate="SMAG_U2665"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.36444 N 132.31889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1544)
                     /locus_tag="VF958_02790"
     CDS             complement(<1..1544)
                     /locus_tag="VF958_02790"
                     /inference="COORDINATES: protein motif:HMM:NF014759.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-3' exonuclease H3TH domain-containing
                     protein"
                     /protein_id="HEX9148066.1"
                     /translation="MPESPPKLYLVDAMSNIHRAYHAIQRLSTAAGRPTNAIYGFVTM
                     LRKMLREHAPEYLAVAWDGPQRTLRHDAYADYKANRQPMADDLAAQIPAIRQILEAYR
                     IPVLELPGYEADDVIGTLAKKASTMGFDVVIVTADKDMLQLVGPRVRVFHTGREKFLD
                     EAGVREFFGVPPEQVADVLALMGDSVDNIPGVPRVGEVTAKKWIAEYGDLGTLLARAG
                     EIKGKVGESLREHKEDALVSRRLAEIPTDLPIPFEPDALRCTPADAKRLKDLFVELEF
                     HSLAAEIHDESAVRPEISAIRRKPGEAFGVDLSRPLGVALLKAKDRVLLSVSDGENVE
                     IAEEPAAEIVQRWREIDRPGREVATADAKSLDALLAREGASVEADVFDVCLAQYVLSP
                     GAGSSEFEVMAFQRLGHRVTTDKEAGITSCTLPEGYAVETADRWLGERATGTLALAER
                     LRPEMEKKPELEKIYRDIERPLTPVLARMEIAGIRVDIPYLKDMSARMDRDLRALEEK
                     IWAEAG"
BASE COUNT          285 a          620 c          611 g          297 t
ORIGIN      
        1 tcgcccgctt ccgcccagat cttttcctcg agcgcgcgca ggtcccggtc catgcgcgcg
       61 gacatgtctt tcaggtaggg gatgtcgacg cggatgcccg cgatctccat ccgcgcgagc
      121 accggcgtca gcggccgctc gatgtcgcgg tagatcttct cgagctccgg cttcttctcc
      181 atctcgggcc ggagcctctc tgcgagcgcc agcgttcccg tcgcgcgctc gccgagccac
      241 cggtccgcgg tctccaccgc gtagccctcc ggcagcgtgc acgaagtgat tcccgcctct
      301 ttgtcggtcg tcacgcggtg accgagacgc tggaacgcca tgacttcgaa ctccgagctc
      361 cccgcccccg gcgacagcac gtactgcgcg aggcagacgt cgaacacgtc cgcctcgacc
      421 gaagccccct cgcgcgcgag gagagcgtcg agcgacttgg cgtcagccgt cgcgacctcg
      481 cgccccgggc ggtcgatctc gcgccagcgc tgcacgatct ccgcggccgg ctcttcggcg
      541 atctcgacgt tctcgccgtc ggagacggag agcaggaccc ggtccttcgc tttgagcaga
      601 gcgacgccga gcggccgcga caagtcgacg ccgaaggctt cgccgggttt tcggcggatc
      661 gccgagatct ccggacggac cgcggattcg tcgtggatct ccgccgcgag cgagtggaac
      721 tcgagctcga cgaacaggtc cttcagccgc ttcgcgtcgg ccggggtgca ccggagcgcg
      781 tccggctcga acggaatcgg caggtcggtc gggatttccg cgagccgccg cgagacgagc
      841 gcgtcctcct tgtgctcgcg caggctctct ccgaccttgc ccttgatctc gccggcgcgc
      901 gcgaggaggg tccctaggtc gccgtactcg gcgatccatt tcttcgcggt cacctcaccc
      961 acgcgcggca cgcccgggat gttgtccacg ctgtcgccca tgagcgcgag gacgtcggcg
     1021 acctgttccg gagggacgcc gaagaactcg cgcacccccg cctcgtccag gaacttctcg
     1081 cggcctgtgt ggaagacgcg gacccgcggt ccgacgagct ggagcatgtc cttgtcggcc
     1141 gtgacgatga cgacgtcgaa ccccatcgtg gacgctttct tcgccagcgt gccgatcacg
     1201 tcgtccgcct cgtagccggg caactccagg accgggatcc ggtaggcctc caggatctgc
     1261 cggatggcgg ggatctgggc ggcgagatcg tcggccatcg gctggcggtt cgccttgtag
     1321 tcggcgtacg cgtcgtggcg gagggtcctc tgcgggccgt cccacgcgac cgccaggtac
     1381 tccggggcgt gctcccgcag catcttccgg agcatggtca cgaagccgta gatcgcgttc
     1441 gtggggcggc cggcggcggt cgagagccgc tggatcgcgt ggtacgcgcg gtggatgttc
     1501 gacatcgcgt ccaccaggta gagcttcgga ggcgattccg gcatggggag gccgattcta
     1561 gtcggcggtt tccggcgcga cgtcggcgca agctcgagcg ggtgaaaagc gcctgaaatt
     1621 gcgcctggcc ggggaccgag gagcgaggac ggaggacgga cgcgcctcca caccgccacc
     1681 taacgaaaga caccagaagg gttaagagaa aacgttccgg gcggaaaaag aaaacgcgcg
     1741 acccgtttcc ggagtcgcgc gaatcaataa aagaagaata gcaaacgggc acgcgctcgt
     1801 caagcgaaat cct
//
LOCUS       DASWAV010000141         2166 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U2665
            k141_647318, whole genome shotgun sequence.
ACCESSION   DASWAV010000141 DASWAV010000000
VERSION     DASWAV010000141.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960316
            Sequence Read Archive: SRR10260007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2166)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2166)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:47:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,929
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2166
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_647318"
                     /isolate="SMAG_U2665"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.36444 N 132.31889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..259
                     /locus_tag="VF958_02795"
     CDS             <1..259
                     /locus_tag="VF958_02795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011620370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="HIT domain-containing protein"
                     /protein_id="HEX9148067.1"
                     /translation="FEDIAPKAPTHVLVVPRKHIARLADVTAGDEALLGALAARAAAI
                     ANERGLSDFRLVANNGEGAGQSVFHLHFHLLGGRSMRWPPG"
     gene            complement(263..997)
                     /locus_tag="VF958_02800"
     CDS             complement(263..997)
                     /locus_tag="VF958_02800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9148068.1"
                     /translation="MKGILGPILLAVATSLLLAGVVDRIAATVNDEAIPESELRKALV
                     TSALRPEPAESADAFRSRVLEALIDQHLEYEDAQRFGPAPPNAAEIATAMRSLEERLR
                     AEGKDPQDEFVRAGLTREEVRASIERQLLIARYLRERFTPVALADEERAREEYEKFYV
                     PERRAANLAVPPFEAVTDEMRKRAQERAFEEEIEKWRRELRQKARVAIYRIPVPIPTG
                     RQRVELSSIAPVTPAPASTPATTPKP"
     gene            1139..1996
                     /gene="rsmI"
                     /locus_tag="VF958_02805"
     CDS             1139..1996
                     /gene="rsmI"
                     /locus_tag="VF958_02805"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: protein motif:HMM:TIGR00096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0000451 - rRNA 2'-O-methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HEX9148069.1"
                     /translation="MQVSEKAGRLVVVGTPIGNLEDLSPRAVRSLASADAIFCEDTRV
                     TAKLASRLSLSAPRISCPAPRERSRVADLLERLGRGETVALVSDAGMPALSDPGERLV
                     AAAARAGFDVEVVPGPSAPAAALAVSGLPAVPHFFAGFLPARAGERRRALEKMRDREE
                     TLLWFEAPHRLVESLADAAAVLGPRRACVARELTKLHEEAVRATLPELAVIFRSRGAG
                     RGEITVVVEGASARALKGDPASLEAEIRSALERGQSKRGLARELARRSGRPAREIYAR
                     AVEIEAGEQ"
BASE COUNT          307 a          746 c          775 g          338 t
ORIGIN      
        1 gttcgaggac atcgcgccca aagcgcccac gcacgtgctc gtcgtccccc ggaagcacat
       61 cgcgcgcctg gccgacgtca ccgcgggcga cgaagccctg ctgggagctc tcgcggcgcg
      121 ggccgcggcg atcgcgaacg agcgcgggct ctcggacttc cggctcgtcg cgaacaacgg
      181 cgaaggcgcc ggtcagtcgg tcttccacct gcacttccac ctcctcggag gccgctcgat
      241 gagatggccg ccggggtaag actcagggtt tcggagtggt cgccggggtc gaggcggggg
      301 cgggcgtaac cggcgcgatg gaagagagct cgacccgctg tcgcccggtc ggaatcggca
      361 cggggatccg gtagatcgcg acgcgcgctt tctgccggag ctcgcggcgc cacttctcga
      421 tctcttcctc gaaggcgcgc tcctgcgcgc gcttacgcat ctcgtcggtg accgcctcga
      481 acggaggaac ggcgagattc gccgcgcgtc gctccgggac gtagaacttc tcgtactcct
      541 cccgcgcgcg ttcctcgtcg gcgagagcca ccggagtgaa ccgctctcgc aggtatcgtg
      601 cgatgagaag ctgccgctcg atggaagcgc ggacctcctc gcgagtcagt ccggcgcgga
      661 cgaactcgtc ctggggatcc ttcccctcgg cgcgaagtcg ctcttccagg ctgcgcatcg
      721 ccgtcgcgat ctccgcggcg ttcggaggcg ccgggccgaa ccgttgcgcg tcctcgtact
      781 cgaggtgctg atcgatgagc gcctcgagaa cccgcgaacg aaaggcgtcc gccgactccg
      841 ccggctcggg ccgcagggcc gacgtcacca gggccttgcg gagctcgctc tcgggaattg
      901 cctcgtcgtt gaccgtcgcg gcgatgcggt ccaccactcc cgcgagaagg agagaagtcg
      961 ctaccgcgag aaggatcggg ccgaggattc ctttcacgcc ggcatgataa cggcggcccg
     1021 gggtcaggtc tccaattgcc actttgcttc gcaaagatgc caagaccact gcacctccgc
     1081 ggccgctacc tcgatcagtg aagacctgac cccgcccaca ccgtagtatt tgcggacgat
     1141 gcaggtttcg gagaaggcgg gacgtctcgt agtcgtcggc accccgatcg gcaacctcga
     1201 ggacctgtcg ccgcgcgcgg tccggtcgct cgcgtccgcc gatgccatct tctgcgagga
     1261 cacgcgcgtc acggcgaagc tcgcgtcaag gctctcgctc tcggcaccac ggatctcctg
     1321 tccggcgccc cgtgagcgct cgcgcgtcgc ggacctgctc gagaggctcg gccggggtga
     1381 gaccgtggcc ctcgtatcgg acgcgggaat gccggcgctt tcggatccgg gggagcgcct
     1441 cgtggccgcg gcggcgcggg cgggattcga tgtcgaggtc gtgccgggtc cttcggcgcc
     1501 cgcggccgcg ctcgccgtgt cgggactccc ggcggttcct cacttcttcg caggtttcct
     1561 gccggcgagg gcgggcgagc gccgccgggc gctcgagaag atgcgcgacc gcgaggagac
     1621 cctcctctgg ttcgaggcgc cgcatcggct cgtcgagtca ctcgccgacg ccgccgccgt
     1681 gctcgggccg cggcgcgcgt gcgtggcgag agagttgacc aagctccacg aggaggcggt
     1741 ccgagcaacg ctgccggaac tcgcggtgat cttccgctca cgcggcgccg gtcgaggtga
     1801 aatcaccgtc gtcgtcgagg gcgcgtccgc ccgggcgctt aaaggcgacc cggcttccct
     1861 cgaggcggag atccgcagcg cgttggagcg cggccagtcg aagcgcggcc tcgcgcggga
     1921 gctcgcgcgg aggagcggcc ggccggcgcg ggagatctat gcgcgggccg tcgagatcga
     1981 ggcaggggag caatgagcga gcggggtgag ccggtcgtgc gcgtcgccgg cctgacgaag
     2041 gttttccgga cttatcgccg cgaggaggga ctcgccgccg cgctgaagtc gctcgtgcac
     2101 cgggtcccga ccgagatcgc cgcggtctcg gacgtcacgt tctcgatcga gccgggcgaa
     2161 atggtc
//
LOCUS       DASWAV010000142         2923 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U2665
            k141_208566, whole genome shotgun sequence.
ACCESSION   DASWAV010000142 DASWAV010000000
VERSION     DASWAV010000142.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960316
            Sequence Read Archive: SRR10260007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2923)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2923)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:47:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,929
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2923
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_208566"
                     /isolate="SMAG_U2665"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.36444 N 132.31889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            33..>2923
                     /locus_tag="VF958_02810"
     CDS             33..>2923
                     /locus_tag="VF958_02810"
                     /inference="COORDINATES: protein motif:HMM:NF017930.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9148070.1"
                     /translation="MQATELLLQERMPRGVAVARPRAEEVQTHLHVRDYVLPALRRFT
                     SPHDVIPRTHVLSNGRYSVMVTAAGSGYSRWLGRDVTRWREDVTLDACGTWMYLRDVQ
                     SGLVWSAGYQPSGVEPSSYEVVYSEDRAEIRRRDGALATTLEIVVSAEDDAEVRQVSV
                     TNLGTRPRTIEITSYAEVVLATPASDEAHPVFSNLFVETEFVPRLEALLATRRPRSSD
                     EERLWLAHVSTVEGNEVGTIQFETDRARFLGRGRQVRSAGSIHDGRPLSNTVGNVLDP
                     IVSLRRRLRVEPGSTGRVTFTTVAAGDRDAVLRLADKYRDPAAFERTTTLAWTQAQVQ
                     LRHLNVDADEAHLFQRIANRVLYSDPSLRASSEVIARNRLGKSALWRHGISGDLPIVL
                     VRIDFAEDREIVRQLLRAHEYWIMKGLSVDLVIINEQGTSYLQDLQGALEALVRASER
                     PARRDTEPVRGGIFLLRGDLLTDADRDFLRTVARAVLLSRHGTLAEQVVRHLRVEPAP
                     EPPRALKPAPPTVDITPPRPPLEFFNGLGGFAEDGREYVTILGPGQATPAPWVNVIAN
                     DRFGFLVSESGSGFTWAGNSRENQLTPWSNDPVADPAGEALFVRDEATGEVWSPTPLP
                     VREDWPYVARHGQGYSRFEHESHGIALDLLQYVAKDDPIKISRLRIENRSATPRRLSV
                     TAYVEWVLGSSRSRSAPFVVTDLDTATGAIFARNSWNEEFGEHVAFADLGGRQSAWTA
                     DRTEFLGRNGTLERPAALSRGVKLGKHMGGGLDPCAALQTTFELSPGDRTEIAFLLGE
                     GENEDQARNLVKRYREADLEAVLNEVRAQWDDVAGALQVKTPDRSMDILLNRWLLYQA
                     LSCRVRARAAFYQAGGAYGFRDQLQDVIALLTSKRELAREHLLRAAAQQFVEGDVQHW
                     WHPPSGRGVRTRISDDRLWLPYAVGRYLAVTDDTAILDEAVPYLDGPALL"
BASE COUNT          445 a         1031 c          992 g          455 t
ORIGIN      
        1 tcatgcggaa acgtttccac gaagagccga gggtgcaggc gaccgagctt ctcctgcagg
       61 agcgcatgcc gcgcggagtg gcggtcgcgc gtccgcgggc cgaggaggtc cagacccacc
      121 tccacgtgcg cgactacgtc ctgcccgccc tgcggcgatt cacctcgccg cacgacgtga
      181 tcccgcggac ccacgtcctt tcgaatggcc gctactcggt gatggtgacc gcggcgggct
      241 cgggctacag ccgctggctc gggcgcgacg tcacgcgctg gcgtgaagac gtcactctcg
      301 acgcctgcgg aacgtggatg tacctgcgcg acgtgcagag cgggctcgtg tggtcggcgg
      361 gctatcagcc gagcggcgtc gagccgagca gctacgaagt tgtctactcg gaggatcgcg
      421 cggagatccg gcggcgcgac ggggcgctcg cgacgactct cgagatcgtc gtttccgccg
      481 aggacgacgc ggaagtccgc caggtgtcgg tgacgaacct cggaacccgg ccccgaacga
      541 tcgagatcac gtcatacgcg gaggtcgtgc tggcgacgcc cgcctcggac gaggcccatc
      601 ccgtcttttc caacctcttc gtcgagacgg agttcgtccc ccggctcgaa gcccttctcg
      661 cgacgcgtcg cccgcgctcg tccgacgagg agcgcctctg gctcgcccac gtctcgaccg
      721 tcgagggaaa cgaggtcggt acgatccagt tcgagacgga ccgcgcccga ttcctggggc
      781 ggggccgcca ggtccgcagc gccggctcga tccacgacgg gcggccgctc tcgaataccg
      841 tcgggaacgt cctcgatccg atcgtttctc tgcggcgccg gctccgcgtc gagccgggat
      901 cgaccggacg cgtcactttt acgactgtcg cggccggcga ccgcgatgcc gtcctccgac
      961 tcgccgacaa gtaccgtgat ccggcggcgt tcgagcgcac gacgacgctc gcctggacgc
     1021 aagcgcaggt ccagctgcgg cacctgaacg tggacgcgga cgaagcgcac ctcttccagc
     1081 ggatcgcgaa tcgcgttctc tattccgacc cctccctgcg cgcttcatcg gaggtcatcg
     1141 cgcgcaaccg gctcgggaag tccgctctct ggcgccacgg catctccggg gacctgccga
     1201 tcgtgctcgt ccggatcgac ttcgccgagg accgcgagat cgtccgccag ctcctgcgcg
     1261 cgcacgagta ctggatcatg aaagggctgt cggtcgacct cgtcatcatc aacgagcagg
     1321 gcacgtctta cctccaggac ctgcagggcg cgctcgaggc actcgtccgc gcgagcgagc
     1381 ggccggcgcg gcgcgatacg gagcccgtgc gaggcggcat attcctcctg cgcggagatc
     1441 tgctgacaga cgcggatcgc gatttcctcc gcaccgtggc gcgcgccgtc ctgctctcac
     1501 gtcatgggac tctcgccgag caggtcgtgc ggcatctgcg cgtggaaccc gcccccgagc
     1561 ctccgcgcgc gctcaagccc gcgccgccga cggtggacat cactcctccg cgcccgcctc
     1621 tcgagttctt caatggcctc ggcggcttcg ccgaagacgg ccgggagtac gtgacgatcc
     1681 tcggacccgg acaggcgacg ccggcccctt gggtcaacgt catcgcgaac gatcgtttcg
     1741 gcttcctggt gtcggaatcc gggtccgggt ttacgtgggc gggcaacagc cgggagaacc
     1801 agctgacgcc ctggtcgaac gacccggttg ccgacccggc gggcgaggcg ctgttcgttc
     1861 gcgacgaagc gaccggggaa gtgtggtcgc cgacgccgct ccccgtccgg gaagactggc
     1921 cctacgtcgc ccgccacggg cagggctaca gccggttcga gcacgagagc cacggcatcg
     1981 ctctcgacct cctgcagtac gtcgcgaagg acgacccgat caagatctcg cgccttcgca
     2041 tcgagaaccg gtcggcgacg ccgcggcggc tttcggtcac cgcgtacgtg gagtgggtgc
     2101 tcggatcgtc ccggagccgc tctgcgccgt tcgtcgtcac ggacctcgac accgccaccg
     2161 gcgcgatctt cgcgcgcaac tcgtggaacg aggagttcgg ggagcacgtc gccttcgcgg
     2221 acctcggcgg ccggcagagc gcatggaccg ccgatcggac ggagttcctc ggccggaacg
     2281 gcaccctcga gcggccggct gccctgtcgc gaggagtgaa gctgggaaag cacatgggcg
     2341 gggggctcga tccctgcgcg gctctccaga cgaccttcga attgtccccc ggcgaccgca
     2401 cggaaatcgc gttcctgttg ggagaaggcg agaacgaaga tcaggcccgg aacctcgtga
     2461 agcgataccg ggaagcggat ctcgaagcgg tcctcaacga agttcgcgcc cagtgggacg
     2521 acgtcgccgg cgcgctgcag gtgaagacgc cggaccggtc gatggacatt ctcctgaacc
     2581 gctggctgct ctatcaggcg ctctcgtgcc gcgtgcgggc gcgggccgcc ttctaccagg
     2641 cgggcggcgc gtacgggttc cgcgaccagc tccaggacgt cattgccctg ctgacctcga
     2701 agcgcgagct cgcgcgcgaa cacctcctga gggccgcagc gcaacagttc gtggaggggg
     2761 acgtccagca ctggtggcat ccgccgtcgg gtcggggcgt ccggacccgg atctcggacg
     2821 atcgcctctg gctgccgtac gccgtcggtc gctacctggc ggtcacggac gacacggcga
     2881 tcctcgacga ggccgtcccc tacctcgacg ggcccgctct gct
//
LOCUS       DASWAV010000143         3160 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U2665
            k141_168777, whole genome shotgun sequence.
ACCESSION   DASWAV010000143 DASWAV010000000
VERSION     DASWAV010000143.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960316
            Sequence Read Archive: SRR10260007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 3160)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3160)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:47:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,929
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3160
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_168777"
                     /isolate="SMAG_U2665"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.36444 N 132.31889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..276
                     /locus_tag="VF958_02815"
     CDS             <1..276
                     /locus_tag="VF958_02815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HEX9148071.1"
                     /translation="ELKSVQNYMAGVFVLQNSSRSGIINVLELIDLHGLPADYLKTYV
                     QRVYAVTPQDVQRLTQKYIQDDKATIVVVGDRKVIEEQIKPYGTIAP"
     gene            325..1824
                     /locus_tag="VF958_02820"
     CDS             325..1824
                     /locus_tag="VF958_02820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013228418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS1 family nucleobase:cation symporter-1"
                     /protein_id="HEX9148072.1"
                     /translation="MAASRANEIRHPDGRVELADTSRIDASPLSNPDLAPVPIRRRNW
                     TTYNYAALWISMAHCIPTYMLASGLMASGMNWRQALVTILLGNVIVLIPILLNSHPGT
                     KYGIPFPVFARAAYGTLGSNLPALMRALVACGWFGIQSWIGGEALHTFFTAIIPGWPT
                     LLGSGFGGHPTTAWLSFLLFWGINIFIIYRGMDLLRKVENWAAPFVLVMTAILLWWAV
                     GKANGLGPLLAQPGKFTTFREFFPVFIPSLTAMIGYWATLSLNMPDFTRFGKSQREQA
                     VGQVVALPTTMTIFAAMGVLITSATVIIYGRAIWDPVQLVGQFRAPVVIAISMFTAVV
                     ATLAVNIAANVVSPANDFANAFPRLIRFKTGGLITGIIGILMQPWKLLADPSGYIFLW
                     LLGYSGGLGSIAGVLIADYWLVRKKELDLENLYLPGGIYRYSGGWNWKAVVATLAGCA
                     LAWGGLVVPALKPLYSYAWFVGFFSSALLYWALSARATAAVAEALARAD"
     gene            1862..2599
                     /locus_tag="VF958_02825"
     CDS             1862..2599
                     /locus_tag="VF958_02825"
                     /inference="COORDINATES: protein motif:HMM:TIGR00530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HEX9148073.1"
                     /translation="MKLVGEKLAYYAGFLVGGLWFLFCSALGIVWLLVRPHNRQTLYV
                     YGRVFCRGVVSLMGWRLDVANRELLDSARPCVFVANHQSFLDVVTFGSMFPKRTVSAG
                     KREIGRIPVFGWFYRLSGNLVIDRGNPLAARDSLDDAARTIRDERVSVWFMPEGHRNA
                     GPELLPFKTGAFRLALAAGVPIVPIVAAPLSAIADTKRRLARPGTLQVTVLDPITTDA
                     SSPRAVAELTELARTAMQEELDRLSRS"
     gene            2645..2794
                     /locus_tag="VF958_02830"
     CDS             2645..2794
                     /locus_tag="VF958_02830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9148074.1"
                     /translation="MVVFLILALFGLAYTAWRLVRNRRNPDAWEGGLLWLMSFLVVAI
                     LLART"
     gene            complement(2862..>3160)
                     /locus_tag="VF958_02835"
     CDS             complement(2862..>3160)
                     /locus_tag="VF958_02835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9148075.1"
                     /translation="LVGGAIRDFPTAGVCGVPFSAKAVAVNLTVVLPTTDGYLTLYPA
                     GTFLPLASTINFRTGIVRANNAVIPLGTAGQVSVFCGMPSGQTNFLLDVTGWFE"
BASE COUNT          491 a         1065 c         1002 g          602 t
ORIGIN      
        1 gagttgaagt cggtccagaa ctacatggcc ggcgtcttcg tgctgcagaa ctcgtcgcgc
       61 agcgggatca tcaacgtcct cgagctgatc gaccttcacg ggctacccgc ggactacctg
      121 aagacctacg ttcagcgcgt gtacgccgtc acgccccagg acgtccagcg gctcacgcag
      181 aagtacatcc aggacgacaa ggcgacgatc gtggtcgtcg gggacaggaa ggtcatcgag
      241 gagcagatca agccctacgg gacgatcgcc ccgtaacgcc ggctcccgta tcatcatccc
      301 aacggattcc gaggagggtt gatgatggcg gcctcgcggg ccaacgaaat ccgtcacccc
      361 gacgggaggg tcgagctcgc cgatacgtcg cgcatcgacg cgagcccgct ctccaacccc
      421 gacctcgctc cggtcccgat ccgccggcgc aactggacca cgtacaacta tgccgcgctc
      481 tggatcagca tggcccactg catccccacg tacatgctcg cgtcgggcct gatggcgagc
      541 gggatgaact ggcgccaggc gctcgtcacg attctgctcg gaaacgtgat cgtcctcata
      601 ccgatcctgt tgaactctca cccggggacg aagtacggga tcccgttccc cgtcttcgcc
      661 cgggccgcct acgggacgct cggatcgaac ctcccggcgc tcatgcgcgc gctcgtcgcc
      721 tgcggctggt tcggcatcca gtcgtggatc gggggggaag cgctccacac gttcttcacg
      781 gcgatcattc ccggctggcc gacgctcctc ggaagcggct tcggaggcca cccgacgacg
      841 gcgtggctct ccttccttct cttctgggga atcaacatct tcatcatcta ccgcggcatg
      901 gacctcctgc ggaaggtgga gaactgggcg gcgccgttcg ttctcgtgat gaccgcgatc
      961 ctcctctggt gggcggtggg caaggcgaac ggcctcggac ctctgctcgc gcagcccggg
     1021 aagttcacga cgttccgcga gttcttcccc gtgttcattc cctcgctgac cgcgatgatc
     1081 ggctactggg cgacgctgtc gctcaatatg cccgacttca cgcgcttcgg aaagagtcag
     1141 cgcgagcagg ccgtcggaca ggtggtcgcc cttccgacga cgatgacgat attcgccgcg
     1201 atgggcgtcc ttatcacgag cgcgacggta atcatctacg ggcgagcgat ctgggatccg
     1261 gtgcagctcg tcgggcagtt ccgcgccccg gtcgtcatcg cgatctcgat gttcaccgcg
     1321 gtcgtcgcga ctctggccgt gaatatcgcg gcgaatgtcg tctcgccggc caacgacttc
     1381 gcgaacgcgt ttccgcgtct cattcgcttc aagaccgggg gcctgatcac cgggatcatc
     1441 ggcatcctca tgcagccctg gaagcttctc gccgacccct ccggatacat ctttctgtgg
     1501 ctcctcggct actccggggg gctcggatcc atcgccggcg tgctcatcgc ggactactgg
     1561 ctcgtccgga agaaggagct cgacctcgag aacctctatc tccccggcgg catctaccga
     1621 tattccggag gctggaactg gaaggcggtg gtcgcgacgc tcgccgggtg cgcgctcgcg
     1681 tggggcggtc tcgtcgtgcc ggcgttgaag ccgctctact cgtacgcgtg gttcgtcggg
     1741 ttcttctcct cggccctgct ctactgggcg ctgtcggcgc gggcgaccgc ggcggtcgcc
     1801 gaagctctgg cccgggccga ctgacctcgc gtctggcgac tgccgccgat aatctgccgc
     1861 cgtgaagctc gtcggggaga agctcgccta ctacgccgga ttcctcgtcg gcggcctctg
     1921 gttcctcttc tgctccgcgc tcgggatcgt ctggctcctc gtgcggccgc acaatcgcca
     1981 aactctctac gtctacggca gggttttctg ccgcggagtt gtgtcgctca tggggtggcg
     2041 actcgacgtc gcgaaccgcg agcttctcga ctctgcgcgt ccgtgcgtct tcgtcgcaaa
     2101 ccaccagagc ttcctggacg tcgtcacgtt cggctccatg tttccgaagc ggacggtctc
     2161 ggcgggaaag cgcgagatcg gccggatccc ggtcttcggg tggttttacc gcttgtcggg
     2221 gaacctggtg atcgatcggg gtaacccgct cgccgctcgc gattcgctcg acgacgcggc
     2281 gcgaacgatc cgcgacgagc gcgtgtcggt ctggttcatg ccggagggac accgaaacgc
     2341 cggcccggag ctcctgccgt tcaagaccgg cgcgttccgg ctcgcgcttg ccgccggagt
     2401 gccgatcgtg ccgatcgtgg ccgcgccgct ctccgcgatc gcggatacga aacgccggct
     2461 tgcgcggccg ggaacgctcc aggtcacagt gctcgatccg atcacgacgg acgcgagctc
     2521 tccccgggca gtggcagagc tcacagagct cgcgaggacg gcgatgcagg aggaactgga
     2581 ccggctgagc cgttcctgag gacttcccgg cgcgctggca gaccttttgc tcctttcgcg
     2641 ctcgatggtc gtcttcctga tcctagcgct cttcggcctc gcgtacaccg cgtggcggct
     2701 cgttaggaac cggcggaatc ccgacgcctg ggagggaggg ctgctctggc tcatgtcctt
     2761 cctcgtcgta gcgatcctgc tcgcgcggac gtagcgagtc ggtctcagtt ctcagttgtc
     2821 agttctcagc cgaggactga cggctgagca ctgcgaattt cctactcgaa ccagcccgtg
     2881 acgtcgagga ggaagttcgt ctgtcccgac ggcatcccgc agaaaaccga cacctgtccg
     2941 gccgtgccga gaggaatgac ggcgttgttc gcgcgcacga ttccggtccg gaagttgatc
     3001 gtgctcgcga gcgggagaaa agtacccgcc ggatagagcg tcaggtatcc atcggtcgtt
     3061 ggcagtacca cggtcaggtt gacggcgact gccttcgccg agaacggcac gccgcagacc
     3121 cccgccgtcg gaaagtcccg gatcgccccc ccgacgagcg
//
LOCUS       DASWAV010000144         2733 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U2665
            k141_527434, whole genome shotgun sequence.
ACCESSION   DASWAV010000144 DASWAV010000000
VERSION     DASWAV010000144.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960316
            Sequence Read Archive: SRR10260007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2733)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2733)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:47:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,929
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2733
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_527434"
                     /isolate="SMAG_U2665"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.36444 N 132.31889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..835
                     /locus_tag="VF958_02840"
     CDS             <1..835
                     /locus_tag="VF958_02840"
                     /inference="COORDINATES: protein motif:HMM:NF027890.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HEX9148076.1"
                     /translation="RREDDSGRPVRLLAIAEEQSLALSFSRSGRLISPVPDEGETVKK
                     GNTVARIEEPGLSEDATDFERQVAETRAREKSRQEDVARLQAQLAEATSEEKRVARLV
                     KEGIAPSAQLETVRHRREGIAAEIRGREAEKGQIDAEGEALQVRLGKVRRFEKEGALI
                     APASGTVLTRHHRQGEWVTAGDPIVTLQLAAPYLRVEVPEEKLSAFAVGRTVAVWPQA
                     RPDSRTRARVVSIRPRSEFATRRNWGLQSRDLRTFSVRLAADDLPVVSGQTFIVEAGA
                     N"
     gene            900..1787
                     /locus_tag="VF958_02845"
     CDS             900..1787
                     /locus_tag="VF958_02845"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX9148077.1"
                     /translation="MTFAVRPGETYGLLGPNGAGKTTLVRMLTTLLRPTSGSARVAGY
                     DVVREKGMVRASIGTVSQAETTDENLTVRENLSVQGKMYGMWGRRLSEMIERRLTQVG
                     LWEKRGQITKTLSGGMRRRLEIARGVLHSPHILFLDEPTTGLDPQSRRAVWDMLGELK
                     RIDGSLAIVLTTHAMEEANFLCDRVAIMDRGHILCEDAPETLKRSLPTGERVELETDR
                     PIEPRDRDSILALGAVNWRTPAPTAAQFEARPGQGVGRLAAELVEERGYRILHLTIAP
                     VTLEDVFFAYTGRALRESA"
     gene            1795..>2733
                     /locus_tag="VF958_02850"
     CDS             1795..>2733
                     /locus_tag="VF958_02850"
                     /inference="COORDINATES: protein motif:HMM:NF024110.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX9148078.1"
                     /translation="MSGRAEAAQAPGMAGERVRGISRGPRLALLGILAICQRAYLTLW
                     RQPVLIISTMLFPLIYLAVLGNALNRQLVNVPIAVVDEAGNSLAAECRRGVLALETGR
                     NLVVAEFPADREEALRDLRRGRYRSVWVLPYGLSAKGPTPAFIGDNTDRFSYETVEAA
                     LADIWRDATGVRRTSAEPVRLEAYPYLDYLTYLGPAVVCLAIFMGSMVAGGLQVIEDR
                     MYGYHEGYLVTPVSTGAIVTGHMLAGTLVASLAGALVLVVIMLFTPLTMATPEALGGG
                     LVTVFLTSLAISALWFLLFARARRTSVLRGMFGIINV"
BASE COUNT          426 a          936 c          954 g          417 t
ORIGIN      
        1 gcgccgggaa gacgactcgg ggcgccctgt ccggcttctg gccatcgcgg aggaacagag
       61 ccttgctctt tccttctctc gcagcggacg cctgatcagt cccgtgccgg acgagggcga
      121 gaccgtcaag aaaggcaaca ccgtcgcccg catcgaggag cccgggctct ccgaggacgc
      181 gacggacttc gagcgccagg tggcggagac gcgggcgcgg gagaagagcc gccaggagga
      241 tgtcgcgcgc ctgcaggcgc agctcgccga ggccacgagc gaggagaagc gcgtcgcacg
      301 gctcgtgaaa gaggggatcg ctccgtccgc gcaactggaa acggtgcggc accgccgaga
      361 ggggatcgcc gccgagatcc gaggccgaga ggcggagaag ggtcagatcg acgcggaggg
      421 cgaggccctc caggtgcgcc tcggcaaggt gcgccggttc gagaaggaag gcgctctcat
      481 tgcgccggcg tcgggaacgg tgctgacccg tcatcaccgg cagggagagt gggtgacggc
      541 gggcgatccg atcgtcaccc tccagctcgc ggcgccgtac ctgcgagtcg aggttcccga
      601 ggagaaactc tcggcgttcg cggtcggcag gacggtggcc gtgtggcctc aggcgcggcc
      661 cgactcgcgc acgcgcgccc gcgtcgtgtc gatccggccg aggtcggagt tcgcgacgcg
      721 gcgcaactgg ggcctgcaga gccgcgacct ccgcacgttc tcggtccgcc tcgctgcgga
      781 cgatcttccc gtcgtctcgg gccagacgtt catcgtcgag gcgggcgcga attagcgcaa
      841 actcgatcga ggccgagagc ctcaccaaga gattcgacgg cgtcgcagtc gacgacggca
      901 tcacgttcgc cgttcgaccc ggcgagacct acggcctcct cgggcccaac ggggccggca
      961 agacgacgct cgttcgcatg ttgacgacac tcctccgacc cacgtccggg tcggctcgcg
     1021 tcgccggcta cgacgtcgtc cgcgagaagg gaatggtccg cgcctccatc ggcacggtct
     1081 ctcaggccga gacgaccgac gagaacctca ccgtgcgcga gaacctgtcg gtgcagggaa
     1141 agatgtacgg catgtggggg cggcgactct ccgagatgat cgagcggcgg ctcacgcagg
     1201 tcggtctctg ggagaagcgc ggccagatca ccaagaccct ctcggggggg atgcgccggc
     1261 gcctcgagat cgcgcgcggc gtcctccact cgccccacat cctgttcctc gacgagccaa
     1321 ccaccgggct cgacccccag agccgtcgcg ccgtctggga catgctcggt gagttgaaga
     1381 gaatcgacgg aagcctcgcg atcgtgctca cgacgcacgc gatggaagag gcgaatttcc
     1441 tctgcgatcg tgtcgcgatc atggatcgcg gccacatcct ctgcgaggac gcgcccgaga
     1501 cgttgaagcg cagccttccg accggcgagc gcgtggagct cgagaccgac cggcccatcg
     1561 agccacgcga ccgggactcg atcctcgcgc tcggcgccgt caactggcgc acgcccgctc
     1621 cgaccgctgc acagttcgag gcccgtccgg gccagggcgt gggccggctc gccgcggaac
     1681 tcgtggagga gcggggctat cgaattcttc acctgacgat cgcgccggtg acgctcgagg
     1741 acgttttctt cgcgtacacc ggacgggcgt tgcgggagag cgcgtgaggc ggagatgagc
     1801 ggccgcgcgg aggcggcgca ggcgccgggc atggcggggg aaagggtgcg cggcatctcc
     1861 cgcgggccgc gcctcgctct cctcggcatc ctcgcgatct gccagcgcgc atacctgacg
     1921 ctctggcgac agcccgtcct gatcatctcg acgatgctct tcccgctcat ttacctcgcg
     1981 gtgctcggca acgcgttgaa tcgccagctc gtcaacgtgc cgatcgccgt cgtggacgaa
     2041 gcgggcaact cgctcgcggc tgagtgccgc cgcggcgtac tcgcgctcga gacgggccgc
     2101 aacctcgtcg tcgcggagtt ccccgcggac cgcgaggaag ccctgcgcga cctgaggcgg
     2161 ggccgctatc gcagcgtgtg ggtgttgccg tacgggctgt cggcaaaggg accgactccg
     2221 gctttcatcg gcgacaacac ggaccgcttc tcgtacgaga ccgtcgaggc cgcgctcgcc
     2281 gacatctgga gagacgcgac cggcgtacgc cgcacgtctg cggagcccgt gcgtctcgag
     2341 gcgtatccgt atctcgacta cctgacctat ctcggacccg cggtcgtgtg cctcgcgatc
     2401 ttcatgggct ccatggtggc cggcggcctg caggtgatcg aggaccggat gtacggatat
     2461 cacgagggct atctcgtgac ccccgtctcg accggcgcga tcgtgaccgg ccacatgctc
     2521 gccgggacgc tcgtcgcgag cctcgccggc gcgctcgttc tcgtcgtcat catgctgttc
     2581 acgccgctga cgatggcgac gcccgaggcg ctcggcggcg ggctcgtcac cgtgttcctg
     2641 acgtcgctcg ccatctccgc gctctggttc ctactcttcg cccgtgcccg caggacgagc
     2701 gtgctccgcg gcatgttcgg gatcatcaac gtc
//
LOCUS       DASWAV010000145         2033 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U2665
            k141_766392, whole genome shotgun sequence.
ACCESSION   DASWAV010000145 DASWAV010000000
VERSION     DASWAV010000145.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960316
            Sequence Read Archive: SRR10260007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2033)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2033)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:47:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,929
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2033
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_766392"
                     /isolate="SMAG_U2665"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.36444 N 132.31889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1373
                     /locus_tag="VF958_02855"
     CDS             <1..1373
                     /locus_tag="VF958_02855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9148079.1"
                     /translation="IYLSEGFESRLLVGTRDTDQERDWILSGEQWKVDAEKRFGNSHL
                     QQQVSFMTDLFRRSDCVIHAVDIAGLATDIDPSSQSSPVRGENALFEIANATGGEVLR
                     NDNDFRAQLDRLVARTSLVYVLAFRPEKTGKDDRFHDLKVKVRVPGARVSSRAGYYEK
                     RPFHVLSPLERSLSAADIIANEIPISDIPTRVLAIPFPRQEGSDVRAAVPVLVEIAGD
                     RLLSGVIAEHADAEIYVYASDSENRLQDFFAQVIGIDLSRNREKLEKGGLRYAGQLSL
                     PPGDYRLRVLVRNSQTGRAGLAVQSLHVPTFTAGEPYLVPPIFLEPANGGMLVRRSGS
                     SGASSEDPLLGAGVEDFVPTAAPAVLLGSPSRVSVVTYHFGAENREPILKMGAQVLTE
                     DGRPLGDGLIALVGKGPVEADGRQLLLLSFTPAKLSPGRYALRVIVQDGATGRGTHAS
                     APFLVP"
     gene            1374..>2033
                     /locus_tag="VF958_02860"
     CDS             1374..>2033
                     /locus_tag="VF958_02860"
                     /inference="COORDINATES: protein motif:HMM:NF025910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX9148080.1"
                     /translation="MIPAVVLLLAGTARPAPTAAPADPLHARVQTLRRRSEGVRPGTP
                     EARSIAGELGQIGRAYLERGDAGRAVELLEEAYGWDEENGLVLALLTLAYVRRGDFPF
                     ARFYLDLAEQRAPRAPPQAYEVLGEVYYSLNRLEDAVAAWEHFQRIGGESPATLRQLS
                     KLRQELALASGQRHFENERFSFYWDPAISAPVVEEIADRLAAMYDEQSAFFGTDLTTA
                     QI"
BASE COUNT          322 a          699 c          670 g          342 t
ORIGIN      
        1 tgatctatct ctccgagggc ttcgagagcc ggctgctcgt cgggacgcgg gacaccgacc
       61 aggagcgcga ctggattttg agcggcgagc agtggaaagt ggatgcggag aagaggttcg
      121 gcaactccca tctccagcag caggtcagtt tcatgaccga cctcttccgc cgctcggact
      181 gcgtgatcca cgcggtcgac atcgcggggc tcgcaaccga catcgacccc agctctcaga
      241 gctccccggt gcgcggcgaa aacgcgctct tcgagatcgc gaatgccacc gggggagagg
      301 tcctgcgcaa cgacaacgac ttccgcgcgc aactcgatcg tctcgtcgcc cggacgagcc
      361 tcgtctatgt actggcgttt cggccggaga agaccggcaa ggacgaccgg ttccacgacc
      421 tgaaagtgaa agtgcgggtc ccgggcgccc gggtgtcgtc gcgggcgggc tactacgaga
      481 agcggccctt ccacgttctt tcgcccctcg agcgcagcct gtcggctgcg gacatcatcg
      541 cgaacgagat cccgatctcc gacatcccga cgcgcgtgct cgccattccg tttccgcgcc
      601 aggagggaag cgacgtccgc gcggcggttc ccgttctcgt cgagattgcc ggcgatcggc
      661 tgctctccgg cgtcatcgcg gaacacgcag acgcggagat ctacgtgtac gcgagcgatt
      721 ccgagaaccg gctgcaggac ttcttcgcgc aggtcatcgg catcgatctg tcgcgcaatc
      781 gcgagaagct cgagaagggc gggctgcgtt atgccggcca gctctccctc ccgcccggcg
      841 attacaggct ccgggtgctc gtgcgcaact cccagaccgg acgcgccggc ctcgccgttc
      901 agtcgctcca cgtcccgacg ttcaccgccg gcgagccata cctcgttccg ccgatcttcc
      961 tcgagcccgc caacggcggc atgctcgtgc gtcggagcgg atcgtcggga gcctcttcgg
     1021 aagacccgct gctcggagcg ggcgtggagg acttcgtccc cacggccgcc ccggcagttc
     1081 tcctcggcag tccgtcgcgc gtgtcggtcg tcacgtatca cttcggcgcc gagaaccgcg
     1141 agccaatttt gaagatgggc gcgcaggtcc tgacggagga cggccgcccg ctaggcgacg
     1201 gcctgatcgc actcgtggga aagggtccgg tcgaagcaga cggccgccag ctcctcctcc
     1261 tttccttcac gcccgcaaag ctctcccccg gacggtatgc gctgcgcgtg atcgtgcagg
     1321 atggagcgac cgggcggggg acccacgctt cggcgccgtt cctcgttccc tgagtgattc
     1381 cggcagtcgt ccttctcctc gccggaaccg cgaggccggc tccgaccgcc gcgccggccg
     1441 acccccttca cgcgcgggtg cagacgctcc ggcggcgatc cgagggggtc cggcccggaa
     1501 ccccggaagc gcgatcgatc gcgggcgagc tcggtcagat cgggcgcgcc tatctcgagc
     1561 ggggagacgc cgggagggcc gtggagcttc tcgaggaagc gtacggctgg gacgaggaaa
     1621 acgggctcgt gctcgcgctg ctcacgctcg cgtacgtccg gcgcggcgat tttccgttcg
     1681 cgcgcttcta tcttgatctc gccgagcaac gcgccccccg cgcacctccc caggcgtatg
     1741 aagtcctggg agaggtctac tactctctga atcgtctcga ggatgccgtc gctgcctggg
     1801 agcatttcca gaggatcggc ggcgaaagcc cggcgacctt gcggcaactc tcgaaactgc
     1861 ggcaggagct cgctctcgcg tccgggcagc ggcacttcga gaacgagagg ttctccttct
     1921 actgggaccc ggcgatctcc gcgccggtgg tcgaagaaat cgccgaccgg ctggcggcga
     1981 tgtacgacga gcagtcggcg ttcttcggga cggatctcac aacggcgcag atc
//
LOCUS       DASWAV010000146         1689 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U2665
            k141_248717, whole genome shotgun sequence.
ACCESSION   DASWAV010000146 DASWAV010000000
VERSION     DASWAV010000146.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960316
            Sequence Read Archive: SRR10260007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 1689)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1689)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:47:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,929
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1689
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_248717"
                     /isolate="SMAG_U2665"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.36444 N 132.31889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(40..312)
                     /locus_tag="VF958_02865"
     CDS             complement(40..312)
                     /locus_tag="VF958_02865"
                     /inference="COORDINATES: protein motif:HMM:NF015589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 65 protein"
                     /protein_id="HEX9148081.1"
                     /translation="MYRAGIEWILGFRFRGAVLHVDPCIPRWWPGYHITFRYHSARYE
                     LVVENPHGANCGVARIVADGRELPGEIREIPLVDDGKAHRIEVLLG"
     gene            complement(292..1431)
                     /locus_tag="VF958_02870"
                     /pseudo
     CDS             complement(292..1431)
                     /locus_tag="VF958_02870"
                     /inference="COORDINATES: protein motif:HMM:NF028476.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphorylase"
     gene            complement(1447..>1689)
                     /locus_tag="VF958_02875"
     CDS             complement(1447..>1689)
                     /locus_tag="VF958_02875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase 36 associated protein"
                     /protein_id="HEX9148082.1"
                     /translation="ITLAPGERAEVRVFLGEARDEAEARRLVTAHREKDADATLRQIR
                     RFWDGVLGGLQIKTPDPALDVFVNRWLLYQALSCRL"
BASE COUNT          269 a          616 c          551 g          253 t
ORIGIN      
        1 cagcaagcgg agagagtgta aggcagtccc gacgctgacc taccccaaca gcacttcgat
       61 gcgatgggcc tttccgtcgt cgacgagcgg aatttcccgg atttcgcccg gcagctcgcg
      121 gccgtccgcg acgatccgag cgacgccgca gttcgcgccg tgcgggttct cgacgacgag
      181 ctcgtatcgc gcggagtgat accggaacgt gatgtggtaa ccgggccacc accgcgggat
      241 gcacgggtcg acgtggagca cggcgccccg aaagcggaac ccgaggatcc actcgatgcc
      301 cgcgcggtac atccagcctg cggatcccgt gtaccacgtc caaccgccgc gcccgacgtg
      361 gggccgctcg gcgtagacgt ccgccgccgc cacgtacggc tcgaccttgt agcgatagac
      421 gccggcgcgg gtgccggcgt ggttgatcgg gttcaagatc gagaagagct ctccggtctt
      481 gtccccctgg cgcagccccg agaacgcgat cagcgaccac agcgcgccgt gcgtgtactg
      541 accgccgttc tcgcgcacgc cgggcagata tcccttgatg tatccggggt cgagcggagt
      601 cttgtcgaac ggcggtgtga acaagaggac gaggccgtcc ccccgtttga cgagatactc
      661 ctcgacagcg gccatcgctc gcgtcgcgcg ccccttgtcc gcagcgcccg agatgactcc
      721 ccacgactgg gcgatcgaat cgatccggca ctcctcgctc tgcgcggagc cgaggggcgt
      781 gccgtcgtcg aagtaggccc gccggtacca gtcgccgtcc caggcgttct gctcgagcgc
      841 ggccttgagc gtcacgacgc gctcgcgcca cctctgcgcg cgcgccgtct ccccccgtct
      901 ctcggcgatt cgcgcgaact cccacaggtt cgagtggagg aaccacccga gccacacgct
      961 ctccccttcg ccgcggatgc ccacccggtt catcccgtcg ttccagtccc ccgaccccat
     1021 gagcggcagg ccgtgcttgc ccgccgccaa tcggatgtcg agagcccggg cgcagtgctc
     1081 gaacagggtc gccgactccc gcgacaccgc gggctggaag tagttttcga cctggcccgg
     1141 ctcgagctcc ggcccctcga gaaacgggac gacctcgtcg agaacggtct cgtcgcccgt
     1201 gacctcgacg tagtggatcg tcgcgtaggg gagccagagg aggtcgtcgg agattcgcgt
     1261 gcggaccccg cggcccgagg gggaatgcca ccagtgctgc acgtcccctt caacgaactg
     1321 ccgggacgcc gcgacgagca ggtgctcacg cgtgaccttg cgcgccgcaa ccgtgagcgc
     1381 catgacgtcc tggagctggt cgcggaaccc gtacgcgccg ccggactggt agaaggcggt
     1441 ccgcccctag agccggcacg acagcgcctg atagagcagc cagcgattga cgaacacgtc
     1501 gagcgccggg tcgggggtct tgatctgcag accgccgagg acgccgtccc agaaacgccg
     1561 gatctgacgg agcgtggcat ccgcgtcctt ctcccgatgc gccgtcacca ggcgccgcgc
     1621 ctccgcctcg tcccgcgcct cccccaggaa gacccgcacc tcggcgcgct ctcccggcgc
     1681 gagcgtgat
//
LOCUS       DASWAV010000147         4965 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U2665
            k141_208709, whole genome shotgun sequence.
ACCESSION   DASWAV010000147 DASWAV010000000
VERSION     DASWAV010000147.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960316
            Sequence Read Archive: SRR10260007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 4965)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4965)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:47:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,929
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4965
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_208709"
                     /isolate="SMAG_U2665"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.36444 N 132.31889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(81..740)
                     /locus_tag="VF958_02880"
     CDS             complement(81..740)
                     /locus_tag="VF958_02880"
                     /inference="COORDINATES: protein motif:HMM:NF016176.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HEX9148083.1"
                     /translation="MKSRIRSVAAGLAVLLAIAASAAAAPETFVFDKAHTKVGFQIRH
                     WLTKVDGRFRDFDGKIVIDRGDPAISKVDVTIQAASIDTSNERRDADLKSANFFDVEK
                     FPTITFRSSKVEPKGKDLYEVTGDMTMHGVTKTVKVPVRHTGFLNLGRVEKAGFEGAF
                     PIDRKDFGITWNRTADQGGVMLGDDVDITLLIEANRELTPPVATPAAPAGEATKAPSH
                     P"
     gene            863..3724
                     /locus_tag="VF958_02885"
     CDS             863..3724
                     /locus_tag="VF958_02885"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011311403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003994 - aconitate hydratase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aconitate hydratase"
                     /protein_id="HEX9148084.1"
                     /translation="MSSRPDSAFGAFDSFSVGPAATGNLYSLPKLEERGIGPVSRLPV
                     SIRIVLESLLRNVDGRRVRERDVVELANWKPNADRTAEIPFVVARIILQDFTGVPLLV
                     DLAAMRSAVDRVGRAPSLIEPLVPVALVVDHSIQVDYWASPDALRLNMEVEFKRNRAR
                     YEFLKWGTQAFRGFEVVPPGVGIVHQVNMERLASGVIEKDGVYFPDTLVGTDSHTTMI
                     NSMGIVGWGVGGIEAEAGMLGQPVYILTPDVVGVHMTGRLAENVTATDLALHVTEMLR
                     AAKVVGKFVEFHGEGAASLPVTDRAPIANMAPEYGATMGFFPIDEETCRYLLATGRDP
                     GRVQTYRSYYEAQGLFGMPGSGEIAYTQLLELDLGVVQPSVAGPRRPQDRIPLPALGR
                     TFRNLLVKPPADGGYGKPADAITRRFPTRIGVDGTVPEKPLVGGGKQESGPVLSGHDT
                     NPVTELEMVTNRPAPTRVEEVPEEHLPPAQVDLGHGDVLIAAITSCTNTSNPSLMIGA
                     ALLAKKAVERGLSVPPYVKASFAPGSRVVTDYLRRAGLQTYLDRLGFHLVGYGCTTCI
                     GNSGPLDPRVEEIVTSHDLITASVLSGNRNFEARIQQSIKANFLMSPPLVVAFALAGS
                     IDRDLSKDPIGGNGGGKVYLRDIWPTSEEVRAAAKSSVDPETYHRLYGGGAEENPLWN
                     DIPAPTGEVYSWDPESTYIQEPPFFENFSMTPAAVTDVKAARALGIFGDFVTTDHISP
                     AGAIKKTSPAGLYLQERGVPPEDFNSYGSRRGNDRVMTRGTFANVRIRNLMVPGEEGG
                     VTIYQPHGDRLSIFEAATRYQAAGVPLLVFAGQEYGTGSSRDWAAKGTRLLGVRAVIT
                     QSYERIHRSNLVGMGVLPCQFPEGVSVRTLGLDGTESFDVTGVAEGVRPRGEVKLTIR
                     RSDGRVDEVPLRVRIDTPIESDYYRHGGILQYVLRNLIA"
     gene            3766..4824
                     /gene="hemE"
                     /locus_tag="VF958_02890"
     CDS             3766..4824
                     /gene="hemE"
                     /locus_tag="VF958_02890"
                     /EC_number="4.1.1.37"
                     /inference="COORDINATES: protein motif:HMM:NF013381.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase"
                     /protein_id="HEX9148085.1"
                     /translation="MTTTSPSAKRFLDACFGRPVDRAPAWMMRQAGRYLPEYREVRHR
                     VSFIDLCRDADLAAEVSLQPFRRFSPDGVVFFSDILVPVQAMGARVEFGHEGPRLSHP
                     VRTGDDVLRLRRFDPSGELRFTGEILKKLRAELLDSAAVIGFAGAPWTLACYLVEGGG
                     SKSFAAIKQMMGWGQRTLEGLLNLLADVVGDVLSYQIASGAQVVQIFDTWAGELSQED
                     YRRWALPAVTRAIKGIRRDGQPVILYVNGCAHLIEAMNESGADVLSIDWRLPLSEARR
                     KLPTRPLQGNLDPGALLAGTRDVRRRVRSMLAETGGIAHVVNLGHGVLPQTPIESVEA
                     FFSAVRESGVAEPAEAQA"
     gene            4821..>4965
                     /locus_tag="VF958_02895"
     CDS             4821..>4965
                     /locus_tag="VF958_02895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011804527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="coproporphyrinogen III oxidase"
                     /protein_id="HEX9148086.1"
                     /translation="MNEKRLSVADVSVDLLRRYNVPGPRYTSYPTAPVWREGFGPREY
                     ENVL"
BASE COUNT          801 a         1685 c         1618 g          861 t
ORIGIN      
        1 gcatcgagga cgagatggta acccgtccga actgaccggc ccccgggtcc cggagtatcg
       61 gagttcggcc ctgatttctc ctaggggtgc gacggcgcct tcgtggcctc tccggcgggc
      121 gctgccggag tcgcgacggg cggcgtcaat tccctgttcg cttcgatgag gagtgtgatg
      181 tcgacgtcgt cgccgagcat cacccctccc tggtcagcgg ttcggttcca ggtgatcccg
      241 aagtccttgc ggtcgatcgg gaacgccccc tcgaagcctg ctttttcgac acgacccaga
      301 ttcagaaagc cggtatgtcg gaccgggacc ttcacggtct tcgtgacgcc gtgcatcgtc
      361 atgtcgcccg tgacctcgta gaggtccttt cccttcggct cgaccttcga gcttcggaac
      421 gtgatcgtcg gaaacttctc gacgtcgaag aaattcgccg acttcaggtc cgcgtcccgg
      481 cgctcgttgc tggtgtcgat ggacgccgcc tgaatcgtca cgtcgacttt cgagatcgcc
      541 gggtctcctc gatcgatcac gatcttgccg tcgaaatccc ggaaacgacc gtccaccttc
      601 gtcaaccagt ggcggatctg gaaaccgact ttcgtgtgag ccttgtcgaa aacgaaagtt
      661 tcgggcgcgg cggctgccga cgcggcgatc gcgagcagca ctgcgaggcc ggcggcgacg
      721 ctgcgaattc gagacttcat ctctgttctc tccttgttcc ggggccaatt acgcccggaa
      781 gggccgacgg gttgcgcgcc ttccttgacg ctttcgatcg cccgaccata gactgcgaac
      841 tgtaaatggt cgattccttc cgatgagctc gcggccggat tccgccttcg gcgcattcga
      901 cagcttttcc gtcggcccgg ccgccaccgg gaacctctac tcgctgccga agttggagga
      961 gcggggaatc gggccggtgt cccgcctgcc ggtcagcatc cggatcgttc tcgagtctct
     1021 cctccgcaac gtcgacggac gccgggtgcg ggaacgggat gtggtggagc tcgccaactg
     1081 gaaaccgaac gccgaccgca ccgccgaaat tccgttcgtc gtcgcgcgca tcattctcca
     1141 ggacttcacc ggagttccgc tcctcgtcga cctcgctgcg atgcgatccg cggtcgatcg
     1201 cgtgggtcgg gcgccctccc tgatcgaacc gctcgtgccc gttgcgctcg tcgtcgatca
     1261 ctcgatccag gtggactact gggcgagtcc cgacgcgctg cggctgaaca tggaggtcga
     1321 gttcaagcgc aaccgcgcgc gatacgaatt cctgaagtgg ggcacgcagg cgttccgagg
     1381 cttcgaagtc gttcccccgg gcgtcgggat cgtgcaccag gtgaacatgg agcggctcgc
     1441 gtccggggtg atcgagaaag acggcgtcta cttccccgac acgctggttg gcaccgactc
     1501 tcacacgacg atgatcaaca gcatggggat cgtcggatgg ggcgtcgggg gcatcgaggc
     1561 ggaagcgggc atgctcgggc agcccgttta catcctcacg cccgacgtcg tgggcgtcca
     1621 catgaccggc cgcctcgccg agaacgtcac ggcaaccgac ctcgcgctcc acgtcacgga
     1681 gatgctgcgg gccgcgaagg tcgtcgggaa attcgtcgag tttcacggcg aaggcgcggc
     1741 gtcgcttccg gtgaccgacc gcgcgccgat cgcgaacatg gcgcccgagt acggcgccac
     1801 gatggggttc ttcccgatcg acgaagagac ctgccggtat cttctcgcga ccgggcgcga
     1861 tcccgggcgg gtgcagacgt accgcagcta ctacgaggcg cagggcctct tcggcatgcc
     1921 cgggagcggc gagatcgcct acacgcagct tctcgagctc gacctcggcg tcgtgcaacc
     1981 gagcgtcgcg ggcccgcgcc ggccgcagga ccggatcccc ctgcccgcgc tcggccggac
     2041 gttccgcaac ctgctcgtga agccgccggc ggacggcggc tacggaaagc ccgccgacgc
     2101 gatcacgcga cggtttccga cccggatcgg agtcgacggg accgtgcccg agaagccgct
     2161 cgttggcggt ggcaagcagg agtcgggtcc cgtcctctcc ggccacgaca cgaacccagt
     2221 aaccgagctc gagatggtca ccaaccgccc ggcgccgacg cgggtcgagg aggtgccgga
     2281 agagcacctt cccccggctc aggtcgacct cggccacggc gacgtgctga tcgcggcaat
     2341 cacgtcctgc acgaacacct cgaacccgag cctcatgatc ggtgctgcgt tgctcgcgaa
     2401 gaaggcggtc gagcgggggc tgtccgtgcc accgtacgtg aaagcgtcgt tcgcgcccgg
     2461 atcacgcgtc gtgaccgact acctgaggag ggccggcctc cagacctacc tcgaccggct
     2521 cgggttccat ctcgtcggat atggctgcac gacgtgcatc gggaactccg gccccctcga
     2581 tccgcgcgtc gaggaaattg tcacgtccca cgacctcatc accgcatcgg tgctctccgg
     2641 caatcgaaac ttcgaagctc ggatccagca gagcatcaag gcgaacttcc tcatgagtcc
     2701 cccgctcgtc gtggcgttcg ccctggcggg gagtatcgac cgcgacctgt cgaaagaccc
     2761 catcggcggc aacggcggcg ggaaggtcta tctgcgcgac atctggccga cgtccgagga
     2821 ggttcgcgcc gcagcgaagt cctccgtgga cccggagacc taccatcgcc tctacggcgg
     2881 cggcgcggag gagaacccgc tctggaacga cattcccgcg ccaacgggcg aggtctattc
     2941 gtgggacccc gagtcgacgt acatccagga gccgccgttc ttcgagaatt tctcgatgac
     3001 gccggccgcg gtcaccgacg tcaaggcggc gcgcgcgctc gggatcttcg gggacttcgt
     3061 gacgaccgac cacatcagcc cggcgggggc catcaagaag acgagtcccg caggcttgta
     3121 tcttcaggag cggggagtcc cgccggagga cttcaacagc tacggctcgc ggcggggcaa
     3181 cgatcgcgtc atgacgcggg gcacgttcgc aaacgtccgg atccgaaacc tcatggttcc
     3241 cggagaggaa ggcggggtca cgatttacca gccgcacggc gatcgcctgt cgatcttcga
     3301 agcggcgacc cggtaccagg ccgccggggt tccgctcctc gtgttcgcgg gacaggagta
     3361 cgggaccggc agctcgcgcg actgggcggc caaggggacg cgactactcg gcgtccgcgc
     3421 cgtcatcacc cagagctacg agaggatcca ccgctcgaat ctcgtcggca tgggcgttct
     3481 cccctgccag ttcccggaag gcgtgagcgt gcgcacgctc ggcctcgacg gaacggaatc
     3541 cttcgacgtg accggggtcg cggagggcgt gcggccgcgg ggcgaggtca agctcacgat
     3601 ccggcggtcg gatggccgcg tcgacgaggt gccgctgcgg gtccgcatcg atacgccgat
     3661 cgagtcggac tactaccgac acggcgggat ccttcagtac gtcttgagga acctgatcgc
     3721 ctgagtcttt tccaacgctc gttatactga cgggccgtac acgaaatgac gaccacctcc
     3781 ccttcggcga aacggttcct cgacgcctgt ttcgggcgcc ccgtggaccg cgcgcccgca
     3841 tggatgatgc ggcaggccgg acgttatctg cccgaatacc gcgaggttcg ccaccgcgtt
     3901 tctttcatcg acctgtgccg ggacgccgac ctcgccgcgg aagtttcgct gcagccgttt
     3961 cggcgattct cgccggacgg ggtcgtgttc ttctccgaca ttctcgtgcc cgtacaggcg
     4021 atgggcgccc gtgtcgaatt cgggcacgaa gggccccgac tttcccaccc cgtgcgcacc
     4081 ggtgacgatg tcctgcgcct gcgccgcttc gatccttccg gcgagctccg gttcaccggg
     4141 gaaatcctca agaagctccg cgcggagctc ctcgacagtg ccgcggtcat cggcttcgcc
     4201 ggtgcgccgt ggacgctcgc gtgctacctg gtcgaggggg gcgggtcgaa gtccttcgcc
     4261 gcgatcaagc agatgatggg ctggggccag cgcacgctcg agggacttct caacctcctc
     4321 gccgacgtcg tcggggacgt gctctcctac cagatcgcct ccggcgcgca ggtcgtccag
     4381 atcttcgaca catgggcggg agagctttcg caggaggact accggcgttg ggcgctgccg
     4441 gcggtcaccc gggcgatcaa gggaatccgg cgcgacgggc agcccgtcat tctctacgtg
     4501 aacggctgcg cgcacctgat cgaggcgatg aacgagtcgg gcgccgacgt cctctcgatc
     4561 gactggcgcc tgccgctgtc cgaagcgcgc cggaagcttc cgacgcgccc cctccagggc
     4621 aacctggatc cgggcgccct tctcgccggc acgcgtgacg ttcggcgccg cgtgcggtcg
     4681 atgctcgccg agaccggcgg catcgctcac gtggtcaacc tcggccacgg agtcctgccg
     4741 cagactccga tcgaatccgt ggaggccttc ttctccgccg ttcgcgagtc gggcgttgcc
     4801 gagcccgccg aggcgcaggc gtgaacgaga aacgtctctc cgtcgcagac gtttcggtgg
     4861 atctgctccg caggtacaac gtcccgggcc cccgctacac ctcgtacccg accgctcccg
     4921 tatggagaga gggcttcgga ccgcgggagt acgagaacgt cctct
//
LOCUS       DASWAV010000148         4941 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U2665
            k141_327367, whole genome shotgun sequence.
ACCESSION   DASWAV010000148 DASWAV010000000
VERSION     DASWAV010000148.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960316
            Sequence Read Archive: SRR10260007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 4941)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4941)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:47:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,929
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4941
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_327367"
                     /isolate="SMAG_U2665"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.36444 N 132.31889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..461)
                     /locus_tag="VF958_02900"
     CDS             complement(<1..461)
                     /locus_tag="VF958_02900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018716340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly protein"
                     /protein_id="HEX9148087.1"
                     /translation="MRMEKETTTTREVTASEIPAGTRFTIPAGTRLFITQSLGGSFTV
                     MMPMGYLARVDGKDADAIGEEPPEQPDEAAAAGKSTQDLSWDQLRTCYDPEIPVNIVD
                     LGLVYACEVAPLPDGGSRVDVKFTLTAPGCGMGDVLREDIRQKLLTVPGVR"
     gene            complement(461..955)
                     /locus_tag="VF958_02905"
     CDS             complement(461..955)
                     /locus_tag="VF958_02905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009770556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SUF system NifU family Fe-S cluster assembly
                     protein"
                     /protein_id="HEX9148088.1"
                     /translation="MGDLRDLYQEVILDHSKRPRNFGELAGANRHAEGYNPLCGDRET
                     VYVRLENGVLEDVGFRGAGCAISTASASMMTESVKGKTLAEADALFERFHDLITGNDG
                     ATKTSPPDLGKLAVFSGVREFPVRVKCATLPWHTLKAALAGEDKTVSTELTEGPKAES
                     GGAS"
     gene            complement(955..2223)
                     /locus_tag="VF958_02910"
     CDS             complement(955..2223)
                     /locus_tag="VF958_02910"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020249435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0006534 - cysteine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HEX9148089.1"
                     /translation="MSISASVHEKPSQSRGDRLDVAALRRDFPILARKIHGKPLVYLD
                     NAATTQKPAVVIDAVADMYRTYYGNIHRGVHLLSVEVTDAYEAVREKARAFVNAAEAR
                     EIVFVRGTTEGINLVSQTFGRQRVGEGDEVVITALEHHSNIVPWQLLCEEKKARLKVI
                     PIDRSGAVRVEELERLITPRTRIVSIAHVSNALGTLVPVRRIVEIAHARGVPVLIDGA
                     QAAPRLALDVRAIGCDFYTFSSHKMYGSTGVGVLYGRADLLDEMPPYQGGGDMISSVT
                     FEKTTWNVLPYKFEAGTPNIAGAIGLGAAIDYLSAIGMDNVTAHEEDLLAYATRSLTA
                     IPGLNVIGTAPEKIGVLSFTLDGIHPHDVGTVLDQEGIAIRTGHHCAQPVMEFFGIPA
                     TARASLALYNTREEIDVLAEGIRKVQRMFR"
     gene            complement(2220..3524)
                     /gene="sufD"
                     /locus_tag="VF958_02915"
     CDS             complement(2220..3524)
                     /gene="sufD"
                     /locus_tag="VF958_02915"
                     /inference="COORDINATES: protein motif:HMM:TIGR01981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HEX9148090.1"
                     /translation="MSAVAEETAKAPFERSLEESFRESRSPRDPQWLVEIRRAAMERL
                     REIGFPTVRDEDWKYTNVAPILKVPFAAPPEGGPRRVEPTSEGAQLVFVNGRYASELS
                     SAAASGVQIGSLRDVLAKQPELAEPYLGRIARNGNAFASANAAFIDDGAFVRIPLGLA
                     AAEPIHLLFLSEPAFDPTVSHPRNLVIAEAGSQAAVVETYIGTAGETYFTNPVTEVFV
                     GDGANLDLYKLERESTAAYHVATTEVRLERDCRFASHSLTFGGAIARNDLNVRLEAEG
                     ADCTLNGLFVGSGTQHLDNHTLIDHAKPHGTSRELYKGIMDGKSRGVFHGKIIVRPDA
                     QKTDAMQTNKNLLLSKEALVNSIPALEIFADDVKCRHGSTIGQLDPGAMFYLQSRGIG
                     AEEARQLLVYAFASDVASRIRIAPLRALVEEHLGLRLAGGHA"
     gene            complement(3524..4303)
                     /gene="sufC"
                     /locus_tag="VF958_02920"
     CDS             complement(3524..4303)
                     /gene="sufC"
                     /locus_tag="VF958_02920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014702905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HEX9148091.1"
                     /translation="MALLEIQNLHASVGGNEILRGVDLTVDSGEVHAIMGPNGSGKST
                     LAHVLAGRPGYQVTEGRVIYKGQDLLAMTPEIRAREGVFLAFQYPVEIPGVSNVYFLK
                     NAVNAVRKHRGLPELDAFDFLALVKEKAKLVEIDEELVKRPVNEGFSGGEKKRNEIFQ
                     MAILAPTLAILDETDSGLDIDALRIVASGVNSLRSPERAMIVVTHYQRLLNYIVPDFL
                     HVLVEGRIVREGGKELAYELEEKGYAWLEKEPPAPAAAPIP"
     gene            complement(4305..>4941)
                     /locus_tag="VF958_02925"
     CDS             complement(4305..>4941)
                     /locus_tag="VF958_02925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006162013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="SufD family Fe-S cluster assembly protein"
                     /protein_id="HEX9148092.1"
                     /translation="NWYPGDEQGRGGIFNFVTKRGKCLGRKSKISWTQVETGSAITWK
                     YPGCILQGDGSVGEFYSVAVTNNYQQADTGTKMIHIDKDTKSTIVSKGISAGHGQNSY
                     RGLVKILGKADNARNYSQCDSLLIGDKCGAHTFPVIEVLNSTAQMEHEASTSKIGEDQ
                     VFYFQQRGISPEEAVNMIVSGFCKVVFKELPMEFAVEAQKLLGVSLEGSVG"
BASE COUNT          760 a         1617 c         1633 g          931 t
ORIGIN      
        1 tcgcgcactc cgggcaccgt gagcagcttc tggcggatgt cctcgcgcag aacgtccccc
       61 attccgcaac cgggcgccgt caacgtgaat ttgacgtcga ccctgctgcc gccgtccggc
      121 agtggcgcga cctcgcaggc atagacgagc ccgaggtcga cgatgttgac cgggatttcc
      181 gggtcgtagc acgtgcggag ctggtcccac gagaggtcct gggtggattt cccggcggcc
      241 gcggcctcgt ccggctgttc cggaggctcc tcgccgatcg cgtcggcgtc cttgccgtcg
      301 acacgggcga ggtatcccat cggcatcatt accgtgaagg acccgcccag ggactgagtg
      361 atgaagagtc gcgttcccgc cgggatcgtg aacctcgttc ccgccgggat ctcgctcgcg
      421 gtgacttcgc gcgtcgtcgt ggtctctttc tccattcgca tcagctcgct ccgccgctct
      481 cggccttcgg accttcggtg agctcggtcg agaccgtctt gtcttctccc gcgagcgcgg
      541 ccttcaacgt gtgccagggc agcgtcgcgc acttgacccg caccgggaac tcgcgcactc
      601 ccgagaagac ggcgagcttt ccgaggtcgg gcggggacgt cttcgtcgcg ccgtcgttcc
      661 cggtgatgag gtcgtggaag cgctcgaaga gcgcgtcggc ctcagcgagc gtctttccct
      721 tgacgctttc ggtcatcatc gaggcggacg cggtcgagat cgcgcagccc gcgccgcgaa
      781 agcccacgtc ctccagcacg ccgttctcga gccgaacgta gacggtctcg cggtccccgc
      841 agagcgggtt gtacccctct gcgtggcggt tggctcccgc gagctcgccg aagttccgcg
      901 gccgcttgct gtggtcgagg atgacttcct ggtagaggtc gcgaaggtcg cccatcagcg
      961 gaacatcctc tggactttgc ggatcccttc cgcgagcacg tcgatctctt ctcgagtgtt
     1021 gtagagcgcg agcgatgcgc gcgcggtcgc cggaattccg aagaactcca tcaccggctg
     1081 cgcacagtgg tggcccgtgc ggatcgcgat gccttcctga tcgagcacgg tgccgacgtc
     1141 gtgcgggtga attccgtcga gagtgaagga gagcacgccg atcttctcgg gcgcggtgcc
     1201 gatgacgttc agacccggaa tcgccgtcag gctccgcgtc gcatacgcga gcaggtcctc
     1261 ctcgtgcgcg gtgacgttgt ccatgccgat cgccgagaga tagtcgatcg ccgcgccgag
     1321 cccgatcgcg cccgcgatgt tcggtgtgcc cgcctcgaac ttgtagggga gcacgttcca
     1381 cgtcgtcttc tcgaacgtga cggagctgat catgtctccg cccccctggt agggcggcat
     1441 ctcgtcgaga aggtccgcgc gcccgtacag caccccgacg ccggtggatc cgtacatctt
     1501 gtgcgaggag aacgtgtaga agtcgcagcc gatcgcgcgc acgtcgagcg cgagtcgcgg
     1561 cgccgcctgt gcgccgtcga tcagcaccgg gactccgcgc gcatgagcga tctcgacgat
     1621 ccgtcggacc gggacgagcg tgccgagagc gttcgagacg tgcgcgatcg agacgatccg
     1681 ggtgcggggc gtgatgaggc gctcgagctc ctcgacccgc accgcgccgg agcggtcgat
     1741 cgggatcacc ttgagacgcg ccttcttttc ctcgcagagg agctgccagg ggacgatgtt
     1801 cgagtggtgc tcgagcgcgg tgatgacgac ctcgtcgcct tcgccgaccc gctgccggcc
     1861 gaaagtctgc gagacgaggt tgatgccctc ggtcgtgccc cgaacgaaga cgatctcgcg
     1921 cgcctcggcg gcgttcacga aagcgcgcgc cttctcgcgt accgcttcgt acgcgtccgt
     1981 cacctcgacc gagagcaggt gcacgccccg gtggatgttg ccgtagtacg tccggtacat
     2041 gtccgcgacc gcgtcgatga cgacggcagg cttctgagtc gtcgccgcgt tgtcgaggta
     2101 gacgagaggc ttcccgtgga tcttccgcgc gagaatcggg aaatccctcc ggagggcggc
     2161 aacgtcgagg cgatcgccgc ggctctggct cggcttttcg tgaaccgaag cgctgatgct
     2221 catgcgtggc ctccagctag ccgcaacccg aggtgctctt cgaccagggc gcggagcggc
     2281 gcgatccgga tgcgagaggc gacgtcgctc gcgaaggcat acacgaggag ttgacgggcc
     2341 tcctccgcgc cgatgccgcg cgattgcagg tagaacatcg ccccgggatc gagctgcccg
     2401 atcgtcgatc cgtggcggca cttcacgtca tccgcaaaga tctccagggc ggggatcgag
     2461 ttgacgagcg cctctttcga gaggagcagg ttcttgttcg tctgcatcgc gtcggtcttc
     2521 tgcgcgtccg gacgcacgat gatctttccg tggaagactc cgcgggactt tccgtccatg
     2581 atccccttgt agagctcgcg gctcgtgccg tgcggcttcg cgtggtcgat caaggtgtgg
     2641 ttgtccaggt gctgcgttcc ggagccgacg aagagtccgt tcagcgtgca gtcggcgcct
     2701 tccgcctcga ggcggacgtt caggtcgttg cgggcgatcg cacccccgaa agtcagcgaa
     2761 tgggaagcga accggcagtc gcgctcgaga cgcacttccg tcgtcgcgac gtggtacgcc
     2821 gccgtgctct cgcgctcgag cttgtagagg tcgaggttcg ctccgtcgcc gacgaagacc
     2881 tccgtgaccg ggttggtgaa gtaggtctcg ccggcggtgc cgatgtacgt ctcgacgacg
     2941 gccgcctgac tgccggcctc cgcgatcacg aggttgcgcg ggtgcgacac ggtcggatcg
     3001 aaggccggct ccgacaggaa aaggagatgg atgggctccg cggccgcgag tcccagcggg
     3061 atccgcacga acgcgccgtc gtcgatgaac gcggcgttgg ccgaggcgaa cgcgtttccg
     3121 ttgcgcgcaa tccgaccgag gtatggctcg gcgagctccg gctgcttcgc caggacgtct
     3181 ctcaggctcc cgatctggac tccgctcgcg gcagccgagg agagctcgga ggcgtaacgc
     3241 ccgttgacga aaacgagctg agctccctca ctcgtcggct cgacgcgacg cggtcccccc
     3301 tccggtggcg cggcgaacgg gaccttgagg atcggcgcga cgttcgtgta cttccagtct
     3361 tcgtcgcgaa cggtggggaa tccgatttcg cgcagccgct ccatcgcggc gcgccggatc
     3421 tcgactaacc actgcggatc gcgcggcgag cgcgattcac gaaaggactc ctccagggag
     3481 cgctcgaacg gggccttcgc ggtctcctcg gcgacggcgg acatcacggg atcggagcgg
     3541 cggccggcgc gggcggttcc ttctcgagcc aggcgtagcc cttctcctcg agctcgtagg
     3601 cgagctcctt gcctccctcg cgaacgatgc gcccttcgac gagcacgtgc aggaagtcgg
     3661 gaacgatgta gttcaagagg cgctggtagt gcgtgacgac gatcatcgcc cgctcgggac
     3721 tccgaaggga gttcaccccg gacgccacga tccgcagcgc gtcgatatcg agacccgagt
     3781 cggtctcatc gagaatggcg agcgtcggag cgaggatcgc catctggaag atctcgttcc
     3841 tcttcttctc gccgcccgag aatccctcgt tgaccggccg tttgacgagc tcctcgtcga
     3901 tctccacgag ctttgccttc tccttgacga gcgcgaggaa atcgaacgcg tcgagctccg
     3961 gcaagccgcg gtgctttcgg accgcattga ccgcgttctt gaggaagtag acgttcgaga
     4021 cgcccgggat ctcgaccggg tactgaaacg cgaggaatac gccctcgcgc gcgcgaatct
     4081 ccggcgtcat cgcgagcagg tcctgcccct tgtagatgac ccggccttcc gtgacctggt
     4141 agccgggccg cccggcgagc acgtgcgcga gcgtgctctt tcccgatccg ttcggtccca
     4201 tgatcgcgtg gacttcgccc gagtcgacgg tcaggtcgac gccgcggagg atctcgtttc
     4261 cgccgacgct cgcgtggagg ttctggatct cgagaagcgc catctcagcc gaccgatcct
     4321 tccagtgaca cgccgaggag cttctgtgcc tcgaccgcga actccatcgg aagctccttg
     4381 aagacgactt tgcagaagcc ggacacgatc atgttgaccg cctcttccgg cgagatcccg
     4441 cgctgctgga agtagaagac ctggtcttcg ccgatcttcg atgtcgatgc ctcgtgctcc
     4501 atctgggctg tcgagttcag cacctcgatc accgggaagg tgtgtgcgcc gcacttgtcg
     4561 ccgatcaaga gcgagtcgca ctgcgaatag ttgcgggcgt tgtcggcctt tccgagaatc
     4621 ttgacgaggc cgcgatagct gttctggccg tgccccgcgg agattccctt cgaaacgatg
     4681 gtcgatttcg tgtccttgtc gatgtggatc atcttggtgc ccgtgtcggc ctgctggtag
     4741 ttgttggtca cggcgaccga gtagaactct ccgaccgagc cgtcgccctg caggatgcag
     4801 cccgggtact tccaggtgat cgcggagccc gtctcgacct gcgtccacga gattttcgac
     4861 ttgcgcccga ggcacttccc gcgcttcgtg acgaagttga agatgccgcc gcggccctgc
     4921 tcgtcgcccg gataccagtt c
//
LOCUS       DASWAV010000149        12790 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U2665
            k141_648051, whole genome shotgun sequence.
ACCESSION   DASWAV010000149 DASWAV010000000
VERSION     DASWAV010000149.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960316
            Sequence Read Archive: SRR10260007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 12790)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12790)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:47:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,929
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12790
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_648051"
                     /isolate="SMAG_U2665"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.36444 N 132.31889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..803
                     /locus_tag="VF958_02930"
     CDS             <1..803
                     /locus_tag="VF958_02930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="exo-alpha-sialidase"
                     /protein_id="HEX9148093.1"
                     /translation="HDDGTPTEHGFVSAVAEGDRIRIFWLDGRETAASPAGHDGHSGE
                     GAMTLRTALVGDRVETSDLLDRRVCDCCQTAAAMTANGPVVAYRDRSDHEIRDVALVR
                     RENGRWTTPRPVHDEGWEIAGCPVNGPAVAADGRHVAVAWYTQGANRPRVQVAFSQDA
                     GASFAPPVVVDRNGPLGRVDVVLDENGDALVLWVASEGKAAAIRLARVTPAGKVGTAL
                     AVSATETSRSSGFPRLERAGSNLVLAWVGPSEPFRVRAATLPVAAVSP"
     gene            complement(800..2827)
                     /locus_tag="VF958_02935"
     CDS             complement(800..2827)
                     /locus_tag="VF958_02935"
                     /inference="COORDINATES: protein motif:HMM:NF023418.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminaseTgpA domain-containing protein"
                     /protein_id="HEX9148094.1"
                     /translation="MTTLPMSSDLRRAYLLVLGPPALIAPLPLFWTHGASAIAIALYE
                     TALILLFFRARVGRPVRLSDAVLNTIGLTYLFWLGVETALLRPGLIRSVSHLLLFTAI
                     GKLASLKRPSEARTALLVIFLVVLASVSSSTHVISLLYCAVMAVLAFRTLARLAVLAD
                     FEEGPPLRVLKSVPTPGLSAAAILAGALLTAPLFFVLPRLHGPYALSPIRLEDAASTA
                     VATDRVDLDAFGGAKRSDRVVLRLSVDPPVAYPELLRLREAVFTQYRRGVWTRSARAA
                     REVRRDTRLRFVTDDSLPPEARLARLSADENLFGSGFLFLPYRSTHVRVERGRPVLLP
                     DGTVQVSSSRSTVRYEVSVGKEEARAEGEVAIDPGSVPAEVADYARKLTGAITDPPQI
                     AAAIIDHFRRGFIYTLDPPKGLGDPVVNFLLRSKAGHCEYFASAAAMMLASRGVPSRL
                     VTGSYGGEVGALSELIVVRGTNLHAWVEAELDGTGFQMLDPTPPAGLPPATARVAWWK
                     RLLTVGREIEFFYDRRILGFDSGEQIQLTESFRESFGNATRWLASWKGAPGGVLPGGA
                     KIVVVLFALSVLALMALRGLARRPVVPPATRAYLALRRLLGQRVGAAVGPGVAPLEVA
                     RRFEAAAPASGGDARAVVNAYCEDAFGGRPTDPATARELRERLRRLKKLAG"
     gene            complement(2824..3810)
                     /locus_tag="VF958_02940"
     CDS             complement(2824..3810)
                     /locus_tag="VF958_02940"
                     /inference="COORDINATES: protein motif:HMM:NF013998.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HEX9148095.1"
                     /translation="MTRLIPDWGVRVTNFGLGYILMCLVVAIAATNTGNNGLYLVLAG
                     LLAGMAVSGMLSRRNVRGVRCEIEPLGEIVATRPSLLKVRLVNELRAGTAQALFFLHE
                     ALPGPLWIDPLAAGESREIVVEAVFRRRGVFREADAGLLSRFPLGLFRKYRRATVAGE
                     IVVFPVPRESAVPDVPPEDARGRRPHPHRRGGGAEIRTLRDFVPGDDPRDLHWKQSAR
                     MRRWIVREREAERDRAVFLVVDNALADPGDPAALERLEQAISHCAGQALMLLSRGAEV
                     GFAARGVKVPPRAGRRQRAPILDTLARLEPVPSNEAPAFPPMRKGDHRQVIT"
     gene            complement(3818..4759)
                     /locus_tag="VF958_02945"
     CDS             complement(3818..4759)
                     /locus_tag="VF958_02945"
                     /inference="COORDINATES: protein motif:HMM:NF040105.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HEX9148096.1"
                     /translation="MTVAIASELARLRENIGRAYRGERRTIDLVLTALLSRGHILIED
                     VPGVGKTTLARALGRSLSLDFRRIQFTSDTLPADVLGISVFHAASDRFEFHPGPLFAN
                     IVLADEINRATPKTQSSLLEAMNEGAVTVESERRELPQPFMVVATQNPVEYLGTYPLP
                     ESQMDRFFIRLSLGYPGPEAEKELLRTGGTDRELATLPAVLERSQVFELQKAAESVRV
                     ADRLLDYLHGLVSATRKAGDLRLPVSTRGAQALYRATQSYALVSGRDYATPDDAQAVA
                     EPVLAHRILSVSSDGFGAGAREKEFVKRVLAQVAVPV"
     gene            complement(4756..5745)
                     /locus_tag="VF958_02950"
     CDS             complement(4756..5745)
                     /locus_tag="VF958_02950"
                     /inference="COORDINATES: protein motif:HMM:NF012695.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-1-phosphate guanylyltransferase"
                     /protein_id="HEX9148097.1"
                     /translation="MRILLLAGGGGTRLWPLSTSARPKQFLPLVSQRSLLADTRERVA
                     PVADAIFVATSEEHVGLVRGELPDVPVDHILPEPARRNSAAPIVRAALDFESDGDPVT
                     AAVPSDQAVSDPGAFRRALTAAARLADAAPVVVLAVRPDRPGTDFGYLEVADREGSNG
                     YAVSRFTEKPDLGEARSFLASGRHFWNAGIFVFRPSRFLVEARRVAPDLVTGVEHYRN
                     LLSGDPAGARAAWESLPAISIDYAVMERARGVRAVELSAGWSDVGTWKSVREMRGASD
                     GSGNLILSDRPVLAPGVRDTAIVVGDEGVLVLPFERESDLRGAVEGLRRGTRQ"
     gene            complement(5757..6668)
                     /gene="murB"
                     /locus_tag="VF958_02955"
     CDS             complement(5757..6668)
                     /gene="murB"
                     /locus_tag="VF958_02955"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: protein motif:HMM:NF010480.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008762 - UDP-N-acetylmuramate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HEX9148098.1"
                     /translation="MRVGPVTLELAERDGTVLEDEPLSRHTTLKIGGPARRYCRVRTE
                     EGLSLVVNAALRSGEPLALLGMGSNILAADEGYAGWVIRLEGDFLALRVDGDRLVAGG
                     GAALGGACAAAARAGLSGLESISGIPSSIGGAVRINAGAYGGEIFDVLETVRLVGRDG
                     ERRLARAADIPHGYRWTKLCDTGEIVAEATLRLFQASREAIDERTRVVSEKRRGALPG
                     EPNAGSIFKNPSGDYAGRLIEACGLKGERRGGAAISTRHANVIVNTGGARAADVLALM
                     RLMRESVREKFGVTLAAEVEFLGLTWE"
     gene            complement(6650..7222)
                     /locus_tag="VF958_02960"
     CDS             complement(6650..7222)
                     /locus_tag="VF958_02960"
                     /inference="COORDINATES: protein motif:HMM:NF016489.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HEX9148099.1"
                     /translation="MKTISPISAGPASRAKLASALRERPLATILAIGFGVGLAPIAPG
                     TAGSALALAAGWIAVAVFSSHMPSVVAGVGLLTSGLLVGAAAVPVTGQVATLLGAEDP
                     GCIVLDEIAGQLIASAVVPLFAYSSAETAAGLWLASFFAFRLFDVWKPGPIRGWQNLP
                     GGWGIVVDDVAAGVCAAGFTAALAACVSGL"
     gene            7348..8430
                     /gene="recA"
                     /locus_tag="VF958_02965"
     CDS             7348..8430
                     /gene="recA"
                     /locus_tag="VF958_02965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018353605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008094 - ATP-dependent activity, acting
                     on DNA [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HEX9148100.1"
                     /translation="MSSVKPNEKWRAVETALTQIERQFGKGSIMRLGDRPEVPVAVIS
                     TGSIAVDAALGIGGIPRGRVVEIFGPESSGKTTLSLQIIAQAQKTGGLAGFIDAEHAL
                     DADYAKKLGVDTDNLLVSQPDNGEQALEIAEMLIRSGAIDVIVVDSVAALVPKAEIEG
                     EMGEAQMGLQARLMSQALRKLTAIVSKSRTSLIFINQIREKIGVMFGNPETTTGGRAL
                     KFYSSIRIDIRRVASLKDGDVVVGSRTKVKIVKNKVASPFRQAEFDINYGEGISRTGE
                     LIDLGLEAKIVEKSGAWLSYGELRIGQGRENAKQFLKENPELAAEIEGKVRKSLGLAP
                     VETKPAPAAAPSVVAETPREKEKVRR"
     gene            8483..11287
                     /locus_tag="VF958_02970"
     CDS             8483..11287
                     /locus_tag="VF958_02970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9148101.1"
                     /translation="MKKRGRFGNARAIAGVFLFLAGIVSSARADRLTVAAERPTGPCP
                     AGGALGIVQVPSGRDLPPSVERCLVLFEVGDLSDAGVLSDAGRLAKTTGATAVVLDFT
                     RFSQTPDAASRARLPFAIKQLSSAVRGASPGARVGVDFSRGPGEPFSLDLEEGLGVYF
                     DAIATFAGRQPPSFAEEGKERWLFLLRTPGRAAASEIVQAVVSAEMVPQLVGIFSTPE
                     ATITDSDRESLSRLQRYWTADVSHDPTATTATRVDGTRFPVLRFFDGKKFTPILLLAE
                     DPAGRATIELSGGPYAKASVENLSSGAKRDFELNGAKTFELDLSRGPLAVVLEPAKRP
                     DERTTVQVGAARGLTAEEIIARERAWEAGQHENVSSFIANMEASLRFRVAEVNETFDL
                     TIRGPYFLRRGEAGDWVWQDFYLNGVKWKGKTLPKIPILQPEKVTTLPLDIRLTEDYR
                     YELAGTSEVGGQRAYEINFTPKETLGDRPVYRGKVWIDARTFALLRRDSVQLNLKGDT
                     LSNVQSELHSPVPGRTDVFLPLEIKGQQVFSTAGRTTAIEGLVKLTNVEIDPADFEAR
                     RKAAYASDLQMIRDTEHGMRYLVPDPARPGERVVEEKVSKKSTFGLAGGFYDDSLSSP
                     IPLLGIQHFNFDLWGKGKQLSVFFGGALLTANYTDPSLGGGRFDLGADLFAVAFPFGD
                     VAYRDGKEVPGEKIKHLPAIFQINLGRPLGPYVKTSLGIFTRWDNFQRDPDTASNFIT
                     PVDTFTDGAELRLVGNYKGFNATAVGGYYRRREWTLWGDPDTTEFDQRHKDYLKYQLA
                     LSKDQYFPGFRKLHVAMTYLDGQDLDRFSKYEFGAFSGAPIHGFKSGSVKTQTALVGT
                     VSYGLNIEDIIRFEAIFDQAVVRDRRSGYDNTYFAGAGLLGSLNGPWQNSLLRFEIGV
                     PVVSHGVKGFVVNVILLKLF"
     gene            complement(11259..11501)
                     /locus_tag="VF958_02975"
     CDS             complement(11259..11501)
                     /locus_tag="VF958_02975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008328982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA family protein"
                     /protein_id="HEX9148102.1"
                     /translation="METDLEVDARGLLCPLPILRLARALRRAPGGTVALLLATDPAAV
                     EDVNVLCREEGHELLSTEREGAVLRFRVRKASRGSR"
     gene            11526..>12790
                     /gene="purH"
                     /locus_tag="VF958_02980"
     CDS             11526..>12790
                     /gene="purH"
                     /locus_tag="VF958_02980"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: protein motif:HMM:NF002049.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0003937 - IMP cyclohydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0004643 -
                     phosphoribosylaminoimidazolecarboxamide formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HEX9148103.1"
                     /translation="MPEPIPFPKSVAPVRRALISVHDKSGVAQLALGLAAAGVRVVST
                     GGTAAHLKQAGVEVSLVEEETGFPEILGGRVKTLHPKIFGGVLADATRADHARDMEAA
                     AIEAFDLVVVNLYPFEETVARGAARADTIEMIDIGGTALIRAAAKNHARVAVVVEPTD
                     YDDVLEEIRETGGTRLETREKLAARAFARTAGYDAAIARYLAEGRRPEEGFPENLVFV
                     FDRIGSLRYGENPHQRAALYRDRAAPADALVRFEMLQGKELSFNNLLDLDSAVTLARD
                     FDRPAAVIVKHNNPCGAAVGDTVLEAFGRAFACDPLAAFGGIIAIRGRVDGALASLVL
                     QHFVEAVAADEFTQEALDFFTKKPNVRLLRLPVSRRPAATLDWKRIGGGLLLEDDDSE
                     PDQPSAWRIASQRKPTAVERAACEVAWKVV"
BASE COUNT         2157 a         4382 c         4436 g         1815 t
ORIGIN      
        1 cgcacgacga cgggacgccg accgagcacg gtttcgtctc cgcggtcgcg gaaggcgatc
       61 ggatccggat cttctggctc gacggccgcg agaccgcggc ctcccccgcc ggtcacgacg
      121 ggcattccgg ggaaggcgcg atgacgcttc gcaccgcgct cgtcggagac cgggtcgaaa
      181 cgagcgacct cctggaccgc cgggtctgcg actgctgcca gaccgcggcc gcgatgacgg
      241 cgaacggccc ggtcgtcgcg taccgcgacc gatccgacca cgaaatccgc gacgtcgccc
      301 tcgtgcgccg ggaaaacggc cgctggacga ccccccgacc cgttcacgac gaaggctggg
      361 aaatcgcggg atgtccggtc aacgggccgg ccgtcgccgc cgacgggcgc cacgtcgcgg
      421 tcgcgtggta cacgcagggt gcgaaccggc cccgggtcca ggtcgcgttt tcgcaggatg
      481 cgggcgcctc gttcgctccc cccgtcgtgg tggaccgcaa cgggccgctc ggtcgggtcg
      541 acgtcgttct cgacgaaaac ggcgacgctc tcgtcctgtg ggtcgcgtcc gaaggaaagg
      601 ccgcggcgat ccggctggcc cgcgtgactc ccgctgggaa ggtcggcacc gccctggcgg
      661 tctcggcgac ggagacgagc cgctcgagcg gctttccgcg cctcgagcgg gcgggaagca
      721 acctcgtgct cgcctgggtc gggccgagcg agcccttccg cgttcgcgcg gcgacgttgc
      781 ccgttgcggc ggtgtcgccc tagcccgcga gcttcttcaa cctccgcagg cgctcccgaa
      841 gctcgcgcgc ggtcgcggga tccgtcggcc ggccgccgaa ggcgtcctcg cagtaagcgt
      901 tcaccacggc gcgcgcatcg ccgcccgacg ccggcgccgc cgcctcgaaa cgccgcgcga
      961 cttcgagagg cgcgacgccc ggcccgaccg cggcgccgac gcgctgcccg agaaggcgcc
     1021 ggagcgcaag ataggcgcgt gtcgcgggtg ggacgaccgg ccggcgcgcg aggccgcgca
     1081 gcgccatcag ggcgaggacg gagagcgcga acaggacgac cacgatcttc gcgccgccgg
     1141 gaaggacgcc gcccggcgct cctttccagg acgcaagcca ccgggtcgcg ttgccgaacg
     1201 actcgcggaa gctctcggtc agctggatct gctcccccga gtcgaagccg aggattcggc
     1261 ggtcgtagaa gaattcgatt tcacgaccca ccgtcaggag gcgtttccac caggcgacgc
     1321 gcgcggtcgc cggcggcagt cccgcgggcg gcgttggatc gagcatctgg aaaccggtgc
     1381 cgtccagctc cgcctcgacc cacgcgtgga ggttggtccc ccggacgacg atcagttccg
     1441 acagggctcc gacctctccg ccgtacgagc ccgtgacgag tcgcgatgga acaccgcgcg
     1501 aggcgagcat catcgcggcc gccgacgcga agtactcgca atggccggct ttggaacgca
     1561 gaaggaagtt gacgaccgga tcgccgagcc ccttcggcgg atcgagcgtg taaatgaagc
     1621 ctctccggaa gtggtcgatg atcgcggccg caatctgcgg cgggtcggtt atcgcaccgg
     1681 tcaactttcg ggcgtagtcg gcgacttccg cgggaacgct tccgggatcg atcgcgacct
     1741 ctccctcggc gcgcgcctcc tccttcccga cggagacctc gtatcgaacg gtcgatcggc
     1801 tcgaggaaac ctggacggta ccgtcgggca agagcaccgg gcggccgcgc tcgacccgga
     1861 cgtgagtgct gcggtacggc aggaagagga agccgctgcc gaacagattc tcgtcggccg
     1921 aaagccgggc gagccgagcc tcgggtggga gcgagtcgtc ggtgacgaag cgaagcctcg
     1981 tgtcgcggcg aacctcgcgc gccgcgcgcg cgctgcgggt ccagacaccg cggcgatact
     2041 gcgtgaagac cgcctcgcga agccggagga gctccggata cgcgaccggg gggtcgaccg
     2101 agagccggag aactacccga tcgctgcgct tcgcacctcc aaaggcatcc agatccacgc
     2161 gatcggtcgc caccgcggtc gaagcagcgt cctcgagccg gatcggcgac agggcgtacg
     2221 gcccgtgcag ccgcggcagc acgaagaaga gaggggcggt caggagcgcg cccgcgagga
     2281 tcgccgcggc ggagagaccg ggcgtcggca cggatttcaa cacgcgaagc ggcggcccct
     2341 cttcgaagtc cgcgagcacc gcgagccggg cgagagtgcg aaacgcaagg accgccatga
     2401 ccgcgcagta gaggagcgag atgacgtggg tcgaagagga gaccgacgcg agcacgacga
     2461 ggaagatcac gagcagcgcc gtccgagcct ccgacggccg cttcagggac gcgagcttcc
     2521 cgatcgcggt gaagaggagg aggtgcgaga cggagcggat gaggccgggg cgcaggagag
     2581 ccgtttcgac gccgagccag aaaagatagg tgaggccgat ggtattgagc acggcgtcgg
     2641 agagtcgcac cggccggccg acgcgcgcgc ggaagaagag caggatgagc gcggtttcgt
     2701 agagcgcgat cgcgatggcg gatgcgccgt gcgtccagaa cagcgggagc ggagcgatca
     2761 acgccggagg cccgagaacc agaaggtacg cgcgccgcag atccgaggac atcggaaggg
     2821 tcgtcacgtg atcacctgcc ggtggtctcc cttacgcatc ggcggaaatg cgggcgcctc
     2881 gttcgaaggg accggttcga ggcgcgcgag cgtatccagg atcggagcgc gctgccgcct
     2941 ccccgctcgc ggcgggacct tgacaccccg cgccgcgaat ccgacctcgg ctccgcgcga
     3001 caggagcatc agcgcctgcc cggcgcagtg ggagattgcc tgttccaacc gttcgagcgc
     3061 cgccggatcg ccaggatcgg cgagggcatt gtcgacgacg agaaaaacgg cgcggtcgcg
     3121 ctccgcctcg cgctctcgaa cgatccaccg ccgcatccgc gccgactgtt tccagtgaag
     3181 gtccctcggg tcgtctcccg ggacgaagtc gcgcagcgtc cggatttccg ccccgccgcc
     3241 gcgacggtgc gggtgaggac ggcgcccgcg agcgtcctcg ggcggcacgt cgggaaccgc
     3301 gctttcccgg ggaaccggga agacgacgat ctcgcccgcg acggtcgccc ggcggtactt
     3361 gcggaagagc ccgaggggaa agcgcgaaag gagcccggcg tccgcttccc ggaagacgcc
     3421 tcgccggcga aacaccgctt cgacgacgat ctcgcgcgac tcgcccgccg cgagcggatc
     3481 gatccagaga gggccgggca gcgcctcgtg caggaaaaag agcgcctgcg cggtccccgc
     3541 tcgaagctcg ttgacgaggc gcaccttcag gagcgacgga cgcgtcgcga cgatctcgcc
     3601 gagaggctcg atctcgcacc gcacgcctcg gacgttgcgg cgggagagca tcccggaaac
     3661 agccatgccc gccaggaggc cggcgagaac gaggtacagc ccgttgttgc ccgtgttggt
     3721 ggccgcgatc gcgacgacga ggcacatcag gatgtacccg aggccgaagt tggtgacccg
     3781 gacgccccag tcggggatga ggcgagtcat cacacgccta aacgggcacc gccacctgcg
     3841 cgagaactct cttgacgaat tccttctcgc gcgcgccggc cccgaagccg tccgacgaga
     3901 ccgagaggat ccggtgggcg agcactggct cggcaaccgc ctgggcgtcg tccggggtcg
     3961 cgtagtcgcg gccggagacg agcgcgtacg actgcgtcgc ccgatagagc gcctgcgcgc
     4021 cacgggtgga aacggggagg cgcaggtcgc ccgccttgcg cgtcgcggac accagtccgt
     4081 gcaggtagtc gagcaagcgg tcggcgacgc gcacgctctc ggcggccttc tgcagctcga
     4141 acacctggga gcgctcgagc acggcaggga gagtcgcgag ctcgcgatcc gtgcctccgg
     4201 tccgcaggag ctccttctcc gcctcgggtc cgggatagcc cagggagaga cggatgaaga
     4261 agcggtccat ttgcgactcc ggaagagggt aggtgccgag atactcgacg ggattctgag
     4321 ttgccacgac catgaacggc tgcggcagct cgcgccgctc cgactcgacg gtcaccgcgc
     4381 cctcgttcat cgcctcgagg agagaagact gcgtcttcgg agtcgctcgg ttgatctcgt
     4441 cggcgagcac gatgttggcg aagagcggcc cggggtggaa ctcgaaccgg tcactcgccg
     4501 cgtgaaacac ggagatcccg aggacgtccg ccggaagcgt gtcgctcgta aactggatcc
     4561 gccggaagtc gagcgacagg ctgcggccga gagcgcgggc gagcgtcgtc tttcccaccc
     4621 cgggcacatc ctcgatcagg atgtggccgc gcgagaggag ggcggtcaaa acgaggtcga
     4681 tcgtgcgcct ctcgccgcgg taggcgcgac cgatgttctc ccggagccgg gcgagttcgg
     4741 aggcgatcgc gaccgtcact gccgggtccc ccgccgcagc ccctcgactg cgccccgaag
     4801 gtcgctctcc ctctcgaacg ggaggacgag cactccctcg tctcccacca cgatcgctgt
     4861 gtcccgcacg ccgggagcga gtacgggccg gtccgagagg atcaggtttc cggagccgtc
     4921 ggaggcgccc cgcatctcgc ggacggattt ccatgttccg acgtcactcc agccggccga
     4981 gagctcgacg gctcggaccc cccgggcgcg ctccatgacg gcgtagtcga tcgagatcgc
     5041 cggcaaggac tcccaggcgg cgcgggcgcc ggccgggtcg ccgctcagaa ggtttcggta
     5101 gtgctcgacc cccgtcacga ggtccggcgc cacgcggcgg gcctcgacga ggaagcgcga
     5161 gggccgaaag acgaagatcc cggcgttcca gaagtgccgc ccggaggcaa gaaacgaccg
     5221 ggcctctccg aggtccggct tctcggtgaa ccttgagacc gcgtaaccgt tggagccctc
     5281 cctgtccgcg acttcaagat aaccgaaatc ggtgccgggc cggtccgggc gcacggcgag
     5341 caccaccacg ggggccgcgt cggccagccg cgccgccgcg gtcagcgctc ggcgaaacgc
     5401 gccgggatcg gagactgcct gatcggaggg cacggccgcg gtgaccgggt cgccgtcgct
     5461 ctcgaagtcg agcgccgccc ggacgatcgg tgccgcggaa ttccggcgtg ctggctcggg
     5521 gaggatgtgg tcgaccggca cgtcgggcag ctctccccgg acgagaccga cgtgctcctc
     5581 cgacgtcgcg acgaagatcg cgtcggcgac gggagcgacg cgctcccggg tatcggcgag
     5641 caggctccgt tgcgagacga gcggcaggaa ttgcttcggg cgagcagagg tcgagagcgg
     5701 ccacagacgc gtgccgccgc ccccggcgag gagcaggatg cgcattcccg attgtctcac
     5761 tcccaggtca ggccgaggaa ctcgacttcg gcagcgaggg tgacgccgaa cttctcgcga
     5821 accgactcgc gcatgaggcg catgagggcc aggacgtcgg cggcgcgggc gccccccgtg
     5881 ttgacgatga cgttcgcgtg gcgggtcgag atggccgcgc cgccgcggcg ctcccctttc
     5941 agaccgcacg cttcgatgag acgccccgcg taatcgccgg aggggttctt gaagatcgaa
     6001 ccggcgttgg gttcccccgg cagggcgccg cggcgcttct cgctgaccac gcgggtcctc
     6061 tcgtcgatcg cttcgcggga cgcctggaag agccgcaggg tcgcttcggc gacgatctcc
     6121 cccgtgtcgc agagcttcgt ccaacggtac ccgtgaggga tgtccgcggc gcgggcgagg
     6181 cgcctctccc cgtcgcggcc caccagccga accgtctcca gtacgtcgaa gatttcgccg
     6241 ccgtaggcgc ccgcgttgat gcggacggcc cctccgatcg aggaagggat gcccgaaatc
     6301 gactcgaggc ccgagagtcc tgcacgggcc gccgccgcgc aggcgccgcc gagcgcggcg
     6361 ccgcctccgg ccacgaggcg atccccgtcc acccgcaggg cgaggaagtc cccttcgagc
     6421 cgaatgaccc agccggcgta cccctcgtcc gcggcgagaa tgttcgatcc catcccgagc
     6481 agcgccagcg gctcgccgga ccggagggcg gcgttcacga cgagcgagag gccttcctcc
     6541 gttcggaccc ggcagtatcg gcgcgccggc ccgccgatct tgagcgtcgt gtgccgcgag
     6601 agcggctcgt cctcgaggac cgttccgtct cgctcggcga gctccagggt cacaggcccg
     6661 acacgcacgc ggcgagcgcc gccgtgaagc cggccgcgca cacgccggcc gcgacgtcgt
     6721 caacgacgat cccccagccg ccggggaggt tctgccatcc ccggatgggg cccggcttcc
     6781 acacgtcgaa gagccggaac gcgaagaaag acgccaacca gagcccggcg gcggtctccg
     6841 ccgaggagta cgcgaagagc ggaacgacgg ccgacgcgat gagctgcccg gcgatctcgt
     6901 cgagcacgat gcagcccggg tcttccgccc ccaggagggt cgcgacctgt ccggtcaccg
     6961 gcaccgccgc ggcacccacg aggagcccgg acgtcaacag tccgaccccc gcgacaacac
     7021 tgggcatgtg agaagaaaaa accgcgacgg cgatccagcc ggccgcgagc gcgagagcgg
     7081 agccggccgt gcccggcgcg atgggcgcga gcccgacgcc gaacccgatc gcgaggatcg
     7141 tcgcgagcgg tcgttcgcgc agggcgctcg cgagcttcgc ccgcgaggcg ggcccggcgg
     7201 agatcggtga gatcgttttc atcggattcg aagcgcgctt cgacggaagg tccacggggc
     7261 gtcgaccacg gaacggtccg tgcttttccg gagtctataa tcgcgcaaat tccgcggaga
     7321 cgggagcccg atcaggaagg tgaggacatg tccagcgtga aaccgaacga gaagtggcga
     7381 gccgtcgaga cggcgctcac gcagatcgag cgacagttcg gcaagggctc gatcatgcgc
     7441 ctgggagacc ggccggaagt gccggtcgcg gtgatctcga ccggatcgat cgcagtggac
     7501 gccgctctcg gcatcggcgg tatcccgcgc ggccgggtcg tcgagatatt cgggccggag
     7561 tcgtcgggca agacgaccct gtcactgcaa atcatcgccc aggcgcagaa gaccgggggc
     7621 ctcgcgggct tcatcgacgc ggagcacgcg ctcgacgccg attacgcgaa gaagctcggc
     7681 gtcgatacgg acaacctcct cgtctcccag ccggacaacg gcgagcaggc gctcgagatc
     7741 gcggaaatgc tcatccggtc gggggcgatc gatgtgatcg tcgtcgactc cgtggcggcc
     7801 ctcgtcccca aggcggagat cgagggcgag atgggcgaag cgcagatggg cctgcaggcg
     7861 cgcctcatgt cgcaggcgct gcgcaagctg accgcgatcg tgtcgaaatc gcgtacgagc
     7921 ctgatcttca tcaaccagat ccgcgaaaag atcggcgtga tgttcggaaa cccggaaacg
     7981 acgaccggag gccgggcgct caagttctat tcctcgatcc ggatcgacat ccgtcgagtc
     8041 gcgtcgttga aggacggcga cgtcgtcgtc ggctcgcgca cgaaggtgaa gatcgtcaag
     8101 aacaaggtcg cgtctccgtt ccgccaggcg gagttcgaca tcaactacgg cgaggggatc
     8161 tcgcgaaccg gcgagctgat cgacctcggc ctcgaggcga agatcgtcga gaagtcgggg
     8221 gcgtggctct cgtacggaga gctgcggatc gggcaggggc gcgagaacgc gaaacagttc
     8281 ttgaaggaga accccgagct cgccgcggag atcgaaggga aggtgcgcaa gtcgctcggc
     8341 ctcgcgcccg tggagacgaa acccgcgccc gccgcagccc cttcggtcgt ggccgaaact
     8401 ccgcgtgaga aggagaaagt ccgccggtag cgacggctga cgcgggaaca actcccgcgc
     8461 tcgtgccgtt ctgcggttct cgatgaagaa acgagggcga ttcggaaacg cccgcgcaat
     8521 tgcgggcgtt tttctttttc tcgccgggat tgtcagctcc gccagagccg atcgcctgac
     8581 ggtggccgcg gagcgcccga ccgggccgtg tcccgccggc ggcgctctcg gcatcgtgca
     8641 ggtcccgtcc ggccgagacc taccgccgtc cgtcgaacga tgcctcgtcc tcttcgaggt
     8701 cggcgatctc tccgatgccg gcgttctctc cgacgccggc cggctcgcga agacgacggg
     8761 cgcgacggcc gtcgttctcg acttcacccg gttttcccag acgcccgacg ccgcgtcgcg
     8821 cgctcggctc cctttcgcga tcaagcaact ctcgtcggcg gtccggggcg cctccccggg
     8881 cgcccgcgtc ggagtggatt tctcgcgcgg gcccggcgag ccgttctccc tcgacctcga
     8941 ggaggggctc ggcgtgtatt tcgacgcgat cgcgactttc gccggccgcc aaccgccgtc
     9001 cttcgcggag gagggcaaag agcgctggct cttcctcctg cgcactcccg ggcgggcggc
     9061 cgcgagcgag atcgtgcagg ccgtcgtatc ggcggagatg gtgccccagc tcgtcggcat
     9121 cttctcgacc cccgaggcga ccatcaccga ctcggaccgc gagtcgctct cccggctcca
     9181 acgctactgg acggccgacg tctcgcacga tccaaccgcg acgaccgcga cgcgcgtgga
     9241 cggaacgcgg tttccggtgc tgcgcttctt cgacgggaag aagttcacac cgatcctcct
     9301 cctcgccgag gatccggccg gccgcgccac gatcgagctc agcggcggcc cgtacgcgaa
     9361 agcatccgtg gaaaatctct cctcgggtgc gaagcgcgat ttcgagctga acggcgcgaa
     9421 gacgttcgag ctcgatctct cgcgcggccc gctcgccgtc gtgctcgaac cggcaaagcg
     9481 ccccgacgag cgcacgacgg tccaggtggg agcggcgcgc ggcctgaccg ccgaagagat
     9541 catcgcgcga gaacgggcgt gggaggcggg tcagcacgag aacgtgtcct cgttcatcgc
     9601 gaacatggag gcatcgctgc gcttccgcgt cgccgaggtg aacgaaacgt tcgacctcac
     9661 gatccggggt ccctactttc tccgtcgcgg cgaggcggga gactgggtct ggcaggactt
     9721 ctacctgaac ggcgtcaagt ggaagggcaa gacgctcccc aagatcccca tcctgcagcc
     9781 ggaaaaagtg acgacccttc ctctcgacat ccgcctgacg gaggactacc ggtacgagct
     9841 cgccggcacc tccgaggtcg gcgggcagcg ggcttacgag atcaacttca cgccgaagga
     9901 gaccctcggc gaccggcccg tttaccgcgg caaggtctgg atcgacgccc ggacgttcgc
     9961 gctgctgcgt cgcgactccg ttcagctcaa cctgaagggg gacaccctct cgaacgttca
    10021 gtccgagctc cacagccccg ttcccggaag gaccgacgtc tttctgcccc tcgaaatcaa
    10081 gggccagcag gtgttttcca cggccggccg gacgaccgcc atcgagggac tcgtgaagct
    10141 caccaacgtc gaaatcgacc cggcggattt cgaggcgcgg aggaaagccg cgtacgcgtc
    10201 ggacctccag atgatccgcg acaccgagca cggcatgcgg tacctcgtgc ccgatcccgc
    10261 gcgccccggc gaacgtgtcg tcgaggaaaa ggtctcgaag aagagtacgt tcgggctcgc
    10321 cggcggcttc tacgacgact ccctcagctc ccccataccg cttctcggta tccagcactt
    10381 caacttcgac ctctggggga agggcaagca gctctccgtc ttcttcggcg gcgcgctcct
    10441 gacggccaac tacaccgacc catcgctcgg cggcgggcgg ttcgatctgg gcgccgacct
    10501 cttcgccgtt gccttcccgt tcggagacgt tgcctaccgc gacggcaagg aggtcccggg
    10561 cgagaagatc aaacacctgc cggccatatt ccagatcaac ctcggccggc cgctcgggcc
    10621 ctacgtgaag acgtcgctcg gcatcttcac ccgctgggac aacttccagc gcgacccgga
    10681 taccgcttcc aacttcatca cgccggtcga caccttcacc gatggcgcgg agcttcggct
    10741 ggtcggaaac tacaagggct tcaacgccac ggcggtcggc ggctattacc ggcggcgcga
    10801 gtggacactc tggggcgacc cggacaccac ggagttcgac cagaggcaca aggactacct
    10861 caagtaccag ctcgcgcttt cgaaagacca gtactttccg ggattccgga aactgcacgt
    10921 cgcgatgacg tacctcgacg ggcaggatct cgaccgattt tcgaagtacg agttcggcgc
    10981 gttctccggc gcgccgatcc acgggttcaa gagcggaagc gtcaagacgc agacggcgct
    11041 cgtggggacg gtgtcgtacg gcttgaacat cgaagacatc atccgattcg aggcgatttt
    11101 cgatcaggcc gtcgtgcgcg accgccggtc gggatacgac aacacctact tcgcgggggc
    11161 gggcctcctc ggttcgttga acggtccctg gcagaactcc ctgctccggt tcgagatcgg
    11221 cgtgcccgtc gtctcccacg gggtcaaggg cttcgtcgtc aacgtgatcc tcttgaagct
    11281 tttctgacgc ggaatcgcag gacggctccc tcgcgctccg tcgacagcag ctcgtgaccc
    11341 tcctcccggc acaggacgtt gacgtcctcg acggcggccg gatcggtcgc gagaaggagc
    11401 gccacggtgc cgccgggcgc acggcggaga gcgcgggcga gccgcaggat gggcagcggg
    11461 cacaggagac cgcgcgcgtc gacttcgagg tcggtttcca tcgccgctag ctatactcct
    11521 ctgcgatgcc cgagcccatc ccgtttccca agtcggtagc tccggtgcgc cgggcgctca
    11581 tctcggtcca tgacaagtcc ggcgtcgcgc agctcgctct cggcctcgcg gcagccgggg
    11641 tcagagtcgt ctcgacgggc ggcacggccg cgcacttgaa gcaggcgggt gtcgaagtct
    11701 ccctcgtcga ggaggagacg ggatttccgg agatcctcgg cggacgggtc aagaccctcc
    11761 acccgaaaat cttcggcggc gtcctcgccg acgcgacgcg cgccgatcac gctcgcgaca
    11821 tggaggcggc cgcgatcgaa gccttcgacc tggtcgtcgt gaacctctat cccttcgagg
    11881 agaccgtggc ccgcggcgcg gcccgggccg acacgatcga gatgatcgac atcggaggca
    11941 cggcgctgat tcgcgccgcc gcgaagaatc acgcccgggt cgccgtcgtc gtggagccaa
    12001 ctgactacga cgacgtcctc gaagagatcc gggagaccgg cggaacgcgg ctcgagacgc
    12061 gcgagaagct cgcggcgcgc gccttcgccc gaaccgcggg atacgacgcg gcgatcgccc
    12121 ggtacctcgc ggaggggcgt cggccggagg aggggttccc ggagaacctc gtcttcgtgt
    12181 tcgaccggat cggatcgctg cggtacggtg agaacccgca ccagcgggcg gccctctacc
    12241 gagatcgcgc cgcgcccgcg gacgccctcg tgcggttcga gatgctccag ggcaaggagc
    12301 tctctttcaa caatctcctc gacctcgatt cggcggtgac gctcgcgcgt gacttcgatc
    12361 gaccggctgc agtcatcgtc aagcacaaca atccatgcgg agcggccgtc ggggacacgg
    12421 tgctcgaagc gttcggtcgc gcgttcgcct gcgacccgct cgcggctttc ggcgggatca
    12481 tcgccatccg cggccgagtt gacggggcgc tcgcctcgct cgttctgcag cacttcgtgg
    12541 aggccgtcgc cgccgacgag ttcacgcagg aggcgctcga tttcttcacg aagaagccga
    12601 acgtccggct tctccgcctg cccgtctcgc gcaggcccgc ggcgaccctc gactggaagc
    12661 ggatcggagg cggccttctt ctcgaggacg acgactcgga accggaccag ccgtccgcct
    12721 ggcgaatcgc ttcgcagagg aagccgacgg cagtcgagcg cgcggcctgc gaggtcgcct
    12781 ggaaagtggt
//
LOCUS       DASWAV010000150         6302 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U2665
            k141_249000, whole genome shotgun sequence.
ACCESSION   DASWAV010000150 DASWAV010000000
VERSION     DASWAV010000150.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960316
            Sequence Read Archive: SRR10260007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 6302)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6302)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:47:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,929
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6302
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_249000"
                     /isolate="SMAG_U2665"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.36444 N 132.31889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..186
                     /gene="ispH"
                     /locus_tag="VF958_02985"
                     /gene_synonym="lytB"
     CDS             <1..186
                     /gene="ispH"
                     /locus_tag="VF958_02985"
                     /gene_synonym="lytB"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013123270.1"
                     /note="catalyzes the conversion of
                     1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate into
                     isopentenyl diphosphate (IPP) and dimethylallyl
                     diphosphate (DMAPP); functions in the nonmevalonate
                     isoprenoid biosynthesis pathway; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HEX9148104.1"
                     /translation="TSGASAPEFLVEQMVAHLTALGAELVDDLVTVEEDVHFPLPGEL
                     LEARQRRGTVALPTISG"
     gene            277..1053
                     /locus_tag="VF958_02990"
     CDS             277..1053
                     /locus_tag="VF958_02990"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX9148105.1"
                     /translation="MSSRLKGKVALVAGASRGIGEAIVRRFGADGARVALAARDVQAC
                     ERIADEIVRNGGEALALHCDVTDSKSITETVAAVVARWKRIDVLVNNAGVGGSTPLSD
                     PSNALWDAIVATNLTAVFRMTRAAAPHLSDGGRILNMSSVVGRFGVAGLGAYSATKHG
                     VIGLTRSFALELAGRGITVNAICPGWVETDMGRAGWTQMVEGHGGGENEGREVAAKMA
                     PLGRVIEPEEVAGLASYLASNEAASITGQAIVIDGGQVMP"
     gene            1100..1780
                     /locus_tag="VF958_02995"
     CDS             1100..1780
                     /locus_tag="VF958_02995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017305761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CADD family putative folate metabolism protein"
                     /protein_id="HEX9148106.1"
                     /translation="MKSEEFLNALDALVAEKHLLKHPFYTLWSEGKLSRAHIREYAVS
                     YYPHVAAFPTYVSGVHSGCDDPALRIELLDNLVEEEGGSVNHPALWRRFATALGADEE
                     DLRILPRTPEVAAAIAEFRRATREGSVAEGLAALYAYESQIPEISRTKREGLAAFYGI
                     DEPEATRFFTVHEEADVWHRQVEREALGRVAATPVDQERALASAERCLDAMNRALDGV
                     MRENGLAA"
     gene            1791..3785
                     /locus_tag="VF958_03000"
     CDS             1791..3785
                     /locus_tag="VF958_03000"
                     /inference="COORDINATES: protein motif:HMM:NF021126.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhO family protein"
                     /protein_id="HEX9148107.1"
                     /translation="MKTAAAGAALALVVAVAFWPVVTEERSFLQMDLRYEHVPVWHAV
                     QQALRAGESPFWIEGQYCGNPLLFTQEAPPFYPLTLPLLLTGTPANRLSDLFSLIHFW
                     VAGLAAFLLLRDLKCDVASSLFGGVAWMLSARMVQSAIWPNALAVAALLPFVLLGIFR
                     IGSMRRRSGIVVTALSGGICLLAARPHVIVGAVPLLLASCVAAVVFSRRRWRTLADIA
                     LAGVLAAALGAPSVVPSAILMPETSRAAGLDRAERNPYPLATEIDQVFLPVDGQRRWP
                     EAAAYPGVLVLILAGVGVFLALRRGEEFPRAMFSGIVVGAAIGLLFAFGENGPLGFVA
                     NLPLIRGFRIPARYLTSWSLGLALASALALSALLSRTRRPSVIAGVCVLVLATDLVLH
                     ARRSVPDGPSAIYSTEPVLAPLLRARLGKDELGFPRRVWSVVPPPLLWLFDDREKLTL
                     ARSSEPLYGAIGMNFGLEMVGGSGPSPARWKSLFVGVDARRAELAGAGALVLPAPSGF
                     ALSGFPGLPRAIVVPEAVVVRPGRALAAVLDPGLDPRHTAVLEQGSDLEVDPRWTTTA
                     SLRLVSRGPGRLKLAATLPGDGVLVVFNTFEKGWGAKVDGGTQPVVVADYAFQGVRIP
                     AGRHEVELRYRPRGLATGFAIAVVGAFGLLFRARGVVPDP"
     gene            3782..4483
                     /locus_tag="VF958_03005"
     CDS             3782..4483
                     /locus_tag="VF958_03005"
                     /inference="COORDINATES: protein motif:HMM:NF012905.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HEX9148108.1"
                     /translation="MIRAVLLDAGATLIHPDPPVEAVYAREFAADGARFEEDEFRRAL
                     SRAWEEVHSNPEKNRYGGVRGESDFWRAFLNRVRSHLDGGVVSSSAFEKLASHFRSAG
                     AWAVYDDVVATLEELTRLGFTLGIVSNWDSYLPNLLASLGLDRFFPVVAVSAIEETGK
                     PHPEIFRRACRRLDVRPAEALHVGDSVAEDLEGARSAGLSALLLDRQNLHVEIADRIT
                     SLAELPDRLCYPPGA"
     gene            4486..6030
                     /locus_tag="VF958_03010"
     CDS             4486..6030
                     /locus_tag="VF958_03010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007783976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HEX9148109.1"
                     /translation="MVKGLFRTKSLDELVAETEEPGHRLRKVLGPGNLIALGIGAIIG
                     AGIFATIGTATAGDVHRPGAGPALMLSFVLTAVVCGFTALCYAEFASMVPVAGSAYTY
                     SYATLGELIAWIIGWDLILEYAIGNVAVAISWANYFNTLLEGLGIHLPRWLTIDFQTA
                     HSKMPDVVAAAPHVFGIPIVVNVLAFAIVAAITVILVWGIRESASFNFWMVAIKLVVL
                     GFFVVVSFKYVKPANWTPFAPNGFTGIAAGAAIVFFAYIGFDAVSTTAEEARNPKRDM
                     PIGIIGSLIVCTIIYILISAVFTGMIPYPALQQTLATEQAEPLTLAMKYVSMPNWMVG
                     IVAFGSVVAHTAVLLVFQLGQPRIFFAMARDGLLPSTFAKVHPKFRTPHVTTILTGVA
                     VGVTAMFTTLDEMVNLTNIGTLFAFILICLGIVILRFKDPHRERPFRVPFGPVLLPAL
                     GVVSSLGLIYYLPPSSWWRFVGWLAVGSVIYFLYGYRHSRLRHPRVPPGPSDFNPASG
                     LPPSEV"
BASE COUNT          882 a         2174 c         2100 g         1146 t
ORIGIN      
        1 acgtcgggcg cgtcggctcc ggagttcctc gtcgagcaga tggtcgcgca cctgacggcg
       61 ctcggcgcag agctcgtgga cgacctcgtc acggtcgaag aggacgtgca ctttccgctc
      121 cccggggaac tcctcgaggc ccggcagcgg cggggcacag tggcgttgcc gacgatttcc
      181 ggttagccgc gtcgtgactc agtcaccgcc ctcaccccga ccctctcccg gagggagagg
      241 gggcacgggg ccgctcgagg agcgctgtct ctcgaaatga gctcccggct gaaagggaag
      301 gtcgcgctcg tcgccggcgc ttcccgcggg atcggcgaag ccatcgtgcg acgcttcggc
      361 gccgacggag cgcgcgtggc gctcgcagcg cgcgacgtgc aggcctgcga gcggatcgcc
      421 gacgagattg tccggaatgg gggagaggcc ctggcgcttc actgcgacgt gaccgactcg
      481 aagtcgatca ccgagaccgt cgcggccgtc gtggccaggt ggaagcgaat cgacgttctc
      541 gtcaacaacg ccggggtcgg gggttccacg cctctgtccg acccgagcaa tgcgctctgg
      601 gacgcgatcg tcgcgacgaa cctgaccgcg gtcttccgga tgacgcgcgc ggcggcgccg
      661 catctctccg acggcgggcg catcctgaac atgtcgtccg tcgtggggcg gttcggcgtc
      721 gccggactag gcgcgtactc ggcgacgaag cacggggtca tcggcctgac gcgctcgttc
      781 gccctcgagc tcgcgggccg cgggatcacc gtgaacgcaa tctgcccggg ctgggtggag
      841 accgacatgg gccgcgccgg ctggacgcag atggtggagg gacacggggg aggcgagaac
      901 gagggccgtg aagtcgcggc gaagatggcg ccactgggtc gcgtgatcga acccgaggag
      961 gtcgcgggcc tcgcctccta cctcgcgtcg aacgaggccg cctcgatcac cggccaggcc
     1021 atcgtcatcg acggcgggca ggtcatgcca tgattccggg agacggggaa acggaaacgg
     1081 gaaacgaaag gagccgggag tgaaaagcga agagttcctg aacgcgctgg acgcgctcgt
     1141 cgccgagaag cacctcttga agcatccgtt ctacacgctc tggagcgagg gcaagctctc
     1201 gcgggcccac atccgcgagt acgccgtctc ctactacccg cacgtcgccg cgttcccgac
     1261 gtacgtctcg ggcgttcact cggggtgcga cgaccccgcg ctgcggatcg agctgctgga
     1321 caacctcgtc gaggaggagg gcgggtccgt caaccatccg gcgctctggc ggcgcttcgc
     1381 gacggctctc ggcgccgacg aggaagacct tcgaatcttg ccgcgcaccc cggaggtcgc
     1441 ggccgcgatc gccgagttcc ggcgcgccac gcgggaagga tccgtcgcgg aggggctggc
     1501 ggcgctgtac gcctatgaat cgcagatccc cgagatctcg cgcaccaagc gcgaaggtct
     1561 cgccgcgttc tacgggatcg acgagccgga ggcgactcgc ttcttcaccg tccacgagga
     1621 ggccgacgtg tggcatcggc aggtcgagcg ggaagcgctc gggcgcgtgg cggccacgcc
     1681 cgtcgatcag gaaagggctc tcgcatcggc ggaacgctgc ctggacgcga tgaaccgcgc
     1741 gctcgacggc gtgatgcggg agaacggcct ggcggcttag cgatcgatcc gtgaaaaccg
     1801 ccgcggccgg ggccgcgctc gcgctcgtag tcgcggtcgc gttctggccc gtagtgacgg
     1861 aggagcgctc gttcctccag atggacctgc ggtacgagca cgtcccggtc tggcacgccg
     1921 tgcagcaggc cctccgcgcg ggtgagtcgc cgttctggat cgaggggcag tactgcggaa
     1981 acccgctcct cttcacccag gaggcgccgc cgttctaccc gctgacattg cccctcctcc
     2041 tcaccgggac tcccgcgaac cgcctgtcgg atctcttttc actcattcac ttctgggtgg
     2101 cagggctcgc ggctttcctg ctgctgcgcg atctcaagtg cgacgtcgct tcctcgctgt
     2161 tcggcggggt cgcgtggatg ctctcggccc ggatggtcca gagcgccatc tggcccaacg
     2221 ccctggcggt cgcggcgctc ctcccgttcg tgctcctcgg tatcttccgg atcgggtcga
     2281 tgcggcggcg ctccgggatc gtcgtcactg cgctgtccgg gggaatctgc ctgctggctg
     2341 cccgtcccca cgtgatcgtg ggtgcggtgc cgctgctgct tgcctcgtgc gtggctgcgg
     2401 tcgttttttc ccggcggaga tggcggactc tcgccgacat cgcgcttgcc ggtgtgctcg
     2461 ccgccgcact cggagcgccg tccgtggttc cgagcgcgat tctcatgccg gagacctcgc
     2521 gcgccgccgg cctggatcgc gcggaacgca acccctatcc tctcgcgacc gaaatcgatc
     2581 aggtctttct tcccgtcgac ggacagcgtc ggtggccgga agccgcggcg tacccgggcg
     2641 tgctcgtact gatccttgcc ggcgtcggtg tatttctcgc tcttcgccgc ggcgaagaat
     2701 ttccccgcgc gatgttctcg ggcatcgtcg tcggtgccgc gatcggactg ctgttcgcgt
     2761 tcggagagaa tgggcctctc gggttcgttg cgaatttgcc gctcatccgc gggttccgga
     2821 tccccgctcg gtatctgact tcgtggtccc tcggcctcgc cctggcgtcg gcgctcgctc
     2881 tctcggcgtt gctttctcga acacggcgtc cctctgtcat cgcgggcgtc tgtgttctcg
     2941 ttctcgcgac agacctcgtc ctccacgccc ggcgctcggt gccagacggg ccgtctgcga
     3001 tctactcgac cgagccggtt ctcgctccgc tccttcgtgc ccggctcgga aaggacgagc
     3061 tcggttttcc gcgccgagtc tggtcggtcg ttccgccgcc gctcctctgg ctattcgacg
     3121 accgcgagaa gctcacgctc gcccgcagct ccgagccgct ttacggcgcg atcggaatga
     3181 acttcggcct cgagatggtc ggcggttcgg ggccttcacc ggcgagatgg aagtcgctct
     3241 tcgtcggcgt cgacgcccgc agagcggaac ttgccggcgc gggcgcgctg gtgcttcctg
     3301 cgccgagcgg gttcgcgttg tccgggttcc ccggccttcc gcgcgcgatc gtagtgcccg
     3361 aagccgtggt cgttcgaccg ggccgggcac tcgccgcggt gctcgacccg ggtctcgatc
     3421 cccggcacac ggcggtcctc gagcagggga gcgatctcga ggtcgacccg cgctggacga
     3481 ccacggcctc tctgcgtctc gtgtctcgcg gacccggacg cctgaagctc gcggctacgc
     3541 ttcccggcga cggcgttctc gtcgtcttca acaccttcga aaagggatgg ggcgcgaaag
     3601 tcgacggcgg cactcagccc gtcgtcgtcg ccgactatgc cttccaggga gttcgcattc
     3661 ccgccggacg ccacgaggtg gagcttcgat atcgaccgcg cgggctcgcc acaggattcg
     3721 cgattgcggt ggtgggagcc ttcggccttc tgttccgcgc tcgaggggtg gtgcccgacc
     3781 cgtgatccgc gccgtccttc tcgacgcggg cgcgacgctc atccaccccg atcctccggt
     3841 cgaggcggtt tacgcgcgcg aattcgcggc cgacggcgcc cggtttgaag aggacgaatt
     3901 ccgacgcgca ctgtcgcggg cgtgggaaga agttcactcc aaccccgaga agaaccgcta
     3961 cggaggtgtg cgcggcgagt ccgacttctg gcgcgcgttc ctgaaccgtg tccgctcgca
     4021 cctggacggc ggagtcgtct cgtcctccgc attcgaaaag ctcgcgagcc acttccgcag
     4081 cgcgggcgcg tgggcggtat atgacgacgt cgttgccact ctcgaagagc tgacgcgact
     4141 cggcttcacg ctcggcatcg tctcgaactg ggattcgtac ctgccgaacc tcctggcgtc
     4201 gctcggcctc gaccgcttct ttcccgttgt cgccgtctcg gcaatcgagg agacgggcaa
     4261 gccgcatccg gagatttttc gccgcgcctg ccgccgcctg gacgtccgac cggcggaagc
     4321 gctccacgtg ggcgactccg tcgccgagga cctcgagggc gcgcgatcgg ccggactttc
     4381 ggcgctcctg ctcgaccggc agaatcttca cgtcgagatc gcggaccgga tcacgagcct
     4441 cgccgagctc ccggatcggc tctgctatcc tcccggcgct tgagcgtggt caaggggctc
     4501 ttccgcacca agtcactcga cgaactcgtc gcggaaaccg aggagccggg ccaccgcctt
     4561 cgcaaggtgc tcggccccgg caacctgatc gcgctcggca tcggagccat catcggagcg
     4621 ggcatcttcg cgacgatcgg cacggccacg gccggcgacg tgcaccgccc gggagccgga
     4681 ccggcgctga tgctttcgtt cgtgttgacg gcggtcgtct gcgggttcac cgcgctctgc
     4741 tacgcggagt tcgcgtccat ggtgccggtc gccgggtccg cctacacgta ctcctacgcg
     4801 accctgggcg agctgatcgc ctggatcatc ggctgggacc tgatcctcga gtacgcgatc
     4861 ggcaacgtcg ccgtcgcgat ctcctgggcg aactacttca acacgctgct cgaaggcctc
     4921 gggattcacc tgccccgatg gctcacgatc gacttccaga cggcgcactc gaagatgccc
     4981 gacgtcgtcg cggcggcgcc gcacgtcttc gggatcccca tcgtcgtgaa cgtcctcgcg
     5041 ttcgcgatcg tggcggcgat cacggtcatc ctcgtgtggg ggatccggga gtccgcgtcc
     5101 ttcaacttct ggatggtggc gatcaagctc gtcgtgctcg ggtttttcgt cgtggtgagc
     5161 ttcaagtacg tcaaacccgc gaactggacc cctttcgcac ccaacggctt cacgggcatc
     5221 gcggccggcg cggcgatcgt gttcttcgcc tacatcggat tcgacgcggt ctcgacgacg
     5281 gccgaggaag cgcgcaatcc gaagcgcgac atgccgatcg ggatcatcgg ctcgctcatc
     5341 gtttgcacca tcatctacat cctgatctca gcggtgttca ccgggatgat cccgtacccc
     5401 gcgttgcagc agactctcgc gaccgagcag gcggagccgc tgacgctcgc gatgaagtac
     5461 gtctcgatgc ccaactggat ggtcggcatc gtcgcgttcg gatcggtcgt cgcccacacc
     5521 gcggtcctcc tcgtcttcca gctcggtcag ccgcgaatct tcttcgcgat ggcgcgggac
     5581 ggcctcctgc cgtccacgtt cgcgaaggtc catccgaagt tccgcacccc tcacgtcacg
     5641 accatcctga ccggggtcgc ggtcggcgtg acggcgatgt tcacgacgct cgacgagatg
     5701 gtgaacctga cgaacatcgg caccctgttc gcgttcatcc tcatctgtct cgggatcgtc
     5761 atcctcaggt tcaaggatcc gcaccgcgaa cgtccgttcc gcgtcccctt cggcccggtt
     5821 ctccttcccg ctctcggggt tgtctcttcg ctcgggctga tctactacct gccgccgtcg
     5881 tcctggtggc gcttcgtggg ctggctggcg gtcggctcgg tcatctactt cctttacggc
     5941 taccgccaca gccgcctgcg ccatccgcgc gtcccccccg gtccttccga cttcaatccc
     6001 gcgtcggggt tgccaccctc ggaggtttag cagtcctatg ggtcgcggcg cgcgaagtct
     6061 cccgaggtaa tcgaaggagg aggcgcgcgc cgcgggagcg ggagcgagaa gggatcaggc
     6121 ggtcggtcga ggcaggcctg gcgagccgac cgcggtggcg aagccagatc ccgagcgagc
     6181 gagagcgacg ttacggcctg cttcttgcct agccgtccgg catgaatgac gaatcgaacg
     6241 aatcgcgccc ccgcaccgag cttgcgatcc cctggtcgac gatcggaaag atcctcgtcg
     6301 cc
//
LOCUS       DASWAV010000151         5392 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U2665
            k141_408824, whole genome shotgun sequence.
ACCESSION   DASWAV010000151 DASWAV010000000
VERSION     DASWAV010000151.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960316
            Sequence Read Archive: SRR10260007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 5392)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5392)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:47:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,929
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5392
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_408824"
                     /isolate="SMAG_U2665"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.36444 N 132.31889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            257..330
                     /locus_tag="VF958_03015"
     tRNA            257..330
                     /locus_tag="VF958_03015"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:289..291,aa:Gln,seq:ctg)
     gene            424..2955
                     /locus_tag="VF958_03020"
     CDS             424..2955
                     /locus_tag="VF958_03020"
                     /inference="COORDINATES: protein motif:HMM:NF018449.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SBBP repeat-containing protein"
                     /protein_id="HEX9148110.1"
                     /translation="MSLALVLLLAASASGETGRVAEWRVRQRLSGLPISFVANAGQTD
                     PAVAYYAQTFSGTVFVTRDGRIVYSLLQKRDSDSPHGRKASASLPKAANANAGWSLTE
                     TAVGGRASAAAGCLATANVSSFIANDPACWTSSLPTYEDLSLGEVWPGISLQLKARGN
                     SVEKIFTVEPGGDPSKIRMAVRGARSLTVKESGALVLGTGLGEVTFTPPMAYQESGGA
                     RPPVDVAYELRGGDYGFRLGDYDPALPVIIDPLVQSTYLGGGRDDIAVAVAVHPVSGD
                     VYVVGQTTSTNFPGTAGGAQPGAGGGAINAIVARFNPALTALLQATYLGATSDVIANA
                     LAIHPTSGDVYVAGQTSALDFPGTAGGAQPSHAEEAIAGSDDGFVAILNPALTALTGA
                     TYLGGTRDDVVTALAIHPTSGEVYAAGWTLAMNFPGTFGGAQPGSGGGGPSDAFLARL
                     NPALTTLAQATYLGGSSYDLAFGLGIQPISGDVYVAGLTTSTNFPGTAGGAQPTHAID
                     TGLDVFAARLNSTLTALVQATYLGGSGDDSALALAVHPTSGDVFVAGRTYSTNFPATP
                     GGAQPESGGGLTDGFVARLNPALTALAQSTYLGGSFSDFVYALAIPPTSGDVYAAGQT
                     FSANFPFTAAGAQPESGGGEDGFVARLSPALTAPPQATYLGGSGADTATSIAIHPASG
                     DVYVGGWTYSTNFPRMAGGAQPAHALDNGSSDVFVARLTSSLAPWGCSADATTLCLNN
                     GRFKVQTQWTTPQGQSGAGQAVGLTSDTGYFWFFSSNNAEIVAKVVDGRALNSRFWVF
                     AGGLTNVNVVMTVTDTLTGAFKTYTNPQGVAFQPIQDTSAFAALD"
     gene            3048..>5392
                     /locus_tag="VF958_03025"
     CDS             3048..>5392
                     /locus_tag="VF958_03025"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HEX9148111.1"
                     /translation="MTLSAGTRLGSYEILSPLGAGGMGEVYKARDGKLDRDVAIKVLP
                     FSVAGDPDALARFEREAKAVAALSHPNILAIHDFGMHDGTAYAVMELLEGETLGAKLA
                     AGPIPPKQAVDYALQVARGLSAAHEKGIVHRDLKPDNIFVTKDRHVKILDFGLARRVE
                     LFSNDGETSNPTVSSHTQPGTVMGTMGYMSPEQVRGFPADHRSDIFSFGAILYELLTG
                     EKAFKKDTGSDTMAAILRDEPPKPADSGRELSPGLDNIVRHCLEKDRDHRFQSAREIV
                     FALSEGSTLTFPSGVRFVERPPARKRKAISITAAAILLLAVAGVFLWRRSPPSSGEVA
                     AVKRVAVLPFENLGAAEDDYFADGIADEIRSKLTSLPGVEVIARGSSTPYRKTSKTPS
                     QIARELGVGYILTATVRWEKSEGSGRVHVTPEFVEVKKAGAPTAKWQQGFDAALTDVF
                     QVQSAIATRVAQALGVALGAGEEKRLSEKPTQNLAAYNAYLKGEEIWNGTGDDAPSVR
                     KALAFYDQAVALDPAFLQAWARVSWASSILYANSTPTPALAERARSAAEKAIVLAPNR
                     PEGYLAFGHFERLVSKDFNRALEQYAKGQHVAPGNADLLYGTARAEEGLGRWDAAVDR
                     LRQAASVDPRSVVNLTALGYALQRLRRYPEAREFYERGLALAPANLSLIGETAMTFLG
                     EGNLVGARAVLSAVPKEVGSTELVAYIATANDLVWVLDEQQRELLLRLTPSAFDDDRG
                     TWARCLSQASALKGDTANVRAYAEEARQAFEEQLRAAPDNA"
BASE COUNT          909 a         1788 c         1799 g          896 t
ORIGIN      
        1 ctgcggttcg gcgttcgacc gcgaggagaa gacccgcgag cgcgatgaca gtgaccgggg
       61 aaactctctt ccgtgccatg ctggacctcc ttgaccggaa cactctcccc gagcggatcg
      121 gaagaagtcc tctggggaag ccccggaaga cataggtttt cgctaaggag gcaggagaag
      181 gcaaccggct cggacgaaca ggagtgtttg acccgatcga aaggttcgag aataatcgcc
      241 cgttctttcg aacgactggg aggtcgttca atggtaggac aactggctct ggaccagtga
      301 atcggggttc gagtccctgc ctcccagcca agacgtcgcc gaccctgcgc gccgcccctt
      361 gataaggttt ccccggatgc ccgttttcgt ttgatttctg ccagcaggaa ttcgcgcttc
      421 ctattgtctt tggcgctggt gctgctcctt gccgcgagtg cctcgggcga aaccggccga
      481 gtcgccgagt ggcgggttag gcagcggctt tcgggcctgc ccatctcctt cgtcgccaat
      541 gccggccaga ccgatccggc agtggcctac tacgcgcaga ctttctccgg cacggtcttc
      601 gtcacgcggg acggccggat cgtgtattcg ctgctgcaaa agcgagattc ggattcgccg
      661 cacggccgga aggcctcagc ctcgcttccg aaggcagcca acgcgaacgc cggctggtcg
      721 ctgacagaga cagcggtggg cggaagggca tccgcggcgg cgggctgtct ggcgaccgcg
      781 aatgtcagct ccttcatcgc caacgacccg gcgtgctgga cgagcagcct tccgacctat
      841 gaagacttgt cgctcgggga agtctggccg gggatttcgc tccagctcaa ggcgcgcggc
      901 aacagcgtcg agaagatctt tacggtggag ccgggaggag atccctcgaa gatccggatg
      961 gctgtaaggg gcgcgcggtc gctcacggtg aaggagtccg gcgctctggt tctgggaacg
     1021 ggtcttggcg aggtcacgtt cacgccgccg atggcgtatc aggagagtgg aggcgcgcgg
     1081 ccgccggtcg acgtggccta tgagcttcgc ggcggggact acggcttccg actcggcgac
     1141 tacgatccgg ctctgccggt gatcatcgac cccctggtgc agtccacgta tctgggcggc
     1201 ggcagggacg acatcgccgt cgccgtcgcg gtccacccgg tttcgggcga cgtgtacgtc
     1261 gtcgggcaga cgacctccac gaattttccc ggcacggcgg gtggcgcgca accgggcgcc
     1321 ggcggcggcg cgatcaacgc catcgtggcg cgcttcaatc cggctctgac ggcgctcctc
     1381 caggccactt acctgggtgc gacgagcgac gtcatcgcaa acgccctcgc gatccacccg
     1441 acgtcgggcg acgtctacgt ggcggggcag acgagcgccc tcgattttcc gggcaccgcc
     1501 ggcggcgcac agccgtcgca tgcggaggaa gccatcgcgg gaagcgacga cggtttcgtg
     1561 gcgattctca acccggcgtt gacggcgctc accggggcga cgtacctcgg cggcacccgg
     1621 gacgacgttg tgaccgcgct cgcgatccac ccgacgtcgg gtgaggtcta cgccgccggc
     1681 tggactcttg ccatgaactt tcccggcacc ttcggcggcg cgcaaccggg gagcggcggc
     1741 ggcgggccca gcgatgcctt cctcgcgcgt ttgaaccctg ccctgacgac gctcgctcag
     1801 gccacgtacc tgggcggcag cagttacgac ctcgctttcg gcctcgggat ccagccgatc
     1861 tcgggagacg tctacgtggc cgggttgacg acgtcgacga atttccccgg caccgcgggc
     1921 ggcgcccaac ccactcatgc catcgacacc gggctcgatg tgttcgccgc gcgtttgaac
     1981 tcgacgttga cggcgctcgt tcaagccacc tatctgggcg gcagcggcga cgactccgcg
     2041 ctcgcgcttg cggtccaccc gacctcgggc gacgtcttcg tcgccgggcg gacgtactcg
     2101 acgaactttc cagcaacgcc gggtggcgcg caaccggaaa gcggcggggg tctgaccgac
     2161 ggcttcgtgg cgcgcttgaa cccggctctg acggcgctcg ctcagagcac gtacctgggc
     2221 ggcagcttca gcgatttcgt ctacgccctc gcgatccccc cgacgtcggg cgacgtctac
     2281 gccgccgggc agacgttctc cgccaacttt ccgttcacgg cggccggtgc ccaaccggaa
     2341 agcggcgggg gcgaagacgg cttcgtcgcg cgtttgagcc cggcgttgac cgcgccccct
     2401 caggcgacgt acctgggcgg gagcggcgcc gacaccgcca cttcgatcgc gatccacccg
     2461 gcttcgggag acgtttacgt cggcggctgg acgtactcca cgaactttcc gcgcatggcg
     2521 ggcggcgcgc agccggcgca cgctctcgac aacggctcct ccgacgtttt cgtcgcgcgc
     2581 ctgacgtcaa gtctcgcccc ctggggctgc agcgccgacg cgaccacgct ctgcctgaac
     2641 aacggccgct tcaaggtgca gacgcagtgg acgacgccac agggacagag cggcgcgggc
     2701 caggccgtcg ggttgacctc cgataccggc tacttctggt tcttctcgtc caacaatgcc
     2761 gagattgtcg ccaaggtcgt cgatgggcgc gccttgaact cccggttctg ggtcttcgcg
     2821 ggaggcctga ccaacgtcaa cgtcgtgatg acggtgaccg atactctgac aggagcattc
     2881 aagacctaca cgaacccgca aggcgtcgcc ttccagccca tccaggacac gagcgccttc
     2941 gcagcgctgg actgaccgat gggagcgcga actcgtccgg cgttgcctcc tcgcgccgcg
     3001 aatgagggca ggcacgaccg cttagaatcg cctccgccgt ccaaccgatg acactttcag
     3061 ccggaacccg tctcggttct tacgaaatcc tgtcgccgct cggagcgggc gggatggggg
     3121 aggtctacaa ggcgcgggac gggaagctcg atcgcgacgt ggcgatcaaa gttctgccgt
     3181 tctccgtcgc cggcgatcct gacgcgctcg ctcggttcga gagggaggcg aaggcggtgg
     3241 ccgcgctctc tcacccgaac attcttgcga tccacgactt cggaatgcac gacggcaccg
     3301 cctatgccgt gatggagctc ctggagggcg aaacgctggg cgcaaagctc gccgccggcc
     3361 caatcccacc caagcaggcg gtggactacg ccctgcaggt cgcccggggt ctttccgccg
     3421 cccacgagaa ggggatcgtc caccgggacc tgaagcccga caacatcttc gtcacgaaag
     3481 accgccacgt caagattctc gacttcggcc tggcaaggcg cgtcgagctg ttctccaacg
     3541 acggggagac gagcaatccc accgtatcca gccacacgca gccgggaacc gtgatgggga
     3601 cgatgggcta catgtcgccg gagcaggttc gcggctttcc cgcggaccat cgctcggaca
     3661 tcttttcctt cggggcgatc ctgtacgaac tcttgaccgg cgagaaggcg ttcaagaagg
     3721 acaccgggag cgacaccatg gccgcgatcc tgcgagacga gccgccgaag ccggcggatt
     3781 cgggcagaga gctttcgccc ggcctggaca acatcgtccg gcattgcctg gagaaggacc
     3841 gcgaccaccg ctttcagtcc gccagggaaa tcgttttcgc gctttcggag ggctcgacgc
     3901 tcacgtttcc gagcggcgtg cgattcgtgg agcggccgcc agcccgcaag cgcaaggcca
     3961 tctccatcac tgctgctgcg atccttcttc ttgccgtcgc gggagtcttt ctctggcgga
     4021 ggtcccctcc gagctccggc gaagtcgcgg ccgtcaagcg ggtggccgtg ctgccgttcg
     4081 agaacctcgg cgcggccgaa gacgactact tcgcggacgg gatcgccgat gagatccgta
     4141 gcaagctgac gtcgctgccg ggagtggagg tgatcgcgcg cggcagctcg acgccttaca
     4201 ggaagacgag caagaccccc agtcaaatcg cccgggagct cggagtcggg tacatcctga
     4261 cggccaccgt ccgatgggaa aagagcgaag gaagcggccg cgtccatgtc acccccgagt
     4321 tcgtcgaggt gaagaaggcc ggcgctccca cggcgaagtg gcagcagggg ttcgacgcgg
     4381 cgctgacgga cgtctttcag gttcagtcgg ccatcgccac gcgagttgct caggcgctgg
     4441 gggtcgcgct cggggcgggc gaggagaagc ggctttcgga gaagccgact caaaacctgg
     4501 ccgcctacaa cgcctacttg aaaggcgagg agatctggaa cggaacgggt gacgacgctc
     4561 ccagtgttcg aaaagcgctc gccttctacg atcaggctgt ggcgctggat cccgcttttc
     4621 tgcaggcgtg ggcgcgggtc tcctgggcca gctcgatcct gtacgccaac agcacgccca
     4681 cgcccgcgct cgcggagcgt gcccggtcgg cggccgaaaa ggccatcgtg ctcgcaccca
     4741 accgcccgga ggggtacctg gcgttcggcc acttcgaacg gctggtttcc aaggacttca
     4801 accgcgcgct ggagcagtac gcgaaggggc agcacgtcgc gcccggcaac gccgatctcc
     4861 tttatggaac ggcgcgtgcc gaggaaggcc tcgggcgctg ggatgcggcg gtggaccgct
     4921 tgaggcaggc ggcgagcgtc gatccccggt cggttgtcaa tttgacggct ctcggctacg
     4981 cgctccaacg tctccggcgc tatcccgaag cgcgggaatt ctacgagcgc ggtcttgcac
     5041 tcgcgccggc caatctgagc ctgatcgggg agacggccat gacgtttctg ggcgagggga
     5101 acctcgtcgg cgccagggcc gtgctcagcg ccgtgcccaa ggaggtcggg tcaaccgaac
     5161 tcgtggccta tatcgcgacc gccaacgatc tcgtctgggt tctcgacgaa cagcagcgag
     5221 aacttctcct gcggctcacg ccgagcgcgt tcgacgacga ccggggcaca tgggcccgtt
     5281 gcttgagtca ggcgtccgcc ctcaaaggcg acactgcgaa cgtccgcgcc tacgcggagg
     5341 aggcccggca ggcattcgag gagcagcttc gcgcagcacc ggacaatgcg ca
//
LOCUS       DASWAV010000152         2660 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U2665
            k141_449277, whole genome shotgun sequence.
ACCESSION   DASWAV010000152 DASWAV010000000
VERSION     DASWAV010000152.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960316
            Sequence Read Archive: SRR10260007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2660)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2660)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:47:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,929
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2660
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_449277"
                     /isolate="SMAG_U2665"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.36444 N 132.31889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..343
                     /locus_tag="VF958_03030"
     CDS             <1..343
                     /locus_tag="VF958_03030"
                     /inference="COORDINATES: protein motif:HMM:NF024748.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008800 - beta-lactamase activity
                     [Evidence IEA];
                     GO_process: GO:0030655 - beta-lactam antibiotic catabolic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HEX9148112.1"
                     /translation="FSRVARGELLDADSSARMFLYLDNSPSRARIARRFPSSALWAGK
                     TGTMSGVRNDAGILRTSKGRFVLVVFTNGSPGAGDNGQTHPAVVAMGDIARAIVDEWS
                     RGLPEVPGQPK"
     gene            378..1121
                     /gene="fabG"
                     /locus_tag="VF958_03035"
     CDS             378..1121
                     /gene="fabG"
                     /locus_tag="VF958_03035"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005035360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004316 - 3-oxoacyl-[acyl-carrier-protein]
                     reductase (NADPH) activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] reductase"
                     /protein_id="HEX9148113.1"
                     /translation="MNRFEGRVALVTGASRGIGEAIAQRLAAEGATILAAARSTPSLD
                     RVVAEISGAGGRARVIALDVSDASSIEAAMKSILEEHGQIDVLVNNAGISEDNLILRM
                     SREAWDRVIATNLTGAFLLTQAVVKGMVRRRYGRIVNVTSVVGLMGNAGQANYAAAKA
                     GLIGLTRSVARELGSRNITCNAVAPGFIATAMTDRMTDQARETLSVQIPVARLGRPED
                     VAGAVAFLASEEAGYVTGTVLNVSGGLYM"
     gene            1172..1411
                     /locus_tag="VF958_03040"
     CDS             1172..1411
                     /locus_tag="VF958_03040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018048122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HEX9148114.1"
                     /translation="MAVEEKVKSIIVEQLGVDANEVTPEASFVDDLGADSLDTVELVM
                     AFEEAFNIEIPDEAAEKIQKVKDAVEYIEKNSPQN"
     gene            1419..>2660
                     /gene="fabF"
                     /locus_tag="VF958_03045"
     CDS             1419..>2660
                     /gene="fabF"
                     /locus_tag="VF958_03045"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009856506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HEX9148115.1"
                     /translation="MPRFSSPRRVAVTGLGLVSPLGIGNGENWNALLAGRSGVGPITR
                     FDASGFACRIAGEVKGFDPSLYVEKKEIKKMDTFIHYAMAAAQFAMKDSSLPVTDGNR
                     ERIAVVIGSGIGGLPIIEETQKNYLQRGPRVISPFFITALIANEAAGNVSIRYGLKGP
                     NLATVTACTTGAHAVGEAYRMIQYGDADAAIAGGTESVITPLAVGGFAVMRALSTRND
                     EPERASRPWDRDRDGFVMGEGSGLVVLEEMEAAKKRGARIYAELAGYGLSGDAFHIAA
                     PSEDGDGPARVMRNALADAGISADQVGYINAHGTSTPLGDKVETIAIKMVFGDHAKKV
                     AVSSTKSSTGHLLGAAGGVETAICALALHHGVLPPTINYETPDPECDLDYVPNVGRDA
                     TLKFAMSNNFGLGGQNASVIFG"
BASE COUNT          466 a          873 c          902 g          419 t
ORIGIN      
        1 gttctcgcgg gtcgcccgcg gagagctcct cgacgcggac tcctcggcgc gaatgttcct
       61 ctacctcgac aactcgccgt cccgcgcccg catcgcgcgg cgcttcccct cctccgcgct
      121 gtgggcgggc aagaccggaa cgatgtccgg cgtgcgcaac gacgcaggga tcctgcgcac
      181 gtccaaaggc cggttcgtgc tggtggtctt caccaacggc agcccgggcg cgggcgacaa
      241 cgggcagacg cacccggccg tcgtcgcgat gggcgacatc gcgcgggcga tcgtggacga
      301 gtggagccgg ggtcttccgg aagtcccggg acaaccgaaa taggccttcg gacctccgac
      361 gatagactcg catgccgatg aatcgtttcg aagggcgggt tgcgctggtc accggcgcct
      421 cgcggggaat cggcgaggcg atcgcgcagc ggctcgctgc cgagggggcg acgattctcg
      481 ccgcggcgcg gtcgacgccc tcgctcgacc gcgtcgtcgc ggagatctcc ggcgcgggag
      541 gccgcgcgcg ggtgatcgcg ctcgacgttt ccgacgcctc ctccatcgag gcggcgatga
      601 agagcatcct cgaggagcat ggccagatcg acgtcctggt caacaacgcg gggatctcgg
      661 aggacaacct gattctgcgg atgtcgcggg aagcatggga ccgcgtcatc gcgacgaacc
      721 tgaccggggc ttttctcctc acccaggccg tcgtcaaggg gatggtgcgg cgccgctatg
      781 gccggatcgt caacgtcacg tcggtcgtcg gcctgatggg caacgccgga caggcgaact
      841 acgctgccgc caaagcgggt ctgatcgggc tcacccgttc ggtcgcgcgg gagctcggat
      901 cgagaaacat cacgtgcaac gcggtggcac ccgggttcat cgcgacggcc atgaccgacc
      961 gcatgaccga ccaggcgcgc gagacgctgt ccgttcagat cccggtcgcc cgcctgggcc
     1021 ggcccgaaga cgtcgcgggc gcggtggcat tcctcgcgtc ggaggaggcc ggctacgtca
     1081 cggggacggt cttgaacgtc agcggtgggc tttatatgta ggctgctttt ccgtctagaa
     1141 taccggtccg ctcgccgaat aaggaggatg gatggctgtc gaagaaaaag tgaagagcat
     1201 catcgtcgag caactcggcg tcgatgccaa cgaagtgacc cccgaggctt ctttcgtgga
     1261 cgacctgggc gcagattctc tcgacaccgt cgagctcgtg atggcattcg aggaggcctt
     1321 caacatcgag atcccggacg aggccgcgga aaagatccag aaggtcaagg acgccgtcga
     1381 gtacatcgag aagaactccc cgcaaaactg acacttcaat gcctcgcttc tcctctccgc
     1441 gccgggtggc cgtgacgggg ttgggactcg tatcccctct cggaatcggc aacggcgaaa
     1501 actggaacgc gctcctcgcc gggcgcagcg gcgtgggacc gattactcgc ttcgatgcct
     1561 cgggattcgc gtgtcggatc gctggcgagg tgaagggctt cgatccttct ctctacgtgg
     1621 agaagaagga gatcaagaag atggacacct tcattcacta cgcgatggcg gccgcgcagt
     1681 tcgcgatgaa ggactcgtcg cttcccgtca cggacgggaa ccgggagcgg atcgccgtcg
     1741 tgatcgggtc ggggatcggc ggccttccca tcatcgaaga gacccagaag aactacctgc
     1801 aacgcggacc ccgcgtgatc tcgcccttct tcatcacggc cctgatcgcg aacgaagccg
     1861 cgggaaacgt ctcgatcagg tacggcctca agggtccgaa tctcgcgacc gtaaccgcct
     1921 gcacgaccgg cgcgcatgcg gtcggcgagg cctaccggat gatccagtac ggcgacgcgg
     1981 acgccgcgat cgccggcggc acggagtcgg tgatcacgcc gctcgcggtc ggaggcttcg
     2041 ccgtgatgcg cgcgctctcg acccgcaacg acgagccgga gcgcgcctcc cgcccgtggg
     2101 accgcgaccg tgacggattc gtgatgggcg agggctcggg gctcgtggtg ctcgaagaga
     2161 tggaggcggc gaagaagcgc ggcgcgcgca tctacgccga gctcgccgga tacggtctct
     2221 cgggcgacgc cttccacatc gccgcgccgt cggaggacgg cgacggcccc gcgcgcgtca
     2281 tgaggaacgc gctcgccgac gccgggatct cggccgatca ggtcgggtac atcaacgcgc
     2341 acggcacctc cacgccgctc ggtgacaagg tggagacgat cgcgatcaag atggtcttcg
     2401 gcgaccatgc gaagaaggtc gccgtctcgt ccacgaaatc gtccacgggg cacctgctcg
     2461 gcgccgcggg gggggtcgaa accgccattt gcgcgctcgc cctccaccac ggcgtcctcc
     2521 cgcccacgat caactacgag acccccgatc ccgagtgcga tcttgactac gtgcccaacg
     2581 tgggccgcga cgccacgctg aagttcgcca tgtccaataa cttcgggctg ggcgggcaga
     2641 acgcgagcgt catcttcggg
//
LOCUS       DASWAV010000153         3797 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U2665
            k141_169441, whole genome shotgun sequence.
ACCESSION   DASWAV010000153 DASWAV010000000
VERSION     DASWAV010000153.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960316
            Sequence Read Archive: SRR10260007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 3797)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3797)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:47:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,929
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3797
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_169441"
                     /isolate="SMAG_U2665"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.36444 N 132.31889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1284)
                     /locus_tag="VF958_03050"
     CDS             complement(<1..1284)
                     /locus_tag="VF958_03050"
                     /inference="COORDINATES: protein motif:HMM:NF013106.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HEX9148116.1"
                     /translation="MTSPAAPPRHVSLPIWVARHRNEVILGAVAFLLLAIALVGFVES
                     AVASRFDSHRWNLPSRIFSDVAVFEPGAPGSADRLVAKLERLFYQASAGAPESPGHYR
                     RVGDAVEVYSRDFVYPGREFHGYRASVEFGGDTVRAIRDEHGDPLPALVVEPERLGAV
                     FGEEYEDRTLVRLRDVPRSLIDAILVTEDRDFYRHSGVSIKRTFGAILATLRGGTVQG
                     GSTLTQQLVKNLYLTPERTVRRKAVEATIAVLLEARYSKDEILEAYLNEIYLGQHGSI
                     AIKGVDEASRFYFGKQVADLDLAESATIAGMIKAPNSYSPFRSPQRTRERRDLVLGLM
                     LEQQKIDRAALDAAVASPLVPRSPRVQKIIAPHFVDFVKAQLSEKYGEKMRTEGLQIY
                     TTLDVDLQQAGQRAVTDGLASLEKRYRRLAAAARTA"
     gene            1283..2707
                     /locus_tag="VF958_03055"
     CDS             1283..2707
                     /locus_tag="VF958_03055"
                     /inference="COORDINATES: protein motif:HMM:NF016800.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HEX9148117.1"
                     /translation="MGRSAYRHNIDVLGGKKRRAHPPRSSGHSSRGRATPPPPAVPLT
                     LAQVAAIAPVVIVLAATALLLDPRAHASFDAPKRLAALVGASLAAFAALAFARPTRSL
                     RETWRTLPVAARVSVLAAAAALLWYGVGCALSPRRSASADTLRATVLLFLLVPVGATR
                     AIDRGKKILVAAFLGVTALNAAVSLLQSRGVQPFHLETFGTRNETGAFAGNVGYLSLT
                     VAFAAVLAIGIAIPAHRKTLRAGVAVLLLLLLATLVVNRNLTALAAVSAGTTALLVAR
                     FGRRSLLAIAVVGVVLLAAVLAIAPLRQRAADAVSAVRAGDWDSLTTYRTGAWAAAVE
                     MARERPITGWGPGTYAAEFVPHRLKAELRARRRFVNPLVTSSYSEAHCDYLQALAEAG
                     IPGGIAALVAAGALLAAVGRKAHEAGPSRIEAAILFGFLVAGAAAALTWFPLQRPLSA
                     VPLLLAAGRAWRLSAGWPEEAVPR"
     gene            2704..3228
                     /locus_tag="VF958_03060"
     CDS             2704..3228
                     /locus_tag="VF958_03060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9148118.1"
                     /translation="MRKVALAAAAIVLAALVWPEIGRYRAERQLGSANAAFELLLDRP
                     DEIRNAVEVLPQVSERAAAAAPSLPGDSRGWVLAGSCQLVAGKTDRALEFYRRALATG
                     ERAEIHLNLGRAEALLGRVEEAQAAFVRSVWISPALLRAVPPGFADPVTAEITRLESE
                     LRAGKLSAPPPPPQ"
     gene            complement(3200..>3797)
                     /locus_tag="VF958_03065"
     CDS             complement(3200..>3797)
                     /locus_tag="VF958_03065"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEX9148119.1"
                     /translation="HSANRGYGANQKTCYRTALERGADIVIMVHPDHQYDPRVIPDLL
                     ATLSSSGADAVFGSRMLGGRPIQGGMPKWKYLGNIFLTAVENATFLIYLTEYHSGFRA
                     YTRRYLETVNFEANSDGFVFDTEIIAQGMAKRLTIREIPIETRYFDEASQIAFGPSVR
                     YGLEILKTMGQYKLHEWGVWSSRIFRPSAATAEVEEAR"
BASE COUNT          545 a         1301 c         1311 g          640 t
ORIGIN      
        1 agccgtgcgg gctgcggccg cgaggcgccg gtaccgtttc tcgagcgacg ccaacccgtc
       61 cgtgacggcc ctctgcccgg cctgctgaag atccacgtcg agggtcgtgt agatctgcag
      121 cccctcggtt cgcatctttt cgccgtactt ttcggagagt tgcgccttga cgaaatccac
      181 gaagtggggc gcgatgatct tctggacgcg cggactgcgg ggcacgagcg gggatgccac
      241 cgcggcgtcc agcgcggcgc ggtcgatctt ctgctgctcg agcatgaggc cgaggacgag
      301 atcccgccgc tcgcgggtgc gctgcgggct gcgaaagggg gagtacgaat tgggcgcctt
      361 gatcattccg gcgatcgtcg cggactccgc caggtcgaga tccgctacct gcttgccgaa
      421 atagaaacgt gaagcttcgt cgacgccttt gatcgcaatc gagccgtgct ggccgaggta
      481 gatctcgttc agatacgcct cgagtatctc gtccttcgag taccgcgctt cgaggaggac
      541 cgcgatcgtc gcctcgaccg cctttcggcg caccgtgcgc tccggggtca ggtacaggtt
      601 cttcacgagc tgctgcgtca gcgtcgatcc gccctgcacg gtccctccgc gcagcgtggc
      661 gagaatcgcc ccgaaggtcc tcttgatcga gacgcccgag tgccggtaga agtcgcggtc
      721 ttcggtcacg aggatggcgt cgatgagaga tcgcgggacg tcgcgcaggc ggacgagcgt
      781 gcggtcctcg tactcctctc caaacaccgc gcccagccgc tcgggctcga cgacgagcgc
      841 ggggagggga tccccgtgtt cgtcccggat ggcgcggacg gtgtcgccgc cgaactcgac
      901 cgacgcgcgg taaccgtgga actcgcgccc cgggtagacg aagtctcggc tgtagacctc
      961 cacggcgtcg ccgacgcgcc ggtagtgtcc gggagattcg ggggcgccgg ccgacgcctg
     1021 gtagaagagt cgctcgagct tggcgacgag gcggtccgcg gaacccggag cccccggctc
     1081 gaagacggcc acgtccgaga agatccgcga cggaagattc cagcgatggc tgtcgaagcg
     1141 cgaggcgacc gccgactcca cgaatccgac gagcgcgatg gcgagaagga gaaacgccac
     1201 cgctccgaga atgacctcgt tccggtgtct cgccacccag atcggcagcg aaacgtggcg
     1261 cgggggcgcc gccggactgg tcatgggacg gtcagcatac cgtcataaca tcgacgtcct
     1321 gggcggaaag aaaaggagag cgcacccacc gcgaagctcg gggcactcga gccgcgggag
     1381 agccacgccg ccgccgccgg ccgttcccct caccctcgcg caggtcgccg caatcgcccc
     1441 cgtcgtgatc gtcctcgccg cgacggcgct cctcctcgat ccccgggctc acgcctcttt
     1501 cgatgcgccg aagcggctcg ccgcgctcgt gggggcgtcg ctcgccgcct tcgcagcgct
     1561 cgcgttcgcc cggccaactc gatcgctccg agaaacctgg cgcactctgc cggtggcggc
     1621 gcgcgtttcc gtcctcgcgg cggctgccgc cctcctctgg tatggggtcg gctgcgcgct
     1681 ttcgccccgc cgttcggcgt cggccgacac gcttcgcgcc acggttctcc tcttcctcct
     1741 ggtcccggtc ggcgccaccc gcgcgatcga cagaggcaag aagattctcg tcgcggcgtt
     1801 tctcggagtg acggcattga acgcggcggt ctcgctcctg cagagccgcg gggtccagcc
     1861 ctttcatctc gagacgttcg gcacgcgcaa cgaaaccgga gccttcgccg ggaacgtcgg
     1921 ttatctctcg ctgaccgtag ccttcgcggc ggttcttgcg atcggcatcg cgatcccagc
     1981 ccatcgaaag acgttacggg ccggcgtcgc cgtcctcctg ctcctgctgc tcgcgacgct
     2041 cgttgtcaat cgaaacctga ccgcgctcgc cgcagtgagc gccgggacga cggcgcttct
     2101 cgtggcgcgc ttcggccgcc gctctctcct cgcgatcgcg gtcgtcggcg tcgttctcct
     2161 cgccgcggtg ctcgcgatcg cgcccctgcg ccagcgggcc gctgacgcgg tgtcggcggt
     2221 tcgagccggc gactgggaca gcctgacgac gtatcgcacg ggcgcgtggg cggcggcggt
     2281 cgaaatggcc cgggaaagac cgatcaccgg ttggggtccc gggacctatg cggcggagtt
     2341 cgtgccccac cgattgaagg cggagcttcg cgcccgccgg cggttcgtca acccgctcgt
     2401 gacgagctcc tattcggaag ctcactgcga ctacctgcag gcgctggcag aggccggaat
     2461 tccggggggg atagccgccc tggtggcagc gggtgcgctc ctcgccgccg tcggccgcaa
     2521 agcgcacgag gccggtccct cgcggatcga ggcggcaatc ctcttcggct tcctcgtcgc
     2581 cggagcggcg gcggcgctca cgtggttccc tcttcagcgg ccgttgtccg cggttcctct
     2641 tcttctcgcc gccggccggg cctggaggct ctcggccggg tggcccgaag aggcggtccc
     2701 ccgatgagaa aggtggcgct cgccgcggcg gcgattgtgc tggccgccct ggtctggccg
     2761 gagatcggcc ggtatcgggc agagcggcag ctcggatcgg cgaacgccgc gttcgagctc
     2821 cttctcgacc gtcccgacga gatccggaac gccgtggagg tccttccgca ggtgagcgag
     2881 cgcgcggccg cggcggcgcc ctcactcccc ggcgactcgc gcggctgggt gctggccggg
     2941 tcgtgtcagc tcgttgcggg caagaccgac cgcgccctcg agttctaccg gcgggcgctc
     3001 gcgaccggcg agcgggccga aatccacctg aacctcggac gtgccgaagc gcttctcggc
     3061 cgggtcgagg aggcacaggc tgcattcgtc cggtcggtgt ggatcagtcc cgctctgttg
     3121 cgggcggtgc cacccgggtt cgccgacccg gtgacggcgg agatcacccg gttggaatcg
     3181 gagttgcgcg cggggaagct cagcgcgcct cctccacctc cgcagtagcg gccgagggcc
     3241 ggaagatccg gctcgaccac acgccccact cgtggagctt gtactgcccc atcgtcttca
     3301 ggatctcgag tccgtagcgg acggaggggc cgaaggcaat ctgcgacgcc tcgtcgaagt
     3361 aacgcgtctc gatcgggatc tctcggatgg tgagccgctt cgccattccc tgcgcgatga
     3421 tctcggtgtc gaagacgaac ccgtccgagt tcgcctcgaa gttcaccgtc tcgagatagc
     3481 gccgcgtgta cgcgcggaaa ccgctgtggt actcggtcag gtagatgaga aaggtcgcgt
     3541 tctcgacggc ggtcagaaag atgttgccga ggtacttcca cttgggcatg cccccctgga
     3601 tcggccgacc tcccagcatc cggctgccga acacggcgtc ggcgccggac gaggagagcg
     3661 tcgcaaggag atcggggatc acccgcgggt cgtactggtg gtccgggtgg accatgatga
     3721 cgatatccgc gccgcgttcg agcgccgttc gatagcaggt cttctggttc gcgccgtaac
     3781 cgcggttggc ggaatgg
//
LOCUS       DASWAV010000154         3917 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U2665
            k141_287923, whole genome shotgun sequence.
ACCESSION   DASWAV010000154 DASWAV010000000
VERSION     DASWAV010000154.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960316
            Sequence Read Archive: SRR10260007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 3917)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3917)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:47:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,929
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3917
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_287923"
                     /isolate="SMAG_U2665"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.36444 N 132.31889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..360
                     /locus_tag="VF958_03070"
     CDS             <1..360
                     /locus_tag="VF958_03070"
                     /inference="COORDINATES: protein motif:HMM:NF012321.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HEX9148120.1"
                     /translation="VVLSDGKDTASKFDYETLVEYVKKAGISIYGIGLKISGADLEVK
                     YKLNKLAQQTGGQTFYVDSAKNLESVYRQINDDLRSQYLLTYYSTNPSGKDKWRKVEI
                     KVEPSSMQARTITGYYP"
     gene            288..848
                     /locus_tag="VF958_03075"
                     /pseudo
     CDS             288..848
                     /locus_tag="VF958_03075"
                     /inference="COORDINATES: protein motif:HMM:TIGR00172.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphatase"
     gene            complement(856..2085)
                     /locus_tag="VF958_03080"
     CDS             complement(856..2085)
                     /locus_tag="VF958_03080"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HEX9148121.1"
                     /translation="MPVHQFSLLVQSAGTALLFIVFVLLYQKIRRRAFLDWIASWAFL
                     FASLTSLFLLPFLGQQWWFFFFLHACVLAHALLLLRGVRRFRDERLRSSRWELLWIVP
                     IVALSWWTSVEQGLVTRAAPVLLATAIAYAATGISFMVMRGSPGGRFLLSGSFLLWAT
                     ERAGLAYAIARFGAPDNMPGLLQYSAFLAMFLEMTIAVGIIILLFEASQTQLADEMHQ
                     LVSSDQELKEKGIRDPLTGLYNRHYFNDIIRRELASARRFGTSVSALLVDVDRFKEIN
                     DVKGHAVGDDVLRFVANYLTACVREADYVFRWGGDEFLIILTKTDGPSAAAKAEELVR
                     RLPNIPGTDNLQPTLSVGWASHRSDVEFPITLSEADSRMYEMKLARKRERADRERDRE
                     LLGEGDRRFEPAGPSRS"
     gene            2157..2915
                     /locus_tag="VF958_03085"
     CDS             2157..2915
                     /locus_tag="VF958_03085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9148122.1"
                     /translation="MRVHRARSGRVERPLRIALVAPTATAARAAAFVGASRVPELRLR
                     PFSFSRYASDLRAPLQVVLCPPGRSVEADLAFLREARRVFLWPPPAAEIWSAIAGLRG
                     SHEAPPTAAAPSPRAARRRAALLLEGDVTLDRARRAASSGAPREWIVERVQRVRISPK
                     GLGELRRLGVRWSVLEPVRVLAIAASSALIRARARWARLLPPDVAVWPLTSRPGSRGP
                     SNPATLPSGGTGNETQALAAAARRRPLRAAARRS"
     gene            complement(2855..>3917)
                     /locus_tag="VF958_03090"
     CDS             complement(2855..>3917)
                     /locus_tag="VF958_03090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020651826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="peroxidase family protein"
                     /protein_id="HEX9148123.1"
                     /translation="GELADAFARAWFKLLHRAMGPRSRYLGPWIPEPQLWQDPIPEVD
                     HELIGEQDVAALKRKILSSGLSIPELVSTAWSAAASFRGTDKRGGANGARIRLAPQKD
                     WESNEPTQLAKVLRTLEKIQQDFNGSQSGGKTVSLADLIVLGGCAAVEQAARNAGHDV
                     TVPFAPGRTDASQEQTDVDTFAVLEPTADGFRNYMRAGDPLSPETRLLDRSNLLKLTA
                     PEMTVLVGGMRALNANFGQSKHGVFTDRPETLTNDFFVNLLDIRTAWRPSASVENVYE
                     GRDRATGELKWTATAADLVFGAHSQLRALVEVYASDDAKEKFVRDFVTAWNKVMNLDR
                     YDLLAAARSGRRRAAAASA"
BASE COUNT          666 a         1301 c         1324 g          626 t
ORIGIN      
        1 gtcgtcctct ccgacggcaa ggacacggcg tccaagttcg actacgaaac gctcgtcgag
       61 tacgtgaaga aggcaggcat ctcgatctac ggcatcggcc tcaagatctc gggagccgat
      121 ctcgaggtga aatacaagct gaacaagctc gctcagcaga cgggcgggca gacgttctac
      181 gtcgactcgg cgaagaacct cgaatccgtg taccggcaga tcaacgacga cctgcgcagc
      241 cagtacctgc tcacctacta ctcgacgaat cccagcggca aggacaagtg gcggaaagtc
      301 gagatcaagg tcgagccgtc gagcatgcag gcacggacga tcacggggta ttacccgtaa
      361 cagacctcat cctcgcctcc tcctcccccc gtcgggccga gatcctgacg gcgctcggaa
      421 ttccgtttcg agtcagtccc gccgacgtcg acgaatcggt tctgccgggt gagacgggtc
      481 ccgccgcggc gtcgcgactc gcgggcgcga aggccgcctc cgcctccctc cgacatccgc
      541 gcgactgggt cctcgctgcc gacactctcg tgttgctcga cggcgccgtc ctcggcaagc
      601 cgcgggacga tggcgaagcg ggagagatgc tgcgaagcct gtcgggtcgg gagcaccgcg
      661 tcgtgaccgc cgtacggctg acacggggaa cgggccgcgg tcaggaagag gtcgaagaga
      721 gccgggtccg gatcgcgcct ctcgacgagg aagaaatccg gtggtacgtg tcgaccggag
      781 agcccctgga caaggcgggc gcctatgccg tgcaggggct cggggcgcgt ttcatcgagt
      841 cggtcgcggg ctccttcacg aacgtgatgg gcctgccggc tcgaagcgtc tatcgccttc
      901 tccgagcagc tcccgatccc gttctcggtc ggctcgctct cgcttccggg ccagtttcat
      961 ctcgtacatc cgggaatccg cctcggaaag ggtgatcgga aactccacgt cgcttctgtg
     1021 cgacgcccag ccgaccgaga gcgtcggctg gaggttgtcg gtcccgggaa tgttgggaag
     1081 acggcgcacg agctcctccg ccttggccgc agcggagggc ccatcggtct tggtcagaat
     1141 gatcaggaac tcgtctcctc cccaccggaa aacgtagtcc gcttcacgga cgcaggcggt
     1201 cagatagttg gcgacgaaac ggaggacgtc gtcgcccacg gcgtggccct tgacgtcgtt
     1261 gatttccttg aatcgatcga cgtcgacgag aagggccgag accgaggtgc cgaagcggcg
     1321 ggcgctcgcg agctcgcgtc ggatgatgtc gttgaagtag tgccggttgt agagaccggt
     1381 caacgggtcg cggatcccct tttccttcag ctcctggtcg gaggacacca gctggtgcat
     1441 ctcgtccgcg agctgcgtct gactcgcctc gaacagcaga atgatgatcc ccaccgcgat
     1501 cgtcatttcg aggaacatgg cgaggaacgc ggagtactgc agcagcccgg gcatattgtc
     1561 gggcgcgccg aaccgggcaa tcgcgtatgc gaggcccgcg cgttccgtcg cccacagcag
     1621 gaacgacccg gagagaagga accgccctcc tggcgatccg cgcatgacca tgaacgagat
     1681 cccggtggcg gcgtacgcaa tcgcggttgc gagaagaacg ggggccgcgc gggtgacgag
     1741 gccctgttcg acagacgtcc accaggagag cgcgacgatg ggaacgatcc agaggagttc
     1801 ccacctgctc gagcgcaggc gctcgtcccg gaatcgacgg acccccctca ggaggagcag
     1861 cgcgtgcgcg aggacgcagg cgtggagaaa gaagaagaac caccactgct gtccgagaaa
     1921 aggcagcagg aaaagggatg tcaggctcgc gaacaggaag gcccacgatg cgatccagtc
     1981 gaggaaagcg cgacgccgga tcttctggta gagcagaacg aagacgatga acagcagggc
     2041 tgtccccgcc gactggacca gcaacgaaaa ctggtgaacg ggcattcgat tctcggtcaa
     2101 cgagtaggac aatcttaacc cgctggaaag gaggccgagc cattcgcaat cgcccggtga
     2161 gagttcaccg cgcccgttcg ggccgcgtcg agcggccgct gcggatcgcc ctggtcgcgc
     2221 cgaccgccac cgccgcgcgc gcggcggcat tcgtcggcgc ctcccgggtg ccggaactcc
     2281 gcctccggcc cttctcgttc tcccgctacg cctccgatct ccgggcgccc ctccaggtcg
     2341 tcctctgtcc gcccgggcgg agcgtcgagg ctgacctcgc gtttcttcga gaagcgcggc
     2401 gcgtcttcct gtggccgccg ccggccgcag aaatctggag cgccatcgcg gggcttcggg
     2461 gatcccacga agctcccccg acggcggccg ctccatcgcc gcgggccgcg cgccgccggg
     2521 cagcgctcct cctcgagggg gacgtcacgc tggatcgcgc gcgtcgcgcg gcgtcctccg
     2581 gcgcgccgcg ggaatggatc gtggaacggg tgcagcgcgt ccggatctcg ccgaaaggcc
     2641 tcggcgagct caggcggctc ggcgtccgct ggagcgttct cgagccggtc cgggtgctcg
     2701 cgatcgccgc atccagcgcc ctcattcgcg cgcgagcacg ctgggcgagg ctgttgccgc
     2761 cggacgtggc ggtgtggcct ctgacgagcc gtcccgggtc ccgaggcccg agtaatcccg
     2821 ccactctccc ttcgggcggt accggtaacg aaactcaggc gctggccgcg gccgcgcgcc
     2881 gccgtccgct ccgggcggca gcgagaaggt cgtagcgatc gaggttcatg accttgttcc
     2941 aggcagtcac gaagtcacgc acgaacttct ccttcgcgtc gtcactcgcg tagacctcca
     3001 ccagcgcccg gagctgagaa tgtgcgccga acacgaggtc ggcggccgtg gcggtccact
     3061 tgagctcccc cgtggcgcga tcgcgaccct cgtacacgtt ctcaaccgag gccgatggtc
     3121 tccacgccgt gcggatgtcg agcaggttca cgaagaagtc gttggtcagc gtctcgggcc
     3181 ggtcggtgaa aacgccgtgc ttcgattgcc cgaagttggc gttcaaggcg cgcattccgc
     3241 cgacgagcac ggtcatctcg ggcgccgtca gcttcaacag gttggagcgg tccagcaggc
     3301 gcgtctccgg cgacagaggg tctcccgctc gcatgtagtt gcggaacccg tcggcggtcg
     3361 gttcgagcac cgcaaacgtg tccacgtcgg tctgctcctg cgaggcgtcc gtgcgacccg
     3421 gcgcgaacgg gacggtgacg tcgtgcccgg cgttcctggc cgcttgctcg acggcggcgc
     3481 atcccccgag gacgatcagg tcggccagcg agaccgtctt gccgccggac tgtgagccgt
     3541 tgaagtcctg ctggatcttc tcgagggtcc tcaacacctt cgcgagctga gtcggttcgt
     3601 tggactccca gtccttttga ggcgccaggc ggatccgtgc tccgttggcg ccgccccgct
     3661 tgtcggtgcc gcggaagctg gccgccgccg accaggcggt ggacacgagc tccggaatgg
     3721 acaggcccga ggagaggatc ttgcgcttca aggcggcgac gtcctgctcc ccgatcagct
     3781 cgtggtcgac ctccgggatg gggtcctgcc agagctgcgg ctccggaatc cacgggccga
     3841 ggtagcgcga ccgcgggccc atggcgcggt gaaggagctt gaaccacgcc ctggcgaacg
     3901 cgtcggcgag ctcaccc
//
LOCUS       DASWAV010000155        10368 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U2665
            k141_8695, whole genome shotgun sequence.
ACCESSION   DASWAV010000155 DASWAV010000000
VERSION     DASWAV010000155.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960316
            Sequence Read Archive: SRR10260007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 10368)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10368)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:47:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,929
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10368
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_8695"
                     /isolate="SMAG_U2665"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.36444 N 132.31889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1200
                     /locus_tag="VF958_03095"
     CDS             <1..1200
                     /locus_tag="VF958_03095"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HEX9148124.1"
                     /translation="FWGLGREGEVVRDLVPEFERRNPSVHLVVQQIPWTSAHEKLLTA
                     FVGRATPDLAQLGNTWIPEFAAIGALEPLDGSVARSTSVSSAGFFPGVWETSLYDGAL
                     YGIPWYVDTRVLFYRPDLLARAGFPSAPKTWSEWRDAMRKVKRLQPPAAHPILLPTNE
                     WEQPAILGLQKGSPLLAENGTRGAFAAPEFRAAAEFYVTIFREGLAPIVSYSALGNAY
                     QEFARGNIAMWMTGPWNLGEFRRRLTSAEQKLWMTAPLPAADGAPWPGVSFSGGSSLA
                     VFRGSEHKAEAWKVIEFLSDPAIQARFYALSGDLPASRAAWDDPALAGDEKARAFRVQ
                     VEHVAAMPRVPEWEQIAQDMAERLEPAIRGRETVPVALAATDRMVDRILEKRRWMLAR
                     RPEEHAR"
     gene            1190..2092
                     /locus_tag="VF958_03100"
     CDS             1190..2092
                     /locus_tag="VF958_03100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014503591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEX9148125.1"
                     /translation="MPAEGRRLPAEVRAAAIFLAPALLAIGLFFLLPIAASLLLSLTD
                     FDIYALGDLGNARFVGLRNFGRLLEDPLFWTSMRNTFYFVVVGGPLTLGVALAAALLL
                     HSKLARWKGLFRTIFFAPYVTTLVAVAIVFRYLYHPRFGGLNRLLEVFGIPAIDWLGD
                     PRWAMPAIILLAIWKNFGYAMILFIAGLQAIPESLYEAARIDGAGGFQVFRKITLPML
                     APTFLFLGVLTTIGYFQFFAEPYVMTGGGGPLNATLSTGLLMYKQGFRWWNMGYAAAI
                     AVVLGLVIFAFSALQFRFEKRRSS"
     gene            2089..2910
                     /locus_tag="VF958_03105"
     CDS             2089..2910
                     /locus_tag="VF958_03105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017461706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEX9148126.1"
                     /translation="MKRPTATIGLHLLLLLGAVAALAPLVWMVSASLMPTGEANSFPP
                     RFLPSRATLLHYRELFTRLDLGRAFASSLIVASATTLVSLLFNSMAGYAFAKLRFPGR
                     DRLFGTLIAAMVIPAPIAMLPLFLLLRSLGLVNSYAGVVLPAAATILGIFLIRQFARG
                     VPDDFIAAARLEGAGELEIYWRIVLPLLRPVLTTLGIVTFLATWNDFIWPLIILTDER
                     RYTLPVALASLVGEHVQDTELMMAGSVLTVLPVLLLFLLLQKQYIEGITLGGVKE"
     gene            2907..6062
                     /locus_tag="VF958_03110"
     CDS             2907..6062
                     /locus_tag="VF958_03110"
                     /inference="COORDINATES: protein motif:HMM:NF036578.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="discoidin domain-containing protein"
                     /protein_id="HEX9148127.1"
                     /translation="MRRLAPAALAGATLLLAAPPTDPARRVLDDFDDTARWTAAPSDG
                     VRLALSSDAGESSKALRMDFDFAGHAGWAAIRRDLPIRLPENWAIDLRVKGDTRPQTL
                     EFKLLDRSGQNVWWSVRRPYDFPRDWTTLRIRRRQLTFAWGPAGGGEIRDPGFVEITV
                     TSATGGKGTVWLDELALEELPPELSRPPPPVATASSSVSGSGAADAVDADPTTAWHSD
                     PVAGESQELDLDLGRRREFGGLVLDWDDRDYPRRYRIEVSDDGRDWTVHKHVSGARGG
                     RAFLYMPDSESRFIRLKLSESAQGRGYGLHEVDVRPPEFAESPTAFLQNVAREAPRGT
                     WPRSFAGEALYWTLVGVDAGSDEGLLSEDGTLETGRGAFSLEPFLWTGGKLLGWAQAE
                     TSHALLRGDLPIPSVTRRHAGGLELEVTAFADGPASGSTLHARYRVRNRGQTTISAKL
                     FLAARPLQVNPPWQFLNVPGGFSPIRTIAREGSSSFRFDRRRVLASPSSFGATGFEAG
                     EISRWLILGVLPPTAAAKDADGFASAALEWDLEIPSGESRDAVIAVPLAEAIASSDAG
                     ADFDSLVSNTARSWEEKVARVDFDLPDEAAVFARALSSNVGWILINRDGPAIQPGSRS
                     YSRSWIRDGALTSAALLRLGHESVARDFLKWFAPYQFDDGKVPCCVDRRGADPVPEND
                     SHGELLFLAGEYYRFTGDWRTIEDVWPHLSRAASYIDKLRRQRRTPEYASPAKRLSFG
                     LLPESISHEGYSAHPVHSYWDDFWAVKGLSDATELASALGRKPEARRFAASRDELRAD
                     LHESIRRVIASRGIDFVPGSADLADFDATSTTIALSPGEEQSRLPSRELARTFEKYWE
                     SFRARRSGSDRQEAYTPYEWRTVGAFVRLGWRDRAKEAMDFLLAARRPPEWNQWAEVV
                     WRDPRAPKFIGDMPHGWVGSDFIRAFLDLFAYDRPADGALVLGAGVPAEWLRAGRGVS
                     MHGLRTRWGRLDLAMREGKEGLRVSISGDLRVPPGGFAVRPPLSAPPRRVTVDGRGVA
                     FSAGELVVREVPAEILFQRSR"
     gene            complement(5971..7410)
                     /locus_tag="VF958_03115"
     CDS             complement(5971..7410)
                     /locus_tag="VF958_03115"
                     /EC_number="3.2.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007512217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008422 - beta-glucosidase activity
                     [Evidence IEA];
                     GO_process: GO:0030245 - cellulose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GH1 family beta-glucosidase"
                     /protein_id="HEX9148128.1"
                     /translation="MLPKATSGPLFPEDFLWGAATSAYQIEGSPLADGAGPSNWHRFA
                     HTPGLVQNGDTGDVACDHYRRFRQDVALMADLGLKAYRFSVSWSRVLPSGRGKVNPRG
                     LAFYDRLVDALLRKGIQPFTTIFHWDLPAALEDRGGWLNPDVSDWFAEYAGVLFRALD
                     DRVRFWATLNEPWVVTDGGYLHGVLAPGHRSPFETAIASHNLLRAHAAAVARYRAHGR
                     HAIGLVVNLEPKHAASSSPRDRAAALRADAYMNRQYLDPVFRGRYPRELHAMFGEAWP
                     RPAPDVSGIALPLDFLGVNYYTRGIVRHDPEVPHLSGVRVRRPRRLHTETHWEVYPAG
                     LTETLLWVRRRYGDVPIYVTENGAAFHDPPHSEDLVPDTHRIRYLREHLRAAHRAIEA
                     GVNLRGYFVWSLLDNFEWSHGYSKRFGIVHVDYRTQRRTPKASAAFYAEVIRTNGAAL
                     NVSAGKESPRVPLARRARPRRTRRPSRRP"
     gene            7467..9110
                     /locus_tag="VF958_03120"
     CDS             7467..9110
                     /locus_tag="VF958_03120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009634280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ligase PGM1-related protein"
                     /protein_id="HEX9148129.1"
                     /translation="MIPKPPFSCDLSPERELEEFRRIQPRLAELWNLLSNREEQPYTS
                     VVIPSLTLDQRELEKLEGASHYEERLLFLLIRLRNPQARMVYVTSRPVHPLILDYYFE
                     LLAGIPASHARSRLTLLCAYDTSPRSLTEKILERPRLIQRIRYGIGDPTRAFLTVYNS
                     TPLERRLAVLLGIPLNGVDPDLQSLGTKSGSRRAFREAGVAMPDGFEGLRSKSDLVEA
                     LDGLRRRQPAIRKAVVKLDDSFSGEGNAIFRYPGESDRTALEDALSRLSCAVSSESAD
                     SYLEKFRTMGGIVEEFLDGNEARSPSCQIRIDPSGKVVLISTHDQLLGGPTGQLYLGC
                     RFPADDGYRRQIQDAGRRVGEVLAARGVVSRLAIDFVVRRGPGSTEWKPFAIEINLRV
                     GGTTHPFLALEFLTGGQLDPETGLFHSPSGLAKYYRSTDNLKSDAYRGLSPEDLLDIT
                     TINHLHYSHGTETGVLFHMIGAVSQFGKLGITVIGNSHEQVNVVYQRAIAVLDRETEY
                     GRHSTRAQEEVAEPPAEARSGRPEYRDTIVVPRVWPADS"
     gene            9176..9793
                     /locus_tag="VF958_03125"
     CDS             9176..9793
                     /locus_tag="VF958_03125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011485594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HEX9148130.1"
                     /translation="MARGEIFNSVTHLVGSVFAFAGLVALLVVAVRTGDPWKIGSFAV
                     YGIALVELYLVSTLYHAVRGRAKRVFRRLDHTSIYVLIAGTYTPFALVSLRGHWGYPL
                     LASVWILAAIGILQEFLVPRRIRGLSVSLYLLMGWLAVAVFFPLRDAIGSAGMAWLVA
                     GGLLYTTGVFFYLVDEKYAHSHGIFHLFVLAGSILHFFVVLFFVA"
     gene            9833..10306
                     /locus_tag="VF958_03130"
     CDS             9833..10306
                     /locus_tag="VF958_03130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9148131.1"
                     /translation="MMNKFAIAAVAVAILTSAAACQNDNNDVAGSRGSLAVLNVDAPS
                     FAQSGVEFGVEISATSVGVSNVLDGHVVITFEAPLIPRSLDASAGTSASISSSSVTWD
                     LGTLDSNTRSRLQVRTIATLASGEASRSARIRAELTARGITSGEAVATDFVTVTP"
BASE COUNT         1552 a         3542 c         3457 g         1817 t
ORIGIN      
        1 ttctggggcc tcggaaggga aggggaggtc gtccgcgacc tcgttcccga attcgagcga
       61 cgcaacccca gcgtgcatct cgtcgtccag cagatcccgt ggacgtcggc gcacgagaag
      121 ctgctgacgg cgttcgtcgg ccgcgcgact ccggatctcg cgcagctcgg caacacctgg
      181 atccccgagt tcgccgcgat cggcgcgctc gagccgctcg atgggagcgt cgcacggtcg
      241 acgtccgttt cctccgccgg cttctttccg ggggtgtggg agacgagcct ctacgacggc
      301 gcgctctatg ggattccctg gtacgtcgac acgcgggtcc tgttttaccg ccccgatctg
      361 ctggcccggg ccggcttccc gagcgcgccg aagacatgga gcgagtggcg cgacgccatg
      421 cggaaggtca aacggctgca accgccggcc gcgcatccca tcctgctacc gacgaacgag
      481 tgggagcagc ccgcgatcct cggcctccag aagggctcgc cgctcctcgc cgagaacggc
      541 acccgcggcg cgttcgcggc tccggagttc agagccgccg cagagttcta cgtcacgatc
      601 ttccgcgaag ggctggcgcc gatcgtcagc tactccgcgc tcggcaacgc ctaccaggaa
      661 ttcgcgcgtg ggaacatcgc gatgtggatg acgggtccgt ggaacctggg cgagttccgt
      721 cgccggctca cttccgcgga acagaagctc tggatgacgg cgccgctgcc agccgccgac
      781 ggcgccccgt ggccgggcgt ttcgttttcg ggcggatcga gcctcgccgt ttttcgaggg
      841 tccgagcaca aggcggaagc gtggaaggtc atcgagtttc tgtccgatcc cgcgatccag
      901 gcgcgtttct acgctctctc cggcgacctc cccgcgtcgc gggccgcctg ggacgatccc
      961 gcgctcgccg gcgacgaaaa ggcgcgcgcc tttcgggtgc aggtggagca cgtggcggcg
     1021 atgccgcgcg tgcccgagtg ggagcagatc gctcaggaca tggctgaacg cctcgaaccg
     1081 gccatccgcg gtcgcgaaac ggtcccggtg gcacttgcgg cgacggaccg aatggtcgat
     1141 cggatcctcg agaagcggcg ctggatgctc gcgcgccgac cggaggagca tgcccgctga
     1201 gggccggcgc cttccggccg aagtccgagc cgcggcgatc tttctcgcgc ccgctcttct
     1261 cgccatcggc ctgttcttcc tcctcccgat cgccgcttcg ctcctgcttt cgctgaccga
     1321 cttcgacatc tacgcgctcg gcgatctcgg aaatgcgcgc ttcgtcggtc tccgcaactt
     1381 cgggcgactg ctcgaagatc cactcttctg gacgtcgatg cgcaacacgt tctatttcgt
     1441 cgtcgtcgga ggacctctta cgctcggcgt ggctctggcg gcggcgcttc tcctccactc
     1501 gaagctcgcc cgctggaagg gtctctttcg gacaatattc ttcgcgccct acgtcacgac
     1561 gctcgtcgcc gtggcgatcg tcttccgcta cctctaccac ccacgcttcg gcggtctgaa
     1621 ccggttgctc gaggtattcg ggatcccggc aatcgactgg ctcggcgatc cccggtgggc
     1681 gatgccggcg atcatcctgc tggccatctg gaagaacttc ggttacgcga tgatcctctt
     1741 catcgcagga cttcaggcga tcccggagtc gctctacgag gcggcgcgca tcgacggcgc
     1801 cgggggtttt caggtcttcc ggaagatcac gctgccgatg ctcgcgccga cctttctctt
     1861 cctcggagtc ctgacgacga tcggctactt ccagttcttc gccgaaccgt acgtgatgac
     1921 gggcgggggc ggaccgttga acgcgacgct ctcgaccggg ctcctcatgt acaagcaggg
     1981 gttccgctgg tggaacatgg gctacgcggc ggcgatcgca gtggtcctcg gtctggtcat
     2041 tttcgccttc agcgccctcc agttccgctt cgagaagagg cggagctcat gaagagaccg
     2101 acggcgacga tcggccttca cctcttgctc ctgctgggag ccgtagcggc tctggcgccg
     2161 ctcgtgtgga tggtgtcggc gtcgctcatg ccgacagggg aggcgaactc gtttccgccg
     2221 cgtttcctgc cttcccgagc gacactcctg cactaccgcg agctcttcac gcgcctcgat
     2281 ctcgggcgcg ccttcgcgag cagcctgatc gtcgcctcgg cgacgacgct cgtgtcgctg
     2341 ctcttcaact cgatggccgg atacgccttc gcgaagctcc gctttcccgg ccgcgaccgg
     2401 ctcttcggca ctctcatcgc ggccatggtc atcccggcgc cgatcgccat gctgccgctc
     2461 ttccttctgc tgcggtcgct cggactcgtc aacagctacg cgggcgtcgt tctcccggcg
     2521 gccgcgacca tcctgggaat cttcctgatc cgtcagttcg cgcgcggagt cccggacgac
     2581 ttcatcgccg ccgctcgcct ggagggcgcc ggagagctcg agatctactg gaggatcgtc
     2641 ctgcccctgt tgcggccggt gctgaccacg cttggcatcg tgacattcct cgcgacctgg
     2701 aacgacttca tctggcccct gatcatcctg acggacgagc ggcgctatac cctgccggtc
     2761 gccctcgcaa gcctggtcgg cgaacacgtc caggacaccg agctcatgat ggcgggatcg
     2821 gtgctgaccg tcttgccggt tctgctcctc tttctcctcc tccagaagca gtacatcgag
     2881 gggatcacgc tcggaggtgt gaaggaatga ggcggctcgc gccggccgcc ctggcgggcg
     2941 cgacgctcct cctcgcggcg cccccgaccg atcccgcgcg ccgggtcctc gacgacttcg
     3001 acgacaccgc gcgctggacc gccgctccgt ccgacggtgt gcggctggcg ctctcctcag
     3061 acgcgggcga gagcagcaag gctctccgga tggacttcga cttcgccggc cacgcgggct
     3121 gggccgcgat ccgccgggac cttccgataa ggctgcccga gaactgggcg atcgatctgc
     3181 gcgtgaaggg cgacactcga ccgcagacgc tggagttcaa gctcctcgac cgttccgggc
     3241 agaacgtctg gtggtcggtg cgccggccgt acgactttcc gcgggactgg acgactcttc
     3301 gcatccgacg gcgccagctg acctttgcgt gggggcccgc agggggcggc gagatcaggg
     3361 acccgggttt cgtcgaaatc accgtgacct cggcgaccgg tgggaaggga acggtctggc
     3421 tcgacgagct cgcgctcgaa gagcttccgc ctgaactctc gaggccgcct ccgcctgttg
     3481 cgacggcatc gtcctctgtc tccggttcgg gcgccgccga cgcggtcgat gccgatccga
     3541 cgacggcctg gcacagcgac ccggtggcgg gcgaatccca ggagctcgat ctcgacctcg
     3601 gccggcgccg ggagttcggc ggactcgtcc tcgactggga cgaccgcgac tacccgcgtc
     3661 gctatcgcat cgaggtctcc gacgacggtc gggattggac cgtgcacaag catgtctccg
     3721 gcgcccgggg aggaagagca ttcctctaca tgcccgactc ggagtcgcga ttcatccgtc
     3781 tgaagctctc cgagagcgca cagggccgcg gatacgggct ccacgaagtc gacgttcgcc
     3841 ctccggagtt cgccgagagt ccgaccgcct ttctgcagaa cgtcgcccgc gaggcgccgc
     3901 gaggaacgtg gccgcgctcg ttcgctggag aagcgctgta ctggactctc gtcggcgtgg
     3961 acgcgggaag cgacgaagga ctgctgtccg aggacgggac cctggagaca ggacgcggag
     4021 cgttctcgct cgagccgttt ctctggaccg gcggaaagct cctcgggtgg gcgcaggcgg
     4081 aaacctcgca tgcgctcctg cgcggcgatc tgccgatccc gtccgtgacg cgacgtcacg
     4141 cgggcggctt ggagctcgag gtcacggcgt tcgccgacgg ccctgcctcg ggctcgacct
     4201 tgcacgcccg ataccgcgtt cgaaatcggg gacagaccac gatttccgcg aagctctttc
     4261 tcgccgcgcg gccgctccag gtcaatccgc cgtggcagtt cctgaacgtg ccgggtggct
     4321 tctcaccgat ccggaccatc gcgcgcgagg gctcgtcctc gttccgattc gaccgccggc
     4381 gagtgctcgc cagcccctcg agcttcgggg cgaccggctt cgaggctggg gaaatctcga
     4441 ggtggctgat cctgggagtc cttccgccga cggccgcggc gaaggacgcc gacggcttcg
     4501 cgtcggcggc gctcgagtgg gacctcgaga ttccttcggg cgaatcccgc gacgccgtca
     4561 tcgcggtgcc gctggcggag gcgatcgcga gctcggatgc cggcgccgac ttcgattctc
     4621 tcgtttccaa cacggcgcga tcgtgggagg aaaaggtcgc gcgcgtcgat ttcgatctgc
     4681 cggacgaggc cgcggttttc gcccgcgcgc tctcctccaa cgtcggctgg atcctcatca
     4741 atcgcgacgg ccctgcgatc cagccgggat cccgctcgta ctcgcggtcc tggatccggg
     4801 acggcgcgct gacgtcggcg gcgctccttc gcctcggtca cgagtccgtc gcgcgcgact
     4861 tcctgaagtg gttcgcgccg taccagttcg acgacggcaa ggtcccttgc tgcgtcgacc
     4921 gacgaggagc ggatccggtc cccgagaacg acagccacgg agagctcctc ttcctcgccg
     4981 gggagtacta ccggttcacg ggagattggc ggacgatcga agacgtctgg ccgcacctct
     5041 cccgcgccgc gtcgtacatc gacaagctgc ggcggcagcg ccggactccg gagtacgcct
     5101 ctcccgcgaa gcgactctct ttcgggctcc tccccgagtc gatcagccac gagggctatt
     5161 cggcgcatcc ggtccactcg tactgggacg acttctgggc ggtgaaagga ctttcggacg
     5221 cgacggagct cgcgagcgcg ctcgggcgga aaccggaggc gcgccgcttc gcggcctcgc
     5281 gcgacgagct tcgcgccgat ctgcacgagt cgatccggcg cgtgatcgct tcgcgtggga
     5341 tcgatttcgt tcccggctcg gccgacctcg ccgacttcga cgcgacctcg acgacgatcg
     5401 cgctgtcgcc gggagaggag cagtcgcgtc ttccttcgcg cgagctcgcg cgcacgttcg
     5461 agaagtactg ggagagcttc cgcgcgcggc ggtccggttc tgaccggcag gaggcctaca
     5521 ccccctacga gtggcggacg gtcggcgcct tcgtgcggct cggatggagg gatcgcgcga
     5581 aggaagccat ggacttcctg ctcgcggcac gccgccctcc cgaatggaac cagtgggccg
     5641 aggtcgtctg gcgcgacccg cgcgccccga agttcatcgg cgacatgccg cacggatggg
     5701 tcggatcgga tttcatccga gcgtttctcg atctcttcgc ctacgacagg cccgcggacg
     5761 gtgccctggt gctcggcgcc ggagtccccg cggagtggct ccgggccgga cgcggggtct
     5821 cgatgcacgg cctgcggacc cggtggggga gactcgacct cgcgatgcgc gaggggaagg
     5881 agggtcttcg agtgtcgatc tccggcgatc ttcgggtccc tcccggcggc ttcgccgtgc
     5941 gcccaccgct ctccgcgcct ccccggcggg tcacggtcga cgggaggggc gtcgcgttct
     6001 ccgcgggcga gctcgtcgtg cgagaggtac ccgcggagat tcttttccag cgctcacgtt
     6061 gagcgccgcg ccgttcgtgc ggatcacctc ggcgtagaag gcggcgctcg ccttcggcgt
     6121 gcgtctctgc gtccggtagt ccacgtgcac tatgccgaag cgcttggagt aaccgtggct
     6181 ccactcgaag ttgtcgagaa gcgaccagac gaagtatccg cgcagattca ctcccgcctc
     6241 gatcgctcgg tgcgctgccc gcaggtgctc gcgaaggtaa cggattcggt gcgtgtcggg
     6301 gacgaggtct tccgagtggg gtgggtcgtg gaacgccgcg ccgttctcgg tcacgtagat
     6361 cgggacatcg ccgtaacggc ggcgaaccca gaggagcgtc tcggtcaggc ccgccggata
     6421 gacctcccag tgagtctccg tgtgcaggcg ccgcggtcgg cggacgcgga cacccgagag
     6481 gtgcgggacc tccgggtcgt gccgcacgat cccgcgcgtg tagtagttca cgccgaggaa
     6541 atcgaggggg agcgcgatgc ccgagacgtc cggggcgggg cgcggccacg cctcgccgaa
     6601 catcgcgtga agctccctcg gataccggcc gcggaagacg ggatccaggt actggcggtt
     6661 catatacgcg tcggcgcgaa gcgccgcggc gcggtctcgc ggcgacgacg acgccgcgtg
     6721 cttcggttcg aggttgacga cgaggccgat cgcatgccgc ccgtgcgcac ggtaccgggc
     6781 gacggcggcc gcgtgcgccc gcagcagatt gtgtgaggcg atcgcggtct cgaaaggact
     6841 gcggtggccg ggcgcgagca cgccatggag gtagccgccg tcggtgacga cccacggctc
     6901 gttcagcgtc gcccagaacc gaacgcggtc atcgagcgcg cggaagagca ccccggcgta
     6961 ttcggcgaac cagtccgaga cgtccgggtt cagccagccg ccccggtcct cgagcgccgc
     7021 cggaaggtcc cagtgaaaga tggtcgtgaa gggctggatt cctttccgga ggagcgcatc
     7081 cacgagacgg tcgtagaacg cgagaccccg gggattgacc ttgccgcgtc cggagggaag
     7141 gacacggctc cacgaaacgc tgaaccggta cgccttcaag ccgaggtccg ccatgagcgc
     7201 gacgtcctgg cggaagcgcc ggtagtgatc gcacgcgacg tcgcccgtgt cgccgttctg
     7261 gaccaggccg ggagtgtgag cgaagcgatg ccagttgctc ggaccggcac cgtcggcgag
     7321 cggcgaacct tcgatctggt acgccgaagt cgccgctccc cacaggaagt cctcgggaaa
     7381 aaggggaccc gacgtggctt tgggaagcaa gactttcgct atcctcgcga agacagggaa
     7441 gggaccgcga caaccgagac accgtcatga taccgaagcc ccctttttcg tgcgacctgt
     7501 cgcctgagcg cgaactcgag gagtttcgca ggatccagcc ccgcctggcg gagctctgga
     7561 accttctgtc gaaccgggag gaacagccct atacgtccgt cgtcatcccc tctctcaccc
     7621 tcgaccagcg ggagctcgag aagctcgaag gcgcgagcca ctacgaggag cggcttctgt
     7681 tcctgttgat ccggctgcgg aacccccagg cccggatggt ctacgtcacg tcgcgtcccg
     7741 tccatcctct gatcctcgat tactacttcg agctcctcgc ggggatcccc gcgagccatg
     7801 cgcgctcgcg gttgacgctc ctctgcgcct acgacacgtc gccgcggtcg ctcaccgaga
     7861 agatcctcga gcggccgagg ctcatccagc ggatccggta cggcatcggc gaccccaccc
     7921 gcgcgtttct gaccgtctac aactcgactc cgctcgagcg acggctggcc gtgctcctcg
     7981 gcatccccct gaacggagtg gaccccgacc tccagtcgct cggaacgaaa tcgggcagcc
     8041 ggcgcgcgtt ccgcgaagcc ggcgtcgcga tgcccgacgg attcgaggga ctccggtcga
     8101 agagcgatct cgtcgaagcg ctcgacggac tgcggcggag gcagcccgcc atccgcaaag
     8161 cggtcgtgaa gctcgacgac agcttctcgg gcgaagggaa cgcaatcttc cgttaccccg
     8221 gggagagcga ccgtactgcg ctcgaggacg cgctttcccg cctgtcgtgc gccgtctcct
     8281 cggagtccgc cgattcgtat ctcgagaagt ttcgaacgat gggaggcatc gtcgaagagt
     8341 tcctggacgg gaacgaggcg cgatcgccga gctgccagat ccggatcgac ccgagcggca
     8401 aggtggtctt gatctccacg cacgaccagc tgctcggggg accgaccggt cagctctacc
     8461 tcggctgccg gtttccggcg gatgacgggt accgccgcca gatccaggac gcgggccgcc
     8521 gtgtgggtga ggtgctcgcc gcgcgcgggg tcgtcagccg cctcgcgatc gacttcgtcg
     8581 tccgccgcgg cccgggctcg acggagtgga agccattcgc gatcgagatc aaccttcggg
     8641 tcggcggcac gacgcacccc ttcctcgcgc tggagttcct gaccgggggg cagctcgacc
     8701 ccgaaacggg cctctttcac tcgccgagcg ggctcgccaa gtactatcgc tcgaccgaca
     8761 acctgaagtc cgatgcgtac cggggacttt cgcccgagga tctgctcgac atcacgacga
     8821 tcaaccacct ccattacagc cacggcaccg agaccggagt gctctttcac atgatcggcg
     8881 cggtctcgca gttcgggaag ctggggatca cggtgatcgg aaactcgcac gaacaggtga
     8941 acgtggtgta ccagagggcg atcgcggtcc tcgaccgcga aacggaatac gggcggcatt
     9001 cgacgcgcgc ccaagaggaa gtggccgagc ctcccgcaga ggcgcgctcc gggaggcccg
     9061 agtaccgcga cacgatcgtc gtgccgcgcg tgtggcccgc cgacagttga tcgcacgccg
     9121 gcggtgaccc gcggcggcgg ccgctgaaag gggcgatttc gacgtgcgag aactggtggc
     9181 ccgcggcgag atcttcaaca gcgtcaccca cctggtcgga tccgttttcg cgttcgccgg
     9241 cctcgtcgcg ctcctcgtgg tggcggtgcg gaccggggac ccctggaaga tcgggagctt
     9301 cgcggtttac ggaatcgcgc tggtcgagct ctatctcgtg tccaccctgt accatgccgt
     9361 ccggggccgg gcgaagcgcg tctttcggcg gctcgaccac acgagcatct acgtgctgat
     9421 cgcgggcacg tacacgccgt tcgcgctggt cagcctccgc ggacactggg gctatccgct
     9481 tctcgcctcg gtgtggatcc tggccgctat cgggattctc caggagtttc tggttccccg
     9541 tcggatccgc ggcctctccg tctccctgta tctcctcatg ggctggctcg cggtcgcggt
     9601 gttcttcccc ttgcgggacg cgatcggctc ggccggaatg gcctggctcg tcgcgggggg
     9661 ccttctttac acgaccggag tgttcttcta cctggtggac gagaagtacg cccactccca
     9721 cggcatcttc cacctgttcg tgctcgcggg gagcattctg cacttcttcg tcgttctgtt
     9781 cttcgtcgcc tgagtggcac cccgattgct gctctcggac acggaggccc tgatgatgaa
     9841 caagtttgca atcgccgcgg tggccgtcgc gatactgact tcggcggctg cctgccagaa
     9901 cgacaacaac gacgtcgccg ggtctcgagg ctccctcgcg gtcctgaacg tcgacgcgcc
     9961 gagcttcgcc cagtccggag tcgagttcgg agtcgagatc agtgcgacga gcgtcggcgt
    10021 ctcgaacgtc ctcgacggtc acgtcgtgat caccttcgaa gcgccgctga ttccgcgatc
    10081 gctggacgcg tccgcgggaa cgagcgcctc gatcagcagc agctccgtga cctgggacct
    10141 gggcacactc gattcgaata cccgctcacg ccttcaggtc cggaccatcg cgaccctggc
    10201 atccggtgaa gccagccggt ccgcgaggat ccgtgcggag ctgacggcgc gcggaatcac
    10261 gtccggcgag gcggttgcga cggatttcgt gacggtaacg ccttagctca ccgggggctc
    10321 ggcgactcac ttgaaggcga gcgcgacgtt cgcgaagact cgccactc
//
LOCUS       DASWAV010000156         2047 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U2665
            k141_449359, whole genome shotgun sequence.
ACCESSION   DASWAV010000156 DASWAV010000000
VERSION     DASWAV010000156.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960316
            Sequence Read Archive: SRR10260007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2047)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2047)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:47:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,929
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2047
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_449359"
                     /isolate="SMAG_U2665"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.36444 N 132.31889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..232
                     /locus_tag="VF958_03135"
     CDS             <1..232
                     /locus_tag="VF958_03135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012909548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="translation initiation factor"
                     /protein_id="HEX9148132.1"
                     /translation="PKISAKLSIEKRGSGSVVTVVGGLPANRGFLDALARELKKSCGA
                     GGRAGETSVEIQGDHRERLHDLLARKGWTVKG"
     gene            428..1750
                     /locus_tag="VF958_03140"
     CDS             428..1750
                     /locus_tag="VF958_03140"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015895553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEX9148133.1"
                     /translation="MPFTSFDLHPDILKGVRELGFTRPTPIQTDALPPALAGRDLLAS
                     AETGSGKTAVFVLAILQRLLPKPRRATRALVITPTRELAAQIEEHVRQLAVHTPIQTA
                     SVFGGVAMGPQEHAFRAGVDVLVATPGRLLDHFRHGYARLGSLEVLVLDEADRMLDMG
                     FLPDVRRILKHLPAHRQTLFFSATMPPPIAALAREILRNPVSVNIERRAAPAVGITQA
                     VYPVTGNLKTPLLVELLRRGDLENALVFTRTKHRANRLYEALAKRGVSCERIHGNRSQ
                     PQRTQALAGFKSGRYRVLVATDIAARGIDVEALPHVVNFDVPHVPEDYIHRVGRTARA
                     ERTGDAFTFVSPEEEADLRGIEKALGRRLPRITVPGFDYSAAPAERFEIPVGERIAAI
                     RARKSEERARAKAKGQRRAASVAPSSPRAEAPSFGRGPRPRPIRKRRG"
BASE COUNT          320 a          732 c          710 g          285 t
ORIGIN      
        1 gccgaagatc tcggcgaagc tctcgatcga gaagcgcgga tcgggaagcg tcgtcaccgt
       61 ggtgggaggg cttcccgcca accgcggctt cctcgacgcg ctcgcgcggg agctcaagaa
      121 atcgtgcggc gccggggggc gcgccggaga aacgtccgtc gaaatccagg gcgaccaccg
      181 ggagcggctg cacgacctgc tcgcgcggaa ggggtggacc gtcaagggtt gacccggtct
      241 tcggttcgcg gcgcgaggag tctcggcgcg cgcttgcgcc ggggcggcga aacgtgacac
      301 cctgaagggt ccccgcgcca ggccggcgcg acgataacgg cggtctgccg gcgacccctc
      361 ccgttctctc ccgaaaaaca cgccggaaac atggcggggc cacgccccgc ggaaagattc
      421 ttgcctcatg ccttttacga gcttcgacct tcaccccgac atcttgaagg gcgtccgcga
      481 actggggttc acgcgcccga ccccgatcca gacggatgcg ctcccccccg cgctcgccgg
      541 acgggacttg ctcgccagcg ccgaaaccgg cagcggaaag actgccgtat tcgtcctcgc
      601 gatcctgcag cgtctcctgc cgaaacctcg gcgggcgacc cgggcgctcg tcatcacccc
      661 gacgcgcgag ctcgccgcgc agatcgaaga gcacgtccgc cagctcgcgg tgcacacgcc
      721 gatccagacg gcgtcggtct tcggcggggt cgcgatggga ccgcaggagc acgcgttccg
      781 cgcgggcgtc gacgtcctgg tggcgacgcc ggggcgcctc ctcgaccact ttcgccacgg
      841 ctatgcgcgt ctcgggagcc tcgaggtgct cgtgctcgac gaggcggacc gcatgctcga
      901 catggggttc ctccccgacg tgcgccggat cctgaagcac ctgccggccc atcgccagac
      961 cctcttcttt tcggcgacga tgccgccgcc gatcgcggcc ctcgcgcgcg agatcctccg
     1021 caacccggtg tcggtgaaca tcgagcggcg cgccgccccc gcggtcggga tcacgcaggc
     1081 ggtctacccc gtcacgggga atctcaagac gccgctgctc gtggaattgc tccgccgcgg
     1141 cgacctggag aacgccctgg tcttcacccg cacgaagcat cgcgccaacc ggctctacga
     1201 agcgctggcg aagaggggcg tgtcgtgcga gcgaatccac ggcaatcgga gtcagcctca
     1261 gcgcacgcag gctctggcgg ggttcaagag cggacggtat cgcgtcctgg tcgcgaccga
     1321 catcgccgcc cgcggcatcg acgtcgaggc gctgccccac gtcgtcaact tcgacgtccc
     1381 gcacgtgccc gaggactaca tccaccgggt cggccgaacc gcgcgagcgg agaggacggg
     1441 cgacgcgttc accttcgttt ccccggagga ggaggccgac ctgcgcggaa tcgaaaaggc
     1501 gctcggtcgc cggctcccgc ggatcaccgt ccccggtttc gattactccg cggcgcccgc
     1561 cgagcgcttc gagattcccg tcggcgaacg gatcgccgcg atccgggcgc gcaagtcgga
     1621 ggaacgcgcc cgggcgaagg cgaagggtca acgccgggcc gcctccgtgg cgccgtcgtc
     1681 accgcgggcg gaagcgcctt ctttcggtcg cggcccgcga ccgcgtccaa tccggaaacg
     1741 gcggggctga atctcgccgg attcagcgcg cgccagtcga aagtcgaggt ctgtccccgt
     1801 tttcgcggcg cgaggagcct cccgaggtag tcgagggagg agcttctcgc cgcaggagcg
     1861 cgagcgaagc gagggatcag ggctcggtcg agcccggtat ggcgagccga gcgccggtgg
     1921 cgtcagccag atcccgagca agcgaaggcg acgaagttac cttgctccgc cgccgtaaac
     1981 cggcagcgcg acaccgaccc cggttgcggc cgggcgcgcg ggttgcgtcg ccggcaacgg
     2041 ctgtccg
//
LOCUS       DASWAV010000157         1752 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U2665
            k141_648381, whole genome shotgun sequence.
ACCESSION   DASWAV010000157 DASWAV010000000
VERSION     DASWAV010000157.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960316
            Sequence Read Archive: SRR10260007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 1752)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1752)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:47:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,929
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1752
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_648381"
                     /isolate="SMAG_U2665"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.36444 N 132.31889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1752)
                     /locus_tag="VF958_03145"
     CDS             complement(<1..>1752)
                     /locus_tag="VF958_03145"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013927.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016811 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear amides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin acylase family protein"
                     /protein_id="HEX9148134.1"
                     /translation="FEPVDALAWAKLMAWDLAGNARTEIRRARFAAAVGEKGAAELLP
                     PVPETPTILLDAEWASSRLPLPPGEGRGEGGTSAGALSPKLLAKLDGLFALAGARDGD
                     AGVGSNSWVLAGSRTLSGKPILANDPHLGLRAPSVWYLARLVAPGYSVAGATLPGVAG
                     VVIGANARIAWALTSLEPDVQDLFVEELDPADSSRYLWRGASRAFETRRETIRVRGSG
                     DVVVNVRRSVHGPIVTDVFDGAASLGRAVALRWAALDEADSTAQAIEGINRASNWPQF
                     LEAVRHFHAPPQNFLYADADGHIGYTASGSIPIRPRADGLLPVSGSGDDDWRGYVPFD
                     ALPRSLDPPRGFLVTANNRVVSPRYPYPIARDWPEPYRARRITDRILGKGRLDIGDVR
                     SIQLDRVSLQATELLPLLSATAPADDASRKALSTLSAWNREFAPDSAAAAIYAAWYAE
                     LARMPEDELGSTPPGAVRSRFLIDALSSESKWCDDVRTPARESCGEFRTKTLREAVSL
                     LRKRLGTDPGRWRWEDLHHARFPHGVFEAVPGLRSIFGLETGQGGDASTVNVGAYRLD
                     GSFRMTDGPSYRQIIDFS"
BASE COUNT          289 a          604 c          627 g          232 t
ORIGIN      
        1 cgaaaagtcg atgatctggc gatagctcgg accatcggtc atccggaagg aaccgtccag
       61 acgataggcg ccgacgttga ccgtcgaggc gtcgcctccc tgccccgtct cgagaccgaa
      121 gatcgagcgc agtccgggca cggcctcgaa aactccgtgc ggaaagcgcg cgtggtggag
      181 gtcttcccag cgccaccgtc ccgggtccgt cccgaggcgc ttgcgcagaa gagacacggc
      241 ctcccttagc gtcttcgtcc gaaactctcc gcagctctcc cgagcgggcg tccgcacgtc
      301 gtcgcaccac ttcgactccg aggacagcgc atcgatcagg aaacgcgacc ggaccgcgcc
      361 gggcggcgtc gacccgagct cgtcctcggg catccgggcg agctccgcgt accaggcggc
      421 gtagatcgcg gcggccgccg agtccggcgc gaactcgcga ttccaggccg agagcgtcga
      481 gagcgccttg cgggacgcgt cgtccgccgg cgccgtcgcc gagagcagcg gcaggagctc
      541 ggtcgcctgg agagagacgc ggtccagctg gatcgagcgg acgtcgccga tatcgagcct
      601 tcccttcccg aggatccggt ccgtgatccg tcgcgcgcgg tacggctccg gccagtctcg
      661 agcgatcggg tacgggtaac ggggcgagac gacgcggttg ttcgccgtga cgaggaagcc
      721 gcggggggga tcgagcgagc gcgggagcgc atcgaacggg acgtagccgc gccaatcgtc
      781 gtcgccggag ccggagaccg gaagaaggcc gtccgctcga ggccggatcg ggatcgaccc
      841 ggacgccgtg tagccgatgt ggccgtccgc atcggcgtag aggaagttct ggggcggtgc
      901 atggaagtgg cgcaccgctt ccaggaactg cggccagttc gaggcgcggt tgattccctc
      961 gattgcctgc gcggtcgagt cggcctcgtc gagcgccgcc cagcggagcg ccacggcgcg
     1021 gccgagggaa gccgccccat cgaagacgtc ggtcacgatc ggaccgtgca cggagcggcg
     1081 cacgttcacg acgacgtcgc cgctgcccct cacgcggatc gtctcgcgcc gcgtctcgaa
     1141 tgcgcgggag gcgccgcgcc acaggtagcg ggaggaatcg gcgggatcga gctcctcgac
     1201 gaacaggtcc tgcacgtcgg gttcgaggct cgtcagcgcc cacgcgatgc gcgcgttggc
     1261 gccgatcacg acgccggcaa cgcccggcag cgtcgctccg gccacggagt agcccggcgc
     1321 gacgaggcgc gcgaggtacc acaccgaagg agcgcgcaag cccaggtggg gatcgttcgc
     1381 gaggatgggc ttccccgaca aggtgcgcga gccggcgagg acccaggagt tgctgccgac
     1441 acctgcgtcg ccgtcgcgag caccggcgag tgcaaagaga ccgtccagct tcgcaagcag
     1501 cttcggagag agcgctcctg ctgaagttcc cccctcaccc cgaccttctc ccggaggcaa
     1561 agggagccgg ctcgacgccc attcggcgtc gagcagaatc gtcggcgtct cggggaccgg
     1621 cggcaggagc tccgcagcgc ctttctcacc gaccgcagcg gcaaacctgg cgcgccggat
     1681 ctccgtgcgc gcgttgccgg cgagatccca ggccatgagc ttggcccacg cgagcgcatc
     1741 gaccggctcg aa
//
LOCUS       DASWAV010000158         3220 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U2665
            k141_608054, whole genome shotgun sequence.
ACCESSION   DASWAV010000158 DASWAV010000000
VERSION     DASWAV010000158.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960316
            Sequence Read Archive: SRR10260007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 3220)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3220)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:47:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,929
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3220
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_608054"
                     /isolate="SMAG_U2665"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.36444 N 132.31889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..604
                     /locus_tag="VF958_03150"
     CDS             <1..604
                     /locus_tag="VF958_03150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="cytochrome C"
                     /protein_id="HEX9148135.1"
                     /translation="LAFDPEGTIFVTVQGGNLVGRLIPKTGKVDLREVPTPRARPYGI
                     VVGPDGAAWFCEFGSNKIGRLDPRTLEIREYPLPEGARPRRIASNLDGFLYYSDFARG
                     FIGRLDPKSGKVEEWASPGGRRSQPYGIASTRDGTVWYSESGVEPNTLVAFDPKAKSF
                     AKWPIPSGGGVVRNMVATPDGKLFLALSGVNKVAVAEATR"
     gene            complement(608..1012)
                     /locus_tag="VF958_03155"
     CDS             complement(608..1012)
                     /locus_tag="VF958_03155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005484653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HEX9148136.1"
                     /translation="MPARKKAPTRPRSDLRRVSRALRRYALSFPGATEDFPWGERVVK
                     VKGKVFVFLGMDPVPGGAIGLSVKLPESGPDALDLPFAKPTGYGLGKSGWVSATFQAK
                     DRLPLEILKGWIRESYCAVAPKKLVAELESPA"
     gene            1048..1518
                     /locus_tag="VF958_03160"
     CDS             1048..1518
                     /locus_tag="VF958_03160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014399014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEX9148137.1"
                     /translation="MEAVLFIGVQGCGKTTFYKERLFETHVRISRDMVKTKARERILI
                     GACLAAMQPFVLDNTNAVASQRAEIIGPAHAAGFRVVACYFRCGMREALWRNRRRPAG
                     AVVPAPGVIATFKKLQPPSWEEGFDEIRVISTDGADGFLVEKWSREKGDPSRPE"
     gene            1566..1988
                     /locus_tag="VF958_03165"
     CDS             1566..1988
                     /locus_tag="VF958_03165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9148138.1"
                     /translation="MRTTVRVTLALLVLALVGAPAFAGAAKDLEKTPQAKAYRLLLKA
                     VDDGDYEGYKKAMVAEAAKSIDEQTKEMGMTPKKGMDFLKAMAPTDVKLTDLKVDGKK
                     ATLSATGKQGGETNWGTIELAEENGVWKVGHQSWTNKH"
     gene            2192..2737
                     /gene="msrA"
                     /locus_tag="VF958_03170"
     CDS             2192..2737
                     /gene="msrA"
                     /locus_tag="VF958_03170"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009288335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HEX9148139.1"
                     /translation="MPQRGSAIFAGGCFWCEETAFEGVPGVISVTSGYTGGQKKDPTY
                     EEVSSGGTGHAESVQVVYDPSKISYEKLLDIFWHNVDPFQANGQFCDHGTQYRSAIFY
                     RDDAQRAAAEASKRKLEEDARFRGRIVTQIVPASTFYPAEEYHQDFYKKDPVRYHSYR
                     AGCGRDARLKAIWGDAAGGQK"
     gene            2782..>3220
                     /gene="msrB"
                     /locus_tag="VF958_03175"
     CDS             2782..>3220
                     /gene="msrB"
                     /locus_tag="VF958_03175"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020160930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033743 - peptide-methionine (R)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HEX9148140.1"
                     /translation="MAVGQSTAVRQKGWVASNFRKPSDEELRKRLTPLQYQVTQREGT
                     ERAYANEYWENHAAGIYVDVVSGEPLFSSLDKYESGTGWPSFTKPLEKANIKTGTDFK
                     IGLPRTEVRSVHADSHLGHVFDDGPPPTGLRYCMNSAAMRFIPV"
BASE COUNT          605 a         1030 c         1092 g          493 t
ORIGIN      
        1 cctcgccttc gacccggaag ggacgatctt cgtcaccgtc caggggggca acctcgtcgg
       61 aaggctgatc ccgaagaccg gcaaggtcga tctgcgcgaa gtgccgacgc cacgcgcgcg
      121 gccctacggg atcgtcgtcg gtccggacgg agccgcctgg ttctgcgaat tcggcagcaa
      181 caagatcggg cgcctcgatc cgcgaacgct cgagatccgg gagtatccgc tgccggaggg
      241 agcgcgtccg cggcgaatcg cgagcaacct ggacggattc ctctactaca gcgacttcgc
      301 gcgcgggttc atcgggcgtc tggacccgaa aagcgggaaa gtcgaggagt gggcgtctcc
      361 cggcgggcgg cgctcgcagc cttacggaat cgcttcgacg cgggacggca ctgtctggta
      421 cagcgaatcc ggcgtcgaac cgaacaccct cgtggctttc gatccgaagg cgaagtcgtt
      481 cgcgaagtgg ccgatcccct ccggcggcgg cgtcgtgcgc aacatggtgg cgacccccga
      541 cgggaagctc tttctcgcct tgagcggcgt gaacaaggta gcggtggcgg aggcgacccg
      601 ctgagtctca ggcgggagat tcgagctcgg cgacgagttt cttcggggcg acggcgcaat
      661 agctctcccg gatccagccc ttgaggatct cgagcgggag ccggtctttt gcctggaagg
      721 ttgcgctcac ccagcccgac tttcccaggc cgtagccggt cggctttgcg aaaggaaggt
      781 cgagagcgtc cgggccggac tcggggagct tgaccgagag gcctatcgcg ccgcccggca
      841 cgggatccat gcccaggaac acgaagacct ttcctttcac cttcacgaca cgctcgcccc
      901 agggaaagtc ctcggtcgca ccggggaagg agagcgcgta ccggcggagc gcccgcgaga
      961 cgcggcggag atcacttcgc ggacgggttg gggccttctt ccgggcgggc atggcgttcc
     1021 tgagcccgcg cagtatacgt tcggctcgtg gaagcggtcc tcttcatcgg cgtccagggc
     1081 tgcggcaaga cgacgttcta caaggagcgc ttgttcgaga cgcacgtccg gatcagccgg
     1141 gacatggtca agacgaaagc ccgagagcga atcctcatcg gcgcttgcct cgcggcgatg
     1201 caaccctttg tcctcgacaa cacgaacgcc gtcgcgagcc agcgcgcgga gatcatcggt
     1261 cccgcgcacg ctgccggttt ccgcgtcgtc gcgtgctact tccgctgcgg gatgcgcgag
     1321 gcgctctggc gaaaccgccg acgcccggcc ggcgccgtcg tccccgcccc cggcgtgatc
     1381 gcgaccttca agaagctcca gccgccgtcc tgggaggaag ggttcgacga aatccgggtg
     1441 atttccacgg acggggcgga cggcttcctc gtcgagaagt ggagccggga gaagggcgat
     1501 ccgtcgcgcc cggagtgaga gacaatagag gacacaccaa cttttcgacg aaggagcacc
     1561 gaaccatgcg caccaccgtc cgggtcactc tcgccctgct cgtcctcgca ctcgtcggcg
     1621 caccggcgtt cgccggcgcg gccaaagacc tcgagaagac gccgcaggcg aaggcgtacc
     1681 gactcctgct caaggccgtg gacgatggcg actacgaggg gtacaagaag gccatggtcg
     1741 ccgaggcggc gaagtcgatc gacgagcaga cgaaggaaat gggaatgacc ccgaagaagg
     1801 ggatggattt cctgaaggcg atggcgccga ccgacgtcaa gctgacggac ctgaaggtcg
     1861 acggcaagaa ggcgacgctc tcggcgaccg ggaagcaggg gggcgagacc aactggggaa
     1921 cgatcgagct cgccgaagag aacggcgtct ggaaggtcgg ccaccagagc tggacgaaca
     1981 agcattagag aatgtcgcgg ggacaggcct cacaggcctg cacccgcctc ctgattcctc
     2041 ggagcgccga aactcccggc ccgacctgaa tccaggacca ggcttcgagc gttcttcttg
     2101 cgaggaacga aggaggtctc tcttgagatt acgtattctt gccgccctgg cattcgggct
     2161 cgccgtcgcc gccctggcgg tcgcggctcc gatgccgcag cgagggtccg cgatcttcgc
     2221 gggcggctgc ttctggtgcg aggaaacggc gttcgaggga gtccccgggg tgatctccgt
     2281 gacgtccggg tacaccggtg gtcagaagaa agaccccacc tacgaggagg tttcctcggg
     2341 cgggaccggc cacgccgagt cggtacaggt cgtctacgac ccgtcgaaga tcagctacga
     2401 gaagctgctc gacatcttct ggcacaacgt cgatccattc caggcgaacg gtcagttctg
     2461 cgaccatggc acgcagtacc gttcggcgat cttctaccgg gacgacgcgc agcgcgcggc
     2521 cgccgaagcg tcgaagcgca agctcgagga ggacgcgcgc ttccggggaa ggatcgtgac
     2581 gcagatcgtt cccgcctcga cgttctaccc ggccgaggag tatcaccagg acttctacaa
     2641 gaaggacccg gtccggtatc actcgtaccg cgccgggtgc ggccgcgacg cgcgtttgaa
     2701 agccatctgg ggcgacgcgg caggaggtca gaagtgaaga tcacgagcgc cgccctgacg
     2761 gcgatcctcg cgctcacctc gattgcggtc gggcagagta cggccgtgcg gcagaaggga
     2821 tgggtcgcgt ccaacttccg aaagccgtcc gacgaagagc tgcgcaagag gctgactccg
     2881 ctccagtacc aggtgactca gagggaaggc accgagcgcg cgtacgcgaa cgagtactgg
     2941 gagaaccacg ccgccggcat ctacgtcgac gtcgtctccg gcgagccgct cttctcctcg
     3001 ctcgacaagt acgagtcggg aaccggctgg ccgagcttca cgaaaccgct cgagaaggcg
     3061 aacatcaaga cgggaacgga cttcaagatc ggcctgccgc ggaccgaggt tcgctcggtg
     3121 catgcggact cgcacctggg ccacgtgttc gacgacgggc cgccgccaac cggtctgcgc
     3181 tactgcatga actccgcggc gatgcgcttc atccccgtcg
//
LOCUS       DASWAV010000159         1577 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U2665
            k141_648703, whole genome shotgun sequence.
ACCESSION   DASWAV010000159 DASWAV010000000
VERSION     DASWAV010000159.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960316
            Sequence Read Archive: SRR10260007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 1577)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1577)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:47:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,929
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1577
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_648703"
                     /isolate="SMAG_U2665"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.36444 N 132.31889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1577
                     /locus_tag="VF958_03180"
     CDS             <1..>1577
                     /locus_tag="VF958_03180"
                     /inference="COORDINATES: protein motif:HMM:NF024907.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="VCBS repeat-containing protein"
                     /protein_id="HEX9148141.1"
                     /translation="GLAASAEKSREVRIPTDDDGVKSLVSRKGAQEKAARGLSAFHEF
                     RFSDRVVESGITFVEHPTDDSAKFYKSNHYDHGTGIAAADVDGDGLVDLYFVDQLGGN
                     ELWRNLGRGRFENVTGPAGVALPGRISVSASFADVDNDSDPDLFVTTVRGGNVLFEND
                     GKGRFRDVTRESGLSYSGHSSGAVFFDFDNDGRLDLFLVNVGRYTTEEKGRGGYFVGF
                     GDAFSGHLKPERTERSRLYRNLGNLRFADVTDEVGPFDSGWSGDASFADLDGDRFPEL
                     YVLNMQGDDHYFVNLQGKHFVDETARHFTKTPWGTMGIKFFDFDNDGRMDLLLTDMHS
                     DMSAEIGPDREKEKSRMEWTDAFLQGGANNVFGNAFYRNLGGGRFEEISDRVGVENYW
                     PWGPSVGDLNADGWDDVFIASGMGFPFRYGINSLLLNAGGRSFSDAEFLLGIEPRRGG
                     RTAAPAFDLDCATEGEGRKACVGQSGPITVQGTLSSRSSVIFDLDGDGDLDIVTNEFN
                     AAPQVLVSDLSARKRVH"
BASE COUNT          282 a          491 c          537 g          267 t
ORIGIN      
        1 cgggactcgc cgcgtccgcc gagaagagcc gtgaggtccg gattccgacc gacgacgacg
       61 gcgtgaagag cctcgtctcc cggaaaggtg cgcaggagaa ggcggcccgc ggtctttccg
      121 ccttccacga gttccggttc agcgaccgcg ttgtcgagag cgggatcacc ttcgtcgagc
      181 acccgaccga cgactcggcg aagttctaca agtcgaacca ctacgatcac ggaacgggga
      241 tcgccgccgc cgacgtcgac ggggacggcc tcgtggacct gtatttcgtg gaccagctcg
      301 gcgggaacga gctctggagg aacctcggcc gagggcgctt cgagaatgtg accggccccg
      361 cgggagtggc tctccccgga cggattagcg tctccgcgtc tttcgccgac gtcgacaacg
      421 actccgaccc cgaccttttc gtgacgaccg tccggggagg aaacgtcctc ttcgagaacg
      481 acggcaaggg gcgtttccgc gacgtcacga gggaatcggg cctctcctac tccgggcact
      541 cctcgggtgc ggtcttcttc gacttcgaca acgacggccg gctcgacctc ttcctcgtca
      601 acgtcggcag atacacgacc gaagagaaag ggcgtggcgg ctacttcgtt gggttcggcg
      661 acgcgttttc cgggcacctg aaaccggagc gaaccgagag gagtcggctc tatcggaatc
      721 tgggaaatct ccggttcgcc gacgtcaccg acgaagtcgg gccgttcgac tcgggctgga
      781 gcggtgacgc gagcttcgcg gacttggacg gcgaccggtt tcccgagctc tacgtcctga
      841 acatgcaggg ggacgaccac tacttcgtga acctgcaggg aaagcatttc gtcgacgaga
      901 ccgcccggca tttcacgaag accccgtggg ggacgatggg gatcaagttc ttcgacttcg
      961 acaacgacgg ccggatggac ctgctcctga ccgacatgca ctcggacatg agcgcggaga
     1021 tcgggcccga ccgggagaag gagaagtcgc ggatggagtg gaccgacgcg tttctgcagg
     1081 ggggcgcgaa caacgtcttc ggcaacgcct tctaccggaa cctgggcggc ggccgcttcg
     1141 aggaaatctc cgaccgcgtc ggcgtcgaga actactggcc gtggggtccc agcgtaggag
     1201 acctgaacgc cgacgggtgg gatgacgtct tcatcgcctc cgggatgggg ttcccgtttc
     1261 gctacggcat caattcgctc cttctcaacg ccggcggccg cagtttttcg gacgcggagt
     1321 ttctcctcgg gatcgagccg cggcgcgggg gccgaacggc agccccggcc ttcgatctcg
     1381 actgcgcgac cgagggggag gggcggaagg cgtgcgtcgg tcagagcggg ccgatcacgg
     1441 tgcagggaac tctgagctcg cggtcgtcgg tcattttcga cctcgacggc gatggagacc
     1501 tcgacatcgt gacgaacgag ttcaacgccg cgccgcaggt gctcgtgagc gatctctcgg
     1561 cgaggaagcg cgttcac
//
LOCUS       DASWAV010000160         2456 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U2665
            k141_409642, whole genome shotgun sequence.
ACCESSION   DASWAV010000160 DASWAV010000000
VERSION     DASWAV010000160.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960316
            Sequence Read Archive: SRR10260007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2456)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2456)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:47:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,929
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2456
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_409642"
                     /isolate="SMAG_U2665"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.36444 N 132.31889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..387
                     /locus_tag="VF958_03185"
     CDS             <1..387
                     /locus_tag="VF958_03185"
                     /inference="COORDINATES: protein motif:HMM:NF013936.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043169 - cation binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HEX9148142.1"
                     /translation="GAFVARAAGDTETASTWYEAFSSGLRRHIAVEEQILFPIFEERS
                     GLPPDSGPTEVMRAEHREILRLLGEILQTIGDPAKLPDEKRAAFHEILEEHHLKEEGM
                     LYPALDEVLTSDEGDALVLRIQSFAG"
     gene            447..923
                     /locus_tag="VF958_03190"
     CDS             447..923
                     /locus_tag="VF958_03190"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEX9148143.1"
                     /translation="MAVEAEMTFRRILFATDFSPASRAAFRVAVQLARRERGRLVIAH
                     VLPSVVSLGVETYVSARMYEEMETSARRWAQKRLDSLVEQARRSRVAARTVLLAGAAD
                     EGVSSAAAKERADLIVIGTHGRTGLERVFMGSVAARIIGTAPCPVLTIRPRRSSKR"
     gene            1038..1490
                     /locus_tag="VF958_03195"
     CDS             1038..1490
                     /locus_tag="VF958_03195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004838850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HEX9148144.1"
                     /translation="MLNVTRWEPFREFEKLPEQMARFFGFPAIRPYTTEETLTVADFM
                     PPVDIYETEKEYLIKLDLPEVTKEEVKVYVEDGVLTIQGERRKEKEEKGKKFHRVERS
                     YGTFLRTFTVPPDADETKIDAEFKEGLLKVHLPKSEKAKPKTIDVKVF"
     gene            1650..2141
                     /locus_tag="VF958_03200"
     CDS             1650..2141
                     /locus_tag="VF958_03200"
                     /inference="COORDINATES: protein motif:HMM:NF012780.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEX9148145.1"
                     /translation="MHKQPASATPKKPPTVRRREPTVRDLMTPNPYTLAPRDTLASLY
                     DLMDTRRVRHVPIVDKDGELVGLLTHTDLAMTALGSLADLPLSQERDLLQRRRIREVM
                     VTEVETVEPDSPLAEAAQTLFENKIGCLPVVEGSRLVGILTESDFVRRFVEEEAGREG
                     KDS"
     gene            2174..>2456
                     /locus_tag="VF958_03205"
     CDS             2174..>2456
                     /locus_tag="VF958_03205"
                     /inference="COORDINATES: protein motif:HMM:NF016184.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur transferase domain-containing protein"
                     /protein_id="HEX9148146.1"
                     /translation="MKRLVLFPGLGAAAMLLAVFGFGATCADIDKFYWIDERIATGGQ
                     PKLSDLASLKKEGFKTIINLREASEYDAAAEEAEARRLGLGYVSIPVRTA"
BASE COUNT          464 a          790 c          797 g          405 t
ORIGIN      
        1 ggcgcattcg ttgcgcgcgc cgcgggggac acggagaccg cctccacgtg gtacgaagcg
       61 ttctcttccg gcctgcgccg ccacatcgcc gtcgaagagc agatcctctt cccgatcttc
      121 gaggagcgct cgggtctccc ccccgattcc ggacccacgg aggtgatgcg ggcggagcat
      181 cgggagatcc tgcggttgct gggcgagatc ctccagacga tcggagatcc cgcgaagctg
      241 ccggacgaga agcgggcggc gttccacgag atcctggaag agcatcacct gaaagaggaa
      301 ggaatgctct atccggccct ggacgaggtg ctcacttccg acgagggtga cgccctcgtc
      361 ttgcggatcc agagcttcgc cggctgagcg ggcgcgcacc gcgctgattc gattggatca
      421 ggcgaggccg tcccctccat gccagcgtgg cggtggaggc cgagatgacg tttcgaagga
      481 ttctcttcgc cactgacttc tctcccgcgt cccgcgccgc ctttcgtgtc gccgtccagc
      541 tcgcgcgccg tgagcgcggg cgcctcgtga tcgcgcacgt gctcccttcc gtggtttcgc
      601 tcggcgtcga gacctacgtg agcgcccgga tgtacgagga gatggagaca tcggcacgcc
      661 gctgggcgca gaagcgcctg gactctctcg tcgagcaggc ccggcgttcg agggtcgccg
      721 cccggaccgt tctcctcgcg ggcgccgccg acgaaggggt ctcgagcgca gcggcgaagg
      781 aacgggccga cttgatcgtc atcggtactc acggccggac gggactcgag cgagtcttca
      841 tgggcagcgt cgcggcccgg atcatcggca ccgcaccctg cccggttctc acgattcggc
      901 cgcggagatc atcgaagaga tgacgcagcg cggttgaacc gattgcacca attcgaacgc
      961 accatgcgcg aaaagcgaaa cgggagaaca atcagcgtgg cccaaatcac tgggctctcg
     1021 agtaaggagg tgggccaatg ttgaatgtca cgcgttggga acctttccgg gagttcgaga
     1081 agctccccga gcagatggcg cggttttttg gcttcccggc catccgcccc tacacgaccg
     1141 aggaaaccct gacagtcgcg gacttcatgc cgcccgtgga catctacgag accgagaagg
     1201 aatatctgat caaactcgat ctccccgaag tcaccaagga agaggtgaag gtctacgtcg
     1261 aggacggagt gctgacgatc cagggcgagc ggcgaaagga gaaggaggag aaggggaaga
     1321 agttccatcg ggtcgaacgc tcctatggga ccttcctgcg gaccttcacc gttcctccgg
     1381 acgccgacga aaccaagatc gacgccgagt tcaaggaagg actgctgaag gttcacctcc
     1441 ccaagtcgga gaaggcgaag ccgaagacga tcgacgtcaa ggtcttctga ccttccggcg
     1501 ggcccccggc ggaagggctc gcgggcggat cggcgagagc aggtccgcct ttctttttgc
     1561 gccgcgagct gatgcattcg aatcatcccg gtacttctcg gcagaagaca gaatcgtcgc
     1621 gtcgggcttc gcagcgaaag gaaggaacca tgcataaaca gcccgcctcc gcgaccccga
     1681 agaaaccgcc aaccgtccgg cggcgggagc cgactgtgcg cgatctcatg acgccgaacc
     1741 cctacacgct cgctccccgt gacacgctcg cgtcgcttta cgacctcatg gacacccgtc
     1801 gcgtccgaca cgtgccgatc gtggacaagg acggcgagct cgtggggctc ctcacccaca
     1861 cggacctcgc gatgacggcg ctcggctccc tcgccgatct gcctctcagc caggagcgtg
     1921 atcttctcca gcgccggagg attcgggaag tgatggtgac cgaggtcgaa acggtggaac
     1981 cggacagccc gctcgccgaa gcggcgcaga ctctgttcga gaacaagatc gggtgtcttc
     2041 ccgtcgtcga gggaagtcgc ctcgtcggga tcctgaccga gtcggacttc gtgcgccgct
     2101 tcgtcgagga agaggccggg cgcgaaggaa aggactcctg aagatcggtc cggatcgaaa
     2161 ctgtgaggtc cacatgaaac gcctggttct tttccccggt ctcggcgccg ccgcgatgct
     2221 cctcgccgtc ttcggcttcg gcgcgacgtg cgccgacatc gacaagttct actggatcga
     2281 cgagcggatc gcgacgggcg gccagccgaa gctctccgac ctcgcttccc tcaagaaaga
     2341 gggcttcaag acgatcatca acctgcggga ggcctcggaa tacgacgccg ccgccgaaga
     2401 ggcggaggcg cgccggctgg ggctcggcta cgtctcgatc ccggtccgga ccgcct
//
LOCUS       DASWAV010000161         3228 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U2665
            k141_288407, whole genome shotgun sequence.
ACCESSION   DASWAV010000161 DASWAV010000000
VERSION     DASWAV010000161.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960316
            Sequence Read Archive: SRR10260007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 3228)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3228)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:47:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,929
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3228
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_288407"
                     /isolate="SMAG_U2665"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.36444 N 132.31889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1243)
                     /locus_tag="VF958_03210"
     CDS             complement(<1..1243)
                     /locus_tag="VF958_03210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013566956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HEX9148147.1"
                     /translation="MTEVASPERRNFSKIRSFMDLPNLIDVQRRSYERFLQMNLLPEE
                     REDSGLQAVFTSVFPFSDFRGACELQFVKYSIGNWECKCGRLKGLEHLRMDCRSCGAR
                     IVTAHPHEETVTCPRCGTQNPNRVTLCDVCGNPVDLQMKYSVAECQERGMTYSVPLKV
                     TFRLFVYDKDPETGVKTMRDAKEEEVFFGDIPLMTEHGTFVINGTERVIVSQLHRSPG
                     VFFTRESKQGFLAKIIPYRGSWVEFEFDQKGVLFVRIDRKRKFPATIFLRALGLETDE
                     SILRQFYATVPARFERGRAYLTVSKEVLKQEELKERYARVKRETKTAFAGIRLSGEDQ
                     REIAKKGSIERSVDDAAVEKACFLADVVDLSTGEVLFEAGQELGADAVEGLKERGIKE
                     IELFFPDWDLSGDVLVNTIRKD"
     gene            complement(1376..1762)
                     /gene="rplL"
                     /locus_tag="VF958_03215"
     CDS             complement(1376..1762)
                     /gene="rplL"
                     /locus_tag="VF958_03215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012513304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HEX9148148.1"
                     /translation="MAMTADNFVKEIENMTVLELNSLVKALEEKFGVSAAAMAMPMAA
                     AGAPAGAAAAPAEEEQTEFAVTLVAAGEKIKTIKVVREVTSLGLKEAKDLVDGAPGLL
                     KEGVSKEEAANIKKKFEEVGAKVEIK"
     gene            complement(1795..2340)
                     /gene="rplJ"
                     /locus_tag="VF958_03220"
     CDS             complement(1795..2340)
                     /gene="rplJ"
                     /locus_tag="VF958_03220"
                     /inference="COORDINATES: protein motif:HMM:NF000955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HEX9148149.1"
                     /translation="MERGEKTAAVAELQSELAKSRNAILFGFAGLKVPEITELRRQVR
                     GTRSRYLVVKNTLALRATRGTPFEEVSRHFAGMTAVAFNQNDPVGLAKVLTTFAKTNP
                     NLVFKGALIEGKAVEASEIKTIAELPTREELVARLVSLMQSPLTRLVNVLAGPMSNLA
                     RVLRAVAEKSNSGAADTAPTS"
     gene            complement(2344..3039)
                     /gene="rplA"
                     /locus_tag="VF958_03225"
     CDS             complement(2344..3039)
                     /gene="rplA"
                     /locus_tag="VF958_03225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005038373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HEX9148150.1"
                     /translation="MAKTGKKFQAAKKKVEPRPYGLADAVKLLREVRFAKFDESVDVA
                     MLLGVNPKHADQMVRGTVVLPHGTGKSKRVAVITSGDKLKEAQDAGADHVGGEDLVAR
                     IQEGFLDFDALVATPDMMRHVGRLGKTLGPRGLMPNPKAGTVTMEVGKAVREIKGGKI
                     EFRVDKTAIIHAPIGKVSFDEKKLEENAAAFIHAVQKAKPPAAKGKYFRTVSVSSTMG
                     PGIEIDTASIEGL"
     gene            complement(3102..>3228)
                     /locus_tag="VF958_03230"
     CDS             complement(3102..>3228)
                     /locus_tag="VF958_03230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004233911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HEX9148151.1"
                     /translation="VTKAQVEEIAKTKLPDLNAADLPSAIRVIEGTARSMGIEVV"
BASE COUNT          514 a         1114 c          961 g          639 t
ORIGIN      
        1 tgtccttgcg aatcgtgttg acgaggacgt ctccggagag gtcccagtcg gggaaaaaga
       61 gctcgatctc cttgatgccg cgctccttca acccctcgac cgcgtccgca ccgagctcct
      121 gcccggcctc gaagaggact tcacccgtcg acaagtcgac gacgtcggcg aggaagcacg
      181 ccttctcgac cgccgcgtcg tcgacggagc gctcgatcga tcccttcttc gcgatctcgc
      241 gctggtcctc tccagagaga cggatgcccg caaacgccgt cttcgtctcg cgcttgacgc
      301 gcgcgtagcg ttccttcagc tcctcctgct tcagcacttc cttgctgacc gtcaggtagg
      361 cccggcctcg ctcgaaacgc gccggaacgg tcgcgtagaa ctgccggagg atcgactcgt
      421 ccgtttcgag accgagcgca cgcaggaaga tcgtcgccgg gaacttccgc ttccgatcga
      481 tccgcacgaa gagcacgccc ttctggtcga actcgaactc cacccaggag ccccggtacg
      541 ggatgatctt ggcgaggaag ccctgtttcg actcgcgggt gaaaaagacg cccggcgagc
      601 ggtggagctg cgaaacgatg acccgctccg tgccgttgat gacgaacgtc ccgtgctcgg
      661 tcatcagcgg gatgtcgccg aagaagacct cctcttcctt cgcgtcgcgc atggtcttga
      721 cgccggtctc cgggtccttg tcgtagacga agagacggaa ggtgaccttg agcgggacgg
      781 agtacgtcat cccccgctcc tggcactccg cgaccgagta cttcatctgc aggtcgacag
      841 ggttgccgca gacgtcgcag agcgtgaccc ggttggggtt ctgcgtgccg cagcgcgggc
      901 acgtgaccgt ctcctcgtgc gggtgcgcgg tcacgatccg cgcgccgcag gaccggcagt
      961 ccatgcgcag gtgctccagg cccttcaggc ggccgcactt gcactcccag ttgccgatcg
     1021 agtacttgac gaactgaagc tcgcaagcgc cccggaagtc ggagaacgga aagacgctcg
     1081 tgaagacggc ctgcagcccg gagtcctcgc gctcctccgg cagaaggttc atctgcagga
     1141 agcgctcgta ggaccggcgc tggacgtcga tcagattcgg aagatccatg aacgaccgga
     1201 tcttcgagaa gttccggcgc tcgggtgatg cgacttcagt catttatgag ggctccctgt
     1261 agcgcgccgg acacgccggc gcgatttagc acgaggcacg aagaaagccg gccacagggc
     1321 gcggattgcc ccgcacccat cgaaacagtc gcgtattccc tggtcagcgc ggtccctact
     1381 tgatctcgac tttcgcgccg acttcctcga acttcttctt gatgttggcg gcctcttcct
     1441 tcgagacccc ctccttcaat agccccggcg cgccgtccac gaggtccttg gcttccttca
     1501 agccgagact cgtgacttcc cggacgacct tgatcgtctt gatcttctcg cccgccgcga
     1561 cgagggtgac cgcgaactcg gtctgctcct cctcggcggg agccgccgcg gcgccggccg
     1621 gcgcccctgc ggccgccatc ggcatcgcca tcgccgccgc cgagacaccg aacttctctt
     1681 cgagcgcctt gacgagcgaa ttgagctcga gaaccgtcat gttctcgatc tctttgacga
     1741 agttatctgc ggtcattgcc atctccgtat cctctcctct tgagtttcga cgactcaact
     1801 cgtgggagcg gtgtccgccg cccccgaatt cgacttctcc gcaaccgcgc gcagcaccct
     1861 cgccaggttc gacatcggac cagcaagcac gttgacaagc ctcgtcagcg gcgactgcat
     1921 gagggagacg agccgcgcca ccagctcttc acgcgtcggc agctccgcga tcgtcttgat
     1981 ctcggaagcc tcgacggcct tcccctcgat gagagcgcct ttgaagacca ggttcgggtt
     2041 ggtcttggcg aaggtcgtca ggaccttcgc aagcccgacc gggtcgttct ggttgaacgc
     2101 caccgccgtc atgccggcga agtggcgcga gacctcttcg aacggcgttc cgcgggtcgc
     2161 ccgcagcgcg agcgtgttct tgaccaccag gtatcgcgag cgggtgccgc gcacctgccg
     2221 acgaagctcc gtgatctccg ggactttcaa cccggcgaac ccgaagagga ttgcgttgcg
     2281 cgatttggcg agctccgact gaagctcggc gaccgcggcc gttttttctc ctctttccat
     2341 cgcctacaac ccctcgatcg acgcggtgtc gatctcgatg cccggcccca tcgtcgagga
     2401 gaccgagact gtccggaagt acttgccctt ggcggcgggc ggtttcgcct tctggacggc
     2461 gtggatgaaa gcggccgcgt tctcctcgag cttcttctcg tcgaaggaga ccttgccgat
     2521 cggcgcgtgg atgatcgccg ttttgtccac gcgaaattcg atcttgccgc ccttgatctc
     2581 ccggaccgcc tttccgacct ccatcgtcac ggtgcccgcc ttcggattgg gcatgaggcc
     2641 gcggggcccg agcgtctttc cgagccgccc aacgtggcgc atcatgtcgg gggttgcgac
     2701 gagcgcgtcg aagtccagga agccctcctg gatcctcgcg accaggtcct cgcctcccac
     2761 gtggtcggcg ccggcgtcct gggcctcctt caacttgtcg cccgaggtga tcaccgccac
     2821 ccgcttgctc tttcccgttc cgtgcggcag gacgaccgtc ccgcgcacca tctggtcggc
     2881 gtgcttgggg ttgacgccga ggagcatcgc gacgtcgacg gactcgtcga acttcgcgaa
     2941 ccggacctcg cgaaggagct tgaccgcgtc cgcgagcccg tacggccgcg gctcgacctt
     3001 cttcttcgct gcctgaaact tcttgccggt ttttgccatt ggattccttt cgtcgtgcaa
     3061 acggcgcgtt ttcgcgcctc cgactcaaaa aggcgacgcc tctagacgac ctcgattccc
     3121 atgctccgcg ccgttccctc gatcacccgg atcgccgacg gcaggtccgc tgcattcagg
     3181 tcagggagct tcgtcttcgc gatctcctcg acctgggctt tcgtcacc
//
LOCUS       DASWAV010000162         3033 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U2665
            k141_249740, whole genome shotgun sequence.
ACCESSION   DASWAV010000162 DASWAV010000000
VERSION     DASWAV010000162.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960316
            Sequence Read Archive: SRR10260007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 3033)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3033)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:47:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,929
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3033
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_249740"
                     /isolate="SMAG_U2665"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.36444 N 132.31889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(153..1280)
                     /locus_tag="VF958_03235"
     CDS             complement(153..1280)
                     /locus_tag="VF958_03235"
                     /inference="COORDINATES: protein motif:HMM:NF025433.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LssY C-terminal domain-containing protein"
                     /protein_id="HEX9148152.1"
                     /translation="MTIARTRGKAGPGRLARGLRAAALVGLLLPAYATLAYVLIPAVW
                     RRHAKLPAEVALPRLSYTAEGIPADPINVAVIGTAGEILAAMRAAGWTQADRITFKSG
                     LRDAGSVLFERPYTAAPMSTHFLARHRPQDLAFEQLVGRSPRRRHHVRFWRQGPPDAH
                     GRALWVGAASFDTSVGVSRFTGEVMHHIDPAVDRERDKLVNDLLRARRAPAVSWIPDF
                     AAARRGRNGGGDSYETDGRLAVLQLLGDEPEDLQRRLADPEVAAISPEREEVPIASVE
                     DVSRLAGCMDEEFRFGIRKVDDLAIARTIGHPEGTVQTIGADVDRRGVASLPRLQTED
                     RAQSGHRLENSVRKARAVEVFPAPPSRVRPEAPAQKRHGLP"
     gene            complement(1289..1576)
                     /locus_tag="VF958_03240"
     CDS             complement(1289..1576)
                     /locus_tag="VF958_03240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004511384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9148153.1"
                     /translation="MNVRRTLGALALSAAFASTALAAVRTYQVTGPVLEVRDDMVAVQ
                     KGKDRWEIARDSATSMKGASPKVGDKVTIEYRMTATSVDVKAPAASKKKKK"
     gene            complement(1660..2679)
                     /locus_tag="VF958_03245"
     CDS             complement(1660..2679)
                     /locus_tag="VF958_03245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histone deacetylase"
                     /protein_id="HEX9148154.1"
                     /translation="MTTGLVYDEAFLLHRAPYEHPEYPGRLAAIWKRLNEEGLAGRCR
                     RVAAREATREELRAVHTLEHIDRIAATADLSFAQLDPDTYTSRHSAAAARLAAGGLID
                     LVGKVLSGDVSNGLALLRPPGHHAEADRAMGFCLFNNVAVATKAAQRAGVERIAIVDW
                     DLHHGNGTQHTFENDPNVLYFSTHEYPFYPGTGAVREVGRGEARGRTVNVAWPPGMGD
                     VEYLAAFDRVLLPIARAFDPQLVLVSCGFDAGAGDPLGGMRVSAAGYAALTERVCSLA
                     EGRAVLALEGGYEPDAISAAAAACTAVLLGDAPPPLETGHPNAAAEHVLREVVEAQRP
                     YWPGL"
     gene            complement(2676..>3033)
                     /locus_tag="VF958_03250"
     CDS             complement(2676..>3033)
                     /locus_tag="VF958_03250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9148155.1"
                     /translation="PPEASTPRRRGPGIAVIGVTIYALLFLGALWYFKREPTVPRPKA
                     GVVAKPPTRLSLLAGEGLAPSARGKYLARIGADHCDCGCELSLAKCLARDRSCVRSPA
                     LATERLRAAASPARAP"
BASE COUNT          451 a         1011 c         1101 g          470 t
ORIGIN      
        1 tcgcccggat gcccgaggac gagctcgggt cgacgccgcc cggcgcggtc cggtcgcgtt
       61 tcctgatcga tgcgctgtcc tcggagtcga agtggtgcga cgacgtgcgg acgcccgcgc
      121 gagagagctg cggagagttt cggacgaaga cgctaaggga ggccgtgtct cttctgcgca
      181 ggcgcctcgg gacggacccg ggagggtggc gctgggaaga cctccaccgc gcgcgctttc
      241 cgcacggagt tttcgaggcg gtgcccggac tgcgctcgat cttcggtctg gagacggggc
      301 agggaggcga cgcctcgacg gtcaacgtcg gcgcctatcg tctggacggt tccttccgga
      361 tgaccgatgg tccgagctat cgccagatcg tcgacttttc ggatcccgaa tcgaaactct
      421 tcgtccatac aaccggccag tcgggaaacg tcttcgaccg acgctatcgg gacctcctcc
      481 cgctctggcg aaatggcggc tacttcggga tcggcgagcc tccgctgaag gtcctccggc
      541 tcgtcgccga gtagctgcag gacggcgaga cggccgtcgg tttcgtagga gtcgccgccc
      601 ccgtttcgtc cgcggcgcgc cgccgcgaaa tccggaatcc acgagaccgc gggcgcgcgt
      661 cgggcgcgca ggagatcgtt cacgagcttg tcgcgctcgc gatccaccgc cgggtcgatg
      721 tggtgcatga cttcgccggt gaacctcgag acaccgacgc tcgtgtcgaa gctggcggca
      781 cccacccaga gcgcgcggcc atgagcgtcg ggaggcccct gccgccagaa gcgaacgtgg
      841 tggcggcggc gggggctcct gccgacgagc tgctcgaacg cgaggtcctg aggccggtgc
      901 cgcgcgagga agtgagtgct catcggcgcc gccgtgtacg gccgttcgaa gaggacgctg
      961 cccgcgtcgc gcaaaccgct cttgaaagtg atgcgatccg cctgggtcca ccccgcggcg
     1021 cgcatcgcag cgaggatttc gccagctgta ccgatgaccg cgacgttgat cggatcggcg
     1081 gggatcccct cagccgtata gctcaaccgc gggagcgcga cctcggccgg gagcttcgcg
     1141 tgccgccgcc acacggccgg aatcagcacg tacgcgagcg tcgcgtacgc gggcagcagg
     1201 agtccgacga gcgcggccgc gcggagtccg cgggcgagcc gcccgggtcc ggcctttccc
     1261 ctcgtgcgcg cgatcgtcac ccgattccct acttcttctt cttcttcgag gctgccggtg
     1321 ccttgacgtc gaccgacgtc gccgtcatcc gatactcgat ggtgaccttg tcgccgacct
     1381 tcggcgaggc gcccttcatc gacgtggcgg agtcccgggc gatctcccag cggtccttcc
     1441 ctttttgcac ggcgaccatg tcgtcccgga cctccagcac ggggccggtc acctggtagg
     1501 ttcggaccgc ggccaaggcc gtggatgcga aggcggccga gagggcgagg gcgccgagtg
     1561 ttcgacgaac gttcatggag tctcctttct cgggaagggt agccgggggt gccgtgaacc
     1621 gggaatgaac gcgtgtggca agtggagacc tgatcctcct cagagtcccg gccagtaggg
     1681 gcgttgtgct tccacgacct cgcgcagaac gtgctccgcc gccgcgttgg ggtgtccggt
     1741 ctcgaggggt ggcggcgcgt cgccgaggag cacggccgtg caggcggcgg ccgcggccga
     1801 gatcgcgtcg ggctcgtaac cgccttcgag cgcgaggacc gcgcgtcctt cggcgagtga
     1861 gcacacgcgt tcggtcagcg ccgcgtatcc ggcggccgag accctcatgc cgccgagcgg
     1921 atcgcccgcg ccggcgtcga agccgcagga cacgaggacg agctgcggat cgaacgcgcg
     1981 ggcgatcggc agcagcacgc gatcgaaggc agcgagatac tccacatccc ccattcccgg
     2041 gggccaggcg acgttgacgg tgcgccccct cgcctcgccg cggccgacct cacggaccgc
     2101 gcccgtgccg gggtagaagg ggtattcgtg cgtcgagaag tagagaacgt tcgggtcgtt
     2161 ctcgaaggtg tgctgcgtgc cgtttccgtg gtgcaggtcc cagtcgacga tggcgattcg
     2221 ctcgacgccg gctctctgcg cggccttcgt cgcgaccgcc acgttgttga agaggcagaa
     2281 gcccatcgcg cgatcggcct ccgcgtggtg accgggcggc cgcaggagcg ccaacccgtt
     2341 cgacacgtcg cccgacagga ccttcccgac gagatcgatc aggccgccgg ccgcgagccg
     2401 cgctgcggcc gcggaatggc gcgacgtgta cgtgtcggga tcgagctgcg cgaagctgag
     2461 atccgcggtc gcggcgatcc gatcaatgtg ctcgagtgtg tgcacggccc gaagctcttc
     2521 gcgggtcgct tcgcgggcgg ccacccggcg acaacgaccg gcgagcccct cctcgttgag
     2581 tcgcttccag atcgcggcaa gacggccggg atactccggg tgctcgtagg gagcacggtg
     2641 gaggaggaat gcttcgtcgt agacgaggcc cgtcgtcacg gcgcgcgcgc cggagaggcg
     2701 gcggctcgca gacgctcggt ggcgagggcg ggactgcgga cgcacgagcg gtcgcgggcc
     2761 aggcacttcg cgagcgagag ctcgcatccg cagtcgcagt ggtcggctcc gattcgggcg
     2821 aggtacttgc cgcgcgcgga gggtgcgaga ccttccccgg cgagaagcga caggcgagtg
     2881 ggcggcttgg cgaccacccc ggccttcggc ctcggaaccg tgggctctct cttgaagtac
     2941 cagagagcgc ccaggaaaag gagcgcatag atcgtgaccc cgatcaccgc gatcccgggc
     3001 ccgcgccgtc ggggggtgga cgcctccggg gga
//
LOCUS       DASWAV010000163         3184 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U2665
            k141_47456, whole genome shotgun sequence.
ACCESSION   DASWAV010000163 DASWAV010000000
VERSION     DASWAV010000163.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960316
            Sequence Read Archive: SRR10260007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 3184)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3184)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:47:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,929
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3184
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_47456"
                     /isolate="SMAG_U2665"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.36444 N 132.31889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..942)
                     /locus_tag="VF958_03255"
     CDS             complement(<1..942)
                     /locus_tag="VF958_03255"
                     /inference="COORDINATES: protein motif:HMM:TIGR01891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004180 - carboxypeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HEX9148156.1"
                     /translation="MSSGSDRLDELLTDFPDREFQSIVETRRDLHRRPELAFQETRTA
                     DVIARELSSLGVEPRTGVAGTGVIADLQDGESRILLRADMDALPIIEASGVAFESETR
                     GKMHACGHDGHVAIALATARRLSRNPGGAVRFLFQPAEETGGGARACAAAGVLQGVRA
                     AIGLHLWNQLPVGRIGINRRALMAAVDEFEIHVTGRGGHGAAPHETADPIVAAARIVE
                     ALQTIVSREVSPLDSAVVTIGTIHGGDAFNVIPSSVRLTGTARWFAETTGAAMPGRIE
                     RIATHVAAAAGVTARLEYKRVNRPVVNDPRITEIVIQA"
     gene            1012..1650
                     /locus_tag="VF958_03260"
     CDS             1012..1650
                     /locus_tag="VF958_03260"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013742.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-methyltransferase"
                     /protein_id="HEX9148157.1"
                     /translation="MIPLVSSELDRYLIERLPDRHPVIAEMEAFATERDVPIVGPAVA
                     TLLAILARSIGARRVCELGSAIGYSTAFFAQAVGPGGSVLYTDGDERNARRAEGYLDR
                     LNLRDRVEIRVGEAVGILEASSGDFDVVFIDIDKDAYPHALEAAAPRVRPGGYLLADN
                     ALWSGKVVDPAIRDRATEGIREFNRRLFARRDFESVIVPLRDGVAIARRSER"
     gene            1650..2798
                     /locus_tag="VF958_03265"
     CDS             1650..2798
                     /locus_tag="VF958_03265"
                     /inference="COORDINATES: protein motif:HMM:NF024762.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX9148158.1"
                     /translation="MDRDELFLRFQFLASTEEIDVFEGALLIAVLIDPTEDLAAARAT
                     AAGLGAAVRDRIDWGEPGIDALRAVLFTDEGFQGDQETYDEPENSSVASVLARRRGMP
                     ITLSIVTVEVARRAGIRLAGIGLPGHFVVGGADLPEDTYLDPFDGGVLRDRESVARRV
                     SAVFGAPLALPEEIFAPDTERSVLARVLSNLRRSLERREKYADALETIRWSEVLDPEE
                     ITYRRERGLLLLKSGRTDEALASLETYVAGATGEDAEAVQKLVQVVRERGLTSAGPGL
                     VALSSPQKKIFTLEEARSLLPKVKELTSAAVFKFARLAETEESDDEQQGVVGEWAREI
                     LALGAEIKGLWLVDFDSGAGYYCWKYPEPSLEYFHGYEEGFAGRLPLQ"
     gene            3017..>3184
                     /locus_tag="VF958_03270"
     CDS             3017..>3184
                     /locus_tag="VF958_03270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009439054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium chelatase domain-containing protein"
                     /protein_id="HEX9148159.1"
                     /translation="MLARVHSAATFGIDARVLDVEVDVSAGLPHFTIVGLPDPSVREA
                     RERVKSALRNSG"
BASE COUNT          472 a         1084 c         1110 g          518 t
ORIGIN      
        1 ggcctggatg acgatctcgg tgatccgggg atcgttgacg acgggccggt tgacgcgctt
       61 gtattcgagc cgcgcggtga cgcccgctgc cgccgcgacg tgggtggcga tcctctcgat
      121 cctgccgggc atcgccgccc ccgtcgtttc ggcgaaccag cgagcggtcc cggtcaaccg
      181 cacgctcgac ggaatgacgt tgaacgcgtc cccgccgtga atcgtcccga tcgtgacgac
      241 cgcggagtcg agcggcgaca cctcccgcga gacgatcgtc tggagtgctt cgacgatcct
      301 cgccgccgcg acgatcggat cggcggtctc gtgcggcgcc gcgccgtggc ctccgcggcc
      361 ggtcacgtga atctcgaact cgtcgacggc ggccatcagc gcgcgccggt tgattccgat
      421 cctgccgacc gggagctggt tccagaggtg cagcccgatc gccgcccgga cgccctgcag
      481 aacgccggcc gccgcgcagg cgcgcgcgcc gcctccggtc tcctcggcgg gttgaaagag
      541 gaagcgcacg gcgccgcccg ggttccgcga gagccggcgg gcggtggcga gcgcgatcgc
      601 gacgtgccca tcgtggccgc aggcgtgcat ctttccccgt gtctccgatt cgaaggcgac
      661 gccactcgcc tcgatgatcg gcagcgcgtc catgtccgct cgcaggagga tccggctttc
      721 gccgtcctgg agatctgcga tgacgcccgt cccggcgacg ccggtacgcg gctcaactcc
      781 gagggacgag agctcccgcg cgatgacatc cgccgtcctc gtctcctgga aagcgagctc
      841 ggggcggcgg tggagatcgc gacgcgtctc gacgatcgac tggaactctc ggtccgggaa
      901 gtcggtcagc agttcgtcaa gccgatccga gccggaagac acgccgggat cttatagaac
      961 cgccggccgc acgcgacacc gaaggcgacc gccgaaagct atgattcctc tgtgattccc
     1021 ctcgtttctt ccgaactcga ccgctacctg atcgagcggt tgccggaccg ccaccccgtc
     1081 atcgccgaaa tggaggcgtt cgcgaccgag cgcgacgtgc cgatcgtcgg tccggcggtc
     1141 gcgaccctgc tcgcgatcct ggcccgctcg atcggggcgc ggcgcgtctg tgagctcgga
     1201 agcgcgatcg gatactcgac cgcgttcttc gcgcaggcgg tcgggcccgg cgggagcgtt
     1261 ttgtacaccg acggcgacga gaggaacgcg cgccgggccg agggctatct cgatcgcttg
     1321 aatctgcgcg atcgggtcga aatccgcgtc ggcgaggccg tcggaatcct cgaggcgagc
     1381 tccggggact tcgacgtcgt cttcatcgac atcgacaagg acgcctatcc gcacgcgctc
     1441 gaggccgcgg cgccgcgagt tcggcccggc gggtatctcc tcgccgacaa tgccctctgg
     1501 tcgggcaagg tcgtcgaccc ggcaatccgg gaccgcgcga cggaggggat ccgtgagttc
     1561 aaccgccgtc ttttcgcgcg gcgcgacttc gaatccgtga tcgttcctct ccgcgacggc
     1621 gtggcgatcg cgcggcggtc cgagcgctga tggaccgcga cgagctgttt ctccgttttc
     1681 agttcctcgc gtcgaccgag gagatcgacg ttttcgaggg cgcgttgctg atcgccgtgc
     1741 tgatcgaccc gacggaggac ctcgcagcgg cccgcgccac ggcggcgggg ctgggcgccg
     1801 ccgttcgcga ccggatcgac tggggagagc ccggaatcga cgccttgcgg gcggtgctgt
     1861 tcaccgacga aggattccag ggggaccagg agacgtacga cgagccggaa aactcgtcgg
     1921 tcgcgagcgt cctcgcgcgg cggcggggga tgcccatcac tttgtcgatc gtgaccgtgg
     1981 aggttgcgcg ccgcgccggg atccgcctcg ccggaatcgg gctgccgggc cacttcgtcg
     2041 tggggggcgc cgatctcccc gaagacacat atctcgaccc cttcgatggg ggcgtgctgc
     2101 gagaccgaga gtccgtcgcc cgccgggtga gcgcggtctt cggcgccccg ctcgctctcc
     2161 ccgaagaaat ctttgcgccc gacaccgagc gctcggttct cgcccgcgta ctgtcgaacc
     2221 tgcgacgctc gttggaacgg cgcgagaagt acgcggacgc gctcgagacg atccgctggt
     2281 ccgaggtgct cgatcccgaa gaaatcacgt accggcggga gcgggggctg ctcctgttga
     2341 agagcgggcg gaccgacgag gcgctcgcct cgctcgagac gtacgtggcc ggcgcgacgg
     2401 gcgaggacgc cgaagcggtt cagaagctgg tccaggtcgt gcgggaacgc gggttgacgt
     2461 ccgcgggacc gggtctcgtc gcgctgtcct ctcctcagaa gaagatcttc acgctcgagg
     2521 aggctcgttc gctgcttccg aaagtgaaag agctcacgtc ggccgccgtc ttcaagttcg
     2581 cgaggctcgc agaaaccgaa gagtccgacg acgagcagca gggcgtggtc ggcgagtggg
     2641 cgcgggaaat cctcgcgctc ggcgccgaga tcaagggact ctggctcgtg gacttcgact
     2701 cgggcgccgg ctactactgc tggaagtacc ccgagccttc actggagtac ttccacggct
     2761 atgaggaggg attcgcgggg aggctgccgc tgcaataagc cgtcgcgctc gcttgcgaag
     2821 gacctgctga cgcaccggca cgcggctcgg cggaccggcc tcgaccgcgt gcctggtcct
     2881 tcgctgcact cgcgctcccg cggcgcgagg ctcctccttc gattacctca ggagactcct
     2941 cgcgccgcga gctcagaatc ggtggactgc ggcgcgtgca ggtccgcgcg gcctgtcccc
     3001 gcaacaacta aagggcatgc tggcccgcgt ccactccgcc gcaactttcg ggatcgacgc
     3061 ccgcgttctc gacgtcgagg tggacgtgtc ggcggggctt ccccacttca cgatcgtcgg
     3121 cctgcccgac ccgagcgtcc gcgaagctcg tgagcgggtg aagtcggcgc tgcgcaacag
     3181 cggt
//
LOCUS       DASWAV010000164         3827 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U2665
            k141_288863, whole genome shotgun sequence.
ACCESSION   DASWAV010000164 DASWAV010000000
VERSION     DASWAV010000164.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960316
            Sequence Read Archive: SRR10260007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 3827)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3827)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:47:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,929
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3827
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_288863"
                     /isolate="SMAG_U2665"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.36444 N 132.31889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..897)
                     /locus_tag="VF958_03275"
     CDS             complement(<1..897)
                     /locus_tag="VF958_03275"
                     /inference="COORDINATES: protein motif:HMM:NF015251.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HEX9148160.1"
                     /translation="MSPIEAPGTGGGAPGTPRARRKWVSHGLSNRIVYGGLHYGARWL
                     PLTILKTISTVGNSIAVTLLRSTRRGMAENFERARIASDGDASRLARRVFFEYGRATI
                     DVWRLRSEAFTPVITTFEADARVLAASRREGRGFLLVTGHIGNWEMGAVTLRRHGLVP
                     AVVGQPELDPNVQEMRLELRERLGVESIDIGSSMATAFRVRSAVDRGRAVALLVDRAY
                     PEDQVIVPFFGRPTPFLRSPALLARFCGCDVLPGFFLRNADGSYRNVWGEPLQANPSL
                     SPEEDALRMMSRVAADLEEVVRA"
     gene            930..2375
                     /locus_tag="VF958_03280"
     CDS             930..2375
                     /locus_tag="VF958_03280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012470255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HEX9148161.1"
                     /translation="MNVLLLRPDPGNERFGLGPFFRVEPLGLEYVGAALRGRGHETTV
                     VDLRFGHRVSSSVQRTRPRIVGIACMHALELDEVLATAREVRRVAPDAFIVVGGHAAA
                     SYPAPLERPEIDAICVDDGEEVVPALADALARGRPASEVPALRLSTPDGWVSTPALAE
                     RTALDRIALPDRSLVDRDRNRYHCLLFKPVWLVETARGCPFRCSFCSVWQLYDRSFRE
                     RSIGAVVDDLASVGENVFIADDLFWNHPERSLALAEALKKRGVKKRWLLVQTRTDLVC
                     RRPDLLEAWRPIAKDFDIFFGLEAASDEGLAGVVKDTGVWASIEAARISRSFGYGVTG
                     NFLVDPDWNEDRFRELWAFVARYGFERAGYTILTPLPGTELYERLRPVIAGQPWFKYD
                     MHHVLWEPRLGARRFFELYAETWRRSILNTAGEKSLFDWMRQVRPSQIPYITRVLLRT
                     QRMMRPEAYLREHEAAERVAREIPAEPARAT"
     gene            complement(2464..2973)
                     /locus_tag="VF958_03285"
     CDS             complement(2464..2973)
                     /locus_tag="VF958_03285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9148162.1"
                     /translation="MKRIRTLSIGFLALVALAMFSPAAPGTNPAWEKMKSLVGEWEGT
                     MRHGEMDMPVKVSYELVSGGTSLMERMSAPDKDHEMITMYSPDGSRVMMTHYCSEGNQ
                     PRMRAEAVNGDTVAFSFVDATNLASPQAEHMRRLVVKFQDADHFTQEWTHRKAGKDET
                     GIFKYARKK"
     gene            complement(2970..3437)
                     /locus_tag="VF958_03290"
     CDS             complement(2970..3437)
                     /locus_tag="VF958_03290"
                     /inference="COORDINATES: protein motif:HMM:NF019928.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC domain-containing protein"
                     /protein_id="HEX9148163.1"
                     /translation="MATAQPIPETALRISRTFRARREKVFRAWTEPEALKRWFAPDDA
                     FDTKVLQFDPRPGGRYRIDMRKDDASHIVIGEYREVRPPERLVFTWRWENHPERGTED
                     TIVTLEFFDRGESTELVLTHERFPSAATRDDHNKGWTGCLDRLGRYISERSAS"
     gene            complement(3332..3775)
                     /locus_tag="VF958_03295"
     CDS             complement(3332..3775)
                     /locus_tag="VF958_03295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006225379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HEX9148164.1"
                     /translation="MVNYSGSLDRSFAALADPTRRAILARLALGEARVTDLARPFDIS
                     LPAVSKHLRVLEEAGLLKRRREGRVHHCRLDPKPLSTAADWIAHHRAFWEKSLDRLAE
                     YLSHELEEETTWQPRNRSPKRRSGSREPSGRAAKKSSAPGRNRKR"
BASE COUNT          584 a         1301 c         1279 g          663 t
ORIGIN      
        1 cgcgcggacg acctcctcga ggtccgcggc cacccgggac atcatccgga gcgcgtcctc
       61 ctcgggcgag agcgacgggt tcgcctgaag cggttccccc cagacgttgc ggtagctgcc
      121 gtccgcgttg cgcaggaaga agccgggcag gacgtcgcat ccacagaatc gggcgagaag
      181 ggcgggcgag cgcagaaacg gcgtcggcct gccgaagaac gggacgatga cctgatcctc
      241 cggataggcc cggtcgacga gcagggccac cgcgcgtccc cggtcgaccg ccgaccgcac
      301 ccggaacgcc gtcgccatcg acgatccgat gtcgatcgac tcgacgccga gccgttcgcg
      361 cagctcgagc cgcatctcct ggacgttcgg gtcgagctcc ggctgtccga cgactgcggg
      421 aacgaggccg tggcgtcgca gcgtcaccgc acccatctcc cagttgccga tgtgtcccgt
      481 gacgagcagg aatccgcgcc cttcgcgccg gctcgccgcg agcacgcggg cgtcggcttc
      541 gaacgtcgtg atgaccggcg tgaacgcctc gctccgcagc cgccacacgt cgatcgtggc
      601 gcgaccgtac tcgaagaaaa ctcgccgcgc gagccgggac gcatcgccgt cggaagcaat
      661 ccgcgcgcgt tcgaagttct ccgccatccc gcggcgcgtc gaacgcagga gggtgaccgc
      721 gatcgagttg ccgacggtcg agatcgtctt caaaatggtc agcggcagcc atcgggcgcc
      781 gtagtgcaga ccgccataca cgatccgatt ggacaacccg tggctgaccc atttccgacg
      841 ggcgcggggc gtgccggggg cgcctccgcc ggttccagga gcttcgattg gggacattgc
      901 cggcgcaaag tctacaatcc cgcgacgcca tgaacgttct gcttctccgg cccgatccgg
      961 gcaacgagcg tttcgggctg ggtccgttct tccgcgtcga gccgctgggg ctcgagtacg
     1021 tcggcgccgc cctccgcggt cggggtcacg agacgaccgt ggtcgatctg cggttcggcc
     1081 accgggtctc ctcgtcggtc caacggaccc ggccgcggat cgtcgggatc gcctgtatgc
     1141 acgcgctcga gctcgacgag gtcctcgcga cggcgcgcga ggtccgccgg gtcgcgccgg
     1201 acgctttcat cgtcgtcgga gggcacgccg ctgcttccta tccggctccg ctcgaacggc
     1261 cggagatcga cgccatctgc gtcgatgacg gcgaagaggt cgtccctgcc ctcgcggacg
     1321 ccctcgcgcg cggacgtccc gcctcggagg tgcccgctct ccggctctcg accccggacg
     1381 gttgggtctc gacgccggcg ctcgcggagc ggaccgccct cgatcgcatc gccttgcccg
     1441 accgctccct ggtcgatcgt gatcgcaatc gctaccactg cctcctcttc aagcccgtat
     1501 ggctggtcga gacggcgcgc ggctgcccgt tccgctgcag cttctgctcg gtctggcagc
     1561 tctacgaccg atcattccgc gaacgatcga tcggcgcggt cgtcgacgac ctcgcgtccg
     1621 tcggcgagaa cgtcttcatc gccgacgacc tcttctggaa tcacccggag cgcagcctcg
     1681 cgctcgcgga ggcattgaag aagcgcggcg tgaagaagcg ctggctactc gtgcagacgc
     1741 ggaccgacct cgtgtgccgg cggccggatc tgctcgaggc gtggaggcca atcgcgaagg
     1801 acttcgacat cttcttcggg ctcgaggcgg cgtcggacga agggctcgcg ggagtggtga
     1861 aagacacagg cgtatgggcg tcgatcgagg cggctcggat ttcgcgctcc ttcgggtacg
     1921 gcgtcaccgg caacttcctc gtggatcccg actggaacga ggatcgcttc cgcgagctct
     1981 gggcgttcgt cgcgcggtac ggcttcgaaa gggccggtta cacgatcctg acgccgctcc
     2041 ccgggaccga gctgtacgag cggctgcgac ccgtcatcgc gggacagccg tggttcaagt
     2101 acgacatgca ccacgtcctg tgggaacccc ggctcggcgc ccggcgcttc ttcgagctct
     2161 acgcggagac gtggcgccgc tcgatcctga acacggcggg ggagaagagc ctcttcgact
     2221 ggatgcgcca ggtgcggccg tcgcagatcc cgtacatcac ccgcgtgctg ctgcgcaccc
     2281 agcggatgat gcgccccgag gcgtacctga gggagcacga agcggcggag cgagtcgccc
     2341 gggagatccc cgccgagccg gcgcgcgcga cgtaggtcct gcccctcgac cgcggcgcga
     2401 aaaaggtctc cctcttcccg cgcgcgagga gagggtcggg ttgaggagtt gaagtccgcc
     2461 tctctacttc ttcctcgcgt acttgaagat ccccgtctcg tccttgccgg ccttgcggtg
     2521 cgtccactcc tgcgtgaagt ggtcggcgtc ctggaacttc acgacgagcc tgcgcatgtg
     2581 ctcggcttgc gggctcgcga ggttcgtcgc gtcgacgaac gagaaggcaa cggtgtcgcc
     2641 gttgacggct tccgctcgca tgcgcggctg atttccctcc gagcagtagt gggtcatcat
     2701 gacccgcgag ccgtccgggg agtacatcgt gatcatttcg tgatccttgt cgggcgcgct
     2761 catccgttcc atcagcgacg ttccgcccga gacgagctcg taagagacct ttaccggcat
     2821 gtccatctcg ccgtgtctca tcgtcccttc ccactcgccg acgagcgact tcatcttttc
     2881 ccacgccggg ttcgtgccgg gcgcggcggg gctgaacatg gcgagcgcga cgagggccag
     2941 gaatccgatc gagagcgttc gaatccgttt catgaggcac tcctttccga aatgtaacgg
     3001 ccgagccggt cgaggcagcc ggtccaaccc ttgttgtggt cgtcgcgagt ggccgcgctg
     3061 ggaaatctct cgtgggtcag caccagctcc gtcgactcac cccgatcgaa gaactcgaga
     3121 gtgacaatcg tatcttcggt gcccctctcc ggatggttct cccacctcca cgtgaagacg
     3181 agcctctccg gcgggcgcac ctcgcggtac tcgccgatga cgatgtggct ggcgtcgtcc
     3241 ttccgcatgt cgatgcgata gcgccccccg ggacgggggt cgaactgaag gactttcgtg
     3301 tcgaaggcgt cgtcgggcgc gaaccagcgc ttcagcgctt ccggttccgt ccaggcgcgg
     3361 aagacttttt cgcggcgcgc ccggaaggtt ctcgagatcc ggagcgccgt ttcggggatc
     3421 ggttgcgcgg ttgccatgtc gtttcctcct cgagctcatg gctcaggtac tcggcgagtc
     3481 ggtcgaggct cttttcccag aaggcgcggt ggtgcgcgat ccagtcggcg gcggtcgaaa
     3541 gcggtttcgg atcgagacgg cagtggtgga cccgcccctc gcggcggcgc ttgaggagtc
     3601 ccgcctcttc gagaacccgg aggtgcttcg agacggcggg cagcgaaata tcgaacggcc
     3661 tggcgaggtc ggtgacccgg gcctctccga gcgccagccg cgcgaggatg gcccgccggg
     3721 tcgggtccgc aagggccgcg aaggagcgat cgagagaccc ggaatagtta accattaagt
     3781 taactattgc tcggatccat ccccgctgtc aagcccacct cgccccg
//
LOCUS       DASWAV010000165         1737 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U2665
            k141_288964, whole genome shotgun sequence.
ACCESSION   DASWAV010000165 DASWAV010000000
VERSION     DASWAV010000165.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960316
            Sequence Read Archive: SRR10260007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 1737)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1737)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:47:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,929
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1737
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_288964"
                     /isolate="SMAG_U2665"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.36444 N 132.31889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1276)
                     /locus_tag="VF958_03300"
     CDS             complement(<1..1276)
                     /locus_tag="VF958_03300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9148165.1"
                     /translation="MEKLDRRDVRFLIACVAVMVIGGAVTALLFKRAFPEASIEFRVN
                     RSQARAEGEKFLHGLGRDLARTRFAGRFEIEEEPKVYLERELGLERAGRFYGRDAKVW
                     RWKMRWFRSGVKEEERVEITPRGDLAGYESVRREDAPGARLSKEEARARSLGFLERIG
                     IPAASLKPIEVSPVTRPKRTDWIFVDEKPSFVMGEATVRYSTTVAGAEVTGFHELVHV
                     PESWSRDYRRLRSKNEAAGTVATFGFFITLIAMLGVLITKIARRDVRWKVVAAFGAIA
                     FVLAFLSTLNEIPLTLYDYETSSSLSSHLTRQLVLGLLGAIATGAAIAVVVASAEPIY
                     RERFPRQLSLSGMFSRRGLRTKRFFLSVVLGYALTAFFFAYQAVFYVVAARFGAWAPA
                     DTPYRDLLNTALPWATVLLIGFLPAVSEEGISR"
     gene            complement(1288..>1737)
                     /locus_tag="VF958_03305"
     CDS             complement(1288..>1737)
                     /locus_tag="VF958_03305"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020260535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX9148166.1"
                     /translation="GDLEVWKRTLDVNVLGAHVVTDAAIPHLERERGSIVFVSSTAGQ
                     RGEARHSAYAASKGALISYTKSLAAELGPRGIRVNCVAPGWVDTEMTARALGDPDARA
                     EIERAIPLGRVARPGDVAGAILFLVSDLARHVQGEILNVNGGSVLAG"
BASE COUNT          298 a          598 c          585 g          256 t
ORIGIN      
        1 tccggctgat cccctcttcg gagacggcgg gcaggaagcc gatgaggagc accgtcgccc
       61 agggcagcgc ggtgttcagc aggtcgcggt acggggtgtc cgcgggcgcc cacgcgccga
      121 accgcgcggc gaccacgtag aagaccgcct gatacgcgaa gaagaacgcg gtcagcgcgt
      181 atccgaggac gaccgagagg aagaagcgct tcgtgcgcag gccgcggcgc gagaacatcc
      241 cggagagcga gagctggcgc gggaaacgct cccggtagat tggctcggct gaggccacga
      301 cgacggcgat ggccgcgccc gtcgcgatcg ccccgagcag gccgagcacg agctgccggg
      361 tcaagtggct ggagagggag ctcgacgtct cgtagtcgta cagggtgagc gggatctcgt
      421 tcaacgtcga aaggaacgcg aggacgaagg caattgctcc gaacgcggcg acgaccttcc
      481 aacgcacgtc gcgtcgcgcg atcttcgtga tcaggacgcc gagcattgcg atcagtgtga
      541 tgaagaagcc gaacgtcgca acggtcccgg cggcttcgtt cttcgagcgc agccgccggt
      601 agtcgcgcga ccaggactcc ggcacgtgca cgagctcgtg gaaccccgtg acctccgcgc
      661 cggcgaccgt cgtcgagtag cgcacggttg cttcgcccat gacgaaagac ggcttctcgt
      721 cgacgaaaat ccagtcggtc cgtttcgggc gcgtcaccgg cgaaacttcg atcggcttca
      781 ggctcgccgc cggaatgccg atcctctcga gaaaaccgag cgaccgcgct cgggcctctt
      841 ccttcgatag ccgcgcgccg ggagcatctt cgcgccggac cgattcgtag ccggcgagat
      901 ctccccgcgg cgtgatctcg acccgctcct cttccttcac gcccgagcgg aaccagcgca
      961 tcttccagcg ccagaccttc gcgtccctgc cgtagaaccg gcccgctcgc tcgagaccga
     1021 gttcgcgctc gaggtagacc ttcggctctt cctcgatctc gaacctcccg gcgaaccggg
     1081 ttcgggcgag gtcgcgcccg agtccgtgga ggaacttctc cccttcggcg cgcgcctggg
     1141 agcggttcac gcggaactcg atcgacgcct cgggaaatgc acgcttgaag aggagcgccg
     1201 tgaccgcgcc gccgatcacc atgaccgcga cgcacgcgat caggaaacgg acgtctctcc
     1261 ggtcgagctt ttccattcgc cctccgatta cccggccagc acgctgccgc cgttgacgtt
     1321 caggatctcg ccctgcacgt ggcgggcgag gtcggaaacg aggaagagaa tcgcaccggc
     1381 gacgtcgccg ggcctggcga cgcggccgag cgggatcgcg cgctcgatct ccgcgcgggc
     1441 atcggggtcg ccgagagccc gcgcggtcat ctctgtatcg acccaacccg gagcgacgca
     1501 gttcacgcgg atcccccgcg gccccagctc ggcggcgagc gacttcgtgt aggagatgag
     1561 cgcccccttc gaggcggcgt aggcggaatg ccgcgcctcg ccgcgctgcc ccgcggtcga
     1621 ggagacgaag acgatcgagc ccctctcgcg ctccaggtgt ggaatcgcgg cgtcggtcac
     1681 gacgtgggca ccgaggacgt tgacgtcgag cgtccgcttc cagacctcca ggtcacc
//
LOCUS       DASWAV010000166         2715 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U2665
            k141_9751, whole genome shotgun sequence.
ACCESSION   DASWAV010000166 DASWAV010000000
VERSION     DASWAV010000166.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960316
            Sequence Read Archive: SRR10260007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2715)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2715)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:47:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,929
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2715
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_9751"
                     /isolate="SMAG_U2665"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.36444 N 132.31889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..554
                     /locus_tag="VF958_03310"
     CDS             <1..554
                     /locus_tag="VF958_03310"
                     /inference="COORDINATES: protein motif:HMM:NF045194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HEX9148167.1"
                     /translation="YVLYLGTLEPRKDVETLVTACERLWTRRRARPDLVLAGGAGWKS
                     LPLYRRIARSAFRDKIHVAGYASRSVAVDLYRAAEAFVYPSLAEGFGLPVAEAMACGT
                     PVVASDIESLREVAGDAAILVPPRDAARFAIEIERALEDEPTRESLRQAGPRRAALFT
                     WEAAAEATVAALEQAAGAARRTA"
     gene            559..1725
                     /locus_tag="VF958_03315"
     CDS             559..1725
                     /locus_tag="VF958_03315"
                     /inference="COORDINATES: protein motif:HMM:NF045194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HEX9148168.1"
                     /translation="MNVRRPVVGIDARKLKDFGIGSYIRNLLEAMSHRPEAERYRFRV
                     YARRADREAVPALADNFEFVEEESPGYSIAELTGFAFRLLRDRLDLFHATHYVLPPLR
                     SRAVVTIHDIIHLLYPQFLPNRAALVYARVMIRRALRRADRIITVSYNSKRDLVDYFG
                     IAPARVDVIYNGVSREFRPDLPEEERARVARKYALRRPYLLFLGGEKPHKNVQNVVRA
                     FAEARRKRPDLPHVLALAGPMPKNSARIDALIGALDVASAIARPGLIDEADLPGLIAG
                     SDALLYPTLYEGFGLPVTEAMACGTPVLTSSTSALQEIAGGYSYLVDPLDVDAIARGI
                     LALTTDPKVRSDFIELGRKRALDFSWDKAAERTLEVYAEALSGAGNRKSEIGDR"
     gene            1722..>2715
                     /locus_tag="VF958_03320"
     CDS             1722..>2715
                     /locus_tag="VF958_03320"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX9148169.1"
                     /translation="MRTAVVHDWLNGMRGGEKVLEAILPLVPDPTIFTLFHVPGSVSP
                     DIERYPIRASFLNRFPFARRRYRQYLPLFPRAVESFDLSGFDFVVSSSHCVAKGAIAQ
                     GRTPHLCYCHTPVRYAYEQFDVYFPRDRTRLWSLKKAVIDRLRDWDVATADRPTRYLA
                     NSSAVADRIRRHYGRNATVLHPPVDVEFFHGGNASRSDFLLAVGSLVPYKRYEVAIEA
                     ARRLDRPLVIVGKGPEERRLRALAPLSVRIVSDVGPEALRELYRTCHAFVQPGEEDFG
                     ISLVEALACGAPVAAFGRGGARDSVRDGENGFLASEGTAEGLAAAIERIGRARFD"
BASE COUNT          391 a          958 c          915 g          451 t
ORIGIN      
        1 gctacgtcct ctacctgggc acgctcgagc cgcgaaagga cgtggagaca ctggtcaccg
       61 cctgcgagcg gctctggacc aggcggcggg cgcgcccgga cctcgtgctg gccggcggcg
      121 ccggctggaa gtcgctcccc ctctatcgcc gcatcgcgcg gtcagcgttc cgggacaaga
      181 tccacgtagc gggatacgcg agccgctccg tggccgtgga cctctatcgg gctgcggagg
      241 cgttcgtcta tccgtcgctc gcggagggat tcgggctgcc ggtcgcggaa gcgatggcct
      301 gtggaacgcc cgtcgtcgcc tccgacatcg agtcgttgcg cgaagtcgcc ggcgacgcag
      361 cgatcctcgt gccgccccgg gacgcagcga ggttcgcgat cgagatcgaa cgcgcgctcg
      421 aggacgagcc gacgcgggag agtctgcggc aggccggccc gcgacgcgcc gcgctcttca
      481 cctgggaggc agccgccgag gcaacggtcg cggctctcga gcaggcggcc ggcgcggcgc
      541 gaaggacggc gtgaggttat gaacgtccgc cgtcccgtcg tcggcatcga cgcgcgcaag
      601 ctgaaggatt tcggcatcgg ctcctacatt cgcaacctgc tcgaagcgat gtcgcaccgg
      661 cccgaggccg agcgataccg gttccgcgtc tatgcccgcc gggcggaccg cgaggcggtg
      721 ccggcgctcg cggacaactt cgagttcgtg gaggaggagt cgccgggcta ctcgatcgcg
      781 gagctcacgg ggttcgcatt ccgactgctc cgcgaccgac tcgatctctt ccacgcgact
      841 cactacgtgc tgccgcccct ccggagccgc gccgtcgtga cgattcacga catcattcat
      901 cttctctatc cccagttcct gccgaaccgt gcggcgctcg tctacgcgcg cgtgatgatc
      961 cggcgcgccc tccggagggc cgaccgcatc atcaccgttt cctacaacag caagcgggat
     1021 ctggtggact acttcggcat cgcccccgcg cgcgtcgacg tgatctacaa cggcgtgtcg
     1081 cgcgagtttc gcccggacct cccggaagag gagcgggcgc gcgtcgcacg aaagtacgcg
     1141 ctccgccgtc cctacctcct ttttctcggc ggcgagaagc cgcacaagaa cgtccagaac
     1201 gtcgtgcgcg cgtttgccga ggcgcgccgg aaacggccgg accttcctca cgttctcgcc
     1261 ctcgccggcc cgatgccgaa gaacagcgcg cggatcgacg ccctcatcgg ggccctcgac
     1321 gtggcctccg cgatcgcgcg cccgggcctc atcgacgagg cggacctccc gggactcatc
     1381 gccggatccg acgcgctgct ctatccgacg ctctacgagg ggttcggcct gccggtgacg
     1441 gaggcgatgg cgtgcggcac tcccgtgctc acgtcgtcga cgtccgcgct gcaggagatc
     1501 gcggggggat actcctacct ggtcgatccg ctcgacgtcg acgcgatcgc gcgcggcatc
     1561 ctcgcgctca cgaccgaccc gaaggtacgc tccgacttca tcgagctggg gcggaagcgc
     1621 gctctcgact tttcctggga caaggccgcg gagaggacgc tcgaggtcta cgcggaggcg
     1681 ctttccggtg ccgggaatcg aaaatcggaa atcggggatc gatgaggacc gccgtcgttc
     1741 acgactggtt aaacggaatg cgcggcggcg agaaggtgct cgaggcgatt ctgcctctcg
     1801 tcccggaccc gacgatcttc acgctgttcc acgtgccggg ctctgtctcc ccggacatcg
     1861 agcgttaccc gatccgcgcg tcgttcctga accgtttccc cttcgcgcgg cggcgctatc
     1921 gacagtacct cccgctcttt ccacgcgccg tcgagtcgtt cgacctgtcc ggcttcgact
     1981 tcgtcgtgtc ttcctctcac tgtgtcgcga agggcgcgat cgcccaggga aggactcctc
     2041 acctctgcta ctgccacacg ccggtccgct acgcgtacga gcagttcgac gtctactttc
     2101 cgcgcgaccg gacgaggctc tggtcgctca agaaggccgt gatcgaccgg ctgcgggact
     2161 gggacgtggc gacggcggac cgccccaccc gctacctcgc gaactcttcg gcggtggccg
     2221 atcggatccg ccgccattac ggccgcaacg ccaccgtcct tcaccccccc gtcgacgtcg
     2281 agttcttcca cggcgggaac gcgtcccggt cggacttcct cctggcggtc ggatcgctcg
     2341 tgccgtacaa gcggtatgag gtcgcgatcg aggcggcccg gcggctcgac cgtccgctcg
     2401 tgatcgtggg aaaggggccg gaggagcggc ggctccgcgc gctcgcgcct ctgtccgtaa
     2461 gaatcgtgtc cgacgtcgga cccgaggcgc tccgggagct ctaccggacc tgccacgcgt
     2521 tcgttcagcc gggcgaggag gacttcggca tctcgctcgt ggaagcgctc gcctgcggtg
     2581 ctccggtcgc ggcgttcggc cgggggggcg cgcgggactc ggtccgcgac ggcgagaacg
     2641 ggttcctggc ttccgaaggc acggcggaag ggctcgccgc ggcgatcgag aggatcggac
     2701 gcgcgcgctt cgatt
//
LOCUS       DASWAV010000167         3972 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U2665
            k141_528702, whole genome shotgun sequence.
ACCESSION   DASWAV010000167 DASWAV010000000
VERSION     DASWAV010000167.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960316
            Sequence Read Archive: SRR10260007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 3972)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3972)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:47:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,929
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3972
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_528702"
                     /isolate="SMAG_U2665"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.36444 N 132.31889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1888
                     /locus_tag="VF958_03325"
     CDS             <1..1888
                     /locus_tag="VF958_03325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9148170.1"
                     /translation="PVDEINPAVPPPLRWIVERCLAKTPRDRYVSTRDLARDLQTIRD
                     NAASISSRSLTAALPIEPRRRGIPVAAVAALALAVGAVGALFVSQQRVSTPPQPEFRR
                     LTFRRGVVSRALFAPNGSILYTASWEGGPPRSYLALPESSGIDRPLESEVQFPLAYSD
                     DGSQVLVLVGTSRLSINESGTLAWWPALGGRARRILDNAGWADAAPRGRFLVVVRNLG
                     ADRVLELRSADGVLQRTLFRTPGAIWFARISPDEKQVAFIYYPFRLDSAGEVHVVAID
                     GSGSKALTPRFERCEGLDWSAKTGEIWFSASGATAQGSSTLWTVSPSGKLRPRHVLPD
                     FFSLQSISAAGDRLLLTSKEERVDLTVRRGAQDPHDFSWLGWSLVTDISPDGKLALFY
                     DGGPTEKTMGVWVRPLDGGDATRLGDGYPGKFSPDGKWVIGLTRPLSGPPQLVLIPVE
                     AGGIRRLSSAGADVSAPSFAGLSTLLFVRSEKGVSEVWRMETDGSGARSLGAQGCGAP
                     TASPSANSFVCVEGERREKLVLHLMDKSPGRRLYELPKEGEFIYARWNGSGDRIFAVT
                     RDRRLLTLDSSTGALIREDTIPVSGTPGQDRLIAAALDADATIQAYSMIRLSSNLYLA
                     SGIR"
     gene            1956..3047
                     /locus_tag="VF958_03330"
     CDS             1956..3047
                     /locus_tag="VF958_03330"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010593568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HEX9148171.1"
                     /translation="MTSLHDLETPVVVVDLDVVDQNIRRMAACAKESGARLRPHAKTH
                     KIPELARLQLAAGAVGISLAKVGEAEVFADAGFEDVFLAYPIIGPEKARRLLALSDRM
                     RLAIGADGVEGAASVGVIFGAARRRIDVLLKVDCGYHRVGVAPEKAAQIAKRIADLPG
                     VALRGVFTHAGHAYLAAGSAEIEEIARREGEILASAAASIRAEGVPVEEVSVGSTPTA
                     KTVLSVPGVTECRPGNYVYHDASQVSLGTCGIEDCAMTVLATVVSVPAPERAVLDCGS
                     KTLSSDPLRPRADGHGWILGRKSRLEKLSEEHGVVRVEPGETFRVGERVRVLPNHACV
                     VSNLHDRVTLVRGDEVEGDWAVAARGRVR"
     gene            3047..3526
                     /locus_tag="VF958_03335"
     CDS             3047..3526
                     /locus_tag="VF958_03335"
                     /inference="COORDINATES: protein motif:HMM:NF024212.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEX9148172.1"
                     /translation="MAAKARGLQAELKQRKPFHSPAQELSVGLLRTADVLRHVLADQV
                     RADGITLQQYNVLRILRGAGEEGLPTLEIAGRMIERAPGVTRLLDRLEKKGFAERRRC
                     SPDRRQILCFITTSGLRLLAKLDGRVTSAEEAFAQTLGKTAVRRVNRALETLRRQPA"
     gene            3587..>3972
                     /locus_tag="VF958_03340"
     CDS             3587..>3972
                     /locus_tag="VF958_03340"
                     /inference="COORDINATES: protein motif:HMM:NF016176.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HEX9148173.1"
                     /translation="MVAVAPLTPASTERAKTYVIDKTHSDVSFQVRHLVTKVRGRFAD
                     FDAEIRAVPEAPEESSVRFTIRSASVDTDLPDRDQHLRSGDFFDADKHPEITFISSRI
                     RSAGDDRYDVTGLLTLRGVAREMTLPV"
BASE COUNT          632 a         1313 c         1328 g          699 t
ORIGIN      
        1 gcccgttgac gagatcaatc cggcagtgcc gccgccgctg cgctggatcg tcgaaaggtg
       61 tctcgcgaag actcctcggg accgttacgt ttcgacgcgc gacctcgctc gcgatcttca
      121 gacgatacgg gacaacgccg cgagcatcag ctcacgcagt ctgaccgccg ctctcccaat
      181 cgaaccccgg cgccgcggca tcccggtggc ggccgtcgcc gctctcgcgc tcgccgtcgg
      241 tgcggtgggc gcgctcttcg tctcgcagca gcgggtgagc acgcctccgc agcccgagtt
      301 ccgacgtctc acgtttcggc gcggggtcgt gtcgcgagcg ctcttcgcgc cgaacgggtc
      361 gatcctctac acggcgtcgt gggaaggcgg tccgccccgt tcttatctgg ctcttcccga
      421 gtcttccggg atcgaccggc cgctcgagtc cgaggttcag ttcccgctgg cgtattcgga
      481 cgatgggtcg caggtcctcg tgctcgtggg gacctcgcgg ctctcgatca acgagagcgg
      541 gacgctggca tggtggcctg ccctcggcgg cagggcgcgc cggatcctgg acaacgccgg
      601 gtgggcggac gccgccccga gaggacgttt cctcgtggtc gttcgcaatc tgggggcgga
      661 ccgcgtcctc gagctgcgca gcgccgacgg cgtgttgcag cgcacgcttt tccggactcc
      721 gggagcgatc tggtttgctc gaatctctcc ggacgagaag caggttgctt tcatttacta
      781 ccccttccgg ctcgacagcg cgggtgaagt gcacgtcgtc gcgatcgacg gctccggctc
      841 gaaggctttg acgccaaggt tcgagcgttg cgaggggctc gactggagcg cgaaaacggg
      901 cgagatctgg ttctcggcgt cgggcgcgac ggcgcagggc agcagcactc tctggacggt
      961 cagtccttcg gggaagctcc gaccgcgcca cgtcctgccg gatttcttct cgctccagag
     1021 catttcggcg gccggagacc gcttgctcct cacatcgaaa gaggagcgag tcgacctgac
     1081 ggtgcggcgc ggggcgcagg atccgcacga tttttcctgg ctgggctgga gcctcgtgac
     1141 cgatatctcg ccagacggaa agctggcgct cttctacgac ggcggcccga ccgagaaaac
     1201 gatgggagtc tgggttcgcc cgctcgatgg aggcgatgcg acgcgcctcg gagacggcta
     1261 cccgggaaag ttctcgcccg acggaaagtg ggtcatcggg ttgacgcggc cgctctcggg
     1321 cccccctcag ctcgttctca tcccggtcga ggcaggcggg atacgccggc tctcttctgc
     1381 cggcgcggat gtttccgcgc cttctttcgc cggcttgagc acgcttctct tcgtacgctc
     1441 ggagaaggga gtgagtgaag tctggaggat ggaaaccgat ggcagcggag ctcgttcgtt
     1501 gggagcacaa ggctgcggcg cgccgacggc ttctccgtca gcgaactcct tcgtgtgcgt
     1561 ggaaggggag cgaagggaga agctcgtcct ccatctcatg gacaagagtc ccggccgcag
     1621 gctgtatgag cttccgaagg agggagagtt catctatgcc cgctggaacg gcagcggaga
     1681 tcggatcttc gcggtcactc gagaccggcg gcttctgacg ctggattcct cgacgggggc
     1741 gctcatccgg gaagacacga ttccggtcag cggtacgccc ggccaggatc gcttgattgc
     1801 ggcggcgctc gatgcggatg cgacgatcca ggcgtattcg atgattcggc tctcgtccaa
     1861 tctctatctc gcctccggca ttcgttgaaa tccgcggcaa agaggcttgt tgcgcggatt
     1921 ctgctcttgt caggtcgccg ctgtaggctc ccggagtgac ctctcttcac gacctggaga
     1981 cgccggtcgt cgtcgtcgat ctcgacgtcg tcgaccagaa catccgccgg atggcggcat
     2041 gcgcgaagga aagcggcgcg cgcctgcggc cgcacgccaa gacccacaag atcccggagc
     2101 tcgcacgcct gcagctcgct gccggcgcgg tcgggatttc gctcgcgaag gtcggcgagg
     2161 cggaagtttt cgccgacgcc ggtttcgagg acgtctttct cgcgtacccg atcatcggtc
     2221 cggagaaggc ccggcgcctg ctcgcgctct ccgaccgcat gcgcctcgca atcggcgcgg
     2281 acggcgtgga aggggcggcg tccgtgggag tgatctttgg cgctgcgcgc cgccgaatcg
     2341 acgttctgct caaggtggac tgcggctatc accgcgtcgg ggtcgcgccg gagaaagcgg
     2401 cacagatcgc gaagcggatc gccgacctgc ccggcgtcgc gctgcgcgga gtcttcaccc
     2461 acgcaggcca cgcctatctc gccgccggga gtgccgagat cgaggagatc gctcggcgag
     2521 agggggaaat tctcgcctcc gcggccgcgt cgattcgcgc ggaaggcgtt ccagtcgaag
     2581 aagtctcggt cggctcgacg ccgaccgcga agaccgtcct gtcggtcccg ggcgtcaccg
     2641 agtgccggcc gggcaactac gtgtaccacg acgcctcgca ggtctcgctc ggaacgtgcg
     2701 gcatcgaaga ctgcgcgatg accgtcctcg ccacggtggt ttccgttcct gctcccgaac
     2761 gcgcggtcct cgactgcggc tcgaagactc tctcttccga ccccctgcgc ccgcgcgccg
     2821 acgggcacgg gtggattctc ggccggaaga gccgactcga gaagctctcg gaggagcatg
     2881 gcgtcgtccg cgtcgagccg ggcgagacgt tccgcgtcgg cgagagagtc cgcgttctcc
     2941 ccaaccacgc gtgcgtcgta tcaaatctcc acgaccgcgt gactctcgtt cgcggcgacg
     3001 aagtcgaggg cgattgggcg gttgcggcgc gcgggagagt gcggtgatgg cggcaaaggc
     3061 gcgagggttg caggccgagc tcaagcagcg aaagccgttt cactccccgg cccaggagct
     3121 ctccgtcggc ctcctgcgca ccgcggacgt cttgcgccac gtcctggctg accaggtccg
     3181 ggccgacggg atcacgctgc agcagtacaa cgtcctgagg atcctgcggg gcgccggcga
     3241 agaaggcctc cccacgctcg agatcgccgg ccggatgatc gagcgcgcgc ccggcgtgac
     3301 ccggctgctc gaccggctcg aaaagaaagg attcgccgag cgccgccgat gctcgccgga
     3361 ccggcgccag atcctctgtt tcatcacgac cagcgggctt cgcctcctgg cgaagctcga
     3421 cggacgggtc acgtcggccg aggaggcttt cgcccaaacc cttggaaaga cggccgttcg
     3481 gcgggtgaac cgagccctgg aaacccttcg ccgccagcct gcgtaattcc aacgtcgaga
     3541 ttagatgatc agtcaactat ttagagatca aggaaggagt caccccatgg tcgccgtcgc
     3601 ccccctcact cccgcttcca ccgaaagagc gaagacctac gtgattgaca agacgcattc
     3661 cgacgtctcg tttcaggtcc gtcacctcgt gacgaaagtg cgcggtcggt tcgccgactt
     3721 cgacgccgag atccgggcgg ttcccgaggc gcccgaggag tcctcggtgc gattcacgat
     3781 ccgaagcgct tccgtcgaca ccgacttgcc cgaccgggat cagcacctgc gctcgggcga
     3841 ctttttcgac gccgacaagc atccggaaat caccttcatc agcagccgaa tccgatcggc
     3901 cggcgacgat cggtacgacg tcaccggact tctcacactc cggggagtcg cgagagagat
     3961 gacgcttccc gt
//
LOCUS       DASWAV010000168         2026 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U2665
            k141_170392, whole genome shotgun sequence.
ACCESSION   DASWAV010000168 DASWAV010000000
VERSION     DASWAV010000168.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960316
            Sequence Read Archive: SRR10260007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2026)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2026)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:47:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,929
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2026
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_170392"
                     /isolate="SMAG_U2665"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.36444 N 132.31889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            87..800
                     /locus_tag="VF958_03345"
     CDS             87..800
                     /locus_tag="VF958_03345"
                     /inference="COORDINATES: protein motif:HMM:NF018712.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1326 domain-containing protein"
                     /protein_id="HEX9148174.1"
                     /translation="MKKYIPYLAVLAIAVAVVAATPADKSKTFSVTADTIEGCSCPLF
                     CTCYFSKSADEHMCQFNNVYRFRPGSHYGNVDLSNQLMWMSGDLGGEWHHKPGPGMPT
                     NWVVITLDKKSTPEQKKALEAIANASVFNTTWKKYSVREDTIEWHDEAKMSHAKLGSG
                     MAEITLNKFIGPDKKTPTVVENLQYWFTNSNKGFVLANSTHHFDGETKFSHKDRNGFN
                     ITWSAQGDVKPMATKAAMP"
     gene            931..1485
                     /locus_tag="VF958_03350"
     CDS             931..1485
                     /locus_tag="VF958_03350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008360938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase family
                     protein"
                     /protein_id="HEX9148175.1"
                     /translation="MTPDEIAPEFLTSAKSYAKAAPIEGVRTVKLPRFTDDRGFFLEV
                     FRARANHDGSRELAEFFSGVPVAQVNFSIVDAEGHIKGLHYHLKQDDIWFFPPPSKAK
                     IVLYDVRKDSPSFGRTQVVVAGGGQDVLVRIPAGVAHGYRPLTNPCSLLYFVTETFDP
                     DSPDEFRIPWDHPAVKDLWEVTNG"
     gene            1528..>2026
                     /locus_tag="VF958_03355"
     CDS             1528..>2026
                     /locus_tag="VF958_03355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010048903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HEX9148176.1"
                     /translation="MSSLPLPFDAARIPRVDAVGIEERAASLGKRSIKKAAKVAGIKL
                     AISMLDLTTLEGKDSEGKVRQLCQKALRPQPGDPSVPHVAAVCVYPNLVAIAKDALAG
                     SGVKVASVASGFPAGLVPLSAKLDDTRMAVEAGADEIDMVIDRGAFLSGDYEKVASEI
                     VAIKDA"
BASE COUNT          395 a          669 c          622 g          340 t
ORIGIN      
        1 tacacccggc cggctgccgt gcggtagaat ccccggccgc tgattcaaat gtttcaggac
       61 atgacccacc gaaaggaggt aactgcttga agaaatacat cccgtatctc gccgtgctcg
      121 cgatcgccgt cgccgtcgtc gccgcgaccc cggcagacaa gtcgaagacg ttctcggtca
      181 cggccgacac gatcgaaggc tgctcctgcc cgctcttctg cacgtgctac ttcagcaaat
      241 cggcggacga gcacatgtgc cagttcaaca acgtgtacag gttccgcccc gggagccact
      301 acggcaacgt cgatctctcg aaccagttga tgtggatgtc gggcgacctc ggcggcgagt
      361 ggcaccacaa gccgggacct ggcatgccaa cgaactgggt cgtgatcacg ctcgacaaga
      421 agtcgacgcc cgagcagaag aaggcgctcg aggcgatcgc caacgcctcc gtcttcaaca
      481 cgacgtggaa gaagtacagc gttcgcgagg acacgatcga gtggcacgac gaggccaaga
      541 tgtcgcacgc caagctgggt agcggcatgg cggagatcac gctgaacaag ttcatcggtc
      601 ccgacaagaa gacccccacc gtcgtcgaga acctccagta ctggttcacc aactcaaaca
      661 aggggttcgt gctggcgaat tcgactcatc atttcgacgg cgagacgaag ttcagccaca
      721 aggaccgcaa cgggttcaac atcacctggt cggcgcaggg tgacgtgaag ccgatggcga
      781 ccaaggcggc gatgccgtag ccgaacgaga tctctttttc acgaaacggg cgggcgaccg
      841 cccgtttttt ttattcgccg aaggttgcac cctctccctc tgggagagtg ggccgggggt
      901 gagggcgctg cctcgtctac aatctcctcc gtgaccccag acgagatcgc gccggagttc
      961 cttacctccg cgaagagcta cgcgaaggcc gcgcccatcg agggcgtgcg gaccgtgaag
     1021 ctcccgcgct tcacggacga ccgcggtttc tttctcgagg tgttccgtgc gcgcgcgaac
     1081 cacgacggga gccgggagct cgcggagttc ttctccggag tcccggtcgc gcaggtgaac
     1141 ttctcgatcg ttgacgccga agggcacatc aagggactcc actaccacct gaagcaggac
     1201 gacatctggt tctttccacc gccctccaag gcgaaaatcg ttctctacga cgtccgcaag
     1261 gactcgccgt cgttcgggcg gacgcaggtc gtcgtcgcgg gcggcgggca ggacgtcctc
     1321 gtccgcatcc ccgccggcgt cgcgcacggc taccgcccgc tcacgaaccc ttgctcgctg
     1381 ctctacttcg taacggagac gttcgacccc gactcgcccg acgagttccg catcccgtgg
     1441 gaccacccgg ccgtgaaaga cctctgggaa gtgaccaacg gctgaatcga gcccggtgcc
     1501 acgcgaccta taattgacgg ctcgaaaatg agctctctgc cccttccttt tgacgccgcg
     1561 cgcatccctc gcgtcgacgc cgtggggatc gaagaacgcg cggcctccct cggaaaacgc
     1621 tcgatcaaga aggctgcgaa ggttgccggg atcaaactgg cgatctcgat gctcgacctg
     1681 acgaccctcg agggcaagga ctccgagggc aaggtccggc agctctgcca gaaggccttg
     1741 cgcccgcagc cgggcgaccc ttcggtgccc cacgtcgcgg cggtgtgcgt ctacccgaac
     1801 ctcgtcgcga tcgcgaagga cgcgctcgcg ggaagcggag tcaaggtcgc ttcggtcgca
     1861 agcggctttc cggccgggct cgtgcccctg tccgcgaagc tcgacgacac gcggatggcg
     1921 gtcgaggccg gcgccgacga gatcgacatg gtcatcgacc gcggagcgtt cctctccggt
     1981 gactacgaga aggtcgcctc cgagatcgtc gcgatcaagg acgcct
//
LOCUS       DASWAV010000169         2376 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U2665
            k141_289323, whole genome shotgun sequence.
ACCESSION   DASWAV010000169 DASWAV010000000
VERSION     DASWAV010000169.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960316
            Sequence Read Archive: SRR10260007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2376)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2376)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:47:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,929
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2376
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_289323"
                     /isolate="SMAG_U2665"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.36444 N 132.31889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            59..1042
                     /locus_tag="VF958_03360"
     CDS             59..1042
                     /locus_tag="VF958_03360"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015836471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0033818 -
                     beta-ketoacyl-acyl-carrier-protein synthase III activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HEX9148177.1"
                     /translation="MKRARVAGTGAAVPKKVLTNADLEKLVDTSDEWIMTRTGIKERH
                     IVSEGEKFSDLCTKAADQALKSAHLKPEDLDMILVGTISSDMLFPATSCLVQRNLGAT
                     NAACSDLSAACVGFLYGLHMADALVQSNKADNILVIGGEILSRYVDWTDRSTCVLFGD
                     GAGAVVLQATKGDHGILGSLMKSNGCYADFICMPGGGSNRPANDATQIAEKLPYIKMK
                     GNETFKVAVKAMADVTAELLTEQGFTHEQIDLFIPHQANERIINAVGAKLKIAPGKVF
                     KNIERYGNTSAASIPIALDECVREGRIHDGDLILLTAFGSGLVWGSVLLRW"
     gene            1060..1974
                     /gene="fabD"
                     /locus_tag="VF958_03365"
     CDS             1060..1974
                     /gene="fabD"
                     /locus_tag="VF958_03365"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011554758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HEX9148178.1"
                     /translation="MGPLALLFPGQGSQFVGMGRALVESSPTARAVFDEADAALGFPL
                     SRLCFEGPEEDLKRTANTQPAILTHSVAALAELSARFPGRLEGAAFAAGHSLGEYSAC
                     VAAGVITFSDAVRLVRRRGELMQEAVPTGMGAMAAIVGLAAEDVEAACREAAQGEIVS
                     AANYNSPEQTVIAGHAGAVERASAACIAKGAKRAIPLPVSAPFHSPLMRPARERMEEL
                     LAATSFSDAGIPVVTNVDALPVRDGAKLRDSLVRQIDSPVRWVESVRRLVAEGVDRAL
                     EIGPGNVLAGLLRRIDRSIRVESWGGAP"
     gene            1971..>2376
                     /locus_tag="VF958_03370"
     CDS             1971..>2376
                     /locus_tag="VF958_03370"
                     /inference="COORDINATES: protein motif:HMM:NF024748.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008800 - beta-lactamase activity
                     [Evidence IEA];
                     GO_process: GO:0030655 - beta-lactam antibiotic catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HEX9148179.1"
                     /translation="MRSTLRVLGVLFTAALSGACASAPARVVASRSPIDAAVDAGAKL
                     CSCRIGVAARHIESGLAYERDAGAEFESASVIKIAVLTEVMAGVREGRIDLSERWALT
                     SSNKASESGWLRFFDAGLAPTWNDLVTLMIGPS"
BASE COUNT          378 a          801 c          811 g          386 t
ORIGIN      
        1 gtcgattgcc gagctgcacg cgcgggaagc ttcgctcctc aaagcttaag atcgagtcat
       61 gaagcgagcg cgcgtcgccg gcaccggagc tgccgtaccc aagaaggttc tgaccaacgc
      121 cgacctcgag aagctcgtcg acacgtccga cgagtggatc atgacccgga ccggcatcaa
      181 ggagaggcac attgtctcgg agggagagaa gttctccgac ctctgcacga aggccgccga
      241 ccaggcgctg aagagcgcgc atctcaagcc cgaagacctc gacatgatcc ttgtcgggac
      301 aatttcgagc gacatgctgt tcccggcgac gtcctgcctc gtccagcgca acctcggagc
      361 gacgaacgcg gcgtgctccg atctctccgc ggcctgcgtc ggattcctct acggcctgca
      421 catggcagac gccctcgttc agtcgaacaa ggcggacaac atcctcgtca tcggcggcga
      481 aatcctctcg cggtacgtcg actggacgga ccgctcgacg tgcgtgctgt tcggcgacgg
      541 ggccggcgca gtggtcctcc aggcgacgaa aggggaccac gggatcctgg gaagcctcat
      601 gaagtcgaac ggctgctacg cggacttcat ctgcatgccg ggcggcggct cgaaccggcc
      661 ggccaacgac gcgacgcaga tcgcagagaa actgccctac atcaagatga aggggaacga
      721 gacgttcaag gtcgcggtca aggcgatggc cgacgtgacc gccgaacttc ttactgagca
      781 gggattcacc cacgagcaga tcgacctttt catcccgcac caggccaacg agaggatcat
      841 caacgccgtc ggggcgaagc tgaagatcgc gcccggcaag gtcttcaaga acatcgagcg
      901 ctacgggaac acttcggcgg cgtcgattcc aatcgccctc gacgagtgcg tgcgcgaggg
      961 aagaattcac gacggggacc tgatcctcct gaccgccttc ggctccgggc tcgtgtgggg
     1021 ttcggtcctt ctccgctggt agcgggagcc gccgccgcat tgggtccgct cgctctcctc
     1081 ttccccggcc aggggtcgca gttcgtcggg atggggcggg cgctcgtcga gagctctccg
     1141 acggcgcgcg ccgtgttcga cgaggcggac gccgcgctcg gctttccgct ctcccgtctc
     1201 tgcttcgaag ggccggagga ggacctgaaa cgcaccgcca acacgcagcc ggcgatcctc
     1261 acgcactcgg tcgcggcgct cgcggagctc tcggcccgct ttcccgggag gctcgagggc
     1321 gcggcgttcg cggccggcca ctcgctcggc gagtactcgg cgtgcgtggc ggcgggcgtc
     1381 atcaccttct ccgacgccgt gcgcctggtc cggcggcgcg gagagctcat gcaggaggcg
     1441 gttcccaccg ggatgggcgc gatggcggcg attgtcgggc tcgccgcgga agacgtcgag
     1501 gcggcgtgcc gcgaagccgc gcagggcgag atcgtctcgg ccgccaacta caactctccc
     1561 gagcagaccg tgatcgcggg acacgcagga gccgtcgagc gcgcctcggc ggcgtgtatt
     1621 gcgaaggggg cgaagcgcgc gatcccgctg ccggtctccg cgccgtttca ctcgccgctc
     1681 atgcgtcccg ctcgtgagcg aatggaggaa ctgctcgcgg ccacgtcctt ctccgacgcc
     1741 gggattccgg tcgtcaccaa cgtggacgcc ctccccgtcc gggacggcgc gaagcttcgc
     1801 gattcgctcg tccggcagat cgattcgccg gtgaggtggg tcgagagcgt gcgtcggctc
     1861 gtggcggagg gtgtcgaccg cgcgctcgag atcggacccg gcaacgtgct cgccggcctc
     1921 ctccggcgga tcgaccgctc gatccgcgtc gagtcctggg gcggcgcgcc gtgaggtcga
     1981 cccttcgcgt actcggcgtg ctgttcacgg ccgcgctctc gggcgcttgc gccagcgctc
     2041 ccgcccgggt cgtggcgtcc cggtccccga tcgacgcggc cgtcgacgca ggcgcgaagc
     2101 tttgctcgtg ccggatcggc gtcgccgccc ggcacatcga gagcggtctc gcgtacgagc
     2161 gagacgcggg tgccgagttc gagtccgcat ccgtgatcaa gatcgcggtg ctgaccgaag
     2221 tcatggcggg cgttcgggaa ggacgcatcg acctgtccga gcgctgggcg ctgacctctt
     2281 cgaacaaggc ttccgagtcc ggatggcttc gcttcttcga cgcaggcctt gctcccacgt
     2341 ggaacgacct cgtgacgctg atgatcgggc cgtcgg
//
LOCUS       DASWAV010000170         3137 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U2665
            k141_410501, whole genome shotgun sequence.
ACCESSION   DASWAV010000170 DASWAV010000000
VERSION     DASWAV010000170.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960316
            Sequence Read Archive: SRR10260007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 3137)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3137)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:47:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,929
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3137
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_410501"
                     /isolate="SMAG_U2665"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.36444 N 132.31889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1632)
                     /gene="secA"
                     /locus_tag="VF958_03375"
     CDS             complement(<1..1632)
                     /gene="secA"
                     /locus_tag="VF958_03375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011734697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0017038 - protein import [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HEX9148180.1"
                     /translation="MIDRVLAKVFGTQHERDIKKLLPRVERINALEPAIQALSDEALR
                     GKTAELKARLAAALEGVPEEELKEARARELDGLLEEAFAVVREASVRALGMRPFDVQL
                     IGGMVLHQGKIAEMKTGEGKTLVATMPVYLNALSGRGVHVVTVNDYLARRDAEWMGRI
                     YRFLGLTVGCIQNSLLDDERKQQYAADVTYGTNNEFGFDYLRDNMKFELESMVQRGHI
                     YAIVDEVDSILIDEARTPLIISGPSEENTDIYYKCNRIIPRLVKGEETKDKYGNKTTI
                     GDYLVDEKSKTAVMTEEGVAKAEKLLGVKNLYDLTNIDLLHGTEQALRAHALYKKDVE
                     YMIKDGQVLIVDEFTGRVLPGRRWSDGLHQAVEAKEGVKIERENQTLATITLQNYFRL
                     YEKLAGMTGTADTEAGEFHQIYKLDVVVIPTNMPMIRIDHQDVVYASEREKFDAVADE
                     IAALREKGQPVLVGTVSIEKSERLSNLLKERKVPHVVLNAKFHEKEAEIVAQAGKSGS
                     VTIATNMAGRGTDIVLGGNPDAFADHRRTETKSAEE"
     gene            complement(1667..>3137)
                     /locus_tag="VF958_03380"
     CDS             complement(1667..>3137)
                     /locus_tag="VF958_03380"
                     /inference="COORDINATES: protein motif:HMM:TIGR00368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="YifB family Mg chelatase-like AAA ATPase"
                     /protein_id="HEX9148181.1"
                     /translation="LPHFTIVGLPDPSVREARERVKSALRNSGFTMPSGAVTVNLAPA
                     GFRKFGAALDLPVAVAFLEIAGLGPAKPMRRVFVGELGLDGQVRSIRGALCLALAAQE
                     RGFEEIVLPAANAAEAAALERIRVIPVRTLAATVEHLRGTRPIAPFVGARTNSEARGA
                     SDFADVKGQAVARRALEIAAAGGHNVLLVGPPGAGKTMLARRLPSILTPLTRAESIEV
                     TRIHSVAGTLPAGSGLVTAPPFRSPHHGTSAAGLLGGGPRPSPGEISLAHRGVLFLDE
                     LPEFRRDVIEGIRQPIEEKRVSIVRVGGACTFPCDFLLVAAMNPCPCGFLGDRRRACR
                     CDHPERARYARKLSGPLLDRIDLHVAVPAVPWEELESRSSETSAAMRERVSRARTRAA
                     ARRPDLPGFRNGDLSPGELEPISSLEPAARRLVAGAVERLGLSVRALHRSLKVARTVA
                     DLEGAAGIASAHVAEALSYRSRAEDREPGRPAFPDAAKT"
BASE COUNT          459 a         1029 c         1099 g          550 t
ORIGIN      
        1 ctcttcggcg gacttcgtct cggtgcgccg gtgatcggcg aacgcgtcgg gatttccacc
       61 gaggacgatg tcggttccgc ggcccgccat gttggttgcg atcgtcacgg agccggactt
      121 gcccgcctgc gcgacgatct ccgcctcctt ctcgtgaaac ttggcgttca ggacgacgtg
      181 gggaaccttg cgctccttga ggaggttcga gagtctctcg gacttttcga tcgagacggt
      241 gccgacgagc accggctggc ccttttcgcg gagcgccgcg atctcgtcgg cgaccgcgtc
      301 gaatttctcg cgctccgatg cgtagacgac gtcctggtgg tcgatgcgga tcatcggcat
      361 gttcgtcggg atcacgacga cgtcgagctt gtagatctgg tggaactcgc cggcctcggt
      421 gtccgccgtg ccggtcatgc ccgcgagctt ctcgtagaga cggaagtagt tctgcagggt
      481 gatcgtcgcg agcgtctggt tctcgcgctc gatcttcacg ccctccttcg cctccacggc
      541 ctggtggagt ccgtcggacc agcggcgccc gggcagcacg cggcccgtga actcgtcgac
      601 gatcaggacc tgcccgtcct tgatcatgta ctcgacgtct ttcttgtaga gcgcatgcgc
      661 gcggagcgcc tgctcggtgc cgtggagaag gtcgatgttc gtgaggtcgt agaggttctt
      721 gactccgagg agtttctccg ccttggccac gccctcctcg gtcataaccg ccgtcttcga
      781 cttctcgtcc acgaggtagt cgccgatggt cgtcttgttg ccgtacttgt ccttggtctc
      841 ttcacccttg accaggcggg gaatgatgcg gttgcacttg tagtagatgt cggtgttttc
      901 ctcggacggt cccgagatga tgagcggcgt gcgggcctcg tcgatcagga tcgagtcgac
      961 ctcgtcgacg atcgcgtaga tgtgcccccg ctggaccatg gattcgagct cgaacttcat
     1021 gttgtcgcgc aggtagtcga agccgaactc gttgttcgtc ccgtaggtga cgtcggcggc
     1081 gtactgttgc ttgcgttcgt cgtccaggag cgaattctgg atgcagccca ccgtcaggcc
     1141 gaggaaccgg tagatccggc ccatccactc ggcgtcgcgg cgcgccaggt agtcgttgac
     1201 ggtgacgacg tggacgccgc ggccgctgag ggcgttcagg tagacgggca tggtcgcgac
     1261 gagcgtcttc ccctcgcccg tcttcatctc ggcgatcttc ccctggtgca ggaccatccc
     1321 gccgatcagc tgcacgtcga agggccgcat gccgagcgcc cggaccgacg cctcgcggac
     1381 gacggcgaag gcttcctcga ggaggccgtc gagctcccgg gcccgggctt ctttcagctc
     1441 ctcctccggg accccctcga gggcggcggc caggcgggcc ttcagctccg cggtctttcc
     1501 ccgcagcgct tcgtcggaga gggcctggat cgccggctcg agcgcattga tccgctcgac
     1561 gcgcggcagg agcttcttga tgtcgcgctc gtgctgggtg ccgaagactt tggcgaggac
     1621 ccggtcgatc atttgggcca taaacagttc atgcggggag aattattcac gttttcgcgg
     1681 cgtccggaaa ggcggggcgt ccgggctccc ggtcctccgc ccgcgatcga taggagagcg
     1741 cctcggcgac gtgagccgac gcgatccccg cggccccttc gagatccgcg acggtgcggg
     1801 cgactttcag cgagcggtgc agcgcccgca ccgagagccc cagccgttcg accgcgccgg
     1861 cgacgaggcg cctcgccgcg ggctcgagag aggagatcgg ttcgagctcg cccggggaga
     1921 ggtccccgtt ccggaaaccg gggaggtccg gccgacgggc ggccgcgcgg gtgcgggccc
     1981 gggagacccg ctcgcgcatc gccgccgacg tctcggagga tcgactttcg agttcctccc
     2041 aggggaccgc cggaaccgcc acgtggaggt cgattcgatc gaggagcggt cccgagagct
     2101 tccgggcgta ccgcgcgcgc tcggggtgat cgcagcgaca ggcgcggcgc cggtcgccga
     2161 gaaacccgca cgggcacgga ttcattgcgg cgacgaggag gaaatcgcat gggaacgtgc
     2221 acgcgccacc cacccggacg atcgaaacgc gcttctcctc gatcggctgg cggatccctt
     2281 ctatgacgtc ccgccggaat tccggcagct cgtcgagaaa gagcacaccg cggtgagcga
     2341 gcgagatctc ccccggggaa gggcgcggtc ccccgccgag gaggcccgcc gcggaggtcc
     2401 cgtggtgggg agatcgaaag ggcggcgccg tgacgaggcc gctgccggcc gggagcgtcc
     2461 ccgcaaccga gtggatccgg gtcacttcga tcgattcggc gcgcgtcagg ggcgtcagga
     2521 tggaaggcag ccgccgcgcg agcatggtct ttccggcacc cggcggtccg accagcagga
     2581 cgttgtgccc gccggccgcg gcgatctcga gggctctccg cgcgacggcc tggcctttca
     2641 cgtcggcaaa atcgctcgct cctcgcgcct cggagttggt ccgcgcgccg acgaagggcg
     2701 cgatgggtcg ggttccgcgc aggtgctcga cggtcgccgc gagcgtccgc acgggaatga
     2761 cccggatccg ttcgagggcc gcagcctcgg cggcattggc cgccgggagg acgatttctt
     2821 cgaatccccg ctcttgagct gcgagcgcga ggcaaagagc gccgcgaatc gagcgaacct
     2881 ggccgtcgag gccgagctcg ccgacgaaca cccggcgcat cggtttcgcg gggccgagcc
     2941 cggcgatctc gaggaaggcg acggcgactg gcagatcgag ggccgccccg aacttgcgaa
     3001 agccggccgg agcgaggttg acggtgacgg cccccgacgg catcgtaaaa ccgctgttgc
     3061 gcagcgccga cttcacccgc tcacgagctt cgcggacgct cgggtcgggc aggccgacga
     3121 tcgtgaagtg gggaagc
//
LOCUS       DASWAV010000171         3276 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U2665
            k141_250534, whole genome shotgun sequence.
ACCESSION   DASWAV010000171 DASWAV010000000
VERSION     DASWAV010000171.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960316
            Sequence Read Archive: SRR10260007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 3276)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3276)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:47:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,929
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3276
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_250534"
                     /isolate="SMAG_U2665"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.36444 N 132.31889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(173..2107)
                     /locus_tag="VF958_03385"
     CDS             complement(173..2107)
                     /locus_tag="VF958_03385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020596102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate synthase"
                     /protein_id="HEX9148182.1"
                     /translation="MISGRPGDAHALQVRGDLTRSYGDVLTPDAMAALAALAPLNVPR
                     RALMTARIERRAARARNRQRIDFLDPRGTIPGTNITVKDAREGNFIGSEIPPDLQRQW
                     IQGTGPATRPNEPVEKGIRNVAYALLSGADGWMFDGEDALGQVSTMSLDNQRNLKLAI
                     ARDPVFMKVAERVASEMNGWARGFFGRPIIDDWERQLAFTTKMWRARGLHLDDRHVRL
                     ADGTGFSASIVDTVLYVVNNHERLKQSGASIVLYLPKIQTAEEAALWSRMFDSLEQHL
                     SLPSGAIKVYVLVEQIEACYQLMEIRAALGRHFVGFNTGRWDYINSVSDAMAWDREFV
                     NPNIDAIVMTYGYMRNYEDRVRRACNTPDREGRFALWEGGMETNIPVGSEAGVASGMK
                     KAVAGAEREQREGASGKWVAHWKMVHIVRPVWEKVGQDNQLGRRFPALTYTEADSDAL
                     VLLEPAPRTVGGARDLLSVALQYGNAFGKGFQAAALKPADFFGNDDVLYLMEDMATGE
                     IRLSILWEWLHKAAAFTEGDAATGVAKGEKFGADLFARLLNEEYAKLRAASNRDVHDD
                     SKTTTLPIAREIVATYVTVPFKAPWYIDLLNLNLNNEDLAEAKRRIGQYMDAFGKSGV
                     RITENLDFEVASSTAGRSQP"
     gene            2440..3039
                     /gene="lexA"
                     /locus_tag="VF958_03390"
     CDS             2440..3039
                     /gene="lexA"
                     /locus_tag="VF958_03390"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012680633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HEX9148183.1"
                     /translation="MNLTKRQKEILDFIREYRDRNGISPTQREIRERFQLSSFGTVQK
                     HLKRLEEKGALSREWNRSRGISPAEPKSTSREVALLGAVAAGRPIEPFPDEETIEVPS
                     TLLGRGEHFVLRVRGDSMVDDGIRDGDYVVVSKRSTAQNGQTVVALIRGEATLKRYYA
                     EGSRVRLQPANAAMKPLMVDARDVTLQGVVTGLIRNYTV"
     gene            3150..3225
                     /locus_tag="VF958_03395"
     tRNA            3150..3225
                     /locus_tag="VF958_03395"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3183..3185,aa:Cys,seq:gca)
BASE COUNT          561 a         1038 c         1059 g          618 t
ORIGIN      
        1 gccatccggg ccacggtgta gtcggtggcg atggtgcccc gttgctcgac gacctggcgc
       61 gcggaataca agcgcgtgat gccttcgaag cggggactgg cgaaccatcg ggcagcctcg
      121 tcgacttccg aatcaaacgg gctcgtggct ggctcgtact ttgccgaagt actcatggct
      181 ggctcctacc tgcggtcgaa ctcgcgacct cgaaatccag gttttccgtg atgcggacgc
      241 cgctcttgcc gaaggcgtcc atgtactgac cgatccgccg cttcgcctcc gccaggtcct
      301 cgttgttgag gttgaggttg agcagatcga tgtaccaggg cgccttgaaa gggacggtga
      361 cgtacgtcgc gacgatctcg cgcgcgatcg gcaacgtcgt cgtcttcgag tcgtcgtgga
      421 cgtcccggtt gctcgcggcc cgaagcttgg cgtactcctc gttgaggaga cgcgcaaaga
      481 gatcggcgcc gaacttctcc cccttcgcga cgccggtcgc cgcgtcgccc tcggtgaacg
      541 cggctgcctt gtgcagccac tcccagagga tggaaagacg gatctccccg gtcgccatgt
      601 cctccatgag atagaggacg tcatcgttgc cgaagaagtc ggccggcttc aacgccgccg
      661 cttgaaatcc cttgccgaag gcattgccgt attgaagggc gacgctcagg agatcccgcg
      721 ccccgccgac ggtgcgcggc gccggctcga gcaggacgag ggcgtcggaa tcggcctccg
      781 tgtaggtgag cgcggggaaa cgccggccga gctgattgtc ctgacccacc ttctcccaga
      841 ccggcctcac gatgtgcacc atcttccagt gggcgaccca cttgccgctc gcaccctcgc
      901 gctgctcgcg ctctgcgccg gccacggctt tcttcattcc gctcgcgacg ccggcttccg
      961 agcccaccgg gatgttggtc tccatgccgc cttcccacag ggcgaagcga ccctcgcggt
     1021 caggggtgtt gcaggcccgc cgcacgcggt cctcgtagtt ccgcatgtat ccgtaggtca
     1081 tcacgatggc gtcgatgttg ggattgacga actcccgatc ccacgccatc gcgtcggaga
     1141 cgctgttgat gtagtcccag cggccggtgt tgaagccgac gaagtgccgc ccgagcgccg
     1201 cgcggatttc catcaactga taacaggcct cgatctgctc gacgagcacg tagaccttga
     1261 tcgcgccgga gggaagcgac aggtgctgct cgagggagtc gaacatgcgg ctccacagcg
     1321 ccgcctcctc ggcagtctga atcttcggca ggtagaggac gatcgaggcg ccggactgct
     1381 tcaaccgctc gtggttgttg acgacgtaga gcaccgtatc gacgatggac gccgagaatc
     1441 cggttccgtc ggcgaggcga acgtgccgat cgtcgaggtg aagtccccgc gcgcgccaca
     1501 tcttggtcgt gaaggcgagc tgcctctccc agtcgtcgat gatcgggcgc ccgaagaacc
     1561 cgcgcgccca cccgttcatt tcggaggcga cccgctcggc aaccttcatg aaaacgggat
     1621 cacgcgcgat cgcgagcttc aggttgcgct ggttgtcgag cgacatagtc gagacctggc
     1681 cgagcgcgtc ctcgccgtcg aacatccagc cgtccgcgcc cgagagcagc gcataggcga
     1741 cgttgcggat gcccttctcc accggctcgt tcggccgcgt cgcgggaccc gtgccctgga
     1801 tccactgccg ctggagatcg ggaggaatct cgctgccgat gaaattccct tcccgtgcat
     1861 ccttgaccgt gatgttcgtt ccgggaatcg tgccgcgggg gtcgaggaag tcgatgcgct
     1921 ggcggttgcg cgcgcgcgcg gcccgtcgct cgatgcgagc cgtcatcagc gctcttcgcg
     1981 gcacgttcaa gggggcgagg gcggcgagcg ccgccatcgc atccggggta aggacgtcac
     2041 cgtaggagcg cgtgaggtcg ccacgaacct ggagcgcgtg agcgtcgccc ggtctgccgc
     2101 tgatcatccg tgggtccccc gatccgaggt cgtgctggcg cagagaactg gggcccggag
     2161 tttatctccc gacgtttgat cgcgcccgat ccttacgatg gtgacggatt ctccgacttg
     2221 acttgcgcct ttcttccgcc taggattcct ttgtgaaggc cgatgggccg gctccggatg
     2281 gccacggagc ggccgcggtg gtccctcggc cttaaattat tcggttagca gagggagtac
     2341 cttaaaaaga atggaaatgc tgacacaact taccggtgat ccggatccta acaatattcg
     2401 gctaatccaa agaatctcct tagaaagaat ggaagggcga tgaatctgac aaagagacag
     2461 aaggagatcc tcgatttcat ccgtgagtac cgcgaccgca acggcatctc tccgacgcag
     2521 cgcgaaatcc gtgagcggtt ccagctctcc tccttcggaa cggtgcagaa gcacctgaag
     2581 cgcctcgagg agaagggagc actctcgcgc gagtggaacc gcagccgcgg catctcgccg
     2641 gccgagccga agtcgacgtc gcgcgaggtc gcgctcctcg gagcggtcgc cgccggccgc
     2701 ccgatcgagc cctttcccga cgaggaaacg atcgaggttc cctcgacgct cctcggacgg
     2761 ggggagcatt tcgtcctgcg tgtccgcggg gactcgatgg tggacgacgg gatccgggac
     2821 ggcgactacg tcgtcgtgtc caagcgttcg accgcgcaga acggccagac cgtggtcgct
     2881 ttgattcgcg gtgaggcgac gttgaaacgc tattacgccg agggttcccg ggtccggctg
     2941 cagcccgcca atgcggcgat gaagcctctg atggtggacg cccgcgacgt gacactgcag
     3001 ggagtggtta ccgggctgat ccggaattac accgtttgac gagaagctaa gttcgcgttt
     3061 ttggcgcatg ggatggtgct ccggggccgt ttcctttctc gaaccgccgt cgaggcggtg
     3121 gcgacaggag ttaagatctt ccttctactg gcgacgtacc caagtggtta agggagaggt
     3181 ctgcaaaacc tttattcggc ggttcgaatc cgcccgtcgc ctccacttag acgccggccg
     3241 gtagctcgct ccctcgtcgc cggagtggcg gaattg
//
LOCUS       DASWAV010000172         3550 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U2665
            k141_767884, whole genome shotgun sequence.
ACCESSION   DASWAV010000172 DASWAV010000000
VERSION     DASWAV010000172.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960316
            Sequence Read Archive: SRR10260007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 3550)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3550)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:47:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,929
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3550
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_767884"
                     /isolate="SMAG_U2665"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.36444 N 132.31889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..3472)
                     /locus_tag="VF958_03400"
     CDS             complement(<1..3472)
                     /locus_tag="VF958_03400"
                     /inference="COORDINATES: protein motif:HMM:NF025006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HEX9148184.1"
                     /translation="MQRRTTVNVTLKRGGTIRGVVSDPNGKPAEGATVIVGSLAAKTD
                     TSGACELAGVPPGTQQVEVFWKEDLAARRDTLHVKKGETVDAALRLAKAASVTGSVID
                     EKTRRPIAGVRVSASSASLFREDARTRRARTDAKGRFRLAGLAARNYTVRASKTDYLP
                     VVMPGILAGASSPGTVAIALQKAAAVSGRVSDESGAPVSGARVRFARDSNIRAVMRGG
                     PAVFFARPGVTTGPEGTFRMRGLAAEKNLTLEAAKPGFVTAKRHGVTLKPGEAVKDLA
                     LILKRGLEARGKVVDSTGQAIPGAEIRVTPVERGGARFVAQFAGVNREKPDATSGPDG
                     SFHLAGLEAGEYALGVSRDGYAPKRVPSATVVEKGPNDWPPIILAAGVPIAGVVRNSK
                     GDPIVGAEVFAFGDGITRNSRTDPEGRFRMDGYGADRRVMINVRAEGYAALQRSVTPS
                     AEEVALVLTTRGTIRGRVEDGATNRPVTDFTASYTDGGGRLIGGIRVVMGMGDRENTF
                     QSPDGTFELSDVPPGKWNVRASAPGYRPAEVSGIELAEGETKEGVVVSLKKGGVVSGR
                     VLDPRRGTGVPNASVSWSEGSDPSSSSPGMMVLSRLDAGAGNAATTDADGRFRFDGLP
                     TGKITLSADHPDFLEVSKQVDLDEEATVDLTLALGGAISGTVVGRDGRSVIPGAEVFL
                     RDQGAAYSIGDDSARADASGNFSFEHLKAGRYSVSARSNAGATAWKDVVLAESQRQEG
                     VLLEITTGATVQGTVSGLPGRSLGGVRVFASTEDYQDSAVTGDDGRFTLRDVPAGVLR
                     LQASTAFPSMRSTSKNLDVPEGATEVPVEIVFEGASRLSGRITRGDKPVSSAFVSAVP
                     DPPTPTGGRVSSQTDEDGRYSIEGLVDGNYLVRVSGQSASYSRSFTVSGDTNGDIALP
                     TLTISGVVTDAGSNEPIEGAFIQAEMGQERTGLPARYTQTDSRGFYSLDGVDSGNYQV
                     TARKEGYQLKTLPVSVAAAPAELNVSLTRGAGLSIRAVDGLTGIPLRSLFVLAYAGSG
                     SLAFTGDVGLDGEGKGEISSLTPGAYSLYLFSAGYASRSYRVTVPSPMLSIAVTPGGR
                     LEVRTEAAFSGRIVDATGAPYMIFPGRLDARVTGAPPAVSWGGIAPGSYRLIVSGSSG
                     ESAFPFTVAEG"
BASE COUNT          546 a         1271 c         1163 g          570 t
ORIGIN      
        1 ggccctccgc cacagtgaac ggaaacgcgc tctcccccga tgaacccgag acgatcagcc
       61 gataggaacc gggcgcgatg cctccccacg agaccgcggg tggcgcgccg gttactcgcg
      121 cgtcgagcct tcccggaaag atcatgtacg gcgcacccgt cgcgtccacg atgcggccgc
      181 tgaaagccgc ctcggtgcgg acttccagcc gcccgcccgg tgtcaccgcg atcgagagca
      241 tcggggaagg aaccgtcacc cgataggaac gcgaggcata tcccgccgag aagagataga
      301 ggctgtaggc ccccggcgtg agcgaagaga tctccccctt cccctccccg tcgagaccga
      361 cgtctcccgt gaaggcgagg cttcccgatc ccgcgtaagc gagaacgaag aggctccgga
      421 gcggaatccc cgtgagcccg tcaaccgcgc ggatcgagag tccggcccca cgggtgagtg
      481 agacgttcaa ctcggcagga gcggctgcga ccgacacggg cagcgtcttg agctgataac
      541 cctccttgcg cgcggtgacc tggtagttcc cggagtccac gccgtcgagg gagtagaaac
      601 cccgcgagtc ggtctgcgtg taccgagccg gcaaacccgt ccgctcctgc cccatctccg
      661 cctggatgaa cgcgccttcg atcggttcgt tggagccggc gtccgtgacg accccggaga
      721 tcgtcagcgt gggaagcgcg atgtccccgt tcgtgtcgcc cgacaccgtg aagctgcggc
      781 tgtagctcgc gctctgtccc gagaccctca ccaggtaatt tccgtcgacg aggccctcga
      841 tcgaatatcg gccatcctcg tcggtctgtg acgaaacccg gccgcccgtc ggcgtcggcg
      901 gatccggcac cgcgctgacg aaggcgctcg aaaccggctt gtccccgcgg gtgatgcgac
      961 cggagagccg cgatgcgccc tcgaaaacga tctctaccgg gacctcggtt gccccctcag
     1021 gcacgtcgag gttcttgctc gtcgaccgca tcgaggggaa tgccgtgctc gcctgcagcc
     1081 gcagaacgcc ggccgggacg tcgcgaagcg tgaaccgtcc gtcgtcgccg gtcaccgcgc
     1141 tgtcctggta gtcctcggtg gacgcgaaaa cccgcacgcc tcccagggag cggcccggca
     1201 gacccgaaac ggttccctgg acggtggcgc cggtcgtgat ctcgaggagg acgccctcct
     1261 gccgctggct ctccgcgagg acgacgtcct tccacgcggt cgcgccggca ttcgagcgcg
     1321 ccgacacgct gtagcggccc gccttcaggt gctcgaagga gaagttgccg ctggcgtccg
     1381 cacgggcgga gtcgtcgccg atgctgtagg ccgcaccctg gtcgcgaagg aagacctccg
     1441 caccgggtat gacgcttcgc ccgtccctac cgaccacggt tcccgagatc gctcccccga
     1501 gggcaagcgt caggtccacg gtggcttcct cgtcgaggtc gacctgcttc gaaacttcga
     1561 gaaagtcggg atggtccgcc gacaacgtga tcttgccggt tggaagcccg tcgaagcgga
     1621 accggccgtc ggcgtcggtc gtcgcggcgt tcccggcccc cgcatcgagg cgcgagagga
     1681 ccatcattcc cgggctcgag gaagacggat cggagccttc agaccaggag accgaggcgt
     1741 tcggcacgcc ggtgccccga cgcgggtcga gcactctgcc ggagacaacg cctcccttct
     1801 tgagggagac gacgactcct tctttcgtct ccccctccgc gagctcgatc cccgatactt
     1861 cggcgggccg gtaccccggc gccgatgccc ggacgttcca ctttccgggg ggtacgtcgg
     1921 agagctcgaa ggtcccgtcg ggggactgga acgtattctc gcggtcaccc atccccatca
     1981 cgactcggat gcctccgatg agccttcccc caccgtcggt gtacgacgcc gtgaagtcgg
     2041 tcaccggacg gttcgtcgcg ccgtcctcga cgcgcccgcg gatcgtcccc ctcgtcgtca
     2101 ggacgagcgc gacttcttcc gcggaaggcg tcaccgaacg ctgcagggcc gcgtatcctt
     2161 cggcacggac gttgatcatc actctgcggt cggctccata tccgtccata cggaaccttc
     2221 cttcgggatc cgtgcgcgag ttgcgggtga ttccatcccc gaacgcgaac acctcggccc
     2281 cgacgatcgg atcacccttc gagttgcgca cgaccccggc gatgggaact ccggctgcga
     2341 ggatgatcgg cggccagtcg ttcggacctt tctcgacgac tgtggccgac ggcacgcgct
     2401 tcggcgcgta tccgtcgcgc gagacgccaa gagcatattc accggcttcg agacccgcca
     2461 ggtggaacga gccgtcgggg ccgcttgtgg cgtcgggctt ctcgcggttg accccggcga
     2521 actgggcgac gaaacgggcg ccaccgcgct ccacgggcgt cacgcggatc tccgcaccgg
     2581 ggatggcctg tccggtcgag tctacgacct ttccacgggc ctcgagccct cgcttcagga
     2641 tcagcgcgag gtccttgacc gcctcccccg gtttcagcgt gacgccgtgc cgcttcgcgg
     2701 tgacgaagcc gggcttcgcg gcctcgaggg tcaggttctt ctcggctgcg agtccacgca
     2761 tgcggaatgt cccttcggga ccggtcgtca cgccgggccg tgcgaagaag acggcaggac
     2821 cgccgcgcat caccgctcga atgttcgagt cccgcgcgaa acgcacccgt gctccggaga
     2881 ccggcgcgcc ggattcgtcg ctcacgcggc cgctgaccgc cgccgccttc tggagagcga
     2941 tggcgacggt ccccggcgaa gaggctccgg cgaggatccc cggcatgaca accgggaggt
     3001 agtcggtctt cgacgcgcgg accgtgtagt tgcgtgccgc gagacccgcg agccggaacc
     3061 ttcccttcgc gtcggtgcgc gcccggcggg tgcgagcgtc ttcgcggaag agacttgcgg
     3121 acgacgccga cacgcggacg cccgcgatgg ggcgccgggt cttctcgtcg atgacgctgc
     3181 ccgtcaccga cgccgccttc gccagacgca gcgcggcgtc gaccgtctcg cccttcttca
     3241 cgtggagggt gtcccggcgg gcggcgagat cctccttcca gaacacctcg acctgctgag
     3301 tcccgggcgg gacgcccgcg agctcacagg ccccggatgt gtcggtcttc gcagcgagcg
     3361 atccgacgat gaccgttgct ccctccgccg gctttccgtt gggatcgctg acgactcccc
     3421 ggatcgtccc gcctcgcttc agcgtgacgt tgaccgtcgt ccggcgctgc atcgacggct
     3481 gccgcgacgg cgagtacccg gctgcgcgta ccgagagtcc accgccgctc accggcgcgt
     3541 tggcgatgga
//
LOCUS       DASWAV010000173         2350 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U2665
            k141_689989, whole genome shotgun sequence.
ACCESSION   DASWAV010000173 DASWAV010000000
VERSION     DASWAV010000173.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960316
            Sequence Read Archive: SRR10260007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2350)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2350)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:47:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,929
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2350
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_689989"
                     /isolate="SMAG_U2665"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.36444 N 132.31889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            512..>2350
                     /locus_tag="VF958_03405"
     CDS             512..>2350
                     /locus_tag="VF958_03405"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HEX9148185.1"
                     /translation="MSMDRRNSHPKDNLAEIIGDEARLEALRRTSLLDSPPEEVFDRL
                     TRLATAVLHVPLAMVSLVDSDRQFFKSQCGLSEPLASLRETPLTNSFCKHAVGSREPL
                     IVADARRDPVFAQSADSSDVGVIAYAGVPLITSDGYALGTFCIVDQEPHDWTDEEIGI
                     LRVLATSTMSEIELRRLVGELRALTTNLQGVVEARTSELRASEERQRVLLEVNNAIVT
                     CLDRESLFRATTAALESVIPFDRAALHIYDPDKQVLRVFGVTGPVPLSPIFPIGTELP
                     RQQSRNGWVLDHGVPLITFDMRNPPPFLEHPALVQGGLRSAVSVPMTVKGKIIGTLHV
                     GSRVCGAYGADEASLLLAIAEQVGLAIENLLAYEEIAALKARLEEENVYLQEEVRTEA
                     AFGDVIGESPQILAVLAGVRKVAKTDSTVLVTGETGTGKELIVRAIHDLSRRKDKLLV
                     KVNCAALPAGVIESELFGHERGAFTGALTRKVGRFELANRGTLFLDEVGDLPLELQAK
                     LLRVLQEGEFERVGGTQTLKVDVRLIAATNRDLERAVSEGHFRADLYYRLNVFPIVIP
                     PLRKRLQDVPRLARHFAMLYASKMGKNVGPLNTDVLERLAAYAWPGN"
BASE COUNT          405 a          778 c          757 g          410 t
ORIGIN      
        1 ccacatgcag ggcactcgct tccgtcacgc cgcgcagttc gtgtgctggc ggcccgacaa
       61 gcggccgcag gagtgccgct acgatcagct cgaggtcacg ccggcttacg agctggagcg
      121 cgtcttcggc gcgcgcggca cccgcccggg tcgcggcgct cgcggtctcc cgacgtagcg
      181 gctagccgtc catttgcagc ctgctcaaga agaagcaaag gcccggatcg aacccgcaat
      241 ccagctcgcg gccaatttct gcctgccgga gcacgaaatc gctaagatct gattccgaag
      301 cgacagcggg gaatccgaga ggcttgaatc gacaccttgg aagttgacgt ctcggacgca
      361 tcgagggttg cattctgcgg tttccccgcg gatcgagagc gcttcgttca cttccgagga
      421 cgctctcctt ctcttgcgtc gacgtcgcat cttcagatga acctgaggcg cggcttcccg
      481 tcggaaaccg acgcggccgc tgcggcctga gatgagcatg gatcggagga actcccatcc
      541 gaaggacaac ctggcagaaa tcatcggaga cgaggctcgc ctcgaagcgc tgcggcgaac
      601 gtcactcctc gattccccgc ccgaggaggt gttcgaccgg ctgacgcgac tggcgacagc
      661 cgtcctgcac gtcccgctgg cgatggtttc gctcgtggac agcgatcggc agtttttcaa
      721 gagccagtgc gggctgtccg agccccttgc ctccttgcgg gagacccctc tcaccaactc
      781 gttctgcaaa catgccgtcg gatcgcgcga gcccctcatc gttgcggacg cccgtcggga
      841 tccggttttc gcgcagagtg ccgacagctc cgacgtcggg gtgatcgctt atgcgggagt
      901 cccgctgatc acatccgacg gctatgcact ggggacgttc tgcatcgtgg accaggagcc
      961 gcacgactgg acggacgagg agatcgggat cctccgggtc ctcgccacct ccacgatgtc
     1021 cgagatcgag ctgcggcgtc tcgtcggcga gctgcgagcc ttgaccacga acctgcaggg
     1081 ggtcgtggag gcccgcactt ccgagcttcg cgcttcggag gagcgccagc gcgtccttct
     1141 cgaggtcaac aatgcaatcg tcacctgcct cgaccgggaa tctctgttcc gcgcgaccac
     1201 ggccgcgctg gaaagcgtga tcccgttcga ccgcgcggcg ctccacatct acgatcccga
     1261 caagcaggtc ctccgagtgt tcggtgtgac cgggcccgtg cccctgtctc cgatctttcc
     1321 gatcggcacc gaattgcctc gacagcagag tcgcaatgga tgggtgctcg atcacggcgt
     1381 tcctctcatc acgttcgaca tgcggaatcc gcctccgttc ctcgagcacc ccgctctcgt
     1441 ccagggaggt ctccgctctg ccgtctcggt tccgatgacc gtcaaaggaa agatcatcgg
     1501 aaccctgcac gtcggaagcc gcgtgtgcgg ggcctacggg gccgacgagg cctcgctgct
     1561 cctcgcgatc gccgagcagg tgggactcgc gatcgagaac ctgctcgcct acgaggagat
     1621 cgccgcgctc aaagcacgcc tcgaagagga gaacgtctat ctccaggagg aggtacgcac
     1681 cgaggccgca ttcggcgacg tcatcggcga gtcgccgcag atactcgccg tcctcgcggg
     1741 cgtgcggaag gtcgcgaaga ccgactccac ggtgctggtg accggcgaga ccggcaccgg
     1801 caaggagctg atcgtccgcg cgatccacga tctctcccgg cggaaggaca agctcctcgt
     1861 gaaggtgaac tgcgccgcgc tcccggccgg cgtgatcgaa agcgagctct tcgggcacga
     1921 aaggggcgcg ttcaccggcg cactcacgcg gaaagtcggc cggttcgagc tcgcaaaccg
     1981 gggcacgctc ttcctggacg aggtcggcga ccttccgctg gagctgcagg cgaagctcct
     2041 gcgcgtgctt caggagggcg agttcgagcg cgtcggcggg acacagacgt tgaaggtgga
     2101 cgtgcggctg atcgcggcca cgaatcgcga cttggagcgc gccgtgagcg aggggcactt
     2161 ccgcgcggac ctctattacc gtttgaacgt cttccccatc gtcattccac cgttgcgaaa
     2221 gaggctgcag gacgtcccgc gccttgcccg gcatttcgcc atgttgtacg cgtcgaagat
     2281 ggggaagaac gtgggcccgt tgaacactga cgtgctggag cggctggccg cctacgcgtg
     2341 gcccgggaac
//
LOCUS       DASWAV010000174         1628 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U2665
            k141_90575, whole genome shotgun sequence.
ACCESSION   DASWAV010000174 DASWAV010000000
VERSION     DASWAV010000174.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960316
            Sequence Read Archive: SRR10260007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 1628)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1628)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:47:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,929
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1628
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_90575"
                     /isolate="SMAG_U2665"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.36444 N 132.31889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..190
                     /locus_tag="VF958_03410"
     CDS             <1..190
                     /locus_tag="VF958_03410"
                     /inference="COORDINATES: protein motif:HMM:NF019095.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="CHRD domain-containing protein"
                     /protein_id="HEX9148186.1"
                     /translation="AVELNPGFSGEVLDGSVSVESDLSARILANPSQYYVKLHSLKFP
                     GGAIRGQLHTCGPETKNP"
     gene            241..1530
                     /locus_tag="VF958_03415"
     CDS             241..1530
                     /locus_tag="VF958_03415"
                     /inference="COORDINATES: protein motif:HMM:NF014081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX9148187.1"
                     /translation="MLTVDASDTVIENGAVAVEGGAIAAVGSRRSIESRFRARRTIDA
                     RGRLVLPGFVNAHTHAPMTLLRGAKDDVDLMTWLTKYMFPVERRFVSPEFVRWGALLA
                     CWEMIASGTTTFADGYFYEEEVARAADEAGLRAVPGQGIFDVPTPDSKTSAEGLARAE
                     KFLSDWGGHPRITPSLCPHACYTVEPDTWRRTMNLAERHGAPVQTHLAESPAEIAMVR
                     ERFETTPVRHLSRVGVLNERLTAAHCVCVDDEEIELLARSGAGVVHCPESNMKLASGV
                     APVPKMLAAGVRLGLGTDGAASNNDLDMVGEMGSAARLQKVSTLDPTAASAHQVLRMA
                     TLGGARALHMEDRIGSLEPGKRADLIVLDLSTPAALPLFDPISYVVYSARSDAVETVI
                     VEGKVVMDRRRMRTLDTEKIRRRVERFGRKIRAALPD"
BASE COUNT          254 a          550 c          578 g          246 t
ORIGIN      
        1 ggcggtcgag ctcaacccgg gattttcggg cgaggtcctc gacggctcgg tttcggtcga
       61 gtccgacctc tcggcgcgca ttctcgcgaa cccgtcgcag tactacgtga agcttcacag
      121 cctgaagttt ccgggcggcg caatccgcgg gcagctgcac acgtgcggtc ccgaaacaaa
      181 gaacccctga gccgaccgga tcgggtcgcg cgcgccgacc tcgtcatcgc cggcggaacc
      241 gtgctcacgg tcgacgcgtc cgacacggtg atcgaaaacg gcgccgtcgc ggtcgaaggc
      301 ggagcgattg cggcggtcgg gtctcggcgc tcgatcgagt cccgcttccg cgcccggcgg
      361 acgatagacg cccgcggccg gctagtgctt cccgggttcg tcaacgcgca cactcacgcg
      421 cctatgacgc tgctccgtgg cgcgaaggac gacgtcgatc tcatgacgtg gctgacgaag
      481 tacatgttcc cggtcgagcg gcgattcgtc tcgccagagt tcgtccgctg gggcgcgctg
      541 ctcgcgtgct gggagatgat cgcctccggc acgacgacgt tcgccgacgg ctacttctac
      601 gaggaagagg tcgcgcgggc ggcggacgag gcggggcttc gcgccgtgcc ggggcaggga
      661 atcttcgacg ttccaacgcc ggactcgaag acgtcggcgg aaggcctggc gcgcgccgag
      721 aagttcctgt ccgactgggg cggccacccg aggatcacgc cttcgctctg cccccacgct
      781 tgctacacgg tcgagccgga cacctggcgg cggacgatga acctcgcgga gcgccacggg
      841 gcgcccgtcc agacgcatct cgccgagtcg cccgccgaga tcgcgatggt gcgcgagcgg
      901 tttgaaacga cccccgtccg ccacctttcc cgggtcgggg tgttgaacga gcggctcacc
      961 gcggcgcact gcgtgtgcgt ggacgacgaa gagatcgaac tgctggcgcg gagcggcgcc
     1021 ggcgtcgtgc actgccccga gagcaacatg aagctcgcga gcggcgtcgc gccggtgccg
     1081 aagatgctcg ccgcgggcgt gcggctgggg ctcgggaccg acggagccgc gagcaacaac
     1141 gacctcgaca tggtcggaga gatgggttca gccgcccggt tgcagaaggt ctccactctc
     1201 gatccgacgg cggcttcggc ccaccaggtc ctgcggatgg cgacgctcgg gggcgcgcgg
     1261 gcgctccaca tggaggaccg catcgggtcg ctcgagccgg gcaagcgcgc ggatctgatc
     1321 gtgctcgatc tgtcgacacc ggccgcgttg ccgctcttcg atccgatctc ctacgtcgtc
     1381 tactccgcgc ggtccgacgc cgtcgagacc gtcatcgtcg aggggaaggt cgtgatggat
     1441 cgtcggcgga tgcggacgct ggacacggag aagattcgcc gccgcgtcga gcgattcggg
     1501 cgaaagattc gcgcggcgct gccggattga ccccctcacc ctaaccctct ccccgcgggc
     1561 ggggagaggg aacagttgtc aggagcctcg cgccaagcac gaggagcccc agcgcggcat
     1621 acgccgcg
//
LOCUS       DASWAV010000175         6644 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U2665
            k141_329137, whole genome shotgun sequence.
ACCESSION   DASWAV010000175 DASWAV010000000
VERSION     DASWAV010000175.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960316
            Sequence Read Archive: SRR10260007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 6644)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6644)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:47:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,929
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6644
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_329137"
                     /isolate="SMAG_U2665"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.36444 N 132.31889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..629)
                     /locus_tag="VF958_03420"
     CDS             complement(<1..629)
                     /locus_tag="VF958_03420"
                     /inference="COORDINATES: protein motif:HMM:NF024882.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK 4TM domain-containing
                     protein"
                     /protein_id="HEX9148188.1"
                     /translation="MEGGIFKSRAVSEALGVLLLLAGVLALLSLYSYDPRDPNLFSLS
                     TGETESPANWIGGFGSSLSAALYQTLGFAAWTVPVLLAFWGVRRFASRPFENRGSKAL
                     GFTLLFLAVPALLSLAFGRRPISGEDAEAGGIVGRAVSEAARSKLGTTGATLLVVTMI
                     LIAVPLATQVSLADVFFGLRVKLVGLLSRFRLGWARRRDKRTKERLRRTV"
     gene            689..1132
                     /locus_tag="VF958_03425"
     CDS             689..1132
                     /locus_tag="VF958_03425"
                     /EC_number="1.20.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012873818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030612 - arsenate reductase (thioredoxin)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase ArsC"
                     /protein_id="HEX9148189.1"
                     /translation="MKKRVLFLCTGNAARSQMAEALARADYGDVLDPVSAGSRPAELV
                     HHLAIHAIEELGISMDGAHTKGADEFAGAPFDVVVTVCDSASQDCPTWPGAKRIEHWS
                     IEDPSWTDPEIRFARFRETRDELRRRIGDLVASLSTPSPSGRGTG"
     gene            complement(1151..2116)
                     /locus_tag="VF958_03430"
     CDS             complement(1151..2116)
                     /locus_tag="VF958_03430"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEX9148190.1"
                     /translation="MKRTMAGLVLSFATMALAQNHHQQAPTDPFRLQPLSGGVYALYG
                     RGGNVAFLVGPEAVLVVDSQFRDLAPGIVEKIKSISDKPIKYLVNTHHHPDHVGGNEV
                     FKQFAIIVAHDNVRKRMLASPAEILKDYPARADAARKSGNEEFAGRLEEMIAWAKRVR
                     VEEIPAPFVTFETDLQIYIGGETVHVWHTTPAHTDGDSVVYFEKAKVLHMGDLLFNKV
                     IPVIDVKGGGTPKGYVPALDSILARLEPGVTIIPGHGEVTDAAGVRAFRQYIVDLTEL
                     AKSAKTAGKSREAFVSEAELPAYKDWNGYPQRFKENAGAAFDEVR"
     gene            2344..3372
                     /locus_tag="VF958_03435"
     CDS             2344..3372
                     /locus_tag="VF958_03435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018206905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HEX9148191.1"
                     /translation="MRVGVLTGGGDAPGLNAVIRAVVRKGEKAYADELTGFADGWRGL
                     IEGKAVALNSEGTRGLLPRGGTILGTSRTNPFKVDGGPEKVEGTIARLRIEALIAIGG
                     EDTLGVALKFFERGVPVVGVPKTIDNDLSGTEVTFGFDTAVQIAVDAIDRLHTTAESH
                     NRVLICEVMGRHAGWIATLAGIAGGAAYILVPEREFDIARVCDSLVERHEKGRYASII
                     VVAEGAQPRQGSMVVQSGEVDEFGHVRLGGIGEVLAREIRQRTGFDTRVTVLGHVQRG
                     GTPTAFDRVLATRFGIAAIDAVHDGAFGQMVALSDAKIVRVPIADAVGRLKTVDPDLL
                     EVAEIFFG"
     gene            complement(3373..6474)
                     /locus_tag="VF958_03440"
     CDS             complement(3373..6474)
                     /locus_tag="VF958_03440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020738400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HEX9148192.1"
                     /translation="MKLTDACIEKPVFAWMLMAATVVFGGVAASRIGISQFPDVDFPT
                     ISVGITWEGASPEVVENDAIEILEESLVQVEGVKSITSSSRQGGGSITVELDLSRDVD
                     LALQDVQTKVSQSQQRLPRDMDPPVISKTNPEDQPIMWIGLSGPFPQRVLADYARYRV
                     KEKLQTVPGVGEITMGGYLERNVRIWVDSSRLDEKGLTVADVNDALRREHVELPAGRL
                     EGEGREVNVRVLGEALDLATLRRIVVREVAGSPVYLEDVALVEDGFEDVRRLSRVDGQ
                     PAQGIGVRKQRGANAVAVARGIRTTLAEIRKTLPPGMLLAINFDSTKFIEDSVHEIQL
                     ELALSVLLTAFVCWLFLGSISSTLNVVLAIPMSLLGTVAAIYFLGFTFNTFTLLGLAL
                     AVGIVVDDAIMVLENIYRHGEMGKGRVQAAREGTKEITFAALAATLAVVAIFSPVVFM
                     KGVIGRFFLQFGITLCIAVLLSYVEAMTLAPARCAQFLQTSREGRSRIGLVVDQLFGR
                     LERLYGRALRGGLKRPALVLSAGAALFVISFFVLKGLPKEFVPSQDQSRFIVRLQTAV
                     GSDLMETDKIFRQAEAVLAKRPEIIRNYTVIGGGGGGSVSGGFMFITLVPPRERKLSQ
                     AEFSAVLRKQLNGIPGLKAVIQDLSQSGFTAQRGFPVEFSVRGSDWDGLVGASDSISS
                     KLAESGVVVDLDTDYRLGMPELRILPDRARAADLGVPIEDVATTLNSLVGGIRVGKYS
                     SGGRRLDVRLRLLASQRTRPEDLARLRVRARSGELVPLSLLVTTEEKPALQAITRRDR
                     ERAITVFGNIAPGHSQDEALARVEELAKHMPVGYRAVLGGASVAFRESMSSLLFALAL
                     GIIVAYMVLASQFNSFLHPVTVLTILPLSIAGAAFALWIAGKSLNIFSMIGLLLLMGI
                     VKKNSIILVDYANQARAEGLNARDAMLRAGPIRLRPILMTSVATLMAALPPALGLGSG
                     SEIRTPMAIAVIGGLIVSTALSLLVVPAFYVTADRVASKVRARFPARRPRRTAEAEHG
                     AGS"
BASE COUNT         1146 a         2176 c         2274 g         1048 t
ORIGIN      
        1 accgtgcggc gcagcctctc cttcgtgcgc ttgtcccgcc ggcgggccca cccgaggcgg
       61 aaacgcgaca acagcccgac gagcttgacg cgcagcccga agaaaacgtc cgcgagcgag
      121 acctgtgtcg cgagaggaac ggctatgagg atcatcgtca cgacgagaag tgtcgcgccc
      181 gtcgtgccga gcttcgagcg cgccgcttcc gacaccgccc ggccgacgat cccgccggcc
      241 tccgcgtcct cgccggagat cggccgccgc ccgaacgcga gcgacagcag cgcggggacc
      301 gcgagaaaga gaagcgtgaa ccccagcgcc ttcgagcccc ggttctcgaa cggacgcgac
      361 gcgaacctcc ggactcccca gaaggcgagg aggaccggca cggtccaggc ggcgaagccg
      421 agcgtctggt agagcgccgc cgagagcgag gaaccgaatc cgccgatcca gttcgcgggc
      481 gactcggtct cgcccgtcga gagcgagaac agattggggt cgcgcgggtc gtaggagtac
      541 agcgagagaa gagcgagcac tccggcgagc agcagtagga cgcccagggc ctcgctgacg
      601 gcccggctct tgaagattcc tccttccaag tcgcttcatt atgaggctca aaggccgcgg
      661 agaacagggt gacttatgct cgaaagcgat gaagaagcgg gttctcttcc tctgcacggg
      721 gaacgcagcg cggagccaga tggccgaggc gctcgcgcgt gcggactacg gggacgttct
      781 cgatccggtc tccgcggggt cccggccagc ggagctcgtt caccacctgg cgattcacgc
      841 gatcgaggag ctcggcatct cgatggacgg cgcccacacg aaaggcgccg acgagttcgc
      901 cggcgcaccc ttcgacgtgg tcgtcaccgt gtgcgattcg gcgtcgcagg actgccccac
      961 ctggcccgga gcgaaacgca tcgaacactg gtcgatcgag gatccctcgt ggacggaccc
     1021 cgagatccgt ttcgcgcgct tccgcgagac ccgcgacgag ctgcgacggc ggatcggcga
     1081 cctcgtcgcg tcgttgtcga ctccctctcc ctccgggaga gggacggggt gagagggctg
     1141 accgagcggg ttacctgacc tcgtcgaacg ccgcgccggc gttctccttg aagcgctgcg
     1201 gatatccgtt ccagtccttg taggcgggca gttccgcctc cgagacgaac gcctcccggc
     1261 tcttgcccgc cgtcttcgcg ctcttcgcga gctccgtcag atcgacgatg tactgtcgaa
     1321 acgcccgtac tcctgcagca tccgtgacct cgccgtgccc cggaatgatg gtcacaccgg
     1381 gctcgaggcg ggcgaggatc gagtcgagcg ccggcacgta gcctttcggc gtgccccctc
     1441 ccttgacgtc gatcacgggg atgaccttgt tgaacagcag gtctcccatg tgaaggactt
     1501 tcgccttctc gaagtacacg acgctgtctc cgtcggtgtg tgcgggcgtc gtgtgccaga
     1561 cgtgaacggt ctcgccgccg atgtagatct gcaggtccgt ttcgaaggtg acgaaaggcg
     1621 cgggaatctc ctcgacccga accctctttg cccaggcgat catttcctcg agccgtccgg
     1681 cgaactcctc gttgccgctc ttcctcgcgg catcggcgcg agccggataa tccttgagga
     1741 tctcggcggg cgaggcgagc atccgttttc ggacattgtc gtgcgcgacg atgatcgcga
     1801 actgcttgaa cacttcgttt ccgccgacgt ggtcggggtg gtggtgcgtg ttgaccaggt
     1861 acttgatcgg cttgtccgag atcgacttga tcttttcgac gatgccgggg gcgaggtcgc
     1921 ggaactgcga gtcgacgacg agcaccgcct ccgggccgac gagaaaagcg acgttgccgc
     1981 cccggccgta gagggcgtag accccgcccg acagcggttg aaggcgaaac gggtcggtgg
     2041 gggcctgctg gtgatggttc tgtgcgagcg ccatcgtcgc gaacgaaagg acgagtccgg
     2101 ccatcgttct tttcatccgc cggactctag cggatcacga atggcgcaag aaagtggtcg
     2161 ggcgtggccc tcggcggggg ggcgaccgaa gcgctcttcg aggcgccgtg ccgccgggtc
     2221 gccttgatcg cagaggacgg cgacgggaac gacgggcccg aaaccgtcca gttttcgacg
     2281 cgcctcccgg cagggccacc tcttctcgga cggctcgggc taaagtcgtg ccgggaggcg
     2341 acgatgcgcg tcggtgttct gacggggggc ggggatgcgc ccgggctgaa cgcggtgatc
     2401 cgggcggtgg ttcgcaaggg tgagaaggcg tacgcggacg agcttaccgg gttcgcggac
     2461 gggtggagag gcctgatcga ggggaaggcc gtcgctttga actcggaagg cacacgcggc
     2521 ctcctgccgc gcggagggac gatcctcgga acgtctcgca cgaatccgtt caaggtcgac
     2581 ggcgggccgg aaaaggtcga ggggacgatc gcccgtttgc ggatcgaggc cctgatcgcg
     2641 atcggcggcg aggacacgct gggcgtcgcg ctgaagttct tcgaacgggg ggtccccgtc
     2701 gtcggcgttc ccaagacgat cgataacgat ctgtcgggta cggaggtgac gttcggcttc
     2761 gacaccgccg tccagatcgc ggtcgacgcg atcgatcggc tgcacacgac ggccgagtcg
     2821 cacaaccggg tgctgatctg cgaggtcatg ggccgccacg ccggatggat cgcgaccctg
     2881 gctgggatcg cgggcggcgc cgcctacatc ctcgtgccgg agcgcgagtt cgacatcgcc
     2941 cgtgtctgcg acagcctggt cgagagacac gaaaagggcc gctacgcctc gatcatcgtg
     3001 gtcgcggagg gcgcccagcc caggcagggc tcgatggtcg tgcagtcggg cgaggtcgac
     3061 gagttcgggc acgtgcggtt gggaggaatc ggagaggtcc tcgcacggga gatccggcag
     3121 cggacggggt tcgacacgcg cgtgacggtg ctcggccacg tgcagcgcgg cggcacgccc
     3181 acggcgttcg accgcgttct cgcgacccga ttcgggatcg cggcgatcga cgccgtccac
     3241 gacggcgctt tcgggcagat ggtcgccctc tccgacgcaa agatcgtccg cgtccccatc
     3301 gcggacgcgg tcgggagact caagaccgtc gaccccgacc tgctcgaagt ggcggaaatt
     3361 ttcttcggct gatcaggagc cggcgccgtg ctccgcttcg gccgtgcggc ggggccgtcg
     3421 ggcgggaaag cgggcacgca ccttcgacgc aacccggtcg gcggtgacgt aaaacgccgg
     3481 aacgaccagg agcgacagcg cagtcgagac gatgagtccg ccgatgaccg cgatggccat
     3541 gggcgtgcgg atctcggagc ccgagcccag gccgagcgcc ggcggaagcg ctgccatgag
     3601 cgtcgcgacc gacgtcatga gaatcggccg caggcggatc ggaccggcgc ggagcatcgc
     3661 gtcccgggcg ttcagaccct cggcgcgcgc ctggttggcg tagtccacca ggatgatcga
     3721 gttcttcttc acgattccca taagcagaag gagaccgatc atgctgaaga tgttcaggct
     3781 cttccccgcg atccacaacg cgaacgccgc ccctgcgatc gagagcggca ggatcgtcag
     3841 gaccgtcacc gggtgcagga acgaattgaa ctgcgaggca aggaccatgt aagcgacgat
     3901 gatcccgaga gcgagcgcga agaggaggct cgacatggac tcgcgaaacg cgacgctcgc
     3961 tccgccgagc accgcgcggt accccaccgg catgtgcttc gcgagctcct cgactctggc
     4021 gagggcctcg tcctgcgagt ggccgggggc gatgtttccg aaaacggtga tcgcgcgctc
     4081 gcggtcgcgc cgcgtgatcg cctggagcgc cggcttttcc tccgtcgtca cgagaagcga
     4141 cagcgggacg agttcgcccg accgggcgcg cacccggagg cgcgcgaggt cttcgggacg
     4201 cgtgcgctgc gatgcgagca ggcggaggcg aacgtcgagt cgccgtccac ccgagctgta
     4261 cttgccgacc cggatgccgc cgacgagcga gttcagagtg gtcgcgacgt cctcgatcgg
     4321 gacgccgagg tcggcggcgc gcgcgcggtc cggaaggatg cgcaactcgg gcatcccgag
     4381 ccggtagtcc gtgtcgaggt cgacgacgac gccgctctcc gccagcttgc tggagatcga
     4441 atcactcgcc ccgaccagtc cgtcccagtc ggagccgcgc accgagaact cgaccgggaa
     4501 gccgcgctgc gccgtgaagc ccgactgcga gaggtcctgg atcacggcct tcagccccgg
     4561 gattccgttc agctgcttgc gcaggaccgc cgagaactcg gcctgcgaga gcttgcgctc
     4621 ccgaggcggg acgagcgtga tgaacatgaa gcctccgctc accgacccgc ctccgccgcc
     4681 cccgatgacc gtgtagttgc gaatgatctc cggacgtttc gcgagaactg cctcggcctg
     4741 ccggaagatc ttgtcggtct ccatcaagtc cgatccgacc gcggtctgga gccggacgat
     4801 gaagcgactc tggtcctgcg atggaacgaa ttctttcggc aaccccttca gcacgaagaa
     4861 agaaatcacg aagagtgcgg ctccggcgga gaggacgagc gccgggcgct tcagcccgcc
     4921 gcgcagcgcg cggccgtaga gccgctcgag acgcccgaag agctggtcga cgacgaggcc
     4981 gatccggctg cggccctcgc gcgacgtctg aaggaactgc gcgcaccgcg ccggcgcgag
     5041 cgtcatcgcc tcgacgtacg agagaagaac cgcgatacac agcgtgattc cgaactggag
     5101 gaagaacctc ccgatcaccc ctttcatgaa gacgaccggg ctgaagatgg ccaccaccgc
     5161 gagcgtggct gcgagagcgg cgaacgtgat ctccttcgtc ccctctcgcg ccgcctgaac
     5221 gcgtcccttc cccatctcgc cgtggcggta gatgttttcg agcaccatga tcgcgtcgtc
     5281 gaccacgatg ccgacggcga gcgcgagccc gagcagagtg aaggtattga acgtgaatcc
     5341 gaggaaatag atcgcggcca ccgtgccgag cagcgacatc gggatcgcga ggacgacgtt
     5401 caacgtactc gaaatcgatc ccaggaagag ccagcagacg aacgcggtca ggaggacgga
     5461 gagggcgagc tcgagctgga tctcgtgaac ggagtcctcg atgaacttcg tcgagtcgaa
     5521 gttgatcgcg agcagcattc ccggcgggag cgtcttccgg atctcggcaa gcgtcgtccg
     5581 gattccgcgc gcgaccgcaa cggcgttggc gccgcgctgt ttgcgtaccc cgatgccctg
     5641 cgccggctgg ccgtcgaccc tcgaaagccg gcggacgtcc tcgaagccgt cctcgacgag
     5701 agccacgtcc tcgaggtaga cgggactccc ggccacctcg cggacgacga tccttcggag
     5761 agtggcgagg tcgagcgcct ctccgaggac acggacgttg acctcgcgac cctccccctc
     5821 gaggcggccg gccggcagct cgacgtgctc gcggcggagg gcgtcgttga cgtcagcgac
     5881 cgtcagccct ttctcgtcga gacgcgagga gtccacccag atgcggacgt ttcgctcgag
     5941 gtacccgccc atcgtgatct cgccgacgcc cgggaccgtc tggagttttt ccttcacgcg
     6001 gtaacgcgcg tagtcggcga gcactcgctg cgggaacggt ccggagaggc cgatccacat
     6061 gatcggctgg tcttccggat tggtcttcga gatcaccgga ggatccatgt cgcgcgggag
     6121 ccgctgctgc gactgcgaga ccttcgtctg cacgtcctgg agtgcgaggt ccacgtcacg
     6181 ggagaggtcg agctcgacgg tgattgagcc gccgccctgc cgcgacgacg acgtgatcga
     6241 cttgacgccc tcgacctgca ccagcgattc ctcgaggatc tcgatcgcgt cgttttcgac
     6301 gacttcgggc gacgctccct cccacgtgat cccgactgaa atcgtcggaa aatcgacgtc
     6361 ggggaactgg ctgatcccga tccgcgacgc cgcgaccccg ccgaacacga ccgttgcggc
     6421 catcagcatc caggcaaaga cgggtttctc gatgcaggca tcggtcagct tcatggcgtt
     6481 gctccggagg tgacggatga aagagctccc gctccctcgc cgctgcgggt gactttgact
     6541 ttcgcgccgt cccggagcgc ctcgcctccg cgcacgacga gctgctctcc cggcttgacc
     6601 cccgagcgca cttcgacgcg tccgtccgcg gtgcgcaacc cgag
//
LOCUS       DASWAV010000176         1596 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U2665
            k141_130497, whole genome shotgun sequence.
ACCESSION   DASWAV010000176 DASWAV010000000
VERSION     DASWAV010000176.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960316
            Sequence Read Archive: SRR10260007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 1596)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1596)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:47:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,929
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1596
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_130497"
                     /isolate="SMAG_U2665"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.36444 N 132.31889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            49..>1596
                     /locus_tag="VF958_03445"
     CDS             49..>1596
                     /locus_tag="VF958_03445"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX9148193.1"
                     /translation="MKILHSVGDQLAVALERAHLRGHLETLAEERTAALKAEIVKRKG
                     TEKRLRESEERYRLMFDKNPHPTWVYDAETLAFLAVNEAAIHHYGYSRGEFLGMTIKD
                     IRPAEEVPALLRVLSENPHTPLTRVSVNVKHRKKDGTVIDVESASSAITFSGRAAALV
                     LAMDVTERLRLEGERHRMEGQLEQGRRVESLGRVAATIAHEFNNVLMGIQPFAEVINR
                     NTSADAKIRKAAGQIIHSVARGKRVTEDILRFTRASEPVLRPVDLGEWIEQLVPELRG
                     MIGDRIEIRVDIPERPVVARCDPAQLQQVVANLAVNARDAIPGSGTIFITIASGRDDT
                     RFRSGTFPPGMVLLALRDTGSGMPREILDHIFEPLFTTKRTGTGLGLAVAQRVIARHG
                     GMIDVESEPGKGTTVSILLPAATLESPKAAPAAMVRHPRRVLLVEDEPEVAAGIAALL
                     ESDGMEVRSVACGRDAAPAARSFEPDAVILDVALPDMSGIDVFAELRLSWPKLPVLFS
                     SGHGDQAA"
BASE COUNT          313 a          484 c          521 g          278 t
ORIGIN      
        1 gtgatgaatc tgatcgggcc ggaacagagt ccgttcgcag aggaagagtt gaaaatcctg
       61 catagcgtcg gcgaccagtt ggccgttgcg ctggagcggg cacacttgcg cggacatctg
      121 gagacgttgg cggaggaaag aaccgccgct ctgaaagccg agatcgtgaa gagaaagggc
      181 acggagaagc gccttcgtga gagcgaggaa cgctatcgac tgatgttcga caagaatccc
      241 cacccgacat gggtgtatga cgcagaaacg cttgcgttcc tcgccgtcaa cgaggccgcg
      301 attcaccatt atgggtattc gcggggcgag tttctgggca tgaccatcaa agacatccgt
      361 cctgccgagg aggtgcccgc actgctgcgc gtgctctccg agaatcccca tactccactc
      421 acacgagtgt ccgtgaacgt caagcaccga aagaaagacg gcacggtgat cgatgtggag
      481 agcgccagca gcgcgatcac tttctccggt cgcgcggccg ctctcgtact ggcgatggat
      541 gtgacggaaa gactccgtct cgagggcgag cgtcaccgga tggaggggca gctggaacag
      601 ggacgacgtg tcgaatcgct cggcagagtt gccgccacga tcgcccacga gttcaacaat
      661 gtactgatgg gtatccagcc ctttgccgaa gtcatcaacc gcaacaccag tgcggacgcg
      721 aagattcgga aggcggccgg gcaaatcatc cactcggtgg cccgcggaaa acgcgtgacg
      781 gaggacattc tccgcttcac acgtgcctcg gagcctgtcc tccggccggt tgatctcggc
      841 gagtggatcg agcagctcgt accagagctg cggggcatga tcggtgaccg gatcgagatt
      901 agggtggaca tccccgagcg gccagtggtt gcacgttgtg atccggcgca actccagcag
      961 gtcgtcgcca atctggccgt caacgcgcgt gacgcgatcc ccgggtcagg gacgatcttc
     1021 atcacgatcg cgtccggtcg ggacgacacg cgcttccgat ccggcacgtt ccctcccggg
     1081 atggttctcc tcgccctccg tgacaccggt tcagggatgc cgcgcgagat cctcgaccat
     1141 attttcgagc ccttgttcac cacaaagcga accgggaccg ggctcgggct cgcagtggcg
     1201 caacgcgtga tcgcgcgtca cggagggatg atcgacgtcg agagtgagcc cggcaagggg
     1261 acgaccgtgt ccatcctctt gcccgccgct acgctcgaga gcccgaaagc tgcgccggcg
     1321 gcgatggtac gtcacccgcg cagagttctc ctggtcgaag acgaaccgga ggtagcggct
     1381 gggatcgcgg cactgctcga gagcgatgga atggaggttc ggagcgtcgc ctgtggacgc
     1441 gacgctgcgc ccgctgcgcg ctctttcgaa ccggatgccg tcatcctcga tgtcgccctg
     1501 ccggacatga gcgggatcga cgttttcgcc gagctgcggc tgtcatggcc gaagcttcca
     1561 gtcctcttct cgagcggtca cggcgaccag gccgcg
//
LOCUS       DASWAV010000177         5505 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U2665
            k141_410694, whole genome shotgun sequence.
ACCESSION   DASWAV010000177 DASWAV010000000
VERSION     DASWAV010000177.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960316
            Sequence Read Archive: SRR10260007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 5505)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5505)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:47:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,929
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5505
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_410694"
                     /isolate="SMAG_U2665"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.36444 N 132.31889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..615)
                     /gene="ispE"
                     /locus_tag="VF958_03450"
     CDS             complement(<1..615)
                     /gene="ispE"
                     /locus_tag="VF958_03450"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: protein motif:HMM:TIGR00154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050515 - 4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HEX9148194.1"
                     /translation="MSAEPVLSAHAPAKVNRELRVGELRPDGYHEIRSRLVSIDLVDT
                     IEVAAGGHGIELSCDGIPVPCDDSNLVAVAARALAERIGRPPDVCIHLTKRIPVGAGL
                     GGGSSDAARTLALLDRLWRARLGLAELSQVAAGIGSDVPFFLVGGEADVSGRGEIVSP
                     APDSPGVDLLLVIPPFAMSTAEVYAAFRRGASGGDRRVPASLDVQ"
     gene            complement(626..1297)
                     /locus_tag="VF958_03455"
     CDS             complement(626..1297)
                     /locus_tag="VF958_03455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9148195.1"
                     /translation="MRARASRRRGFRALLLAASLGASAYGCASRPAAFGPAGAEDSRR
                     TLDAWGAALERADSLGPARILYEAKMSEGLVRIPGTLSVETRSDRLEATLVGPFGSPV
                     ARYSSGVLEANGAKPIPLDAEELRAVLAGVWRSPPRVEGAREGESLIRFTGRDRVEAV
                     LDVASTRVETLKIERPEAELVATYSGRLDPWPEKITIEDRRSGKTLRLTMVAREPIDA
                     PAVQP"
     gene            complement(1281..3170)
                     /locus_tag="VF958_03460"
     CDS             complement(1281..3170)
                     /locus_tag="VF958_03460"
                     /inference="COORDINATES: protein motif:HMM:NF025910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX9148196.1"
                     /translation="MKARLAAIMAAVLLCGTLAAQDLRETRKLFLRARSAIAEGSYRE
                     ALDLYRKVIELVPQDAVVRFEYAQLLRDLNVSDEAMKQAQEVVRLDPQLVEGHRLLGA
                     LQLAASERDPSQLTLAIGELQAAWQLTPGDPAIAAPLARALLAKGKAAEAAKILEGAG
                     EAAAQPAIARIAAEAKAKSGRTKEAQAIYAALLEENPEDRESAAALIDLYEDDDQLEK
                     ALDLLQKLEKLDADNPAIPERITLDLARAGRFAEAEKRARDLAAKRPENRAIRRLLSQ
                     VLFEKGSSPEGEKILRMLVAEDPDDEQTRRTLTGELMRERRFSEAKTILEDGVRRATD
                     DPKKIPAREAATAELGYLAVLQKDYARAQQLLTPLAIKQGTVNPRAVRILATAAREKE
                     DFAAGLEVARAAAAAEPDKPEWKALVAEFQYRAGDRKKAEEALAKLATSPDIEEVLVA
                     GDVYARVKDFASAARVAREASSRFPDSSEALFRLGAALERTGATGEAEKTFEKILQGR
                     PNDSATQNYLGYMWADRGIRLEEARALLEKAVAREPRNGAYLDSLGWVYFRLGRLEKA
                     QSLLAEAKQREPDDPTIEEHLGDLSERQGDISKAIAHWERALELKHDEPDKVRQKLAR
                     FARKS"
     gene            3343..3612
                     /locus_tag="VF958_03465"
     CDS             3343..3612
                     /locus_tag="VF958_03465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020719403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HEX9148197.1"
                     /translation="MIKADIVDLVVDLAKIPRAKAATAVETVIESMRSALGAGDRIEL
                     RGFGVFQVKRRKRGIGRNPKTGVEVTIPPGNTIRFKPGKDLRDLP"
     gene            3639..4562
                     /locus_tag="VF958_03470"
     CDS             3639..4562
                     /locus_tag="VF958_03470"
                     /inference="COORDINATES: protein motif:HMM:NF014244.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HEX9148198.1"
                     /translation="MESEKDSPPPDPFAAPPRWERRPRWKIPEHPLFHLGLLAATFVT
                     TTVFGGAVFSDGGGPLRGSRFSDGFAFSIPLLLILGIHELGHYAVCRRHGVAATLPYF
                     LPAPIPNLIGTFGALIRIKEPIRDKRALIEIGAAGPVAGFLTAIPFLVYGVTRTTPIA
                     QQTMTPGTVVFEYPILVRFAQDLMGTGRFTSATVHEDPTFMAAWFGLLVTALNLLPIG
                     QLDGGHVVRAALGRRQPIFSYAVLVLAALSATRGPIWAFFCFFTALFLGVRHPPVEND
                     DEPLPFNHQMLALFCLAVFLLCFTLVPMKVV"
     gene            complement(4700..5137)
                     /gene="dtd"
                     /locus_tag="VF958_03475"
     CDS             complement(4700..5137)
                     /gene="dtd"
                     /locus_tag="VF958_03475"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011867337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacyl-tRNA deacylase"
                     /protein_id="HEX9148199.1"
                     /translation="MKLVIQRVSEAEVHVGDETVGRIGRGLLVLVGAEKGDDDAAADE
                     AARKVAGLRVFNDEGGKMNLDLAAIGGSVLVVSQFTLGADLSRGRRPGFERALAGEEA
                     RPLYERFVEALARRGVSVETGRFGSMMQVSLVNDGPATFILQV"
     gene            complement(5134..>5505)
                     /locus_tag="VF958_03480"
     CDS             complement(5134..>5505)
                     /locus_tag="VF958_03480"
                     /inference="COORDINATES: protein motif:HMM:NF026018.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis C-terminal
                     domain-containing protein"
                     /protein_id="HEX9148200.1"
                     /translation="LLLRPWGVGSVALATSCGAIANFVLLLASLRRREGRLGLKEVAA
                     SLLRVLVAAAVMALVAGWAAAWMLAAHPPRTAGTRLLFLVAATFAGMIVYLAVALALR
                     SPEPREFVRLLTQRRGQSKSS"
BASE COUNT          812 a         1931 c         1854 g          908 t
ORIGIN      
        1 ctggacgtcc agggatgccg ggacgcggcg gtcgcccccc gaggcgcccc gccggaacgc
       61 cgcgtagacc tcggcggtgg acatcgcaaa cggagggatg acgagcagca gatcgacccc
      121 cggcgagtct ggcgccggtg agacgatctc cccgcgaccg gagacatccg cctccccccc
      181 gacgaggaag aacggcacgt cgctgccgat cccggccgca acctgcgaca gctccgccag
      241 tccgagtcgc gccctccaga ggcggtcgag gagcgcgagc gtcctcgcgg cgtcagagct
      301 tccgcctccg agtccggcgc cgacggggat cctcttcgtc aggtggatgc agacgtcggg
      361 cgggcgcccg atccgctcgg cgagcgcgcg ggctgcgacg gcgacgaggt tggagtcgtc
      421 gcacggcacg gggatcccgt cgcacgaaag ctcgatgcca tgtcctccgg cggcgacctc
      481 gatcgtgtcc acgagatcga tcgagacgag tcgcgaccgg atctcgtggt aaccgtcggg
      541 acgcagctca ccaacacgca gctcgcggtt gaccttcgcc ggcgcgtgcg cggagaggac
      601 gggttcggcg ctcaaggctc gagggtcagg gctggacggc gggcgcgtcg atcggctctc
      661 tcgcgaccat cgtcagccgg agcgtcttcc ccgagcgccg gtcttcgatc gtgatctttt
      721 cgggccaggg atcgaggcgt ccggagtacg ttgccacgag ttccgcctcg ggccgctcga
      781 tcttgagggt ctcgacccgc gtcgacgcga cgtcgagcac ggcttcgacg cggtcgcggc
      841 cggtgaaacg gatgaggctt tccccttcgc gcgcgccttc gacccgtgga gggcttctcc
      901 agactccggc gagcaccgcg cgcaactctt ccgcgtcgag agggatcggc ttcgccccgt
      961 tggcctcgag aacgcccgag gagtagcgcg cgaccggcga cccgaatggg ccgacgagcg
     1021 tcgcttcgag gcgatccgat cgcgtttcta ccgacaacgt cccggggatc ctcacgagcc
     1081 cctcggacat cttcgcttcg tacaggatcc gggcaggacc gagggagtcg gcgcgctcga
     1141 gcgcggcacc ccaggcgtcg agcgtccggc gcgagtcctc cgctccggcg ggcccgaacg
     1201 cggccggccg cgaggcacag ccgtatgccg aggcgccgag ggacgcggcg agcaggagcg
     1261 cccggaagcc ccggcgccga ctagctcttg cgcgcaaacc gggcgagctt ctgtcgaacc
     1321 ttgtccggtt cgtcgtgctt caactccagc gcccgctccc agtgagcgat cgctttcgag
     1381 atgtcgccct gccgctccga gaggtcgccc aggtgctcct cgattgtggg gtcatcgggc
     1441 tcccgctgtt tcgcctcggc cagcaagctc tgtgctttct cgagacggcc gagccggaaa
     1501 tagacccacc cgagcgagtc gagatacgcc ccgttgcgcg gctcgcgggc gaccgccttc
     1561 tccaggagcg cccgggcctc ctcgagacgg atcccccggt cggcccacat gtagccgaga
     1621 tagttctgcg tcgccgagtc gttgggccgt ccctgcagga tcttctcgaa cgtcttctcc
     1681 gcctcgcccg tggccccggt tcgctccagc gcggcgccga ggcggaagag tgcctcggag
     1741 ctgtcgggga agcgggacga cgcctcacga gccacgcggg cggccgaggc gaaatccttc
     1801 acgcgggcgt agacgtcgcc ggccacgagc acttcttcga tgtccggaga agtcgcgagc
     1861 ttcgcgagcg cctcctccgc tttcttccgg tcccctgcgc gatactggaa ttcggcgacg
     1921 agggccttcc actcgggctt gtcgggctcc gccgcggcag ccgcgcgggc gacctcgagt
     1981 ccggcggcga aatcctcttt ctcgcgagcg gccgtcgcca gaatccgcac cgcgcgcggg
     2041 ttcaccgtcc cctgcttgat cgcgagcggc gtcaggagct gctgcgcgcg agcgtaatcc
     2101 ttctgcagga cggccaggta tccgagttcc gcggtcgccg cctcgcgcgc ggggatcttc
     2161 ttcgggtcgt cggtcgcgcg gcggactccg tcctcgagga tcgtcttcgc ctcggagaac
     2221 cgccgctcgc gcatgagctc tccggtcagc gtgcgacggg tctgttcgtc gtcgggatcc
     2281 tccgcgacga gcatgcggag gatcttctcc ccctcgggcg agcttccttt ctcgaagaga
     2341 acctgcgaga ggaggcggcg gatcgcgcga ttctcggggc gtttcgccgc aaggtcgcgg
     2401 gcgcgcttct cggcttcggc gaaccggccg gcccgcgcca gatcgagagt gatgcgctcg
     2461 gggatcgccg ggttgtccgc gtcgagcttt tcgagcttct gcagaaggtc gagcgctttc
     2521 tcgagctgat cgtcgtcctc gtagaggtcg atcagggcgg cggcgctctc ccggtcttcc
     2581 gggttctctt cgagaagcgc ggcgtagatc gcctgcgcct ccttcgtccg gccgcttttc
     2641 gcctttgcct cggcggcgat gcgggcgatc gccggctgcg cagcagcctc ccccgccccc
     2701 tcgaggatct ttgccgcctc ggcggccttc cccttggcga gaagcgcgcg ggcgagcggc
     2761 gccgcgattg ccggatcgcc cggcgtgagc tgccacgcgg cctggagctc tccgatggcc
     2821 agcgtgagct gcgaggggtc tctctcggag gcggcgagct ggagcgcccc cagcagccgg
     2881 tggccctcca cgagctgcgg gtccaggcgg acgacctcct gagcctgctt catggcctcg
     2941 tccgaaacgt tcaggtcgcg cagaagctgg gcgtactcga atcggaccac ggcgtcctgc
     3001 ggcacgagct cgatgacctt cctgtagagg tcgagggcct cccggtagct gccctcggcg
     3061 atcgccgaac gcgcccggag gaagagcttt cgggtctcgc gcagatcctg ggcggcgagg
     3121 gtcccgcaga gaaggacggc cgccatgatc gcagcgaggc gcgctttcat gatcgaccaa
     3181 acgcggaaag cgcggtgttc atgcccgcgg cgaatgtagc acgcgcgcgc ggcgtcaccg
     3241 gcgcgagtcc aaagtcccgg gtccaaagtc cagagccgcg ggaggagccc agaagttgct
     3301 ctgcctcaga gatataatgc ccggcaccga tccggagggg agatgatcaa agccgacatc
     3361 gtcgacctcg tcgtcgacct cgccaagatc ccgcgagcca aagcagcgac cgcagtggag
     3421 accgtgatcg agtcgatgcg ctccgcgctc ggcgccgggg accgcatcga gctccgcggt
     3481 ttcggtgtct ttcaggtgaa gcgccggaag cgcggaatcg ggagaaaccc caagacaggc
     3541 gtcgaggtca cgattccccc gggcaacacg atccgcttca agcccggaaa ggatctccgc
     3601 gaccttccgt agtttccacc gcctgatata agcctcgcgt ggaatcggaa aaggattccc
     3661 cgccgccgga cccgttcgcc gcgccgccgc gctgggagag gcgcccgcgc tggaagatcc
     3721 cggagcaccc cctctttcac ctcggtcttc tggccgcgac gttcgtcacg acgaccgtct
     3781 tcggaggcgc cgttttctcg gacggaggag gtccgcttcg cggcagccgt ttttccgacg
     3841 gtttcgcgtt ttcgattccg ctgctcctga tcctcgggat ccacgagctc ggtcactacg
     3901 cggtctgccg gcgtcacggc gtcgcggcga ctctccccta ttttcttcct gctccgattc
     3961 cgaacctcat cgggactttc ggcgctctca tccgaatcaa ggagccgatc cgcgacaagc
     4021 gcgcgttgat cgagatcggc gcggccggcc ccgtcgcggg gttcctgacg gcgattccgt
     4081 ttctcgtcta cggggtgacg cgcacgacgc cgatcgcgca gcagacgatg actccgggca
     4141 cggtggtctt cgaatacccg atcctcgtgc gcttcgccca ggacctgatg ggaacgggac
     4201 gcttcacgag cgcgacggtg cacgaggatc cgacgttcat ggccgcctgg ttcgggttgc
     4261 tcgtgacggc gctcaacctt ctcccgatcg gccagctcga cggcggccac gtcgtgcgag
     4321 cggcactcgg ccgacgccag ccgatcttct cctacgcggt gctcgtcctg gcggcgctct
     4381 ccgccacgag ggggccgatc tgggcattct tctgtttctt caccgcgctg ttcctcggcg
     4441 ttcggcatcc ccccgtcgag aacgacgacg agccgctgcc cttcaaccac cagatgctcg
     4501 cgctcttctg cctggcggtg ttcctgctct gcttcactct cgtcccgatg aaagtggttt
     4561 gaggggtcag gtctctgggg tcaggtctcc acttgccaca tctggggtca ggtctccact
     4621 tgccacatcg cgtggtgatg tggcaagggc ggttcggctg gccgacccca cgcggcggct
     4681 cgtgaagacc tgaccccgct cagacttgga ggatgaacgt cgccggaccg tcgttgacga
     4741 gcgacacctg catcatcgag ccgaaccgcc cggtctcgac cgacacgccg cggcgggcga
     4801 gggcttcgac gaacctctcg tagagcggtc gcgcttcctc tccggcgagc gctctctcga
     4861 agcccggccg acggccgcgg gaaaggtccg cgccgagcgt gaactgcgac acgacgagga
     4921 cgctcccacc gatggcggca agatcgaggt tcatcttccc gccctcgtcg ttgaacacgc
     4981 gcaaccccgc aacctttcgt gccgcctcgt cggccgccgc atcgtcgtcc cccttctccg
     5041 cgccgaccag gacgagcagg cctcgcccga tccgaccgac ggtctcgtcg cccacgtgaa
     5101 cctccgcctc cgaaacgcgt tggatgacga gcttcacgag gactttgatt gcccacggcg
     5161 ctgcgtgagc aggcggacga actcgcgcgg ctcgggcgag cggagtgcga gcgcgaccgc
     5221 gagatagacg atcatgccgg cgaacgtcgc ggcgacgaga aagaggaggc gggtccccgc
     5281 ggtgcgcgga gggtgagcgg cgagcatcca cgcagccgcc cacccggcga cgagcgccat
     5341 cacggccgcg gcgacgagta cgcgcaggag cgacgccgcg acttctttga ggccgagcct
     5401 cccttcgcgg cgccggagag atgccaggag caggacgaag ttcgcgatcg cgccgcagga
     5461 ggtcgcgagc gccacggacc cgactcccca gggccgcagg aggag
//
LOCUS       DASWAV010000178         3752 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U2665
            k141_289515, whole genome shotgun sequence.
ACCESSION   DASWAV010000178 DASWAV010000000
VERSION     DASWAV010000178.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960316
            Sequence Read Archive: SRR10260007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 3752)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3752)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:47:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,929
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3752
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_289515"
                     /isolate="SMAG_U2665"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.36444 N 132.31889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1334)
                     /locus_tag="VF958_03485"
     CDS             complement(<1..1334)
                     /locus_tag="VF958_03485"
                     /inference="COORDINATES: protein motif:HMM:NF014565.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004576 - oligosaccharyl transferase
                     activity [Evidence IEA];
                     GO_process: GO:0006486 - protein glycosylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STT3 domain-containing protein"
                     /protein_id="HEX9148201.1"
                     /translation="MSRSRVLFPLGVAVAVLLGTGLRLGTYRQLQDGSRTRAVSSDDY
                     YHLRRARFAVAHFPRTIFFDPLMNFPAGGVPIWPPLYDLSLAAPSLLLHGPQAPEAAV
                     EREAAGVPLLFAAGTIVLAGLLARRIYGPLAGVATALFVAVSPGHILWSQYGHVEQHV
                     AESFFGVAALLAYLASRDRPSAPGNARREAAAGVTLALAVLAWQGAVYWGAIFALALF
                     LESLRTRATVFRAAVLTLALPAALVALATAAWTRGIEMPFTYVSFGFFQPIFLAALAA
                     GVVFLDTAVGVARRGIPRRDFLLRAAFLAAALVAVLPFAGPMVESFAGGVGYVAGRTR
                     EVSGRGGYVSYPSAWLKGIFEARPLFADGILPPIRQLSLGLFLVPVAIFAWTRRALAR
                     RRTGVYVALGVWGVVTVFLALAQRVNVYYAVPLCALALVEVARLAATMVRRR"
     gene            1370..2137
                     /locus_tag="VF958_03490"
     CDS             1370..2137
                     /locus_tag="VF958_03490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019499851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEX9148202.1"
                     /translation="MKLSVLIPVYNEERTIDEILRLVARVPIEKEILVVDDGSIDGTR
                     EILKKWDGKDGVRVILHPRNFGKGRAVKTAMGEARGEILVIQDADLEYDPAEYPVLLR
                     PIEGGRADVVYGSRFFGSSERRVQFFWHTVGNRLLTLISNVFTNLNLTDMATCYKAFH
                     RRVVPALDLESKRFGVDAEFTVKVARAGFRIFEVPISYFGRSMAEGKKIRLKDGFSAL
                     SALVKHSFAPQPVRSRQVPAPGEEAGSRESAAGERPA"
     gene            2163..>3752
                     /locus_tag="VF958_03495"
     CDS             2163..>3752
                     /locus_tag="VF958_03495"
                     /inference="COORDINATES: protein motif:HMM:NF014425.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000030 - mannosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006493 - protein O-linked glycosylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid carrier-dependent
                     glycosyltransferase"
                     /protein_id="HEX9148203.1"
                     /translation="MKIRAATAVAAVALVFLSVFLVRDHTRDDSLSADEPVHILSGWF
                     EVVGRNAIVNIEHPPVAKMLAGLALSTLPLPPPPAAVPMGTQFTDFGHAFLFENRVSP
                     DVIAAAARAPFLGVLAVLLLLIFGAATSRYGAAAGLFALALLALDPNFVAHAGVVHTD
                     VAAAVAFLASVLAWEAARRRPTPLRLLAAALVLGLSLATKFSAVLLLPILLLQSLLAA
                     RREPRPGRAAAVAAVRLGAVAMVALIVVVAIYAPLTSRMDPEYQRRVIHEMVAGRGAP
                     RLSAAIESLAGVSRPLAHYLGGLASVVRQSNVGGGVNYLAGEASVHGFPSYFFVAFSL
                     KSTLAFLAVTALALVVFFRRPRESAEESRLFLLPVAVLFLASIGSAYNIGIRHMLPVY
                     PFLALAGAAVFARAWRPRDGRSRALGAMILGLLPLVSAYELLRIHPHELSYFNAFAGG
                     PERGRRILSDSNVDWGLDLKRLASELSRRGVVDPTIVYFGGDDVLYRTGVPDFAADPR
                     VRGRLVAISAFQLAVGPEFHEY"
BASE COUNT          570 a         1313 c         1280 g          589 t
ORIGIN      
        1 aagcgtcggc ggaccatcgt cgccgccagc cgcgcaacct cgacgagtgc gagcgcgcaa
       61 agcgggacgg cgtagtagac gttcacccgc tgtgccaagg ccaggaagac cgtcacgacg
      121 ccccagaccc cgagcgcaac gtaaaccccg gtgcgtcggc gcgcgagggc acgccgggtc
      181 cacgcgaaga tggccaccgg aaccaggaac aggccgagcg agagctgccg gatcggcgga
      241 aggatcccgt cggcgaagag cggccgcgct tcgaagatcc ccttcagcca ggcgctcgga
      301 taggagacgt agccgccgcg cccggagacc tcgcgcgtgc ggccggcgac gtagccgacc
      361 cctcccgcga aactttcgac catcggaccg gcgaacggga ggacggcgac gagtgccgcc
      421 gccagaaaag ccgcccgaag gagaaagtcg cggcgcggaa ttccgcgacg cgcgacaccg
      481 acggccgtgt cgaggaagac gacgcccgcg gcgagcgccg cgaggaagat cggctggaag
      541 aaaccgaacg acacgtaggt gaaaggcatc tcgattccac gcgtccacgc cgcggtcgcg
      601 agggcgacca gcgcggctgg aagagcgagg gtgaggacgg cggcgcggaa gacggtcgca
      661 cgcgtccgga gcgactcgag gaagagcgcc agcgcgaaga tcgcacccca gtagaccgcg
      721 ccctgccagg cgagcaccgc gagtgcgagc gtgacaccgg ccgccgcctc gcgccgggcg
      781 tttccgggcg ccgagggtcg gtcgcggctg gcgaggtagg ccaggagcgc tgcgaccccg
      841 aagaacgatt ccgcgacgtg ctgctcgacg tggccgtact gcgaccagag gatgtgtccc
      901 ggagagacgg cgacgaacag ggccgtggcg acgccggcga gcggcccgta gatccgcctc
      961 gccagaagcc ccgcgaggac gatcgtcccg gcggcgaaga ggagcggcac cccggccgct
     1021 tcgcgctcga ctgcggcttc cggcgcctgc ggtccgtgca gcagaagcga gggcgccgcg
     1081 agcgaaaggt cgtagagcgg tggccagatg gggactccgc ccgccgggaa gttcatcaga
     1141 gggtcgaaga agatcgtccg cgggaagtgg gcgaccgcga accgggcgcg gcgaaggtgg
     1201 taatagtcgt cgctcgatac cgcccgcgtc cgcgagccgt cctggagctg gcgataggtg
     1261 ccgaggcgca gacccgtccc gaggaggacg gcaacggcga cgccgagggg gaacaggact
     1321 cggcttcgcg acatcgacca attatgaagc cggtatcatg gagcgaagcg tgaaactgtc
     1381 ggtcctcatc ccggtctaca acgaggagcg cacgatcgac gagatcctcc gtctcgtcgc
     1441 gcgggttccc atcgaaaaag agatcctcgt cgtcgacgac ggttcgatcg acggcacccg
     1501 cgagatcctg aaaaagtggg acggcaagga cggcgtgcgc gtgatcctcc acccgcgcaa
     1561 cttcggcaag ggccgcgccg tcaagacggc gatgggagag gcgcgcgggg aaatcctcgt
     1621 gatccaggac gcggacctcg agtacgaccc cgccgagtac cccgtcctgt tgaggccaat
     1681 cgaggggggt cgcgcggacg tcgtctacgg gtcccgcttc ttcgggagct ccgagcgtcg
     1741 ggtgcagttc ttctggcaca ccgtggggaa ccggctcctg acgctcatct ccaacgtctt
     1801 cacgaacctg aacctgacgg acatggcgac ctgctacaag gcgttccacc ggcgtgtcgt
     1861 cccggccctc gacctcgagt ccaagcggtt cggcgtcgac gcggagttca ccgtcaaggt
     1921 cgcccgcgcg ggattccgga tcttcgaggt gcccatctcc tacttcgggc ggtcgatggc
     1981 ggaaggcaag aagatccgtt tgaaggacgg gttctccgcg ctctccgcgc tcgtcaagca
     2041 ctccttcgcg ccgcagccgg tgcggtcgcg acaggttccc gcgccgggcg aggaagcggg
     2101 ctcgcgcgaa tcggccgcgg gggaacggcc ggcctgacac cggcgacggc ggcgaacctc
     2161 cggtgaagat tcgcgcggcg acggccgtgg ccgccgtggc gctcgttttt ctttccgttt
     2221 tcctcgtccg ggatcacacg cgggacgact ccctttcggc cgacgagccc gttcacattc
     2281 tttccggctg gttcgaggtc gtgggtcgca acgcgattgt caacatcgag cacccgccgg
     2341 tcgccaagat gctcgccggc ctggcgctct ccacgctgcc gctgccacct ccgcccgcag
     2401 ccgtcccgat gggcacgcag ttcaccgact tcggccacgc gtttcttttc gagaaccgcg
     2461 tatcgcccga cgtgatcgcc gcggccgcga gggcgccgtt cctcggcgtg ctggcggttc
     2521 tccttctcct gatcttcggc gcggcgacgt ctcgctatgg ggccgccgcg gggctcttcg
     2581 cgctcgcgct cctcgccctc gacccgaact tcgttgcgca cgccggcgtc gtgcacaccg
     2641 acgtcgccgc ggcggtcgcc ttcctcgcga gcgtcctggc ctgggaggcc gcgcggcggc
     2701 gtccgacgcc tctccgactc cttgcggccg cgctcgttct cggcctctcc ctcgccacga
     2761 agttctcggc ggtcctgctc cttccgatcc tcctcctgca gtcgctcctc gccgcgaggc
     2821 gggagccccg tcccggccgc gccgcggctg tggcggccgt ccggctcgga gcggtcgcca
     2881 tggtcgctct catcgtcgtc gttgcgatct acgcgccgtt gacctcccgc atggatcccg
     2941 agtatcagcg tcgggtcatc cacgagatgg tcgcgggccg cggggcgccg cgcctctcgg
     3001 cagcgatcga gtcgctcgcc ggagtctcac ggccgctcgc acactacctc ggaggtctcg
     3061 cgtcggtcgt acggcagagc aacgtcggcg gcggggtgaa ttacctcgcc ggagaggcct
     3121 ccgtccacgg gtttccctcg tacttcttcg tcgccttttc gctcaagagc acgctcgctt
     3181 ttctcgcggt caccgcgctc gcgctcgtcg tgttctttcg tcgcccccgc gagtctgccg
     3241 aggagagccg tctcttcctg ctccccgtcg ccgtgctctt cctcgcttcg atcggatcgg
     3301 cttacaacat cggtatccgc cacatgctcc ccgtgtatcc ctttctcgcg ctcgcggggg
     3361 cagcggtttt cgcgcgcgcg tggcgcccgc gggacggtcg cagccgggcg ctcggagcca
     3421 tgatccttgg gcttctgcct ctggtctcgg cgtacgagct cctccggatc catccgcacg
     3481 agctgtcgta cttcaacgcg ttcgcgggcg gccccgagcg cggccgtcgg atcctttcgg
     3541 actcgaacgt cgactggggc ctcgacctga agaggctcgc gtcggagctg tcccgacgcg
     3601 gagtcgtcga tccgacgatc gtgtatttcg ggggggacga cgtgctctat cgcaccggag
     3661 tgcccgactt cgccgcggat ccccgagtcc ggggccgact cgtcgcgatc tccgcgtttc
     3721 agctcgccgt cggacccgag ttccacgagt at
//
LOCUS       DASWAV010000179         8258 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U2665
            k141_490814, whole genome shotgun sequence.
ACCESSION   DASWAV010000179 DASWAV010000000
VERSION     DASWAV010000179.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960316
            Sequence Read Archive: SRR10260007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 8258)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8258)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:47:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,929
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8258
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_490814"
                     /isolate="SMAG_U2665"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.36444 N 132.31889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2466
                     /locus_tag="VF958_03500"
     CDS             <1..2466
                     /locus_tag="VF958_03500"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX9148204.1"
                     /translation="DERLRQPPLMTYEVFDPSGASRSSFSLIPEEGRGSAAALPNEVV
                     IDRYRLAILRRSVPLSYSGAPWGRVEIEVADWPSWDPLPPRIDVYRRLVLGARGGSDD
                     GPQPPRPVLASYAPDGSPREEGPALPTDAVQKIRHGAAAVRVRLADRGTELFGEIRAL
                     PEGFRLVAIPVPDFLGRALTAALLIPGIAVLFLLLGALAVWRFLATPREAGAKILPRG
                     LRTFRGRLVGLFVVGVMIPLLAVTFFLRSAIETRTARATLDHARTALETARRVLDDYL
                     PSAAAGRGRLGLLDDPLLGWLANAVGYDLSVYAPDGQLVATSRRDLYDAGLLPERVPA
                     SIYRAIGLSGVRQQIGSRIVGGRRFEEMATGLTAVPGVPGVRSPGLLSLLLLPQQRVA
                     VAEAAQLTAAVSAFSLLVFLISAAVAGRLALRVARPVADLVEGTRAVARGNFSPQLDE
                     PPDEELRELVRAFLSMSRSLKEQTDALSREKERLATLLANLTAGVVAYRADGAVLLAN
                     PAAASLGGGRADGASLEDVFPGEKMSDVRRTLERFSDSFVGEEVEPRSGERWRIVTVP
                     LPVGGEGARMAVIEDVSEVVRSNRLAAWAEMARIIAHEIKNPLTPIRLSVEHLREVWR
                     RSGGATPEFERVLDECVTNVLAQTEELKRAASEFSDYARLPRPEIGPTDVGRLMRDSA
                     AAFAGAPGVRWAVRSEPEVLAEADPRLLSRVLANLIGNAVDALSGGAGEIALSAERRA
                     ARIVVTVEDSGPGVPDRILPRLFDPYFSAKSGGTGLGLAIAKKIVEEHGGTISAENRH
                     GGGLRVRFDLPQAAAREAPVATS"
     gene            2463..3974
                     /locus_tag="VF958_03505"
     CDS             2463..3974
                     /locus_tag="VF958_03505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9148205.1"
                     /translation="MRRSPRAIVLAVLLTVVGGCSRRTGAPKPAPPLSGDAVWFADGL
                     GAGDVAIEETLARFHCAAVYLPARLLTRGTAGWSGSDRPAPVRALSRVPVVLVVSATE
                     DPFSGAGKKRDRELGGLLAREVGAALAREREFGRVRGVHLDLPFSAATAETYAAALRE
                     ARSQLSHLLARGGATSGAPARNVPISISLRIAAPAEEKEQKAVRALASRSDGIVAFVF
                     GKEKDADPAFVDALGKVWWAGYESASGGVVRRSSGDPGPRVPEAALDPLTDDPRTELV
                     LEVPWNEGRGSQFTLRAMRALTVAGVSLAAGDSVVFTEPSLPDLVSAFRQGTSGRRLA
                     RGRILVVGGEADDGRIFPVAALDDVIGGRRAAPKFSAWTAAEGSRLLRVGADNPSPHA
                     SVVSRIENWIDVDFAPARIGDVELGGFDRWEAHDEGGRPVSPGRATRVRLFETFVAPF
                     ERIEPARLRARGNLPSSCCRMRTHVGPAAGGEVATDWEEPGALSGAAKPTPSP"
     gene            complement(3999..4844)
                     /gene="pssA"
                     /locus_tag="VF958_03510"
     CDS             complement(3999..4844)
                     /gene="pssA"
                     /locus_tag="VF958_03510"
                     /EC_number="2.7.8.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR00473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003882 - CDP-diacylglycerol-serine
                     O-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--serine
                     O-phosphatidyltransferase"
                     /protein_id="HEX9148206.1"
                     /translation="MARKAEPGDGNRNREPTDAFLFPPPAGPEDSPGPRLRTRARQRL
                     SRGLFILPTLFTVGNLFCGYLSVWCSIRGTFDTAAILIIAAAVLDMLDGRIARLTNSA
                     SRFGEEYDSLADLVSFGVAPAVLAYAWGLADFHRLGWMASFLFVVCGSMRLARFNIQT
                     RVVDKKYFIGLPIPAAAGTIATLVLATPERLVDQVWMAGLLALTVVLSYLMISTLRYR
                     SFKDLDLKRQRRAWILPVIALIFAVIAYRPAAALLAMALVFAASGPVAKLVGLLRDRQ
                     RKASA"
     gene            complement(4856..5479)
                     /locus_tag="VF958_03515"
     CDS             complement(4856..5479)
                     /locus_tag="VF958_03515"
                     /EC_number="4.1.1.65"
                     /inference="COORDINATES: protein motif:HMM:NF003678.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004609 - phosphatidylserine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylserine decarboxylase family protein"
                     /protein_id="HEX9148207.1"
                     /translation="MPVAREGWSFILPPVALAAIAALTGHPIIAFVLFVLALFLLSFF
                     RDPERVPEGGEETIVSPADGTVLSVAPAPEAPAGASRRLSIFMSVFNVHVNRAPVSGE
                     IADYVYSPGRLRAAFQEKASTENEQNRITLASPRGGVTFKQIAGAVARRIVFYPRVGE
                     RLWRGQRIGIIRLGSRVDLFIPDDAEVLIARGDKVRAGKTAIARWKA"
     gene            complement(5482..5706)
                     /locus_tag="VF958_03520"
     CDS             complement(5482..5706)
                     /locus_tag="VF958_03520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcH family protein"
                     /protein_id="HEX9148208.1"
                     /translation="MESDHTEAIPETDPEYRRLHDEHRDHEYRLQILAAKSRLSEDEE
                     IEEKRLKKEKLLIKDRMEAIARNYREGVAH"
     gene            complement(5805..6287)
                     /gene="rimI"
                     /locus_tag="VF958_03525"
     CDS             complement(5805..6287)
                     /gene="rimI"
                     /locus_tag="VF958_03525"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: protein motif:HMM:TIGR01575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008999 -
                     peptide-alanine-alpha-N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HEX9148209.1"
                     /translation="MALRKPEASLYSIRPARPADLDEIARIERASFRIPWKREFFESE
                     LSEPYRYARVLSGEGIEPPIGGYLFAVSLYEEFHVNKIATDPAVRQRGYGRMLIEDAI
                     AHARGMRAASMTLEVRVSNLPAREFYRSYGFREAFRRKSYYQDGEDAYALVLPLSPAR
                     "
     gene            complement(6274..6912)
                     /gene="tsaB"
                     /locus_tag="VF958_03530"
     CDS             complement(6274..6912)
                     /gene="tsaB"
                     /locus_tag="VF958_03530"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: protein motif:HMM:TIGR03725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HEX9148210.1"
                     /translation="MQILALDTASPIPSVTLLAAGGGVWEQPIPQDRRASEELLPAIE
                     RCFASASTSLPECDRIAVCSGPGSFTGLRIGLATAWALGRATGVAVETASTLEALAES
                     IRGRGASRVAAALDAGREEVVLAFYSLEGKRAQLESEPVRIPLEVARERAAGVPVISM
                     PPDLVTPRGEPAEVSLSRALAFAVARAPGKTLSRLAGMYSRPSAAEERHGSP"
     gene            6960..>8258
                     /locus_tag="VF958_03535"
     CDS             6960..>8258
                     /locus_tag="VF958_03535"
                     /inference="COORDINATES: protein motif:HMM:NF017043.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HEX9148211.1"
                     /translation="MSSSPPRVTPMLRQYFEMKSRVPDAILFYRMGDFYEMFFEDARE
                     AAPLLGITLTARHRDSDIEAPMCGVPWQSADQHIARLVAAGRKVAVCDQVEDPRAAKG
                     LVRRDITRIVTPGTVLDPEALLPDAPCWLAALAPGPEEWGIALLDMSTGRFHAGRVLP
                     GRLGEVFALFRPREALLPDGFELPFQSDVTITRRPPDWFRLGRADVTFQRLPPEDSAG
                     RAAATAARAYAAEMRPRGLPHLLQPEPLRFDQRMRLDPSAVATLELFEASDGDRRRSL
                     CALVDRTRTPMGARSLKDAISRPALDPIELTERWDAVEELVAKPDARERLRHELEGIG
                     DLERQFGRIAVGTAGPRDVLAWSAGLRRLPGVRTAGEALVSPRLRLLLDQIPDVGDLA
                     DRVERTIAPDAPALASAGGVLRAGADAELDSLRELRRGAQG"
BASE COUNT         1203 a         2875 c         2900 g         1280 t
ORIGIN      
        1 gacgagcgtc tgcgtcagcc gccgctcatg acgtacgagg tcttcgaccc gtcgggagcg
       61 tcacggagca gcttttcgct catccccgag gaagggagag gctccgcggc ggctctcccg
      121 aacgaggtcg tcatcgaccg ataccggctc gcaatccttc gccgctcggt cccgctctcc
      181 tactcgggag cgccgtgggg acgggtcgag atcgaggtgg cggactggcc gagctgggac
      241 ccgctgccgc cacgcatcga cgtctatcgg cgactcgttc tcggcgctcg cgggggatcg
      301 gacgacggtc cccagcctcc gcgtcccgtc ctcgcctctt acgctccgga cggctcgccg
      361 cgcgaggagg ggccggcgct tccgaccgac gcggtgcaga agatccggca cggcgccgcc
      421 gcggtccgcg tgcgcctcgc ggaccggggg accgaactct tcggcgaaat ccgcgcactg
      481 cccgaaggct ttcgtctcgt ggcgatcccg gtgccggact tcctcgggcg agccctgacc
      541 gccgcccttc tcatacccgg catcgcggtg ctcttcctgc tgctcggcgc actcgcggtg
      601 tggagatttc ttgcgacgcc ccgggaagcg ggggcgaaaa tcctcccccg cgggttgcgc
      661 acgttccggg ggcggctcgt cggactgttc gtcgtcggag tcatgatccc cctcctcgcc
      721 gtgacgttct tcctgcgctc cgcgatcgag acgcggaccg cgcgggccac gctcgatcat
      781 gcgcgcaccg cgctcgagac ggcgcggcgc gttctcgacg actatctccc ttcggcggcg
      841 gccgggcgag gccgcctcgg gctcctggac gatccgcttc tcggctggct cgcgaacgcg
      901 gtcggatacg acctctccgt gtacgcgccc gacggtcagc tcgttgcgac ctcacggcgc
      961 gatctctacg acgcgggcct gttgcccgag cgcgtgcccg cctccatcta tcgcgcgatc
     1021 ggactttcgg gcgtccggca gcagatcgga tcgcggatcg tcggcggccg gaggttcgag
     1081 gagatggcca ccggtctcac ggccgttccg ggagttccgg gcgtccgaag tccgggcctc
     1141 ctctcgctac ttctcctccc gcagcagcgc gtcgccgtgg cggaggcggc gcaactgacg
     1201 gccgcggtat ccgcgttcag cctcctggtg tttctcatct ctgccgccgt cgcggggcgt
     1261 ctcgcgctcc gggtcgcgcg cccggtcgcg gatctcgtcg agggcacccg cgccgtcgcg
     1321 cgcggaaact tctctccgca gctcgacgag ccgccggacg aagagctgcg cgagctcgtt
     1381 cgcgcgttcc tctcgatgtc gcgaagcctg aaagagcaga ccgacgcgct ctcgcgggag
     1441 aaggaacggc tcgcgacgct gctcgcgaac ctgaccgcag gcgtcgtggc gtaccgcgcg
     1501 gacggagcgg tgctcctcgc caatccggcg gcggcgtccc tcggcggcgg gcgggcggat
     1561 ggtgcgtcgc tcgaggacgt cttcccggga gaaaagatgt cggacgtccg gcgcacgctc
     1621 gaacgcttct cggactcgtt cgtcggggag gaggtcgagc cccggtccgg agaacgatgg
     1681 cgcatcgtga ccgtcccgct tcccgtcgga ggcgagggtg cgcgcatggc ggtcatcgag
     1741 gacgtatccg aggtcgtgcg ctcgaaccgg ctcgccgcgt gggcggaaat ggcgcggatc
     1801 atcgcgcacg agatcaagaa cccgctcacc cccatccggc tctccgtcga gcacctgcgc
     1861 gaggtgtggc ggcgcagcgg cggcgccaca cccgagttcg agcgggtcct cgacgagtgc
     1921 gtcaccaacg tcctcgcgca gacggaagag ttgaagcgcg cggcctcgga gttctcggac
     1981 tacgcgcgtc tcccgcgccc cgagatcggc ccgaccgacg tgggtcgcct catgcgagac
     2041 agcgctgccg cgtttgccgg agctcccggc gttcgctggg cagttcgatc cgagccggag
     2101 gtgctcgccg aggcggaccc ccggctgctg tcgcgtgtcc tcgcgaacct gatcgggaac
     2161 gccgtcgatg cgctctccgg cggggcgggg gagatcgcgc tctccgcaga acgaagagcc
     2221 gcgagaatcg tggtcacggt ggaagactcc ggacccggtg tgcccgaccg aatcctgccg
     2281 cgcctcttcg atccgtattt ttcggcgaag agcggcggga ccgggctcgg tctcgcaatc
     2341 gcgaaaaaga tcgtcgaaga gcacggcgga accatctccg cggagaaccg ccacggcggc
     2401 ggcttgcggg tgcggttcga tctccctcag gcggccgcgc gcgaggcgcc ggtggcaacg
     2461 tcgtgaggcg atctccccgc gcgatcgtgc tcgccgttct gctcaccgtc gttggcggat
     2521 gctcgcgtcg caccggagcg ccgaagccgg cgccgccgct ctcgggggat gccgtctggt
     2581 tcgccgacgg cctcggcgcg ggagacgtcg cgatcgagga gacgctcgcg cgctttcact
     2641 gcgccgcggt ttaccttcct gcacgcctcc tcacgcgcgg gacggcggga tggtccggat
     2701 ccgaccgtcc cgcccccgtc cgggcgctgt cccgagtccc ggtcgtgctc gtcgtctccg
     2761 cgacggagga cccgttctcc ggcgcgggca agaagcgcga ccgggagctc ggcggcctgc
     2821 tcgcgcgcga ggtcggggcg gcgctcgccc gggagcgcga gttcgggcga gtccgcggcg
     2881 tccacctcga cctgccgttt tcggcagcga ccgcggaaac gtacgcggcc gcgctccggg
     2941 aggcgcgctc gcagctttcc cacctcctcg cgcgcggggg cgcgacgtcg ggtgcaccgg
     3001 cacgaaacgt ccccatctcg atctcccttc ggatcgcggc gccggcggaa gagaaggagc
     3061 aaaaggcggt gcgcgctctc gcctcgcgca gtgacgggat cgtcgcgttc gtcttcggga
     3121 aggagaagga cgccgacccc gcgttcgtcg atgcgctcgg caaggtctgg tgggccggct
     3181 acgagtccgc cagcggaggg gtcgtccggc ggtcctcggg ggatccgggg cccagggtac
     3241 cggaagccgc cctcgatccc ctgaccgacg acccgcggac cgagctcgtc ctcgaggttc
     3301 cctggaacga ggggcgcggc tcgcagttca cgctccgggc gatgcgcgcg ctcacagtgg
     3361 cgggcgtctc gctcgccgcg ggcgactccg tcgtgttcac ggaaccttct ctccccgatc
     3421 tcgtgtcggc tttccggcag ggcacgtcgg ggcgccggct cgcacgcggc cggatcctcg
     3481 tcgtcggtgg ggaagccgac gacggccgga tcttcccggt tgcggctctc gacgacgtca
     3541 tcggcgggcg ccgcgcggca ccgaagttct ccgcctggac tgcggctgaa ggttcgcgac
     3601 tcctgcgtgt cggcgcggac aatccctccc cccacgcgtc ggtcgtctcg cggatcgaga
     3661 actggatcga cgtggatttc gctcccgccc gcatcggtga cgtcgagctc ggcggcttcg
     3721 accgctggga agcgcacgac gaagggggac gccccgtatc gccgggccgg gccacccgtg
     3781 tccggctctt cgagacgttc gtagcaccgt tcgagcggat cgagcctgcg cggctccgcg
     3841 cgcgcggaaa cctcccgtca tcctgctgcc ggatgcggac gcacgtcggg cccgcggcag
     3901 gaggcgaggt ggcgacggac tgggaagagc cgggagctct cagcggagcg gcgaagccaa
     3961 ccccatcacc ctgacctttt cccccaggaa gagggaactc aggccgacgc ttttcgctgt
     4021 cggtctcgca gcaggcccac gagcttggcc accggccccg aagcagcgaa cacgagcgcc
     4081 atcgcgagga gagcggcggc cggacgatac gcgatcacgg cgaagatgag cgcaatcacg
     4141 ggaagaatcc acgcgcggcg ctggcgcttc aggtcgagat ccttgaacga gcggtaacgg
     4201 agcgtcgaga tcatgagata cgacagcacg accgtgagcg cgaggagccc ggccatccag
     4261 acctggtcga ccagacgctc gggggtggcg agcacgagcg tcgcgatcgt ccccgccgcc
     4321 gcgggaatcg gcagaccgat gaagtacttc ttgtcgacga cgcgggtctg gatgttgaag
     4381 cgcgccagac gcatcgaacc gcagacgacg aacaggaacg acgccatcca cccgaggcgg
     4441 tgaaagtcgg cgagtcccca cgcgtaggcg agtacggcgg gagcgacgcc gaacgagacg
     4501 aggtcggcga gcgagtcgta ttcctcaccg aagcgggaag ccgaattcgt caggcgggcg
     4561 atgcggccgt cgagcatgtc gagcacggcg gcagcgatga tcaagatcgc cgcggtatcg
     4621 aaggtcccgc ggatcgaaca ccagacggac aggtacccgc agaacaagtt cccgacggtg
     4681 aagagggttg gaaggatgaa tagcccgcgc gacaggcgct ggcgggcgcg cgttcggagc
     4741 cgcgggcccg gggaatcttc cggcccggcg ggaggtggaa acaggaaggc atcggtcggt
     4801 tcccgatttc gattgccgtc ccccggttcc gccttacgtg ccacgcatca ccctctcagg
     4861 ccttccaacg agcgatcgca gtctttcctg cccggacttt gtctccgcgc gcgatcagga
     4921 cctccgcgtc atcggggatg aagaggtcca cgcgcgaccc cagccggatg atcccgatcc
     4981 gctggccgcg ccacaggcgt tcgcccaccc tcgggtagaa cacgatccgc cgggcgaccg
     5041 ctcccgcgat ctgcttgaac gtcacgcctc cgcgcgggga tgccagcgtg atgcggttct
     5101 gctcgttctc ggtggacgcc ttctcctgga aggccgcgcg cagccggccc ggcgaataga
     5161 cgtagtcggc gatttcgccg gagacgggcg cgcgattcac gtggacgttg aagaccgaca
     5221 tgaagatcga gagcctccgc gaggcgcccg ccggcgcctc gggtgccgga gcgacggaga
     5281 ggacggtgcc gtcggcgggc gagacgatcg tttcttctcc gccttccggg acccgctcgg
     5341 gatcccggaa gaaagagagg aggaaaaggg cgagaacgaa aagaacgaaa gcgatgatcg
     5401 gatgcccggt gagtgcggcg atggccgcga gcgccacggg aggaaggatg aaagaccacc
     5461 cttcgcgggc gacgggcacc ctcagtgggc gaccccctcc cggtagtttc gggcgatcgc
     5521 ttccatgcgg tccttgatca ggagtttctc cttcttgaga cgcttttctt cgatttcttc
     5581 gtcctcggag agccgcgact tcgctgcgag gatctgtaaa cggtactcgt ggtctcggtg
     5641 ctcgtcgtga agccggcggt actccggatc cgtttctggt atggcctccg tgtgatcgct
     5701 ttccattcga tcagacctcc ttgacgtttt ttcggggaaa ggagcggcgg gcccgggtca
     5761 cggctggagt ttatcggcgg cggcagcccg tttcaacagc gttactaccg ggcgggcgag
     5821 agaggcagga cgagcgcgta ggcatcctct ccatcctggt agtacgactt cctccgaaaa
     5881 gcctcgcgaa agccgtagga gcggtagaac tccctcgccg ggaggttcga tacgcgcacc
     5941 tcgagggtca tgctggccgc ccgcatcccc cgcgcgtgcg cgatcgcgtc ttcgatcaac
     6001 atgcggccgt agccgcgctg gcggacggcc ggatcggtgg cgatcttgtt gacgtggaat
     6061 tcttcgtaca gggagaccgc gaagagatat ccgccgatcg gcggctcgat gccctctccg
     6121 gagagaaccc gcgcgtatcg gtacggttcg gagagctcgc tctcgaagaa ctcccgcttc
     6181 caggggatcc gaaaggatgc gcgctcgatg cgcgcgatct cgtccaggtc ggcgggccgg
     6241 gccggccgga tcgaatagag ggacgcctcg ggcttacgga gagccatgtc tctcctccgc
     6301 cgcgctcggc cgcgagtaca tcccggcgag gcgcgacagc gttttgccgg gagcgcgagc
     6361 gacggcgaac gcgagcgcgc gggagagcga gacctcggct ggttctcccc gcggcgtgac
     6421 gaggtccggg ggcatcgaga tcaccgggac cccggcggct cgctcccgcg cgacctcgag
     6481 agggatccgc acgggttccg actccagctg ggcgcgcttt ccctcgagcg aatagaacgc
     6541 gaggacgacc tcctcccgcc cggcgtcgag ggccgccgcg acccgcgacg ctccgcgccc
     6601 ccggatgctt tcggcgagcg cctccagcgt cgatgcggtc tcgacggcga cacccgtcgc
     6661 gcggccgagc gcccacgccg tcgcgagccc gatgcgcagc cccgtgaacg aaccgggtcc
     6721 ggagcacacg gcaattcgat cgcattcggg aagactcgta ctcgccgagg cgaagcagcg
     6781 ctcgatcgcg ggaaggagct cctcggacgc tctgcggtcc tgggggatcg gctgctccca
     6841 cacgcccccg cccgcggcga ggagcgtgac ggagggaatg gggctcgcgg tgtcgagcgc
     6901 gagaatctgc attgcggaga aaccctcgtg agctcgggcg cgggtatgct agcacttggg
     6961 tgagctcgtc tcctccgcgc gtcacgccga tgctccggca gtacttcgag atgaagagcc
     7021 gggtccccga cgccatcctc ttctaccgga tgggcgattt ctacgagatg ttcttcgaag
     7081 acgcgcgcga agcggctccg ctcctcggga tcacgctgac ggcgcggcac cgggactcgg
     7141 acatcgaggc cccgatgtgc ggcgtgccct ggcagagcgc cgaccagcac attgcgcgcc
     7201 tggtcgccgc cggccggaag gtcgcggtct gcgatcaggt ggaggacccc cgcgccgcga
     7261 agggcctcgt ccggcgcgac atcacgcgca tcgtcacgcc cgggaccgtg ctcgaccccg
     7321 aggcgctcct tcccgacgcc ccgtgctggc tcgcggcgct cgctccgggc ccggaggagt
     7381 ggggaatcgc gttgctggac atgtccacag gccgcttcca cgccggtcgg gtgctgccgg
     7441 gacgtctcgg agaggtcttc gcactgttcc ggccgcgcga ggcgctcctc cccgacggct
     7501 ttgagctgcc gttccagtcc gacgtgacga tcacccggcg tcctccggac tggttccgcc
     7561 tcgggcgggc cgacgtgacc ttccagcgcc tcccgcccga ggactccgcc ggacgcgccg
     7621 ccgcgactgc ggcgcgcgcc tatgccgccg agatgcggcc ccgcggcctt ccgcatcttc
     7681 tccaacccga gccgcttcgc ttcgaccaac ggatgcggct cgacccgtcg gcggtggcaa
     7741 cgctcgagct cttcgaggcc tccgacggcg accgccgccg cagcctttgc gcgctcgtcg
     7801 accgtacgcg caccccgatg ggggcccgct ccctcaagga cgcgatctcg cgtcccgctc
     7861 tcgatccgat cgaactcacg gagcggtggg acgcagtcga ggagctcgtc gcgaagccgg
     7921 acgcgcgaga gagactccgg cacgagctcg agggcatcgg ggacctcgag cgtcagttcg
     7981 gccgtattgc ggtcgggacc gcaggtccgc gcgacgtcct cgcgtggagc gccggtcttc
     8041 ggcggctgcc gggcgttcgc acggccgggg aggcgctcgt ttcgcctcgc cttcgccttc
     8101 tgctcgacca gatacccgac gtcggagacc tcgcggatcg ggtggagcgc acgatcgccc
     8161 ccgacgcccc ggcgctcgcc tccgcgggag gcgtgctgcg cgccggcgcc gacgccgaac
     8221 tcgactcgct ccgggagctg cgccgcggcg cgcagggg
//
LOCUS       DASWAV010000180        13263 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U2665
            k141_90790, whole genome shotgun sequence.
ACCESSION   DASWAV010000180 DASWAV010000000
VERSION     DASWAV010000180.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960316
            Sequence Read Archive: SRR10260007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 13263)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13263)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:47:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,929
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13263
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_90790"
                     /isolate="SMAG_U2665"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.36444 N 132.31889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1362
                     /locus_tag="VF958_03540"
     CDS             <1..1362
                     /locus_tag="VF958_03540"
                     /inference="COORDINATES: protein motif:HMM:NF019229.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRTAC1 family protein"
                     /protein_id="HEX9148212.1"
                     /translation="NRGDGTFEEVGERAGVALNDYSTSAAALDYDRDGDADLYVLVYG
                     PPGKGPNVAANNAPPNHMYRNNGDGTFTDVSRETRTADTRWGLALQAADLDADGWPDL
                     YVANDFGVNTYLRNRGNGTFENAAAGANVSDPGFGMGVTVDDSDGDGRLDFYVSNYSF
                     PLSWFLRDRHFPMPQFPYWLGRPLVWRRLKAMTRGSSLFLARPDGRFERTSDEADVWD
                     TSWSWGCVFVDADLDGRPDLFVVNGMVTGKNLTEREIDFWNLMSYDYKKFEKGTSTTD
                     FGDDSLWGRPPKRFYRNVDGQRFVELAAVTGLESQANERGLVVFDADGDGSPDLFATG
                     FLSKPSLWVNRNPSHARSLVITLQGLPDAKTRYRTTRDALGAVVTVEAAGWKRSQVVS
                     AGYSFLSSGSKSLFFGLGDAPRADRVTIRWPSRKVSELRDVAAGKISLGEPMDGETLT
                     RSR"
     gene            1399..2103
                     /locus_tag="VF958_03545"
     CDS             1399..2103
                     /locus_tag="VF958_03545"
                     /inference="COORDINATES: protein motif:HMM:NF025729.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4412 domain-containing protein"
                     /protein_id="HEX9148213.1"
                     /translation="MIRRLTLTLSMAAALTLPLAAEDLTIVSKVTTGGRSGTSTQYMT
                     STKSRTTDGQTDSIMDYPTGTMTVIDHRNKTYWEATIQEMSAYMDKLEKDMKGNPMLE
                     NMFGGPDDVSVEKGKGSRKVAGYDCDEYTMRMGESFVFDLCAARGLQPPPQYYEGRKL
                     SSAAMGPMGKRFAKMFEEMKKIKGYPVALDMDMNMGMSKMKTTSEATEVKKGSIPAST
                     FEIPSGYEKKASPFKR"
     gene            complement(2111..4375)
                     /locus_tag="VF958_03550"
     CDS             complement(2111..4375)
                     /locus_tag="VF958_03550"
                     /EC_number="1.1.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012647954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004470 - malic enzyme activity [Evidence
                     IEA];
                     GO_function: GO:0004471 - malate dehydrogenase
                     (decarboxylating) (NAD+) activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent malic enzyme"
                     /protein_id="HEX9148214.1"
                     /translation="MTREDALNYHSRGRKGKVEVIPSKPCRTQEDLSLAYTPGVAEPC
                     LEIARDPETVYEYTTKGNLVAVISNGTAVLGLGDIGPLAGKPVMEGKGVLFKRFADID
                     VFDIEVDAKDPAAFIDTVARISPTFGGINLEDVKAPECFVIEEELKKRLKIPVFHDDQ
                     HGTAIISAAALLNALEIAGKKIEDAKVVFMGAGASATACARLYLSYGVRKENLRMVDR
                     SGVIYVGRTADMNDYKAQFAVETKERTLIDALQGADVLVGLSAKGLVQGEMLKGMARD
                     PIVFALANPEPEITPEEAKAARPDVIMATGRSDYPNQVNNVLGFPFIFRGALDVRATE
                     INEPMKKAASQALAALAKMDVPDSVSKAYGGQEFRFGRDYLIPKPFDFRVLLWVAPAV
                     AEAAMASGVARKPITDMAAYLRRLEAMLSRAREVMGVVIRKASQDPKRIVFPEGEHPK
                     ILRAAKILVEEGICRPILLARIEEVEPLARELQLPMDKIEFVHSRSSPRLPEYARLLE
                     ERRRRRGVTVTDALDQAGARNAFGPLMVEAGDADGVISGLTQHYPETIRPALQIVGTA
                     PGVRRVSGLYVLIFKDRTFFFADTTVNIDPTAEELAEIALLTADAVHRFDLEPRIAMI
                     SFSNFGSNTHANALKVKRAVELVKAARPDLPIDGEMQVDYAVVPELLEAEYPWANVRR
                     PNVLIFPDLQSANAAYKLVWRLAGAEAIGPILMGMRKPVHVLQRAVEVADIVNMAAIA
                     VVDAQEKEEQRPIL"
     gene            complement(4381..6114)
                     /locus_tag="VF958_03555"
     CDS             complement(4381..6114)
                     /locus_tag="VF958_03555"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015836645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HEX9148215.1"
                     /translation="MRWSRYFLQTVREVPGDAEAISHVLLTRAGMMRRIAAGIYALTP
                     LGLRSHRKTETIVREEMDRAGCLELEVPILQPRELWEEAGRWGRYTAEEILFHLKDRK
                     AGAYCLAPTAEEAVTSLVRAGVTSYRQLPLTLYQIRTKFRDEIRPRFGLMRGREFLMY
                     DGYSFDADMEGLDRSYRAQDAAYRRIFERCGLDFTVVAADSGAIGGTASEEFMVLADT
                     GEDAVARCAACGYGANVEKAETGRRPSPWDSEAPAALEDVETPGRGGIDEVVDFLGIT
                     ASRMIKCLVYETENGFVVALLRGDLDANEVALKNALGVDNLTLASEEKVERATGAPVG
                     YVGPHHLSGRDLRVVADESIRGAVNAVTGAGRRDWHVRGLSLERDAAASEWFSFSKAR
                     EGDPCPRCGKPLTIARGIEVGHIFKLGTKYSTAMRAEFTDEKGETRPIVMGTYGIGVG
                     RTMASAVEQHHDDDGIVWPLALAPFEIAIVSLNPADEAVRKEADSLYEALRSGGMDVF
                     YDDRDERPGVKFKDADLVGFPIRVNVGGRALKEGKVEVVTRRDRKVRAVPATEAASEV
                     LAAREELVART"
     gene            complement(6146..8008)
                     /locus_tag="VF958_03560"
     CDS             complement(6146..8008)
                     /locus_tag="VF958_03560"
                     /inference="COORDINATES: protein motif:HMM:NF045090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX9148216.1"
                     /translation="MFCQVCGSRNPDDEEFCVRCQSKLLVLSGVGVVEEGREVQEEIP
                     FDEHLLERISTLEEVVKRTGDALKSLFESLGNLEKNLFVAHTGVLALQEMLERRGVVR
                     AEEVRDLWETKLDERMQAVEKKDRFLERRDRIIGRFSGANSDLFTKRVREAEFALLAL
                     DADRGIRALEDLYKVDRSNTELGFYLAETFFSAGDMERASGYLKKILSVQPNHFESLV
                     YSGIVASEAGNAEEAESFHRRAIERRSDAFLPHFALGALYAQASRWNEAERELAKAVD
                     IAPQPSAHVLLGTVLREKGDVNRAIRHLEEALKLQPDSEEAVFQLGLCHLEKNRLKRA
                     LECFHEALEKNPRKLEYQEAVRILERRKFYPLPDVEGPGAEAYRRAEESVARGSLRRA
                     HELYRQALTAEPENSTIRISYALLSASLGLWTEAIASCREVLSGEPEEVVAAAACSIL
                     SEALRAEGRPAEAAQVVRDFLDGHQSSTARAVGYYELATNLAESGEDLDSALDYAGRA
                     LAAAPEELKPYPLAALGWVHYKRHELDRAIDCLRRSSERAATPTTFHQLGLAYLAAGR
                     LEDAKGAFHKAKNVASGGKLEDRMMQQVRSNLRLLEKAGPKKKESPKPKAQSPK"
     gene            complement(8011..8886)
                     /locus_tag="VF958_03565"
     CDS             complement(8011..8886)
                     /locus_tag="VF958_03565"
                     /EC_number="1.1.1.157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019461988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxybutyryl-CoA dehydrogenase"
                     /protein_id="HEX9148217.1"
                     /translation="MSPRTIGVVGAGQMGNGIAHVAAASGFEVILTDVEERFLEKARA
                     TIEKNLDREVAKGKRSAEVRSAALGRIATTTNLDRLAAASLVIEAIVENEGAKRELFQ
                     RLDAVCGPSTILASNTSSISITRLAAATRRPERFIGMHFMNPVPVMALIEMIRGIATS
                     DETAAAVERVAREMGKTPVSCNDSPGFVANRVLLPMINEAIDALREGVGTREAIDSIM
                     KLGANHPMGPLALADLIGLDTCLSILRVLQEGFGDPRYRPSPLLVKMVDAGWLGRKTG
                     KGFYEYSSEPARQRP"
     gene            complement(8922..9776)
                     /locus_tag="VF958_03570"
     CDS             complement(8922..9776)
                     /locus_tag="VF958_03570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9148218.1"
                     /translation="MIAAVAALALLLASPAAASPSGISSVGRDVVVTEKVPGRIVAVA
                     ADVRVESAVAGDVIVWGGDVSFGPAGTVAGNLVVFGGTIRGVPGRALPVGGTVSTPGT
                     LLRLYLAEMKRAPWEPGALAPVVWGLRLLALSAWLAVAVALLYFFGSPLARAADRAES
                     NWTGALTAGVLGVLTIFLAAAAALALLPSAVAVPVALALASAAIAAKVFGMGALFLLL
                     GQKLTASFSPGRRPSALAVGFAVLGGLSLLPVAGPIVWSAASVAAVGVAFVSRFGAPH
                     VRVALRAI"
     gene            complement(9773..10336)
                     /locus_tag="VF958_03575"
     CDS             complement(9773..10336)
                     /locus_tag="VF958_03575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9148219.1"
                     /translation="MNPCARPLDPIDAEALASGAEPVLAPDAAAHAASCPACGASVER
                     AKALTRALEGLAQADSPLPDLAGRVSRLRAFSARERRTYALWQWPVLLAVALAVGGLG
                     LLVGPSLSGGEQAGLSAAALAPVLGFLRSLPRWGSDLARLAPSALEALADALARERSL
                     GFASLLLLIPSAFGLTRVFARARSRRS"
     gene            complement(10333..10908)
                     /locus_tag="VF958_03580"
     CDS             complement(10333..10908)
                     /locus_tag="VF958_03580"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEX9148220.1"
                     /translation="MELDNDGVLVQRILAGEEPLFEVLVRRYQARVVAHVTRMVGSRD
                     DAFDLAQEIFLKVFQALDRYNPEFKFSTWLFRIAGNAAIDHLRKRRPRTVPLEIPDPD
                     SRSGVSSLEHESAGLDPYGQLRNLQRGEAISRAIADLPPEFRELITLRHFGGLSYEEI
                     ARVKNMPLGTVKNKLFRARVVLKERLAGELS"
     gene            11140..12360
                     /locus_tag="VF958_03585"
     CDS             11140..12360
                     /locus_tag="VF958_03585"
                     /EC_number="1.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015897940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Glu/Leu/Phe/Val dehydrogenase"
                     /protein_id="HEX9148221.1"
                     /translation="MMSRFDVAAQKLGLDPGLYKVLREPVRETKVSIPVVMDDGHIEV
                     FIGYRVLHNIARGPGKGGIRFDKNVTLDEVRALAAWMTWKCAVVNIPFGGAKGGVICD
                     PATLSRGELERITRRYTAELMDQFGPEKDVPAPDMGTNPQTMAWVMDTYSMHARHTVT
                     SVVTGKPLSLGGSRGRVEATGRGLMLIAREAAPLIGLTLSGSRVVVQGFGNVGSIAAR
                     MCHEAGARVLAVSDINGGIVNPAGLDMPAVLAHYERARTFEGFRDARKISNAELLELD
                     CDILIPAANENQIHGRNAPNIKAKIIVEGANGPTTQQADEILHQKGVLVVPDILANAG
                     GVTVSYFEWVQDRAGFFWREQEVNERLEDILVQSFHDVRSMAEKYNVSYRIAAYMLGI
                     SRVAQDTMVRGLYA"
     gene            complement(12394..>13263)
                     /locus_tag="VF958_03590"
     CDS             complement(12394..>13263)
                     /locus_tag="VF958_03590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9148222.1"
                     /translation="EPKMAVCALRFRPRLAVLSFGVLLLLASSRAFAGGSPTFLGPTP
                     YLSKADSPFIADINAGHVYLETFECGNLTVPGVTPSTGTIIPPGFEGLIDSVDADDGS
                     IDGSGLGGHSLFTGDGATGITFNFNQTTLGAFPTEVGIVWTDGGGTATFEAFDSIGAS
                     LGVIGPVAIGDASNSGETAEDRFFGVTFPGGISAIKISNTGGGLEVDHLQYGLYASAT
                     VTPCGVGLPTPTPTTTTVAATPTPTPTLAQATATATAMAGPAAAVPTLSFPMMAMLGL
                     ALAGAAILLIRGR"
BASE COUNT         2257 a         4479 c         4433 g         2094 t
ORIGIN      
        1 aacaggggcg acggcacgtt cgaggaagtg ggcgagcggg ccggcgtcgc gttgaacgac
       61 tattccactt ccgccgcggc gctcgactac gaccgcgacg gcgacgccga cctctacgtc
      121 ctcgtgtatg ggccgcccgg aaagggaccg aacgtcgccg cgaacaacgc gccgccgaac
      181 cacatgtacc gaaacaacgg cgacgggact ttcacggacg tctcgagaga gacccgcacg
      241 gccgacactc gctggggtct cgccctccag gccgccgact tggacgccga cggctggccg
      301 gacctgtacg tcgccaacga cttcggcgtg aacacgtatc ttcgcaaccg ggggaacggc
      361 accttcgaga acgccgccgc cggcgcgaac gtcagcgacc cgggcttcgg aatgggggtc
      421 acggtcgacg actccgacgg cgacggccgg ctcgacttct acgtctcgaa ctactcgttc
      481 ccgctcagct ggttcctgcg cgaccgccac ttcccgatgc cgcaattccc ctactggctc
      541 ggccggcccc tcgtctggcg ccgcttgaag gcaatgacgc gcggctcctc gctcttcctt
      601 gcgcggccgg acggacggtt cgagcgcacg tcggacgagg cggacgtgtg ggacacgtcg
      661 tggtcgtggg gctgcgtatt cgtcgatgcc gacctcgacg ggcgacccga tctcttcgtg
      721 gtcaacggga tggtcaccgg aaagaatctg accgaacgcg aaatcgactt ctggaacctg
      781 atgagctacg actacaagaa attcgagaag gggacttcga cgaccgactt cggcgacgac
      841 tcgctgtggg gccggccgcc gaagcgcttc taccggaacg tggacgggca gcggttcgtc
      901 gagctcgctg cggtcacggg tctcgaatcg caggcgaatg agcgcggcct ggtcgtcttc
      961 gacgccgacg gcgacgggag ccccgatctc ttcgcgaccg gtttcctgtc gaagccgtcg
     1021 ctctgggtga accgcaaccc gtcgcacgcg cgctcgctcg tgatcacgct ccagggactg
     1081 ccggacgcga agacgcgtta ccggacgacg cgcgacgcac tcggcgcggt cgtcaccgtc
     1141 gaggccgcag gctggaagcg ctcccaggtc gtctcggccg gctactcgtt tctctcctcg
     1201 gggtcgaaaa gccttttctt cgggctcggc gacgcgccgc gtgccgaccg cgtgacgatt
     1261 cgctggccgt cgaggaaggt ttccgagctg cgggacgtcg ccgcgggaaa gatctcgctg
     1321 ggcgagccga tggacggaga gacgctgacg cgctcgcggt gatacccttc actctcgtcg
     1381 aaaaatggag gtttgccgat gatccgacgc ctcacgctga ccctgtcgat ggccgccgct
     1441 ctgactctgc cgctcgccgc ggaagacctg acgatcgtgt cgaaagtgac gaccggaggc
     1501 cgctcgggca cgtcaacgca atacatgacg tcgacgaagt cgcgcacgac cgacggccag
     1561 accgactcga tcatggacta tcccaccggc acgatgacgg tcatcgacca caggaacaag
     1621 acctactggg aggcgacgat ccaggagatg tccgcctaca tggacaagct cgagaaggac
     1681 atgaagggca atccgatgct cgagaacatg ttcggaggtc cggacgacgt ctccgtcgag
     1741 aagggaaagg gctcccgcaa agtcgccggg tacgactgcg acgagtacac catgagaatg
     1801 ggagagtctt ttgtcttcga cctgtgtgcg gcgagaggcc tgcagcctcc accgcagtac
     1861 tacgagggac ggaaactctc gtcagcggcg atggggccga tgggaaagcg cttcgcgaag
     1921 atgttcgaag agatgaagaa gatcaagggg tatcccgtcg ccctcgacat ggacatgaac
     1981 atgggcatgt cgaagatgaa gaccacgtcg gaggccaccg aggtgaagaa gggctcgatc
     2041 ccggcctcga cgttcgagat cccttcgggc tacgagaaga aggcgtcgcc gttcaagcgc
     2101 tgagaaacac tcagagaatc ggtcgctgct cctccttctc ctgcgcgtcg acgaccgcga
     2161 tcgcggccat gttcacgatg tcggcaactt cgaccgcccg ctggaggacg tggaccggct
     2221 tccgcatccc catgaggatg ggtccgatcg cctcggcgcc cgcgagccgc cacacgagct
     2281 tgtatgcggc gttcgcggac tgcagatccg gaaagatgag cacgttcggc cgccgcacgt
     2341 tcgcccacgg gtactcggcc tcgagcagtt ccggcacgac ggcgtagtcc acttgcatct
     2401 cgccgtcgat gggcaggtcc ggacgcgcgg ccttgacgag ctcgactgcc ctcttcacct
     2461 tcaacgcgtt cgcgtgcgtg ttcgacccga agtttgagaa ggagatcatc gcgatccgcg
     2521 gctcgaggtc gaaccggtgc acggcgtcgg cggtcaggag cgcgatctcc gccagttctt
     2581 ccgccgtcgg gtcgatgttg acggtcgtgt ccgcgaagaa aaacgtccgg tccttgaaaa
     2641 tcagaacgta gagccccgag acccgccgca cgcccggagc ggtcccgacg atctgcaagg
     2701 ccggccgaat ggtttccggg tagtgctggg tgagccccga aatgactccg tcggcgtccc
     2761 ccgcctcgac catcagcggc ccgaaggcgt tccgcgcgcc cgcctggtcg agagcatcgg
     2821 tcacggtcac cccgcggcgg cggcggcgct cctcgaggag tcgcgcgtac tccggcagcc
     2881 ggggcgacga ccgggagtgg acgaattcga tcttgtccat cgggagctgg agctctcgcg
     2941 caagcggctc gacctcctcg atccgcgcga gcaggatcgg ccggcagatc ccctcctcga
     3001 ccaggatctt cgcggcgcgg agaatcttcg ggtgctcccc ctccggaaaa acgatccgct
     3061 tcgggtcctg gctcgctttc cggatgacga cgcccatcac ttcgcgcgcc cgcgaaagca
     3121 tcgcctcgag ccgcctcagg tacgccgcca tgtcggtgat cggctttcgc gcgacgcccg
     3181 acgccatcgc ggcctccgcg accgcgggcg cgacccacag gagcacccgg aagtcgaacg
     3241 gcttcggtat caggtaatcg cgcccgaatc gaaactcctg gccgccgtat gccttcgaga
     3301 cggagtccgg cacgtccatc ttcgccagcg cagcgagcgc ctgcgacgcg gccttcttca
     3361 tcggctcgtt gatctcggtc gcgcggacgt cgagcgcgcc gcggaagatg aacgggaagc
     3421 cgaggacgtt gttcacctgg ttcgggtagt cgctgcggcc cgtggccatg atgacgtcgg
     3481 gccgcgccgc cttcgcctcc tcgggcgtga tttccggctc cgggttcgcg agggcgaaga
     3541 cgatcgggtc tctcgccatc cccttcagca tctcgccctg cacgaggccc ttcgccgaga
     3601 ggcccacgag gacgtcggcg ccctggagcg cgtcgatcaa cgtgcgctcc ttcgtctcga
     3661 cggcgaactg cgccttgtag tcgttcatgt cggcggtgcg tcccacgtag atcacgccgg
     3721 acctgtccac catgcgcagg ttctcttttc ggacgccgta agagaggtag aggcgagcgc
     3781 aggcggtcgc ggacgcgccg gcgcccatga agacgacctt cgcgtcctcg atcttctttc
     3841 cggcgatctc gagcgcgttc aggagcgcgg ccgccgaaat gatcgcggtg ccgtgctggt
     3901 cgtcgtggaa gaccgggatc ttcaggcgct tcttgagctc ctcctcgatc acgaaacact
     3961 cgggcgcctt cacgtcctcg aggttgatgc caccgaacgt gggcgagatt cgcgccacgg
     4021 tgtcgatgaa cgcggcgggg tccttcgcgt ccacttcgat gtcgaagacg tcgatgtccg
     4081 cgaacctctt gaagaggacg cccttcccct ccatcaccgg cttgccggcg agcggcccga
     4141 tgtccccgag tccgagcacg gcggtgccgt tcgaaatcac cgcaaccagg ttgcccttcg
     4201 tggtgtattc gtagaccgtc tccgggtcgc gcgcgatctc gaggcacggc tcggcaaccc
     4261 cgggggtgta cgcgagcgag aggtcctcct gcgtgcggca gggcttcgag ggtatgactt
     4321 cgactttccc cttgcggccg cgggagtgat agttgagcgc atcctcgcga gtcatctttc
     4381 tcacgttctg gccaccagtt cctctctcgc cgcaaggact tcgctcgccg cctcggtcgc
     4441 gggcaccgct cggaccttcc gatcgcgtcg cgtaacgacc tccaccttcc cctccttcaa
     4501 cgcacgccct ccgacgttga cgcggatcgg aaacccgacg aggtccgcgt ccttgaactt
     4561 gacgccgggc cgctcgtcgc ggtcgtcata gaagacgtcc atgcctccgc tccggagcgc
     4621 ctcgtagagc gagtcggcct ctttccgaac cgcctcgtcc gcagggttca gcgagacgat
     4681 ggcgatctcg aaaggggcga gtgcgagtgg ccacacgatg ccgtcgtcgt cgtggtgctg
     4741 ttcgacggcg gacgccatcg tacgcccgac gccgattccg taggtgccca tcacgatcgg
     4801 ccgggtctcc cccttctcgt cggtgaactc ggcgcgcatt gccgtcgagt acttcgtgcc
     4861 gagcttgaag atgtgtccga cctcgatccc ccgggcgatc gtcagcggct tcccgcagcg
     4921 cggacaggga tcgccttcac gggccttcga gaacgagaac cactcggacg cggcagcgtc
     4981 gcgctcgagc gagaggccgc ggacgtgcca gtcgcgccgt cccgcgccgg tcacggcatt
     5041 gaccgcgccc cggatcgact cgtccgcgac gacacgaagg tcccgtccgg acagatgatg
     5101 aggaccgacg taaccaacgg gggcaccggt cgcgcgctcg accttctcct ccgacgcgag
     5161 cgtgagattg tcgaccccga gcgcgttctt cagggcgacc tcgttcgcgt cgaggtcgcc
     5221 gcgcaggagc gcgacgacga aaccgttctc cgtctcgtaa acgaggcact tgatcatccg
     5281 cgaggccgtg atgccgagga aatcgaccac ctcgtcgatt ccgccgcggc cgggagtctc
     5341 gacgtcctcg agcgccgccg gtgcctcgct gtcccacggg gacgggcggc ggccggtttc
     5401 cgccttctcg acgttcgcgc cataaccgca ggcagcgcac cgcgcgacgg cgtcctcccc
     5461 cgtgtcggcg agcaccatga actcctccga cgcagtcccg ccgatcgctc cggagtcggc
     5521 tgccacgacc gtgaagtcga ggccgcatct ctcgaagatc cgccggtagg ccgcgtcctg
     5581 agctcggtag gagcggtcga gcccctccat gtcggcgtcg aacgagtagc cgtcgtacat
     5641 gaggaactct cgacctcgca tcagtccgaa ccgcggccgg atctcgtccc ggaacttcgt
     5701 gcggatctga tagagcgtca gcgggagctg ccggtacgac gtcacgcccg cgcgcaccag
     5761 agaggtcacg gcttcctcgg cggtcggcgc caggcagtac gcgccggcct tgcgatcctt
     5821 caagtggaag agaatctcct ccgccgtata gcgcccccag cgcccggctt cctcccagag
     5881 ctcgcggggc tgcaggatcg ggacctcgag ctccagacag ccggcgcggt ccatttcctc
     5941 ccgcacgatc gtttcggtct tccggtgcga gcggagtccg agcggcgtca acgcgtagat
     6001 cccggcggcg atgcggcgca tcatgcccgc ccgggtcagc aggacgtggg agatcgcctc
     6061 ggcatcgccc gggacctcgc ggacggtctg gagaaagtat ctcgaccagc gcatgtgtgg
     6121 cgccgaagag tatcagcggg ggccgttatt tcggactctg ggctttgggc ttcggactct
     6181 ctttcttctt cggacccgcc ttctcgagca gccgcaggtt gcttcgaacc tgctgcatca
     6241 tgcggtcctc gagcttgccc ccgctcgcca cgttcttcgc cttgtggaat gcgcccttcg
     6301 cgtcttcgag ccgccccgcg gcgaggtagg cgagtcccag ctgatgaaac gtcgtcggcg
     6361 tggccgcgcg ctcgctcgag cggcggaggc agtcgatggc ccggtcgagc tcgtggcgct
     6421 tgtagtggac ccagccgagc gcggcgagcg gatagggctt cagctcttcc ggcgcggccg
     6481 cgagagcgcg ccccgcgtag tcgagcgccg agtcgaggtc ctctcccgat tcggcgaggt
     6541 tggtcgcgag ctcgtagtag ccgaccgcgc gcgccgtcga ggattgatga ccgtcgagaa
     6601 agtcgcgcac gacctgtgcc gcctcggcgg gccggccttc ggcgcggaga gcctccgaga
     6661 gaatcgagca agccgccgcg gcgaccacct cctccggctc ccccgagagc acttcgcggc
     6721 acgaggcgat cgcctcggtc cacagcccca gcgacgccga gaggagcgcg taggagatac
     6781 ggatcgtcga gttttccggc tcggcggtca gggcctgccg atagagctcg tgcgcccgcc
     6841 gcagcgaacc ccgcgcgacg ctctcttccg cccggcgata cgcctccgct ccgggccctt
     6901 cgacgtccgg gagcggatag aacttgcgcc gttcgaggat ccggaccgct tcctggtact
     6961 cgagcttccg cgggttcttt tcgagagctt cgtgaaaaca ctcgagggcg cgcttcagcc
     7021 ggttcttttc gaggtggcag aggccgagct gaaaaaccgc ctcctccgag tccggctgga
     7081 gcttcaacgc ttcttcgagg tgccggatcg cgcggttgac gtcccccttc tcgcgcaaca
     7141 cggtcccgag caggacgtgc gccgaaggct gcggagcgat gtcgaccgcc ttcgcgagct
     7201 ccctctccgc ctcgttccag cgcgacgcct gggcgtagag cgcgccgagt gcgaaatgag
     7261 gcagaaacgc atcggaacgc cgctcgatcg cacgccggtg gaaggactcg gcttcctcgg
     7321 cattgcccgc ctccgacgca acgatgcccg agtaaacgag cgactcgaag tggttcggct
     7381 ggaccgacag gatctttttc aggtagcccg acgcccgctc catgtcgccg gccgagaaaa
     7441 aagtctccgc gaggtagaag ccgagctcgg tgttcgatcg atcgaccttg tagagatcct
     7501 cgagcgcgcg gatgccgcga tcggcatcga gcgcgagcag ggcaaactcc gcctcgcgga
     7561 ctcttttcgt gaagaggtcg ctgttcgctc ccgagaagcg gccgatgatt cggtcccgcc
     7621 gctcgaggaa ccggtccttc ttctcgaccg cctgcatgcg ctcgtcgagc ttcgtctccc
     7681 agagatcacg gacctcctcc gcccggacga cgccccgacg ctcgagcatc tcctgcagag
     7741 cgaggacccc cgtgtgcgcc acgaaaaggt tcttttcgag gttgccgaga gattcgaaga
     7801 gcgacttgag cgcgtcgccc gtccgcttga cgacctcctc gagcgtcgag atccgttcga
     7861 gcaggtgctc gtcgaagggg atctcctcct ggacctcccg accctcctcg accaccccga
     7921 ccccggagag gacgaggagt ttcgactgac agcggacgca gaactcctcg tcgtcggggt
     7981 tgcgggaccc gcatacctgg cagaacatgc ttacgggcgc tgccgggccg gctcggacga
     8041 gtattcgtaa aagcccttgc cggtcttccg gccgagccat ccggcgtcca ccatcttcac
     8101 gaggaggggg ctcgggcggt accgcgggtc gccgaagccc tcctgaagga cacgcagaat
     8161 cgagaggcac gtgtcgaggc cgatcaagtc ggcgagcgcg agcggcccca tcgggtggtt
     8221 cgcgccgagt ttcatgatcg agtcgatcgc ctcccgggtc ccgacgccct cgcgcagggc
     8281 gtcgatggcc tcgttgatca tcggcagcag cacgcggttc gccacgaagc ccggcgagtc
     8341 gttgcaggag acgggcgtct tccccatctc ccgagccacc ctctcgaccg cggcggcggt
     8401 ctcgtcggag gtcgcgatcc cacggatcat ctcgatgagc gccatgaccg ggacggggtt
     8461 catgaagtgc atcccgatga accgttcggg acgccgcgtc gcggcggcaa gccgcgtgat
     8521 cgatatcgaa gacgtgttgg acgcgaggat cgtcgaaggg ccgcagaccg cgtcgagccg
     8581 ctggaagagc tcccgcttcg ccccttcgtt ctcgacgatc gcctcgatga cgaggctcgc
     8641 cgcggcgagc cgatcgaggt tcgtggttgt cgcaatccgg ccgagggcgg ccgacctgac
     8701 ctccgccgac ctttttccct tcgcgacttc ccggtcgagg tttttctcga tcgtggcgcg
     8761 ggctttttcg agaaaacgct cttcgacgtc ggtgaggatg acttcaaaac cggacgcggc
     8821 cgccacgtgg gcgatcccgt ttcccatctg cccggctccg acgacaccga ttgttcgggg
     8881 ggacatccgg ggccgactct attgccccgg cgtcaccgga atcaaatcgc gcggagagcc
     8941 acgcggacgt ggggagcgcc gaaccgggac acgaacgcga cgccgaccgc ggcgaccgag
     9001 gcggcgctcc agacgattgg tcccgcaacg ggaagaagag acaggccccc gagcaccgcg
     9061 aagccgacgg cgagcgccga cggccgccgc cccggcgaga agctcgcggt cagtttctgc
     9121 ccgagcagga gaaagagcgc gcccatcccg aaaaccttcg cggcgatcgc ggccgaggcg
     9181 agagcgaggg ccacgggaac ggcgactgcg gaaggcagaa gcgcaagagc ggcggcggca
     9241 gcgagaaaga tcgtgagaac cccgagaacg cctgccgtga gcgctcccgt ccagttcgac
     9301 tccgcgcgat cggccgcgcg tgcgagcgga gaaccgaaga agtagaggag cgcgaccgcg
     9361 accgcgagcc acgccgagag cgcgaggagc cgaaggcccc agacgaccgg cgccagcgcg
     9421 ccgggctccc acggtgccct cttcatctcg gcgaggtaga gccggaggag ggtgccgggc
     9481 gtcgacacgg tgccgccgac cggcagcgcg cggcccggca cgccgcggat cgttcctccg
     9541 aagacgacga gattcccggc gaccgttccc gccggaccga aggagacgtc gccgccccac
     9601 acgatcacgt cgccggcgac cgcgctctcg acgcgcacgt cggcggcgac cgcgacgatc
     9661 cggcccggga ccttctcggt gacgacgaca tcgcggccga ccgaggagat cccggacggg
     9721 gaggcggcgg cgggcgatgc gagaagcagt gcgagtgcgg cgaccgccgc gatcacgaac
     9781 ggcggctccg ggcgcgggcg aatacgcgtg tgagaccgaa cgcggacggg atgaggagaa
     9841 gcagagaggc gaaaccgagc gaacgctccc gtgcgagagc gtcggcgagc gcctcgaggg
     9901 cggacggcgc cagacgcgcg agatcgcttc cccacctcgg aagcgatcgc aggaagccaa
     9961 ggaccggcgc cagcgccgcg gcggagagac ccgcctgctc cccgcccgag agcgacggcc
    10021 cgacgaggag cccgagaccc ccgaccgcga gagcgaccgc caggaggaca ggccactgcc
    10081 acagggcgta agtccggcgc tcccgggcag agaaggcccg gaggcggctc accctccccg
    10141 ccaggtcggg gagaggcgaa tcggcctgcg cgagcccctc cagggcccgc gtcagggcct
    10201 ttgcgcgctc gacggacgcc ccgcaggccg ggcaggaggc cgcatgagcc gccgcatccg
    10261 gcgcaagcac gggttcggcg cccgaggcga gcgcctcggc gtcgatcggg tccagcggac
    10321 gggcacaagg gttcacgaca actctccagc caaccgttcc ttcaatacga ctctggcacg
    10381 aaagagtttg ttcttgaccg tcccgagagg catgttcttg acccgggcga tctcctcgta
    10441 ggacaggccg ccaaagtggc gcagcgtgat cagctcccga aactcgggcg gcagatcggc
    10501 gattgcccgg gaaatggcct cgccccgctg caggttgcgg agctgcccgt acgggtcgag
    10561 cccggcgctc tcgtgctcga gggaggagac gcccgaacgg ctgtcgggat ccgggatctc
    10621 gagcggcacg gtacgcggac ggcgcttgcg cagatggtcg atcgctgcgt tccctgcgat
    10681 ccggaagagc caggtcgaga acttgaactc ggggttgtag cggtcgagag cctggaagac
    10741 cttcaggaag atctcctgcg cgagatcgaa cgcgtcatcg cggctgccga ccatccgtgt
    10801 gacgtgcgcg acgacgcggg cctgatagcg ccggacgagg acctcgaaaa gcggttcttc
    10861 cccggcgaga atccgctgga cgagaacgcc gtcgttgtcg agttccattg cggcgggctc
    10921 ggatcccggg acggggtcgg atcccccggt ccggacagtc atgccgagaa cgtaagtcgg
    10981 gaagaacggc tgggatacaa tccgcgccca ttttaccgaa ggccggcggc ccgcctgccg
    11041 aggagtgaag ccgtggatca agtcgcggaa caggagctcc ccaccctcac gacgaccact
    11101 ctcccgaccg gcgtcgacag cggaagcccc ttcgacgaaa tgatgtcgcg cttcgacgtc
    11161 gcggcgcaga agctcggcct cgaccccggc ctttacaagg tcctccggga gccggtccgc
    11221 gagaccaagg tctcgatccc ggtcgtcatg gacgacggcc acatcgaggt cttcatcggt
    11281 taccgcgtcc tccacaacat cgcgcgagga cccggaaagg ggggcatccg cttcgacaag
    11341 aacgtcaccc tggacgaagt tcgcgcgctg gcggcctgga tgacgtggaa gtgcgcggtc
    11401 gtcaacattc cgttcggcgg ggcgaagggg ggcgtcatct gcgatccggc gacgctctcc
    11461 cggggcgagc tcgagcgcat cacgcgccgg tacacggcgg agctcatgga ccagttcggt
    11521 ccggagaagg acgttccggc gccggacatg ggcacgaacc cccagacgat ggcgtgggtc
    11581 atggatacct actccatgca cgcgcgccac acggtgactt cggtcgtgac ggggaagccg
    11641 ctctcgctcg gcggctcgcg cggccgcgtc gaggcgacgg gccgggggct catgctcatc
    11701 gcgcgcgagg cggcgcctct gatcggcctg acgctgtccg ggagccgggt ggtcgttcag
    11761 gggttcggca acgtcgggtc gatcgcggcg cgcatgtgtc acgaggcggg cgcgagagtg
    11821 ctcgcggtct ccgacatcaa cggcggaatc gtcaatcccg cggggctcga catgccggcc
    11881 gtgctcgcgc actacgagag ggcgcggacg ttcgaaggct tccgggacgc ccggaagatc
    11941 tccaatgcgg agctgctcga gctcgactgc gacatcctga ttccagcggc caacgagaac
    12001 cagatccacg gacgcaacgc gcccaacatc aaggcgaaga tcatcgtcga gggcgcaaac
    12061 ggcccgacga cccagcaggc cgacgaaatc ctgcatcaga agggcgtact cgtggtcccg
    12121 gacatcctcg cgaacgccgg cggcgtcact gtctcctact tcgagtgggt ccaggaccgc
    12181 gccggctttt tctggcggga gcaggaggtc aacgagcggc tcgaagacat cctggtccag
    12241 tcgttccacg acgtccgctc gatggccgag aagtacaacg tcagctaccg catcgcggct
    12301 tacatgctgg ggatctcgcg ggtcgcgcag gacacgatgg tccgcggcct gtacgcgtga
    12361 cgacccgctc ccggtaacga cctttcccgg cgcctacctc ccgcggatca gcaggatcgc
    12421 cgcgcccgcg agcgccagac cgagcatcgc catcatcggg aaggacagcg tcgggacggc
    12481 agcagcgggt cccgccatcg cggtcgctgt ggccgtggcc tgcgcgagtg tcggagtcgg
    12541 ggtgggcgtg gcggcgaccg tcgtggtggt cggcgttggt gtcgggaggc cgaccccgca
    12601 gggagtcacc gtggcggacg cgtacaggcc gtactgaagg tgatcgacct cgagtccgcc
    12661 gcctgtgttc gagatcttga tcgccgatat gcccccgggg aaggtgacgc cgaagaaccg
    12721 gtcttccgcg gtctcccccg agttcgacgc gtcgccgatg gcgaccggcc cgatcacacc
    12781 gagagaagcg ccgatggaat caaatgcctc gaacgtcgcc gttccgccgc cgtcggtcca
    12841 gacgattccg acttccgtcg gaaacgctcc cagggtcgtc tggttgaaat tgaacgtgat
    12901 cccggtcgcc ccgtcgcccg tgaagagcga gtggccgccg agtcctgagc cgtcgatcga
    12961 gccgtcatcc gcgtccaccg agtcgatcaa cccctcgaac ccgggcggga tgatcgtccc
    13021 ggtggaaggc gtcacgccgg gcaccgtcag attgccgcac tcgaaggttt ccagatagac
    13081 gtggcccgcg ttgatgtccg cgatgaaagg gctgtccgct ttcgacaggt agggagtcgg
    13141 gcccagaaag gtcgggcttc cccctgcaaa ggcgcggctc gaagcgagaa ggagcagcac
    13201 cccgaaagag aggacggcaa gccgggggcg gaaccggagg gcgcaaacgg ccatctttgg
    13261 ttc
//
LOCUS       DASWAV010000181         3434 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U2665
            k141_370352, whole genome shotgun sequence.
ACCESSION   DASWAV010000181 DASWAV010000000
VERSION     DASWAV010000181.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960316
            Sequence Read Archive: SRR10260007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 3434)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3434)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:47:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,929
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3434
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_370352"
                     /isolate="SMAG_U2665"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.36444 N 132.31889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..539)
                     /locus_tag="VF958_03595"
     CDS             complement(<1..539)
                     /locus_tag="VF958_03595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9148223.1"
                     /translation="MASLWLPILLSAVIVFVASSLIHMVLRYHRNDFVRLPSEDAVMD
                     VLRSASPGDYFFPWAGGPSAMKDPAFVEKRKRGPVGVLTLLKPGPPTMGPSLARWFVH
                     CIVVGIFAAYIAGRALGPGAPYLSVFRFAGCTAFIAYALAYWQNSIWYGRKWSTTFKV
                     TIDGLIYGLLTGGTFGWLWP"
     gene            complement(577..1377)
                     /locus_tag="VF958_03600"
     CDS             complement(577..1377)
                     /locus_tag="VF958_03600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014618138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HEX9148224.1"
                     /translation="MSEFSSLRVEREGPVARVTLARSEVRNAFNEVMIEELTQVFLSF
                     GDDRGTRVVLLEGEGPIFSAGADIEWMRRAGNWSKLENEADAERMARMLRAIDRCLLP
                     VVARVQGAAIGGGVGLVAASDIAIAAEDAVFSLAEVKLGILPSVVSPHVLRAIGARQA
                     RRYFLTGDRFSAAEARRIGLVHEVVPAPELAAACDRTIGALLTSGPEAVRFAKRLIEE
                     VAGKSPDEAMSVTVRTIAERRASEEAKEGLTAFLEKRPPSWAPKKPSQ"
     gene            complement(1374..2141)
                     /locus_tag="VF958_03605"
     CDS             complement(1374..2141)
                     /locus_tag="VF958_03605"
                     /inference="COORDINATES: protein motif:HMM:NF015147.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LEA type 2 family protein"
                     /protein_id="HEX9148225.1"
                     /translation="MIAGLLVGLVLAVLGRPVEIDIRPGPTETFSATLVGPAPGVGAG
                     PFAGRISVNGSSAELPVETRAEISGSGRLRLALTLRYSDVPRDWANRFRADTFEYRVS
                     GRVAGSSPVEWIGTARWDGVGVESERETVGRFVRLVTLEVTDVSLFSSDARAVVSVRN
                     PFSFPLTIASSRYRVTADGRLVGSGSTRGFLLRPRQVSTVLLPLEVEHGALIAAAGGA
                     VLSGEGVPARIAGTLDVRLPGGDVAVPIDLAGQLSFR"
     gene            complement(2144..>3434)
                     /locus_tag="VF958_03610"
     CDS             complement(2144..>3434)
                     /locus_tag="VF958_03610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014435087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="carboxyl transferase domain-containing protein"
                     /protein_id="HEX9148226.1"
                     /translation="NDATVKGGTYFPITVKKHLRAQEIALENRLPCVYLVDSGGAFLP
                     LQADVFPDREHFGRIFYNQARMSAARIPQVAAVMGMCTAGGAYVPAMSDEAVIVKGTG
                     TIFLAGPPLVRAATGEVVSAEDLGGADVHTRVSGVADHFAEDDAHAIEILRSIVENLG
                     GSKSLPADRAEPEEPLYDAGEIYGVLPRDVRKSLDVREVIARLVDGSRFHEFKARYGA
                     TLVCGFARILGFRVGIVANNGILFSESALKATHFIQMCARRRTPLLFLQNITGFMVGK
                     DYERGGIAKDGAKMVHAVANAAVPKFTVIIGGSFGAGNYGMCGRAYGPRFLWMWPNAR
                     ISVMGGSQAASVLSTVKQDQLARAGKPPMTDTEVADFERPTREKYEVEGNPYYSTARL
                     WDDGVLDPAETRTALSLAISAAYNAPIPETEFGIFRM"
BASE COUNT          597 a         1199 c         1129 g          509 t
ORIGIN      
        1 ggccagagcc agccgaacgt tccacccgtc agcagaccgt agatcagccc atcgatggtc
       61 accttgaacg tcgtcgacca cttccggccg taccagatcg agttctgcca gtaggcgagt
      121 gcataggcga tgaacgccgt acaccccgcg aaacggaaga ccgagagata gggagcgccg
      181 ggcccgagcg cgcggcccgc gatgtacgcc gcgaagattc cgacgacgat gcagtgcacg
      241 aaccagcgcg cgagggacgg tcccatcgtc ggcgggccgg gcttcaggag cgtgagcact
      301 ccgaccggtc ccctcttcct cttctcgacg aacgccgggt ccttcatcgc cgaaggccct
      361 cccgcccacg gaaagaagta atcgccgggg gaagcggatc gcaggacgtc catgaccgcg
      421 tcctcggacg gcaggcgaac gaagtcgttt cggtgataac gcaagaccat gtggatgagc
      481 gagctcgcca cgaagacgat gactgccgac agcaggatcg gaagccacaa tgacgccacg
      541 ggaaccatcc ttcacctcct cgactgctaa cgcggactat tgcgacggct tcttcggcgc
      601 ccaagagggc ggtcgcttct ccaggaacgc ggtcagacct tctttcgctt cctccgatgc
      661 gcggcgctcg gcgatcgttc gaacggtgac cgacatcgcc tcgtccgggc tcttgcccgc
      721 cacctcttcg atcaaccgtt tcgcgaagcg aactgcctcg gggcccgagg tcaggagcgc
      781 accaatcgtt cgatcgcacg cggcggcgag ctcgggcgcc ggtacgacct cgtgtacgag
      841 cccgatccgt cgggcttcgg ccgcgctgaa gcggtctccg gtcaggaagt agcgccgtgc
      901 ctggcgagca ccgatcgcgc gcaggacgtg aggcgaaacg acggagggaa gaattcccag
      961 cttgacttcc gccagcgaga acacggcgtc ttccgccgcg atcgcgatgt cggaggccgc
     1021 gacgaggccg actcctccgc cgattgcggc gccctgaact ctcgcgacga cggggagcag
     1081 gcagcggtcg atggctcgaa gcatccgggc catccgttcg gcgtccgcct cgttctcgag
     1141 cttggaccag tttcccgcgc gacgcatcca ctcgatgtcc gcccccgcag agaagatcgg
     1201 cccctcccct tccagcagga ccacgcgggt gccgcggtcg tcgccgaacg acaggaagac
     1261 ctgcgtcaat tcttcgatca tcacctcgtt gaacgcgttg cggacctcgg accgggcgag
     1321 agtcacccgg gccaccgggc cttcgcgctc gactcggagg gaagagaact cgctcatcgg
     1381 aacgagagct ggccggcgag gtcgatgggg acggcgacgt cgccgcccgg cagccgcacg
     1441 tcgagcgtgc ctgcaatccg ggccggcacg ccttcgcccg acagcaccgc gccgccagcc
     1501 gcggcaatga gcgcgccgtg ctcgacttcg agcgggagca ggaccgtgct cacctgtcgt
     1561 gggcggagga ggaatccgcg ggtcgacccg cttccgacca gccggccgtc ggcagtgacg
     1621 cggtagcgcg aggacgcgat cgtgagcgga aaggagaagg gattgcggac ggagaccacg
     1681 gcgcgtgcgt cgctcgaaaa gagcgacacg tcggtcacct cgagggtcac gaggcgcacg
     1741 aagcgcccga cggtttcgcg ttcgctctcc acgccgacgc cgtcccacct cgccgtgccg
     1801 atccactcga cagggctcga cccggcgacg cggccgctca cccgatactc gaaagtgtcc
     1861 gcccgaaagc ggttcgccca gtcccgcggg acgtcggaat agcgcagcgt cagcgcaagg
     1921 cgcaggcgcc cgctgcccga gatctcggcg cgcgtttcca ccggcagctc ggcagaggag
     1981 ccgttgaccg agatccggcc ggcgaacgga cccgcaccga cgcccggagc tggtcccacg
     2041 agcgtcgccg aaaaggtctc ggttggaccg ggccggatgt cgatctcgac cggccggccg
     2101 agcaccgcga gcacgagtcc gacgagcagt ccagcgatca tggtcacatc cggaagatgc
     2161 cgaactccgt ctccggaatc ggcgcgttgt aggcggcgga gatcgcgagc gagagcgccg
     2221 tccgcgtctc ggcggggtcg aggaccccgt cgtcccagag ccgggccgtc gaatagtagg
     2281 ggttgccctc cacctcgtac ttttcgcggg tgggccgctc gaagtcggca acctccgtgt
     2341 cggtcatcgg cggctttccc gcgcgcgcga gctggtcctg tttgaccgtc gagagcacgg
     2401 acgccgcctg tgagccgccc atcaccgaga tccgggcgtt cggccacatc cagaggaagc
     2461 gcggcccgta cgcccgcccg cacatcccgt agttcccggc gccgaacgag cctccgatga
     2521 tgaccgtgaa cttcgggacg gcggcattcg ctacggcgtg caccattttc gcgccgtcct
     2581 tcgcgatgcc cccccgctcg tagtcctttc cgaccatgaa gccggtgatg ttctgcagga
     2641 agaggagcgg cgtcctgcgg cgggcgcaca tctggatgaa gtgcgtcgcc ttgagcgccg
     2701 actccgagaa gaggattccg ttgttggcga cgatgccgac ccgaaatccc aaaatccgcg
     2761 cgaacccgca gacgagggtc gcaccgtagc gcgccttgaa ctcgtggaag cgcgatccgt
     2821 ccacgagccg ggcgatgacc tcgcgcacgt cgagcgactt ccgcacatcg cgaggcagga
     2881 ccccgtagat ctcgccggcg tcgtagagcg gctcctccgg ctccgcacgg tcggccggga
     2941 ggctcttcga tccgcccagg ttctcgacga tcgaacgcag gatctcgatc gcgtgcgcgt
     3001 cgtcctctgc gaagtggtcg gcgacgcccg atacccgcgt gtggacgtcg gcgcccccga
     3061 ggtcctccgc cgacaccacc tcccctgtcg cggcgcgcac gagcggcggc cccgcgagaa
     3121 agatcgtgcc ggttcccttc acgatcaccg cctcgtccga catcgccgga acgtaggcac
     3181 cgcccgccgt gcacatcccc atcacggccg cgacctgggg gatgcgggcg gccgacatcc
     3241 gcgcctggtt gtaaaagatc cgcccgaagt gctcgcggtc ggggaagaca tccgcctgga
     3301 gcggcaggaa cgcgcccccg gaatcaacga ggtacacgca cggcagacga ttctcgagcg
     3361 cgatttcctg cgcgcgcagg tgtttcttga cggtgatcgg aaagtacgtc ccgcccttca
     3421 cggtggcgtc gttg
//
LOCUS       DASWAV010000182         6113 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U2665
            k141_289750, whole genome shotgun sequence.
ACCESSION   DASWAV010000182 DASWAV010000000
VERSION     DASWAV010000182.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960316
            Sequence Read Archive: SRR10260007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 6113)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6113)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:47:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,929
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6113
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_289750"
                     /isolate="SMAG_U2665"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.36444 N 132.31889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..834)
                     /locus_tag="VF958_03615"
     CDS             complement(<1..834)
                     /locus_tag="VF958_03615"
                     /inference="COORDINATES: protein motif:HMM:NF014081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX9148227.1"
                     /translation="MRKLFAFAAAFALEAGLLAGLQQPSPPRPTPPSAPPGRGSTPSP
                     GGPASATPLVALKAARLFDGRSDRTIENAVVIVEGTRIRDVGSGLAIPPEATALDLGD
                     ATLIAGFIDSHTHLTDEASDNWLAGFYEGLRRPVAEKAIIATGYARKTLEAGFTTVRD
                     VGSSDSIDVGLRNAINRGLIPGPRMLVARDAIGATGGHCDQTGFPEGTFGQEPGIEKG
                     IIHGAAEARQAVRLDIKYGADVIKTCASGGVLSLGDDVGSQQLSDEELTALVDEAHRL
                     GK"
     gene            complement(886..1392)
                     /locus_tag="VF958_03620"
     CDS             complement(886..1392)
                     /locus_tag="VF958_03620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016860555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rab family GTPase"
                     /protein_id="HEX9148228.1"
                     /translation="MIQKKVCMLGTYAVGKTSLVSRFVRSLFAEKYHSTVGVKVDKKV
                     VAVGEEQMNLLLWDLYGEDDFQKLRTSYLRGSSGYILVVDGTRHETLEKAVILQSRAR
                     EAVGAAVPFVLALNKADLADKWEVEDSAVEELVKQGWPTFKTSAKTGAGVEQMFLTLS
                     RRMAGLST"
     gene            complement(1389..3188)
                     /locus_tag="VF958_03625"
     CDS             complement(1389..3188)
                     /locus_tag="VF958_03625"
                     /inference="COORDINATES: protein motif:HMM:NF012894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HEX9148229.1"
                     /translation="MDAFVGKGNGADETAPAAAPDPIEELRRLLLVAEHSQVAKIQER
                     LDDPEIHAEDVSRVLPEAIALRATKDRDLTRAMLPTVEEAILSSVRKDPHVLVDALFP
                     VMGPAIRKAISSALASMIESLNETLEKSFSVQGLKWRWEAWRTGKPLPEIILLRTLVY
                     RVEQVFLIHRKTGLLLQHVSATSGGVQDADMISGMLTAIRDFVHDSFGGNREDSLDSF
                     RVGEFTVRIEQGPEAVLAAVIRGNAPPELRSTLTRAIEAIHLEQGEALANFEGDATPF
                     ERSRPHLEECLKTQQRSRAERGAGARSRRAIAFLRGALAVLLIGIAVWSFFAIRRSRR
                     WNAYLERLAGEPGIAVIGSGRRGGRYFVRGLRDPLAVDPVAMLAESKLARESVSSDWK
                     PYQALVPEFIAARARDVLEPPRTVTLTAGDGKVMATGSAPHRWIEEARARSRAFAGLA
                     RYDDARLADDDGRELEALSKAIEGRPISFDLASAELRPEESDTLATLSRDLSRLAQLA
                     AAVGRNVRVEVIGRGDSSGTPEANLAISRLRAERVARALSSTRESGVTFAARGVGSSE
                     PLRPEIDEKDRELNRSVTLRVLVEGGPGRSVTR"
     gene            complement(3208..3825)
                     /locus_tag="VF958_03630"
     CDS             complement(3208..3825)
                     /locus_tag="VF958_03630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9148230.1"
                     /translation="MSTPGEEKRGAPGQEPKAGNGSATGKGAAQEGVGGGANLEKIRD
                     ILFGAQVHDFEKRFSRLEERLLKETSDARAETKKRFDSLESFIKKEVESLGDRLKAEQ
                     EERSGAVKDVSRELRDTARGLEKRLAQLDEQTTKSQRELRQQVLDLSKNLTDEIRLRN
                     RETTTALSKEIAELRNAKADRATLAGLFTDLAVRLTTDLKAPAKS"
     gene            complement(3888..4298)
                     /locus_tag="VF958_03635"
     CDS             complement(3888..4298)
                     /locus_tag="VF958_03635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9148231.1"
                     /translation="MSEKTIAADLIAALEFAVFEREEGGRFRLLGAPPAWFASLSPTG
                     SISFPFLDNFLVDAEAFWTNAAGGRLKSGIWIESDALGHETPLEASAVCVGPRRILLI
                     ENQTLVHDEKQAVLQRARNAKLRERGRTGKGRPP"
     gene            complement(4380..5735)
                     /locus_tag="VF958_03640"
     CDS             complement(4380..5735)
                     /locus_tag="VF958_03640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019304999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HEX9148232.1"
                     /translation="MSPEIARLDPKTGLLRAVSRWQIVALSVNDVIGSGVYLILPVAA
                     AQLLGPASVWAILAAGFGVLLLVLCFAEASSLFDRQGGAIVYTRAAFGDFVGFEVGWM
                     TWIARIASIAGLSVFFARAVGYLWPGARSGLGQYATIVLPLLVLTWINVLGVKSGART
                     AVVLAWGKVLPLVLFVAVGIFFVDWGRVFPVPTPERANFTKAALLVLFAYAGFENTPA
                     PAGEFVNPKRDVPFALIVQIVIVTAIYTTVQLVAIGTVPNLGSSPTPLADAAAMMMGP
                     VGGLVLTLGAVLSVLGTNNNTVLAGPRYLYALAETGRLPAVFAKVHPRYRTPHVAIIT
                     QTAVALMLILVDWGINALLPAKSFLGLAERLAMLSAIARLATYIGTAAAVPVLRRKLP
                     ATERTIRLPGGPLIPLAALAICLLFLSAAEGKNWITGGIALAVGAVIYLLRRGSPPLS
                     A"
     gene            complement(5855..>6113)
                     /locus_tag="VF958_03645"
     CDS             complement(5855..>6113)
                     /locus_tag="VF958_03645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9148233.1"
                     /translation="SAVSRHQYGDASDVYPDDDRDGRLDDLNRDGRVDLKDARILASA
                     AEVVERKNPQLIGGIGIYPATSSHGPFVHIDARGTRARWNG"
BASE COUNT         1000 a         2038 c         2043 g         1032 t
ORIGIN      
        1 ctttccgaga cggtgtgctt catcgacgag cgcggtgagc tcttcgtcgg agagctgctg
       61 cgatccgacg tcgtccccga gcgacaggac ccctccggac gcgcatgtct tgatcacgtc
      121 ggcgccgtac ttgatgtcga gccggaccgc ctgacgggcc tccgcggcgc cgtgaatgat
      181 gcctttctcg atgccgggct cctggccgaa cgtcccttcg ggaaagcccg tctggtcgca
      241 gtggcctccg gtcgcgccga tcgcgtcgcg cgcgaccagc atgcgcggcc cggggatgag
      301 gccgcggttg attgcgttgc gcagtccgac gtcgatcgaa tcgctcgacc cgacgtctcg
      361 caccgtcgtg aatccggcct cgagggtctt ccgggcgtaa cccgtggcga tgattgcctt
      421 ctcggccacc ggccggcgca gaccttcgta gaagccagcg agccagttgt cggaggcctc
      481 gtcggtcaga tgagtgtgcg aatcgatgaa gcccgcaatg agcgtggcat cgccgagatc
      541 gagggcggtc gcctccggcg ggatcgcgag gccgcttccg acgtctcgga tgcgggtgcc
      601 ttcgacgatc acgaccgcgt tctcgatcgt gcgatccgac cgtccgtcga agaggcgcgc
      661 cgccttgagg gcgacgagcg gcgtcgcgga tgccggcccg cctggcgagg gcgtcgagcc
      721 gcggcccggg ggggccgaag gcggggtggg gcggggtggc gacggctgtt gaagccccgc
      781 gaggagaccg gcctcgaggg caaacgctgc ggcgaaggcg aaaagcttcc tcatcttcgt
      841 atttcctccc tggttcgtcg tcgttttctt gtcgcgccgg gaagctcagg tcgacagacc
      901 ggccatcctc cgggaaagcg tcagaaacat ttgctcgact ccggcgccgg tcttcgcgct
      961 cgtcttgaag gtcggccacc cctgtttgac gagctcttcg accgcggagt cttcgacctc
     1021 ccacttgtcg gcgaggtcgg ctttgttgag cgcgagaacg aacggaacgg cggcgcccac
     1081 ggcttcccgc gcccggctct ggaggatgac cgccttttcg agcgtttcgt ggcgtgtccc
     1141 gtcgaccacg aggatgtagc cggaagagcc gcggagataa gaagtccgga gcttctggaa
     1201 gtcgtcttcc ccgtagaggt cccagagaag gaggttcatc tgctcctctc cgaccgcgac
     1261 gaccttcttg tcgactttta cgccgacggt cgagtggtac ttctcggcga acagacttcg
     1321 gacgaagcgc gacacgaggc tcgtctttcc cacggcgtac gtgccgagca tgcagacttt
     1381 cttctggatc atcgcgtgac ggacctcccc ggaccgcctt cgacgagaac gcgcagagtg
     1441 acgcttcggt tgagctcccg atccttctcg tcgatctcgg gccggagcgg ttccgatgat
     1501 cccactcccc gggcggcaaa cgtgacgccg ctctcccgcg tcgaggagag cgcgcgggcg
     1561 acccgctccg cccgcaggcg gctgattgcc aggttggcct caggcgtccc gctgctgtcg
     1621 ccgcgcccga tcacttcgac tcgaacgttt ctgccgaccg cagcggcgag ttgggccagg
     1681 cgggaaagat cgcgcgacag agtcgcgaga gtgtcggact cttccggtcg gagctccgcc
     1741 gaagcgagat cgaacgaaat cggtcggccc tcgatcgctt tggagagcgc ttcgagctcg
     1801 cgcccgtcgt cgtcggccag ccgggcatcg tcgtaccgcg cgaggccggc gaatgcgcgg
     1861 gatcgcgcgc gggcttcctc gatccagcgg tgcggcgcgg accccgttgc catcactttc
     1921 ccgtcgcccg cggtgagcgt cacggttcgg ggcggctcga gaacgtcgcg cgcgcgagcg
     1981 gcgatgaact ccgggacgag cgcctggtat ggcttccagt cgctcgacac gctttcgcgc
     2041 gcgagcttcg actccgcgag catcgcgacg ggatcgacgg cgagagggtc gcggaggccg
     2101 cgaacgaagt acctgccgcc gcgccttccg ctcccgatga ccgcgatccc gggttcaccc
     2161 gcgagccgct cgaggtatgc gttccagcgc cggctgcggc ggatggcgaa aaacgaccag
     2221 acggcaatac caatcaagag gacggcgaga gccccccgga ggaacgcaat cgcccggcgg
     2281 ctccgggcgc ccgcgcccct ctcggcccgg ctccgctgct gcgtcttcag gcattcctcc
     2341 aggtgcggac ggcttctctc gaagggtgtc gcatcccctt cgaagtttgc gagcgcctcg
     2401 ccctgctcga ggtggatggc ctcgatcgcc cgggtgagcg tcgaacgcag ctccggcggc
     2461 gcgttcccgc gaatgaccgc cgcgagcacc gcctccgggc cctgctcgat ccggacggtg
     2521 aattccccca cccggaaact gtcgagcgaa tcctcccggt tcccgccgaa ggagtcgtgg
     2581 acgaagtcgc ggatcgccgt gagcatgccg gagatcatgt cggcatcctg cacgcccccc
     2641 gacgtcgccg aaacgtgctg cagcagcagg cccgtcttgc ggtgaatcag aaagacctgc
     2701 tcgacgcggt agacgagcgt gcggagcagg atgatctccg gcagcggctt acctgtccgc
     2761 caggcctccc atctccactt gagcccctgg accgagaaac tcttctcgag cgtctcgttc
     2821 agcgactcga tcattgaagc gagcgcgctc gagatggctt tgcgtatcgc cggtcccatg
     2881 acgggaaaga gcgcgtcgac taagacatgg gggtccttgc gcacggacga gagaatggct
     2941 tcctccaccg tcggaagcat ggcgcgcgtc agatcccggt ccttcgtcgc acgaagcgcg
     3001 atcgcctccg ggaggacccg gctcacgtct tcggcgtgga tctccgggtc gtccagtcgc
     3061 tcctggatct tcgcgacctg ggagtgctcc gcgacgagga gcaggcgacg cagctcctcg
     3121 atcggatccg gagccgccgc cggcgccgtc tcgtcggcgc cgttgccctt ccccacgaag
     3181 gcgtccatcg aggatgtcag gggaaggtca cgatttcgcc ggcgccttca gatcggtcgt
     3241 gagccggacc gccaggtccg tgaagaggcc ggcgagcgtc gcgcggtcgg ctttcgcatt
     3301 gcggagctcg gcgatctcct tcgaaagcgc ggtcgtcgtc tcccggttcc gcagacggat
     3361 ttcgtcggtc aggttcttgg acagatcgag cacctgctga cgaagctccc gctggctctt
     3421 cgtcgtctgc tcgtcgagct gcgcgagccg cttctcgagg ccccgcgcgg tgtcgcgaag
     3481 ctcccgggag acgtccttga cggcgccgga cctctcctcc tgctctgcct tcagccggtc
     3541 gccgagagat tcgacttcct tcttgatgaa gctctcgagc gaatcgaaac gcttcttggt
     3601 ctcggcgcgg gcgtcggacg tttccttcag cagccgctcc tcgagccgtg aaaacctctt
     3661 ctcgaagtcg tggacctgcg ctccgaaaag aatgtcccgg atcttctcga ggttggcgcc
     3721 gccgccgacc ccttcttggg cggccccttt cccggtggcc gacccgttgc ccgccttcgg
     3781 ctcctgcccg ggcgcgcctc gcttctcttc tcccggtgtg ctcatcgatc tgccctttct
     3841 gtggattttc ggtctcggtc ggttcgactt cccgggggcg tgtgctctca tggcggtcgt
     3901 cccttccccg tccgcccgcg ctcgcgcagt tttgcgtttc gagccctctg caggacggcc
     3961 tgtttctcgt cgtgaacgag agtctggttc tcgatcagga ggatcctccg agggcccacg
     4021 cagaccgcgg acgcctcgag cggcgtttcg tgcccgagcg catcgctctc gatccagatt
     4081 cccgatttca accggccgcc cgcggcgttc gtccagaaag cctcggcgtc cacgaggaag
     4141 ttgtcgagaa acgggaacga gatgctgccg gtgggagaca ggctcgcgaa ccacgcggga
     4201 ggcgcgccga ggagccggaa ccggcccccc tcctcccgct cgaaaacggc gaattcgagc
     4261 gcggcaatca ggtcggcagc gatggtcttt tcgctcattt tcgacgggcc cgctgaaaaa
     4321 gaaggcgccc acttaaccac agccgcccgg agccggcaac cgaaacgccg ggctcccgat
     4381 caggccgaga gcggcggaga tccccgacgg agcaggtaga tgacggcgcc gacggcgagc
     4441 gcgatgcctc cggtaatcca gtttttcccc tcagccgcag aaaggaacaa aagacagatg
     4501 gcgagcgcgg caagcgggat gagcggcccg ccgggaagac ggatggtccg ttcggtcgcc
     4561 ggcagcttcc gccgcagcac ggggactgcg gcggccgtgc cgatgtaggt cgcgaggcgc
     4621 gcgatcgccg agagcatcgc gaggcgctcg gcgagaccga ggaagctctt cgccggaaga
     4681 agcgcgttga tcccccaatc gacgaggatc aacatcaagg cgaccgcggt ctgggtgatg
     4741 atcgcgacgt ggggcgtccg gtagcgcgga tggaccttcg cgaacaccgc cggcagcctt
     4801 ccggtctccg cgagcgcata gaggtaccga ggtccggcga gaaccgtgtt gttgttggtg
     4861 ccgagaacgg agagcacggc gccaagggtc agaacgaggc cgccgacggg tcccatcatc
     4921 atcgccgcgg catccgcgag aggcgtcgga gacgaaccga ggttcggcac ggttccgatc
     4981 gccacgagct gcacggtcgt gtagatcgcg gtgacgatca cgatctggac gatcagcgca
     5041 aagggcacgt cgcgctttgg attgacgaac tcccccgcgg gcgcgggagt gttctcgaag
     5101 ccggcgtacg cgaagagtac gaggagcgcg gccttcgtga agttcgcacg ctcgggcgtc
     5161 ggaacgggaa acacgcggcc ccagtcgacg aagaagatcc cgaccgccac gaagagcacg
     5221 aggggcagga ccttgcccca cgcgaggacg accgcggtcc gggcgccgga cttcacgccg
     5281 aggacgttga tccacgtcag cacgaggagc ggcagaacga tcgtggcgta ctgaccgagt
     5341 cccgatcggg cgccgggcca gaggtacccg accgcgcggg cgaagaaaac cgagagcccg
     5401 gcgatcgacg cgatccgcgc gatccacgtc atccatccga cttcgaagcc gacgaagtcg
     5461 ccgaacgcgg cgcgcgtgta gacaatcgcg cctccctgcc ggtcgaagag actggaggcc
     5521 tcggcgaagc acagcacgag gagaagaacg ccgaaccccg ccgcgagaat ggcccagacg
     5581 ctcgccggac cgagaagctg ggcggccgcg acggggagga tcagatagac gccgctgccg
     5641 atgacgtcgt tgaccgacag ggcgacgatc tgccagcgcg agaccgcgcg cagcagtccc
     5701 gtcttcggat cgagacgagc gatttcgggg ctcaagcggg cgccattctc tgacgagttg
     5761 ccgagagtcc caagtccaac gtgcgaagtc ctgagtcaaa agaaaaagcc ccggtttccc
     5821 ggggcaactt tcggactctg gactttgaac ttctctatcc gttccaccgc gcgcgagtcc
     5881 cgcgggcgtc gatgtgcacg aacgggccat gggacgacgt cgccgggtag atcccgattc
     5941 ctccgatgag ctgcgggttc ttcctctcga cgacttcggc cgcggaggcg aggatgcgcg
     6001 cgtccttcag gtcgactcgt ccgtcacggt tgaggtcgtc gagccggcca tccctgtcgt
     6061 catccggata aacgtccgag gcgtcgccat actggtgacg cgaaaccgcc gac
//
LOCUS       DASWAV010000183         7866 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U2665
            k141_567746, whole genome shotgun sequence.
ACCESSION   DASWAV010000183 DASWAV010000000
VERSION     DASWAV010000183.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960316
            Sequence Read Archive: SRR10260007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 7866)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7866)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:47:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,929
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7866
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_567746"
                     /isolate="SMAG_U2665"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.36444 N 132.31889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..303
                     /locus_tag="VF958_03650"
     CDS             <1..303
                     /locus_tag="VF958_03650"
                     /inference="COORDINATES: protein motif:HMM:NF015589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 65 protein"
                     /protein_id="HEX9148234.1"
                     /translation="WTWYTGSAGWMYRAGIEWILGFRFRGAALHVDPCIPRWWPGYHI
                     TFRYHSARYELVVENPHGANCGVARIVADGRELPGEIREIPLVDDGKAHRIEVLLG"
     gene            337..2994
                     /locus_tag="VF958_03655"
     CDS             337..2994
                     /locus_tag="VF958_03655"
                     /inference="COORDINATES: protein motif:HMM:NF014625.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HEX9148235.1"
                     /translation="MLKKTFLALLLFCVCLPAARPQTAPAPGSAFEPASSGGVLALDR
                     LLQRLAVHKRMLVIGAHPDDEDNRLLTLVSRKMGAEVAYLSLSRGEGGQNLLGPHLGV
                     PLGLIRTQELLAARRIDGGRQYFTRAYDFGYTRSLDETLSLWPKDVLLEDVTRIVRRF
                     RPQVIVTVFSGTSRDGHGQHQASGVVGKEVAEADPAALHSLDKEGLLPWKISALYCCG
                     YAGPDVATIVLPTGAIDPLSGRSYHQIAMASRSNHRSQDMGALQPPGPNETRLAWVSG
                     GAGKDGRDPFAGIDTRLTAIAAGISDPTGRVRAEEHLRRVEATVGEARTDLAPAALDQ
                     TARSLATALKELREARALVPPDHDPGDAASAAFLDEKIALAETALAAAAEVTVDAVTD
                     RETVAPGDSLDVKIIVWNGGTNPARVESIELVSPDGWNIAPASPETKDLTAGTLAEWT
                     RKVSPAGAVPTIPYFLRRAMRGALYDWSDTPSIARGEPFQAPPLTALVRMRVEGTDLT
                     LSREVVNRYRDQAIGEVRRPVRAVPALEVSVDPDRIVWPIGQKDERRLEVTIASNSAK
                     PVSGNLVVVPPAGWPVISPIPFSLTGLGDRRFVDVPLRPPSPFSPGSGAFRLSAVLPG
                     GQRFSSHVEVLDYPHVPPTPMPIEATVDVVAADIRLPAVKRVGYVRGASDRVPEALTA
                     VGVPVEVLTEKDLDSGNLSRYDVIVIGSRAYETDMALVRSNNRILDYARNGGLVVVQY
                     QQYAFVEGGFAPYKLEIARPHDRVTDETAPVRVLDSKSPAFDRPNRIGSDDWNGWVQE
                     RGLYFAHTWDPAYTPLLEMTDPGQPPQRGGLLVAKLGRGLYVYTGLAFFRQLPAGVPG
                     GYRLFANLLGLQSKKHVSLKSLFSRRARR"
     gene            complement(2999..6334)
                     /locus_tag="VF958_03660"
     CDS             complement(2999..6334)
                     /locus_tag="VF958_03660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013373985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX9148236.1"
                     /translation="MKTAKLFLPFLLCLVLLAVGPPAALPIAQRPPEPVAQATPATIP
                     APTPVPTTGSGSSEAKKWNVESPPYPMPVEAPIDTDEGTWMSLDVSPDGKEIAFDLLG
                     DIYALPIGGGEARALTSGVAWDMQPRYSPDGKRIAFTSDRSGGDNVWVMDRDGSHPTP
                     VSKEDFRLLNSPVWAPDGDFLAARKHFTGTRSLGSGEIWLYHRSGGEGTQMVKRPNEQ
                     KDLGEPAFSPDGRYLYYSQDATPGRIFQYNKNPHGEIYVIQRLDRVSGRTDRFVQGPG
                     GSVRPTPSPDGKWLAFVRRVRFKTVLFTKDLVSGHERPLYDGLDRDMQETWAIHGVYP
                     SMGWTPDSRAIVVWAGGKIRRVERESGRASVIPFHVKGTRRMAEAVRFSRRIAPDRFP
                     VRMLRWVTVSPKGDQVAYQALGRIYVRDLPDGTPRRLTKQADHFELYPSFSRDGQRIV
                     YSTWNDEKAGTVRIAPSRGGEGRVVSSEPGLYREPVLSPDGARVVYRKDAGGGLLTPD
                     WSTETGLYWSPATGEAGGAAALVSRDGFGPQFGKGSDRVYFTKLEETEGSREQREEEK
                     RVFVSAALDGADLREHYVSENATEFAISPDEKWLAFRERYNAFVTPFVRTGKRIEIGP
                     KAKSLPVARVSKDAGEYIHWSGDSRSLHWALGPELFTRALPETFSFVPGAPEKLPEPE
                     EKGRPIGFDATTDVPSGSLAFVGGRVVTMKGGGDEVIEDGVVVVEGNRIRAVGPRTSI
                     PIPAGARTVDVTGKTVLPGLVDAHWHGDLGGNGVIPQQNWSTDAGLAFGVTTAHDPSH
                     DTGEFFAASEMARAGLVRAPRLFSTGTILYGAGGDVRVEIDSLDDARAHVKRMKASGA
                     FSLKSYNQPRRDQRQQILAAARELEMLVVPEGGSLFEHDMTMVVDGHTGIEHTIPVAN
                     IYKDVLQLWRSNGVGYTPTLLVGYGGIWGENYWYQHTNVWENPRLSRFVPPAELDSRS
                     MRRMMAPEEDFNHFNIARIARELHDAGVSVQVGAHGQREGLGAHWELWMLVQGGMTPL
                     EALKCGTWNGARYLGLDADIGSLEPGKLSDLVVIDGNPLADIRQSEKVLYTVVNGRLY
                     DAATMNQMGNHPGERAKYWWERD"
     gene            complement(6369..6710)
                     /locus_tag="VF958_03665"
     CDS             complement(6369..6710)
                     /locus_tag="VF958_03665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9148237.1"
                     /translation="MDALAWGIFFGVGPILWASGILLFVTSRLPLAKKVAWAAFLVAV
                     GAAIGSFLSLVSIRNRFFLLIAALPILALADVRLARSNRNFRFWFRACAFEICTVFGS
                     AAITRAVMGAI"
     gene            complement(6714..>7866)
                     /locus_tag="VF958_03670"
     CDS             complement(6714..>7866)
                     /locus_tag="VF958_03670"
                     /inference="COORDINATES: protein motif:HMM:NF024907.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="VCBS repeat-containing protein"
                     /protein_id="HEX9148238.1"
                     /translation="DDGGDRQTSKGGVRDVMRRALSGAGILLLGAGVVCGEPQAAPAR
                     FEVSTLAVGKGPGGVALADVDGDGNLDVLTANGGSRDVTLLLGDGRGRFAPAPGWPVA
                     LPIAPHVVAATAGFVAVTGHDSNDVFVIRTGRRPPVVSSFPAIAGTAPHNHGLAIADF
                     NADGTPDLATSNNNGNSVSVLLGDDRGGFRPARGSPFAVGRAPYPLAVADFNADGKAD
                     VATPDVQGDTITLLLGDGTGSLTPAPGSPYRVDHRPFFLLAARVNVDRAPDLVVSHDD
                     IDTMTILVNDGRGGFTSSRKVNVGGRTWKTGVADFDGDGRTDLVVGTAPASVAILSGD
                     GSGNFRRSGVVAVGRGPWNVAVGDLNRDGKPDVVTTSGEGDSISLLLAR"
BASE COUNT         1218 a         2766 c         2615 g         1267 t
ORIGIN      
        1 tggacgtggt acacgggatc cgcaggctgg atgtaccgcg cgggcatcga gtggatcctc
       61 gggttccgct ttcggggcgc cgcgctccac gtcgacccgt gcatcccgcg gtggtggccc
      121 ggttaccaca tcacgttccg gtatcactcc gcccgatatg agctcgtcgt cgagaacccg
      181 cacggcgcga actgcggcgt cgcccggatc gtcgcggacg gccgcgagct gccgggcgaa
      241 atccgggaga ttccgctcgt cgacgacgga aaggcccatc gcatcgaagt gctgttgggt
      301 taggtcagct tcgggactgc cttacactct ctccgcttgc tgaagaaaac ctttctcgcc
      361 ctccttcttt tctgcgtgtg cctgccggct gcccggccgc agaccgcacc ggcgcccggc
      421 agcgctttcg agcccgcttc gtccggaggc gtccttgccc tcgaccgact cctccagcgg
      481 ctcgccgtcc acaagaggat gctggtcatc ggtgcgcacc cggacgacga ggacaaccgc
      541 ctcctgactc tcgtgtcccg caagatggga gcggaggtcg cgtacctgtc gctctcgcgc
      601 ggagaggggg gacagaacct cctcgggccg catctcggcg tgccgctcgg gctcatccgg
      661 acccaggagc tcctcgcggc gcgccgcatc gacggcgggc gccagtactt cacgcgggcg
      721 tacgacttcg gctacacgag atcgctcgac gagacactct ccctctggcc gaaggacgtg
      781 ctgctcgagg acgtgacgcg gatcgtgcgg cgtttccgac cgcaggtgat cgtgaccgtc
      841 ttcagcggca cgtcgcgcga cggccacggc cagcaccagg catccggcgt cgtggggaag
      901 gaggttgccg aggcggaccc cgccgccctc catagcctcg acaaagaggg gctcctcccc
      961 tggaagattt ccgcgctgta ctgctgcggc tacgcgggcc ccgacgtcgc gacgatcgtc
     1021 ctgccgaccg gcgcgatcga tccgctctcg ggccgctcgt accaccagat cgcgatggcg
     1081 agccgcagca accaccgctc ccaggacatg ggcgctctgc agccgccggg accgaacgag
     1141 acgcggctcg cctgggtctc cggaggcgcg ggcaaggatg gcagggaccc gttcgccggg
     1201 atcgacacga ggttgaccgc gattgcggca ggcatctcgg atcctaccgg gcgcgtccgc
     1261 gcagaggagc atttgcggcg cgtcgaggcg accgtggggg aagcgcggac ggacctcgct
     1321 ccggcggcgc tcgaccagac ggcccggtca cttgcgaccg cgttgaagga gctgcgcgag
     1381 gcgcgcgcgc tcgttccacc cgaccacgac ccgggcgacg cggcgtccgc cgcgttcctg
     1441 gacgaaaaga tcgctctcgc ggaaacggct ctcgccgctg cggcagaagt gacggtcgac
     1501 gcggtcaccg accgcgagac cgtcgcaccc ggcgactcgc tcgacgtcaa aatcatcgtc
     1561 tggaacggcg ggaccaaccc cgcccgggtc gagagcatcg agctcgtgag ccccgacggg
     1621 tggaacatcg cgccggcgtc tcccgaaacg aaagatctca ccgccgggac gctcgccgag
     1681 tggactcgca aggtctctcc tgccggcgcg gtgccgacga tcccctactt ccttcgccgc
     1741 gcgatgcggg gggcgctcta cgactggagc gatacgcctt ccatcgcccg gggagagccg
     1801 ttccaggcgc cgccgctgac ggcgctcgtc cggatgcgcg tcgaaggaac cgacctcacc
     1861 ctctcccgcg aagtcgtgaa tcgctaccgc gaccaggcga tcggcgaagt tcgccggccg
     1921 gtccgcgccg ttcccgcgct cgaggtctcc gtcgatcccg accggatcgt ctggccgatc
     1981 gggcagaagg acgagcgccg cctcgaggtc acgatcgcat cgaattccgc gaagccggtc
     2041 tccgggaatc tcgtcgtcgt cccacccgcc ggttggcccg tgatttctcc gattcccttc
     2101 tccctgacgg gtctcgggga ccgccggttc gtcgacgttc ccctccggcc gccttcaccg
     2161 ttttctcccg gaagcggggc gttccggctc tccgcggtgc tccccggcgg acagcgcttc
     2221 tcgtctcacg tcgaggttct cgattacccc cacgtgccgc cgacgccgat gcccatcgag
     2281 gcgaccgtcg acgtcgtcgc cgcggacatc cgcctcccgg cggtcaagag agtcggatac
     2341 gtccgcggtg cgtcggaccg cgtgccggaa gcgctcaccg cggttggagt gccggtcgag
     2401 gtgctcaccg agaaggacct cgactccggc aatctctccc gctacgacgt catcgtgatc
     2461 ggaagccgcg cctacgaaac cgacatggcg ctcgtccgtt ccaacaaccg tatcctcgac
     2521 tacgcgcgga acggagggct cgttgtcgtc cagtaccagc agtacgcgtt cgtcgaaggc
     2581 ggcttcgcgc cctacaagct cgagatcgcg aggccgcacg accgcgtgac ggacgagacc
     2641 gcccccgtcc gcgtcctcga ctcgaagtcc cccgcgttcg atcgccccaa ccggatcggc
     2701 tccgacgact ggaacggctg ggtccaggag cggggactgt acttcgcgca cacgtgggac
     2761 ccggcgtaca cgccgctcct cgaaatgacg gacccgggcc agccgcccca gcgcggaggg
     2821 ctactcgtcg cgaagctcgg aagggggctc tacgtctaca cgggccttgc gttctttcgc
     2881 cagctcccgg ccggcgtgcc cggcggctat cgcctattcg ccaatctgct cgggctgcag
     2941 tcgaagaagc acgtttcgct caagagcctg ttctcccggc gcgcgcgccg gtagctgctc
     3001 agtctctttc ccaccagtac ttcgctctct ctcccggatg gttgcccatc tggttcatgg
     3061 tcgcggcgtc gtagaggcgc ccgttgacga ccgtgtagag aaccttctcg ctctggcgga
     3121 tgtccgccag aggatttccg tcgatgacga cgagatccga gagcttgccc ggttcgagcg
     3181 agccgatgtc ggcatcgagc ccgagatagc gcgccccgtt ccaggtcccg cacttcagcg
     3241 cctcgagcgg cgtcatccct ccctggacga gcatccagag ctcccagtgc gcgccgagac
     3301 cttcgcgctg gccgtgcgca cccacctgca ccgacacgcc ggcgtcgtgg agctccctcg
     3361 cgatccgcgc gatgttgaag tgattgaagt cctcctccgg cgccatcatt cggcgcatcg
     3421 agcgggaatc gagctcggcg ggcgggacga accgcgagag gcgcgggttc tcccagacgt
     3481 tggtgtgctg gtaccagtag ttctcccccc agatcccgcc gtatccaacg agaagcgtag
     3541 gcgtgtatcc gacgccgttc gaccgccaga gctgcaggac gtccttgtag atgttggcga
     3601 ccggaatcgt gtgctcgatc ccggtgtggc cgtcgacgac catcgtcatg tcgtgctcga
     3661 agagagaacc cccctcgggg acgacgagca tctcgagctc gcgggccgcc gcgaggatct
     3721 gctgccgctg gtcccggcgc ggctggttgt agctcttcag gctgaaggcg ccggacgcct
     3781 tcatgcgctt gacgtgcgcc cgggcgtcgt cgaggctgtc gatctcgacg cggacatctc
     3841 cccccgcccc atagaggatc gtcccggtgg agaagagtcg cggcgcgcgg acgaggccgg
     3901 cgcgtgccat ctcgctcgcc gcgaagaact cccccgtgtc gtgcgagggg tcgtgagcgg
     3961 tcgtgacgcc gaaggcgagc ccggcgtcgg tcgaccagtt ctgctgcggg atcacgccgt
     4021 tgccgccgag atcgccgtgc cagtgcgcat cgacgaggcc gggcaggacc gttttccccg
     4081 taacgtccac cgtccgggcg ccggccggga tcgggatgct cgtgcgcggc ccgaccgccc
     4141 ggatccggtt gccctcgacc acgacgacgc cgtcctcgat gacttcgtcg cctccgccct
     4201 tcatcgtgac cacgcgtccg ccgacaaagg cgagtgaccc ggagggcacg tcggtcgtcg
     4261 cgtcgaaccc gatcggcctg cctttctcct ccggttccgg gagcttctcc ggcgctccgg
     4321 gaacgaacga aaacgtctcc ggcagcgcgc gcgtaaagag ctccggcccg agcgcccagt
     4381 ggagactccg cgagtctccc gaccagtgga tgtattcgcc ggcgtccttc gagacccgcg
     4441 cgaccgggag tgacttggcc ttcgggccga tctcgatgcg cttgccggtg cgcacgaagg
     4501 gcgtcacgaa cgcgttgtaa cgctcgcgga acgcgagcca cttctcgtcg ggcgagatcg
     4561 cgaactccgt tgcgttctcg gagacgtagt gctcgcgcag atccgcgccg tcgagcgcag
     4621 cggagacgaa gacgcgcttc tcctcctcgc gctgctcgcg gctcccctcg gtctcctcga
     4681 gcttcgtgaa atagacgcgg tcgctgccct ttccgaactg gggtccgaac ccgtcccgcg
     4741 agacgagcgc ggcggctccg ccggcttccc ctgtcgcggg cgaccagtag aggccggtct
     4801 cggtggacca gtcgggcgtc aggaggccgc cgccggcgtc cttgcgatag accactcttg
     4861 cgccgtccgg cgagaggacc ggctcgcggt acaaccccgg ctcgctcgag acgacgcgtc
     4921 cttcgccgcc ccgggagggc gcaatccgca ctgtcccggc tttctcgtcg ttccatgtcg
     4981 agtacacgat cctctgcccg tcgcgcgaga aagagggata gagctcgaag tggtcggcct
     5041 gcttcgtgag gcgccgcgga gtgccgtccg gcaggtcgcg cacgtagatt cgaccgagcg
     5101 cctggtacgc gacctgatct cctttcggcg agacggtcac ccagcgcagc atccgaacgg
     5161 gaaagcggtc gggcgcgatt ctccgggaaa agcgcacggc ctccgccatc cggcgcgtcc
     5221 ccttgacgtg gaagggaatg accgacgcgc gtccgctctc gcgctcgacc cggcggatct
     5281 ttcctccggc ccagacgacg atcgcgcggc tgtccggcgt ccatcccatc gacggataga
     5341 ccccgtgaat cgcccacgtc tcctgcatgt cccggtcgag accgtcgtag aggggccgct
     5401 cgtgtccgga gacgaggtcc ttagtgaaca ggaccgtctt gaagcgcacc cggcgcacga
     5461 acgcgagcca cttgccgtcg ggcgacggcg tcggccggac cgagccgccc ggcccctgga
     5521 cgaatcggtc ggtccgaccc gagacgcggt cgagcctctg gatcacgtag atctccccgt
     5581 gcgggttctt gttgtactgg aagatccttc ccggagtggc gtcctggctg tagtagaggt
     5641 accggccgtc cggcgagaac gccggctcgc ccaggtcctt ctgctcgttg ggccgcttga
     5701 ccatctgcgt cccctcgcct cccgaacggt ggtagagcca gatctcgccc gagccgagtg
     5761 accgcgtgcc ggtgaagtgt ttgcgggcgg ccaggaaatc tccgtcgggc gcccagaccg
     5821 gactgttgag gaggcgaaag tcctctttgc tcaccggggt cggatgcgag ccgtcacggt
     5881 ccatcaccca gacgttgtcc ccacccgagc ggtccgaggt aaacgcgatc cgtttgccgt
     5941 ccggcgaata gcgcggctgc atgtcccacg cgacgcccga cgtaagcgcg cgcgcctctc
     6001 cgccgccgat cggcagcgca tagatgtcgc cgagaaggtc gaacgcgatc tccttcccgt
     6061 ccggagacac gtcgaggctc atccacgtgc cttcgtccgt gtcgatcggg gcctcgaccg
     6121 gcatcggata aggaggcgac tcgacgttcc acttcttcgc ttcggatgac ccgctcccgg
     6181 tcgtgggaac cggagtcgga gccgggatcg tggccggtgt cgcctgagcc acgggctcgg
     6241 gcggccgctg cgcgatcggg agagccgccg gcggaccgac ggcgagcagg accagacaga
     6301 gaaggaacgg caggaaaagt ttcgcggtct tcatcgctct cctcttggtc ggaattccgc
     6361 ggcagagcct atatcgcgcc catgacggcg cgcgtgatcg ccgcgctgcc gaacacggta
     6421 cagatttcga acgcgcacgc gcggaaccag aaccggaagt tgcgattcga tcgcgcgagc
     6481 cggacgtccg cgagcgcgag gatcggcagc gcggcgatca ggaggaagaa ccggtttcgg
     6541 atcgagacga gcgacaggaa cgacccgatc gccgcaccca cggctacgag aaacgcagcc
     6601 cacgcgacct tctttgccag tggcagacgc gacgtgacga aaaggaggat gccgctcgcc
     6661 cagagaatgg gcccgactcc gaagaagatt ccccaggcga gggcgtccac cgtttagcgc
     6721 gcgaggagca aggagatgct gtcgccctcg ccgctcgtcg tcacgacgtc cggtttgccg
     6781 tcgcggttca gatcgccgac ggcgacgttc caggggccgc gaccgacggc gacgacaccg
     6841 ctccgacgga agttgccgct gccgtcgccg gacagaatgg cgacggaggc gggcgcggta
     6901 ccgacgacga gatccgtccg tccgtccccg tcgaagtcgg ctacgcccgt tttccacgtc
     6961 cgtccgccga cgttcacctt tcgcgacgat gtaaatccgc cgcggccgtc gttgacgagg
     7021 atggtcatcg tgtcgatgtc gtcgtggctg acgacgaggt cgggggcgcg gtcgacgttc
     7081 accctcgctg ccaggaggaa gaaagggcgg tgatcgacgc ggtacggcga tccgggcgcc
     7141 ggcgtgagag agccggtccc atccccgaga aggagagtga tcgtgtcacc ctggacgtcc
     7201 ggcgtggcca cgtccgcctt cccgtcggca ttgaagtcgg cgaccgcgag cggatatggc
     7261 gcacgcccga ccgcgaacgg cgagccgcgg gccggacgga agccgccgcg atcgtcgccc
     7321 aacaggacgg agacactgtt cccgttgttg ttggacgtcg ccaggtcggg cgtcccgtcc
     7381 gcgttgaagt ccgcgatcgc cagaccgtgg ttgtggggag cggtcccggc gatcgcgggg
     7441 aaggacgaaa cgacgggcgg tcgccgcccg gtccggatga cgaagacgtc attgctgtcg
     7501 tggccggtga ccgcgacgaa tccggcggtc gcggcgacca cgtgcggcgc gatcggcaga
     7561 gcgaccggcc acccgggagc cggcgcgaac cggccgcgtc cgtcccccag cagaagggtg
     7621 acgtcgcggc tcccgccgtt cgcggtcagg acgtccaggt tcccgtcgcc gtcgacgtcc
     7681 gccagggcga ctcctcccgg acccttccca acggccaagg tcgagacctc gaaccgggcg
     7741 ggcgccgcct gcggctctcc gcagacgact ccggcgccga gaagaaggat cccggcgccg
     7801 gaaagcgccc tcctcatgac gtcgcgaacg ccgccttttg acgtttgtcg atctcctcca
     7861 tcgtca
//
LOCUS       DASWAV010000184         6384 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U2665
            k141_450587, whole genome shotgun sequence.
ACCESSION   DASWAV010000184 DASWAV010000000
VERSION     DASWAV010000184.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960316
            Sequence Read Archive: SRR10260007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 6384)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6384)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:47:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,929
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6384
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_450587"
                     /isolate="SMAG_U2665"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.36444 N 132.31889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..779
                     /locus_tag="VF958_03675"
     CDS             <1..779
                     /locus_tag="VF958_03675"
                     /inference="COORDINATES: protein motif:HMM:NF014874.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="cyanophycin synthetase"
                     /protein_id="HEX9148239.1"
                     /translation="GAAAVVNVDDPWGRRLAAEIPPPILTWSAQGASADVHAREIRSD
                     LSGTLLEVVHRAGRFRISSPLLGRFNVDNLLGAAAAGLALGLSEEEIAAGCSALARVP
                     GRLERVEAGQPYAILVDYAHTEDALKRLLEAVRGLTDKKIVLVFGCGGDRDRGKREPM
                     GRIAGSLSDIAIATSDNPRSEDPEAILAEVEKGLAASGATKYLKVTDRREAIRTAIGF
                     ANPGTVVVIAGKGHERVQVIGDRAIPFDDREVAAQYASKR"
     gene            831..2228
                     /gene="murF"
                     /locus_tag="VF958_03680"
     CDS             831..2228
                     /gene="murF"
                     /locus_tag="VF958_03680"
                     /EC_number="6.3.2.10"
                     /inference="COORDINATES: protein motif:HMM:TIGR01143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0047480 -
                     UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HEX9148240.1"
                     /translation="MNFTLADVAAAAGVPVPAGAADVDLNGVVVDSRAAHPGILFVAM
                     KGARVDGHDFAADAVSRGARAVLAERVPFPLPEGCPVIAVSDSSAALTALAVSLEDRA
                     GFRLAAITGSSGKTTTKEFSAAILSRRFTVERTPGNQNSAIGFPMSIANLSRVPDWMV
                     GEMGMSAKGELSRLSRAFRPHVAAITNVAPAHLASFNSIDEVAEAKAEILEGLRPEGT
                     FVANADDSRVAAIAKRWKGRVVRFGRFAKGADVTADRFERAETETRFHLRTPSGEAAI
                     VLPLPGTHQAVNFLAASAVALAAGATASDCAAAAPGLGPSPHRGEFRRHRSGALLYDD
                     SYNANPASLRAALDTLAGFAATRRVAVLGDMKELGEDEDIWHRELGKYAAERVDRLVC
                     VGELARYYGEEAIDSGFPREAIDYVASPEEATRLLDGALREGDVVLLKGSRAVGLDRT
                     VEALADSTRPVGGRR"
     gene            2240..3325
                     /gene="mraY"
                     /locus_tag="VF958_03685"
     CDS             2240..3325
                     /gene="mraY"
                     /locus_tag="VF958_03685"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015722133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008963 -
                     phospho-N-acetylmuramoyl-pentapeptide-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HEX9148241.1"
                     /translation="MLYYWLYPLAKVFPAFNVFRYITFRTAMAAVTSLLLALALGGPM
                     VRMLKRRQIGQSVRADGPRSHLAKAGTPTMGGVLILLAVIAATILWMDLKNRLVWIAL
                     GTLAGLGVVGFADDFVKITQRRSLGLSGRGKLVPQFLVAFVAAWAIEHWAASGTFSTL
                     VTFPFLKKLIIDLGAFYIPFVAVVLVGASNAVNLTDGLDGLAIGVFGVAAATYALLAY
                     VSGNAVAARYLQLTYVPQAGELSVFCGALVGASLGFLWYNSHPADVFMGDVGALPLGG
                     ALAAVAVMTKQEMLLAIVGGVFVLEALSVIIQVVSFKSTGKRVFRMAPIHHHFELAGW
                     PESRVIVRFWIMAILFSVLGLSTLKLR"
     gene            3364..4713
                     /gene="murD"
                     /locus_tag="VF958_03690"
     CDS             3364..4713
                     /gene="murD"
                     /locus_tag="VF958_03690"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008764 -
                     UDP-N-acetylmuramoylalanine-D-glutamate ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HEX9148242.1"
                     /translation="MTVLGFGLSGRALTEALVERGREVYVVEDKPSDDVEGWRDLEER
                     GVRFFFGGRSEEALDGAGWLALSPGVPPSHPVVSAAHRRGLPVLAELEIAWRIAEEEA
                     TGVNRWVAVTGTNGKSTTTAWIADILRRGARPVALAGNIGAPLSGFLQESRARDFVCE
                     VSSFQLEAVDRFRPDVAVLTNVTPDHLDRYETFEDYAAAKKRVFARQRSRDFAVVNAD
                     DPVAGRVRTSARRVPFSRGGERGGTWYEEGRLFSEVRGSRCEVLAADRLSLPGAHNLE
                     NALAAIAAAECLEAPPAAIEESLTSFRGLPHRTEIVADVAGVAWVNDSKGTNVDATAK
                     ALEGRAPKSVVLILGGRDKHGDFSKLAPQVASVARMVLTIGEAAPLIEHALAGSAPLE
                     RSGTMDHAVTRAREIARPGDTVLLSPACASFDQYRNFEERGRHFAELVRRLPRPHGS"
     gene            4703..5809
                     /gene="ftsW"
                     /locus_tag="VF958_03695"
     CDS             4703..5809
                     /gene="ftsW"
                     /locus_tag="VF958_03695"
                     /inference="COORDINATES: protein motif:HMM:TIGR02614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative lipid II flippase FtsW"
                     /protein_id="HEX9148243.1"
                     /translation="MARKLATDKVLFVALVALSLFGCVMIYSASAVSSAETSGSPYRY
                     LIKQIAALVVGGIAAFVVSRADYRLFAKPAIVYLAYGGAFALCVLALLQPPINAARRW
                     IPIGPVMFQPSEFLKVALVLLLASQIARKTLTWGDPQKAIRPALFFTMIAAGVVLLEP
                     DLGTAACYVMLAGVLLWLAGVRAKLFLMLGAAAVPLVVAAALTADYRRARLLSFLRPE
                     ADPLGAGFQALQSLIAVGAGGFLGNGLGASRQKLFFLPYPHTDFIFAIVGEELGFLGA
                     VGVVLCFAVVAWRGFRAARRAPDAFGALVAAGATAMLVVQAAVNMSVVLALVPTKGIP
                     LPFVSYGGSSLVASWIAAGLILNVSQHEVEIRET"
     gene            5806..>6384
                     /locus_tag="VF958_03700"
     CDS             5806..>6384
                     /locus_tag="VF958_03700"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015022.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016758 - hexosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0030259 - lipid glycosylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX9148244.1"
                     /translation="MTREPDGGAYVIAAGGTGGHIFPAIALAREIRSRRPEAEIVFIG
                     SRHGLETKLVPQAGFPLELVRASGFAGKSAGAMLGALAEVPVGFMEARRILAERRARA
                     VAGVGGYVSVPVVLAARSLRVPTLIHESNAFPGVANRLLNRFATRTAVGVAAANSRLA
                     RPGVVTGTPVRPEFFRVPPMDPGSATRRLLVFG"
BASE COUNT          854 a         2212 c         2216 g         1102 t
ORIGIN      
        1 cgggcgccgc ggcggtcgtg aacgtcgacg atccctgggg gcgccggctc gccgcggaga
       61 tccctccacc gatcctcacg tggtccgcgc agggcgccag cgcggacgtc cacgcccgcg
      121 agatccgttc cgatctttcc ggcacgttgc tcgaggtcgt tcaccgcgcg ggcagatttc
      181 gaatctcctc gccgctcctc gggcgcttca acgttgataa tcttctcgga gccgcagcgg
      241 cgggactcgc gctcgggttg agcgaggaag agatcgccgc gggttgctcg gcgctcgcgc
      301 gcgtgcccgg ccgactcgag cgggtggaag cggggcagcc ctacgcgatc ctggtggact
      361 acgctcacac cgaagacgcg ttgaagcggc tgctcgaggc ggtgcgggga ctgacggaca
      421 agaagatcgt tctcgtgttc gggtgtggcg gcgaccgcga ccgcggaaag cgagagccga
      481 tgggccggat cgcggggtcg ctttcggaca tcgcgatcgc gacgtccgac aatcctcggt
      541 cggaggatcc ggaggcgatc ctcgccgagg tcgagaaggg actcgccgcg tccggcgcga
      601 cgaagtacct gaaagtgacc gaccgccgcg aggcgatccg taccgcgatc ggtttcgcga
      661 accccggcac ggtcgtcgtg atcgccggca agggccacga gcgcgtccag gtcatcggag
      721 accgggcgat cccgttcgac gatcgagagg tggccgccca atatgcgtcg aagcggtgag
      781 tttcgtaatc gggacgtcgc ggtgagccct cgcgtgatgg aagcgttctg atgaacttca
      841 ctctcgccga cgttgccgcc gccgcgggcg tcccggtgcc tgccggagcc gcagacgtcg
      901 acctgaacgg agtcgtcgtc gactcccgcg cggcgcaccc cggaatcctt ttcgtcgcga
      961 tgaagggagc gcgggtcgac ggccacgact tcgcggccga cgccgtgtcg cgcggcgccc
     1021 gcgcggtgct ggccgagcgc gtcccgttcc ccctgccgga aggctgtccg gtcatcgcgg
     1081 tgtcggactc ctccgcggcg ctcacggcgc tcgccgtctc gctcgaagat cgcgcgggat
     1141 tccggctcgc cgcgattacc ggttcctcgg ggaagacgac gacgaaggag ttttccgcgg
     1201 cgatcctctc tcggcggttc accgtcgaac ggactccggg caaccagaac agcgcgatcg
     1261 gctttccgat gtcgatcgca aacctctctc gcgtgcccga ctggatggtg ggcgaaatgg
     1321 ggatgagcgc gaagggggag ctctcgcggc tctcgcgcgc cttccgtcct cacgtcgcag
     1381 ccatcacaaa cgtcgccccc gcgcacctgg cgtccttcaa ctcgatcgac gaggtcgcgg
     1441 aggcgaaggc ggagatcctg gagggcctcc ggccggaggg cacgttcgtc gcgaacgcgg
     1501 acgactcgcg ggtggcggcg atcgcgaagc gctggaaagg ccgggtcgtc cggttcgggc
     1561 gcttcgccaa gggtgccgac gtcacggccg atcgcttcga gcgcgccgag acggagaccc
     1621 gtttccatct ccgtactccg tcgggcgagg ccgcgatcgt cctgccactt ccgggcacgc
     1681 accaggcggt caatttcctc gccgcctccg cggtcgcgct cgccgcgggc gccaccgcgt
     1741 ccgactgcgc tgccgcggcg cccgggctcg gtccctcgcc ccaccgcgga gagtttcgcc
     1801 gacaccgctc gggcgctctc ctctacgacg actcctacaa cgcgaacccc gcttcgctca
     1861 gggccgcgct cgacacgctg gcgggcttcg cggcgactcg gagggtcgcg gttctcggcg
     1921 acatgaagga gctgggagaa gacgaagaca tctggcaccg cgagctcgga aagtacgccg
     1981 ccgagcgcgt cgaccggctc gtgtgcgtcg gcgagcttgc ccgttactac ggcgaagagg
     2041 cgatcgacag cggttttccg cgggaggcga tcgattacgt cgcttcgccc gaggaggcga
     2101 cgcgcctgtt ggacggcgcc ctgcgcgaag gggacgtcgt cctcctcaag ggctcgcgcg
     2161 cggtggggct cgatcggacc gtggaagcgc tcgccgactc gacccgcccg gtcggcggga
     2221 gacgctaggc gacaggccga tgctctatta ctggctctac ccgctcgcca aggttttccc
     2281 ggcgttcaac gtcttccgat acatcacgtt tcggacggcg atggccgccg tcacgtcgct
     2341 ccttctcgcg ctcgcgctcg gcggcccgat ggtccgcatg ctgaagcggc ggcagatcgg
     2401 gcaatcggtc cgcgcggacg gaccgcgctc ccatctcgcg aaagccggga ctccgacgat
     2461 gggcggcgtc ctcatcctcc tggcggtcat cgcggcgacg atcctgtgga tggacctgaa
     2521 gaaccggctc gtctggatcg cgctcgggac gctcgcgggc ctcggagtcg tcggctttgc
     2581 ggacgacttc gtcaagatca cgcagcggcg cagcctcggg ttgtcgggcc gtggaaagct
     2641 cgtgccgcag tttctcgtcg cattcgtcgc cgcgtgggcg atcgagcact gggccgcgtc
     2701 cggcacgttc tccaccctcg tcacctttcc atttctcaag aagctcatca tcgatctcgg
     2761 cgccttctac atcccgttcg tcgcggtggt gctcgtcggg gcttcgaacg ccgtcaatct
     2821 caccgacggc ctggacggcc tcgcgatcgg cgtcttcggc gtcgcggctg cgacctacgc
     2881 gctcctggcg tacgtcagcg gcaacgcggt ggcggcccgc tacctccagc tcacgtacgt
     2941 gccgcaggcc ggagagctct ccgttttctg tggagcgctc gtgggcgcct cactcgggtt
     3001 cctctggtac aactcgcatc ccgccgacgt tttcatgggt gacgtcggcg cccttcctct
     3061 cggtggcgcg ctcgccgccg tcgcggtgat gacgaagcag gagatgctgc tcgcgatcgt
     3121 cggcggggtg ttcgtgctcg aggcgctctc ggtgatcatc caggtcgtct ctttcaagtc
     3181 gaccggcaag agggtgtttc gaatggcgcc catccaccat cacttcgagc tcgcgggctg
     3241 gccggaatcg cgcgtgatcg tccggttctg gatcatggca atcctcttct ccgtgctcgg
     3301 tctttccacg ctgaaactcc gatgacgtcg cccgcagttc gttcggctga ccttccggcg
     3361 cgggtgaccg ttctcggttt cggtttgagc ggccgcgcgt tgacggaggc actcgtcgag
     3421 cgcgggcggg aggtctacgt cgtcgaggac aagccgtcgg acgatgtcga aggctggcgc
     3481 gacctcgagg agcgcggcgt gcggttcttc ttcggcgggc ggtcggagga agcgctcgac
     3541 ggcgcgggct ggctcgcgct gtcccccggg gtgccgccgt cgcatccggt cgtctcggcg
     3601 gcgcaccgac gcgggctgcc ggtcctcgcg gagctcgaga tcgcctggcg aatcgccgag
     3661 gaggaagcga cgggagtcaa tcggtgggtc gcagtcacgg ggacgaacgg aaagtcgacg
     3721 acgacggcgt ggatcgcgga catcctgcgc cgcggagcgc ggccggtcgc tctcgccggg
     3781 aacatcggcg cgccgctttc cggctttctc caggagagcc gggcgcgcga cttcgtgtgc
     3841 gaggtctctt cattccagct cgaggcggtc gaccgctttc ggcccgacgt cgcggtgctc
     3901 acgaacgtca cccccgacca cctcgatcgt tacgagacgt tcgaggacta cgccgccgcc
     3961 aagaagcggg tcttcgcgag acagcgctcc agggacttcg cggtcgtcaa tgccgacgac
     4021 ccggtcgcgg gccgcgtgcg gacgtcggcg cgccgcgtcc ccttctcgcg cgggggcgag
     4081 cgcggcggaa cctggtacga agaaggccgg ctcttctcgg aggtccgcgg ctcgaggtgc
     4141 gaggtgctcg cagccgatcg gctttccctg cccggcgcgc acaatctgga gaacgctctc
     4201 gcggcgatcg cggccgccga gtgcctcgaa gctcccccgg ccgcgatcga ggagagcctg
     4261 acgtccttcc gcggcctgcc tcaccgcacc gagatcgtcg cggacgtcgc cggtgtcgcc
     4321 tgggtgaacg actcgaaggg aaccaacgtg gatgcgaccg ccaaggcgct cgagggccgc
     4381 gcgccgaaga gcgtcgtcct cattctcggc gggcgcgaca agcacggcga cttctcgaag
     4441 ctcgctccgc aggtcgcgag cgtcgcccgc atggtgctta cgatcgggga agccgctccg
     4501 ttgatcgagc acgcgctcgc cggaagcgca cccctggagc gcagtggaac gatggaccac
     4561 gcggtgacgc gcgcgagaga gatcgcgcga cccggcgaca ccgttcttct gtcgcccgcg
     4621 tgcgcgtcgt tcgaccagta ccggaacttc gaagagcgcg gaagacactt cgccgagctc
     4681 gtgcggcgtc tgccgaggcc ccatggctcg taagctcgcc accgacaagg tccttttcgt
     4741 ggcgctcgtc gcgctgtcgc tcttcggctg cgtgatgatc tacagcgcct cggcggtctc
     4801 ttcggcggag acctccggca gcccttaccg ctacctcatc aagcagatcg cggccctcgt
     4861 cgtcgggggg atcgcggcgt tcgtcgtctc gcgtgctgac taccggctct tcgccaaacc
     4921 cgcgatcgtt tacctcgcct acggcggtgc gttcgcgctc tgcgtgctcg cgctccttca
     4981 gccgccgatc aacgccgcgc gccgctggat tcccatcggg cccgtgatgt tccagccctc
     5041 ggagttcctg aaggtcgcgc tcgtgctcct gctggcgtct cagatcgccc gcaagaccct
     5101 gacctgggga gacccgcaga aggcaatccg ccccgccctc ttcttcacca tgatcgccgc
     5161 gggagtcgtc ttgctcgagc cggacctcgg gactgcggcg tgttacgtca tgctggccgg
     5221 cgtcctcctc tggctggcgg gcgtgcgcgc gaagctgttc ctcatgctcg gagcggcggc
     5281 ggtcccgctc gtggtcgcgg cggccctgac cgccgactac cggcgggcgc gtctgctgtc
     5341 gttcctccgg ccggaggcgg accccctggg cgcggggttc caggcgctgc agtcgctgat
     5401 cgccgtcggt gcgggagggt tcctgggaaa cggcctcgga gcgagtcgcc agaaactctt
     5461 cttcctgccc tatccccaca ccgatttcat tttcgcgatc gtcggcgaag agctcggctt
     5521 cctgggagcc gtcggggtcg tgctctgctt cgccgtcgtc gcgtggcgtg gcttccgcgc
     5581 cgctcggcgc gcgccggacg cgttcggcgc gctcgtcgcg gcgggagcga cggcgatgct
     5641 cgtcgtgcag gcggcggtga acatgagcgt cgtcctcgcg ctcgtgccga cgaagggaat
     5701 tccgctgccc ttcgtctcct acggaggatc gtctctcgtc gcttcctgga tcgccgcagg
     5761 gctgatcttg aacgtgtcgc aacacgaagt ggaaatccgc gagacgtgac gcgtgagccg
     5821 gacggaggtg cgtacgtgat cgcggcgggt ggtacgggcg gacacatctt ccctgcgatc
     5881 gcgctcgcac gcgagatccg cagccggcgc cccgaggcgg aaatcgtctt catcggaagc
     5941 cgccacggcc tcgagacgaa gctcgtcccg caggccggct tcccgctcga gctcgtccgc
     6001 gcttccggtt tcgcggggaa gtccgccggg gccatgctcg gcgcgctcgc ggaggtgccc
     6061 gtcgggttca tggaagcgcg ccggatcctc gcggagcgcc gggcgcgcgc ggtggccggc
     6121 gtcggcgggt acgtgagcgt cccggtcgtg ctggccgcgc ggtcgctccg agtgccgact
     6181 ctgatccacg agtccaacgc cttccccggc gtggccaacc ggctgctgaa ccggttcgcc
     6241 acccgaacgg ccgtcggcgt ggcggcggcc aattcgcgcc tcgcgcggcc cggggtcgtc
     6301 accggcactc ccgtgcggcc cgagttcttc cgcgtcccgc cgatggaccc ggggtccgcg
     6361 acccggcggc tgctcgtctt cgga
//
LOCUS       DASWAV010000185         3278 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U2665
            k141_491134, whole genome shotgun sequence.
ACCESSION   DASWAV010000185 DASWAV010000000
VERSION     DASWAV010000185.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960316
            Sequence Read Archive: SRR10260007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 3278)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3278)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:47:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,929
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3278
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_491134"
                     /isolate="SMAG_U2665"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.36444 N 132.31889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..239)
                     /locus_tag="VF958_03705"
     CDS             complement(<1..239)
                     /locus_tag="VF958_03705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9148245.1"
                     /translation="MSMRKVVLVALALGSMAGLAVAASEKGGSEAQVRARSQEFAASW
                     NRHDPKAMAGVWAADGDLINPFGRTAKGRNEIEKLL"
     gene            376..798
                     /locus_tag="VF958_03710"
     CDS             376..798
                     /locus_tag="VF958_03710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008464474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase"
                     /protein_id="HEX9148246.1"
                     /translation="MKVRLVSASFFSAAHRLHRDDWDAETNRRVFEKCNNPNAHGHTY
                     GLEVTVEGEIDPETGWVMDFGELKRTVHERVIRKVDRKHLNFDVDFLAGRNPTAENIV
                     IGIWGELVDRVAPARLVRVTLNETEKNRVVYEGGSNER"
     gene            788..1006
                     /locus_tag="VF958_03715"
     CDS             788..1006
                     /locus_tag="VF958_03715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013842671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0080135 - regulation of cellular response
                     to stress [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HEX9148247.1"
                     /translation="MNAEPKPRRLTRSNRSKMIAGVCGGLAEYLNMDPTVMRVLYVLV
                     SVMSAAFPGVIAYIVLMFLMPPPEEVTA"
     gene            1003..1215
                     /locus_tag="VF958_03720"
     CDS             1003..1215
                     /locus_tag="VF958_03720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003473009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0080135 - regulation of cellular response
                     to stress [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HEX9148248.1"
                     /translation="MSSPSPHPVPLRRSRTDRMIAGVCAGLAHHFALDVSLVRVLYVL
                     VSILSAAFPGILVYIILWIVIPEESA"
     gene            1212..2006
                     /gene="surE"
                     /locus_tag="VF958_03725"
     CDS             1212..2006
                     /gene="surE"
                     /locus_tag="VF958_03725"
                     /EC_number="3.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004511648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008252 - nucleotidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HEX9148249.1"
                     /translation="MTGPRILVTNDDGVFSEGIERLSAALLEVGDVYTVAPDQERSAA
                     GHALTLDTPLRAKEISEKRWSVNGTPTDCVNWGVLHLLRDSRPRLLFSGINLGLNLGD
                     DVTYSGTVSAAFEGALMGIPSVAISQEIERGFTFDAAAEFAARLARRLLEQPLPPKTL
                     INANVPAGSVRGIRVRRLGTRRYGETVIEKTDPRGRPYYWIGSTIPVGELEEGTDISA
                     VAEKFISMTPVHLDLTAYQSMELLETIAAEVSPRETLTPGRIRTST"
     gene            complement(2053..3213)
                     /locus_tag="VF958_03730"
     CDS             complement(2053..3213)
                     /locus_tag="VF958_03730"
                     /inference="COORDINATES: protein motif:HMM:NF018884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HEX9148250.1"
                     /translation="MAGLGPDDGEAASPFGAGPSAKLVAFLGRQPRWRITALGVVLLG
                     GLWVIDRATAPDLSFLVFYLAPVLLLTWFVGHRAGMLMSLASAVLWFADDVLTAPAPA
                     RPLTPYWNAAVKLGFFLFVVQILSSLKAALERQRQAERERIDLELAMAAQVQKGLFPQ
                     KLVPVTGLDYFGICRPAAAVGGDYYDFIPLGPERLGIAVGDISGKGLPASLLMASLQG
                     SLRSWASSTPGDEARTIEQVNRQLCALTESARFATLFWGVYDAGRRELAHVNAGHNPP
                     MLFRRADGFILTTKRLTIGGTVLGMFPEAEFRRDVVALEPDDLLVLFSDGIPEAPSSE
                     VEFGETRLETFLRSNASVPPADLCERVLAEVRSYLGEQPTPDDLTIVVLKVR"
BASE COUNT          576 a         1119 c         1042 g          541 t
ORIGIN      
        1 aggagcttct cgatctcgtt gcgtcccttc gccgtccggc cgaacgggtt gatgaggtca
       61 ccgtccgccg cccagacgcc agccatcgct ttggggtcgt gccgattcca gcttgccgcg
      121 aattcctggc tccgggcgcg cacctgagcc tcgcttccgc ccttctccga agccgccacc
      181 gcaagacccg ccatggagcc cagcgccagc gcgaccagca ctactttccg catactcatg
      241 cccctttcac cgccaacagc atctagattt tctcgacttt ccgcgcggcg attagatcac
      301 gaggtccgaa gcgttctgag ccgccgccgc cgctccagcc gcccgggtcc ccccatccgg
      361 cgcataattc gcgcggtgaa agttcggctc gtctccgcct cctttttctc ggcggctcac
      421 cgcctgcacc gggacgactg ggacgccgaa acgaaccgcc gcgtattcga gaagtgcaac
      481 aacccgaacg cgcacgggca cacgtacggg ctcgaggtaa cggtcgaggg ggagatcgac
      541 cccgagaccg gttgggtgat ggacttcggg gagctcaagc gcaccgtcca cgagcgcgtt
      601 atccgcaagg tcgatcggaa gcacctgaat ttcgacgtgg atttcctcgc gggacggaac
      661 ccgaccgccg aaaacatcgt tatcggtatc tggggggagc tcgtcgaccg cgtcgccccg
      721 gcgcgcctcg tgcgggtcac cctgaacgag accgagaaga accgcgtcgt ctatgaagga
      781 gggagcaatg aacgctgaac cgaagccccg ccggctgacg cgatcgaacc gcagcaagat
      841 gatcgccggg gtctgcggtg gactggccga gtacttgaac atggacccca cggtcatgcg
      901 cgtgctctac gtgctcgtgt cggtcatgtc cgcggccttt ccgggggtca tcgcgtacat
      961 tgtcctgatg ttcctgatgc cgccccccga ggaggtgacc gcatgagctc tccgtctccg
     1021 cacccggtcc ccctgcgccg gtcccggacc gaccggatga tcgccggcgt ctgcgccggc
     1081 ctcgcccatc acttcgccct cgacgtctcg ctcgtgcgcg tcctgtatgt gctcgtctcg
     1141 atcctctcgg ccgcgtttcc cgggatcctc gtctacatca tcctgtggat cgtgattccc
     1201 gaagaaagcg cgtgaccggt ccccgcatcc tcgtcacgaa cgacgacggg gtcttttcgg
     1261 aagggatcga gcggctctcg gcggcgcttc tggaggttgg cgacgtctat acggtcgcac
     1321 ccgaccagga gcgcagcgcc gcgggtcacg ccctgacgct cgacacgccc ctccgagcca
     1381 aggagatctc cgagaaacgc tggtcggtca acggcacccc gacggactgc gtgaactggg
     1441 gcgtgctcca tctgctccgc gattcgcgcc cccggcttct cttttccggc atcaacctgg
     1501 ggctgaacct tggagacgac gtgacgtact cgggcaccgt ttcggcggcg ttcgaaggcg
     1561 cgctcatggg cattccatcg gtcgcgattt cgcaggagat cgaacgggga ttcacgttcg
     1621 acgccgccgc ggagttcgcg gcccggctgg cgaggcgcct cctcgagcag ccactgcctc
     1681 cgaagaccct catcaacgcc aacgttccgg ccggctccgt ccgcggcatc cgcgtccgac
     1741 ggctcggcac gcggcgctac ggcgagacgg tcatcgaaaa gaccgacccg cggggaaggc
     1801 cgtactactg gatcggctcg acgatcccgg tcggcgagct cgaggaggga accgacatca
     1861 gcgccgtcgc ggagaagttc atctcgatga ccccggtcca cctcgacctg accgcgtacc
     1921 agtcgatgga gctgctcgag acgatcgccg cggaggtgtc gccgcgcgag accctgaccc
     1981 cgggacgaat ccgaaccagt acctgagcgt ccgccctatt catcgaatct cttcctgccg
     2041 cgcggggctg gctcaccgca ctttcagcac gacgattgtc aggtcatcgg gggtcggctg
     2101 ttctccgaga tagctccgga cctccgcgag aactcgctcg caaaggtcgg ccggcggcac
     2161 tgacgcattt gagcggagga aggtctccag gcgcgtctcg ccgaattcca cctcggagct
     2221 gggggcctcg gggataccgt cgctgaagag caccaggaga tcatcgggct ccaacgcgac
     2281 gacatcgcgc cggaactccg cttccgggaa catcccgagg accgtgccgc cgatcgtgag
     2341 ccgcttcgtg gtgaggatga atccgtccgc gcgacggaac agcatcggcg gattgtgccc
     2401 ggcgttgacg tgggcgagct cgcgacgccc ggcgtcatag accccccaga agagcgtcgc
     2461 gaaccgcgca ctttcggtga gggcacagag ttgccgattc acctgctcga tggtgcgtgc
     2521 ttcgtcgccc ggggtagatg acgcccagct cctcagagat ccctgcaggc tcgccatcag
     2581 gagcgaggcc ggcaggccct tgccggagat gtcgccgacc gcgatcccga gcctttccgg
     2641 ccccagtgga atgaagtcgt agtaatcgcc cccgacggcg gccgcgggac gacagatccc
     2701 gaaatagtcg aggccggtga ccgggacgag cttctgtgga aagagcccct tctggacctg
     2761 ggcggccatc gcgagctcga gatcgattcg ctcccgttcc gcctgcctct gtctctccag
     2821 cgccgccttc agggaggaga ggatctggac gacgaagagg aagaacccga gcttgaccgc
     2881 agcgttccag tagggtgtga gcggccgggc gggggccgga gcggtgagga cgtcgtctgc
     2941 aaaccagagg accgcgctcg cgagcgacat gagcatgccg gcgcggtgtc cgacgaacca
     3001 ggtgaggaga agcaccggcg caaggtagaa gacgaggaaa ctcaagtccg gcgcggtcgc
     3061 tcgatcgatg acccacaggc cgccgagaag gactacgcca agggccgtga tccgccagcg
     3121 cggctgtcgc ccgaggaacg cgacgagttt cgccgaagga cccgccccga agggagaagc
     3181 ggcctctcca tcgtccggtc cgagccctgc cacagacatt cgaaccggga aacgtgatca
     3241 gtaggccaaa accttccgcg cgacagcgag gaccttct
//
LOCUS       DASWAV010000186         3625 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U2665
            k141_650093, whole genome shotgun sequence.
ACCESSION   DASWAV010000186 DASWAV010000000
VERSION     DASWAV010000186.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960316
            Sequence Read Archive: SRR10260007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 3625)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3625)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:47:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,929
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3625
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_650093"
                     /isolate="SMAG_U2665"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.36444 N 132.31889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..684)
                     /locus_tag="VF958_03735"
     CDS             complement(<1..684)
                     /locus_tag="VF958_03735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013566957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HEX9148251.1"
                     /translation="MDTIAFAAKPTTLKDFNAIKISLASPEKIRSWSYGEVTKPETIN
                     YRTFKPERDGLFCAKIFGPITDWECLCGKYKRMKHRGVVCDKCGVEVTKSKVRRERMG
                     HIELASPVSHVWFFKGLPSRIGHLLDISLRDLERILYFESYVVVDPGDTDLKEKELLS
                     EERYRALRAEHGDVFVARMGAGAIKELLQRVDVDMLAEELRMIMKTETSQIKRQKAAK
                     RLKVCDAFRR"
     gene            complement(713..>3625)
                     /gene="rpoB"
                     /locus_tag="VF958_03740"
     CDS             complement(713..>3625)
                     /gene="rpoB"
                     /locus_tag="VF958_03740"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011687143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HEX9148252.1"
                     /translation="NLFEGMFFDPKKYDFSRVGRFKFNIKLEADSSEDTRTLTSQDIY
                     RVVAYLLRLKRDVGRTDDIDNLGNRRVRSVGELLENQFRIGLVRMERAIKEKMSVHQD
                     IDSAMPHDLINSKPVIASIKEFYGSSQLSQFMDQTNPLSEVTHKRRLSALGPGGLSRE
                     RAGFEVRDVHPTHYGRVCPIETPEGPNIGLISSLSCYARINDFGFIETPYKRVEKGRV
                     LDHFQVTQAGDTPFKLAQIVEKRELDEENEKARRARKNAADAEPYAFYLSAWEEENFV
                     IAQANAETDASGRIREDRVIARFNGEFITAEREKVDYKDISPKQLVSVATALIPFLEH
                     DDANRALMGSNMQRQAVPLLEVEAPLVGTGLEGTVARDSGAVILAKRGGVVDQVDSER
                     IIVRVEGGEETGGGKEFGADIYPLTKFRRSNQNTCINQKPIVKEGTRVARGEVLADGP
                     CTSQGELALGRNVLVAFMPWRGYNYEDAILVSEKLVKDDFFTSIHIQEFDIEARDTKL
                     GPEEITRDIPNVSENALRDLDESGIIRIGATVKPGDILVGKVTPKGETQLTPEEKLLR
                     AIFGEKAGDVRDASLKCPPGIEGTIVDVKIFSRKGIDKDLRALAIEQDQIAKIEKNSK
                     DEIRIIREEANKKIRDLLVGKVAADEVNDREGSELVKKGGRFTVEAVARLSPQQLKRL
                     KLKDEEVADAVQMILRRQDSQIEVLNRVNAERIDLLQKGDELPPGVIKMVKVYVAMKR
                     KLSVGDKMAGRHGNKGVISRILAEADMPYLPDGTPIEIVLNPLGVPSRMNVGQILETH
                     LGWAVRALGLSVATPVFDGASESEIKKLLREANLPTSGKTVLYDGMTGEPFEQQVTVG
                     YIYMMKLSHLVDDKIHARSIGPYSLITQQPLGGKAQFGGQRFGEMEVWALEAYGASYT
                     LQELLTVKSDDVEGRSQVYEAIVKGEVPADPGLPESFNVLVRELQSLCLDVELLKK"
BASE COUNT          564 a         1167 c         1151 g          743 t
ORIGIN      
        1 gcggcggaac gcgtcgcaga ccttcaggcg cttggccgcc ttctggcgct tgatctgcga
       61 agtttccgtt ttcatgatca tgcggagctc ttcggcgagc atgtcgacgt cgacccgctg
      121 gaggagctcc ttgatggccc cggctcccat gcgggcgacg aagacgtcgc cgtgctccgc
      181 ccgcagcgcc cggtaacgct cttcggagag cagttccttt tccttcaggt cggtgtcgcc
      241 cgggtcgacg acgacgtacg actcgaagta gaggattctc tcgaggtcac gaagcgagat
      301 gtcgagcagg tggccgatcc gcgacggcag gcccttgaag aaccagacgt gggaaacggg
      361 cgacgccagc tcgatgtggc ccatgcgctc gcggcggacc ttcgacttgg tcacctcgac
      421 gccgcacttg tcgcagacga cgccgcggtg cttcatgcgc ttgtacttgc cgcagaggca
      481 ctcccagtcg gtgatcgggc cgaagatctt cgcgcagaac agcccgtcgc gctccggctt
      541 gaaggtccgg tagttgatcg tctcgggctt ggtgacctcg ccgtaagacc acgaccggat
      601 cttctcgggc gacgccagag agatcttgat cgcgttgaag tccttgagcg tcgtcggttt
      661 cgctgcgaaa gcgattgtgt ccatgaggct ccttatgcgc ttcccgcgtt catcacttct
      721 tcaacagttc cacgtcgagg cagagcgact ggagctcccg cacgaggacg ttgaaggact
      781 cgggcaggcc cggatccgcc gggacttcgc ccttcacgat cgcctcgtag acctgcgacc
      841 gcccttcgac gtcgtcggac ttcacggtca ggagctcctg cagcgtgtag gaagctccgt
      901 atgcttcgag cgcccagacc tccatctcgc cgaatcgctg gccgccgaac tgcgctttcc
      961 cgccgagcgg ttgctgcgtg atgagcgagt acggcccgat cgaccgcgcg tggatcttgt
     1021 cgtcgaccag atgcgagagc ttcatcatgt agatgtaacc gacggtgacc tgctgttcga
     1081 aaggctcacc cgtcatgccg tcgtagagaa cggtcttgcc cgaggtcggc aggttcgctt
     1141 cgcgcaggag cttcttgatc tccgactcgg aggcgccgtc gaaaacgggc gtcgccacgg
     1201 acaggccgag cgcgcgcacc gcccacccga ggtgggtctc caggatctgc ccgacgttca
     1261 tgcgggaggg aactccgagc gggttcagca cgatctcgat cggcgtgccg tcgggcaggt
     1321 agggcatgtc ggcttcggcg aggatgcgcg agatgacgcc cttgttgccg tgccgtccgg
     1381 ccatcttgtc gccgacggag agcttgcgct tcatcgcgac gtagaccttg accatcttga
     1441 tgacgcctgg gggcagctcg tcgcccttct ggagcagatc gatccgctcc gcgttcaccc
     1501 ggttcagaac ctcgatctgg gagtcctggc gccgcaggat catctgcacg gcgtcagcga
     1561 cctcctcgtc cttcagtttc agacgcttca gctgctgggg cgagagccgc gcgaccgcct
     1621 cgacggtgaa gcgtccgccc ttcttgacga gttcgctgcc ctcgcggtcg ttaacctcgt
     1681 ccgcggcgac ctttccgacg agcaggtcgc ggatcttctt gttcgcctcc tcgcggatga
     1741 tccggatctc gtccttcgag ttcttctcga tcttcgcgat ctgatcctgc tcgatggcga
     1801 gcgcgcggag gtccttgtcg atgcccttcc tcgagaagat cttcacgtcg acgatggtgc
     1861 cctcgatgcc cggcggacac ttcagcgacg cgtcgcgcac gtccccggcc ttctcgccga
     1921 aaatcgcccg gaggagcttc tcttcggggg tgagctgggt ctcccctttc ggcgtgacct
     1981 ttccgacgag gatgtctccg ggcttgaccg tcgcgccgat gcggatgatc cccgactcgt
     2041 cgagatcccg aagcgcgttc tccgacacat tggggatgtc gcgcgtgatc tcttcgggtc
     2101 ccaacttggt gtcccgcgcc tcgatgtcga actcctggat gtggatcgac gtgaagaagt
     2161 cgtccttgac gagcttctcc gaaacgagga tcgcgtcctc gtagttgtat ccccgccacg
     2221 gcatgaacgc gacgagcacg ttccttccga gcgcgagttc gccctgggag gtgcacggcc
     2281 cgtcggcaag gacttcgcca cgagcgaccc gcgtcccctc cttgacgatc ggcttctggt
     2341 tgatgcacgt gttctggttc gaccgccgga acttcgtcag cgggtagatg tcggctccga
     2401 actccttgcc gccgccagtc tcctcgcccc cctcgacgcg gacgatgatt cgctccgagt
     2461 cgacctgatc gaccaccccg ccgcgcttgg cgagaatgac cgcgccggag tcgcgagcga
     2521 cggtgccttc gaggccggtc ccgacgagcg gggcctccac ctcgaggagc ggcaccgcct
     2581 gtcgctgcat gttcgaaccc atgagcgccc ggttcgcgtc gtcgtgttcc aggaacggaa
     2641 tgagggcggt cgcgaccgag acgagctgct tgggtgagat gtccttgtag tcgaccttct
     2701 cgcgctccgc cgtgatgaac tcgccgttga agcgggcgat gacccgatcc tcccggatcc
     2761 gaccggatgc gtccgtctcc gcgtttgcct gcgcgatgac gaagttctct tcttcccacg
     2821 cggagagata gaaggcgtac ggctccgcgt cggccgcgtt cttcctggcg cggcgcgctt
     2881 tctcgttctc ttcgtcgagc tcgcgctttt caacgatctg ggcgagcttg aatggcgtgt
     2941 cgcccgcctg cgtgacctgg aagtgatcga ggacgcggcc tttctctacc ctcttgtagg
     3001 gcgtctcgat gaaaccgaag tcgttgatgc gcgcgtagca cgagagcgac gagatcaggc
     3061 cgatgttggg accttccggc gtctcgatcg ggcagacgcg gccgtaatgc gtcggatgga
     3121 cgtcgcgcac ctcgaaacct gcgcgctccc gcgagaggcc gcccggcccg agggccgaga
     3181 ggcgccgctt gtgcgtgacc tcggagagcg ggttcgtctg atccatgaac tgcgagagct
     3241 gggacgaacc gtagaactcc ttgatcgagg cgatcaccgg cttcgagttg atcaagtcgt
     3301 gcggcatggc cgagtcgatg tcctggtgca cggacatctt ttccttgatg gcgcgctcca
     3361 tccgcacgag gccgatccgg aactggttct cgagcagctc gccgaccgag cggacccggc
     3421 ggttgccgag gttgtcgatg tcgtcggtgc ggccgacgtc cctcttcagc ctcagcaggt
     3481 acgcaacgac gcggtagatg tcctgtgacg tgagcgtccg cgtgtcctcg gaggagtcgg
     3541 cttcgagctt gatgttgaac ttgaagcggc cgacgcgcga aaagtcgtac ttcttgggat
     3601 cgaagaacat cccctcgaag aggtt
//
LOCUS       DASWAV010000187         5899 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U2665
            k141_491295, whole genome shotgun sequence.
ACCESSION   DASWAV010000187 DASWAV010000000
VERSION     DASWAV010000187.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960316
            Sequence Read Archive: SRR10260007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 5899)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5899)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:47:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,929
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5899
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_491295"
                     /isolate="SMAG_U2665"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.36444 N 132.31889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..744)
                     /locus_tag="VF958_03745"
     CDS             complement(<1..744)
                     /locus_tag="VF958_03745"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX9148253.1"
                     /translation="MRVFDCHVHVQPWEQFLPSSLDLMTRGRSDVERLERVTQDAAEL
                     LRLMDEQAIERAALVNYVAPEVIGFDESVNEWVSRYVAGHEDRLVAVGSVHPRRSKDA
                     RGDAARLFEELGIRMLKLHPPHQLFAANDYLSGNDALAAIYRTAEEHGRPVMIHTGTS
                     VFPGARNRFADPMAVDDVAVDFPRLPIVLAHAGRPLYMTTAVFLARRHRNVHLDLSGI
                     PPRKLLEYIPRLEEVSDRCLWGTDFPSPGI"
     gene            complement(772..1539)
                     /locus_tag="VF958_03750"
     CDS             complement(772..1539)
                     /locus_tag="VF958_03750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9148254.1"
                     /translation="MRGLVLALALSTMPVPLHAQVLPNLEPERPLAIEDAIPIPFRAL
                     SIGLDWTYNFRDSALDDAGPGISINYGALRDLEVGVAQRYVTRPGRNALRGISSGDVE
                     LHALYGLVDENGHTPALAARLGLVFPTGLDSRGTDLELGAIGTRSFERLRTHASFRWT
                     RLGDILPGERADRLEGGLAVDFLANSAGRTDTLILAGVTVRSSLVVNENATVDVEAGV
                     RQRLGVQTVVFGEIGSQITGRSDRTQLRLRFGISHFF"
     gene            complement(1577..2461)
                     /locus_tag="VF958_03755"
     CDS             complement(1577..2461)
                     /locus_tag="VF958_03755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9148255.1"
                     /translation="MRRFSGAARVAVLCAVAITAARTSASSQEPFRPKGRYNNSVHRF
                     FPDLDCRFNAVRYGRWRALEIAWRVGVNPELDREFSSFLLKLLSDPPRFAPEAEFIAP
                     RFAREASSVFHALHWGQVFEQQVSDTLASADATPELTAERLQRALEVYRREQWALLEP
                     KDEAQQPEALQLAPMSARILLSGTRLFVLAAEGLAAADFGQQRWKVRDTIAEFDSSLD
                     RDTTQEQATYRVSAPTVSDRYPVIAATLDRLARFRTEVYSALATGGASGEARKERNAR
                     LTAVAQRYGIPFRGIGAE"
     gene            complement(2493..3803)
                     /locus_tag="VF958_03760"
     CDS             complement(2493..3803)
                     /locus_tag="VF958_03760"
                     /inference="COORDINATES: protein motif:HMM:NF017760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX9148256.1"
                     /translation="MTDKEPLANGAAHPAFLKPAGPNLGVAPPRGAISPRRGMFSSLS
                     HRPFRRYWIGLFLSNIGTWMQVVAQGWLVLRLSDSAFMLGLVGFLGSIPTLLLAPLAG
                     VAADRLDRRKLLLMTQSTSIGCALLLAAATGFRFVSVPLVGAIAVINGLSNAFTMPSH
                     QSLFLDLVGREDLLNAISLNSMQFNLSRVIGPMIAGLTIAAFGETGCFVLNAVSYLAV
                     IAALAALPSMRRRPTPSRGAWIDLRIGLRFARRRPLIVSLLAIAAALAVFGTPAVTLA
                     PLFARRLLHVGPQGLGGMLSAVGLGAAASALALASRGDFRNKGRAVMLWALGFAASLL
                     GLALSRQYLVSLACLAILGASMSSSASLINTVLQTTAPDRLRGRVISLYALAWLGLVP
                     VGNLQAGAVAERFGAAASLFVGCAGILLTLLIVRIVKPVPRGAS"
     gene            complement(3800..5185)
                     /locus_tag="VF958_03765"
     CDS             complement(3800..5185)
                     /locus_tag="VF958_03765"
                     /inference="COORDINATES: protein motif:HMM:NF012686.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9148257.1"
                     /translation="MQTAVLLLLGVYFVAVLATGLAWRRRAGRDEVSYFVADRSLSTL
                     WGFLGLASLTTGGSTTIVLASFVYEHGISGLWLDLAGAFGLLLLGLLLAGRVRREGAV
                     TLPEIVARYYGNSAGRAAAGLVLVSEIVWFALLVTATQTVLTAALSVPALPALVASTA
                     VFVAYTALGGQFAVARTDLVQYGVMLAAIPGAALYFALKGAHGLGGLPSGTWSFPVSR
                     GVGPGDIFSLLVLVGLPHLVGSDVYAKVLSCRDERTARRAALLAAASKVVFGLAVAAI
                     ALAARKALPPVTPGEALPAAVLGFAPPAAAALVLVGLVATMQTSADVVLLSACAVSVR
                     DLAPAFLGRSPGVAAARALAPVYGALGLLVALALKSNVLETLKLGYSIFAAGIILPVL
                     VALAPGRPRVPPSGAIVAMIAGGGVAAAGRFAPGLTRGVDPVIAGTAVNLLVLAVSFA
                     VSRFPFSVSRA"
     gene            complement(5192..>5899)
                     /locus_tag="VF958_03770"
     CDS             complement(5192..>5899)
                     /locus_tag="VF958_03770"
                     /inference="COORDINATES: protein motif:HMM:NF013696.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004180 - carboxypeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HEX9148258.1"
                     /translation="AAIGLHLWNQLPVGRIGINRRALMAAVDEFEIHVTGRGGHGAAP
                     HETADPIVAAARIVEALQTIVSREVSPLDSAVVTIGTIHGGDAFNVIPSSVRLTGTAR
                     WFAETTGAAMPGKIERIATHVAAAAGVTARLEYKRVNRPVVNDPRITEIVIQAAARLL
                     GEQCVETETRTLGGEDMSVYLDAVPGCFFFVGSAPEGPHRPHHSPGFAIDERALAVAT
                     VLLEAAARDVSKALQTS"
BASE COUNT         1085 a         1937 c         2128 g          749 t
ORIGIN      
        1 gattccgggg ctcgggaaat cggtccccca caggcatcgg tccgagactt cttcgaggcg
       61 ggggatgtac tcgaggagct tgcgcggggg aatgcccgag aggtcgaggt ggacgttgcg
      121 atgccgccgc gcgaggaaga ctgccgtcgt catgtagagg gggcggcccg cgtgggcgag
      181 gacgatcggc aggcgcggga aatccactgc gacgtcgtcc accgccatcg ggtccgcgaa
      241 gcggttgcga gctcccggaa agacggacgt ccccgtgtgg atcatcacgg gccggccgtg
      301 ctcctccgcg gtccggtaga tcgcggccag cgcgtcgttt ccggagaggt agtcgttcgc
      361 ggcaaagagc tggtggggtg ggtgcagctt cagcatccgg atgccgagct cttcgaagag
      421 gcgcgcggca tcgccgcggg catccttcga ccggcggggg tggaccgagc ccacggcgac
      481 gaggcggtcc tcgtggcccg cgacgtagcg ggaaacccac tcgttcaccg actcgtcgaa
      541 cccgatcact tcgggagcga cgtagttgac gagcgccgcc cgctcgatcg cctgttcgtc
      601 catgagacgg agcagctcgg cggcatcttg cgtcacgcgc tccagccgct cgacgtcgct
      661 tcgtccacgc gtcatcaggt ccagactcga aggaagaaac tgctcccacg gctggacgtg
      721 gacgtgacag tcgaagacgc gcaagctcga gccggaccct ggcacgtcgt ctcagaagaa
      781 gtgagaaatg ccgaaacgca aacggagctg cgtccgatcg gacctccccg tgatctgcga
      841 gccgatctct ccgaagacga ccgtctgaac cccgagtcgc tggcggacgc ccgcctcgac
      901 gtccacggtc gcattctcgt tgaccacgag gctcgagcgc accgtcacac ccgccagaat
      961 cagcgtatct gtccgtccgg cgctgtttgc gaggaaatcg accgcgagcc ctccctcgag
     1021 ccgatctgcc cgctcgcccg gaaggatgtc gcccagccgg gtccagcgga agctcgcgtg
     1081 ggtgcggagc ctctcgaacg agcgggtgcc gatcgctccg agctccagat cggtcccgcg
     1141 ggagtcgaga cccgtcggga agacgagacc gagacgcgcg gcaagagcgg gggtatgccc
     1201 attctcgtcc acgagtccgt agagagcgtg gagctcgacg tctcccgacg agatcccgcg
     1261 caaggcattt ctgccagggc gggtgacata gcgctgcgcg acgccgacct cgaggtcccg
     1321 aagggcgccg tagttgatcg aaattcccgg ccccgcgtcg tccagggctg agtctcggaa
     1381 gttgtaggtc cagtcgaggc cgatggagag agcccggaaa ggaatcggaa tcgcgtcttc
     1441 gattgcgagc ggtcgctccg gctcgaggtt cggaaggacc tgggcgtgga ggggaacggg
     1501 catggtcgac aacgccaggg cgagtaccag ccccctcacc ccgaccctct cccggaggca
     1561 cagggaaaac gcgcggctat tccgcaccga tgccccggaa tggaatcccg tagcgctgcg
     1621 cgaccgccgt gagtcgcgcg ttgcgttcct tgcgcgcctc cccgctcgcg ccccccgtgg
     1681 cgagcgcgct gtagacctcg gtgcgaaagc gggcgagccg gtcgagggtc gctgcgatca
     1741 cggggtagcg gtccgacacc gtcggcgcgg agactcgata ggtcgcctgt tcctgcgtcg
     1801 tgtcccgatc gagagacgag tcgaactcgg cgatcgtgtc gcggactttc cagcgctgct
     1861 gtccaaagtc ggccgcggcg agaccttccg ccgcgagcac gaagagacga gtccccgaga
     1921 gaaggatccg ggcggacatc ggcgcgagtt gaagcgcttc gggctgttgc gcctcgtcct
     1981 tcggctccaa cagcgcccac tgctcgcgcc ggtagacctc gagtgcgcgc tggaggcgtt
     2041 ccgcagtgag ctcgggggtc gcgtccgcgg aggcgagcgt gtcgctcacc tgctgttcga
     2101 agacctgtcc ccagtgaagc gcgtggaaga cgctggaagc ctcgcgggcg aaacgcggag
     2161 cgatgaactc cgcctcgggc gcgaagcgtg ggggatccga gaggagcttc aggaggaagg
     2221 aggagaactc gcgatcgagc tccggattga cgccgaccct ccaggcgatc tcgagcgcgc
     2281 gccaccggcc gtagcgcacc gcgttgaagc ggcagtcgag atcggggaag aagcgatgga
     2341 ccgagttgtt gtagcggcct ttcgggcgga acggctcctg ggagctcgcc gacgtccgcg
     2401 cggcagtgat cgcgaccgcg caaaggaccg cgacgcgtgc cgcgccggaa aaccgcctca
     2461 tcggggtttg ggaatatagc ccatcgggcg gctcacgacg ctccccgcgg cacgggcttc
     2521 acgattcgaa cgatcaggag agtcagaagt atccccgcgc agcccacgaa gagcgacgcg
     2581 gcagctccga accgttcggc gaccgcaccg gcctggaggt tcccgaccgg gacgagcccg
     2641 agccaggcga gcgcatacag cgagatcacg cggcctcgca ggcggtcggg agcggtcgtc
     2701 tgcaacacgg tgttgatgag cgacgccgag gaagacatcg aagcgcccag tatcgcgagg
     2761 cacgccagcg agacgaggta ctggcgggag agagcgaggc cgaggaggga cgccgcgaag
     2821 ccgagagccc acaacatgac cgcgcgcccc ttgttccgga agtcgccgcg ggaggcgaga
     2881 gccagcgcgg acgccgccgc gccgagcccg acggccgaga gcatgccgcc gagcccctgg
     2941 ggaccgacgt gcagcagccg ccgcgcgaaa agcggcgcca gcgtgaccgc aggcgtgccg
     3001 aataccgcga gcgccgcggc gatggcgagg agagacacga tcagcggacg ccggcgagcg
     3061 aatcggagac cgatgcgcaa gtcgatccag gcgccccggc tcggcgtggg ccgccggcgc
     3121 atcgaaggga gggcggcgag cgccgcaatc accgcgaggt aggagaccgc gttcaggacg
     3181 aagcagcccg tctcaccgaa cgcggcgatc gtcagccctg cgatcatcgg cccgatcacg
     3241 cgagagaggt tgaactgcat cgagttgagc gagatcgcat tcaggaggtc ctcgcgtccg
     3301 accaggtcga ggaagagcga ctggtgcgag ggcatcgtga atgcgttcga gagcccgttg
     3361 atgacggcga tcgccccgac gaggggcacc gagacgaaac ggaatcccgt cgcggcggcg
     3421 aggaggagag cgcatccaat cgaagtgctc tgagtcatca gcaggagctt gcggcggtcc
     3481 aggcgatccg ccgcgacccc cgcgagcggg gcgagaagca gcgttggaat cgagcccagg
     3541 aacccgacca gccccagcat gaacgcggag tccgagagtc tcagaacgag ccagccctgc
     3601 gcgacgacct gcatccacgt tccgatgttc gaaaggaaga gcccgatcca gtagcgccga
     3661 aacggacggt gagagagcga cgagaacatg ccccgccgcg gggagatcgc gccgcgcggc
     3721 ggcgcgacgc cgaggttggg cccggcgggc ttgaggaaag ccggatgcgc ggcgccgttc
     3781 gcgagcggct ctttgtcggt catgcgcggg aaacggaaaa cggaaaacgg gaaacggcaa
     3841 acgacacggc gaggacgagg aggttgacgg cggttcccgc gatgaccgga tcgacgccgc
     3901 gagtgagtcc gggagcgaag cgaccggcgg ccgcaacccc gccgccggcg atcatcgcca
     3961 cgatcgcgcc gctcggagga acgcggggtc tcccgggcgc gagcgcgacc agcaccggca
     4021 ggatgattcc ggctgcgaag atcgagtaac cgagcttcaa cgtttcgagg acgttcgact
     4081 tgagcgcgag agcgaccagg agaccgagcg ccccgtagac cggcgcgagc gcgcgcgctg
     4141 ccgcgactcc gggactgcga cccagaaaag cgggcgcgag atctcgcacg ctgaccgcgc
     4201 aggccgagag aagaacgacg tcggcggagg tctgcatcgt cgcgacgagt cccacgagca
     4261 ccagagccgc ggcggcgggt ggagcgaacc cgaggaccgc ggccggcagg gcctcgcccg
     4321 gcgtgacggg cggaagcgcc ttgcgcgcgg cgagcgcgat cgccgcgacc gccagcccga
     4381 agacgacctt cgacgcggcg gcgaggagcg ccgcgcgccg tgccgtgcgc tcatcccgac
     4441 acgagagaac tttcgcgtag acgtcgctgc cgacgagatg aggaagcccg acgaggacga
     4501 gcagggagaa aatgtcgccg ggaccgacgc cgcgcgaaac cgggaagctc caggtcccgc
     4561 tcggaagccc gccgagtccg tgcgcgcctt tcaatgcgaa gtagagggcc gcgcccggga
     4621 tcgccgcgag catcacgccg tactggacga ggtccgtccg cgccaccgcg aactgacctc
     4681 cgagcgccgt gtacgcgacg aagaccgccg ttgacgcgac gagagccggg agcgcgggaa
     4741 cggaaagagc ggcggtcagg acggtctgcg tcgcggtgac gagaagcgcg aaccagacga
     4801 tctccgaaac gaggacgagc cccgccgccg cgcgcccggc ggagttcccg tagtagcgcg
     4861 cgacgatctc gggcagcgtc accgccccct cccgccggac ccggcccgcg aggaggagtc
     4921 cgaggaggag gagcccgaac gctcccgcga ggtcgagcca caggccggag atcccgtgtt
     4981 cgtaaacgaa cgacgcgagc acgatcgtcg tggaaccacc ggtcgtgagg gacgcgagcc
     5041 cgagaaagcc ccagagcgtc gacaggctgc ggtcggcgac gaagtacgaa acctcgtcgc
     5101 gccccgcgcg gcgccgccag gcgagcccgg tggcgagaac ggcgacgaag tagacgccga
     5161 ggaggaggag aacggcggtc tgcacgcggg actagcttgt ctgcagagcc ttcgagacat
     5221 cccgcgcggc ggcttcgaga aggaccgttg cgaccgcgag cgcgcgctcg tcgatcgcga
     5281 agcccggaga gtggtgcggc cggtgcggcc cctccggcgc ggagccgacg aagaagaagc
     5341 agccgggtac cgcgtccagg tacaccgaca tgtcctcgcc gccgagcgtt cgcgtctccg
     5401 tttccacgca ttgctcgccg agcaggcgcg ccgcggcctg gatgacgatc tcggtgatcc
     5461 ggggatcgtt gacgacgggc cggttgacgc gcttgtattc gagccgcgcg gtgacgcccg
     5521 cagccgccgc gacgtgggtg gcgatcctct cgatcttccc gggcatcgcc gcgcccgtcg
     5581 tttcggcgaa ccagcgagcg gtcccggtta accgcacgct cgacggaatg acgttgaacg
     5641 catccccgcc gtgaatcgtc ccgatcgtga cgaccgcgga gtcgagcggc gacacctccc
     5701 gcgaaacgat cgtctggagt gcttcgacga tcctcgccgc cgcgacgatc ggatcggcgg
     5761 tctcgtgcgg cgccgcaccg tggcctccgc gaccggtcac gtgaatctcg aactcgtcga
     5821 cggcggccat cagcgcgcgc cggttgattc cgatcctgcc gaccgggagc tggttccaga
     5881 ggtgcagccc gatcgccgc
//
LOCUS       DASWAV010000188         2306 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U2665
            k141_450668, whole genome shotgun sequence.
ACCESSION   DASWAV010000188 DASWAV010000000
VERSION     DASWAV010000188.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960316
            Sequence Read Archive: SRR10260007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2306)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2306)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:47:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,929
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2306
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_450668"
                     /isolate="SMAG_U2665"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.36444 N 132.31889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..230
                     /locus_tag="VF958_03775"
     CDS             <1..230
                     /locus_tag="VF958_03775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9148259.1"
                     /translation="AANLTIVAPPTSGYVTLFPGSTPIPLFSSINFRPGLVRANNVIA
                     PLGPSGTLGIFCSTDPSAMVDFIVDVSGYFE"
     gene            283..1767
                     /locus_tag="VF958_03780"
     CDS             283..1767
                     /locus_tag="VF958_03780"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HEX9148260.1"
                     /translation="MRRPRVLALAGAALLFAYALALFHNTSFAAGGSDSSGYLNAARL
                     LAHGNATEPIRGLARLGLSQDFAPVFVPLGYTQLERPGLMAPSYPPGLPLHMALAARI
                     GGWARAPFLVSPLAGIACLVLLYFLGRELRLPPAFAAAGCALLAFFPTFVFQTLQPMS
                     DVLATAWALAAVLAAYRSRSRGSVAWSAFAGLAFGIGVLVRPTNAVLLLPLLVAIPAR
                     PKAWLAFIAGGAPAAAFLLGYDAVLFGSPWATGYRTAFSEGMAWANFPERARHYGLWL
                     ARLLTPLVPVGWIALVADRRAPSRDRAVLLSWFGAFFLFYSFYAPYEAWWYTRFLLPG
                     VPALILGTLVVARDLLDLSTTPQSRWARPAAAALLALVLFVEVRFIDRNRVHKFYKGD
                     REYVVACAMARRRLPPEAIVASMQMSGALHFYTGTTYAMWNWLLPDRFAVLRVSTESR
                     GHRWYALLAPFEQEEVRKNLPGDWREIDRVGDVALWELPPAAGR"
     gene            complement(1775..>2306)
                     /locus_tag="VF958_03785"
     CDS             complement(1775..>2306)
                     /locus_tag="VF958_03785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9148261.1"
                     /translation="IANDVLHHITGLEPLYTRIHDALAPGGRLVFNEYVGPNRFQYSD
                     AHLETVNRYLRLLPNRLRWDPIGGRLLWKRERVDPKQLVIDDPTEAVRSEDVLPLARR
                     FFKAEKEYVYGGGLLNPLLFGIVTNFRAGDPWDDMLMSRLCAAEQRLTEAGEIEPDFR
                     IFVGSRRDDRSDSDSG"
BASE COUNT          285 a          843 c          737 g          441 t
ORIGIN      
        1 tcgccgcgaa cctgaccatc gtggctcctc cgaccagcgg ctacgtgacg ctctttccgg
       61 gcagcacgcc gattccgctt ttctcctcga tcaacttccg gcccggtctg gtgcgcgcga
      121 acaacgtcat cgcacccctt ggaccgtccg ggactctcgg gatcttctgc tcgacggacc
      181 cctcggcgat ggtcgacttc atcgtcgacg tcagcggcta cttcgagtag cgcgctgaac
      241 cggccgcccg cggaccacgc tccgccgcga taacctcgtc gcgtgcgaag gccgagagtg
      301 ctcgcgctcg ctggcgccgc cctgctgttc gcgtacgcgc tcgcgctctt ccacaacacg
      361 tcctttgcgg cgggcggatc ggacagctcg ggctacctga acgccgcgcg cctcttggcg
      421 cacggcaacg cgaccgagcc gataagaggt ctcgcgcggc tcggcctttc gcaggacttc
      481 gcgcccgtct tcgtgccgct cggatatacg cagctcgagc ggccgggcct catggccccg
      541 agctacccgc cgggattgcc gctccacatg gcgctcgccg cgcggatcgg cggatgggcg
      601 agggcgcctt tcctcgtcag cccgctcgcc ggcatcgcct gcctcgttct cctgtatttc
      661 ctggggcgcg agctccggct gccgcccgcc tttgccgccg cggggtgtgc tctcctcgcc
      721 ttcttcccga cgttcgtgtt ccagacactg cagccgatga gcgacgtcct cgcgaccgcg
      781 tgggcgcttg cggccgttct tgccgcttac cggtcccggt cccgcggcag cgttgcctgg
      841 tcggccttcg ccgggctcgc gttcgggatc ggcgttctcg tgcggccgac gaatgccgtt
      901 ctcctcctgc cgctcctcgt cgcgattccg gcgcggccga aggcgtggct ggcgttcatc
      961 gccggaggag cgcccgcggc ggcctttctc ctcgggtacg acgcggtgtt gttcggcagc
     1021 ccctgggcca ccggttatcg aaccgcgttc tccgagggga tggcctgggc gaactttccc
     1081 gagcgggcgc ggcactacgg tctctggctc gcgcgcctac tgaccccgct cgttcccgtc
     1141 ggctggattg ccctcgtcgc cgaccgccgc gctccctcgc gcgaccgcgc ggttctcctc
     1201 tcgtggttcg gagcgttctt cctcttctac tccttctacg ctccctacga ggcctggtgg
     1261 tacacgcggt ttctcctccc cggcgtcccc gcactcattc tcggcaccct cgtcgtcgcg
     1321 agagaccttc tcgatctctc gacgaccccg cagagccggt gggcgcggcc cgctgccgct
     1381 gctctcctcg cgctcgtcct cttcgtggag gtccggttca tcgaccgcaa ccgcgtgcac
     1441 aagttctaca agggggaccg cgagtacgtc gtcgcctgcg cgatggcgcg ccggcggcta
     1501 ccgccggagg cgatcgtcgc ttccatgcag atgagcggag cccttcactt ctataccggc
     1561 acgacctacg cgatgtggaa ctggctcctg cccgatcgat tcgcggtcct ccgcgtgtcc
     1621 acggagagcc gcggccaccg ctggtacgcg ctgctcgctc ccttcgaaca ggaagaagtg
     1681 cgcaagaacc tgcccggcga ctggcgcgaa atcgatcgtg tgggggacgt cgccctgtgg
     1741 gagctcccgc ctgcagccgg ccgctgaggc tgcgtcagcc ggagtcggag tccgacctgt
     1801 cgtcgcgccg cgatcccacg aagatccgga agtccggctc gatctctccc gcttcggtca
     1861 gccgctgctc cgccgcgcac aggcgcgaca tcagcatgtc gtcccacgga tctccggcgc
     1921 ggaagttcgt cacgattccg aagaggagcg ggttcagcaa ccctccgccg tagacgtact
     1981 ccttctcggc cttgaagaac cgtcgcgcga gcggcagcac gtcttcgctc cggacggcct
     2041 ccgtggggtc gtcgatgacg agctgcttcg gatcgacgcg ttcccgcttc cagagcagcc
     2101 gtccgccgat cgggtcccag cggaggcggt tcgggagcag gcgaagatac cggttcacgg
     2161 tctccaggtg cgcgtccgag tactggaagc gattcggccc cacgtactcg ttgaagacga
     2221 gccgtccgcc gggagcgagg gcgtcgtgaa tccgtgtgta gagcggctcg agtccggtga
     2281 tgtggtggag gacgtcgttc gcgatg
//
LOCUS       DASWAV010000189         5460 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U2665
            k141_529695, whole genome shotgun sequence.
ACCESSION   DASWAV010000189 DASWAV010000000
VERSION     DASWAV010000189.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960316
            Sequence Read Archive: SRR10260007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 5460)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5460)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:47:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,929
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5460
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_529695"
                     /isolate="SMAG_U2665"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.36444 N 132.31889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1290)
                     /locus_tag="VF958_03790"
     CDS             complement(<1..1290)
                     /locus_tag="VF958_03790"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEX9148262.1"
                     /translation="MSLAAGSRLGPYEIVSPLGAGGMGEVYRARDTKLDREVAIKVLP
                     EKLAADSDSLARFEREAKAVAALSHPNILGIYDLGRDGGVAYAAMELLRGEMLRERLE
                     EGALPQRKALEYGLQVAHGLAAAHEKGIVHRDLKPENVFVTPEGRLKILDFGLAKVSA
                     VQSDETRSPTVAATEPGTVMGTVGYMSPEQVRGRPADHRSDIFSLGAILYEMFSGERA
                     FRGESAAETMAAIAQKDPPQLAESAGRFPPSIERILRHCLEKRPEERFDTAHDLAFAL
                     ETAMGTPSATSQVVPAIVSKRRWLTPVLGVLALLAALGAGFLAAGRMRAPTAPSFRQI
                     TYHRGYIQSARFAPDGQTVVYGSTRRDEPLRIYSTRLDSIESRSLELPPADVVGMSKN
                     GQMAVLLNRRHAGTWITIGTLARSDLAGGAPHEILENV"
     gene            complement(1298..1693)
                     /locus_tag="VF958_03795"
     CDS             complement(1298..1693)
                     /locus_tag="VF958_03795"
                     /inference="COORDINATES: protein motif:HMM:NF013970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HEX9148263.1"
                     /translation="MILLDTHVLLWLAAEPARLSRAAASAIRRAVRSGGIAIASISLW
                     EIAALFARGRLRSRGTTEASLEAILKATGVSLREITPTIASLATQFPADFSSDPADRL
                     IAATARAEGLTLVTRDEQIRSSALVRTVW"
     gene            complement(1690..1923)
                     /locus_tag="VF958_03800"
     CDS             complement(1690..1923)
                     /locus_tag="VF958_03800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013034358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HEX9148264.1"
                     /translation="MRTIPAARFKAGCLKIMDDVKARREPVLITKKGKPVARLVPVDE
                     PAGDVFGCLAGELEISGDILSPVITPSAWKGSR"
     gene            complement(1990..3009)
                     /locus_tag="VF958_03805"
     CDS             complement(1990..3009)
                     /locus_tag="VF958_03805"
                     /inference="COORDINATES: protein motif:HMM:NF016817.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M14 family metallopeptidase"
                     /protein_id="HEX9148265.1"
                     /translation="MALAAPAAETPLLTVGTAAAGPGQKVYGAIEVPAGVDPGTRIDV
                     AVVRGAKPGPVLAVVSGAHGTEYASIVAVSRLIQALDPAAISGTVILVPLVNVASFEQ
                     KVPHVNPVDGKSMNRFYPGKADGTQTERASFAITREVVDKCDHLIDLHGGDLDEDLRP
                     YSYWTKTGNAVQDAVSREMVLAFGLDHVIVSTDRPKDPQASRYLENTATTRGKPSITV
                     EAGHAGTVEPDDVAALVDGTLSVMRYLKMLAGAPTRVEHPVWIDSVKTIASGETGLFY
                     AVVRRGSYVAEGTRIGYVTDFVGRTIFEARAPAAGVVLYICSVPSMKKGDTVANVGVV
                     APSPP"
     gene            3113..3385
                     /locus_tag="VF958_03810"
     CDS             3113..3385
                     /locus_tag="VF958_03810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018928052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4242 domain-containing protein"
                     /protein_id="HEX9148266.1"
                     /translation="MPKFVIEREIPGLGNLTDEEVKAVARKSNEILTSLGPEIQWLHS
                     YVTGDKMYCVYIAPDEELIREHARRGQFPANRISAVRRLVDPMTAV"
     gene            complement(3395..>5460)
                     /locus_tag="VF958_03815"
     CDS             complement(3395..>5460)
                     /locus_tag="VF958_03815"
                     /inference="COORDINATES: protein motif:HMM:NF037660.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX9148267.1"
                     /translation="VAEEISGRFPGGATFVPLDSARDADQLIAAICRAVGVRETTEQT
                     LLDSLKEHLAPTSRAPMLLVLDNFEQLLGVAPTVAELIAAGPGLKVVVTSRSPLHVYG
                     EHEFPVPPLALPEAGALPPLDRLTEYEAVALFLQRAQAVKPNFALTKENAPAIVEICA
                     RLDGLPLALELAAARVKLLSPAAMQSRLEKRLQLLTGGGIDLPARQRTLRGAIEWSYD
                     LLNEDEQKLFRRLSVFVDGCTLEAVEAVCNAKEDLVVDSLDGMASMVDKSLMRQSETT
                     GDEPRFVMLETIREYALEQLAASGDEPITRRAHAAYFLVLAEEVGSARAATLVDSAQW
                     LDRCEREHGNFLAALEWVCQNDQGQWGLRLGGALFGFWERREYISEGRDWLAKLLALP
                     SAAGRDRVRARAVFAAGVLAGVQRETELANTLLTESLEINREAGDKWAAAVSLNALAV
                     TALDRRDVAGARSLSEQNLEVWRELGDRAAVARSLSNLASVVKEQGDFSRARSLYDEA
                     LATFREIGDATAAARVLNKQGDVAREQGSPEEAQALYEQSLETFREFGDRWGTALALA
                     DLGNLARERGDLSSARSLYRDSLRIFQELDYKRGVAQVLESFACAAADQGEAKKALVL
                     AGAAAALRHALGTPLRSSEQAVLETSLEPARKSLAGSEGAAAWMEGWTTPLESAVEAV
                     LSAEA"
BASE COUNT          913 a         1826 c         1826 g          895 t
ORIGIN      
        1 gacgttctcg aggatctcgt gcggggctcc gccagcgagg tccgagcgcg cgagggtccc
       61 gatcgtgatc caggtgccgg cgtgccggcg gttcaacagc acggccatct ggccgttctt
      121 cgacatgccg acgacgtcgg ccggcgggag ttcgagcgag cgcgattcga tgctatcgag
      181 ccgcgttgaa tagatccgaa gaggctcgtc tctccgcgtc gaaccgtaga cgacggtctg
      241 cccgtccggg gcgaaacggg cggattggat gtaaccgcgg tggtaggtga tctggcggaa
      301 ggaaggagcc gtcggcgcgc gcatccgccc cgctgccagg aaaccggcgc cgagcgcggc
      361 aaggagagcc agcactccga ggaccggagt cagccaccgt cttttcgaga cgatcgctgg
      421 cacaacctgc gacgtcgcgc tcggtgtgcc catcgccgtc tcgagcgcga acgcgaggtc
      481 gtgcgccgtg tcgaagcgtt cctccggcct cttctccagg cagtggcgca ggatgcgctc
      541 gatagatggc ggaaaccgcc ccgccgattc ggcgagctgt ggcgggtcct tctgcgcgat
      601 cgctgccatc gtctccgcag cggactcgcc acggaaagcg cgctcgccgg agaacatctc
      661 gtacaggatc gcgccgagcg aaaagatgtc ggagcggtgg tccgccggcc tgccccggac
      721 ctgttccggc gacatgtagc cgacggtccc catgaccgtg ccgggctcgg tggccgcaac
      781 cgtcggcgat cgcgtctcgt ccgactgcac cgccgaaacc ttggcgaggc cgaaatcgag
      841 gatcttcagg cgcccttccg gtgtgacgaa gacgttctcg ggcttcagat cgcggtggac
      901 gattcccttc tcgtgcgcgg ccgcgagccc gtgcgcgacc tgcaacccgt actcgagcgc
      961 cttcctctgc gggagagcgc cttcttcgag ccgttcccgg agcatctctc cccggagcag
     1021 ctccatcgcg gcgtacgcaa caccgccgtc gcgtccgagg tcgtagatcc ccaggatgtt
     1081 gggatgcgaa agcgccgcga cagccttcgc ttcacgctcg aagcgcgcga gggagtccga
     1141 atcggcagcg agcttctccg gcaggacttt gatcgcgacc tcccggtcga gtttcgtgtc
     1201 ccgcgcccgg tagacctccc ccatcccgcc cgcccccaag ggcgacacga tctcgtaggg
     1261 gcccagacgc gaaccggcgg caagactcac gattccgtca ccagaccgtc cgaacgagcg
     1321 cgctcgagcg gatctgctca tcgcgcgtga cgagggtaag tccttcggcc cgggcggttg
     1381 cagcgatgag ccggtccgcc gggtcgctcg agaaatcggc gggaaattgc gtcgccaggg
     1441 aggcgatcgt cggcgtgatc tctcggagcg agacgcctgt ggccttcagg atggcttcca
     1501 gagacgcttc cgtggtcccg cgggaccgca gccgtcctct cgcgaagagc gcggcgatct
     1561 cccagaggct gatcgaagcg atcgcaatgc cgccggaacg aactgcccgg cgaatcgccg
     1621 acgccgccgc cctcgaaagg cgtgcgggct ccgctgcgag ccacagaagc acgtgcgtat
     1681 cgaggagaat caccgtgagc ccttccaggc ggacggggtg atgacgggcg acagtatgtc
     1741 cccggagatc tcgagctctc cggccagaca accgaagacg tcgcccgcgg gctcgtccac
     1801 gggtaccagc cgtgcgacag gttttccctt cttcgtgatc agtacgggct cgcggcgagc
     1861 cttcacgtcg tccatgatct tcaagcagcc ggccttgaat ctcgcagcgg gaatggttct
     1921 catagtcatc tccagaagac catggttata acatactggt cagaaagtgg acgggaccgg
     1981 cgaggggcct cacggggggg acggggcgac gacgccgacg tttgcaacgg tgtcgccctt
     2041 cttcatcgag gggacggagc agatgtagag gacgacaccc gccgccggcg cgcgcgcttc
     2101 gaagatggtg cgaccgacga agtcggtcac gtagccgatc ctcgtcccct ccgcgacgta
     2161 gctcccgcgc cggacgaccg catagaagag tcccgtctca ccgctcgcga tcgtcttcac
     2221 cgagtcgatc cagaccggat gctcgacgcg cgtcggagcc cccgccagca tcttcaggta
     2281 tcgcatgacc gagagggttc cgtcgacgag cgccgcgaca tcgtccggtt cgacggtgcc
     2341 ggcgtggccg gcctcgaccg tgatcgacgg cttgccgcgc gtcgtcgccg tattctcgag
     2401 gtagcgggat gcctgaggat ccttcggtcg gtccgtcgaa acgatgacgt ggtcgagtcc
     2461 gaaggcgagc accatttcgc gtgagaccgc gtcctggacg gcgtttcccg tcttcgtcca
     2521 gtagctgtag ggtcgcaagt cttcatcgag gtcgccgccg tgcaggtcga tcaagtggtc
     2581 gcacttgtcg acgacctcgc gtgtgatcgc gaacgaagca cgctccgtct gggtgccgtc
     2641 tgccttcccc gggtagaagc gattcatgct cttgccgtcg accgggttca cgtgagggac
     2701 cttctgctcg aaggaggcga cgttgacgag cgggacgaga atcacggtcc cggagatggc
     2761 ggcggggtcg agagcctgga tcagtcgcga gacggcaacg atcgacgcgt actcggtccc
     2821 gtgcgctccg gagacgacgg ccaggaccgg tcccggcttc gctccacgga cgaccgcgac
     2881 gtcgatccgc gtgccgggat cgactcccgc cggcacctca attgcgccgt agaccttctg
     2941 ccccggacca gccgcagccg ttcccaccgt gaggaggggt gtctccgcag ccggagcggc
     3001 gagcgccacc gtgagcgtca gagtcgcgac cgaaagaaag gtcgtaatga agggattcta
     3061 acgagtcgac acccgcgccg ggactcggct agcattcgga gcggaggcgt aaatgccgaa
     3121 gttcgtgatc gaacgggaaa tcccgggtct cggaaacctg acggacgaag aggtgaaggc
     3181 ggtagcccgg aagtcgaacg agatcctgac gtcgctcgga ccggagatcc agtggcttca
     3241 cagctacgtc acgggcgaca agatgtactg tgtctacatc gcgcccgacg aggagctgat
     3301 ccgcgagcac gctcgccggg gtcagtttcc ggcgaaccgc atttcggccg tgaggagact
     3361 ggtcgatccg atgaccgcgg tctgagagtg agggtcaggc ctccgcgctg agaaccgctt
     3421 cgaccgcgct ttcgagcggc gtcgtccaac cttccatcca tgcagccgcg ccctccgacc
     3481 cggcgaggct cttccgggcc ggttcgagac tcgtctcgag aaccgcctgc tcggaagacc
     3541 gcaacggcgt tcccagggcg tgccggagag cggcggcggc tcccgcgagg acgagagcct
     3601 tcttcgcctc gccctgatcc gcggccgcgc acgcgaaaga ctccaggacc tgcgcaacgc
     3661 cgcgcttgta gtcgagctcc tgaaagatcc gaagactgtc gcggtacaga gatcgcgccg
     3721 aggagaggtc cccgcgctcg cgggcgaggt ttccgaggtc ggcgagggcg agcgccgtgc
     3781 cccaccggtc gccgaattcg cgaaacgtct cgaggctctg ctcgtagagt gcctgtgcct
     3841 cctccgggct tccctgctcc cgcgccacgt ccccctgttt gttgagcacc cgcgcggccg
     3901 cagtcgcgtc gccgatttcc cggaaggtcg cgagcgcttc gtcgtagagc gaacgcgcgc
     3961 gggaaaagtc tccctgttcc ttgacgacgc tcgcgaggtt cgaaagcgac cgtgcgaccg
     4021 cggcgcgatc tccgagttcg cgccagacct cgaggttctg ctccgagagc gagcgcgccc
     4081 cggcaacgtc cctgcgatcg agggcggtta ctgcgagcgc gttcaacgag accgcggccg
     4141 cccatttgtc gcccgcctcc cggttgatct cgagactctc ggtcaggagg gtgttggcga
     4201 gctcggtttc ccgctggacg ccggccagga ccccggcagc aaaaacggcg cgtgctcgca
     4261 ccctgtcgcg tcccgcggcg ctcggaagcg cgaggagttt cgccagccag tccctcccct
     4321 cggagatgta ttcccggcgc tcccagaatc cgaacagcgc accgccgagc cgcaaccccc
     4381 actgcccctg atcgttctgg cacacccact cgagcgcggc gaggaagttc ccgtgctctc
     4441 gctcacatcg gtcgagccac tgtgcggagt cgacgagggt ggcggcgcgg gccgacccga
     4501 cttcttccgc gagaacgaga aagtatgcgg cgtgagcgcg ccgggtgatc ggctcgtcgc
     4561 cggaagcggc cagttgctcc agcgcgtact cgcgaatcgt ctccagcatg acgaagcggg
     4621 gctcgtcccc ggtcgtctcg ctctgacgca tcaggctctt gtccaccatg gacgccattc
     4681 cgtcgaggga atcgacgacc agatcttcct tcgcattgca gacggcttcg accgcttcga
     4741 gcgtgcagcc gtccacgaac accgacagac gccggaagag cttctgctcg tcctcgttca
     4801 gcaggtcgta gctccactcg atcgcgccgc gcaaggttcg ctgtcgcgcc ggcaggtcga
     4861 tcccgccgcc ggtcaggagc tgcaaccgct tctcgagtct cgattgcatg gccgccggcg
     4921 agaggagctt gacgcgcgcg gcagcgagct cgagggcgag gggcagaccg tccagacggg
     4981 cgcagatctc gacgatcgcc ggtgcgttct ccttcgtgag cgcgaagttc ggcttgaccg
     5041 cctgcgcccg ttgcaggaag agagcgaccg cttcgtactc ggtgagccgg tcgagcggcg
     5101 gaagggcccc cgcctcggga agcgcgagcg gcggcaccgg aaactcgtgc tctccgtaga
     5161 cgtgcagggg cgatcggctg gtgacgacga ctttgagtcc gggtccggca gcgatcaact
     5221 cggcgacggt cggagccacc ccgagaagct gctcgaagtt atccagcacg aggagcatcg
     5281 gcgcgcgtga ggtcggagcg aggtgttcct tcagcgagtc gaggagcgtc tgttccgtgg
     5341 tctctcgcac accgaccgcc cgacagatcg cggcgatcag ctggtcggca tcgcgggcgg
     5401 agtcgagcgg gacgaacgtc gctccgcccg gaaaccgccc cgagatctcc tcggcaacct
//
LOCUS       DASWAV010000190         5293 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U2665
            k141_10901, whole genome shotgun sequence.
ACCESSION   DASWAV010000190 DASWAV010000000
VERSION     DASWAV010000190.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960316
            Sequence Read Archive: SRR10260007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 5293)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5293)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:47:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,929
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5293
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_10901"
                     /isolate="SMAG_U2665"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.36444 N 132.31889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..351)
                     /locus_tag="VF958_03820"
     CDS             complement(<1..351)
                     /locus_tag="VF958_03820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HEX9148268.1"
                     /translation="MPVSTRAVILAGGEGQRLGVLTDRRAKPAVPFAGKYRIIDFTLS
                     NCVNSGIFDIMILTQYRPHSLIEHIGAGRPWDLDRGFTGGVHIYQPYRGRLDTDWYRG
                     TADAIYQNLPFVKRG"
     gene            complement(352..1809)
                     /locus_tag="VF958_03825"
     CDS             complement(352..1809)
                     /locus_tag="VF958_03825"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR02095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004373 - glycogen (starch) synthase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen/starch synthase"
                     /protein_id="HEX9148269.1"
                     /translation="MKILFLAAEAAPFVKVGGLGDVAGSLPSALAALGHDVRLALPGY
                     GAIDRARFGFHSRARFGVRSTGGPVAAELWEADAGRVPAYLVTGPPIPTDRRIYGGSI
                     EEDAPKFVFFSLAALAACRELRWKPDVVHANDWHTGAAVYSLANSGGADEFFRETATL
                     FTIHNLPYIGRNAGSALAQWGLPPTDALRLLPEWARDSLLGIGIATADVLSTVSPTYA
                     REIRTPEAGQGLDGVLRARGDRLHGVLNGIDVDVWDPASDPELRARFDVETIEKRKTN
                     RAALQEEAGLEADERALLLGMVSRLDSQKGFDIALPVLARWLDSGGQVVVLGSGDPAI
                     EHSVATLELQWQGRASARLRFDAPYSSLIYGGADAMLIPSKYEPCGLTQMIAMRYGAV
                     PIARRTGGLADTITDVSDEGGTGILFDGYDTGSLAHALERAVRVYAQPDRWIELQHRG
                     MTRDLSWNRSALEYEALYGEALRFRRGTTRPGEKD"
     gene            2054..3865
                     /locus_tag="VF958_03830"
     CDS             2054..3865
                     /locus_tag="VF958_03830"
                     /inference="COORDINATES: protein motif:HMM:NF017745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF885 domain-containing protein"
                     /protein_id="HEX9148270.1"
                     /translation="MRELSSARIVGALVFLVTLGVAIPNSPGPARSAAIAMASTRSSE
                     LLAKAERDYWDFLQKRSVALRLRMGLPIEELPDLSEERARADARYGASLLEKLRGLRA
                     HELSHEESLSLAMLGERARELADSRRDYWLAFPVTPYASPLAGVEEVFTTWRFDDPQD
                     LERYAALLTRYAAMVRQMEDKLREQARRGIRLPRPEAALVAETYRSEVAEPDASFLFV
                     TPDRLEKVPDQDRRPFTKRVEGLIESRVNPALESLAAYVSGDYAARAPRSVGLGQYAG
                     GKAYYRRLVKQYTTLDVTPEEVHRFGLAEIERLNRELDEVRVAVGFDGTLAEFRRQLK
                     SDPRFVPATSDAIGERLLEAQKRIGSKIPLFFGKMPSAPAGIRRLASTLEGAMTYGYY
                     QAPTVSDARGYYYYNGSKLGERSMLNAAALIYDELVPGHHFQVSLQQENSELPSFRRE
                     ARWAAYTEGWGEYASSLAAEMGMYDDPYDRAGRLMRDAYLSAGLVVDTGMNALGWSRA
                     RATAYLKENTLESDTQIAAEMLRYSCDVPGQALAYKIGAAKIRELREKASRELGRSFD
                     VRRFHDAVLGSGAMPLAVLERHIDWFIESQRTRLASR"
     gene            complement(4012..4590)
                     /locus_tag="VF958_03835"
     CDS             complement(4012..4590)
                     /locus_tag="VF958_03835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9148271.1"
                     /translation="MKTTLRIGALGGAALLLTLLACLSSAAPRTGGSARSSLTDLASM
                     LSGRFRGTTPGNDLRLDLSTVGASAGTRFDLFLTSSGRYRDTNVRRVGLLRLETQGRD
                     VHAVYVPHFDSTVTPLSRAATRFTQEELEAACNLYFHPSGDGYVGELAGATTCARALP
                     GAVGKWTVEVEPGGVRVRNVDSGETLRFAKTR"
     gene            4665..5279
                     /locus_tag="VF958_03840"
     CDS             4665..5279
                     /locus_tag="VF958_03840"
                     /inference="COORDINATES: protein motif:HMM:NF015147.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LEA type 2 family protein"
                     /protein_id="HEX9148272.1"
                     /translation="MNVRKSPPRGPRRFLTGLPAATALSLVLGTAGCSSVFERPELQF
                     RGIHVNSIGFDGASLEILVDVYNPNSYRLGLERLSYDLVIENVHWGLGSLTAPIGVEG
                     HDATMVRLPLDVSWSRLGDVGREALRTGTVNYGISGEMTVATSLGNIDVPYSKAGRFS
                     VLTGQESSDPGPIPPDPTCGFRQAIYSIETTTPFSKTTFFPTLL"
BASE COUNT          892 a         1746 c         1801 g          854 t
ORIGIN      
        1 gccacgcttg acgaacggga ggttctggta gatcgcgtcg gccgttccgc gataccagtc
       61 ggtgtcgagc cgcccccggt agggctgata gatgtgaact ccgccggtga agccgcggtc
      121 gaggtcccat ggacggcccg ccccgatgtg ctcgatcaaa gagtgggggc ggtactgcgt
      181 caggatcatg atgtcgaaga tcccggagtt gacgcagttc gacagcgtaa agtcgatgat
      241 ccggtacttt ccggcgaaag gcaccgccgg cttggcgcgg cgatcggtca ggactccgag
      301 gcgctgtccc tctccgcccg cgagaatgac cgctctcgtg ctgacgggca ttcagtcttt
      361 ctcaccgggt cgcgttgtgc cgcgccggaa gcggagtgcc tcgccgtaga gcgcctcgta
      421 ctccagggcg gagcgattcc acgagagatc cctcgtcatt ccccgatgct gcagctcgat
      481 ccatcggtca ggttgagcgt acacccggac ggcgcgttcg agcgcatggg cgagcgagcc
      541 ggtgtcgtac ccgtcgaaga gaatccccgt tccaccttca tcgctgacgt cggtgatcgt
      601 atcggcgagg ccgcccgtcc tccgcgcgat cggaacggct ccgtaacgca tcgcaatcat
      661 ctgggtgagc ccgcacggct cgtatttcga cggaatgagc atcgcgtcgg cgcccccgta
      721 aatgaggctg gaatacggcg cgtcgaagcg gagccgcgcg ctcgcgcgcc cttgccattg
      781 caactcgagg gtggcgaccg agtgctcgat cgccggatcg ccgctgccga ggacgacgac
      841 ctgtccgccg gaatcgagcc agcgcgcgag caccgggaga gcaatgtcga aacccttctg
      901 cgagtcgagg cgcgagacca tcccgaggag cagcgcacgc tcgtctgcct cgaggcctgc
      961 ctcctcctgg agcgcggcgc ggttcgtctt ccgcttctcg attgtctcga cgtcgaaacg
     1021 agcgcggagc tccgggtcgg acgcggggtc ccacacgtcg acgtcgatgc cgttcaagac
     1081 tccgtggagc cggtcgccgc gcgcacggag gacgccgtcg agcccctggc ccgcctccgg
     1141 tgtgcggatc tcgcgtgcgt acgtcgggct gaccgtggag agcacatcgg cggtggcgat
     1201 gccgatcccg agcaacgagt cgcgcgccca ctccggcagc agccgaagcg catcagtcgg
     1261 aggcagaccc cattgcgcga gggcgctccc ggcgttccgg ccgatgtagg gaagattgtg
     1321 gatcgtgaag agcgtcgcag tctcgcgaaa gaactcgtcg gctccaccgg agtttgcgag
     1381 ggaatagacc gcagcacccg tgtgccagtc gttggcgtga acgacgtccg gtttccaccg
     1441 aagctctcgg cacgcggcca gcgccgcgag agaaaagaaa acgaacttcg gagcgtcctc
     1501 ctcgatcgag ccgccgtaga tccgccggtc ggtcgggatg ggagggccgg tgacgagata
     1561 cgcggggacg cgcccggcat cggcctccca gagctcggcg gcaacggggc ctccggtcga
     1621 acgaaccccg aagcgggcgc gactgtggaa cccgaatcga gcgcggtcga tcgcgccgta
     1681 gccggggagg gccaggcgaa cgtcgtggcc gagggcggcg agcgcgctgg gcagagaacc
     1741 cgcgacgtca ccgagaccgc cgaccttgac gaagggagcg gcttccgcgg cgaggaacag
     1801 tatcttcaag cagccggatt atccgcggac gccaccccgg cgttacaatt attcaaggtt
     1861 tcatacatgc catggacgaa atcggccggc cacgcggggc ggggtgcagc cacagctctc
     1921 gggccgcgca cgaagtgttc ttttctggaa cgctcggggc ggaccccggg ttctcgaatg
     1981 gagcaatggt cgtccgagtc gagtccgggt gggcactttt cgcgcggatt gccgtgagag
     2041 caaggaagct cgcatgagag agctctcgag cgcgaggatc gtcggcgccc tggtctttct
     2101 cgtgacgctc ggcgtcgcga taccgaactc accgggcccg gcgaggtccg cggcgatcgc
     2161 gatggcttcg acgcgcagct cggagctcct cgcgaaggcc gagcgggact actgggactt
     2221 tctccagaag cgcagtgtgg cgctgcggct gcggatgggc ctcccgatcg aagagctccc
     2281 cgacctctcg gaggagcgcg cgcgcgccga cgcgcggtat ggggcatcac ttctcgagaa
     2341 gctgcgcggc ctgcgggcgc acgagctgtc tcacgaggaa tcgctctcgc tcgcgatgct
     2401 cggcgaacgg gcgcgggagc tcgccgattc gcggcgcgat tactggctcg cgtttccggt
     2461 gacgccgtac gcgtcgccgc tcgccggggt cgaggaagtc ttcacgacgt ggaggttcga
     2521 cgatccgcag gatctcgagc gttacgctgc gctcctcacc cgctacgctg cgatggtccg
     2581 gcagatggaa gacaagctcc gcgagcaggc gcgccgcggc atccgactgc cgagacccga
     2641 ggcggcgctg gtcgccgaga cttaccgcag cgaggtcgcg gagccggacg cgagcttcct
     2701 cttcgtgaca cccgatcgac tggagaaggt ccccgatcaa gaccgcaggc ctttcacgaa
     2761 gcgggtcgaa gggttgatcg agtcgcgcgt caacccggcg ctcgaaagtc tggcagctta
     2821 cgtatcgggc gattacgcgg cgagggctcc ccgatcggtg gggctcggac agtacgccgg
     2881 gggcaaggcg tactaccggc ggctggtcaa gcagtacacg accctcgacg tcacgccgga
     2941 ggaggtgcac cggttcggcc tcgctgagat cgaaaggctg aaccgggagc tcgacgaagt
     3001 gcgggtcgcg gtcggtttcg acgggacgct tgccgagttt cgccggcagc tgaagagcga
     3061 tccgagattc gttcctgcga cttccgacgc gatcggcgag cggttgctcg aggcacagaa
     3121 gcgcatcggg tccaagattc cgctcttctt cgggaagatg ccttcggcgc ccgccggcat
     3181 ccggcggctc gcgtcgacgc tcgaaggcgc gatgacctac ggctactacc aggccccgac
     3241 cgtctcggac gcccgcggct actactacta caacggctcg aagctcggcg agcgctcgat
     3301 gttgaacgcc gcggcgctca tctacgacga gctcgtgccg gggcatcact ttcaggtctc
     3361 cctgcagcag gagaactccg aactccccag cttccgccgg gaggcccgct gggccgccta
     3421 cacggagggc tggggggaat acgcctcgtc gctcgccgcc gaaatgggga tgtacgacga
     3481 cccttacgac cgcgccggcc gtctcatgag ggatgcgtat ctctccgcgg ggctcgtcgt
     3541 cgacaccggg atgaacgcgc tcggctggag ccgcgcgcga gcgacggcat acctgaagga
     3601 gaacacgctc gaatccgaca cgcagatcgc ggcggagatg ctgcgatact cgtgcgacgt
     3661 cccggggcag gctctcgctt acaagatcgg cgcggcgaag attcgcgagc tgcgtgagaa
     3721 agcgtcccgc gaactcggga ggtcgttcga cgtgcgccgt ttccacgacg cggtgctcgg
     3781 atcgggcgcc atgccgctcg cggtcctcga gcggcacatc gactggttca tcgaaagcca
     3841 acgcacccgc ctcgcgagcc gctgagcgaa aagccgcggg cccgaatgcc cgccgcttaa
     3901 ccgccaaccc tggaaactcc agtgtcgact ttgaaacacg gtgtggggga gccgccgact
     3961 cgtgaacgga ccactgtccc aacctgaagg acgagtggcc gccagatttt cttacctcgt
     4021 cttcgcgaag cgcagagtct cgcccgagtc gacgttgcgc acccggactc ctcccggctc
     4081 gacttccacc gtccatttgc cgaccgcgcc ggggagcgcc cgcgcgcacg tggtcgctcc
     4141 tgcgagctcg ccgacgtagc cgtcgccgct cggatggaag tagaggttgc aggccgcttc
     4201 cagctcctcc tgcgtaaacc gcgtcgccgc gcgggagagc ggagtcaccg tcgagtcgaa
     4261 gtggggaacg tagaccgcgt ggacgtcccg cccctgcgtt tcgagccgca gcaggccgac
     4321 gcgccggacg ttcgtgtcgc gataccggcc ggaggacgtc aggaacagat cgaaccgcgt
     4381 gcccgcgctc gcgccgacgg tcgaaaggtc gaggcgcagg tcgttgccgg gcgtcgtccc
     4441 gcgaaagcgt cccgagagca tcgaggcgag atcggtcagg ctcgaacgcg cgctccctcc
     4501 cgtgcgcgga gccgccgacg ataagcaggc gagcagcgtc aggaggagcg cggcgccgcc
     4561 gagagcgccg atccttaaag tcgtcttcat gaatcgagta cctccatccg gagagatgcc
     4621 agatccgttc cagcggtcca aggcttgcag tcgtccgtct cgttgtgaac gtacggaaga
     4681 gcccgcctcg gggtccgcgc cgatttctca ccgggcttcc ggcagcgacg gcgctctcgc
     4741 tggtgctcgg gaccgccgga tgctcgtcgg ttttcgaaag acccgagctc cagttccgtg
     4801 gaatccacgt caactcgatc gggttcgacg gcgcgtcgct cgagatcctg gtcgacgtct
     4861 acaaccccaa ctcctaccgg ctcgggctcg agcgcctctc ctacgacctc gtgatcgaga
     4921 acgtgcactg ggggctgggg tccctgacgg cgccgatcgg ggtggagggc cacgatgcca
     4981 cgatggtccg cctgccgctc gacgtctcct ggtcgcggct cggcgacgtc ggtcgggagg
     5041 cgctccggac gggaaccgtg aattacggca tctccgggga gatgacggtc gcgacctcgc
     5101 tcggaaacat cgacgtgccg tactcgaagg cggggcgctt ctcggtcctc accggccagg
     5161 agagctccga ccccggcccg attccgcccg atccgacgtg cggcttccgg caggcgattt
     5221 actcgatcga gaccacgact ccgttctcga agaccacttt ctttccgacg ctcctgtaag
     5281 tccacgtctc gcg
//
LOCUS       DASWAV010000191         1955 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U2665
            k141_171212, whole genome shotgun sequence.
ACCESSION   DASWAV010000191 DASWAV010000000
VERSION     DASWAV010000191.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960316
            Sequence Read Archive: SRR10260007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 1955)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1955)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:47:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,929
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1955
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_171212"
                     /isolate="SMAG_U2665"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.36444 N 132.31889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(172..756)
                     /locus_tag="VF958_03845"
     CDS             complement(172..756)
                     /locus_tag="VF958_03845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9148273.1"
                     /translation="MSTIVLILAIAFALSWIPAVIVFLIARRRNAGDRVIVCPETHAT
                     EVVRLDAGRAAMTDLRGDKELRLESCSRWPERADCGQDCLAQIEAAPDGCLVRERLEN
                     WYRGAECALCGMAIGPIHWFAYRPGLLTPDRRALSWEEIPAKALPPALENDQPICSDC
                     LLAESFREKFPDRVLDDPWHKVDRRETRAPGPMA"
     gene            complement(924..1280)
                     /locus_tag="VF958_03850"
     CDS             complement(924..1280)
                     /locus_tag="VF958_03850"
                     /inference="COORDINATES: protein motif:HMM:NF012422.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HEX9148274.1"
                     /translation="MKKTFLRLGDGGSPASDQRRKRTSGNGAKPRRLSPKERQLTTRE
                     REILRFLAFGMGTAAIAKELSISPATVRNHAQRILAKLQVHSRLAAVARGYAIGLLPS
                     PQWRPAAMPLAEPPPA"
     gene            complement(1252..1749)
                     /locus_tag="VF958_03855"
     CDS             complement(1252..1749)
                     /locus_tag="VF958_03855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9148275.1"
                     /translation="MLSRRPGLVPVASFLLLFGVPLAVSFALDRAASSGTPAPVKFFD
                     ARSQAAEFIGYSRGIELTPAQVRIRETALRAMAAPCCKNFPLATCCCPCNLAKSSWGL
                     ANYLIARKGATADEVKVAVGGWLAFVNPNGFSGDACDTANGCARRFSHNGCGGMDEEN
                     LLAAR"
BASE COUNT          336 a          653 c          667 g          299 t
ORIGIN      
        1 accgccggtc gaactccgct tcgctccagc ggcggaagcc gcggcagaag atcccgcgct
       61 cggccgcgat ctggcagagt gagtgctgtt cgtccgtcat ggcgaggagc gacgcgcgca
      121 aacgcgagac cgcctcctcc cggtgccaga cgtgcggaga cgctccgcgg gtcacgccat
      181 cgggcctgga gcgcgggttt cgcgacggtc gaccttgtgc cacggatcgt ccaggacgcg
      241 gtcggggaac ttctcccgaa aggactcggc gagcaggcaa tcggaacaga tcggctgatc
      301 gttctcgagg gccggcggca acgctttggc cggaatctct tcccacgaaa gcgcgcggcg
      361 atcgggcgtg agcaggccgg gacgatatgc gaaccagtgg atcggtccga tcgccatccc
      421 gcaaagcgcg cactccgcgc cgcgatacca attctcgagc cgctcgcgaa cgaggcagcc
      481 gtctggagca gcctcgatct gcgcgagaca gtcctggccg cagtccgctc tttcgggcca
      541 gcgcgagcaa gactcgagac gcagctcctt gtctccgcga aggtcggtca tggcggcgcg
      601 gccggcgtcc agccgaacga cttccgtggc gtgcgtctcg ggacacacga tcacgcgatc
      661 acccgcgttc cgacgccgcg cgatgagaaa aacgatcaca gccgggatcc aggaaagagc
      721 gaacgcgatg gcgagaatga ggacgatcgt gctcatggcc tacctcctcg acgttcgact
      781 gtagccggag ccctttccgg cggcatggtg caaatgaatc agccgcgccg ggctgccgcc
      841 gacggcgatg ggggggcccc gttccctgtc accggttcag tcagccggcg accggttcgg
      901 cctggcgcca ggattcgctg gcttcaagcc ggcggaggtt ccgcgagagg catcgcggcg
      961 ggccgccact gcggcgaagg aagcaggccg atcgcgtaac cgcgagcgac tgccgccagc
     1021 cggctatgga cctggagctt cgcgaggatt cgctgggcgt gatttcgaac ggtcgccggc
     1081 gagatcgaga gctccttcgc gatcgctgcg gtccccatgc cgaacgcgag gaagcggaga
     1141 atttctcgct cgcgagtcgt gagctgtcgc tccttcggcg acagccgacg cggcttcgcg
     1201 ccattcccgg aggtccgttt gcgccgctgg tcggaagcgg gagagccgcc gtcaccgagc
     1261 cgcaagaagg ttttcttcat ccattcctcc gcagccgttg tgggagaagc gccgggcaca
     1321 gccgttcgcc gtgtcgcacg catctccgct gaagccgttc gggttgacga aggcgagcca
     1381 tcctccgacg gctaccttca cctcgtccgc tgtcgcgccc ttgcgggcga tcaggtagtt
     1441 cgcgagtccc caggagctct tcgcgaggtt gcatgggcag cagcaggtcg cgagcgggaa
     1501 gttcttgcag cagggggcgg ccatcgcgcg gagcgccgtc tcacggatcc tgacctgcgc
     1561 cggcgtgagc tcgatccccc tcgagtagcc gatgaactcc gcggcctggg agcgcgcgtc
     1621 gaagaacttc accggagccg gcgtcccgga ggacgcggcg cggtcgagcg cgaacgacac
     1681 ggcgagcgga accccgaaca ggagcaggaa cgaggcgacc ggaacgagac cgggacgacg
     1741 agagagcact ggttctcctc ccgcgggagc gtcttcctcc cgctcgatcg caggctaatc
     1801 tccggcgccc gcgtccactt gatgcaaacg aatcagcacc ctttttggca aaaaccggga
     1861 gagaatcgac ccgtgagcga cgcgctggac ctgacagccg agctcctgtc cgcgcgcctc
     1921 cgacgcgccg tggatctcct gtggcgcgag accgt
//
LOCUS       DASWAV010000192         6144 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U2665
            k141_730132, whole genome shotgun sequence.
ACCESSION   DASWAV010000192 DASWAV010000000
VERSION     DASWAV010000192.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960316
            Sequence Read Archive: SRR10260007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 6144)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6144)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:47:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,929
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6144
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_730132"
                     /isolate="SMAG_U2665"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.36444 N 132.31889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..324)
                     /locus_tag="VF958_03860"
     CDS             complement(<1..324)
                     /locus_tag="VF958_03860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9148276.1"
                     /translation="MSSIPIRRLFAATSLLFLAVLAISPAKSALRSYRSVQRHYRQLG
                     MARARSLKAARDYEQRPVAIQQVWLRDFENRVDRCTTCHLGVNDPLMKGASEPLRLHV
                     QTAHTP"
     gene            complement(335..1045)
                     /locus_tag="VF958_03865"
     CDS             complement(335..1045)
                     /locus_tag="VF958_03865"
                     /inference="COORDINATES: protein motif:HMM:NF012262.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9148277.1"
                     /translation="MSALPPIHIVESADPLATPRRVALVRPDIRPAVRRREERWVMTF
                     PHLIVREVILFQVVVIGLALGAIFFDAPLQGIANPLETPNPAKAPWYFLGLQELLHYF
                     PPVVAGVLMPTLVVIALVVIPYARVNLTAGPLWASRPGRTAAVVGAVTVFLIALFATF
                     ACWPIVVPTALIAAAMFAARGGESGGWLRRRLARVTLPEWIMTWFVVIATILTVIGTF
                     FRGPGWSLVWPWNAAVFG"
     gene            complement(1042..1806)
                     /locus_tag="VF958_03870"
     CDS             complement(1042..1806)
                     /locus_tag="VF958_03870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011197261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b N-terminal domain-containing
                     protein"
                     /protein_id="HEX9148278.1"
                     /translation="MRELSLGSLGSRVRDTALYRSIVRHGIADTNRNRSLVIFENLFL
                     HVHPVKVREKTLRFSHTYWLGGITLACALVLIATGILLMFYYHPSVPQAYLDMKELAF
                     VVENGTFLRNLHRWAAHLMVFAAFLHMIRVFYHHAYRPPRQFNWVVGVVLLLVTLLLS
                     YTGYLLPWDQLAFWGVSVGTNMAKEAPLIGEQIRFLLLGGHVVSENALLRFYVLHCVV
                     MPVALLLLLSVHIWRVRKDGGVQGPPEESDERGEIP"
     gene            complement(1803..2294)
                     /locus_tag="VF958_03875"
     CDS             complement(1803..2294)
                     /locus_tag="VF958_03875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012645226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske 2Fe-2S domain-containing protein"
                     /protein_id="HEX9148279.1"
                     /translation="MPEDPEDLTGVEEDEPSRRDLFSRVGLAACAVAAAGSGIVTLDF
                     LKPKVLFEPPTRFRAGNPLDYPEGVVRFNKQQRAYVIGGPGGVYALSAVCTHLGCITR
                     FLSDEKVIACPCHGSRFDLEGNVIHGPAPRPLPWLEMQVDATGTLLVDTSVTIPHGRI
                     FKA"
     gene            complement(2287..4116)
                     /locus_tag="VF958_03880"
     CDS             complement(2287..4116)
                     /locus_tag="VF958_03880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012632832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEX9148280.1"
                     /translation="MGGFNAEWLERNFPCKQACPVHTEAGKYVTLIAQGRYREAYAVA
                     RRPNPLASICGRICAAPCETVCRRGELDAPIAIRALKRFVTERFGVESMMDFSVLTEI
                     YGERRGRYSERIAVIGSGPAGLACAHDLALVGYPVTIFEATSVAGGMLRLGVPEYRLP
                     RALIQLEVNAILSLGVELRTNARLGRDFTLADLRKQGFTAFFLGVGAMRSRDLTIPGV
                     ELDGVLRGIDYLLNINLGYKVEMGQHIVVIGGGNVALDVARTAARGGEQANLQRNLSI
                     VQALDVARSAIRFGARAVTVCSLESESEMPAAKEEMEEAAKEGIRFLHRVGPKRLVGE
                     KGAVTGIEFLKVSRVFDETGRFSPQFIEGSETILPTDTVIIAIGQTGDFSFLRPEDGI
                     ETRGGRIVIDPDTLATTAPGVWAGGDAAFGPRIAINAIADGKKGAKSIDEHFRGSLRP
                     ERDVAVEIDLHSRYERDLDFEGIPRQKPPTRPISRRIGIAEVEECLGEREAHLEGKRC
                     LHCWTNTIFEEDPRAGTECILCGGCQDICPEDCIEILPVALVSEPPPEVWELAPILEN
                     GKRVGSIFIKDEETCIRCGLCARRCPVGTITMQSFHTKEDAVA"
     gene            complement(4123..4707)
                     /locus_tag="VF958_03885"
     CDS             complement(4123..4707)
                     /locus_tag="VF958_03885"
                     /inference="COORDINATES: protein motif:HMM:NF012355.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HEX9148281.1"
                     /translation="MEMKKIFLATLSLGLALPVTAGDLHGKVTCKSVRDSADAVVYVA
                     AIPGKTFPAPKEHAKMDQANLVFHPHVLPVQVGTTVDFLNSDAQLHNVFTPDACADKF
                     NLGTWPKGQIKSYEFKKECAATLLCKVHPEMEGFVVAVATPYFAVTKADGSYRIPDVP
                     DGAYTVKVWHPKLKGTQKAVTVAGATAADFEISK"
     gene            complement(4714..6114)
                     /locus_tag="VF958_03890"
     CDS             complement(4714..6114)
                     /locus_tag="VF958_03890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9148282.1"
                     /translation="MNTIIRRLQRALFCLVAGLILSDARPAAAIPAFSRKYQTSCQTC
                     HVIFPKLNAHGEAFRLNGYRMPAEMEEEVKQAPVSLGSEAYERIWPEMVYPSDIPGSI
                     PLALNTKFANVYESSFVDEGRTIIHNDFQFPQEVNLFAGGTLGKTFSFFGELTWAERP
                     DGGSDVEIEHARIHVNSPFGPEHLVNFKIGKFAPDLEDGFQEMWLMTDNGIESLFAFN
                     PIGFRGGSGLAEEDAPAGISLPDLVKGIEMYGVAGHRLFYTLGVTNGIGGAGDTFDGN
                     SSKDVYARVDYKIGGMGLDGDTTGVTLPPENWRETSLRIGALGYLGNGSGIDFDVTDD
                     AGAPFKMQDRHSQRVGLFASWYFGDLNLFGVALHGRDKLRLFDAETSATLSDTTRTYD
                     VWFAQADYVIRPPFQVSLRYENLRPADPEARTLKTVNANFSFLIRANIKAMLEYRRDL
                     RESKNYSLNTLLRFAY"
BASE COUNT         1122 a         1945 c         2023 g         1054 t
ORIGIN      
        1 cggcgtgtgg gcggtttgca cgtggaggcg aagcggctcg gaagcccctt tcatcaaggg
       61 atcgttcacg cccaggtgac acgtcgtgca gcggtccacc cggttctcga agtcgcgcag
      121 ccacacctgt tgaatcgcca cgggtcgctg ctcgtagtcc cgcgccgcct tgagactcct
      181 ggctcgggcc attccgagct ggcggtaatg gcgctgcacc gatcgataag accggagtgc
      241 actcttcgcg ggtgagatcg ccagcaccgc caggaaaagg aggctcgtcg cggcgaagag
      301 ccggcgaatc ggaatggagc tcaccgtccc tcccctaacc gaatacggcc gcattccacg
      361 gccagacgag ggaccaaccg gggccgcgga agaaggtccc gatgacggtc aggatcgtgg
      421 cgatcaccac gaaccaggtc atgatccatt ccggcagcgt gacccgcgcg aggcgccgtc
      481 gcagccatcc cccactctcc cctccgcgcg cggcgaacat cgctgccgcg atcagggccg
      541 tgggaacgac gatcggccag catgcaaagg tggcgaagag ggcgatcagg aaaacggtaa
      601 cggccccgac tacggccgcg gttcgtcccg gacgggaggc ccagaggggg ccggccgtca
      661 agttgacccg cgcatacggg atcacgacga gcgcgatgac gacgagcgtg ggcatgagga
      721 ccccggcgac caccggaggg aagtagtgca gcaattcctg cagccccagg aagtaccacg
      781 gagccttggc gggattgggg gtctccaggg ggttcgcgat gccctgaagg ggggcatcga
      841 agaagatggc gcccagcgcg agcccgatca caacgacctg gaaaaggatc acctcgcgca
      901 cgatcaggtg ggggaacgtc atcacccagc gttcctcgcg gcggcgaacg gccgggcgga
      961 tgtcggggcg caccagcgcg acccggcgtg gcgtggcgag aggatcggcg gactcgacga
     1021 tgtgaatcgg cggcagagca ctcatggaat ttctccgcgc tcgtccgact cctcgggcgg
     1081 cccctggact cctccgtcct tgcgaactcg ccagatgtga acggagagga gaagcaggag
     1141 cgcgacgggc atgacgacgc agtgcaggac gtagaagcgc agaagggcgt tctccgagac
     1201 gacgtgtcca cccagcagaa ggaaacggat ctgctccccg atcagcggcg cttctttggc
     1261 catgttggtc ccgaccgaga cgccccagaa cgcgagctgg tcccagggga gcagataccc
     1321 ggtataagaa agaagcagcg tcacgaggag gagaacgacg cccacgaccc agttgaattg
     1381 ccggggcggg cgataagcgt ggtggtagaa gacccgtatc atgtggagaa aagctgcaaa
     1441 gaccatcagg tgcgcggccc agcgatgaag gttgcgcagg aacgtgccgt tctcgacgac
     1501 gaacgcgagc tccttcatgt cgaggtaggc ctgcggcacg gaagggtggt agtagaacat
     1561 cagaaggatt ccggtcgcga tgagcacgag cgcgcatgcc agcgtgatcc cgcccagcca
     1621 gtaggtgtga gaaaagcgca gcgtcttctc ccggactttg acggggtgca cgtgcaggaa
     1681 gagattctcg aagatgacga gcgaacggtt gcggttcgtg tcggcgatgc catgtcgaac
     1741 gatcgagcgg tacagagccg tatcccgcac gcggcttccg agcgagccga gcgacagctc
     1801 tctcatgcct tgaagatcct gccgtggggg atcgtcacgc tcgtgtcgac gaggagggtg
     1861 ccggtcgcgt ccacctgcat ctcgagccat ggcagaggac gcggggccgg tccgtgaatg
     1921 acgttcccct cgaggtcgaa tcggcttccg tggcacgggc aggcgatgac cttctcgtcg
     1981 gacaggaatc gggtgatgca tcccaggtgc gtgcacacgg ccgacagggc atagacgccc
     2041 ccgggcccgc cgatgacgta cgccctctgc tgcttgttga accgcaccac accttcgggg
     2101 tagtcgagcg gattgcccgc gcgaaagcgg gtcggcggct cgaagaggac cttgggcttc
     2161 aggaagtcca gggtcacaat cccggatccg gccgcggcca cggcgcaggc ggccaggccg
     2221 acgcggctga agagatcgcg ccggctcggt tcgtcctcct cgacaccggt cagatcctcg
     2281 ggatcttcag gcaacagcgt cctccttcgt gtggaagctc tgcatggtga tcgtgcccac
     2341 cggacagcgc cgggcgcaga gcccgcagcg gatgcaggtc tcctcgtcct tgatgaagat
     2401 cgatccgacg cgttttccgt tttcgagaat cggagcaagc tcccacacct cggggggcgg
     2461 ctcgctcacc agggcgaccg gcaggatctc gatgcagtcc tccgggcaga tgtcctggca
     2521 acctccgcac agaatgcact cggttccggc ccgcgggtcc tcctcgaaga tcgtgttggt
     2581 ccagcagtgc aggcaccgtt ttccttccag gtgcgcttcc cgctcgccga ggcattcctc
     2641 gacctcggcg atcccgatgc gccgggagat cggccgggtc gggggcttct gccgggggat
     2701 gccctcgaag tccaggtccc gctcgtagcg ggagtggaga tcgatctcga cggcgacgtc
     2761 ccgctcgggg cgcagcgagc cgcggaaatg ctcgtcgatc gactttgccc ccttcttccc
     2821 gtcggcgatc gcgttgatcg cgattcgcgg cccgaacgcc gcgtcaccgc cggcccagac
     2881 ccccggagcg gtcgtggcaa gcgtgtcggg atcgatcaca atgcgccccc cgcgcgtctc
     2941 gattccgtct tcggggcgca ggaacgagaa gtctcccgtc tggccgatgg cgatgatcac
     3001 ggtgtcggtc gggagaatcg tctcggagcc ctcgatgaac tgcggagaga agcggcccgt
     3061 ctcgtcgaag acccgggaaa ccttgaggaa ctcgatcccg gtcaccgctc ccttctcgcc
     3121 gaccagccgc ttggggccca cgcggtggag gaagcggatg ccttccttcg cggcctcctc
     3181 catctcctcc tttgcggcgg gcatctcgct ttccgattcg agcgagcaga ccgtcaccgc
     3241 gcgggcgccg aaccggatgg cgctgcgcgc cacgtcgagc gcctgcacga tggaaagatt
     3301 gcgctggaga ttcgcctgct cgccgccgcg ggccgccgtc cgtgcgacgt ccagagccac
     3361 gttgccgccc ccgatgacga cgatgtgctg gcccatctcg accttgtaac cgaggttgat
     3421 attgagcaga tagtcgatgc cgcggagcac gccatcgagc tcgacgcccg gaatggtcag
     3481 gtcgcggctg cgcatcgcgc cgacaccgag aaagaacgcc gtgaatccct gtttcctcag
     3541 gtccgccagc gtgaagtcgc gcccgagccg ggcattggtt cggagctcga cccccaggga
     3601 gagaatggca ttgacctcga gctggatcag ggctcgcggg aggcggtact ccgggactcc
     3661 gaggcggagc attccccccg cgacagacgt tgcctcgaaa atcgtcacgg gatagcccac
     3721 gagggccagg tcgtgcgcgc aggcgaggcc ggcgggtccg gacccgataa cggcgattcg
     3781 ctcggaatag cggccacgcc gctccccgta gatctctgtc aggacggaga agtccatcat
     3841 gctctcgacg ccgaacctct ccgtcacgaa ccgcttcaat gcccggatcg cgatcggagc
     3901 gtcgagttct ccccggcggc aaacggtttc acacggcgcg gcacagatgc gcccgcagat
     3961 cgaggcgagc ggattggggc gccgggccac ggcgtaggcc tcgcggtacc ggccctgggc
     4021 gatgagcgtg acgtacttgc ccgcctccgt gtggacgggg cacgcctgct tgcaggggaa
     4081 gttacgctcg agccactcgg cgttgaaccc gcccaaggtc gcctactttg agatctcgaa
     4141 gtctgcggcg gtcgctcccg ccacggtcac cgctttctgc gtccccttca gcttcgggtg
     4201 ccagaccttg acggtgtagg ccccatcggg cacgtcggga atccggtagc tgccgtcggc
     4261 cttcgtcacc gcgaaatacg gagtcgccac ggcaacgacg aagccttcca tctccgggtg
     4321 aaccttgcag aggagcgtcg cggcgcactc cttcttgaac tcatagctct tgatctgccc
     4381 cttgggccag gtccccaggt tgaacttgtc ggcacaggcg tcgggagtga acacgttgtg
     4441 gagctgggcg tccgagttca agaaatcgac ggtcgtgccg acctgcacgg gcagcacatg
     4501 gggatggaag acgaggttcg cctggtccat cttggcgtgc tccttgggcg ccgggaatgt
     4561 cttgccggga atcgccgcca cgtacacaac ggcgtcggcg ctgtcccgca cgctcttgca
     4621 ggtgaccttg ccgtgaaggt cgcccgccgt gaccggcagc gccagaccga gagagagagt
     4681 cgccaggaag atcttcttca tttccatcgc tccttagtag gcaaagcgca atagggtgtt
     4741 gagagagtag ttcttcgatt ccctcaggtc tcggcggtac tcgagcatcg ccttgatgtt
     4801 cgcccgaatg aggaagctga agttggcgtt caccgtcttg agcgtccggg cttccggatc
     4861 cgcgggtcgg agattctcgt atcggaggga gacctggaac ggcggccgaa tcacgtagtc
     4921 cgcctgcgca aaccagacgt cgtacgtacg cgttgtgtcg ctcaaagtgg cgctcgtttc
     4981 tgcatcgaac agccgcagct tgtcgcggcc gtgcagagcg acgccgaaga gattgagatc
     5041 gccgaagtac catgacgcga agaggccgac tcgctgggaa tggcgatcct gcatcttgaa
     5101 aggtgcacct gcatcgtcgg tcacgtcgaa gtcgattccg gagccattgc ccagataccc
     5161 gagagcgccg attctcagcg acgtctcgcg ccagttctct ggcggcaacg tcacgccggt
     5221 cgtgtcgcca tcgagaccca tgccgccgat cttgtagtcg acgcgtgcgt agacgtcctt
     5281 cgaggagttg ccgtcgaacg tgtcgcccgc gcccccgatg ccattcgtga caccgagcgt
     5341 gtagaagagc cggtgtcctg ccactccgta catttcgatc cccttcacga ggtccggcag
     5401 gctgatgccg gcgggcgcgt cctcttcggc gagaccggat cctcctcgga acccgatcgg
     5461 attgaaggcg aagagcgact cgatgccgtt gtcggtcatc agccacattt cctgaaagcc
     5521 gtcctcgaga tcgggagcga actttccgat tttgaagttg acgaggtgct ccggcccgaa
     5581 gggagagttg acgtggatcc gcgcatgctc gatctcgacg tcgctccccc cgtcggggcg
     5641 ctccgcccag gtcagctccc cgaagaagct gaaggtcttt ccgagcgttc cgccggcgaa
     5701 gaggttgacc tcctgcggga actggaagtc gttgtgaatg atcgtccgcc cctcatccac
     5761 aaacgacgac tcgtagacgt tggcgaactt cgtgttcagg gcgagcggga ttgagcccgg
     5821 aatatcgctc ggatagacca tctccggcca gattcgctca taggcctcgg agccgagcga
     5881 aaccggtgcc tgcttgacct cctcttccat ctcggccggc atccggtacc cgttcagccg
     5941 gaaggcttcg ccatgcgcgt tcagtttcgg aaaaatcacg tgacacgtct ggcaggacgt
     6001 ttggtatttg cgcgagaacg cgggaatggc ggcggccggc ctcgcgtccg agagaatcag
     6061 tccagccacc aggcaaaaga gggcccgttg gaggcggcga atgattgtgt tcacggagga
     6121 ctctcctttc atcaccctgg gtca
//
LOCUS       DASWAV010000193         3540 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U2665
            k141_690444, whole genome shotgun sequence.
ACCESSION   DASWAV010000193 DASWAV010000000
VERSION     DASWAV010000193.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960316
            Sequence Read Archive: SRR10260007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 3540)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3540)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:47:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,929
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3540
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_690444"
                     /isolate="SMAG_U2665"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.36444 N 132.31889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..420
                     /locus_tag="VF958_03895"
     CDS             <1..420
                     /locus_tag="VF958_03895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008937404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HEX9148283.1"
                     /translation="VESLAARERLLLFCGRYEGLDERARLLGLFDAEISLGDFVLPGG
                     ESAALALTEAVSRLVPGVVGSAESLEEESFARERLDYPHYTRPREFRGLVVPETLLSG
                     DHAKIRRWRRARALERTRERRPDLLERRPEEPGEGEE"
     gene            540..890
                     /gene="rplS"
                     /locus_tag="VF958_03900"
     CDS             540..890
                     /gene="rplS"
                     /locus_tag="VF958_03900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014123709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HEX9148284.1"
                     /translation="MSDILLDVEKPSLRRDVPNFAPGDSVRVHVKVREGEKERIQVFA
                     GIVIARRSGGARETFTVRKISSGIGVERIFPLHSPVIDKIEVERKGAVRRAKLYYLRE
                     RRGKAARIGEKRTE"
     gene            905..1621
                     /locus_tag="VF958_03905"
     CDS             905..1621
                     /locus_tag="VF958_03905"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020381614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HEX9148285.1"
                     /translation="MRTDPLSALFAELGRLSELDRIEKELQRRGYVLVAGVDEAGRGA
                     LAGPVVAAAVILPARCVLVGLDDSKQVDPACRERLAAAIRRHAVAVGVGVVSAAEIDV
                     RDILRASLAAMRVAVLALDPQPEAVLVDAVFIPGVRVPQLPIIHGDALCSSVAAASIV
                     AKVERDRILDELGRRFPVYGFEHHKGYGTPEHWQSLSAYGPCSEHRLSYHGVVPEIGE
                     PEPLAPPLTRGRAGKPGRSG"
     gene            1631..>3540
                     /locus_tag="VF958_03910"
     CDS             1631..>3540
                     /locus_tag="VF958_03910"
                     /inference="COORDINATES: protein motif:HMM:NF025910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX9148286.1"
                     /translation="MSGARDRIVQSAEKNLSRGRLDQALADYLSLLKESPGDTFALNK
                     AGDLCVRMGRPADAIEHFNRIAEVYSADGFFLKAIAIYKKINKIDPTRLDVYDRLADL
                     YAKQGLTQDARSHYAVLAEQWLKRNDLKGAISAYSKLAALDPADLKVQARLADLHRVA
                     GETDQAVARYDAIGGLLLRRGAYGEAITFFRKALELDSGDSGARMQMVRAYLAIDDLN
                     SAVAALEPAPRNAEACVLLGEALLARGDRAAAASAAQDAINLEPENEIARAFLLKIRL
                     AEGTPDRALEAIAPAVDAAVGAGDFARAAAHLRPILDAYPDHRATLERTAEIREAGGE
                     AEESVAVRLTLAQAAEKRGDLPAAVEHYRRILSAVPGHAEARRRLDELVPPAAAPVAE
                     VPAAPRAVRNEVQAALEEADVLARYGLKDRAVERLRAVAKLHPEATAARERLVELLVE
                     LANPAAAREADALAEDYRKQGRESLAVALYARVGLPVPVGRPAPPGAPATGSVPQGSG
                     PEVVFDEFEIELPGAGASPAVGAPAFDPFAELPEASASAPPLAAAAPAGASSPGAARL
                     TPHAPGDTGFGLLAPELGSLIEEKMQRGAAEAPRRDLHSPRPKVVDDENLFADERQFF
                     NLAEELEKELSE"
BASE COUNT          542 a         1225 c         1231 g          542 t
ORIGIN      
        1 gtcgaatcgc tcgccgcccg cgagcggctg ctcctcttct gcggccggta cgaagggttg
       61 gacgagcggg ctcgacttct cggcctcttc gatgccgaga tttcgctcgg agatttcgtg
      121 ctgcccggcg gagagtccgc cgcgctcgcg ctgacggagg ccgtatcccg tctcgtgccg
      181 ggcgtcgtgg gatccgcgga atctctcgag gaggagtcct tcgcgcgcga gcgcctcgac
      241 tacccgcatt acacgcggcc gcgcgagttt cgcggcctgg tcgtcccgga gacgctcctc
      301 tccggcgacc atgcgaagat ccgccgctgg cgccgcgccc gcgcgctcga acggacccgt
      361 gaacgcagac cggacctcct cgagcgccgg ccggaggagc cgggcgaggg cgaggaatga
      421 gtctcgagtc ccgaccgacc cgactttggg acttgagacc cgggactgta taatccgccg
      481 tcccgctcgg ccgagcggga gcatccctcg ggtcttccgg agtacgagag aggtttcata
      541 tgagcgacat cctgttggac gtggaaaagc cctcccttcg cagggacgtt ccgaatttcg
      601 cgccgggcga ctcggtgcgg gtgcacgtga aagtccgcga aggtgaaaaa gaacgcatcc
      661 aggtttttgc gggcatagtg atcgcccggc gaagcggggg ggcgcgggaa actttcacgg
      721 tgcgcaagat ctcgagcggg atcggcgtcg aaaggatctt tccccttcac tcgccggtga
      781 tcgacaagat cgaggtcgaa cgaaagggtg cggttcgccg ggcgaagctc tactacctga
      841 gagagcgtcg gggcaaggcc gcacgcatcg gggaaaagag aaccgaatag gcggaatacc
      901 gcccgtgagg accgatcctc tctctgccct gtttgccgag ctcggacgcc tctccgagct
      961 cgaccgcatc gagaaggagc tgcagcgccg gggttacgtt ctcgtcgccg gagtggatga
     1021 agccgggcgg ggcgccctcg ccggtccggt ggtcgcggcc gcggtgatcc tgccggcccg
     1081 atgtgttctc gtcgggctcg acgactccaa acaggtcgat cccgcgtgcc gggagcgtct
     1141 cgccgcggcg atccgccgac atgcggtcgc cgtcggcgtg ggcgtcgtca gcgcggcgga
     1201 gatcgacgtc cgcgacatcc ttcgcgcgag tctcgcggca atgcgggtag cggttctcgc
     1261 gctcgatcct cagcccgagg cggtgctcgt cgacgcggtc ttcatccccg gcgttcgtgt
     1321 tccgcagttg ccgatcattc acggcgacgc gctctgctcg tcggtcgccg ccgcatcgat
     1381 cgtcgcgaag gtcgagcgcg atcggatact cgatgagctc ggccggcggt ttcccgtgta
     1441 cggattcgag caccacaagg gctacgggac cccggagcac tggcagtcgc tctccgcgta
     1501 cggaccgtgc tccgagcacc gcctgagcta ccacggagtc gtgccggaaa tcggcgagcc
     1561 ggaacctctc gcccctcctt tgacgcgcgg tcgggccggg aaacccggcc gtagcggtta
     1621 aaatcttcca atgtccggtg cgcgcgaccg gatcgttcaa tccgccgaga agaacctgtc
     1681 gcggggaagg ctcgatcagg cgctcgccga ctatctgagt ctcctgaagg aaagccccgg
     1741 cgacaccttc gctctgaaca aggcggggga cctttgcgtc cggatgggtc ggccggcgga
     1801 cgcgatcgag cacttcaacc ggatcgcgga ggtttactcg gccgacgggt tcttcctcaa
     1861 ggcgatcgcc atctacaaga aaatcaacaa gatcgatccg acccgcctcg acgtgtacga
     1921 ccgcctggcc gatctctacg cgaagcaggg cctgacccag gacgcccgga gccactacgc
     1981 cgttctcgcc gagcagtggc tgaagaggaa cgacctgaag ggcgcgatct cggcgtattc
     2041 gaagctggcg gcgctcgacc ccgccgactt gaaggtccag gcgcgactcg ccgacctcca
     2101 tcgcgtcgcc ggggagaccg atcaggccgt cgcgcgctac gacgccattg gcgggctgct
     2161 cctccggcgc ggggcgtacg gcgaggcgat cacgttcttc cgcaaggcgc tcgagctcga
     2221 ttccggcgac tccggcgccc ggatgcagat ggtccgggcg tacctggcga tcgacgacct
     2281 caactcggcg gtcgccgccc tcgaacccgc cccccgaaac gcggaggcgt gcgtgctcct
     2341 cggggaggcg ttgctcgccc gaggcgatcg agctgccgcg gcttccgccg cgcaggatgc
     2401 gatcaatctc gagccggaga acgagatcgc ccgcgcgttc ctcctgaaga tccgcctggc
     2461 cgaaggaacc ccggaccggg cgctcgaggc gatcgcgccc gcggtcgacg cggctgtcgg
     2521 agcgggcgat ttcgcccgcg cggcggcgca ccttcgcccg atcctcgacg cgtacccgga
     2581 ccatcgcgcg acgctcgagc gaaccgcgga aatccgggag gcgggcggcg aggcagagga
     2641 gtccgtcgcc gtccggctca ctctcgcgca agcggcggaa aagcgcggcg acctgccggc
     2701 ggcggtggag cactaccgtc gaatcctctc cgcggtcccc ggccacgcgg aagcgcggag
     2761 gcgcctggac gagctcgtcc cgccggccgc ggctccggtc gccgaagtgc cggccgcgcc
     2821 gcgcgccgtc cggaacgaag tccaggccgc gctcgaagag gccgatgtcc tcgctcgcta
     2881 cgggctcaag gaccgcgccg tggaacggct gcgcgccgtc gcgaaactgc accccgaggc
     2941 gaccgctgcg cgtgagcggc tggtcgagct cctggtggag ctcgcgaatc ccgccgcggc
     3001 gcgggaggcg gacgccctgg cggaggacta ccgcaagcag gggcgcgaga gcctcgcggt
     3061 cgcgctctat gcccgcgtcg gccttccggt gccggtcggc cgacccgccc ctcccggcgc
     3121 cccggcgacg ggttccgttc cgcaggggtc gggaccggaa gtcgtgttcg acgaattcga
     3181 aatcgagctt cccggcgccg gcgcgagtcc ggcggttgga gctcccgcgt tcgatccgtt
     3241 cgcggagctg cccgaggctt ccgcgtccgc tccgccgctc gcggcggcgg cgccggcagg
     3301 cgcctcctcc ccgggcgcgg cgcgcctgac tccccatgcc ccgggcgaca ccggcttcgg
     3361 actcctcgcc cccgagctcg gctccctgat cgaggagaag atgcaacgcg gcgcggccga
     3421 ggcgccaagg cgcgatctcc attcgccgcg tccgaaggtg gtggacgacg agaatctctt
     3481 tgctgacgag cggcagttct tcaacctcgc cgaggagctc gagaaggagc tctcggagga
//
LOCUS       DASWAV010000194         2898 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U2665
            k141_290119, whole genome shotgun sequence.
ACCESSION   DASWAV010000194 DASWAV010000000
VERSION     DASWAV010000194.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960316
            Sequence Read Archive: SRR10260007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2898)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2898)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:47:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,929
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2898
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_290119"
                     /isolate="SMAG_U2665"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.36444 N 132.31889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..783
                     /locus_tag="VF958_03915"
     CDS             <1..783
                     /locus_tag="VF958_03915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017462526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CocE/NonD family hydrolase"
                     /protein_id="HEX9148287.1"
                     /translation="ASKAQVAPVSAFETGTNTWRRLDSWPSGCASGCTVKPTPLYLAA
                     GARTSFSPPAAGSTDYEEYVSDPAKPVPFISRPVPPGWYEGNRAWRQWLVSDQREASG
                     RTDVVSFVSDVLKEPVKISGQPVANLIASTSGTDSDWVVKLIDVFPDEVATDPTMGGY
                     QLMVSGDIFRARYRESLETPKAIAANQPLLYRWQLPTANHVFLPGHRIMVQVQSSWFP
                     LYDRNPQTFVPNIMWAKPDDYRKAVQRVYSTSFVELPLVAGR"
     gene            780..1796
                     /locus_tag="VF958_03920"
     CDS             780..1796
                     /locus_tag="VF958_03920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011777920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016811 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear amides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetamidase/formamidase family protein"
                     /protein_id="HEX9148288.1"
                     /translation="MRAAGAALTIGLAAAIASAEQPVLRYVPKHEELKYTFGGVAPAA
                     RIKPGTRIVSWTEDCYDGAVTKADQTPSKVQLPGHDNPQTGPFYVEGAEPGDTLAIRI
                     EKLEPARDHGISSSFPGFGALNSTDRTALLHADLPETVWFYQVDSEKRVLRTRSKDGK
                     SAWEVPLSPFLGCLGVSPANGEARSTIVPDDFGGNMDCPEVRTGNTVYLGVRVPGALV
                     SFGDGHLGMGDGEIIGTAVEGAMNVELTVDLIKGRETPWPRIENDEWMMSVGAGRPLE
                     DAARVAFRDMVRWVREKTGLSEMDAYQFVSQSARAPIVQMVDPNYTVLVKIAKNRLPT
                     RPPG"
     gene            1838..2374
                     /locus_tag="VF958_03925"
     CDS             1838..2374
                     /locus_tag="VF958_03925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020713441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9148289.1"
                     /translation="MNKHWKTLLIAASIGGSVALAQEASGSCHDNGKKADSRHHAKVD
                     ERGDHVMGFDHSKTTHHFRLSRTGGSIEVSANDPQDAESRDAIRAHLAHVAGMFAEGN
                     FEAPMLIHDRVPPGVSTMKERKSRIEWKFEEMPDGGRVRITSKDPEALAAIHDFLRFQ
                     IEDHRTGDSTEISKDANR"
     gene            complement(2437..>2898)
                     /locus_tag="VF958_03930"
     CDS             complement(2437..>2898)
                     /locus_tag="VF958_03930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9148290.1"
                     /translation="VRVRGSFPLPHGRLSINIGAPVFPVGGYVPYGYNVYEDPEYGYG
                     FEYDNQWIPCQPQGTRWIVVERPYYGYGGDYRYSRPYRSDRYGYSRDYRRDNFRGYSR
                     DNRGYSRDNRSYSRDDRRYSRDNRSYSRDDRRSNDRDRRSRDGDGARRWRP"
BASE COUNT          544 a          932 c          957 g          465 t
ORIGIN      
        1 gcgtcgaagg cgcaagtcgc tccggtctcg gctttcgaga ccgggacgaa cacgtggcgg
       61 cgcctcgact catggccatc cggctgcgca tccggctgca cggtcaagcc gacgccgctc
      121 tatctcgccg ccggcgcgag aacgagcttt tccccgccag cggcgggaag cacggactac
      181 gaggaatacg tctccgatcc cgcgaagccc gtgccgttca tctcgcgccc cgtcccgccg
      241 ggctggtacg aaggcaacag ggcctggcgg cagtggctcg tctcggacca gcgcgaggcg
      301 tcgggccgga ccgacgtcgt ctcgttcgtt tcggacgtcc tgaaggagcc ggtcaagatc
      361 agcggccagc cggtcgccaa cctgattgcg tcgacgagcg gcaccgattc ggactgggtc
      421 gtgaagctga tcgacgtctt tcccgacgag gtcgccacag accccacgat gggcggttat
      481 cagctcatgg tctccggaga catcttccgc gcccgctacc gcgaaagtct cgagacgccg
      541 aaggcgatcg cagcgaacca gccgttgctc tatcggtggc agcttcccac ggcgaaccac
      601 gttttcctgc cgggccatcg gatcatggtg caggtccagt cgagctggtt ccctctttac
      661 gaccgcaacc cgcagacgtt cgttcccaac atcatgtggg cgaagccgga cgactaccgg
      721 aaggctgtcc agcgggtgta ctccacgagc ttcgtcgagc tgccgctggt ggcgggccga
      781 tgagagcggc cggggcggct ctcacgatcg gtctcgcggc ggcgatcgcc tccgcggagc
      841 agccggtcct tcgctatgtg ccgaagcacg aggagttgaa gtacacattc ggcggcgtgg
      901 cgccggccgc gcggatcaaa cccggcacgc gaatcgtcag ctggacggag gactgctacg
      961 acggcgccgt cacgaaggcg gaccagaccc cctcgaaggt ccagctcccc ggccacgaca
     1021 acccgcagac ggggcccttt tacgtggagg gcgcggagcc gggcgacacg ctcgcgatcc
     1081 gaatcgaaaa gctcgagccc gcgcgcgacc acggcatctc ctcgtcgttt cccgggttcg
     1141 gcgcgttgaa ctcgaccgac cgcacggcgc tgctgcacgc cgacctgccg gagacggtct
     1201 ggttctacca ggtcgattcc gagaagaggg ttctccgcac gcgatcgaag gacggcaagt
     1261 cggcgtggga agtcccgctg tctccgttcc tcggatgtct cggcgtgtca cccgcgaacg
     1321 gcgaggcgcg ctcgacgatc gtgcccgacg acttcggcgg caacatggat tgccccgagg
     1381 ttcgaactgg taacaccgtc tatctcggcg tgcgcgtgcc gggggcgctc gtctcgttcg
     1441 gcgacgggca cctcgggatg ggcgacggcg agatcatcgg cacggccgtc gaaggcgcga
     1501 tgaacgtcga gctcacggtc gacctcatca aaggccgcga aacgccgtgg ccgcggatcg
     1561 agaacgacga atggatgatg tcggtcggcg ccggtcgccc gctggaggat gcggcgcggg
     1621 tcgcgttccg ggacatggtc cgatgggtgc gcgagaagac cggactctcg gagatggacg
     1681 cctaccagtt cgtctcgcag agcgcgcggg cgccgatcgt ccagatggtg gaccccaact
     1741 acacggtcct ggtgaagatc gcgaagaacc ggctgccgac gcgtcctccg ggctgatcga
     1801 aagaatggcg gaacgccggc gaaagaaagg acgagaaatg aacaagcact ggaagacgct
     1861 cctgatcgca gcctcgatcg gaggctccgt tgctctcgcg caggaggctt cggggtcctg
     1921 ccacgacaac gggaagaaag ccgactcgcg tcatcacgcg aaagtggacg agcgcggcga
     1981 ccacgtcatg ggtttcgacc actcgaaaac gacccaccac ttccggctgt ctcggaccgg
     2041 cggctcgatc gaggtttcgg caaacgaccc gcaggacgcg gagagtcgcg acgcgatccg
     2101 ggctcatctc gcgcacgtcg cggggatgtt cgcggaggga aacttcgaag cgccgatgtt
     2161 gatccacgat cgcgtgcctc ccggcgtttc gacgatgaag gaacgcaaat cgcgaatcga
     2221 gtggaagttc gaggagatgc ccgacggcgg acgcgtgcgg atcacctcga aagatccgga
     2281 ggcgctcgcg gcaatccatg actttctgcg gttccagatc gaagaccatc ggaccggcga
     2341 ctcaaccgaa atttccaagg acgcgaatcg ctagagagcc aaaaaaaacc gccccgagag
     2401 gggcggtttt ccaaacgagt tcctggatcg aaggggtcac ggcctccagc ggcgggctcc
     2461 atccccatcg cgagaccggc gatcacgatc gttcgagcgc cggtcgtcgc ggctgtaact
     2521 gcgattatca cggctgtacc tgcggtcgtc acggctgtaa ctgcggttgt cacggctgta
     2581 accgcggttg tcacggctgt aaccgcggaa attgtcccgc cggtagtcgc gcgagtaccc
     2641 gtagcggtcg ctgcgatacg gtcgcgaata gcgataatcg ccgccgtagc cgtaataggg
     2701 acgctccacc acgatccagc gggttccctg cggctggcac gggatccact ggttgtcgta
     2761 ctcgaacccg tatccgtact cgggatcttc gtagacgttg tacccgtacg gaacgtaccc
     2821 gccgaccggg aagaccggcg cgccgatatt gatcgagagc cgtccgtgag gaagcggaaa
     2881 cgatccccga acccgaac
//
LOCUS       DASWAV010000195         2176 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U2665
            k141_730349, whole genome shotgun sequence.
ACCESSION   DASWAV010000195 DASWAV010000000
VERSION     DASWAV010000195.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960316
            Sequence Read Archive: SRR10260007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2176)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2176)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:47:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,929
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2176
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_730349"
                     /isolate="SMAG_U2665"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.36444 N 132.31889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..587)
                     /locus_tag="VF958_03935"
     CDS             complement(<1..587)
                     /locus_tag="VF958_03935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9148291.1"
                     /translation="MRSLWHKLDPRTSVASRLLLGLFLAFCIPGGAFVFLLERRLSEI
                     ERVSVRRLAGVRVSDATQRIEQDARFRTEWIDRGARLLEEAAASLAATVKLELTANEP
                     PPRISLTADPHGHVWTPSDEAGTVAWLVPERSSDTAARADLARTRMLAPLMRSLLERR
                     SSVRSVTVRTASGAARQAPWTDLHGAAFGPFASVSS"
     gene            complement(805..2022)
                     /locus_tag="VF958_03940"
     CDS             complement(805..2022)
                     /locus_tag="VF958_03940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011938054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX9148292.1"
                     /translation="MLKFLTILKVGLKAIARNKMRSALTALGIIIGVGCVIAMVGVGK
                     GSQAAIQSQINALGTNFLMVFPGVATQSGARIFTGESTLTEDDVAAVRAEAPSVAYVS
                     PMSRSGAQVVYGNSNWGTQVQGVGVEWPFIRSWNLSQGSFFGDNEVRSAAKVVVLGST
                     VANALFGDQNPVGASVRIKNFPFRVIGVLETKGGSTMGQDQDDTVVAPYTTVMRLLKG
                     RNRIDMFMASAVSQDAVEQAQNEIDSLLRQRHRLQPGQDADFMIRSQQEIAQTANETS
                     RTLSILLASAASISLLVGGIGIMNIMLVSVTERTREIGIRMAIGAKGRDILTQFLVEA
                     LTLSITGGLIGIALGVGASRLLAWKAKWSIALPPESILMAFGFSAAIGVFFGFYPARK
                     ASRLDPIEALRYE"
BASE COUNT          385 a          714 c          725 g          352 t
ORIGIN      
        1 gagctcacgg aagcaaaggg tccgaaggcg gcgccgtgca gatcggtcca cggcgcctgg
       61 cgagccgctc cggaagcggt tctgaccgtc acgcttcgaa cactcgagcg ccgctcgagg
      121 agagacctca tgagcggcgc caacattctc gtgcgagcga ggtccgcccg ggccgccgtg
      181 tccgatgacc gctctgggac gagccacgcc acggttccgg cttcgtccga aggagtccag
      241 acgtgaccgt gcgggtccgc cgtcagagag atccgcggcg gcggctcgtt cgctgtgagc
      301 tcgagcttca cggtcgcagc gagcgacgcg gccgcctcct ccagaagacg cgcgcctcgg
      361 tcgatccact ccgtgcgaaa ccgggcgtcc tgctcgatgc gctgagtcgc gtcggagacg
      421 cgcactccgg cgagccggcg gacggacacg cgctcgatct ccgagaggcg acgctccagg
      481 aggaagacga acgccccccc ggggatgcag aacgcgagga agaggccgag caggaggcga
      541 gaagccacgc tcgtgcgcgg atcgagcttg tgccacaagg atctcatcgt ggtcacggac
      601 ggaccccggc cccgccgccg cgttcgactc tcttcaccat ggattgaatc aagacatttt
      661 acagcgacgc cggcatgcgc gggtctcttt cgcgggcgcg aggagtctcc tgagtcgcgg
      721 cgggaggagt ctccgggaag tcaccgagcg gaggagcctc gccggtgcgg aacgcagatt
      781 ccggagcgaa gcgaccgcga cggtctattc gtagcggaga gcttcgatgg ggtcgagccg
      841 cgacgccttg cgtgccgggt agaacccgaa gaaaacgccg atcgcggcgg agaagccgaa
      901 cgccatgagg atcgactcgg gcggaagggc gatgctccac ttcgccttcc aggcgagaag
      961 ccgagacgcg ccgactccca gtgcgatacc gatcaacccg ccggtgatcg acagagtgag
     1021 cgcctcgacg agaaactgcg tgaggatgtc gcggcccttc gcgccgatcg ccatgcggat
     1081 cccgatctcc cgggtgcgct cggtcacgga gaccagcatg atgttcatga tcccgatccc
     1141 tccgacgagg agcgagatcg aggcggccga cgcgagaagg atcgacaggg tccgggacgt
     1201 ctcgttcgcc gtctgcgcga tctcctgttg cgaacggatc atgaagtcgg cgtcctggcc
     1261 gggctggagg cgatgcctct ggcggaggag cgagtcgatc tcgttctgcg cctgctcgac
     1321 cgcgtcctgc gagacggcgg acgccatgaa catgtcgatg cggttgcggc ccttcaggag
     1381 acgcatcacc gtcgtgtacg gtgcgacgac ggtgtcgtcc tggtcctggc ccatcgtgct
     1441 gccgcccttc gtctcgagca ccccgatcac gcgaaacgga aagttcttga tccgcacgct
     1501 cgcgccgacc gggttctgat cgccgaagag cgcgttcgcg acggttgagc cgagcacgac
     1561 gaccttcgcg gccgaccgca cctcgttgtc gccgaagaag ctgccctgcg agaggttcca
     1621 cgaacggatg aacggccact cgacgccgac gccctggacc tgcgtccccc agttcgaatt
     1681 gccgtagacg acttgcgctc cggatcgcga catcggcgag acgtacgcga ccgacggggc
     1741 ctccgcgcga acggcggcga cgtcgtcctc ggtcaacgtc gactcgcccg tgaagatgcg
     1801 cgctccggac tgggtggcga cgcccggaaa caccatcagg aagttcgtgc ccagagcgtt
     1861 gatctgagac tggatcgcgg cctgcgaacc cttgccgacg ccgaccatcg cgatcacgca
     1921 gccgacgccg atgatgatgc cgagcgcagt gagtgccgac cgcattttgt tgcgtgcgat
     1981 cgctttcaag cccaccttca gaatagtcag gaatttcagc atctcatgcc tccaccagct
     2041 tctgctcgtt tggccggtct tcgatgaccc ggccgtcgcg gaacgtcacg atccgcttcg
     2101 cgtgggccgc gatgtccgtc tcgtgcgtga tgagaacgat cgttttcccc tggtcgttca
     2161 agttctggaa cacggc
//
LOCUS       DASWAV010000196         3828 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U2665
            k141_530240, whole genome shotgun sequence.
ACCESSION   DASWAV010000196 DASWAV010000000
VERSION     DASWAV010000196.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960316
            Sequence Read Archive: SRR10260007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 3828)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3828)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:47:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,929
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3828
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_530240"
                     /isolate="SMAG_U2665"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.36444 N 132.31889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            159..977
                     /locus_tag="VF958_03945"
     CDS             159..977
                     /locus_tag="VF958_03945"
                     /inference="COORDINATES: protein motif:HMM:NF014826.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HEX9148293.1"
                     /translation="MRVDLHSHSSVSDGLDSPAELVFRMASAGVGAFALTDHDTLQGL
                     PEARAEAGRRGIELIPGAEISANFAGQDDVHILALFVDETNAAFNASLAERQAGRRTR
                     GERMAQKLIEAGFAIDLDAIREDVGDGVWGRPHLARALIRAGYAAGNDEAFDRFLGDD
                     QPWYVPYDKWEAEDVLDSVHRAGGICSLAHAIWYRDPEMLIAGLAAKGLDAVEVFHSD
                     HGPDEVERFGRLAARFGLGVSAGSDFHGSVEGRKAPGGVFGDEKMLERLRARRG"
     gene            974..1345
                     /locus_tag="VF958_03950"
     CDS             974..1345
                     /locus_tag="VF958_03950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9148294.1"
                     /translation="MTGAPPESWVVLIQRADEDALYEAAAMSASATSLGIAVTLVWFD
                     RALEALVSGRLDDTGERPASPSDLLASARDTGRLRLLACSASAVDPRTGVEKTRQKVD
                     EIVGWPTVIAMMRAATRAFVW"
     gene            1553..2908
                     /gene="dnaB"
                     /locus_tag="VF958_03955"
     CDS             1553..2908
                     /gene="dnaB"
                     /locus_tag="VF958_03955"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:NF004384.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HEX9148295.1"
                     /translation="MLEEIATEKGLPQNLDAERSVLGAILLDPASLASVFPILKEDDF
                     FPDTHRRIYRSMTDLAQRSAEIDVLTLKEDLDRSGSIEKAGGVAYLSALLDAVPDVAN
                     VEHYARIVKEKSTLRRLIRAGQRMVRDGMSQEKDAQGLLGDATGEIFDIAEDAVSGGF
                     ERIGEITRHTLDVIDEAKERSGAVSGLPTGFLELDRMTSGFQATDLIILAARPAVGKT
                     SLALNIAQHVAIREGRAVGFFSLEMSKEQIGFRVLCSEADVDAKKVRDGYASSEAITR
                     LVLAQSKVAGARFFIDDGSAVSVPEMRAKAQRLKREHGLDLLVVDYLQLMMGHGRFDN
                     RTQEVSQISRGLKLLAKELKVPILALSQLSRQPERRTGEMRRPQLADLRESGSLEQDA
                     DVVVFLYREELYDKDTERKGIADAIIAKQRNGPTGDFPLVFLADHMTFANYAGPALEP
                     F"
     gene            2956..>3828
                     /gene="alr"
                     /locus_tag="VF958_03960"
     CDS             2956..>3828
                     /gene="alr"
                     /locus_tag="VF958_03960"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HEX9148296.1"
                     /translation="MRPTRAAIDLDAIAANYRFIRERLGRDRAIYCVVKADAYGHGAG
                     PVARRLEREGASRFAVAIAEEGIVLRRAGVAAEILLLNYSDPADAGVHRAYGLVPSLY
                     DPAQAAAFAEATRGFHEPLPVHLKVDTGMGRIGFRLERIGPLIDLVKRSPHLLVAGVF
                     SNLSTADEPSSPATAKQVETMKAAVAAISSAGISPGVVHLANSAGILLHSDTWLDGVR
                     PGLALYGVCPTSDMPEERLVPAMTIETEVMAVNTIPANTPIGYGGRYVTRRVSQIAVV
                     PIGYHDGFRRSFSGR"
BASE COUNT          619 a         1274 c         1295 g          640 t
ORIGIN      
        1 gaccgcgtgc ctggtcgtcc gctgtggtcg agaatccgca tcgcgaagct cctccgcttg
       61 gttacttcgc ggagactctt cgcgatgcga acggaggaga cgccttcccg ttgctcagga
      121 gactcctcac gtcgcaacag agtaggatct cccccgtttt gcgcgttgat ctccacagcc
      181 attcgagcgt gtccgacggg ctcgactctc ccgcagagct cgtcttccgg atggcgtcgg
      241 cgggagtggg cgcgttcgcc ctcaccgacc acgacaccct ccagggactg cccgaggccc
      301 gcgcggaggc gggtcggaga gggatcgagc tcatcccggg ggcggagatc tcggcgaact
      361 ttgcggggca ggacgacgtc cacatcctcg cgctcttcgt cgacgagacg aacgcggcgt
      421 tcaacgcgag cctcgcggag cggcaggcgg gccgacggac gcgcggcgag cgaatggctc
      481 aaaagctcat cgaggcgggc ttcgcgatcg atctcgatgc gatccgcgag gacgtgggcg
      541 acggggtgtg gggacgcccc cacctcgcgc gggcgctcat ccgggccggg tacgcggccg
      601 ggaacgacga ggctttcgac cgttttctcg gtgacgatca accctggtac gtcccctacg
      661 acaagtggga ggcagaggac gtgctcgact cggtgcaccg ggccggcgga atctgctctc
      721 tcgcgcacgc gatctggtat cgcgatcccg agatgctcat cgccggtctc gcggcgaaag
      781 gcctcgacgc ggtcgaggtc ttccattcgg accacgggcc cgacgaggtc gagcgtttcg
      841 gaaggctcgc cgcccggttc gggctcggtg tcagcgccgg aagcgatttt cacggttccg
      901 tcgaaggtcg gaaggccccg ggcggggttt tcggcgacga gaagatgctc gagcggctcc
      961 gggcgcggcg cgggtgaccg gcgcccctcc cgagagctgg gtcgtcctca tccagcgggc
     1021 ggacgaggac gctctttacg aggcagcggc gatgtcggct tccgcgactt cgctcgggat
     1081 cgccgtcacg ctcgtctggt tcgatcgggc tctcgaggcg ctcgtctccg gccggctcga
     1141 cgacacggga gagcgtccgg cgagcccgtc ggatctcctg gcctccgcgc gagacaccgg
     1201 acggctccgt ctgctcgcat gctcggcgtc ggcggtggac ccgcgcaccg gagtcgagaa
     1261 aacgcgccaa aaggtggacg agatcgtggg ttggccgacg gtcatcgcga tgatgcgcgc
     1321 cgcgacacgg gccttcgtct ggtgaatcgc tccgcgacct cctgacaccg tcttctctcg
     1381 gacatcatct gtccccgccg ccgcctcatc cttgcggaaa aggagcggtg ctgcggaaaa
     1441 gaaatttttt cgttagcgcc gcacgcacgg aggtcgtccc cggatggatg cggaaatctt
     1501 tcgagcgttg acagaggcgg aggggggaag aagaatccgg ccgacctccc tgatgctcga
     1561 agaaatcgcg accgaaaagg ggcttccgca gaacctcgac gcggagagat cggtgctcgg
     1621 agcgatcctc ctcgatccgg cgtcgctcgc gtcggtcttc ccgatcctga aggaagacga
     1681 cttcttcccc gacacgcacc ggcgcatcta tcggtcaatg accgatctcg cgcagcgctc
     1741 ggcggagatc gatgtcctca cgctgaagga ggacctcgac cgctctggct cgatcgagaa
     1801 ggccggcggg gtcgcgtacc tttccgcgct gctcgacgcg gtgcccgacg tcgcgaatgt
     1861 cgagcactac gcgcgcatcg tcaaagaaaa gtcgacgctt cggcgcctga tccgcgccgg
     1921 acagcggatg gtgcgcgacg gcatgtccca ggagaaggac gcgcagggac tgctcggcga
     1981 cgcgacgggc gagatcttcg acatcgcgga agacgccgtt tccggcggct tcgagcggat
     2041 cggggagatc acccggcaca ccctggacgt catcgacgag gcgaaggaga ggagcggggc
     2101 ggtgtcgggg ctcccgaccg gcttcctcga gctcgatcgc atgacctccg gcttccaggc
     2161 caccgatctg atcatcctcg ccgcgcgtcc ggcggtcggc aagacctcac tcgcgctgaa
     2221 catcgctcag cacgtcgcca ttcgcgaggg acgggcggtc ggcttcttct ctcttgagat
     2281 gtccaaggag cagatcggtt tccgggtcct ctgctcggag gcggacgtgg acgcgaaaaa
     2341 ggtccgcgac ggctacgcct cgagcgaggc gatcacgcgg ctcgtgctcg cgcagtccaa
     2401 agtcgcgggg gcgcggttct tcatcgacga cggctcggcg gtgagcgtcc cggagatgcg
     2461 cgccaaggcg caacggctga aacgcgagca cgggctcgac ctgctcgtcg tcgactacct
     2521 gcagctgatg atggggcatg ggcggttcga caaccgcacg caggaagtct cgcagatttc
     2581 gcgcgggctg aagctgctcg cgaaagagct caaggtcccg atcctcgccc tctcgcagct
     2641 ctcgcgccag ccggagagac ggaccggcga gatgcgccgg ccgcagctcg cggatctccg
     2701 tgagagcgga tctttggagc aggatgccga cgtcgtcgtt tttctgtacc gcgaggagct
     2761 ctacgacaag gacacggagc gcaaagggat cgccgacgcc atcatcgcga agcagcgcaa
     2821 cggaccgacc ggcgactttc cgctcgtctt cctcgcggac cacatgacct tcgccaacta
     2881 cgcggggccg gcgctcgagc ccttctgacc tgcgcggctt cccgtcgaag cctctctctg
     2941 acatcctcgc acttcatgag accgacccgc gcggccatcg acctcgatgc gatcgccgcc
     3001 aactaccgtt tcattcgcga gcgcctgggg agggaccgcg cgatctactg cgtcgtcaag
     3061 gccgacgcgt acgggcacgg ggcgggaccc gtggcgcgcc ggctcgagcg cgagggcgcg
     3121 tcgcggttcg cggtcgcgat cgcggaagaa gggattgtgc tgcgccgcgc gggcgtggcg
     3181 gcggagatcc ttctgctcaa ctactcggat ccggccgacg ccggcgtcca ccgcgcgtac
     3241 ggtctcgtgc cgtcgctcta cgatccggcg caggcagccg cctttgccga ggcgacgaga
     3301 ggttttcacg agccgcttcc cgtccacctc aaggtcgaca cgggcatggg gcgcattggc
     3361 tttcgcctcg agagaatcgg ccccctgatc gacctcgtga agcgctcgcc gcacctcctc
     3421 gtcgccggcg tattcagcaa cctctcgact gccgacgaac cgagctctcc cgcgaccgcg
     3481 aagcaggtcg agacgatgaa ggcggccgtc gcggcgatct cttcggccgg gatttcgccg
     3541 ggtgtcgttc atctcgcgaa ctccgcgggg atccttctcc actccgacac ctggctcgac
     3601 ggcgttcggc cgggtctcgc gctctacggc gtctgcccga cgagcgacat gccggaggaa
     3661 cgactcgttc ccgcgatgac gatcgagacc gaggtcatgg cggtgaacac gattcccgcg
     3721 aatacaccga tcgggtacgg cgggcggtac gtgacgcggc gcgtttccca gatcgccgtc
     3781 gttccaatcg gctatcatga cggcttccgg cgctccttct cggggcgc
//
LOCUS       DASWAV010000197         1713 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U2665
            k141_251183, whole genome shotgun sequence.
ACCESSION   DASWAV010000197 DASWAV010000000
VERSION     DASWAV010000197.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960316
            Sequence Read Archive: SRR10260007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 1713)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1713)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:47:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,929
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1713
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_251183"
                     /isolate="SMAG_U2665"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.36444 N 132.31889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1588
                     /locus_tag="VF958_03965"
     CDS             <1..1588
                     /locus_tag="VF958_03965"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HEX9148297.1"
                     /translation="ELGPHAAGGDGGGGASARAGTRVREVMDRASPPFETQHKLLLLE
                     TLYDAGRSLGSLPSEDALVEDVLGRAVGVLDASRGYLATFGEGGARKAEARVGFPRRQ
                     PEGVVAGDEFLRDVLKSESAVIRERFRFLRTPVLEAIGVTIRAGGRPVGALVLADKEQ
                     RRGGRASFDDDDARFLTSLAGLSGMAIENRRHLERLTRERERLEEENRRLLDETGRSA
                     AGRLFIGDSPAARRVLDLVGRVASSKISVLLTGESGTGKELVARMIHARSDRADKAFV
                     PINCAALPEPLLESELFGIERGVATGVEPRLGKFEVANGGTIFLDEIGDMPLSLQAKL
                     LRVLQEREIERVGGRRRIPIDVRVLAATHARLPERIARGEFREDLYYRLRVVEIPLPP
                     LRERREDIPKLVRHFLERFAARDRREVAHLERDAFASLLAHDYPGNIRELENVLEGAA
                     ALSQGGVIHREDLQWLPSLGADPGERVDSNEASPANLRDLEERHVQRVLKLAGGNKSR
                     AARLLGISRRTLYRKKYLAH"
BASE COUNT          273 a          566 c          608 g          266 t
ORIGIN      
        1 cgagctgggc ccccacgcgg ccggcggaga cggcggtggc ggagcttcgg cgcgcgcggg
       61 tactcgcgtc cgcgaagtaa tggaccgggc gagccctccg ttcgaaactc agcacaagct
      121 ccttctcctc gaaacgctgt atgacgccgg gcgatcgctc ggctcgctcc cgagcgagga
      181 tgctctcgtg gaggacgtcc tggggcgtgc ggtcggcgtg ctcgacgcgt cccgcggata
      241 tctcgcgact ttcggtgagg ggggagcgcg gaaggcggag gcgcgggtcg gcttcccgcg
      301 ccgccagccg gaaggtgtgg ttgccgggga cgagttcctt cgcgacgtcc tgaagtcgga
      361 gagcgcggtc atccgagagc gcttccgatt cctgcggacg ccggttctcg aggcgatcgg
      421 agtcacgatt cgggccggcg ggcgtccggt cggcgctctc gtgctcgccg acaaggagca
      481 gaggcggggc ggacgggcgt ccttcgacga cgacgatgcg cgcttcctca cctcgctcgc
      541 cggcctgtcg ggcatggcca tcgagaaccg acggcacctc gagcgcttga cccgcgagcg
      601 cgagaggctc gaggaagaaa accgccgcct tctcgacgag accggccgct ccgcggcggg
      661 acgactgttc atcggcgatt cgccggcggc ccggcgtgtc ctcgacctcg tcgggcgcgt
      721 cgcgagctcg aagatctccg tgctgctgac gggggagagc ggcaccggca aggagctggt
      781 cgcccggatg atccatgcac gcagcgaccg cgccgacaaa gccttcgtgc cgatcaactg
      841 cgcggcgctc ccggagccgc tgctcgagtc ggagctgttc ggaatcgaac gcggggtcgc
      901 caccggtgtc gagccgcggc tcggcaagtt cgaggtggcc aacggaggga cgattttcct
      961 ggacgagatc ggggacatgc cgctgtcgtt gcaggcgaag ctgcttcggg tcctccagga
     1021 gagggagatc gagcgggtcg gggggcgccg ccggatcccg atcgacgtcc gcgtgctcgc
     1081 cgcgacccac gcccggctgc cggaacggat cgcgcggggc gagttccgcg aggaccttta
     1141 ctaccggcta cgcgtcgtcg agataccgct gccgcccctg cgcgagcggc gggaggacat
     1201 tcccaagctc gtgcgccact tcctcgagcg gttcgccgcg cgagaccggc gcgaggtcgc
     1261 tcacctcgag cgcgacgcgt tcgcatctct gctcgctcac gactaccccg gaaacatccg
     1321 cgagctcgag aacgtcctcg agggcgccgc ggcgctctcg cagggagggg tcatccaccg
     1381 cgaagacctt cagtggctcc cctccctcgg agcggatccc ggcgaaaggg tcgactcgaa
     1441 cgaggcgtcg ccggcgaacc tccgcgatct cgaggagcgg cacgtgcaac gggtgctgaa
     1501 gctcgcaggc ggcaacaaga gccgtgcggc gcggctcctc ggaatcagcc gacggactct
     1561 gtatcggaag aagtacctcg cgcactgagc gctcctcccg ggagttcgtg acattgtgtc
     1621 atcgtgacac ttagtcgcga tttcatggta agatccccat attcaaccga ttatccggaa
     1681 agccatttcg gaaaatctgg cgtacggcga caa
//
LOCUS       DASWAV010000198         5168 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U2665
            k141_131555, whole genome shotgun sequence.
ACCESSION   DASWAV010000198 DASWAV010000000
VERSION     DASWAV010000198.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960316
            Sequence Read Archive: SRR10260007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 5168)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5168)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:47:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,929
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5168
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_131555"
                     /isolate="SMAG_U2665"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.36444 N 132.31889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            122..511
                     /locus_tag="VF958_03970"
     CDS             122..511
                     /locus_tag="VF958_03970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015249452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HEX9148298.1"
                     /translation="MKTPALTHLSRRERQIMDVVYRLGRATATDVLAQMPEPPSYSAV
                     RAMLRVLENKGHLRHVLEGKSYVYLPTLARTRASRSALRNMLQTFFDGSTEKAVAALL
                     DLSRADLTDDEFARLSNLIDHARQEGR"
     gene            513..3011
                     /locus_tag="VF958_03975"
     CDS             513..3011
                     /locus_tag="VF958_03975"
                     /inference="COORDINATES: protein motif:HMM:NF017390.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M56 family metallopeptidase"
                     /protein_id="HEX9148299.1"
                     /translation="MTENLAIAVRFALDVAFKATILFAATAAALFALRRTSAAVRQFV
                     ATLGLAGVLALPAVSLFAPRWEIPLIPNPVPLAVPAVSELTAPRAEARPTSRSDWPAT
                     SEAAADTEPSLRPPDVVSSRSNLASTSTTKDFEPDGGTPKTPSSPTWWMFGALALWVG
                     GTLLASARLVLGANRVAAIRRRASEVDENWTAVSYELGAKLGVARPVRLLFSRDVAIP
                     VTAGLRRPVVLLPDAARRWNEERRRVVLLHELAHVRRCDWMALLVGQAAVALYWFHPL
                     AWSIRVQMRKDCERACDDLVLAAGTRASVYAAHLLSIIRSLRLSRQRALPAVAMAHRS
                     YWDGRMRAILDPAVARRVVTGREAKVAAAAIFTAVAALAVFEPWAPACAAANGSEHVR
                     PAVGRSVSGKAAGPHADSECPIEREKKAKQASLVASVVGEVAANVAGEVAANVIDEVA
                     TGTATWQDEGFTKAGWKQHKSGGDWYSRGMDLHNEGRYDEAIGAFGHAIEEGHRVDAA
                     MYNIACGYARKGDTDRAVEWLRKSLHEGFDLASYIDRDDDLDGIRSDPRFVELRRNLR
                     TEKIAANRNRADRLALRFVELKDSRSTTGTAFYAIGKELLDVGRYDVAAQAFQASAAR
                     GYREGVSLYNTACALSLAGDRPGALDYLEKALNAGFDDVGLLRKDDDLDNVRGDPRYG
                     ELLKMADDLELHRSDHGPDVKSWARSVRRSVLRETEQHFLDYAQRHPQSGRAWFNLGY
                     ARIQGERPEAAAEAFQRALDLRYRPATTMYNLACAYSLMDRKDDAFAWLFKALDAGFD
                     SSGMLRGDDDLDNLRGDPRYRQALARAKALGSED"
     gene            3243..>5168
                     /locus_tag="VF958_03980"
     CDS             3243..>5168
                     /locus_tag="VF958_03980"
                     /inference="COORDINATES: protein motif:HMM:NF012550.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitase family protein"
                     /protein_id="HEX9148300.1"
                     /translation="MHRIEPARLKPGVTEDRLPGPRPSDSAGEGPPRTIRGRALVFWD
                     PKAPGRKRDAIDTDQITPAADCVSESIEKLDERWKAGSFRYLMPDFRERVHRGENFLV
                     AGDRFAIGSSREMSPAGLKGVGEEAGLELVVVCGANMGDIFRRNAFNLGLHVVQSPDA
                     VADAQDGDEFSFDPATRGLTNETRGKSYEPVPLTPKEEEIRRSGGIFAVGRREFRRSV
                     ETLPSVTWPDPETARRMTTTEQIVWAHRVDKDARVAPGATLRVYADLLPASDGTAPFA
                     IHTFNQITGGDAIFPRQLAIANDHFVFTGKDSDERQTSIGREFARRHGIEKPYYATPG
                     DGIFHFYFPEQGLVLPGQFIPGADSHSRAYGAYGAVGFGVGSTTLGFGWSTGYIYFTL
                     AKQRRVLFRGTLRPWVSGKDIVLELLRRWGAKQSGGMSVEFVDVEKQLPIAYRNTIAN
                     MMAEAEALNGIFAQDELTDAWYRAKGIAPETLPYPRLAPGADAVYEIDEELSLDGVEP
                     MIAKPYSPGNAFPADEVARERLPFDKALIGSCTNGSYDDLLSAALVFRAAREQGFGKV
                     AKSLAVFPGSGGVGREIEVPDPRLGGESIAQVFRSVGGEIRQSWCGPCFGQGPDALSK
                     GERAITSFNRNWQNRMGL"
BASE COUNT          825 a         1780 c         1706 g          857 t
ORIGIN      
        1 cccaaagccc ggagaacctt tttgcggcct ccccgtatcc cttgacgatc tgatgctgtt
       61 tctttagcat gtgctggttt tttagcagtt gaaccgacgc gaccgatcga agggaggttc
      121 cttgaagacc ccggctctca ctcacctttc gcggcgggag cgtcagatca tggatgtcgt
      181 ctaccggctg gggcgggcga ccgccacgga cgtgctcgcg cagatgcccg aacccccgag
      241 ctactcggcc gtccgcgcga tgctgcgagt cctcgagaac aagggacacc tccgccacgt
      301 cctggagggc aagagctatg tctacctccc gacgctcgcc cggacccgcg cgagccgctc
      361 cgcgctccgc aacatgttgc agaccttctt cgacggctcg accgagaaag cagtcgcagc
      421 gctcctcgac ctttcgcggg ccgacctgac cgacgatgag ttcgcgcgtc tttccaatct
      481 gatcgaccac gccaggcagg agggccgctg aaatgacgga gaacctcgcc attgccgtcc
      541 gttttgccct cgacgtcgcc ttcaaggcta cgatcctctt cgcggccacg gcggcggcgc
      601 tcttcgcctt gcggcgcacc tcggcggccg tccggcagtt cgtagcaacc ctcgggctcg
      661 ccggcgtgct cgcgctgccc gcggtgtctc tcttcgctcc gcgatgggag atcccgctga
      721 tcccgaaccc tgtcccgctg gcagtccccg ccgttagcga actcaccgcc cctcgggcgg
      781 aagcgcgtcc aacctcacgg tcggactggc ccgcgacttc ggaggccgcc gccgacaccg
      841 agccgtcgct ccgccccccg gacgtcgtct catcgagaag caaccttgct tcgacctcga
      901 caacaaagga tttcgagccg gacgggggca caccgaagac gccctcctct ccgacgtggt
      961 ggatgttcgg ggctctcgcg ttgtgggtgg gtggaactct tctcgcttcg gcacgtctcg
     1021 tccttggcgc gaaccgcgtg gcggccattc gccggcgtgc gtcggaagtg gacgagaact
     1081 ggaccgccgt ttcctatgag ctgggcgcga aactcggcgt ggcgcgccca gtccggctcc
     1141 ttttcagcag agacgtcgcc attcccgtca cggcgggcct tcgtcgtccc gtcgttctcc
     1201 tccccgacgc ggcccggaga tggaacgaag aacgccgccg cgtcgtcctg cttcacgagc
     1261 tcgcgcacgt gcgacgctgc gactggatgg ctcttctcgt cggacaggcc gcggtcgctc
     1321 tctactggtt tcatccgctc gcgtggtcca ttcgcgttca gatgcggaag gactgcgagc
     1381 gggcgtgcga tgacctggtc ctcgcggccg gcacgcgggc ttcggtctat gctgcgcacc
     1441 ttctctcgat cattcgttcg ctgcgcctgt cccggcagcg tgcgttgccc gccgtcgcca
     1501 tggcgcaccg ttcctactgg gacggccgca tgcgcgccat cctggatccg gcggtcgcgc
     1561 gccgagttgt caccggtcgc gaggccaaag tcgccgcggc cgcgattttc accgccgtcg
     1621 cggcgctggc ggtcttcgag ccgtgggcgc cggcctgcgc tgcggcgaac ggctccgagc
     1681 acgtccgccc ggcggtcggc cgatccgtca gcggcaaggc cgctggaccc cacgccgact
     1741 cggagtgccc gatcgaacgt gaaaagaagg cgaaacaagc ctcgctagtc gccagcgttg
     1801 tcggtgaagt cgccgccaac gttgccggtg aagtcgccgc caacgtcatc gacgaagtcg
     1861 ccaccggcac cgcgacgtgg caggacgagg gtttcacgaa ggccggctgg aagcagcaca
     1921 agagcggcgg tgactggtat tcccgcggca tggatctcca caacgagggg cgctacgacg
     1981 aggcgatcgg tgctttcggc cacgcgatcg aagagggcca ccgcgtcgac gccgccatgt
     2041 acaacatcgc atgcggctat gcgcgaaaag gcgacacgga ccgcgcagtc gagtggctcc
     2101 ggaagtcgct ccacgagggc ttcgatctcg cttcttacat cgacagggac gacgacctgg
     2161 acggaattcg gtccgatccg aggttcgtcg agctccggcg caacctgcga accgagaaaa
     2221 tcgcggcgaa ccggaatcgg gcggatcgtc tcgccctgcg tttcgtcgag ctcaaggact
     2281 cgcgctccac gacgggcacc gcgttctacg caatcggcaa ggagctcctc gatgtcggcc
     2341 gatacgacgt cgccgcgcag gcgttccagg cgtcggccgc gcgcggatat cgcgaggggg
     2401 tgtcgctcta caacaccgcc tgcgcgctct cgctcgcggg agaccgtccg ggtgctctcg
     2461 attacctgga aaaggcgctc aacgcgggtt tcgacgacgt cggtctcctc cgaaaagacg
     2521 atgacctcga caacgtccga ggcgatccgc gctacggcga gctcctgaag atggcggacg
     2581 acctcgagct ccaccgctcg gaccacggcc ccgacgtcaa gtcgtgggcg cgctccgttc
     2641 gccgttccgt gttgcgcgag accgaacagc actttctcga ttacgcgcag cgtcacccgc
     2701 agagcggccg cgcctggttc aacctcggct acgcccgcat ccagggggag cggccggagg
     2761 cggcggccga ggcgttccag cgagctctcg acctgcgcta tcgccccgcg acgacgatgt
     2821 acaacctcgc ctgcgcttac tcgctcatgg atcggaagga cgacgccttc gcgtggctgt
     2881 tcaaggcgct cgacgcggga ttcgactcgt ccgggatgct ccgcggcgac gacgacctcg
     2941 acaatctgcg cggtgaccct cgctaccgcc aggcgctcgc ccgcgcgaag gccctcggca
     3001 gcgaagacta acccccgtct cttgctggtc gagccggcgc gcctctcgct ctctcgtcgg
     3061 tccggcgcga tcggaacgtc gattgcgaag tgttgtaccg agcgtcggac cgcgtcagga
     3121 cccagcttcg cgcagaggtt cgtctcttca tcgggcgggg agagcgcgag actcggggcg
     3181 ggggagccct ctcgattccg gtcagaaatg cggcggaccg cgaaagagtt agggttatcc
     3241 cgatgcatcg gatcgaaccc gcccgtctga aacccggcgt caccgaagac cggcttcccg
     3301 gcccccgtcc gtcggactcc gcgggcgaag gcccgccgcg cacgatccgc ggccgggcgc
     3361 tcgtcttctg ggacccgaag gcgcccggca ggaaaaggga cgcgatcgac acggaccaga
     3421 tcacgcctgc agccgactgc gtctcggaga gcatcgagaa gctcgacgag cgctggaagg
     3481 cgggctcgtt ccgctacctg atgccggact tccgcgagcg ggtgcaccgc ggcgagaact
     3541 tcctcgtcgc cggagaccgt tttgcgatcg gctcgtcccg cgagatgagc cccgcgggat
     3601 tgaagggcgt cggcgaagag gccgggctcg agctcgtcgt cgtctgcggc gccaacatgg
     3661 gcgacatctt ccggcgcaac gctttcaacc tcggcttgca cgtcgtgcag agccccgacg
     3721 cggtcgccga cgcgcaggac ggggacgagt tctcgttcga ccccgcgacg cgcgggctga
     3781 cgaacgagac gcgggggaag agctacgagc cggtgcctct cactcccaaa gaggaggaga
     3841 tccggaggag cggcggcatc ttcgccgtcg ggcggcgcga gttccgccgg tcggtcgaaa
     3901 cgctgccgtc cgtgacatgg cccgatccgg aaacggcgcg acggatgacg acgaccgagc
     3961 agatcgtctg ggcccaccgc gtcgacaagg acgcgcgcgt cgcacccgga gcgacgcttc
     4021 gcgtgtacgc ggatctcctc ccggcttcgg atggaacggc gccgttcgcc atccacacct
     4081 tcaaccagat cacgggcggt gacgcgatct ttccccggca gttggcgatc gcgaacgatc
     4141 acttcgtctt caccggcaag gattcggacg agcgtcagac gtcgattggc cgcgagttcg
     4201 cgcgccggca cgggatcgag aagccgtact acgcgacacc cggagacggg atcttccact
     4261 tctactttcc cgagcagggc ctcgtgctgc ccggccagtt catcccgggc gcggactcgc
     4321 acagccgtgc ttacggcgca tacggcgccg tcgggttcgg cgtcggctcc acgacgctcg
     4381 ggttcggctg gtcgacgggc tacatctact tcacgctcgc gaagcagcgg cgtgtcctct
     4441 tccgcggaac tctccgcccg tgggtgagcg gcaaagacat cgtgctcgag ctcctgcgac
     4501 ggtggggcgc gaagcagtcg ggcggcatgt ccgtcgagtt cgtggacgtg gagaagcagc
     4561 ttccgatcgc ctaccggaac acgatcgcga acatgatggc ggaggcggag gcgctcaacg
     4621 ggatcttcgc gcaggacgag ctcacggacg cctggtaccg cgcgaaaggg atcgccccgg
     4681 agacgctgcc ttatccgcgc ctcgcgcccg gcgccgacgc cgtctacgag atcgacgaag
     4741 agctctctct cgacggggtc gagccgatga tcgcgaagcc gtactcgccg ggtaacgcgt
     4801 ttcccgccga cgaggtcgcg cgcgagaggc tccccttcga caaggcgctc atcggctcct
     4861 gcacgaacgg cagctacgac gaccttctct ccgcggcgct cgtcttccgc gccgcgcgcg
     4921 agcagggttt cgggaaagtg gcgaagtcgc tcgcggtatt tccgggctct gggggcgtcg
     4981 gaagagagat cgaggtgccg gacccgcgcc ttggcggcga atcgatcgct caggtcttcc
     5041 gctccgtcgg cggggagatc cgccagtcgt ggtgtggccc ctgcttcggg caggggccgg
     5101 acgcgctgtc gaaaggcgag cgggcgatca cgtcgttcaa ccggaactgg cagaaccgaa
     5161 tgggcctg
//
LOCUS       DASWAV010000199         2794 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U2665
            k141_210936, whole genome shotgun sequence.
ACCESSION   DASWAV010000199 DASWAV010000000
VERSION     DASWAV010000199.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960316
            Sequence Read Archive: SRR10260007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2794)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2794)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:47:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,929
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2794
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_210936"
                     /isolate="SMAG_U2665"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.36444 N 132.31889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..139
                     /locus_tag="VF958_03985"
     CDS             <1..139
                     /locus_tag="VF958_03985"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013452101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HEX9148301.1"
                     /translation="WKRGAGVKDSGNFLPGHGGFYDRIDSLLYAAPVLAGFVATLNVS
                     V"
     gene            150..1322
                     /gene="dxr"
                     /locus_tag="VF958_03990"
     CDS             150..1322
                     /gene="dxr"
                     /locus_tag="VF958_03990"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011422631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0030604 - 1-deoxy-D-xylulose-5-phosphate
                     reductoisomerase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HEX9148302.1"
                     /translation="MKSVSVLGSTGSIGRAALDVIGSLPDRFRVAALAAGRSLEKLAA
                     QIERHAPSLVSIAREEDVAPLRSLLPAGFGGRIVAGPEGLEQAATLPDADIVLAGLVG
                     AVGLRSAHAALAAGKRLALANKEALVVAGELMVTTARASGAEILPVDSEHSAVHQAIR
                     SGRPEEVQRILLTGSGGPLRERDLSTFATITVEEALAHPTWKMGPKISIDSATMMNKG
                     LEIIEAHFLFGIAPSKIDVVIHPQSVIHSFVEFVDGSLVAQIARNDMRFPILYALAYP
                     ERLPNPFGRLDLVGLSRLEFQEVEPRRYPALGVARAALEAGGGMPAVVNAANEAAVEA
                     FLEGKISFPDIVAVVSETAAAHGRVPAPATVEEAENIDREARRSASRILRTLARAS"
     gene            1347..2699
                     /gene="rseP"
                     /locus_tag="VF958_03995"
     CDS             1347..2699
                     /gene="rseP"
                     /locus_tag="VF958_03995"
                     /inference="COORDINATES: protein motif:HMM:TIGR00054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HEX9148303.1"
                     /translation="MTILGNTLALLVVLSVLVVIHEFGHFAVAKAFRFPVEVFSFGFG
                     KRLFGKTWNGTDYRVSAIPLGGYVRVVGLGPDESTLAEGSSTEAAPVGKRWQRALILL
                     AGPAMNLVLAFVLHSVVFAVGTEVPAYQLEAPVVRMVESGSAGATAGLQVGDRILSVG
                     GTKTDRWRDAEFAFGMNAREPLEVRFLRGNREMTAVVTPRAVGKYDLGDVGLYGLQVR
                     PKIGLVSSGYPAETAGLRRGDVILEVGGHRIEGSPTQTFAMFVKAVESGAPGPIPIKI
                     LRGGAERTVSVSPKREGDSWKVGVQVMPDVPEIVERFPLGQAFVEGWRRVETDFRMTL
                     SVIGRLFRGKASMRSMSGPLDIAKFSGEAARTGAVPLLALMAAISLQLGIFNALPIPV
                     LDGGHLFLLSLEGIARRDFSLKVKERILQVGFLMILALITVVLYNDLAKNLPAKWWPF
                     "
BASE COUNT          399 a          964 c          966 g          465 t
ORIGIN      
        1 ctggaagcgt ggcgcgggcg tgaaggactc ggggaacttc ctgccgggac acggcggttt
       61 ctacgaccgg atcgattcgc ttctctacgc ggcgccggtc ctggcggggt tcgtcgcgac
      121 gctgaacgtc tccgtataat cgaaactccg tgaaatccgt ctccgtattg ggctcgaccg
      181 ggtcgatcgg gcgcgccgcg ctcgacgtga tcggctccct ccccgatcgg ttccgggtcg
      241 cggcgctcgc cgcgggacgc tcgctcgaga agctcgcggc gcagatcgag cggcatgcgc
      301 cgtccctcgt ctcgatcgcg cgcgaagagg acgtcgcgcc gctccgatcg ctccttccgg
      361 cggggttcgg cggccggatc gtcgccggtc ccgagggcct cgagcaggcg gccactctcc
      421 ccgacgccga catcgtgctg gcgggcctcg tcggggcggt ggggctccgg tcggcacacg
      481 cggcgctcgc ggccggcaag cgcctcgcgc tcgcgaacaa ggaggctctc gtcgtcgccg
      541 gcgagctgat ggtgacgacc gcgcgagcga gcggagcgga gattctgccg gtcgattcgg
      601 agcacagcgc cgtccaccag gcgatccgca gcggtcggcc cgaggaggtc cagcggatcc
      661 tgctcaccgg ctcgggcgga ccgctgcgcg agcgggacct ctcgacgttc gcgacgatca
      721 cggtcgagga ggcgctcgcc cacccgacct ggaagatggg cccgaagatc tcgatcgatt
      781 ccgcgacgat gatgaacaag gggctcgaga tcatcgaggc acactttctc ttcgggatcg
      841 cgccctccaa gatcgacgtc gtcatccacc cccagtcggt catccactcg ttcgtcgagt
      901 tcgtggacgg ctccctcgtc gcccagatcg cccgaaacga catgcgcttc ccgatcctct
      961 acgcgctcgc ctaccccgag cgcctgccga accccttcgg gcgactggac ctggtcgggc
     1021 tctcgcgcct cgagttccag gaggtcgaac cgcggcggta tccggcgctc ggcgtggccc
     1081 gagcggccct cgaggccggg ggcggcatgc cggcggtcgt caacgccgcc aacgaggcgg
     1141 cggtcgaagc cttcctggag gggaagattt cgttccccga catcgttgcg gtggtgtccg
     1201 agactgccgc cgcccacggc cgcgtgcccg cgccggcgac cgtcgaggaa gccgagaaca
     1261 tcgatcgcga ggcccggcgt tcggcttcgc ggatcctccg cacgctcgct cgtgcgagct
     1321 gaagggaaaa gtcgagaaaa ccgaccatga ccatcctggg caacacgctc gcgctcctcg
     1381 tcgtcctttc cgtcctcgtg gtgatccacg agttcggaca cttcgcggtc gcgaaggcgt
     1441 ttcgcttccc cgtcgaggtt ttttccttcg ggttcggaaa gcgcctcttc ggcaagacgt
     1501 ggaacggcac ggactatcgc gtttccgcga tcccgctcgg cggttacgtg cgggtcgtcg
     1561 ggctcgggcc cgacgagtcg acgctggcgg aggggagctc caccgaggcg gctccggtcg
     1621 ggaaacgctg gcagcgcgcg ctgatcctcc tcgccgggcc ggccatgaac ctcgtcctcg
     1681 ccttcgtgct ccacagcgtc gtgttcgccg tcggcaccga agtccccgcc taccagctcg
     1741 aggccccggt cgtccggatg gtcgagtcgg gctcggcggg cgccacggcg ggcctccagg
     1801 tcggcgaccg gatcctgtcg gtcggcggca cgaagacgga ccgttggcgc gacgcggagt
     1861 tcgccttcgg gatgaacgcg cgcgagcccc tcgaggtgcg gttcctccgg gggaacagag
     1921 agatgaccgc ggtggtgacg ccgcgcgccg tcgggaagta cgacctcggg gacgtcgggc
     1981 tctacggcct ccaggtccga ccgaagatcg gtctcgtctc gagcggttat ccggcggaga
     2041 cggccgggct gcgacggggc gacgtcatcc tcgaggtcgg cgggcaccgg atcgaaggat
     2101 cgccgacgca gacgttcgcg atgttcgtca aggcggtcga gtccggagcg ccgggcccga
     2161 tcccgatcaa gatcctccgg ggcggcgccg agcgcaccgt gtccgtctcg cccaaacgcg
     2221 agggagactc gtggaaggtc ggcgtccagg tgatgccgga cgtgcccgag atcgtcgagc
     2281 gctttcccct cggacaggcg ttcgtggaag gctggcggcg cgtcgagacg gacttccgga
     2341 tgacgctgtc ggtgatcggg cggctgttcc gcggcaaggc gagcatgcgc tcgatgtcag
     2401 gcccgctcga catcgcgaag ttctcgggag aggcggcgcg cacgggcgca gtgccgctcc
     2461 tcgccctgat ggcggcgatc tcgctgcagc tcgggatctt caacgccctt ccgatcccgg
     2521 tcctcgacgg cggacatctg ttcctgctct cgctcgaagg gatcgcgcgg cgggacttct
     2581 ccctcaaggt caaggagcgg atcctccagg tcgggttcct catgatcctc gcgctcatca
     2641 cggtcgtcct gtacaacgac ctcgcgaaga acctgcccgc caaatggtgg cccttctaac
     2701 gctttatccc ctctctccgc tcgcggggag agggtcgggg tgaggggatt acgataagcc
     2761 cgatgcccca gctccccgtc ctgaatgcac cccc
//
LOCUS       DASWAV010000200         2711 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U2665
            k141_650616, whole genome shotgun sequence.
ACCESSION   DASWAV010000200 DASWAV010000000
VERSION     DASWAV010000200.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960316
            Sequence Read Archive: SRR10260007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2711)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2711)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:47:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,929
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2711
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_650616"
                     /isolate="SMAG_U2665"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.36444 N 132.31889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..952)
                     /locus_tag="VF958_04000"
     CDS             complement(<1..952)
                     /locus_tag="VF958_04000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9148304.1"
                     /translation="MNRPRTLSFSAAFLFALAFPLSADTGHDHSEGDPTKLGKVHFPI
                     SCSAESQKKFDAGLAMLHSFWYGKSDKAFADLAAADPGCAMAHWGVAMSRFHQIWEVP
                     PPDDLKAGVAAIAAARAASAKTQRERDYIEALGAYYDKSDTLDHVTRFLAYEKAMEAL
                     AAKYPKDPEASIFYALALNGVANNGLPPDKTYARQKKAGAILEPIFKEMPEHPGIAHY
                     IIHSYDYPPLAERGLDAARRYAKIAPDSPHALHMPSHIFTRLGLWDDSIASNLASAEA
                     AHRDFQRTMPGGFSFDELHAMDYLAYAYLQTGRDGEARKIL"
     gene            1031..>2711
                     /locus_tag="VF958_04005"
     CDS             1031..>2711
                     /locus_tag="VF958_04005"
                     /inference="COORDINATES: protein motif:HMM:NF014896.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HEX9148305.1"
                     /translation="MTWGIALGVAISLLGGPTTTTASPVPPAARRESKVDVVHGEKRA
                     DEYFWLRRKEDPEVRAYLEAENAYTDLVMKPTVPLQEALYSELLGRIKETDLTVPYRK
                     GDFFYYSRTEKGKQYPILCRKRGSLDAAEEIYLDENDLAKGEKFFAIGDLEVSDDGNL
                     LAYTTDVTGFREYTLFVKDLRTGGVSPERVAKVSSIAWAADNRTLFYVVDDSAKRPYR
                     LYRHTLGADSASDPLLYEERDEKFVLDVGRTRSGELVLADSSSHTASEIRYLSAREPA
                     GKLKVLAVREKDREYDVDHRGELFYIRVNDRGRNFRLVTAPASDPRPENWKEIVPHRD
                     DVMLEGMDVFARHLVIFEREAGLRRFVVTDLETGKSHRIEFPEPTYAVFPDDNRVFDT
                     ATFRFRYQSLVTPPSVFDYELGTRERKLLKQTEVLGGYDPSKYVSERLAARAADGTAI
                     PISIVYGKSVKRDGRSPMLLTGYGSYGVSSNPTFSSNRVSLLDRGVVCAIAHVRGGGE
                     MGKKWHDQGKMFAKKNTFTDFISVAEYLVHEKWTSPDRLVIEGGSAGGLLVG"
BASE COUNT          499 a          839 c          890 g          483 t
ORIGIN      
        1 ccagaatttt ccgagcctct ccgtcgcggc cggtctgcag gtatgcatac gcgagatagt
       61 ccatggcgtg aagctcgtcg aatgagaaac ctccgggcat cgtccgctgg aaatcccggt
      121 gcgcggcctc ggccgaagcg aggttcgacg cgatggaatc gtcccagagc ccgagcctcg
      181 tgaagatgtg tgaaggcata tgaagcgcgt gcggcgagtc gggagcgatc ttcgcgtagc
      241 gcctcgcggc atcgaggccc cgctccgcca gcggcggata gtcgtagctg tggatgatgt
      301 agtgggcgat tccgggatgc tccggcatct ccttgaagat cggctcgagg atcgcgcccg
      361 ccttcttctg tcgggcgtag gtcttgtccg gcggcaggcc gttgttcgcc acgccgttca
      421 gcgcgagggc atagaagatc gacgcctccg ggtccttcgg atatttcgcg gccagagcct
      481 ccatagcctt ctcgtaggca aggaaacgcg tcacgtgatc gagcgtgtcg gacttgtcgt
      541 aatacgcccc gagcgcctcg atgtagtcgc gctctctctg ggtcttcgcg gaggcggccc
      601 gcgccgccgc gattgcagcg actcccgcct tgaggtcgtc gggaggaggc acctcccaga
      661 tctggtggaa gcggctcatc gcgacgcccc agtgcgccat cgcgcagccc gggtcggccg
      721 ccgccaggtc ggcgaaagcc ttgtcggact tgccgtacca gaaggagtgc agcatcgcga
      781 ggccggcgtc gaatttcttc tgcgactccg cgctgcagga gatcggaaag tgaacctttc
      841 cgagcttcgt cggatccccc tcggagtggt cgtgcccggt gtcggccgac aggggaaacg
      901 cgagtgcgaa gaggaacgcc gcggagaacg aaagtgttct tggtcgattc atcgggacct
      961 ccggaatggc ggattctaaa ccgagccggg agttatgatc ggccgctttt ggagcctatg
     1021 aggtgccgtc atgacatggg ggattgccct cggggtcgcg atcagtctgc tcggcggccc
     1081 gacgactacg accgcttccc cggttccacc ggccgcgcgg cgggagtcta aggtggacgt
     1141 cgttcacgga gagaagcgcg ccgacgagta cttctggctg cgccggaaag aggacccgga
     1201 ggttcgcgcg tacctcgagg cggagaacgc ctacacggat ctcgtgatga agccgaccgt
     1261 gccgctccag gaggctctct actccgagct gctcgggcgc atcaaggaga cggacctcac
     1321 ggtcccgtat cgcaagggcg actttttcta ttactcgcgc acggagaagg gaaaacagta
     1381 cccgatcctc tgccgtaaac gaggaagcct cgacgccgcc gaagagatct acctcgacga
     1441 aaacgatctc gcgaaggggg agaagttttt cgcgatcggc gacctggaag tcagcgacga
     1501 cggcaacctt ctcgcctata cgacggacgt gacggggttc cgggagtaca cgctgttcgt
     1561 caaggacctt cgcaccggcg gggtcagccc cgagcgagtc gcgaaagtca gctcgatcgc
     1621 ctgggcagcc gacaatcgga ccctcttcta tgtcgtcgac gactctgcga aacgtccgta
     1681 ccgactctac cggcacacgc ttggtgcgga ttccgcttcg gatcctctct tgtatgaaga
     1741 gagggacgaa aagttcgtgc tcgacgtcgg acgcacgcgg agcggcgagc tcgttctcgc
     1801 cgactcgagc agccacaccg ctagcgagat tcgttacctg tccgcccggg aacccgccgg
     1861 caagctcaag gtgctggcgg tccgcgagaa agaccgcgaa tacgacgtgg atcaccgggg
     1921 cgagctcttt tacatccggg tgaacgaccg cgggcgaaac ttccggctcg tcacggcgcc
     1981 ggcgtccgat ccacgccccg agaactggaa ggagatcgtc ccgcaccgcg acgatgtgat
     2041 gctcgagggg atggacgtct tcgcccggca cctggtgatc ttcgagcgcg aggccggatt
     2101 gaggcggttc gtcgtcacgg atctcgaaac tggaaaatcg catcgcatcg agttccccga
     2161 accgacctat gcggttttcc ccgacgacaa tcgcgtcttc gacacggcca cgttccgctt
     2221 ccgataccag tcgctcgtga cgcctccgtc cgtgttcgat tacgagctcg ggacgcgtga
     2281 acggaagctc ctcaagcaga ccgaggtgct cggcggctac gatccgtcga agtacgtctc
     2341 ggagcgcctg gctgccagag ccgccgacgg cacggcgatc ccgatctcga tcgtctacgg
     2401 gaagagcgtc aaacgcgacg gccggagccc gatgctcctg accggctacg gctcctacgg
     2461 agtctcgagc aatccgacct tctcgtcgaa ccgcgtgagc ctgctcgacc gcggcgtcgt
     2521 gtgcgcgatc gcgcacgtgc ggggtggcgg agagatggga aagaagtggc atgaccaggg
     2581 gaagatgttc gcgaagaaga acacgttcac cgacttcatc tcggtggccg agtacctcgt
     2641 ccacgagaag tggacgtcac ccgatcgtct cgtgatcgag ggcgggagcg ccggcggcct
     2701 cctcgtgggc g
//
LOCUS       DASWAV010000201         2267 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U2665
            k141_91515, whole genome shotgun sequence.
ACCESSION   DASWAV010000201 DASWAV010000000
VERSION     DASWAV010000201.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960316
            Sequence Read Archive: SRR10260007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2267)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2267)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:47:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,929
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2267
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_91515"
                     /isolate="SMAG_U2665"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.36444 N 132.31889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(161..988)
                     /gene="proC"
                     /locus_tag="VF958_04010"
     CDS             complement(161..988)
                     /gene="proC"
                     /locus_tag="VF958_04010"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013007997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004735 - pyrroline-5-carboxylate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HEX9148306.1"
                     /translation="MPARKIGVIGVGNMGTALLRGILASEWGRKTNLLASHPKKAKAA
                     ALARELGIRVIGQNLEVARHADILILGVKPQILGPVLTEIRPALRERQLLISIAAGFP
                     THQIESILGAPLPVIRAMPNVAAVVRLSATVLCLGRHAKSAHAAEARRIFESIGTVVE
                     LPEYQLDAVTGLSGSGPMYIFQIIEGLSDAGVRVGLSRDVSTALTIQTVLGAAKMAEV
                     LKTHPGILKDLVTSPGGTAIAALHSLERNRLRAILMDAVEVATARSKELGQSSSGKT"
     gene            1064..>2267
                     /locus_tag="VF958_04015"
     CDS             1064..>2267
                     /locus_tag="VF958_04015"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEX9148307.1"
                     /translation="MTLAAGTRLGPYEILAPLGAGGMGEVYRARDAKLDRDVAIKVLP
                     ESVAADPDTMARFEREAKAVAALTHPNILAIHDFGTHEGISYAVMELLEGQTLRERLL
                     AGPIPPKQAIDFALQVAKGLSAAHDKGVVHRDLKPENLFVTRGGHLKILDFGLAKRVP
                     AVTPGAETNAPTRSDQTVPGTVLGTLGYMSPEQVRGLPVDHRSDIFSLGAVLHELLSG
                     RKAFNRTTPSDTIAAILREEPPALTGGDIPVALDLVVRHCLEKDRADRFQSARDVTFA
                     LSQASSSSVTTVPAVAARPTKSLLVPVAAALLLAAAAIALLVLRRTPAAPVGPPTVRR
                     LAVLPFENLGSPEDDYFADGIADEIRAKLTSLSGVEVIARGSSTPYKKTTKSQTKIAG
                     ELGVGYLLT"
BASE COUNT          370 a          772 c          740 g          385 t
ORIGIN      
        1 cgaggcgcag cttcggtccc agaggtctcg aggagacgac cgtcgacgcg ctcgtccgcg
       61 cggtcggcaa cgaggtctcc gagacctcga ccgacgcgga ctgatcgacg cctcgcggtt
      121 ggctctcgcc aaccccgacg atcggtcgcg tcgcgacact tcaggtcttg ccggagctcg
      181 actgcccgag ctctttcgaa cgggcggtgg cgacttcgac ggcatccatc agaatcgccc
      241 ggagccggtt gcgttccagg ctgtggagcg cggcgatcgc ggttccgccc ggggacgtga
      301 cgagatcctt caggatcccc ggatgagtct tcaggacttc cgccatcttg gcggctccga
      361 ggacggtctg gatggtgaga gccgtgctga cgtcgcggga aaggccgacg cggacccccg
      421 cgtcgctcaa accctcgatg atctggaaga tgtacatggg gccgctgccg gagagcccgg
      481 tgacggcgtc gagctgatac tccgggagtt cgacaacggt gccgatcgat tcgaagattc
      541 tccgagcctc cgcggcatgc gcggacttcg cgtgacgccc gagacagaga accgtcgcgc
      601 tcagccggac gacggcggcg acgttgggca tggctcggat gacgggcagc ggcgcgccga
      661 ggatcgattc gatctggtgt gtcgggaagc cggcggcgat cgagatcagc agctggcgct
      721 cgcgcagggc cggccggatc tcggtcagaa ccggcccgag aatttgcggc ttgacgccga
      781 ggatcaggat atccgcgtga cgcgccactt ccaggttctg cccgatgacc cggattccga
      841 gctcgcgcgc gagcgccgcg gccttcgcct ttttgggatg cgaggcgagg aggttcgtct
      901 tcctgcccca ctccgaggcg aggattcccc gcaacagggc cgtccccatg tttcccacgc
      961 cgatgacgcc aatttttctg gccggcattt ccacagtcta ctttcgcctg ctggcggggc
     1021 gctgccgctg tcgcctcgat agaatcgccg ccgcagaaaa gcaatgacac tcgcggccgg
     1081 cacgcgtctc ggcccttacg agattctcgc gcctctgggc gcgggcggaa tgggcgaggt
     1141 ttatcgggcg cgagacgcga agctcgatcg cgatgtcgcg atcaaggtac ttcccgagtc
     1201 ggttgccgcc gatccggaca ccatggcgcg gttcgagcgc gaggcgaagg cggtcgcggc
     1261 gctcacccac ccgaacatcc tcgcgatcca cgatttcggc acgcacgaag gcatctctta
     1321 cgcggtcatg gagcttctgg agggtcagac gcttcgcgag aggctcctcg cgggcccgat
     1381 tcctccgaag caggccatcg acttcgcgct gcaggtcgcc aaaggtctct cagccgctca
     1441 cgacaagggc gtggtccacc gggacctcaa gcccgagaac ctcttcgtca cccggggtgg
     1501 ccacttgaaa atcctcgact tcggcctcgc gaagagagtc ccggcggtga cgccgggagc
     1561 agagacgaac gcacccactc ggtcggacca aacggtgccc gggacggtgc tgggaacatt
     1621 gggatacatg tcgcccgagc aggttcgcgg acttcccgtc gaccatcgat cggacatctt
     1681 ctctctcggc gcggtcctcc atgagctgct ctccggcagg aaagctttca acaggaccac
     1741 gccgagcgac acgatcgccg cgatcctgag ggaggagccg ccggcgttga ccggcggaga
     1801 catcccggtc gcgctcgatc tcgtcgtgcg ccactgtctg gagaaggacc gcgccgaccg
     1861 tttccaatcg gcccgggacg tcacgttcgc actctcgcag gcttcgtcgt cgtccgtgac
     1921 gaccgttccc gcggtggccg cccgaccaac caaatcgctt ctcgtgcccg tcgcggccgc
     1981 gttgctcctc gcggccgccg ccatcgcgct cctggtcttg cggcgcactc cggcagcccc
     2041 ggtcgggcct cccaccgtcc ggcgcctcgc cgtgctgcct ttcgaaaacc tcggatctcc
     2101 ggaggacgac tacttcgccg acggcatcgc tgacgagatt cgggccaagc tcacgtccct
     2161 ctccggtgtg gaagtgatcg cgcgcgggag ctccaccccg tacaagaaga cgacgaagtc
     2221 gcagacgaag atcgccggcg agctcggtgt cggctacctc ctcacgg
//
LOCUS       DASWAV010000202        11609 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U2665
            k141_411905, whole genome shotgun sequence.
ACCESSION   DASWAV010000202 DASWAV010000000
VERSION     DASWAV010000202.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960316
            Sequence Read Archive: SRR10260007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 11609)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11609)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:47:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,929
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11609
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_411905"
                     /isolate="SMAG_U2665"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.36444 N 132.31889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1209)
                     /locus_tag="VF958_04020"
     CDS             complement(<1..1209)
                     /locus_tag="VF958_04020"
                     /inference="COORDINATES: protein motif:HMM:NF012602.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HEX9148308.1"
                     /translation="MNFNRRRFLGFAAGAVAGTAVGVPAGRTIGDFLQASELPVYPPR
                     GPEKFVLSVCNLCPGGCGLRVRRIGERAVKVDGNPLHPVNGGRLCPRGQAALQGLYHP
                     DRFTGPLRRVGKRGSLASFQKVPWNRALEEVAGRLRQIRESGHPESLVLLRGGARRVG
                     SRLAQRYVAAFGSPNDVTLERGEVSSSLAMQLGQGVLAPPAYEIGSADYVLSLGGALL
                     EAWSSPVHTARAYGRFRQGRTGQRGKFVQVEPRLSITAASADEWLTVRPGTEGVFGLG
                     VAAVILAEGLYDREFVLERVSGLEDGNGENGEPRRGLRTLLEREYSLERVAEETGVAA
                     NVILRVAREFAAAHRALAVGPRRGPLLPGSLYGHLAAQTLNALSGSIDAPGGVLLPED
                     VPLSPWPPLAE"
     gene            complement(1241..1753)
                     /locus_tag="VF958_04025"
     CDS             complement(1241..1753)
                     /locus_tag="VF958_04025"
                     /inference="COORDINATES: protein motif:HMM:NF014176.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c3 family protein"
                     /protein_id="HEX9148309.1"
                     /translation="MTGARGWLLAGVSGFLLFLIGSAVPSAVRRPSARQPIAFNHAKH
                     VKDNDLACSTCHEYYEKQAFSGLPTSEVCASCHLEPQGKSEEERKLVRLLKAGAPLEW
                     KPLFLQPPHVYYSHRRHVMTAKIDCSVCHGGIAQTSFPPTRVRKLRMADCIGCHEKRG
                     APTQCTACHR"
     gene            complement(1750..2994)
                     /locus_tag="VF958_04030"
     CDS             complement(1750..2994)
                     /locus_tag="VF958_04030"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HEX9148310.1"
                     /translation="MQTSASGPRSDSPRRRSFFFWAVRIAAAVGAVLLLVAVLKWEEK
                     RSTNRWSTLVEGSPRTGEKLFEAKGCSSCHTGAEAAPGPNLSVETATRSGPDHLVTAM
                     WNHAPKMWERMHEAGVVPPAFTQQEMANLLAYLYTLRYVGAAGDEVRGERLFVGKGCA
                     SCHNVRGKGLHGAKDLSSVGSAATAVGFATAMWNHPKNRGESEGSRFDGVDANDVLSY
                     MRGGGVARQLDSQLVAADFQRGWTVFREKSCTACHSVKDEAGHLGPELGPGRDLPPTV
                     VQLAGAIWNHSPAMWDAMEHLRIQRPTFREREMADLVAFLYSFRYAEPGGSPKVGEVL
                     FAARGCSRCHGPLALGTREGPELRGRGKSFTSVTMAAALWRHGPAMFRRTRDLGLPWP
                     VLAENDVGDLITFLNTSSEPTR"
     gene            complement(3216..4601)
                     /locus_tag="VF958_04035"
     CDS             complement(3216..4601)
                     /locus_tag="VF958_04035"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HEX9148311.1"
                     /translation="MSPVPTPHPYGILVVDDDERLAATLQELLVREGYAVEVALSAAE
                     AVTILERRRGIGVALVDLIMPGSDGIALMDELHRRDPELAVIVMTGYGTIETAVEAIK
                     RGAEDYLTKPMDSHALRKKMARMMEVFELRERVRQLETNLESAPSFEGIISVSPSMRR
                     VIERAQTVAATESSVLLLGETGTGKEMMARAIHATSPRAGRVFLPVNCGALPRELVES
                     ELFGVRRGAYTGASADTPGIFAAATGGTVFLDEIGEMPREAQVKLLRVLQERELRPVG
                     SATTVHVDVRIIAASNRPLPTLRGEQLRPDFYFRIATVVLEIPPLRARPEDVLVLAQH
                     FLGRLSRRSRREIVLAHSAADLLVAYPFPGNVRELENVLESATAVSRDDPQTLTDRDL
                     KPLLGDAASFPMTDGVPRESLSLERLEDIAIQQALHVCGGNRTRAAALLGISRDTLYR
                     KLRQARERENS"
     gene            complement(4598..5857)
                     /locus_tag="VF958_04040"
     CDS             complement(4598..5857)
                     /locus_tag="VF958_04040"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX9148312.1"
                     /translation="MSIVGIVFAAWELVESRFFRDVDYTTLHYLYISRGIASSLLLAA
                     WAAWYVLRVRRKSEEELRRSWEHYRRLLEASPGAVALLDSTLRVTEWNATAERLYGFA
                     REEVLGGRLPTVPQDRSDELRHLLDEVRAGRPVNDLETRRCERGGQSFEVQLTLLPFR
                     DSAGQDLFLEVTSDIRERVRMRERLLEIEKLASMGKMAAGTAHHLNTPLAAMLLRVQM
                     MRERTQHALGADLERLENDIGYCQQFVQRLLEFGRRPYAEKEPQPIASCLSSVASFLA
                     PAMVPRSVRLALDLDTADGEQVLADRNLLEALFLILLSNALDAVPDGGGIAVRCNRPG
                     KDRIEIQIADEGCGIAPSHLPHVFEPFYTTKGPGKGTGLGLAIARNIVIEHGGSIRIE
                     SSPGQGTIAAVELPLWRRNASGETPTQ"
     gene            6244..6564
                     /locus_tag="VF958_04045"
     CDS             6244..6564
                     /locus_tag="VF958_04045"
                     /inference="COORDINATES: protein motif:HMM:TIGR01764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX9148313.1"
                     /translation="MKNRNGSRSRSEARGGERNGSPGRSDSRGGNRNGSSSSPDAPAG
                     SRFLSRGEVSQLFGVSPSTVTRWARLGLLKAARTPGGHYRFPAEETLKAASRAAAGEL
                     SRLD"
     gene            6569..8494
                     /locus_tag="VF958_04050"
     CDS             6569..8494
                     /locus_tag="VF958_04050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012632832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEX9148314.1"
                     /translation="MALALRFPDLAHWKAQVKCQMGCPVATDAGRYVQLIAEGRDEDA
                     FLVARAPNPFASVCGRVCAAPCEDACRRGSIDGPISIRALKRYVTERYGVESVHPDTQ
                     DRLLSELISEGNRYPGHLPVHPLPGAPEPEWRKVAVVGAGPAGLSAAADLALLGYAVT
                     VFEAAEEPGGMMRFGIPEYRLPRTLLAAEIDKIVQLGVNLETRRPLTADFGLGKLRAL
                     GFEAVFLSVGVSKGRDLAVPGVEKDGVVKAVDYLLNVNRGYRLNLGRRVIVIGGGFVA
                     FDAARTALRLATPEAEAELAAAADARVKEALDSARSAMRGGAAEVTIVSLENFEEMPV
                     LRTTQGREEFEEAKREGIRFVTRRGPKELLGGARLEAVALRRVVSVFDSNGRFAPTYD
                     DSDVETFPADACVLAIGQRADLDFLDPSDGVVLTAAGGIRVDPVTLATSAPGIFAGGD
                     VAFGPRNLIEAVANGKRAARSIHEYLSREAAEIETELEIEKIPTSRYRMIAGFEILDR
                     EAAPMLDLDRRTGISEVETGYGMDEARRQAARCLVCHVQTIYDPEKCVLCSRCVDVCP
                     EYCLALVPIEELDIPEDERRLLAERAEASGGVPLSVMIKDDERCIRCGLCAIRCPTDA
                     MTMERFSITQRFATAAS"
     gene            8506..9012
                     /locus_tag="VF958_04055"
     CDS             8506..9012
                     /locus_tag="VF958_04055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011421457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske 2Fe-2S domain-containing protein"
                     /protein_id="HEX9148315.1"
                     /translation="MTEKANRRDFLLKLGIGSGIAALGAQTVASMRSLVPNVSYDAPT
                     TVKLGAPNDFPDGLKFLPDERLFVFRQGNTFHAVSAVCTHLGCTVRAEALSQPELKTV
                     GGQSLKLTHRFLCPCHGSRYSGDGSNVSGPAPKPLAWYHLSVALDDGQLVVDLARPVE
                     HDFRLTIA"
     gene            9023..10384
                     /locus_tag="VF958_04060"
     CDS             9023..10384
                     /locus_tag="VF958_04060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011984566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b N-terminal domain-containing
                     protein"
                     /protein_id="HEX9148316.1"
                     /translation="MSTDLRELEATFSGAAPEAPGFRSRRFWSWRPASDRQAGDAVVS
                     NFLLHWFPAKSYRASLAWSYSFWLGTVSAALLLLLVVSGLPLLFLYIPSVERAYASVK
                     DIEYVVTFGSWIRAVHRISAHLMVVAVALHLVRVFLTGAYKNALGGGQRRQWNWLLGV
                     AMLLLTLFLSFTGYLLPWDQLAYWAVTVGTNIASSVPALGPTIRELLIGGRTIEQPTL
                     IRFYVLHVLFLPGLLGVLFAYHMWRVRKDGGLASADRAALATRRVETPPSKTKTYTLL
                     GVARGTAPRIRAATVESGEATVNSVPDLARRAAIVVLGTIALIGVLSVFVRSPLEEPA
                     NPLITPNPAKAPWYFLWLQEVVTDTTLHIGRITINGAFLGGVILPGLLVGLLTVWPWL
                     DRSPSAAAGAWFPSSRRRQVAVFLAILAAVVLLTIVGTFLRGPYWHFYWPWQPWPQLP
                     SRI"
     gene            10386..>11609
                     /locus_tag="VF958_04065"
     CDS             10386..>11609
                     /locus_tag="VF958_04065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012632835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HEX9148317.1"
                     /translation="MEKRSDSPFPKKDRPVLAAVGVLLVISTVLFAWSDRRHDWRWYQ
                     YDFRRQITEKFGAEKASGIPKGVQQIWSAELRRADRCITCHQATNWKGFETAKNPYRT
                     HPARILRNHPIEKYGCTSCHGGQGWAIDVDDAHAPAEHWEEPLLGSSLGEAYSLVDDK
                     KALMQMNCNVCHRYDRETPGADSINLAKALVREKGCRACHVINGRGGTIGPDLTNVGD
                     KAPEQYEYGRLSGQKSAFAWHVAHFKDPRALVPETVMPNFHLSTKEAQSLAMLALSWR
                     RAPVPADYRPGVPRTDPPTPEETAEEERMKTGPGAWFVKTGCFVCHSVRALGVKSPAQ
                     IGPDLSTAVEDVQSRFGVTVDDFLRAPTGTMAVVLSRQIVLSPEEKAVAVQKLREAFA
                     EHQKQKAAAPQERPAG"
BASE COUNT         1860 a         4032 c         3897 g         1820 t
ORIGIN      
        1 ctcggcaagc ggaggccagg gtgagagcgg tacgtcctcg ggcaatagca ctccgccggg
       61 cgcgtcgatc gagccagaga gagcgttcaa cgtctgcgcc gcgagatgac cgtagaggga
      121 cccgggaagg agcggcccgc gccgcggacc gacggcgagc gcccggtgcg cggcggcgaa
      181 ttcccgagcc acccgaagga tgacgttcgc ggcgaccccg gtctcttcgg ccacgcgctc
      241 caggctgtac tcgcgctcga gaagcgtgcg gaggccgcgc cgcggctcgc cgttctcccc
      301 gttcccgtcc tcgaggcctg acacccgctc caggacgaat tcccggtcgt agagcccctc
      361 cgcgagaatc acggccgcga ccccgaggcc gaacactcct tccgttcccg gccgcacggt
      421 gagccactcg tccgcggagg cggctgtgat cgagagacgc ggctcgacct gcacgaactt
      481 cccgcgctgc ccggtcctcc cctggcggaa gcggccatac gcccgggcgg tgtggaccgg
      541 agagctccac gcctcgagca gagcgccgcc cagcgagagc acgtagtccg ccgacccgat
      601 ttcgtaggca ggcggggcga gaacgccctg gccgagctgc atcgcgagcg acgaggagac
      661 ctcgccccgc tcgagcgtca cgtcgttcgg agagccgaac gcggcgacgt accgttgcgc
      721 aagccggcta ccaacccggc gcgctccgcc gcgcaggagg acgagggatt cgggatgtcc
      781 gctctcgcgg atctgccgga gacgtccggc gacctcctcg agcgctcggt tccacggaac
      841 tttctggaag gaggcgagcg agccgcgctt cccgacgcgg cggagcggcc cggtgaagcg
      901 atccgggtga tagaggccct gcagtgccgc ctgcccgcgg gggcacagac gtcctccgtt
      961 gaccggatga agcggattcc cgtcgacctt gactgcccgc tcaccgatgc gccggacgcg
     1021 gagcccgcag ccgcccgggc agaggttgca gacggaaagg acgaacttct cgggacctcg
     1081 tggcgggtag accgggagct cggacgcctg caggaaatcg ccgatcgttc ggcccgccgg
     1141 aacgccgacc gcagtgcccg ccaccgcgcc cgccgcgaag ccgaggaacc gcctgcggtt
     1201 gaaattcacg gctcgacccc tcgcgacgtg agcgtccggg tcatcggtga catgccgtgc
     1261 actgcgtcgg cgccccgcgc ttctcgtggc agccgatgca gtccgccatc cgcaacttcc
     1321 ggacccgggt cggaggaaaa gaggtctgcg cgatcccccc gtggcagacc gagcagtcga
     1381 tctttgcggt catcacgtgg cggcgatgcg agtagtagac gtgcggaggc tgcaagaaga
     1441 ggggcttcca ctcgagcgga gctcccgctt tcaagagccg gacgagcttg cgctcttcct
     1501 cgctcttccc ctgcggctcg aggtggcagg aagcgcagac ctcgctcgtc ggaaggcccg
     1561 agaacgcttg cttctcgtag tactcgtgac aggtcgagca ggcgaggtcg ttgtccttca
     1621 cgtgcttggc gtggttgaag gcgatcggct gccgcgccga cggccggcgc accgcggacg
     1681 gcacagcgct cccgatcagg aaaaggagaa agccggaaac gcccgccagg agccagcccc
     1741 gcgcaccggt caccgcgtcg gctcggagga ggtgttcaag aacgtaatga gatccccgac
     1801 gtcgttctcc gcgaggacgg gccacggcaa accgagatca cgcgtccggc ggaacatcgc
     1861 ggggccgtgg cgccagagcg cggccgccat cgtcaccgaa gtgaagctct ttccccgccc
     1921 gcgaagctcg ggcccttcgc gcgtccccag cgccagcggg ccgtggcagc ggctgcagcc
     1981 gcgggccgcg aagaggacct cgccgacttt gggggagccg cccggctcgg cataacggaa
     2041 gctatagagg aacgcgacca ggtcggccat ctcccgctcg cggaacgtcg gacgctggat
     2101 ccgcaggtgt tccatcgcgt cccacatcgc gggcgagtgg ttccagatcg ccccggcgag
     2161 ctggacgacc gtgggtggca gatcgcggcc gggaccgagc tccggcccga ggtggccggc
     2221 ttcatccttc accgagtgac aggcggtgca cgatttctcg cgaaacacgg tccagccgcg
     2281 ctggaaatcc gccgcaacga gctgagagtc gagctgccgg gcgacgccgc cgccccgcat
     2341 gtagctgagg acgtcgttcg catcgacccc gtcgaatcgg ctaccttcgc tctcaccgcg
     2401 gttcttcggg tggttccaca tcgcggtcgc gaacccgacc gcggtcgccg ccgagccgac
     2461 gctcgagaga tccttcgcgc cgtgcaaccc cttgccccgc acgttgtggc agctcgcgca
     2521 gcccttgccc acgaacagtc gctcgccccg gacctcgtcg cccgcagcgc cgacgtagcg
     2581 aagcgtgtag aggtacgcga gcaggttcgc catctcctgt tgtgtgaacg cgggcgggac
     2641 gactcccgcc tcgtgcatgc gttcccacat cttcggagcg tggttccaca tggccgtgac
     2701 gaggtgatcg gggcccgagc gcgtcgcggt ctcgacggac aggttcggac cgggcgcggc
     2761 ctcggcgccc gtatggcagg aagaacatcc tttcgcctcg aagagcttct cgccggtcct
     2821 cgggctgcct tcgacgagcg tcgaccatcg attcgtcgat cgcttctctt cccacttcag
     2881 gacggcaacg agcaggagga ccgcgccgac cgcggccgcg atccgcaccg cccagaagaa
     2941 gaacgagcgg cgtcgcggac tatccgatcg cgggcccgaa gcgctggttt gcaagtgtct
     3001 cttcgctcgt ggaagagcac acgccctgcc atcattcatc ggatgccggc cgcgcgtcgg
     3061 gggacgccgg agtcttcgga tcgatcgaat ccgatcaacg agttacgcag cgagacgagc
     3121 gcgtcgaacc ggcggggctc gtgcgccccg gctaccgccg aatgtcgcag aatccaacgc
     3181 cgtccggaat tcggacgcgc ggtggtcggg tcggttcagc tgttctcgcg ctcacgcgcc
     3241 tgccgcagct tgcggtagag cgtgtcgcgc gagatgccga ggagcgcggc ggcgcgggtg
     3301 cggttgccgc cgcagacgtg cagcgcctgc tggatcgcga tgtcctcgag ccgctcgaga
     3361 gacagggatt cgcggggaac gccgtcggtc atcgggaagg aagccgcgtc tcccaacagc
     3421 ggtttcaggt cgcggtccgt aagcgtttga ggatcgtctc gggaaacggc cgtggcggac
     3481 tcgaggacgt tttcgagctc gcgcacgttg cccggaaacg gataggcgac gagcaggtcc
     3541 gccgcggaat gcgcgagcac gatttcacgt cgcgagcgcc gcgagagtct tccgaggaag
     3601 tgttgggcca gcacgagcac gtcctcaggg cgcgcgcgca ggggggggat ctcgagcacg
     3661 acggtcgcga ttcgaaaata gaaatcgggt cggagctgct ccccccgcaa cgtcggaagc
     3721 ggccgattgg acgccgcgat gatccggacg tcgacgtgca cggtcgtcgc gctgccgacc
     3781 ggacgcagct ctctctcctg gagaacccgc aggagcttga cctgcgcctc gcgcggcatt
     3841 tcgccgatct cgtcgagaaa aacggttcct ccggtcgcgg ccgcgaagat gccaggcgtg
     3901 tccgcggagg cccccgtgta ggcgccgcgg cggacgccga agagctcgct ctccacgagc
     3961 tctcgcggca acgcgccgca gttgaccgga aggaagacgc ggccggcccg gggactggtg
     4021 gcgtggatcg cgcgcgccat catctctttt ccagtgccgg tctcgccgag aagcaggacc
     4081 gacgactccg tggcggccac ggtctgcgcg cgctcgatca cgcggcgcat cgaaggcgat
     4141 acggagatga tcccctcgaa agaaggagcg ctttcaaggt tcgtctcgag ctggcggaca
     4201 cgctctcgca gctcgaagac ctccatcatc cgggccatct tcttgcgaag agcgtgcgag
     4261 tccataggct tggtcagata gtcttcggcg cctcgcttga tcgcctcaac cgccgtctcg
     4321 atcgtgccgt atccggtcat gacgatcacg gcgagctccg gatcccggcg gtgaagctcg
     4381 tccattagag cgatgccgtc ggagcccggc atgatcaggt ccaccagcgc gacgccgatt
     4441 ccgcgccggc gctcgagaat ggtcacggcc tccgcggccg acagcgccac ctcgacggcg
     4501 tacccttccc gcacgagaag ctcctgcagc gtcgcggcga gccgctcgtc gtcgtccacg
     4561 acgagaatcc cgtaggggtg cggagtcggg accgggctca ctgcgtgggc gtctccccag
     4621 acgcgttccg gcgccagaga gggagctcga cggcggctat ggttccctgt cccgggctgc
     4681 tctcgatacg gatcgatccg ccgtgctcga tcacgatgtt gcgcgcgatg gccaggccga
     4741 gccccgtccc ctttccggga cctttcgtcg tgtaaaaggg ctcgaacacg tgcgggaggt
     4801 gcgagggcgc gattccgcac ccctcgtcgg cgatctgaat ctcgatcctg tccttgcctg
     4861 gacggttgca tcggacggcg attccgccgc cgtcggggac ggcgtcgaga gcgttggaga
     4921 gaaggatcag aaagagggcc tcgaggagat tccgatccgc cagtacctgc tccccgtcgg
     4981 ccgtatcgag atcgagagcg agacggacgc tacggggcac catcgctggc gcgagaaagc
     5041 tggccaccga ggagaggcag ctcgcaatcg gctgcggctc cttctcggcg tacgggcggc
     5101 gaccgaactc gaggagccgc tggacgaact gttggcagta gccgatgtcg ttctcgagcc
     5161 gctcgaggtc ggcgccgagg gcgtgttgag ttctttcacg catcatctgc acacgcagca
     5221 gcatcgcggc gagcggcgtg ttcaggtggt gcgccgttcc ggctgccatc ttccccatgc
     5281 tcgcgagctt ctcgatctcg agaagacgct cgcgcatgcg cacgcgctcc cggatatcgg
     5341 acgtcacttc gagaaacagg tcctgacccg ccgaatcccg gaaaggcagc agggtcagct
     5401 gaacctcgaa gctctggccg ccgcgttcgc agcggcgggt ctcgaggtca ttgacgggcc
     5461 gacccgcgcg aacctcgtcc agcaggtgac gcagctcgtc gctccgatcc tgcggcacgg
     5521 tgggcagacg accgccgagg acttcttctc tcgcgaagcc gtagagccgc tcggccgtgg
     5581 cgttccactc cgtgacgcgc aacgtcgaat cgagaagtgc aacggcgccg ggcgaagcct
     5641 ccaggaggcg ccggtaatgc tcccaggacc gtcggagctc ttcctcgctc ttccggcgga
     5701 cccgcaggac gtaccaggcc gcccacgccg cgagcaagag agaggatgcg atcccacggc
     5761 tgatgtagag gtagtggaga gtcgtgtagt ccacgtcccg gaagaagcga ctctcgacca
     5821 gctcccacgc agcgaagacg ataccgacga tgctcacgca ggacaggagt agagtcaccc
     5881 agaaccagcc agcctcttcg acgcggccct tcgcgaggcg actgcccgga acgtctcggg
     5941 actcttcgga gctcgtcgtc agcctctccc ggctctgggc acgtcggcgt gccgcggtgc
     6001 ggaaaacttc ggctgatcgt aacgcaaggc cgctccgcgg ctcgcccaga cctgccgatc
     6061 gaccgccgcg cacttttgat ccacctgcat cattccctcc acccgggaac cgtgcaagct
     6121 ccgcctggag gcgggagaac ggcctaaagg gatcgaacga gtaaaaaaga gcgaaaagac
     6181 cggcgctatg acgcgcgtca tgtgccgctc gtgcgattca gctgaggctg ttcccggcga
     6241 cagatgaaaa atcggaacgg ttcgcggagt cggtcggagg cacgcggcgg cgagcgcaac
     6301 ggttccccgg gtcgctcgga ctcgcgcggc ggcaatcgca acggttcctc cagctctccg
     6361 gacgcccccg ccggctcccg atttctttcg cggggtgagg tgtcgcagct cttcggagtc
     6421 tcgccttcga cggtcacacg ctgggcccgg ctcggtctct tgaaggccgc gcgcaccccg
     6481 ggcggccact accgctttcc cgccgaagag accttgaaag ccgcgagccg cgctgcggcg
     6541 ggcgagctct cgcggctcga ctgacacgat ggctctcgcg ctccgctttc cggatctcgc
     6601 ccactggaag gcccaggtca agtgccagat gggctgtccc gtggcgaccg atgcggggcg
     6661 ctacgtccag ttgatcgccg aggggcgcga cgaggacgcc tttctcgtcg ctcgcgcgcc
     6721 gaatccgttc gcgtccgtct gcggccgcgt ctgcgccgcc ccgtgcgaag acgcttgccg
     6781 acgtggatcg atcgacggtc ccatctcgat tcgcgcgctc aagcgttacg tgacggaacg
     6841 ctacggcgtg gaatccgtgc accccgacac gcaggaccgg ctcctctcgg agctcatctc
     6901 cgaagggaac cgatatccgg gtcatctgcc cgtgcacccg ctccccggcg cgcccgaacc
     6961 cgagtggcga aaggtcgccg tggtcggcgc gggaccggcg ggcctttccg cggccgccga
     7021 cctggctctg ctcgggtacg ccgtcaccgt atttgaggcg gccgaggagc ccggcggaat
     7081 gatgcgcttc gggatccccg agtaccgtct gccccggact ctcctcgcgg ccgagatcga
     7141 caagatcgtc caactcgggg tgaacctcga gacccgccga cctctcaccg cggacttcgg
     7201 cctcggcaag ctccgagcgc tcggcttcga ggcggtgttc ctgtcggtgg gcgtctcgaa
     7261 gggccgcgac ctcgcggtgc cgggcgtcga gaaagacggc gtcgtcaagg ccgtggacta
     7321 tctcctgaac gtgaaccgcg gctatcgcct gaatctcggc cgccgggtca tcgtgatcgg
     7381 cgggggcttc gtcgcgttcg acgcggcgcg tacggcgctt cgtctcgcga ccccggaagc
     7441 cgaggcggag ctcgccgccg cggccgacgc acgcgtcaaa gaggcgctcg actcggcccg
     7501 ctccgccatg cgcggcggcg cggccgaagt gacgatcgtg tcgctcgaaa acttcgagga
     7561 aatgccggtg ctgcgcacga cgcagggccg cgaggagttc gaggaggcga aacgcgaggg
     7621 cattcgattc gtcacccgcc gcggcccgaa ggagcttctg ggcggagccc gcctcgaggc
     7681 cgttgcgctg cgacgggtcg tctccgtctt cgactcgaac ggccgcttcg cccctacgta
     7741 cgacgactcg gacgtcgaga cgttcccagc ggacgcctgc gtccttgcga tcggccagcg
     7801 cgcggacctc gactttctcg acccctccga cggcgtcgtg ctcaccgccg ccggtgggat
     7861 ccgcgtcgac cccgtcaccc tcgcgacatc ggcaccggga atcttcgccg gaggcgacgt
     7921 cgcgttcggc ccccgcaacc tgatcgaggc ggtcgcgaat gggaagcgcg cggcgcgctc
     7981 gatccacgag tacctgtcgc gcgaggcggc ggagatcgag accgagctcg agatcgagaa
     8041 gatcccgacg tcgcggtacc ggatgatcgc ggggttcgag atcctcgacc gcgaggcggc
     8101 tccaatgctc gatctcgacc ggaggaccgg gatctccgag gtcgagaccg gctacggaat
     8161 ggacgaggcg cggcggcagg cggcgcggtg cctcgtctgc cacgtccaga cgatctacga
     8221 cccggagaag tgcgtcctct gcagccgctg cgtggacgtc tgtcccgaat actgcctcgc
     8281 gctcgtgccg atcgaagaac tcgacatccc ggaggacgag agacgcctgc tcgccgaacg
     8341 cgccgaagcg tccggcggcg ttcccctctc cgtgatgatc aaggacgacg agcgctgtat
     8401 ccggtgcggg ctctgcgcga tccgctgtcc caccgacgcg atgacgatgg agaggttctc
     8461 gatcacgcag cgcttcgcga ccgcggcttc gtgaaggagc aagtcatgac agaaaaggca
     8521 aaccgccggg actttctgtt gaagctcggg atcggcagcg ggatcgcggc gctcggcgcg
     8581 cagaccgtcg cgtccatgcg ctcgctcgtg ccgaacgtct cgtacgacgc accgacgacc
     8641 gtcaagctcg gcgcaccgaa cgatttcccg gacggactga aattccttcc cgacgagagg
     8701 ctcttcgtct tccggcaggg gaacacgttc cacgccgtct ccgcggtgtg cacccacctg
     8761 ggatgcaccg tccgcgccga agcgctctcc cagccggagt tgaagaccgt cggcggccag
     8821 tcgctcaagc tgacgcaccg cttcctctgc ccctgccacg gctcccggta ctcgggggac
     8881 ggatcgaacg tttcgggtcc ggcgccgaag ccgctcgcct ggtaccacct ctccgtcgcg
     8941 ctcgacgacg gacagctcgt cgtggacctc gcgcgccctg tggagcacga cttccgcctg
     9001 acgatcgcgt gaaggagacc gcatgagcac cgacctccgc gaactcgaag cgactttctc
     9061 cggcgccgca ccggaggcgc cgggcttccg ctcccggcgg ttctggagct ggcgcccggc
     9121 ctccgaccgc caggccggag acgcggtcgt ttcgaacttc cttctccact ggttcccggc
     9181 caagagctac cgggcgtcgc tcgcctggag ctactcgttc tggctcggaa cggtgtccgc
     9241 ggcgctgctc ctcctcctcg tggtctccgg tctgccgctc ctcttcctct acatcccctc
     9301 ggtcgagcgc gcgtacgcgt cggtcaagga catcgagtac gtcgtgacgt tcggctcctg
     9361 gattcgcgcg gtccaccgca tctcggcgca cctgatggtc gtcgccgtgg ccctccacct
     9421 ggttcgcgtg ttcctgacgg gcgcttacaa gaacgccctg ggaggcgggc agcgccgtca
     9481 gtggaactgg ctgctcggcg tcgcgatgct gctcctgacc ctctttcttt ccttcaccgg
     9541 ctacctgctc ccctgggacc agctcgccta ctgggcggtg acggtcggca cgaacatcgc
     9601 gtcgtcggtt cccgctctgg gcccgacgat ccgcgagctc ttgatcggcg ggaggacgat
     9661 cgagcagccg acgctgattc gcttctacgt gctccacgtg ctgttcctcc cgggcctgct
     9721 gggggtcctc ttcgcgtacc acatgtggcg ggtgcgcaag gacggcgggt tggcctccgc
     9781 cgaccgcgcc gcactggcga cgcgacgggt cgagacgccc ccctcgaaga cgaagaccta
     9841 cacgctgctc ggcgtggcgc ggggaaccgc cccgaggatc cgcgccgcga cggtcgagtc
     9901 gggcgaggcg acggtgaact cggtcccgga tctcgcgcgc cgcgccgcga tcgtcgtgct
     9961 cggcacgatc gccctgatcg gcgtcctctc ggtcttcgtc cgctccccgc tcgaagagcc
    10021 ggcgaatccg ctcatcacgc cgaaccccgc gaaggcgccc tggtacttcc tgtggctcca
    10081 ggaggtcgtc accgacacca ctctccacat cgggaggatc acgatcaacg gcgcgtttct
    10141 cggcggagtg atcctgcccg ggctgctcgt cggtcttctc acggtctggc cgtggctcga
    10201 ccggagccct tcggccgcag caggcgcgtg gttcccctcg agccggcgcc gtcaggtcgc
    10261 ggtcttcctc gccatcctcg ccgccgtcgt gctgctcacg atcgtcggca cgttcctgcg
    10321 cgggccctac tggcacttct actggccgtg gcagccatgg ccgcagctgc cctcgaggat
    10381 ctgacatgga aaagagatcc gactcccctt tccccaagaa ggaccgaccc gtcctcgcgg
    10441 cggtcggagt cctgctcgtg atctcgaccg tcctcttcgc ctggagcgac cggcggcacg
    10501 actggcgctg gtaccagtac gacttccgcc gccagatcac ggagaagttc ggcgccgaga
    10561 aggcgagcgg catcccgaag ggcgtccagc agatctggtc cgcggagctc cggcgcgccg
    10621 accgctgcat cacgtgccac caggcgacga actggaaggg tttcgagacc gcgaagaacc
    10681 cgtaccgtac gcaccccgcg aggatcctga ggaaccaccc gatcgagaag tacggctgca
    10741 cgtcgtgtca cggcggccag gggtgggcga tcgacgtcga tgatgcgcac gcgccggcgg
    10801 agcactggga agagccactg ctcggctcct cgctcggcga ggcgtattcg ctcgtcgacg
    10861 acaagaaggc gctcatgcag atgaactgca acgtctgcca ccgctacgac cgggagacgc
    10921 cgggcgccga ctccatcaac ctcgccaagg cactcgtccg ggagaagggg tgccgcgcct
    10981 gccacgtgat caacggccgc ggcggcacga tcgggcccga cctgacgaac gtcggcgaca
    11041 aggctcccga acagtacgag tacgggcggc tctccgggca gaagagcgcg ttcgcgtggc
    11101 acgtcgcgca cttcaaggat ccccgcgcgc tcgttcccga gaccgttatg ccgaacttcc
    11161 acctctcgac caaggaagcc cagtcgctcg cgatgctggc cctctcgtgg cgccgggcgc
    11221 ccgtgcccgc ggattaccgg cccggggtcc cgcggaccga cccacccaca cccgaggaaa
    11281 cggccgaaga ggagcgcatg aagaccggtc cgggcgcgtg gttcgtcaag accgggtgct
    11341 tcgtctgtca ctccgtgcgg gctctcgggg tgaagagccc ggcgcagatc gggccggatc
    11401 tctcgacggc cgtcgaggac gtgcagtccc ggttcggggt cactgtggac gacttcctgc
    11461 gcgcgccgac cggaaccatg gccgtcgtcc tgtcgaggca gatcgtgctc tcgcccgagg
    11521 agaaagcggt ggcggtccag aagctgcgcg aagcgttcgc cgagcaccag aagcagaagg
    11581 cagccgcgcc gcaggagagg cccgccgga
//
LOCUS       DASWAV010000203         2457 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U2665
            k141_211522, whole genome shotgun sequence.
ACCESSION   DASWAV010000203 DASWAV010000000
VERSION     DASWAV010000203.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960316
            Sequence Read Archive: SRR10260007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2457)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2457)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:47:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,929
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2457
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_211522"
                     /isolate="SMAG_U2665"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.36444 N 132.31889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..339
                     /locus_tag="VF958_04070"
     CDS             <1..339
                     /locus_tag="VF958_04070"
                     /inference="COORDINATES: protein motif:HMM:NF016601.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine/sarcosine/betaine reductase complex
                     selenoprotein A"
                     /protein_id="HEX9148318.1"
                     /translation="LALDSASQEEIKKAAEDHGPENLIVVWGVTNEQFLEVGSRTVME
                     GDPSWSGPLAGEALHLKVFHVLEPEIKNHIPPEVYESELMLLELAIGDEKIGKTIAMM
                     EGFRAQRITG"
     gene            695..>2457
                     /locus_tag="VF958_04075"
     CDS             695..>2457
                     /locus_tag="VF958_04075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019141319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005615 - extracellular space [Evidence
                     IEA];
                     GO_function: GO:0047989 - hydroxybutyrate-dimer hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0019605 - butyrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxybutyrate oligomer hydrolase family
                     protein"
                     /protein_id="HEX9148319.1"
                     /translation="MMEKFKQALPRRTSWAAFALLLGFSFIAVLWAVPALAGEPLNVG
                     MAGTRNEFPDFLVPGSLTAKYYDGGTDDLLTAGLGKTGLLGVEPSVANPQDASQLRRL
                     AIWTNYRALLDIAPAGGYGSLYGPNVENDGTITAGEGKIPGWEYIAVADDGTGRRNVT
                     VMVQIPDRFDAHNACIVTATSSGSRGIYGAISAAGEWGLKQGCAVAYTDKGTGNGLDD
                     LTANLVNRIDGRLADAQVAGKDSLFTANLTDAKRASFNAATPNRFAYKHAHSQQNPER
                     DWGRNTLQAVVFAFHALNDRFGTPAPQGGKRITLDRRNTLVIASGASNGGGAGLAAAE
                     QDQHNLIDGVAVGEPNVNTPADEHLTIRQGATTFPVFGRALIDYFTYANLYQPCAALD
                     PRAATAPAAVFLGIPNALGAVNRCAALKGSGLLTAGTLAGQAAEALDKLRAYGYLPES
                     DLLHTTMYRFATNAIVVTYANAYGRFGVAENLCGFSFANTSAVGVVIPPNPVLEERTF
                     GRGNGIPPTSGVNVVYNDSAGGPLLDLLGVSPSSGLADFSLDGAICHRSLFEGRDIVT
                     GRPLSGDMWDASDRLHDGLFEV"
BASE COUNT          454 a          730 c          805 g          468 t
ORIGIN      
        1 ctggcgcttg actcggcgag tcaagaagag atcaagaagg ctgcggagga tcacgggccg
       61 gagaacctga tcgttgtgtg gggcgtcacc aacgagcaat ttctcgaagt cggttccagg
      121 acggtcatgg agggcgaccc cagttggtcc ggcccgttgg cgggagaggc tttgcacctg
      181 aaggtatttc atgtgctgga accggaaatc aaaaatcaca ttcccccgga agtgtacgag
      241 tcggagttga tgcttctgga acttgcgatt ggtgacgaaa agatcgggaa aacgattgcc
      301 atgatggagg ggttccgcgc tcaacggata accggatgag tctggtttcg ttccggttgc
      361 gcacctcgaa ggtcgaatcc cgcaggctat cttggctgaa agaatttcca atccgcgact
      421 ggggcttgtg accgtcctgt ggccggatta ttgagaccag ggaaaatcct tcatcagatt
      481 cgatgattcc ggttggcgca ccggcctttg caaattcatg ccgcgaaacg tttccgagga
      541 tcggaaccag gcttgggtta taataagaac agtgtttgat tttcgtgact tctatcgatc
      601 aaccggccgt gcgcaaggac gtgtcacatt gcaccgtggg ccgcagggtt cgagaggaat
      661 tccgtttggc cccgtgccag ccgaaaggag gacgatgatg gaaaagttca agcaagccct
      721 tcctcgcaga acgtcgtggg cggcgttcgc gttactcctc ggtttttcgt tcattgcggt
      781 gttatgggcc gtccccgcgc ttgccggcga accgttgaat gtcgggatgg ccggcacgcg
      841 caacgagttc ccggactttc tcgttccggg gtcgctgacc gcgaagtatt acgatggcgg
      901 aaccgacgat ctgttgacgg ccggattggg caagaccggc ctcctggggg tcgaaccatc
      961 ggtggcgaat ccgcaggacg cgtcgcagct tcgacggctc gcgatctgga cgaattaccg
     1021 ggcactgctc gacattgcgc ccgccggagg gtacgggtcg ctgtacggac cgaacgtcga
     1081 gaacgacggc acgatcacgg ccggcgaagg gaagattcca ggctgggaat acatcgccgt
     1141 cgcggatgac gggaccggcc gccggaacgt cacggtgatg gtccagatcc ccgatcgctt
     1201 cgatgcccac aatgcatgca tcgtcaccgc cacgtcctcg ggctcgcgcg gcatctacgg
     1261 cgcgatcagt gcggcggggg agtggggcct caagcagggc tgtgccgtcg cctataccga
     1321 caaggggacg gggaatggtc tcgatgacct caccgccaac ctcgtgaacc ggatcgacgg
     1381 caggctggcc gacgcgcagg tcgccggcaa ggattcgctg ttcacggcga atctgacgga
     1441 cgcgaagcgc gcgtctttca acgcggcgac gccgaaccgg tttgcctaca agcatgccca
     1501 ttcgcagcag aatccggagc gcgattgggg ccggaacacg ttgcaggcgg tcgtctttgc
     1561 cttccatgcc ctcaacgacc ggttcgggac tcccgcgccg caggggggca agcgcatcac
     1621 gctggaccgg cggaacacgc tggtcatcgc gtcgggcgcc tccaatggcg gcggtgccgg
     1681 actcgcggcc gcggagcagg accagcacaa cctgatcgac ggcgtcgcgg tcggcgagcc
     1741 gaacgtgaac acgcccgcgg atgaacatct gacgatccgg cagggtgcga cgacgtttcc
     1801 ggtgttcggc cgcgcgctga tcgactactt cacctacgcc aacctgtacc agccctgcgc
     1861 tgctctagat ccccgtgcgg cgacggcgcc ggctgcggtt ttcctcggga tcccgaacgc
     1921 gctcggcgcc gtgaatcgct gcgccgcgct caagggaagc gggcttctca cggcgggcac
     1981 tctcgcagga caggccgccg aggcgctcga caagctgcgc gcgtatggct atctgccgga
     2041 gagcgatttg ctgcacacca cgatgtatcg tttcgccacg aacgcgatcg tagtgaccta
     2101 tgccaacgcc tacggtcgat tcggcgtcgc cgaaaacctc tgcggtttca gctttgccaa
     2161 cacgagcgcc gtgggcgtcg tcattccgcc gaatccggtg ctcgaggagc gcaccttcgg
     2221 gcgtggcaac gggatcccgc cgacgtcggg cgtcaacgtg gtctacaacg attcggccgg
     2281 tggcccgctc ctggaccttc tgggcgtatc gccgtcaagc gggctcgcgg atttctctct
     2341 ggatggcgcc atttgtcatc gcagcctgtt cgagggtcgt gacatcgtta ccggcaggcc
     2401 gcttagcggg gacatgtggg atgcttcgga ccgcctgcac gatggcctgt tcgaagt
//
LOCUS       DASWAV010000204         1617 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U2665
            k141_690993, whole genome shotgun sequence.
ACCESSION   DASWAV010000204 DASWAV010000000
VERSION     DASWAV010000204.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960316
            Sequence Read Archive: SRR10260007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 1617)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1617)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:47:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,929
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1617
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_690993"
                     /isolate="SMAG_U2665"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.36444 N 132.31889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..407)
                     /locus_tag="VF958_04080"
     CDS             complement(<1..407)
                     /locus_tag="VF958_04080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013667086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low affinity iron permease family protein"
                     /protein_id="HEX9148320.1"
                     /translation="MSGNLRRAWEHFARRATQWTGRPWAFGVALGTVVAWLATGPLFH
                     YSDSWQLVINTGTTVVTFLMVFLIQHAQNKDTRAIELKLNELVAAVAGASNRLIDVEE
                     LSEEELDTLHRHYRKLVAMAKKDIDLTLSHSIE"
     gene            complement(588..1037)
                     /locus_tag="VF958_04085"
     CDS             complement(588..1037)
                     /locus_tag="VF958_04085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9148321.1"
                     /translation="MSPKRRWWLLALVSLIAIAAIGSWGCCSVKCCETKPRNQLVLVP
                     STEMVSPDVIAISWHAHQEIVWKLESGSNIRSVDIRLEGKPKPFAACDVTKEGLCKIA
                     CGNGLCLSGPIDPALPQPREKPYPHYKYEFGKSDSTASADPEIRIDP"
     gene            complement(1047..>1617)
                     /locus_tag="VF958_04090"
     CDS             complement(1047..>1617)
                     /locus_tag="VF958_04090"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX9148322.1"
                     /translation="ALANFQRSLALEPTGLAWGNVGATQYYRGRYSESAAAFEKALSL
                     VPSDYVNWSNLGDALRLIPGSGAKATQAYEKGIALARSELATNPNDAAIHSSLALCLA
                     KTGRAAEAHEHLRISLATEPGRPQLLFNAAVVANRAARPADAVGYLSRAVRAGFSTAV
                     IRREPELANLRNRDDFDDVVKGLRRESSK"
BASE COUNT          261 a          514 c          544 g          298 t
ORIGIN      
        1 tcctcgatcg agtgcgagag ggtgaggtcg atgtctttct tcgccatggc gacgagcttt
       61 cgatagtgcc ggtgcagcgt gtcgagctcc tcctcggaga gctcctcgac gtcgatcagc
      121 cgattcgacg cgcccgcgac cgcggcgacg agctcgttca gcttcagctc gatcgcgcgc
      181 gtgtccttgt tctgcgcgtg ctggatcagg aacaccataa ggaacgtgac gacggtcgtc
      241 cccgtgttga tgacgagctg ccagctgtcc gagtagtgga agaggggacc ggtcgcgagc
      301 cacgcgacga cggtgccgag cgcgacgccg aacgcccacg gtcggccggt ccactgggtc
      361 gctcgacgag cgaagtgttc ccacgcgcgg cgaaggtttc cgctcatctt cagaacctcc
      421 attccattcc gagccgcgcg gcgaatccgg cggcggggaa atagtagggc acgagctcgc
      481 cctggaaatc gggaagcgcg tccccgaact cacgtcgacg gctgcagcga aaagtgccgc
      541 agcccgtcgg cctgccggcg ctcctcttga gcgcggagaa agttgctcta cgggtcgatc
      601 cggatctccg ggtcggccga cgccgtcgaa tccgatttac cgaactcgta cttgtagtgg
      661 ggatatggct tctccctcgg ctgggggagc gccgggtcga tcgggccgga gaggcacagt
      721 ccattcccgc acgcgatctt gcagagccct tccttcgtga cgtcgcacgc tgcgaaaggc
      781 ttgggctttc cttcgaggcg gatgtcgacg ctcctgatgt tcgatccgct ctccagcttc
      841 cacacgattt cctgatgggc atgccacgag atcgcgatga catcgggcga gaccatctcg
      901 gtgctcggga ccagcacgag ctggttgcgc ggcttcgttt cgcagcactt gacgctgcag
      961 cacccccagg atccgatcgc ggcgatcgcg atcaacgaga cgagggccag gagccaccac
     1021 cgacgcttgg gggacatctt ctcctcctat ttcgacgatt ccctccggag tcctttcacg
     1081 acgtcgtcga agtcgtcgcg atttctcagg ttggcgagct cgggctctcg gcggatgacc
     1141 gccgtgctga agccggcgcg gaccgcccgc gagaggtagc caaccgcgtc ggctggtcgc
     1201 gccgcccggt tggccacgac ggccgcattg aagaggagct ggggccggcc tggttccgtg
     1261 gcaagcgaga tgcgcaggtg ctcatgcgct tcggccgcgc gcccggtctt ggcgagacaa
     1321 agtgcaagac tcgagtggat ggcggcgtcg ttagggtttg tcgcgagctc cgagcgggcg
     1381 agcgcgatcc ctttctcgta ggcttgcgtt gccttcgcgc cgctcccggg aatgagccgc
     1441 agcgcgtcgc cgagattgga ccagttcacg tagtccgaag ggaccaggga gagcgccttt
     1501 tcgaacgccg ccgccgactc ggagtatcgg ccccggtagt actgagtcgc tccgacgttc
     1561 ccccaggcga ggccggtcgg ctcgagggcg agcgagcgct ggaaattcgc gagggct
//
LOCUS       DASWAV010000205         2560 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U2665
            k141_730947, whole genome shotgun sequence.
ACCESSION   DASWAV010000205 DASWAV010000000
VERSION     DASWAV010000205.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960316
            Sequence Read Archive: SRR10260007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2560)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2560)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:47:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,929
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2560
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_730947"
                     /isolate="SMAG_U2665"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.36444 N 132.31889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..306)
                     /locus_tag="VF958_04095"
     CDS             complement(<1..306)
                     /locus_tag="VF958_04095"
                     /inference="COORDINATES: protein motif:HMM:NF012695.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HEX9148323.1"
                     /translation="MLLAAGRGVRFRPVTETIPKPLFPYLNVPLVRAHLRRLADSGVR
                     DVGINLHHLGDSIEGHLNDPPPGDSRIRFFREPEILGTAGALANAAEWLRDDDFLVVN
                     "
     gene            complement(343..912)
                     /locus_tag="VF958_04100"
     CDS             complement(343..912)
                     /locus_tag="VF958_04100"
                     /inference="COORDINATES: protein motif:HMM:NF014038.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class IV adenylate cyclase"
                     /protein_id="HEX9148324.1"
                     /translation="MTDAASRLEVEIKVPARDLGAVRNKLRDEGARPLAPAHFESNDL
                     YDRGDGELANRGCALRLRQTDGGSTLTFKGLARFEAGIKTREERETAVSDPEEMERIL
                     AALGIERRFRYEKRREEWELEGCVIALDETPIGDFAEVEGEPPAIRRVLQRLGLDFSQ
                     AILDSYAELYQRRRRHEPSLPKDMVFRNR"
     gene            complement(918..1355)
                     /locus_tag="VF958_04105"
     CDS             complement(918..1355)
                     /locus_tag="VF958_04105"
                     /inference="COORDINATES: protein motif:HMM:NF020977.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HEX9148325.1"
                     /translation="MRGRIESDVRSALKSGEKRRVSTLRLLLSALQNERIHAGRDLTD
                     EESEAVIRRAVKQRREAIDQYSKGGREDLAAAEREELSILETYLPAGLTDEEVEAEVR
                     RIIAEKGFSSARDVGLVMKDLMAAHRGRVDGKRAQEIARRLLA"
     gene            1413..>2560
                     /gene="selB"
                     /locus_tag="VF958_04110"
     CDS             1413..>2560
                     /gene="selB"
                     /locus_tag="VF958_04110"
                     /inference="COORDINATES: protein motif:HMM:TIGR00475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0001514 - selenocysteine incorporation
                     [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="selenocysteine-specific translation elongation
                     factor"
                     /protein_id="HEX9148326.1"
                     /translation="MDRFLVGTAGHIDHGKTALVRALTGIDTDRLPEEKRRGITIDLG
                     FAHAEWEGVRVSFVDVPGHERFVRNMLAGAGGIDAVLLVVAADESVMPQTREHFEIVR
                     LLGIRRGVVAVTKVDRAARELVSVTVSDVREMLRGSALADVDVVTVSARTGEGIAELK
                     SAIVRLAAEDDGADRASRGVRLPIDRAFTIAGFGPVVTGSLVSGTISREQKLELLPRR
                     AGVRVRRVEVHGRERTEARAGARVSVNLAGVDLSDLERGLVLATPGAFATSAVLTVRI
                     ELLPSATVLESGDGVSFHHFSTEARALVRVLVGKRIEPGGPGHAQLRLSVPIAAAPGD
                     RFVIRRLSPVTTIGGGVVLDALAPRLSGRPPAEVAEMLRRLEMGTLRDR"
BASE COUNT          376 a          873 c          876 g          435 t
ORIGIN      
        1 gttcacgacc aggaagtcgt cgtcgcggag ccattccgcg gcattcgcga gcgcgcccgc
       61 agtcccgaga atctccggct ctcgaaagaa gcggatccgg gaatcacccg ggggcggatc
      121 gttcagatgt ccctcgatgg agtcgccgag gtggtggagg ttgattccga cgtctcgaac
      181 tccggagtct gcaagacgac ggaggtgcgc gcgcacgagc gggacgttca agtacggaaa
      241 aagcggcttc gggatcgtct cggtcaccgg gcgaaaccgg actccccggc cagcggcgag
      301 gagcatcgcc ttcaagccgg cctccccgag cgatcccccg tctcagcgat tccgaaacac
      361 catgtctttc ggcagggacg gctcatggcg gcgccggcgt tggtagagct cggcgtagct
      421 gtcgagaatg gcctgggaga agtcgagccc gagccgctgg aggacgcgcc ggatcgcggg
      481 aggctcgccc tcgacttcgg cgaagtctcc gatcggcgtt tcgtcgaggg cgatgacgca
      541 accctcgagt tcccactcct cgcgccgctt ttcgtatcgg aagcgtcgct cgatgccgag
      601 ggcggcgaga atccgttcca tctcttccgg atccgacacg gccgtctccc gctcttcgcg
      661 cgtcttgatc cccgcttcga agcgggccag ccccttgaaa gtgagggtcg agccgccgtc
      721 cgtctggcgc aggcgcaggg cgcagccacg atttgcgagc tctccgtcgc cgcgatcgta
      781 aaggtcgttc gactcgaagt gggcgggggc gagcggccgc gctccttcgt cccggagctt
      841 gttccggact gcaccgaggt cacgcgccgg gactttgatt tcgacttcga ggcggctcgc
      901 ggcgtcggtc attgccgtca cgccagcagc cgccgggcga tttcctgcgc ccgctttccg
      961 tcgacccgtc cccggtgcgc cgccatcagg tccttcatca cgagcccgac gtcgcgcgcc
     1021 gaggagaaac ctttctccgc gatgatgcgg cggacctccg cctccacctc ttcgtcggtc
     1081 agacccgccg gcaaataggt ctcgaggatg gaaagctctt cccgttcggc ggccgccagg
     1141 tcctcgcgcc cgcccttcga gtactgatcg atcgcctcgc ggcgctgttt gaccgcgcgc
     1201 cggatgacgg cttcggactc ctcgtcggtc aggtcgcggc ccgcgtggat tctttcgttc
     1261 tgaagggcgg agaggagaag ccggagagtc gaaacgcgac gcttttcgcc cgacttgagc
     1321 gccgaccgga cgtcactctc gatgcggccg cgcatggcgg aacagtaccg caatcacaac
     1381 attcttcgca ccaccgctgt agccttcacc gcgtggaccg gttcctcgtt ggcaccgcag
     1441 ggcacattga ccacggcaag accgcgctcg tgcgcgcgct caccgggatc gacaccgatc
     1501 gcctgccgga ggaaaagcgt cgcggcatca cgatcgatct cggtttcgcg cacgcggagt
     1561 gggagggcgt tcgggtctcg ttcgtcgacg tgccgggcca cgagcgcttc gtgcgcaaca
     1621 tgctcgccgg cgccggcgga attgacgccg tcctgctcgt ggtggcggct gacgaatcgg
     1681 taatgcccca gacgcgcgag cacttcgaga tcgtgcggct gctcggcatc cggcgcggag
     1741 tggtggcggt gacgaaggtc gaccgcgcgg cccgcgagct cgtgtcggtc acggtctccg
     1801 acgtgcgcga gatgcttcgc ggctccgccc tcgccgacgt cgacgtcgtc actgtgtcgg
     1861 cgcgaacggg cgaagggatc gcggagttga agagtgccat cgtccgcctc gcggccgaag
     1921 acgacggcgc ggatcgcgcg tcccgcggcg tgcgcctgcc gatcgaccgg gcattcacga
     1981 tcgccggatt cgggccggtc gtcaccggca gcctcgtatc gggaacgatc tcccgcgagc
     2041 agaagctcga gctgcttcca cggcgcgcgg gcgtgcgcgt tcgccgcgtg gaggtccacg
     2101 gccgggaacg aaccgaggcg cgcgccgggg cgcgggtgag cgtgaacctc gcgggggtcg
     2161 acctctcgga tctcgagcga ggactcgtcc tggcaacccc cggcgcgttc gcgacgagcg
     2221 cggttctcac ggtgcggatc gagttgcttc cttcggcgac cgtcctcgag agcggcgacg
     2281 gcgtctcgtt tcaccacttc tccaccgaag cacgcgctct cgtgcgcgtg ctcgtgggaa
     2341 agcgaatcga gccggggggg ccggggcacg cgcagctgcg actctccgtc cccatagccg
     2401 ccgctccggg agaccgtttc gtgatccgac ggctttctcc ggtgaccacg atcggcggtg
     2461 gcgtcgtgct cgatgcgctc gcgccccggc tatcgggtcg gccccccgca gaagtcgccg
     2521 agatgctgcg ccgcctcgag atggggaccc tgcgcgaccg
//
LOCUS       DASWAV010000206         1923 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U2665
            k141_731051, whole genome shotgun sequence.
ACCESSION   DASWAV010000206 DASWAV010000000
VERSION     DASWAV010000206.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960316
            Sequence Read Archive: SRR10260007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 1923)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1923)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:47:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,929
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1923
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_731051"
                     /isolate="SMAG_U2665"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.36444 N 132.31889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(161..>1923)
                     /locus_tag="VF958_04115"
     CDS             complement(161..>1923)
                     /locus_tag="VF958_04115"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012546.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HEX9148327.1"
                     /translation="IWTLNLAGGDPSKVTSFPTEINGFTWSPDGRWFLVTSDVFPDCA
                     DVACLDARLKARAKSPTKARVAERLLFRHWDSWKEGLRTHVWKIPSAGGTAVDLTPGD
                     WDAPPFDIGGELAFDVSPDGKEFVYASNHDAVEALSTNSDLWLISFDGGGAARNVTPG
                     NKAFDGTPKFSPDGKWIAYRAQGKPGFEADRFRLVLYDRVSGATRELAQNFDDWVEDY
                     EWAPDSRSILFLSEVKGHGVIERAGITSGLPVEVWRGGGPAQIAPAPDGSVVFSASSL
                     TRPTEIFVLPPSRRGDKAGAARTVVAANQTLLAETEMGAVSERFTKSADGRDLQAWLV
                     KPPGFDATKKYPAVFLIHGGPQGAWSDSWSTRWNPEVFAAYGYVVYAPNPRGSTGFGQ
                     EFVDQISRDWAGRAYDDLMRQADDLESLPFVDKARVGAAGASYGGYMIAWILGHTDRF
                     KALVCHDGVVETRSMALETEELWFPKWDLGGWPWDSEFYEKWNPMRSAEKFHTPTLVV
                     TSEKDYRVPFGQGLQLFTALQVRGVPSKLLTYPDEGHWVLKPGNSRVWHATVMDWLAT
                     YLGGARPDKEALEAVYSTTK"
BASE COUNT          319 a          665 c          638 g          301 t
ORIGIN      
        1 gccgtgtagg acgtcgggag atagagcgcg taggtctgct ccgggtccgc ctcgcacacg
       61 acccgcggga cgatctcccc cgtggggaaa ctctcggctc tcgcagcggg cgccgcgccc
      121 gccggaatca aaacggtaaa gaggaggaga acgccgagag tcacttcgta gtgctgtaga
      181 ccgcctccag agcttccttg tccggccgcg cgcctccgag atacgtggcg agccagtcca
      241 tgaccgtcgc atgccatacc cgcgagttgc ccggcttcag cacccagtgg ccctcgtcgg
      301 gatacgtcag gagcttcgaa ggaacgccgc ggacctggag cgcggtgaag agctgaaggc
      361 cctgcccgaa cggcacgcgg tagtccttct cgctcgtgac gacgagcgtc ggcgtgtgga
      421 acttctccgc cgaacgcatc gggttccact tctcgtagaa ctccgagtcc cagggccagc
      481 cgccgaggtc ccacttcggg aaccagagct cctcggtctc gagcgccatc gatcgcgtct
      541 cgacgactcc gtcatggcac acgagcgcct tgaaccggtc ggtgtggccg aggatccacg
      601 cgatcatgta gccgccgtaa gaggcgcccg cggcaccgac ccttgccttg tcgacgaacg
      661 gcagcgactc gaggtcgtcg gcctggcgca tgaggtcgtc gtatgcgcgg cccgcccagt
      721 cgcggctgat ctggtcgacg aattcctgcc cgaacccggt cgagccccgc ggattcggcg
      781 cgtagacgac gtacccgtat gccgcgaaga cctccgggtt ccagcgcgtc gaccacgagt
      841 cgctccacgc gccctgcggc ccgccgtgga tgaggaagac ggcgggatat ttcttcgtgg
      901 cgtcgaagcc gggcggcttg acgagccacg cctggaggtc gcgcccgtcc gcggacttcg
      961 tgaagcgctc cgacacggcg cccatttccg tctcggcgag cagcgtctgg ttcgcggcga
     1021 cgacggtccg cgcggcaccg gccttgtcgc cacgccggga cggcggcagg acgaaaatct
     1081 ccgtcggtcg cgtgagcgaa ctcgccgaaa agacgacgga accgtcggga gcgggggcga
     1141 tctgggccgg tccccctccc cgccacacct caaccggcaa cccgctggtg atcccggccc
     1201 gttcgatgac gccgtgcccc ttcacctccg acagaaacag gatcgaccgg gagtccggcg
     1261 cccactcgta gtcttcgacc cagtcgtcga aattctgggc gagctcgcgg gtcgccccgc
     1321 tcacgcggtc gtagaggacc aggcggaagc gatccgcttc gaacccgggc ttgccctgcg
     1381 cgcggtaggc gatccacttt ccgtccggcg agaacttcgg cgttccatcg aacgccttgt
     1441 tgcccggggt cacgttgcgc gcggcgccac cgccgtcgaa agaaatgagc cagagatccg
     1501 aattcgtcga gagagcctcg accgcgtcgt gattcgacgc gtagacgaac tctttcccgt
     1561 cgggggacac gtcgaaggcg agctcccctc cgatgtcgaa cggcggcgcg tcccagtcgc
     1621 ccggagtcag gtcgaccgcc gtccctccgg ccgaggggat cttccacacg tgcgtccgaa
     1681 gcccctcttt ccacgagtcc cagtggcgga acaggagccg ctcggcgacg cgggccttcg
     1741 tcggcgattt cgcccgcgcc ttcaagcgcg cgtcgaggca ggcgacgtcg gcgcagtcgg
     1801 ggaagacgtc ggaggtgacg agaaaccacc ggccgtcggg cgaccaggtg aagccgttga
     1861 tctcggtcgg aaaggaggtg accttcgagg gatcgccgcc cgcgaggttc agcgtccaga
     1921 tct
//
LOCUS       DASWAV010000207         3574 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U2665
            k141_211745, whole genome shotgun sequence.
ACCESSION   DASWAV010000207 DASWAV010000000
VERSION     DASWAV010000207.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960316
            Sequence Read Archive: SRR10260007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 3574)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3574)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:47:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,929
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3574
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_211745"
                     /isolate="SMAG_U2665"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.36444 N 132.31889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..675
                     /locus_tag="VF958_04120"
     CDS             <1..675
                     /locus_tag="VF958_04120"
                     /inference="COORDINATES: protein motif:HMM:NF013717.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin dinucleotide binding
                     domain-containing protein"
                     /protein_id="HEX9148328.1"
                     /translation="FDEAWVRMMEGAGWWAPGYRSGEELWKKSQETGGWWDPFYDHGD
                     WERVLRTRSGRFELRPDVLERRPGAAVSIAGEKVEDGSVALVLFEPLPVAGGTGAELP
                     FLQAILDPGSEERWETWAEIHPETAMGLGIRDRDRVVISSPHASIFARARVGPRVLPG
                     AVAVPVGLGKLAGGRWAAGVGANPLVLLSGERELFSGLPDPGATRVRIALAAGHERVV
                     SPQRRG"
     gene            693..1379
                     /locus_tag="VF958_04125"
     CDS             693..1379
                     /locus_tag="VF958_04125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004512035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HEX9148329.1"
                     /translation="MVIDLDRCTACEACVVACQVENNVAPAGPEMAARGRAISWLRLV
                     PFQGGEHGTRPELRLVPLPCMHCERPPCTRVCPVAATQIDREGLVAQVYPRCIGCRYC
                     TTACPYTVRHFNWGRPQWPRPMEQALSPDVSVRPRGVVEKCSFCHHRLQRAKDQARAE
                     GRPLRESDYVPACVETCPAEAMAFGDLDDPRSAVARLSESPRAFRLLEELGTRPKVIY
                     LSKGEWGGEI"
     gene            1366..2703
                     /gene="nrfD"
                     /locus_tag="VF958_04130"
     CDS             1366..2703
                     /gene="nrfD"
                     /locus_tag="VF958_04130"
                     /inference="COORDINATES: protein motif:HMM:NF015851.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NrfD/PsrC family molybdoenzyme membrane anchor
                     subunit"
                     /protein_id="HEX9148330.1"
                     /translation="MERSEFDADERLLRPIERTGRAFWIGTGILLAIGLWGVFAWVTQ
                     LRRGLAVTGLNVPVYWGLYITNFVFFIGISHAGTLISAILRLSHAEWRRSITRAAEVI
                     TVLVLFFGMGCVILDLGRPDRVLNVLKHGNFTSPLLWDVTSVATYLTASTIYLYLPLI
                     PDIAILRDRLPKRRWFYRPLALGWTGTAQQRKLLDRAIAAMAVIVIPIAVSVHTVVSW
                     VFAMTVQPMWHSTIFGPYFVVGAIFSGIGAILIAMAIIRKAYHLENYLKPVHFNNLGI
                     LLLVMALLWFYFTFAEFLTTYYGGEPTHMVVFLSKTEGKFSPVFWTMVVSCFVIPFAL
                     LCNKRTRGTIWGPVAASVSVELGMWLERFLIVVPSLSNPRLPLTAAAYSPSWVEWSLL
                     AGFIATFILLYVLFTKLFPIVSIWEIREGREHSLEEVSERVRSYLPALAEAGK"
     gene            2700..>3574
                     /locus_tag="VF958_04135"
     CDS             2700..>3574
                     /locus_tag="VF958_04135"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HEX9148331.1"
                     /translation="MTGRARRLRPVVWIGLATALAAGEPASAEFGDPIHGQSLFVEKN
                     CVQCHAVRGTGGRIGPDLGRTAVKGSFFELAAGMWNHSPVMTRKMGEAQIARPSFEKG
                     ELADLIAFLYFLNYFDEPGDPKIGKVLFAQKHCIQCHRLGREGGTAGPRLDRLPRGTP
                     PLSIARDLWNHGPVMVPAIRRVGLDVPKFEGSEIVDLFAYLRGQGERRAAREFRSSGD
                     PERGRALYATKGCARCHALFGRGRSIGPDLGRSELRGSVTQLAGRMWNHWPAMSEAME
                     SVGMSPPSFQGDEMA"
BASE COUNT          517 a         1196 c         1222 g          639 t
ORIGIN      
        1 ttcgacgagg cgtgggtccg catgatggag ggggcgggct ggtgggcccc gggctaccgc
       61 tccggcgaag agctctggaa gaaatcgcag gagaccggcg gctggtggga tcccttctac
      121 gaccacggag actgggaacg cgtgctgcgg acccggtctg gccgcttcga gctgcgcccg
      181 gacgtgctcg agcggcgtcc cggggcggcc gtctccatcg ccggcgagaa ggtcgaggac
      241 ggctcggtcg ctctcgtgct gttcgagccg cttccggtgg ccggcggaac cggcgccgag
      301 ctgccgtttc tccaggcgat cctcgatcct ggatccgaag aacgatggga gacgtgggcg
      361 gagatccatc cggagaccgc gatgggactc ggcatccgcg accgcgaccg cgtcgtgatt
      421 tcctccccgc acgcctcgat cttcgcgagg gcgcgcgtcg gaccgcgagt gcttccgggc
      481 gcagtcgccg tcccggtggg actcggaaag cttgccggag gtcgctgggc cgccggagtg
      541 ggagcgaatc cgctcgtcct gctctccggc gaacgggagc tcttctcggg cctgccggat
      601 ccgggcgcga cgagagtccg catcgccctc gccgcgggac acgagcgcgt cgtgtcgccg
      661 caacggaggg gctgagcgtg ccgagatggg gcatggtcat cgatctcgac cggtgcacgg
      721 cgtgcgaagc ctgcgtcgtg gcctgccagg tcgagaacaa cgtcgcgccg gcagggccgg
      781 agatggccgc gagggggcgc gcgatttcct ggctgcgact cgtgccgttc cagggagggg
      841 agcacggcac gcgccccgag ttgcgcctcg tcccgctgcc gtgcatgcac tgtgagcgac
      901 cgccgtgcac gcgggtctgc ccggtggcgg cgacccagat cgatcgcgag ggtctcgtcg
      961 cgcaggtcta tccccgctgc atcggatgcc ggtactgcac gaccgcgtgc ccctacacgg
     1021 tccggcactt caactggggc cgcccgcagt ggccgaggcc gatggaacag gccctgagcc
     1081 cggacgtttc ggtccgcccg cgcggcgtcg tcgagaagtg ctcgttctgt catcaccgcc
     1141 ttcagcgggc gaaggaccag gcacgggcgg aaggccggcc tctgcgtgag agcgattacg
     1201 ttcccgcgtg cgtggagacg tgcccggcgg aagcaatggc attcggggac ctcgacgatc
     1261 cgaggagcgc cgtggcgcgc ctgtccgaaa gccctcgtgc cttccggctg ctcgaagagc
     1321 tcgggacccg acccaaagtg atctatctct cgaaagggga gtggggtgga gagatctgaa
     1381 ttcgacgccg acgagcggct ccttcggccg atcgagcgca ccggtcgcgc tttctggatc
     1441 gggaccggca tccttctcgc catcggactg tggggagtct tcgcgtgggt gacccaactg
     1501 cggcggggac tcgccgtcac cggcctgaac gtgccggtct actggggcct ctacatcacg
     1561 aacttcgtct tcttcatcgg catcagtcac gcggggaccc tcatctcggc gatccttcgc
     1621 ctctcccatg ccgagtggcg gcggtcgatc acccgcgccg ccgaggtgat cacggtcctg
     1681 gtgctctttt tcggcatggg ctgcgtgatc ctcgatctcg gccgccccga ccgggtgttg
     1741 aacgtgctta agcacggcaa cttcacgtcg ccgcttctct gggacgtgac gtcggtggcg
     1801 acgtatctga cggcgagcac gatctatctc tatctgccgc tgatcccgga catcgcgatc
     1861 ctgcgggacc gcctaccgaa gcggcgctgg ttctaccggc cgctggcgct cgggtggacc
     1921 ggaaccgcgc aacagcgcaa gctgctggac agggcgatcg ccgcgatggc ggtcatcgtg
     1981 atcccgatcg ccgtcagcgt ccacaccgtc gtctcctggg ttttcgcgat gacggtgcag
     2041 ccgatgtggc actcgacgat cttcggcccc tatttcgtcg tcggcgcgat cttctccggc
     2101 atcggcgcaa tcctgatcgc gatggcgatc atccggaagg cctatcacct cgagaactac
     2161 ctgaaaccgg tccacttcaa caacctggga atcctgctcc tcgtgatggc cctgctctgg
     2221 ttctatttca cgttcgccga gttcctcacg acctactacg gcggcgagcc gacgcacatg
     2281 gtcgtcttcc tctcgaagac cgaggggaag ttctctcccg tcttctggac gatggtcgtg
     2341 agctgcttcg tgattccgtt cgcgctgctc tgcaacaaac ggacgcgggg cacgatctgg
     2401 gggccggtgg cggcgtccgt ctcggtcgag ctcgggatgt ggctcgagcg tttcctgatc
     2461 gtcgtgccga gcctctccaa cccgcggctg ccgctgaccg cagccgcgta ctcgccgtcg
     2521 tgggtcgagt ggtcgctgct cgccggcttc atcgccacct tcatcctgct ctacgtcctg
     2581 ttcacgaagc tcttcccgat cgtgtccatc tgggagatcc gcgagggacg cgagcattcc
     2641 ttggaagagg tcagcgaacg cgtgcggagc tatctgccgg cgctcgcgga agcgggaaag
     2701 tgaccggacg cgcgcgtcgg ctccgacctg tcgtgtggat cgggctcgcc acggccctcg
     2761 cggccggcga gccggcatcc gccgagttcg gagacccgat ccacggtcag tcgctcttcg
     2821 tcgagaagaa ttgcgtgcag tgccacgcgg tgcgcggcac cggcggccgc atcggccctg
     2881 acctcgggcg caccgcggtc aaaggctcgt tcttcgagct cgccgccgga atgtggaacc
     2941 actcgccggt catgacgcga aagatgggcg aggctcagat cgcgcgcccg tccttcgaga
     3001 agggtgaact cgccgacctg atcgccttcc tgtacttcct gaactacttc gacgagcccg
     3061 gcgatccgaa gatcggaaag gtgctcttcg cgcaaaagca ttgcatccag tgtcaccggc
     3121 tgggccggga aggcggcacc gccgggccgc gtctcgaccg ccttccccgc ggcacgccgc
     3181 cgctttcgat cgcgcgcgac ctctggaacc acgggccggt catggttccc gcaatccgcc
     3241 gtgtcgggct ggacgttccg aagttcgaag gcagcgagat cgtggacctc ttcgcctacc
     3301 ttcgaggaca gggggagcgc cgcgccgccc gcgagtttcg atcgtccggg gatccggagc
     3361 gcgggcgggc cctctatgcg accaaggggt gcgcccgctg tcacgctctt ttcggtcggg
     3421 gccgctccat cggccccgac ctcggtcggt cggagctgcg gggcagcgtc acccagctcg
     3481 ccggccggat gtggaaccac tggcccgcga tgtcggaggc catggagtcc gttggcatgt
     3541 cgccaccgtc gttccagggc gatgagatgg cgga
//
LOCUS       DASWAV010000208         2078 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U2665
            k141_290839, whole genome shotgun sequence.
ACCESSION   DASWAV010000208 DASWAV010000000
VERSION     DASWAV010000208.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960316
            Sequence Read Archive: SRR10260007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2078)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2078)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:47:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,929
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2078
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_290839"
                     /isolate="SMAG_U2665"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.36444 N 132.31889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..774
                     /locus_tag="VF958_04140"
     CDS             <1..774
                     /locus_tag="VF958_04140"
                     /inference="COORDINATES: protein motif:HMM:NF017946.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-2 family transporter protein"
                     /protein_id="HEX9148332.1"
                     /translation="FVRIGFLNMLAYRARYYVGILTYLFNVAVYYFIWRAVFRNSPTV
                     VGLSLAEMITYVAVGWTIRSFYFNEIDRDIAAQVQEGRLAMNLIRPVNFQTVMIFDAA
                     GQSAFRAALFTLPIGVVVALLFHVGPPASALAGVLFLTSALLSFFLVAGLNFLVGLIA
                     IRTKSIMGILRAKYLVLELLSGLLIPTTLFPQPLRSILLASPFPHINFTPATLYLGKA
                     TGGAAARMLLEQAAWTAVLLALGQWVWIRSRHRITIQGG"
     gene            774..1574
                     /locus_tag="VF958_04145"
     CDS             774..1574
                     /locus_tag="VF958_04145"
                     /inference="COORDINATES: protein motif:HMM:NF017946.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-2 family transporter protein"
                     /protein_id="HEX9148333.1"
                     /translation="MKGLRRHARLLGLYFTQYAKVRLEYRADFFSAVFASFLGTAASF
                     AFLLIVFSRVSALKDWKFEEMVFLYGFSLLPLGIFNVFSWNLWMFADRYLIEGRFDRV
                     LLRPVSSMFQVLFESFRLESIESIVTGIVAVVWSSRRLGIRFDAADVLLFSVWAISGG
                     VIYVAIFIGLTATSFWIEDRIGISPPVFNLMQFGRYPLSIYDAWVRFTLSFVIPFAFA
                     SFYPTARFLKRPEYLPEFWAVPIVAAISLSIALSLWKSGVARYHSTGS"
     gene            complement(1556..>2078)
                     /locus_tag="VF958_04150"
     CDS             complement(1556..>2078)
                     /locus_tag="VF958_04150"
                     /inference="COORDINATES: protein motif:HMM:NF019307.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="peptidase dimerization domain-containing
                     protein"
                     /protein_id="HEX9148334.1"
                     /translation="GDVGDKATNSIPTEARVSIDFRLVPDQTPEKVRARVEEFLRGQG
                     YFLVSGGAPDPATRLAHPRVLRVDWGHGYPATRAAMDSPVSRAVVKVLSGAAGAPVVR
                     LPTLGGSGPNYLFEQILGAPVLGVPIANHDNNQHAANENLRIGNLWDGIELYAALMAR
                     LGEEWKSRQEPVE"
BASE COUNT          280 a          720 c          618 g          460 t
ORIGIN      
        1 ttcgtccgga tcggttttct gaacatgctc gcgtaccggg cgcgctacta cgtggggata
       61 ctcacgtacc tcttcaacgt cgcggtctat tacttcatct ggcgtgcggt atttcgcaac
      121 tcgccgaccg tcgtgggcct gtcgctcgcc gagatgatca cgtacgtcgc ggtcgggtgg
      181 accatccggt cgttctattt caacgagatc gaccgcgaca tcgccgcgca ggtccaggag
      241 gggcgcctcg cgatgaacct catccggccg gtgaacttcc aaacggtcat gatcttcgac
      301 gccgcgggac agtcggcgtt ccgcgccgcc ctcttcacgc tgccgatcgg cgtcgtcgtc
      361 gcgctcctct tccacgtcgg cccgcccgct tccgcgctcg ccggcgttct cttcctcacg
      421 tccgccctcc tttcgttctt cctcgtcgca ggtctgaact ttctcgtcgg tttgatcgcg
      481 atccggacga aatcgatcat gggcattctc cgggcgaagt acctcgtgct cgagctcctc
      541 tccgggctgc tcatcccgac gacgctcttc ccccagccgc ttcggtcgat cctgctggct
      601 tccccgtttc cgcacatcaa cttcaccccc gcgacgctct acctggggaa agccacggga
      661 ggcgcggccg cccggatgct cctcgagcag gcggcctgga ccgccgtgct cctcgcgctc
      721 ggacagtggg tctggatccg ctcgcgacac cggatcacga tccagggggg ttgatgaagg
      781 gactccgccg ccacgcgcgg ctcctcggcc tctacttcac gcagtacgcg aaggtgcgcc
      841 tcgagtaccg cgccgacttc ttctccgccg tcttcgcctc gtttctcggg acggcggcct
      901 cgtttgcgtt cctcctgatc gtcttctcgc gggtctcggc tctgaaagat tggaagttcg
      961 aagagatggt cttcctctac ggcttctcgc ttctgccgct ggggatcttc aacgtgttct
     1021 cctggaacct ctggatgttc gcggaccgct acctgatcga aggccggttc gatcgggtcc
     1081 tcctgcggcc ggtgtcgtcg atgttccagg tgctcttcga gtcgttccga ctcgagtcga
     1141 tcgagtcgat cgtcaccgga atcgtcgccg tcgtgtggtc ctcgcggcgt ctcggaatcc
     1201 gattcgacgc ggcagacgtc ctcctcttct cggtgtgggc gatctcggga ggggtaatct
     1261 acgtcgcgat cttcatcggg ttgacggcga cgtccttctg gatcgaggac agaatcggga
     1321 tctcaccgcc ggtcttcaac ctgatgcagt tcggccgcta ccctctctcg atctacgacg
     1381 cctgggtgcg cttcacgctc tcgttcgtca tcccgttcgc gttcgcgtcg ttctatccga
     1441 cggctcggtt cctcaaacgt ccggaatacc ttccggagtt ctgggcggtt ccgatcgtgg
     1501 ccgcgatttc actctcgatc gctctctcgc tgtggaagag cggggtcgcg agatatcact
     1561 cgaccggctc ctgacgcgac ttccactcct ctccgaggcg tgccatcagg gccgcgtaca
     1621 gctcgatccc gtcccacagg ttaccgatgc ggaggttctc gttcgccgcg tgctggttgt
     1681 tgtcgtgatt cgcgatcggg actccgagaa ccggcgcacc caggatttgc tcgaagaggt
     1741 agttcggacc gctgccgccg agcgtcggga gccggacgac cggcgcgccg gccgcccccg
     1801 agaggacctt cacgacggcc cgcgagaccg gagagtccat cgcggcacgc gtcgccggat
     1861 acccgtggcc ccaatccacc cgcaggacgc gcggatgcgc cagtcgggtc gccgggtcgg
     1921 gcgccccgcc gctcacgagg aagtacccct gccctcgcag gaactcttcg actcttgcgc
     1981 ggaccttctc gggggtctga tcggggacga gccggaagtc gatcgacact cttgcttcgg
     2041 tcgggatcga gttcgtcgcc ttgtcgccga catctccc
//
LOCUS       DASWAV010000209         3501 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U2665
            k141_211887, whole genome shotgun sequence.
ACCESSION   DASWAV010000209 DASWAV010000000
VERSION     DASWAV010000209.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960316
            Sequence Read Archive: SRR10260007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 3501)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3501)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:47:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,929
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3501
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_211887"
                     /isolate="SMAG_U2665"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.36444 N 132.31889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            123..1670
                     /gene="pruA"
                     /locus_tag="VF958_04155"
     CDS             123..1670
                     /gene="pruA"
                     /locus_tag="VF958_04155"
                     /EC_number="1.2.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007717527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003842 - 1-pyrroline-5-carboxylate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006562 - proline catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-glutamate gamma-semialdehyde dehydrogenase"
                     /protein_id="HEX9148335.1"
                     /translation="MLPEFRNEQLTDFSVESNRNAFRVALTTVQARLPIEGKNRIGGK
                     KIGATKHFESVNPCDHKQVIGRFPQGTKEDAERAIEAAWNAFPDWSRVAASDRSALLL
                     RIASILRRRKHEFSSMMVLEEAKSWSEADADTAEAIDFCEFYAREMERLAQPQPLTPY
                     PGEKNDLEFIPLGVCAVIPPWNFPLAILTGMTTAALVTGNTVVLKPSSDAAAIATMFL
                     EAAEDAGIPDGVLNFLTGPGSTVGNTLVEHPQTRMIAFTGSRDVGLQISEKSGRVANG
                     QLWIKRTILEMGGKDFILVDETADLDEAASGVVSAAFGFQGQKCSACSRLIVHEKVHD
                     ELLERIVAKTRTLTIGDVRDPKNAVGAVINAGAQKKILEYVDLGRKEGKIVAGGEPGP
                     GDGFFVMPTIVDGVKRGARIAREEIFGPVLAVLTVKDFDEGIAVANDTEYGLTGSLYS
                     RDRERLARGKRELFCGNLYLNRKCTGALVGVHPFGGFNMSGTDSKAGGREYLYLFTQA
                     KAISEKL"
     gene            1674..2855
                     /locus_tag="VF958_04160"
     CDS             1674..2855
                     /locus_tag="VF958_04160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020707537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEX9148336.1"
                     /translation="MRIPAPSRRFDRVGFSEIVKVRNRVMALLAAGQKVHRFEGGEPY
                     MTTPETIKRAMEDALARNQTRYAPSSGIPELRRAILEKVRRRNGIPADDESSIVVNGG
                     MHGLFAAFATLLDPGDEVLMLSPYWTPIVDVITWNGGVPVLVPTERARASGIAATLAR
                     LVTPHTKVLYWNSPNNPTGDVFSRREVEEVCAFVADSGLAMISDEAYEDLIYEGEGHV
                     SPASLPGMFERTLSVFTLSKSYSMTGWRAGYVIAPGRYQTPIATAVLYSTNGVSTPTQ
                     WAALEALSMSEAFLEEWKAGYRRRRDRLVEGLRAAGFELETPRAALYLFPKIPASLGT
                     DSRAAAVTLLDTARVATVPGLVFGPEGEGHLRFSFSVPEKTIEEGVEALMKGVRSPLA
                     T"
     gene            2875..3348
                     /locus_tag="VF958_04165"
     CDS             2875..3348
                     /locus_tag="VF958_04165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9148337.1"
                     /translation="MIRLLRIPGIALLLALAAASTARAASWKGLEPLMSRRADVVRVL
                     GKPTSENASDASMHFATREGEATVFLVTRDFAKLKGWDPASEGTVVQIIVQHEKARDT
                     PKSLGLEGDRRFDREVRGDSVFYRNRKEGIIYIFKAGKLATTIYSPKDGGPGSDD"
     gene            complement(3373..>3501)
                     /locus_tag="VF958_04170"
     CDS             complement(3373..>3501)
                     /locus_tag="VF958_04170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018846026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase"
                     /protein_id="HEX9148338.1"
                     /translation="ETLAEAGPGEQVLFADVDPAVVAKLRARFPALKDRRPSAYRR"
BASE COUNT          618 a         1158 c         1153 g          572 t
ORIGIN      
        1 ccccgaatcg cggggagagg gagtcttgtt tgccgcccct tccccaagtg gcggggagag
       61 ggagtacgcc ttcccttgtt caatcattgt cctttgatag attgcccccg ccggaggtcg
      121 tcttgctccc cgaatttcgc aacgagcagc tgacggattt ttccgtcgag tcgaatcgca
      181 acgcctttcg cgtcgcgctc acgaccgtcc aggcgcgcct gccgatcgag ggcaagaacc
      241 gcatcggcgg gaagaagatc ggcgccacaa aacacttcga atccgtcaac ccgtgcgacc
      301 ataaacaggt catcggccgc ttcccgcagg gcacgaaaga agacgccgag cgtgcgatcg
      361 aggccgcctg gaacgcgttc cccgactggt cgcgcgtggc ggcgtccgat cgctcggcgc
      421 tcctcctccg gatcgcctcg atcctccgca ggcgcaagca cgagttctct tcgatgatgg
      481 tgctcgaaga ggcgaagtcc tggtccgagg ccgacgccga caccgcggaa gcgatcgact
      541 tctgcgagtt ctacgcccgc gagatggagc ggctcgcgca gccgcaaccg ctgacgccct
      601 atccgggcga gaagaacgat ctcgagttca tccccctcgg cgtgtgcgcc gtcatcccgc
      661 cgtggaactt cccgctcgcg atcctcaccg gaatgacgac ggccgctctc gtcaccggca
      721 acaccgtcgt cctgaaaccg tcctccgacg ccgccgcgat cgcgacgatg ttcctcgaag
      781 ccgccgagga tgccggcatc ccggacgggg tcttgaactt cctgacgggc cccggctcga
      841 ccgtcggcaa cacgctcgtc gagcacccgc agacccgcat gatcgccttc acgggatcgc
      901 gcgacgtcgg gctccagatc agcgagaagt ccgggcgcgt cgcgaacggg cagctttgga
      961 tcaagcgaac gatcctcgag atgggaggca aggatttcat cctggtcgac gagaccgccg
     1021 acctcgacga ggccgcgagc ggggtcgtct cggccgcgtt cggtttccag gggcagaagt
     1081 gctccgcgtg ctcgcgactg atcgtgcacg agaaggtcca cgacgagctc ctcgagagga
     1141 ttgtcgcgaa gacgaggacg ctcaccatcg gggacgtccg cgacccgaag aacgcggtcg
     1201 gagccgtgat caatgccggc gcgcagaaga agatcctcga gtacgtcgac ctcggccgca
     1261 aggaggggaa gatcgtcgcg ggcggagagc ccggaccggg agacggtttc ttcgtcatgc
     1321 cgacgatcgt ggacggcgtg aagcgcggcg cccgcatcgc gcgcgaagag attttcggac
     1381 ccgttctcgc agtcctcacg gtcaaggatt tcgacgaggg catcgccgtc gcgaacgaca
     1441 ccgagtacgg actgaccggt tctctctatt cccgcgaccg cgaacgtctc gcccgaggaa
     1501 agcgcgagct cttctgcggc aacctgtatc tgaaccgcaa gtgcacgggc gcgctcgtcg
     1561 gcgtgcatcc gttcggcggg ttcaacatgt cggggaccga ctcgaaggcc ggcggacgcg
     1621 agtacctgta cctctttact caggccaagg cgatctccga aaaactgtag tccgtgagaa
     1681 tacccgcgcc ctcccgccgg ttcgatcgcg tcggcttttc cgagatcgta aaggtgcgca
     1741 accgcgtcat ggcgctgctg gccgcggggc agaaggtcca tcggttcgag gggggcgagc
     1801 cgtacatgac aacccccgag acgatcaagc gcgcgatgga ggatgcgctc gcgcgcaacc
     1861 agacacgcta tgcaccgtcg tcgggaatcc cggagctgcg tcgagcgatc ctcgagaagg
     1921 tgaggcgcag gaacggcatt ccggccgacg acgagagctc gatcgttgtg aacggtggca
     1981 tgcacggcct tttcgcggcg ttcgcgaccc ttctcgatcc gggtgacgag gtgctcatgt
     2041 tgtcgccgta ctggacgccg atcgtcgacg tcatcacgtg gaacggcggc gtgcccgttc
     2101 tcgtgccgac cgagcgagca cgcgcgagcg gaatcgccgc gacgctcgcc cggctcgtta
     2161 ccccgcacac gaaagtcctc tactggaact ccccgaacaa tccgaccggc gacgtgttct
     2221 cgcgtcgcga ggtcgaggag gtctgcgcgt tcgtcgcgga cagcggcctc gcgatgatct
     2281 cggacgaggc gtacgaggac ctgatctacg agggcgaagg gcacgtctcg ccggcttcac
     2341 tccccggcat gttcgagcgc acgctctccg tcttcacgct ttccaagagc tactcgatga
     2401 cggggtggcg cgcgggttac gtgatcgcgc ccgggcggta tcagactccg atcgcgaccg
     2461 cggttctgta ctcgacgaac ggcgtctcga caccaacgca gtgggcggcg ctcgaggcgc
     2521 tctcgatgag cgaggcattc ctcgaggagt ggaaggccgg ctaccggcga cggcgagacc
     2581 gactcgtcga ggggttgcgc gcggccgggt tcgagctcga gacgccgcgc gcggcgctgt
     2641 atctcttccc gaagatcccg gcgtcgctcg ggacggactc gcgcgccgcg gcggtcacgc
     2701 tcctcgacac ggcgcgggtg gcgacggtgc ccggcctggt gttcggcccg gagggagagg
     2761 ggcacctgcg cttctcattc tctgtccccg agaagacgat cgaggaggga gtcgaggcgc
     2821 tcatgaaagg ggtcaggtct ccacttgcca catgacaccc agaatgagcg ccggatgatc
     2881 aggctgctcc gaatccccgg aatcgcgttg ctcctcgccc tcgccgccgc ctccactgcg
     2941 cgtgcggcga gctggaaggg tctcgagccg ctcatgtccc gccgtgcgga cgtcgttcgc
     3001 gtgctcggaa agccgacgag cgagaacgcc tccgacgcat cgatgcattt cgcgacgcgc
     3061 gagggagagg cgaccgtctt cctcgtgacg cgcgacttcg cgaagctgaa aggctgggat
     3121 cccgcctccg agggcaccgt cgttcagatc atcgttcagc acgagaaagc gcgggacacg
     3181 ccgaagtcgc tcgggctcga aggagatcgg cggttcgatc gtgaagtgcg cggcgactcg
     3241 gttttctatc ggaaccggaa agagggaatc atctacatct tcaaagccgg caagctcgcc
     3301 accaccatct actcgccgaa ggatggcggt ccgggatcgg acgactaggg cgggagccct
     3361 gaccccgacc ttctacctcc ggtacgcgga cggccggcgg tccttcaggg ccgggaaacg
     3421 cgcgcgcagc ttcgcgacca cggcgggatc cacgtcggcg aagagcacct gctcgcccgg
     3481 gcccgcctcc gcgagcgtct c
//
LOCUS       DASWAV010000210         4559 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U2665
            k141_291019, whole genome shotgun sequence.
ACCESSION   DASWAV010000210 DASWAV010000000
VERSION     DASWAV010000210.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960316
            Sequence Read Archive: SRR10260007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 4559)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4559)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:47:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,929
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4559
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_291019"
                     /isolate="SMAG_U2665"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.36444 N 132.31889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..215)
                     /locus_tag="VF958_04175"
     CDS             complement(<1..215)
                     /locus_tag="VF958_04175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9148339.1"
                     /translation="MKKAVWVGVAIAIAVAGTWGYGALARREDDSGRPVRLLAIAEEQ
                     SLALSFSRSGRLISPVPDEGETVKKGNT"
     gene            332..838
                     /locus_tag="VF958_04180"
     CDS             332..838
                     /locus_tag="VF958_04180"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX9148340.1"
                     /translation="MSSRPSKPSNPVGQPKRILVVEDDGSYRWVIRRLLESAFGDEIS
                     VEEAPTGDRAIERLGSGAPLDLVLLDLSLPGADGFAILERLRADERTRLVPVIVVTSS
                     QEQSDVRRAYELGANSFVSKSDSPEKTLQRLRLLPIYWLELNRLPEEDPSDRNRASSR
                     SSTSSARP"
     gene            complement(745..1533)
                     /locus_tag="VF958_04185"
     CDS             complement(745..1533)
                     /locus_tag="VF958_04185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018125753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent deacylase"
                     /protein_id="HEX9148341.1"
                     /translation="MASEGLTPLVRRLAEAKRVTVITGAGVSAASGVPTFRGADGLWS
                     NFRVEDLATAAAFARDPQLVWKWYAWRRELLAACRPNRAHEVLAEWSRRYPKFTLITQ
                     NVDGLHERAGTENVVRFHGSLWDLQCWNGCPDSPPSWRDERVPFPELPPRCPHCAGLA
                     RPGVVWFGEAIDPAVLAASLAALDCDICIVVGTSAVVYPAAGLADEARRRGAFTVEIN
                     PEKTEATDRFDLSLQGRAEDVLDRLDARLRSEGSSSGNRLSSSQ"
     gene            complement(1736..2098)
                     /locus_tag="VF958_04190"
     CDS             complement(1736..2098)
                     /locus_tag="VF958_04190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002610823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX9148342.1"
                     /translation="MSRQVLVVEDDPELLGSLASFLQGEGYTVECARHGLEALGRLRG
                     GCRPDVILLDLMMPIMTGWEFRDAQRQDSELSRIPVIVVSGMNDSEQHAAWLEADGYV
                     RKPIPVETLIETLNRYCD"
     gene            complement(2221..3627)
                     /locus_tag="VF958_04195"
     CDS             complement(2221..3627)
                     /locus_tag="VF958_04195"
                     /inference="COORDINATES: protein motif:HMM:NF024853.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HEX9148343.1"
                     /translation="MGKRERRVAEARERRQSAEAGADALPPVVGPRSLAKLIVLGALS
                     ALWSVVLWEELVLVRSGGSSFCGFGGKFDCAAVWNGAFASAVHGFTGLPIAGWGLAWS
                     AVAFFLPLVALFRSAEGKPIDPLVSAVRLTALAGILSVAVMIAESAIAGAICIGCIGT
                     YVLVGAYAFIALLQWRPAGFPAARRGATIAAVAAVAAFLLLLFPGLRTPKTSGEAGRK
                     AVEEVARQEAGGPVAVGTGDARRDEKLTELVNSLEPPLKQTLADSLAIYRASATFTPP
                     PPRALVGSDLAPVRITEFTDILCEHCATLHETLKTLRESLPPGSFNVDSRQFPLDSRC
                     NPLVGGSREDDVRCVAAKARICLEPSGNEPKLADALFARQKGLTREKVFEIAAPFMPR
                     SALEACIDAPTTRGMLESDIAAASHYDSDGTPIVVVNGKKGTSFGPFLYAIIQTRGSA
                     QHPAFAVLPPGDPSAHLH"
     gene            complement(3649..4113)
                     /locus_tag="VF958_04200"
     CDS             complement(3649..4113)
                     /locus_tag="VF958_04200"
                     /inference="COORDINATES: protein motif:HMM:NF019932.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HEX9148344.1"
                     /translation="MSSLRLRLACPLLLAALAVPVPPPAPDATRELEKRERDLVSAIG
                     RGDLATYDRIVADDYVAFEASGKTSAKAEIMASYRTGARKYTDLAIFDVEARIFGDTA
                     VVTAKTKGFRREGGRDVPNRVRYIRVYARRNGEWRAVAQMAAPLPPGEPSER"
     gene            complement(4131..>4559)
                     /locus_tag="VF958_04205"
     CDS             complement(4131..>4559)
                     /locus_tag="VF958_04205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003634973.1"
                     /note="seems to be involved in biofilm formation; in
                     asymptomatic bacteriuria E. coli strains 83972 and VR50,
                     the ygiD gene is upregulated during biofilm formation in
                     urine; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4,5-DOPA dioxygenase extradiol"
                     /protein_id="HEX9148345.1"
                     /translation="VVQLSIDELQPPSFHFEIGRKLAALREEGVLVVGSGNLVHNLHA
                     YAWGRHVPEPYDWAVRFESEVKGLLLAGDHKPLVDYETLGREALLSVPTPDHYLPLLY
                     VIGARQGDDVVTFPVEGVDGGSISMLTVQVGAPLRDLTAP"
BASE COUNT          749 a         1586 c         1506 g          718 t
ORIGIN      
        1 gtgttgcctt tcttgacggt ctcgccctcg tccggcacgg gactgatcag gcgtccgctg
       61 cgagagaagg aaagagcaag gctctgttcc tccgcgatgg ccagaagccg gacagggcgc
      121 cccgagtcgt cttcccggcg cgcaagcgcg ccgtaacccc aggtgcccgc cacggcgatg
      181 gcgatcgcca cgcccaccca cacggctttc ttcatcggcc cagcggtcgg cgctgagttt
      241 aacgccattt cggccgtggc gatttgagga cagccctcgg agggcggtat cagtcttgct
      301 ctctcaactc ctttttcgag gagcagagcc cttgagcagc agaccgtcga aaccgtcgaa
      361 cccggtcgga cagccgaagc gcatcctcgt cgtcgaggac gacggaagct accgctgggt
      421 catccggcgc ctgctcgaga gcgcgttcgg cgacgaaatc tccgtcgaag aggcgcccac
      481 gggcgaccgg gcgatcgaac gcctcggctc cggcgctccg ctcgacctcg tcctcctcga
      541 tctctcgctt cccggcgcgg atgggttcgc catcctggag cgcctccgcg cggacgagcg
      601 gacccgtctc gtaccggtca tcgtcgtgac gagctcgcag gagcagagcg acgtgcgccg
      661 cgcctacgag ctcggagcga actcgttcgt ctcgaagtcc gactcgcccg agaagacgct
      721 tcagcgcctt cggctgctcc ccatctactg gctggagctc aaccggttgc cggaagagga
      781 cccttccgac cgcaaccggg cgtcgagccg gtcgagcacg tcctccgcgc gtccctgaag
      841 tgacagatcg aagcgatccg tcgcttcggt tttttccggg ttgatctcga cggtaaaggc
      901 accgcggcgc cgcgcctcgt ccgcgagccc cgccgccggg tagacgaccg cggaggtccc
      961 gacgacgata cagatgtcgc agtcgagcgc cgcgagtgac gcggcgagaa cggcggggtc
     1021 gatcgcctcc ccgaaccaga cgacccccgg gcgcgcaagc cccgcgcagt gcgggcatcg
     1081 gggcgggagc tccggaaacg gaaccctctc gtcgcgccag ctcggcggcg agtcggggca
     1141 gccgttccag cactgcaggt cccaaagcga gccgtggaag cgcacgacgt tctctgtccc
     1201 cgcccgttcg tggaggccgt cgacgttctg cgtgatcagc gtgaacttgg ggtagcgccg
     1261 gctccactca gcgaggactt cgtgcgcgcg gttgggccga cacgcggcga ggagctcccg
     1321 ccgccacgcg taccacttcc agacgagctg cgggtctcgc gcgaacgcgg cggcggtcgc
     1381 gaggtcctcg acgcggaagt tcgaccacag gccgtccgcc ccgcgaaagg tcggcacgcc
     1441 gctcgccgcc gaaactccgg cgccggtgat cacggtgacg cgtttggctt cggcgagccg
     1501 tctgacgagt ggagtcagac cttcacttgc catttcggag aacgattctc acgcatcggg
     1561 gcacgccgcg aggagtctgt ggctcgcggc gcgaggagtc tcctgaggta atcgaaggag
     1621 gagcctcgcg ccgcgggagc gcgaccgtag cgaacgacca ggcacgcggt cgaagccggc
     1681 ctgctgagcc gcgtgccggt gcggaaagca ggtcgttcgc aagggagcgc gacggtcagt
     1741 cgcagtagcg gttgagcgtc tcgatcagag tctcgacggg gatcggcttc cgaacgtagc
     1801 cgtcggcctc gagccaggcg gcgtgctgct cgctgtcgtt catccccgac acgacgatga
     1861 ccgggatgcg tgagagctcc gagtcctgac gctgagcgtc gcggaactcc caccccgtca
     1921 tgatcggcat catcaggtcg agcaggatca cgtcgggccg gcatccgccg cgcaggcgtc
     1981 cgagcgcctc gaggccgtgg cgcgcgcact cgaccgtata gccctccccc tgaaggaacg
     2041 aggcgagcga gcccagcagc tccggatcgt cttccacgac gagcacctgg cggctcatgt
     2101 ctcgattctc gtcccggcgg tccgaagaaa gagaatccct gacgtcggag acattctcca
     2161 ctcgaagggt gcaagacctc tgccgtgtcc tcgagcgagg acgacccctg cggccgccga
     2221 tcagtgcagg tgcgcgctcg gatcgcccgg gggaagaacg gcgaacgcgg ggtgttgcgc
     2281 actgccccgc gtctggatga tcgcgtacag aaacggaccg aagctcgttc cctttttgcc
     2341 gttgacgacg acgatcggcg tgccgtcgga gtcgtagtga gaggccgcgg cgatgtcgct
     2401 ttcgagcatg ccgcgcgtcg tgggagcgtc gatacacgcc tccagcgcgg accgcggcat
     2461 gaagggcgcc gcgatctcga aaaccttttc ccgggtcagc cccttctgcc gggcgaacaa
     2521 cgcatcggcg agcttcggct cgttgccgga cggttccagg cagatcctgg ctttcgcggc
     2581 cacgcatcgg acgtcgtcct cccggctgcc gccgaccagg ggattgcagc ggctgtcgag
     2641 cgggaactgc cgggaatcca cgttgaagct tccgggcggc agggattcgc gcagggtctt
     2701 caacgtctcg tgaagggtcg cgcagtgctc gcagaggatg tccgtgaact ccgtgatgcg
     2761 caccggcgcc aggtccgagc cgaccagggc ccgcggcggc ggcggcgtga acgtggccga
     2821 agctcgatag atcgccagcg agtccgcgag cgtctgcttg agcggcggct cgagagaatt
     2881 cacgagttcc gtcaacttct cgtcgcggcg cgcgtcaccg gtacccaccg cgaccggtcc
     2941 gcccgcttcc tggcgcgcga cttcctccac cgccttccgc cccgcctcac cgctcgtctt
     3001 cggcgttcgc aaccctggga agagcagaag gagaaaggcc gcgaccgcgg cgacggccgc
     3061 gatcgtcgca ccgcggcgcg ccgccgggaa ccccgcggga cgccactgca gcagcgcgat
     3121 gaacgcgtag gctccgacga ggacgtacgt cccgatgcac ccgatgcaga tggcgccggc
     3181 gatcgcgctc tccgcgatca tcacggccac tgacaggatg ccggcgagcg ccgtcagtcg
     3241 cactgcggaa accagcggat cgatgggctt gccttccgcg ctgcgaaaca gtgcgacgag
     3301 cggcagaaag aacgccactg cgctccacgc caggccccag ccggcgatcg gcagtcccgt
     3361 gaacccgtgg accgcggacg cgaacgcacc gttccagacg gccgcgcagt cgaacttgcc
     3421 gccgaagccg cagaaggagc tgccgcccga acgcacgagg acgagttcct cccacaggac
     3481 gaccgaccac agagctgaca gcgcgccgag gacgatgagc ttcgcgagac tcctcggccc
     3541 gaccactggc ggcagcgcat cggctcccgc ttccgcggat tgccgccgct ctctggcctc
     3601 tgcgacccgg cgttcccttt tacccatgcg gctcggagtt tgacacgact atctctccga
     3661 cggttcgccg ggcgggagcg gagccgccat ctgcgccacg gctctccatt ctccgttccg
     3721 cctcgcgtag acgcggatgt agcgcacccg gttcgggacg tcgcggccgc cttctctgcg
     3781 gaagcccttc gtcttcgccg tcacgaccgc cgtgtcaccg aagatccggg cctcgacgtc
     3841 gaaaatcgcg aggtcggtgt atttcctcgc tcccgtccga taggacgcca tgatttcggc
     3901 cttggcgctc gtcttgccgg acgcctcgaa ggcgacgtag tcgtcggcga cgatcctgtc
     3961 gtacgtcgcg aggtcccccc tgccgatcgc cgagacgaga tcccgctcgc gcttttccaa
     4021 ctcgcgcgtc gcgtccggcg ccggaggcgg cactggcacg gcgagcgccg ccaggaggag
     4081 ggggcaggcc agacgtaatc gcagggaact catcaatgaa gcctgtcctg ttacggcgcg
     4141 gtcaaatccc gcaacggcgc cccgacctgc accgtgagca tcgaaatcga tccgccgtcc
     4201 acgccttcga ccggaaaggt gaccacgtcg tccccctgtc gggcgccgat gacgtacagc
     4261 aggggcagat agtgatcggg cgtcggaacc gagagcaggg cctctcgccc cagtgtctcg
     4321 taatccacga gcggcttgtg gtctccggcg aggagcagtc ccttcacttc gctctcgaag
     4381 cggaccgccc aatcgtatgg ctccggcacg tgccgtcccc atgcataggc gtgcaggttg
     4441 tggacgaggt tgccgctgcc cacgaccagg acgccttcct ctctcagcgc ggcgagcttt
     4501 cgaccgatct cgaagtggaa ggaggggggc tgcaactcgt cgatgctcaa ctgcaccac
//
LOCUS       DASWAV010000211         6365 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U2665
            k141_530974, whole genome shotgun sequence.
ACCESSION   DASWAV010000211 DASWAV010000000
VERSION     DASWAV010000211.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960316
            Sequence Read Archive: SRR10260007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 6365)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6365)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:47:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,929
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6365
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_530974"
                     /isolate="SMAG_U2665"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.36444 N 132.31889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..618)
                     /gene="tatC"
                     /locus_tag="VF958_04210"
     CDS             complement(<1..618)
                     /gene="tatC"
                     /locus_tag="VF958_04210"
                     /inference="COORDINATES: protein motif:HMM:TIGR00945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatC"
                     /protein_id="HEX9148346.1"
                     /translation="MKLVGPASQDTGEGLELGRLRGAEDELPRMGFFEHLEEFRRRLI
                     ASLIALAAAFGIAWSRAEWLFDLLARPVRRFLPPGQNLAYTTLTEPFLLYFRVALLGG
                     LILASPIILWQLWLFIAPALYRREKRLAIPFLFASVFFFLSGCAFGYLEAFPVVVGFL
                     VGMGSNFQAVITINEYLSTASKIILGLGLCFELPIVIFFLTRMGVV"
     gene            complement(647..1033)
                     /gene="tatA"
                     /locus_tag="VF958_04215"
     CDS             complement(647..1033)
                     /gene="tatA"
                     /locus_tag="VF958_04215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010940700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase TatA/TatE family
                     subunit"
                     /protein_id="HEX9148347.1"
                     /translation="MFGPIGMPELILIFLVALLVFGPRKLPELGKSLGRGLAEFKRAS
                     EDLKRTIEDEIEQGKNQVSTVRDELAAVREQAGELRNTIASTGNVGVTPSASAQPPAP
                     AEPPAPVQTPETRAEDAVPSAPIRPA"
     gene            1139..2752
                     /locus_tag="VF958_04220"
     CDS             1139..2752
                     /locus_tag="VF958_04220"
                     /inference="COORDINATES: protein motif:HMM:TIGR00225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HEX9148348.1"
                     /translation="MSPIRSRFSIAVLLLAVSTLAGGAFAAIVFASPERESPPLDEYA
                     DILTTLTEWSPQPIPPEQFVYASVHGMLSRLDPHTAFLEPEDFSAMREKQQGSFFGLG
                     IQIQKRMGHITVIAPMEGTPAYKLGIRAGDVITHIEDEEIKEDVTTDEVVRKLRGPKG
                     SQVTITIRRAGLDEPIKMTITRAEIPTYSVRYAFMLTPEVGYVLLADFTHTSSRELKA
                     AIARLDKQGMKKLLLDLRGNPGGVLEQSVDVADVFLEHGSKVVYTRGRTSSSAQDYLA
                     PGDGYHFDKPLVVLVNRGSASASEIVAGAIQDHDRGLIVGQRTWGKGLVQSVYTLPYG
                     AGLALTTAKYYTPSGRWIQRDFTDLLAYVNPDDADAGGEEAAETPKKEGETFYTDAGR
                     VVYAAGGITPDMAVKNNRESKLLTQVLARNLPFNFAVEWLVKHPDVSRDVTVTPAMRD
                     EFFRFAEASKFSTVEELKRMWNEDPNRQLVDLAIKIELVNAKYGLEAGWAVRATGDTQ
                     IQTAMTQFGEAERIASLPKKKNPARASKM"
     gene            complement(3051..4745)
                     /locus_tag="VF958_04225"
     CDS             complement(3051..4745)
                     /locus_tag="VF958_04225"
                     /inference="COORDINATES: protein motif:HMM:NF037015.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HEX9148349.1"
                     /translation="MLRKSPGLLALLFVGAGALAPSISAQPIGVIDQPTQGQRVSGVV
                     KVTGWVLDFNAIDRVELLVDGTFANRADTNLPRADVLEVFPTYVNSANPNPGFVTSFL
                     ARRYPDGAHALSIRVTEASQATFLIGPITVIIDNTVNQAPFGFIDIPGPSGVEGTDGP
                     LPVSGWAIDDEDVDHVDFLMDGQIVAGAVGRGLPSTAIYGTTRPDVAAAFPDVPNSLY
                     SGFQANVDTSKLINGVHQLSVRVTDIRGASRVIGTRTIQVINNGANLAPFGQIDFPLD
                     KSNLFCTSIDVGIPSPCTPDVCRFNLTNIVAGWALDVGARLDAGQVSFVELLLDGAII
                     ANTRTDCVQFGHSFVNCYGINRPDVARNYSGYVNADNAGFLFSFALFRDATDPTGLIQ
                     IRLPGADPAAEPPIVGFTRPGKHTLAVRVGDEEETVTQLGAMSVDVFCDPSGADFPAF
                     GYIDTPSDYQFINGIFDVFGWAFDFNGVQRIEVDVDGQVVGNAAYGLSRPDVPATDPR
                     APSVSVGFSYLLDTTRLSDSEHDLVIYVVDQQFGTPGRTEIGRRKFVVNNNALTHR"
     gene            4868..5929
                     /locus_tag="VF958_04230"
     CDS             4868..5929
                     /locus_tag="VF958_04230"
                     /inference="COORDINATES: protein motif:HMM:TIGR00374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase transmembrane
                     domain-containing protein"
                     /protein_id="HEX9148350.1"
                     /translation="MTSQVAPNSGPSRSKRALKSRPIQIGLSVILAAALLVFFLRGIN
                     FRDIANQVAAASGGWLGASVVLAIGTFLFRALRWTWLLRPVARVRFYPAFVATAFGFA
                     ANNLPGKVGEVLRPALLARSEKLPFSPLLASVLLERVFDGAAVLFFFVIAAATGIPNT
                     HGTGKLLLPAGLLLVLVGAVLFSVFWRVETERFLEKFWRRLPERFQPRARTFAETFVD
                     GFASLKNPKLLLLVTFGSLAMWLIINLQIYAVLRAFHLDLPFSASFVVTAAAVLGLMF
                     PTPGGLGSYHLAVQIALTDVYGVAKDTASGVALLAHAISFVPITLLGLALFAMTPIRG
                     KQIEELERITETGMEKREP"
     gene            5985..>6365
                     /gene="nrdR"
                     /locus_tag="VF958_04235"
     CDS             5985..>6365
                     /gene="nrdR"
                     /locus_tag="VF958_04235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011682570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HEX9148351.1"
                     /translation="MKCPFCGAVDDRVVDSRESREGEVIRRRRECESCGRRFTSYETI
                     EEIPYMVVKKDGRREHFDRKKLRAGLVKACEKRPVSPAQLDTIVDEIETQLHDTEERE
                     MSTSDIGARVMDRLRELDKVAYVRF"
BASE COUNT         1151 a         2048 c         2081 g         1085 t
ORIGIN      
        1 gacgaccccc atccgcgtca ggaaaaagat cacgatcggc agctcgaagc agaggccgag
       61 tccgaggatg atcttcgacg ccgtcgagag gtactcgttg atcgtgatca ccgcctggaa
      121 gttcgacccc attccgacga ggaagccgac gaccaccgga aacgcctcga gatagccgaa
      181 ggcgcagccg ctcagaaaga agaagacgct cgcgaagaga aacgggatcg cgagccgctt
      241 ctcccgccgg tacagagccg gagcaatgaa cagccagagc tgccagagga tgatcgggga
      301 cgccagaatc aatccaccga ggagcgcgac ccggaagtag aggaggaagg gctcggtcag
      361 ggtcgtgtag gcgaggttct gcccgggcgg caggaagcgg cgcaccggcc gcgcgaggag
      421 atcgaacagc cactcggccc gcgaccacgc gattccaaaa gcggcggcga gcgcgatcaa
      481 cgaagcgatg aggcgccggc ggaactcttc gaggtgctcg aagaacccca ttcgcgggag
      541 ctcgtcctct gcgccacgca ggcgaccgag ttcgagacct tcgccggtat cctgcgaagc
      601 ggggccgacg agcttcacgg tctcgccccg cgccggcgtg tgcccgtcaa gcgggccgga
      661 tcggggccga cgggacggcg tcttccgcgc gtgtttcggg cgtttgaacc ggggccggcg
      721 gctcggccgg ggcgggcggc tgagctgagg cagagggcgt cacaccgaca ttgcccgtgg
      781 aggcgatggt gttgcgcaac tcccccgcct gctcccgtac ggccgcgagc tcgtcccgga
      841 ccgtcgagac ctggttcttt ccctgctcga tctcgtcctc gatcgtgcgt ttcaggtcct
      901 cggaagcgcg cttgaactcg gcaagccccc gcccgagcga ttttccgagc tccgggagct
      961 tccgaggccc gaaaacgaga agggcgacga gaaatatcag gatgagctcg ggcattccta
     1021 tggggccaaa catgttcgtt tactccgtga gtgattctac gctcgaaggt ccgtcggaat
     1081 tcccgcaccg ggggttcggg cgtcctcatg tcacaatgac gctccgagga ggaaccgagt
     1141 gagcccgatc cggtcccgtt tttccatagc cgttctgctc ctggccgttt cgacgctggc
     1201 cggaggcgcc ttcgcggcga tcgtcttcgc ctctcccgaa agggagtcgc cgccgctcga
     1261 cgagtacgcg gacatcctga cgactctcac cgaatggtcg ccccagccga tcccgccgga
     1321 gcagttcgtg tacgcctcgg tccacggcat gctctcccgg ctcgatcccc acaccgcttt
     1381 cctggagccg gaggacttct cggccatgcg ggagaagcaa cagggctcgt tcttcggtct
     1441 gggcatccag atccagaagc ggatgggcca catcacggtg atcgcgccca tggagggaac
     1501 gcccgcctac aagctcggga tccgcgccgg agacgtcatc acgcacatcg aagacgagga
     1561 gatcaaagaa gacgtcacca ccgacgaggt cgttcggaag ctgcgcggtc ccaagggcag
     1621 ccaggtgacg atcacgatcc gacgggcagg attggatgaa ccgatcaaga tgacgatcac
     1681 gcgcgcggag atcccgacct actccgttcg ctacgcattc atgctgacgc ccgaggtcgg
     1741 ctacgttctt ctcgccgatt tcacgcatac ctccagccga gaactcaagg ccgcgatcgc
     1801 gcggctcgac aagcagggca tgaaaaagct cctgctcgac ctgcgaggca accccggggg
     1861 cgtgctggag cagtcggtgg acgtcgccga cgttttcctc gagcacggct ccaaggtcgt
     1921 ttacacgcgc ggccggacga gctcctcggc tcaggactac cttgcgcccg gggacggata
     1981 ccacttcgac aagccgctcg tcgttctcgt caaccggggc tcggcatccg cctcggaaat
     2041 cgtcgccggg gccatccagg atcatgaccg cggattgatc gtcggacagc gaacctgggg
     2101 gaaagggctc gttcagagcg tttacaccct tccgtacggc gccgggctcg cgctgactac
     2161 cgcgaagtac tacaccccgt cgggccgctg gatccagagg gacttcacgg atctcctggc
     2221 ctacgtgaac ccggacgacg cggatgcggg cggcgaggag gccgccgaga cgccgaagaa
     2281 ggagggcgag accttctaca ccgacgcggg acgtgtggtc tacgcggccg ggggcatcac
     2341 ccccgacatg gcggtcaaga acaaccgcga atccaagctt ctgacccagg tgctggcgcg
     2401 gaacctcccc ttcaacttcg ccgtcgagtg gctggtcaag caccccgatg tgtcacgcga
     2461 cgtcacggtg acgccggcga tgcgcgatga gttcttccgt ttcgccgagg cgtcgaagtt
     2521 ctcgaccgtc gaagagctga agcggatgtg gaacgaggac ccgaaccggc agctcgtgga
     2581 tctcgcgatc aagatcgagc tcgtgaacgc gaagtacggc ctggaggcgg gctgggcggt
     2641 ccgcgcgacg ggcgataccc agatccagac ggcgatgacg cagttcggcg aagcggagcg
     2701 gatcgcctca ctcccgaaga agaagaaccc ggcgcgcgcc tcgaagatgt agcccgacct
     2761 cagcaccttc ggttcgcggc ggaagaccgg gttatccccg gtctttcgtt tttcgctgcg
     2821 cgaggcgtct cccgaagctc cgggcgcggc gcgaggagtc tcccggttcg catcgcgaag
     2881 agtctcccga actccggggt cgcggcgcga ggagtctccc gaggtaatcg agggaggagc
     2941 ctcgcgccgc gggagcgggc gcgaagcgag ggatcaggcg agcggtcgag cccgccccgg
     3001 cgagccgctc gccggtgcga agcagatccc gagcgagcgc ccgcgacgtt ctaccgatgc
     3061 gtcagcgcgt tgttgttgac gacgaacttc cggcggccga tctccgttcg gcccggcgtc
     3121 ccgaactgct ggtcgacgac gtagatcacg aggtcgtgct cggagtcgga gagccgcgtc
     3181 gtatccagca gatacgagaa cccgaccgaa accgaggggg cgcgcgggtc ggtggccgga
     3241 acgtccggcc tggacagacc gtacgccgcg tttccgacga cctgcccgtc gacgtcgacc
     3301 tcgattcgct ggactccgtt gaagtcgaac gcccacccga acacgtcgaa gatcccgttg
     3361 atgaactggt agtccgacgg cgtgtcgatg tagccgaatg ccggaaagtc ggcgccgctc
     3421 gggtcgcaga agacgtccac cgacatcgct ccgagctggg tcacggtctc ttcctcgtcg
     3481 ccgacgcgaa ccgcgagcgt gtgcttgccg ggtcgggtaa agcccacgat agggggctcg
     3541 gcggccggat cggcgccagg caacctgatt tggatcaatc cagtgggatc ggttgcgtca
     3601 cggaacaggg cgaacgagaa gaggaatccg gcgttgtccg cgttcacgta cccggagtag
     3661 ttgcgggcca cgtcgggtcg gttaatgccg taacagttca cgaacgaatg gccgaactgc
     3721 acgcagtccg tgcgggtgtt cgcgatgatc gcaccgtcca ggagaagctc gacaaaggaa
     3781 acctgtcccg cgtcgagccg tgcccccacg tcgagagccc agccggcgac gatgttggtc
     3841 aggttgaatc tgcacacatc cggcgtacag ggtgacggaa ttcccacgtc gatcgacgtg
     3901 cagaacaggt tggacttgtc gagcggaaag tcgatctgcc cgaacggagc gaggttcgcc
     3961 ccgttgttga tgacctggat ggttcgggtt ccgatcaccc gcgatgcgcc gcggatgtcc
     4021 gtcacgcgga cgctcaactg gtggacgccg ttgatgagct tgctcgtgtc cacgttcgcc
     4081 tggaaaccgg aatacaggga gttggggacg tccgggaatg ccgccgcgac gtccggacgg
     4141 gtcgtcccgt agatcgccgt cgagggcagg ccccgcccga ccgctccggc gacgatctgg
     4201 ccgtccatca ggaaatcgac atggtcgacg tcctcatcgt cgatcgccca gcccgaaacc
     4261 ggcaggggtc catcggtgcc ctcgaccccg ctcggccccg ggatatcgat gaaaccgaag
     4321 ggcgcctggt tgactgtatt gtcgatgatc accgtgatcg ggccgatcag gaaagtcgcc
     4381 tggctcgctt ccgtgacgcg gatcgagagc gcatgcgcgc cgtccgggta tcgtctcgcc
     4441 aggaacgacg taacgaagcc ggggttggga ttggcgctgt tcacgtaggt cggaaagacc
     4501 tcgaggacgt ctgcccgcgg caggttcgtg tcggcgcggt tcgcgaaggt gccgtccacg
     4561 agcagttcga ccctgtcgat ggcgttgaaa tcgagcaccc agccggtgac cttgacgacg
     4621 ccggaaaccc gctggccctg ggtcggctgg tcgatgaccc cgatcggttg agcagagata
     4681 gacggtgcga gtgccccggc tccgacgaac agaagtgcca ggagcccggg cgacttccta
     4741 agcatgcgct ccctcccgaa aatttaagaa aagaatgagc aaaatggggc ggaaagtcaa
     4801 gctccgtcgg aacttcgcct catttttttc ggcgaaactc cggcgaaaga tgccaagctc
     4861 ttcgaccttg acgtctcaag ttgcgccgaa ttccggccct tcccggtcga agcgggcgct
     4921 gaagtcgcgc ccgatccaga tcgggctctc ggtcatcctg gccgcggccc tcctcgtgtt
     4981 cttcctgcgc gggatcaact tccgcgacat cgccaaccag gtcgcggcgg cgtccggcgg
     5041 ctggctcggc gcgagcgtcg tgctcgcgat cgggacgttt ctctttcgcg ccctacggtg
     5101 gacgtggctc ctccggccgg tcgcgcgggt ccgtttctac ccggccttcg tcgcgaccgc
     5161 cttcgggttc gccgcgaaca acctgccggg aaaagtgggt gaggtccttc gccccgccct
     5221 cctggcccgc tcggagaagc tccccttttc gccgctgctc gcctccgtgc tcctcgaacg
     5281 ggttttcgac ggagccgcag ttcttttctt cttcgtcatc gcggcggcga ccgggattcc
     5341 gaacacgcac gggacgggga agctgctcct tcccgccggc ctcctcctgg tcctcgtcgg
     5401 cgcggtcctc ttctcggtgt tttggcgcgt cgagaccgag cgcttcctcg agaaattctg
     5461 gcgccgcctg cccgaacggt ttcagccccg cgcccgcact ttcgcggaga cgttcgtcga
     5521 cgggttcgcc tcgctcaaga atccgaagct gctgctgctc gtaacgttcg ggtcactcgc
     5581 gatgtggttg atcatcaacc tgcagatcta cgcggtgctg cgcgccttcc atctcgacct
     5641 gccgttctcc gcgagcttcg tcgtcaccgc ggccgctgtc ctcggactca tgttcccgac
     5701 tccgggaggt ctcggctcgt atcacctcgc cgtgcagatc gcgctgacgg acgtctacgg
     5761 cgtcgccaag gacacggcat ccggagtcgc tctcctcgcg cacgccatct cgttcgttcc
     5821 gatcaccctc ctcgggctgg cgctcttcgc catgactccc attcgcggga agcagatcga
     5881 ggagctggag cggataaccg agaccggaat ggaaaagcgg gaaccgtaac gcgcaaacgc
     5941 agcgcgtgcc cctttctcgt ttcccgcagt cataatggag cctcatgaag tgtcccttct
     6001 gcggggcagt cgatgaccga gtcgtggatt cgcgcgagag ccgcgaaggc gaggtcatcc
     6061 ggcggcgcag ggaatgtgaa tcttgcgggc ggcggttcac gtcgtatgaa acgatcgagg
     6121 agatcccgta catggtcgtc aagaaggacg gccgccggga acacttcgat cgaaagaaac
     6181 tccgcgccgg gctggtgaag gcttgcgaga agcggccggt gtcacccgcg cagctcgaca
     6241 cgatcgtgga tgagatcgag acgcagcttc acgacaccga ggagcgcgaa atgtcgacga
     6301 gcgacattgg ggcccgggtc atggatcggc tccgcgagct cgacaaggtc gcatacgtcc
     6361 gcttc
//
LOCUS       DASWAV010000212         4354 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U2665
            k141_291092, whole genome shotgun sequence.
ACCESSION   DASWAV010000212 DASWAV010000000
VERSION     DASWAV010000212.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960316
            Sequence Read Archive: SRR10260007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 4354)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4354)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:47:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,929
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4354
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_291092"
                     /isolate="SMAG_U2665"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.36444 N 132.31889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..203)
                     /locus_tag="VF958_04240"
     CDS             complement(<1..203)
                     /locus_tag="VF958_04240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018131696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HEX9148352.1"
                     /translation="MLTYGATPRVDLETWTFRCFGLVENVLSWTWNEFLALPRIEVTS
                     DIHCVTRWSRFDNRWEGVAVSEIL"
     gene            complement(271..909)
                     /locus_tag="VF958_04245"
     CDS             complement(271..909)
                     /locus_tag="VF958_04245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9148353.1"
                     /translation="MLRQAALAISLLAAGTAAAPKLKTTGPLDDKAAPEAVRSALEPG
                     GERVLLPEGQWCEVWFRKKIPEEKNSTPGALQSNLGISTAVGVIRFLDAATDFRGQAI
                     PPGLYTLRYATIPNDGNHLGVSEYPDFLLVVPVADDPDPGARFTLEDLTRLSVKATGT
                     KHPGVLSLTRPSGESFPSVRVDGAGHVVLQMKASIGAVEAPIALVVKGQVPQ"
     gene            complement(941..1624)
                     /locus_tag="VF958_04250"
     CDS             complement(941..1624)
                     /locus_tag="VF958_04250"
                     /inference="COORDINATES: protein motif:HMM:NF018712.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1326 domain-containing protein"
                     /protein_id="HEX9148354.1"
                     /translation="MGNRTMGKKLAFVSALTLLLAGAAAAAQITGDYIETRSADVYTG
                     QCFANGEMNLVGNQALLAWQVRSGTWNGVRLDGLVVAGAVRANATLGDPYANPYPARS
                     VVLVDERATAPQREALVAFAKAMAGELLENVVEVETAPMAMSVEKHGTHATRGFFRAT
                     GLAEIETRAIGDKDHICGNEYTFYPPLTATEHAMPAVALRDEYQGPGLGVSWISREKR
                     SAFVGSFER"
     gene            complement(1669..1905)
                     /locus_tag="VF958_04255"
     CDS             complement(1669..1905)
                     /locus_tag="VF958_04255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9148355.1"
                     /translation="MLAQILERFDTRIPDLCHCLRWKGQFITADRDPTVPPSNDGLFW
                     CSHTQKCIGPDGGLAEPGNCSSPGRGCHEGGHEG"
     gene            complement(1920..2912)
                     /locus_tag="VF958_04260"
     CDS             complement(1920..2912)
                     /locus_tag="VF958_04260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011684180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD binding domain-containing protein"
                     /protein_id="HEX9148356.1"
                     /translation="MRAFEYASARSKQQAAELLGAKWGDAEVLAGGSDLLALMKDDVV
                     RPRRLVDIKEIPEMRGIRAAGAGFSLGALVTLAELQDDSRLRQRYPALAHALDDAASP
                     QIRNVATLGGNLCQRPRCWYFRGGHGLLARGPDGESLVPKGDNRYHAVLGNEGPAYFV
                     SPSTIAPALVAYGAKVRLFGARGERELPLEKFFRTPKSDGEREHDLAPNEIVTEIVLP
                     SAEGTRSASYEVRQKEAFDWPYATASVVIRMSGNTAQSARIVLGHVAPIPWISPEAEA
                     AVAGKPLDETTAAAAGRAAVAAAKPLSRNRHKIQLATIAVKRALLLAARAAVRG"
     gene            complement(2899..>4354)
                     /locus_tag="VF958_04265"
     CDS             complement(2899..>4354)
                     /locus_tag="VF958_04265"
                     /inference="COORDINATES: protein motif:HMM:NF042649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="molybdopterin cofactor-binding domain-containing
                     protein"
                     /protein_id="HEX9148357.1"
                     /translation="VTARLSKKAGGKPVKIMLERSADQQVAGARPSAYARVKVGAKKD
                     GTLLAWESRGWGTGGIGGGGSPPLPYIVNVPNQRKQYIAIATNIGSARAWRAPNHPQA
                     AAITMCALEDLCARLGMDPVDFFLKNIDLTGPRAKTYTEEIHKAAELMDWKRKWHPRG
                     DPAPGPVKRGLGMSIHTWGGRGHASECDFAIYPDGSVVVKLGSQDLGTGTRTCIQIVA
                     AESLGLPFDAVKVEIGDTRYPVSGGSGGSTTIGGVSSSTRRGAIDALNQIFAKVAPAL
                     KAKPEELEAADGAIRVAADPGRRLSWKEACAKLGAMPVTARGVNKSGMTPDLTGSGVG
                     GAQMAEVTVDVETGIVNVEKIVAVQDCGLIIDLKTAESQCYGALIMGIGYSLYEEKVM
                     DETTGRMLNPNMEFYRLAGLGDIPELVVHMMTGPGYDERGVIGLGEPPVVSPGAVLSN
                     AVANAIGVRVPSLPLTPDRVLAALEGKEAQRAGL"
BASE COUNT          661 a         1487 c         1454 g          752 t
ORIGIN      
        1 aggatctccg aaaccgcgac cccctcccac cggttgtcga accggctcca tcgcgtcacg
       61 cagtggatat cggaggtgac ctcgatccgc ggcagcgcga ggaactcgtt ccacgtccac
      121 gaaagtacgt tctcgaccag gccgaagcac cggaaggtcc aggtctccag gtcgacgcgc
      181 ggcgtggcgc cgtacgtgag cacgggccat ttctcggtga ggacctggcc ggaaggcagg
      241 cgcccggatg tcggaatgtc catacggaac ttactgaggg acctgtccct tcacgacgag
      301 cgcgatcggt gcctccacgg cgccgatgct cgctttcatc tgcagcacga catgtccggc
      361 gccgtccacc ctcacggatg gaaaactttc accggaaggc cgggtgaggg agagaacgcc
      421 cggatgcttc gtgcccgtcg ccttcaccga gagcctcgtc aggtcttcga gcgtgaatcg
      481 agcgccggga tcgggatcgt cggcgaccgg gaccacgagg agaaagtccg gatattccga
      541 gacgcccagg tggtttccgt cgtttggaat cgtggcgtag cgaagggtgt agaggcccgg
      601 tgggatcgcc tggccgcgaa agtcggtggc cgcgtccagg aaccggatca ccccgacggc
      661 ggtggagatt ccgaggttcg actgaagcgc tccgggcgtc gagttcttct cttccggaat
      721 cttctttcgg aaccagacct cgcaccactg cccctcgggc agcagaaccc gctcgccccc
      781 gggctcgagc gccgagcgca ccgcctcggg cgctgccttg tcgtcgaggg ggccggtggt
      841 cttgagcttc ggcgctgcgg ccgtgccggc cgcgagcaga gagatcgcga gcgcggcttg
      901 tctcagcatt cagtcgtttc ggacgaccgg aacgcggcga ctaacgttcg aagcttccga
      961 cgaacgcgct gcgcttctcc cgggagatcc acgaaacgcc gaggcccggg ccctggtact
     1021 cgtcccggag cgcgacggcc ggcatggcgt gttccgttgc cgtcaggggc ggatagaacg
     1081 tgtactcgtt gccgcagatg tggtccttgt ccccgatcgc gcgcgtctcg atctcggcga
     1141 gcccagtcgc gcggaagaac cctcgggtcg cgtgcgtgcc gtgcttttcg accgacattg
     1201 ccatcggcgc cgtctccacc tcgaccacgt tctcgagcag ttcccccgcc atggccttcg
     1261 cgaaagccac cagcgcttca cgctgcgggg cggtggcgcg ttcatccacc aggacgacgg
     1321 acctggcggg ataagggttc gcgtacgggt ctcccagagt cgcgttggcc cggaccgccc
     1381 cggcgacgac cagtccgtcg agccggaccc cgttccacgt tccgctcctc acctgccagg
     1441 cgaggagcgc ctggttaccg acgaggttca tctcgccgtt ggcgaaacac tggccggtgt
     1501 agacgtcggc gctgcgggtt tcgatgtagt caccggtgat ctgggcggca gccgcagcgc
     1561 ctgcgagcag gagcgtcagc gctgaaacga aagcgagttt cttccccatc gttctgttcc
     1621 ccatgtcacg aacaacccgg acaagcggcc tttcgattcc ccgagtcctc aaccctcgtg
     1681 acccccttcg tggcagccgc gaccggggga ggaacagttg cccggctcgg ccagaccgcc
     1741 gtcgggtccg atgcacttct gcgtgtggga gcaccagaag aggccgtcgt tggacggagg
     1801 aacggtcggg tcgcgatcgg ccgtgatgaa ctgccccttc cagcgcaggc agtggcagag
     1861 gtcgggaatc cgtgtatcga agcgctccag tatctgggcg agcattcctg gccctccccc
     1921 tatcctcgaa cggcggcgcg cgcggcgagg agcagcgcgc gtttgaccgc gatcgtcgcg
     1981 agctggatct tgtgccggtt gcgggagagc ggctttgccg cggcgactgc cgcgcgaccg
     2041 gcagccgcgg cggtggtttc atcgagcggc ttgccggcaa cggcggcttc ggcctcgggg
     2101 gagatccacg gaatcggagc cacgtgaccg aggacgatgc gcgccgactg cgccgtgttg
     2161 ccactcatcc ggataaccac cgacgcggtg gcgtacggcc agtcgaaggc ttccttctga
     2221 cgaacctcgt agctcgcgct ccgggttccc tccgctgagg gcagaacgat ctccgtcacg
     2281 atctcgttgg gcgcgaggtc gtgctcccgt tcgccgtcgg acttcggagt gcggaagaat
     2341 ttctcgagcg gcaactcgcg ctccccgcgg gctccgaaca accggacctt cgccccgtag
     2401 gcgacgagcg cgggtgcgat ggtcgagggg ctgacgaagt aggccgggcc ttcgttgccg
     2461 aggaccgcgt gataacggtt gtcccctttc ggcacgagcg actcgccatc cggtccccgc
     2521 gccagcaggc cgtgaccacc tcggaagtac cagcagcggg gccgctgaca gagattgccg
     2581 ccgagagtcg cgacgttgcg aatctggggg ctcgccgcgt cgtcgagcgc gtgcgcaagc
     2641 gccggatacc tctgccggag ccgggaatcg tcctggagct ccgcgagcgt gacgagcgcg
     2701 cccagagaaa accctgcgcc ggcggcgcgg atcccgcgca tttccgggat ctccttgatg
     2761 tcgacgaggc gccggggacg gacgacgtcg tccttcatca gcgcgagcag gtcgctacct
     2821 ccagcgagga cctcggcgtc cccccacttc gcgcccaaga gctccgcggc ctgctgcttg
     2881 ctgcgcgcgc tggcgtactc aaaggcccgc acgttgcgcc tccttgcctt cgagagccgc
     2941 cagcacccgg tccggagtga gcggcaggga aggcacgcgc acgccgatgg cgttcgccac
     3001 ggcgttggag aggacggctc ccggcgagac caccggaggc tcacccaatc cgatcacgcc
     3061 gcgctcgtcg tacccgggtc ccgtcatcat gtgcacgacg agttcgggaa tgtcgccgag
     3121 gcccgccagc cgatagaact ccatgttcgg gttgagcatt cggccggtcg tctcgtccat
     3181 gaccttttct tcgtaaagcg agtagccgat acccatgatg agcgcgccgt agcactggct
     3241 ctcggccgtc ttcaggtcga tgatgagtcc gcagtcctgc actgcgacga tcttctcgac
     3301 gttgacgatt cccgtctcca cgtccacggt gacctcggcc atctgagcgc cgccgacgcc
     3361 gctcccggtc aggtccggcg tcatgccgct cttgttgacg cctcgagcgg tcaccggcat
     3421 ggcgcccagt ttcgcgcagg cctccttcca cgagaggcgg cggccggggt cggccgcgac
     3481 gcggatcgcg ccgtccgcgg cttccagctc ttcgggcttc gctttcaacg caggtgccac
     3541 tttcgcgaag atctggttga gcgcatcgat cgcgccgcgc cgcgtcgacg acgagacgcc
     3601 cccgatcgtc gtgctgccgc ccgagccgcc ggagacggga tagcgcgtgt cgccgatctc
     3661 gaccttcacc gcgtcaaacg ggagacccag cgactccgcg gccacgatct ggatgcaggt
     3721 ccgggtgccg gtgccgaggt cctgggagcc gagcttgacc accaccgagc cgtcggggta
     3781 gatcgcgaag tcgcactcgc tcgcgtggcc gcggcccccc caggtgtgga tggacatccc
     3841 gaggccgcgt ttgacgggtc ccggcgcggg atcgccgcgg ggatgccact tcctcttcca
     3901 atccatcagc tcggcggcct tgtggatctc ttcggtgtag gtcttcgccc gcgggccggt
     3961 cagatcgatg ttcttcagga agaaatcgac cgggtccatg ccgagccggg cgcacaggtc
     4021 ttcgagcgcg cacatcgtga tcgccgcggc ctgcgggtgg ttgggcgccc gccacgcccg
     4081 cgcgctcccg atgttcgtgg cgatcgcgat gtactgcttg cgctggttgg ggacgttcac
     4141 gatgtagggc agggggggcg agccgccgcc tccgatgccg cccgttcccc agccgcggga
     4201 ctcccacgcg agcagcgtcc cgtccttttt ggcgccgact ttcactctcg cgtaggcaga
     4261 agggcgcgcg cccgcgacct gctggtcggc cgatcgctcg agcatgatct tgaccggctt
     4321 gcccccagct ttcttcgaga gcctcgcggt cacg
//
LOCUS       DASWAV010000213         5999 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U2665
            k141_650997, whole genome shotgun sequence.
ACCESSION   DASWAV010000213 DASWAV010000000
VERSION     DASWAV010000213.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960316
            Sequence Read Archive: SRR10260007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 5999)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5999)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:47:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,929
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5999
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_650997"
                     /isolate="SMAG_U2665"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.36444 N 132.31889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..746)
                     /locus_tag="VF958_04270"
     CDS             complement(<1..746)
                     /locus_tag="VF958_04270"
                     /inference="COORDINATES: protein motif:HMM:NF013646.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEX9148358.1"
                     /translation="MGENWDAVVVGGGPGGSTVATVLARAGRKVVLLEKEKFPRFHVG
                     ESLLPFNLPIFERLGVGEKVRAAGYQVKHGAHFWNEATGGVRPVDFADGLDDRHPTAF
                     HVKRAEFDHLLLRHAEESGAQVREETSVERVLFEEGRAVGVVARSGSGTEEIRAHAVV
                     DASGQSALLSRQLGRRKFDPVLKRAGLFAHYEGIRWPEGQRPGDILLPVDRSVWYWII
                     PFSDGSCSVGAVFEPSLAAGAASLEEKFDFL"
     gene            779..1531
                     /locus_tag="VF958_04275"
     CDS             779..1531
                     /locus_tag="VF958_04275"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX9148359.1"
                     /translation="MPPVIEFRDVRKAFAPGRPVLDGLSFTVGDGETLALVGPSGCGK
                     TTALKLVNRLLDHDSGDVRVFGRDVRGEDPIALRRQLGYVIQEAGLFPHWTVSRNVEA
                     VPRLLGWRKDAMRARSDQLLELVGLPAAEFGERHPRELSGGQRQRVGVARALAADAPV
                     LLMDEPFGALDPIARRSMQREFLDWKRRLGKAVLLVTHDLREAFRLGDRVAILDRGRA
                     RQIGTPRQILESPADEFVREFVSDELPVRGSL"
     gene            1528..2160
                     /locus_tag="VF958_04280"
     CDS             1528..2160
                     /locus_tag="VF958_04280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015112839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX9148360.1"
                     /translation="MNGLGGYFWERRSEILALTGEHVLLVLASTFLAVAIGVPLGVAL
                     TRSPRLARPVLGFANVVQTIPSLALFGFLIPMPSIGGIGARTAIAALVVYALLPILRN
                     TYTGIRSVDPAVVEAATGIGMTPRQLLRQVELPLALPIVLAGVRIATVVAIGLATIAA
                     AIGAGGLGVLIYRGVAIVDHRLILAGAVPAAVLALIADAGLGFIEKRLGR"
     gene            2157..3026
                     /locus_tag="VF958_04285"
     CDS             2157..3026
                     /locus_tag="VF958_04285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015178888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter
                     substrate-binding protein"
                     /protein_id="HEX9148361.1"
                     /translation="MRRLGVALLVLFAVLGCRQKGERPLRIGSKNFSEQVILGEIAAQ
                     GLEARGVRVDRRLNLGGTFVCHQAITAGELDLYPEYTGTAFTAILKRRPVSDPAFVRV
                     EVARAYGRWKLVWSPSLGFENTFALVVRGGDARRLGLRRISDLAAHPEIRPGFGYEFL
                     EREDGFPGLAKTYGLAFGERPAQMDLGLLYPALETGKVDLVAGNSTDGLIDAIGGVVL
                     EDDKRYFPPYEAAFVVREEVWKEPKVREFLELLGGSIDAAAMRKLNAAVDKDKRRPED
                     VARDFLARLPARR"
     gene            complement(3023..3574)
                     /locus_tag="VF958_04290"
     CDS             complement(3023..3574)
                     /locus_tag="VF958_04290"
                     /inference="COORDINATES: protein motif:HMM:NF025049.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HEX9148362.1"
                     /translation="MAHLPRRDFFGATARAGLALGLAAAGQPLVALAPGKLSKGGDIE
                     LLNTALALEHEAIWAYGLAGGSGLLSEKAKEVGLLFQGSHEIHRDLIVDAVKRKGGNP
                     VEPKKEYVFGVPLANEKDVLELAFKLEVGAARAYLTVVDKFQDRALAASAGKILSDEV
                     LHAAVLRSVLGRDVVPTFRLIEN"
     gene            complement(3679..5163)
                     /locus_tag="VF958_04295"
     CDS             complement(3679..5163)
                     /locus_tag="VF958_04295"
                     /inference="COORDINATES: protein motif:HMM:NF025585.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4331 domain-containing protein"
                     /protein_id="HEX9148363.1"
                     /translation="MKRNPVSRKYAAGIAALCLVFFAAIAPPEASASSHREAPLISSD
                     PEADNTDTYFFLDPVDGGRAILLGNWIPLEEPAGGPNFSHFGEKIRYEFNVDWDGDAI
                     EDVVYRIQFTRHVRNPDTWLQNTGPVNAPNDPNQNVYYTYKVEKILGPSPNQGAAAKI
                     ELGNELLEAPNNVGSKSFPNGYGKGSRIADTVYTIDQDTKVYAGPRADGFYADLGMIF
                     DLINFRPGTLPGNVGGGKNDLAGFNTHTIALSVPVYQLTKNHTVPTDASDPNAIISMW
                     SSTWRLKTRVLSETGDKPSESGDWVQVSRLAVPLVNEVVVPLGQKDLFNASFPRDDAQ
                     FALSVLDPEVPKILKALFNIDSPPAPRNDLLAVVLGVDGLTRRPGEVVSDQLRLNVAV
                     FPTPPSLVNRLGVLGGDLAGFPNGRRPYDDVVDIELRILAGVLVPEFNHSPNNALGDG
                     VDGPDKTYNPAFPFLATPHSGFDHRHDAAAQVTHFSDDPQTTRE"
     gene            5271..5867
                     /locus_tag="VF958_04300"
     CDS             5271..5867
                     /locus_tag="VF958_04300"
                     /inference="COORDINATES: protein motif:HMM:NF019265.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEX9148364.1"
                     /translation="MSSRRDPENRDRSDAALVAAMESRDADALASLYDRHVSRLLGLA
                     YRILGETGEAEEVVQEVFLHAWRAAASFDPARGNVLAWLLVATRSRAIDRIRTRRPIA
                     AVEPSGRDPLDDLPASEDVEEASASREWETACRGAIRELPSDQRQVLELAYFDGLTHQ
                     EIAERTAMPLGTVKTRIRLGLMKLRERMGPYWTRERNA"
BASE COUNT          923 a         1990 c         2057 g         1029 t
ORIGIN      
        1 agaaagtcga acttctcctc cagcgaggcg gccccggccg ccagagacgg ctcgaaaacc
       61 gctccgacgc tgcaggagcc gtcggagaac gggatgatcc agtaccagac ggagcggtcg
      121 acgggaagaa ggatgtcgcc gggccgctgc ccctcgggcc accgtatccc ctcgtagtgc
      181 gcgaacaacc ccgcccgctt cagcaccggg tcgaacttcc gcctcccgag ctgccgggag
      241 agcagagcgc tctgcccgga ggcgtcgacg accgcgtgcg cgcggatctc ctcggttccg
      301 ctacccgatc gcgcgacgac tcccacggcg cgaccttcct cgaagagaac ccgttccacc
      361 gacgtctctt cgcgaacctg cgcgcccgac tcctcggcgt ggcggagcag gaggtggtcg
      421 aattccgcgc gcttcacgtg aaacgcggtc gggtgtcggt cgtcgagccc atccgcgaaa
      481 tcgaccggcc ggaccccgcc cgtcgcctcg ttccagaagt gggccccgtg cttgacctga
      541 tagcccgcgg cgcgcacctt ttcgccgacg ccgaggcgct cgaaaatcgg gaggttgaac
      601 gggagaagtg attcgcccac gtggaaacgc ggaaacttct ccttctcgag caagacgact
      661 ttccgccccg ctcgcgcgag aacggttgcg acggtcgagc ctcccggccc tccgcccacg
      721 acgaccgcgt cccagttttc gcccatcgct cgattctctc atcacataat tggacgcgtt
      781 gccccccgtc atcgagttcc gcgacgtgcg caaagcgttc gcgcccggac gacccgtcct
      841 cgacggactc tcgttcacag tcggagacgg cgagacgctg gcgctcgtcg gaccgtccgg
      901 ctgcggcaag acgaccgcgc tgaagctcgt caatcggctg ctcgatcacg actccggcga
      961 cgtgcgcgtc ttcggacgcg acgtccgcgg ggaggatccg atcgcgttgc ggagacagct
     1021 cgggtacgta atccaggaag cgggtctttt tcctcactgg acggtgagtc gcaacgtcga
     1081 ggcggtgccg cgccttctcg gctggcgcaa ggacgcgatg agagcgcgtt ccgaccagct
     1141 gctcgagctc gtgggtcttc cggccgcgga gttcggcgag cggcatccgc gcgagctttc
     1201 cggcggacag cgccagcggg tcggcgtggc gcgagctctc gcggcggacg cgccggtcct
     1261 tctcatggac gagcccttcg gcgcgctcga cccgatcgcg cgccgctcga tgcagcggga
     1321 gttcctcgat tggaagcgcc gtctcggaaa ggccgtgctc ctggtgaccc acgacctgcg
     1381 cgaggccttc cggctgggcg accgcgtcgc catcctcgac cgcggacgcg cgcgccagat
     1441 cgggacgccc cggcagattc tcgaatcgcc ggcggacgaa ttcgtgcgcg aattcgtgtc
     1501 ggacgagctg cccgtccgag gttctctttg aacggactgg gcggctactt ctgggagcgg
     1561 cgctccgaga tcctggcgct cacgggagag cacgtcctgc tcgtcctcgc ttcgacgttt
     1621 ctcgcggtcg cgatcggagt tcctctcggc gtcgcgctca cgcggagtcc ccggctcgcg
     1681 cggccggtgc tcggattcgc caacgtcgtc cagaccatcc cgagcctggc gctgttcggg
     1741 ttcttgatcc cgatgccctc gatcggcggc atcggggcgc gtacggcgat cgccgccctc
     1801 gtcgtctacg cgctcctgcc gatcctgaga aatacgtaca cgggaatccg ctccgtcgat
     1861 ccggcggtcg ttgaggccgc gacgggcatc ggaatgactc cgcgccagct ccttcgtcag
     1921 gtcgagctgc cgctcgcgct gccgatcgtt ctcgcgggcg tgcgaatcgc gacggtggtc
     1981 gcgatcgggc tcgccaccat cgccgccgcc atcggcgccg gaggcctcgg ggtcctgatc
     2041 taccggggcg tcgcgatcgt ggaccaccgg ctgatcctgg cgggtgccgt gcccgcggcc
     2101 gttctcgcgc tcatcgcgga cgccggtctc ggcttcatcg aaaagcgcct gggccgatga
     2161 gacgcctcgg tgtcgctctg ctcgttctct tcgccgtcct cgggtgccgg cagaaggggg
     2221 agcgtcctct gcgcatcggc tcgaagaatt tctcggagca ggtgatcctg ggagagatcg
     2281 cggcgcaggg tctcgaggcc cgcggagttc gagtggaccg gcgcttgaac ctcggaggca
     2341 cgttcgtctg ccaccaggcc atcacggcgg gcgagctcga tctctacccg gagtacacgg
     2401 gaacggcgtt cacggcgatc ctgaagagac gtccggtgtc cgatcccgca ttcgtcaggg
     2461 ttgaagtggc ccgcgcgtac ggcaggtgga aactcgtctg gtccccgtct ctcggtttcg
     2521 aaaacacgtt cgcgctcgtc gtgcgcggcg gcgacgcgcg ccgtctgggc ctccggagga
     2581 tttccgatct cgccgcgcac ccggagatcc gccccggctt cggctacgag ttcctcgaac
     2641 gcgaagacgg cttccccggc ctcgcgaaga cctatggtct cgccttcgga gagcgccctg
     2701 cgcagatgga cctggggctt ctctacccgg cgctcgagac cggaaaggtc gacctggtcg
     2761 ccggaaactc gaccgacggc ctgatcgacg cgatcggagg cgtcgtgctc gaggacgaca
     2821 agcgttactt ccccccctac gaagccgcgt tcgtcgtccg cgaggaggtg tggaaagagc
     2881 cgaaggtgcg cgagttcctg gagctgctcg gcggctcaat cgacgccgcg gcgatgcgga
     2941 agctgaacgc tgccgtggac aaggacaagc gccgcccgga ggacgtggcc cgcgacttcc
     3001 tggcgcgact tcccgcgcgc cgctagttct cgatcagccg gaacgtcggg acgacgtcgc
     3061 gccccaggac gctgcgcaga accgccgcgt gcaggacctc atcggaaagg atctttcccg
     3121 ccgaggcagc gagcgcccgg tcctggaact tgtcgaccac ggtcagatac gcgcgcgccg
     3181 cgccgacctc gagcttgaag gcgagctcca acacgtcctt ctcgttggcg aggggtactc
     3241 cgaatacgta ctccttcttc ggctcgacgg ggttccctcc cttgcgcttg accgcgtcga
     3301 cgatgaggtc gcggtggatc tcgtggcttc cctggaagag aagaccgacc tccttcgcct
     3361 tctccgacag caacccgctc cctccggcga gcccgtaggc ccagatcgcc tcgtgctcga
     3421 gcgcgagcgc cgtgttcagg agctcgatgt cgccaccttt cgagagcttt cccggcgcga
     3481 gcgcgacgag cggctgtccg gcggcggcga gtccgagcgc aaggcccgcg cgcgcggtcg
     3541 cgccgaagaa atcgcggcgg ggcagatgag ccatggattg cctcctcctg aagactgaac
     3601 ggtgaaagaa gcctacgtcc aaaagccccg gcccggcttt tcggccggac cagggaccag
     3661 gtggaaacgg ggtccgatct attcgcgggt cgtctgcgga tcatccgaga agtgcgtcac
     3721 ctgagccgcc gcatcgtggc gatggtcgaa tccggagtgt ggcgtcgcga ggaaggggaa
     3781 cgcggggttg taggtcttgt ccggtccgtc gacgccgtcg ccgagcgcgt tgttcggtga
     3841 gtgattgaac tcgggcacga gtactcccgc gaggatccgg agttcgatgt ccacgacgtc
     3901 gtcgtagggg cgccgtccgt tcggaaagcc ggcgaggtca ccgccgagaa cgccgagccg
     3961 gttgacgagc gagggcgggg tcgggaagac ggcgacgttc agccggagtt ggtcggatac
     4021 tacctcgccg ggacgccgcg tgagaccgtc gacgccgagg acgaccgcga ggaggtcgtt
     4081 gcgaggagcc ggaggggagt cgatgttgaa gagggccttc aggatcttcg ggacctcggg
     4141 gtccagcacc gaaagcgcga actgcgcgtc gtccctgggg aacgatgcgt tgaagaggtc
     4201 cttctgcccg agcggaacga ccacctcgtt gacgagaggg accgcgagcc gtgagacctg
     4261 gacccagtcg ccggactccg acggcttgtc gccggtctcg gagagcacgc gagtcttcag
     4321 gcgccacgtc gaggaccaca tgctgatgat cgcgttgggg tcggaggcgt ccgtcggaac
     4381 ggtgtggttc ttcgtgagtt gatagaccgg gaccgagagg gcgatcgtgt gggtgttgaa
     4441 cccggcgagg tcgttcttgc cgccgccgac gttgccgggg agagtgcccg gccggaagtt
     4501 gatcaggtcg aagatcattc cgaggtccgc gtaaaagccg tcggcgcgcg gtcccgcgta
     4561 aaccttcgta tcctgatcga tcgtgtacac cgtatccgcg atgcgcgagc ccttgccgta
     4621 tccattgggg aagctcttcg aacccacgtt gttcggcgcc tcgagcagtt cgttaccgag
     4681 ctcgatcttt gccgcggcgc cctggttcgg cgaggggccc aggatctttt cgaccttgta
     4741 ggtgtagtag acgttctggt tcgggtcgtt cggcgcgttc accgggccgg tgttctggag
     4801 ccacgtgtcg gggttgcgca cgtgacgcgt gaactggatg cggtagacga cgtcctcgat
     4861 ggcgtcgccg tcccagtcca cgttgaactc gtagcggatt ttttccccga agtgggagaa
     4921 gttcggcccg cccgccggct cttccagcgg gatccagttg ccgagcagga tcgccctccc
     4981 gccgtcgacc ggatccagga aaaagtaggt gtctgtgttg tccgcctcgg gatcgctgct
     5041 gatcaacggc gcctcgcggt ggctcgaagc gctcgcttcc ggcggagcga tagcggcgaa
     5101 gaacacgaga cagagcgcgg cgatcccggc cgcgtatttt cgggataccg ggttccgttt
     5161 catcgaggtt cctccttcct tgtggtctcg ggttcctcta cgaggcgggg gggccggttg
     5221 gatttgcggc gccgcggccg ggttatcctt ccgcgccgac cagaaatcca atgagctccc
     5281 gccgggatcc ggaaaatcgg gatcggagcg acgcggcgct cgtcgccgcg atggagagcc
     5341 gcgacgcgga cgcgctcgca tcgctgtacg accgccacgt gtcgcggctt ctcggcctcg
     5401 cataccggat cctcggagag acgggagagg ccgaggaagt cgtgcaggag gtcttcctgc
     5461 acgcgtggcg cgccgccgcg agcttcgatc ccgcgcgcgg caacgtcctc gcgtggctcc
     5521 tcgtcgcgac ccgcagccgc gcgatcgacc ggatccggac ccgccggccg atcgcggccg
     5581 tcgagccatc gggacgggat ccactggacg atctcccggc ttcggaggac gtcgaggaag
     5641 cgtccgcgtc gcgcgaatgg gagacggcct gccggggggc gatccgcgag ctgccctccg
     5701 accagcgcca agtcctcgag ctcgcctatt tcgacggcct gacccaccag gagatcgcgg
     5761 agcggaccgc aatgcccctc gggaccgtca agacacgcat ccgcctcggg ttgatgaaac
     5821 tccgtgagcg catgggtccg tactggacga gggaacggaa tgcatagcga gaggcacgaa
     5881 gagctgctct ctctggcggc gctcggcgtg ccgctcgggg gcgatgatgc tgccgagtac
     5941 gcgcggctgc tcgaggaggc ctgcccggag tgcgcggcgc ttctgcccga actgcgggc
//
LOCUS       DASWAV010000214         3128 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U2665
            k141_531286, whole genome shotgun sequence.
ACCESSION   DASWAV010000214 DASWAV010000000
VERSION     DASWAV010000214.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960316
            Sequence Read Archive: SRR10260007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 3128)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3128)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:47:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,929
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3128
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_531286"
                     /isolate="SMAG_U2665"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.36444 N 132.31889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..288
                     /locus_tag="VF958_04305"
     CDS             <1..288
                     /locus_tag="VF958_04305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9148365.1"
                     /translation="PVSVEDVRRTVTAYGFRFPVAVDDDWKALRRLWLDRSPGAGWTS
                     AALVIDRDGIVRHVHPGGVFAKDSADPQARRDYDEIRATIETLLARKEVPR"
     gene            285..1040
                     /locus_tag="VF958_04310"
     CDS             285..1040
                     /locus_tag="VF958_04310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9148366.1"
                     /translation="MRLRLPAILFLAIATCGAGGSVRHDADALVALGAGISGTWETEF
                     DFANPDSNNAVNVFVGEQPFPFVCVAAPCGIEYAPLPRNGTARIATRSEDFAGFTGRL
                     FVGADDAEELPTVTARVVNRARPSQTIELPLYRRSTLVALNPLSLSFPGAERTSTSHA
                     NLFVSEIGLSWEVAVLVEAYASAGERLGGEVHVIPRGETLVLQDVLPRLGVTGLSQGQ
                     LRLTRTAGNGVMWGVLATVFDDGRVSVTAGKHP"
     gene            complement(1172..1681)
                     /locus_tag="VF958_04315"
     CDS             complement(1172..1681)
                     /locus_tag="VF958_04315"
                     /inference="COORDINATES: protein motif:HMM:NF024273.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HEX9148367.1"
                     /translation="MTDDSRADRAAHARVVQRLRDQADDVRRLVAGLDEESLSKRTIP
                     EKWSLKELVCHVLKVQRVFSERLERMLAEESPSVTSYKAENDPGFEKMTARPAEETLS
                     AYLAERERHLERLEDLSPAQWHRVAQHPDFPIYDVHFQTDYLAHHEAHHIFQMLQRRT
                     PFGRIPPEG"
     gene            complement(1711..>3128)
                     /locus_tag="VF958_04320"
     CDS             complement(1711..>3128)
                     /locus_tag="VF958_04320"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011141702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HEX9148368.1"
                     /translation="RIYFTSDRVDEPYYSDPDANVYFVPAAGGEIQKVVDIDGPIDDP
                     EPSPDGRRFAFTGFLNPSQRQSHTNAELFVFADGKATNLTSDYDYEPGSDLAGDQRPP
                     RGGLPTPIVWTPDGRSLLLVTTEKGRSNLVKVDVASRRIDPLTTGDHDVMGYTATPDA
                     SKLAVAIGDATHIGDLYLLDAKTKRLTQLTRVNDGLFSQLQLPQIEEIWYPSFDGQRI
                     NGWIVKPVGFEAGKKYPLILEIHGGPHTAYGHILYHEMLWMASKGYVVLYVNPRGSTS
                     YGQDFANVIQYRYPGDDYKDIMAGVDEVLKRGYVDEKRMGVTGGSGGGLLTNWTVTQT
                     DRFAAAVSQRSVADWYAFWNMADFTLFTPSWFRKNPYEDPEEFLSRSPVRYAEKIKTP
                     MMFIEGEEDYRTPPGQGGEAMFRALKAQKKTAVMVNFPGENHELSRSGKPSHRVERLQ
                     HIVNWFDKYLQGKEIHLYDLQ"
BASE COUNT          473 a         1025 c         1031 g          599 t
ORIGIN      
        1 cccgtgtccg tcgaggacgt tcgccggacg gtcacggcat acggcttccg cttcccggtc
       61 gcggtggacg acgactggaa ggctctccgc cgtctctggc tcgaccgctc tccgggcgcc
      121 ggctggacgt ccgcggccct cgtcatcgat cgcgacggta tcgttcgcca cgtccatccc
      181 ggaggcgtgt tcgcgaagga ctctgccgac ccgcaggctc gccgcgacta cgacgagata
      241 cgcgcaacga tcgaaacgct tctcgcccgc aaggaggtcc cccgatgaga ctccgcctgc
      301 cggccatcct cttcctggcg atcgcgactt gcggcgcagg tggctccgtg cgacacgacg
      361 cggatgccct cgtcgccctc ggagcgggga tttccggaac gtgggagacc gagttcgatt
      421 tcgcgaaccc ggactcgaac aacgccgtga acgtcttcgt gggagaacag ccgttcccat
      481 ttgtctgcgt ggccgcgccg tgcgggatcg aatacgcacc tctcccgcgc aacggaaccg
      541 cacggatcgc gacccgcagc gaggacttcg ccggctttac cggccggctc ttcgtcggcg
      601 cggacgacgc cgaggagctg ccgacggtca cagcgcgggt ggtcaaccgg gcccgccctt
      661 cacagacgat cgagcttccg ctctatcgcc gctcgaccct cgtcgcgctc aacccgctct
      721 ccctgtcgtt tccgggcgcg gagcggacgt cgacgtcgca tgccaacctg ttcgtctcgg
      781 aaatcgggct ctcgtgggaa gtggcggttc tcgtcgaggc gtatgcgtcg gcgggcgagc
      841 ggctgggcgg agaagtccac gtcattccgc ggggtgagac gctcgttctt caggacgtcc
      901 ttccgcgcct gggcgtcacc gggctctcgc agggccagct tcggctgacg aggaccgcgg
      961 gtaacggcgt gatgtggggg gtgctcgcga ccgtgttcga cgacggacgc gtcagcgtca
     1021 ccgccgggaa gcacccctaa aagggggtca ggtctccact tgccacattg ggggtcaggt
     1081 ctctacttgc cacatcgcta cgcgatgatg tcaagggcgc tctatcgttt cgagcgcttt
     1141 ccgtatctgt gaagacctga ccccgctttt ctcacccctc gggcgggatc ctcccgaacg
     1201 gcgtccggcg ctgcagcatc tggaagatgt gatgcgcctc gtggtgcgcg aggtagtcgg
     1261 tctggaagtg aacgtcgtaa atcggaaagt cggggtgctg cgcgacccgg tgccattgcg
     1321 cgggcgacag gtcttcgaga cgctccaggt gccgctcgcg ctccgcgagg taggcagaga
     1381 gcgtttcctc ggccgggcgc gcggtcatct tctcgaaacc ggggtcgttc tccgccttgt
     1441 aagaggtcac cgacggactc tcttccgcga gcattctctc gaggcgctcc gagaacaccc
     1501 gctgaacctt cagcacgtgg cagacgagct ccttcagcga ccacttctcg gggatcgtgc
     1561 gcttcgagag ggactcctcg tcgaggcccg cgacgagacg gcgtacgtcg tcggcctggt
     1621 cgcgcaggcg ctggacgacc cgagcgtgtg cggcgcgatc ggcgcgggag tcgtcggtca
     1681 tgacgaaatc atacgttggt ctcggacgag ctactgcagg tcgtaaaggt gaatttcctt
     1741 cccctgcagg tacttgtcga accagttcac gatgtgctgg agccgctcga cgcggtgcga
     1801 cggtttcccc gagcgagaga gctcgtggtt ctcgcccggg aaattcacca tcaccgccgt
     1861 cttcttctgc gccttcagcg cgcggaacat cgcctcgccc ccctggccgg gcggcgtgcg
     1921 gtagtcctct tccccctcga tgaacatcat gggcgtcttg atcttctcgg cgtaccgcac
     1981 gggcgagcgc gacaggaact cttcgggatc ctcgtacggg ttcttgcgga accagctcgg
     2041 ggtgaagagc gtgaagtccg ccatgttcca gaaggcgtac cagtcggcga ccgagcgctg
     2101 tgacaccgcc gccgcgaaac ggtccgtctg cgtgacggtc cagttcgtca gcagtccccc
     2161 gccgctgcca cccgtgaccc ccatccgctt ctcgtcgaca taaccgcgct tcagcacctc
     2221 gtcgacgccc gccatgatgt ccttgtagtc atcgcccggg tacctgtact ggatgacgtt
     2281 ggcgaagtcc tgaccgtagg acgtcgagcc gcgcgggttg acgtagagga cgacgtagcc
     2341 cttcgacgcc atccacaaca tctcgtggta gaggatgtgg ccgtaggccg tgtgcggtcc
     2401 gccgtggatc tcgaggatca gcgggtattt ctttccggcc tcgaagccca ccggtttgac
     2461 gatccagccg ttgatcctct ggccgtcgaa gctcgggtac cagatttcct cgatctgcgg
     2521 cagctggagc tgcgagaaca ggccgtcgtt cacgcgggtg agctgcgtga gtcttttcgt
     2581 cttcgcgtcg aggaggtaga ggtcgccgat gtgggtcgcg tcgccgatcg cgactgcgag
     2641 cttcgacgcg tccggcgtcg ccgtgtagcc catgacgtcg tggtcgccgg tcgtcagcgg
     2701 gtcgatcctt cgcgacgcga cgtccacctt caccaggttt gaacgcccct tctcggtcgt
     2761 cacgaggagg agcgagcggc cgtccggcgt ccacacgatc ggcgttggca gaccgccgcg
     2821 cggcggccgc tggtcgccgg cgaggtcgct ccccggctcg tagtcgtagt cggacgtaag
     2881 gttcgttgct ttgccatcgg caaagacgaa gagttccgcg ttggtgtgcg actgccgctg
     2941 cgatgggttg aggaagcccg tgaaggcgaa acgcctgccg tcgggcgacg gctccgggtc
     3001 gtcgatcgga ccgtcgatgt cgacgacctt ctggatttcg ccgccggccg ccgggacgaa
     3061 atagacgttc gcgtcgggat cgctgtagta cggctcgtcc acccggtcgg acgtgaagta
     3121 gatccgcg
//
LOCUS       DASWAV010000215         5562 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U2665
            k141_330538, whole genome shotgun sequence.
ACCESSION   DASWAV010000215 DASWAV010000000
VERSION     DASWAV010000215.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960316
            Sequence Read Archive: SRR10260007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 5562)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5562)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:47:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,929
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5562
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_330538"
                     /isolate="SMAG_U2665"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.36444 N 132.31889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..425)
                     /locus_tag="VF958_04325"
     CDS             complement(<1..425)
                     /locus_tag="VF958_04325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9148369.1"
                     /translation="MPRGSNIARVIALMALGAGAAVLSNLTASTQRKLAWAGSYPRAL
                     DAEAPAVEPAPTASIPAPAEPTAPPAVSAGSPPAVQVTAPPAPPAALEPDLAKSFPPH
                     PDKSAIEVTPENVLLLHRRNALLMFPVLTVRANKAAGDVP"
     gene            complement(459..788)
                     /locus_tag="VF958_04330"
     CDS             complement(459..788)
                     /locus_tag="VF958_04330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9148370.1"
                     /translation="MSRASREANPGRSVEDSYFGEDPLQSFRRLALPAQSLPCEPDPL
                     LCRHLRGKAWSVVHPAVDPADPTEPFLCLKTLRRRGPDGARVLPDACTDERGCFEAAN
                     CSCGGDS"
     gene            complement(843..1472)
                     /locus_tag="VF958_04335"
     CDS             complement(843..1472)
                     /locus_tag="VF958_04335"
                     /inference="COORDINATES: protein motif:HMM:NF024214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HEX9148371.1"
                     /translation="MKLGAVVPAAGRSLRMGSEKVLLPFAGSTMLGTVLSKLSEAGVA
                     RSVVVLRPDSAEAHLIARAAGAEIVINPHPEDEMLVSIRLGIEPLEGAVDVFFVWPAD
                     HPAVSTATLRELASRASRDLAVIPVHSGRRGHPAIVGTDLLADMARIRPSAGLRQLWR
                     DRADRVRELEVDDPGILENLDDPETYERVRRRAERELRERGGDFAGKSE"
     gene            complement(1469..1996)
                     /locus_tag="VF958_04340"
     CDS             complement(1469..1996)
                     /locus_tag="VF958_04340"
                     /inference="COORDINATES: protein motif:HMM:NF025908.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HEX9148372.1"
                     /translation="MSLPHVLLGLLDGEPRTGYDLASAIREEVEPVWKAEISQIYPAL
                     ARLRREGFVVLRVLGPRRGPHRNLYRVTAAGRRELRRWLLEPPPPPRARDDGLARIAL
                     LDSLGVPERRAVLRAYERALTDEIARLASAPPPEGFRREAREGAVAKLEGARRWARSL
                     AARPSLAGALPGKKK"
     gene            2474..3310
                     /locus_tag="VF958_04345"
     CDS             2474..3310
                     /locus_tag="VF958_04345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006928295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MqnA/MqnD/SBP family protein"
                     /protein_id="HEX9148373.1"
                     /translation="MTPITLAHSPDSDDAFMFYGLARGRVDTEGLAFSHELSDIESLN
                     RRAMAGELDVTAVSFHAYAYLADRYLLLPHGASFGDGYGPVVVARQAMSREEIASGKV
                     IVASPGRWTTAALALALWAPGVPTKEVSFDRIQPDVASGRFDAGVLIHEGQLTYSEEG
                     LTGVVDLGWWWKSETGLPLPLGGNVVRRDLGEDRIARISRSLRRSIEYGLAHREDALS
                     HALAYARGLDRTRADRFVGMYVNEWTRDYGVEGRRAVQTLLDRGAAAGIIPRVRAEYA
                     AD"
     gene            3297..3857
                     /locus_tag="VF958_04350"
     CDS             3297..3857
                     /locus_tag="VF958_04350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9148374.1"
                     /translation="MRLTRLQAPILFLHLAAAGLALGPALFFGAVVAPATFRVLPTRD
                     LAASLTSPVLSGACLLAEVAFGVLFITGWWLTRRDVPRVTRSLLTRLPVAGFFAALAI
                     RELLIPPMDKIRAEAPGLIDNLPAADPSRILLDRYHRLATGFFGAMIAVAVAVLFLTA
                     RFLSIGGNGQPPAASRPPVPKLLDLS"
     gene            complement(3881..4381)
                     /locus_tag="VF958_04355"
     CDS             complement(3881..4381)
                     /locus_tag="VF958_04355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9148375.1"
                     /translation="MKDRRSLTLGVILLVLGGYFLLSRTFRFSGPGALLVLLGTVFLA
                     LSAIRGFRGPVLPGGILLGLGAGFLLRAPLEWLLPAWATIVLGLGAGFLLVAFLDRVA
                     GRGARRPSPLVPGVILIAVALFGVLARHTPILDVLGQVQNLWPWALVAAGVLLVGQAL
                     IRRRSA"
     gene            complement(4378..5250)
                     /locus_tag="VF958_04360"
     CDS             complement(4378..5250)
                     /locus_tag="VF958_04360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004216071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase family protein"
                     /protein_id="HEX9148376.1"
                     /translation="MKILVVGAGVMGRGIAGLCALSGHETLVFDADPEAQRHVRGAIE
                     SSWARAAERGKVAAEAVLRGRRLLKIVERLRNAAGVDFAIEAVPEAIDVKQMVMSELD
                     DLCQDRAILASNTSSLSITRIGRATGRRDRVIGIHFFNPVAAMPLVELVRGEDTSEET
                     MDRALLLVQSLGKEAIRVKDSPGFATSRLGVALGLEAIRMVEEGVALPEDIDRAMELG
                     YGHRMGPLKTSDLVGLDVRLAIAEGLASELSGERFTPPPLLRKMVAEGRTGRKSGEGF
                     YRWEGDKPHPRGRA"
     gene            complement(5313..>5562)
                     /locus_tag="VF958_04365"
     CDS             complement(5313..>5562)
                     /locus_tag="VF958_04365"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX9148377.1"
                     /translation="VFLARRHRNVHLDLSGIPPRKLLEYIPRLEEVSDRCLWGTDFPS
                     PGIRSMRANVEDFLALPLSDDAKRKILFDNAEELLGNR"
BASE COUNT          891 a         1869 c         1912 g          890 t
ORIGIN      
        1 ggcacatcac ctgcagcttt gttggccctg acggtgagta cggggaacat caggagcgcg
       61 tttcgccggt ggaggagcag gacgttctcg ggcgtgacct cgatcgccga cttgtcgggg
      121 tgtggcggaa acgacttcgc gaggtcgggt tccaacgcgg cgggaggagc cggcggagcg
      181 gtcacctgga ccgccggtgg agatccggcc gacacggccg ggggcgcggt cggctccgcc
      241 ggtgccggaa tcgacgccgt cggcgcgggt tccacggcgg gagcttcggc gtcgagcgcc
      301 cgcggatagc tgccggccca ggcgagcttc ctctgggtcg aggctgtcag gttcgagagc
      361 accgcggccc ccgccccgag ggccatgaga gcaatgacgc gcgcgatgtt cgagccgcgt
      421 ggcatgagag ggggattatc gcccggagat cctccggatc aggaatcccc gccgcaggag
      481 cagttcgccg cttcgaagca gccgcgctcg tcggtgcagg cgtcgggaag cacccgggcg
      541 ccgtcgggcc cccgacggcg gagcgtcttc aggcagagga acggctcggt cggatccgcc
      601 gggtcgactg cggggtgaac gacggaccac gccttccccc gcagatgccg acagagaaga
      661 gggtcgggct cgcacgggag agattgagcg ggcagcgcga ggcgacggaa ggactgaagg
      721 gggtcttctc cgaagtacga gtcctcgacc gaacggccgg ggttcgcctc gcggctcgct
      781 ctgctcaagc ggctgcctga atcgctccga tcagttacga ctccgagaag ttgggaaaaa
      841 tcttactccg attttcccgc gaagtctccg ccgcgttccc ggagctccct ttctgcgcgc
      901 cggcggacac gctcgtaggt ctctgggtca tcgaggttct cgaggatgcc gggatcatcg
      961 acctcgagtt cgcgcacccg atcggcgcgg tcgcgccaga gctgccgaag accggcgctc
     1021 ggtcgaatcc gcgccatgtc cgccaagagg tcggttccca cgatcgccgg atgcccccgc
     1081 ctgccggagt ggaccggaat gactgcgaga tcccgggagg cgcggctcgc gagctcgcgg
     1141 agcgtcgccg tggagacggc gggatggtcg gccggccaca cgaagaagac gtcgaccgct
     1201 ccttccagag gttcgatccc gagacggatc gaaacgagca tttcgtcttc ggggtgcgga
     1261 ttgatgacga tttcggctcc ggcggcccgc gcgatgaggt gcgcctcggc ggaatccggc
     1321 cgcagtacga cgaccgatct cgcgacaccc gcttccgaga gcttcgagag aaccgtgccg
     1381 agcatcgtcg atcccgcgaa gggcaggagg accttttcgc ttcccatccg aagagatcgg
     1441 cccgcggccg gcacgaccgc tccgagcttc acttcttctt gcccggaagg gcgccggcga
     1501 gcgaggggcg tgccgcgaga gagcgcgccc atcgccgggc gccctcgagc ttcgcgaccg
     1561 cgccctcccg cgcctcccgc cggaaccctt cgggcggggg ggcggaggcg agacgcgcga
     1621 tctcgtcggt gagggccctc tcgtaggcgc gcaggacggc gcgccgctcc gggacaccga
     1681 gcgaatcgag gagggcgatg cgggcgaggc cgtcgtcgcg ggcccggggc ggcggaggcg
     1741 gctcgaggag ccagcggcgc agctcccggc ggccggcggc ggtcacccgg tagaggttgc
     1801 ggtgaggtcc gcggcggggc cccaggaccc gaagaacgac gaaaccctcc ctccggagac
     1861 gggcgagcgc ggggtagatc tgggagatct ccgccttcca gacgggctcc acctcctcgc
     1921 ggatcgcact ggcgaggtcg tagccggtgc gcggttcacc gtccaggagg ccgagaagga
     1981 cgtgcggaag agacaacgga aaactatatc aagattgata tagcaggcgc tttgcggcgg
     2041 cccgaaactg tccggaaaag agaaaagtca aggcaaaaaa aagggggccc gcaggccccc
     2101 acagaagtct cgtgggtttt gctaagtcct tgagttactt cttcttcttg ccgcctttct
     2161 tcgtggcttt cttggcggcc ttcttcttcg tggccatgac gtcacctccc tcccgacagc
     2221 tgattgacag cctttgggcg aggtgctaat tttgccgccc ccttggaggg gcgtgtcgcc
     2281 ttcagtctgt cgctgccgaa cgattgacag gtcatacgtc ctgatcgccg gatgggccgc
     2341 aacccctccg gcgattcaat tttaggttct tcttcttcgg atacaacaat tatttctgct
     2401 tcgtggggtt caagttgccg gcattctcct gctcatgcga gtgcgatgtc aagcgcgcga
     2461 ctagaatccg cgcgtgactc cgatcaccct cgcgcacagc ccggactccg acgacgcctt
     2521 catgttctac ggcctcgccc gcgggcgggt cgacactgaa gggctcgctt tctcccacga
     2581 gctttccgac atcgagtcgc tgaaccggcg ggcgatggcg ggagagctcg acgtcaccgc
     2641 ggtctccttc cacgcctacg cctacctcgc cgaccgatac ctcctccttc ctcacggcgc
     2701 gtcgttcggc gacggctacg gaccggtcgt cgtcgcccga caggcgatgt cgcgcgagga
     2761 gatcgcgtcg gggaaggtga tcgtcgcatc gcccggacgg tggacgacgg cggcgctcgc
     2821 tctcgcgctc tgggcgccgg gggtaccgac gaaggaggtc tccttcgacc gtatccagcc
     2881 ggacgtcgcg tcggggcgct tcgacgcggg agttctcatt cacgaagggc agctgacgta
     2941 ctccgaagaa ggcttgaccg gcgtcgtgga tctcgggtgg tggtggaaat cggagaccgg
     3001 cctaccgctc ccgctcggcg gaaacgtcgt gcggcgggat ctcggagaag accggatcgc
     3061 tcggatttcg cggtctctcc gccgctcgat cgagtacggc ctcgcccacc gcgaggacgc
     3121 cctttcccac gcactcgcct acgcccgggg cctcgaccgg acgcgcgccg atcggttcgt
     3181 cgggatgtac gtcaacgagt ggacccggga ctacggggtg gaaggacggc gagcggttca
     3241 gacgctcctc gaccggggcg ccgcggccgg gatcattccc cgggtccggg ccgaatatgc
     3301 ggctgactag acttcaggct ccgatcctct tccttcatct cgcggctgcc ggcctcgccc
     3361 tcggtccggc gctcttcttc ggcgcggtcg tcgccccggc cacgttccgg gtccttccca
     3421 cgcgcgacct ggcggcgagc ttgacgagcc ccgtcctctc cggcgcctgc ctcctggccg
     3481 aggtcgcgtt cggcgtcctc ttcatcaccg ggtggtggct gacgcgccgg gatgtcccgc
     3541 gggtgacgcg cagcctcctc acccgtttgc cggtggccgg gtttttcgcg gcgctcgcca
     3601 tccgcgagct gctcatcccg ccgatggaca agatccgcgc cgaggcgccg gggttgatcg
     3661 acaacctccc ggcggcggac ccgtcccgca tcctgctcga ccgataccat cgcctcgcga
     3721 ccggcttctt cggggccatg atcgccgtcg cggtcgcggt cctctttctg accgcgcggt
     3781 tcctctcgat cggagggaac gggcaacctc ccgccgcctc gcgtcccccg gtgccgaagc
     3841 tgctcgactt gtcgtaatcg gaatcagaga tccgaatcga tcaggcgctt cggcggcgga
     3901 tgagcgcctg tccgacgagc aacacccccg cggcaaccag agcccacggc cagaggttct
     3961 gaacctgccc gagcacgtcc aggatcggcg tgtgccgcgc gagcacgccg aagagcgcga
     4021 ctgcgatcag aatcactccg ggtaccagcg gagacggccg gcgcgcgccg cggcccgcga
     4081 cgcggtcgag gaaagccacc aggagaaacc cggcgccgag gccgaggacg atcgtcgccc
     4141 aggcgggaag caaccattcg agcggcgctc gaaggaggaa acccgccccg agcccgagga
     4201 ggatgccccc gggcagcacg ggccctcgaa atccccggat cgccgagagg gcgagaaaga
     4261 ccgtcccgag gagtacgagg agcgcgccgg gcccggagaa ccggaaggtt cgggagagga
     4321 ggaagtagcc ccccagaacg aggaggatga ccccgagggt caggctgcgc cggtctttca
     4381 cgcgcgcccc cgcggatgcg gtttgtcgcc ttcccaccga tagaagccct cgccggactt
     4441 ccggcccgtc cgcccttcgg cgaccatctt tcgcagcagt ggcggcggcg tgaacctctc
     4501 gccggaaagc tccgaggcga gcccttccgc gatcgcgagg cgaacgtcca ggccgacgag
     4561 gtcgctcgtc ttcagcggcc ccatgcggtg gccgtagccg agctccatcg cgcggtcgat
     4621 gtcttccggg agcgcgacgc cctcttcgac catccggatc gcctcgagcc cgagcgcgac
     4681 cccgaggcgc gaagttgcga agccggggga atctttcacc cggatcgcct cctttccgag
     4741 cgactggacc aggagaaggg cccgatccat cgtctcttcg gaagtgtcct cgccccgaac
     4801 gagttcgacg agaggcatcg cggcgaccgg gttgaagaaa tggatcccga tcacgcggtc
     4861 gcgacgtccc gtcgcgcggc cgatgcgcgt gatcgagagc gacgaggtgt tcgacgccag
     4921 gatcgcccgg tcctggcaga gatcgtcgag ctcgctcatc accatttgct tgacgtcgat
     4981 cgcttcgggc acggcctcga tcgcgaagtc gactcccgcg gcgtttcgga gccgctcgac
     5041 gatcttgagc agccgccgcc cgcgcaggac cgcctctgcc gcgacctttc cgcgctcggc
     5101 cgcgcgcgcc cacgaggact cgatggcgcc gcggacgtgc cgctgcgcct ccgggtcggc
     5161 atcgaagacg agcgtttcgt gtcccgagag ggcgcagagg cccgcgatcc cgcggcccat
     5221 cacgcccgct ccgacgacga ggatcttcat gcgcgaatcg taaatcggaa atcggaaatg
     5281 gggaatcgaa gggttcgatg accgactgca aactaccgat ttccgagcaa ttcctcggcg
     5341 ttgtcgaaga ggatcttccg cttcgcgtcg tcggagaggg ggagtgcaag gaagtcctcc
     5401 acgttcgcgc gcatcgagcg aattccgggg ctcgggaaat cggtccccca caggcaccgg
     5461 tccgagactt cctcgaggcg ggggatgtac tcgaggagct tgcgcggggg aatgcccgag
     5521 aggtcgaggt ggacgttgcg atgccgccgc gcgaggaaga cc
//
LOCUS       DASWAV010000216         6638 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U2665
            k141_172665, whole genome shotgun sequence.
ACCESSION   DASWAV010000216 DASWAV010000000
VERSION     DASWAV010000216.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960316
            Sequence Read Archive: SRR10260007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 6638)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6638)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:47:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,929
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6638
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_172665"
                     /isolate="SMAG_U2665"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.36444 N 132.31889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1113
                     /gene="gatB"
                     /locus_tag="VF958_04370"
     CDS             <1..1113
                     /gene="gatB"
                     /locus_tag="VF958_04370"
                     /inference="COORDINATES: protein motif:HMM:TIGR00133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HEX9148378.1"
                     /translation="LKTIRLVRIHLEEDAGKLLHENPFEDVPNTVSLVDWNRAGVPLV
                     EIVSEPDLRAPEEASAYLNELRRLLRYTEVSDADMEKGNLRCDANVSVRFSEGAPFGT
                     RVEIKNLNSIRFVARALEHEAARQADVLASGEPIVQETRLWDAAAGRTVLMRSKEEAH
                     DYRYFPEPDLGALIVDEAWIEETSRSLPELPRARRFRFVTQYGIAPGDAETLVSAREL
                     ADYFEDLAGRVAPRSAAVWVTGEVLRWMKERKLSPENARAFSVSPERLAGLLSLLESG
                     EISAASAKEVFGVMVDSPQDAAAIVRERGLGRISDTGALDAVVSEVVRENPSQVSLYR
                     SGKTQTFSWFVGQVMKRTGGRADPASVRDALTRALG"
     gene            1146..1850
                     /gene="ftsE"
                     /locus_tag="VF958_04375"
     CDS             1146..1850
                     /gene="ftsE"
                     /locus_tag="VF958_04375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020262047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HEX9148379.1"
                     /translation="MIQLFRVSKDFGAFRHALSDVSFTVERGEFIFLTGPSGAGKSTL
                     LKLLFREELPTSGQIVVNGRNIGVLPDSQVPYFRRTLGVVFQDFKLIARKTVFENLAF
                     VQSVLGLSRAEQKRRAYQALKRVGLHHRMNSRPPELSGGEQQRTAIALAIVNEPVLLL
                     ADEPTGNLDPVLSEEIMNLFSEINIRGTTVFVATHDVDLIRRMGKRVVTLEKGKLRER
                     DMARETLAREAPFLPG"
     gene            1847..2719
                     /locus_tag="VF958_04380"
     CDS             1847..2719
                     /locus_tag="VF958_04380"
                     /inference="COORDINATES: protein motif:HMM:NF037183.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease-like cell division protein FtsX"
                     /protein_id="HEX9148380.1"
                     /translation="MNSRYLFGEAWAIAKSGPRHTAVAVALIALAFYVPGLFALLSRN
                     LGRLAGSAREPTAVILTLDAASDSHGIAARVAQDPRVSRVTIVGSAAALERFRRTYPD
                     LGRAIAELDEAPFPPTIEVTLRYSAPARAAREIAAAARAWPGVDTAESEEEFAKKFRD
                     GVRLLRGAGLFLGGLLTLAAILSVASAVRLALDLHRDEIEIMRRMGATEAAIRAPFWL
                     NAAFEGLAGGAVALALLYATYRAAAHLLSREPHPVLALFWVGFLDLPTALALPAVGAA
                     AGFLGSLLSLGKKN"
     gene            complement(2729..3820)
                     /locus_tag="VF958_04385"
     CDS             complement(2729..3820)
                     /locus_tag="VF958_04385"
                     /inference="COORDINATES: protein motif:HMM:NF013740.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HEX9148381.1"
                     /translation="MSPPVVSPVLEEAHPQRTGGLVVLSTAAAVALLYYGRLLFVTLM
                     FALFLAFAMRPFVSLLERAGVPRVLAILLLIFLLGGVVVLLVINVTAQFNDLYDQLPR
                     YQIRIRELLSNVMNFVDRLRERMGSILPEDARGVREVKISESPLETTRAFMARIGATL
                     HFLLYAAAVPFLTFFMLKDREKFGRVLEGIFARSRRFGGDVTGAVSAKMTSYALGLSF
                     VMLIMASATTVALMILRVNYYYLLGPLAGLAILVPYVGVLFSTIPAIAVAFFQYGISK
                     AFIVLIVYTCLQFLEGNVLTPFIVGGKVRLFPLTVMVAFIFWGMLWGVAGAMLAVPMT
                     SAIKVVCEHVRGLEGFARLLGDPDPPPRS"
     gene            complement(3874..5226)
                     /locus_tag="VF958_04390"
     CDS             complement(3874..5226)
                     /locus_tag="VF958_04390"
                     /inference="COORDINATES: protein motif:HMM:NF012894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HEX9148382.1"
                     /translation="MKVKRSALLSLLFCLAGASAFAQIAPSNQGDIGLFTMPTADNPP
                     AGQLTLGLYGWLERLVAGELPTDADHRTRLYNHWAGVGSVGFGLTDHWSVFASPGWEM
                     RKSRGGWEGGIVNGLTFRAPFRIDEGRKVRIGTKVNFFSETGNGLRVALFGAAHVPVG
                     NASIRQDEQGIVADQINSRRADWEWGGVLTKGVFTAMGSYTLTGRHDQDVRTANRLRF
                     GAGVDVPIAPYLHVIGELDRTIFDGGDFPEEDYSLAYAGVRFWLGHTGLAISAALNAN
                     VDQIVQAQWGPAPIGGLVGITYVPWPPPPPPPVVVPPPPPAPIVEEAPAPVPPPPPAP
                     RTTSDEVYFDAGSARLTNIAKAILDGVALRMKNDLNSTAVISGYSDNLGSEEANLDIS
                     RRRAEAAREYLVTRHGIDPGRITTVGKGSAEAAYDNSTAEGRAKNRRALIVVTLVSGM
                     "
     gene            complement(5335..>6638)
                     /locus_tag="VF958_04395"
     CDS             complement(5335..>6638)
                     /locus_tag="VF958_04395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9148383.1"
                     /translation="DAGGAATQGLTRARPSLRHIGTALLLLAAGATTTVAQTPLPTPT
                     PTFPAPIESPGSPPAEPTPAAVSPAPTPAPEEPEKIGILPRLNVYLPEGRADIRFIKA
                     IRNSLFENQINYNFVSGDISAFLRYKYYGRSATSTLTFFDSIEFEELERFSNDFSRTR
                     GALYLQRRPINFYNRLFALVEFDRLTFSRPIDHPDANRTNVYVKLGYQLGTPSDERSN
                     AVVGEARDRVLSLFTAYREVGPHGRGFSAAATWGLDVLGGDYRYVKAEMEAFQAISLP
                     KKNRMIIRLHGGYFPYKKRVREEFDPLLGTPFSVPRYELFRLNGRQELKSYRGTERGP
                     NEAHLTVEYVVPVFTETPRRFLGLDWTSLYAVGYAGAGNVGNENRIYTRFRDYKVDAG
                     IGFETALSWRRYRAFLGALVAKTVVNGVGSGRLLVTFRTYR"
BASE COUNT         1123 a         2170 c         2283 g         1062 t
ORIGIN      
        1 ctgaagacga ttcgtcttgt tcgaattcac ctcgaggagg acgccggaaa gctcctgcac
       61 gagaacccct tcgaagacgt tccgaatacg gtgtcgctcg tcgactggaa ccgcgcagga
      121 gtgccgctcg tcgaaatcgt gagcgagccg gacctgcgcg cgccggagga ggcgtcagcg
      181 tacctgaacg agctgcgccg gctgctacgg tacacggagg tctcggacgc cgacatggag
      241 aaggggaacc tccgctgcga cgcgaacgtc tcggtccggt tctcggaggg ggctccgttc
      301 gggacgcgcg tcgagatcaa gaacctgaac tcgattcgct tcgtcgcccg cgcgctcgag
      361 cacgaggcgg cgcggcaggc ggacgtgctc gcctccggcg aaccgatcgt ccaggaaacg
      421 cggctctggg acgccgcggc gggacgcacc gtcctcatgc gaagcaaaga agaggcgcac
      481 gactacaggt attttccgga gcccgacctc ggcgctctca tcgtcgacga ggcctggatc
      541 gaggagacct cgcgctccct gcccgagttg ccgcgggcgc ggcgctttcg attcgtcacg
      601 caatacggga tcgcgcccgg cgacgccgaa acgctcgtgt ccgcgcgcga actcgccgac
      661 tacttcgagg acctcgcggg acgcgtcgct ccgcgctccg cggcggtgtg ggtcaccggc
      721 gaggttcttc gctggatgaa ggaacgaaag ctctctccgg agaatgcgcg cgcgttctcc
      781 gtctcgccgg agcgactcgc cggcctcctg tcgctactcg agtccggaga gatctccgcg
      841 gcgtcggcca aggaggtttt cggagtcatg gtggactccc cgcaggacgc cgccgcgatc
      901 gtccgcgagc gcggccttgg ccggatctcc gacaccggcg cgctcgacgc cgtggtctcc
      961 gaggtcgtgc gcgagaaccc ttctcaggtg agcctctacc gctccgggaa aacccagacg
     1021 ttcagctggt tcgtcggcca ggtgatgaag cggaccggcg gccgcgccga cccggcaagc
     1081 gtgcgcgacg cgctcacccg cgcgctcgga tgacgccgga ggagcgggcg gcagacgccg
     1141 ctccgatgat ccagctcttc cgcgtcagca aggacttcgg cgcgttccgt cacgcgctct
     1201 ccgacgtctc gttcacggtc gagcgaggcg agttcatctt cctgaccggg ccctcgggcg
     1261 cggggaagtc gacgctcctg aagctcctct tccgcgagga gcttccgacc tccggacaga
     1321 tcgtcgtgaa cggccggaac atcggcgtgc tgccggactc gcaagtcccg tacttccgcc
     1381 ggacgctcgg cgtcgtcttc caggacttca agctgatcgc ccgaaagacc gtgttcgaga
     1441 acctcgcatt cgtccagagc gtgctcggac tctcccgcgc ggaacagaag cggcgcgcgt
     1501 accaggcact gaagcgcgtg ggcctccatc accggatgaa ctcgcggccc cccgagctct
     1561 ccggaggcga gcagcagcgc accgcgatcg cgctcgccat cgtcaacgag ccggttctcc
     1621 ttctcgccga cgagccgacc ggcaacctgg acccggtgct ctccgaagag atcatgaacc
     1681 tcttctccga aatcaacatc cgggggacga cggtcttcgt cgcgacgcac gacgtcgacc
     1741 tgatccggcg catgggcaag cgcgtcgtga cgctcgagaa gggaaagctg cgggagcgcg
     1801 acatggcgcg cgagacgctc gcgcgggaag cgccctttct gccggggtga attcccgcta
     1861 cctcttcggc gaggcctggg cgatcgcgaa aagcggtccc cggcacacgg cggtcgctgt
     1921 cgcgctcatc gcgctcgcct tctacgttcc gggtctgttc gcgctgctct cgcgcaacct
     1981 cggccggctc gccggctccg cacgggagcc gaccgccgtc atcctcacgc tcgacgccgc
     2041 gtcggactcc cacgggatcg ccgctcgggt cgcgcaggac cctcgcgtct cccgcgtgac
     2101 gatcgtcggc agcgccgccg cgctcgaacg cttccgccgg acctaccccg acctcggcag
     2161 ggcgatcgcc gagctcgacg aggcgccgtt ccctcccacc atcgaggtca cgttgaggta
     2221 ctcggccccg gcgcgcgctg cccgcgagat cgcggcggcc gcgcgggcct ggccgggcgt
     2281 cgacacggcg gagtccgagg aggaattcgc gaagaagttc cgtgacggcg tgcggctcct
     2341 gcggggggcg ggtctcttcc tggggggcct cttgaccctc gccgccattc tgtcggtcgc
     2401 atccgcggtg cggctcgccc tcgacctcca ccgcgatgag atcgagatca tgcggcgcat
     2461 gggggcgacg gaagcggcca ttcgcgcgcc tttctggttg aacgccgcct tcgagggtct
     2521 cgcgggcgga gcggtcgctc tcgctctcct ctacgcgacg taccgcgccg cagcgcacct
     2581 gctgtcgcgc gagccgcatc ccgtgctcgc cctgttctgg gtcggtttcc tcgatcttcc
     2641 gacggcgctc gcgcttccgg cggtcggcgc cgccgccgga ttcctgggaa gtcttctctc
     2701 gctcgggaag aagaactgag cgcgacggct agcttcgggg cggcgggtcg gggtctccga
     2761 gcagccgcgc gaacccctcg agcccgcgga cgtgctcgca caccaccttg attgccgaag
     2821 tcatcgggac cgcgagcatc gcacccgcca cgccccacag catcccccag aagatgaaag
     2881 cgaccatcac cgtcagcggg aagagacgca cctttccgcc gacgatgaaa ggcgtcagca
     2941 cgttgccttc gaggaactgg aggcaggtgt agacgatgag aacgatgaac gccttgctga
     3001 tgccgtactg gaagaacgcg accgcgatcg cgggaatcgt cgagaagaga actccgacgt
     3061 agggcacgag gatcgcgagc ccggcgagcg ggccgagcag gtagtagtag ttcacgcgca
     3121 ggatcatcag cgcgacggtc gtcgccgacg ccatgatgag catgacgaac gagaggccga
     3181 gggcgtacga cgtcatcttt gccgaaaccg cgcccgtcac gtcgccgccg aagcgccggc
     3241 tcctcgcgaa tatcccctcg agaaccctgc cgaatttttc ccggtccttg agcatgaaga
     3301 acgtgagaaa cggaacggcg gcggcataga ggaggaagtg gagcgttgcc ccgatgcgcg
     3361 ccatgaaggc ccgtgtggtc tcgagcggcg actcggagat tttgacctcg cgcacgccgc
     3421 gcgcgtcctc cggcaggatc gagcccatcc gctcccgaag ccggtcgacg aagttcatta
     3481 cgttcgaaag aagctcgcgg attcggatct ggtagcgagg gagctggtcg tagagatcgt
     3541 tgaactgcgc cgtgacgttg atgacgagca ggacgacgac tccgccgagc aggaagatga
     3601 gaagaagaat cgccagtacc cgcggaacgc ccgccctctc gaggagcgag acgaagggcc
     3661 gcatcgcgaa tgcgaggaag agcgcgaaca tgagggtgac gaagagaaga cggccgtagt
     3721 agaggagggc gacggcggca gccgtcgaga ggacgaccag gccgcccgtc cgttgagggt
     3781 gcgcttcctc cagcaccgga gacacgacgg gcggtgacaa aaaagccccc tccggaagag
     3841 ggggctcgtt ggagctgtcc cgcgcctctc ggctcacatg ccggagacta acgtcaccac
     3901 gatcaaagcg cgacggtttt tcgcgcggcc ctcggcggtc gagttgtcat aggccgcttc
     3961 ggcggagccc ttgccgacgg tcgtgatcct ccccggatcg atgccgtgcc gggtcacgag
     4021 gtattcccga gcggcttccg cgcggcggcg ggagatgtcg agattcgcct cctcgctgcc
     4081 gaggttgtcg gagtacccgc tgatcaccgc cgtcgagttc aggtcgttct tcattcgcag
     4141 ggcgacgccg tccagaatcg ccttggcgat gttggtcaac cgcgcactcc ccgcgtcgaa
     4201 atagacctcg tcgctcgtcg tccgcggcgc cgggggcgga ggaggaaccg gcgcgggcgc
     4261 ctcttcgacg atcggcgccg gcgggggggg cggaacgacg accgggggcg ggggaggcgg
     4321 cggccagggg acataggtga ttcccacgag cccgccgatc ggcgccggac cccattgcgc
     4381 ctggacgatc tggtcgacgt tggcgttcag ggctgcggag atcgcgaggc ccgtgtgccc
     4441 gagccagaac cggacgcccg cataagctag cgagtagtcc tcttccggga agtcgccgcc
     4501 gtcgaagatg gtgcgatcga gctcgccgat tacgtgcagg tagggggcga tcggcacgtc
     4561 cacgcccgcg ccaaaccgga gccggttggc ggtccggacg tcctgatcgt gccggcccgt
     4621 cagagtgtag ctgcccatcg ccgtgaacac gcccttggtc aggacgccgc cccactccca
     4681 gtcggctcgc cgtgagttga tctggtcggc gacgatgccc tgttcgtcct ggcggatcga
     4741 ggcgttgccg acggggacgt gcgccgcccc gaacagagca acgcgcaatc cgttgccggt
     4801 ctcggagaag aagttcacct tcgtcccgat ccggaccttg cggccttcgt cgattcggaa
     4861 gggagcgcgg aaggtgaggc cgttgacgat tcccccttcc caacccccgc gggatttgcg
     4921 catctcccat ccggggctcg cgaagacgga ccagtggtcc gtcagtccga agccgaccga
     4981 accgacgccg gcccagtggt tgtagaggcg agttcggtga tcggcgtccg tcggcaactc
     5041 tccggccacg agccgctcga gccagccgta caggccgagt gtgagctggc ccgccggcgg
     5101 gttgtcggcg gtcggcatcg tgaagaggcc gatgtctccc tggttgctcg gagcgatctg
     5161 cgcgaaggcg gacgctccgg ccaggcaaaa aaggagagag aggagcgccg agcgctttac
     5221 cttcatgaaa gctcctttcg aacgggccgg agagaaatca gtgtgccgaa tagaataagc
     5281 caaaaacctt gtattgccaa acgttttgag cccggtggcc gccgtcccgg acctctaccg
     5341 gtacgttcga aaagtcacca gcaggcgccc gcttccgacg ccgttgacga ccgttttggc
     5401 caccagagcc ccgagaaagg cccggtaacg ccgccaggaa agggccgtct cgaacccgat
     5461 tccggcatcc accttgtagt cccggaatcg ggtgtagatc cggttctcgt ttccgacgtt
     5521 tcccgccccg gcatatccga cggcgtacag gctggtccag tcgaggccga ggaaccggcg
     5581 cggtgtctcg gtgaagaccg ggacgacgta ctcgacggtc aggtgggctt cgttcggtcc
     5641 ccgctcggtg ccgcggtagc tcttgagctc ctgccggccg ttcaggcgaa aaagctcgta
     5701 gcgggggacc gagaagggag tgccgaggag cgggtcgaac tcttcccgca cgcgcttctt
     5761 gtacggaaag tagccgccgt ggaggcggat gatcatgcgg tttttcttgg gtaaggagat
     5821 cgcctggaag gcctccatct cggccttgac gtaccggtag tcgccgccga ggacgtcgag
     5881 cccccacgtc gccgcggcgg agaatccgcg cccgtggggc ccgacctcac ggtacgccgt
     5941 gaagagagac aggacgcgat cccgcgcctc gccgaccacc gcgttggagc gctcgtccga
     6001 cggggtgccg agctgatagc cgagcttcac gtagacgttc gtccggttcg cgtcgggatg
     6061 atcgatcggt cgggagaacg tcaggcggtc gaactcgacg agcgcgaaga gccggttgta
     6121 gaagttgatc gggcggcgct ggaggtagag cgccccgcgc gtgcgggaga agtcgttcga
     6181 gaagcgctcg agctcctcga actcgatcga gtcgaagaac gtgagcgtcg aggtcgcgct
     6241 ccggccatag tacttgtagc gcaggaatgc cgagatgtcg ccggagacga agttgtagtt
     6301 gatctggttc tcgaagagcg aattgcggat cgccttgatg aaacggatgt ccgcgcggcc
     6361 ctcgggaagg tagacgttca ggcgcgggag gatcccgatc ttctccggct cctcgggagc
     6421 cggcgtcggc gcgggagaaa cggcggcggg agtcggctcg gccggaggcg agcccggcga
     6481 ttcgatggga gccgggaaag tcggggtcgg ggtcgggagc ggcgtctggg cgaccgttgt
     6541 ggtggcgccg gccgcgagca gcaggagggc cgtcccgatg tgcctcaagg agggacgcgc
     6601 tctcgtcagg ccttgggttg cagcaccacc ggcatctt
//
LOCUS       DASWAV010000217         4103 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U2665
            k141_172718, whole genome shotgun sequence.
ACCESSION   DASWAV010000217 DASWAV010000000
VERSION     DASWAV010000217.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960316
            Sequence Read Archive: SRR10260007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 4103)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4103)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:47:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,929
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4103
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_172718"
                     /isolate="SMAG_U2665"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.36444 N 132.31889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2598)
                     /locus_tag="VF958_04400"
     CDS             complement(<1..2598)
                     /locus_tag="VF958_04400"
                     /inference="COORDINATES: protein motif:HMM:TIGR03960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03960 family B12-binding radical SAM
                     protein"
                     /protein_id="HEX9148384.1"
                     /translation="MRFASLRPRLDPILPTVERPGRYVGLERNVVRKDLSAAEVTVAL
                     AFPDTYEIGMSHTGLKILYEIVNRRPDFACERTYAPWVDLEARMRGERIPLFTVESFA
                     PVADFDVVGFSLQSELNYSNVPNMLDLAGIPVWQRDRGEGDAIVLGGGPCTANPEPVA
                     DFFDAFLIGDAEEALPRFLDALKEARAAGLARRDVLASLAALDGIYVPSFYDVTYRED
                     GRITAIVRNDPRAPERARRTWVPVLKAEYYPDRPMVPSVEIVQDRLGLEVMRGCTQGC
                     RFCQAGYWYRPVRELDPADVVKMTKSFIAESGWSEVGLLSLSTADYSQIEPLVRCLAP
                     ELSERRVSISLPSLRAEAFSVGLADAVSEVRKSGFTFAPETGSDRLRRVINKTFTNAD
                     MIQAADVAFGRGWDLIKVYTMIGLPTETTSDLDELVTLVEGILARGRKHGRRKEVNVS
                     VGPFVPKSWTPFQWAPFDGVDVLERKLAYLKERFRRVRGARMKWHEPREAEIECTLSR
                     GDRRMARVLHAAWKSGVKFDGWSEHFRYDLWMRAFEAEGIAKESYLRAYDLDEVLPWD
                     VLDISINKRFLRVELVKAMKEMRTEDCKWGHCYACGVPGNGEDTVLASAMSGSLPTID
                     PAVTQSRDPAAYREVAKGAAYRQKGMPDLPSAVRRAPAGPPQVVRHRVTFEKTGDSRF
                     LSHRNTMDVLERAIRAAGLPARYSEGFNPHMKLSMGPALPLGLESRHEVFEVEGIAPF
                     GADAAREVNDKLPTGIRVLDIRELLPSEPALSRAVKSARYAVRLDSPDHLNRASNALA
                     NGWREMVPALRALSVERDELGARMTFEVNLDQSAGETSTAKKVLEALLAIAPEAQASL
                     SVIREATVLG"
     gene            complement(2622..3773)
                     /locus_tag="VF958_04405"
     CDS             complement(2622..3773)
                     /locus_tag="VF958_04405"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011142358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HEX9148385.1"
                     /translation="MFSFFAHLVCTKCGRVHERDIRFGVCPAPECGGPLFARYHDRPL
                     DRDAFADRPRTMWRWREMMPVEEPQNVVTLGEGGTPLLRADRLGTRAGFSDLWIKDES
                     GNPTGSFKARGLSVAVSKAKELGFQRIALPSAGNAGGAAAAYAARAGMSCDVFLPRDT
                     PPLFQAECERYGARVTLVDGLIDACGKLVAAGAAEGKWHDVSTLKEPYRVEGKKTMGY
                     ELAEDLRWELPDAVIYPTGGGTGLIGMWKAFEEMERLGWIGSKRPRMISVQAEGCAPI
                     VKAFAEGRTSAERWENAATYASGLRVPKAFADFLILADVRESSGDAVAVSDDEMRAAE
                     SEIGAAEGLFVCPEGAATWAAAKKLASAGRLDRRSRVVLFNTGTGFKYF"
BASE COUNT          676 a         1412 c         1375 g          640 t
ORIGIN      
        1 gccgaggacg gtcgcttcgc gaatcaccga gagcgacgcc tgtgcttcgg gtgcgatcgc
       61 gaggagcgct tcgaggacct tcttcgcagt cgacgtctct ccggcggact ggtcgaggtt
      121 gacctcgaac gtcatgcgcg cgccgagctc gtctctttcg accgacagcg ctctcaaggc
      181 cggcaccatc tcgcgccagc cgttggcgag cgcgttactc gcccgattca ggtggtcggg
      241 agagtcgagc cgcacggcgt aacgcgcgga cttgacggcc cgcgagagcg caggctccga
      301 gggaaggagt tcgcgaatgt cgaggacccg gatccccgtg ggcagtttgt cgttgacctc
      361 gcgcgcggcg tcggccccga agggcgcgat cccctcgact tcgaagacct cgtgccggct
      421 ctcgagaccg agcggcagcg cggggcccat cgaaagcttc atgtgcgggt tgaagccctc
      481 cgagtaacgc gccgggaggc cggccgcgcg gatcgcccgc tcgagcacgt ccatcgtgtt
      541 gcggtgagag aggaaacgcg agtcgcccgt cttctcgaac gtcacgcggt gccggacgac
      601 ctgcggcggc cccgccggag cgcgccggac ggcggaggga agatcgggca tccccttttg
      661 ccggtaggcg gcgcccttcg cgacctcacg gtaggccgcg ggatcccggg actgcgtgac
      721 cgccgggtcg atcgtcggga ggctgccgga catcgcgctc gcgagcaccg tgtcctcgcc
      781 gttgcccggc acgccgcacg cgtagcagtg cccccacttg cagtcctcgg tgcgcatctc
      841 cttcatcgcc ttgacgagct cgacgcggag gaacctcttg ttgatcgaga tgtcgagcac
      901 gtcccacggc aggacctcgt cgaggtcgta cgcgcgcagg taggactctt tcgcgattcc
      961 ttctgcctcg aaggcgcgca tccagaggtc gtagcggaag tgctcgctcc agccgtcgaa
     1021 cttcacgccg gacttccagg ccgcgtggag gacgcgcgcc atgcggcggt cgccgcgcga
     1081 gagcgtgcac tcgatctcgg cctcgcgcgg ctcgtgccac ttcatccggg cgccgcgcac
     1141 gcggcggaag cgctccttca ggtacgcgag ctttcgctcc aagacatcga cgccgtcgaa
     1201 cggcgcccac tggaacggtg tccaggactt cggcacgaac ggtccgacgg agacgttgac
     1261 ctccttgcgg cggccgtgct tgcgtccccg ggcgaggatg ccctcgacga gcgtgaccag
     1321 ctcgtccaag tccgacgtcg tctccgtcgg gaggccgatc atcgtgtaga ccttgatgag
     1381 gtcccagccg cggccgaaag cgacgtcggc cgcctggatc atgtcggcgt tcgtgaaggt
     1441 cttgttgatg acgcggcgca gccggtccga cccggtctcg ggggcgaacg tgaagccgga
     1501 cttgcggacc tcggacaccg cgtcggcgag cccgaccgag aacgcctcgg cgcgaagcga
     1561 cggcagcgag atcgagacac gccgctccga aagctcgggc gcgagacagc gcacgagcgg
     1621 ctcgatctgg gagtaatcgg cggtcgagag cgagagcagt ccgacctcgc tccagcccga
     1681 ctccgcgatg aacgacttcg tcatcttgac gacgtcggcg ggatcgagct cgcgcaccgg
     1741 gcggtaccag tagcccgcct ggcagaagcg gcagccctgg gtacatcccc gcatcacctc
     1801 gaggccgagc cggtcctgga cgatctcgac cgaggggacc atcgggcgat cggggtagta
     1861 ctcggccttg agcaccggca cccacgtgcg gcgcgcgcgc tccggggcgc ggggatcgtt
     1921 gcggacgatc gcggtgatcc gaccgtcttc ccggtaggtg acgtcgtaga acgaggggac
     1981 gtaaatgccg tcgagcgccg cgaggctcgc gagcacgtcc cggcgggcga ggccggccgc
     2041 ccgcgcctcc ttcagcgcat cgaggaaccg cggcagcgcc tcttccgcgt cgccgatcag
     2101 gaaggcgtcg aagaaatcgg cgaccggctc ggggttcgcg gtgcacggac ctccgccgag
     2161 gacgatggcg tcgccctcgc ctcgatctct ctgccacacc gggatcccgg cgaggtcgag
     2221 catgtttgga acgttcgagt agttgagctc cgactggagc gagaagccca cgacgtcgaa
     2281 gtcggcgacc ggcgcgaacg attcgacggt gaagagcggg attcgctcgc cgcgcatccg
     2341 cgcctcgagg tccacccatg gcgcgtacgt ccgttcgcac gcgaagtcgg gtcggcggtt
     2401 cacgatctcg tagaggatct tcagccccgt gtggctcatg ccgatctcgt atgtatcggg
     2461 aaaggcgagc gcgacggtga cctccgcggc cgagaggtcc ttgcggacga cgtttctctc
     2521 gagcccgacg tagcggcccg gcctctccac cgtcgggagg atgggatcga gacgcggccg
     2581 cagcgaggcg aatctcatgg aacccgcgat tctaaccggg gctagaagta cttgaatccg
     2641 gttccggtgt tgaagagcac gacccgtgac cgccgatcga gtctcccggc gctcgcgagt
     2701 ttctttgctg ccgcccatgt cgcggcgcct tcgggacaca cgaagagccc ttcggcggcg
     2761 ccgatctcgc tctccgccgc gcgcatctcg tcgtcgctca ccgcgacggc atcgccgctc
     2821 gactcacgca cgtcggcgag aatgagaaag tcggcgaaag ccttcggcac ccggagaccc
     2881 gaagcgtagg tcgccgcgtt ctcccagcgt tcggccgagg tccggccctc ggcgaacgcc
     2941 ttcacgatcg gcgcgcagcc ctccgcctgc acggagatca tccgcgggcg cttcgagccg
     3001 atccatccga ggcgctccat ctcttcgaac gccttccaca tcccgatcaa cccggtgccg
     3061 ccaccggtcg ggtagatcac cgcgtcgggc agctcccagc ggaggtcctc cgcgagctcg
     3121 tagcccatcg tcttcttccc ctcgacgcgg tacggctcct tcaaggtcga gacgtcgtgc
     3181 cacttcccct ccgcggcgcc cgccgccacg agcttcccgc acgcgtcgat caacccgtcc
     3241 accagggtga cccgcgcccc gtagcgttcg cactccgcct ggaagagcgg gggcgtgtcc
     3301 cgcgggagga agacgtcgca cgacatcccg gcgcgcgccg cgtaggccgc agcagcgccg
     3361 ccggcgttgc ccgcgctggg gagcgcgatc cgttggaacc ccagctcttt cgccttcgac
     3421 accgcgaccg agagcccccg cgccttgaaa gagccggtcg ggtttcccga ctcgtccttg
     3481 atccacagat cggagaatcc cgcgcgtgtc ccgagacggt ccgcgcggag gagcggcgtt
     3541 cccccctcgc cgagcgtcac gacgttctgc ggctcttcca ccggcatcat ctcgcgccat
     3601 cgccacatcg tgcgcggccg gtccgcgaac gcatcgcgat cgagcggacg atcgtgatac
     3661 cgcgcgaaga gcggtccgcc gcactccggc gcgggacaca ctccgaagcg gatgtcgcgc
     3721 tcgtggacgc ggccgcactt cgtgcagacg agatgcgcaa agaaggagaa catcgcagga
     3781 aacctatcag cgatcagcgg gaatcgagat cggaattgcg gatcgcgtgc gcaccgatac
     3841 cgcgagagga ggcaagacag aatagcacca atccgaggat tggaccatgc ttgacgctcc
     3901 cgcaaaacgg aagcgataat cgccgcggtc atggaattga acgatcacct gcgcgacatg
     3961 ctgaaagagc tcggtcaggc gatcaacgag tcgatctccg gctcgggtcg cgtgcacgac
     4021 tccatccagc ggattcgcga cgagggttac aacctctaca tggttctcga tgcgaaggtc
     4081 ggcgtgaacc ggcgcgaggg gcg
//
LOCUS       DASWAV010000218         2641 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U2665
            k141_212444, whole genome shotgun sequence.
ACCESSION   DASWAV010000218 DASWAV010000000
VERSION     DASWAV010000218.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960316
            Sequence Read Archive: SRR10260007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2641)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2641)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:47:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,929
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2641
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_212444"
                     /isolate="SMAG_U2665"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.36444 N 132.31889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..818)
                     /locus_tag="VF958_04410"
     CDS             complement(<1..818)
                     /locus_tag="VF958_04410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M28"
                     /protein_id="HEX9148386.1"
                     /translation="MRRSVPLSPTRAAVLLVLLPTLAAPPLAGQERIDYDMVTRIRQE
                     GFRNSKVMEIASGLTDGIGPRLTGSPNMKRANEWTRDKLAGWGLANAHLESWGPFGRG
                     WSYDSVSIRMTSPDVAQLLAIPKAWSPGTNGPVRGKVVLAKLATKEDLEKQKGKLAGQ
                     IVLTGDMRDVRPQEKAASERYDEKALEDVWHYQIPPARTPFGPEEFRRRREFRRVLNA
                     FLEQEKPLAVIDPGALDGGTFRVQQGGSYKKDEPIGVPALVMEIEHYGRIVRLL"
     gene            complement(855..1103)
                     /locus_tag="VF958_04415"
     CDS             complement(855..1103)
                     /locus_tag="VF958_04415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9148387.1"
                     /translation="MKRQKGRPTKRVLATVQELLSVGKPGVTFEIWDRWRRKRRKIGT
                     LTVSVGGLRWWPRDSPNYRRLTWEQFAERFEGAHRRNG"
     gene            complement(1135..2052)
                     /gene="egtD"
                     /locus_tag="VF958_04420"
     CDS             complement(1135..2052)
                     /gene="egtD"
                     /locus_tag="VF958_04420"
                     /EC_number="2.1.1.44"
                     /inference="COORDINATES: protein motif:HMM:NF021530.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030745 - dimethylhistidine
                     N-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0052699 - ergothioneine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-histidine N(alpha)-methyltransferase"
                     /protein_id="HEX9148388.1"
                     /translation="MARDLALTPKQIQSKYLYDALGSSLFESICRLPWYRITRAESRL
                     LTRSASEMVDPLEDPVTLLELGCGSGEKIATLAEALRGRRRRVAIHLIDISPTALELS
                     ERALGALEHVSVVGHRATYEEGLRHAARDRPRTGGLLVLFLGSNIGNFDSPASQEFLA
                     EIRAALRPGDGLLLGADLVKPEKELMLAYDDPLGVTAAFNKNLLVRMNRELGANFDLT
                     KFEHRAVWNPLDRRVEMHLVSMTAQLVRIPRANVSVDFAKGEFIWTESSYKYEAAGII
                     DLVTGAGFRSHSQWVDPDARFALTLFMAE"
     gene            complement(2120..>2641)
                     /locus_tag="VF958_04425"
     CDS             complement(2120..>2641)
                     /locus_tag="VF958_04425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020066670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HEX9148389.1"
                     /translation="GEWVWRGMFGDVLLPPSWPVFVSHAEASAYAKWCGARLPTEAEF
                     HRAAYGTPSGSERSYPWGEELPDASRGNFDFARWDPAPVGSFPAGASAWGAQDLLGNG
                     WEWTSTVFAPFPGFEPMPSYPVYSADFFDGKHWVIKGGSFATSKTLLRRSLRNWFRGN
                     YPYVYATFRTVAD"
BASE COUNT          426 a          902 c          874 g          439 t
ORIGIN      
        1 tcgagcagcc gcacgattcg cccgtaatgc tcgatctcca tgacgagtgc cggcacgccg
       61 atcggctcgt ccttcttgta cgagccgcct tgctgcacgc ggaaggtgcc cccgtcgaga
      121 gcgcccggat cgatgacggc gagcggcttc tcctgctcga ggaacgcgtt gagcacgcgc
      181 cggaactcgc gccggcgccg gaactcttcg ggaccaaagg gcgtccgcgc gggggggatc
      241 tggtaatgcc agacgtcttc cagcgccttc tcgtcgtagc gctcggacgc agccttttcc
      301 tgcggcctca cgtcgcgcat gtcgccggtc aggacgattt gcccggcgag cttccctttc
      361 tgcttctcga ggtcctcttt cgtcgcgagc ttcgcgagga cgactttccc tcggacgggc
      421 ccgttcgttc cgggactcca ggccttggga atcgccagca gctgggcgac gtcgggcgag
      481 gtcatccgga tcgagaccga gtcgtagctc cagccgcggc cgaacggtcc ccagctctcg
      541 aggtgcgcgt tcgcgagtcc ccacccggcg agcttgtccc gggtccactc gttcgccctc
      601 ttcatgttcg gcgaaccggt caggcgcggg ccgattccgt ccgtcaggcc gctcgcgatc
      661 tccatcacct tcgagttgcg gaacccctcc tgccggatgc gggtgaccat gtcgtagtcg
      721 atgcgctcct ggcccgcgag cggcggagca gcgagggtgg gaaggaggac gagaaggacg
      781 gcggcgcggg tgggcgagag tgggacggat ctcctcacgg acgcgcggac cgtatcaccc
      841 ggtcttccgg gcggtcaacc gttccggcgg tgggctcctt cgaaccgctc cgcgaactgc
      901 tcccacgtca gccgccggta gttcggcgaa tcgcgcggcc accatcgaag accgccgacg
      961 ctcacggtca gagttccgat cttccttctc ttccgtctcc atcggtccca gatctcgaat
     1021 gtgacacccg gcttgccgac ggaaagcagc tcctgaacgg tcgcgagaac acgcttggtc
     1081 ggccgccctt tctgcctttt cattgcgtca cctccgcgtc gaggtgtccg gacctcactc
     1141 cgccatgaac agagtcagcg cgaaccgcgc gtccggatcc acccactggg agtggcttcg
     1201 gaagccggcg cccgtcacga gatcgatgat cccggccgcc tcgtacttgt acgagctctc
     1261 cgtccagatg aactcgccct ttgcgaaatc cactgaaacg ttcgcgcggg ggattcgcac
     1321 gagctgcgcg gtcatggata ccaggtgcat ctcgacccgc cggtcgaggg ggttccaaac
     1381 cgcgcgatgt tcgaacttcg tcaggtcgaa gttcgcgccg agctcgcggt tcatccgcac
     1441 caggaggttc ttgttgaacg cggcggtcac accgaggggg tcgtcgtagg caagcatgag
     1501 ctctttctcc ggcttcacga ggtccgcccc gagcaggaga ccgtctcccg gacgcagcgc
     1561 cgcgcggatc tcggcgagga actcctgcga ggcgggcgaa tcgaagttgc cgatgttgga
     1621 gccgagaaag agcacgagaa ggcctcccgt ccgcggtcga tcgcgggccg cgtgccggag
     1681 tccttcttca taagtcgcac gatgccccac gaccgagacg tgctcgagcg ctccgagcgc
     1741 ccgctccgag agctcgagcg cggtcggtga gatgtcgatc aggtggatcg cgacgcggcg
     1801 gcgcctcccc cgcaacgctt ccgcgagcgt ggcgatcttc tcgccgctcc cgcagccgag
     1861 ctcgaggagc gtcaccggat cctcgagcgg gtccaccatc tcgctcgccg atctcgtgag
     1921 cagccgcgac tccgcccgcg tgatccggta ccaggggagc cggcagatag attcgaagag
     1981 cgacgagccg agggcgtcgt agagatattt cgactggatc tgcttcgggg tgagagcgag
     2041 gtcccgcgcc acgtcgcggg cgaagtcgcg gatcgcgtcc ggaggcggcg gcgcgacgag
     2101 ggtgctcagc gggaaggtct caatcggcga ccgtgcgaaa ggtggcgtac acgtaggggt
     2161 agttgccgcg gaaccagttg cgaaggctgc ggcgcaggag ggtcttcgac gtcgcgaacg
     2221 agccgccctt tatcacccag tgcttcccgt cgaagaaatc cgcggagtac acgggatagg
     2281 acggcatcgg ctcgaacccg ggaaacggcg cgaacaccgt cgacgtccac tcccagccgt
     2341 tgccgagaag atcctgcgcg ccccaggcgc tcgccccggc cgggaacgac ccgaccggtg
     2401 ccggatccca tcgcgcgaag tcgaagttgc cgcgcgaggc gtcgggcagc tcctcgcccc
     2461 acggatagga acgctccgac cccgacggcg tcccgtaggc agcgcggtgg aactccgctt
     2521 cggtcgggag ccgggcgccg caccatttcg cgtacgcgga cgcctccgcg tgactgacga
     2581 agaccggcca cgagggtggg agcaggacat cgccgaacat cccccgccag acccactcgc
     2641 c
//
LOCUS       DASWAV010000219         2797 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U2665
            k141_412733, whole genome shotgun sequence.
ACCESSION   DASWAV010000219 DASWAV010000000
VERSION     DASWAV010000219.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960316
            Sequence Read Archive: SRR10260007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2797)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2797)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:47:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,929
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2797
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_412733"
                     /isolate="SMAG_U2665"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.36444 N 132.31889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..180
                     /locus_tag="VF958_04430"
     CDS             <1..180
                     /locus_tag="VF958_04430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9148390.1"
                     /translation="APYMIFPGRLDARVTGAPPAVSWGGLAPGSYQLIVSGASGESAY
                     PFSIAEGQTTTVQIR"
     gene            complement(177..689)
                     /locus_tag="VF958_04435"
     CDS             complement(177..689)
                     /locus_tag="VF958_04435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9148391.1"
                     /translation="MALALMLGTLLAIAGLLLTSIQGFAVASGLHALTPLAKTLMTRH
                     VGYAIPTVLFSLFSQSMVIFFFIGTGKLVKEETARYPEDERRRILRALADLKRRTSPP
                     ATFALLSAIAVFVLGGAVHTRALPSWTHLAASVGALVLHVWALAADWKAFGDNARLMS
                     DPRRFVRKEA"
     gene            790..2601
                     /locus_tag="VF958_04440"
     CDS             790..2601
                     /locus_tag="VF958_04440"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid--CoA ligase"
                     /protein_id="HEX9148392.1"
                     /translation="MTAEIRTLCDIFLSAASSGKPDLLISKVGGTWTPISGKDFGYTV
                     RALSLGLNSLGIQPGDRVAILSENRPEWAMADYAALCAGAWTVPIYPTLPPHQVAPLM
                     NDSGAKAVFVSSLEQLGKILTIRPQCPSIEYVITIEASAPAEPGFLSFHAVVDKGRPM
                     LDMSPGAFEQRAARVAADDVATIIYTSGTTGEPKGAMLTHRNFVSNAVAACQVVPINK
                     TGVALSFLPLSHVFERMLDYSYLYKHSSIAYAESIDKLKENLLEVNPHCFGAVPRVYE
                     KVQARILGKVEASGGLKKKLFNWAVRIGRERVSYEERGLTIPTALARKAKIADKLVFA
                     KIRHALGSRFQFAVSGGAPLARELAEFFLGAGVTIYEGYGLTETSPVIAVNGPKAWRL
                     GTVGRPLPGVEVALAPDGEILSRGPHIMKGYFNKPQATAEAIDAEGWFHTGDIGQIDG
                     DGFLSITDRKKDLIVLAGGKNVAPQPIENELKGSPFIATPIVLGDRHKFVAALLVPNF
                     DRLKEHVASAKLKVDWEHLDADPEVRRIFQSEIDVYNREKPHHEQIHAFAILSADLTI
                     EDGSITPTLKVKRRILESRYRDLIDSMYRAAERSHVA"
     gene            2591..>2797
                     /locus_tag="VF958_04445"
     CDS             2591..>2797
                     /locus_tag="VF958_04445"
                     /inference="COORDINATES: protein motif:HMM:NF012336.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase"
                     /protein_id="HEX9148393.1"
                     /translation="MSRDVVIVDGLRTPYARAGTLLKDVPAQELARIVTTELLARTGL
                     DPAELDHVIFGNIAQPPDAVNLSRV"
BASE COUNT          496 a          930 c          916 g          455 t
ORIGIN      
        1 gcgccgtaca tgatctttcc gggaaggctg gacgctcgcg tcaccggtgc gccgcccgcc
       61 gtttcctggg gcggcttggc tcccgggtcc taccagctga tcgtctcggg cgcgtccgga
      121 gagagcgctt atccgttctc gatcgcggag ggtcagacca cgaccgtcca gatccgctaa
      181 gcctccttcc gcacgaatcg gcgcggatcg gacatcagcc gcgcattgtc gccgaaggcc
      241 ttccagtccg cggcgagcgc ccacacgtgg agcacgaggg cgccgaccga cgccgcgagg
      301 tgcgtccacg atgggagagc gcgcgtgtgc acggcgccgc cgaggacgaa caccgcgatc
      361 gccgagagga gcgcaaaggt cgccggtgga gaggttcgcc gcttcagatc ggcgagggcc
      421 cggaggatgc gccgccgctc gtcctccggg tatcgggccg tttcttcctt gacgagtttc
      481 cccgtgccga tgaagaagaa gatgaccatc gactgcgaga agagcgagaa gagcacggtg
      541 gggatcgcat agccgacgtg ccgggtcatg agggtcttcg cgagcggtgt cagcgcgtgg
      601 aggcccgacg cgaccgcgaa tccctggatc gacgtgagca ggagcccggc gatcgcgagg
      661 agagttccga gcatcagcgc gagggccatc ggtcagaaag catagccccg ggacgagtaa
      721 tccctttgca tccttgaccc tctccggggg gcgagacaca atcgcgcgaa ctctggagag
      781 gggagggtca tgaccgccga gatccgcacg ctctgcgaca tctttctctc cgcggcgtcg
      841 tcgggaaagc ccgacctcct gatctcgaaa gtcggcggaa cgtggacgcc gatctccggg
      901 aaagacttcg gttacacggt ccgcgccctt tcgctcggcc tgaactcgct tggcatccag
      961 ccgggcgatc gggtggcgat cctgtccgag aaccgtccgg agtgggcgat ggccgactac
     1021 gcggctctct gcgccggagc ctggacggtc ccgatctatc cgactctgcc gccccaccag
     1081 gtcgcgcccc tcatgaacga ttcgggggcg aaggccgtgt tcgtctcttc cctggagcag
     1141 ctcggcaaga tcctgacgat tcgtccccag tgtccgtcga tcgagtacgt catcacgatc
     1201 gaggcgagcg cgccggcgga gcccggcttc ctgtccttcc acgccgtcgt cgacaagggc
     1261 cggccgatgc tcgacatgag ccccggcgcg ttcgagcaac gcgccgcgcg cgtggccgcc
     1321 gacgacgtcg cgacgatcat ctatacgtcc ggcacgacgg gcgagccgaa gggcgcgatg
     1381 ctgacgcacc gaaacttcgt ctcgaacgcg gtcgccgcct gccaggtggt tccgatcaac
     1441 aagacggggg tcgccctgtc gttcctgccg ctctcccacg tcttcgagcg gatgctcgac
     1501 tactcgtacc tgtacaagca ctcctcgatc gcgtacgccg agtcgatcga caagttgaag
     1561 gagaacctcc tcgaggtgaa cccccactgc ttcggcgccg tgccgcgcgt ctacgagaaa
     1621 gtccaggcgc gcattctcgg aaaggtggag gcgtccggcg gactcaagaa gaagctcttc
     1681 aactgggccg tccgcatcgg ccgggagcgc gtttcgtacg aggagcgcgg gctgacgatc
     1741 cccacggcgc tcgcgcgcaa ggcgaagatc gcggacaagc tcgtcttcgc gaagatccgg
     1801 cacgcgctcg gaagccgatt ccagttcgcc gtgtccggcg gcgcgccgct cgcacgcgag
     1861 ctcgccgagt tttttctcgg agccggcgtc acgatctacg agggctacgg attgacggaa
     1921 acttccccgg tcattgcggt caacggaccg aaggcgtggc ggctggggac ggtgggtcgt
     1981 ccgctgcccg gcgtcgaggt cgcgctcgcc cccgacgggg agatcctgtc ccgcggtccc
     2041 cacatcatga agggttactt caacaagccg caggcgacgg ccgaggccat cgacgccgaa
     2101 ggctggttcc ataccggcga tatcggccag atcgacgggg acggcttcct ctcgatcacc
     2161 gaccgcaaaa aggatctgat cgtcctcgcg ggcggcaaga acgtcgcgcc gcagccgatc
     2221 gagaacgagc tcaagggctc gccgttcatc gcgactccga tcgtcctggg cgaccggcac
     2281 aagttcgtcg ccgctctcct cgtcccgaat ttcgaccgcc tgaaggaaca cgtcgcgtcg
     2341 gcgaagctga aagtggactg ggagcacctc gacgccgatc cggaggttcg acgaatcttc
     2401 cagagcgaga tcgacgtgta caaccgcgag aagcctcacc acgagcagat ccacgcgttt
     2461 gccatcctgt ctgcggacct gacgatcgag gacggctcga tcacgccaac gttgaaggtc
     2521 aagcggcgga ttctcgagag ccgctaccgg gacctgatcg actcgatgta ccgggcagcc
     2581 gagaggtccc atgtcgcgtg acgtcgtcat cgtcgacggc ctccggaccc cctacgcgag
     2641 ggcaggaacg cttctgaagg acgttcccgc acaggagctc gcacggatcg tcaccaccga
     2701 gcttctcgcg aggaccgggc tcgatccggc ggagctcgat cacgtgatct tcggaaacat
     2761 cgcgcagcct ccggacgccg tgaatctctc gcgcgtg
//
LOCUS       DASWAV010000220         6034 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U2665
            k141_452364, whole genome shotgun sequence.
ACCESSION   DASWAV010000220 DASWAV010000000
VERSION     DASWAV010000220.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960316
            Sequence Read Archive: SRR10260007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 6034)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6034)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:47:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,929
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6034
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_452364"
                     /isolate="SMAG_U2665"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.36444 N 132.31889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            188..721
                     /locus_tag="VF958_04450"
     CDS             188..721
                     /locus_tag="VF958_04450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9148394.1"
                     /translation="MKLRPVLGAIVPVFAVTFSMLALACTSAPAAVPIQATPAEISAL
                     AGHWEGEYASKATGRLGSIVLDLIAGEDHAHGDVLMIPRGSNQPYRPARGSGGQSPGP
                     GEATQLLKIRFIRAEGARLSGTLDPYWDPDCNCEVTTMFVGEVRGDTVEGTFTSERTA
                     GRVFGTWKANRKPAAIR"
     gene            732..1853
                     /locus_tag="VF958_04455"
     CDS             732..1853
                     /locus_tag="VF958_04455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007982425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VIT1/CCC1 family protein"
                     /protein_id="HEX9148395.1"
                     /translation="MEAGAVERYLENWKGEQDGAALYDAVAASEDNPKLAEIYRRLAA
                     TERRHAQLWADKLHAAGVDAPRFRPSARTRVLAWLARRWGARFVLPTVVGLEQADRNK
                     YDAQPEAVAAGLPGDEHSHARLLTQIQRSGGGLAGQDVARLEGRHRAAGGNALRAAVL
                     GANDGLVSNLALVMGVAGAELQERTIIITGFAGLLAGAFSMALGEWISVKSSVELHQH
                     QMRTEAAEIEASPEEEAEELALIYQARGLDERESQALASRILTDKTGALDVMAREELG
                     IDPNEMGGSPWVAAWTSFALFAVGAIVPVLPFLFATGSSAILGSAAFSGVALFGLGAG
                     ITLFTARPVLASGMRQMLFGLIAAGGTWAIGRLIGVSVS"
     gene            complement(1936..3090)
                     /locus_tag="VF958_04460"
     CDS             complement(1936..3090)
                     /locus_tag="VF958_04460"
                     /inference="COORDINATES: protein motif:HMM:NF014728.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HEX9148396.1"
                     /translation="MPESQPPDRPDPDKLLRLAQREEAKARLKVYLGMAAGVGKTVRM
                     LEDAHAMRRAGVDVVLGLIEPHGRAETIERIRDLDVIPRRKVRYREVELEELDLDAIL
                     ARRPDVVVVDELPHTNVPGSRNEKRWQDVEEILKAGISVMTALNVQHLEGVQDVVRSA
                     TGIEIRELVPDRIVRLAESVVLIDIPVPELQERLRQGKIYPPDQAKLALQNFFRAELL
                     GRLRELALLQTVELPGRAAATVAVEEKEARARVAVAIPFEPDVARALILRGSRLAGRM
                     NTAWFVLYVRQKKEHPHNISAQEHRRLSENVQLAMSLGATVVTRESEDVVGTILDFLR
                     SEHISLLLVGRPTVHRGLVRRVVPSTLDRLIDRASGVDFFVVDAETPTLL"
     gene            complement(3107..3691)
                     /gene="kdpC"
                     /locus_tag="VF958_04465"
     CDS             complement(3107..3691)
                     /gene="kdpC"
                     /locus_tag="VF958_04465"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020647852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpC"
                     /protein_id="HEX9148397.1"
                     /translation="MKLLFRSALYVLVTTALFGGAYPAAVTLVAQLVWPEKANGSYVS
                     RDGNAVGSALIGQNFHRPEYLHPRPSAAGKDGYDGLTSGGRNKGATDAGLAQSVADAV
                     KAARADRPSDSRPVPPDLVTASASGLDPHLSPDAAFWQAARIAKARGVSEEQVLSVLR
                     RHVEARTLGILGEPRVNVLLANLDLDRSLPRAAR"
     gene            complement(3688..5715)
                     /gene="kdpB"
                     /locus_tag="VF958_04470"
     CDS             complement(3688..5715)
                     /gene="kdpB"
                     /locus_tag="VF958_04470"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020715566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031004 - potassium ion-transporting
                     ATPase complex [Evidence IEA];
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpB"
                     /protein_id="HEX9148398.1"
                     /translation="MARPERRKLFEKAITRTAIQDSFVKLNPRTQLQNPVMFVVYVGS
                     ILVSVLLVRDAVRGGQLLFDLQIALWLWFTVLFANFAEAMAEGRGKAQAAALRRGRTE
                     TMARLVKDGREETTPATSLRKGDRVVVETGGVIPGDGTVVEGIASVDESAITGESAPV
                     IREAGGDRSAVTGGTRVLSDRIVVEVTSNPGETFLDRMIALVEGAARQKTPNEIALSI
                     LLAGLTIIFLVATATLRPFAIYAGGVASTPVLIALLVCLIPTTIGALLSAIGIAGMDR
                     VLQHNVLAMSGKAVEAAGDVNVLLLDKTGTITLGNRHATDFFPCAGCTVKALAEASQL
                     ASLADETPEGRSIVVLAKEKFGLRGRPLEGMRFVAFSATTRMSGVDWNGRQIRKGSGD
                     AVAEWVRSRGGEVDRAVEEVVIKVAAEGGTPLLVAEGSRMLGVIALRDVIKAGMRERF
                     DRLRAMGIRTIMITGDNPLTAAMIARESGVDDFLAHATPEQKLALIRREQEGGNLVAM
                     TGDGTNDAPALAQADVGVAMNTGTQAAREAGNMVDLDSNPTKLIEVVEIGKQLLMTRG
                     SLTTFSIANDVAKYFAILPAMFAATYPALNALNVMHLATPQSAILSAVIFNAVIIVAL
                     IPLALRGVKYRPLGAARILSRNLLIYGIGGIVIPFVGIKLIDLVVAALGLA"
     gene            complement(5726..>6034)
                     /locus_tag="VF958_04475"
     CDS             complement(5726..>6034)
                     /locus_tag="VF958_04475"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004273184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpA"
                     /protein_id="HEX9148399.1"
                     /translation="GAGNNGSAFAGLNANTLWYNTTLGLTMFLGRYFMLIPILGIAGS
                     LAAKKKIPASAGTFPVTGPLFTFLLVGVIVIVGALTFFPAFSLGPIVEHLLMKAGKVF
                     "
BASE COUNT          990 a         1971 c         2089 g          984 t
ORIGIN      
        1 cggagcgagg atccgatacg gtccgaaggt ggctcccgca gaaagcctca ccggagcctc
       61 actgcgagcg gtcttcaccg gattcgtgcg ggaaacgccg cgcgcaagga aaccgcgcgg
      121 ggattatatg tgtagggcgg ataacagctc gtgagacggt gcgtatctgt cggagaggag
      181 gctgtacatg aaactccgcc ctgttctcgg cgcgattgtt ccggtcttcg ccgtcacgtt
      241 ctcgatgctg gcgctcgcat gcacctcggc tcccgccgcc gtgccgatcc aggctacgcc
      301 ggcggagatc tccgcgctcg cgggtcactg ggaaggggag tacgccagca aggcgaccgg
      361 gaggctcggg tccatcgttc tcgacctgat tgcgggagag gaccacgctc acggcgacgt
      421 cctgatgatt ccgcgcggct cgaaccagcc ctaccgaccg gcccggggtt ccggtggaca
      481 aagtcccggc ccgggcgaag cgacccagct cctgaagatc cgattcatcc gggccgaagg
      541 cgccaggttg agcgggaccc tcgatcccta ctgggacccg gactgcaact gcgaagtgac
      601 gaccatgttc gtcggcgagg ttcgcggaga cacagtcgag gggactttca cgtcggagcg
      661 caccgccggc cgagttttcg ggacctggaa ggccaaccgg aagcccgcag cgatccgata
      721 acatccgctt ggtggaagcg ggcgccgtcg agaggtacct cgagaactgg aaaggggagc
      781 aggacggcgc ggctctttac gacgccgtcg ccgcctccga ggacaatccg aagctggcgg
      841 agatctatcg gcgcctcgcc gcgaccgagc ggcggcacgc ccagttgtgg gccgacaaac
      901 ttcacgcggc cggcgtcgac gctccccggt tccgaccctc ggcccgcacg cgtgtgctcg
      961 cgtggctcgc ccgacgctgg ggtgcccgat tcgtcctgcc caccgtcgtc gggctcgagc
     1021 aggccgaccg gaacaagtac gacgcgcagc ccgaagcggt ggccgccggt cttcccggtg
     1081 acgagcactc tcacgcgcgg ctgttgacgc agatccagcg ctccggcggc ggccttgcgg
     1141 gacaggacgt cgcgcgactg gagggccgtc accgagcggc gggggggaac gcgctccgtg
     1201 cggcggtgct cggggcgaac gacggtctcg tttcgaatct cgcgctcgtc atgggcgttg
     1261 ccggcgccga gctgcaggaa cgcacgatca tcatcaccgg cttcgcgggt cttctcgccg
     1321 gcgcgttttc gatggcgctc ggagagtgga tctcggtcaa gagctcggtc gagctccacc
     1381 agcaccagat gcgcaccgag gccgcagaga tcgaggccag ccccgaggaa gaggcggagg
     1441 agctcgcgct catctaccag gcgcgcgggc tcgacgaacg cgagagccag gccctcgcga
     1501 gccgcatatt gacggacaag acgggcgcgc tcgacgtcat ggcgcgcgag gagctcggaa
     1561 tcgatccgaa cgagatgggc ggctccccct gggttgccgc gtggacgtcc ttcgcgctct
     1621 tcgcggtcgg cgcgatcgtg cccgtgttgc cgttcctctt cgcgacggga tcgtccgcca
     1681 tcctcgggag cgcggcgttc tcgggagtcg cgctcttcgg gctgggcgcc ggaatcactc
     1741 tcttcacggc gcgccccgtg ctcgcgtcgg gaatgcgcca gatgctcttc gggctcatcg
     1801 cggcaggggg cacgtgggcg atcggacggt tgatcggagt cagcgtcagt tgatcctgcg
     1861 gctccggagg tcgcgcgctt tgtcgagttc gctctgaact aggagccggg gctcgccccg
     1921 tagcgcgaac gcgcgtcaca agagcgtggg cgtctcggcg tccacgacga agaagtccac
     1981 acccgacgcg cggtcgatca agcgatcgag ggtggacggc accacgcgcc ggacgaggcc
     2041 gcggtggacc gtcggccggc cgacgagcag cagagaaatg tgctcgctcc gcaagaagtc
     2101 gaggatcgtg cccacgacgt cttcggactc gcgggtcacc acggtcgcgc cgagagacat
     2161 cgcgagctgg acgttctccg agaggcgtcg gtgttcctgg gcgctgatgt tgtgcgggtg
     2221 ctctttcttc tgccggacgt agagcacgaa ccaggcggtg ttcatccgtc ccgcgaggcg
     2281 cgagccgcga aggatgagcg cacgggccac gtctggttcg aacggaatcg caacggcgac
     2341 ccgggccctc gcctccttct cttcgactgc gacagtggca gcggcgcgcc cgggtagctc
     2401 gacggtctgg aggagagcca gttcgcgcag gcggccgagc agctccgcgc ggaagaagtt
     2461 ctgaagggcg agcttcgcct ggtccggcgg gtagatcttt ccctgacgca gcctctcctg
     2521 cagctccgga accgggatgt cgatcagcac cacggactcc gcgaggcgaa cgatcctgtc
     2581 gggtacgagc tcgcggatct cgattcctgt ggcggaccgc acgacgtcct ggactccctc
     2641 gaggtgctgc acgttcagcg cggtcatgac ggagatgccg gctttcagga tctcctcgac
     2701 gtcctgccac cgcttctcgt ttcgagatcc ggggacgttc gtatggggca gctcgtcgac
     2761 caccacgacg tcaggccgcc gcgcgaggat cgcgtcgagg tcgagctcct cgagctcgac
     2821 ctcccggtag cgcactttgc ggcgggggat cacgtcgaga tcccggatgc gctcgatcgt
     2881 ttcggcgcgg ccgtgcggtt cgatcagtcc gagcacgacg tccaccccgg cgcggcgcat
     2941 cgcgtgtgcg tcctcgagca tacgcaccgt cttcccgacg ccggccgcca tgccgaggta
     3001 gaccttgagc cgcgccttcg cctcctctcg ctgcgcgagg cggaggagct tgtcagggtc
     3061 ggggcggtcg ggcggctgcg actcgggcat ccgcgatccg cctagtctag cgagcggctc
     3121 ggggtagcga tcggtcgagg tcgaggttgg ccagcaggac gttcacgcgc ggctctccga
     3181 ggatgccgag cgtccgggcc tcgacgtggc ggcgaaggac cgagagaacc tgctcttcgg
     3241 aaacccctcg cgccttcgcg attcgcgcgg cctgccagaa agcggcgtcg ggggagaggt
     3301 gcgggtcgag gcccgatgcc gaggcggtca cgaggtccgg tggaaccggg cgggaatcgc
     3361 tggggcggtc ggcccgcgcg gctttgacgg cgtctgcgac cgattgcgcc agcccggcgt
     3421 ccgttgcccc tttgttgcgc cctcccgaag tgagaccgtc gtatccgtcc ttgccggcag
     3481 cggagggcct cgggtgaagg tactccggcc gatggaaatt ctgtccgatc agcgccgacc
     3541 cgaccgcgtt cccgtcccgg gagacgtacg agccattcgc tttttcgggc cagacgagct
     3601 gcgcgacgag agtcaccgcg gcgggataag cgccgccgaa gagcgccgtg gtgacgagga
     3661 catagagagc agagcgaaaa agaagcttca tgccaggccc agcgccgcga cgacgaggtc
     3721 gatcagtttg attccgacga acgggatcac gatccctccg atgccgtaga tcaggagatt
     3781 cctcgaaaga attctcgccg ctccgagcgg gcggtatttg acgccccgga gcgcgagcgg
     3841 gatcaaggcc acgatgatca cagcgttgaa gatcaccgcc gaaaggatcg cggactgcgg
     3901 tgtcgccagg tgcatgacgt tcagagcgtt caacgccgga tacgtcgcgg caaacattgc
     3961 cggaaggatc gcgaagtact tcgcgacgtc gttggcgatc gagaacgtgg tcagcgaccc
     4021 gcgcgtcatc aggagctgct tgccgatctc gacgacctcg atcagcttcg tcgggttcga
     4081 gtcgaggtcc accatgtttc ccgcctcgcg cgcggcctga gtgccggtgt tcatggcgac
     4141 gccgacgtcg gcctgggcga gggctggcgc atcgttcgtc ccgtcgccgg tcatcgcgac
     4201 gaggtttccg ccctcctgtt cccgtcggat gagcgcgagc ttctgctccg gagtcgcgtg
     4261 cgcgaggaag tcgtcgacgc ccgattcgcg cgcgatcatg gccgccgtca atgggttgtc
     4321 gcccgtgatc atgatcgtgc ggatgcccat cgcgcgcagc cggtcgaagc gctcgcgcat
     4381 gccagctttg atgacgtccc gcagagctat gacgccgagc atcctcgatc cctccgccac
     4441 gagaagagga gttccccctt ccgcggcgac cttgatgacc acctcttcaa cggcgcggtc
     4501 gacctcgccg ccgcgggatc ggacccactc ggcgacggcg tcgccggagc ccttacggat
     4561 ctgccggccg ttccaatcaa ccccggacat tcgggtcgtt gcggagaagg cgacgaagcg
     4621 catcccctcg aggggtcggc ctctcagtcc gaacttctcc ttggccagaa cgacgatcga
     4681 gcggccttcc ggtgtctcgt cagcgagcga ggcgagctgg gacgcctctg cgagcgcctt
     4741 caccgtacaa cccgcgcagg gaaagaagtc cgtcgcgtgg cggttgccga gcgtgatggt
     4801 cccggtcttg tcgagcagga gcacgttgac gtcccccgcc gcctcgaccg ccttccccga
     4861 catcgcgagc acgttgtgct ggaggacgcg gtccatgccg gcgatgccga tggccgagag
     4921 gagggcgccg atcgtcgtcg gaatgaggca gacgaggagc gcgatcagaa cgggagtgga
     4981 tgccacccct cccgcataga tcgcgaacgg ccgcagggtc gccgtcgcga cgaggaagat
     5041 gatcgtcagc ccggcgagaa ggatggacaa cgcgatctcg ttcggcgtct tctggcgcgc
     5101 ggcgccctcg acgagcgcga tcatgcggtc gaggaacgtc tcgcccgggt tcgacgtgac
     5161 ctcgaccacg atgcggtcgg atagaacccg cgtcccgccc gtcacggcgg aacggtcccc
     5221 gccggcttct cgaatgaccg gcgccgattc cccggtgatc gccgactcgt ccacggaggc
     5281 gatcccttcg acgaccgttc cgtcgccggg gatcacgccg cccgtttcca ccacaacgcg
     5341 gtcgccctta cgaagactgg tcgcgggggt ggtctcctcg cggccgtctt tgacgaggcg
     5401 cgccatcgtc tcggtccgac cccggcgcag cgcggccgcc tgtgcctttc cgcgtccctc
     5461 ggccatcgct tcggcaaagt tggcgaagag gaccgtgaac cagagccaga gcgcgatctg
     5521 gaggtcgaag aggagctgcc cgccccgcac tgcgtcgcga acgagcagga cgctgacgag
     5581 aatggacccg acgtagacga cgaacatgac ggggttctgg agctgcgtcc gcgggttcag
     5641 cttgacgaag gagtcctgta tcgcggtgcg ggtgatcgct ttctcgaaga gcttgcggcg
     5701 ttcgggtcgt gccagggcag cctcctcaga acaccttccc ggccttcatc aggaggtgct
     5761 ccacgatggg gccgagagag aacgctggga aaaacgtcag tgcgccgacg atcacgatca
     5821 cgccgacgag cagaaaggta aagagcgggc cggtcaccgg aaaggtgccg gcggaggccg
     5881 ggatcttctt cttggctgcg agcgatccag cgatcccgag gatcgggatc aacatgaaat
     5941 agcggcccag gaacatcgtg aggccgagcg tcgtgttgta ccagagcgtg ttggcgttca
     6001 gacccgcgaa cgccgaaccg ttgttgccgg cccc
//
LOCUS       DASWAV010000221         1648 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U2665
            k141_651203, whole genome shotgun sequence.
ACCESSION   DASWAV010000221 DASWAV010000000
VERSION     DASWAV010000221.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960316
            Sequence Read Archive: SRR10260007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 1648)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1648)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:47:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,929
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1648
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_651203"
                     /isolate="SMAG_U2665"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.36444 N 132.31889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            246..1475
                     /locus_tag="VF958_04480"
     CDS             246..1475
                     /locus_tag="VF958_04480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9148400.1"
                     /translation="MRHSAAIALLSFALFSCVALGQTVILGNLPSSPNFGFDQTPVTA
                     VDFSNPASQAATLNSAAFTWSVSPCPGAVKIKFFRPTPTGSLAFLTERGPFDVTSLTQ
                     FIFLTPTVDVQAGDLVGIARLTACGSPVGQSPGNPQGFIGFNSDVTFAVTTSQGTLFP
                     NATLSVQATGMVNLPPPSADPTAIVPVVGSTPGAVGQAFFRTSVQLHNPRTTPISGHL
                     VYHPQGASGSASDPSMFYSLQPGETRSIPDLLPSMGLTGLGSLDVVAEDSLPAPLLAV
                     RVFNDAGTLGTTGFSEEFVLPGDALAAGERGILVAPPDAALYRYNVGVRTLGAGATLS
                     ITVRNAAGTVTRTLTRTYPANYFEQRDSAAFLDGPAVAANDTITVQVVSGRAIVYGAT
                     IDNRTNDPSMQLARRAP"
BASE COUNT          246 a          610 c          505 g          287 t
ORIGIN      
        1 gcggcggaga tcactggcgc cgatcagttg tcctgacgcc cgctgcgggc gcgcctttcg
       61 tcgttgcgca gttgacccac aactcggcca cggagacggg ctcgaccggt ctttcctgcc
      121 gcggatttct ccgctcccat cttcggagcc tgctcgagcg gccgacttgc cgtgagcatc
      181 gaaattgctc caccccttca tcgaccgccc ggacagggcc gggcgtcgca accgggagct
      241 ccgccatgcg ccattccgcc gcaatcgccc ttctgtcgtt tgctcttttc tcgtgcgtcg
      301 cgctcggcca gacggtgatc ctcgggaacc tcccgtcgag tcccaacttc ggattcgacc
      361 agacgccggt gacggcggtc gacttctcga atccggcgag ccaggcggcg acgctgaact
      421 ccgcagcatt cacctggtcg gtgagcccct gccccggcgc cgtgaagatc aagttcttcc
      481 ggccgacgcc caccggcagc ctcgcattcc tgacggaacg cggaccgttc gacgtcactt
      541 cgctcacgca gttcatcttc ctgacgccga ccgtcgacgt tcaggcggga gatctcgtcg
      601 gaattgcccg cctcacggcc tgcggaagtc ccgtcggaca gagtcccggc aacccccagg
      661 gattcatcgg gttcaactcc gacgtgacct tcgcggtcac gacgtcccag ggcacgctct
      721 tccccaacgc gacgctctcg gtccaggcga cggggatggt gaatctcccg ccgccatcgg
      781 ccgacccgac tgcaatcgtt ccggtcgtcg gctcgacgcc gggcgcggtc ggccaggcgt
      841 tcttccggac ctccgtgcaa ctgcacaatc cccgcacgac gccgatcagc ggccacctcg
      901 tctatcaccc gcagggggcg agtggcagcg cgtcggaccc gtcgatgttc tattcgctgc
      961 agcccggcga aacgagatcg atcccggacc tgctcccgtc gatgggcctc acgggtctcg
     1021 gcagcctcga cgtcgtcgcc gaagacagcc tgccggcgcc gctcctcgcc gtgcgggtct
     1081 tcaacgacgc gggtacactc gggacgaccg ggttcagcga ggagttcgtc ctgcccggcg
     1141 acgccctcgc ggcgggagag cgcgggatcc tcgtcgctcc gcccgacgcc gcgctctacc
     1201 gatacaacgt cggggttcga acgctcggcg ccggagcgac tctttcgatc acggtccgca
     1261 acgcagccgg aaccgtgact cgcacgctca cgcggacgta cccggcgaac tacttcgagc
     1321 agcgggattc cgcagcgttc ctggacggac cggctgtcgc ggcgaacgac acgatcaccg
     1381 tccaggtcgt ctcgggccgg gcaatcgtgt acggagcgac gatcgacaac cggacgaacg
     1441 acccgagcat gcagctggca agacgggcgc cctgaggtct cccgccggtg ttaaataggg
     1501 actccaccaa gaggaggtgt cgatgtctca ccggctccgt tcgctcgcgc gatcgctcgc
     1561 cttctccggc ttcgcggtcg cccttctcgt ttttccggcg ctgtccgggc tcgcgcagca
     1621 gcccgcgccc tcgtccgctc cgtcgaag
//
LOCUS       DASWAV010000222         2429 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U2665
            k141_330657, whole genome shotgun sequence.
ACCESSION   DASWAV010000222 DASWAV010000000
VERSION     DASWAV010000222.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960316
            Sequence Read Archive: SRR10260007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2429)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2429)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:47:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,929
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2429
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_330657"
                     /isolate="SMAG_U2665"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.36444 N 132.31889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..989)
                     /locus_tag="VF958_04485"
     CDS             complement(<1..989)
                     /locus_tag="VF958_04485"
                     /inference="COORDINATES: protein motif:HMM:NF016800.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HEX9148401.1"
                     /translation="MKVRKALFPLLVVFALLSSQISAAYVVFVLLAAGWVVSGARLES
                     FRRALAHPLTGLIGVFALLVASSVVFSTDSRASLRALPSLTLFLLVPITIDLVDDLRR
                     ARTILIAVAASGSAMALFGLAQLAREGTNLQNRIHANLSHYMTLSGLTLLAGCVLLAF
                     LFEGRGRVRWLGAAALIPFTAMIFTLTRGAYVGAVAAVIAYLVLRRPRGLLLVLPALV
                     VVFLLVPRDVRHRAASIADPTDPTNRDRIAMARAGVRMIADRPIFGLGPEMVKPFYTL
                     YRDPDAPRWRVPHLHDNVLQIAAANGVFAAGAYLAMLALFFVRTGALLRRQRR"
     gene            complement(993..1247)
                     /locus_tag="VF958_04490"
     CDS             complement(993..1247)
                     /locus_tag="VF958_04490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012040647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trm112 family protein"
                     /protein_id="HEX9148402.1"
                     /translation="MAVDPELLAILVCPKTKGPLELVDLTDDTRRALVEKYREKFRDE
                     EPVVTQGLYSKEANLVYPIISDIPVMLIDEALPGDARRHA"
     gene            1291..2406
                     /locus_tag="VF958_04495"
     CDS             1291..2406
                     /locus_tag="VF958_04495"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEX9148403.1"
                     /translation="MRILQLVAGQKWTGAAAVVFDQTAALVAAGVEAQFGFVGDSPLA
                     SRLLPLGWARPLLSPARGPSRYFRDVRTLSATLGRERFDVVHCHSSHDHYVAAAARRG
                     QRAGSPLLARTIHNIEQARPDPFSRALFRNTDAFAFANRAIAERFGGDGPVHSPVVDA
                     DRFRPGDKPLERLRNLGVADGSFVVGTVGKIAVGRGHREAIEVASTLSPDVVLLHVGK
                     GERRPELESRAAALGAAGRNFWAGYAEDELPELYRAMDVFLFPASGSQQGQRAILEAM
                     SSGRPVVALDVPGVSDLVTHEKEGFIAPRPADLASALERLRRDPGLRAQMGALGRARA
                     REFGGESFAGSAKEFYARLLARKSMTSRGWSAGDTAG"
BASE COUNT          416 a          808 c          854 g          351 t
ORIGIN      
        1 ttcctgcgtt gcctgcgcaa gagagcaccc gtccgcacga agaacagagc gagcatcgcg
       61 agataggccc cggcggcgaa cacgccattc gcggcggcga tctgcaggac gttgtcgtga
      121 aggtgcggca cccgccagcg cggcgcgtcc gggtcccggt agagtgtgta gaaaggtttc
      181 accatttccg ggccgaggcc gaagatcggg cggtccgcga tcatgcgaac cccggcgcgc
      241 gccatcgcga ttcgatcccg attcgtcggg tcggtcgggt cggcgatcga ggctgcgcgg
      301 tgcctcacgt cgcggggaac gagaaggaac accacgacga gcgccggaag gacgagaagg
      361 agcccccgcg gcctccgcag cacgaggtag gcgatgaccg cagcgacggc gccgacgtag
      421 gcgccccggg tcagcgtaaa gatcattgcg gtgaagggga tcagtgcagc ggcacccagc
      481 cagcgcacgc ggccacgacc ctcgaagaga aaggcgagaa ggacgcaccc ggcgagcagc
      541 gtgagccccg agagggtcat gtagtgagag agattcgcgt gaatccggtt ctggagattc
      601 gtcccttcgc gcgcgagctg cgcgagcccg aagagcgcca tcgcactccc gctcgccgcg
      661 acagcaataa gaatcgtccg ggcgcggcgc aggtcgtcca ccaggtcgat cgtgatcggc
      721 acgaggagaa agagcgtgag gctcgggagg gctcgcaggc tcgcgcgcga gtccgtggag
      781 aacacgaccg acgacgcgac gagcagtgca aagactccga tgagccccgt cagcgggtgg
      841 gcgagcgccc gtcggaagct ctcgagccgc gccccggaaa cgacccaccc ggcggcgagg
      901 aggacgaaga cgacgtaggc ggcggagatc tgcgacgaga gaagggcgaa gacgacgagg
      961 agaggaaaga gagccttgcg gaccttcaat tctcaggcgt gccggcgggc atctcccggc
     1021 agcgcctcat cgatcagcat caccggaatg tcggagatga tcgggtagac gaggttcgcc
     1081 tccttcgaat agaggccctg cgtgacgacc ggctcctcgt cccggaactt ctcgcgatac
     1141 ttctccacga gcgcccggcg cgtgtcgtcg gtcaggtcga cgagctcgag agggcccttc
     1201 gtcttcgggc acaccagaat ggcgaggagc tcgggatcaa cggccatcgg cggcaagtct
     1261 aatcgagcgg agagctgata gcgtcttttc gtgcgcatcc tccaactcgt cgccggccaa
     1321 aagtggaccg gggccgcggc ggtggttttc gaccagaccg ccgccctggt cgccgcagga
     1381 gtcgaggcgc agttcgggtt cgtcggcgac agccccctgg cttcgagact tctgccgctc
     1441 ggctgggccc gacccctgct ctcccccgcc cgcggcccgt cgcgctactt ccgcgacgtc
     1501 cggacgctct ccgccacgct cggccgggaa aggttcgacg tggtgcactg ccactcctcg
     1561 cacgatcact acgtggctgc ggccgcgcga cgcggccagc gagccggaag cccgctgctc
     1621 gcgcgcacga ttcacaacat cgaacaagcg cgcccggacc cgttctcccg ggccctcttc
     1681 cggaacaccg acgcgttcgc tttcgcgaac cgcgcgatcg cggagcgctt cggaggcgac
     1741 ggccccgtcc actctccggt cgtcgacgcc gaccggtttc gacccgggga caagccgctc
     1801 gagcgtctgc gaaacctcgg cgttgccgac gggagcttcg tcgtgggcac ggtcggaaag
     1861 atcgcggtgg gccgggggca tcgcgaggcg atcgaggtgg cgtcgactct ctcgccggac
     1921 gtcgtgctcc ttcacgtcgg aaaaggggag cggcgtcccg agcttgaatc gcgtgcggcg
     1981 gcgctcggcg cggccggccg caacttctgg gccggctatg cggaggacga attgcccgag
     2041 ctctatcgcg cgatggacgt cttcctcttc cccgcctcgg gatcgcagca gggacagcgc
     2101 gcgatactcg aggcgatgtc ctccggccgg cccgtcgtcg cgctcgatgt cccgggagtt
     2161 tccgacctgg tgacgcacga aaaggagggc ttcatcgccc cgcgccccgc cgacctcgcg
     2221 tccgcgctcg agcgactgcg acgcgacccc gggctgcggg cgcagatggg agcgttgggc
     2281 cgcgcacgcg cgcgagaatt cggaggagag tcgttcgccg ggtctgcgaa ggagttctac
     2341 gcgcgcctgc tcgcgagaaa gtcgatgacc tctcgggggt ggtccgccgg tgacaccgcg
     2401 ggataggccg cggtgatcct tcccttctc
//
LOCUS       DASWAV010000223         2578 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U2665
            k141_413049, whole genome shotgun sequence.
ACCESSION   DASWAV010000223 DASWAV010000000
VERSION     DASWAV010000223.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960316
            Sequence Read Archive: SRR10260007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2578)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2578)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:47:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,929
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2578
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_413049"
                     /isolate="SMAG_U2665"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.36444 N 132.31889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1055
                     /locus_tag="VF958_04500"
     CDS             <1..1055
                     /locus_tag="VF958_04500"
                     /inference="COORDINATES: protein motif:HMM:NF024491.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phospholipase D-like domain-containing protein"
                     /protein_id="HEX9148404.1"
                     /translation="GAGAFAAMDQAIDGARTEILAESYIWKDDATGRRTLDALGRAAA
                     RGVAVRVLADAWGSFSTRRRYWREMRERGIEVRLFNPLFPHLFAQPLRDHRKILVVDR
                     TIAFTGGMNIADEYCVGTAGESGRAWRDTHARVEGPAAWEMTTVFSEAWSAAGGSDLE
                     LQPLAPPEEGGARVLVLDSRPGRGHGETASVLAAIVAAARERIWITNAYFAPQRRAIE
                     ALAGATRRGVDVRLLLPGLTDVPIVRHAGHGLYDALRAAGVRIYEYQPAILHAKTMVA
                     DDFVSFVGSSNLDFRSFHFNSECNFLVLDGDVGRTMSAAFERDLLSSREIDESWTRTF
                     RHRLGDSLARRLGPLL"
     gene            complement(1052..>2578)
                     /locus_tag="VF958_04505"
     CDS             complement(1052..>2578)
                     /locus_tag="VF958_04505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012876058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-linked oxidase C-terminal domain-containing
                     protein"
                     /protein_id="HEX9148405.1"
                     /translation="YDAADLVIEHGGSISGEHGDGQSRAELLPRMFGPELVEGFREFK
                     RIWDPDGLMNPGKIVDPYRADQNLRHTSPAGRSHVLINPAFAYAKDAGDFSRALQRCV
                     GVGECRRAKGGVMCPSFMVTREEKHTTRGRARLLDEMTRGEVLTGGWKEEAVREALDL
                     CLACKGCKSDCPVGVDVAAWKAEFLSRYYRGRLRPAAAYSMGLVHRWARVASSAPAVA
                     NFFTQTPGVSGLAKALGGIAPERRLPRFATETFRGWFNRRRQRAARAEPRGGRAVLLF
                     PDTFTNHFSPGIGRAAVAVLEGAGFAVDIPGADACCGRPLFDRGMLDTARRLLAKTLD
                     VLSPAIALGVPILVLEPSCAAVFKDELPELFPGDARAARLAENTLLLAGLLESAPGPD
                     VPSVPRRALVQFHCHQRAVFGVETDRRLLSSMTTAVDVPDSGCCGMAGAFGFEKSHYA
                     MSVAIGERVLFPEVRKAAPETLVVADGFSCREQIAQGTGRRALHLAEVMRLAMEEETP
                     "
BASE COUNT          392 a          896 c          917 g          373 t
ORIGIN      
        1 aaggggccgg ggcgttcgca gccatggacc aggcgatcga cggcgcgcgg accgagatac
       61 tcgccgagtc ctacatctgg aaggacgacg caaccgggcg ccggacgctc gacgcgctcg
      121 gacgcgctgc ggcaaggggc gtcgccgttc gcgttctcgc cgacgcctgg ggctccttct
      181 ccacgaggcg acgctactgg agagagatgc gcgagcgggg gatcgaggtc cgcctgttca
      241 acccgctctt cccccacctc ttcgcgcagc cgctgcgcga ccaccgaaag atcctcgtcg
      301 tcgaccggac gatcgccttc acgggcggga tgaacatcgc cgacgagtac tgcgtgggaa
      361 cggccgggga gtccggccgg gcctggcggg acacgcacgc gcgggtcgag ggcccggccg
      421 cctgggaaat gacgacggtc ttctcggaag cctggagcgc ggccggcgga agcgacctgg
      481 agctccagcc gctcgctcct ccggaggaag ggggcgcgcg cgtgctcgtg ctcgactccc
      541 gccccggccg cgggcacggc gagaccgctt cggtcctcgc ggcgatcgtc gccgcggcgc
      601 gcgagaggat ctggatcacg aacgcgtact tcgcgcccca acgtcgagcg atcgaggcac
      661 tggcgggggc gacccggagg ggcgtggacg tccggttgct tctgcccgga ttgaccgacg
      721 tccccatcgt tcgccacgcc gggcacggcc tgtacgacgc tcttcgcgcc gccggtgtcc
      781 ggatctacga atatcagccg gcgatccttc acgccaagac gatggtggcg gacgacttcg
      841 tctccttcgt cgggtcgagc aatctcgact ttcggtcctt ccacttcaac tcggagtgca
      901 acttcctcgt gctcgacgga gacgtcggcc gcacgatgtc cgcggcgttc gaacgcgacc
      961 tcctctcgtc gcgggagatc gacgagagct ggacccggac gtttcggcac cggctgggag
     1021 actcgctggc gcgccggctc ggaccgcttc tctagggggt ctcttcctcc atcgcgagac
     1081 gcatcacctc ggcgaggtga agggcgcggc gcccggtccc ctgcgcgatc tgctcgcggc
     1141 agctgaagcc gtcggcgacg acgagggtct ccggcgcggc tttgcggacc tccgggaaga
     1201 ggacccgctc gccgatcgcg accgacatgg cgtagtgcga cttctcgaaa ccgaacgcgc
     1261 cggccatccc gcagcacccc gagtcgggca cgtcgacggc ggtggtcatg gacgagagga
     1321 gccgtcggtc cgtctcgact ccgaagaccg ctcgctgatg gcagtggaac tgcacgagcg
     1381 cgcgtcgggg tacggaaggg acgtccgggc ccggagcgga ctcgaggagc ccggcgagca
     1441 ggagcgtgtt ctcggcgagc cgggcggccc gcgcgtcccc gggaaagagc tccggcagct
     1501 cgtctttgaa aaccgccgcg cagctcggct cgagcacgag aatcgggacg ccgagggcga
     1561 ttgccggcga gaggacgtcg agcgtcttcg ccagcaggcg gcgcgccgtg tcgagcatcc
     1621 cccggtcgaa gagcggcctt ccgcagcacg cgtcggcgcc cgggatgtcg accgcgaatc
     1681 cggcgccttc caggacggcg accgccgccc ggccgatccc gggcgagaag tggttcgtga
     1741 acgtgtcggg aaagaggaga acggctcggc cgccacgcgg ctccgccctt gccgcgcgct
     1801 gccgccgccg gttgaaccag ccgcggaacg tctccgtcgc gaaacggggc agtcggcgct
     1861 cgggcgcgat tccgccgagc gccttcgcga gcccggagac tccgggagtc tgcgtgaaga
     1921 agttcgcgac cgcgggcgcc gacgacgcga cccgggccca ccggtgcacg agacccatcg
     1981 agtacgccgc cgcggggcgg agccgccctc ggtaatagcg cgagagaaac tccgccttcc
     2041 atgccgcgac atcgacgccg acggggcagt ccgacttgca ccccttgcac gcgaggcaga
     2101 ggtcgagcgc ctcgcggacc gcctcctcct tccacccgcc ggtcaggact tcgccgcggg
     2161 tcatctcgtc taagagccgt gcccgaccgc gcgtcgtgtg cttctcctcg cgggtgacca
     2221 tgaagctcgg gcacatgacg ccgccctttg cacggcggca ctcgcccacg ccgacgcagc
     2281 gctggagcgc gcgcgagaag tcgccggcat ctttcgcata agcgaatgcg gggttgatta
     2341 agacatgcga ccgtccggct ggggacgtgt ggcgcaggtt ctggtcggcg cggtaggggt
     2401 cgacgatctt gccggggttc atgagtccgt cggggtccca gatcctcttg aactcgcgga
     2461 agccttcgac gagctcgggc ccgaacatcc ggggaaggag ctccgcgcgc gactgtccgt
     2521 cgccgtgctc gccggaaatc gagccgccat gctcgatcac gaggtcggcg gcgtcgta
//
LOCUS       DASWAV010000224        11548 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U2665
            k141_12722, whole genome shotgun sequence.
ACCESSION   DASWAV010000224 DASWAV010000000
VERSION     DASWAV010000224.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960316
            Sequence Read Archive: SRR10260007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 11548)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11548)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:47:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,929
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11548
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_12722"
                     /isolate="SMAG_U2665"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.36444 N 132.31889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1892)
                     /locus_tag="VF958_04510"
     CDS             complement(<1..1892)
                     /locus_tag="VF958_04510"
                     /inference="COORDINATES: protein motif:HMM:NF012722.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing protein"
                     /protein_id="HEX9148406.1"
                     /translation="MSDEKPPQTPRGEKRVPTLVAELQRHRKENRLVVFLALAVLVVW
                     SASSVLRQRAEEMEPATITRGLLLFVLSYLNVTLIAAVLFVLCRTLIKVWLEQRRGVL
                     GSRFKTRLLVTYIGLTAIPIGLLFFTATGFLQRSIDRWFSSPVREVVGRARSVEDMAE
                     RRITDAAVEDAKSLSRLPVEGVSADRLAESLEEFRRQERLDSVEVYRGSAREGTAADF
                     GDLAPLPAETLETAFSRGEALKVEVLADGSHLFRAARRSGTRVFAAGVRVPPSDARAM
                     RAIASAWSDYQKLEVQKPAIKAANVSTFLLITLAVLLASIWTGMTLARRITGPIAALA
                     ESTRRIRAGDFTARVDVAAADELDVLVDSFNSMAAGLQEARDATLRSNEELQEINRRL
                     DLERGLRSTVLGSVTTGVLAFAASGRATVANPAARSLLRLGDAEVTLEGLRGRPELAP
                     LVRLLEKAEEGAAPETPRELILSGDGGERRLEIALRPMSSASPGNRGGWVVAVEDTTH
                     VAREQKLAAWSEVARRVAHEIKNPLTPIRLSAERILRRLRAGEPDLPEAIERGTRVIV
                     DEVGVLKSLVDEFSRFARLPEMKREAVDLAGLAGSAVRLFEGVRDGVQVRVESSLSRD
                     RVQLDP"
     gene            complement(1889..2455)
                     /locus_tag="VF958_04515"
     CDS             complement(1889..2455)
                     /locus_tag="VF958_04515"
                     /inference="COORDINATES: protein motif:HMM:NF025692.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4390 domain-containing protein"
                     /protein_id="HEX9148407.1"
                     /translation="MLRRTIAAVLVGSVFAGGPASGQTDSGPRVRELRVESRGRDVFV
                     TFALAGALNPELARKIEAGLETAIRYDIRLYRRYPYWFDDLVERRKYRVAVTFDPVTR
                     EYGVDETLDGKPLGRNTTRDFPEAAKLLLSRENLLAFHIQRGRPTRNLYVQMRASFDA
                     GYVFTIIPVDSRTPWKKSKRFNIRAVTP"
     gene            complement(2555..3445)
                     /gene="lpxC"
                     /locus_tag="VF958_04520"
     CDS             complement(2555..3445)
                     /gene="lpxC"
                     /locus_tag="VF958_04520"
                     /EC_number="3.5.1.108"
                     /inference="COORDINATES: protein motif:HMM:TIGR00325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008759 - UDP-3-O-[3-hydroxymyristoyl]
                     N-acetylglucosamine deacetylase activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-acyl-N-acetylglucosamine deacetylase"
                     /protein_id="HEX9148408.1"
                     /translation="MARAQTTLRTSVSIEGVGLHSGHPVRASFRPAPAGHGIVFRRLD
                     RPDTLVPARVESASTFDYATTLSVDGVSVGTVEHVLSAAYGEGLDNCVIELDGPEVPI
                     LDGSALPFVRLFHAAGFERLDSPVESLASSGPVEVSRGGRGVSFQPDGPGLTITYEID
                     FPHPVVGRQELTVAIRPEEYSTRIAPARTFGFAKDVDALRSRGLARGGSLHNAVVLDE
                     EGVLSGPLRFRDEFVRHKILDLVGDLALLGVSLHGHIHARRAGHALHIEFARALAAAS
                     HGRRQAKDVAVERPRRRVSR"
     gene            3705..4187
                     /gene="smpB"
                     /locus_tag="VF958_04525"
     CDS             3705..4187
                     /gene="smpB"
                     /locus_tag="VF958_04525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006680933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HEX9148409.1"
                     /translation="MKTKQRTADDGVNTVATNRKAFHDYFIEDRTEAGLVLTGTEVKS
                     LREGRAQLKDSYVQFRAGEAYLIGAHISPYSAGSWSNHLPERERKLLLHRREIERWAA
                     AVATRGVACVPLSLYFKGPRAKAQIALVRGKKQHDKRDAIRERDLKREADRAMRERSR
                     "
     gene            complement(4189..4605)
                     /locus_tag="VF958_04530"
     CDS             complement(4189..4605)
                     /locus_tag="VF958_04530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008894007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MOSC domain-containing protein"
                     /protein_id="HEX9148410.1"
                     /translation="MSSVDSAHAVPGRGLEGDRYFSKSGTYSHRDGPDREITLIEIEA
                     VEALERDYGIRLEPNETRRNVATRGVALNHLVGARFRVGETVLLGLRLCEPCGYLERK
                     CGKPVREGLVHRGGLRAQILTKGWLRVGDKVDVQPA"
     gene            4685..5197
                     /locus_tag="VF958_04535"
     CDS             4685..5197
                     /locus_tag="VF958_04535"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HEX9148411.1"
                     /translation="MIRRARPGDLRRLWELDRICFEPGIAYSRAELRRFLDLPRAKCL
                     VAESAGAIDGFAVGYPDPPDLARVVTLDIHPSARRRGLGKELLERLLAELGEAGGGRA
                     VLEVDVRNTGAIAFYRRLGFSTMRRIPDYYGPGLDAFEMVRGPGVTRSPEAPGGGAEG
                     SEIRRPPRGD"
     gene            complement(5124..8696)
                     /locus_tag="VF958_04540"
     CDS             complement(5124..8696)
                     /locus_tag="VF958_04540"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HEX9148412.1"
                     /translation="MSLRVLLVAASPGDAEPLVDELTRAGYEVAWEVVRDGSEMERAV
                     DGKLWDVVLSEYEIPGFGALAALDVASRAALDAPVVVVFDSIDEDGARCLRRSGAVCF
                     PRTALSRLSIEVERTLSARRLRRERRQARQALQDSESRFRALAETGSDAILTTDTDGI
                     VIFANRATERVFGRPVPAVIGRPIEQLLPGILTRRDDDPGEREPSSGPELPIEWCGLH
                     ASGREIPVELSVSNFRRKGRRLLGVVARDITERRRAEDALKNSEARKSAVIEAAMDAI
                     ITIDAAGLVLEFNSAAERMFGHSRGAVIGRPMGDVVIPPAARERHREGMARYLATGKS
                     MILGKALEMTAMRADGSDFPIELMVTRLPSDGPPVFTGYVHDLSESRLAENALRESEE
                     RFRVAAQISTDLVYEWDLVGGKFRYLHARVPSPHNLPVSREEWEKLMHPEDRERVLAS
                     VQRTIDTDQAFFEEYRVILGDGTVRVRLGYGKVLRDRRGRALKWIGVSKDVTERRRTE
                     IALKESEQKLRTLVANIPVVLFAIDSDGTFTHSEGKGLETLGLKPSEVVGRPVWEVYR
                     KFPEVLEHVRRALSGDGHTATVRLGEVAFETRYAPYRDHEGRVVGVIGVATDVTEQQR
                     ADQALRASESRYRALFERNLAGVFRTTLDGEILDCNESFARIFGFSSPEEVLQQPALD
                     LYLHPGDRTTFVARIRERHAVSNHEVFARRRDGTPVWVLENATLVDGPDGPRTLIEGT
                     VIDITERKRAEEQVRHLAFHDALTGLPNRLLFNDRLAVALAQANRGHLKLAVFYLDLD
                     RFKVINDSLGHTVGDELLRRVGDRLRACMREEDTVARIGGDEFIILAPRVGSEQDATT
                     VARKILAAIRLPFAVEERDFFLTTSIGVSLYPTDGTDPEALVQNADTAMYRAKERGRD
                     NVQLSSPAITALAIDRLSLENRLRQALQNDELVLFYQPIVNLADGRVRGAEALVRWRH
                     PERGLLLPAEFIPVAEISGLIAPIGRWVLQTACSQIREWRAMGHPHFTVSVNLSPRQF
                     LQADLVSQVAGALSGSEIDPGCLDLEITESNAMENADLTINTLWELRRLGVGISMDDF
                     GTGYSSLNYLKRFPIDRVKLDQSFVRDVVANPEDAAIVRAVIAMAHTLRLIVAAEGVE
                     NEDQLSFLRQHQCDEMQGFLFSPPVAAAEFQSLLLRRQALPASA"
     gene            complement(8780..9325)
                     /locus_tag="VF958_04545"
     CDS             complement(8780..9325)
                     /locus_tag="VF958_04545"
                     /inference="COORDINATES: protein motif:HMM:NF012355.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="plastocyanin/azurin family copper-binding
                     protein"
                     /protein_id="HEX9148413.1"
                     /translation="MSFRGMIVGGMNRPRLLVAVLAALAPCAAAGDQMVQVGPGISFS
                     PATLTVAPGETVTWSFQAFHTSTSDSQTGPETWDSGLLSSGTFSHTFQTPGTYPYYCA
                     VHSYAGGTAMNGVVEVSGAGATATPTPTRTATPAPTSMPPTSPPPTAAGIPDLGAGGQ
                     LLLALTLAVVGVAALRLARRP"
     gene            complement(9420..10157)
                     /locus_tag="VF958_04550"
     CDS             complement(9420..10157)
                     /locus_tag="VF958_04550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9148414.1"
                     /translation="MPRRFWTTLFPRVLAGLCLLAALEGVASAQISPPGCNANNLNIT
                     VLKDKTAIVDPDTVTYTVSVTNGGTGACDVTGANVTFTCPGADGTPSGTVSNLGTGAS
                     FPADGSGDTTYAPIPCLVDVTPLVTTAIARASVSGQLHDSPNNTPFSINKDLGIDVAQ
                     PTPTPTNTPTNTPTNTPTNTPTNTPTTVATNTPTNTPTNTPTNTPTNTPTNPQTAPPP
                     VPTLSFPMMALLGLMLAGAGLFLARRQ"
     gene            10384..>11548
                     /locus_tag="VF958_04555"
     CDS             10384..>11548
                     /locus_tag="VF958_04555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011384850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit A"
                     /protein_id="HEX9148415.1"
                     /translation="MEFLRIRGARQHNLRNLDCDVPRNRLVVITGVSGSGKSSLAFDT
                     IFAEGQRRYVESLSAYARQFLEQMEKPDVDSIEGLSPAISIEQRTTSKNPRSTVGTVT
                     EIYDYLRLLYASVGKPHCPSCGRPIQAQTVQEMVDRALRLPEGSRFLILAPYVTGKKG
                     EYRKQMAEMVKEGFLRARIDGRLVELDDPPELDRQKKHTIELVVDRLVAKPARDEEYR
                     KRLADSIETATRVSGGLVVLSVADGPEEILSANYACPDCGTSLTEITPRLFSFNSPYG
                     ACPTCSGLGTILKADPERLVIAPEKSIQDGAIAILKPGSTNWRTRQMATLAKHYRFRL
                     DQPFSKIPKAAREVILYGSGEDEIRFSWVAEKGEYHWKSSFEGLVPMIERRYRE"
BASE COUNT         1934 a         3736 c         3982 g         1896 t
ORIGIN      
        1 tccggatcga gctgcacgcg gtcgcgcgag aggctggact cgacgcgcac ctggactccg
       61 tcgcgtactc cctcgaagag gcggacggcc gacccggcca ggccggcgag gtccaccgcc
      121 tcgcgcttca tctcgggaag acgcgcgaac cgggagaact cgtccacgag ggacttcagg
      181 acgccgacct catcgacgat gacccgcgtg ccccgctcga tcgcttccgg gaggtcgggc
      241 tctccggccc ggagccggcg gaggatgcgc tcggcggaaa ggcgaatcgg ggtgaggggg
      301 ttcttgatct cgtgggcgac ccgccgcgcc acttcgctcc aggccgccag cttctgctcc
      361 cgggcgacgt gcgtcgtgtc ctcgaccgcg acgacccagc cgccgcgatt gcccggcgac
      421 gccgacgaca tcgggcgcag cgcgatctcc agccggcgct cgcccccgtc gcccgagagg
      481 atgagctccc gcggagtttc cggcgccgct ccctcctccg ccttttcgag aaggcggacg
      541 aggggcgcga gctcgggccg cccacgcagt ccctcgaggg tgacctcggc gtctccgaga
      601 cggaggagcg atcgggcggc gggattcgcc accgtggcgc ggccgctcgc agcgaacgcg
      661 agaaccccgg tcgtgaccga tcccagaacc gtggagcgga gacctcgttc gaggtcgagc
      721 ctccggttga tttcctggag ctcctcgttg ctgcggagcg tcgcgtcgcg cgcctcctgg
      781 agacctgccg ccatgctgtt gaacgagtcg acgagaacgt cgagctcgtc ggctgcggcg
      841 acgtccacgc gcgccgtgaa gtccccggcc cggatccggc gcgtcgactc tgcgagcgcc
      901 gcgatgggtc ccgtgatccg ccgggcgagc gtcatgcccg tccagatcga ggcgagcagg
      961 accgcgaggg tgatgagcag gaaggtcgag acgttcgctg ccttgatcgc gggcttctga
     1021 acctcgagct tctggtagtc gctccaggcg gaggcgatcg cccgcatcgc acgggcgtcg
     1081 gagggaggca ctcgaactcc cgccgcgaag acgcgggttc ccgaccgtct cgccgcccgg
     1141 aagaggtgcg agccgtcggc aaggacctcg accttgagag cctcgccgcg cgagaacgcc
     1201 gtctcgagag tttcggcggg gagcggggcg aggtccccga agtccgccgc cgttccctcg
     1261 cgcgcggagc cgcgatagac ctcgacggag tcgagccttt cctgtcggcg gaattcctcg
     1321 agactctccg ccaggcggtc ggccgaaact ccctcgacgg ggaggcgcga gagagacttt
     1381 gcgtcctcga cggcggcgtc cgtgatcctt ctttccgcca tgtcctcgac ggaacgggcg
     1441 cgcccgacga cttcccggac cggcgaggag aaccaacggt cgatcgagcg ctgcaggaat
     1501 ccggtcgcag tgaagaagag cagtccgatg gggatcgcgg tcaggccgat gtacgtcacg
     1561 aggagccgtg tcttgaaccg cgaacccaga acgccccgcc gttgttcgag ccacaccttg
     1621 atgagggtcc ggcagagcac gaagaggacc gcggcgatca gcgtgacgtt caggtaggag
     1681 agaacgaaga ggagtagccc gcgtgtgatg gtcgccggct ccatttcctc ggcccgctgg
     1741 cggagcaccg aggaggcgga ccacaccacg agcacggcga gcgccaggaa gacgacgagt
     1801 cggttctctt tgcggtgccg ctggagctcc gcgacgaggg tcggaacacg cttttctccg
     1861 cgtggagtct gcggcggttt ttcgtcgctc acggcgtcac cgcgcggatg ttgaacctct
     1921 tcgacttctt ccacggggtg cgcgaatcga ccggaatgat cgtgaagacg tatcccgcgt
     1981 cgaagctcgc gcgcatctgg acgtagaggt tccgggtcgg gcgcccccgc tggatgtgga
     2041 aggcgagaag gttttcccgg gagagaagaa gcttcgccgc ctccggaaaa tcgcgagtcg
     2101 tgttgcggcc gagcggcttg ccgtcgagcg tctcgtcgac gccatactcc ctcgtcacgg
     2161 gatcgaacgt caccgcgaca cggtatttcc tcctctcgac gaggtcatcg aaccagtagg
     2221 ggtaccgacg atagaggcgg atgtcgtagc ggatggcggt ctcgagcccg gcctcgattt
     2281 tgcgcgcgag ctcggggttc aaggcgcccg ccagggcgaa cgtgacgaag acgtcgcggc
     2341 cgcgcgactc gacccgcagc tcgcggacgc ggggaccgct gtccgtctgg ccggaggcgg
     2401 ggcctcccgc aaacacgctg ccgacgagga ccgcggcgat cgtgcgacga agcatcacct
     2461 cgggattcta ggtccaaggt ccaacgtcga acgtccgacc cgggagaacc ggacggactc
     2521 cggcccacga aaacggcggt cccggggttt ccggctagcg cgagactcgg cggcggggac
     2581 gctccacggc aacgtccttc gcctggcgcc ggccgtggga ggcggcggcg agtgcccgcg
     2641 cgaactcgat gtgcagcgcg tgaccggccc ggcgcgcgtg gatatggcca tggagcgaga
     2701 cgcccaggag ggccaggtcg cccacgaggt cgagaatctt gtgtcgaacg aattcgtcgc
     2761 ggaaacggag gggcccggag aggacgccct cctcgtcgag gacgacggcg ttgtggaggc
     2821 tcccgccgcg ggcgaggccc ctcgagcgga gagcatcgac gtctttggcg aagccgaacg
     2881 tgcgagcggg ggcgatccgt gtcgaatact cctccgggcg gatcgcgacg gtcagctcct
     2941 ggcggcccac cacgggatgc ggaaaatcga tctcgtaggt gatcgtgagg cccggaccgt
     3001 ccggctggaa cgaaacgccg cgcccgccgc gggaaacctc gaccggcccg gaagaagcga
     3061 gcgattcgac gggggagtcc aaacgctcga atccggcggc gtgaaagagg cgcacgaaag
     3121 ggagggccga tccatcgagg atcggaacct ccggcccgtc cagctcgatc acgcagttgt
     3181 cgagtccttc gccgtaggcg gccgagagga cgtgctcgac cgtgccgact gagacgccgt
     3241 cgacgctcaa cgtcgtcgcg tagtcgaacg tgctcgccga ctcgacccgc gctggaacca
     3301 gcgtgtcggg ccggtccaga cggcggaaga cgatcccgtg acccgccgga gccgggcgaa
     3361 acgacgcgcg aacagggtgc ccggagtgaa gtccgacacc ctcgatcgag acgcttgttc
     3421 gaagtgttgt ttgtgcgcga gccacggtga actcgggccc ctaccgcacc gatgatgcca
     3481 gaccggagcc cgtcgagcag atttctcgca tctccttgtt tctctggagt ttacggatcc
     3541 gagtcccatt tcgtcacggg gcaaagccgt ggcggcatcg atacagatgc gtggcagggc
     3601 tgggacacgg gggcgcggcc gaattgggcc gataatcgcc ggtccctcgg cggagacggt
     3661 gcgccgctgt ggcgaggtcg agtctgcgga aggtggtcga gagattgaag acgaagcaaa
     3721 gaacagccga cgacggcgtc aacacggttg cgacgaaccg caaggcgttc cacgactact
     3781 tcatcgagga caggaccgag gcggggctcg ttctgaccgg gaccgaggtc aagtcgctgc
     3841 gtgaagggcg tgcgcagctg aaggactcct acgtccagtt tcgcgcgggc gaggcgtatc
     3901 tgatcggcgc acatatctcc ccttacagcg cgggatcgtg gtcgaatcac cttccggaga
     3961 gggagcggaa gctgctcctg caccggcgcg agatcgagag gtgggcggcc gccgtggcca
     4021 cgcgcggagt cgcctgcgtt ccgctgtcgc tctacttcaa ggggccccgc gcgaaggcgc
     4081 agatcgcgct cgtccgcggg aagaaacagc acgacaaaag agacgcgatc cgcgagagag
     4141 atctcaagcg cgaggcggat cgcgcgatgc gcgagcggtc gcgctgattc aggccggctg
     4201 gacgtcgacc ttgtccccga cccgcagcca tcccttcgtc aggatctgcg cgcgcaggcc
     4261 gccgcgatgc acgagtcctt cacgcacggg tttaccgcac tttcgttcca ggtagccgca
     4321 cggctcgcag agccgaagcc ccaggaggac cgtctccccg actcgaaacc gcgccccgac
     4381 gaggtgattg agcgccactc cgcgcgtcgc gacgttgcgg cgggtctcgt tcggctcgag
     4441 ccggatgccg tagtcgcgct ccagcgcctc gaccgcttcg atctcgatca gcgtgatctc
     4501 ccggtccggc ccgtcccggt gcgaataggt accgctcttc gagaagtacc ggtcgccttc
     4561 gaggccccgg ccggggaccg cgtgggcgga atcgaccgag ctcatcggcg cgccggcgga
     4621 gcccgcgacg tgaatcgatt caacacgacc ggcccgcatc aggaccgcag tctaaactcc
     4681 ttccatgatc cgacgagcgc gtcccggaga cctgcgccgc ctgtgggagc tcgaccggat
     4741 ctgcttcgag ccgggaatcg cgtactcccg tgcggagctg cggaggtttc tcgacctccc
     4801 gcgtgcaaag tgtctggtcg cggagagtgc gggagcgatc gacggcttcg cggtcgggta
     4861 tcccgacccg ccggacctcg cacgcgtcgt cacgctcgac attcatccct ctgctcgccg
     4921 gcgcggcctc gggaaggagc tgctcgagcg cctgctggcc gagctcggcg aggcgggcgg
     4981 cggacgcgca gtgctcgaag tggacgttcg caatacggga gcgatcgcct tctaccgccg
     5041 actcggattt tcgacgatgc gccggattcc cgactactac ggaccggggc tcgacgcttt
     5101 cgagatggta cgaggccccg gcgtcacgcg ctcgccggaa gcgcctggcg gcggagcaga
     5161 aggctctgaa attcggcggc cgccacgggg ggactgaaga ggaacccctg catttcgtcg
     5221 cactgatgct ggcgcagaaa cgagagctga tcctcgttct cgaccccctc cgccgccacg
     5281 atgagacgga gggtgtgcgc catcgcgatg acggcgcgga cgatggctgc gtcttccggg
     5341 ttcgcgacga cgtctctcac gaacgactgg tcgagcttga cgcggtcgat cggaaacctc
     5401 ttcagatagt tgagcgagga gtatccggtt ccgaagtcgt ccatcgagat cccgacgccg
     5461 agcctccgca actcccagag cgtgttgatc gtcaggtcgg cgttttccat cgcattcgac
     5521 tcggtgattt cgagatcgag gcagcccgga tcgatctcgc tcccgctgag cgctcccgcg
     5581 acctgcgata cgaggtctgc ctggaggaac tggcgcggcg agagattgac cgagacggtg
     5641 aagtgcggat ggcccatggc ccgccactcc cggatctgcg agcaggcggt ctgcaggacc
     5701 cagcggccga tcggcgcaat gaggccggag atctccgcaa cggggatgaa ttccgccggc
     5761 aacagcagac ctcgctcggg atgtctccat cgaacgagtg cctccgctcc ccgcacgcgg
     5821 ccgtccgcga ggttgacgat cggctggtag aagaggacga gctcgtcgtt ctggagcgcc
     5881 tggcgtaagc ggttctcgag cgaaagacgg tcgatcgcga gcgcggttat cgcaggcgag
     5941 gagagctgga cgttgtcgcg gccgcgctct ttggcccggt acatcgccgt atcggcgttc
     6001 tggacgagcg cttcgggatc ggttccgtcg gtcgggtaga ggctgactcc gatcgacgtc
     6061 gtgaggaaaa agtccctctc ctcgacggcg aaagggagcc ggatcgcggc gaggatcttg
     6121 cgggccactg tcgtcgcgtc ctgctcggaa ccgactctcg gcgcgaggat gatgaattcg
     6181 tcgccgccga tcctggcgac ggtgtcttcc tcccgcatgc atgcgcggag ccggtcgccc
     6241 acgcgccgca gcagctcgtc gccgaccgtg tgaccgagcg agtcgttgat gaccttgaag
     6301 cggtcgaggt ccaggtagaa gaccgcgagc ttcaagtgac cgcgattggc ctgcgccagc
     6361 gcgacggcca gacggtcgtt gaaaagcagc cggttcggga ggcccgtcag ggcgtcgtga
     6421 aacgcgaggt gccgcacctg ctcctccgca cgctttcgct ccgtgatgtc gatgaccgtg
     6481 ccttcgatca aggtccgcgg gccgtcaggc ccatcgacga gcgtcgcgtt ctcgaggacc
     6541 cacacgggcg tcccatcccg tcggcgcgcg aacacttcgt ggttgctcac cgcgtggcgc
     6601 tcgcggatgc gcgcgacgaa ggtcgtgcgg tcgccggggt gaaggtacaa gtcgagcgcc
     6661 ggctgctgga ggacttcttc cggcgacgag aagccgaaga tgcgcgcgaa ggactcgttg
     6721 cagtcgagga tctctccgtc gagcgtcgtt cgaaagactc cggcgaggtt tcgctcgaag
     6781 agcgcccggt accgcgactc ggaggcgcgc agcgcctggt cggcgcgttg ctgctccgtg
     6841 acgtcggtag cgacgccgat gacgcccacg acgcgaccct cgtggtcccg gtagggcgcg
     6901 tagcgggtct cgaacgcgac ctctcccagc cggacggtcg cggtgtgccc atctccggag
     6961 agggcccgac ggacgtgctc gaggacctcc ggaaacttcc gatagacctc ccagaccggg
     7021 cggcccacga cctcgctggg cttcaggccg agcgtttcga gccccttgcc ctcggagtgg
     7081 gtgaaagtgc cgtcgctgtc gatcgcgaaa agaacaaccg gaatgtttgc aacgagtgtc
     7141 ctcagcttct gttcgctctc cttgagcgca atctccgtcc ggcgccgctc cgtgacgtcc
     7201 ttgctcacgc cgatccactt gagcgctcga ccgcgtcggt cccggaggac cttgccgtat
     7261 cccaaccgca cgcgaaccgt tccgtcgccc aggatgacgc ggtattcctc gaagaaagcc
     7321 tgatcggtgt cgatcgtacg ttggacggac gccagcacgc gctcgcggtc ctccgggtgc
     7381 atgagctttt cccactcttc gcgcgaaacc ggcaggttgt gggggctcgg aacgcgcgcg
     7441 tgcagatagc ggaatttccc gccgacgagg tcccactcgt agacgaggtc ggtcgagatc
     7501 tgcgcggcga cgcggaaccg ctcctccgat tcccggaggg cgttttcggc aagtctgctc
     7561 tccgagaggt cgtgcacgta tcccgtgaag accggcggac catccgaggg aagccgtgtg
     7621 accatcagtt cgatcggaaa atctgagccg tcggcgcgca tcgccgtcat ctcgagtgcc
     7681 ttgccgagga tcatggactt ccccgtcgcg aggtagcgcg ccattccctc gcggtgccgc
     7741 tctcgcgcgg cgggtgggat gaccacgtcg cccatcggcc gtccgatgac ggcgccccgc
     7801 gaatggccaa acatccgttc ggcggcggaa ttgaactcga ggacgagccc ggccgcgtcg
     7861 atcgtgatga tcgcatccat tgccgcctcg atgacggcac tcttgcgcgc ttcggagttc
     7921 ttcaaggcgt cttcggctcg ccttcgttcg gtgatgtccc gcgcgactac accgagtagc
     7981 cgtctccctt tccgccggaa gttggagacc gacagttcga cgggaatctc ccgaccgctt
     8041 gcgtggagac cgcaccactc gatcggaagt tcgggcccgg aggagggttc gcgctctccg
     8101 gggtcatcgt cgcgacgcgt caggatgccg gggagcagct gctcgatcgg ccggccgatc
     8161 acggcgggga ctggacggcc gaaaacgcgt tccgtcgcgc ggttggcgaa gatcacgatt
     8221 ccgtcggtgt cggtcgtgag gatcgcgtcg gatccagtct cggcgagcgc gcggaaccgg
     8281 gactcgctgt cctgcagagc ctggcgggcc tggcgccgct cgcgccgaag ccgcctcgcg
     8341 gaaagggtcc gctcgacttc gatcgacagc cgtgacagcg ccgtgcgcgg gaagcacaca
     8401 gcgccggagc gtcgcaggca gcgagcgccg tcttcgtcga tcgaatcgaa aaccacgacg
     8461 acgggcgcgt cgagagcggc gcgggaggca acgtcgagcg ctgcgagtgc gccgaaccct
     8521 ggaatctcat actccgacag cacgacgtcc cagagcttcc cgtcgaccgc gcgctccatc
     8581 tccgagccgt cgcggacgac ctcccaggcg acttcgtatc cggcgcgcgt gagctcgtcg
     8641 acgagaggct cggcgtcgcc gggagaggcc gcgacgagca gaacccgcag cgacatttcc
     8701 accaatctct tcggttgttg tttgcgtaag tatagcgtca gcgcggtcgt gatccgcgtc
     8761 acgcggcgcc actccggact caggggcgtc gtgcgagccg gagggcggcg acgccgacga
     8821 cggcgagtgt caatgcgaga aggagctggc ctcctgcgcc gagatcgggg attcccgcgg
     8881 cggtcggtgg cgggcttgtc ggcggcatcg aggtcggcgc cggcgtcgcc gtccgagtcg
     8941 gtgtgggagt cgccgtcgcg cccgcgccgc tgacctcgac tacgccgttc atggccgtcc
     9001 cacccgcgta gctgtggacg gcgcaatagt acggatacgt gccgggcgtc tggaacgtgt
     9061 gcgagaaggt gccgctcgaa agaagaccgg aatcccaggt ttccggtccc gtctgtgaat
     9121 cgctcgtcga cgtgtgaaag gcctggaagg accaggtgac cgtctctccg ggcgcgacgg
     9181 tgagggtggc tggtgagaac gaaatgcccg gcccgacctg gaccatctga tcacctgccg
     9241 ccgcgcaggg agcgagggca gcgagtacgg cgacgagcag ccgggggcga ttcatcccgc
     9301 ccacgatcat ccccctgaag gacatgcgcg gcgtcttcac gaatctctaa ggaaaaaaat
     9361 gaagcaaaaa atccgggacc ctttcgggtc ccggatcatg gagcgctttc gaggacgggt
     9421 tactgacggc gcgcgaggaa caggcccgct ccggcgagca tcaatccgag cagagccatc
     9481 atcgggaagg aaagcgtcgg aacgggaggc ggcgccgttt gagggttcgt cggcgtgttg
     9541 gtcggcgtgt tggtcggcgt gttcgtcggc gtgttggtcg gcgtgttggt cgcgacggtg
     9601 gtgggcgtgt tcgtgggggt gttggtcggc gtgttagtcg gcgtgttggt cggcgtattc
     9661 gtgggcgtcg gggtcggctg cgctacgtcg atcccgaggt ccttgttgat ggagaacggc
     9721 gtgttgttcg ggctgtcgtg gagttggccc gaaaccgagg cgcgcgcaat ggccgtggtc
     9781 accagcggag tgacgtcgac caagcacgga atcggcgcgt aggtcgtgtc gccgctgccg
     9841 tccgccggaa aacttgctcc ggtcccgaga tttgacaccg ttcccgacgg ggtgccgtcg
     9901 gcgccggggc aggtgaacgt gacgtttgcg ccagtcacgt cgcacgcgcc cgttcccccg
     9961 ttcgtgacgg acacggtgta ggtcaccgtg tcgggatcca cgatggccgt cttgtccttg
    10021 aggaccgtga tgttcaggtt gttcgcgttg cacccgggcg gggaaatctg cgcactcgca
    10081 accccctcca acgccgcgag gagacagagc ccggcgagaa cccgagggaa gagcgtagtc
    10141 cagaaccgtc tcggcatttt gttctccgta acttgtggga gttccttggg ttcgaggagc
    10201 ttatcccctc atgacataag tctaagggtg tccaatctcc gagtcaacgg aagacttgta
    10261 acctcattca cccgcgccgg gaggcggcgt atccctagct cctctctggg gttatcgggc
    10321 gccgggacgg tccgtgcggc cgccgccacg ggtcgtgacg cggcggctac aatccgctct
    10381 cccatggaat ttctgcggat ccgcggcgcc cgtcagcaca acctgcgcaa cctcgactgc
    10441 gatgtccctc gcaaccggct ggtcgtgatc accggagtct ccggatcggg caagtcctcg
    10501 ctcgccttcg acacaatttt tgcggagggg cagcggcgct acgtcgagtc gctgtcggcc
    10561 tacgcccggc agtttctcga gcagatggag aagccggacg tcgattcgat cgagggtctc
    10621 tccccggcaa tatcgatcga gcagcgcacg acgtcgaaga acccgcgctc gaccgtgggg
    10681 acggtcaccg agatctacga ctacctgcgc ctcctttacg cgtcggtcgg caagcctcac
    10741 tgcccgagct gcggccgacc gatccaggcg cagaccgtcc aggagatggt cgaccgcgcg
    10801 ctccggcttc ccgaaggcag ccgcttcctg atcctggccc cgtacgtgac ggggaagaag
    10861 ggtgagtacc gcaagcagat ggcggagatg gtcaaggagg gtttcctccg agcccgaatc
    10921 gacgggcggc tcgtcgagct cgacgatcct ccggagctcg atcggcagaa gaaacacacg
    10981 atcgaactgg tcgtcgaccg cctcgttgca aaaccggccc gcgacgagga ataccgcaag
    11041 cgcctggccg attcgatcga gacggcgacg cgggtgtccg gcggactcgt cgtgctgtcc
    11101 gtcgccgacg gtccggaaga gattctctcc gcgaactacg cctgtccgga ctgcggcacc
    11161 tctttgacgg agatcacgcc ccgcctcttt tccttcaact caccgtacgg ggcgtgtccg
    11221 acgtgctccg gcctgggaac aatcctcaag gcggacccgg aaaggctcgt gatcgcgccg
    11281 gagaaatcga tccaggacgg cgcgatcgcg atcctgaagc ccggctcgac caactggcgc
    11341 acgcggcaga tggccaccct cgccaagcac taccgcttcc gcctggacca gcccttctcg
    11401 aagatcccga aagcggcccg ggaagtcatc ctctacggat cgggggaaga cgagatccgc
    11461 ttctcgtggg tggccgagaa gggcgagtac cactggaagt cctcgttcga gggcctggtg
    11521 ccgatgatcg agcgccgcta tcgcgaga
//
LOCUS       DASWAV010000225         2816 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U2665
            k141_531848, whole genome shotgun sequence.
ACCESSION   DASWAV010000225 DASWAV010000000
VERSION     DASWAV010000225.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960316
            Sequence Read Archive: SRR10260007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2816)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2816)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:47:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,929
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2816
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_531848"
                     /isolate="SMAG_U2665"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.36444 N 132.31889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(62..880)
                     /locus_tag="VF958_04560"
     CDS             complement(62..880)
                     /locus_tag="VF958_04560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016436021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX9148416.1"
                     /translation="MGTLNRKTLFITGGSRGIGLAIALRAAADGANVVVAAKTAEANP
                     KLPGTIFTAAREIEQAGGKALPLRVDIRFEEQLAAAAEKAAESFGGIDILVNNASAIS
                     LTGTLQTPMKRFDLMFGVNVRGTFAASQALLPYLLKAKNPHILTLSPPLSLDPKWFGN
                     HCAYTMAKYGMSMCVLGMAEEFRGAGVAVNALWPRTVIATAALAMIPGVEMKNCRKPE
                     IVADAAHWILTLNRRACTGNFFLDDEVLASEGVTDLSRYAVEPGAPLLPDLFLA"
     gene            1030..2019
                     /locus_tag="VF958_04565"
     CDS             1030..2019
                     /locus_tag="VF958_04565"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012330591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEX9148417.1"
                     /translation="MERRGLGRSGLTVSAMGLGCMGMSEFYGPADEAESLATIHRAID
                     LGIDLLDTSDIYGLGHNETLVGKAIRDRRDRVVLATKFGLLRGEDGSWKGVNGKPAYV
                     RSACEASLTRLGVDVIDLYYLHRVDPDTPIEETVGAMAELVREGKVRFLGLSESAAGT
                     IRRANGVHPIAAVQSEYSLWTRDPEENGALAACRALGIGFVPFSPLGRGFFAGRVKSK
                     ADLFEKDFRRSSPRFEGGNLERNVELLRAFEAVARDKGCTPAQLALAWVLSRGKDVVP
                     IPGTKRRERLEENVAALTVSLSKGDIARIEAAVPPSAVAGMRYPAESMQAVNR"
     gene            2029..2526
                     /gene="ybaK"
                     /locus_tag="VF958_04570"
     CDS             2029..2526
                     /gene="ybaK"
                     /locus_tag="VF958_04570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010943709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-tRNA(Pro) deacylase"
                     /protein_id="HEX9148418.1"
                     /translation="MSKEKMPVTAAIRVLRAAGAAYTEHPYGYEEKGGTAVSARELGV
                     DEHSVVKTLVMEDDRKRPLIVLMHGDREVSTKELARQIGAREVAPASPAAVEKHTGYV
                     PGGVSPFGTRARLPVYVEETVLALDRFLINGGRRGFLVEIAPADLERVLAPVPVRVAI
                     ESRHG"
     gene            2519..>2816
                     /locus_tag="VF958_04575"
     CDS             2519..>2816
                     /locus_tag="VF958_04575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018004322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX9148419.1"
                     /translation="MVEIAAAGAADRPAVVRLVARLLAELEDSPSEFAGLDEGGALRA
                     LETDGGAFHAFLAREGGEPVGVLTLTESFAIYAGGRYGVIDELYVAPGHRSSGIG"
BASE COUNT          433 a          911 c         1030 g          442 t
ORIGIN      
        1 aattacacca tcgatcgctg ccgaccggtt tgacccacgt caatcgcgcg aggaattcgc
       61 ctcaggcgag gaagaggtcg ggaaggagcg gagcgcccgg ctccacggcg tagcgggaga
      121 ggtccgtcac gccttcggag gcgagcacct cgtcgtcgag gaagaaattg ccggtgcacg
      181 cgcggcggtt gagcgtgagg atccagtgtg ccgcgtcggc gacgatctcg ggcttgcggc
      241 aattcttcat ctcgacgccg gggatcatcg cgagggccgc ggtggcgatc accgtccgcg
      301 gccacagggc gttcaccgcg acgcccgcac cccggaactc ttccgccatc ccgaggacgc
      361 acatgctcat cccgtacttc gccatggtgt aggcgcaatg gttcccgaac catttcggat
      421 ccagggagag gggcgggctc agggtgagga tatgtgggtt tttcgccttc aggaggtacg
      481 gcaggagcgc ctgcgacgcg gcgaaggttc cccggacgtt cactccgaac atcaggtcga
      541 accgcttcat cggggtctgc agcgtccccg tcaggctgat cgcgctcgcg ttgttcacca
      601 ggatgtcgat gccgccgaac gactccgcgg ccttctcggc cgccgccgcg agctgctcct
      661 cgaaccggat gtcgacccgg agcgggaggg cctttcctcc cgcctgttcg atctcccggg
      721 cggccgtgaa gatggtgccg ggaagcttcg ggttcgcttc cgccgtcttg gcggcgacga
      781 ccacgttcgc gccgtccgcc gccgcacgca gggcgatcgc caggccgatc ccccggcttc
      841 ctcccgtgat gaagagcgtc ttcctgttca atgtgcccat gtcggagcct ccggcccgtt
      901 cgattcgtac gcctaccgta tgacacgcgt cgtttccggt caaccgggac cgccctgcgg
      961 atctccgggg tagaatggag ctgtcccccg gatgccgatc ggggcgatca tgtacgtgga
     1021 ggtgaaggga tggaacgcag ggggctaggc cgcagcggtc tcacggtgtc ggcgatgggg
     1081 ctcggctgca tggggatgtc ggagttctac ggtcccgccg acgaggcgga gtcgctcgcc
     1141 acgatccacc gcgcgatcga cctcgggatc gacctcctcg acacgagcga catttacggt
     1201 ctcgggcaca acgagaccct ggtcggcaag gcgatccggg atcggcgcga ccgggtcgtc
     1261 ctcgcgacga agttcgggct gctccgcggg gaggacggga gctggaaggg agtgaacggg
     1321 aaacccgcgt atgtgcgctc cgcctgcgag gcgagcctta cgcggctcgg cgtggacgtg
     1381 atcgacctct actacctgca ccgcgtggac cccgacacac cgatcgaaga aaccgtgggc
     1441 gcgatggcgg aactcgtccg tgaagggaag gtccggttcc tcggactttc cgagtcggcg
     1501 gccggcacga tccggcgggc gaacggtgtc cacccgatcg ccgcggtcca gagcgagtac
     1561 tccctctgga cccgggaccc ggaggagaac ggggcgctcg ccgcgtgccg cgcgctgggg
     1621 atcggattcg tccccttctc cccgctgggg cgcggcttct tcgccgggag ggtgaagagc
     1681 aaggcggacc tgttcgagaa agacttccgt cgttcctcgc cgcgattcga gggggggaac
     1741 ctcgaacgga acgtggagct gctgcgggcg ttcgaggcgg tcgcgcggga caaggggtgc
     1801 acacccgcgc agctcgctct ggcctgggta ctgtcgcgcg gcaaggacgt cgttccgatc
     1861 ccggggacga agcggcgcga acgcctggaa gagaacgttg ccgcgcttac ggtctccttg
     1921 tcgaaagggg acatcgcgcg gatcgaggcg gcggtcccgc cgtcggcggt ggcggggatg
     1981 cggtatcctg ccgagtcgat gcaggcggtc aaccgctgaa gcgctgagat gtcgaaggag
     2041 aagatgccgg tgacggccgc gatccgcgtc ctgcgggcgg ccggagcggc gtataccgag
     2101 cacccgtacg gctacgagga gaagggcggg acggccgtgt ccgcgaggga actcggggtc
     2161 gatgagcaca gcgtggtgaa aacgctcgtg atggaggatg accggaagcg gccgctgatc
     2221 gttttgatgc acggcgaccg cgaggtgtcg acgaaggagc tcgcacggca gatcggcgcc
     2281 cgggaggtcg cacccgcaag cccggcggcg gtcgagaagc acaccggcta cgttccgggc
     2341 ggcgtcagcc cgttcggcac gcgcgcgcgg ctgcccgtct acgtcgagga gacggtgctc
     2401 gcgctcgacc gattcctgat caacggggga cggcgcgggt tcctcgtcga gatcgccccg
     2461 gccgacctcg agcgggtgct cgcgccggtc ccggtccggg tcgcgatcga gagccgccat
     2521 ggttgagatc gccgccgccg gggcggccga ccggcccgcg gtcgtgcgcc tggtcgcgcg
     2581 gctgctcgcc gagctcgagg attcgccctc cgagttcgcg ggcctcgacg agggcggggc
     2641 gctccgcgcg ctcgagaccg acggcggcgc gttccacgca ttcctcgcgc gcgagggcgg
     2701 cgaaccggtc ggagtgctga cgctgaccga gagcttcgcg atctacgccg gcggacgcta
     2761 cggcgtcatc gacgagctct atgtcgcgcc cgggcaccgg tcgagcggca tcggcc
//
LOCUS       DASWAV010000226         1504 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U2665
            k141_252537, whole genome shotgun sequence.
ACCESSION   DASWAV010000226 DASWAV010000000
VERSION     DASWAV010000226.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960316
            Sequence Read Archive: SRR10260007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 1504)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1504)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:47:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,929
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1504
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_252537"
                     /isolate="SMAG_U2665"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.36444 N 132.31889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1404
                     /locus_tag="VF958_04580"
     CDS             <1..1404
                     /locus_tag="VF958_04580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008766152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase C-terminal domain-containing
                     protein"
                     /protein_id="HEX9148420.1"
                     /translation="RIVHCDGLDVFDSFRAMREAVDYVRTGQGCAIVYAPCVRIHSHS
                     NSDRHELYRSPEELEAAREADPLPRFRRTLIDGGLLSEEEIVAIEAHNQQTYEEAADR
                     ARRAPDPTPESIFEYVTPEPWTPDGWPEGIPDGTGEAMTLVQSINQTLKEEFRRNPDT
                     YIWGQDVAYRDKGGVFNVTKGMQQEFGPRRVFNGPIAEDFILGTSDGFSRLSEDIRVV
                     VEGAEFADYFWPAAEQMVEMSHEYWRTNGKFVPNVTIRIASGGYIGGGLYHSQNIEAW
                     LTTLPGLRVVYPAFADDAAGLLRTSMRSRGLTLFLEPKFLYNNPMARAAVPQEFAVPF
                     GKARVRREGSDLTIVAYGTPVHFALEAAAELEKEGKGVEVLDLRSLVPLDLDAISRSV
                     RKTTRALVAHEDKVHGGFGGEVVSQIQETVFPFLDAPIGRVGSTFTPVGFNRILERAV
                     LPSTEKVLAVARKVLAY"
BASE COUNT          248 a          485 c          513 g          258 t
ORIGIN      
        1 cggattgtgc actgcgacgg gctcgatgtc ttcgactcct tccgcgcgat gcgcgaggcc
       61 gttgactacg ttcggaccgg gcagggatgc gcgatcgtct acgcgccgtg cgtgcggatc
      121 cactcgcact cgaactcgga tcgccacgag ctctatcggt cacccgagga gctggaggcg
      181 gcccgcgaag ccgatccgct gccgcgcttc cggcggacgc tcatcgacgg cgggctcctc
      241 tcggaggagg aaatcgtcgc gatcgaggcc cacaaccagc agacctacga ggaggcggcc
      301 gaccgggcgc ggcgagcgcc ggatccgacg ccggagtcga tcttcgagta cgtgacgccc
      361 gagccgtgga cgcccgacgg ttggccggag ggaatccccg acgggacagg cgaagcgatg
      421 actctcgtgc agtccatcaa ccagacgttg aaggaggagt tccgccggaa tccggatact
      481 tacatctggg gacaggacgt cgcctatcgc gacaagggcg gcgtcttcaa cgtgaccaag
      541 ggaatgcagc aggagttcgg gccgcggagg gtattcaatg gcccgatcgc cgaggacttc
      601 atcctgggca cttccgacgg gttctcgcgc ctttccgagg acattcgagt cgttgtcgag
      661 ggcgcggagt tcgccgacta cttctggccg gcggcggagc agatggtcga gatgtcgcac
      721 gagtactggc ggacgaacgg aaagttcgtg ccgaacgtga ccatccgcat cgcctcgggg
      781 ggatacatcg ggggcggcct ctatcactcg cagaacatcg aagcgtggct gacgacgctc
      841 cccggcctcc gggtcgtgta tccggctttc gccgacgacg ccgcaggcct gctccgcacg
      901 tcgatgcgct cgcgcggcct cacgcttttc ctcgagccga agttcctcta caacaacccg
      961 atggcgcggg cggctgtgcc gcaggagttc gccgtgccgt tcgggaaggc gcgtgtgcgc
     1021 cgggaaggat cggacctgac catcgtcgcg tacggaacgc cggtccactt cgcactggag
     1081 gctgcggccg agctcgagaa agagggaaag ggcgtcgaag tcctcgacct gcgctcgctc
     1141 gtaccgctcg acctcgatgc gatctcgcgg tccgtccgca agacgacgcg cgctctcgtc
     1201 gcgcacgaag acaaggtgca cggcggcttc ggcggcgagg tcgtctcgca gatccaggag
     1261 accgtgttcc ccttcctcga cgcgccgatc ggacgggtcg gctcgacctt cactccggtc
     1321 ggcttcaacc ggattctcga gcgcgcggtc ctgccgtcga ccgagaaggt cctcgctgtc
     1381 gcgcggaagg ttttggccta ctgatcacgt ttcccggttc gaatgtctgt ggcagggctc
     1441 ggaccggacg atggagaggc cgcttctccc ttcggggcgg gtccttcggc gaaactcgtc
     1501 gcgt
//
LOCUS       DASWAV010000227         1509 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U2665
            k141_452724, whole genome shotgun sequence.
ACCESSION   DASWAV010000227 DASWAV010000000
VERSION     DASWAV010000227.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960316
            Sequence Read Archive: SRR10260007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 1509)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1509)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:47:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,929
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1509
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_452724"
                     /isolate="SMAG_U2665"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.36444 N 132.31889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..431
                     /locus_tag="VF958_04585"
     CDS             <1..431
                     /locus_tag="VF958_04585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF5009 domain-containing protein"
                     /protein_id="HEX9148421.1"
                     /translation="LVGGFFVLAVGSLWGRVFPINKNLWTSSYSVFMSGLAAVVLAIC
                     LWIVDVHGWKGWGRPFEWLGRNAIASFVVSTLATILMLAVKLSGPEGKPRSLYATIYR
                     VVFDRFADARLGSLLFSLSYLAIWLAVFGVLYRKRVFVKI"
     gene            complement(477..1265)
                     /locus_tag="VF958_04590"
     CDS             complement(477..1265)
                     /locus_tag="VF958_04590"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX9148422.1"
                     /translation="MGERVKHEPGKFCWVELATKDGPAAKKFYTSLFDWRVNELPTGD
                     GATYSMLEKDHKEAGALYELTPQQKGTPPHWNSYIRVASADDAAAKSRQLGGKILTEP
                     FDVMDQGRMANIQDPTGATFAVWEAKKHTGAEVVNEPGAFCWNELYTPDPKKAGDFYA
                     RLFGWTRETMPLPTGQEYTIFKKGDAQAAGMMELPSEWKVPPHWLVYFAVDDSDRTVS
                     KASGMGAKVMMPPTDIPNVGRFAILNDPQGADFAVIHMETPSQS"
BASE COUNT          230 a          482 c          469 g          328 t
ORIGIN      
        1 tactcgtcgg cggcttcttc gtgctcgcgg tcggatcgct ctgggggcgc gtgttcccga
       61 tcaacaagaa cctgtggacc tcgtcgtact ccgtcttcat gagcggcctc gcggcggtcg
      121 ttctcgcgat ctgtctctgg atcgtcgatg tccacggttg gaagggctgg gggcggccct
      181 tcgagtggct cggacggaac gcgatcgcat cgttcgtcgt ctcgacgctc gcgacgatcc
      241 tcatgctcgc cgtgaagctt tccggtccgg agggaaaacc gcgttcgctc tacgcgacga
      301 tctaccgcgt cgtgttcgac cggttcgcgg acgcgaggct ggggtcgctc ctcttttccc
      361 tgagctatct cgcgatctgg ctggcggtct tcggcgtcct gtatcgcaaa cgggtgttcg
      421 tgaagatctg aaaaggggcg cgacgaatct gccgcgcccc cggctttcag ccgttcctac
      481 gactgggagg gcgtctccat gtggatgacc gcgaagtcgg caccctgcgg gtcgttcagg
      541 attgcgaagc gtcccacgtt cggaatgtcg gtcggcggca tcatcacctt tgcccccatt
      601 ccgctggctt tgctgaccgt gcggtccgaa tcgtccaccg cgaaatagac gagccagtgg
      661 ggcgggacct tccattcgct ggggagttcc atcatcccag ccgcctgcgc gtcgcccttc
      721 ttgaagatgg tgtactcctg gccggtcggc agaggcatcg tctccctcgt ccagccgaag
      781 aggcgggcgt aaaaatcgcc agcctttttc ggatccggcg tgtagagctc gttccagcag
      841 aaggcgccgg gctcgttgac gacctccgcg ccggtatgct ttttcgcctc ccagaccgcg
      901 aacgttgccc cggtcgggtc ctggatgttg gccattcgac cctggtccat gacgtcgaac
      961 ggctcggtca ggatcttgcc accgagctgt cttgatttcg ccgcggcgtc gtccgcgctc
     1021 gcgacccgga tgtaggagtt ccagtgcggc ggcgtgcctt tctgctgtgg cgtgagctca
     1081 tagagcgcgc cggcctcttt gtggtccttc tccagcatcg agtaggtggc tccgtctcct
     1141 gtcgggagct cgttgactcg ccaatcgaag agcgacgtgt agaacttctt cgccgccgga
     1201 ccgtccttcg tcgcgagctc cacccagcag aacttccccg gttcgtgttt gaccctttcg
     1261 cccattgttg aacctccctg agaaaaattg cccaacgccg agactaccgc tgcggcggcg
     1321 ggaggccaac ctcagcaata gagcttcgtg tgaaggtctg gcgcaccgac gtgacctcga
     1381 agtcggacga atcgctacat cgtcagcttg atgaccgcga aacccgcgcc ctgcgggtcg
     1441 gtcaggatcg acatccgtcc gacgttctcg acgtcgaaag gcggaacggt aacgccggca
     1501 gagagcgac
//
LOCUS       DASWAV010000228         2509 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U2665
            k141_212821, whole genome shotgun sequence.
ACCESSION   DASWAV010000228 DASWAV010000000
VERSION     DASWAV010000228.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960316
            Sequence Read Archive: SRR10260007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2509)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2509)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:47:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,929
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2509
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_212821"
                     /isolate="SMAG_U2665"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.36444 N 132.31889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2452
                     /locus_tag="VF958_04595"
     CDS             <1..2452
                     /locus_tag="VF958_04595"
                     /inference="COORDINATES: protein motif:HMM:NF014566.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="CPBP family intramembrane glutamic
                     endopeptidase"
                     /protein_id="HEX9148423.1"
                     /translation="SAEPIYRERFPRQLSLSGMFSRRGLRTKRFFLAVVLGYALTAFF
                     FAYQAVFYVVAARFGAWAPADTPYSDLLNTALPWATVLLIGFLPAVSEEGISRMFSIS
                     FLDRLGAGRVLAVVIPALIWGFGHSAYPNQPFYIRGLEVGLAGIVMGILMLRFGVLPL
                     LAWHFTVDAIYTALLMLRSGNAYYVVSGAVAAGILLLPLAAALVSYAKRGGFATEEGL
                     TNGDEGFVPTPPSAALAPEEVPAVRRVPARALAVATAAAVLLVSSFFLPAHRPDSLAE
                     DKAGRAQAEAIARRFLRANGAAAERFHMVSYTGTGFAEDEELRREKPEENGRIPGFSE
                     AAARYVLARGGEAAFERLAKANLPLAYWVVRFFEPEKKEEWKVLVDANRSRVVAFVNP
                     KEEAATAAAPPGADAARARALDAAAKLGYPVRDYAAIDVGTQARPKRTDTTVVLESAK
                     DAVGESKPRLRAVFHGARLAALLPTIKVPESFLRDYSRRSPADWVLLAARVVAIGGFV
                     GVGVILLLRLVRGGGFRWRRIVGPLLVALVGTAIAIVNSTSSIARAYQTEQPYALFRV
                     VVTLSLAISAIAVIAAAGFGFVLLSGARPGWRAALRRSGSLGDALARAAIGAAGTAGL
                     ARWIAVASSRFPSLFEPDPTLPHSLERWLPGYAGFWSAATATFSLAAVAATIALAART
                     HTARQPLWRALAVAGVLLALAPTGARSVGEFAASWIPEILMAAWLAFVAFGLLKDHAA
                     AWVLFGALYFGGLRAGELLVQPAPPDRAAGWTVIVLVLLAATALVAGRRQRPEAAAAE
                     VLVPEPVVPLPPAAANEP"
BASE COUNT          284 a          912 c          873 g          440 t
ORIGIN      
        1 ctcagccgag ccaatctacc gggagcgttt cccgcgccag ctctcgctct ccgggatgtt
       61 ctcgcgccgc ggcctgcgca cgaagcgctt cttcctcgcc gtcgtcctcg gatacgcgct
      121 gaccgcgttc ttcttcgcgt accaggcggt cttctacgtg gtcgccgcgc ggttcggcgc
      181 atgggcgccc gcggacactc cttacagcga cctgctgaac accgcgctgc cctgggcgac
      241 ggtgctcctc atcggcttcc tgcccgccgt ctccgaagag gggatcagcc ggatgttctc
      301 gatctccttc ctcgaccgcc tcggcgccgg gcgcgttctc gccgtggtca tccccgcgct
      361 catctggggg ttcggccact cggcctatcc gaaccagccc ttctacatcc ggggcctcga
      421 agtcgggctc gccggaatcg tcatggggat cctgatgctc cgcttcgggg tcctgccgct
      481 gctcgcctgg cacttcacgg tggatgcgat ctacacggcg ctgctgatgc ttcgttccgg
      541 gaacgcgtac tacgtcgtct ccggcgcggt tgccgcggga attctcctcc tgcctctcgc
      601 cgcagcgctc gtttcctacg cgaagcgcgg cgggttcgcg accgaggaag ggctgaccaa
      661 cggcgacgag gggttcgttc cgacgcctcc gtcggcggcg ctcgctcccg aggaagttcc
      721 ggcggttcgc cgcgtacccg cgcgcgcgct ggcggtcgcg acggccgcgg ccgtcctcct
      781 cgtgagctcc ttcttcctgc cggcgcaccg cccggactct cttgccgaag acaaggccgg
      841 ccgcgcgcag gcggaggcga tcgcgcgtcg ctttctccgc gcgaacggcg ccgctgccga
      901 gcggtttcac atggtctcct acaccggcac cgggttcgcc gaggacgagg agcttcggag
      961 ggagaagccg gaggagaacg gccggattcc cgggttctcg gaggcggcgg cgcgttacgt
     1021 cctcgcgcgc ggcggggaag cggctttcga gcggctcgcg aaggcgaacc ttccgctcgc
     1081 gtactgggtc gtgcgcttct tcgagccgga aaagaaagag gagtggaaag tcctcgtgga
     1141 cgccaatcgc tctcgcgtcg tggcgttcgt gaacccgaag gaggaggcgg cgaccgccgc
     1201 cgcaccgccg ggagccgacg ccgcgcgggc gcgcgcactc gacgccgcgg cgaaactcgg
     1261 atatccggtg cgggattacg ccgcgatcga cgtcgggacg caggcccgcc cgaagcgcac
     1321 ggacacgacg gtcgtcctgg agtccgcgaa ggacgcggtc ggggagtcga agccgcgcct
     1381 gagggcagtt ttccacggcg cgcggctcgc cgcgctcctc ccgacgatca aggtccccga
     1441 gagcttcctg cgcgactaca gccggcgcag tcccgccgac tgggtgcttc tggcggcgag
     1501 agtcgtggcg atcggtggtt tcgtcggcgt gggcgtgatc ctcctcctgc ggctcgtccg
     1561 cggcggcggc ttccggtggc gccgtatcgt gggccccctc ctcgtcgctc tcgtcggcac
     1621 tgcgatcgcg atcgtgaact cgacttcgag tatcgcccgc gcctaccaga ccgagcagcc
     1681 ctacgcgctg tttcgggtgg tcgtcacgct gtcgctcgcg atcagtgcga tcgccgtgat
     1741 cgctgccgcg ggcttcgggt tcgtgctcct ttcgggcgcg aggcccggct ggagggccgc
     1801 gctccgacgg agcggctctc tcggcgacgc actcgcccgg gccgcgatcg gcgccgccgg
     1861 cacggccggt ctcgcccgct ggatcgcggt cgcatcgtcg cgctttccct cgctcttcga
     1921 gccggacccg acgctgccgc actcgctcga acggtggctg ccgggatacg cgggcttctg
     1981 gtcggcggcg actgccacgt tttcactcgc ggcggtcgcg gcgacgattg cgctcgcagc
     2041 ccgaacgcac acggcgcgcc agccgctgtg gcgcgctctg gccgtcgcgg gagtgctcct
     2101 cgcgctcgcg ccgaccggcg cgcggtcggt cggggagttc gccgcctcct ggatccccga
     2161 gatcctgatg gcggcgtggc tcgcgtttgt ggcgttcggc ctgctcaaag atcacgcggc
     2221 ggcgtgggtg cttttcggcg ctctctactt cggaggcctg agggccgggg agctcctggt
     2281 ccagccagcg ccgccggatc gggccgcggg ctggacggtg atcgtgctcg ttctgctcgc
     2341 cgccacggcg ctcgttgccg gaagacggca gcggccggag gccgcggccg ccgaggttct
     2401 cgtccccgaa ccggtcgttc cccttcctcc cgcggccgcc aacgaaccat gagaccgctg
     2461 cgaacggtct ccctcgcgct ggcggttctc gtggccgccg tcccccggc
//
LOCUS       DASWAV010000229         1833 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U2665
            k141_612052, whole genome shotgun sequence.
ACCESSION   DASWAV010000229 DASWAV010000000
VERSION     DASWAV010000229.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960316
            Sequence Read Archive: SRR10260007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 1833)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1833)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:47:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,929
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1833
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_612052"
                     /isolate="SMAG_U2665"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.36444 N 132.31889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..224)
                     /locus_tag="VF958_04600"
     CDS             complement(<1..224)
                     /locus_tag="VF958_04600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9148424.1"
                     /translation="MKGLPLAIALVVVLGGCATRATSAPDALSPAARAAMTDLAKTTG
                     APVSEITIVSETDTTWSDTCLGCPGKGEMCA"
     gene            complement(221..>1833)
                     /locus_tag="VF958_04605"
     CDS             complement(221..>1833)
                     /locus_tag="VF958_04605"
                     /inference="COORDINATES: protein motif:HMM:NF024634.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HEX9148425.1"
                     /translation="RRAFARPRRLDLTADAWFILFLIALLIVADLVSEGARVALAPSL
                     AREWSPAVLAVARMMQGLPRETLQGIYEAGWWTHLLDILFFGNYLPYSKHFHIITSIP
                     NVFFMKLEPMGRLGTPNLETAEHFGVSKVEDFAWKSVLDGYSCTECGRCRVVCPTALT
                     GKPLDPKVFIGDVRDAVYEATPQILAATSGRGNGGAAGERKELIGGWISEETIWACTT
                     CGYCTWACPVFIIPTVDKITEMRRHLVLERAEFPREMQTAFRGMETNGNPWGISASSR
                     ADWAKDLPVVTMAEAKGSDIDVLFWVGCAGSYEDRAKKVSRALVEILNEAGVSFAILG
                     TEETCTGDSARRMGNEYLFQMLAQQNVETLNGYGVKKIVTNCPHCYNCIRHEYAPFGG
                     NYEVLHATELVSRLIDEGRIVLSEPVPETVSYHDACYLGRYNGIYQPPRRVLRAIPGL
                     EMKELPRTCERGLCCGAGGGRMWMEEKLGTRINQERMREIEGGATEGVGLSCPYCMIM
                     LGNAKEEIGGETAPFDVLELARRSMAAR"
BASE COUNT          287 a          640 c          603 g          303 t
ORIGIN      
        1 gcgcacatct cccctttccc cgggcaaccc aggcaggtgt cggaccaggt cgtgtcggtc
       61 tcggacacga tcgtgatctc ggacaccggc gcccccgttg tcttcgcgag gtccgtcatc
      121 gcggcccgag ccgcgggcga aagcgcgtcc ggggcgctcg tcgcccgcgt cgcgcacccg
      181 ccgagcacga cgacgagggc gatcgcgagc gggagacctt tcatcgcgcg gccatggatc
      241 ggcgcgcgag ctcgaggacg tcgaagggag ccgtctcgcc gccgatctcc tccttcgcgt
      301 tcccgagcat gatcatgcag tacgggcagg agagaccgac cccttcggtc gcgcccccct
      361 ctatctcgcg catgcgctcc tggttgatcc gcgtcccgag cttctcctcc atccacatcc
      421 gaccgccccc ggcgccgcag cagagtccgc gctcgcaggt ccgcggcagc tccttcatct
      481 cgagccccgg gatcgcgcgc aacacgcgcc gcggcggctg gtagatgccg ttgtagcgac
      541 cgaggtaaca cgcatcgtgg tacgacacgg tctcgggcac cggctccgag aggacgatgc
      601 gcccctcgtc gatcagccgc gagacgagct cggtcgcgtg caggacctcg tagttgccgc
      661 cgaagggcgc gtactcgtgt cggatgcagt tgtagcagtg gggacagttg gtgacgatct
      721 tcttcacgcc gtaaccgttc agcgtctcca cgttctgctg cgcgagcatc tggaagaggt
      781 attcgttgcc cattcggcgt gcggagtccc ccgtgcacgt ctcttccgtc ccgaggatcg
      841 cgaaagagac tcccgcctcg ttgaggatct cgaccagcgc gcgcgagact ttcttggccc
      901 ggtcctcgta ggagcccgcg cagcccaccc agaagaggac atcgatgtcg ctccccttcg
      961 cttccgccat cgtgacgacc ggcaggtcct tcgcccagtc ggcgcgcgag gacgcggaga
     1021 tcccccacgg gtttccgttc gtctccatgc cgcggaacgc ggtctgcatc tccctgggga
     1081 actcggcgcg ctcgagcacg aggtgccggc gcatttccgt gatcttgtcg accgtcggga
     1141 tgatgaagac cgggcacgcc cacgtgcagt agccgcaggt cgtgcacgcc cagatcgtct
     1201 cctccgagat ccagccgccg atgagctcct tgcgttctcc cgcggcgccg ccgttgcccc
     1261 ggccggacgt cgcggccagg atctgcgggg tcgcctcgta gaccgcgtcg cggacgtcgc
     1321 cgatgaagac cttcgggtcg agcggcttcc cggtcaacgc cgtcgggcag acgacccggc
     1381 accgaccgca ctccgtgcag ctgtatccgt ccaggaccga cttccaggcg aagtcctcga
     1441 ccttcgagac gccgaagtgc tccgcggtct cgaggttggg cgtgccgagc cggcccatgg
     1501 gttccaactt catgaagaag acgttgggga tggacgtgat gatgtggaag tgcttcgagt
     1561 acggcaggta gttgccgaag aagaggatgt cgagcaggtg cgtccaccag cccgcttcgt
     1621 agattccctg aagggtttcg cggggaaggc cctgcatcat cctcgcgacc gcgagcaccg
     1681 ccggcgacca ctcgcgcgcg agcgacggcg ccagggccac ccgggcgccc tctgagacga
     1741 ggtccgccac gatcaagagc gcgatcagga acaggatgaa ccaggcgtcc gcggtcaggt
     1801 cgaggcggcg gggacgcgcg aacgcgcgcc gga
//
LOCUS       DASWAV010000230         4008 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U2665
            k141_732327, whole genome shotgun sequence.
ACCESSION   DASWAV010000230 DASWAV010000000
VERSION     DASWAV010000230.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960316
            Sequence Read Archive: SRR10260007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 4008)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4008)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:47:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,929
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4008
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_732327"
                     /isolate="SMAG_U2665"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.36444 N 132.31889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            322..633
                     /locus_tag="VF958_04610"
     CDS             322..633
                     /locus_tag="VF958_04610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9148426.1"
                     /translation="MARPATAAARTAAPIPSPTPTAVSMPVVTAPPASGQDRAPGVLY
                     EQYVPTVVVVTPSTTPISTRAVSPRSTRPPRTPTPAVTQKPGVYYEDPEGRPLTPTPQ
                     S"
     gene            704..2359
                     /locus_tag="VF958_04615"
     CDS             704..2359
                     /locus_tag="VF958_04615"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006098276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vanadium-dependent haloperoxidase"
                     /protein_id="HEX9148427.1"
                     /translation="MTSKAPRPDGLDRRSFLGRLGVGAASIAGLAASAGAATSKADPP
                     IPGKDIGALLDTPEARTARAFEIRMRAAELARDRAIVAQTANGEEQLYPNRIANYSKG
                     LPHNAFGEVDPTAYDSFVGALRSGDPARFEALPMGLGRKLTNPLAGMAFDLEGPDGHH
                     LALPPAPAIASGEGGSELGEVYWMALARDVHFSDYGSSPIIADAAADLSRFPDFRGPK
                     AAGGQVTPETIFRGSTAGDLIGPYLSQFMWLGFHMGALTVAQRVWTLPAGTEYLTQYG
                     DWLASQNGFEDYSYPIDSTPRYIRNLRDLGQWVHVDALYQAYHQACLILLDIGAPVAP
                     GVPYRSSATQIGFGTFGGPHILSLVTEVASRALKAIWYQKWFVHRRLRPEAFAGLVHQ
                     TRTGRNYPVSRDVLNSAALDRLYSRNGTYLLPTIAPEGSPTHPSYGAGHATVAGACVT
                     ILKAWFDGSYELPQRVVPDASGTSLLAYAGPALTVENELNKLAANVAIGRNGAGIHYR
                     ADYWESVKLGEKIAVGILEEQRETYREICNFSFRGFEGQTITI"
     gene            complement(2468..3475)
                     /gene="ligD"
                     /locus_tag="VF958_04620"
     CDS             complement(2468..3475)
                     /gene="ligD"
                     /locus_tag="VF958_04620"
                     /EC_number="6.5.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011686781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-homologous end-joining DNA ligase"
                     /protein_id="HEX9148428.1"
                     /translation="MSPRGPATRDFHPARRSATAVRRRKSDPKRLVIPREPDDVVVEV
                     GGRAVRLTNVNKPFWPELGITKGDLLQYYADVSEWLIPHLTDRAMVMKRYPHGAAGEF
                     FFMKRAPSPRPDWIELCSIEHSSGNVIDFPMVQDLPALLWVVNLGCIDLNQWYAKCDD
                     VDRPDYLHFDLDPVPGANFDKVRETAFLVHESLDSLGMPNYPKTTGSKGIHVYVPIVR
                     GPTQKQVWTFAKEFARQMEARKPELVTAEYKVAKRPEGRVLVDYNQNAWGRTLASIYS
                     VRPKPRATVSTPVTWKELERGVALEDFRMDNVPARLQKLGDLWEPLLAKTRRIKLEKF
                     F"
     gene            complement(3472..>4008)
                     /locus_tag="VF958_04625"
     CDS             complement(3472..>4008)
                     /locus_tag="VF958_04625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011985825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9148429.1"
                     /translation="RSPEGAPIGDVFSFLSGLYFRGKLAYSREFASPPEGVPGSLVIT
                     PNEGLRSPDESITTTRLKRFARGDIDEKKRSYRQPLLRDARLIAEAAGEACEVVLLGS
                     VASGKYVDVLVKVFGERLLFPISFVGRGDMSRGGLMLRSARSGVELDYVSVAGAVRHG
                     PRPPKLPPLPRRPPEPQP"
BASE COUNT          640 a         1400 c         1335 g          633 t
ORIGIN      
        1 tccgtggctt ctgccgccgt gcacactgcg ccacctcgcc ggcgcgcggc ccgtctgccc
       61 ggaaggagat cgcttctgcg gcgtcgtcgt gtggcgcatg gacgcaacgc agtatcagcg
      121 ctccctgtaa agacggcgaa gcgagagata tgatccggac acttgaaacg tgtattggtc
      181 ttcgcccttt tcgccggcgc ggcagtcctg atccttccga cttgccgatc gacgcagcat
      241 acggtcgcgc ggatcgtcac ccctgccccc gcgccgacag aggccttcgt tccggctgag
      301 cgagctccga ctgcgccgcc agtggcccgg ccggccaccg ccgcggcccg gacggctgcc
      361 ccgattccga gcccgacccc cacggccgtc tcgatgccgg tcgtgaccgc gccgcccgcc
      421 tccggccagg atcgggcccc gggggtgctc tacgagcagt acgtcccgac tgtcgtcgtt
      481 gtgaccccga gcacgacgcc gatctcgacc cgcgccgtct cgccgcgctc gacacggccc
      541 ccgcggaccc cgacgccggc ggtcacccag aagccaggcg tgtactacga ggacccggaa
      601 ggacggccgc tcaccccgac tccgcagagc tgacctcacc ttctcggttg ccgacgtcgg
      661 tgacctgcgc gagaatcgtt cggcttacct cgggagaaca agaatgacat ccaaggcgcc
      721 tcgaccggac ggtctcgaca gacgatcctt tctcggccgg ctcggcgtcg gcgcggcttc
      781 gatcgccgga ctcgccgctt ccgcaggggc agcgacgagc aaggccgatc cgcccattcc
      841 gggaaaagac atcggggcgc tgctcgacac ccccgaggcc cgcaccgccc gggcgttcga
      901 gatccggatg cgcgcggccg agctcgcccg ggaccgcgcg atcgtcgcgc agacggcgaa
      961 cggcgaggag cagctctacc cgaaccggat cgcgaattac tcgaagggac ttccgcacaa
     1021 cgcattcggg gaggtcgacc ccacggctta cgacagtttt gtcggcgcgc ttcggagcgg
     1081 agacccggcg cgtttcgaag ctctgccgat gggcctcggc cggaagctga cgaatccgct
     1141 cgcggggatg gcattcgacc tcgagggacc cgacgggcat cacctcgcgc tgccgcccgc
     1201 gccggccatc gcgagcggtg aaggcgggtc ggagctcgga gaggtctact ggatggcgct
     1261 cgcacgggac gtccactttt ccgactacgg ctcgagcccg atcatcgcgg acgcagcggc
     1321 ggacctctca cgcttccccg atttccgcgg accgaaggcg gccggaggtc aggtcacgcc
     1381 ggagacgatc ttccggggct cgaccgccgg agatctcatc gggccgtacc tctcgcagtt
     1441 catgtggctc ggcttccaca tgggagctct gacggtcgca cagagggtct ggacccttcc
     1501 ggccggcacg gagtacctga cccaatacgg agactggctc gcgtcgcaga acggattcga
     1561 ggactactcc tatccgatcg attcgacgcc gcgctacatc cgcaacctgc gcgacctcgg
     1621 ccagtgggtc cacgtcgacg cgctctatca ggcgtaccac caggcgtgcc tgatcctgct
     1681 cgacatcgga gcgcccgtcg ctcccggagt gccgtaccgg agctccgcga cacagatcgg
     1741 gttcgggacg ttcggcgggc cgcacattct ctcgctcgtg accgaggtcg cgtcgcgcgc
     1801 gttgaaggcg atctggtacc agaagtggtt cgttcaccgg cggctgcgtc ccgaggcgtt
     1861 cgcggggctc gtccaccaga cgcgcaccgg ccggaactac ccggtcagcc gcgacgtcct
     1921 gaactccgcc gcgctcgacc ggctgtactc gcgcaacggt acgtacctgc tgccgacgat
     1981 cgcgccggaa ggctcaccga cgcacccatc gtacggagcg ggtcacgcga cggtcgccgg
     2041 cgcctgcgtc acgatcctga aggcctggtt cgacggctcg tacgagctgc cgcagcgcgt
     2101 cgtcccggac gcttcgggga cctctctgct cgcctacgcc ggacccgcgc tcacggtcga
     2161 aaacgagctc aacaagctcg cagcgaacgt cgcgatcggc cgcaacggcg ccggcatcca
     2221 ctaccgcgcg gactactggg agtcggtgaa gctcggcgag aagatcgcgg tgggaatcct
     2281 cgaggagcag cgcgagacgt accgggagat ctgcaacttc tcgttccgcg ggttcgaggg
     2341 tcagaccatc acgatctgag tctcgcgagg tccgagaccg cggtctgtgg gcgctggcca
     2401 cagcgggaga ggcgacgtct ccgagcgggc ggaggagctt cgacgtcaag tccgctcacg
     2461 gcgagggtca gaagaacttc tccagcttga tgcgccgcgt cttcgcgagc aacggttccc
     2521 acagatcgcc gagtttctgc aggcgcgcgg ggacgttgtc catgcggaag tcctcgagcg
     2581 cgaccccgcg ctcgagctcc ttccaggtca cgggcgtcga aacggtcgcc ctcggcttcg
     2641 gacgtacgga atagatcgaa gcgagcgttc gtccccacgc gttctggttg tagtcgacga
     2701 ggacccggcc ctccggccgc ttggcgacct tgtactcggc ggtgacgagc tcgggcttgc
     2761 gcgcttccat ctggcgcgcg aactctttcg cgaacgtcca gacctgcttc tgcgtcggcc
     2821 ctcgcacgat cgggacgtag acgtgaatgc ccttcgatcc ggtcgtcttc gggtagttcg
     2881 gcatgccgag cgagtcgagc gactcgtgga cgagaaacgc cgtctcccgc accttgtcga
     2941 agttcgcgcc gggcaccggg tccaggtcga agtggaggta gtcggggcgg tcgacgtcgt
     3001 cgcacttcgc gtaccactgg ttcaaatcga tgcacccgag gttgacgacc cacaggagcg
     3061 ccggcagatc ctgcaccatc ggaaagtcga tgacgttgcc cgaggagtgc tcgatcgaac
     3121 acagctcgat ccagtcgggc cgcggagacg gcgcccgctt catgaagaag aactctcccg
     3181 ccgctccgtg cgggtaccgt ttcatcacca tggcgcggtc ggtcaggtga gggatcagcc
     3241 actccgacac atccgcgtag tactggagga ggtcgccttt cgtgatcccg agctccggcc
     3301 agaagggctt gttgacgttg gtgagccgga ccgcgcgccc tccgacctcg acgacgacgt
     3361 cgtcaggctc gcgggggatg acgagccgtt tcgggtccga cttcctgcgg cgcacggccg
     3421 tcgccgatcg ccgcgcagga tgaaagtcgc gcgtcgcggg gccgcgcggg ctcacggctg
     3481 tggttccggc gggcgtcgcg gcagcggcgg cagcttcggc ggccgcgggc cgtgccgcac
     3541 cgcgccggcg accgaaacgt agtcgagctc gacgcccgag cgcgcgctgc ggagcatcag
     3601 tcctccgcgg ctcatgtcgc cgcgcccgac gaaggagatc gggaagagaa gcctctcccc
     3661 gaagactttg acgaggacgt cgacgtactt gccgctggcc accgagccca ggaggaccac
     3721 ctcgcacgcc tcgcctgccg cctccgcgat caaccgggcg tcgcgcagga gcggctggcg
     3781 gtagctgcgc ttcttctcgt cgatgtcccc ccgcgcgaaa cgcttcaggc gagtggtcgt
     3841 gatcgattcg tccggcgagc gcagcccctc gttcggcgtg atgacgagcg agccgggaac
     3901 tccttccggc ggcgacgcga actcgcgcga gtacgcgagc ttgccccgga agtacaggcc
     3961 ggagagaaag ctgaagacgt cgccgatcgg cgcgccctcg ggagagcg
//
LOCUS       DASWAV010000231         5438 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U2665
            k141_732355, whole genome shotgun sequence.
ACCESSION   DASWAV010000231 DASWAV010000000
VERSION     DASWAV010000231.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960316
            Sequence Read Archive: SRR10260007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 5438)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5438)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:47:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,929
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5438
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_732355"
                     /isolate="SMAG_U2665"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.36444 N 132.31889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..910)
                     /locus_tag="VF958_04630"
     CDS             complement(<1..910)
                     /locus_tag="VF958_04630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9148430.1"
                     /translation="MVKGRISAAGCFGRGLKLSLSKPYLAVSVWLMQLLLGSVIVLPV
                     SNALHSLLDLSSNGSRMVADPDYGWWETVRRVHPDLLGKLPEEIENAVGVEGVKSTAL
                     AEVQGIGATAISLALLGIVLHAFALGGIFGALREPQASLVVFGREGMRRFPAFLAFTL
                     AALAAVLAAYRWLYLATGESWRDRMNNLDTEGQAMALTAGRLLVFLLVVAAIKLVADS
                     VRAIWVARPDLPAVSRLFAGVAGAFSRPGRLLGVLLLYVISGAALYAIWIVLDPSAGG
                     EARFALVPLILTQQVFVFLRSLIKVGY"
     gene            complement(904..2904)
                     /locus_tag="VF958_04635"
     CDS             complement(904..2904)
                     /locus_tag="VF958_04635"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: protein motif:HMM:NF013592.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family metallopeptidase"
                     /protein_id="HEX9148431.1"
                     /translation="MSRAKGRSSLLALAGNALPSILALATSASLAAAPLTRPIASYQI
                     SCRLDAEKKVVEGTELLTWTNTTSKPAPTLQFHLYLNAFRNTLSTFWRESGGEHRGNR
                     LPESWGSIELTRMTLADGSDLLPALTYIAPDDGNPHDRTSAEVQLPRPVNPGETISVS
                     IDFRSRLPRVSTRTGWKGDFFLVAQWFPKIAVLEEKGWNCHQFHASSEFFADFGDYDV
                     SIDAPARYRGKVGATGVKVEERDLPGERVLARFKQESVHDFAWTADPNYLVIDDLFRE
                     PGVADTPIHLFLEPEHRGQAGRYLKAVKTAIAGYGRVLGPYPYSTLTVVDPPWGARGA
                     GGMEYPTLITGGTYRIAPAHVQSPESVTVHEFGHQYFYGLLASNEFEEPWLDEGFNSY
                     MEDRVVAAAYGSNRPVLPVFGWRVPINVPMKLPLDENRRYFPVATQDALASPAWKFQT
                     RTSYGAHVYSKTALALSTLERLIGTAKMNRALRLYADRWRFKHPTTLDWIAAVNDATG
                     GDWRWFFDRTFFSSGAVDYAVDEARSERARTPKGLFETDGKLEEKAPAGITGARGWDS
                     VVTVARRGDEAMPVEIVLRFEGGHTYRSAWDGEARWTKFRVANGPRLIEAVVDPGEKI
                     LLDLDRTNNGRLVTPDRRAASRWTARAVFWMQNLVDFLTLAW"
     gene            2931..4973
                     /gene="ligA"
                     /locus_tag="VF958_04640"
     CDS             2931..4973
                     /gene="ligA"
                     /locus_tag="VF958_04640"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007288193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HEX9148432.1"
                     /translation="MRKAALEADPARAGTVDSTAARRARQLRTEILRHERLYYVEGRP
                     EVTDAEFDRLMSELAELERAHPEVATADSPTRRVGGEPAEGFETVEHALPMLSLENAY
                     SWEEAEAWRARLVRALGEEPPGYVAELKIDGLSISVRYEDGVLVRGATRGDGFRGEDV
                     TDNVRTIRSIPLSIPETSPIEARGEVFYPRSAFLRLNAEREAAGEPLFANARNAAAGT
                     VRLLDSRVTSRRRLAAWFYSIVVAKEVPVTQAETLERLRSLGLPTQPHWRRCATFQEV
                     REFVDEWRTARGQVDFDTDGVVIKVDDRRLQEEAGSTAKSPRWALAYKYPPEEAMTVV
                     REISVQVGRTGVLTPVAHFDPVVLAGTTVKRATLHNYEDLARKDVRVGDTVIVDKGGE
                     VIPRVIRVVSEKRPRGSVPFAMPERCPVCGDPIAREPGEVAARCVNPACPAVVREALR
                     HFCSRRAMKIEGLGEKLVDQLVSAGLLSDVASVYDLHTEDLAELERWGEKSAANLLAE
                     IERSKGNDVSRLLFALGIRHVGEKAAATLARQFGSLDALMSADEDELQSVEEVGPNTA
                     KAILAWFSHPRHRELVEKLRTRGVNFLSGASRPSSNGPLAGRSVVITGTLPGIARDEA
                     ARVLETAGAKVSGSVSRKTDFLLAGESAGSKLEKARSLGVRVVSWKEMAGILEGGE"
     gene            4976..5191
                     /locus_tag="VF958_04645"
     CDS             4976..5191
                     /locus_tag="VF958_04645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9148433.1"
                     /translation="MELPSRVVVYCPILELKNKPARLLAISPHGYYEVRIDIGERNHT
                     ALLPIAGTGLVFQEPNVTGEPSMEIER"
     gene            complement(5195..>5438)
                     /gene="glmM"
                     /locus_tag="VF958_04650"
     CDS             complement(5195..>5438)
                     /gene="glmM"
                     /locus_tag="VF958_04650"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: protein motif:HMM:NF012626.4"
                     /note="catalyzes the conversion of glucosamine-6-phosphate
                     to glucosamine-1-phosphate; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HEX9148434.1"
                     /translation="KAPQELRNVRVSRREPLERTPELSSAIESASATLGPRGRVFVRY
                     SGTEPLLRILVEGADANEVRAIAEKLESAARRALGG"
BASE COUNT          925 a         1793 c         1877 g          843 t
ORIGIN      
        1 agtaaccgac cttgatgagc gagcggagga agacaaagac ctgctgggtg aggatgagag
       61 gcacgagcgc gaaccgggct tcgccccccg cggagggatc cagaacgatc cagatcgcat
      121 aaagcgccgc gccggagatc acgtagagca agagaactcc cagaaggcgg ccgggccgtg
      181 agaatgcgcc cgccactccc gcgaacagac gtgaaaccgc cgggaggtcg gggcgggcga
      241 cccagatcgc ccggaccgag tctgcaacga gcttgatcgc cgcgaccacg aggagaaaga
      301 cgagcagtcg ccccgcggtg agcgccatcg cctggccttc cgtgtcgagg ttgttcatcc
      361 gatcgcgcca cgactctccc gtcgcgagat agagccatcg atatgccgcg agcaccgcgg
      421 cgagcgctgc gagcgtgaac gcaaggaagg ccgggaagcg gcgcatgcct tcgcgaccga
      481 agacgaccag tgacgcctgg ggctcgcgaa gagctccgaa gattccgcca agagcgaacg
      541 cgtgcagcac gatgccgagg agcgcgagcg agatcgcggt cgcgccgatc ccctgcactt
      601 cggcgagcgc cgtcgatttc acgccttcga ctccgaccgc gttctcgatt tcttccggaa
      661 gcttgccgag gagatccggg tgcacgcggc ggaccgtctc ccaccacccg tagtccggat
      721 cggcgaccat ccgcgatccg ttcgaggaga ggtcgagcag cgagtgcaac gcgttcgata
      781 ccggcaggac gatgaccgag ccgagcagga gttgcatcag ccatacggag acggcgaggt
      841 aaggcttcga cagcgagagc ttcagtccgc gaccgaagca accggcagcc gagattcgac
      901 ccttcaccat gcgagcgtca ggaagtcgac caggttctgc atccagaaga ccgcccttgc
      961 cgtccaacgc gatgccgccc ggcgatcggg cgtgaccagc cggccgttgt tcgtgcggtc
     1021 gaggtcgaga aggatcttct cgcccgggtc gaccaccgcc tcgatcaacc ggggcccgtt
     1081 agcgacccgg aacttcgtcc accgcgcctc gccgtcccac gcggatcggt aagtgtgtcc
     1141 gccctcgaat cgcagaacga tctcgactgg catcgcctcg tcgccgcggc gcgcgacggt
     1201 cacgacgctg tcccagcccc gcgcccccgt gattccggcc ggggccttct cctccagctt
     1261 cccgtctgtt tcgaacagtc ccttcggagt cctcgctctt tccgacctgg cttcgtcgac
     1321 ggcgtagtcg actgctcccg aggagaagaa ggtccgatca aagaaccacc gccagtcgcc
     1381 acccgtggcg tcgttgaccg cggcgatcca gtcgagcgtc gtcgggtgct tgaaccgcca
     1441 gcgatccgca tagagccgga gcgcccggtt catcttcgcc gtgccgatca gccgttcgag
     1501 ggtcgacagc gccagggcgg tcttcgagta gacgtgcgcc ccgtagctcg tccgggtctg
     1561 gaacttccac gcgggggagg cgagtgcatc ctgcgtcgcg accggaaaat accgccggtt
     1621 ctcgtccagc ggcagcttca tcgggacatt gatcggcacc ctccagccga agaccgggag
     1681 caccggccgg ttcgatccgt acgcggccgc gacgacgcgg tcctccatgt agctgttgaa
     1741 cccctcatca agccagggct cttcgaactc gttcgacgcg agaagcccgt agaagtactg
     1801 gtggccgaac tcgtgcaccg tgacgctctc ggggctttga acgtgggcgg gggcgattcg
     1861 ataggtcccc ccggtgatga gggtcggata ctccatgcct ccggcacccc gggcacccca
     1921 gggcgggtca accacggtca gggtcgagta cggatagggg ccgaggacgc ggccgtagcc
     1981 ggcgatcgcc gtcttcacgg ccttgaggta tcggcccgcc tgtccgcggt gctccggctc
     2041 gagaaagaga tggatcggcg tgtccgcgac gcccggttcg cggaagagat cgtcaatgac
     2101 gagatagttc gggtctgcgg tccaggcgaa gtcgtgaacg ctctcctgtt tgaagcgggc
     2161 gagcacgcgc tcgcccggca ggtcgcgctc ttccaccttt acgcccgtcg cccccacctt
     2221 tccgcgatat cgggcggggg catcgatcga tacgtcgtag tcgccgaagt cggcaaagaa
     2281 ctcgctcgac gcgtggaact ggtggcagtt ccagcccttc tcttcgagca cggcgatctt
     2341 ggggaaccac tgcgccacga gaaaaaagtc gcccttccag ccggtccggg tcgacacgcg
     2401 cggcaggcgc gaacggaaat cgatggagac cgagatcgtc tcgccgggat tcacggggcg
     2461 cgggagctgg acttcggcgg acgtccggtc gtgcgggttg ccgtcgtccg gcgcgatgta
     2521 ggtcagcgcg ggaagaaggt cgctcccgtc ggcgagcgtc atgcgcgtca actcgatcga
     2581 gccccacgac tcgggcagcc ggttgccccg gtgctccccg ccggactcgc gccagaacgt
     2641 cgagagcgtg ttccggaacg cattcaggta gaggtggaac tggagcgtcg gcgccggttt
     2701 cgacgtcgtg ttcgtccagg tgaggagttc cgttccctcg acgacttttt tttcggcatc
     2761 gaggcgacac gagatctggt acgaagcgat cgggcgggtg agcggtgcgg cagcgagaga
     2821 cgccgaggtc gcgagcgcga gaatcgaggg tagcgcgttt ccggcgagag cgagaagcga
     2881 acttctgccc tttgcccgcg acatcggcga gactgtagca tgtgcgcccg atgagaaaag
     2941 ccgctctcga agcggatccc gcgcgcgcgg ggaccgtgga ctcgacggcg gcccggcgcg
     3001 cgcgacagct ccgaacggag atcctccgcc acgagcggct ctattacgtc gagggccgcc
     3061 ccgaggtcac cgacgccgag ttcgaccggc tgatgtcgga gctcgcggag ctcgagagag
     3121 ctcaccccga ggtcgcgacc gccgactcac cgacgcgccg cgtgggcggc gaacccgccg
     3181 aaggattcga gaccgtcgag cacgcgctcc cgatgctttc gctcgagaac gcttactcat
     3241 gggaggaagc ggaggcgtgg cgggcccggc tcgtccgcgc gctcggcgag gagcctcccg
     3301 gctacgtcgc ggagctcaag atcgacggtc tctcgatctc ggtgcgctac gaggacggcg
     3361 tgctcgtccg gggcgcaacc cgaggcgacg gtttccgcgg cgaggacgtg acggacaacg
     3421 tgcgcacgat ccgctcgatc ccgctctcga ttcccgagac gtcgccgatc gaggcgcggg
     3481 gagaagtctt ctaccctcgt tcggctttcc tccgtctgaa cgcggagcgc gaggcggcgg
     3541 gagagccgct cttcgccaac gcgcggaacg cggccgccgg caccgttcgg ctgctcgact
     3601 cgcgggtgac gtcccggcgc cggctcgccg cctggttcta ctcgatcgtg gtcgcgaagg
     3661 aagtgcccgt cactcaggcg gagacgctcg agcggctgcg atcgctcggc ttgccgaccc
     3721 agccgcactg gcggcggtgc gccaccttcc aggaggttcg cgagttcgtc gacgagtggc
     3781 ggaccgcgcg cggacaagtc gacttcgaca ccgacggcgt cgtgatcaag gtggacgacc
     3841 gccggctcca ggaggaggcc ggctcgaccg cgaaatcgcc gcggtgggcg ctcgcgtaca
     3901 agtacccacc cgaggaggcg atgaccgtcg tgcgcgagat ctccgtccag gtcggacgca
     3961 ccggcgtgct cacgccggtc gcgcatttcg atccggtcgt cctcgccggc acgaccgtga
     4021 agcgggccac cctgcacaac tacgaggacc tcgcgcgaaa agacgttcgg gttggtgaca
     4081 cggtcatcgt cgacaagggg ggcgaagtca tcccccgggt gatccgcgtc gtctccgaga
     4141 agcgaccccg cggaagcgtc ccgttcgcga tgcccgagcg ctgtcccgtc tgcggggatc
     4201 cgatcgcgcg ggagccaggg gaagtcgcgg cgcgctgcgt caacccggcg tgcccggccg
     4261 tcgtgcgcga ggcgctccgg cacttctgtt cgcggcgggc gatgaagatc gaaggcctcg
     4321 gcgagaagct cgtcgaccag ctcgtctcgg cgggacttct ctcggacgtg gcgtcggtct
     4381 acgaccttca caccgaagac ctggcggagc tcgagcgctg gggcgaaaag tccgctgcga
     4441 acctgctcgc ggagatcgag cgaagcaagg ggaacgacgt ctcgcggctc ctgttcgcgc
     4501 tcggcatccg gcacgtcggc gagaaggccg ccgccacgct ggcgcggcag ttcggatcac
     4561 tcgacgccct gatgtcggcc gacgaggacg aactgcagtc ggtcgaggag gtcggaccga
     4621 acaccgccaa agcgatcctc gcgtggttct cgcatccgcg gcaccgcgag ctcgtggaaa
     4681 agctccggac tcggggagtg aacttcctct cgggcgcgag ccgtccgtct tcgaatgggc
     4741 cgctcgcggg acggtccgtc gtcattaccg ggacgctccc gggaatcgcg cgcgacgagg
     4801 cggcgagggt tcttgagacc gcgggggcga aggtgtccgg atccgtgtcg cgcaagaccg
     4861 acttcctcct cgcgggagag tcggccgggt cgaagctcga gaaagcgcgg tcgctcgggg
     4921 tccgggtggt gtcgtggaaa gagatggccg gaatcctgga aggaggggaa tagcggtgga
     4981 gttgccgtcg cgggtcgtcg tctattgccc aattctcgag ctcaagaaca agccggcgcg
     5041 gctgctcgcg atctctccgc acggctacta cgaggtccgg atcgacatcg gcgagcgcaa
     5101 ccacacggcg ctcctcccga tcgccggaac cgggctcgtc ttccaggagc cgaacgtgac
     5161 cggagagccg tcgatggaga tcgaaaggta gagctcaacc gccgagggcg cggcgggcgg
     5221 cgctctcgag cttctcggcg atcgcgcgga cttcgttcgc gtcggctccc tcgacgagga
     5281 tccgcagcag cggctcggtc cccgagtagc gcacgaacac tcgtccacgc gggccaaggg
     5341 tcgcgcttgc gctttcgatc gcgctcgaca gctcgggcgt ccgctcgaga ggctcgcggc
     5401 gggagactcg cacgttgcgc agctcctgcg gagctttt
//
LOCUS       DASWAV010000232         2377 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U2665
            k141_173112, whole genome shotgun sequence.
ACCESSION   DASWAV010000232 DASWAV010000000
VERSION     DASWAV010000232.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960316
            Sequence Read Archive: SRR10260007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2377)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2377)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:47:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,929
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2377
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_173112"
                     /isolate="SMAG_U2665"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.36444 N 132.31889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..866
                     /locus_tag="VF958_04655"
     CDS             <1..866
                     /locus_tag="VF958_04655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9148435.1"
                     /translation="RFDLDFAGERSFREAAGRRPPVRGEIAWAGLPTGSIEDATVGGG
                     CGRFEPAAGLVDFERTPGGGIAFPARVNGAELRFALDADADGPPAISGERAASLSLRA
                     SRDVYGRLIASGATLEVGGFTCRGIHIEVGSAPEGADGVIGGTLFREAVVELDPKSGR
                     LGLHDPARWVSPAGFNRILVDDDGNRPVTTLRRGREVARLRAGSETGTSDLRLAPASA
                     ARLDVKTPGVVSELRWGPLALPPLGVSSETDGAAPDWGDDGRLGLAFLLRFHAYVDMT
                     HRWIYVRPNGR"
     gene            918..1094
                     /locus_tag="VF958_04660"
     CDS             918..1094
                     /locus_tag="VF958_04660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012521287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1328 family protein"
                     /protein_id="HEX9148436.1"
                     /translation="MLKLALIFLVIGLVAALFGFTSVAGASFAIAKFLAFLFLVLFLI
                     FLILGLTAVRKITT"
     gene            complement(1220..1441)
                     /locus_tag="VF958_04665"
     CDS             complement(1220..1441)
                     /locus_tag="VF958_04665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9148437.1"
                     /translation="MNEDNVARFDLESRGGMARRGAPDKEREVTRETVEVECPECDAV
                     LCLDASVFALHPEVLCAGCDATIPLAAAR"
     gene            complement(1438..1758)
                     /locus_tag="VF958_04670"
     CDS             complement(1438..1758)
                     /locus_tag="VF958_04670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9148438.1"
                     /translation="MKLVWSQPSRPTHDRARHILLLSVLCIFLGGTLVGAGGRDPRQD
                     PRPLRAAVASLLSTGGVLFFVGIALAAVRKARKPSSTLSLAHPIDDPSDLAARAPRFG
                     QEER"
     gene            complement(1985..>2377)
                     /locus_tag="VF958_04675"
     CDS             complement(1985..>2377)
                     /locus_tag="VF958_04675"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF015048.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HEX9148439.1"
                     /translation="LGARLEFDRVDRVRAVIEPIEPHHRGGIGTDAVNGVVLAGLFDL
                     AIGTVGWLTRPDTRSATVTLGMTFLRPTRGDRVVVEARLLRAGTNLTFASAEIFDGAG
                     QVTARCDGSVAVALGRYGEFFPGMRGGM"
BASE COUNT          367 a          825 c          824 g          361 t
ORIGIN      
        1 cccgtttcga cctcgatttc gcgggcgagc ggtcctttcg ggaagcggcg ggacggaggc
       61 cgcccgtccg cggcgagatc gcctgggccg gccttcccac gggaagcatc gaggacgcca
      121 ctgtgggcgg tggatgtggc cggttcgagc ccgccgcagg cctcgtcgac ttcgagcgga
      181 cccccggcgg aggaattgcc tttcccgcac gggtcaacgg cgccgagctt cgcttcgcgc
      241 tggacgccga cgccgacggc ccgcccgcga tctccggaga gagagcggcc agcctgtcgc
      301 ttcgcgcctc gcgggacgtc tatgggcgtt tgatcgcctc cggcgcgacg ctcgaggtcg
      361 gaggcttcac ctgtcggggc atccacatcg aagtcggcag cgcgccggaa ggcgccgacg
      421 gagtcatcgg cggcaccctc tttcgcgaag ccgtggtcga gctcgacccg aagtccggca
      481 ggctcggcct tcacgacccc gcacggtggg tctctcccgc cgggttcaac cgcatcctcg
      541 tcgacgacga cggcaaccgc ccagtgacga cgctgcgaag aggaagagag gttgcgcggc
      601 ttcgagccgg atcggagacc gggacctccg acctgcgact cgcgccggcc tccgccgcgc
      661 gcctggacgt gaagacaccc ggcgtcgtat ccgagctgcg ctggggtccg ctggcgcttc
      721 cgccgctcgg ggtgtcgtca gagaccgacg gcgcggcgcc cgactggggg gacgacgggc
      781 ggctcggcct cgcgtttctc ctgcggtttc acgcctacgt cgacatgacg caccgctgga
      841 tctacgtccg gccgaacggc cggtaggccc gaccggcacg cctttcgcaa tctccgcgcc
      901 ggggaggaga ccggaaaatg ctgaaactcg cgttgatctt cctcgtgatc gggctcgtcg
      961 cagcgctctt cggcttcacg tcggtggccg gagcgtcgtt cgcgatcgcg aagttcctcg
     1021 cgttcctgtt cctggtcctg ttcctgatct tcctgatcct gggcctcacg gcggtccgca
     1081 agatcaccac gtaacaaatt ccccggaccg gaacggttgc ggtctcttcc tttgcggggg
     1141 tgcgcagggc cggctgaggc ggtcgaagcg gaaatccccg gcggaacagg cgttccgccg
     1201 cggtttcttt atcgagaaat cagcgggcgg cagccagggg aatcgtcgcg tcgcaccccg
     1261 cgcagaggac ctccgggtgc aacgcgaaga ccgaggcgtc gaggcagagg acggcgtcgc
     1321 actccggaca ctcgacctcg acggtttcgc gggtgacttc ccgctccttg tcgggcgcgc
     1381 cccggcgcgc cattccgccg cgcgattcga gatcgaagcg ggctacgttg tcttcgttca
     1441 ccgttcctcc tgtccaaagc gcggcgcgcg cgccgccagg tcggaggggt catcgatcgg
     1501 atgcgcgaga gagagagtcg aggagggctt ccgggctttg cgcacggcgg cgagagcgat
     1561 cccgacgaaa aagaggactc cccccgtcga aaggaggctc gccaccgccg cccggagagg
     1621 acgcggatcc tgtcgcggat cgcgcccgcc ggccccgacg agggtcccgc cgaggaaaat
     1681 gcacagcacc gacaggagca ggatgtgacg ggcccggtcg tgcgtgggcc gcgaaggctg
     1741 agaccagacg agtttcaagg cgtcctcacg agacaaggga ctggaggggc cgcgcggccg
     1801 cgttgggagc aaggtccacc accattcccc ctccggcacg tccgggagcg acgccgggag
     1861 ctcccgaatg actgacgcga tcattgtaga ggacggcgtc gcccggcgca atctcgtacg
     1921 acgaaagtcg gagtgtccga acgatcgaga tccgttcgct tcgctcggga tgaccgcgac
     1981 cggctcacat tcccccgcgc atccccggga aaaactctcc gtaccggccg agtgcgacag
     2041 ccacgctacc gtcgcaccgg gccgtgacct gacccgctcc gtcgaagatc tccgcggagg
     2101 cgaacgtcag gttcgttccc gctcgaagca ggcgcgcctc gacgacgacc cgatcgccgc
     2161 gcgtcgggcg gaggaacgtc atgccgagcg tgaccgtcgc ggagcgcgtg tcgggccgcg
     2221 tcagccaccc gacggtcccg atcgcgaggt cgaagagtcc ggcgagcacc acgccgttga
     2281 ccgcgtcggt cccgattccg ccgcggtggt gcggctcgat cggctcgatc acggcgcgca
     2341 cgcggtcgac ccggtcgaat tcgagccgcg ctcccag
//
LOCUS       DASWAV010000233        11131 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U2665
            k141_212967, whole genome shotgun sequence.
ACCESSION   DASWAV010000233 DASWAV010000000
VERSION     DASWAV010000233.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960316
            Sequence Read Archive: SRR10260007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 11131)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11131)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:47:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,929
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11131
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_212967"
                     /isolate="SMAG_U2665"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.36444 N 132.31889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1033)
                     /locus_tag="VF958_04680"
     CDS             complement(<1..1033)
                     /locus_tag="VF958_04680"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEX9148440.1"
                     /translation="MPLTVGDLLGPYEILGPLGQGGMGEVYRARDTRLRREVAVKVLR
                     EEIANDPDSLSRFRRETHAVAALNHPNILAIHDAGEEGGLPYAVTELLDGETLADRLA
                     AGPLPARRAVEIAAQVADGLAAAHEKGIFHRDVKPANVFLTRDGRIKILDFGIARIGA
                     VASLADDAGTVTQEASSRQLIGTVPYMSPEQLRGQLADGRSDIFALGAVLYEMLTGRK
                     AFARAAPAETISAVLREDPMAAGEATGLPPVLGQLLSRCLEKEPSDRYQSARDLFLDL
                     RAFAAAPTKTELAAVGARRAGPGRLAVAAAAGLVLFLSGLFAGSRLGRGNRATTRGAT
                     PLMDLILPLD"
     gene            complement(1037..2083)
                     /locus_tag="VF958_04685"
     CDS             complement(1037..2083)
                     /locus_tag="VF958_04685"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEX9148441.1"
                     /translation="MALSNGDQLGPYEIVAPLGKGGMGEVYRARDVRLGREVALKVLP
                     ETAANDTESLARFEREARAVAALNHPNILSIHDTGEHRGVPYAVTELLEGEILSDRLR
                     AGPLAPKRATEIACQIADGLAAAHARGVIHRDIKPENIFLTNDGRAKILDFGIARIGP
                     KPGDITQGIAAARGSSAQFVMGTAGYMSPEQVRGKPIDFRTDIFSLGACFFEMLTGRR
                     AFDRPSAVETMTGVLKDDPAKMPEAEKIPEAIRPFVFRCLEKDPADRYQSARDLLLDL
                     RAYQAEGLRDAAERVGQFRAEPPWKHRKTRVYLRAAGGVLIFILGFFSGSCWQKARVP
                     LTGRSAPIATAPTR"
     gene            2231..2722
                     /locus_tag="VF958_04690"
     CDS             2231..2722
                     /locus_tag="VF958_04690"
                     /inference="COORDINATES: protein motif:HMM:NF012521.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HEX9148442.1"
                     /translation="MDVWLLRHAAAQDRAPSGRDEDRELTPEGFARATAVGRGLAALS
                     PPIAIVLTSPFSRARQTAQACATALGLASPHPFAPLLPGTDPSHAVEGLALGGWPSVL
                     LVGHQPLLGALVGRFVFGDERHEIPLRKAAIVRVSWTPGVAGRLEGFLPPEVLERLGT
                     AEN"
     gene            2770..3414
                     /locus_tag="VF958_04695"
     CDS             2770..3414
                     /locus_tag="VF958_04695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007413886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HEX9148443.1"
                     /translation="MGKLGLGCVALIVVLAVIVGISMAGTYNRLVTLGQEVDKQWGQV
                     ENVYQRRADLVPNLVATVRGAAEFEKSTLEAVTQARASVGRAQVSAPTAPTDPAQMAQ
                     FEAAQAQLGAALQRLLVVVERYPELKANQNFRDLQAQLEGTENRITVERNRYNETAQA
                     YNTARLRFPTNLFASMFGFKEKAYFKAVTPGAERAPQVQFDFRNATPAAPAPAR"
     gene            3461..4276
                     /locus_tag="VF958_04700"
     CDS             3461..4276
                     /locus_tag="VF958_04700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011342034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HEX9148444.1"
                     /translation="MSRNGSRIVVALLFAAAAAASAFAQKSIEEQLPPKPDRYVTDRA
                     GVLPSDRADALNRKLEQFERDTSNQVLVWIDRKVPENFVLEDFTVRAYQKWRVGQAKE
                     DNGAVLFVFVDDRKLRIEVGYGLEGVLPDITAKRIIEDEIVPRFRGGDYPAGIEAGVN
                     AMIAATKGEYKGSGKTVDERSRRGTGFPIQGCASALFFLFIFVILPMLARRNRRLWRT
                     YGGSGWWTGGGGFGGFGGGGWSGGGGGWSGGGGGGGFSGGGGSSGGGGASGSW"
     gene            4290..4721
                     /locus_tag="VF958_04705"
     CDS             4290..4721
                     /locus_tag="VF958_04705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012372840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HEX9148445.1"
                     /translation="MASFFSKLDSDRIVSAIAEAERRSSGEIRVHVTLRKPDDLESRA
                     RRRFERLGMTNTAGRNGVLFYIAPTVRRFQILGDSGIHERCGPDFWKEVAGEMEESFR
                     RGEFTEGVVRGVGKVGDLLTRHFPRSKADKNELPNAIDEET"
     gene            4781..5101
                     /gene="rplU"
                     /locus_tag="VF958_04710"
     CDS             4781..5101
                     /gene="rplU"
                     /locus_tag="VF958_04710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011714897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HEX9148446.1"
                     /translation="MYAILRAGGKQMKVSAGDVVRVEKPSGKKLAKGEELTLSDVLFV
                     SGDDGIRSAKDDLAKVAVKATVIGEVRSRKVLVFKKKIRKQYRRTKGHRQTMVEIRIN
                     SIEG"
     gene            5107..5367
                     /gene="rpmA"
                     /locus_tag="VF958_04715"
     CDS             5107..5367
                     /gene="rpmA"
                     /locus_tag="VF958_04715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011520826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HEX9148447.1"
                     /translation="MAHKKGQGSSRNGRDSNAQRLGVKRFSGQVVSGGSILVRQRGTR
                     FLPGENVGRGKDDTLFAKVAGTVRFRERGRQGRFISIEPTEA"
     gene            5409..6422
                     /gene="obgE"
                     /locus_tag="VF958_04720"
     CDS             5409..6422
                     /gene="obgE"
                     /locus_tag="VF958_04720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011342357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HEX9148448.1"
                     /translation="MFIDTAEIHVKGGDGGNGCVAFRREKFVPKGGPSGGDGGHGGSV
                     WAVADPALNTLYHLRHQNSYRAGRGAHGMGSNRHGKSGESVVIRLPLGSVVRDAESGE
                     PLAELLSAGERVELAKGGNGGWGNQHFATPTRQAPRFAKPGQPGEERQLAIELKLLAD
                     VAIIGLPNAGKSTLISVISAAKPKIADYPFTTLVPHLGVVADEGSRTLVVADIPGLIE
                     GAHLGAGLGIRFLKHVERCRLLCHLVDASGEGDAESAVAVLERELEQFSPEVAARPRV
                     LVASKADAVSDPERLESIRRAAAARGLPFFEVSAVTRKGLDRLVHYFFQAVPPAETAR
                     AAS"
     gene            6433..7032
                     /gene="nadD"
                     /locus_tag="VF958_04725"
     CDS             6433..7032
                     /gene="nadD"
                     /locus_tag="VF958_04725"
                     /inference="COORDINATES: protein motif:HMM:TIGR00482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000309 - nicotinamide-nucleotide
                     adenylyltransferase activity [Evidence IEA];
                     GO_function: GO:0004515 - nicotinate-nucleotide
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate (nicotinamide) nucleotide
                     adenylyltransferase"
                     /protein_id="HEX9148449.1"
                     /translation="MRIGVFGGTFDPVHDGHVKPVSRAAELFGLERVIWVPALLSPHK
                     GTPPTDARHRVAMVALAIAGRPDWSISFDELDRDPPSYTVDTLRALSARFLRDELWLL
                     MGTEALAGFGRWKEPEEIVRLARIAAFHREPFVGDRLQIPEVAGLRNRLEVFDGGSFK
                     ISSTELRYDLGRGGTAAGRVPEPVAEYITKHRLYREVRG"
     gene            7029..7382
                     /gene="rsfS"
                     /locus_tag="VF958_04730"
     CDS             7029..7382
                     /gene="rsfS"
                     /locus_tag="VF958_04730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015563262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HEX9148450.1"
                     /translation="MTADHTSQDKVREGLTAALDRKASAVLVFNLTDLTTMADYFVLT
                     TAINDRQARAIADSIVERLGPALSVEGLQTATWILMDYGDVVFHIFQAEARRFYALER
                     LWGDAANETGRFAAS"
     gene            7379..8353
                     /locus_tag="VF958_04735"
     CDS             7379..8353
                     /locus_tag="VF958_04735"
                     /inference="COORDINATES: protein motif:HMM:NF012385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HEX9148451.1"
                     /translation="MSSEAPERPATRRRRSLKSLFRTRNRVLAREIAMLERALDLPTT
                     ILILGESGTGKDRLARALHDASDRRDRPFVRVEAANLSDDLFESELFGHERGAFTGAV
                     TGKRGLLEVATDGTVYLDEVSSLSLAGQAKFLRVLQEKTFRRLGGVIVHPFRAKLIVS
                     SRRDLATLVREKAFRDDFFYRIDVVSVRLPRLADRPEDVLPLARESLRRSSRAYRRPA
                     RRFSTEAEELLVRHPWPGNVRELVHLVEKAVLTADSTTISAADLPFGALGSPDSLLAT
                     AVEKRWTLKEISDAYIEETLRRAGGNRSLAAKRLGVSRKFLWERAKKK"
     gene            8499..8888
                     /locus_tag="VF958_04740"
     CDS             8499..8888
                     /locus_tag="VF958_04740"
                     /inference="COORDINATES: protein motif:HMM:NF013427.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA family transcriptional regulator"
                     /protein_id="HEX9148452.1"
                     /translation="MATSAARVPKRNKRELEKYRKLLEQKKAELSNELAKARSAEEES
                     NEEATQDIADKAVSSYTREFLYSLTDGERTVLLQIDEALARIDDGTYGFCTNCGASMN
                     EKRLTAVPWTPYCVDCQELSEKGLLEG"
     gene            8992..10356
                     /locus_tag="VF958_04745"
     CDS             8992..10356
                     /locus_tag="VF958_04745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9148453.1"
                     /translation="MPKKEFAEEAVPPFDSAPSVIAIEGKIPFFVEEAASRARERLAG
                     GDVEVLRFGDEAPPGTVAEALLNRSLFSPKRLVEIDISRLLGTDSPGELAESAVQAWE
                     RGSPSARREAFRHVRRLLSAVDMAVEGDPEEAAAGLARKLRKKDLAESLAAILREMPE
                     ERDTSGATLTSTLRLLLERGNDGLVALLTASDPPQGSELVAAVAKAGLLLSVRVSDDR
                     KEVEPALRRLAAARAKEREIRIDPDAVSRLLLRTDARPDVFGAELEKLFDWAGEGGQV
                     RAADVGDQVADEASEDFYQFMDFVGMRDAGEALKRLERMFSGREVRAGKRSFDPEEEG
                     GWPQIVLGMLTSEIRRMLLIRARLDEPGAPAFDARMDFRVYQRRVAPFLDEPIVPFGR
                     SPFGGRAETYPFYKAARGAARFTSKELARSLSRAADVDVKLKNSAPVLETLTAYVGEL
                     IAGH"
     gene            10377..>11131
                     /locus_tag="VF958_04750"
     CDS             10377..>11131
                     /locus_tag="VF958_04750"
                     /EC_number="1.1.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020707984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose 1-dehydrogenase"
                     /protein_id="HEX9148454.1"
                     /translation="MPISIDLSGRTALVTGGSRGIGRAAAEILAAAGARVAINYRTND
                     AAAEAFVRETRAAGGEALALAGDVGNPREAHQLVSDVVAAWGRLDVLVNNAGIWEEDE
                     AGSGDLDVWKRTLDVNVLGAHVVTDAAIPHLERERGSIVFVSSTAGQRGEARHSAYAA
                     SKGALISYTKSLAAELGPRGIRVNCVAPGWVDTEMTARALGDPDVRAEIERAIPLGRV
                     ARPGDVAGAILFLVSDLARHVQGEILNVNGGSVL"
BASE COUNT         1832 a         3686 c         3868 g         1745 t
ORIGIN      
        1 ggtcgagggg gaggataagg tccatgagcg gcgtcgcgcc tcgcgtggtg gcgcgatttc
       61 cccggcccaa ccggctgccg gcgaagagcc cggaaaggaa gagaacgaga cccgccgctg
      121 ccgcgaccgc gagtcgacca ggacccgctc gccgtgctcc cacagctgca agctcggtct
      181 tcgtgggcgc cgcggcgaac gcccgcagat cgagaaacag gtcgcgggcg gactgatatc
      241 ggtccgaggg ttccttttcg agacagcggg agaggagctg gccgaggacg ggcggcagcc
      301 cggtagcttc gccggccgcc atcggatcct cccgcagaac cgcactgatc gtctccgcgg
      361 gcgccgcgcg ggcgaaagcc tttctgccgg tcaacatttc gtagaggacg gcgccgagag
      421 caaagatgtc cgagcggccg tctgccagct gcccgcggag ctgttcgggc gacatgtatg
      481 gaacggtgcc gatcagctgc cgggagctcg cctcctgagt gaccgttccg gcatcgtctg
      541 cgagtgaggc gacagccccg atgcgcgcga tgccgaagtc gaggatcttg attcgaccgt
      601 ctctcgtcag gaagacgttc gcgggcttca cgtcgcggtg gaagatccct ttttcgtgcg
      661 cggcggcgag cccgtcggca acctgggccg cgatttcgac cgcgcgcctc gcgggaagag
      721 gtcccgctgc gagccggtcc gcgagggtct cgccgtcgag cagctccgtg accgcatacg
      781 ggagaccacc ctcctcaccc gcatcgtgga tcgcgaggat gttcggatgg ttcagcgccg
      841 cgacggcatg cgtctcgcgg cggaagcgcg agagactgtc ggggtcgttc gcgatctctt
      901 cccggaggac tttcacggcc acttcgcggc gaagccgcgt gtcgcgcgcc cggtagacct
      961 cgcccatgcc gccctgcccg agcggtccca gaatctcgta ggggccgagg aggtccccga
     1021 ccgtgagcgg cacgctctac cgcgttggcg cggtcgcgat gggcgcggag cgaccggtca
     1081 agggcacgcg cgctttctgc cagcacgagc cggagaaaaa cccgagaatg aaaatcagca
     1141 cccctcccgc cgcgcgaagg tagacgcgcg tcttgcggtg cttccacggc ggctcggcgc
     1201 gaaactggcc gacgcgctcg gcggcgtcgc gcaagccttc cgcctggtac gcccgcaggt
     1261 cgagcagaag gtcgcgcgcc gactggtaac ggtccgccgg gtctttctcc agacagcgga
     1321 agacgaacgg gcggatcgcc tcggggatct tctccgcctc cggcatcttc gcgggatcgt
     1381 ctttcaggac gccggtcatc gtctcgacgg cgctcggccg gtcgaaggcg cgtcgcccgg
     1441 tcagcatctc gaagaagcac gcgccgagag agaagatgtc cgtgcgaaag tcgatcggct
     1501 tgccccgaac ctgctcgggc gacatgtagc ccgcggttcc catgacgaac tgcgcgctcg
     1561 agccgcgcgc ggcggcgatg ccctgggtga tgtctcccgg cttcgggccg atgcgggcga
     1621 tcccgaagtc gagaatcttc gcccgaccgt cgttcgtcag gaagatgttc tccggcttga
     1681 tgtcgcgatg gatgacgccg cgcgcgtgcg cagccgcgag cccgtcggca atctggcagg
     1741 cgatctccgt cgctcgcttc ggagcgagcg gtcccgcgcg gagccggtcg gaaaggatct
     1801 ctccctcgag gagctccgtg acggcgtagg ggacgccgcg gtgttccccc gtgtcgtgga
     1861 tcgagaggat gttgggatga ttcagcgccg cgacggcacg cgcttcgcgt tcgaaccggg
     1921 cgaggctctc cgtgtcgttg gccgccgtct cggggagcac cttgagcgcg acctcgcgcc
     1981 cgagccgaac gtcgcgggcg cgatacacct cccccattcc gcccttgccg agaggcgcaa
     2041 cgatctcgta cggtccgagc tgatccccgt tggaaagcgc caagtcgttg aaaggatacg
     2101 ccaacagggt agggggtcag gtggggggtc aggtctccac ttgccacttg cttcgcaaga
     2161 tggcaagacc actcaagcga atgtgtccgt acggcacttc gtgaagacct gaccccgcta
     2221 gactctcagg atggacgtct ggctgctccg acacgcggcg gcgcaggacc gcgcaccttc
     2281 gggccgcgac gaggatcgcg agctcacacc cgaggggttc gcacgggcga cggcggtcgg
     2341 acgggggctg gccgcgctct cgccgccgat cgcgatcgtc ctcacatcgc ccttttcccg
     2401 cgcacgtcag acggcccagg cctgcgccac cgcgctcggc ctcgcatctc cgcacccatt
     2461 cgcgcctctg ctgccgggta cggatccgtc gcacgcggtc gaagggctcg cgctcggagg
     2521 ctggccgtcg gtcctcctcg tcgggcacca gccgcttctg ggcgccctgg tcggccggtt
     2581 cgtcttcggc gatgagcggc acgagatccc gttgcggaag gcggcgatcg ttcgtgtgag
     2641 ctggaccccg ggcgtcgccg gcagactcga aggttttctt ccgccggaag tcctcgagag
     2701 gctcggaacc gctgaaaact gacggcgcct gacgctacaa tcgcgcccgc tcgaaaagga
     2761 ggaggcacca tgggaaagct cggtctgggg tgtgtggctc tgatcgtcgt gctcgcggtc
     2821 atcgtcggca tctcgatggc gggcacgtac aaccggctgg tcacgctcgg ccaggaggtc
     2881 gacaagcagt ggggacaggt cgagaacgtg taccaacgac gcgccgacct cgttcccaac
     2941 ctcgtcgcga ctgtgcgggg cgcggccgag ttcgagaaga gcaccctcga agccgttacg
     3001 caggcccgcg cttcggtcgg gcgcgcccag gtgagcgctc cgacggctcc caccgatccg
     3061 gcgcagatgg cccagttcga agcggcgcag gcccagctcg gcgcggcgct gcagcggctg
     3121 ctcgtcgtgg tcgagcggta tccggagttg aaggcgaacc agaacttccg ggacctccag
     3181 gctcagctcg aagggacgga aaaccgcatc acggtggaac gcaaccgata caacgagacg
     3241 gcgcaggcct acaacaccgc gcggctccgg ttcccgacaa acctcttcgc ctcgatgttc
     3301 ggcttcaagg agaaggcgta cttcaaggcg gtcaccccgg gggcggagcg cgcgccgcag
     3361 gtgcagttcg atttccgcaa cgcgacgccc gcagcgccgg cgccggcgag gtaacgacgt
     3421 agccgggaaa cggaaaacgg aaaccgggaa aacggacgcg atgagccgga acggctcacg
     3481 aatcgtcgtc gctcttctct tcgcggccgc agcggccgcg tcggcgttcg cgcagaagtc
     3541 gatcgaggaa caacttccgc cgaagccgga ccgatacgtc acggaccgcg cgggcgttct
     3601 tccatcggac cgggcggacg cgctcaaccg caagctcgag cagttcgaac gcgacacgtc
     3661 gaatcaggtc ctcgtgtgga tcgaccgcaa ggtccccgag aacttcgtcc tcgaggactt
     3721 caccgtccgc gcgtaccaga agtggagggt cggacaggcg aaggaggaca acggcgcggt
     3781 cctcttcgta ttcgtcgatg accgcaagct gcggatcgag gtgggctacg gtctcgaggg
     3841 cgtcctgccg gacatcacgg cgaagcgaat catcgaagac gagatcgtgc cgcgattccg
     3901 cggcggtgac tacccggcag ggatcgaggc gggagtcaac gcgatgatcg cggcgacgaa
     3961 aggcgagtac aaggggagcg gtaagacggt ggacgaaaga tcgcggaggg gcacgggctt
     4021 cccgatccag gggtgcgcca gcgcgctctt cttcctcttc atcttcgtga tcctcccgat
     4081 gctcgcgcgc cgcaatcggc gcctctggcg gacctacggc gggagcggct ggtggaccgg
     4141 cggcggcggg ttcgggggct tcggcggcgg agggtggtcg ggaggcgggg gaggctggtc
     4201 cggaggggga gggggcggcg gcttctcggg cggcggcggg tcgtccggcg gcggcggcgc
     4261 ctccggcagc tggtgaagcg gagcaagtga tggcgtcctt tttctcgaag ctcgattccg
     4321 accgaatcgt ctcggcaatc gcggaggccg agcgccggag ctcgggagag atccgcgtcc
     4381 acgtcacgct ccgaaagccg gatgatctcg agtcccgggc gaggcgccgg ttcgaacgac
     4441 tcgggatgac gaacacggcc gggcgaaacg gggtcctctt ctacatcgca cccacggtcc
     4501 gccgtttcca gatcctcggc gattcgggga ttcacgaacg ctgcggtccc gacttctgga
     4561 aggaggtcgc gggtgagatg gaggagagct tccggcgcgg ggagttcacg gagggcgtcg
     4621 tccggggcgt cggaaaggtc ggcgacctcc tcacccggca cttcccgcgc tcgaaagcgg
     4681 acaagaacga gctgccgaac gcgatcgacg aggaaaccta gccgcaactt gactttcggg
     4741 ccccaacccg tatgattccc cgttcctttc gggaggctcc atgtacgcga ttctccgggc
     4801 cggcggcaag cagatgaagg tttcggcggg cgacgtcgtc cgcgtcgaga agccgtccgg
     4861 gaagaagctc gcgaagggcg aagagctcac gctctccgac gtccttttcg tctcggggga
     4921 cgacgggatc cgctccgcga aggacgacct cgcgaaggtc gcggtcaagg cgacggtgat
     4981 cggcgaggtc cgcagccgca aggttctcgt gttcaagaag aagattcgca agcagtatcg
     5041 ccggacgaaa gggcatcgcc agacgatggt cgagatccgg atcaactcga tcgaagggta
     5101 gaagccatgg cacacaagaa gggacagggc tcctcgcgca acgggcgcga ctcgaacgcc
     5161 cagaggctcg gcgtcaagcg cttctcgggc caggtcgtct ctggcggctc gatcctcgtg
     5221 cgccagcggg ggacgcgctt cctgccgggc gagaacgtcg ggcgcggcaa ggacgacacg
     5281 ctcttcgcga aggtcgccgg caccgtccgg ttccgcgagc gcgggcggca gggcagattc
     5341 atttcgatcg agcccaccga agcgtagcta tcagctcccg gttctcgact gagaactgat
     5401 cggcttcctt gttcatcgac accgcggaaa ttcacgtcaa ggggggcgac ggaggcaacg
     5461 gctgtgtcgc ctttcggcgg gagaagttcg tgcccaaagg aggtccttcc ggcggcgacg
     5521 gcggccacgg aggatcggtc tgggcggtcg ccgaccccgc gctcaacacg ctctaccacc
     5581 tccgtcatca gaactcgtac cgcgcgggcc gcggcgcgca cggaatgggc tccaaccggc
     5641 acggcaagtc gggggagagc gtcgtcatcc gcctgccgct cggttccgtc gtgcgcgacg
     5701 ccgagtcggg cgagccgctc gccgagctcc tctcggcggg agagcgcgtc gagctcgcga
     5761 agggcggcaa cggaggctgg gggaaccagc acttcgcgac cccgacccga caggcgccgc
     5821 ggttcgcgaa gccgggtcag ccgggggagg agcggcagct cgcgatcgaa ctgaaactcc
     5881 tcgccgacgt cgcgatcatc ggcttgccca acgccgggaa gtcgaccctg atctccgtca
     5941 tctccgcggc caagccgaag atcgccgact atccctttac gacgctcgtc ccgcacctcg
     6001 gagtggtcgc tgacgagggg agccgcaccc tggtcgttgc cgacatcccc gggttgatcg
     6061 agggtgccca cctcggcgcc ggcctcggga tccgattcct gaagcacgtc gagcgctgcc
     6121 ggttgctgtg tcacctggtc gacgcttcgg gcgaaggaga cgccgagagc gcggtcgccg
     6181 tgctcgagcg cgagctcgag cagttctcgc cggaggtcgc cgcccggccg cgggtcctcg
     6241 tcgcctccaa ggccgacgcg gtttccgatc cggagcggct cgagtcgatt cgccgggccg
     6301 cggccgcacg cgggctgccg ttcttcgagg tctccgccgt cacccggaag ggcctcgacc
     6361 gcctcgtcca ctatttcttc caggccgttc cgcccgcgga aactgcgcgc gccgcgagct
     6421 aggagcgatt ccgtgcgcat cggcgtcttc ggcggaacct tcgacccggt ccacgacggc
     6481 cacgtcaagc cggtctcccg cgcggcggaa ttgttcggtc tcgagcgagt gatctgggtg
     6541 cccgcgcttc tctcgcccca caagggcact cccccgaccg acgcgcggca tcgagtcgcg
     6601 atggtcgcgc tcgcgattgc cggccgcccc gactggtcga tctccttcga cgagctcgac
     6661 cgcgatccgc cctcctatac cgtcgatacc ctgcgcgccc tctcggcccg cttcttgcga
     6721 gacgaattgt ggctgttgat gggcaccgaa gcgctggccg gattcggaag atggaaggag
     6781 ccggaggaga tcgtccggct cgcccggata gccgccttcc accgggaacc gttcgtcggg
     6841 gaccggttgc agatacccga agtggccgga cttcgaaatc gcctggaggt tttcgacggc
     6901 ggctcgttta aaatctcctc gacggagctg agatacgacc tcggccgggg aggaaccgcg
     6961 gcggggcgtg ttccggaacc ggtcgcggag tacatcacga agcatcgtct ctatcgcgag
     7021 gttcggggtt gacggcggac cacacctcgc aggacaaagt tcgggagggt ctgactgccg
     7081 cgctcgaccg gaaagcgtcg gccgtcctcg ttttcaacct gacggacttg actacgatgg
     7141 cggactactt cgttctgacg accgccatca acgaccgcca ggcgcgtgcg atcgccgact
     7201 ccatcgtcga gaggctcggc ccggcgctct cggtcgaagg tctccagacg gcgacttgga
     7261 tcctcatgga ctacggcgac gtcgtcttcc acatctttca ggcagaggcg cgtcgcttct
     7321 acgccctcga gagactctgg ggcgacgcgg cgaacgagac cggccgcttc gcggcatcct
     7381 gagttctgaa gcgcccgagc gccccgccac caggcggcgc cgctcgctca agtccctttt
     7441 ccgcacacgc aaccgcgttc tcgcccgcga gatcgccatg ctcgagcgcg cgctcgacct
     7501 cccgacgacg atcctcattc tcggggagag cgggacgggg aaggaccggc tcgcccgcgc
     7561 gttgcacgac gcatcggacc ggcgcgatcg cccgttcgtg cgggtcgaag ccgcgaacct
     7621 ctccgacgat cttttcgaaa gcgagctgtt cggccacgag cgcggcgcgt tcacgggcgc
     7681 cgtgacgggc aagcgcggtc tcctcgaggt cgcgaccgac gggacggtgt acctggacga
     7741 ggtctcgtcg ctctcgctcg cgggtcaggc gaagttcctc cgggtgctcc aggagaaaac
     7801 atttcggcgg ctcggcggcg tcatcgtcca cccgttccga gcgaagctca tcgtctcatc
     7861 ccggcgcgac ctcgcgacgc tcgtgcgcga gaaggcgttt cgcgacgact tcttctaccg
     7921 gatcgacgtc gtctccgtcc ggttgccgcg gctcgccgac cgcccggagg acgtgctccc
     7981 gctggcacgc gagagcctgc ggcgatccag ccgggcgtac aggcggccgg cccggcgatt
     8041 ctcgaccgag gccgaggagc tcctcgtgag gcatccgtgg ccggggaacg tccgggaact
     8101 ggtccatctc gtcgaaaagg ccgtgctcac ggccgattcg accacgatct cggcggccga
     8161 ccttccattc ggcgcactcg gttctcccga ttctcttctc gccacggccg tcgaaaaacg
     8221 gtggacgttg aaggaaatct ccgacgcgta catcgaagag acgctgcggc gggccggcgg
     8281 caatcgctca ctcgccgcga agcgcctcgg agtctctcga aagttcctct gggaacgggc
     8341 aaagaagaaa taaccggccg cggcaaggag tttgctctgt accggtcgcc ggaccggcaa
     8401 ccgtgatctg gatcatccgg gaaatcgagt atcatcgcgg gctcgaaaaa agcggccccg
     8461 ggggcccgat acggggacgc agagaaggga gacatggaat ggcgacgagc gctgctcgcg
     8521 tcccgaagcg aaacaagcgg gagctggaga agtaccggaa gctgctcgag cagaagaagg
     8581 ccgagctgtc gaacgagctc gccaaggcga ggagcgccga ggaggagtcg aacgaggagg
     8641 ccacccagga catcgcggac aaggccgttt cgtcctacac gcgcgagttc ctctactccc
     8701 tgacggacgg ggagcgcacc gtgctcctcc agatcgacga ggctctcgcg cgaatcgacg
     8761 acgggaccta cggcttctgc accaactgcg gcgcctccat gaacgagaag cgcctgaccg
     8821 ccgtgccgtg gacgccctac tgcgtcgact gccaggagct ctccgaaaag gggctgctcg
     8881 agggctgacc cgaaggatca ccgaatcccg agggccgcgt ctgaacgggc gcggctcttg
     8941 ctttttggag cggagaatcg cccggttctc cgcgaagtaa gcttcggccg aatgccgaag
     9001 aaagagttcg ccgaggaagc agtcccgccg ttcgactctg cgccttcggt catcgcgatc
     9061 gagggaaaga ttcctttctt cgtcgaggag gcggcgtccc gggcgcgcga gcgactggcg
     9121 ggcggggacg tcgaagtcct gcggttcggt gacgaggccc cgccaggcac ggtcgccgaa
     9181 gccctgctca atcggtcgct cttttcgccg aagcgcctcg tcgagatcga catttcccgc
     9241 ctcctcggca cggactcccc gggcgagctc gccgagagcg cggtccaggc gtgggagcgg
     9301 ggctcgcctt ccgcgcgccg cgaggccttt cgacatgtgc gccggctgct gtcggccgtc
     9361 gacatggccg tagaaggcga cccggaagaa gcggcggcgg gcctggcgcg gaagctccga
     9421 aagaaggacc tcgccgagtc gctcgcggca attctgcggg agatgccgga ggagagggac
     9481 acctcgggag ccactctcac gtccacgctt cggctccttc tcgaacgggg caacgacggc
     9541 ctcgtcgccc tgctgaccgc gtcggatccc ccccaggggt cggagctcgt ggcggccgtg
     9601 gcgaaggccg gtctccttct ttcggtccgc gtctccgacg accgcaaaga ggtcgagccg
     9661 gcgctccggc gcctggcggc ggcgcgtgcg aaggagcggg agatccggat cgaccccgat
     9721 gcggtctccc ggctgctgtt gcgaaccgac gcccggccgg acgtgttcgg ggcggagctc
     9781 gagaaactct tcgactgggc gggcgaaggt ggacaggtcc gcgcggcgga cgtcggcgat
     9841 caggtcgcgg acgaggcttc ggaggacttc taccagttca tggatttcgt cgggatgcgg
     9901 gacgcgggag aggcgctgaa acggctggaa cgaatgttct cgggccgtga ggtccgtgcg
     9961 gggaaacgct cattcgatcc ggaggaggaa ggcggctggc cgcagatcgt cctcggaatg
    10021 ctgacgagcg aaatccgccg catgctcctg atccgcgcgc ggctcgacga gccgggcgcg
    10081 ccggcgttcg atgcgcgtat ggactttcgc gtgtaccagc gccgggtcgc gccgttctta
    10141 gacgagccga tcgtcccgtt cgggagatcg ccattcggcg gccgcgcgga aacgtaccct
    10201 ttctacaaag cggcgcgcgg cgccgcgcga ttcacatcga aggagctcgc ccgctcgctt
    10261 tcgagagccg ccgacgtgga cgtgaaactg aagaactcgg cgccggtgct ggagacgttg
    10321 accgcgtacg tcggcgagct gatcgcgggc cattagcctc ataaaatagc gaccccttgc
    10381 ccatctcgat cgatctctcc ggcagaaccg cgctcgtcac gggcggatcg cgcggcatcg
    10441 gccgcgccgc ggcggagatc ctcgcggccg ccggcgcccg cgtcgcgatc aactaccgga
    10501 cgaacgatgc cgccgccgaa gcgttcgtcc gcgagacgcg cgccgccggc ggagaggcgc
    10561 tcgcgctcgc gggcgatgtc gggaaccccc gcgaggcgca tcagctcgtg tccgacgtcg
    10621 tcgccgcgtg gggacgcctc gacgtcctcg tgaacaacgc cggtatctgg gaggaggacg
    10681 aagccggctc cggtgacctg gatgtctgga agcggacgct cgacgtcaac gtcctcggtg
    10741 cccacgtcgt gaccgacgcc gcgattccac acctggagcg cgagaggggc tcgatcgttt
    10801 tcgtctcctc gaccgcgggg cagcgcggcg aggcgcggca ttccgcctac gccgcctcga
    10861 agggggcgct catctcctac acgaagtcgc ttgccgccga gctggggccc cgggggatcc
    10921 gcgtgaactg cgtcgctccg ggttgggtcg atacggagat gaccgcgcgg gctctcggcg
    10981 accccgatgt ccgtgcggag atcgagcgcg cgatcccgct cggccgcgtc gccaggcccg
    11041 gcgacgtcgc cggtgcgatt ctcttcctcg tttccgacct cgcccgccac gtgcagggcg
    11101 agatcctgaa cgtcaacggc ggcagcgtgc t
//
LOCUS       DASWAV010000234         3537 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U2665
            k141_213105, whole genome shotgun sequence.
ACCESSION   DASWAV010000234 DASWAV010000000
VERSION     DASWAV010000234.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960316
            Sequence Read Archive: SRR10260007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 3537)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3537)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:47:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,929
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3537
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_213105"
                     /isolate="SMAG_U2665"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.36444 N 132.31889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..767)
                     /locus_tag="VF958_04755"
     CDS             complement(<1..767)
                     /locus_tag="VF958_04755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020717798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000902 - cell morphogenesis [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HEX9148455.1"
                     /translation="MAWRSVFSLFSNDLAVDLGTANTLVFARGKGIIVREPSIVAVNN
                     MTNRVEAVGTEAKEMLGRTPGNIVAIRPMKDGVIADFEVTEKMLDHFIKKAHGRSLFV
                     RPRIVISVPSGITQVEKRAVQDSALRAGASEVFIIEQAMAAAIGAGLPITEPAGNMIV
                     DIGGGTTDVAVISLAGIVYSRSVRIASNEMDEAIIQYVKRKYNLLIGERTAEQIKIEI
                     GSAYPLDEPLTMEVKGRDLVEGVPKTLMLTDEEVREAL"
     gene            complement(884..2320)
                     /locus_tag="VF958_04760"
     CDS             complement(884..2320)
                     /locus_tag="VF958_04760"
                     /inference="COORDINATES: protein motif:HMM:NF016648.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FecR domain-containing protein"
                     /protein_id="HEX9148456.1"
                     /translation="MRRRNLGRLVGADLDWFVIPVRKVTIWGLLIVLVAAGSILGYRA
                     YVRGKPTAADRARAEIETASTLVTRATRSAGTVRPGSNVARALGLLQDARTAMGDKHY
                     DEAVRLAVESQSYSRRVLGGWDENGDASFIFVEGDVALQRAGRSNFENARQRQPLFDG
                     DFIKTGRNGSAEIMFSDGTLFTIRPGSLFEVRRPSSSDASGSEVKMVSGAVNVYTSAS
                     PSTIATDDATAAIERDSRVSVDVAAGEKTEVTTYRGKTTVSTGLHTVVLEGRQSVVAA
                     ARTGVLSPRVVLPESPRPLGPDDNRTFDLVTGGDVELKWSRVPEAVRYRIQISRSRLF
                     VPDANEVDLDNRTGLSAEIKVSREGAYFWRLAAIDRAGVSSDWSSVRRFRMSSEPIQA
                     GSGDVTPPELSVLPPQQMGNLFLIFGKTEKGAMVTVNAEPADVSSDGSFKKTVTIERD
                     GYAILVIKAVDAAGNETIRRMRVFVETL"
     gene            2487..>3537
                     /locus_tag="VF958_04765"
     CDS             2487..>3537
                     /locus_tag="VF958_04765"
                     /inference="COORDINATES: protein motif:HMM:NF025010.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SurA N-terminal domain-containing protein"
                     /protein_id="HEX9148457.1"
                     /translation="MRASFHHLKWTLFAVIIVFVLGFVFFSGSNWGRDSSSQVVARIG
                     AEEISAVEFDRIYQNQVQRYREMYKGSFSPELARALDLPRQVLDGMIERKLRIEAAKR
                     MNLAVTDEELARAIAVLPFFQENGLFIGADKYAERLRMAGMAPEAFEEGMREDLLVDK
                     HTALVKASVAVLDADIRREFSAKNDKAKIEYIVVPASRLESALEPSEADLKTYYEKNK
                     ERYRAPEQRRANYLLVDRLKVRAKVPVTDAEIRAEYESKKASFSVPEQVSVSHILVAV
                     DSKGGPEADARAKAKAEALAARAKAGEDFAKLAKENTDDPTGKENGGQLPPFGRGQMV
                     PEFEQAAFDMKPGEIR"
BASE COUNT          637 a         1128 c         1145 g          627 t
ORIGIN      
        1 agcgcttcgc ggacctcttc gtcggtcaac atcagcgtct tcggaacgcc ctccacgagg
       61 tcgcgtccct tgacttccat cgtgagtggc tcatcgagcg ggtaggccga gccgatctcg
      121 atctttatct gctcggcggt gcgctcaccg atcaggaggt tgtacttgcg cttcacgtac
      181 tggatgatcg cctcgtccat ttcgttcgaa gcgatccgca cggagcggct gtagacgatc
      241 cccgcgagcg agatgaccgc gacgtcggtc gttccgccac cgatgtccac gatcatgttt
      301 ccggctggct cggtgatggg aagccccgcc ccgatcgccg ccgccatcgc ctgttcgatg
      361 atgaacacct ccgaggcgcc ggcccgcaac gccgagtcct ggaccgcgcg cttttcgacc
      421 tgcgtgattc cggacggcac cgagatgacg attcggggac ggacgaagag ggatcggccg
      481 tgcgccttct tgatgaagtg atcgagcatt ttctcggtca cctcgaagtc ggcgatgacg
      541 ccgtctttca tcggccggat ggcgacgatg tttcccggcg tgcgcccgag catctccttc
      601 gcctccgtgc cgaccgcctc gacgcgattg gtcatgttgt tgacggcgac gatggaaggt
      661 tctcggacga tgatcccctt gccgcgggcg aagacgagcg tgttcgcggt gccgagatcg
      721 acggcgagat cattcgaaaa aagggagaaa acagatcgcc aggccaaagg agcctcctag
      781 gggccgtaag tcggaagtcg gaagtcggaa gtcggaatga agaagatagc caagttctct
      841 cgaattgtca ttgtcgctcg attcccgatt cccgattacc gtctcagagg gtttccacga
      901 acacccgcat ccgccggatg gtttcgttgc cggccgcgtc gacggccttg atgacgagaa
      961 tcgcgtagcc gtcacgctcg atcgtgaccg tcttcttgaa gctgccgtcc gaggagacgt
     1021 cggccggctc ggcattcacg gtcaccatgg ctcccttctc ggtcttgccg aagatgagaa
     1081 acaggttccc catctgctgg gggggcagga ccgagagttc cggaggcgtg acgtcgccgc
     1141 tgcccgcctg gatcggctcc gacgacatgc ggaagcgccg aacagagctc caatcggacg
     1201 aaactcccgc gcggtcgatc gccgcgagac gccagaagta tgcgccttcc cgcgagacct
     1261 tgatctccgc cgagagaccc gtccggttgt cgaggtcgac ctcgttcgcg tcgggcacga
     1321 agagccggga tcgcgagatc tggatccggt agcgcaccgc ctccggtacg cgcgaccact
     1381 tgagctccac gtcgccgccg gtcaccaggt cgaatgtccg gttgtcgtcg ggtcccagag
     1441 gccggggaga ctcgggcaga acgacgcgcg gggagaggac tccggtccgc gccgcggcga
     1501 ccacgctctg gcgcccttcg agcaccaccg tgtgcaggcc ggtcgaaacg gtggtctttc
     1561 cgcgatacgt cgtcacttcg gttttctccc cggcagcgac gtcgaccgac acgcgagagt
     1621 cccgctcgat cgcggcggtc gcatcgtcgg tcgcgatcgt ggaaggcgac gcggaggtgt
     1681 acacgttgac tgcgccggag accatcttca cttcactgcc gctcgcgtcc gacgacgacg
     1741 gccttcgcac ttcgaagagc gaacccggtc gaatcgtgaa cagtgttccg tcactgaaca
     1801 tgatctccgc agagccgttg cggccagtct tgatgaagtc gccgtcaaaa agtggttgcc
     1861 gctgccgcgc gttctcgaag ttcgagcgcc cggcccgctg cagtgcgacg tccccttcca
     1921 cgaagatgaa cgaagcatcc ccgttctcgt cccatccccc cagaacgcgt cgcgagtagg
     1981 actgggattc gacggcgagc cggacggcct cgtcgtaatg cttgtcgccc atcgcggtgc
     2041 gcgcgtcctg cagcagcccg agcgcgcggg caacgttgga accggggcgc accgtaccgg
     2101 cggagcgcgt cgctcgggtc accagggtcg aggccgtttc gatctcggcg cgggcgcggt
     2161 ccgcggctgt cggctttccc cggacgtacg cgcgatagcc gagaatgctg ccggcggcca
     2221 cgagcacgat cagcaggccc cagatcgtga ccttgcggac ggggatgacg aaccaatcga
     2281 ggtccgctcc gaccagccgg ccgagattcc gtctccgcat aactttcgtg attggcgaga
     2341 cttaccctgt gttcttatag cacaccccga agatgtcgcg gggacaggtc ctgcggacct
     2401 gcacccgcca ccttatccgt gatcccggac ctgtcccggc ggtatagtcg cgcgttccca
     2461 aaaggagcct tggaatgctc aaaaccatgc gcgccagctt ccaccatctc aagtggacgc
     2521 tgttcgcagt catcatcgtt ttcgttctcg ggttcgtctt cttctccgga tcgaactggg
     2581 gaagggacag ctcgagccag gtcgtcgccc ggatcggggc cgaagagatc tccgcggtcg
     2641 aattcgatcg catctaccag aaccaggttc agcgctaccg cgagatgtac aagggaagct
     2701 tctctcccga gctcgcccgt gcgctcgatc tgccgcggca ggtgctcgac gggatgatcg
     2761 agcggaagct ccggatcgag gcggcgaagc ggatgaacct cgcggtgacc gacgaagagc
     2821 tcgcgcgcgc catcgcggtg ctcccgttct ttcaggagaa cgggttgttc atcggcgccg
     2881 acaagtacgc cgagcggttg cggatggccg ggatggcgcc ggaggcgttc gaggaaggga
     2941 tgcgggagga cctgctcgtc gacaagcaca ccgcgctcgt caaggcgagc gtcgccgttc
     3001 tcgacgccga catccggcgg gaattctccg cgaagaacga caaggcgaag atcgagtaca
     3061 tcgtcgttcc ggcaagccgc ctcgagagcg cgctcgagcc ttcggaggcg gacctgaaga
     3121 cctactacga gaagaacaag gagcgttacc gcgctcccga gcagcgccgg gcgaactacc
     3181 ttctcgtcga ccgtttgaag gtccgcgcga aagtccccgt gaccgacgca gagatccgcg
     3241 ccgagtacga gtcgaagaaa gcctccttct cggtgccgga gcaggtgagc gtctctcaca
     3301 tcctcgttgc tgtcgattcg aagggaggac cggaggcgga cgcgcgggcg aaggcgaagg
     3361 cggaagcgct cgcggcccgc gcgaaggctg gcgaggattt cgcgaaactc gcgaaggaga
     3421 acaccgacga cccgaccggc aaggagaacg gggggcagct tcccccgttc gggcgcgggc
     3481 agatggtccc cgagttcgag caggccgctt tcgacatgaa gccgggggag atccgtg
//
LOCUS       DASWAV010000235         5053 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U2665
            k141_569727, whole genome shotgun sequence.
ACCESSION   DASWAV010000235 DASWAV010000000
VERSION     DASWAV010000235.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960316
            Sequence Read Archive: SRR10260007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 5053)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5053)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:47:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,929
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5053
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_569727"
                     /isolate="SMAG_U2665"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.36444 N 132.31889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..212
                     /locus_tag="VF958_04770"
     CDS             <1..212
                     /locus_tag="VF958_04770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9148458.1"
                     /translation="APVVVVMGTDDESVPFEHVREHWRGWEGSGRLLPGSRFVEVAGG
                     DHGLVEHVPAIAAEIVRLALEETPV"
     gene            209..967
                     /locus_tag="VF958_04775"
     CDS             209..967
                     /locus_tag="VF958_04775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9148459.1"
                     /translation="MTRAALGLFLLESAGGTMLLLLFFPPGTLGNGFFSLHGLIASAL
                     VVLALVLQPAGLPPVAAGAAAVIVVAYTLMAHAGRASAARLLLVAAAAGSGYAAVRVA
                     LVAPRLPGNAWTAMSALLGGLFFASVLLVMNLGHWYLVSRSLPFQLLARGAALFAGIA
                     IVRAVVLAAAIAANPQPEGLEALLSLDRDALFFLFRIVWGIVGPLALSWFIWRTAEMK
                     SNQAATGLLYVALVFVLIGELLSAYLTVATGFPA"
     gene            970..1428
                     /locus_tag="VF958_04780"
     CDS             970..1428
                     /locus_tag="VF958_04780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9148460.1"
                     /translation="MRARHYCPNCLRARDHAEEDSGDYSCEKCGEAYSARFGGVAPGG
                     PIERCAFCGNRAFFLQKDFDQRLGCVFIALSLGIALLVGWKVGWIWFTPVLLASVLVD
                     WIVAGRIRTVTICYRCDAEYRELPLNARHRGYDPHVAERYAKTKTVRRMR"
     gene            1320..2012
                     /locus_tag="VF958_04785"
     CDS             1320..2012
                     /locus_tag="VF958_04785"
                     /inference="COORDINATES: protein motif:HMM:NF016115.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004061 - arylformamidase activity
                     [Evidence IEA];
                     GO_process: GO:0019441 - tryptophan catabolic process to
                     kynurenine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase family protein"
                     /protein_id="HEX9148461.1"
                     /translation="MRRRVSRAPVERPAPRVRPARRGAVRENEDRPPDALKIYDISRR
                     INPRTFVWPGDVPFSYQTTSSLAGGDLYESTCFTMSSHAGTHADAPNHVVTREATIGD
                     MPLAPFVGRAQVIDLPGRGEIGPDELPPKSLRVRRALFRTRGRTFLSPLAALRLVEKG
                     LILVGTDAHSIDPPDAEDLPAHRTLLSNGIALLEHLALDAVDEGEYQLVALPLRFDDL
                     DASPVRAVLIRG"
     gene            complement(2031..3377)
                     /locus_tag="VF958_04790"
     CDS             complement(2031..3377)
                     /locus_tag="VF958_04790"
                     /EC_number="1.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005543617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Glu/Leu/Phe/Val dehydrogenase"
                     /protein_id="HEX9148462.1"
                     /translation="MRPKPRPLPAVSRVLPRPRTAPGKRGAPTLKPVEAESLNPYEIA
                     QKQFDRAADHLGLDPGVRDLLRKPKRQLIVSVPVRMDNGAIRVFQGYRVQHSIARGPS
                     KGGIRYHPGVTLDEVKALAMWMTWKCAVVNIPFGGAKGGITVDTKQLSDAENERMTRR
                     YTSEISILLGHDRDIPAPDMYTTPQTMAWIMDTFSMTKGYSTLGVVTGKPLAVGGSAG
                     RNEATAEGCFVAIEEAARRLNLPLKTATAAVQGFGNAGAVLARFLNEAGVKVVAVSDS
                     RGGVHDKRGLAMDAVFSAKEKRGSVTAARGEKISNEDILELPVDILVPAALEGVITRS
                     NAPRITAKIIAEAANGPTTPDADDILRSKGAIVIPDILANAGGVTVSYFEWVQDLYSF
                     FWESDVVRNHLERTIRKAYEDVDEMARRHDTDLRTGALILAVGRVEEATRIRGLFP"
     gene            complement(3367..4110)
                     /locus_tag="VF958_04795"
     CDS             complement(3367..4110)
                     /locus_tag="VF958_04795"
                     /inference="COORDINATES: protein motif:HMM:NF003992.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:0051775 - response to redox state [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HEX9148463.1"
                     /translation="MIQSSASRPTRDPRESVSEATAGRLSVYLRCLAALESEGKANIS
                     SHELARRFHLNSAQIRKDLATFGEFGVRGVGYGIDALRRHLRSILGIDRTRRVAIVGA
                     GRLGQALADYGGFGTEGFRIVALFDNDRARIGSRSRSGVPIHDVQRLGELVARERVEI
                     AVITVPGDSAAAVARRCWDAGLKAVLNFAPVRLDAPANAYLKNVDLRINLETLSFHIA
                     RGTEPSRELSPPPRSPHRARGSKGELHAP"
     gene            complement(4107..>5053)
                     /locus_tag="VF958_04800"
     CDS             complement(4107..>5053)
                     /locus_tag="VF958_04800"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit N"
                     /protein_id="HEX9148464.1"
                     /translation="SAFTLYGIAFLFGKAGSVRIAALSRPDLGADPLVAFALLLLLVG
                     FGFKMSLAPFHAWAPDVYQGMPTPAVTYLSVAPKGASVIVLYRVLTTVFQTGMPDRFR
                     TGIAALAILSMCLGNVVALAQRDIKRLLAYSGIAHMGYVTIALAVFGREALAAVIVYF
                     FAYVVSNAGAFAAVSALYRDETKPHPVGLLAGEGRRTPFASAVLALCLFSLAGIPATA
                     GFIGKFFVFKAAMEQGLYALAIIGVVNSLVSIGYYLKVVYVLYMREPLDDERPPALAT
                     ADRVALALCGAGVLVLGIFPAGLWNLARRAAASLPFGP"
BASE COUNT          796 a         1689 c         1688 g          880 t
ORIGIN      
        1 gagcaccggt cgtcgtcgtc atgggaacag atgacgagag cgtgcccttc gagcacgtgc
       61 gggagcactg gcgcggctgg gagggttccg gccggcttct tccgggatcc cgcttcgtcg
      121 aggttgccgg cggggatcac ggactcgtcg agcacgtgcc ggcgatcgcc gcagagattg
      181 tccgactcgc gttggaagaa actccggttt gacgcgagcc gcgctcggcc tgtttcttct
      241 cgagtccgcc ggggggacga tgctcctgct cctcttcttt cccccgggaa cgctcgggaa
      301 cggattcttt tcactccacg gactcatcgc gtcagccctc gttgttcttg ccctcgttct
      361 gcaaccggcc ggcctgccgc ccgtcgcggc cggcgccgcc gcggtgattg tcgtcgccta
      421 tacgctgatg gcgcacgccg gccgggcgtc cgccgcgcgc ctgctgctcg tcgcggccgc
      481 ggccgggtcc ggctacgcgg ccgtgcgcgt cgccctggtg gcgccccgtc tcccggggaa
      541 cgcctggacg gcgatgagcg cgctcctcgg cggcctgttc ttcgcctccg tgctcctcgt
      601 gatgaatctc ggccactggt atctggtttc gcgctcactt ccgttccagt tgctcgcccg
      661 cggggccgcg ctcttcgcgg gcatcgcgat cgtccgggcc gtcgtcctcg ccgccgcgat
      721 cgcggcgaat ccgcagcccg agggtctcga agcgctgcta tcactcgatc gtgacgcgct
      781 cttcttcctg ttccgcatcg tctggggaat cgtcggaccg ctcgcgctct cctggttcat
      841 ctggcgcacg gcagagatga aatcgaacca ggccgcgacc ggccttctct acgtggcgct
      901 cgtcttcgtt ctcatcggcg agctcctttc ggcctatctg accgtcgcga caggattccc
      961 ggcctgagtt tgcgcgcgcg ccactactgt ccgaactgtc tccgcgcgag ggaccacgcg
     1021 gaagaggact cgggcgacta cagctgcgag aagtgcgggg aggcctactc cgcgcggttc
     1081 gggggggtcg cgcccggcgg gcccatcgag cgctgcgcct tctgcggcaa ccgcgccttt
     1141 ttcctccaga aggacttcga ccagcgcctg ggatgcgttt tcatcgccct ttctctcggc
     1201 atcgcgcttc tcgtgggttg gaaagtcggc tggatctggt tcacgccggt tctgctcgcc
     1261 agcgtgctcg tcgactggat cgttgcggga cggatccgaa cggttacgat ctgctatcga
     1321 tgcgacgccg agtatcgcga gctcccgttg aacgcccggc accgcgggta cgacccgcac
     1381 gtcgcggagc ggtacgcgaa aacgaagacc gtccgccgga tgcgctgaag atctacgaca
     1441 tctcccgacg aataaacccg cgaacattcg tctggccggg ggacgtccca ttctcctatc
     1501 agacgacgtc gagtcttgcc ggcggcgacc tctacgagtc gacctgtttc acgatgagct
     1561 cccacgccgg aacccacgcg gacgcgccga accacgtcgt tacccgcgag gcgacgatcg
     1621 gggacatgcc gctcgcgccc ttcgtcgggc gcgcgcaggt catcgacctt cccgggaggg
     1681 gagagatcgg tcccgacgag cttccgccga agagcctgcg ggtccgacga gcgctgtttc
     1741 gcacgcgcgg acggaccttt ctctcacccc tggccgcgct ccgcctcgtg gagaagggac
     1801 tcattctcgt cggaaccgat gcgcactcca tcgatccgcc cgacgccgag gatctgcccg
     1861 cgcaccgcac gcttctctcg aacgggatcg ccctcctcga gcacctcgcg ctggacgcgg
     1921 tcgacgaagg ggaataccag ctcgtggcgc tccccttgag gttcgatgat ctcgacgcct
     1981 cgccggttcg ggcggtgctg atccggggct gagtcgcctc ccgcgaccct ctacggaaag
     2041 agtccgcgga tcctcgtcgc ctcctcgacg cgaccgaccg cgaggatgag cgccccggtc
     2101 cggaggtcgg tgtcgtggcg gcgtgccatt tcatcgacgt cctcgtaagc cttgcggatc
     2161 gtgcgctcga ggtggttgcg caccacatcg gattcccaga agaacgaata gagatcctga
     2221 acccactcaa agtaggagac cgtgacgcct ccggcattgg cgaggatgtc cgggatgacg
     2281 atcgctccct tgcttcgcag gatgtcgtcg gcgtccgggg tcgtcggccc gttggcggcc
     2341 tcggcgatga tcttcgccgt gatgcgcggc gcattgctcc gggtgatgac gccttcgagc
     2401 gccgcgggga cgaggatgtc cacggggagt tccaggatgt cttcgttgct gatcttctcg
     2461 ccgcgggccg cggtgacgga acccctcttc tctttcgcgg aaaacaccgc gtccatggcc
     2521 aggccccgct tgtcgtgcac tccacccctt gagtcggaga cggcgacgac tttcacgccg
     2581 gcctcgttga gaaaacgcgc gagtaccgcg ccggcgtttc cgaatccctg gaccgccgcg
     2641 gtcgccgtct tcaaagggag gttgagtcgt cgagcagcct cctcgatggc gacaaaacag
     2701 ccttccgcgg tggcctcgtt gcgcccggcc gatccgccga ccgcgagcgg cttgcccgtg
     2761 acgacgccca gcgtggaata ccccttcgtc atcgagaacg tgtccatgat ccaggccatc
     2821 gtctgcggtg tcgtgtacat gtcgggcgcg gggatgtcgc ggtcgtggcc gagtaggatc
     2881 gagatctcgg acgtataccg tcgggtcatc cgttcgttct cggcgtccga gagctgcttc
     2941 gtatccacgg tgatcccgcc cttcgcgccg ccgaacggaa tgttcacgac cgcgcacttc
     3001 cacgtcatcc acatcgcgag ggccttcact tcatccagcg tgacgccggg gtggtagcgg
     3061 atgccgccct tcgacgggcc gcgggcgatc gagtgctgga cccgatatcc ctggaagacc
     3121 cggatggctc cgttgtccat gcgaaccggg accgaaacga tgagttgccg cttcggcttc
     3181 cgcagcaggt cgcgcacgcc ggggtcgagc ccgaggtggt cggccgcccg gtcgaactgc
     3241 ttctgcgcga tttcgtacgg gttcagagac tccgcttcga ccggcttcag cgtcggggcg
     3301 ccgcgcttcc cgggagcagt cctgggccgg gggagcacgc ggctcaccgc cggaaggggc
     3361 ctgggcttag ggcgcatgca gttctccttt gcttcctctc gcgcgatgcg gcgaccgcgg
     3421 agggggagag agttcacgag aaggttcggt tccgcgcgcg atgtggaagg agagcgtttc
     3481 gaggttgatc ctgagatcga cgttcttgag atatgcattc gcgggagcat cgagacggac
     3541 cggcgcgaag ttgagaaccg ccttcagtcc ggcatcccag cagcgacgcg cgaccgccgc
     3601 cgccgagtct cccggcaccg tgatgaccgc gatctcgacg cgctcccgcg ccacgagttc
     3661 gccgagccgc tggacgtcgt ggatcggcac accggagcgg ctgcgggagc cgattcgcgc
     3721 gcggtcgttg tcgaagagcg cgacgattcg aaatccttcc gtcccgaatc cgccgtagtc
     3781 cgcgagcgcc tggccgagcc gcccggctcc cacgattgcg acgcgccggg ttcgatcgat
     3841 cccgagaatc gagcgcaggt gccgcctcaa cgcgtcgatc ccgtacccta cgccgcggac
     3901 accgaattct ccgaaggtgg cgaggtcctt gcggatctga gccgagttca ggtgaaaacg
     3961 acgcgcgagt tcgtgcgacg agatgttcgc cttgccttcc gactcgagtg cggcgaggca
     4021 tcggagataa accgaaagcc ggcccgccgt ggcctcggag acggattctc tcgggtcgcg
     4081 cgtcggtctc gaggccgagc tctggatcat ggaccgaacg gaaggcttgc cgctgcgcgg
     4141 cgcgccaggt tccaaagccc cgcggggaag atgccgagca cgagaacgcc cgccccgcac
     4201 agggcgagag cgacacggtc cgccgttgcg agagcgggcg gacgctcatc gtccagcggc
     4261 tcgcgcatgt agagaacgta cacgaccttc aggtagtaac cgatcgacac gaggctgttg
     4321 acgacgccga tgatcgcgag ggcgtagagg ccctgctcca tcgcggcctt gaacacgaaa
     4381 aacttgccga tgaagcccgc cgtcgcggga atgccggcga gcgagaagag gcataacgcg
     4441 agtaccgcgg aagcgaacgg ggtgcggcgt ccttccccgg cgagcaggcc gaccggatgg
     4501 ggcttcgttt cgtcgcggta gagagcggag acggccgcga acgctccggc gttcgagacg
     4561 acatacgcga agaaatagac gatgaccgca gcgagcgcct cgcggccgaa gaccgcgagc
     4621 gcgatggtga cgtatcccat gtgcgcgatc cccgaatagg cgaggagacg cttgatgtcg
     4681 cgctgcgcga gggcgacgac gttgccgaga cacatcgaga ggatcgccag cgcagcgatg
     4741 ccggtgcgga agcgatcggg catcccggtc tggaagaccg tggtcaggac gcgatagagc
     4801 acgatcacgc tcgccccctt cggggcgacc gagagatacg tcacggcggg ggtcggcatc
     4861 ccctggtaca cgtcgggcgc ccacgcgtga aagggcgcga gcgacatctt gaagccgaaa
     4921 ccgacgagca gcagcagcag cgcgaatgcg acgagcgggt ccgcgccgag gtccggccgc
     4981 gagagagccg cgatccgcac gctccccgcc tttccgaaga ggaaagcgat cccgtagagc
     5041 gtgaatgccg agg
//
LOCUS       DASWAV010000236         1824 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U2665
            k141_331300, whole genome shotgun sequence.
ACCESSION   DASWAV010000236 DASWAV010000000
VERSION     DASWAV010000236.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960316
            Sequence Read Archive: SRR10260007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 1824)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1824)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:47:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,929
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1824
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_331300"
                     /isolate="SMAG_U2665"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.36444 N 132.31889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..650)
                     /locus_tag="VF958_04805"
     CDS             complement(<1..650)
                     /locus_tag="VF958_04805"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX9148465.1"
                     /translation="MLSIVLPTKNEKENVGPLFQRIEEALEGVDYEVVVVDDSDDDTA
                     EIAAALGERAAYDVRVIRRGREERAGGLSAAVVRGARAASAPWVCVMDADLQHPPEVI
                     RELLLRAADGDVDVVVASRYCPSGDSNGFGRLRAFVSQTVTKSARLLFPRRLQGVSDP
                     MSGFFLLRREGIELDRLRPQGFKILLEILATHGGLRRAEIPFEFGTRYAGDSKASLR"
     gene            complement(992..1645)
                     /locus_tag="VF958_04810"
     CDS             complement(992..1645)
                     /locus_tag="VF958_04810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014374802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX9148466.1"
                     /translation="MEPRVLIADDHPPIRTAVRAVLEEYGFAVCAEAGDAQSAVDAAL
                     RERPDACVMDIYMPGGGIRAAAEISAKLPDTAILILTFSRSDEDFLAALRAGASGYLL
                     KDVAPERLPEALRSVLRGEAVIHDSLVTRLIEEVRPPRRGRVHVVDRVGADLTPRESE
                     VLELLKQEKTTGEIAYVLSISEVTVRRHISGVVKKLRVRNRKEALRVVGARSGRSDN"
     gene            complement(1645..>1824)
                     /locus_tag="VF958_04815"
     CDS             complement(1645..>1824)
                     /locus_tag="VF958_04815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006359750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX9148467.1"
                     /translation="ESGDGITLRIVDDGVGFDGEATPRGFGLRSMRERVEELGGEFRV
                     ESLSKAGTTIEAFLP"
BASE COUNT          310 a          610 c          558 g          346 t
ORIGIN      
        1 cgcagcgacg ccttgctgtc gccggcatat cgggttccga actcgaacgg gatctcggct
       61 cggcgcagac cgccgtgagt ggcgagaatc tccaggagaa tcttgaagcc ctgcgggcga
      121 aggcggtcga gctcaatccc ttcgcggcga aggaggaaga agccgctcat cgggtcactg
      181 acgccttgga ggcgccgagg aaagagcaac cgcgcagatt tcgtcaccgt ctgcgagacg
      241 aacgcacgca ggcggccgaa tccattcgag tcgccactcg ggcaataacg gctcgcgacg
      301 acgacatcga catccccgtc tgccgcacgc aagaggagtt cgcggatcac ttccggcgga
      361 tgctggagat cggcgtccat cacgcagacc cagggcgcgc tcgccgcacg agcgcccctg
      421 accacagcgg ccgagagccc tccggctcgc tcctctcgtc cgcgtcgaat cacgcggacg
      481 tcgtaggcag cgcgctcgcc gagggctgcc gcgatctcgg ccgtatcgtc gtcactgtcg
      541 tcgacgacga ccacctcgta atcgactccc tcgagcgctt cttcgatgcg ctggaagagc
      601 ggcccgacgt tctccttctc gttcttggtc ggaaggacta tcgacagcat cggcgtggct
      661 gcttcggcct gttccggctc gtcccgcgcc acggaatgtc ggggcaggcg tgcaagctct
      721 cgttctgcgt tagatgtctg acccatgtct ctcctgcccc gaaggaggcg cgaccgcgtc
      781 gcggagggct gaggtgcctc gaaccacagc aagcagcctt ggcaccctgc gtccgctctt
      841 catcgcgttc ctccttcgtt tgttgaatgt ccgcaatccg caagcgcgtg gacgccgggg
      901 cgttcacgcg ggctttgaag ctcttaacta gattcgtatc gtgaaaccgt ggtcgaatgc
      961 aatgggccag ccgacccatt tgcgttcacg ttcagttatc tgaacgtcct gaacgcgcgc
     1021 cgacgacgcg aagcgcttcc ttccgattgc gcactcgcag ctttttcacg acccctgaga
     1081 tgtgtcgccg gactgtcact tccgaaatcg aaagcacgta cgcgatctct cccgtcgtct
     1141 tctcctgttt gagcagctcg aggacctcgg attctcgcgg tgtgaggtcc gcgcccacgc
     1201 gatcgactac gtgtactcgc ccgcgccgag gggggcgcac ctcctcgatg agacgagtaa
     1261 ctaggctgtc gtggatgact gcctcgcctc tcagtacgct cctgagggct tccggaagtc
     1321 gttcgggtgc gacatccttg agcagatatc ccgatgctcc cgccctcagg gcggcaagga
     1381 aatcctcgtc gctgcgcgag aaggtcagga tcagaatcgc agtgtctgga agcttggctg
     1441 agatctcggc cgcggctcgg ataccgcctc cgggcatgta gatgtccatg acacaggcgt
     1501 ccgggcgctc gcgcagcgca gcgtcgaccg cgctctgcgc gtcgccggcc tcggcgcaca
     1561 ccgcgaagcc gtactcctcg agcactgccc ggaccgcagt acggatcggt gggtggtcgt
     1621 ccgctatgag aacccggggt tccatcaggg cagaaaggcc tcgatcgtgg tccccgcctt
     1681 cgaaagagat tcgacgcgga actctccgcc gagctcttca acgcgttctc gcatgctcct
     1741 gagcccgaat ccccgaggtg tggcctcgcc gtcgaacccg accccgtcgt cgacgatgcg
     1801 gagcgtgatt ccgtctccgg attc
//
LOCUS       DASWAV010000237         4985 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U2665
            k141_213365, whole genome shotgun sequence.
ACCESSION   DASWAV010000237 DASWAV010000000
VERSION     DASWAV010000237.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960316
            Sequence Read Archive: SRR10260007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 4985)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4985)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:47:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,929
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4985
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_213365"
                     /isolate="SMAG_U2665"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.36444 N 132.31889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..872)
                     /locus_tag="VF958_04820"
     CDS             complement(<1..872)
                     /locus_tag="VF958_04820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9148468.1"
                     /translation="MNPAVVAPPPKAMERIRRALVGGHPLLYVQSWEEARVERLAQHL
                     AKTFYGEPAAYGVWSVVEGLSVDGKPVPATNDPLAALEAILGMEGKAFYVMKDFPGAA
                     DGRPEIVRRLRDLYRQLKDKGRHVLLVSPRLVIPEDAKKEVYVIEYELPDEVEIARML
                     EANLKRRLGSDGISDAELKRLALALRGLTSDEIGHVLSKTFAQRTAFDEEAFREILAE
                     KEQMSRKEGVLEFVPPRVSLSDVGGYDVLKAWLHKRQKLFSREALDAGIPIPRGILLM
                     GISGCGKSLCVKTIS"
     gene            931..2367
                     /locus_tag="VF958_04825"
     CDS             931..2367
                     /locus_tag="VF958_04825"
                     /inference="COORDINATES: protein motif:HMM:NF024986.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HEX9148469.1"
                     /translation="MANAEHVKLVQTEEWNDWRARNPGVTPDLTAANLSGTDLSGKDL
                     RRADLTRANLSGATLAGTDLSRAMLNLARLEGASAPGAIFEQCDMDFLDLTGIFLAGA
                     RLRSASLQSCSLLNANFAGADLTLTKLEGSDLNNANLAGATLCDVNFRGVKIYGAHFD
                     GADLSRSFFEGVAAPNGAFPNANFTRANLKAADFTGADLRRAKFVGAVLRQAALNAAD
                     CERADFAEADMELVAAVEANFSGAQFDRARLRKADLRSALFLDAGGEDVSFAEARLVQ
                     VDLRTARFERANFNGADLSKAVLRRVNLKPYSFAGARFAQADASEAQAEGVDFSRADL
                     SFGNFRQANLKRANMSHVLALEADLSGTLLIGANFSGANLEQADFRFARAIGADFTRA
                     RLSGADFANADLSEAKFKKTHFWGASARRAKLPSGAGVLFSISTMFSRAKEKQDRVDD
                     HKEKARQKRIEELAAKAEKKPPHVTGKR"
     gene            complement(2485..3858)
                     /locus_tag="VF958_04830"
     CDS             complement(2485..3858)
                     /locus_tag="VF958_04830"
                     /inference="COORDINATES: protein motif:HMM:NF025598.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4340 domain-containing protein"
                     /protein_id="HEX9148470.1"
                     /translation="MSSRKLLILTGVVVVLFAFILFFERRMPTTSEREQKGDLYWDLP
                     EDQVESILLTRGGEIVELAKTGGSWRLVRPEAYSADSFAASDLASQLADLKNPGGESG
                     GEGKPEGYGLSKPSAKATIVWKDPKKPGKKQSRTLEFGIDIPGTDVTAARVPGTSQIL
                     FVPAALAASVRKPADEFKSKDVFGGASFDVASIDVARGRGRLTLAKRNGIWWLEQPIA
                     DLADRDVADRLSNDLGSLRVTEFVPRAQATDLSALGLAPPVYRITLTDAKGSRRALDL
                     GSTRSDGNSVYAAHEGQVFTIGNSIVDDLSKEAEVFRDKRLVRFERSDVSGITASAAG
                     GKPRIFARQQAGWTLGGKAMLASVADDLMTAILDVETSSFLDDAPLKAFAARPAETEL
                     EIRLAAGPPWKVSIYPFRGEFAAVVSRRPGAFGVSSDAIQRLKGAIEKAAAAPTVTPA
                     PKPTAGK"
     gene            complement(3868..>4985)
                     /locus_tag="VF958_04835"
     CDS             complement(3868..>4985)
                     /locus_tag="VF958_04835"
                     /inference="COORDINATES: protein motif:HMM:NF021351.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Gldg family protein"
                     /protein_id="HEX9148471.1"
                     /translation="RQSTVVFRSGDRKKYVEEDKLADFDFANAQMGRAAPEIKSFKGE
                     EAFTSAILAVTEARSPHVYFSAGHGEGAIDSGDRGRGFTDAKQLLERDNLVVAKWDSL
                     GKDAVPPDADAIIVAGPRTAFLEPEAGALEKYFAGGGRILLMLDPVLPAAGSPAPDYG
                     LGKLLAGNGVRLGNDIVVDPSNALPLVGAETVFANHYGSHPIVRALTDEGLPVILPLA
                     RSVAKADATAAASPTVLVETSPDGWGETSLANLEEQVKKDPQDTAGPVGLAVAVGPTE
                     GEKAPGKKGRLVAVGNSRFAANGALGNAGNANFFLNSVHWLTGSEKQIGIAPKTPEQA
                     SLSITQSQVRRIGLFSIVGLPALAVVLGVWVWYRRRD"
BASE COUNT          847 a         1682 c         1628 g          828 t
ORIGIN      
        1 gagatcgtct tgacgcagag gctcttgccg catccggaga ttcccatgag gagaatgcct
       61 cgcgggatgg ggatgcccgc gtcgagcgcc tcgcgcgaga agagtttctg acgcttgtgg
      121 agccacgcct tcaggacgtc gtaaccgccc acgtcggaga gcgagacccg cggcggcacg
      181 aactcgagga ccccctcctt gcgggacatc tgctccttct cggccaggat ctcgcggaac
      241 gcctcctcgt cgaacgcggt ccgctgcgcg aacgtcttgg agagcacgtg cccgatctcg
      301 tccgaggtca atccgcgcag agccagcgcg agccgtttca actcggcatc gctgatccca
      361 tcggacccga ggcgccgctt caggttggcc tcgagcatcc gggcgatctc gacctcgtcg
      421 ggcagctcgt actcgatgac gtagacctct ttcttggcgt cctccgggat gacgagccgg
      481 ggagacacga gaaggacgtg ccggcctttg tccttgagtt ggcgatagag gtcgcgaagt
      541 ctccgcacga tctccggacg cccatcggcg gcgccgggga agtccttcat gacgtagaag
      601 gccttcccct ccatgccgag gatggcctcc agcgcggcga gcggatcgtt cgtggccggc
      661 acgggcttgc cgtcgacgga gagtccctcg acgaccgacc agacgccgta cgccgccggc
      721 tcgccgtaga aggtcttggc gagatgctgg gccaggcgct cgacccgtgc ctcctcccag
      781 gactggacgt acaggagggg atggcccccc acgagcgcgc ggcggatacg ctccatggct
      841 ttcggcggag gagcgacgac ggcggggttc atcgaggtga tcttaccgcc ggaagccctg
      901 agccgtccgg cggttacaat cgccggacac atggcaaatg ccgaacatgt caaactggtt
      961 cagaccgagg agtggaacga ctggcgcgct cgcaatccag gagtgacccc ggacctgacc
     1021 gccgcgaacc tctccgggac cgacctgtcg gggaaggacc tgcgccgagc cgacctcacg
     1081 cgcgccaatc tttccggagc gaccctcgcg ggcaccgacc tctcgcgcgc catgctcaac
     1141 ctggcgcgcc tcgaaggcgc atccgctccg ggcgccatct tcgagcagtg cgacatggac
     1201 ttcctcgacc tgacgggcat cttcctcgcc ggtgcgcggc tccggtccgc ctctcttcag
     1261 agctgctcgt tgctcaacgc gaatttcgcc ggagcggacc tcacgctgac caaactcgag
     1321 ggatccgatc tgaacaacgc gaatctcgcg ggtgcgacgc tctgcgacgt caacttccga
     1381 ggagtaaaga tctacggcgc ccacttcgac ggcgcggacc tctcacgctc tttcttcgag
     1441 ggcgtggccg ctcccaacgg agcatttcca aacgcgaact tcacgcgcgc caacctgaag
     1501 gccgccgact tcaccggcgc ggacctgcgc cgggcgaaat tcgtcggcgc ggtcctgagg
     1561 caggccgcgt tgaacgccgc cgactgcgag cgcgcggact tcgcggaagc cgacatggag
     1621 ctcgtggccg cggtggaggc gaatttcagc ggcgcgcagt tcgatcgcgc ccgcctgcgc
     1681 aaggcggacc tgcgaagcgc tctgttcctc gacgcgggag gcgaggacgt gagcttcgcg
     1741 gaagccaggc tcgtgcaagt ggacctgcgg accgcgcgtt tcgagcgcgc gaacttcaac
     1801 ggcgcggacc tttcaaaagc ggtcctgcgg cgggtgaacc tgaagccgta ctccttcgcg
     1861 ggcgcccgct tcgcccaggc cgacgcctcg gaagcccagg ccgaaggggt ggacttttcg
     1921 cgggccgacc tttcattcgg gaacttccgc caggcgaacc tgaaacgggc gaacatgtcg
     1981 cacgtactcg cgctcgaggc ggatctttcg ggcacgctgc tcatcggagc aaacttctca
     2041 ggcgccaacc tcgagcaggc ggactttcgc ttcgcgcgcg cgatcggggc agacttcacg
     2101 agagcgcggc tctccggtgc ggacttcgcg aatgccgatt tgtccgaagc gaagttcaag
     2161 aagacgcatt tctggggggc gtcggcgcgc cgcgccaagc tgccgtccgg tgcgggcgtt
     2221 ctcttctcga tctcgacgat gttttccagg gccaaagaga aacaggatcg ggtcgacgat
     2281 cacaaggaaa aggcccgcca gaaacggatc gaggagctgg cggcgaaggc cgagaagaag
     2341 ccgccgcacg tgaccggaaa aagatagcgg ggtcaggtct tcacgagtcg aggaatcgct
     2401 gaatcgagat cagcggtctg accctcttgc gaagcaagtg gccactttgg gggtcaggtc
     2461 tccactcgcc acatctgggg tcgattattt cccggctgtc ggcttcggcg ccggcgtcac
     2521 tgtgggagcc gccgcggctt tttcgatcgc acccttgagc ctttggatcg cgtcgctcga
     2581 cacgccgaac gcgcccggcc gacgcgagac gaccgccgcg aactcgcctc gaaacggata
     2641 gatcgagacc ttccacggcg gtcccgcagc caggcggatc tcgagctccg tctcagcggg
     2701 gcgcgccgcg aatgccttga gcggagcgtc gtcgagaaac gagctggtct ccacgtccag
     2761 gatcgccgtc atcaggtcgt cggcgaccga ggcgagcatc gcttttccgc cgagagtcca
     2821 gcccgcctgt tgccgggcga agatgcgcgg cttccctccc gccgccgagg ccgtgatccc
     2881 cgatacgtcg gaccgctcga accggaccag ccgcttgtcg cgaaacacct cggcttcctt
     2941 cgagaggtcg tcgacgatcg agttgccgat cgtgaatacc tgcccctcgt gggcggcata
     3001 gaccgagttc ccgtccgagc gcgtcgagcc gaggtcgagg gcccgccggg accccttcgc
     3061 gtcggtcaga gtgatccgat agacgggcgg cgccagcccg agagccgaga ggtcggtcgc
     3121 ctgggctcgc gggacgaact cggtcacccg cagcgagccg aggtcgttcg acagccggtc
     3181 cgcgacgtcg cggtcagcga ggtcggcgat cggctgctcg agccaccaga ttccgttcct
     3241 cttcgccagc gtgagccggc ccctcccccg cgcgacgtcg atcgaagcga cgtcgaaaga
     3301 cgctccgcca aacacgtcct tgctcttgaa ttcatccgcc ggcttacgaa ccgaggccgc
     3361 cagcgccgcg gggacgaaca ggatctgcga cgtgccgggg actcgggcgg ccgtcacgtc
     3421 ggttcccgga atatcgatgc cgaactcgag cgtgcgcgat tgcttcttgc ccggcttctt
     3481 cgggtctttc cagacgatcg tcgccttcgc cgagggcttc gagagcccgt acccctccgg
     3541 ctttccctcg cctcccgatt cgccccccgg gttcttcaaa tcggcgagct gcgacgcgag
     3601 gtcggacgcc gcgaacgaat ctgcggaata cgcctcgggg cgaacgagcc tccacgaacc
     3661 tcccgtcttt gcgagctcga cgatctcccc tcctcgcgtc aggaggatcg attcgacctg
     3721 gtcctcgggc aggtcccagt acaggtcccc cttctgttcc ctttccgacg tcgtcggcat
     3781 ccgacgctcg aaaaacagga tgaaggcgaa aagaacgacg acgacgccgg tcaggatgag
     3841 gagcttgcgg gaggacatgg gatcgaacta gtcccgccgg cggtaccaga cccagacacc
     3901 gagcacgacg gccagcgccg gaagaccgac gatcgagaag agaccgatcc ggcgcacctg
     3961 tgactgcgtg atcgaaaggg aagcctgctc cggcgtcttg ggcgcgatgc cgatctgttt
     4021 ctccgacccg gtgagccagt gaaccgagtt cagaaagaag ttcgcgttgc cggcgttgcc
     4081 gagcgcgccg ttcgccgcga agcgggagtt tccgaccgca acgagacggc ccttcttacc
     4141 gggcgccttt tccccttcgg tcgggccgac cgcgacggcg agtccgaccg ggcccgccgt
     4201 gtcctgcgga tccttcttca cctgttcctc gaggttcgcg agagaggtct cgccccatcc
     4261 gtccggagac gtttcgacga ggacggtcgg tgacgccgcc gcggtcgcat ccgctttcgc
     4321 gacggagcgc gcgagcggca ggatcaccgg cagcccttcg tcggtgagag cgcggacgat
     4381 cgggtggctc ccgtagtggt tcgcgaaaac ggtctcggcg cccacgagcg gcaaggcgtt
     4441 ggacgggtcg acgacgatgt cgttgccgag tctgacgccg ttgccagcca gaagcttccc
     4501 gagaccgtag tcgggcgcgg gagatccggc cgccggcaac acgggatcga gcatcaacag
     4561 gatgcggccg cctccggcga agtatttctc gagcgcgccc gcttctggct cgaggaacgc
     4621 agtccgcgga ccggcgacga tgatcgcgtc ggcgtcgggc ggcacggcgt ccttgccgag
     4681 cgagtcccac ttcgcgacga ccaggttgtc tcgctcgagc agctgctttg cgtccgtgaa
     4741 gccgcgaccg cgatcccccg agtcgatcgc gccctcaccg tggccggcag agaagtagac
     4801 gtgcggcgaa cgtgcctcgg tcactgcgag gatcgccgac gtgaacgctt cctccccctt
     4861 gaaggacttg atctccggag cggcgcgtcc catctgcgcg ttcgcgaagt cgaagtccgc
     4921 gagcttgtcc tcctcgacgt acttcttccg gtcgcccgaa cggaagacca ccgtcgactg
     4981 gcgga
//
LOCUS       DASWAV010000238        14857 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U2665
            k141_613112, whole genome shotgun sequence.
ACCESSION   DASWAV010000238 DASWAV010000000
VERSION     DASWAV010000238.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960316
            Sequence Read Archive: SRR10260007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 14857)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14857)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:47:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,929
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14857
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_613112"
                     /isolate="SMAG_U2665"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.36444 N 132.31889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(81..1847)
                     /gene="aspS"
                     /locus_tag="VF958_04840"
     CDS             complement(81..1847)
                     /gene="aspS"
                     /locus_tag="VF958_04840"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007506374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HEX9148472.1"
                     /translation="MKRQPAGVLSAADAGKKVLLRGWVGRRRDLGELIFLNVRDRSGL
                     VQVLFERVRSGEEAVARAADARAEDVVEIEGEVALRGPEQINREIPTGEIEVVATRIE
                     FLARAETPPFFPEDRTNATEELRLQYRYVDLRRPVMLKNFVLRDEIAFRVRRTLHERG
                     FLEVETPMLTKSTPEGARDYLVPSRVHRGKFYALPQSPQLFKQILMVSGFERYFQIAR
                     CFRDEDLRADRQPEFTQIDLEMSFATEDDVFEVVEAVLASAFGAAGIEAATPFPRLTY
                     AEAMERYGTDRPDLRYGLPLRDLGAAALGSGFAPFEKALAEGGSVRGLRVPGGAVLSR
                     KKLEEWTELARFNGASGLVWGRRTGGETSSPAKKALGDAGIERLFSAAEVGDGDLLLA
                     VADRPAAVSAALAALRGAAARETGAADLSKHAFCWVTEFPLFGREEKTGAWFPMNHPF
                     TSPRDEDLELLETDPGRARARAYDVVMDGEELGSGSIRIHRADVQERVFRALGISESE
                     GRERFGFLLEALKFGAPPHGGIALGLDRICMIAAGASSLRDVIAFPKTTSGSDLMTGS
                     PSSVEPAQLDELGIAPAPPPEP"
     gene            complement(1844..3139)
                     /gene="hisS"
                     /locus_tag="VF958_04845"
     CDS             complement(1844..3139)
                     /gene="hisS"
                     /locus_tag="VF958_04845"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015897438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004821 - histidine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006427 - histidyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HEX9148473.1"
                     /translation="MTPRYRSVKGTRDLVPPESERWAEAERIARETFRSRGYGEIRTP
                     VLEDTALFARSVGETTDIVHKEMYTFTDRKGRSVTMRPENTAGVVRAIVENGLASGPM
                     PLRLYYIGPQFRYERPQAGRYREFSQIGVELFGVPGPAGEAEVLEMLFAFLEELGFRA
                     LSVLLNSVGTAQSRAAFGEAVRAHVAARSGSFGEDDRRKLADNPLRLFDSKDPEVRAA
                     LEGSPRTLDFLDAESRRHHDEVRALLTSSGIPFRDEPLLVRGLDYYTRTVFEVVSADL
                     GAQDAILGGGRYDGLVESLGGPALPAIGFAIGEDRLLQVAPLAAPRRACVVVLPQSGD
                     DVADASRIAREIRAALPGGCVEVDLVARGLKKGLARASALFEEADVRGFDRNSMLAVF
                     VGERERAAGAVTVKRLATGEQETFPRSEIAERLGAARER"
     gene            complement(3136..3675)
                     /locus_tag="VF958_04850"
     CDS             complement(3136..3675)
                     /locus_tag="VF958_04850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019119782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HEX9148474.1"
                     /translation="MGLIREYAGKMPRLGARVYLAETAAVIGDVVLGDDVSVWYNSVV
                     RGDCHYIRIGDRTNIQDNCTVHVLHEKLPTILEEEVTLGHGAIVHSAVVRKGALIGIR
                     ATVLDGAEVGESALVGAGALVVPRTVVPPRTLWLGSPARQVRELSDAEVEDLKHFHRN
                     YLLYKEDYFRIDGRWAKSE"
     gene            complement(3700..4329)
                     /locus_tag="VF958_04855"
     CDS             complement(3700..4329)
                     /locus_tag="VF958_04855"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: protein motif:HMM:TIGR00057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HEX9148475.1"
                     /translation="MSPPPRIGLVELLDSPRELASFRNLLSTGGVAAIPTETFYALAA
                     DPTNERGVTRIFEIKGRGDGKPLLVLFASRGQLEQIGLAARPEVLDRFCRLWPAPLTV
                     ALPLASAIPASRGARSLAVRIPASDPVRALVEAVGPLTGTSANRSGAPPLVDPEAVAA
                     ALGSEIDLLVDGGVTAGGEPSTVLDATREPPVVIRTGAFRWPPEESGTR"
     gene            complement(4326..5345)
                     /locus_tag="VF958_04860"
     CDS             complement(4326..5345)
                     /locus_tag="VF958_04860"
                     /inference="COORDINATES: protein motif:HMM:NF013094.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ThiF family adenylyltransferase"
                     /protein_id="HEX9148476.1"
                     /translation="MNERHSRSVLFPGIGPEGQERIARFSLALVGVGAVGAAAAEMAA
                     RAGVGRLTLIDRDVVEESNLSRQLLFDASDAAAVVPKASAAEARLSEIAPDVSVRGIV
                     ADLAPDNARDLLGGHDLVLDGSDNFETRLLVSDAARSLGLPSIYAACVGEEGLLAVSV
                     PGRTPCLRCYLEVLPPPGSAPTCDTAGVVPTLPVLVASLAVTEALRIATGAEPSRGVL
                     AIGVWRVGFRSTRSFENVRPRNDCPVCSHGSFPALEAGAAEIVKLCGRRSVQVAPPAR
                     ERPDFDALEERLARVGRVRRSPQLLNADLEDVSLTIFSDGRCVVRGTEDPDRARRLYD
                     RFVGR"
     gene            complement(5342..7015)
                     /gene="recN"
                     /locus_tag="VF958_04865"
     CDS             complement(5342..7015)
                     /gene="recN"
                     /locus_tag="VF958_04865"
                     /inference="COORDINATES: protein motif:HMM:TIGR00634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HEX9148477.1"
                     /translation="MLRRLTVRNLAIVEDLDLELGPGLTVVTGETGAGKSILVDALAL
                     LAGGRGASDLVRQGADRLVVAGEFDCDRSLSEVLVEAGLPADGETLLVRRELSPDGRG
                     RAFIEDEPAAVRTLARLGERLVSIQGQSSEKELVDPEAPLELLDAYAGAGSEREAVAR
                     AASVWAAARETVERLEASRRDRSGRLDVVEFQIREIEAASPNEREEEELTYERERLRH
                     ADRLRRAGESALEALSEGESSAADRLGEASRAFAELAAIDPRESGRLEELEDLKRRVT
                     DLAAAARDAASGIEADPDRLTSVESRLEKLSRLKHKYGPTLADARALLEKLVIERDEL
                     GNFEDTLDRRQREERDARDAYRSAASALSAKRQGAAPRFSATVEKELGGLALEKSRFR
                     IALTPTGDEEPRRTGLERASFLFQPNPGEAERPIERIASGGELSRLQLAVHSAGAARA
                     GRRRTLVFDEVDAGIGGRTAESVGRKLRRLAERDQVLCVTHVPQIAALADRHFLAEKR
                     EARGRTVAAVRILEGKERVAEIARMIAGEKVPETAMRHARELLSQAGNA"
     gene            complement(7023..7754)
                     /locus_tag="VF958_04870"
     CDS             complement(7023..7754)
                     /locus_tag="VF958_04870"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HEX9148478.1"
                     /translation="MEELAGKLDDSRLDRNVVVREALTHLYLGREVSWEQAIGDNRSP
                     LAIRALLATFDPRNATLEAEYYREIDLAKWAPVKPLLWFWQMFDHSPVGRNVRLGTQM
                     RAMLAPRVFRRCGRDVRIFHGVEFSFGYNLSVGNGVTIHRNVLIDDRGEVVIGDDASI
                     SDYVNIFSHSHAVEDIHDVSLGRTVIGDRARITYHAVIFSGVEIGADALVGSMGVASK
                     PVADGVIAGGIPARPIGQKKRHTQS"
     gene            complement(7846..9351)
                     /locus_tag="VF958_04875"
     CDS             complement(7846..9351)
                     /locus_tag="VF958_04875"
                     /EC_number="3.4.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF013079.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl aminopeptidase"
                     /protein_id="HEX9148479.1"
                     /translation="MRLKIVSTLSRSAAFWRVAFVHEGRDAGRGFPPALAGSIRKAAR
                     ASSFRGRDRETAGAAGDDGSWLLVGLGRAPASLARLRRALRRALGVNASRSRSRLLLA
                     FGAGIDGSTLRALLPHLALGDYAFERYKSARKKVRPPGETTAVVVPPEGVDVAELSAA
                     AREAQVVGEAVSWARDLGNTPANDLGPADLAREAVAAGKRHGFAVRVLGRRAIAAEKM
                     AGLLAVNAGSVRPPAFVVADYRPRNPRGTAVLVGKGITFDSGGISIKPAPSMGEMKYD
                     MMGAATALACVRAAKSLRLPVRVVALTPLTENLPSGSATRPGDILRMRNGKSVEVDNT
                     DAEGRLVLADALTYSAKLRPDVLLDYATLTGAVIVALGHECAALFTGDDRLATSLLSA
                     GETTGERLWRLPLWEDYRENLRSEWADMKNTGGRPGGTINAAVFLEEFVPAGVPWAHL
                     DLAGVAHFEKEHAGYAAGATGFGVALTMEFLRKWFGGGAARAPRERLRPRV"
     gene            9422..10324
                     /locus_tag="VF958_04880"
     CDS             9422..10324
                     /locus_tag="VF958_04880"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002644431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004639 -
                     phosphoribosylaminoimidazolesuccinocarboxamide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HEX9148480.1"
                     /translation="MALATVTETHLAGARLSRRGKVRELYDLGDSLLLVASDRISAYD
                     CVLSPGIPDKGRILTQLSTFWFRKFDDVENHLIEADVSRFPEPLQADAPLLAGRSVIV
                     KKAEVIPFECVARGYLAGSGWAEYRKTGEVCGVRLPPGLSEAERLAEPIFTPATKSAH
                     GHDENVPFSRVVNDLGEEEAILIRDLTLSLYERAREYAESRGLLLADTKLEFGRLDGR
                     LLWIDEAFTPDSSRYWDAHAWTPGRTPVSFDKQFVRDWLDSTGWDHRPPAPRLPEGVV
                     RTTREKYLEAYRTLTGSLPAYLSS"
     gene            10425..11072
                     /locus_tag="VF958_04885"
     CDS             10425..11072
                     /locus_tag="VF958_04885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0009986 - cell surface [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HEX9148481.1"
                     /translation="MPNPLRLITLVLAVGAFAAVAQAQNADYNTGGPTANTLQLKLVE
                     PAEGATITGNQVRVGVSYNTHNFGEGQGTKFGEANFPQPTFDIFLDNDLRKTVKGTEA
                     NVVFLDNVPAGAHKIVVMAKNVSGEVIDRKQVNITTVAAVAEAAPAPATTTYEARTAP
                     PPPPAPVIEAPAPPPAPPAPMERETLPKTASSYPTLAVAGFALVAVGLSLGRKVS"
     gene            11324..11632
                     /locus_tag="VF958_04890"
                     /pseudo
     CDS             11324..11632
                     /locus_tag="VF958_04890"
                     /inference="COORDINATES: protein motif:HMM:NF016119.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sortase"
     gene            complement(11635..13056)
                     /gene="radA"
                     /locus_tag="VF958_04895"
     CDS             complement(11635..13056)
                     /gene="radA"
                     /locus_tag="VF958_04895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019015533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HEX9148482.1"
                     /translation="MSPKPTSVFACQSCGATSSKWLGRCPDCAEWNTYVEETRAAPTT
                     APSGPPRSRPLSEASAAAGARLETGLPGFDRVLGGGLVAGSVVLVGGEPGIGKSTLLL
                     QAAHALATDSRAVLYAAAEESVDQVRLRADRLGVGHEKLLVLSDTDVERIVAESSAGE
                     RSAVVVDSIQAVRNAALSSAAGTVSQVRESAAALVRFAKERAVPVLLVGHVTKDGSLA
                     GPRSLEHLVDAVIAVDGDRGSSRRILRATKNRFGSVDEVALYEMTARGLAEVPNASAA
                     LLAERRPGLPGSAVTATREGTRSLLVEIQALVGPATAGSPRRVVIGVDSGRLSLLLAV
                     LERAGIPLSSREVFVSCAGGLQIEEPGADLAIVAALISSARGHALPDSAVFFGEIGLL
                     GEVRRVPAAASRVREAAALGFGRIYLPSGNASETAASASVALTPVDRVAELISELAGA
                     RPGGEGSARMASRSGAGPTESAG"
     gene            complement(13149..14600)
                     /locus_tag="VF958_04900"
     CDS             complement(13149..14600)
                     /locus_tag="VF958_04900"
                     /inference="COORDINATES: protein motif:HMM:NF014523.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MlaD family protein"
                     /protein_id="HEX9148483.1"
                     /translation="MNTAAKVGAFFLVVLAIIALFIWKIEDLRIGRGGAPRKVTVQFK
                     NVAGLDEKTAVRVAGVRVGRVSKIRLTGAGRALVDVELDHDVALRQGASASIESLGLL
                     GEKYVELVPGPFGAPPLPDNAVIVGGMPVSFDEITKLARDIGVDVKEITQSLKQSIGG
                     PGGEERLENIVENMRAISEQIRGMVESNRAGVDATVANFREFSTAMKELVARVDSVVA
                     ANQGNVTQGLSNIREVSGKLETTADNLNQITGRIKEGEGTVGKLVQSDETHKNINDAL
                     VAVKEGLASFNKAIGALGKTKIDLGMRAEHLAAHNRGQGYFTLDIQPADTARFYRIEL
                     ATQPFGRRKSSTTVERTTFPDGHTEQTTTETDEFKDDFAISALFGFRMNDLVARGGLI
                     EGRGGAGVDYMMLKDRLRFSADVWDFNRPGDFSAHAKLAGRYYFSPSVFVTGGWDDFL
                     NRRRNADSLFIGAGLRWSDDDIKYLMGSIPLKR"
     gene            complement(14597..>14857)
                     /locus_tag="VF958_04905"
     CDS             complement(14597..>14857)
                     /locus_tag="VF958_04905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX9148484.1"
                     /translation="IDEVILELKERLDVTIVTITHHMESARKIGDRVALIHGGRLLYQ
                     ASPEEFLRSDDPAVRQFVEGRAEGPLTVEFDRKSESARLEGA"
BASE COUNT         2382 a         5271 c         4973 g         2231 t
ORIGIN      
        1 gcgcggatcg tcgagagctc cgcggcccac ccagacgacg cttgcggtgt cgccgacgcg
       61 gacctcgatc cgctcggggt tcacggttca ggaggggggg ccggagcgat cccgagttcg
      121 tcgagctgcg ccggctcgac cgatgacggg gacccggtca tcaggtcgct tcccgacgtg
      181 gtcttcggga aagcgatcac gtcgcgcaga gacgacgcac ccgccgcgat catgcagatc
      241 cggtcgagac cgagggcgat tccgccgtgc gggggtgcgc cgaacttgag cgcctcgagg
      301 agaaacccga atcgctcgcg cccttccgac tcggagatgc cgagagcgcg aaagacacgc
      361 tcctgcacgt cggcgcggtg gatgcggatg gagcccgagc cgagctcctc gccgtccatg
      421 acgacgtcgt acgcccgcgc ccgcgcccgt cccggatccg tctcgaggag ctcgaggtcc
      481 tcgtcgcgcg gcgaagtgaa agggtggttc atcgggaacc acgcgccggt cttttcctcg
      541 cgcccgaaca gggggaactc cgtcacccag cagaaagcgt gcttcgagag gtcggccgct
      601 ccggtctccc gcgcagccgc accccgcaga gccgcgagcg ccgccgatac cgccgccggc
      661 cgatcggcga cggcgagcag aaggtcgcca tcaccgacct ccgccgcgga gaacaggcgc
      721 tcgattccgg cgtccccgag cgccttcttc gccggagagc tcgtctcgcc cccggtacgc
      781 cggccccaga cgaggcccga cgcgccgttg aaccgcgcga gttcggtcca ctcctcgagc
      841 ttcttgcgcg agagcactgc gcctccgggg acccgaagcc cgcgcacgga gccgccttcg
      901 gcgagcgcct tctcgaaggg agcaaagccc gatccgagag ccgccgcgcc gaggtcgcgg
      961 agcggcagcc cgtagcgaag gtcaggtcgg tccgtcccgt agcgctccat cgcttcggcg
     1021 taggtgaggc gcggaaacgg cgtcgccgcc tcgatgcccg ccgcgccgaa cgcggaggcg
     1081 agaaccgcct cgacgacctc gaagacgtcg tcctcggtcg cgaaggacat ctcgagatcg
     1141 atctgcgtga actccggctg gcggtctgcc cggaggtcct cgtcgcggaa gcagcgagcg
     1201 atctgaaagt accgctcgaa gcccgacacc atcaggatct gcttgaagag ctgcggagac
     1261 tgcggcagcg cgtagaactt cccgcgatgg acgcgtgacg ggacgaggta atcacgcgcc
     1321 ccctcgggcg tcgatttcgt caacatcggc gtctcgacct cgagaaagcc ccgttcatgg
     1381 agcgtccgcc ggacgcggaa ggcgatctcg tcgcggagaa cgaaattctt gagcatcacc
     1441 gggcgccgaa gatcgacgta acggtattga agccgaagct cctccgtcgc gttcgtgcgg
     1501 tcctccggaa aaaacggggg ggtttcggcg cgagcgagga attcgattcg cgtcgccacg
     1561 acctcgatct cgcccgtcgg gatttcacgg ttgatctgtt ccggcccccg caacgcgact
     1621 tcgccctcga tctcgacgac gtcctccgcc cgcgcatcgg cggcgcgcgc gacggcctcc
     1681 tcgcccgatc ggacccgctc gaagagcacc tggacgagcc cgctccggtc gcgcacgttc
     1741 aggaagatga gctctcccag gtcgcgccgc cggccgaccc acccgcgcag gagcaccttc
     1801 ttccccgcat ccgccgcgga aagaacgccc gccggctggc gcttcaccgc tccctcgcgg
     1861 cgccgagacg ctcggcgatc tcggagcgcg ggaaggtctc ctgctcgccc gtcgcgaggc
     1921 gcttgaccgt cacggcgccg gcggcgcgct cccgttcgcc gacgaagacg gcgagcatcg
     1981 agtttcgatc gaatccccgc acgtccgcct cctcgaagag cgcgctcgcg cgagccagtc
     2041 ccttcttgag ccctcgcgcg acgagatcga cttccacgca tccgcccggc agcgcggcgc
     2101 ggatctcccg ggcgatgcgg gacgcatcgg ccacgtcgtc cccgctctga gggagaacca
     2161 ccacgcaggc gcggcgcggg gcggcgagcg gcgcgacctg gagcaggcgg tcctcgccga
     2221 tcgcgaaccc gatcgcggga agagcggggc cgccgagcga ctcgacgaga ccgtcgtagc
     2281 gccctccccc caggatcgcg tcctgggcgc cgagatccgc ggagaccacc tcgaacacgg
     2341 tccgcgtgta gtagtcgagc ccgcgaacga gcaacggttc gtcacggaac ggaatcccgg
     2401 agctggtgag gagggcgcgt acctcgtcgt ggtgccgccg ggactccgca tcgagaaagt
     2461 cgagcgtccg aggcgagccc tcgagcgccg cgcgcacctc cggatccttc gagtcgaaga
     2521 ggcggagcgg attgtcggcg agcttgcgcc ggtcgtcctc gccgaaagag cccgatcggg
     2581 ctgcaacgtg cgcgcggacc gcctccccga atgccgcgcg gctctgcgcc gtcccaacgg
     2641 agttgagcag gacggagaga gcgcggaagc cgagctcctc gagaaacgcg aagagcatct
     2701 cgaggacctc cgcctcgccc gccgggccgg ggacgccgaa gagctcgacc ccgatctgcg
     2761 agaactcccg gtagcgcccc gcctgcggac gctcatagcg aaattgcggg ccgatgtaat
     2821 agagccgcag cggcatcgga cccgacgcga ggccgttctc gacgatcgcg cggacgactc
     2881 cggcagtgtt ctccgggcgc atcgtcaccg aacgaccctt ccggtcggtg aacgtgtaca
     2941 tctccttgtg gacgatgtcg gtcgtctccc ccacggagcg cgcgaagaga gccgtatcct
     3001 cgagcactgg cgtgcggatt tcgccgtaac cccgggaacg aaaggtctcc cgcgcgatcc
     3061 gctccgcctc cgcccagcgc tcgctctcgg gcggaacgag gtcgcgcgtc cctttgacgg
     3121 atcggtatcg gggtgtcatt ccgacttcgc ccaccggccg tcgatccgga agtagtcctc
     3181 tttgtagagg aggtagttcc gatggaagtg cttcaggtcc tcgacctccg cgtccgacag
     3241 ctcgcgcacc tgccgagccg gggatcccag ccagagtgtc cgcggaggta cgaccgtgcg
     3301 tggaacgacc agggcgccgg cgccgacgag cgccgactcg ccgacctcgg ctccgtccag
     3361 gacggtggcc cggatcccga tcaaagcgcc cttgcgcacg accgcactgt ggacgatggc
     3421 gccgtggccg agcgtgacct cctcctcgag aatcgtcggg agtttctcgt ggaggacgtg
     3481 gacggtgcag ttgtcctgga tgttcgtgcg gtcgccgatc cggatgtagt ggcagtcgcc
     3541 gcggacgacc gagttgtacc agacggacac gtcgtccccg aggacgacgt ccccgatcac
     3601 cgccgcggtc tcggcgagat aaaccctcgc gccgagacgc ggcatcttcc ctgcgtactc
     3661 ccggatgagg cccatgaaag cgggcaatct agcacggcgt taccgggttc cggattcctc
     3721 gggcggccag cgaaacgcgc cggtccggat cacgacgggg ggctcgcgcg tcgcgtcgag
     3781 cactgtcgag ggctcgccgc ccgcagtcac gccgccatcg accagcagat cgatctcgga
     3841 cccgagcgcg gcggcgaccg cttcgggatc cacgagcggc ggcgctcccg accggttcgc
     3901 gctcgtcccc gtcagcggcc cgaccgcctc gaccagggcc cggacggggt cggaggccgg
     3961 gatcctcacg gcgagcgaac gtgcgcctcg tgaggccggg atcgccgatg cgagcgggag
     4021 cgcgaccgtc aacggggcgg gccagagccg gcagaatcga tccaggacct ccgggcgcgc
     4081 cgcgagcccg atctgctcga gctgtccgcg cgaggcgaag agcacgagga ggggctttcc
     4141 atcgccgcgc cccttgatct cgaaaatccg tgtcacgccg cgctcattcg tcggatctgc
     4201 cgcgagcgcg tagaacgtct cagtcgggat cgcagcgact ccgccagtcg agaggaggtt
     4261 gcggaagctc gcgagctccc ggggcgaatc gagaagctcg acgaggccga tgcggggcgg
     4321 cggactcacc gccccacgaa gcgatcgtag agacgccgtg cgcgatcggg atcttcggtt
     4381 ccgcgcacga cgcaccgacc gtcggaaaag atcgtgagcg acacgtcctc gaggtccgcg
     4441 ttcaggagct gcggcgagcg gcgtacgcgg ccgacgcgcg ccagccgttc ctcgagtgca
     4501 tcgaagtcgg gccgctcgcg cgcaggtggc gcgacctgaa cggaccgtcg gccgcagagc
     4561 ttcacgatct cggcggctcc cgcttcgagg gcggggaacg acccgtgcga gcagaccgga
     4621 cagtcgtttc gcggccggac gttctcgaac gaccgcgtcg agcgaaaccc gacccgccag
     4681 acgccgatcg cgagaactcc gcgcgagggc tcggcgcccg tcgcgatgcg cagagcctcg
     4741 gtcacggcga gcgaggccac gagcaccggc agcgtcggca cgacccccgc ggtgtcgcac
     4801 gtgggcgccg aaccgggcgg aggcagaacc tccaggtagc atcgcaggca gggcgtgcgg
     4861 cccggaaccg acacggcgag aagcccctcc tccccgacgc aggccgcata aatcgacgga
     4921 agaccgagcg accgcgccgc gtccgagacc aggagcctcg tctcgaagtt gtccgagccg
     4981 tcgaggacga ggtcgtgccc cccgagaagg tcgcgcgcgt tgtcgggagc gaggtcggcg
     5041 acgatccccc gcacggagac gtcgggagcg atctcggaga gccgcgcttc cgcggcgctc
     5101 gccttcggga cgacggcagc cgcgtccgag gcgtcgaaga gcagttgccg cgagagattc
     5161 gactcttcga cgacgtcgcg atcgatcagc gtgagccgcc cgacaccggc acgcgcggcc
     5221 atctccgccg ccgcagcacc cacggcgccc acgcccacga gtgccaggga gaaacgagcg
     5281 atcctttcct ggccttccgg accgatgccg gggaaaagaa ccgagcgcga gtgccgctcg
     5341 ttcatgcgtt gcccgcctgg gagagcagtt cgcgggcatg tcgcatcgcg gtctccggga
     5401 ccttttctcc ggcgatcatg cgcgcgatct ccgccacgcg ctccttgcct tcgagaatcc
     5461 gcaccgcggc gaccgtgcga ccgcgcgcct ccctcttttc ggcgaggaag tgccggtcgg
     5521 cgagggccgc gatctgcggg acgtgagtga cgcagagcac ctgatcgcgc tccgcgagcc
     5581 ttcggagctt ccgtccgacc gattccgccg tccggccccc gatcccggcg tcgacctcgt
     5641 cgaacacgag cgtgcggcgg cgaccggcgc gtgccgcgcc cgcggaatgc acggcgagct
     5701 gcagccgcga cagctcgccg cccgatgcga tccgttcgat cggccgctcg gcctcgcccg
     5761 ggttcggctg aaagaggaac gacgcgcgtt cgagccccgt gcgtcgcggc tcttcgtccc
     5821 ccgtcggcgt cagggcaatg cgaaagcggg acttctcgag ggcgaggccg ccgagctcct
     5881 tttcgaccgt ggcggaaaac cggggggcag cgccctgccg cttcgcggag agagccgacg
     5941 cggcggagcg gtaggcgtcg cgcgcgtcgc gctcctcccg ctgccgccgg tcgagcgtgt
     6001 cttcgaagtt gccgagctcg tctcgctcga tgacgagctt ctcgagcagc gcgcgggcgt
     6061 cggccagcgt cggcccgtac ttgtgtttca gccgtgacag cttttcgagc ctcgactcga
     6121 cggaggtcag ccgatccgga tccgcctcga tacccgaggc tgcgtcgcgg gctgctgcgg
     6181 cgagatcggt gacgcgccgc ttcagatctt cgagctcctc gagccggccg gactcgcgcg
     6241 gatcgattgc cgcgagctcg gcgaaagcac gcgacgcctc gccgagccgg tcggcggccg
     6301 aactctcccc ctccgacagc gcttcgagcg ccgattcgcc ggcgcggcgg agacggtcgg
     6361 cgtgacgcag cctctcgcgc tcataggtga gctcttcctc ctccctctcg ttgggggaag
     6421 ccgcctcgat ctcacggatc tggaactcca caacgtcgag ccggccggac cggtcgcggc
     6481 gcgatgcctc gagcctctcc acggtctcgc gggcggcggc ccagacggag gcggctcgcg
     6541 cgacggcttc gcgctccgat ccggcacccg cgtaggcgtc cagcagctcg aggggcgctt
     6601 ccggatcgac gagctctttt tcggagctct gaccctggat ggagacgaga cgctcaccga
     6661 gccgcgcgag cgtgcgcacg gcggcgggct cgtcctcgat gaacgcgcgg ccgcgcccgt
     6721 ccggcgagag ctcgcgccgg accaggaggg tttcgccgtc ggccggaagt cccgcttcga
     6781 cgagcacctc ggagagcgac cgatcgcaat cgaactcccc ggcgacgacg agccggtcgg
     6841 cgccctgccg cacgagatcg gaggcgccgc ggccacccgc caggagcgcg agggcatcga
     6901 cgagaatcga cttgcccgcg cccgtctctc ccgtcacgac ggtgaggccg ggaccgagct
     6961 cgagatcgag gtcttcgacg atcgcgaggt tccggacggt caagcggcgc agcacacgcc
     7021 gattatgact gggtgtgtct cttcttctgc ccgatcggtc gcgccggaat tccgccagcg
     7081 atgacgccgt ccgcaaccgg cttggacgcc acccccatgg agccgaccag agcgtcggct
     7141 ccgatctcga cccccgagaa gatgacggcg tggtaggtga tccgcgcgcg atcgccgatc
     7201 accgttcgac cgaggctgac gtcgtggatg tcctccaccg cgtgggagtg ggagaagatg
     7261 ttgacgtagt cggagatcga ggcgtcatcg ccgatcacca cctcgccgcg gtcgtcgatg
     7321 agaacgttgc gatgaatggt cacgccgttc ccgaccgaca ggttgtagcc gaacgagaac
     7381 tcgacaccgt ggaagatccg gacgtccctt ccacaccgcc ggaagacgcg cggggcgagc
     7441 atggcccgca tctgggtccc gagccgcacg ttccggccga ccggcgaatg gtcgaacatc
     7501 tgccagaacc agaggagcgg cttgacgggg gcccacttcg cgagatcgat ctcccggtag
     7561 tactccgctt ccagggttgc gtttcgtgga tcgaatgtcg cgagcagcgc ccgaatcgcg
     7621 agcggagagc ggttatcccc gatcgcctgc tcccaggaga cctcccgacc gagatagaga
     7681 tgggtgagcg cctcccgcac gacgacgttg cggtcgagcc tgctgtcatc gagcttcccg
     7741 gcgagctctt ccacgaagcg cggatagagt tcctcaccga gggcgccgac cgggaaactg
     7801 cggaggggca ttcgccgggg acggtagcag agcgtttccg ggcagtcaaa ctcgcggtcg
     7861 caggcgctca cgaggggctc gggcggctcc gcctccgaac catttcctca ggaactccat
     7921 cgtcagcgcg accccgaatc cggtcgctcc agccgcgtac ccggcatgtt ccttctcgaa
     7981 gtgcgcgacc ccggccaagt ccagatgcgc ccagggcact cccgcgggga cgaactcctc
     8041 caggaacact gccgcgttga tcgtgccgcc cggccggcca ccggtgttct tcatgtcggc
     8101 ccactccgag cgaaggttct ctcgatagtc ctcccacagc ggaaggcgcc acaggcgctc
     8161 cccggtggtc tcacccgctg agagaagact cgtggccagc ctgtcgtccc cggtgaagag
     8221 tgccgcgcac tcgtgcccga gcgcgacgat cacggcgccc gtgagcgtgg catagtcgag
     8281 cagaacgtcc gggcgcagct tcgccgagta cgtgagtgcg tcggcgagga cgagccttcc
     8341 ctcggcgtcg gtattgtcga cctcgacgct cttcccgtta cgcatccgga gaatgtcgcc
     8401 gggtctcgtc gccgaaccgc tcggcaggtt ctccgtcaac ggcgtgagcg cgaccacgcg
     8461 gaccggaagt cggagcgact ttgccgcgcg gacgcaggcg agcgcggtcg ccgcgcccat
     8521 catgtcgtac ttcatctcgc ccatggacgg cgcgggcttg atcgagatcc cgccggaatc
     8581 gaacgtgatc ccttttccga cgagcaccgc ggtgccgcgg ggattccgcg gccgatagtc
     8641 ggcgacgacg aacgccggcg ggcgcacgct cccggcgttc accgcgagga gacccgccat
     8701 tttctcggcg gcgatcgcgc gcctcccgag aacccgcacc gcgaagccgt gccgcttgcc
     8761 cgcggcgacc gcttcccgcg cgaggtcggc ggggccgagg tcgttggccg gcgtgttccc
     8821 gagatcgcgc gcccaggaga ccgcttcccc gacgacctgc gcctcgcgcg ccgccgccga
     8881 gagctcggcc acgtccacgc cctcgggcgg gacgacgact gcggtcgtct ctccgggcgg
     8941 ccgcactttc ttgcgcgccg acttgtaacg ctcgaacgcg tagtcgccga gggcgagatg
     9001 cggcagaagc gcccgcaacg tgctcccgtc gatacccgca ccgaaggcga gcaggagacg
     9061 cgagcgcgac cgcgacgcgt tcacgccgag cgcgcggcgc agcgcccgac ggagcctcgc
     9121 gagcgacgcg ggcgcgcggc cgaggccgac gagaagccac gatccgtcgt cgcccgccgc
     9181 ccccgccgtc tcgcgatccc ggccgcgaaa actgctcgcc cgcgccgctt tccggatcga
     9241 gccggcgaga gcgggtggga agccgcggcc cgcatcccgt ccctcgtgga cgaatgcgac
     9301 ccgccagaag gcggcggaac gggagagcgt ggaaacgatt ttcaagcgca tgaaatcacg
     9361 ataacagacg cgtgccgcgc ccggtgtctg tctcgcgttc ccgtgctagt gttgccgcgc
     9421 gatggcgctc gcaacagtca ccgaaacgca tcttgccggc gcccggctct cacgtcgcgg
     9481 aaaagtccgc gagctctacg acctgggcga ttccctcctt ctcgtcgcga gcgaccgcat
     9541 ctcggcatac gactgcgtgc tctccccggg catccccgac aagggccgga tcctgaccca
     9601 gctgtccacg ttctggttcc gcaagttcga cgacgtcgag aaccacctca tcgaagcgga
     9661 cgtctcccgg tttcccgagc cgctgcaggc ggacgcgccc ctcctcgcgg ggcgatccgt
     9721 catcgtgaag aaggcggagg tgattccctt cgagtgcgtg gcccgcggat atctcgccgg
     9781 ctcgggctgg gcggagtacc gcaagacggg cgaagtctgc ggcgttcggc ttcctcccgg
     9841 cctctcggag gcggagcggc tcgcggagcc catcttcacg ccggcgacga agagcgctca
     9901 cggccacgac gaaaacgttc ccttctcgcg cgtcgtgaac gacctcggcg aggaagaggc
     9961 gatcttgatt cgcgacttga cgctctcgct ctacgaacgc gcgcgcgagt acgccgagtc
    10021 gagaggcctg ctgctcgcgg acacaaagct cgagttcgga cgactcgacg gtcgcctgct
    10081 ctggatcgat gaggcgttca ccccggactc ctcccgctac tgggacgcgc acgcctggac
    10141 gcccggacgt acgcccgtgt cgttcgacaa gcagttcgtg cgggactggc tcgactcgac
    10201 cggctgggat catcgtcctc ccgcgccccg cctgcccgag ggggtcgttc ggacgacccg
    10261 ggagaagtat ctcgaggcct atcggacgct caccggctca ctcccggcct acctctcctc
    10321 ctgaggcagc cgtctccgcc gggggacagg cgggcgttcc tggcacgaca tttgatggta
    10381 ggtccttgat gccctgactc tggggcttca acaaggaggg aaacatgccc aacccgctca
    10441 gactcatcac tctcgttctc gccgttggag cgtttgccgc cgtcgctcag gcgcagaacg
    10501 cggattacaa caccggtggt cccacggcga acactctcca gctcaaactg gtcgagcccg
    10561 cggaaggcgc gacgatcacg ggcaaccagg ttcgagtcgg tgtctcctac aacacgcaca
    10621 acttcggtga aggacagggc acgaagttcg gagaggccaa cttcccgcag ccgaccttcg
    10681 acatcttcct cgacaacgat ctccggaaga ccgtcaaggg caccgaagcc aacgtcgtgt
    10741 tcctcgacaa cgtcccggcg ggcgcccaca agatcgtcgt catggcgaag aacgtgagcg
    10801 gcgaagtgat cgaccgcaag caggtcaaca tcacgaccgt ggccgccgtc gcggaagctg
    10861 cccccgctcc ggccacgacc acgtatgagg cgcgcaccgc accgccaccg cctccggctc
    10921 ctgtcatcga agcacccgct ccgcctccgg ctcccccggc tccgatggag cgcgagacgc
    10981 tgccaaagac ggcctcctcg tatccgacgc tggcagtcgc cggattcgct ctcgtcgccg
    11041 tcgggctctc gctcggacgg aaggtctcct aggtattgtt ctcgccggcc ccgcctcggg
    11101 cggggccgag cgtttcttcg agcctgacga cggatccggt ccaccgacgg tgctcaagag
    11161 cctgtttcga cgcgtcttcg ccgccgccgg caccctgctg gtcgcggcga tcttactcat
    11221 tgtggcgggc tcgctcgaga tgttccggcc ttcgttctcc tactcgcaga cgccgccgtc
    11281 gccgacgctg gggcgcatct gcccgggcga cacgatcgct cggatgtcgg tctcccgatt
    11341 gaagtacgag gcaccggttc gtgaaggcgc cgatgccgac acgctcgcgc aggggcccgg
    11401 acacgttccg ggcacggctc tgccgggtga ggattccggc cgcaaacact ccgtcatcgc
    11461 gctcgcacgg gacgcgggcg caggattcgt ctcgcagctc gtcatcgggg acgtcatcga
    11521 gctgcggact ccgttcggcc ttcgcagtta tcgggtcgtc gcgcgcagca cggtcaaggc
    11581 ggaggacctc cgcatcgagc cgacggacga accgacgctg accctcgtcg cgccttaccc
    11641 cgccgactcc gtcggcccgg cgccactgcg gctggccatt cgcgcggaac cctcacctcc
    11701 cggtcttgcg ccggcgagct cggagatgag ctcggcgacg cgatcgaccg gggtgagcgc
    11761 aacgctcgcg gaggccgcag tctccgaggc atttccggaa ggcaggtaga tcctcccgaa
    11821 accgagtgcg gccgcctcgc ggactctgga cgccgccgcg ggcacgcgcc ggacttctcc
    11881 cagcaggccg atttcgccga aaaagaccgc cgaatccgga agcgcgtggc cccgcgcgga
    11941 ggaaatcagc gcggccacga tcgcgaggtc cgcgccgggt tcctcgatct ggaggcctcc
    12001 ggcgcacgag acgaagacct cccgcgaaga gagcgggatc ccggctcgct cgaggaccgc
    12061 gaggagcagc gacaggcggc cggaatcgac gccgatgacc acccggcgcg gagatcccgc
    12121 ggttgccggg ccgacgagcg cctggatctc gacgagcagc gatcgcgtcc cttcccgggt
    12181 cgccgtgacg gcgctcccgg gaagacccgg ccgcctttcg gcaagcagcg cggccgaggc
    12241 atttggcacc tctgcgagtc ccctcgccgt catttcgtag agcgcgactt cgtcgaccga
    12301 gccgaaccgg ttcttcgtcg cgcgcaggat tcgacgggag gagccacggt cgccgtcgac
    12361 cgcgatcacg gcgtcgacga ggtgctcgag cgaacgggga cccgcgaggc ttccatcctt
    12421 cgtgacgtgg ccgacgagga ggaccgggac cgcgcgctcc ttcgcaaagc gaacgagcgc
    12481 cgccgccgac tcgcgcacct gcgacaccgt gccggccgcc gacgagaggg cggcgttgcg
    12541 caccgcctgg atggagtcga cgacgacggc ggaccgctct ccagccgacg attccgcgac
    12601 gatcctctcg acgtccgtgt cggagaggac gagcagcttc tcgtgcccga cgccgagccg
    12661 atcggcgcgc agccgcacct ggtcgacgga ctcctccgcc gctgcgtaga gaacggctcg
    12721 ggagtcggta gcgagcgcgt gggcggcctg cagcaggagc gtcgacttgc cgatcccggg
    12781 ctcgccgcca acgagcacga ccgatccggc gaccagcccg ccgcccagca cgcggtcgaa
    12841 tcccggcaaa cccgtctcga ggcgtgctcc ggcggcggcc gaggcctccg aaagcgggcg
    12901 cgagcgcgga gggccggagg gggccgtcgt cggagcggcg cgggtctcct cgacgtaggt
    12961 gttccactcg gcgcagtcgg ggcagcggcc cagccacttc gacgaggtgg cgccgcagga
    13021 ttggcacgcg aagacggaag tgggcttagg tgacatctgc ctccgagaat cggaaatcgg
    13081 aaatcggaga tcgaaaagaa tccaagagag tcttgtcgcc agccgatttc ccggccaccg
    13141 attcccgatt accgtttcag gggtatcgac cccatcaggt acttgatgtc gtcatcgctc
    13201 cagcgcaggc cggcgccgat gaagagcgaa tcggcattgc ggcgccggtt gagaaaatcg
    13261 tcccacccgc cggtgacgaa gaccgacggc gagaagtagt agcggccggc gagcttcgcg
    13321 tgggccgaga agtcgcccgg ccggttgaaa tcccacacgt cggcggaaaa gcgcagccgg
    13381 tccttgagca tcatgtagtc gacgccggcg ccgccccgcc cctcgatcaa tcctccgcgc
    13441 gcgaccaggt cattcatccg aaacccgaag agcgccgaaa ttgcgaagtc gtccttgaac
    13501 tcgtcggttt cggtggtggt ctgctcggtg tgcccgtcgg ggaacgtcgt ccgctcgacc
    13561 gtcgtcgagc tcttgcgccg tccgaaaggc tgagtcgcga gctcgatgcg atagaagcgg
    13621 gcagtgtcgg cgggttggat gtcgagcgtg aagtaaccct ggccccggtt gtgcgccgcg
    13681 aggtgctccg cgcgcatccc gaggtcgatc ttcgtcttcc cgagcgcgcc gatcgccttg
    13741 ttgaaactgg ccaggccctc cttgacggcg accagggcgt cgttgatgtt cttgtgggtc
    13801 tcgtcgctct gaacgagctt gccgacggtc ccctccccct ccttgatccg gccggtgatc
    13861 tggttcaggt tgtcggcggt ggtctcgagt tttccggaga cctcgcggat gttcgacaac
    13921 ccctgcgtga cgttgccctg gttcgccgcc accacggagt cgacccgggc gacgagctcc
    13981 ttcatcgcgg tcgagaactc gcggaagttc gccaccgtcg cgtccacccc cgcgcggttc
    14041 gactcgacca tcccgcggat ttgctcgctg atcgcgcgca tgttctcgac gatgttctcg
    14101 agtcgctcct cgccccccgg acccccgatc gactgcttca ggctctgcgt gatctccttg
    14161 acgtccactc cgatgtcccg cgcgagcttc gtgatctcgt cgaaggacac gggcatcccg
    14221 ccgacgatca cggcgttgtc cggcagcggc ggagcgccga agggtccagg gacgagctcg
    14281 acatatttct ctcccagcag accgaggctc tcgatcgagg ccgaggcgcc ctggcgcagc
    14341 gcgacgtcgt ggtcgagctc gacgtcgacg agcgccctgc ccgcccccgt caggcggatc
    14401 ttgctgacgc ggccgacgcg aacgccggcc acgcggaccg cggtcttctc gtccagaccg
    14461 gcgacgttct tgaactgcac cgtgactttg cggggcgcgc cgccccgtcc aatccgcagg
    14521 tcctcgatct tccagatgaa gagcgcgatg attgcgagaa caaccaggaa aaaagcgccg
    14581 accttcgccg ccgtattcat gcgccctcca aacgcgccga ctcgctcttc cggtcgaact
    14641 ctaccgtgag cggcccttcc gcgcgtccct cgacgaactg ccggacagcc gggtcgtcgg
    14701 aacgcagaaa ttcttcgggg gaggcctggt agagcagtcg ccccccgtgg atgagcgcca
    14761 cccgatcccc gatcttgcgc gcgctctcca tgtgatgcgt gatcgtcacg atcgtgacgt
    14821 cgagtcgctc cttgagctcc aggatgacct cgtcgat
//
LOCUS       DASWAV010000239         2052 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U2665
            k141_14019, whole genome shotgun sequence.
ACCESSION   DASWAV010000239 DASWAV010000000
VERSION     DASWAV010000239.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960316
            Sequence Read Archive: SRR10260007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2052)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2052)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:47:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,929
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2052
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_14019"
                     /isolate="SMAG_U2665"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.36444 N 132.31889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..579
                     /locus_tag="VF958_04910"
     CDS             <1..579
                     /locus_tag="VF958_04910"
                     /inference="COORDINATES: protein motif:HMM:NF024674.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00282 family metallophosphoesterase"
                     /protein_id="HEX9148485.1"
                     /translation="IWDKKEIYEVLNSSDRLLRPANYPPGNPGRGVTVQQTAAGVPVA
                     VLNLQGQVFMPAQCDSPFRVADEELARLSDVKVIVVDMHGEATSEKVAIGWYLDGRVS
                     AVLGTHTHVATADETLRPGGTAYQTDVGMTGAYEGVIGFARERVIEKFLAQTPKAFET
                     AKRDVRLSGALVEVDPETGRALAIERIQVKVE"
     gene            623..1450
                     /locus_tag="VF958_04915"
     CDS             623..1450
                     /locus_tag="VF958_04915"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020537416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEX9148486.1"
                     /translation="MTSFNEEVNIRECLESIAFADEVLLVDSFSTDHTLEIAREIPGV
                     RILQREYFGSAAQKNWAMDQTLNPWILIVDADERVTPALAREILDLLERGPAADYYSI
                     RRRNLFLDRLIRHSGWSTDRVVRLVRRGAARYPKRRVHADIRPEGHAPVLEHALLHYT
                     FRSLGQYLEKLHRYAEWGAADAYRKGKRPGAVQLLLRPLWRFVRMYVFQAGFLDGLHG
                     LVLCTLQAYGVFLKWAKLWEWRRFDENGWAFDLPAYDDSEETWYGAPGEEARPAHVR"
     gene            1440..1919
                     /locus_tag="VF958_04920"
     CDS             1440..1919
                     /locus_tag="VF958_04920"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: protein motif:HMM:NF012787.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_except=(pos:1596..1598,aa:Sec)
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HEX9148487.1"
                     /translation="MFGKRPASLPLAPGDKAPEFALPDHTGAIRRLSEFRGRNVVLWF
                     YPKARTPGUTTEGRGFRDLAGEFEKKNAQIIGISFDPVKSNAAFVAKERFPFPLLSDE
                     RREAGLAYGACDSPEARSARRISYVIDEKGRIAAAYPAVSPADHPREVIDFLASRRA"
BASE COUNT          342 a          689 c          683 g          338 t
ORIGIN      
        1 atctgggaca agaaggagat ctacgaggtt ctgaactctt ccgatcggct gctgcggccg
       61 gcgaactatc cgccgggcaa cccggggcgc ggcgtgaccg ttcagcagac cgcggccggc
      121 gttccagtcg cggtgctgaa cctccagggc caggtcttca tgccggcgca gtgcgattcg
      181 ccctttcgcg tcgctgacga ggagctcgcg cgtctctccg acgtcaaagt gatcgtggtc
      241 gacatgcacg gcgaggccac ttccgagaag gtcgccatcg gttggtatct cgacgggcgc
      301 gtttcggcgg tcctgggaac gcacacccac gtcgcgaccg ccgacgagac cctccgtccc
      361 gggggcaccg cgtaccagac cgacgtcgga atgacgggcg cttacgaggg cgtcatcggt
      421 ttcgcgcgcg agcgcgtcat cgagaagttt ctcgcgcaga cgccaaaagc cttcgagacc
      481 gcgaagcggg acgtccgcct gagcggagcg ctcgtggaag tcgatccgga aaccggacgc
      541 gccctcgcaa tcgagcgaat ccaggtcaag gtcgaatgag ggcgccggag cagtgatgtc
      601 gagagagaag ctctcagcaa tcgtgacgtc attcaatgaa gaggtgaaca tccgggagtg
      661 cctcgagagc atcgccttcg ccgacgaggt gttgctcgtc gactccttct cgaccgacca
      721 cacgctcgag atcgcccgag agataccggg agtccgcatt ctccaacgcg agtacttcgg
      781 ctccgctgcc cagaagaact gggcgatgga tcagacgctc aatccctgga tcctgatcgt
      841 cgacgcagac gagcgcgtga cgccggcgct cgcgcgcgaa atcctcgacc tgctcgagcg
      901 aggtcccgcc gccgactact actcgatccg gcggcgcaac ctcttcctcg accgtctcat
      961 ccggcactcg ggctggtcaa ccgacagggt cgtccgactc gtccgccgcg gagccgctcg
     1021 ttacccgaaa cggcgcgtgc atgccgacat ccggccggaa gggcacgcac ccgttctcga
     1081 gcacgcgctc ctccactaca cgttccgctc gctcggccag tacctggaga agctccaccg
     1141 ttatgcggag tggggcgccg cggacgcgta ccggaagggg aagcgcccgg gcgccgtcca
     1201 gctcctgctg cggccgctct ggcggttcgt ccgcatgtac gtctttcagg cgggtttcct
     1261 cgacgggctc cacgggctcg tcctttgtac gcttcaggcg tacggggtct tcctcaagtg
     1321 ggcgaagctc tgggagtggc gccgcttcga tgagaacgga tgggcgttcg acctgcccgc
     1381 gtatgacgac tcggaggaga cgtggtacgg tgcgcccgga gaggaggctc ggccagccca
     1441 tgttcggtaa gcgccccgcc tccctgccgc tcgcgcccgg ggacaaagcg cccgagttcg
     1501 cgctgcccga ccacacggga gcgatccgcc ggctgtcgga gttccgaggc cgcaatgtcg
     1561 ttctctggtt ctacccgaag gcacggacgc ccgggtgaac gaccgagggg cgcgggttcc
     1621 gcgacctcgc cggcgagttc gagaagaaaa atgcgcagat catcggaatc tcgttcgatc
     1681 cggtgaagag caacgcggcg tttgtcgcga aggagcggtt tccgttcccg ctgctctccg
     1741 acgagcggcg ggaagcgggt cttgcctacg gggcgtgcga ttcgcccgag gcgcgctcgg
     1801 cgcgtcggat ctcgtacgtc atcgacgaga aggggaggat cgccgcggcc tatcccgcgg
     1861 tgtcgccggc ggaccacccc cgagaggtca tcgactttct cgcgagcagg cgcgcgtaga
     1921 actccttcgc aggcccagcg aacgactctc ctccgaattc tcgcgcgcgt gcgcgacccc
     1981 gctctcccat ctgcgcccgc agcccggggt cgcgtcgcag tcgctcgagc gcggacgcga
     2041 ggtcggcagg gc
//
LOCUS       DASWAV010000240         6231 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U2665
            k141_331876, whole genome shotgun sequence.
ACCESSION   DASWAV010000240 DASWAV010000000
VERSION     DASWAV010000240.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960316
            Sequence Read Archive: SRR10260007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 6231)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6231)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:47:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,929
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6231
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_331876"
                     /isolate="SMAG_U2665"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.36444 N 132.31889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..187
                     /locus_tag="VF958_04925"
     CDS             <1..187
                     /locus_tag="VF958_04925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9148488.1"
                     /translation="LGLSVMRLREKDVWDRITKEMKVSAEDFQARITALGKTLGPPWR
                     ADEKLATAVGCLALATR"
     gene            complement(208..3591)
                     /gene="mfd"
                     /locus_tag="VF958_04930"
     CDS             complement(208..3591)
                     /gene="mfd"
                     /locus_tag="VF958_04930"
                     /inference="COORDINATES: protein motif:HMM:TIGR00580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003690 - double-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HEX9148489.1"
                     /translation="MPEAGDPNLLVEIARGIGEYRSLVERLHPPQSNEPGTTVAGATG
                     ALPGVLLAALAGDLGRPLAIVVADEEESERIRQDLAAAGLDRVFHAPAPALTPYQRIP
                     PSLKARRDEFALLSALAQPASVDAIVLPARALFTRLPASERLRELSFALQEGQEITLT
                     RLVSQLTRVGYRRADLVIETGDLAVRGGLFDVFPPDRDLPLRVELDGDRIASMRVFDP
                     DTQRSRERVASALIPPFASGVDALEPRTLMEQRIGRPPSEAERIVFAPAVEAMPAGWL
                     DHARDAVLVVVEPSAVGEELDAFAERVEADRDPDRDFFAPEELLHPVERVRDAIAKAS
                     VFFDRVGLSARGALLRLPAEPIAAHGGRTAEAAGEISRAIAAEEEIFVAVRPTGGAEK
                     LRRFALEYSLHVSSQRRPGALACIPAEISAGFRLREPRLAVYSESEIFGEEKRSAVAR
                     GRPSEAFLSDLRDLKLGDAVVHRDYGIGLFQGLTRVPVEGEEREFMEIGYAEEKRLLL
                     PVERLDLVQKYSGVEGVQPALDKLGGAGWARRKASVKKAMKDMTDQLLKLYARRSLAE
                     GFAFSRDAPWQKEFEDAFEYEETPDQAQAIADVKRDMQQPRPMDRLLCGDVGYGKTEV
                     AMRAAFKAVLDGKQVAVLAPTTILADQHFRTFQRRFASFPVTIELLSRFRSRAEQKEI
                     VEKVAAGSIDILIATHRMLGKDLAFRDLGLLIVDEEQRFGVAQKERLKEWKASIDVLS
                     MSATPIPRSLHLGLSGLRDLSIIETPPRDRLAIETQVVPSKPEVIREAIEAEIERGGQ
                     VFFVHNRVETIGRVKQMLEDLLPRVRVAVAHGAMREAELEKAMMRFYSRDADVLLATT
                     IIENGLDIPTANTMLIDRAETYGLAQLYQLRGRVGRSDKPAYAYLLVPENLALSETAR
                     RRLASIQEFCDLGAGFRIAAKDLEIRGSGNILGGEQSGHIASVGFEMYLSMLEETVRE
                     IKGEEVRPERAVTLSLGLDLTIPPAYVADENWRMMIYKKVARARDDRELEEVRKEIGD
                     RFGEPPAALGRLIEYSRLRNRAERIGVTTVTRQAGRVHLRLAEDARVDGERLAALVRR
                     TSGATLTPGGVLSFPAPVADELLPVILSWMSELERREAA"
     gene            complement(3591..4202)
                     /locus_tag="VF958_04935"
     CDS             complement(3591..4202)
                     /locus_tag="VF958_04935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9148490.1"
                     /translation="MTGTVPATATGCAACGAARVFVVCPECGVIFDAPSDAFGMFGFA
                     RSWAVDVTLLEQRYELISRVLREKSSAREDAREKVPDAIEALQSARRLLLDPFERARY
                     LLTAYGGSEREKPARKREFQSEMMEIDAAAAKARRDGDAARLAAIVLEAEEKIASSIM
                     AAGQSFTRLERSRVEELGAAADALAEARIWRRKVDELRAPLGG"
     gene            complement(4199..5428)
                     /locus_tag="VF958_04940"
     CDS             complement(4199..5428)
                     /locus_tag="VF958_04940"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011984956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0044571 - [2Fe-2S] cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IscS subfamily cysteine desulfurase"
                     /protein_id="HEX9148491.1"
                     /translation="MKLPIYMDHHATTPVDPRVLEAMLPYFTRAFGNAASRSHVFGWE
                     AEAAVDEARNQVAVGMNADPREIVFTSGATESDNLAILGSAHAYGEKGKHVITGATEH
                     RAVLDSCEVLQREGFEVTYLAPDRTGSIDAGQVREAMRDDTILVTLMLANNEIGTIHP
                     IADIGAVCRERGVLFHTDAVQGFGKIPFDVGAMNVDLASVTAHKLYGPKGIGALYVRA
                     RRPRVRVAPILHGGGHERGLRSGTLNVPGIVGFGKAVEISIAVREEEARRLRMLRRRL
                     FDGILRDVSDVRLNGPDLPEITADGSLGPEAPERRLPGNLNLAFAGVEGEALLMGLKD
                     IAVSSGSACTSATLRPSHVLKAIGVPDDLAHASIRFGLGRESTAEEVDYAIGAVAATV
                     KRLRELMPGAGERRAAL"
     gene            5450..>6231
                     /locus_tag="VF958_04945"
     CDS             5450..>6231
                     /locus_tag="VF958_04945"
                     /EC_number="2.1.1.148"
                     /inference="COORDINATES: protein motif:HMM:NF014560.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050797 - thymidylate synthase (FAD)
                     activity [Evidence IEA];
                     GO_process: GO:0006231 - dTMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent thymidylate synthase"
                     /protein_id="HEX9148492.1"
                     /translation="MDRFAPTFQPVATGADAPNAGHPTRSPDAIMASPMSALPQTASN
                     FSFSSPRPSVRLVHAFERPFENVVATARTCYSSKGIVTEDQASAKPERRDALARSIYE
                     AGHHTTFQHAHFQFAIENVSRHFLWSFLHSHPFYNSEQVSQRYVEVKAGNYAVPPMLP
                     AERELFEATARKQEAAYRRLTELLVPIAGERYFGLFPGRGRDARGEKWSGAIQKRAQE
                     IARYVLPVATFAYLYHTVSGVTLFRYWRLCETYDAPVEQREVV"
BASE COUNT          976 a         2198 c         2068 g          989 t
ORIGIN      
        1 gctcggcctc tccgtcatgc gtcttcgcga gaaggacgtc tgggaccgga ttacgaagga
       61 gatgaaggtt tccgccgagg acttccaagc gcgcatcact gcgctcggaa agaccctggg
      121 acctccctgg cgagcggacg agaagctcgc gaccgcggtc ggctgcctgg cgctcgcgac
      181 ccgttagctc ttactctcag cttcccgcta agcggcctct cgtcgctcga gctccgacat
      241 ccaggaaagg atgaccggca gaagttcgtc ggcgaccgga gcggggaacg agagcacgcc
      301 tcccggcgtg agcgtcgcac ccgaagtccg gcgcacgagg gccgccagcc gctcgccgtc
      361 gacccgcgcg tcctcggcca gccgcaggtg tacgcggccg gcctggcggg tgacggtcgt
      421 gacaccgatg cgctcggcgc ggttgcgcag gcgcgagtac tcgatcaacc gcccgagcgc
      481 cgcggggggc tcgccgaacc ggtcgccgat ctccttgcgg acttcctcga gctcgcggtc
      541 gtctctggcg cgagcgacct tcttgtagat catcatccgc cagttctcgt cggcgacgta
      601 cgcaggcggg atcgtgaggt cgagcccgag cgacagcgtg acggctcgct ccggacgcac
      661 ctcctcgccc ttgatctcgc ggacggtctc ttcgagcatc gagaggtaca tctcgaatcc
      721 caccgacgcg atgtgacccg actgctcgcc gccgaggatg ttgcccgagc cgcgaatctc
      781 cagatccttc gccgcgatgc ggaacccggc gccgaggtcg cagaactcct ggatcgaggc
      841 gagccggcgg cgcgccgtct cggagagcgc gagattctcc ggaacgagga ggtacgcata
      901 cgcgggcttg tcgcttcgcc cgacgcgccc gcgcagctga tagagctggg cgaggccata
      961 ggtctcggcg cgatcgatca gcatcgtgtt cgccgtcggg atgtcgaggc cgttctcgat
     1021 gatcgtcgtt gccagcagga cgtcggcgtc ccgcgagtag aaccgcatca tcgccttctc
     1081 gagctccgcc tcgcgcatcg cgccgtgcgc cactgcaacc cgcaccctcg ggagcagatc
     1141 ctcgagcatc tgcttgaccc gcccgatcgt ctcgacgcgg ttgtgaacga agaagacctg
     1201 gccgcctcgc tcaatctccg cctcgatcgc ttcccggatg acttcgggtt tcgaggggac
     1261 gacctgcgtc tcgatcgcca ggcgatcgcg cggaggagtc tcgatgatcg aaagatcacg
     1321 cagtccggac aggccgaggt ggagcgagcg cgggatcgga gtcgcggaca tcgagagcac
     1381 gtcgatcgac gccttccact ccttcagccg ctccttctgg gcgacgccga accgctgctc
     1441 ctcgtcgacg atcaggaggc cgaggtcccg gaacgcgagg tccttcccga gcatgcggtg
     1501 cgtcgcgatc aggatgtcga tggacccggc cgcgaccttc tcgacgatct ccttctgttc
     1561 ggcccgcgac cggaagcgcg aaaggagctc gatcgtcacg ggaaacgacg cgaagcgccg
     1621 ctgaaacgtc cgaaagtgct ggtcggcgag gatggtcgtc ggcgccagta ccgcgacctg
     1681 cttcccgtcg agaaccgcct tgaaggccgc gcgcatcgcg acctccgtct tgccgtagcc
     1741 gacgtcgccg cagagcaacc ggtccatcgg ccgcggctgc tgcatgtccc tcttgacgtc
     1801 ggcgatcgcc tgagcctggt ccggcgtctc ctcgtattcg aaggcgtcct caaactcctt
     1861 ctgccagggc gcgtcccgcg agaacgcgaa tccctccgcg agactgcgtc gggcatagag
     1921 cttcaggagc tggtcggtca tgtccttcat cgccttcttg accgacgcct tgcgccgcgc
     1981 ccagcccgcg ccgccgagct tgtcgagcgc gggctgcacc ccttcgacgc ccgagtactt
     2041 ctgaaccagg tcgaggcgct cgaccggaag gaggagcctc ttctcctcgg cgtagccgat
     2101 ctccatgaac tcgcgctctt cgccttcgac cggcacgcgc gtcagtccct ggaagagtcc
     2161 gatgccgtag tcgcggtgga cgacggcgtc gccgagcttc aagtcccgca agtccgagag
     2221 gaatgcttcg gacggacgcc cgcgcgcgac tgccgaacgc ttctcctcgc cgaagatctc
     2281 gctctcggaa tagacggcga gacgcggctc gcgcagccgg aatccggccg agatctccgc
     2341 aggaatgcag gcgagcgctc ctggccgtcg ctgcgaagag acgtgcaacg agtactcgag
     2401 cgcgaagcgg cggagctttt ccgcgccgcc ggtcggacgg acggcgacga agatctcctc
     2461 ctcggccgcg atcgcccgcg agatctctcc cgctgcctcc gcggtgcgcc ctccgtgcgc
     2521 cgcaatcggc tccgccggga gacggagaag ggcgccccgc gccgagaggc cgacacggtc
     2581 gaagaagacg ctcgctttcg cgatcgcgtc gcggaccctt tcgacgggat ggagcagctc
     2641 ctccggagcg aaaaagtcgc ggtcggggtc gcgatccgcc tcgacgcgtt ccgcaaaggc
     2701 gtccagctcc tccccgaccg cggagggctc gaccaccacg aggacggcgt cgcgggcgtg
     2761 atcgagccac ccggccggca tcgcctcgac cgcgggggcg aacacgatcc gctcggcctc
     2821 cgacgggggt cgcccgattc tctgctccat gagcgtccgc ggctcgagcg cgtccacgcc
     2881 ggaggcgaac ggcgggatca gcgcggaggc gaccctctcg cgcgaccgct gcgtgtccgg
     2941 gtcgaagacc cgcatcgaag cgatgcggtc gccgtcgagc tcgacgcgca gcgggagatc
     3001 gcggtccggc gggaacacgt cgaagaggcc gccgcgcacg gcgaggtcgc cggtctcgat
     3061 gacgagatcc gctcggcgat atccgacgcg ggtgagttgc gagacgagcc tcgtgagcgt
     3121 gatctcctgc ccctcctgga gcgcgaagga aagctcccgg aggcgctcgg acgcgggcaa
     3181 acgcgtgaag agcgcgcgcg cgggcaacac gatcgcgtcg acgctcgccg gttgcgcgag
     3241 cgcggagagg agcgcgaact cgtccctccg cgctttcagc gacggtggaa tgcgctggta
     3301 tggcgtgagc gccggggcgg gggcgtgaaa gacccggtcg agcccggccg cggcgaggtc
     3361 ctggcgaatc cgctcggatt cttcctcgtc ggccacgacg atggcgaggg gccgcccgag
     3421 gtcgccggca agcgccgcga gcaggacgcc gggaagggcc ccggttgctc ccgccaccgt
     3481 ggtgccgggc tcgttggact gcggcgggtg caggcgctcg acgagggagc ggtactcgcc
     3541 gatgccgcgc gcgatctcga cgagaaggtt cgggtctccc gcctccggca tcaaccgccg
     3601 agcggggccc ggagctcgtc cacttttcga cgccagattc gcgcctcggc gagcgcgtcc
     3661 gcggcggcgc cgagctcctc gacccgggaa cgctcgagcc tcgtgaagct ctggccggcc
     3721 gccatgatcg aggaggcgat tttctcctcg gcttcgagca cgatggcggc gagccgcgcc
     3781 gcgtccccat cccggcgtgc tttcgccgct gccgcgtcga tttccatcat ttcgctctgg
     3841 aactcgcgct tgcgagcggg tttctcgcgc tccgagccgc cgtacgccgt caggaggtat
     3901 cgcgctcgct cgaaggggtc gagcagcagc cgcctcgcgc tctggagggc ttcgatcgcg
     3961 tcggggacct tctcacgcgc gtcctcgcgt gccgaagact tctcgcgcag cacccgcgag
     4021 atgagctcgt agcgctgctc gagcagtgtc acgtccacgg cccacgagcg cgcgaacccg
     4081 aacatcccga acgcgtcgga cggagcgtcg aagatcactc cgcattccgg acacacgacg
     4141 aacactcgcg ctgcaccgca ggccgcgcac ccggtggccg tcgccggaac cgttccggtc
     4201 acaacgccgc ccttcgctcc cccgcgcccg gcatcagctc tcgcagacgc ttgaccgtcg
     4261 ccgcgaccgc gccgatcgcg tagtccacct cctccgccgt gctctctcgc ccgaggccga
     4321 agcggatgga ggcgtgggcg aggtcgtcgg ggactccgat cgctttcagg acgtgcgacg
     4381 ggcgcagcgt ggcggacgtg cacgcggagc ccgaagagac cgcgatgtcc ttcaagccca
     4441 tgagaagagc ttccccctcg acgccggcaa acgcgaggtt gaggttgccg ggcaatcgcc
     4501 gctcgggagc ttccggcccg agcgagccat cggcggtgat ctccggcagg tcggggccgt
     4561 tcaggcgtac gtcggagacg tcgcgcagga tgccgtcgaa aaggcgccgg cgcagcatcc
     4621 gaaggcgcct ggcctcctct tcgcggaccg cgatcgagat ctcgacggcc tttccgaagc
     4681 ccacgatccc cgggacgttc agggtccccg accgcaatcc ccgctcgtgg cctccgccgt
     4741 ggaggatcgg tgcgactcgc acccgcggcc ggcgcgcacg gacgtagagg gcgccgatcc
     4801 ccttgggtcc gtacagcttg tgagcggtga ccgacgcgag gtccacgttc atcgcgccga
     4861 cgtcgaaggg gatcttcccg aagccctgaa cggcgtccgt gtgaaagaga acccccctct
     4921 cccggcacac cgcgccgata tccgcgatcg gatggatcgt tccgatctcg ttgttggcga
     4981 gcatcaacgt gacgaggatc gtgtcgtcgc gcatcgcctc ccggacctgc ccggcgtcga
     5041 tcgagcccgt tcgatcgggt gcgagatagg tcacctcgaa gccctcgcgc tgcaggacct
     5101 cgcagctgtc gagaacggcg cgatgttcgg tcgcgccggt gatgacgtgt ttccccttct
     5161 cgccgtaagc gtgggcggag ccgaggatgg cgaggttgtc gctctccgtc gccccgctcg
     5221 tgaagacgat ttcccgaggg tccgcgttca tcccgacggc gacctggttg cgcgcctcgt
     5281 cgaccgccgc ctccgcctcc cacccgaaga cgtggctgcg cgacgcggcg ttcccgaacg
     5341 cgcgggtgaa atacgggagc atcgcctcga gcacccgcgg atcgacgggc gtcgtggcgt
     5401 ggtgatccat gtagatgggg agtttcaccg acatatcgaa actctcaata tggaccgttt
     5461 cgcccccact tttcaaccgg tcgcgaccgg agcggacgcg ccgaacgcgg gacacccgac
     5521 acgatctccc gatgccataa tggcctcgcc gatgtcggct cttccccaga cagcaagcaa
     5581 cttttccttc agctctccga ggccctcggt ccgcctcgtc cacgccttcg aacggccctt
     5641 cgagaacgtc gtcgcgacgg cccgcacgtg ctactcctcg aagggaatcg tcaccgagga
     5701 ccaggcgtcg gcgaagccgg agcggcgcga cgcgctcgcc agatccatct acgaggcggg
     5761 acaccacacg accttccagc acgcgcactt ccagttcgcc atcgagaacg tctcacgtca
     5821 cttcctctgg tcgttcctcc actcgcaccc gttctacaac tccgagcagg tctcccagcg
     5881 ctacgtcgag gtgaaggccg gcaactacgc ggtgccgccg atgctgccgg ccgagcgtga
     5941 gctcttcgag gcgaccgcac ggaagcagga agccgcgtat cgccggctga cggagcttct
     6001 cgtcccgatc gccggcgagc gatatttcgg gctctttccg gggcgcggcc gggatgcccg
     6061 aggggagaaa tggtccggcg cgatccagaa gcgcgcccag gagatcgcgc gttatgtcct
     6121 gcccgtcgcg actttcgcgt acctctacca cacggtctcc ggcgtcacgc tcttccgcta
     6181 ctggcggctt tgcgagacgt acgacgcgcc ggtcgagcag cgagaggtcg t
//
LOCUS       DASWAV010000241         2887 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U2665
            k141_613150, whole genome shotgun sequence.
ACCESSION   DASWAV010000241 DASWAV010000000
VERSION     DASWAV010000241.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960316
            Sequence Read Archive: SRR10260007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2887)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2887)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:47:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,929
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2887
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_613150"
                     /isolate="SMAG_U2665"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.36444 N 132.31889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..399
                     /locus_tag="VF958_04950"
     CDS             <1..399
                     /locus_tag="VF958_04950"
                     /inference="COORDINATES: protein motif:HMM:NF013798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macro domain-containing protein"
                     /protein_id="HEX9148493.1"
                     /translation="EECDRIGHCDVGQAVVTGAGNLPARWVIHAVGPVWRGGNEGEEI
                     QLASAVFSALKRAEEIGAKSVALPMISTGVFGFPIEIATRITVAAARAFAQKAGSVER
                     IVFCLPDEEKYGRVRDQLEEAETSGGDRST"
     gene            506..>2887
                     /locus_tag="VF958_04955"
     CDS             506..>2887
                     /locus_tag="VF958_04955"
                     /inference="COORDINATES: protein motif:HMM:NF025910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FG-GAP-like repeat-containing protein"
                     /protein_id="HEX9148494.1"
                     /translation="MLCAILILSLLPASPERNAVDLSRLETAKNVGLAALEEGNLEEA
                     RRRFETVRQLAPGEALGWANGAVVALRAKDLERAKELLGDALRRTPNDARVLAIEGTR
                     RELAGDSAGAADAFEKAASLDPSDLMSRWAAARLLSEKISGGGPRAIAALEAALERAP
                     SNLFLLARLSELLRRSGDAGRALQAHDRLARALEGADAKLDRYLGEARQALTAGDAAT
                     ASLKYRIVENLLKVSPRYQQSRQDVEPGVVGLPIEDWSPALAADVRARAGRLIRVSFA
                     AATPQALTALKGLTAVRATGASGRDLVFAGSSGVVVAERSGAGYRAQPVLVGSFDFVE
                     TADVTNSGRFDFIAPGALLIAGPAGWSRTDAPKADAVTPIDYDNDGDLDLYFSSSAGD
                     RLLRNNLDGTWTDVTRAAGLPASLASRSAIAGDFDRDGDPDLVVLQSGGGLLLLDNLR
                     GGRFAEKSAGLPREGAFRWGAAGDLDADGRLDLVWARADAAFVARNRGDGTFAPAKAL
                     PSAGKPVLADFDNDGFLDLLLASGGASTMLRGDGAGGFGRWGVGPLPAALDGEPVDFD
                     GDGDLDLALVTAGGEASLLENRGGNANGWMDVTLEGLTTGSAKVNRAGYGSELELKAQ
                     ELYVYRVANRPVTHLGLGARRKAEVLRIVWTNGIRQNELDPRTRVVVKEVQQLKGSCP
                     FLYAWDGKRWSFVTDVLGNSPLGLLYDGVHQAPADTREWLLVKGADLEPSDGRLSLEL
                     TEELWEAAYVDLAELSALDHPRGIEITPSSAAMTPPPFPEDRIFTISRPITPKA"
BASE COUNT          433 a          978 c         1013 g          463 t
ORIGIN      
        1 gaggagtgcg accggatcgg ccactgcgac gtcggtcagg cggtcgtgac cggcgccggt
       61 aacctgcccg ctcgctgggt gattcacgcg gtcggacccg tatggcgcgg gggcaacgag
      121 ggggaggaga tccagctcgc ctctgccgtg ttctccgcgt tgaagcgggc ggaggagatc
      181 ggggcgaaat cggtcgccct ccccatgatc tcgaccggcg tcttcgggtt tcccatcgag
      241 atcgccacgc gcattacggt cgcggctgcc cgcgcattcg cgcagaaagc tggcagcgtc
      301 gaaaggatcg tcttctgcct acccgacgag gagaagtacg gccgagttcg cgatcagctc
      361 gaggaggccg aaacgtcagg cggggatcgc tcgacttaat gctcggcgca acgcgccgga
      421 aagcgcgatc tctcgcgggc aatccctccg gcacctgcct tcactcaagt cttgtcgcgg
      481 gaagtgcttg gttatgatcc cgggcgtgct ttgcgcgatc ctgattctct ccctgttgcc
      541 ggcctctccc gagcgcaacg ccgtcgatct ttcccgcctc gagaccgcga agaacgtcgg
      601 attggccgcg ctcgaggaag gaaatctcga agaagcccgc cgccgtttcg agaccgttcg
      661 tcagctcgcg cccggcgagg cgctcggatg ggcgaacggc gccgtcgtcg cccttcgcgc
      721 gaaggatctc gagcgcgcga aggagctcct cggagatgcg cttcgccgca cgccgaatga
      781 cgcccgcgtt ctcgcgatcg aagggacccg gcgcgaactc gccggcgact ctgccggcgc
      841 cgcagacgcg ttcgagaaag ccgcctccct cgacccgtcg gatctcatgt cgcgctgggc
      901 ggcagcgcgc ctcctttcgg aaaagatctc cgggggcggc ccgcgcgcga tcgcggcgct
      961 ggaggccgcg ctcgagcggg cgccgtcgaa cctctttctc ctcgctcgtc tctccgaact
     1021 cctgcgaagg tcgggggatg ccggccgggc tttgcaggct catgaccggc tggcccgcgc
     1081 gctcgaaggc gccgacgcga agctcgacag gtatctcggc gaggcacgcc aggccctgac
     1141 ggcgggtgat gccgcgacgg cgtcgctcaa gtaccggatc gtcgagaacc ttctcaaggt
     1201 ctcgccccgg taccagcagt cccgtcaaga cgtcgagccc ggtgtggtcg gactcccgat
     1261 cgaggactgg tctccggctc tcgccgcgga cgtccgcgcg cgcgccggac gcctcatccg
     1321 ggtgtcgttc gcggcggcga ccccgcaggc gctcaccgct ctgaaggggt tgacggcggt
     1381 gcgcgccacg ggggcgagcg gccgcgacct cgtcttcgcg ggcagttcgg gtgtcgtcgt
     1441 cgccgaacgc tcgggcgccg gttatcgcgc gcagcccgtg ctcgtcggat cattcgactt
     1501 cgtggagacg gccgacgtga cgaactcggg ccggttcgat ttcatcgcgc cgggagcact
     1561 gttgatcgcg ggacccgcag gctggagccg gaccgatgcg ccgaaggccg acgcggtcac
     1621 gccgatcgat tacgacaacg acggagacct cgacctctac ttctcatcga gcgccggaga
     1681 ccgattgtta cggaacaacc tcgacggaac gtggacggac gtgacccggg ccgccggcct
     1741 gccggcgtcg cttgcctcgc gaagcgcgat cgcgggcgac ttcgatcgcg acggcgatcc
     1801 cgacctggtg gtcctccagt ccggaggcgg gcttctgctc ctcgacaacc tgcgcggcgg
     1861 gcgtttcgcg gagaagagcg cggggctccc ccgggagggc gccttccggt ggggggcggc
     1921 gggcgatctc gacgccgacg ggcggctcga cctcgtctgg gcgcgcgcgg atgcggcatt
     1981 cgtcgcgcgc aaccgcggag acgggacctt cgcgcccgcg aaggcgcttc cgtcggcagg
     2041 gaagccggtg ctcgccgact tcgacaacga cgggtttctc gacctgttgc tcgcttcggg
     2101 aggcgcttcg acgatgcttc gcggcgacgg ggccggcggt ttcggaaggt ggggggtcgg
     2161 ccccctcccg gcggcgctcg acggcgaacc ggtcgatttc gacggcgacg gagatctcga
     2221 cctcgcgctc gtgacggcgg gtggagaggc ctcgcttctc gagaaccggg gcggcaatgc
     2281 caacggctgg atggacgtca cgctcgaagg gctgacgacg ggctcggcga aggtcaaccg
     2341 cgccggctac ggatcggagc tggagctcaa ggcgcaggag ctctacgtgt accgggtcgc
     2401 caatcggccg gtgacccatc tcggcctggg cgcgcgccgg aaggccgagg tgctgcgcat
     2461 cgtctggacc aacgggattc gtcaaaacga gctcgatcca cggacccgcg tcgtcgtgaa
     2521 agaggtccag cagctcaagg ggtcctgccc cttcctgtac gcctgggacg gcaagagatg
     2581 gagtttcgtg accgacgttc tcggcaacag cccgctcggc ctgctctacg acggcgttca
     2641 ccaggcgcca gcggacacgc gcgagtggct gctcgtcaaa ggggccgatc tcgagccttc
     2701 ggacgggcgg ctctctctcg agctcaccga agagctctgg gaagcggcgt acgtggacct
     2761 ggccgaactc tcggcgctcg atcacccgcg tgggatcgag atcaccccga gctccgcggc
     2821 gatgacgccc cctcctttcc ccgaggaccg gatcttcacg atctcgcgtc cgatcactcc
     2881 gaaagcg
//
LOCUS       DASWAV010000242         2894 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U2665
            k141_652279, whole genome shotgun sequence.
ACCESSION   DASWAV010000242 DASWAV010000000
VERSION     DASWAV010000242.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960316
            Sequence Read Archive: SRR10260007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2894)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2894)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:47:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,929
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2894
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_652279"
                     /isolate="SMAG_U2665"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.36444 N 132.31889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..910
                     /locus_tag="VF958_04960"
     CDS             <1..910
                     /locus_tag="VF958_04960"
                     /inference="COORDINATES: protein motif:HMM:NF036799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9148495.1"
                     /translation="ASGLHETYSYPFVDRRTDEASFAPWLESTGTAASPLSVANPVDA
                     SRRDLRATLLPGLLDAASRNFRHGSREVRLFEVGRAFGAPGDPSRPETFESRRLAFAL
                     GGEARTHWSVPAASRGTDFFDAKGLFERLVEPWLSDEELVWRPFAAPAFVAGAAARCE
                     SADGHLVGVVGVVGEAEREKRRLPEGVSAGEILIESIPREGRSVRFRVYSAFPPIVAD
                     LSFAQPRSIAWEAIEEFARGLALSDLESLKLLDRYEGPGVAEGQVKTTIRLAFRSPER
                     TLEQDAVNRERDQLAQALRDKFGVLI"
     gene            927..1241
                     /locus_tag="VF958_04965"
     CDS             927..1241
                     /locus_tag="VF958_04965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9148496.1"
                     /translation="MDTSRIFDTLDRRIEKLLSRLESLEQENEKLKADLAAARRAEKD
                     AADSRGAVEKLKADLAAARRAEKEGADSRDAVERLERDQEAVRERLEKLIATLEAAEK
                     KA"
     gene            1352..1627
                     /locus_tag="VF958_04970"
     CDS             1352..1627
                     /locus_tag="VF958_04970"
                     /inference="COORDINATES: protein motif:HMM:NF017016.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000917 - division septum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HEX9148497.1"
                     /translation="MTQVEIFGQTYNVRAEGDSTYIHDLARLVDSRMKEVAERTATVD
                     TTKIAILAALNITDDLYQAEKRRTGEPADFRVRAERLIRKLDDALGA"
     gene            1810..>2894
                     /locus_tag="VF958_04975"
     CDS             1810..>2894
                     /locus_tag="VF958_04975"
                     /inference="COORDINATES: protein motif:HMM:NF023495.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rnase Y domain-containing protein"
                     /protein_id="HEX9148498.1"
                     /translation="MNTSIAIPIAAAIFAVAFALTAYFLLRAKVRRERAEIRVEADRV
                     LADAKKSADGRLREASIEAKEKVLAAQVEFDKAIQVRRQEVASLEKRVAQKEENLDRK
                     LAQQEKREEEFARKEKALAESQKGVERAHEEAERLIAEQQKRLEQISGLTAADAKRDL
                     IRSIENEARVEASGLMRRIEEEAIEQATQKARHQIGMAIQRLASDTVVEATVSVVELP
                     SDDLKGRIIGREGRNIRALEAATGVDLIIDDTPEAIVISAFEPIRREVARLALEKLIV
                     DGRIHPARIEEIVEKVRSELWEKIRQEGESTALEFSIPDLHPELIKLLGRLKYRTSYG
                     QNVLQHSKEVAWLASSMAAELGAKMEIA"
BASE COUNT          546 a          927 c          999 g          422 t
ORIGIN      
        1 cgcctccggg ctgcacgaga cgtactcgta tccgttcgtc gaccggcgaa ccgacgaggc
       61 gtccttcgcg ccctggctcg aatcgaccgg caccgcggcg agcccgctgt cggtggcgaa
      121 cccggtcgac gcgtcgcggc gcgatctgcg agcaacgctc ctgccggggc tcctcgacgc
      181 cgcctcgcga aacttccgac acggctcccg ggaggtccgt ctcttcgagg tcggccgcgc
      241 gttcggcgcg ccgggagatc cctcccgtcc cgagacgttc gagtcacgcc gtctcgcgtt
      301 cgcgctcggg ggtgaggcgc gcacccactg gagcgtgccg gccgcctcgc gcgggacgga
      361 cttcttcgac gcgaaggggc ttttcgagcg actcgtcgag ccctggctgt ccgacgagga
      421 gctcgtctgg cggccattcg cggcacccgc gttcgttgcg ggagccgcag cccggtgcga
      481 atcggcggat gggcatctcg tcggggtcgt cggggtcgtc ggcgaggccg aacgcgagaa
      541 acgccggctc ccggagggag tgtcggcggg ggagatcctg atcgagtcga tcccgcgcga
      601 gggtcggtcc gtccgcttcc gtgtctattc ggcgttccct ccgatcgtgg ccgatctctc
      661 gttcgcgcag ccccgctcga tcgcctggga ggcgatcgag gaattcgcgc gcggtctcgc
      721 gctctcggac ctcgagtcgt tgaaactcct cgaccgttac gaaggcccgg gggtggcgga
      781 ggggcaggtc aagacgacga tccgcctggc cttccgatcg ccggaacgaa cgctcgaaca
      841 ggatgcggtg aatcgcgagc gggaccagct cgcgcaggct ctccgcgaca agtttggcgt
      901 cctcatttga gataggtttc gaattcatgg acacgagccg aatcttcgac accctcgatc
      961 gccggatcga gaagctgctt tcgcggctcg agagcctcga gcaggagaac gaaaagctca
     1021 aagcggatct cgcggctgcc cgccgcgccg agaaggacgc cgccgattcg cgcggcgccg
     1081 tcgagaagct gaaggccgat ctcgccgcag cgcgtcgggc cgaaaaagag ggcgcggatt
     1141 cgcgcgatgc cgtcgagcgg ctggagcggg accaggaggc tgtccgcgaa cggttggaga
     1201 agctgatcgc gacgctcgaa gcggccgaga agaaggcctg acagccggca gaccggccta
     1261 aaccgttgac tcggaatggt tcccgaatct acactcagcc attgaaagga gtctttccga
     1321 agacgcagca gtggccaacc ggggaggcag cgtgacgcag gtcgaaatct tcggccagac
     1381 ctacaacgtt cgggccgagg gcgactccac ctacatccac gacctcgccc gcctggtgga
     1441 ctcgcgaatg aaggaagtgg ccgagaggac agcgaccgtg gacacgacca agatcgccat
     1501 cctcgctgcc ctgaacatca cggatgatct ttaccaggcc gagaaacgtc gaaccggcga
     1561 gccggcggac ttccgggtcc gggcggagcg cctcattcga aaactggacg acgccctggg
     1621 cgcctgaagg cgaggcgtcg gcatccccga agtgattcgg ggaacacgag atgaaacgag
     1681 cggaacgttg aagcaaggta cggttttccc agtccagaac ccgaaccctc gtcggaagag
     1741 tcacttccgg gaaatcgtgt agccgcgccc cgacttcggc cgccctcgat atgagtgagg
     1801 gagccgaaaa tgaacacatc gatcgcgatc ccgatcgcgg cggcaatctt cgccgtcgcg
     1861 ttcgcgctga ccgcgtactt cctcctgcgg gcgaaagtcc ggcgggaacg agcggagatc
     1921 cgcgtcgagg ccgatcgggt cctcgccgac gcgaagaagt cggcggacgg ccgtcttcgc
     1981 gaggcctcga tcgaggcaaa ggaaaaggtc ctcgccgcgc aggtcgagtt cgacaaggcc
     2041 atccaggtcc ggcgccagga agtcgcctcc ctggagaagc gagtcgctca gaaggaggaa
     2101 aacctcgacc gcaagctcgc ccagcaggag aagcgggagg aggagttcgc gcgcaaggag
     2161 aaggcgctgg ccgagtccca gaagggcgta gaacgggcgc acgaggaggc ggagcgtctg
     2221 atcgcggagc agcagaagcg gctcgagcag atctcgggtc tgaccgccgc cgacgcgaag
     2281 cgggacctca tccgatcgat cgagaacgag gcgcgcgtcg aggcgtccgg cctcatgcgt
     2341 cgaatcgagg aagaggcgat cgagcaggcc acgcagaagg cccgccacca gatcgggatg
     2401 gcgattcagc ggctcgcctc cgacacggtc gtcgaagcga cggtgtcggt ggtcgagctc
     2461 ccctcggatg atctgaaggg ccgcatcatc gggcgcgagg ggcgcaacat ccgggcgctc
     2521 gaggccgcga ccggcgtgga cctcatcatc gacgacacgc ccgaggcgat cgtcatcagc
     2581 gcgttcgagc cgatccgtcg cgaggtcgcc cggctcgcgc tcgaaaagct gatcgtagac
     2641 ggacgcatcc acccggcccg tatcgaggag atcgtggaga aggtccgatc ggagctctgg
     2701 gaaaagatcc ggcaggaagg ggaatcgacc gcgctcgaat tctcgatccc cgacctccac
     2761 ccggagctca tcaagctgct cggccggctg aagtaccgga cctcctacgg ccagaacgtc
     2821 ctccagcact ccaaggaggt cgcctggctc gcttcgagca tggccgcgga gctgggggcg
     2881 aagatggaga tcgc
//
LOCUS       DASWAV010000243         4118 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U2665
            k141_493665, whole genome shotgun sequence.
ACCESSION   DASWAV010000243 DASWAV010000000
VERSION     DASWAV010000243.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960316
            Sequence Read Archive: SRR10260007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 4118)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4118)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:47:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,929
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4118
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_493665"
                     /isolate="SMAG_U2665"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.36444 N 132.31889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..460
                     /locus_tag="VF958_04980"
     CDS             <1..460
                     /locus_tag="VF958_04980"
                     /inference="COORDINATES: protein motif:HMM:NF021883.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HEX9148499.1"
                     /translation="LDDSANRRIAIPEAFLTADAILVLLENVFSGLVVHADVARRRLE
                     AEIPFLATENILMEAVRRGGDRQELHEKLRVFARAAAERPAGTGRATDLLDRIAADAD
                     FGLTRAELDQAARPERLTGRSSEQVETFVREELDPALAGVEAVASVPLKV"
     gene            457..1119
                     /locus_tag="VF958_04985"
     CDS             457..1119
                     /locus_tag="VF958_04985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009733659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septal ring lytic transglycosylase RlpA family
                     protein"
                     /protein_id="HEX9148500.1"
                     /translation="MTPRDAVRVALLVAVLGAGAACASHPPARSVSHGPPAPGWTEEG
                     VASWYGGRDGFEGKPTASGEIYDSSLLTAAHRELPLGTVVDVTNLDNERTVRVRINDR
                     GPFVFGRVIDLSRAAAGKIGLIGSGTGSVRVTVVTPGIPLQITSPTGRWAVQVGSFAD
                     QLRAERHAERLRGTGRAVFLEPYRGLTRVKVGPFDGRREAEHELQLLEDDGFEGIVVP
                     TD"
     gene            complement(1154..2080)
                     /locus_tag="VF958_04990"
     CDS             complement(1154..2080)
                     /locus_tag="VF958_04990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007669791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-formamidopyrimidine glycosylase family
                     protein"
                     /protein_id="HEX9148501.1"
                     /translation="MPELPDIAAYLVAIEARVAGKPLESVRLGSPFVLRSVDPPLPEV
                     EGRRVIGLRRLGKRIVFDLEGDLHIVLHLMIAGRLHWKTEAVPVAAKNSLAAFDFLDG
                     TLLLTEAGSKKRASLHVVRGEAALAEHARGGLEVLEADRDSFRAALTRENHTLKRTLT
                     DPRLFSGIGNAYSDEILHRARLSPAKLTSRLSAEEISRLHGATRDVLEDWTGRLTEEA
                     RKRFPEKVTAFREGMAVHGRFGKPCPVCGTKIQRIVYAENESNYCPTCQTGGRLLADR
                     ALSQLLKGDWPKTLEELEDRKRTPSPSGRGTG"
     gene            2106..>4118
                     /locus_tag="VF958_04995"
     CDS             2106..>4118
                     /locus_tag="VF958_04995"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding domain-containing protein"
                     /protein_id="HEX9148502.1"
                     /translation="MSLLSRYTHWLHTRWPAGTVEKLPDIREDGTTAVPGVRVVGDLT
                     GVPLLKFSSDSGARAVHAILREPEFLQTRPREPDVVDLAIVGGGVSGIAAAIAAKRAG
                     LSFAVFEASEAFSTVANFPKGKPIYTYPTGMTPEGDLQFRSEVHPKEQLLADLEARRK
                     EAGVEVTFARIERISRQGGVLLLHHGGGKTVTRARRAIVAIGRSGNHRRLGVPGEDSG
                     KVFNRLYDPKEFAGRQALVVGGGDSALETAIALGTAGAHVTLSYRKKEFSRPKPENVE
                     KVRKLERNPSAAVAVEKPSSERVTTATGDFMEKAGAHAPGSVRFAFGTQPMRIDPDSV
                     RLRDESGHETTIANDVVFTMLGREAPLEFFRRSGIPIRGEWKAATWVAFLSFLAFCVF
                     LYNWKAGGALNRWFQERRLFPYDVAAPSDPSTLAGTIAISARDPGFYYSIAYCLAVLL
                     FGVARIRRRRTPYVTAQTATLTAIQIVPLFLLPFVVLPLLGHNGVFDSGFGKTFADHL
                     FPAADASGHGREYWRAFGFILAWPLFIWNVFSPKPLAWWLVIAFVQTFVLIPLIIRRW
                     GKGAYCGWICSCGALAETMGDAYRQKMPHGPIWNRMNMTGQVILGAAFLLFFGRVLSW
                     SWPDSAVGHAAQKLYDGLLSGWSVLGIQLNYYHLVDVFLAGIVGVGM"
BASE COUNT          624 a         1411 c         1390 g          693 t
ORIGIN      
        1 cctcgacgac tcggcgaacc ggcgcatcgc gatccccgag gcgtttctga ccgcggacgc
       61 gatcctcgtc ctcctcgaga acgtcttctc gggactcgtc gtgcacgcgg acgtcgcgcg
      121 gcgacggctc gaagcggaga tcccgttcct tgcgaccgag aacatcctca tggaggcggt
      181 ccgccgaggc ggtgaccgcc aggagcttca cgagaagctg cgcgtattcg cgcgcgccgc
      241 ggccgaacgg cccgcgggca ccggacgggc gaccgatctg ctcgaccgga tcgccgcgga
      301 tgccgacttc ggtctgacac gcgcggagct cgaccaggcg gcgcgccccg agcgcctgac
      361 cggccggtcc tccgagcagg tcgagacgtt cgtgcgggaa gagctggacc cggctctcgc
      421 gggcgtcgaa gcggtcgcga gcgttcccct gaaggtgtga cgccgcgcga cgcggttcgg
      481 gtcgccctcc tggtcgccgt gctcggagcg ggcgcggcgt gtgcctcgca tcctcccgcg
      541 cgaagcgttt cgcacggccc cccggccccg ggatggacgg aggaaggggt cgcgtcgtgg
      601 tacggcggcc gcgacggctt cgaggggaag ccgacggcgt cgggcgagat ctacgactcg
      661 tcgctcctga ccgcggccca ccgcgagctc cctctcggaa cggtggtcga cgtgacgaac
      721 ctcgacaacg agcggaccgt ccgggtgcga atcaacgacc gcgggccgtt cgtcttcggg
      781 cgcgtgatcg atctctcccg cgccgcggcc gggaagatcg gactgatcgg gtccggaacc
      841 ggttccgtgc gcgtgaccgt ggtgacgccg ggcattccac tgcagatcac atccccgaca
      901 gggagatggg ccgtgcaggt cggatcgttc gccgaccagc tccgtgccga gcgacacgcg
      961 gaacggctgc gaggcaccgg ccgcgcggtg ttcctggagc cgtaccgcgg gctgacgcgc
     1021 gtgaaggtcg ggccgttcga cggccgcagg gaagcggagc acgagctcca gctgctcgaa
     1081 gacgacggct tcgaggggat cgtcgttccg acggactagc ctttcgtccg acgacaggag
     1141 cactgcaacc ccctcacccc gtccctctcc cggagggaga gggtgtgcgc tttctgtcct
     1201 ccagctcctc gagagtcttc ggccagtcgc ccttcaacag ctgggacagg gctcgatccg
     1261 cgaggagtct tccgccggtc tggcacgtcg ggcagtagtt cgactcgttc tcggcataga
     1321 cgatccgctg gatcttcgtc ccgcagaccg gacacggctt gccgaagcgg ccatggacgg
     1381 ccatgccttc gcgaaatgca gtgaccttct ctggaaacct ctttcgcgcc tcttcggtga
     1441 gtcggccggt ccagtcctcg aggacgtcgc gcgtcgcacc gtggaggcgg gagatttcct
     1501 ccgcggagag ccgcgacgtc agcttggcgg gcgacagccg cgcgcgatgg aggatctcgt
     1561 cggagtaagc gttgccgata cccgagaaga gccgcgggtc ggtcagcgtc cgcttcagcg
     1621 tatggttctc gcgcgtgagc gctgcgcgaa agctgtcccg gtccgcctcg agcacttcga
     1681 gccctccccg cgcatgctcc gccagggcgg cctcgccgcg gacgacgtgg agggacgccc
     1741 gcttcttcga gccagcctcg gtcaggagga gcgtgccgtc gagaaagtcg aacgccgcga
     1801 gcgagttctt cgccgccacc ggaaccgcct ccgtcttcca gtgaagccgg cctgcgatca
     1861 tgaggtggag gacgatgtgg aggtcgccct ccaggtcgaa aacgattcgc ttccccaggc
     1921 gtcgcagtcc gatgacgcgt cgtccttcga cctcggggag cggggggtcg accgagcgca
     1981 ggacgaacgg actcccgagc cgcacgcttt cgaggggctt ccccgccacg cgcgcctcga
     2041 tcgcgacgag gtaggcggca atgtcgggca actccggcat cgcggtggag aatagccggg
     2101 gatcgatgtc tttgctgagt cgctacacgc actggctcca cacccgctgg ccggcaggaa
     2161 ccgtcgagaa gctgccggac atccgtgaag acggcaccac cgccgtaccc ggcgtccggg
     2221 tggtcggcga cctcacgggc gtgccgctcc tgaagttctc ttccgattcc ggcgcacgcg
     2281 cggtccatgc gatcctgcgg gagcccgagt ttctccagac ccgcccccgg gagcccgatg
     2341 tcgtggacct cgcgatcgtc ggcgggggcg tttccggaat cgccgcggca atcgcagcga
     2401 agagggccgg cctctctttc gccgtcttcg aggcgagcga ggcgttttcg acggtcgcca
     2461 acttcccgaa gggcaagccg atctacacgt acccgaccgg catgacgccg gagggagacc
     2521 tgcagttccg atccgaggtc catcccaagg agcagctcct cgcagacctc gaggcgcggc
     2581 ggaaggaagc gggcgttgag gtgacgtttg cgcggatcga gcggatctcg cggcagggag
     2641 gcgtgctgct tctccatcac ggcggtggga agacggtcac ccgcgcccgc cgcgcgatcg
     2701 tcgcgatcgg aaggagcggg aatcaccgac ggctcggcgt tcccggagag gactccggca
     2761 aggtcttcaa ccgcctgtac gacccgaagg aattcgccgg gcggcaggca ctcgtcgtcg
     2821 gcggcggcga ctcagccctc gaaaccgcga tcgcgctcgg tacggcgggc gcgcacgtca
     2881 cgctgtccta tcggaaaaag gagttctcgc ggcccaagcc cgagaacgtc gaaaaggtcc
     2941 ggaaactcga gaggaacccg tctgccgcgg tcgccgtcga gaagccttct tccgaacgcg
     3001 tcacgaccgc caccggtgat ttcatggaga aggcgggagc gcacgcgccc ggctcggtgc
     3061 ggttcgcgtt cggcacacaa ccgatgcgga tcgacccgga cagcgtccgg ctgagagatg
     3121 agtccggcca cgagacgacg atcgccaacg acgtcgtctt cacgatgctc ggccgcgagg
     3181 cgccgctcga gttctttcgc cgctcgggca ttccgatccg cggcgaatgg aaagccgcga
     3241 cgtgggtggc tttcctctcg tttctggcct tctgcgtctt cctgtacaac tggaaggcgg
     3301 ggggcgcgct caaccgctgg ttccaggagc gtcgcctctt tccctatgac gtggcggcgc
     3361 cgagcgatcc ctcgacgctc gccggaacca tcgcgatctc ggcgcgcgat cccggcttct
     3421 actactcgat cgcttactgt ctcgccgtcc tcctcttcgg agtcgcgcgc atccggcggc
     3481 gccggacgcc ctacgtgacg gcgcagactg cgacgctgac ggcgatccag atcgtcccgc
     3541 tgttcctcct gcccttcgtc gtgctgccgc tgctcggaca caacggagtc ttcgactccg
     3601 gcttcggtaa gaccttcgcg gatcacctct tccccgcggc cgacgcctcg ggacacggcc
     3661 gcgagtactg gcgcgccttc ggcttcatcc tcgcgtggcc cctcttcatc tggaacgtct
     3721 tctcgccgaa gccgctcgcc tggtggctcg tgatcgcgtt cgttcagacg ttcgtgctca
     3781 ttcctctgat catccggcgg tggggcaagg gcgcctattg cggctggatc tgctcctgcg
     3841 gcgcgctcgc ggagacgatg ggggacgcct accggcagaa aatgccacac ggccccatct
     3901 ggaaccggat gaacatgacc ggacaggtca ttctcggcgc cgcgttcctt ctcttcttcg
     3961 ggcgtgtcct ctcgtggagc tggccggact cagcggtcgg tcacgccgcg cagaagctct
     4021 acgacgggct cctgtccggc tggtccgtcc tcgggatcca actcaattat taccacctcg
     4081 tggacgtctt cctcgccggg attgtcggcg tcggaatg
//
LOCUS       DASWAV010000244         1983 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U2665
            k141_493716, whole genome shotgun sequence.
ACCESSION   DASWAV010000244 DASWAV010000000
VERSION     DASWAV010000244.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960316
            Sequence Read Archive: SRR10260007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 1983)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1983)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:47:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,929
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1983
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_493716"
                     /isolate="SMAG_U2665"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.36444 N 132.31889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(196..>1983)
                     /locus_tag="VF958_05000"
     CDS             complement(196..>1983)
                     /locus_tag="VF958_05000"
                     /inference="COORDINATES: protein motif:HMM:NF014081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX9148503.1"
                     /translation="EIGGAKMTWLLPSILSAMLSAQPAGSDVSAARAVFEKNLDAIRR
                     RDKTAYLSCYWKSEKLARTGADGIALGYAAHEKSAGEMWPDTFDASDLDLVSVQPGIV
                     YGTYRYRVRYGADEQTGISERLFLKTPDGWKIAMTSAFPAAPGTPPPPRAIVGATLVD
                     GTGAAPVPNATVVLRGGKIDCAGRCAIPGGVTVVDARGMWVTPGLVDAHVHFSQTGWA
                     DGRPDALDVRSTHPYERVEADLKEHPDRFFRSQLCSGVTSVFDVGGYPWTVAMARHER
                     DDSRAPRVAAAGPMLSTIDFLANVPAERQLIYLKDADAARSGVRYIASLGSVAVKVLY
                     IVTPERGAAESEPVVLAAAEEARAAKLPLIVHATGLAEAKAALRVGAKVLVHSVNEQP
                     VDDEFLALAKKNGTIYCPTLTMFTGYGRLVQAVQSKKDLPPVDDPNDCVDGATLANVR
                     GTSGLDAKRLGPFLEMMAKHVSEFDQIGKANLKRVADAGIPIAMGTDAGNPLTLHGPS
                     VYAEMEAMQSAGMAPTQVIAASTRVGATAMGLEKEIGTVEKGKSADLLIVGGDPTADV
                     ANFRKVRYVVRAGVVRSIEELKATVAAAK"
BASE COUNT          303 a          712 c          619 g          349 t
ORIGIN      
        1 ccggttcgag cgagcttctc cgacttccag tagcaggaga gataagccgt cttgtcgcgc
       61 cgccggatcg cgtcgaggtt cttctcgaag actgcccggg cggccgagac gtcggagccc
      121 gcgggttggg ccggcagcac cgcgctcagg atggagagca ccagccacgt cattttcgct
      181 cctccgattt ccgtcttact tcgccgccgc caccgtcgcc ttgagctcct cgatcgaacg
      241 aacgaccccg gcccgcacga cgtaacgcac cttccggaag ttcgcgacgt cggccgtcgg
      301 gtccccgccc acgatcagga gatcggctga cttgcccttc tcgaccgtcc cgatctcctt
      361 ttccaacccc atcgccgtcg ccccgacgcg cgtcgaagcg gcgatcacct gcgtcggcgc
      421 cattcccgcc gactgcatcg cctccatctc cgcgtacacg gacggcccgt gcagcgtcag
      481 cggattgccg gcgtcggttc ccatcgcgat cggaatacca gcgtcggcaa cccgcttcag
      541 gttcgctttc cctatttgat cgaactccga aacgtgcttc gccatcatct ccaggaatgg
      601 gccgagtcgt ttggcatcga gcccggacgt cccgcgaacg tttgcgagtg tggccccgtc
      661 cacgcagtcg ttcgggtcgt ccaccggggg aagatccttc ttcgattgga ctgcctggac
      721 cagccggccg taaccggtga acatcgtcag cgtcgggcag tagatcgttc cgttcttctt
      781 cgcgagtgca aggaattcgt cgtccaccgg ttgctcgttc acgctgtgga caagaacctt
      841 cgctccgacg cgtagcgcgg ccttcgcctc agccagtccg gtcgcgtgga cgatgagcgg
      901 gagcttcgcg gctctcgcct cctctgccgc cgcgagcacg accggctccg actcggctgc
      961 gccgcgctcc ggcgtgacga tgtacaagac cttcaccgcg acggaaccga gcgaagcgat
     1021 ataccgcacc cccgaccgcg cggcatccgc gtccttcagg taaatcagct gccgctccgc
     1081 cgggacgttc gccagaaagt cgatcgtcga cagcatcggt cccgcggcgg cgacccgcgg
     1141 cgctctcgag tcgtcgcgct catggcgcgc catcgcgacc gtccacggat atccacccac
     1201 atcgaagacc gacgtcaccc ccgagcagag ctgagatcgg aagaatcggt cggggtgttc
     1261 cttcaggtca gcctcgaccc tctcgtaggg gtgcgtgctg cggacgtcga gggcgtccgg
     1321 ccggccgtcg gcccaacccg tctgggagaa gtgcacgtgc gcgtcgacga gcccgggcgt
     1381 cacccacatc ccgcgtgcgt ccacgaccgt cacgcctccc ggaatcgcgc agcgtcccgc
     1441 gcagtcgatc ttcccgccgc gcaggaccac ggtggcgttc ggcacgggcg ccgctcccgt
     1501 cccgtccacg agtgtcgcgc cgacgatggc gcgcggcggc ggcggcgtgc cgggcgccgc
     1561 ggggaacgcg ctcgtcatcg cgatcttcca gccgtcgggg gtcttcagga aaaggcgttc
     1621 ggagatcccg gtctgctcat ccgcgccgta ccgcacgcga taccggtacg ttccatagac
     1681 gatgcccggc tgaacggaga cgaggtcgag gtcgctcgcg tcgaaggtgt cgggccacat
     1741 ctctccggcc gacttctcgt gcgcggcgta gccgagcgcg atgccgtcgg caccggttcg
     1801 agcgagcttc tccgacttcc agtagcagga gagataagcc gtcttgtcgc gccgccggat
     1861 cgcgtcgagg ttcttctcga agactgcccg ggcggccgag acgtcggagc ccgcgggttg
     1921 ggccgacaac atcgcgctca ggatggaggg cagcagccac gtcattttcg ctcctccgat
     1981 ttc
//
LOCUS       DASWAV010000245         4621 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U2665
            k141_771970, whole genome shotgun sequence.
ACCESSION   DASWAV010000245 DASWAV010000000
VERSION     DASWAV010000245.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960316
            Sequence Read Archive: SRR10260007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 4621)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4621)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:47:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,929
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4621
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_771970"
                     /isolate="SMAG_U2665"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.36444 N 132.31889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..473)
                     /locus_tag="VF958_05005"
     CDS             complement(<1..473)
                     /locus_tag="VF958_05005"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF037785.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0009691 - cytokinin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LOG family protein"
                     /protein_id="HEX9148504.1"
                     /translation="MRPTRKVRREPDVGKIIDRTIVRLWETIEELEELTPAELHFYRV
                     AVFGSARIRTGDPEYAAVRELAGRLTQLGCDIVTGGGPGLMEAANEGAASSRGTRKTR
                     SFGLTISVPYEKTNPYLDTVTAHRTFFSRLHHFVRMSQAFVIFPGGVGTSLELLMV"
     gene            complement(517..1968)
                     /locus_tag="VF958_05010"
     CDS             complement(517..1968)
                     /locus_tag="VF958_05010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9148505.1"
                     /translation="MRPRDREGRVRKSLIFGALCLAIAAVAGAQIASDRSGASNWIDS
                     PYRNFGPPQALMAGRVVLLDEADDPNLKGLLSGEIPRLFTELYSRQGWRSPFAEGEPL
                     KIFVARKEAGGVRRVDSRSVENGRLVGAGILLDGSGFTSREIVREVARQIALATLAGY
                     RVSDGTFVTEAAAALISSSGDAEERDAVRQAAAAPELSIEASATVLGRLYLEELSRAA
                     GSDLLRAAWERAAETGQAVLPVLLGSYAERTGEGEDAPLVRFGARLYTAFEPEIAPSR
                     VALLDLESGALDAAAPAGFTLRHRTYVPGDGASALKVSWPIDGSAGAVVVRYRDTSLP
                     PDVLLLSAGDVRSIPLGGVERVDWVVAGGFSGGTLRAPAAIEPAAAFPYSGLAAHAGA
                     DPDGSRVWWTTASHDGLAGWAVFREEVLPDGRIARTGPEIIPSSKSAAESFAYAYLDP
                     TANPATYYRYTVWAVTEDGLLSRAFSVTLRSSD"
     gene            complement(2060..2863)
                     /locus_tag="VF958_05015"
     CDS             complement(2060..2863)
                     /locus_tag="VF958_05015"
                     /inference="COORDINATES: protein motif:HMM:NF033484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Stp1/IreP family PP2C-type Ser/Thr phosphatase"
                     /protein_id="HEX9148506.1"
                     /translation="MTSSINAFGLTDVGKKRKHNEDAYALDVNEGLFIVADGMGGHAA
                     GEVAAKITVETIGEFIAATRQKEEATWPFRYDHQLQFNSNRLAIAIEKANERVMAAVA
                     AQPWLKGMGTTVVAGLLNEKTLSLAHVGDSRAYLFRDGRLMRLTDDHSWVHEQVAAGI
                     LTEEEAKSHPLKNVVTRALGGGPSVSPDLQELTFQPGDEFLFCSDGLTTMLTDEEIME
                     AAEEEREPQNLCRKLVDTANERGGVDNITVVVVRVPDGTGQKARVPPPE"
     gene            complement(2945..3565)
                     /locus_tag="VF958_05020"
     CDS             complement(2945..3565)
                     /locus_tag="VF958_05020"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ domain-containing protein"
                     /protein_id="HEX9148507.1"
                     /translation="MFHSVDYYALLGVAREASESEIRERFRQLARESHPDRAPRENKA
                     EAEARFQELTEAVNVLCNPERRKAHDRAQVVALSSGASADMVLQDYLELGSSAYRAGQ
                     YVDAAGNFALAAQRNPKDVRAHHYLGLASARAGDLRAAVKALEAAVALDPHNAPLLKD
                     AGKIFRQAGLLAKAEKAFQDALRWDPSETDVRRALQEVRDERTVKS"
     gene            complement(3690..>4621)
                     /locus_tag="VF958_05025"
     CDS             complement(3690..>4621)
                     /locus_tag="VF958_05025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9148508.1"
                     /translation="ATLQAISPDRLAAFVKSGTARPEELDLLAHEAADPFVLEEIVRS
                     RSARDETLLYLAKTVTGRPQDALIANQARLLAEPALIRALFENPDLTADGHRLLTELT
                     EEFFEKRVRRRESEARSRESESAAAHEDLTPPEGADQDADLDLLDDREEASPEPSAAG
                     GEAPWDAEENSLFIGAIYKRLAAMNVSEKIKLAYTGTKEERRILVGDTNKLVGIAVLK
                     SRAISVNEAEAFAAMRNLDEEIYRRISWNREWMRKPAVVIALVRNPRAPLDIVLPLLK
                     RLSIRELRTVVRDRNLATVIRTTARRFLVQKRR"
BASE COUNT          752 a         1557 c         1510 g          802 t
ORIGIN      
        1 accatcaaga gctccaggct cgttccgacg ccgcccggga agatgacgaa tgcctgcgac
       61 atccggacga agtggtggag gcgggaaaag aacgtgcggt gagccgtgac cgtgtcgaga
      121 tagggattcg tcttctcgta aggaaccgaa atcgtcaacc cgaaggatcg ggtctttcga
      181 gtgccccggg aagaggcggc gccctcgttg gccgcctcca tcaaccccgg acctcccccg
      241 gtcacgatgt cgcagccgag ctgggtgagc cggcctgcga gttcccggac cgctgcgtac
      301 tcggggtcgc cggtgcgaat gcgcgcgctt ccgaaaaccg cgacgcggta gaaatgcaac
      361 tcggccggcg tgagctcctc gagctcctcg atcgtctccc agagccggac gatcgtgcgg
      421 tcgatgatct ttccaacgtc cggctcacgc cggacttttc tcgtgggtcg catggatggt
      481 cgcgaattcg ctccgaccgg ccgccgcgtg cgacggctaa tcggacgagc gcagcgtcac
      541 ggagaacgct cgcgagagca gcccgtcctc ggtgacagcc cacaccgtgt agcggtaata
      601 cgtcgccggg ttggcggtcg gatcgagata tgcgtaggca aacgactccg ccgcgctctt
      661 ggacgacgga atgatctcgg ggccggtccg cgcgatgcgt ccatcgggga ggacctcctc
      721 ccggaaaact gcccatcccg cgagaccgtc atgcgatgcc gtcgtccacc agacgcgaga
      781 gccgtccggg tcggcgccgg cgtgtgcggc cagcccggag tatggaaagg cggccgccgg
      841 ttcaatcgcg gccggcgccc gaagggtgcc tcccgaaaaa ccgcccgcta cgacccaatc
      901 gacccgctca accccgccca ggggaatcga ccggacgtcc ccggccgaaa ggaggagcac
      961 gtcgggagga agcgacgtat cccggtatcg gacgacgacc gcccccgcgc tgccgtcgat
     1021 cggccacgac actttgagcg ccgatgcccc gtcgccggga acgtacgtgc ggtgccgcag
     1081 cgtaaaccct gccggagcag ccgcgtcgag cgctcccgat tcgaggtcga gcaaggcgac
     1141 gcgggacggc gcgatttccg gctcgaaggc cgtgtagaga cgggctccga accggacgag
     1201 cggcgcgtcc tcgccttcac cagttcgttc cgcatagctc ccgagcagca ccggaagcac
     1261 cgcctgtccg gtctcggcag ccctctccca ggcggcgcgc agcagatcgg accctgccgc
     1321 gcgtgagagc tcctcgaggt aaagccggcc gagcaccgtt gcgctcgctt cgatcgagag
     1381 ctcgggcgcg gcggcggctt gtcggactgc atcgcgctct tccgcgtcgc cgctgctcga
     1441 aatcagggcg gccgcggctt ccgtcacaaa cgtcccgtcc gaaacgcgat agcccgcgag
     1501 cgtggcgagc gcgatctgcc gcgcgacctc acgcacgatc tcgcgtgacg tgaacccgct
     1561 gccgtccagc agaatcccgg cgcccacgag gcgaccgttc tcgacggagc gcgaatcgac
     1621 gcgccgaacg ccgcccgctt ctttccgcgc gacgaagatc ttcaacggtt cgccctcggc
     1681 gaagggtgac cgccagccct ggcgggagta gagctcggtg aaaagccgtg ggatctcgcc
     1741 ggagagaaga cccttgaggt tcgggtcgtc ggcctcgtcc aggagcacaa cccgtccggc
     1801 catgagcgcc tgggggggcc cgaaattgcg gtacggactg tcgatccagt tgcttgcacc
     1861 ggagcgatca gaggcgatct gggctcctgc cacggcggca atcgccaggc acaaggcgcc
     1921 gaaaatcagc gacttgcgaa cacgcccttc ccgatcacgc ggtcgcatgg aggttttagg
     1981 atagtcggcg agccgtggcc cgtcaatcgg tttacggatc gggtccggtt tccgtcgtcc
     2041 ttttttcccg atccgggagc tattccgggg gaggtaccct ggccttctgg ccggtaccgt
     2101 ccgggacccg gaccaccacg acggtaatgt tgtcgacgcc ccccctctcg ttggcggtgt
     2161 cgaccagctt ccggcagaga ttctgcggct cgcgctcctc ctcggccgcc tccatgatct
     2221 cctcgtcggt caacatcgtc gtcaggccgt cggaacagaa gaggaactcg tcgccgggct
     2281 ggaaggtcag ttcctgaagg tcgggcgaca cggacggacc gccaccgagg gcgcgggtca
     2341 cgacgttctt cagcggatgc gacttggctt cctcctccgt gaggatgccg gcggcgacct
     2401 gctcatgcac ccaggaatga tcgtccgtca accgcatgag tcgcccgtcc cggaagagat
     2461 acgcccggct gtcgccgacg tgcgcgaggg acagcgtctt ctcgttcaag agccccgcga
     2521 cgaccgtcgt tcccattccc ttcagccagg gttgcgcggc gacggccgcc atcactcgct
     2581 cgttcgcctt ttcgatcgcg atggcgagtc ggttgctgtt gaactgaagc tggtggtcat
     2641 accggaacgg ccacgtcgcc tcttccttct gccgcgtggc cgcaatgaac tcgccgatcg
     2701 tctcgaccgt gatcttcgcc gcgacttcgc cggccgcatg gccgcccatc ccgtccgcga
     2761 cgatgaagag cccttcgttc acatcgagcg cgtacgcgtc ctcgttgtgc tttcttttct
     2821 tgccgacgtc ggtcagcccg aaagcattga tcgaactcgt catctatccg cccgttatcg
     2881 cgaagtcagc ggccgatcgc cgcggaccgc aaacgcggcc ccgccgggtg tcgagacgag
     2941 gcgctcaaga cttgaccgtc cgctcgtcgc ggacttcctg cagagcccgc cgcacatcgg
     3001 tttccgaggg gtcccaccgt agagcatcct gaaaggcctt ttccgctttt gcgagaagtc
     3061 ccgcctgtcg gaagatcttc ccggcgtctt tcaacagcgg cgcattgtgg ggatcgagag
     3121 cgacggccgc ctcgagggcc ttgaccgccg ccctcaggtc acccgcccgc gccgacgcga
     3181 ggccgaggta gtgatgagct cgaacgtcct tcggattgcg ttgcgctgcg agggcgaagt
     3241 tgccggcggc atccacgtat tggcccgccc tgtaggcgga cgacccgagc tccaggtagt
     3301 cctggagaac catgtcggcg gaggccccgg atgagagcgc aacgacttgc gcccgatcgt
     3361 gcgccttccg gcgttccggg ttgcaaagga cgttcacggc ttcggtcagc tcctgaaacc
     3421 tcgcttcggc ttccgccttg ttctcccggg gagcgcgatc ggggtgggac tcgcgggcga
     3481 gctggcggaa tcgctcacgg atttcgcttt cgctcgcttc gcgggccacg ccgagcaacg
     3541 catagtagtc gacgctgtgg aacatgtctt ccgaaaaatg gaccgggttc ggctccggac
     3601 tacgataagg gaacccgttc gccggagcaa gcgaaacgga tgtgcggcgg atcactttct
     3661 cgcctcgacg agcaggccgg cctgagacct cagcggcgct tctggacgag gaagcggcgg
     3721 gcggtcgtgc ggatcaccgt tgcgaggttc cggtcgcgta cgacggtacg aagctcccgg
     3781 atcgacaggc gcttcagcag cgggagcacg atgtcgaggg gcgccctcgg gttgcggacg
     3841 agagcgatga cgacggcggg cttcctcatc cactcccggt tccagctgat ccggcggtaa
     3901 atctcctcgt ccaggttgcg catcgcggcg aatgcttccg cttcgttgac cgaaatcgcg
     3961 cgagacttca ggactgcgat cccgaccagc ttgttcgtgt caccaacgag gatccgccgc
     4021 tcttccttgg tccccgtgta ggcgagcttg attttctcgc tcacgttcat cgcggcgagg
     4081 cgtttgtaga tggcaccgat gaagagggaa ttctcctccg cgtcccaagg agcttcccct
     4141 ccggcggccg acggctcggg actcgcctct tcgcggtcgt caaggagatc gaggtccgca
     4201 tcctggtcgg cgccttcggg cggcgtcagg tcctcgtgcg ccgcggcgct ctctgactcc
     4261 cggctccgcg cttccgactc ccggcgccgc acgcgcttct cgaagaactc ctcggtaagc
     4321 tcggtgagaa gccgatgccc gtccgccgtc aggtccggat tctcgaacag cgcgcgtatc
     4381 agcgccggct cggcgaggag acgtgcctgg ttggcgatca gggcgtcttg cggacggccg
     4441 gtcaccgtct tcgcgaggta gagcagagtc tcgtcacgag cggatcgcga gcgcacgatc
     4501 tcctcgagga cgaacgggtc cgccgcttcg tgggcgagga gatcgagctc ctccggacgg
     4561 gccgttcccg acttgacgaa cgccgcgagt cggtccgggg agatcgcctg cagggtggcc
     4621 c
//
LOCUS       DASWAV010000246         2627 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U2665
            k141_570772, whole genome shotgun sequence.
ACCESSION   DASWAV010000246 DASWAV010000000
VERSION     DASWAV010000246.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960316
            Sequence Read Archive: SRR10260007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2627)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2627)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:47:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,929
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2627
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_570772"
                     /isolate="SMAG_U2665"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.36444 N 132.31889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..663)
                     /locus_tag="VF958_05030"
     CDS             complement(<1..663)
                     /locus_tag="VF958_05030"
                     /EC_number="4.2.2.n1"
                     /inference="COORDINATES: protein motif:HMM:NF013618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HEX9148509.1"
                     /translation="MPRFATFAAVGLAAALFAAPAGAELIVFSDGRVTKVAGYRVDGD
                     EVEIQVRGGGSYRVDASLVERIVEDEVEVSEVEVRSIGFDEKVAYDLSYKEERKPLFS
                     SAYDGLIAAEAKKHGVDAALVSAVIRAESNYEPRALSRKGARGLMQLMPATARRLEVR
                     KPFDPASNVAGGVKYLKELAERFHHRPELVLAAYNAGETAVETYGGVPPYRETIGYVK
                     KIL"
     gene            complement(708..1670)
                     /gene="xerD"
                     /locus_tag="VF958_05035"
     CDS             complement(708..1670)
                     /gene="xerD"
                     /locus_tag="VF958_05035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020649027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009009 - site-specific recombinase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HEX9148510.1"
                     /translation="MTGKRRPSSSSPSTSDFLARTLPTYLDHLAVERGLSRASVDAYR
                     RDLEAFGRWMSTARTTATKARRDHLVTYLRHLRTRGLSSRSASRALSAVRGFYRFATA
                     HLDFAGDPTDDLSNPKAGFALPKTLTEKEVESLLAAPDVALPFGLRDRAMLELLYASG
                     LRVSELVQLPRDAVDLEAGILRVSGKGGRERLVPFGASAGDWVRRYLGEGRPALDRKG
                     RAELFLSARGAGMTRQRFWQLIEGYGRKAGIRRKLAPHAVRHAFATHLLDRGADLRAL
                     QMMLGHADISTTQIYTRVSAGRLRSVYDRYHPRARSAGRGKMKP"
     gene            complement(1667..>2627)
                     /locus_tag="VF958_05040"
     CDS             complement(1667..>2627)
                     /locus_tag="VF958_05040"
                     /inference="COORDINATES: protein motif:HMM:NF012885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HEX9148511.1"
                     /translation="CAARTVEADILPIIEVSQKVGIPIEVSCFIGSSPIRQYAEDWDL
                     DRMLRLSENAVSLAVRAGLPVMYVTEDTIRAHPDHIRAMYTTALRAGAQRICVCDTVG
                     HATPNGVQNLIRFVSNLVDEVSPEAKIDWHGHQDRGFGLQNALWALEAGAHRVHACAI
                     GIGERVGNTPMDQLLVNLQLLGWIDRDLTKLHEYCELASRATGVPIPDNYPIVGKDAF
                     RTGTGVHAAAIIKARKKGDDWLADRVYSGVPASMVGRKQIIEVGPMSGISNVQCWLDD
                     HGIPARADLVDAIFQRAKESDRILTDDEILALVHGLTPAAASR"
BASE COUNT          411 a          884 c          910 g          422 t
ORIGIN      
        1 caggatcttc ttcacgtaac cgatcgtctc ccggtagggg ggcactcccc cgtacgtctc
       61 caccgccgtc tcgccggcgt tgtacgcagc gagcacgagc tcgggccggt ggtggaaacg
      121 ctcggcgagc tctttgaggt atttcacgcc gcccgcgacg ttcgacgcgg gatcgaaggg
      181 tttgcggact tccaggcgcc gggcggtcgc cggcatgagc tgcatgagac cgcgagctcc
      241 cttgcgggac agagccctcg gctcgtagtt cgattccgcg cggatcaccg cggagacgag
      301 cgccgcgtcc acgccgtgct tcttcgcctc ggcggcgatc agcccgtcgt aggcgctcga
      361 gaagagcggc ttgcgctcct ccttgtacga gaggtcgtaa gcgaccttct catcgaagcc
      421 gatcgagcgg acttcgacct ccgagacctc gacctcgtct tcgacgatcc gttcgacgag
      481 cgacgcgtcg acccggtagc tgccgccgcc ccgcacctgg atttccactt cgtcgccgtc
      541 cacccggtag ccggccacct tggtgacgcg accgtcggaa aagacgatga gctcggcccc
      601 cgccggggcg gcaaagagag ccgccgccag tccgaccgct gcaaacgtgg cgaaacgggg
      661 cattcggctc gtgcaactgc cccgtccggc ccggaattcc cccgtggtca cggcttcatt
      721 ttgccacgtc cggccgaccg ggccctcggg tgatatcggt cgtagaccga ccgcagccgc
      781 ccggccgaaa cgcgcgtata gatctgagtc gtcgagatat ccgcgtgtcc gagcatcatc
      841 tgcagggccc gcaggtctgc gccccgatcg aggagatgtg tggcgaaagc gtgccggacg
      901 gcgtgtgggg cgagcttgcg ccggatgccg gccttccggc cgtacccctc gatcaactgc
      961 cagaaccgct gccgggtcat cccggcgccc cgtgccgaga ggaagagctc ggcccgcccc
     1021 ttccggtcga gggcgggacg cccttcgccg aggtaacggc ggacccagtc gcctgcggaa
     1081 gccccgaacg gcacgagccg ctcgcgcccc ccttttccgc tgacccgcag gatccccgct
     1141 tcgaggtcca cggcgtcgcg cgggagctgc acgagctcgg acacccgcaa gccggaggcg
     1201 tagaggagct cgagcatggc ccgatcgcgc aggccgaacg ggagtgcgac gtcgggagcc
     1261 gcgagcaggc tctcgacctc tttctccgtg agcgtcttcg gcagcgcgaa accggccttc
     1321 gggttcgaga ggtcgtccgt gggatcgccc gcgaaatcga ggtgagcagt cgcgaagcga
     1381 tagaagccgc ggaccgcgga gagcgcccgc gacgccgacc gcgaggagag accgcgcgtc
     1441 cgcaggtgcc gaagatacgt gacgaggtga tcgcgccgcg ccttcgtcgc cgtcgttcgc
     1501 gcggtggaca tccagcggcc gaatgcctcg aggtcgcggc ggtaggcgtc cacgctcgcg
     1561 cgggaaaggc ccctctcgac ggcgaggtgg tcgaggtagg tcgggagagt gcgcgcgagg
     1621 aagtcgctgg tcgaaggaga agaggaggag ggtcgcctct tgccggtcag cgcgaggccg
     1681 ccgcgggggt gagcccgtgc acgagggcga ggatctcgtc gtcggtcagg atccgatcgc
     1741 tctccttggc gcgctggaag atcgcgtcga cgaggtcggc gcgcgcgggg attccatgat
     1801 cgtcgagcca gcactgcacg ttcgagatcc cgctcatcgg gcccacctcg atgatctgct
     1861 tccggccgac catgctcgcc ggaacgcccg agtagacccg gtcggccagc cagtcgtccc
     1921 ccttcttgcg ggccttgatg atcgccgcgg cgtggacgcc ggttcccgtc cggaaggcgt
     1981 ccttcccgac gatcgggtag ttgtcgggga tcggcacgcc ggtcgctcgc gaagcgagct
     2041 cacaatactc gtgcagtttc gtcaggtcgc gatcgatcca cccgagcagc tgcaggttca
     2101 cgaggagctg gtccatcggc gtgttgccga cgcgctcgcc gatcccgatc gcgcacgcgt
     2161 ggacgcgatg cgcgccggcc tcgagcgccc acagggcgtt ctggaggccg aacccgcggt
     2221 cctggtggcc atgccagtcg atcttcgctt cggggctcac ctcgtcgacg aggttggaca
     2281 cgaagcggat caggttctgg acaccgttgg gggtcgcgtg cccgaccgtg tcgcaaacgc
     2341 agattcgctg tgcgccggcc cgaagcgcgg tcgtgtacat cgcgcggatg tggtcggggt
     2401 gtgcgcggat cgtgtcttcg gtcacgtaca tgacgggaag ccctgcgcgc acggcaaggg
     2461 aaacggcgtt ctcggacagg cgcagcattc ggtcgaggtc ccagtcctcg gcgtattgcc
     2521 ggatcggtga cgacccgatg aagcaggaga cctcgatcgg aatgccaacc ttctgggaga
     2581 cctcgatgat cggaagaatg tccgcctcga ccgtccgcgc cgcgcaa
//
LOCUS       DASWAV010000247         4207 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U2665
            k141_772026, whole genome shotgun sequence.
ACCESSION   DASWAV010000247 DASWAV010000000
VERSION     DASWAV010000247.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960316
            Sequence Read Archive: SRR10260007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 4207)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4207)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:47:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,929
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4207
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_772026"
                     /isolate="SMAG_U2665"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.36444 N 132.31889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(105..653)
                     /locus_tag="VF958_05045"
     CDS             complement(105..653)
                     /locus_tag="VF958_05045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9148512.1"
                     /translation="MTVICAAAALRATLQAHAEREYSRTGSARWIWYSREVKEPRELA
                     FVATRDVVLERSPERATAKLFGDEWHVLWVNGRRVGGARQHPGDPLALYEVAGYLRRG
                     VNRIAIECGSKTGVGGLLFSLDLSDGGRDAVVSDGRWRVDSSRQAIFSGGRYRAEVWG
                     SPPMYPWGWPRLPRPNELRSAL"
     gene            769..1518
                     /gene="kdsB"
                     /locus_tag="VF958_05050"
     CDS             769..1518
                     /gene="kdsB"
                     /locus_tag="VF958_05050"
                     /EC_number="2.7.7.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018702913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008690 - 3-deoxy-manno-octulosonate
                     cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate cytidylyltransferase"
                     /protein_id="HEX9148513.1"
                     /translation="MKVIGVIPARYASIRFPGKPLAPLAGRPMVLHVLAAARAARTLD
                     KVLVATDDSRIADVVRREGGEAILTSADAASGTDRLAEAAGRVAADVYVNVQGDEPLM
                     PAENIDLAVETLLAGAGREIATVALPMEPEGASDPNVVKVVAAADGRALYFSRAPIPY
                     PRSGDPAYRKHLGLYAYRAETLAKLAVLAPSPLERTESLEQLRWLEAGFTVWVGEAQA
                     DSIGVDTPEDLAKAEEVLERAGKKLEKEVVR"
     gene            1515..3179
                     /locus_tag="VF958_05055"
     CDS             1515..3179
                     /locus_tag="VF958_05055"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013569783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006241 - CTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HEX9148514.1"
                     /translation="MSAKYVFVTGGVVSSLGKGIAAASIGALLEARGFKVALAKFDPY
                     INVDPGTMSPFQHGEVYVTDDGAETDLDLGHYERFTSMTGSRVNNTTAGRIYATVIER
                     ERRGDYLGKTVQVVPHITDEIKESIRKVGPDADVVLIEIGGTVGDIESLPFLEAIRQM
                     RQEAGRGNAVNVHLTLVPYIKASDELKTKPTQHSVKDLLSIGIQPDVLLCRTDRPLPP
                     DVKRKISLFCNVPVEAVIMAKDVPSIYEVPLQFAAEGLDEILLDLLNLPHYDRDLSKW
                     ENLVNRIKNPSGEVEIAVVGKYVDYGDSYKSLNEALAHGGIANNVRVKLQYVDAETLE
                     NEQYPEALVRADAVLVPGGFGRRGVEGMMRAIQYAREKKVPFFGICLGLQTAVIEYAR
                     HVAGLEGANSTEFEEEPKYKVIFKLRDLIGVEALGGTMRLGKYPCELSSGSFAARAYG
                     ESVISERHRHRYEVNQEFVPTLREAGMSFTGMSPDKKFVEIIELHADHPWFLGCQFHP
                     ELKSKPFACHPLFRDFIRAAKAHRGAREQIAAAEAIPVRVRGEESA"
     gene            3218..4012
                     /gene="kdsA"
                     /locus_tag="VF958_05060"
     CDS             3218..4012
                     /gene="kdsA"
                     /locus_tag="VF958_05060"
                     /EC_number="2.5.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009144993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008676 - 3-deoxy-8-phosphooctulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-8-phosphooctulonate synthase"
                     /protein_id="HEX9148515.1"
                     /translation="MGGGAPPLFFAGPCVIESRAHAVAMGKRLAEISQETGAKIVYKS
                     SYDKANRSSRDSFRGPGLAEGLDILAEVRATTGLPLLSDVHAPDEAEAAAKVLDVLQI
                     PAFLSRQTDLLVAAARTGKPLNIKKGQFLAPDDMDKVVEKCRAAGNEKLMLCERGASF
                     GYHDLVVDMRAFQIMRRLGYPIVYDATHSLQLPGGGKETGGLKQYAPVLARAAAATGC
                     VDGFFLEVHDDPDRALSDSSTQLDVETLAELIRTVTALWNVARGTS"
BASE COUNT          742 a         1389 c         1390 g          686 t
ORIGIN      
        1 tatcgcaagg tgatcaccct ctccggctac gacgctgaca acccgtactg cgccgcagcg
       61 cgagaggcgc tcgacgcgct cgtgaagaac ttcgaggaag acgcctagag agcgcttcgg
      121 agttcgtttg gccgcggcag acgaggccag ccccacggat acatcggtgg cgagccccag
      181 acctcggcgc ggtaacgacc gccggagaat attgcctgcc gggaggagtc gacgcgccaa
      241 cggccgtccg aaacgaccgc gtcgcgcccc ccgtcggaca ggtcgagcga gaacagcagc
      301 cctccaaccc ccgttttgct tccgcactcg atcgcgatcc ggttgacccc ccgccgcaga
      361 tatcccgcca cttcgtacag cgcaagtgga tctcccgggt gctgccgcgc tcctcccacg
      421 cggcggccgt tcacccagag cacgtgccat tcgtcgccga acaactttgc cgtcgcgcgc
      481 tccggtgagc gctcgagaac gacgtcgcgg gtcgcgacga aagcgagctc gcggggctcc
      541 ttgacttcgc gcgagtacca gatccaccgg gcgcttccgg ttctcgagta ttcccgttcc
      601 gcgtgcgcct gcagggtcgc gcgaagtgcc gcggccgcgc aaatgaccgt cacgagcacg
      661 tacaaccacg caaatgacgc ccgcgaccag gcgccgcgat tcggacgggg gacggccgtt
      721 ccttgaccga tcgtctgcgg cacgctactc tatagccccg atgtcttcat gaaagtcatc
      781 ggagtgattc ccgcccgcta cgcctcgatc cgattccccg gaaagcctct cgctcccctt
      841 gccggccggc cgatggtcct gcacgtcctg gcggccgcgc gcgcggcgcg gacgctcgac
      901 aaagtactcg tcgcgaccga cgactctcgc atcgcggacg tggtgcgccg ggaaggggga
      961 gaagcgatcc tcacttcggc cgacgcggcg agcggaaccg atcgcctggc ggaagcggcg
     1021 gggcgggtgg cggcggacgt ctacgtgaac gttcaggggg acgagcctct catgcctgcg
     1081 gagaacatcg atctggccgt ggaaacgctg ctggccggcg ccgggcggga aattgcgact
     1141 gtcgctcttc cgatggagcc ggaaggagcc agcgatccga acgtggtgaa ggtcgtggcg
     1201 gcggcggacg ggcgcgctct ctacttctcg cgagcgccga tcccttaccc gcggtccggc
     1261 gatccggcgt atcgcaagca cctcggtctc tacgcgtacc gggcggagac actggcgaag
     1321 ctcgccgtcc ttgcgccctc accgctcgag cggacggagt cgctcgagca actgcgctgg
     1381 ctggaggcag gcttcacggt ctgggtcgga gaggctcagg cagactcgat cggcgtggat
     1441 acgccggaag acctggcgaa ggcggaagaa gtgctggaac gggccggaaa gaaactggaa
     1501 aaggaggtgg ttcgatgagc gcgaaatacg ttttcgtcac cggcggggtc gtctcgtcgc
     1561 tcggcaaggg gatcgcggcc gcgtccatcg gggcgttgct cgaggcgcgc ggtttcaagg
     1621 tcgcgctcgc gaagttcgac ccgtacatca acgtcgaccc cggaacgatg tcgcctttcc
     1681 agcacggcga ggtttacgtc accgacgacg gagccgagac cgacctcgac ctcggacatt
     1741 acgagcggtt cacgtcgatg accgggtccc gggtgaacaa caccaccgcc ggccggatct
     1801 acgcaaccgt catcgagcgg gagcgccgcg gcgactacct cggcaaaacg gttcaggtcg
     1861 tccctcacat caccgacgag atcaaggagt cgatccggaa ggtcggaccg gacgcggacg
     1921 tcgtgctgat cgagatcggg ggcacggtcg gcgacatcga gtcccttccg ttcctcgagg
     1981 cgatccggca gatgcgccag gaggcggggc gcggcaacgc cgtgaacgtt cacttgacgc
     2041 tcgtgccgta catcaaggct tccgacgaac tgaaaacgaa gccgacacag cactccgtca
     2101 aggatcttct ctcgatcggg atccagccgg acgtcctgct ctgccggacg gatcgcccgc
     2161 tgccgccgga cgtgaagagg aagatctcgc tcttctgcaa cgtcccggtc gaagcagtga
     2221 tcatggcgaa ggacgttccg tcgatctacg aggtcccgct ccagttcgcg gccgaggggc
     2281 tcgacgagat cctgctcgac ctgctgaacc tgccgcacta cgaccgcgac ctttcgaaat
     2341 gggagaacct ggtcaaccgg atcaagaacc cgtcggggga ggtcgagatc gcggtcgtcg
     2401 gcaagtacgt ggactacggc gactcctaca agtccttgaa cgaggcgctc gcgcacggcg
     2461 ggatcgcgaa caacgtgcgc gtcaagctcc agtacgtcga tgccgagacc ctcgaaaacg
     2521 agcagtatcc cgaggcgctc gtccgcgccg acgccgtgct cgtcccggga gggttcggtc
     2581 ggcgcggcgt cgaaggaatg atgcgcgcca ttcagtacgc tcgcgagaaa aaggtcccgt
     2641 tcttcgggat ctgcctcggg ttgcagaccg cggtcatcga gtacgcgcgg cacgtagcag
     2701 gactcgaagg agcgaattcg acggagttcg aagaggagcc gaagtacaag gtcatcttca
     2761 agctgcgcga cctgatcgga gtcgaggccc tcggcggcac gatgaggctc gggaagtatc
     2821 cctgcgagct ctcgtccggg tcgtttgcag cgcgcgctta cggggaaagc gtgatctcgg
     2881 aacggcatcg gcaccggtac gaggtgaacc aggagttcgt tccgactttg agggaagccg
     2941 ggatgtcgtt caccggcatg tcgcccgaca agaagttcgt cgagatcatc gagctgcacg
     3001 ccgaccaccc gtggttcctc ggctgccagt tccacccgga gctgaagagc aagccgttcg
     3061 cgtgtcaccc gctcttccgt gacttcatcc gcgcggcgaa ggcccaccgc ggggcgcggg
     3121 agcagatcgc ggcggccgaa gcgattccgg ttcgggtgcg cggagaggag agcgcctgac
     3181 gccggtccgc tcggtcccga ttgcgcccgg ttactcgatc ggcggcggcg cgcctcccct
     3241 gttcttcgcc gggccgtgcg tcatcgagtc gcgcgcccac gctgtcgcca tgggaaagcg
     3301 cctcgctgag atctcgcagg agaccggcgc gaagatcgtc tacaagtcgt cctacgacaa
     3361 ggcgaaccgg tcctcgcgcg actcgttccg cgggccgggt ctcgccgaag ggctcgacat
     3421 cctcgcggaa gtcagagcga caacggggct tcccctcctc tccgacgtcc acgcgccgga
     3481 tgaggcggag gcggcggcga aggtcctcga cgtcctgcag attccggctt ttctttctcg
     3541 ccagacggat ctcctcgtcg cggccgctcg cacgggcaag ccgttgaaca tcaagaaggg
     3601 ccagtttctc gcccccgacg atatggacaa ggtcgtcgag aagtgccgcg ccgccggcaa
     3661 cgagaagctc atgctctgtg agcgcggagc gtcattcggg tatcacgacc tcgtcgtgga
     3721 catgcgcgcg ttccagatca tgcgccggct cgggtatccg atcgtctacg acgcgactca
     3781 ctcgctccag ctccccggcg gcgggaagga aaccggcggc ttgaagcagt acgcgcctgt
     3841 ccttgcgagg gctgcggccg cgacgggctg tgtcgatggc ttctttctcg aagtgcacga
     3901 cgatccggac cgcgcgctct ccgactcgtc gacccagctc gacgtcgaaa cgcttgcgga
     3961 gctgatccgg acggtgaccg cgctctggaa cgtcgcgcgc ggaacttcct aggaggcctt
     4021 ctcgaacacc tcgacgtcgt agccggcgtc gatccacgcc tcgagtccgc ccgcgagcgg
     4081 ccgcactttc ttgtatcccc tctcgatcag catgcgggcc acacgggccg ccgacgcctc
     4141 gttgggtcag gtgcagtaga ggacgacctc gacgtccggc cgtatgccgg gcagtttcgc
     4201 gtcgagg
//
LOCUS       DASWAV010000248         2249 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U2665
            k141_613643, whole genome shotgun sequence.
ACCESSION   DASWAV010000248 DASWAV010000000
VERSION     DASWAV010000248.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960316
            Sequence Read Archive: SRR10260007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2249)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2249)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:47:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,929
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2249
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_613643"
                     /isolate="SMAG_U2665"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.36444 N 132.31889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1218
                     /locus_tag="VF958_05065"
     CDS             <1..1218
                     /locus_tag="VF958_05065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012098652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9148516.1"
                     /translation="EVKPAAVKDSDQSAQPAASKVTNAREPDTRYKTLLDTFRAVKKV
                     DPYSPTAPTLIARRFEENRELPEARVQKMFEDVLTSPLVPRVAKVIERRLGRPLEPFD
                     VWYSGFKQRGKYTEAQLDEITRKKYPTPEAYGKDIPNLLQKLGFSKERAEYVANHIEV
                     HPARGSGHAFGAGKRGFQAYLRTRVEKDGMNYKGYNIAVHEMGHNVEQTFSLYDIDHY
                     LLSGVPNTAFTEALAFVFQGHDLELLGLVQPDAKSRALDVLNDFWGTYEIAGVGLVDM
                     AVWHWMYDHPTATPGELREATVGIAKKFWNRYYAPVFGKKDVVLLGIYSHMIDSILYL
                     PDYPIGHMIAFQVERQMEKAGAIGPEFERITKTGSVAPDLWMKKATGAPVGPEALLEA
                     TGKALPEIEAARD"
     gene            1348..>2249
                     /locus_tag="VF958_05070"
     CDS             1348..>2249
                     /locus_tag="VF958_05070"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEX9148517.1"
                     /translation="MPTGLATPSAVAAAQRRVHSSDPIGRLASSESLEPGATTPGSVI
                     GGRYRIIGLIGRGGMGEVYRADDLKLGQPVALKFLPEKLASDPAWIERFYAEVRHARG
                     VSHPNVCRVYDVGEIEGRHFLSMEYVDGEELASLLRRIGRLPPDKAVEIARELCAGLA
                     AAHDKGVLHRDLKPANVMIDGRGRAKITDFGLAVGVEDEKGGAEVSGTPAYMAPEQLS
                     GKGASVQSDIYALGLVLYELFTGKRAFEGKSFVELLRKHENEAPKSPGELVSDLDPRV
                     ESVILRCLEKDPRRRPASALAVAAS"
BASE COUNT          400 a          738 c          762 g          349 t
ORIGIN      
        1 gaagtcaagc ccgccgccgt gaaggactcg gaccaatcgg cgcagccggc cgcctcgaag
       61 gtgacgaacg cgcgcgagcc cgacacgcgt tacaagacgc tcctcgatac gttccgcgcg
      121 gtgaagaagg tcgatcccta ctcgcccacc gctccaacgc tgatcgcgcg ccgattcgag
      181 gagaaccgcg agctgccgga ggcgcgcgtc cagaagatgt tcgaagacgt gctgacgtcg
      241 cccctcgtgc ctcgcgtcgc gaaggtgatc gagagacggc tcgggcggcc gctcgagccc
      301 ttcgacgtct ggtactcggg gttcaagcag cgcggcaagt acaccgaggc gcagctggac
      361 gagatcacgc gcaagaagta cccgacgccg gaggcctacg ggaaggacat cccgaatctt
      421 ctccagaagc tcggcttttc gaaggagcgg gccgagtacg tcgcgaacca catcgaggtc
      481 catcccgcgc gcggctccgg tcacgccttt ggcgccggca agcgcgggtt ccaggcatac
      541 ctgcgcacgc gcgtcgagaa ggacgggatg aactacaagg gctacaacat cgccgttcac
      601 gagatgggcc acaacgtcga gcagaccttc tcgctctacg acatcgatca ctacctgctt
      661 tccggcgtcc cgaacaccgc tttcaccgag gcgctcgcgt tcgtcttcca ggggcacgac
      721 ctcgagctcc tcggactcgt gcaacccgac gcgaagagcc gcgcgctcga cgtcctgaac
      781 gacttctggg gaacgtacga gatcgcgggg gtcggcttgg tcgacatggc cgtatggcac
      841 tggatgtacg accatccgac cgcgacgccg ggggagctgc gtgaggcgac cgtcggcatc
      901 gcgaagaagt tctggaaccg gtactacgcg ccggtcttcg ggaagaagga cgtcgtgctg
      961 ctcgggatct actcccacat gatcgactcg atcctctact tgcccgacta cccgatcggc
     1021 cacatgatcg cgttccaggt cgagcggcag atggagaagg ccggcgcgat cggccccgag
     1081 ttcgagcgga tcacgaagac gggaagcgtg gcgcccgatc tctggatgaa gaaggcgacc
     1141 ggcgcgccgg tgggaccgga ggcgctgctc gaagcgacgg ggaaggcgct gccggagatc
     1201 gaggccgcga gagactaacg gtcgcctgaa gtaccatcga tttcaggcga cggcgttcca
     1261 atgaaatgcg gctcgtgctc ggccgaggtg cgggaggact cccgcttctg ctcctcgtgc
     1321 ggctcgccgg tgagctcgga ctcgcaaatg ccgaccgggc tcgcgacgcc ctctgccgtc
     1381 gctgccgcgc agcggcgggt ccactcttcg gatccgatcg gacgtctggc gtcctccgaa
     1441 tcgttggaac cgggcgcgac gacgccggga agtgtgatcg gcggacgcta tcggatcatc
     1501 ggcttgatcg gccgcggcgg aatgggcgag gtgtaccgag cggatgatct gaagctcggc
     1561 cagccggtcg cgctgaagtt cctgccggaa aagctcgcat cggatcccgc ctggatcgag
     1621 cggttttatg ccgaggtgcg gcacgcgcgg ggcgtctcgc atccgaacgt ctgccgcgtc
     1681 tatgacgtcg gcgagatcga aggccgccac ttcctctcca tggagtacgt ggacggcgaa
     1741 gagctggctt cgctcctgcg gcgcatcggg cgcctcccgc cggacaaggc ggtcgagatc
     1801 gcgcgcgagc tctgtgccgg acttgccgcc gcgcacgaca agggggtcct ccaccgggac
     1861 ttgaagcccg ctaacgtcat gatcgacggc cgaggccgcg cgaagatcac cgacttcggc
     1921 ctcgcggtcg gcgtcgagga cgaaaagggc ggtgcggagg tctcgggcac gcccgcctac
     1981 atggcgcccg agcagctttc cggcaagggc gcttccgtcc agagcgacat ctacgcgctc
     2041 ggcctcgtgc tctacgagct gttcaccggc aagcgcgcct tcgagggcaa gagcttcgtc
     2101 gagctgttgc gcaagcacga gaacgaagcc cccaagagcc cgggcgagct cgtgagcgac
     2161 ctcgacccca gggtcgagtc ggtgatcctg cgctgtctcg agaaggaccc gcggcggcgc
     2221 ccggcctccg ccttggccgt cgcggcgtc
//
LOCUS       DASWAV010000249         4825 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U2665
            k141_413910, whole genome shotgun sequence.
ACCESSION   DASWAV010000249 DASWAV010000000
VERSION     DASWAV010000249.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960316
            Sequence Read Archive: SRR10260007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 4825)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4825)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:47:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,929
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4825
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_413910"
                     /isolate="SMAG_U2665"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.36444 N 132.31889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..228
                     /locus_tag="VF958_05075"
     CDS             <1..228
                     /locus_tag="VF958_05075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018006960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BBE domain-containing protein"
                     /protein_id="HEX9148518.1"
                     /translation="WEDPADDKRVIAWARDFFNASAPFASGGVYVNFLTADEGDRVRS
                     AYGPNYDRLAQVKRKYDPKNLFRVNQNIVPA"
     gene            281..784
                     /locus_tag="VF958_05080"
     CDS             281..784
                     /locus_tag="VF958_05080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019401567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TspO/MBR family protein"
                     /protein_id="HEX9148519.1"
                     /translation="MSPVSPEPPVFSRPGLITVLPFLVAVAATAFIAGRFRPGAWYEA
                     LAKPSWTPPGWLFAPVWFLLYLGIAVAGWILWRAAGSGASLPLALWAAQLVLNAAWSW
                     LFFGLHRPGAALVDIVLLLVLILAFALTAHPFSPGAAWLFVPYGLWVLFATALNTAIV
                     RLNPSGA"
     gene            823..1785
                     /locus_tag="VF958_05085"
     CDS             823..1785
                     /locus_tag="VF958_05085"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017953821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HEX9148520.1"
                     /translation="MRIAVFGTGGAGGYFGAQLARSGEEVVFIARGRHLEAIRSNGLV
                     LESPSGEVVVRPAGATDDPQQVGHVDVALVGVKAWQVPEAAASMRPIVGPETFVVPLQ
                     NGVEAPGELADALGEEHVVGGLCGTLSWISGPGRIRTLGKVHFVKLGELDNRPSERTE
                     RLREAFERAGVNAEIPSDIHRALWEKFLFVVSFGGVGSVTRAPVGVVRSIPETRGMLE
                     RCMREILAVGRARGVALADSAVAKSMALVDSLDPSGTTSLQRDIADGKPSELEAWNGA
                     VVRLGREAGVATPTHEVIYQSLLPTELRARGRVEYPAPAAGGQA"
     gene            1804..2109
                     /locus_tag="VF958_05090"
     CDS             1804..2109
                     /locus_tag="VF958_05090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9148521.1"
                     /translation="MEIGILAWLLFGIVSAIIAKKKRRSGCAWFFLGVLLGPFGLILA
                     FAAPSEREPVAPSSQGQATKPRITSPGPAPDYWICPRCGSPNSKKLNRCGNCGYSEE"
     gene            complement(2072..2656)
                     /locus_tag="VF958_05095"
     CDS             complement(2072..2656)
                     /locus_tag="VF958_05095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011524780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HEX9148522.1"
                     /translation="MIGRPGTDEADAYYFRYIDRVPGDDVLGVLEPQLEETVAFLSPI
                     AEERSLHRYGPDKWTMRQVVNHVNDCERLFLLRAFWFARGFDSPLPSFDQHTAADAAR
                     ADAFPWASHVEEFRRIRLATLSFFRNLPAEAWMRRGIASDLPFTVRALAWIAAGHLDH
                     HLAILRERYISSRDSSPPPRGTFTPPSTRSYRSG"
     gene            2776..3162
                     /locus_tag="VF958_05100"
     CDS             2776..3162
                     /locus_tag="VF958_05100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9148523.1"
                     /translation="MLVTGTRRRVAVVVLTVTVLLSVAASGLAQEVIRITSYTARFGK
                     NAEVSKVTDDELTGMYAGMKGLKWVKFYSDPTSGERGSVTVWADRAALDAYMKSDARK
                     GILARLRPLIEGDVSSRVYNVYRPGK"
     gene            3202..3696
                     /locus_tag="VF958_05105"
     CDS             3202..3696
                     /locus_tag="VF958_05105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013630324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HEX9148524.1"
                     /translation="MPSDANRDRALRALLARFLDWEDAHIGFDAAVEGIPARSRGVRP
                     EGAPHSAWQLLEHVRICQWDILEFCRNPEYLELKFEDYWPPTVAPPTAAAWKKSVAAF
                     RRDRDALKELASDPSVDLFAKIPHGTGQTLVRELVLAADHNAYHVGQLVLLRRLLGAW
                     RRTR"
     gene            3693..4652
                     /locus_tag="VF958_05110"
     CDS             3693..4652
                     /locus_tag="VF958_05110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012652722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HEX9148525.1"
                     /translation="MTAQQRLSLARIAAAAAVIDPVFLDSPQFLAESLGRDLDSELLV
                     KVETMNPIRSFKARGTELLTASLDGRPRLVCATAGNFGQGMAWSARKRGLSITVFTAT
                     SANPLKVERMRAFGAEVRQVGRDFDEAHQAAKEFATANGARMVEDGRDVEIAEGAGTI
                     GIELLRWPEPLDSIVVPLGDGALLAGIAHWVKAHRPTTKMIGVCATGAPAMERSWRSG
                     VLCELGEADTIADGIAVTSPFAEALSDITGVTDDVLLVDDGALVAAIRRAHRDLGIVL
                     EPAGAAALAAVLSQPERFRGHRVAAILSGGNATAEQLNRWLLP"
BASE COUNT          770 a         1602 c         1646 g          807 t
ORIGIN      
        1 tgggaggatc cggctgacga caaacgcgtc atcgcctggg cgcgcgactt cttcaatgct
       61 tcagcgccct tcgccagcgg tggcgtctac gtcaatttcc tcaccgccga cgaaggcgat
      121 cgcgtgcgct ccgcgtacgg cccgaactat gatcgcctcg cccaggtgaa gcgcaagtac
      181 gacccgaaaa acctgtttcg cgtgaaccag aacatcgtgc cggcctgaga aaacgaggta
      241 cgccggacga gcgaaggcat tgccagacgc gtcgaagccg atgagtcccg tgtcgcctga
      301 accgccggtc ttctcgaggc ccggcttgat cacggtcctt cccttcctcg tggccgtcgc
      361 ggcgacggct ttcatcgccg gccgctttcg acccggcgcc tggtacgagg cgctcgcgaa
      421 accctcgtgg actcctccgg gctggctctt cgcgccggtg tggttcctcc tctatctggg
      481 catcgccgtc gcgggatgga tcctctggcg tgcagccgga tcgggcgcct ctctccctct
      541 cgcgctctgg gcggcgcagc tcgtcctgaa cgcagcctgg tcctggctgt tcttcggtct
      601 gcaccggccg ggagccgcgc tcgtcgacat cgtccttctt ctcgttctca tcctcgcgtt
      661 cgccctgacg gcccatccct tctctcccgg cgccgcctgg ctgttcgtgc cctacggtct
      721 ctgggttctt ttcgcgacgg ctctcaacac ggcgatcgtg cgactcaacc cgagcggcgc
      781 ctgatccgtt cgtggccaag cggccggaca gataatcctc cgatgcgcat cgcggtcttc
      841 ggcaccggcg gcgccggcgg ttatttcggc gcgcagctcg cgcggtccgg cgaagaggtg
      901 gtcttcatcg cgcgcggaag acatctcgag gcaattcgct cgaacggcct cgtcctcgaa
      961 tcgccctccg gcgaggtcgt cgtcaggccg gccggagcta ccgacgatcc tcaacaggtc
     1021 ggacacgtcg acgtggccct cgtcggagtc aaggcgtggc aggtgcccga ggccgccgcg
     1081 tccatgcgac cgatcgtcgg cccggagacg ttcgtggtcc ctctgcagaa cggagtcgaa
     1141 gcgccgggag agctcgccga cgcactcggc gaagagcacg tcgtcggcgg tctctgcgga
     1201 acgctcagct ggatttccgg acccggccgg atccggaccc tcgggaaggt tcatttcgtg
     1261 aagctgggcg aactcgacaa ccggccgagc gagcgcaccg agcgcctgcg cgaggcgttc
     1321 gagcgggccg gtgtgaacgc ggaaatcccc tccgacatcc accgtgcact gtgggagaag
     1381 ttcctgttcg tcgtgtcgtt cggcggcgtc ggttccgtga cgcgcgcgcc ggtgggcgtc
     1441 gtccgctcga tccccgagac acggggaatg ctcgagcgct gcatgcgcga aatcctcgct
     1501 gtcgggcgcg ctcgcggggt ggcgctggcg gattccgcgg tggcgaaatc gatggcgctc
     1561 gtggactccc tggacccgtc cgggaccacg tctctgcagc gggacatcgc cgacggcaag
     1621 ccgtcggagc tcgaagcctg gaacggcgcc gtcgtccgac tcggacgcga agcgggagtg
     1681 gcgacgccca cccacgaggt catctaccag agcctgctgc cgacagagct gcgggcgcgc
     1741 gggagggtcg agtacccggc gccggcggcc ggcggccagg cgtagcgctg ggagaagatc
     1801 gacatggaaa tcgggatcct cgcctggctg ctcttcggaa tcgtcagcgc gatcatcgcg
     1861 aagaagaagc gaaggagcgg ctgcgcctgg ttcttcctgg gcgtgttgct cggcccgttc
     1921 ggtctgattc tcgcctttgc cgctccgtcc gaacgggagc cggtggcgcc ttcgtcacag
     1981 ggccaggcga cgaagccgcg catcacgtcc ccgggcccgg cgccggatta ctggatatgc
     2041 ccccggtgcg gcagcccgaa ttcgaaaaaa ctcaaccgct gcggtaactg cgggtactcg
     2101 gaggagtgaa agtcccgcgc ggcggaggcg aggagtccct actcgagatg tagcgctcgc
     2161 ggaggatggc gaggtggtgg tcgaggtgac cggcggcgat ccacgcgagg gcgcgcacgg
     2221 tgaaggggag gtcgctcgcg attccgcgcc gcatccacgc ctcggccggg aggttccgga
     2281 agaaagagag cgtcgcaagc cggattctcc ggaactcctc gacgtggctc gcccagggga
     2341 acgcgtcggc ccgcgcggcg tccgcggcgg tgtgctgatc gaagctcggc aacgggctgt
     2401 cgaagcctcg cgcgaaccag aaggcacgga gaaggaagag ccgctcgcag tcgttgacgt
     2461 ggttgaccac ctgacgcatc gtccacttgt ccggtccgta acggtggagc gacctctcct
     2521 ccgcgatcgg cgaaaggaac gcgacagtct cctcgagctg cggctcgagg acgccgagga
     2581 cgtcatcgcc cggaaccctg tcgatgtacc ggaagtagta ggcgtcggct tcgtcggtac
     2641 cgggtcgtcc gatcacgcgc ggaattctag ccgcggcgcg ggcctggtct cgaagccgaa
     2701 ttccaagacc gaattgactc tcttcgcgtg gccgccatag gctccgagag aattccgcct
     2761 ttcgaggagg gaacgatgtt ggtcaccgga actcgtcggc gcgtcgcggt cgtcgtcctc
     2821 acggtgacgg tacttctttc ggttgcggcc tccggcctcg cgcaggaagt catccggatc
     2881 acgagctata cggcccgttt cggcaagaac gccgaagtct cgaaggtcac agacgacgaa
     2941 ctcaccggaa tgtacgccgg gatgaagggc ctcaagtggg tcaagttcta ttccgacccg
     3001 acgagcggcg aacgcggctc ggtgacggtc tgggcggacc gagcggccct ggacgcgtac
     3061 atgaagtccg atgcgcgaaa agggattctc gccaggctgc ggccgctgat cgagggagac
     3121 gtttcgagcc gcgtctacaa cgtctaccgc cccgggaagt aacgggatcc ccgaaatgca
     3181 gctcgggtag agcaccacga aatgccatcc gatgcgaatc gggaccgcgc gctgcgggct
     3241 ctgcttgccc gattcctcga ctgggaggac gcgcacatcg gcttcgacgc ggctgtcgag
     3301 ggaatcccgg cgcgttcccg gggcgtccgc ccggagggcg cgccgcactc ggcctggcaa
     3361 ctgctggagc acgttcgcat ttgccagtgg gacatcctcg agttctgccg caacccggag
     3421 tacctcgagt tgaaattcga agactactgg ccgccgaccg ttgcgccgcc caccgctgcc
     3481 gcctggaaaa agagcgtcgc cgcgttccgc cgcgaccgtg acgcgctgaa agagctggcg
     3541 tcggatcctt cggtcgacct cttcgcgaag atcccgcacg gtaccgggca gacactcgtg
     3601 cgcgaactcg tgctcgccgc cgaccacaac gcctaccacg ttgggcagct cgtcctgctc
     3661 cggcggctgc tcggcgcctg gcgccgaacg cggtgacggc gcagcagcgg ctctcactcg
     3721 cgcggatcgc cgcggcggcg gcggtgatcg acccggtctt cctcgattcg ccccagttcc
     3781 tcgccgagtc gctcggtcgc gatctcgaca gtgagcttct cgtgaaggtc gagacgatga
     3841 acccgatccg gtccttcaag gcccgcggca ccgagctcct gacggcgtcg ttggacggac
     3901 ggccgcgact cgtctgcgcg acggcaggaa acttcgggca ggggatggcg tggtcggctc
     3961 gcaagcgcgg tctttcgatc acggtcttca ccgcgacgag cgccaacccg ctgaaagtgg
     4021 aacgcatgcg cgcgttcggg gccgaggtgc gtcaggtcgg tcgcgacttc gacgaagccc
     4081 atcaggccgc gaaggaattc gcgacggcca acggcgcacg aatggtcgag gatggccgcg
     4141 acgtcgaaat cgccgaaggc gccgggacga tcgggataga actgctgcgt tggccggaac
     4201 cgctcgattc gatcgtcgtc ccgctcggtg acggagcgct gctcgccgga atcgcgcact
     4261 gggtgaaagc acatcgaccg acgacgaaga tgatcggcgt gtgcgcgacc ggcgcgccgg
     4321 cgatggagcg gtcctggcgg tcgggcgtcc tctgcgagct cggggaggcc gatacgattg
     4381 ccgacggaat cgcggtgacg agccccttcg cggaggcgct ctccgatatc acgggcgtca
     4441 cggacgatgt cctcctggtg gacgacggtg cactcgtcgc ggcgatccgt cgcgcccacc
     4501 gggacctcgg catcgtgctg gagcccgcag gcgcggccgc gctcgcggcg gttctgtctc
     4561 aaccggagcg tttccgcggt caccgcgtcg ccgcgatcct ctcgggaggc aacgccaccg
     4621 ccgagcagct gaacaggtgg ctgttgccgt gagcgatttc acccttcgca aggcgactct
     4681 cgaagacagg ccggagctcg agagactcat cgccgactca gcgcgcggtc tctcgcggcc
     4741 ggactacacg gatgcccagg tcgaggcggc gctcggggcc gcgttcgggg tggacagcga
     4801 gctgattcac gacggcacct acttc
//
LOCUS       DASWAV010000250         3177 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U2665
            k141_371895, whole genome shotgun sequence.
ACCESSION   DASWAV010000250 DASWAV010000000
VERSION     DASWAV010000250.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960316
            Sequence Read Archive: SRR10260007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 3177)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3177)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:47:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,929
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3177
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_371895"
                     /isolate="SMAG_U2665"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.36444 N 132.31889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..225)
                     /locus_tag="VF958_05115"
     CDS             complement(<1..225)
                     /locus_tag="VF958_05115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014857298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PtsA"
                     /protein_id="HEX9148526.1"
                     /translation="MSRNRRRKALSALFTGLCGASVALALVPLLFILVYLVQKGLGSL
                     HWTFFVQNPRPVGEAGGGFANAIVGTVILVG"
     gene            complement(222..1169)
                     /gene="pstC"
                     /locus_tag="VF958_05120"
     CDS             complement(222..1169)
                     /gene="pstC"
                     /locus_tag="VF958_05120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011366086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HEX9148527.1"
                     /translation="MRKRDLFRTGTAAPAAIVLLLAVGIAFELVRHSRLSIEKFGLRF
                     WSGKVWDPVSGEFGAFPFLWGTLYSSILALAIAAPISIGIAVFLSELSPRRLRRPLVF
                     LTELLAAIPSIVYGLWGIFVLVPLVRRLETAMPPDLRKLPFFTGPPLGVGMLAAALIL
                     AIMVIPFASAVAREVVRAVPAAQREAAYALGATRFEAIRATLFHGRTGIAGALLLGFG
                     RALGETMAVTMVIGNNPQFSLSLFAPQHTMAAVIANEFTEAADDLYLNALMEIGLVLF
                     VVTLVVNGLSRLLIWGLERKNRPRSEPAAEPVVATAEAA"
     gene            complement(1174..2196)
                     /gene="pstS"
                     /locus_tag="VF958_05125"
     CDS             complement(1174..2196)
                     /gene="pstS"
                     /locus_tag="VF958_05125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006903486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009897 - external side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0042301 - phosphate ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HEX9148528.1"
                     /translation="MRFSRLIAAAALAAAFARPGAAETIRISGAGATFPYPIYSKWFS
                     EYNKLHPDVEINYQSIGSGGGIRQLSNRTVFFGATDGPMTQDQLLSAPGRILHFPTVL
                     GADVPVYNIPGVSEELKFTGPLLADIFLGKVTKWNDPAIGRLNPTVKLPLTDIVVCHR
                     SDGSGTTYIWVDYLSKVSPEWKKTVGVATSVNWPVGVGGKGNEGVAGLVKQTPGAIGY
                     VELIYALQNKIAFGSVQNAAGEFLRASLESVTKAAAATKGKMPPDFRVSITNAPGAGV
                     YPISSFTWILFYENPKDKRAARIMADFMRWALRDGQKYCADLGYAPLPEEVVTMEMEA
                     LKKIRM"
     gene            complement(2257..>3177)
                     /locus_tag="VF958_05130"
     CDS             complement(2257..>3177)
                     /locus_tag="VF958_05130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9148529.1"
                     /translation="ANGDQVHSNAFNVGRAYINVTGQLHHLLSFRITPDITRETGSGS
                     SLSGSYTVRLKYTYAQFNMDDWLPKGTWVRFGLQQTPYVDFNEGVYRYRFQGPIFVDR
                     EGFLTSSDLGLTGRLAFPSNYGDIHLGVYNGEGYTRSETNDQKAFQVRATIRPAPAVP
                     VLRGLRLTGFYDADHYVRDAKRQRAVGFLSFEHPYVNVGVDYLKAKDQTTLAVREIEA
                     EGWSAWVTPRTPIGIEGLFRYDELKPDKDQDPDRKKKRAIAGVAYWFTFLKPAVTAAL
                     LADYENVKYDRFSPARPTEKRYALHMLLNF"
BASE COUNT          593 a          995 c         1063 g          526 t
ORIGIN      
        1 tccgaccagg atgaccgtgc cgacgatcgc gttcgcgaat cctccccccg cctcgccgac
       61 cggccgcggg ttctgcacga agaacgtcca gtggagcgaa ccgagcccct tctgcaccag
      121 gtagacgagg atgaagagga gaggcacgag cgcgagagcg accgacgccc cgcagagtcc
      181 ggtgaagagt gccgagagcg ccttacggcg ccggttgcgg ctcacgcggc ctccgcggtg
      241 gcaacgactg gctcggcggc aggctcgctc cgtggacggt tcttccgctc gaggccccag
      301 atgagcaggc gggagagtcc gttcaccaca agggtgacga cgaagaggac gagcccgatc
      361 tccatcagcg cgttcaggta gaggtcgtcc gcggcctcgg tgaactcgtt ggcgatgacc
      421 gcggccatcg tgtgctgcgg cgcgaagaga gacagggaga actgagggtt gtttccgatg
      481 accatggtga ccgccattgt ttcccccagc gcccggccga agccgaggag gagggcgccc
      541 gcgatcccgg tgcggccgtg gaagagtgtt gcgcggatcg cctcgaatcg agtggcgccg
      601 agcgcgtacg ctgcctcgcg ctgcgccgcc ggcacggcgc ggacgacttc gcgggcgacc
      661 gccgaggcga acgggatcac catgatcgcg aggatcaggg cggcggccag cattcccacg
      721 ccgagcggcg ggccggtgaa gaacgggagc ttgcggagat ccggaggcat ggcggtttcg
      781 agccgacgaa cgagcgggac caggacgaaa atgccccaga ggccgtagac gatcgacggg
      841 atcgcggcga ggagctccgt caggaacacg agcgggcgcc ggaggcgccg gggagagagc
      901 tccgacagaa agacagcgat cccgatcgaa atcggcgccg caatcgcgag cgcgagaatg
      961 gacgagtaga gcgtccccca caggaaagga aacgcgccga actccccgga gaccgggtcc
     1021 cagaccttgc cgctccagaa tcgcaggccg aacttctcga tcgagagtcg cgagtggcgc
     1081 acgagctcga acgcgatgcc gaccgcgagg aggaggacga ttgcggcggg ggccgccgtt
     1141 cccgtgcgaa agaggtctct tttccgcatc cggctacatt cggatcttct tcagagcctc
     1201 catctccatg gtcacgacct cctccggaag cggcgcgtat ccgaggtcgg cgcagtactt
     1261 ctgcccgtcc cgaagcgccc accgcatgaa gtcggccatg atccgcgcgg cgcgtttgtc
     1321 tttcggattc tcgtagaaga ggatccaggt gaaagacgag atcggataaa cacccgcgcc
     1381 gggcgcgttg gtgatggaga cccggaagtc cggaggcatc ttccccttcg tcgccgcggc
     1441 ggccttcgtg accgactcga gcgacgctcg aaggaactct ccggccgcgt tctggaccga
     1501 tccgaacgca atcttgttct ggagggcgta gatgagctcg acatagccga tcgcgcccgg
     1561 agtctgtttg acgaggcccg cgacgccctc gttgcctttc cctcccacac cgaccggcca
     1621 gttcaccgac gtcgccactc cgacggtctt tttccactcc ggcgagacct tcgaaagata
     1681 gtccacccag atgtaagtcg ttcccgagcc gtcggagcgg tggcagacca ctatgtcggt
     1741 cagcgggagc ttgaccgtcg ggttcagtcg gccgatcgca ggatcgttcc atttggtaac
     1801 cttgccgagg aagatgtccg cgaggagcgg gcccgtgaac ttcaactcct cgctgacccc
     1861 ggggatgttg taaaccggca cgtcggcgcc aagcaccgtc gggaagtgaa ggatcctgcc
     1921 cggggccgag agcaactggt cctgcgtcat cggaccgtcc gttgctccga agaacacagt
     1981 gcggttcgag agctgccgga tgccgccgcc cgagccgatc gactggtaat tgatctcgac
     2041 gtccgggtgg agcttgttgt actcggagaa ccacttcgag tagatcggat acggaaacgt
     2101 cgcgccggcg ccgctgatcc ggatcgtttc cgcggcgccg ggtctcgcga acgcggcggc
     2161 cagggccgct gcggcaatca gtctggagaa tcgcatcgtg tcacctcgct cggagtgtta
     2221 agccggcctg taaattcggc atgaagaaga cgtgaactag aagttcaaga gcatgtgaag
     2281 cgcgtagcgc ttctcggtcg ggcgcgcggg cgagaagcgg tcgtacttca cgttttcgta
     2341 gtccgcgagc agcgccgcgg tgacggcagg cttgagaaac gtaaaccagt aggcgacgcc
     2401 ggcgatcgcg cgcttcttct ttcgatccgg atcctggtcc ttgtcgggtt tcagctcgtc
     2461 gtatcggaag agtccctcga tgccgatcgg cgtcctcggg gtcacccagg cggaccagcc
     2521 ctccgcctcg atctcccgga ccgcgagcgt tgtctggtcc ttcgccttga ggtaatcgac
     2581 cccgacgttc acatacggat gctcgaaaga gagaaagccg acggcgcgct gccgcttcgc
     2641 gtcccgaacg tagtggtcgg cgtcgtagaa accggtcagc cgcagcccgc gcagcaccgg
     2701 cactgcggga gccgggcgga tcgtcgcccg gacctgaaat gccttctggt cattcgtctc
     2761 ggaccgggtg taaccctcgc cgttgtagac gcccaggtga atgtcgccgt agttcgacgg
     2821 aaacgcgagg cggcccgtca agccgaggtc cgaggaggtg agaaacccct cccggtccac
     2881 gaagatcggc ccctgaaacc gataccggta gacgccttcg ttgaagtcga cgtagggcgt
     2941 ctgctgcaat ccaaagcgga cccacgtccc cttcggaagc cagtcgtcca tgttgaactg
     3001 cgcgtacgtg tacttcaggc ggaccgtgta actgcccgag agcgagctcc cggagccggt
     3061 ctcgcgcgtg atgtccgggg tgatgcgaaa cgacagcagg tggtggagct ggcccgtaac
     3121 gttgatgtac gcgcggccga cgttgaacgc gttcgaatga acttgatccc cgtttgc
//
LOCUS       DASWAV010000251         5618 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U2665
            k141_454118, whole genome shotgun sequence.
ACCESSION   DASWAV010000251 DASWAV010000000
VERSION     DASWAV010000251.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960316
            Sequence Read Archive: SRR10260007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 5618)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5618)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:47:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,929
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5618
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_454118"
                     /isolate="SMAG_U2665"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.36444 N 132.31889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..209)
                     /locus_tag="VF958_05135"
     CDS             complement(<1..209)
                     /locus_tag="VF958_05135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9148530.1"
                     /translation="MSVAASTPAVERSARGGAPLPIWGAAGVVLFGLGLWALIADLEA
                     WNAIWYLPAWYGYLLVLDAVIFVRTG"
     gene            complement(301..927)
                     /locus_tag="VF958_05140"
     CDS             complement(301..927)
                     /locus_tag="VF958_05140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007065578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diheme cytochrome c-553"
                     /protein_id="HEX9148531.1"
                     /translation="MRRNRVFAMLATAAFVVAVSACRKQEAPGASQPEVVPGAATAAQ
                     PASAPPAAASADLVGGGRYLVTVMGCNDCHTPFKMGKNGPEPDMTRMLSGHPEGLKMP
                     PPPKVDMPWAMVGSATNTAWAGPWGVSYTANLTPDQNTGIGIWTEDMFIRAMRSGKHM
                     GTSRTILPPMPWPWYGKMRDEELKAMFAYLKSIPPIHNRVPDPEPPAR"
     gene            1042..2259
                     /locus_tag="VF958_05145"
     CDS             1042..2259
                     /locus_tag="VF958_05145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014559028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1015 family protein"
                     /protein_id="HEX9148532.1"
                     /translation="MATIRPFPALRPVPQRARDVAAVPYDVVTTEEARALASGNPSSF
                     LHVSRAEIDLPDGTDPYSPDVYRKAAENFERLSRDGALTREREPALYVYRLGMGEHVQ
                     TGIAGAFSIDEYESKVILKHERTRRDKEDDRTRHMMALRAQTGPVFLTYRDRREVDDL
                     VARAVRETPLYDFAAADGISHAIWRLPPGASAGLAREFDRVPFLYIADGHHRAASASR
                     ARDEFRERSDRAGGAAEYDFFLGVAFPASQVRILPYNRLVSDLGGRSADDFLFALKQR
                     VGVSMDAAPAPSRRGEVSMYLAGRWYSLRLSPGPAAEALDVTLLQDAIFAPLLGVRDV
                     RIDKRIEFVGGIRGTSALERAVDERRAAVAFSLFPVAVEDLMKVADANEIMPPKSTWF
                     EPKLRDGLLIHEI"
     gene            2298..3500
                     /locus_tag="VF958_05150"
     CDS             2298..3500
                     /locus_tag="VF958_05150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011685683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX9148533.1"
                     /translation="MKVLVADRFEKSGLDGLAEAGCQVLYEPDAKDDALTEAIRSSGA
                     EVLIVRSTRVTRPMLEVGSLSLVVRAGAGYNTIDVESASERGIFVANCPGKNSTAVAE
                     LAFGLILALDRRIPENVADLKQGVWNKKEYSKAKGLFGRTLGLIGLGGIGCAMCPRAM
                     AFGMPVVGWSRSLTDEKARTLGIERRESIFQVAAASDVVSVHVALKPETTKMIGEEFF
                     DAMRPGSFFVNTSRAEVVDQAALERAVRQKGIRAGLDVFAGEPTGGSGSVDDPIFQLP
                     GVIGTHHIGASTDQAQEAIASETVRIVRNFKETGRAPNVVNLAKKSPATHLLVVRHFD
                     HVGVLAEVFDRIKAAGINVQETENVVFDGAKAAVARIHLNQAPSRDVLAGIRSSTDDI
                     LEVTLVPI"
     gene            3516..4637
                     /gene="serC"
                     /locus_tag="VF958_05155"
     CDS             3516..4637
                     /gene="serC"
                     /locus_tag="VF958_05155"
                     /EC_number="2.6.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018701774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004648 -
                     O-phospho-L-serine:2-oxoglutarate aminotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoserine/phosphohydroxythreonine
                     transaminase"
                     /protein_id="HEX9148534.1"
                     /translation="MATVAEERASKATARVFNFGAGPAVLPEPVLEEAQRDLVSLPGV
                     GMSILEISHRSKTFEEILAAADADIRRLASIPSNYRVLFLQGGASLQFSMVPMNLLPA
                     DRSADYIVTGSWSKKAVKEAQKVGMVSIAASTETEGFSRIPEQSELRLDPEACYVHFT
                     TNNTIFGTEWAREPEVGRVPLVADASSDVFSRPIDVGKYALIYAGAQKNLGPAGVTLV
                     ILREDLLERIPKGLPTMLDYGTHTQNNSLYNTPPVFAIYVMRLVAKWLIAEGGLEEIG
                     RRNSEKAAVLYGALDASGGFYRAHAKPGSRSNMNVTFRLPSEELEKQFLGEAGKNGFS
                     GLKGHRSVGGIRASLYTAFPKKGVDALVSFMKDFAGRNG"
     gene            complement(4641..>5618)
                     /locus_tag="VF958_05160"
     CDS             complement(4641..>5618)
                     /locus_tag="VF958_05160"
                     /inference="COORDINATES: protein motif:HMM:NF012382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HEX9148535.1"
                     /translation="RRAIAKRLDARGLPTKEDEVLVTSGAQQAIGLVATLFLQRGDAV
                     AVESPTYLGAMDLFTNAGAHLVPVPVGEAGVLTGALKDVVSRASPRLAYLVPTFHNPT
                     GSVVPEAARREIARIADESDTPIVEDDTLADLTLGAEPPPPIGAFSRGGTVITVGSLS
                     KVLWGGLRIGWIRASEPVLARLTRLKILTDLGCSILSQLAAVELLEQIETIRRARRRE
                     VRTKYGRMARLLKSLLPSWTWTPPAGGLSLWVRLPHGDATGFAQVALRHGVAVVAGPL
                     ASPDSSGADRLRLPFVHDANTMTEGMQRLARAWRAYSPEARGERTSLIV"
BASE COUNT          932 a         1894 c         1885 g          907 t
ORIGIN      
        1 ccggtgcgga cgaagatcac cgcgtcgagc acgaggaggt atccgtacca ggcgggcaga
       61 taccagatcg cgttccacgc ttcgaggtcc gcgatgagag cccagagtcc gagaccgaag
      121 agaacgaccc ccgccgctcc ccagatcgga agcggggcgc cgccccgcgc cgagcgctcg
      181 acagccggcg tcgaggccgc aacgctcacc tcgagttgct cttggggaac ccgacgtggt
      241 cgggcgcggc gttcggctgc gcgagcagct ttctgatgag gtccgtcgtt tggactcgtc
      301 ctagcgagcc ggcggttccg gatccggcac gcggttatgg atcggcggaa tcgatttcag
      361 gtacgcgaac atcgccttga gctcctcgtc ccgcatcttt ccgtaccacg gccacggcat
      421 cggcggcagg atcgtccggg acgttcccat gtgttttccg gagcgcatcg cgcggatgaa
      481 catgtcctcg gtccagatgc cgatgccggt gttctggtcg ggggtcaggt tcgccgtgta
      541 gctcacgccc cacgggccgg cccacgccgt gttcgtcgcc gacccgacca tcgcccacgg
      601 catgtccacc ttcggcggag gcggcatctt caacccctcg ggatgacccg acagcatccg
      661 ggtcatgtcg ggctcggggc cgttcttccc catcttgaaa ggcgtgtggc agtcgttgca
      721 gcccatgacg gtgacgagat acctcccgcc gccgacgagg tcggccgaag cggccgccgg
      781 cggggcggat gccggttgcg cggccgtcgc cgctcccggg acgacctccg gctgcgacgc
      841 tcccggggcc tcctgcttgc gacaggcgct caccgcgacc acgaaagcag ccgtcgccag
      901 catcgcgaaa actcgatttc gacgcatgag tcgtgctcct tgtcttgaac gtcttgaagc
      961 gaaccccgcc gatcatattg cggaccgcga cagtcggaca cccttgcgca tcgaacccca
     1021 ctagaatcgg ggcgaatcga aatggcgacc atccggccgt tccccgccct tcgacccgtc
     1081 cctcagcgcg cgcgggacgt cgctgccgtt ccctacgacg tcgtcaccac cgaagaggcg
     1141 cgcgccctcg cctcgggcaa cccgtcgagc ttcctccacg tctcgcgcgc cgagatcgac
     1201 cttcccgacg ggacggatcc ctattcgccc gacgtttatc gcaaggccgc ggagaacttc
     1261 gagcggctct cccgcgacgg cgcgctgacg cgagaaaggg agcccgcgct ctacgtctac
     1321 cggctcggca tgggcgagca cgtgcagacg gggatcgcgg gggccttctc catcgacgag
     1381 tacgaatcga aggtgattct caagcacgag cggactcgtc gcgacaagga ggacgatcgg
     1441 acgcggcaca tgatggcgct ccgcgcacag acgggtccgg tgtttctcac ctatcgcgac
     1501 cgccgggagg tcgacgatct cgtcgcccgg gccgtccggg agaccccgct ttacgacttc
     1561 gcggccgcgg acggcatcag tcacgcgatc tggcgcctcc cgccgggagc ctccgcggga
     1621 ctcgcacgcg agttcgatcg ggtgcctttt ctctacatcg ccgacggcca ccaccgggcc
     1681 gcgagcgcca gccgggcgcg ggacgagttc cgcgagcgtt ccgatcgggc cggcggcgcg
     1741 gccgagtacg acttcttcct cggcgtcgca ttccccgcgt cgcaggtccg gatcctcccg
     1801 tacaaccggc tcgtgagtga tctgggcgga cgctccgccg acgactttct cttcgcgttg
     1861 aagcagcggg tcggagtctc gatggacgcg gcgccggcgc cctcgcgtcg aggagaggtg
     1921 agcatgtacc tcgcggggag gtggtacagc ctccgcctct cccccgggcc ggcggcggag
     1981 gcgctcgacg tgacgctcct ccaggacgcg atcttcgcgc cgctcctcgg ggtgcgcgac
     2041 gtgcgcatcg acaagcgcat cgagttcgtc ggcgggatcc gcggaacgtc ggcactcgag
     2101 cgcgccgtcg acgaaaggcg ggcggccgtc gccttctcgc tcttcccggt ggccgtggag
     2161 gacctcatga aggtcgctga tgccaacgag atcatgccgc ccaagtcgac ctggttcgag
     2221 ccgaaacttc gggatggact gctgattcac gagatctgag cgcgttgacc gcgccgcaat
     2281 agaatctccg cctcgtcatg aaggtcctcg tcgctgacag gttcgagaag tccggactcg
     2341 acggcctcgc cgaggccggc tgtcaggtcc tctacgaacc ggacgccaag gacgacgcgc
     2401 tcaccgaggc gattcgctcg agcggcgccg aagtgctcat cgtccgctcg acccgcgtca
     2461 cccgcccgat gctcgaggtc gggagccttt cgctcgtcgt ccgcgcggga gcgggataca
     2521 acacgatcga cgtcgagagc gcatccgagc ggggcatttt cgtcgcgaac tgtcccggga
     2581 agaactcgac cgcagtcgcc gagctcgcat tcgggctgat cctcgcgctc gaccggcgca
     2641 tccccgagaa cgtggcggat ttgaaacagg gagtctggaa caagaaggag tacagcaagg
     2701 cgaagggact cttcgggcgc acgctcggac tgatcgggct cggcggcatc ggttgcgcga
     2761 tgtgtcctcg ggcgatggcg ttcggcatgc cggtggtggg gtggagccga agcctgaccg
     2821 acgagaaggc gcgcacgctc gggatcgaac gacgtgagtc gatcttccag gtcgccgcgg
     2881 cgtccgacgt cgtgagcgtc cacgtcgcgt tgaagccgga gacgacgaag atgatcggcg
     2941 aggaattctt cgacgcgatg cggccgggct cgttcttcgt gaacacgtcg cgcgcggaag
     3001 tcgtcgacca ggccgcgctc gagcgcgccg tgcggcagaa ggggatccgc gccggattgg
     3061 acgtcttcgc gggcgagccg accggtggga gcggatcggt cgacgacccg atcttccagc
     3121 tccccggcgt catcgggacc catcacatcg gcgcctctac cgatcaggcg caggaggcaa
     3181 ttgcctccga gaccgttcgc atcgttcgca atttcaagga gaccggtcgc gcgcccaacg
     3241 tcgtcaatct cgcgaagaag tcgcccgcca cccacctgct ggtcgtgcgg cacttcgatc
     3301 acgtcggcgt cctggcagag gtcttcgacc ggatcaaggc ggccggaatc aacgtccagg
     3361 agacggaaaa tgtcgtcttc gacggcgcga aagccgccgt cgcgcgaatc cacctgaacc
     3421 aggcgccctc cagggacgtc ctcgccggca tccggtcgtc gacggacgac atcctcgagg
     3481 tcacgctggt tccgatctag acgaggagtg tgaccatggc caccgtcgcg gaagaaagag
     3541 cctcaaaagc gactgcccgt gttttcaact tcggcgccgg cccggcggtc ctgcccgagc
     3601 cggtcctcga agaggcgcag cgcgacctcg tgtcgctgcc cggcgtcggg atgtcgattc
     3661 tcgagatcag tcatcgctcg aaaaccttcg aagagatcct cgcagccgcc gacgcggaca
     3721 ttcggcgtct cgcgtcgatc ccgtcgaact accgcgtgct ctttctccag ggcggcgctt
     3781 cgcttcagtt ctcgatggtt ccgatgaacc tgctccccgc cgaccggagc gccgattaca
     3841 tcgtcaccgg ctcctggtcg aagaaggcgg tcaaggaggc gcagaaggtc ggaatggtgt
     3901 cgatcgccgc ctcgaccgag acggaggggt tctcgcggat cccggagcag tccgagctgc
     3961 ggctcgaccc cgaggcctgc tacgtccact tcacgacgaa taacacgatc ttcgggacgg
     4021 agtgggcccg cgagccggag gtcgggaggg taccgctcgt cgcggacgcg tcctcggacg
     4081 tcttcagccg ccccatcgac gtcggcaagt acgcgctgat ctacgcgggc gcgcagaaaa
     4141 acctgggacc ggcgggcgtg accctcgtta tcctgcgcga ggatcttctc gagcggatcc
     4201 cgaagggtct gccgacgatg ctcgattacg gcacgcacac gcagaacaac tcgctttaca
     4261 acactccgcc ggtgttcgcg atctacgtca tgcggctcgt cgcgaaatgg ctgatcgcgg
     4321 agggcggcct cgaagagatc gggcgccgga attccgagaa ggcggcagtt ctttacggcg
     4381 cactcgacgc ttccggcgga ttctaccgag cccatgcgaa gccgggcagc cggtccaaca
     4441 tgaacgtgac gttccgcctg ccctccgagg aactcgagaa gcagtttctc ggcgaggccg
     4501 ggaagaacgg gttttcggga ctgaaaggcc atcgctcggt cggcggcatc cgggcgtccc
     4561 tctacaccgc gtttccgaag aaaggcgtcg acgcgctcgt ctcgttcatg aaggacttcg
     4621 ccggccggaa cgggtagaaa ctagacgatc aggctcgtcc gctccccccg ggcctccggc
     4681 gaataggcgc gccacgcgcg ggcgaggcgc tgcatcccct cggtcatcgt gttcgcgtcg
     4741 tgcacgaagg gaagcctcag tcgatcggcg ccactgctat ccggcgaggc gagcgggcca
     4801 gccacgaccg cgacgccgtg ccggagggcc acctgcgcga atccggtcgc gtcgccgtgc
     4861 gggaggcgca cccagagcga gaggccgccc gcgggaggcg tccaggtcca ggacggcagc
     4921 aggctcttca ggagccgcgc catgcggccg tacttcgtcc ggacttcgcg gcgtcgcgcc
     4981 cggcgaatcg tttcaatctg ctcgagcagc tcgaccgcgg cgagctggga gaggatcgag
     5041 cacccgagat cggtcaggat cttcagtcgc gtgagacgcg cgaggaccgg ctcggacgcg
     5101 cggatccatc cgatccgcag gcctccccag aggaccttcg agagggaccc gacggtgatg
     5161 acggttcctc cgcgagagaa cgccccgatc ggcgggggcg gttcggcccc gagcgtcagg
     5221 tcggcaagtg tgtcgtcctc gacgatcggt gtatcggact cgtcggcgat ccgagcgatc
     5281 tcccgccgcg cggcttcggg aacgaccgag ccggtcgggt tgtggaaggt gggtacgagg
     5341 taggcgaggc gaggggacgc gcgggagacg acgtctttga gcgccccggt caagacgccc
     5401 gcctcgccga ccggcacggg tacgaggtgc gcgccggcgt tcgtgaagag gtccatcgct
     5461 ccgagatacg tcggcgactc gaccgcaacc gcgtcgcctc tttgaaggaa aagcgtggcg
     5521 acgagtccga tggcctgctg cgcgccgctc gtgacgagga cttcgtcttc cttcgtcgga
     5581 agaccgcgcg cgtccaaccg cttcgcgatt gcccgccg
//
LOCUS       DASWAV010000252         1503 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U2665
            k141_454131, whole genome shotgun sequence.
ACCESSION   DASWAV010000252 DASWAV010000000
VERSION     DASWAV010000252.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960316
            Sequence Read Archive: SRR10260007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 1503)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1503)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:47:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,929
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1503
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_454131"
                     /isolate="SMAG_U2665"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.36444 N 132.31889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..587)
                     /locus_tag="VF958_05165"
     CDS             complement(<1..587)
                     /locus_tag="VF958_05165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006000392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00282 family metallophosphoesterase"
                     /protein_id="HEX9148536.1"
                     /translation="MNILFIGDVVGSPGRRALERGLLPLIDRRKVDFTIVNVENAAGG
                     FGLTVELYGELSKLPIDVFSSGNHIWDKKEIYEVLNSSDRLLRPANYPPGNPGRGVTV
                     QQTAAGVPVAVLNLQGQVFMPAQCDSPFRVADEELARLSDVKVIVVDMHGEATSEKVA
                     IGWYLDGRVSAVLGTHTHVATADETLRPGGTAYQT"
     gene            complement(603..>1503)
                     /gene="rny"
                     /locus_tag="VF958_05170"
     CDS             complement(603..>1503)
                     /gene="rny"
                     /locus_tag="VF958_05170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - RNA endonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HEX9148537.1"
                     /translation="KGRIIGREGRNIRALEAATGVDLIIDDTPEAIVISAFEPIRREV
                     ARLALEKLIVDGRIHPARIEEIVEKVRSELWEKIRQEGEATALEFSIPDIHPELIKLL
                     GRLKYRTSYGQNVLQHSKEVAWLASSMAAELGAKMEIAKRAGLLHDIGKAIDREMEGT
                     HLELGREVLKKFGETDEVIHAMECHHGDYEPRSLEAVLVNGADALSAARPGARREILE
                     TYVKRLEKLEHIADGFKGVQKAFAIQAGRELRIIVEANRVTDDEALWLSRDIAKKIEA
                     ELQYPGQIKVTVIRETRSVEYAR"
BASE COUNT          227 a          501 c          490 g          285 t
ORIGIN      
        1 tcggtctggt acgcggtgcc cccgggacgg agggtctcgt cggcggtcgc gacgtgggtg
       61 tgcgttccca ggaccgccga aacgcgcccg tcgagatacc aaccgattgc gaccttctcg
      121 gaagtggcct cgccgtgcat gtcgaccacg atcactttga cgtcggagag acgcgcgagc
      181 tcctcgtccg cgacgcgaaa gggcgaatcg cactgcgccg gcatgaagac ctggccctgg
      241 aggttcagca ctgcgaccgg aacgccggcc gcggtctgct gaacggtcac gccgcgcccc
      301 gggttgcccg gcggatagtt cgccggccgc agcagccgat cggaagagtt cagaacctcg
      361 tagatctcct tcttgtccca gatgtggttg cccgaggaga agacgtcgat cggcagcttc
      421 gagagctctc cgtagagctc gactgtcaac ccgaagccgc ctgcggcgtt ctcgacgttg
      481 acgatcgtga agtccacttt gcgccggtcg atgagcggca ggaggccgcg ctcgagcgcg
      541 cgccggccgg gagagccgac cacgtcgccg atgaagagga tgttcatggg gcggaatccg
      601 acttagcgcg cgtactcgac ggaccgcgtc tcacggatga cggtgacctt gatctgtccg
      661 ggatattgca gttccgcctc gatcttcttc gcgatatcgc gtgagagcca caaggcctcg
      721 tcatcggtga cgcggttcgc ttccacgatg atgcgcagct cccgccccgc ctggatcgcg
      781 aacgcctttt gtactccctt gaagccgtcg gcgatgtgct cgagcttctc gagccgcttg
      841 acgtaagttt cgaggatctc gcgccgggcg cccggccgcg ccgccgagag cgcgtcggcg
      901 ccgttgacga gcaccgcctc gagcgaccgc ggctcgtagt cgccgtggtg gcactccatg
      961 gcgtggatca cctcgtcggt ttcgccgaac ttcttcagca cctcgcggcc cagctcgaga
     1021 tgagtgccct ccatctcgcg gtcgatcgcc ttcccgatgt cgtggaggag ccccgcgcgc
     1081 ttggcgatct ccatcttcgc ccccagctcc gcggccatgc tcgaagcgag ccaggcgacc
     1141 tccttggagt gctggaggac gttctggccg taggaggtcc ggtacttcag ccggcccagc
     1201 agcttgatga gctccgggtg gatgtcgggg atcgagaatt cgagcgcggt cgcttcccct
     1261 tcctgccgga tcttctccca gagctccgat cggaccttct ccacgatctc ctcgatacgg
     1321 gccgggtgga tacgtccgtc tacgatcagc ttttcgagcg cgagccgggc gacctcgcga
     1381 cggatcggct cgaacgcgct gatgacgatc gcctcgggag tgtcgtcgat gatgaggtcc
     1441 acgccggtcg cggcctcgag cgcccggatg ttgcgtcctt cgcgcccgat gatgcggccc
     1501 ttc
//
LOCUS       DASWAV010000253         1955 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U2665
            k141_613724, whole genome shotgun sequence.
ACCESSION   DASWAV010000253 DASWAV010000000
VERSION     DASWAV010000253.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960316
            Sequence Read Archive: SRR10260007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 1955)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1955)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:47:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,929
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1955
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_613724"
                     /isolate="SMAG_U2665"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.36444 N 132.31889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1955
                     /locus_tag="VF958_05175"
     CDS             <1..>1955
                     /locus_tag="VF958_05175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014067884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HEX9148538.1"
                     /translation="KNPDLVYVAALGHVWGPNSERGIYRSRDAGKSWEKVLFVSDKTG
                     ASDLVMDPTNPRILYAGFWQVYRKPWALVSGGTEGGIWKTADGGDTWKKLSEGLPDGI
                     VGKVGVAVSPARPDRVWALVESKDKGGVYRSDDGGEKWTRVNSENKLRQRAWYYTRIY
                     ADPKNPDGVYALNTGFYRSNDGGKTFNSIRVPHGDNHDLWIDPDDPQRMIESNDGGAN
                     VSFNGGRTWSSIMNQPTAQFYRVTTDDRFPYWVYGAQQDNTTVAISSRGRGRGIDVTD
                     WHAVGGCESGWVAPKPKNPDVVFAGCYGGSITRYDHKTGQEREVMAWPQLAIGQAAKD
                     LKYRIQWNAPIVISPHDPNVLYQGSQVLLRSRDEGQLWEEASPDLTRNDKSKQDYSGG
                     PITRDNTGIEVYDVIFTIVESPHEAGTIWVGTDDGLVQLTRDGGKNWQNVTPKGIPEW
                     IQINSLEVSPHDKATAYVAATMYKFDDFRPYLYKTNDYGKTWTRIDAGIPAGAFTRVV
                     REDPSRRGLLYAGTETGLYVSFDDGEAWQPMQLNLPVVPITDLAVKEKDLVVATQGRA
                     FWILDDLTPVQEYKPSIAGERVHLFPPRPAVRMDAGGFEGEGSSGALGKNPPNGVVLS
                     YWLKEKPGEKDVLTIEILEDNTVLRRY"
BASE COUNT          397 a          617 c          655 g          286 t
ORIGIN      
        1 gaagaacccg gacctcgtct acgtcgcggc gctcgggcac gtctggggcc cgaattccga
       61 gaggggcatc taccgatcga gagacgccgg gaagagctgg gagaaggtcc tcttcgtcag
      121 cgacaagacg ggcgcctccg atctcgtcat ggatcccacc aacccgcgga tcctctacgc
      181 cgggttctgg caggtgtacc gcaagccttg ggcgctcgtg tcgggaggca cggaaggcgg
      241 catctggaag accgccgacg gcggcgacac gtggaagaag ctgtccgaag gtctcccgga
      301 cggcatcgtc gggaaagtgg gcgtggcggt ctctccggcg cgcccggacc gcgtgtgggc
      361 gctcgtcgag tcgaaagaca agggaggcgt gtaccgatcg gacgacggcg gggagaagtg
      421 gactcgcgtc aactcggaga acaagctccg gcagcgcgcc tggtactaca cgcgaatcta
      481 cgccgacccg aagaaccccg acggcgtgta cgcgttgaac accggcttct accgctcgaa
      541 cgacggcgga aagaccttca actcgatccg cgtcccccac ggcgacaacc acgacctctg
      601 gatcgatccg gacgatccgc agcggatgat cgagtcgaac gacggcggcg ccaacgtgtc
      661 gttcaacggc ggccgaacct ggtcttcgat catgaaccag ccgacggcgc agttctaccg
      721 cgtgaccacc gacgaccgct tcccgtactg ggtctacggg gcacagcagg acaacacgac
      781 cgtggcgatc tcgtcacgcg gccgcgggcg gggaatcgac gtcaccgact ggcatgccgt
      841 cggcggctgc gaaagcggat gggttgcccc gaagccgaag aatccggacg tcgtcttcgc
      901 cggctgctac ggcggttcga tcacgcgcta cgaccacaag accggccagg agcgcgaggt
      961 catggcctgg ccgcagctcg cgatcgggca ggcggcgaag gacctgaagt accggatcca
     1021 gtggaacgcg ccgatcgtca tctcaccgca tgaccccaac gtgctgtatc aggggtcgca
     1081 ggtccttctg cgtagccgcg acgaggggca gttgtgggag gaagcgagcc cggacctgac
     1141 ccgcaacgac aagtcgaaac aggactactc cggcgggccg atcacgcgcg acaacacggg
     1201 catcgaggtc tacgacgtca tcttcacgat cgtcgagtct cctcacgagg cgggcacgat
     1261 ctgggtggga accgacgacg ggctcgttca gctcacgcgc gacggcggaa agaactggca
     1321 gaacgtgacg ccgaaaggaa tccccgagtg gatccagatc aactcgctcg aggtgtcacc
     1381 ccacgacaag gcgaccgcct acgtcgcggc cacgatgtac aagttcgacg acttccgccc
     1441 gtacctctat aagacgaacg actatgggaa gacgtggacg aggatcgacg ccgggatccc
     1501 cgcgggagcc ttcacgcgcg tcgtgcgcga agacccgtcg cggcggggac tgctctacgc
     1561 cggaacggag acgggtctgt acgtctcgtt cgacgacggc gaagcgtggc agccgatgca
     1621 gctgaacctt cccgtcgttc cgatcaccga cctcgcggtg aaagagaagg acctcgtggt
     1681 cgcgacgcag gggagggcct tctggatcct cgacgatctc acgccggtcc aagagtacaa
     1741 gccttcaata gcgggcgagc gcgtgcacct ctttcctccg cgccccgcgg tgcgtatgga
     1801 cgcgggagga ttcgaggggg agggttcctc cggcgcgctg ggcaagaacc cgccgaacgg
     1861 ggtcgtcctt tcgtactggc tgaaggagaa gccgggcgag aaggacgtcc tgacgatcga
     1921 gatcctcgaa gacaacactg tgcttcgaag gtaca
//
LOCUS       DASWAV010000254         3932 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U2665
            k141_571093, whole genome shotgun sequence.
ACCESSION   DASWAV010000254 DASWAV010000000
VERSION     DASWAV010000254.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960316
            Sequence Read Archive: SRR10260007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 3932)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3932)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:47:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,929
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3932
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_571093"
                     /isolate="SMAG_U2665"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.36444 N 132.31889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            134..994
                     /locus_tag="VF958_05180"
     CDS             134..994
                     /locus_tag="VF958_05180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9148539.1"
                     /translation="MRRGILPAALVSLLAAAPLLAHPSVSVVMDSRGTVFYSDLARVW
                     RISRDGHKSVAVDGVHTHELWMDPQGSLYGEHLWYEGDATKQWGYRVWRRSPDGTVTD
                     VRPARRGFRSDYSFVRDAGGASYWVERESKPPRFRKRSADGVVSTLAECASCLDVRWM
                     TAASDGTLYFVDGRDLYEISRAGAVRLRARGLGRSTLLRPQAGGRHVVMGLWTDAARN
                     VYAAVYGTGEVKRIAPDGRVTVVTRSPLPWSPSGGMVGPDGSLWILEYSITNAVRLRR
                     IDRTGRVTVF"
     gene            1013..1468
                     /locus_tag="VF958_05185"
     CDS             1013..1468
                     /locus_tag="VF958_05185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9148540.1"
                     /translation="MNTPVAMRVGFWFALMLSVALAGAFALTAAGFLPYRIAGETVSR
                     EAWWRIAPVLPVVSALAAAVAFGIRRRRRWVRHLVILVWVTLAAAAVISGVRGDISRS
                     VVIRALIEPALLAVLCGWYFYFKPNVVDYFRALKPGGAEATISGMRSQE"
     gene            1465..1938
                     /gene="msrA"
                     /locus_tag="VF958_05190"
     CDS             1465..1938
                     /gene="msrA"
                     /locus_tag="VF958_05190"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020717781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HEX9148541.1"
                     /translation="MNQKATFAAGCFWGVEAAFRQVPGVVDAAVGYTGGKLPNPTYED
                     VCTDRTGHAEAVQVEFDPAKVSYDRLLDVFWENHDPTTKNRQGPDVGTQYRSAIFYHD
                     AEQERAAKDSIVHQEKSGRFRRPIVTEIVPAAEFWRAEEYHQRYLEKRGLAHCKI"
     gene            1946..2545
                     /locus_tag="VF958_05195"
     CDS             1946..2545
                     /locus_tag="VF958_05195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014649460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1572 family protein"
                     /protein_id="HEX9148542.1"
                     /translation="MASGDLTPNRTLGRHYLEETTAEFRKYKKFADKAVAQVSDADFF
                     RQIDPESNSVALLMKHIAGNLRSRWTDFLTSDGEKPDRNRDNEFERYDSDTKESILAR
                     WESGWRTLFEALAPLGDDDLQRTVAIRGEPHTVVQAINRQLTHYASHVGQIVFLAKHL
                     AGDRWKTLSIPRGKSREFEVSRKGAPYRADADTDSRRIR"
     gene            2542..2841
                     /locus_tag="VF958_05200"
     CDS             2542..2841
                     /locus_tag="VF958_05200"
                     /inference="COORDINATES: protein motif:HMM:NF019590.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NIPSNAP family protein"
                     /protein_id="HEX9148543.1"
                     /translation="MIVVRNVFRLKFGKAKDALALWKEGRQIAQRVGQSGSMRAMTDL
                     VGPFYTLVMEEEYSSLADMEQELKSEIGADEWKVWYQKFVPLVESGYREVFTLVE"
     gene            2854..3702
                     /locus_tag="VF958_05205"
     CDS             2854..3702
                     /locus_tag="VF958_05205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015189442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MOSC N-terminal beta barrel domain-containing
                     protein"
                     /protein_id="HEX9148544.1"
                     /translation="MAAAAAGSVRSLWRYPVKSMLGEELDSCDVTERGFVGDRAYALV
                     DDEDGKVASAKNPRKWAKLFECRARFSDDGGEVRITLPGGATVSARDPEAAELLSGLL
                     GRKTTLRTSAAGPPVIEELWLDGAPDGRAVTDEIIARGSPAGTFFDYSVVHLVTTGTL
                     ARLSELSPAGRFDVRRFRPNLVVSTADGAAEFVENAWVGRTVTIGGSVRLAVTDPCPR
                     CVMATLAQEDLPADPGILRTAARHNSVVGGEGRGPQGAYAASVGVYARVLAAGTVRRG
                     DPVRIL"
BASE COUNT          653 a         1235 c         1383 g          661 t
ORIGIN      
        1 aggatcagct cgtctaccac gccgcggtcg gctcggcgga gaacgtcgag gacctgcgcg
       61 agcggtttcc cgaccgcgcg ttacgggagg tccttccgca gtcggtcgcg atctggcggc
      121 ggaggggaaa gagatgagga gggggattct acctgcggcc ctcgtgtcgc tcctcgccgc
      181 tgcaccgctc ctcgctcatc cgagcgtgag cgtcgtcatg gactctcgcg gcaccgtctt
      241 ttattcggac cttgcgcgcg tgtggaggat ttcgcgcgac gggcacaagt cagttgcggt
      301 cgacggcgtt cacacgcacg agctctggat ggacccgcag gggagtctct acggcgagca
      361 cctctggtac gagggggacg ccacgaagca gtggggatac cgcgtgtggc gccggagtcc
      421 cgacgggacc gtgacggacg tgcgccccgc gcgaagaggg tttcgctccg actacagctt
      481 cgtgcgcgac gccggaggcg cctcgtactg ggtcgagcgc gaaagcaaac cgccgcggtt
      541 tcgaaagcga agcgcggacg gcgtcgtgtc gacgctggcc gagtgcgcct cgtgcctcga
      601 cgtgcgctgg atgacggcgg cgtccgacgg cactctctat ttcgtggacg gtcgggacct
      661 ctacgagatc tcgcgggccg gggcggtccg gttgcgcgcg cggggactcg gccgctcgac
      721 gttgctgcgc ccgcaggcgg gcggtcgcca cgtggtgatg ggactgtgga cggacgcggc
      781 gcggaacgtc tacgcggcgg tgtacggcac cggcgaagtg aagcgcatcg cgccggacgg
      841 gcgtgtcaca gtcgtcacgc ggtcgccgct cccatggtcg ccgagcggcg ggatggtcgg
      901 gccggacggc agcctgtgga tcctcgagta ctcgatcacc aacgccgtgc gtctccgtcg
      961 catcgatcga accggtcgtg tcaccgtctt ctagcgtgcg ttgttctcga ggatgaacac
     1021 gcccgtcgcg atgcgcgtcg gcttctggtt tgcgctcatg ctatcggtcg ctctcgccgg
     1081 cgccttcgct ctgacggccg cgggctttct tccgtaccgg atcgccggcg agacggtatc
     1141 gcgggaggcg tggtggcgca tcgcgccggt actcccggtc gtgtccgccc tcgctgcggc
     1201 cgtggcattc ggcatccgcc ggcggcgccg gtgggtgcga cacctcgtca tccttgtgtg
     1261 ggtgacgctc gcggccgcag cagtaatcag cggagtccgg ggcgatatct cgcggtccgt
     1321 cgtgatccgc gcgctcatcg agccggccct gctggcggtc ctctgcgggt ggtatttcta
     1381 tttcaaaccg aacgtggtgg actacttccg cgcgctgaaa ccgggcggcg ccgaagcgac
     1441 tatttccggg atgaggagcc aggaatgaac cagaaagcga cgtttgcggc cgggtgtttc
     1501 tggggggtcg aggcggcgtt ccggcaggtg ccgggcgtgg tcgacgccgc cgtcgggtac
     1561 acggggggca aactcccgaa cccgacctac gaggatgtct gtaccgatcg cactggtcat
     1621 gccgaggcgg tgcaggtcga gttcgatccg gcgaaagtgt cctacgaccg gctcctcgac
     1681 gtcttctggg agaaccacga cccgacgacg aaaaaccgcc agggcccgga cgtcggaacg
     1741 cagtaccgct cggcgatttt ttaccacgac gccgaacagg agagggcggc gaaagactca
     1801 atcgttcatc aggagaagag cggccgcttc cgccgtccga tcgtcacgga gatcgttccg
     1861 gcggcggagt tctggcgggc ggaggagtat caccagcggt acctggagaa gcgcgggctc
     1921 gcgcactgca agatctgagg tcaatatggc aagtggagac ctgaccccca accggaccct
     1981 cggccggcac tacctcgaag aaacgaccgc ggagtttcgc aagtacaaga agttcgcgga
     2041 caaggcggtc gcccaggtct cggacgccga cttcttccgg cagatcgatc ccgagtcgaa
     2101 ctcggtcgcg ctcctgatga agcacatcgc gggcaacctc cgctcgaggt ggacggattt
     2161 cctgacgagc gacggcgaga agccggaccg caaccgcgac aacgagttcg agcggtacga
     2221 ctcggacacg aaggagagca tcctcgcgcg gtgggaatcg ggctggcgga cgctcttcga
     2281 ggcgctggcg ccgctcggcg acgacgatct tcagcgcact gtggccatcc ggggggagcc
     2341 gcacaccgtc gtccaggcga tcaaccggca gctcacgcac tacgcgtcgc acgtgggcca
     2401 gatcgtcttt ctcgcgaagc atctggccgg cgatcgctgg aagactctct cgatcccgcg
     2461 cggaaagtcg cgggagttcg aggtcagcag gaagggagcg ccctatcggg ctgacgcaga
     2521 cacagattca aggaggattc gatgatcgtg gttcgcaacg tattccgttt gaagttcgga
     2581 aaggcgaagg acgcccttgc gctctggaag gaggggcggc aaatcgccca gagagtcggc
     2641 cagagcggat cgatgcgtgc gatgaccgac ctcgtcgggc ccttctatac cctcgtcatg
     2701 gaagaggagt actcgtcgct cgccgacatg gagcaggagt tgaaaagcga gatcggcgcc
     2761 gacgagtgga aggtctggta ccagaagttc gtcccgctcg tcgaaagcgg ataccgcgaa
     2821 gtcttcaccc tggtggagta gaggcgaatg gggatggccg cggcggcggc aggatcggtg
     2881 cggtctctct ggcggtatcc cgtcaagtcc atgctcggcg aagagctcga ctcatgtgac
     2941 gtgacagaac ggggtttcgt gggggaccgg gcctacgcgc tcgtcgacga cgaagacggc
     3001 aaagtcgcga gcgcgaagaa cccgcgcaag tgggcgaagc tcttcgagtg tcgcgcgagg
     3061 ttttccgatg acgggggcga ggtgcggatc acgctgccgg gcggcgcgac ggtctccgcc
     3121 cgcgatccag aggccgcgga gctcctctca ggcctcctcg ggcggaagac gacgctccgc
     3181 acgtcggccg cgggtccgcc ggtcatcgag gagctatggc tcgacggcgc tcccgatggc
     3241 cgcgcggtga cggacgagat catcgccagg ggatctccgg ccggaacgtt cttcgattac
     3301 tccgtcgtcc acctcgtcac gaccgggacg ctcgcccggc tttcggagct ctctcccgcc
     3361 ggacgcttcg acgtccgacg cttccggccg aacctggtcg tctccaccgc cgacggggcg
     3421 gcggaattcg tcgaaaacgc ctgggtcgga aggacggtga cgatcggcgg ctcggtgcgt
     3481 ctcgccgtca ccgatccctg cccgcgctgc gtgatggcga cgctggcgca ggaggatctg
     3541 ccggcggacc ccgggatcct gcggaccgcc gcgcgtcaca attcggtcgt cgggggcgag
     3601 gggcgcgggc cccagggcgc gtacgcggca tcggtcggcg tctacgcgcg cgtcctcgcc
     3661 gccggcaccg tgcggcgggg ggatcccgtc cggattctct agtgtaagtt attcccggtc
     3721 aatggcttgc cttcgaatgt ggcaagtgga gacctgaccc ccaatgtggc aagtggagac
     3781 ctgaccctct tcactacaaa agttgattta cgcctttatt acgcctagag tcggaggatg
     3841 ccggtctcga agccgccccg gttcgacacc cctcaccccg accctctccc cgcccgcggg
     3901 gagagggaga aatacgagaa caccgacgtc cg
//
LOCUS       DASWAV010000255         2846 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U2665
            k141_293504, whole genome shotgun sequence.
ACCESSION   DASWAV010000255 DASWAV010000000
VERSION     DASWAV010000255.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960316
            Sequence Read Archive: SRR10260007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2846)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2846)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:47:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,929
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2846
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_293504"
                     /isolate="SMAG_U2665"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.36444 N 132.31889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..843)
                     /locus_tag="VF958_05210"
     CDS             complement(<1..843)
                     /locus_tag="VF958_05210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9148545.1"
                     /translation="MTRRAVTFLPYAVFLLLAAVSWNRWIEPYVDTGRELMVPWRVAH
                     GDVLYRDVRFYYGPLAPWLAAAVDLAAGRSFAARIALAAAVALLHLEALRRLARRMLS
                     PSRAALATALVVAVCFFLRPGGCHLFPYSLDTSLAVASATGALLLASSSGSRSRDAGV
                     ALLLLAAVLARPELGLAGIGALTLERLVGGDSAAKRRLLLIAAAPVGAAAAIYAAISA
                     GTPLPILKKEGWLTFLFLPPEFRSVYTSFAGVDRPGLRGAELALAAIALLAIGLWLLL
                     AAIVA"
     gene            complement(840..1358)
                     /locus_tag="VF958_05215"
     CDS             complement(840..1358)
                     /locus_tag="VF958_05215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9148546.1"
                     /translation="MKISAIAAGAIAAALALASFVSFRAALSTKIDRVASLESAVVRP
                     AAAARLAAELAVHRLARVSEGESRAVAGAFRSAEGNAALEGYRKAGGSAALLEEATKA
                     LAAEGTDAAADVAFLRAIRGLRAEAAETPVPARPELPSSARRAATFLLAASAAAVAAG
                     FLAYAAGRSRAP"
     gene            1375..2409
                     /locus_tag="VF958_05220"
     CDS             1375..2409
                     /locus_tag="VF958_05220"
                     /inference="COORDINATES: protein motif:HMM:NF015251.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HEX9148547.1"
                     /translation="MTASAGISERSGDLPGYDSPPMQEKMASRNPVVRAIASATSHVW
                     FPLSLFLARRLPRRLLQALLRQTASRFMQIRPKYQRALRANLSIILGRDPASRQVRHA
                     AKGLLDAHFTAWLDFLHFATRPPEEAARLVESVVGYSHIVEGRAKGNGVLLLTAHLGN
                     WEVGGLMLAEMREPVYVVLVPDMFPAVERARRALHERAGVREIRVDRSFGPTLAVLRA
                     LKENAIVAMQGDRDFSNTGVAVPFFGRDAYFPRGPFLVAMATAATVLPAFIVRVPDGR
                     YRAIVEAPLEIDRGRDREAALAANVRRYVAILERYVAGHPEQWYCFYPFWEDPSRGRR
                     ADTAATKNAS"
     gene            complement(2340..>2846)
                     /locus_tag="VF958_05225"
     CDS             complement(2340..>2846)
                     /locus_tag="VF958_05225"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF019846.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX9148548.1"
                     /translation="IVPTFLRRAERNVRSRFPLLPAEFSRLDMNRDFSEQGIEPGSAD
                     VLYAVNTIHIAKDLAATLAQVRATLKPGGVAVFSECVRPFEGQPIYVEFVFNFLENFT
                     GVATDAATRPNHGFLTPESWRRSLAAAGFEKPEILPDVENLARHYDAFFVAAVSARRP
                     RLGSSQNG"
BASE COUNT          461 a          981 c         1031 g          373 t
ORIGIN      
        1 ggcgacgatc gccgcgagca ggagccagag gccgatcgcg agaagcgcga tcgcggcaag
       61 ggcgagctcg gcgccgcgca ggcccggccg gtcgacgccg gcgaacgacg tgtacacgct
      121 tcgaaactcg ggcggaagga aaaggaacgt gagccacccc tccttcttca ggatcgggag
      181 cggcgtgccg gccgagatcg ccgcgtagat cgcggcggcc gctccgaccg gtgccgcggc
      241 gatgagaagg agccgccgct tcgccgctga gtcgccgccg acgagccgct cgagcgtgag
      301 cgcgccgatt cccgcgagcc cgagctcggg gcgtgcgagc accgctgcca ggagcaggag
      361 ggcgacgccg gcgtcccggg atcgggaccc gctagaggag gcgagaagaa gcgcgccggt
      421 cgcggaggcg accgcgagcg aggtgtcgag gctgtagggg aagagatggc agccgcccgg
      481 tctcaggaag aagcagaccg ccacgacgag cgcggtcgcg agcgccgccc gcgaaggcga
      541 cagcattcgc cgcgccagtc ggcggagcgc ttccaggtgg aggagagcga cggcggctgc
      601 cagggcgatc cgggctgcga acgaccggcc ggcggcgaga tcgaccgcgg cggcgagcca
      661 cggcgcgagc ggaccgtagt agaagcgcac gtcgcgatag agcacgtcgc cgtgcgcgac
      721 gcgccacggc accatgagct cgcggcccgt gtcgacgtac ggctcgatcc agcggttcca
      781 ggaaaccgca gcgagcagga gaaaaacggc gtagggaaga aaggtgaccg cgcggcgggt
      841 cacggtgcgc gcgatcttcc cgcggcgtac gcgagaaagc ccgcggcgac ggcggccgcg
      901 gacgcggcga ggagaaaggt cgccgctcgc ctcgccgaag aggggagctc cggccgggca
      961 ggcacgggcg tttccgccgc ttccgcccgc agcccgcgga tggcccggag gaatgcgacg
     1021 tccgccgccg catcggtccc ttcggcggcg agcgccttcg tcgcttcctc cagcagagcc
     1081 gcggacccgc ccgccttgcg gtaaccctcg agcgccgcgt tgccttccgc gctccgaaac
     1141 gctcccgcca ccgcgcgcga ctcaccctcg gagacccgcg cgagccggtg gaccgcgagc
     1201 tccgcggcga gcctcgccgc cgcggcgggc cggactaccg ccgattcgag agaggccacc
     1261 cggtcgatct tcgtggaaag ggccgctcga aaggaaacga acgaagcgag cgcgagcgcg
     1321 gcggcgatcg ctcccgcggc gatggcagag atcttcacgg cccgatcgta gcaagtgacg
     1381 gcgtccgcgg gcatctcgga acggagcggg gaccttccgg gctacgattc gccgccaatg
     1441 caggagaaga tggcgagccg gaaccccgtc gtgcgtgcga tcgcgtccgc gacgtcgcac
     1501 gtgtggtttc ccctgtccct ctttctcgcg cgccgcctgc cgcgccggct ccttcaggcg
     1561 ctgctcaggc agaccgcgag ccggttcatg cagattcgcc cgaagtacca gagggcgctc
     1621 cgcgcgaacc tgtcgatcat cctcggtcgc gatcccgcct cgcggcaggt gcgccacgcc
     1681 gcgaagggcc tcctcgacgc acacttcacc gcctggctcg acttcctcca cttcgccacc
     1741 cgccccccgg aagaagccgc ccgtctggtc gagagtgtcg tcggctattc gcacatcgtc
     1801 gaggggcgtg cgaagggcaa cggagtcctc ctcctgacgg cgcacctggg caactgggaa
     1861 gtcggcggac tgatgctggc ggagatgcgc gagcccgtgt acgtcgtcct ggtgccggac
     1921 atgttccccg cggtcgagcg cgcgaggcgg gcgttgcacg agcgcgccgg cgttcgcgaa
     1981 atccgggtcg atcgtagctt cgggccgacc ctcgccgttc tgcgggcgtt gaaagaaaac
     2041 gcgatcgtcg cgatgcaggg ggatcgcgac ttcagcaaca ccggagttgc ggtcccgttc
     2101 ttcgggcgcg acgcctactt cccgagaggc cccttcctcg tggcgatggc gactgcggcg
     2161 accgtgctcc ctgcattcat cgtccgcgtt cccgacggtc gttatcgcgc gatcgtcgaa
     2221 gcgccgctcg agatcgaccg cggcagagac cgcgaggcgg cgctcgccgc gaacgtccgc
     2281 cgctacgtgg cgatcctcga gcgctacgtc gccgggcacc ccgagcagtg gtattgcttc
     2341 tatccgttct gggaggatcc gagccgcggg cgccgcgctg acaccgcggc cacgaagaac
     2401 gcgtcgtagt ggcgcgcgag gttctcgacg tcggggagga tctccggctt ttcgaacccc
     2461 gcggcagcga gcgaacggcg ccagctctcc ggcgtcagga agccgtggtt gggccgcgtg
     2521 gccgcgtcgg tcgcgacgcc cgtgaaattc tcgaggaagt tgaagacgaa ctccacgtag
     2581 atcggctggc cctcgaacgg gcgcacgcac tccgagaaga ccgccacgcc gccgggcttc
     2641 aaggtcgcgc ggacctgagc gagcgtcgca gcgaggtctt tcgcgatgtg gatcgtgttc
     2701 accgcgtaca ggacatccgc cgagcccggc tcgatcccct gttcgctgaa gtcgcggttc
     2761 atgtcgagcc gcgagaactc cgccggaagg agcggaaacc gggagcgaac gttgcgctcc
     2821 gcgcgccgca ggaaggtcgg gacgat
//
LOCUS       DASWAV010000256         4257 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U2665
            k141_613777, whole genome shotgun sequence.
ACCESSION   DASWAV010000256 DASWAV010000000
VERSION     DASWAV010000256.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960316
            Sequence Read Archive: SRR10260007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 4257)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4257)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:47:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,929
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4257
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_613777"
                     /isolate="SMAG_U2665"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.36444 N 132.31889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1700)
                     /locus_tag="VF958_05230"
     CDS             complement(<1..1700)
                     /locus_tag="VF958_05230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9148549.1"
                     /translation="MGLPIRRLRNAVFLMVPLLVVASAASATTMGFYTVTPCRVVDTR
                     SPQGPLGGPALTANAERTFTVAGHCGIPAGAGSIAVNVTVSQATAAGNLRLYPSGVSM
                     PASSSVNYAAGQTRANNGVFAVSSSGQLSVYCMQPSGTVQLIIDVVGWFDAAPLPTGT
                     GSQIWARRLSGTGAFDNAYAASLALDGTGNVVVVGAFQGSVDFGGGSLTSAGGTDVFV
                     AKYSASGAHLWSRRFGGASDDYAESVTVDGAGDVAVSGYFSGSADFGSGVLTSAGGSD
                     AFVAKYSSNGAPVWSRRFGGTGTDRALGIGADGSNNLVLTGYFCATVDFGGGPLTSAG
                     LADAFLVKYSSTGGHVWSKRFGGTSSDVPLGIAVADGGNIAMTGYFQGTANFGGQNLT
                     SAGVNDIFAASYDSTGRELWSKAWGNSADDRGTSIAIDGQGSVIVTGSFTGDVDFGGG
                     RILNSGGSDIFLVKYSSNGMHQWSRSYGTTAAVGDIANAVAVDGANNVLLTGSIVGAV
                     DFGGGALAGNGSYDVFVAKFRSDATHVWSKRGGALYDDHGWGIAADSTGNVFSTGDFY
                     "
     gene            1958..2215
                     /locus_tag="VF958_05235"
     CDS             1958..2215
                     /locus_tag="VF958_05235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9148550.1"
                     /translation="MRAWKKVVLALLSAAALLVALFVWKVGPRNVYGLARYGTQRREG
                     DLKLGQPAPDVSLVSLDGKTRVRLSDSTGGKPLVLVFGSYT"
     gene            2228..2614
                     /locus_tag="VF958_05240"
                     /pseudo
     CDS             2228..2614
                     /locus_tag="VF958_05240"
                     /inference="COORDINATES: protein motif:HMM:NF013033.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004800 - thyroxine 5'-deiodinase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="deiodinase-like protein"
     gene            complement(2684..>4257)
                     /locus_tag="VF958_05245"
     CDS             complement(2684..>4257)
                     /locus_tag="VF958_05245"
                     /inference="COORDINATES: protein motif:HMM:NF024758.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HEX9148551.1"
                     /translation="KVGLLRRLESPVRLEGALRELSRGARGDWSGGLLERRTDGALTW
                     TTSLVSPGAVGIRLLIGEATTPGARIYVYSRAGEVHGPYVFDTPIGADGFWTNTVFSD
                     EVFVEVEIPASSTPRLAIDAIAHLEGPALAGAGLGPAGTAGPMSAASVQSPASTTCFV
                     DASCVAPAEFPALESASHAVATLLFRDGLSFYECSGGLVATADRSFEPYLLTANHCFS
                     DQSAAGSLEAVFNYRTSSCNSPYPEMSGFPRTLGSTLLATSSTSDFTLVRLAQPPPSG
                     SFFLGWSSDDVSRSGGTSIYRLSYPAPDVGVWPQYYSRHTISAIPTPGSCSGYAQGNF
                     IYSKDAVGGLSEGSSGAPVLLADGRIVGQLLGACGANVSDDCDSESNSTVDGAFSVSY
                     PQLSQWLNPSGAGGGGPSTCTPSASTLCLNGNRFKVEVAWSVASQGTGGSGVAVPLSA
                     DTGYFWFFSSGNVELVVKALDGRAVNGYYWIFYGALSDVEYTITVTDTSNGAVKTYSS
                     PQGQMASRSDTAAFR"
BASE COUNT          759 a         1401 c         1438 g          659 t
ORIGIN      
        1 tggtagaagt cgcctgtgga gaaaacgttc ccggtcgagt ccgcggcgat gccccagccg
       61 tggtcgtcgt agagcgcgcc gcctcgcttc gaccagacgt gggtggcgtc ggaacggaac
      121 ttcgcgacga agacgtcgta gcttccgttg ccggcgagcg cgcccccgcc gaagtcgacg
      181 gcgcccacga tcgaaccggt gagaaggacg ttgtttgctc cgtcgaccgc cacggcgttc
      241 gcgatgtcgc cgactgccgc cgtcgtgccg tagctccgcg accactggtg cattccgttc
      301 gaggagtact tgacgaggaa gatgtcggaa cccccggaat tcaggatgcg gccgcccccg
      361 aagtcgacgt cgcccgtgaa cgagccggtg acgatcacgc tgccctgacc gtcgatggcg
      421 atcgaggtcc cgcgatcgtc cgcggagttg ccccaggcct tcgaccagag ctcgcggccg
      481 gtcgagtcgt agctcgcggc aaagatgtcg ttcactccgg cgctcgtcag gttctgcccc
      541 ccgaagttcg ccgttccctg gaagtagccg gtcatggcga tgttgccacc gtcggcgacc
      601 gcgattccca gggggacgtc gctcgacgtc ccgccgaaac gcttcgacca gacgtgacca
      661 cccgtcgagg aatacttcac gagaaaggcg tcggcaagcc cggcgctcgt caagggcccg
      721 ccgccgaagt cgacggttgc gcagaagtag ccggtcaaga cgagattgtt cgaaccgtcc
      781 gcgccgatcc cgagagcgcg atcggtcccg gtgcctccga agcggcgcga ccacacgggg
      841 gccccgttcg aggagtactt cgccacgaaa gcgtccgagc ctccggcgct cgtgaggact
      901 ccggagccga agtccgcgga tccgctgaaa tacccggaga cggcgacgtc gcctgctccg
      961 tccacggtga cgctttccgc gtagtcgtcg ctcgcgccgc cgaaccgtcg ggaccagagg
     1021 tgagcgccgg aggcggaata tttcgccacg aacacgtcgg ttccgccggc actcgtcagg
     1081 gagccgccgc cgaaatcgac cgagccctgg aaggcaccga cgacgacgac gttccctgtg
     1141 ccatccagag ccagactggc cgcgtacgcg ttatcgaaag cgccagttcc ggagagccgc
     1201 cgcgcccaga tctgggagcc cgtgccggtc ggcagtggag ccgcgtcgaa ccaaccgacc
     1261 acgtcgatga tgagctggac cgtgccggac ggctgcatgc agtagacaga gagctggccc
     1321 gagctcgaga ccgcgaagac gccgttgttc gcccgcgtct ggccggccgc gtagttcacg
     1381 ctcgagctcg cgggcatcga gacgcccgat ggatacagcc gcaggttgcc ggccgcggtc
     1441 gcctgggaca ccgtgacgtt gacggcgatc gagcccgcgc cggccggaat tccgcagtgc
     1501 ccggcgaccg tgaaggtccg ctcggcgttt gccgtgagtg cggggccgcc gagaggacct
     1561 tgcgggctgc gggtgtccac cacccggcac ggagtcaccg tgtagaagcc catcgtcgtt
     1621 gccgacgccg ccgaagcgac cacgagaagc ggcaccatca agaagacagc gtttcggagg
     1681 cggcggatgg gaagtcccac gcaggttctc ctgaggaagc tttgattacc caacgagttt
     1741 tcctaggacc ttagtcgtag gcaactcgtg ttccatgaac cgatttgttg aaaacaaaga
     1801 gcatggtaac tgccggaccc cccgaatgga cacttatttg tcgcgagaac gctccggacc
     1861 gaccagttct ctgccgtcga tgccctcgcc gatcggggcg aaagaccggc gtagtccccc
     1921 gacgcaggcg ccggagccgg gcgcataatc cccgcgcgtg agagcctgga agaaagtagt
     1981 cctcgctctt ctgagcgcgg ccgccctcct ggtggcgctc ttcgtctgga aggtgggacc
     2041 ccgcaacgtg tacggtctcg cccgctacgg gacccagcgg cgcgagggcg acctgaagct
     2101 cggccagccc gccccggacg tctcgctcgt gtcgctcgac gggaagaccc gcgtccggct
     2161 ctccgattcg acgggcggca agccgctggt gctcgtcttc ggcagctaca cctgacctcc
     2221 gtttcggcgc tcggtcgagc gcgtacgcga gatgcatcgg aagtacgcag atcgcgcccg
     2281 cttcctcacc gtgtacatca aggaggcgca tccggaagac gagtggcaga tggactcgaa
     2341 cgagaaggag gacgtctgtt atccgcagcc gaagacgctc gaacagcggg tcgcgatcgc
     2401 gaacgacttc gtgaagcggt tccactacga cgtgccgatt ctcgtcgacg acatggagaa
     2461 tgccgccaac gaggcgtatg ccggctggcc ggagcgcttc tacgtcctgg acgagagcgg
     2521 aaagatcgtg ttcaagggga agccgggacc gttcggattc caccccgaag aggtcgaagc
     2581 ctggctgaaa gcgaggtttc cggcttcggg gtgagagcct caccaccggc cccctctcag
     2641 cgggagaggg tgccgcctcc ggcgatcaag tgaggcccgg ggtctaccgg aacgccgccg
     2701 tatccgaccg gctcgccatc tgaccctgcg ggctcgagta ggttttcacc gccccgttcg
     2761 acgtgtccgt gacggtgatc gtgtattcga cgtcggaaag cgctccatag aagatccagt
     2821 agtagccgtt caccgcgcgg ccgtcgagcg ctttgacgac gagctcgacg tttccgctcg
     2881 agaagaacca gaagtacccg gtgtccgccg agagaggcac ggcgacaccg cttccgcccg
     2941 ttccctgtga ggccacgctc caggccacct cgaccttgaa ccgattcccg ttcaggcaca
     3001 gcgtagaggc actcggggtg caggtcgatg gcccaccccc gcccgcgccg ctcgggttca
     3061 gccactggga aagctgcggg tagctgacgg agaacgcgcc gtcgacggtc gagttgctct
     3121 cggagtcgca gtcgtcgctc acgttcgccc cgcacgctcc gaggagctga ccgacgattc
     3181 gcccgtccgc gagaaggacg ggtgcgcccg agctcccttc ggagaggccg ccgaccgcgt
     3241 ctttggagta gatgaaattg ccctgcgcgt atccgctgca gctgcccgga gtcggaatcg
     3301 ccgagatcgt gtgccgcgag tagtactgcg gccagacccc gacgtccgga gcggggtagc
     3361 tcagccgata gatcgacgtt cctccactgc gggagacgtc gtccgagctc cagccgagaa
     3421 agaagctgcc ggacggcgga ggctgcgcca gccgaaccag cgtgaaatcg gaggtcgagc
     3481 tcgtggcgag gagcgtcgag ccgagcgtgc gcgggaaacc ggacatttcc ggatacgggc
     3541 tgttgcacga cgaggttcga tagttgaaca cggcttcgag agatcccgcc gcgctctggt
     3601 cgctgaagca gtggttggcc gtcagcaggt acggctcgaa ggaccggtcc gccgttgcga
     3661 cgagaccgcc cgagcactcg tagaaggaga ggccgtcgcg gaagaggagg gtcgcgacgg
     3721 cgtgcgaagc cgattcgaga gccggaaact cggcgggcgc cacgcagctc gcgtccacga
     3781 agcaggtcgt cgatgcgggc gactgtacgg acgctgcgga catcgggccc gcggttccgg
     3841 ccgggccgag ccccgcgccg gcgagggcag gtccctcgag gtgcgcgatc gcgtcgatcg
     3901 cgaggcgtgg cgtcgacgag gccggaatct cgacctccac gaagacctca tcggaaaaaa
     3961 cggtgttcgt ccagaacccg tcggcgccga tcggcgtgtc gaagacgtac ggcccgtgga
     4021 cctcgccggc tcgcgagtac acgtagatgc gtgctcccgg cgtggtcgct tccccgatga
     4081 gcaggcggat gccgaccgcc cccggagaga cgagagaggt cgtccacgtg agtgcgccgt
     4141 ccgtcctccg ttcgagaagg ccaccactcc agtcgccgcg ggcgccgcgc gaaagctctc
     4201 ggagagctcc ttcgaggcgg accggagatt cgaggcggcg caacagaccg accttcg
//
LOCUS       DASWAV010000257         4671 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U2665
            k141_571209, whole genome shotgun sequence.
ACCESSION   DASWAV010000257 DASWAV010000000
VERSION     DASWAV010000257.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960316
            Sequence Read Archive: SRR10260007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 4671)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4671)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:47:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,929
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4671
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_571209"
                     /isolate="SMAG_U2665"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.36444 N 132.31889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2310)
                     /locus_tag="VF958_05250"
     CDS             complement(<1..2310)
                     /locus_tag="VF958_05250"
                     /inference="COORDINATES: protein motif:HMM:NF014516.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006302 - double-strand break repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEX9148552.1"
                     /translation="MLRLERVEISGFKSFYEKVDLAFPGRVTAVVGPNGCGKSNICDA
                     VAWALGEQSARILRGERMDDVIFNGSAKRRPLGMAEVIVTLAPEKGNGNGNGNGDGEP
                     ERLRVGRRVFRDGEGEYFLNDKQVRFKDVQDRLLGTGLSVRTYSVIEQGRIDQVLSTK
                     PQDRRRLIEEAAGITRYKAKRRLAEIKLEETRANLLRISDIVSEVERNCASLKRQASR
                     AARHKEMSESLRVRRAALARVRSDAMVEALAVAERLRDDRRDAEAAAAAALGHAEAAL
                     AAARIAAGETAAERDRLRELLAALEAAIARDDERLEANHRSSSDLAARRSASEREGRD
                     FSGEETRTARELSELAARLERAVGEARIRSLAREESDRRAAEAAAALAAIETELEKAR
                     EQALRATGERVAARNARHDLELAAERIAAGVARLGESLAKLAGELADRRSDLDGAERE
                     QHALADRAAASEEAVRRLSEEIGGLSRSIAETETSRENARASLAVLEERASSLDEALR
                     AREGTLAKLAEALSGAGLESEGTLSRRLQPAPGWEHAVDVVLGGDLDALLARGDWRRA
                     ATAARDLPGPRLLRADWRLSPAAAPPIAGTLGGWEIALSNHGRLTPAERSALPPVAFV
                     ASLDSALSLAEAFPEALFVTRDGDVARGPVLRFAGTRVRTSSAFALERELADSAGRTE
                     AARTAIASMEASRANLESARGKKEQELTEARETEGRRQSELSAFSARVEERRAAVARL
                     SLETATSSTEREGLTAEAAALEARRRVVED"
     gene            2736..3797
                     /locus_tag="VF958_05255"
     CDS             2736..3797
                     /locus_tag="VF958_05255"
                     /inference="COORDINATES: protein motif:HMM:NF014950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX9148553.1"
                     /translation="MLNLEVRKKIGGGEVHRLDGSAITIGASSGNEVVVRARGVAGRH
                     VRISQREGGYHLDLYKGASLISLNGREFGGGPIGIGDRITIGEATITVLHAQPNLLRA
                     LTESAIVAEPAPPGPPLPATEVEFRDLRLTAYRVCRESGSREELATELADFLDGAFPP
                     TEWAVGEFSAAGFRPLASTFRESPALPPSVVEEARSGERLVRMDTVSGTLTLVVEPPL
                     NGSVLLAVLVRETPRLPARAVLFLEEVIQLAGIAFSSRARLACPAAEKTPHAEGSEAA
                     SREPSGAEAMLRQTDDLKVIVETVEREVIDRAMKRVEGNQSRGAHVLNISRGSLIAKL
                     KEYSIPDYRYLRRERTRRY"
     gene            complement(3826..4404)
                     /locus_tag="VF958_05260"
     CDS             complement(3826..4404)
                     /locus_tag="VF958_05260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9148554.1"
                     /translation="MLTPRALLRALVGFLLAIAFWFGFSRPYEATVAVAAQTLTNVFE
                     SPDVTRLKAGKGEFLLDRRDFPPGSARPGLPAPDIHFNFVLLAALFAMDRRPLEAAHV
                     TRFLAAAGILWVVHVLALAFQVQSIYATSLGAWSDAHYGRLSSNFWAAGWHFYQIAGR
                     FAAPFALWWFFGRREEDDSEKKGRSGRKRKRN"
     gene            complement(4444..>4671)
                     /locus_tag="VF958_05265"
     CDS             complement(4444..>4671)
                     /locus_tag="VF958_05265"
                     /inference="COORDINATES: protein motif:HMM:TIGR04178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exosortase H"
                     /protein_id="HEX9148555.1"
                     /translation="GLVAGFVAIQVLNLFRVISLFWIGLHRPALFSSSHTVIWQSVVV
                     LFGVLLFLVWASRERRLSAESSSAGERPRRG"
BASE COUNT          736 a         1619 c         1592 g          724 t
ORIGIN      
        1 gtcctcgacg acccggcggc gcgcttcgag cgccgcggct tccgctgtga ggccttcccg
       61 ttcggtcgag gacgtcgcgg tttccaggga cagccgggcc accgcggcgc ggcgctcttc
      121 gacgcgtgcg gaaaaggcgg agagctcgga ctgcctgcgg ccctcggtct cccgcgcctc
      181 cgtgagctcc tgctctttct tcccgcgagc ggactccagg tttgcccgcg aagcctccat
      241 cgaagcgatt gccgtccgcg cggcttcggt ccttcccgcg ctgtcggcca gctcgcgttc
      301 gagcgcgaag gcgctggacg tgcgaacccg tgttccagcg aatcggagca cgggtccgcg
      361 ggcgacgtcg ccgtcacggg tcacgaagag cgcctcgggg aaggcctccg cgagagaaag
      421 cgcggagtcg agcgacgcga cgaacgcgac gggtggaagc gcggatcgct ccgcgggagt
      481 gagccgtccg tggttcgaga gcgcgatctc ccagccgccg agcgttcccg cgatcggcgg
      541 agcggccgcc ggagagagcc gccagtcggc gcgcagcagc cgcgggccgg ggaggtcgcg
      601 agccgccgtc gccgctctcc tccagtctcc gcgcgcgagg agcgcgtcga ggtcgccgcc
      661 gaggacgacg tcgacggcgt gctcccatcc gggcgccggc tgcagccggc gggagagagt
      721 cccctcgctc tcgagccccg cgccggagag cgcctcggcg agctttgcga gcgtgccctc
      781 gcgcgcgcgc agcgcttcgt cgagagacga cgcgcgctct tcgagtaccg cgagagaagc
      841 acgggcgttc tctcgcgagg tctcggtctc cgctatggaa cgcgagagcc cgccgatctc
      901 ctcggagaga cgacgaaccg cctcttccga cgccgccgcg cgatcggcga gggcgtgctg
      961 ctcgcgttcg gcaccgtcca aatccgagcg ccggtcggcg agctcgccgg cgagcttcgc
     1021 gaggctctcg ccgagccgtg cgacgcccgc cgcgatgcgc tcggccgcga gctcgagatc
     1081 gtgccgggcg ttgcgggccg cgactcgctc gccggttgcc cgcagcgcct gctcccgggc
     1141 cttctccagc tccgtctcga tggcggcgag cgcggcggcg gcctccgccg cgcgccggtc
     1201 ggactcctcg cgggcaagcg accggatgcg agcttctccg accgctctct cgagtctcgc
     1261 ggcgagctcc gagagctccc gcgccgttcg cgtctcttct cccgagaaat cgcgcccctc
     1321 gcgttccgat gcggagcgtc gcgccgcgag atccgatgac gagcggtggt tcgcttcgag
     1381 ccgttcgtcg tcccgcgcga tcgccgcttc gagcgccgcg aggagctcgc gcagccggtc
     1441 ccgctccgcg gcggtctccc cggccgcgat ccgcgccgcc gcgagcgccg cctccgcgtg
     1501 tccgagagcc gccgcggcgg ccgcctcggc gtcgcgcctg tcgtcgcgca acctttcggc
     1561 gacggcgagg gcttcgacca tcgcgtccga ccggacacgc gcaagcgccg cgcggcgaac
     1621 gcgcagcgac tcgctcatct ccttgtgccg cgccgctcgc gacgcctggc gcttcaacga
     1681 ggcgcagttg cgttcgacct cgctcacgat gtcggagatg cggaggagat tcgctcgggt
     1741 ctcctcgagc ttgatctcgg cgagccggcg cttcgctttg tacctcgtaa tgccggcggc
     1801 ctcttcgatc aggcggcggc ggtcctgcgg cttcgtggac agcacctggt cgatccggcc
     1861 ctgctcgatg acggagtacg tccgcaccga gaggccggtg ccgaggagac ggtcctgcac
     1921 atccttgaaa cggacctgct tgtcgttcaa gaagtactcg ccctcgccgt cgcggaaaac
     1981 ccggcgaccg acgcggaggc gttccggctc gccgtcccca ttgccgttgc cgtttccgtt
     2041 tcccttttcg ggcgcgagcg tgacgatcac ctccgccatt ccgagcgggc gtcgtttggc
     2101 ggagccgttg aagatcacgt cgtccatgcg ctcgccgcgc aggatgcggg cgctctgctc
     2161 tcccagagcc catgcgaccg cgtcgcagat gttggatttg ccgcaaccgt tcgggccgac
     2221 gaccgcggtc acccggcccg ggaacgcgag atcgaccttc tcgtagaagg acttgaagcc
     2281 ggagatctcc acccgttcca ggcgcagcat gggagttgcg gaagaattta acagatttgg
     2341 cggctaagtt ccacaaaatg tgggggtaac gaccgcagct cccccaagat ctcgatgacg
     2401 catccttgcc ccgtccgggc acgggccacg cttctccggc gacgctcgct gactccgccg
     2461 cgccggattt cgtcggccgg ctgccgtgaa acatcagcgc gaaaacgcga gtcgagggag
     2521 gcggcgccga tccttcgctc cttctgatcc gccgctccat tgcgcaggag ttcggcgtga
     2581 cataatccaa acaggcttat cccaaatccg gacgattcga atcgactgac ggggagagtt
     2641 cctcttgcgc gagaacaatc tcgaaaaagc gaggcacttt tgcgcgggac gcgcggacaa
     2701 ctgtcggaac gtttgacgca aaaggacgaa tcgacatgct gaacctggag gtccgcaaga
     2761 agatcggggg aggcgaagtt caccgcctgg acggttcggc gatcacgatc ggcgcttcgt
     2821 cgggaaacga ggtcgtcgtg cgagcgcgcg gggtcgcggg ccgccacgtc cgaatcagcc
     2881 aacgcgaagg cggttaccac ctcgacctct acaagggcgc gagtctcatc agcctgaacg
     2941 gtcgcgagtt cgggggcggt ccaatcggca tcggagatcg gatcaccatc ggagaggcaa
     3001 cgatcaccgt gctccacgcg cagccgaacc ttctgcgcgc gctgaccgaa tcggctatcg
     3061 ttgcggagcc ggcgccgccc ggcccgccgc tccccgcgac ggaggtggaa ttccgggacc
     3121 tccgtctgac tgcttaccga gtctgccgtg aaagcggatc gcgggaagag ctcgcgaccg
     3181 aactcgccga ctttctcgac ggcgcgtttc cccccaccga atgggcggtg ggggagttct
     3241 cggcggcggg atttcgcccg ctggcgtcca cgttccgcga gtcgccggcg ctcccgccga
     3301 gcgtggtcga ggaggcgcgg agcggggagc gtctcgtacg gatggacacg gtttccggca
     3361 ctctcaccct cgtcgtcgag ccaccgctca acgggtccgt cttgctcgcc gtcctggtcc
     3421 gcgagacacc ccgccttccc gcgcgagccg tgctgttcct cgaagaggtg atccagctcg
     3481 ccgggatcgc cttctcatcg cgagcgcgcc tcgcttgccc ggcggcggag aagactccgc
     3541 atgcggaagg ttccgaggct gcgtcgaggg aaccctccgg cgcggaggcg atgctccgtc
     3601 agaccgacga tctgaaggtg atcgtcgaga ccgtcgaacg cgaagtcatc gaccgcgcga
     3661 tgaaacgcgt cgagggcaat caaagccgcg gcgctcacgt tctcaacatc tcgcgcggct
     3721 cgctcatcgc gaagttgaag gagtattcga tacccgacta tcggtacctg cggcgcgaga
     3781 ggactcgacg gtactgagcc ggcagccgca cgcaactgtc gtccgctagt ttctttttct
     3841 ctttcttccg ctccggccct tcttctcgga gtcgtcctct tcgcggcgac cgaagaacca
     3901 ccacagcgca aacggcgcgg cgaagcggcc ggcaatctgg tagaagtgcc agcccgccgc
     3961 ccagaagttg ctcgagagcc ggccgtagtg cgcgtcgctc caggccccga ggctcgtcgc
     4021 atagatcgat tggacctgga aggcgagcgc gagaacgtga acgacccaga ggattcccgc
     4081 ggccgcgagg aaccgcgtca cgtgtgcggc ctcgagcggg cgccgatcca tcgcgaagag
     4141 cgcagcgagc aggacaaagt tgaagtgaat gtccggggcc ggcaagccgg ggcgcgcgga
     4201 acctggcggg aagtcgcgcc gatccaggag aaactccccc tttccggcct tcagccgcgt
     4261 cacgtccggc gactcgaaaa cgttcgtcag ggtctgcgcg gcgacggcaa ccgtcgcctc
     4321 gtaggggcgc gagaagccga accagaaggc gatcgcgagg aggaacccca cgagcgcacg
     4381 cagcagggcg cgaggcgtga gcactggaga ccgcacgccc ggtgactgcg ctcgcgcggc
     4441 cgttcaaccc ctccgggggc gttctcctgc cgacgaggat tcggcggaga gtcgcctttc
     4501 gcgcgacgcc cagaccagga agaggagcac gccgaagagg accaccacgg actgccagat
     4561 gacggtgtgg gacgagctga agagcgcggg gcggtggagg ccaatccaga agagcgagat
     4621 gacccggaac aggttcagca cctggatcgc gacgaacccg gcgacgagtc c
//
LOCUS       DASWAV010000258         3764 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U2665
            k141_494233, whole genome shotgun sequence.
ACCESSION   DASWAV010000258 DASWAV010000000
VERSION     DASWAV010000258.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960316
            Sequence Read Archive: SRR10260007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 3764)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3764)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:47:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,929
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3764
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_494233"
                     /isolate="SMAG_U2665"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.36444 N 132.31889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..582
                     /locus_tag="VF958_05270"
     CDS             <1..582
                     /locus_tag="VF958_05270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase family 2"
                     /protein_id="HEX9148556.1"
                     /translation="EAFFHLTGNFSYLLVVLLALLLLPAIVIREEIGWRKLAVLDFPL
                     FFGATFSFVAFYVSSQREIGQTWGRTLKYMPCLMSLGIGLSLTNMQAVLEGLGNKPAE
                     FTRTPKYSIEGAGGEWRSKKYRSASNFTLAAEILLSGYFLAALAFGVAERYWVGVPFL
                     LLFFNGFVYTAASSLQSRWDDGAREQELALAEP"
     gene            534..1247
                     /locus_tag="VF958_05275"
     CDS             534..1247
                     /locus_tag="VF958_05275"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HEX9148557.1"
                     /translation="MGRRSTRARARPRGALSPSPESRLEPTLRVTEIFRSIQGESTRA
                     GFPCAFIRLTGCSLRCVWCDTAYAFSGGRDMTVSEAVDRVLALGTDLVEITGGEPLEQ
                     EAVYTLLDRLLDRGKTVLVETGGHVPLDRIDPRAVKIVDVKAPGSGMVAANLRENLER
                     LGPRDELKFVLADRRDFDWALDFVRERGLDRRHAVTFSPVWETLPAGELAEWIRDSGR
                     PIRLGVQLHKLLWGDVPGR"
     gene            1279..1965
                     /gene="queC"
                     /locus_tag="VF958_05280"
     CDS             1279..1965
                     /gene="queC"
                     /locus_tag="VF958_05280"
                     /EC_number="6.3.4.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012469277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine synthase QueC"
                     /protein_id="HEX9148558.1"
                     /translation="MNRAIVLLSGGLDSATCLLIARKEADAVFALSFDYGQRHKVELE
                     RARALVGRYGAAAHRVVRLDYPAPENSALTDASREVPRHALGVEPIPVTYVPARNTLF
                     LAHAVAWGESIGAADIYIGANALDYSGYPDCRPEFLESFERTANLGTKAGVEGSLSFR
                     IQAPLLSMSKAEIVALAGRLGLDFTLTSSCYDPSPSGAPCGACDSCLLRAKGFQQAGL
                     ADPLSPAGPA"
     gene            2095..>3764
                     /locus_tag="VF958_05285"
     CDS             2095..>3764
                     /locus_tag="VF958_05285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013986114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Clp protease N-terminal domain-containing
                     protein"
                     /protein_id="HEX9148559.1"
                     /translation="MRFDQLTIKAQEAVQEAQRDARARGNAELSTDHLLLALLRQDEG
                     VVAPILQKLGVDPEALAREVEADLDKRPKVTGASADAMVSLDLNRVFDRAFELMPEFG
                     DEYVSTEHFLLALAEPAAGATSKRLASKGAAKDALLKALKEVRGSSRVTDPTPEDKYQ
                     ALKRYTRDLTDLARRGKIDPVIGRDEEIRRVIQVLSRRTKNNPVLIGEPGVGKTAIVE
                     GLARRIHMGDVPEGLKNKRVVALDLGAMIAGAKYRGEFEDRLKAVLKEIEEAAGTVVL
                     FIDELHTLVGAGAAEGAMDASNMLKPALARGELRAIGATTLSEYQKYIEKDAALERRF
                     QPVFVDEPSVEDTIAILRGLQERYEVHHGVRITDGALVAAARLSHRYISDRFLPDKAI
                     DLVDEAAAKLRTEIDSVPTEIDEIERRIMSLDIEKKALEKEKTKEAKERLEEIDRVLA
                     EEREKASRLRAQWSAEKEEIGKIRQIKESIETVRLQATEAEREGNLQRAAELRYGRLP
                     ELERELAANNTRLADLQKGGRPMLREEVTEEDIAAVVSRWTGVPVSRML"
BASE COUNT          642 a         1295 c         1234 g          593 t
ORIGIN      
        1 gaggcgttct tccacctgac cggcaacttc tcctacctgc tcgtcgtcct gctcgctctc
       61 ctcctcctgc ccgccatcgt cattcgggag gagatcggct ggcgcaagct cgcggtcctc
      121 gactttcccc tgttcttcgg cgccaccttt tccttcgtcg ccttctacgt ctcctcgcaa
      181 agagagatcg gacagacctg ggggaggacg ctcaagtaca tgccgtgcct gatgtcgctc
      241 ggaatcggac tgtctctgac gaacatgcag gccgtgctcg aagggctcgg gaacaagccg
      301 gccgagttca ctcggacgcc gaagtactcg attgaagggg ccgggggcga gtggcggagc
      361 aagaagtacc ggtcggcctc gaacttcact ctggccgccg aaatcctgct cagcggctac
      421 ttcctcgccg cgctcgcgtt cggggtggcg gagcgatact gggtcggcgt ccccttcctc
      481 ctccttttct tcaacggctt cgtgtacacg gcggcgtcgt cgctccagtc ccgatgggac
      541 gacggagcac gagagcaaga gctcgccctc gcggagccct gagtccgagc ccggagtccc
      601 gactggaacc gactcttcgc gtcaccgaga tcttccgatc catccagggg gagtcgaccc
      661 gcgccggctt tccctgcgcg ttcatccgcc tgaccggttg ttccctccgc tgcgtctggt
      721 gcgacaccgc gtacgcattt tcgggggggc gggacatgac ggtctcggaa gctgtcgacc
      781 gcgtcctcgc gctcggaacc gacctcgtcg agatcaccgg cggcgagccg ctcgagcagg
      841 aagccgtcta cacgctcctg gaccgcctcc tcgaccgcgg gaagacggtc ctcgtggaga
      901 ccgggggtca cgtcccgctc gaccggatcg acccgcgcgc cgtgaaaatc gtggacgtca
      961 aggcgccggg cagcggaatg gtggcggcga accttcgcga aaacctggag cgtctcgggc
     1021 cgcgcgacga gctcaagttc gtcctcgccg accggcgcga cttcgactgg gcgctcgact
     1081 tcgtccgcga acgcggcctc gaccggcgac acgccgtcac gttctctccg gtgtgggaga
     1141 cgctccccgc gggagagctg gccgagtgga tccgcgactc cggacggccc atccggctcg
     1201 gcgtccagct ccacaagctc ctctgggggg acgtgccggg ccggtgaccg aaaaagacca
     1261 gaatgcctgc ctgaccccat gaaccgggcg atcgttcttc tctccggcgg gctcgactcg
     1321 gcgacctgcc tgctcatcgc acggaaggaa gccgacgcgg tcttcgcgct ctccttcgac
     1381 tacgggcagc ggcacaaggt cgagctcgag cgagcgcgcg cgctcgtcgg ccggtacggc
     1441 gcggccgcgc accgggtcgt gcggctcgac taccccgccc ctgagaactc ggcgctgacg
     1501 gacgcgtccc gcgaggtccc ccggcacgca ctcggcgtcg agccgattcc ggtcacgtac
     1561 gtccctgctc gcaacactct cttcctcgcg cacgcggtgg cgtggggaga gagcatcggg
     1621 gcggcagaca tctatatcgg tgcgaacgcg ctcgactact ccgggtatcc ggactgccgg
     1681 ccggagtttc tcgagtcctt cgagcggacc gcgaacctcg ggacgaaggc gggggtcgaa
     1741 gggagcctct cctttcgcat ccaggcgcct ctcctctcga tgagcaaggc cgagatcgtc
     1801 gcgctcgccg ggcggctggg tctcgacttc acgctcacgt ccagctgcta cgacccctcc
     1861 ccgtccgggg ctccgtgcgg cgcctgcgac tcctgcctgc tccgagccaa aggcttccag
     1921 caggcaggtt tagccgatcc gctgtccccg gccggcccgg catagcccta atccataatt
     1981 cttgcgtcga gcaggctcaa ctatgcatcc atgaaaactt gacatgttgg gacgcatgtt
     2041 gtaaattgcg gcccgtcgga agcgaaggaa gctcattccg gaggtcccac ttcgatgcgg
     2101 ttcgatcaac tcacgatcaa agctcaggag gccgtccagg aagcccagcg cgacgcccgc
     2161 gcccgcggca atgcggagct ttcgaccgac cacctgctgc tcgcgctcct ccgccaggac
     2221 gagggcgtcg tcgctccgat tctccagaag ctcggcgtcg accccgaggc ccttgcgcgc
     2281 gaggtcgagg cggacctcga caaacggccg aaggtcacgg gcgcgtccgc cgacgccatg
     2341 gtgtcgctcg acctgaaccg cgtcttcgac cgggcgttcg agctcatgcc cgagttcggc
     2401 gacgagtacg tctcgaccga acactttctg ctcgcgctcg cggagcccgc cgccggggcg
     2461 acatcgaagc ggctcgcgtc gaagggcgcg gcgaaggacg ccctcctgaa ggctctcaag
     2521 gaagtccgcg gctccagccg cgtcacggat ccgacgccgg aggacaagta ccaggcgctc
     2581 aaacgctaca cgcgggacct caccgacctc gcgcggcgcg ggaagatcga tcccgtcatc
     2641 ggccgcgacg aggagatccg tcgcgtgatc caggttctct cgcgccgcac gaagaacaac
     2701 ccggtcctga tcggcgagcc cggcgtcgga aagaccgcca tcgtcgaggg gctcgcccgc
     2761 cggatccaca tgggcgacgt gcccgaaggg ctcaagaaca agcgcgtcgt cgccctcgac
     2821 ctcggcgcga tgatcgccgg cgccaagtat cgcggcgagt tcgaggaccg gctgaaagcg
     2881 gtgctcaagg agatcgaaga ggctgcgggg accgtcgtcc tcttcatcga cgagctgcac
     2941 acgctcgtcg gcgcgggcgc cgcggaaggc gcgatggacg cctccaacat gctgaagccg
     3001 gcgctcgccc gcggcgagct gcgcgcgatc ggcgcgacga cgctctccga gtaccagaaa
     3061 tacatcgaga aggacgcggc gctggagcga cgcttccagc ccgtcttcgt cgacgaacct
     3121 tcggtcgaag acacgattgc gatccttcgc ggcctccagg agcgctacga ggtccaccac
     3181 ggggtccgga tcaccgacgg cgctctcgtc gccgcggcgc gcctctccca ccgctacatc
     3241 agtgaccgtt tcctcccgga caaggcgatc gacctcgtgg acgaggcggc ggcgaaactg
     3301 cgcaccgaga tcgactcggt gcccaccgag atcgacgaga tcgaacggcg gatcatgtcc
     3361 ctcgacatcg agaagaaggc gctcgagaag gaaaagacga aagaggcgaa ggagcggctc
     3421 gaggagatcg atcgcgttct cgccgaggag cgggagaagg cgagccgcct gcgcgcccag
     3481 tggtctgcgg agaaggaaga gatcggaaag atccgtcaga tcaaggagtc gatcgagacg
     3541 gtccgactgc aggcgaccga ggcggagcgg gaggggaacc tccagagggc ggcggaactt
     3601 cggtacggac gcctgcccga gctcgagaga gagctcgccg cgaacaatac gcgtctcgcg
     3661 gacctgcaga agggcggccg tccgatgctc cgcgaggagg tcaccgagga ggacatcgcg
     3721 gcggtcgtgt cgcgctggac cggcgtgccc gtctcccgga tgct
//
LOCUS       DASWAV010000259         1978 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U2665
            k141_613956, whole genome shotgun sequence.
ACCESSION   DASWAV010000259 DASWAV010000000
VERSION     DASWAV010000259.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960316
            Sequence Read Archive: SRR10260007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 1978)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1978)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:47:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,929
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1978
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_613956"
                     /isolate="SMAG_U2665"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.36444 N 132.31889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..450)
                     /locus_tag="VF958_05290"
     CDS             complement(<1..450)
                     /locus_tag="VF958_05290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014807032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 6-dehydrogenase"
                     /protein_id="HEX9148560.1"
                     /translation="MNICVVGTGYVGLVTGACLADFGMNVVCVDKDDSKIGELQSGRI
                     PIYEPGLEEIVAKNERAGRLRFTTDLKAGIEGALAIFIAVGTPPNPDGSPDLTFVRQV
                     AEAIAQYMNGYKVVVTKSTVPTGTGRMIEQILKSNDGEREFSVVSNPE"
     gene            complement(447..1460)
                     /locus_tag="VF958_05295"
     CDS             complement(447..1460)
                     /locus_tag="VF958_05295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007416960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HEX9148561.1"
                     /translation="MNVLVTGGAGFIGSHVAGRRLARGDRVVVLDDFNDFYDPALKRA
                     NVEPLRKSPRFRLVEGDIRDRGLVESAFADERFDAVVHLAARAGVRPSLSQPVLYEEV
                     NCVGTLHLLEAAVAHGRPLFLFASSSSVYGVNSKLPFSEDDPVERPISPYATTKRAGE
                     LHVFTAHYLHRLAAWCLRFFTVYGPRQRPEMAISRFIRSIESGEAIPFFGDGSSRRDY
                     TYIDDIADGVEAALDRGAPASGFEIVNLGGAHPVTLSDLVVAIERATGRKAILDRQPL
                     QPGDVPVTYASVEKAERLLGFRARVTLADGLRRTLDWFRNTAAREPHEIRSPGGEKNG
                     TVK"
     gene            complement(1457..>1978)
                     /locus_tag="VF958_05300"
     CDS             complement(1457..>1978)
                     /locus_tag="VF958_05300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9148562.1"
                     /translation="LRNVFGKIEKDMASGNGMIPALLSDPEGKKRVYDLLDKLGATAG
                     SLAAVSENLNTGKGALPTLLHDEAFSREFTGNLRSLSKRLDSIARKLDEGQGTVGKLI
                     NDPAIFDAANHLVVGIDESKLLRWLIRNRQRSGIRKRYQEAQTQSAQNPPPEGVGSVE
                     ATPLPAPTATPKP"
BASE COUNT          329 a          644 c          654 g          351 t
ORIGIN      
        1 ctccgggttc gacacgacgg agaactcccg ctccccatcg ttcgacttca atatctgctc
       61 gatcatccgc cccgtgccgg tcggtacggt ggacttcgtc accacgacct tgtagccgtt
      121 catgtactgc gcgatcgcct ctgcgacctg ccggacgaac gtcaggtccg gagagccgtc
      181 ggggttcggc ggagtcccga ccgcgatgaa gatcgcgagc gctccctcga tgccggcctt
      241 caggtccgtc gtgaaccgaa gtctcccggc ccgctcgttc ttggcgacga tctcctcgag
      301 acccggttcg tagatcggta tccgacccga ctgaagctcc ccgatcttcg aatcgtcctt
      361 gtcgacgcag accacgttca tcccgaagtc cgcgagacac gctccggtca cgagtccgac
      421 gtagcccgtg cccacgacgc agatgttcat ttcaccgttc cgttcttttc gcctccgggc
      481 gagcggatct cgtgcggctc gcgcgccgcg gtgttgcgaa accagtccag cgtccggcgc
      541 agcccgtccg cgagagtaac gcgagcccgg aaacccagga ggcgctcggc cttttcgacg
      601 gaagcatacg tgaccggaac gtcgcccggc tgcagcggct gtcggtcgag aatcgccttg
      661 cgaccggtcg cccgctcgat cgcgacgacg aggtccgaca aggtgaccgg gtgcgctcct
      721 cccaggttga cgatctcgaa gccgctcgcc ggcgctcccc gatcgagcgc cgcttcgacg
      781 ccatcggcga tgtcgtcgat gtacgtgtag tcgcggcggg agctgccgtc accgaagaac
      841 gggatcgcct cgccgctctc gatcgatcgg atgaagcgcg agatggccat ttcgggccgc
      901 tggcgggggc cataaacggt gaagaaccgg aggcaccatg cggccaggcg gtgaaggtag
      961 tgcgcggtaa agacgtgcag ttctcctgcc cgcttcgtcg tcgcgtacgg cgaaatcggc
     1021 cgctcgacgg gatcgtcctc ggagaagggg agcttcgagt tgactccgta gaccgaggag
     1081 gacgacgcga agagaaagag aggtcgtccg tgagcgactg cggcctcgag caggtgcaga
     1141 gtgccgacgc agttgacctc ttcgtagagg accggctggg aaagcgaagg ccggacgccg
     1201 gcccgggcgg cgaggtgcac gacggcgtcg aaccgttcgt cggcaaacgc gctctcgacg
     1261 aggccgcggt cgcgaatgtc gccctcgacg agacggaaac gcgggctctt ccggagaggc
     1321 tcgacgttcg cgcgcttcag cgccgggtcg tagaagtcgt tgaagtcgtc gagcaccacg
     1381 acccggtcgc cacgcgcgag gcggcgaccg gcgacgtggg agccgatgaa tccggccccc
     1441 cccgtaacga ggacgttcaa ggtttcggcg tcgccgtggg cgccggcaga ggagtggctt
     1501 cgaccgagcc gactccttcc ggcggcggat tctgcgccga ttgggtctgg gcctcctggt
     1561 agcgcttccg gattccggat cgctggcggt tgcggatcag ccagcgcaga agcttcgact
     1621 catcgattcc gacgacgaga tggtttgccg cgtcgaaaat cgccgggtcg ttgatcagct
     1681 tcccgacggt tccctgtccc tcgtcgagct tgcgggcaat cgagtccagt cgcttcgaca
     1741 ggctccggag gttgcccgtg aactcgcggg agaaagcctc gtcgtggagg agcgtcggaa
     1801 gcgcaccttt gccggtgttc aggttctcgg agaccgccgc aagagatccc gcggtcgcgc
     1861 cgagcttgtc gaggaggtca taaaccctct tcttgccctc gggatccgag agcagcgccg
     1921 ggatcatccc gtttccgctc gccatgtcct tctcgatctt gccgaaaacg ttgcgcag
//
LOCUS       DASWAV010000260         2370 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U2665
            k141_332841, whole genome shotgun sequence.
ACCESSION   DASWAV010000260 DASWAV010000000
VERSION     DASWAV010000260.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960316
            Sequence Read Archive: SRR10260007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2370)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2370)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:47:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,929
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2370
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_332841"
                     /isolate="SMAG_U2665"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.36444 N 132.31889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..690
                     /locus_tag="VF958_05305"
     CDS             <1..690
                     /locus_tag="VF958_05305"
                     /inference="COORDINATES: protein motif:HMM:NF013250.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HEX9148563.1"
                     /translation="SATWAGAILGAIAYFFGNVLDCADGQLARLQSSASPIGYLVDGA
                     ADYLGTTAVFVGMAHWLNLQRPAPLNWWWLVVAAGISMAWQCAFLEEKRKEWQRRVYQ
                     RSRDRKAELEALARQAAQWRAERTHLAGRVLIAGYRAYRTGWAPLTRSAVETSAPDAG
                     DETRWVECHLPVLRRSVWIGPTMHVTAIVLSTGLLNRPDWYLWAATTIGNLWMIATLV
                     AERRAARCMAE"
     gene            687..1418
                     /locus_tag="VF958_05310"
     CDS             687..1418
                     /locus_tag="VF958_05310"
                     /inference="COORDINATES: protein motif:HMM:NF024214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphocholine cytidylyltransferase family
                     protein"
                     /protein_id="HEX9148564.1"
                     /translation="MKVRVRAIVLVAGAARRLHPLTLERPKCLLPVAGRTILSRAVSI
                     LADRGITRFTVVDGFACDQVRAALTSEFPPGWFTFVHNPDFANTNNAYSLWLARLPDE
                     DPIVLLDGDLVFDPEVLDLLLEDRHPNRLAVRTRGDVGEEDVKVVLGPDGRISDIGKH
                     VSLGRTGGESVGIAAFSAPFARRLFDVLERRVSREGGRDEWYESAFVELIEAGELVYP
                     VDLGELQAIEVDTADDLQRARNLFV"
     gene            complement(1428..2045)
                     /locus_tag="VF958_05315"
     CDS             complement(1428..2045)
                     /locus_tag="VF958_05315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006944149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HEX9148565.1"
                     /translation="MRRQQDVGRDEAAKAVGVSRSLAGFHLDRLVEEGLLEASFRRLS
                     GRAGPGAGRPAKVYRRSRRQLELSVPQRRYELAARILATAVDTSAARDTRGALKKSAR
                     RIGAQIGGEMRSQSGLRPARKRLIAGMVDALASQGYEPEATPGAVRFRNCPFDALVGE
                     HRELVCGMNLALIEGVVQGIDLPGVRPVLAPEPGMCCVRLELPKR"
BASE COUNT          371 a          821 c          759 g          419 t
ORIGIN      
        1 agcgcgacat gggccggcgc cattctcggg gcgatcgcct actttttcgg gaacgtgctc
       61 gattgcgccg acggtcagct cgcgcgactc cagagctccg catctccgat cggatatctc
      121 gtggacggag ccgcggacta cctaggtacg accgcagtct ttgtgggaat ggcccactgg
      181 ctcaaccttc aacgcccggc gcccctgaac tggtggtggc tcgtcgtcgc cgcgggaatt
      241 tcgatggcct ggcagtgcgc ttttcttgaa gagaaacgga aggagtggca aaggcgtgtc
      301 tatcaacgct cgcgagaccg caaggcggag ctcgaagcgc tcgcgcgcca ggctgctcaa
      361 tggcgcgcgg aaagaactca cctcgccgga cgggtcctga tcgcaggcta ccgcgcttac
      421 cgaaccggct gggctccgct cacccgatcg gcggtggaaa cgtcggcgcc cgacgccgga
      481 gacgagactc ggtgggtgga gtgccacctg ccggtcctgc gccggagcgt ctggatcggg
      541 ccgacgatgc acgtcacggc gatcgtgcta agcacgggcc tgctgaatcg ccccgattgg
      601 tatctctggg cggcgacgac gatcggcaac ctgtggatga tcgcgacgct cgtcgctgag
      661 cggcgcgccg cgcgctgcat ggccgagtga aggtacgcgt ccgcgcgatc gtgctcgtgg
      721 ccggcgccgc ccggcggctt catcccctga ccctcgagcg acccaagtgc cttcttccgg
      781 tcgccggccg cacgatcctc tcccgcgccg tctcgatcct ggccgatcgg ggaatcacgc
      841 ggttcacggt cgtggacggg ttcgcctgcg atcaggtccg ggccgcgctc acgtccgagt
      901 ttcccccggg ctggttcacg ttcgttcaca atccggactt cgcgaacacg aacaacgcct
      961 actctctctg gctggcccgg ttgcccgatg aggatccgat cgttctcctc gacggggacc
     1021 tggttttcga tccggaagtc ctcgatctgc tcctggagga tcggcacccc aaccgcctcg
     1081 cagtgcgaac ccgcggtgac gtcggggaag aggacgtcaa ggtcgtgttg gggccggacg
     1141 gaagaatttc cgacatcggc aagcacgtct ccctcggccg cacagggggg gagtcggtgg
     1201 gaatcgccgc gttctccgcg ccgttcgcgc ggcgactttt cgacgtcctc gagcgccgcg
     1261 tttcccgcga gggcggtcgg gacgagtggt acgagtccgc attcgtcgag ctgatcgagg
     1321 ccggcgagct cgtttacccg gtcgacctcg gcgaactcca ggcgatcgaa gtggacacgg
     1381 ccgacgatct ccagcgcgcc cggaacctct tcgtgtagcg ctccgcgcta ccttttcggg
     1441 agctcgaggc ggacgcagca cattccgggc tccggcgcga ggacgggacg cacgccggga
     1501 aggtcgatcc cctgcacgac gccctcgatg agcgcgaggt tcatcccgca cacgagctcc
     1561 ctgtgctcac ccacgagagc gtcaaaggga cagttgcgaa atcggaccgc gccgggcgtt
     1621 gcctccggct cgtaaccctg cgaagcgagg gcgtccacca tgccggcgat cagacgcttt
     1681 ctggcgggcc gcagaccgga ctggctgcgc atttcgccgc cgatctgcgc gccgattctc
     1741 cgcgcgctct tcttgagagc accgcgggtg tcccgggcgg cgctcgtgtc gaccgccgtg
     1801 gccagaatgc gcgccgccag ttcgtaacgg cgttgcggga cgctcaactc gagctgccga
     1861 cgcgaccggc gatagacctt ggccggccgt cccgctccgg gtccggcccg ccccgataac
     1921 cggcggaacg aagcctcgag gaggccctcc tcgaccagcc gatccaggtg aaaaccggcc
     1981 aggcttcggg ataccccgac cgccttcgcc gcctcgtcgc gacccacatc ctgctgccgg
     2041 cgcacgacat agaggtaaag atcgcgtctg gccggctcgg cgagagcggc gagcccctcg
     2101 atctggcctt ccagctcccg gctttctctg gccgaactct aaaacacctg aaggcttttt
     2161 aaaaatcccg ttccgggccg cggcatctct atactagcga tgatgatctt tagaaaacct
     2221 tcttcttcga ggagagtgtc atgtcaccga tccttgccgt tctcgccctt gccctgacgg
     2281 tcccggcaac gccgtccgtc cacgccgccg actcccattc cgcggtcacc tcttcggctc
     2341 ccgtcgatcg gggcgccttc cacgatgcga
//
LOCUS       DASWAV010000261         1799 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U2665
            k141_614004, whole genome shotgun sequence.
ACCESSION   DASWAV010000261 DASWAV010000000
VERSION     DASWAV010000261.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960316
            Sequence Read Archive: SRR10260007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 1799)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1799)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:47:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,929
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1799
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_614004"
                     /isolate="SMAG_U2665"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.36444 N 132.31889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..200)
                     /locus_tag="VF958_05320"
     CDS             complement(<1..200)
                     /locus_tag="VF958_05320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9148566.1"
                     /translation="MKAVALLLVGSLLASSVLADVDEIRGEGDVCAAPASDPNEIGSA
                     ARVSPTTGALPAAPESPQEPPQ"
     gene            262..816
                     /locus_tag="VF958_05325"
     CDS             262..816
                     /locus_tag="VF958_05325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9148567.1"
                     /translation="MDPDSRRRIAEYVKPLAVGLDGVTYYGDVERLVTAARRIANGRK
                     EVDADVLYLLAVFSGQERWVSRFGHKSRTEIFLASLGIGPRTVSALWRGLARLEKEPK
                     TPEEEIVHDAMRLEDLGAYGIARRLLEGYRDQMDFGEIADSIDEAAAAPLATPEGRAL
                     ALPRIEAMREFSRRLREEYREFAG"
     gene            complement(813..1550)
                     /locus_tag="VF958_05330"
     CDS             complement(813..1550)
                     /locus_tag="VF958_05330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012241275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX9148568.1"
                     /translation="MAPPLDSKIALVTGGGIRVGRAIALALGAAGADVAVHYRGSREG
                     AESAAAEIRSQGRRAVVVSGDLASPEDCRTVVRESVAALGGLDFLVHSASNFHRATLA
                     ETDEKLWDSSMDVNARAGLLLAREAAATLRERRGRVVLVSDFLAETPARHYLAHSVSK
                     AAVEGLVRALAVELAPEVAVNGVAPGTVLPPEGTTPEEVSRLARIVPARRIGSPEDVA
                     ATVVFLCAGPAFLTGQVIRVDGGRSIV"
BASE COUNT          252 a          645 c          642 g          260 t
ORIGIN      
        1 tcctgcgggg gttcctgcgg ggactccggc gcggccggga gtgcgcccgt cgtcggggat
       61 acccgggcgg cggacccgat ctcgttcggg tccgaggcgg gcgccgcgca gacgtcgcct
      121 tccccgcgga tctcgtcgac atccgcgaga acggacgacg ccagcaggct gccgaccagc
      181 agaagggcta cggccttcac gcgtctccgg tcatgcaagt ctccggcctc gccgacgtga
      241 acggcgcgtt acgatcggac gatggatccg gactcgcgcc ggcgcatcgc cgagtacgtc
      301 aagcccctcg ccgtcggtct ggacggagtg acctactacg gcgacgtcga acggctcgtg
      361 acggccgccc gcaggattgc gaacggccgc aaggaagtcg acgcggacgt cctctatctg
      421 ctcgctgtct tctccgggca ggagaggtgg gtttcccgct ttggccacaa gagccgcacg
      481 gagatttttc tcgcgtccct cggcatcgga ccgcgaacgg tctcggcgct ctggcggggc
      541 ctggcgcgac tcgagaagga gccgaagacc ccggaggaag agatcgtcca tgacgcgatg
      601 cggctcgagg acctcggcgc ctacggcatc gcgcgccggc tgctcgaggg ataccgcgat
      661 cagatggact tcggcgagat cgcggactcg atcgacgagg ccgccgccgc gccgctcgcg
      721 acgcccgaag gccgcgctct cgccctgccg cgtatcgaag cgatgcggga gttctcgcgg
      781 cgtctccggg aagagtaccg ggagttcgcg ggctagacga tcgacctgcc tccgtcgacg
      841 cggatgacct gtccggtcag gaaggccggc cccgcgcaga ggaagacgac ggtggcggcg
      901 acgtcctcgg gcgaaccgat gcgtcgcgcg gggacgatgc gcgcgaggcg actgacctcc
      961 tcgggagtgg tgccctccgg cggcaggacc gtgccgggag cgacgccgtt cacggcgacc
     1021 tccggcgcga gctccaccgc gagggcgcgc acgagcccct cgaccgccgc tttcgacacc
     1081 gagtgggcca ggtagtggcg cgcgggggtc tccgcgagga agtccgaaac gaggacgacc
     1141 cggccccggc gctcgcgaag ggtcgcggcc gcttcccgcg cgagcaggag accggcgcgc
     1201 gcgttgacgt ccatcgagga gtcccagagc ttctcgtctg tctccgcgag cgtggcccgg
     1261 tgaaagttcg acgccgagtg gacgaggaaa tcgagtcccc cgagcgccgc caccgactcg
     1321 cgcacgaccg tccggcagtc ctccgggctc gcgaggtcgc cggagacgac gacagcgcgc
     1381 cgtccctgcg agcggatctc cgcggcggcg ctctccgctc cttcgcgcga accgcggtag
     1441 tgaacggcca cgtccgctcc ggcggcgccg agtgccaacg cgatcgcccg tcccacccgg
     1501 attccacccc cggtgacgag ggcgatcttg ctgtcgaggg gcggtgccat tcgtccgatc
     1561 gtaacctcgg ggcctccgct tcgtctctgt tgggaagaga ataggtgtgc ttcccggagg
     1621 agttccatga tccgacgccg gccgctcggc ctcgctgccg tccttctcgc gctcttcctc
     1681 gctcccgccg cccgcgcggc gaccgtctcc ggccgcgtac tcgactccgg cggcgcgccg
     1741 gtctcctgct cgcgcgggaa gcggccgcga cccttcgcga gcgccggggc cgggtcgtc
//
LOCUS       DASWAV010000262         2445 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U2665
            k141_772857, whole genome shotgun sequence.
ACCESSION   DASWAV010000262 DASWAV010000000
VERSION     DASWAV010000262.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960316
            Sequence Read Archive: SRR10260007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2445)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2445)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:47:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,929
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2445
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_772857"
                     /isolate="SMAG_U2665"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.36444 N 132.31889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..266)
                     /locus_tag="VF958_05335"
     CDS             complement(<1..266)
                     /locus_tag="VF958_05335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012307340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase"
                     /protein_id="HEX9148569.1"
                     /translation="MLRAGIVGLPNVGKSTLFNALTRTRKAEAANYPFCTIEPNVGVV
                     TVPDARLPPLARISKTTKIVPTTIEFVDIAGLVRGASQGEGLGN"
     gene            291..1343
                     /gene="cax"
                     /locus_tag="VF958_05340"
     CDS             291..1343
                     /gene="cax"
                     /locus_tag="VF958_05340"
                     /inference="COORDINATES: protein motif:HMM:TIGR00378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015368 - calcium:monoatomic cation
                     antiporter activity [Evidence IEA];
                     GO_process: GO:0006816 - calcium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="calcium/proton exchanger"
                     /protein_id="HEX9148570.1"
                     /translation="MQLALNFLLVGLPVAVLSRIAHWPEWLTFTAAALALLPLAGWIG
                     HGTEQAAARLGTGLGGLLNATFGNAAELILTIFALRRGLVDVVKASLTGAIIGNTLLI
                     VGVSAFVGGVKHGTQKFPVRPTARHAAMMILAVSAMALPAVFATVVKGEFVREEVSIG
                     VAILLLLTYGAYVFFSYFSSERERDENEDERGDAPHGGWTLRRSMLVLGASVVGTVVA
                     SEMLVYSIEPVSRSLGLSQFFIGLIVIPILGNVAENYSAIRFAAANRMSLSMSIAANS
                     STQIAVFVAPLLVLVSLFFHPMDLAFQPIELVTLFASSAIFAYISLDGETNWLEGVQL
                     LSLYLIAAVAFFFLPK"
     gene            1494..2339
                     /locus_tag="VF958_05345"
     CDS             1494..2339
                     /locus_tag="VF958_05345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9148571.1"
                     /translation="MHYLAKLRRVLVVLCIAGGLSVASFSGAGTTALASVGGLSGLAG
                     LSGKLLAIFTLPGDASLRALAGAPTAGVSLIVMKPFSSKVDGRIGNYRLGNWPYEGRT
                     PRSPNYASPKGFIEVTPDNFGTHVSEHFTVGQFLTKDQSDVWPKYLVLDRRLLDKLEL
                     TIAELDREGYAVKNFSIMSGFRTPEYNEQGVGAGGRSAVSRHQYGDASDVYPDDDRDG
                     RLDDLNRDGRVDLKDARILASAAEVVERKNPQLVGGIGIYPATSSHGPFVHIDARGTR
                     ARWNG"
BASE COUNT          391 a          803 c          768 g          483 t
ORIGIN      
        1 ttgttcccga gtccttcgcc ctgcgaagcg ccgcggacga ggccggcaat gtccacgaac
       61 tcgatcgtcg tcggcacgat cttcgtagtc ttcgagatcc gggcgagcgg cgggagccgg
      121 gcgtcgggca ccgtcacgac cccgacgttc ggctcgatcg tgcagaacgg gtagttcgcg
      181 gcctccgcct ttcgcgtgcg cgtcagcgcg ttgaagagcg tggatttccc gacgttcggc
      241 agcccgacga ttccggcgcg aagcatcggg ggagtctatg atcgcccgcg gtgcagctcg
      301 ctctgaactt cctgctcgtg gggctcccgg tcgcggtcct ctcccgcatc gcccactggc
      361 ccgagtggct gacgttcacc gctgcggcgc tggcgcttct ccctctcgcg ggttggatcg
      421 gccacggcac cgagcaggcc gccgcaagac tcggaacggg tctcggtggc ctcctgaacg
      481 cgaccttcgg caacgctgcg gagctgattc tgacgatctt cgcgttgcgc cgcgggctcg
      541 tggacgtcgt gaaggcttct ctgacagggg cgattatcgg caacacgctt ctcatcgtgg
      601 gcgtctcggc gttcgtcggc ggcgtcaaac acggcaccca gaagttcccg gtgcgaccga
      661 ccgcgcgaca cgccgcgatg atgatcctcg cggtctccgc gatggccctt cccgcggttt
      721 tcgcgaccgt cgtgaaggga gagttcgtgc gcgaggaagt gtcgatcgga gtggcgatcc
      781 tgctcctgct gacgtacggc gcttacgtct tcttctccta tttttcgtcg gagcgggagc
      841 gagacgaaaa tgaggacgag cgcggagacg ctccccacgg cggttggacc ctacgccggt
      901 cgatgctggt cctcggggcg tccgtggtcg gaaccgtcgt ggcgagcgag atgctcgtct
      961 actcgatcga gcccgtttcg cgctctctcg ggctctccca gttcttcatc ggcctgatcg
     1021 tgattccgat cctcggcaat gtggcggaga actacagcgc gatccggttt gccgcagcga
     1081 accggatgag cctctcgatg tccatcgccg cgaactcctc gacgcagatt gccgtcttcg
     1141 tggcgccgct gctcgtgctc gtcagcctgt tcttccaccc gatggacctg gccttccagc
     1201 cgatcgagct cgtcactctc ttcgcgtcct cggcgatctt cgcgtacatc tcgctcgacg
     1261 gggagaccaa ctggctcgag ggggtccagc tcctgtccct ttatctgatt gcggccgttg
     1321 ccttcttctt cctcccgaag taggcttttc tccagagacg aaaaaactcg tggtcgagct
     1381 cgccatttcc cggaatagcg ctctcaggtc acccgttctg gcttgtgctc caagacagaa
     1441 cacacagcga cgagtgctga ctccgaactc tttgaaagaa agggactaag agcttgcatt
     1501 acctggccaa gttgcgtcgc gttctcgtgg tcctgtgtat cgccggtggc ctctccgtgg
     1561 cgagcttctc cggcgccggc acgacggctc tcgcgagcgt cggaggcctc tcggggctgg
     1621 ccggtctttc gggaaaactc ctcgcgatct tcacgctgcc cggagacgct tccctgcgcg
     1681 cgctcgccgg cgctccaacc gctggagtca gcctgatcgt gatgaagccc ttttcctcga
     1741 aggtggacgg ccggatcgga aattaccgcc tgggcaactg gccctacgaa ggccgcacgc
     1801 cgcgcagccc gaactacgcc tccccgaagg gcttcatcga ggtgacgccc gacaacttcg
     1861 gaacccacgt ctcggagcac ttcaccgtcg gccagttcct gaccaaggac cagagcgacg
     1921 tgtggccgaa gtatctcgtc ctcgatcgcc gcctgctcga caagctcgag ctgacgatcg
     1981 cggagctcga tcgagagggc tatgcggtga agaatttctc gatcatgagc ggcttccgga
     2041 cccccgagta caacgaacag ggtgtcggcg cgggcggccg gtcggcggtt tcgcgtcacc
     2101 agtatggcga cgcctcggac gtctatccgg atgacgacag ggatggccgg ctcgacgacc
     2161 tcaaccgcga cggacgagtc gacctgaagg acgcgcgcat cctcgcctcc gcggccgaag
     2221 tcgtcgagag gaagaacccg cagctcgtcg gaggaatcgg gatctacccg gcgacgtcgt
     2281 cccatggtcc gttcgtgcac atcgacgccc gcgggactcg cgcgcggtgg aacggataga
     2341 gaagtccgaa gtccagagtc cgaaaagttg ccccgggaaa ccggggcttt ttctttgact
     2401 cagggacctc gaacctggga cttgggactc tcggcaactc gtcag
//
LOCUS       DASWAV010000263         2389 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U2665
            k141_772909, whole genome shotgun sequence.
ACCESSION   DASWAV010000263 DASWAV010000000
VERSION     DASWAV010000263.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960316
            Sequence Read Archive: SRR10260007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2389)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2389)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:47:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,929
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2389
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_772909"
                     /isolate="SMAG_U2665"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.36444 N 132.31889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..711
                     /locus_tag="VF958_05350"
     CDS             <1..711
                     /locus_tag="VF958_05350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9148572.1"
                     /translation="DPRLSLALGRLLDALGPALLDFSTLSLGNEADSYFADKPAELAA
                     FRRLFNGAVTYLQHKDPRLLVGVATAAPTDSPAPAVAAALHQKSPALFYLYAPFLPDA
                     SFMHRPPETLERDWKAILSAAGGRPVAFTEVSYSSAAENGSSPAKQAEFVRRMRRFVE
                     SSDRSQLLFARYVPWRDPPAESLDGHGLRPEGAGGKGAAVDRRAYFFANRGLQRFDGT
                     PKPAWRELGAGVPKRRHP"
     gene            721..1746
                     /gene="fbp"
                     /locus_tag="VF958_05355"
     CDS             721..1746
                     /gene="fbp"
                     /locus_tag="VF958_05355"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014561481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1 fructose-bisphosphatase"
                     /protein_id="HEX9148573.1"
                     /translation="MPRPPVTLDRFILEGQHAHPGATGELSLLLMRLGVAGKRIAREL
                     AIADLKHTHGDAGTENVQGEEQKKLDVRANDILVETFDYGGLVSFVASEELEVPFVYR
                     GHSDSARYLVLFDPMDGSSNIDVNGTLGTIFSIRPRRAGTEEDLLRPGSEQVAAGYIL
                     FGPAVLFVYSSGEGVNVFSLDPAIGEFVLVRRHVEMPPRGRDYAVNEGRRSGWSAADR
                     AFVDYLKENDPSTGRPYSTRYSGSMVGDVHRILLEGGIYLYPADASSGKTSGKIRMLY
                     ESHPLAFVVEQAGGRASTGKDRVLDLAPRSLHERIPVAIGSKLEVELYEQFARGEREP
                     KSARPTG"
     gene            1801..2094
                     /locus_tag="VF958_05360"
     CDS             1801..2094
                     /locus_tag="VF958_05360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008216322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HEX9148574.1"
                     /translation="MPDNKDDGIVSGGHTSVGSGTGSSESGIVSGGSSQLTSKDEHTM
                     QTYTVVKGDSLSKIAKNVYGKASLWRKIYEANQDQIKDPDLIFPGQLLRIPEA"
     gene            2106..>2389
                     /locus_tag="VF958_05365"
     CDS             2106..>2389
                     /locus_tag="VF958_05365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9148575.1"
                     /translation="MKTAYRLLTVAIVLVFASVACQKKTETAAAPAPAYEPPATAAPA
                     APMGARVERITVAKAVNSDDSPGEAASTFGKKDTVYVSMWTANAPVGTEI"
BASE COUNT          418 a          789 c          790 g          392 t
ORIGIN      
        1 gatccgcgcc tgtcactcgc gctcgggcgc ctgctcgacg cgctgggccc ggcgctgctc
       61 gatttctcga cgctctctct cggcaacgaa gccgactcct acttcgccga caagcccgcc
      121 gagctcgcag cgtttcgccg actcttcaac ggcgcagtca catacctcca gcacaaagac
      181 ccgcggctcc tcgtcggcgt cgcgaccgcc gcgccgaccg acagtcctgc ccccgccgtt
      241 gccgcggctc tccaccagaa gagcccggct cttttctatc tgtacgcgcc gttcctgccc
      301 gacgcgtcgt tcatgcaccg cccgcccgag accctcgagc gcgactggaa ggcgatcctc
      361 tctgcagcgg ggggacggcc ggtcgcgttc acggaggtga gctattcctc tgccgcggag
      421 aacggctcga gccccgcgaa gcaggcggaa ttcgtgagac gcatgcgccg cttcgtagag
      481 tcgtcggatc gctcgcaact gctcttcgcc cgttacgtgc cgtggcggga cccgccggcg
      541 gaatccctcg acggccacgg gttgcggccg gagggtgccg ggggtaaggg cgctgccgtg
      601 gatcgacgcg cttatttttt cgcaaaccgg ggcctgcagc ggttcgacgg gacgcccaag
      661 ccggcatggc gcgagctcgg cgccggggta ccgaagcgtc gacacccgta gaatccgcgc
      721 atgccgcgcc ctcccgtgac cctggaccgt ttcatcctcg agggccagca cgcccatccg
      781 ggcgcgaccg gcgagctgtc gctccttctc atgcggctcg gcgtcgcggg caagcggatc
      841 gcgcgggagc tcgcgatcgc ggacttgaag cacacgcacg gcgacgcggg aaccgagaac
      901 gtccaggggg aagagcagaa gaagctcgac gttcgcgcga acgacatcct ggtggagacg
      961 ttcgactacg gcggcctcgt cagcttcgtc gcttccgaag agctcgaagt gccatttgtc
     1021 taccgcggcc actcggactc cgcgaggtac ctggtgctgt tcgatccgat ggacggctcc
     1081 tcgaacatcg acgtcaacgg aacgctcggc acgatcttct cgatccgtcc gcgccgggcg
     1141 gggacggagg aggatctcct ccgccccggc tccgaacagg ttgcggcggg ctacatcctg
     1201 ttcggtcctg cggtcctctt cgtctattcg agcggcgagg gcgtgaacgt gttctccctc
     1261 gaccccgcga tcggcgagtt cgttctcgtt cgccggcacg tcgagatgcc gccgcgcggt
     1321 cgcgactacg cggtcaacga ggggcggcgg tccggatggt ctgcggcgga tcgggccttc
     1381 gtcgattacc tgaaggagaa cgacccgtcc accggccgcc cgtactcgac gcgctactcc
     1441 ggttcgatgg tcggcgacgt tcaccgcatt ctgctcgagg gcgggatcta cctgtatccg
     1501 gcggacgctt cgtccggcaa gacgtcgggc aagatccgaa tgctctacga gtcgcatccc
     1561 ctggccttcg tcgtcgaaca ggcgggtggc cgggcgtcga ccggaaagga ccgggtgctc
     1621 gacctcgcgc cgcgatcgct ccacgaaagg attcccgtcg cgatcggcag caagctggaa
     1681 gtcgaactgt acgagcagtt cgcgcgggga gagagagagc cgaagtccgc gcggccgacg
     1741 ggctgaaatc ggcgggcacg ggccgtgcat ttgagatgcg gagttgcgaa aggaggaggt
     1801 atgcccgaca acaaggacga cggaatagtc tcgggcggcc atacatcggt gggcagcggt
     1861 actgggtcga gcgaatccgg catcgtgtcg ggagggtcga gccagctgac gtcgaaggac
     1921 gagcacacca tgcagaccta cacggtggtg aagggcgact cgctctcgaa gattgccaag
     1981 aatgtttacg gaaaagcgag cctgtggcgg aagatctacg aagccaatca ggaccagatc
     2041 aaggatccgg atctgatctt cccgggccag cttcttcgta ttcccgaagc ttgagtggag
     2101 gagaaatgaa aaccgcatac cggttactga ccgtcgcgat cgtcctggtg ttcgcctcgg
     2161 tcgcgtgtca gaagaagacc gaaacagccg ccgccccggc tcccgcatac gagcctccgg
     2221 ccacggctgc gcccgccgcc cccatgggag cgcgcgtcga gagaatcacg gtcgcgaaag
     2281 cagtcaacag tgacgacagc ccgggcgagg cggcttcgac gttcggcaag aaggacacgg
     2341 tgtacgtctc catgtggacg gcgaacgccc ccgtcggaac cgagatcaa
//
LOCUS       DASWAV010000264        15820 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U2665
            k141_293840, whole genome shotgun sequence.
ACCESSION   DASWAV010000264 DASWAV010000000
VERSION     DASWAV010000264.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960316
            Sequence Read Archive: SRR10260007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 15820)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15820)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:47:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,929
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15820
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_293840"
                     /isolate="SMAG_U2665"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.36444 N 132.31889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..334)
                     /locus_tag="VF958_05370"
     CDS             complement(<1..334)
                     /locus_tag="VF958_05370"
                     /inference="COORDINATES: protein motif:HMM:NF024907.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VCBS repeat-containing protein"
                     /protein_id="HEX9148576.1"
                     /translation="MGSGCAFADYDRDGDWDLFVADTAGPLTLSPDEMRKTPGGCRLY
                     RNDGGVFTDVTREAGLGDLKGTFMGAAWGDYDNDGYPDLAVTSYGEIRLFHNRGDGTF
                     EDVSKKSGV"
     gene            333..527
                     /locus_tag="VF958_05375"
     CDS             333..527
                     /locus_tag="VF958_05375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9148577.1"
                     /translation="MSSGSCVERAPPEKWRKAIPEAAAMSFHRTLQSASPAIAARWTS
                     VIPSTRSPGLYGASDSPRVK"
     gene            complement(613..2661)
                     /locus_tag="VF958_05380"
     CDS             complement(613..2661)
                     /locus_tag="VF958_05380"
                     /inference="COORDINATES: protein motif:HMM:NF024827.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiheme c-type cytochrome"
                     /protein_id="HEX9148578.1"
                     /translation="MNSTAPRPPRDRAVGWKAMLGRLSAGLLAYLLLTGLPVRFLPFS
                     PLVEWSILFHTVVGLAAMAPLSLYLWRHWKAYRPDALTPSKVIGYMSLVAVVAAVGTG
                     LWATALAVFSDRVSPSVRMLHLVPSIALAILALAHLVPSWRRVPVEAGATAPFVRARR
                     GYAYATLLAPAVLLLLAFPASLLHDGPHVSGRFPPDYRMPYGAGRPFAPSLAKTESGR
                     ALAPEALAGSVACGSSGCHESIYREWSVSAHRWSALDPAFQRVQEEMARQNGPESTRY
                     CGGCHDPLSLFSGSKTVFKAKLTADAGLEEGVSCLTCHGVVKTDVQGNANYVVRSPRR
                     YLFETSKAPAARFISYFLIRAYPNQHRVSLGKRLFKTPEYCAACHKQFVDQEINNFGW
                     VQLQNQYDNWRKSKWNHPGNPRTTIECRECHMPLIAGAEPAAGDDADYNRRPDDGKHR
                     SHRFLGANQYMPALLKLPGGEEQVRLVERWLHGTYPVPEIASKWADGPVVQLDLTAPA
                     EAKRGEPLTLEAVITSRKVGHDYPTGPLDLIQSWIEVDVRDEAGRVVFSSGKPDEKRM
                     IPPGTFLFKAEPVDRYGNLIDRHNLWEMVGVRYRRSLFPGYSDVARYTFTCPASSGEP
                     APVRKIVVPSLPAGMLTVTATLHYRKLDQFLVDFLFPGKGLSAPITDLAQARARVRVI
                     "
     gene            2738..3253
                     /locus_tag="VF958_05385"
     CDS             2738..3253
                     /locus_tag="VF958_05385"
                     /inference="COORDINATES: protein motif:HMM:NF014173.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HEX9148579.1"
                     /translation="MAGSRGPRRTGDKPLRTRPPLLYSRTCQHALRVMERLAAAEVAR
                     PGALRSLAALAAAARTPLPALAQIVRRLRRAGLLQARRGPSGGVRLVRPAVQISVLDV
                     VRAVDGTGLEGRCILGFPACGDATPCPAHPVWKNARTILEARLESRSLADLARTVARK
                     NAAVAALRRRR"
     gene            3290..5029
                     /locus_tag="VF958_05390"
     CDS             3290..5029
                     /locus_tag="VF958_05390"
                     /inference="COORDINATES: protein motif:HMM:NF024907.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRTAC1 family protein"
                     /protein_id="HEX9148580.1"
                     /translation="MVSRGNSVARIFLYALLSVLCSSCRREKTPHLFRDVTEKSGVNF
                     RYRSDLVEAKLIATMGGGAALGDYDNDGSLDLFLPNSVRKSQAASNAGNCGRLYHNRG
                     DGSFEDVTRSSGIRQCAWGFGAWWVDLDNDGWLDLYITNLGSNELWRNKGNGSFAREP
                     TGRFPDDPRFSIAAAFLDANRDGLLDVFIGNYVVTSVKEESGRVLRDQKLPDEYEPPG
                     GSFLLQQADGTFENVTAEAGTAAPQGRTIGAIAFDYNRDGITDLYLANDQSPNYLFRG
                     RGDGTFEDVSAETGTDAPPDGPTAFGRRHRSGMGLASADYDGDGWPDLFITNFANEPN
                     TLYRNVEGELFEERDVQAGLAAPSVPLSAWGCNFFDWDNDGWPDLFVSNGQILPRWLY
                     WFLRAFSKKAGQYNIGEQTYRQRQHLFRNRGDGTFERIPPRVIGDLGKITRSGRGTAT
                     GDLDHDGRLDLVLAPISDSVLVFRNETPKPGHWIEILPVGTRDGDTPLHTKVSVTFEG
                     KTKTQEFTIQPSYASGSYVPLHFGLGRANRIDALGVIWPDGSVQDLPPQPLDRAYRLS
                     KKEGLLEGLRGPK"
     gene            complement(5146..6843)
                     /locus_tag="VF958_05395"
     CDS             complement(5146..6843)
                     /locus_tag="VF958_05395"
                     /inference="COORDINATES: protein motif:HMM:NF024907.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRTAC1 family protein"
                     /protein_id="HEX9148581.1"
                     /translation="MKRPAAGLALLLVLAGASAAPEAGVFRDITKASGIRMRVSGDIR
                     RLKLIPTMIGGCAFGDYDDDGRPDLYVTNSVARWGKPNGHNCGRLFQNLGGGRFADVT
                     EKAGIRVCGLGMGAFWVDLDGDGKLDLYLTNVGPNVVYWNRGDGTFEEGKDTGLEDPL
                     FSSGAGFLDYDGDGRVDVVVANYLDTTPEWEAGQPQSEFRVPEDYVGQPSHLFHNEGG
                     RKWRDVTRPAGLAIDPRETKTLGVAVLDYDGDGRPDLYFVNDRVGNRLFRNRGDGTFE
                     ETTAEAGAGVLGDHPRAGMGVAVGDPFGDGRPSLFVTNFGAEPNSFYRNLEGMLFDDA
                     GGKTGAAGIGQPYVRWGTHFADFDNDGWPDLYAVGGHLVPRLVRSLARYKTSGSAYVE
                     AGEKAYAQPSVLVHNLGGGRFEEWKNSGDFGKARMVARGTAVADIDNDGDLDLAVVDL
                     DGPVRLFENVIGSRKSWIRIEPRVSADQRIVLGTTVRVTAGGRTQTKTFWVSPSYVSG
                     SLTDLHFGLGGAAAADRIEVVWPGGDTQTFQDIAARRAYTIARSGPLAARPGPPSSR"
     gene            complement(6840..7004)
                     /locus_tag="VF958_05400"
     CDS             complement(6840..7004)
                     /locus_tag="VF958_05400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9148582.1"
                     /translation="MKSGKWYFGDVTVDPKDANVVKRRALRVRPRVPMRPGGPEGFRD
                     TLSGAPASKK"
     gene            7130..8689
                     /locus_tag="VF958_05405"
     CDS             7130..8689
                     /locus_tag="VF958_05405"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: protein motif:HMM:NF013670.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HEX9148583.1"
                     /translation="MHIKRSGSLAACLGALLVVVLAMVGPASGQSTATLVFRGQSYPI
                     ATSGADFFSVGDVAHALGLTVSRDPRSGVLTITAEGHAVVVPPAAALIPVDQRSVEIS
                     KPTRAAGGVLYAPMDLFAKAIFPLVGASGVYDSANRVWTLSERRERASAPAPPVDSAE
                     PIALDVTVVHVEPTTQVVIRESGAARFLPRLTESGMHIQWLDRPIRPPFSERRFDDPL
                     VAAIRFDGDTATVVFRESRLTANAYPLTGPDRIVVEVGPAVETSPIEPAAPPRPSIPA
                     ITIVVDPGHGGTETGAVGPGGLQEKDVTLQIARRLAAAIPKVLSSRVVLTRDSDSAIS
                     LDDRTSVANHEKANLFLSLHANSSRATGAHGSETYYLSLESSDRISQEVARRENEVSA
                     APPAPGAPAQNPDLDFILWDLAQSAHINESSRLAEAIQTQLNVVSKTENRGIKQAPFR
                     VLSGAAMPAVLVEIGFISHAEEEKQLRSATFQESVAGAIARAVGEFFARRQTAAPVVG
                     PTPAATPSPAR"
     gene            8686..9291
                     /locus_tag="VF958_05410"
     CDS             8686..9291
                     /locus_tag="VF958_05410"
                     /inference="COORDINATES: protein motif:HMM:NF022112.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GerMN domain-containing protein"
                     /protein_id="HEX9148584.1"
                     /translation="MSRRAAALLLLALAVLAAGLILVVRRPAPSAAVRRRLPFLRVSP
                     APGSAITPLPGGGTTREETVRVTLFFADAAEGRLHREERDIARPAGPGAYLRALFGEL
                     ARGPSTTGLVAALPPRIQLRNGFLMPDGLVVLDLAVDSSLSFGSAEELSIVASLVNTV
                     LQNVANTQRVRILVNGEPAESLGGHVDLTRPLTFIESEVAS"
     gene            9297..10046
                     /gene="rph"
                     /locus_tag="VF958_05415"
     CDS             9297..10046
                     /gene="rph"
                     /locus_tag="VF958_05415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006116652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004549 - tRNA-specific ribonuclease
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HEX9148585.1"
                     /translation="MTPRPDGRKPTEMRPVSIDTDAIRHAEGSALIALGDTRVLCVAS
                     VEDRVPPFLKGTGSGWVTAEYGMLPRATHTRSAREAATGRQGGRTHEIQRLIGRSLRS
                     VVELRGLGERTVTLDCDVLQADGGTRCASITGACVALAIALAKLVRERMIPRWPLRET
                     VAAVSVGICAGQAVLDLAYVEDSEADADFNIVGTESGLFVEVQGTAERRPFSEEKLVE
                     LLGLARKGLKKLAAAQSDALRPHLSGVETGD"
     gene            complement(10043..12241)
                     /locus_tag="VF958_05420"
     CDS             complement(10043..12241)
                     /locus_tag="VF958_05420"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX9148586.1"
                     /translation="MQKGGRRRPAASGADTRAVTATPSPEPRRTRRILVGLLLGALTL
                     AVFWPLLASEFVFLDDPEYVFENRGVISGLTWPGVRWAFTTGHAANWHPLTWLSHMAD
                     VSLFGLKPAGHHATNLLLHTIAAILVFLAFQGLSGSVGRSAWIAALFAVHPAHVESVA
                     WISERKDVLSAVFWFATILAYAVWVRRRGAGRYALLLLLFAAGLMSKPMVVTLPVVLL
                     LLDYWPLGRFAGNDRLEPSVRIFGKRIVEKLPLFLLAMASSVLTFLVQRAGGAVRPLE
                     AYPLGVRLGNAILAYVRYLRVAFWPTDLAVFYPHPGKSITLTLVAAGALLLFAITAAV
                     VAMRRSAPFLLVGWTWFVVTLLPVIGLVQVGNQATADRYTYIPYVGLFLAIAWGGAWI
                     ADRFGRGRSVLRAVAIASIMALAVTAALQARVWKDSETLLLHAIRVTRNNDVAQNNLG
                     NYYNTIGRPADALPHLQEALRLRPKDPAVLVNIGHSLFLLKRFDEAAAKFSEALQRDP
                     SDDVAMNNLARVRYLQGDVPESIRLYRAALVAQPDWIDSRKRLVLALLLEEQNAAALL
                     ELERVVSRFPKDEESRQLLDQLSVYVRDPQDASARRLRGVLAAEHRTLGVALMERKRN
                     GEAATQLDRALALFPDDPLTHLNRGVLFSESHRLDEAAAEFRETLRLDPRSALAHTDL
                     GYVFFLQGLREEAIAQHREALRIQPDFPLARNNLAIAEKESPVPARPGRD"
     gene            complement(12270..12665)
                     /locus_tag="VF958_05425"
     CDS             complement(12270..12665)
                     /locus_tag="VF958_05425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9148587.1"
                     /translation="MARDEGAKTFPAISVRQPNAGRIIAGELPHDIRSRQTRFRGWIL
                     LHASSTFRRGEVTKVDGAGPELGKLLGLVKLADCVADGEGWKYVWTNPRRFRTPVACR
                     GHYSIPFYVPAKLVAGTPAAKVTAGKLEG"
     gene            12785..13816
                     /locus_tag="VF958_05430"
     CDS             12785..13816
                     /locus_tag="VF958_05430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014560152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9148588.1"
                     /translation="MKKIGVLFGQERTFPYALVDRINRAGGPGVTAEPMKVGGVSLET
                     PRKYDLVLDRISQDIPFYRAVLKKLAFDGTIVVNDPFWWSADDKCFNNVIAKSIGVAV
                     PKTVLLPSNEHPPDTTSASMSNLVFPLDWDEIFSHVGFPAYFKPHSGGGWKNVYRVTN
                     PDEFFAAYRSSGQNVMILQEEIVFQDYFRCYVLGAKYVHLMRYDPRQPHENRYVHGGP
                     PIEPALEERLREDCLKLAGALGYEFDTVELAVRDGIPYAIDFLNPAPDADAASIGPDN
                     FEWVVETSARWLIERVNEGRKPSRAWEWEKAREAVASTEPDTDRPKKPRSSRRPRRTR
                     SPEEAPASE"
     gene            13813..14961
                     /locus_tag="VF958_05435"
     CDS             13813..14961
                     /locus_tag="VF958_05435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011685651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004357 - glutamate-cysteine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0042398 - cellular modified amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylate-amine ligase"
                     /protein_id="HEX9148589.1"
                     /translation="MTVERPAFTLGVEEEFQIIDPETRELRSHVQEMLDEGVRVLKER
                     VKPEMHQSVMEIGTGICKDAGEVRRDVVELRSEIVRLAERSGLRVAAAGTHPFSSWAD
                     QLIYPNERYAAIVEEMQLVARANLIFGLHVHVGISDRALALQIMNEARYFLPHFLALS
                     TNSPFWLGRNTGLKSYRTKVFEKFPRTNIPEVFETPAEFDDYVRILVKTNCIDNGKKI
                     WWDVRPHPFFDTIEFRVCDVPMRVDETVAIAALIQAICAKLFQLREKNLGWRTYRRAF
                     LLENKWRASRYGVEGKLIDFGKEIEVPFRDLAEEMLEFVDDVVDELGSRREVESIRWI
                     LENGSGADRQLAVYRESGEDLRKVVDYICEETRHGLDVAQSGSAAAIG"
     gene            14958..>15820
                     /locus_tag="VF958_05440"
     CDS             14958..>15820
                     /locus_tag="VF958_05440"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: protein motif:HMM:NF014164.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HEX9148590.1"
                     /translation="MTIERNGNGNGYDLVPGARNAIRTCLRVAPEEHVAILTDRETEP
                     VAASLAAEIREVGAPYELLVLEDYGDRPMFELPQPVREAFERSAVSIYAGQPAPGELA
                     YRREMTAIVNRRKIRHAHMVSISPRIMAEGMRADFEEVNAISTRLRDRAVKAERIRAR
                     SRAGSDLEATFDPSVKWLKTSGLITPDKWANLPGGEVFTAPARVDGVYVVDGVLGDYL
                     CARYGDLEETPLTVWIEDSRVARAECARSDVRDDFLEYIDTAENSNRVGEFAMGTNIA
                     VDSIIGNILQD"
BASE COUNT         2616 a         5359 c         5363 g         2482 t
ORIGIN      
        1 ccacgccgga cttcttcgag acgtcctcga aggtgccgtc gccccggttg tggaagaggc
       61 ggatctcgcc gtacgaggtg acggcgaggt cggggtagcc gtcgttgtcg taatcgcccc
      121 acgcggcgcc catgaaggta cccttcagat cgcccagccc cgcctcccgc gtcacgtccg
      181 tgaagacgcc gccgtcgttg cggtagaggc ggcagccgcc gggagttttt cgcatctcgt
      241 ccggcgagag cgtgagcggg cccgccgtgt cggcgacgaa gaggtcccag tcgccgtctc
      301 ggtcgtagtc ggcgaaggcg cagccggagc ccatgtcctc cgggagttgc gtcgagcgcg
      361 cgccgcccga gaagtggcga aaggcgatcc ccgaggccgc ggcgatgtcc ttccaccgga
      421 cgctccagtc ggcgtccccc gcgatcgcgg cgcggtggac gtccgtgatc ccctcgaccc
      481 gctctcccgg cttgtacggc gcgtcggact cgccgcgcgt gaagtagacg gcggcgcaga
      541 tgacgagcgc cgcggcgccc ccggcggcga gcgcccgccg gtgcgtccag cgccgcagga
      601 cgttggagcc gctcagatga cgcgcaccct ggcccgggcc tgcgcgaggt cggtgatcgg
      661 cgcggagagc cccttgcccg ggaagaggaa gtcgaccagg aactggtcca gcttgcggta
      721 gtggagcgtc gcggtgaccg tcagcatccc ggctggcagt gaaggcacga cgatcttacg
      781 aaccggcgcc ggctcgccag acgaggcggg acaggtgaag gtgtaacgcg cgacgtccga
      841 atagccggga aacaggctgc gccggtagcg cacgcccacc atctcccaga ggttgtgccg
      901 gtcgatcagg ttcccgtagc ggtcgaccgg ctccgccttg aagagaaagg ttccgggcgg
      961 gatcatccgc ttttcgtcgg gcttgcccga ggagaacacg acgcgccccg cctcgtcgcg
     1021 cacgtcgacc tcgatccacg actgaatgag gtcgagcggc cccgtcggat agtcgtgccc
     1081 caccttgcga gacgtgatga ctgcctcgag cgtcagcggc tcgcctcgct tcgcctccgc
     1141 cggcgccgtg agatcgagct gcacgaccgg cccgtccgcc cacttgctcg caatctcggg
     1201 aaccgggtag gtgccgtgca gccaccgctc gaccagacgc acctgctcct cccctcccgg
     1261 cagcttcaag agcgccggca tgtactggtt ggcgccgagg aagcggtggc tccggtgttt
     1321 gccgtcgtcg ggccgccggt tgtagtcggc gtcgtcgccc gcggccggct ccgccccggc
     1381 gatgagcggc atgtggcact cgcggcactc gatcgtcgtc cgcggattgc ccgggtggtt
     1441 ccacttgctc ttgcgccagt tgtcgtactg gttctgcagc tgcacccagc cgaagttgtt
     1501 gatctcctgg tcgacgaact gcttgtggca ggccgcgcag tactcgggcg tcttgaagag
     1561 ccgcttcccg agcgaaacgc ggtgctggtt cgggtaggcg cggatcagga aatacgagat
     1621 gaaccgggcg gccggcgcct ttgacgtctc gaagaggtag cggcgcggcg agcgcaccac
     1681 gtagttagcg ttgccctgaa cgtcggtctt gacgaccccg tggcacgtca ggcacgagac
     1741 cccctcctcg agccccgcgt cagccgtaag cttcgccttg aagaccgtct tgctgccgga
     1801 gaagagcgag agcgggtcgt gacagccgcc gcagtagcgg gtcgactcgg ggccgttctg
     1861 ccgcgccatc tcttcctgaa cgcgctggaa ggcagggtcg agagccgacc agcggtgcgc
     1921 ggaaacggac cattcgcggt agatcgactc gtggcatccg gaggagccgc aggcaaccga
     1981 tccggcgagc gcctccggcg ccagggcccg tcccgactcg gtcttcgcga ggctcggcgc
     2041 gaaggggcgg ccggcgccgt acggcatccg gtagtccggc gggaaacgcc cggagacgtg
     2101 cgggccgtcg tgcaggaggc tcgccggaaa ggcgagaagc agcagcaccg cgggagcgag
     2161 cagggttgca taggcgtagc cgcgccgcgc ccgcacgaac ggggctgtcg cgcccgcctc
     2221 gaccggcacg cgccgccacg acggaacgag atgcgccagg gcgaggatgg cgagcgcgat
     2281 ggacgggacg aggtggagca tccgcacgga aggcgatacc cggtcggaaa agacagcgag
     2341 cgccgtcgcc cacaggccgg tgccgaccgc ggccacgacg gcgacgagcg acatgtagcc
     2401 gatcactttc gatggggtca gggcgtccgg ccggtaggct ttccagtgcc gccagaggta
     2461 gagggaaagc ggcgccatcg ccgcgagccc cacgacggtg tgaaagagga tgctccactc
     2521 gacgagcggc gagaacggca gaaagcgcac cggcaggccg gtcaggagca ggtatgcgag
     2581 gagtccggcg gagaggcgac cgagcatcgc tttccagccg acggcgcgat cgcggggcgg
     2641 acgcggcgcg gtcgaattca tgagtttccc ctcaacaatt gggacactgt agcggcgctg
     2701 ccgcgccggc gtcaaacgcg tgctaacttt gcggacgatg gccgggtcgc gcggaccacg
     2761 cagaacgggc gacaagcctc tccgaacgcg gccgccgctg ctctactccc ggacctgcca
     2821 gcacgcgctg cgggtaatgg agcggctcgc ggccgccgaa gtcgcgcgcc ccggcgctct
     2881 ccgcagcctt gccgctctgg ccgcggcggc ccgcacgcct ctgcccgcgc tggcgcagat
     2941 cgtccgtcgc ctgcggcgcg ccggactgct gcaggcgcgc cgcgggcctt ccggaggcgt
     3001 gcggctggtc cggcccgccg tgcagatctc ggtcctcgat gtcgtccgtg cggtcgatgg
     3061 cacgggactg gagggtcgct gcatcctggg atttccggcc tgcggggacg cgactccctg
     3121 ccccgcgcac ccggtctgga agaacgcacg caccatactc gaggcgcgcc tggaaagccg
     3181 ctccctcgcc gacctcgcgc gcacggtggc tcggaagaac gccgcagtgg cggcgcttcg
     3241 tcggcgacgc tagccaggct caagctccgg tggactagcc gggatcgtca tggtcagccg
     3301 gggcaactcc gtggctcgaa tcttcctcta cgctctcctg agcgtgttgt gctcctcctg
     3361 ccgccgggag aagacgcctc acctttttcg ggacgtcacc gagaagtccg gagtgaactt
     3421 tcgctaccgg tcggaccttg tcgaggcaaa gctcatcgcg acgatggggg gcggggccgc
     3481 gctcggcgac tatgacaacg acgggagcct cgaccttttc cttccgaatt ccgtccgaaa
     3541 gtcgcaagcg gcctcgaatg ccggtaactg cgggcgcctg taccacaacc gtggagacgg
     3601 ctccttcgaa gacgtgaccc ggtcctccgg gatccgccag tgcgcttggg gattcggcgc
     3661 ctggtgggtg gacctcgaca acgacggctg gctcgatctc tacatcacga acctcggatc
     3721 caacgagctc tggcgaaaca aggggaacgg ctccttcgcc cgcgagccga cgggccgctt
     3781 tccggacgac ccgcgcttct cgattgccgc cgccttcctc gatgccaatc gcgacggtct
     3841 gctcgacgtc ttcatcggga actacgtcgt cacctcggtc aaggaggaat cggggcgggt
     3901 gcttcgcgac cagaaacttc ccgacgagta cgagcctccc ggcgggtcct tccttctcca
     3961 gcaagccgac gggactttcg aaaacgtgac ggcggaggcc ggtacggcgg cgccgcaggg
     4021 acgcacgatc ggagcgatcg ccttcgacta caaccgagac gggatcaccg acctctacct
     4081 cgccaacgac cagtccccaa actacctgtt ccgcggccgg ggggatggca cgttcgagga
     4141 tgtctcggcc gaaacgggaa ccgatgcgcc tcccgatggc ccgacggcct tcggccggag
     4201 acatcggtcg gggatgggac tcgcgagcgc cgattacgac ggggacggtt ggcccgacct
     4261 cttcatcacc aatttcgcca acgagcccaa taccctctac cgaaacgtcg agggagagct
     4321 attcgaggag agggatgtgc aggcgggtct tgccgctcca tcggtccctc tctcggcgtg
     4381 gggttgcaac ttcttcgact gggacaacga cggctggccg gatcttttcg tctcgaacgg
     4441 tcagattctt ccgcgctggc tctattggtt cctgcgcgcc ttttcgaaaa aggcgggcca
     4501 atacaacatc ggcgagcaga cgtaccggca acggcaacat ttgtttcgca acagggggga
     4561 tgggacgttt gaacggatcc ctccacgggt gataggcgac ctggggaaga tcacgaggtc
     4621 cggccggggg acagcgaccg gagacctcga tcacgacggc cgcctcgatc tcgtactggc
     4681 gccgatttcc gattccgttc tggttttccg taacgaaacg ccgaagccgg ggcattggat
     4741 cgaaatcctt cccgtcggca cgcgagacgg ggatacgccc ctccacacca aagtcagtgt
     4801 gacgttcgag gggaagacga agacgcagga atttacgatc cagccgtctt atgcctcggg
     4861 ctcttacgtg ccgctgcact tcggcctcgg ccgcgccaac cggatcgacg cgctcggcgt
     4921 gatctggccg gacggatcgg tgcaggacct gcccccgcag ccgctcgatc gcgcctatcg
     4981 gctatcgaaa aaggaagggc tcttggaggg cctcagggga ccgaaatgag cggctcaaca
     5041 cggtcctggt gggagtgcag aaactactct tcacaaactc gaagacttcc cgggaactga
     5101 gccggtctcc cgagggtaac ccgctgcacc gcctcagagt cgcctctagc gggaggacgg
     5161 cggaccagga cgtgcagcga gcggcccgct ccgcgcaatc gtgtaagcgc gccgggcggc
     5221 aatgtcctgg aacgtctgcg tgtcgccgcc gggccagacg acctcgatac ggtcggcggc
     5281 cgcagcgccg cccaggccga aatggaggtc cgtaagggag cccgacacat aggacggcga
     5341 gacccagaag gtcttcgtct gggtgcgccc gccggccgtc acgcgcaccg tcgtgccgag
     5401 gacgatgcgc tgatcggccg agacccgcgg ctcgatgcgg atccagctct tcctgcttcc
     5461 gattacgttc tcgaagaggc ggacggggcc gtcgagatcc acgaccgcga ggtcgaggtc
     5521 gccgtcgttg tcgatgtcgg cgaccgccgt gccccgcgcc accatccgcg ctttgccgaa
     5581 gtctccgctg ttcttccact cttcgaagcg gccgccgccc aggttgtgca cgaggacgga
     5641 gggctgcgcg taggccttct cgcccgcctc gacgtacgcc gatccgctgg tcttgtacct
     5701 tgcgagcgac cgcacgaggc gcggcacgag atgcccgccg acggcgtaga ggtcgggcca
     5761 gccgtcgttg tcgaagtcgg cgaagtgggt gccccagcgc acgtaaggct ggccgatgcc
     5821 ggcggcgccc gtcttcccgc cggcgtcgtc gaagagcatc ccttccaggt tgcggtagaa
     5881 actgttcggc tcggcgccga agttcgtgac gaagaggctc ggccgcccgt cgccaaacgg
     5941 gtcccccacg gccacgccca tgcccgcacg cgggtggtcg ccgagtactc ccgccccggc
     6001 ctccgccgtc gtctcctcga acgtcccgtc gccccggttg cggaagaggc ggttgccgac
     6061 ccggtcgttg acgaagtaga ggtcgggcct tccgtcgccg tcgtagtcga ggacggcaac
     6121 gccgagcgtc ttggtctcgc gcgggtcgat tgcgagaccc gccggccggg tcacgtcgcg
     6181 ccacttgcgc ccgccctcgt tgtggaagag gtgactcggc tgtccgacgt agtcctccgg
     6241 cacgcgaaac tcggactgcg gctggcccgc ttcccattcg ggcgtcgtgt cgaggtagtt
     6301 cgccacgacg acgtccacgc ggccgtcgcc gtcgtagtcg aggaagcccg cgccgctcga
     6361 gaagagcggg tcctcgaggc cggtatcctt cccctcctcg aacgtcccgt cgccgcggtt
     6421 ccagtacacg acgttcggcc ccacgttcgt cagatagagg tcgagcttcc cgtcgccgtc
     6481 aaggtccacc cagaaggctc ccatcccgag gccgcagacg cggatccccg ccttctccgt
     6541 gacgtccgcg aaccgcccgc cgccgaggtt ctggaagagc cggccgcagt tgtgcccgtt
     6601 cggcttgccc cagcgggcga cgctattggt cacatagagg tcgggcctgc cgtcgtcgtc
     6661 gtaatcgccg aaggcgcagc cgccgatcat cgtcgggatg agcttcagcc gccgtatgtc
     6721 cccgctcacg cgcatccgga tgcccgacgc ctttgtgatg tcgcgaaaga cgccggcctc
     6781 gggcgccgcg gaggcgcctg cgagaacgag gaggagggcg agaccggccg ccgggcgctt
     6841 cacttcttcg aggcaggcgc gccactcagg gtgtcgcgga agccttcggg accgcccggc
     6901 cgcataggta ctcgcggacg aacgcgcagc gctcgtcgct tgacgacgtt ggcgtccttc
     6961 ggatcgaccg tcacgtcgcc gaagtaccat ttcccgctct tcaagtcccc gcgccattct
     7021 gcggatgccc tgcgtttcct tcctcctcgc tttcgagccg ggtcctcttc gagattcgag
     7081 gggccgggac gccgattcac tcggctacac tagtcgcgta ggccttttca tgcacatcaa
     7141 acggtcgggt tcgctcgcgg cgtgcctcgg ggcgctcctc gtcgtcgtcc tggcgatggt
     7201 cggccccgcc tcagggcagt cgacggcgac actcgtcttt cgcgggcagt cctatccaat
     7261 tgcgacgtcc ggcgccgact tcttcagcgt cggcgacgtc gcgcatgctc tcggccttac
     7321 ggtctcccgc gacccgcgga gcggcgttct cacgatcacg gcggagggcc atgcggtcgt
     7381 cgtcccgccg gccgcggcgc tgataccggt cgatcagcgg agcgtcgaga tctcgaaacc
     7441 gacgcgcgcg gctggcggcg tgctctacgc gccgatggac ctcttcgcga aggcgatctt
     7501 tccgctggtc ggcgcgagcg gcgtgtacga ttccgcaaac cgcgtctgga cgttgagtga
     7561 gcgccgcgaa cgggcctccg cgcccgcgcc cccggtcgac tccgccgagc cgatcgctct
     7621 cgacgtcacc gtcgtgcacg tggagccgac gacgcaggtc gtgatccgcg aatcgggcgc
     7681 cgcgaggttc ctcccgcgac tgaccgagtc cgggatgcat atccagtggc tcgaccgccc
     7741 gatcaggccg cccttttccg agcgccgttt cgacgacccc ttggtcgccg cgatccggtt
     7801 cgacggcgac acggccaccg tcgttttccg cgagtcgaga ttgaccgcga acgcgtatcc
     7861 gctcacgggc cccgaccgga tcgtcgtcga ggtcggcccg gccgtcgaga cttcgccaat
     7921 cgagccggcc gctccccctc gcccctcgat tcccgcgatc acgatcgtcg tcgatccggg
     7981 ccacggcggc accgagacgg gcgcggtcgg tccgggaggc ctccaggaga aggacgtcac
     8041 gcttcagatc gcgcgccgtc tcgcggccgc catcccgaaa gtcctttcga gtcgcgtcgt
     8101 tctcacccgt gactccgaca gcgcgatctc actcgacgac cgcacgtcgg tcgccaacca
     8161 cgagaaggcg aacctcttcc tctcgttgca cgcgaactcc tcgcgcgcga cgggcgcgca
     8221 cggctccgag acgtactacc tctcgctcga gagctccgac cggatctccc aggaggtggc
     8281 gcggcgtgag aacgaggtct ccgcggcccc tccggcgccg ggagctccgg cccagaaccc
     8341 cgacctcgat ttcattctct gggatctcgc ccagagcgcc cacatcaacg agtcgagccg
     8401 gctcgccgag gcgatccaga cgcagctgaa cgtcgtatcg aagaccgaga atcgcggcat
     8461 caaacaggcg ccgttccggg tcctttcggg tgcggcgatg cccgcagtcc tcgtcgagat
     8521 cggcttcata tcgcacgccg aggaggaaaa gcagctccgg tccgcgacct tccaggaaag
     8581 cgtggcgggc gccatcgcaa gagctgtcgg agagttcttc gcccgccggc agacggcggc
     8641 gcctgtggtc gggccgactc ccgccgcgac cccctcgccg gcgcgatgag ccgccgcgcc
     8701 gcggcgctcc tcctcctcgc gctcgccgtc ctggccgccg gattgattct cgtcgtccgg
     8761 cggccggccc cctccgcagc ggtgcgccgc cggcttccgt tcctccgcgt cagcccggcc
     8821 cccggcagcg cgatcacgcc gttgccgggg ggaggaacga cacgggagga gaccgttcgc
     8881 gtgacgctct ttttcgccga cgcggcggag ggacgactcc accgtgagga gcgcgacatc
     8941 gcccgtcccg ccggtcccgg ggcctacctc cgcgctctct tcggggagct cgcgcgcggc
     9001 ccgtcgacga ccggtctcgt cgcggcgctc ccgccgcgga tccagcttcg caacgggttt
     9061 ctgatgccgg acggtctcgt cgtgctcgat ctggccgtag actcgagcct ttccttcggg
     9121 tccgccgagg agctttcgat cgtcgcttcg ctcgtcaata ccgtgcttca gaacgtcgcg
     9181 aacacgcaac gcgtgaggat tctcgtcaac ggcgagcccg cggaatcgct cggcgggcac
     9241 gtggacctga cccgccctct cacgttcatc gagagcgagg tcgcttcgtg acgcgcgtga
     9301 caccgcgccc ggacggtcgc aagccgaccg agatgcgccc ggtgtcgatc gacaccgatg
     9361 ccatccggca cgcggagggc tcggcgctga tcgcgctcgg cgacacgcgc gtcctctgtg
     9421 tcgcctccgt cgaagaccgc gtgccgccgt tcctgaaggg aaccggcagc ggctgggtca
     9481 cggcggagta cggcatgctc ccgcgtgcga cgcacacccg ctcggcgcgc gaagcggcga
     9541 ccggccgcca gggcggacgc acgcacgaaa tccagcggct catcgggagg tcccttcgca
     9601 gcgtcgtcga gctgcgcggg ctgggcgaac ggaccgtcac cctcgactgc gacgtcctcc
     9661 aggcagacgg cggaacgcgc tgcgcctcga tcaccggagc gtgcgtcgcg ctcgcgatcg
     9721 ccctcgcgaa gctcgtgcgc gaacggatga tcccgcgatg gccgctccgt gagaccgtcg
     9781 cggccgtttc ggtggggatc tgcgccgggc aggcggtgct cgatctcgct tacgtggagg
     9841 actcggaggc ggacgcggac ttcaacatcg tcgggacgga gtccggcctg ttcgtcgagg
     9901 tgcagggcac ggccgagcgg cgtccattct ccgaggagaa gttggtcgag ttgctcgggc
     9961 tcgcgcggaa ggggctgaag aagctcgccg ccgcccagag cgacgcgctc cggccccatc
    10021 tctccggcgt cgaaacgggc gactagtccc gccccgggcg ggccggaacc ggactctctt
    10081 tctccgcgat cgcgaggttg ttgcgcgcga gggggaagtc gggctggatg cggagagcct
    10141 cgcgatgctg ggcgatcgcc tcctcccgca gcccctggag aaagaacacg taccccaggt
    10201 cggtgtgcgc gagcgccgag cgcgggtcga gccgcaacgt ctcccgaaat tcggcggcag
    10261 cctcgtcgag tcggtgcgac tcggagaaga ggactccccg gttcaggtgc gtgagcggat
    10321 cgtcgggaaa gagcgccagc gcgcgatcga gctgcgtggc cgcctccccg ttgcgcttcc
    10381 tttccatgag agcgacgccg agggtccgat gctccgccgc aaggaccccg cggaggcgac
    10441 gggcggatgc atcctgaggg tcacgaacat aaacgctcag ctggtcgagc aattgccgcg
    10501 attcctcatc cttcggaaaa cgtgacacga ctcgctcgag ctcgagaagc gcggcggcgt
    10561 tctgctcttc gagaagcagc gcgaggacga gtctcttgcg cgagtcgatc caatcgggct
    10621 gcgccactag ggccgcacga tagagccgga tcgattcggg cacgtcgccc tgaaggtatc
    10681 gaacccgggc gaggttgttc atcgcgacgt catcggaagg gtcgcgctga agggcttccg
    10741 agaatttcgc ggccgcctcg tcgaagcgct tcaggaggaa gagggagtga ccgatattga
    10801 cgagcaccgc cggatccttc ggacgaagcc ggagggcctc ctgcaggtgc ggcaacgcat
    10861 ccgcaggtcg cccgatggtg ttgtagtagt tgcccaggtt gttctgtgcg acgtcgttgt
    10921 ttcgggtcac gcggatggcg tgcaggagca gcgtctcgct gtccttccag acccgggcct
    10981 ggagcgcggc cgtcaccgcg agagccatga tggaggcgat ggcgaccgcg cgcaggacgc
    11041 tccgcccccg gccgaagcga tcggcgatcc atgctccgcc ccacgcgatc gcgagaaaca
    11101 acccaacgta gggaatgtag gtgtagcggt ccgcggtcgc ctggttgccg acctgcacga
    11161 gaccgatgac ggggagcagc gtcacgacga accaggtcca gccgacgagg aggaacggag
    11221 cactccgccg catcgcgacg acggccgcgg tgatcgcgaa aaggagcagc gcccccgcgg
    11281 cgaccaacgt gagagtgatg gacttgccgg ggtggggata gaaaaccgcg agatccgtcg
    11341 gccagaacgc cacccggagg tatcgcacgt acgcgaggat cgcgtttccc agccgcacgc
    11401 cgagcggata ggcctcgagg ggacggaccg cccccccggc tcgctggacg aggaacgtca
    11461 ggaccgaaga ggccatcgcc aggagaaaga gcggcagctt ctcgacgatc cgctttccga
    11521 agattcggac ggacggctcg agccggtcgt tcccggcaaa tcggccgagc ggccagtaat
    11581 cgaggagaag caacacgacc ggcagcgtca cgaccatcgg cttcgacatc aggcccgcag
    11641 caaacagaag caggaggagc gcgtaacgcc cggcgccgcg tcgccggacc cagacggcgt
    11701 acgcgaggat cgtggcgaac cagaagaccg cggagagcac gtccttccgc tccgagatcc
    11761 aggcgacgga ttccacgtga gccgggtgaa cggcgaaaag tgcggcgatc caggcgctcc
    11821 ggccgacgct cccggacaga ccctggaagg cgagaaaaac gaggatcgcg gcaatcgtgt
    11881 ggaggagcag attcgtcgcg tggtgtcccg cgggtttcag accgaagagc gagacgtccg
    11941 ccatgtgcga gagccaggtg agcggatgcc agttcgccgc gtggccggtc gtgaaggccc
    12001 agcggactcc gggccaggtg agtccggaga tcaccccacg gttctcgaag acgtactcgg
    12061 ggtcatcgag aaacacgaac tcgctcgcga gaaggggcca gaagacggcc agggtgagcg
    12121 caccgagaag caggccgacg aggattcgtc gggtccggcg gggttcgggg gatggggtcg
    12181 cggtaaccgc ccgagtgtct gcacccgacg ccgcagggcg acggcgtccc cctttctgca
    12241 tcgatgcgat tatcgcgtga ggccgatatt cagccttcca gcttccctgc cgtgaccttc
    12301 gccgccgggg tccccgcgac gagcttcgcc ggaacgtaga acgggatcga gtagtggccg
    12361 cggcacgcga ccggggtccg gaaccgccgc gggttcgtcc agacgtattt ccacccttcg
    12421 ccgtccgcga cgcagtcggc gagcttcacg agtccgagga gtttcccgag ctcggggccg
    12481 gcgccgtcca ccttcgtcac ctcgccgcgt cggaaggtac tcgacgcgtg aaggaggatc
    12541 cagccccgga accgggtttg gcgcgaccgg atgtcgtgcg gtaactcgcc ggcgatgatc
    12601 cggccggcgt tgggctgccg gacggagatc gccgggaacg ttttcgcccc ttcgtcgcga
    12661 gccatcttct gcaagagtgt tgtcgcgagg gcgagaggcc ggtggcaacg gctccccggc
    12721 cctgacataa ttgcccgttt cggcggtgcg gcaagacgcg gccgacagga ggaagtcccg
    12781 tctcatgaag aaaatcggtg ttcttttcgg ccaggagcgc accttccctt acgcgctcgt
    12841 cgaccgcatc aaccgcgcgg gcggccccgg cgtcacggcg gagccgatga aggtcggcgg
    12901 cgtgagcctg gaaacgccgc gcaagtacga cctcgtcctc gaccggatca gccaggacat
    12961 ccccttctac cgcgccgtgc tgaagaagct cgccttcgac ggaacgatcg tcgtcaacga
    13021 ccccttctgg tggtccgccg acgacaagtg cttcaacaac gtgatcgcga agagcatcgg
    13081 ggtcgcagtg ccgaagaccg tgctcctgcc ctccaacgag cacccgcccg acacgacgtc
    13141 cgcgtcgatg tcgaacctcg tcttcccgct cgactgggac gagatctttt cccacgtcgg
    13201 tttcccggcg tatttcaagc cgcactcggg cggcggatgg aagaacgttt accgcgtcac
    13261 gaatcccgac gagttcttcg cggcgtaccg ctcgtccggg cagaacgtga tgatcctgca
    13321 ggaggaaatc gtcttccagg actacttccg ttgctacgtc ctcggagcga agtacgtcca
    13381 cctcatgcga tacgacccgc gccagccgca cgagaaccgg tacgtgcacg gcgggccccc
    13441 gatcgagccg gcgctcgaag agcgcctccg cgaggactgc ctgaagctcg ccggcgccct
    13501 cggctacgag ttcgacacgg tcgagctcgc ggtgcgcgac gggatcccct atgcgatcga
    13561 cttcctgaac cccgcgccgg acgctgacgc cgcctcgatc ggcccggaca acttcgagtg
    13621 ggttgtggag acgtcggcga ggtggctcat cgagcgcgtg aacgaaggtc gcaagccgtc
    13681 gcgcgcctgg gagtgggaga aggcccgcga agcggtcgca tcgaccgagc ctgacaccga
    13741 caggccgaag aagccccgct cgtcccgccg tccgcggcgc acacgctcgc ccgaggaggc
    13801 gccggcgtcc gaatgacggt cgagcgtccc gcattcaccc tcggcgtcga ggaggagttc
    13861 cagatcatcg atccggagac gcgcgagctg cgttcgcacg ttcaggagat gctcgacgag
    13921 ggtgttcgcg tcttgaaaga gcgcgtcaag cccgagatgc accaatccgt catggagatc
    13981 ggcaccggga tctgcaaaga cgccggcgaa gtccgccggg acgtcgtcga gctgcgctcc
    14041 gagatcgtcc gtctcgcgga gaggagcgga ctcagggtcg ccgcggcggg aacgcacccg
    14101 ttctccagct gggcggacca gctgatctac ccgaacgagc gatacgccgc gatcgtcgag
    14161 gaaatgcagc tcgtggcgcg ggcgaacctc atcttcgggc ttcacgtcca cgtcgggatc
    14221 tcggatcgcg cgctcgccct ccagatcatg aacgaggcgc ggtatttcct gccgcacttc
    14281 ctcgcgctct cgacgaactc tcccttctgg ctgggaagaa atacgggctt gaagagctat
    14341 cgaacgaaag tcttcgagaa gttcccgcgg acgaacatcc cggaggtctt cgaaacgccc
    14401 gccgagttcg acgactacgt ccggatcctc gtcaagacga actgcatcga caacggaaag
    14461 aagatctggt gggacgtccg accgcacccg ttcttcgaca cgatcgagtt ccgcgtctgc
    14521 gatgtcccga tgcgcgtcga cgagacggtc gcgatcgcgg ccctgatcca ggcgatctgc
    14581 gcgaagctct tccagctgcg cgagaagaac ctcggatggc ggacctaccg gcgcgcgttc
    14641 cttctcgaga acaagtggcg cgcgagccgc tacggcgtcg aaggaaagct gatcgacttc
    14701 ggcaaggaga tcgaggtgcc gttccgcgac ctcgccgagg agatgctcga gttcgtcgac
    14761 gacgtggtcg atgagctcgg gagccggcgg gaggtcgagt cgatccgatg gatcctggaa
    14821 aacgggagcg gcgccgaccg gcagcttgcc gtttatcgtg agagcggcga agatctgcgg
    14881 aaagtggtgg actacatctg cgaagagacg cggcacgggc tggacgtcgc ccagtccggc
    14941 agcgcggcgg caatcggatg acgatcgagc gaaacggcaa cggaaacggc tatgacctcg
    15001 ttcccggagc gcgcaacgcc atccggacgt gcctgcgtgt ggcgcccgaa gagcacgtcg
    15061 cgatcctgac cgatcgcgag accgaaccgg tcgcggcgag cctggccgcg gagatccgcg
    15121 aggtcggcgc cccctacgag ctcctcgtcc tcgaggacta cggcgatcgg ccgatgttcg
    15181 agctgccgca gccggtgcgc gaagcgttcg agcgctcggc ggtttcgatc tacgccgggc
    15241 aaccggcccc gggcgagctc gcttatcgcc gggagatgac ggcgatcgtg aaccgtcgga
    15301 agatccggca cgcgcacatg gtttcgatct ccccgcggat catggccgaa gggatgcgtg
    15361 cggacttcga ggaggtcaac gcgatctcga cccgcctgcg cgaccgcgcc gtcaaggccg
    15421 agcggatccg cgcccgttcc cgcgcgggga gcgacctcga ggcgaccttc gacccctccg
    15481 tgaaatggct gaagacgagc gggctgatca cgccggacaa gtgggcgaac ctgccgggcg
    15541 gcgaggtctt caccgcgccg gcgcgcgtgg acggcgtcta cgtcgtcgac ggagttctcg
    15601 gcgattacct ctgcgcgcgc tacggagacc tcgaagagac gccgctcacg gtgtggatcg
    15661 aagacagccg tgtggcccgc gccgagtgcg cgcggtccga cgtccgggac gatttcctcg
    15721 agtacatcga caccgcggag aactccaacc gcgtcggcga gttcgccatg ggcacgaaca
    15781 tcgccgttga ctcgatcatc ggcaatatcc tccaggacga
//
LOCUS       DASWAV010000265         4861 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U2665
            k141_52640, whole genome shotgun sequence.
ACCESSION   DASWAV010000265 DASWAV010000000
VERSION     DASWAV010000265.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960316
            Sequence Read Archive: SRR10260007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 4861)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4861)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:47:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,929
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4861
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_52640"
                     /isolate="SMAG_U2665"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.36444 N 132.31889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..344
                     /locus_tag="VF958_05445"
     CDS             <1..344
                     /locus_tag="VF958_05445"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012527774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HEX9148591.1"
                     /translation="WRAEPHHQAFEGVLNGGIVGALFDCHSNWTAAYHLMKKARADLP
                     PSTVTSEFHVKLARPTPMDTPLTLRAHVVESTENRAVVDATLEANGKVTATCRGTFVA
                     VKEGHPAYHRW"
     gene            complement(426..2189)
                     /locus_tag="VF958_05450"
     CDS             complement(426..2189)
                     /locus_tag="VF958_05450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9148592.1"
                     /translation="MTGIGNRKSGIGNRRAGAVFAILLTLPAGALVADSPDRDEFKRT
                     FEKTVPLRSGQKLQVEHANGKVQIRTHRDPQVSISAAIRVSSSDVEGARRFSEQIQIS
                     VEESPSGVSVVTRYPEKKWNFVGRGFVSYSVDYDIVMPETAPLEVRNKFGDVDVQGLK
                     ASADVKNSNGSIQLRDGKGAQRLENSFGSIDLARNEGGATIVNSNGSVTVADVKGELE
                     LTNRFGSVSATRLSSRSRINNTNGSVQIQDVGGPVEIVSSFGSTTARNVNGDLAARTN
                     NGSIETSNVQGSAELESSFGSIRFSDVKKRVTCTGTNAKVVGVRAGETVSVRTTFGAV
                     EISDAAGVDIENGNGKITVRAIRGGALLKTSFGGIEASDVRGNVTAVNSNAGVMLTDV
                     GGSADVRAGFGKVSLTRIKGGAKVSSENGGVALADIGGPASVKTSFGTLEASRVRGDL
                     TAENSNGAIKASDIQGSVNAKTSFASVSLDGVRESVDVVNQNGAVEVGTPAGADCRRV
                     SVRTSFAPIRVWLPEGGGYTVAARTSFGKVTTEIPVGASGSMGGESLSGKIGDGRCEL
                     TLTNSNGNIDLLKGSGLHLPH"
     gene            complement(2186..2869)
                     /locus_tag="VF958_05455"
     CDS             complement(2186..2869)
                     /locus_tag="VF958_05455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9148593.1"
                     /translation="MTGECRGLDAALEAGDAVAIEAARAHAQECVRCGERLRAFDEIA
                     AAAPHLKKSWESPELWPRIRESLVKESFAASAAPVEHERAPRMAPMWPAWIPIAAIAT
                     LFVVSFVGLQVFRPALGDRQIFGVRSFPKDALMTDAALQDVEKSEREYVRSIENLARV
                     AEPRLSAADTPLLLSYREKLLLLDSAIGELSGQIEQNRYNTHLRRQLLGIYAEKQQTL
                     REVMKGATS"
     gene            complement(2866..3723)
                     /locus_tag="VF958_05460"
     CDS             complement(2866..3723)
                     /locus_tag="VF958_05460"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HEX9148594.1"
                     /translation="MFASHTNPLRARRGEGVHPGASKENGEQDRCDQWVHRGLRPLNT
                     PISPRIPQTVRLPAASKGLKERSRARHPGPSADDLLNIAHEKEVCLGQRDTPTPPGGT
                     EDELLEGCRAGDLLAFETLYERHAPRMKSIARNLLGNAADAEDAVQEAFLKIYRNAAT
                     FRGGAAFSTWIYRVVVNACYDALRRRKTRPAGPSLETEDGRELALPARAPSDHPLRLA
                     LERSVARLAPKHRAVFLLFEVEGFTHGEIARILGIPEGTSKTFLFDAKKKLQRWLAPR
                     PGSRRTVSP"
     gene            3616..4560
                     /locus_tag="VF958_05465"
     CDS             3616..4560
                     /locus_tag="VF958_05465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9148595.1"
                     /translation="MYPLIATILFAILFGGAGMNPLSAPRAERVGVRGEQSSPPSSFE
                     SARFGRYWYQGKAELTRYKLDQARYGEMHSGEAVLIFVTEPFLAERQVKLEHGDPAKG
                     LTVLKLNFVRKFFTGIYPYSIMTSTFTPVDFARTRTLKVASESQEWCGLTFTQLNLRN
                     GRYAGQLRSYFEDEGDRAFDFAPAWLEDELWTRIRLAPETLPVGDVTVVPGLQYARLW
                     HKEVRPENARATLGADGDRNVYTLDYAAIPRKLVIRFEKAFPYKIISWEETQPGAFDG
                     SPLLTTKAVATGSLNLDYWRRHGNADAHYRKELGLTMW"
     gene            complement(4565..>4861)
                     /locus_tag="VF958_05470"
     CDS             complement(4565..>4861)
                     /locus_tag="VF958_05470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011686553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008241 - peptidyl-dipeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M2 family metallopeptidase"
                     /protein_id="HEX9148596.1"
                     /translation="NVPYTRYFLAAILQFQFHRALAHAAGCTGPLHRCSIYASKEAGS
                     KLDTMLQMGMSKPWPDALEAITGQREMDATAIRDYFAPLQKWLDEQNKGKPVGW"
BASE COUNT          789 a         1621 c         1583 g          868 t
ORIGIN      
        1 agtggcgggc ggagccccac caccaggcct tcgagggggt tctgaacggc gggatcgtcg
       61 gcgcgctatt cgactgtcac agcaactgga cggcggccta tcatctgatg aagaaggccc
      121 gtgcggattt gccgccgtcc actgtaacct cggagttcca cgtcaagctg gcgcgcccga
      181 cgccgatgga tacgccgctc acgctgcgcg cgcacgtcgt cgagtcgacg gagaaccgtg
      241 ccgtcgtcga tgcgacgctc gaggcgaacg gaaaggttac cgccacctgc cgcggcactt
      301 tcgtcgcggt caaggaggga catcccgcgt atcaccggtg gtgaggtctt tcgagggttc
      361 cctccagcca tccgcgaccc gcttctctcc agggcggccg gatgtggcaa gtggagacct
      421 gaccctcaat gtggcaagtg gagacctgac cccttcagca gatcgatgtt tccgttgctg
      481 ttcgtcaacg tcagctcgca ccggccgtcg ccgatcttcc cggagagcga ctcaccgccc
      541 atcgatcccg acgcaccgac ggggatttcg gtggtgacct tcccgaagga tgttcgcgcc
      601 gccaccgtgt agcctccgcc ctccggcagc cagacccgga tcggcgcaaa cgacgtccgc
      661 acgctgacgc ggcggcagtc tgcgcccgcg ggggtcccga cctcgaccgc tccgttctgg
      721 ttgacgacgt cgacgctctc gcgcacgccg tcgagactga ccgaggcaaa cgaggtcttc
      781 gcgttgacgg agccctggat gtcggaggcc ttgatcgcgc cgttcgagtt ctccgccgtc
      841 aggtcaccgc gaaccctgga tgcctcgagc gtcccgaagc tcgtcttgac ggaggcgggc
      901 ccgccgatgt ccgcgagcgc gacgccgccg ttttccgacg agactttcgc gccccctttg
      961 atccgcgtca gcgaaacctt tccgaagccg gcgcgaacgt cggccgagcc gccgacgtcc
     1021 gtgagcatca cgcccgcgtt cgagttgacg gcggtcacgt ttccgcggac gtcggacgcc
     1081 tcgatccccc cgaagctcgt cttcagcagc gcccctccac gaatcgcgcg gacggtgatc
     1141 tttccgtttc cgttctcgat atccacgccc gccgcgtcgg agatctcgac ggcaccgaag
     1201 gtggtgcgga ccgacaccgt ttcgcccgcg cgcacgccaa ccacctttgc gttcgttccc
     1261 gtacaggtga cgcgcttctt cacgtccgag aagcggatgc tgccgaacga gctttcgagc
     1321 tccgcgctcc cctggacgtt ggacgtctcg atcgagccgt tgttggtcct ggccgcgaga
     1381 tcgccgttga cgttcctggc cgtcgtcgat ccgaaagatg agacgatctc gacgggcccg
     1441 ccgacatcct ggatctggac gctgccgttg gtgttgttga tccggctgcg cgaggaaagt
     1501 ctcgtcgcgg agacgcttcc gaaacggttc gtcaactcga gttccccctt gacgtcggcg
     1561 accgtgacgg acccgttgct gttgacgatc gtcgcgcctc cttcgttgcg cgcaaggtcg
     1621 atactgccga acgagttctc gagcctctgc gcgccttttc cgtcccggag ttggatcgag
     1681 ccgttcgaat tcttcacgtc ggcggaggct ttcaacccct gaacgtcgac gtcgccgaac
     1741 ttgttgcgga cttcgagagg cgccgtttcg ggcatgacga tgtcgtagtc caccgagtag
     1801 gacacgaagc cgcgcccgac gaagttccac ttcttctcgg ggtaccgggt cacgaccgaa
     1861 acgcccgacg gcgactcttc cacgctgatc tggatctgct cggagaaacg ccgggcccct
     1921 tccacgtccg acgatgacac ccggatcgct gcggagatcg agacctgcgg atctcgatgc
     1981 gtccggatct gcaccttgcc gttggcgtgc tcgacctgga gcttctggcc ggaccggagc
     2041 ggcacggtct tttcgaacgt tcgcttgaac tcgtcccgat ccggcgaatc cgcgacaagc
     2101 gctcctgcgg gcagcgtcag cagaatcgcg aaaacggcgc cggcgcgtcg attcccgatt
     2161 cccgatttcc gatttccgat tccggtcatg acgtggctcc cttcatgacc tcgcgcaggg
     2221 tctgctgctt ttcggcgtag atccccagca gctgccggcg gaggtgcgtg ttgtaacggt
     2281 tctgctcgat ctggccggag agctccccga tcgcgctgtc gagcagaaga agcttttcgc
     2341 gataggaaag gaggagcgga gtgtctgcgg cactcagccg cggctccgcg acgcgtgcga
     2401 ggttctcgat ggaccgcacg tattcgcgct cgctcttttc gacgtcctgg agcgcggcat
     2461 cggtcatcag agcgtccttc ggaaaggagc gcacgccgaa gatctgacgg tcgccgagcg
     2521 ccgggcggaa gacctggagg ccgacgaagg agacgacgaa cagcgtcgcg atcgcggcga
     2581 tcgggatcca ggcgggccac atcggcgcca tccggggggc gcgctcgtgc tcgacgggcg
     2641 ccgccgacgc cgcgaaagac tccttgacga gggactcccg gatcctcggc cagagctccg
     2701 ggctctccca gctcttcttc aggtgaggcg cggccgctgc aatctcgtcg aaggcacgaa
     2761 gccgctcgcc acagcggacg cactcctgcg cgtgggcgcg cgcggcttca atcgcaacgg
     2821 catccccggc ctcgagggcc gcgtcgaggc cgcggcattc cccggtcatg gcgaaaccgt
     2881 ccgccgggag cccgggcggg gagcgagcca gcgttggagc ttcttcttcg cgtcgaagag
     2941 aaacgtcttc gaggttcctt cgggaatccc gaggatgcgc gcgatctcgc cgtgcgtgaa
     3001 cccctcgacc tcgaaaagca ggaacacggc gcgatgcttc ggcgcgagcc gcgcgaccga
     3061 cctttcgagc gcgaggcgaa gcggatggtc ggaaggcgcc cgagccggca gcgcgagctc
     3121 gcggccgtct tccgtctcga gcgacggacc cgcgggccgc gtctttcggc ggcgcagggc
     3181 gtcgtagcag gcgttgacga cgacgcgata gatccacgtc gaaaaggcgg cgccaccccg
     3241 gaaggtcgcg gcgttgcggt agatcttcag aaacgcctcc tggaccgcat cttccgcgtc
     3301 cgccgcgttt cccagcaggt tccgggcgat cgatttcatc cgcggcgcgt gcctttcgta
     3361 gagcgtctcg aaggccagga ggtcgcctgc gcggcagccc tcgaggagct cgtcttccgt
     3421 ccctccggga ggcgtcgggg tatcccgctg gcccaggcag acctcctttt cgtgggcgat
     3481 gttaagcaga tcgtctgcgc tgggaccggg atggcgggcc cggctcctct ccttcagtcc
     3541 cttggacgca gcgggaaggc ggacggtttg aggaatccgg ggagagatgg gcgtatttag
     3601 gggtcggagg cctcgatgta cccactgatc gcaacgatct tgttcgccat tctctttgga
     3661 ggcgccggga tgaaccccct ctccgcgccg cgcgcggaga gggttggtgt gagaggcgaa
     3721 cagtcgtcgc ctccgagctc cttcgagtcc gcccgcttcg gccggtactg gtaccaaggg
     3781 aaggcggagc tcacccgcta caagctcgat caggcgaggt acggcgagat gcactccggc
     3841 gaggcggtgc tcatcttcgt caccgagcct ttcctcgccg agaggcaggt caagctcgaa
     3901 cacggcgacc ccgcgaaggg attgacggtc ctcaagctga atttcgtgag gaagttcttc
     3961 acggggatct atccctactc gatcatgacg tcgaccttca cgcccgtgga cttcgcgcgg
     4021 acgcggacct tgaaggtcgc ctccgagagc caggagtggt gcggcctgac gttcacgcag
     4081 ttgaacctcc ggaacggccg ctacgcgggg cagctacgct cctacttcga ggacgaaggg
     4141 gaccgcgcgt tcgacttcgc gcccgcatgg ctcgaagacg aactgtggac gcgcatccgc
     4201 ctggcacccg agacgctgcc ggtcggcgac gtcacggtcg tgcccggact ccagtacgcg
     4261 cggctctggc acaaagaggt ccgaccggag aacgcgcgcg cgacgctcgg cgccgacggc
     4321 gaccggaacg tctacacgct cgactacgcc gcgatcccgc gcaagctcgt catccggttc
     4381 gagaaggcgt ttccatacaa gatcatctcg tgggaggaga cgcagcccgg cgcgttcgac
     4441 ggaagtccgc tgctgacgac gaaggcggtg gcgaccggga gcctcaacct cgactactgg
     4501 cgccgccacg gcaacgccga cgcgcactac cgaaaggaac ttggtctgac gatgtggtga
     4561 ggtttcacca ccccacgggc ttgcccttgt tctgctcgtc cagccatttc tgcagcggcg
     4621 caaagtagtc gcggatcgcg gtcgcgtcca tctcgcgctg gccggtgatc gcttcgagcg
     4681 cgtccggcca gggcttcgac atccccatct gcagcatggt gtcgagcttg ctgcccgcct
     4741 ccttgctcgc atagatggaa caccggtgga gcggcccggt gcagccggcc gcgtgcgcca
     4801 gagcgcgatg gaactggaac tgcaggatcg ccgcgagaaa gtatcgcgtg tagggcacgt
     4861 t
//
LOCUS       DASWAV010000266         2359 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U2665
            k141_94433, whole genome shotgun sequence.
ACCESSION   DASWAV010000266 DASWAV010000000
VERSION     DASWAV010000266.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960316
            Sequence Read Archive: SRR10260007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2359)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2359)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:47:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,929
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2359
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_94433"
                     /isolate="SMAG_U2665"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.36444 N 132.31889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..198
                     /locus_tag="VF958_05475"
     CDS             <1..198
                     /locus_tag="VF958_05475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011985897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9148597.1"
                     /translation="PASGSISPAIPATNTGTVHDEVGRIIVADVSAEGVKALLAPDRA
                     RLDALIERRYRANVADSGGAD"
     gene            complement(205..281)
                     /locus_tag="VF958_05480"
     tRNA            complement(205..281)
                     /locus_tag="VF958_05480"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(245..247),aa:Pro,seq:cgg)
     gene            complement(326..>2359)
                     /locus_tag="VF958_05485"
     CDS             complement(326..>2359)
                     /locus_tag="VF958_05485"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HEX9148598.1"
                     /translation="AKDPVIGRLVAIKTIRASGGDEDDTQEFRERFVREAQTAGILSH
                     PNIVTIHDIGEDAETETSYIAMEYIEGKNLKMLLAEKTRFPYEQIAEMIAEIAEALDY
                     AHRKGIIHRDVKPANVIITTDGKVKITDFGIAKIASSNLTTTGQFLGTPNYMSPEQVS
                     GSPVDGRSDLFSLGVVLYELLTSKKPFQGDNLTAISYKIVHEDFTPPADLAPEVPIEF
                     NDIVARAMAKDPWNRYQRGKDMALALYQLKAQLEERRSQEDLGTMITVAAENVPTLRL
                     ANLKEIAESGAPDPQETPAAMPAVPDPGAGTRPMSHEGSGSFSQSGSRRTTVTAAPAD
                     VEPTVVESSTSPGRPAPTVPVRPISGKERTAGKKSPPMGLIAIAAVVAVLAVGGYFAL
                     RPKAASSTAAGAAADTSPPNADAVAIGKSFEEGKRLYDEGKYEESLVALRTVLSRDPN
                     NAEAKQLADQAENAIKVRTEQTGREAQIASALDLARQSFTSKKWEEARDRADAVLVLD
                     AQNAEATKLKADSVTQIEKVAAEKKRVEAQLAKIRAMPKTPLPSAQNTQPMGPVAPPP
                     APAAVSNATLRLLFDSPISEGHVMVAVNDQILLRRPYNFKKKSGLFSKSVEATGTVSA
                     NIPVPTGPVTVKVWLSGPGITPAFGTATASMLPSETRTLKVDYGGGQLGVRVQ"
BASE COUNT          320 a          783 c          808 g          448 t
ORIGIN      
        1 ccagcgtccg gcagcatcag cccggcgatc ccggctacga acacaggtac cgtccacgac
       61 gaggtcggcc ggatcatcgt cgcggacgtg agcgcagagg gcgtcaaggc cctgctcgca
      121 cccgaccgag cccggttgga cgccttgatc gagcggaggt atcgcgcaaa tgtggcggat
      181 tctggcggcg ccgactgaaa aggttggtcg gggcgagagg atttgaacct ccgaccactc
      241 ggtcccgaac cgagtgcgct accaggctgc gctacgcccc ggggagccgg gagttttacc
      301 tgattcggcc gctcagtgca aagcgctact ggacgcgcac gccgagctgg ccgccgccgt
      361 agtcgacctt cagcgttcgg gtctccgacg gaagcatgct cgcggtcgcc gtgccgaacg
      421 ccggcgtgat ccccggtccg gacagccaca ccttcaccgt caccggaccg gtcggaacgg
      481 ggatgttcgc cgacacggtg cccgttgcct cgacgctctt gctgaacagc cccgacttct
      541 tcttgaagtt gtaggggcgc cgcagaagga tctgatcgtt gaccgccacc atgacgtggc
      601 cttcggagat cggcgagtcg aagagaaggc gcagcgtcgc gttgctcacg gccgccggcg
      661 cgggcggagg cgcgacgggc cccatcggct gcgtgttctg cgcggacgga agcggggtct
      721 tgggcatcgc gcgaatcttc gcgagctgcg cttcgaccct tttcttttcg gcagcgactt
      781 tctcgatctg cgtcaccgag tcggctttca gcttcgtggc ctcggcgttc tgcgcgtcga
      841 ggacgagcac ggcgtccgcc cggtcgcgcg cctcctccca cttcttcgac gtgaacgact
      901 gccgcgcgag atcgagcgcg ctcgcgatct gcgcctcgcg gcccgtctgc tccgtgcgca
      961 ccttgatcgc gttctccgcc tggtcggcga gctgtttggc ctccgcgttg ttcgggtcgc
     1021 gggaaagcac ggttcgcagc gcgacgaggc tctcctcgta cttgccctcg tcgtacagcc
     1081 gcttgccctc ctcgaacgac ttgccgatcg cgaccgcatc ggcgttcggc ggagacgtgt
     1141 cggcggcggc gcccgctgcg gtcgaggacg ccgctttcgg ccgcagcgcg aagtagccgc
     1201 cgaccgcgag aaccgcgacg acggccgcga tcgcgatgag ccccatcgga gggctcttct
     1261 tccccgccgt tcgctccttg cccgagatcg gacggaccgg gacggtcggc gccggtcggc
     1321 ccgggctggt cgagctctcg acgacggtgg gctccacgtc cgcgggtgcc gccgtcacgg
     1381 tcgtgcggcg cgatccgctc tgcgagaacg agcccgaccc ttcgtgagac atcggccgcg
     1441 tccccgcgcc cggatcgggc acggccggca tggccgctgg agtctcctgc ggatcgggag
     1501 caccgctttc ggcgatttcc ttgaggttcg cgagccgcag ggtcgggacg ttttccgccg
     1561 ccacggtgat catggtcccg aggtcttcct gcgacctgcg ctcctcgagc tgcgccttca
     1621 gctggtagag cgcgagcgcc atgtccttgc cgcgctggta gcggttccag gggtccttcg
     1681 ccatggcgcg ggcgacgatg tcgttgaact cgatcggcac ttcgggggcg agatccgcgg
     1741 gcggagtgaa gtcctcgtgg acgatcttgt aggagatcgc cgtcaggttg tctccctgga
     1801 acggcttctt gctggtcagg agctcgtaga gaacgacccc gagcgagaac aggtcgctcc
     1861 ttccatctac cggtgagccc gagacctgct ccggcgacat gtagttcggg gtgcccagga
     1921 attgcccggt ggtggtcagg ttggaggagg cgatcttcgc gatgccgaag tcggtgatct
     1981 tcaccttgcc gtcggtcgtg atgatgacgt tcgccggctt gacgtcgcgg tggatgatcc
     2041 ccttgcggtg ggcgtagtcg agcgcctccg cgatctcggc gatcatttcg gcgatctgct
     2101 cgtacgggaa gcgggtcttc tccgccagga gcatcttcag gttcttcccc tcgatgtact
     2161 ccatggcgat gtaggacgtc tcggtctcgg cgtcctcgcc gatgtcgtgg atggtgacga
     2221 tgttcgggtg cgagaggatg cccgcggtct gcgcctcgcg cacgaaccgc tcgcggaact
     2281 cctgggtgtc gtcctcgtcg ccgccgctcg cccggatggt cttgatcgcg acgagccggc
     2341 cgatgaccgg gtccttggc
//
LOCUS       DASWAV010000267         3720 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U2665
            k141_653717, whole genome shotgun sequence.
ACCESSION   DASWAV010000267 DASWAV010000000
VERSION     DASWAV010000267.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960316
            Sequence Read Archive: SRR10260007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 3720)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3720)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:47:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,929
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3720
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_653717"
                     /isolate="SMAG_U2665"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.36444 N 132.31889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..352
                     /gene="ruvA"
                     /locus_tag="VF958_05490"
     CDS             <1..352
                     /gene="ruvA"
                     /locus_tag="VF958_05490"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00084.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009379 - Holliday junction helicase
                     complex [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HEX9148599.1"
                     /translation="IGPRVALTVLSGIPPADLIDALSRQDARRLMAVPGVGKKTAERM
                     VLELSEKAAKFAIGPAMPRAATVAPEDVVSALVNLGYRKGDAERAIDAIGRSGAPPEF
                     GEYLKLALKKLTGG"
     gene            378..1400
                     /gene="ruvB"
                     /locus_tag="VF958_05495"
     CDS             378..1400
                     /gene="ruvB"
                     /locus_tag="VF958_05495"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013534281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HEX9148600.1"
                     /translation="MVEVKEILSGTALADDVAVEPTLRPKDLSEYIGQKKVCENLAVF
                     LAAARGRGEPLDHVLLTGPPGLGKTTLAYIIARELGAGLRMTSGPMIARAGDLAGILT
                     NLQPRDVLFLDEIHRLSPAVEEILYPAMEDFRLDVLIGEGPMARTMKVDLPPFTLVGA
                     TTRPGLLSQPLHGRFGITLRLDFYDVESLARIVERSAKILGITIAPDAAREIGARSRG
                     TPRIANRLLRRLRDFADVAGKRAIDLSTAREALERLEVDEHGFDELDRRILATIIEKF
                     GGGPVGINSIAASLGEDRGTLEDLYEPYLIQAGFLQRTPRGRIASANAYRHLGILPPR
                     KEGSLF"
     gene            1415..2284
                     /locus_tag="VF958_05500"
     CDS             1415..2284
                     /locus_tag="VF958_05500"
                     /inference="COORDINATES: protein motif:HMM:NF039905.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HEX9148601.1"
                     /translation="MYNPTAGQRDRAAKMQEFITRMRADGLELINSPTTAPGHATEIV
                     RSLLPAGLDVVAVCGGDGTVSEAACGLVGSTVPLAILPAGTSNVLARELSIPLQLDPA
                     AKLLLEGVPRLVRVLFANERAFLLWAGVGLDARIMGHMSLVLKRWLGRTGIFFTVADE
                     FFRYEFPRLEVTVDGERHEATFAVVCHAQHYAGPWIIAPDARLDSDELDVMLFSDRSR
                     WQFLSLFRQMQLARGGHLRGRLARIVRGRTATVRSLETYPIEVQVDGDCVLETPINCR
                     VAEETISILVPKD"
     gene            complement(2286..>3720)
                     /locus_tag="VF958_05505"
     CDS             complement(2286..>3720)
                     /locus_tag="VF958_05505"
                     /inference="COORDINATES: protein motif:HMM:NF012766.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aminopeptidase P N-terminal domain-containing
                     protein"
                     /protein_id="HEX9148602.1"
                     /translation="SRVRVQIGLIGLLALLVPTPGRSVDFPPVKPPATMFRAHREKFL
                     AKLPPNSIAILHATPLKTMSNDVEYLYRQDSDFWYLTGIEDPDASAVFRPNAADGRRY
                     VVFVQPHDARVESYDGPRMGPAEAAVAYGADQAFSAEEFLDRMMKYDAASRTYGGYLG
                     GVEKLYLADGGDHEWAERVRDDVARMRARDAGPSTVVDARELIHEMRIVKDAEEIALI
                     RRATEISARAHALAMKAAAPGRFEFEVQQALDSYCFANGVRRMAYPSIVASGPNSVFL
                     HWDRNDRRVEDGDVILNDSGAEYGYYATDITRTYPASGRFSAEQRAIYEIVLAAQKAA
                     MAEVKPGAPHARVGLAAARVQTEGLVRLGLLKGDVEQLVKDNGHRALTKHGISHWVGL
                     DVHDAGAYSTGLGSRILEPGMVFTIEPGIYVPSNAPGVDRKWWNIGVRIEDVVLVTPG
                     GFECLSCSAPREIDDVEKTVQSGRAKP"
BASE COUNT          578 a         1279 c         1253 g          610 t
ORIGIN      
        1 catcggaccg cgcgtcgccc tgacggtgct ctccggcatt ccgcccgcgg acctgatcga
       61 cgcgctctcc cgacaggacg cgcggaggct gatggccgtg cccggggtcg gcaagaaaac
      121 ggcggaacgg atggtgctcg agctctcgga gaaggcggcg aagttcgcca tcgggcccgc
      181 gatgccgcgc gcggcgacgg tggcgccgga agacgtcgtt tcggcgctcg tcaacctcgg
      241 gtaccgcaag ggagacgcgg aacgcgccat cgacgcaatc ggccgcagcg gggcgccgcc
      301 ggaattcggc gagtatctga aactcgccct gaagaagctg acgggaggat gagaggagtc
      361 ccttagactc tttcgggatg gtcgaggtga aggagatcct ttccgggacc gcgctcgcgg
      421 acgacgtcgc cgtcgagccg actctgcggc cgaaagacct ctcggaatac atcggccaga
      481 agaaggtctg cgaaaacctg gccgtgtttc tcgccgccgc gcggggccgc ggcgagccgc
      541 tcgatcacgt tctcctgacc ggaccgcccg gcctcggaaa gaccacgctc gcgtacatca
      601 tcgcgcgcga gctcggcgcc ggcctgcgga tgacctcggg tccgatgatc gcccgcgccg
      661 gcgatctcgc cggcatcctg accaacctcc agccgcgaga cgtcctcttc ctggacgaga
      721 tccaccgtct ctcccccgcg gtcgaggaga tcctctatcc ggcgatggag gacttccggc
      781 tggacgtgct catcggcgaa ggtccgatgg cgcgcacgat gaaggtggat ctccccccct
      841 tcacgctcgt cggggcgacg acgcggccgg ggcttctctc tcagccgctg cacgggcgct
      901 tcggcatcac gctccggctc gacttctacg acgtcgagtc gctcgcccgc atcgtggagc
      961 gctcggcgaa gatcctcggg atcacgatcg cgcccgacgc cgcgcgcgag atcggagcgc
     1021 ggagtcgcgg gacgccgcga atcgccaacc ggctgttgcg gcgcctgcgc gacttcgccg
     1081 acgtggccgg caagcgggcg atcgacctct cgaccgcgcg ggaggcgctc gagcggctcg
     1141 aggtggacga gcacggtttc gacgagctcg accgccgcat cctcgcgacg atcatcgaga
     1201 aattcggtgg cgggccagtc gggatcaact cgatcgcggc atcgctcggc gaggacaggg
     1261 gcacgctcga ggacctctac gagccctacc tcattcaggc gggcttcctg cagagaacgc
     1321 cgcgagggcg cattgcgtcg gcaaacgcgt atcgacacct cggaatcctg ccgcctcgaa
     1381 aagagggctc tctgttctga agcacggcgt tctgatctac aacccgacgg ccggacagcg
     1441 cgaccgcgcc gcgaagatgc aggagttcat cacccgtatg cgcgccgacg ggctggagct
     1501 catcaactcc cccacgaccg cgcccggcca cgccacggag atcgtgagaa gtcttctccc
     1561 ggccggcctg gacgtcgtcg cggtgtgcgg cggggacgga acggtttcgg aggcggcgtg
     1621 cggtctcgtc gggtcgacgg ttccgctcgc gattctcccg gccggcacct ccaacgtgct
     1681 ggcgcgtgag ctgtcgatcc cgctccagct cgacccggcc gcgaagctgc tcctcgaagg
     1741 ggttccccgc ctcgtgcggg tcctcttcgc gaacgaacgg gcgttcctgc tgtgggcggg
     1801 cgtggggctc gacgcgcgga tcatggggca catgagcctc gtcctgaagc gttggctcgg
     1861 acggaccggc atcttcttca ccgtcgccga cgagttcttc cgctacgagt tcccccggct
     1921 ggaagtcacg gtcgacggcg aacgccacga ggcgacgttc gcggtcgtct gtcacgcgca
     1981 gcactacgcc ggaccctgga tcatcgcgcc ggacgcccgg ctcgattccg acgaactcga
     2041 cgtgatgctc ttctcggacc ggagtcggtg gcagttcctc tcgctcttcc ggcagatgca
     2101 gctcgcgcgc ggcggacacc tgcggggcag gctcgcgcgg atcgtgcgcg gccgcacggc
     2161 gaccgttcgc tcgctcgaga cctatccgat cgaggtgcag gtggacggcg attgcgtgct
     2221 cgaaaccccg atcaactgcc gcgtggcaga agagacgatc tcgatcctgg tgccgaagga
     2281 ctgagtcagg gcttcgctcg tcccgactgg accgtctttt ccacgtcgtc gatctcgcgg
     2341 ggtgcggagc acgagaggca ttcgaatccg cccggcgtca ccagcacgac gtcctcgatg
     2401 cgcacgccga tgttccacca cttccggtcg accccgggag cgttcgacgg gacatagatc
     2461 cccggctcga tcgtgaacac catccccggc tccagaatgc gcgacccgag gccggtcgag
     2521 taagcgcccg cgtcgtggac gtccagaccc acccagtggg agatcccgtg cttcgtgagc
     2581 gcgcggtggc cgttgtcctt gacgagctgc tcgacgtcgc ccttcaggag accgagccgg
     2641 acgaggccct ccgtctggac tcgcgctgcc gccagtccga cgcgcgcgtg gggcgcgccc
     2701 ggcttcactt ccgccatcgc cgccttctgg gcggcgagca cgatttcgta gatcgcccgc
     2761 tgctctgcgc tgaagcggcc gctcgccggg tacgtccggg tgatgtcggt cgcgtagtag
     2821 ccgtactccg cgcccgagtc gttcaggatc acgtcgccgt cctcgaccct ccggtcgttc
     2881 ctgtcccagt ggaggaacac cgagttcgga cccgacgcga cgatcgacgg atacgccatg
     2941 cgccgaacgc cgtttgcgaa acagtacgag tcgagcgcct gctggacttc gaactcgaac
     3001 cgcccgggcg cggccgcctt catcgcgagc gcgtgtgccc gcgccgagat ctccgtcgcg
     3061 cggcggatga gcgcgatctc ctcggcgtcc ttgacgattc gcatctcgtg gatgagttca
     3121 cgcgcgtcga ccacggtgga aggacccgca tcccgcgcgc gcatccttgc gacgtcgtcg
     3181 cggacgcgtt ccgcccactc gtggtcgccg ccatcggcca ggtagagttt ctcgactccg
     3241 cccaggtagc cgccgtacgt gcgcgaggcg gcgtcgtact tcatcatgcg gtcgaggaac
     3301 tcctccgcgg agaatgcctg gtcggctccg taggcgaccg cggcttcggc gggacccatc
     3361 cgcggtccgt cgtaagactc gacgcgcgcg tcgtgtggct gcacgaagac cacgtaccgc
     3421 cggccgtcgg cggcattcgg ccggaacacg gcggacgcgt cgggatcttc gatgcccgtc
     3481 aggtaccaga agtccgagtc ctgccggtac aggtactcga cgtcgttcga catcgtcttc
     3541 agcggcgttg cgtgaaggat cgcgatggag ttcggcggca gtttcgcgag gaacttttcc
     3601 cggtgcgccc ggaacatcgt ggccggaggc ttgacggggg gaaagtcgac cgaccgaccc
     3661 ggagtcggga ccaggagagc caggaggcca atgaggccga tttggactct gactctcgag
//
LOCUS       DASWAV010000268         7222 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U2665
            k141_534459, whole genome shotgun sequence.
ACCESSION   DASWAV010000268 DASWAV010000000
VERSION     DASWAV010000268.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960316
            Sequence Read Archive: SRR10260007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 7222)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7222)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:47:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,929
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7222
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_534459"
                     /isolate="SMAG_U2665"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.36444 N 132.31889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..699
                     /locus_tag="VF958_05510"
     CDS             <1..699
                     /locus_tag="VF958_05510"
                     /inference="COORDINATES: protein motif:HMM:NF013116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase family protein"
                     /protein_id="HEX9148603.1"
                     /translation="AGVPAIQRCLADGVNINITLLFSVNRYREVMEAYLSALAERTAR
                     GRAVENLPSVASFFVSRTDTNVDKKLDRIAKEDGDGPRGRMARDLRGKAAIANARLAY
                     AAFEEVFHSERFRALEKKGARLQRPLWASTSTKDPAYPDLYYVEALVAPNTVDTMPPD
                     TFEAYRDHGDPKVRIHDGLDAARDVFRRLSELGIDEATVSRELEEEGVKKFSDSYESL
                     LKALAEKEKAMRVA"
     gene            870..2342
                     /locus_tag="VF958_05515"
     CDS             870..2342
                     /locus_tag="VF958_05515"
                     /inference="COORDINATES: protein motif:HMM:NF012562.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HEX9148604.1"
                     /translation="MAAVRPQLEGMAERFRAGTDRVLVLGMGGSSLAPLVFASSFGQR
                     PGYPRLEVLDSTHPESVLAAAESAPPERSVYVVSSKSGTTLEPNLFFDWFFARAEKDL
                     GAQAGKRFVVITDPGTQLEKLARSRGVEATVLSDPAIGGRYSALSPFGIVPAVLAGID
                     VAELLRRARATAAACRTGDPEQNPGASLGAAIGALALEGRDKLTFSVSSPVDRFGMWL
                     EQLIAESTGKEGRGVLPVEGEPLGAPDAYGDDRFFVRIGVGPEGSDPAGKLARLEASG
                     HPVARIEISDVLDLGAEMFRWEFATAIAGSLLGIDPFDQPNVQEAKDRTNEILAGKDT
                     GEAAPGAGTKPRALSDLLESIRPGDYFAITAYLPSTADNEERLSRIRLPVRDGKRVAT
                     TVGFGPRFLHSTGQLHKGGPDTGVFLQITAAPSVVLPIPGRPFGFERVIAAQAAGDLA
                     ALRSRGRRALSVDVEDPATGLDSLVSDVTNSVGAMKKEPQ"
     gene            2339..3235
                     /gene="gnd"
                     /locus_tag="VF958_05520"
     CDS             2339..3235
                     /gene="gnd"
                     /locus_tag="VF958_05520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013985980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004616 - phosphogluconate dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="decarboxylating 6-phosphogluconate
                     dehydrogenase"
                     /protein_id="HEX9148605.1"
                     /translation="MKLGFVGLGRMGGNMVRRLLGDGHEIVAWARSEESVKEAVARGA
                     VGATSLEDVASRLTPARVVWLMIPAGDPVEKSVATLRGVLSEGDVIVDGGNSRFSDSA
                     RRAAELEKDGIGFLDAGTSGGIWGFENGYCLMVGGRSDHFRKVEPAFKTLAPPDGYAH
                     VGAPGSGHFVKMVHNAVEYAMLQGYGEGFEMLQASGYDLDLLQISKLWTHASVVRSWL
                     LDLLVRALEADPKLASIKGYVEDSGEGRWTLHEAIERAVPTPALASALFARFSSRQPE
                     SFSAKVIAALRAQFGGHAVAKE"
     gene            3308..4801
                     /gene="zwf"
                     /locus_tag="VF958_05525"
     CDS             3308..4801
                     /gene="zwf"
                     /locus_tag="VF958_05525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008477322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004345 - glucose-6-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HEX9148606.1"
                     /translation="MKTEEELRPPVTLIIFGASGDLTRRLLAPAIAHLSRDGAISPDF
                     AIVGLARTPYSDSEFRTRLEEGTREFTPPEQGRPGELPGPIRYLTGDFGDRELYKKLH
                     QVLDEIESKRSNSRNRIFYLATPPEADPEIVGLLGEQKLSEPENGWSRVVVEKPFGHD
                     LESAKKLNACLRSVFDERQIYRIDHYLAKETVQNVFVLRFANGIFEPLWNQRYVDHVQ
                     ISVAETLGVGHRAGYYEEAGILRDMFQNHLLQLLCLTAMEPPVKFEADAVRAEKVKVL
                     QSLRPFPEDGADAWAVRGQYGSGVVEGKTVPGYREEANVAPDSKTPTYGAVKFVIDNW
                     RWAGVPFYVRSGKRMATRISEIAIEFKSVPHHYFRHQDGNLPPNVLRLRIQPEEAVSL
                     KFEAKIPGAFGQVESVYMDFPYSNLGAGSHGGYERLLVDVTHDDQTLFIRGDEAEEAW
                     RVLDPLLKAWESQPPPEFPNYAAGTMGPEAADHFIQQDGRRWRTLVK"
     gene            4798..5556
                     /gene="pgl"
                     /locus_tag="VF958_05530"
     CDS             4798..5556
                     /gene="pgl"
                     /locus_tag="VF958_05530"
                     /EC_number="3.1.1.31"
                     /inference="COORDINATES: protein motif:HMM:TIGR01198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017057 - 6-phosphogluconolactonase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconolactonase"
                     /protein_id="HEX9148607.1"
                     /translation="MNRLPEEGPPNFEVRVLPDADEAARAAALEIFHAAADAIGNRGV
                     VRIALSGGTTPVRLFRLLAGLPFRDRPEWSRTRFFFVDERCVPPEDARSNYRLAKEHL
                     LDPLGVPAEHVFRMRGEDDPRRAAAEYQSVLEKEFGGQDSVPRFDFVLLGLGTDGHTA
                     SLFPGTAALEERERLVSANWVPKLNEWRLTLTLPVLNASRWALFLVTGAEKSSVVASA
                     VERKDGSRELPASLVRPRAGSLIWIVDQAAAAGL"
     gene            complement(5761..>7222)
                     /locus_tag="VF958_05535"
     CDS             complement(5761..>7222)
                     /locus_tag="VF958_05535"
                     /inference="COORDINATES: protein motif:HMM:NF009381.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HEX9148608.1"
                     /translation="EKLVEMVAEGDDTLMEKFFAQGTLQETELLPGLQKEIAARKIFP
                     VFCASSALQIGVRRVLDACVQLLPSPEETKVTGTGKDGKPVEVACDEKGHAVAQVFKT
                     VSDPFAGRISYMRVYSGHFQSDGTYWNATKATPERFSGLFLPQGKEHLSVPEVRAGDI
                     VAVAKLKETETGDSLSTKDHPVILPKIVVPEPAIAYAIEPKAKGDEDKISTALHKLIE
                     EDPSLRFQRNEETKEFHLAGASQLHVEIAVARLKKRYGVEVILHPPKVPYRETITRRA
                     EAHGRHKKQTGGHGQFADCKIKVEPLPRGSDFEFVDEIYGGSIPRNYIPAVEKGIQDV
                     RKKGFLAGYPMVDFRVILIDGQYHDVDSSELAFKIAGALAYKDAMEKAKPTILEPVMT
                     VEVNAPNEYVGDLMGDLSSRRGKVEGMESGDEDTVIRAQVPMSEMLTYGAQLRSITQG
                     RGSFHLEFDHYSEVPHSIQEKIIAQSKADKAAAEGA"
BASE COUNT         1135 a         2429 c         2452 g         1206 t
ORIGIN      
        1 gcgggagtgc cggcgatcca gcgatgcctc gccgacgggg tcaacatcaa tatcacgctt
       61 ctcttctcgg tgaaccgcta ccgggaggtc atggaggcgt atctctccgc cctcgcggag
      121 cgcaccgcga gggggagagc ggtggagaac ctgccgtccg tcgcgagctt cttcgtcagc
      181 cgcaccgaca cgaacgtgga caagaagctc gaccggatcg cgaaggagga cggcgacggt
      241 ccgcgcggga ggatggcacg cgaccttcgc gggaaggcgg cgatcgcgaa cgcccgcctg
      301 gcctatgccg ccttcgaaga ggtgttccat tccgagcgct tccgggcgct cgagaagaag
      361 ggcgcccgtc tccagcgtcc tctctgggcg tcgacgtcga cgaaggatcc cgcgtatccc
      421 gatctctact acgtcgaggc cctcgtggcg ccgaacaccg tcgacacgat gccgcccgac
      481 accttcgagg cataccgtga ccacggggac ccgaaggttc ggatccacga cggcctggac
      541 gcggcgcgcg acgtcttccg ccgcctgtcg gagctcggga tcgacgaagc gacggtttcg
      601 cgcgagctcg aggaggaggg agtgaagaag ttttccgact cctacgagtc gttgttgaag
      661 gcgctcgcgg aaaaagaaaa ggcgatgagg gtcgcctgaa gctcgctctc ggacgattcg
      721 agaaatcctg gcgggaggct ctccggcggc tcgaacggga gcgggccgtc gagcgcgtct
      781 ggtcgggcga cgccctcctg tggaagaacg atcctgctca ccagcgcacg atcggaaacg
      841 cgctcggctg gctcacgatc gccgaccgga tggcggcggt gaggccgcaa ctcgagggga
      901 tggccgaacg ttttcgagcc gggacggacc gcgtcctggt gctcgggatg ggaggctcgt
      961 cgctcgcccc gctcgtcttc gcgtcctctt tcggtcagcg gcccggatac ccgcggctcg
     1021 aggtcctcga ctcgacccac cccgagagcg tcctcgccgc ggcggagagc gctccgccgg
     1081 aacggtcggt ctacgtcgtc tcgtcgaagt ccgggacgac tctcgagccg aacctcttct
     1141 tcgactggtt cttcgcgcgg gccgaaaaag acctcggggc tcaagccgga aagcgcttcg
     1201 tggtcatcac ggatccgggg acgcagctcg aaaagctcgc caggagccgg ggtgtcgagg
     1261 ccacggtgct ttcggacccg gcgatcggcg gccgatactc cgcactctcc ccgttcggga
     1321 tcgtgccggc cgtgctcgcc ggaatcgacg tcgcggagct gctgcgccgg gcgcgcgcga
     1381 ccgcggccgc ctgtcgcacc ggcgatcccg aacagaaccc gggcgcctcc ctgggcgcgg
     1441 cgatcggcgc gctggcgctc gaaggccgcg acaagctcac cttttccgtc tcgtctcccg
     1501 tcgaccgttt cggcatgtgg ctcgagcagc tgatcgccga gtcgacgggc aaggagggga
     1561 gaggggtcct tccggtggaa ggcgagccgc tcggcgctcc cgacgcgtac ggcgacgacc
     1621 ggtttttcgt ccggatcggc gtcgggccgg aggggtccga tccggcagga aagctcgccc
     1681 ggctcgaggc aagcggacac ccggtcgcgc ggatcgagat ctccgacgtt ctcgacctcg
     1741 gcgcggagat gttccgctgg gagttcgcga ccgcgatcgc cggctctctt ctcgggatcg
     1801 atccattcga tcagccgaac gtccaggagg cgaaggaccg cacgaacgag atcctcgccg
     1861 gcaaggacac cggggaagcg gcgcccggcg ccgggacgaa accccgggcg ctctccgacc
     1921 ttctcgaatc catccggccc ggcgactact tcgcgatcac ggcctacctg ccttcgaccg
     1981 ccgacaacga ggagcggctc tcgcggatcc gccttccggt ccgcgacggt aagcgcgtgg
     2041 cgacgacggt cgggttcggt ccgcggttcc tgcactcgac cgggcagctc cacaagggcg
     2101 gtccggacac cggagtcttc ctgcagatca cggcggcgcc gtccgtcgtg cttccgatcc
     2161 ccggccggcc gttcggcttc gaacgggtga tcgcggcgca ggccgccggc gacctcgcgg
     2221 cgcttcgatc ccgcggacgc cgggcgctct cggtcgacgt cgaggacccg gcgaccggcc
     2281 tcgattcgct cgtctccgac gtcaccaact cggtcggagc gatgaagaag gagccgcaat
     2341 gaagctcggt ttcgtgggac tcggccgcat gggcggaaac atggttcgcc gtcttctcgg
     2401 ggatggccac gagatcgtgg cgtgggcccg aagcgaggag tcggtgaagg aggccgtcgc
     2461 ccggggagcc gtcggggcca cgtccctcga agacgtcgct tcccgcctca ccccggcgcg
     2521 cgtcgtctgg ctgatgatcc ccgcgggcga cccggtcgag aaatcggtcg cgacgctccg
     2581 gggagtcctg tcggaaggcg acgtgatcgt cgacggcggc aactcgcgtt tctccgactc
     2641 cgcgcgccgc gctgcggagc tcgaaaagga tggcatcggg ttcctcgacg ccggcacctc
     2701 cggcggcatc tggggcttcg agaacggcta ctgcctgatg gtcggcggcc gttccgacca
     2761 cttccggaag gtcgagccgg ccttcaagac gctcgcgccc cccgacgggt acgcgcacgt
     2821 cggcgcgccc gggtccggtc acttcgtgaa gatggtgcac aacgccgtcg agtacgcgat
     2881 gctccagggg tacggcgaag gcttcgagat gctgcaggcg tccggctacg acctcgacct
     2941 gctgcagatc tcgaagctct ggacccacgc ctcggtcgtg aggtcctggc tgctcgacct
     3001 cctcgtgcgg gcgctcgagg ccgacccgaa actcgcctcc atcaaggggt acgtggagga
     3061 ctccggcgaa ggccggtgga cgctccacga ggcgatcgag cgcgccgttc cgacgccggc
     3121 gctcgcctcg gcgctgttcg cgcgcttctc ttcgcggcag ccggaatcgt tttccgcgaa
     3181 ggtgatcgcc gcgctgcgcg cgcagttcgg cggccacgcg gtggcaaagg aatgaagacc
     3241 ttgaccgacg aaggttgcac cctcgcccct ctggagggga gagggggccg ggggtgaggg
     3301 gtagccattg aagaccgaag aagaactgcg gcccccggtc acccttatca tcttcggcgc
     3361 gtcgggggat ctcacccggc ggctgctcgc gcccgcgatc gcgcatctct cccgcgacgg
     3421 cgcgatctcg cccgacttcg cgatcgtggg cctcgcgcgg actccttaca gcgactccga
     3481 attccggacg cgcctcgagg agggcacacg cgagttcact ccgcccgagc aggggcgccc
     3541 gggcgaactg cccgggccca tccgatatct gaccggcgac ttcggagatc gcgagctcta
     3601 caagaagctc caccaggttc tcgacgagat cgagtcgaag cgcagcaact cccgcaaccg
     3661 gatcttctac ctcgcgacgc cccccgaagc cgacccggaa atcgtggggc tgctcggcga
     3721 gcagaagctg tccgagcccg agaacggctg gtcgcgggtc gtcgtcgaga agccgttcgg
     3781 tcacgacctc gagtccgcga agaagttgaa cgcgtgtctc cggagcgtgt tcgacgagcg
     3841 tcagatctac cgcatcgacc actacctcgc gaaggagacg gtccagaacg tcttcgtgct
     3901 tcggttcgcc aacgggatct tcgagccgct gtggaaccag cggtacgtcg accacgttca
     3961 gatctcggtc gccgagacgc tcggcgtcgg ccatcgggcc ggctactacg aggaggccgg
     4021 catcctgcgc gacatgttcc agaaccacct ccttcagctc ctttgcctga ccgcgatgga
     4081 gccgcccgtg aagttcgagg cggatgcggt gcgcgccgag aaggtgaaag tgctccagtc
     4141 gctccggccg tttccggaag acggcgcgga cgcctgggcc gtgcgcggtc agtacgggtc
     4201 cggggtcgtc gagggcaaga cggttccggg ctatcgcgag gaggcgaacg tcgccccgga
     4261 ctcgaagacg ccgacgtacg gcgcggtcaa gttcgtcatc gacaactggc gctgggcggg
     4321 cgttcctttc tacgtgcggt cggggaaacg gatggcgacc cggatctccg agatcgcgat
     4381 cgagttcaag agcgtcccgc atcactactt ccgccaccag gacgggaacc tgccgccgaa
     4441 cgtgctgagg ctccggatcc agccggaaga ggccgtgtcg ctcaagttcg aggcgaagat
     4501 cccgggtgca ttcgggcagg tcgagtcggt ctacatggat tttccctact cgaatctcgg
     4561 agcaggatcg cacggcggtt acgaacggct gctggtcgac gtgacccacg acgatcagac
     4621 gcttttcatc cgtggagacg aggcagagga agcctggcgg gtcctcgacc cgctgttgaa
     4681 ggcgtgggaa tcccagccgc cgccggagtt tccgaactat gcggccggga cgatgggccc
     4741 cgaggcggcg gaccatttca tccagcagga cggccggcgc tggcgcaccc tcgtgaagtg
     4801 aaccggctcc ccgaggaggg accgccgaac ttcgaggttc gcgtcctgcc cgacgcggac
     4861 gaggcggcgc gcgctgctgc gctcgagatc tttcacgccg ccgcagacgc gatcggcaac
     4921 cgcggcgtcg tccgcattgc gctctccggc gggacgacac ccgtccgtct cttccggctc
     4981 ctggcggggc ttcctttccg cgaccggccg gagtggagtc gcacccggtt cttcttcgtc
     5041 gacgagcggt gcgtgcctcc cgaggacgcc cggtcgaact atcgtctcgc gaaggagcac
     5101 ctgctcgatc cgctcggagt gcccgcggag cacgtcttcc ggatgcgcgg cgaggacgac
     5161 ccgcgccgcg cggccgcgga ataccagagc gtcctcgaga aagagttcgg cgggcaagat
     5221 tccgtcccgc gcttcgactt cgtcctgctc ggcctcggaa ccgacggcca caccgcgtcg
     5281 ctctttcccg gaaccgccgc tctggaagag cgcgagcggc tcgtctccgc gaactgggtg
     5341 cccaaactga acgagtggcg gctgacgctc acgctcccgg tcctgaacgc ctcccgctgg
     5401 gccttgtttc tcgtcacggg agccgaaaaa agctcggtcg tcgcttcggc cgtcgaacgg
     5461 aaagacggat cccgcgagct gcccgcgtcg ctcgttcgcc cccgcgctgg ctcgttgatc
     5521 tggatcgtgg accaagcagc tgcagcgggg ctatagcagc cgcgagaggt cacatcggat
     5581 cgcggcgcaa ggggtctcct aatgtcgcat cgcgaagagt ctccgcgaag taaccgagcg
     5641 gaggagcttc gcgatgcggg agcgcgagtg ccgcgaggga tcaggcgctc ggtcgaggct
     5701 ggcttgccga gccgagcgcc gttggcgtca gccagatccc gagcaatcga gtgcgacggt
     5761 tcaggctccc tccgccgccg ccttgtccgc cttcgactgc gcgatgatct tttcctggat
     5821 cgagtgcggc acctcggagt agtggtcgaa ctcgaggtgg aacgagccgc gcccctgcgt
     5881 gatcgaacgc agctgcgcgc cgtaggtgag catctccgac atcggcacct gcgctcggat
     5941 gaccgtgtcc tcgtcgccgc tctccatccc ctcgaccttc ccccgccgtg acgagaggtc
     6001 gcccatcagg tcgccgacgt actcgttcgg cgcgttgacc tcgacggtca tcacgggttc
     6061 gaggatcgtc ggcttcgcct tctccatcgc gtccttgtac gcgagcgcgc ccgcgatctt
     6121 gaacgcgagc tcggaggagt cgacgtcgtg gtactggccg tcgatcagga tcacgcggaa
     6181 gtcgaccatc ggatacccgg cgaggaatcc cttcttgcgg acgtcctgga tgcccttctc
     6241 gacggcgggg atgtagttgc gggggatcga gccgccgtag atttcgtcga cgaactcgaa
     6301 atcggagccg cgcggcagcg gctcgacctt gatcttgcag tcggcgaact gcccgtgccc
     6361 gcccgtctgc ttcttgtggc ggccgtgggc ctcggcgcgg cgcgtgatcg tctcgcggta
     6421 ggggaccttg ggcggatgga ggatgacctc gacgccgtag cgctttttta gcctcgcgac
     6481 ggcaatctcg acgtggagct gcgaggcgcc ggcgaggtgg aactccttcg tctcctcgtt
     6541 gcgctggaag cgcagcgacg ggtcctcctc gatcaacttg tggagtgccg tcgaaatctt
     6601 gtcctcgtcc cccttcgcct tcggttcgat cgcgtaggcg atggcgggct ccggcacgac
     6661 gatcttcggc agaatgaccg gatggtcctt cgtcgacagc gagtccccgg tctccgtctc
     6721 tttcagcttc gcgaccgcga cgatgtcgcc cgcgcgcacc tccgggacgc tcaagtgctc
     6781 cttcccctgg ggaaggaaga gcccggagaa gcgctcgggc gtcgccttcg tcgcgttcca
     6841 gtaagtcccg tcggactgga agtgtccgga gtagacgcgc atgtacgaga tccggccggc
     6901 gaaggggtcg gacaccgtct tgaagacctg ggcgacggcg tgtcccttct cgtcgcaagc
     6961 gacctcgacg ggctttccgt ccttgcccgt gcccgtcacc ttcgtttcct cgggcgaggg
     7021 caggagctgg acacaggcgt ccaggacccg gcgcaccccg atctgcagcg ccgaggaggc
     7081 gcagaagacc gggaagatct tcctcgcggc gatctccttc tggagcccgg gcaagagctc
     7141 ggtctcctgg agggtgccct gcgcgaagaa cttctccatc agggtgtcgt caccctccgc
     7201 gaccatctcg accaatttct cg
//
LOCUS       DASWAV010000269         8316 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U2665
            k141_214869, whole genome shotgun sequence.
ACCESSION   DASWAV010000269 DASWAV010000000
VERSION     DASWAV010000269.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960316
            Sequence Read Archive: SRR10260007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 8316)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8316)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:47:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,929
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8316
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_214869"
                     /isolate="SMAG_U2665"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.36444 N 132.31889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..318
                     /locus_tag="VF958_05540"
     CDS             <1..318
                     /locus_tag="VF958_05540"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX9148609.1"
                     /translation="ARLKPRVVLLDLKLSSEFGATLVSVLRRKSARTRVILLVGRAPE
                     AKILEALSHGAVGCVSKKDLVRFLPEALVAVSSGEAWMSRKLIPKIMDRLAAFTARAA
                     GHP"
     gene            353..1732
                     /locus_tag="VF958_05545"
     CDS             353..1732
                     /locus_tag="VF958_05545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011686652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HEX9148610.1"
                     /translation="MTRELIRGISRLDATTLVVGSMIGSGIFIVSAKSARVLGSPGWL
                     LAAWVIAGLLTMVAALCCSELAAMMPRAGGQYVFFREAYGPLFGFLFGWTMFLVVQTG
                     TIAAVAVAFAKFLGVLWPAVSETKTKFGPFSVSIAQGVGVAVIVLLTASNATGLRTGT
                     TTQNAFTFAKLAALLGLTACGLALARPGAALREGGFWAATSPNSQSLTGLALAIALGT
                     AMVGPLFSQSAWNNVTFAGEEVERPGRTLPQALLAGCLLVTTVYVLANVAYLNVLPLH
                     AVQHAPADRVATAAATSLFGPAAATVMALAIMISTFGCVNGLILSGARVSFAMARDGL
                     FFQSLERLNRASVPANALWAQALWASVLTISGSYSDLLEYVISADILFYVLLVVAVIV
                     LRRKRPDLPRPFRAPGYPVLPLLYAAAGAVLIGVLLAGNPRTTWPGYGLVLTGVPVYF
                     AWRRRPMQT"
     gene            1738..3321
                     /gene="pxpB"
                     /locus_tag="VF958_05550"
     CDS             1738..3321
                     /gene="pxpB"
                     /locus_tag="VF958_05550"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: protein motif:HMM:NF014663.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017168 - 5-oxoprolinase (ATP-hydrolyzing)
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpB"
                     /protein_id="HEX9148611.1"
                     /translation="MNLEVHDVSEDSVLVEFPEAPDAEANRAAIEIGARLTAKRRHGL
                     VDAIPGARTLYLAFDHRVIDREAVVRRVRRAADEPAETSSDSRLLRIPVAYGGDNGPD
                     LATLARDLGIDPEEVARRHENAEYRVAFLGFSPGFAYLIGLPVELATPRRDSPRPRVA
                     AGSVAIGGPYTAVYPTESPGGWRLIGRSPVGLFDPFASPPALLRPGDRVRFERIDEDE
                     LARRIAASMEHPRAAAVSEGEPVFAAIAPGLFSSVQGGPRHGLGAYGVPAGGAMDATA
                     LERGNAIVGNPPQAPALEVTLSGPELRVLDDALACVAGGDFQARCNGKLLEIDASFEL
                     KAGDHLAFGYARSGARAYLCVRGGLVERRPGEPLKRLVRGDIVARGRLAERAQAALPR
                     RPDAAGDGVRVLRVLAGPQFALFPPAATDVFFSASYRVSPESDRRGIRLDGPVVALRR
                     LSDIPPEGTALGAIQVPAGGLPIVLGPDRPVTGGYAKIATVIRRDWPLLAQAPPGTAV
                     RFRAVTLAEAIELIGEPLD"
     gene            3333..4106
                     /locus_tag="VF958_05555"
     CDS             3333..4106
                     /locus_tag="VF958_05555"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007321772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_function: GO:0017168 - 5-oxoprolinase (ATP-hydrolyzing)
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpA"
                     /protein_id="HEX9148612.1"
                     /translation="MSIDLNADVGEGMDDGELLPYVTSANVACGMHAGDPVTMDQTVE
                     LALSRGVRVGAHPGYPDRANFGRVMMEMSADEIESLIVYQVSALAGFARSRGSRLTHV
                     KPHGALYHSGAEFPDVARAIAEGVRRVGTDLVLVGAAGSMLIGAGQEAGLPVAEEAFA
                     DRRYRADGTLVPRGHRGALITDPDEAAEQAVGLARDGAAVADDSSRVSVRADTICLHG
                     DTPGAAEIARRIHDRFRAAGIRIAPLETGVKRRDPGGTA"
     gene            4132..4683
                     /locus_tag="VF958_05560"
     CDS             4132..4683
                     /locus_tag="VF958_05560"
                     /inference="COORDINATES: protein motif:HMM:NF018980.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HEX9148613.1"
                     /translation="MTTESDDFSADLPEPGWMERLAAIHRAIHAVLATRAEIFREELA
                     EKGSLFGKGLVGIALALAFAGLSLLVLTALVAALLAKLFGGPIAGITATLVLYLLITA
                     SAALFGIKKLSRVKPLDFPATRDELRKDLDALRKEPPESDEGAASSDALEAEKASVRP
                     GEAGGGDRAIAEDLEDRFRAGSE"
     gene            4680..4991
                     /locus_tag="VF958_05565"
     CDS             4680..4991
                     /locus_tag="VF958_05565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9148614.1"
                     /translation="MTDEKTTRRRGSRAERIEELRRRGAFERDALTRGIAGLRGAIDE
                     KKALWKTVGWIVGIAAAAGTVGYKLFGKHSLSAKIGRVTNVAMILFGLGKAAGKARKL
                     L"
     gene            5172..5729
                     /locus_tag="VF958_05570"
     CDS             5172..5729
                     /locus_tag="VF958_05570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9148615.1"
                     /translation="MKRAIRFAMVAAAMAAVLVVLTPSAAQAQVRVEGRFRLPHGQIA
                     IGVGDPYYGRGYYGDIYYGAPAYPVGSYVPYGYRVIEDPRLGYGFYSPAFGCSLHHVR
                     HRHWIPVRRSTRRWVVIERPYFGGRDYRRGYDSRRYNDRRYNDRRYDQGYRGDPYYDF
                     NREYWDRSYHPYDDPNYGRRPYDRR"
     gene            complement(5818..6603)
                     /locus_tag="VF958_05575"
     CDS             complement(5818..6603)
                     /locus_tag="VF958_05575"
                     /inference="COORDINATES: protein motif:HMM:NF012863.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDSL-type esterase/lipase family protein"
                     /protein_id="HEX9148616.1"
                     /translation="MRYSRFWLWRVPAALALVAGAVFTAGFWLAIRSSSGSPIGAAPP
                     APAPLQAAKNPGKRLVLILGDSLSHGTGDPTGRGYAADVVAALERRGPVESVNLAVAG
                     AESSDLRKLAESANVRSLAASADLILFSVGGNDLSHSLSAASAPAQALSSVGDSRSRY
                     ADNLRSILRDLREANPSAPIRLLGLYQPFGGEGRESRVGESLVLSWNSVLAETALGYA
                     NVAAVPVFDLFQGRPDRLATDRYHPNRQGYRAIADRVIQTLTF"
     gene            6612..7595
                     /locus_tag="VF958_05580"
     CDS             6612..7595
                     /locus_tag="VF958_05580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018751312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX9148617.1"
                     /translation="MIRSSTEDWGLGTDVGPTPHNDTIVLSAEGLTKSFGRREVVSDV
                     SFELRAGEVFGFLGPNGAGKTTTIRMLVGLARPDRGRVRIAGFDLQHDFAKAMARVGC
                     IVESTDLYPYLTGRENLIHFARMLPDGAEKRIPELARLVFLEARLDDRVATYSLGMRQ
                     RLGLAQALLGEPDLLILDEPANGLDPAGIREIRELVRHLAHDRGMAVFVSSHLLSEVE
                     QTCDRVAIIHRGRTLAMGPVRELLESRGAERLVFRASPSARAEEVLAPFGRAGASRIE
                     GEEAVSADVPRERVPEALAALAAAGVLVYAVERQSSTLEEIFLEVTGGETV"
     gene            7606..>8316
                     /locus_tag="VF958_05585"
     CDS             7606..>8316
                     /locus_tag="VF958_05585"
                     /inference="COORDINATES: protein motif:HMM:NF024091.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX9148618.1"
                     /translation="MKFAPLVENEILKLLKRRRFRVALLILVALNGLIVFAQTQSRDR
                     RPRGDWRIEAQERVARMQNWERSGRLPQTQLKWVRFEIARVQYHLDRNMDPEAISGPL
                     FARAFANASSYLLLPLLAILFAADIVSSEFAQGTIKLLLTRPVGRARVLTSKFAALAV
                     AITLTVLLGGVVAYLFGGLAFGYRGWGAPVLTGFRLSGETFDPTAVRAVPLWEDALLA
                     FGLAWYAALTVGAIAFLTS"
BASE COUNT         1201 a         2862 c         2877 g         1376 t
ORIGIN      
        1 gcgcgtttga agccccgtgt cgtactgctc gacctgaagc tctcctcgga attcggagcg
       61 acgctcgtct ccgtcctgcg gcgcaagagc gcccgcacgc gggtcatcct cctcgtcggg
      121 cgcgcaccgg aggcgaagat cctcgaggcg ctctcccacg gcgccgtcgg ctgcgtcagc
      181 aagaaggacc tcgtgcgttt cctcccggag gcgctcgtcg cggtgagctc gggagaggcg
      241 tggatgtccc ggaagttgat ccccaagatc atggaccggc tcgcggcgtt caccgcccgc
      301 gcggcggggc atccgtagaa cgcgcgcggc gcaatcgttc gacgaccgcg gcttgacccg
      361 cgagctcatc cgaggaatca gtcgcctcga cgcgacgacg ctcgtcgtcg gctcgatgat
      421 cggctcgggc attttcatcg tttcggccaa gagcgcccgg gttctcggct cccccggatg
      481 gctgctcgcc gcctgggtga tcgccggtct tctgacgatg gtcgctgcgc tctgctgctc
      541 ggagctcgct gcgatgatgc cgagggcggg cggccagtac gtctttttcc gcgaggccta
      601 cggtccgctc ttcggtttcc tgtttggctg gacgatgttc ctcgtcgttc agaccgggac
      661 gatcgccgcc gtcgccgtcg ccttcgcgaa gttcctcggc gttctgtggc cagccgtctc
      721 ggagaccaag acgaagttcg ggccgttttc cgtctcgatc gcgcagggag tcggcgtggc
      781 cgtcatcgtc ctcctcaccg cctcgaacgc aaccgggctc cgcacgggaa cgaccacgca
      841 gaacgcattc acgttcgcga agctcgcggc gcttctcggg ctgaccgcat gcgggctcgc
      901 gctcgcccga ccgggggcgg cgctgcgtga agggggattc tgggcagcga cctcgccgaa
      961 ctcgcagagc ctcaccgggc tcgccctcgc catcgcgctc gggaccgcca tggtcggacc
     1021 cctcttctcc cagtcggcct ggaacaacgt gaccttcgcg ggcgaggagg tggaacgtcc
     1081 cggccggacg ctgccccagg ctctcctcgc tggttgccta ctcgtcacga ccgtctacgt
     1141 gctcgccaac gtggcctatc tgaacgtcct gccgctccac gcggtgcagc acgcgcccgc
     1201 cgatcgggtg gcgaccgcag ccgcgacatc tctcttcgga cccgcggcag ccacggtgat
     1261 ggcgctcgcg atcatgattt cgacgttcgg atgcgtgaac ggtctcatcc tttcgggagc
     1321 gcgcgtctcg ttcgcgatgg cccgcgacgg cctgttcttc cagagcctcg agcgtttgaa
     1381 ccgggcctcc gttcccgcga acgccctctg ggcccaggcg ctgtgggcgt cggtcctcac
     1441 gatctccggc agctacagcg acctgctcga gtacgtgatt tctgccgaca tcctcttcta
     1501 cgtcctgctc gtcgtcgcgg tgatcgtgct ccggaggaag aggccggacc tgccgcggcc
     1561 ctttcgcgct ccgggttatc cggttcttcc gctgctatat gccgccgcgg gcgccgtcct
     1621 gatcggagtg ctgctcgcgg ggaacccgcg cacgacgtgg cctggctacg ggctcgtgct
     1681 cactggcgtt cccgtctact tcgcgtggcg ccgccggccg atgcagacgt gacggccgtg
     1741 aacctcgaag tgcatgacgt ctcggaagat tccgtcctcg tggagttccc cgaggcgccg
     1801 gacgccgaag cgaatcgggc ggcaatcgag atcggcgcgc ggctgacggc gaagcgccgt
     1861 cacggcctcg tcgacgcgat tcccggcgcc cgcacgctct atctggcctt cgaccaccgc
     1921 gtgatcgatc gcgaggcggt cgtgcgacgc gtgcgacgcg cggcggacga accggcggag
     1981 acttcttccg attcgcggct cctccggatc cccgtcgcct acggcggaga caacgggccc
     2041 gacctcgcga cgctcgcgcg ggatctcgga attgatccgg aagaggtcgc gcggcggcac
     2101 gagaacgcgg agtatcgcgt cgcgtttctc ggattctcgc cgggcttcgc ttacctgatc
     2161 ggactccccg tcgagctcgc gacgccccgc cgcgacagtc cgcgcccgcg cgtcgccgcg
     2221 ggtagcgtcg cgatcggggg cccgtacacg gcggtctatc cgacggaatc acccggcggc
     2281 tggcgcctga tcggacgttc ccccgtcggg ctattcgatc ccttcgcgag cccgccggcg
     2341 ctcctgcgac ccggcgatcg cgtgcggttc gagcggatcg acgaggacga gctcgcccgc
     2401 cggatcgcgg cgtcgatgga gcatccgcgg gcagccgcgg tctcggaggg agagccggtg
     2461 ttcgccgcga tcgcgcccgg gcttttctcg agcgtccagg gaggaccgcg ccacggcctc
     2521 ggcgcctatg gtgtgccggc gggcggggcc atggacgcga ccgcgctcga gcgcggcaac
     2581 gcgatcgtcg gcaacccgcc gcaggcgccg gctctcgagg tcacgctttc gggtccggag
     2641 ctccgcgtct tggacgacgc tctcgcgtgc gtcgccggcg gggacttcca ggcgcgatgc
     2701 aacggaaagc tcctcgagat cgacgcttcc ttcgagctga aggccggaga ccacctggcg
     2761 ttcggatatg cgcgaagcgg agcgcgggcg tatctctgcg tccggggcgg gctcgtcgag
     2821 cggcgcccgg gagagccgct gaagcgcctc gtgcgcggcg acatcgtcgc gagaggtcgc
     2881 ctggcggagc gcgcgcaggc tgcgctgccc cggaggcccg atgccgccgg cgacggcgtc
     2941 cgcgtccttc gcgttctcgc ggggcctcag ttcgcgttgt ttcccccggc cgcgacggac
     3001 gtcttttttt cggcgagcta ccgggtttct ccggaaagcg accggcgggg aatccggctg
     3061 gacggccccg tagtcgcatt gcgccgcctc tccgatatcc cccccgaagg gaccgcgctc
     3121 ggcgccatcc aggtcccggc gggcggactt ccgatcgtgc tcggccccga ccggccggtg
     3181 accggcggat acgcgaagat cgcgaccgtg atccgccgcg actggcccct tcttgcgcag
     3241 gcgcccccgg ggaccgcggt gcggtttcga gcggtgacgc tcgcggaagc aatcgagctg
     3301 atcggggaac ccttggacta acatcagggt tcttgagcat cgacctgaac gcggacgtgg
     3361 gcgaaggaat ggatgacggg gagctcctgc cgtacgtgac atctgcgaac gtcgcctgtg
     3421 gaatgcacgc gggcgacccc gtcacgatgg accagacggt ggagctcgcg ctctcccgcg
     3481 gcgtgcgcgt gggcgcccac cccgggtatc cggatcgcgc gaatttcgga cgcgtgatga
     3541 tggagatgtc cgcggacgag atcgagagcc tcatcgtgta ccaggtctcc gctctcgccg
     3601 gcttcgcgcg gtctcgcggg agccgactga ctcacgtgaa gcctcacggc gcgctgtatc
     3661 acagcggcgc ggagttcccg gacgtcgccc gcgcgatcgc ggagggcgtt cgccgggtcg
     3721 ggaccgatct cgtgctcgtc ggcgcggccg ggtcgatgct gatcggcgcc gggcaggaag
     3781 ccgggttgcc ggtcgcggag gaggcgttcg ccgatcgccg gtatcgtgcc gacggaactc
     3841 tcgtcccgcg gggccaccgg ggggccctca tcaccgaccc cgacgaggcg gccgaacagg
     3901 cggtcggcct cgcgcgcgac ggcgcggcgg tcgccgacga ctcatcccgg gtctccgtcc
     3961 gcgcggatac catctgcctt cacggcgaca cgcccggcgc cgcggaaatc gcgcgaagga
     4021 tccacgatcg gttccgcgcc gccggaatac ggatcgcgcc gctcgaaacc ggagtgaagc
     4081 gacgcgaccc tggcggcacc gcttgagcgg ccacgccgct cttccaggct gatgacgacc
     4141 gagtcggacg acttctccgc cgatctcccc gagccgggct ggatggaacg gctcgccgcg
     4201 atccaccgcg ccattcatgc cgtgctggcg acgcgcgccg agatcttccg cgaggagctc
     4261 gccgagaaag gatcgctgtt cgggaaaggg ctcgtcggaa tcgcgctcgc gctcgcgttc
     4321 gcggggctgt ctctcctcgt tctgaccgca ctcgtcgccg cgctcctcgc gaaactcttc
     4381 ggcggcccga tcgccggcat cacagccacc ctcgttctct atctcctgat cacggcttcg
     4441 gccgcgctct tcggaatcaa gaagctctcg cgcgtgaagc ctctcgactt tccggcgacg
     4501 cgggacgagc tgcgcaagga cctcgacgcc ctgcgcaagg aacctccgga gagcgacgaa
     4561 ggggcggcga gctccgacgc tctcgaggcg gagaaggcga gcgtccggcc gggcgaagcc
     4621 ggaggcggcg accgcgcaat cgcagaggac ctcgaggatc gcttccgggc gggttcggaa
     4681 tgaccgacga aaagacgaca cgacgtcgcg gcagtcgagc cgagcgcatc gaggagctgc
     4741 ggaggcgggg ggcattcgag agggacgctc tcacgcgcgg gatcgcgggg ttgcgcgggg
     4801 cgatcgacga gaagaaggcg ctctggaaga ccgtcggctg gatcgtcggg atcgccgcgg
     4861 cggcggggac cgtcggttac aagctcttcg gaaagcactc gctctcggcg aagatcgggc
     4921 gcgtgacgaa cgtggcgatg attctgttcg gactcggaaa ggcggccgga aaggcgcgga
     4981 agcttctgta gcccgcggct ctgccgtggg atcggaccac tgttctctga cgacgccgtt
     5041 ctgacctgga gagcggacat cccgctttcg ccttgatccg ctaagtcctt tcgaaccagt
     5101 cacctccctc tggcacccgc gttgccctct gccttccgtg tccgcgggga ggacgcgggc
     5161 gggaggtgag gatgaaaaga gcgattcgct tcgcgatggt ggctgccgcg atggcggcag
     5221 tgctcgtggt cttgacgccg tcggcggcgc aggcgcaggt ccgggtcgag ggccgtttcc
     5281 gcctcccgca cggccagatc gcgatcgggg tgggcgaccc ctattacggc cgaggttatt
     5341 acggcgacat ttattacggc gcgccggcgt atccggtcgg ctcgtacgtg ccgtacggct
     5401 accgggtgat cgaagatccg aggctcggtt acggcttcta cagcccggcg ttcgggtgca
     5461 gcctgcacca cgtccgtcat cggcactgga ttccggttcg ccgctcgacg aggcgctggg
     5521 tcgtgatcga acggccgtat ttcggcggcc gcgactatcg gcgtggctac gacagccggc
     5581 gatacaacga ccggcgatac aacgaccgcc gctacgacca gggctatcgc ggcgatccct
     5641 attacgactt caaccgcgag tactgggacc gcagctacca cccgtacgac gaccccaact
     5701 acgggcgccg tccctacgat cgtcggtaac gggacttttg aattaggaac tcgacctgag
     5761 tcagccgggt actcctcctt cccggcaggc cgctccgatt cccggggcgg ctttttttta
     5821 gaaggtcaga gtctggatca cgcgatcggc aatcgcccgg tagccctggc gattcggatg
     5881 ataccggtcc gtggcgaggc ggtcgggacg gccctgaaac aggtcgaaga cgggaaccgc
     5941 cgcgacgttc gcatacccga gagccgtctc cgcgaggacc gagttccacg agagcacgag
     6001 cgactcgccg acgcgcgact cccgtccctc tccgccgaag ggctgataga gcccgagcag
     6061 gcggatcggc gccgaagggt tcgcctcgcg taagtcccgc aggatcgacc gcaggttgtc
     6121 cgcgtagcgc gagcgcgaat cgccgacgct cgagagcgcc tgcgctggcg cgctcgcggc
     6181 cgagagcgag tgcgaaaggt cgtttcctcc caccgagaag aggatcaagt cggctgatgc
     6241 cgcgagcgac cggacgtttg cgctctcggc gagctttcga aggtcggagg actccgcgcc
     6301 cgcgacggcg aggttcaccg actcgaccgg cccgcgcctc tcgagcgcag ccacgacgtc
     6361 ggcggcgtat ccccggcccg tcgggtcgcc cgtgccgtgc gagagcgagt cgccgaggat
     6421 caggacgagg cgtttcccgg gatttttcgc tgcttgaagg ggagcggggg cggggggagc
     6481 ggccccgatc gggcttcccg aagagctccg gatcgcgagc cagaaaccgg cggtgaagac
     6541 ggcgccggca acgagcgcga gcgctgcggg aactcgccag agccagaacc gggagtagcg
     6601 catcgagccg gatgatacgt tcctcgaccg aggactgggg actggggact gacgtgggac
     6661 cgacgccgca caacgacacg atcgttctct ccgccgaagg cctgacgaag agctttggtc
     6721 ggcgcgaggt cgtctccgac gtctcgttcg agctccgcgc cggagaggtc ttcggctttc
     6781 tcggtccgaa cggcgccggc aagacgacaa cgatacggat gctcgtcggg ctcgcgcgcc
     6841 ccgaccgcgg ccgggtgcgc atcgccggct tcgacctcca gcacgacttc gcgaaagcaa
     6901 tggcgcgggt cggctgcatc gtcgagtcga ccgacctgta cccctacctg accgggcgag
     6961 agaacctgat ccacttcgcc cggatgctcc ccgacggcgc cgagaagcgc atccccgagc
     7021 tcgcgcgcct cgtgttcctc gaggcacggc tcgacgatcg cgtcgcgacc tattcgctcg
     7081 gcatgcgcca gcggctcggc ctcgcgcagg ctctcctcgg ggagcccgat ctcctcatcc
     7141 tggacgagcc cgcgaacggc ctggatcccg ccgggatccg ggagatccgg gagctcgtcc
     7201 gccacctcgc ccatgatcgg ggcatggcgg tgttcgtttc gagccacctg ctctccgaag
     7261 tcgagcagac gtgcgaccgc gtcgcgatca tccaccgcgg gcggacgctc gcgatgggac
     7321 cggtgagaga gctgctggaa tcgcgcggcg cggagcgcct cgtcttccgc gcgagcccct
     7381 ctgcccgcgc cgaggaagtc ctcgcgccgt tcgggcgcgc cggagcgtcg cggatcgagg
     7441 gggaggaagc ggtttcggcc gacgtgccgc gcgaaagagt gccggaagcg ctcgcggccc
     7501 tcgcggcggc cggcgtcctc gtctacgccg tcgaacgaca gtcgtcgacg ctcgaggaga
     7561 tcttcctcga ggtcacggga ggggaaacgg tgtgaggtgg ccgccatgaa gttcgcgccg
     7621 ctcgtcgaga acgagatcct gaagctcctg aagcgccgcc ggtttcgggt cgcgctgctg
     7681 atcctggtcg cgttgaacgg tctcatcgtc ttcgcgcaga cgcagagccg ggaccggcgt
     7741 ccgcggggcg actggaggat cgaagcgcag gagcgcgtcg cccggatgca gaactgggag
     7801 cgctcgggcc ggctgccgca gacgcagttg aagtgggtcc ggttcgaaat cgcccgcgtg
     7861 cagtaccacc tcgatcgcaa catggacccc gaggcgatct ccggtccgct cttcgcgcgc
     7921 gcgttcgcga acgcgtcgag ttaccttctc ctgcccctcc tggcaatcct cttcgccgcc
     7981 gacatcgtgt cctccgagtt cgcgcaggga acgatcaaac tcctgctcac gaggccggtc
     8041 ggacgcgcgc gggttctcac ctcgaagttc gccgcgctcg cggtcgcgat caccctgacc
     8101 gtcctcctgg gcggcgtcgt cgcttacctc ttcggaggcc tggcattcgg ttatcgaggg
     8161 tggggcgcgc ccgtcctgac cggtttccgg ctgtccggcg agaccttcga tccgaccgcc
     8221 gtgcgcgcgg ttccgctctg ggaggacgca ctcctcgcgt tcggcctcgc ctggtacgcg
     8281 gcgctgaccg tcggcgcgat cgccttcctg acgtcg
//
LOCUS       DASWAV010000270         1744 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U2665
            k141_773318, whole genome shotgun sequence.
ACCESSION   DASWAV010000270 DASWAV010000000
VERSION     DASWAV010000270.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960316
            Sequence Read Archive: SRR10260007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 1744)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1744)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:47:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,929
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1744
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_773318"
                     /isolate="SMAG_U2665"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.36444 N 132.31889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1123)
                     /locus_tag="VF958_05590"
     CDS             complement(<1..1123)
                     /locus_tag="VF958_05590"
                     /inference="COORDINATES: protein motif:HMM:NF012311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HEX9148619.1"
                     /translation="MPRRQLTGFAIVAVFTLFAGRARALRPISPDEAPKADARLLRAL
                     NEGSEEIRVIVGVRDGTPSAHALLLAPDPAGEPARRARRLVAQKRLSDDLPPNEFRVR
                     HFYESFSLLAGRATREGVVRLANRPDVEWVTVDQTRRRFQASPQAAASLIRSDQANAS
                     GFTGAGQTVAVIDTGVDYTHPQLGGGTFPNAKVIGGMDAADEDNDPMDCEGHGTSVSG
                     VIAGPAGVAPDAKIVALKVFKSNDATNATCSDTAFVSDIIQAINYAISHKAEFHIGVI
                     NMSLGAEPDSDDLGYCDQSDREEAVALDAAAAADIPVVVASGNGLHLHALASPACISS
                     AVSVGAVYSLSSALVRWSDCTDQPVTPDLVTCFSNSNTNL"
     gene            1252..>1744
                     /locus_tag="VF958_05595"
     CDS             1252..>1744
                     /locus_tag="VF958_05595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HEX9148620.1"
                     /translation="MRAVFADCHVGRRPDDDGPFLAALEEAAGRGARAISLLGDIFHF
                     FIAHPKFETPPIARFLEAVDRLRVAGIPVTYVEGNRDFFLRGSYVEPRFRKVCDEETF
                     QEGGRRFLLTHGDLLNEEDRPYRLWRAISKNRISRAAIDFLPRKAANRFVWKVEARLY
                     RSNF"
BASE COUNT          284 a          553 c          608 g          299 t
ORIGIN      
        1 agaggttcgt gttcgagttg gagaagcagg tcacgaggtc cggagtcacc ggttgatcgg
       61 tgcagtcact ccagcggacg agtgccgagg agagcgaata caccgcgccg accgagacgg
      121 ccgacgagat gcaggcgggc gaggcgagcg cgtgcaggtg gaggccgttg ccggaagcga
      181 cgacgaccgg gatgtcggcc gcagcggcag cgtcgagcgc aacggcctcc tcccgatcgg
      241 actggtcgca gtagccgagg tcgtcgctgt ccggctcggc gccgaggctc atgttgatga
      301 cgccgatgtg gaattccgcc ttgtgggaga tcgcgtagtt gatcgcctgg atgatgtcgg
      361 agacgaacgc ggtatcgctg caggtcgcgt tcgtcgcgtc gttcgacttg aagaccttga
      421 gcgcgacgat cttcgcgtcc ggggcaaccc cggcgggtcc ggcgatgacc ccggagaccg
      481 acgttccgtg gccttcgcag tccatcgggt cgttgtcctc gtcggcagcg tccattccgc
      541 cgatgacctt cgcattgggg aaagtcccgc cgcccaactg ggggtgcgtg taatcgacgc
      601 cggtgtcgat gaccgcgacg gtctgcccgg cgcccgtgaa tcccgaggcg ttcgcctggt
      661 cgctccggat caacgaggcc gccgcctgcg ggctcgcctg gaagcggcgc cgggtctggt
      721 cgaccgtgac ccactcgacg tcgggccggt tcgcgaggcg cacgactccc tcccgcgtcg
      781 cgcgtccggc aagaagtgag aagctctcgt agaagtgcct cacgcggaac tcgttcggcg
      841 gaagatcgtc ggacaggcgc ttctgggcga ccagacgccg ggcgcggcgg gcgggctccc
      901 cggccgggtc gggcgcgagg agaagcgcgt gggcggacgg cgtcccgtcc cggacgccga
      961 cgatgacccg aatttcttcg gagccctcgt tcagcgctcg aagaagcctg gcgtcggcct
     1021 tgggggcctc atcgggcgat atcgggcgca gagcgcgggc ccggccggcg aaaagggtga
     1081 aaacggcgac gatcgcaaac ccggtcaact ggcggcgcgg catgtatccc ccgaaagagt
     1141 tttacggttt gacgaaacag aatacgtccc gaagtccggg aaaaatcccg tttggaccgt
     1201 tccggtgcgc aaaatcctcg tttcgcagcc gttgggctat ctttccggcc gatgagggcc
     1261 gtatttgccg actgtcacgt ggggcgccgc ccggacgacg acggaccctt tctggcggct
     1321 ctcgaggagg cggccggccg cggggcgcga gccatttcgc tcctggggga catttttcac
     1381 ttcttcatcg ctcatcccaa gttcgagacc cccccgatcg cccgattcct ggaggccgtc
     1441 gaccggctgc gcgtggccgg aattccggtg acctacgtcg aaggaaaccg cgatttcttc
     1501 cttcgcggct cctacgtcga gccccggttc cggaaagtgt gcgacgagga gacgttccag
     1561 gagggggggc gtcggttcct cctgacccat ggcgacctgc tgaacgagga ggaccgcccg
     1621 taccgactct ggcgcgcgat ctcgaagaat cgcatctcgc gcgccgcgat cgacttcctc
     1681 ccgcggaaag cggcgaaccg tttcgtttgg aaagtcgagg cgcgactcta ccggtccaac
     1741 ttca
//
LOCUS       DASWAV010000271         2332 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U2665
            k141_136308, whole genome shotgun sequence.
ACCESSION   DASWAV010000271 DASWAV010000000
VERSION     DASWAV010000271.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960316
            Sequence Read Archive: SRR10260007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2332)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2332)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:47:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,929
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2332
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_136308"
                     /isolate="SMAG_U2665"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.36444 N 132.31889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..610)
                     /locus_tag="VF958_05600"
     CDS             complement(<1..610)
                     /locus_tag="VF958_05600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9148621.1"
                     /translation="MTLTKWTKRWLAALAIAFALIVIDGGEANRLGFLGALAWMTFVA
                     ACLVLGFRVIRSAFRLVVRRLTLRLAFSYFLIGIVPIPLLATLAFVLFYVVAHQLVAT
                     RVWHEATEMASTATGLPGVQVRDGKVVRSEVSWLEPGNPAPWAAQVDRPRAVIEGKNV
                     WIAVRDERDADSVRLLYLNDPSGAWLKKLADRTGYAVRVETGR"
     gene            646..1149
                     /locus_tag="VF958_05605"
     CDS             646..1149
                     /locus_tag="VF958_05605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9148622.1"
                     /translation="MRFFRHSALLLPSAFLLGVLLFLLTHGREDPGFAAYPFFEEWGA
                     SSSQTPEFRFLEQSLVFFAPVYLIMLLFVLLLAVAENALYGPRRPVARSIYDRAFRKI
                     FPALYMITVAAIVLAGDRLARAYAPGALVGPVLVALTPFAAALFALVPALLFAAPAAV
                     LMRMERA"
     gene            1146..>2332
                     /gene="guaA"
                     /locus_tag="VF958_05610"
     CDS             1146..>2332
                     /gene="guaA"
                     /locus_tag="VF958_05610"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: protein motif:HMM:NF000848.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HEX9148623.1"
                     /translation="MSAHGRSIVVLDFGGQYTQLIARRVRDARVFSVVLPPDATEEAI
                     RKFEPAGIILSGGPQSVYEEGAPLPKADPQALGVPVLGLCYGMQWMAHALGGEVKPSN
                     GRGEFGRATARLVTDSELFAGLDPEQTVWMSHGDSVAKPPPGFRVIGSTPATPVAAIE
                     DPARRLYGIQFHPEVRHTEHGTAILENFLFRACGVKPDWTMESFRHEKVEAIRRQAPS
                     GAVICALSGGVDSSVTALLLREAVGDRMHPILVDHGLMRAHETQQVEEAFGRLGIAIR
                     SVDASDLFLSRLEGVEDPEEKRRIIGRTFIEVFEREARGIPDARYLAQGTLYPDVIES
                     VSIKGPSAVIKTHHNVGGLPEKLGLELIEPVRELFKDEVRRLGAELGLPREFLSRHPF
                     PGPG"
BASE COUNT          348 a          787 c          806 g          391 t
ORIGIN      
        1 cgcggccggt ctccaccctc accgcgtatc ccgtgcggtc ggccagcttc ttcaaccagg
       61 ccccggacgg gtcgttcagg tagaggagcc ggacgctgtc ggcgtcacgc tcgtcgcgga
      121 ccgcgatcca gacgttcttt ccttcgatga ccgcccgggg gcggtcgacc tgcgcggccc
      181 acggcgctgg atttcccggc tcgagccacg agacctcgga gcgaacgact tttccgtccc
      241 ggacctggac gccgggcaga ccagtggcgg tcgaggccat ctcggtggcc tcgtgccaga
      301 cgcgcgttgc cacgagctgg tgggcgacga cgtagaacaa gacgaaggcg agggtcgcca
      361 acaggggaat cggaacgatc ccgatcagga agtacgagaa tgcgaggcgc agcgtgaggc
      421 gccggacgac gagtcggaac gcagaacgga tcacccggaa gccgaggacg aggcacgcgg
      481 cgacgaacgt catccaggcg agggctccga gaaagcccag ccggttcgcc tcgccgccgt
      541 cgatcacgat gagggcgaag gcgatggcga gagctgcgag ccaccgtttc gtccacttgg
      601 tgagggtcac gcgaggcgaa ggataccgtc gtagaatggc cgcgggtgag gttcttccgg
      661 cattcggcgc ttctccttcc gtccgcgttc ctcctgggcg tcctcctctt cctgctgacg
      721 cacggccgcg aggacccggg ctttgcggcc tacccgtttt tcgaagagtg gggggcgagc
      781 agcagccaga ctcccgagtt ccgctttctt gagcagtcac tcgtgttctt cgcgccggtc
      841 tacctcatca tgcttctttt cgtactgctc ctcgcggttg cagagaacgc tctgtatggc
      901 ccacggcgcc ccgtcgcgcg ctcgatctat gaccgcgcgt tccggaagat cttcccggcg
      961 ctttacatga tcacggtggc ggcgatcgtc ctcgccgggg accggctcgc gcgcgcgtat
     1021 gcgcccgggg cgctcgtcgg cccggtgctc gtcgcgctaa ccccctttgc ggccgctctc
     1081 ttcgcgctcg tccccgcgct cctgttcgcg gcaccggccg cggtactgat gcgcatggag
     1141 cgggcgtgag cgcgcacggg cgctcgatcg tcgtcctcga cttcggcgga cagtacacgc
     1201 agctgatcgc ccgccgcgtt cgggacgcgc gggtcttctc cgtggttctc cctcccgacg
     1261 cgaccgagga ggcgattcgg aagttcgagc cggcggggat catcctctcc ggcggccccc
     1321 agagcgtgta cgaggagggc gcgccgctgc cgaaggcgga tccgcaggcg ctcggcgtcc
     1381 cggtgctcgg tctctgctac ggaatgcagt ggatggcgca cgcgctcggc ggagaagtca
     1441 agccgtcgaa cggacgcggc gaattcggcc gcgcgaccgc gcgcctcgtc acagactcgg
     1501 agctcttcgc cggtttggac cccgagcaga cggtatggat gagccacggc gactcggtcg
     1561 cgaagccgcc accggggttt cgggtcatcg gctcgacgcc ggcgacgccc gtcgcggcga
     1621 tcgaggaccc ggcgcggcgc ctctacggga tccagttcca tcccgaggtc cgccacaccg
     1681 agcacggcac ggcgatcctg gagaacttcc tcttccgcgc ctgcggcgtc aaacccgatt
     1741 ggacgatgga gtccttccga cacgagaagg tcgaggcgat ccgccggcag gcgccgtccg
     1801 gcgccgtcat ctgcgctctc tccggcggcg tggattcctc ggtgaccgct ctcctgctgc
     1861 gcgaagcggt gggagaccgc atgcatccga tcctggtgga ccacggcctc atgcgcgcgc
     1921 acgagacgca gcaggtcgag gaggctttcg ggcggctcgg cattgccatc cgttcggtcg
     1981 acgcgtccga tctcttcctg tcgcggctcg aaggcgtgga agatcccgaa gagaagcgcc
     2041 ggatcatcgg gcggacgttc atcgaagtgt tcgagcgcga ggcgaggggg atcccggacg
     2101 cgcgctacct cgcgcagggg actctctacc cggatgtgat cgagtcggtc tcgatcaagg
     2161 ggccgtcggc ggtcatcaag acgcaccaca acgtcggcgg tctgccggag aagctcgggc
     2221 tggaattgat cgagccggtg cgcgagctct tcaaggacga ggtccgacgg ctgggcgcgg
     2281 agctcgggtt gccgcgggag ttcctctcgc gccacccctt tcccggaccg gg
//
LOCUS       DASWAV010000272         3088 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U2665
            k141_773487, whole genome shotgun sequence.
ACCESSION   DASWAV010000272 DASWAV010000000
VERSION     DASWAV010000272.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960316
            Sequence Read Archive: SRR10260007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 3088)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3088)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:47:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,929
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3088
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_773487"
                     /isolate="SMAG_U2665"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.36444 N 132.31889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            192..1574
                     /locus_tag="VF958_05615"
     CDS             192..1574
                     /locus_tag="VF958_05615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HEX9148624.1"
                     /translation="MTEELDRKALSLFTERERGRYEDLLTELVETPTVSSEVSHRADI
                     DAGVEIVRRILTGAGGRVTVAKTKGNPVVLGVLPGRAGAPSVTVYHHIDVQPASRETE
                     PWETDPFRLVKTDGRYYGRGSTDDKGPALCAFFGALAAREAGVPVEIRFLWEFEEEIG
                     SPNFEEGLRALGDAARADSVLVGDGLWLDSRHPAATSGLRGMQSFQFVLETGRNETHS
                     GTTGGAARNPIGELAQLVSEIYDAKTGRVKIPGFSDDVVPLSRRDAEEFRRSGFSLQT
                     FKKDFGFKSLRSKDTLDVLKRLWAQPTFEIHGVTGGYTGEGVKAIIPNRAEFKVSCRL
                     VPDQKPEKIARLVRDFVKRRNPDVEVIVGGSAEPFRGETTGPYAEAVKRAMQFAFGKP
                     AAFIRAGGSIGAVVTMKKVLRRPIAFLDVSLPEHGYHAPNEHFDWGQASGGIAAYAKY
                     FEELARRTSA"
     gene            1571..2077
                     /locus_tag="VF958_05620"
     CDS             1571..2077
                     /locus_tag="VF958_05620"
                     /inference="COORDINATES: protein motif:HMM:NF024273.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HEX9148625.1"
                     /translation="MKAGGELALLLRILDEAYEKRAWHGPNLKGSLRGLGAQEASWRP
                     GPDRHNVWEIAVHAAYWKYAIWRRLTGEKRGAFPLEGSNWFARPDVPTDKAWRADVAM
                     LEDQHRRLRATVAKLSPTALGVKPHGSKHPADTLVYGGAAHDVYHAGQIQLLKRLFRD
                     RGARAVRV"
     gene            2070..2402
                     /locus_tag="VF958_05625"
     CDS             2070..2402
                     /locus_tag="VF958_05625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9148626.1"
                     /translation="MSDAPLACRLDALDPSQRKRHALLARELGEAVTNVEELPDGLAI
                     RFPSKPYVFLRLAEWMELERACCPFLEFGLIFENRVPTMRLELRGPEGVKDFLQAELP
                     FAPAPTTP"
     gene            2399..3085
                     /locus_tag="VF958_05630"
     CDS             2399..3085
                     /locus_tag="VF958_05630"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012889296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016812 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in cyclic amides
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase family protein"
                     /protein_id="HEX9148627.1"
                     /translation="MTRLIDLSHEIEPGMVTYPGLPGPVVSDFLSREASRGKYAEGTT
                     FAIARVDMVANTGTYVDAPFHRFVAGKDVGSLPLDKLADLEGVVVDATRAGRTIGPEL
                     FDGLDLRGKAVLVNTGWSRHWRTAAYLDGHPYLTRPAVERLVAARPALVGIDSLNIDD
                     TRDGTRPAHTLLLESEVPVVEHLTNLEELPARGFRFHCAPAPFHGLGTFPVRAYAVLD
                     DDAGGLSARR"
BASE COUNT          506 a         1056 c         1052 g          474 t
ORIGIN      
        1 aaccgcggcg cgcagattct gaggcgaatc gattgagacg tcgccgagtt cgtcgtcgtc
       61 tccttctggg acggttacga tgcgatctgc cgcttcgcgg gcgacgaccc ggctcgcgcg
      121 ttctactatc cggaagatga cgaattcctc ctggagaagg agcccaccgt gacgcactac
      181 gaggtcttcg aatgacggaa gagctcgatc ggaaagccct ctcgctcttt accgaacggg
      241 agcggggccg ctacgaagat cttctgactg agctcgtcga gaccccgacc gtctcctcgg
      301 aggtgagcca ccgggccgac atcgacgcag gggtggagat cgtccggagg atcctgaccg
      361 gcgccggagg gcgggtgacg gtcgcgaaga cgaaagggaa cccggtcgtc ctcggcgtcc
      421 ttcccgggag agccggagct ccgtcggtca ccgtttacca ccacatcgac gtccagcccg
      481 cgtcgcgcga gaccgagccg tgggagaccg atccttttcg cctggtgaag acggacggcc
      541 gctactacgg acgcggctcg accgacgaca aggggcccgc tctctgcgcg ttcttcggcg
      601 ccctggcggc acgcgaagcg ggagtccccg tcgagatccg cttcctgtgg gagttcgagg
      661 aggaaatcgg ttcaccgaac ttcgaggagg gactccgcgc gttgggagac gcggcgcggg
      721 cggattcggt cctcgtcggc gacgggctct ggctcgacag ccgccacccc gcggcgacgt
      781 cgggactgcg cggcatgcag agctttcaat tcgtgctcga gacgggccgg aacgagactc
      841 actccggcac gaccggcggc gccgcccgaa acccgatcgg ggagctcgcg cagctcgtct
      901 ccgagatcta cgacgcgaag acgggccgcg tgaagattcc cggcttctcc gacgacgtcg
      961 tgccgctctc gcgccgcgac gcggaggagt tccggcggag cggcttctcg ctccagacct
     1021 tcaagaagga cttcggcttc aagtcgctcc gctcgaagga cacgctggac gttctgaagc
     1081 gcctctgggc gcaacccacg ttcgagatcc acggcgtgac cggcggttac accggagaag
     1141 gggtcaaggc gatcattccc aaccgcgccg agttcaaggt ctcttgccgg ctggtgccgg
     1201 accagaaacc cgagaagatc gcgcggctcg tgcgcgactt cgtcaagcgc cggaaccccg
     1261 acgtggaggt gatcgtcggc gggagcgcgg agccgttccg cggcgagacg accggcccct
     1321 acgccgaggc ggtgaagcgc gcgatgcagt tcgccttcgg aaagccggcg gccttcatcc
     1381 gagcgggcgg atccatcggc gcggtcgtca cgatgaagaa ggtgctgcgc cgccccatcg
     1441 cgttcctcga cgtgtccctc ccggagcacg gctaccacgc tcccaacgag cacttcgact
     1501 ggggacaggc gagcggcggg atcgccgcgt acgcgaaata cttcgaggag ctcgcgcgga
     1561 ggacgtcggc gtgaaggccg ggggcgagct cgcgctgctc ctgcggattc tcgacgaggc
     1621 gtacgagaaa cgggcctggc acggtcccaa cctgaagggc tcgctgcgcg ggctcggcgc
     1681 gcaggaggcg tcctggcggc cgggaccgga tcgccacaac gtctgggaga tcgcggtaca
     1741 tgcggcctac tggaagtacg cgatctggag gcgcctgacc ggcgagaagc gaggggcatt
     1801 tccgctcgaa ggcagcaact ggttcgcgcg gccggacgtt ccgaccgaca aggcctggcg
     1861 cgccgacgtc gccatgctcg aagaccagca tcggcgcctg cgggcgacgg tcgcgaaact
     1921 ttctccgacg gcgctcggag tgaagccgca cggcagcaag cacccggccg acacgctcgt
     1981 ctacgggggc gcggcgcacg acgtttacca cgccggccag atccaattgt tgaagcgcct
     2041 cttccgcgac cgcggcgcga gagcggtccg tgtctgacgc gccgctcgcc tgccggctcg
     2101 acgcactcga cccctcgcag cgaaagcggc acgccctcct cgcgcgcgag ctcggggaag
     2161 cggtcaccaa cgtcgaggag ctcccggacg ggctcgcgat ccgcttcccc tcgaagccct
     2221 acgtctttct ccgcctcgcc gagtggatgg agctcgagcg cgcgtgctgc ccgtttctcg
     2281 agttcgggct gatcttcgag aaccgcgtcc cgacgatgcg gctcgagctg cgcggaccgg
     2341 aaggcgtgaa ggatttcctc caggccgagt tgccgttcgc gccggcgccg acgactccat
     2401 gacccgtctg atcgacctga gccacgaaat cgagcccggc atggtgacct atccggggct
     2461 gccgggtccg gtcgtctcgg attttctctc gcgggaagcg tcgcgcggca agtacgccga
     2521 gggcacgacc ttcgcgatcg cccgcgtcga tatggtcgcc aacacgggaa cctacgtcga
     2581 cgcgccgttc caccgtttcg tcgcggggaa ggacgtcggc tcgctgccgc tcgataagct
     2641 cgcggacctc gaaggagtcg tcgtcgacgc gacgcgggcg ggccgaacga tcgggccgga
     2701 gctgttcgac ggcctcgacc tccggggcaa agcggtcctg gtgaacaccg gctggagccg
     2761 ccactggaga acggcggcgt atctcgatgg ccatccctat ctcacgcgtc ccgcggtcga
     2821 gcgtttagtc gcggcgcggc ccgcgctcgt cgggatcgat tcgctcaaca tcgacgacac
     2881 gcgcgacgga acgcggcccg cgcacaccct cctcctcgaa tcagaggtcc ctgtcgtcga
     2941 gcacctgacg aacctcgaag agctccccgc gcgcggcttc cgcttccact gcgcgccggc
     3001 gcccttccac gggctcggaa cttttcccgt gcgggcgtat gccgtgctcg acgacgacgc
     3061 cggcgggctc agcgcccgcc gctgaacc
//
LOCUS       DASWAV010000273         2495 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U2665
            k141_215290, whole genome shotgun sequence.
ACCESSION   DASWAV010000273 DASWAV010000000
VERSION     DASWAV010000273.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960316
            Sequence Read Archive: SRR10260007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2495)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2495)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:47:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,929
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2495
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_215290"
                     /isolate="SMAG_U2665"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.36444 N 132.31889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..245)
                     /locus_tag="VF958_05635"
     CDS             complement(<1..245)
                     /locus_tag="VF958_05635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008030201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HEX9148628.1"
                     /translation="MRFMVIVKADKDSEAGVMPSEELLAEMGRYNEELAKAGVLLAAE
                     GLHPSSKGARVRFSGSKRTVIDGPFAETKELIAGFWL"
     gene            569..>2495
                     /locus_tag="VF958_05640"
     CDS             569..>2495
                     /locus_tag="VF958_05640"
                     /inference="COORDINATES: protein motif:HMM:NF013736.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HEX9148629.1"
                     /translation="MSRALRIAVFGLVVVVTAATAGLAFYSFARKVATFRTAGFTWER
                     AGDALVVRSVEPESAAQAAGLRPGDRIVLADGRTAGSLEWPEKDLARRPLPHHLVVIS
                     GGAISAVALGEPAAQPDYTYIFLAFVGLLYLVIGLFTVVRERAPASRVFWAVCLSSFA
                     IYVITPAGPHDWIWKASWLTEDFFRALLPALLLHLFLIFPKPARSRRLVPLLYVPAAA
                     YMAAELSVLSMSGPSAARATETMTRLWFIYFVLYAGVVIGRLATLVSTRAVPAAEKQA
                     RWIALGVTVGLAPFVLLSALPRALGFASPLLSTIAVVPLVFIPLAFAWAILKWRLWDV
                     EIFVREAVAATGAVLIGGMTFVLLNALLDRTLVGMAEAGKNVIAFASGLVLASLLVPV
                     KRRLTDALEKIQYRETYRARRALLEVARDFSAPRPKEELVHAIVRRVEEGLHVVPCSL
                     FLFDDGGAENPEAEFLTARLDPTEIWRLRATAFGELEHPVARHLHAMGYRTFFAMKSG
                     GKLAGALGVGHKDGRVPLSSEDESLLRAVMAQAGLAYENARLYGALADRLEEIRALQQ
                     YQESVIQSSSSGIVVLDGADHVHSANPAFAQLVGREEADLVGLPFSDVLPGVEAGPAP
                     ADGGERTVEARFSAADET"
BASE COUNT          341 a          872 c          792 g          490 t
ORIGIN      
        1 atcagccaga agccggcgat caactccttc gtctcggcga aaggtccgtc gatcacggtg
       61 cgcttcgatc ccgaaaaccg gacccgcgca cccttcgagc tcggatggag tccctccgcc
      121 gcgagcagca cgcccgcctt cgccagctct tcgttgtacc ttcccatttc ggcgagcagc
      181 tcctcgctgg gcatgacgcc ggcctcggag tccttgtcgg ccttcacgat caccatgaat
      241 cgcatcttct gtctcctttc gagttcgagc ctccggttcg ggccgggccc cggtacggtc
      301 tcgaccgggc gtcacccgag ctcctgctac cacgacgaac ggacgggcac gagatcgacg
      361 cctcggaacg tcgtcttttt ttgatcgccc gactcaccgg cccgcgttcg caccctccga
      421 catccaaaat acttttgaat tgtgttcaaa tgattgaatg tcggcactcg atcgacggct
      481 tgctgacgag tcaagtcacg tcacaaaagc acttagcttg atgtccaata accccgatcg
      541 tggcattcgc attgctgaat tgccgcacgt gtcccgcgct ctgagaatcg cggtgttcgg
      601 cctcgtggtc gtggtcaccg cggccactgc gggactcgcg ttctactcgt tcgcccgaaa
      661 ggtcgcgact tttcgaaccg ccggcttcac gtgggaacgg gccggggacg ccctcgtggt
      721 ccgctcggtc gagccggaga gcgcggcgca ggccgcggga ctccgcccgg gcgaccggat
      781 cgtcctcgcc gacggccgga ccgcggggtc gctcgagtgg ccggaaaaag acctcgcccg
      841 ccgccctctt ccccatcacc tcgtcgtcat ctcgggcgga gcgatcagcg ccgttgccct
      901 gggtgagccg gcggcccagc cggattatac gtacatcttc ctcgcgttcg ttgggctgct
      961 ctacctggtc atcggcctct tcaccgtcgt ccgggagcgt gcgcccgcgt cccgcgtctt
     1021 ctgggcggtc tgcctctctt cgttcgcgat ctacgtgatc acgccggcgg ggccccacga
     1081 ctggatctgg aaggcctcgt ggctgacgga ggacttcttt cgcgcgcttc ttccggcgct
     1141 tctccttcac ctcttcctga tctttccgaa accggcccgc tcgcgccgac tcgttcccct
     1201 tctctacgtg ccggccgcgg cgtacatggc ggcggagctg tccgtactct cgatgtccgg
     1261 accttccgcg gcgcgagcga cggagacgat gacgcggctc tggttcatct acttcgtgct
     1321 ctacgcgggc gtcgtgatcg gacgactggc gactctcgtg tcgacgcgtg cagttcccgc
     1381 cgccgagaag caggcgcggt ggatcgcgct cggcgtcacg gtcggcctcg cgcccttcgt
     1441 cctgctgtcc gcgctgcctc gagcgctcgg gttcgcgtct cccctcctct cgacgatcgc
     1501 cgtcgtgccg ctcgtcttca tccccctcgc gttcgcgtgg gcgatcctga agtggcggct
     1561 ctgggacgtc gagatcttcg tgcgcgaggc ggtcgctgcg acgggggcgg tcctgatcgg
     1621 cgggatgacg tttgtcctcc tgaacgcgct cctcgaccgc acgctggtcg ggatggccga
     1681 ggcgggcaag aacgtgatcg cgttcgcctc gggtctcgtt ctcgcatcgc tgctcgtccc
     1741 cgtcaagcgg cgcctcaccg acgccctcga gaagatccag tatcgcgaga cataccgcgc
     1801 gcgccgagcg ctcctggaag tcgcgcgcga tttctccgcg cctcgtccga aggaggaact
     1861 cgttcacgcg atcgtccggc gcgtcgagga gggactccac gtcgtgccct gttccctctt
     1921 cctcttcgac gacggcggcg cggagaaccc cgaagccgag ttcttgaccg cgcggctcga
     1981 tccgacggag atctggcggc tgagggcgac cgccttcggc gagctcgagc acccggtggc
     2041 gcggcacctg cacgccatgg gataccggac gttcttcgcg atgaagtcgg gcggcaagct
     2101 ggccggcgct ctcggcgtcg gtcacaagga cgggcgggtt ccgctctcct ccgaggacga
     2161 gtcccttctg agggccgtga tggcacaggc cggcctggcc tacgagaacg cgcggctcta
     2221 tggggcgctc gcggaccggc tggaggagat ccgagcgctg cagcagtacc aggaaagcgt
     2281 gatccagtcg tcttcgtcgg ggatcgtcgt attggacggc gcggatcacg tccactcggc
     2341 gaatcctgcg ttcgcgcagc tcgtcggccg ggaagaggcc gatctcgtcg gcttgccttt
     2401 ctccgatgtt ctcccgggcg tcgaggccgg cccggctccc gcggacggcg gcgaaagaac
     2461 ggtcgaagcc cgtttctccg cggcggacga gaccg
//
LOCUS       DASWAV010000274         3762 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U2665
            k141_175205, whole genome shotgun sequence.
ACCESSION   DASWAV010000274 DASWAV010000000
VERSION     DASWAV010000274.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960316
            Sequence Read Archive: SRR10260007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 3762)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3762)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:47:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,929
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3762
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_175205"
                     /isolate="SMAG_U2665"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.36444 N 132.31889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..475
                     /locus_tag="VF958_05645"
     CDS             <1..475
                     /locus_tag="VF958_05645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9148630.1"
                     /translation="LADRQAGGSGFLRGEGLGRQIHRVPADASAWAIVDATRAPFPSG
                     ARRSEDGDAAEALVGAMKSVSLFVFHATARGDSVDLSATGITADDETRGLLEDALKGV
                     VAMWRLAIHEKSPELVPILRKFEIESDHEGVTIHGTLPGSFLRKMAEERHARRDD"
     gene            complement(572..1246)
                     /locus_tag="VF958_05650"
     CDS             complement(572..1246)
                     /locus_tag="VF958_05650"
                     /inference="COORDINATES: protein motif:HMM:NF012334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX9148631.1"
                     /translation="MGTWVVTGANRGIGLAFARALSKRGETVVGTARQPAGANELRAA
                     GARVEKLDVSKDSSVADFVRRLEGVPVDVLVHNAAVGEEGSRIEELEPDAVLRALDVN
                     AVGPERVTRALLPNLFAGRLRKVVAITSGLGSLSQNTDGGWYAYRMSKVALNMFVRTL
                     AEELAKERFTCAVLSPGWVRTAMGGPNAPLTPDESVAEMLKVIDGFRPSDAGRFLDRH
                     GRDVPW"
     gene            1320..3407
                     /locus_tag="VF958_05655"
     CDS             1320..3407
                     /locus_tag="VF958_05655"
                     /inference="COORDINATES: protein motif:HMM:NF013440.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GreA/GreB family elongation factor"
                     /protein_id="HEX9148632.1"
                     /translation="MSQAVSQLPPEIAALVDQKRLEELEDVWTRRMEQDPSDLQFFFR
                     IAAAVKKKAGAEGAEQALSWLRFLADYQSERGDPEARIEVLAEIVRMNPLDLSARADL
                     ERALAERYRDHPVLAAVMAQNPIANASDPSETARRVARWLRFLPGGVYYLAGRGAGRI
                     AEMNPALDVIRLEVGGARVPLSLVSAEKNLEPLPAGHFLREKVENLDGLRDLVSREPA
                     EAVRRLLSSFGRPLSVGEVREHFSGVVDDARWSSFWTSARRHPQLLVAGAAKSALVSW
                     SESADAADETVRREFESAEPQKKLEIARRHAKRSRELARLFARSLASEAGSAADRGDA
                     ALAWELSQAAARLAPGEPEAFPAESLLASSNVAAVLGSIRDHVAREKALEQVRARRDD
                     WPAIFAARFQEEEDARVLSALFEALGEDRSRRDELVRRVMRSPRIAPRAFVWLCERSG
                     ADASPDLSASALFHSLLDALRQDEFGSVRARVKAFFEPGALAVDLVKRVGTEEEARDF
                     VNALARAGGLEEHRRGTVREALLMRFPTLRAPAMNYLYATPEAIERRRNELAQLKQVE
                     LPANAEQMRAAKEHGDLSENFEYHAARQKHEYLSARIAKLADELSRTRALDSTRIDAS
                     EVRVGTRVVLREKESGRERIATVLGPWDSQPELQIYSYESEFATALLGKRPGETVTLP
                     DGTLEVVSISPWR"
     gene            3443..>3762
                     /locus_tag="VF958_05660"
     CDS             3443..>3762
                     /locus_tag="VF958_05660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="damage-inducible protein DinB"
                     /protein_id="HEX9148633.1"
                     /translation="MKSGVARVLPFLVLALSSVSSSAIAQAPAAASKASFGSEISKEL
                     ADVEKKLVTLAEAMPAEKFGWRPAEGVRSVSEVYVHVAGGNYMLPTMFGFKRPEGMVG
                     RDME"
BASE COUNT          586 a         1287 c         1305 g          584 t
ORIGIN      
        1 gctcgcggac cggcaggccg gcggctcggg cttcctccgc ggcgaagggc tgggaaggca
       61 gatccaccgc gtccccgccg atgcttccgc gtgggcgatc gtcgatgcga cgcgggcgcc
      121 gttcccgtca ggcgcgcggc gctccgagga cggcgacgcc gcggaagcgc tggtcggtgc
      181 gatgaagtcc gtgtcgctct tcgtcttcca cgcgacggct cgcggcgact ccgtcgacct
      241 ctcggccacg ggaattaccg cagacgacga gacgcggggt ctgctggaag atgctctgaa
      301 aggcgtcgtt gcgatgtggc gcctcgcgat tcacgagaag tcgcctgagc tggtgccgat
      361 cctccgcaag ttcgagatcg agagcgacca cgaaggcgtc acgatccacg gcacgttgcc
      421 cgggtccttc ctgcgcaaga tggcggagga gcggcacgcc cgccgggacg actagagggg
      481 gtcaggtctc cacttgcgac ttcgcttcgc gaagatggca agagcggcca cggcgcattc
      541 gccacgcggg ctgttcgtga aagacctgac cctaccaggg cacgtcccgt ccgtgccggt
      601 cgagaaatcg gccggcgtcg gacgggcgga agccgtcgat caccttcagc atctctgcga
      661 cgctttcgtc cggcgtcagc ggggcgttcg ggccgcccat ggccgtgcgc acccatcccg
      721 ggctcaacac ggcgcaggtg aatcgctcct tcgcgagctc ctccgcgagc gtgcggacga
      781 acatgttgag ggccaccttc gacatccggt aggcgtacca gccgccgtcg gtgttctggg
      841 acaggctccc gagtccggaa gtgatcgcga cgaccttgcg caaccggccc gcgaagaggt
      901 tcggaaggag cgcgcgggtc acgcgttcgg gaccgactgc gttgacgtcc aaagcccgga
      961 gcaccgcatc gggctcgagt tcttcgatcc tcgatccctc ctcgcccacc gcggcgttgt
     1021 ggacgaggac gtcgaccggc acgccctcca ggcgtcgcac gaagtccgcg acgctcgaat
     1081 ctttcgagac gtcgagcttc tcgacccggg cgcccgccgc gcggagctcg ttcgcgccgg
     1141 ccggctggcg cgccgtcccg acgacagtct caccgcgctt cgagagggcc cgcgcgaacg
     1201 cgaggccgat cccccggttg gcgcccgtga cgacccaggt tcccatgaca gccgttcatc
     1261 ataaggaggc gggtccaggc ccgccgggcc tgtccccgcg acgggctaag atcgcagtgg
     1321 tgtcacaggc cgtctctcaa cttccaccgg aaatcgccgc tctcgtcgat cagaagcgcc
     1381 tcgaggagct cgaggacgtc tggacccggc gcatggagca ggatccgtca gacctgcagt
     1441 tctttttccg gatcgccgcc gccgtcaaga agaaggcggg agcggaaggg gcagagcagg
     1501 cgctttcgtg gctgcggttt ctcgccgact accagtcgga gcggggggat cccgaggcgc
     1561 gaatcgaggt gctcgccgaa atcgttcgga tgaatcccct ggacctgtcg gcgcgagccg
     1621 atctcgaacg cgcgctcgcc gagcgctacc gggaccaccc ggtgctcgcc gcggtgatgg
     1681 cgcagaaccc gatcgcgaac gcctccgatc cgtccgagac cgcgcgccgc gtcgcgcgtt
     1741 ggctgaggtt cttgccggga ggggtgtact acctcgccgg acggggcgcc ggccggatcg
     1801 cggaaatgaa cccggctctc gacgtgatcc ggctcgaagt gggcggcgcg cgggtgccgc
     1861 tgtcgctcgt ctccgccgag aagaacctcg agccgcttcc ggccggccac ttcctccgcg
     1921 aaaaggtcga gaacctggac ggcctgcgag acctcgtctc gagagagccc gcggaggcgg
     1981 tgcgccgcct tctttcgagc ttcgggcgcc cgctctccgt gggagaggtc cgggaacatt
     2041 tttcgggcgt cgtcgacgac gcgcgctgga gctcgttctg gacgtcggcc cgccgccatc
     2101 cgcagctcct cgtcgccggg gcggcgaaga gcgctctcgt ctcctggagc gagagcgccg
     2161 acgcggcgga cgagacggtg cgccgcgaat tcgagtccgc cgagccgcag aagaagctcg
     2221 agatcgcgcg ccggcacgcg aagcgctcgc gtgagctcgc tcggctcttc gcccgttctc
     2281 tcgcctcgga agccggcagc gcggccgatc gcggcgacgc cgcgctcgcc tgggagcttt
     2341 cgcaggcggc cgcgcggctc gctccgggag agcccgaggc atttccggcg gaatcgctgc
     2401 tcgcgtcctc gaacgtcgcc gcggtcctcg gatccatccg ggatcacgtc gcccgcgaaa
     2461 aggcgctcga acaggttcgc gcgaggcgcg acgactggcc ggcaatcttc gcggcgcggt
     2521 tccaggaaga ggaagacgcg cgcgttctct ccgcgctctt cgaggcgctc ggagaagacc
     2581 gatcccgccg ggacgaactc gtgcgccgcg tcatgcgctc tccgaggatc gccccgcgcg
     2641 cgttcgtctg gctctgcgag cggtcggggg ccgatgccag cccggacctg tccgcgtccg
     2701 cgctctttca ctcgcttctc gacgcgcttc ggcaggacga gttcggctcg gtgcgcgccc
     2761 gcgtgaaggc cttcttcgag cccggagcac tcgcggtcga cctcgtcaag cgcgtcggaa
     2821 cggaggaaga ggcgcgcgac ttcgtgaacg cgctcgcccg ggccggcggc ctcgaagagc
     2881 accgtcgcgg caccgtgagg gaggcgctcc tgatgaggtt ccccaccctg cgggctcccg
     2941 cgatgaatta cctctacgcg acgccggagg cgatcgagag gcggcgcaac gagctcgcgc
     3001 aactgaagca ggtggagctg ccggccaacg ccgagcagat gcgcgcggcg aaggagcacg
     3061 gagacctctc cgagaacttc gagtaccacg cggcgcgtca gaagcacgag tacctctcgg
     3121 cccggatcgc gaagctcgcc gacgagctct cccgaacgcg cgcgctcgat tcgacccgca
     3181 tcgacgcttc cgaggtgcgg gtgggcacgc gcgtcgttct ccgggagaag gagagcggcc
     3241 gcgaacgcat cgcgacggtt ctcggaccgt gggactcgca gccggagctc cagatctact
     3301 cgtacgagtc cgaattcgcg accgcgcttc tcgggaaacg gccgggagag acggtcaccc
     3361 ttccggacgg cacgctcgaa gtcgtttcga tcagcccctg gcgatagaat tcccgccgaa
     3421 aaatccgatc gaggaggtcg tcatgaagag cggagtcgct cgggttctcc cctttctcgt
     3481 tttggccctc tcctcagtct catcctcggc gattgctcag gcgccggcgg cggcgtcgaa
     3541 ggcgtcattc ggctcggaga tctcgaagga actggcggac gtcgagaaga agctcgtgac
     3601 cctcgcggag gcgatgccgg ccgagaaatt cggctggcgt cccgcggaag gagtccgctc
     3661 cgtgagcgag gtctacgttc acgtcgccgg cggcaactac atgctgccca cgatgttcgg
     3721 cttcaagcgg cccgagggga tggtcggccg cgacatggag aa
//
LOCUS       DASWAV010000275         2789 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U2665
            k141_694928, whole genome shotgun sequence.
ACCESSION   DASWAV010000275 DASWAV010000000
VERSION     DASWAV010000275.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960316
            Sequence Read Archive: SRR10260007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2789)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2789)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:47:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,929
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2789
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_694928"
                     /isolate="SMAG_U2665"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.36444 N 132.31889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..664)
                     /locus_tag="VF958_05665"
     CDS             complement(<1..664)
                     /locus_tag="VF958_05665"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX9148634.1"
                     /translation="MRSVLLVDKGSLFRDVARSVVRRTNCRILIARTGAEALSVARRE
                     KPDLVFLDAKMEGMTGVDVCRVLKADKQFSHRPIIVVGGGEKERTEGTRAGADGFLTG
                     DFAEEEFFETLRRHLNVFARDAARSAAGWSITFWRDGAQHQGKIRDLSRGGLFVRTGV
                     EQPIGARLEISFETPVESANRSIVGEAIVVRLGRAPDPGLGCRFFQLSSTSRTNLEDC
                     LKM"
     gene            complement(705..1424)
                     /locus_tag="VF958_05670"
     CDS             complement(705..1424)
                     /locus_tag="VF958_05670"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX9148635.1"
                     /translation="MTSQSLEGKVALITGASSGLGRGFALALARAGAKVFLVARSEEK
                     LRDAVEEIAAAGGEAAYHVADVRITPTLFDLVDVLLARFKRLDVLVNNAGLGYRAPLL
                     ELKRSEILEMLETDLHAAIFLCQAALTALLKSAPSDIVNISSIAGLEGFAEGTAYCAA
                     KSGLVGFTRALAQELKPANVRATAICAGSIDTPFFERFRPTVEPAKRLIVEDAVRALM
                     YVVTSPPNVLHGEIVIRPRVV"
     gene            1457..2527
                     /locus_tag="VF958_05675"
     CDS             1457..2527
                     /locus_tag="VF958_05675"
                     /inference="COORDINATES: protein motif:HMM:NF016214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HEX9148636.1"
                     /translation="MDTVSHGIAGAVFSRALTDRPAARAALLLGLVGAMLPDVDMFFL
                     NTRMAYLHGHRGWTHTFFLLPVFAIALGVLAKLIYRYARHARLSILVLFAAVGIATHI
                     VFDWITSFGIMFLIPFTRRRFALDWVFILDPYFTGIVSVWLLATTIFRPKGRFLSSLG
                     VLMLGAYVAVCAILHAKALSVWKGMDSPPSGAKVGVLPQFLSPFRWLGVTERPDDIHV
                     AFFDIGPFARGNPSPHPPTRLSEIFSSLSDYYPPPDRARIQRYDKPRPSPALAAAEKL
                     PDVRTYLEFARFPLATVRAESDGATSISFQDLRFLPWFSGPWERERGGGIRFRRQPFV
                     YRVRLDSTGRPLEGGFVGTASR"
     gene            complement(2477..>2789)
                     /locus_tag="VF958_05680"
     CDS             complement(2477..>2789)
                     /locus_tag="VF958_05680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9148637.1"
                     /translation="RPGRLLGVLLLYVICGAALYAIWIVFDPSAGGEARFALVPLILT
                     QQVFVFLRSLIKVGYYAGVSEALTRVPSPEYSYVAPPAVDLPLSSGRGADEAPLERPP
                     G"
BASE COUNT          441 a          961 c          895 g          492 t
ORIGIN      
        1 gcatcttcag gcaatcctcc aggttcgtac gcgaggtcga ggagagctgg aagaaacggc
       61 agccgagtcc cggatccggc gcgcgcccga gccggacgac gattgcctcg ccgacgatgg
      121 aacggtttgc gctctccacc ggcgtctcga aggagatctc caggcgcgcg ccgattggtt
      181 gctccacccc ggttctcacg aaaagaccgc cgcgcgagag gtctcggatc ttcccctggt
      241 gctgggctcc atcccgccag aacgtaattg accatccggc ggccgaccgc gctgcgtcgc
      301 gcgcaaagac gttcaggtga cggcgcagcg tctcgaagaa ctcctcctcc gcgaaatccc
      361 ccgtcaggaa accgtccgcg ccggcccgcg tcccctcggt gcgctccttt tccccgccgc
      421 cgacgacgat gatcgggcgg tgcgagaact gtttgtccgc cttcagcacg cggcagacgt
      481 ccactcccgt catcccttcc atcttcgcgt cgagaaagac gaggtccggc ttctcgcggc
      541 gcgcgaccga gagcgcctcc gcgcccgtcc tggcgatcag gatgcggcag ttcgtccgcc
      601 ggacgaccga gcgtgcgacg tcgcggaaaa ggctcccttt gtccacgagg aggacggaac
      661 gcatgacgac cccgattata cggacgcccg cgggaactgc gcccttagac cactctcggt
      721 cggatcacga tctcgccgtg gaggacgttc ggcggcgagg tgacgacgta catcagggcc
      781 cgaacagcgt cctcgacgat gagccgcttc gccggctcga cggtcgggcg gaaccgctcg
      841 aagaacggcg tgtcgatcga tccggcgcaa atcgcggtgg cgcgcacgtt cgcgggcttg
      901 agctcctgcg cgagagcgcg ggtgaacccg acgagcccgg atttcgccgc gcagtaggcg
      961 gtcccctcgg caaacccttc gagccccgcg atcgacgaga tgttcacgat gtcggaaggc
     1021 gcgctcttga gcagtgcggt cagcgccgcc tggcagagga agatcgccgc gtggaggtcg
     1081 gtttcgagca tctcgaggat ctccgagcgc ttcagctcga ggagcggcgc gcggtagccc
     1141 agccccgcgt tgttcacgag gacgtccagc cgcttgaagc gcgccaggag gacgtcgaca
     1201 agatcgaaga gcgtgggcgt gatccgtacg tccgcgacgt ggtacgcggc ttcgccgccc
     1261 gcggcggcga tttcctcgac ggcgtcgcgg agcttctctt cgctcctcgc cacgaggaac
     1321 actttcgcgc cggcgcgggc gagcgcgagg gcaaatccgc gcccgaggcc ggacgaggcg
     1381 ccggtgatga gcgccacctt tccttcgagg gactgactgg tcacggctga ttggacagct
     1441 gatagcttct caccgaatgg acacggtcag ccatggcatc gccggcgccg tcttctcgag
     1501 ggcgctgacc gaccgtccgg ccgcgcgagc ggcgctcctg ctggggctcg tgggcgcgat
     1561 gctgcccgat gtggacatgt tttttctgaa cacccggatg gcctaccttc atggccaccg
     1621 cggttggacc catacctttt tccttctccc cgtcttcgcg atcgcactcg gggtgctcgc
     1681 gaagctcatc tatcgttatg cgcgccacgc gcgcctgtcg atcctcgttc tgttcgccgc
     1741 ggtcggcatc gcgacccaca tcgtcttcga ctggatcacg tctttcggga tcatgtttct
     1801 gatccccttc acgcgacggc gcttcgctct cgactgggtg ttcatcctcg acccgtactt
     1861 caccggcatc gtctcggtct ggctcctggc gaccacgatc ttccggccga aggggcgctt
     1921 tctctcttcc ctcggggtcc tcatgctcgg cgcctacgtg gcggtgtgcg cgattcttca
     1981 cgcgaaggcg ctctcggttt ggaaaggcat ggattcgccc ccttccggtg cgaaggtcgg
     2041 cgtgctgccg cagtttctct caccgtttcg gtggctcggc gtcacggagc ggccagatga
     2101 catccacgtc gccttcttcg acatcgggcc cttcgcgcgg gggaacccgt cgcctcaccc
     2161 gccgacgcgt ctttccgaga tcttctcgag cctctccgat tactacccgc cacccgaccg
     2221 cgctcggatc cagcgctacg acaagccgcg cccctcaccg gcgctcgcgg ctgcggagaa
     2281 actgccggac gtccggactt atctcgagtt cgcgcgcttc ccgctcgcca ccgtgagagc
     2341 agaatccgac ggcgcaacgt cgatctcttt ccaggacctg cgctttctcc cctggttcag
     2401 cggtccctgg gagcgcgagc gggggggcgg gatccgcttc cgtcgccagc cgttcgtcta
     2461 ccgcgtgcga ctcgattcaa ccgggcggcc gctcgagggg ggcttcgtcg gcaccgcgtc
     2521 ccgatgacag gggaagatcg accgccggcg gagcgacgta cgaatactcc ggcgacggga
     2581 cgcgcgtgag cgcctcggag acgcccgcgt agtaaccgac cttgatgagc gagcggagga
     2641 agacgaagac ctgctgagtc aggatgagag gcacgagcgc gaaccgggct tcgccccctg
     2701 cggagggatc aaaaacgatc cagatcgcgt aaagcgccgc gccgcagatc acgtacagca
     2761 agaggactcc cagaaggcgg ccgggccgt
//
LOCUS       DASWAV010000276         2700 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U2665
            k141_15935, whole genome shotgun sequence.
ACCESSION   DASWAV010000276 DASWAV010000000
VERSION     DASWAV010000276.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960316
            Sequence Read Archive: SRR10260007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2700)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2700)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:47:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,929
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2700
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_15935"
                     /isolate="SMAG_U2665"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.36444 N 132.31889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..732)
                     /locus_tag="VF958_05685"
     CDS             complement(<1..732)
                     /locus_tag="VF958_05685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9148638.1"
                     /translation="MTRFDVARRTALVQDLLAQARGRPTDLLPFDAVREKFRLRRLVD
                     RGIQEVPLERIAGTVQREREFNRAFLPRDESLRDRWQEIEGLAEGAKGFPPVELYRVG
                     ETDFVVDGHHRVSVARSLGASTIEAHVKEFETAVPLDPTSSIEDVVLKEGLSDFLETT
                     GLSPERPDEFRTTEANGYERLIDHINVHRHFRGVNERREIPWPEAVRSWRDTVYRPMI
                     ERIETSGVLEEFPGRTPTDLYLFTMD"
     gene            769..2421
                     /locus_tag="VF958_05690"
     CDS             769..2421
                     /locus_tag="VF958_05690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013374605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HEX9148639.1"
                     /translation="MRPLHRLLVVLTLALAFATCASQSRQRPPTRVVAPRGASQWSHD
                     WAKGAVFYEVFVRSFSDSDGDGKGDLNGLVSRLDYLNDGDATTTSDLGVDAVWLMPVF
                     RSPSYHGYDTTDYETINPDYGSNADFDRFLAEAHRRGIRVILDFVMNHTGSDHPWFID
                     SSSGASSAKRDWYVWSPVDLGWMQPWGNNPTWHPKNGAFYYGIFWSGMPDLNFRTPAV
                     RTEIERLARLWLDRGVDGFRLDATRHLIETGPGPGQSDTSETHVFLKEFAASIRGSHP
                     SATLVGENWTETPTIATYYGSTATVAGGDELPMNFDFPLAERIVRGVNAGDGGAIAEK
                     IAEIRSLYPAGSLDAPFLTNHDQVRVATQLSRSLPKMRNAAAILLTLPGAPFLYYGEE
                     VGLQNGTTGNDEAKRTPMPWDGTAGGGFTSGSPWFPFAPGHETENVAAQTGDRASLLS
                     RYRDLIRLRHTSTALARGGIDLLTPSGFSPTLAYLRVNGAERVLVVHNLTDSFVNGGP
                     YAVTAVGSERLFTDEGVGDPSGPSGEWRVTLAPRASAVFRLR"
     gene            complement(2428..>2700)
                     /locus_tag="VF958_05695"
     CDS             complement(2428..>2700)
                     /locus_tag="VF958_05695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9148640.1"
                     /translation="TSALAHLVVLLTKHLGASEAARKLDVLDPLRRFESVFPSLGRRL
                     EAALGLETPAAARALLDIALEELPGRIPASAVAAIRPRLDPGSAVD"
BASE COUNT          426 a          919 c          901 g          454 t
ORIGIN      
        1 gtccatggtg aagaggtaaa gatcggtcgg cgtgcggccc gggaattcct cgaggacgcc
       61 gctcgtctcg atgcgttcga tcatcggccg gtagacggtg tcgcgccagg atcgcaccgc
      121 ctcgggccac gggatctcac gcctctcgtt gaccccccgg aagtgccggt ggacgttgat
      181 gtggtcgatc agtcgctcgt atccgttcgc ctccgtcgtg cggaactcgt cgggtctttc
      241 gggcgagagg ccggtcgtct cgaggaagtc ggagagccct tccttcagaa cgacgtcctc
      301 gatcgacgag gtcgggtcga gaggcaccgc cgtctcgaac tccttcacgt gggcttcgat
      361 cgtcgatgcg ccgagtgaac gcgcgaccga cacgcggtgg tgaccgtcga cgacgaagtc
      421 ggtttcaccc acccggtaga gctcgaccgg cgggaaccct ttcgctccct cggcgaggcc
      481 ttcgatctct tgccaccggt ctctcaggga ctcgtcgcgc gggagaaacg cacgattgaa
      541 ctcgcgctct cgctgcacgg tcccggcgat ccgttcgagc gggacttcct ggatgccgcg
      601 gtcgacgagt cttcggaggc ggaacttctc gcgcacggcg tcgaagggca gaaggtcagt
      661 cgggcgcccg cgggcctgcg cgaggagatc ctggaccagc gcggttcgac gcgcgacgtc
      721 gaaccgggtc acgctcgcga ctccggctgg ttagaatccg gtgtgcccat gcgtccgctt
      781 caccggctcc tcgtcgttct gaccctcgcc ctcgcgttcg cgacctgcgc gtcccagagc
      841 cggcagcgtc ccccgacgcg cgtcgtggcg cctcggggag cctcgcagtg gagccacgat
      901 tgggcgaagg gcgccgtttt ctacgaggtc ttcgtccgga gcttctccga ttccgacggg
      961 gacgggaagg gagacttgaa cgggctggtc tcgcggctcg actacctgaa cgacggcgac
     1021 gcgaccacga cctcggacct cggcgtggac gccgtctggc tgatgcccgt cttccgttcg
     1081 ccgagctatc acggctacga caccacggac tacgagacga tcaaccccga ctacggctcg
     1141 aacgccgact tcgatcgctt cctggccgaa gcgcaccggc gcggcatccg ggtcattctc
     1201 gatttcgtga tgaaccacac cgggtccgac cacccgtggt tcatcgattc gagctccggc
     1261 gcgtcctcgg cgaagaggga ctggtacgtc tggagcccgg tcgacctcgg ctggatgcag
     1321 ccgtggggca acaacccgac ctggcacccg aagaacggcg cgttctacta cgggatcttc
     1381 tggagcggca tgccggacct gaacttccgc actcccgcgg tccgaaccga gatcgagcgc
     1441 ctcgcccgcc tctggctcga tcgaggcgtg gacgggtttc gcctggacgc gacgcgccat
     1501 ctcatcgaga cggggcccgg acccgggcag tcggacacgt cggagaccca tgtgttcctg
     1561 aaggagttcg cagcgtccat ccgcggctcg cacccgtccg cgacgctggt gggggagaac
     1621 tggacggaaa ccccgaccat cgccacgtac tacggatcga cggcgaccgt cgcagggggc
     1681 gacgagctcc ccatgaactt cgattttccc ctggcggagc ggatcgtccg tggcgtcaac
     1741 gcgggggacg ggggggcaat cgcggagaag attgccgaga tccggtcgct ctatccggcc
     1801 ggctccctgg acgctccctt tctcaccaac cacgaccagg ttcgcgtggc gacgcagctc
     1861 tcgagaagcc tgccgaagat gaggaacgcc gcggcgatcc tcctgacgct gcccggtgcg
     1921 cccttcctct actacggcga ggaggtcgga ctccagaacg ggacgaccgg gaacgacgaa
     1981 gcgaagcgaa cccccatgcc ctgggacggc acggcgggag gaggtttcac gtccggatcc
     2041 ccgtggttcc cgttcgcgcc cggccacgag acggagaacg tcgcggcgca gaccggtgat
     2101 cgggcatctc ttctctcgcg atatcgggac ctgatccgcc tccggcatac gtccaccgcg
     2161 ctcgcgaggg gaggcatcga ccttctcact ccttcgggat tctccccgac gctcgcttac
     2221 ctgcgggtga acggggccga gcgcgttctc gtcgttcaca atctcaccga ttcgttcgtc
     2281 aacggaggtc cgtacgcggt gaccgcggtc ggctccgagc gcctcttcac cgacgagggc
     2341 gtcggcgatc cttccgggcc cagcggggaa tggcgcgtga cgctcgcccc gcgtgcatcg
     2401 gccgtcttcc ggctgcggta gtccgcacta atctaccgcc gatcccggat cgagccgggg
     2461 ccggatcgcg gcgacggctg acgcgggaat ccgcccgggg agctcttcga gggcgatgtc
     2521 gaggagcgcg cgcgccgccg cgggagtttc cagcccgaga gctgcttcga gccggcgccc
     2581 gagcgacggg aacaccgatt cgaaccgccg caacggatcg aggacgtcga gcttcctcgc
     2641 cgcttcggac gccccgagat gtttggtgag gagcacgacc agatgggcga gcgcactcgt
//
LOCUS       DASWAV010000277         1655 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U2665
            k141_175527, whole genome shotgun sequence.
ACCESSION   DASWAV010000277 DASWAV010000000
VERSION     DASWAV010000277.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960316
            Sequence Read Archive: SRR10260007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 1655)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1655)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:47:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,929
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1655
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_175527"
                     /isolate="SMAG_U2665"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.36444 N 132.31889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1655)
                     /locus_tag="VF958_05700"
     CDS             complement(<1..>1655)
                     /locus_tag="VF958_05700"
                     /inference="COORDINATES: protein motif:HMM:NF019335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEX9148641.1"
                     /translation="IGIFLIAGAGVALGQGTTATLAGTVTSGGTALPGVLVTVSSPAL
                     QGTRSAATGSSGSYSIAGLPPGDYTVTFELAGMQTKTQKARLILAQTTRADEELSVSG
                     VKEEVSVKGEAEAVLETTQIATNFTSEQIARLPVARTIVNTILLAPGVSNTGVNNQVT
                     ISGAPSYDNTFLVNGVVVNENLRGQPHNLFIEDAIQETTVLSGGVSAEYGRFTGGVVS
                     TLTKSGGNTFSGSFRDSLSNPKWVEKTPWPAEADHVDKTDSVYEATLGGRILKDHLWF
                     FGAGRLAKRSLQRFTAITNLPFTNGFDEKRWEGKLTGQIATGHNLVVSYLNIKNDETN
                     NFFGNVLDYDSLVPTRSLPNTLLAVNYNGVLTKNLIVEAQYANKKFTFENSGGLLTDR
                     IGGTLLVDTNGRRFHAPTFCGVCSFEERNNDSWLGKATYYLDTPRLGSHSIVFGAENF
                     AEKRLANNHQEGSDYRISSVTSIIAGTTVYPRFGSASVIQYQPILSNSEGTDFQTFSA
                     FVNDKWDFNRHFSFNVGFRYDKNNGKDADGNTVSDDSAFSPRLG"
BASE COUNT          258 a          495 c          567 g          335 t
ORIGIN      
        1 ccgagccgtg gcgagaacgc gctgtcgtcg gagaccgtgt ttccgtcggc gtccttcccg
       61 ttgttcttgt cgtagcggaa tccgacgttg aagctgaagt ggcggttgaa atcccacttg
      121 tcgttgacga acgcggagaa cgtctggaag tccgtcccct cggagttcga gaggatcggc
      181 tgatactgga tgacgctcgc gctgccgaag cgcgggtaga ccgtcgtccc ggcgatgatc
      241 gaagtgacgc tcgagatccg gtagtcgctc ccctcctggt ggttgttggc cagtcgcttc
      301 tcggcgaagt tctccgcgcc gaacacgatc gagtggctcc cgaggcgcgg cgtgtcgagg
      361 tagtaagtcg cctttccgag ccaggagtcg ttgttgcgct cttcgaagct gcagacgccg
      421 cagaacgtcg gcgcgtggaa gcggcgcccg ttcgtgtcca cgagaagcgt gccgccgatg
      481 cggtcggtga gaaggccgcc gctgttctcg aacgtgaact tcttgttcgc gtactgcgcc
      541 tcgacgatga ggttcttcgt caggacgccg ttgtagttca cggcgagaag agtgttcgga
      601 agagaccgcg tcggcacgag gctgtcgtag tcgaggacgt ttccgaagaa gttgttggtc
      661 tcgtcattct tgatgttcag gtacgacacc acgaggttgt gcccggtcgc gatctggccg
      721 gtgagcttgc cttcccagcg tttctcgtcg aaaccgttcg tgaagggcag gttcgtgatc
      781 gccgtgaaac gctgcaacga gcgcttggcg aggcggccgg cgccgaagaa ccagaggtgg
      841 tccttcagga tcctcccgcc gagcgttgcc tcgtagacgg aatcggtctt gtcgacgtgg
      901 tccgcttcgg cgggccaggg ggtcttctcg acccacttcg ggttggagag gctgtcgcgg
      961 aaggagccgc tgaaggtgtt cccgccggac ttcgtcagcg tcgacacgac gccgccggtg
     1021 aagcgaccgt attcggccga tacgccgccc gagagaaccg tcgtttcctg gatcgcatcc
     1081 tcgatgaaga ggttgtgcgg ctggccgcgc aggttctcgt tgacgacgac tccgttgacg
     1141 agaaacgtgt tgtcgtagga gggcgctccg gagatcgtga cctggttgtt gacgcccgtg
     1201 ttgctgacgc cgggggcgag caggatcgtg ttgacgatcg tccgggcgac cggaagccgt
     1261 gcgatctgct ccgacgtgaa gttcgtcgcg atctgggtcg tctcgaggac ggcctcggcc
     1321 tcccccttga cgctcacttc ttccttgacg cccgacaccg agagctcctc gtccgcgcgg
     1381 gtggtctgcg cgaggatgag tcgggccttc tgagttttcg tctgcatgcc cgcaagctcg
     1441 aaggtgaccg tgtagtcgcc gggaggcagc cccgcgatcg agtaactgcc gctcgatccg
     1501 gtcgcggccg acctggtccc ctgaagggcg ggcgacgaga cggtcacgag caccccgggc
     1561 agcgcggttc ctccggacgt caccgtcccc gcgagcgtgg cggtcgtgcc ctgcccgagc
     1621 gcgacaccgg cgcccgcgat caaaaagatg ccgat
//
LOCUS       DASWAV010000278         1663 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U2665
            k141_137004, whole genome shotgun sequence.
ACCESSION   DASWAV010000278 DASWAV010000000
VERSION     DASWAV010000278.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960316
            Sequence Read Archive: SRR10260007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 1663)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1663)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:47:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,929
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1663
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_137004"
                     /isolate="SMAG_U2665"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.36444 N 132.31889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..381
                     /locus_tag="VF958_05705"
     CDS             <1..381
                     /locus_tag="VF958_05705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9148642.1"
                     /translation="TIRSAAPSGSLLFADLRMGHVLYYYLGRNEPNPANRSREGFREN
                     VVGGYRLVRSPVWNFNAKRFGSEWRRLIDVYELAAGQTVWLVRVGSEYNPGAVLLQIY
                     SVTTLLREFRFGEISVFELRVPEQ"
     gene            595..1641
                     /gene="purM"
                     /locus_tag="VF958_05710"
     CDS             595..1641
                     /gene="purM"
                     /locus_tag="VF958_05710"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007393010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004641 -
                     phosphoribosylformylglycinamidine cyclo-ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HEX9148643.1"
                     /translation="MKSSRYAAAGVDIDAKAAALERAKAAIRSTFTPSVLGDVGGFGG
                     LFRPDLTGYRDPVLVASTDGVGTKLRVAFETGRHDTCGADLVNHCVNDVLVQGAKSLF
                     FLDYVAAGKIEPSVLEKVIEGIARGCRENGIALLGGETAEMPGFYVPGEYDVAGTIVG
                     IVEREKILDGSRVCAGDIALGLPSAGLHTNGYSLARHVLFARLKKTPQDRLPELGGST
                     IGDVLLAPHLSYWKALEPLLARDLVHGMAHITGGGFFDNIPRVVPKDLEVVIKGGSWP
                     VPPIFEVIERAGEVSFEEMHRVFNMGVGMVVFAAPDELGEISRTWTNMGQPWFAIGNV
                     RAGDRRVVVELPPR"
BASE COUNT          267 a          536 c          538 g          322 t
ORIGIN      
        1 accatccgct cggccgcgcc ttccgggagt cttcttttcg ccgacctgag gatggggcat
       61 gtcctctact attatctcgg tcgcaacgag cccaatcctg cgaaccggag ccgagaaggg
      121 ttccgcgaga acgttgttgg cggttaccgc ctcgtgcgct cacccgtatg gaatttcaac
      181 gccaagagat tcggctccga gtggcgccgc ttgatcgacg tatatgagct tgccgccgga
      241 cagaccgtct ggctggttcg agtgggatcg gagtacaacc ccggtgctgt gcttcttcaa
      301 atctatagcg tcaccactct gcttcgcgaa ttccggttcg gggagatttc ggtcttcgaa
      361 ctgagagtcc cggaacagtg accacccgca gcgcagctgg atagcccgac tctgccggtt
      421 ttttcgttcc cggatcggaa gccctcgacg ggaggaaggg cagacgctcc gcttctgaaa
      481 gagtcgcgcg cggctctcgt cgctggcggg cattttcacc cggttggcca ctcccggaac
      541 ggggattcct gaatcccgcc cgatcgtcgc aggcaccgct ataatttctg cttcttgaaa
      601 tcctcccgtt acgcggccgc cggtgtcgac atcgacgcga aggcagcggc gctcgagcgg
      661 gcgaaagccg ccatccgctc cacgttcacg cccagcgttc tcggcgacgt tggtgggttc
      721 ggcggtctct ttcgtcccga cctcacgggc tatcgcgacc ccgtcctcgt cgcctccacg
      781 gacggcgtcg gcacgaagct cagggtcgct ttcgagacgg gtcggcacga cacctgcgga
      841 gcggatctcg tcaaccactg cgtcaacgac gttctcgtgc agggggcgaa gtcgctcttc
      901 ttcctcgact acgtcgcggc ggggaagatc gaaccgtcgg tgctggagaa ggtcatcgag
      961 ggcatcgcgc gcggctgccg tgaaaacggc atcgcgctgc tcgggggcga gaccgccgag
     1021 atgccgggct tctatgtgcc cggcgagtac gacgtcgcgg gcacgatcgt gggcatcgtg
     1081 gagcgggaga agatcctcga cggttcgcga gtgtgcgccg gggacatcgc gctcgggctc
     1141 ccgtccgcag gcctccacac caacggttac tctctcgcac gccacgtcct gttcgcacga
     1201 ctgaagaaga cgccgcagga tcggctgccg gagctcgggg gctcgacgat cggggacgtg
     1261 cttctcgccc cgcacctctc ctactggaaa gcgctcgagc cgctgctcgc tcgcgacctc
     1321 gttcacggca tggctcacat cacgggtggc ggattcttcg acaacatccc gcgtgtcgtt
     1381 ccgaaagatc tcgaggtcgt catcaagggc ggctcgtggc cggtcccccc gatcttcgaa
     1441 gtgatcgagc gcgcgggcga ggtctccttc gaggagatgc accgcgtctt caacatgggt
     1501 gtggggatgg tcgtcttcgc ggcgcccgac gaactcggcg agatctcgcg aacctggacg
     1561 aatatggggc agccgtggtt cgcgatcgga aacgtgcgcg caggggatcg ccgggtcgtc
     1621 gtcgagctgc cgccgcggta atgcgccgcc gccgtatcgc ggt
//
LOCUS       DASWAV010000279         3025 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U2665
            k141_495373, whole genome shotgun sequence.
ACCESSION   DASWAV010000279 DASWAV010000000
VERSION     DASWAV010000279.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960316
            Sequence Read Archive: SRR10260007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 3025)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3025)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:47:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,929
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3025
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_495373"
                     /isolate="SMAG_U2665"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.36444 N 132.31889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..509)
                     /locus_tag="VF958_05715"
     CDS             complement(<1..509)
                     /locus_tag="VF958_05715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011551258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HEX9148644.1"
                     /translation="MDNLCHTLVGAALGEAGLARRKPLATATLLIGANLPDVDAFTYA
                     FSDGPTSLAFRRGWTHGVLAMAVLPVLLAAAMVAFDQLVRRRRRKEKIPARFRALLVP
                     AFASVLSHPLLDFLNTYGVRFLYPFSKRWFYGDTLFIVDPWVWIALAVGIASSRVFRR
                     RRPAQAEAPA"
     gene            575..1459
                     /locus_tag="VF958_05720"
     CDS             575..1459
                     /locus_tag="VF958_05720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015179728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HEX9148645.1"
                     /translation="MKIRSVKTVVPALATLEGAGFLVHRPFPSPALDFFDPFLLLDEM
                     GPSELGPGEAKGAPDHPHRGFETVTYLLSGAMQHKDSQGNQGKLTPGDVQWMTAGSGV
                     VHSEMPEPSFQKTGGRMHGFQLWVNLPARDKMTKPRYQEIPGAGIPVAKSEDGKVAVR
                     VIAGEALGKRAVIETRTPIFYLDCTLQPGASLTQPAPAEYNTFAFVYAGRGSFGSNAS
                     PTRVHEAVIFEDDGDTVRLEAAADAPLQALLIGGVPLNEPVARYGPFVMNTRDEIVQA
                     FADYQSGKLGEISGPGLH"
     gene            1591..2022
                     /locus_tag="VF958_05725"
     CDS             1591..2022
                     /locus_tag="VF958_05725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9148646.1"
                     /translation="MSRRIGIAVGLSVAVFLPAVADAESSRPVQPSTFLTLGGAYTVE
                     AGGCERHSRNFRVSSRRPIDISKVEKGQIVSGVHFKERERAGRAGWRNVSVSSDGRAV
                     DFELFAEGAGHIQSVPSEGRQCVEAAPARVVVDVEAWVVDP"
     gene            2139..2786
                     /locus_tag="VF958_05730"
     CDS             2139..2786
                     /locus_tag="VF958_05730"
                     /inference="COORDINATES: protein motif:HMM:NF015325.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HEX9148647.1"
                     /translation="MPPAESPLKVLVLNASLKRSPEISNTGEVAQLVLDFMKPYGIQG
                     EMIRLADHMIPTGLGFREGPDDDWPKIVGKIKASEALIFATPIWWGGRSSLMQRVIER
                     PDSLDEEYIASGRSALYNKVAGVVITGSEDGALSTLGSIMMVLTFMGFTLPPECAAYW
                     VGEVGNPPSQDRDRRLRNKATPHMAKNMARNLVYYARLLNQHPLVTERGEAAAEV"
     gene            complement(2790..>3025)
                     /locus_tag="VF958_05735"
     CDS             complement(2790..>3025)
                     /locus_tag="VF958_05735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9148648.1"
                     /translation="SEVDMPDSRVSDLLEAQVDQHTPHQKKAFLEAFNNAYEEYGHDE
                     SRAFAVAHSAAKKAGGGPAREHGASGRTERAES"
BASE COUNT          533 a          985 c         1016 g          491 t
ORIGIN      
        1 gccggggctt ccgcctgcgc cggacggcgc cttcggaaaa cgcgcgaaga ggcgatcccg
       61 accgcgaggg cgatccacac ccacgggtcc acgatgaaga gggtgtcccc gtagaaccaa
      121 cgtttcgaaa agggatagag gaaccgcacc ccgtacgtgt tcaggaagtc gagcagcggg
      181 tgggacagga cggaggcgaa cgcggggacg agcaacgccc ggaaccgcgc cggaatcttt
      241 tccttccgcc gacggcgccg gaccagctgg tcgaacgcga ccatcgccgc ggcgagcaga
      301 acgggaagga ccgccatcgc gaggaccccg tgcgtccagc cgcgccggaa cgcgagggac
      361 gtcggaccgt ccgagaacgc gtaagtgaag gcatcgacgt ccggcaggtt cgctccgatc
      421 aggagcgtcg cggtcgcgag cggcttgcgc cgtgcgagcc cggcttcccc gagcgccgcg
      481 ccgacgagtg tgtggcagag gttgtccacg cggggtttat acgcgaggaa tccgtttcgc
      541 gcggctggag tatttcttgc tcgggagatc aaccatgaaa atccgatccg tcaaaaccgt
      601 cgttcccgcc cttgccacgc tcgagggcgc gggcttcctc gtccaccggc cgttcccctc
      661 tcccgccctc gacttcttcg atcccttcct gctgctcgac gaaatgggtc cctcggagct
      721 cgggcccggc gaggcgaagg gcgcgccgga ccaccctcac cgcggcttcg agacggtgac
      781 atacctgctc tccggcgcga tgcagcacaa ggactcgcag ggcaaccagg gcaagttgac
      841 gcccggcgac gtgcagtgga tgacggccgg ttccggggtc gtgcactccg agatgcccga
      901 gccttcgttc cagaagaccg gcgggcgcat gcacgggttc cagctctggg tgaatcttcc
      961 ggcgcgggac aagatgacga agccgcgcta ccaggagatc cccggagcgg gaatcccggt
     1021 cgcgaagagc gaggacggga aggtcgccgt gcgcgtcatc gcaggagagg cgctcggaaa
     1081 gcgcgcggtc atcgagacgc ggaccccgat cttctacctc gactgcacgc tccagccggg
     1141 cgcatcgctg acgcaaccgg ctccggcgga gtacaacacc ttcgcgttcg tgtacgcggg
     1201 tcgtggatcc ttcggatcga acgccagccc cactcgagtg cacgaggcgg tcatctttga
     1261 ggacgacggc gacacggtgc ggctcgaagc cgcagccgac gctccgctgc aggcgctcct
     1321 gatcggtggc gttcctttga acgagccagt cgcgcggtac gggcccttcg tgatgaacac
     1381 gcgcgacgaa atcgtgcagg cgttcgccga ctaccagagc ggcaagctgg gcgagatctc
     1441 cgggccaggt cttcactgac acggagggca ttgggcgagg cgaagtgccc ttgtcaccct
     1501 tgcgaaagca aagtggcaag tggagacctg accccaggcg ctctggacgc cgattgctcc
     1561 cgcgccgtag accttcgcga ggaggaacgg atgtccagac gaatcgggat cgcggtcggt
     1621 ctctcggttg ccgtctttct gccggcggtc gcggatgcgg aatcgtcccg ccccgtccaa
     1681 ccgagcacgt ttctcacgct cggcggtgcc tacaccgtcg aagccggagg ctgcgagcgc
     1741 cactcgcgca acttccgggt ctcttcgcga cgccccatcg acatctcgaa agtcgagaag
     1801 gggcagatcg tgagcggcgt ccacttcaag gagcgtgagc gcgccggacg cgccggctgg
     1861 cgcaacgtca gcgtctcttc ggacggccgg gccgtcgact tcgagctctt cgccgagggc
     1921 gccggccata tccaatcggt cccctcggaa gggaggcagt gcgtcgaggc ggcgccggcg
     1981 cgtgtcgtcg tggacgtcga ggcctgggtc gtggatccct gaatcggcgg gtctgcgaat
     2041 ctggctgacc ctccccggca tcgtaagatt ctgcgaaagt cggaatcacc agaaggaggg
     2101 cccgcatgta caagacgaag ccatgcggtg attgcgcaat gccgccggcc gaaagtccgc
     2161 tgaaggtgct cgttctgaat gcgtcgttga aacgttcccc ggagatttcg aacacgggcg
     2221 aggtggccca gctcgtcctc gacttcatga agccgtacgg catccaggga gagatgatcc
     2281 ggctcgcaga ccacatgatt cccacggggc tcggttttcg ggaaggcccg gacgacgact
     2341 ggccgaagat cgtcggcaag atcaaggcgt ccgaggcttt gatctttgcg acgccgatct
     2401 ggtggggcgg caggtccagc ctgatgcagc gcgttatcga acgccccgac tccctcgacg
     2461 aggagtacat cgcaagcggg cgcagcgccc tctacaacaa ggtcgccggg gtcgtgatca
     2521 ccggaagcga ggacggggcg ctctcgaccc tggggtcgat catgatggtg ctgacgttca
     2581 tgggcttcac gctgccgccg gagtgcgcgg cgtactgggt cggcgaggtc ggcaaccctc
     2641 caagccagga tcgcgacagg cggcttcgca acaaggcgac tccccacatg gcgaagaaca
     2701 tggcgcgcaa cctcgtgtac tacgcgcggc tcctgaacca acacccgctc gtaaccgaac
     2761 ggggggaggc tgcggcagag gtctagctgc tagctctccg ccctctctgt tcttcccgac
     2821 gctccgtgct cccgggctgg gcctccgccg gcctttttcg ccgccgagtg tgcgacagcg
     2881 aacgcacggc tctcgtcgtg cccatactcc tcgtacgcgt tgttgaacgc ctcgaggaag
     2941 gccttcttct ggtgcggcgt gtgctggtcg acctgcgctt ccagcaggtc ggagactctc
     3001 gagtcgggca tatcgacctc cgacg
//
LOCUS       DASWAV010000280         2018 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U2665
            k141_333961, whole genome shotgun sequence.
ACCESSION   DASWAV010000280 DASWAV010000000
VERSION     DASWAV010000280.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960316
            Sequence Read Archive: SRR10260007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2018)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2018)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:47:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,929
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2018
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_333961"
                     /isolate="SMAG_U2665"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.36444 N 132.31889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..202
                     /locus_tag="VF958_05740"
     CDS             <1..202
                     /locus_tag="VF958_05740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9148649.1"
                     /translation="GATKSEIVREAVKLAARRRQRSRIAARPHDAFRGIIGSVRGGPP
                     GLSERTGERFRKLLLDRKRKRS"
     gene            202..603
                     /locus_tag="VF958_05745"
     CDS             202..603
                     /locus_tag="VF958_05745"
                     /inference="COORDINATES: protein motif:HMM:NF013970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HEX9148650.1"
                     /translation="MLVDAGPLVALIHADDRHHASCRDALEQIRESLATVWPVITEAM
                     YLLDFSRQAQDALWRLLERDAVKILPLDAADVPRMRELMRKYRDLPMDLADAALVRVA
                     ERERVSRVFTIDRRDFEVYRPRGIRRFTILP"
     gene            628..729
                     /locus_tag="VF958_05750"
                     /pseudo
     CDS             628..729
                     /locus_tag="VF958_05750"
                     /inference="COORDINATES: protein motif:HMM:NF024242.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
     gene            complement(817..1563)
                     /locus_tag="VF958_05755"
     CDS             complement(817..1563)
                     /locus_tag="VF958_05755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9148651.1"
                     /translation="MGIRDPIPVDVQKVGQGRAEALVSVNRPAQPGFSVGNRVGGSGT
                     IGCVVQDRATSNQFGLTCAHVLAPVPTASTGDRVLVPSLTEARASHVLGRAPMGSLEQ
                     FLPPMFSDSAVAENVDVATFSPTNPATLDSRIAIVGKRPSGVASTVTVGMPVRKVGAS
                     SELTTGEVKFIHMIFWLAYPTPTGEEVTAGFQDLIGISHFSNFGDSGSLVLTEDGKAV
                     GVVLGSTPEFTACLPIQRALDALNCDLVTE"
BASE COUNT          361 a          652 c          665 g          340 t
ORIGIN      
        1 gggggcgacg aagtccgaga tcgttcggga ggcggtgaag ctggcggccc gcaggcgcca
       61 acggtcacgg atcgccgcgc gtcctcatga tgctttccgg gggatcatcg gatccgtgcg
      121 gggcggccct cccgggctgt ccgaacgaac cggcgaacgg tttcggaagc tcctcctcga
      181 ccgcaagcgg aagcgctcct gatcctcgtc gacgcgggtc ccctggtggc cctcattcac
      241 gcggacgacc gccatcacgc ctcctgccgg gatgcgctcg aacagatccg ggagtcgctt
      301 gccaccgtct ggcccgtaat tacggaagcc atgtacctgc tcgacttctc ccggcaagcg
      361 caggacgcac tctggcggct cctcgaaagg gacgccgtca agatcctgcc gctggacgcc
      421 gcggacgttc ccagaatgcg cgagctcatg cggaaatatc gggacctgcc gatggatctc
      481 gcagacgcag cgttggtccg ggtcgcggag cgagaaaggg tctcgcgcgt cttcacgatc
      541 gaccggaggg atttcgaggt ttaccgtcct cgaggaatcc ggcggttcac gatcctgccc
      601 tgaagtccga gacccgacat gctcgagatg agccaggccg gcgtcgccga gatcgcgaag
      661 cgcgtcggct atgacgccga ggccgcgttc tccaaagcgt tcaagcgctg gatcggcgtg
      721 gccccgggcg cgtatcgccg cacggcggaa tcgcggaggc atccggcgct tcgctcggcg
      781 ggcggaggtc ctcaggaggc tcagtcacag gaacgttcat tcggtcacca gatcgcagtt
      841 cagcgcatcc aaggcgcgct ggattggcag gcaagcggtg aactccgggg tgctaccgag
      901 aacgacgccg acggccttcc catcctcggt caagacgagg ctgcccgaat cgccgaaatt
      961 ggagaaatgg gatatcccga tcaggtcttg aaaccctgcc gtcacctctt ccccggtggg
     1021 ggtcggataa gcgagccaga agatcatgtg aatgaacttc acctctccgg ttgtcagctc
     1081 ggaggaggcg cccacttttc gcaccggcat gccaacggtg acggtggagg cgacgcccga
     1141 gggtcgcttt ccgacgattg cgatcctgga atccagagtc gccgggttgg tcggcgagaa
     1201 agtcgcaacg tccacatttt cggcgaccgc ggaatcgctg aacatcggag gcaggaactg
     1261 ttcgagagag cccatgggag cgcgacccag aacgtgactc gcccgagcct cggtgaggga
     1321 aggcacgagt acccgatcgc ctgtgctggc cgtcggcacc ggagcgagga cgtgcgcgca
     1381 agtgaggccg aactgattgg acgtcgcgcg gtcctggacg acgcatccga tcgttcccga
     1441 gccgccgacc cggttgccca cgctgaaccc gggctgcgcg ggccggttca cgctgaccag
     1501 ggcttcggcc cgcccttgac cgaccttctg gacgtcgaca gggatcggat cgcggatacc
     1561 catcggagaa gcatccaacg cccgaggtac gagacgctcg gcccgaatac cttcgggcgg
     1621 gagctttcgc ttcacgaaga ccaccaaaga gaggggacct tcgcggccgg cggtgacctt
     1681 ccgacccact gcaagcccga tcacgttggg ttgcttccac caggaagcgt ttctctgttg
     1741 cagactcctc gcccacgacg gcggagggga ccgacgcatt ctcgcgcctc ggggcccggg
     1801 aggacccggg agacgatcgc cgaggttgcc gaacgcgatg gcggtgacga ggtttcgtcc
     1861 cgcctcacca ctcagttctt gcatggcttg attttggccg aactcgggac gcacttctga
     1921 atgtcggcct gtgcgcgagc gaccgcggcc gctccggcgg ggtcctcaca ctcgacgttt
     1981 tgaatgtttt tcgagatccc cagcagcccg agcacctc
//
LOCUS       DASWAV010000281         3497 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U2665
            k141_615466, whole genome shotgun sequence.
ACCESSION   DASWAV010000281 DASWAV010000000
VERSION     DASWAV010000281.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960316
            Sequence Read Archive: SRR10260007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 3497)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3497)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:47:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,929
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3497
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_615466"
                     /isolate="SMAG_U2665"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.36444 N 132.31889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..487)
                     /locus_tag="VF958_05760"
     CDS             complement(<1..487)
                     /locus_tag="VF958_05760"
                     /inference="COORDINATES: protein motif:HMM:NF012795.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-phosphate kinase"
                     /protein_id="HEX9148652.1"
                     /translation="MKPEDRFVESLRAELAGAARLIVGPGDDAAVIGMEGGPVAVTTD
                     TLVEGIDFLPGEPPARLGRRAVSVALSDLAAMGAWPDFILLTLGLPSSTAPDFPLAVS
                     LAAARRAGEFGAVLAGGDLSKATDLFLTVAAWGRPPGDPILRSTARPGDALFVSGWPG
                     EA"
     gene            complement(552..3071)
                     /gene="lon"
                     /locus_tag="VF958_05765"
     CDS             complement(552..3071)
                     /gene="lon"
                     /locus_tag="VF958_05765"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014552114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HEX9148653.1"
                     /translation="MTEIEDKEAITAAEREADLPIPDILPVLPLKDVVVFPYIILPLS
                     VSREKSINAVDAALAEQRIIMLVAQRNSQIETPRLPDLYPVGTVAAIMRMLKLPDGRT
                     RLLVQGVTRARIDSILSEEPYLKVKITRLEEPEPPKELPPELEALVRSVKQNLEKSVA
                     LGKSISPEVMVIAANLSDPARLADLAASNLELKLEDAQRILESTDPIERLRLVNENLS
                     REITVLSMQQEISTQARGEMDRSQREYYLRQQLRAIQQELGEGEEIAEELEGYRKLVE
                     EKKISAEAAAEIEKQIKRLERSHPDSAETAIIRTYLDWMTGLPWGKLSADNENIDRAR
                     EILDEDHYDIEKVKERILEFLAVHALKKTLKGPILCFVGPPGVGKTSLGKSIARALDR
                     KFVRLSLGGVRDEAEIRGHRRTYVGAMPGRIVQGIHQAGTSNPVFMLDEIDKIGSDYR
                     GDPSSALLEVLDPEQNFSFRDHYLGVPYDLSRVLFIATANMLDPIQPAFLDRMEVIRL
                     SGYTLEEKVAIARRHLIPKQLDENGISDASLEFTEGGIRKIIEAYTREAGLRNHEREI
                     GAICRKVAVAVAKGKTRRYRITGSSVERMLGAVRHFADELLKHDEVGVATGLAWTSVG
                     GDVLFVEALAVRGKGGLRLTGQLGDVMKESAQAAMSYARAHAAGLGIPADFFETHDVH
                     VHVPEGSIPKDGPSAGITMAAAMISAFTHRKVRRDVAMTGEITLRGEVLPIGGVKEKV
                     LAARQARITTIILPKLNRRDVLQVGPKVLHGIVFRYVENVDEVLAIALLPPESEPVES
                     DSEAAVSPPAPEGREIPVQPGPADRRPAARARSGKPEAGSR"
     gene            complement(3068..>3497)
                     /locus_tag="VF958_05770"
     CDS             complement(3068..>3497)
                     /locus_tag="VF958_05770"
                     /inference="COORDINATES: protein motif:HMM:NF012241.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Hsp20 family protein"
                     /protein_id="HEX9148654.1"
                     /translation="RLQREVNRLFTAFVESNNPVVSPASGWDPSVDIVEDGEKVRVLF
                     ELPGVEAADVKVTVRGRVVTVRGNKRGRTRPREGLRFFCMERYFGTFVKSVPLPRPVN
                     TRQAKSRLSRGLLEVVLPRVPDLREKEVEIAVKTEEDAEK"
BASE COUNT          543 a         1170 c         1173 g          611 t
ORIGIN      
        1 cggcttctcc gggccatccc gagacgaaga gcgcgtctcc cggccgggcg gtggagcgca
       61 ggatcgggtc tcctggcggg cgaccccagg cggcgaccgt caggaacagg tcggtggcct
      121 ttgacaagtc tcccccggcg agcacggctc cgaattcacc ggcgcggcgc gcggcggcga
      181 gggacactgc cagcggaaag tcgggcgccg tcgacgacgg caaaccgagc gtcaacagaa
      241 tgaagtccgg ccaggcgccc atggccgcca ggtcggagag tgcgacggac accgctcggc
      301 gtcccagccg agccggaggt tcccccggga gaaagtcgat gccctcgacg agggtatccg
      361 tcgtgacggc aaccggaccg ccctccatgc cgatcaccgc ggcgtcgtcc cccggtccca
      421 cgatcaggcg cgcggcgccg gcgagctcgg cgcgaaggct ttcgacgaag cgatcctcgg
      481 gcttcacggc gcgcgattat cagtcgggag ccggcagcgg gtcgccggga gatgaagatc
      541 cttcggtccc gctaccggct tccggcttcc ggcttgccgc ttcgggcccg cgccgccggt
      601 cgtcggtcgg ccggtccggg ctgcaccggg atctcccggc cctcgggcgc cggaggactc
      661 actgccgcct cgctgtcgga ttcgaccggt tccgactcgg gcggcaggag cgcgatcgcg
      721 agcacctcgt cgacgttctc gacgtagcgg aacacgatcc catggaggac cttcggtccg
      781 acctgaagga cgtcgcgccg attcagtttc ggaaggatga tcgtcgtgat cctcgcctgc
      841 cgcgccgcga ggaccttctc cttcacgccg ccgatcggca gcacctcgcc gcgcagcgtg
      901 atctcgccgg tcatcgcgac gtcgcgccgc accttgcggt gcgtgaatgc cgagatcatc
      961 gcggcggcca tcgtgatccc ggcggagggg ccgtccttgg ggatcgaacc ctcgggcacg
     1021 tgaacgtgga cgtcgtgggt ctcgaagaag tcggccggaa tcccgagccc ggccgcgtgc
     1081 gcccgcgcgt aagacatggc ggcctgcgcg gattccttca tgacgtcacc cagctgaccc
     1141 gtcaggcgca gcccgccctt cccgcggacc gcgagggcct ccacgaagag gacgtcgccg
     1201 ccgacggacg tccacgcgag tccggtggcg actccgacct cgtcgtgctt cagcaattcg
     1261 tcggcgaagt gacgcaccgc gccgagcatt cgctcgaccg acgatcccgt gatgcggtat
     1321 cggcgcgtct ttcccttcgc caccgcgacg gcgaccttgc ggcagatggc cccgatttcc
     1381 cgctcgtggt tgcgcagacc ggcctcgcgc gtgtacgcct cgatgatctt ccgaatgcct
     1441 ccctcggtga actcgaggct cgcgtcggag atgccgttct catccagctg cttggggatc
     1501 aagtggcgcc gcgcgatcgc gaccttttct tcgagggtgt agccggagag gcggatgact
     1561 tccatgcggt cgagaaacgc gggctggatc gggtcgagca tgttcgccgt cgcgatgaag
     1621 agcaccctcg agagatcgta cggaacgccc aggtagtgat cgcggaacga gaagttctgt
     1681 tccgggtcga ggacttcgag cagcgcggag ctcggatccc cccggtaatc cgaaccgatc
     1741 ttgtcgatct cgtcgagcat gaagaccggg ttcgacgtcc ccgcctggtg gatcccctga
     1801 acgatccggc ccggcatcgc cccgacgtag gtgcggcggt gcccgcggat ctccgcctcg
     1861 tcgcgaacgc ccccgagcga caggcgcacg aacttccggt cgagtgcgcg ggcgatcgac
     1921 ttgccgagag acgtctttcc gactccgggc gggccgacga agcagaggat cggccccttc
     1981 agcgtcttct tgagcgcgtg gacggcgaga aactcgagga tgcgctcctt gaccttctcg
     2041 atgtcgtagt gatcctcgtc caggatttca cgggcacgat cgatgttctc gttgtccgcc
     2101 gagagttttc cccacggaag ccccgtcatc cagtcgaggt aggtgcggat gatcgccgtc
     2161 tcggcggaat ccgggtggct tctctcgagg cgcttgatct gtttctcgat ctccgccgcg
     2221 gcctcggccg agatcttctt ctcctcgacc agcttccgat atccctcgag ctcctcggcg
     2281 atctcctcgc cctcgccgag ctcctgctgg atggcgcgga gctgctggcg aaggtagtac
     2341 tcgcgttgcg accggtccat ctcgccgcgg gcctgggtcg aaatctcctg ctgcatggag
     2401 agaaccgtga tctctcgcga aaggttttcg ttcacgagac gcaggcgttc gatcgggtcg
     2461 gtcgattcga ggatgcgctg cgcgtcctcc agcttcagct ccaggttcga cgcggcgaga
     2521 tcggcgaggc gcgcgggatc ggagagattg gcggcgatga ccatgacctc cggcgagatg
     2581 ctcttcccga gcgccaccga cttctcgagg ttctgcttga cgctgcgcac gagcgcctcg
     2641 agctcgggcg ggagctcctt cggcggctcg ggctcctcca gccgcgtgat cttcaccttg
     2701 aggtagggct cttccgagag gatcgagtcg atccgcgcgc gcgtgacgcc ctggacgagc
     2761 agacgcgtgc ggccgtcggg cagcttcagc atccgcatga tcgccgcgac ggtcccgacg
     2821 ggataaaggt cgggcagccg cggcgtctcg atctgggagt tgcgctgcgc gacgagcatg
     2881 atgatgcgct gctcggcgag ggcggcatcc accgcgttga tcgacttctc tcgggaaacg
     2941 gagagcggaa ggatgatgta aggaaagacg acgacgtctt tcaacgggag aacggggagg
     3001 atgtcgggaa tcggaaggtc cgcctcgcgc tcggcggccg tgatcgcttc cttgtcctcg
     3061 atctccgtca cttctccgca tcctcttcgg tcttgacggc gatctccacc tctttttccc
     3121 gcaggtcggg gacccgcggc agcacgacct cgaggagacc ccgggagagc ctcgatttcg
     3181 cctggcgagt gttgacggga cggggcagtg gcaccgactt cacgaaggtt ccgaagtaac
     3241 gctccatgca gaagaatcgc agcccctcgc ggggtcgggt ccggccccgc ttgttgccgc
     3301 gaaccgtcac gacgcggccg cgaaccgtca ccttgacgtc tgcggcctcg acgccgggca
     3361 gctcgaaaag gacgcggact ttctctccat cctcgacgat gtcgacactc gggtcccagc
     3421 cggaggccgg agagacgacc gggttgttgc tctcgacgaa cgcggtgaag agacggttca
     3481 cctcgcgctg aagccgg
//
LOCUS       DASWAV010000282         3617 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U2665
            k141_16075, whole genome shotgun sequence.
ACCESSION   DASWAV010000282 DASWAV010000000
VERSION     DASWAV010000282.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960316
            Sequence Read Archive: SRR10260007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 3617)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3617)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:47:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,929
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3617
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_16075"
                     /isolate="SMAG_U2665"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.36444 N 132.31889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            193..450
                     /locus_tag="VF958_05775"
     CDS             193..450
                     /locus_tag="VF958_05775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008602706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HEX9148655.1"
                     /translation="MGILGWILFGLVVGIVAKLVMPGRDPGGIIVTILLGIVGALLGG
                     FIGQALHLYGPGEPAGFIGATLGAILVLWVYRIAMRRRITA"
     gene            813..1070
                     /locus_tag="VF958_05780"
     CDS             813..1070
                     /locus_tag="VF958_05780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019461902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HEX9148656.1"
                     /translation="MGIIGWILFGLVVGVIAKLIMPGKDPGGFIVTILIGIVGALLGG
                     FLGRLLNLYEPGQPAGFIGATLGAILLLWIYRLATRRKTAV"
     gene            complement(1089..2516)
                     /locus_tag="VF958_05785"
     CDS             complement(1089..2516)
                     /locus_tag="VF958_05785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012872779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HEX9148657.1"
                     /translation="MSDGESVLFTPLRQLAAKVRARKVSPVALAEESLRRLDTLGRKL
                     NAVVTLAGDDALAAAREAEAEIRRGKYRGPLHGIPYGVKDLLATKKMPTTWGASPYRN
                     QQFSYDATVVRKLADAGAVLVAKLSMVELAGGMGYNNADASFTGPGLNPWNTSFWSGG
                     SSSGPGSAVAAGLVPFAIGSETVGSIITPAAFSGVTGLRPTYGLVSRHGAMALCWTLD
                     KLGPLARGADDCAMVLAAIAGKDPADPTTVAAPLSARPPRARPRIAVLKGATDFVQPE
                     VAKNFDDSIAVLGRFADITRDVALADLPYGMCAGTIVDAEGGSAFLELIESGGTRDLQ
                     AKSDRYGGYSSVMTPAVDYLQAMRLREKMRAPMAALFQKYDALAAPSRASVSYPVGID
                     FDKAWPELAKDRPANFVSPIGSLIQVGNLVGWPAISLPNGFGQQGLPTGLHLLGGPLR
                     EADIVAIGSAYQGRTDFHVRRPPGS"
     gene            complement(2513..2821)
                     /locus_tag="VF958_05790"
     CDS             complement(2513..2821)
                     /locus_tag="VF958_05790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9148658.1"
                     /translation="MPRTRRAILKAAGALSALAVLPLPAQTPPPVPPGPTPTATPKKE
                     PGTLAKLARERYGKFLTEEELALMDDDMAALERRSARLRGYKLGNGEEPAVDFAAARR
                     "
     gene            complement(2856..3185)
                     /locus_tag="VF958_05795"
     CDS             complement(2856..3185)
                     /locus_tag="VF958_05795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9148659.1"
                     /translation="MKSKRPLRVFAALLLASVACNDDDGPTGPERGRLFRVRVGAEEF
                     RMRLTDPQTIELAMENLQGRNSKHPNGSLATGDGGFNAPWSWHFVPDSVRMVDSSIGA
                     TAPGALA"
     gene            complement(3163..>3617)
                     /locus_tag="VF958_05800"
     CDS             complement(3163..>3617)
                     /locus_tag="VF958_05800"
                     /inference="COORDINATES: protein motif:HMM:NF027964.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="saccharopine dehydrogenase C-terminal
                     domain-containing protein"
                     /protein_id="HEX9148660.1"
                     /translation="RYPGHAEKMELLRGTGFFDAQPLELRGGVRVTPREVSEQLLLRA
                     WLRPESAEEFTYLKVVVTGRKLGRTLRTTFELLDKTHGGLTSMARTTAFPCAVIARML
                     ARGEYSDPGIRPLEMLASNREAAARLEMGLAARGIRWTKESHEVEASA"
BASE COUNT          568 a         1209 c         1222 g          618 t
ORIGIN      
        1 ggcggggtgt actaggggtc aggtctccac ttgccacatt gcttcgtccg ccagcgtggc
       61 gatgcggtgc ggccgcgctc gaatcagcag tctcgcggcc gcggatgagc gcggcacaaa
      121 ctcccgactc caaagggttt acaagatggc ccgcggcttg ctctttcccg atccggaaag
      181 gaggggaaaa aaatgggaat cctgggttgg atccttttcg gcctcgttgt tggaatcgtc
      241 gcgaaactcg tcatgcccgg ccgcgatccg ggtggaatca tcgtcaccat cctcctcgga
      301 atcgtcggcg cgctcctggg cggcttcatc ggtcaggctc ttcacctgta cggtccgggc
      361 gaaccggcag gcttcatcgg cgcgacgctc ggagcgatcc tcgttctgtg ggtgtatcgc
      421 attgcgatgc gccgtcggat caccgcctga cagatgtgag cgcgcgaagt gctgtctgaa
      481 tctggcactg ggccgcgacc gcggcccttt ttttcatttt ggggttgccc gacccgatgc
      541 gttttgaaat aactcgatcc gtgaaacgaa agaagaaatc acacaggcgt tcggcggaag
      601 accgccggac gaatggcgat cgccgcacga ccagccgccg cgttcacagc cgcgtggtcg
      661 tcggcgggtc cgcgagcgat cggcggaaaa ccgatcggcg ggagcgaccg caaaagccgt
      721 aagcctccgc atgtccccgg acctggcgct tccggcaacg tccggcgggg tcgggctcct
      781 tgttattctc ccgtgccgcg aagggaggga ccatggggat tatcggatgg atactcttcg
      841 gcctcgtcgt cggcgtcatt gccaagctca tcatgcccgg aaaggacccg ggtggattca
      901 tcgtgaccat tctcatcgga atcgtcggtg cgctactcgg aggattcctg ggccgactgc
      961 tgaacctcta cgagcccgga caaccggcgg gcttcatcgg tgcgactctc ggcgcgatcc
     1021 tcctcctctg gatctacagg ctcgccaccc ggcgcaagac cgcggtgtga tgcagcgctg
     1081 atggcgcgtc aggagccagg cggacgccgc acgtggaagt ccgtccggcc ctggtacgca
     1141 cttccgatcg cgacgatgtc ggcctctcga agcgggcctc ccaaaaggtg aagcccggtc
     1201 gggagtccct gctgcccgaa accgttgggc agtgatatcg ccggccagcc gacgaggttt
     1261 cccacctgga tgagcgagcc gatcggcgaa acgaagtttg ccggccggtc cttcgcgagc
     1321 tccggccagg ccttgtcaaa gtcgatgccg acgggatacg agacactcgc gcgcgacggc
     1381 gccgcgagcg cgtcgtactt ctggaacagt gccgccatcg gcgcgcgcat cttctcgcgc
     1441 aaccgcatcg cctggaggta gtccaccgcc ggcgtcatga cgctcgagta gcccccgtag
     1501 cggtccgact tcgcctggag gtcccgcgtg ccccccgact cgatcagctc gaggaaagcg
     1561 ctcccccctt cggcgtcgac gatcgtgccg gcgcacatcc cgtaggggag atcggcgagc
     1621 gcgacgtcgc gcgtgatgtc cgcaaatcgg ccgaggacgg cgatcgagtc atcgaagttc
     1681 ttcgcgactt ccggctgcac gaaatccgtc gcccctttga ggacagcgat ccggggtcgc
     1741 gcgcgcggcg ggcgcgcgga gagcggcgcg gccaccgtcg tcggatccgc agggtccttt
     1801 cccgcgattg ccgcgagaac catggcgcaa tcgtcggcgc cccgcgcgag gggcccgagc
     1861 ttgtcgagcg tccagcagag cgccattgcg ccatgccggc tcacgagccc gtaagtcggg
     1921 cggaggcccg tcacgccgct gaacgcggcg ggcgtgatga tcgagccaac ggtttccgag
     1981 ccgatcgcga acgggacgag accggcggcg accgccgagc cgggcccgct cgacgaaccg
     2041 ccgctccaga aggacgtgtt ccaggggttc agccccggtc ccgtgaacga cgcgtccgca
     2101 ttgttgtagc ccatgccgcc tgcgagctcg accatcgaga gcttcgcgac gagcaccgct
     2161 cccgcgtccg ccagcttccg gacgacggtt gcgtcgtagc tgaactgctg gttgcgatag
     2221 ggtgacgcgc cccaggtcgt cggcatcttc ttcgttgcga ggaggtcttt gacgccgtag
     2281 ggaatgccgt gaagcggccc acggtacttc ccccgccgga tctccgcctc cgcttcgcgc
     2341 gccgccgcga gcgcgtcgtc gccggccagc gtgaccaccg cgttcagctt ccgtccgagc
     2401 gtgtcgagcc gacggagcga ctcctcggcg agggcgacgg gagaaacctt ccgcgcgcgg
     2461 accttcgcgg cgagctgccg gaggggagtg aagaggaccg actcgccgtc gctcatcgcc
     2521 gcgccgcggc gaagtcgacc gccggctcct cgccgttgcc gagcttgtag ccgcgaagcc
     2581 gtgcgcttcg ccgctcgagc gccgccatgt cgtcgtccat gagcgccagc tcctcttccg
     2641 tgaggaactt gccatagcgc tcgcgtgcga gcttcgcgag ggtgcccggc tctttcttcg
     2701 gcgtcgccgt cggagtcggg cccggcggca ccggcggagg ggtctgggcc gggagcggca
     2761 gtacggcgag cgcggagagc gctcccgcgg ctttgagaat tgcccttcgg gttcgcggca
     2821 tctttctcct cctgtattcg actactcgac ggcgatcacg cgagcgctcc aggggcagta
     2881 gccccgatgg acgagtcgac catgcggacg gagtccggga cgaagtgcca gctccaggga
     2941 gcgttgaagc cgccgtcccc ggtggcgagg gaaccgttcg ggtgcttcga gttgcgcccc
     3001 tggaggtttt ccatcgcaag ctcgatcgtc tgaggatccg tgaggcgcat gcggaactcc
     3061 tccgcaccaa cgcgcacgcg aaacagccgc ccgcgctcgg gcccggtcgg gccgtcgtcg
     3121 tcgttgcagg cgaccgacgc gagaagcagc gccgcgaaga ccctaagcgg acgcttcgac
     3181 ttcatggctc tctttggtcc agcgaattcc ccgcgccgcc agtcccatct ccagccgggc
     3241 cgcggcctct cgattggagg cgagcatttc gagggggcga atgcccggat cgctgtattc
     3301 gccgcgcgcg agcatgcggg cgattacggc gcaggggaat gccgtcgtgc gggccatcga
     3361 ggtcagcccg ccgtgagtct tgtcgaggag ctcgaaggtc gtgcgcagag tgcgcccgag
     3421 cttgcggccc gtcacgacca ctttgaggta cgtgaattcc tcggcgctct ccgggcggag
     3481 ccacgcgcgc aagagaagtt gctcggacac ctcgcgaggc gtcacgcgta cgcctccgcg
     3541 gagctcgaga ggctgcgcgt cgaagaagcc ggtcccgcgc aggagctcca tcttttcggc
     3601 gtgcccggga tatcgca
//
LOCUS       DASWAV010000283         2901 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U2665
            k141_54106, whole genome shotgun sequence.
ACCESSION   DASWAV010000283 DASWAV010000000
VERSION     DASWAV010000283.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960316
            Sequence Read Archive: SRR10260007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2901)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2901)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:47:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,929
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2901
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_54106"
                     /isolate="SMAG_U2665"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.36444 N 132.31889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..156)
                     /locus_tag="VF958_05805"
     CDS             complement(<1..156)
                     /locus_tag="VF958_05805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015744659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX9148661.1"
                     /translation="MATDSSRRSVMALGWVSFLTDVASDMIYPLLPAFLTKTLGAGPA
                     TVGLIEGV"
     gene            235..978
                     /gene="recO"
                     /locus_tag="VF958_05810"
     CDS             235..978
                     /gene="recO"
                     /locus_tag="VF958_05810"
                     /inference="COORDINATES: protein motif:HMM:TIGR00613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HEX9148662.1"
                     /translation="MGLASADAFILGTYPLKEKDRIVSFLTRESGRRRGVAKRARGAR
                     SNFTGSLEPMTEVRVVFFERDGRELVSINSIDPVRPSFPLSKDLERALLLSALAESLE
                     TFVSDSDPAEPFFRLARHAMDSLFAGAPAKTVAAYFDVWILKLSGLFPSPAECAGCGR
                     PLESTEALLFDESRPGFVGAECRRGEVLRLSPEAATTLRAFLSRPLDPEGSPRAVGEL
                     VTVARRARRSFLGHELKSQRVLAEVLGSS"
     gene            975..1352
                     /locus_tag="VF958_05815"
     CDS             975..1352
                     /locus_tag="VF958_05815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019341457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HEX9148663.1"
                     /translation="MKLGLTVLPGLYSVVRLAPGAKAPRGLTGFHSITRTERELSIVC
                     RAGEGPRARRREDGFRCLEVAGPFAFDAVGVLARLAAPLARARVPILAISTFDTDYLL
                     VREARLPGAVRALERAGHRVRVE"
     gene            1363..2253
                     /locus_tag="VF958_05820"
     CDS             1363..2253
                     /locus_tag="VF958_05820"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014016840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HEX9148664.1"
                     /translation="MLTLQRVIAELCAFWESEGCLIEQPYDQPVGAGTMHPATFLRVL
                     GPDPWRCAYVQPSRRPADGRYGQNPFRLAKHLQLQVILKPSPDDVQQVYLRSLERLGI
                     VAADHDIRFEEDNWESPTLGAWGVGWQVVMDGMEITQFTYFQQAGGVDLAPISAEITY
                     GLERIAMFLNKVDSIYEVPWTESIKYGEVRRVEEEELSKYYFEVADTALTALLLDQYE
                     TEAKRALAAGLVIPAYEATLACSQQFNVLDARGAVSATERVGLIRRVRDLACACARAY
                     VSSRGGSPTPVTEPEPAATV"
     gene            2321..>2901
                     /locus_tag="VF958_05825"
     CDS             2321..>2901
                     /locus_tag="VF958_05825"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: protein motif:HMM:NF014183.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HEX9148665.1"
                     /translation="MKRDDFLFEIRTEEIPAASLRGAREDLARGIGDALAQEGLAPDS
                     VESYATPRRLIVWARGVPERQPDRETEVLGPPAAAAFDAAGHATRAAEGFARAQKVEV
                     SDLVVVDSPRGKTVAARRTVAGRSASELLGEVVPRVVDGLSFPKTMRWGTGERSFVRP
                     VRGVLALYGGDVVPMDVMGMRAGDSTAGHRVLS"
BASE COUNT          422 a          978 c         1014 g          487 t
ORIGIN      
        1 gacgccctcg atcaacccga ccgtggccgg gcccgctccg agcgtcttcg tcaggaaggc
       61 cggaaggagc gggtagatca tgtcggaggc cacgtcggtc aggaacgaga cccaccccag
      121 ggccatgacc gaacggcgcg acgagtcggt tgccatattt tattcggatt atttagagat
      181 tttccttatt tcattcgggc cgttggaccg accggtggct atcataatcg gaccgtggga
      241 cttgcctccg ccgacgcgtt catcctcggg acctatccgc tcaaggagaa ggaccggatc
      301 gtctccttcc tgacgcggga gtccggccgg cggcgcggcg tcgcgaagag ggcacgcggc
      361 gcgcggagca acttcacggg gtcgctcgag cccatgaccg aagtccgggt ggtcttcttc
      421 gagcgcgacg ggcgcgagct cgtttcgatc aactcgatcg acccggtgcg gccgtctttc
      481 cctctctcga aagatctcga gcgggcgctc ctgctgtcgg cgctggcgga gagcctggag
      541 accttcgtct cggactccga tccggccgag cctttcttcc ggctcgcacg gcacgcgatg
      601 gattcgctct tcgccggtgc gccggcgaag accgtggccg cctacttcga cgtgtggatc
      661 ctgaaactct ccggcctgtt tccgtcgccc gccgagtgcg ccggctgcgg gcgaccgctc
      721 gaatccacgg aggcgctcct gttcgacgag agccggcccg gcttcgtcgg ggcggagtgc
      781 cggaggggag aagtgttgcg gctgtcgccg gaggctgcga cgactctgcg ggcctttctc
      841 tcgcgcccgc tcgatcccga aggttcgccc cgcgcggtcg gagagctcgt caccgtcgcc
      901 cgccgcgccc gccggagctt tctcggacac gagttgaagt cgcagcgcgt cctggccgaa
      961 gtgctcggaa gctcttgaag ctcgggctca ccgtcctgcc gggactctat tcggtcgtcc
     1021 ggctcgcgcc cggagcgaag gcgccccggg gactgacggg gtttcactcg atcacccgca
     1081 cggagaggga gctctcgatc gtctgccgcg cgggagaagg cccgcgggcg cggcggaggg
     1141 aagacggctt ccgctgtctc gaggtcgcgg gcccgttcgc gttcgacgcg gtgggggtgc
     1201 tcgcgaggct cgcggcgccg ctcgcccggg cgcgcgttcc gatcctcgcg atctcgacgt
     1261 tcgacaccga ctacctcctg gtgcgcgagg cgcggcttcc cggggcggtt cgcgcgctcg
     1321 agcgcgccgg tcaccgcgtg cgcgtggagt agactttcgg cgatgctgac gctccagagg
     1381 gtgatcgcgg aactctgcgc gttctgggag tcggaaggct gcctgatcga gcagccgtac
     1441 gaccagccgg tcggcgccgg aacgatgcac cccgcgactt tcctccgcgt gctcggtccc
     1501 gatccgtggc gatgcgccta cgttcagccg tcgcgccgac ccgccgacgg acggtacgga
     1561 cagaatccgt tccgcctcgc caagcacctg cagctgcagg tcatcctgaa gccttctccc
     1621 gacgacgtgc agcaggttta cctgcgcagc ctggagcggc tcggaatcgt cgccgcggat
     1681 cacgacatcc gcttcgagga ggacaactgg gagtcgccga cactcggggc ctggggggtc
     1741 ggctggcagg tcgtgatgga cggaatggag atcacccagt tcacttactt ccagcaggcg
     1801 gggggcgtgg atctggcgcc gatctcggca gagatcacgt acggcctcga gcgcatcgcg
     1861 atgtttctga acaaggtgga ctcgatctac gaggtcccct ggaccgaatc gatcaagtac
     1921 ggagaggtgc ggcgcgtcga ggaggaagag ctctcgaagt actacttcga ggtcgccgac
     1981 actgcgctca ccgccctcct cctcgaccag tacgagaccg aagcgaagcg cgcgctcgcc
     2041 gcgggcctgg tgatcccggc ctacgaggcg acactcgcgt gttcgcagca gttcaacgtg
     2101 ctcgacgcgc gcggggccgt ctccgccacc gaacgagtcg gcctgatccg gcgcgttcgg
     2161 gatctggctt gcgcgtgcgc ccgcgcttac gtttcgagcc gtggaggatc tccgacgccg
     2221 gtcaccgaac ccgagcctgc ggcgaccgtc tgatcacacg gttgcaccgc gagcggggtt
     2281 ccgccagaca ccgctaagat tgcgcggcga acttcttttc atgaaacgcg acgacttcct
     2341 tttcgagatc cggacggagg agattcccgc cgcgtccctg cggggagcga gggaggacct
     2401 cgcgcgcgga atcggcgacg cgctcgcgca ggaaggcctt gcgccggact ccgtcgagtc
     2461 ctacgcgacc ccgcgccgcc tcatcgtgtg ggcccgaggc gtgcccgagc gccagccgga
     2521 ccgcgagacg gaagtgctcg gaccgccggc ggcggcggcg ttcgacgccg caggccacgc
     2581 gacgcgagcc gccgagggct tcgcgcgggc gcagaaagtg gaggtctccg atctcgtcgt
     2641 cgtcgactcg ccccgcggca agaccgtcgc cgcgcgtcgg accgtcgcgg gccgatccgc
     2701 gtccgaactg ctcggggagg tcgttccgcg ggtggtcgac ggactctcgt ttccgaaaac
     2761 gatgcgctgg ggcaccggcg agcgctcgtt cgtgcggccg gtgcgcggcg tgctcgcact
     2821 ctacggcggg gacgtcgttc cgatggacgt gatggggatg cgcgcgggcg actcgaccgc
     2881 cggccaccgg gtgctctccg a
//
LOCUS       DASWAV010000284         3519 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U2665
            k141_535565, whole genome shotgun sequence.
ACCESSION   DASWAV010000284 DASWAV010000000
VERSION     DASWAV010000284.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960316
            Sequence Read Archive: SRR10260007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 3519)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3519)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:47:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,929
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3519
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_535565"
                     /isolate="SMAG_U2665"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.36444 N 132.31889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1032)
                     /locus_tag="VF958_05830"
     CDS             complement(<1..1032)
                     /locus_tag="VF958_05830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9148666.1"
                     /translation="MQRCIRATVLAACAVFWVASMRAQTPPPEGLIIRGFISATAFAQ
                     DQNFTFGNGQNAEWPAPPEFTRDKWFAGGDVRNTRLTLALNGPKVGKAGWAVGGQLEM
                     DFFGGFNGTGAFSEQQPVPRLRLAFADIGNGRTTIRIGQYWSPLFGFTSNAAASVVPV
                     SLSHIAFPLGYGTGMPGWRFPGIFLYQGLTPKTSPVNAELQLAAMRGSWDRCVGGTVN
                     GDCPTGGANSNINSLSAGNASWPQFEGRVNLTGKAGKGTWGVYVVGHYDKKDLTGANA
                     EAPNDSLTGWIGEFGAKYTMGGFLLQGNVYTSQGAGQQFTAISQFGAWRNESGDARPI
                     QSTGGWVQAG"
     gene            complement(1263..2114)
                     /locus_tag="VF958_05835"
     CDS             complement(1263..2114)
                     /locus_tag="VF958_05835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012527225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEX9148667.1"
                     /translation="MATGIRAQRLGAKTFQWTVLGVFTLLLAFPFYWMLITSFKRTSD
                     LYNLENNPFIFNAKPTLEHLRLLFRETLFLRWLGNTAFAGLLVVVITLLLAVPAAYAL
                     ARLTGRWGSRLGIGIFLTYLVPPTLLFIPLSRIVAWLHLQDTLWAIIVVYPSFTIPFS
                     TWLLMGFFKSIPPELEDAAMVDGLTRFQAFLKLIIPVSVSGILTVVIFTFTLVTQEFV
                     YALTFISSAAHQTVGVGVPIYLVRGDVYYWGSLMAACLIASLPTAILYNLFLDRFIAG
                     FTVGSVK"
     gene            complement(2114..2998)
                     /locus_tag="VF958_05840"
     CDS             complement(2114..2998)
                     /locus_tag="VF958_05840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011985973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEX9148668.1"
                     /translation="MSGLRQRWLGPAMFLPAVLYVVALVGVPFVLAFLYSVGDVKVGS
                     VGYHFVGLKNFASVFQNPTFLLALRNSFVFTITSQVLVLIGANLLALALKDAFRGRRF
                     LRFLILLPWVAPVSIGSIGWKWILDSLYSVINWVLVRLHFVKPFAAPMWLGEPKLAML
                     SVILVHTWHMLPFATVILLAGWTAIPKDIPEAAAVDGAGFFRRMFQIEIPMMLPIINV
                     AVLFGVIFTFTDMTVIYILTRGGPYDTTQVLPSMAFFAGILGSDLAEGAAISIFLVPL
                     LVAVAWLMLRSAHRAEVT"
     gene            complement(2995..>3519)
                     /locus_tag="VF958_05845"
     CDS             complement(2995..>3519)
                     /locus_tag="VF958_05845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015934459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX9148669.1"
                     /translation="AREELEQFQKKIGTTTIYVTHDQVEAMAMGDRVVVMNHGSVRQI
                     GSPADVYDEPADTFVATFLGSPPMNLIETPSVIAGFRPEHFYPANALPRDEPVLRLAF
                     LISHEEYLGAERILYGVLKGGLFDGKKVVSRMSSTLAARFAPDQVHEFAVAEKDLKFF
                     DREAGTRTGRVALE"
BASE COUNT          736 a         1062 c         1158 g          563 t
ORIGIN      
        1 cccggcctgg acccatccgc cggtgctctg gatcggtcgg gcatctcccg actcgttgcg
       61 ccaggcgccg aactggctga tcgccgtgaa ctgctggccg gccccctggg acgtgtagac
      121 gttgccctgg agcaggaaac cgcccatcgt atatttcgcg ccgaactcgc cgatccagcc
      181 ggtgagcgag tcgttgggcg cctcggcgtt ggcgccggtc aggtccttct tgtcatagtg
      241 gccgacgacg tagacacccc aggttccctt cccggccttt ccggtcaggt tgacgcggcc
      301 ctcgaactgc ggccagctcg cgtttcccgc ggagagcgag ttgatgttcg agttcgcgcc
      361 gccggtcggg cagtcaccgt tgaccgtgcc cccgacgcag cgatcccagc taccgcgcat
      421 cgcggcgagc tggagttccg cgttgaccgg ggaggtcttc ggggtcagcc cctggtagag
      481 gaagatcccg gggaaccgcc agccgggcat gccggttccg tagccgagtg gaaacgcgat
      541 gtgcgagagc gagacgggca caacgctcgc cgcggcgttc gaggtgaaac cgaagagcgg
      601 cgaccagtac tgtccgatgc ggatcgtcgt ccggccattg ccgatgtcag caaacgcgag
      661 acgcagacgg gggaccggct gctgctcgga gaatgcgcca gtgccgttga agccgccgaa
      721 gaagtccatc tcgagctgcc cgccgacggc ccagccggcc ttcccgacct tcggcccgtt
      781 gagcgccagc gtcagacggg tgttgcggac gtcaccgcca gcgaaccact tgtcccgcgt
      841 gaactccgga ggagcgggcc actccgcgtt ttgcccgttc ccgaaggtga agttctggtc
      901 ctgagcgaac gcagtggcgc tgatgaaacc ccggatgatg agaccctccg gaggcggggt
      961 ttgagcgcgc atcgaggcga cccagaagac ggcacacgcc gcgaggaccg tggcacgaat
     1021 acatcgctgc atcgaagtct cctttctaat caaggcttac gcgccgacct tgccggagcg
     1081 tgatgccatt gcatcaagat cgagtgcctg ggaaggaaac gcggctattt ctcgcggatc
     1141 ggccgggtcg gggaaagagg ggagcgaggg gtgcagagcg acctcggaag gaactttcca
     1201 cttcccttcc cgaggagacc cgtcgacatc gagacatatc gtcggaaatc acccctattg
     1261 tgtcacttta ccgacccgac tgtgaacccg gcgatgaatc ggtcgagaaa aaggttgtag
     1321 aggatggccg tcgggaggct cgcaatcaga caggcggcca tgagcgaacc ccagtagtag
     1381 acgtcccccc gaacgaggta gatcggcacc cccacgccga ccgtctggtg agccgcggag
     1441 gaaatgaacg tcagggcata gacgaactcc tgcgtgacca gcgtgaacgt gaaaatcacg
     1501 acggtcagga ttccggagac cgaaaccggg atgatgagct tcaaaaaggc ctgaaatcgc
     1561 gtgaggccgt ccaccatggc cgcgtcctcg agctccggag ggatcgactt gaaaaagccc
     1621 atcagaagcc aggtcgagaa cgggatggtg aagctcgggt agacgacgat gatcgcccac
     1681 agcgtgtcct ggagatggag ccacgcaacg atacgcgaga gcgggatgaa gagcagcgtc
     1741 ggaggtacca gatatgtcag gaagatcccg atgccgagcc gacttcccca gcggccggtc
     1801 aggcgcgcga gcgcgtaggc cgccggcacg gcgagcaata gcgtgatcac caccacgaga
     1861 agccctgcga acgccgtgtt gccgagccaa cgcaggaaaa gcgtctcacg aaagaggagg
     1921 cggaggtgct cgagagtcgg tttcgcgttg aatatgaatg gattgttctc gaggttgtag
     1981 aggtcggatg ttcgcttgaa gctcgtgatg agcatccagt agaaggggaa agcgagcaac
     2041 agggtgaata caccgagcac ggtccactgg aaggtcttgg cgcccagccg ctgcgcgcgg
     2101 atgccggtcg ccatcacgtc acctccgcgc ggtgcgccga tcgcagcatc agccaggcga
     2161 ccgcgacgag cagcggcacg aggaagatgg aaatcgccgc cccctcggcg aggtcgctgc
     2221 cgaggattcc cgcaaaaaag gccatcgacg gcagcacctg cgtcgtgtcg tagggccctc
     2281 cccgggtcag gatgtagatg accgtcatgt cggtgaaggt gaagatcacc ccgaagagga
     2341 ctgccacgtt gatgatcggc agcatcatcg gaatttcgat ctggaacatt cttcggaaga
     2401 acccggcccc gtcgacggcc gcagcttccg gaatgtcttt cgggatcgcg gtccacccgg
     2461 cgaggagaat gaccgtggcg aacgggagca tgtgccaggt gtggacgagg attaccgaca
     2521 gcatcgcgag cttgggctcg cccaaccaca tcggcgcggc gaagggcttg acgaagtgaa
     2581 ggcggacgag aacccagttg atgacgctgt agagcgaatc gaggatccat ttccagccga
     2641 tggagccgat cgaaacgggc gccacccagg gaagcaggat gagaaagcgc aagaaccgcc
     2701 ggccccgaaa ggcgtccttc agcgcgagcg cgagcaggtt ggctccgatc aggaccagca
     2761 cctgggacgt gatcgtgaaa acgaacgagt ttcgaagcgc gagaagaaac gtcggattct
     2821 ggaatacgga ggcgaagttc ttcaggccga cgaagtgata gccgacgctt ccgaccttaa
     2881 cgtccccgac gctgtagagg aacgcgagca cgaaaggaac gccgaccagg gcgacgacgt
     2941 agaggaccgc cggcagaaac atcgccggtc cgagccagcg ttgccgcagg ccgctcactc
     3001 gagcgccacc cgccccgttc gggtgccggc ttcccggtca aagaacttca agtccttttc
     3061 cgccaccgcg aactcgtgaa cctggtcggg cgcaaaccgc gcggcgagcg tggaagacat
     3121 cctcgacacg actttcttgc cgtcgaagag gccgcccttg agcactccgt aaaggatccg
     3181 ctccgccccc agatattcct cgtggctgat gaggaacgcg agtcgcagga cgggctcatc
     3241 tcgcggcaga gcgttcgccg gatagaagtg ctcgggtcga aaaccggcga tcacgctcgg
     3301 ggtctcgatc aaattcatcg gaggggagcc gaggaaagtt gccacgaaag tatccgccgg
     3361 ctcgtcgtag acgtcggccg ggctgccgat ttgccggacg gaaccgtggt tcatcacgac
     3421 gacccggtcg cccatcgcca ttgcctcgac ctggtcgtgt gtgacgtaga tcgtggtggt
     3481 tccgatcttc ttctggaact gctcgagctc ctcacgggc
//
LOCUS       DASWAV010000285         3558 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U2665
            k141_456237, whole genome shotgun sequence.
ACCESSION   DASWAV010000285 DASWAV010000000
VERSION     DASWAV010000285.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960316
            Sequence Read Archive: SRR10260007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 3558)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3558)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:47:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,929
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3558
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_456237"
                     /isolate="SMAG_U2665"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.36444 N 132.31889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1925)
                     /locus_tag="VF958_05850"
     CDS             complement(<1..1925)
                     /locus_tag="VF958_05850"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019517697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M13-type metalloendopeptidase"
                     /protein_id="HEX9148670.1"
                     /translation="MISKLAASAISLTLLAPVARAAEPAATTATTAPTTAQRYGTWGV
                     DLAAMDRSVKPGDDFFRYVNGKWAATTQIPPDKTGYGAFVILHDLSEARERALLDRWA
                     ADKNLKAGSDEAKVAAIYRTFLDEKTPEKLDAKPIQAHLDAIRKAESREDVAILMARS
                     QAGFGRSFFGAGVSDDAKNPDKYALYVSQSGLGLADREFYLQDKFKAQKDRYQKYIAD
                     MLRLIGWGEPEKSAADIVALETKIAEAHWTRAESRNRDKTYNPATVAELEKSAPGFPW
                     RVYFKEAEIDKADRAVVRQNTALPKLAKIFADTPVATLKAWEAFSVADDAAPYLSKRF
                     VETNWEFRSKFLNGTEEQRPRWKRAVDAAEGAMGEAIGRTYVAEYFPPESKAKMEKLV
                     ADLRAAMKGRIENLQWMGPETKARALEKLSKFGVKIGYPSKWRDYSALVIREGDLAGN
                     AERAAKFEWEYDVNRIGKPVDELEWGMTPQTVNAYYSPVKNEIVFPAAILQPPFFDPN
                     ADAAVNYGAIGGVIGHEITHGFDDQGRKSDGDGMLRDWWSPEDAAKFEAQAAKLGAQY
                     EAFNFPQLPGLHITGRLTMGENIGDLGGILLGLDAYKVSLNGQPAPVLDGFTGEQRVL
                     LGWGQVWRTIMRDDALR"
     gene            2141..3208
                     /locus_tag="VF958_05855"
     CDS             2141..3208
                     /locus_tag="VF958_05855"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: protein motif:HMM:NF012382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004400 - histidinol-phosphate
                     transaminase activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HEX9148671.1"
                     /translation="MMPILLDRNENRYGPAPQCLAVLKEATPELLFNYTRDFQRGYYS
                     ELSRKLAEMHGVPEKRIVLGYGCEDLLKQAIHHWIRRGDRCLIPSASWWYYRSIADEV
                     GGVTVEYPILEQPTTYSYDIEALLRIQRETPARVLLISSPNNPTGNLFPRRRLEEMLE
                     QFRDAIVVYDEAYWGFSREQPEDLTAMTQSHPNLLVLRTFSKLYALAGARIGYGIAGA
                     GLDSFLKFSARNLGYNRLSERLALAALSNPGYYESIRVHMVTDRQTILDALRGFPGIR
                     AYDSDANFVLARFPAAAVAQVKADLDRRGLIVKFFTEPAFVNCARITLGTPKENASLV
                     AAFQEILGRPGVLDALASSAS"
BASE COUNT          572 a         1179 c         1121 g          686 t
ORIGIN      
        1 tggcgcagcg catcgtcgcg catgatcgtt cgccacacct gcccccagcc cagtaacacg
       61 cgctgctcgc cggtgaagcc gtcgagcacc ggagcgggct gaccgttcag ggagacctta
      121 tacgcgtcga gccccagaag gatgccgccg aggtcgccga tgttctcgcc catcgtgagg
      181 cggcccgtga tgtggaggcc gggcagctgc gggaagttga atgcttcgta ttgcgcgccg
      241 agcttcgccg cctgcgcctc gaacttggcc gcatcttccg gcgaccacca gtcgcgcagc
      301 atgccgtcgc cgtcggactt ccgtccctga tcgtcgaagc cgtgcgtgat ctcgtggccg
      361 atgacgccgc cgatcgcgcc gtagttcacc gccgcatcgg cgttcggatc gaagaagggc
      421 ggctgcagga tcgccgcggg gaagacgatc tcgttcttca ccggggagta gtaggcgttg
      481 acggtctggg gagtcatgcc ccactccagc tcgtcgacgg gcttgccgat ccggttcacg
      541 tcgtattccc actcgaattt cgcggcgcgc tcggcgttgc cggcgaggtc tccctcgcgg
      601 atcacgaggg ccgagtaatc acgccacttc gacggatagc cgatcttcac gccgaacttc
      661 gacagcttct cgagcgcgcg tgccttcgtc tccggtccca tccactggag gttctcgata
      721 cggcccttca tcgcggcgcg caggtccgcg acgagcttct ccatcttcgc cttcgactcc
      781 ggggggaagt actccgcgac gtaggtccgg ccgatcgctt cgcccatcgc gccttccgca
      841 gcgtcgacgg cccgcttcca gcgcggacgc tgctcctccg tgccgttcag gaacttcgag
      901 cggaactccc agttcgtttc gacgaagcgc ttcgaaaggt acggagccgc gtcatcggcg
      961 acgctgaatg cctcccacgc tttgagcgtc gcaaccggcg tgtcggcgaa gatcttcgcg
     1021 agctttggca acgcggtgtt ctgacgaacg acggcacggt cggccttgtc gatttccgct
     1081 tccttgaaat agacgcgcca tgggaatccc ggggcgctct tctcgagttc cgcgacggtc
     1141 gcggggttgt acgtcttgtc gcgattccgg ctctcggcgc gcgtccagtg cgcctcagcg
     1201 atcttcgtct ccagcgcaac gatgtccgcg gcgctcttct ccggctcccc ccatccgatc
     1261 aagcggagca tgtcggcgat gtacttctgg tagcggtcct tctgcgcttt gaatttgtcc
     1321 tggaggtaga actcgcgatc tgcgaggccg aggccggact gcgaaacgta gagcgcgtac
     1381 ttgtccgggt tcttcgcatc gtccgagacg ccggcgccga agaacgaccg gccgaagccg
     1441 gcctgagagc gcgccatgag gatggcaacg tcttcgcgcg actccgcctt ccggatcgcg
     1501 tcgaggtgcg cctggatcgg ttttgcgtcc agcttctccg gggtcttttc gtcgaggaac
     1561 gtgcggtaga tcgccgcgac cttcgcttcg tccgaaccgg ccttcagatt cttgtccgcc
     1621 gcccagcggt cgagaagagc gcgttcccgc gcctcggaga gatcgtgcag gatcacgaac
     1681 gccccgtaac ccgtcttgtc cggggggatc tgggtcgtcg cggcccactt gccgttgacg
     1741 tagcggaaga agtcgtcgcc gggcttcacc gagcgatcca tcgcggcgag atcgactccc
     1801 cacgtgccgt agcgctgggc cgtcgttgga gcggtcgtcg cggtcgtggc agcgggttcg
     1861 gcggcgcgcg caaccggtgc gagcagagtc agcgaaatcg cggaagcagc gagttttgag
     1921 atcatgaaat gatttccttt tctgaaggcc aagcccgtcc gaaaggacgc gagaatagca
     1981 aaatcggggg cgggagaccg ccgcagggcc gttcatcgca gcgtcatgtt tttgatatcg
     2041 ccatgtgggg gcaggagaag cccaaggccc cggcgtcata agctctccct gtgattgccg
     2101 gggtggaatg cattcgggtg agggggcgct tttgcacgag atgatgccga tccttctcga
     2161 tcgcaacgag aaccgatacg ggccggcccc gcaatgcctc gccgtcctga aggaggcgac
     2221 gccggagctg ctcttcaact acacgcgcga cttccagagg ggctactaca gcgaactctc
     2281 gcggaagctc gcggagatgc acggcgttcc cgagaaacgc atcgttctcg ggtacggatg
     2341 cgaggacctc ctgaagcagg ccatccatca ctggatccgg cggggcgacc gctgcctcat
     2401 tccgagcgcc tcctggtggt actaccgctc gatcgcggac gaggtcggcg gtgtgacggt
     2461 cgaatacccg atcctcgagc aaccgaccac ctacagttac gacatcgaag ctcttctccg
     2521 gattcagaga gagactcccg cccgagtcct cctgatctct tcccccaaca acccgacggg
     2581 caatcttttt ccgcggcggc gtctggagga gatgctcgag caattccggg acgcgatcgt
     2641 cgtttacgac gaagcgtact ggggtttctc ccgggaacaa cccgaagacc tgacggccat
     2701 gacgcagtcc catccgaatc tgctggtctt gcggacgttc tcgaagctct atgcgctcgc
     2761 cggcgcccgg atcggttatg gcatcgccgg tgccgggctc gacagcttcc tgaagttctc
     2821 cgcgcgcaac ctgggataca accggctctc ggagcggctc gcgctcgcgg cgctgtcgaa
     2881 ccccggttac tacgagagca tccgggtcca catggtcacc gaccgacaga cgatcctcga
     2941 tgccctgcgc ggttttcccg ggatccgggc ctacgattcc gacgccaact tcgttctcgc
     3001 gcgcttcccg gcagccgccg tcgcgcaggt gaaagcggac ctcgaccgcc ggggcctgat
     3061 cgtcaagttc ttcacggagc cggcgttcgt caattgcgcg cggatcaccc tcgggacccc
     3121 gaaggaaaac gcgagtctcg tcgccgcctt ccaggagatt ctgggccgtc ccggcgttct
     3181 cgatgcgctc gcttcgtccg cgagttagca gacgaggttc cggaaacgtc tcacgagtgg
     3241 ctctccgcct accggcgatc tctcaaggcg ccggagctcg aggagcggct ggacctctgg
     3301 atctaccgac ccctcgcgtt cctggtcgca tggcctctgc gcaagaccgc ggttcgcccc
     3361 aaccacgtca gcctcgcgag tctcgcctgc ggggtcgcgg cgggattttc gttcggcagc
     3421 gcgacatggg ccggcgccat tctcggggcg atcgcctact ttttcgggaa cgtgctcgat
     3481 tgcgccgacg gtcagctcgc gcgactccag agctccgcat ctccgatcgg atatctcgtg
     3541 gacggagccg cggactac
//
LOCUS       DASWAV010000286         2818 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U2665
            k141_54186, whole genome shotgun sequence.
ACCESSION   DASWAV010000286 DASWAV010000000
VERSION     DASWAV010000286.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960316
            Sequence Read Archive: SRR10260007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2818)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2818)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:47:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,929
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2818
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_54186"
                     /isolate="SMAG_U2665"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.36444 N 132.31889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2484
                     /locus_tag="VF958_05860"
     CDS             <1..2484
                     /locus_tag="VF958_05860"
                     /inference="COORDINATES: protein motif:HMM:NF027232.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HEX9148672.1"
                     /translation="IMRRFSTAFLAALALFFAVAAPAAVAPATPDKAKTFSDQLAGMD
                     FRCIGPYRGGRVTAVAGARHDPLTYYFGGTGGGVWKTTDGGSNWESVSDKTFKTGSVG
                     AIAVSESDPNVVYVGMGESHVRGNVSHGDGVYKSTDGGHTWKNVGLRDTRQIARVRID
                     PADPDLVYVAALGHIWGANAERGVFRSRDGGRTWSKVLSVDEKTGASDLAMDPTNPRI
                     LYAGFWQVVRRPWELVSGGPGSSLWRSTDGGDTWKKLSLPDNEGLPEGIWGKVGVAAS
                     AAKPGRVYAFIEAGRGGLFVSEDYGEKWKHVNDEHKIRERAWYYSWVYADPENADTVY
                     LPNVYMHKSTDGGRSFSNLKVPHGDNHDLWIDPDDPDRMILGNDGGATITFNGGQTWS
                     TQNNQPTAQFYRVITDNQFPYWVYGSQQDNSNVAIPSGVVGSEGIDATDWHAAGGGES
                     GWMAPDPTDPQIVYAGEYGGQITRYDHRTRQARQIMAWPQLADGHATKDLKYRFQWNA
                     PILISPNDPRALYHASQKLLRSRDGGETWEEISPDLTRNDPAKQGKSGGPITIDVTGV
                     EVYDTIFALAESKLEPGVIWAGTDDGLVHVTRDNGKTWQNVTPAAMPEWIQVNAIDLS
                     AHQKGTAYVAATRYKFDDFHPYLYKTTDYGKSWARIDRGIPEDAFTRVVREDPGQRGL
                     LFAGTETGLYVSTDDGASWKPFQRNLPAVPITDLTFKDGDLVVATQGRSFWILDDLTP
                     LRNWSDSMAGETAHLFTPRPAVRIQTQKRDEEEELPAPIGSNIPNGVVVDYWLKEKPG
                     RGEKVTLEFLAGDKVIRTFSSEKGSRKAT"
     gene            2571..>2818
                     /locus_tag="VF958_05865"
     CDS             2571..>2818
                     /locus_tag="VF958_05865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9148673.1"
                     /translation="MRVFKPTLVPKAVFNEGEKAPPKVGPGVYKVRLTAGGRSLTETF
                     EVRPHPAGFATAEDLKAQYDLLSAIRDRLSETHATVAAV"
BASE COUNT          553 a          908 c          944 g          413 t
ORIGIN      
        1 ataatgagac gcttctcaac ggctttcctc gcagctctgg ctctcttctt tgcggtggcg
       61 gcgcccgcag ccgttgcccc tgccaccccg gacaaggcca agacgttttc cgaccagctc
      121 gccggcatgg atttccgctg catcggtccg taccgcggcg ggcgtgtcac ggctgtcgcg
      181 ggagcgcggc acgacccgct gacgtactac ttcggcggta ccggcggcgg cgtctggaag
      241 acgaccgacg gcggctcgaa ctgggagtcc gtctcggaca agacgttcaa gaccggctcg
      301 gtcggcgcga tcgcggtgtc agagtcggac ccgaacgtcg tgtacgtggg aatgggagag
      361 tcgcacgtcc gcgggaacgt ctcgcacggc gacggcgtgt acaagtcgac cgacggcggg
      421 cacacgtgga agaacgtcgg actccgcgac acccgtcaga tcgcgcgggt ccgcatcgat
      481 cctgcggatc ccgacctcgt ctacgttgcc gcgctggggc acatctgggg cgccaacgcc
      541 gagcggggcg tctttcgatc gagagacggc ggacggacgt ggtcgaaggt cctctcggtc
      601 gacgagaaga cgggcgcctc cgacctcgcc atggacccga ccaatccgcg gatcctctac
      661 gcgggcttct ggcaggtcgt gcggcgtccg tgggagctcg tcagcggagg tccgggaagc
      721 tctctctggc gctcgaccga cggcggtgat acgtggaaga aactctctct tcccgacaac
      781 gagggacttc cggagggaat ctggggaaag gtcggggtcg ccgcctccgc ggcgaagccg
      841 ggccgcgttt atgcgttcat cgaggcgggg aggggcggtc tcttcgtgtc ggaggattac
      901 ggcgagaagt ggaagcacgt caacgacgag cacaagatcc gcgaacgcgc ctggtactac
      961 tcgtgggtct atgccgaccc ggagaatgcc gacacggtct atctgcccaa cgtgtacatg
     1021 cacaagtcga ccgacggcgg aaggagcttc tcgaatctca aggtccctca cggcgacaac
     1081 cacgacctgt ggatcgatcc ggacgacccg gaccgcatga tcctcggcaa cgacggagga
     1141 gcgacgatca ccttcaacgg cggccagacg tggtcgaccc agaacaacca gccgaccgcg
     1201 cagttctacc gggtcatcac ggacaatcag tttccgtact gggtatacgg gagccagcag
     1261 gacaactcga acgtggcgat accgagcggc gtcgtcggga gcgagggcat cgacgccacc
     1321 gactggcacg cggccggcgg cggggagagc ggctggatgg cgcccgaccc gaccgatccc
     1381 cagatcgtct acgcgggcga gtacgggggc cagatcaccc gctacgacca ccgcacgcgt
     1441 caggcgcggc agatcatggc atggccgcag ctcgccgacg gccatgcgac gaaggacctc
     1501 aagtaccgct tccagtggaa cgcgccgatc ctgatctcgc ccaacgatcc gagggcgctc
     1561 taccacgcct cccagaagct cctgcgcagc cgcgacggcg gggaaacgtg ggaggagatc
     1621 tcaccggacc tgacgagaaa cgatccggcg aaacagggca agtcgggcgg accgatcacg
     1681 atcgacgtga cgggagtcga ggtctacgac accatcttcg cgctcgccga gtcgaagctc
     1741 gagccgggcg tgatctgggc cggcaccgac gacgggctcg ttcacgtcac tcgggacaac
     1801 ggaaagacct ggcagaacgt cacgccggcc gcgatgcccg aatggatcca ggtcaatgcg
     1861 atcgatctct cggcccacca gaagggcaca gcctacgttg ccgcgacgag atacaagttc
     1921 gacgacttcc atccctatct ctacaagacc accgactacg ggaagtcctg ggcccgcatc
     1981 gaccggggaa tcccggagga cgccttcacc cgggtcgtgc gcgaggaccc gggccagcgc
     2041 ggcctgctct tcgccggaac cgagaccggc ctgtacgtct cgacggacga cggcgcgtcg
     2101 tggaagcctt tccagagaaa cctcccggct gttcccatca ccgacctgac tttcaaggac
     2161 ggcgacctcg tggtcgcgac ccagggaagg tccttctgga tcctcgatga cctgacgccg
     2221 cttcggaact ggagcgattc catggcggga gagaccgcgc accttttcac tccgcggccc
     2281 gccgtgcgca tccagacgca gaagcgcgac gaggaagagg agctgccggc cccgatcggg
     2341 agcaacattc cgaacggcgt cgtcgtggac tactggttga aagagaagcc gggcagggga
     2401 gaaaaggtca cgctggaatt cctcgccgga gacaaggtca tccgcacctt ctcgagcgag
     2461 aaagggagcc gcaaggcgac ctgaaggaac aggcggcccg cgaggaggag gagaaggaaa
     2521 aggacaagcc actcgagccg aaggcggggg tcaaccgctt cgtgtgggac atgcgggtct
     2581 tcaaacccac gctggtgccg aaggcggtct tcaacgaggg cgagaaagct ccaccgaagg
     2641 tcgggcccgg cgtctacaag gtccggctca ccgcgggcgg ccgttcgctc acggagacgt
     2701 tcgaggtgcg cccccatccc gcgggcttcg cgacggcaga ggatctgaag gcgcagtacg
     2761 accttctctc ggcgatccgt gaccggctct ccgagacgca tgcgacggtc gcagcggt
//
LOCUS       DASWAV010000287        10002 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U2665
            k141_95615, whole genome shotgun sequence.
ACCESSION   DASWAV010000287 DASWAV010000000
VERSION     DASWAV010000287.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960316
            Sequence Read Archive: SRR10260007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 10002)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10002)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:47:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,929
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10002
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_95615"
                     /isolate="SMAG_U2665"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.36444 N 132.31889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..725
                     /locus_tag="VF958_05870"
     CDS             <1..725
                     /locus_tag="VF958_05870"
                     /inference="COORDINATES: protein motif:HMM:NF014755.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006303 - double-strand break repair via
                     nonhomologous end joining [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="Ku protein"
                     /protein_id="HEX9148674.1"
                     /translation="KDMAPIRYKKFCSTEDVEVPNDEIVKGYEVSKKKFAIVEAEELD
                     EVQEEVGEGNRAIDVLQFVEFSSLNPLSFEKPYYLAPQKGGEKAYAVLRDALADANRV
                     GIARFYLRTRPLLAALIPGKKALALEVLRSFEELRDPADLSIPASEKKSAEVKMARLL
                     IDQMSAEEWDPTKHPNEYRRALEKLLSSKRKFALKETKEPENVVDLMEALRRSVGKAG
                     GATGRARPKKSARTPTRRAGAA"
     gene            739..969
                     /locus_tag="VF958_05875"
     CDS             739..969
                     /locus_tag="VF958_05875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9148675.1"
                     /translation="MRREVALTGGAALAAGLLLGLIADRLAHFFPSAVTGRPDPAPPM
                     ADRCDDGRQRPAPVETRLVTDVLEPYRVAAAV"
     gene            975..1901
                     /locus_tag="VF958_05880"
     CDS             975..1901
                     /locus_tag="VF958_05880"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEX9148676.1"
                     /translation="MKRRVGSQRRRRIRRVLLRALRRSFPHLGRFTPSLSARLAEEIF
                     RTPPKHARLRREEHALAGARFSATPSSRGALPTWTWGEGPSVLLAHGWGGHSGRLTPF
                     VRPLVEKGFSVVAFDAPGHGSAPGRYSSLPDFVEAIRTVARSHGPFEAMIGHSLGAAA
                     CALAVRDGVPVSRIVLLAPPAEAEKYTGRFARFFGIPPATHAEMKRRLERRYRTRWSD
                     LRVAGGHCPVRALVLHDPRDGKVPFRDGQEVVRTWPNAELVQTRGVGHHRILSNPQVI
                     AHAVSFVSSAAEPPLRARRPRRARLGAAAFAS"
     gene            1986..3410
                     /locus_tag="VF958_05885"
     CDS             1986..3410
                     /locus_tag="VF958_05885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019462896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mercuric reductase"
                     /protein_id="HEX9148677.1"
                     /translation="MRSRATPGRRRRNVAEKPADYDVLIIGAGQAGPPLAIALAKKGW
                     SVAIAERKRLGGSCVNFGCTPTKAVIASARVAHLARRGRSFGVRIGSVEPDLAAVLDR
                     ARGIVNQSRMGLREALANEMNLRLIEGHARFDGRDGKSFRLHVGEETVHARKVVIDTG
                     TRSRIPSVHGLENIDYRTAENWLDRPLLPRHLLVLGGSYTGLEMAQFYRRMGSAVTVV
                     EEGPEIVRREDPDVSLALRRLLEAEEIAFRLGHAAARVEAVPDGIRLSFRHGGGRETL
                     AGTDLFVATGRRPNTDDLGLETIGLEPEKDGTIRVTKRLASKVRGVWAVGDVRGGPMF
                     THTAWDDYRILLSQLTGDRSRTTDRIVPYAIYTDPQLGRVGMTETEAKSRGRRVRVGR
                     FEMRQNGKAREIGETEGFVKVVVGGSSGRILGAAVLSTEAAELVHLFVDVMNAGGTFA
                     AIRDAIHVHPTLAEAIQSAVASVK"
     gene            3407..4720
                     /locus_tag="VF958_05890"
     CDS             3407..4720
                     /locus_tag="VF958_05890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014764635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucoamylase family protein"
                     /protein_id="HEX9148678.1"
                     /translation="MTRESTPERLTKLLELQRHTFGYFVHEANPANGLVRDNTRYGSP
                     SSIAAVGLALACWPIAVERGIATREEAAQRVLATLRFFDESETGPQPDATGHRGFFYH
                     FLDLTTGRRAHRCELSTMDTAILLAGALTAAAYFERADGEEADIRRLADRLYRAADWE
                     WMLDGDRTVRHGWTPEGGFLRYRWQGYNEALILYALALGSPTHPLPAECYAAWAATYE
                     WKRAYGIDSISGGPLFMHQLSHLWIDFRGIGDAYVREKGIDYFENSRRATLVQQRYAE
                     ANPKRFRGYSKFVWGITASDGPGPAVRRIAGRTRRFYDYCARGVPDGPDDGTLSPWAV
                     VASLPFAPEVVVPTMNALLAKYPAIRTHYGLLCSFNPTFGTAHDGWIAKGYYGLDQGP
                     VVIMIENYATGMVWKLMQRCGPLVEGLRRAGFSGGWLDVEGRPRG"
     gene            complement(5040..5183)
                     /locus_tag="VF958_05895"
     CDS             complement(5040..5183)
                     /locus_tag="VF958_05895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010648309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lmo0937 family membrane protein"
                     /protein_id="HEX9148679.1"
                     /translation="MVWTIFVILLVLWLLGFFAFKVTSGLIHLILVVAVIVLILGLVR
                     RGP"
     gene            complement(5305..5796)
                     /locus_tag="VF958_05900"
     CDS             complement(5305..5796)
                     /locus_tag="VF958_05900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009959016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9148680.1"
                     /translation="MDEQRRLDPNPDANRDPLSGEPGSHPIGTGVGAAGGAATGAAVG
                     GAVGGPVGAVVGGVVGAVAGGAAGHSVAEGLDPTVEDAYWRKSYTTRPYYRAGSAYEE
                     YEPAYRYGWETASRPEYRGREFDAVESDLERGWEKAKGKTREGWRDVKAATRDAWDRV
                     RGH"
     gene            complement(5842..6357)
                     /locus_tag="VF958_05905"
     CDS             complement(5842..6357)
                     /locus_tag="VF958_05905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9148681.1"
                     /translation="MDKSTRAEGTVHRHGGRTHDSEDTSRRGMSTDATAADEAETMAS
                     TGDSENAFQEIAAGTATGWGIDRGESAGTGGLAAGEDLDEVAEGDYWRQNFRRRPYYQ
                     PGTPYEHYEPGYRYGWQNALEGEYEGRDFDDVESELERSWDALHGENAQEWEEARAIV
                     RDAWQRARREK"
     gene            6621..7223
                     /locus_tag="VF958_05910"
     CDS             6621..7223
                     /locus_tag="VF958_05910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9148682.1"
                     /translation="MAGAFSLARVAGRRRSVRLWAGGFAAIGAAAILGGSWHAFSPGL
                     SAPSADFLWKATLAAAGLAGCLLIAGAAFASVPRRGATWVAGAAAIKLAVFLGRALPG
                     NVFDPVVLDSAATLAAILALQIFSFATRRAPSAPWVIAGVVVSAAASAVEAFRPKLLP
                     DPLGPDAVYHLVEIAGLFLFYRGGRLFDDVDVAAQPNTSG"
     gene            complement(7202..8638)
                     /locus_tag="VF958_05915"
     CDS             complement(7202..8638)
                     /locus_tag="VF958_05915"
                     /inference="COORDINATES: protein motif:HMM:NF013132.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008509 - monoatomic anion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006820 - monoatomic anion transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DASS family sodium-coupled anion symporter"
                     /protein_id="HEX9148683.1"
                     /translation="MIFLAAGPAAWLALRFAAPATLSPAAAHVLGLAGWMALWWIGEP
                     VPLAATSLLPLILFPVLGVASAKEAAIPYANELVFLFLAGFLLAAALERWNAHSRIAY
                     AIVGAIGGGGRRVVLGVMLATAFVSMWISNTATAAMMYPIAIAVGALFGTGRAADEGR
                     TALMLGVAYSASIGGIGTLIGTPPNLIFAGAARQLAGREVSFADYMMVGLPIVAILLP
                     LCWFLLVFVLFRRGIDLGADARRTLSARRSALGSLRGGEARTVVVFLVTVAAWLFRER
                     KEIGAFAVPGLADLAPGISDSSVGLAGALLLFVVPDGSSPRASRPLLTWREAKEIPWE
                     VLLLFGGGLSLAAAMESSGLARWLGDRMGGLAGQPPVLVYGGLATLVLVLSELASNTA
                     VAAMAMPIAASLAPAVGEPPLALMLVAALAASTGFALPVATPPNTIVFGSGQVSVRQM
                     ARAGILLDLVAIVVIVAAVSLLYPLVFG"
     gene            complement(8697..9107)
                     /locus_tag="VF958_05920"
     CDS             complement(8697..9107)
                     /locus_tag="VF958_05920"
                     /inference="COORDINATES: protein motif:HMM:NF036460.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5329 family protein"
                     /protein_id="HEX9148684.1"
                     /translation="MKAFRPVCSIVIALLLIPAALFAARRPIRPVAEQTKIDFLLGEV
                     RSSPAIFVRNGSEYPGRSAASHLLSKLNFAGKRVQNARQFITGIASHSEASGKPYEIR
                     WPDGRREALAEWLLERLAIYEREHLPAPETTPLR"
     gene            complement(9104..9451)
                     /locus_tag="VF958_05925"
     CDS             complement(9104..9451)
                     /locus_tag="VF958_05925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020733678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX9148685.1"
                     /translation="MLVVEDDAQLRQSICTLLEDAGYTSRPAENGEIALERAREERPC
                     VILLDLMMPIMNGWEFRSEQLQDPKLSSIPVVIMTADGRAAEKARTLHADYLRKPIHL
                     DALLELVNDYCGT"
     gene            complement(9556..9631)
                     /locus_tag="VF958_05930"
     tRNA            complement(9556..9631)
                     /locus_tag="VF958_05930"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(9596..9598),aa:Lys,seq:ctt)
     gene            complement(9716..9922)
                     /gene="thiS"
                     /locus_tag="VF958_05935"
     CDS             complement(9716..9922)
                     /gene="thiS"
                     /locus_tag="VF958_05935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019461588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur carrier protein ThiS"
                     /protein_id="HEX9148686.1"
                     /translation="MEVRVRVNGNERRFPDGTSIAALLVALGVSTPRVAVERNREIVP
                     KSAYSSTHLLEGDALEVVEFVGGG"
BASE COUNT         1664 a         3396 c         3373 g         1569 t
ORIGIN      
        1 gcaaggacat ggctccgatt cgctacaaga agttctgcag caccgaggac gtcgaggtcc
       61 ccaacgacga aatcgtcaaa ggctacgagg tctcgaagaa gaagtttgcc atcgtcgagg
      121 cggaagagct cgacgaggtc caggaggagg tcggtgaggg gaaccgcgcg atcgacgtcc
      181 tccagttcgt cgagttctcg tcgctgaatc cgctgtcctt cgaaaagccc tactacctcg
      241 ccccgcagaa ggggggcgag aaggcctacg ccgtattgcg cgacgctctg gcggacgcga
      301 accgggtcgg gatcgcgcgg ttctacctcc ggacgcggcc gcttctcgcg gcgctcattc
      361 ccggaaagaa ggcgctcgcg ctcgaagtgt tgcggtcctt cgaggagtta cgcgatcccg
      421 cagatctgtc gatacccgct tcggagaaga agtccgccga ggtgaagatg gcgagactcc
      481 tgatcgacca gatgtcggcg gaagagtggg atccgacgaa gcacccgaac gaatatcgcc
      541 gcgccctcga gaagctcctc tcgtccaagc gcaagttcgc gctgaaggag actaaggagc
      601 ctgagaacgt cgtcgacctc atggaagcgc ttcgccggag cgtcggaaag gccggagggg
      661 caaccggccg cgcccgcccg aagaaatccg ccaggacgcc gacgcgccgg gccggggcgg
      721 cgtagaagga ggatccgcat gcgtcgcgaa gtggcgttga cgggaggcgc cgcccttgcg
      781 gcgggccttc tgctcgggtt aatcgcggat cggcttgcgc atttctttcc gtcagccgtg
      841 accgggcgcc cggatccggc accgccgatg gcggaccgtt gcgatgacgg tcggcaacga
      901 ccggcgccgg tcgaaacccg gctggtcacc gacgtgctcg agccctaccg ggtcgcggcg
      961 gccgtctgag gcgcgtgaaa cgccgggtcg gctctcagcg ccggcgccgg atccgccgcg
     1021 tactcctccg ggccctgcgc cgcagctttc ctcacctcgg gcgattcacg ccctcccttt
     1081 ccgcgcggct ggcggaggag atcttccgga cgccgccgaa gcacgcccgg cttcggcgcg
     1141 aggaacacgc gctcgccggg gcgcgctttt cggccacgcc ctcgagcaga ggcgcgctgc
     1201 cgacctggac gtggggggaa gggccttcgg ttctcctcgc gcacggttgg ggcggacatt
     1261 ccggccggct gactcccttc gtccgcccgc tcgtcgagaa gggcttctcg gtggtcgctt
     1321 tcgacgcgcc gggacatggc agtgctccgg gcagatattc gtcgcttccg gacttcgtcg
     1381 aggcgatccg cacggtcgcg cgaagccacg gaccgttcga ggcgatgatc ggccactccc
     1441 tcggggcggc tgcatgcgcg ctcgccgttc gcgacggggt gccggtatcg cgcatcgtcc
     1501 tcctggcgcc gccggccgaa gcggagaagt acaccggccg cttcgcccgt ttcttcggaa
     1561 tcccgccggc gacgcacgcc gagatgaagc ggcggctcga acggcgatac cgcacccgct
     1621 ggtcggacct tcgggtcgcc ggcggacact gccccgtgcg cgcgctcgtc cttcacgatc
     1681 cgcgtgacgg gaaggtcccg ttccgcgacg gtcaggaggt cgtgcggacg tggccgaatg
     1741 cggagctcgt ccagacgcgg ggcgtcggac accaccggat cctgagcaac ccccaggtca
     1801 tcgcccacgc cgtctccttc gtttccagcg ccgccgagcc gccccttcgc gcgaggcgac
     1861 cccgtcgcgc acggctggga gcggccgcat tcgcatcgtg aaaacttcat aagaggcgcc
     1921 gtctcgcggc tcgcacattg cttccgcaaa aagggcagga agggcacacc aacgaggagg
     1981 tcgacatgag gagccgcgcc acgccaggcc gccggcggag gaacgtagcc gaaaagccgg
     2041 cggactacga cgtcctcatc atcggcgcag gccaggcggg tcctccgctc gcaatcgcgc
     2101 tcgcgaagaa ggggtggagc gtcgccatcg cggagcgcaa acgcctcgga gggtcttgtg
     2161 tgaacttcgg gtgtacgcct acgaaggccg tcatcgcctc ggcgcgggtg gcccacctgg
     2221 cgcggcgcgg ccgctccttc ggagttcgca tcggttccgt cgaaccggac ctcgcggcgg
     2281 tgctcgaccg cgcgcgcggg atcgtgaacc agagccgaat gggcttgcgg gaagcgctcg
     2341 cgaacgaaat gaacctccgg ttgatagagg gacacgcgcg tttcgacggg cgcgatggca
     2401 agtcgtttcg cctgcacgtc ggcgaggaga ccgtgcacgc acggaaggtc gtcatcgata
     2461 ccggaacccg gagccgcatc ccttcagttc acgggctcga gaacatcgat tatcggaccg
     2521 cggagaactg gcttgatcgg ccgctgctcc cgcgccatct cctcgtgctc ggagggagct
     2581 acaccgggct cgagatggcg cagttctatc gccggatggg gagcgcggtc accgtggtgg
     2641 aggaagggcc ggagatcgtc cgacgcgaag accccgacgt ctccctcgcg cttcgacgcc
     2701 ttctcgaagc ggaggagatc gcgtttcggc tcgggcacgc ggcggctcgc gtggaagccg
     2761 tccccgacgg aatccgcctt tcctttcggc acgggggggg ccgcgagact ctcgcgggaa
     2821 ccgacctctt cgtcgcgacg gggcgccgtc cgaatacgga cgatctcggc ctcgagacga
     2881 tcggcctcga gcccgagaag gacggcacga tccgggtgac gaagcgtctc gcgtcgaaag
     2941 ttcgcggcgt ctgggccgtc ggcgacgtcc ggggcggccc gatgttcact cacacggcgt
     3001 gggacgacta tcggatcctc ctttcccagc tcaccggaga ccgatcgcgg acgaccgatc
     3061 ggatcgtgcc gtacgcgatc tacaccgacc cgcagctcgg acgcgtcggg atgaccgaga
     3121 ccgaggcgaa gagcagggga cgcagggtac gcgtcgggcg cttcgagatg cggcagaacg
     3181 gaaaggcgag agagatcgga gagacggaag gattcgtcaa ggtcgtggtc ggagggtcaa
     3241 gtggccgcat cctcggggcg gccgttctct cgaccgaggc cgcggagctc gttcacctct
     3301 tcgtcgacgt gatgaacgcc ggaggcacct tcgccgcgat tcgcgatgcg attcacgtcc
     3361 acccgaccct ggccgaggcg attcagagcg cggtcgccag cgtgaagtga cccgagaatc
     3421 gaccccggag cggctgacga agctcctcga gctgcagagg cacacgttcg gatacttcgt
     3481 ccacgaagca aacccggcaa acgggctcgt tcgtgacaac acgcgctacg gctcgccctc
     3541 ctcgatcgcc gcggtcggcc tcgctctcgc ctgctggccg atcgcagtcg aacgcggaat
     3601 cgccacccgc gaggaggcgg cgcagcgggt gctcgcgacg ttgaggttct tcgacgagag
     3661 cgagaccggg ccgcagccgg acgcgaccgg gcatcgcggt ttcttctacc acttcctcga
     3721 cttgacgacc ggacgaagag cgcaccggtg cgagctgtcg acgatggaca cggcgatcct
     3781 gctcgccgga gcgttgacgg ccgccgcata tttcgaacgc gccgacgggg aggaggccga
     3841 catccgacgg ctcgcggatc gcctgtaccg cgcggccgac tgggaatgga tgctcgacgg
     3901 cgaccggacg gtgcggcacg gatggacgcc ggagggcggg tttctgcgct accggtggca
     3961 ggggtacaac gaggcgctca tcctgtacgc gctcgccctc gggtcgccga cccacccctt
     4021 accggccgag tgctatgccg cgtgggctgc gacctacgag tggaagcgcg cctacggaat
     4081 cgactccatc tccggcggcc cgctcttcat gcaccagctc tcccatctct ggatcgactt
     4141 ccgcgggatc ggcgatgcct acgtgcggga gaagggcatc gactacttcg agaacagccg
     4201 gcgcgcaacc ctcgtgcagc agcgatacgc ggaggcgaac ccgaaacgat ttcgcggcta
     4261 ctcgaagttc gtctggggga tcaccgcgag cgacggtccc ggcccggccg tccggcggat
     4321 cgccgggcga acgcggcgct tctacgacta ttgcgcgcgg ggggtacccg acggtccgga
     4381 cgacgggacg ctgtcgccct gggcggtcgt cgcctcgctt cccttcgcgc ccgaggtcgt
     4441 cgttcccacg atgaacgccc tcctggcgaa gtaccccgcg atccggacgc actacggtct
     4501 tctgtgcagc ttcaacccga cgttcgggac ggcgcacgac ggctggatcg cgaagggcta
     4561 ttacgggctc gaccagggcc cggtcgtgat catgatcgag aactacgcga ccggaatggt
     4621 ctggaagctg atgcagcgct gcggtcccct cgtcgaaggc ctgcggcgcg ccggtttttc
     4681 cgggggatgg ctcgacgtgg aaggtcgacc ccgagggtga ccgagctcct gtcgcggccg
     4741 gggcgcgcgg acgcagcggc cggactcccc gcgggacgag tcagacggcg gcgcagtccg
     4801 ggtgcccgca ggggcaggcc gtgccgtggc ggccggtcgt ttccatctcc gcgcatatct
     4861 cgctgcagaa acgcttcccg ccacgttcga cggtcgtctc atcgcattca catccacggt
     4921 gctcgcaagt catggcgacc tccttcctcg ggacggtcag caaaccggct gccacggttc
     4981 gagagcgagg acgcgcgcgg ctcctctttc gagtcgatcc aggaagccgg ccttcgggcc
     5041 tacggaccgc gtcgaacgag acccaggatg agaacgatga cggcgacgac gagaatcagg
     5101 tggatcaatc cgctcgtcac cttgaacgcg aagaacccga gcagccagag caccagaagg
     5161 atcacgaaga tcgtccagac catagctcct cctttctccc tctcgcgcgc gggattttac
     5221 gttgaccgtg cacagcgtac agaaagggcc cgctttcgcg ggccctggat tccgattgaa
     5281 ccttgaactt cgaacgtgta agggtcagtg tcctcggacc cgatcccacg cgtcgcgggt
     5341 cgcggccttg acgtcgcgcc agccttcgcg ggtcttgccc ttcgccttct cccaaccgcg
     5401 ctcgaggtcc gactccacgg cgtcgaactc gcgcccccgg tattccgggc gagacgcggt
     5461 ctcccagcca taccgatacg ccggctcgta ctcctcgtat gcgctgccgg cgcggtagta
     5521 gggacgcgtg gtgtagctct tgcgccagta ggcgtcttcc acggtggggt cgagtccctc
     5581 ggcgaccgag tggccggccg ctccacccgc gacggcgccg acgactccgc cgacgaccgc
     5641 gccaaccggc ccgccgaccg cgccgccgac cgctgccccc gtggccgctc cgcccgccgc
     5701 cccgactccc gtaccgatcg gatgagaacc cggctcgccg ctcagcggat cgcggttcgc
     5761 gtccggattc gggtcaagtc ttctctgttc gtccatttcg aaacctcctg tcagatgcga
     5821 gagcaagaag cgtaccggcg cctacttctc tcgacgagct cgctgccagg cgtctctcac
     5881 gatcgcgcgc gcttcttccc actcctgtgc gttctctccg tggagggcat cccacgagcg
     5941 ttcgagctcg ctctcgacgt cgtcgaagtc gcgcccctcg tactcccctt cgagggcgtt
     6001 ctgccagccg tagcgatagc ccggctcgta gtgctcgtac ggagttcccg gttgatagta
     6061 cggacggcgg cggaagttct ggcgccagta gtccccttcg gcgacttcgt cgagatcctc
     6121 tcccgccgcg agcccgcccg ttcccgccga ctcgccgcgg tcgatccccc agcccgtcgc
     6181 cgtcccggcc gcgatctcct ggaacgcgtt ctcgctgtcc ccggtcgagg ccatcgtctc
     6241 tgcttcgtcg gccgcggtcg cgtccgtgct cattccgcgg cggctcgtgt cttccgagtc
     6301 gtgcgtgcgt ccgccatgcc ggtggacggt tccctccgcc cgcgtgctct tgtccatgtg
     6361 gcctccctcc cgttatcgag agcaaggggc gtaccggaat cggacgcccg tcgttgcgag
     6421 cggtaaaatc tggcggcctt tccgcaagga gtacatgaag gggcgtacga cgcgccagac
     6481 gtacgaagtt cgctcgggca atgacccgga agatgcagga gaggggcgcg tctccaggga
     6541 cgggaaagaa atcgaagtag tgacccctgg attctgaagc cgtcaccgcg gccaccgatt
     6601 acctcctcgc cctcgccgcg gtggcgggcg cgttctcgct cgcccgtgtg gcgggccggc
     6661 gccgttccgt ccgcctctgg gcgggcggat tcgccgctat cggcgcagcg gcgatcctag
     6721 gcggctcgtg gcacgcattc tcgccggggc tctcggcgcc gtcggcggat tttctctgga
     6781 aggcgacgct cgccgcggcc ggcctcgccg gctgcctgct gatcgccggg gcggcgttcg
     6841 cttcggtccc ccgacgcgga gcgacgtggg tcgccggagc cgccgcgatc aagctcgcgg
     6901 ttttcctcgg acgggcgctc ccgggaaacg tgttcgatcc cgtcgttttg gatagcgccg
     6961 cgacactcgc ggcgatcctg gcgctccaga ttttctcctt cgcgacacgg agagcgccga
     7021 gcgcgccgtg ggtgatcgcg ggcgtcgtcg tgtctgcagc cgcctccgcg gtcgaagcat
     7081 ttcgcccgaa gctgctgccg gatccgctcg ggccggacgc cgtctaccac ctcgtcgaga
     7141 tcgccggcct tttcctcttc taccgcggcg gccggctctt cgacgacgtc gacgtcgcgg
     7201 ctcaaccgaa tacgagcgga tagagcagcg acaccgccgc gacgatcacg acgatcgcca
     7261 cgagatcgag aaggatcccg gccctcgcca tctgccgcac actcacctga cccgacccga
     7321 acacgatcgt gttcggcgga gtagccaccg gaagcgcgaa acccgtcgat gcggcgagcg
     7381 ctgcgaccaa catgagagcg agcggcggtt cgccgacggc gggcgcgagg gaagcggcga
     7441 tcggcatcgc catcgccgcg acggcggtat tcgacgcgag ctccgagaga acgagcacga
     7501 gtgtcgccag ccccccgtag acgagcaccg gcggctggcc cgcgaggccg cccatccggt
     7561 cgcccaacca tcgcgcgagg ccgctcgact ccatcgcggc ggccagcgag agcccaccgc
     7621 cgaaaagcag caaaacttcc cacgggatct ctttcgcctc gcgccacgtg agaagcgggc
     7681 ggctcgcccg cggcgatgat ccgtcgggga ctacgaacag gagaagcgca ccggcgagcc
     7741 cgacggaaga gtcggagatt ccgggcgcga ggtccgcgag cccgggaacc gcgaaggcac
     7801 cgatctcctt gcgctcgcgg aacagccagg cggcgacggt cacgagaaag accacgacgg
     7861 tgcgcgcctc ccctccccgg agcgaaccga gcgccgagcg gcgggccgag agcgtccggc
     7921 gcgcgtccgc gccgaggtcg atcccgcggc ggaagagaac gaagacgagg aggaaccagc
     7981 agagcggcag caggatggca actatgggaa ggccgaccat catgtagtcg gcgaagctga
     8041 cctcgcgccc ggcgagctga cgggcggctc ccgcgaagat caagttcggc ggcgtaccga
     8101 tgagggttcc aattcctccg atcgacgcgg agtacgcgac gccgagcatc agcgccgtgc
     8161 ggccttcgtc ggccgcgcga cccgtgccga agagcgcgcc gaccgcgatc gcaatcgggt
     8221 acatcatcgc cgccgtggcg gtgttcgaga tccacatcga cacgaaagcg gtcgcgagca
     8281 tcaccccgag caccacgcgc cggcctcccc cgccgatcgc accgacgatc gcgtaggcga
     8341 tacgcgagtg cgcgttccac cgttcgagcg cggccgcgag cagaaatccg gcaaggaaaa
     8401 ggaagacgag ctcgttcgcg taagggatcg ccgcttcctt tgcggaggcc acgccgagga
     8461 cgggaaagag gatgagcggc aggagcgagg tcgcggcgag cggcaccggc tcgccgatcc
     8521 accacaaggc catccagccc gccagtccga gaacgtgggc ggcggccggc gagagggtgg
     8581 ccggcgcggc gaagcggagc gcgagccagg ccgcgggtcc ggccgcaagg aagatcatgc
     8641 gacgggtcga gcccggcgca atcgaaacgg aactcaagca gagggaagga tagcggttaa
     8701 cgcagcgggg tcgtctccgg cgcagggaga tgctctcgct cgtagatcgc gagcctttcg
     8761 agcagccatt cggcgagcgc ctcccggcgg ccgtccggcc atcggatctc gtacggtttg
     8821 ccggacgcct ccgagtgcga cgcaatcccc gtgatgaact gtcgtgcgtt ctggacccgc
     8881 ttgccggcga aattcagttt cgagagcagg tgggacgccg cgcttctccc cgggtattcg
     8941 cttccgttgc gaacgaagat cgcgggggaa cttctcacct cgccgagcag gaagtcgatt
     9001 ttcgtctgct cggcgaccgg ccggatcggg cgtctcgcgg cgaagagcgc tgccggaatc
     9061 agcagaagcg caatcacgat cgagcaaacc ggccgaaagg ctttcacgtg ccgcaatagt
     9121 cgttcacgag ctcgagcagg gcgtcgaggt gaatcggctt cctcaggtag tcggcgtgga
     9181 gagtgcgagc cttttccgcg gcgcggccgt ccgcggtcat gatcacgacc gggatcgacg
     9241 acagcttcgg gtcctgcagt tgctccgacc ggaactccca tccgttcata atcggcatca
     9301 tcaggtcgag caggatcacg cacgggcgct cctcgcgcgc gcgctcgagt gcaatctccc
     9361 cgttctcggc gggccgggac gtgtatccgg cgtcttcgag caacgtgcag atgctctgcc
     9421 gcagttgcgc atcgtcctcg acaaccagca cgcgagatgc cacttcgcga ctgcggattg
     9481 caagacttgt gcacgtcgaa aggccggtgc cctcgcggat cccgccgcgg tgcggggcga
     9541 aggtggattg gaagctggtg agccgcgtag gactcgaacc tacaacctag agattaagag
     9601 tctcttgctc taccaattga gctagcggcc caccgggacg cggaagaata gacgatcggg
     9661 tgcgagccgg caagcgttcg cagagggcct ctggattcgc gagcgagagc gacgactagc
     9721 cgcctccgac gaactcgacg acttcgagcg cgtccccttc caagagatgc gtggacgagt
     9781 acgccgattt cggaacgatc tcccggttgc gctcaaccgc tacgcgcggc gtcgagactc
     9841 cgagagcgac gagcagcgcg gcgatggagg ttccgtcggg aaaccttcgc tcgttaccgt
     9901 tcacgcgcac tcggacttcc atttcggctc ccgacgaacg acctccgtcg gtcatacgcc
     9961 cctccgtacc ggatactgga tttcggtcga acccgccgcg ga
//
LOCUS       DASWAV010000288         5233 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U2665
            k141_615681, whole genome shotgun sequence.
ACCESSION   DASWAV010000288 DASWAV010000000
VERSION     DASWAV010000288.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960316
            Sequence Read Archive: SRR10260007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 5233)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5233)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:47:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,929
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5233
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_615681"
                     /isolate="SMAG_U2665"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.36444 N 132.31889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            53..523
                     /locus_tag="VF958_05940"
     CDS             53..523
                     /locus_tag="VF958_05940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014216857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HEX9148687.1"
                     /translation="MARFTLSVNGKPRSVSVDPDTPLLWVLRDELDLTGTKYGCGEGL
                     CGACTVHVDGAAMRSCVVPVSSVARQKITTIEGLSAKGDHPVHRAWIEEDTPQCGYCQ
                     PGQIMCAASLLAFRPNPTDAEIDDAMSGNICRCGTYPRIRKAIHRAAALAKRKA"
     gene            520..2742
                     /locus_tag="VF958_05945"
     CDS             520..2742
                     /locus_tag="VF958_05945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012843097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin cofactor-binding domain-containing
                     protein"
                     /protein_id="HEX9148688.1"
                     /translation="MSPVPAFDRRTFLKVGATAAGGLLVGVWRVRASPESAAGGRPLA
                     LGAFVEIKPDGTVVVFSPRPEVGSGVKTSLAMLIAEELGVDWKNVTVRQPEMLDPKYL
                     DQFTGGSAGISDSWDPLRKAGAAAREVLVAAAAEKWQVPPGECRSENGAVLHPASGRR
                     LDYRELVERAAELKPPESPALKSTNEFRIAEKATATVDLAEIVTGRITYGLDVRLPGM
                     LHAVVERSPVFGGKVAKFDASRALSVPGVRKVVELPADRFALKSGRPIMVANGVAVIA
                     TSTWAAMKGRKALRVEWDEGPHAAESSAALRARLEKLASGTPGKILRDDGNVDTALAS
                     AAKTLEAVYEVPFVEAAPLEPVNCTARVKDGACELWAPTQNPYNARSFAATALGLQED
                     SWNSAITVHMVRPGGGFGRRLDTDYAAEAAYLAAQVPGEAVQVMSTRGDEIAHSLYRS
                     GGYHRMRAGLDASGSLVAWFHHLANCSRYRYQHGESPPWDSEISQDDFPAGHVANFRV
                     GYSEAESPIPRGYYRSMVPGATNFALECFLDEIARAAGRDPLAFRLALIGEGRVILYA
                     NYGGPQLDTARLRRVLELAAAKGDWGRPVAKGRGRGIAGGFVFGSYSAAVAEVSIARD
                     RSVRVHRVVSAVDCGRVINPSGAAKQIEGAVNDALNLALKLEITFDKGRVVQKNFTDY
                     PPVRMRESPKHIESHLVASDKPPTGLGEIPVPPAISAIANAVSAITGKRVRKLPIRLA
                     "
     gene            complement(2739..3011)
                     /locus_tag="VF958_05950"
     CDS             complement(2739..3011)
                     /locus_tag="VF958_05950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9148689.1"
                     /translation="MDLLWKVQVRGSAGKPFKYRVTFMWDEAGARLTPLVAANLYAEG
                     DLAVLLEDFGADRGDISSLLDKLEPARHAEIVVRPSEELLEVLWAS"
     gene            complement(3178..>5233)
                     /gene="lon"
                     /locus_tag="VF958_05955"
     CDS             complement(3178..>5233)
                     /gene="lon"
                     /locus_tag="VF958_05955"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015248981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HEX9148690.1"
                     /translation="QKLGLPEETVNQVLAEVDAPGRLADLVAGYIDIPPAERQALLET
                     LAVEDRLRRVLVHVQRQTDVLSAQEDIQEKVKEELGGRQREMYLREQLKAIQKELGEG
                     EGADDESLKELRAKLDALDLPEDARKEVDREWARLTRIGRESMESQVIRTYLETIAEL
                     PWNTRSEEHLDVAEAARILDEDHYGLKDVKDRILEFLAVRQLTQATEKAMETQAAAET
                     EPTPEPGAPKDALRKSARARGRILLFVGPPGVGKTSIAKSIARAMGRKYVRISLGGAR
                     DEADVRGHRRTYIGAMPGRIIQGMKQAGTKNPVFLLDEVDKLGVSFQGDPASALLEVL
                     DPAQNDSFTDHYLAVPFDLSEVLFIATANFIQNIPGPLLDRMETVNFAGYTEEEKLQI
                     ARSYLVPRQLEENGLSAGQLTIEDSAILEVVASYTRESGVRQLERELGKLARKVARRV
                     AAGEVQSLTVRQDEIRALLGRPRVHPEKMAHEDQIGTATGMYYTPAGGDIMFVEASLM
                     RGKGELILTGQLGDVMKESARAAWSFARSHAGWLGIEENEFVRDVHVHVPAGAIPKEG
                     PSAGVAIASALVSALSKRPVRNDVAMTGEITLSGRVLPIGGLKEKILGAVRAGITTVV
                     IPKANGPDLDDLTEEVRSKIEVHPVETLGEALAVTLRDTSLEEGRLLFGTSRDQPGTR
                     PQ"
BASE COUNT          792 a         1833 c         1748 g          860 t
ORIGIN      
        1 gggacgacct cgtcaacttt tcggagccgc ctccgtagga tcggggagcg gcatggcgag
       61 gttcactctt tcggtcaacg ggaagccgcg atcggtctcg gtcgatcccg acacgccgct
      121 tctgtgggtg ctgcgcgacg agctcgacct caccgggacg aagtacggct gcggagaggg
      181 gctctgcggc gcgtgcacgg ttcacgtcga cggagccgcg atgcgttcct gcgtcgtccc
      241 cgtctcctcc gtcgcccgcc agaagatcac gacgatcgag ggtctctccg cgaaggggga
      301 tcatcccgtc catcgagcct ggatcgagga ggacacgccc cagtgcggct actgccagcc
      361 gggccagatc atgtgcgccg catcgctgct cgcgtttcgt ccgaatccga ccgacgccga
      421 gatcgacgac gcgatgtcgg gcaacatctg ccgctgcggg acgtatccgc gcatccggaa
      481 ggcgatccac cgcgcggcgg ccctcgcgaa acggaaggca tgagtccagt cccggccttc
      541 gaccgccgca cgttcctcaa ggtcggcgcg actgccgccg gcggactcct cgtcggcgtc
      601 tggcgcgtca gggcgtcgcc cgagagcgcg gccgggggcc ggccgctcgc cctcggcgcg
      661 ttcgtcgaga tcaagcccga cgggacggtg gtcgtcttct ctccgcgtcc cgaggtcgga
      721 tcgggggtca agacgtcgct cgcgatgctc atcgccgaag agctcggcgt cgactggaag
      781 aacgtgacgg tccgccagcc cgagatgctc gaccccaagt acctcgatca gttcaccggc
      841 ggcagcgccg ggatctccga ctcgtgggac ccgctccgga aggccggcgc cgcggcgcgc
      901 gaggtcctcg tcgcggccgc cgcggagaag tggcaggtcc cgccagggga gtgccggtcg
      961 gagaacggcg ccgtcctcca tccggcgagc ggtcgccggc tcgactatcg cgagctcgtc
     1021 gaacgcgccg ccgagctgaa gccgccggag agcccggcgc tcaagtcgac gaacgagttc
     1081 cgcatcgcgg agaaggcgac ggcgacggtc gacctcgccg aaatcgtcac cggacgcatc
     1141 acgtacggcc tcgacgtgcg cctccccgga atgctccacg cggtcgtcga gcgcagcccc
     1201 gtgttcggag ggaaggtcgc caagttcgac gcttcacgcg cgctctcggt tcccggcgtc
     1261 cggaaggtcg tcgagctccc ggcggatcgc ttcgcgctca agtccggacg cccgatcatg
     1321 gtcgccaacg gcgtcgccgt catcgcgacg tccacgtggg ctgcgatgaa ggggcggaag
     1381 gccctgcgag tggagtggga cgagggccct cacgcggcag agagctcggc ggccctgcgc
     1441 gcgcggctcg agaagctggc ttctggaacg ccggggaaga tcctccgcga cgacggcaac
     1501 gtcgacacgg cgctcgcctc tgcggcgaag acgctcgagg ccgtttacga ggttcctttc
     1561 gtcgaggcgg cgcctctgga gcccgtcaac tgcaccgcgc gcgtgaaaga cggcgcgtgc
     1621 gagctgtggg cgccgaccca gaatccctac aacgctcgca gcttcgcggc aacggcgctc
     1681 ggtctgcagg aagactcctg gaactcggcg atcaccgttc acatggtccg cccgggcgga
     1741 ggattcggtc gccgcctcga cacggactac gcggcggagg ccgcatacct cgctgcccag
     1801 gtccctggcg aagccgtcca ggtgatgtcg acacggggcg acgagatcgc gcacagcctc
     1861 taccgatcgg gcggctatca ccggatgcgc gcagggctcg acgcgtcggg atcgctcgtc
     1921 gcctggttcc accacctcgc gaactgctcc cggtaccgct accagcacgg cgagtccccg
     1981 ccgtgggatt ccgagatctc ccaggacgac ttccccgcag gccacgtcgc gaacttccgc
     2041 gtcgggtaca gcgaggccga gagcccgatt ccgcgcggct actaccgctc gatggttccg
     2101 ggcgcgacca atttcgcgct tgagtgtttc ctggacgaga tcgcgcgcgc cgccggaagg
     2161 gacccgctcg cgtttcgcct cgcgttgatc ggagaaggac gggtgattct ctacgcgaac
     2221 tatggcggcc cgcagctcga cacggcgcgc ctgcgtcgcg tcctcgagct cgccgccgcg
     2281 aagggggact ggggaagacc ggtggcgaaa ggacgcggcc gcggaatcgc cggggggttc
     2341 gtcttcggtt cctactcggc ggcggtggcg gaagtctcga ttgcgcgcga tcgcagcgtg
     2401 cgcgtccacc gcgtcgtctc ggcggtcgac tgcgggcgag tgatcaatcc ctccggtgcg
     2461 gcgaagcaga tcgagggcgc ggtcaacgac gcgttgaacc tcgcgctcaa gctcgagatc
     2521 acgttcgaca aggggcgcgt cgtccagaag aacttcacgg actatccgcc ggtgcggatg
     2581 cgcgagtcac caaagcacat cgagagtcac ctggtcgcga gtgacaagcc tccgaccggg
     2641 ctgggcgaga taccggtccc gccggcgatc tccgcgatcg caaacgcggt ctcggccatc
     2701 acggggaaac gcgtccgcaa gcttccgatc cggctcgcct aactcgccca gagcacctcg
     2761 aggagctctt cggacggtcg gacgacgatc tccgcgtgcc gcgcaggctc cagcttgtcg
     2821 aggaggctgg aaatatctcc ccgatccgcg ccgaaatctt cgagcaagac cgcgaggtcg
     2881 ccttccgcgt agaggttcgc cgcgacgagc ggggtcaggc gcgctccggc ctcgtcccac
     2941 atgaacgtga cgcggtactt gaacggcttg cccgccgaac cccgcacctg caccttccag
     3001 agaagatcca tgaaggctcc cttcatccgg aatctgacgc ggaggtccgg aattctcgca
     3061 atgcttgtcg ttcgggattc gtcaggaccg gctggcgaag ggcgagcgaa ccgagaacga
     3121 aatcagtcta tcactcgcga cgaccgccac cccgcgagaa ccgtgcgcag cggccgccta
     3181 ctgcggccgg gtcccgggct ggtcgcggct cgtgccgaac agcagccgcc cctcttccaa
     3241 cgacgtgtcg cgcagcgtga ccgcgagcgc ttcccccagc gtctcgaccg ggtgcacctc
     3301 gatcttcgac cgcacctctt cggtcaagtc gtccagatcc ggcccgttcg ccttcgggat
     3361 cacgaccgtc gtgatgcccg cgcgcaccgc gcccaggatc ttctccttca ggccgccgat
     3421 cgggagaacc cgccccgaga gcgtgatctc gccggtcatc gcgacgtcgt ttctcaccgg
     3481 gcgcttcgag agcgccgaca cgagggccga tgcgatcgcg acaccagcgg acggtccctc
     3541 cttcgggatc gcacccgccg ggacgtgcac gtgcacgtcg cgtacgaatt cgttctcttc
     3601 gatgccgagc cagcccgcgt gggagcgcgc gaacgaccat gctgcacggg ccgattcctt
     3661 catgacgtcg ccgagctgtc cggtcaggat cagctcgccc ttgccgcgca tgagcgacgc
     3721 ttcgacgaac atgatgtcgc cgccggcggg cgtgtagtac atccccgtcg cggtgccgat
     3781 ctggtcctcg tgcgccatct tctccgggtg gacgcgcggc cggccgagga gggcccggat
     3841 ctcgtcctgc cggaccgtga ggctctgcac ttcgcccgcg gcgacgcgcc gcgcgacttt
     3901 gcgggcgagc ttcccgagct cgcgctcgag ctgccggacg ccggactcac gcgtgtagct
     3961 cgccacgacc tccaggatcg cgctgtcctc gatcgtgagc tggccggccg agagaccgtt
     4021 ctcctcgagc tgccgcggca cgaggtagct cctcgcgatc tgcagcttct cttcctcggt
     4081 gtaacccgcg aagttgaccg tctccatacg gtcgagcagc gggcccggaa tgttctggat
     4141 gaagttcgcc gtcgcaatga acagaacttc cgaaaggtcg aagggtacgg cgagatagtg
     4201 gtccgtgaat gagtcgttct gcgcggggtc gagcacctcg aggagtgccg acgccggatc
     4261 gccctggaac gacacgccga gcttgtcgac ctcgtcgaga aggaagaccg ggttcttcgt
     4321 gcccgcctgc ttcatcccct ggatgatccg acccggcatc gctccgatgt acgtgcgccg
     4381 gtggccgcgc acgtccgctt cgtcgcgggc tccgccgagt gagatgcgga cgtacttgcg
     4441 ccccatggcg cgggcgatcg acttcgcgat cgacgtcttg ccgacgcccg gcggcccgac
     4501 gaagagcagg atgcgtcccc gcgcccgcgc cgacttgcgc aacgcgtcct tgggagcgcc
     4561 cggctccggt gtcggctcgg tctcggcggc cgcctgcgtt tccatcgcct tttcggtcgc
     4621 ctgcgtgagc tgccggaccg cgaggaactc gaggatccgg tccttgacgt ccttcagccc
     4681 gtagtggtcc tcgtcgagga tgcgcgccgc ctctgcgacg tccaggtgct cctcgctcct
     4741 cgtgttccag ggtagctccg cgatcgtctc gaggtaggtc cggatgacct gtgactccat
     4801 ggactcgcgg ccgatgcgcg tcaggcgcgc ccattcgcgg tcgacctcct tgcgcgcgtc
     4861 ctccggcagg tcgagcgcgt cgagcttggc gcgaagctcc ttcaacgact cgtcgtccgc
     4921 accttcgccc tcgccgagtt ccttctgaat cgccttcagc tgctcgcgca gatacatctc
     4981 gcgctgccgg ccgccgagct cctccttgac cttctcctgg atgtcttcct gagcggagag
     5041 gacgtcggtc tgccgctgca cgtgaacgag cacgcggcgc aggcggtctt cgaccgccag
     5101 ggtctcgagg agcgcctggc gctcggccgg cggaatgtcg atgtagccgg cgaccaggtc
     5161 cgcgaggcgc ccgggggcgt cgacttccgc aagcacctgg ttcaccgtct cctcgggaag
     5221 cccgagcttc tgg
//
LOCUS       DASWAV010000289         8558 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U2665
            k141_495646, whole genome shotgun sequence.
ACCESSION   DASWAV010000289 DASWAV010000000
VERSION     DASWAV010000289.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960316
            Sequence Read Archive: SRR10260007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 8558)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8558)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:47:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,929
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8558
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_495646"
                     /isolate="SMAG_U2665"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.36444 N 132.31889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..515)
                     /locus_tag="VF958_05960"
     CDS             complement(<1..515)
                     /locus_tag="VF958_05960"
                     /inference="COORDINATES: protein motif:HMM:NF012584.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoyl-containing protein"
                     /protein_id="HEX9148691.1"
                     /translation="MNVRRRFVWRRKSGHVDVSVESRDGRLVTFRIGDQETTADCAKL
                     PGGRSTVILPSGRQLTGRAAGRRDGRIEAWNGARRVHLELADPLANLVAESANAAGGA
                     TEIRAQIPGRVVEVRVSAGDQVAAGTTLLVLEAMKMQNEIRAEAAALVVRVDCVPGQT
                     VETGFLLVRLES"
     gene            complement(512..2011)
                     /locus_tag="VF958_05965"
     CDS             complement(512..2011)
                     /locus_tag="VF958_05965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011683622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin carboxylase N-terminal domain-containing
                     protein"
                     /protein_id="HEX9148692.1"
                     /translation="MRLLVANRGEIAVRIFRACRDLGIETVAVYSDADRAALHVTMAD
                     YAVRLGPAPSSESYLSIPRILDAAKKTGASLVHPGYGFLAESADFARASADAGLTFVG
                     PSASAIDAMGAKTTSRELMHAAGVPIVPGTTTPARDVAELAVFALGSGFPILLKASAG
                     GGGKGMRRVDSEGDIQEAFERASAEAQAAFGDGSIYAEKLLERPRHVEVQIAADQRGH
                     RVAVGERECSLQRRHQKVVEECPSPVVTPELRSRLFAAALAAADAVAYDSCGTVEFLL
                     AADGSFYFLEMNTRLQVEHPVTEEVWGVDLAREMIRAALGEPLSFRADDLAPRGHAIE
                     CRVYAEDAARGFAPSPGVVNRLRLPEGPGVRNDVGIEEGSAVPIDYDPMLGKLIASGQ
                     DRGAAIARLARALDEYEIAGVDTTVPLFRALVSDPDFRAARFHTQWLDQWLAGQSLDP
                     EEATVEEAVLAAASISIDGGTPAPARSDRGSRWREAGRVEALRTSRLRS"
     gene            complement(2042..3583)
                     /locus_tag="VF958_05970"
     CDS             complement(2042..3583)
                     /locus_tag="VF958_05970"
                     /EC_number="6.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006929980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA carboxylase subunit beta"
                     /protein_id="HEX9148693.1"
                     /translation="MTIEKRIADLEALRERSLQGGGADRVARQHAEGKLTARERVELL
                     LDPGSFFELDRLVEHRSRDFGMDRTRIPGDGIVSGAGRVDGRPVYVFAQDFTVFGGSL
                     SETASEKICKVMDLAVVNGAPIIGLNDSGGARIQEGVVSLAAYSRVFLRNTRASGVVP
                     QISAILGPCAGGAVYSPAITDFVFMTEKTSYLFVTGPDVIKTVTHEEVTKEDLGGART
                     HSSVSGVAHFVRRDDAGVLFGIRELLSYIPSNNLDDPPMRESREPIAGSRDLDRLIPE
                     EPDRSYDMIDLIRAVADGGEFFEVHASFARNLIVGFARLGGRSVGVVANQPMHLAGVL
                     DIDASVKGARFVRFCDAFNIPLLVFEDVPGFLPGTQQEYGGIIRHGAKLLYAFAEATV
                     PKVSVITRKAYGGAYCVMLSKDIATDFNFAFPTAEVAVMGPQGAVNILYRREFESAPP
                     EIRAERVSEYVENFANPYVAARRGYIDEIIEPSETREKLIAAFALLEGKRASMPSKKH
                     GNIPL"
     gene            complement(3580..3858)
                     /locus_tag="VF958_05975"
     CDS             complement(3580..3858)
                     /locus_tag="VF958_05975"
                     /inference="COORDINATES: protein motif:HMM:NF016271.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF507 family protein"
                     /protein_id="HEX9148694.1"
                     /translation="MSLADRKKEFVAVRTARGLTTRKLFEEKAPGALREAVLSALAAE
                     AEKERTLDEEARRMMEAARHEIASGKVDGQELFRRIRRKLAEQRGIVL"
     gene            3947..5242
                     /locus_tag="VF958_05980"
     CDS             3947..5242
                     /locus_tag="VF958_05980"
                     /inference="COORDINATES: protein motif:HMM:NF039907.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallopeptidase TldD-related protein"
                     /protein_id="HEX9148695.1"
                     /translation="MARWLDPLVIGRGDIADVFIEKRQRTVVEWRDGQAAEPRVELEA
                     GTSARLKRSDREKLAFVSGADETAIREAIRGLQASAGRTPLPARTAPPRSEIPRGSSA
                     ERWVRRLPAILARHAPHHRLRWTLDEVTRRVLPSGLPSAGSERRFFSLEGTFVAASRR
                     GDETRSFSFHAPEADATADELKEALTAAAAPRERPVAFGDGNADVLFSGGTAAILFHE
                     ILSHALEADAEESPLSALAGARLSVADLEVRDDPTRLDLFGGYERDDEGARPRPVKLL
                     DAGRLGGKLTDRARGGTRGSTGHGRRAGAADVPLPRGSNTVVSPGAATTEEMTRRLGN
                     GIWIDEIDAGTVELSSGQFRIRYPGGRRVRRGRFADEIGPGLLAGEILSALSGVEPVI
                     GREARACRSLAWCSRAGQIVPVGGAAPDVIVRRLSLRDR"
     gene            5239..6489
                     /locus_tag="VF958_05985"
     CDS             5239..6489
                     /locus_tag="VF958_05985"
                     /inference="COORDINATES: protein motif:HMM:NF039907.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallopeptidase TldD-related protein"
                     /protein_id="HEX9148696.1"
                     /translation="MKGPELPALLERHAPGAWELYRKTAETQEADVSAHNRTELSRRE
                     EGLAARWWAGGLRFACGSDPGRLADAIGKASEFPASGPAEVRLPSRGAVRPVSEAAVD
                     PPPDLFARLSQLVGEKSRGQATLASLALRRGRAVEEMRNSAGLSVRMESVRLDGVARA
                     VGRHGSRACESRVVFRWDAEPDLDAIAARLADGATFPLAPRGLSFPRGSLLLDPSVAA
                     ALLAAIAPLFSTHPLPRWVKRSELASREVTIADDATADAPFDGEGTPTRRVVVVEDGA
                     FTSRLHDISSAAAAGEPPTGHGVRPSYRTPPRAAGRRLFFETARGVAPLELLRRVARG
                     VFARAVTAPLSVDLTRDRYEIEFTGVAVLAGRATEPVAGARVAGRLSELLQRIAAVAT
                     DRQFFPMPYPVGAPTVLVEKVSFE"
     gene            complement(6594..7442)
                     /locus_tag="VF958_05990"
     CDS             complement(6594..7442)
                     /locus_tag="VF958_05990"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX9148697.1"
                     /translation="MKRFSIAMLALAAAVTVGCGLLHAKRQTSGEPFYRKYLVAGNAL
                     DDRIAEQERLVAAQPDSADLRNDFGNLLAERRFVEDAHMQYEKALELDRTNYVAAYNL
                     GLLWEVAGKSGRAIGAYRKSISRKPGFPASHFHLGRLYEQRGWDGLAVKEYAKALRLD
                     PQMRDPRSNPLVVDTRLLDRVSLVNYQHDLASAAMSSDRAWADEGRFRNVPTDRPLSS
                     SEVEDASALEPVDATVTAPAKHAPAPVEMPRQVGQTRPQQGLPPGTPPLLPAGAPPPP
                     APTPRP"
     gene            complement(7457..8050)
                     /locus_tag="VF958_05995"
     CDS             complement(7457..8050)
                     /locus_tag="VF958_05995"
                     /inference="COORDINATES: protein motif:HMM:NF041621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MXAN_5187 C-terminal domain-containing protein"
                     /protein_id="HEX9148698.1"
                     /translation="MTWDRELRVLGEAIRRLNAEYDAFLFGSVTRPPVQSRRHVEEMF
                     RRLTQSPSESAADRYQLSTLQGRFSTLCERWERLQAEKEAGRRPGIPAGFSTPEPGSS
                     PNAGKPGSVEANREDGSPRDRQLFERYVAARGRLGEEVSGFRLEQFLARLAEERGRLR
                     ERLGVAEIDFDVVERDGRVKLVAKPKESPKSEIRGRK"
     gene            8070..>8558
                     /locus_tag="VF958_06000"
     CDS             8070..>8558
                     /locus_tag="VF958_06000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006904803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-seryl-tRNA(Sec) selenium transferase"
                     /protein_id="HEX9148699.1"
                     /translation="MSAARPPSMDLLLARPEAAEPLGLYGREAVKEELRRAIAAGETE
                     PDALLALARASLLSRFAPTLVRAVNATGVLLHTNLGRSPSSEAAREAIARIASGYSTL
                     EYDAASGQRGKRQDHVRRAACDLFGAEDAIAVNNNAAAVFLALAALARGRAVLVSRGE
                     LVA"
BASE COUNT         1258 a         3022 c         2972 g         1306 t
ORIGIN      
        1 gactccaggc ggacgaggag aaatcccgtt tcgaccgtct gaccggggac gcagtcgacg
       61 cgaacgacga gcgcggccgc ctccgcccgg atttcgttct gcatcttcat ggcctccagg
      121 acgaggagag tcgtcccggc ggcgacctgg tcgcccgcgg agaccctcac ctccaccacc
      181 cgaccgggaa tctgcgcccg aatctcggtc gccccgccgg cggcattcgc cgactctgcg
      241 acgaggtttg cgagcgggtc ggccagctcg aggtgaacgc gccgggcgcc gttccaggct
      301 tcgatgcggc cgtcgcggcg cccggcggcc cgtccggtca gctggcggcc ggaggggagg
      361 atgacggtcg agcggccgcc gggaagcttc gcgcagtcgg ccgtggtctc ctggtctccg
      421 atccggaaag tgaccaggcg tccgtcccgc gactcgacgc tgacgtcgac gtggccgctc
      481 tttcgcctcc agacgaagcg ccggcggacg ttcacgaccg cagtctcgaa gttcgaagcg
      541 cctcgacgcg gccggcttct cgccagcggg atccgcggtc cgatcgcgcc ggcgccggcg
      601 taccgccgtc gatcgagatc gacgcggccg cgagaacggc ttcctcgacc gtcgcttcct
      661 ccggatccag actctgacct gcgagccatt ggtcgagcca ctgcgtgtgg aagcgcgcgg
      721 cacggaagtc cgggtcggag acgagcgcgc ggaagagagg aacggtcgta tcgactccgg
      781 cgatctcgta ttcgtcgaga gcgcgcgcga ggcgcgcgat cgccgcaccc cggtcctgcc
      841 cggacgcgat cagcttgccg agcatggggt cgtagtcgat cgggaccgcg gatccctctt
      901 cgatcccgac gtcgtttcgc acgccgggcc cctcgggcag ccggagacgg ttcacgactc
      961 cgggagaagg agcgaacccg cgggcggcgt cctcggcata gacgcggcac tcgatggcgt
     1021 ggccgcgcgg agcgagatcg tcggcgcgaa aagagagcgg ttccccgagc gctgcgcgga
     1081 tcatctctcg cgcgaggtcg acgccccaca cttcctcggt caccggatgc tcgacctgca
     1141 gccgcgtgtt catctcgaga aaatagaacg agccgtcggc cgcgagcagg aactcgacgg
     1201 tcccgcagga gtcgtaagcg acggcgtccg ccgccgcgag cgctgcggcg aagaggcggg
     1261 agcgaagctc cggggtgacg accggcgacg ggcactcctc gacgaccttc tggtggcgcc
     1321 gctggaggct gcactcacgc tctccgaccg cgacacggtg gccgcgttga tcggcggcga
     1381 tctgcacctc gacgtggcgc ggccgctcca ggagcttctc ggcgtaaatc gagccgtcgc
     1441 cgaacgcagc ctgcgcctcc gcggacgccc gctcgaacgc ctcctggatg tccccctccg
     1501 agtcgacccg gcgcatcccc ttgccgccgc cgcccgcgga cgccttcagg agaatcggaa
     1561 accccgagcc gagcgcgaaa accgcgagct cggccacgtc gcgggccggc gtcgtcgtgc
     1621 cggggacgat cggaacaccc gcggcgtgca tcagctcgcg gctcgtcgtc ttcgcgccca
     1681 ttgcgtcgat cgcggatgcc gacggtccca cgaacgtgag ccccgcgtcc gcgctggcgc
     1741 gcgcgaagtc ggcgctctcg gcgagaaagc cgtagccggg gtgaacgagc gacgcacccg
     1801 ttttcttcgc ggcgtccagg attcgcggga tcgagaggta gctctcggag gacggcgccg
     1861 gccccaggcg caccgcgtag tcggccatgg tgacgtggag agcggcgcga tcggcgtccg
     1921 aatagaccgc gaccgtctcg attccgaggt cccggcacgc ccggaagatg cggacggcga
     1981 tctcgccgcg gttcgcgacg aggagtctca tgcttcccct gtcattgacg atgggaagac
     2041 atcacagcgg gatattcccg tgcttcttcg agggcatgga cgcccgcttc ccttcgagca
     2101 acgcgaacgc ggcgatcagc ttttcccgcg tctcggacgg ctcgatgatc tcgtcgatgt
     2161 aaccccggcg cgcggcgacg taggggttcg cgaagttctc gacgtactcg gacactctct
     2221 ccgcgcggat ctcggggggc gcgctctcga actcgcggcg gtagaggatg ttcacggcgc
     2281 cctgcggccc catcaccgcg acctcggccg tgggaaacgc gaagttgaaa tcggtcgcga
     2341 tgtccttgga caacatgacg cagtacgcgc cgccgtatgc cttgcgcgtg atcaccgaga
     2401 ccttcggcac ggtcgcctcc gcgaacgcgt agagcagctt cgcgccgtgc cggatgatgc
     2461 cgccgtactc ctgctgagtc ccgggaagga acccgggaac gtcctcgaaa accaggagcg
     2521 gaatgttgaa cgcgtcgcaa aaccggacga agcgcgcgcc cttcaccgaa gcgtcgatat
     2581 cgagcacgcc ggcgagatgc atcggctggt tcgcaacgac tccgacggag cggccgccga
     2641 gccttgcgaa gccgacgatg aggttccgcg cgaaggaggc gtggacttcg aagaactctc
     2701 ctccgtccgc gaccgcgcgg atcaagtcga tcatgtcgta cgaccgatcc ggctcctcgg
     2761 gaatcagccg atcgaggtcg cggctcccgg cgatcggctc ccggctctcg cgcatcggcg
     2821 gatcgtccag gttgttcgag gggatgtagg agagaagttc tcgaatcccg aacaacactc
     2881 cggcatcgtc gcggcgaacg aagtgggcga cgcccgagac cgacgagtgc gtgcgcgcgc
     2941 cgcccaggtc ttccttcgtg acctcttcgt gcgtcacggt cttgatgacg tccgggccgg
     3001 tgacgaagag ataggaagtc ttctcggtca tgaagacgaa gtccgtgatc gcgggggaat
     3061 agacggcgcc gccggcgcag ggaccgagga tcgccgagat ctgaggcacc acgcccgacg
     3121 cgcgcgtgtt tcgaaggaaa acgcgagagt aggcggccag cgagaccacg ccctcctgga
     3181 tgcgggcgcc tcccgagtcg ttcaggccga tgatcggcgc tccgttgacg accgcgaggt
     3241 ccatgacctt gcagatcttc tcggaggcgg tctccgacag agatccgccg aacacggtga
     3301 agtcctgcgc gaagacgtag acgggacgcc cgtccaccct gccagcgccc gagacgatgc
     3361 cgtcgccggg gatgcgcgtg cggtccatcc cgaagtcgcg cgaccgatgc tcgaccagac
     3421 ggtcgagctc gaagaacgag ccggggtcga gaaggagctc gacccgctcg cgggctgtca
     3481 gcttcccctc cgcgtgctgc cgcgccaccc gatcggcgcc gccgccctgc aggctcctct
     3541 cccggagagc ctcgagatcc gcgatccgct tttcgatcgt cagagtacga ttccccgctg
     3601 ttcggcgagc ttgcggcgga tccgtcggaa gagctcctgg ccgtcgacct tccccgaagc
     3661 gatctcgtgc cgagccgctt ccatcatccg ccgcgcctct tcgtcgagag tgcgttcttt
     3721 ttccgcttcc gcggcgaggg ccgagaggac ggcctcgcgc agcgcgccgg gcgctttctc
     3781 ctcgaaaagc ttccgcgtcg tcaggccgcg cgccgtccga accgcgacga actctttctt
     3841 ccggtccgcc agactcatcc gtctctcccg cggcgcgatg ttacagcggg aaaaacgtcc
     3901 cacataatcg cgcggcgtgg agcactcgga atgggatgaa caagccgtgg cgcggtggct
     3961 cgacccgctc gtcatcggtc ggggagacat cgccgacgtc ttcatcgaga agcggcagcg
     4021 gaccgtcgtc gagtggcgcg acggccaggc cgcggagccc cgcgtcgagc tcgaagccgg
     4081 gacgtcggcg cggctcaagc ggtcggaccg ggagaagctc gccttcgtct cgggggcgga
     4141 cgaaacggcg atccgcgagg cgatccgcgg gctgcaggcg tccgccgggc gaacccccct
     4201 gcccgcccgg accgcgccgc cgcgttccga gatcccccgc ggctcctccg cagaacggtg
     4261 ggtccggcgc ctgccggcta tcctcgcgcg acacgctccg caccaccggc tgcgctggac
     4321 cctcgacgag gtgacgcggc gcgtgctccc gtcgggcctt ccttcggcgg gatcggaacg
     4381 ccgcttcttc tcgctcgagg gaacgttcgt cgcggcctcg cgccgtgggg acgagacgcg
     4441 ctccttctcg tttcacgcgc cggaggccga cgcgaccgcc gacgagctca aggaagcgct
     4501 cacggcggcc gccgcgcccc gcgagcggcc ggtcgcgttc ggggacggaa acgccgacgt
     4561 tctcttctcc ggcgggacgg cggcgattct ctttcacgag atcctctccc acgcgctcga
     4621 agcggacgcg gaagaatcgc cgctctccgc tctcgcgggt gcgcggctgt cggtcgcgga
     4681 cctcgaggtc cgggacgatc cgacgcggct cgatctcttc ggagggtatg agcgcgacga
     4741 cgaaggggcg cgtccgcgtc ccgtgaagct cctcgacgcg ggccggctcg gcgggaagct
     4801 caccgatcgc gcgcgagggg ggacgcgcgg ctcgaccgga cacggccggc gcgcgggcgc
     4861 ggccgacgtt ccgctgccgc gcggatcgaa cacagtcgtc tcccccggtg ccgcaacgac
     4921 cgaggagatg acgcggaggc tcggaaacgg gatctggatc gacgagatcg acgccggaac
     4981 ggtcgagctc tcgagcggac agttccggat ccgctatccg gggggccggc gcgtccggcg
     5041 gggccgcttc gccgacgaga tcgggcctgg tctgctcgcc ggagagattc tttcggccct
     5101 gtcgggcgtc gagccggtga tcggccgcga ggcgcgggcc tgccgctcgc tcgcctggtg
     5161 ctcgagggcc ggccagatcg tcccggtcgg cggcgccgcg cccgacgtga tcgtgcgccg
     5221 gctctctctg cgagatcgat gaagggcccc gagctgcccg cgcttctcga gcggcacgcg
     5281 ccgggcgcgt gggagctcta tcgaaagacg gccgagacgc aggaggcgga cgtctccgca
     5341 cacaaccgca cggagctctc gcgccgggag gaaggcctgg ccgcgcgatg gtgggcgggc
     5401 ggcctccgat tcgcctgcgg ttcggacccc gggaggctcg cggacgcgat cggcaaggcg
     5461 tcggagtttc ccgcgagcgg cccggcagag gtccgactgc cgtcgcgcgg ggccgtgcgg
     5521 ccggtctcgg aggcagccgt ggatccgccg cccgatcttt tcgcgagact gtcgcagctg
     5581 gtgggcgaga agtcgcgcgg gcaggcgacg ctcgcgtcgc tcgcgcttcg ccgcggacgg
     5641 gcggtcgaag agatgcgcaa ctccgcggga ctttccgtga ggatggagtc ggtccgcttg
     5701 gacggcgtcg ctcgcgcggt cggccggcac ggctcgcggg cctgcgagtc gcgggtcgtg
     5761 ttccgctggg acgccgagcc ggatctcgat gcgatcgcgg cgcggctcgc cgacggggcg
     5821 acgttccccc tggcgccgcg cggcctctcc ttcccgcgcg gctccctgct cctcgaccct
     5881 tcggtggccg ccgcactcct cgcggcgatc gcgccgctct tctcgaccca tccccttccg
     5941 cgctgggtca agcggagcga gctcgcttcg cgcgaggtca ccatcgcgga cgacgcgacc
     6001 gcggacgccc cgttcgacgg cgagggcact ccgacgcggc gtgtcgtcgt cgtcgaagac
     6061 ggagcgttca cgtcgcgcct ccacgacatc tcttccgccg ccgccgccgg cgagccgccg
     6121 acgggacacg gcgtgcgccc gtcctatcgc acgcctcccc gcgccgcggg aagacgtctg
     6181 ttcttcgaga ccgcgcgcgg cgtcgcgccc ctcgagctcc tccgccgggt cgcgcgaggc
     6241 gtcttcgccc gcgcggtgac ggcgcctctc tccgtggatc tcacgcgcga ccgctacgaa
     6301 atcgagttca cgggcgtcgc ggtgctcgcg ggacgagcca cggagccggt cgcgggcgca
     6361 cgcgtagccg ggcggttgtc ggagcttctc cagcgcatcg ccgccgtcgc gaccgaccgg
     6421 cagttctttc cgatgcccta tcccgtgggg gcgcccacgg ttctcgtcga gaaggtcagc
     6481 ttcgagtgag cgaggtcagg tcttcacgga tatggaaggc acccgccagg agagagtgac
     6541 cttgccatct ttgcgaagca aagtggcaag tggagacctg acccccggct cccttacggg
     6601 cgaggtgttg gcgccggggg cggcggcgcg cccgcgggca ggagcggcgg cgttcccgga
     6661 ggcagcccct gctgcggccg ggtctgaccg acctgacgcg gcatctccac cggcgcaggc
     6721 gcgtgcttcg caggcgccgt gaccgtggcg tcgacaggct cgagcgccga ggcgtcttcc
     6781 acctcggagg acgacagcgg gcggtccgtc ggcacgttcc ggaaacgtcc ctcgtccgcc
     6841 cacgcccgat ccgagctcat cgccgcggac gcgagatcgt gctgatagtt cacgagcgat
     6901 acccggtcga ggagacgcgt gtcgacgacg agcgggttcg accgcgggtc tctcatctgc
     6961 gggtcgagtc ggagcgcctt cgcgtactcc ttcaccgcga gcccgtccca accccgttgc
     7021 tcgtagagac ggccgaggtg aaagtgcgag gccggaaacc ccggcttcct cgagatcgac
     7081 ttgcgatagg cgccgatcgc acggccgctc ttgccggcca cttcccagag cagcccgagg
     7141 ttgtaagcgg cgacgtagtt tgttcgatcg agctcgagcg ccttctcgta ctgcatatgc
     7201 gcatcctcca cgaaccggcg ctcggcgagg aggtttccga agtcgttgcg caggtcggcc
     7261 gagtccggct gcgccgcgac gaggcgctcc tgttcggcga tccggtcgtc gagcgcattt
     7321 ccagcgacga gatactttcg atagaacggc tcgcccgacg tctggcgctt cgcgtggaga
     7381 agaccgcagc cgacggtcac ggcggcagcc agagcaagca tcgcgatcga gaacctcttc
     7441 attgaattcc tctcgtctac ttccggcctc ggatttcgga cttcggactc tccttcggtt
     7501 tcgcgacgag cttgaccctt ccgtcccgtt cgacgacgtc gaaatcgatc tccgcgaccc
     7561 ccagacgttc tctcaaccgg ccgcgttcct ccgcgaggcg cgcaagaaac tgctcgagcc
     7621 ggaagccgga gacctcttca ccgagcctcc cccgggcggc gacgtagcgc tcgaagagct
     7681 ggcggtcgcg cggcgacccg tcttcccgat tcgcttctac ggagccgggc ttgcccgcgt
     7741 tgggggaaga ccccggctcg ggggtcgaaa aaccggcggg gattccggga cggcgccccg
     7801 cctccttctc cgcttgcagc ctctcccagc gctcgcaaag ggttgagaaa cggccctgga
     7861 gggtcgagag ctggtagcgg tcggcggcgg attcggatgg ggactgggtc agccgccgaa
     7921 acatctcctc gacgtgccgc cggctctgga cgggcggtct cgtcaccgag ccgaagagga
     7981 aggcgtcgta ctcggcgttc aaccgccgga tcgcctcgcc gagcacccgg agctcgcggt
     8041 cccaggtcac gttctcgata atagcgcccg tgagcgccgc ccgaccccct tcgatggacc
     8101 tcctgctcgc gcgccccgag gccgccgagc ctctcggtct ttacgggcgg gaggcggtca
     8161 aggaggagct gcgccgcgcc attgccgcgg gcgagaccga gccggacgcg ctgctggccc
     8221 tcgcccgcgc ctctctcctc tcccgtttcg cgccgactct cgtccgggcg gtgaacgcca
     8281 ccggggtcct gctgcacacg aatctcggac gctccccctc gagcgaggcc gcccgcgagg
     8341 cgatcgcgcg catcgcgtcc ggctactcca cgctcgagta cgacgccgca agcggacagc
     8401 gcggcaagag gcaggatcac gtccggcggg cggcgtgcga cctgttcggc gcagaggacg
     8461 cgatcgccgt aaacaacaac gcggcggcgg tcttcctcgc actcgcggcg ctcgcccgtg
     8521 ggcgcgcggt cctggtctcc cgcggggagc tcgtcgcg
//
LOCUS       DASWAV010000290         2191 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U2665
            k141_535728, whole genome shotgun sequence.
ACCESSION   DASWAV010000290 DASWAV010000000
VERSION     DASWAV010000290.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960316
            Sequence Read Archive: SRR10260007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2191)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2191)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:47:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,929
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2191
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_535728"
                     /isolate="SMAG_U2665"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.36444 N 132.31889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..673
                     /gene="mreB"
                     /locus_tag="VF958_06005"
     CDS             <1..673
                     /gene="mreB"
                     /locus_tag="VF958_06005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013579277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="rod shape-determining protein MreB"
                     /protein_id="HEX9148700.1"
                     /translation="KRAVQDSALRAGASEVFIIEQAMAAAIGAGLPITEPAGNMIVDI
                     GGGTTDVAVISLAGIVYSRSVRIASNEMDEAIIQYVKRKYNLLIGERTAEQIKIEIGS
                     AYPLDEPLTMEVKGRDLVEGVPKTLTLTDEEVREALAEVVAAIVETVRVALERTPPEL
                     SADIMDEGIIITGGGSLLRNLDRRLREETGLPISYAEDPLASVALGTGAMLTDMNLLR
                     KISIT"
     gene            774..1640
                     /gene="mreC"
                     /locus_tag="VF958_06010"
     CDS             774..1640
                     /gene="mreC"
                     /locus_tag="VF958_06010"
                     /inference="COORDINATES: protein motif:HMM:NF016011.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005618 - cell wall [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HEX9148701.1"
                     /translation="MSARAEITPRPRRGGVLLASVLVLCVLALSAQAPARGRAGSVLQ
                     AWILTAAAPVVRAVAGVSRTVSGASSSAAETWTAREENTRLRRDLAKRDEELFRLRAE
                     VRQFRSESALRASAPFLPVVVGSAPVLLVERRAGLQSALVGIGSAAGIGGGAPLAVPE
                     GLVGRILISGRTISRAQLLLDASSAAGARIVRTGELGIVRGDGRGALRLNHIPTTSRV
                     QRGDLIETAGIDGVYPRGIPIGRVASVSPGGNLFLDILVVPTAPFSRLTDVLVLAPFT
                     ARVPADGTSVAR"
     gene            1637..2140
                     /gene="mreD"
                     /locus_tag="VF958_06015"
     CDS             1637..2140
                     /gene="mreD"
                     /locus_tag="VF958_06015"
                     /inference="COORDINATES: protein motif:HMM:TIGR03426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HEX9148702.1"
                     /translation="MKFGRIALIFAGAAALEFFFGGSKYRNAWPVDWLLVATAVVARS
                     GGFGRAVLTGAAAGLLEDALTESLLGMNAFAKAALGYVLAFVSVRVIFGGAIAVGGVI
                     ALCSLANDAVVAVLRALLLDSPIVLGAPYSLWRAAATGVTAGALEAAWTFPWREWRER
                     RRLRRLR"
BASE COUNT          316 a          725 c          786 g          364 t
ORIGIN      
        1 aaagcgcgcg gtccaggact cggcgttgcg cgccggcgcc tcggaggtgt tcatcatcga
       61 gcaggcgatg gcagcggcga tcggggcggg gcttcccatc accgagcccg ccggaaacat
      121 gatcgtggac atcggcggcg gaacgaccga cgtcgcggtc atctcgctcg cggggatcgt
      181 ctacagccgc tccgtgcgga tcgcttcgaa cgaaatggac gaggcgatca tccaatacgt
      241 gaaacgcaag tacaacctcc tgatcggtga acgcaccgcc gagcagataa agatcgagat
      301 cggctcggcc tacccgctcg atgagccact cacgatggaa gtgaagggac gcgacctcgt
      361 ggagggcgtt ccgaagacgc tgacgctgac cgacgaagag gtccgcgagg cgctcgccga
      421 agtcgtcgcg gcgatcgtcg aaaccgtgcg ggtcgcgctc gagcgcacgc cacccgagct
      481 ctccgccgac atcatggacg agggaatcat catcacgggc ggcgggtcgc tgctgcgcaa
      541 tctcgaccgg cgcctgcgcg aggagacggg cctgccgatc tcctacgccg aagatccgct
      601 tgcgtcggtc gcccttggca ccggcgcgat gctcaccgac atgaacctgt tgaggaaaat
      661 atccatcacg tgacgaaggc cggagatcgg aaatcggtag tgggatttcg agccggaaaa
      721 cggagctcct cgattcacga ttttcgattc tcgaccccgg accgatgagg cccttgagcg
      781 cgcgcgccga gatcaccccg cgcccgcgcc gaggcggggt ccttctcgcc tccgtcctcg
      841 tcctgtgcgt cctcgcgctc tcggcgcaag cgccggcccg cgggcgcgcg ggctcggttc
      901 tccaggcgtg gattctgacc gccgctgctc cggtcgttcg cgccgttgcc ggcgtgtcgc
      961 ggacggtttc gggcgcgtcg tcgtccgccg ccgagacgtg gaccgcgcgc gaagagaaca
     1021 cgcggctgcg ccgggacctc gcgaagagag acgaggagct cttccgcctc cgcgccgagg
     1081 tccggcagtt ccggagcgag agtgcgcttc gcgcgtcggc gccgtttctt cccgtcgtcg
     1141 tcggcagcgc tccggtgctc ctcgtggagc gtcgggccgg gctgcagagc gcgctcgtgg
     1201 gcatcggctc cgcagcgggg atcggcgggg gagcgcccct cgcggtcccg gagggactcg
     1261 tcggccgcat cctgatttcc gggcgaacga tctctcgagc gcagctgctg ctcgacgcgt
     1321 cctctgcggc gggcgcccgg atcgtccgaa cgggggagct cggaatcgtg cgcggcgatg
     1381 gacgcggcgc gctgcggttg aaccacattc cgacgacgtc gcgcgttcag cggggggacc
     1441 tgatcgaaac cgcaggcatc gacggcgtct atccgcgcgg gatcccgatc ggccgggtgg
     1501 cctcggtttc gcccggcggc aacctctttc tcgacatcct cgtcgttccg acggcgccgt
     1561 tctcgcgcct caccgacgta ctggttctcg ccccgttcac ggcgcgagtc ccggcggacg
     1621 ggacgagtgt cgcgcggtga agttcgggcg aatcgccctg atcttcgcgg gggcggcggc
     1681 tctcgagttc tttttcggtg gctccaagta ccggaatgcc tggccggtcg actggctgct
     1741 cgtcgcgacg gcggtcgtcg cccggtcggg aggattcggc cgcgcggtcc tgaccggcgc
     1801 agcggcgggg ttgctggaag acgcgctcac ggagagcctg ctcggaatga acgcgttcgc
     1861 gaaggcggct ctgggctacg tgctcgcctt cgtctccgtg cgcgtcattt tcggcggcgc
     1921 gatcgcggtc ggcggcgtga tcgcgctctg ctcgctcgcg aacgacgcag tcgtcgccgt
     1981 cctgcgggcg ctcctcctcg actcgccgat cgtgctgggc gcgccgtatt ccctctggcg
     2041 agccgccgcg acgggagtga cggccggagc gctggaggcc gcgtggacct ttccgtggcg
     2101 ggagtggcgc gaaaggcgga ggctgcgcag actgcggtga tcgaacggcg tgggccggtt
     2161 gcatgctgaa agtccgggag aatcgaagag a
//
LOCUS       DASWAV010000291         8133 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U2665
            k141_16440, whole genome shotgun sequence.
ACCESSION   DASWAV010000291 DASWAV010000000
VERSION     DASWAV010000291.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960316
            Sequence Read Archive: SRR10260007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 8133)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8133)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:47:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,929
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8133
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_16440"
                     /isolate="SMAG_U2665"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.36444 N 132.31889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..493)
                     /locus_tag="VF958_06020"
     CDS             complement(<1..493)
                     /locus_tag="VF958_06020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9148703.1"
                     /translation="MTAAAAVLSAGEWRRKAVHAGFGLFALSLRWLDWEAGAALALAA
                     LIFNVFVMPRIGRGLYRDAQRRRDTGIVSYAVMVLLLIVVLRDHYLTVAAAVWAMMAF
                     GDPAAAAAGELLGGPALPWNRGKRWIGLAANWAAGATASILVFWFVSARPLDRLAAVL
                     LILG"
     gene            complement(551..1483)
                     /gene="rfbD"
                     /locus_tag="VF958_06025"
     CDS             complement(551..1483)
                     /gene="rfbD"
                     /locus_tag="VF958_06025"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004512427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008831 - dTDP-4-dehydrorhamnose reductase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HEX9148704.1"
                     /translation="MADAAVAPVEAQPGLLASPRLMPGERPRVLVTGASGMLGSDLSP
                     ALAGAGYDVYPRPRAALDVTNESHVASAFSELDPHVVVNCAAFTRVDACETDPAAWAI
                     NVRGVGILARGCSRHGARLVQISTDFVFDGGKGEPYAEEDETAPLSVYGLTKRAGEEA
                     ALESPSALVVRASWLFGRSGWNFIEAILKQAEEGRRELSVVDDQRGKPTATTDLSEAI
                     VALLAANASGIFHFANRGEVTWYEFAREILLLAGRNDVNVRPTSSVSLARPARRPGYS
                     VLDTSKYEALTGRTIRHFREPLMEYVAGRARPEA"
     gene            complement(1500..2546)
                     /gene="rfbB"
                     /locus_tag="VF958_06030"
     CDS             complement(1500..2546)
                     /gene="rfbB"
                     /locus_tag="VF958_06030"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019418141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008460 - dTDP-glucose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HEX9148705.1"
                     /translation="MKVLVTGGCGFIGSNFIRHLFESHGDSVSVVNLDKLTYAANPAN
                     LADVAHRADYRFIRGDITDPVVVRHAMEGCDAVVNFAAETHVDRSLLGDASFIETDVR
                     GVFVLLEEARRRGGLTRFIQISTDEVYGSIASGSFHEESALNPRNPYAASKAGGDRLA
                     YSYWASYGLPVVITRASNNYGPYQYPEKLIPLFVTNAIDDHPLPLYGDGRNVRDWLYV
                     KDHAAAIDFLLEKGEPGETYNIAGGNEAENIAITKRVLSLLRKPESLIRFVDDRPGHD
                     RRYSLDAGKLARLGFTSATPFDEGLAETVRWYRDREDWWRPIKDRDTAYREYYRNQYG
                     DRLATSSTTAKPPG"
     gene            complement(2543..3607)
                     /locus_tag="VF958_06035"
     CDS             complement(2543..3607)
                     /locus_tag="VF958_06035"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012257837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008879 - glucose-1-phosphate
                     thymidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase"
                     /protein_id="HEX9148706.1"
                     /translation="MKGLILSGGKGTRLRPLTYTRAKQLVPVANKPVLFYGVEAIVAA
                     GIRKIGVVVGETRDEIREALGDGSRFGAEITYIEQEAPLGLAHAVLISEPFLKGEPFV
                     MYLGDNLIADGITSLVEEYRRIGCNSQILLAKVPNPEQFGVAELADGKVIRLTEKPKE
                     PRSNLALVGVYMFDDSIFEAVRSIKPSSRKELEITDAIQWLIDHGKSVHPHLVAGWWK
                     DTGKVEDMLEANRIILDTFTASAPEGIDEGSRVEGKVVIERGARIFASTIRGPAIIGA
                     GTEIRSAFVGPYTSIGPDCRVERCEIENSIVLEGSSLERIPGRIADSLIGKHVRIHPG
                     AERPRVHRFLVSDNSEIGIL"
     gene            complement(3699..6014)
                     /gene="lptD"
                     /locus_tag="VF958_06040"
     CDS             complement(3699..6014)
                     /gene="lptD"
                     /locus_tag="VF958_06040"
                     /inference="COORDINATES: protein motif:HMM:NF016348.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0010033 - response to organic substance
                     [Evidence IEA];
                     GO_process: GO:0061024 - membrane organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly protein LptD"
                     /protein_id="HEX9148707.1"
                     /translation="MRPTARTGRIAGGLLLFLLASAASAQFGSSDPFESRFKTKPSFE
                     IRFRVPEKGGEVRLTTKNPVHYEKDVFWEGSGEVTIEYQDVKITADRARYDFPTKTAT
                     LEGNVVIDQGPTRLAGSRGVFHVEDKTGRLENATADLAPTYHVVAESIEKVGEATYRI
                     EKGIFTSCNLPRPEWSFYLSEASVTLDDYARMKNVSFRAGPVPVLFTPWLVWPTKQDR
                     ASGFLVPGIGYNGSRGAFLGLSYYWVTGRSTDSTTQLDMYSRGAIGVGEEFRWTPRPE
                     SAGLFQGYAIRDPEATVCVPIEDAPEGGAACTTVDGRIGVFTHRKKTRWKAKLDHVSD
                     DLPFGFRGVLSVRQYSDEQFLRDFERAFALSSSRQTLSRGFLTKNFGDDSVNLRVERS
                     ETFFGTKVVQERLPSLEYSRRTSRIGRSPFFLALESSLSYLYVNRGRGLPQGEYGRAD
                     VHPTISIPWKTVPWLAVTARGGGRWTEYSDSTDEARTLFTGDALSRTYSEAGVSVVGP
                     SFSRIYDGSIGPFGKFKHVIEPRADYEYVSEVGDPLRIPVFDEVDTALGRNQVRYAIV
                     NRLLARPAESKAGGATEIASLEIAQTHAFTQPQLPSGTSSTFESFAAKTGPIEGALRV
                     APGSLFSLDGRTAFDTHVGRITAVSVATSINWKTNYVNATWFGSQPAPVGSSTVSPRT
                     DQIRLAAGLDLGKALRVDTQLNYDAQQSRLLEDRSLLTFNGSCYTLFLEVRQLRLPPQ
                     TRRDYRFVVNLKDVGQLLDVNGSLDRIFGGR"
     gene            complement(6032..7204)
                     /locus_tag="VF958_06045"
     CDS             complement(6032..7204)
                     /locus_tag="VF958_06045"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX9148708.1"
                     /translation="MTPAFDLILGGVPRFGAEGPWAWRLARKFARRSPARRVFEISGR
                     RPLVSDLAGEAPFVLVWADPEAYLAPAAAERLVTTLEASPAADLVLPVSNEAWTDEVL
                     RAPAFAYSTPSGLLEAARLAADSGASPFPLVSPRSPVYAARRAVLAALPETLALEDAP
                     TAMRLAGGCALADPCAYLHRYAEMDAQAREDLAEKIPAGARAVLDVGCSGGATAPALR
                     RRGVTRIVGIEPDTDDAARAAHVYDRVVTTTLEEVSSGEFLAEFDAVLFGDVLEHLVD
                     PSAALVHVRPWLTSGGAVVASVPNVGHWSIVADLLAGRFDYIPYSLLSGTHVRHFTRA
                     TVRDLFEASGYSIVSVDTVRLPPSPEGAAKLALLSSWPEASEDLAVAEFVIVAKPA"
     gene            complement(7281..>8133)
                     /locus_tag="VF958_06050"
     CDS             complement(7281..>8133)
                     /locus_tag="VF958_06050"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020740158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006635 - fatty acid beta-oxidation
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="thiolase family protein"
                     /protein_id="HEX9148709.1"
                     /translation="GMESMSNAPYLLERARTGLRMGNGVLVDSMIKDGLWDPYSDVHM
                     GNCAEMCVSKYSFTREEQDAFARESYRRAQQAAQSGLAAEEIVPVDVPQKKGGGSAAA
                     RVDADEEPFAAPLEKIGTLKPAFQKDGSVTAANSSKINDGAAALVVASAEWAARRGAK
                     PLARIVAYGGVAQAPEWFTTAPVGAIRRLLEKTGLSVSDIDLFEINEAFAAVALAAIR
                     ELDLDPAKVNVRGGAVALGHPIGASGARILTTLVHALRHGGKKRGIAAICIGGGEATA
                     MLVDAET"
BASE COUNT         1382 a         2730 c         2732 g         1289 t
ORIGIN      
        1 caccgagaat gagcaacacg gcggcgagac gatcgagcgg ccgcgcggaa acgaaccaga
       61 acacgaggat ggaggcggtc gcgccggcgg cccagttcgc ggcgagaccg atccagcgct
      121 tgccgcggtt ccagggaagc gcgggtccgc cgaggagttc accggcggcg gccgcggccg
      181 gatctccgaa ggccatcatg gcccagaccg cagcggcgac agtcaggtag tgatcacgga
      241 gaacgacgat caggagaagg accatgaccg cgtaggagac gattcccgtg tcgcgccgac
      301 gttgcgcgtc ccggtagagc ccacggccga tcctcggcat tacgaacaca ttgaagatga
      361 gagcggcgag cgcgagggcc gcccccgcct cccagtcgag ccaccgaaga gacagcgcga
      421 agaggccgaa accggcgtga acggccttgc gtctccactc ccccgcgctc agcactgcag
      481 cggcggcagt caccccctca ccctggccct ctcccggagg gagagggaac gaagcaccgc
      541 ggccgcagtg tcacgcctcc ggcctcgcgc ggcccgcgac gtactccatg agcggctcgc
      601 ggaagtgccg gatcgtccgt cccgtcaaag cctcgtactt cgacgtatcg aggacggagt
      661 aacccggccg ccgcgccgga cgcgcgaggc tgaccgagct cgtcggtcgg acgttcacgt
      721 cgttgcgccc cgccaggagc aggatctcgc gtgcgaactc gtaccaggtc acctcgccgc
      781 ggttggcgaa gtggaagatc cccgatgcgt tggccgccaa cagagcgacg atggcctccg
      841 aaaggtccgt cgtcgccgtg ggctttccgc gctggtcgtc gacgaccgag agctcccgcc
      901 ggccttcctc agcctgcttg agaatcgctt cgatgaagtt ccagccgctc cggccgaaga
      961 gccacgacgc ccgcacgacg agcgccgacg gagactccag agccgcctcc tcgcccgccc
     1021 gcttggtgag cccgtatacc gagagcggcg cggtctcgtc ctcctccgca tacggttcgc
     1081 ccttcccgcc gtcgaacacg aagtccgtcg agatctggac gagccgggct ccgtgccggc
     1141 tgcacccgcg ggccaggatg ccgacgccgc ggacgttaat cgcccacgcg gcgggatccg
     1201 tctcgcacgc atcgaccctc gtgaaggccg cgcagttcac gacgacgtgc gggtcgagct
     1261 cgctgaacgc agaagcgaca tgggactcgt tggtcacgtc gagcgccgca cgcggcctcg
     1321 gatagacgtc gtaccccgcc ccggcgagcg cgggcgagag atccgaaccg agcattccgg
     1381 acgcccccgt gacgagaacc cgcggccgct ccccgggcat gagacgcggc gacgcgagca
     1441 gccccggctg cgcctccacg ggggcgacgg cggcgtccgc caagacgatc tcgatccttc
     1501 taccccggag gcttcgccgt cgtcgaagac gtcgccagcc ggtcgccgta ctggttgcgg
     1561 tagtactcgc ggtatgcggt gtcccggtcc ttgatcgggc gccaccaatc ttcccgatcg
     1621 cggtaccagc gcaccgtctc ggcgagcccc tcgtcgaacg gcgtcgcgct cgtgaagccg
     1681 agacgcgcga gttttccggc gtcgagcgag taacgccggt cgtgccccgg ccggtcgtcg
     1741 acgaagcgga tcaggctctc cggcttgcgg aggagggaga gaactctctt cgtgatcgcg
     1801 atgttctccg cttcgttgcc tccggcgatg ttgtaggtct cgcccggctc gcccttctcg
     1861 agcagaaagt cgatcgcggc ggcgtggtcc ttcacgtaca gccagtcgcg cacgttccgg
     1921 ccgtcgccgt aaagcgggag cggatgatcg tcgatcgcgt tcgtcacgaa cagcgggatc
     1981 agcttttcgg gatactgata cgggccgtag ttgttggagg cccgggtgat cacgaccgga
     2041 agcccgtacg acgcccagta cgagtacgcg agccggtcgc cacccgcctt cgacgccgca
     2101 tagggattgc gcgggttcag cgccgactcc tcgtggaacg agccgctggc gatcgagccg
     2161 tagacctcgt ccgtcgagat ctggatgaac cgcgtgagcc cgccgcggcg ccgggcttcc
     2221 tcgagaagga cgaacacgcc gcgaacgtcg gtctcgatga acgaggcgtc gccgaggagc
     2281 gaccggtcga cgtgggtctc cgcggcgaag ttgacgaccg cgtcgcaacc ctccatcgcg
     2341 tggcgtacga cgaccggatc cgtgatgtct ccccggatga agcgatagtc cgcgcggtgc
     2401 gcgacgtcgg cgaggttcgc agggttcgcc gcatacgtga gcttgtcgag gttcacgacc
     2461 gagaccgagt ccccatggga ttcgaacagg tggcggatga agttcgagcc gatgaaaccg
     2521 cacccgccgg tgacgaggac cttcacagga tgccgatctc ggagttgtcg ctgacgagaa
     2581 agcggtgaac gcgcggacgt tcggcgcccg gatggatccg gacgtgcttg ccgatcaggg
     2641 aatctgctat ccggcctgga atccgctcga gggacgagcc ctcgagaacg atcgagttct
     2701 cgatttcgca gcgttcgacc cggcagtcag ggccaatgga ggtgtagggc ccgacgaacg
     2761 cgctccggat ctcggtgccc gctccgatga tcgccgggcc gcggatcgtg ctcgcaaaaa
     2821 tccgagcgcc ccgctcgatc acgacctttc cctcgacacg cgacccctcg tcgatcccct
     2881 ccggcgcgct cgccgtgaaa gtgtcgagga tgatgcggtt cgcttccagc atgtcttcga
     2941 ctttccccgt gtctttccac caaccggcga cgaggtgcgg gtggacgctc ttgccgtggt
     3001 cgatcagcca ctggatcgcg tcggtgatct ccagctcctt tcgagagctg ggtttgatcg
     3061 accggacggc ttcgaagatg gagtcgtcga acatgtagac accgacgagc gcgaggttcg
     3121 agcgcggctc cttcggcttc tcggtcaggc ggatgacctt cccgtcggcg agctcggcga
     3181 cgccgaactg ctcggggttc ggcactttcg cgaggaggat ctgcgagttg cagccgatcc
     3241 ggcgatattc ctccacgaga gaggtgatcc cgtcggcgat caggttgtcg ccgaggtaca
     3301 tgacgaaggg ctcacctttc aggaacggct ccgagatgag gactgcgtgg gcgagcccga
     3361 ggggcgcttc ctgttcgatg tacgtgatct cggcaccgaa acgcgagccg tcgccgagcg
     3421 cttcacggat ctcgtcgcga gtctcgccga cgaccacccc gatctttcgg atcccggcgg
     3481 caacgatcgc ctcgacgccg tagaagagaa cgggcttgtt ggcgaccggc accagctgtt
     3541 tcgcgcgcgt atacgtcagg ggacgcaggc gggtcccctt gccgcccgag aggatgaggc
     3601 ctttcatttt cggcaatcgt aattcggaaa tcggaaatcg gaaatcggaa acagagccac
     3661 cctgcccgat ttccgactcc cgattcccga ttcccgtctc aacgtccccc gaagatgcga
     3721 tcgagcgagc cgttcacgtc gagtagctgc cccacgtcct tcaggttcac gacgaaacgg
     3781 tagtcgcgcc gcgtctgcgg cggcagccgg agctggcgca cttcgagaaa gagcgtgtag
     3841 caggatccgt tgaaggtcag gagagatcgg tcctcgagaa gacggctctg ctgcgcgtcg
     3901 tagttcaact gggtatcgac ccgcagagct ttgccgagat cgaggcccgc ggcgaggcgg
     3961 atctggtcgg tgcgcggcga gacggtcgag ctcccgaccg gcgccggctg gctcccgaac
     4021 caggtcgcgt tcacgtagtt cgtcttccag ttgatcgacg tcgcgacgga gaccgccgtg
     4081 atcctgccga cgtgcgtgtc gaaagccgtg cgcccgtcga gcgaaaacag gctgccaggc
     4141 gccacccgaa gcgctccctc gatggggccg gttttcgccg cgaacgattc gaacgtgctc
     4201 gacgttccgg aaggcagctg cggctgggtg aaggcatgag tctgcgcgat ttcgagcgaa
     4261 gcgatttcgg tcgcacctcc cgctttgctc tcggcggggc gcgcgagcag tcggttgacg
     4321 atcgcgtacc ggacctggtt gcgcccgagg gccgtgtcca cctcgtcgaa gacagggata
     4381 cgcaaggggt cgccgacctc tgacacgtac tcgtagtccg cacgcggctc gatgacgtgt
     4441 ttgaacttgc cgaacgggcc gatcgagccg tcgtagatgc gcgagaacga cggccccacg
     4501 acggagactc ccgcctcgct gtatgtgcgc gagagagcgt cgccggtgaa aagcgtccgc
     4561 gcctcgtcgg tcgagtcgga gtactccgtc caacgtccgc cgccgcgcgc cgtgaccgcg
     4621 agccagggga cggtcttcca cgggatggag atcgtcgggt ggacgtccgc gcgcccgtac
     4681 tcgccctgcg gcaggcctcg gccgcggttg acgtagagat aagacagcga cgactcgagg
     4741 gcgaggaaga agggggaacg cccgatgcgc gacgtgcggc gggagtattc gagcgagggc
     4801 agcctctcct gcacgacttt cgtgccgaag aaggtttcgc tccgctcgac acgcaggttg
     4861 acggaatcgt cgccgaagtt cttcgtcaga aagccgcgcg agagcgtctg gcgcgacgag
     4921 gagagcgcga atgcgcgctc gaagtcgcgg aggaactgct cgtccgaata ctgccgcacc
     4981 gacaggacac cgcggaatcc gaacggcagg tcgtccgata cgtgatcgag cttcgccttc
     5041 caacgcgtct tcttccgatg cgtgaacact ccgatgcgcc cgtcgaccgt cgtgcaggcg
     5101 gcgcccccct ccggcgcgtc ctcgatcggg acgcagactg tcgcctccgg gtcgcggatc
     5161 gcgtagccct gaaagagtcc ggcggactcg gggcgcggcg tccagcggaa ctcctcgccg
     5221 acgccgatcg cgccgcgcga atacatgtcg agctgggtcg tcgagtcggt cgagcgcccg
     5281 gtcacccagt agtacgagag gccgagaaac gctccccggc ttccgttgta cccgatcccc
     5341 gggacgagga aaccggacgc gcggtcctgc ttcgtgggcc atacgagcca cggagtgaag
     5401 agaaccggga ccggcccggc tcggaaagag acgtttttca ttcgcgcgta gtcgtccagg
     5461 gtcacgctcg cctcggagag atagaacgac cactccggcc gcggcagatt gcaggaagta
     5521 aagatcccct tttcgatgcg atacgtcgcc tctcccacct tttcgatcga ttccgcgacg
     5581 acgtggtagg tcggcgcgag gtcggcggtg gcgttctcca gacgccccgt cttgtcttcg
     5641 acgtgaaaga cgccgcggct tccggcgagc cgcgtgggtc cctgatcgat gacgacgttg
     5701 ccttcgaggg tcgccgtctt cgtcggaaag tcgtaccggg cgcggtccgc cgtgatcttg
     5761 acgtcctggt actcgatcgt gacctcgccg cttccctccc agaatacgtc cttttcgtag
     5821 tgcaccggat tcttcgtcgt gaggcggacc tcgcccccct tttccggaac gcggaagcgg
     5881 atctcgaagc tcggcttcgt cttgaagcgc gactcgaagg ggtcgctcga tccgaattgc
     5941 gcggaggccg cggatgccaa caggaacagg aggagtcctc cggcgatcct tccggtccgg
     6001 gccgttggtc tcacgtctca ggtcttacgt ttcacgccgg ctttgcgacg atgacgaact
     6061 ccgcgacggc gaggtcctcc gacgcctcgg gccagctcga cagaagcgcg agcttcgcgg
     6121 cgccttccgg agacggcgga agacgcacgg tatccaccga cacgatcgaa taccccgagg
     6181 cctcgaacag gtcgcggacc gtcgcgcgcg tgaagtggcg cacgtgggtt cccgagagga
     6241 gcgagtaggg gatgtagtcg aaccgcccgg cgaggagatc cgcgacgatc gaccagtgcc
     6301 cgacgttcgg caccgaggcg acgacggcgc ccccggaagt gagccagggc cgaacgtgca
     6361 cgagcgcggc ggaggggtcc accagatgct cgagcacgtc gccgaaaaga actgcgtcga
     6421 attcggcgag gaactcgccg gaagaaacct cctccagagt cgtcgtgacg acccggtcgt
     6481 agacgtgcgc cgcgcgcgcc gcgtcgtccg tgtccggctc gatgcccacg atccgggtca
     6541 ccccgcggcg ccggagcgcc ggtgcagtcg cgcccccgga acaaccgacg tccagcacgg
     6601 ctcgcgcccc ggccgggatt ttctcggcaa ggtcctcacg agcctgggca tccatctctg
     6661 cgtagcggtg caggtacgcg cacggatccg cgagtgcaca gccgccggcc aggcgcatgg
     6721 cggtcggcgc gtcctcgagc gcaagagtct cggggagtgc tgcgagcacc gcgcggcgcg
     6781 cggcgtagac gggcgagcgc ggggaaacga gcgggaaagg ggacgccccg gagtccgccg
     6841 cgagacgcgc cgcctccagg agacctgacg gagtggagta cgcgaaggcg ggcgcccgga
     6901 gcacctcgtc ggtccacgct tcgttcgaaa ccggcagcac gagatcggcg gcgggagacg
     6961 cctcgagcgt cgtaacgagc cgctccgccg ccgccggcgc caggtacgcc tcgggatccg
     7021 cccacacgag gacgaagggt gcctctcccg cgaggtcgga gacgagcggt cggcgccccg
     7081 aaatttcgaa gactcgtcgc gcggggctcc ggcgggcgaa cttccgcgcc agccgccagg
     7141 cccaggggcc ttctgcgccg aaccggggaa cgccgccgag aatcaagtcg aaagccggtg
     7201 tcatggagga gcccggaaat cggtaatcgg aaatcgtgaa tcgagtcgag cctttcgatc
     7261 gccgatttcc gattcccggt tcacgtctcc gcgtccacga gcatcgccgt cgcctcgccg
     7321 ccaccgatgc agatcgccgc gatcccacgc ttcttgccgc cgtgacgcag cgcgtgcacc
     7381 agcgtcgtga gaatccgggc gcccgacgcg ccgatcgggt gtccgagagc taccgcgccc
     7441 ccgcggacgt tgaccttcgc gggatcgagg tcgagctcgc gaatggccgc cagcgcgacc
     7501 gccgcgaacg cctcgttgat ctcgaagagg tcgatgtcag agaccgagag cccggtcttt
     7561 tcgaggagcc ggcggatcgc gcccaccggg gcggtcgtga accactcggg cgcctgagcg
     7621 acccccccgt acgcaacgat cctcgcgagc ggctttgcac cgcggcgggc cgcccactcg
     7681 gcggaggcga ccacgagtgc cgcggcgccg tcgttgatct tcgacgagtt cgccgcggtg
     7741 acggaaccgt ccttctggaa cgcgggcttc aacgtcccga tcttctcgag cggcgcggcg
     7801 aagggctctt cgtcggcgtc cacacgcgca gcggcggacc cccccccctt cttctgcggc
     7861 acgtccaccg ggacgatctc ctccgccgcc agacccgact gcgcagcctg ttgcgcccgc
     7921 cggtacgact cgcgcgcgaa ggcgtcctgt tcttcgcggg tgaaggagta tttcgagacg
     7981 cacatctccg cgcagttccc catgtgaacg tcgctgtacg gatcccagag gccgtccttg
     8041 atcatcgagt cgacgagcac gccgttgccc atgcggaggc cggtccgggc gcgctccagc
     8101 agatacggcg cgttggacat cgactccatg ccg
//
LOCUS       DASWAV010000292         2945 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U2665
            k141_334296, whole genome shotgun sequence.
ACCESSION   DASWAV010000292 DASWAV010000000
VERSION     DASWAV010000292.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960316
            Sequence Read Archive: SRR10260007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2945)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2945)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:47:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,929
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2945
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_334296"
                     /isolate="SMAG_U2665"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.36444 N 132.31889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..226
                     /locus_tag="VF958_06055"
     CDS             <1..226
                     /locus_tag="VF958_06055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9148710.1"
                     /translation="SERGDTRLEVLGGNLHLLRNPVPDVLLAKTRDGEAELPGRPRDR
                     VAEMFAVGGVDLRVRLQSVLERAEGLPGGE"
     gene            583..1578
                     /locus_tag="VF958_06060"
     CDS             583..1578
                     /locus_tag="VF958_06060"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF024545.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HEX9148711.1"
                     /translation="MRLRISILLSLLLAAATAFAGELVEKIVARVNDRLITSSEFQKR
                     FEVFISSPQAGNNPLEARRKLLKELIDEKLLEERAKELSVQATDEEVEAAVERVKRQY
                     NLATDAEFDAALKTSNLNREDLKRQMRNTITLQKVVGRDISSKLELTDDALRLEYERR
                     KEQLYKVPEQAKVSEIVIRFEPNDEAGRARAAARIEEARTKVAAGTPFADAAKEYSEG
                     NARERGGALGSVSRGELVTALDAAVFSDTPQEYPPPVLLSSSIHLFHVTDRKAAGYRP
                     FADVKEDLRARIADDLYDKKYAEYIEKLRREAFIKIYDPALATTTAPAGEEKKPS"
     gene            1623..2423
                     /locus_tag="VF958_06065"
     CDS             1623..2423
                     /locus_tag="VF958_06065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein HesB"
                     /protein_id="HEX9148712.1"
                     /translation="MRSHGWYDLPPFSYEREKGVLTTVVGGSQVAFRIHRGKLKARAE
                     GLEPSRLRRIARRVFSLDLDLGDAERSFAHEPVLARALGDRGGRMLRAPTLFEDAVKI
                     LFTTNCSWGATRGMVSRLIALAGADARAFPTPESVVRLSTASIEKHVRCGYRAEALRL
                     FARRVASGKLDLSAWENPERPSEQVREAIVEERGFGPYAAEGLLRILGRHDFYALDSW
                     TRQQYRKLYPGKAASTDRSIARRYARFGPHRGLALWLDMTSHWHKPGS"
     gene            2727..>2945
                     /locus_tag="VF958_06070"
     CDS             2727..>2945
                     /locus_tag="VF958_06070"
                     /inference="COORDINATES: protein motif:HMM:NF014439.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HEX9148713.1"
                     /translation="MDRFRGSAPARVDEKGRLKVPSLFRAQIDDAFGPELFVTSVHGR
                     NVLLYPLAIWRALEEKLAALPAVHRAKNK"
BASE COUNT          535 a          942 c          974 g          494 t
ORIGIN      
        1 ttccgagcgc ggtgatacgc gcctggaagt cctcggcgga aaccttcatc tccttcgtaa
       61 tccggtccca gacgtccttc tcgcgaaaac gcgtgacgga gaggccgagc tgcccggccg
      121 cccacgcgat cgcgtcgcgg aaatgttcgc cgtcggcggt gtggatcttc gcgtgcgact
      181 ccagagtgtc ctcgagcgag ccgaggggct tcccggaggc gagtagcagg ccggaggcgg
      241 cgacgtcgga accgcccttc cggatttccg cgatggcggc gcgaacggcc gagagagcga
      301 ggcggcgcga gctcttctcg cagcgttcga gaaggtcctt cgctcgggga agcccgatct
      361 cttcggccgc gtggtagggc tgcttcgagc ccggaatcga cgggtccgca atctcgatcc
      421 ggcggcgcag gaggacttcg ggcgcgaggg gatccccggc gacggcgacg accgtcgccc
      481 agcccgagtg cgaacggaag ccgagggccg caggagggac cgtggcggaa agtctctcat
      541 ggcgcggaat tcgggctttc cggccgtgtt atgaattcac cgatgcggct ccggatttcg
      601 atcctccttt ccctccttct ggcggcggcc accgccttcg ccggcgagct cgtcgagaag
      661 atcgtcgccc gcgtcaacga ccgcttgatc acgagcagcg agttccagaa acggttcgaa
      721 gtcttcatct cctcgccgca ggccggcaac aatccgctgg aggcccggcg gaaactgttg
      781 aaggagctca ttgacgagaa gctcctcgag gaacgcgcga aggaactgtc ggtgcaggcg
      841 acggacgagg aggtcgaggc cgcggtcgag cgggtcaaac ggcagtacaa cctcgccacc
      901 gacgccgagt tcgacgcggc gttgaagacc tcgaacctga accgcgagga cctgaagcgc
      961 cagatgcgca acacgatcac gctccagaag gtcgtcggtc gagacatcag ctcgaagctc
     1021 gaactgaccg atgacgcctt gaggctcgag tacgagcgtc gcaaggagca gctctacaag
     1081 gttccggagc aggcgaaggt gtctgagatc gtgatccggt tcgagccgaa cgacgaagcc
     1141 gggcgcgcgc gcgccgccgc ccggatcgaa gaggcgcgaa cgaaggtcgc agcgggaacg
     1201 ccgttcgcgg acgcggcgaa ggagtactcg gaggggaacg cccgcgagcg ggggggcgcg
     1261 ctcggcagcg tgagccgggg agagctcgtg acggcgctcg acgcggccgt cttctccgac
     1321 accccgcagg agtacccacc gccggttctc ctgtcctctt ccattcacct gtttcacgtc
     1381 acggaccgca aggccgccgg ctaccgtccg ttcgcggacg tgaaggaaga cctgcgcgcc
     1441 cggatcgccg acgatctcta cgacaagaag tacgccgagt acatcgagaa actccggcgc
     1501 gaggcgttca tcaagatcta cgaccctgcc cttgccacca cgacggcgcc ggcgggggaa
     1561 gagaagaaac cgtcgtagcg tcccgcctcg ggctccctcc ccgcttctcg ttcgacgccg
     1621 tcgtgcggtc gcacggctgg tacgacctgc cgccgttcag ctatgagcgg gagaagggcg
     1681 tactcactac ggtcgtcgga ggcagccagg tggcgtttcg aatccatcgg ggaaagctca
     1741 aagcgcgtgc ggaaggtctc gagccctcgc gcctccggcg gattgctcgg cgtgttttct
     1801 cgctcgacct cgacctcggg gacgccgagc gctcgttcgc ccacgagccg gtcctcgctc
     1861 gcgctctcgg agatcgcggt ggacggatgc tgcgagcgcc gacgctcttc gaagacgcgg
     1921 tgaagatcct tttcacaacg aactgctcgt ggggcgcgac gcggggaatg gtgtcgcggc
     1981 tgatcgctct cgccggcgcg gacgcccggg cgtttccgac gcccgaatcg gtcgtgcggc
     2041 tgtcgacggc gtcgatcgaa aagcacgtgc gctgcggata ccgcgcagaa gcgctgcgcc
     2101 tcttcgcgag acgcgtggcg tcgggaaagc tcgatctttc ggcctgggag aacccggagc
     2161 gaccgtcgga acaggtgcga gaagcgatcg tcgaagagag gggattcggg ccttacgcgg
     2221 cggagggact gctccgcatc ctcggccgtc acgatttcta cgcgctcgac tcctggacga
     2281 ggcagcaata ccgaaagctc tacccgggaa aggcggcctc gaccgatcgc tcgatcgccc
     2341 ggcgttacgc acggttcggt ccgcaccgcg ggctcgcgct gtggctcgat atgacgagtc
     2401 actggcacaa accagggagc tgaccgccgc cttctcgttc ggaataccct tcgaatcgcc
     2461 gaatttgctc ggccgacatc caccgcttgg gtgtcgccag ttctacgacc gcaacctgtt
     2521 gagatgcaat gcactagaag gagtgcattc gccttgatgt gaccatcgcg cgtctttacg
     2581 ctgtacttac gcccctaatt ttttcaggta attcgttttc cttgactaac aaacttacgg
     2641 ctcctaacat ccgccgcaag tggcccaaat cggttaaaag tgggagaaag tggcagcgat
     2701 ctccggatcg gtgcaccaat tgaaccatgg atcgctttcg agggagcgct ccggctcgtg
     2761 tcgatgagaa gggccgtctc aaggtcccga gcctgttccg cgcgcagatc gacgacgcct
     2821 tcggaccgga actcttcgtg acttcggtgc acggaaggaa cgtcctcctg tacccgctcg
     2881 cgatctggcg cgccctcgag gagaagctcg cggcgctgcc cgccgtgcac cgcgcgaaga
     2941 acaag
//
LOCUS       DASWAV010000293         2117 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U2665
            k141_176145, whole genome shotgun sequence.
ACCESSION   DASWAV010000293 DASWAV010000000
VERSION     DASWAV010000293.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960316
            Sequence Read Archive: SRR10260007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2117)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2117)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:47:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,929
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2117
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_176145"
                     /isolate="SMAG_U2665"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.36444 N 132.31889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1263
                     /locus_tag="VF958_06075"
     CDS             <1..1263
                     /locus_tag="VF958_06075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9148714.1"
                     /translation="FVSGLARAERLAWSLATGLLVQSVLFVTLVAVFPARARLPVAAA
                     DVTIMAASFLIRRSAGFPSPRLREEGQGEGRALVVTLLAIAAAAWLLFLVSALSEPMW
                     STDYLAIWGLKARTIATTGHVPQRLFSDPALYWAHREYPLLVPFSLAAFASLAGGWDD
                     QALALLFPLCEVATLCALYGFLVRHVSPLAGATAAALTALCFPLYRAVNVGTAEVPFA
                     FALVLVSTAFFDSLAERSRMVLARLAVASLFCVSIKQEGWIFVGLLAAVLATRRRWIP
                     AAAIAIPAVAQWLLLYLLRGPQTRRDFDFTFFQPHHWAQLFPRFFQVLGRMLGTEALA
                     GWLPLAAIVLYLIATRRGAADALLPILPLQILCYAIAFSVSSFDPMYAVDGAFRRIAT
                     TLFPALTLVLAARIPGQAASGVNEPSRS"
     gene            complement(1241..1837)
                     /locus_tag="VF958_06080"
     CDS             complement(1241..1837)
                     /locus_tag="VF958_06080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9148715.1"
                     /translation="MSPIYDQGRFMALAYSYQYARPGRPLFSNAEVWLLESEDANEWK
                     RSRRVSLANVQPPYLQRDCVRGAWMMNPDIARDHAGRYFVTRAYSDNYAGCRVTFPNR
                     VQVYSARDEAALVHGPWNKVVDLGCAELGFQPDSAQILHDGLGRTVANASGALTLTVA
                     VSGGDWTFSLCGREERHPGACGPPPPQRIQEVTIERVR"
BASE COUNT          300 a          753 c          644 g          420 t
ORIGIN      
        1 ttcgtgtccg ggctcgcgag ggcggagcgg ctcgcctgga gcctcgctac gggactcctg
       61 gtccagtcgg tcctgttcgt gaccctcgta gcggtttttc cggcgcgcgc acgcctgccg
      121 gtggccgccg ccgacgtcac gatcatggcg gcgagcttcc tgatccgccg atccgccggt
      181 ttcccctctc cccgcttgcg ggaagagggt cagggtgaag ggcgtgctct cgtcgtcact
      241 ctcctcgcta tcgccgccgc cgcgtggctc ctcttcctcg tctccgcact gtccgagccg
      301 atgtggtcca ccgactacct cgcgatctgg gggctcaagg ccagaacgat agcgacgacc
      361 ggccacgtgc cgcagcgact cttctccgat cccgcgctct actgggcgca ccgggagtat
      421 ccgctcctgg taccgttctc gctcgcggcg ttcgcttcgc tcgccggggg ttgggacgac
      481 caggcgctcg cgctcctttt ccccctgtgc gaggtcgcga ccctgtgcgc tctttacggt
      541 ttcctcgtgc gccacgtatc gccgctcgcg ggcgcgaccg cggcggctct tactgctctc
      601 tgcttcccgc tctaccgcgc ggtcaacgtc gggacagcgg aggtgccatt cgcatttgca
      661 ctggtactcg tttcgacagc gttcttcgat tcgctcgcgg aacggtcgcg aatggtgctc
      721 gcgcgcctcg ccgtcgcatc gctcttctgc gtctcgatca agcaggaggg ctggatcttc
      781 gtgggacttc tcgcggcggt actcgcgaca cggcggcggt ggattccggc cgccgcaatc
      841 gcgattccgg ctgtcgccca gtggctgttg ctctacctgc ttcgcgggcc gcagactcgt
      901 cgcgacttcg acttcacgtt cttccagccg caccactggg cgcagctctt cccgcgcttc
      961 ttccaggtgc tcggccggat gctcgggacc gaagcgcttg ccggctggct gccgctcgcg
     1021 gcgatcgtgc tctacctcat cgcgacccgg cgcggagccg ccgatgcgct cctcccgatc
     1081 ctgccactcc agatcctctg ttacgcgatc gcgttctcgg tgtcgtcgtt cgatccgatg
     1141 tacgccgtcg acggcgcctt ccgccgcatc gccacgactc ttttcccggc gttgacgctc
     1201 gtccttgccg cgcgaattcc gggtcaggcg gcctcgggag tcaacgaacc ctctcgatcg
     1261 tgacctcctg aatgcgctgt ggcggtggag gtccgcacgc gccgggatgc cgctcctccc
     1321 ggccgcacaa cgaaaacgtc cagtctccgc cgctcaccgc gacggtcagc gtgagagcgc
     1381 cggaagcgtt cgcgacggta cgtccgaggc catcgtgaag aatctgagcc gaatccggtt
     1441 gaaacccgag ctcggcacag cccaggtcca cgaccttgtt ccacggcccg tgaacgaggg
     1501 cagcttcatc ccgtgccgaa tagacctgca cgcggttcgg aaaagtgacg cggcatcccg
     1561 cgtagttgtc cgaataagcg cgagtgacga agtaccttcc ggcgtgatcg cgggcgatat
     1621 cgggattcat catccaggct ccccggacgc agtcgcgctg gagatacggt ggctgtacgt
     1681 tcgccaagct cacccgtcgg ctgcgcttcc actcgttcgc gtcctcactc tcgagcagcc
     1741 agacctccgc gttcgaaaaa agcgggcgcc cgggacgcgc gtactggtag gagtaggcca
     1801 gcgccatgaa tcggccctgg tcgtagatgg gactcatgtc cccttcggtc caccaggaac
     1861 gcgggcgagc ggaatcacaa ggccatccct gatcgcacca ccaactccca tcgcgacaat
     1921 gcgggtcctc ggtggagacc tcggagcaat ccggcaggat gcgccggtgc cccgtgaaac
     1981 ccactccgtc attcgtgtaa gccatccact ttccgtcgct gccgcaccgc tctccgctgc
     2041 agctcgagat ccgctgcgtg gaatagaaaa ttgcgccgtc gtatcgtcgg ccggtagcag
     2101 gatcccgaaa cagtctc
//
LOCUS       DASWAV010000294         3096 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U2665
            k141_573200, whole genome shotgun sequence.
ACCESSION   DASWAV010000294 DASWAV010000000
VERSION     DASWAV010000294.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960316
            Sequence Read Archive: SRR10260007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 3096)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3096)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:47:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,929
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3096
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_573200"
                     /isolate="SMAG_U2665"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.36444 N 132.31889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..430)
                     /locus_tag="VF958_06085"
     rRNA            complement(<1..430)
                     /locus_tag="VF958_06085"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
     gene            complement(960..2498)
                     /locus_tag="VF958_06090"
     CDS             complement(960..2498)
                     /locus_tag="VF958_06090"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HEX9148716.1"
                     /translation="MTEAPATSVPRGPVERWLLRHTWLTVVVVVAAGLAIRIAAIRGT
                     RLGSDEAVHFQLVNVSSIWEVYRATLTNAHPPLFFFLLHLWHLIGTSEFFLRLLPVLF
                     GTALLGVAYRWADHLFGKSAALFTLAILAFSPAVVALSAEVRGYTLLLFLMVSALLVL
                     ERAIERRSVIAMLGFSGLLYLAILTHYSAFWFTLAAFTYVLIRIRAEHLPKSLIATWL
                     VFQVGAAALYAFLYFSHLVNLRGSELERAARNHADYFYAEQESIFTFLARQTSAFFRA
                     LFGSPAAAFVGLTAVALGIALLVSRKRPAALLLALPFLLGAAGGLARLYPYGGTRHAA
                     YLMPFAAVAFGVAAAATTGRLWPALLVGVMLAGSSGSEIASRRPNITEMKAAVEAIRA
                     AAAPGSLLFSDLHTGLTLSYYLGGSDFFHENPTPTHFWKSGAGGYRLVGSHQWAFNAK
                     RFSSELQQFIESYSLSSGQTVWIIHVGPELNPAQVLSQQYPTARLTHKFQFGDIAVVE
                     VRLP"
     gene            complement(2505..>3096)
                     /gene="purN"
                     /locus_tag="VF958_06095"
     CDS             complement(2505..>3096)
                     /gene="purN"
                     /locus_tag="VF958_06095"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020708058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HEX9148717.1"
                     /translation="VLLSGRGSNFEAIADAVESGRIPNAEIVAVISDVSEARGLDRAR
                     ERGLAAFSVERERFGSRAEHESTILRILEETRPDLICLAGYMRILSPQFVERWRGRIL
                     NIHPSLLPRYRGLSPQKRALEAGDHESGCTVHIVDEGTDTGPVVLQKRVPIEPGDTEE
                     TLSARILAQEHQAYPEAIARTLEGLEHAPEPSRPDR"
BASE COUNT          636 a          937 c          982 g          541 t
ORIGIN      
        1 gggccttcat cctccacgcg gcgttgctgc gtcaggcttt cgcccattgc gcaagattcc
       61 ccactgctgc ctcccgtagg agtccggacc gtgtctcagt tccggtgtga ccgatcgccc
      121 tctcaggccg gttaccgatc gtcgccttgg tgagccgtta cctcaccaac tagctaatcg
      181 ggcgcgggcc catccggtag cgcgagatct tgcgaccccg ctttgatcgt caggactttc
      241 gtcccaaaga tgtcatgcgg tattagctcg agtttccccg aggtattccc cacttccggg
      301 taggttaccc acgtgttact cacccgtccg ccgctcgact cagggagttg cccccctggc
      361 ctcgctcgac ttgcatgtgt taagcacgcc gccagcgttc attctgagcc aggatcaaac
      421 tctccactta aatctgtttc agtcgaccac gaatgaatcc gcagccggtt gaagaagagt
      481 tgtgaacctg cttttcagac aaaacaggaa actctgcttt tttcaaaagc agaaaaagac
      541 gtctggcacg ctctactcag ttttcaagga accggatccg gtttgacctg gacgcgctgt
      601 cccaccagcg caactacttg ataacaggct caaaagaccg aaattccagc ttaccgattt
      661 ttcgggcccg gtcaacgaga tcttcgcgtc cgaccgccca gaggcgggtc gggcgtcgca
      721 aaccgggctt ggaaagagct gggaccccct cgcgggggga tgcgcaatat aggggttcgg
      781 aggtgtgctg tcaagcaatt cccaaaaagc catcaagtac cgtagttcca agcagttaga
      841 cgagacctgg atgcacaggt tcaggtgcag ccggcgcccc cctccagcaa tccgcctgag
      901 cggcgaggga gacccgcgcc gggacggttg ccgcgaaggg cgcccgccag gcgccccggt
      961 caaggcagac gaacctcaac gaccgcgata tcgccaaatt ggaacttgtg cgtcagtctc
     1021 gcggtcgggt actgctgaga aagaacctgt gccgggttca gttccggccc gacgtgaatg
     1081 atccagactg tctgcccgga ggagagcgag tacgactcga tgaattgctg cagctcggag
     1141 gaaaatcgtt tggcgttgaa ggcccactga tgcgagccga cgagccggta ccccccggcg
     1201 ccgctcttcc agaagtgcgt tggggtgggg ttctcgtgga agaagtcact cccgccgaga
     1261 tagtaggaaa gcgtgaggcc cgtgtggaga tccgaaaata gcaggctgcc gggggctgcg
     1321 gctgcgcgaa tcgcctcgac agcagctttc atctcggtga tgttcggcct tcgcgaggcg
     1381 atttccgagc cggaggaacc tgccagcatg acccccacca gaagagcggg ccagagccgg
     1441 cccgtcgttg ccgccgcggc gactccgaat gcgaccgctg caaacggcat gagataggcg
     1501 gcgtgtcgcg ttccgccgta gggatagagg cgagcgagcc cacccgccgc gccgagcaga
     1561 aaggggagcg cgaggaggag cgccgccgga cgttttcttg aaacaagcag agcgatcccc
     1621 aacgcgaccg ccgtgagccc cacaaaagcg gcggcaggtg aaccgaagag cgcacggaag
     1681 aaggcgctcg tctgtcttgc gagaaatgtg aatatgctct cctgttccgc gtagaagtaa
     1741 tctgcgtgat tcctcgccgc ccgttcgagc tcgcttcctc tcaggttcac gaggtggctg
     1801 aaatagagaa acgcatagag agccgcggcg ccgacctgga aaacgagcca cgtcgcgatc
     1861 agcgatttcg gaagatgctc ggcgcggatc cggatcaata cataggtgaa ggccgccagc
     1921 gtgaaccaga aagccgagta gtgagtcagg atggcgagat agagcagacc ggagaaaccg
     1981 agcatagcga tgacggagcg cctctcgatc gctcgctcca gaaccagaag cgcggaaacc
     2041 atcagaaaaa ggagcaacgt gtatccgcgc acctccgcgg aaagggcgac caccgcgggc
     2101 gagaatgcga ggatggcgag agtgaagagc gcggcgctct ttccgaagag gtgatccgcc
     2161 caccggtagg cgactcccag aagtgcggtc ccgaagagaa ccggaagcag gcggagaaag
     2221 aactcggaag tcccgatgag gtgccagagg tggaggagaa aaaagaagag cggagggtgg
     2281 gcattcgtga gcgtcgctcg gtacacctcc catatcgacg atacgttgac gagctggaag
     2341 tgaacggctt cgtcgctgcc gaggcgagtc cctcggatcg ccgcgatccg gatcgcgaga
     2401 cctgccgcta cgacgacgac gacggtcagc cacgtgtgtc gaaggagcca gcgctcgacg
     2461 gggccgcgcg ggactgaagt cgctggcgcc tcggtcacct cgacctaccg atctggtctg
     2521 ctgggttcgg gcgcatgctc gagaccttcg agcgtcctgg cgatcgcttc cggataggcc
     2581 tgatgctctt gcgcgaggat tctggcggag agtgtttcct ccgtgtcgcc cggctcgatc
     2641 gggactcgct tctggagaac gaccggcccc gtatccgtgc cctcgtcgac gatgtgcacc
     2701 gtgcacccgg actcgtggtc tcccgcctcg agggcccttt tctgaggcga gagtccgcgg
     2761 tacctgggaa ggagcgacgg gtggatgttg aggatccgcc cgcgccagcg ctcgacgaac
     2821 tgcggcgaga ggattcgcat gtacccggcc agacagatga gatccggccg cgtctcttcc
     2881 aggattcgaa ggatcgtact ctcgtgctcc gcgcggctgc cgaatcgctc gcgctctacg
     2941 gaaaaggcgg cgagcccgcg ctctctcgcg cgatcgagtc cgcgtgcttc ggagacgtcc
     3001 gagatcacgg cgacgatctc agcgtttgga atccgccccg actcgaccgc gtcggcgatg
     3061 gcctcgaagt tggatccccg gcccgagagc aggacc
//
LOCUS       DASWAV010000295         4022 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U2665
            k141_573210, whole genome shotgun sequence.
ACCESSION   DASWAV010000295 DASWAV010000000
VERSION     DASWAV010000295.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960316
            Sequence Read Archive: SRR10260007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 4022)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4022)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:47:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,929
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4022
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_573210"
                     /isolate="SMAG_U2665"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.36444 N 132.31889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..242)
                     /locus_tag="VF958_06100"
     CDS             complement(<1..242)
                     /locus_tag="VF958_06100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9148718.1"
                     /translation="MSRRGDHTERVVLWLLLALGAALMLLAAGTARGDETANRTDRIS
                     ANLPSGATLRLDNVSGDVIAVPGREFTAVATIVVAA"
     gene            complement(302..1249)
                     /locus_tag="VF958_06105"
     CDS             complement(302..1249)
                     /locus_tag="VF958_06105"
                     /inference="COORDINATES: protein motif:HMM:NF025030.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HEX9148719.1"
                     /translation="MECRELTEYAIGRLTGEASSREREELQGHLAGCESCRAELATVE
                     ETWQTLGSDPEAVVTPEYRARTLALLEDEIARRRIREFRPRSRAWRALGQAAAVVLAA
                     AAGFLAAKGGLGTAPKPGNGVAAAGSAVPPSWPDLSENPSLTNLSYKTDADGRVGIAF
                     DATTHHSITGRPDDPRVSSLLAYLLSRNETAGEKSRAIELVSGNFGPGAAVASPEIVD
                     ALAATLKRDRNPGVRKKAAEALASFRSTSQIRAALLDALANDPNPGVRLVAVDALAAA
                     AKEAPDPKTIQSLRDKAFDPSENGFVRARAASALKAIDL"
     gene            complement(1231..1791)
                     /locus_tag="VF958_06110"
     CDS             complement(1231..1791)
                     /locus_tag="VF958_06110"
                     /inference="COORDINATES: protein motif:HMM:NF019265.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HEX9148720.1"
                     /translation="MSDDRSLLERIRRGDTEGAEELFERYAPALLRFADRMLCDRDSA
                     EEVTQEVFVKVISRAHQYDGRAAVSSWLFAIAANACRDRRRRDRRAPVVPLDAIADLP
                     ARGEGVESRLAGRQRRDAVRRALDSLSGEQREALILARYHGLPYAEIARVLGISVGAV
                     KTRIFRAVESLKARFSEGEASWNAVN"
     gene            1895..2587
                     /locus_tag="VF958_06115"
     CDS             1895..2587
                     /locus_tag="VF958_06115"
                     /inference="COORDINATES: protein motif:HMM:NF014244.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HEX9148721.1"
                     /translation="MAFKVVDFAIQLSVILFAISFHESAHAWAALKFGDTTARDLGRI
                     SLNPLRHLDPVNSVLLPAIMYIISSGRLIFGAAKPTPVNLRNTRNPRLANLVVSAAGP
                     VSNFLLAALSLAVLLAIKRSAPKAIIDLALAIQGDRFAAGALAPFTYVLFQFTVVNVL
                     LGVFNLIPIPPLDGSGVVISVFGGAVARAYMTIAPLGFLILILLLWSGILSRVLGPVI
                     NVVYSLIFSGIA"
     gene            2584..3588
                     /gene="trpS"
                     /locus_tag="VF958_06120"
     CDS             2584..3588
                     /gene="trpS"
                     /locus_tag="VF958_06120"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015949929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HEX9148722.1"
                     /translation="MNEPVVLSGLRPTGRVHLGNYFGAVKNWVDLQNSEKYRCFFFVA
                     DLHALTTDYADTASLAAYTREAVLDWLAVGLDPQRATVFLQSRVPQHAELALYLSMIT
                     PLGWLERVPTYKDQIDALRSRDLATHGFLGYPVLMTADIAIYRANLVPVGEDQASHLE
                     ICREIVRRFNGLYGPVFPEPKALYTPTPKVNGIDARKMSKSYNNTIGVSESAEGVREK
                     VMAMVTDPARVRRQDPGNPDHCNLFPFHVLYSPPDEVAVVDRECRTAARGCVDCKKHL
                     ISNMNPALEPIRQKRADLLSRPGDVVRDVLHSGDETARRVAEETMERVREAVKLSPEP
                     "
     gene            3596..>4022
                     /locus_tag="VF958_06125"
     CDS             3596..>4022
                     /locus_tag="VF958_06125"
                     /inference="COORDINATES: protein motif:HMM:NF014654.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HEX9148723.1"
                     /translation="MTDETIERSLPPISIPAFSGPFDLLLHLIRVNEVSITDIPIAEI
                     TEQYTAYLDTMRELDLDVASEYVAMAAELIYIKSRMLLPRAPGEPEESPLEDLQRRLL
                     EYQRYKEAAETLHEVDALRAGLWSRPPTSPPPSPDGETLE"
BASE COUNT          635 a         1427 c         1295 g          665 t
ORIGIN      
        1 tgggccgcga cgacgatagt cgcgaccgct gtgaactccc ggccagggac cgcgatgacg
       61 tcgcccgaaa cgttgtcgag gcgcagggtt gcccccgacg gcaggttcgc cgagatccga
      121 tccgtccggt tcgccgtctc gtcgccgcgc gccgtgcccg ccgccagcag catcagggca
      181 gcgccgagcg cgaggagcag ccagaggacg acgcgctccg tgtgatcccc tcgcctgctc
      241 atgtcgcgcg gagtgctcat cacaaggaga gacgcccctc ctcgatcgga gttacgcgtt
      301 ctcacaggtc gatcgccttc agggcggacg cggcccgggc gcggacgaac ccgttttcgg
      361 aggggtcgaa cgccttgtcg cggagagact gaatcgtctt cgggtccggc gcctccttgg
      421 cggccgcggc gagggcgtcg accgcgacca gcctcacccc ggggttcggg tcgttcgcga
      481 gcgcatcgag gagcgcggcg cggatctggc tcgtcgagcg gaaggacgcc agcgcctccg
      541 ccgctttctt gcgcacaccg ggattgcggt cgcgcttcag ggtcgcggcg agagcgtcga
      601 cgatctccgg cgacgccacc gcggcgccag ggccgaaatt ccccgacacg agctcgatcg
      661 ctcgggattt ctctccggcg gtctcgttgc gcgagagcag atacgcgagg agactggaga
      721 cgcgcgggtc gtccggtcgc ccggtgatcg agtggtgcgt cgtcgcgtcg aaggcgatcc
      781 ccacccggcc atcggcgtcc gtcttgtagg agaggttcgt gaggctcgga ttctccgaga
      841 ggtccggcca cgagggcgga accgccgacc cggcggccgc aacgccgttg cccggcttcg
      901 gtgccgtgcc gagcccaccc ttcgcggcga ggaagccggc ggccgcagcg agaacgaccg
      961 ccgcggcctg gccgagggcc cgccacgcgc gcgaacgggg gcggaactcg cgaatgcgcc
     1021 gccgcgcgat ctcgtcctcg agaagcgcaa gcgtgcgggc ccggtactcg ggcgtgacga
     1081 ctgcttcggg gtcgcttccg agcgtctgcc acgtctcttc gacggtcgcg agttcagcgc
     1141 ggcaggactc gcagccggcg aggtgaccct ggagctcctc gcgctcgcgc gacgacgctt
     1201 ctccggtcag acgtccgatc gcgtactcgg tcagttcacg gcattccatg aggcttctcc
     1261 ttcggaaaaa cgcgccttca ggctctccac ggcgcggaag attcgtgtct tgaccgcccc
     1321 gaccgatatt ccgagaacgc gtgcgatctc ggcgtagggg aggccgtgat atcgggcgag
     1381 gatgagcgcc tcgcgctgct ccccggagag actgtcgagc gcccgccgaa cggcgtcccg
     1441 ccgctgacgt ccggccagcc tcgattccac gccctcgccc cgggccggca ggtccgcgat
     1501 ggcgtccaga gggacgacgg gcgcccggcg gtcgcggcgc ctccggtcgc ggcacgcgtt
     1561 cgccgcgatc gcgaaaagcc aggacgagac cgcggcccga ccgtcgtatt gatgcgctcg
     1621 agaaatcacc ttgacgaaaa cctcctgtgt cacctcttcc gccgaatcgc ggtcgcagag
     1681 catccggtcc gcgaaccgca ggagagccgg ggcgtagcgc tcgaagagct cctccgcacc
     1741 ctccgtgtcg ccacggcgga tgcgttcgag gaggctgcgg tcgtcgctca ctccgccggc
     1801 cacgccttca cgctcaactc gggagacgag accggggtca aaaggtttcg cggcaatcgc
     1861 taagagctga ctgaacgcga gttatattgc cgccgtggca ttcaaagtcg tcgacttcgc
     1921 catccagctg tcggtcatcc tgttcgccat ctcgtttcac gagtcggctc acgcgtgggc
     1981 agcgctcaag ttcggggaca cgaccgcccg cgacctcggg cggatcagcc tgaacccgct
     2041 gcgacacctc gatcccgtca acagcgtgct cctgccggcc atcatgtaca tcatttcctc
     2101 gggacgcctc atcttcggcg cggcaaagcc gacgccggtc aacctccgaa acacgcgcaa
     2161 cccgcgcctc gcgaatctcg tcgtctccgc cgccggtccg gtctcgaact tcctgctcgc
     2221 cgccctatcg ctcgccgtcc tcctcgcgat caaacggtcg gcgcccaaag cgatcatcga
     2281 cctggcgctc gccatccagg gcgatcggtt cgccgcgggc gctctcgcgc cgttcaccta
     2341 cgtcctcttt cagttcaccg tcgtcaacgt gctgctgggg gtgttcaacc tcatcccgat
     2401 tccgccgctc gacggaagcg gcgtggtcat ctcggtattt ggcggcgcgg tcgcgcgcgc
     2461 gtacatgacg atcgcgcccc tgggcttcct catcctgatc cttctcttgt ggagcggaat
     2521 cctctctcgg gtcctcggtc ccgtcatcaa cgtcgtgtac tcgctcatct tcagcgggat
     2581 cgcttgaacg agccggtcgt tctctccggg cttcgcccca cgggccgcgt tcacctcggc
     2641 aactacttcg gtgcggtcaa gaactgggtc gacctccaga actccgagaa gtatcggtgc
     2701 ttcttcttcg tcgccgatct gcacgccctg acgaccgact acgccgacac cgcgagcctc
     2761 gccgcgtaca cgcgggaggc ggtgctcgac tggctcgccg tcggcctcga tccgcagcgc
     2821 gcgacggtct tcctgcagtc gcgggtgcct caacatgccg agctcgcgct ctacctctcg
     2881 atgatcacac cgctcggctg gctggaaagg gtcccgacct acaaggatca gatcgacgct
     2941 ctgaggtctc gcgacctcgc aacgcacggg tttctcggat atccggtctt gatgaccgcc
     3001 gacatcgcca tctaccgggc gaacctcgtg ccggtggggg aggaccaggc gagccacctc
     3061 gagatctgcc gcgagatcgt gcgccgtttc aacggtctct atggcccggt cttccccgag
     3121 ccgaaggcgc tctacacgcc gacgccgaag gtgaacggaa tcgacgcacg caagatgtcg
     3181 aagtcgtaca acaacaccat cggagtctcc gagtcggccg agggagtgcg cgaaaaggtc
     3241 atggcgatgg tgacggaccc ggcgcgcgtc cggcggcagg acccgggcaa cccggatcac
     3301 tgcaatcttt ttccgttcca cgtcctctac tcgccgcccg atgaggtcgc ggtcgtcgac
     3361 cgggagtgcc gcaccgccgc ccgcggctgc gtcgactgca agaagcacct gatctccaac
     3421 atgaacccgg ccctggagcc gatccggcag aagagggccg acctcctttc ccggccggga
     3481 gacgtcgtgc gggacgttct ccactcgggg gacgagaccg cgcgtcgcgt cgccgaagag
     3541 acgatggagc gggtgcgaga ggcggtcaag ctgagtcccg agccgtgagc cgtccatgac
     3601 cgacgaaacc atcgagcgct cgctgccgcc gatcagcatc cccgcgtttt cgggtccctt
     3661 cgacctcctc ctgcacctga tccgagtgaa cgaggtctcg atcaccgaca tcccgatcgc
     3721 cgagatcacc gagcagtaca ccgcctacct cgacacgatg cgcgagctcg acctcgacgt
     3781 cgcctccgag tacgtcgcga tggcggcgga actgatctac atcaagtcga gaatgctcct
     3841 gccgcgcgcg ccgggggagc ccgaggagag cccgctcgaa gaccttcagc gtcggctgct
     3901 cgaatatcag cgctacaagg aggcggccga gacgctgcac gaggtcgacg ccctgcgggc
     3961 cgggctctgg tcccggccgc cgacctcgcc gccgccatcg cccgatggtg agacgctcga
     4021 gg
//
LOCUS       DASWAV010000296         1751 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U2665
            k141_373951, whole genome shotgun sequence.
ACCESSION   DASWAV010000296 DASWAV010000000
VERSION     DASWAV010000296.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960316
            Sequence Read Archive: SRR10260007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 1751)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1751)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:47:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,929
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1751
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_373951"
                     /isolate="SMAG_U2665"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.36444 N 132.31889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(257..1432)
                     /locus_tag="VF958_06130"
     CDS             complement(257..1432)
                     /locus_tag="VF958_06130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019987952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6600 domain-containing protein"
                     /protein_id="HEX9148724.1"
                     /translation="MKRLSITTLILAALAWGFSAQPARAQISVSISIGGFYDELEPYG
                     RWVDCNYGGYGDCWVPSRVSRGWQPYSNGEWVYTEYGWTWVSYDEWGDDPYHYGSWVY
                     TNRYGWAWIPGTVWAPAWVTWSYSDNYVGWAPLPPSIVFGSSGYSGRPIRMSPAQYVF
                     VPTNRFIGSNIASVRIAPQQSATIFRQTTPITRFGVSGGIIRNTAIPIETIERATRNR
                     IPRRDIRDAKTAPRPVTEWTRGNQRQVSIVAPTREVRDAIASRPQGRTNRRPEAVQQP
                     AAPRGESPRRQEPRGRAQQVPPAAPPVQVRKAPPPAQPQPAPEVYRGKPETSNAPANR
                     GRKAAPARPAQAAPPPQAAPPAVQAPPGQVQKQQPGRGKPEKAQKGEKGKKEKPEKD"
BASE COUNT          263 a          605 c          568 g          315 t
ORIGIN      
        1 taggtcactc tgccgaacag gagccgcccg ccaccactcg cgtttccagc aacgaactcg
       61 ctccactcgt gatccccgct gtccttgtgg gcccagctga tgtcgccgtt cgtgcctgca
      121 aagtaaccat cgagcgtcac ctgattgaaa acgacgagcc tcggcatgtc ctcccctcac
      181 cctgaccctc tccccgcgcg cggggagagg ggagagtcga cagtcggcag tgattcggcg
      241 cggcgaagtt cgggtctcag tccttctcgg gcttttcctt cttgcccttt tcgcccttct
      301 gcgctttttc cggctttccg cgtccaggct gctgcttctg aacctggccg ggaggcgcct
      361 gaacggcggg cggagcggcc tgcggcggcg gagcggcctg agcgggtcga gcgggagcgg
      421 ctttccggcc gcgatttgcc ggcgcgttcg aagtttccgg cttgccgcgg taaacctccg
      481 gcgccggctg gggctgcgcg gggggcggag ctttccggac ctgaacggga ggcgctgcgg
      541 gcgggacctg ttgggcccgg cctcgcggtt cctggcgccg cggtgactcg ccgcggggag
      601 ccgccggctg ttggaccgcc tcggggcggc ggttggttct tccctgcggc cgcgaggcga
      661 tcgcgtccct cacctcgcgg gtgggagcga cgatcgacac ttgccgctgg ttgccgcgcg
      721 tccactcggt caccggtcgc ggcgcggttt tcgcgtcgcg gatgtctcgc ctcgggattc
      781 gattgcgggt cgcacgctcg atcgtctcga tcgggatggc cgtgttccgg atgatgccgc
      841 ccgaaactcc aaaacgcgtg atcggcgtcg tctgacggaa gatcgtcgcg ctctgctgtg
      901 gcgcaattcg caccgacgcg atgttcgagc cgatgaaacg attcgtgggc acgaagacgt
      961 actgtgccgg gctcatgcgg atcggccgcc cggagtaccc cgacgagccg aagacgatgc
     1021 tcggcggcag cggcgcccag ccaacgtagt tgtcgctgta gctccacgtc acccacgccg
     1081 gtgcccagac ggtgccggga atccacgccc atccgtaccg gttcgtgtag acccacgacc
     1141 cgtagtggta ggggtcatct ccccactcgt cgtaggacac ccacgtccac ccgtattcgg
     1201 tgtagaccca ctcgccgttc gagtacggct gccagccgcg ggagacccgc gagggcaccc
     1261 agcagtcgcc gtagcccccg taattgcagt cgacccaccg gccgtacggt tccagctcgt
     1321 cgtagaaccc gccgatcgag atgctcaccg agatctgagc tcgcgccggc tgcgctgaga
     1381 aaccccaggc gagcgccgcg agaatcaacg tcgtgatcga gagtctcttc atgttgtcgc
     1441 tccttagcta cggcccctcc gtgttccgca cgacccacgc catcggagac gggattgatc
     1501 tcgcccgacg aaacggaaag tacggggtcc ggtctccact gaccactccg ggaagcaagc
     1561 gggacaggga ggccggaccc cgaccaggga cgcttcgttt acggtcgccg atcccgattc
     1621 cgatcccgcc ggtgctcatc tctccagcgc cggtaatcgt cgccctcgac cacgcgctga
     1681 ttcgaccagt ggcccggaac gtagcgatag ccaccgtatt cgcgaacgta gcgcgggccg
     1741 acccagcgaa c
//
LOCUS       DASWAV010000297         4650 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U2665
            k141_736149, whole genome shotgun sequence.
ACCESSION   DASWAV010000297 DASWAV010000000
VERSION     DASWAV010000297.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960316
            Sequence Read Archive: SRR10260007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 4650)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4650)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:47:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,929
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4650
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_736149"
                     /isolate="SMAG_U2665"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.36444 N 132.31889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(86..2494)
                     /locus_tag="VF958_06135"
     CDS             complement(86..2494)
                     /locus_tag="VF958_06135"
                     /inference="COORDINATES: protein motif:HMM:NF021126.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhO family protein"
                     /protein_id="HEX9148725.1"
                     /translation="MFLLVVRLLAVYFATCAVAFFLVNRFVTKVRPGIAILLSFGPFL
                     LTGKAMVTAGVYAPLDIAYQAPPLAAHREELGIRGTRSPILGDVVTQFIPFRKAVRDA
                     VKNGRLPLWNRFALAGEPLLANTQPAVLHPGTLIGFLLPLAQAWTFEMSLRYFLALLS
                     MYLFLRDLGCREMPSLLGAVGWCFSDYLVFFAGWQHSAAAPPIPFLLLALRRLVREPG
                     DRSVALTVVALLLVVTAGHPETLLHGVAAAGVYFLFELVFAGPGRRLRPLLLSLVAGA
                     LALGLSAVLLLPLVEALPHTAEHFFRSGIYAHLDKSVSVAESLRRSIPDVVPYAFGVS
                     GHGRSAIGFGEPAAYAGAVVLPFAIVGLFSRRREKWPLLLIAFVGLAMWARLPVVTDA
                     IASLPLFDIGLNERMVFLGALGISALAGLGAERFLERDGISLLSATLATVAVVLLLHR
                     EVRPTLIRLEMDPAFLRDRLLAEVIPLVLLAIVAAISLPRKRTTPVLFSALVILLLQR
                     RLEAGDLYPTFPNRAFYPDLAVLDPIPRHEPWRFTATGYQFIPNIAALYELEDVRGYE
                     AMTFLPLTWTFPLWCVPQPIWFNRVDDPTTPFLAFLNVRWVLASADWTPPRGWKALAE
                     GDGTRLFENPRALPRAFVPRMLRAFPDPEQREALGSIGDFAERGVVDDAGPDWPVGEW
                     RANGVATVRIDSYQPQRLDLAVDARSKAVVGTSIPRWPGWRLEIDGVRAPLFTYNAAF
                     LGFAVPAGSRRAVLRYLPDAFLAGYAISAATAAVCVVTIAARRRRPARARSAPREPGA
                     PRSA"
     gene            complement(2501..>4650)
                     /locus_tag="VF958_06140"
     CDS             complement(2501..>4650)
                     /locus_tag="VF958_06140"
                     /inference="COORDINATES: protein motif:HMM:NF021126.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="YfhO family protein"
                     /protein_id="HEX9148726.1"
                     /translation="AAAENDVDRIHNPILSDLAFANLPWRAAVREAFVNGRLPLWNRF
                     VLGGNPLLGTAQSGVFHPSTWFGIFLPLALSWTFSCAFTIFLALVSGYLFFREFCRSD
                     LGALTGAVGWGFSTYVLFWGGWAVGPSIATFPLLLLGLRRLARGGERGITLASTALIL
                     SVAGGHPETLFHSIAAGGVYFVSELFASDARRRAGRAIAGSLGAGLLAACLAAPVLLP
                     LLEAVPHSAEYRERRAALAKGRSGQSVPADEAARRILPVVLPFAHGIYGKSPVQDVRQ
                     DGSGMPLAYAGAVLFPLAALGLLSRAVARSRWIFLVFILLGVAYGASAPLLLDLTSRL
                     PGFALALNYRLVFLAPLGLAGLAALGADRLAESRQSLGVAATAVAAALMFAYVLAVPV
                     FRERGLTAGFTRPSMAFEVVPVVLLLVASFSLRRRLAAAALLLLVGQRFFEMRGTYPT
                     LPAGTLAPSLSIFSVLPRTADPYRIVAAGETFRPNAAALYGLEDVRGYESLVLDRFAE
                     TYPLWCRPQFASFNRVEDLDPPFLSFVNVRYAIAELTSAAPPGWVLVARTREGSVFEN
                     PRVQPRAFVPRRLRVQPDAKLRLAEMGSAPSLAEVAWLGRPPGEGVNGSAQLAVREIG
                     TDLIVEADVRDRALVATSIPDWPGWGAKDGAADVPLVTVNHAFVGFWLAPGRHVVRLH
                     YLPSSFLLGSLVAGGAVIAVSIAAVLRRRRGLR"
BASE COUNT          912 a         1529 c         1670 g          539 t
ORIGIN      
        1 ctcctcgccg gcttcctggt attcacgtac ttgacgacgg aggcggctcc ggcgctcctg
       61 atcgcctggt ccgcggcgcg ggttttcacg ccgatcgcgg cgctcctggc tctcgcggcg
      121 cactcctcgc gcgcgccggc cggcggcgtc gcgccgcgat agtgacgacg cagaccgctg
      181 ctgtcgccgc ggagatcgcg tagcccgcga gaaaagcgtc gggcaggtag cgcagcacgg
      241 cgcgccggga tcccgctggg accgcgaatc cgaggaacgc ggcgttatag gtgaagagag
      301 gtgcgcgcac accgtcgatc tcgagcctcc acccgggcca gcgtgggatc gaggttccga
      361 cgacggcctt cgatcgagcg tctactgcga gatcgagacg ttgcggctgg tacgagtcga
      421 tccgtaccgt cgcgactccg tttgcgcgcc actcgccgac gggccagtcg gggccggcgt
      481 cgtccacgac gccccgttcg gcgaagtccc cgatcgaccc gagcgcctcg cgctgctccg
      541 ggtccggaaa tgcgcgaagc atccgcggga caaacgcgcg cggcagcgcc cgagggttct
      601 cgaagagccg ggttccatcc ccttccgcga gcgccttcca gccgcgcggc ggcgtccaat
      661 cggcggacgc gagcacccac cgcacgttca ggaacgcgag aaacggagtc gtcggatcgt
      721 ctacgcggtt gaaccagatg ggctgcggaa cgcaccacaa cggaaaggtc caggtgagcg
      781 gcagaaatgt catcgcctcg tagccacgca cgtcctcgag ctcgtagagc gcggcgatgt
      841 tcgggatgaa ctggtagccc gtcgccgtaa agcgccacgg ttcgtgccgg ggaatgggat
      901 cgagcaccgc gaggtccggg taaaacgccc gattcgggaa agtcgggtac agatcgcccg
      961 cttcgagcct tcgttgcaga aggaggatca cgagggccga aaagagcacc ggcgtcgtac
     1021 gcttccgcgg cagcgagatc gccgcgacga tcgcgagcag aaccagcggg ataacctcgg
     1081 ccagcagccg gtccctcagg aacgcgggat ccatctcgag tcggatcagt gtcggccgaa
     1141 cctcccggtg cagcagaaga accacggcca cggtcgcgag agtcgcggac agcaacgaga
     1201 tgccgtccct ctcgaggaac cgctcggccc cgaggcctgc gagtgcggag atccccaacg
     1261 cgccgaggaa caccatccgc tcgttcaacc cgatgtcgaa aagcggaagc gacgcgatcg
     1321 cgtcggtgac gaccggaagg cgtgcccaca tggcgaggcc cacgaacgcg atcaagagga
     1381 gcggccactt ttcgcgccgc cgcgagaaaa gaccgacgat cgcgaacggg agcaccaccg
     1441 cgccggcgta cgctgccggc tcgccgaaac cgatcgccga ccggccgtgc cccgagactc
     1501 cgaaggcgta cgggacgacg tccggaatgg agcgccgcag gctctcggcg acgctgacgg
     1561 acttgtccag gtgcgcatag atgccggatc ggaagaagtg ctcggcggta tgcggaaggg
     1621 cttcgacgag cggcaggagc aggaccgccg aaagcccgag agcgagcgct ccggcgacga
     1681 gcgagagcag gagcgggcgc agcctccgtc ccgggccggc gaacacgagt tcgaagagga
     1741 aatacacgcc ggcggcggcc acgccgtgga ggagcgtttc cgggtgcccg gccgtgacga
     1801 cgagaaggag agcaacgact gtcagggcga cggatcgatc gcccggctcc cgcacgagcc
     1861 gcctcaacgc gagcaggaga aagggtatcg ggggcgctgc cgcggagtgc tgccagcctg
     1921 cgaagaaaac gaggtagtcg gagaagcacc atccgacggc gcccaggagc gaaggcatct
     1981 cccggcagcc gagatcgcgc aggaagaggt acatcgagag cagtgcgagg aagtagcgca
     2041 gggacatctc gaacgtccag gcctgcgcga gaggaaggag gaagccgatg agcgtcccgg
     2101 ggtgcaggac cgccggctgt gtgttcgcga ggaggggctc gcccgcgagc gcgaagcggt
     2161 tccagagggg aaggcgcccg ttcttgaccg catcgcgcac ggccttccgg aaaggaatga
     2221 actgggtgac gacgtcgccc aggatcggag agcgcgtccc ccggatgccg agctcctccc
     2281 gatgggccgc gaggggcggc gcctggtatg cgatgtcgag cggcgcgtag acgccggcgg
     2341 tcaccatagc cttccccgtg agaagaaagg gtccgaacga aaggagaatc gcgattcccg
     2401 gccggacctt cgtgacgaac cggttcacga gaaaaaaggc gaccgcgcag gtcgcgaagt
     2461 agacggcgag gaggcggacg acgaggagaa acaagcgcgg ctagcgtaac ccgcgccggc
     2521 ggcgaaggac cgcggcgatc gagacggcga tcacggcccc ccccgcgacg agcgatccga
     2581 gaagaaacga cgacggcagg tagtgcaagc gcacgacgtg tcgccccggg gcgagccaga
     2641 agccgacgaa ggcgtgattc acggtcacga gagggacgtc ggcggcgccg tccttcgccc
     2701 cccagcccgg ccagtcggga atcgacgtgg cgacgagcgc gcggtcgcgg acgtcagcct
     2761 cgacgatcag gtccgtcccg atctcgcgca cggccagctg cgcgctcccg ttcactcctt
     2821 ctcccggggg ccgaccgagc cacgcaacct cggcaaggct cggtgcgctt cccatctccg
     2881 ccagccgcag tttcgcgtcc ggctgtacgc gcagccgccg gggcacaaac gcgcggggct
     2941 gtacgcgggg gttttcgaaa acggagcctt cgcgtgtccg tgcgacgagc acccaaccgg
     3001 ggggcgccgc agacgtgagt tccgcgatcg catagcggac gttgacgaac gacaggaacg
     3061 gggggtccag gtcctcaacg cggttgaacg acgcgaactg aggacggcac cacagcggat
     3121 acgtctcggc gaaccggtcg agcacgagcg attcgtagcc gcgcacgtcc tcaaggccgt
     3181 agagcgccgc ggcgttcggg cggaacgtct cgcccgcggc gacgatccgg tacggatccg
     3241 cggtcctcgg cagcaccgag aaaatcgaga gggacggcgc gagcgtgccc gcgggcaagg
     3301 tcgggtaggt gccacgcatc tcgaagaagc gctgaccgac gagaaggagg agcgccgctg
     3361 cggcgaggcg tcgccgaagc gagaacgacg ccacgagcag gagaacgacg ggcacgactt
     3421 cgaacgccat cgagggtcgc gtgaacccgg ccgtgaggcc gcgctcgcgg aagacgggca
     3481 ccgcgaggac ataagcaaac atcagcgccg cggctacggc ggtcgccgca acgccgagcg
     3541 attgtctcga ttcggcgagg cggtcggcgc cgagcgcggc cagcccggcg aggccgagcg
     3601 gcgccaggaa gaccagccga tagttgagtg cgagcgcaaa tccgggaagg cgcgacgtca
     3661 ggtcgaggag caacggcgcg ctcgcgccgt acgcgacgcc caggagaatg aaaaccagaa
     3721 agatccaacg cgagcgagca acggcgcgcg acaggaggcc cagcgcagcg agaggaaaca
     3781 gaaccgcccc ggcgtaagcg agcggcattc ccgatccgtc ctgccgcacg tcctggaccg
     3841 ggctcttccc gtagatcccg tgcgcgaacg gcaggacgac gggaaggatt cgccgcgccg
     3901 cttcgtcggc cgggaccgac tgcccgctgc gtccctttgc gagcgccgcc cgcctctccc
     3961 ggtactcggc cgagtgcggg acggcttcga ggagcgggag cagcaccggc gccgcgaggc
     4021 acgccgcgag gagtcccgcc ccgagcgatc ccgcgatcgc cctcccggcg cgccggcgag
     4081 cgtccgacgc gaagagctcc gacacgaaat agacgccgcc cgccgcgatc gagtgaaaca
     4141 gtgtttccgg atgtcctccg gcgacggaga ggatgagcgc cgtcgacgcg agcgtaatcc
     4201 cccgttctcc gccgcgcgcg agccgtcgca gtccgaggag aaggagcggg aaggtcgcga
     4261 tcgacggccc cacggcccag ccgccccaga agaggacgta ggtcgagaaa ccccatccga
     4321 ccgcgccggt gagcgctccg aggtcggacc gacagaattc tcgaaagaag aggtagcccg
     4381 agacgagcgc caggaagatc gtgaacgcgc aggagaaggt ccacgacagc gcgagcggca
     4441 gaaagatccc gaaccacgtc gaggggtgga agactcccga ctgcgccgtc ccgagcagag
     4501 ggttccctcc gaggacgaag cggttccaga gcggcagccg tccgttgacg aaggcctcgc
     4561 gtaccgccgc ccgccacggc aggttcgcga acgcgaggtc cgagaggatg gggttgtgga
     4621 tgcggtccac gtcgttctcc gcggcggccg
//
LOCUS       DASWAV010000298         2630 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U2665
            k141_456879, whole genome shotgun sequence.
ACCESSION   DASWAV010000298 DASWAV010000000
VERSION     DASWAV010000298.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960316
            Sequence Read Archive: SRR10260007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2630)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2630)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:47:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,929
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2630
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_456879"
                     /isolate="SMAG_U2665"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.36444 N 132.31889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(27..566)
                     /gene="atpH"
                     /locus_tag="VF958_06145"
     CDS             complement(27..566)
                     /gene="atpH"
                     /locus_tag="VF958_06145"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF012439.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit delta"
                     /protein_id="HEX9148727.1"
                     /translation="MSRFARPYARAFLDSTPASFDVESFLERAAAVARATAADRRLRN
                     FFAAPSVPIDTKRRALEDLARSVGIDDFGRRFLEMVLENRRWLALDEILSAVREARDR
                     QLGVVEASVTVAAPVGDEERKRIENALAKQLGRSVRMNIRVDSAILAGFVARVGSEVF
                     DASVVRAIERFHGDAAGAR"
     gene            complement(563..1141)
                     /locus_tag="VF958_06150"
     CDS             complement(563..1141)
                     /locus_tag="VF958_06150"
                     /inference="COORDINATES: protein motif:HMM:NF012645.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9148728.1"
                     /translation="MISGGRLFAAIPLSLLAQEAEEHASTFLGLPLWLWQLVNLVAFL
                     GVLLYFVARPLAAMFRQRQLAVEERLREAKELRAEAARLGAEVHERMARLDQEIAEVR
                     ARGLAEGEAERVALSEKADREVERVRREAEEEIGRRLAAAKQDLRRVAADLTSATARE
                     MLVSQITDDDRRRLLEEGVSRLAEQRPEGEAR"
     gene            complement(1138..1572)
                     /locus_tag="VF958_06155"
     CDS             complement(1138..1572)
                     /locus_tag="VF958_06155"
                     /inference="COORDINATES: protein motif:HMM:NF012645.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit B"
                     /protein_id="HEX9148729.1"
                     /translation="MIKAPDVTLLYVMLAFVVSYAVMKRFLFRPLGAILEQREEEERA
                     SARIHADSIRELERAVAEGEARLSLARREALKEREALRAQGLAKFEQDLGRAREAASS
                     SVQAASQTIDADAQSAAAELPSRSRALAVELAEKILGRKLAA"
     gene            complement(1781..2179)
                     /locus_tag="VF958_06160"
     CDS             complement(1781..2179)
                     /locus_tag="VF958_06160"
                     /inference="COORDINATES: protein motif:HMM:NF016408.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymer-forming cytoskeletal protein"
                     /protein_id="HEX9148730.1"
                     /translation="MKGTTPEGTLNGFLDRGSHFSGDLKFEEGFRIDGRFEGKITSGS
                     ELVLGETAEVDAEIQVAKLSVNGSLKGTITATERIELHAKARVLADLTTPALSIDEGA
                     FFQGSCKMGHETPSNVIGMPATAGAERHHS"
     gene            complement(2235..>2630)
                     /locus_tag="VF958_06165"
     CDS             complement(2235..>2630)
                     /locus_tag="VF958_06165"
                     /inference="COORDINATES: protein motif:HMM:NF036800.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partitioning protein ParB"
                     /protein_id="HEX9148731.1"
                     /translation="KDRTTVTNALRLLKLPESVRGRIRSGLLSAGHARALVSLVSADD
                     QERLAEEILRRGLSVRQTEKRVASFGSADKLRKERRRDPFTHDAEEKLSRRLRARVRI
                     VRRRRGGRVEIAFASEDELIGLFEKLSRA"
BASE COUNT          418 a          929 c          829 g          454 t
ORIGIN      
        1 tcaggaaact gcgatcgatt ccgccgtcag cgggcgcccg ccgcgtcgcc gtggaaccgc
       61 tcgatcgcgc ggacgacgga ggcgtcgaag acttcgctcc cgacgcgcgc gacgaatccg
      121 gcgagtatcg ccgaatccac ccgaatgttc atccgcacgc tacgccccag ctgcttcgcg
      181 agcgcattct cgatccgctt gcgctcctcg tctccgacgg gtgcggcgac ggtcaccgac
      241 gcttcgacga ccccaagctg gcggtcgcga gcctcccgga ccgcggagag gatctcgtcg
      301 agcgcgagcc atcggcgatt ctccaggacc atctccagga agcgccgacc gaaatcgtcg
      361 atgccgacgc tccgcgccag gtcttccagg gcgcggcgtt tcgtgtcgat cggaaccgaa
      421 ggcgctgcga agaagtttct caggcgtcgg tcggccgcgg tcgcccgtgc gacagcggcc
      481 gcgcgttcga gaaagctttc cacgtcgaag ctcgccggcg tcgagtcgag aaacgcccgc
      541 gcataagggc gcgcgaaccg actcatcgag cctccccttc gggccgctgc tccgccagcc
      601 tcgagacccc ttcctcgagg agacggcgcc ggtcgtcgtc ggtgatctgc gacacgagca
      661 tctcgcgggc cgtcgcgctc gtgaggtcgg cggcgacgcg tcgaagatcc tgcttcgccg
      721 cggcgagacg tcggccaatt tcctcttccg cttcccgcct cacgcgctcg acctcacgat
      781 ccgctttctc cgagagcgcc acccgctccg cctcgccctc cgccagaccg cgggcccgga
      841 cttcggcgat ctcctggtcg agacgcgcca ttctctcgtg gacctcggcg ccgaggcgcg
      901 ccgcctccgc ccgcaactcc ttcgcttccc ggagccgctc ctcgaccgcg agctgccgct
      961 ggcggaacat cgcggcgagc ggacgcgcga cgaagtagag cagcaccccg agaaacgcga
     1021 cgaggttgac cagctgccac agccacagcg gcaggccaag aaaagtggag gcgtgctctt
     1081 ccgcctcctg cgcgaggagg gacaggggaa tcgcggcgaa cagtcgcccg ccggaaatca
     1141 cgcggcgagc ttccgtccga gaattttttc cgcgagctcg accgcgaggg cgcgcgagcg
     1201 cgatgggagc tcggcggccg cgctctgtgc gtccgcgtcg atcgtctggc tcgccgcctg
     1261 gaccgacgac gacgcggctt cccgcgcccg accgagatcc tgctcgaact tcgccaggcc
     1321 ctgcgcccgc agcgcctcgc gctccttcaa ggcctccctc cgagcgagcg acagccgcgc
     1381 ttcgccttcc gcgacggccc gctcgagctc gcggatgctg tcggcgtgaa tacgcgcgga
     1441 cgcgcgctcc tcctcctcgc gctgttcgag aatggcgccg agcggccgga agagaaacct
     1501 cttcatcacc gcgtacgaca cgacgaacgc gagcatcacg tagaggagcg tcacatcggg
     1561 cgctttgatc atggctgtgc cgcgctattc cttgcgattt cgtgaatctt ggagcgagag
     1621 gacggcccgc tacgccccgg gacggaacgc ggcggatgaa tagcacacgg cggcgaggaa
     1681 accaagacct cgacgtctcg aatccgaccg gccggcgttc ctcccggcgt gaactccaat
     1741 gccgggggag tctatcatta acctatttat tatcagtaac ttagctgtga tgtcgctccg
     1801 cccctgccgt cgcgggcatc ccgatgacgt tcgaaggggt ttcgtgaccc atcttgcacg
     1861 acccctggaa gaatgctcct tcgtcgattg aaagggccgg cgtagtcagg tccgcgagaa
     1921 cacgcgcctt ggcgtggagt tcgatccgct ccgtcgcggt gatggttccc ttgagcgagc
     1981 cgttcaccga cagcttcgcc acctgaatct cggcgtcgac ctcggcggtt tcaccgagca
     2041 cgagctcgct tcccgacgtg atcttcccct cgaacctgcc gtcgatccgg aatccctcct
     2101 cgaacttgag gtctccggag aagtggcttc cgcggtccag aaaaccattc agcgttcctt
     2161 ccggggtcgt gcctttcatc gcgccttcct tcctggttcg gtccctgggt tgatggtgga
     2221 ttcggaaccc atgattatgc ccgcgaaagc ttctcgaaga gcccgatcaa ttcgtcctcc
     2281 gacgcgaatg cgatctcgac cctccctccg cgcctccgcc ggacgatccg gacccgcgct
     2341 cgcagccggc gggagagctt ctcctcggcg tcgtgcgtga acggatcgcg acggcgctct
     2401 tttcgaagct tatcggcgga tccgaagctg gcaacgcgtt tttcggtctg gcgcacggag
     2461 agcccccggc ggaggatttc ttcggcgagc cgctcctggt cgtcggcgga gacgagagac
     2521 acgagggcgc gggcatgtcc ggcgctcaga agaccggagc ggatccgccc gcggacgctc
     2581 tcggggagct tcagcaggcg aagggcgttg gtcacggtgg tgcggtcctt
//
LOCUS       DASWAV010000299         2908 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U2665
            k141_496609, whole genome shotgun sequence.
ACCESSION   DASWAV010000299 DASWAV010000000
VERSION     DASWAV010000299.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960316
            Sequence Read Archive: SRR10260007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2908)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2908)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:47:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,929
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2908
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_496609"
                     /isolate="SMAG_U2665"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.36444 N 132.31889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..380
                     /locus_tag="VF958_06170"
     CDS             <1..380
                     /locus_tag="VF958_06170"
                     /inference="COORDINATES: protein motif:HMM:NF014818.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9148732.1"
                     /translation="ELLPQLHGKITGASVAVPVRNGSCVDLVTWHLRPVTKTAVNEVL
                     RTAASTDRWHHVLRYESDPIVSSDVARSWWSSTFDSLATMTLGDKVSKTVSWYDSGFG
                     YAHRAIDLVDRLARLDRAAESAA"
     gene            377..1384
                     /gene="gap"
                     /locus_tag="VF958_06175"
     CDS             377..1384
                     /gene="gap"
                     /locus_tag="VF958_06175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010098442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0019682 - glyceraldehyde-3-phosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HEX9148733.1"
                     /translation="MTIRVGINGFGRIGRSVFRILSGRDDMEVICVNDLYDNEQLAYL
                     LKYDTVMRVFQREVTADADAMYVDGRKVVMTEEKDPAKIPWGKLGVEIVVEATGVLTK
                     REKLEPHLAAGAKKVILTVPPKDKVDAMVVVGVNDGDLTSAHRIVSNASCTTNCLAPL
                     AMVLDDAFGIEEGFMTTVHAYTNDQRLADVPHKDLRRSRAAAENIIPTTTGAARAVGE
                     VLPKLKGRLDGMAMRVPVPDGSVVDLSCRLRGKPAARDVNDVVRAAAEGRLKGVLEYS
                     EVPLVSSDIIGNPHSSIFDALSTSAKGDGFVKVLSWYDNEWGYSNRVVDLIARLAELG
                     S"
     gene            1389..1757
                     /locus_tag="VF958_06180"
     CDS             1389..1757
                     /locus_tag="VF958_06180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011140042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HEX9148734.1"
                     /translation="MKHSHGFLDIVNDAKKRVRETTPEDVRRRQERGERFHLVDVRED
                     NEWERGRALGAMHLGKGIIERDIEQVIPDHDMEIVLYCGGGYRSALAADVLQRMGYRN
                     VLSMDGGWKRWNELGYPTEQ"
     gene            complement(1761..2213)
                     /locus_tag="VF958_06185"
     CDS             complement(1761..2213)
                     /locus_tag="VF958_06185"
                     /inference="COORDINATES: protein motif:HMM:NF014825.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SEC-C metal-binding domain-containing protein"
                     /protein_id="HEX9148735.1"
                     /translation="MARVSRNDPCPCGSGLKYKRCHEGKDVEKPRTSGMLLIAILALL
                     IGAGAVAVVIGISRGPDTASSSRRRVWSPEHGHWHEEGMPVRSSRGLPLDPRSALPNG
                     PRPAPPGTPPPGKVWSPEHGHWHDVVETGGSKNSPPPIGITYGPAPNR"
     gene            complement(2221..>2908)
                     /locus_tag="VF958_06190"
     CDS             complement(2221..>2908)
                     /locus_tag="VF958_06190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012236086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="quinone oxidoreductase"
                     /protein_id="HEX9148736.1"
                     /translation="LVAIPAARVVPVPDGVTTRQAAAVMLQGITAHYLSTSTYALRPG
                     DTCLVHAAAGGVGLLLCQMAKLRGARVFGTVSTDEKARLAREAGADQTIHYRTEDFVA
                     EVRRLTDGAGVQVVYDGVGRDTFEGSLDSLAVRGTMVLFGQASGAVAPIDPQVLNRKG
                     SLYLTRPTIGHYTRTREELLARSSEVLGWVGAGKLSVRIFRELPLAEASEAHRLLESR
                     RTTGKLLLIP"
BASE COUNT          486 a          977 c          986 g          459 t
ORIGIN      
        1 tggagctcct gccgcagctc cacggaaaga tcaccggcgc gtcggtcgcc gttcccgtgc
       61 gaaatggctc ttgcgtcgac ctcgtcacgt ggcacttgcg tccggtcacg aagacggctg
      121 tgaacgaggt cctgaggacc gccgcttcga ccgaccgctg gcaccacgtt ctccgttacg
      181 agtccgaccc gatcgtgtcc tccgacgtcg cccgatcgtg gtggtcgagc accttcgatt
      241 cgctggcgac catgacactc ggcgacaagg tctcgaagac ggtttcctgg tacgactcgg
      301 gcttcggcta tgcgcaccgg gcgatcgacc tcgtcgatcg tctggcgagg ctcgatcgcg
      361 cggcggagag cgcggcgtga cgatccgtgt cggcatcaac ggcttcggcc gcattggccg
      421 ctcggtgttc cgcatcctgt cgggccgcga cgacatggag gtcatctgcg tcaacgacct
      481 ctacgacaac gaacagctgg cctacctgct gaagtacgac accgtgatgc gcgtcttcca
      541 gagagaggtc acggccgacg cggacgcgat gtacgtggac ggccgcaagg tcgtgatgac
      601 cgaggagaag gacccggcga agatcccgtg gggcaagctc ggcgtcgaga tcgtcgtcga
      661 ggccaccggg gtgctgacca agcgagagaa gctcgagccc cacctcgcgg ccggcgcgaa
      721 gaaggtcatc ctcacggtcc cgccgaagga caaggtcgac gcgatggtcg tcgtcggcgt
      781 caacgacgga gacctcacga gcgcgcaccg catcgtctcg aatgcgtcct gtacgaccaa
      841 ctgtctcgcg cctctcgcga tggtcctcga cgacgccttc ggcatcgagg aaggcttcat
      901 gaccaccgtc catgcgtaca cgaacgacca gcgcctcgcc gacgttccgc acaaggacct
      961 gcgccgcagc cgcgccgcgg ccgaaaacat catcccgacg acgacgggcg ccgcgcgagc
     1021 cgtcggcgag gtcctgccga agctgaaggg ccggctcgac gggatggcga tgcgggtgcc
     1081 ggtgccggat ggctcggtcg tggacctctc ctgtcggctg cgcgggaagc cggccgcgcg
     1141 cgacgtgaac gatgtcgttc gcgccgctgc ggagggccgt ctgaaaggag tgctcgagta
     1201 cagcgaggtc ccgctggtct cgagcgacat catcggcaac ccgcactctt cgatcttcga
     1261 cgccctgtcg acttccgcaa agggagacgg gttcgtcaag gtcctgtcgt ggtacgacaa
     1321 cgaatgggga tactccaacc gagtggtgga cctgattgcg cggctggcgg agctcgggag
     1381 ctaggccggt gaagcactct catggttttc tcgacatcgt caacgacgcg aagaaacgcg
     1441 tccgcgagac gacgcccgag gacgtccggc gccgtcagga gcgcggcgag cgtttccatc
     1501 tcgtcgacgt tcgcgaagac aacgaatggg agcgcgggcg cgcgctcggc gcgatgcacc
     1561 tcggcaaggg gatcatcgag cgcgacatcg agcaagtcat tccggaccac gacatggaga
     1621 tcgttctcta ctgcggcggc ggctatcgct cggcgctggc cgccgacgtt ctccagagga
     1681 tgggttacag gaacgtgctc tcgatggacg gcggctggaa gcgctggaac gagctcgggt
     1741 acccgaccga acagtagggc ttatcggttc ggcgccggtc cgtaggtgat cccgatcggc
     1801 ggcggcgagt ttttcgagcc tcccgtctcg acgacgtcgt gccagtgccc gtgctccggc
     1861 gaccagacct ttccgggggg cggcgttccc ggcggcgcgg gacggggacc gttcggaagc
     1921 gcggatcgcg gatcgagcgg aaggccccgc gaggaccgca ccggcattcc ctcttcgtgc
     1981 cagtgcccgt gctccggcga ccagactcgc cgcctcgacg acgacgccgt gtccggtccg
     2041 cgcgagatac cgatgacgac cgcgacggcg ccggccccga tcaggagggc gaggatcgcg
     2101 atgagcagca tccccgaggt gcgcggcttt tcgacgtcct tcccctcatg acaacgcttg
     2161 tacttcaggc cgctgccgca cgggcaagga tcgtttcggg agacgcgcgc cacggtggga
     2221 ctaaggtatc agcaggagtt tcccggtcgt ccgccgcgac tcgagcaggc gatgcgcctc
     2281 gctcgcctcg gcaagcggca gctcccggaa gatccgcacg gagagcttcc ccgccccgac
     2341 ccatcccagg acttccgacg accgcgcgag gagctcctcc cgcgtcctcg tgtagtggcc
     2401 gatcgtcggg cgcgtgaggt acagcgagcc cttcctgttc aagacctgcg gatcgatcgg
     2461 agcaaccgcg ccgctcgcct gcccgaagag gaccatggtg ccgcgtaccg cgagcgagtc
     2521 gaggctcccc tcgaacgtgt cgcgtccgac gccgtcgtaa acgacctgca ctccggcgcc
     2581 gtcggtcagc cgtcgcactt ccgcgacgaa gtcctccgtc cggtagtgga tggtctggtc
     2641 ggcgccggcc tcccgcgcca gccgggcctt ctcgtcggtc gagaccgttc cgaagacgcg
     2701 cgcgccgcga agcttcgcca tctgacagag caggaggccg acaccccccg cggccgcgtg
     2761 aacgagacac gtgtctccag gccggagggc gtacgtggac gtcgaaaggt agtgtgcggt
     2821 gatcccctgg agcatgactg ccgcggcttg ccgcgtcgtc acgccgtcgg ggaccggcac
     2881 gacccgcgcg gcggggatcg caacgagc
//
LOCUS       DASWAV010000300         2111 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U2665
            k141_335054, whole genome shotgun sequence.
ACCESSION   DASWAV010000300 DASWAV010000000
VERSION     DASWAV010000300.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960316
            Sequence Read Archive: SRR10260007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2111)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2111)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:47:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,929
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2111
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_335054"
                     /isolate="SMAG_U2665"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.36444 N 132.31889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..454
                     /locus_tag="VF958_06195"
     CDS             <1..454
                     /locus_tag="VF958_06195"
                     /inference="COORDINATES: protein motif:HMM:NF026049.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9148737.1"
                     /translation="ACFDAARRIGEGLVFRPERMARALEEGHATATDLADYLVRKGVA
                     FRTAHEQAGRAVREAEKRGTALAGLPTDVLISICPSADEDFRAVLSPESSVRARRSAG
                     GPAPEAVAGQLELAEAEIADARRWLGGRQPPPIYRAHLRGRLLAEELE"
     gene            451..912
                     /gene="argR"
                     /locus_tag="VF958_06200"
     CDS             451..912
                     /gene="argR"
                     /locus_tag="VF958_06200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006904787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006525 - arginine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HEX9148738.1"
                     /translation="MNRRARERAIVEIIAAHPVGTQDELARELARRGVEVTQATVSRD
                     VKRLGLVKVAGADGAYRYAINGVTGGAHASADDNLRSAFREFVTGLDGGEAFFVVKTL
                     PGRANAVGIAIDEARRSEVAGTIAGDDTILVIVRKARDRDRARREFEKLLA"
     gene            complement(909..1217)
                     /locus_tag="VF958_06205"
     CDS             complement(909..1217)
                     /locus_tag="VF958_06205"
                     /inference="COORDINATES: protein motif:HMM:NF012334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9148739.1"
                     /translation="MNTFTEVLANELRGTGVKVQVLCPGVVRTEFHVIQGMDPGRFPP
                     DLVMTAQDVVQASLAALGSDDVVCIPGLARSERVAEVHEAERRLFEDSTGGTLADRYR
                     "
     gene            complement(1281..>2111)
                     /locus_tag="VF958_06210"
     CDS             complement(1281..>2111)
                     /locus_tag="VF958_06210"
                     /inference="COORDINATES: protein motif:HMM:NF013283.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BamA/TamA family outer membrane protein"
                     /protein_id="HEX9148740.1"
                     /translation="REFSKFDQPIRQSAIGPNMFYDRRDDIIDPHRGYYFTAATKYAF
                     PLLTANARYTKLSAQSAYFRPFASGVFAVAARAGAIFPRGEELGVPVPIAERFFAGGR
                     STNRAFDTDLLGIPGVTVDPDTRATPHASGTGSCAANYPKLLLNHPELAGYDCDAGPR
                     IVGGNGFLGLNAEFRFPIFGNLGGTVFYDAAQVWKKPGDIRVALEGEDGLRQGVGVGL
                     RYMTPIGPVRVEYGWPLDARTVPFNIKLLEENRDVRDTIPAGARKEKGQFFFSIGYPF
                     "
BASE COUNT          345 a          711 c          733 g          322 t
ORIGIN      
        1 cgcttgcttc gatgccgcgc ggcgaatcgg cgaggggctc gtcttccggc ccgagcggat
       61 ggcccgcgct ctcgaagagg ggcacgcgac ggcgactgat ctcgccgact acctcgtacg
      121 caagggcgtc gctttccgca cggcgcacga gcaggcaggg cgagccgtgc gcgaggcgga
      181 gaagcgcggg acggcgctcg ccggcctccc gaccgacgtg ctgatctcga tctgcccgtc
      241 ggcggacgag gacttccggg ccgttctttc gcccgaaagc tcggtgcgcg cgcgacgctc
      301 cgccggcggt cccgcgcccg aggccgtcgc aggtcaactc gaactcgcgg aagcggagat
      361 cgccgacgcg cgccgctggc tcggaggccg gcaaccgccg ccgatctacc gggcgcacct
      421 tcgcggccgc ctgctcgcgg aagagctcga gtgaatcggc gggcgcgcga aagagcgatc
      481 gtcgagatca tcgccgcgca tccggtgggg acgcaggacg agctcgcgcg cgagctcgcc
      541 cggcgcggcg tcgaggtgac ccaggcgacg gtgtcgcgcg acgtcaagcg gctgggtctc
      601 gtcaaggtcg cgggcgcgga cggcgcctac cgatacgcga tcaacggcgt cacgggaggg
      661 gcgcacgcgt ccgccgatga caatctgcgc agcgcgtttc gcgagttcgt cacggggctc
      721 gacggcggcg aggcgttctt cgtcgtgaag acgctgcccg gccgcgcgaa cgcggtgggc
      781 atcgcgatcg acgaagcacg gcggtccgaa gtcgcgggga cgatcgcggg cgacgacaca
      841 atcctcgtca tcgtgcggaa ggcccgcgac cgcgaccgcg cgcgacgcga gttcgaaaag
      901 cttctcgcct agcggtaccg atcggcgagc gtgccgccgg tgctgtcctc gaagagacgg
      961 cgttccgctt cgtggacctc ggccacacgt tcggaacggg cgagccccgg gatacagacc
     1021 acgtcgtcgg atccgagagc ggcgagtgag gcctgcacga cgtcctgcgc cgtcatgacg
     1081 agatccgggg ggaaacggcc cggatccatt ccctggatca cgtgaaactc ggtccgcacc
     1141 accccgggac agagcacctg cactttcaca cccgttcctc gaagctcgtt ggcgagcact
     1201 tccgtaaaag tgttgatgta cgcctttgct ccgcgtagac ggcgcgcttc ggaaggaagg
     1261 cgccctcgag cgacgccgct ctagaatgga tacccgatcg aaaagaagaa ctgtcccttc
     1321 tcctttcgcg cgccggccgg gatggtgtcg cgaacatctc ggttctcctc cagcagcttt
     1381 atgttgaacg ggaccgtgcg tgcgtccaga ggccaaccgt actccacgcg caccgggccg
     1441 atgggcgtca tgtagcgcag cccgaccccg acgccctggc gcagtccgtc ctcgccctcg
     1501 agcgcgacac ggatgtcccc cggcttcttc cacacctgcg cggcgtcgta aaagaccgtc
     1561 ccgcccaggt ttccgaagat cgggaatcgg aactcggcgt tcaatccgag aaagccgttc
     1621 ccgccgacga tccgcggccc ggcgtcgcaa tcgtatcccg cgagctcggg atggttgagc
     1681 aggagtttcg gatagttcgc cgcgcaggag cctgttcccg acgcatgagg cgtcgcgcgg
     1741 gtgtccggat cgacggtcac gcccgggatg ccaaggaggt ccgtgtcaaa cgcccgattg
     1801 gtcgatcggc cgccggcgaa gaagcgctcc gcgatcggaa ccgggacccc gagctcttcg
     1861 ccgcggggga agatcgctcc ggcgcgcgcc gccaccgcaa atactccgct cgcgaacggt
     1921 cggaagtagg cgctctgcgc ggacagcttc gtgtagcgcg cgtttgccgt gagaagcggg
     1981 aacgcgtact tcgtcgccgc cgtgaagtag tagccgcggt gggggtcgat gatgtcgtcg
     2041 cgcctgtcgt agaacatgtt cgggccgatc gcggactgac gaatcggctg gtcgaacttg
     2101 ctgaactccc g
//
LOCUS       DASWAV010000301         2358 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U2665
            k141_176643, whole genome shotgun sequence.
ACCESSION   DASWAV010000301 DASWAV010000000
VERSION     DASWAV010000301.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960316
            Sequence Read Archive: SRR10260007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2358)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2358)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:47:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,929
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2358
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_176643"
                     /isolate="SMAG_U2665"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.36444 N 132.31889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            84..215
                     /locus_tag="VF958_06215"
     CDS             84..215
                     /locus_tag="VF958_06215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002716081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9148741.1"
                     /translation="MIRRLASGGYRLYSRKKDPKTGKRKNLGTFKSLAAAKEHERAV"
     gene            230..826
                     /locus_tag="VF958_06220"
     CDS             230..826
                     /locus_tag="VF958_06220"
                     /inference="COORDINATES: protein motif:HMM:NF024273.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HEX9148742.1"
                     /translation="MSSSGSAGGALSGELAEYHRQVREILADVEKLLEGISDSRLNWR
                     PSPEKWSIAQCLDHLNSGWGALPKLDRAIAGAGERGIRGAGPYRHPLLGKVYLRFTEP
                     PPKIRVSAPRRFRPVAERSISDVVPRFLGLQQEILARIESADGLDLGRIRLSSPISRR
                     FRMSLGQWFAFLAAHERRHLWQAWQVRRHPEFPAGDSR"
     gene            complement(823..1176)
                     /locus_tag="VF958_06225"
     CDS             complement(823..1176)
                     /locus_tag="VF958_06225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011685275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-binding protein"
                     /protein_id="HEX9148743.1"
                     /translation="MTPAPIRPEAPLAALEAVDIRVGTIESVADLPGSKKLLRLVVDF
                     GDHKRTILTGMKGERADPQEIAGRQALFVVNLPPRRMAGEVSEGMLLDLGFADGITPA
                     LAVPERPVPNGTRAG"
     gene            complement(1178..1720)
                     /locus_tag="VF958_06230"
     CDS             complement(1178..1720)
                     /locus_tag="VF958_06230"
                     /inference="COORDINATES: protein motif:HMM:NF014346.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HEX9148744.1"
                     /translation="MRFVCALVLLLTPVCGTAQQPQSPPVAAQPATAPTAVAAPTPSS
                     SASPPAALLPNPSQDLNDQIARAVAEKIAGRENEPAEKVFKNIQSLKGMPAGRFVRVM
                     NMGYSRALGVSCYHCHLGWDYSSDEKRPKRAAREMMDMNRMINEQLRSLQNLEGKPEE
                     HFASCSTCHRGHTDPNTGLH"
     gene            1750..>2358
                     /locus_tag="VF958_06235"
     CDS             1750..>2358
                     /locus_tag="VF958_06235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010961828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016805 - dipeptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HEX9148745.1"
                     /translation="MKQFLIRPGGTMQRLSSAWRLAALSLACAVLLAACSTIPNRPPV
                     EPGPFRQADLVEIVKVDPTIHLDVRYATSNNFTGRPVYQQARAFLQRPAAEALARVSR
                     SLREKGYGLLVFDGYRPWSVTKHFWEITPKDKRENGFVANPAKGSKHNRGAAVDLSMY
                     DLATGKEVVMPSTYDEFSDRADPDYAGGTAEERARRDLLRTKM"
BASE COUNT          389 a          794 c          774 g          401 t
ORIGIN      
        1 gccgccggcg ccggagaaga agccggcgac taagagctag ccggcttcgc cgcgggcgcg
       61 ggtcacacgg atatcctacc ggcatgattc gcaggctcgc atcgggcgga taccggcttt
      121 actcgcgcaa gaaagacccg aagacgggga agcggaagaa cctcgggacg ttcaagtcgc
      181 tcgcggccgc aaaagaacac gagcgggccg tgtagttctt caagcggcag tgagcagctc
      241 cggttcggct ggcggggcgc tttctggcga gctcgccgag taccaccggc aggtccgcga
      301 gattctcgcc gacgtcgaga agctcctcga gggaatctcc gacagccggc tcaactggag
      361 accttcgccc gagaagtggt cgatcgcgca gtgcctggac catctcaact cggggtgggg
      421 cgcgcttccg aagctcgacc gcgcgatcgc cggcgccggc gagcggggaa ttcgcggcgc
      481 cggtccgtac cgccacccgc tcctcggcaa ggtctatctc cggttcacgg aaccgccgcc
      541 aaagatccgc gtctccgctc caagacgctt ccgaccggtc gccgaacgat cgatctccga
      601 cgtggtcccg cgcttcctcg ggctccagca ggagatcctc gcgcggatcg agtccgccga
      661 cgggctcgac ctggggcgaa tccgtctctc gtcaccgatc tcgcgacggt tccgaatgtc
      721 gctcggccag tggttcgcgt ttctcgccgc gcacgagcgg cgtcacctct ggcaggcgtg
      781 gcaggttcgc cggcacccgg agttccccgc gggcgactct cgctagcccg ctcgcgttcc
      841 gtttgggacc ggtcgctcgg ggaccgcgag cgccggagtg atgccgtcgg cgaagccgag
      901 gtcgaggagc atcccctccg aaacctcgcc ggccatccgg cgcggcggca ggttgacgac
      961 aaaaagcgcc tgacgccctg cgatctcctg cggatccgcg cgctcgcctt tcatgcctgt
     1021 gaggatcgtc cgcttgtgat ccccgaagtc gacgacgagc cggagaagtt tcttcgaccc
     1081 gggaagatcc gcgaccgact cgatcgtgcc gacccggatg tcgacggcct cgagcgcggc
     1141 gagcggcgcc tccggtctga tgggcgccgg tgtcatctca gtgcagtcct gtatttggat
     1201 cggtatgccc ccggtggcac gttgagcagc tcgcgaagtg ctcctcgggc ttgccctcca
     1261 ggttctgaag gctccgtagc tgctcgttga tcatgcggtt catgtccatc atctcgcgcg
     1321 ccgcacgctt cggccgcttc tcgtcgctcg aatagtccca accgagatgg cagtgatagc
     1381 aggagacgcc gagcgcgcgg ctgtatccca tgttcatgac gcggacgaat cgcccggcgg
     1441 gcatgccctt caacgactga atgttcttga acaccttctc cgccggctcg ttctcccgcc
     1501 ccgcgatttt ctcagcgacg gcacgtgcga tctggtcgtt caagtcctga ctcgggttcg
     1561 gcaagagagc agcgggtggc gaggcagagg acgatggtgt gggcgctgcc accgcggtcg
     1621 gtgcggttgc gggttgcgcg gcgaccggcg gcgactgcgg ctgttgagcg gtgccgcaaa
     1681 cgggcgtgag caggagaacg agagcgcaaa cgaatctcat cgcatcctcc ttgatatggg
     1741 caacgactga tgaaacagtt tctcatccgt ccggggggaa ccatgcagcg cctctcgtcc
     1801 gcctggcggc ttgccgccct cagtctcgcc tgtgccgtac ttctcgcggc gtgctcgacg
     1861 attccgaacc gtcctcccgt cgagcccggc ccgttccggc aggcggacct cgtcgaaatc
     1921 gtgaaggtcg acccgaccat ccacctggac gtacgctacg ccacctcgaa caacttcacc
     1981 ggccggccgg tctaccaaca ggcgcgggca tttctccagc gacccgcggc cgaggcgctc
     2041 gcgcgcgtca gccggtcgct tcgcgagaaa ggttacggac ttctcgtctt cgacggctac
     2101 cgcccgtggt cggtgacgaa acacttctgg gagatcacgc cgaaggacaa gcgcgagaac
     2161 ggcttcgtcg ccaacccggc gaaggggtcg aagcacaacc ggggcgcggc ggtcgacctc
     2221 tcgatgtacg acctcgcgac cggtaaagag gtggtcatgc cgagcactta cgacgagttc
     2281 tcggatcgcg cggacccgga ttacgcgggc ggcaccgcgg aagagcgcgc gcggcgcgac
     2341 ctcctgcgga ccaagatg
//
LOCUS       DASWAV010000302         2804 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U2665
            k141_255693, whole genome shotgun sequence.
ACCESSION   DASWAV010000302 DASWAV010000000
VERSION     DASWAV010000302.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960316
            Sequence Read Archive: SRR10260007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2804)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2804)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:47:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,929
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2804
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_255693"
                     /isolate="SMAG_U2665"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.36444 N 132.31889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1062)
                     /locus_tag="VF958_06240"
     CDS             complement(<1..1062)
                     /locus_tag="VF958_06240"
                     /inference="COORDINATES: protein motif:HMM:NF025104.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HEX9148746.1"
                     /translation="MLKQKARAIAFGVLVGELALTALALPVAYLIRHGILAPLLPSLF
                     PVAPAFDRYMVLLFLVLPIFGPLLYAVGFHRSHRTLPLAEEIWSVGKVAFGGTALLTL
                     FVYGLRLEFVSRWFLAVFGVVTFFFLAATRVALRLLSRWVRTRGYNFRTVLLVGTGPK
                     AAKLADFIEAHPHWGFNVLGYLDDENGGEIRRSNRWMCLGHIRDLENILVTEVVDEVI
                     FVIEKGKLGEYEEALLATERHGVRSHVSLDIFPHVLARPVLEELDGVPLLTFTTTPSN
                     PMQLVVKRMIDLVLSLLLSLLTLPIQLLAALAIKLTSRGPVFFRQVRCGLNGRTFTLL
                     KFRTMEADAEGRLPEIAHLN"
     gene            complement(1102..2223)
                     /locus_tag="VF958_06245"
     CDS             complement(1102..2223)
                     /locus_tag="VF958_06245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011689066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1972 domain-containing protein"
                     /protein_id="HEX9148747.1"
                     /translation="MKIGILGTRGIPARYGGFETLAEELSARLAARGHDVTVYTRTRY
                     AQPGLREWRGAKVRVLPTIPTKYLDTVVHGALSGFDAAFERFDVVLVCNAINAAACFL
                     PRLAGRTRVVLNVDGLERHRRKWSAVGRLAYRISEKLSTMVPDAVVSDARVIRDYYRE
                     HYGFESHFIPYGGDLPEPTGAETLERLGLARERYMLYVSRLEPENNADAVVRAYRDVP
                     GETPLVVVGDAPYAADYVARVKAMADPRVRFVGAIYGEGYRQLLSSAAVYVQATEVGG
                     THPALVEALGYGRVVCYNSTSENEEVAGGAAVPFDVRKPETLTRLLVQILDDPAAWSV
                     WKERARKRTQERYRWDDVADRYEKVLRGDAELEGAARIP"
     gene            complement(2220..>2804)
                     /locus_tag="VF958_06250"
     CDS             complement(2220..>2804)
                     /locus_tag="VF958_06250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEX9148748.1"
                     /translation="IVFTASGRHFDRGSDEEGEGRYLEEEEVAGTTAAAGLYRREALE
                     SAKISTGYFDSDFFLYREDADLAWRLANLGWKCLYVPTAVAWHRRTNLPERRRVQSVE
                     ANRHSVKNRFLLRINNQSTADFWRTLVPTFARDLVVLAACLTVERSSLPAFGWLWRNR
                     RRLLEKRREIRELVRKREPLSPRERGRGEGVASP"
BASE COUNT          491 a          932 c          957 g          424 t
ORIGIN      
        1 gttcaagtga gcgatctcgg gcagccgccc ctccgcgtcc gcctccatcg tgcggaactt
       61 cagcagcgtg aaggtgcggc cgttcagacc gcagcgcacc tggcggaaga acaccggccc
      121 gcgcgacgtc agcttgatcg cgagagccgc caggagctgg atgggcagcg tcagaagcga
      181 gagcaggagc gaaaggacga ggtcgatcat ccgcttcacg acgagctgca tcgggttcga
      241 cggagtcgtc gtgaacgtca ggagcggcac gccgtcgagc tcctcgagaa cgggccgcgc
      301 gagcacgtgc gggaatatgt cgagcgagac gtgcgatcgc acgccgtgcc gctcggtagc
      361 gaggagggcc tcctcgtact cgccgagttt gcccttctcg atgacgaaga tcacttcgtc
      421 gacgacttcc gtcacgagga tgttctccag gtcccggatg tgtccgaggc acatccaccg
      481 gttggaccgg cggatctcac cgccgttctc gtcgtcgagg tatccgagga cgttgaatcc
      541 ccagtgcgga tgcgcctcga tgaagtcggc gagcttcgcc gccttcgggc cggtccccac
      601 gaggagcacc gtgcggaagt tgtagccgcg cgtacgcacc cagcgcgaca ggagccggag
      661 cgcgacgcgc gtcgccgcga ggaagaagaa cgtcacgact ccgaagaccg cgaggaacca
      721 ccggctcacg aactcgaggc gcagaccgta gacgaagagc gtcaggagcg ccgtgccgcc
      781 gaacgcgact ttgccgaccg accagatctc ttcggcgagc ggcagcgtgc ggtggctccg
      841 gtggaagccg accgcgtaga gcagcgggcc gaaaatcggc aggaccagga agagcaacac
      901 catgtaccgg tcgaacgcgg gcgcgaccgg gaagagcgag ggaaggaggg gcgcgagaat
      961 tccgtggcgg atcaggtacg cgacgggaag ggcgagggcg gtcagggcga gctcccccac
     1021 gagtaccccg aaagcgatcg cacgagcttt ctgcttcagc atgaatgaaa ccggcggcgg
     1081 ggccgcgggg cggcggcgat gttacgggat gcgggccgct ccttcaagct ctgcgtcacc
     1141 gcgcaaaacc ttctcgtatc gatcggccac gtcgtcccag cggtagcgct cctgcgtccg
     1201 ttttcttgcc cgttccttcc atacggacca ggcggcggga tcatccagaa tctgcaccag
     1261 gagccgcgtg agcgtttcgg gcttgcggac gtcgaaaggg accgcggcgc cccccgcgac
     1321 ctcctcgttc tccgaagtcg agttgtagca aacgacgcgc ccgtacccga gcgcctcgac
     1381 gagcgcaggg tgagtcccgc ccacttcggt cgcctgcacg tagaccgccg cggacgagag
     1441 gagctgtcga taaccctcgc cgtagattgc gccgacgaac cggacgcgcg ggtcggccat
     1501 cgctttgacg cgggcgacgt aatcggcggc atacggcgca tcgccgacga cgacgagagg
     1561 cgtctcgccg ggaacgtcgc gatacgcgcg gaccaccgcg tcggcgttgt tctccggctc
     1621 gaggcgcgac acgtacagca tgtaccgttc gcgcgcgagg ccgaggcgct cgagcgtctc
     1681 cgcgccggtc ggctccggca ggtcgccgcc gtacgggatg aagtgggact cgaaaccgta
     1741 gtgctcgcgg taatagtcgc ggatcacgcg ggcgtcggac acgaccgcgt ccggcaccat
     1801 cgtcgagagc ttctccgaga tccggtacgc gagtcttccc accgcgctcc acttccggcg
     1861 atggcgctcg agcccgtcga cgttcaggac gacccgcgtg cgcccggcga gacgcggcag
     1921 gaaacaggcc gccgcgttga tcgcgttgca gacgagcacg acgtcgaacc tttcgaacgc
     1981 ggcgtcgaac ccggagaggg ctccgtgaac gaccgtgtcg aggtacttcg tcggaatcgt
     2041 cgggagcacg cggactttcg cgccgcgcca ctcgcgcagt cccggctgcg cgtagcgggt
     2101 gcgcgtgtac accgtgacgt cgtgtccgcg cgccgccagg cgggcggaga gctcctcggc
     2161 gagagtctcg aagccgccgt aacgcgcggg aatgccgcgg gtgccgagga tgccgatttt
     2221 cacggcgagg caaccccctc acccctgccc ctctcccggg gggagagggg ttctcttttc
     2281 cgcaccagct cccggatttc gcgacgtttc tccaacagac gccgtcgatt tcgccacaac
     2341 cacccaaacg ccgggagcga cgagcgctcg accgtcaggc acgcggccag cacgacgagg
     2401 tcgcgcgcga acgtcggaac gagcgttcgc cagaagtccg cggtcgactg gttgttgatg
     2461 cgcagcagga accggttctt gaccgagtgt ctgtttgcct ccaccgactg gacgcggcgg
     2521 cgctcgggca gatttgtccg ccggtgccac gcgaccgccg tcggcacgta gaggcacttc
     2581 catcccaggt tcgcgagccg ccacgcgaga tcggcgtctt cgcggtagag gaagaagtcc
     2641 gagtcgaagt acccggtcga gatcttcgcc gactcgagcg cttcgcggcg gtaaagcccg
     2701 gcggcggccg tcgttcccgc cacctcctct tcttcgaggt agcgcccctc tccctcttcg
     2761 tcgcttccgc ggtcgaagtg gcggcccgaa gcggtgaaga cgat
//
LOCUS       DASWAV010000303         2577 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U2665
            k141_96382, whole genome shotgun sequence.
ACCESSION   DASWAV010000303 DASWAV010000000
VERSION     DASWAV010000303.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960316
            Sequence Read Archive: SRR10260007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2577)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2577)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:47:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,929
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2577
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_96382"
                     /isolate="SMAG_U2665"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.36444 N 132.31889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..910)
                     /locus_tag="VF958_06255"
     CDS             complement(<1..910)
                     /locus_tag="VF958_06255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9148749.1"
                     /translation="MTGLAVPFARVAAVLGVATVLGWGTATALLPRRGTRAERLAWGF
                     AIGLAIIALSVPLGFALRSRPGWLPFLLLSVLVVLPARILRLRDQEPERRLFPIDALT
                     VFLALLLVAGVALYALRALTEPMWANDYLAIWGLKGKTFFAEGRVPERLFQWTSLRFS
                     HPEYPLGLPFLYAGIAYLSGGWDDHAMALLFPLLQLATLGALFGWLRRRGADRPLALG
                     TAALLSHFEPLYRGFTTGMADVPLSFGVLLLGTSLCDRMERADRGASRRLALASLACV
                     GTKNEGLFFVAAGVVVAALFAFSRRGV"
     gene            complement(907..1329)
                     /locus_tag="VF958_06260"
     CDS             complement(907..1329)
                     /locus_tag="VF958_06260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9148750.1"
                     /translation="MTGRRLAVLSAGAALLLLFLLADSPRELARRARFLARSASSELA
                     LRRLSGSGAAFDRKFFVFLVALRRSLPSDARGVALYVPHPSTQELYLASYQLAPVPVL
                     LAPARVPPRWLAALYGPPPPGEWRVIAPLPGGTLAAPK"
     gene            complement(1326..2543)
                     /gene="glyA"
                     /locus_tag="VF958_06265"
     CDS             complement(1326..2543)
                     /gene="glyA"
                     /locus_tag="VF958_06265"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012818227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004372 - glycine hydroxymethyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0070905 - serine binding [Evidence IEA];
                     GO_process: GO:0019264 - glycine biosynthetic process from
                     serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HEX9148751.1"
                     /translation="MDPEVARWIHDEELRQNGGLELIASENFVSRAVREAQGSVLTNK
                     YAEGYPGRRYYGGCEFVDKIEALAIERVKKLFGAEHANVQPHAGSQANMAVYMTVLKP
                     GDTILGMDLSHGGHLTHGHPLSYSGFEYKVVAYGVRREDERIDYDQLAALAREHRPGL
                     IIAGASAYPREIDFVRFAEAAAESGSVLMVDIAHIAGLIIAGLHGSPVPHAPFVTSTT
                     HKTLRGPRGGFILCRADRAKDLDRKVFPGVQGGPLEHVIAAKAVAFAEAGTPEFVEYQ
                     RRVVENAKTLAARLTARGLRLVSGGTDNHLMLVDVGAAGLSGKEAEKMLEAVGLTVNK
                     NTIPFDTRPPLVASGIRIGTPAVTTRGMGTAEMEAIGDAIGRVLESRGEAAVLQEVRS
                     AVSELCAAYPLPV"
BASE COUNT          446 a          872 c          937 g          322 t
ORIGIN      
        1 cgactcctcg ccgggaaaac gcgaagagtg ccgcgacgac gacgcccgcg gcgacgaaga
       61 agaggccttc gttcttcgtg ccgacgcagg cgagcgaagc gagagcgagc cggcgcgacg
      121 cgccgcggtc cgcgcgctcc atgcgatcgc agagcgaggt gccgagcagc agcacgccga
      181 aggagagcgg gacgtcggcc attccggtgg tgaatccgcg gtagagcggc tcgaagtgcg
      241 agagcagcgc agcggtcccc agggcgagcg gccggtccgc tccgcggcgc ctcagccaac
      301 cgaacaatgc cccgagtgtg gccagctgca gcagcggaaa gaggagcgcc atcgcgtggt
      361 cgtcccatcc cccggacagg tacgcgatcc cggcatagag gaacggaagc ccgagcgggt
      421 actccggatg cgagaaccgg agagaggtcc actggaagag gcgctcgggc acgcgccctt
      481 cggcgaagaa ggtcttgccc ttcaaacccc agatcgcgag gtagtcgttc gcccacatgg
      541 gctccgtcag cgcccggagc gcgtagagcg cgacgccggc aacgagaagg agagcgagaa
      601 agaccgtcaa cgcgtcgatc ggaaacaagc gccgctccgg ttcctggtcg cggagccgga
      661 gaatgcgggc cggcaggacg acgagcaccg agaggaggag aaacggcagc cagccgggcc
      721 ggctgcggag ggcgaacccc aggggaaccg aaagagcgat gatcgcgaga ccgatcgcga
      781 atccccaggc gagccgctcg gcccgggttc ctctccgggg caggagcgcg gtcgccgtcc
      841 cccagcccag aaccgtagcg acgccgagca ccgccgcgac ccgtgcgaac ggtaccgcga
      901 gcccggtcac ttcggggccg ccagcgtgcc gcccggcagc ggtgcaatga cccgccactc
      961 ccccggcggc ggcggcccgt acaacgcggc gagccaccgc ggcgggacgc gtgcgggtgc
     1021 gagcagcacg ggcaccgggg cgagctggta ggaggcgaga tacagctcct gagtcgaggg
     1081 gtgcggcacg tacagcgcca cgccgcgcgc gtcgctcggc aagcttcggc gaagcgccac
     1141 gaggaagacg aagaactttc gatcgaaggc agcgcccgat ccggaaagcc ggcggagcgc
     1201 gagctcggac gaggccgagc gtgcgaggaa gcgggcgcga cgggccagct cgcgcggcga
     1261 gtcggcgagc aggaacagga gcagcagcgc ggcgcccgcg gagaggaccg cgagcctccg
     1321 ccccgtcaca ccggaagggg gtatgcggcg cagagctccg agacggcgga gcggacctct
     1381 tgcagcacgg cagcttcgcc gcgcgactcg aggacgcggc caattgcgtc gccgatcgcc
     1441 tccatttccg ccgtccccat cccgcgggtc gtcacggcgg gcgtcccgat ccggattccg
     1501 gacgcgacga ggggcggacg cgtgtcgaac ggaatcgtgt ttttgttgac ggtcaatccg
     1561 acggcttcga gcatcttctc ggcctccttg cctgagaggc cggccgcgcc cacgtccacg
     1621 agcatgaggt ggttgtcggt gccgccggag acgagtcgga gaccgcgggc cgtgaggcgc
     1681 gccgcgagtg tcttcgcgtt ctcgacgacc cggcgctgat attcaacgaa ctcgggggtc
     1741 cccgcctcgg cgaacgcgac ggccttggcc gcgatcacgt gctcgagcgg cccgccctgc
     1801 acgccgggga acaccttccg atcgaggtcc ttcgcgcggt ccgcccggca gaggatgaag
     1861 cctccgcgcg gcccgcggag ggtcttgtgc gtcgtcgacg tcacgaacgg ggcgtgcgga
     1921 acgggcgacc cgtgcagccc cgcaatgatg agcccggcaa tgtgcgcgat gtccaccatg
     1981 aggacggaac cgctctccgc tgcggcttcc gcgaatcgca caaagtcgat ctcccgggga
     2041 tacgcggacg cgcccgcgat gatgaggccc ggccggtgct ctcgcgcgag cgcggcgagc
     2101 tggtcgtagt cgatgcgctc gtcctcgcgg cggacgccat acgccacgac tttgtattcg
     2161 aagccggagt aggagagcgg gtgcccgtgg gtcaggtgac ctccgtgcga aaggtccatc
     2221 ccgaggatcg tgtcgccggg cttcagcacg gtcatgtaga cggccatgtt cgcctgcgac
     2281 ccggcgtgcg gctggacgtt tgcatgctcg gcgccgaaga gcttcttcac gcgctcgatc
     2341 gcgagggcct cgatcttgtc gacgaactcg cagccgccgt agtaccggcg acccggatat
     2401 ccctccgcgt acttgttcgt gaggaccgag ccctgcgcct cgcgcaccgc gcgcgacacg
     2461 aagttctccg atgcgatgag ctcgaggccg ccgttctgcc ggagctcctc atcgtggatc
     2521 cagcgggcga cctccgggtc cacgaccgcg aggtcgcggt cgaggaacgc cgccgcg
//
LOCUS       DASWAV010000304         2967 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U2665
            k141_573963, whole genome shotgun sequence.
ACCESSION   DASWAV010000304 DASWAV010000000
VERSION     DASWAV010000304.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960316
            Sequence Read Archive: SRR10260007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2967)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2967)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:47:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,929
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2967
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_573963"
                     /isolate="SMAG_U2665"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.36444 N 132.31889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..855
                     /locus_tag="VF958_06270"
     CDS             <1..855
                     /locus_tag="VF958_06270"
                     /inference="COORDINATES: protein motif:HMM:NF039482.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+)/NADH kinase"
                     /protein_id="HEX9148752.1"
                     /translation="FRRVGLACKCTSRAALALTRSLDSALESRGLDVRFDTDTGAALG
                     RSGGLPRERLARSVDLVIVVGGDGTLLSVARAAPTSTPVLGINVGVLGFLAGLSRGEA
                     LTRLDEVLTGKFREDRRRRIEVTVTAMRRTRRFRALNDAVLNKEALARISTFRIDLDG
                     RPVSEFRADGVIVSTPTGSTAYNLSAGGPILHPGLPALVITPICPHTLSQRPLVVPSS
                     TRIGLAVIDPGRRPGGVYLTIDGQEGLPIGPDCPVEVHSSASPVTLLRPPEADHFRNL
                     AEKLNWGI"
     gene            874..>2967
                     /locus_tag="VF958_06275"
     CDS             874..>2967
                     /locus_tag="VF958_06275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9148753.1"
                     /translation="MSEEPLSDEHPHRALRHLSALRLFGISILFLVLAGYAVWTSERF
                     QTLFQGVSESRISQALGRPITFRKVEVRFLPPSLRLADVRIGNDPRAGAGPFLSADEL
                     SIGGGVSLSGNELRLGRIRAVRPKIALVQFPDGSWNLPPGISRPARKGGLKVRVGEVV
                     LQSGTFELEGRKMELSVALDDFAGELTAIGEDHYTGVLGSRRMILRLPDAEPIVSDLT
                     TRFHMEPGRGIVFETLRVKGDFGTLAAAGTIETGGLQKTTLVAGGDVSITEIERIFHS
                     RLGFLGNGRVDARIEIPSGGDFRITGRVTAPRVDARGFLLEDLEASVDARRDELVAKI
                     EQGRYAGGSANGVVRIASLVSKPRLFTIALEGQGLSLERFFADLDLPGIGLSGSADVT
                     VAIRFPEEIGIEHANGGGALEVHPGPPASLVRGRHGIPTGGGGPLSIANGRIGFEGVT
                     LRFPQTTVDLTGGLRIGEWKPDFDFTLKSRDLTEVDLFYQNIVAATGGAPEPLGVGGT
                     GEASGHLAGQWGDPDATVQFSAEETRWGGVNFGSVRGTADVHEGAFLFRPLRAYDGDA
                     SLSLEGTVRFRELRGRPKLDLEVAAHEYPLARLLEYLDFDYPVSGFVTGSFPIAGSTT
                     SLTGGGPVSLRDASVWGQKIPLVTGSVRFSPGRFELADVRAELGGGMVGGSGAIQIQE
                     KTFEARFAGDAIPLES"
BASE COUNT          428 a         1032 c         1012 g          495 t
ORIGIN      
        1 tttcggcgtg ttggtctcgc ctgcaagtgc acgtcccggg cggcgctcgc cctgacgcgg
       61 tcgctcgact cggccctcga gagccgcggc ctcgacgtcc ggttcgacac cgatacaggt
      121 gccgccctcg gccggtccgg cggtctccct cgcgagcgcc tcgcccgctc cgtcgacctg
      181 gtgatcgtcg tcggcggaga cggcacgctc ctctccgtcg cacgcgccgc gcccacctcc
      241 acgccggtgc tcgggatcaa cgtcggcgtc ctcgggttcc tggcgggcct gtcgcgcggc
      301 gaggcgctca cgcgtctgga cgaggtgctg accgggaaat tccgtgagga ccggcggcgc
      361 cggatcgagg tcaccgtcac ggcaatgcgg cggacgaggc gcttccgcgc gctgaacgac
      421 gccgtcctga acaaggaggc gctcgctcga atctcgacct tccgcatcga cctggacggt
      481 cgcccggtgt ccgagttccg cgccgacgga gtcatcgtct cgacgccgac cggctcgacc
      541 gcgtacaacc tttctgccgg gggtccgatc ctccatccgg ggcttccggc gctcgtcatt
      601 acaccgatct gtccccacac gctctcgcag cgtcccctcg tcgtgccgtc ctccacgcgg
      661 ataggactcg ccgtcatcga cccgggccgc cggccgggcg gcgtctacct gacgatcgac
      721 ggacaggagg ggctccccat cggcccggac tgtccggtcg aagtgcactc gagcgcctcc
      781 cccgtcacgc tcctgcgtcc ccccgaagcc gaccacttcc ggaacctggc ggagaaattg
      841 aactggggaa tctgacgtga ccgaacttca atcttgagcg aagaacctct ttccgacgag
      901 catccgcatc gcgcgctccg gcacctttcg gcgctgcgac tgttcggcat atcgatcctg
      961 tttctcgtgc tcgcggggta cgccgtgtgg acctccgaga ggtttcaaac gctcttccag
     1021 ggcgtctccg agtcgcgcat ctcccaggct ctcggccgtc cgatcacgtt tcgcaaggtc
     1081 gaggtcaggt ttctgccgcc atcgctccgg ctcgccgacg tccggatcgg caacgatccg
     1141 cgggcgggcg ccggaccgtt tctctcggcg gacgagctct ccatcggcgg cggcgtgtcg
     1201 ctttcgggca acgagctccg cctcgggcgg atccgcgcgg tgcgcccgaa gatcgctctc
     1261 gtgcagttcc ccgacgggtc ctggaatctc ccccccggga tcagccggcc cgcgcgaaag
     1321 ggcggactca aagtgcgcgt cggcgaagtc gtcctccaaa gcgggacgtt cgagctggaa
     1381 ggtcggaaga tggagctctc cgtcgcgctc gacgacttcg cgggggagtt gacggcgatc
     1441 ggcgaggacc actacacggg ggtgctcggg tcgcggcgga tgatcctccg acttccggac
     1501 gcggagccga tcgtctcgga cctgacgacc cggtttcaca tggagcccgg acgcggaatc
     1561 gttttcgaga cgctgcgcgt caagggggac ttcgggacgc tcgccgccgc cgggacgatc
     1621 gagaccggcg ggttgcagaa gacgacgctc gtcgctggcg gcgacgtgtc gatcaccgaa
     1681 atcgaacgga tctttcactc gcgcctcggc ttcctgggga acgggcgcgt cgacgcccgc
     1741 atcgagatcc cctccggcgg agacttccgc atcaccggac gggtgacggc gccgcgcgtc
     1801 gatgcgcgcg gattcctgct cgaagacctg gaagcgtcgg tggatgcgcg gcgcgacgag
     1861 ctcgtcgcga agatcgaaca agggcgctac gcgggcggct cggcgaacgg cgtcgtccgc
     1921 atcgcgagcc tcgtctcgaa accgcggctc ttcaccatag cgctcgaggg gcagggtctg
     1981 tcgctcgagc gcttcttcgc ggacctcgac cttcccggca tcggcctctc cggctcggcg
     2041 gatgtcacgg tcgcgatccg ctttcccgag gagatcggca tcgagcacgc gaacggcggc
     2101 ggtgccctcg aagtccatcc cgggccgccg gcgtcgctcg tgcgcggaag acacggcatt
     2161 ccgacgggag gcgggggtcc gctttcgatc gccaacggcc gcatcgggtt cgaaggcgtc
     2221 acgctccggt tcccgcagac aacggtcgat ctcacgggcg gtctccgcat cggagagtgg
     2281 aaaccggact tcgacttcac gctgaagtcg cgcgacctga ccgaggtgga cctcttctac
     2341 cagaacatcg tcgcagcgac cgggggtgcg ccggagcccc tgggagtcgg cggtacggga
     2401 gaggcgagcg gccatctcgc cgggcaatgg ggcgatcccg acgcgacggt ccaattttcc
     2461 gcggaggaga ctcgctgggg gggcgtgaac ttcggaagcg ttcgcggcac cgccgacgtc
     2521 cacgagggcg ccttcctctt ccggccgctg cgcgcctacg acggggatgc gagcctgtcg
     2581 ctcgagggca cggtgaggtt ccgcgaactg cgcggaaggc cgaagctcga cctggaggtg
     2641 gccgcccacg agtatccgct ggcaagactg ctcgagtatc tcgacttcga ctacccggtc
     2701 agcggtttcg tcaccggaag ctttcccatc gccggttcga ccacctcgct caccggcggg
     2761 ggcccggttt ccctgcggga tgcctccgtg tggggccaga aaatcccgct cgtcaccgga
     2821 agcgtgcggt tctcgccggg ccggttcgag ctggcggacg tccgcgcgga gctcggcggc
     2881 ggaatggtcg gcgggagcgg cgcgatccag atccaggaga agacgttcga ggcgcgcttc
     2941 gcgggcgatg cgattccgct cgagtcg
//
LOCUS       DASWAV010000305         6937 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U2665
            k141_55169, whole genome shotgun sequence.
ACCESSION   DASWAV010000305 DASWAV010000000
VERSION     DASWAV010000305.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960316
            Sequence Read Archive: SRR10260007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 6937)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6937)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:47:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,929
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6937
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_55169"
                     /isolate="SMAG_U2665"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.36444 N 132.31889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..533
                     /locus_tag="VF958_06280"
     CDS             <1..533
                     /locus_tag="VF958_06280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020477670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1259 domain-containing protein"
                     /protein_id="HEX9148754.1"
                     /translation="GETPRVMFLHVGGTGEEAALAATIGKVFATIREMSPARTEFREP
                     SINPASTSLDSAKLDALLGQKGELKDGVYKIVVGRTVRVHGADAGAAMGVNTWAAFAG
                     SEGRAVVDGDFATLASDLQPVLKALTGAGIDVVAIHNHMTGEEPRIVFLHYWGIGRAE
                     DLARGVRGALDRVKGS"
     gene            complement(530..1237)
                     /locus_tag="VF958_06285"
     CDS             complement(530..1237)
                     /locus_tag="VF958_06285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9148755.1"
                     /translation="MSLRRFAALAAGILLWLSPAAVRAHKDDYLADTFVFLTLEAREL
                     ELEYWLDAQDGPRGLLHTWGAEYGITDHLMADLSARWLEREGGPTTFRQGFFELRYRF
                     GEEGRHVLDPAASVEYEIRRSPDGARRKLLEPRIVLSRDFAGWNATLNLFYAIVVDET
                     RRSAFEGAAGLRSPAFGRWSGGLEIRREVALENETLVIPQVWFRISPEAYVKAGGGKN
                     FAREKNAFVRIAFEIEL"
     gene            1397..2086
                     /locus_tag="VF958_06290"
     CDS             1397..2086
                     /locus_tag="VF958_06290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9148756.1"
                     /translation="MKRILGIAVSVCFLAGLAGLAGAQTAFAQTNVHTETKTKQTGPG
                     PNVKTKTEVTTGTVKEYEAGKKIKVSGPKNKTYSFDLDENARVDGNITVGQRVRVEWM
                     KDNAGKEHVVVLSGPGSTRGAAETAMHEPSAAGHNVHMKSETTVHQPGPNVKTKTEMV
                     IGTVREYEAGKKIKVTGPDEKDFSFDLDEGVGLKGNVAVGERVKVEYTKGDNGNHVTV
                     VTLVPKKKKAA"
     gene            2107..2694
                     /locus_tag="VF958_06295"
     CDS             2107..2694
                     /locus_tag="VF958_06295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9148757.1"
                     /translation="MRNRSRIGVAGLLFFWAAWMLFTGTSSATLPIQKKAKALGMPAD
                     NCLYCHNEKLPKKGAVTHNERGKWLIAQKQTRKAKEIDVAWLKDYPGEQAAATASSTG
                     GTMHMKSESTVHKPGPDVKTKTEAVVGTVKEYEAGKKIKVTGPAGKDVSFDLDKAVTV
                     NGAIAVGQRVTVEYTKSNDGKEHVTVLSLAPPVKK"
     gene            complement(2716..3828)
                     /locus_tag="VF958_06300"
     CDS             complement(2716..3828)
                     /locus_tag="VF958_06300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020717329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEX9148758.1"
                     /translation="MSRFGAEVGGINLAQGLPDFEPPPAVVAALARAIAKPGAHQYSF
                     TWGSPEFRAAVAEKYARFNSMRPDPETEVTITCGVSEAQVAAVLALTEPGDEALILEP
                     WYENYLPACVLAGVRPRFVPLAEPDYALDLDALEKAIRPRTRVLLLNTPGNPSGRVLT
                     REELASVGRLCERAGVIAIVDEIYEHIWYDGRQHVSLGSLPGMEDRTVTLSGLGKSYA
                     VTGWRVGWAVAAPALTSRIRKVHDYLTICAPAPFQEAGRAALRLPDSYYERLRRAYGA
                     RRKILLDAIEQAGFGFTPPQGSYYVMADAAPLGWRDDRSFVEFLARTAGVIAVPGSSF
                     YARGGGRTRVRFNFAKKEVTLRAAARRLTKSDLSAR"
     gene            3932..5323
                     /locus_tag="VF958_06305"
     CDS             3932..5323
                     /locus_tag="VF958_06305"
                     /inference="COORDINATES: protein motif:HMM:NF024910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HEX9148759.1"
                     /translation="MKTTSSGLHRRLTLVEYFTFGFGTMVGVGWVVLMDDWLGRGGPA
                     GASLGFLAGGLLLLPIARTYGRLVARFPDAGGEIAYAESVFPPPAAFGAAWTMVLSYA
                     IVCPWEAVAVGNLLARVFPALNTLPLYEVAGREIYAPRLALGLLVTVLIAAINDRGVR
                     LSGVFQDAATFGLLALFVLFAALAFARGSPGNLPPLFARPGVAGAWLSVLIVLQVVPY
                     FMTGFESVGKGSEEAHPDFPPARFGRAITLAAVCGFLFYATIILAVAWIFPWRELVTG
                     RLGTEAAFARAFGSPLLARLVVLAAFLSLVKIFNGNFLAATRMLYGIARRGLVHPSLA
                     RVNTRNGTPRRAILLVAGFSAVGAFLGDALLVPISEVGSLAVAVGWFTSCVACVALAR
                     RGDDPWRLPGRRRAAIRTAAIGALVAAAIIAMKVLPVVPGSFTRWEWVAFLAWSSLGF
                     GFWLFRRPARPML"
     gene            5393..6331
                     /locus_tag="VF958_06310"
     CDS             5393..6331
                     /locus_tag="VF958_06310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9148760.1"
                     /translation="MPHRARAPFGCLLAAAFAIVPSAAFSEDLERQIDRIQFNLLANA
                     ALVSRATAGDIAVPQWLTVSFAGNATPIEVVTPIAHGLTTGDAVLIKGVLGNVAANGW
                     WTITRTAETRFTLDGSAGNGEYGGGGAVFPAAGQPQPAGARGAGGELPWTPWFSAPAV
                     ARETEFFQPLGELSTFPSVPPVNSFLSQEIDGSLFEPGEALCLSIEARMPESAIGDQR
                     LKMVVTAALGPTRVYSTTFPATLLTPVYQRFALCFRLANDAVPRGGVLRVEFIDEHLQ
                     GIPKPMFWTRPMLNEGVAPAPWTASVEPRTRTRAFR"
     gene            6413..>6937
                     /locus_tag="VF958_06315"
     CDS             6413..>6937
                     /locus_tag="VF958_06315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008643694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HEX9148761.1"
                     /translation="MRDLAQGCRSLVVVGILASIASLFCTTSVRGQGALPIERIKLPP
                     GFEIRVWARAPGARSMTLSPGGTLFVGTRDGNVYAIHAASGGEPSVVTIARGLNMPNG
                     VAFRDGALYVAEIHRVIRYDGIESRLSNPPAPAVVREFPTDRHHGWKFIAFGPDGLLY
                     VPVGAPCNICQPAPM"
BASE COUNT         1133 a         2281 c         2380 g         1143 t
ORIGIN      
        1 tcggcgagac tccgcgggtg atgttcctcc acgtgggagg cacgggagag gaagccgccc
       61 tcgcggcgac gatcggaaaa gtcttcgcga cgatccgcga gatgtcaccg gcccggacgg
      121 agttccggga gccgtcgatc aatccggcga gcacttcgct cgattccgca aagctcgatg
      181 ccctgctcgg gcagaagggc gagctcaaag acggggtcta caagatcgtc gtcgggcgca
      241 cggtccgggt ccacggcgcg gatgcgggcg cggcgatggg agtcaacacc tgggcggcgt
      301 tcgcggggtc ggaagggcgc gccgtcgtcg acggggactt cgcgacgctc gcgtcggatc
      361 tgcagcccgt cctgaaggcg ttgaccggag ccggaatcga cgtcgtcgcc atccacaacc
      421 acatgaccgg cgaggaaccc cggatcgtgt ttctccacta ctgggggatc ggccgcgccg
      481 aggacctggc gcgcggcgtc cgcggggcac tggaccgcgt gaagggaagc tagagctcga
      541 tttcaaacgc gattcgcacg aacgcgttct tctctcgggc gaagttcttt ccgccgcccg
      601 ccttgacgta ggcctcgggc gagatccgga accagacctg aggtatcacc agggtctcgt
      661 tctcgagagc gacctcacgg cggatctcca ggccaccgct ccagcgaccg aatgcgggcg
      721 aacggaggcc cgcggcgcct tcgaaggccg agcgccgggt ttcgtcgacg acgatcgcgt
      781 agaagaggtt caaggtcgcg ttccagccgg cgaagtctcg cgacaggacg atccgcggct
      841 cgagaagctt gcgtcgggcg ccatccggcg atctgcggat ctcgtactcg accgatgctg
      901 ccgggtcgag cacgtgccgc ccttcctcgc cgaaccggta gcgcagttcg aagaagccct
      961 gtcggaaggt ggtcggtccg ccctcccgct cgagccagcg ggccgacagg tctgccatta
     1021 ggtgatcggt gatcccatac tccgctcccc aggtgtgaag gagaccccgg gggccgtctt
     1081 gagcgtcgag ccagtactcg agctcgagct cccgcgcctc gagagtcaga aaaacgaagg
     1141 tgtcggcgag atagtcgtcc ttgtgagcac gaacggcggc tggcgagagc cagagaagaa
     1201 tcccggcggc cagagcagcg aatcggcgaa ggctcatgag cggatcttaa cgacgaccta
     1261 gcggaagcct cgaaaaaccg ctgcggggcc gatccggcag ttctcgcaat tcacccgtcg
     1321 ttcatctcct tggctgtcat gaattttgct tcgttctctc cccggcccat ttggagccag
     1381 tgagagagga ggagtcatga aacgcattct tgggatagcg gtttccgtct gcttcttggc
     1441 cggcttggcc ggcttggccg gcgcgcagac ggcatttgcg cagaccaatg tccacacgga
     1501 gacgaaaacg aagcagaccg gccccgggcc caacgtcaag acgaagaccg aggtcacgac
     1561 cggaaccgtc aaggaatacg aggcgggcaa gaagatcaag gtctcggggc ccaagaacaa
     1621 gacgtactcg ttcgatctcg acgagaacgc ccgcgtcgac gggaacatca ctgtcgggca
     1681 gagggtgagg gtcgagtgga tgaaggacaa cgccgggaaa gagcacgtgg tggtcctgtc
     1741 cggtccgggc tccacgagag gcgcggcgga gacggcgatg cacgaaccgt ccgcggcggg
     1801 ccacaacgtc cacatgaagt cggagacgac cgtccaccag ccggggccga acgtcaagac
     1861 gaaaaccgag atggtcatcg gcaccgtgag ggaatacgag gccggaaaga agatcaaggt
     1921 gacgggaccc gacgagaagg acttctcgtt cgacctcgac gaaggtgtcg gactcaaggg
     1981 gaacgtcgct gtcggcgagc gcgtgaaggt ggagtacacg aagggggaca atggcaatca
     2041 cgtgaccgtg gtgaccctcg tcccgaagaa gaagaaggcg gcttgatcga atgagaggag
     2101 agaaccatgc gcaaccgttc gcggatcggt gttgcgggtc tgctgttctt ctgggcggcc
     2161 tggatgctct tcacggggac ttcgtcggca acgctgccga ttcagaagaa ggcgaaggcg
     2221 ctcggcatgc ctgccgacaa ttgcctctat tgccacaacg agaagcttcc gaagaaaggg
     2281 gcggtgactc acaacgagcg cggcaagtgg ctcattgccc agaagcaaac gcggaaagcc
     2341 aaggagatcg acgtcgcgtg gctgaaggac tacccgggcg agcaggccgc ggcgacggca
     2401 tcgagcacgg gcggcacgat gcacatgaaa tcggagtcga cggttcacaa gcccggaccc
     2461 gacgtgaaga ccaagaccga agcggtcgtg ggtacggtca aggaatacga ggccggtaag
     2521 aagatcaagg tgacgggccc cgccgggaaa gacgtctcgt tcgacctcga caaagccgtg
     2581 acggtcaacg gcgcgatcgc cgtgggtcag cgggtgaccg tcgagtacac gaagtcgaac
     2641 gacgggaagg agcacgtcac cgtcttgtcg ctcgccccgc ccgtcaagaa gtgagcggga
     2701 gggcggctcg gaggctcagc gcgccgagag gtcgctcttc gtcagtcgcc gggcggcggc
     2761 gcgcagagtg acctccttct tggcgaagtt gaagcgcacc cgggtccgcc cgccgccgcg
     2821 tgcatagaag ctcgacccgg gcacggcgat cacgcccgcg gttcgggcga ggaactcgac
     2881 gaacgagcgg tcgtcgcgcc agccgagcgg agcggcgtcg gccatcacgt agtaagagcc
     2941 ctggggcgga gtgaacccga agcccgcttg ctcgatcgcg tcgagcagga tttttcgtcg
     3001 ggcgccgtac gctcgacgca gacgctcata gtagctgtcc ggcaggcgga gcgccgcccg
     3061 tcccgcctcc tggaacggcg cgggcgcgca gatggtcagg tagtcgtgga ccttgcggat
     3121 ccgtgacgtg agcgccggcg ccgcgacggc ccagccgacc cgccaccccg tgacggcgta
     3181 gctcttcccg agccccgaga gagtcaccgt ccggtcttcc attccgggca gcgagccgag
     3241 cgagacgtgc tgacggccgt cgtaccagat gtgctcgtag atctcgtcca cgatcgcgat
     3301 caccccggcg cgctcgcaga ggcgtccgac cgaggcgagc tcctcgcgcg tcaggacgcg
     3361 accggacggg tttcccggcg tgttgagcag caggacgcgg gtccgggggc ggatcgcttt
     3421 ctccagggcg tcgaggtcga gcgcgtagtc cggctccgcg agcgggacga agcgcgggcg
     3481 aacgccggcg aggacgcacg ccggaagata gttctcgtac cagggctcga ggatcagagc
     3541 ttcgtccccg ggctcggtga gcgcgagcac cgccgcgacc tgcgcctccg acacaccgca
     3601 cgtgatcgtc acctccgttt cggggtccgg ccgcatggag ttgaaccgcg cgtacttctc
     3661 cgccaccgca gcgcggaatt cgggcgaacc ccacgtgaac gaatactggt gcgcgcccgg
     3721 cttcgcgatc gcacgcgcca gcgccgccac gacggccggc gggggctcga agtcgggcag
     3781 cccctgcgcg agattgatgc cgcccacctc cgcgccgaaa cggctcatct cccggatcac
     3841 ggattcgccg aacgcgtccg tgcgccgcga gagagggcgt cggcgagccc gctgcaaccc
     3901 cacgacctta gactagcccg agcgacggct gatgaagacc acctcctcgg gcctgcaccg
     3961 ccggctgacc ctcgtcgagt acttcacgtt cggtttcggg accatggtgg gcgtcggctg
     4021 ggtcgtgctg atggacgact ggctgggtcg cggcggaccg gccggcgcct cgctcggctt
     4081 cctcgctggc ggccttctgc tcctcccgat cgcgcgaacc tatggacggc tcgtcgcccg
     4141 cttccccgac gccggaggcg agatcgcgta tgcggaaagc gtcttccctc cgccggcggc
     4201 cttcggagcc gcctggacga tggttctgtc gtacgcaatc gtgtgcccgt gggaggccgt
     4261 cgcggtcgga aacctcctcg cgcgcgtctt ccccgcgctg aacacgcttc ccctgtacga
     4321 ggtcgcgggc cgcgagatct acgcgccgcg gctcgccctg ggactcctcg tgaccgtcct
     4381 catcgccgcg atcaacgatc ggggggtccg gctctcgggc gtcttccagg acgcggcgac
     4441 cttcgggctc ctcgcgctct tcgtgctctt cgcggcgctc gcgttcgcgc gtggaagtcc
     4501 cggcaacctc ccgccgctct tcgcgcggcc gggtgtggcg ggggcctggc tctcggtcct
     4561 gatcgttctc caggtcgttc cgtacttcat gaccggtttc gagtcggtcg gcaaggggtc
     4621 ggaggaggcg catccggact ttccgccggc gcggttcggt cgcgcgatca cgcttgcggc
     4681 ggtctgcggg ttcctcttct acgcgacgat catcctcgcg gtagcgtgga tctttccctg
     4741 gcgggagctc gtgaccggac ggctggggac cgaggcggcg ttcgcacggg ctttcggatc
     4801 gccccttctc gcccgcctcg tcgtactcgc cgcgtttctc tcgctcgtga agatcttcaa
     4861 cgggaacttc ctcgccgcga cgcgaatgct ctacgggatc gcgcgacggg ggctcgtcca
     4921 cccgtcgctc gcgcgggtaa acactcgaaa tggaaccccc cgacgcgcca tcctcctcgt
     4981 ggcggggttc tccgccgtcg gagctttcct cggcgacgcc ctcctcgtgc cgatttccga
     5041 agtcggctcg ctcgccgtcg cggtggggtg gttcacgagc tgcgttgcgt gtgtcgcgct
     5101 ggcccgccgg ggggatgacc cctggcgcct tcccggtcgc cggcgagccg caatccgcac
     5161 ggccgcgatc ggcgcgctcg tcgccgcggc gatcatcgcg atgaaggtcc ttcccgtggt
     5221 tccgggcagc ttcactcggt gggagtgggt cgcattcctc gcctggtcct cgctgggatt
     5281 tggtttctgg ctctttcggc gcccggcacg accgatgctc tgaggtcgag cgggacggct
     5341 catggttgat tctgttcgaa gagctggcac tcgtttggca tttccggacg tcatgcctca
     5401 ccgggcgcgt gcccccttcg ggtgtctcct tgccgcggca ttcgcaatcg tgccttcggc
     5461 cgccttctcc gaggacctcg agcgccagat cgacagaatc caattcaacc tcctcgcgaa
     5521 cgcggcgctc gtgagccggg cgaccgccgg ggacatcgcc gttccccagt ggctgacggt
     5581 ctcgtttgca ggcaatgcga cgccgatcga agtcgtgact cccatcgcgc acggtctcac
     5641 gacgggagac gcggtgctca tcaagggcgt tctgggaaat gtcgcggcga acgggtggtg
     5701 gacgatcacg cgcacggcgg agacgaggtt cactctcgac ggatccgcag gcaacggtga
     5761 atacggcggc ggtggcgcgg tcttcccggc cgccgggcaa ccgcaacccg cgggtgcgcg
     5821 cggcgccggg ggcgagctgc cgtggacccc ctggttctcc gcccccgcag tggcgcgcga
     5881 gacggagttc tttcagccct tgggcgagct ctccacgttt ccctcggtcc cgccggtgaa
     5941 cagcttcctc tcgcaggaga tcgacggatc cctcttcgag ccgggcgagg ccctctgtct
     6001 ctcgatcgag gcgcgcatgc cggagtcggc gatcggcgac cagagactga agatggtggt
     6061 gacggcggcg ctcggcccga ctcgcgttta cagcacgacg tttcccgcca cgctcctgac
     6121 gccggtctac cagaggttcg cgctctgctt tcgcctggcg aatgacgccg ttccccgagg
     6181 cggcgttctg cgggtggagt tcatcgacga gcacctgcag gggatcccga agccgatgtt
     6241 ctggacgcgc ccgatgctga acgagggtgt cgcgccggcg ccctggacgg ccagcgtcga
     6301 gccgaggacg cgcacccgcg cgttccgttg actgttcggc gggatccgat cggcgcggga
     6361 gaagacttcg tgacatcgca gccgccggcg gtgcggtgat agaagtgcgc ccatgagaga
     6421 cctcgcgcag ggctgccggt ctctggtggt agtcgggatt ctcgcgtcca tcgcatcgct
     6481 tttctgcacg acgtccgttc gggggcaggg cgcactgccg atcgagcgga tcaagctccc
     6541 tcccggcttc gagatccggg tgtgggcccg ggcaccgggc gcccgctcga tgaccctctc
     6601 gcccggcggg actctgttcg tggggacccg ggatggaaac gtctacgcca ttcacgcggc
     6661 ttcgggcgga gagccgtcgg tcgtcactat cgcgcgcggg ctgaacatgc cgaacggcgt
     6721 cgcctttcgc gacggcgccc tctacgtcgc ggagatccat cgcgtgatcc gctacgacgg
     6781 aatcgaatcc cgcctctcaa acccgccggc accggccgtc gtgcgcgagt tcccgacgga
     6841 ccgccaccac ggctggaagt tcatcgcgtt cggcccggac ggcctgctct atgtccccgt
     6901 cggcgcgccg tgcaacatct gccagcccgc gccgatg
//
LOCUS       DASWAV010000306         1797 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U2665
            k141_176862, whole genome shotgun sequence.
ACCESSION   DASWAV010000306 DASWAV010000000
VERSION     DASWAV010000306.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960316
            Sequence Read Archive: SRR10260007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 1797)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1797)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:47:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,929
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1797
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_176862"
                     /isolate="SMAG_U2665"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.36444 N 132.31889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(183..259)
                     /locus_tag="VF958_06320"
     tRNA            complement(183..259)
                     /locus_tag="VF958_06320"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(223..225),aa:Pro,seq:ggg)
     gene            complement(323..877)
                     /locus_tag="VF958_06325"
     CDS             complement(323..877)
                     /locus_tag="VF958_06325"
                     /inference="COORDINATES: protein motif:HMM:NF024803.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HEX9148762.1"
                     /translation="MAQTTSENAEVRYKIGDVCRIADVQPYVLRYWESEFPVLAPDRS
                     VPGPRTYSARELDLIGQIKRLLYDEGYTIAGAKKRLENEASGARLHDTNPAVAPLEDA
                     KVEKSEKTSRPGRPSEKVPVRTRPSSKPGLPLFIETAPVGSTAPSAATGTDPAGAQAG
                     QTPDPRLVRVVAELKEILEILSRA"
     gene            complement(933..>1797)
                     /gene="der"
                     /locus_tag="VF958_06330"
     CDS             complement(933..>1797)
                     /gene="der"
                     /locus_tag="VF958_06330"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HEX9148763.1"
                     /translation="TGADELLEAIEEALPPDLDPDTRTEPLPIAIVGRPNVGKSSLVN
                     ALVGEDRVMVSEIAGTTRDSIDVRLSRGGREYVFVDTAGIRRKGKTTDSAELLSIVAA
                     RKSIDRARLVVIVFDASAGITSQDATIAGYAEEAGRGVLLVANKWDLVEKDHEKVSEL
                     KHDAERRFIFLRRAPFLPLSATTGKGVQRLLPEADALAKRFATRMSTGELNRLLQKSF
                     TREAPRSRSGRELKIRYAVQVATEPPLVRLFADRTEALHFSFERFLQNRIREKWNLNG
                     VPLKFVVKKDD"
BASE COUNT          270 a          595 c          612 g          320 t
ORIGIN      
        1 atgggacccg gacttgagga agacctcgac ggcccgagcg cacgccaccg ggttcgcttc
       61 gtgtgggacc tcgatcagga accgcgccgt catctgggat ctcctcctgc gatcggtgcg
      121 ccgcgccgga ccttgcggga gacgaaaatg gccgctcccg gcgcggactg atttgaaatc
      181 agtggtcggg acggcgggat tcgaacccgc gaccccttga accccattca agtacgctac
      241 caggctgcgc cacgtcccga ccgggaacga catcggagag tcgccgaggc gatctccggg
      301 gatggctgtt tatagcattt cgtcacgcgc gcgagaggat ctcgaggatc tccttgagct
      361 ccgcgacgac gcgcacgagg cgcgggtccg gtgtctgtcc tgcctgcgcc ccggccgggt
      421 ccgtgccggt tgccgccgaa ggcgccgtcg agccgacggg agccgtttcg atgaacagcg
      481 gcaggcccgg cttcgaagac ggcctcgtgc gcacgggcac cttctcggag ggccttcccg
      541 gcctcgacgt tttctccgac ttctcgacct tcgcgtcctc gagcggcgcg accgcgggat
      601 tcgtatcgtg caatctcgcc cccgaggcct cgttctcgag gcgcttcttg gcgccggcga
      661 tcgtgtagcc ctcgtcgtag agaagcctct tgatctgccc gatgagatcc agctcgcgcg
      721 cgctgtacgt gcgcgggccc ggaacggagc ggtcgggcgc gaggacggga aactccgact
      781 cccagtacct gaggacgtac ggctgcacgt ccgcgatgcg gcagacatcc ccgatcttgt
      841 agcggacctc ggcgttttcg ctcgtcgttt gggccatttc gtcagagttt cgcctgactc
      901 tttttagcac atcgagcggc cgatccgaag ggtcagtcgt cctttttgac cacgaatttg
      961 aggggcacgc cgttcaggtt ccacttctcg cggatgcggt tctggaggaa acgctcgaac
     1021 gagaagtgaa gcgcctcggt ccggtcggcg aagagccgga cgagcggcgg ctcggtggcg
     1081 acctgcacgg catagcggat cttcagctcg cggcccgagc gcgaacgggg cgcctcgcgc
     1141 gtgaaggatt tctgcagcag gcgattcagc tcgcccgtgg acatgcgtgt cgcgaaccgc
     1201 ttcgcgagcg cgtcggcctc gggaaggagg cgttgtacgc ccttccccgt cgtggccgac
     1261 agcggcagga acggggcgcg gcgcaggaag atgaaccggc gctcggcgtc gtgcttgagc
     1321 tccgacactt tctcgtggtc cttctcgacg aggtcccatt tgttggccac gaggaggacg
     1381 ccccggccgg cctcttccgc gtaccccgcg atcgtggcgt cctgcgacgt gattccggcg
     1441 gaggcgtcga agacgatgac gacgaggcgc gcccggtcga tggacttccg ggccgcgacg
     1501 atcgaaagca gctctgccga gtcggtcgtc ttccccttcc ggcggatgcc cgccgtgtcc
     1561 acgaagacgt attcgcggcc tccccgcgag aggcgcacgt cgatggagtc gcgcgtcgtg
     1621 ccggcgatct ccgacaccat gacccggtcc tcgccgacga gcgcgttcac gagggatgac
     1681 ttcccgacgt tcggccggcc cacgatcgcg atcgggagcg gctccgtgcg cgtgtcggga
     1741 tcgaggtcgg gaggcagggc ctcctcgatc gcctccagaa gctcgtccgc gccggtg
//
LOCUS       DASWAV010000307         3102 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U2665
            k141_374537, whole genome shotgun sequence.
ACCESSION   DASWAV010000307 DASWAV010000000
VERSION     DASWAV010000307.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960316
            Sequence Read Archive: SRR10260007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 3102)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3102)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:47:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,929
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3102
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_374537"
                     /isolate="SMAG_U2665"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.36444 N 132.31889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1674)
                     /gene="dnaK"
                     /locus_tag="VF958_06335"
     CDS             complement(<1..1674)
                     /gene="dnaK"
                     /locus_tag="VF958_06335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014100380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HEX9148764.1"
                     /translation="MSKIIGIDLGTTNSCVAVMEGGDPVVIPNAEGNRTTPSVVAFSK
                     SGERLVGQVAKRQAITNPANTVFSIKRFMGRKSSEVTEEMKMVPFKVVSAPNGDVRVA
                     AGGKEYSPPEISAMILGKLREAAEAYLGQKVTRAVITVPAYFNDSQRQATKDAGQIAG
                     LTVERLVNEPTAAALAYGLDKKKDETIAVYDFGGGTFDISILEVGEGVVEVKSTNGDT
                     HLGGDNIDQKVIDWILAEFKKDQGIDLSKDPMAMQRLKEAAEKAKIELSNVVETDVNL
                     PFITADASGPKHLQLKLTRSKLEQLVEEIVNRSIAPCRQALKDAGLEPKDIDEVVLVG
                     GQTRMPRIQQLVRELFGKDPHQGVNPDEVVAVGAAIQGGVLAGDVKDLLLLDVTPLSL
                     GIETLGGVTTKLIERNTTIPTKKSEVFSTAADNQTSVEIKVLQGEREMARDNKPLGVF
                     HLIGLPPAPRGMPQIEVTFDIDANGILNVSAKDLGTGKEQKITITASSGLAKDEIDRM
                     VSDAQSHSEEDRKRREEIEARNRLDSLVYSTEKTLGENRDKIDPSEISTF"
     gene            complement(1785..3041)
                     /locus_tag="VF958_06340"
     CDS             complement(1785..3041)
                     /locus_tag="VF958_06340"
                     /inference="COORDINATES: protein motif:HMM:NF025690.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4388 domain-containing protein"
                     /protein_id="HEX9148765.1"
                     /translation="MSLDGNLRVFSLTEVFQMLGMQHKSGVLTVEGPKDTISVGFVTG
                     RIVSVESSSNRIENRLGTLLVKAGRLSEDELAGALELQKKKQGRLGRLLLTEEIVRAE
                     DLREAFRIQVQRILFAAFGWTDGRFRFAPQAVIEHDPEIFQPMPTESILLEAARNFDE
                     LPLAEQKIPSNELVFRRASAAHALRLVVGSASDEEGAVAVSKREAETWKWIDGRRTVA
                     EIRERAFLSDLDVLKGISDLLDRGLIEEGYLRETPSSAEAVRRRTLPITTILPWIPVI
                     AAAAVAVSFAPRNPANVLLQPVSQNRPARDLLKANSLARLLTVGRAVRVYYGASGRYP
                     RTLGQIVAQGILGEDSLQDPYGRFYRYILRPEDGKFALYGRSANGQIDLDLAHEGQLA
                     PTAEVRPAGTPLTPPPERPTGVRVVD"
BASE COUNT          490 a         1000 c         1001 g          611 t
ORIGIN      
        1 gaacgtcgag atctccgacg gatcgatctt gtcccggttc tcgcccaagg tcttttcggt
       61 ggagtagacg aggctgtcga gacggttgcg tgcctcgatc tcctcccggc gcttgcgatc
      121 ctcctccgag tgagactggg catcggagac catgcggtcg atctcgtcct tcgccaggcc
      181 ggacgaggcg gtgatcgtga tcttctgctc cttcccggtg ccgaggtcct tcgcggagac
      241 gttcaggatg ccgttcgcgt cgatgtcgaa cgtgacctcg atctgcggca taccgcgcgg
      301 cgccggtgga agcccgatca ggtggaacac gccgaggggc ttgttgtcgc gcgccatctc
      361 gcgctccccc tggagaacct tgatctcgac ggatgtctgg ttgtcggccg ccgtcgaaaa
      421 gacctcgctc ttcttcgtgg ggatcgtcgt gttgcgctcg atgagtttcg tcgtgacgcc
      481 gccgagcgtt tcgattccga gcgagagagg ggtgacgtcg agaaggagga ggtccttcac
      541 gtctcccgcg agaacgcccc cctggatcgc cgcgccgacc gcgacgactt catcggggtt
      601 cacgccctgg tgcgggtcct tgccgaagag ctcgcggacg agctgctgga tgcgcggcat
      661 gcgggtctga ccgccgacga gaacgacctc gtcaatgtcc ttcggctcga ggccggcgtc
      721 cttcaacgcc tggcggcagg gtgcgatgga acggttcacg atctcttcga ccagttgctc
      781 gagcttcgac cgagtgagct tcagctgcag gtgcttcggc ccggaggcat ccgccgtgat
      841 gaacgggagg ttgacgtccg tttccacgac gttcgagagc tcgatcttcg ctttctccgc
      901 ggcctccttc aaacgctgca tcgccatggg gtccttcgag aggtcgatgc cctggtcctt
      961 cttgaactcg gcgaggatcc agtcgatgac cttctggtcg atgttgtcgc ctcccaggtg
     1021 agtgtcgccg ttggtcgact tgacctcgac gacgccttcc cctacctcga ggatcgagat
     1081 gtcgaacgtt ccgccgccga agtcgtagac ggcgatcgtt tcgtccttct tcttgtcgag
     1141 gccgtaggcg agcgcggcgg ccgtcggttc gttgacgagc cgctcgaccg tcaatccggc
     1201 gatctggccg gcgtccttgg tcgcctggcg ctgagagtcg ttgaaatacg cggggaccgt
     1261 gatcacggcg cgcgtgacct tctgtccgag atacgcctcg gccgcttccc tcagcttgcc
     1321 gaggatcatc gcggagatct cgggaggtga atactcctta cccccggcgg caacacgcac
     1381 gtcgccgttg ggcgcggaga ccaccttgaa gggcaccatt ttcatttcct cggtgacttc
     1441 cgaggacttg cgtcccatga agcgcttgat cgagaagacg gtgttcgccg ggttcgtgat
     1501 cgcctggcgc ttggcgacct ggcccacgag gcgctcgcca ctcttggaaa acgcgacgac
     1561 cgacggagtt gtccggttcc cttccgcgtt ggggatgacg accgggtctc ccccctccat
     1621 cacggccacg cacgagttcg tggtgccgag gtcgattccg atgatcttgc tcatttccct
     1681 ctcctcgcct tctgaaatag gcgcccatcg taggacttta gcgtccccgt gtcaagttcc
     1741 gaacagcaaa tcctaagcca cctcgactca ggtgctcgtc cggcctaatc gactactcgt
     1801 acccctgttg gccgctcagg aggaggcgtc agcggggttc cggcggggcg aacctcggcg
     1861 gtgggggcga gctggccctc gtgagcgagg tcgaggtcga tctgcccgtt cgcgctgcgc
     1921 ccgtagaggg cgaacttgcc gtcctcgggt cgaaggatgt agcgatagaa gcgcccgtac
     1981 gggtcctgca gcgagtcctc gccgaggatt ccctgggcaa cgatttgtcc gagcgtccgc
     2041 ggatagcggc cggacgcacc gtaatacacg cgcactgccc gccccaccgt gagcaggcgc
     2101 gcgagagagt tcgccttcaa caggtcccgc gccggccgat tctgcgacac cggctgaagt
     2161 aacacgtttg cgggattgcg cggcgcgaag gagacggcga ccgcggcagc tgcgatcacc
     2221 gggatccacg ggaggatcgt ggtgatcgga agcgtgcggc ggcggaccgc ctccgccgaa
     2281 ctcggcgtct cccgaagata gccctcctcg atcaggccgc gatcgagcag gtcggagatt
     2341 cccttcagga cgtccaggtc ggagaggaac gcgcgctcgc ggatttctgc gaccgttcgg
     2401 cgtccgtcga tccacttcca cgtctccgct tcccgtttcg aaacggccac cgcgccctct
     2461 tcgtccgaag cgctcccgac aacgagacgc agcgcgtgcg cggccgaagc gcggcggaag
     2521 acgagctcgt tcgacgggat cttctgctcg gcgagcggca gttcgtcgaa gtttcgcgcg
     2581 gcttcgagaa ggatcgactc ggtcggcatc ggttggaaga tctccggatc gtgctcgatg
     2641 acggcctgag gcgcgaaccg gaagcgtccg tcggtccagc cgaacgcggc gaacaggatc
     2701 cgttggacct ggatgcggaa agcctcgcgc agatcctcgg cacgcacgat ctcctcggtt
     2761 agaaggagcc ggccgaggcg tccctgtttt ttcttctgga gctcgagagc gccggcgagc
     2821 tcgtcctccg acagcctgcc cgctttcacg aggagcgtgc cgagccggtt ttcgatccgg
     2881 ttcgagctcg actcgacgga gacgattcgt cccgtcacga acccgacgct gatggtgtcc
     2941 ttcggtccct cgaccgtcag gacgccgctc ttgtgctgca tgccgagcat ctggaagacc
     3001 tcggtgagcg agaacacgcg aaggttcccg tcaaggctca cgggtcgtcc tccatgcacc
     3061 gacgatccct ccgaaccaca gggcgagcgc gaacgcgccg ag
//
LOCUS       DASWAV010000308         2234 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U2665
            k141_96617, whole genome shotgun sequence.
ACCESSION   DASWAV010000308 DASWAV010000000
VERSION     DASWAV010000308.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960316
            Sequence Read Archive: SRR10260007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2234)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2234)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:47:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,929
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2234
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_96617"
                     /isolate="SMAG_U2665"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.36444 N 132.31889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            224..1210
                     /locus_tag="VF958_06345"
     CDS             224..1210
                     /locus_tag="VF958_06345"
                     /inference="COORDINATES: protein motif:HMM:NF024660.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PHP-associated domain-containing protein"
                     /protein_id="HEX9148766.1"
                     /translation="MASKRAEEPERPGWFSDGRFKKADLHCHSVYSTFKYFRIANTRD
                     SYNRPEDVYRLAKQRGMDFVTLTDHDSIDGCLYLLNKFPDLDDFFISEEVETWFPDTR
                     QRIHINVFAIDERQHAEIQKRRENIYDLHAYIREENILASANHMFQNYRMRNSPRQYF
                     DEMLRMFDAFEVKNGAMASQHNRLVEDLMAVVREKRGDVSLVAGSDAHTLGPLARVYT
                     VAEAETVPEFLEKVRLGQCFVWGSEMGFRVLLSDVYRMVFRYYGSVLDLKNPEFTRGE
                     KARHLALAAVGAPITAAGVPLAITSLNYVKQIFVTRRIGHELMSHLGDEKPS"
     gene            1283..2149
                     /locus_tag="VF958_06350"
     CDS             1283..2149
                     /locus_tag="VF958_06350"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020249365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016888 - endodeoxyribonuclease activity,
                     producing 5'-phosphomonoesters [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HEX9148767.1"
                     /translation="MAILDLPLGAHQSIAGGTPRAIERGVEVGCRVLQIFVKNNNRWV
                     GKPIERPEARAFRSAARGANLSRVIAHTSYLINLASPVDELRRQSIDALVEEIERCRR
                     LGIPDLVYHPGSHGGEGEAAGVRRIASSLDEVFARTEGARVRILLETAAGQGSCVGHR
                     FEHLRDVLAAVRNPRRVAACFDTCHVHAAGYDIVSREGWLETLSAFDRTVGLARLAAI
                     HVNDSKKPRGSRVDRHEHIGQGTIGRRGFRNLMTDPRLASIPKFLETPKDEDTLLQDR
                     RNLAVLRRLATT"
BASE COUNT          375 a          754 c          711 g          394 t
ORIGIN      
        1 ttgctcggca tctggcttcg ccaccggcgc ccggctcggc aagccggcct cgaccgggcg
       61 cctgatgcct tgcggcgctt gcgctcgcgc ggcgcaaagc tcctccgctc ggttacttcg
      121 cggagactcc tcgcgccgcg aaggcccgaa gcggttcgga tcgcccgata tttcacacct
      181 gcttgactaa ccggcctgcg ctttgcacac tcacgtttct ctgatggcgt cgaaaagggc
      241 tgaagagccg gaaagacccg gctggttctc ggacggtcgc ttcaagaaag cggacctgca
      301 ctgccactcg gtgtactcga cgttcaagta tttccggatc gccaacacgc gcgactcgta
      361 caaccgtccc gaggacgtct atcgcctcgc gaagcagcgc gggatggact tcgtgacgct
      421 gaccgatcac gactcgatcg acggctgtct ctacctgctc aacaagttcc cggacctcga
      481 cgacttcttc atctcggaag aggtcgaaac gtggttcccc gacacgcgcc agcgaattca
      541 catcaacgtc ttcgcgatcg acgaacggca gcacgccgag atccagaagc ggcgggagaa
      601 catctacgat ctccacgcct acatccgcga agagaacatc ctcgcgtccg cgaaccacat
      661 gttccagaac tacaggatgc ggaactcccc gcggcagtac ttcgacgaga tgctgcggat
      721 gttcgacgcg ttcgaggtca agaacggcgc gatggcgtcc caacacaacc ggctcgtcga
      781 ggacctcatg gccgtcgtgc gcgaaaagcg tggcgacgtg tcgctggtcg cgggctccga
      841 cgcgcacacg ctgggacctc ttgcccgcgt ctacaccgtc gcggaagccg agaccgttcc
      901 cgagttcctc gagaaggtcc ggctcggcca gtgcttcgtg tgggggagcg aaatggggtt
      961 ccgcgttctc ctctccgacg tctatcgaat ggtcttccgc tactacggca gcgtgctcga
     1021 cttgaagaat ccggagttca cgcgcggcga gaaagcgcgg cacctcgctc tggccgccgt
     1081 cggcgcgccg atcaccgcgg ccggagtgcc gctcgcgatc acgtcgttga actacgtgaa
     1141 gcagatcttc gtgacgcggc gcatcgggca cgagttgatg tcgcacctgg gagacgagaa
     1201 gccgtcgtag ggggcttccc cctctccctt tgggagaggg tcgggggtgt gggcgttgag
     1261 tcgccacgct tagaattcgc gcgtggccat tctcgatctc cctctcggcg ctcatcagtc
     1321 gatcgcgggc ggcactccgc gagcgatcga gcggggggtg gaggtcggct gccgcgtcct
     1381 gcagattttc gtcaagaaca acaaccgctg ggtcggaaag ccgatcgagc gtccggaggc
     1441 gcgcgcgttc cgctccgcgg cgcggggggc aaacctttcc cgggtcatcg cgcacacttc
     1501 gtacctgatc aacctcgcct cgccggtcga cgagctccgc cggcaatcca tcgacgccct
     1561 cgtcgaagag atcgagcgct gccggaggct cggcatcccc gatctcgtct accaccccgg
     1621 ttcgcacggc ggtgaaggcg aggccgccgg agtccggcgg atcgcgtcat cgctcgacga
     1681 ggtgttcgcg cggaccgaag gagcgcgcgt gcgcattctt ctcgagaccg ccgcgggtca
     1741 gggatcctgc gtcggtcacc gcttcgagca cctccgcgac gtcctcgccg cggtgcgcaa
     1801 cccgcgccgc gtcgcggcgt gcttcgacac ctgccacgtg cacgcggccg gatacgacat
     1861 cgtgagccgc gagggctggc tcgaaacgtt gagcgcattc gaccggaccg ttgggcttgc
     1921 ccgcctcgcc gcgatccacg tcaacgactc gaagaaaccg cgcggctcgc gcgtcgaccg
     1981 gcacgagcac atcggccagg gcacgatcgg gcggcgcggg ttccgcaatc tcatgaccga
     2041 cccgcggctg gcgtcgatcc cgaagtttct cgagacgccg aaggacgagg acactctcct
     2101 gcaggatcgg agaaacctgg cggtgctgcg gcggctcgcg acgacgtgag gagagccgta
     2161 ctcctctctc ttgccgggtc ggccgtcctg acagtgtttt cgatcgccgc gcttccgttt
     2221 tcgcggggcc tgtt
//
LOCUS       DASWAV010000309         3045 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U2665
            k141_55406, whole genome shotgun sequence.
ACCESSION   DASWAV010000309 DASWAV010000000
VERSION     DASWAV010000309.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960316
            Sequence Read Archive: SRR10260007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 3045)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3045)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:47:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,929
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3045
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_55406"
                     /isolate="SMAG_U2665"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.36444 N 132.31889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1084
                     /locus_tag="VF958_06355"
     CDS             <1..1084
                     /locus_tag="VF958_06355"
                     /inference="COORDINATES: protein motif:HMM:NF024110.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX9148768.1"
                     /translation="PGSRGIHWVVETRPITGDLERTKEMLRKEVLEKKIYGYLVLDPA
                     LLEKDRAEYYSISVSEFVALTQLEQAINKVLLRRKIEDRGLPASLGKELERRVDLKTL
                     KVTTKGTTEEKGAGILAAIIFMVIMYMTFFMYGFQNLRGVIEEKTNRIVELVIASVRP
                     TELMLGKIIGIGLVGLTQYLIWSLVAMNLSLPGIASLMAMGDSGAPTIPLSMIGYFLL
                     FFLLGYFLYASIYTAIGAPFNTDQEAQQLSMIPSMLIIGGMAVYPSVMNSPNGPVAVF
                     ASLFPFTAPLIMYLRTAIAEPPVWQIALCIVILILSTIGMAWIAGRIYRVGILMYGKK
                     PTLPEILRWIRYRPGKVLQPAAPPAN"
     gene            complement(1160..1753)
                     /locus_tag="VF958_06360"
     CDS             complement(1160..1753)
                     /locus_tag="VF958_06360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012111109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9148769.1"
                     /translation="MKRDQLNELVYQMLETEMGGVEIYRTAVRCATNDDLKKEWKKYL
                     GQTENHVEVVRELMSELGLDPEADTPGRDVVRHVGKSLVKAMEMALTSAPREAAQIVA
                     AECVVLAETKDHLNWELLGQVAKKIKGEEKKALEAAQEKVEDEEDEHLYHTAGWAREL
                     WIEFLGMPAVIPPPEEEKDVKTAIGAARAKQARAEML"
     gene            1955..2029
                     /locus_tag="VF958_06365"
     tRNA            1955..2029
                     /locus_tag="VF958_06365"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1988..1990,aa:Glu,seq:ctc)
BASE COUNT          488 a         1066 c          894 g          597 t
ORIGIN      
        1 accggggtcg cgcgggattc actgggtcgt cgagacacgc ccgatcacgg gcgatctcga
       61 acgcacgaag gagatgctgc ggaaggaagt cctcgaaaag aagatttacg gctatctggt
      121 tctcgacccg gcgctcctcg aaaaggaccg cgccgagtac tactcgatct cggtctcgga
      181 attcgtcgcg ctcacgcagc tcgagcaggc catcaacaaa gtcctcctgc ggcgcaagat
      241 cgaggaccgg ggactccccg cctcgctcgg taaggagctc gagcggcgcg tggacttgaa
      301 gaccctgaag gtcacgacca agggaaccac cgaggaaaaa ggcgccggga tcctcgcggc
      361 gatcatcttc atggtgatca tgtacatgac gttcttcatg tacgggttcc agaacctgcg
      421 cggggtgatc gaagagaaga cgaatcgcat cgtcgagctc gtgatcgcat cggtgcggcc
      481 gacggaactg atgctcggta agatcatcgg tatcgggctg gtcggcttga cccagtacct
      541 gatctggtcg ctcgtcgcga tgaacctgtc gcttcccggg atcgccagcc tcatggcgat
      601 gggcgattca ggggcgccga cgatcccgct ctcgatgatc ggatacttcc tgctgttctt
      661 cctgctcggt tacttcctct acgcaagcat ctacacggcg atcggagcgc ccttcaacac
      721 cgaccaggaa gcgcagcagc tctcaatgat tccgtcgatg ctgatcatcg ggggaatggc
      781 ggtttaccct tcggtcatga acagcccgaa tgggcccgtc gccgtgttcg cgtccctctt
      841 tccgttcacg gcgcccctca tcatgtacct gcgaacggcg atcgccgagc cgcccgtctg
      901 gcagatcgcc ctgtgcatcg tgatcctcat tctgtcgacg atcggaatgg cgtggatcgc
      961 cggacggatc taccgcgtgg gaatcctgat gtacggaaag aaacccacgc tgcccgagat
     1021 cctgcgctgg atccgctacc ggccgggtaa ggtgctccag ccggccgcgc cgccggcaaa
     1081 ctagctcccc tctcgaggag ctatcccaca tgcctccctc tccccgctcg cggagagagg
     1141 gtttgggtga gggttctccc tagagcatct ccgcgcgtgc ctgcttcgcg cgggcggcgc
     1201 cgatcgcggt cttcacgtcc ttttcctctt cggggggcgg aatgaccgcg ggcatcccga
     1261 ggaactcgat ccagagctcc cgcgcccagc cggcggtgtg gtacaggtgc tcgtcctctt
     1321 cgtcttcgac cttctcctgc gccgcttcga gcgccttctt ctcctcgccc ttgatcttct
     1381 tcgcgacctg accgagcagc tcccagttca ggtggtcctt cgtctcggcg agcaccacgc
     1441 actccgcggc tacgatctgc gcggcctcgc gtggagcgga cgtcagcgcc atctccattg
     1501 ccttgaccag agacttgccc acgtggcgaa cgacgtcgcg accgggcgtg tcggcttcgg
     1561 gatcgagtcc gagctcactc atgagctcac ggacgacctc gacgtggttc tcggtctgtc
     1621 cgagatattt cttccactct ttcttcaggt cgtcgttcgt cgcgcaccgg accgcggtgc
     1681 gatagatctc gacgcccccc atctcagtct ccaacatctg gtaaacaagt tcattcaact
     1741 gatctctctt catggttcct cctcgcggca ccgctcaatg caaggcccgg gccgccgtcc
     1801 gtcgccgtcg ttcgctcggg atctggctgg cgccacccgc acgcggctcg gcagaccggc
     1861 ctcgaccgcc tgcctgatcc ctcgctcact cccgcgacca gagcccccgg gcttgccgcg
     1921 aaaaccgcct ccgtgctact ttcccgcacc cgtcggccca ttcgtctaac ggtcaggacg
     1981 cagccctctc aaggctgaaa tacgggttcg attcccgtat gggctaccaa gaacgtcgcg
     2041 gtcgcttgct cggtcgctgc tgaggcaccg gcccgcggct cgccaacccc ggcgcgaccg
     2101 cttgcctgct ccctcgcggc gcgcccgctc ccgcagcgtg aggctcctcc cttcgggaga
     2161 ctcctcacgc tgcgaccagg accctcggga gactcctcgc gccgcgacta tgcctctcag
     2221 gagactccgt ccgcgataaa tgcccctaat cgaggctccg ccagggcgga gagttggtgg
     2281 gcatgggttg gcgggaactt cgagtctgcc attaagtacg aaggaacggg gcaggtcgtt
     2341 tctttcgtcg aacccgtcgc tcgattcccc cggacgtcgg aatcgaagtc ggggcgcgac
     2401 ccccgcggcg ggggcgccgc ggttccgacg atcccctcga ggaggccgtc ctccagggac
     2461 agcttctcgt ccgagaacgg aattcgcccc aacacaacgg aattacgagg ttcatccgat
     2521 gccgaaacag cggacattca cccgccgcac gctcctgacc tccctcgcca cggccggcgc
     2581 tgtttcagtt ctccgccctc tcgcgccgac ggccgcgcgg tctcccatcc tcacccgccc
     2641 gatcctctcg agcggcgaga agctgccgct cgtcgggctc ggcagctgga tcacgttcaa
     2701 cgtcggcgac gacgtcgctg cccgcaacgc ccggaccgaa gtcctgcgcg cgttcctcga
     2761 cggcggcggg cgcctgatcg actcctctcc gatgtacggc tcgtcgcagg aggtgcgcgg
     2821 cacggatgca tccggtcctg ccgggaccgt taccacggtc ccgccggttg acttactgtt
     2881 agcgggtcgt gagctcgacc gccgatctcc ccgcgccgcg caagtcgcgc gtccgccgga
     2941 acaaggtctg gaacctcgtc ctcgcgatct tctttttcat cctcggggtc atcggcatct
     3001 tcataccggt gatgccgcag gtcatcttct tcgtgatgag cctcc
//
LOCUS       DASWAV010000310         1514 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U2665
            k141_296524, whole genome shotgun sequence.
ACCESSION   DASWAV010000310 DASWAV010000000
VERSION     DASWAV010000310.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960316
            Sequence Read Archive: SRR10260007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 1514)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1514)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:47:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,929
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1514
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_296524"
                     /isolate="SMAG_U2665"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.36444 N 132.31889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..193)
                     /locus_tag="VF958_06370"
     CDS             complement(<1..193)
                     /locus_tag="VF958_06370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9148770.1"
                     /translation="MFRKLALAVCAAIFIGLAVFAVTLPDVRPLAKSFPSTTAFMERR
                     RASLAQSGLSTRLEWTPVPI"
     gene            complement(211..>1514)
                     /gene="lat"
                     /locus_tag="VF958_06375"
     CDS             complement(211..>1514)
                     /gene="lat"
                     /locus_tag="VF958_06375"
                     /EC_number="2.6.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014066340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0045484 - L-lysine 6-transaminase activity
                     [Evidence IEA];
                     GO_process: GO:0017000 - antibiotic biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="L-lysine 6-transaminase"
                     /protein_id="HEX9148771.1"
                     /translation="VLDVLSRHILVDGYHVVMDLERSRGSYLYDSRSDRLLLDFFTNF
                     ATYPVGYNHPKTADPEYRERILLAALNKPTNSDTYTTLYAEFVETFSRLAVPPTHGRH
                     LFFVEGGSLAVENAVKTAFDWKVRKNFARRIAEERGHQILHFRHAFHGRSGYSISMTN
                     TADPRKTQYFPKFDWPRLTCPKLSFPVTADVLRDVAEAEDSVEREIREACARFPNDIA
                     ALIIEPIQGEGGDNHFRPEFFARLRRLADELEFLLVFDEVQTGVGLTGSMWAWQQFGV
                     EPDLFCFGKKTQVCGFASNGRVDEVENVFTISSRINSTWGGNLADMVRCGKYLEIITE
                     ENLADNARVVGEHLVGRLRELAEELPVISNVRGRGLFVALDLPDKETRDRTLSACLEN
                     GLIALASGAAAIRFRPALNLSRDEADEGVRKLRRAVASTER"
BASE COUNT          270 a          468 c          532 g          244 t
ORIGIN      
        1 cgatcgggac gggagtccat tcgaggcgcg tggacagtcc cgactgcgcg agagaggcgc
       61 gccgcctctc catgaacgcc gtcgtactcg gaaagctctt cgcgagcggg cggacgtccg
      121 gaagggtgac ggcgaagacc gccaggccga tgaagatcgc cgcgcacacg gcgagcgcga
      181 gcttgcgaaa cacggtcgtc ggagagaagt ttagcgctcg gtcgacgcga cggcgcggcg
      241 gagcttgcgc acgccctcat cggcctcgtc gcgcgagagg ttcagagccg ggcggaaccg
      301 gatcgccgcc gctcccgaag cgagcgcgat caggccgttc tccaggcacg cggaaagcgt
      361 tcggtcgcgc gtctccttgt cgggcaggtc gagggcgacg aagagaccgc gccctcggac
      421 gttcgaaatc accggcagct cctcggcgag ctcgcgcagc cgaccgacca ggtgctcgcc
      481 gacgacccgg gcgttgtccg cgagattctc ctcggtgatg atctcgaggt acttcccgca
      541 gcggaccatg tcggccagat tgccgcccca cgtcgaattg attcgcgacg agatcgtgaa
      601 gacgttctcg acctcgtcca cccgcccgtt ggacgcgaag ccgcacacct gggtcttttt
      661 tccgaagcag aagaggtcgg gctcgacgcc gaactgctgc caggcccaca tcgagccggt
      721 cagtccgacc ccggtctgca cctcgtcgaa gacgagaagg aactcgagct cgtcggcgag
      781 acgccgcagg cgcgcgaaga actccggacg gaagtggttg tcgccgccct ccccctggat
      841 gggctcgatg atgagagcgg cgatgtcgtt cggaaagcgg gcgcaggcct cgcggatctc
      901 ccgctcgaca ctgtcctcgg cttcggccac gtcgcgcagg acgtcggccg tgacgggaaa
      961 cgacagcttc gggcaggtga gccgcggcca gtcgaacttc ggaaagtact gagtcttccg
     1021 gggatcggcc gtgttcgtca tcgagatcga gtatccggac cggccgtgga aggcatggcg
     1081 gaagtggagg atctgatggc cacgttcctc cgcgattctg cgcgcgaaat tcttccggac
     1141 cttccagtcg aaggcggtct tcacggcgtt ctcgaccgcg agggagccgc cctcgacgaa
     1201 gaagaggtgg cggccgtgcg tcggcggcac ggcaagcctc gagaaggttt cgacgaactc
     1261 ggcgtagagc gtcgtgtagg tgtcgctgtt ggtcggctta ttgagcgccg cgagcaggat
     1321 ccgctcccga tactcgggat ccgccgtctt cgggtggttg tagccgaccg ggtaggtcgc
     1381 aaagttggtg aagaaatcga ggagcagccg gtcgctgcgc gagtcgtaga gatacgagcc
     1441 gcggctccgc tcgaggtcca tgacgacgtg gtagccgtcg accaggatgt gacgggaaag
     1501 gacgtcgagg acgt
//
LOCUS       DASWAV010000311         2307 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U2665
            k141_417357, whole genome shotgun sequence.
ACCESSION   DASWAV010000311 DASWAV010000000
VERSION     DASWAV010000311.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960316
            Sequence Read Archive: SRR10260007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2307)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2307)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:47:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,929
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2307
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_417357"
                     /isolate="SMAG_U2665"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.36444 N 132.31889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..311
                     /locus_tag="VF958_06380"
     CDS             <1..311
                     /locus_tag="VF958_06380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9148772.1"
                     /translation="LSKLQTRNQEAGLRVIGVSTDFPKERAAVEKFLVDSRPSFPSYL
                     KKSGGDDEDFINAVDSSWGGELPFSVLYDRAGKKVHVYSGSLPLAAAEKEIRRLLAAR
                     "
     gene            complement(308..790)
                     /locus_tag="VF958_06385"
     CDS             complement(308..790)
                     /locus_tag="VF958_06385"
                     /inference="COORDINATES: protein motif:HMM:NF019301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family membrane protein"
                     /protein_id="HEX9148773.1"
                     /translation="MNSRSSKGVAVVRILTGVVFIAEGWAKTAGAFVRGGFAESVRET
                     ASGKAWPFWSSFLHRVVAPNAGAFAWFFALAELALGLALLVGVLTRAAAAGGIVLMLI
                     LLLGQTNVAGSSWDKWVTAGLTAKFALLLLWLLLLADAGRVWGLDGRLRKGGHPARAR
                     "
     gene            940..1956
                     /locus_tag="VF958_06390"
     CDS             940..1956
                     /locus_tag="VF958_06390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9148774.1"
                     /translation="MKRKSFGPVAVLSSAVALSLLASAAFAGQTHVVSRAGNVRPEAA
                     SAAAVSYSVEVPVVTRVVGTALFRTAIDISNNTSSGTSAAPVVAKLQYSYSRVSSDGT
                     VSFFRTPDPNSSQGQAMRINLLAFDNFHTDDFVQYLGTLPGVLQPGADQSSFGTLLIT
                     FEGLPSSQGWEGTVFARTYSPNPTGAGTVAIAYPASLFFESANQSLVATIRDTQTAPS
                     VAGTLRTNIGIRNTDIRGTAQTVSVQLSFYDVTPGNATNGQRVGGVLTLSNLQPGEVR
                     QLNNIRALAAIPDNVVGMLAFADVIGASASSPTIEGYVNILDGGTQDGAFFEMKCSDT
                     DGCG"
     gene            complement(1979..>2307)
                     /locus_tag="VF958_06395"
     CDS             complement(1979..>2307)
                     /locus_tag="VF958_06395"
                     /inference="COORDINATES: protein motif:HMM:NF014856.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009113 - purine nucleobase biosynthetic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="phosphoribosylglycinamide synthetase C
                     domain-containing protein"
                     /protein_id="HEX9148775.1"
                     /translation="SPLEVKAEVCAGVVLCSAGYPGVSVGGKAITGLTEAARQPGVQL
                     FHAHTEREGDRLMAGGGRVIVVTATAPSLSEALGRAYEAAERVEFEGKHFRRDIGAAW
                     LRGKRA"
BASE COUNT          404 a          824 c          708 g          371 t
ORIGIN      
        1 aactgtcgaa gctgcagacg cggaaccagg aggcgggact ccgggtgatc ggcgtttcga
       61 ccgacttccc gaaggagcgg gccgcagtgg agaagtttct ggtcgatagt cgtccctctt
      121 tcccgagcta cctcaagaag agcggcggcg acgacgagga cttcatcaat gccgtggatt
      181 cgtcgtgggg cggcgaactc cccttttccg ttctttacga ccgcgccggg aagaaagtcc
      241 acgtttactc gggttctctg ccgctcgccg cggccgagaa agaaatccga aggctcctcg
      301 cagcccgcta acgcgcacgg gcgggatgcc cgcccttccg gagccgcccg tccagccccc
      361 agacgcgtcc cgcgtcggcg agaagcagga gccagagaag cagaagagcg aacttcgccg
      421 tgagtcccgc ggtgacccac ttgtcccagc tcgaccccgc gacgttcgtc tggccgagca
      481 gcaggatgag catcagcacg atccctcccg cggcggccgc gcgggtgagc acgccgacga
      541 ggagagcgag accgagcgcg agctcggcca gcgcgaagaa ccatgcgaac gcgcccgcgt
      601 tgggagcgac gacccggtgg agaaaagagc tccagaacgg ccaggccttt cccgacgcgg
      661 tctcccggac cgattcggcg aaacccccgc ggacgaaggc gccggccgtc ttcgcccacc
      721 cttccgcgat gaagacgaca cccgtcagaa tccgcacgac cgcgacgcct ttcgaggagc
      781 gggagttcat ccccgcagtc taggatggcg gccccggatg tagggcccct gtcttttcgt
      841 cggccctcga aaccgattct ggatcgggaa ttgcttcaat gtgcccggcg gttctgggtt
      901 cctgtgcggg cccacacccc ggaacaggag atgactcaca tgaaacgcaa gtcgttcggt
      961 cccgtggcgg tcctatcgtc cgccgtcgcg ctctcgctcc tggcgtcggc cgcctttgcc
     1021 ggccagactc acgtcgtcag tcgcgccggg aacgtccggc cggaggcggc atccgctgcc
     1081 gccgtctcct actccgtcga ggtcccggtc gtcacgcggg tcgtcggcac cgcgctcttc
     1141 cggactgcga tcgacatctc caacaacacg agctccggca cgtcggcggc gcccgtcgtc
     1201 gccaagctcc aatactcgta cagccgcgtc agttcggacg ggaccgtcag cttctttcga
     1261 acccccgacc ccaactcgtc gcagggccag gcgatgagga tcaacctgct cgcattcgac
     1321 aacttccaca ccgacgattt cgttcaatac ctcgggacgc tccccggagt ccttcagccg
     1381 ggtgccgatc agagctcgtt cggaactctt ctcatcacct tcgagggtct gccctcgtct
     1441 caggggtggg agggaacggt cttcgcgcga acgtacagtc ctaacccgac cggcgccggc
     1501 accgtcgcga tcgcatatcc ggcttcgctc ttcttcgagt cggccaacca gtccctcgtc
     1561 gcgacgatcc gggacacgca gacggctccg agcgtcgccg gcacactgcg gaccaacatc
     1621 ggaattcgca atactgacat ccgaggaacc gcccagaccg tctcggtcca gttgtccttc
     1681 tacgacgtca ctccaggaaa cgcgaccaac ggacagcgag tcggaggcgt tctcacgctc
     1741 tcgaacctcc agccgggaga ggtccgtcag ctgaacaaca tccgcgcact ggccgcgatc
     1801 cccgacaatg tcgtcgggat gctcgcattc gcggacgtga tcggagcttc cgcgtccagc
     1861 cccacgatcg aagggtacgt gaacatcctg gacggaggca cgcaggacgg cgccttcttc
     1921 gagatgaagt gctccgatac cgacggctgc ggctagagat gagaggcgcg cgccgcgact
     1981 aggcccgttt cccgcgcaac cacgccgctc cgatgtcgcg gcggaagtgc tttccctcga
     2041 attcgacccg ctcggccgcc tcgtaggcgc gcccgagcgc ttcggacagc gacggcgccg
     2101 tcgcggtcac gacgatcacg cggcctcccc cggccatcag tcggtcgccc tcccgctccg
     2161 tgtgcgcgtg gaagagctgg acgcccggct gccgcgcggc ttcggtcaac cccgtgatcg
     2221 ccttgccgcc gacgctcacg cccgggtagc ccgccgaaca caggacgacg ccggcgcaga
     2281 cctcggcctt cacctcgaga ggtgaga
//
LOCUS       DASWAV010000312         2489 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U2665
            k141_497122, whole genome shotgun sequence.
ACCESSION   DASWAV010000312 DASWAV010000000
VERSION     DASWAV010000312.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960316
            Sequence Read Archive: SRR10260007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2489)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2489)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:47:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,929
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2489
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_497122"
                     /isolate="SMAG_U2665"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.36444 N 132.31889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1389
                     /locus_tag="VF958_06400"
     CDS             <1..1389
                     /locus_tag="VF958_06400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014398203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family peptidase"
                     /protein_id="HEX9148776.1"
                     /translation="EKVSVEKAGRTVDFKTMDDYVAFSGTQKPESRVEKAEIVFVGYG
                     IVAPEFQWDDYKDVDVKGKVLLMMNNDPEDDPKLFAGETRLWYGRWDYKYMMAGKKGA
                     VGAFIIHTQPSAGYPWQVVRTSWSGEHFELPYAGEPRVEIKGWFTEDASKKIASLGGQ
                     DLDRLREAAQKRDFRPVSLGVNVSMRIENSVQSKDSANVIGKLPGSDPKLASEAVVYT
                     AHHDHLGMKMDAKPGEDAIYNGALDNASGVAAMLGIAKAFEALPKAPRRTIYFASVTA
                     EEQGLLGSEYLAKHLPVPAGRIAANINIDGVSIFGRTRDITVVGYGKSSLDPIIVALA
                     KTEGRVVKPDQFPDRGFFYRSDQFNLAKEGVPAAYFDSGTDVIGKPAGWGKSRIEEWE
                     DKDYHQPSDELKSDWNLSGAVEDVRLDFWLGAKVANADRMPEWKKGDEFEAARKKALS
                     EAAVGGPGIGNR"
     gene            1424..1960
                     /locus_tag="VF958_06405"
     CDS             1424..1960
                     /locus_tag="VF958_06405"
                     /inference="COORDINATES: protein motif:HMM:NF024895.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HEX9148777.1"
                     /translation="MKRLLIPVAVGLFAASFARAQGTSGLHFGLEGGISNPQGDTKDV
                     YDNGWHGGALAVWNFPVVPVGLRVDGSYNKFDGRTATAGNAEVLTGTANVVLGFRALL
                     VKPYLLGGIGYYRLDFSDASFPSAFRGKSNETGWNAGAGVSISLRKVDLIVEARYHSV
                     STEGGRFKFVPVSVGLVF"
     gene            complement(1974..>2489)
                     /locus_tag="VF958_06410"
     CDS             complement(1974..>2489)
                     /locus_tag="VF958_06410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9148778.1"
                     /translation="HSLSYSRAPFKVVVNGSQVLNSNARDECFAHYAREQKELLDWIV
                     QQRIRGVVFLSGDRHETELIRVTPEGFYPLYDFTSSPLTAGLTNPMPKEEENNPLRVP
                     GTLVNDMHSFGLLRFSGACNERTLEMQVVDREGKRRWTQMVRGSDLVPPPPPGVTPGP
                     YRGHSSCAYLK"
BASE COUNT          463 a          768 c          847 g          411 t
ORIGIN      
        1 gagaaggtct cggtcgagaa ggcgggccgc acggtcgact tcaagacgat ggacgattac
       61 gtcgcgttct cgggaacgca gaagcctgag tcgcgcgtcg agaaggccga gatcgtcttc
      121 gtcgggtacg ggatcgttgc gcccgagttc cagtgggacg actacaagga cgtggacgtc
      181 aagggcaagg tcctcctgat gatgaacaac gacccggagg acgatccgaa actgttcgcg
      241 ggcgagacga gactctggta cggccgatgg gactacaagt acatgatggc ggggaaaaag
      301 ggcgccgtcg gcgcgttcat catccacacg cagccctccg ccggttatcc ctggcaggtc
      361 gtccgaactt cgtggtcggg ggaacacttc gagctgccct acgccggcga gccccgcgtc
      421 gagatcaagg ggtggttcac cgaggacgcg tcgaagaaga tcgcgtccct cggcggacag
      481 gacctcgacc gtctgcgaga ggcggcgcag aagagggact tccggccggt gtccctcggc
      541 gtgaacgtct cgatgcggat cgaaaactcg gtccagagca aggacagcgc gaacgtgatc
      601 ggaaagctgc cgggaagcga tccgaagctc gcgagcgagg cggtcgtcta taccgcgcac
      661 cacgaccacc tcgggatgaa gatggacgcg aagccaggag aggacgcgat ctacaacggc
      721 gcgctcgata acgcgtcggg agtcgcggcg atgctcggga tcgccaaggc cttcgaggcg
      781 ctgccgaagg cgccccgccg gaccatttac ttcgcttccg tcaccgccga agagcagggg
      841 ctcctcggat ccgagtacct cgcgaagcat ctgccggttc ccgccggccg gatcgccgcg
      901 aacatcaaca tcgacggggt cagcatcttc ggccgcacgc gggacatcac ggtggtcgga
      961 tacggcaagt cgagcctcga tccgatcatc gtcgcactcg cgaagacaga gggccgggtc
     1021 gtcaagccgg accagtttcc ggaccgcggc ttcttctacc gctccgacca gttcaacctg
     1081 gccaaggagg gggtgcctgc cgcgtacttc gacagcggca ccgacgtgat cggcaagccc
     1141 gccgggtggg ggaagtcgag gatcgaggag tgggaggaca aggactacca ccagccctcc
     1201 gacgagctga agagtgactg gaacctctcc ggcgcggtcg aagacgtgcg gctcgatttc
     1261 tggctcggcg cgaaggtcgc caacgcagat cgcatgccgg agtggaagaa gggcgacgag
     1321 ttcgaggcgg ctcgcaagaa ggcactgtcg gaggcggcag tcggaggacc gggaatcggt
     1381 aatcggtaat ccggaatcga aattgcaccg aagttctcac accatgaaga gactcctgat
     1441 tccggtggcg gtcggcctct tcgccgcgtc ctttgcccgg gcgcagggga cgagtggact
     1501 tcacttcggg ctcgaaggcg ggatctccaa cccccaggga gacaccaaag acgtctacga
     1561 caacgggtgg cacgggggag cgctcgccgt ctggaatttt ccggtcgttc cggtcggcct
     1621 tcgcgtcgac ggctcgtaca acaaattcga cggccggacg gcgacggccg gcaatgcgga
     1681 agttctcacg ggtacggcga acgtggtgct cgggttccgc gcgctgctgg tgaagccgta
     1741 cctcctcggc ggcatcgggt actaccggct cgacttctcc gatgcctcct tcccctcggc
     1801 gtttcgcggg aagtcgaacg agaccggctg gaacgcgggc gcgggcgtct cgatcagcct
     1861 gcgcaaggtc gatctgatcg tcgaggcgcg ataccacagc gtctcgaccg aggggggccg
     1921 tttcaaattc gttccagtat ccgtcgggct cgtcttctag ccgggcggca gctttatttc
     1981 aggtacgcgc aggacgagtg cccgcggtag ggccccggcg tcacgcccgg cggcggcggc
     2041 ggcacgagat cgcttccgcg caccatctgc gtccagcggc gctttccctc ccggtcgacg
     2101 acctgcatct ccagcgttcg ctcgttgcac gcgcccgaga accggagcaa cccgaagctg
     2161 tgcatgtcgt tgacgagcgt gcccggcacc cgcagcgggt tgttctcctc ctccttcggc
     2221 atcgggttcg tcagtcccgc cgtgagcggt gacgacgtga agtcgtagag cgggtagaag
     2281 ccctcgggcg tcacccggat gagctcggtc tcgtgccggt cgcccgagag gaaaacgacg
     2341 ccgcggatcc tctgctggac gatccagtcg aggagttcct tctgttcgcg agcgtagtgg
     2401 gcgaagcact cgtcccgcgc gttcgagttc aatacctggc tgccgttcac gacaaccttg
     2461 aagggcgccc ggctgtatga gagcgagtg
//
LOCUS       DASWAV010000313         2399 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U2665
            k141_55493, whole genome shotgun sequence.
ACCESSION   DASWAV010000313 DASWAV010000000
VERSION     DASWAV010000313.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960316
            Sequence Read Archive: SRR10260007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2399)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2399)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:47:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,929
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2399
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_55493"
                     /isolate="SMAG_U2665"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.36444 N 132.31889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            567..869
                     /locus_tag="VF958_06415"
     CDS             567..869
                     /locus_tag="VF958_06415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9148779.1"
                     /translation="MTCLAIDPADPETIYAGTARIGFFSPDGGVFRSLDGGQTWEPFG
                     SGLPPEGVTHIVIDPTGRTVHAGTSAGVYDYEIVPGARPPIVPGRSRTTRVLPSRP"
     gene            complement(986..2272)
                     /locus_tag="VF958_06420"
     CDS             complement(986..2272)
                     /locus_tag="VF958_06420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9148780.1"
                     /translation="MAATGGKPVQVTKFDASRRETTHRWPFFLPDGRRFLYFSGSHST
                     GAESELDAIFVGSLGGEKPKLLVNARSNAAYAAGHLLFVRQRTLLAQQFDPKTLKLTG
                     EAFPLAENVQDDPGFFNAVFSVSSEGTLAYQEAGGTTGLSELVWVDRSGRKIDVLGEP
                     GNYYEPRISPDGRRVAVGVGDPGDIWIYDVARRVRTRLTFAGGDDFASTWSPDGSRVA
                     FSSQRSGSGDLYAKDASGTGADELLASSNVFKVPNSWSPDGRYLAFVAFQGAPGSKAD
                     IWLLSLPDRKASPLLHGEFDEVAASFSPDGRWLAYTSNESGQPEVYVQPFPGPGGKWQ
                     VSTAGGQHTRWRRDGKELFYFAPDGKVMAVECHAEAAFEARTPQALFTTPFKHVPGPP
                     YDATADGQRFLLNRPIGEESSPPITLVQNWPALLKR"
BASE COUNT          420 a          755 c          792 g          432 t
ORIGIN      
        1 tggtattcgc gacgacgatt cgctcgcaat acctgggcga gggtggtgag gtgtatcgaa
       61 gtccggacgg aggttcgacc tggacccaac tcacctccgt gtccgcgagg gttcgatcgc
      121 tcgtcccaca tccaaccgaa cccgacaccc tcttcgccgc cagcaactgg tgtcgctgtc
      181 agcaagctct ctgcttcttc cactttgttt gcagcctgac gattctgcgg agccgcgatt
      241 ccggcgcaac gtggagtttt tacgacaccg gactccaggg aggctcgact gcgagcgtcg
      301 tcgtcgatcc agtcgaccac gaccgggttt tcgcgggagg cgacacggga attgcggtga
      361 gctcggatag aggcgatcac tggacagcga gcagcgcggg actcgaaaca tgcccgagta
      421 tcctggctct cgcggtccgc tcgaccgacg gcacgttgtt cgcggcttca ggccagattt
      481 cttcgaaccg gtttcagtgt gggggtgttt ttcgaagtct cgatgggggc cggacgtggt
      541 cggcgacgag cttatcgccg cattacgtga cctgcctcgc gattgacccg gcggaccccg
      601 agaccatcta tgcaggcacc gcaagaatcg ggtttttctc gcccgacggc ggagtcttcc
      661 gaagcctcga tggaggccaa acctgggagc ctttcgggag tgggttgccg ccggaaggcg
      721 tcacccacat tgtcatcgat ccgactggcc ggaccgttca cgccggcact tccgcgggcg
      781 tctacgacta cgagatcgtg ccgggcgcgc gaccgccgat cgtgcccggc cgaagtcgga
      841 cgacccgtgt ccttccgagc cgaccctagt cgggattcgt ggcggagagg gccgggttcg
      901 aaccggcggt tattagcccc acggcggttt aacaagccgc tggccaggct aaccgatccg
      961 ggcgattctc aaaggccagg cccgcctaac gcttcagaag cgccggccag ttctggacga
     1021 gcgtgatcgg aggcgaggac tcctcgccga tgggacggtt gagcaggaac cgttgaccat
     1081 cggcggtggc gtcgtaggga ggaccgggaa cgtgcttgaa gggggtggtg aacagagcct
     1141 gcggcgtcct ggcctcgaag gccgcttcgg cgtggcattc cacagccatg acctttccgt
     1201 cgggagcgaa gtagaacagc tcctttccat cgcggcgcca tctcgtgtgc tggccgcccg
     1261 ccgtcgagac ctgccacttc ccgcccggcc ccggaaaggg ctggacgtaa acctcgggtt
     1321 ggccggattc gtttgaggtg tacgcgagcc agcgcccgtc cggggaaaag gacgcggcca
     1381 cctcatcgaa ctcgccgtgg aggagcggcg aggccttgcg gtccggaaga gagagcagcc
     1441 agatatccgc cttcgagccg ggtgcaccct gaaaggcgac gaatgcgaga tatcgcccgt
     1501 cgggagacca gctgttcggg accttgaaga cattcgagct cgcgaggagt tcgtcggcgc
     1561 ccgtccccga tgcgtccttc gcgtacaggt ctccgctgcc cgaccgctgc gaggaaaagg
     1621 ccacgcgaga tccgtccggc gaccaggtgg aggcgaagtc gtcgccgccg gcgaacgtca
     1681 ggcgggttcg aacgcgccgc gcgacgtcgt agatccagat gtctcccgga tctccgacgc
     1741 cgaccgcaac gcggcggccg tcgggtgaga ttcgcggctc gtagtagttg ccgggctccc
     1801 ccagcacgtc gatcttcctt cccgatcggt cgacccagac gagctccgaa agcccggtcg
     1861 tgccgcccgc ctcctggtac gcgagagtcc cttctgagga gaccgagaac accgcgttga
     1921 agaagccggg gtcatcctga acgttttcgg cgagcggaaa cgcttcgccg gtgagcttca
     1981 gcgtctttgg gtcgaactgc tgcgcgagaa gcgtcctctg gcgaacgaag agcaggtggc
     2041 ccgccgcgta ggcggcattg gagcgtgcgt tgacgagaag cttcggcttc tctccgccga
     2101 gggacccgac gaagatcgcg tcgagctcac tttccgcgcc cgtcgagtgg cttcccgaaa
     2161 aatagagaaa gcgacgcccg tctggaagga aaaacggcca tcggtgtgtc gtctcgcgac
     2221 gggaagcgtc gaacttcgtg acctggaccg gtttccctcc ggttgcggcc actcggtgaa
     2281 gtgtttcccg gaactgcggt tcgaagagga tggtcccatt ccggctccag cttcctccac
     2341 gcgcgtccga ggcatcgcag atcgagagcg gaggaccgcc ggaaacctcg atcttcttc
//
LOCUS       DASWAV010000314         1893 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U2665
            k141_656153, whole genome shotgun sequence.
ACCESSION   DASWAV010000314 DASWAV010000000
VERSION     DASWAV010000314.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960316
            Sequence Read Archive: SRR10260007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 1893)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1893)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:47:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,929
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1893
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_656153"
                     /isolate="SMAG_U2665"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.36444 N 132.31889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1018)
                     /locus_tag="VF958_06425"
     CDS             complement(<1..1018)
                     /locus_tag="VF958_06425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020710547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0009410 - response to xenobiotic stimulus
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HEX9148781.1"
                     /translation="MLLAIPMVAEMVMESIFAIADIFWVSKLGADSVATVGITESMLA
                     LIYAVAIGVSIGATATVARRIGEKDPEHAATAAVQAIALGISLAIPIGLAGALLAPKL
                     LVLMGGSPALVATGWRYTAIMLGGNVIIMLLFLINAIFRGAGDAAIAMRVLWFANILN
                     IALGPCFIFGLGPFPKLGVTGAAVATTIGRGSGVLYQLWTLSRRRGRIVIERRHWKLE
                     PAAMLRMLRLSGNAIFQILIGTASWIGLVRIISAFGSAALAGYTIGIRIIVFAILPSW
                     GMSNAAATLVGQNLGARRPDRAERSVWIAGFYNMIFLGSVGLLFVAGAPILIRFFTTD
                     PSIAP"
     gene            complement(1102..>1893)
                     /locus_tag="VF958_06430"
     CDS             complement(1102..>1893)
                     /locus_tag="VF958_06430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020263542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent transferase"
                     /protein_id="HEX9148782.1"
                     /translation="YDFIDADDPGSWEAKVRPETKAVYVETLTNPLLELADLEAIAKF
                     ARARGLVSIIDNTFATPVNFRPLEHGFDLSVHSCTKYMNGHSDIVAGAVMGRREHIDR
                     IKHKLDHFGGSLDPHAAFLLHRGVKTLSVRVRWQNESALGIAKFLASRAEVARVNYPG
                     LPSHPRHERARRLFSGFGGMLSFELHGGAAAAKRFISATKLPIKAPSLGGAETLLTQP
                     AVTSHAGMTREDRQRLGITDGLVRMSVGLEATEDIVEDFAQALGK"
BASE COUNT          329 a          626 c          650 g          288 t
ORIGIN      
        1 acggcgcgat cgagggatcc gtagtgaaaa accggatgag gatcggtgcg ccggcgacga
       61 agagaagacc caccgagccg agaaagatca tgttgtagaa gccggcgatc cagaccgatc
      121 gctccgctcg gtccggcctc cgcgcgccga ggttctggcc cacgagcgtc gccgcggcat
      181 tcgacatccc ccacgagggc agtatcgcga aaacgatgat ccggatcccg atcgtgtagc
      241 cggcaagcgc tgcgctgccg aacgccgaaa tgatgcgcac gagcccgatc cagctcgccg
      301 tcccgatcag gatctggaag atggcgttgc cggagagccg cagcatccgc aacatcgccg
      361 ccggctcgag cttccagtgg cgccgctcga tcacgatgcg cccgcgtcgt cgcgacagcg
      421 tccagagctg atagagaacg ccgcttcccc ggccgatcgt cgtcgcgacg gccgctccgg
      481 tcacgccgag cttcggaaac ggtccgagcc cgaagatgaa acagggcccg agagcgatgt
      541 tcaggatgtt ggcgaaccac agcacgcgca tcgcgatcgc ggcgtcgccc gcgccgcgga
      601 agatcgcgtt gatgagaaac aggagcatga tgatgacgtt gccgccgagc atgatcgcgg
      661 tgtagcgcca tccggtcgcg acgagcgcgg gcgagccgcc catgagcacg agcagcttcg
      721 gcgcgagcag cgctccggca agcccgatcg gtatggcgag ggagattccg agggcgatcg
      781 cctggacggc ggcggtcgcc gcgtgctccg ggtccttttc gccgatccgc ctcgcgaccg
      841 tcgccgtggc gccgatcgag actccgatcg cgacggcgta gatgagcgcg agcatcgact
      901 cggtgattcc gacggtcgcg acggaatcgg ctccgagctt cgaaacccag aagatgtcgg
      961 cgatcgcgaa gatggactcc atgaccatct ccgcgaccat cgggatcgcg agcagcacga
     1021 ccgcccggcc gatcgagccc tcggtgtaat cgcggtgcga gccgcggacg gcctcgcgca
     1081 gcgtggtcca gacgcggatg gttacttccc cagggcctga gcgaagtcct cgacgatgtc
     1141 ctcggtcgcc tcgaggccga ccgacatccg cacgagcccg tcggtgatgc cgagtctctg
     1201 gcggtcctcg cgcgtcatcc ccgcgtgcga cgtgaccgcg ggctgcgtca gcagcgtctc
     1261 ggctccgccg aggctgggag ccttgatcgg gagcttcgtc gcggagatga agcgtttcgc
     1321 ggcggccgcg ccgccgtgga gctcgaatga aagcatcccg ccgaagccgg agaagaggcg
     1381 ccgcgcgcgc tcgtgccgcg ggtggctcgg aagtccgggg tagttgaccc gcgcgacctc
     1441 ggcgcgagag gcgaggaatt tcgcaattcc gagcgcgctc tcgttctgcc agcgcacccg
     1501 caccgacaac gtcttgacgc cgcggtgcag gagaaacgcg gcgtgcgggt cgagggagcc
     1561 cccgaagtgg tcgagcttgt gcttgatccg gtcgatgtgc tcgcggcgcc ccatcaccgc
     1621 gcccgccacg atgtcggagt ggccgttcat gtacttcgtg caactgtgca cggagagatc
     1681 gaagccgtgc tcgagcggcc ggaagttgac cggcgtcgcg aaggtgttgt cgatgatcga
     1741 gacgaggccc ctcgcgcgcg cgaacttcgc gatcgcctcg aggtccgcaa gctccaacag
     1801 cgggttcgtg agcgtctcga cgtagaccgc cttggtctcc ggccggacct tcgcctccca
     1861 gcttcccgga tcgtcggcgt cgatgaagtc gta
//
LOCUS       DASWAV010000315         1515 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U2665
            k141_537191, whole genome shotgun sequence.
ACCESSION   DASWAV010000315 DASWAV010000000
VERSION     DASWAV010000315.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960316
            Sequence Read Archive: SRR10260007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 1515)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1515)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:47:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,929
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1515
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_537191"
                     /isolate="SMAG_U2665"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.36444 N 132.31889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..327
                     /locus_tag="VF958_06435"
     CDS             <1..327
                     /locus_tag="VF958_06435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9148783.1"
                     /translation="WAHDYEKDGKTAAALSVLSSATRSNPASEEIARVLAVELFRAKD
                     CRRGLAALSRFEAATKSTDTLNVLALLQTCLENRPEVVRLLRRSLEIDPNQPEVAHSL
                     RAAEGS"
     gene            complement(379..>1515)
                     /locus_tag="VF958_06440"
     CDS             complement(379..>1515)
                     /locus_tag="VF958_06440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9148784.1"
                     /translation="NDGLKFTILRGDFTIPNANIPTCAAAPQGAPACTYLDTVTFNFE
                     DQIQPRLGLAFEADPHVHDKFYANYARYSNMDNQSFARSAAPVRIERHDAYFDRTTGA
                     LITEVVRDNQTGKRVLEHIDPTKTDEVLFGYARPFGSGWAAELWGMYRYTDDIIEDFP
                     GVNRTTASGFRYGNIPAHRKYRAATVEVRKAYSDRWTLDVSYTLSKLEGNWDLDGTGV
                     AVFYSSSYIEDGPGLYVEDPFRNGTLAGDRKHVAKAFASYELFTNTTIGGYLRFQSGR
                     PWEARKYDAVNGVALEYAEKAGSRRLGSWTNFDLQVTQRIPLNVVEIVAGVRIGNLFN
                     TQPVLAVDQVLYDGTSNSNPNPNFGKPTAYAAPRRFTLIASVNF"
BASE COUNT          254 a          468 c          504 g          289 t
ORIGIN      
        1 tgggcgcacg attacgagaa ggatggaaag acagctgcgg cgctgtcggt cctctcgagc
       61 gcgacccggt cgaacccggc gagcgaggag atcgcccgag tgctcgcggt ggaactcttc
      121 cgcgcgaagg actgccggcg cggtctcgca gccctctccc ggttcgaagc agcgacgaag
      181 agcacggaca ccttgaacgt cctcgccctt ctccagacgt gcctcgagaa ccgcccggag
      241 gtggttcgcc ttctgcggcg ctcgctcgag atcgacccga accagcccga ggtcgcgcat
      301 tcgctccgcg cggccgaagg ctcctgaaaa agaagagccg gggcgaaccc cggctcttcg
      361 gattacagat cgggctgact aaaagttgac gctggcgatg agcgtgaacc tcctcggcgc
      421 cgcgtaggcg gtcggcttcc cgaagttcgg attcggattg ctgttcgacg taccgtcgta
      481 gagcacctgg tcgaccgcca gcacgggttg cgtgttgaaa aggttgccga tgcgaacgcc
      541 ggcgacgatt tccaccacgt tgagcgggat ccgctgcgtg acctggagat cgaaattggt
      601 ccaggacccc agtcgccgac ttcccgcctt ctccgcgtac tcgagcgcca cgccgttgac
      661 cgcgtcgtac ttgcgcgctt cccacggccg gccgctctgg aacctcaaat agccgccgat
      721 cgtcgtgttc gtgaagagct cgtagctcgc gaaggctttc gcgacgtgtt tgcgatcgcc
      781 ggcgagtgtg ccattgcgga acgggtcttc gacgtaaagg ccgggaccgt cctcgatgta
      841 cgacgagctg tagaagaccg ccacgcccgt gccgtcgagg tcccagttcc cctcgagctt
      901 cgagagcgtg taggagacgt cgagcgtcca gcggtcgctg taggccttcc gcacctcgac
      961 cgtcgccgcc cggtacttgc ggtgcgccgg gatgttgccg tagcggaagc cggaagcagt
     1021 cgtccggttc acgcccggga agtcttcgat gatgtcgtcg gtgtagcggt acatccccca
     1081 gagctcggcc gcccatcccg agccgaaggg acgggcatac ccgaagagca cctcgtcggt
     1141 cttcgtcggg tcgatgtgct cgagcactct ttttccggtc tggttgtcgc ggacgacttc
     1201 ggtgatcagc gcgccggtgg tccgatcgaa ataggcgtcg tgccgctcga tgcggaccgg
     1261 tgccgccgag cgcgcgaacg actggttgtc catgttcgag tagcgggcgt agttcgcgta
     1321 gaacttgtcg tgaacgtgag gatcggcctc gaatgcgaga ccgaggcgcg gctggatctg
     1381 atcctcgaag ttgaacgtga ccgtgtcgag ataagtgcag gctggcgctc cctgcggggc
     1441 tgcggcgcag gtcgggatgt ttgcgttcgg tatcgtgaaa tctccacgca ggatcgtgaa
     1501 cttgagtccg tcgtt
//
LOCUS       DASWAV010000316         2727 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U2665
            k141_417574, whole genome shotgun sequence.
ACCESSION   DASWAV010000316 DASWAV010000000
VERSION     DASWAV010000316.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960316
            Sequence Read Archive: SRR10260007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2727)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2727)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:47:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,929
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2727
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_417574"
                     /isolate="SMAG_U2665"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.36444 N 132.31889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(139..420)
                     /locus_tag="VF958_06445"
     CDS             complement(139..420)
                     /locus_tag="VF958_06445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019431732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquitin-like small modifier protein 1"
                     /protein_id="HEX9148785.1"
                     /translation="MPVRFFVPGPLREFAAGRAEVTLAVTPRTLRDALAALARDYPGV
                     ARRVLDEQGQVRPHVNLFVGGEAVRFADGLDTPVADGSEIAILPAVSGG"
     gene            complement(420..1583)
                     /locus_tag="VF958_06450"
     CDS             complement(420..1583)
                     /locus_tag="VF958_06450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007453486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exo-alpha-sialidase"
                     /protein_id="HEX9148786.1"
                     /translation="MAAKKISVRNGDVLLLVGTMKGAFLLKSDRSRRRWERSGPHFPG
                     SSVYAIGYDGRNDRHRIWAAPKSLHWGAELCSSDDFGAKWSRPEVPRIAFPKETGATL
                     AHIWQIEPGLDDEPERIFVGVEPSALFESRDGGESWSLNEGLWNHPHRAKWTPGGGGL
                     CLHTILTDPARRDRLTIATSTGGVYRTDDNGETWRARNRGIQAYFLKEKDPEFGQCVH
                     KVVRHPSRPDRMFLQHHFGLYRSDDGGDTWREASKGVPSDFGFPMAMHPRDTDTVYIV
                     PLQADQFRCPPEGRLRVYRTRDGARSWKPLTRGLPQKDCYDTVLRDAMAVDEEDPAGV
                     YFGTRNGWLYGSRDEGQSWSAIEESLPPVTCVRAVTIRSVGKARPARRRRKAA"
     gene            complement(1639..2229)
                     /gene="coaE"
                     /locus_tag="VF958_06455"
     CDS             complement(1639..2229)
                     /gene="coaE"
                     /locus_tag="VF958_06455"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: protein motif:HMM:TIGR00152.1"
                     /note="Dephospho-CoA kinase (CoaE) performs the final step
                     in coenzyme A biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HEX9148787.1"
                     /translation="MIRVGLTGGLASGKSTVAARLEKLGAEVFDADQIVAELYVSGGP
                     GEAAAWDLFGDAVFDDHGAIDRGRLARLVFSDPEKRRALESRIHPLVGTEIARRFAEA
                     ERRGAAVAVAEASQLLEAKKEGPYDRVLLVVAPETERVRRWTAKGGDANDARRRMAAQ
                     IPPEEARRRATDVIVNDGTVEELERKVDELFGTWVE"
     gene            complement(2231..>2727)
                     /locus_tag="VF958_06460"
     CDS             complement(2231..>2727)
                     /locus_tag="VF958_06460"
                     /inference="COORDINATES: protein motif:HMM:NF014081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX9148788.1"
                     /translation="HGSFLDDEALRLMKERGTYFVPTLMAGEYAGGRKAKRQYPPEIA
                     AKAKAALEARSSAMRAAVKAGVKIAFGTDAGGFPWTEINQAQEFEHEVRLGMSPLEAI
                     RSATTVAAELLGTSGRAGAVEAGAWADLVAVTGDPLKDVSVLSRIDFVMKNGEVVRAP
                     APRE"
BASE COUNT          391 a          983 c          921 g          432 t
ORIGIN      
        1 tcggtttcgg cgtacgcagg gacttcgacg gcatgaccaa acgagccatc gaagcgctcg
       61 acgagcgcca cttcgggttc cgcgctctgt gagagatagc gggagatcgc ttcggacacg
      121 acggaggacg gtccccgctc agcccccgct gactgcgggc agaatcgcga tctcgctccc
      181 gtccgcgaca ggcgtgtcga ggccgtctgc gaatcggacg gcctcgccgc cgacgaagag
      241 attcacgtgt gggcgcacct ggccctgctc gtcgaggacg cgcctcgcca ccccggggta
      301 gtcgcgggcg agcgcggcga gcgcgtcccg gagcgtgcgg ggcgtcacgg cgagcgtgac
      361 ttccgcgcgg cctgcggcga actcgcgcag cgggcccggc acgaagaagc ggaccggcat
      421 cacgccgcct tccgccggcg gcgcgccggg cgcgccttgc cgacgctgcg aatcgtaacg
      481 gcgcggacgc acgtgaccgg cggcaggctt tcctcgatcg ccgaccagct ctggccctcg
      541 tcgcgcgacc cgtagagcca gccgtttcgc gtgccgaagt acacgccggc cgggtcctcc
      601 tcgtcgaccg ccatcgcgtc gcgcagcacg gtgtcgtaac agtccttctg aggaagtccc
      661 cgggtgagcg gcttccagga ccgggcgccg tcgcgcgtcc ggtagacgcg gagcctgccc
      721 tcgggagggc accggaactg gtccgcctgg aggggcacga tgtacaccgt gtcggtgtcg
      781 cggggatgca tcgccattgg aaagccgaaa tccgacggca cgcccttcga agcttcgcgc
      841 caggtgtcgc cgccatcgtc cgagcgatac agaccgaagt ggtgctgcag gaacatccgg
      901 tcgggtcgcg acggatggcg aacgaccttg tggacgcact gcccgaactc cgggtccttc
      961 tccttgagga agtacgcctg gatcccgcgg ttgcgggccc gccaggtctc cccgttgtcg
     1021 tcggtgcggt agacgccgcc ggtcgaggtc gcgatggtga gccggtcgcg gcgcgccgga
     1081 tccgtcagga tggtgtggag gcagagcccg ccgccgcccg gcgtccactt cgcccggtga
     1141 gggtggttcc agaggccttc gttcaacgac cagctctctc cgccgtcgcg ggattcgaac
     1201 agagcggagg gctcgacgcc gacgaagatc cgctcgggct cgtcgtcgag accgggctcg
     1261 atctgccaga tgtgcgcaag ggtggcgccc gtctccttcg ggaacgcgat gcgcgggacc
     1321 tccgggcggc tccacttcgc gccgaagtcg tcgctcgagc agagctcggc gccccagtgg
     1381 agactcttcg gcgccgccca gatccgatgg cgatcgttgc gcccgtcgta gccgatcgcg
     1441 tagaccgagc ttccgggaaa gtgcggtccg ctcctttccc accggcgacg agagcggtcg
     1501 gacttcagaa ggaaggcgcc tttcatcgtg ccgacgagca ggagaacgtc gccgttcctg
     1561 acggagattt ttttcgcggc catgacccct atcctaatcg ctcgtcgaga ccgctcgacg
     1621 cggccggagc acgcgcgact attcgaccca ggtcccgaac aattcatcga cctttcgttc
     1681 gagctcctcg acggtcccgt cgttcacgat gacgtcggtc gcgcgacgtc gcgcttcctc
     1741 cggcgggatt tgcgcggcca ttcgtcgccg cgcatcgttc gcgtcgccgc ccttcgcggt
     1801 ccagcgccgc acccgttccg tctccggcgc cacgacgagg agcacccggt cgtaaggccc
     1861 ctccttcttt gcctcgagga gctgcgatgc ctcggcgacc gcgacagcgg cgccgcggcg
     1921 ctccgcctcc gcaaagcggc gcgcgatctc cgtgcccacg agcggatgga tgcgcgattc
     1981 gagggcccgc cgcttctccg gatcggagaa gacgagccgg gcgagccggc cgcgatcgat
     2041 cgccccatgg tcgtcgaaca ccgcgtcccc gaagaggtcc cacgctgctg cctctccggg
     2101 gccaccggag acgtagagct ctgcgacgat ctgatccgcg tcgaagacct cggccccgag
     2161 cttctccagg cgcgccgcga ccgtggactt gcccgaagcg aggccgcccg tcaacccgac
     2221 ccggatcacc tcactcgcga ggcgccggcg cgcgcacgac ttcgccgttc ttcatgacga
     2281 agtcgatccg ggagaggacc gacacgtctt tcaggggatc gccggtgact gcgacgaggt
     2341 ccgcccacgc gcccgcctcg acggctccgg cgcggccgct cgtcccgagg agctccgcgg
     2401 cgaccgtcgt cgcgctccgg atcgcctcga ggggcgacat gccgagccgc acctcgtgct
     2461 cgaactcctg cgcctggttg atctcggtcc acgggaatcc gccggcgtcg gtgccgaacg
     2521 cgatcttcac cccggccttc accgccgccc tcatcgcgct ggaacgcgcc tccagggcgg
     2581 ccttcgcctt ggccgcgatc tcgggcgggt actgccgctt ggccttgcgc ccgcctgcgt
     2641 actctccggc catcagggtc gggacgaagt aggtgccgcg ttccttcatc aggcgcaagg
     2701 cctcgtcgtc gaggaacgag ccgtgct
//
LOCUS       DASWAV010000317         2893 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U2665
            k141_574555, whole genome shotgun sequence.
ACCESSION   DASWAV010000317 DASWAV010000000
VERSION     DASWAV010000317.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960316
            Sequence Read Archive: SRR10260007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2893)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2893)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:47:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,929
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2893
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_574555"
                     /isolate="SMAG_U2665"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.36444 N 132.31889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..262)
                     /locus_tag="VF958_06465"
     CDS             complement(<1..262)
                     /locus_tag="VF958_06465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008655392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRTAC1 family protein"
                     /protein_id="HEX9148789.1"
                     /translation="MRRTRSATALVLWLSTLVALGGCRQEKKTAPAALPTPVPAPAVR
                     FEEHAADLGISFVHVNGAQGKKWMPETMGGGVAVLDYDGDGRP"
     gene            complement(274..>2893)
                     /locus_tag="VF958_06470"
     CDS             complement(274..>2893)
                     /locus_tag="VF958_06470"
                     /inference="COORDINATES: protein motif:HMM:NF024827.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX9148790.1"
                     /translation="YAMLLTHLALGVAIAIPFFVFAITHAKRMIRMWNKRAKIAGLAI
                     FTLAILCVSTGLFLTFRGATIPNRFVWIAHVSAVPLALVAFILHRRAHTHQLQFKRLY
                     AWGAAVAIFLGAMALAAKLEKPPRRIVNTSGDTVFFPSSSETFDQGLLNGYKLAANEY
                     CKECHPDSFHQWERSAHRFSSFNNPFYRKSVELMADQVGRERTKWCSGCHDPVVLFTG
                     QMGAATQAKFSYDSWEAQQGLTCMSCHSIAEIKDVRGNGSYVIEESTQYPFAFSKNET
                     LRAINRLLIRMEPSLHRKTFLKPFHRTPEFCSSCHKVALIPALNDYHWMRGQNHYDSW
                     YDSGVSGRAVRSFYDPPKAKACRDCHLPPLQSAEFGNRGGFVHDHLFPAANTALPFIR
                     KDGETVARIQEFLKNKVLTVDLFAIRRGEDVLVLGEQPPEVAPGEVLDVEVVVRTRGV
                     GHPYTNGTADSNETWVSLEGASGKRPFFESGVLDAAGRLDPAADRLSVFLVDHEGGYM
                     DRRQPQDIHTTLYNNQIGPGAARVVHYRVRVPEEARESIELSAAVHYRKFSRDYTSFS
                     LGASSPSLPVTTLASDTVVLPINGASRESGTGNREATRGNPDKPWLRWNDYGIGLVLQ
                     GDLKGAARAWTRVAELAPDKPDGSLNRARAEIAEGRLADASASLALAEKIRPGWEKTA
                     FFRSIVARDEGRLGDAERDLRTVLAKFPLDRVAWNNLGGVLWLGGRFQESAEAYGKTL
                     AIDPEDLTAHYNLMRVYRAIGNRRLAAVHAAAYRKYKDDETGRAVAADLRRQDVWANR
                     ESLPIHVHEEASPPPAAPPGWVASLGPKGYQTDLGYLTRAHAPIRREAVEPAGAPLRK
                     STSYAPVSAPPHPGSK"
BASE COUNT          475 a          938 c          986 g          494 t
ORIGIN      
        1 cgggccgccc gtcgccgtcg tagtcgagga ccgcaacgcc gccgcccatc gtttccggca
       61 tccacttctt cccctgagcc ccgttgacgt ggacgaacga gatgccgagg tcggcggcgt
      121 gctcttcgaa tcgaacggcc ggcgccggca ccggggtcgg aagcgctgcc ggcgcggtct
      181 tcttctcctg ccgacagccg cccaacgcga cgagtgtcga aagccagaga accagagcag
      241 tcgcgctgcg cgttcttcgc aaaccggttg aacctacttc gagcccggat gcggcggcgc
      301 gctcaccggc gcgtaggacg tgctctttcg aagaggcgcg cccgccggct cgacggcttc
      361 ccgacgaatc ggcgcgtgcg cgcgcgtgag atacccgagg tccgtctgat accccttcgg
      421 accgagcgac gcgacccagc ccggaggtgc ggccggggga gggctcgctt cctcgtgcac
      481 gtggatgggg agtgattcgc ggttcgccca aacgtcctgc cggcgcaggt ccgccgccac
      541 ggcgcggccc gtttcgtcgt ccttgtactt ccggtaggcg gccgcgtgca cggccgcgag
      601 ccttcggttc ccgattgcgc ggtaaacgcg catcaggttg tagtgggcgg tcaggtcctc
      661 cgggtcgatc gcgagagttt tcccgtacgc ttcagcagac tcctggaaac gaccaccgag
      721 ccagagaacg ccgcccaggt tgttccaggc gacgcgatcg agcggaaact tcgcgaggac
      781 cgttcggagg tcgcgctcgg cgtcccccaa ccgaccctcg tcgcgggcaa cgatggagcg
      841 gaagaacgcc gtcttctccc agccggggcg gatcttttcg gcgagcgcga gagaggcgga
      901 ggcgtcggcg agccgcccct ccgcgatctc ggcccgcgcc cggttgagcg acccgtccgg
      961 cttgtccgga gcgagctcgg cgacgcgcgt ccacgcccga gccgcgccct tcaggtcgcc
     1021 ctggaggacg agcccgatcc cgtagtcgtt ccagcgcagc cagggcttgt cgggattccc
     1081 tcgggtcgct tcccgattcc cggttcccga ttcccggctc gctccgttga tgggcagaac
     1141 aacggtatcg ctcgccagcg tcgtgaccgg cagggacggg ctcgacgctc cgagcgaaaa
     1201 ggacgtgtag tcgcggctga acttccgata gtggacggct gcggacagtt cgatcgactc
     1261 ccgcgcttcc tccggaaccc gcacgcggta gtgaacgacg cgcgcggctc ccgggccgat
     1321 ctggttgttg tagagcgtcg tatggatgtc ctgcggctgc cggcgatcca tgtatccgcc
     1381 ctcgtgatcc acgaggaaga ccgagagccg gtcggcggcc ggatcgaggc ggcccgcggc
     1441 gtcgagcacg ccgctctcga agaatggtcg tttcccgctc gcgccctcga gtgacaccca
     1501 ggtctcgttg gagtcggccg ttccgttggt atacgggtga cccactccgc gtgtccgcac
     1561 gacgacctcg acgtcgagaa cctctccggg cgcgacctcc ggtggttgtt cgccgagcac
     1621 gagaacgtct tctccgcgcc ggatcgcgaa gagatcgacg gtcagcacct tgttcttcag
     1681 gaactcctgg attcgggcga cggtctctcc gtcctttcgg atgaagggca gggcggtgtt
     1741 cgcggccggg aagaggtgat cgtgcacgaa gccgccccga ttcccgaact ccgcggactg
     1801 gagcggcggc aagtggcaat cgcggcacgc cttcgccttc ggcggatcgt agaaggaccg
     1861 cacggcccgc cctgagaccc ccgagtcgta ccagctgtcg tagtggttct gtccgcgcat
     1921 ccagtggtag tcgttcagcg cggggatcaa ggcgaccttg tgacacgacg agcagaactc
     1981 cggcgtccgg tggaagggct tcaggaacgt cttgcggtgg agcgacggtt ccatgcggat
     2041 gagtaaccga ttgatcgccc ggagcgtctc gttcttcgaa aacgcgaagg ggtactgcgt
     2101 ggactcctcg atcacgtagg agccgttgcc ccggacgtcc ttgatctcgg cgatcgagtg
     2161 gcaggacatg caggtcagac cctgctgcgc ctcccaggag tcgtacgaga acttcgcctg
     2221 ggtggccgcg cccatctggc ccgtgaagag cacgacggga tcgtggcatc cggagcacca
     2281 tttcgtccgc tcgcggccga cctggtccgc catgagctcc acgctcttgc ggtagaacgg
     2341 gttgttgaac gacgagaacc ggtgcgccga gcgctcccac tggtggaacg agtcgggatg
     2401 gcactccttg cagtattcgt tcgcagcgag cttgtaaccg ttcaagagac cctggtcgaa
     2461 agtctcggag gaggacggga agaacaccgt gtcgccgctc gtgttgacga tgcgcctcgg
     2521 cggcttctcc agcttggcgg cgagcgccat cgccccgaga aagatcgcga ctgcggcccc
     2581 ccacgcgtaa agcctcttga actggagctg gtgggtatgc gcgcggcggt ggaggatgaa
     2641 cgcgacgagc gcgagcggaa ctgccgaaac gtgggcgatc cagacgaatc ggttggggat
     2701 cgtggcgcca cgaaacgtga gaaaaagccc ggtcgagacg cacaggatcg cgagggtgaa
     2761 gatcgcgagg cccgcgatct tcgcgcgctt gttccacatc cggatcatcc gtttcgcgtg
     2821 ggtgatcgcg aagacgaaga acggaatcgc gatcgcgacg ccgagcgcga ggtgcgtcag
     2881 gagcattgcg tag
//
LOCUS       DASWAV010000318         5096 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U2665
            k141_335833, whole genome shotgun sequence.
ACCESSION   DASWAV010000318 DASWAV010000000
VERSION     DASWAV010000318.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960316
            Sequence Read Archive: SRR10260007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 5096)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5096)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:47:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,929
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5096
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_335833"
                     /isolate="SMAG_U2665"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.36444 N 132.31889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..762
                     /locus_tag="VF958_06475"
     CDS             <1..762
                     /locus_tag="VF958_06475"
                     /inference="COORDINATES: protein motif:HMM:NF024829.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HEX9148791.1"
                     /translation="GANLAASRDSLSKTTVSAPIAGVVTYLPVKEGEVTVIGTMNNPG
                     TQLLTISDMSEVEAVMMVDETSVPEVKVGQKANLNIDAYPNRPFEGLVTEVGSSPIAK
                     NDPDLLSLTQGSEAINFKVKIRVVNPPETIRPGFSVTADILTGRKEGATAIPIQALVV
                     RDVPKKDGKPAGGGTGRPQTEEGVYVIKTGKLAFKKVETGLAGELMIEVKSGPKVGEE
                     IVTGPFKVLRQVKEGDKVIIEKEGGKGRQKTPAAA"
     gene            759..1973
                     /locus_tag="VF958_06480"
     CDS             759..1973
                     /locus_tag="VF958_06480"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX9148792.1"
                     /translation="MRSAELLRVSASALTRHKMRAFLTLLGVIIGVATVVSVVSVLSG
                     LNAYVKDKIIGLNPDVVIFTKYGIITSREEWLLARKRRDITLTDMAIIQRECKLCATV
                     GGRGDRRRPVKYMDQKLADVEIQGHTSNMGEAMSFDIVTGRYFTEAEYEHAAAVGVIG
                     WDVQDQLFPHMDPIGRVMKIDGFPVKVIGTLAKQGNVMGQSQDNVVYLPLTLFKKHIA
                     PSEDIGVFIKPLGGVAMVGQTTDEVRSILRSLRKTKFDKDDPFGLVTAEMLQDLWKSI
                     SAGAFLLMILISGISLVVGAIVIANIMFVSVVERTKEIGVRRALGARRRDIRWQFLLE
                     AALLSAVGGVVGVILGSLIAMAVNRVFPAQVKVSFIIVGIGVATVTGLIAGWIPSSQA
                     SKLAPVEALRYE"
     gene            2013..3287
                     /locus_tag="VF958_06485"
     CDS             2013..3287
                     /locus_tag="VF958_06485"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX9148793.1"
                     /translation="MSGILSHLRRGFGENVRFAFLAVRTHKLRAFLTVLGIVVGVATV
                     IAMVSIVTGFNNNMVRNFQSFGATLVQFQKYESRFGPGHRDESERRRKDLTYEDAVAL
                     KESIPEMRAVSPERYLWNADISVKYRDNETTTPVVAGVAPDYSVANNHFVAYGRFITE
                     SDFRHTASVAVIGEDVKKILFPREDPLEKVVNFNGEDFMIVGVLEKKGNAFGGSNDNF
                     FLIPFPTFDRKFPQIKNSGGDTIHIATVPYTPDQVPVIIEKGTALLRVRRHVPFNKPN
                     DFAIFTPDKLIESFQGITRGVTLAMVFIAMISLLIGGVGVMNIMLVSVTERTREIGVR
                     KAVGAYRRDIVMQFLTEATTLSLLGGAIGVVLGIAVPAGIKRAFAALPAETPAWAILV
                     GLGVSISVGIFFGLYPAVKASRLDPIEALRYE"
     gene            3327..4610
                     /locus_tag="VF958_06490"
     CDS             3327..4610
                     /locus_tag="VF958_06490"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX9148794.1"
                     /translation="MRGLFGFVRRGFGENVRFALLAVRTHKLRASLTVLGIVVGVATV
                     IAMVSIVTGFNNNMVRNFQSFGASLVLFQKYEPRFGPGGPTPEGELRRKDLTLEDAAA
                     LREIPEMRAVSPQRYLWNNTDYHLKHRDAEAQSPRVFGVMEEYPAATNRVVGLGRFMT
                     STEIDHAADVMVIGEEIREKLFPREEPIGKRVTLGHDGYTVVGVFEKKGKMFGESQDN
                     FVVIPITTFDRRFPWIKVGGGEGDALRIATVPYTPEQVPVIIEKARAVLRTRRHVRFD
                     KPDDFGIVTPDKMIESFQGITRGITLAMVFIAMISLLIGGVGVMNIMLVSVTERTREI
                     GVRKAVGAFRRDIVLQFLTEATTLSLLGGAIGVVVGIAVPAGVKKLFEALPAETPMWA
                     IVIGLAVSISVGIFFGLYPAVKASRLDPIEALRYE"
     gene            complement(4736..>5096)
                     /locus_tag="VF958_06495"
     CDS             complement(4736..>5096)
                     /locus_tag="VF958_06495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007923658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9148795.1"
                     /translation="GPEGMGARGLACATCHAAENPPASFGAHMPPGAPNWQLPPPEHK
                     MVFIGLSAGELCRRLKDKSENGGRDTVLLRKHLDDELVLWGWNPGVGRAPVSVPRAEF
                     VAAFERWAQAGAPCPAP"
BASE COUNT          933 a         1624 c         1661 g          878 t
ORIGIN      
        1 ggcgcgaacc tcgctgcgag tcgcgactcc ctctcgaaaa cgaccgtgag cgcgccgatc
       61 gccggcgtcg tcacctacct tccggtcaag gagggcgagg tcacggtcat cgggacgatg
      121 aacaatccgg ggacgcagct gctgaccatc tccgacatgt cggaagtgga ggcggtgatg
      181 atggtcgatg agacctccgt gcccgaagtg aaagtcgggc agaaggcaaa cctgaacatc
      241 gacgcgtacc ccaaccgccc cttcgaggga ctcgtcaccg aggtcggctc ctcgccgatc
      301 gcaaagaacg atccggacct cctttcgctg acgcaaggct ccgaggcgat caacttcaag
      361 gtcaagatcc gcgtcgtcaa tccgcccgag acgatccgac cgggcttctc cgtgacggcg
      421 gacatcctca ccggccggaa ggaaggcgcg accgcgatcc cgatccaggc gctcgtcgtc
      481 cgcgacgtcc cgaagaagga cgggaagccc gccggcggag ggaccgggcg gccacagacg
      541 gaagagggag tctacgtgat caagaccggc aagctcgcct tcaagaaggt cgagaccggg
      601 ttggcgggcg agctcatgat cgaggtcaag agcggaccca aagtcgggga agaaatcgtg
      661 accgggccgt tcaaggtcct tcgacaggtc aaggagggcg acaaggtcat catcgaaaaa
      721 gagggcggca aggggcggca gaagacaccg gcggcggcat gaggtcggcg gagctcctcc
      781 gcgtctcggc gtcggccctg acgcggcaca agatgcgcgc gttcctgacg ctgctgggcg
      841 tcatcatcgg cgtcgcgacg gtcgtgagcg tcgtctcggt gctctcgggg ctgaacgcct
      901 acgtcaagga caagatcatc gggttgaacc ccgacgtcgt catcttcacg aagtacggga
      961 tcatcaccag ccgcgaggag tggctgctcg cccggaagcg ccgcgacatc accctgaccg
     1021 acatggcgat catccagcgc gagtgcaaac tctgcgcgac cgtcggaggc cgcggcgacc
     1081 gccggcgccc cgtgaaatac atggaccaga aactcgccga cgtcgagatc caggggcaca
     1141 cttcgaacat gggcgaggcg atgagcttcg acatcgtcac cggccgctac ttcacggaag
     1201 cggagtatga gcacgccgcg gcagtggggg tgatcggctg ggacgtgcag gaccagctct
     1261 tcccgcacat ggacccgatc ggccgcgtga tgaagatcga cggtttcccg gtcaaggtca
     1321 tcggcacgct cgccaagcag ggaaacgtga tggggcagag ccaggacaac gtcgtctacc
     1381 tgccgctgac gctgttcaag aagcacatcg cgccgtccga ggacatcggc gtcttcatca
     1441 agcctctggg cggcgtcgcg atggtcggcc agacgaccga tgaggtccgc tcgatcctgc
     1501 ggtcgctccg caagacgaaa ttcgacaagg acgacccgtt cggcctcgtg accgcagaga
     1561 tgctccagga cctctggaag tccatctcgg caggcgcctt cctcctgatg atcctgatct
     1621 ccgggatctc gctcgtcgtc ggcgcgatcg tgatcgccaa catcatgttc gtgtccgtcg
     1681 tcgagaggac caaggagatc ggcgtccgcc gcgctctcgg ggcacgcagg cgcgacattc
     1741 ggtggcagtt ccttctcgag gcggcgcttc tctcggcggt cggcggagtc gtcggcgtga
     1801 tcctcggttc gctgatcgcg atggcggtca accgcgtctt cccggcgcag gtcaaggtgt
     1861 cattcatcat cgtcggaatc ggggtggcaa cggtcaccgg tctgatcgcg ggatggatcc
     1921 cctcgtcgca ggcgagcaaa ctcgcgcccg tcgaggcgct gcgctacgag tagtgagtcc
     1981 gaagtccgaa gtccgaagcc cgaagtagcg cgatgtccgg catcctcagt cacctccgcc
     2041 gcgggttcgg cgagaacgtg cgctttgcgt tcctggccgt tcgcacccac aagctccgcg
     2101 cgtttctcac ggtcctcggc atcgtcgtcg gcgtggcgac ggtgattgcg atggtttcga
     2161 tcgtcacggg gttcaacaac aacatggtcc gcaattttca gagctttggc gcgacgctcg
     2221 tgcagttcca gaaatacgag tcgcggttcg gcccgggtca ccgcgacgag tcggagcgcc
     2281 gccgcaagga cctgacctac gaggacgccg tcgcgctcaa ggagtcgatc ccggagatgc
     2341 gcgcggtctc gcccgagcgc tacctgtgga atgcggacat cagcgtgaag tatcgcgaca
     2401 acgagacgac gacgccggtc gtcgcgggcg tggcccccga ttattcggtc gccaacaacc
     2461 acttcgtcgc ctacggccgg ttcatcaccg agagcgactt ccggcacact gcgagcgtcg
     2521 ccgtcatcgg cgaagacgtg aagaagattt tgtttccacg cgaggaccct ctcgagaagg
     2581 tcgtgaactt caacggcgag gatttcatga tcgtcggcgt gctcgagaag aagggcaacg
     2641 cgttcggcgg atcgaacgac aacttcttcc tgattccgtt cccgacgttc gaccgcaagt
     2701 tcccgcagat caagaacagc ggcggcgaca cgatccacat cgcgaccgtt ccctacacgc
     2761 cggaccaggt cccggtcatt atcgagaagg gcacggcgct cctgcgcgtg cggcggcacg
     2821 tgccgttcaa caagcccaac gacttcgcga tcttcacgcc cgacaagttg atcgagagct
     2881 tccagggaat cacccgcgga gtgacgctcg cgatggtgtt catcgcgatg atctcgctcc
     2941 tgatcggcgg cgtgggcgtc atgaacatca tgctcgtgtc agtgaccgag agaacgcggg
     3001 agatcggcgt gcgaaaagcg gtcggcgcct accggcgcga catcgtgatg cagttcctga
     3061 ccgaggcgac gaccttgtcg ctcctcggag gagcgattgg agtcgttctc ggaatcgccg
     3121 tgcccgccgg gatcaagcga gcattcgccg cccttcccgc ggaaacgccg gcctgggcga
     3181 ttctcgtggg actcggtgtc tcgatttccg tcgggatctt cttcgggctc tacccggccg
     3241 tgaaggcgtc tcggttggat ccgatcgagg cgctgagata cgagtgagtt cgaagttaga
     3301 actccaaagc ccgaagtagc tcgagcgtga ggggactctt cggcttcgtc cgccggggtt
     3361 tcggcgaaaa cgtgcgcttt gcgctgctcg ccgtccgcac gcacaagctg cgagcttcgc
     3421 tgaccgtcct cggaatcgtg gtcggcgtcg cgacggtcat cgcgatggtc tcaatcgtga
     3481 ccggcttcaa caacaacatg gtgcgcaatt tccagagctt cggggcgtcg ctcgttcttt
     3541 tccagaagta cgaaccgcgc ttcggccccg gcggcccgac gccggaaggg gagcttcgcc
     3601 gcaaggacct gacgctcgag gacgccgcgg ctctgcgaga gatccccgag atgcgcgcgg
     3661 tctcgccgca gcgctatctc tggaacaaca ccgattatca cctgaagcac cgcgatgccg
     3721 aggcgcagtc tccccgcgtc ttcggagtga tggaggaata cccggcggcg accaaccggg
     3781 tcgtcgggct cgggcgtttc atgacctcga ccgagatcga ccacgccgcc gacgtcatgg
     3841 ttatcggcga ggagatccgc gagaagcttt tcccgcgcga ggagccgatc ggcaagcgcg
     3901 tcactctcgg ccacgacggt tacacggtcg tcggcgtatt cgagaagaag gggaagatgt
     3961 tcggggagtc gcaggacaac ttcgtcgtca ttccgatcac gacgttcgac cggcggttcc
     4021 cgtggatcaa ggtcggtggc ggagaagggg atgcgctgcg catcgcgacc gtcccttaca
     4081 cgcccgagca ggtccccgtc atcatcgaga aagcacgcgc cgttctccgg acgcgccggc
     4141 acgtccgatt cgacaagccg gacgatttcg gcatcgtgac gcccgacaag atgatcgaga
     4201 gcttccaggg catcacgcgc ggcataacgc tggcgatggt cttcatcgcg atgatctcgc
     4261 tcctgatcgg cggagtcggc gtgatgaaca tcatgctcgt gtcggtgacg gagaggacgc
     4321 gcgagatcgg cgtccgcaaa gcggtcggcg cttttcgccg cgacatcgtg ctgcaattcc
     4381 tgaccgaggc gaccactctc tctctgctcg ggggtgcgat cggcgtcgtc gtcggaatcg
     4441 ccgtgcccgc gggagtcaag aagctgttcg aggcgcttcc cgccgagacg ccgatgtggg
     4501 ccatcgtcat cggcctcgcg gtctcgattt ccgtcgggat cttttttggg ttgtaccctg
     4561 cggtcaaagc gtcgcggctc gacccgatcg aggcactgag gtacgagtag cgcccctccc
     4621 cgacagaatc acctcgccgc gtcgaggggg cgatcggcgt cgaatttctc tgcaaggaga
     4681 tcccccatca cggtttccgc tcagcgatcg ctcgcgcctc gcggaatgga aatcctcagg
     4741 gggccgggca gggcgccccg gcttgtgccc accgctcgaa agcggccacg aactcggcgc
     4801 gcggtacgct gaccggcgcg cgaccgacgc cgggattcca gccccacagc acgagctcgt
     4861 cgtccaggtg cttgcgcagg agtaccgtgt cgcggccgcc gttttccgac ttgtccttca
     4921 ggcgacggca cagctcgccg gccgaaaggc cgatgaagac catcttgtgc tccggcggcg
     4981 gaagctgcca gttgggggcg ccaggcggca tgtgcgcgcc gaagctcgcc ggcggattct
     5041 ccgcagcgtg gcaggtcgcg caggcgaggc cgcgagcccc catcccctcc ggtccc
//
LOCUS       DASWAV010000319        14677 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U2665
            k141_375005, whole genome shotgun sequence.
ACCESSION   DASWAV010000319 DASWAV010000000
VERSION     DASWAV010000319.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960316
            Sequence Read Archive: SRR10260007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 14677)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14677)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:47:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,929
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14677
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_375005"
                     /isolate="SMAG_U2665"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.36444 N 132.31889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(164..1414)
                     /locus_tag="VF958_06500"
     CDS             complement(164..1414)
                     /locus_tag="VF958_06500"
                     /inference="COORDINATES: protein motif:HMM:NF019040.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1501 domain-containing protein"
                     /protein_id="HEX9148796.1"
                     /translation="MTISRRDFLLRTSGFVTVSAMVPRWAVEGARRFEESVGADFVNR
                     TLVVLELAGGNDDLNTVVPYTDNLYLQLRSRIGVAAGTVLPLDGRLGLNPVMTGMKSL
                     WDANRLAVVENVGYPNSSLSHFAARDVWHTADPQLAQRHGWLGRWADRYVAGSSNPLS
                     CAAISQSLPRTLLADNVGVPSFVNLASYQYATDGQYAGDRNNQIRTFLTQNGGEYEVP
                     NTADALSRVGEDAISSSNILQTVGTGYVAGATYPAGTLGAGLLLIAQIVNANVGARIL
                     YITFGGFDNHATQTTDQPPLLTTFSNGIKALFDDLDAHGKSHDVLLMTWSEFGRRPQD
                     NGSNGTDHGTAGAHFVVGNAVLPGIYGNPPDLSHLDSNGNRAIQNDFRSYYGTILQDW
                     LGADAAAILGAGYPDLGFVNRAYA"
     gene            complement(1428..2801)
                     /locus_tag="VF958_06505"
     CDS             complement(1428..2801)
                     /locus_tag="VF958_06505"
                     /inference="COORDINATES: protein motif:HMM:NF020390.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1800 domain-containing protein"
                     /protein_id="HEX9148797.1"
                     /translation="MTVDWTREAAAHLSRRAGFGGTPAEIDDLYARGLEGAVSWLVDY
                     DSVSLTAYEASLAAKNYNVTTIRGVQQWFLDRMAFSPRPLEEKMNYFWNLHWTSGFAK
                     VQGVTLILNQNKTERQYAVARFDDLVLKISQDPAMLVWLDNWLNRAGRPNENYGRELM
                     ELFTLGIGNYSETDVREVARALTGWTLQNYRGSDGYNGATFLENPSLHDTGSKTILGQ
                     TGAWDGADAIRIILDHSDSEGSVSGRFIGAKLWTFFAYEAPPRHVVDELRDVYVSSNH
                     SIREVVRRILLHPEFYEAHTRKTWVRSPVEYAVAAIRLTEATSDFDVAVRQLAAMGQI
                     LFNPEDAKGWEWGESWMNTGTLFARATTVNTICTNRGTTGTRFDPARLLAGRDASTAE
                     KVVDILADRFNVADVSARVRASWIEYMNANDDGSRGTWTNTSANVDKKVRGLIHLMLT
                     GPAFHLA"
     gene            complement(2872..3999)
                     /gene="amrS"
                     /locus_tag="VF958_06510"
     CDS             complement(2872..3999)
                     /gene="amrS"
                     /locus_tag="VF958_06510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system radical SAM enzyme"
                     /protein_id="HEX9148798.1"
                     /translation="MASRAPLPKSPVAEVLSRRTIAAAPELVERLPDGRLKCFSCGHR
                     CPIPEGREGVCRVRYNEKGVLKVPWGYVGAIQCDPIEKKPFFHALPGSDALSFGMLGC
                     DLHCGYCQNWFTSQSIRDPQAVAPPRDVSPEGLAELAVECGAPTVVSTYNEPLITSEW
                     AVAVFREAKQRGLSTGYVSNGNGTPQVLDYIRPWIDFYKVDLKSMDDKHYRELGGVLQ
                     NVLDTIQGIHDRGIWLEVLTLVIPGFNDSEEELTRAAAFVASVSPDIPWHVTAFHPDY
                     KMTDRGGTPVSTLVRAAEIGTEQGLRYVYAGNLPGRVGKWEDTRCPGCATTLIERYGF
                     LVRKNRLADGACFRCQTPIPGRWDPRMEGTTKTHGIPLPVF"
     gene            4149..6665
                     /locus_tag="VF958_06515"
     CDS             4149..6665
                     /locus_tag="VF958_06515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9148799.1"
                     /translation="MPKSLRLSGLVAAVSLLSPLLRAQTDNPTTIPTGNAYETEKSGV
                     VSGVRIDVRPDGSVWFLVPALDRIAVLRGGETKQWALRDDNHLGANPVDFEIDGDSVW
                     FICNGESGVDAGHSIFGRLDTVTGQVREWEVPGSRPAGFYRALDGKVWIPQTDRRLQS
                     VDLTTLEVVDYRSPQTIAYSDIAVGPDGAFWMTDFGNNRIVRFEPGASEETSWTTFDP
                     ALAILNPSQIQFDAQGRLWISEFTGARMDRFDPATGDLVSFVGFLNPIHFDLFGGRVY
                     VTEAHGGNGQIALLDPSFATSGTTTLTSQTFDVGSLVNVRQATIHDHTVTPTTFASPA
                     TAIPAADLKVAAGAPGVLRTEIPWQNSYGIDVVRGEVWVGTAGRLAHVELQTVGTPSD
                     LVAPVATQLAGDATHESRVDLTFHNASDETISGELLYLYSPGFFAARQTFSLGPGATS
                     VVSDAFGDLGSSTIPVTGPIRVHVTSGPADALEVSVRTAQAREDGGSFGYSTPAFSPA
                     ESLGAGASRTLFTGFRESETSIFGFYSPSGAEAVFTLVAPDGTVRGELPISIISNIDE
                     EFSPAASAFGVPPEPGDVIRVSVASGTLLPWVRIVDGGSKDTALSLPARELASATFPN
                     VGNALGRFDTIFLSDLLISNPSTSAASITFSYFPLDPSEPPKTQRFTLGAGESHAFEF
                     LLPTIFQVDPAQGAIRVDSDVPVVSAVRIAARKPEGDFATLALPIGSDAAVTTGSGNA
                     FAIQTASRRTNLLLFNGGAAGTVTVRAINGNNDEIGHLSVEIGSLESVRLDSILTSLG
                     AGEEPNGRLVVQGSDGMQLYAWAAQVDGPTGDVEIEALRP"
     gene            6714..7787
                     /locus_tag="VF958_06520"
     CDS             6714..7787
                     /locus_tag="VF958_06520"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011984331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0033818 -
                     beta-ketoacyl-acyl-carrier-protein synthase III activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HEX9148800.1"
                     /translation="MGSPTTVIRGSGMFVPEGVVTNERMSLLMDTNDEWIRQRTGIGE
                     RRYARKGTSSSDLAVEAARRALDDAGLEARDLEFIVFATMTPDHYFPGNGGLLAKKLG
                     LETTHALDVRMQCAGFLSGLQVADAFIRSGTYRRVLLVGAECHASLYPWTDAEWAVMF
                     GESDGPVSPKAYAWGTAQRDRAVIFGDGAGAFVLEANDSGDGRGFLGFEMRTDGRHWD
                     KLYVPGGGSASFPYFSPEMFENDGTVPIVEGRQVFRLATTMMPGIVREVLAKQGLAIE
                     DLDLLLMHQANLRINEAVQKHLALSDEKVFNNIQRYGNTTAATLPLVFHEAKAAGRVK
                     PGSLVCFTALGAGLHWGAGLVRI"
     gene            7854..8294
                     /locus_tag="VF958_06525"
     CDS             7854..8294
                     /locus_tag="VF958_06525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9148801.1"
                     /translation="MRGVAVFALLIGACLLPLGCGDEKPLPTDPGGGPGPSPLATYTR
                     VQNEVFTLKCALSGCHLGPASAAQEGLVLSSDVAYDNIVMVRSNQNSSIFRVTPGDPT
                     NSYLWRKINPGQPIVGERMPQTGSITESERQLVTDWIQRGAPRD"
     gene            8291..9163
                     /locus_tag="VF958_06530"
     CDS             8291..9163
                     /locus_tag="VF958_06530"
                     /inference="COORDINATES: protein motif:HMM:NF040196.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5777 family beta-barrel protein"
                     /protein_id="HEX9148802.1"
                     /translation="MKSGFGTRRSRRRLVAILLLGAAPLLAQDAPTSTPPRAPEGSRI
                     INLPSVEVPAAGNLGVLFFHRFKQALGESSVRDFFSLDSGADTVLGVSYSPLDRLEVS
                     LDRSSIEADFELSVKYRVLSMQENRPFALAVRVGGDAVTKENVENREGFFAQGIASIA
                     IGSRIRATVVPTYVSETALFQDVFSAPVALSISITRTVNVHGEVYPKNRDFTETPSRR
                     AGPGVTPRPGRQTHIGWNASIEKTVLRHRFAFTVGNMRGSNVDQYTASDLGGAGIPHD
                     VYLGFNLVRQWKLK"
     gene            9299..9829
                     /locus_tag="VF958_06535"
     CDS             9299..9829
                     /locus_tag="VF958_06535"
                     /inference="COORDINATES: protein motif:HMM:NF027338.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate-binding family 9-like protein"
                     /protein_id="HEX9148803.1"
                     /translation="MRVGFRDGSLLVRFDGRDDGTVATLTRRDDPLWTEDVFEVFVTP
                     LDPPVVYFEFEVNPLGTLFDARVTSPDLARATMNLDVAWNLPGLRATSRVRPGLWSAT
                     LRIPLAPMLGDVSALTPSLSRGEREDARHRRPWRANFYRVDRGERDEFSAWSSTGKEP
                     ADFHEAVRFGRLTLPI"
     gene            complement(9826..10254)
                     /locus_tag="VF958_06540"
     CDS             complement(9826..10254)
                     /locus_tag="VF958_06540"
                     /inference="COORDINATES: protein motif:HMM:NF015629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0158 family protein"
                     /protein_id="HEX9148804.1"
                     /translation="MPSPHLELDWEGLVVAFESRSGQITHFFDRETGDVVQVLERDAE
                     RHAAMAADPRYAALPRDSGERSRGDLEDFVATCEEAGCRRDLSAALAEPDPVAAYRAA
                     LLLHPKEEARFFQFKQRRARERAEGWLAGQGIPFEREVRR"
     gene            10286..11398
                     /locus_tag="VF958_06545"
     CDS             10286..11398
                     /locus_tag="VF958_06545"
                     /inference="COORDINATES: protein motif:HMM:NF016993.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Phenylacetic acid catabolic protein"
                     /protein_id="HEX9148805.1"
                     /translation="MKKIATFDDWIDAFREWQKDIGLHLPKYADYQFDAKYGDLKTNE
                     IEFGDFKGRDKWERVAQIPHQPIRDALLNLIVYQGDTEFASVEQQRNLLETSPSEYDT
                     DSVCRIMVEEMRHGWQMCHLLVSDFGETGKIEARKLLERRSFQNNRLLGSFNVDVDNW
                     VDFFTYTEFVDRDGKFQLNMLSTSAFAPLARSMGPMLKEEGFHLGTGHMGLQRIASAG
                     RVPAPLLQKYINKWLSTAYDLFGKDHSSSAEWFYVWGLKGRYDEHRHAGAPDRERLNE
                     LSREMYKLEVDKLIEQINKHLPPGANPIVAPDMKFHRGIGEFAGKTYSVAGEPLSAED
                     YQKHLAETLPTPEDQQRIASLMREPGWIAPRESVTA"
     gene            11503..12453
                     /locus_tag="VF958_06550"
     CDS             11503..12453
                     /locus_tag="VF958_06550"
                     /inference="COORDINATES: protein motif:HMM:NF012933.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HEX9148806.1"
                     /translation="MLQQTTVAAVRKRYDGFLLRFPDVESLARATEEEVLAAWSGLGY
                     YARARNLRLAAQAIVRNHGGSIPREPAALRRLPGFGPYMAAAVASLAYGARVPAAEAN
                     VERVLSRLFALSGLSGSPALRARVLAVAQELLPARRPGDLTAALMDLGQTVCTPRRPI
                     CPVCPVASACEARRRGDPERFPQRRKKASPVHVSLAAALAERRGRFLLVRRRSSWLDG
                     LWEFPSAEGASTEEARRRLVHRVAALGLEVSASPAIGSARHTVVNRRIEISVFRADRA
                     SRPGARTKRGAGRWFLPGELADAAVPTLTRKIAAAGLRRG"
     gene            12483..13271
                     /locus_tag="VF958_06555"
     CDS             12483..13271
                     /locus_tag="VF958_06555"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEX9148807.1"
                     /translation="MRIEWGGSAADIRELGSGPAIVLVHGFPLDGAMWSGVARALAPR
                     FRVFKPDLPGRGNTDAAAPDSIAGYADFIEALVTDLPPPVGIAGFSFGGYISLELMKR
                     RPEKIRALALIDTRAAADDPAGRARRDETIAAVRAEGVKSVVESMPPKLLSPASLGRP
                     DLLERVQRMISRQKPETLAADLVAMRDRPDSTEFLPRVAVPTLVVVGEQDALTPPADS
                     EAMAAAVPGARLVRIPGAGHLSPMERPKAVAEALADFFSSTLIS"
     gene            complement(13268..>14677)
                     /locus_tag="VF958_06560"
     CDS             complement(13268..>14677)
                     /locus_tag="VF958_06560"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEX9148808.1"
                     /translation="QVTIFDNSTDPARPIYQHFYWTTPTATLTPFNAPISQLNASPEH
                     KIWTAYLQDRWSLFPELTVNLGVRWDRQEIIDASGASRITLDKDFAPRLGIIWNPGRD
                     KKTKVFGSYGLYYEEIPMDLVIRSFSFERQPNVNNFDPTGIVPDPQADAILDSPTNIL
                     GGFTEPSDPNIKNQYVREIILGVEREIMPDVAVGLKYIYRDYGRVIEDFLCMDDGTYC
                     IGNPGEGIMKNIFTLDYATTFPAPDPQRIFRGVELDVSKRFSGNWQMMASYLWSKLSG
                     NYDGEFAPFTNVGPDPNISAAYDYYDFFTNGQNRDVITNRGPLSNDRRHQFKISGVYV
                     TDFRLSLGLSAYYRTGTPLTRYGFSDAYGRYEFFLTRRGAEGRAPDNYEADFHVGYPV
                     RIGPITVNLLADVFNLLNAQRPILLDQRYNFAEFANADYVCGSNLSTVDEPKCNERYG
                     QAFLRQAPRSLRLGARVSF"
BASE COUNT         2365 a         4948 c         4864 g         2500 t
ORIGIN      
        1 cgtcctcgtc tccgagatac gttccgagtc cgatcgagga gagacggacg ccggcggcgt
       61 cccggaaatg tcctgccgct gccttcgcgc gatccgcgaa ccgcttcgtg ccgtcgagag
      121 tggcgcgccc gggagtcacc cgggaaaggt atcagctcga ggctcaggcg tacgcgcgat
      181 tgacgaaccc caggtcggga tatccggcgc ccaggatcgc cgccgcgtcc gcgccgagcc
      241 agtcctgcag gatcgtgccg tagtacgaac ggaaatcgtt ctgaatcgcc cggttcccgt
      301 tcgagtcgag atgcgagagg tccggcggat tcccgtagat ccccggcagg accgcgttgc
      361 cgacgacgaa gtgagctccg gcggtgccgt gatcggtgcc gttggaaccg ttgtcctggg
      421 ggcggcggcc gaactccgac cacgtcatca agaggacgtc gtgcgacttg ccgtgggcgt
      481 cgaggtcgtc gaagagagcc ttgattccgt tcgagaacgt cgtcaggagc ggcggctgat
      541 cggtcgtctg ggtcgcatgg ttatcgaagc cgccgaacgt gatgtagaga atccgcgcgc
      601 cgacgttcgc gttgacgatc tgcgcgatga gaaggagtcc cgcgccgagc gtccccgccg
      661 gataggtggc gccggcaacg taccccgtgc cgacggtctg gaggatgttc gaggagctga
      721 tcgcatcctc gcccacccgg gacagcgcat cggcggtgtt cgggacttcg tactcgccgc
      781 cgttctgggt caggaacgtg cggatctggt tgttgcggtc gccggcgtac tggccgtcgg
      841 tcgcgtattg atagctcgcg aggttgacga atgacggcac gccgacgttg tccgcgagca
      901 gggtccgcgg aagcgactgc gagatcgccg cacacgagag tgggttcgag ctgcccgcca
      961 cgtaccggtc cgcccagcgc ccgagccagc cgtgcctctg cgcgagctgt ggatcggcgg
     1021 tgtgccacac gtcccgcgcg gcgaagtgcg agaggctcga gttcgggtat ccgacgttct
     1081 cgacgaccgc gagccggttg gcgtcccaga gcgatttcat tccggtcatg accgggttca
     1141 ggccgagacg gccgtcgagc ggcagcaccg taccggccgc gacgccgatt cgcgagcgca
     1201 gctggagata gaggttgtcg gtgtacggca caaccgtgtt caggtcgtcg ttgccgccgg
     1261 cgagctcgag gacgacgagc gtccggttca cgaaatccgc tccgaccgac tcctcgaagc
     1321 gacgcgcacc ctcgaccgcc cagcgcggca ccatcgccga caccgtcacg aaccccgaag
     1381 ttcggaggag gaagtcgcgg cgggagatcg tcatggcggc ccccgatcta tgccaggtgg
     1441 aaagccggac cggtcagcat gaggtggatc agccctcgga ccttcttgtc gacgttcgcc
     1501 gacgtattcg tccaggttcc tcgcgacccg tcgtcgttcg cgttcatgta ctcgatccag
     1561 gaggcgcgaa ccctcgcgga gacgtccgcg acgttgaagc ggtcggcgag gatgtcgacg
     1621 actttctcgg cggtcgacgc atcccgtccg gcgagcagcc gggccggatc gaaacgcgtg
     1681 ccggtcgtcc cgcggttcgt gcagatcgtg ttcacggtcg tcgcccgcgc gaagagagtc
     1741 cccgtgttca tccaggactc gccccactcc caacccttcg cgtcctccgg gttgaacagg
     1801 atctgcccca tcgccgcgag ttggcggact gcgacgtcga agtcgctcgt cgcctcggtc
     1861 aggcggatcg ctgcgacggc atactcgacg gggctgcgca cccacgtctt cctcgtgtgg
     1921 gcctcgtaga actccgggtg caggaggatt cgccgcacga cctcgcggat cgagtggttg
     1981 ctcgaaacgt agacgtcgcg cagctcgtcg accacgtgcc gcggcggcgc ctcgtaggcg
     2041 aagaacgtcc agagcttcgc gccgatgaac ctcccggaga ccgagccttc ggagtcggaa
     2101 tgatcgagga tgatccggat cgcgtcggcg ccgtcccagg cgccggtctg cccgaggatc
     2161 gtcttcgacc cggtgtcgtg gaggctcggg ttctccagga aggtcgcgcc gttgtagccg
     2221 tccgaacccc ggtagttctg gagcgtccag cccgtcaggg cgcgcgcgac ctcgcgcacg
     2281 tcggtctcgg agtagttgcc gatccccagc gtgaagagct ccatgagctc gcgcccgtag
     2341 ttttcgttgg ggcggccggc gcggttcagc cagttgtcga gccagacgag catcgccgga
     2401 tcctgggaga tcttcagaac gagatcgtcg aaccgcgcga ccgcgtattg ccgctccgtc
     2461 ttgttctggt tgaggatcaa cgtcacgccc tggaccttcg cgaacccgga ggtccagtgg
     2521 agattccaga agtagttcat cttctcttcg agcggccgcg gggagaaggc catgcggtcc
     2581 aggaaccact gctggacgcc gcggatcgtc gtgacgttgt agttcttcgc ggcgaggctc
     2641 gcctcgtacg ccgtcaggct gaccgagtcg taatcgacga gccacgagac cgcgccctcg
     2701 agtccgcgcg cgtagaggtc gtcgatctcc gcgggggtgc cgccgaaacc ggctcggcga
     2761 gaaaggtggg cagcggcctc ccgggtccag tcgacggtca cgagccctcc ttccgaaagg
     2821 gcgtcaactg acaggataga cgagaccggg cgggagcggt ttaccgccgg ctcagaacac
     2881 gggcaacggt atgccgtgcg tcttcgtcgt cccctccatc cgcgggtccc agcggccggg
     2941 gatcggcgtc tggcagcgga agcaggcgcc gtcggcgaga cggttcttcc gcacgaggaa
     3001 gccgtaacgc tcgatgaggg tcgtggcgca acccgggcac cgggtgtcct cccactttcc
     3061 cacgcgaccc ggcaggttcc cggcgtacac gtaccgcaag ccctgctcgg tcccgatctc
     3121 cgccgcacgg acgagcgtgg aaaccggggt ccccccccgg tcggtcatct tgtaatcggg
     3181 atggaaggcc gtgacgtgcc aggggatgtc cggcgagacc gaggcgacga acgccgccgc
     3241 gcgggtcagc tcctcttcgg agtcgttgaa gcccgggatg acgagcgtca gcacctcgag
     3301 ccagatgccg cggtcgtgga tcccctggat cgtgtcgagc acgttttgca gaacgccgcc
     3361 gagctcgcgg tagtgcttgt cgtccatgga tttcaagtcc accttgtaga agtcgatcca
     3421 gggccggatg tagtcgagca cctgcggggt gccgttgccg ttggaaacgt atcccgtgga
     3481 gagccctcgc tgcttcgctt cgcgaaaaac ggcgaccgcc cactccgacg tgatgagagg
     3541 ctcgttgtat gtcgagacga ccgtcggcgc gccgcactcg accgcgagct ccgcgagccc
     3601 ttccggcgag acgtcgcggg ggggagcgac cgcctgcgga tcgcggatcg actgagaagt
     3661 gaaccagttc tggcagtacc cgcagtgcag atcgcatccg agcatgccga aggagagcgc
     3721 gtcggagccg ggcaaggcgt ggaagaacgg tttcttctcg atcgggtcgc actggatcgc
     3781 gccgacgtag ccccacggaa ccttgagcac gcccttctcg ttgtaacgga ctcggcacac
     3841 cccttcgcgg ccttcgggga tcgggcagcg gtggccgcac gaaaagcact tgagacgccc
     3901 gtcgggcagc cgctcgacga gctcgggcgc ggccgcaatc gttcggcggg agagcacttc
     3961 cgcgacggga gacttcggca gaggtgcgcg ggaggccatg cggaaaagat aacgccggga
     4021 ggcccgtgcg gaattgcccc tcaccccgac cctctccccg caggcgggga gagggagtgc
     4081 ggcgtcggcg tgtcgcgcct ctcacaaacg agaaatgtcg cgcgctgccc ggaggttatg
     4141 atccgccgat gcccaaatcc ctgcgtctgt cggggctcgt cgccgcggtg agcctgctct
     4201 cgcccctcct gcgggcgcaa accgacaacc cgaccacgat tcccacggga aacgcctacg
     4261 agaccgagaa gtcgggggtc gtgagcggag tgcggataga cgtacggccc gacggttcgg
     4321 tctggttcct ggtccccgct ctcgaccgga tcgcggttct ccggggcgga gagacgaagc
     4381 agtgggcgct ccgcgacgac aaccatctcg gcgcgaaccc cgtggacttc gagatcgacg
     4441 gtgactccgt gtggttcatc tgcaatggag agagcggggt cgacgcaggc cattcgatct
     4501 tcggccggct cgacaccgta acggggcagg tccgcgagtg ggaggtgccc ggctcgcgtc
     4561 cagccggctt ctatcgagcc ctcgacggca aggtctggat tccgcagacc gaccggcggc
     4621 tccagtcggt cgatctgacg accctcgagg tcgtcgacta ccggtccccc cagacgatcg
     4681 cgtactccga catcgccgtt ggtcccgacg gcgctttctg gatgacggac ttcggaaaca
     4741 accggatcgt gcgcttcgag cccggcgcgt cggaggagac ctcctggacg acgttcgatc
     4801 cggcgctcgc gatcctgaac ccctcccaga tccagttcga cgcccagggt cggctctgga
     4861 tctccgagtt caccggcgcg cgcatggacc gtttcgaccc ggccacgggc gatctcgtca
     4921 gcttcgtcgg gttcctgaac ccgattcact tcgacctgtt cgggggccgc gtttacgtga
     4981 ccgaagccca cggagggaac ggccagatcg ctctcctcga cccgtccttt gcgacatccg
     5041 gcaccacgac tctcacgtcg cagacgttcg acgtcggctc gctcgtcaac gtccggcagg
     5101 cgacgatcca cgaccacacg gtcacaccga cgacgtttgc ctcccccgcg acggcgatcc
     5161 ccgcggcgga cctgaaggtc gcggccggcg cgcccggtgt tctccgcacc gagattccat
     5221 ggcagaactc ctacggaatc gacgtcgtcc gcggagaggt ctgggtcggc acggccgggc
     5281 ggctcgccca cgtggagctc cagaccgtcg gcaccccctc ggacctcgtg gctcccgtgg
     5341 cgacccagct cgccggcgac gccacgcacg agtcccgcgt cgacctgacc tttcacaacg
     5401 cgagtgacga aacgatctcc ggggaacttc tctatctcta ttcccccggg ttcttcgcgg
     5461 cgcgtcagac cttctcgctc ggtcccgggg cgacgtcggt cgtctccgat gcgttcggcg
     5521 acctcgggtc gtcgacgatc cccgtcacgg gcccgatccg ggttcacgtc acctccgggc
     5581 ccgccgacgc gctcgaggtc agcgtccgga ccgcccaggc gcgcgaagac ggcggctcct
     5641 tcggatactc gacgcccgcc ttctcgccgg ccgagagcct cggcgcgggg gccagccgaa
     5701 ccctgttcac cgggttccgt gagtcggaga cgtcgatctt cgggttctac agcccctcag
     5761 gagccgaggc ggttttcacg ctcgtggctc ccgacgggac ggtgcggggc gagcttccga
     5821 tctccatcat ttcgaacatc gacgaggagt tctcgccggc ggcatcggct ttcggcgttc
     5881 cgccagagcc gggggacgtg atccgcgtct ccgtcgcatc cggaacgctc ctcccctggg
     5941 tccgcatcgt cgacggcggc tcgaaggaca ccgcgctgtc gcttcccgct cgcgagctcg
     6001 cgagcgcgac cttcccgaac gtcggcaacg ctctcggtcg cttcgacacg atcttcctct
     6061 ccgacctcct gatctcgaat ccctcgacct ccgcggcgtc gatcacgttt tcctatttcc
     6121 ccctcgatcc ctccgagccg ccgaagacgc agagatttac gctcggcgcc ggagagagtc
     6181 acgccttcga gttccttctt ccgacgattt tccaggtcga tcccgcgcag ggggccatcc
     6241 gcgtcgactc cgacgtcccg gtggtctccg cggtgcgcat cgccgcccga aagccggaag
     6301 gtgacttcgc cacgctcgcg ctcccgatcg ggagcgatgc cgccgtcacc accggatcgg
     6361 gcaatgcgtt cgcgatccag acggcgtcgc ggagaacgaa cctgcttctc ttcaacggcg
     6421 gggcggccgg gaccgtcacc gtccgggcga tcaacggaaa caacgacgag atcggtcatc
     6481 tctccgtcga gatcggatcg ctcgagagcg tccggctgga ctcgatcctg acgtcgctcg
     6541 gggcgggaga ggagccgaac ggccgtctcg tggtccaggg ctccgacgga atgcagctct
     6601 acgcctgggc ggcccaggtg gacgggccca cgggagacgt ggagatcgag gccctccgtc
     6661 cctagtccgg aaggcagccc gccggcgcgc gggtgcatat gattccgggc gccatgggat
     6721 ctccaacgac cgtcattcgc ggttccggga tgttcgtccc cgagggcgtc gtcacgaacg
     6781 agcggatgtc cctcctcatg gacacgaacg acgagtggat ccgtcagcgc accggaatcg
     6841 gcgagcggcg ctacgcgcga aagggaacgt cgtcttcgga cctcgccgtc gaagcggcgc
     6901 ggcgcgctct cgacgacgcc gggctcgaag cgcgcgacct cgagttcatc gtcttcgcga
     6961 cgatgacgcc ggaccactac tttccgggca acggcgggct gctcgcgaag aagctcggtc
     7021 tcgagacgac ccacgccctc gacgtgcgca tgcagtgcgc cgggttcctc tcgggactcc
     7081 aggtcgccga cgcgttcatc cggtcgggca cgtaccggcg tgtgctcctc gtgggcgccg
     7141 agtgccacgc gtcgctctac ccctggaccg acgcggagtg ggcggtgatg ttcggggaat
     7201 ccgacggacc cgtctccccg aaggcgtacg cgtgggggac cgcccagcgc gaccgggccg
     7261 tgatcttcgg cgacggcgcc ggcgccttcg tcctggaggc caacgactcc ggcgacggcc
     7321 ggggctttct cggattcgag atgcgcacgg acggacgcca ttgggacaag ctctacgttc
     7381 cgggcggcgg ctcggcctct tttccgtact tctcgcccga gatgttcgag aacgacggaa
     7441 ccgttccgat cgtcgagggg cggcaggtgt tccgcctcgc cacgacgatg atgccgggaa
     7501 tcgtccgcga ggttctcgcg aagcagggac tcgcgatcga ggacctcgat ctccttctca
     7561 tgcaccaggc gaacctccgc atcaacgagg cggtccagaa gcacctcgct ctctccgacg
     7621 aaaaggtgtt caacaacatc cagagatacg gcaacacgac agccgcgacg ctgcccctcg
     7681 tctttcacga ggcgaaagcc gcggggcgcg tgaagcccgg ttccctcgtc tgcttcaccg
     7741 cgctcggcgc cggcttgcac tggggcgcgg ggctcgtaag gatctagcga cccggctcaa
     7801 gactttctga aggttcgccc gggcaacgac ccatggctat actccgccga gtcgtgcgag
     7861 gggtagcggt tttcgctctt ctgatcggtg cttgcctcct tccgctcggg tgcggcgacg
     7921 agaagccgct gccgaccgac cctggcgggg gccccggccc ttcgccgctc gcgacctaca
     7981 cgcgcgttca gaacgaggtc ttcacgctga agtgcgcgct ctccggctgc catctgggac
     8041 ccgcttccgc ggcgcaggag gggctcgttc tctcctcgga cgtggcctac gacaacatcg
     8101 tgatggttcg atcaaatcag aactcgtcga tcttccgcgt gactcctgga gatccgacca
     8161 actcctacct ctggcggaag atcaatcccg gtcagccgat cgtcggcgag cgaatgccgc
     8221 agacgggatc catcaccgag tcggagcgac agctcgtgac cgactggatt cagagaggag
     8281 caccacgaga ttgaagagcg gtttcgggac tcgacgttcg aggaggcggc ttgtcgcgat
     8341 ccttctcctg ggcgcagctc cgctgctcgc tcaggacgcg ccgacctcca cgccaccgag
     8401 ggcgccggag ggatcccgca tcatcaactt gccttcggtc gaggttccgg cggcggggaa
     8461 cctgggcgtt ctgttctttc accggttcaa gcaggcactc ggcgagagca gcgttcgcga
     8521 ctttttctcc ctcgattccg gagccgatac ggtcctgggg gtttcctact cgccgctcga
     8581 ccgcctggag gtctcgctcg atcgttcgtc catcgaggcg gacttcgagt tgagcgtcaa
     8641 ataccgcgtg ctttcgatgc aggagaaccg acccttcgcg ttggccgtgc gcgttggagg
     8701 cgacgcggtc accaaggaaa acgtcgagaa ccgcgagggg ttcttcgcgc aggggatcgc
     8761 gtcgatcgcg atcggttcac ggatccgcgc gacggtcgtc ccgacgtatg tttccgagac
     8821 ggcgctcttc caggacgttt tcagcgcacc ggtcgcgctc tccatctcga tcacgcgcac
     8881 ggtcaacgtt cacggcgagg tctacccgaa gaatcgagac ttcaccgaga ctccctcccg
     8941 aagggccggt cccggcgtca cgccgcgtcc cgggcgccag acccacatcg ggtggaatgc
     9001 ctccatcgaa aagacagtcc tccgacaccg gttcgcattc accgtcggaa acatgcgggg
     9061 gtccaacgtg gaccagtaca cggcctccga tctcgggggg gcgggaattc cccacgacgt
     9121 ctatctcggt ttcaacctcg tccggcagtg gaagctgaag tagcctccac tcacgaacga
     9181 agccggactt ccccgactcg acgtcccgct gctcggcccc accgatcggc tcgtctcgat
     9241 ccgaccgatc gagctctctg atgcgatcac cggggcaccg ccttcgctgc gcacccgtgt
     9301 gagagtcggt ttccgcgacg gatcgcttct cgttcggttc gacgggcggg atgacggcac
     9361 agttgccacc ctcacccggc gcgacgatcc actctggacc gaggacgttt tcgaagtctt
     9421 cgtgacgccg ctcgaccctc ccgtcgtcta cttcgagttc gaggtcaacc cgctcgggac
     9481 ccttttcgac gcccgagtca cgtcccccga cctcgcgcgc gcgacgatga acttggacgt
     9541 cgcctggaac ctgccgggac tgcgggccac gagccgggtg cgcccggggc tctggtcggc
     9601 gacgctcaga attccccttg caccgatgct cggcgatgtc agcgccctca ccccgtccct
     9661 ctcccggggg gagagggagg atgcacgcca ccgccgcccg tggcgcgcga atttctatcg
     9721 ggtcgatcgc ggagagaggg acgagttctc ggcgtggtcg tcgaccggga aagagccggc
     9781 ggacttccat gaggcagtcc ggttcggccg gctgactctg ccgatctagc ggcgaacctc
     9841 tctctcgaac gggattccct gccctgcgag ccatccctcg gctcgctccc gcgcccgcct
     9901 ctgcttgaac tggaagaaac gcgcctcttc cttcggatgg aggaggagcg ccgcgcggta
     9961 ggccgcgacg gggtcgggtt ccgcgagcgc agcggagaga tcgcgccggc aacccgcctc
    10021 ctcgcacgtg gcgacaaagt cttcgagatc tccgcgcgaa cgctccccgg aatcgcgcgg
    10081 cagcgcggcg tatcgcgggt ctgcggccat cgcggcgtga cgctcggcgt ccctctcgag
    10141 tacctggacg acgtcgcccg tctcccggtc gaaaaagtgc gtgatctgtc ccgagcggct
    10201 ctcgaacgcg accaccaggc cttcccagtc gagttcgagg tgaggagatg gcataaaccg
    10261 ctatgatatg gagtttgagg tacttgtgaa gaaaatcgcg acgttcgacg actggatcga
    10321 cgccttccgc gagtggcaga aggacatcgg cctgcacctc ccgaagtacg ccgactacca
    10381 gttcgacgcc aagtacggcg acctgaagac gaacgagatc gagttcggcg acttcaaggg
    10441 ccgggacaag tgggagcggg tggcgcagat cccccaccag ccgatccgcg acgcgctcct
    10501 gaacctcatc gtctaccagg gggataccga gttcgcgtcg gtcgagcagc agcgaaacct
    10561 cctcgagacc tccccttccg aatacgacac cgacagcgtc tgccggatca tggtcgagga
    10621 gatgcgtcac ggctggcaga tgtgccacct gctcgtcagc gacttcggcg agaccgggaa
    10681 gatcgaggcg cgcaagctcc tcgagcgccg atcgttccag aacaaccgcc tgctcgggtc
    10741 tttcaacgtc gacgtggaca actgggtcga cttcttcacg tacacggagt tcgtcgatcg
    10801 cgacggcaag ttccagctga acatgctgtc gacctcggcg ttcgcgccgc tcgcccgctc
    10861 gatgggaccg atgctgaaag aggaaggctt ccacctcgga acgggccaca tgggactgca
    10921 gcggatcgcg agcgccggac gcgttccggc gccgctcctt cagaagtaca tcaacaagtg
    10981 gttgtcgacg gcgtacgatc tcttcggcaa ggaccactcc tcttccgccg aatggttcta
    11041 cgtttggggg ctgaagggcc ggtacgacga gcaccggcac gcgggcgctc cggaccgcga
    11101 gcgcctgaac gagctctccc gcgagatgta caagctggaa gtggacaagc tgatcgagca
    11161 gatcaacaag caccttccgc ccggcgcgaa cccgatcgtc gcccccgaca tgaagttcca
    11221 ccgcgggatc ggagaatttg ccgggaagac gtactcggtc gccggggagc ccctgtccgc
    11281 cgaggactac cagaagcacc tggcggaaac gctgccgacc cccgaagacc agcagcgcat
    11341 cgcgagcctg atgcgcgaac ccggctggat tgcgccgcga gaatcggtga cggcgtaacc
    11401 tccgggctgc gtcgcgatct cctcgactgg ttccgtcggg aggagcgcga gctcccgtgg
    11461 cggggaagcg cggacccgta tcgagtctgg gtctccgagg tcatgctcca gcagacgacc
    11521 gtcgccgcgg tccgcaagcg ctacgacggg ttcctgctcc gcttccccga cgtcgagtcg
    11581 ctcgcacgcg ccaccgagga agaagtcctc gcggcgtggt cgggtctcgg ctactacgcg
    11641 cgggcccgca atctccgact cgcggcgcag gcgatcgtgc ggaatcacgg aggatcgatt
    11701 ccccgggagc ccgcggctct ccggcgtctg ccgggcttcg gaccgtacat ggccgccgcc
    11761 gtcgcgtcgc tcgcctacgg tgcgcgcgtt cccgcagcgg aggcgaacgt cgagcgcgtc
    11821 ctctcgcgcc tcttcgcgct ctccgggctc tcgggctcgc ccgcgctccg cgcccgcgtt
    11881 ctcgccgtcg ctcaagagct cctcccggcg cggcgccccg gcgacctgac cgccgcgctc
    11941 atggatctgg gacagacggt gtgtacgccg cggcgcccga tctgtccggt ctgcccggtc
    12001 gcgtcggcct gcgaggcccg gcggcgcgga gacccggagc ggttcccgca gcggagaaag
    12061 aaggcgtcgc cggtccacgt ctctctcgcc gccgcgctcg ccgaaaggcg cgggaggttc
    12121 ctcctcgtga ggcgccgctc gagctggctc gacggcctct gggaattccc ctccgccgaa
    12181 ggcgcgtcaa ccgaggaagc gcgcaggcgc ctcgtccata gggtcgcggc gctcggcctc
    12241 gaagtgtcgg cttcccccgc gatcgggagc gcgcggcaca cggtcgtcaa ccggcgaatc
    12301 gagatctccg tcttccgcgc ggatcgagcg agccgtccgg gcgctcgcac gaaacggggc
    12361 gccggccgct ggtttctgcc gggagagctc gcggacgccg cggttccgac cttgacgcgc
    12421 aagattgccg ccgccggact gcggcgcgga taagattccc gcgcacatcg gagacgacga
    12481 cggtgagaat cgagtggggc ggcagcgccg ccgacatccg tgagcttggc agcggccccg
    12541 cgatcgttct cgtgcacggc ttccccctcg acggagcgat gtggtcgggc gtcgcccgcg
    12601 cgctagctcc caggttccgc gtgttcaagc ccgatctccc gggccgcggg aacaccgacg
    12661 ccgcggcgcc ggactcgatc gccggatacg cggacttcat cgaagcgctc gtcacggacc
    12721 ttccgccgcc ggtcggaatc gcggggttct cgttcggcgg ctacatctcg ctcgagctca
    12781 tgaaacgccg cccggagaag atccgggcgc tcgcgctcat cgacacgcgc gcagcagcgg
    12841 acgatccggc cgggcgcgcc cggcgcgacg agaccatcgc cgcggttcgc gcagaaggcg
    12901 tcaagtcagt cgtcgaatcc atgccgccga aacttctgtc tcccgcgtcg ctcggacggc
    12961 cggaccttct cgagcgcgtg cagcgcatga tctcccgcca gaagcccgag accctcgcgg
    13021 cggatctcgt tgcgatgcgc gaccggccgg actcgaccga gttcctgccg cgcgtcgcgg
    13081 ttccgacgct cgtcgtcgtc ggcgagcagg atgcactgac gcctcccgcg gactcggaag
    13141 cgatggcggc cgcggtcccg ggtgcgcggc tcgtgcggat tcccggcgcg gggcatctct
    13201 cgccgatgga gaggccgaag gccgtcgccg aggccctggc cgacttcttt tcctcgaccc
    13261 tgatctccta gaaggagacg cgcgccccca gtcgcagcga ccggggcgcc tgccgcagga
    13321 acgcctgccc gtatctttcg ttgcacttcg gctcgtcgac ggtcgagagg ttggacccgc
    13381 agacgtagtc cgcgttcgcg aactccgcga agttgtagcg ctggtcgagc aggatcggcc
    13441 gctgggcgtt cagcaggttg aagacgtccg cgagcaggtt caccgtgatg ggcccgatcc
    13501 gcacggggta tccgacgtgg aagtccgcct cgtagttgtc gggcgcgcgg ccctccgctc
    13561 cgcggcgggt gaggaagaac tcatagcggc cgtacgcgtc ggagaaaccg tagcgcgtga
    13621 gcggagtgcc ggtgcggtag tacgccgaga gcccgagcga cagccggaag tccgtgacgt
    13681 acactccgga gatcttgaac tggtgacgcc ggtcgttcga gagcggccca cggttcgtga
    13741 tcacgtcccg gttctgcccg ttcgtgaaga agtcgtagta atcgtaggcc gcggagatgt
    13801 tcggatccgg accgacgttg gtgaacgggg cgaactcgcc gtcgtagttc ccggaaagct
    13861 tcgaccacag gtaggacgcc atcatctgcc agttgcccga gaagcgcttc gaaacgtcga
    13921 gctcgacgcc gcggaaaatc cgctgggggt cgggagccgg gaaggtcgtg gcgtagtcga
    13981 gggtgaagat gttcttcatg atcccttcgc ccgggttccc gatgcagtac gtgccgtcgt
    14041 ccatgcagag gaagtcctcg atgacccggc cgtaatcgcg atagatgtac ttcagaccga
    14101 ccgcgacgtc cggcatgatc tcccgctcga cgccgaggat gatctcgcgc acgtactggt
    14161 tcttgatgtt cggatccgac ggctcggtaa agcctcccag gatgttcgtc ggcgagtcca
    14221 ggatcgcgtc ggcctgcggg tccggcacga tcccggtcgg gtcgaagttg ttcacgttcg
    14281 gctgacgctc gaacgagaac gaacggatga cgaggtccat cgggatctcc tcgtagtaga
    14341 ggccgtagga cccgaagacc ttcgtctttt tgtcccgccc gggattccag atgatcccga
    14401 gccgtggcgc gaagtccttg tcgagcgtga tgcggctggc gccggacgca tcgatgatct
    14461 cctgccggtc ccaccggaca ccgaggttca ccgtcagctc tgggaagagg ctccatcggt
    14521 cctggaggta cgcggtccag atcttgtgct cggggctggc gttcaactgg gagatcggcg
    14581 cgttgaacgg agtgagcgtc gccgtcggag tcgtccagta gaagtgctgg tagatcgggc
    14641 gagccgggtc cgtcgagttg tcgaagatgg tgacctg
//
LOCUS       DASWAV010000320         5395 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U2665
            k141_696824, whole genome shotgun sequence.
ACCESSION   DASWAV010000320 DASWAV010000000
VERSION     DASWAV010000320.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960316
            Sequence Read Archive: SRR10260007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 5395)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5395)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:47:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,929
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5395
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_696824"
                     /isolate="SMAG_U2665"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.36444 N 132.31889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..155)
                     /locus_tag="VF958_06565"
     CDS             complement(<1..155)
                     /locus_tag="VF958_06565"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008433789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_process: GO:0009235 - cobalamin metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP:cob(I)alamin adenosyltransferase"
                     /protein_id="HEX9148809.1"
                     /translation="MMPRSTRVYTRTGDDGMTSLGGGQRVAKDSLRVEAYGTVDELNS
                     SIGAALAS"
     gene            complement(213..2531)
                     /locus_tag="VF958_06570"
     CDS             complement(213..2531)
                     /locus_tag="VF958_06570"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEX9148810.1"
                     /translation="MTTAVLLVLLGALSRLVPHPPNFVPLGALALYSGARLPRRWSFA
                     VPLAAMAVSDFFLEFGTGRSVLSVDRVAIYATFALLTLLGRAAGDRVRPGRLAGLSVG
                     ASVLFFVTSNFAVWAAGTLYPRTVSGLALCYTAAIPFFRNTLAADLAGTAAFFGLDAL
                     SKRVGARAALRASAAAGMAVVAFGSTALAQTPPPVSDSVVVTATLTPEEERELGSATT
                     VLTREWIEATGARNVADLLRQVPGLDVARQGSDGSLVSAFLRGANSPHVLVLMDGARL
                     NSPYFSGYDFSVLTTENVERIEIVRGPFSALYGSDAIGGVIHIFTRPASTALSGRVAL
                     EGGEAGLKHGSAFATAGSPGFGATGSYSHDELDGDRANSDWRQDAGSVRLEGRFGDRL
                     RVALEGSLVDADVGTPGPVGRETPRARQESREERLQLPVSFRPLEGHDATLLFARVAS
                     ERTYSDPDAGFDSRSNPTTWQARLSDSFHAGRHELTGFTSWERWQVDDRSNFGVALDD
                     DRSTLWGAGFQDSVAVGGGAVATAGLRYDHHSDFGDAWSPRGTAAWTDPTARWKLRVS
                     GGSAFRAPSVGELFYPFGGNPNLKPERVTSWEAGVERYFAVGRLEVSLFWNELRNLIV
                     FDFASSLNENVGRARTRGVEVAWRQKISKAVEVDAGYTWLEAKDRSTGADLRRRPRHR
                     AFLSTALSPIERLTLSPRVTFVGRRGDADALTGIDTQLPSYVRADVFARYDLGRLAPY
                     ARLENATDRRYEEVDGYPSPRRRWSAGMEVRF"
     regulatory      complement(2629..2820)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            2860..3648
                     /gene="larB"
                     /locus_tag="VF958_06575"
     CDS             2860..3648
                     /gene="larB"
                     /locus_tag="VF958_06575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020619151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel pincer cofactor biosynthesis protein
                     LarB"
                     /protein_id="HEX9148811.1"
                     /translation="MTGQDYTLDFDRLRRVGFPEVVLAEGKTAAQIAEICRQLARAHD
                     VLVTRLFPAQWDELSVLPLPGRADYDERSATLHLSVGKPMARLEGNVVLVTAGTADVT
                     VAEETRRTLEYLGVTSTLVPDVGVAGILRLLSRLEQVEQADVVIAVAGMEASLFSVIG
                     GLVGRPLIAVPTSRGYGAALSGVSSLLAALNSCANGVTAVNIDSGYAAAMAAYRILSG
                     GPRRWDRGRKEGSATPASTSTKPRSASTARGSARSSGGRRRPSR"
     gene            3585..4646
                     /locus_tag="VF958_06580"
     CDS             3585..4646
                     /locus_tag="VF958_06580"
                     /inference="COORDINATES: protein motif:HMM:NF014027.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008612 - peptidyl-lysine modification to
                     peptidyl-hypusine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyhypusine synthase family protein"
                     /protein_id="HEX9148812.1"
                     /translation="MGVDRKRKRALLGRPTTPVQVGPKTTVSELLDSYRDASFQARAL
                     GNCAAIFSSMLSDKDRPTIFLGLAGSLIAAGMRQVITDLIEQNMVDVVVSTGAIISQD
                     YYQVRGGRHYHGSVNANDKELRDLYIDRLYDTFIDEEKYWKTDLAVSQFADTLAGKTL
                     SSRAFLALLAESAKKDPGSILGACARNGVPLFVPALNDSSIGIGLTEHYHRMRKDGKQ
                     PMTISSIRDNYELTQIVVRSPATSAVYVSGGVPKNYINDSIVMSYIFGIDTGGHRYAI
                     QITTDAPHWGGLGGSTLSEATSWGKVSKKASHEMAFVETSVALPLLYGYALQKQAHKG
                     RKRLRYAWREDILEKVTRV"
     gene            complement(4763..>5395)
                     /locus_tag="VF958_06585"
     CDS             complement(4763..>5395)
                     /locus_tag="VF958_06585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9148813.1"
                     /translation="KPFVDVAPQQYVRFSVVKGDPASQDVILISEEKGFKPTVAETAQ
                     PYVKAEISPAGDKDKIAGRPGDQYKLNVSVLADAPEGLLNAPVRVTTGVQQQPTLEIP
                     VSGIVRPRVSVSPITVNFGNFTAGKDPITRNIVVTNNKPGTPVKVTKAEVSIPGFMTD
                     VVPTQEGVSYTVVVKASGAVTKGALDGTVKLFTSDKERAVIEIPLRGEVL"
BASE COUNT          886 a         1872 c         1838 g          799 t
ORIGIN      
        1 gacgcgagag cggcgccgat ggagctgttg agctcgtcga cggtgccgta cgcctcgacg
       61 cggagcgagt ccttcgcgac gcgctgtccc ccgccgagcg aggtcatgcc gtcgtcgccg
      121 gtgcgcgtgt agacccgggt actacggggc atcaggtcac gccattctcc ctctccccgc
      181 ctgcggggag agggctgggg tgaggggcga attcaaaacc tcacctccat ccccgccgac
      241 caccgccggc gcggcgacgg ataaccgtcc acctcctcgt aacgccggtc ggtcgcgttc
      301 tcgaggcgcg cgtagggagc gaggcgtccg aggtcgtagc gcgcgaacac gtcggcgcgc
      361 acgtaggacg gaagctgcgt gtctatgccc gtgagcgcgt cggcgtcccc tcttcgtccc
      421 acgaaggtga cgcggggcga gagagtgagt cgctcgatcg gcgagagcgc cgtggaaagg
      481 aacgcgcggt ggcgaggtcg ccgcctcagg tcggccccgg tcgaccggtc cttcgcctcc
      541 aaccacgtgt atcccgcgtc cacttccaca gctttgctga tcttctgccg ccacgcgacc
      601 tcgaccccgc gcgtgcgggc gcggccgacg ttctcgttca gagaggacgc gaagtcgaag
      661 acgatcaggt tgcgcagctc gttccagaag agcgacacct cgagccgtcc gacggcgaag
      721 taccgctcga ccccggcttc ccaggacgtc acgcgctccg gcttcaggtt cgggtttccg
      781 ccgaacgggt agaagagctc gccgaccgac ggcgcgcgga acgcgctccc gcccgacacg
      841 cgcagcttcc accgggcggt cgggtcggtc catgcggcgg tgccacgggg gctccacgcg
      901 tcgccgaagt ccgagtggtg gtcgtagcgc agtcccgcgg tggcgaccgc gcccccgccg
      961 accgcgaccg agtcctggaa accggccccc cacagcgtcg agcgatcgtc gtccaacgcg
     1021 acgccgaagt tactgcgatc gtcgacctgc cagcgctccc acgacgtgaa gccggtcagc
     1081 tcgtgtcgcc cggcgtggaa actgtcggag agccgcgcct gccaggtcgt cgggttcgac
     1141 cgcgaatcga agcccgcgtc cgggtcgctg taggtgcgct ccgaagcgac tcgcgcgaag
     1201 agaagcgtcg cgtcgtggcc ctcgagcggc cggaacgaga ccggcagctg aagccgctct
     1261 tctcgcgact cctgccgcgc gcgcggcgtc tcgcggccga ccggaccggg cgttccgacg
     1321 tcggcgtcga cgagcgaccc ttcgagggcg acccgcagtc gatccccgaa ccgcccctcg
     1381 aggcgcacgg agccggcgtc ctgtctccag tcggagttcg cgcgatctcc gtcgagctcg
     1441 tcgtgggagt agctccccgt ggcgccgaac cccggcgagc cggccgtcgc gaacgccgag
     1501 ccgtgcttga gacccgcttc gccgccctcg agtgcgacgc gcccggacag cgccgtcgaa
     1561 gccgggcgcg tgaagatgtg gatgacgcca ccgatcgcgt ccgagccata gagcgccgag
     1621 aacgggccgc ggacgatctc gatccgctcg acgttctccg tcgtcaggac cgagaagtcg
     1681 tagcccgaaa agtacggaga gttcaggcgc gcgccgtcca tcagcacgag aacgtgcggc
     1741 gagttcgcgc cgcgcagaaa cgccgacacg agagagccgt cgctcccctg ccgcgcgacg
     1801 tcgaggccag ggacctgtcg caggaggtcg gcgacgtttc gcgccccggt cgcctcgatc
     1861 cactcgcggg tcaggaccgt cgttgccgag ccgagctcgc gctcctcctc gggggtcagc
     1921 gtcgccgtca cgacgacgga atccgaaacg ggaggcggag tctgcgcgag cgcggtcgag
     1981 ccgaacgcga caacggccat cccggcggcg gcggaggcgc ggagcgcggc gcgcgcgccg
     2041 acgcgcttcg agagggcgtc gaggccgaaa aacgcggcgg tgcccgcgag atccgccgcg
     2101 agcgtgttcc ggaagaacgg aatcgccgcc gtgtagcaga gcgcgagccc cgagaccgtc
     2161 cgcggataga gcgtccctgc cgcccagacg gcgaagttcg aggtcacgaa gaagagaacg
     2221 gacgcaccga ccgaaagtcc tgcgagccgc cccggccgga cccggtcgcc ggcggctcgg
     2281 ccgaggagcg tcaacagggc gaacgtcgcg taaatcgcga cccggtcgac ggagagcacg
     2341 gatcgccccg tcccgaattc gagaaaaaag tcggaaaccg ccatcgcggc gagaggcacg
     2401 gcgaagctcc accgccgcgg cagcctcgcg cccgagtaga gcgcgagcgc gcccagcggg
     2461 acgaaattcg ggggatgagg aacgagccgg gaaagagcgc cgaggaggac gagcaggacc
     2521 gcggtcgtca ttcgaacctc ccgttcgacg agacgtctct cgcgcggggc aagagggcgt
     2581 cccgcgccgt cgggaattcg aaagcgcgcc ccctctcctt tccccgtcgg agaaagcgat
     2641 ccgtccggca ggtctcctgg cttccggatc ttccgccgtc gggctccttc ccggacgcgc
     2701 gaccgtgtga accgatctca cgcgtccagt ggtttctcgc ccggggcgtc cccggtgaca
     2761 gtggcggggg ccgcgcggga tttacacccg cttccctcga ccgaacggag tcaaagaacg
     2821 agcggcgaag atacagcgcg gatatcatcc cgcgcaacca tgaccggcca ggactacacc
     2881 ctcgatttcg accgcctgcg ccgagtcgga ttccccgaag tcgtcctcgc cgaggggaag
     2941 accgcggcgc agatcgcgga gatctgccgg cagctcgcgc gcgcgcacga cgtcctcgtc
     3001 actcggctct tcccggcgca gtgggacgag ctgtccgtcc tgccgctccc cggccgcgcc
     3061 gactacgacg agcgctccgc gacgctccac ctctccgtcg gcaagcccat ggcgcgtctc
     3121 gaggggaacg tcgtcctcgt cacggccggc actgccgacg tgaccgtcgc ggaagagacg
     3181 cgccgcacgc tcgagtacct cggcgtcacg tcgaccctcg tgcccgacgt cggcgtcgcc
     3241 gggatcctgc gcctcctctc gcgcctggag caggtggagc aggccgacgt cgtcatcgcg
     3301 gtcgccggaa tggaagcgtc tctcttctcc gtcatcggag ggctcgtcgg ccggccgctc
     3361 atcgccgtcc cgacctcgcg tggatacggc gccgccttga gcggcgtctc gtcgctcctc
     3421 gccgcgttga actcgtgcgc caacggagtc accgcggtga acatcgactc gggctacgcg
     3481 gcggcgatgg ccgcgtaccg cattctttcg ggagggccgc gccgatggga tcgaggacga
     3541 aaagagggct ccgcgacgcc cgccagtaca tccacgaagc cgcgatcggc gtcgaccgca
     3601 agaggaagcg cgcgctcctc gggcggccga cgacgcccgt ccaggtaggt ccgaagacca
     3661 ccgtctcgga gctcctcgac tcctaccggg acgcatcgtt ccaggcccgc gcgctcggga
     3721 actgcgcggc gatcttctcg tcgatgctct ccgacaagga ccggccgacg atcttcctcg
     3781 gcctcgcggg atcgctcata gccgcgggga tgcggcaggt gatcacggac ctgatcgagc
     3841 agaacatggt ggacgtcgtc gtctcgaccg gcgcgatcat ctcgcaggac tattaccagg
     3901 tgcgcggcgg ccggcattac cacgggagcg tgaacgccaa cgacaaggag ctgcgcgacc
     3961 tctacatcga tcggctctac gacactttca tcgacgagga gaagtactgg aagaccgatc
     4021 tcgcggtgtc gcagttcgcc gacacgctcg cggggaagac gctctcctct cgcgcgttcc
     4081 tcgcgctgct cgccgaaagc gcgaagaaag accccggctc gattctcggt gcctgcgcgc
     4141 gcaacggcgt gccgctcttc gtcccggcgc tgaacgactc ctcgatcggc atcggcttga
     4201 ccgagcacta ccaccggatg cgcaaggacg gaaaacagcc gatgacgatc tcctccatcc
     4261 gcgacaacta cgagctcacg cagatcgttg tccggagtcc cgcgacctcc gcggtgtacg
     4321 tctccggcgg tgtgccgaag aactacatca acgactcgat cgtgatgtcg tacatcttcg
     4381 ggatcgacac cggcggccac cgttacgcga tccagatcac gaccgacgcg ccgcactggg
     4441 ggggcctggg aggttcgacg ctgtccgagg cgacgtcgtg gggcaaggtg tcgaagaagg
     4501 cgtcgcacga gatggcgttc gtcgagacgt cggtggcgct gccactcctg tacgggtacg
     4561 cgttgcagaa gcaggcgcac aagggacgga agcggctgcg gtacgcgtgg cgggaggaca
     4621 ttctcgagaa ggtgacgcgg gtctgatgac gcgcccctca ccctgaccct ctccccgcac
     4681 gcggggagag ggaaatagca aacgaaaaag ccccggtctc ccggggcttt cgatttccga
     4741 ttgccgattg ccgattgccg tcttagagca cttccccgcg cagcgggatc tcgatgaccg
     4801 cgcgctcctt gtcggaggtg aagagcttca ccgtcccgtc gagcgcgccc ttcgtcacgg
     4861 cgccgctcgc cttgacgacg acggtgtagg agacgccctc ctgggtcggc acgacgtccg
     4921 tcatgaaacc cgggatcgag acctcggcct tcgtgacctt gaccggcgtt cccggcttgt
     4981 tgttcgtcac gacgatgttg cgggtgatgg ggtccttgcc cgcggtgaag ttgccgaagt
     5041 tgaccgtgat cggcgaaacc gagacgcgcg gccggacgat gcccgagacc gggatctcga
     5101 gggtcggctg ctgctggacg ccggtcgtga cgcggaccgg tgcattgagg aggccttccg
     5161 gcgcgtcggc gagcaccgag acgttcagct tgtactggtc gcccggacgc ccggcgatct
     5221 tgtccttgtc gccggccggc gagatctcgg ccttgacgta cggctgggcg gtctcggcca
     5281 cggtcggctt gaagcccttc tcctcggaga tgaggatgac gtcctgcgaa gccgggtcgc
     5341 ccttgacgac cgagaagcgc acgtactgct ggggagccac gtcgacgaac ggctt
//
LOCUS       DASWAV010000321         2807 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U2665
            k141_18578, whole genome shotgun sequence.
ACCESSION   DASWAV010000321 DASWAV010000000
VERSION     DASWAV010000321.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960316
            Sequence Read Archive: SRR10260007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2807)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2807)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:47:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,929
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2807
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_18578"
                     /isolate="SMAG_U2665"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.36444 N 132.31889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(93..1112)
                     /gene="plsX"
                     /locus_tag="VF958_06590"
     CDS             complement(93..1112)
                     /gene="plsX"
                     /locus_tag="VF958_06590"
                     /EC_number="2.3.1.274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012647690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043811 - phosphate:acyl-[acyl carrier
                     protein] acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HEX9148814.1"
                     /translation="MRIAVDAMGGDQAPQVNVDAAVAAAREFEIPSLLVGRASRLAKI
                     LAESGYRGSDIEIVDAPEVVTMDDPATAAVRGKRNSSIRVAANCVRDGRAQGLVSAGH
                     TGAAMVSAKMVVGTIEGVDRPALAAVIPHLTGHCLLLDVGANPDAKTQHFKEFAVMGS
                     LYAQLAFGKESPSIGLMSIGEEDTKGTDRTKEAFKVLKETGLNFIGNVEGRDVFDGKV
                     DVIVTDGFTGNVILKVSEALGEMVEKLLREEIKKTLQASVGFLLSRSAFRSFKQRLDY
                     SEYGGAPLLGVKGCVIICHGRSSAKAIKNAIRFAAEFSRQRVAEKIQASIAELHAREA
                     SLLKA"
     gene            complement(1210..1383)
                     /gene="rpmF"
                     /locus_tag="VF958_06595"
     CDS             complement(1210..1383)
                     /gene="rpmF"
                     /locus_tag="VF958_06595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013788139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HEX9148815.1"
                     /translation="MPNPKRRHSKARRDKRRAHDALSAPALSECPNCHEPKAPHRVCA
                     RCGYYKGKQLINV"
     gene            complement(1390..1917)
                     /locus_tag="VF958_06600"
     CDS             complement(1390..1917)
                     /locus_tag="VF958_06600"
                     /inference="COORDINATES: protein motif:HMM:NF014657.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HEX9148816.1"
                     /translation="MKPAAALSLETITDDPLRFDFELPFTLEALDREPLVSISPARLV
                     GEVSRVEGGYALSARLTWSGQLECSRCLAPYDFANDEEFSLLLYPRAPVAEKELSLDR
                     DDFDAYFYDDAMLSVSPIAEERIQMAVPMKPLCREECRGLCPQCGSDLNVSECNCVSE
                     EIDPRWRALRLLKKE"
     gene            1982..>2807
                     /locus_tag="VF958_06605"
     CDS             1982..>2807
                     /locus_tag="VF958_06605"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX9148817.1"
                     /translation="MRVAVDARALLGQSTGIGTYTRGISLALAARDGIEVGLFTPRPI
                     DDPLSGNGAVSLHADRHRFGTVWVQTTLPGRAASWGADALLAALTIGPARGDTPLVSV
                     VHDLTAITHPEWHAARTLVGFLPLWESTVERAARFLCVSGATARELAGRYPEVRARIA
                     VARNGVDAEFSPFSDAAAAERTRRRFAKGRRYVLYLGTLEPRKDVETLVTACERLWTR
                     RRARPDLVLAGGAGWKSLALLRRIARSAFRDKIHVAGYASRSMAVDLYRAAEAFAYP"
BASE COUNT          439 a          935 c          930 g          503 t
ORIGIN      
        1 tcgacgagct tctcgaggtc ggcgttggtc agaaccttct tgggtacggc agctccggtg
       61 ccggcgacgc gcgctcgctt catgactcga tcttaagctt tgaggagcga agcttcccgc
      121 gcgtgcagct cggcaatcga cgcctggatc ttttcggcga cgcgttgccg ggagaactca
      181 gcggcgaagc ggatcgcgtt cttgatcgcc ttggcgctcg atcgcccgtg acagatgatc
      241 acgcacccct tgacgccgag gaggggcgcg ccgccgtact cggagtagtc gagccgctgc
      301 ttgaagctcc ggaaggcgct ccgggaaagc aggaatccca ccgacgcctg gagagtcttc
      361 ttgatctctt cgcgcaggag cttctccacc atttctccga gggcttcgga caccttgaga
      421 atcacgtttc cggtgaagcc gtcggtgacg atgacgtcga ccttgccgtc gaacacgtcg
      481 cggccctcga cgtttccgat gaagttcagg ccggtctcct tcaggacctt gaatgcctcc
      541 ttcgtccggt cggtcccctt cgtgtcttcc tcgccgatag acatcaggcc gatcgagggg
      601 gactcttttc cgaacgcgag ctgggcatag agcgagccca tgaccgcgaa ttccttgaag
      661 tgctgcgtct tcgcgtccgg gttcgccccg acgtccaaga gcaggcagtg cccggtgagg
      721 tgcgggatca ccgccgcgag tgcggggcga tcgactccct cgatggtccc gacgaccatc
      781 ttggcggaga ccatggcggc gcccgtatgc cccgccgaga cgagcccctg cgccctcccg
      841 tcgcgcacgc agttggcggc gacgcgaatc gaggagttgc gctttccccg cacggcggcc
      901 gtcgccgggt cgtccatcgt cacgacttcg ggcgcgtcca cgatctcgat gtcggagccg
      961 cgataaccgg attccgccag gattttcgcg agccgcgatg ccctgccgac gaggaggctc
     1021 gggatctcga actcgcgagc cgcggcgact gccgcgtcca cgttgacctg gggagcctga
     1081 tctcccccca tcgcatcgac ggcaatgcgc atctaaccga gttgtctcat cgggaacacg
     1141 gcgtccggat tatagcgacc gctacgcggg aaccgcatgc ttcgattccc gatttccgat
     1201 tcctgccggt caaacgttga tgagctgctt gcccttgtag taaccgcacc gggcgcagac
     1261 acggtgcggc gctttcggct cgtgacagtt gggacactcc gacagcgcgg gagccgaaag
     1321 ggcgtcgtgc gcgcggcgct tgtcgcggcg ggccttgctg tggcggcgtt tcggattcgg
     1381 catgggttcc tactctttct tcaaaagccg gagcgcgcgc caccgcgggt cgatctcttc
     1441 ggacacgcag ttgcactccg agacgttcaa gtcgctgccg cattgcgggc agagcccgcg
     1501 gcactcctcg cggcaaaggg gtttcatcgg gacggccatc tggatccgct cctcggcgat
     1561 cggcgagacg gaaagcatcg cgtcgtcgta gaagtaagcg tcgaagtcgt cgcgatccag
     1621 ggagagctcc ttctcggcga ccggagcccg cgggtagaga aggagagaga actcctcgtc
     1681 gttggcgaag tcatagggcg cgagacaccg actgcactcg agctgaccgc tccacgtgag
     1741 tcgcgccgac agggcgtagc cgccttcgac gcgcgagacc tctccgacga gacgcgccgg
     1801 cgagatcgag acgagaggct cccggtcgag cgcctcgagc gtgaagggca gctcgaagtc
     1861 gaaccgaagg gggtcgtcgg taatggtctc gagagagaga gcggcagcgg gtttcatggt
     1921 ggaatcggcg attttaccga ttccggttcc ccagtcaaac gtctgatacc ttgagcgttt
     1981 gatgagagtt gcggtcgacg cgcgcgctct cctcggtcag agcacgggca tcggcaccta
     2041 cacccgcggg atctctctcg ctctcgccgc ccgtgacggc atcgaggtgg ggctcttcac
     2101 gccccggccg atcgatgacc cactttcggg caacggggcg gtgagcctcc acgccgaccg
     2161 acaccgcttc ggaacggtct gggtgcagac cacgcttccg ggccgggccg catcgtgggg
     2221 ggcggacgcg ctcctcgcgg cattgacgat cgggccggcc cgcggagata cgccgctcgt
     2281 ctcggtcgtc cacgatctga ccgcgatcac ccatcccgag tggcacgcgg cccggacgct
     2341 cgtcgggttc ctgccgctct gggagagcac ggtcgagcgg gccgcgcggt tcctctgcgt
     2401 ctcgggggcg actgcacgcg agctcgccgg acgctacccg gaggtgcgcg cccggatcgc
     2461 tgtcgcgcgc aacggcgtgg acgccgagtt ctctcctttt tccgacgccg ctgccgccga
     2521 gcggactcgc cggcgtttcg cgaaggggcg gcgctacgtc ctctacctcg gaacgctcga
     2581 gccgcgaaaa gacgtcgaaa cgctcgtcac cgcctgcgag cggctgtgga cccgccggcg
     2641 ggcacgaccg gatctcgtgc tggccggcgg cgccggctgg aagtccctcg ccctcctccg
     2701 ccgcatcgca cggtcggcct tccgggacaa gatccacgtg gcgggatacg cgagccgctc
     2761 catggccgtg gacctctacc gggctgcaga ggcgttcgcc tatccgt
//
LOCUS       DASWAV010000322         2413 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U2665
            k141_256942, whole genome shotgun sequence.
ACCESSION   DASWAV010000322 DASWAV010000000
VERSION     DASWAV010000322.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960316
            Sequence Read Archive: SRR10260007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2413)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2413)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:47:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,929
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2413
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_256942"
                     /isolate="SMAG_U2665"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.36444 N 132.31889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..655)
                     /locus_tag="VF958_06610"
     CDS             complement(<1..655)
                     /locus_tag="VF958_06610"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HEX9148818.1"
                     /translation="MIRTLYRGIFLGLLGASFAVGAGPVETEAYSSHLMVDQEVTVSA
                     RITGVIEKINVDRGSVVKRGQSLAQLELQEFEQGVREAKEDLELRKVELARAQALSTG
                     NIMSKAELDERRARHAVALAAWEKARAIRDYAFIRAPFPGVVTEKYARVGQKVIDNQN
                     VPLFKITAFEPLLARVYLPEQRLMSVRRGDRVEVVPDNFPNARTTGVVQYISPTVDAA
                     "
     gene            701..1045
                     /locus_tag="VF958_06615"
     CDS             701..1045
                     /locus_tag="VF958_06615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9148819.1"
                     /translation="MSIALADEKPDIQHKGPRANFSWRIVEPWDERRLAAFLMVKHRR
                     NESDWTRRPSPFFASLGRSVVECSIPLTATDSVGEIEKLLTQELRGILAGADRVYRDI
                     LKSPDWRQEPPP"
     gene            1210..2340
                     /locus_tag="VF958_06620"
     CDS             1210..2340
                     /locus_tag="VF958_06620"
                     /inference="COORDINATES: protein motif:HMM:TIGR03187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DGQHR domain-containing protein"
                     /protein_id="HEX9148820.1"
                     /translation="MLSVPSLRLNQFGVSFYEALLSGADLQSLVGFEVLSFGLAGQTT
                     RGHARQTKGARVNWELLEKRIAASEHAYQRPIIRKKIDELVAYYLQCADSGTLPAIPG
                     SVLLVSDRRLEFSPSGGRGDAGLLKLPEESGILRALDGQHRLLALHQLAEKHNLKDFQ
                     VPAVVFDNLTADQVVELFVTINSKHTKLNPSHLISLSGRRLYRDENLAAAHDVIRTLN
                     EDDGSPLRGQIKVLGIGRGSVTQASLADELRDVFAALDAAGGRTAREFRENSRRFFLN
                     YFKAAARAFPTAFHGRKYSVKSSTALRAFIRIVPYVISMIRKRGGDVWSAGELDAALR
                     PWSESIGDRRFETDTVWRDKMTAGKRSAVEILARELRQGLNA"
BASE COUNT          412 a          806 c          771 g          424 t
ORIGIN      
        1 tcgcggcgtc gacggtcggg ctgatgtatt gcacgacccc ggtggtacgc gcgttgggga
       61 agttgtcggg aaccacctcg acccggtctc cgcgccggac gctcatgagc cgctgctcgg
      121 ggaggtagac gcgcgcgaga aggggctcga aggcggtgat cttgaagagg gggacgttct
      181 ggttatcgat cactttttgt cccacgcgcg cgtacttctc ggtcacgacg ccgggaaacg
      241 gtgctcggat gaacgcatag tcgcggatcg cccgcgcctt ctcccaggcg gcgagcgcga
      301 cggcgtgacg ggcgcgcctt tcgtccagct ccgccttgct catgatgttt cccgtcgaaa
      361 gcgcctgagc ccgcgcgagc tcgaccttgc ggagctccag gtcctctttc gcctcgcgga
      421 ccccctgctc gaactcctgg agctcgagct gggcgagaga ctggcccctc ttgacgaccg
      481 acccccggtc cacgttgatc ttctcgatca cgcccgtgat ccgggcactc accgtgactt
      541 cctgatccac catgaggtgg gaggagtacg cctctgtctc caccgggccg gcgccaacgg
      601 caaagctggc gccgagcagg ccgaggaaga ttccgcggta aagcgttcgg atcatgtctg
      661 gcggtcgaat cgcgccaaat gctatcctca aagcagcttc atgtcgatcg ctctcgccga
      721 cgagaaaccc gacatccagc acaagggtcc ccgagcgaac ttcagctggc ggatcgtgga
      781 gccgtgggac gaacggcgcc tggccgcctt cctgatggtg aagcatcgcc ggaacgaatc
      841 ggattggacc cggcgtccct cccccttttt cgcttcgctc ggccgttccg tcgtcgagtg
      901 ctcgattccg ctgaccgcga cggattcggt cggggaaatc gagaagctct tgacgcagga
      961 actgcgaggg attctcgcgg gcgccgaccg tgtctatcgc gacatcctga agagcccgga
     1021 ctggcggcag gagccgccgc cgtagccgcg ctcgagagac cgacgatcac gggcgaatga
     1081 ccccccggcg gccccgaaac gaatttgcat ataatccgcg ccacggacgc gtggagtcca
     1141 tcacgggagc ggccgttcca agctgttccg ccggcgtgac ttaggacgac ggcggcagag
     1201 gaggtaagga tgctctcggt tccttctctc cggctcaatc aattcggcgt gagtttctac
     1261 gaagccctcc tctcgggggc ggacctgcag tcgctcgtcg gcttcgaggt cctctccttc
     1321 ggcctggccg gccagacgac ccgcggacac gcgcgacaga cgaagggggc gcgggtcaac
     1381 tgggagcttc tcgagaagcg tatcgcggcg tccgagcacg cctatcagcg gccgatcatc
     1441 cgcaagaaga tcgacgagct cgtcgcgtac tacctgcagt gcgcggattc cggcacgttg
     1501 ccggccattc cgggaagcgt tcttctcgtc tctgaccggc ggctcgaatt ctctccctcc
     1561 gggggccgcg gcgacgccgg cctcttgaag cttccggagg aatccggaat cctccgcgcg
     1621 ctcgacgggc agcaccggct cctggcgctc caccagctcg ccgaaaagca caacctgaag
     1681 gacttccagg tgcccgcggt cgtcttcgac aacctgaccg ccgaccaggt cgtggagctc
     1741 ttcgtcacca tcaacagcaa gcacacgaag ttgaaccctt cgcacctgat ctcgctctcc
     1801 gggcggcgcc tctaccggga cgaaaacctc gccgcggctc acgacgtgat ccggactttg
     1861 aacgaggacg acggatcgcc cctgcgcgga cagatcaaag ttctcggcat cggccgcgga
     1921 agcgtgaccc aggcctccct ggccgacgag ctgcgcgacg tcttcgccgc gctggatgcc
     1981 gccggcggcc ggacggcccg ggagttccgg gaaaactcgc gccggttttt cctgaactac
     2041 ttcaaggcgg ccgctcgcgc gtttccgacc gcgttccacg ggcgtaagta ctcggtcaag
     2101 agttcgaccg cgctgcgcgc cttcattcga atcgtgccgt acgtcatttc catgatccgc
     2161 aaacgtggcg gcgacgtctg gtccgccggg gagctcgacg ccgcgctccg tccctggtcc
     2221 gagtcgatcg gcgacaggcg cttcgagacc gacacggtct ggcgcgataa gatgaccgct
     2281 ggcaagcgct ccgccgtcga gattctcgcg cgcgagcttc ggcaggggct caatgcctag
     2341 cgaaacgcgg ggagcggaca cgtggagtca aacctcggcg cggcggaatc gtagatagag
     2401 gtacaagcta tgg
//
LOCUS       DASWAV010000323         3727 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U2665
            k141_497878, whole genome shotgun sequence.
ACCESSION   DASWAV010000323 DASWAV010000000
VERSION     DASWAV010000323.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960316
            Sequence Read Archive: SRR10260007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 3727)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3727)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:47:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,929
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3727
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_497878"
                     /isolate="SMAG_U2665"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.36444 N 132.31889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..201
                     /locus_tag="VF958_06625"
     CDS             <1..201
                     /locus_tag="VF958_06625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015743101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylpyrophosphate synthetase"
                     /protein_id="HEX9148821.1"
                     /translation="VHPVFSGPARQRIEASKLETLFVTNTIPLDDSMKDCRKIHTLSV
                     APLLGEAIQRIHQGASVSSLFV"
     gene            271..963
                     /locus_tag="VF958_06630"
     CDS             271..963
                     /locus_tag="VF958_06630"
                     /inference="COORDINATES: protein motif:HMM:TIGR00731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0008097 - 5S rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25"
                     /protein_id="HEX9148822.1"
                     /translation="MKTIELTVERRETRGKNSARRTRAGGTVPAVLYGAGPDTVPITV
                     NLRALSDAFRGGAGENAIFLLKLHGSDQTRHAMIRDLQRDPLSRRLMHLDFVRVLMDA
                     KIRVRVPVEVVGIAKGVKTEGGILDFVTREIEIECLPGNIPEHLPIDVTELGIGDALR
                     VSDVKAPEGVEVVDDAEKVVLHVAHPTHEEVAAAPEAVEAAAEPAEPEVLRKGKAVAE
                     EEGEEKKEKKEK"
     gene            979..1593
                     /gene="pth"
                     /locus_tag="VF958_06635"
     CDS             979..1593
                     /gene="pth"
                     /locus_tag="VF958_06635"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011343144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HEX9148823.1"
                     /translation="MGLGNPGDRYENTRHNAGFQAVEKLAAEEGIRIRSREGKSRVGR
                     GTIAGESVVLALPQTYMNASGEAVRVLCEQNGIGPEDLCVVFDDIDLPLGVLRMRARG
                     SAGGQRGMRSVIDRLGTTEFARLRVGVRGERYSREKDELGDYVLAPFARGEREIFRKS
                     IERAVEALRVWLDEGIDAAMRLANQKPSSPGSDESGLTSTRRNE"
     gene            1590..2015
                     /gene="rpsF"
                     /locus_tag="VF958_06640"
     CDS             1590..2015
                     /gene="rpsF"
                     /locus_tag="VF958_06640"
                     /inference="COORDINATES: protein motif:HMM:NF013419.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HEX9148824.1"
                     /translation="MRLYDIVALVTPDLNDEDAQKVAAEYRKILSDGGAEIVKDEPWG
                     RRRLAFPILRKREAYYHYFQVSAEPALVAETERRMKLSDQVLRHLAVRADVELKRAAK
                     FAAKQQAKAARRPPRPPAPMPAVASPQEAPPIAEKGEPS"
     gene            2012..2302
                     /gene="rpsR"
                     /locus_tag="VF958_06645"
     CDS             2012..2302
                     /gene="rpsR"
                     /locus_tag="VF958_06645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006314893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HEX9148825.1"
                     /translation="MSPAPRGGTRSATASRDGKTAGRKKFFVRRRKVCKFCAEKIEYV
                     DWKDIKLLQSFIPERGKILPRRISGTCALHQRKLQTAIKRARSIALIPYATD"
     gene            2391..3380
                     /locus_tag="VF958_06650"
     CDS             2391..3380
                     /locus_tag="VF958_06650"
                     /inference="COORDINATES: protein motif:HMM:NF021506.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2232 domain-containing protein"
                     /protein_id="HEX9148826.1"
                     /translation="MEANRLEQPAADRPHAAAREIAFAAGFSAISFALFLILPLVGAI
                     GLPLSAVSTVRLAHRRGAVAAMIASALAGSLVAGVALAAGDAGGWSRGLIAAGTAALP
                     AIFAALARRSGKPSRAYLLLCAAGVAALAVLFLGGAAASGRPVGDEIAKEFDRMIPAA
                     LESYTRSGMDSETVVRVRATLETAREFSRRYWAGLLGLLWVFSAAISFYLGARSARPA
                     PSAEGARFEDLRVPAAVAPLFVAAGAGSVLLQEGARPLAGSVLLPLSALYFVAGLSII
                     CHFARKWFRFWLLRVGLYTLVAYFPMNVGVALLGLFDWYVDFRRRGAGAIEKR"
     gene            3377..>3727
                     /gene="rplI"
                     /locus_tag="VF958_06655"
     CDS             3377..>3727
                     /gene="rplI"
                     /locus_tag="VF958_06655"
                     /inference="COORDINATES: protein motif:HMM:TIGR00158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HEX9148827.1"
                     /translation="MKVILADDVRGLGHRGETVTVKPGFARNYLFPQGLAWESTTANM
                     RRLSEEKKKYDEKSLHEKTAAEDVAKRVAGLTVTVSKKAGEGDVLYGSVTPADIADAL
                     ATRGVEVDRRRIELA"
BASE COUNT          625 a         1200 c         1290 g          612 t
ORIGIN      
        1 gtgcaccccg tgttttccgg gccggcgcgc cagcgaatcg aggcctcgaa gctcgagacc
       61 ctcttcgtca cgaacacgat cccgctcgac gactcgatga aggattgccg caagatccac
      121 accctgtccg tcgcccccct cctgggcgag gcgatccagc gcattcacca gggcgcttcc
      181 gtttcttccc tgtttgtgta gaatgtcgcg tttttccgca ggctgccgac ggcggccggg
      241 agcgggcccg ctttccacga ggggaagaag atgaagacca tcgagctcac ggtcgaacgg
      301 cgggagaccc gggggaagaa ctccgcccgc cggacgcgcg ccggaggaac ggtcccggcg
      361 gtcctttacg gggccgggcc ggacaccgtg cccatcacgg tgaaccttcg cgccctttcg
      421 gacgccttcc gcggcggagc aggggagaac gcgatctttc tcctcaagct ccacggttcc
      481 gaccagaccc gccacgcgat gatccgggat ctgcagcgcg accctctctc ccgccgcctc
      541 atgcacctcg acttcgtgcg ggtcctgatg gacgcgaaga tccgggtccg ggtcccggtc
      601 gaagtcgttg gcatcgccaa gggcgtcaag acggaagggg gcatcctcga cttcgtgact
      661 cgagagatcg agattgagtg cctccccggc aacatcccgg agcaccttcc gatcgacgtg
      721 acggagctcg gaatcggcga cgcccttcgc gtctccgacg tgaaagcgcc cgaaggggtc
      781 gaagtcgtcg acgacgccga gaaagtcgtg ctccacgtgg ctcacccgac ccacgaggaa
      841 gtggccgcgg ctcccgaggc cgtcgaagcc gctgccgagc cggccgagcc ggaggtcctg
      901 cgcaagggca aggccgtcgc cgaggaagag ggggaagaga agaaggagaa gaaggagaag
      961 taacgcctgc acgcgatcgt ggggctcggc aacccgggcg accgctacga gaacacccgc
     1021 cacaatgcgg gattccaggc ggtcgagaag ctggcggcgg aagagggcat ccggattcga
     1081 tcgcgcgagg ggaagtcccg ggtcggtcga ggaaccattg cgggagagag cgtcgtgctc
     1141 gcgcttccgc aaacgtacat gaacgcctcc ggagaggccg tccgagttct ttgcgaacag
     1201 aatggcatcg gtcctgaaga cctctgtgtc gtcttcgacg acatcgacct gcccctcgga
     1261 gtcctgcgga tgcgagcccg cggctcggcc ggcgggcagc gtggaatgcg ttcggtcatc
     1321 gatcgtctcg ggacgacaga gttcgcccgg ctgcgcgtcg gggtgcgggg cgagcgctac
     1381 tcgcgcgaga aggacgagct cggggattac gtcctcgcac cgttcgctcg gggggagagg
     1441 gagatcttcc gcaagagcat cgaacgcgcg gtcgaggcgc tccgggtctg gctcgacgaa
     1501 ggaatcgatg ccgcaatgag gctcgcgaat cagaaaccat cctctcccgg ctccgatgag
     1561 tcgggattga catccacgag gaggaacgaa tgaggcttta cgacatcgtc gctctggtca
     1621 cccccgacct gaacgatgaa gacgcccaga aagtggcggc cgagtatcga aagattcttt
     1681 cggacggtgg cgccgaaatc gtcaaggacg agccgtgggg acggcgccgg ctggctttcc
     1741 cgatccttcg caagcgggag gcctactacc actacttcca ggtatctgcc gagccggcgc
     1801 tcgtggcgga gaccgagagg cggatgaaac tctccgacca ggtccttcga cacctggccg
     1861 ttcgcgccga tgtggagctg aagcgcgcgg cgaagttcgc cgcgaagcag caggcgaaag
     1921 cggctcgacg tccgccgcgt ccgcccgcgc cgatgccggc cgtggcgtcg ccgcaggaag
     1981 ctccgccgat cgccgagaag ggagagccgt catgagtcct gcgccgcgag gaggcacgcg
     2041 ttccgccacg gcgagccgcg atggaaagac ggcgggccgg aagaagttct tcgtccgacg
     2101 ccgcaaggtc tgcaagttct gcgcagagaa gatcgagtac gtcgactgga aggacatcaa
     2161 gctccttcag tcgttcattc cggagcgcgg gaagatcctg ccccgacgca tctcggggac
     2221 gtgcgcgttg caccagcgca agttgcagac ggccatcaag cgggcccgtt cgatcgcact
     2281 gattccgtac gcgacggact agccgaaacg ggaaacggga aacgggaaaa gaggaaggcg
     2341 actccgtcgt ttttcccttt tccgttttcc gtttcccggc ctcaccactg atggaggcca
     2401 accgcctcga acagccggcc gccgatcgcc cgcacgcggc ggcccgggag atcgcgttcg
     2461 ccgccggttt ctcggcgatt tcgttcgcgt tgttcctgat cctgccgttg gtgggcgcga
     2521 tcggcctgcc gctttcggca gtgtcgaccg tccggctcgc ccaccgtcgc ggagccgtgg
     2581 cggcgatgat cgcgtcggcg ctcgccggct cgctcgtcgc cggggtcgcg ctcgccgctg
     2641 gagacgcggg cggttggtcg cgaggcttga tcgccgcggg gacggcggcc cttccggcga
     2701 tcttcgcagc gctcgcgagg aggagcggaa agccgtcccg cgcctacctg cttctctgcg
     2761 ccgccggagt cgccgcgctg gcagtcctct tcctcggcgg agcggccgcc tcggggcggc
     2821 ccgtgggaga cgagatcgcg aaggaattcg accggatgat cccggcggcg ctcgagtctt
     2881 acacgcgatc cgggatggac agcgaaaccg tcgtccgcgt gcgggcgacg ctcgagaccg
     2941 cgcgggagtt cagccggcgg tactgggccg gtctgctcgg gctgctgtgg gtcttctcgg
     3001 cggcgatctc gttctacctg ggcgcgcgct cggcgcgacc ggccccctca gccgagggcg
     3061 cccggttcga ggatctgcgc gtgcccgctg cggtggcgcc gctcttcgta gcggcgggcg
     3121 cgggatcggt gctcctccag gagggggccc gcccgcttgc tgggagcgtc ctgctgccgc
     3181 tctccgcctt gtatttcgtc gccgggctct ctatcatctg ccacttcgcc cggaagtggt
     3241 tccgtttctg gttactccgc gtcggtctct acactctggt cgcgtacttc ccgatgaacg
     3301 tgggggtcgc gctgctgggg ctgttcgact ggtacgtgga ttttcggcgc aggggcgcgg
     3361 gagcaatcga aaagcgatga aagtcattct ggccgacgac gttcgcgggc tcggacaccg
     3421 aggcgaaacg gtgacggtga agcccggttt cgcccgcaat tacctgttcc cgcagggtct
     3481 cgcctgggag tcgaccacgg caaatatgcg acggctttcc gaggagaaga agaagtacga
     3541 tgagaagtcg ctccacgaga aaaccgcggc cgaggatgtc gcgaagcgcg tggcgggatt
     3601 gaccgtgacc gtctcgaaga aggcggggga gggcgatgtg ctttacggct ccgtcacgcc
     3661 agccgatatc gccgatgccc tcgcgacccg gggggtcgag gtcgaccgcc gccgcatcga
     3721 gctcgcc
//
LOCUS       DASWAV010000324         1696 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U2665
            k141_574748, whole genome shotgun sequence.
ACCESSION   DASWAV010000324 DASWAV010000000
VERSION     DASWAV010000324.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960316
            Sequence Read Archive: SRR10260007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 1696)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1696)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:47:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,929
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1696
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_574748"
                     /isolate="SMAG_U2665"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.36444 N 132.31889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            247..>1696
                     /locus_tag="VF958_06660"
     CDS             247..>1696
                     /locus_tag="VF958_06660"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013496601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004832 - valine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006438 - valyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HEX9148828.1"
                     /translation="MAEEPSARLEKTFDPAHFEERWYRTWEAEGRFQPVGPPGSPRFV
                     MVIPPPNVTGRLHIGHAFGRTLEDVLARWKRMLGYRVLWVPGTDHAGIATQMVIEREL
                     AKQGIDKRDLGRQKFVERVWAWKRDAKDTIQSQIRRLGCSLDWTRERFTLDPDLSAAV
                     RHAFVRLYRDGLIYRGRYVVNWCPRCGTAVSDLEVVHEEVEGSLYRIRYDVPGVASGA
                     VVATTRPETMLGDTALAIHPDDPRTAKLRGRTAILPIVGRELPIIEDPVLVDREFGTG
                     IVKVTPAHDANDFATAERHALAPVVVIGPDAKMTAEAGEYAGLDRLEARRKVIERLQS
                     ENRLVGIDPHRYALGHCQRCDTVIEPYLSTQWFVKIQPLAEPAIRAVEAGDVRFVPEL
                     WTKTYFEWMRNIHDWCISRQLWWGHRIPAFTCANGHLTVSEEDPSACPTCGSAELTQD
                     PDVLDTWFSSQLWPFSVLGWPKETEDMREFYPT"
BASE COUNT          288 a          557 c          587 g          264 t
ORIGIN      
        1 ggcgaagctc gtagagaagc tttccgaccg catcgtccgc gaagtcgcgt gggaagtcgt
       61 tcccgaggcc gcggagctcg ccgtgcgcga gcgcctgcgg gagctcgaaa gcggcgtcga
      121 gtagaacctc ccaggcgccg cggcggcaga atagcgattc cgcctcgttt ctgataagca
      181 acgccccttc gcatgtccgg agagaacccg caggcgcgtc cggaaggaac cggatcggtg
      241 gaaaccgtgg ctgaagaacc atcggcgcgc ctggagaaga ccttcgaccc ggcgcacttc
      301 gaggagcgct ggtatcggac gtgggaagcg gaagggcgtt tccagccggt cggccctccc
      361 ggctcgcccc gtttcgtcat ggtcatcccg cccccgaacg tcacggggcg gctgcacatc
      421 ggccatgcgt tcgggcggac tctcgaggac gttctcgccc gctggaagag gatgctcggt
      481 taccgcgtcc tgtgggttcc cggaacggac cacgccggaa tcgcgacgca aatggtgatc
      541 gagcgggagc tggcgaagca ggggatcgac aagcgcgacc tcggacggca gaaattcgtc
      601 gagcgggtgt gggcctggaa gagggacgcg aaggacacga tccagtctca gatccgccgg
      661 ctcgggtgct cgctcgactg gacccgcgaa cgcttcacgc tcgacccgga tctttcggcg
      721 gcggtgcgtc acgccttcgt ccggctttac cgcgacgggc tcatctaccg cggccgttac
      781 gtcgtcaact ggtgcccgag gtgcgggacg gcggtctcgg acctcgaggt cgtgcacgag
      841 gaagtcgagg gctcgctcta ccggatccgc tacgacgtcc cgggggtggc ctccggagcc
      901 gtcgtggcaa cgacgcggcc ggaaacaatg ctgggggaca ccgcgctcgc gatccacccg
      961 gacgacccgc gcaccgcgaa gctccgcgga cggaccgcaa ttctcccgat cgtcgggcga
     1021 gagctcccca tcatcgagga tccggtcctc gtcgaccgcg agttcggcac cggcatcgtg
     1081 aaggtcacgc cggctcacga cgcgaacgat ttcgcgacgg ccgagcggca cgcgctcgcc
     1141 ccggtcgtcg tgatcggtcc cgacgcgaag atgacggcgg aagcagggga atacgccggt
     1201 ctcgaccggc tcgaggcccg gaggaaggtg atcgagcggc ttcagtcgga gaatcgcctc
     1261 gtcgggatcg acccgcaccg gtacgctctc gggcactgcc agcgctgcga cacggtgatc
     1321 gaaccgtacc tttccacgca gtggttcgtg aagatccagc ctctggcaga gccggcgatc
     1381 cgcgccgtgg aggcgggcga cgtgcggttc gtgccggagc tctggacgaa gacctacttc
     1441 gagtggatgc gcaacattca cgactggtgc atctctcggc agctctggtg gggacaccgg
     1501 atcccggcat tcacgtgcgc caacggccac ctgaccgttt cggaagagga tccctcggcg
     1561 tgcccgacct gcggctccgc cgagctcacg caggacccgg acgtgctcga cacgtggttc
     1621 tcctcccagc tctggccgtt ctccgtgctg gggtggccga aggagacgga ggacatgcgg
     1681 gagttctacc cgaccg
//
LOCUS       DASWAV010000325         5588 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U2665
            k141_458115, whole genome shotgun sequence.
ACCESSION   DASWAV010000325 DASWAV010000000
VERSION     DASWAV010000325.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960316
            Sequence Read Archive: SRR10260007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 5588)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5588)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:47:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,929
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5588
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_458115"
                     /isolate="SMAG_U2665"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.36444 N 132.31889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2588
                     /locus_tag="VF958_06665"
     CDS             <1..2588
                     /locus_tag="VF958_06665"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HEX9148829.1"
                     /translation="GGMGEVYKAKDTRLGRDVAVKVLPAHMSANAELRQRLDREAKTI
                     SSLSHAHICALYDVGREGDRDYLVMEYLEGESLAARLDKGALPSEQLLRYGIEIADAL
                     DKAHRQGIVHRDLKPGNVMLTKSGVKLLDFGLAKFQATARGESLSGASRLATQASSPL
                     TERGTVLGTFQYMAPEQLEGGEADARSDIFAFGAVLYEMATGKKAFTGKSQASLAGSI
                     LRDDPPSVNEVAPMTPPALNRVIKTCLAKDPEDRFQTAHDVKLQLQWIAEGGSQAGLP
                     APVAARRKSRERLAWGVAAAALVAAAALGFGFVRRAPAPRRSVRFEVAIPEGVVAIDA
                     PRISPDGRYLAFNATDAEGKTRIWLRPLNALAAQPLPGTDGTTRPFWSPDSRFLGFFA
                     EGKLKKIEVTGGPAQKICDAPTGADGSWSPDGVILFDGTGNDPINRVSAAGGTPTAAV
                     KADKDVQVGWPEFLPDGRHFLYMSIGTKQEDSAYRVGSLDSTETKRFAPAQTLVTYAP
                     PGYLLFVRDRTLVAQQFDAKAVKTKGEPIPLAEQIGTDNVGLATFSVSREGTLAYRTG
                     ETGNRLLWMDRSGKELDPVGDHGQYSNPVLSPAGDRLAFGLNDPRNGNNDIWIRDLAR
                     GVSSRFTFGVGANNGVPIWSPDGNTIVFRSDREGTLNLYEKAASGQGEEKILLKTDEI
                     KFATDWSRDGRYIAFSSQNTKTGWDSWALPTFGDRKPFPIVAGQFAELNPMFSPDTRF
                     VAYQSNESGNAEIYIQTFPVASGKWQVSTGGGTDPSWRADGKELYYRSADQKLMAVEI
                     QAGETLKAGIPQPLFPARVRTGTARNKYLATKDGQRFLLVAPLGREALIPTTVVLNWN
                     ADLGR"
     gene            2654..3703
                     /locus_tag="VF958_06670"
     CDS             2654..3703
                     /locus_tag="VF958_06670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011403683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin-independent methionine synthase II
                     family protein"
                     /protein_id="HEX9148830.1"
                     /translation="MTLPTEPVGSIPRPPELIEGLKGSLPEDRMDSLFDAAVRDTIRR
                     FEETGSTVITDGEQRKPSFATYPIHGAKNIGPGGVTIPFKDGHTRQLPVLTAGPFRYA
                     TYASSYLEAAGKYARTPLKQAVISASALSLLYPQAGIPGYTREEFLDDLVREAQTDIR
                     RCLDAGATVQIDFTEGRLAVKLDPSKNLLNSFVDLNNRVLAAFSAEERRRIGVHTCPG
                     GDQDSTHSADVDYAELLPALFRLNVGNFYVQLSSEKDRPRVLRILGEHARGDRRIFVG
                     VTDPIDPRIETADEVRDRVLEAAKFIDPGHLGTTDDCGFSPFGDDTSTSRDTAFAKIR
                     ARVEGTQMASRALGV"
     gene            complement(3710..4177)
                     /locus_tag="VF958_06675"
     CDS             complement(3710..4177)
                     /locus_tag="VF958_06675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015159494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RES domain-containing protein"
                     /protein_id="HEX9148831.1"
                     /translation="MLVWRIASRRHSALDGEGARAAGGRWNSKGIPMVYTSATLSLAA
                     LEYLVNVGLEEAPADLVSISVEVPATVSRRELGIGELPASWRTYPAPERLAAIGTNWA
                     SSLESAVLIVPSCVIPEEKNWLVNPRHPDARRIRIEKKAAFRFDPRLRRRKAR"
     gene            complement(4178..4627)
                     /locus_tag="VF958_06680"
     CDS             complement(4178..4627)
                     /locus_tag="VF958_06680"
                     /inference="COORDINATES: protein motif:HMM:TIGR02293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin Xre/MbcA/ParS toxin-binding
                     domain-containing protein"
                     /protein_id="HEX9148832.1"
                     /translation="MARRSAVTARAIAETLGGPRALRGSVGTFDDLRRRVEEGLRYES
                     LDALSSNLEIGLAAMRAILRLPERTLARRKNERRLRPDESDRLMRIGRVATLAEVTLG
                     SREKAASWLKKKNRALGDRAPLDRLDTDLGSREVEDLLLRIAHGVYS"
     gene            4699..>5588
                     /locus_tag="VF958_06685"
     CDS             4699..>5588
                     /locus_tag="VF958_06685"
                     /inference="COORDINATES: protein motif:HMM:NF024910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HEX9148833.1"
                     /translation="MSDATPGDTLSRRPLGFWSAAALVVGHTIGVGVFLTPVELIGAL
                     GSPAWTLGLWLTCGALVVCGAFTFGELVSRFPQTGGLYVYLREAFGGRAAFLYGWLCL
                     LVVDPGVTAALAAGVSPYAAVLWPAMEGREKVFAVGLVWALALVNMAGLRPSARFFTG
                     LTLVKILALAGVVVLAFAAGSGSWSHFVPFAPRHPGAPPIGEALGLALVGAFFSFGGF
                     WEASRVAGETREPGRTLPRALALGVATVTLAYVATTLAFVYLVPVEGMTSPVEFARRA
                     GEAMLGHAGPSAFAAVVVLSV"
BASE COUNT          883 a         1912 c         1868 g          925 t
ORIGIN      
        1 cgggcgggat gggcgaggtc tacaaggcga aggacacgcg gctgggccgg gacgtggccg
       61 tcaaagttct tccggcgcac atgtcggcga acgccgagtt gcgccagcgg ctcgaccgcg
      121 aggccaagac gatctcgtcg ctctcgcatg cccacatctg tgcgctctat gacgtcgggc
      181 gcgaggggga ccgcgactac ctggtcatgg agtacctcga gggtgagagc ctggccgcgc
      241 ggctggacaa gggcgcgctc ccctccgaac agctccttcg ctacggcatc gagatcgctg
      301 acgccctgga caaggcgcac cggcagggaa tcgtgcaccg ggacctgaag cccggcaacg
      361 tcatgctcac gaagtccggg gtcaagctcc tcgatttcgg cctcgccaag ttccaggcga
      421 ccgcgcgcgg tgagtccctt tcgggagcct cgcgcctggc aacgcaggcg agctcgcccc
      481 tgacggagcg cggcacggtg ctcgggacgt tccagtacat ggcgcctgag cagctcgaag
      541 gcggagaggc cgacgcccgc tcggacattt tcgcgttcgg cgccgtgctg tacgagatgg
      601 cgacgggaaa gaaggccttc acgggcaaga gccaggcgag cctcgccggc tcgatcctcc
      661 gcgacgaccc gccctcggtc aacgaagtcg cgcccatgac gccgccggcg ttgaaccgcg
      721 tcatcaagac gtgcctcgcc aaggacccgg aggatcggtt ccagacggcg cacgacgtca
      781 agctgcagct ccagtggatc gcggagggcg gatcgcaggc gggactgccc gcgccggtcg
      841 ccgcccgacg caagagccgc gagcgcctgg cgtggggcgt cgccgccgcg gcactcgtcg
      901 ctgcggccgc actcggattc ggcttcgtgc gccgggcgcc ggcaccgcgg cgctcggtcc
      961 ggttcgaggt cgccattccg gagggggtcg tcgcgatcga tgcgccacgc atctcacccg
     1021 acggccggta tctcgccttc aacgcgacgg acgccgaggg caagacccgg atctggctcc
     1081 ggcctttgaa cgctctcgcg gcgcagcccc tcccgggcac ggatgggacg acgcggccct
     1141 tctggtcgcc cgacagccgc ttcctgggat tcttcgcaga agggaagctg aagaagatcg
     1201 aggtcaccgg gggcccggcg cagaagatct gcgacgcacc gaccggcgcc gacggaagct
     1261 ggagcccgga cggtgtcatc ctcttcgacg gcaccggaaa cgacccgatc aaccgcgtgt
     1321 cggcggccgg cggcacgccg acggcggccg tgaaagctga caaggacgtc caggtcggct
     1381 ggcccgagtt cctgccggac ggccgtcact tcctctatat gtccatcggc accaagcagg
     1441 aggacagcgc ctaccgagtg ggttccctcg actcgaccga gaccaagcgc ttcgcgccgg
     1501 cgcagaccct cgtgacgtac gcgccccccg gctacctgct cttcgtccgc gacaggactc
     1561 tcgtggcgca gcagttcgat gcgaaggccg tcaagacgaa gggcgagccg atccctctcg
     1621 ccgagcagat cgggaccgac aacgtcggtc tcgcgacctt ctccgtgtcc cgggaaggga
     1681 cgctcgccta ccgaacggga gagacgggca accgcctgct gtggatggat cgctccggaa
     1741 aggagctcga ccccgtcggc gatcatggcc agtactcgaa ccccgtgctt tccccggcag
     1801 gcgaccgcct tgccttcggt ttgaacgatc ctcgcaacgg aaacaacgac atctggatcc
     1861 gggatctcgc gcgcggggtc agttcccgct tcacgttcgg ggtgggagcc aacaatggag
     1921 tcccgatctg gtctcccgac ggcaacacga tcgtgtttcg gtccgaccgc gaagggaccc
     1981 tcaatctcta cgaaaaggcg gcgagcggac agggggagga gaagatcctg ctgaagaccg
     2041 acgagatcaa attcgctacg gactggtctc gagacggccg ctacatcgcg ttctcgagcc
     2101 agaacacgaa gaccggctgg gactcgtggg cgcttccgac gttcggcgac cgcaagccgt
     2161 tcccgatcgt tgcgggccag ttcgcggagt tgaacccgat gttttcgccg gatacgcgat
     2221 tcgtcgcgta tcagtcgaac gagtcgggga acgcggagat ctacattcag accttccccg
     2281 tcgccagcgg caagtggcag gtttcgaccg ggggcggtac ggacccttca tggcgcgccg
     2341 acggcaaaga gctctactac cgctcggcgg accagaagct catggcggtc gagattcagg
     2401 ccggcgaaac cctcaaggcg ggcatcccgc agcccctctt tccggcacgg gtccggacgg
     2461 gcacggcgcg gaacaagtat ctggccacga aggacgggca gagattcctc ctggtcgcac
     2521 cgttgggtcg tgaggccttg atcccgacga ccgtcgtcct gaactggaac gccgacctcg
     2581 ggcgctgacg tttcccgggg gtcgaagcgt gagcgctttg acactcgcag ccgatctcga
     2641 ttagcgtcgc gccatgactc ttccgaccga gcccgtcggc agcattccgc gccctcccga
     2701 gctcatcgaa gggctgaagg ggagccttcc ggaggatcgg atggactccc tcttcgacgc
     2761 cgcggtgcgc gacacgatcc ggcgtttcga ggagaccggt tcgactgtca tcaccgatgg
     2821 cgagcagcgt aagccttcct tcgccaccta ccccatccat ggcgcaaaga acatcggtcc
     2881 cgggggagtg accatcccgt tcaaggacgg ccacacgagg cagcttccgg tcttgaccgc
     2941 gggtcccttt cgctacgcga cctacgcctc cagctatctc gaggcagccg ggaagtacgc
     3001 gcggaccccg ctcaaacagg ccgtcatttc cgcctcggcc ctgagtcttc tctatccgca
     3061 ggccggcata ccgggataca cgcgcgagga gttcctggac gacctcgttc gcgaagcgca
     3121 gacggacatc cgcaggtgtc tcgatgcggg ggcgacggtg cagatcgact tcacggaagg
     3181 gcggctcgcc gtgaagctcg atccttcgaa gaacctgctc aattccttcg tggacctgaa
     3241 caaccgcgtg ctcgcggcgt tttcggccga ggagaggcgg cggatcgggg tccacacgtg
     3301 tcccggcggc gaccaggact cgacgcacag cgccgatgtg gactatgccg agctcttgcc
     3361 ggccctcttc cggttgaacg tcggaaactt ctacgtccag ctttcgagcg aaaaggaccg
     3421 accccgcgtc cttcgcatcc tcggcgagca cgcgcggggt gatcgaagaa tcttcgtcgg
     3481 cgtcaccgac ccgatcgatc cgaggatcga gaccgccgat gaggttcgcg atcgcgtact
     3541 cgaggcggcg aagttcatcg atccgggcca cctgggcacg acggacgact gcggtttctc
     3601 gccgttcggc gacgacacct cgacctcccg ggacaccgcg ttcgccaaga tccgggcgcg
     3661 cgtcgagggc acgcagatgg cctcgcgggc gctcggcgtc tgacgccgtt cagcgggcct
     3721 ttcgacgtcg caatcgagga tcgaaccgga acgccgcctt cttctcgatc cggatgcggc
     3781 gggcatcggg atgccggggg ttcacgagcc agttcttctc ttcggggatc acgcaggagg
     3841 ggacgatcag gaccgcggac tcgagcgagc tcgcccagtt cgttccgatc gcggcgagtc
     3901 tctccggcgc cggatatgtc ctccagctgg ccggcagctc gccgatgccg agctcccgcc
     3961 ggctcacagt cgcgggcacc tcgacggaga tggaaaccag atcggcgggc gcctcttcga
     4021 ggccgacgtt gacgaggtac tcgagcgccg ccagggagag ggttgccgac gtgtacacca
     4081 tcggaattcc cttggagttc cagcgaccgc cggccgcgcg ggcgccttcg ccgtcgagag
     4141 cggagtggcg tcgcgaagcg atccgccaga cgagcaacta gctgtagacg ccgtgggcga
     4201 tccggagcag gaggtcctcg acctcgcggg atccgagatc cgtgtccagc cgatccaggg
     4261 gagcccggtc gccgagcgcc cggttcttct tcttcagcca gctcgccgcc ttttcgcggc
     4321 ttccgagcgt gacctccgcg agcgtggcga ccctcccgat ccgcatgagc cggtccgatt
     4381 cgtccgggcg cagcctgcgc tcgttcttcc ttcgcgcgag agtccgttcg gggaggcgca
     4441 aaatcgcgcg catcgcggcg agtccgatct cgaggttcga gctcagagcg tcgagggatt
     4501 cgtagcgcag cccttcttcg actctccgcc tcagatcgtc gaaggttccg acggaccccc
     4561 gcagggctct cggtcccccc agcgtctccg cgatcgcgcg agcagtcacc gcgctccggc
     4621 gtgccatgtg acaaggataa cacagccaga tggctgtgac tccgcagcag gacggcttgg
     4681 acacctcgag gaatcccggt gagcgacgcg accccgggag acaccctttc ccgacgaccg
     4741 ctcggattct ggtcggcggc ggctctcgtc gtcggtcaca cgatcggcgt cggcgtcttc
     4801 ctgacgcccg tggagttgat cggtgcgctc ggctcgccgg cgtggaccct cggactgtgg
     4861 ctcacctgcg gtgccctcgt cgtctgcggc gcgttcacct tcggcgagct cgtgtcgcgc
     4921 tttccgcaga cgggcggtct ttacgtctac ctgcgcgagg cgttcggcgg ccgcgccgct
     4981 ttcctgtacg gctggctgtg cctgctcgtc gtggaccctg gcgtgaccgc agcgctcgcg
     5041 gccggcgtgt cgccgtacgc cgccgttctc tggcccgcga tggaaggtcg ggaaaaggtg
     5101 ttcgccgtcg gtctcgtctg ggccctcgcc ctcgtcaaca tggcggggtt gaggccgagc
     5161 gcacggttct tcaccggcct caccctcgtg aagatcctcg cgctcgcggg ggtcgtcgtg
     5221 ctggcgttcg cggccgggtc cggcagctgg tcgcacttcg tgccgttcgc cccccggcac
     5281 ccgggagctc ccccgatcgg ggaagcgttg gggctcgccc tcgtcggcgc cttcttctcg
     5341 ttcgggggct tctgggaggc gagccgcgtc gccggcgaga cgcgcgagcc cggtcggacc
     5401 ctcccacgcg cgctggctct cggcgtcgct acggtgacgc tcgcgtacgt cgcaacgacc
     5461 ctggcgttcg tgtacctcgt cccggtcgag gggatgacga gccccgtcga gttcgctcgc
     5521 cgtgcaggcg aagcgatgct cggccacgcg ggcccttcgg ccttcgcggc agtcgtcgtc
     5581 ctttcggt
//
LOCUS       DASWAV010000326         3396 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U2665
            k141_537823, whole genome shotgun sequence.
ACCESSION   DASWAV010000326 DASWAV010000000
VERSION     DASWAV010000326.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960316
            Sequence Read Archive: SRR10260007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 3396)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3396)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:47:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,929
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3396
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_537823"
                     /isolate="SMAG_U2665"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.36444 N 132.31889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1086
                     /locus_tag="VF958_06690"
     CDS             <1..1086
                     /locus_tag="VF958_06690"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: protein motif:HMM:NF003766.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008973 - phosphopentomutase activity
                     [Evidence IEA];
                     GO_process: GO:0009117 - nucleotide metabolic process
                     [Evidence IEA];
                     GO_process: GO:0043094 - cellular metabolic compound
                     salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HEX9148834.1"
                     /translation="GDAGADTLGHVLDRIPTALPNLESLGLLALLDPSRGGRAAGARG
                     KAYELSAGKDTTTGHWEMMGLVVERPFPLYPDGFPPEIVEPFEKYLGKKVLGNKAASG
                     TEIIQELGAEHQATGRPILYTSGDSVFQIAAHEEVWPPERLWEVCRFARSILVGDHAV
                     GRVIARPFVGKPGAFVRTANRRDYSVEPIGDTWLDRLVAAGRHVFGVGKIADIFSGRG
                     ITSSVHTGSDAEGVRVSVEALTSGDADVVFTNLVDFDSKYGHRNDPAGFAKNLAGLDA
                     LIPRVLSALAPADLFFLTADHGCETTDVSTDHTREHVPFLSAGPAVRGDVDLGIRAGF
                     ADLAATAGEWLGVPAPRGKSALSEILA"
     gene            1101..2240
                     /locus_tag="VF958_06695"
     CDS             1101..2240
                     /locus_tag="VF958_06695"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX9148835.1"
                     /translation="MAGVAVTVLGAVVLPLNLAAFPRLSVAAGVASPHRCPRVSIVIP
                     ARNEELDIERAVRSHLSQDYPELEIVVVDDRSTDRTAQILDRLVREDPRLVVLTGTDP
                     PEGWLGKPHALWQGAAVADGELLLLADADVRYDPRALRDAVTVLEDRRLDLLAFFPRF
                     ENRGFWENVLLPFLAVAVFLGFGFLALSRRVPLAMGAGAGNLVRRRAYDAVGGHAAIR
                     DAVVDDVRLAVVVKRAGFRVAAFRAEDRVAVRMYRGFFEVWNGFTKNVAWAYSGPGGA
                     LLFGLTVLLLAVGIAPVATLVAAAAGVPILRADVVLAAAVFAGSVLLRASLAATLRDP
                     LWPAATHPIMAAVWAGLLGRSLFDRFVRKRLTWRGREYDARRARS"
     gene            2267..>3396
                     /locus_tag="VF958_06700"
     CDS             2267..>3396
                     /locus_tag="VF958_06700"
                     /inference="COORDINATES: protein motif:HMM:NF019285.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside transporter C-terminal
                     domain-containing protein"
                     /protein_id="HEX9148836.1"
                     /translation="MRWIGLAGVVVLFGIAYLFSANRRAIRWYTVLWGFALQVLFAFL
                     VLYWEAGKNALERFSLAITGAISYADQGGAFVFGWLTGRMEKVGQATGLPIGGYIFAF
                     KVLPIIIFIASFFTIMYYLGVMQKIVQGMAWVMQRLMRVSGAESLAVAANVFIGQTEA
                     PILIAPYIPTMTRSELLTMMTGGMAHVSGAVLLAYVAMGIPAKYLITASVMAAPGAIM
                     MAKILMPETEVPVTMGEVKLKVEKTHTNLIDAAASGASQGMTLVINIVAMLIAFIALI
                     ALLNGFLGWVGGLFGHPEFQLQQVFAWVLWPLAVVMGVPPRDAGAVADLIGNKTVINE
                     FFAYAKMSTVQGGMSEKARLIASFALCGFANFSSIGIQIGGIG"
BASE COUNT          482 a         1128 c         1146 g          640 t
ORIGIN      
        1 ggcgacgccg gcgccgacac gctcggtcat gttctcgatc gcataccgac cgcgctgccg
       61 aatctcgagt cgctcggcct gctcgctctc ctcgatcctt cccgcggagg ccgggcagcg
      121 ggcgcccgcg gaaaggccta cgagctctcg gcgggaaagg acacgacgac gggtcactgg
      181 gaaatgatgg ggctcgtcgt cgagcgtccg ttcccgctct atccggacgg gtttccaccg
      241 gagatcgtcg agcccttcga gaagtatctc ggaaagaagg tcctcggcaa caaagcggca
      301 tcgggaaccg agatcattca ggagctgggc gcggagcatc aggcgaccgg gcgcccgatt
      361 ctctatacgt cgggcgattc cgtcttccag atcgccgcgc acgaggaggt ctggccgccg
      421 gaacgtctct gggaggtctg ccgattcgcc cggtcaatcc tcgtcggtga ccacgccgtc
      481 ggtcgcgtga ttgcgcgccc ctttgtcggg aagcccggcg ccttcgttcg cacggccaac
      541 cggcgggact actccgtcga acccatcgga gacacgtggc tcgatcgact cgtcgccgcc
      601 ggcaggcacg tcttcggggt cggcaagatc gccgatatct tttcggggcg cggcatcacg
      661 tcgtctgtgc acacaggctc cgacgcggag ggcgtccgcg tttcggtcga agcgctcaca
      721 tccggcgacg ccgacgtggt cttcacgaat ctcgtcgact tcgacagcaa gtacggccac
      781 cgcaacgacc ccgccggatt cgcgaagaac ctcgccggcc tcgatgctct catcccgaga
      841 gtcttgtccg cactcgctcc cgccgacctc ttcttcctga ctgccgacca cggctgcgag
      901 acgaccgacg tctcgacgga ccacacccgc gagcacgttc cgtttctctc ggccggaccc
      961 gcggtgcggg gcgacgtgga cctcgggatc cgagcggggt tcgccgacct cgcggcaacg
     1021 gccggagagt ggctcggggt ccccgctccg cgcggcaaga gcgcactttc cgagatcctc
     1081 gcctgatcca cgccctgctc gtggcgggcg tcgcggtgac cgttctcggc gcggtcgttc
     1141 tgcccctgaa cctggcggcg tttccgcggc tttcggtcgc cgcgggtgtg gccagtccgc
     1201 accgctgtcc gcgtgtctcc atcgtcatcc cggcgcgcaa tgaagaactg gatatcgagc
     1261 gggcggtccg ctcgcatctg tcgcaggact acccggagtt ggagatcgtg gtggtggacg
     1321 accgctcgac cgaccgcacc gcgcagattc tggaccgtct cgtgcgggag gacccacgcc
     1381 tcgtcgttct caccgggacg gatccacccg agggatggct cggcaaaccg cacgccctct
     1441 ggcagggagc cgcggtcgcg gacggcgagc ttctgctcct cgcagatgcc gacgtgcggt
     1501 acgaccctcg agcgcttcgt gacgcggtca ccgtcctgga ggaccggcgg ctggatctcc
     1561 tggccttctt tccgcgattc gagaaccgcg gcttctggga gaacgtgctc ctgccgttcc
     1621 tcgccgtcgc ggtgtttctc gggttcggat tcctcgcgct ctcgcggcgg gtccctctcg
     1681 cgatgggagc cggcgccggg aacctcgtgc ggcgccgcgc ctacgacgcg gtcggaggac
     1741 acgccgcaat ccgtgatgcg gtcgtggacg acgtgcgtct cgccgtcgtg gtgaagcggg
     1801 ccggctttcg ggtcgcggcc ttccgcgcgg aggaccgcgt cgccgtccgg atgtatcggg
     1861 ggtttttcga ggtgtggaac gggttcacga agaacgtcgc gtgggcctac agcgggcccg
     1921 gcggagcttt gctcttcggt ctcacggtgt tgcttctggc cgtcgggatc gcgccagtcg
     1981 cgacgctcgt cgccgcggcc gccggagttc cgattctccg cgccgatgtc gttctcgccg
     2041 ccgctgtctt cgcgggcagc gttcttcttc gagcttcgct cgccgcgacg ctccgcgatc
     2101 ctctctggcc ggcggcgacg caccctataa tggccgccgt ctgggccggc ctgctgggcc
     2161 ggtctttgtt cgacaggttc gttcgaaagc gcctcacctg gcggggccgg gagtacgacg
     2221 cgcgccgcgc gcgcagctag cgagagctgg cgagaggagg tcggggatgc ggtggatcgg
     2281 cctcgcgggg gtcgttgtgc tcttcgggat cgcctacctc ttctcggcaa accgccgtgc
     2341 gattcgctgg tacaccgtac tgtggggatt cgcgctccag gtccttttcg cctttctcgt
     2401 cctttactgg gaggcgggca agaacgcgct cgagaggttc tctctcgcga tcacgggcgc
     2461 gatttcctac gccgaccagg gaggcgcgtt cgtcttcggc tggctgaccg gccggatgga
     2521 gaaggtcggg caggcgaccg ggcttccgat cggcggatac atcttcgcgt tcaaggtgct
     2581 gccgatcatc atcttcatcg catcgttctt cacgatcatg tactacctcg gcgtgatgca
     2641 gaagatcgtg cagggcatgg cgtgggtgat gcagaggctc atgcgcgtgt ccggcgccga
     2701 gtcgctcgcc gtcgcggcga acgtgttcat cggccagacg gaggcgccca tcctgatcgc
     2761 accgtacatc ccgacgatga cgcgatccga gctcctcacc atgatgacgg gcggaatggc
     2821 gcacgtttcg ggggcggtcc ttctcgcgta cgtcgcgatg ggcatcccgg cgaagtacct
     2881 gatcacggcg tcggtcatgg ccgcgcccgg cgcgatcatg atggccaaga tcctgatgcc
     2941 ggagacggaa gtcccggtga cgatgggaga ggtgaagctc aaggtcgaga agacgcacac
     3001 gaacctgatc gacgccgccg cgtccggcgc gtcgcaaggg atgacgctcg tcatcaacat
     3061 cgtcgcgatg ctcatcgcct tcatcgcgct gatcgcgctc ctgaacggct ttctcggctg
     3121 ggtcgggggt ctcttcggac acccggagtt ccagcttcag caggtcttcg cgtgggtcct
     3181 ctggcctctg gcggtcgtca tgggggtccc ccccagggac gcgggagccg tcgccgacct
     3241 gatcgggaac aagacggtga tcaacgagtt cttcgcctac gcgaagatga gcaccgtcca
     3301 ggggggaatg tcggagaagg cgcgcctcat cgcgtcgttc gcgttgtgcg ggttcgcgaa
     3361 cttctcctcg atcggcatcc agatcggagg gatcgg
//
LOCUS       DASWAV010000327         1856 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U2665
            k141_97524, whole genome shotgun sequence.
ACCESSION   DASWAV010000327 DASWAV010000000
VERSION     DASWAV010000327.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960316
            Sequence Read Archive: SRR10260007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 1856)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1856)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:47:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,929
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1856
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_97524"
                     /isolate="SMAG_U2665"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.36444 N 132.31889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..197)
                     /locus_tag="VF958_06705"
     CDS             complement(<1..197)
                     /locus_tag="VF958_06705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9148837.1"
                     /translation="MKKIAIALTFVVCVAVPAAAQQFGKNKIAYDRFDWKIYRSTHFA
                     IYFYGSEGVSLPKVTSYAESA"
     gene            complement(250..954)
                     /locus_tag="VF958_06710"
     CDS             complement(250..954)
                     /locus_tag="VF958_06710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9148838.1"
                     /translation="MDRRTRIGALALAVLLSALPALAGVAQVKLKLPLKPRLPLRGNE
                     KIALAPFVLATEAEKARDKRLQNVDLQAEFQRFLKKQIEKKTKLEVVELPTDLKLPTQ
                     NLSELGRSTEYWRALGAQTGAEILVSGSLEFRVEDRSGYKTEEYVSPIDGRTYYRQVY
                     VEQTGFLFDIVFLVFDGHSGEKILQDEFKDFRQTGGRRADELQGLFENLFSLENQILN
                     LFVARDREAERYIFTD"
     gene            complement(944..1309)
                     /locus_tag="VF958_06715"
     CDS             complement(944..1309)
                     /locus_tag="VF958_06715"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX9148839.1"
                     /translation="MKKLALVLTAVLITAGCSSYARTDKAATQISFGSAVARKGLWRE
                     AAFRFEKAVEKEPGNARAHNNLAVALEASGEFARALGEYKRALELDPNDSYIRRNYAR
                     FAEFYTSYTRTTGKATGGS"
     gene            1474..>1856
                     /locus_tag="VF958_06720"
     CDS             1474..>1856
                     /locus_tag="VF958_06720"
                     /inference="COORDINATES: protein motif:HMM:NF014533.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HEX9148840.1"
                     /translation="MAIDALARRAAVSWARSRDLTPQRLWPVLRDRGGLDALLATPEA
                     DLADRLASRERARAVLAGPSDSAAERWAASVETSGIRIVTAFDAEYPRALLEIADPPF
                     LLFSVGRLEKFRLPAVAVVGSRSGTR"
BASE COUNT          302 a          600 c          599 g          355 t
ORIGIN      
        1 taagcgctct cggcgtacga cgtgaccttc ggcagcgaaa ccccctccga cccgtagaag
       61 taaatggcaa agtgcgtcga gcggtagatc ttccagtcga accggtcgta ggcgatcttg
      121 ttcttgccga actgctgcgc cgcggcgggg acggcgacgc aaacgacgaa cgtgagcgcg
      181 atcgcgatct tcttcatgag gcctcgctgc tctatcggaa tcaagaggtg gtggatcggc
      241 aggcgtggat cagtcggtga agatgtatct ctccgcctcg cggtcgcgcg ccacgaagag
      301 gttcaggatc tgattctcga gcgagaagag gttttcgaaa aggccctgca gctcgtcggc
      361 gcggcggccc cccgtctggc gaaagtcctt gaactcgtcc tgaagaatct tctcgccgga
      421 atgcccgtcg aataccagga acacgatgtc gaaaagaaat ccggtctgct cgacgtagac
      481 ctggcggtag tacgtccggc cgtcgatggg ggaaacgtac tcctccgtct tgtagcccga
      541 ccggtcctcg acgcgaaact cgaggctacc ggaaaccagg atttccgctc cggtttgagc
      601 gccgagcgcc cgccagtact ccgtcgaccg gccgagctcc gacaggttct gcgtcgggag
      661 cttgagatcg gtcggcagct cgacgacttc gagcttcgtc ttcttctcga tctgcttctt
      721 cagaaatcgc tggaactccg cctggaggtc cacgttctgg agtcgcttgt cgcgcgcctt
      781 ttcggcctcg gtcgcgagga cgaaaggcgc cagtgcgatc ttttcgttgc cgcgcagcgg
      841 gagccgcggc ttcaggggca gcttcagctt gacctgcgcc actcccgcca gagccgggag
      901 cgcggaaagg agcaccgcca gggcgagcgc tccgatgcgg gtcctacgat ccaccggtcg
      961 cctttccggt cgtccgcgtg tacgacgtgt agaactcggc aaagcgcgcg tagttgcggc
     1021 ggatgtagga gtcgttcggg tcgagctcca gcgcgcgctt gtattctccg agagcgcgcg
     1081 cgaactcgcc gctcgcttcg agggcgacgg cgaggttgtt gtgcgcccgc gcgttgcccg
     1141 gttccttttc gacggctttc tcgaatcgga aggccgcctc ccgccagagg cccttccgcg
     1201 cgacagcgct gccgaaagag atctgggtgg ccgccttgtc cgtgcgcgcg taggagctgc
     1261 acccggcggt gatcaggacg gctgtcagaa cgagcgcgag ctttttcacc caaacctctg
     1321 aacccccaca ggggggcggc tattataggt aaggcgtttc gccatcgcgg aatttcgtcg
     1381 ccgtgatcgt tcccccgggc ccgccttcct caagaacaga tgaaaaacgc tcctgccggt
     1441 ccgatccgcc ttccgagaat atcttttaga ggcatggcga tcgacgctct cgcccgacgg
     1501 gccgcagtct cctgggcccg atcacgggac ctgacaccgc agcggctctg gccggtcctg
     1561 cgggatcggg gtggtctcga tgctctcctg gcgacgccgg aagcggacct cgccgatcgg
     1621 ctggcctccc gggaacgcgc acgcgcggtc ctcgccgggc cgtcggactc cgccgccgag
     1681 cggtgggcgg cgtccgtcga gacctcggga atccggatcg tgacggcatt cgatgccgaa
     1741 tacccccggg cgcttctcga gatcgccgac cctcccttcc tcctcttctc cgttggccgg
     1801 ctggaaaagt ttcggcttcc ggcggtcgcg gtcgtcgggt cacggagcgg aacgcg
//
LOCUS       DASWAV010000328         4028 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U2665
            k141_418302, whole genome shotgun sequence.
ACCESSION   DASWAV010000328 DASWAV010000000
VERSION     DASWAV010000328.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960316
            Sequence Read Archive: SRR10260007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 4028)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4028)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:47:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,929
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4028
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_418302"
                     /isolate="SMAG_U2665"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.36444 N 132.31889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..228
                     /locus_tag="VF958_06725"
     CDS             <1..228
                     /locus_tag="VF958_06725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9148841.1"
                     /translation="VRDIADGFIIPTELTFKKFGPGDLVVFSQEADKFLREVRGSTPA
                     TNDVEETRKRQRRLQRLQQAMSLARGAQSRR"
     gene            360..2588
                     /locus_tag="VF958_06730"
     CDS             360..2588
                     /locus_tag="VF958_06730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9148842.1"
                     /translation="MRSGLGDLWDAKLNAWIFHWDFHQTFRDPLHLFDANIFHPARYA
                     LAFSENLFGAAVFGFPLFASGASTLFVYNFLFLLGMFFSAVGAWALARRVTGDPAASL
                     LAGCVYAFVPWRLAQIPHVQYQWGAFLPLLLLFLLRWLDGGRRRDLVLFGVFFAWNAV
                     TNVHYAIFSGILLAVVLAWDALTSGDARIGWRIRRAVGATAIAAIVVLPFYVPYAKAA
                     KLYGMRRSAGEMRFYSARPSAFLVAGPQNKLWSPLTQRFARPEAEMFPGIVPLALAVY
                     SVVRLRRRRQAGPAARSEIAPWRRRAARVLDGLSAVAFAAGIAGLAIRGLRIGPINLA
                     DPGRALVFLTVFVLLRLLLAFPGRSVHANLRDFLRTRRLDRRAGLFVAIGFAGLLIAA
                     GGYTPYYTFLFQSFGFVFRAIRVPARGMVLFHLALAVLAAWGLSLLVRGVGRRAERAG
                     FLAAALLLTAIEYRAAPIDFPAVEEAPAPVYRWLAHVPVPGAVVEWPIGFDWDAEHVF
                     RSTAHWKKLINGYSGFAPKHYDELKTLLEESPIPERVWARIREFGGAVLVLHPHEVEG
                     PARLNYARGARKALVEGKLEMLGSFPHDAATDFVFRIAPAPAFSTGIAPADRDRSAAE
                     FERLTSVAESELAPPFGVIDAPVEGAEIAGGTWGYGWALDDSGIARVEVATELGPAGL
                     GVVGQPHPGIAEIFPNYSERDRAGFGFIVPQVARGPHTLLLTLVARDGGVTVLKRQIR
                     VR"
     gene            2680..3417
                     /locus_tag="VF958_06735"
     CDS             2680..3417
                     /locus_tag="VF958_06735"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX9148843.1"
                     /translation="MPCRIRDALPDLLETLHLTSSASLPPPRLRKRVGRTSSRREFLS
                     VGRAAARDVLAAFEDVRNPASDWGRWLDFGCGAGRVARFIGVFPIVRTMRGIDVDAEA
                     IRWNRRHMTGDYLLLARDASLPFGDKSFDVVFSVSVFSHLDESPQFAWLREIHRVLRP
                     GGLFLASTHSDKLRYSRPDLTFEQHLALNSRGFLFARGRGEFKTDSAFHSRLYLEQQW
                     GRWFSLRLHREFGLAGFQDLTVWERPV"
     gene            3430..>4028
                     /locus_tag="VF958_06740"
     CDS             3430..>4028
                     /locus_tag="VF958_06740"
                     /inference="COORDINATES: protein motif:HMM:NF013499.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OB-fold nucleic acid binding domain-containing
                     protein"
                     /protein_id="HEX9148844.1"
                     /translation="MSDPETTAISHIGKRTGSEATVRGWLYNRRSSGKIQFLIVRDGT
                     GYLQTVVAKADVSPEIWAEAERAAQECSLEVRGRVRADPRAPGGFEMTASDVRILGPS
                     ENYPISPKEHGVDFLMGLRHLWMRSAQQHAVLRVRSEVAQAIRDFFYERQYTLIDSPI
                     LTGSSVEGTSTLFETDYFGDKAYLSPSGQLYLEPAAAAFG"
BASE COUNT          543 a         1374 c         1344 g          767 t
ORIGIN      
        1 gtgcgggaca tcgcggacgg tttcatcatt ccgaccgagc tcactttcaa gaagttcgga
       61 ccgggcgacc tcgtcgtctt ctcgcaggag gcggacaagt tcctgcggga ggtgcgcgga
      121 agcacgcccg ccacgaacga cgtcgaggag acgcggaagc gacagcggcg attgcagcga
      181 cttcagcagg cgatgtcgct cgcacggggc gctcagagcc ggcgctgatc gagcgcgcga
      241 tcgccggagg ccggagcccg gaccaggggg ctcacccccc tcggtggagc gagaccgctc
      301 tcgcggcgct cttctttttc gttgcgaccg ttctcgccac ctggccccag gcctcccgga
      361 tgcgcagcgg cctcggcgac ctgtgggacg cgaagctgaa cgcatggatc ttccactggg
      421 atttccacca gacgttccgc gacccgctcc acctcttcga cgccaacatc ttccatccgg
      481 cgcgttacgc gctcgcgttc tccgagaacc tcttcggagc tgcggtgttc ggcttcccgc
      541 tcttcgcgtc gggcgcgtcg accctcttcg tgtacaactt tctctttctc ctcggcatgt
      601 tcttttcagc cgtgggggcg tgggcgctcg cccgccgtgt caccggggat ccggcggcct
      661 cgctgctcgc cggctgcgtc tatgccttcg ttccgtggag gctggcgcag atccctcacg
      721 tccagtatca gtggggcgcg ttcctcccgc tcctgctcct cttcctgctg cggtggctcg
      781 acggcggacg gcgccgcgac ctcgttctct tcggggtttt cttcgcgtgg aatgccgtga
      841 cgaacgtcca ctacgccatc ttctccggca tcctgctcgc cgtcgtcctc gcgtgggacg
      901 ccttgacgag tggcgacgcc cgcatcgggt ggcgcatccg tcgggcggtc ggggccacgg
      961 caatcgcggc gatcgtcgtt ctcccgttct acgtccccta cgcgaaggcg gcgaagctgt
     1021 acgggatgcg gcggtcggcg ggggagatgc ggttctactc ggcccggccg tcggcgttcc
     1081 tcgtcgcggg tccccagaac aagctctggt ctccgctcac gcagcgcttc gcccgcccgg
     1141 aggcggagat gtttcccggg atcgtcccct tggcgctcgc cgtttactcg gtcgtgcgac
     1201 tgcgccggcg aaggcaggcc ggcccggcag cgagatccga aatcgcgccg tggcgtcgcc
     1261 gggcggcgag agtcctcgac gggctctctg ccgtcgcttt cgcggcgggg atcgccggac
     1321 tggccatacg cggcctgcgc atcggtccca tcaacctcgc tgatcccgga agggctctcg
     1381 tcttcctgac cgtgttcgtc ctcctgcgtc tcctgctcgc gtttcccggc cggtccgtcc
     1441 acgcgaatct ccgcgacttc ctgcgcaccc gccggctcga ccggcgcgcc ggcctcttcg
     1501 tcgcgatcgg tttcgcgggg ctcctgatcg ccgccggcgg gtacacgccg tactacacct
     1561 tcctcttcca gtcgttcggt ttcgtgtttc gcgcgatccg ggtcccggcg aggggaatgg
     1621 tcctgttcca cctcgcgctc gccgtgctcg ccgcgtgggg actttcgctt ctcgtgcggg
     1681 gcgttggccg ccgggccgag cgggccggat tcctcgcggc ggcgctcctt ctgaccgcga
     1741 tcgagtaccg ggcggcgccg atcgacttcc cggcggtgga ggaggcgccg gcgccggttt
     1801 accggtggct ggcccacgtc cctgttcccg gagcggtcgt tgaatggccg atcggcttcg
     1861 actgggacgc cgagcacgtc tttcgctcca cggcgcactg gaagaaattg atcaacggct
     1921 actccggttt cgcgccgaag cactacgacg agctgaagac tctgctggag gaaagtccga
     1981 tccccgaacg ggtgtgggcg agaatccggg agttcggcgg cgccgtcctg gtgctccacc
     2041 cgcacgaggt tgaggggccc gcgcggctca actacgcccg cggagcgcga aaggccctgg
     2101 tggaggggaa gctggagatg ctcggatcgt tcccgcacga cgcggccacc gacttcgtgt
     2161 tccgcatcgc accggcgccg gctttctcca ccgggatcgc tccggcggat cgggatcgct
     2221 ccgccgcgga gttcgagcgg ctgacgtcgg tcgcggagtc ggagctcgcg cctccgttcg
     2281 gcgtcatcga cgctccggtc gagggcgcgg agatcgcggg cggaacgtgg ggctacggct
     2341 gggccctcga cgactccggg attgcgcggg tcgaggtcgc gaccgagctc gggccggccg
     2401 gactcggcgt cgtgggccag ccgcatccgg gaatcgcgga gatcttcccg aattactccg
     2461 aaagggatcg ggccggattc ggattcatcg tcccgcaggt cgcgaggggg cctcacacgc
     2521 tcctcttgac gctcgtcgca cgcgacggtg gcgtcaccgt gctcaagcga cagatccgtg
     2581 tccgctgaac cccccggctg gaagaagctc ctgcggggcg cggcgagagc cacggttccg
     2641 cagtcggcga gggatctcct tcgttcgctt ctccgcgagg tgccctgccg gattcgggac
     2701 gcgctgccgg accttctcga aacgcttcac ctcacgtcgt ccgcttcgtt gcctcctccg
     2761 cgcctgcgaa agagggtcgg acgaaccagc tcccgaaggg agttcctctc cgtcgggcgt
     2821 gcagccgcgc gagatgttct ggctgcgttt gaggacgtac ggaatccggc atccgactgg
     2881 gggcgatggc tcgattttgg ctgcggcgcc ggtcgagtcg cccgtttcat cggcgtgttc
     2941 ccgatcgttc ggacgatgcg aggcatcgat gtcgatgccg aggcgatccg gtggaatcgg
     3001 aggcacatga ccggcgacta tctcctcctg gcaagggacg cctcgctgcc ctttggggat
     3061 aagtcgttcg acgtggtgtt ctcggtctcc gtcttctccc accttgacga gagtccacag
     3121 ttcgcatggc tccgggaaat ccatcgtgtt ctgcggccgg gcggattgtt cctggcgtcc
     3181 acgcactccg acaagcttcg gtattcgcga ccggacctga ccttcgaaca gcacctcgcg
     3241 ttgaactcga gaggtttcct gttcgcgcgg ggccgcggag aattcaagac cgacagcgcc
     3301 ttccattcga ggctctatct cgagcagcag tgggggcgtt ggttttccct gcggcttcac
     3361 cgtgagttcg gcctggcggg attccaggac ctgaccgtct gggagaggcc cgtataatcc
     3421 gacgctttta tgagcgatcc ggaaacgacg gcgatctccc acatcggcaa aaggaccggc
     3481 tccgaggcga ctgtccgcgg ctggctctac aaccgacgct cgtccggcaa gatccagttc
     3541 ctgatcgtcc gcgacggcac cggctatctg cagacggtcg tcgcgaaggc ggacgtgtcg
     3601 ccggaaatct gggcggaggc ggagcgagcg gcgcaggagt gctccctcga ggtgcgcgga
     3661 agggtccggg ccgacccgcg cgcgccgggc ggattcgaga tgaccgcgtc ggacgttcgg
     3721 atcctggggc cctccgagaa ctacccgatc tcgcccaagg agcacggcgt cgacttcctc
     3781 atgggattgc ggcacctgtg gatgcggtcg gcccagcagc acgccgttct ccgcgtccgc
     3841 tccgaggtcg cgcaggcgat ccgtgacttc ttctacgagc gtcagtacac gctcatcgat
     3901 tcgccgatcc tgacgggatc atcggtcgag gggacctcca cgctcttcga gacggactac
     3961 ttcggggaca aagcctacct ctccccgtcc ggacagctct acctcgagcc ggccgcggcc
     4021 gcattcgg
//
LOCUS       DASWAV010000329         4395 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U2665
            k141_56613, whole genome shotgun sequence.
ACCESSION   DASWAV010000329 DASWAV010000000
VERSION     DASWAV010000329.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960316
            Sequence Read Archive: SRR10260007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 4395)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4395)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:47:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,929
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4395
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_56613"
                     /isolate="SMAG_U2665"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.36444 N 132.31889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..458
                     /locus_tag="VF958_06745"
     CDS             <1..458
                     /locus_tag="VF958_06745"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEX9148845.1"
                     /translation="GEADADDLTQDVFLRAYRGVPRFRDQAPPRAWLAAIANNAIKNR
                     YRSRSRFRRIFSPHPEPDSLADRELDATSPEDNARAGESRHSVLGALSKLPIEFRLPV
                     VLRDLEEWSYEEIATSLALPIGTVKSRIARGRGQLRAILAPLLAPEKNP"
     gene            455..1393
                     /locus_tag="VF958_06750"
     CDS             455..1393
                     /locus_tag="VF958_06750"
                     /inference="COORDINATES: protein motif:HMM:NF024881.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-HC2 domain-containing protein"
                     /protein_id="HEX9148846.1"
                     /translation="MKVNGQTHPARSVDFLSRLHDGELDAGERARFESHRAHCAECRR
                     SVVEFEDAISLFRTARSSPPRSDLAGRILRKVQATNRPRTPFPRRFQIDLGWAALMLT
                     ALFAVLIMTPILVRKNPAATSPAPLTPGPRPPIIVQLPRSDSTAPRHAPETRSRAEDR
                     VADARPAAPQRSREDVPASAAVVGPPKAEAGAGQPLTQSEHAEARKSAAEIASAAPEP
                     SGGGEADASARLESATAPLRLSVREADGFGAPPAVRTVPRMALPPSVRGREYFVLVDP
                     QGIVQGVTADREVSGALSRIRFEAGPRARRLLVRVE"
     gene            complement(1403..2398)
                     /locus_tag="VF958_06755"
     CDS             complement(1403..2398)
                     /locus_tag="VF958_06755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015739180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent alcohol dehydrogenase family
                     protein"
                     /protein_id="HEX9148847.1"
                     /translation="MRAMLLARTARAEERPLALVEMPESEPASGEVSIGVEACGVCRT
                     DLHIVEGEVEARLPIVPGHQAAGTVLTCGSGVEGIRPGDRVGVGWLSSTCGECAFCRS
                     GRENLCRGARFTGRDRDGGYAERMTAPALAAYRLPEGFTARQAAPLLCAGVIGYRTLR
                     TSGAQSGSRLGLFGFGASAHLVIQIARHEGCEVYVFTREALHRELAREMGAAWVGGTE
                     DDPGVLLDAAAIFAPAGEIVPIALARLERGATLAVNAIHMSPIPSLDYEKLYGERVVR
                     SVMNFTRRDAQEFLELAARIPVRAETEVFSLDQANEALVRVARGEVRGAAVLAIR"
     gene            2431..2871
                     /locus_tag="VF958_06760"
     CDS             2431..2871
                     /locus_tag="VF958_06760"
                     /inference="COORDINATES: protein motif:HMM:NF017897.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mpo1-like protein"
                     /protein_id="HEX9148848.1"
                     /translation="MERVDALLADYGSHHRTRGNLACHVFGVTLIVFGITSMLGRFRI
                     GAFNAAEILMAAVVLYYATLDLRLALGMLVAFGAIDLAARATGDWRVGFAAFVAGWIF
                     QAVGHAVYEKNRPAFFKNLLHLLVGPLFLVNELLKVRRAAHPNR"
     gene            2942..3661
                     /locus_tag="VF958_06765"
     CDS             2942..3661
                     /locus_tag="VF958_06765"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013433693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HEX9148849.1"
                     /translation="MFPIRDTIPSRHVPVATWLIIGVNVVVFLLELANPAPVVEQMTY
                     LFGIVPARYTHPEWARSVGFPSSYWPFVTSMFLHGGWLHVIGNMWALWIFGDNVEDRM
                     GPLRFLLFYGICGVAAGLVHLLTNTGSTVPAIGASGAIAGVMAAYFILFPRARIVAMF
                     PIVFWPVFFEVPAFLYLGFWFFVQFFSGATALVSPERASGIAWWAHVGGFATGALTFW
                     IFIRPRARRVPHDTFFTGGIA"
     gene            3658..>4395
                     /locus_tag="VF958_06770"
     CDS             3658..>4395
                     /locus_tag="VF958_06770"
                     /inference="COORDINATES: protein motif:HMM:TIGR00730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HEX9148850.1"
                     /translation="MTDRAISEFPRVLRDLGWSDLPGGEAERVAEITREFVEATLAFR
                     DVEPAVTIFGSSRLGPDDAPYQLARETARLLALEGFNVVTGGGPGVMEAANRGCQEGG
                     GVSIGLNIRLPREQKANPYIDREFDFTYFFIRKYMFMKLSCAFFAFLGGYGTLDELFE
                     MLTLMQTRKIQSYPIILGGHDVEDAIEPLLGKLLQHGTINAADRDLLHRSADPEHAVR
                     IVVESYRRLSQVTNKPEKKPARGAASGP"
BASE COUNT          659 a         1561 c         1436 g          739 t
ORIGIN      
        1 tcggagaagc cgacgcggac gatctgacgc aggacgtgtt cctgcgcgcc taccgcggcg
       61 tgccccggtt tcgcgaccag gcgccgccgc gcgcgtggct ggcggcgatc gctaacaacg
      121 ccatcaagaa tcgttaccgc tcgcggagcc ggttccgcag gatcttttcg ccgcatccgg
      181 agccagactc gctcgcggac cgggaattgg acgccacgag cccggaagat aacgcgagag
      241 cgggagagtc gcggcactcg gtgctcggag cgctgtcgaa gctgccgatc gagttccggc
      301 taccggtcgt cctgcgtgac ctcgaggagt ggagttacga ggagatcgcg acatcgctcg
      361 cgctgcccat tggaaccgtc aagtcgcgca tcgcgcgggg gcgaggacag ctccgcgcga
      421 tcctggctcc gctcctcgca ccggagaaga acccatgaag gtcaacggac agactcatcc
      481 cgcccggtcg gtcgacttcc tctcgcgcct gcatgacggg gaactcgacg ccggcgagcg
      541 cgcccggttc gaatcgcacc gcgcccactg cgccgaatgt cgtcggtcgg tggtcgagtt
      601 cgaggatgcg atctcgctct tccgcaccgc gcgctcgagt cccccgcggt cggacctcgc
      661 gggccggatc ctgcggaagg tccaagcgac gaatcgcccg cgaacgcctt ttccgaggcg
      721 cttccagatc gacctcgggt gggccgcgct gatgttgacc gcgctcttcg cggtgctgat
      781 catgacgccg attctcgtga ggaagaatcc cgccgcgacc tcgccggctc ccctgacgcc
      841 cggtccgcgg ccgccgatca tcgtccagct gccgcgctcg gactcgacag cgccgcgaca
      901 cgcgccggaa acgaggagcc gcgcggagga tcgcgtcgcc gacgctcgac ccgcggctcc
      961 ccagcggtcg cgcgaagacg tccccgcgtc ggcagccgtc gtcggtccgc cgaaagccga
     1021 ggcgggagcc ggacagccgc tcacgcagtc ggagcacgcc gaggcacgaa agtcggcggc
     1081 cgagatcgca tcggcggccc ccgagccgtc cggcggcgga gaagccgacg cgtcggcccg
     1141 gctcgagtcg gcgaccgctc ccctgcgcct ctcggttcgc gaggccgacg gtttcggcgc
     1201 acccccggcg gtccgcacgg tcccgcgcat ggcgctccct ccgagcgttc ggggacgcga
     1261 gtatttcgtc ctcgtcgacc cgcaggggat cgtccaggga gtgacagccg atcgtgaggt
     1321 atcgggcgcg ctctcgagga ttcgcttcga ggcgggaccg cgcgcgcggc ggctcctcgt
     1381 ccgggtcgag taagcgccgc cctcagcgga tcgccagcac ggcggcgccc cggacttcgc
     1441 cgcgcgcgac ccggacgagc gcttcgttgg cttgatcgag cgagaagact tcggtctccg
     1501 cacgtacggg gatccgggcg gcgagctcga ggaactcctg cgcgtcgcgg cgcgtgaaat
     1561 tcatcacgct tcgcacgacc cgctcgccgt agagcttctc gtagtccagg gatgggatcg
     1621 gactcatgtg gatcgcgttg acggcgagcg tcgccccgcg ctcgagacgc gcgagcgcga
     1681 tcggcacgat ctctcccgcg ggagcgaaga tcgccgcggc gtcgaggaga acgccggggt
     1741 cgtcctcagt tccgcccacc caggccgctc ccatctctcg tgccagctcg cggtggagcg
     1801 cctcgcgcgt gaagacgtag acctcgcacc cctcgtgccg tgcgatctga atcacgaggt
     1861 gcgcggacgc gccgaacccg aagagaccca gccggctgcc ggactgcgct ccggaggtcc
     1921 gcagcgtccg gtacccgatt acgcccgcgc acaggagagg cgccgcctga cgcgcggtga
     1981 aaccctccgg cagccggtac gccgcgagcg cgggcgccgt catccgttcc gcgtaccccc
     2041 cgtcgcggtc gcggccggtg aagcgggcgc cgcggcagag gttctcccgg ccggaccggc
     2101 agaaggcgca ctcgccgcac gtcgaggaca gccagcccac cccgacccga tcgccgggcc
     2161 ggatcccttc gacgccgctt ccgcaagtga gcacggttcc ggcagcctgg tggcccggca
     2221 cgatcggaag gcgcgcttcg acctctccct cgacgatatg cagatccgtg cggcagacgc
     2281 cgcacgcttc cactccgatc gacacctccc ccgacgccgg ctcgctttcc ggcatctcga
     2341 cgagcgcgag cggcctctct tcagcacgcg ccgtccgcgc gaggagcatg gctctcatcg
     2401 tgcgctattc tccgcgaggc ggaggaccgg atggaacggg tcgatgccct cctcgcagac
     2461 tacgggtccc accaccggac ccgcggcaat ctcgcctgcc acgtcttcgg tgtcacgctg
     2521 atcgttttcg gaatcacgtc catgctcggg agattccgga tcggggcgtt caacgcggcg
     2581 gagatcctca tggccgcggt cgtcctctac tacgcgacgc tcgacctccg gctcgcgctc
     2641 ggcatgctcg tcgccttcgg ggcgatcgat ctggccgcgc gtgcgacggg tgactggcgc
     2701 gtcggattcg ccgcgttcgt cgccggctgg atcttccagg ccgtcggcca tgccgtctac
     2761 gaaaagaacc gacccgcgtt cttcaagaac ctgctgcacc tgctcgtcgg ccccctcttc
     2821 ctcgtcaacg agctattgaa ggttcgccgc gccgcccacc ccaaccgata gatctccctc
     2881 tccctccggg agagggtcgg ggtgaggggt ttgctccacc ctgcgctcct atactcgcgc
     2941 gatgttcccc attcgcgaca cgatccccag ccggcacgtt ccggtcgcga cgtggctcat
     3001 catcggcgtc aatgtcgtgg tcttcctcct cgagctcgcg aacccggccc ccgtcgtcga
     3061 gcaaatgaca tatctcttcg gaatcgtgcc ggctcgctat acgcatccgg agtgggcgcg
     3121 gtcggtgggg tttccctcca gctactggcc gttcgtcacg agcatgttcc tccacggtgg
     3181 ctggctccac gtcatcggta acatgtgggc gctctggatc tttggcgaca acgtcgaaga
     3241 ccggatggga ccgctccgtt tcctcctctt ctacgggatc tgcggcgtcg cggcagggct
     3301 cgtgcacctg ctgaccaaca ccggatctac cgtgcccgcg atcggcgcgt ccggcgcgat
     3361 tgccggggtg atggccgcct acttcatcct ctttccccgg gctcgaatcg tcgccatgtt
     3421 cccgatcgta ttctggcccg tgttcttcga ggtacccgcg ttcctttacc tcgggttctg
     3481 gttcttcgtt cagttcttca gcggtgccac ggccctcgtg tcgcccgaac gggcgagcgg
     3541 aatcgcctgg tgggcccacg tcggaggctt cgcgaccgga gctctcacct tctggatatt
     3601 catccgtccc cgcgcgcgcc gcgtaccgca cgacaccttc ttcacgggag gaatcgcatg
     3661 accgatcgcg cgatctccga gtttcctcgg gtgctccggg atctggggtg gtccgacctg
     3721 ccgggcggcg aggccgagcg cgtcgcggag atcacgcgcg agttcgtgga agcgaccctc
     3781 gcgtttcgcg acgtcgagcc ggcggtcaca atcttcggct cgtcgcggct cggacctgac
     3841 gacgctccgt atcagctcgc gcgcgagacc gcgaggctcc tcgcgctcga aggcttcaac
     3901 gtcgtgacgg gcggcggccc gggagtcatg gaagcggcga accggggatg tcaggagggg
     3961 ggcggggtgt caatcggcct gaacatccgt ctgccgaggg agcagaaggc gaacccctat
     4021 atcgaccgcg agttcgactt cacctacttc ttcatccgca aatacatgtt catgaagctc
     4081 tcgtgcgcat tcttcgcctt tctcggaggg tatgggacgc tcgacgaact cttcgagatg
     4141 ttgaccctga tgcagactcg caagatccag agctatccga tcatcctggg agggcacgac
     4201 gtcgaggacg cgatcgagcc gctgctggga aagctcctcc agcacgggac gatcaacgcc
     4261 gcggatcgcg acctccttca ccgaagcgcc gacccggaac acgcggttcg gatcgtcgtt
     4321 gagagctacc gccggctttc gcaggtgacc aataagccgg agaagaagcc ggcgcgcggg
     4381 gcggcctccg ggccg
//
LOCUS       DASWAV010000330         3774 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U2665
            k141_697532, whole genome shotgun sequence.
ACCESSION   DASWAV010000330 DASWAV010000000
VERSION     DASWAV010000330.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960316
            Sequence Read Archive: SRR10260007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 3774)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3774)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:47:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,929
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3774
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_697532"
                     /isolate="SMAG_U2665"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.36444 N 132.31889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..322
                     /gene="secG"
                     /locus_tag="VF958_06775"
     CDS             <1..322
                     /gene="secG"
                     /locus_tag="VF958_06775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011365892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HEX9148851.1"
                     /translation="AFGGGGSQTAFGARGATTLLHKLTTGAFIGFIVLALLLTVLEAR
                     PRSSVVKTLPQKAATQKAPVPPAQAPAGTGAVPAPAQPATGSPAAPAPAPAPANPPGG
                     PAKP"
     gene            335..419
                     /locus_tag="VF958_06780"
     tRNA            335..419
                     /locus_tag="VF958_06780"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:369..371,aa:Leu,seq:gag)
     gene            511..1344
                     /locus_tag="VF958_06785"
     CDS             511..1344
                     /locus_tag="VF958_06785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9148852.1"
                     /translation="MRRSLSAISLAAALLLVASSARAVYSPATTAGWFITAGRVQGQF
                     GALFRTDLWLFNPDSSASVTLTLTFHPAVGDGAPAAPPINSAPIVLAPRETKFFPDVT
                     FSTVPAGDGVVGALRWQSSAPILGAGRIYTSAPEGTFGFFVPAIPIAESLPRKSATSD
                     AVNVLQIFGVNSGDANFRTNLDVTNTSSVALPIEVRVIDPVTTEIYGGTLNSTVAANS
                     LLRLGAVLSTVGAPQVPGLRITVAVKETAPAFSSGGILAAAYTLDNRTQDAFAFVGQR
                     Q"
     gene            complement(1438..1812)
                     /gene="yidD"
                     /locus_tag="VF958_06790"
     CDS             complement(1438..1812)
                     /gene="yidD"
                     /locus_tag="VF958_06790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005036645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HEX9148853.1"
                     /translation="MPGADSAGTFVAEPAPEAFGRTDRRFRVALAIAALVGFAAGDSL
                     RPADAQSSAKAGVFAIDAYRATLGAVIGKTGIVRCRYEPTCSAYGREAISRYGFPRGF
                     LLTAGRIARCHPFAKGGADPVP"
     gene            complement(1796..2113)
                     /locus_tag="VF958_06795"
     CDS             complement(1796..2113)
                     /locus_tag="VF958_06795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9148854.1"
                     /translation="MSSPRAMLVLCYLAFLGVIPLLFSRDDPEVRWHARNGLLLFGAV
                     VAIALVATLVGTLLPALGCLYATLMFFVLALYAFIAILAIVKALDGQRLLVPGVSKYA
                     GRR"
     gene            complement(2169..2930)
                     /locus_tag="VF958_06800"
     CDS             complement(2169..2930)
                     /locus_tag="VF958_06800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9148855.1"
                     /translation="MRRALLLVGLLLFATVASAEEQVFEKAYSMEGVSRVSVENVNGM
                     IEAYAWDRPYLRVRAVKSARGGRAEETLRLTEIRVRKSGDVIYLETVNPRRRRLFGFL
                     DFGWRNAHVDYEIHLPALAQARLETCNGKVLAIGLGNSIACDAVNGSIELRDVFGPVR
                     ATTVNGSVRIVFRGPMKESRVETVNGSVDVAFDKNSSVRYSLETVNGRIEGDFELAVE
                     GKYGPKEARGTYNGGAETLRCETVNGSIRLKTNTP"
     gene            3017..>3774
                     /locus_tag="VF958_06805"
     CDS             3017..>3774
                     /locus_tag="VF958_06805"
                     /inference="COORDINATES: protein motif:HMM:NF013155.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HEX9148856.1"
                     /translation="MSLFRVADCHLHFEGALPPDTLEALARRAGHRFVDRGVFEAERL
                     AVRSRSEFLLLYREICGLFRSPEDFGDAAIAIARSVAEGGAAYAEIYVSPEIAGRFGL
                     EPTACLVAIDDGFRRAEAEGGTPCRILLDAVRQWGPESADRVLDLHEKTRLPSVVGFG
                     LGGDELALPAAAFAGVYARARALGLRTSVHAGEWGGVESLTEALDHLRPDRVDHGIAA
                     ANDAHLLARFAEESTPLWVSPTSNVVTGAVQDLG"
BASE COUNT          588 a         1319 c         1257 g          610 t
ORIGIN      
        1 cgctttcgga ggtgggggca gccagaccgc gttcggcgct cgcggcgcga cgacgctgct
       61 ccacaagctg acgaccggtg cgttcatcgg gttcatcgtt ctcgcgcttc tgctcaccgt
      121 gctcgaggcg cgcccgcggt catcggtcgt gaagacgctc ccgcagaaag cggcgacgca
      181 gaaagcgccg gtcccgcccg cccaggcgcc ggccggcacg ggcgccgtgc ccgctccggc
      241 gcagccggcg accggttcgc cggcggctcc ggctccggct ccggcgcccg cgaacccgcc
      301 gggaggtccg gcgaaaccgt agtccacgtc cagtgccgtc gtggtggaat tggtagacac
      361 gtacgtttga ggggcgtatg gggcaaccca tgccggttcg agtccggccg acggcaccat
      421 aagaatcgga aatcgtcatc tcctcccgac tcccgactcc ggtaaacctc tcctcctcga
      481 gcatcgtcct ttcgtgaacg gaggtccctc atgagacggt ctctctccgc gatttcactg
      541 gccgccgctc ttctcctcgt cgcgtcctcg gcgcgcgccg tctactcgcc ggcgacgacg
      601 gcgggctggt tcatcacggc cgggcgcgtg cagggacagt tcggcgcgct cttccggact
      661 gacctttggc tcttcaatcc cgattcgtcg gcgagcgtga cgctgacgct gacgttccac
      721 cccgctgtcg gcgacggcgc gcctgccgcg ccgccgatca actccgcccc gatcgttctc
      781 gcgccgcggg agacgaagtt cttccccgac gtgacgttct cgacggtccc tgcaggcgac
      841 ggggtcgtgg gcgccctgcg ctggcagtcg agcgccccga ttcttggcgc ggggcgcatc
      901 tacacctcgg ctcccgaggg gacgttcggc ttcttcgtgc cggcgatccc gatcgccgaa
      961 tcgctcccga ggaaatcggc gacgtcggac gcggtgaacg tcctccagat cttcggcgtg
     1021 aactcggggg acgcgaactt ccggacgaac ctggacgtga cgaacacgtc ctccgtcgcg
     1081 ctgccgatcg aagtccgcgt catcgatccg gtgacgaccg agatctacgg cgggaccctc
     1141 aactccaccg tggccgcgaa ctctctgctg cggctgggcg ctgttctctc gactgtggga
     1201 gccccccagg tccccggact tcggatcacc gtcgcggtga aggaaacggc tccggcgttc
     1261 tcgagcgggg gcatcctcgc cgcggcttac acgctcgaca accggacgca ggacgcgttc
     1321 gcgttcgtcg gacagcggca gtaagggggt caggtctcca cttgccactt tgctccgcaa
     1381 ggcggcaagg acttccacgg gcgtcggccc gcccggcgac ccgtgaacac ctgagcccta
     1441 cggaaccggg tccgcccctc ccttcgcgaa cgggtggcag cgcgcgatgc gacccgcggt
     1501 caacaggaat ccccgcggga agccgtagcg gctgatcgct tcccgcccgt aggcggagca
     1561 ggttggttcg tatcggcacc gcacgattcc cgtcttcccg atcaccgcgc cgagcgtcgc
     1621 ccgataagca tcgatcgcga agacgcccgc ctttgcggag ctctgggcgt cggcgggccg
     1681 aagggagtct cccgccgcga accccacgag tgccgcgatc gcgagcgcaa cccgaaatcg
     1741 ccgatccgtc cggccgaacg cttcgggcgc cggctcggcg acgaacgttc ccgcgctatc
     1801 ggcgcccggc atacttcgag acacccggga ccagcagacg ctggccgtcg agcgccttca
     1861 cgatcgcgag gatcgcgatg aacgcgtaga gagcgagcac gaagaacatc agcgtcgcgt
     1921 agagacatcc gagcgccggc aggagggtcc cgacgagggt ggccacgagc gcgatcgcca
     1981 ccacggctcc gaagagcaga aggccgttgc gcgcgtgcca ccgaacctca ggatcgtcgc
     2041 gcgagaagag gagcggaatg acgccgagga aagcgaggta gcaaaggacg agcatcgcgc
     2101 gaggggaaga catccgctcg ttcgccatct gcgatcgaac gataacagca ggacccgacg
     2161 ggggagagct agggcgtgtt ggtcttcaac cggatcgacc cgttgaccgt ctcgcagcgg
     2221 agcgtctcgg cgccgccgtt gtacgttccg cgagcctctt tcgggccgta cttgccctcg
     2281 accgccagct cgaagtcccc ctcgatccgg ccgttgaccg tctcgagcga gtagcggacc
     2341 gaagagttct tgtcgaaggc gacgtccacg cttccgttga ccgtctctac ccgcgactcc
     2401 ttcatcgggc cgcggaagac gatccgaacg gagccgttca ccgtggtcgc gcgcaccggc
     2461 ccgaatacgt cgcgcagctc gatcgacccg ttgacggcgt cgcaggcgat cgaattgccg
     2521 agaccgatgg cgagcacttt cccgttgcag gtctcgagtc gcgcctgggc gagtgccgga
     2581 aggtgaatct cgtaatccac gtgagcgttt cgccacccga agtcgaggaa gccgaagagg
     2641 cgccgccgac gcgggttcac cgtctcgagg tagatgacgt cgccggactt tctcacccgg
     2701 atctcggtca gccgaagagt ttcctcggcc cgtccgccgc gcgcgctctt gacggcgcgg
     2761 accctcagat aggggcggtc ccaggcgtaa gcctcgatca tcccgttgac gttttcgacc
     2821 gagacccgcg aaaccccttc catcgagtac gctttttcga agacctgctc ctccgcggac
     2881 gcgacagtcg cgaagaggag caacccgacc aggaggagag ctcggcgcat tccggctaga
     2941 ttaacgtagg ccgatcaaaa aggttctcgg atgcccccgg cgtccacggg ggtaggatcg
     3001 gagcctccgg gccgttatgt cactgttccg ggtcgccgac tgccacctgc acttcgaggg
     3061 cgcacttccc cccgacaccc tcgaggcgct cgcgcgccgc gcggggcacc gtttcgtgga
     3121 tcgcggagtc ttcgaagcgg agcgcctggc tgtgcggagc cgaagcgagt ttcttctcct
     3181 gtaccgggag atttgcggcc tcttccgatc ccccgaagat ttcggggatg ccgcgatcgc
     3241 gatcgctcga tcggtggccg agggaggcgc ggcgtacgcc gagatctacg tctcgccgga
     3301 gatcgccggt cgattcgggc tggagccgac cgcgtgcctc gtcgcgatcg acgacggttt
     3361 ccgccgggcg gaggccgaag gcggtacgcc ctgtcggatc ctgctcgacg ccgtccgcca
     3421 gtggggaccc gaatcggccg acagggtgct cgatctccac gagaagacgc ggctcccctc
     3481 cgtcgtcggc ttcgggctcg ggggggacga gcttgccctg cccgccgcgg cctttgcggg
     3541 ggtctacgcc cgggcgcgcg ccctcgggct gcggacgtcg gtgcacgccg gcgagtgggg
     3601 cggcgtcgag tcgctcaccg aggcgctcga ccacctgcgg cccgaccggg tggaccacgg
     3661 aatcgccgcc gcgaacgacg cgcatctgct cgcgaggttc gcggaggagt cgacgcccct
     3721 ctgggtctcg ccgacgtcga acgtcgtcac cggcgccgtc caggacctcg ggga
//
LOCUS       DASWAV010000331         1713 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U2665
            k141_657117, whole genome shotgun sequence.
ACCESSION   DASWAV010000331 DASWAV010000000
VERSION     DASWAV010000331.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960316
            Sequence Read Archive: SRR10260007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 1713)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1713)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:47:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,929
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1713
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_657117"
                     /isolate="SMAG_U2665"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.36444 N 132.31889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            50..1378
                     /locus_tag="VF958_06810"
     CDS             50..1378
                     /locus_tag="VF958_06810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012375772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase-related protein"
                     /protein_id="HEX9148857.1"
                     /translation="MKLPIARVIDRVDASRQQLWSLEAEEARRRLLTTDPDEVLSISG
                     SFALVASEGERVCLARSLDRPLRYFLAKDSNGPVLIVAERIDEIRRVLEENGWLDQFH
                     PTYTRMVPAHHVTTLRLLGCPDPNPTYQRFFDPPREILPPDLDVIGGHYVGALYAEVR
                     RWLALQDPRAPIGVMFSGGIDSGAILLCLDRALRDAGDSPARLKAFTLSLDGHGDDLQ
                     QAREFLRRVDLEMLAEEIPVDSGTVDPLEAVEVIEDYKALDVECAAVNLALLRALRER
                     YPDWRLLVDGDGGDENLKDYPVEENRELTIVSVVNNRMLYQEGWGVDSIKHSLVYSGG
                     YSRSCVRTYACARRYGFTGFSPYTRPSVVAVAEAIPFAELTRGSTERLYALKGEIVRR
                     GVAAVAGFDMPVFAKRRFQHGAGSSDVAERLFTGGEARYRGHFVSLHARA"
     gene            1382..>1713
                     /locus_tag="VF958_06815"
     CDS             1382..>1713
                     /locus_tag="VF958_06815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007332370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HEX9148858.1"
                     /translation="MSLSAEDRRIRALRSSRNSVDPWGPLGALSEKERRPGGHLESTL
                     TVFLAGSECPFTCAFCDLWRNTLEGPTPRGALLEQLRRVLSQSRSGGFPSRIKLYNAS
                     NFFDPRAVP"
BASE COUNT          258 a          578 c          572 g          305 t
ORIGIN      
        1 atcttgctgc ttcctcggag gtcatccgtg ccggaataga atcggcggcg tgaaactccc
       61 gatcgctcgc gtgatcgacc gcgtggacgc gagccgacag cagctctggt ccctcgaggc
      121 cgaggaagcc cgccgaaggc tgttgacgac cgacccggac gaagtcctct cgatctcggg
      181 ctcgttcgcg ctcgtcgcga gcgagggtga gagggtctgc ctggctcgga gtctcgatcg
      241 gccgctgcgc tacttcctcg cgaaggattc caacggtccg gtgctcatcg tcgccgagcg
      301 gatcgacgag atccgccgcg tgctcgagga aaacgggtgg ctggaccagt tccatccgac
      361 ctacacccgg atggtccccg cgcaccatgt gacgacgctt cgtctcctcg ggtgcccgga
      421 tccgaacccg acgtatcagc gcttcttcga tccgccgcgc gaaattcttc cacccgacct
      481 ggacgtgatc ggcgggcatt acgtgggagc gctctacgct gaggtccgac gttggcttgc
      541 gctgcaggac ccgcgcgctc cgatcggcgt gatgttcagc ggcggaatcg acagcggcgc
      601 gatcctgctc tgtctcgacc gggcgctgcg cgacgcgggc gacagcccgg cgcggctcaa
      661 ggccttcacc ctctcgctcg acggccacgg cgacgacctc cagcaggcgc gcgagttcct
      721 tcgccgcgtc gacctcgaga tgctcgcgga agagatcccg gtcgactcgg ggaccgtcga
      781 tcccctcgag gcggtcgaag tgatcgagga ctacaaggct ctggacgtgg aatgcgccgc
      841 ggtcaacctc gctttgctcc gcgcgcttcg agagcgttac cccgactggc gtctcctcgt
      901 ggatggagac gggggggacg agaacctgaa ggactatccg gtcgaggaga accgcgagct
      961 cacgatcgtg agcgtcgtga acaaccggat gctgtaccag gaagggtggg gggtcgactc
     1021 gatcaagcac tcgctcgtct acagcggcgg ctacagccgg tcgtgcgtgc ggacgtacgc
     1081 gtgcgcgcgc cggtacggct tcacgggatt cagcccgtac acgcgtcctt ccgtcgtcgc
     1141 ggtcgcggaa gcgatcccgt tcgcggagtt gacccgcggc tcgaccgagc ggctctatgc
     1201 cctcaagggc gagatcgtgc gccgtggcgt cgccgcggtc gccggctttg acatgcccgt
     1261 cttcgccaag cggcgatttc aacatggcgc gggatcctcc gacgtggccg aacgtctctt
     1321 caccggaggg gaagcgcgtt accggggcca cttcgtctcc cttcacgcac gggcctaggc
     1381 cgtgagcctc tccgccgagg accggcggat ccgggccctc cgctcttccc gtaactccgt
     1441 cgatccctgg gggccgctcg gcgcgctctc ggagaaggag cgtcggccgg gcggccacct
     1501 cgagagcacg ctgacggtct tcctcgcagg gagcgagtgc cctttcacgt gcgcgttttg
     1561 tgacctctgg cgaaacactc tcgaaggtcc gactccccgg ggcgctctgc tggagcagct
     1621 gcgtcgggtg ctctcgcagt cgcgctccgg cgggtttccg agccgcatca agttgtataa
     1681 cgcgagcaat ttcttcgacc cgcgggcggt gcc
//
LOCUS       DASWAV010000332         1678 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U2665
            k141_297573, whole genome shotgun sequence.
ACCESSION   DASWAV010000332 DASWAV010000000
VERSION     DASWAV010000332.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960316
            Sequence Read Archive: SRR10260007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 1678)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1678)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:47:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,929
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1678
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_297573"
                     /isolate="SMAG_U2665"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.36444 N 132.31889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(159..1544)
                     /locus_tag="VF958_06820"
     CDS             complement(159..1544)
                     /locus_tag="VF958_06820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011423163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M64 family metallopeptidase"
                     /protein_id="HEX9148859.1"
                     /translation="MLVAPVVVCAAPARTMRLDYYHTGTAAGEQFSVDRIVIEPLPWA
                     GGTAIDDTNLGKYLFEVIDRATNRPVYSRGFASIYGEWEVTAEARSAARTFHESLRFP
                     APDGPVQVVLKKRDAANAFREIWSVLVDPKDMFVDSSKPASPGPILEIQKSGDPAEKV
                     DFLIIGDGYTAAERSKFEKDARRLTEILFSFAPFKEHRSDFNVWGLCPPSEESGISRP
                     STGVHRRSRVGATYDAFGSERYLLSFENRLLRDVASFAPYDFIEILTNTRTYGGGGIF
                     GLFSTVAADSFWSPYVFVHEFGHHFAGLADEYYTSDVAYEPAAERAEPWEPNATALLD
                     PANLKWKDLVSSGTPIPTRWNKDAFEALSREIQKRRRQIRAENRPEEEMDSLFREQKG
                     KETKMLAAEPYAGKVGAFEGAMYEAKGYYRPQVDCIMFSRNDVGFCAVCRRAIERVIA
                     LYTSPRAPAAR"
BASE COUNT          291 a          543 c          559 g          285 t
ORIGIN      
        1 gttgcagagg ttcgtgaccc gcagctgcac gaacgtcggc atcggcaggt tcccggcggt
       61 cggtgacgcg aggtgttcgc ggacgccgcg cttcccgcgg ttcattcgcg ccaggtaccg
      121 cgaagcccag ccggaaagcg cgagcccgcg gggaaagctc atcgcgccgc gggagcgcgg
      181 ggggatgtat agagcgcgat cacgcgctcg atcgcgcgcc ggcagaccgc gcagaatccg
      241 acgtcgttgc gcgagaacat gatgcaatcc acctgcggac ggtagtagcc cttcgcctcg
      301 tacatcgccc cttcgaacgc accgaccttt ccggcgtacg gctccgccgc gagcatcttc
      361 gtctccttgc ccttctgctc gcggaagagc gagtccatct cctcttcggg gcggttctcg
      421 gcgcggatct gccggcggcg tttctggatt tcgcgcgaca gcgcctcgaa agcgtccttg
      481 ttccaccgcg tggggatcgg cgtcccggac gacaccaggt ctttccactt gaggttcgcc
      541 ggatcgagaa gagcggtcgc gttcggctcc cacggttcgg cgcgctcggc ggcgggctcg
      601 tacgccacgt cggacgtgta gtactcgtcg gcgagcccgg cgaagtggtg accgaactcg
      661 tgcacgaaca cgtacggcga ccagaagctg tcggcggcga ccgtcgagaa gaggccgaaa
      721 atcccgccgc cgccgtacgt ccgcgtgttc gtcaggatct cgatgaaatc gtacggcgcg
      781 aaggaggcca cgtcgcgcag gagccggttc tcgaacgaga ggaggtaacg ctccgagccg
      841 aacgcgtcgt aggtcgcgcc gacgcgcgag cgccggtgca ctccggtcga cggccgcgag
      901 atcccggact cttccgacgg ggggcagaga ccccacacgt tgaagtcgga ccgatgctcc
      961 ttgaagggcg cgaacgagaa cagaatctca gtcaggcggc gcgcgtcctt ctcgaacttg
     1021 gaccgctcgg ccgccgtgta tccgtcgccg atgatcagga agtcgacctt ctccgccgga
     1081 tcgccggact tctggatctc gagaatcggt ccgggcgaag ccggtttcga ggaatcgacg
     1141 aacatgtctt tcggatcgac gagcacggac cagatctcgc gaaacgcgtt cgcggcgtct
     1201 cgtttcttca gcacgacctg aaccggcccg tcgggcgcgg ggaatcggag tgactcgtgg
     1261 aaagtgcgag ccgccgacct ggcctcggcg gtcacttccc actcaccgta aatcgaggcg
     1321 aacccgcgcg agtagaccgg ccggttggtc gcgcggtcga tgacctcgaa caagtacttg
     1381 ccgaggttgg tgtcgtcgat cgcggttccg ccggcccacg ggagtggctc gatcacgatg
     1441 cggtcgacgc tgaactgctc ccccgcggcg gttcccgtgt ggtagtagtc gagtctcatc
     1501 gtgcgcgccg gcgcggcgca cacgaccacc ggcgccacga gcatggcggc ccagagaagg
     1561 actccacgag gtttcacttt gcgaatatcc tacgtcctcg gggtcaggtc tgatagagcg
     1621 agtcagaccc aatgtggcaa gtggagacct gacccccaaa ggagtctcat gattcacg
//
LOCUS       DASWAV010000333         3417 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U2665
            k141_217555, whole genome shotgun sequence.
ACCESSION   DASWAV010000333 DASWAV010000000
VERSION     DASWAV010000333.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960316
            Sequence Read Archive: SRR10260007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 3417)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3417)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:47:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,929
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3417
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_217555"
                     /isolate="SMAG_U2665"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.36444 N 132.31889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..301)
                     /locus_tag="VF958_06825"
     CDS             complement(<1..301)
                     /locus_tag="VF958_06825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013953962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HEX9148860.1"
                     /translation="MTNVFPSVRSGPGLLEAIGSTPHAELSRFSAGAARIFAKLEFLN
                     PGGSVKDRIALSMIEDAERRGLLRPGGTIVEPTSGNTGVALAMIAAVKGYHCIVVM"
     gene            complement(372..671)
                     /locus_tag="VF958_06830"
     CDS             complement(372..671)
                     /locus_tag="VF958_06830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013402841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-ribbon domain containing protein"
                     /protein_id="HEX9148861.1"
                     /translation="MMQQYTDKNLTCVDCGNTFLFSADDQEFYARKGFTNEPKRCKVC
                     RDKRKAAAGEGRERTLYDVTCDGCGKQTQVPFNPTNGKPVYCRECYSARRSQQAY"
     gene            complement(1069..1890)
                     /locus_tag="VF958_06835"
     CDS             complement(1069..1890)
                     /locus_tag="VF958_06835"
                     /EC_number="2.8.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012646674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1990107 - thiazole synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole synthase"
                     /protein_id="HEX9148862.1"
                     /translation="MSTAAPTETVPTPGGQSAPDPFVLGGRTFTSRLIIGTGKYATFP
                     LMKEAHRESGAEIVTVAVRRINLDRTKESLLDFIPPNMTLLPNTAGCYTADDAIRTAR
                     LGREVGLSNWVKLEVLGDERTLWPDNAGLLEATRTLVAEGFVVFPYTSDDPVVCRRLE
                     DEGAAAVMPLGAPIGSGLGIQNPNNISIIIEHAKVPVIVDAGVGTASDACIAMELGAA
                     GVLMNTGIAGASDPIRMARAMRRAVAAGRDAFLAGRIPRKRYATASSPIEGLLKP"
     gene            complement(1922..2761)
                     /gene="panC"
                     /locus_tag="VF958_06840"
     CDS             complement(1922..2761)
                     /gene="panC"
                     /locus_tag="VF958_06840"
                     /EC_number="6.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010161803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004592 - pantoate-beta-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HEX9148863.1"
                     /translation="MKVVREVSALRAIVGEARESGKSIAFVPTMGALHEGHLSLARLA
                     RREAGFVVASIFVNPLQFAPTEDLSRYPRREEADSAAFARENVDVVYAPEPAVMYPPG
                     FSTTVEVTGVTEGGEGAARPGHFRGVTTVVAKLFLQVAPDVAVFGRKDLQQVAVIRRM
                     VRDLDFPIRLLVGETVREPDGLAMSSRNAYLSPEDRRLASVFPRALSRARDAAARGER
                     DAAKLAADVRRELEAAGMQVDYVEVVDPETMRPVEKAISGAAVTAAIRIGKTRLIDNV
                     LLP"
     gene            complement(2758..>3417)
                     /gene="panB"
                     /locus_tag="VF958_06845"
     CDS             complement(2758..>3417)
                     /gene="panB"
                     /locus_tag="VF958_06845"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013174340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003864 - 3-methyl-2-oxobutanoate
                     hydroxymethyltransferase activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HEX9148864.1"
                     /translation="SATMDSMVRHSRAVSRAAKRALVVGDMPFLSYQADVSRAVENAG
                     RFLAEGGCAAVKLEGGSRILPAVEAILAADIPVMGHVGLTPQSYHKFGGFKVQGREPA
                     TAREIVEDAKALAAAGCFSIVLECVPAALAAEITREVPVPTIGIGAGAECDGQVLVFH
                     DVMGLTRDLRPRFVRKYADLSAVIEEAAKAFARDVRDGSFPTEAESFSGARPTALRRV
                     Y"
BASE COUNT          585 a         1108 c         1167 g          557 t
ORIGIN      
        1 gcatgacgac gatgcagtga tatcccttca ccgcagcgat catcgcgagc gcaaccccag
       61 tgtttcccga agtgggctcc acgatcgtgc cacccggccg cagcagcccg cgcctctcgg
      121 cgtcctcgat catggagagg gcgatccggt ccttgacgct tccacccggg ttcaggaact
      181 cgagtttcgc gaagatccgg gccgcgccgg ccgaaaaccg cgagagctcc gcatgaggcg
      241 tggagccgat cgcttcgaga agtccggggc cggagcgaac ggaagggaag acgtttgtca
      301 cgatagccaa cgaaaccaca aaaaagcccc ggcattgccg gggctttcga tgaacgtgaa
      361 aaaactccgg ctcagtaggc ctgctgcgag cggcgtgccg aatagcactc acggcagtag
      421 acgggcttgc cgttcgtcgg gttgaagggg acctgtgtct gcttgccgca gccgtcgcag
      481 gtcacgtcgt agagcgtgcg ctcccgtccc tcaccggcgg ccgccttgcg cttgtcgcgg
      541 cacaccttgc accgcttcgg ctcgttggtg aatcccttgc gggcgtagaa ctcctggtcg
      601 tccgccgaaa aaaggaacgt gttaccgcaa tcaacgcagg tcagattctt gtccgtgtat
      661 tgctgcatca ttgaaattgc cgatctcctg ggtattaccc tcttccgaag cgttcatgcg
      721 cttcgttgat tcaaaaaaaa actgatagaa actcgtgtat tcgaaaaggc tccggcggta
      781 gccggaaaga cttcttccct tagtaccgac cccctttcga gcgcgcgtac gcctccccgc
      841 gagagcggac ggatggggga tgagaaaaca tagtgtggtg gccgggtaat cttttgcggg
      901 gcgtttctta aagttgacgc aaagtagcac gcgctcgaac ggcttggcaa ggcaaatttg
      961 ccgcggcatg gtcgtacgac caaagtcgaa tgcgggccga aaagcctcgg aatcgcgaag
     1021 ttttaccgtc tgccgccggg agagggtgcc aggggtgagg gggcttggtt acggcttgag
     1081 gagcccttcg atcggagacg acgcggtcgc gtagcgcttg cgcggaatgc gaccggcgag
     1141 gaaggcgtca cgcccggcgg cgaccgcgcg ccgcatcgcg cgcgccatcc gaatcggatc
     1201 gctcgcgccc gcgatcccgg tgttcatgag cacgccggcg gcgccgagct ccatagcgat
     1261 gcacgcgtcg gacgccgttc ccactcccgc gtcgacgatc accggcacct ttgcgtgctc
     1321 gatgatgatc gagatgttgt tcgggttctg gatgccgagc ccggagccga tgggcgcacc
     1381 gagaggcatc acggcggccg caccttcgtc ctcgaggcgc cggcacacca ccggatcgtc
     1441 cgaggtgtag gggaacacga cgaatccctc ggcgacgaga gtgcgcgtcg cctcgagcag
     1501 ccccgcattg tcgggccaga gagtgcgctc gtcgccgagc acctcgagct tcacccagtt
     1561 ggacaggccg acttcgcggc cgagccgcgc cgtgcggatg gcgtcgtcgg ccgtgtagca
     1621 gccggcggtg ttcggaagga gcgtcatgtt cggggggatg aagtcgagga gcgactcttt
     1681 cgttcggtcg aggttgatgc gccgcaccgc gacggtgacg atctcggcgc cggactcgcg
     1741 gtgcgcctcc ttcatcaacg gaaaagttgc gtacttgccc gtgccgatga tcagccggga
     1801 agtgaaggtt cgccctccga gtacgaaggg gtccggcgcg gactgcccgc cgggggtcgg
     1861 gaccgtttcg gtcggcgctg cggtgctcac tcagtgatcc tgtcgcgttg cgcgggcaag
     1921 gtcaaggaag gaggacgttg tcgatgaggc gcgtctttcc gatccgaatg gcggccgtga
     1981 ccgcggcgcc ggaaatcgcc ttctcgacgg gcctcatggt ctccggatcg acgacctcca
     2041 cgtagtccac ctgcatcccc gccgcctcga gctcccgccg gacgtctgcg gcgagtttcg
     2101 cggcgtcgcg ctccccccgc gcggcggcgt cgcgtgctcg cgaaagagcc cgggggaata
     2161 cggaggcgag gcgtcgatcc tccggtgaga gataggcgtt gcgcgaagac atcgcgaggc
     2221 cgtccggctc gcgcacggtc tcgccgacga ggaggcggat cgggaaatcg agatcgcgga
     2281 ccatgcggcg gatgacggcg acctgctgga ggtctttgcg tccgaagacg gcgacgtccg
     2341 gcgcgacctg caggaagagc ttcgcgacga cggtcgttac accgcggaag tggccgggcc
     2401 gcgcggcgcc ttcgcccccc tccgtcacgc cggtcacctc gacggtggtc gagaagcccg
     2461 ggggatacat gaccgccggc tccggcgcgt agacgacgtc gacgttttcg cgcgcgaatg
     2521 ccgccgagtc ggcctcttcc cggcgcgggt agcgcgagag gtcctcggtc ggcgcgaact
     2581 ggagcgggtt gacgaagatg gacgccacga cgaaaccggc ctcgcgtcgc gcgagacgcg
     2641 cgagcgagag gtgtccctcg tggagagcgc ccatcgtcgg gacgaaggcg atggacttcc
     2701 cggactcgcg cgcctcgccg acgatcgccc ggagcgcgga gacctcccgg acgaccttca
     2761 gtagacccgc cgaagggccg tcggcctggc gccggagaag gactccgctt ccgtcggaaa
     2821 gctgccgtcc ctgacgtccc tcgcgaacgc cttcgcggcc tcctcgatca ccgccgacag
     2881 gtccgcgtac ttgcgcacga aacgcgggcg caggtcgcgc gtcaaaccca tcacgtcgtg
     2941 gaagacgagg acctggccgt cgcattcggc gcccgcgccg atgccgatcg tcggcacggg
     3001 aacttctcgc gtgatttcgg cggcgagcgc cgcgggcacg cactcgagca cgatcgagaa
     3061 gcagccggcg gccgcgagag ccttcgcgtc ctcgacgatc tcacgcgccg tcgccggctc
     3121 gcgcccctgg accttgaagc cgccgaactt gtggtacgac tgcggggtca ggccgacgtg
     3181 acccatcacc gggatgtcgg ctgcgaggat cgcctcgacc gcggggagga tccggcttcc
     3241 accctcgagc ttcaccgcgg cacagccccc ttccgccagg aaacggccgg cgttctcgac
     3301 ggcgcgggag acgtccgcct ggtaagaaag aaacggcatg tcgccgacga cgagcgcgcg
     3361 tttggccgcc cgcgagacgg cgcgcgagtg gcggaccatc gagtccatcg tggcgga
//
LOCUS       DASWAV010000334         2057 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U2665
            k141_97728, whole genome shotgun sequence.
ACCESSION   DASWAV010000334 DASWAV010000000
VERSION     DASWAV010000334.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960316
            Sequence Read Archive: SRR10260007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2057)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2057)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:47:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,929
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2057
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_97728"
                     /isolate="SMAG_U2665"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.36444 N 132.31889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..273)
                     /locus_tag="VF958_06850"
     CDS             complement(<1..273)
                     /locus_tag="VF958_06850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012924292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX9148865.1"
                     /translation="MTARPRALVTGASAGIGAAFAERLAREGYDLVVVARRRERLEEL
                     AQRLKGVGKTAVDVLIADLTQGEQLRMVERRISEDSALEMLVNNAGF"
     gene            complement(270..491)
                     /locus_tag="VF958_06855"
     CDS             complement(270..491)
                     /locus_tag="VF958_06855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9148866.1"
                     /translation="MPDLCSFHPSIWSGFPGRLGDPMPPQPEVRRRDSAEPNPAPLVV
                     SAADPLNLPGILTPDGRVAPGSSRQVRVA"
     gene            778..1773
                     /locus_tag="VF958_06860"
     CDS             778..1773
                     /locus_tag="VF958_06860"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEX9148867.1"
                     /translation="MRIWVHDPEAPSFRYRLEAAYPALERHGFSCETERFPRRRYGLR
                     VLERRKDLKRLDLLVVAKLKLLPGETTLVRASARRIVYDFDDAVYFAKPKRPGEAPDR
                     SAFRIRKFAATCRMADLVVAANGELAAFARRWARRVEVVPTGVDVTRYLPAGSPRSSR
                     SALVWIGLPGNLSYLELVRPAIGVLAREFPGLSLRIICSRFPDWPEVPIERVAWTEES
                     APAALASASIGIMPLSDDEWTRGKRGFKLLQYMAARLPCVASPVGSNREIIVEGETGY
                     LPADDRGWLDAIRTLLGSENRRQAMGEAGRVKVERLYSMESIAERTADLYEKLVV"
     gene            complement(1804..>2057)
                     /locus_tag="VF958_06865"
     CDS             complement(1804..>2057)
                     /locus_tag="VF958_06865"
                     /inference="COORDINATES: protein motif:HMM:NF013283.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="BamA/TamA family outer membrane protein"
                     /protein_id="HEX9148868.1"
                     /translation="WKKPGDIRVALEGSDGLRQGVGVGLRYMTPIGPVRVEYGWPLDA
                     HTVPFNIQLLTDKGAILDTIPAGTRKEKGQFFFSIGYPF"
BASE COUNT          327 a          699 c          692 g          339 t
ORIGIN      
        1 gaaccccgcg ttgttgacga gcatttcgag tgcggagtct tccgagattc tcctctctac
       61 catccgcagc tgttcaccct gtgtcagatc cgctatgagg acgtcgaccg ccgtttttcc
      121 gacgcccttc agccgctgcg cgagctcctc caggcgttcg cgcctccgcg cgacgacgac
      181 gaggtcgtac ccctcgcggg cgagcctctc cgcgaacgcc gcgccgattc cggcagacgc
      241 cccggtgacg agagcgcgcg ggcgggcggt cacgcgaccc gcacctgccg gctcgagccc
      301 ggcgcgacgc ggccgtcggg ggtcaggatt cccggcaagt tcagcgggtc ggccgccgag
      361 accacgagcg gagccggatt cggctccgcg gagtcgcgcc ggcgcacctc gggctgcggg
      421 ggcatcgggt caccgaggcg gccgggaaac ccgctccaga tcgaggggtg aaaggagcag
      481 aggtcaggca ccggaccgga tactaacggc aacgaccggc gatgccgaag tgggaacgcg
      541 cgcgccgtcc gaagcgtttc gagtgaggac gatgcgaccg cggaacaccg tgttgggcgg
      601 ttcagatagc cggggacccc ggcagggagc gttcggacga cggaaacgaa aagtccggac
      661 cccacggagc ggaaaaggcg gtagcgattc aatggggtga gtcagccggc gacgtcggtg
      721 ccatcgactc ctcccccgcg ccgggacgct gacgggagac ccgctttcct gcggcgaatg
      781 agaatctggg tccatgaccc ggaggcgccg tcttttcgct atcgtctcga ggccgcgtac
      841 cccgcgctcg agcggcacgg tttctcctgc gagaccgaaa ggttcccccg gcgccggtac
      901 ggcctgcgcg tgctcgagcg acggaaggac ttgaagagac tcgaccttct cgttgtcgcg
      961 aagctgaagc tcctccccgg agagaccacg ctcgtccgcg cgagcgcgcg gcggatcgtg
     1021 tacgacttcg acgacgcggt ctacttcgcg aagccgaagc ggccgggcga ggcacccgat
     1081 cgctcggcgt tccggatccg caagttcgcc gcgacgtgcc gcatggccga cctggtcgtc
     1141 gccgccaacg gcgaactcgc cgcgtttgcc cggcgttggg cgcgccgggt cgaggtcgtc
     1201 ccgaccgggg tggacgtcac ccgctacctg cctgccggga gtccccgctc aagccgctcg
     1261 gccctcgtct ggatcggcct gccggggaat ctctcgtacc tggagctcgt ccgtccggcg
     1321 atcggcgtgc tcgcgcgcga gttcccgggc ctctcccttc ggatcatctg ttcccgcttc
     1381 ccggattggc ccgaggtccc gatcgaacgg gtcgcctgga cggaagaatc tgccccggcc
     1441 gcgctcgcgt cggcttcgat cggaatcatg cccctttccg acgacgagtg gacgcgcgga
     1501 aagcgcggct tcaagcttct ccagtacatg gcggcgcgtc tcccgtgtgt cgcgtcgccg
     1561 gtcggcagca atcgggagat catcgtcgag ggcgagacgg gctaccttcc cgcggacgat
     1621 cgcggctggc tcgacgcgat tcggaccctc ctcggctcgg aaaatcgccg gcaagcgatg
     1681 ggagaggccg gtcgcgtcaa agtcgaacgg ctctattcga tggagagcat cgcggagcga
     1741 actgccgacc tgtacgagaa gttggtcgtt tgatcatctg ctcatttctt cgcgagcggc
     1801 cgctcagaac ggatagccga tcgagaagaa gaactgccct ttctcctttc gcgtgccggc
     1861 cgggatggtg tcgagaatgg ctcccttgtc ggtcagcagc tgtatgttga acggaaccgt
     1921 gtgcgcgtcc agaggccacc cgtactccac gcgcaccgga ccgatgggcg tcatgtaacg
     1981 gagcccgacc ccgacgccct ggcgcaatcc gtcgcttcct tccaacgcga cgcgaatgtc
     2041 gcccggcttc ttccaca
//
LOCUS       DASWAV010000335         2573 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U2665
            k141_97805, whole genome shotgun sequence.
ACCESSION   DASWAV010000335 DASWAV010000000
VERSION     DASWAV010000335.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960316
            Sequence Read Archive: SRR10260007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2573)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2573)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:47:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,929
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2573
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_97805"
                     /isolate="SMAG_U2665"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.36444 N 132.31889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..465)
                     /locus_tag="VF958_06870"
     CDS             complement(<1..465)
                     /locus_tag="VF958_06870"
                     /inference="COORDINATES: protein motif:HMM:NF012382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEX9148869.1"
                     /translation="MTRPRLSRRAAGIVPSSTAAVARKARALAASGVDVVDFGIGEPD
                     FATPEFVAAAGMAAIEEGRTRYTDVAGEGALRDAIAEKYRREYGVPVARENVLVTAGA
                     KQAVFNACQVLFEAGDEVALFCPYWVSFPEIVRLSGASPLLVPTELETRWKPV"
     gene            complement(462..1661)
                     /locus_tag="VF958_06875"
     CDS             complement(462..1661)
                     /locus_tag="VF958_06875"
                     /inference="COORDINATES: protein motif:HMM:NF013832.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9148870.1"
                     /translation="MKSWRERLTLPAAIASEAAGSPASAVWTFPLMIFAAFVVSWGAE
                     AAEFLISQGLALAILALIQTLPEFAVEAVIAWKAGKDPNMVHLAIANFTGSLRLLTGL
                     GWPMIYAVAAFYSRRKHGRKLGSIDLHDDHAVEVMGLLPPLAWFIVVWAKSSLSLVDA
                     FVLTTTYVVYLVVLLKLPPRDETVEEDEEIPRVSKWALSQTGWKRWAAVLGLLVAGGA
                     IIFFVAEPFLASMMALAATMGISQFVFIQWVAPFLSEFPEKTSAFAWARRVTRAPVAL
                     MNMVSSNINQWGVLSAMLAVIYCVSKGEVTALPFDEFQRLEILLTIVQAFLGWIFLAS
                     MSLSAYEAAGLFVLWVIQFVRPSLRKEMLWVYGAWIVFELVRIVIGKQKIRAFGALAR
                     VWKRRKR"
     gene            1719..2486
                     /locus_tag="VF958_06880"
     CDS             1719..2486
                     /locus_tag="VF958_06880"
                     /inference="COORDINATES: protein motif:HMM:NF012797.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrmH family RNA methyltransferase"
                     /protein_id="HEX9148871.1"
                     /translation="MTAIDSPSNPLIRQISRSLEERTHLLLEGEKTILDAVAAGALLD
                     HVLHDESVRPGRLATLTSTRPRLVSRKVLERLADSQTPQHLIAIARRRDVPSADILGR
                     PGVVVFVFGIQDPGNLGAIVRVAEAAGAAGVVAAPGTADFFHPRAVRGSAGSVLRVPV
                     SGKVSFEPFAASAREAGRSICGAVSEGGDDPFEKPVPEKSVLVIGAEGAGLPAGAYRY
                     LDRRITIPMRPPVDSLNAAVAAALLLYSTRLAGESRE"
BASE COUNT          441 a          901 c          858 g          373 t
ORIGIN      
        1 gaccggcttc cagcgggtct cgagctcggt cgggacgagg agcggcgagg cgccggagag
       61 ccggacgatc tcggggaacg acacccagta gggacagaag agggcgacct cgtcgccggc
      121 ctcgaagagc acctggcagg cattgaagac cgcctgcttc gccccggccg tcacgagcac
      181 gttctcgcgg gctacgggga cgccgtattc ccgccggtac ttctccgcga tcgcatcccg
      241 cagcgctccc tctccggcga cgtcggtgta gcgcgtccgc ccctcctcga ttgcggccat
      301 gcccgccgcc gccacgaact ccggcgtcgc gaaatccggc tcgccgatcc cgaagtcgac
      361 gacgtcgacg cccgaagcgg cgagcgcccg ggcctttcgc gcgacggcgg ccgtggagga
      421 aggaacgatc ccggcggcgc ggcgggagag gcgcggccgg gtcatcgctt cctcctcttc
      481 cagacccgcg ccagggcgcc gaacgcccgg atcttctgtt ttccgatcac gatccgcacg
      541 agctcgaaca cgatccatgc accgtacacc cagagcatct cttttcgcag cgacggacgc
      601 acgaactgga tcacccagag aacgaaaagg ccggccgcct cgtaggcgga aaggctcatc
      661 gacgcgagaa agatccagcc gaggaacgcc tgcacgatcg tcaggaggat ctccaggcgc
      721 tggaactcgt cgaagggcag cgccgtcacc tcgcccttgc tcacgcagta gatgacggcg
      781 agcatcgccg agagcacccc ccactggttg atgttcgacg acaccatgtt catgagggcc
      841 acgggcgccc gcgtgacccg ccgcgcccag gcgaaggcgg acgtcttctc ggggaactcc
      901 gacaggaacg gcgcgaccca ctggatgaag acgaactggg agattcccat cgtggccgca
      961 agagccatca tggaggcgag gaacggttcg gcgacgaaga agatgatcgc gccgccggcc
     1021 acgagaagac ccaacaccgc agcccacctc ttccagccgg tctgggagag cgcccacttg
     1081 gagactctcg ggatctcctc gtcctcctcg acggtttcgt cccgaggcgg cagcttcagc
     1141 agcacgacga ggtaaacgac gtaggtcgtg gtcagcacga acgcgtcgac gagagagagg
     1201 ctgctcttcg cccagacgac gatgaaccag gcgagtgggg gcaggaggcc catgacctcg
     1261 accgcgtggt cgtcgtgaag gtcgatcgag ccgagctttc gtccgtgctt gcggcgcgag
     1321 tagaaagccg cgaccgcgta gatcatgggc cagccgaggc ccgtcaggag gcgaaggctt
     1381 cccgtgaagt tggcgatcgc gaggtggacc atgttggggt ccttccccgc tttccacgcg
     1441 atgaccgctt cgacggcgaa ctcgggcagc gtctggatga gcgccaggat cgcgagagcc
     1501 aggccctgcg agatgaggaa ctccgccgcc tccgctcccc acgacaccac aaaggccgcg
     1561 aagatcatca gcgggaacgt ccagacagcg gaggccgggg aacccgcggc ttcggacgcg
     1621 atggcggccg gcagggtgag ccgctcgcgc cacgatttca tcgcgccggt ttatagcacg
     1681 cgcgtccgat cgatcggcaa tcgcgaaata atgagcgcgt gacggcgatc gactccccgt
     1741 cgaatccctt gatccggcag atctctcgtt cgctcgagga acggacgcat ctcctcctcg
     1801 agggagagaa gacgatcctg gacgcggtcg ccgccggcgc gctcctcgat cacgtcctgc
     1861 acgacgagag cgtgagaccc ggccgcctgg cgacgctgac gtcgacgcgc ccgcggctcg
     1921 tctcgcgcaa ggtcctcgag aggctcgcgg attcgcagac tccgcaacac ctgatcgcga
     1981 tcgcgcggcg gcgcgacgtc ccctccgccg acatcctcgg ccgcccgggc gtcgtcgtgt
     2041 tcgtcttcgg gatccaggat ccgggcaacc tcggagcgat cgtgcgcgtg gcggaagcgg
     2101 ccggcgcggc gggagtcgtc gcggcgcccg gcaccgccga cttcttccac ccgcgcgccg
     2161 tccggggaag cgcggggagc gtcctgcgcg tgcccgtctc cggaaaagtc tcgttcgagc
     2221 cgttcgccgc gtccgcgcgg gaggccggcc gctccatctg cggcgcggtg tccgaggggg
     2281 gcgacgaccc tttcgagaag ccggtccccg agaagtctgt cctggtcatc ggcgcggagg
     2341 gcgccggcct cccggccggc gcttaccgct acctcgaccg gcggatcacg atccccatgc
     2401 ggccgccggt cgattccctg aacgcagcgg tcgccgccgc gctgctgctc tactccaccc
     2461 gcctcgcggg tgaaagccgc gaatagcgcg ctacttcttc gccatcgaca tcgtgccttc
     2521 ccaggcgatg cgcgcgaagt tgccgggcgc cgcagcgacg ctgttcttcc acg
//
LOCUS       DASWAV010000336         6647 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U2665
            k141_575358, whole genome shotgun sequence.
ACCESSION   DASWAV010000336 DASWAV010000000
VERSION     DASWAV010000336.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960316
            Sequence Read Archive: SRR10260007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 6647)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6647)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:47:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,929
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6647
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_575358"
                     /isolate="SMAG_U2665"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.36444 N 132.31889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..619)
                     /locus_tag="VF958_06885"
     CDS             complement(<1..619)
                     /locus_tag="VF958_06885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015347803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9148872.1"
                     /translation="MAFIDFASREIHCKVVYYGPGLGGKTANLRYLSEHARPELRGRL
                     ISLSTDTDRTLFFDFLPVDLAPLRGFRPRFNLYTVPGQVFYDAARRAVLRGADGIIFV
                     ADSQEARLHANVESIRNLDAHLKSYDRDLAEIPYVLQLNKRDLPSALPAEELWRRLNI
                     KGEPTIEATAPSGEGVLETLKIAAREVLMDLRRRPVPRVIQPTLAT"
     gene            complement(718..1215)
                     /locus_tag="VF958_06890"
     CDS             complement(718..1215)
                     /locus_tag="VF958_06890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005034515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="roadblock/LC7 domain-containing protein"
                     /protein_id="HEX9148873.1"
                     /translation="MTPADLVLYDEDFRRLQQILRRLRQEADAMIVLLVDKNGQRIAS
                     AGDVDALDVTALASLTAGNVAATDGLARILGEREFSVLLHEGEKQNIHLSVVGGKVIL
                     VVIFADRSSLGLVRLRVRQAAQELAQAFAEINERMQRAPVLHSAFEPLLAEITDEDID
                     RLFSE"
     gene            complement(1254..1844)
                     /gene="recR"
                     /locus_tag="VF958_06895"
     CDS             complement(1254..1844)
                     /gene="recR"
                     /locus_tag="VF958_06895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012282270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination mediator RecR"
                     /protein_id="HEX9148874.1"
                     /translation="MKLKGLDRLVSEFQRLPGIGAKSASRLAYYLLRSPRDEAERLAS
                     AITESRDAVRRCSVCNTLTEDDPCEICSDPQRDASLLCVVEEPGNVAAIEKTREFRGR
                     YHVLWGALSPLRGIGPDEIDIGGLVARVTPEIREVVLATNPNVEGETTALYIAKRLKT
                     SGVRVTRIAFGLPVGGDIEYADEVTMARSLENRREL"
     gene            complement(1846..2142)
                     /locus_tag="VF958_06900"
     CDS             complement(1846..2142)
                     /locus_tag="VF958_06900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020721491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HEX9148875.1"
                     /translation="MNIQKMMQQAQAMQSKVQAELEAMRLQGSAGGGLVSIEMSGAKE
                     VLAVKIDPSAVNREDVETLEDLVLAALRDATRRVDEAVQKKTSSMLGGMLPGMR"
     gene            complement(2158..3825)
                     /gene="dnaX"
                     /locus_tag="VF958_06905"
     CDS             complement(2158..3825)
                     /gene="dnaX"
                     /locus_tag="VF958_06905"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF004046.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HEX9148876.1"
                     /translation="MAHLSLPRKWRPRTFADLIGQDSVARTLMNAISEVRIAPAYLLT
                     GPRGVGKTSTARLLARCLNCEKGPTVDPCGECASCREILARGESLDVHEMDGASNRKI
                     ENARELIETIRYVPQRDRYRIAIIDEVHMLTSEAFNALLKTLEEPPPHSRFILASTEL
                     HKIPATIVSRCQRFTFRALSVAEIVRHLDRVAREEKIRATPEALETLAAAAEGSVRDA
                     LSLLDQAATLSGNNIDQAVCAEILALVDRELLESVYSAIATGDRGAAIASLSKLLEGG
                     EDPRHVLKEFIGLLRRLLLLSAGANPDVAEGDRDRMLALARAMPYDNLLRALALAVDA
                     DQLDRRTEEPGLVLQMLVLRLAELPRLSSIEEALSGLPAEGARPPAAPEPSTVRDGGS
                     RSREEGPRILSLVSVDAEPNELTSPPSMAPGSDERAVERFRQLLSARRRVTAAKVSLA
                     ESTTVEGDELVLRFGIDKAPAKEALDEPPTRKILSETASEAFGRPMRVRLQTGPPADG
                     DLGKAAQEVPRATIARERASSRAETDPVVRSAMELFRAELTEVKEEE"
     gene            complement(3867..3966)
                     /gene="ffs"
                     /locus_tag="VF958_06910"
     ncRNA           complement(3867..3966)
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="VF958_06910"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            complement(3994..4248)
                     /locus_tag="VF958_06915"
     CDS             complement(3994..4248)
                     /locus_tag="VF958_06915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9148877.1"
                     /translation="MFERPGPFPPARTVALGLLWLAIFSLLYTLGDESGAWRTIPVGR
                     FRDLELFVGFAGGVLALVALLRRPRQSRVPGPQPSIDRIG"
     gene            complement(4248..4502)
                     /locus_tag="VF958_06920"
     CDS             complement(4248..4502)
                     /locus_tag="VF958_06920"
                     /inference="COORDINATES: protein motif:HMM:NF024881.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-HC2 domain-containing protein"
                     /protein_id="HEX9148878.1"
                     /translation="MTDWTCKKVILDLLVEYEDGAMDAVDRTDFERHIEMCPPCVRFL
                     TTYRATGRTLKLLKPEEIPPDLAQSVLAFVRARTRREGDG"
     gene            complement(4499..5149)
                     /locus_tag="VF958_06925"
     CDS             complement(4499..5149)
                     /locus_tag="VF958_06925"
                     /inference="COORDINATES: protein motif:HMM:NF019265.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HEX9148879.1"
                     /translation="MAGEQPVVVASGDGAEPTDLDLVRRIKSGDEDAFEQMVERYHAR
                     VYSLSYGVLRNAEDAEEATQDTFLTLHRKIGTFDESKKFFSWFYRVALNQAYSRARRR
                     RPATTTLVEEFLPKFSADGHIASPDLPDWSASIEDGAIARDLANRAGEFMNELPPAYR
                     DVIWMYDVEEMSAADIAETLEISIPAFKSRLHRARLYVRQRLGEISRAPAREEVPR"
     gene            5226..5314
                     /locus_tag="VF958_06930"
     tRNA            5226..5314
                     /locus_tag="VF958_06930"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5260..5262,aa:Ser,seq:gga)
     gene            5492..>6647
                     /locus_tag="VF958_06935"
     CDS             5492..>6647
                     /locus_tag="VF958_06935"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HEX9148880.1"
                     /translation="MLGKAVSHYRVLEALGGGGMGVVYKARDEKLGRLVALKFLPEEM
                     AKDRQAMERMRREARSASSLNHPHICTIYDFDEYDGQPFIAMELLEGETLQQMLAKGP
                     LPTDRLLELGIQIADALDAAHSKGILHRDIKPANIFVTERGHAKVLDFGLAKPVPSRR
                     ALRHTDVSALATDIAEEHLTSPGVAVGTVAYMAPEQARGQDLDPRADLFSFGAVLYEM
                     ATGRPAFAGNTAAVIHDAILNRDPSPASRWNPNLPPDLERVIVKALEKDRELRYQTAA
                     ELRTDLKRIKRGSESAARISTVAPPPPRKRRIAPIAIGAALLVIGLAAGALWLVRRGR
                     PVAPQRSRWERLTHFTDSVTSPALSPDGRMLAFIRGPDTFTGPGQIYVKLL"
BASE COUNT         1117 a         2188 c         2230 g         1112 t
ORIGIN      
        1 ctgtcgcaag cgtgggctgg atgacccggg gaacgggacg ccggcgcagg tccatcagca
       61 cctcccgcgc ggcgatcttg agcgtctcga gaacgccttc tccgctgggc gcggtcgcct
      121 cgatcgtcgg ttcccccttg atgttcagcc gccgccagag ctcctcggcc gggagcgcgg
      181 agggaagatc tcgcttgttc aactggagca cgtaggggat ctccgcgagg tcccggtcat
      241 agctcttcag gtgcgcgtcc aggttgcgga tcgactcgac gttcgcgtgc agccgcgcct
      301 cctgcgagtc cgcgacgaag atgattccgt ctgcgccccg gagtacggcg cggcgcgcgg
      361 cgtcgtagaa gacctgcccc ggcacggtgt agaggttgaa acgcggccgg aagccccgca
      421 acggggcgag gtcgaccggg aggaagtcga agaagagcgt ccggtcggta tcggtggaga
      481 gagagatcag gcgtccgcgg agctccgggc gggcgtgctc ggaaaggtag cggaggttcg
      541 ccgtctttcc accgaggccg gggccgtagt agacgacctt gcagtggatc tcgcgggagg
      601 cgaagtcaat gaatgccatg tggggtcagg tcttcacgag tctcggcggc cggtcaacga
      661 gccgcaatgg tcttgccacg ttgcggagca atgtgacaag tggagacctg acccccatca
      721 ctccgagaac aggcggtcga tgtcctcgtc ggtgatctcg gccagcaacg gctcgaaggc
      781 ggaatgcaga accggcgcgc gctgcatgcg ttcgttgatc tcggcgaacg cctgcgccag
      841 ctcctgggcc gcctgtcgga cccggaggcg cacgagcccg agagaggaac ggtccgcgaa
      901 gatcaccacg agaatcacct tgccgccgac aacggagagg tggatgttct gcttctcccc
      961 ttcgtggagg aggacggaga actcgcgctc gccgagaatc cgggcgagtc cgtcggtcgc
     1021 cgcgacgttt ccggcggtca gcgacgcgag tgccgtgacg tccagggcat cgacgtctcc
     1081 ggccgaggcg atgcgctgcc cgttcttgtc cacgagaagg acgatcatcg cgtcggcctc
     1141 ctgccggagg cggcgcagta tctgctggag ccgccggaag tcttcgtcgt agagaacgag
     1201 atcggcggga gtcatcgact cattgtagcg aagaagcgtc gggacccggg acgctagagc
     1261 tctctgcggt tctccagaga ccgcgccatc gtcacttcgt cggcgtattc gatgtcgccg
     1321 ccgaccggaa gcccgaacgc gatccgggtg acgcggactc ccgacgtctt cagacgcttc
     1381 gcgatgtaga gggccgtcgt ctccccttcg acgttcgggt tcgtcgccag aacgacctcc
     1441 cggatctcgg gcgtcacgcg ggcgacgagg ccgccgatgt cgatctcgtc cggaccgatt
     1501 ccccggagcg gcgaaagcgc cccccagagg acgtggtagc ggccgcgaaa ctcccgggtc
     1561 ttttcgatcg cggcgacgtt gccgggctcc tcgacgacgc agaggagcga cgcgtcgcgc
     1621 tgcgggtccg agcagatctc gcaggggtcg tcctcggtca gggtgttgca gacggagcag
     1681 cgccggacgg cgtcccgcga ctcggtgatc gcggaagcga gtcgttccgc ctcatcgcgc
     1741 ggcgatcgga gcagatagta ggcgagtcgc gaggcgctct tggcgccgat tccggggagc
     1801 cgctggaatt cagagacgag ccggtcgagc cccttgagtt tcaacctacc gcattcccgg
     1861 gagcatgccg ccgagcatcg aggaggtctt cttctgaacg gcttcgtcga cgcgccgggt
     1921 ggcatcgcgc agggcggcga gaacgaggtc ttcgagcgtc tcgacgtcct cccggttcac
     1981 cgcggaagga tcgatcttca ccgccaggac ttccttcgcg ccgctcatct cgatcgagac
     2041 gagccctccc cccgcggaac cctgcaggcg catggcctcg agctccgcct gcaccttgct
     2101 ctgcatcgcc tgggcctgct gcatcatttt ctggatgttc atgggtgcgg cgcgccgcta
     2161 ctcctcctct ttgacttccg tgagctccgc gcggaagagc tccatcgcgg agcggacgac
     2221 cgggtccgtc tccgcccggc tcgacgcgcg ctcccgggcg attgtcgcgc gcgggacttc
     2281 ctgagccgct ttcccgaggt cgccgtcggc gggcggtccg gtctggagcc ggacgcgcat
     2341 cggccgaccg aaggcttcgg acgccgtctc ggacaggatc ttgcgggtcg ggggctcgtc
     2401 gagcgcctct ttcgccggcg ctttgtcgat gccgaagcgc agcacgagct cgtcgccctc
     2461 cacggtcgtc gactccgcga ggctcacttt cgcagccgtg acgcggcgtc gcgcggagag
     2521 caactgccgg aatcgctcca ccgccctctc gtcgctcccc ggcgccatcg atggcgggga
     2581 cgtcagttcg ttcggctcgg cgtccaccga caccagggag aggatgcggg gaccctcctc
     2641 gcgggatcgg gaaccgccgt cgcgcaccgt cgaaggctcg ggcgccgcgg gaggacgggc
     2701 gccctccgct ggcaggcctg agagcgcctc ctcgatcgag ctcaatcgcg gaagctccgc
     2761 gagccgcaga acgagcatct ggaggacgag gccgggctcc tccgttcgcc ggtcgagctg
     2821 gtccgcatcg accgcgagcg caagcgcgcg gagaagattg tcgtaaggca tcgcgcgggc
     2881 gagcgcgagc atccggtcgc ggtcgccttc ggcgacgtcc gggttcgcgc ctgcggaaag
     2941 aaggagtagc cgccgcagaa ggccgatgaa ctccttgaga acgtgccgcg ggtcctcccc
     3001 tccctcgagg agcttcgaga ggctggcgat ggcggcgccc cggtccccgg tcgcgatggc
     3061 cgagtagacg gactcgagca gttcgcggtc cacgagagcg agaatttccg cgcagacggc
     3121 ctggtcgatg ttgttccccg agagcgtcgc cgcctgatcg agcaacgaga gcgcgtctct
     3181 cacgctccct tcggcggcgg cggccagggt ctcgagggct tccggcgtcg cccggatctt
     3241 ctcttcgcgg gcgacgcgat cgaggtggcg cacgatttcg gcgacggaaa gcgcgcggaa
     3301 cgtgaaccgc tggcagcgcg agacgatcgt cgccggaatc ttgtgcagtt cggtggacgc
     3361 gagaatgaac cgcgagtgcg gcggcggctc ttcgagcgtc ttcaggagcg cgttgaacgc
     3421 ctcgctcgtc agcatgtgga cttcatcgat gatcgcgatc cggtagcggt cgcgctgcgg
     3481 gacgtagcgg atcgtctcga tgagctctcg cgcgttctcg atcttccggt tcgacgcgcc
     3541 gtccatctcg tgaacgtcca gcgactcccc gcgcgcgagg atctcgcggc aggaggcgca
     3601 ctccccgcag ggatccacgg tgggaccctt ttcgcagttg aggcatcggg cgagaagcct
     3661 cgccgtcgac gtctttccga cgccacgtgg accggtcagg agatacgccg gcgcgatacg
     3721 cacctcggag attgcgttca tcagcgtgcg cgcgaccgag tcctgaccga tcaggtctgc
     3781 gaacgtccga ggacgccact tgcggggaag ggagagatgt gccacgttca agatcggatc
     3841 ttagacggag ttccggcggc cgaagatgga ttcaggttga cctgcggcgc acggggacgc
     3901 ctcctgatcg ctgcttcctt ccggacctga cgaggttcgg agacttccgt cgcacagggc
     3961 ccgaacccat cctcggccgc cggaatgccg attctatccg atccgatcga ttgagggctg
     4021 cggtccgggg acccgggact gacgaggtct ccgaaggaga gcaacgagag ccagaacacc
     4081 gcccgcaaac ccgacgaaga gctcgagatc tcgaaatctt ccgaccggaa tcgtgcgcca
     4141 ggctcccgat tcgtcgccga gcgtgtacag cagcgagaag atcgccagcc acaggagacc
     4201 gagcgcgacg gttcgggccg gtgggaaggg gcccggacgc tcaaacatca gccgtcgccc
     4261 tcgcgccgcg tccgagcgcg cacgaacgcg agaaccgact gcgcgagatc cggaggtatt
     4321 tcctcgggct tcagaagctt cagggttctc ccggtggcgc ggtaggtcgt caggaagcgg
     4381 acgcagggcg ggcacatctc gatgtgccgc tcgaagtcgg tccggtcgac cgcgtccatc
     4441 gccccgtcct cgtactcgac caggagatcc aggatgactt tcttgcaggt ccagtcggtc
     4501 atcggggcac ctcttcacgg gcgggcgcgc gcgagatctc acccagccgc tgccgcacgt
     4561 agaggcgcgc gcggtgcagc cgcgacttga acgcggggat cgagatctcc agggtctccg
     4621 cgatgtccgc cgcgctcatc tcttcgacgt cgtacatcca gatgacgtcg cgataggccg
     4681 gcgggagctc gttcatgaac tcgccggcgc ggttggcgag gtcgcgcgcg atcgcgccgt
     4741 cttcgatcga agcgctccag tccgggagat cgggagaggc gatgtgcccg tcggccgaga
     4801 acttcggcag gaactcctcg acgagggtcg tcgtcgccgg ccggcggcgg cgagcgcggc
     4861 tataggcctg gttcagagcg acgcggtaaa accaggagaa gaacttcttc gactcgtcga
     4921 aggtgccgat cttccggtgc agcgtgagaa acgtgtcctg agtcgcctcc tcggcgtcct
     4981 ccgcgttgcg caggactccg taggagagcg aatagacccg ggcgtgatag cgctcgacca
     5041 tttgttcgaa ggcgtcctcg tcccccgact tgatgcggcg caccaggtcg agatcggtcg
     5101 gctcggcgcc gtcgcccgat gcgacgacga ccggttgctc accggccatg gaaaagacgc
     5161 ttcggaacgc ggaaaggatt caaggaatga ggattctacg acgctgcgtt acgatgcggt
     5221 ttcccggaga ggtgtccgag tggcttaagg agcacgcttg gaaagcgtgt gtgcccaaaa
     5281 ggtaccgagg gttcgaatcc ctccctctcc gccacgaact ccttgcgtcg cagccggtca
     5341 atgaacgaca gccctgactg ctctatctca acgggccagt ctaaccgggc gcgagggttc
     5401 gcaccgagcg cccgaagggc gcgacaagcg acggcgtcga gccgtcgcta atccctccct
     5461 ctccgccaca taatctctct cgcaccctcc tatgctcgga aaagccgtct cccactaccg
     5521 cgtcctcgag gccctcggcg gcggcgggat gggcgtcgtt tacaaggccc gcgacgagaa
     5581 gctcggacgc ctggtcgcct tgaagttcct gcccgaagaa atggcaaagg atcggcaggc
     5641 aatggagcgc atgcgtcgag aggcgcgttc ggcctcctct ctcaaccacc cgcacatttg
     5701 cacgatctac gacttcgacg aatacgacgg gcagccgttc atcgccatgg agctgctcga
     5761 aggcgagacg cttcagcaga tgctcgccaa agggccgttg ccgaccgacc ggctgctcga
     5821 actcggtatc caaatagccg acgcgctcga cgcggctcac tccaagggaa tcctccatcg
     5881 cgacatcaag ccggcgaaca tcttcgtgac cgaacgcggg cacgccaagg tcctggactt
     5941 cgggctggcg aagccggtgc cgagccggcg cgcgcttcgg catacggacg tctctgccct
     6001 tgccaccgat atcgccgaag aacacctgac gagtcccgga gtcgcggttg gaaccgtcgc
     6061 ctacatggcg ccggagcagg cgcgaggcca ggatctcgat ccccgggccg acctgttttc
     6121 cttcggcgcg gttctctatg agatggcgac aggaaggccc gcattcgccg gcaacacggc
     6181 cgcggtgatc cacgacgcga ttctcaatcg agatccttct ccagcgagcc gctggaatcc
     6241 gaatctgccg ccggatctcg aacgggtcat cgtcaaagcg ttggagaaag accgcgagtt
     6301 gcggtaccag acagccgccg agctgcgcac ggacttgaag cgcatcaaac gcggatcgga
     6361 gtccgcggca aggatctcca ccgtcgctcc gccaccgcca cgcaaacgac gcatcgctcc
     6421 gatcgcaatc ggcgccgcgc tcctggtcat cggccttgca gccggtgcgc tctggctcgt
     6481 tcggcgcggt cgtccggttg caccccaacg ctcccgttgg gagcggctca cccatttcac
     6541 cgattccgtc acgtcgccgg cgctttcccc cgacgggcga atgctcgcct tcattcgagg
     6601 cccggacacc ttcaccgggc ccgggcagat ctacgtcaag ctgctgc
//
LOCUS       DASWAV010000337         1938 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U2665
            k141_738383, whole genome shotgun sequence.
ACCESSION   DASWAV010000337 DASWAV010000000
VERSION     DASWAV010000337.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960316
            Sequence Read Archive: SRR10260007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 1938)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1938)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:47:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,929
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1938
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_738383"
                     /isolate="SMAG_U2665"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.36444 N 132.31889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..824)
                     /locus_tag="VF958_06940"
     CDS             complement(<1..824)
                     /locus_tag="VF958_06940"
                     /EC_number="1.1.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007907161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent isocitrate dehydrogenase"
                     /protein_id="HEX9148881.1"
                     /translation="MSENVPITVAAGDGIGPEIMAATLHILKAAGARVAIEEIEIGEK
                     VFLRGLSSGIEPSAWESLRRTRVFLKAPITTPQGGGFKSLNVTARKTLGLYANIRPCV
                     AYHPFVRTHHPGMDVVIVRENEEDTYAGIEYRQTEDVVQCLKLISRPGSEKIVRYAFE
                     YARRNQRRKVTCFTKDNIMKFTDGLFHRVFEEISAEYPDIEKEHWIVDIGAAKLADSP
                     ETFDVIVLPNLYGDILSDVAAQIAGSVGLAGSANIGERCAMFEAIHGSAPRRAGQNL"
     gene            926..1282
                     /locus_tag="VF958_06945"
     CDS             926..1282
                     /locus_tag="VF958_06945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9148882.1"
                     /translation="MAEPIRFRETTEIASSRVDARDGLALRFDPTRRLTLRFRLQLES
                     DEDVEALRYARRIMIREERTRGLEWEEPSVEDAVFTLTDVSWSALASQAAWCREKFRE
                     LVERANRVRGEIAGID"
     gene            1468..1764
                     /locus_tag="VF958_06950"
     CDS             1468..1764
                     /locus_tag="VF958_06950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9148883.1"
                     /translation="MLPTSVAEVQAGSARGLTLRFLVSADGGRSLDALRDARRSLLRE
                     ELTRGREPDEPSLEDAVFSTHEILWEVRPEQRLWCLQKLEELRRRANLLLTEVR"
BASE COUNT          306 a          630 c          635 g          367 t
ORIGIN      
        1 aggttctggc cggcacgtcg cggcgcggag ccatggatcg cctcgaacat cgcgcagcgc
       61 tccccgatgt tggcggaccc ggcgagcccg accgagccgg cgatctgcgc cgcgacgtcc
      121 gagaggatgt cgccatagag gttcggaaga acgattacat cgaacgtctc gggcgagtcc
      181 gccagcttcg ccgcgccgat gtcgacgatc cagtgctctt tctcgatgtc cgggtactcg
      241 gcggagatct cttcgaagac gcggtggaag agaccgtcgg tgaacttcat gatgttgtct
      301 ttggtgaagc atgtgacctt gcgtcgctgg ttgcggcgcg cgtactcgaa cgcgtagcgc
      361 acgatttttt cggagcctgg acggctgatc agcttcaggc actggacgac gtcttccgtc
      421 tgccggtact cgatcccggc ataggtgtct tcctcgttct cgcggacgat cacgacgtcc
      481 atgccgggat ggtgggtgcg gacgaagggg tggtaggcga cgcacggccg gatgttggcg
      541 tagaggccga gggtcttgcg cgcggtgacg ttcaggctct tgaagcctcc gccctgcggc
      601 gtcgtgatcg gggctttcag gaagacccgg gtccgccgga gggactccca cgccgacggc
      661 tcgatcccgg aactcaagcc gcgcaggaag accttctccc cgatctcgat ctcctcgatg
      721 gccacccggg cccctgcggc cttcagtatg tgaagggtcg cggccatgat ttccgggccg
      781 atcccgtcgc cggcagcgac ggtaataggg acgttttcag acatgtgtaa cctccgaaaa
      841 gcgctgcatt atgctccgat caagcgggtg acaagtggga cggttgcatc aggcggcgga
      901 cgtataattt ccctccagat cccacatggc ggagccgatc cgtttccggg agaccaccga
      961 gatcgcgtcg tcccgagtgg acgcccgtga cggcctcgct ctgcgattcg acccgactcg
     1021 ccggctcacc ctgcgattcc ggctccaact cgagtccgat gaagacgtgg aagcgctccg
     1081 ttacgcccgg cggatcatga tccgcgagga gcgcacacga gggctcgagt gggaggaacc
     1141 atccgtggag gatgccgtct tcacgctgac ggacgtctcc tggtcggcac tcgcttccca
     1201 ggcggcgtgg tgccgcgaga agtttcgcga gctcgtcgag cgcgccaacc gggttcgggg
     1261 ggaaattgcc ggtatcgatt gatccgaccc cgtcacgctg tcaccttcgg taacactttc
     1321 ccacaggcga tcgcggccgc ccccccgcgg tctttctcca agtcgttcgc ttccggcgaa
     1381 ttgcgggcgc cgcacccggc ggcacacgct tcgctacttc tctcccccgg cgtgtccgag
     1441 atcggtttgg tcgaaatctc gggagggatg ctgccgactt cggtcgccga agtacaggcg
     1501 ggttctgccc gcgggctcac gcttcggttc ctggtttccg ccgacggcgg acgcagcctg
     1561 gacgctctgc gggacgcacg ccggtcactc cttcgcgagg agttgacgcg gggtcgcgag
     1621 cccgacgagc cgtcgctcga ggacgcggtc ttctcgaccc acgagatcct ctgggaggtt
     1681 cgaccggaac agcgcctctg gtgccttcag aagctcgagg agcttcgccg ccgcgccaac
     1741 ctgctgctga ccgaagttcg ctgaccgcgg gatcgaaccc cgggcttaca attctcgaga
     1801 atgggcggac gacgggtccg cggcctgctc acaatcgccg cactcctcgc ggtcggatgg
     1861 tggatctacg cgaaacggcc caccttttcg ggagtcgtcg acgatctgac ccggccgctc
     1921 ttcgggtccc gcgcggcc
//
LOCUS       DASWAV010000338         3507 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U2665
            k141_575710, whole genome shotgun sequence.
ACCESSION   DASWAV010000338 DASWAV010000000
VERSION     DASWAV010000338.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960316
            Sequence Read Archive: SRR10260007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 3507)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3507)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:47:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,929
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3507
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_575710"
                     /isolate="SMAG_U2665"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.36444 N 132.31889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..390)
                     /locus_tag="VF958_06955"
     CDS             complement(<1..390)
                     /locus_tag="VF958_06955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011735553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HEX9148884.1"
                     /translation="MKTFYATTPIYYVNDLPHIGHIYSTVVGDVLTRYHRLIGEKTRY
                     LTGTDEHGQNIERAAAAQGIAPIELADRVVARYHELWRTFGIANDDFIRTTEPRHRRG
                     VEELVRRIEASGDLYLDRHEGWYCTSCE"
     gene            complement(507..1199)
                     /gene="radC"
                     /locus_tag="VF958_06960"
     CDS             complement(507..1199)
                     /gene="radC"
                     /locus_tag="VF958_06960"
                     /inference="COORDINATES: protein motif:HMM:TIGR00608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HEX9148885.1"
                     /translation="MTPETFRIRDLPLEERPRERLARAGPGALSGEELLALLLGSGAR
                     GGSALDCARRLLAAHGGLAGLASLTGSELARERGLGRARASAIEAAIEIGRRLAAETL
                     SARDLFNEPALVKEYLRRTHGDGTQERTGALYLNARNRLLRDDGEIYRGTLDRAVVEP
                     RELLKRALLANAAALIVYHNHPSGDPSPSREDREFTRRLASAADLVGLRLLDHVVVGR
                     EGCVSFREAGLL"
     gene            1215..1427
                     /locus_tag="VF958_06965"
     CDS             1215..1427
                     /locus_tag="VF958_06965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019120119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2905 domain-containing protein"
                     /protein_id="HEX9148886.1"
                     /translation="MGGSPVRALLLVFGILFVVAALAWPVVSRYFGRLPGDIVVRRPG
                     FTFVFPIVTCLVLSLLLSLLFWIFRR"
     gene            complement(1369..2538)
                     /locus_tag="VF958_06970"
     CDS             complement(1369..2538)
                     /locus_tag="VF958_06970"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEX9148887.1"
                     /translation="MSVPPLSILQVLEKGSFTTGSVVQMFELARGLARRGHRVGVVSR
                     PDGEVGERSGEEGIPFYPLPLSHEFNLASARGLALLFEERDVDVVHVHKGIAHSAALF
                     ATLFARRRPVLVVNRGVSFPLDFFAGLKFRVRLDAVVAVSEDIKRIVVATGGVPPEKV
                     HVVYAGVDLSRFDPARADGARVRREWNVADDEKLVLQVGARDWKGWTDLVSAAALLAR
                     DFRRLRTAIVACKDEAEKERVRAFAREKGIAERVIPIGFRTDMPDVLAAADVVADLSY
                     EGLGITGTIREAMALGRPVVASAAGGNPELVEHEVSGLLVPPRDPVATAAALSRVLSS
                     PDLAGRLGRAARARVAEGFSTEVRLDRIEALYRTLVATSGRSRRGARGVATIRDR"
     gene            complement(2572..>3507)
                     /locus_tag="VF958_06975"
     CDS             complement(2572..>3507)
                     /locus_tag="VF958_06975"
                     /inference="COORDINATES: protein motif:HMM:NF024989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HEX9148888.1"
                     /translation="HLDIFVRNGIPDVPLPQVPGADGSGVVQAIGEGVSDLAIGDRVL
                     VQPGLYCGACEFCRSGEQTLCVRYGIVGEHAPGTFAEKALLPARNLFPIPDGLSFEQA
                     AAFPLSYETAWRLIVTRGSVRPGDTVLIHGVGGGVAWAALEIALLCGARVIATTSGEQ
                     KARAALAAGAELVIDYQKEKVADAVRRHTARRGVDVVVDSVGEKTWMTSLKSVRRGGR
                     IVTCGATTGPNPVEEIRLIFWKQFAILGSTMANDREFRALLQTVAAGRLTARIDRVFP
                     LSEARAAYELLESGRQFGKVVLVPEAGGREWPDGD"
BASE COUNT          548 a         1242 c         1185 g          532 t
ORIGIN      
        1 ctcgcagctc gtgcagtacc acccctcgtg gcggtcgagg taaaggtcgc cggacgcttc
       61 gattcggcgc acgagttcct cgacgccgcg gcggtgccgc ggttccgtcg tccggatgaa
      121 gtcgtcgttg gcgatcccga aggttcgcca cagctcgtgg taccgcgcga cgacccggtc
      181 cgcgagctcg atgggtgcga tcccctgcgc ggccgcggcg cgctcgatgt tctggccgtg
      241 ctcgtcggtc cccgtcagat accgcgtctt ctcgccgatc agccggtggt agcgggtcag
      301 gacgtcgccc accaccgtcg agtagatgtg cccgatgtgg ggcagatcgt tgacgtagta
      361 gatcggcgtc gtcgcgtaaa aggtcttcat gggagtcggg aaagataaca ggaaggggtc
      421 aggtcttcac tgatcaccac agcggctgac gtgccgcggc agccttgcca ccttgcgcgg
      481 caagtggcaa gtggagacct gaccccctac aaaagacccg cctcgcggaa agaaacgcag
      541 ccctcccgcc ccacgacgac gtgatcgagg agccgcaacc ccaccaggtc cgccgcggag
      601 gccaggcgcc gcgtgaattc gcgatcttcg cgcgacggcg acggatcgcc cgaaggatgg
      661 ttgtggtaca cgatcaacgc cgccgcgttc gcgaggagcg cgcgtttcag gagctcccgc
      721 ggttcgacga cggcgcggtc gagcgtgccg cggtagatct cgccgtcgtc ccgcaggagc
      781 cggttgcggg cattcaggta cagagcgccc gtgcgttcct gggtgccgtc gccgtgcgtg
      841 cggcggaggt attccttgac gagcgccggt tcgttgaaga ggtctcgcgc ggagagcgtc
      901 tcggccgcga ggcgccgtcc gatctcgatc gcggcctcga ttgcggaggc gcgcgctctc
      961 cccaggcctc tctcgcgcgc gagctcgcta cccgtcaggc tggcgaggcc ggccaggccg
     1021 ccgtgcgctg cgagcaggcg gcgcgcgcag tcgagcgcgc tccccccgcg ggcgccggac
     1081 cccaacagga gcgcgaggag ttcctcgccg gagagcgcgc ccgggccggc ccgcgcgaga
     1141 cgttcgcggg gacgctcttc gagcggcagg tcgcggatcc ggaacgtctc gggcgtcatg
     1201 ggatgattcg ctcgatggga ggctcgccgg tccgcgcgtt gctcctcgtc ttcggaatcc
     1261 tcttcgtcgt ggcggcgctc gcgtggcccg tggtgtcccg atacttcggc cgcctccccg
     1321 gcgatatcgt cgtccgccgc cccgggttca cgttcgtctt cccgatcgtc acctgtctcg
     1381 tattgtcgct actcctctcg ctcctcttct ggatcttccg gaggtagcga caagcgtccg
     1441 gtacagcgct tcgattcggt cgagccgaac ctccgtcgag aacccctccg ccacgcgcgc
     1501 gcgcgcggcg cgacccagcc ggccggcgag atccgggctc gagagaacgc gggagagcgc
     1561 cgccgccgtc gcgaccgggt cgcgcggggg cacgaggagt cccgagacct cgtgctccac
     1621 gagctccggg ttgcccccag cggcgctcgc cacgacgggc ctgccgagcg ccatcgcctc
     1681 gcggatcgtg ccggtgatgc cgagtccctc gtaggagaga tcggcgacga cgtcggcggc
     1741 cgcgaggaca tcgggcatgt cggtccggaa cccgatcggg atcactctct ccgcgatccc
     1801 cttctcgcgg gcgaacgcgc gaacgcgctc cttctccgcc tcgtccttgc aggcgacgat
     1861 cgcggtgcgg agccggcgaa aatcccgcgc gagaagcgct gcggcggaca cgaggtcggt
     1921 ccaccctttc cagtctcgcg ctccgacctg aaggacgagc ttctcgtcgt cggcgacgtt
     1981 ccactcccgc cgcacccgcg ctccgtcggc gcgggcggga tcgaagcggg agaggtccac
     2041 ccccgcgtag acgacgtgca ccttctccgg cggaacgcct cccgtcgcga cgacgatcct
     2101 cttgatgtct tcggacacag cgacaaccgc gtcgagtcgc acccggaact tcaagccggc
     2161 gaagaagtcg agggggaacg agacaccgcg gttgacgacg agcaccgggc ggcggcgggc
     2221 gaacaacgtc gcgaagaggg cggccgagtg cgcgattccc ttgtggacgt ggacgacgtc
     2281 gacatcccgt tcctcgaaga gaagcgcgag gccccgggcg ctcgcgaggt tgaactcgtg
     2341 ggagagcggg agcggataga acggaatccc ctcctcgcca ctgcgctctc cgacttcccc
     2401 atccggccgt gagacgacgc ccacgcggtg tccgcgacga gcgagtccgc gcgcgagctc
     2461 gaacatctgg acgacgctgc cggtggtgaa gcttcccttc tcgagcacct ggagaataga
     2521 aagcggcggg acgctcaacc cggaacctcg aacctcgacc ttcgaacccc gctagtcacc
     2581 gtcgggccac tctcgccccc ccgcttcggg aacgagaacg acctttccga actgccgccc
     2641 cgattccagg agctcgtacg cggcacgagc ttcggagagg ggaaagacgc ggtcgatccg
     2701 agccgtcagc cgcccggcgg cgacggtctg gaggagcgcc cggaactcgc ggtcgttcgc
     2761 catcgtcgag cccaggatcg cgaactgctt ccagaagatc agccggatct cctccaccgg
     2821 gttcgggccg gtcgtcgcgc cgcaggtcac gatccgaccg ccgcgccgga cgctcttcaa
     2881 cgacgtcatc caggtctttt ctccgacgga gtcgacgacg acgtccacgc cgcgccgggc
     2941 ggtgtgccgt cgaacggcat ccgcgacctt ttccttctgg tagtcgatca cgagctccgc
     3001 gccggccgcg agcgccgccc gggctttctg ctcccccgac gtcgtcgcga tgacccgggc
     3061 gccgcaaagg agagcgatct caagcgccgc ccacgcgacg ccgccgccca caccgtggat
     3121 gagcaccgtg tcgccgggac gcaccgaccc gcgggtcacg atcaggcgcc acgccgtctc
     3181 gtacgagagc ggaaacgcgg ccgcctgctc gaacgagagt ccgtcgggaa tcgggaagag
     3241 atttcgcgcc ggaaggagag ccttctcggc gaaggtgccc ggcgcgtgct cgccgacgat
     3301 cccgtacctg acgcagaggg tctgctctcc cgaacggcaa aactcgcacg ccccgcagta
     3361 gagcccgggc tggacgagaa cgcgatcacc gatggcgaga tccgaaacgc cttccccgat
     3421 cgcttggacg acgcccgagc cgtcggcgcc gggcacctgc ggaagcggaa cgtccggaat
     3481 cccgttgcgc acgaagatgt ccaggtg
//
LOCUS       DASWAV010000339         7005 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U2665
            k141_419047, whole genome shotgun sequence.
ACCESSION   DASWAV010000339 DASWAV010000000
VERSION     DASWAV010000339.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960316
            Sequence Read Archive: SRR10260007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 7005)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7005)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:47:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,929
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7005
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_419047"
                     /isolate="SMAG_U2665"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.36444 N 132.31889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1152
                     /locus_tag="VF958_06980"
     CDS             <1..1152
                     /locus_tag="VF958_06980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011956288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9148889.1"
                     /translation="KSTDGGRTWRTIVTGPFGTGPTNLAIDPFDPSRLYAAGCPAFSR
                     STDAGETWTQAAAPPSNNCASTLVADPVRPGYLYTGFWGGGVWRSRDGGNTWQPFGEG
                     ISGFAVRLAIDPAGLFLHASTSRGVFDIRLAETRATSCDPGPERLCLLGGRFEVTVKI
                     EDSAAQAVPQGDRFGSFRRPGAGPADPEIFVKMADHRTASNRAFWFFHSSVTDLPYTI
                     SVTDTATGRIRFYRNEPVNPGCGAADTGAFRETSASPSSDEARVESNLAPGEGEILPL
                     YNGRYEVTLSVVNPFTGTIEAGLAMPQSDRLGYFSFPSLTGDPAFPEVFVSLSVLSFY
                     EFKFSHTGLTNLQYTLTLTEVGSDISTTYRNNGADVFNLCGGSSSWSDL"
     gene            1222..1566
                     /locus_tag="VF958_06985"
     CDS             1222..1566
                     /locus_tag="VF958_06985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9148890.1"
                     /translation="MAMIPEVFECPRCERQFVIDGVPDTGSEEVHCPQCSRFLGVRDI
                     AFVLRTELDELIERLSFEKDRLEKDEAIYGLTDCDPEMKYYGREVSRVIGVLRGALKD
                     LHRLREDYRTKN"
     gene            complement(1520..3019)
                     /gene="gltX"
                     /locus_tag="VF958_06990"
     CDS             complement(1520..3019)
                     /gene="gltX"
                     /locus_tag="VF958_06990"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015738553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009332 - glutamate-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HEX9148891.1"
                     /translation="MRTTFRKLACGVLANTDGPRVRFAPSPTGFLHVGGARTALYNLL
                     FARRERGTYILRIEDTDVERSREELSGQILSALDWLGLQHDEGPYYQSKRSDLYRAAA
                     DRLIAEGKAFRAFETPEELDAARKKAESEGRSYRYSGAGRDLAPDESDRRARAGEKFV
                     VRLKMPHEPIVVDDLIRGRVEFPADALDDFVLVRSDGHPLYHFTVCVDDADMRITHVL
                     RGDDHLANTPKHVALFRALGAEVPAFAHLGMILGTDRKKLSKRYGAVAVEEWREQGIF
                     PEALVNFLALLGWSPGDDREILTREEMEREFSLERIGASPSVFDPEKLLWVNAQYMAR
                     MPAEELLKRAEPYAPAGMPEREAALAAVELHRTRARTAIEMGRALSVYAKDPEEYDTE
                     GMRKNVRPETAAEIGRLIATYEKVEAWTARELDAALRQLAAEMKISASRLIHPVRIAL
                     TGVTVGAPLFDVVALLGKETALRRLRRFVEKIRAASSSSGSLPAGGGGP"
     gene            3037..3939
                     /locus_tag="VF958_06995"
     CDS             3037..3939
                     /locus_tag="VF958_06995"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEX9148892.1"
                     /translation="MERTEPVSMRLRADVALVLVTLVWGSSFVVVKNVLVEAPPLAFV
                     FFRFLIATVVCLPLTFRRPRSPDLLRDGAIIGSLLAAGMIFQVLGQTETSASKAAFLT
                     GLSVVLTPFAAFLRTRQLPSLENGVGIALAGMGFFLLTLPQGGEPINRGDVLVFACGV
                     VFAFYIVELGVRAGRHDASRLTVIQLAVVAAVAGMVAVVFRAAGAAVESRPIVWGGTF
                     LRSVLYLATIGTIGTFLTQTWAQRHMSSTHAAIIFALEPVWAALLAAWLLGERLETVG
                     YWGAALVLAGIVVSELRLRSRCKP"
     gene            complement(3992..5284)
                     /locus_tag="VF958_07000"
     CDS             complement(3992..5284)
                     /locus_tag="VF958_07000"
                     /inference="COORDINATES: protein motif:HMM:NF012318.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HEX9148893.1"
                     /translation="MKRTLAVLLAIAAHGAAAAAPLPSRRSEIVDVVEKVSPAVVNIS
                     AEQTVQRRPSLVDEFFFGVDPRPRRSQSLGSGVIIRPEGIILTNDHVISGASRIIATT
                     KSGKELECDVVGSDADNDLAVLRVRNSRGNLPTIRLGTSSDLLIGETIVAIGNPFGLS
                     NTVTAGVVSALGRSVPGENQRMYTDFVQIDAPINPGNSGGPVVNIQGDMIGVATAIIG
                     GAQGIGFAIPVDRARRIVDDLVRFGEVRAVWIGVRGQTLTSGNAELARPGGFRIRSVT
                     PASPAARAGVRSGDLIVSVDGTPIDSREAFETALSTRGPGRPMRLLLRGSGGERTITV
                     QGQAPPADLGSQLLREQLGITVSAARGGLRINVVDRNGDAADRGIESGDVLLGLNGTR
                     VTTVADVNRIVQRDYNRTTLLMEVGRGRFAYTLTFPLD"
     gene            complement(5386..5877)
                     /locus_tag="VF958_07005"
     CDS             complement(5386..5877)
                     /locus_tag="VF958_07005"
                     /inference="COORDINATES: protein motif:HMM:NF012241.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HEX9148894.1"
                     /translation="MALIRWDPYRELSSFADRFNRTFGSVPAREREEEVGLGAWMPPV
                     DIAEDKEKITLTAELPGFKENEVQIQMEGNLLTLRGDRKFEEEKNGRNYHRVERSYGQ
                     FIRSFTLPNNVDRENIRARFNSGLLEVEMPKREDAKPKQIKISGDTASGHAEKKPIDV
                     QTR"
     gene            complement(5916..>7005)
                     /locus_tag="VF958_07010"
     CDS             complement(5916..>7005)
                     /locus_tag="VF958_07010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010941319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEX9148895.1"
                     /translation="ERELAANNTRLADLQKGGRPMLREEVTEEDIAAVVSRWTGVPVS
                     RMLETETQKLLHMEDRLAQRIVGQREAVRAVSDAVRRSRAGLSDPRRPIGSFLFVGPT
                     GVGKTELARALAEFLFDDEQAMVRLDMSEYMEKHSVARMIGAPPGYVGYEEGGRLTEA
                     IRRRPYAVVLFDEIEKAHPDVFNILLQVLDDGRLTDGKGRVVSFKNTVLIMTSNLGSE
                     WIAEAQDMNEAEVKARIEETLRRHFRPEFFNRIDDVIVFHRLTREETQEIVEIQLQSL
                     ARTLADRGVRLTWSEEAKRYLAGRGYDPTFGARPLKRLIQKEVSDALAGKILRNELAP
                     GDTAQVTVSTDGESLEIETESEPRVLSV"
BASE COUNT         1080 a         2324 c         2361 g         1240 t
ORIGIN      
        1 aagagcacgg atggcgggag gacgtggagg acgattgtca cggggccgtt cggcaccgga
       61 ccgaccaacc tcgcgatcga tccgttcgat ccctcccgtc tctacgcggc cggttgtcca
      121 gccttctccc ggagcaccga cgccggtgag acctggactc aggcggcggc gcccccctcg
      181 aacaactgcg ccagtactct cgtggcggac ccggtgaggc cgggctacct ctatacgggc
      241 ttctggggcg ggggagtctg gcggagccgc gacggcggaa acacgtggca gccattcggc
      301 gaagggattt ccgggttcgc ggtgagactc gccatcgacc ccgccggact ctttctgcac
      361 gcgtcgacga gccgcggcgt cttcgacatc cggctcgctg agactcgagc gacctcgtgc
      421 gatccgggac ccgagcgact ctgtctcctc ggcggccgat tcgaagtgac cgtgaagatc
      481 gaagattccg cggcccaggc agtcccgcag ggcgaccgct tcgggtcctt ccgccgtccg
      541 ggagcgggtc ccgccgatcc ggagatcttc gtcaagatgg ccgaccaccg gaccgcttcg
      601 aaccgggcgt tctggttctt ccacagcagc gtcacggatc tcccctacac gatctcggtg
      661 acggacaccg ccaccgggcg catccggttt tatcgaaatg agccggtgaa tccgggctgc
      721 ggcgccgcgg ataccggcgc gtttcgggag acgtcggcct cgccgtcgag cgacgaggcg
      781 cgcgtcgaat ccaacctcgc tccgggcgag ggggagatcc tccctctgta taacggccga
      841 tacgaggtca cgctctccgt ggtgaatccg ttcacgggga cgatcgaagc cggcctggcc
      901 atgcctcagt cggatcgact cggttatttc agctttccgt ctctgaccgg cgatccggcg
      961 ttcccggagg tctttgtctc gctctccgtg ttgagtttct acgaattcaa gttctcgcat
     1021 acgggtctca ccaacctgca gtacacattg actctgaccg aggtcggctc cgacatcagc
     1081 acgacctatc ggaacaacgg cgccgacgtc ttcaacctct gcggcgggtc gagctcgtgg
     1141 tcggacctgt gagccggccg gggccctctc cccgcgcgcc ggggggaggg ccgatcatct
     1201 cgcagaggag ttacactcgg cgtggccatg attccggaag tcttcgagtg cccccgctgc
     1261 gagcggcagt tcgtgatcga cggcgtcccc gacacgggaa gcgaagaggt ccactgcccg
     1321 cagtgcagcc ggtttctcgg cgttcgcgac attgcgttcg ttttgcgcac ggagctcgac
     1381 gaattgatcg agcggttgtc cttcgagaag gaccggctgg aaaaagacga ggccatctac
     1441 ggattgaccg actgcgaccc ggagatgaag tactacgggc gcgaggtgtc gcgggtgatc
     1501 ggagtgctgc ggggagcgct caaggacctc caccgcctgc gggaagacta ccggacgaag
     1561 aactagccgc gcggatcttc tccacgaacc tccggagtcg ccgcagcgcc gtttcttttc
     1621 cgaggagcgc cacgacgtcg aagagcggcg cgccgacggt gacgccggtc agcgcgatcc
     1681 gcaccgggtg gatgagacgc gacgcagaga tcttcatctc ggctgcgagc tggcgcagcg
     1741 ccgcgtcgag ctcccgcgcg gtccacgcct ccaccttctc gtaggtcgcg atcagccgcc
     1801 cgatctcggc ggccgtctcg ggtctcacgt tcttgcgcat gccttccgtg tcgtactcct
     1861 ccgggtcctt cgcgtacacc gagagcgccc gccccatttc gatcgcggtg cgagcgcggg
     1921 tgcggtgaag ctcgaccgcc gcgagagccg cctcccgctc cggcattccc gccggcgcgt
     1981 aaggctcggc gcgcttcagt aactcttccg cgggcatgcg cgccatgtac tgcgcattca
     2041 cccacagcag tttttccgga tcgaagaccg agggcgacgc gccgatccgc tcgagcgaga
     2101 actcccgctc catctcttca cgcgtcagga tctcgcgatc gtcgccggga gaccacccga
     2161 gcagggcaag gaagttgacg agcgcctcgg gaaaaatgcc ctgctcccgc cactcctcga
     2221 cggccacggc accgtagcgc ttcgagagct tcttgcggtc ggtgccgaga atcatcccga
     2281 gatgcgcgaa cgccggcacc tccgcgccga gcgcgcgaaa gagcgcgacg tgtttcggcg
     2341 tattcgcaag gtgatcgtcg ccgcggagca cgtgcgtgat ccgcatgtcg gcgtcgtcca
     2401 cgcataccgt gaagtgatag agcgggtgtc cgtcggagcg cacgagcacg aagtcatcga
     2461 gggcgtcggc ggggaactcg acgcggcccc ggatcagatc gtcgacgacg atcggctcgt
     2521 ggggcatctt gaggcgcacg acgaacttct ccccggcgcg ggcgcggcgg tcgctctcgt
     2581 cgggtgcaag gtctcggccg gcgcccgagt accggtaaga gcgcccctcc gactcggctt
     2641 tcttgcgtgc cgcatccagc tcttcggggg tttcgaacgc gcgaaacgcc ttcccctccg
     2701 cgatcagccg atccgcggcg gcgcggtaga ggtcggagcg cttcgattgg tagtacgggc
     2761 cctcgtcgtg ctggagcccg agccaatcga gggctgacag gatctggccc gagagctcct
     2821 cgcgcgagcg ctcgacgtcg gtgtcctcga tgcgaaggat gtacgtcccc cgttcgcggc
     2881 gcgcgaagag gaggttgtag agcgccgtgc gcgcgccgcc gacgtggagg aatccggtcg
     2941 gcgatggcgc gaagcgaact cggggaccgt cggtgtttgc cagaacgccg catgctagct
     3001 tacgaaaagt cgtccgcaaa accgcacatg agtgacgtgg aaaggaccga gcctgtctcg
     3061 atgcgcctgc gcgcggacgt cgcgctcgtc ctggtcacgc tcgtctgggg gtcgtcgttc
     3121 gtcgttgtga agaacgtgct cgtcgaagcg ccgccgctcg cattcgtttt cttccgcttc
     3181 ctgatcgcga cggtcgtgtg tctgccgctc acgttccggc ggccgcgtag tcccgatctg
     3241 ctgcgagacg gcgccatcat cgggtctctg ctcgcggcgg gaatgatttt ccaggtgctc
     3301 ggacagaccg agacgtccgc ctcgaaggcg gcttttctca cgggtctgtc ggtcgtgctg
     3361 acgcctttcg cggcgttcct gcgaacccgg cagctcccgt cgctcgagaa cggcgtcgga
     3421 atcgcgctcg cggggatggg cttcttcctg ctcacgcttc cgcagggcgg ggagccgatc
     3481 aaccgcggcg acgtcctcgt gttcgcgtgc ggcgtcgtct tcgccttcta catcgtcgag
     3541 ctcggggtgc gcgcgggaag acacgacgcg agccggctca cggttatcca gctcgccgtg
     3601 gtcgccgccg tggcggggat ggtcgccgtc gtcttccgcg cggcgggagc ggccgtggag
     3661 agccgcccga tcgtgtgggg aggaacgttc ctgcgcagcg tcctctacct cgcgacgatc
     3721 ggcaccatcg ggaccttcct cacccagacg tgggcgcagc gccacatgtc gtcgacgcac
     3781 gcggcgatca tcttcgcgct cgagcccgtg tgggcggcgc tcttggcggc gtggctgctc
     3841 ggggagcggc tggagacggt gggctactgg ggggccgcgc tcgtgctcgc ggggatcgtg
     3901 gtgtcggagc tgcggctacg ctcccgatgc aagccctgat gtggcaagtg gagacctgac
     3961 cctcaatgtg gcaagtggag acctgacccc ctcaatcgag ggggaaagtc agggtatacg
     4021 cgaagcggcc gcggccgacc tccatcagga gcgtcgtgcg attgtagtca cgctggacga
     4081 tccggttgac gtcggcgacg gtcgtgaccc gggtcccgtt caagccgagg agcacgtccc
     4141 ccgactcgat tccgcggtcg gcggcgtcac cgttgcggtc gacgacgttg atgcgcaggc
     4201 cgccgcgcgc cgctgaaacc gtgatgccca gctgctcgcg aaggagctgg gatccgagat
     4261 cggccggcgg cgcctggccc tgcaccgtga tcgtccgctc gccgcccgag ccgcggagaa
     4321 ggagccgcat cggccgcccc ggtccccggg tcgagagcgc ggtctcgaac gcttcacgcg
     4381 agtcgatcgg cgtcccgtcg acggagacga tgagatcccc cgagcggact ccggcccggg
     4441 cggcgggcga ggcgggagtc acgctgcgga tccggaaccc tcccggccgc gcgagctcgg
     4501 cgttacccga cgtgagcgtc tggccgcgca cgccgatcca cacagcgcgc acttcgccga
     4561 agcgcacgag gtcgtccacg atccgccgcg cgcggtccac ggggatcgcg aagccgattc
     4621 cctgcgcgcc gccgatgatc gcggtcgcga cgccgatcat gtccccctgg atgttgacga
     4681 cgggtccgcc cgagttcccc gggttgatcg gcgcgtcgat ctgcacgaag tcggtgtaca
     4741 tccgctggtt ctctcccggg acgcttcggc cgagcgccga aacgacgccg gcggtgacgg
     4801 tgttcgagag gccgaacggg ttcccgatcg cgacgatcgt ctcgccgatc aggaggtccg
     4861 aggacgttcc gagccggatc gtcgggaggt ttccgcgcga gttgcggacg cgcagcacgg
     4921 cgaggtcgtt gtccgcgtcg gaccccacga cgtcgcattc gagctccttg ccgcttttgg
     4981 tcgtcgcgat gatgcgggag gcgccggaaa tgacgtggtc gttggtgaga atgatgccct
     5041 cgggccggat gatgactccc gagccgagcg actgcgagcg ccggggccgc gggtccaccc
     5101 cgaagaagaa ctcgtcgacg agcgagggac gccgctggac ggtctgctcc gccgagatgt
     5161 tgaccacggc gggagagacc ttttccacga cgtcgacgat ctcgctgcgg cgcgaaggca
     5221 acggagccgc cgcggccgcg ccgtgcgcgg cgattgcgag caagaccgcg agagtccgtt
     5281 tcacgtcagg cgaaaccgcc gtgcgaggcg cgttcattcc ggcgaaaaga aagccccact
     5341 ttcgcggggc atgaatcaaa cgatgaggag aggaagggcc cggctttacc gggtctggac
     5401 gtcgatgggc ttcttctccg catgcccgga cgccgtgtcg ccgctgatct tgatctgctt
     5461 cggcttcgcg tcctcgcgct tgggcatttc gacctcgagc agcccgctgt tgaaccgcgc
     5521 tcggatgttt tcgcgatcga cgttgttggg cagcgtgaag gaccggatga actgtccgta
     5581 ggaccgttcg acgcggtggt agttgcggcc gttcttctct tcttcgaact tgcggtcgcc
     5641 tcggagcgtc agaaggttgc cctccatctg gatctggacc tcgttctcct tgaagcccgg
     5701 gagctccgcg gtcagcgtga tcttctcctt gtcctcggcg atgtcgacgg gcggcatcca
     5761 ggcgcccagc ccgacttcct cttcccgctc gcgggccggc accgaaccga atgtccggtt
     5821 gaagcgatcc gcaaacgagc tcaactcacg atagggatcc cagcgaatca atgccatatg
     5881 cacctcctgg agttgtggaa cgacacaatt gacgcttaca cactgagcac tcgtggctca
     5941 gactccgttt cgatttccag actctctccg tccgtcgaga ccgtgacctg cgcggtgtct
     6001 ccgggcgcga gctcgttgcg aaggatcttt ccggcgagcg cgtcggagac ctccttctga
     6061 atgagccgct tcaaggggcg cgcgccgaac gtcgggtcgt atccgcggcc ggccaggtac
     6121 cgcttcgcct cttcgctcca ggtcagccgc acgccgcggt cggcgagcgt ccgcgccaga
     6181 gactggagct ggatctccac gatctcctgc gtctcttcgc gggtcagccg gtggaagacg
     6241 atgacgtcgt cgatccggtt gaagaactcc ggccggaaat ggcggcgcag cgtctcttcg
     6301 atgcgtgcct tcacttccgc ctcgttcatg tcctgcgcct cggcgatcca ctcggatccg
     6361 aggttcgacg tcatgatgag cacggtgttc ttgaaggaga cgacgcggcc cttgccgtcg
     6421 gtgagccgtc cgtcgtccag gacctggagc aggatgttga agacgtcggg gtgcgccttc
     6481 tcgatctcgt cgaagaggac gacggcgtaa ggacgacggc ggatcgcctc ggtcaggcgg
     6541 ccgccctcct cgtaaccgac gtatcccggg ggcgcgccga tcatccgggc gaccgaatgc
     6601 ttctccatgt actcggacat gtcgagacgg accatcgcct gctcgtcgtc gaagagaaac
     6661 tccgcgagag cccgcgcgag ctcggtcttg ccgacaccgg tcggtccgac gaaaaggaac
     6721 gacccgatcg ggcgccgcgg gtcggagagc ccggcgcggg agcggcgaac agcgtcggac
     6781 actgcccgca ccgcttcccg ctggccgacg atgcgctgcg cgaggcggtc ctccatgtga
     6841 aggagcttct gcgtctcggt ttcgagcatc cgggagacgg gcacgccggt ccagcgcgac
     6901 acgaccgccg cgatgtcctc ctcggtgacc tcctcgcgga gcatcggacg gccgcccttc
     6961 tgcaggtccg cgagacgcgt attgttcgcg gcgagctctc tctcg
//
LOCUS       DASWAV010000340         4502 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U2665
            k141_575759, whole genome shotgun sequence.
ACCESSION   DASWAV010000340 DASWAV010000000
VERSION     DASWAV010000340.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960316
            Sequence Read Archive: SRR10260007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 4502)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4502)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:47:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,929
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4502
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_575759"
                     /isolate="SMAG_U2665"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.36444 N 132.31889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(118..726)
                     /locus_tag="VF958_07015"
     CDS             complement(118..726)
                     /locus_tag="VF958_07015"
                     /inference="COORDINATES: protein motif:HMM:NF012383.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyltransferase family protein"
                     /protein_id="HEX9148896.1"
                     /translation="MSSDILKTLPARHGHFLLESGYHADRWFGLDALFVSPRELAPLV
                     TALAARLRPYGASAVCGPLVGGAFVAQALATELGVDFFYSEPVAMHSATGLFAAAYRL
                     PAAQRERVRGHRVALVDDVISAGSSIRATAAALKAAGATTVVVGSLLFLGTKALDYLA
                     RFGVPVETLGRQDFTIWEPAACPLCRIGAPLEDPMAPRVSGG"
     gene            828..1373
                     /locus_tag="VF958_07020"
     CDS             828..1373
                     /locus_tag="VF958_07020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010036903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9148897.1"
                     /translation="MPGSTWKNTALVVTLLSIGPAFAGAAPPVSGARPAAVATAFNRL
                     TALAGDWVAAEDGEMFKKGDVVARYTVTAGGSAVVETVFPGSPHEMVTVYHADGPDLV
                     LTHYCMEGNQPRMRARNAKGTRFDFAFDGGTNIDPRKDRHMNSASLELVGGDELVSVW
                     TEIEAGKPVFVAKSHLVRKPR"
     gene            1424..1783
                     /locus_tag="VF958_07025"
     CDS             1424..1783
                     /locus_tag="VF958_07025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002635973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HEX9148898.1"
                     /translation="MKYLLLIYENEGNFANVSEADQGRIFQEYMDYSRGIKKNGNYIG
                     GEALQPIATATTVRQRNGKTLTTDGPFAETKEQLGGFYLVEAKDLDEAIKLAGGIPAV
                     RTGSIEVRPIMPTPPVE"
     gene            1972..2346
                     /locus_tag="VF958_07030"
     CDS             1972..2346
                     /locus_tag="VF958_07030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016840615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HEX9148899.1"
                     /translation="MRFLSMVKSAENSGPPPQELMDAMGKLAEDATKAGVLVETGGLL
                     QSAAGARVRVSGGKITMTDGPFTEAKELVGGYAILEVKSKAQAIEETRRFMELHRQHW
                     PGWEGEAEIRQIFSPSDFGADG"
     gene            2339..2752
                     /gene="tsaA"
                     /locus_tag="VF958_07035"
     CDS             2339..2752
                     /gene="tsaA"
                     /locus_tag="VF958_07035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012525109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N6-threonylcarbamoyladenosine(37)-N6)-methyltransferase
                     TrmO"
                     /protein_id="HEX9148900.1"
                     /translation="MADPETYLLNPIGVVRSPLKNREAAPRQGSEGAPEASIELAPSV
                     LEGLQGISAGQEIIVITWLHESRRDVLKVHPRSDESVPLAGVFATRSPDRPNPLGLHR
                     VTVLEIAGRELKVAPLEAIDGTPVVDIKPVLRDGS"
     gene            2757..3596
                     /locus_tag="VF958_07040"
     CDS             2757..3596
                     /locus_tag="VF958_07040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018614796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-3' exonuclease H3TH domain-containing
                     protein"
                     /protein_id="HEX9148901.1"
                     /translation="MIVHLVDGTYELFRHFYGLRRFNDGEDPPFGAVIGVLHTVLEMI
                     ERGATHVGVATDHVIESFRNGIWSDYKTSAGVEPALLAQFHPLEEALAAMGVAVWPMT
                     ELEADDGLASAARIASSSDRVERVCIWTPDKDLAQCVRGNRIVQVDRRGQKIRDDKGV
                     REKFGVEPFLIPDFLALVGDAADGYPGIPRIGRITAARLLNRHGIIEDFPSDVLGDGR
                     NLALLFKDLATLRTDAPLFRDVEELRWRGPTDAFAGCAGYLGDARLLERSLRAAVFQE
                     TRT"
     gene            3593..4018
                     /locus_tag="VF958_07045"
     CDS             3593..4018
                     /locus_tag="VF958_07045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012525678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HEX9148902.1"
                     /translation="MTEPNLAAKWASWAPQLRSIFRIVAAAMFIVAGTIKVFAFPIGV
                     PPNGGTVPVMSEAWIGGVLELVGGGLLLVGLFTRPVAFVLSGEMAVAYFQFLFPQSFW
                     PHVNNGVPAVLYCFLWLYFSAAGAGPWSLDALRGKSVGS"
     gene            4043..4402
                     /locus_tag="VF958_07050"
     CDS             4043..4402
                     /locus_tag="VF958_07050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007364093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1428 domain-containing protein"
                     /protein_id="HEX9148903.1"
                     /translation="MARYVDGYVLPVPKKNVEAYRRLAQIGARIWREHGALEFRECVG
                     EDLSVKWGVPFPRGIKAKRGETVVFSWIVFKSRAHRDRVNAKVMKDPRMTSMDMKSMP
                     FDARRMLYGGFEVLVES"
BASE COUNT          817 a         1439 c         1508 g          738 t
ORIGIN      
        1 caaccagggc gaagccgaag aagagcgcca gcggcggaac gagccgccgg cgagccgcag
       61 tccggtgagt cacgtttggg ttgcccgagg aaacgatacg ccgcggcaat atgccgttca
      121 accgccggaa actcgggggg ccatcgggtc ctcgagaggc gcgccgatgc ggcagagcgg
      181 acacgccgcc ggttcccaga tcgtgaaatc ctgtcggccg agcgtctcga cgggcactcc
      241 aaagcgagcg agatagtcga gcgccttcgt ccccagaaag agcagtgacc cgaccacaac
      301 cgtcgtcgcg ccggcggcct tgagcgccgc cgcagttgcc cggatcgacg agccggcgct
      361 gatcacgtca tcgacgagcg cgacgcgatg tcctcgcacc ctttcgcgct gcgcggccgg
      421 caggcggtaa gcggccgcga acaagccggt cgcggagtgc attgcgacag gttcgctgta
      481 gaagaagtcc actcccagct cggtcgcgag agcctgagcc acgaacgcgc ccccgacaag
      541 ggggccgcac accgcagagg cgccgtaggg tcgcagtcgc gcggcgagtg ccgtgacgag
      601 cggcgcgagc tctcgcggac tcacgaacag cgcatcgagg ccgaaccacc ggtcggcgtg
      661 gtagcccgat tccaggagga agtggccgtg gcgggcgggc agcgtcttca ggatgtcgga
      721 agacatcgga tcctcgaatt tagctcgaat ggatccgtcg atttcgggcg aacccgttcg
      781 actggaggtt gagagcgcat ggcgcgcctc atccgaaagg agacgatatg cccggaagta
      841 cctggaagaa cacggccctg gtggtgacgc tcctgtcgat cggcccggct ttcgcgggcg
      901 ccgcgcctcc cgtttccggc gccagaccgg ccgcggtcgc gactgccttc aaccgcctca
      961 cggcgctcgc cggggactgg gtcgccgccg aggacggcga gatgttcaag aagggggacg
     1021 tcgtcgcccg ttacacggtc accgcgggtg gctctgccgt cgtcgagacg gttttccccg
     1081 ggagcccgca cgagatggtc acggtctacc atgccgacgg tccggatctc gtcctcaccc
     1141 actactgcat ggaaggcaac caaccgcgaa tgcgcgcccg gaacgccaaa ggcacccgtt
     1201 tcgacttcgc gttcgacggc ggtacgaaca tcgacccgag aaaggaccga cacatgaact
     1261 cggcgtccct cgaactcgtc ggtggggatg agctcgtgag cgtctggacc gagatcgaag
     1321 ccggcaagcc ggtgttcgtc gccaagtcac acctcgtccg caaaccgcgc tgaccggttc
     1381 cacgtaagaa acgagaacac gatcgatcag aaaggacacg acgatgaaat acctgttgct
     1441 gatttacgaa aacgagggga acttcgcgaa cgtctcggag gccgaccagg gcaggatctt
     1501 ccaggagtac atggactact cgcgggggat caagaagaac gggaactaca tcggcggcga
     1561 ggcgctgcag cccatcgcga cggcgacgac agtgaggcag aggaacggaa agacgctcac
     1621 gacagatgga ccgttcgcgg aaacgaagga gcagctcggc ggcttctacc tcgtggaggc
     1681 gaaagacctg gatgaggcga tcaagctcgc gggcggcatc cccgccgttc gaaccggctc
     1741 gatcgaggtg cgcccgatca tgccgacccc gccggtcgag taacgagggt tccccgccat
     1801 tcggtctttc tcgagcgtgc gccgcccggc ggatgaaact gccgggcggc cgatgttctc
     1861 gcatccggat ttttcgctcg ctcggttttc ggcggccggc ggagaatacc cgcgcggcgt
     1921 gtcgatcccg ggctccgtcg ttcgtcgcga gagtagaaag gagatccgac gatgcgattc
     1981 ctgagcatgg tcaaatcggc cgaaaactcc ggccctcccc cccaggagct catggacgcc
     2041 atgggcaagc tcgccgaaga tgcaacgaag gccggtgtcc tcgtcgaaac cggcggactt
     2101 ctccagagcg cggccggcgc gcgcgtccgg gtgtccggag gaaagatcac catgaccgac
     2161 ggccctttca ccgaagccaa ggagctagtc ggcggctacg ccattctcga ggtgaagtcg
     2221 aaggcgcagg cgatcgaaga gacgcggcgt ttcatggaac ttcatcggca gcactggccg
     2281 ggctgggaag gagaagccga gatccgccag attttcagcc cttcggactt cggcgccgat
     2341 ggctgatccc gagacgtatc tcttgaatcc gatcggcgtc gtccgctcgc ccctcaagaa
     2401 tcgcgaagcg gcgccccgcc agggaagtga aggagctccg gaggcgtcga tcgagctggc
     2461 gccgtcggtg ttggagggac tgcagggaat cagcgctggc caggaaatca tcgtcatcac
     2521 gtggcttcac gagtcccgac gcgacgttct gaaggtgcac ccgagatcgg acgagagcgt
     2581 tccgctcgcg ggagtcttcg cgacgaggtc tccggaccgg ccgaatcccc tcgggttgca
     2641 ccgcgtgacg gttctcgaga tcgccgggcg cgagctgaag gtcgcgccac tcgaagccat
     2701 cgacggcacg ccggtggtcg acatcaagcc cgtgctgcgc gacggcagct gagagcatga
     2761 tcgtccatct cgtcgacggc acttacgagt tgttccgtca cttctacggc ctgcgccgct
     2821 tcaacgacgg cgaggatccg cccttcggcg ccgtcatcgg cgtgctgcac acagtgctcg
     2881 agatgatcga gcggggggcc actcacgtcg gcgtggcgac cgatcacgtc atcgagtcct
     2941 tccggaacgg gatctggtcg gactacaaga cgtcggccgg cgtggagccc gcgctcctcg
     3001 ctcagtttca cccgctcgaa gaagcgctcg ccgccatggg cgtggcggtg tggccgatga
     3061 ccgagctcga ggccgatgac gggctcgcgt ccgcggcgcg gatcgcctcg tcgagcgatc
     3121 gggtcgagag agtctgcatc tggactcccg acaaggacct cgcgcagtgc gtccgcggca
     3181 atcggatcgt tcaggtcgac cgccgggggc agaagatccg cgatgacaaa ggagttcgcg
     3241 agaagttcgg ggtcgagcct ttcctgatcc ccgacttcct ggctctcgtc ggagacgcgg
     3301 cggacgggta tcccggcatt ccgaggatcg gtcggatcac ggcggcccga cttctcaacc
     3361 ggcacgggat catcgaggac ttcccgtcgg acgtcctggg ggacggccgc aatctcgcgc
     3421 tgttgttcaa ggatctggcg acccttcgga ccgacgcgcc gctcttccgc gacgtcgaag
     3481 agctgcgctg gcgcggcccc accgacgcgt tcgccggatg cgcggggtac ctgggggacg
     3541 ctcgtctgct cgagcgctcc ctcagggcag cggttttcca ggagacacgc acgtgaccga
     3601 accgaacctc gcagcgaaat gggcgtcgtg ggcgccgcag ctgcgaagca ttttccgcat
     3661 cgtggccgcg gccatgttca tcgtcgccgg cacgatcaaa gtcttcgcct tcccgatcgg
     3721 agtcccgccc aacggcggca ccgttccggt aatgtcggag gcatggatcg gcggcgttct
     3781 cgagttggtt ggtggcgggt tgctcctcgt cggtcttttc acccgccccg tcgccttcgt
     3841 cctctcgggc gagatggctg tcgcgtactt ccagttcctg ttcccgcaga gcttctggcc
     3901 gcacgtgaac aacggcgttc cggccgtgct ctactgtttc ctctggctct acttctcggc
     3961 cgccggcgcg ggtccgtgga gcctggacgc gctccggggg aagtcggtcg gctcatagtt
     4021 cgcaaacacg aggaggatcc gcatggcccg ctacgtcgac ggttacgtgc tgccggttcc
     4081 gaaaaagaac gtggaagcct accgacgcct cgcacagatc ggggcaagga tctggcgcga
     4141 gcacggcgct ctggagttcc gggagtgcgt cggcgaggac ttgagcgtga agtggggagt
     4201 gccgtttccg cgcgggatca aggcgaagcg cggcgagacc gtcgtcttct cgtggatcgt
     4261 cttcaagtcc cgcgcgcatc gtgatcgggt caacgcaaaa gtgatgaagg acccgcgcat
     4321 gacgagcatg gatatgaagt cgatgccttt cgatgcccgc cgaatgctct acggcgggtt
     4381 cgaggtgctc gtcgaatctt gaacccgacc gtctgacgct cgtacaaccc ggaatggccg
     4441 accccgcgcc caagctcgca cagcggttcg atcgctcgat cgtcgaggga cccatcggcc
     4501 cc
//
LOCUS       DASWAV010000341         2764 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U2665
            k141_178748, whole genome shotgun sequence.
ACCESSION   DASWAV010000341 DASWAV010000000
VERSION     DASWAV010000341.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960316
            Sequence Read Archive: SRR10260007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2764)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2764)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:47:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,929
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2764
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_178748"
                     /isolate="SMAG_U2665"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.36444 N 132.31889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..411
                     /locus_tag="VF958_07055"
     CDS             <1..411
                     /locus_tag="VF958_07055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite reductase, copper-containing"
                     /protein_id="HEX9148904.1"
                     /translation="AAGLREDPEFIVFNGGFGSLLGQRALQAETGQTVRIFFANAGPN
                     KISSFHVIGEIFDRVYREGDLVSPPARYVQSTLVPAGGSTVVEFQTLVPGRYILVDHA
                     IFRTEKGAIGHLNVIGQPRPDIYKGSGEPGESGH"
     gene            411..749
                     /locus_tag="VF958_07060"
     CDS             411..749
                     /locus_tag="VF958_07060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010940899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEX9148905.1"
                     /translation="MTTGSDGSAELAGVASQSDVVQYQTGAVVSRTLLKSPGGTVTAF
                     AFDAGEGLSEHTAPFDALVLMVEGEAEISISASPHRLHAGQLLKLPARQPHAVRAITR
                     FKMFLVMIKA"
     gene            836..1471
                     /locus_tag="VF958_07065"
     CDS             836..1471
                     /locus_tag="VF958_07065"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020549884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HEX9148906.1"
                     /translation="MAEYALPDLRYDYGSLEPHISGRIMELHHDKHHAGYVKGANLAL
                     ERLDEARHKDDFGNVGALEKLLAFNLSGHVLHSLFWQNLTPKGGGEPQGELASAIQRD
                     FGSFAAFKKQLTQVAGTIMGSGWAALVWEPIGQRLLTAQIYDHQSNITQAGVPLMVLD
                     AWEHAFYLQYQNRKTEFFDAIWSVWNWQDVADRFKAAKGLNVAVVGATAKK"
     gene            1599..>2764
                     /locus_tag="VF958_07070"
     CDS             1599..>2764
                     /locus_tag="VF958_07070"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HEX9148907.1"
                     /translation="MGEVFLAEDTRLGRKVALKLLPEGLLADREMRRRFAQEARTASA
                     LNHPNIVTIYDIGSADGRDFLAMEYVEGQSLRDVLAAGQLDIRRSLDLAAQAASALAA
                     AHETGIVHRDIKPENLIVNRAGQLKILDFGLAKLVERQTAPLLASSLETLAETPQPTS
                     EGRTGTGVILGTVSYMSPEQARGQPVDHRTDVFSLGLVLYEALTGARPFSGKSAVETL
                     HAIINEEPVSAVERNPRVPPQAVDILGKALAKDPAERYQHAADFALDLRRLARAPATP
                     SLAMAQASRPRSWTWAIAVAALLLGVAAGFVLFRLSAPKDASSLADVTVTPLTNDPGY
                     EGEPTFSPDGETVAYVSDRTGNFDIFLKQISGGPDVNITNSPADDVQPAFSPDG"
BASE COUNT          510 a          920 c          880 g          454 t
ORIGIN      
        1 gccgccggat tgcgcgaaga tcccgagttc atcgtcttca acggaggctt cggctctctg
       61 ctgggacagc gagccctgca ggcggagacg ggacagaccg tccggatatt cttcgcgaac
      121 gcgggaccga acaaaatctc gtcgtttcac gtgatcggcg aaatcttcga ccgcgtctat
      181 cgggaaggag acctggtcag ccctccggct cgctacgtcc agagcactct cgtccctgcg
      241 gggggatcga cggtcgtcga gtttcaaact ctcgttccgg gccggtacat actcgtggac
      301 cacgcgatct tccgcaccga gaagggggcg atcggtcact tgaacgtcat cgggcagccc
      361 cgacccgaca tctacaaggg gtccggcgag cccggagagt caggccactg atgacgacag
      421 gatctgacgg ttcggcggag ctggccggtg tcgcgtccca gtcggacgtg gtccagtatc
      481 agacgggcgc ggtcgtgagt cggaccctgc tcaagagtcc aggagggacc gttacggcgt
      541 tcgccttcga cgcgggcgag gggctctccg agcacacggc gccgttcgat gccctcgtgt
      601 tgatggtcga gggagaggcc gagatctcga tctccgcaag tcctcaccgt ctccacgcgg
      661 gtcagctcct gaagcttccg gcgcggcagc ctcacgccgt ccgggcgatc acgaggttca
      721 agatgttcct cgtcatgatc aaggcgtgag cggttgggcg gcgcccgttc ggtcggacgc
      781 gtcgcaagcc cgggcacgat ttctgataga aatgacgact aaggaggacc ctcccatggc
      841 tgagtacgcc ctgcccgatc tccgttacga ctacggttct ctcgagcccc acatctcggg
      901 caggatcatg gagctccacc acgacaagca ccatgccggc tacgtcaagg gagcgaacct
      961 ggccctcgaa aggctcgacg aggcgcgcca caaggacgac ttcgggaacg tcggagcgct
     1021 cgaaaagctg ctcgcgttca acctctccgg gcacgtcctg cactctcttt tctggcagaa
     1081 cctgactccc aaagggggcg gggaaccaca gggcgagctc gcgagcgcga tccaacgaga
     1141 cttcggaagc ttcgcggctt tcaagaagca gttgacgcag gtcgccggca cgatcatggg
     1201 ctccggctgg gccgcgctcg tctgggaacc gatcggccag cgcctcctga cggcccagat
     1261 ctacgaccac cagtccaaca tcacgcaggc gggagtcccg ctgatggtcc tggatgcgtg
     1321 ggagcacgcg ttctatctcc agtaccagaa ccgcaagacg gaattcttcg atgcgatctg
     1381 gagcgtgtgg aactggcagg acgtcgcgga tcgtttcaag gccgcaaagg gattgaacgt
     1441 cgcggtcgtc ggggcaaccg ccaagaaata gacgtctccc tctgcagaag cgggcgggct
     1501 ggtaagctct gaccacgatt cccgaaggac cgagctccgg cacgaagagg gcttccctct
     1561 cccactacga gctcctccgc aagctcgggc aagggggaat gggagaagtc ttcctcgcgg
     1621 aagacacccg gctcggacgc aaggtcgccc tgaagctgct ccccgaagga cttcttgccg
     1681 acagggagat gcgccgccgc ttcgcccagg aggcgaggac ggcctccgct ctcaaccacc
     1741 cgaacatcgt cacgatctac gacattggct cagccgacgg ccgggatttc ctcgcgatgg
     1801 agtacgtcga ggggcagagc ctgcgggacg ttctggcggc cggacagctc gacatccgcc
     1861 gctcgctcga cctggcggcg caggcggcgt cggccctggc ggccgcgcac gaaaccggga
     1921 ttgttcaccg cgacatcaag ccggagaacc tgatcgtcaa ccgggctggg cagctcaaga
     1981 tcctcgattt cggtctcgcg aagctggtcg aacgacagac ggcgccgctg ctggcgagca
     2041 gcctcgaaac cctggcggag acgccgcagc ccacctcgga gggacgcacc ggaaccggcg
     2101 tgatcctcgg gaccgtgtcg tacatgtcgc cggagcaggc gcggggccag cccgtggacc
     2161 accgcacgga cgtcttctcg ctgggtctcg tcctctacga ggcgttgacg ggcgcccgcc
     2221 cgttctccgg caagagcgcc gtcgaaacgc tccacgccat catcaacgaa gaaccggttt
     2281 cggcggtcga gcgcaacccg agggttccac cgcaggccgt ggacatcctc ggaaaggctc
     2341 tcgcgaagga cccggccgag cgttaccagc acgccgccga tttcgcgctc gacctgcgcc
     2401 gcctcgcccg cgcaccggcg actccgtctc tcgcaatggc ccaggcctcc cgcccgagaa
     2461 gctggacctg ggcaatcgcc gttgcggcgc ttcttctggg agtcgccgcg ggtttcgtcc
     2521 ttttccggct gagtgcgccg aaggatgcat cctctctcgc cgacgtcacg gtgacgccgc
     2581 tcacgaacga tccgggatac gaaggcgagc cgacgttctc gccagacggc gagacggtcg
     2641 cttacgtttc cgaccgcacc gggaatttcg acatcttcct gaagcagatc tccggcggcc
     2701 cggacgtcaa catcaccaac agtcccgcgg acgacgtcca gcccgcgttt tcgccggacg
     2761 gaaa
//
LOCUS       DASWAV010000342         4700 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U2665
            k141_777314, whole genome shotgun sequence.
ACCESSION   DASWAV010000342 DASWAV010000000
VERSION     DASWAV010000342.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960316
            Sequence Read Archive: SRR10260007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 4700)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4700)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:47:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,929
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4700
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_777314"
                     /isolate="SMAG_U2665"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.36444 N 132.31889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            231..1334
                     /gene="dinB"
                     /locus_tag="VF958_07075"
     CDS             231..1334
                     /gene="dinB"
                     /locus_tag="VF958_07075"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002772945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HEX9148908.1"
                     /translation="MSGAFRRILHVDMDAFYASVEQRDNPALRGRPVAVGGSPESRGV
                     VAAASYEARAFGVRSALPMSRAVRLCPDLVIVRPDFGRYGEVSRQVFAIFREVTPLVE
                     PLSLDEAYLDVTENAWGEPLAVDVARRIKAHIREQTQLTASAGVAPNKFLAKIASGWK
                     KPDGLTVISPDRIERFLAGLPVDALWGVGPVTARKLRAVGIERLVDVRKAGEEVLRQA
                     VGSWAEGLRELAFGRDERPVTPNHERKSIGCEETFARDIRDLEEIRRQLEALARGAAA
                     ALTHRRLLARTVVVKLRYSNFETITRSETRQPPTCSEEEIVARAVALLEKTEAGKRPV
                     RLLGASLHGLAAEPDAPPEPRGAREPQLALPVE"
     gene            1339..2517
                     /locus_tag="VF958_07080"
     CDS             1339..2517
                     /locus_tag="VF958_07080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015615452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxide hydrolase"
                     /protein_id="HEX9148909.1"
                     /translation="MIEPFRIAVPEEVLRDLRERILRTRWPDEIRGSGWEYGTSLHYL
                     RELLDYWTRGFDWRAQETFLNSFHHFRVAVDGQGLHFLHERGRGPAPLPLLLPHGWPS
                     SFYEMTKLVPLLTDPEAHGGDAADAFDVVVPSLPGCGFSERPRDPGMHKTRMAELFAD
                     LMTAVLGYSRFGARAGDIGAGVVSLLSLDYPERVIGIHLSDIYRPYLGPGARMFTDAE
                     RRFFEEERLWAEREGAYDHVQATKPQTLGYGLADSPAGLAAWIVEKFRSWSDCGGDVE
                     RRFTKDELLTNLTLYWVTNTINSANRLYFERDREPRPLGRDDRIAVPTAVTIFPGDID
                     HPPREWAERVYDVVRWTEMPRGGHFAAFEEPELLAEDIRDFFRPLRRSEAQKGPNRVQ
                     "
     gene            complement(2482..3114)
                     /locus_tag="VF958_07085"
     CDS             complement(2482..3114)
                     /locus_tag="VF958_07085"
                     /inference="COORDINATES: protein motif:HMM:NF024811.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HEX9148910.1"
                     /translation="MLKAVLFDWDGTLLDSAEAGLRSYERLFASYGIAYDRARFAETY
                     SPNWHLTYERIGLARDKWDEADAAWLEIYASEECGLVAGARDALALLETAGVRTGLVT
                     SGSRSRVLRELAALDLETRFRTVVCSEDVSRKKPHPEALHLGLERLAVSPEAAAYVGD
                     SPEDVGMARAAGVFSVGVAGGFPNREALRGSGPDLFSEDLLNAIRALLSF"
     gene            complement(3299..>4700)
                     /locus_tag="VF958_07090"
     CDS             complement(3299..>4700)
                     /locus_tag="VF958_07090"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012546.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HEX9148911.1"
                     /translation="DLLPGSKRLFSLQEGSGSFDVSPDGAWIAFEANSTPEPYEKLNR
                     DVFLVPTAGGEVRNLTTANPADDTDPVFSPDGKSIAYGVEVKADGWPDRTRLAVMDVA
                     SGRTTVLTEGFDVSAGGWAWSHDGRTIVFDAESRARTNLYAVPAKGGVPREIRHGGTT
                     SGAEVAPDGQVVFQFHSLTRPPELAVVRIDGKGSRTLTHVNDDLVSRIDWGRVDDLTF
                     SGADGDEVQMFVVYPPGFDAKKKYPLVHMIHGGPIGTFGDGFSFRWNPQAFAAPGYVV
                     PMVNFHGSSSFGQKWVESILGAHPDKPFTDVMKATDFLIAKGFVDENRMAAAGGSYGG
                     FLVDWIEGHTDRFKALVSHAGVYSLLGQSASDAYYGRQHSYGGYPFTNLENIERWSPD
                     RYAKNFNTPMLVIHGERDFRVPVTQGLALYGVLKTKGVPARLVYYPDENHWVLKGQNS
                     KHWYGEVLGWLARWLK"
BASE COUNT          771 a         1597 c         1577 g          755 t
ORIGIN      
        1 ggatcgcgcc gtccgcgacc gggatgtcgt aagactttcc cgtccggtga tcgacgatcg
       61 tgacggcgtc gcgcttgtcc gtcagtggca cgggaggtac ggaagctcga aaagtatcaa
      121 ctgtagtcga cattgtcata cacctttcgt cgaaatttct gggccagtct agaggtcggt
      181 ccccgggcgt cgcctgatgc atttccctca agcggagaga gaatgacccc gtgtccgggg
      241 ccttccggcg gatccttcac gtcgacatgg acgcctttta tgcgtcggtg gagcaacgag
      301 ataaccccgc cctgcgcggc aggccggtcg cggtgggggg cagtcccgaa agccgtggcg
      361 tggtcgcggc cgcgagctac gaggcgcgcg cgttcggagt ccgctccgcg ctccccatgt
      421 cccgcgccgt gcggctgtgc ccggacctcg tcatcgttcg gccggacttc ggcaggtacg
      481 gcgaggtttc gcggcaggtc ttcgccatct tccgcgaggt gacgccgctc gtcgagccgc
      541 tctcgctcga cgaggcctac ctcgacgtca ccgagaacgc ctggggggag cctctggcgg
      601 tggacgtcgc gaggcggatc aaagcgcaca tccgcgaaca gacgcaactc accgcatccg
      661 ccggcgtcgc tcccaacaag ttcctcgcga agatcgcttc cggctggaag aagccggacg
      721 gcctcacggt catctcgccc gatcggatcg agagattcct cgcggggctg cccgtcgacg
      781 cgctgtgggg cgtcgggccg gtgacggcgc ggaagctgcg cgcggttgga atcgaacgtc
      841 tcgtcgacgt gcggaaggcc ggcgaggaag tcctgcggca ggccgtcgga agctgggcgg
      901 agggacttcg cgagctcgcg ttcggacggg acgagcgacc ggtgacgccg aaccacgagc
      961 gcaagtcgat cggctgtgaa gagacgttcg cgcgggacat tcgggacctc gaggagatcc
     1021 gccggcaact cgaggcgctc gcccgcggcg ctgcggctgc gctcacgcac cggcgcctgc
     1081 tggcgcgcac cgtcgtggtc aagctgcgct actccaactt cgagacgatc actcgaagcg
     1141 agacccgcca gccacccacg tgctcggaag aggagatcgt cgcacgggcg gtcgcgctgc
     1201 tcgagaagac cgaagccggg aagcgccccg tgcgcctcct cggcgcctcg ctgcacggtc
     1261 tggccgccga gccggacgcg ccgccggagc cccggggcgc gcgggagccg cagctcgccc
     1321 tgccggtcga gtgatggggt gatcgaaccc tttcgaatcg cggttccgga ggaggtcctg
     1381 cgagaccttc gggaacggat tctccggacg cgctggcccg acgagatccg cggcagcggc
     1441 tgggagtacg ggaccagcct ccattacctc cgcgagctcc tcgactactg gacgcgggga
     1501 ttcgactggc gtgcgcagga gacgttcctg aactcgtttc accacttccg ggtcgccgtg
     1561 gacggtcagg gccttcactt ccttcacgag cgcggacgcg ggccggctcc gctgcccctc
     1621 cttcttcccc acggctggcc gagctcgttc tacgagatga cgaagctcgt gccgctcctg
     1681 accgaccccg aagcgcacgg cggggacgcg gccgacgcat tcgacgtcgt cgtgccctcg
     1741 ctgccgggct gcgggttctc cgagcggccg cgcgaccccg ggatgcacaa gacccggatg
     1801 gcggagctct tcgcggacct catgaccgcg gtgctcggat attcgcgctt cggagcgcgc
     1861 gccggagaca tcggagccgg cgtcgtctcg ctgctctcgc tcgattaccc cgagcgcgtg
     1921 atcggaatcc acctctccga catctatcgc ccgtacctcg gacccggtgc ccggatgttc
     1981 acggacgccg agcgccgctt cttcgaagaa gagcggctgt gggccgagcg ggagggagcg
     2041 tacgaccacg ttcaggcgac gaagccgcag acgctcggat acggcctcgc cgactcgccc
     2101 gccgggctcg ccgcgtggat cgtggaaaag ttccggagct ggagcgactg cggcggcgac
     2161 gtcgagcgcc gcttcacgaa agacgagctt ctgaccaacc tcaccctcta ctgggtcacc
     2221 aacacgatca actccgcgaa ccggctctac ttcgagcggg accgcgagcc gcgaccgctc
     2281 ggacgggatg accgcatcgc cgttccgacg gccgtgacga tcttcccggg ggacatcgat
     2341 cacccgcctc gcgagtgggc cgagcgcgtc tacgacgtcg tgcgctggac cgagatgccc
     2401 cggggcggac acttcgcggc gttcgaggag cccgagctcc tcgcggagga cattcgcgac
     2461 ttcttccgac cgctgcggag gtcagaagct cagaagggcc cgaatcgcgt tcagtaggtc
     2521 ctccgagaag aggtccggcc ccgagccccg gagagcctcg cggttcggaa atccgccagc
     2581 gactccaact gaaaaaacgc ccgcagcgcg cgccatcccg acgtcctccg ggctgtcccc
     2641 gacgtaggca gccgcttccg gcgatacggc gagccgttcg aggcccaggt gcagtgcctc
     2701 cgggtggggt ttctttcgcg acacgtcctc cgagcagacg acggtccgaa aacgcgtctc
     2761 gaggtcgaga gccgcgagtt cgcgaagcac tctcgagcgc gagccgctcg tgacgagacc
     2821 ggtccgaaca ccggccgtct ccagaagggc gagcgcgtcc cgcgccccgg cgacgaggcc
     2881 gcactcctcc gacgcgtaga tctcgagcca cgccgcgtcg gcctcgtccc acttatcgcg
     2941 tgcgagcccg atcctctcgt acgtgaggtg ccagtttggc gagtacgttt cggcgaagcg
     3001 ggcgcgatcg taggcgattc cataggaagc gaagaggcgc tcgtacgacc gcaggccggc
     3061 ctccgccgaa tcaaggaggg tcccatccca gtcgaacaaa acggccttca gcatcgacac
     3121 tcggtcgcat cgcaaagagt gtccgaggtc gtcgttcgcg gcgcgagtag tcccccgaag
     3181 ggaggaggag gctcgcgccg cgggagcggg agcgagtgag ggggcaggcg agcggtcgag
     3241 ccgggactgt cgagccgctc gccggtgcga agcagctccc gagcgagcga aagcgacgtt
     3301 acttcaacca cctcgcgagc cagccgagca cctcgccata ccagtgcttc gagttctgcc
     3361 ccttcaggac ccagtggttc tcgtcggggt agtagacgag ccgcgccggt acgcccttcg
     3421 tcttcagcac gccgtacaac gcgagcccct gagtcaccgg gacgcggaag tcgcgctcgc
     3481 cgtggatcac gagcattggc gtattgaagt tcttcgcgta acggtccggg ctccagcgct
     3541 cgatgttctc caggttcgtg aagggatatc cgccgtacga gtgctgccgc ccgtagtacg
     3601 cgtccgacgc ggactgtccg agcaggctgt agacgccggc gtgcgagacg agcgctttga
     3661 aacggtcggt gtgcccctcg atccagtcca ccaggaaccc tccgtacgac ccgccggcgg
     3721 cggccatgcg gttctcgtcc acgaaaccct tcgcgatcag aaagtccgtg gccttcatga
     3781 cgtcggtgaa cggtttgtcc gggtgggcgc ccaggattga ctcgacccac ttctgcccga
     3841 agctcgaaga cccgtggaag ttgaccatcg ggacgacgta gccgggcgct gcgaacgcct
     3901 gcgggttcca acggaagctg aagccgtctc cgaacgtccc gatcggcccg ccgtggatca
     3961 tgtgcacgag cggatatttc ttcttcgcgt cgaaacccgg agggtagacg acgaacatct
     4021 ggacttcgtc gccgtcggcg ccggagaacg tcaggtcatc gactcttccc cagtcgatcc
     4081 gtgagacgag gtcgtcgttg acgtgggtga gagtgcggga gcctttcccg tcgattcgta
     4141 cgacggcgag ttcggggggc cgggtcagcg agtgaaactg aaagaccacc tgaccgtccg
     4201 gcgcgacttc ggcgcccgag gtcgtgccgc cgtgccggat ttccctgggg accccccctt
     4261 tcgccgggac cgcgtacaga ttcgttcttg cccgcgactc cgcgtcgaag acgatcgtcc
     4321 ggccgtcgtg tgaccaggcc cacccgcccg cggacacgtc gaagccctcg gtgagaaccg
     4381 tcgtgcggcc gctcgcgacg tccatcacgg caagacgcgt gcgatcgggc cagccgtccg
     4441 ccttcacctc gacgccgtag gcgatcgatt tcccgtccgg cgagaaaacc gggtcggtgt
     4501 cgtcggccgg gttcgccgtc gtcaggttcc ggacctcccc ccccgcggtc ggcacgagaa
     4561 agacgtcacg gttcagcttc tcgtagggct cgggcgtcga gttcgcttcg aaagcgatcc
     4621 aggcaccatc gggcgacacg tcgaagctcc ccgacccctc ctggaggctg aacaggcgct
     4681 tcgagcccgg caggagatcg
//
LOCUS       DASWAV010000343         2458 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U2665
            k141_376044, whole genome shotgun sequence.
ACCESSION   DASWAV010000343 DASWAV010000000
VERSION     DASWAV010000343.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960316
            Sequence Read Archive: SRR10260007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2458)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2458)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:47:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,929
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2458
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_376044"
                     /isolate="SMAG_U2665"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.36444 N 132.31889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..303
                     /locus_tag="VF958_07095"
     CDS             <1..303
                     /locus_tag="VF958_07095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014785378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminofutalosine synthase MqnE"
                     /protein_id="HEX9148912.1"
                     /translation="LLLDNVPHVKAYWVQIGTKLAQVALFFGADDLVGTVVDETITHA
                     AGATTASGMTRPEFERLIRESGREPFERDAEYRRVARSDRPVPAAPPSTETVSVPA"
     gene            318..1313
                     /locus_tag="VF958_07100"
     CDS             318..1313
                     /locus_tag="VF958_07100"
                     /inference="COORDINATES: protein motif:HMM:NF022818.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3179 domain-containing (seleno)protein"
                     /protein_id="HEX9148913.1"
                     /translation="MGALFLANVLASACLASGYRTKPRVAIVDGDPVVQMAEPGQFRS
                     ATNPRWAAADDQTDAPKHHQRVLGLEIHGVRIAYPIGLLDRAEVVNDGLDGVAWVAAR
                     CALTHVAAVYDRVVDGRTLTFENSGALWRDTLVLRDRETGTYWTAATGVALSGPLAGR
                     RLRSIPAAYTTALAWQRAFPDSRWADLGLPTSVPLSMKIYGASPWQGVSREKTRDRRH
                     PAKKEFLEVAIGREALAFTRGEIRRRRTAVATVGGETIRLEWDAATQAAQAWAGEGAG
                     RRELAVVPMYWFALDRHFDAIRSLAVGESERGQSRPRFRRRRISDRPAASRSPAA"
     gene            complement(1273..1938)
                     /locus_tag="VF958_07105"
     CDS             complement(1273..1938)
                     /locus_tag="VF958_07105"
                     /inference="COORDINATES: protein motif:HMM:NF015961.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HEX9148914.1"
                     /translation="MGSRILSRLAGALLYLHRSTLRVERMHFERYLGLKARGVPILFT
                     LWHGRMFLSIQAHRHEGIVTMASRSKDGEIIAGWLERNGYFVVRGSSTRGGSEALREM
                     VRRIRGGENAALTVDGPTGPPRQVQPGIVQLARLTGGWILPVTSSSSRPRFLSSWDRY
                     LLPMPFSRNVVLYGEPFPIDERDSDEEAQERIGAALDEATREADRAAGITPPEIWTLP
                     ADR"
     gene            complement(1950..>2458)
                     /locus_tag="VF958_07110"
     CDS             complement(1950..>2458)
                     /locus_tag="VF958_07110"
                     /inference="COORDINATES: protein motif:HMM:NF024748.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008800 - beta-lactamase activity
                     [Evidence IEA];
                     GO_process: GO:0030655 - beta-lactam antibiotic catabolic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HEX9148915.1"
                     /translation="RNAVNRFFLARGYRDLNANQKTFCEDAYGREQVFRDGGRNRNRM
                     TPDSVARLFKEIALGEIVGPSGTQEMLALLARDVTPDKPYDDLELEDARLAGRRLPAG
                     TRLWSKSGDAYDYHHLVARAALPSGADFVIAVFTKGVRTVPDVIPRIYERVGAHFAAA
                     AASASAAR"
BASE COUNT          367 a          884 c          838 g          369 t
ORIGIN      
        1 ctccttctcg acaacgtccc gcacgtcaag gcgtactggg ttcagatcgg gacgaagctc
       61 gcgcaggtcg cgctcttctt cggcgcggac gacctcgtcg gtacggtcgt cgacgaaacc
      121 atcacgcacg ccgccggcgc gacaaccgcc tccggcatga cccgcccgga attcgagagg
      181 ttgatccgcg agtccggccg cgagcccttc gagcgggacg ccgagtaccg gcgcgtcgcg
      241 cggagcgacc ggcccgtccc cgcggcgccc ccctcgaccg agaccgtttc cgtccccgct
      301 tgatcccggc caggcgcttg ggcgcgctgt tcctcgcgaa cgttctcgcg tcggcgtgcc
      361 tcgcctccgg ctatcgcacg aagccccgcg tcgcgatcgt ggacggggac ccggtcgtcc
      421 agatggcgga gcccggccag tttcgatcgg cgaccaaccc gcggtgggca gcggcagacg
      481 atcagacgga cgcgccgaaa caccatcagc gcgtcctcgg cctcgagatc cacggggtac
      541 gcatcgccta cccgatcggg ctcctcgacc gcgccgaggt cgtcaacgac ggtctcgacg
      601 gggtcgcatg ggtcgcggcg cggtgcgcgt tgacgcacgt cgcagcggtg tacgaccgcg
      661 tcgtcgatgg ccgaaccctc accttcgaga actcgggagc gctctggcgc gacacgctcg
      721 tgctccgcga ccgcgagaca gggacctact ggacggccgc gacgggcgtc gcgctttcgg
      781 ggcctctcgc cggccgccgg ctgcgctcga tccccgcggc gtacacgacg gcgctcgcct
      841 ggcaacgcgc ctttcccgac tcccgctggg cggacctcgg gcttccgaca tcggtaccgc
      901 tgtcgatgaa gatctacggg gcttctccgt ggcagggggt ctcccgagag aagactcgcg
      961 accgccgaca cccggccaag aaggagtttc tcgaggtcgc catcggccgc gaagcgctcg
     1021 cgttcacgcg gggcgagatc cgccgccggc gaaccgccgt cgcgaccgtc ggcggcgaga
     1081 cgattcggtt ggagtgggac gccgcgaccc aggccgccca ggcctgggcc ggtgaaggcg
     1141 cgggacgccg cgagctcgcg gtcgttccga tgtactggtt cgcgctcgac cgccacttcg
     1201 atgcgatccg gtccctcgcg gtcggggaat cggagcgcgg tcaatcgaga ccgcggttcc
     1261 gccgccgtcg gatcagcgat cggccggcag cgtccagatc tccggcggcg tgatcccggc
     1321 ggcccggtcc gcttcgcgcg tcgcttcgtc gagagcggct ccgatccgct cctgcgcctc
     1381 ctcgtccgag tctcgctcgt cgatcggaaa gggctctccg taaaggacga cgttccgcga
     1441 gaacggcatc ggaaggagat accggtccca ggacgacagg aatctcgggc gagagctcga
     1501 cgaggtgacc gggagaatcc agcccccggt cagtcgggcg agctggacga tcccgggctg
     1561 cacctggcgc ggcgggcccg tgggcccgtc gaccgtcagc gcggcattct cgccgccgcg
     1621 gatccggcgg accatctctc gaagcgcctc ggatcccccg cgggtggagg agccccgcac
     1681 cacgaagtag ccattcctct cgagccatcc ggcgatgatc tcgccgtcct tcgagcgcga
     1741 cgccatcgtc acgatccctt cgtgccggtg cgcctggatc gagaggaaca tccgcccgtg
     1801 ccacagcgtg aagaggatgg ggacgccccg cgccttcagc ccgagatacc gctcgaagtg
     1861 catcctctcg acgcggagcg tggagcggtg caggtacaag agagcccccg cgagacggga
     1921 gagaatccgc gatcccacgg cggaccgttc taccgcgcgg ccgatgccga cgccgccgcc
     1981 gccgcgaagt gtgcgccgac cctctcgtag atccgcggga tcacgtcggg cacggtcctc
     2041 acgcccttcg tgaagacggc gatcacgaaa tcggcgccgc ttggaagtgc ggcgcgcgcc
     2101 acgaggtggt ggtagtcgta ggcatccccc gacttggacc aaagccgcgt ccccgccgga
     2161 agcctcctgc cggccaggcg tgcgtcctcg agctccaggt cgtcgtaagg cttgtcgggc
     2221 gtgacgtcgc gcgcgaggag cgcgagcatc tcctgcgttc cggagggccc gacgatctcg
     2281 ccgagagcga tttccttgaa gagccgcgcg acggagtccg gcgtcatccg gttccggttg
     2341 cgcccgccgt cgcgaaagac ctgctcgcgg ccgtacgcgt cttcgcagaa cgtcttctga
     2401 ttcgcgttca agtcgcgata gccgcgcgcg agaaagaagc ggttcacggc gtttcgct
//
LOCUS       DASWAV010000344         5735 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U2665
            k141_619047, whole genome shotgun sequence.
ACCESSION   DASWAV010000344 DASWAV010000000
VERSION     DASWAV010000344.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960316
            Sequence Read Archive: SRR10260007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 5735)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5735)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:47:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,929
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5735
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_619047"
                     /isolate="SMAG_U2665"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.36444 N 132.31889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            322..1272
                     /locus_tag="VF958_07115"
     CDS             322..1272
                     /locus_tag="VF958_07115"
                     /inference="COORDINATES: protein motif:HMM:NF025006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX9148916.1"
                     /translation="MRKGTRAVTALAAFGLATLLFAGAQGRLKGKVTDSSGAPIEGAT
                     VTITTPNLRTFKMSLKSDKRGDWGTILNDATMPYHVRIEKDGFAPAESDKKVPVGDVG
                     VVDAKLLTTAEAGAAASGHGAPAPPSPSDLAAISFNEGVDLLSAGNKPGAEAKFLEAV
                     GKNPDLPQGWQALATLAYEKKDWAKTIEYGQKAIDLDPSLTQLYGVMATAASQSGDKK
                     AAAEWQAKFEEANPESAESMYNKGIDAYNKKKVKDAEAMLLKAVEAKPDFALAHFWLG
                     MASFNLNKKAAAKEHLEKYLELEPSGSEASTAKELLPLLK"
     gene            1642..2664
                     /locus_tag="VF958_07120"
     CDS             1642..2664
                     /locus_tag="VF958_07120"
                     /inference="COORDINATES: protein motif:HMM:NF024676.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WYL domain-containing protein"
                     /protein_id="HEX9148917.1"
                     /translation="MGRKGAFKDRPLWRRLQTIHHEIKESRHPNATKLAAALDVSTKT
                     VQRDLDYLRNELAAPIEFDRDENGYFYSRADYVLPFLPVDGRDLFSIGVAAQVLSLFG
                     GTPLGRDLKSCYERLAKLMPPAVRLRPEIVMEKLALRAAAFRPVREETWQAVSESLQR
                     GVALSIRYHRPGEPPGDSRTVKPYSLVVFGRDWMVLALDETDGIVKNFYLARMEDAHP
                     TSQRYTIPRDFDPDAFFRNTFGIFVGGSKPFRFRVRFSKEISEEVRELKFHPKQKLER
                     GAEGEAILELPAESLKEARRFILGYGKDAVALSPPELVEELRKETAYLREEYEKSSKS
                     RVRNPK"
     gene            2624..3304
                     /locus_tag="VF958_07125"
     CDS             2624..3304
                     /locus_tag="VF958_07125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9148918.1"
                     /translation="MRRVRSPGFGIQSSLSVLLVLFATACGSATEGLAPLAYPDPSGI
                     CPAGRTGWKLEVLDRRAERPNSENVVALIGESVRRSFPGCRWDGEGDAGAGVIRVEVH
                     RFGTTAAENTWDAAAEWTVTASDPAGATLTEFEADEEVSRPNYRGSNNARESLKEVFD
                     RALRRTLAGLRVVSSARSGRPPGGTPEAADRPVAWETRRTTHSHLETSVDRTSSRESA
                     SVERFPQS"
     gene            3337..3879
                     /locus_tag="VF958_07130"
     CDS             3337..3879
                     /locus_tag="VF958_07130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9148919.1"
                     /translation="MAIFAAALLAAALPLVAATGSGANQPYELSSMFSGTWQGSTPGN
                     ELRLQIRSATTDPSHLYDLYVQVSGKYQGQNTRQQGLIRLEGQGKGVYVGYIPHFDPT
                     VTSMSTDVARFTDEEANAACGLVMEPKGDGFAAETVGSTCARAIRGALGKWTIEVEPG
                     SIRIRSVSSGETLRFKKSGK"
     gene            complement(3934..4788)
                     /locus_tag="VF958_07135"
     CDS             complement(3934..4788)
                     /locus_tag="VF958_07135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007366119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HEX9148920.1"
                     /translation="MLENRLPLDRPLVVFDTETTGTNPRLDRIVEIACVKVHPDGRRE
                     EWVRRFNPGIRIPAASTVIHRIRDADVESMPRFADCAAELAAFLEGCDLAGYNMTGFD
                     LPVLRNEFYRAGIVFDVTSRRLVDGQRIFFSREPRNLSAAARFYCETDHDGAHGALAD
                     ARMTLRVLAGQLDRYADLPRTVAELHELFCAGIDQDMDPEGRIRLINGEPTVNFGRHR
                     GRTLRALSREEPGVLRWILKGDFSRFVKEIVAKFMPKEETEKAMAASLPSAKPEPQPT
                     MGSLFPPE"
     gene            4894..5211
                     /locus_tag="VF958_07140"
     CDS             4894..5211
                     /locus_tag="VF958_07140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9148921.1"
                     /translation="MTVFKIENFWRRGSRGAVRCTARLSRRLSADESQRIERLANTFV
                     ERSVIVHECGPDEVEEWHTKLVDALAGAATAAPRRVAVGERRSRRSQQRAPNALRQLD
                     PPA"
     gene            complement(5240..>5735)
                     /locus_tag="VF958_07145"
     CDS             complement(5240..>5735)
                     /locus_tag="VF958_07145"
                     /inference="COORDINATES: protein motif:HMM:NF024491.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phospholipase D-like domain-containing protein"
                     /protein_id="HEX9148922.1"
                     /translation="LLYYMAIQAARKTIWMENAYFVPDGQIRRGLVSAAARGVDVRVV
                     VPGPHIDIPFVRMASRHHYGELLDGGVKIYEYQPTMMHSKVMVVDSVWTTIGSINIVN
                     RSMKKNAEANVAIYDRGFAGIVEKSILADLEKSQVFTWARWKKRGFLARLGETFSWLF
                     SENY"
BASE COUNT         1032 a         1840 c         1900 g          963 t
ORIGIN      
        1 cagccgctcg aggcgcttgc cgggattggc cccagcctcg cccgggccat tcgcgacgtc
       61 gctcgcgggc tccacgtgat gccgatggga aagtattccc ttctcgccga cattctcgac
      121 gtgacctcgg ctgctggcca ggcgagccgc gcgtaagtcg agacgaaagt cggaccggaa
      181 tacctccgcc cgcgagatcg tttttgtcgc cggcctggga tcgcgctcgg cacggtccgt
      241 ctccgtgccc ctctccgatt ccatagaatt cggaaagatt cccggcggat ggagttatcg
      301 cctcgccggc tggagggaaa gatgagaaaa ggaactcgcg ccgttaccgc gctcgccgct
      361 ttcggcctgg cgacgctcct cttcgcagga gcacaggggc gtctgaaagg aaaggtcaca
      421 gactcctcgg gcgcacccat cgagggcgcg acggtgacga tcaccacccc gaacctgcgg
      481 acgttcaaga tgagtttgaa gtccgacaag cggggggact ggggaacgat cctgaacgac
      541 gcgacgatgc cgtaccacgt gcggatcgag aaggatgggt tcgcgcccgc cgagtcggac
      601 aagaaagtgc ccgtcggcga cgtcggcgtg gtcgacgcga agctcctgac gaccgccgag
      661 gccggcgccg cggcgagcgg ccacggtgct cccgcgccgc cctccccgag cgatctcgcg
      721 gcgatcagct tcaacgaagg ggtcgacctt ctctccgcgg gcaacaagcc gggcgccgag
      781 gcgaagttcc tcgaggcggt cgggaagaac ccggacctgc cgcagggatg gcaggctctc
      841 gcgactctcg cgtacgagaa gaaggactgg gcgaagacga tcgagtacgg gcagaaggcg
      901 atcgatctcg acccgtcgct cactcagctc tacggcgtca tggccaccgc ggcctcgcaa
      961 tctggagaca agaaagcggc ggcggagtgg caggcgaagt tcgaggaggc gaatcccgag
     1021 tcggcggagt cgatgtacaa caaggggatc gacgcctaca acaagaagaa agtcaaggac
     1081 gcggaagcga tgctgctgaa ggcggtcgag gcgaagccgg acttcgcgct cgctcacttc
     1141 tggctcggga tggcatcgtt caacctgaat aagaaggcgg ccgcgaagga gcacctcgag
     1201 aaatatctgg agctcgagcc gagcggatcg gaagcgtcta cggccaagga gctgctgccg
     1261 ctgctgaagt aggacctgcc ggcggaaccc ggaacggcga gctttgcgag ctcgccgttt
     1321 tgtttttcag acggaaacgc gatctcgaac ccggcgcggg cggtcggacc gagccggttg
     1381 cggcgaagcg gtcggcggat tcagaagccg gtgcacccag cggagcagcg catcgaatat
     1441 gtcggggcgc ctctcgtctc gaaaccgaac gtcgtgcggt ctcatagctg cgcttgagca
     1501 gcaagcccgc agccaagctg ccaccttcaa cctaactctt ttctcgcgac agacttgacc
     1561 agggaagcct acggcctctg ctgaccgtga gcggcgcctc tcgccccgcg ccgagcgaca
     1621 ttttctgcca caattcccgg cgtgggccga aagggcgcct tcaaggatcg acctctctgg
     1681 cgccggctcc agacgatcca ccacgagatc aaggaaagcc gtcatccgaa cgcgacgaag
     1741 ctcgccgccg cgctcgacgt ctcgaccaag acagtccagc gggatctcga ctacctgcgc
     1801 aacgagctcg ccgcgccgat cgagttcgac cgcgatgaga acggctactt ctactcgcgc
     1861 gcggactacg ttctgccgtt tctcccggtg gacggacgtg atctcttttc gatcggtgtc
     1921 gccgcgcagg tgctgtcgct cttcgggggc acgccgctcg gccgggacct gaagagctgc
     1981 tacgagcgcc tggcgaagct gatgcccccg gcggtccggc tccggcccga gatcgtgatg
     2041 gagaagctcg cgttgcgcgc cgccgccttc cgcccggtcc gggaagagac gtggcaggca
     2101 gtctcggagt cgctgcagcg cggtgtcgcg ctttcgatcc ggtaccaccg gcccggagaa
     2161 cctcccggcg attcgcgaac cgtcaagccg tattcgctcg tcgtgttcgg gcgggactgg
     2221 atggtcctcg cgctcgacga gaccgatgga atcgtgaaga acttctatct cgcacggatg
     2281 gaggacgcac acccgacgtc gcagcgctac acgatcccgc gcgatttcga tccagacgcc
     2341 ttcttccgga acactttcgg gatcttcgtg ggcggatcga aaccgttccg attccgcgtg
     2401 cggttctcga aggagatctc cgaggaggtt cgcgagttga agttccaccc gaagcagaag
     2461 ctcgagcgcg gtgcggaggg cgaagcgatt ctcgagcttc ccgcggaatc gctcaaggaa
     2521 gcccggcggt tcattctcgg ttacggaaag gacgcggtgg cgctttcgcc gccggagctc
     2581 gtcgaggagc tccgaaagga gacggcatac ttgcgtgagg aatatgagaa gagttcgaag
     2641 tccagggttc ggaatccaaa gtagtctctc ggtcctgctg gtcctgttcg ccacggcgtg
     2701 cggatcggcg accgaagggc tcgcgcccct cgcctatccg gatccgtcgg gcatctgtcc
     2761 cgccggaaga acaggatgga agctcgaggt gctcgatcga cgcgccgagc ggccaaactc
     2821 cgaaaatgtc gtggcgttga tcggcgagtc ggtgcgccgc tcctttccgg ggtgccgctg
     2881 ggacggcgag ggggatgccg gcgccggggt tatccgagtc gaggttcacc gcttcggcac
     2941 gaccgccgcc gagaacacct gggacgcggc ggcggagtgg acggtcacgg cttccgatcc
     3001 cgccggggcg accttgaccg agttcgaagc cgacgaagag gtctcccgtc cgaactatcg
     3061 gggctccaac aatgccaggg aatcgctgaa ggaggtcttc gaccgggcgc ttcgccggac
     3121 cctcgccggc cttcgggtcg tctcctcagc gaggtcaggt cgtcccccgg gggggacgcc
     3181 ggaggcagcg gatcgcccgg tggcatggga aaccagacgc acaactcact cacatctcga
     3241 aacttcggtc gatcgcacgt cgtcgcggga gagcgcttcc gtggaacgct tcccgcaatc
     3301 ttgaccggcg gaggtcttat gaagcggaca tctcgagtgg cgatttttgc agccgccctc
     3361 ctggcggcag ctcttccgct ggtcgcggcg acggggagcg gcgcgaacca gccgtacgag
     3421 ctttcatcca tgttttcggg cacctggcag ggctcgacgc caggcaacga gctgcgactc
     3481 cagatccggt cggcaacgac cgatccgagt catctctacg atctctacgt ccaggtgtcg
     3541 ggcaagtacc agggccagaa cacgcggcag cagggcctga tccgactcga gggacaggga
     3601 aagggcgtgt atgtcggata catcccccac ttcgatccca cggttacctc gatgtcgacg
     3661 gacgtggcgc ggttcaccga cgaggaggcg aacgccgctt gcgggctcgt catggagccc
     3721 aagggcgatg gcttcgcggc ggagactgtg ggctcaacgt gcgcccgcgc gattcgtggc
     3781 gccctcggca agtggaccat cgaggtcgag cccggatcga tccggatccg cagcgtcagc
     3841 tccggcgaga cgctcaggtt caagaagagc gggaagtaag ccccctcacc ccgaccctct
     3901 ccccgcgagg cggggagagg gagaccttga gggctactcc gggggaaaga gactccccat
     3961 cgtcggttgc ggttcgggct tcgccgacgg tagggacgcc gccatcgcct tttcggtctc
     4021 ttccttcggc atgaacttcg cgacgatttc cttcacgaat ctcgagaagt ctcccttcag
     4081 gatccagcga aggactcccg gctcctcgcg cgagagagcc cggagcgtcc gcccgcgatg
     4141 tcgtccgaag ttgaccgtgg gctcgccgtt gatcaggcga atgcgaccct ccggatccat
     4201 gtcctgatcg atcccggcgc agaaaagctc gtgcagttcg gccaccgtgc ggggcaaatc
     4261 cgcgtagcgg tcgagctggc ccgcgaggac ccgaagcgtc attcgcgcgt cggcgagggc
     4321 gccgtgcgcg ccgtcgtggt ccgtctcaca gtagaagcgc gcggcggccg agagatttcg
     4381 cggttcccgc gaaaagaaga tccgctgtcc gtcgacaagc cggcggctcg tgacgtcgaa
     4441 gacgattccc gcccggtaaa actcgttccg gagaacgggg agatcgaaac cggtcatgtt
     4501 gtatcccgcg agatcgcagc cttcgaggaa cgcggcgagc tcggcggcgc agtcagcgaa
     4561 ccgcggcatg ctctcgacgt cggcgtcgcg gattcgatga atcaccgtcg acgcggccgg
     4621 aatccggatt cccggattga accgccgcac ccactcctcc cgccgaccat cgggatggac
     4681 tttcacgcag gcgatctcga ctattcggtc gagccgcggg ttcgttccgg tcgtctccgt
     4741 gtcgaaaacg acgagcggac ggtccagggg gaggcggttc tcgagcaccg ttccgcatgt
     4801 taccagcggg attccggggg aggcgctctc ccgcggcgcg atggcctgcc tcttgctcaa
     4861 ccttcaatcg tgctgattcc gatgttccct cgagtgaccg tgttcaagat cgagaacttc
     4921 tggagacgcg ggtcccgcgg cgccgtgcgg tgcactgccc gactcagccg gaggctgagc
     4981 gcggatgagt ctcagcggat cgaacggctc gcgaacacgt tcgtcgagcg ctcggtaatc
     5041 gttcacgagt gcggcccgga cgaggtcgag gagtggcaca cgaagctggt ggatgctctc
     5101 gcgggcgccg cgaccgccgc accgcggcgg gttgccgtcg gagagcgccg ctcgcgacgc
     5161 agccagcaga gggccccgaa cgcccttcgg cagctcgacc cccccgccta gccgggtcgg
     5221 cgcaaggagt gcgggagggt cagtaattct ccgagaagag ccaggagaac gtctctccca
     5281 gccgcgcgag gaaaccgcgc ttcttccacc tcgcccacgt gaagacctgg ctcttctcga
     5341 gatcggccag gatcgacttt tcgacgatgc cggcgaaccc gcggtcgtag atcgcgacgt
     5401 tcgcctccgc gttcttcttc atcgagcggt tgacgatgtt gatggacccg atcgtcgtcc
     5461 acacactgtc caccaccatc accttgctgt gcatcatcgt cggctggtac tcgtagatct
     5521 tcacgccccc gtcgagaagc tcgccgtagt ggtggcgcga cgccatccgc acgaacggga
     5581 tgtcgatgtg gggtccgggt accacgacgc gcacatcgac tccgcgcgcg gcggcgctga
     5641 cgaggccgcg acggatctgg ccgtccggga cgaagtaggc gttctccatc cagatggtct
     5701 ttcgagccgc ctggatcgcc atgtaataca gcagc
//
LOCUS       DASWAV010000345        10510 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U2665
            k141_57420, whole genome shotgun sequence.
ACCESSION   DASWAV010000345 DASWAV010000000
VERSION     DASWAV010000345.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960316
            Sequence Read Archive: SRR10260007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 10510)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10510)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:47:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,929
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10510
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_57420"
                     /isolate="SMAG_U2665"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.36444 N 132.31889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..587)
                     /locus_tag="VF958_07150"
     CDS             complement(<1..587)
                     /locus_tag="VF958_07150"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: protein motif:HMM:NF013413.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070573 - metallodipeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane dipeptidase"
                     /protein_id="HEX9148923.1"
                     /translation="MKKALLLPAVLLLIAASPPERGDDLHARAMRLQRSAIIVDTHED
                     VPEQLEKEWVDIGVRQKTGHVDIPRWREGGVTAPFLAAYVPSAYAKSGKSAKKALEFI
                     DLIHSLVDSHPKDLVFADSIAGIRAAKKQGKIAVLIGIEGGHAIEDSLGALSAFYRLG
                     ARYMTLTHTNTNDWADSAGSFFSFDFNPTRTAVHNG"
     gene            complement(697..1806)
                     /gene="tgt"
                     /locus_tag="VF958_07155"
     CDS             complement(697..1806)
                     /gene="tgt"
                     /locus_tag="VF958_07155"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008479 - tRNA-guanosine(34) queuine
                     transglycosylase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HEX9148924.1"
                     /translation="MPPFLIQSRDAASAARLGSLATAHGEVDTPCFLPVGTRGAVKGV
                     EFDRLEEWDCRAILSNTYHLMARPGRDVIRAAGGLHAFLGWRRAILTDSGGFQVMSLS
                     SRRRVTEEGVEFRSPEDGTRHFLTPELAVDLQSEFGVDIAMTLDVCPPFPAERQTVEE
                     ACRLSGLWAVRSRARWRGGERSGLLFGIVQGGTHEDLRRSAASSLGDLELDGYAIGGV
                     AVGESKEAIRAITAFTAPLLPEQKPRYLMGVGTPADLLASVRAGVDLFDCVLPTRNGR
                     MGHAYTRAGEVTIKHERFKTDLAPLDPGCACPVCRRHTRAYLRHLFVLGDFSAPMLLS
                     IHNVFFYLDWMKRIRAAIADGELDRLEAPPEKKID"
     gene            1932..2249
                     /locus_tag="VF958_07160"
     CDS             1932..2249
                     /locus_tag="VF958_07160"
                     /inference="COORDINATES: protein motif:HMM:NF021083.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FYDLN acid domain-containing protein"
                     /protein_id="HEX9148925.1"
                     /translation="MPDLGKKFECYNCGTKFYNLGRPEAICPKCGSNQKNAKSEDAPV
                     AAPRPPRRAAIIMEPPPEENPNEFSEEPAAVEEEESSDDEDADEEIEIVDSGRTGTEE
                     EEY"
     gene            2185..2766
                     /gene="folK"
                     /locus_tag="VF958_07165"
     CDS             2185..2766
                     /gene="folK"
                     /locus_tag="VF958_07165"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR01498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003848 -
                     2-amino-4-hydroxy-6-hydroxymethyldihydropteridine
                     diphosphokinase activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HEX9148926.1"
                     /translation="MPTRKSRSSTAAARGRRKKSTDPSPPGAIAYLGLGSNLGNRRAH
                     LEMALCEIARVAPLRRISSFYRTAPVGFRDQPDFWNAVVEITWRGSPRGLLKAAQVVE
                     RRVGRTPTFVNGPREIDVDVLDFGGRIREKGDPILPHPRLNERRFALAPLAEINPRWK
                     DPRTGRRVGELLAALPGNPRARRIALRPRRPRE"
     gene            complement(2740..3564)
                     /locus_tag="VF958_07170"
     CDS             complement(2740..3564)
                     /locus_tag="VF958_07170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9148927.1"
                     /translation="MTCLVLASAVALTEAESAMAAAPTPVLAAADAAWVRRDEGRAGS
                     RANSRRISEAVSGYEAAAASPDSIEARWKLARALFFEGKFTGLDREAQRAQFERARDA
                     SEEAVTLLQRRKGSLAADPDAAPTYFWAAVAWGEWAQVVPRMKAARAGAATKIRDYAA
                     KVVAIDPKFEEGGGYRILGRLHDRAPRIPFVTGWVSRAEALRNLRLAVEVAPRNFVNR
                     HFLAEALAAGDRSGREEAVRIEQEVVADAPSPSHLVEDVAVQEEAKRHLLAWTAGS"
     gene            complement(3643..4731)
                     /gene="mnmA"
                     /locus_tag="VF958_07175"
     CDS             complement(3643..4731)
                     /gene="mnmA"
                     /locus_tag="VF958_07175"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012097140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HEX9148928.1"
                     /translation="MRVAVAMSGGVDSSVAALLLKESGADVVGLSMQLWDHSSDSGRT
                     GRCCTLDDLADARRVAWALDIPHYVVNLEEEFREDVVRPFVASYLAGETPIPCSACNS
                     KVKFATLWERARAMGCETIATGHYARVEKDAITGRPTLMKGLDPAKDQSYFLYDLTPE
                     QLAAARFPVGALTKAEVRAHARRASLPTADKEESQEICFVPAGSRAGEFVASAAPGFG
                     LPLPSSTGAVENARGERLGGHDGHYRFTVGQRRGIGVAAAERLYVISIDPAANRVVVG
                     SAAEVDCSEARVSDVRFPSGDPPSAPFRADVRVRHRAAEVPAMIFPAPNRTARVVFDR
                     PVRAIAPGQSCVFYRGDVVVGGGVLRRR"
     gene            complement(4741..5901)
                     /locus_tag="VF958_07180"
     CDS             complement(4741..5901)
                     /locus_tag="VF958_07180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013495647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase family protein"
                     /protein_id="HEX9148929.1"
                     /translation="MIYLDNNASTALDPAVREAMTVAADSYGNPSSVHSAGRRARRAV
                     EESRDEVARLIGARSDEIFFTSGGTEANALAILGTASSEETGRIVLTAAEHPSVREPV
                     TSLADRFEIRTVPLEQSGALDPAKVLDTATPATRLVSVMAANNEYGGIYPVAEIAAAL
                     AGRAVPIHTDAVQAAGRLPIDVESWGVDLLSLSAHKLHGPKGVGALYVRRGRPIRALF
                     PGGGQEKKIRGGTENTIGIVGFGVAVRLARERLALEPRGISVLRDRLERGILESIPDV
                     RAVGGGAPRLPNTTAILFGELAGDALLFRLDLEGVAVSVGSACSSGTLEPSPAILALG
                     FPRQQAKGVVRFSLSRLTTEAEIDRVLEILPRIVAEARAIRSRAGVPASAVP"
     gene            5977..10197
                     /locus_tag="VF958_07185"
     CDS             5977..10197
                     /locus_tag="VF958_07185"
                     /inference="COORDINATES: protein motif:HMM:TIGR03436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HEX9148930.1"
                     /translation="MKRAAVLAFLAASFLLPGAGAAGTAPEPSRKDRIAALPEEERKW
                     LTEFVEPIILPDEEKLFLALTEPYQREMFKADFWERRERTGLAAPLGPGYRQRYEHLR
                     DLAGSQYDGLTSDAGRLVVRLGEPNGIEEVGSCDGVLRQVEVWTYRSSVGKPSQHVFY
                     RPTFGAQRKLWSPGLGDAALFEPASCLTSLSELCLPATQPLKTNDPVCSEPGAVSKRC
                     GAEGCRFARVAEDVRGRHGESIAELALLSTPPKVSTEGLDQLRNRLASLPDSKARPIE
                     VAASGVVAMANAQSAPAPPPAPAAPVKELSKKERLAAMPEDDRKWLTEFVAPIIQPEE
                     EALYLQLTEPHQREIFKEAFWARREKANLVPPLGPGFRYRYQEVRELADTKYDGWRND
                     AGRMVIARGEPAEIEAMTSCGDTVRDLEFWKYSNLGGSGRGKAEFLFYRPSPGTPRKL
                     WYVGIPEEQLYTPASRPLTPQEQAIECSNGPKPLSASLPRPNHECICRLFEFMQQAKA
                     LASPASGMMEAGRALTPDKISTEDLSQIAGNFPGIPTKGAKSLGVEGPSSSAPAGATA
                     KPAAQTASSTPAPTHRKLSKKEVKELSARLAQKYRDFLELVDLIITDDEREIFVQIAD
                     DYQKDRFIESFWRRRSIDSQGLRTDYQAVHTHRVEIAKEQFKNLNNDRAKMIIINGPP
                     DAVIPIDCPEVFVPIQIWYYDRLEIMKSKAYLIFYAPYGIGPDYKLWLPLDGEGVLTV
                     GGGFGGAAAGGRPIGGRRIDYDRCPETRTLRQAIAYSTAVLGSGASALAGAGKLFQPP
                     VIETEGVDQILTMTTELATGSAPLGLAKLVRFPEMRANKIGVDLSLLVAKSDLKPRDL
                     GEEKFYNVDVIGEVVKGERLIDNFKYRFDIPTQEVGGEKIPLTVRRYLYPGEYNLVLK
                     VSDGNQSAEGRITDKLTVPEQPDAPPPEVLAKRADASATLEKARDLGLLPSAIMLLPV
                     AKEIVTGLQRFETRAADNVKAVEFYLNGSRMMTKTRPPFAADLNLGPLPRKHTIRVVA
                     FGTTGRAIGEDEYIVNEGREIFRVRILSPEKGAKASGPTKVVAAVVVPEGKALEKMEF
                     YSNERRVATLYQPPFEQTVNISEGKSLGYVRVIGTLEDGTVSEDLRYVNAPAYISEVS
                     VDAVELYTTVTDKGRPVQGLQATNFKVFEDGAIQKIESFEYVKNLPLTLGVLVDTSAS
                     MLESLPEAQAAALSFIEYSIGPKDRAFTVSFENEPYLLTKLTNRKDRLVRSLAGLRAE
                     GSTALYDAIIYGLYQFTGVKGKKALVVLSDGKDTASKFDYETLVEYVKKAGISIYGIG
                     LKISGADLEVKYKLNKLAQQTGGQTFYVDSAKNLESVYRQINDDLRSQYLLTYYSTNS
                     TGKDKWRKVDIKVEPSSMQARTITGYYP"
     gene            complement(10188..>10510)
                     /locus_tag="VF958_07190"
     CDS             complement(10188..>10510)
                     /locus_tag="VF958_07190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9148931.1"
                     /translation="GGHDAVLPTGQSSQHLSCIAVVPRLAEDGPVEQHESVGSEDPVA
                     RMSEGGGGSLRARESRRRGGTRLARQNRFVDVGGTDSKRNSERRQDLGPTGGGGGEDE
                     VCYG"
BASE COUNT         1823 a         3617 c         3479 g         1591 t
ORIGIN      
        1 ccgttgtgga cggcggtcct cgtcgggttg aagtcgaaag agaagaagga ccccgccgag
       61 tccgcccaat cgttcgtatt ggtgtgggtg agcgtcatgt atcgggcccc gaggcggtaa
      121 aacgcggaga gcgcgccgag agagtcctcg atggcgtggc cgccctcgat cccgatgagc
      181 acggcgatct ttccctgctt ctttgccgcg cggatgccgg cgatggaatc ggcgaacacg
      241 aggtctttcg gatgcgagtc gacgagagag tgaatcaggt cgatgaactc gagagccttc
      301 ttcgccgact ttcccgattt cgcgtacgcg gacggaacgt aggcggcgag gaacggcgcc
      361 gtcactcccc cctcccgcca ccgcgggatg tccacgtgcc ccgtcttctg gcggacaccg
      421 atgtccaccc actccttttc gagctgctcg gggacgtcct catgggtgtc gacgatgatc
      481 gcgctccgct ggagccgcat cgcgcgggca tgcaggtcgt cgccccgctc gggcggactc
      541 gccgcgatca ggaggaggac ggccggcagc agcaacgcct ttttcatggc gcgagaggct
      601 aacatccgtc ggcgtcggtt gctcgggacc tgcggaatcg agacagacca ctcattcatg
      661 ttcgtcggcg tcgctcgcgc cgcggaacac gaagagctaa tcgattttct tctccggggg
      721 agcctccagc cgatcgagct ccccgtcggc gatcgcggcg cgaatgcgct tcatccagtc
      781 caggtagaaa aagacgttgt ggatcgacag aagcatcggc gcggagaagt caccgagcac
      841 gaagaggtgg cgcaggtacg cgcgcgtgtg gcggcgacac accgggcagg cgcacccggg
      901 gtcgagcggc gccagatccg tcttgaaacg ctcgtgcttg atcgtcacct cgccggctcg
      961 cgtgtacgcg tgccccatgc gcccgtttct cgtcggcagc acgcagtcga agaggtccac
     1021 gccggcgcgg accgacgcga gaaggtccgc gggcgtgccg acgcccatca ggtagcgcgg
     1081 cttctgctcc ggcaggaggg gcgcggtaaa ggccgtgatc gcccggatcg cctctttgga
     1141 ctcccccacg gccacgcctc cgatcgcgta gccgtcgagt tcgaggtcgc cgagcgagga
     1201 agccgcgctc cgcctcaggt cctcgtgcgt tccgccctgc acgatcccga agaggagccc
     1261 gctccgctcc cccccgcgcc atctcgcccg tgaccggacg gcccagagtc cggagagccg
     1321 gcacgcctct tccactgtct gccgttcggc cgggaacggc gggcagacgt cgagcgtcat
     1381 cgcgatgtcg accccgaact ccgactggag atcgacggcg agctcggggg tcaggaagtg
     1441 ccgggttccg tcctccgggc tgcggaactc gaccccctct tccgtcaccc ggcgtcgtga
     1501 cgagaggctc atgacctgga atccccccga gtcggtcagg atcgcgcgcc gccagccgag
     1561 aaacgcgtgc agcccgccgg cggcccggat cacgtcgcgg cccgggcggg ccatcaggtg
     1621 atacgtgttc gagaggatcg cgcggcagtc ccactcctcg agtcggtcga actcgacgcc
     1681 cttgaccgcg ccgcgcgtcc ccactggaag gaagcacggt gtgtccactt ccccgtgagc
     1741 ggtcgcgagc gacccgagtc gggcggccga cgccgcgtcg cgggattgga tcaggaaagg
     1801 gggcatgggc gagattctgc cgggattcgg gagaatcgca acacgcgaat cgctccgtga
     1861 aatcaatgaa atgcggcggt tgccctttgg ggggtcgcgt gctaggtttc gggcccgtca
     1921 gcgggagagt gatgccggac ctcggaaaaa aattcgagtg ctacaactgc gggacgaagt
     1981 tctacaacct cgggcgtccc gaggcgatct gcccgaaatg cggttcgaac cagaagaacg
     2041 ccaagtccga agacgcgccg gtcgccgccc cgcgcccgcc gcgacgcgcg gcgatcatca
     2101 tggagccgcc gcccgaggag aatccgaacg agttcagtga agaacccgcc gcagtcgaag
     2161 aggaagagag cagcgacgac gaagatgccg acgaggaaat cgagatcgtc gacagcggcc
     2221 gcacggggac ggaggaagaa gagtactgac ccttcgcctc ccggggcgat cgcctacctc
     2281 ggcctcggtt cgaatctcgg aaaccggcgc gcccacctcg aaatggccct ctgcgaaatc
     2341 gcgcgcgtcg cgccgctgcg caggatctcg tcgttctatc ggaccgcgcc ggtgggcttt
     2401 cgggatcaac ccgatttctg gaacgccgtc gtcgagatta cctggcgcgg ctcgccccgc
     2461 ggactcctga aggcggcgca ggtcgtcgag cggcgcgtcg ggcgcacgcc caccttcgtc
     2521 aacgggccgc gcgagatcga cgtcgacgtc ctcgacttcg gcggccgtat tcgcgagaag
     2581 ggagatccca tccttcccca cccacgcctg aacgagcgcc gcttcgcgct cgcgccgctc
     2641 gcggagatca acccgcgatg gaaagatccg cgcacgggcc ggcgcgtcgg cgagctgctc
     2701 gcggcgctgc cggggaaccc gcgcgcgcga aggatcgctc tacgaccccg ccgtccacgc
     2761 gagtaggtgt ctcttcgcct cttcctggac ggcgacgtcc tccacgaggt ggctcgggga
     2821 cggcgcgtcg gcgacgacct cctgctcgat ccggactgct tcttctctcc ccgagcgatc
     2881 gccggcggcg agcgcctccg cgaggaagtg gcggttgacg aagttgcgcg gcgcgacctc
     2941 gacggccagg cgcaggttgc gcaaggcctc ggcccgcgac acccagcccg tcacgaacgg
     3001 gattctcgga gcgcggtcgt ggagccggcc caggatccgg tatccgcctc cctcttcgaa
     3061 cttcgggtcg atcgcgacga ctttcgcggc gtagtcgcgg atcttcgtcg cggcgccggc
     3121 tctcgcagcc ttcattctgg ggacgacctg cgcccactct ccccatgcga ccgccgccca
     3181 gaagtaagtc ggcgccgcgt cgggatccgc ggcgagcgac cccttgcgcc gctgaagaag
     3241 cgtgaccgct tcttcgctgg cgtcccgcgc tcgttcgaac tgcgcgcgct gcgcctcacg
     3301 gtcgagcccc gtgaacttcc cttcgaagaa gagcgcgcgg gcgagcttcc agcgtgcctc
     3361 gatcgagtcg ggagaggccg ccgcagcctc atacccggag acggcttcgg agattcggcg
     3421 cgagttcgcc cgtgagccgg cacgcccctc gtcccgtcgg acccacgcgg cgtccgccgc
     3481 cgcgaggacg ggcgtcggcg ccgccgccat cgcgctctcc gcctcggtca gcgcgaccgc
     3541 cgaagcgagg acaagacagg tcaccaccac gtttcgcact ttcccctact cgggatgcga
     3601 aaccgccggt cctaatccaa aattgcggga aaccggccgg ctctagcggc gccggagaac
     3661 gcccccgccc acgacgacgt ccccgcgata gaagacgcac gactggccgg gtgcgattgc
     3721 gcgaacgggc cggtcgaaga cgacccgcgc ggttcggttc ggagcgggaa agatcatcgc
     3781 ggggacctca gcggcacggt gccgcacccg gacgtccgcc cggaacggag ccgacggcgg
     3841 gtctcccgac ggaaagcgca cgtcggagac tcgggcctcc gagcaatcga cctcggcagc
     3901 cgagccgacg acgacccggt tcgcggcggg atcgatcgag atcacgtaaa ggcgctcggc
     3961 ggccgcgacg ccaattccgc ggcgctgtcc gactgtgaat cggtagtgcc catcgtgccc
     4021 gccgagccgc tcccctctcg cgttctcgac ggcgccggtc gaactcggta gcggcaggcc
     4081 aaacccgggc gcggccgagg cgacgaactc ccccgccctc gagccggccg gcacgaagca
     4141 gatctcctgg ctctcctcct tgtcggcagt cggaaggctc gcccggcgcg cgtgagcgcg
     4201 gacctccgcc ttcgtcagcg cgccgaccgg aaaccgggcg gcggccagtt gttcgggggt
     4261 cagatcgtaa agaaagtagg actggtcctt cgcgggatcg agacccttca tgagcgtcgg
     4321 acgtcccgtg atcgcgtcct tttcgacgcg cgcgtagtgg cccgtcgcga tcgtctcgca
     4381 ccccatcgcc cgcgcccgct cccagagcgt cgcgaacttc accttcgagt tgcacgccga
     4441 gcacggaatc ggggtctcgc ccgcgaggta cgacgcgacg aacgggcgca cgacgtcctc
     4501 gcggaattct tcttcgaggt tgacgacgta gtgcgggatg tcgagagccc acgcgacgcg
     4561 acgcgcatcg gcgaggtcgt cgagagtgca gcagcggccc gttcggccgg aatcggacga
     4621 gtgatcccac agctgcatcg agaggccgac gacatcggcg cccgactcct tcagaaggag
     4681 cgcggcgacc gaggagtcga cccctccgga catggcgacg gcgacgcgca tcggccgatt
     4741 ctacgggacc gcggacgcgg gcaccccggc tcgagagcgg atcgctcgcg cctcggcgac
     4801 gatgcgggga aggatctcga gaacgcggtc gatctcggct tcggtcgtga gccgcgagag
     4861 cgagaagcgg accacaccct tcgcctgctg ccgcggaaac ccgagggcca gtatcgccgg
     4921 cgagggctcg agagtcccgc tcgagcacgc gctccccacg gaaacggcga caccctcgag
     4981 gtcgaggcgg aagaggagcg cgtcgcccgc gagttccccg aacaggatcg ccgtcgtatt
     5041 cggcagccgc ggcgccccgc cgccgactgc ccgcacgtcc ggaatcgatt cgaggatccc
     5101 gcgctcgagc cggtcgcgca gcacagagat tccccgcggc tcgagcgcaa gcctttccct
     5161 cgccagtcgc accgcgacac cgaaaccgac gatcccgatc gtgttctcgg ttccgccgcg
     5221 gatcttcttc tcctggccgc cgccgggaaa cagcgcccgg atcggccggc cccgccggac
     5281 gtagagcgcg ccgaccccct ttgggccgtg gagcttgtgc gccgaaagcg acagcaggtc
     5341 gacgccccat gattcgacgt cgatcggaag gcggcccgct gcctgcaccg cgtccgtgtg
     5401 gatcggtacg gcgcgtccgg cgagggccgc cgcgatctcc gccaccggat agatgccgcc
     5461 gtactcgttg tttgccgcca tgaccgagac gagccgggtc gcgggcgtcg ccgtgtcgag
     5521 gaccttcgcg ggatcgaggg cgccgctctg ctcgagcggc accgttcgga tctcgaaccg
     5581 gtcggccaga gacgtgaccg gctcgcgcac cgaagggtgc tcggccgccg tgagaacgat
     5641 acggcccgtc tcttccgacg aggccgtccc gaggatggcg agggcgttgg cctcggtccc
     5701 gccggacgtg aagaagatct cgtccgaccg ggcgccgatc aatcgcgcga cttcatcccg
     5761 ggactcctcg acggcgcgcc gcgcccgacg tccggcggaa tggacggaag acggatttcc
     5821 gtacgaatcc gcggcgaccg tcatcgcttc gcggaccgca ggatccaacg cagtggacgc
     5881 gttgttgtcg aggtagatca tctttttccg ttcgcgatcg taaaactttc gttaaggaat
     5941 tggtgtgtga atccgagggt tagaatcggc acgcggatga agagagccgc agtcctcgcg
     6001 tttctggctg cctctttcct gctccccggg gccggcgcag ccgggactgc ccccgagccc
     6061 tcccgcaaag accggatcgc cgcgcttccc gaagaggagc gcaagtggct gacggagttc
     6121 gtcgagccga tcatccttcc cgacgaagag aagctctttc tcgcgctcac ggaaccgtac
     6181 cagcgggaga tgttcaaagc ggacttctgg gaacgtcgcg agaggacggg cctcgccgca
     6241 ccgctcggcc ccggctatcg ccagcgctac gagcaccttc gggacctcgc cgggtcccag
     6301 tacgacggct tgacgagcga tgccggacga ctggtcgtgc gcctcgggga gccgaacggg
     6361 atcgaggagg ttggaagctg cgacggcgtg ctccggcagg tcgaggtgtg gacgtaccgg
     6421 agttcggtcg gaaagccgtc gcagcacgtc ttctaccgcc ccaccttcgg cgcgcagcgc
     6481 aagctgtgga gtccgggcct tggagacgcg gcgcttttcg agcctgcctc ctgcctgacc
     6541 agcctctccg agctctgtct tccggcgaca cagccgctga aaacgaacga tcccgtctgc
     6601 tccgagccgg gcgccgtgtc gaagcgctgc ggcgcggaag gatgccgctt cgcacgtgtc
     6661 gccgaggacg tgcgcggccg gcatggggag tccatcgccg agctcgccct cctctccact
     6721 ccgccgaaag tttcgaccga gggtctcgat cagctgcgta atcgcctcgc gagcctgccg
     6781 gactcgaaag ccagaccgat cgaagttgcc gcgagtggcg tcgttgccat ggcgaatgcc
     6841 cagtccgctc cggcgccgcc gcccgcaccc gccgctcccg tcaaggagct gtcgaagaag
     6901 gagcgcctcg ccgcgatgcc ggaggacgac cggaagtggc tgaccgagtt cgtcgcgccg
     6961 atcatccagc cggaagagga agccctctat ctccagctca ccgagccgca ccagcgcgag
     7021 atcttcaagg aagcgttctg ggcacggcgg gaaaaggcga acctcgtgcc gccgctcggt
     7081 ccgggctttc gataccgata ccaggaggta cgcgagctcg ccgacacgaa gtacgacggg
     7141 tggagaaacg acgcggggcg gatggtgatc gctcgcggcg agccggctga gatcgaggca
     7201 atgacctcct gcggcgacac ggtccgcgac ctcgagttct ggaagtattc gaacctcggg
     7261 gggagcggcc gcggtaaggc cgagttcctg ttctaccggc cgtcgcctgg aacgccccgc
     7321 aagctctggt acgtcggaat ccctgaggaa cagctctaca cgcccgcgag ccggccgctg
     7381 accccgcagg agcaggccat cgagtgctcg aacggcccga agccgttgag cgccagcctc
     7441 ccgcggccga accacgaatg catctgccgg ctcttcgagt tcatgcagca ggcgaaggcg
     7501 ctggcgagcc cggcgagcgg catgatggag gccgggcgcg ccctcacccc cgacaagatc
     7561 tcgaccgaag acctttcgca gatcgccgga aacttcccgg gcattcccac caagggcgcg
     7621 aagtccctcg gcgtcgaggg cccgtcgtcg agcgcaccgg cgggggcgac agcgaagccc
     7681 gcggcgcaga cggcctcgag cactcccgct cccacgcacc gcaagctctc gaaaaaagag
     7741 gtcaaggagc tctccgcgcg cctggctcag aagtaccggg acttcctcga actcgtcgac
     7801 ctcatcatca ccgacgacga gcgcgagatc ttcgtccaga ttgccgacga ctaccagaag
     7861 gaccgattca tcgagagctt ctggcggcgt cgctcgatcg attcgcaggg gctgcggacg
     7921 gactaccagg cggtccacac gcaccgggtc gagatcgcga aggagcagtt caagaacctg
     7981 aacaacgacc gcgccaagat gatcatcatc aacggcccgc ccgacgcggt catcccgatc
     8041 gactgccccg aggtcttcgt cccgatccag atctggtact acgaccggct cgagatcatg
     8101 aagagcaagg cctacctgat cttctacgcg ccctacggca tcggccccga ctacaagctg
     8161 tggctgccgc tcgacggcga aggcgtcctc acggtcggcg gcgggttcgg gggggccgcg
     8221 gccggaggcc gcccgattgg aggccgccgc atcgactacg accgctgtcc cgagacgcgc
     8281 acgctccggc aggcgatcgc ctattcgact gcggtgctcg gatccggcgc atccgcgctc
     8341 gccggcgcgg gcaagctctt ccaacctccg gtgatcgaaa cggagggcgt ggaccagatc
     8401 ctcacgatga cgacggagct cgcgaccggc tcggccccgc tcgggctcgc gaagctcgtg
     8461 cgcttccccg aaatgcgcgc gaacaagatc ggcgtggacc tgtcgctgct tgtcgcgaag
     8521 agcgacctga agccgcgcga cctcggcgag gagaagttct acaacgtcga cgtgatcggc
     8581 gaggtcgtga aaggcgagcg cctgatcgac aacttcaagt accgattcga tatcccgacg
     8641 caggaggtcg gcggcgagaa gatcccgctg acggtccgcc ggtacctcta tccgggcgag
     8701 tacaacctcg tgctcaaggt ttccgacggg aaccagagcg ccgaagggcg gatcaccgac
     8761 aagctgaccg tcccggagca acccgacgcc ccgccgccgg aggtcctcgc gaaacgggcc
     8821 gacgcttccg cgacgctcga gaaggcgcgc gacctcggcc tgttgccctc ggcgatcatg
     8881 ctccttccgg tcgccaagga aatcgtcacc ggtctccaac gcttcgagac gcgcgccgcg
     8941 gacaacgtca aggcggtgga gttctacctc aacggctcca ggatgatgac gaagacccgc
     9001 ccgccgttcg cggcggactt gaacctcggg ccgctcccgc gcaagcacac gatccgagtc
     9061 gtggcgttcg gcacgaccgg gcgcgcgatc ggcgaggacg aatacatcgt caacgagggg
     9121 cgcgagatct tccgcgtgcg gatcctctca cccgagaagg gcgcgaaagc gtccgggccg
     9181 acgaaggtcg tcgccgcggt cgtcgtcccg gagggcaagg cgctcgagaa gatggagttc
     9241 tactcgaacg agcggcgcgt cgcgacgctc taccagcctc cgttcgaaca gacggtcaac
     9301 atctcggagg gaaagtctct cggctacgtg cgggtcatcg gcacgctcga ggacggcacc
     9361 gtttccgagg acctgcgcta cgtcaacgcg ccggcctaca tctcggaggt gagcgtcgac
     9421 gcggtcgagc tctacacgac cgtcaccgac aagggccgtc cggtccaggg gctgcaggcg
     9481 acgaacttca aggtgttcga ggacggcgcg atccagaaga tcgagtcgtt cgagtacgtc
     9541 aagaacctgc cgctgaccct gggcgtcctc gtggacactt cggcctcgat gctcgagtcg
     9601 ctccccgagg cgcaggcggc ggcgctctcg ttcatcgagt attcgatcgg ccccaaggac
     9661 cgcgccttca cggtctcgtt cgagaacgag ccgtacctcc tgacgaagct caccaaccgc
     9721 aaggaccggc tcgtccgctc gctcgccggc ttgcgcgccg agggctcgac cgcgctctac
     9781 gacgcgatca tctacgggct gtaccagttc acgggcgtca agggaaagaa ggcgctcgtc
     9841 gtcctctccg acggcaagga cacggcgtcc aagttcgact acgagacgct tgtcgagtac
     9901 gtgaagaagg ccggcatctc gatctacggc atcggcctca agatctcggg agccgacctc
     9961 gaagtgaaat acaagctgaa caagctcgct caacagacgg gcggacagac gttttacgtc
    10021 gactcggcga agaacctcga atccgtctat cggcagatca acgacgattt gcgcagccag
    10081 tacctgctca cctattactc gacgaactcg accggcaagg acaagtggcg gaaggtcgat
    10141 atcaaggtcg agccgtcgag catgcaggcg cggacgatca cggggtatta cccgtaacag
    10201 acctcatcct cgcctcctcc tccccccgtc gggccgagat cctgacggcg ctcggaattc
    10261 cgtttcgagt cagtcccgcc gacgtcgacg aatcggttct gccgggcgag acgggtcccg
    10321 ccgcggcgtc gcgactcgcg ggcgcgaagg ctgcctccgc ctccctccga catccgcgcg
    10381 actgggtcct cgctgccgac actctcgtgt tgctcgacgg ggccgtcctc ggcaagccgc
    10441 gggacgacgg cgatgcagga gagatgctgc gaagactgtc cggtcgggag caccgcgtcg
    10501 tgaccgccgt
//
LOCUS       DASWAV010000346         3657 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U2665
            k141_98383, whole genome shotgun sequence.
ACCESSION   DASWAV010000346 DASWAV010000000
VERSION     DASWAV010000346.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960316
            Sequence Read Archive: SRR10260007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 3657)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3657)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:47:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,929
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3657
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_98383"
                     /isolate="SMAG_U2665"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.36444 N 132.31889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1160)
                     /locus_tag="VF958_07195"
     CDS             complement(<1..1160)
                     /locus_tag="VF958_07195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9148932.1"
                     /translation="MPSRRRFAGVLAAAALLPSLFWLGPALLARRAPSFRDQGDFFFP
                     LKLHTADRLLSGRIPLWNPLSGAGEPWLANGQAGVFYPPTAFFLLPWPGAAAILFLLV
                     HFAIAVWGMHRFCKQEGVSDSGALAAAAVFAASGFAASLSAFWNHFGAWAWIPAIAWV
                     ARSGVRTRGALLALALLVGLQAMAGSPELTAMTLLLALVLSLSPRAETDRPWLDRRGR
                     SVVRTAGAVLLGLALAAWVLVPMGELALHSDRRGPLPVAEREPGAIPIPALAAAFARP
                     PGATRTYWLPSLFVGPLALALAAAAFFEKERRSLAMLLLAFGIAGLLIAAAGPPGSWL
                     RAIPPLDRIRYPEKALSATIFAVAALAGLGLDTLRFRAGGRILRVVFPLLAAG"
     gene            1235..3181
                     /locus_tag="VF958_07200"
     CDS             1235..3181
                     /locus_tag="VF958_07200"
                     /inference="COORDINATES: protein motif:HMM:NF014565.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004576 - oligosaccharyl transferase
                     activity [Evidence IEA];
                     GO_process: GO:0006486 - protein glycosylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STT3 domain-containing protein"
                     /protein_id="HEX9148933.1"
                     /translation="MNVASPAFFARHRAFFVAGAVFLGAFLIRTANLRTAFVGGVPQL
                     SPFDELYHAKRIVYSADRPLRVLNFDPSRGTAGAFCPWPPLYDMTAGALARLLGGTDP
                     TGVVARASWLPPLVSSLAAALVAAWLARRFGPATGLLAGAGIALATDYVDRSRLAAID
                     HHFLEFPLVLGIVAAAAAATDSRSSRDAARGAALLALALAAALLTQTALLLAAALVLA
                     LVLVGREMPPRSRLAAAGGFLLAAAIVAFYRVAQPAGYPDNEWYLGLPHASALLAAAA
                     ACAVQYGLLARGAAFWTSAGLAVAVGLSVLAAIPHAPEALLAGSRFLGGNAWLRSIVE
                     FQPLFLGYEARFWPDLCLLGGGFLVTAAIAVTPGWRRGRRALLLAFALAYGIAAVSSR
                     RFLAIATPLCAITAAVAVHDLRAWGRTRLARAAAAVVVVPSLLLTAGRVIRPAPVVTS
                     DVFPLIRAAAFLGSRSASSPGAAVLGPWSWGHLFDVIGGQHVLLDNFGSVGGRTEFEN
                     ATAITLATREKFVSDFCRERRVRFIVLEDPLPYFSAHAEMSGFPKAAFSTRDGAGPTR
                     LMRATFWWRAYVEGGRIRPDLGPAGGRFRDFRLVLVESAPQKSEKRSTVQVWEFRPEG
                     GAPVLTSEKKTIPIARDRYRREKE"
     gene            complement(3186..>3657)
                     /locus_tag="VF958_07205"
     CDS             complement(3186..>3657)
                     /locus_tag="VF958_07205"
                     /inference="COORDINATES: protein motif:HMM:NF019934.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type II secretion system protein GspG"
                     /protein_id="HEX9148934.1"
                     /translation="RESGFTLIELLIVIAIIGILAAIAIPNLLNAVQRGKQKRSMSDM
                     RTMATAVEAYAVDNNVYPSGQCTGKFTAVTSVLASNSLSVLAPTYIANPVRRDGWSNY
                     FLYNTTSNNQEYGFRSLGRDGITTGISCITTTNFNDDILYSNGGFVQWPEGSQN"
BASE COUNT          550 a         1291 c         1260 g          556 t
ORIGIN      
        1 cccgcggcga gcaggggaaa aacgacccgc aggatgcgcc cgccggcccg gaatcggagc
       61 gtgtcgagac ccagcccggc gagcgccgcg acggcgaaaa tggtcgcgga gagcgccttc
      121 tccggatagc ggatccggtc gagcggcggg atcgcgcgaa gccacgaccc gggaggccct
      181 gcggccgcga tcaggagtcc cgcgattccg aaggcgagaa gaagcatcgc gagcgagcgc
      241 cgctcctttt cgaaaaacgc cgcggccgcc agcgcgaggg cgagcggccc gacgaagagt
      301 gacggcagcc agtacgtccg cgtcgcgccc ggcggccgcg cgaacgccgc ggcgagcgcc
      361 ggaatcggga tcgcgccggg ctcccgctcg gccacgggga gcggcccgcg acggtccgag
      421 tggagcgcga gctcccccat cgggacgagc acccacgcgg cgagggcgag cccgagcaga
      481 acggctcccg ccgtccgcac gaccgatctc ccgcgccggt cgagccacgg tcggtccgtc
      541 tcagcccggg gagagagcga caggacgagg gcgagaagaa gcgtcatcgc ggtcagctcc
      601 gggctcccgg ccatcgcctg gagccccacg agaagggcga gcgcgagcag cgccccgcgg
      661 gtccgcactc cggaccgggc gacccacgcg atcgccggga tccaggccca ggcgccgaag
      721 tggttccaga aggcggagag cgaggccgcg aagcccgacg cggcgaacac cgcggcggcg
      781 gcgagtgctc cggagtcgga gacgccctcc tgcttgcaga agcggtgcat cccccacacc
      841 gcgatcgcga agtggacgag cagaaacagg atcgccgcgg cgccgggcca cggcagcagg
      901 aaaaaagcgg tcggcgggta gaaaacgccg gcctgaccgt tggcgagcca gggttctccc
      961 gccccggaga gcgggttcca gagcggaatc cgaccggaga ggagacggtc cgccgtatgg
     1021 agcttcaacg ggaagaagaa gtccccctgg tcgcggaacg acggcgcccg acgcgcgagg
     1081 agtgccggtc cgagccagaa gagggaagga aggagcgcgg ccgccgcgag gactccggcg
     1141 aaccgtcggc gcgagggcac gagagattgt aaacggagag gcgccaatcg gacgcccgac
     1201 cgctcggggc ggcggtctac aatcgattcc gctcttgaac gtcgcttctc ccgcgttctt
     1261 cgcccggcac cgagcgttct tcgtcgccgg cgcggttttc ctcggagcat ttctcatccg
     1321 gacggcgaac ctgcgaacgg cgttcgtcgg cggggtgccc cagctttcgc ccttcgacga
     1381 gctgtaccac gcgaaacgga tcgtctattc ggccgatcgc ccgctgcgcg tgctgaactt
     1441 cgacccgagc cgcggaaccg ccggggcctt ttgtccgtgg ccgccgcttt acgacatgac
     1501 cgcaggcgct ctcgcgcggc tcctcggggg gacggatccg acgggcgtcg tggcgcgagc
     1561 gagctggctt ccaccgctcg tctcttcgct cgcggccgcc ctcgtcgcgg catggctcgc
     1621 gcggcgtttc ggtcctgcga cggggcttct cgcaggggca gggatcgctc tcgcgaccga
     1681 ctacgtcgat cgctcgcggc tcgcggcgat cgaccaccac ttcctggagt ttccactcgt
     1741 gctcgggatc gtcgccgcgg ccgccgccgc caccgactcg cgcagctccc gcgacgccgc
     1801 acgcggcgca gcgctcctcg ctctcgcgct cgccgcggct ctcctcaccc agacggcgct
     1861 cctcctcgcg gccgcgctcg tgctggcgct cgtcctcgtc ggccgcgaaa tgccgccacg
     1921 gtcccgtctc gccgccgccg gcgggtttct ccttgccgcc gcgatcgtcg cgttctaccg
     1981 ggtcgcgcaa cctgccggat acccggacaa cgagtggtac ctgggacttc cccacgcgtc
     2041 cgccctgctc gcggcggccg cggcgtgcgc cgtgcagtac ggcctgctcg cgcgcggcgc
     2101 cgcgttctgg acgtccgccg gacttgcggt ggccgtcggc ctttcggttc tcgccgcgat
     2161 cccgcacgcg cccgaagcgc tgctcgccgg gagccgtttc ctcggcggca acgcgtggtt
     2221 gcgctcgatc gtcgaattcc agcccctctt cctcggctac gaagcgcggt tctggccgga
     2281 cctctgtctg ctcggcggcg ggttcctcgt caccgcggcc atcgccgtaa cccccgggtg
     2341 gcggcggggc cggcgggccc tgctcctcgc gttcgccctg gcctacggga tcgcggctgt
     2401 cagctcccga cgctttctcg cgatcgcgac ccctctctgc gcgatcaccg cggcggtggc
     2461 cgtgcacgat ctgcgggcgt ggggccgaac ccggctcgcc cgcgccgccg cagccgtcgt
     2521 cgtcgtgccg tctctgctgc tgacggcggg ccgggtgatc cgtcccgctc cggtcgtcac
     2581 gagcgacgtg ttcccgctca ttcgcgccgc cgccttcctg ggaagccgaa gcgcatccag
     2641 ccccggcgcc gctgtcctcg gcccctggtc gtggggacac ctgttcgacg tcatcggcgg
     2701 ccagcacgtt ctcctcgaca acttcggttc ggtcggcggc cgcaccgagt tcgagaacgc
     2761 gaccgccatc acactcgcga ctcgcgaaaa gttcgtctcg gacttctgcc gggagaggag
     2821 agtccggttc atcgtcctcg aggacccgct cccctacttt tcagctcacg cggaaatgag
     2881 cggcttcccg aaggcggcgt tctcgactcg ggatggcgcc ggacccacgc gcctcatgcg
     2941 agccaccttc tggtggcggg cctacgtcga ggggggacga atccgtccgg acctcggccc
     3001 cgccggcggc cggttccgcg acttccggct ggtgctcgtc gagtcggctc cgcagaagtc
     3061 ggaaaagcga tccacggttc aggtctggga attccgaccg gaaggcggag caccagttct
     3121 gacgagcgaa aaaaaaacga tcccgattgc tcgggaccgt tacaggagag agaaggaatg
     3181 agttcttagt tctgggagcc ttccggccac tggacgaagc cgccgttcga atagaggatg
     3241 tcatcgttga agttggtcgt cgtgatgcag ctgataccag ttgtgatgcc gtcccgaccc
     3301 agcgagcgga atccgtactc ctggttgttc gacgtggtgt tgtagaggaa gtaattgctc
     3361 cagccgtctc tccgcaccgg gttggcgatg taggtcgggg ccaggaccga aaggctgttg
     3421 ctggccagga ccgacgtgac ggccgtgaac ttgccggtgc actgacccga cgggtacacg
     3481 ttgttgtcga ccgcgtaggc ctcgaccgcg gtcgccatcg tgcgcatgtc ggacatcgaa
     3541 cgcttctgct tgccacgctg gaccgcgttc agaaggttcg gaatcgcgat cgccgcgagg
     3601 atgccgatga tcgcgatgac gatcaacagc tcgatcagag tgaaaccaga ttcccgc
//
LOCUS       DASWAV010000347         3739 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U2665
            k141_19750, whole genome shotgun sequence.
ACCESSION   DASWAV010000347 DASWAV010000000
VERSION     DASWAV010000347.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960316
            Sequence Read Archive: SRR10260007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 3739)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3739)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:47:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,929
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3739
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_19750"
                     /isolate="SMAG_U2665"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.36444 N 132.31889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(128..1927)
                     /locus_tag="VF958_07210"
     CDS             complement(128..1927)
                     /locus_tag="VF958_07210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013119154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04190 family B12-binding domain/radical SAM
                     domain protein"
                     /protein_id="HEX9148935.1"
                     /translation="MRLFGTDLVLLHPPSVYDFRKTRSEYGPISDVIFSSPAFEMYPI
                     GLTSIASHLEEQGHNVRIVNVAHRMLSDPGYDAEAAIARLHPAVFGIGLHWLPHAHGA
                     LELARIVRRHHPATPIVFGGISASYFHEELIRSPFVDFVLRGDSAEEPMLQLLRALQL
                     GGRLEDIPNLTWKRDGDAVVNPLTYVPSRLDGPAIPDVFYAIRSVFKYRSLADVVPFT
                     GWLEYPITGLLTARGCSMACAVCGGSRPAYRAICNRPGPAFRAPEALVRDIHRIRKFS
                     RGPIFLIHDLRQAGRAWTEKVLSLLGRERPRNEIVLELFSPVADDYLERIGAALPRWS
                     VEITLESHLERIRALNRRFDCSNEAIESTIRKALENGAGRVDLFFLVGLPGQSSSDAV
                     GCVDFCRSLLETFRGDRRLSFFVAPLAPFLDPGSEAYEKPESSGYRVRFRTLDEYSTA
                     LTAPSWKHMLNYETEWMTRDEIVAATYEALRRMTRLKREWGQIDGEVSEAAVAAIDAS
                     EKAVSAIDDALRLPVGPERESVLAEAQRGASTPSAAARFQKNALVWPIPGSHRFAPLV
                     SLAAVAVELAFFEARLLLTRRLPLYLSQPRHVG"
     gene            complement(1983..3182)
                     /locus_tag="VF958_07215"
     CDS             complement(1983..3182)
                     /locus_tag="VF958_07215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009019994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX9148936.1"
                     /translation="MQRDLWKLAWSSISAHPLRSSLTILGIVIGIASVILLTSLGEGT
                     RRSLGAEFSQFGTNVMAIHRGKTTTSGIPGAVGGTIRELTIEDSEALRRVPGVERVVP
                     VAWGSARVEAGERGRSVFVYGVTSEVPAVWKFPVRQGRFLPAGDPRRAASLAVLGPKL
                     KHELFGDANALGERIRVAGRRFVVIGVMAPKGQLFGFDMDDTVYIPVASAKTLFNTEE
                     LIEIDVLFSLGIPVNRVKEGVREELMRRHGGEEDFTVSTQTEALDVLNRVMKIVTLAV
                     GAIGGISLVVGAIGILTMMWIAVRERTGEIGLVKAIGAAPSQIARLFLLEAALLSLAG
                     GVVGVLVGILVGALLGWAVPALSVHVAPGFVVAALLVSLAVGIAGGVLPARRAARLDP
                     LEALRAE"
     gene            complement(3186..>3739)
                     /locus_tag="VF958_07220"
     CDS             complement(3186..>3739)
                     /locus_tag="VF958_07220"
                     /inference="COORDINATES: protein motif:HMM:NF014717.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HEX9148937.1"
                     /translation="TSLFRVLVEISSHEEIPRAKTAVLRLLTERHGGEEDVTVWTQDS
                     VVATFSRILALLTATLAGIAAISLTVAGVGIMNVMLVSVSERTREIGLLKAIGVTGRQ
                     VVAAFLVEAAIISTAGGAVGLAAASAGVGLLRLLYPSFPVHAPEWAIPAAVGLALGVG
                     LLFGVAPARRAARLDPITALARR"
BASE COUNT          650 a         1289 c         1277 g          523 t
ORIGIN      
        1 cggtccctcc ctcgctcccg ctcccgcgcc gcgaggctcc tcacttcgat tacctcagga
       61 gactcctcgc gccgcgaaaa tgggcacaga cccctttagc ccccgaactg atcgcgcccc
      121 cgtcgcgtca accgacgtgc cggggctggg ataggtagag cggcagccgc cgggtcagga
      181 gcagccgcgc ctcgaagaaa gccagctcga cggcgaccgc cgcgagggac acgagcggtg
      241 cgaaccggtg gcttcccgga atcggccaca cgagggcgtt cttctggaac cgcgcggccg
      301 ccgaaggggt tgaagcgcct cgctgcgctt ctgcgagcac cgactcgcgc tcgggcccga
      361 ccggcaggcg gagggcatcg tcgatcgccg agaccgcctt ctcgctcgcg tcgatcgccg
      421 cgaccgcagc ttccgagact tccccatcga tctggcccca ctcgcgcttc aaccgcgtca
      481 tccgccgcag agcctcgtac gtggcggcga cgatctcgtc gcgcgtcatc cactccgtct
      541 cgtagttcaa catgtgcttc cagctcggcg cggtcaaggc ggtgctgtac tcgtcgaggg
      601 tccggaaccg gacccggtaa ccggagctct ccggcttctc gtaggcctcg cttcccgggt
      661 cgaggaaggg agcgaggggc gcgacgaaga acgagagcct ccggtcgccg cggaacgttt
      721 ccaggagtga ccggcagaag tcgacgcagc cgacggcgtc cgaggagctc tgccccggga
      781 gccccaccag gaagaagaga tccacccggc ccgcgccgtt ctccagggcc ttgcggatcg
      841 tcgactcgat cgcctcgttg gagcaatcga agcgccggtt caacgcgcgg atgcgctcga
      901 gatgggactc gagcgtgatc tcgacgctcc accgcggcag ggccgcgccg atgcgctcga
      961 gatagtcgtc ggccaccggc gagaagagct ccagcacgat ctcgtttcga gggcgttcgc
     1021 gcccgagcag agagaggacc ttctcggtcc acgcgcggcc ggcctggcga agatcgtgga
     1081 tgaggaagat cggcccgcga ctgaatttcc ggatccgatg gatgtcccgc acgagggcct
     1141 ccggcgcccg aaatgccggc ccggggcggt tgcagatcgc ccggtaggcc ggtctcgagc
     1201 cgccgcagac ggcgcaggcc atcgagcagc cgcgggcggt caggagaccc gtgatcgggt
     1261 actcgagcca gccggtgaac gggacgacgt cggccaggct gcggtacttg aagaccgatc
     1321 ggatcgcgta gaagacgtcc gggatcgccg gaccgtccag gcgagacggc acgtaggtca
     1381 gggggttcac gacggcgtcg ccgtcgcgct tccacgtgag gttggggatg tcctcgagtc
     1441 tcccgccgag ctgaagcgcg cggagcagct ggagcatcgg ctcctccgcc gagtcgccgc
     1501 gcaggacgaa gtccacgaac ggcgagcgaa tcagctcttc gtggaaatac gacgccgaga
     1561 tcccgccgaa gacgatcgga gtcgcgggat ggtgtcgccg gacgatcctt gcgagctcca
     1621 gcgcgccgtg cgcgtgcggg agccagtgga gcccgattcc gaacaccgcc gggtggagcc
     1681 gcgcgatggc ggcctcggcg tcgtagccgg ggtcggacag cattcggtgg gcgacgttca
     1741 cgattcgcac gttgtggccc tgctcttcga gatggctcgc gatcgacgtc aggccgatcg
     1801 ggtacatctc gaacgccggc gacgagaaga tgacgtcgct gatgggcccg tattcggacc
     1861 gcgtcttgcg gaagtcgtag acgctcggcg gatgcagaag gacgaggtcg gttccgaaga
     1921 ggcgcattcc gatcgattcc ctccggctgt tcggaggaac gattatcgcc ggaggctccg
     1981 gactactcgg cccgcagggc ctcgagagga tcgagtcggg cggcccggcg ggccgggaga
     2041 acgccgccgg cgattcccac cgcgaggctg acgaggagag cggcgacgac gaagcccggc
     2101 gcgacgtgaa ccgaaagcgc cggcaccgcc cacccgagga gcgccccgac gaggattccc
     2161 acgagcacgc ccaccacgcc gccggcgagg gacaggagcg cggcctcgag caggaacagc
     2221 cgggcgatct gcgacggagc ggcgccgatc gccttgacga gtccgatttc cccggtgcgt
     2281 tcccggacgg caatccacat catcgtcagg atcccgatcg ctccgaccac gagcgagatc
     2341 ccgccgatcg ctccgacggc gagcgtgacg attttcatca cccggttcag gacgtcgagg
     2401 gcctcggtct gggtcgagac ggtgaaatcc tcttctccgc cgtggcggcg catcagctcc
     2461 tcgcgcacgc cctccttcac gcggttgaca ggaatcccga gtgagaagag aacgtcgatc
     2521 tcgatcagct cttcggtgtt gaagagggtc ttcgcgctcg ccaccgggat gtacaccgtg
     2581 tcgtccatgt cgaacccgaa cagctgcccc ttcggggcca tcactccgat gacgacgaag
     2641 cgccgcccgg cgacccgaat gcgctcgccg agagcgttcg cgtcaccgaa cagctcgtgt
     2701 ttcagcttcg gaccgagcac cgccagagac gcggcccggc gaggatcgcc cgccggaaga
     2761 aagcggccct gacgcaccgg gaacttccag acggccggaa cttccgaggt cactccgtag
     2821 acgaacacgc tgcggccccg ttcccccgcc tcgacgcggg cggagcccca ggcgacgggc
     2881 acgactcgct cgacgcccgg gacacgacgc agagcctccg aatcctcgat cgtcagctcg
     2941 cggatggtcc ccccgaccgc tccggggatc ccgctcgtgg tcgtcttgcc tcggtggatc
     3001 gccatcacgt tcgtaccgaa ctgggagaac tcggcgccga gagaacgccg cgttccctca
     3061 ccgagcgacg tcagcaggat cacggacgcg atgccgatca cgatgccgag gatggtgagg
     3121 gacgagcgga gcgggtgggc tgaaatcgag ctccaggcca gtttccagag gtcacgctgc
     3181 acggctcacc tcctcgcaag cgccgtgatc ggatcgaggc gcgcggcgcg acgcgcaggc
     3241 gcgacgccga agaggaggcc gacgccgagc gcgagaccga cggccgcggg gatcgcccac
     3301 tccggcgcgt ggacgggaaa actcggatag aggagacgca ggagccccac cccggcggag
     3361 gcggccgcga ggccgacggc gccgcccgcc gtcgagatga tcgcggcctc gaccaggaat
     3421 gccgcgacga cctgccgtcc ggtcacgccg atcgccttga gcagcccgat ctcccgggtc
     3481 cgttccgaga ccgacaccag catcacgttc atgatcccga ccccggcgac cgtgagcgag
     3541 atcgccgcga tcccggcgag cgtcgcggtc aggagcgcga ggatccggga aaacgtcgcc
     3601 acgacggagt cctgcgtcca gaccgtcacg tcctcttcgc caccgtgacg ttcagtcagg
     3661 agccggagta cggcggtctt cgcccgcgga atctcctcgt gcgaggagat ctcgacgagc
     3721 acccggaaga ggctcgtcc
//
LOCUS       DASWAV010000348         8267 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U2665
            k141_218502, whole genome shotgun sequence.
ACCESSION   DASWAV010000348 DASWAV010000000
VERSION     DASWAV010000348.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960316
            Sequence Read Archive: SRR10260007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 8267)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8267)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:47:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,929
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8267
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_218502"
                     /isolate="SMAG_U2665"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.36444 N 132.31889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..387)
                     /locus_tag="VF958_07225"
     CDS             complement(<1..387)
                     /locus_tag="VF958_07225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9148938.1"
                     /translation="MMIARVRTVDAILDQVGRFEAIRKADLRRGDRVLVVTENSIYCI
                     HVEEEGSYSVSGGWFDRNGMSPVTLTISGCTWGGTAIKRDLVAARGLRLEFGNRVVTS
                     PIRDVQVIRGEARVLNGMQAISTAELF"
     gene            complement(519..1898)
                     /locus_tag="VF958_07230"
     CDS             complement(519..1898)
                     /locus_tag="VF958_07230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011688899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEX9148939.1"
                     /translation="MYFKQFYLGCLAHASYMIGSDGEAAVVDPRRDVDSYIDEAREQG
                     LEIKHVIETHLHADFVSGHRELAHRTGAKIYFGAKAGAKFEHVPVREGDEIRMGDVVL
                     RFLETPGHTPESVSILVVDRAVSNVPKAVLTGDTLFIGDVGRPDLLGARMSAQELAGM
                     LYDSLHEKLLALPDSVEVYPAHGAGSLCGRNISSETSSTIGQQRQFNYALRPMPKEEF
                     VRMMTTDLPEAPAYFSRDVAINRDGASELAELPDPAALSARDVEALQKRGAVILDTRP
                     AAQFGAGHLPGSLHIGLSGQFASWAGALISPRSPIVFVAEEEEQIREARTRLARVGIE
                     NVAGYLAGGILSWHDAGLPLATTEQIGVEELDARMREGTFGRVVDVRRPGEWQAGHIP
                     NALNLPLNTLAGDAAALPKGQPLAVICAGGFRSSIATSILEQQGFTKVTNVVGGMAAW
                     RNANLEVAA"
     gene            complement(1976..2404)
                     /locus_tag="VF958_07235"
     CDS             complement(1976..2404)
                     /locus_tag="VF958_07235"
                     /EC_number="4.1.2.50"
                     /inference="COORDINATES: protein motif:HMM:NF013411.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070497 -
                     6-carboxy-5,6,7,8-tetrahydropterin synthase activity
                     [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase"
                     /protein_id="HEX9148940.1"
                     /translation="MIRLSRTIHFNAGRSLWRKDWTPEKNRAVYGEESPHGYGHNFTL
                     EVAVEGDIDPESAMVVNLTDLDRILKEEVDAPLDHKNLNHDVPGFEKLPPTAENVAAW
                     IWRRVSARIEREGWPCRLAHLRLTLTPDLAVEIEERPQPV"
     gene            2509..3930
                     /locus_tag="VF958_07240"
     CDS             2509..3930
                     /locus_tag="VF958_07240"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012872429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase"
                     /protein_id="HEX9148941.1"
                     /translation="MNFYSTAEELEEDLERSGVIRREGILVIASPDRFRTQTIDGLVW
                     TAVFGVREVKAAARSAIRQAAASLGILPASILPLYEARGRGEISGFTVPAVNIRLLAY
                     DTARAACRAARKLDAGAVIFEIARSEIGYTDQRPGEYAAVVLGAAIREGWEGPVFLQG
                     DHFQTNPKKMEADPDKEIAAIEALIEEAIAASFYQIDIDTSTLVDLSKPTLAEQQAVN
                     SKLTARFTKFIRAHEPKDMPISIGGEIGEVGKENSTPDELRAYMSEYHRELGGAKGIA
                     KVSVQTGSSHGGVVGPDGTVQRVQIDFETLRRISKVAREEFGLAGAVQHGASTLPAEF
                     FGSFPSHDCAEIHLATEFQNMVYDHPVLPLPLKREVERWLFQRMADQKKKGETESQFL
                     YKNRKQAIGAFKEQFWNLPPGTRESIRDTLEKKFAFLFEKLGITRTRDLVARYVKPAA
                     ALPELAGVTGPRAGYVRDDEAGD"
     gene            4040..5122
                     /locus_tag="VF958_07245"
     CDS             4040..5122
                     /locus_tag="VF958_07245"
                     /inference="COORDINATES: protein motif:HMM:NF012335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HEX9148942.1"
                     /translation="MTAVVKTKPVAGRDGTEVKSVPVPVPGPGEVLIRVLATAACGTD
                     RHIYKWDESMASIVKPPRIYGHEFCGEIAGFGPDADRQHLREGLYVSAEMHVTCGHCR
                     PCRTGQRHICENTRILGVHGDGCFAQYVVVPAYNVIPLDRHVVQPRVGAFLDALGNAV
                     HTTQVADLSGKSVAILGYGPIGAMCAEIARISGAARIAITEINAQAAAHARRWASSRR
                     LSQVSVIELNRTKDAAGSVREALGGGADVVLELSGAESSINLGLAVARSGAVVSLLGI
                     PKESAVTIRDYTRCLIFKGLTLHAVIGRRIFETWIKMLDLLSAGLDVEDIVTDEFDGL
                     EKFHEAMALLESRRAMKVVFYPNGRP"
     gene            5155..5622
                     /locus_tag="VF958_07250"
     CDS             5155..5622
                     /locus_tag="VF958_07250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9148943.1"
                     /translation="MPTNPPPKPDVSEGMNERRIAEFALEESRLSNVSYRFRVTPRDR
                     VWKNWARTEFEPNRYRVLGLAGVCLSRDETDEAQKIASRALGDLDDILYTVERPARAV
                     IVQGASGTSEIINRPTLCAEVIAPNAAAFERALSALDAAFPGGRVAGKILSEE"
     gene            5633..6814
                     /locus_tag="VF958_07255"
     CDS             5633..6814
                     /locus_tag="VF958_07255"
                     /EC_number="2.3.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015774853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008890 - glycine C-acetyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine C-acetyltransferase"
                     /protein_id="HEX9148944.1"
                     /translation="MTTLVDQLAAEMAKLREAGTYKEELVLESPQDAKVRVGGREVVM
                     LTSNNYLGFANHPRVREAQKKAIDRWGAGMASVRFICGTEQLHKELEAEIAGFFGADD
                     SILYMSCWNANEGLFAAILGETDGLYSDELNHASIIDGIRLCKAKRFLVPHNDTAAFE
                     RMLGEDSTSRFRLMITDGVFSMEGEEANLRELVRICQAKNVLLAVDDSHATGVLGETG
                     RGTAEEQGVFDRIPVTTGTLGKAMGSAAGGFVAGPKALVETLRQRSRTYLFSNSLPPA
                     VAAGSLEAFRMLREDPAPVRKLRDNAAYFRQAMTEAGFRIPHGSHPIVPVIVGDTAKA
                     LAMGKSLFEEGIYVSGFGYPVVPQGQARLRCQVSAAHERADLDRAVEAFRKVGKKFDV
                     A"
     gene            6824..7681
                     /locus_tag="VF958_07260"
     CDS             6824..7681
                     /locus_tag="VF958_07260"
                     /inference="COORDINATES: protein motif:HMM:NF042511.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6544 family protein"
                     /protein_id="HEX9148945.1"
                     /translation="MKIAGRIVAGLLLAAGGLLLSAELRWRRKTAALVRRLGSGGSVT
                     PPVGFHSARLENLPLPVRRYFAAVLREGQPAIRRARLRQRGEFLVKPEGNRWAPFDAV
                     QHIAVLPPGFVWDARIRMAPGLKVRVRDGFVEGQGTMSASVMGVKTILRVENTPDIAA
                     AALMRYLAETTWVPTALLPSAGVSWTSIDDSSARATLTAGPTTVALDFRFGADGLVES
                     VFSPARGREVGGSTIPTPWQGRWTCWENQGGIRIPVEGEVEWLLPEGPQPYWRGRVVE
                     VAYDFGSED"
     gene            7720..>8267
                     /locus_tag="VF958_07265"
     CDS             7720..>8267
                     /locus_tag="VF958_07265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9148946.1"
                     /translation="MRERHAVTVGAPAAFVLDVARNFDPQSIPAVRVIFAVREKVMGA
                     RVDRGDLPTGLVPWTLAMGWGALADEPGRFFVAGAACQPWKADVDFMPIPSERFASYA
                     EPDRVKIAWTLETEPLGPGTSRFASETRAVATDSAAREKFRRYWRFARFGIVAIRWLL
                     RPGVRREAERRGHFREADRPIDS"
BASE COUNT         1398 a         2721 c         2797 g         1351 t
ORIGIN      
        1 gaagagttcg gcggtggaga tcgcttgcat gccgttcagg actcgggctt ctccgcggat
       61 gacctggacg tcccggatcg ggctcgtcac gactcggttg ccgaactcga gccggagacc
      121 gcgggcggcg acgaggtcac gcttgatcgc cgtgccgccc cacgtgcacc cgctgatggt
      181 gagggtgacg ggcgacattc cattccgatc gaaccagcct ccggagaccg aatagctccc
      241 ctcttcttcg acgtgaatgc agtagatcga attctccgtc acgacgagca cgcggtcacc
      301 gcggcgcagg tccgccttcc ggatggcctc gaagcggccc acctgatcga ggatggcgtc
      361 aacggttcgt acgcgggcga tcatcattgg gtagtcctga gtcccaagtc ccgacaccaa
      421 gtttaagcct acgcaaaaga gggccggatt cggcggtccc tgcgatatgg cgcgtcgtcc
      481 gccgttctcc gtgcatggac tccgcaagtc caatacacct aggccgcgac ctcgaggttc
      541 gcgttccgcc acgccgccat cccgccgacg acgttcgtaa ccttcgtgaa gccctgctgc
      601 tcgaggatgc tcgtcgcaat cgaggagcga aagccgccgg cgcagatgac ggcgaggggc
      661 tggccctttg gaagcgcagc cgcgtcgccc gccagagtgt tcagcggcag gttcaaggcg
      721 ttcggaatat gccctgcctg ccactcgccc ggccggcgga cgtccacgac tcttccgaac
      781 gtaccttccc gcatccgggc atcgagctcc tcgacgccga tctgctcggt cgtcgcgagc
      841 ggcagccccg cgtcgtgcca cgagaggatc ccgccggcga gataacccgc cacgttctcg
      901 atccccacgc gcgcgagccg cgtccgcgcc tcgcgaatct gctcctcctc ctcggcgacg
      961 aacacgatcg gcgagcgggg cgagatgagc gccccggccc aggaggcgaa ctgccccgag
     1021 aggccgatgt ggagcgatcc gggcaagtgc cccgcgccga actgcgcggc cggacgcgtg
     1081 tcgaggatga cggcgcccct tttctggaga gcttcgacgt cgcgagccga cagtgcggcg
     1141 ggatccggga gttcggcgag ctcggatgcc ccgtcccgat tgatggcgac gtcgcgcgag
     1201 aaatatgccg gcgcctccgg gagatccgtc gtcatcatcc ggacgaactc ttctttcggc
     1261 atcggccgca gtgcgtagtt gaactgccgc tgctggccga tcgtggagct cgtctcgctc
     1321 gagatgttcc ggccgcagag cgagcccgcg ccatgcgccg gatagacctc caccgagtcg
     1381 ggcaacgcca ggagcttctc gtgaagcgag tcgtagagca tccccgcgag ctcctgtgcg
     1441 ctcatcctcg cgccgagaag gtcggggcgt ccgacgtcgc cgatgaagag cgtgtcgccg
     1501 gtcaggacgg ctttggggac gttggagacc gcgcgatcga cgacgaggat cgaaaccgac
     1561 tccggcgtgt ggccgggggt ctcgaggaag cgcaagacga cgtcacccat ccgaatctcg
     1621 tcgccctccc gcaccgggac gtgctcgaac ttcgcgcccg ccttcgctcc gaaatagatc
     1681 ttcgcgccgg tgcggtgcgc gagctcgcgg tggccggaga cgaagtcggc gtgcagatga
     1741 gtctcgatga cgtgtttgat ctcgagaccc tgttcccgcg cctcgtcgat gtagctgtcg
     1801 acgtcgcgcc gggggtcgac gaccgccgcc tcgccgtccg atccgatcat gtaggaagcg
     1861 tgagcgagac agcccaagta gaactgtttg aagtacatcc tttgatgcta tctcttgatc
     1921 gccgaaggtt gcccctctcc ctccgggaga gggggccggg ggtgaggggg ttgactcaga
     1981 cgggctgtgg acgctcttcg atctccaccg cgagatccgg cgtcagggtg agcctcaggt
     2041 gcgccaggcg gcatggccag ccttcgcgct cgatccgcgc cgagacccgg cgccagatcc
     2101 aggcggcgac gttctccgcg gtcggcggaa gtttttcgaa cccgggaacg tcgtggttga
     2161 gattcttgtg gtcgagcggc gcgtccacct cttccttgag gatccgatcc aggtccgtca
     2221 ggttgacgac catcgccgac tccggatcga tgtcgccctc caccgccact tcgagagtga
     2281 agttatggcc gtagccgtgg gggctctctt ccccgtagac cgcgcggttc ttctccggag
     2341 tccagtcctt cctccagagg ctgcggccgg cgttgaagtg gatcgtccgc gagaggcgga
     2401 tcacgccgcg aacctagcac acggctcatg tccggaaacg accctcgtcg gctcgatgtg
     2461 gcaagtggag acctgacccc atagaatccc ggcgccgcga aggacgtcat gaatttctac
     2521 tcgaccgcag aagagctcga agaggacctc gaacgttcgg gcgtgatccg ccgcgaaggg
     2581 attctcgtca tcgcgagccc cgaccgtttc cgcacgcaga cgatcgacgg gctggtctgg
     2641 acggccgtgt tcggggttcg cgaagtcaag gccgctgccc gaagcgcgat ccggcaggcg
     2701 gcggcgtcgc tcggcatcct tccggcgtcc atccttccgc tctacgaggc gcgggggcgc
     2761 ggcgagatct cgggcttcac agttccggcc gtcaacatcc gcctgctcgc ttacgacacc
     2821 gcgcgcgccg cgtgccgcgc ggcgcgaaag ctcgacgcgg gcgccgtgat cttcgagatc
     2881 gcgcgcagcg aaatcggata cacggaccag cgccccgggg aatacgccgc cgtcgttctc
     2941 ggcgcggcga tccgggaggg ctgggaaggg ccggtcttcc tccagggcga ccatttccag
     3001 acgaacccga agaagatgga ggcggacccg gacaaggaga tcgcggcgat cgaagcgttg
     3061 atcgaggagg cgatcgcggc gagcttctac cagatcgaca tcgatacctc gacgctcgtc
     3121 gacctctcga aaccgacgct cgccgagcag caggcggtga actcgaagct gacggcgcgg
     3181 ttcacgaagt tcatccgcgc gcacgagccg aaggacatgc cgatctcgat cggcggggag
     3241 atcggcgaag tcggcaagga gaactcgacg cccgacgagc tgcgcgcgta catgagcgaa
     3301 taccatcggg agctcggcgg tgccaaaggc atcgcaaaag tgtcggtgca gaccggcagc
     3361 tcgcacggcg gagtggtcgg acctgacgga accgtccagc gcgttcagat cgatttcgag
     3421 accctgcgcc gcatctcgaa agtcgctcgc gaggaattcg ggctcgcggg cgcggtccag
     3481 cacggcgcgt ccacgctgcc ggcggagttc ttcggcagct tcccctcgca cgactgcgcc
     3541 gagatccacc tcgcgaccga gttccagaac atggtctacg accaccccgt tctgccgctg
     3601 ccgctcaagc gcgaggtcga acgctggctg ttccagagaa tggccgatca gaagaagaag
     3661 ggcgagaccg agagtcagtt tctctacaag aaccgcaagc aggcgatcgg ggcgttcaaa
     3721 gagcagttct ggaacctccc gcccggaacg cgggagtcga tccgcgacac gctcgagaag
     3781 aagttcgcct ttctcttcga aaagctcggc atcacgcgca cccgggatct cgtggcgcga
     3841 tacgtcaagc cggccgccgc tcttcccgag cttgccggcg tcaccggtcc gcgcgccgga
     3901 tacgtgcgcg acgacgaggc aggcgattga ggctgcggaa atcgggaatc ggaaatcggg
     3961 aatcgtgaag aggacgagaa ggatcccgtc gcgctcgaag tctcgatcaa agacgaccga
     4021 ttcccgactc ccgacccgga tgaccgccgt cgtcaagacg aagccggtcg caggccggga
     4081 cgggacggag gtcaagtcgg ttcccgtgcc ggtgccggga cccggcgaag tgttgatccg
     4141 cgttctggcg accgccgcgt gcggcaccga ccggcacatc tacaagtggg acgagtcgat
     4201 ggcttcgatc gtcaagccac cgcggatcta cgggcacgag ttctgcggcg agatcgcggg
     4261 attcggaccg gacgcggacc ggcagcacct gcgcgagggg ctgtacgtct ccgcggagat
     4321 gcacgtcacc tgcggccact gccgtccctg ccgcacgggg cagcgccaca tctgtgagaa
     4381 cacgcgcatc ctcggcgtgc acggggacgg ctgcttcgcg cagtacgtcg tcgttccggc
     4441 atacaacgtg atcccgctcg accggcacgt cgtgcagccg cgcgtcggcg cgtttctcga
     4501 tgcgctggga aacgcggttc acacgaccca ggtggccgac ctctccggga agtccgtcgc
     4561 gattctcggc tacgggccca tcggcgccat gtgcgccgag atcgcgcgga tctccggcgc
     4621 ggcgcggatc gcgatcaccg agatcaacgc gcaggccgcg gcgcacgccc ggcggtgggc
     4681 gagctcccgg cgtctttcgc aggtgtcggt catcgagctc aaccgcacga aagacgcggc
     4741 ggggagcgtg cgcgaagcgc tcggcggagg cgcggacgtc gtgctcgagc tctccggcgc
     4801 ggaatcttcg atcaacctcg ggctcgcagt cgcgcggagt ggcgcagtcg tttcgctcct
     4861 ggggatcccg aaggagagcg cggtgacgat ccgcgactac acgcggtgcc tgatcttcaa
     4921 gggcctgaca ctccacgcgg tgatcggccg gcgtatcttc gaaacgtgga tcaaaatgct
     4981 cgatctcctt tccgccggcc tggacgtcga agacatagtc accgacgagt tcgacggact
     5041 cgagaagttc cacgaggcca tggcactgct cgagagccgc cgagcgatga aggtcgtctt
     5101 ctatccgaac ggaagaccgt agcgagcctg cgccgcaagt agacggagaa atcaatgccg
     5161 accaatccgc cgcccaagcc agatgtctcg gagggaatga acgagcgccg catcgccgag
     5221 ttcgccctgg aagagtcccg cctctcgaac gtctcctacc ggttccgcgt cactccgcgc
     5281 gatcgcgtgt ggaagaactg ggcccgcacg gagttcgagc cgaaccgcta ccgggtgctc
     5341 ggactcgcgg gggtctgcct ttcccgcgac gagaccgacg aagcgcagaa gatcgcgtcg
     5401 cgcgctctcg gcgatctcga cgacatcctc tacacggtcg agcgaccggc tcgcgcggtc
     5461 atcgtgcagg gagcgagcgg gacgtccgag atcatcaacc gcccgacgct gtgcgccgag
     5521 gtcattgccc ccaacgctgc cgcgttcgag cgggccctgt ctgcgctcga cgccgcgttc
     5581 cccggcggcc gcgtcgccgg gaagatcctg tcggaggagt gactcgcgca agatgaccac
     5641 gctcgtcgat cagctcgccg ccgagatggc gaaactgcgt gaggccggca cgtacaaaga
     5701 agagctcgtc ctcgagtcgc cgcaggacgc gaaggtccgc gtcggcggcc gcgaggtcgt
     5761 gatgctcact tcgaacaact atctgggttt cgccaaccat ccgcgggtcc gcgaggccca
     5821 gaagaaggca atcgatcgat ggggcgccgg gatggcgtcg gtgcggttca tctgcggaac
     5881 ggagcagctg cacaaggaac tcgaggcgga gatcgccggc ttcttcgggg ccgacgactc
     5941 cattctctac atgtcttgct ggaacgcgaa cgagggactg ttcgcagcca ttctcggcga
     6001 gaccgacggc ctctactcgg acgaactcaa tcacgcctcg atcatcgacg ggatccggct
     6061 gtgcaaggcg aagcggtttc tggttcctca caacgacacg gcggcgttcg aacggatgct
     6121 gggcgaggac tcgacgtccc gttttcgcct gatgatcacc gacggcgtct tctcgatgga
     6181 gggggaggag gcgaacctgc gtgagctcgt gcgtatctgc caggcgaaga acgtgctcct
     6241 cgcggtcgac gactcgcatg cgacgggcgt gctcggggag accggacgcg gcacggcgga
     6301 ggagcagggc gtattcgacc gcatccccgt gacgaccggc acgctcggaa aggcgatggg
     6361 atcggcggcg ggagggttcg tcgccggacc gaaggcgctc gtcgagacgc tccggcagcg
     6421 cagccgcacg tacctttttt cgaactcgct cccgcccgcc gtcgcggccg gctcgctcga
     6481 ggcgttccgg atgctgcgcg aggatcccgc gccggtgcgc aaactgcgcg acaacgcggc
     6541 gtacttccgc caggcgatga ccgaagccgg cttccggatc ccgcacgggt cgcacccgat
     6601 cgtccccgtg atcgtcggcg acaccgcgaa ggcgctcgcg atgggcaagt cgctcttcga
     6661 ggaaggaatc tacgtttccg gtttcggata cccggtcgtg ccgcaggggc aggcgcgact
     6721 gcgctgtcag gtctccgcgg cgcacgagcg ggcagacctc gatcgcgcgg tcgaagcttt
     6781 ccggaaggtc ggaaagaagt tcgacgtcgc ctaacggtca gccatgaaga tcgcgggtcg
     6841 gatcgtcgcg ggactcctgc tggcggccgg cggattgctc ctgtcggcgg agcttcgctg
     6901 gaggagaaag accgcggccc tcgtccggcg actcgggagt ggcggatccg tgacgccgcc
     6961 ggtcggattc cattccgctc gactcgagaa tctcccgctt ccggttcgac gctatttcgc
     7021 ggcggtgctc cgagagggtc agccggcgat ccggcgagcg cggctgcgac aacgcgggga
     7081 gtttctcgtc aagcccgagg gaaaccggtg ggcgccgttc gacgccgtcc agcacatcgc
     7141 cgtcctgccc cccggcttcg tctgggacgc gcggatccgg atggcgccgg gcctcaaggt
     7201 ccgcgttcgc gacggcttcg tcgaaggtca agggacgatg tcggcgagcg tgatgggggt
     7261 caagacgatc ctccgcgtcg agaacacacc cgacatcgcg gccgccgcgc tcatgcgata
     7321 tctcgccgag acgacctggg tcccgacggc gctgcttccc tcggccggag tctcctggac
     7381 ttcgatcgac gactcgagtg cccgcgcaac gttgacggcg gggcccacga ccgtggcgct
     7441 cgactttcga ttcggtgcgg acgggctggt cgaaagcgtt ttctctcctg cgcgcgggcg
     7501 tgaagtgggc ggctcgacga ttccgactcc ctggcaggga cggtggacgt gctgggaaaa
     7561 ccagggcgga attcgcattc ccgtcgaagg cgaggtcgag tggctgctgc cggaagggcc
     7621 gcagccctac tggcgcggcc gggtcgtcga ggtcgcctac gatttcggga gcgaagactg
     7681 acgccgacgc cgctcgagcg cttcctcccg gagttcgacg tgcgcgaacg ccacgcggtc
     7741 accgtgggcg ctcccgcggc attcgtcctc gacgtcgcgc gcaacttcga tccgcagtcg
     7801 attccggccg tgcgggtgat cttcgccgtg cgcgagaagg tcatgggcgc ccgagtggac
     7861 cggggcgacc tgccgacggg tctcgttccc tggacgctcg ccatgggctg gggggctctc
     7921 gccgacgagc ccggccgatt tttcgtcgcg ggcgcggcgt gccagccatg gaaagccgac
     7981 gtcgacttca tgccgatccc gtccgaacga ttcgcttctt acgccgagcc cgatcgggtg
     8041 aagatcgcgt ggacactcga gaccgagcct ctcggtcctg ggacgagccg gttcgcctcc
     8101 gagacccgcg cggtcgccac ggactccgcc gcgcgcgaga aattccgccg ttactggagg
     8161 ttcgcccgat tcgggatcgt tgcgatccgc tggctcctcc gtccgggcgt gcgccgggaa
     8221 gccgagcggc gggggcactt tcgagaagcg gaccggccga tagactc
//
LOCUS       DASWAV010000349         4125 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U2665
            k141_140403, whole genome shotgun sequence.
ACCESSION   DASWAV010000349 DASWAV010000000
VERSION     DASWAV010000349.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960316
            Sequence Read Archive: SRR10260007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 4125)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4125)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:47:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,929
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4125
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_140403"
                     /isolate="SMAG_U2665"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.36444 N 132.31889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1361)
                     /locus_tag="VF958_07270"
     CDS             complement(<1..1361)
                     /locus_tag="VF958_07270"
                     /inference="COORDINATES: protein motif:HMM:NF014713.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcdA"
                     /protein_id="HEX9148947.1"
                     /translation="MKWLGAALLAVGTAARLAAQAPDPASLLKVQPVVETPAAGRALL
                     RLDCSLASGWHVNSHKPSEDYLIATQATVDPAKGIRVGDARYPDGVMKKFAFSATPLS
                     VYEGSFKIEIPLEVEGPLPALSGRVEFQACNDTRCLAPASVPFQAETGARIAAVATSS
                     NAVPLAAAPKGGTSAAGASRDFGDMLERHGMLLVLLSIFAGGLALNLTPCVYPVIPLT
                     VGYFGRQSSGSRGRLFGLAGVYVLGMATMYSILGVAAALSGRLFGSVLQNPWVLSAIA
                     ATLVAMALSMFGAWEIRMPTSLMNRAGARSGAAGAFGMGLFVGVVAAPCIGGFIVGLL
                     AFVAARQDPFLGFLFFFTLSIGLGLPYLFLAAYSGRLARLPRAGEWMEGVKKIFGWVL
                     LAMAAYFLRSVIPPPYGKWLLPAVLVIGAVAILVTRLGLRWPVKAGAAAVLVGIAIFF
                     VPH"
     gene            complement(1358..2290)
                     /locus_tag="VF958_07275"
     CDS             complement(1358..2290)
                     /locus_tag="VF958_07275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9148948.1"
                     /translation="MISALPLILIAALGLPATGPASPSSAKPKPAPTAKAPETPAPAM
                     SVPPGPGNVIWATSTKEAHDRAEREGKFVFFEFTKQECGNCQRMDSLLYPSFDLEALL
                     VSSIPIKIDLEAPEGKAIAAIYSIEEAPAVLITTTQGRLVFLMQGFMNQGEFFRHAIG
                     AIEGYRKWAKEIAAQNVAKLSAKEAFESGNELYHRADPVAALPRLRRAVAAPGLGQKE
                     REDTLELIAAVELDLQQPAASRKTIAELLATTKDRQRRERAELFRAQIPLAENKPVEA
                     LALFRQFLKDHPTSPHTERVNQLVQQLQERVGKP"
     gene            complement(2367..2442)
                     /locus_tag="VF958_07280"
     tRNA            complement(2367..2442)
                     /locus_tag="VF958_07280"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2407..2409),aa:Phe,seq:gaa)
     gene            2486..3001
                     /locus_tag="VF958_07285"
     CDS             2486..3001
                     /locus_tag="VF958_07285"
                     /inference="COORDINATES: protein motif:HMM:NF001159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HEX9148949.1"
                     /translation="MILKVAKLGVEELRVRSREVDPKAVSRGDYEAFLADLEHTMREY
                     DGTGIAAPQVFTPLRIFLYEVNPEKRKRKEKSVPLTALFNASYEPVGAEKEEDSEGCL
                     SVPFLWGGVVPRWETIRVNALDRSGKRVAFEASGFHARVLQHEIDHLDGLVYLDRMSD
                     MKSLAYTVKFG"
     gene            3157..3756
                     /gene="nuoB"
                     /locus_tag="VF958_07290"
     CDS             3157..3756
                     /gene="nuoB"
                     /locus_tag="VF958_07290"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002769591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoB"
                     /protein_id="HEX9148950.1"
                     /translation="MPLFGKGKSLPTREPLAAGGGADFFTTRLETMVAWARSNSLWPL
                     PFATACCGIEFMSVVSSHYDLARFGAEVVRFSPRQADLLIVAGTVVDKLGPTLRKIYD
                     QMPEPKWVISMGVCASSGGFYRAYHVTQGVDEIVPVDVYVPGCPPTPEELMFGILELQ
                     RKIRSGEKSRDSRSAAGGEGSRFVFSPGERTPPPGKKTA"
     gene            3753..>4125
                     /locus_tag="VF958_07295"
     CDS             3753..>4125
                     /locus_tag="VF958_07295"
                     /inference="COORDINATES: protein motif:HMM:NF012549.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C"
                     /protein_id="HEX9148951.1"
                     /translation="MSTVEARPTLSPRAERVRERFARTPLATKVNVVDLDLPTVEVAP
                     ADWPAVARFLRDDPDCRYDLFLDLAGVDNAKRSGRPTRFEAVVHLHSLSRNEHVRVKV
                     LLPDGTEPSLPSVIDVWPAANW"
BASE COUNT          691 a         1389 c         1411 g          634 t
ORIGIN      
        1 tcgtgcggga cgaagaagat cgcgatgccg acgagaactg ccgcggcgcc ggccttgacg
       61 ggccaccgga gcccgagccg cgtcacgagg atcgcgacgg cgccgatcac cagcaccgcg
      121 gggaggagcc acttgccgta cggcggggga atcacgcttc gcaggaagta ggccgccatc
      181 gcgagcagca cccacccgaa aatcttcttc accccttcca tccattcgcc ggcgcgcggc
      241 agccgggcga gccgcccgga gtaggcggcg aggaagaggt agggaagccc gagcccgatc
      301 gacagcgtga agaagaagag aaaaccgagg aagggatcct gccgcgccgc gacgaatgcg
      361 agcaacccga cgatgaagcc gccgatgcag ggggcggcca cgacgcccac gaagagaccc
      421 atgccgaagg ccccggcggc tcccgagcgc gctccggccc ggttcatgag ggacgtcggc
      481 atgcggatct cccaggcgcc gaacatcgag agggccatcg cgacgagcgt cgccgcgatc
      541 gcggagagaa cccacgggtt ctggagcacc gatccgaaga ggcgacccga cagtgccgcg
      601 gcgactccga ggatcgagta catcgtcgcc atcccgagga catagacccc ggcgagaccg
      661 aagaggcgtc cgcgggagcc cgacgactgc cggccgaaat agccgacggt caaggggatg
      721 accggataga cgcagggcgt gaggttcaat gcgagaccgc ccgcgaagat cgagagcaga
      781 acgagcagca tgccgtggcg ctcgagcatg tcgccgaagt ccctggaggc gccggcagca
      841 gacgtcccgc ccttcggcgc cgcggcgagg gggacggcgt tactcgaggt cgcgactgca
      901 gcgatgcggg cgcccgtctc cgcctggaac gggaccgacg ccggcgcgag gcagcgggtg
      961 tcgttgcagg cctggaactc cacccggccg gagagcgccg gaagcgggcc ctcgacctcg
     1021 agcgggatct cgatcttgaa agagccctcg tagaccgaga ggggagttgc tgagaacgcg
     1081 aacttcttca tgacgccgtc ggggtaccgc gcgtcgccga cgcgaatgcc ctttgctgga
     1141 tcaacggtcg cctgcgtggc gatcaggtag tcctccgacg gcttgtggct gttgacgtgc
     1201 cacccggagg cgagagagca atcgagtcgc aggagcgcgc gacccgccgc gggcgtctcc
     1261 accaccggct ggaccttcaa gaggctcgcc gggtcgggag cctgggccgc gagcctcgcg
     1321 gccgtcccca cagcgaggag cgccgcgccg agccacttca tggcttcccg acgcgctcct
     1381 gcagctgctg cacgagctgg ttcactcgct cggtgtgcgg cgaagtcggg tgatctttca
     1441 ggaactggcg gaagagggcg agcgcctcga caggcttgtt ctcggccagc gggatctggg
     1501 cgcggaagag ctccgctcgt tcgcgccgct gccggtcctt cgtcgtcgcg agcagctcgg
     1561 cgatcgtctt tcgcgaagcg gccggctgct ggagatcgag ctcgacggcc gcgatcagct
     1621 cgagggtgtc ctcgcgctcc ttctggccca gtcccggcgc ggccacggca cgccgcaggc
     1681 gcgggagcgc cgccaccgga tcggcgcgat ggtacagttc gttgcccgac tcgaaagctt
     1741 ctttcgccga cagcttcgcg acgttctgcg ccgcgatctc cttcgcccac ttgcggtatc
     1801 cctcgatggc tccgatggcg tgccggaaga actcgccctg gttcatgaag ccctgcatga
     1861 ggaagacgag ccgcccctgc gtcgtcgtga tgagcacggc cggggcctcc tcgatcgaat
     1921 agatcgccgc aatcgccttg ccctcgggtg cctcgaggtc gatcttgatc gggatcgagg
     1981 agacgaggag tgcctcgagg tcgaaggagg ggtagaggag cgaatccatg cgctggcagt
     2041 tgccgcactc ctgcttcgtg aattcgaaga acacgaactt gccctcgcgc tccgcgcgat
     2101 cgtgcgcttc cttggtgctc gtcgcccaga tgacgtttcc gggcccgggc gggaccgaca
     2161 tggcgggcgc gggcgtctcc ggcgctttcg ccgtgggcgc cggcttgggc ttcgcggacg
     2221 agggagaagc gggcccggtc gccggcaggc ccagggcagc gatgaggatc agcggaagag
     2281 cagaaatcaa tcgaacctcc cgaatgtggt gctgcctccg aaacgggacc ggccgggcgt
     2341 gaattccccc ggaggcgcgg agaacgtggt gcccagggac ggaattgaac cgccgacaca
     2401 gggattttca gtcccttgct ctaccaactg agctacctgg gcgtagccgg tcgggccgcg
     2461 cagtgtagca ctaagattgc ccgccgtgat cctgaaagtc gcgaagctcg gcgtcgaaga
     2521 gcttcgggtg cgctcgcgcg aagtcgaccc gaaagccgtg tcgcgggggg actacgaagc
     2581 cttcctcgcg gacctcgagc acaccatgcg cgagtacgac gggacgggga tcgcggcgcc
     2641 gcaggtcttc acgccgctcc ggatcttcct ctacgaggtc aaccccgaga agcgcaagcg
     2701 caaggagaaa agcgtcccgt tgacggcgct cttcaacgcg agctacgagc cggtcggcgc
     2761 ggagaaggag gaggattccg agggctgcct ctccgtgccc ttcctctggg gcggcgtcgt
     2821 gccgcgctgg gagacgatcc gcgtcaacgc cctcgaccgc tccggcaagc gagtcgcgtt
     2881 cgaggcgtcg gggtttcacg cccgcgtcct gcagcacgag atcgatcacc ttgacggact
     2941 cgtgtatctc gaccgcatgt ccgacatgaa gtcactggcc tatacggtca agttcggctg
     3001 aagcagggca ccgctcgggc ggtcgagtgc cgcccctcac ccccggcccc ctctccccgc
     3061 acgcggggcg agggggcaac cttcggtggt cgagtcgtgt ccactccttg tgaaaacttt
     3121 cacaatccgc gatccctgtg acagaatcct cccgccatgc ctctcttcgg caagggcaag
     3181 tcgctgccca cccgggaacc tctggcggcg ggcggcggtg ccgacttctt cacgacgcgc
     3241 ctggagacga tggtcgcctg ggcgcgctcg aactcgctgt ggccgctgcc gttcgcgacg
     3301 gcgtgctgcg gcatcgagtt catgtccgtc gtctcgtcgc actatgacct cgcccgcttc
     3361 ggcgccgagg tcgtgcgttt ctctccgcgg caggcggacc tcctgatcgt cgccggcacc
     3421 gtcgtcgaca agctcggtcc gacgctgcgc aagatctatg accagatgcc cgagcccaag
     3481 tgggtgattt cgatgggcgt ctgcgcgtcc tcgggcggtt tctaccgcgc gtaccacgtc
     3541 acgcagggag tcgacgaaat cgtccccgtg gacgtgtacg tccccggctg ccccccgacg
     3601 cccgaggagc tcatgttcgg gatcctcgag ctccagcgga agatccgatc cggggagaag
     3661 tcccgggaca gccggtccgc cgccgggggc gaggggagcc gattcgtctt ctctccgggc
     3721 gagaggactc cgcctccggg gaaaaaaacc gcttgagcac ggtcgaggcg cggccgaccc
     3781 tctcgccgag agcggagcga gtccgcgagc gcttcgcgcg cactccactc gcgacgaaag
     3841 tcaacgtcgt cgatctcgat cttccgacgg tcgaggtggc gcccgcggac tggcccgccg
     3901 tcgcccgctt cctgcgcgat gacccggact gccgttacga tctcttcctc gacctcgccg
     3961 gcgtggacaa cgcgaagcgt tcgggccgtc cgacgcggtt cgaggccgtg gtccatctcc
     4021 attcgctctc ccgcaacgag cacgtccgcg tcaaggtgct cctgcccgac ggaaccgaac
     4081 cgtcgctccc ttctgtcatc gacgtctggc ccgcggcgaa ctggt
//
LOCUS       DASWAV010000350         2180 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U2665
            k141_98675, whole genome shotgun sequence.
ACCESSION   DASWAV010000350 DASWAV010000000
VERSION     DASWAV010000350.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960316
            Sequence Read Archive: SRR10260007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2180)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2180)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:47:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,929
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2180
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_98675"
                     /isolate="SMAG_U2665"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.36444 N 132.31889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            207..887
                     /locus_tag="VF958_07300"
     CDS             207..887
                     /locus_tag="VF958_07300"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEX9148952.1"
                     /translation="MEGIFRPVALVPAYQAEGTVGEVVNGLLRHVTRVVVVDDGSTDA
                     TSSEAERAGAEVIRLSDNGGKGSALRAGLARILATDATHVAFVDADGQHDPDDLPPLL
                     DAARGGDTFVVGSRMSGEDGIPAVRFRTNEIGSRILTRMTGLEVEDGQSGYRVIAAPV
                     LRRLSLSSKGYAIETEILLKAAAHVPRLRNVPVRAIYSSARSHYKPFRDTWIISWGAV
                     YYKVFEVD"
     gene            889..1764
                     /locus_tag="VF958_07305"
     CDS             889..1764
                     /locus_tag="VF958_07305"
                     /inference="COORDINATES: protein motif:HMM:NF023385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3473 domain-containing protein"
                     /protein_id="HEX9148953.1"
                     /translation="MSSFEITLLRTPGKAPRVLTIDAEDWFHVCGEDFYSDPRRWDSF
                     PSRIETTFLRLLDVLENGGHRATVFFLGWIARRKPHLVSEAVARGHEAAVHGDSHRRA
                     DEMTADEFRSDLRNARDSIEDAGGVACTAYRAAEWSIRHPGDRALSILAEEGFRCDAS
                     ATSVPPLGHAENPLGPYRISRNGTAILEFPPLTGRGFGRRLPVGGGWPFRMLPEKRIV
                     ESERAYRDRGFPAVFTFHPWEFDPDHPPMDELPPLLSAVHFAMLRRLPGRFDRWLARD
                     RCVALRDVISSLERA"
     gene            1761..>2180
                     /locus_tag="VF958_07310"
     CDS             1761..>2180
                     /locus_tag="VF958_07310"
                     /inference="COORDINATES: protein motif:HMM:NF012901.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase domain-containing protein"
                     /protein_id="HEX9148954.1"
                     /translation="MNEPGFLFSGQLAEAVGMGRDFWEADPEARDLFARTSARCGRDL
                     EALLFEGPAEALHENLVAQAGVYLVSTLAARALERHGIRPAATAGYSLGNYAAMVASQ
                     AISYDDALEVLVAVERETERLGIRGSMGAVVGTRRETV"
BASE COUNT          330 a          737 c          742 g          371 t
ORIGIN      
        1 ccgaggaccg cggacagcag gatcatccca ccggcgacgg cgatcgcgaa gagcatcagg
       61 atgaggatcg gaaggtagct ttcggccatc cgcacctcgc ctcgttcaga cctcgcgcgg
      121 cgagctttcg gagggagccg cgccggttgt gaactttttc acaagcgcgc ggaacgagtc
      181 aacgagaacg acgttacgat cggagcgtgg aggggatttt tcggccggtc gcgctggtac
      241 cggcctatca agcggaggga accgtcggcg aggtcgtcaa cggcctgttg cgccacgtca
      301 cccgtgtcgt cgtggtcgac gacggctcga ccgacgcgac ctcgagcgag gcggaacggg
      361 ccggcgcgga agtcatccgt ctctccgaca acggcgggaa aggaagcgct ctgcgggcgg
      421 gcctggcgag gatcctcgcg accgatgcga cccacgtcgc cttcgtcgat gccgacggcc
      481 agcacgaccc ggacgacctg ccccctctgc tcgacgcggc gcgcggcggc gacacgttcg
      541 tcgtcggctc gcggatgagc ggcgaggacg gaatcccggc cgtccggttc cgcacgaacg
      601 agatcggcag ccgcatcctg acgcggatga ccggtctcga ggtcgaggac gggcagtcgg
      661 gatatcgggt catcgccgcc cccgtcctgc ggcggctgtc gctttcgtcg aagggatacg
      721 cgatcgagac ggagatcctc ctgaaggcgg cggcacacgt cccgcgcctc cgcaacgtgc
      781 cggtgcgagc gatctattcc tcggcgcggt cgcactacaa gccgtttcgc gacacgtgga
      841 tcatttcgtg gggagcggtg tactacaagg tcttcgaagt cgattgacgt gagttccttc
      901 gagatcaccc tgcttcggac tcccggcaag gccccgcgcg tgctcacgat cgacgccgag
      961 gattggttcc acgtgtgtgg agaggacttc tactccgatc cgcgtcgctg ggactccttt
     1021 ccttcgcgca tcgagacgac gtttctgcgg ctgctcgacg ttctcgagaa cggaggccac
     1081 cgcgccacgg tcttcttcct gggctggatc gcccggcgca agccccacct cgtttccgaa
     1141 gcagtcgctc ggggccacga ggccgcggtc cacggcgatt ctcaccggcg cgccgacgag
     1201 atgacggcgg acgagttccg aagcgacctg cggaatgctc gcgactcgat cgaggacgcc
     1261 gggggagttg cctgcaccgc ctaccgcgcg gccgagtggt cgatccggca ccccggcgat
     1321 cgcgcgctct cgatcctcgc ggaagaggga tttcgctgcg acgcatcggc gacgtctgtt
     1381 ccgccgctgg ggcacgcgga gaacccgctc ggtccctatc ggatctcgag gaacggaacc
     1441 gccatcctcg agtttccccc gctgaccggc cgcggcttcg ggaggcgcct tccggtcgga
     1501 ggcgggtggc cgttccggat gcttcccgag aagcgaatcg tcgagtccga gcgcgcctac
     1561 cgcgaccgcg gttttccggc agtcttcacg ttccacccgt gggagttcga tcccgatcac
     1621 ccgccgatgg acgagctgcc tccgctgctc tccgcggtgc acttcgccat gctccgccgc
     1681 ctgcctggtc gtttcgatcg gtggctcgcg cgggaccgct gtgttgcact gcgcgatgtg
     1741 atctcgtcgc tcgagcgcgc atgaacgaac cgggatttct cttctcgggc cagctcgccg
     1801 aggccgtggg gatgggccgc gatttctggg aagccgaccc ggaagcgagg gacctcttcg
     1861 cgcggacttc cgcccggtgc gggcgcgacc tcgaggcgct ccttttcgag ggtccggccg
     1921 aagcccttca cgagaacctc gtcgcgcagg ccggtgtgta tctcgtctcg acgctcgccg
     1981 cgcgggcgct cgagcggcac gggatccgtc cggcggcgac cgccggttac agcctcggta
     2041 actacgccgc gatggtggct tctcaggcga tctcctacga cgacgcgctc gaagtactcg
     2101 tcgcagtcga gcgcgaaacc gaacgcctcg gcatccgcgg atcgatgggc gcggtcgtgg
     2161 gcacccggcg ggagaccgtc
//
LOCUS       DASWAV010000351         2362 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U2665
            k141_777656, whole genome shotgun sequence.
ACCESSION   DASWAV010000351 DASWAV010000000
VERSION     DASWAV010000351.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960316
            Sequence Read Archive: SRR10260007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2362)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2362)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:47:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,929
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2362
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_777656"
                     /isolate="SMAG_U2665"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.36444 N 132.31889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2362)
                     /locus_tag="VF958_07315"
     CDS             complement(<1..>2362)
                     /locus_tag="VF958_07315"
                     /inference="COORDINATES: protein motif:HMM:NF024589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEX9148955.1"
                     /translation="QLCRDLLRRDPGARPSGEGISKRLGEARSETLDDTASITIHAAA
                     PFVGRESELRSLRDAFSATQEGRAVEVHVHGTSGIGKTALVRHFLEELRWKETAIVLT
                     GRCYERESVPFKALDSLIDALSRHLKGLTRAEVDALLPRDVPALIRLFPVLRRVEAVA
                     TARRRALEIPDSQELRRRGFAAVRELLGRLASQKPVVLFIDDLHWGDVDSALLLAEVL
                     RPPDAPAVLLILAYRTEEVETSPLLRKLLTERDRPPDVREIPVRELPLHEAQELAYAL
                     LSDGAPSSREQADAIARESAGSPFFIDELARYTRAGADLLGAPKRLETTTVDSVIHAR
                     ISHLPEDARRALQLVAVAGQPIEVAVLREAASPDIGPHAIGILRAGRLIRARHTVDRE
                     EIEPYHDRIRQTVLAHVTAEALREDHLRLALAWEASRRADPETLMVHFQEAGEIEKAA
                     EYAATAAARASDALAFDRAARLYSLAFELGSNRDPEARRALRALQGRALANAGRGAEA
                     AKAYLGAIEGAPAAEALELQRRAAEQLLRSGHIQEGMPVLRSLLAKLGIEIAETPRQA
                     LVSLLLRRARVAMRGLWFRERDETQISAEDLLRIDTCWSIVTGYALADIIRSADIQAR
                     HFLLALEAGEPYRLALAFAGEAALKATEGQKTRKRVDRLLEVGRALARRVDRPHALAM
                     IEFASGIAAYMQGRWKEGWIRGEEAERIFRERCTGVTWELDLTHIYSLRALFYLGRIS
                     QLSARIPVLIREALERDDLFAATNLRTRHAYVAWLAADDPNKARAEL"
BASE COUNT          380 a          785 c          812 g          385 t
ORIGIN      
        1 agctcggccc gtgccttgtt cgggtcgtcg gcggcgagcc aggcgacgta agcgtgccga
       61 gtccgaagat tcgtggcggc gaacaggtcg tcccgctcga gagcttcccg gatgagcacc
      121 ggaatgcggg cggagagctg cgagatccgg ccgagataga aaagggcgcg caacgagtag
      181 atgtgtgtta aatcgagctc ccacgtcaca cccgtacagc gctcccggaa gatccgttcc
      241 gcctcctctc cccgaatcca cccctctttc catcgtccct gcatgtaggc tgcgatgccg
      301 gaagcgaact cgatcatcgc gagcgcgtgc gggcgatcga cgcggcgcgc gagcgcgcgg
      361 ccgacttcga ggagacggtc gacccgcttc cgtgttttct ggccctccgt cgccttcagc
      421 gcggcctcgc ctgcgaacgc gagcgccaac cggtacggct ctcctgcctc cagggccagc
      481 aggaaatggc gggcctggat gtccgccgat cggatgatgt cggcaagcgc gtacccagtc
      541 acgatcgacc agcaggtatc gatccgcaga agatcttcgg cggagatctg cgtttcgtcg
      601 cgttcgcgga accacagccc gcgcatggcc acgcgcgctc gcctcagcag gagggaaacc
      661 agagcctgac gcggagtttc cgcgatctcg atcccgagct tcgcgagaag ggagcgcaga
      721 accggcatcc cctcctggat gtgaccgctt cgaaggagtt gttcggcggc ccgcctctgc
      781 agttcgagag cctcggccgc gggagctcct tcgattgcgc ccaggtaggc cttcgccgct
      841 tctgcacctc gtcctgcgtt cgcgagcgct cgcccctgaa gcgcccgaag agctcttcgg
      901 gcctcggggt cccgattgga gccaagctcg aacgcgagcg aatagaggcg cgccgcgcgg
      961 tcgaacgcta gagcgtccga cgcccgcgcg gccgcggtgg cggcgtattc cgcggccttc
     1021 tcgatctcgc ccgcctcctg gaagtggacc atcaaagtct cggggtccgc ccggcgggac
     1081 gcctcccacg cgagggcgag ccggagatgg tcttcgcgga gcgcctctgc cgtgacgtgc
     1141 gcgagcacgg tctgacggat ccggtcgtgg tagggctcga tctcttcgcg gtcgacggtg
     1201 tgtctcgcgc gaatcagacg gcccgctcgg aggataccga tggcgtgagg gccgatgtcg
     1261 ggagaagcgg cttcccggag aacggcgacc tcgatcggct gacccgccac ggccacgagc
     1321 tggagggctc gccgggcgtc ctccggcaga tgcgaaatgc gggcgtgaat cacgctgtcg
     1381 acggtcgtcg tttcgagtcg cttcggggcc cccagcaggt cggccccggc acgtgtatag
     1441 cgcgcgagct cgtcgatgaa gaaagggctt ccggcggatt cgcgcgcaat ggcgtctgcc
     1501 tgctctcgag aactcggcgc tccatccgag aggagggcat aggcgagctc ctgcgcttcg
     1561 tgaagaggca gctcgcgcac cggaatctct cggacgtcgg gaggccggtc gcgctcggtc
     1621 agaagttttc ggaggagagg gctggtctcg acctcctcgg tccggtacgc gaggatgagc
     1681 aggaccgctg gcgcgtcggg aggccggagc acctcggcga ggagcagggc gctgtcgacg
     1741 tctccccagt ggagatcgtc gatgaacaac acgaccggct tctggcttgc cagccggccg
     1801 agaagctcgc ggaccgccgc aaatccgcgg cgccggagct cctgcgaatc cgggatctcg
     1861 agcgcccggc gtcgggccgt cgccaccgcc tcgacccggc gcagcaccgg aaacagtcga
     1921 atgagagcgg gcacgtcgcg cggcaggagc gcatcaacct ctgcccgtgt gaggcccttc
     1981 aggtgccggc tcagtgcatc gatcaggctg tcgagtgctt tgaaaggaac ggactcgcgc
     2041 tcgtagcagc gtcccgtcaa aacgatcgcg gtctccttcc atcgcagctc ttcgaggaaa
     2101 tgccggacga gggccgtctt tccgatcccc gaagttccgt ggacgtggac ctcgaccgcg
     2161 cggccctcct gcgtcgcgga aaaggcgtct cggagcgacc ggagttcgct ttcgcgtccg
     2221 acgaatgggg cggcggcgtg aatcgtgatc gaggccgtat cgtccagggt ttccgaccgg
     2281 gcctcgccca ggcgcttcga gatcccctct cccgacgggc gggctcccgg atcccggcgc
     2341 agaagatccc ggcagagctg gt
//
LOCUS       DASWAV010000352         2394 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U2665
            k141_539274, whole genome shotgun sequence.
ACCESSION   DASWAV010000352 DASWAV010000000
VERSION     DASWAV010000352.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960316
            Sequence Read Archive: SRR10260007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2394)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2394)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:47:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,929
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2394
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_539274"
                     /isolate="SMAG_U2665"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.36444 N 132.31889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(180..449)
                     /gene="rpsS"
                     /locus_tag="VF958_07320"
                     /pseudo
     CDS             complement(180..449)
                     /gene="rpsS"
                     /locus_tag="VF958_07320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002974021.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
     gene            complement(454..1284)
                     /gene="rplB"
                     /locus_tag="VF958_07325"
     CDS             complement(454..1284)
                     /gene="rplB"
                     /locus_tag="VF958_07325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020676894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HEX9148956.1"
                     /translation="MPVKKFNPTSAGRRFQTSPTFEEVSATRPEKSLTVGLRSTGGRN
                     SQGRITSRYMGGGHKRLYRIIDFRRDKMGVPAKVATIEYDPNRTARIALLHYADGEKR
                     YILAPNGLEVGAQVVAGPAADILPGNALPLRGIPLGTTIHNLELKRGRGGQLVRSAGA
                     AAQLMAKEGSYAQVRLPSGEVREIHIECYATIGQVGNIEHENVSIGKAGRNRWIGRRP
                     HVRGVAMNPVDHPMGGGEGKTSGGRHPTSPWGWKTKGMKTRNNKRTDSFIVRRRKAKA
                     "
     gene            complement(1287..1577)
                     /locus_tag="VF958_07330"
     CDS             complement(1287..1577)
                     /locus_tag="VF958_07330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012611041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HEX9148957.1"
                     /translation="MDPQRVIRRPLITEKATRLKEATNTVCFEVARDANKIEIARAVQ
                     TLFGVKVADVRVANREGKWKRMGKFIGQRKGWKKAYVRLAAGEKPIEFFEGV"
     gene            complement(1577..2206)
                     /gene="rplD"
                     /locus_tag="VF958_07335"
     CDS             complement(1577..2206)
                     /gene="rplD"
                     /locus_tag="VF958_07335"
                     /inference="COORDINATES: protein motif:HMM:TIGR03953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HEX9148958.1"
                     /translation="MATLPVVNWKKERVGEVNLPAAVFEYPFRSHLVWEAVKAYQAGQ
                     RQGTHKTKTRAEVSGSGRKPFKQKGTGRARQGGSRPPIHRHGGTAHGPTPRSYGQNLS
                     VGEKKNALKAALSRRVKEERLLVLEGFDLESHRTKDLKAAVEGLGVSGKALFVDSRGN
                     ENFARASANVSQWKLVDPLAVNVYDVMSHGTVVVSRDAFSRVVATLGGE"
     gene            complement(2212..>2394)
                     /locus_tag="VF958_07340"
     CDS             complement(2212..>2394)
                     /locus_tag="VF958_07340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HEX9148959.1"
                     /translation="SRVIKGTRSSGRLGGRQATAKNLIVVRVDTEKNLIYLRGPVPGA
                     RRSLVKIVRSTFAKPE"
BASE COUNT          320 a          841 c          761 g          472 t
ORIGIN      
        1 cttcttgccg cgcacgaggt ccgccaccag gcggaccttc tgcgcggaac ccttcatgta
       61 ccggagcctg gccgttgctt tcatggcttc cctcaggcct tcggcgccgc cgcggccggg
      121 gccgcgcccg gagccgagac cggcttgccg gcgccggcgg cagcgacggc cgctccggcc
      181 tccgccgcct tcttgccgcc gtggcccttg aagattcgcg tcggagcgaa ctcgccgagc
      241 ttgtggccga ccatgttctc cgcgacgtag accggaatga acttgcgccc gttgtgcacg
      301 gcgatcgtgt gcccgaccat gtccgggacg atggtcgagc gccgcgacca cgtcttgatc
      361 actttccgct cgcgggtgga attcaactcc gccaccttct tgacgaggtg gtcgtcgatg
      421 aacgcgccct tcttgatcga tcgcgccatt tttctacgcc ttcgccttgc ggcggcggac
      481 gatgaacgaa tccgtccgct tgttgtttct ggtcttcatt cccttcgtct tccagcccca
      541 cggcgacgtg gggtggcgcc cgccggaggt cttgccttcg ccgccgccca tcggatggtc
      601 gaccgggttc atcgcgacgc cgcgcacgtg gggacggcga ccgatccagc gattgcgtcc
      661 ggccttgccg atcgacacgt tctcgtgctc gatgtttccg acctggccga tggtcgcgta
      721 acactcgatg tggatctcgc ggacctcccc ggagggaaga cggacctggg cgtagctccc
      781 ctctttcgcc atgagctgcg cggcggcgcc ggcggatcgg acgagctggc cgccgcggcc
      841 ccgcttcaac tccaggttgt ggatcgtcgt gccgagcggg atccctctca gagggagcgc
      901 gttccccggc aggatgtcgg ccgccggtcc ggcgaccacc tgcgcgccga cctcgagtcc
      961 gttcggcgcg aggatgtagc gcttctctcc gtcggcgtag tggagcagcg cgatccgggc
     1021 ggtgcggttc gggtcgtact cgatcgtggc gaccttcgcc ggcacgccca tcttgtcccg
     1081 tcggaagtcg atgatgcggt agagacgctt gtggccccct cccatgtagc gggaggtgat
     1141 ccggccctgg ctgttcctgc cgccggtcga acgcagcccg acggtcagcg acttctccgg
     1201 acgcgtcgcg gagacctctt cgaacgtggg cgacgtctgg aaacggcgcc ccgccgaagt
     1261 cgggttgaac ttcttcacgg gcatttctac accccttcga agaactcgat cggcttctcg
     1321 cccgcggcga gacgcacgta ggccttcttc cagcccttcc gctgaccgat gaacttcccc
     1381 atccgcttcc acttgccttc ccggtttgcg acccggacgt ccgcgacctt cactccgaag
     1441 agcgtctgca cggcgcgggc gatctcgatc ttgttcgcat cccgggcgac ctcgaagcac
     1501 acggtgttcg tcgcctcttt cagacgcgtg gccttttcgg tgatgagcgg tcggcgaatg
     1561 acgcgctgcg ggtccatcac tcgcctccga gcgtcgcgac gacgcgggaa aacgcgtccc
     1621 tcgacacgac gacggttccg tgactcatca cgtcgtacac gttgacggcg agcggatcca
     1681 cgagcttcca ctgcgagacg ttcgccgatg cgcgtgcgaa gttctcgttg ccgcgggagt
     1741 cgacgaagag cgccttcccc gagactccga gtccctccac cgcggccttc agatctttcg
     1801 tgcgatggct ctcgaggtcg aaaccctcga gcacaagcag cctctcctct ttcacccgcc
     1861 gcgacagggc cgccttcagc gcgttcttct tctcgccgac cgagagattc tgtccgtacg
     1921 agcgcggcgt cggcccgtgg gcggtgccgc cgtggcggtg gatcggcggt cgtgagccgc
     1981 cctgccgggc ccggccggtc cctttctgct tgaaaggctt gcggccgctg ccggagacct
     2041 cggcacgggt cttggtcttg tgagttccct gccgctgacc ggcctggtac gccttgaccg
     2101 cttcccagac gaggtggctg cggaagggat actcgaacac cgcggcggga aggttgacct
     2161 cccccacgcg ctccttcttc cagttcacga cgggcagtgt ggccatgtcc atcactccgg
     2221 cttcgcgaat gtcgagcgca cgatcttgac gagcgagcgc cgcgcgcccg gtaccgggcc
     2281 gcgcaggtag atcaggttct tttcggtgtc gactctcacg acgatgaggt tcttggccgt
     2341 cgcctgccgc ccgccgagcc ggccggagga tcgcgtcccc ttgatcacgc ggga
//
LOCUS       DASWAV010000353         1822 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U2665
            k141_179748, whole genome shotgun sequence.
ACCESSION   DASWAV010000353 DASWAV010000000
VERSION     DASWAV010000353.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960316
            Sequence Read Archive: SRR10260007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 1822)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1822)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:47:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,929
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1822
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_179748"
                     /isolate="SMAG_U2665"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.36444 N 132.31889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..810)
                     /locus_tag="VF958_07345"
     CDS             complement(<1..810)
                     /locus_tag="VF958_07345"
                     /inference="COORDINATES: protein motif:HMM:NF015368.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9148960.1"
                     /translation="MKGFLRGTLLALLSGIAFALAGCGGGGGDSNPGGASAPTVVVQP
                     LPLPGPYAVACSNIVQDFSRVGAGVEVQSYWEGKPWDNGTTRYVTDLLADPGNTLIAT
                     VTAPNDSDLYGLFAGQNVEFVVLVCYPTTVANPYDNYSLPTGKVVPHMLTGSDPPLFA
                     DNSARYPVLVFSHGYGGSPLSSDYISALSVFASYGYVVIAPFHGDVRFSDLKIDNLAD
                     AIALASHLDNFTALQALRPLAISKALDLVLAHPQWQGHIDNTRIGGFGASMG"
     gene            complement(1138..>1822)
                     /locus_tag="VF958_07350"
     CDS             complement(1138..>1822)
                     /locus_tag="VF958_07350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012529461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEX9148961.1"
                     /translation="PPDARARDELEGALKNLTLVNCYLGNGRAVLKHLAAMNAGTAGE
                     GFTVLDVATGAADIPVTIAMWARQAGIRVGITAVDLDPISIGFARQRCESHPEITLAV
                     ADGFALPFANQRFDYVLCSKTVHHFTDEEALKMIKEFSRVARRGYIIVDLRRSWIAYF
                     LIYLLTRLFTRNRLTRSDGPLSVLRSYTPGELAALASRSGTSTFTVSREPFWLLVLAG
                     RSAPAEAAT"
BASE COUNT          354 a          585 c          552 g          331 t
ORIGIN      
        1 gcccatgctc gccccgaagc cgccgatcct tgtattatcg atgtggcctt gccattgcgg
       61 atgcgcgagc acgaggtcga gcgccttgga gatcgcaagc ggtcgcagcg cctgcagcgc
      121 cgtgaagttg tccaggtgcg acgccaaggc aatggcgtcg gcgaggttgt cgatcttgag
      181 gtcggaaaag cgcacgtcgc cgtgaaacgg ggcgatgaca acgtaaccgt aactcgcgaa
      241 aacggacagc gccgaaatgt agtcgctgga gagcgggctg ccgccgtagc catgcgaaaa
      301 cacgagcacc ggataacgag ccgaattgtc cgcaaatagc ggcggatccg atccggtcag
      361 catgtgcggc acgacctttc cggtcggcag cgaatagttg tcataggggt tggcgaccgt
      421 cgtggggtag cacacgagca ccacgaactc gacattctgt ccggcaaaca aaccgtagag
      481 atccgagtcg ttcggcgcgg tcaccgtggc gatcagcgtg ttccccgggt ccgccagcag
      541 atcggtcaca taacgcgtcg tgccgttgtc ccacggtttg ccttcccaat agctctgcac
      601 ctccacaccg gcacccacgc gactgaaatc ctggacgatg ttgctgcacg caaccgcgta
      661 gggacccggc aacggcaaag gctgcacgac cacggtcggc gccgaggcgc cccccgggtt
      721 gctgtcgccg ccaccgccgc cacatccggc gagtgcgaat gcgatccccg acaagagcgc
      781 caacagcgtt ccccgcaaga atcccttcat gactggatct cctcggccgc aacctcttcg
      841 ggaaaattgc cggccatgcc gcttgccaca ttccacggcg tcattgacgc gccaggaaaa
      901 tcaattgctg ttgccgtcat ccaatcgatg gaccttgccc gctcgcatcg tttcctcatg
      961 ttttcctcgt ccttcaaccg ggaggcgcgc gcattcccgg atgactcaca cggtaatcct
     1021 gcccgccagc caacggaatc ggattccgtc cgaaacgcga gtcgccgtta gtctgattct
     1081 cggcccggtg tttgtcctat ggaaaggaag gggaagtcca cttgccggcg ggatccctca
     1141 cgtcgcggct tctgccggcg ctgaccttcc cgccaggacg aggagccaga acggttctcg
     1201 ggacaccgtg aaggtcgaag tcccgctcct ggaggcaagc gcggcaagtt cgcctggggt
     1261 gtacgacctc agcaccgaga gcggcccgtc gctgcgggtc aggcggttcc tggtaaagag
     1321 ccttgtcagg agatagatca ggaagtaggc gatccagctc cgccgcaagt cgacgatgat
     1381 atatccccgc cgtgcgaccc gtgaaaactc cttgatcatc ttcaacgcct cttcatcggt
     1441 gaagtggtgc accgtcttgc tgcaaagcac gtaatcaaac cgttgattcg cgaatggcag
     1501 ggcgaacccg tccgccacgg cgagagtgat ctccggatgg gactcgcacc tctgcctggc
     1561 gaatccgatg ctgatcgggt caaggtcgac cgccgtgatg ccgacgcgga tccctgcctg
     1621 tctcgcccac atcgcgatgg tgaccgggat gtccgccgca ccggtagcca catccagcac
     1681 ggtaaatcct tcgcccgcgg tcccggcgtt catggcggcg aggtgcttca ataccgcgcg
     1741 gccattgccg aggtagcaat tcacgagggt caggtttttg agggccccct cgagttcatc
     1801 acgggcgcgg gcgtccggcg ga
//
LOCUS       DASWAV010000354         7409 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U2665
            k141_218676, whole genome shotgun sequence.
ACCESSION   DASWAV010000354 DASWAV010000000
VERSION     DASWAV010000354.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960316
            Sequence Read Archive: SRR10260007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 7409)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7409)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:47:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,929
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7409
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_218676"
                     /isolate="SMAG_U2665"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.36444 N 132.31889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..686
                     /locus_tag="VF958_07355"
     CDS             <1..686
                     /locus_tag="VF958_07355"
                     /inference="COORDINATES: protein motif:HMM:NF018652.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9148962.1"
                     /translation="GSELLLSADLILGLPGQTSTSFRRSVEAVGAAGFGHLSIYLLET
                     DKSRSLDEDRRAHPERYLSDDEQADAWIEAGEALAARGLRHYEISNWARPGDEARHNV
                     KYWTRTPTLGLGISAHELWADRRRANVSAIPQYVSALSEGRRPLALDQPIPEAEAAKD
                     RLVLGLRLARGVDAGEIEGWIAVEGDPLLPEDYRAWFEAGLLERENGRVRFSERGFLL
                     SNEVLCRFV"
     gene            complement(825..2981)
                     /locus_tag="VF958_07360"
     CDS             complement(825..2981)
                     /locus_tag="VF958_07360"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014100589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-translocating pyrophosphatase"
                     /protein_id="HEX9148963.1"
                     /translation="MSYDLALPLIMVFSIGSLFVAGYLISWVLKRDTGTPAMREISNA
                     IKEGAEAFLRRQNLTIVLLAAVLAAVIFLLYGFVRAHHDFDPVPTALKLALWTTISFA
                     LGATCSVIAGYIGMWISIRSNIRTASAARTSLNDALRIALRGGAVSGLFVVAMSLFGV
                     GGLYWLVSHLTDVPPPQIPLLIVGYGFGASFVALFAQLGGGIYTKAADVGADLVGKVE
                     AGIPEDDPRNPAVIADLVGDNVGDCAGRGADLFESTAAENIGAMILGATLAGASERAG
                     MGFAAGIVGVMLFPLVARAFGLLASIVGIFFVHTREDRDPMQALNRGYYLTALLAIGG
                     FFLATRWLLYNPAAPEAWWKFFLCGIIGVLTSVAFVYITQYYTEYKYRPVREIANASQ
                     TGPATNVIAGVAVGFECTLLPVLAISLAILTSFKIGAWALPGGGLFGTAVATMGMLGT
                     AAYILAMDTFGPITDNAGGIVEMSRQPEEIRRKTDRLDAVGNTTKALTKGYAIGSAAL
                     AAFLLFSAYLDELKNYGSPLAAVNIAKPEVFIGGLLGAMLVFFFSGLAIRAVGKAAGV
                     VIQDVRDQFKAHPGIMQGTEKPDYGRTVDIVTRGALRAMIAPGLVAVGMPIAVGLFFR
                     LFHVGAEAVAALLMVGTIAGVVLATFLNNSGGAWDNAKKFIESGALGGKGSPTHKAAV
                     VGDTVGDPFKDTAGPSLHVLIKLLATITLVLAPLFI"
     gene            complement(3066..3527)
                     /locus_tag="VF958_07365"
     CDS             complement(3066..3527)
                     /locus_tag="VF958_07365"
                     /inference="COORDINATES: protein motif:HMM:NF014525.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HEX9148964.1"
                     /translation="MAAAVGGPAAGGYKSDINITPLVDIVLVLLIIFMVITPLLQMGY
                     DVKVPPKATVENPPPSLDQLIVSLTPQGRIYLNKEQLDAQSLSLRLTEILKNRRDKTV
                     FFSADDKVTYGEVARVMDIVRTAGAKNLGIVLETVPVEGLAAAPPAPAPAQ"
     gene            complement(3558..3992)
                     /locus_tag="VF958_07370"
     CDS             complement(3558..3992)
                     /locus_tag="VF958_07370"
                     /inference="COORDINATES: protein motif:HMM:NF014525.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HEX9148965.1"
                     /translation="MGMGGGGGRHNLQSDINVTPLVDVCLVLLIIFMVVTPMLQKGIP
                     IQLPVTENPDKKPENAKQKLITVAWGDPPKYYIDTKWLPKEEFQKELDEMYQRDPAAE
                     IVIKADQRLKYGQVKEVMRITKEAGFQDVGLIVDKKQKGTAG"
     gene            complement(4008..4712)
                     /locus_tag="VF958_07375"
     CDS             complement(4008..4712)
                     /locus_tag="VF958_07375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011419327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HEX9148966.1"
                     /translation="MSFNPVDLWNTMTPLAKGVAVLLILMSIYSLTVAIERWIYFAKA
                     KKQSLAFAKLVTLHLKQDKLQEAIESSKKYKQSHLARVVSAGLYEFQHDLQSGTADVV
                     GHDPIEAAERAIEREALMTTADMRKGLSGLATIGTTSPFIGLFGTVIGIINAFRGMAM
                     TGSGGIAAVSTGIAEALITTALGLFVAIPAVWLFNIFTNKIERFQVEMSNSASELIDY
                     FIKRRGAGVNLSARAS"
     gene            complement(4762..5499)
                     /locus_tag="VF958_07380"
     CDS             complement(4762..5499)
                     /locus_tag="VF958_07380"
                     /inference="COORDINATES: protein motif:HMM:NF015506.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="HEX9148967.1"
                     /translation="MFEDVLIESAKQKGRGRKALSLPVSLALHLVVIGLAVGASIWFV
                     EDVPEPPIPVTFYAAAPPPPPPPPAAAPKPAASKPEAPKQLPVKPSEMTAPTVIPEKS
                     PQPLAAPEPEAPASEGVEGGVEGGVPGGVMGGVLGGVVGGTGPGLEGEPLRVGGDVKA
                     PQLVNRVEPSYPEAARKARMEGVVILEAIITASGNVEDVKVLKSVNPLLDAAATRAVS
                     QWRYRPATLNGRAVRVYLTVTVTFNLH"
     gene            complement(5532..6338)
                     /locus_tag="VF958_07385"
     CDS             complement(5532..6338)
                     /locus_tag="VF958_07385"
                     /inference="COORDINATES: protein motif:HMM:NF025910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX9148968.1"
                     /translation="MIAFAMLATGCNKLKARTEIKKGNEFFKAGQYQTALAAYQDALR
                     LDPNEPKLHKNIGLAYMGMYQPGSKHPKDLEYAAKAIEHLKTYTETVPTDRKAREFLV
                     SMYLATERYDDAIAFYQGLVKENPSDVKAMQSLAQMYFKKGDVEHALEWLRKRLAAET
                     SPAAKAEVYYFIGVQAWDRSYNFPNIPLPERARIVDEGIDALNKALQIRPEYFEALSY
                     LNLLYREKAKIDPDPAKKQEFTDTANRYLAQALELRKKTLAATPTPEPAR"
     gene            6651..>7409
                     /locus_tag="VF958_07390"
     CDS             6651..>7409
                     /locus_tag="VF958_07390"
                     /inference="COORDINATES: protein motif:HMM:TIGR03436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HEX9148969.1"
                     /translation="MKKAALLPALAATVLFAQAAPPATTQEKRPPAVSSQKPPAEPAK
                     PAPASPSDSSHLPPISERVEVSVTNVDVIVTDSKGNRVPGLTREDFEVIQDGVPQAIT
                     NFYAVAGGKLLLEDGKTVPLDSPAAEAEVPRELKTRYIVYIDNLNIQPQNRNRMFKRL
                     KEFISANVGPHAEAMVVTYTRSLKVKRRFTSEKGDVLNAIEQTEMETGGGTTLAGERR
                     DAISQINDAQSEDQAIRIARSHSQSLRNDLEFTVD"
BASE COUNT         1248 a         2415 c         2449 g         1297 t
ORIGIN      
        1 cggggtcgga gctcttactc tccgcggacc tgattctcgg tctgccgggg cagacgtcga
       61 cgagcttccg gcgcagcgtg gaggcggtcg gcgcggccgg tttcggccat ctctcgatct
      121 acctgctcga gaccgacaag tcacggtctc tggatgagga caggcgcgcg catcccgaac
      181 ggtatctttc cgacgacgaa caggcggatg cctggatcga agcgggcgag gctctcgcgg
      241 ctcggggtct ccgtcactac gagatctcga actgggcgcg gccgggggac gaagctcgac
      301 acaacgtcaa gtactggacg cggacgccga cgctcggtct cggcatctcc gcccacgagc
      361 tctgggccga tcgccgtcgc gccaacgtct ccgcgatccc gcagtacgtc tcggcgctgt
      421 cggagggtcg ccggccgctc gcgctcgatc agccgatccc ggaggcggaa gcggcgaagg
      481 accgactcgt cctcggtctc cggctcgccc ggggagtcga cgcgggagaa atcgaagggt
      541 ggatcgcggt cgagggcgac ccgctgctgc ccgaagacta ccgggcgtgg ttcgaggccg
      601 gactgctcga aagggagaac ggtcgcgtcc gtttcagcga acgcggcttt ctcctgtcga
      661 acgaggtcct atgtcggttt gtgtgaccgt cgcgctcgct cgcatcggga tctgctcacg
      721 caccggcacg cggctcgggg acggccctcg accgcatgcc tgatccctct ctcgctcgcg
      781 ctcccgcggc gcgaggccga ttcctctgac ggccggcctc gtccttagat aaacagggga
      841 gcgagcacga gcgtgatcgt tgcgagcagc ttgatcagga cgtgcagaga cggtcccgcg
      901 gtgtccttga acggatcgcc gacggtgtcc ccgacgacgg ccgccttgtg ggtcggcgag
      961 ccctttccgc cgagcgcgcc gctctcgatg aacttcttgg cgttgtccca ggcgccgccg
     1021 gagttgttca ggaaggtcgc gagcacgacg ccggcgatcg tgccgaccat gagaagcgcg
     1081 gcgaccgcct cggcgccgac gtggaagagc cggaagaaca gccccacggc gatgggcatg
     1141 ccgacggcca cgaggcccgg cgcaatcatg gcccggagcg cgccgcgcgt gacgatgtcg
     1201 acggtccggc cgtagtcggg cttctccgtt ccctgcatga ttccggggtg cgccttgaac
     1261 tggtcgcgga cgtcctgaat gacgacccct gccgccttac cgacggcccg gatcgcgaga
     1321 cccgagaaga agaagacgag catcgccccg agcagtccgc cgatgaagac ttcgggcttc
     1381 gcgatgttca ccgccgcgag cggcgacccg tagttcttga gctcgtccag gtacgccgag
     1441 aacaggagaa aggcggcgag cgccgccgag ccgatcgcgt accccttcgt cagggccttc
     1501 gtcgtgttgc cgaccgcgtc gagacggtcc gtcttgcgac ggatctcctc cggttggcgg
     1561 gacatctcga cgatcccgcc ggcgttgtcg gtgatcgggc cgaaggtgtc catggcgagg
     1621 atgtaggccg ccgttccgag catccccatc gtcgcgacag ccgtcccgaa gaggccgccg
     1681 cccggaagcg cccacgctcc gatcttgaac gacgtgagga tcgcgagcga gatcgccaac
     1741 accggaagca acgtgcactc gaagccgacc gcgactccgg cgatcacgtt cgtcgccggg
     1801 cccgtctggg aagcattcgc gatctcgcgg accgggcggt acttgtactc cgtgtagtac
     1861 tgcgtgatgt agacgaaggc gacggaggtg aggacgccga tgattccaca gaggaagaac
     1921 ttccaccacg cctcgggggc cgccgggttg tagaggagcc accgcgtcgc gaggaagaag
     1981 ccgccgatcg cgagaagagc agtgaggtag tacccgcggt tcaacgcctg catgggatcg
     2041 cggtcttcgc gcgtatgcac gaagaagatt cccacgatcg acgcgagaag cccgaaggcg
     2101 cgtgcgacga gcgggaagag catgactccg acgattccgg cggcgaatcc catccccgcg
     2161 cgttccgacg cgccggcgag cgtcgcgccg agaatcatcg ctccgatgtt ctcggcggcg
     2221 gtcgattcga agaggtcggc accgcgcccc gcgcagtcgc cgacgttgtc cccgacgagg
     2281 tccgcgatca cggcggggtt ccgcggatcg tcctcgggaa tgccggcctc gaccttgccc
     2341 acgaggtcgg ccccgacgtc ggcggccttc gtgtagatgc cgccccccag ctgggcgaag
     2401 agggcgacga acgacgcgcc gaatccatac ccgacgatca ggagcgggat ctggggcggg
     2461 ggaacgtcgg tcaggtggct cacgagccag tagagcccgc cgacgccgaa gagggacatc
     2521 gccacgacga acagaccgga caccgcgccg ccccgaagag cgatgcgcag cgcgtcgttc
     2581 agcgaggtcc gggcggcgga cgcggtgcgg atgttcgagc ggatcgagat ccacatcccg
     2641 atgtacccgg cgatgaccga gcacgtcgca ccgagagcga aggagatcgt cgtccagagc
     2701 gcgagtttga gcgccgtcgg gacgggatcg aagtcgtgat gcgcgcgaac gaatccatac
     2761 agcaggaaga tgacggcggc gaggacggcg gcgaggagga caatcgtcag gttctggcgt
     2821 cggaggaagg cttcggcgcc ttccttgatc gcattggaaa tctcccgcat ggccggcgtg
     2881 ccggtatcgc gcttcaaaac ccaggagatc aggtaaccgg cgacgaagag cgagccgatc
     2941 gagaagacca tgatgagggg gagagcgagg tcgtatgaca tgccaaactc ctcttttgcg
     3001 acttcgggat cggccgtctc gaacgagcgg gtctggtccc cgaaggggtc agatcccgat
     3061 catatttact gcgcgggagc gggcgccggc ggcgcggcgg cgaggccttc gaccggcacg
     3121 gtctcgagga cgatcccgag gttcttcgcg ccagcggtgc gcacgatgtc catgacccgc
     3181 gcgacctcgc cgtaagtcac tttatcgtcg gccgagaaga acaccgtctt gtcgcggcgg
     3241 ttcttcagga tctccgtgag ccgaagcgac agcgactgcg cgtcgagctg ctccttgttc
     3301 agatagatgc gcccttgagg ggtcagggag acgatcagtt ggtccagcga cggcggcgga
     3361 ttctcgaccg tcgccttcgg cggcactttc acgtcgtagc ccatctggag cagcggcgtg
     3421 atgaccatga agatgatgag gaggacgagc acgatgtcga cgaggggagt gatgttgatg
     3481 tcactcttgt agccccccgc cgccgggccc ccgaccgctg ccgccattgg tagtgctcct
     3541 ccgcgctccc ggccgctcta gccggccgtg cccttctgct tcttgtcgac gatgagcccg
     3601 acgtcctgga agcccgcttc tttcgtgatg cgcatgacct ctttgacctg gccgtatttc
     3661 agccgctggt ccgccttgat gacgatctcc gccgccggat cccgctggta catctcgtcg
     3721 agctccttct ggaattcttc cttcggaagc cacttggtgt cgatgtagta cttcggcggg
     3781 tcgccccacg ccacggtgat gagtttctgc ttcgcgttct ccggcttctt gtccgggttc
     3841 tcggtgaccg ggagctggat cggaattccc ttctggagca tgggagtgac gaccatgaaa
     3901 atgatcagca gaacgagaca cacgtcgacg aggggagtga cgttgatgtc gctctgcaga
     3961 ttgtgacgcc cgcctccgcc ccccattccc atggccggac ctcctgacta ggacgcgcgc
     4021 gcggagaggt tgacgccggc tccgcgccgc ttgatgaagt agtcgatgag ctccgaggcc
     4081 gagttggaca tctcgacctg gaagcgctcg atcttgttcg tgaatatgtt gaagagccag
     4141 accgccggaa tcgcgacgaa gagaccgagc gccgtcgtga tgagcgcctc cgcgatgccg
     4201 gtcgacaccg ccgcgatacc gcccgagccc gtcatcgcca tcccgcggaa agcgttgatg
     4261 atcccgatga ccgttccgaa gaggccgatg aacggcgagg tcgtgccgat cgtcgccagg
     4321 cccgagagac ccttgcgcat gtccgccgtc gtcatcagag cttcccgctc gatcgcccgc
     4381 tccgcggctt cgatcgggtc gtggcccacg acgtcggcgg tcccgctctg gaggtcgtgc
     4441 tggaactcgt agagcccggc cgagacgacc cgggccaggt ggctctgctt gtacttcttc
     4501 gacgactcga tcgcctcctg aagcttgtcc tgcttcaagt gcagggtcac gagcttcgcg
     4561 aaggcgagcg actgtttctt cgccttggca aagtagatcc accgttcgat cgctacggtg
     4621 agggagtaga tcgacatcag gatgaggagc acggccacgc cttttgcgag cggcgtcatc
     4681 gtgttccaga gatcgacggg attgaaactc atgaaatgct ccttctcaag ttcgttctcc
     4741 ggtcaggtcc cgcgtccaat cctagtgcag gttgaaagtg acggtgacgg tcaggtacac
     4801 gcgtaccgcg cgcccgttca gcgtcgcggg ccgatacctc cactgtgaga ccgcccgagt
     4861 ggccgcggcg tcgaggaggg gattgacgga cttaaggacc ttgacgtcct cgacgttgcc
     4921 gctcgccgtg atgatcgcct cgaggatgac gaccccttcc atcctcgcct ttcgcgccgc
     4981 ctccggatag ctcggctcga cgcggttgac gagctgcggc gccttgacgt cgccgccgac
     5041 ccgcagcggc tcgccctcga gccccggtcc ggtgccgccc acgacgccgc cgagaacgcc
     5101 tcccatcact ccacccggaa cgccgccctc gacgccgcct tcgaccccct cgctcgccgg
     5161 cgcttccggc tccggggccg cgagcggctg gggactcttc tcggggatga ccgtcggagc
     5221 cgtcatctca gaaggcttga ccgggagctg cttgggtgcc tccggcttgg aagccgccgg
     5281 cttgggggcg gcggcgggcg gaggcggtgg cggcggcggc gccgccgcgt agaaggtcac
     5341 cggaatcggc ggctccggca cgtcttcgac gaaccagatc gaggccccga ccgcgaggcc
     5401 gatcacgacg aggtggagcg cgagcgacac cggaagcgac agggccttcc gacctcgccc
     5461 cttctgtttg gccgactcga tcaggacgtc ttcaaacatg cagtatctcc ccgcccgaaa
     5521 agaccccgcg cctagcgggc cggttccggc gtgggcgtcg ccgccagggt cttctttcgc
     5581 aattccaatg cctgcgccag gtagcggttc gccgtgtcgg tgaactcctg cttcttggcc
     5641 ggatcggggt cgatcttggc cttctcccgg tagagcaggt tcagatacga gagagcctcg
     5701 aaatactcgg gtcggatctg gagcgccttg ttcagagcgt cgatgccctc gtcgacgatg
     5761 cgcgcccgct ccgggagcgg aatgttgggg aagttgtacg accggtccca ggcctgcacg
     5821 ccgatgaagt aatagacctc cgccttcgcc gccggtgacg tctccgcagc gagacgcttg
     5881 cgcagccact ccagggcgtg ctcgacgtcc cccttcttga agtacatctg ggcgagcgac
     5941 tgcatcgcct tcacgtcgct cgggttctcc ttgacgagcc cctggtagaa ggcgatcgcg
     6001 tcgtcgtatc gctccgtcgc gaggtacatc gacacgagga actcgcgggc cttgcggtcg
     6061 gtcgggaccg tttcggtgta ggtcttcaga tgttcgatcg ctttggccgc gtactcgagg
     6121 tccttgggat gcttcgagcc cggctggtac atccccatgt acgcgaggcc gatgttcttg
     6181 tggagcttcg gctcattcgg gtcgagccga agtgcgtcct ggtaggccgc gagtgccgtc
     6241 tggtactgcc cggctttgaa gaactcgttg cccttcttga tctccgtgcg ggccttgagc
     6301 ttgttgcagc cggtggcgag catcgcgaag gcgatcacca gtccggcgac ggtgcgcctt
     6361 cgcgaaaatg aacgttcccg gtgcacgaac atgctggagc cgctcctctg gtcttttctt
     6421 tcgctcgccg gacggggttg cccggcccgg aggcggaatt ctgttgaatt cggggcggca
     6481 aagcaacacc cgattttcgc gcgggcgctc ccgcaaaacc cggcgcaccc ggggtttccg
     6541 gttttccgcc tcgttcccgc cggtctggcg tcggctggct ctcgcgcagc gcggccgggc
     6601 gcggggaata atcgcgcgtt ggacaaactt gccgttcgag gaggatcctt ttgaaaaaag
     6661 ctgctctcct gcccgccctg gcggcgaccg tgctgtttgc gcaggccgcc ccgcctgcaa
     6721 cgacgcaaga gaagcgtccc ccggccgtct cgtctcagaa gccgcccgcc gagccggcga
     6781 aaccggcccc ggcgtccccg tccgattctt cgcacctgcc gccgatctcc gaacgggttg
     6841 aggtctccgt cacgaacgtc gacgtcatcg tcaccgactc gaagggaaac cgcgtcccgg
     6901 gtctgacccg cgaggatttc gaggtcatcc aggacggcgt cccgcaggcg atcacgaatt
     6961 tctatgcagt cgccggggga aagctgctcc tcgaggacgg aaagacggtg ccgctcgact
     7021 ctcccgccgc ggaggcagag gtcccgcgcg agctcaagac acgctacatc gtttacatcg
     7081 acaacctgaa catccagccg cagaaccgca accggatgtt caagcgcttg aaggaattca
     7141 tctccgccaa cgtcggacct cacgcggaag cgatggtcgt gacgtacacc cgctccctga
     7201 aagtgaagcg gcgctttacc tccgaaaaag gggatgtgtt gaacgcgatc gagcagacgg
     7261 agatggagac cgggggaggg acgaccctcg ccggcgagcg tcgcgacgcg atctcgcaga
     7321 tcaacgacgc gcagtccgag gaccaggcga tccgcatcgc gcgctcccac tcccagtcgc
     7381 tgcgcaacga tctcgagttc accgtcgac
//
LOCUS       DASWAV010000355         6720 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U2665
            k141_619545, whole genome shotgun sequence.
ACCESSION   DASWAV010000355 DASWAV010000000
VERSION     DASWAV010000355.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960316
            Sequence Read Archive: SRR10260007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 6720)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6720)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:47:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,929
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6720
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_619545"
                     /isolate="SMAG_U2665"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.36444 N 132.31889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            79..1077
                     /locus_tag="VF958_07395"
     CDS             79..1077
                     /locus_tag="VF958_07395"
                     /inference="COORDINATES: protein motif:HMM:NF019911.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEGA domain-containing protein"
                     /protein_id="HEX9148970.1"
                     /translation="MKRLTFALAFVAFALLAAPVTAAPRGGHGGSGRHVGGHGGSGRH
                     VIVVRPGFWGWGFGSWYDPYWYGYGYGYGPYYRGYPGRYGYGYGTPGNWAVVDTDVSP
                     ESARVYLDGQYIGTADDFDGYPDYLYLRRGRYRLEFRLEGYQPRTVEIDARPGVKIDV
                     DDKLARVPGAPKYGSYDTPEPQGGVRRFWGKRNNVSVEVTDEYDISGDGRHYGERRER
                     YQADDEPTMDRDRDRRDSEVDRDREDSRDEWRADRNAAETGDTRLVLDIHPSDASIYL
                     DDRFIGSASELGSDSGVAVAPGRHTLVVSRPGYRERRVEVNVSRGEAQNIEITMER"
     gene            complement(1330..2262)
                     /locus_tag="VF958_07400"
     CDS             complement(1330..2262)
                     /locus_tag="VF958_07400"
                     /inference="COORDINATES: protein motif:HMM:NF024575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9148971.1"
                     /translation="MSSVSPVLERLTARLEQFPGALLLHGASAKALEATSLSLAARLL
                     CAGDDRERRCESCRRVLHGFHPDLLSIEPAGVQIRIDRIREAIAFGAGRPYESTRRVV
                     RVSRAELLGPEAANALLKTLEEPGARLHWILTTRRPESLLPTVRSRCVSLSVASPSVG
                     ERVRAWREAGISEDDAADLAAFAPDADSADIEGLEEARRFRSDAVDALRAGIVDGKLS
                     PLVLLAETLARAEARQLTVVVELLADAALLAAGVSPDLVRHRAVTGALSAIARSAGSL
                     ALEKAATTAADYPPDNRRGNRRLHMEKVLLSLAR"
     gene            complement(2259..2972)
                     /gene="tmk"
                     /locus_tag="VF958_07405"
     CDS             complement(2259..2972)
                     /gene="tmk"
                     /locus_tag="VF958_07405"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012611193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HEX9148972.1"
                     /translation="MLAEGEDPAVERSLVGSTNRRQRIADTFFSVPFITFEGVEGSGK
                     STQLTRAARRLEARGYASLATREPGGTRIGRILRGVVLGKANRELDPIAEWLLFEADR
                     CQHVREVLRPALDRGLFVLCDRYSDATEAYQQVGRRLDGRLVARVDEVARDGLVPDLT
                     LLFDLDPRAGLDRKNRRGGRGVGRFEETELAFHRKVRRAYLAIAKREPKRVRVLDARR
                     PVGELARETWKALAERFDL"
     gene            2980..5550
                     /locus_tag="VF958_07410"
     CDS             2980..5550
                     /locus_tag="VF958_07410"
                     /inference="COORDINATES: protein motif:HMM:NF018884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HEX9148973.1"
                     /translation="MNAYPLKMAESSRGRVLYLAIGFAGLALAVASIADMFAPRPYDG
                     IIPVPYSRGGIEVRAVVPGSPAAKAGIRAGDCVLGIGKRIVGSTSDASAELRKHAIGE
                     LVPYIYQRGRCGAETRGELPTVRLHLSSERLGGTTYVYSAGLGFLFFFIGFFVFKRRP
                     EDRAARIFFLLCVLFLLFFVCRLRPASYWWIDLFVQNTGTVSLFLLPAVFLHFFLIFP
                     RPKQLTFARADEWTGEPPPRWKEKLQDFLSASPSLFYFLYSIPPVVFLYDVMRQLRGH
                     RVAILSGAPLSSWVLLGDYLVLGLIALAHSAFTLPDARERRQAFHVFIGTIVGIAPFV
                     LFGIVLPSAFNIEDYVFYGIVPMIFIPLTFAYAIVRFQMLNVRVIVRRTFLYAATTAI
                     LLGLYALAVAMANVIFSESRLSSSPLFNFGFFLVGISLFEVLRRRLQTPLDKLFFREK
                     FDYQKALLEMSEAITGELDLGRIADFLTGRVAATMRLDRVSIWLRDDQGWLERRGRRD
                     DRLSPTAEIRRVLRETGNPAPPDELLERVSDEESRRFCERVSAEGFHLLVPLVYRDRL
                     MGLLALKEKLSGERFDRDDQTLLSTLANQSALAIETATLHDEMTRQAELRRDLEIARD
                     IQMSLLPRSLPIVAGFSFLGGSLPAKVVGGDFYDFISFDDHRLGVVIGDVSGKSVPAS
                     LLMVASKEIVYSRALTTRDPGTLFQESNRRIYAIKRRMFVSLGYFLLDPDSMSLRYSI
                     GGQPLPMILRSGDGGPRFLEPPNHRLPLGALKEVPYDTREMFLKRGDLLFFYTDGFTE
                     AMDNEMNPYGEERLMESLSSRRSAPLDEIARGLLSDIRTYVAGAEQYDDMTFLFMRVE
                     "
     gene            5716..6513
                     /locus_tag="VF958_07415"
     CDS             5716..6513
                     /locus_tag="VF958_07415"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX9148974.1"
                     /translation="MTKRRAISLFGILLLVAAAGVAAALLLTNRREATTSSDAAYRLW
                     RDAIENERRFYMKEARAGFAKALELDPDFVAAMLGLARQSTRDQALSLVHRAERLKSR
                     LPERERLHVDLAVANLEKGPDERLKIARQILRAYPDDFRATTTVAMDYARKGQMEEAV
                     RVFTQLLEMDPNSADAYNMIGYYYGYRGEYEKAIENLKKYQFMAPDQANPHDSLGEIQ
                     AYSGHYDEAIQNLNRAIAIKPDFYAPMTISASPTRGKATSLGLSRTT"
     gene            6510..>6720
                     /locus_tag="VF958_07420"
     CDS             6510..>6720
                     /locus_tag="VF958_07420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9148975.1"
                     /translation="MKAADAMPLESERPYDLYRALRVAIISKDVAAGRTLLPRFEKIP
                     RDEHWELVKPGVLAAYDLLEGRPQEA"
BASE COUNT         1080 a         2290 c         2172 g         1178 t
ORIGIN      
        1 aacgggagac ttggcgcaag cgccgcgcct tgtgaccatg gccccgggtt tgctcttcga
       61 atctgcggag gcatttccat gaagaggctc acattcgccc tcgcgttcgt cgccttcgct
      121 cttctcgccg cgccggtgac ggccgcgccg cgcgggggac acggcggatc cgggaggcac
      181 gtcgggggac acggcggatc cggacggcac gtgatcgtcg tgcgacccgg tttctggggt
      241 tggggattcg gttcctggta cgacccatac tggtatggct acggctacgg gtacggccct
      301 tactaccggg gctatcccgg gagatacgga tacgggtacg ggacgccggg taactgggcg
      361 gtcgtcgata ccgacgtctc acccgagagc gcccgcgtct acctggacgg gcagtacatc
      421 gggacggcgg acgacttcga cggctatccc gactatctgt atttgaggcg gggccgctat
      481 cggctcgagt tccgtctcga gggatatcag ccgcgcacgg tcgagatcga cgcgcgcccg
      541 ggcgtcaaga tcgacgtcga tgacaagctc gcccgggttc cgggagcgcc gaagtacggc
      601 agctatgaca cgcccgagcc acagggtggt gtgcggagat tctggggtaa gcgcaacaac
      661 gtttcggtcg aagtgaccga cgagtacgac atctccggcg acggccgtca ctacggtgag
      721 cggcgggagc ggtaccaggc ggacgacgag cccacgatgg atcgggatcg cgatcgccgt
      781 gacagcgaag tcgaccgcga ccgcgaagac tcacgagacg agtggcgggc cgatcggaac
      841 gccgccgaaa cgggcgacac gcgtctcgta ctcgacatcc acccttcgga cgcgtcgatc
      901 tatctcgacg atcgcttcat cggctccgca tcggagctcg gctccgactc cggggtcgcg
      961 gtcgcacccg ggcgccacac gctcgtcgtc tcccgtcccg gatatcggga gcgccgggtc
     1021 gaagtgaacg tatcgcgggg cgaggcgcaa aacatcgaga tcacgatgga gcgctgaatc
     1081 tccgactttc gtcgcccccg accttcgcat ctcctacgat tcctcgtttt cggtctatac
     1141 tgccgaattg aaggaattag ggggaggcgc gaaggtccgg cggcgtcagt cacggtggtt
     1201 gaccccacct tctgccagtt tcggtagctc ccaggtccgc actcttcccg aaatccttcc
     1261 gatcccgccg gatcggaggg cctccccctt agttccttcg atctcgcgag cgggggaccg
     1321 gggcgcggcc tatcgcgcga ggctgagcag aaccttctcc atgtgaagtc gccggttgcc
     1381 gcgccggttg tcgggcgggt aatcggctgc cgtcgtcgcg gccttctcga gcgcgaggga
     1441 gccggcggaa cgcgcgatcg cggagagagc gcccgtcacc gcgcggtggc gcacgaggtc
     1501 gggggagaca ccggcggcga gaagcgcggc gtcggcgaga agctcgacga cgaccgtgag
     1561 ctgtctcgcc tccgcccgag cgagcgtctc cgcgagaaga accagaggcg agagctttcc
     1621 gtcgacgatc cccgcgcgaa gagcgtccac cgcatccgat cggaaacggc gggcctcttc
     1681 gagtccctcg atgtcggcac tgtccgcatc cggcgcgaac gccgcgagat cggcggcgtc
     1741 gtcctccgat atgcctgcct cacgccacgc gcggacgcgc tcgccgaccg agggcgaggc
     1801 taccgagagg gaaacgcacc gggaccggac cgtcggaaga agactttcgg gccgtcgcgt
     1861 ggtcaggatc caatgcagcc tggcgccggg ctcttcgagc gtcttcagga gcgcgttcgc
     1921 agcctccggc ccgagcagct ccgcgcgcga gacccgcacg acgcgccgcg tgctttcgta
     1981 gggcctgccc gctccgaatg cgatcgcttc gcggatgcga tcgatccgga tctggacacc
     2041 cgcgggttcg attgacaaca ggtcggggtg gaaaccgtga agcacacggc ggcaggactc
     2101 gcaccggcgc tcgcggtcgt cccccgcaca gagaagtcgc gccgcaaggg aaagcgacgt
     2161 cgcctcgagc gctttcgccg aagcgccgtg gagcagcaac gcgcccggaa actgctccag
     2221 acgcgcagtc aaacgctcga gaaccggcga gacggagctc acaggtcgaa gcgctcggcg
     2281 agcgccttcc acgtctcgcg agcgagctcg ccgaccggcc ggcgcgcgtc gaggacgcgc
     2341 acgcgcttcg gctcccgctt cgcgatcgcg agataggctc gccgcacctt ccggtggaac
     2401 gcgagctcgg tctcttcgaa ccgcccgacg ccgcgtcctc cacggcggtt cttgcggtcg
     2461 agtcctgcgc gcgggtcgag gtcgaagagg agcgtcaggt ccgggaccag cccgtcgcgc
     2521 gcgacctcgt ccacccgcgc gacgagccgg ccgtccaggc gtcggccgac ctgctgatag
     2581 gcctcggtgg cgtcggagta cctgtcgcag aggacgaaga gcccgcggtc gagcgccggg
     2641 cgcagaacct cccggacgtg ctggcagcgg tccgcctcga agagaagcca ctccgcaatc
     2701 ggatcgagct cgcggttcgc cttgccgagg acgacgccgc ggaggatccg gccgatcctc
     2761 gtgccgcccg gctcgcgcgt cgcgagcgat gcgtagcccc tcgcctcgag gcggcgtgcc
     2821 gccctcgtca gctgcgtcga tttgcccgag ccctcgacgc cttcgaacgt aatgaagggc
     2881 acggagaaga aggtatcagc tatccgctgc cggcgattcg tggaaccgac gagcgaccgt
     2941 tcgacggccg ggtcttcgcc ctcggctaac attgcccgat tgaacgcgta tcccttgaag
     3001 atggcggaat cctcgcgcgg ccgcgtcctc tacctcgcga tcggattcgc gggcctggct
     3061 ctcgccgtcg cgtcgatcgc ggacatgttc gcgccgcgcc cctacgacgg aatcattccg
     3121 gtcccgtaca gccgcggcgg aatcgaggtc cgcgcggtcg tccccggcag cccggccgcg
     3181 aaggccggaa ttcgcgccgg agactgcgtg ctcgggatcg gcaagaggat cgtcgggtcg
     3241 acgtcggacg cgtccgccga gctccgtaag cacgccatcg gggagctcgt tccctacatc
     3301 tatcagcggg gccgttgcgg cgcggagacc aggggagagc ttcccaccgt ccggttgcac
     3361 ctctcctcgg agcgtctcgg cgggacgacg tacgtctact cggcggggct cggtttcctg
     3421 ttcttcttca tcgggttctt cgtcttcaaa cggcggccgg aggatcgcgc ggctcggatc
     3481 tttttcctgc tctgcgtcct cttccttctc ttcttcgtgt gccggctgcg gcccgcttcc
     3541 tactggtgga tcgacctgtt cgtccagaac acgggcacgg tgagcctttt cctcctgccc
     3601 gccgtcttcc tgcacttctt tctgattttt cctcgcccga agcagctcac gttcgcgcgg
     3661 gcggacgaat ggacggggga gccgccgccc cgatggaagg agaagctgca ggacttcctg
     3721 tcggcgagcc cgagtctctt ctactttctc tattcgatcc cgccggtcgt tttcctctac
     3781 gacgtcatgc ggcagctccg cgggcaccgc gtcgccatcc tctcgggagc gccgctctcg
     3841 tcgtgggtgc tgctcgggga ttatctcgtc ctcgggctga tcgctctcgc gcactccgct
     3901 ttcacgctcc cggatgcgcg cgagcggaga caggcattcc acgtgttcat cggcacgatc
     3961 gtcgggatcg cgcccttcgt tcttttcggg atcgtgctcc cctcggcgtt caacatcgag
     4021 gactacgttt tctacggcat cgtcccgatg attttcattc cactgacgtt cgcctacgcg
     4081 atcgtccgct tccagatgtt gaacgtgcgc gtgatcgtgc gccgcacgtt cctgtacgcg
     4141 gcgacaaccg caatcctgct cggcctctac gcgctcgcgg tcgcgatggc gaacgtcatc
     4201 ttttccgagt cgcgcctgtc ctcctcgccg ctcttcaact tcggcttttt cctggtgggc
     4261 atctcgctct tcgaggtcct gcggcgccgc ctccagacgc cgctcgacaa gctcttcttc
     4321 cgcgagaagt tcgactacca gaaggccctg ctcgagatga gcgaggcgat cacgggagag
     4381 ctcgacctcg gtcgcatcgc ggattttctg accggccggg tcgcggcgac gatgcggctc
     4441 gaccgcgtct ccatctggct gcgcgacgac cagggctggc tggagcggcg gggacgccgc
     4501 gacgacaggc tctctccgac ggcggagatc cggcgcgtcc tccgcgagac ggggaaccct
     4561 gctccgcccg acgagctcct cgagcgtgtt tccgacgagg agagccggcg cttctgcgaa
     4621 cgtgtgtccg cagaagggtt tcaccttctc gtcccgctcg tctatcggga tcggctcatg
     4681 ggattgctcg ccctgaaaga gaagctctcg ggagaacggt tcgaccggga cgatcagacc
     4741 ctcctctcca ccctggcgaa ccagtcggcg ctcgcgatcg agacggcgac gctacacgac
     4801 gagatgaccc gtcaggcgga gctccgacgg gacctcgaga tcgcgcggga cattcagatg
     4861 agtcttctgc cgcggtcgct tccgatcgtc gcgggctttt ctttcctcgg cggcagcctg
     4921 ccggccaagg tcgtcggtgg agacttctac gacttcatct ccttcgacga ccaccggctc
     4981 ggcgtcgtca tcggagacgt ttcgggcaag tcggtgccgg cgtccctcct catggtcgcc
     5041 tcaaaggaga tcgtctactc gcgcgccctg acgacccgcg atcccggaac cctcttccag
     5101 gaatccaacc ggcgcattta cgcgatcaag cggaggatgt tcgtttccct cggatacttc
     5161 ctgctcgacc cggattcgat gagcctgcgg tactcgatcg gtggccaacc tctcccgatg
     5221 atcctgcgct cgggagacgg cggcccgcgc ttcctcgagc cgccgaatca ccggctgccg
     5281 ctcggggcgc tgaaagaggt gccttacgac acccgcgaga tgttcctgaa acggggggac
     5341 ctgctcttct tctacaccga cggtttcacc gaggcgatgg acaacgagat gaacccctac
     5401 ggggaggagc gcctcatgga gtcgctctct tcgcggcgct ccgcaccctt ggacgagatt
     5461 gcccgggggc ttctgtccga catccggacc tacgtggccg gagccgagca gtacgacgac
     5521 atgacgtttc ttttcatgag ggtggaatga cgcccgatcc ccgccccgtt tcgtccaccg
     5581 ggcggaggga gctgtcccgt tctccggccg ccggtccccg ggaggtaaaa ccatggctac
     5641 actgacgttg tcgaggccgg cgatcgagcc tggaggcgaa acctcggccc gtcccggccg
     5701 tatcatcggt gtgcgatgac gaagcgccgc gcaatctctc tgttcgggat tctccttctc
     5761 gtggccgcgg ccggggtcgc ggccgccctc cttctgacga accgccgcga ggccaccacc
     5821 tcctccgacg ccgcgtaccg gctctggcgc gacgcgatcg agaacgagcg gcgcttctac
     5881 atgaaggaag cccgggccgg cttcgcgaaa gcgctcgaac tcgaccccga cttcgtcgcg
     5941 gcgatgctcg gtctcgcccg ccagtccacc cgcgaccagg cgctctcgct cgttcaccgc
     6001 gccgaacggc tgaagagccg gctgcccgag cgcgaacggc tccacgtcga tctcgccgtg
     6061 gcgaacctcg agaaggggcc ggacgaacgt ctgaaaatcg cccgccagat cctccgcgcc
     6121 taccccgacg acttccgggc gacgaccacc gttgcgatgg attacgcccg gaagggacag
     6181 atggaggagg cggtccgcgt cttcacgcag ctcctcgaga tggaccccaa cagtgccgat
     6241 gcctacaaca tgatcgggta ctactacggt taccgtggcg agtacgagaa ggcgatcgag
     6301 aacctgaaga agtaccagtt catggcgccc gaccaggcga acccgcacga cagtctcggg
     6361 gagatccagg cgtactccgg gcactacgac gaagcgatcc agaacctgaa ccgggcgatc
     6421 gcgatcaagc cggacttcta cgcgcccatg accatctcgg cgtcgcctac gaggggaaag
     6481 gcgacttcgc tcgggctttc gagaactacg tgaaggccgc cgacgcgatg ccgctcgaat
     6541 ccgaacgtcc ctacgacctc taccgggcgc tccgcgtcgc gatcatctcg aaggacgtcg
     6601 cggcgggtcg aaccctgctt cccaggttcg agaagattcc aagagacgaa cactgggagc
     6661 tcgtgaagcc gggcgttctc gccgcctacg atctgctcga aggccggccg caggaagccg
//
LOCUS       DASWAV010000356         3754 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U2665
            k141_258273, whole genome shotgun sequence.
ACCESSION   DASWAV010000356 DASWAV010000000
VERSION     DASWAV010000356.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960316
            Sequence Read Archive: SRR10260007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 3754)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3754)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:47:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,929
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3754
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_258273"
                     /isolate="SMAG_U2665"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.36444 N 132.31889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1254
                     /locus_tag="VF958_07425"
     CDS             <1..1254
                     /locus_tag="VF958_07425"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013375493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HEX9148976.1"
                     /translation="ITKHEGQADNTPQEFDPKTGALYYLSNEDSDFARVRRYDAATGK
                     SEDVEKPAWDPLFVRFSKNGRYRATGINDDGRTVVHVLDTQTGREVALPKLPAGDITT
                     VRFADSEKRMAFYVSGDRSPANLYVYEFGAKVPVRLTDSQNKEIDPADLVESQVVRFK
                     SFDGTVIPSIYYKPLQATAKNRVPALVWVHGGPGGQTRKGWSATIQYLVNHGYAVLGI
                     NNRGSSGYGKSFYVADDQKHGKEPLTDCVEGKKYLASLPYVDADRIGILGGSYGGYMV
                     LAALAFRPEEFAVGVDIFGVSNWLRTLQGIPKWWEAQRLALYKEIGDPEKDEAMLREI
                     SPVFHADRIRRPLLVLQGANDPRVIKPESDDIVAAVKKNGVPVEYIVFPDEGHGFTKK
                     KNQIEGYGAILTFLDKYLKGAAAAR"
     gene            1251..2501
                     /locus_tag="VF958_07430"
     CDS             1251..2501
                     /locus_tag="VF958_07430"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEX9148977.1"
                     /translation="MKTTRRQFVKTTGAGTVAATLIPSLAGARAPAVSGSKPYDVAVV
                     GAGVFGAWIAYRLRRAGRRVALVDAYGPGNARASSGGQTRVIRMGYGDQEIYTRWSMR
                     SLDLWKSLFGKAGRAFFQPSGVLWMARGEDPLTTKTLATLKRLGVAHERLERSELERR
                     WPQIDFGPITWAIHEPRSGFLMAFHSVQAVVQFAMADGVEYLQEAVMPPTGKGRLDSV
                     ATQSGKTVAAGTFVFACGPWLPKLFPDLLGERIFPTRQEVFYFGPPRGDARFRSSDMP
                     VWVDFAEEIYGIPDFKGRGFKVAPDRHGPDFDPDTGARVITPETLATVREFVARRFPG
                     LKDAPLVGSEVCQYENTSNGDFLIDRHPERDNVWLVGGGSGHGFKHGPAVGEYVAARI
                     VEGGPVEPRFSLATKEKVQKRAVY"
     gene            2581..>3754
                     /locus_tag="VF958_07435"
     CDS             2581..>3754
                     /locus_tag="VF958_07435"
                     /inference="COORDINATES: protein motif:HMM:NF014758.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein"
                     /protein_id="HEX9148978.1"
                     /translation="MGASRRSGAVGRNVPRKEGLDKVAGRAKYVDDLSFPGMLHGRTI
                     RSTVPRGEITGIRLDFDRAGFTIADFQDIPGRNTIALIELDQPCLAEREIRHFAEPIL
                     LLAHDNRDALLDAERSITIDYRTAVPVYDPTRSPQCFKEIVIEKGELARGFAAADLVV
                     EGEYRAGHQEQLYIEPNGVIAVPEDGGVTVYGSLQCPYYVHRALKVLLGLPEDRVRVV
                     QAETGGGFGGKEEYPSMIAGHAAILALKAGRPVKLVYDRVEDMVATTKRHPAAIRHRT
                     GVTRDGRLTAIEIDVLMDGGAYMTLSPVVLSRGVIHATGPYRCDNVLVHGRVVMTNTP
                     PNGAFRGFGAPQTQFAAEVHMERIAEALGMDPVRLREVNAYRPGDTTATGQRLGRDC"
BASE COUNT          653 a         1266 c         1268 g          567 t
ORIGIN      
        1 atcacgaagc acgagggcca ggccgacaac acaccccagg agttcgaccc gaagacaggc
       61 gccctctact acctgtcgaa cgaggactcg gacttcgcgc gggtgcggcg gtacgacgcg
      121 gcgaccggga agtcggagga cgtcgagaag cccgcatggg atccgctttt cgtccgcttc
      181 tcgaagaacg gccgttaccg cgccacgggg atcaacgacg acggaaggac ggtcgtccac
      241 gtcctcgaca cgcaaacggg cagggaagtc gcccttccga agctgcccgc cggggacatc
      301 acgacggtgc gtttcgccga cagcgagaag cggatggcgt tctacgtcag cggcgaccgg
      361 tcgcccgcca acctctacgt ttacgagttc ggagcgaaag tgccggttcg actgaccgac
      421 tcacagaaca aggagatcga tcccgcggac ctcgtcgaat cgcaggtcgt ccggttcaag
      481 tcgttcgacg gcacggtcat cccctcgatc tactacaagc ccctccaggc gaccgcgaaa
      541 aacagggtcc ccgcgctcgt gtgggtccac ggcggtccgg gtggccagac gcgcaagggc
      601 tggagcgcga cgattcagta cctggtcaac cacggctacg cggtgctcgg gatcaacaac
      661 cgcggcagct ccgggtacgg caagagtttc tacgtcgccg acgaccagaa gcacggaaag
      721 gaaccgctca ccgattgcgt cgaggggaag aagtacctcg cgagcctgcc gtacgtcgac
      781 gcggaccgca tcgggatcct cggcggcagc tacggcggct acatggtcct cgcggctctc
      841 gcgttccggc ccgaggagtt cgcggtcggc gtggacatct tcggagtgtc caactggctc
      901 cgcacgcttc aggggatacc gaagtggtgg gaggcgcagc ggctggccct ctacaaggag
      961 atcggagacc cggaaaagga cgaagcgatg ctccgcgaga tctcgccggt ctttcacgcg
     1021 gaccggatcc gccggccgct cctcgtcctg cagggcgcga acgacccgcg cgtcatcaag
     1081 cccgagtccg acgacatcgt cgcggcggtc aagaagaacg gcgtccccgt cgagtacatc
     1141 gtgttccccg acgaggggca cggcttcacg aagaagaaaa accagattga aggctacggc
     1201 gcgatcctga cgttcctcga caaatacctg aagggggcgg ccgccgctcg atgaagacga
     1261 cgcgtcgcca gttcgtcaag accaccgggg cggggaccgt cgccgccacg ctcatcccgt
     1321 cccttgcggg tgcccgggcg cccgccgtgt cggggagcaa gccctacgac gtcgccgtcg
     1381 tcggagccgg tgttttcggc gcatggatcg cctaccgtct ccggagggcc ggacgccgcg
     1441 tcgcgctcgt ggacgcctac ggtccgggca atgcgcgcgc gagctccggc gggcagaccc
     1501 gcgtcatccg catggggtat ggcgaccagg agatctacac gcgctggtcg atgcgctccc
     1561 tggatctttg gaagtcgctt ttcggcaaag ccggccgggc gttctttcaa ccctccggcg
     1621 tgctctggat ggcgcgcggc gaggacccgc tcacgacgaa gacgctcgct accttgaagc
     1681 ggctcggcgt cgcccacgag aggctcgagc gctcggagct cgagcgccgg tggccgcaga
     1741 tcgacttcgg cccaatcacc tgggcgatcc acgaaccccg aagcgggttc ctgatggcct
     1801 tccattcggt ccaggcggtc gtccagttcg cgatggccga cggagtcgag tacctccagg
     1861 aagccgtgat gccgccgacc ggcaaagggc ggctcgactc ggtcgcgacg cagtccggaa
     1921 agaccgtcgc ggcgggcacc ttcgtcttcg cgtgtggtcc ctggctcccg aagctgtttc
     1981 ccgatctcct cggcgagcgc atcttcccca cgcggcagga ggttttctac ttcggtccgc
     2041 ctcggggcga cgcgcgcttt cgctcctcgg atatgccggt ctgggtggat ttcgccgagg
     2101 agatctacgg cataccggat ttcaagggac gcggcttcaa ggtggccccg gatcgccatg
     2161 ggcccgattt cgacccggac acgggcgcgc gcgtgattac gcccgagacc ctggccaccg
     2221 tacgggagtt cgtcgcacgg cgcttccccg gcttgaagga cgcgccgctc gtgggaagcg
     2281 aggtgtgcca gtacgagaac acgtccaatg gcgatttcct gatcgaccgc catcccgagc
     2341 gcgacaacgt gtggctcgtg ggcggcggct cggggcacgg attcaagcac ggtccggcag
     2401 tgggcgagta cgtggcggcg cgcatcgtcg aaggcgggcc ggtcgagccc cgcttcagcc
     2461 tggccaccaa ggagaaggtc cagaagcggg cggtgtactg agagcccggt gaccggcagt
     2521 tcgcggatcg cgcggaacgt cgggccggac gacggcctgc ggtagcatcc gcgcccaacc
     2581 gtgggcgcca gtcggagatc cggagcggtc gggcggaacg tcccgcgcaa agagggcctc
     2641 gacaaggtcg ccggcagggc gaagtacgtc gacgatctct ccttccccgg aatgctccac
     2701 gggcgcacga tccgctcgac ggtgccgcgc ggcgagatca cgggaattcg gctcgacttc
     2761 gatcgggccg ggttcaccat cgccgacttc caggacattc cgggacggaa cacgatcgcg
     2821 ctgatcgagc tcgatcagcc ctgcctcgcc gagcgcgaga tccggcactt cgccgagccg
     2881 atcctgctcc tcgcgcatga caaccgcgac gctctcctcg acgccgagcg ctcgatcacg
     2941 atcgactacc gaaccgccgt gcccgtctac gacccgacgc gctcgcccca gtgcttcaag
     3001 gaaatcgtca tcgagaaggg ggagctcgcg cgcggcttcg cggcggcaga tctcgtcgtc
     3061 gagggtgaat atcgcgccgg ccaccaggag cagctctaca tcgagccgaa cggcgtcatc
     3121 gcggtgcccg aggacggcgg cgtcaccgtt tacggttcgc tccagtgccc gtactacgtc
     3181 caccgcgcct tgaaggtcct gctcggcctg ccggaggaca gagttcgggt cgtgcaggcg
     3241 gagaccggcg gcggattcgg cggtaaggag gagtacccgt ccatgatcgc gggacacgcc
     3301 gcgatcctgg cgctcaaggc cggccggccg gtcaagctcg tctacgaccg cgtcgaggac
     3361 atggtcgcga cgacgaagcg gcacccggcg gccatccgcc accggaccgg cgtcacacgg
     3421 gacggccgcc tgaccgcgat cgagatcgac gtcctcatgg acggcggcgc gtacatgacg
     3481 ctctcccccg tcgttctctc ccgcggtgtc atccacgcaa ccgggccgta ccggtgcgac
     3541 aacgtcctcg tccacggccg cgtcgtgatg acgaacacgc cgccgaatgg ggcgttccgc
     3601 ggcttcggcg cgcctcagac gcagttcgcc gccgaagtcc acatggagcg gatcgccgag
     3661 gcgctcggca tggatccggt ccggctgagg gaagtgaacg cgtatcggcc gggagacacc
     3721 acggcgaccg ggcagaggct cgggcgcgac tgca
//
LOCUS       DASWAV010000357         2315 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U2665
            k141_739180, whole genome shotgun sequence.
ACCESSION   DASWAV010000357 DASWAV010000000
VERSION     DASWAV010000357.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960316
            Sequence Read Archive: SRR10260007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2315)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2315)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:47:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,929
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2315
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_739180"
                     /isolate="SMAG_U2665"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.36444 N 132.31889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            79..555
                     /locus_tag="VF958_07440"
     CDS             79..555
                     /locus_tag="VF958_07440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9148979.1"
                     /translation="MRKNLLMVSLAACCALVLSSSVALAAENWLGTWKLDVAKSKFSP
                     GPGPKSLTLKFETTKNGIKLTSDGVDAGGKATHGEYVSKFDGKDVPWTGNPDADTASA
                     RKIDDNSYENVWKKDGKTTITAKAVVSNKGKTFTTTHTGTDSKGRSVNNTVVYDRQ"
     gene            713..>2315
                     /locus_tag="VF958_07445"
     CDS             713..>2315
                     /locus_tag="VF958_07445"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEX9148980.1"
                     /translation="MPLAAGSKFGVYSILGPLGSGGMGEVYRAFDTRLGREVAVKTLP
                     EEFSDDPERLARFEREARMLAALNHPSIAAIYGLEEAGRARFIVMELVPGETLSEMLS
                     RGPLPFEEALKVARQIADALEAAHERGIIHRDLKPANIKVTPEGRVKVLDLGLAKVFD
                     PKEPASGPDLSVSPTLVSDGTQPGVILGTVEFMSPEQARGKAVDKRTDIWSFGCVLYE
                     LLTGRRVFAGETPTDVLAAILTAEPRWELLPGETPARIRDLLRRSLQKDPNHRLRDIG
                     DARIEIDQALALAPEAFRESPLPTVPLPRPRTSRIRLAAIGAAAVVAALAIVWIARRN
                     FLPPATRSATRFLAVLPFKDLTGRPEGQLLGDAFAEIMSARLASVPDLQVVTPRATDE
                     FSAEEKDFVRVARSTGASLILSGAIQRTGDQLRITCSLVRAPDGVHVGGDEVTTSASE
                     IFGAQDRLAESVFSWLGAKRRAASPARPGTVPLSSAAQETYLKAIGALRRYDVPESIA
                     MAIRILTDLDRKHPDSPLILAALGRA"
BASE COUNT          418 a          787 c          739 g          371 t
ORIGIN      
        1 tatgatccgc ccaccccagc gccgaagtcc cctgacggtt cctgagattc agccgaattt
       61 ttttgaagga ggtagtccat gcggaagaac ctgctcatgg tttccctcgc ggcgtgctgc
      121 gccctcgttc tctcgagttc cgtcgccctc gcggccgaga actggcttgg gacctggaag
      181 ctcgacgtcg cgaaatccaa gttcagcccg ggacccggac cgaaaagcct gaccctgaag
      241 ttcgaaacga cgaagaacgg gatcaaactc acctcggacg gcgtggacgc cggaggaaag
      301 gccacccacg gcgagtacgt gtccaagttc gacggcaagg acgttccctg gacgggcaac
      361 ccggacgccg acacggcttc ggccaggaag atcgacgaca acagttacga gaacgtctgg
      421 aagaaggatg gcaagacgac aatcactgcg aaggctgtcg tgtcgaacaa gggcaagacc
      481 tttaccacca cccacacggg aacggactca aagggtcgaa gcgtgaacaa cacggtggtc
      541 tacgacaggc agtagagcca gcgttccaac caactcccgc gggagtcgac tccacgggga
      601 catgagcccg cgcgtcgcgc gcgggctttt gctttgaagc gtcacgagcc tccggtgact
      661 cccgaaagcg gctcgtcaat cgcgcgagac atagaatccc ggccaactcg caatgcctct
      721 cgcagcgggc tcgaagttcg gggtctattc gattctgggc ccgctcgggt ccggcggcat
      781 gggggaggta taccgcgcct tcgacacccg gctgggtcga gaggtcgcgg tcaagacgct
      841 tcccgaggag ttctccgacg atccggagcg cctggcgcgt ttcgaacgcg aggcccggat
      901 gctcgccgcc ttgaaccacc cctcgatcgc ggcgatctac gggctcgagg aagccggccg
      961 cgctcggttc atcgtgatgg agctggtgcc gggagagacg ctctcggaga tgctttcgcg
     1021 cggcccgctg ccgttcgaag aggccctgaa ggtcgcccgc cagatcgccg acgctctcga
     1081 ggcggcgcac gagcgcggca tcattcaccg ggacctgaaa cccgccaaca tcaaggtcac
     1141 tcccgaaggg cgcgtcaagg tcctcgacct cgggctcgca aaggtcttcg atccgaagga
     1201 acccgcctcc gggccggacc tttcggtttc gccgacgctc gtcagcgacg gcacgcagcc
     1261 gggcgtcatc ctcggcaccg tggagttcat gagtcccgag caggcgcgcg gcaaagccgt
     1321 ggacaagcgg accgacatct ggtcgttcgg ctgcgtgctc tacgagctcc tgacgggccg
     1381 gcgggttttc gccggggaga ccccgacgga cgtcctcgcc gcgatcctca cggccgagcc
     1441 gcgatgggag ctgcttcccg gcgaaacccc cgcacggatc cgagacctcc tccggcgctc
     1501 ccttcaaaag gatccaaacc accggctccg ggacatcggg gacgcgcgga tcgaaatcga
     1561 ccaggcgctg gcgctggcgc cggaggcgtt ccgggaaagt ccgctcccga ccgtacccct
     1621 tcctcggccg cggacctccc gaatacgcct ggcagcaatc ggcgccgcgg cggttgtcgc
     1681 tgcactcgca atcgtctgga tcgcgcgccg gaacttcctg ccccccgcca cccgatccgc
     1741 gacccggttt ctcgcggtcc ttccgttcaa ggacttgacc ggccggccgg aggggcagct
     1801 tctcggcgac gcgttcgccg agataatgag cgcgcgactc gcgagtgtgc ccgacctcca
     1861 ggtcgtcact ccgcgcgcga ctgacgagtt ctccgccgag gagaaggact tcgttcgcgt
     1921 cgcccggtcg acgggagcga gcctcatcct ctcaggcgcc atccagcgaa ccggcgatca
     1981 gcttcggatc acctgttctc tcgtgcgagc gccggacggc gtgcacgtcg ggggagacga
     2041 agtgacgacg tcggcttcgg agatcttcgg ggcgcaggat cgcctggccg agagcgtctt
     2101 ctcctggctc ggcgcaaaaa ggagggcagc atctcccgcg cgcccgggaa cggttccgtt
     2161 gagcagtgcc gcccaggaga cctacctgaa ggcgatcggc gcgctccgcc gctacgacgt
     2221 accggaatcg attgcgatgg ccattcgtat cctgacggat ctggaccgga agcaccccga
     2281 ctcgcccctc attctggccg cgctcggtcg tgctc
//
LOCUS       DASWAV010000358         4304 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U2665
            k141_699257, whole genome shotgun sequence.
ACCESSION   DASWAV010000358 DASWAV010000000
VERSION     DASWAV010000358.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960316
            Sequence Read Archive: SRR10260007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 4304)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4304)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:47:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,929
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4304
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_699257"
                     /isolate="SMAG_U2665"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.36444 N 132.31889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..330)
                     /locus_tag="VF958_07450"
     CDS             complement(<1..330)
                     /locus_tag="VF958_07450"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020722748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003864 - 3-methyl-2-oxobutanoate
                     hydroxymethyltransferase activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HEX9148981.1"
                     /translation="MPTLSDTSPVTIPALAAAKGVEKIAMLTAYDAPSAALLDSAGID
                     VLLVGDSVEMTVYGEENTLSATMDSMVRHSRAVSRAAKRALVVGDMPFLSYQADVSRA
                     VENAGRFL"
     gene            complement(450..1109)
                     /locus_tag="VF958_07455"
     CDS             complement(450..1109)
                     /locus_tag="VF958_07455"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002640717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019136 - deoxynucleoside kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HEX9148982.1"
                     /translation="MPIPSEGDLTLRHIALDGPIGVGKTSLCEMLARRFHGTKVLEDV
                     DNPFLGEFYKKKKGSAFQTQLFFLLSRYQQQREIAQIDLFTTLVVADYHFPKDKIFAY
                     LNLDDSELMIYDRLYSLLSETVPKPDLVIYLQGSLETCMKRIKKASRAIEKGITPEYV
                     AQLIEAYNYYFYHYDETPLLVVDTNEIDFVNRPADFEDLVAQVRRAKKGQQYYVPASS
                     G"
     gene            complement(1124..1996)
                     /locus_tag="VF958_07460"
     CDS             complement(1124..1996)
                     /locus_tag="VF958_07460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014099671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008412 - 4-hydroxybenzoate
                     octaprenyltransferase activity [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiA-like polyprenyltransferase"
                     /protein_id="HEX9148983.1"
                     /translation="MSRAAGVARPLRTTLEMIRFSHTLFALPFAILSAVLAAGGWPPA
                     ATLGKILLAMVGARSAAMAHNRLADRELDAANPRTATRALPAGTLSIDYVRAFLAVSV
                     GIFLLAAASLNRLALLLSPVALAILFFYSYTKRFTSLSHLLLGLCLAIAPVGAWIAVR
                     GELEVLPILLGLAVLFWTAGFDVIYSLQDEDHDRRVGLHSLPARFGARAALAISAIFH
                     AVMVALLVAVWRAAGGGALFAAGIAATAAALVYQHAIVRPGNLSRVNSAFFTANGFVS
                     LVLALCGIADVLTR"
     gene            complement(1993..2637)
                     /locus_tag="VF958_07465"
     CDS             complement(1993..2637)
                     /locus_tag="VF958_07465"
                     /inference="COORDINATES: protein motif:HMM:TIGR00421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0006725 - cellular aromatic compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiX family flavin prenyltransferase"
                     /protein_id="HEX9148984.1"
                     /translation="MADREIVVGISGASGASLPRRFVELAARARGISRLHLVFSESSL
                     IVARNEIDAAIVTPQAWVAALELPREAQRRIELHGNDEVGASIASGSYPVVGMAVIPC
                     SAGTLGAIANGISRDLLQRAADVCLKERRPLVLAFRESPYSLVHVENMRLVTLAGAIV
                     APPTPAFYVSDPSMARFLDAWCARVARLLDIDLPGEDFRWTGTRERPRGSRPAR"
     gene            3034..3507
                     /locus_tag="VF958_07470"
     CDS             3034..3507
                     /locus_tag="VF958_07470"
                     /inference="COORDINATES: protein motif:HMM:NF024245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HEX9148985.1"
                     /translation="MTTRTSPSNPEAGGSIFRYFAAGVLAGAGLLLASSSFGLDTTGA
                     PPALSGKVNINQASVEQIAYLPRIGTKVAARVLDYRKTHGQFARPEDLMEVKGIGEKL
                     FLTLKPYIAVSGPTTLAAKVRLGSSPSRRREAAPPANEKPAPKSAPAAVPSGKGR"
     gene            3504..4136
                     /locus_tag="VF958_07475"
     CDS             3504..4136
                     /locus_tag="VF958_07475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GspH/FimT family pseudopilin"
                     /protein_id="HEX9148986.1"
                     /translation="MTFVPTRDRRRGPGLSRLPAGISSVELVVVMALTAALAAATARG
                     AFQLQSALAVRSAAAELAATFFRARAFAIARGVSVGVKFRKDGGRYEWALYRDGNGNG
                     IRSAEIASGVDRSLGIAVAWQRNDVRPGILEGGSVPDPSTPGQKLDRPDDPIRFGSSD
                     ICSFSPVGESTPGSVYLWDGRDRMAVVRIFGRSAKVRTLYYFRGEKSWRK"
BASE COUNT          768 a         1377 c         1463 g          696 t
ORIGIN      
        1 caggaaacgg ccggcgttct cgacggcgcg ggagacgtcc gcctggtaag aaagaaacgg
       61 catgtcgccg acgacgagcg cgcgtttggc cgcccgcgag acggcgcgcg agtggcggac
      121 catcgagtcc atcgtggcgg agagggtgtt ctcctcgccg taaacggtca tctcgacgga
      181 gtcgccgacg agaaggacgt cgattccggc cgagtcgagg agggcagccg acggggcgtc
      241 gtacgcggtc agcatcgcga tcttctcgac gcctttggcc gcggcgagtg cggggatcgt
      301 gacgggggag gtgtccgaga gagtgggcat gttgcgctcc tttcctctcg gtcgacgtcc
      361 cgaataccgg gatcgccgcc gaaaagggcg tcgggtcaat tatatggggg tcaggtctcc
      421 acttgccaca tcgggggtca ggtctcccat taaccgctgg acgccggaac gtagtactgc
      481 tgccctttct tcgccctccg gacttgcgcg acgaggtctt cgaagtcggc ggggcggttc
      541 acgaagtcga tctcgttggt gtcaacgact aagagcggcg tctcgtcgta gtggtagaag
      601 tagtagttgt aggcctcgat caactgcgcg acgtactccg gcgtgatccc cttctcgatc
      661 gcgcgcgacg ccttcttgat cctcttcatg caggtctcga gggacccctg caggtagatg
      721 acgaggtccg gcttcggaac cgtttcggag agcaacgagt acagccggtc gtagatcatg
      781 agctccgagt cgtcgagatt caggtacgcg aagatcttgt ccttcgggaa gtggtagtcg
      841 gccacgacga gcgtcgtgaa gaggtcgatc tgcgcgatct cccgctgctg ctggtagcgg
      901 gagagaagga agaagagctg cgtctgaaaa gcggagcctt tcttcttctt gtagaactcg
      961 ccgaggaacg ggttgtccac gtcctcgagg accttcgtgc cgtggaagcg ccgggcgagc
     1021 atctcgcaga gcgacgtctt gcccaccccg atagggccat cgagggcgat gtggcgcagc
     1081 gtgaggtcgc cttcggacgg gatcggcacg gccgcgattg taactatctc gtcaggacgt
     1141 cggcgattcc gcacaacgcc agcacgaggg acacgaatcc gttcgccgtg aagaacgccg
     1201 agttcacgcg cgagaggttg ccggggcgca cgatcgcgtg ctggtagacg agggcggccg
     1261 ccgtggcggc gattcccgcc gcgaagagcg cccccccacc cgctgcgcgc cagaccgcga
     1321 cgagaagggc gaccatcacg gcgtggaaga tcgccgagat cgcgagcgcg gcgcgggctc
     1381 cgaagcgcgc cggaagcgag tgcaggccga cgcgccggtc atggtcttcg tcctggagcg
     1441 aatagatcac gtcgaaaccc gccgtccaga agaggacggc gaggccgagg agtatcggaa
     1501 gcacctcgag ctctccccgc accgcgatcc aggcgccgac gggcgcgatt gcgaggcaca
     1561 gtccgaggag gagatgagac agcgacgtga agcgcttcgt gtaggagtag aaaaagagga
     1621 tcgcgagcgc gacgggcgag aggagaagcg cgagccggtt aaggcttgcc gccgcgagga
     1681 gaaagatccc gaccgagacc gccagaaacg cgcggacgta gtcgatcgac agcgtcccgg
     1741 ccggaagggc tcgcgtcgcc gtgcgcggat tcgcggcgtc gagctcccgg tcggcgagcc
     1801 ggttgtgcgc catcgcggcg ctgcgggctc cgaccatcgc aaggagaatc tttccgagcg
     1861 tcgcggcggg cggccagccg ccggcagcga gcacggcgga aagaatcgcg aaaggcagcg
     1921 caaagagcgt gtgcgagaaa cgaatcatct cgagagtcgt gcggaggggc cgggcgactc
     1981 ctgcggcgcg actcatcggg cggggcgcga gccccgtggt cgctcacgag ttcccgtcca
     2041 gcggaaatct tcccccggca gatcgatgtc gagcaggcgc gcgacgcgcg cgcaccacgc
     2101 gtccagaaaa cgcgccatcg aaggatcgct cacgtagaaa gccggcgtgg gaggggccac
     2161 gatcgcaccg gccagggtca cgaggcgcat gttctcgacg tggacgagcg agtacggcga
     2221 ctcgcgaaac gcgaggacga ggggacgacg ttccttcagg cagacgtcgg ccgcgcgctg
     2281 gaggaggtcc cgggaaatcc cgttggcgat cgcgccgagc gttccggcgc tgcacggaat
     2341 gacggccatc ccgacgacgg gataggaacc cgaggcaatc gaggcgccaa cctcgtcgtt
     2401 cccgtgaagc tcaatccggc gctgcgcttc ccggggaagc tcgagagcgg cgacccacgc
     2461 ctgcggtgtg acgattgcgg cgtcgatttc gttgcgggcg acgatcagac tcgattcgga
     2521 aaaaacgagg tggaggcgcg agatcccgcg ggcacgggcc gcgagctcca cgaatcggcg
     2581 gggcagagaa gctcccgaag cccccgagat tccgacgacg atttcgcgat ccgccacggg
     2641 cagagtctat cggcgacggc cggaccttgg cggagcgccg aactccgtca aaagtccggt
     2701 cggcctgcaa gcattctttc cgcccgggcg gcgcctttcc gctcagtgaa ttccgtcgcg
     2761 cccgagctca attccgtcag tttttctttg cgctgcgcgc tcgcctccgc tgacccacgt
     2821 ttcccccaaa ggccttttgg ccgcggcagt tgcggctgag agctgcgcgc ggcacggttc
     2881 tcgcccattt gcctcctcga gaaatcgcag gaggtgccga tggagaacgc ctgaacgcga
     2941 aaactcgatg aggacaccgc tcgagaattg atttgcctac ccactccagc ttctccggtc
     3001 cgcttcccac cactcgaatt gaaggagctc cgaatgacga cccgaacatc tccctcgaat
     3061 cccgaagctg gcggttccat ctttcggtac ttcgctgccg gcgtactcgc cggggcaggt
     3121 ctcctgctcg cttcttcctc tttcggtctc gacacgaccg gcgctccgcc ggccctctcc
     3181 ggcaaggtca acatcaacca ggcttccgtc gaacagattg cctatctgcc gcgaatcggc
     3241 acgaaagtcg cggcaagggt tctcgactat cgaaagaccc atggccagtt cgcgcggccc
     3301 gaggacctca tggaggtcaa aggcatcggc gaaaagctct tcctgaccct gaagccctac
     3361 atcgccgtga gcggccccac cacactcgcc gcaaaggtcc gcctgggctc ttctccttca
     3421 cgaagacgag aagctgcccc tcccgccaac gaaaagcccg ccccgaagtc cgctccggcc
     3481 gccgtcccct cgggcaaggg acggtgacgt tcgtgcccac ccgggaccgt cgtcgcggtc
     3541 ccgggctttc ccgtcttccc gcggggattt catcggtcga gctcgtcgtc gtgatggctc
     3601 tcaccgcggc actcgccgcc gcgacggcgc ggggtgcgtt tcagctgcag tcggcgctcg
     3661 ctgtccgctc cgccgcggca gagctggcgg ccaccttctt tcgcgcacgc gcttttgcga
     3721 tcgcccgcgg ggtgtcggtc ggcgtcaagt tccgcaagga tggcggccgc tacgagtggg
     3781 ccctttaccg cgacgggaac ggaaacggga ttcgaagcgc cgaaatcgcg tcgggcgtcg
     3841 atcggtcgct cgggatcgcc gtcgcgtggc agcgaaacga cgtccggccg ggaatcctcg
     3901 aaggcggctc cgtccccgat ccgagcacgc ccggacagaa gctcgaccgc ccggacgacc
     3961 cgatccgttt cgggagctcc gacatctgtt ctttctcgcc ggttggggag agcacgccgg
     4021 gttccgtcta cctgtgggac ggccgcgacc gaatggcagt ggtacgcatc ttcgggagga
     4081 gcgcgaaggt tcggacgctg tactacttcc gaggcgagaa gagctggagg aagtgagaat
     4141 gtcggctagc cgagtggata gaccgcggcg tcttcgcccg cgcggcccca gagcagtgcg
     4201 tcgcggtggg aggcgccggc cgggatccgg ccggcggccg caatgcagcc gcgcagctcc
     4261 gcaccggcgc cgacttcgac gccgtcccac acggtgcttt cgat
//
LOCUS       DASWAV010000359         2910 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U2665
            k141_258466, whole genome shotgun sequence.
ACCESSION   DASWAV010000359 DASWAV010000000
VERSION     DASWAV010000359.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960316
            Sequence Read Archive: SRR10260007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2910)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2910)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:47:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,929
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2910
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_258466"
                     /isolate="SMAG_U2665"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.36444 N 132.31889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2383
                     /locus_tag="VF958_07480"
     CDS             <1..2383
                     /locus_tag="VF958_07480"
                     /inference="COORDINATES: protein motif:HMM:NF012311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HEX9148987.1"
                     /translation="IFASPVLAAQADPSEVAARTARAADGNLVVLVELVDPPAARYAG
                     GIAGLAPTAPAKTGISLLAAAHREDVLRYEEYLDARREEVIGRLRARIPEAEVGAKYR
                     TVWNGFAVSIPAGSVRDLAELPGVAAIHRVREYSPTLDASNPAMGAPAFWSSLGGDDR
                     AGAGMKVGIIDTGVDFSNPMFSDPTLTPPPGFPKGDPTLANGKVIVAKFFQSAADAND
                     ANANLGHRTAQDLVGHGSHSAGVAAGSRVDLAGSGRRAITVAGVAPKAWIGNYRVFAP
                     RAFDDNIIAAIEEAVQDGMDVINMSFGGDPVGDPLRDPIIRATQNAIAAGVVATISAG
                     NAGPDPSTVAYPGAAPDAITVGATSNAHYGLSSLGILSSAAGSTVPGSLASITGGACA
                     DRCALPASPASAAMLDVDLVDGQLSGIACTALPGGSLNGRIALVQRGTCTFAVKGQNV
                     QAAGAVGMVISNSDDPAVSNSGEQIFNPGLSPASVPALLVRRSDGLALKSFLDGGPTA
                     ESTIWMLGAAPPGTPPFVGAATPDRLTSFSSRGPTPTLAIKPDLATVGADSYAPFQDD
                     SANGENRFPAPDPHRGQSALFDASGFGFAAGTSFSAPRTAGAAALVKQKHPGWTPDLV
                     KAALTETASRPADSSRIGSERVMARVAGDIDLAAAATVESLVLPTSTSFGRVTTESAP
                     FTLTKTFTLKNLSSAGVTYSLEAARSAGDAFITTNVEPASIALGPGEEGSVTLRVTVG
                     SGVAASEIDSEGFLAVSDGRMTIPEILYVPWWIRTVPLTPSEPYLRAINARGK"
     gene            complement(2364..2606)
                     /locus_tag="VF958_07485"
     CDS             complement(2364..2606)
                     /locus_tag="VF958_07485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9148988.1"
                     /translation="MARTSGATKRSARGGETPRQSFLVRDCYTVSGLVVGDICFTCRC
                     RVVEFAGEDGMTLAWCDCEWADDAAEMEVLTTFPER"
     gene            complement(2611..>2910)
                     /locus_tag="VF958_07490"
     CDS             complement(2611..>2910)
                     /locus_tag="VF958_07490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9148989.1"
                     /translation="KYSMDHETVEEIKRHFNVVAEGLRSDIRLVIEGLAANTDRLDGV
                     DVRLDGIDVRLDRLETGFDGFESRVAAEFTETRAMVRLSYGELERRIERFEGRGG"
BASE COUNT          452 a         1055 c          926 g          477 t
ORIGIN      
        1 cattttcgcg tcgccggtcc tcgcggcgca ggcggacccc tcggaggtcg ccgcgaggac
       61 cgcgcgcgcg gcggacggga acctcgtggt gctcgtcgag ctcgtcgatc ccccggccgc
      121 gcgctacgcc ggtggaatcg ccgggctggc cccgactgct cccgccaaaa ccggcatttc
      181 gctgctcgct gccgcgcacc gggaggacgt cctgcgatac gaggagtatc tcgatgcccg
      241 ccgggaggaa gtcatcggtc gcctgcgcgc gcgaattccg gaggcggagg tcggcgcaaa
      301 gtatcgaacc gtgtggaacg gcttcgccgt ctcgattcct gcaggctcgg tgcgcgatct
      361 ggcggagctt cccggcgttg cggcgatcca ccgggtccgc gagtactctc cgaccctcga
      421 cgcttcgaat cccgccatgg gcgctcccgc gttctggtcg tctctcggcg gagacgatcg
      481 cgcgggcgcg gggatgaagg tcgggatcat cgacacggga gtcgatttct ccaaccccat
      541 gttttccgac ccgactctca caccgccccc cggttttccg aaaggcgacc cgactctcgc
      601 gaacggaaag gtgatcgtcg cgaagttttt ccagtcggcg gccgacgcga acgacgcgaa
      661 cgcgaacctc ggccaccgca ccgcccagga cctcgtcggg cacggctccc actcggccgg
      721 ggtcgccgcg gggtcccgcg tcgacctcgc cggctcgggg cgtcgcgcga tcacggtcgc
      781 gggcgtcgcg ccgaaggcat ggatcggcaa ctatcgcgtc ttcgcgccgc gcgctttcga
      841 cgacaacatc atcgcggcga tcgaggaagc ggtgcaggac gggatggacg tcatcaacat
      901 gagtttcgga ggggatccgg tcggggaccc gctgcgcgat cccataatcc gcgcgactca
      961 gaacgcgatc gcggccgggg tcgtcgcgac gatcagcgcc ggcaacgccg gaccggaccc
     1021 gtccaccgtc gcctatcccg gtgcggcacc ggatgcgatc accgtcggcg cgacgtcgaa
     1081 tgcgcactac gggctctcgt cgctcggcat tctctcgtcc gccgccggct cgacggtccc
     1141 cggcagcctc gcctcgatca ccggcggggc ttgcgccgac cgctgcgcgc ttcctgcctc
     1201 gccggcgtcg gccgcgatgc tcgacgtcga cctcgtcgac gggcagctct cggggatcgc
     1261 gtgcaccgct ctccccggcg gctcgttgaa cggccggatc gcgctcgtcc agcgcggcac
     1321 ctgcacgttc gccgtcaagg gacagaacgt gcaagccgca ggcgcggtcg gcatggtgat
     1381 ctccaactcg gacgacccgg ccgtctcgaa cagcggtgag cagattttca atccgggcct
     1441 ctcgccggcg tccgtgcctg cgttgctcgt ccgccgcagc gacggcctcg cgctcaagtc
     1501 gtttctcgac ggcggtccga ccgccgaatc gaccatctgg atgctcggcg ccgccccgcc
     1561 gggaacgccg ccgttcgtcg gcgcggcgac gccggaccgt ctgacgtcgt tcagttcccg
     1621 cgggcccacc ccaacgctcg cgatcaagcc ggacctcgcc accgtcggcg ccgacagcta
     1681 tgcgccgttc caggacgatt cggcgaacgg cgaaaaccgt ttccccgctc cggacccgca
     1741 ccgcggtcag agcgcgcttt tcgacgccag cgggttcggg ttcgctgctg gaacctcctt
     1801 ctcggctccc cgcaccgccg gcgccgcggc tctcgtcaag cagaagcacc cgggttggac
     1861 gcccgatctc gtcaaggccg cgctcacgga aaccgcgagc cgtccggccg attcaagccg
     1921 gatcggttcc gagcgcgtca tggcgcgagt ggcgggcgat atcgacctcg ccgcggcagc
     1981 aaccgtcgag agcctggtcc tcccgacgag cacgagcttc ggacgcgtga cgaccgaatc
     2041 cgcgcccttc acgctgacga agacgttcac gttgaagaac ctctcgagcg ccggcgtgac
     2101 ctattcgctc gaggcggcgc ggtcggcggg agacgcgttc atcaccacga acgtcgaacc
     2161 cgcaagtatc gctctcggtc cgggcgaaga gggaagcgtc accttgcgcg tgaccgtcgg
     2221 gagcggcgtc gccgcgtccg agatcgactc cgagggtttt ctcgccgtgt ccgatggacg
     2281 gatgacgatc cccgagatcc tctacgtccc gtggtggatc cggaccgtgc cgttgacgcc
     2341 gtcggagccg tacttgagag cgatcaacgc tcggggaaag tagttagaac ctccatctcc
     2401 gcggcgtcgt cggcccactc gcaatcgcac cacgccagcg tcatcccgtc ttccccggcg
     2461 aactccacga cccggcagcg gcacgtgaag cagatgtccc cgacgaccag gccggaaacc
     2521 gtgtagcaat cgcgaaccag aaagctctgt cgaggcgtct caccgcctct cgccgaccgt
     2581 ttcgtagcgc cacttgttcg ggccacgctc tcaacctccc cgtccttcga agcgttcgat
     2641 tcggcgctcg agctcaccat aagacagccg aaccattgcc cgggtttccg taaactcagc
     2701 ggccacgcgg ctttcaaacc cgtcgaagcc cgtctcgagg cggtcgagcc ggacgtcgat
     2761 accatcgagc cggacgtcaa caccatcgag tcggtccgtg ttcgcagcca ggccttcgat
     2821 gacgagacgg atgtccgaac gcagcccttc ggccacgacg ttgaaatgcc tcttgatctc
     2881 ttcgaccgtt tcgtggtcca tcgaatactt
//
LOCUS       DASWAV010000360         2921 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U2665
            k141_20233, whole genome shotgun sequence.
ACCESSION   DASWAV010000360 DASWAV010000000
VERSION     DASWAV010000360.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960316
            Sequence Read Archive: SRR10260007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2921)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2921)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:47:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,929
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2921
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_20233"
                     /isolate="SMAG_U2665"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.36444 N 132.31889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..648)
                     /locus_tag="VF958_07495"
     CDS             complement(<1..648)
                     /locus_tag="VF958_07495"
                     /inference="COORDINATES: protein motif:HMM:NF024923.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoyl-binding protein"
                     /protein_id="HEX9148990.1"
                     /translation="MRKRTKIILAVVGALVLLFVAMGFIKRKDKDVARVTTAKVEKID
                     LISKVTANGKIQARRKVDLSALVMGQIVNLAVKEGDKVNRGQLLLQIDRAQLAAQNAG
                     RESAVAAMRSDLQAARANAEQSRLDYERARRNWEAKILSEAEYQRAKSVYDTAQANLS
                     ATQQRLQESGANLAASRDSLSKTTVSAPIAGVVTYLPVKEGEVTVIGTMNNPGTQL"
     gene            complement(792..868)
                     /locus_tag="VF958_07500"
     tRNA            complement(792..868)
                     /locus_tag="VF958_07500"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(832..834),aa:Arg,seq:acg)
     gene            861..1415
                     /gene="tadA"
                     /locus_tag="VF958_07505"
     CDS             861..1415
                     /gene="tadA"
                     /locus_tag="VF958_07505"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008522735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008251 - tRNA-specific adenosine
                     deaminase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0002100 - tRNA wobble adenosine to inosine
                     editing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HEX9148991.1"
                     /translation="MGAGAAYITHNSPSFQAIPARGEPTALRYPAAVAFTPDDEKFLR
                     EAIAFAKIAFSEREVPVGAVVVRDGHMIGRGRNRREALADPTHHAEIEAIREAAQAAG
                     TWRLEGATLYATVEPCPMCAGAAVNARIARIVYGCADPKAGYCGTLGNIPQDSRLNHR
                     CAVEGGLLAEECGDLMKLFFRARR"
     gene            1458..1547
                     /locus_tag="VF958_07510"
     tRNA            1458..1547
                     /locus_tag="VF958_07510"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1492..1494,aa:Ser,seq:cga)
     gene            1597..2523
                     /locus_tag="VF958_07515"
     CDS             1597..2523
                     /locus_tag="VF958_07515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020258929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX9148992.1"
                     /translation="MTLAIELENVSKFFGKFAAVDSLSFRVSRGVVYGLLGPNGAGKT
                     TTIRMIMNITAADSGEITVLGQPMTRETQNRIGYLPEERGLYRKMKVIDHLLFLAAIK
                     EVSREDARRRIADWSDKLELKPWLKKKVDELSKGMQQKVQFIATVIHDPEILILDEPF
                     SGLDPINTAIIKDYLTEFRKKGKTIVFSTHVLEQAEKLCDEICLLARSKKVLEGNLRE
                     LKHRFSKNLLHLSIDARPAEIAALPGVASSQSVNGGYVVTLAPGTDQREFLRHALERY
                     PLSGFSTKEPELEEIYLDAIRAAGIEETRTIE"
     gene            2531..>2921
                     /locus_tag="VF958_07520"
     CDS             2531..>2921
                     /locus_tag="VF958_07520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9148993.1"
                     /translation="MSWTKIFAVIRREYLERVRTKAFWIATFLVPIFFLGYMAVQFAA
                     IKKTSGERKVAVVDTTGRLAPALAAELAAREDALHRSKPGSRGIHWIVESRPITGDLE
                     RTKETLRKEVLEKKIYGYLGLDPALLEK"
BASE COUNT          542 a          955 c          910 g          514 t
ORIGIN      
        1 cagctgcgtc cccggattgt tcatcgtccc gatgaccgtg acctcgccct ccttgaccgg
       61 aaggtaggtg acgacgccgg cgatcggcgc gctcacggtc gttttcgaga gggagtcgcg
      121 actcgcagcg aggttcgcgc cagactcctg aaggcgctgc tgggtcgccg agagattcgc
      181 ctgagcggtg tcgtagacgg atttcgccct ctgatactcc gcctcggaaa ggatcttcgc
      241 ttcccagttg cgccgcgccc gctcgtagtc gagccgcgac tgttccgcgt tcgcccgcgc
      301 cgcctggaga tccgagcgca tcgcagcaac cgcggactcc cgccccgcgt tctgcgcggc
      361 gagctgtgcc cgatcgatct gaaggagcaa ctgcccgcgg ttgaccttgt cgccctcctt
      421 gacggccagg ttgacgatct gacccatgac gagagcggag aggtcgactt tccggcgcgc
      481 ctggatcttg ccgttggccg tgaccttgga gatgaggtca atcttctcga ccttggcggt
      541 cgtcactcgc gcgacgtcct tgtccttcct cttgatgaat cccatggcga cgaacaggag
      601 caccagggcc ccgacgaccg ccagaatgat cttcgttctc ttcctcatct ccctcctcca
      661 gtgacacgag ctgaaacgtc tattttctta cggccgaagg gctcaaaagt tcgcccgggc
      721 ggcgtggccg tcaggtccgc cagcactgcg gcgggtgcag acccgcaggg cctgtcccgg
      781 cgatgttcga gtggtgcgcc caggaggatt tgaaccccca acctcctgat ccgtagtcag
      841 acgctctatc caattgagct atgggcgcag gagcggcgta cattactcac aacagcccct
      901 cttttcaagc aattccggcg cgcggggagc ccaccgcgct gcgataccct gccgccgtgg
      961 cgttcactcc cgacgacgag aagttcctgc gggaagcgat cgcgttcgcg aagatcgcct
     1021 tctccgagcg agaggttccg gtcggcgccg tcgtggtccg ggacgggcac atgatcggcc
     1081 gcgggcgaaa ccggcgcgag gcgctcgccg acccgaccca ccacgccgag atcgaggcga
     1141 tccgggaagc ggcgcaagcg gcggggacct ggcggctcga aggcgcgacg ctgtacgcga
     1201 ccgtcgagcc gtgcccgatg tgcgctggcg cggcggtcaa tgcacggatc gcccggatcg
     1261 tgtacggctg cgcggacccc aaggcgggct actgcgggac cctcgggaac atcccgcagg
     1321 actcgcgcct gaaccaccgg tgcgccgtcg agggcggtct cctcgccgag gaatgcggag
     1381 acctcatgaa gctctttttc cgggctcggc gatagcgtcg aacccccaag ttcgctagga
     1441 tttcgcgttt cgcgcacgga gaggtgctgg agtggttgaa caggccggtc tcgaaaaccg
     1501 gtaagcccta acgggcttcg agggttcgaa tccctccctc tccgccacag agccatcgaa
     1561 acttgggacg gtttcacccc gtaaagacag ggacacatga ctctcgccat cgagctcgag
     1621 aacgtctcga aattcttcgg caagttcgct gccgtggact cgctgtcctt tcgggtctcg
     1681 cgcggtgtcg tctacgggct cctcggtccc aacggcgcgg gcaagacgac cacgatccga
     1741 atgatcatga acatcaccgc ggccgattcg ggagagatta ccgtcctcgg gcagcccatg
     1801 acccgcgaga cccagaaccg aatcgggtac ctgcccgaag agcgtggtct ctaccggaag
     1861 atgaaggtca tcgaccacct cctctttctc gctgcgatca aggaggtttc gcgtgaagac
     1921 gcgcggcgac gcatcgcgga ctggtcggac aagctcgaac tcaaaccatg gctcaagaag
     1981 aaggtcgacg agctgtccaa gggcatgcag cagaaggtcc aattcatcgc cacggtcatt
     2041 cacgatcccg agatcctcat cctggacgag ccgttctcgg gactcgaccc gatcaacacg
     2101 gcgatcatca aggactacct gacggagttc cgcaagaagg ggaagacgat cgtcttctcc
     2161 acccacgtgc tcgaacaggc ggagaagctc tgcgacgaga tctgcctgct cgcgcgatcg
     2221 aagaaggtcc tcgaaggaaa cctgcgcgag ctgaagcacc gcttttcgaa gaacctgctc
     2281 cacctgtcga tcgacgcccg tcccgccgaa atcgcggctc tcccgggggt cgcgtcgtcc
     2341 cagtcggtga atggagggta cgtcgtcacg ctggcacccg ggacggacca gcgcgagttc
     2401 cttcgccacg cgctcgagcg ttaccccttg agcggcttct cgacgaagga gccggagctc
     2461 gaggagatct acctcgacgc aattcgcgcg gcggggatcg aagagacccg cacgatcgag
     2521 tgaggaagcc atgagctgga ccaagatctt tgccgtcatt cgccgggagt acctggagcg
     2581 tgtccggacg aaggcgttct ggatcgcgac ctttctcgtt ccgatcttct tcctcggcta
     2641 catggcggtt cagttcgcgg caatcaagaa gacgagcggc gaacggaagg tcgccgtcgt
     2701 cgacacgacg ggacggctcg ccccggccct cgccgccgag ctcgcggcgc gcgaagacgc
     2761 gctgcaccgc tccaaaccgg gctcgcgcgg gatccactgg atcgtcgagt cacgtccgat
     2821 cacgggcgat ctcgaacgca cgaaggagac gctgcggaag gaagtcctcg aaaagaagat
     2881 ctacggctat ctcggtctcg acccggcgct cctcgaaaag g
//
LOCUS       DASWAV010000361         6456 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U2665
            k141_699341, whole genome shotgun sequence.
ACCESSION   DASWAV010000361 DASWAV010000000
VERSION     DASWAV010000361.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960316
            Sequence Read Archive: SRR10260007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 6456)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6456)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:47:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,929
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6456
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_699341"
                     /isolate="SMAG_U2665"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.36444 N 132.31889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1921
                     /locus_tag="VF958_07525"
     CDS             <1..1921
                     /locus_tag="VF958_07525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="excinuclease ABC subunit A"
                     /protein_id="HEX9148994.1"
                     /translation="DLFRACRRYSVRTDVPIARLSAHEREVLIKGRGEFYGINGFFEW
                     LETKRYKIHVRVLLARYRAYRLCPDCNGARLGTASMWVRFRGRTIADLAELSLRDLKK
                     WFDELSLSLEEEERLGSVVAEIRSRVRYMNDVGLEYLTLSRAARTLSGGESQRIGLAS
                     ALGGSLTGTLYVLDEPTIGLHAVDTRRLLAILRRLADRGNTVVVVEHDPEAIEAADHV
                     IDLGPGAGSRGGELLFEGTPHALARVKSSATGALLRRRGLPGRGGAGAPDTRIGPPRE
                     ARGGRVVAFRRAEGAVTVVGAREHNLANLTVRFPLGRLVSVSGVSGSGKSTLVRDVLH
                     NAYARRFKGTVQLDVGRHDRIEGLDLISDILLVDQSPLGRSARSNPVTYTKAWDEIRQ
                     VFARNARAVSRRITARDFSFNSAGGRCEICKGTGWQTIDMQFLADVTVRCDACDGRRF
                     QGRVLAVRHRGKNIGDVLALTVDEAMQFFSDEPAIVRRLTPLHEAGLGYLPLGQPTAT
                     LSGGEAQRLKLASFLEIRPATSRGVLFLFDEPTTGLHASDVDRLLGTFRRLISRGHSV
                     IAVEHHLEFLATSDWIIDLGPGGGDEGGRIVAEGTPDEIRRSPASLTGRFLAEGKTSP
                     PPEERSERSRAPRSS"
     gene            complement(1928..2491)
                     /locus_tag="VF958_07530"
     CDS             complement(1928..2491)
                     /locus_tag="VF958_07530"
                     /inference="COORDINATES: protein motif:HMM:NF015168.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HEX9148995.1"
                     /translation="MPEVIPAVAASSEAPVRATTPGGSQSKLSSILFLVLIAAALFRI
                     VTAVTDKGSGGSTGVGLIRWAEAESAGATARASGKPLLYDFTAEWCGPCRMLDADGWQ
                     DPGVAALVNESYVPARVVDRMREEGKNPLWIDELQRKYDVNAFPTLVVASPDGRQIAV
                     SQGYAGKQKLVEFLEVSRKAQPSAATR"
     gene            complement(2493..3194)
                     /locus_tag="VF958_07535"
     CDS             complement(2493..3194)
                     /locus_tag="VF958_07535"
                     /inference="COORDINATES: protein motif:HMM:NF024741.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-2 transporter permease"
                     /protein_id="HEX9148996.1"
                     /translation="MVRRFVRQKFGSGGLVAALVVLGLLEVLGLASHATQRGFVMASW
                     AIFILAAGSVSRDVSSGALQMILSRPIRRTQYLFGRYVGILTSYAIFLAATSVLALVV
                     QFLLPRATRAALPEFPPSALAAAAGAAFLSGALQAAILLFFSTFLPGIADLLGLLLLY
                     LVLNLPQLSQAPWLRTAAEAARENMLPAVSWESVLRGDGGLGAATGRWVFALTVYLAL
                     AAVVFSRRELPYGQE"
     gene            complement(3200..4087)
                     /locus_tag="VF958_07540"
     CDS             complement(3200..4087)
                     /locus_tag="VF958_07540"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX9148997.1"
                     /translation="MSKTYGRFGRRVRALDGVSLNVARGETLGLIGPNGAGKTTLLTC
                     LLGFLRPDRGSITVDGRVPDDLAVRARTGYLPERLGFDRELPGRAFLGFHWRLAGGDP
                     SMADREIAAIADRVGLEREALDRRLRGYSRGMLQRIGLAQALLRDPELLFLDEPASGT
                     DPRGVALVRERILEAKARGATIVLNSHQLPEVERVCDRIVFIDQGRLTKSETLKGASA
                     TRRRGLVRVPASRVVEAASVLAAEAVGADAEGDGTLRFAVGSDDDMARAIRALALADI
                     PVHEARVSAELEELFETKS"
     gene            4173..5204
                     /locus_tag="VF958_07545"
     CDS             4173..5204
                     /locus_tag="VF958_07545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014067289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX9148998.1"
                     /translation="MRALFLTVGMIEVEHLTKRFPTQLAVSDVSFHVRAGEIVGFLGP
                     NGAGKTTAMRILTGFLPPTAGTARIAGFDVVDQSQKARARLGYLPESAALYPEMRVRE
                     YLAYRARLEGVRGPDVRSRVDEAIESCLLDEVAGRKVENLSRGYRQRTALAGALVHKP
                     AVLILDEPTVGLDPAQIIRIRETIRRLGRERAVLLSTHILPEVDAVCDRVLIIDRGCI
                     VAEGTAAELRTRLAGAPVVRASFAGQVAAREVLSALPGVTAVSEENVDAETRVRLECE
                     AGADPREEVFRAAVARGWTLRELLRESMSLEDVFVRLTRHEEATPEGASAASETLDEV
                     GAARGVEPS"
     gene            5201..5941
                     /locus_tag="VF958_07550"
     CDS             5201..5941
                     /locus_tag="VF958_07550"
                     /inference="COORDINATES: protein motif:HMM:NF013245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX9148999.1"
                     /translation="MSKFFAIVGREWRAYFLSPLAYVILAAFLLMNGMIFSAIVAFLN
                     TPGAPKGQALPFLFTNTYFWIFNLFVVPIITMRLFAEERKTGTFETLLTSPVSETTVV
                     LGKFTGALGFFLTLWAPTLIYVWILRSQTPIDLGPVAAGYLAITMIGSYFLAIGIFAS
                     TLTKNQIVAAILAFAMLIPVFSAGLLETLSNDPKKTETLGYLNLWDHMEEFSRGVVDT
                     RRIVYYLSATVFFLYLTVVSLSAKKENP"
     gene            5938..>6456
                     /locus_tag="VF958_07555"
     CDS             5938..>6456
                     /locus_tag="VF958_07555"
                     /inference="COORDINATES: protein motif:HMM:NF021351.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gldg family protein"
                     /protein_id="HEX9149000.1"
                     /translation="MNRGRAARAGTSAAAIALGVALLLGVNWLGSRHWARGDWTKTRI
                     YSLSSTTRKLIGGLSKPVRITVFMDRDARLWTPVSELLARYRALSPKIEVEYLDPKRN
                     PARAEALVREFGIRQSTVVFRSGDRKKYVEEDKLADFDFANAQMGRSAPEIKAFKGEE
                     AFTSAILAVTEAR"
BASE COUNT         1045 a         2189 c         2203 g         1019 t
ORIGIN      
        1 cgacctcttc cgcgcctgcc gccgctactc cgtgcgcacc gacgttccga tcgcacgact
       61 ttccgcgcac gagcgggagg tcctcatcaa ggggcgcggc gagttctacg gaatcaacgg
      121 cttcttcgag tggctcgaga ccaagcggta caagatccac gtccgcgtgc tcctcgcacg
      181 gtatcgcgcc tatcggctct gtcccgactg caatggtgcg cgcctgggca ctgcatcgat
      241 gtgggtgcgc ttccggggcc ggacgatcgc ggacctcgcc gagctttcgc tcagggatct
      301 gaagaagtgg ttcgacgagc tttcgctctc gctggaagag gaggagcggc tcgggtcagt
      361 cgtggcggag atccgctcgc gggtgcgcta catgaacgac gtgggcctcg agtacctgac
      421 gctctcgagg gccgcccgga cgctctcggg cggagaatcg cagcgcatcg gcctcgcctc
      481 ggccctcgga gggtcgctga ccgggacgct ctacgttctc gacgagccga cgatcggcct
      541 ccacgcggtc gacacgcgcc gcctcctggc gatcctgcgt cgcctcgccg accggggcaa
      601 cacggtcgtc gtcgtcgagc acgatcccga ggcgatcgaa gcggcggatc acgtaatcga
      661 cctcggtccc ggcgcgggga gtcggggcgg ggagctgctc ttcgaaggaa caccgcacgc
      721 gctcgcccgc gtcaagtcct ccgcgaccgg cgcactcctg cgccggcgcg ggctgccggg
      781 aagaggggga gcgggagcgc ccgatacgag gatcgggccg ccgcgcgagg cgcggggcgg
      841 ccgcgtcgtt gccttccggc gagccgaggg cgccgtcacg gtcgtcggcg cgagagagca
      901 caacctggcg aacctgaccg tgcgttttcc gctcgggcgc ctcgtttcgg tttccggggt
      961 ctccggttcg ggaaagtcga cgctcgttcg cgacgtcctc cacaacgctt acgcgaggcg
     1021 cttcaaaggg accgtgcagc tcgacgtcgg gcgacacgat cggatcgagg ggctcgatct
     1081 catctcggac atcctgctcg tcgaccagag cccgctcggc cgctcggcgc gatcgaatcc
     1141 cgtgacctac acgaaggcct gggacgagat ccgccaggta ttcgcccgga atgcgagggc
     1201 agtctcgcgc cggatcaccg cgcgcgattt ctccttcaac tccgcggggg ggcgctgtga
     1261 gatctgcaag ggaacgggct ggcagacgat cgacatgcag tttctcgccg acgtgacggt
     1321 gcgctgcgac gcgtgcgacg gtcgccgatt ccaggggcgc gtgctcgcgg tgcgtcaccg
     1381 cggaaagaac attggggacg tgctcgcgtt gaccgtcgac gaagcgatgc agttcttctc
     1441 ggacgagccg gcgatcgtcc gccggctgac gccgctccac gaggcggggc tcggctatct
     1501 ccctctcggc cagcccaccg cgaccctctc gggcggcgag gcccagcggc tgaagctcgc
     1561 ttcgttcctc gagatccgcc ccgcgacttc gcgaggcgtg ctgttcctct tcgacgagcc
     1621 gacgacggga ctccacgcct ccgacgtcga ccgcctcctc ggcacattcc gccggctgat
     1681 ctcgcgcggc cactcagtta tcgccgtgga gcaccacctg gaattcctcg ccacttccga
     1741 ctggatcatc gacctcggcc cgggaggggg agacgaaggt ggccgcatcg tggcggaagg
     1801 tacgcccgac gagatccgcc gatcgcccgc cagcctgacc ggccgattcc tggcggaggg
     1861 gaagacatct ccgccgccgg aagagcgcag cgaacgctcg cgggctccgc gctcgagctg
     1921 agagctctca gcgcgtggcg gccgagggct gcgccttgcg cgagacctcg aggaactcga
     1981 ccaacttctg ctttccggcg tagccctgcg aaacggcgat ctgccgcccg tcgggggacg
     2041 cgacgacgag ggtcgggaac gcgttgacgt cgtacttgcg ctgcagttcg tcgatccaga
     2101 ggggattctt tccctcctcg cgcatgcggt cgacgacgcg cgccggtacg taggattcgt
     2161 tgacgagcgc ggcgactccg ggatcctgcc agccatcggc gtcgagcatc cggcaggggc
     2221 cgcaccactc ggccgtgaag tcgtagagga gcggcttccc ggaggcgcgg gcagtcgctc
     2281 ccgccgactc cgcctcggcc caccggatga gaccgacgcc cgtcgaaccg cccgaaccct
     2341 tgtccgtcac cgccgtgacg attcggaaga gagccgccgc gatcaggacc aggaacagga
     2401 tcgacgagag tttcgactgg gaacctcccg gagtcgtcgc ccgcaccggc gcttcggaag
     2461 aagccgccac cgcgggaatc acttcgggca tgtcactcct gtccataggg cagctccctc
     2521 cgactgaaca cgacggccgc cagcgccaga tacaccgtca acgcgaaaac ccagcggccg
     2581 gtcgccgcgc cgagcccgcc atcaccgcgc agaaccgact cccaggaaac cgcgggcaac
     2641 atgttctcac gggctgcttc tgcggcggtc cgcagccacg gcgcctggga gagctgggga
     2701 aggttcagga ccagatagag gagaaggagg ccgaggaggt ccgcgatgcc cggcaggaag
     2761 gtcgagaaga agagcaggat cgcggcctga agcgctccgg agagaaacgc cgcgcccgca
     2821 gccgccgcga gcgcactcgg cggaaattcc ggtaaggcgg cgcgtgtcgc gcggggcagg
     2881 aggaattgga cgaccagagc gaggacgctc gtcgcggcga ggaagatcgc gtacgacgtg
     2941 aggatcccga cgtagcggcc gaagagatac tgggtccggc ggatcgggcg ggagagaatc
     3001 atctggagcg cgccgctcga cacgtcccga gacacggagc ccgcggcgag aatgaagatc
     3061 gcccagctcg ccatcacgaa gccgcgctgc gtcgcgtgag aagcgaggcc cagcacctcg
     3121 aggagcccga gcacgacgag cgcggcaacg agccctcccg aaccgaactt ctgccggacg
     3181 aagcggcgca ccatctcgat cacgacttcg tctcgaagag ctcctcgagc tccgccgaca
     3241 cccgagcctc gtggaccgga atgtcagcca gcgcaagggc gcggattgct cgtgccatgt
     3301 cgtcgtcgga accgaccgca aaccggaggg tcccgtctcc ctccgcgtcc gcaccgaccg
     3361 cctctgcggc cagaacggat gccgcctcga cgacgcggga cgccgggacg cgcacgaggc
     3421 cgcggcggcg ggttgccgat gcgcccttga gcgtttcgct cttcgtcagg cgtccctggt
     3481 cgatgaagac gatgcggtcg cacacccttt cgacttccgg gagctggtgg gagttcagaa
     3541 cgatcgtcgc tccccgtgcc ttcgcctcca ggatcctttc gcgcacgagc gcgacgccgc
     3601 ggggatcggt tccggacgcg ggctcgtcca ggaaaaggag ttccgggtcg cgaagcaggg
     3661 cctgggcgag gccgatgcgc tgcagcatcc ctcgcgagta gccgcgcagg cggcggtcca
     3721 gggcttcgcg ctcgagcccg acgcggtcgg cgatggccgc gatctcgcgg tccgccatcg
     3781 agggatcgcc ccccgcgagg cgccagtgga agccgagaaa ggcgcgaccc gggagctctc
     3841 gatcgaaccc gagccgctcg ggcaggtagc ccgtgcgcgc gcgcacggcg aggtcgtcgg
     3901 gaacgcgtcc gtccacggta atcgagccgc gatcggggcg cagaaatccc agcaggcagg
     3961 tgaggagggt cgtctttccg gcgccgttcg ggccgatcaa tccgagcgtc tcgccgcgcg
     4021 cgacgttcag gctcacgccg tcgagcgctc ggacccggcg gccaaagcgg ccgtacgtct
     4081 tactcacctc ctgaaccgag atcatgcgcg gttcatctta gcgaagaggg aggcggcgtc
     4141 cgcgcgcggc ttttgcggga atacatgcta gattgcgcgc gctttttttg accgttggaa
     4201 tgatcgaagt cgagcatctc accaagcgct tcccgacaca gctcgctgtg tcggatgtct
     4261 cgtttcacgt gcgcgcgggg gagatcgtcg ggtttctcgg gccgaacggc gccggcaaga
     4321 ccaccgcgat gcggatcctg acggggttcc tgccgccgac cgcggggacc gcgcgcatcg
     4381 cggggttcga cgtcgtcgac caatcgcaga aggcgcgcgc ccgcctcggg tacctccccg
     4441 agtccgccgc gctctacccc gagatgcgcg ttcgagagta cctcgcttat cgcgcgcgtc
     4501 tcgaaggagt tcgcggaccg gacgtccgga gccgggtgga cgaagcgatc gagagctgcc
     4561 tgctcgacga ggtcgccggc cgcaaagtgg agaacctgtc ccgcgggtat cggcagcgca
     4621 cggcgctcgc cggcgccctc gttcacaagc ccgcggtcct gatcctggac gagccaaccg
     4681 tgggcctcga tcccgcgcag atcatccgca tccgcgagac gatccggcgg ctcggacgcg
     4741 agcgcgccgt gcttctgtcg acgcacatcc ttccggaggt cgacgcggtg tgcgaccgcg
     4801 tgctgatcat cgatcgcgga tgcatcgtcg cggaggggac ggccgccgag ctccgaacgc
     4861 ggctggcggg ggcgcccgtc gtgcgagcgt cctttgccgg ccaggtcgcg gcgcgcgaag
     4921 tgctgtccgc gctcccgggg gtcacggcgg tctcggaaga gaacgtggac gccgaaacgc
     4981 gcgtccggct cgagtgcgaa gcgggcgccg acccacgcga agaggtcttc cgcgccgcgg
     5041 tcgcgcgggg ctggaccctg cgggagctcc tgcgcgagtc gatgtcgctc gaagacgtct
     5101 tcgtccggct gacccgccac gaggaggcaa cgccggaggg cgcgagcgcg gcttcggaaa
     5161 cgttggacga ggtcggtgcg gcgcgggggg tagagccgtc gtgagcaagt tcttcgcgat
     5221 cgtcgggcgc gagtggcgcg cctacttcct ttcgccgctc gcgtacgtca tcctcgccgc
     5281 tttcctgctg atgaacggga tgatcttttc ggcgatcgtc gcgttcctga acacgcccgg
     5341 cgcaccgaaa gggcaggcgc tgccgttcct cttcacgaac acgtacttct ggatcttcaa
     5401 cctgttcgtc gtgccgatca tcacgatgcg gctcttcgcc gaagagcgga agacgggcac
     5461 cttcgagacc ctgctcactt ctcccgtctc ggagacgacc gtcgttctcg gaaaattcac
     5521 cggcgccctc gggttcttcc tcacgctctg ggcgccgacg ctcatttacg tctggattct
     5581 ccggtctcag acgccgatcg acctcgggcc ggtcgccgcc gggtatctcg cgatcacaat
     5641 gatcggcagc tactttctcg ccatcggcat cttcgcctcg acattgacca agaaccagat
     5701 cgtcgcagcg atcctcgctt tcgccatgtt gatcccggtc ttctcggcgg gactcctgga
     5761 gacgctctcg aacgatccga aaaagaccga aacgctcggc tacctgaacc tgtgggacca
     5821 catggaagag ttttcacgag gggtcgtgga cacgcgccgg atcgtttact acctctcggc
     5881 gaccgtgttc ttcctttacc tgacggtcgt ctcgctctcg gcgaagaagg agaacccgtg
     5941 aaccggggtc gcgccgcgcg cgcgggaacg tccgcggccg cgatcgcgct cggcgtcgcc
     6001 cttctcctgg gcgtcaactg gctggggtcg cgtcactggg cgcgaggcga ctggacgaaa
     6061 actcgcatct acagtctctc ctcgaccacc cggaagctca tcgggggtct ctcgaagccg
     6121 gtgcggatca cggtcttcat ggaccgcgat gcgcggctct ggacgccggt ttccgagctc
     6181 ctcgcgcggt atcgagcgct ttccccgaag atcgaggtcg agtatctcga cccgaagcgc
     6241 aacccggcgc gcgccgaggc gctcgtgcgg gagttcggga tccggcagtc gacggtggtc
     6301 ttccgctcgg gcgaccggaa gaagtacgtc gaggaggaca agctcgccga cttcgacttc
     6361 gcgaacgcgc agatgggccg ctccgcccca gagatcaagg ccttcaaagg ggaggaagcg
     6421 tttacatcgg cgatcctggc ggtgaccgag gcgagg
//
LOCUS       DASWAV010000362         2453 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U2665
            k141_299063, whole genome shotgun sequence.
ACCESSION   DASWAV010000362 DASWAV010000000
VERSION     DASWAV010000362.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960316
            Sequence Read Archive: SRR10260007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2453)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2453)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:47:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,929
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2453
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_299063"
                     /isolate="SMAG_U2665"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.36444 N 132.31889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..738
                     /locus_tag="VF958_07560"
     CDS             <1..738
                     /locus_tag="VF958_07560"
                     /inference="COORDINATES: protein motif:HMM:TIGR02777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase ligase N-terminal
                     domain-containing protein"
                     /protein_id="HEX9149001.1"
                     /translation="RRGTALAPDARMGLTEYRKKRRFDVTAEPAGRKAVRPKKRKLSY
                     VIQKHRATALHYDFRLEWKGVLLSWAVPKGPSLDPSVKRMAMPTEDHPIEYAKFEGII
                     PEGEYGGGTVMIWDQGTWTPEVPDVDAALKKGDLKFTLSGKKLHGSWVLVRIRAREGG
                     RPPGLLIKHRDEFATKDDVAEKEPRSVVSRRILAEIARDEGGDIVKAATGDPPALIKK
                     LLKNPELLAPPKRGGRKSVWKSNRAGS"
     gene            749..1699
                     /gene="ligD"
                     /locus_tag="VF958_07565"
     CDS             749..1699
                     /gene="ligD"
                     /locus_tag="VF958_07565"
                     /inference="COORDINATES: protein motif:HMM:TIGR02779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003910 - DNA ligase (ATP) activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="non-homologous end-joining DNA ligase"
                     /protein_id="HEX9149002.1"
                     /translation="MTARGRTARAVPERVPFRVRPMLATLVPEPFHHPGWVYEEKYDG
                     YRILAYKEGDRVTLYSRNAKDRTATFSEIARALERLPDSSLLLDGEAVAFDRKLVSRF
                     QLLQRGDVPYVYAVFDCLYRNGRDLRNEPLPVRRDAVEEAIGDTNHLIPARRLAANGR
                     KAYEQAKRRGYEGVVGKDSAAPYIEGRSTRWLKAKVHQEEEFVIGGYTPPAGSRTHFG
                     ALLLGAYEGSNLNYVGKVGTGFTQKTLAMLHRKLQALLRKDAPFADPPREKGAVWLAP
                     RLVAQIRFQEWTHDWRLRQPVFLGLRDDKSPREVLFPEPG"
     gene            1696..>2453
                     /locus_tag="VF958_07570"
     CDS             1696..>2453
                     /locus_tag="VF958_07570"
                     /inference="COORDINATES: protein motif:HMM:NF045432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9149003.1"
                     /translation="MTASRRGRLPGDSTAPEKLPVRVSNPDKVFWPDEGYTKLDLVRF
                     YAFIFPSLRPWVRDRLLSLKRCPNGLLGKCFFQKEKPDSMPPDTPTRRIVHENGVRNY
                     VVGGKKETQLALANLGCIAVHVWGARAPTPRQPDWVCFDLDPDSGKFADAALAGLKVK
                     TALDALDLVSFAKTSGKKGLHVFVPIRVGPDADEVRWFADALGTKLAAAYPKELTMEV
                     SIAKRKGRVYLDPFRNGFAQTVVSPFCVRRAPRAP"
BASE COUNT          442 a          819 c          831 g          361 t
ORIGIN      
        1 cgacggggca ccgcccttgc accagacgca cgcatgggac tcaccgagta ccgcaagaag
       61 cgccggttcg acgtcacggc ggagccggcc gggcgcaagg ccgtccgacc aaagaagagg
      121 aagctctcct acgtgatcca gaagcatcgc gcgaccgcgc tccactacga cttccgcctc
      181 gagtggaagg gcgtattgct ctcgtgggcc gtgcccaaag gcccttcgct cgatccgtcg
      241 gtcaagcgga tggcgatgcc gaccgaagat cacccgatcg agtacgcgaa gttcgagggc
      301 atcatcccgg aaggggaata cggcggcggc accgtgatga tctgggacca ggggacgtgg
      361 accccggaag tccccgacgt tgacgccgcg ctgaagaaag gggacctcaa gttcacgctg
      421 tccggcaaga agctgcacgg ctcctgggtg ctcgtgcgca tccgggcgcg cgaggggggc
      481 cgcccgcccg ggctcctcat caagcatcgg gacgagttcg cgacgaaaga cgacgtcgcc
      541 gaaaaggaac cgcgctcggt cgtttcccgg cggatcctcg ccgagatcgc gcgcgacgag
      601 gggggcgaca tcgtcaaggc cgcgaccggc gatccgccgg cgctgatcaa gaaactgctc
      661 aagaacccgg agctcctcgc cccgccgaag cgcggcgggc gaaagtcggt ctggaagtcc
      721 aaccgggcgg gctcgtgagg gcggaaccgt gacggcgcgc ggccgcacgg cccgcgcggt
      781 cccggagcgc gtccccttcc gcgtgcggcc gatgctcgcg acgctcgtgc ccgagccttt
      841 tcaccatccg ggctgggtct acgaggagaa gtacgacggc taccggatcc tcgcctacaa
      901 ggagggggac cgggtcacgc tctattcgcg caacgcgaag gaccggacgg cgaccttctc
      961 cgagatcgca cgggcactcg agcggctccc ggactcgagt ctgctgctcg acggcgaggc
     1021 ggtggccttc gaccggaagc tcgtctcccg gttccagctt ctccagcgtg gagacgtccc
     1081 gtacgtctac gccgtcttcg actgcctcta ccgcaacgga cgcgacctcc gcaacgaacc
     1141 gctgcccgtc cgacgcgatg ccgtcgaaga ggcgatcggc gacacgaacc acctgatccc
     1201 ggcgcgccgc ctcgctgcca acggccggaa agcatacgag caggcgaaaa ggcgtggcta
     1261 cgaaggcgtc gtcggcaagg actccgccgc tccgtacatc gaggggcgaa gcacgaggtg
     1321 gctcaaggcg aaagtccacc aggaggagga gttcgtcatc ggcggctata caccgccggc
     1381 cggatcccgc acgcacttcg gagcgctcct gctcggcgcc tacgaggggt cgaacctcaa
     1441 ctacgtcggc aaggtcggca cggggttcac gcagaagaca ctggcgatgc tccaccggaa
     1501 gctccaggcg ctcctccgaa aggacgcgcc cttcgccgat ccaccgcgcg agaagggagc
     1561 ggtctggctg gcgccccggc tggtcgcgca aatccgtttc caggagtgga cacacgactg
     1621 gagactgcgc cagcccgtat ttctcgggct gcgggacgac aagagtcccc gcgaggtcct
     1681 gtttccggag ccggggtgac ggcgtcccgc cgagggcggc ttccgggaga ttcgaccgcg
     1741 ccggaaaagt tgccggtccg cgtgtccaac ccggacaagg tcttctggcc ggacgagggc
     1801 tacacgaagc tcgacctcgt gcgcttctac gccttcatct ttccgagtct ccggccgtgg
     1861 gtgagggatc ggctcctgtc gttgaagcgg tgcccgaacg gcctcctcgg aaagtgtttc
     1921 ttccagaagg aaaagccgga ttcgatgccg ccggatacgc cgaccaggcg aatcgtccac
     1981 gagaacgggg tccggaacta tgtcgtcggg ggaaagaagg agacgcagct cgcgctcgcg
     2041 aacctcgggt gcatcgccgt gcacgtctgg ggcgcacggg cgccaacgcc gcgccagccg
     2101 gactgggtgt gcttcgacct ggatccggat tcgggcaagt tcgccgacgc ggcgcttgcg
     2161 ggcctgaaag tgaagaccgc tctcgacgcg ctcgacctcg tctccttcgc caagacctcc
     2221 ggaaagaagg gcctgcatgt gttcgtgccg atacgggtcg ggccggatgc ggacgaggtt
     2281 cgctggttcg ccgatgcgct cggcacgaaa ctcgcggccg cgtatccgaa agagctgacg
     2341 atggaggtct cgatcgcgaa gcgcaagggg cgcgtctacc tcgatccctt ccgcaacggc
     2401 ttcgcacaga cggtcgtcag tccgttctgc gtgagacgcg ctccgcgcgc gcc
//
LOCUS       DASWAV010000363         4696 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U2665
            k141_299095, whole genome shotgun sequence.
ACCESSION   DASWAV010000363 DASWAV010000000
VERSION     DASWAV010000363.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960316
            Sequence Read Archive: SRR10260007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 4696)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4696)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:47:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,929
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4696
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_299095"
                     /isolate="SMAG_U2665"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.36444 N 132.31889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..716
                     /locus_tag="VF958_07575"
     CDS             <1..716
                     /locus_tag="VF958_07575"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX9149004.1"
                     /translation="VRIIGVVMRSVLLVDKGSLFRDVARSVVRRTNCRILIARTGAEA
                     LAVARREKPDLVFLDAEMEGMTGVDVCRVLKADKQFSHRPIIVVGGGEKERTEGVRAG
                     ADGFLTGNFAEEEFFETLRRHLNVFARDAARSAAGWSITFWRDGAQHQGTIRDLSRGG
                     FFVRTGVEQPIGARLEISFETPVESANRTIVGEAIVVRLGREPDQGLGCRFFQLSSTS
                     RTNLEDCLKMLAPGELAVP"
     gene            complement(722..1165)
                     /locus_tag="VF958_07580"
     CDS             complement(722..1165)
                     /locus_tag="VF958_07580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011554052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dCMP deaminase family protein"
                     /protein_id="HEX9149005.1"
                     /translation="MAERVDWHTYFMNIARQVSTRSTCPRKKVGAVIARDRTILSTGY
                     NGSIRGLPHCEDVGCVIEEGHCVSTVHAEANAIIHAARNGVAIAGAELYTTASPCWPC
                     FKLIANSGIRRVYFGEFYRDEKSLAVARDAGIELVDLEKSASPSA"
     gene            1205..2419
                     /locus_tag="VF958_07585"
     CDS             1205..2419
                     /locus_tag="VF958_07585"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011934549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-[acyl-carrier-protein] synthase
                     family protein"
                     /protein_id="HEX9149006.1"
                     /translation="MTSSRRVVVTGMGAVTSIGASVPDFWKSLLEGVCGIRPLSLFDA
                     SSYRTQTAAEVREIPDGFLSPAERRRMSRADRMGIVAAQEALAQSGLDPAREDPTRIG
                     VILGGGTSGLLDSEEVYEKWLRGRKGRPSRVLNHLPDAITDRVAQRFGLEGIKSTITT
                     ACSSAANAMGYASDVIAAGLADAVVTGGSDVLARLTYGGFNSLRSVDPDPCRPFDRER
                     RGLSIGEAAGILVFEEEEHARRRGATILADFLGYGVTSDAFHMTAPDPSGTAGGRTIR
                     AALEEARVDAADVDYVNAHGTATPQNDSAETAALKSALGDRAKEIPVSSIKSMIGHCL
                     CASGAIEAIATVMTVREGRIPPTIHYENPDPACDLDYVPNEAREHPVSIALSNSFAFG
                     GNSSVVVIGRHG"
     gene            2420..3547
                     /locus_tag="VF958_07590"
     CDS             2420..3547
                     /locus_tag="VF958_07590"
                     /inference="COORDINATES: protein motif:HMM:NF012337.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl synthase N-terminal-like
                     domain-containing protein"
                     /protein_id="HEX9149007.1"
                     /translation="MSTPVVTGAGAVCALGRTSQDFYRRLRAGESAIRPFTPEERGSL
                     QATHVARYDDFKPQPEISPMKARRFDRGSQFAILACSQAVAEAGYDIARSPESVGIAL
                     GTGSAGAGALTEFLRVLLLESPEAAPPFHFPNTVANAPASQVSIELKLFGPNVTITQK
                     DPSGLNALLFASLAISAGRARAMLAGAVDEWNAVYAMGFDRVGALSGEKRASGIIQGE
                     GGFAVLLENEDDARARGARPLARLAGIGTAGVPTEPYRFAPDRRAVARAMRNALSAAG
                     ADARDVHLWLPSRNGVVEMDRAEQAVACEIFGATLPPAIEVKKAIGEMAAAGGAQIVA
                     ACRALSDPDGPFPPDARPRRVLVNSFGAGGNFFSAVFESVP"
     gene            3544..4002
                     /locus_tag="VF958_07595"
     CDS             3544..4002
                     /locus_tag="VF958_07595"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF024675.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HEX9149008.1"
                     /translation="MRSVRDRPPLQGFTIEIPVRFAECDSYGVVWHGHYALYLEEVRD
                     RLTGRFGFTPAKALALGYRVPVTRMEIRYRAPARPDQIVRATARLRPPDTARLVVDYE
                     LRGEEGVLLASAETEQVVLRADGELLLALPAALKRLVGEIIAFQDSGASL"
     gene            4011..4574
                     /gene="xrtH"
                     /locus_tag="VF958_07600"
     CDS             4011..4574
                     /gene="xrtH"
                     /locus_tag="VF958_07600"
                     /EC_number="3.4.22.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR04177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004197 - cysteine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0043687 - post-translational protein
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exosortase H"
                     /protein_id="HEX9149009.1"
                     /translation="MSDNPGSRDPVSARRRSVGFLVRFVVLLVVFYLIVASRPVNDAV
                     IVPFTAGIASVSGKLLNVLGERAEVVGTEIRSSSFGVNIENGCNGVETALLFGAAVLA
                     FPASWKRRLLGLIAGFVAIQVLNLFRVISLFWIGLHRPALFSSSHTVIWQSVVVLFGV
                     LLFLVWASRERRLSAESSSAGERPGRA"
BASE COUNT          681 a         1599 c         1599 g          817 t
ORIGIN      
        1 gcgtccgtat aatcggggtc gtcatgcgtt ccgtcctcct cgtggacaaa gggagccttt
       61 tccgcgacgt cgcacgctcg gtcgtccggc ggacgaactg ccgcatcctg atcgccagga
      121 cgggcgcgga ggcgctcgcg gtcgcgcgcc gcgagaagcc ggacctcgtc tttctcgacg
      181 cggagatgga agggatgacg ggagtggacg tctgccgcgt gctgaaggcg gacaagcagt
      241 tctcgcaccg cccgatcatc gtcgtcggcg gcggggagaa ggagcgcacc gagggggtgc
      301 gggccggcgc ggacggtttc ctgacgggaa atttcgcgga ggaggagttc ttcgagacgc
      361 tgcgccgtca cctgaacgtc tttgcgcgcg acgcagcgcg ttcggccgcc ggatggtcaa
      421 ttacgttctg gcgggatgga gcccagcacc aggggacgat ccgagacctc tcgcgcggcg
      481 gttttttcgt gagaaccggg gtggagcaac cgatcggcgc gcgcctggag atctccttcg
      541 agacgccggt ggagagcgca aaccgcacca tcgtcggcga ggccatcgtc gtccggctcg
      601 ggcgcgagcc ggatcaggga ctcggctgcc gtttcttcca gctctcctcg acctcgcgta
      661 cgaacctgga ggattgcctg aagatgctcg ccccgggtga gctcgccgtg ccctgacgtc
      721 gctacgctga aggcgaagcg ctcttttcga gatccaccag ctcgatgccc gcgtcgcgcg
      781 ccacggcgag cgatttctcg tcgcggtaga actctccgaa gtagacgcgg cggataccgg
      841 aattcgcgat cagcttgaag cacggccagc acgggcttgc cgtggtgtag agctcggcgc
      901 ccgcgatcgc aacgccgttt cgcgccgcgt ggatgatggc gttcgcctcc gcgtggacgg
      961 tcgaaacgca gtgcccctcc tcgatcacgc agcccacgtc ttcgcagtgc ggcagcccgc
     1021 gaatcgagcc gttgtagccg gtcgagagaa tcgtccggtc gcgcgcgatg accgcgccga
     1081 cctttttgcg aggacaggtc gagcgcgtgg agacctgccg cgcgatgttc atgaagtacg
     1141 tgtgccagtc gacgcgttcg gccatcgcgt ccggattatg cctccatgtg tagaatcgcc
     1201 cgccatgact tcgagccggc gcgtcgtagt gacgggtatg ggggccgtca cgtccatcgg
     1261 cgcctcggta ccggatttct ggaaaagcct tctcgaggga gtttgcggaa ttcggcccct
     1321 atcgctgttc gacgcgtcgt cctaccggac gcagacggcc gcggaagtcc gggagattcc
     1381 cgacggcttc ctctcccccg ccgaaagacg gcggatgtcg cgggccgatc ggatgggaat
     1441 cgtcgccgcg caggaggcgc ttgcccagtc gggactcgat ccggcgagag aggacccgac
     1501 gcgtatcggg gtgatcctgg gcggcggcac ctcgggtctc ctcgactccg aggaggtcta
     1561 cgagaagtgg ctgcgtggcc ggaaaggccg gccgtcgcgg gtcttgaacc acctgcccga
     1621 cgcgatcacc gatcgggtcg cgcagcggtt cgggctggag ggaatcaagt cgacgatcac
     1681 gaccgcgtgc tcgtcggcgg cgaacgcgat gggctacgcc agcgacgtga tcgcggcggg
     1741 actcgccgat gccgtcgtga ccggcggttc ggacgtcctc gcgcggctca cctatggcgg
     1801 gttcaactcg ctgcgttccg tcgatccgga tccctgccgc ccattcgacc gcgagcggcg
     1861 gggcctttcc atcggcgagg ccgccggcat cctcgtattc gaggaggagg agcatgctcg
     1921 gcggcgcggc gccacgatcc tcgccgactt cctcggatac ggcgtcacat cggatgcatt
     1981 ccacatgacg gctccggacc cctcggggac cgcgggcggc cgcacgatcc gagccgcgct
     2041 cgaggaggct cgcgtcgacg ccgccgacgt cgattacgtc aatgcccacg ggacggccac
     2101 gccgcagaac gactccgccg agaccgccgc gctgaagtcg gcgctcggag accgcgcgaa
     2161 ggagatcccc gtctcgtcga tcaagtcgat gatcggccac tgcctgtgcg cgtccggcgc
     2221 gatcgaggcg atcgccacgg tcatgaccgt acgggagggg cgcattcctc cgacgatcca
     2281 ctacgagaat cccgatcccg cgtgcgacct cgactacgtt cccaacgagg cgcgcgagca
     2341 tccggtctcg atcgccctct cgaactcctt cgcgttcgga ggaaacagct cggtggttgt
     2401 catcgggcgc cacgggtaat tgagcactcc cgtcgtcacc ggcgcgggcg ctgtgtgcgc
     2461 cctcggtcgg acctctcagg atttctaccg gcgacttcgc gccggcgaga gcgcgatccg
     2521 gccgttcacg ccggaggagc gcggctcgct ccaggcgaca cacgtcgcgc gctacgacga
     2581 tttcaagccg caacctgaga tctccccgat gaaggcgcgc cggttcgacc gcggcagcca
     2641 gttcgcgatc ctggcgtgct cgcaggccgt ggcagaggcc ggctacgaca tcgcccggtc
     2701 gcccgagtcc gtcgggatcg cgctcggaac cggatccgcg ggagccggag cgctcacgga
     2761 gttcttgcga gtgctgctcc tcgagtcccc cgaagccgcg ccgcccttcc actttccgaa
     2821 cacggtggcc aatgcgccgg cgtcgcaggt ttccatcgag ctcaagctct tcggaccgaa
     2881 cgtgaccatc acacagaagg atccgtcggg tctgaacgcg ctgcttttcg cctccctcgc
     2941 gatctccgcc gggcgggcgc gagccatgct cgcgggcgcc gtcgacgaat ggaacgccgt
     3001 ctacgcgatg ggcttcgatc gcgtcggggc gctctccggg gaaaagcggg cctccggaat
     3061 tatccagggc gaaggcgggt tcgccgtcct cctcgagaac gaggacgacg cgcgcgcgcg
     3121 cggcgcccgg cctctcgcgc gcctcgccgg catcgggacg gccggcgtgc cgaccgagcc
     3181 ttaccggttc gcgcccgatc gcagggcggt ggcccgtgcg atgcggaacg ccctttcggc
     3241 agcgggtgca gatgcccggg atgtccacct ctggctgcct tcgcgcaacg gagtcgtgga
     3301 gatggatcgc gcggagcagg cggtcgcgtg cgagatcttc ggagcgacgc tgccgccggc
     3361 gatcgaggtg aagaaagcca ttggcgaaat ggcggcggcc gggggggcgc agatcgtcgc
     3421 ggcgtgccgt gctctttccg atcccgacgg accttttccc ccggacgcgc ggccgcgtcg
     3481 ggtgctcgtc aactccttcg gcgccggagg caactttttc tccgcagtct tcgagtccgt
     3541 gccatgagat cggtccggga tcggccgccg cttcagggct tcacgatcga gatcccggtg
     3601 cgcttcgccg agtgcgactc gtacggcgtc gtctggcacg gccactacgc gctctacctc
     3661 gaagaggtgc gcgaccggct gaccggccgg ttcggcttca cgccggcgaa agctctcgcg
     3721 ctcggatacc gggttcccgt gacccgcatg gagattcgct accgcgcgcc cgcccggccg
     3781 gaccagatcg tccgcgcgac cgcgagactg cgtccacccg atacggcgcg gctcgtcgtc
     3841 gactacgagc tgcgcggaga ggaaggcgtg ctcctcgcat ccgcggaaac ggaacaggtc
     3901 gtgttgcgcg ccgacggcga gcttctgctg gcgcttcccg cggcgttgaa gcggctcgtc
     3961 ggagagatca tcgcgttcca ggattcgggc gcctcgctat aatcccgcgg atgtcggaca
     4021 accccggctc gcgagacccc gtttcggcgc gccggcgctc cgtcggcttc ctcgttcggt
     4081 tcgtggtcct tctcgtcgtg ttctacctga tcgtcgcgtc ccgcccggtc aacgacgccg
     4141 tcatcgtgcc gttcaccgcc ggaatcgcgt cggtctccgg aaagctcctg aacgtgctcg
     4201 gcgagcgggc ggaggtcgtc ggcaccgaga tccgctcctc ctctttcgga gtgaacatcg
     4261 agaacggctg caacggcgtc gaaaccgccc ttctgttcgg cgcggcggtc ctcgcctttc
     4321 cggcgagctg gaagaggcgg ctgctcggac tcatcgccgg gttcgtcgcg atccaggtgc
     4381 tgaacctgtt ccgggtcatc tcgctgttct ggatcggcct ccaccgcccc gcgctgttca
     4441 gctcgtctca caccgtcatc tggcagtccg tggtggtcct cttcggcgtg ctcctcttcc
     4501 tggtctgggc gtcgcgcgaa agacgacttt ccgccgaatc ctcgtcggcg ggagaacgtc
     4561 ccgggagggc ttgaacggcc gcgcgagcgc agtcaccggg cgtgcggtct ccagtgctca
     4621 cgcctcgcgc cctgctgcgc gcgctcgtgg ggttcctcct cgcgatcgcc ttctggttcg
     4681 gcttctcgcg ccccta
//
LOCUS       DASWAV010000364         2559 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U2665
            k141_299113, whole genome shotgun sequence.
ACCESSION   DASWAV010000364 DASWAV010000000
VERSION     DASWAV010000364.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960316
            Sequence Read Archive: SRR10260007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2559)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2559)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:47:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,929
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2559
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_299113"
                     /isolate="SMAG_U2665"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.36444 N 132.31889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..329)
                     /locus_tag="VF958_07605"
     CDS             complement(<1..329)
                     /locus_tag="VF958_07605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid decarboxylase"
                     /protein_id="HEX9149010.1"
                     /translation="MSQSSFDLPPHEVRRLGYLAADAVAEHRKRLTSRPVFGKVGRDA
                     SLFREPLPQEGLPFEEVLAFVREHVMPFPFGNSHPRFFGFINATADPVGITADYLAAA
                     MNPNCWGG"
     gene            complement(411..965)
                     /locus_tag="VF958_07610"
     CDS             complement(411..965)
                     /locus_tag="VF958_07610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012241040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006801 - superoxide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase family protein"
                     /protein_id="HEX9149011.1"
                     /translation="MKTSRISTLAAGLAAAAGTLLVTACASTPKAAAGPQARATIEAR
                     SGSNVTGWATFTERSTGGVAVVVHVENAPPGTHGLHVHEKGDCSAPDASSAGGHFNPG
                     GMPHAGPTDMHRHAGDLGNITIESNGTGHLELVTDLLTVRPGPNSVVGKAVVFHEKTD
                     DLSTQPTGNAGGRLGCGVVALASP"
     gene            1087..>2559
                     /locus_tag="VF958_07615"
     CDS             1087..>2559
                     /locus_tag="VF958_07615"
                     /inference="COORDINATES: protein motif:HMM:NF017044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HEX9149012.1"
                     /translation="MRKLLRLIVAVLALAASAAPLSAQKISVVAKRLSNGMDLLVVRR
                     EGEATVAGGWVAHVGSSNERPGITGISHLFEHMMFKGTPTIGTKDYKQDVAIIAEQEK
                     VRDEIRQEEAKMRAAYRRGEIDDVLKPENKTPRLRELEKTFDELIAKQRKVLVKNEFD
                     RIYTTAGGSGMNAFTNEDMTGYFITVPSNKVELWMWMESERLLHPVFREFYAERDVVF
                     EERRLRTEATPLGKYEETFESMFWESHPYNWPVVGWPSDIPAITKAQADAYYATYYAP
                     QNIALILVGDLDAEKVAAGAERYFGRIPRGKSAVPDVVTLEVPQQAEKRLNAEADTNP
                     QVDILWHTVPFGHADSYALQVLAQLLSTRTGRLYKGLVLGSQVATEAFARQESRKWAG
                     LFNAGGEAKEGHRPEEVEQAIYGEIDKLKKDDVPAEELQKVKNNFAASEYRRMSSNFP
                     ILLQLIQSDGRGDWHEVNEAGTKVQAVTAADVKRVANKYFT"
BASE COUNT          468 a          810 c          891 g          390 t
ORIGIN      
        1 ccgccccagc agttcggatt catcgcggcc gcgagatagt cggccgtgat cccgacggga
       61 tcggcggtcg cgttgatgaa cccgaagaat cgcgggtgcg agttgccgaa cgggaacggc
      121 atcacgtgct cgcgaacgaa cgcgaggacc tcctcgaatg gcaggccctc ctgcggcagc
      181 ggctcgcgga agagggacgc gtcacgcccg accttgccga acaccggccg tgaggtgagt
      241 ctcttgcggt gttccgcgac cgcgtcggcc gcgaggtagc cgagtcgccg gacttcgtgg
      301 gggggaagat cgaatgaaga ctgactcatg cacgcattga atcatcgtcg ggacagatga
      361 aacgggccgg cgcggccgcc ggcccatgtc ccgcctgcgg ctcgagacga ctacggagaa
      421 gccagagcga ccactccgca accgagccgg cccccggcat tgcccgtggg ctgagtcgac
      481 aggtcgtcgg tcttctcgtg aaaaacgacg gccttcccga cgacggaatt cgggcccggc
      541 cgcacggtca gcaggtccgt cacgagctcc aggtgccccg tcccgttcga ctcgatcgtg
      601 atgttgccga ggtctcccgc gtgccggtgc atgtcggttg gcccggcgtg cggcatcccg
      661 cccggattga agtgtccgcc tgccgacgag gcgtcgggcg cggagcagtc ccccttctcg
      721 tggacgtgaa gtccgtgcgt cccgggcggg gcgttctcga cgtggacgac gaccgcgacg
      781 ccgccggtcg accgctcggt gaacgtcgcc cagccggtca cgttggaccc gctgcgggcc
      841 tcgatcgtag cgcgcgcctg cggtccggcc gccgccttcg gggtggaagc gcatgcggta
      901 acgagtaagg tcccggccgc cgccgcgagc ccggccgcga gagtggagat tcgcgatgtc
      961 ttcatttttc ctccttcgcg ttcccgatga tagaagatga gctggtacat ccctgcaagg
     1021 ggcgttccgg ggaattcggg acgtgttccg agcgcggcgc gaaggtgccg cgacgaaagg
     1081 ccaggaatga ggaaattgct ccggctgatc gtggccgtcc tggcgctcgc ggcttccgcg
     1141 gcgccgctct ccgcgcagaa gatcagcgtc gtcgcaaagc gactgtccaa cggcatggac
     1201 ctgctggtgg tccgccgcga gggggaggcg accgtcgccg ggggctgggt cgcacacgtc
     1261 gggagttcca acgagaggcc cgggatcacg gggatctcgc acctcttcga acacatgatg
     1321 ttcaagggca cgcccacgat cgggacgaag gactacaagc aggacgtcgc catcatcgcc
     1381 gagcaggaga aggttcgaga cgagatccgg caggaggagg cgaagatgcg cgccgcctac
     1441 cgtcggggcg agatcgacga cgtgttgaag ccggagaaca agacgccccg gcttcgggag
     1501 ctcgagaaga ccttcgatga gctcattgcg aagcagagaa aggttctggt caagaacgag
     1561 ttcgaccgga tctacaccac cgcgggcggc tcggggatga acgccttcac caacgaggac
     1621 atgaccgggt acttcatcac cgtaccgtcg aacaaggtcg agctctggat gtggatggag
     1681 tcggagcgtc tcctccaccc ggtcttccgc gagttctacg cggagcgcga cgtcgtcttc
     1741 gaggagcgac gtcttcgcac ggaggcgacg ccgctcggca agtacgagga gacgttcgag
     1801 tccatgttct gggagtcgca cccctacaac tggccggtcg tgggctggcc ctcggacatc
     1861 ccggcgatca cgaaggcgca ggcggacgcc tattacgcga cctactacgc cccgcagaac
     1921 atcgcgctca tactcgtcgg cgacctcgac gccgagaagg tcgcggccgg ggcggagcgc
     1981 tacttcggcc gcatcccgcg tggaaagagc gccgtgcccg acgtcgtgac actcgaggtc
     2041 ccgcagcagg cggagaagcg cctgaacgcg gaggcggaca cgaacccgca ggtcgacatc
     2101 ctctggcata cggtcccctt cggccacgcg gactcgtatg cgctccaggt cctggcacag
     2161 ctcctctcga cccgcacggg gcggctgtac aaggggctcg ttctcggatc gcaggtcgcg
     2221 acggaggcgt tcgcgcgtca ggagtcgcgc aagtgggcgg gtctcttcaa cgccgggggc
     2281 gaggcgaagg aaggccatcg tcccgaggag gtcgagcagg cgatctacgg cgagatcgac
     2341 aaactgaaga aggacgacgt tccggccgaa gagctccaga aggtgaagaa caacttcgcg
     2401 gcgtcggaat accggcgcat gtcgtcgaac tttccgatcc tcctccagct cattcagagc
     2461 gacggccgcg gggactggca cgaggtgaac gaggcgggca cgaaggtgca ggccgtgacg
     2521 gccgccgacg tgaagcgggt cgcgaacaag tacttcacg
//
LOCUS       DASWAV010000365         3473 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U2665
            k141_338131, whole genome shotgun sequence.
ACCESSION   DASWAV010000365 DASWAV010000000
VERSION     DASWAV010000365.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960316
            Sequence Read Archive: SRR10260007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 3473)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3473)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:47:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,929
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3473
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_338131"
                     /isolate="SMAG_U2665"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.36444 N 132.31889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..749)
                     /locus_tag="VF958_07620"
     CDS             complement(<1..749)
                     /locus_tag="VF958_07620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010937745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HEX9149013.1"
                     /translation="MNISDIFIRRPVMTTLIMIGILLFGVMGYRLLPVSDLPNVDFPT
                     IQVSASLPGASPETMASAVATPLEKQFTTIAGLDSMNSTSIQGNTQITLQFNLSRNLD
                     SAAQDVQSAIAVANRQLPRDMPSPPSYQKVNPADSPILYIALTSPTLPLYELDEYGQT
                     MMAQRISTISGVAQVSVYGSQKFAVRVQLDPRQLVTRGIGIDEVSGAVQTANVNLPTG
                     TLYGANKAFTVQANGQLTDAEGYRSIVVAYR"
     gene            complement(746..1879)
                     /locus_tag="VF958_07625"
     CDS             complement(746..1879)
                     /locus_tag="VF958_07625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011939244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HEX9149014.1"
                     /translation="MRIERSGARIAFAILAAATVAWTGCAKKDPRAGAAAEAAPVVVA
                     NVARQDVPVEVRAVGHVEAYTTVSLTARVGGQVTRVGFREGEDVRRGDLLFQIDPRPY
                     QAALAQARAQLERDRAIARNAEQDVKRYTDLVQKDYVTREQYDATRANAAAALASAKA
                     DEAAVENAKLQLSYCTVTAPISGRTGRVLVNPGNMVKGNDENPLVVLNQIQPVYVSFS
                     VPESSLARIREEIRPAGKLKVAAFPSGDPSRTQTGELSFVNNTVDPGTGTILLKGTFP
                     NENEMLWPGEYVDVVLTLATEPRAIVVPSQAVQTGQSGQYVWVVKSDLTVESRPVTVA
                     RTQGPLTVVAKGLEAGERVVTDGQLRLAPGARVEIRKVEGKTA"
     gene            complement(1876..3354)
                     /locus_tag="VF958_07630"
     CDS             complement(1876..3354)
                     /locus_tag="VF958_07630"
                     /inference="COORDINATES: protein motif:HMM:NF014385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HEX9149015.1"
                     /translation="MIGRRRRAFAVLAPVATVLSLAGCASGDVDARVSPNPATLWQPP
                     PEGRMPAPPPTPPPAIPPEYVKPGTTVSLEQIIDVALRNNPVTRTAWFNARAAAANVG
                     AKRAEYFPTLELDANLTRQKQTAVGGRFVTLQTTYGPSLNLSWLLFDLGGRAADVDEA
                     RNALFAADWTHDAVIQNLVLQVEVAFYQYQNAKAQAAAAEATLTQARESLKAAEDRHQ
                     AGVATIADVLQAKTAVSQAELVLETAQGSIQTLRGALATAAGVSPDVPVDVAPLPDQV
                     NVEEVGETVQALIEKAEAQRPDLAAARFEALKAQSHVRSVRSEGLPTLAAAGSASRVY
                     FYNSSGLPFANNYSGSILFRFPVFTGGRNTYETLEAKEQARAAEGQAETLQDQVVLQV
                     WTSYYNLKTAAQRVKTARDLLASASQSQDVAAGRYRAGVGSILDLLVAQSAYANARAQ
                     EAQARSDWFVALALLAHDTGSLGPPSGPVQPVSAGETKGAVP"
BASE COUNT          533 a         1159 c         1180 g          601 t
ORIGIN      
        1 tcgcgatacg ccaccacgat ggaccgatac ccttccgcgt ccgtcagctg tccgttcgcc
       61 tgaaccgtga acgccttgtt cgccccgtaa agcgtgccgg tcggcaggtt cacgttcgcg
      121 gtctggacgg cgccggagac ctcgtcgatt ccgatgcccc gcgtgacgag ctgccgcgga
      181 tcgagctgga cccgcaccgc gaacttctgt gagccgtaga cggagacctg cgcgactccc
      241 gagatggtcg aaatccgctg cgccatcatg gtctggccgt actcgtcgag ctcgtagagg
      301 ggaagcgtcg gcgaagtcag cgcgatgtag aggatcggcg agtctgcggg gttgaccttc
      361 tggtacgagg gggggctcgg catgtcgcgc ggcagctgcc ggtttgccac ggcgatcgcg
      421 gactgcacgt cctgggccgc cgaatcgagg ttccgcgaca ggttgaactg gagcgtgatc
      481 tgcgtgttcc cctggatgct cgtcgagttc atggaatcga ggccggcgat cgtggtgaac
      541 tgtttttcga gcggggtcgc gaccgccgaa gccatggtct ccggcgaagc gccgggcaaa
      601 ctcgcggaga cctggatggt ggggaagtcc acgttgggca gatccgagac cggcaggagc
      661 cggtacccca tgaccccgaa gaggaggatg ccgatcatga tcagcgtggt catgacggga
      721 cggcggatga agatgtcgga aatgttcatg cagtcttccc ttcgaccttc cggatttcca
      781 cgcgcgcccc gggtgcgagc cgcagctgcc cgtcggtgac gaccctctcc ccggcctcca
      841 atcctttcgc gaccacggtg agcgggccct gggtgcgagc caccgtcacc ggtcgcgatt
      901 ccaccgtcag gtcgcttttc acgacccaga cgtactggcc cgactggccc gtctgcaccg
      961 cctggctcgg aacgacgatc gccctcggct cggtcgcgag cgtcagcacg acgtcaacgt
     1021 actcccccgg ccagagcatt tcgttctcgt ttggaaaagt ccccttgaga agaatcgtac
     1081 ccgtgcccgg atccacggtg ttgttgacga agctcaactc acccgtctgc gtccgcgacg
     1141 gatcgcccga ggggaatgcc gcgaccttca gcttcccggc cggacggatc tcctcgcgga
     1201 tccgggcgag agaagactcg ggcaccgaga aagaaacgta aaccggctga atctggttga
     1261 ggaccacgag agggttctcg tcgttgcctt tcaccatgtt gccgggattc accaggaccc
     1321 ttccggtgcg gcccgagatg ggagccgtca ccgtgcagta ggacagctgc agcttcgcgt
     1381 tctcgacggc ggcctcgtcg gctttcgccg aagcgagcgc ggccgcggcg ttggcgcgtg
     1441 tcgcgtcgta ctgttcgcgg gtcacgtagt ccttctggac caggtcggtg taccgcttga
     1501 cgtcctgttc ggcattgcgg gcgatcgccc ggtcgcgctc gagctgggct cgggcctggg
     1561 caagagccgc ctggtatgga cgcgggtcga tctggaagag aagatcgccc cgacgcacgt
     1621 cctcgccctc ccggaatccg actctcgtca cctgcccgcc cacgcgcgcc gtgagcgaga
     1681 cggtcgtgta ggcctcgacg tgtccaaccg cgcggacctc caccgggacg tcctgccgcg
     1741 cgacattcgc gacgaccacg ggcgccgcct cggcagccgc gcccgcccgc gggtccttct
     1801 tcgcgcaacc ggtccaggcg accgttgctg ccgcaaggat cgcgaaggcg attcgtgcac
     1861 cacttctctc gattctcacg gcacggctcc ctttgtttct ccggcagata ccggctggac
     1921 gggtccggac ggcgggccga gcgagccggt atcgtgcgcg agcagggcga gcgcgacgaa
     1981 ccagtccgaa cgcgcctgcg cctcctgcgc ccgggcgttc gcgtaggcgc tttgcgcgac
     2041 gaggaggtcg agaatcgatc ccacgcccgc acgatatcgg cccgcggcga catcctggga
     2101 ctgcgatgcc gacgccagca ggtcgcgcgc cgtcttcacg cgctgcgccg cggttttcag
     2161 gttgtaatag ctcgtccaga cctggagcac gacctggtcc tggagtgtct ccgcctggcc
     2221 ttccgccgcc cgcgcctgct ccttcgcttc gagagtctcg taggtgttcc gtccgccggt
     2281 gaaaaccggg aagcggaaga ggatcgaacc cgagtaattg ttcgcgaacg gaaggccgct
     2341 cgagttgtag aagtagacgc ggctcgccga gcccgcggcg gcgagcgtgg ggagaccttc
     2401 ggagcggacc gaccgcacgt ggctctgcgc cttgagcgcc tcgaaccgcg cggccgcgag
     2461 gtccggacgc tgcgcctccg ctttttcgat caatgcctgg accgtctctc cgacttcttc
     2521 gacgttcacc tggtcgggca gcggcgcgac gtcgaccggg acgtcgggcg acacgccggc
     2581 cgcggtcgcg agtgcgccgc gcagcgtctg gatcgagccc tgcgccgttt cgagcacgag
     2641 ctccgcctga gagaccgccg ttttggcctg gaggacgtcg gcgatcgtgg cgacccctgc
     2701 ctggtgccgg tcttccgcgg ccttcaggct ttcgcgcgct tgggtcagag tcgcctcggc
     2761 cgccgcggcc tgcgccttcg cgttctggta ctgatagaag gcgacctcga cctggagcac
     2821 caggttctga atcaccgcgt cgtgagtcca gtcggccgcg aagagggcgt tgcgcgcctc
     2881 gtccacgtcc gcggcgcggc ctccgaggtc gaagagcagc caggaaaggt tcagcgacgg
     2941 gccataagtg gtctgcagcg tgacgaaccg accgccgacc gcagtctgct tctggcgagt
     3001 cagattggcg tcgagctcga gcgtcgggaa gtattccgcg cgcttcgcgc cgacgttcgc
     3061 cgcggccgcc cgcgcattga accacgcggt ccgcgtcacc ggattgttgc gaagcgcgac
     3121 gtcgatgatc tgctcgagag agacggtggt gccgggcttg acgtactccg gcgggatcgc
     3181 ggggggcggg gtcggagggg gcgcaggcat ccggccctcc ggcggcggtt gccacagtgt
     3241 ggcgggattc ggtgagacgc gggcgtccac gtcccccgaa gcgcagccgg ccagagagag
     3301 gacggtcgcg accggagcga ggaccgcgaa ggcgcggcgg cgacgtccga tcacccggcg
     3361 tgcctcacga ttccccgcgt gaagatgccg acgatctcgg cggacaattg ctccaacgtt
     3421 tcctccccca cccgaaggcg gttcgcggca cgaatcattc cccggaagat ctc
//
LOCUS       DASWAV010000366         3343 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U2665
            k141_460344, whole genome shotgun sequence.
ACCESSION   DASWAV010000366 DASWAV010000000
VERSION     DASWAV010000366.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960316
            Sequence Read Archive: SRR10260007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 3343)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3343)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:47:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,929
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3343
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_460344"
                     /isolate="SMAG_U2665"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.36444 N 132.31889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..279)
                     /locus_tag="VF958_07635"
     CDS             complement(<1..279)
                     /locus_tag="VF958_07635"
                     /inference="COORDINATES: protein motif:HMM:NF019095.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHRD domain-containing protein"
                     /protein_id="HEX9149016.1"
                     /translation="MRCGGVLLVLLAGAIGCAAAPRPPEYCAELKGAGPGSDALDPDA
                     SGSARLVVGETSIRFHVTTSANLGKVVATHLHNGTAGVNGPMAVELNPG"
     gene            complement(276..1529)
                     /locus_tag="VF958_07640"
     CDS             complement(276..1529)
                     /locus_tag="VF958_07640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012875426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004013 - adenosylhomocysteinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylhomocysteinase"
                     /protein_id="HEX9149017.1"
                     /translation="MSATSSIVADFALAPEGAKKIRWVAQHAPVLNAAFERRLCDGSL
                     RGVNVAVCVPLEAKTAYLTLLLKRAGANVALAGTAPGYVQDDVAAAIAQEGVIVFADA
                     RASAEEFEGFLVGTIRTDPALLVDDRGELTRILHTKETARLPHVWGATEQTTSGVTKL
                     RNMEREGILRYPIIAGNDAMCKHLFDNRYGTGQSTFTAILALTNLYLGGRIVVIAGYG
                     WCGKGLARRAAGLQSRVVVCEVDPVRALEAVADGFRVLPLELAAEIGDFFVTSTGTHG
                     VFRGKHFERMKDGAVLANAGGLDTEIDVPALAAMAVEVREARRPITEYRLADGRRLHL
                     LAEGKLVNIAGGDGHPVEIMDLSFTVQALAIHYLARHHRELTPGVHPLPAEIDREIAA
                     IKLETLGVSIDSLTAEQVEIRRRWQ"
     gene            complement(1526..2773)
                     /locus_tag="VF958_07645"
     CDS             complement(1526..2773)
                     /locus_tag="VF958_07645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009709107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004013 - adenosylhomocysteinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylhomocysteinase"
                     /protein_id="HEX9149018.1"
                     /translation="MPASTIRDISLAPEGERKIQWVAQHAPTLNSFAAERLSDGALRG
                     LRIAIAVHLEAKTAYLAWLFHEAGAQVAATGSNPYSTQDDVCAALVARGVTVHAIHAA
                     DPRTFDEHLLATLDFEPDLIVDDGAELVTRLHQQRRDLLPRVRGASEETTSGVMRLRA
                     MEQEGALKIPVIVANEARCKHMFDNRYGTGQSALTAILSATNLLLAGKVVVVAGYGWV
                     GQGLALYCRGFGARVVVVEVDPIRGLEAAANGFDVSTMADAAPIGDVFVTGTGSIGVL
                     RAEHFGKMKDGALLANAGNYAHEIDVPALAEMAVEEREARRHITEYALGDGRKIHLLA
                     HGALVNIAASDGHPVEIMDMSFSVQMLSIHHLARHAGGMRPGVHALPAEIDDAIARRR
                     LELLGMRLEEPTRDQLEYARSWR"
     gene            complement(2776..>3343)
                     /locus_tag="VF958_07650"
     CDS             complement(2776..>3343)
                     /locus_tag="VF958_07650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9149019.1"
                     /translation="VGRDVLIGCIMGSVIGTVSRLAIVVGPRLGVPPRPQDVLPPLLL
                     GTRKVIAETVSMLPDAIFIGFGMLLVLFLLRMLLRSEWAAATAFVLLLGIGRVLESDV
                     KLLATVTGIIVVGGIALVFIRFGLVAAITTLYFDFLVNQFPFTTDTSSWYFGVGLFAV
                     ALMAAIAIAAFRISLGGRPVFGGARLED"
BASE COUNT          572 a         1155 c         1100 g          516 t
ORIGIN      
        1 tccggggttg agctcgaccg ccatcggacc gttcacgccg gccgtcccgt tgtgaaggtg
       61 cgtcgccacg accttgccga ggttcgcgga cgtcgtgacg tggaagcgga tcgacgtttc
      121 gccgaccacg agccgggccg atccgctcgc atccgggtcg agcgcgtcgg acccgggccc
      181 cgcccctttg agctccgcac agtactccgg cgggcgcggc gccgccgcgc agccgatcgc
      241 cccggcgaga agcacgagga gaacgccgcc gcacctcatt gccagcgtcg ccgaatttcg
      301 acctgctccg cggtcaacga gtcgatcgag acgccgagag tttcgagctt gatcgccgcg
      361 atctcgcggt cgatctcggc cggcagcggg tggactccgg gagtcagctc ccggtggtgc
      421 cgcgcgaggt agtggatcgc gagagcctgc acggtaaagg aaaggtccat gatctcgacc
      481 gggtgcccgt cccctccggc gatgttgacg agctttccct ccgcgaggag gtgcagccgc
      541 cgtccgtcgg caagccggta ttccgtgatc gggcgcctcg cttcgcgcac ctcgaccgcc
      601 atcgccgcga gagccgggac gtcgatctcg gtgtcgagac cgccggcgtt cgcgagaacc
      661 gccccgtctt tcatccgctc gaagtgcttt ccgcgaaaaa cgccgtgcgt cccggtcgac
      721 gtgacgaaga agtcgccgat ttccgccgcc agctccagcg gcaggacgcg gaacccgtcg
      781 gccaccgctt cgagcgcgcg gacggggtcg acctcgcaca cgacgactcg cgactgcagt
      841 cccgccgcgc ggcgcgcgag cccttttccg caccagccgt aaccggcaat cacgacgatc
      901 cggccgccga gatacaggtt ggtcagcgcg aggatcgccg tgaacgtcga ctgtcccgtg
      961 ccataccggt tgtcgaacag gtgtttgcac atcgcgtcgt tgcccgcgat gatcggatag
     1021 cgcaggattc cttcccgctc catgttccgg agcttcgtga cgcccgacgt cgtctgctcg
     1081 gtcgcgcccc acacgtgcgg cagccgcgcg gtctctttcg tgtggaggat gcgcgtcaac
     1141 tcgccgcggt cgtccacgag gagagcgggg tccgtccgga tcgttccgac cagaaatccc
     1201 tcgaactctt cggcggaggc ccgggcgtcg gcgaagacga tcacgccctc ctgcgcgatc
     1261 gcggccgcga cgtcgtcctg aacgtaaccc ggggcggtgc cggcgagcgc cacgtttgca
     1321 ccggcgcgct tcaggaggag cgtcaggtac gccgtcttcg cctccagcgg gacgcaaacg
     1381 gcgacgttga cgccgcggag cgacccgtcg cagaggcgtc tctcgaacgc cgcgttcagc
     1441 accggcgcgt gctgcgcgac ccaccggatc ttcttcgcgc cctccggcgc gagcgcgaaa
     1501 tccgcgacga tcgaagaagt cgcgctcatc gccagctccg tgcgtactcg agctggtcgc
     1561 gcgtcggttc ctcgagacgc atgccgagga gctcgagccg ccgccgcgca atcgcatcat
     1621 cgatctcggc gggaagagcg tggacgcccg gccgcatccc tcccgcgtgc cgcgcgaggt
     1681 ggtgaatcga gagcatctgc accgagaagg acatgtccat gatctccacc gggtggccgt
     1741 cggaagcggc gatgttgacg agcgctccgt gcgcgaggag atggattttc cgcccgtcgc
     1801 cgagcgcgta ctcggtgatg tgcctgcgcg cctcccgctc ctcgacggcc atttcggcga
     1861 gcgccggcac gtcgatctcg tgggcgtagt tgccggcgtt ggcgaggagc gccccgtcct
     1921 tcatcttccc gaaatgctcc gcgcgcaaca cgccgatcga acccgtgccg gtgacgaaga
     1981 cgtcgccgat cggcgccgcg tctgccatcg tggaaacgtc gaatccgttc gcagccgcct
     2041 cgagcccgcg gatcgggtcc acctcgacca cgacgacgcg ggcgccgaag ccgcggcagt
     2101 agagcgcgag cccctgtccg acccagccgt accccgcgac gacgacgacc ttgcctgcca
     2161 gcagcaggtt cgtggccgag agaattgccg tcagagccga ctgtccggtt ccgtagcggt
     2221 tgtcgaacat gtgcttgcac cgcgcctcgt tcgcgacgat caccgggatc ttcaacgccc
     2281 cctcctgctc catggcgcgc aggcgcatca cgccagacgt cgtttcctcc gacgccccgc
     2341 gcacgcgcgg gaggagatcg cggcgttgct ggtggagccg cgtgaccaat tccgcgccgt
     2401 cgtccacgat caggtccggc tcgaaatcga gtgtcgcgag cagatgctcg tcgaaggttc
     2461 tcgggtcggc ggcgtggatc gcgtggacgg tcacgccgcg ggcgacgagc gccgcgcaca
     2521 cgtcgtcctg cgtggagtag ggattgctcc cggtcgccgc gacttgcgcg ccggcctcgt
     2581 ggaaaagcca ggcgaggtag gcagtcttcg cctcgaggtg cacggcgatc gcgattcgca
     2641 ggccgcgcag ggcgccgtca gagaggcgct ccgcggcgaa agagttgagc gtcggcgcat
     2701 gctgagcgac ccactggatc ttgcgctccc cctccggggc gagggagatg tcgcgaatcg
     2761 tggacgcagg caacgtcaat cctccaaccg agcgccaccg aacaccgggc gccctccgag
     2821 cgagatccga aaggcggcga tggcgatcgc cgccatgagc gcgacggcga acagccccac
     2881 gccgaaatac cagcttgacg tatcggtcgt aaaggggaac tgattgacga ggaagtcgaa
     2941 atataaggtc gtaatcgcgg cgaccagccc aaaacggatg aacacaagcg caatgccgcc
     3001 aacgacgatg attcccgtga cggtagccag caacttcacg tcgctctcga gcacccggcc
     3061 gatccccaga aggagaacga aggccgtcgc ggccgcccat tcgctccgaa gcagcattcg
     3121 gagcagaaag aggaccaaga gcattccgaa gccgataaag atcgcatccg gcaacatcga
     3181 gaccgtttcg gcgatgacct tacgggtgcc gagaagcagc ggcggcagaa cgtcttgagg
     3241 acgaggcgga actccgagtc gtgggcccac aactatcgcc aggcgactga ccgttccgat
     3301 aacactcccc atgatgcaac cgatgaggac gtcgcgtccg acc
//
LOCUS       DASWAV010000367        11631 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U2665
            k141_180585, whole genome shotgun sequence.
ACCESSION   DASWAV010000367 DASWAV010000000
VERSION     DASWAV010000367.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960316
            Sequence Read Archive: SRR10260007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 11631)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11631)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:47:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,929
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11631
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_180585"
                     /isolate="SMAG_U2665"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.36444 N 132.31889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..195)
                     /locus_tag="VF958_07655"
     CDS             complement(<1..195)
                     /locus_tag="VF958_07655"
                     /inference="COORDINATES: protein motif:HMM:NF012239.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HEX9149020.1"
                     /translation="MGKEKFDRSKPHVNIGTIGHVDHGKTTLTSAITLVLAKKGLAQA
                     KAYDQIDNAPEEKARGITINT"
     gene            complement(216..291)
                     /locus_tag="VF958_07660"
     tRNA            complement(216..291)
                     /locus_tag="VF958_07660"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(256..258),aa:Thr,seq:ggt)
     gene            complement(317..391)
                     /locus_tag="VF958_07665"
     tRNA            complement(317..391)
                     /locus_tag="VF958_07665"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(357..359),aa:Gly,seq:tcc)
     gene            complement(478..564)
                     /locus_tag="VF958_07670"
     tRNA            complement(478..564)
                     /locus_tag="VF958_07670"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(528..530),aa:Tyr,seq:gta)
     gene            complement(620..694)
                     /locus_tag="VF958_07675"
     tRNA            complement(620..694)
                     /locus_tag="VF958_07675"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(660..662),aa:Thr,seq:tgt)
     gene            complement(875..1603)
                     /locus_tag="VF958_07680"
     CDS             complement(875..1603)
                     /locus_tag="VF958_07680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011197229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HEX9149021.1"
                     /translation="MILSRLHPIEEAVRSRAAEIEWVLLDSARRDRRIGELARLCRER
                     HVPVRFGTREALDRVARNHQGALARMAARGYASEEEALAGQTGRRLVIVLDGIEDPQN
                     LGATLRVADGVGAAVVLPERGSAPLSEAVARSSAGAIERVPVARVGNLRRFLDRLKEE
                     GFRVVGLDTAGTDLYREDLTGDLAIVLGAEGRGMRRLVREGCDVVARLPMRGALASLN
                     VATAAAAVGYEAVRQRDFSGRGAD"
     gene            complement(1600..2073)
                     /gene="ispF"
                     /locus_tag="VF958_07685"
     CDS             complement(1600..2073)
                     /gene="ispF"
                     /locus_tag="VF958_07685"
                     /EC_number="4.6.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008172883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008685 - 2-C-methyl-D-erythritol
                     2,4-cyclodiphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 2,4-cyclodiphosphate
                     synthase"
                     /protein_id="HEX9149022.1"
                     /translation="MRVGVGFDAHPFVDGRPLRLGGIAIAHPKGLAGHSDGDALLHAV
                     ADALLGAAGRGTLGEHFPDGSDAWRGADSAVLLSRAAGLVGEAGFRVGNLDAVVIAEA
                     PKIAPHAAAIRARVAEILGVAPGRVSVRGTSANGLGFTGRGEGIAAMAVALLEEL"
     gene            complement(2070..2753)
                     /gene="ispD"
                     /locus_tag="VF958_07690"
     CDS             complement(2070..2753)
                     /gene="ispD"
                     /locus_tag="VF958_07690"
                     /EC_number="2.7.7.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012933166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050518 - 2-C-methyl-D-erythritol
                     4-phosphate cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HEX9149023.1"
                     /translation="MANLLIIAAAGMGSRLGRSEPKALVPLLGRPLICWTLDAFAAIP
                     FARRRVAVPPGRENRFEAAIGGSAGIVAGGETRAASVRRAFESLNADPADLVCVHDAA
                     RPFVTPGETRSVLDAAAGTGAAIAATPVVDTLKRVERNRIVSTVDREGLFAAATPQVF
                     RADLLARAFAAAGDATDEAVLVEALGVTVAVVAVSRLSFKVTTPEDLEMAEALIRSRT
                     PESGKRKAK"
     gene            complement(2761..3315)
                     /gene="frr"
                     /locus_tag="VF958_07695"
     CDS             complement(2761..3315)
                     /gene="frr"
                     /locus_tag="VF958_07695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009611042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HEX9149024.1"
                     /translation="MRQELKDVEKRMSDAVQALKEKFKTLRTGRANLGLLDGIVVTAY
                     GAEVPINQVANLSVPEPTLIVAAPWDPKMAPEIERAIRKSDLGLNPQSDGKVVRIPIP
                     TLTEERRKDLAKKAHHMAEEARVEIRRFRHEGNDRLKKMYRDKEISEDDERRGLEETQ
                     KLTDKKIAEVDEVLKGKEKEILAV"
     gene            complement(3312..4046)
                     /gene="pyrH"
                     /locus_tag="VF958_07700"
     CDS             complement(3312..4046)
                     /gene="pyrH"
                     /locus_tag="VF958_07700"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005998099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033862 - UMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HEX9149025.1"
                     /translation="MQAQPVYQRVLLKLSGEALMGDRPFGIDPDVVARIADEVREVHL
                     LGVEMAIVVGGGNIIRGMAAAAQGIDRVAGDHMGLLATVINGLALLDALEKRHVTTRL
                     MSAIDVRQMAEPFIRRRALRHLEKGRVVLFAGGTGNPFFTTDSAAALRANEIKAEVLL
                     KATRVDGVYDANPERDPAAKFLRTVSYRQALESGLQVMDAAAIALCMDNGIPIKVFNM
                     RVSGNILRVVMGEDIGSLVSTESEVS"
     gene            complement(4114..5001)
                     /gene="tsf"
                     /locus_tag="VF958_07705"
     CDS             complement(4114..5001)
                     /gene="tsf"
                     /locus_tag="VF958_07705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009646772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor Ts"
                     /protein_id="HEX9149026.1"
                     /translation="MEISSKQVAELRAETGVGMMDCKKALVEASGDFEEAKKILRKKG
                     LAAAARKADRMASEGLVLARVTPKAAVLVEVNCETDFVARTSDFRSLADGLADQIAAH
                     DAFGETRAGDAGELALLPSTDRSTETVADAISHLVAKLGENIAIRRFARLVPRPEERF
                     ASYVHGNGRIGVLVGIRGGDLDLGKDIAMHVAASDPRFAARSEVTHEMLETEREIARA
                     QATQAGKPANVVDRIAAGKVEKFYQETVLIEQAFVKDPEKTVGQVVVERGGKEALDVR
                     FVRFKLGEAPVRSEGKAEA"
     gene            complement(5016..5792)
                     /gene="rpsB"
                     /locus_tag="VF958_07710"
     CDS             complement(5016..5792)
                     /gene="rpsB"
                     /locus_tag="VF958_07710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020706765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HEX9149027.1"
                     /translation="MVSISMKELLEAGVHFGHQTKRWNPKMKKFIFGKRNGIYIIDLQ
                     KTLKQFKDAAKFVTELAASGKNLLFVGTKRQAQDAIYEEANRCGMFFVNTRWLGGTLT
                     NFSTIRKSIHRLKEIEGVLAQEDNENLTKKERLRLEREKEKLQKNLTGIKEMDELPDA
                     LFVIDPKQEYIAVKEANILGIPVVAVVDTNCDPDVIDHVIPGNDDAIRAIRLFASRVA
                     DAVLEGLHAHEERAAEEAATAEERPRTPELSQTAVVADAS"
     gene            complement(5836..6231)
                     /gene="rpsI"
                     /locus_tag="VF958_07715"
     CDS             complement(5836..6231)
                     /gene="rpsI"
                     /locus_tag="VF958_07715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014098888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HEX9149028.1"
                     /translation="MTVEIQHRATGRRKTSMARVRLTPGTGAITVNARSLDDYFPNNV
                     LKMIIKQPLLSVEALEKFDIHVRVEGGGASGQAGAIRHGISRALTEYNAELRKKLKKE
                     GFLTRDPRMKERKKYGQRGARARFQFSKR"
     gene            complement(6228..6701)
                     /gene="rplM"
                     /locus_tag="VF958_07720"
     CDS             complement(6228..6701)
                     /gene="rplM"
                     /locus_tag="VF958_07720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006909970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HEX9149029.1"
                     /translation="MGSSTPMVSIKDVRHEWYVVDATNQVLGRLATRVARVLAGKHRP
                     GFVPYLDTGDFVIVTNADKVRLTGQKLDEKLYRHHTGFPGGLRESRAREVQSRHPERL
                     IEEAVRGMLPKSRLGRKQIRKLKVYRGATHPHDAQQPKPLAARTGAAARRTTEAN"
     gene            7001..7924
                     /locus_tag="VF958_07725"
     CDS             7001..7924
                     /locus_tag="VF958_07725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012040212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 5,10-methylenetetrahydrofolate
                     dehydrogenase/5,10-methenyltetrahydrofolate
                     cyclohydrolase"
                     /protein_id="HEX9149030.1"
                     /translation="MQLLEGAPVARRIRDEARAAAGRLEPAPRLVVILLGNDPASEAY
                     VSSKTRAAQEAGVLAETVRLPTGSPPETLLDSVAAANRDPAVDAVLVQMPLEPGHDAA
                     RVIDSLDPHKDVDGLHPENVGLLHQGRPRFVPCTPAGIMALLEQSGVTFAGQRAVILG
                     RSALVGRPLAALLTARDATVTVCHSKTRDIAAVCRQAEILVAAIGRPGFVTADFVRPG
                     ATVVDVGINRLESLTEAPENLQRSARIANAIASKGRAIVGDVDFDDVSPVAARITPVP
                     GGVGPLTVAMLIHNTVRAARMRRGAEDTKPS"
     gene            7962..8408
                     /locus_tag="VF958_07730"
     CDS             7962..8408
                     /locus_tag="VF958_07730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9149031.1"
                     /translation="MNSITWLDPTGFVGVVAAYTKPICKDIAANKPPSTKPSGALETL
                     METPQALEMPLQRQVSQKKTDYDALTAEITKTDAAYKAAPATAQPMLSAQLAALRGRQ
                     ASAKADLDRVSAAYQKFEALKPSLPKAAAPVAPRRTPLARRPRPVD"
     gene            8501..8788
                     /locus_tag="VF958_07735"
     CDS             8501..8788
                     /locus_tag="VF958_07735"
                     /inference="COORDINATES: protein motif:HMM:NF015534.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Type 1 glutamine amidotransferase-like
                     domain-containing protein"
                     /protein_id="HEX9149032.1"
                     /translation="MRVLLISSSNVHGTGYLDHAEPEIRDFLGATRRVLFFPWALRDL
                     DGYASKVRECLGRMDFEVGSAHDAPDPVRAVEDAEAVFVGAWYEREIIRQN"
     gene            8887..9273
                     /locus_tag="VF958_07740"
     CDS             8887..9273
                     /locus_tag="VF958_07740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007416350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HEX9149033.1"
                     /translation="MTKEPPRPTPAELEILGVLWEFGPSTVREVQEALESRRPTGYTT
                     ALKLLQIMTEKGLVRRDATERAHRYAPKLPRERTEQQMVGDLVDRAFGGSASRLVMRA
                     LSSRPASTDELERIRQLLDDIEREKR"
     gene            9270..11321
                     /locus_tag="VF958_07745"
     CDS             9270..11321
                     /locus_tag="VF958_07745"
                     /inference="COORDINATES: protein motif:HMM:NF017390.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M56 family metallopeptidase"
                     /protein_id="HEX9149034.1"
                     /translation="MTASALFAGQFFSALGWALFHFLWQGALVAGLFAALGWFLRKRT
                     PALKYALACGALATMIALPIATACSLATHARDRADSGMPPAEPPAPMLVSAADGASNA
                     IAGVDRLLPATLRNRIEALRPWTLSAWFAGVLLLSIRFLGGLAAAHRISRRGTRPAPA
                     AWEETLARIATRMAIARPVRLLESTIVKVPTAIGAFRPVILFPATVFLGLPTRGLEAL
                     LAHELAHVRRHDYLVNVLQTAAETLLFYHPAVWWVSARVRAEREQCCDDMAIAVTGDA
                     RTYARALIRLEEMRGAAPTLGVAAAGGNLWKRIFRLLSQRPPETNRSAGWLAGALALT
                     TMIVFGAAARPGPTSAWATDSIGVPLPDAIVRAVPAQTGPPQLTARAEDAAAPEEAPA
                     AELPESVPEPEEQAIAETAYESEPPEAPVPPEPPESRTLDDDEIDALRRHGVTPDYVR
                     AIAALGYERSDPDDILSLKIHGVTPKDIADLNAIFGRLPLDEHVSFKIHGVDAAYVEK
                     LQAGGDRHLSADDAISAKIHGVAPDDAEAWNRLGYGSVSLDDLVAARIHGASPEFAAE
                     VRSLGFSGLTLDDLVAFRIHGVTGDFLRQMKSLGYSPPAFSDDDAVAFRIHGVTPEFV
                     RQLRSLGYSDASPDDLVGLRVHGISTEDVRRMNARAGRRIPLEDVIDRHDCGNEKDDE
                     S"
     gene            11311..11514
                     /locus_tag="VF958_07750"
     CDS             11311..11514
                     /locus_tag="VF958_07750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9149035.1"
                     /translation="MRADVKERRRAITALAVAAAVYLVAPSRPALAAIEGRWIAEFDR
                     PDDRVQLTMERRSANHRSTNSSS"
BASE COUNT         1812 a         4112 c         3691 g         2016 t
ORIGIN      
        1 cgtgttgatc gtgatccccc gcgccttctc ctccggcgcg ttgtcgatct ggtcgtacgc
       61 cttggcctgc gccaacccct tcttcgccaa caccagcgtg atcgcagagg tcaacgtcgt
      121 cttcccgtga tccacgtgcc caatcgtccc aatgttgacg tgcggcttgc tgcgatcgaa
      181 cttttccttg cccatctctc tccctaacgt tcttatggag cccacgacca gaattgaact
      241 ggtgaccccg tccttaccaa ggacgtgctc taccgactga gctacgtggg cgcggtacca
      301 atctctcccg tccgtatgga gcgggagacg ggattcgaac ccgcgaccaa cagcttggaa
      361 ggctgtgact ctacccctga gttactcccg cgttttttcg tgaaacggga aacggtaaac
      421 gggaaacgac cgcctcgttt tccgttttgc gttttccgtt cttccgtcct ccgttactgg
      481 tgggcaggga aggattcgaa cctccgtagc gcgaagcgcg gcagatttac agtctgctgc
      541 cattaaccac tcggccacct gcccgagctc aaagggaaac ttgaaaaccg aaccgccccg
      601 ccctttgtcg accctctcgt ggagccggcg atgggactcg aacccgcaac cttccgatta
      661 caaatcggat gctctaccgt tgagctacgc cggcaacgat gccattcgga gaacttcgag
      721 agtccccggg ccctccggcc ggccttcaaa gagcagaccc atcccgacag cgcgaaccgc
      781 gaagtctagc acccgaagat tcgtcaagca aggcccggag tgaccgtatt tccggggatt
      841 ttctcctgca cggggcctgg cccgatcttg cccttcaatc ggcgccgcgg ccggaaaaat
      901 cccgttggcg gaccgcctcg tagccgacgg cggcagccgc ggtcgcgacg ttcagggaag
      961 cgagcgcccc cctcatcggc aggcgggcca cgacgtcgca cccctcccga acgagccgac
     1021 gcatcccgcg gccctcggcg ccgagcacga tcgccaggtc gcccgtcagg tcctcgcggt
     1081 agaggtcggt acccgcggtg tccagtccga ccacgcggaa cccctcctcc ttcaagcggt
     1141 ccaggaatcg gcggaggttg ccgacgcggg ccaccggcac gcgttcgatt gcacccgccg
     1201 aacttcgggc aacggcttcc gacaggggtg ccgagccgcg ctccggcagc acgaccgcgg
     1261 cccccacgcc atccgccacg cgaagcgtcg ctcccaggtt ctgcggatcc tcgatcccgt
     1321 cgagaacgat cacgagacgc cgccccgtct ggccggcgag cgcctcttcc tcggacgcat
     1381 aaccgcgcgc cgccattctc gcgagcgctc cctggtggtt ccgcgccacc cgatcgagcg
     1441 cctcccgtgt tccgaagcgc accggcacgt gccgctcgcg gcacagtcgg gcgagctcgc
     1501 cgatgcggcg gtcccggcgg gccgagtcca ggagcaccca ctcgatctcg gccgcccgcg
     1561 accgaaccgc ctcctcgatc ggatggaggc gcgacaggat caaagctctt cgaggagcgc
     1621 caccgccatc gcggcgattc cctcgcctcg cccggtgaaa ccgagaccgt tggcgctggt
     1681 cccccggacg gagaccctgc ccggagcaac gcccaggatc tccgccacgc gcgcccggat
     1741 ggcggcagcg tgcggagcga tcttcggcgc ctcggcgatc acgacggcgt ccaggtttcc
     1801 gacacggaat cccgcctcgc cgacgagccc cgccgcccgg gagagcaaga ccgcgctatc
     1861 cgcgccgcgc caggcatccg agccgtccgg gaagtgctcg ccgagcgttc cgcgtcccgc
     1921 cgccccgagt agagcatcgg cgaccgcatg gagcaacgcg tccccgtcgg agtggccggc
     1981 gagccccttc gggtgcgcga tcgcgatccc gccgagccgg agagggcgac cgtcgacgaa
     2041 cggatgcgca tcgaatccga ctcccaccct catttcgcct tccgttttcc gctctccggc
     2101 gtccggcttc gaatcagggc ttcggccatc tcgaggtctt cgggtgtcgt gaccttgaaa
     2161 gagagccgcg acacggcaac gacggcaacg gtgaccccga gcgcctccac gagcaccgct
     2221 tcgtccgtag cgtccccggc cgccgcgaac gcgcgtgcga gcagatccgc ccggaagacc
     2281 tgcggcgtcg ccgccgcgaa gagtccttcc cggtcgactg tcgaaacgat ccggttcctc
     2341 tccaccctct tcagcgtgtc cacgacgggg gtcgcggcga tcgcggcgcc cgttcctgcg
     2401 gcggcgtcga gcacggaacg ggtctctcca ggggtgacga acggcctcgc cgcatcgtga
     2461 acgcagacga ggtccgccgg gtcggcgttc agagactcga acgcccggcg caccgacgcc
     2521 gcccgggtct cgccgccggc gacgattccg gcgcttcccc cgattgcggc ctcgaaccgg
     2581 ttctcccgtc cgggaggtac ggcgacccgc cgacgggcga agggaatggc ggcgaacgcg
     2641 tccagggtcc agcagatgag agggcgcccg agaagcggaa ccagcgcttt cggctcgcta
     2701 cgaccgagtc ggctccccat accggccgcg gcgatgatca ggaggttggc cacttgtggc
     2761 tcagacggcc aggatctcct tctccttgcc cttcaggacc tcgtcgacct ccgcgatctt
     2821 tttatccgtc agcttctggg tctcctcgag accgcggcgc tcgtcgtcct cggagatctc
     2881 cttgtcgcga tacatcttct tcaaccggtc gttgccctca tggcggaatc gccggatctc
     2941 gacgcgcgcc tcctcggcca tgtggtgagc cttcttcgcg aggtctttgc gccgctcttc
     3001 ggtgagagtc ggtatcggaa tgcggacgac cttgccgtcc gattgcgggt tcagacccag
     3061 gtccgacttc cgaatcgcgc gctcgatctc cggcgccatc ttcggatccc agggcgcggc
     3121 gacgatgagc gtgggctcgg gtaccgagag attcgccacc tggttgatcg gaacctccgc
     3181 gccgtacgcg gtgacgacaa tgccgtcgag caggccgagg ttcgcacggc cggtccggag
     3241 tgtcttgaac ttttccttca gggcctgcac ggcgtcgctc atccgctttt cgacgtcctt
     3301 caactcctgt ctcatgacac ctcgctctcg gtcgatacca gggaaccgat gtcttctccc
     3361 atcacgacgc gcaggatgtt ccccgaaacg cgcatgttga acaccttgat cggaatcccg
     3421 ttgtccatgc agagagcgat cgccgcggca tccatgacct gcaggccgga ctcgagcgcc
     3481 tggcggtagg agaccgtcct caggaacttc gccgcggggt cgcgctccgg attcgcgtcg
     3541 tagacgccgt cgactcgcgt cgccttcagg aggacctccg ccttgatctc gttcgcccgg
     3601 agcgccgccg cggagtcggt cgtgaagaag gggtttccgg tccctccggc gaagaggacc
     3661 acgcgcccct tttccagatg gcgaagcgca cggcggcgga tgaacggctc cgccatctga
     3721 cgcacgtcga tcgcggacat gaggcgcgtc gtgacgtgcc gcttctcgag ggcgtccagg
     3781 agcgcgagac cgttgatgac ggtggcgagc agacccatgt ggtcgccggc gacgcggtcg
     3841 atgccctggg cggccgcggc cattccgcgg atgatgttcc cccctccgac gacgatcgcc
     3901 atctcgacgc cgagcaggtg cacttcgcgg acctcgtcgg cgatgcgggc gacgacgtcg
     3961 gggtcgattc cgaacggccg gtcgcccatg agcgcctcgc cggagagctt caggagcact
     4021 ctctggtaga cgggttgggc ttgcattggc gggattatcc ccgacgagtt cacgagtcca
     4081 aagtccaatg tcggacggac tcggacctcc gactcaggcc tcggctttcc cctccgaccg
     4141 gaccggcgcc tcgccgagct tgaacctgac gaacctcacg tcgagcgctt ccttgccgcc
     4201 gcgctcgacg acgacctgcc cgaccgtctt ctccggatcc ttgacgaacg cctgctcgat
     4261 caggaccgtc tcctggtaga acttttccac cttgccggcg gcaatgcggt cgacgacgtt
     4321 cgcgggcttg ccggcctggg tggcctgcgc ccgcgcgatc tcccgttccg tctccagcat
     4381 ctcgtgagtc acctcggagc gcgccgcgaa tcgcggatcc gacgccgcca cgtgcatcgc
     4441 gatgtccttg ccgaggtcga ggtctccgcc tcggatcccg acgaggacgc cgatccgccc
     4501 gttgccgtgc acgtagctcg cgaaccgctc ttcgggacgc gggacgagcc gcgcgaaccg
     4561 gcggatcgcg atgttctcgc cgagctttgc gacgaggtga gagatcgcgt cggcgaccgt
     4621 ctccgtcgag cggtccgtcg acgggaggag ggcgagctcg cccgcgtcac ccgctcgcgt
     4681 ctccccgaac gcgtcgtgcg ccgcgatctg atcggcgagg ccgtcggcga gactgcggaa
     4741 atcggaggtg cgggcaacga agtctgtctc gcagttcacc tcgacgagaa cggcggcctt
     4801 gggcgtcacg cgcgccagga cgaggccttc cgaggccatc cggtcggcct tccgagctgc
     4861 ggcggcgagc cctttcttgc gaaggatctt cttggcctcc tcgaagtcgc cgctcgcctc
     4921 gacgagagct ttcttgcaat ccatcatgcc gacgccggtc tcggcgcgca gctcggctac
     4981 ctgtttcgaa ctgatttcca tcttcagagg actggttacg aggcgtccgc gacgacggcc
     5041 gtctgcgaaa gctccggcgt acgcggccgc tcttccgccg tcgccgcttc ctcggccgcc
     5101 cgctcttcgt gggcgtgaag cccctcgagc acggcgtcgg cgacccgcga cgcgaaaagg
     5161 cggatcgccc ggatcgcgtc gtcgttgccc gggatgacgt ggtcgatgac gtccgggtcg
     5221 caattcgtgt ccacgacggc gacaaccgga attccgagga tgttcgcttc cttgaccgcg
     5281 atgtattcct gcttcggatc gatcacgaag agtgcatcgg gaagctcgtc catttccttg
     5341 atgcccgtca ggttcttctg gagcttttcc ttctccctct cgaggcggag gcgttccttc
     5401 ttggtcaggt tctcgttgtc ttcctgagcg agaacccctt cgatctcttt caggcggtgg
     5461 atcgacttgc ggatcgtcga gaaattcgtc agggtcccgc ccagccagcg cgtgttgacg
     5521 aagaacatcc cgcagcggtt cgcctcttcg tagatcgcgt cctgcgcctg gcgcttggtg
     5581 ccgacgaaca ggagattctt tccggaggcg gcgagctccg tcacgaattt cgcggcatcc
     5641 ttgaactgct tcagggtttt ctgcaggtcg atgatgtaaa tgccgttgcg cttgccgaag
     5701 atgaatttct tcatcttcgg gttccaccgc tttgtctggt gcccgaagtg aacccccgct
     5761 tcgagcagct ctttcattga gatcgagacc aggaaacacc tccctttccg ccggcttcgc
     5821 cggccagtcg atccttcagc gcttgctgaa ctggaaccgg gcgcgcgctc cgcgctgccc
     5881 gtacttcttt ctttccttca tccgcgggtc ccgcgtgaga aacccttcct tcttaagctt
     5941 cttccgcagc tcggcgttgt actccgtcag agcccgcgag atcccgtgcc ggatcgctcc
     6001 ggcctgtccg gacgccccgc ccccctcgac gcgcacgtgg atgtcgaact tctcgagagc
     6061 ctctaccgaa agcagcggct gcttgatgat catcttcagc acgttgttgg ggaagtaatc
     6121 gtcgagggac cgcgcgttga ccgtgatggc tccggtgccg ggggtgagcc ggacgcgcgc
     6181 catggaagtc ttgcggcgac cggtggcccg atgctggatt tcgaccgtca atttgcctcc
     6241 gtggtgcggc gagccgcggc gcccgtcctc gcggcgagcg gcttgggctg ctgtgcgtcg
     6301 tgcgggtgag tggctccgcg gtagaccttc agcttgcgga tctgcttgcg gccgagtcgc
     6361 gatttcggaa gcatcccacg gaccgcctct tcgatcagcc gctcgggatg ccgtgactgg
     6421 acctcacgcg cgcgactctc ccgcaggcct ccgggaaacc ccgtgtgatg acggtacaac
     6481 ttctcatcga gcttctggcc ggtgagccgg accttgtccg cgttcgtgac gatcacgaag
     6541 tcgccggtgt cgagataagg aacgaacccg gggcgatgtt tgccggccag cacgcgcgcg
     6601 acgcgcgtgg cgaggcgccc cagcacctgg ttcgtggcgt ccacgacgta ccattcgtgg
     6661 cgcacgtctt tgatcgaaac cataggcgta ctcgagccca tcgaaactcc aaaaagcctt
     6721 tcaatcggga cgcgcgaggc ctctcggccg cgcgtcgagg acggacaaag cccttccagg
     6781 gggcgcgtaa ggatacgcat gggagggggg gctgtcaaac cgtcgcgctc gctcggccgg
     6841 gagttgcttc gcaccggcgg gcggctcgcc agacccggct cgaccgcccg cctactcccg
     6901 ccctcgctcc cgctcccgcg gcgggaggct cctccctcga ttacctcgga gactcctccc
     6961 gccgcgaaca gagccagagc caccctgata cttttccacg gtgcaactcc tcgagggagc
     7021 tccggtcgcc aggcgcatcc gggacgaagc ccgggctgcc gcgggccggc tcgagccggc
     7081 gccccgactg gtcgtcatcc tcctcggcaa cgacccggct tccgaggctt acgtctcttc
     7141 gaaaacgcgc gcggcgcaag aggccggagt tctcgcggag acggtgcgcc tccccacggg
     7201 ctctccgccc gagacactcc tcgactcggt cgcggccgcg aaccgcgacc cggcggtcga
     7261 cgcggtgctc gtccagatgc cgctcgagcc gggccacgac gccgcgcgcg tgatcgattc
     7321 gctcgacccc cacaaggacg tcgacggtct tcatccggag aacgtcggcc tcctccacca
     7381 gggacggccg cgcttcgtcc cgtgcacgcc cgcaggcatc atggcgttgc tcgaacaatc
     7441 cggcgtgacg tttgccgggc agcgggcggt gatcctcggt cggagcgcgc tcgtcggccg
     7501 gcccctcgcg gcgctcctga ccgcgcgcga cgcaacggtc acggtgtgcc attcgaagac
     7561 ccgggacatc gccgcggtct gccgccaggc cgagatcctc gtcgcggcga ttggccggcc
     7621 ggggttcgta acggccgatt tcgtgcggcc cggcgcgacc gtcgtcgacg tcgggatcaa
     7681 tcggctcgaa tcgctcacgg aggcaccgga gaatctccag cgctccgccc gaatcgcgaa
     7741 cgcgatcgca tcgaagggac gcgcgatcgt cggcgacgtc gacttcgacg acgtttcccc
     7801 cgtcgcagcg cggatcacgc cggtgcccgg aggcgtcgga ccgctcaccg tggcgatgct
     7861 gattcacaac accgtgcgcg cggcccggat gcggcgcggc gcggaggaca cgaagccaag
     7921 ctaagacttc cgagaagaac cacgcgatcg cgcagataac gttgaactcg atcacctggc
     7981 tggacccgac cggctttgtc ggcgttgtcg ctgcttacac gaagcccatc tgcaaggaca
     8041 tcgccgcgaa caagccgcca tcgacgaagc cgagtggggc tctggagacg ttgatggaaa
     8101 cgccgcaggc tctggaaatg ccgctccaga ggcaggtatc tcagaaaaaa accgattacg
     8161 acgctctgac cgcggagatc accaagactg acgcggcgta caaggccgcc cccgcaacgg
     8221 cgcagccgat gttgtcagct cagctggccg cgcttcgcgg ccgacaggcg tccgcgaagg
     8281 ccgatctcga ccgagtgtcc gcggcttacc agaagttcga ggcactgaag ccgagcctgc
     8341 ccaaggcagc ggctccggtc gcaccacgtc gcacaccact tgcgcggaga ccgaggcccg
     8401 tcgattgaga gcctgacgtg agcatctgag ggagggcgtc gggaggaaga acccgggaac
     8461 tggacgaaag aaccggtagg gtggttgcgt tgaggtgaac gtgcgcgttc tcctcatcag
     8521 cagctcgaac gtgcacggca cgggttatct ggaccacgcc gagccggaga tccgggactt
     8581 cctcggggcg acccggcgtg tcctcttctt tccgtgggcg ctccgggatc tggacggata
     8641 cgcgtcaaag gtccgggagt gcctgggccg gatggacttc gaggtcggct cggcgcacga
     8701 cgcgccggat ccggtccgcg ccgtggaaga cgccgaagcc gtgttcgtcg gcgcttggta
     8761 tgagagagaa ataattcggc agaattagct agacgcctaa taaaattcgg agcggcacgg
     8821 gcccgcccag gaacctctcc atcttacgaa aaccttcgta ttactcttgc gtatccggag
     8881 gtcaggatga cgaaggaacc cccgcgtccc acccccgccg agctcgagat cctcggggtc
     8941 ctctgggagt tcggcccgtc cactgtccgc gaggtgcagg aggcgctcga gtcccggcgg
     9001 ccgaccggtt acacgaccgc cctgaagctt ctgcagatca tgacggaaaa ggggctggtg
     9061 cgccgcgacg ccacggaacg cgcgcaccga tacgccccga agctgccgcg cgaacggacc
     9121 gagcagcaga tggtcggcga cctcgtcgac cgcgccttcg gcggctcggc gtcccgcctg
     9181 gtgatgcggg ctctttcttc gcggccggcg tcgaccgacg agctcgaacg gattcgccag
     9241 ctcctcgacg acatcgaaag ggaaaaacga tgacggcttc cgcgcttttc gccgggcagt
     9301 tcttttcggc tctcggttgg gcgctcttcc acttcctgtg gcagggagcc ctcgtcgccg
     9361 gcctcttcgc ggcactcggc tggttcctgc gaaagcggac tccggcgctc aagtacgcac
     9421 tcgcctgcgg cgctctcgcg acgatgattg cactgcccat cgcgactgcg tgttcgctcg
     9481 cgacccacgc ccgcgaccga gcggattccg gcatgccccc ggcggaacct ccggcgccga
     9541 tgctcgtgtc ggccgcggac ggcgcttcga acgcgatcgc cggggtcgat cggctccttc
     9601 ctgcgacgct ccgcaaccga atcgaggcgc tgcggccgtg gacgctttcg gcctggttcg
     9661 ccggagtcct gctcctctcg attcgcttct tgggcggcct cgccgccgcc catcggatca
     9721 gccggcgggg aacgcgcccg gcgcccgcgg cgtgggagga gactctcgcg cgcatcgcca
     9781 cgcgaatggc gatcgcgcgg ccggtccgct tgctcgagtc gactatcgtg aaggtgccga
     9841 ccgcgattgg cgctttccgc ccggtcatct tgtttccggc gacggtcttc ctgggactcc
     9901 cgacgcgagg actcgaggct ctcctcgcgc acgagctcgc ccacgtgcgc cggcacgatt
     9961 acttggtcaa cgttctccag accgccgccg aaacgctcct cttctaccac cccgccgtct
    10021 ggtgggtctc cgcgcgggtg cgcgccgagc gcgagcagtg ctgcgacgac atggcgatcg
    10081 cggtgacggg cgacgcccgg acgtatgcac gggcgctcat acgcctcgag gaaatgcgcg
    10141 gcgcggcacc gacgctcggg gtcgcggccg cgggcggaaa tctctggaag cgcattttcc
    10201 ggctcctttc gcagcgcccg ccggagacca accgttcggc cggctggctc gccggcgcgc
    10261 tcgcgctcac gacgatgatc gttttcggcg cggcggcccg cccggggccg acgagcgcgt
    10321 gggcgacgga ttcgatcggc gtgcctctgc ccgacgccat cgttcgcgca gtcccggctc
    10381 agaccgggcc cccgcagctc acggcgcgag cggaagacgc ggccgccccc gaagaggcgc
    10441 cggctgccga gcttccggag agcgtccccg agccggagga gcaggcgatc gcggaaaccg
    10501 cgtacgagtc tgagccgccc gaagcgccgg ttcctcccga gccgccggag tcccggacgc
    10561 tcgacgacga cgagatcgac gcgctccgcc gacacggcgt cacccccgac tacgtccgcg
    10621 ccatcgccgc cctcggctac gagaggtccg acccggacga catcctgtcg ctcaagatcc
    10681 acggcgtcac gccgaaagac atcgcggacc tgaacgcaat tttcggcaga ctcccgctcg
    10741 acgagcacgt ttcgttcaag atccacggcg tcgacgccgc ctacgtcgag aagctgcaag
    10801 cgggaggcga ccggcacctc tcggccgacg acgccatctc cgcgaagatc cacggcgtcg
    10861 cgccggacga cgcggaggcg tggaaccggc tgggatacgg gagcgtgtct ctcgacgatc
    10921 tcgtcgctgc acggatccat ggcgcgtccc ccgagttcgc cgcagaggtg cgctcgctcg
    10981 gcttctccgg cctgacactc gacgacctcg tcgcgttccg catccacggc gtgaccgggg
    11041 acttccttcg ccagatgaaa tcgctcgggt actctcctcc ggccttcagc gacgacgacg
    11101 cggttgcctt ccgcatccac ggcgtcacgc ccgagttcgt tcgccagctc cggtctctcg
    11161 gctactccga cgcctcgccg gacgacctcg ttggcctccg tgtccacggg atctcgaccg
    11221 aagacgttcg ccgcatgaac gcgcgcgccg gccggcggat ccccctcgaa gacgtgatcg
    11281 accgtcacga ctgcggaaat gagaaggacg atgagagctg acgtgaagga acggcgccgt
    11341 gcgatcacgg cgctcgccgt ggcggccgct gtctatctcg tcgcgccgtc acgccccgcg
    11401 ctcgccgcga tcgagggccg ctggattgct gagttcgacc gaccggacga ccgagtgcag
    11461 ctcaccatgg agcgccgctc cgcgaatcac cgctcgacga actcgagctc ctagtcccgt
    11521 tcggcgtttc gcggtctcgt ccggccgtcc ggctctcagc cggcgcccgc gcgtttcgag
    11581 ctcgcgcgcg acgccggaac gttgacgttc tcggggcagc tcgactcctc c
//
LOCUS       DASWAV010000368         4137 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U2665
            k141_699803, whole genome shotgun sequence.
ACCESSION   DASWAV010000368 DASWAV010000000
VERSION     DASWAV010000368.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960316
            Sequence Read Archive: SRR10260007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 4137)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4137)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:47:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,929
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4137
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_699803"
                     /isolate="SMAG_U2665"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.36444 N 132.31889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2232
                     /locus_tag="VF958_07755"
     CDS             <1..2232
                     /locus_tag="VF958_07755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009110224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HEX9149036.1"
                     /translation="VSVAVLYDRSASIRDSVNDVKFTLVLAICLVVLVIFLFLRNLSA
                     TVIPSLALPFSIVGTFALMYVLGYSLDNLSLMALTLSVGFVVDDAIVMLENIVRHIEG
                     GEGVLEAALNGSKEIGFTILSMTLSLTAVFIPILFMGGIVGRLFHEFAVTISVAILVS
                     GFVSLSLTPMLSSRFLRPDHGRKHGRLYDVSERYFDRMVGVYDRGLTWALARRRTTMA
                     VSALVLVATVFLFLKIPKGFLPSEDNSQIFGFTEAAQGISFRAMGEHQMAVAKEVQQD
                     PNILAFMSSYGSRGSINASNSGVVFIHLKPRKERTLSADGVIQELRPKLAKIPGIKVY
                     LQNPPPIRIGGSLTKSLYQYTLQDSDTTELYRYAPILEQKMRQLPGFQDVVSDLQIAN
                     PQVRVEIDRDKAAALHLTPQKIEDALYTAYSSRQISTIYAPNNEYQVIMELDPKYQGD
                     PAALSLLYVRNSDGQLIPLDTVARIDQNLGPLAITHAGQLPSVTLSFNLKPGESIGDA
                     VAAVNAAARETLPATITTNFQGTAQAFQSSFQGLGLLLLMAILVIYLVLGILYESFIH
                     PLTILSALPFAGFGALVTLLIFRTELSIYAFVGIIMLVGLVKKNGIMMVDFAIEAQRK
                     EGKDAQAAIHEACLVRFRPIMMTTMAALMGTLPIALGFGSGAEARRPLGLAVVGGLLF
                     SQLLTLFVTPVFFTYMEQFRERFGRRRGRGRPAKEAIETRAPAEEERVPAAAAMRSAT
                     PGA"
     gene            2248..3171
                     /locus_tag="VF958_07760"
     CDS             2248..3171
                     /locus_tag="VF958_07760"
                     /inference="COORDINATES: protein motif:HMM:NF013761.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline dehydrogenase family protein"
                     /protein_id="HEX9149037.1"
                     /translation="MSLLRSAFLAASESRWLRERAVKLPFVRRAVSKFMPGETFDDAL
                     AASGALGVGTVLTRLGENLRSADEAQGVARHYLEVLDQVTAEKLDVEISVKLTQLGLD
                     FSREECERHLRELAGRARQQGNWVWVDMESTAYTDVTLDIYRRLRAEFPNTGVCVQSY
                     LFRTAGDVEALITLGSGIRVVKGAYKEPPDQAFPKKEDVDENYFRLAERLLADDARAK
                     GVRAIFGTHDPALIGRIEEVGRSRGLAPADVEFQMLYGIRRPEQLRLAAAGYRFRVLI
                     SYGEAWFAWYMRRLAERPANVLFVVRSLFAR"
     gene            complement(3176..>4137)
                     /locus_tag="VF958_07765"
     CDS             complement(3176..>4137)
                     /locus_tag="VF958_07765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9149038.1"
                     /translation="PGYFRASFSVSDHGELVYATGTRESKAPLVWYDRAGKPIGGPVG
                     EAAEYVDLAISRDGKRVAAAISDPTTGVPDIWLLDFQRGTRTRFTFASSVRPIWSPDG
                     SRIVYASAGKPSAAGFSSVISVKPTGGEGKPEVVFNGEGQFTPTSWSEDSRFIALEVI
                     RPKSPTKTDVWILPLFGDRKPYPFLETEFSEEGASFSPDGRWISYLSDESGRGELYVT
                     SFPTRAGKWQVSNKGALVGGWARSGKEIVYVAADQNLMSVEVRADESGFDSGVPKVLF
                     KSPRILTGVAAPLADRFLLAPREEGSQGSSISLVSNWPALLRK"
BASE COUNT          695 a         1375 c         1314 g          753 t
ORIGIN      
        1 gtgagcgtcg ccgtcctcta cgaccggtcc gcctcgatcc gggactcggt gaacgacgtc
       61 aagttcaccc tggttctcgc catctgcctc gtggtcctgg tgatcttcct gttcctgcgg
      121 aacctctccg ccacggtgat ccccagcctc gccctgccgt tctccattgt cggcacgttc
      181 gcgctcatgt acgtcctcgg atacagcctg gacaacctct ccctcatggc gctgacgctg
      241 tccgtcgggt tcgtcgtgga cgacgcgatc gtcatgctcg agaacatcgt ccggcacatc
      301 gagggagggg aaggggttct cgaggcggct ctcaacgggt cgaaggagat cggcttcacg
      361 atcctgtcga tgacgctgtc gctgacggcg gtgttcatcc cgattctctt catgggcggc
      421 atcgtcggac gcctgttcca tgagttcgcc gtcacgatca gcgttgcgat cctcgtctcg
      481 ggtttcgttt cgctctcgct gacgccgatg ctctcgagcc gcttcctccg gcccgatcac
      541 ggcaggaagc acgggcggct ctacgatgtt tccgagcgct acttcgaccg gatggtgggg
      601 gtctacgacc ggggattgac gtgggcgtta gcccggcggc gtacgacgat ggcggtctcc
      661 gcgctggtcc tggtcgcgac ggtctttctc ttcctgaaga ttcccaaggg atttctgccg
      721 agcgaggaca acagccagat cttcgggttc accgaggccg cgcagggcat ctcgttccgc
      781 gcgatgggcg agcaccagat ggcggtcgcg aaggaggtcc agcaggatcc gaacatcctc
      841 gctttcatgt cgagctacgg ctcacggggc tcgatcaacg cgagcaactc gggggtcgta
      901 tttatccatt tgaagcctcg caaggagcga acgctctcgg ccgacggcgt gatccaggag
      961 ctgcgcccga agctcgcgaa gatcccgggc atcaaggtct atcttcagaa tcctcctccg
     1021 atccggatcg gaggaagcct gaccaagagc ctctaccagt acacgctgca ggactccgac
     1081 acgaccgagc tctaccgcta cgcgcccatc ctcgagcaga agatgcggca gcttcccgga
     1141 ttccaggacg tggtgagcga cctccagatc gcgaatccgc aggtccgcgt ggagatcgac
     1201 cgcgacaagg ccgccgcgct ccacctcacc ccgcagaaga tcgaggacgc gctctacacc
     1261 gcttacagtt cgcgccagat ctcgacgatc tatgcaccga acaacgaata tcaggtcatc
     1321 atggagctcg atccgaaata ccagggagac ccggcggcgc tgtcgctcct ctacgtgcgc
     1381 aactcggacg gccagctcat ccccctggac accgtggcga ggatcgatca gaacctcggg
     1441 cctctcgcga tcacgcacgc aggccagctt ccgtcggtca ctctctcctt caatctgaag
     1501 ccgggcgaat cgatcggcga cgccgtcgcc gcggtcaacg ccgcggcgag ggagacgctg
     1561 ccggcaacga ttacgacgaa cttccagggc acggctcagg cgttccagtc ctcgttccag
     1621 ggcctgggcc tgctgctgct gatggcgatt ctcgtcatct acctggttct cggaatcctg
     1681 tacgagagct tcatccaccc gctgacgatc ctctcggcgc tgccgtttgc cgggttcggc
     1741 gcgctcgtga ccctgctcat cttccgcacg gagctctcga tctacgcctt cgtcgggatc
     1801 atcatgctcg tcggtctcgt caagaagaac ggaatcatga tggtggactt cgcgatcgaa
     1861 gcgcagcgca aggagggaaa agacgcgcaa gccgcgattc acgaggcgtg tctcgtccgg
     1921 tttcgtccga tcatgatgac gacgatggcg gccctcatgg gaacgctgcc aatcgccctc
     1981 ggcttcggtt ccggtgccga agcacggcgc ccgctgggcc tcgcggtcgt cggcggcctt
     2041 ctcttctctc agctcctgac gctcttcgtg acgccggtct tcttcacgta catggagcag
     2101 ttccgtgagc ggttcggccg ccgccgcggc cggggacgcc cggcgaaaga agcgatcgag
     2161 acgcgcgctc cggcggaaga ggaacgggtt cccgcagcag cggcgatgcg ctcggcgacg
     2221 cccggcgcct gagcttccag actccgaatg agccttctgc gaagcgcctt cctcgcggcg
     2281 tcggagagcc ggtggctgcg cgagcgtgcc gtcaagctcc ccttcgtccg acgcgccgtc
     2341 tcgaagttca tgccgggcga gaccttcgac gatgcgctcg ccgcgtcggg cgcgctcggc
     2401 gtcggaaccg tgctgacgcg actcggcgaa aacctgcgca gcgcggacga ggcgcaaggc
     2461 gttgcgcggc actatctcga ggtcctcgac caggtcacgg cggagaagct cgacgtcgag
     2521 atctccgtca agttgaccca gctcgggctc gacttttcgc gggaggagtg cgagcggcat
     2581 ctgcgggagc tcgcgggacg tgcgcggcag caaggcaact gggtctgggt ggacatggag
     2641 tcgacggcgt acaccgacgt cacgctcgac atctatcgcc gcctccgcgc cgagtttcca
     2701 aacacggggg tttgcgtgca gtcgtacctg ttccgcaccg ccggagacgt cgaggcgctg
     2761 atcacgctgg ggtcgggaat tcgcgtcgtg aagggcgcat acaaggagcc gccggaccag
     2821 gcgttcccga agaaggagga cgtcgacgag aactacttcc gcctcgcgga gcgactcctc
     2881 gccgacgatg cgcgcgcgaa gggggttcgc gcgatcttcg ggacgcacga tccggcgctc
     2941 atcgggcgga tcgaagaggt cggccggtca cgcggactcg ctccagcgga cgtcgagttc
     3001 cagatgcttt atggaatccg ccgccccgag cagcttcgtc tcgcagcggc cggctaccga
     3061 tttcgcgtgc tgatcagcta cggcgaagcg tggttcgcgt ggtacatgcg gcggctcgcc
     3121 gaacgccccg cgaacgtgct gttcgtggtg aggagcctct tcgcccgctg aacgcctact
     3181 tcctcagcag cgccggccag ttcgagacga gcgagatcga ggacccctgc gacccctcct
     3241 cgcgcggagc cagcaggaat cgatcggcga gaggcgccgc aacgcccgtc agaatccgcg
     3301 ggctcttgaa gagcaccttc ggcacgccag agtcgaaccc ggactcgtcc gcgcgcactt
     3361 cgacggacat caggttctga tcggcggcca cgtagacgat ttctttgccc gatcgagccc
     3421 acccgccgac gagagcgcct ttgttcgaga cctgccattt gccggcccgg gtcgggaagg
     3481 acgtcacgta tagctcaccc ctccccgatt cgtcggagag gtacgagatc cagcgtccgt
     3541 cgggagagaa gctggcgccc tcttccgaga actccgtctc gaggaacgga tagggcttcc
     3601 ggtcgccgaa gagcgggaga atccagacgt cggttttcgt cgggctcttc ggccgaatga
     3661 cttccagggc gatgaaccgg ctgtcttcgg accatgacgt cggggtgaac tggccctctc
     3721 cgttgaagac gacttccggt ttgccctcgc ccccggtcgg tttcacggag atgacgctgc
     3781 tgaagccggc cgcgctcggc tttcccgcgc tcgcgtagac gatccggctg ccgtccggcg
     3841 accaaattgg ccggacgctc gacgcgaacg tgaaccgcgt cctggttccg cgctggaagt
     3901 cgagaagcca gatgtccggc accccggtcg tgggatcgct gatcgccgcc gcgaccctct
     3961 tcccgtcccg ggagatcgcg agatccacgt actccgcagc ttcgccgacg gggccgccga
     4021 tcggcttgcc cgctcggtcg taccagacga gcggggcctt cgactcacgc gtcccggtgg
     4081 cataaacgag ctcgccgtga tcggagaccg agaacgacgc gcggaaataa ccggggt
//
LOCUS       DASWAV010000369         1583 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U2665
            k141_540152, whole genome shotgun sequence.
ACCESSION   DASWAV010000369 DASWAV010000000
VERSION     DASWAV010000369.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960316
            Sequence Read Archive: SRR10260007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 1583)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1583)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:47:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,929
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1583
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_540152"
                     /isolate="SMAG_U2665"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.36444 N 132.31889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1583
                     /gene="uvrA"
                     /locus_tag="VF958_07770"
     CDS             <1..>1583
                     /gene="uvrA"
                     /locus_tag="VF958_07770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013425997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HEX9149039.1"
                     /translation="KAAREVILYGSGEDEIRFSWVAEKGEYHWKSSFEGLVPMIERRY
                     RESENPEHRADLGMYMSAEPCPACKGRRLKPEALAVKVGGRSVDEVAGETIAESISFF
                     DELTLTQRESEIAGKILKEIRDRLGFLANVGIGYLNLSRGAASLSGGESQRIRLATQI
                     GSKLMGVLYVLDEPSIGLHQRDNRRLIDTLLSMRDLGNTVIVVEHDEETILSADWVID
                     LGPGAGVHGGRLVAQGKPGDLSRFSQSLTARYLSGELEIPVPAVRRAGSGRSLVVRGA
                     KEHNLKSIDVRFPLGVITAVTGVSGSGKSTLVNDILYRALARALHGATDRPGRHDRIE
                     GLFEIDKVINIDQSPIGRTPRSNPATYTNVFNPIRELMSRTPEARARGYAPGRFSFNV
                     KGGRCEACAGDGQIKIEMHFLPDIYVTCDVCGGKRYNRETLQVHYKGKSIADILALTA
                     EQGLELFQNVPAIANICRTLVEVGLGYIQLGQSSTTLSGGEAQRVKLARELARRSTGR
                     TLYLLDEPTTGLHFDDIKK"
BASE COUNT          276 a          517 c          534 g          256 t
ORIGIN      
        1 cgaaagcggc ccgggaagtc atcctctacg gatcggggga agacgagatc cgcttctcgt
       61 gggtggccga gaagggcgag taccactgga agtcctcgtt cgagggcctg gtgccgatga
      121 tcgagcgccg ctatcgcgag agcgagaacc cggagcaccg cgccgacctc gggatgtaca
      181 tgtccgcgga gccgtgtccg gcgtgcaaag ggcgccgcct gaagccggaa gctctcgcag
      241 tgaaggtcgg cggccggtca gtcgacgagg tcgcgggcga gaccatcgct gagtcgatct
      301 cgttcttcga cgagctcact ctgacgcaga gggagagcga gatcgcgggg aagatcctga
      361 aggagatccg cgaccgcctc ggttttctcg cgaacgtcgg gatcgggtat ctgaacctct
      421 cccggggcgc ggcttctctc tcgggtgggg agtcccagcg gatccgcctg gcgacgcaga
      481 tcggttcgaa gctgatgggc gttctctacg tgctcgacga gccctcgatc ggcctccacc
      541 agcgcgacaa ccggcggctg atcgacacgc tcctttcgat gcgcgacctc ggcaacaccg
      601 tcatcgtcgt cgagcacgac gaggagacga tcctttccgc ggactgggtg atcgatctcg
      661 gccccggcgc cggcgtacac ggcggccgac tcgtcgctca gggaaagccg ggggatctct
      721 cgcgcttctc ccagtcgctg acggcacgct acctgtccgg agagctcgag attccggtcc
      781 ccgcggtccg gcgcgcgggc tcggggcgct cgctggtcgt gcgcggggcg aaggagcaca
      841 acttgaaatc gatcgacgtg cgtttcccgc tcggcgtgat cacggcggtg acgggcgtct
      901 ccggctcggg caagtcgacg ctcgtcaacg acatcctcta ccgtgccctc gcgcgagccc
      961 ttcacggcgc caccgaccgt ccgggccgcc acgaccgcat cgaagggctc ttcgagatcg
     1021 acaaggtgat caacatcgac cagtcgccga tcggccgcac gccccgatcg aaccccgcca
     1081 cgtacacgaa cgtgttcaac ccgatccggg agctcatgtc gcgcacgccc gaggcgcggg
     1141 cgcggggcta tgcgccgggg cggttttcct tcaacgtgaa gggcgggcgt tgcgaagcgt
     1201 gcgctggaga cggtcagatc aagatcgaga tgcacttcct tccggacatt tacgtcacgt
     1261 gcgacgtctg cggcgggaag cggtacaacc gcgagacgct gcaagttcac tacaagggca
     1321 aatcgatcgc ggacatcctc gcgttgacgg cggaacaggg tctcgagctc ttccagaacg
     1381 tgcccgcaat cgcgaacatc tgccgaaccc tcgtcgaggt gggtctcggg tacatccagc
     1441 tggggcagtc gtcgacgacg ctctcgggcg gggaggccca gcgcgtcaag ctcgcgcgag
     1501 agctcgcccg ccggtcgacg ggccggacgc tctatctgct cgacgagccg acgaccggcc
     1561 tgcacttcga tgacatcaag aag
//
LOCUS       DASWAV010000370         8327 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U2665
            k141_58353, whole genome shotgun sequence.
ACCESSION   DASWAV010000370 DASWAV010000000
VERSION     DASWAV010000370.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960316
            Sequence Read Archive: SRR10260007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 8327)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8327)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:47:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,929
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8327
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_58353"
                     /isolate="SMAG_U2665"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.36444 N 132.31889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..468)
                     /locus_tag="VF958_07775"
     CDS             complement(<1..468)
                     /locus_tag="VF958_07775"
                     /inference="COORDINATES: protein motif:HMM:NF014809.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit II transmembrane
                     domain-containing protein"
                     /protein_id="HEX9149040.1"
                     /translation="MKETFPLFPNAASTVAGRVDALYFYLIIVSVVFTLLIAASILYF
                     AIKYRRRSESELPRGVEGSLKLEIAWSVIPLVIALSFFFWGARVFFTINRPPPDALEV
                     SVVGKQWMWKVQHADGQREINELHVPVGRPVRLTMTSEDTIHSFYVPAFRIKRD"
     gene            complement(465..1295)
                     /locus_tag="VF958_07780"
     CDS             complement(465..1295)
                     /locus_tag="VF958_07780"
                     /inference="COORDINATES: protein motif:HMM:NF014667.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCO family protein"
                     /protein_id="HEX9149041.1"
                     /translation="MRTVAVVLAVLAISTALPAQPAPPAPDGRPPILRGVDIEQHLDR
                     PLPLDLEFKDETGRTVRLRDYFGKRPVVLVLAYYDCPMLCTQVLNGLVSAMGVLSFDV
                     GREYEVVTVSFDPRDDPSDARAKKEPYVARYGRTGGAAGWHFLTGEPPSIAALTNAVG
                     FRYAWDEKIGQFAHASAIYVATPDGRLARYFYGIEYAPRDLRLAIVEASQGKIGTPVD
                     KILLYCYHYDPATGRYGAVVMNMVRLGGVAFVLILSTFLFLMWRRERRLAANRAVEAR
                     "
     gene            complement(1292..1756)
                     /locus_tag="VF958_07785"
     CDS             complement(1292..1756)
                     /locus_tag="VF958_07785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9149042.1"
                     /translation="MAHTDPASPTPEAGHETSDVSISAIVKFGVGLAIVAALVLVAMW
                     GLLRSFQAGAAKRDQPVPPLVAASLRRTPREPRLEPDPLAPRVAAQAREDAILESYGW
                     VDRNGGIARIPIDRAMEILVERGLPPAKPMMPVMTPAPTPGPGKRETGNGKR"
     gene            complement(1743..2909)
                     /locus_tag="VF958_07790"
     CDS             complement(1743..2909)
                     /locus_tag="VF958_07790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9149043.1"
                     /translation="MAHVHGFERFQRPAFLVGAAALAICAVGGFFAPAQFFRSYLFAF
                     LFWTGLALGCMAVAMLNHVTGGAWGVAIRRPLESGTRTLPLLLLLFLPIVFGARRIYE
                     WADPATVARDPVLRHKSLYLNLPFFTGRALFYFAAWIALAYFLNRWSLEQDATGARPI
                     ARRLQLLSSAGLVAYGLTVTFASIDWAMSLEPHWFSTMYGVLFIAGQGLTAFAFIVVV
                     LVLLAGTPPLAGYVGQRHYHDLGKLMLAFVMFWAYVSFSQYLIIWAGNLPEEIPWYLR
                     RLQGGWGWVGIALIVFHFLLPFLLLLPASANRNPRVLAAVAGLVLFMRLIEVFWLTQP
                     AFAGGHFRLHWMDVLAPVGVGGLWLATFLWQLGKRPLLPMNDPEMLQAVAHGTH"
     gene            complement(2909..3547)
                     /locus_tag="VF958_07795"
     CDS             complement(2909..3547)
                     /locus_tag="VF958_07795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017523297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HEX9149044.1"
                     /translation="MKGRIRSGVFLGVTALAAAVGCRQEMYDQPKYQPLQKSGFFADG
                     RASRPLVEGTVARGMLTSGTAAVPPGAAPMASTELVTTLPVPLTRELVARGHERFGIF
                     CTPCHDRTGSGTGMVVRRGYRQPPSLHIDRLRDAPIGHFYDVMTRGLGAMPDYAQQIP
                     PADRWAIAAYVKALQLSQRATVAELPVEDRARLEAGSRETGDGKRGTKEPGH"
     gene            complement(3544..4095)
                     /locus_tag="VF958_07800"
     CDS             complement(3544..4095)
                     /locus_tag="VF958_07800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016481618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3341 domain-containing protein"
                     /protein_id="HEX9149045.1"
                     /translation="MTPPAAIGPRMWGLLAEFETPDELLSAAARAREAGYRRMDAYTP
                     FPIHGLSDAMGFRPTRLPLIVLAGGLIGCATALGMMWYSATIHYPLNVGGRPFASWPA
                     FIPITFELTVLFASLSAVFGMLGMNGLPMPYHPVFNAPRFAFASRDRFFFCIEARDAL
                     YDPAKTRAFLSSLGAREVVEVEE"
     gene            complement(4092..5501)
                     /gene="nrfD"
                     /locus_tag="VF958_07805"
     CDS             complement(4092..5501)
                     /gene="nrfD"
                     /locus_tag="VF958_07805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020711418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NrfD/PsrC family molybdoenzyme membrane anchor
                     subunit"
                     /protein_id="HEX9149046.1"
                     /translation="MSDTARRGRAGPPVIEPGHTYASVTDKISSIVLARRTPLFWLIG
                     FFLAFCLTMALLFAIGRLFAKGIGIWGVNQPVGWGFAIINFVWWIGIGHAGTLISAIL
                     LLLRQEWRTSINRFAEAMTLFAVACAGLFPILHMGRPWLAYWLIPYPNTMALWPQWRS
                     PLVWDVFAVSTYATVSLLFWFVGMIPDLATLRDRARRKGFQIFYGMAAMGWRGSALHW
                     QRYEAAYLLLAGLATPLVISVHTVVSFDFTIGIVPGWHSTIFPPYFVAGAIYAGFAMV
                     LLLAIPLRVVYGLEDFITMRHLQNMAKVMLATGLIVGYGYVIEAFMGWYSASPYETYM
                     IANRMRGPYAPVYWALMLCNIVAPQFIWWKRVRSSPAALFLIAVIVSIGMWLERFIIV
                     VTSLHRDFLPSSWGMYSPTIWDWTTFFGTIGLFLALMFLFIRFLPMISIFEMRTLVPQ
                     AAPLGMQPVPEESVAGEEG"
     gene            complement(5498..>8327)
                     /locus_tag="VF958_07810"
     CDS             complement(5498..>8327)
                     /locus_tag="VF958_07810"
                     /inference="COORDINATES: protein motif:HMM:NF024247.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HEX9149047.1"
                     /translation="VARRTFLKLMGASLALAGVTACTRQPAEKIVPYVRQPEEIIPGK
                     PLFYATAMSMGGLATGLLIESHMGRPTKVEGNSLHPASLGSTDAFAQAAVYSLYDPDR
                     SQTLLALGEIRPWPSFLGEMGIVLAEERPRRGAGLRILTETVSSPTLARQLAELLAKL
                     PAARWHQWEPASRDNARNGARMAFGAPVNAIYRLADADVILSLDADFLASDRSHVRLA
                     KEFAARRREGAEGRSMSRLYVVESTMTATGARADHRRPLQAAEVEAFARSLASRLGLG
                     APSGTAESGGRFTTDPLVRALAADLTRHRGRSVVIAGDAQPPAVHAIAHAMNGVLANA
                     GRTVVYTDPIEANPEDQLESIRALAADMQSGAISTLLVLGGNPVFTAPADLRFRDAIR
                     KVARRVHLSLESDETSAECQWNVPEAHFLEAWSDARAFDGTASIVQPLIAPLYGGRSA
                     HEVLAALSEQPEKSAYELVRETWSAHLPGNFEESWRRALHDGVIAGSAFPQRIPTGAA
                     VVPPGPAAGDSDRALEITFRPDPTIHDGRFANNGWLQELPKPVTKVTWDNVASVSPAT
                     AVRLGVKTEDVVELSLSGQSVRAPIFVVPGHPDDSIGVTLGYGRTMAGRLGSKTGFDA
                     YAIRSSRALWHANALEVRKTGDTWRIAVTQEHHSMEGRHLARAVTAEELARNPGAVQE
                     MGEPPSPGQTLYPPHPSHDYAWGMAIDLSSCVGCNACVVACTAENNVPVVGKDQVLRG
                     RELQWLRIDHYAEGDPSNPKHSFQPVACMHCETAPCELVCPTGATVHSAEGLNDMVYN
                     RCVGTRYCANNCPYKVRRFNFYHWSTQFRAPSMRMLANPDVTVRWRGVMEKCTYCVQR
                     INEGKIKAELENRRVRDGDIVTACQQACPAEAIVFGDLADPQSRVSQRRRSPLSYGLL
                     ADLGTRPRTAYLAKVDSPNVELAEPGRG"
BASE COUNT         1464 a         2729 c         2939 g         1195 t
ORIGIN      
        1 gtcccgcttg atgcggaacg cgggcacgta gaagctgtgg atggtgtcct ccgaggtcat
       61 ggtgagccgc acgggacggc cgaccgggac gtggagctcg ttgatctccc gctgaccgtc
      121 ggcgtgctgg accttccaca tccactgctt tccgacgacg gagacctcga gagcgtcggg
      181 tggcggccgg ttgatcgtga agaagactct cgccccccag aagaagaagg acagagcgat
      241 cacgagaggg atgaccgacc aggcgatctc gagcttgaga gacccttcga ccccgcgcgg
      301 cagttcggac tcggagcggc gacggtactt gatcgcgaag tacaggatgg aggccgcgat
      361 caggagggtg aagacgacgc tgacaatgat caggtagaag tagagcgcat cgacgcgtcc
      421 ggcgacggtc gaggccgcat tcggaaagag ggggaacgtc tctttcaacg ggcctccacc
      481 gcacgattcg ccgcgagccg acgctcgcgc cgccacatca ggaaaagaaa agtggacagg
      541 atgaggacga acgcgacgcc gccgaggcgc accatgttca tcacgaccgc cccgtatcgc
      601 ccggtcgccg ggtcgtagtg gtagcagtag aggaggatct tgtcgaccgg ggtccctatc
      661 ttgccctggg acgcctcgac gatcgcgagg cgcagatcgc gcggcgcgta ctcgattccg
      721 tagaagtagc gcgccagccg cccgtccggg gtcgcgacgt agatggcgct cgcgtgcgcg
      781 aactgtccga tcttttcgtc ccaggcgtat cggaacccca cggcgttcgt cagggcggca
      841 atcgacggcg gctcgccggt gaggaagtgc cagccggccg ccccgcccgt ccgcccgtat
      901 cgcgccacgt acggctcctt cttcgcgcgc gcgtcggacg gatcgtcccg cggatcgaag
      961 ctgaccgtga cgacctcgta ctcgcgtccg acgtcgaacg agagaacgcc catcgccgac
     1021 acgaggccgt tcagaacctg ggtgcacagc atcggacagt cgtaataggc gaggacgagg
     1081 acgaccggcc gcttcccgaa gtagtcgcgc aggcggacgg tgcgacccgt ttcgtccttg
     1141 aactcgaggt cgagcgggag tggtcggtcg aggtgttgct cgatgtcgac gccccggagg
     1201 atcggcggcc gcccgtcggg cgcgggcggc gcgggctgcg cgggcagtgc ggtcgagatc
     1261 gcgagaacgg cgagaactac ggcaacggtt ctcatcgttt cccgtttccc gtttcgcgtt
     1321 ttccgggtcc cggagtcggt gccggggtca tcacgggcat catgggtttc gcggggggca
     1381 acccgcgctc gaccaggatc tccatcgcgc ggtcgatcgg aatccgagcg atccccccgt
     1441 tccggtccac ccagccatag gactcgagaa tggcatcctc gcgggcctgg gccgcgacgc
     1501 gcggcgcgag cgggtccggt tcgaggcgcg gttcgcgcgg ggtgcgacgc aggctcgcgg
     1561 cgaccagcgg cggcaccggc tgatcgcgct tcgctgcacc ggcctgaaac gaccgcaaga
     1621 gtccccacat cgcgaccagc acaagagcgg cgacgatcgc gagcccaaca ccgaacttga
     1681 cgatcgcgga aatcgagacg tccgacgtct cgtgtcccgc ctcgggcgtg ggagaggccg
     1741 gatcagtgtg tgccatgcgc gaccgcctgc agcatttcgg gatcgttcat cggcaggagc
     1801 ggccgcttgc cgagctgcca gagaaacgtg gcgagccaga gtccgcccac gccgaccggg
     1861 gcaaggacgt ccatccagtg aagtcggaaa tgccccccgg cgaacgcggg ctgcgtgagc
     1921 cagaagacct cgatcaagcg catgaagaga acgaggccgg cgacggccgc gaggacacgc
     1981 ggattgcggt tcgcggacgc gggcaggagg aggaggaagg gaagcaggaa atggaagacg
     2041 atgagcgcga tcccgaccca gccccacccg ccctgcagcc ggcgcaggta ccacgggatc
     2101 tcctccggaa ggttacccgc ccagatgatc aggtactgcg aaaacgagac gtacgcccag
     2161 aacattacga aggcgagcat gagtttgccg aggtcgtggt agtggcgctg cccgacgtag
     2221 ccggcgagcg gcggggtgcc cgcaagcagc acgagcacga cgacgatgaa cgcgaacgcg
     2281 gtgagtccct gtcccgcgat gaacaggact ccgtacatgg tcgagaacca gtgcggctcg
     2341 agcgacatcg cccagtcgat cgatgcgaac gtcaccgtca gcccgtacgc gacgaggccg
     2401 gccgaggaga ggagctggag tcgccgcgcg atcggccgcg cgcccgtcgc atcctgctcg
     2461 agagaccagc ggttcaggaa atacgccagc gcgatccacg cggcgaagta gaaaagcgcg
     2521 cggcccgtga aaaaaggaag gttcaggtaa aggctcttgt ggcgcagcac cggatcgcgc
     2581 gcgaccgtcg cggggtccgc ccactcgtag atcctgcgcg ctccgaagac gatcggaaga
     2641 aaaagcagaa ggaggagcgg cagcgtccgg gttccggact cgagcggccg ccggatcgcc
     2701 acaccccacg cgccgccagt gacgtggttg agcatcgcga ccgccatgca tccgagcgcc
     2761 aggccggtcc agaagaggaa cgcgaacaga taggagcgga agaactgcgc cggcgcgaag
     2821 aagcccccga cggcgcagat cgccagcgcg gccgccccga cgaggaaggc gggtcgctgg
     2881 aaccgctcga atccgtgcac gtgcgccatc agtgcccggg ttccttcgtt ccccgtttcc
     2941 cgtctcccgt ttcccgactc ccggcttcca ggcgcgcccg atcctcgacc ggcagctcgg
     3001 ccacggtcgc gcgctgtgac agctgaaggg ccttgacgta ggcggcgatc gcccagcggt
     3061 cggccggcgg gatctgctgc gcgtagtcgg gcatcgcgcc gagcccgcgc gtcatcacgt
     3121 cgtagaagtg gccgatcggg gcgtcccgaa ggcgatcgat gtggagggaa gggggctgcc
     3181 ggtatccccg ccgcacgacc attcccgtgc cgctcccggt gcggtcgtgg cagggcgtgc
     3241 agaagatccc gaagcgctcg tggccgcggg cgacgagctc gcgggtcagc ggcaccggga
     3301 gcgtcgtcac gagctcggtg gacgccatgg gcgcggcccc cggcggcacg gcagcggtgc
     3361 ccgaggtgag catgccgcgc gcgacggtgc cttccacgag cggccgcgac gcgcggccgt
     3421 cggcaaagaa tccgctcttc tgaagcggtt ggtacttcgg ctggtcgtac atctcctgcc
     3481 ggcacccgac cgcggccgcg agagcagtca ccccaagaaa cacgccggag cggatgcggc
     3541 ctttcattcc tcgacctcga ccacttcacg ggcgcctaag ctcgacagga acgcccgggt
     3601 cttcgcgggg tcgtagagcg cgtcccgggc ttcgatgcag aaaaagaacc ggtcgcgcga
     3661 cgcgaacgcg aacctcgggg cgttgaacac ggggtggtag ggcatcggca gtccgttcat
     3721 cccgagcatc ccgaagaccg cggagagcga cgcgaagagc accgtcagct cgaacgtgat
     3781 cgggatgaac gcgggccagc tcgcgaacgg acgcccgccg acgttcaggg ggtagtggat
     3841 cgtcgccgaa taccacatca tcccgagcgc ggtggcgcag ccgatgaggc caccggcgag
     3901 cacgatgagc ggcagcctgg tcggacgaaa gcccatcgcg tcggagaggc cgtggatcgg
     3961 aaacggcgtg tacgcgtcca tccggcggta tcccgcctcc cgcgcgcgag ccgcggccga
     4021 caagagctcg tcgggagtct cgaactccgc gagcagcccc cacatgcgcg gcccgatcgc
     4081 cgccgggggc gtcacccttc ctccccggcg accgactcct cggggaccgg ctgcatcccg
     4141 agcggggcgg cctgcggaac gagggtgcgc atctcgaaaa tggagatcat cggcaggaac
     4201 cggatgaaga ggaacatgag cgcgaggaag agcccgatcg ttccgaagaa cgtcgtccag
     4261 tcccagatgg tcggcgaata catcccccac gacgacggca ggaagtcgcg atggaggctc
     4321 gtcacgacga tgatgaaccg ctcgagccac atgccgatcg agacgatgac ggcgatcagg
     4381 aacaaggcgg ccgggctcga tcgcacgcgc ttccaccaga tgaactgcgg cgcgacgatg
     4441 ttgcagagca tcagcgccca gtagacgggc gcatacggcc cccgcatccg gttcgcgatc
     4501 atgtaggtct cgtacgggct cgcgctgtac caccccatga acgcttcgat cacgtagccg
     4561 tagcccacga tgaggccggt ggccagcatc accttcgcca tgttctgcag gtgacgcatg
     4621 gtgatgaagt cttcgaggcc gtagacgacg cgcagcggta tggccaacag gaggaccatc
     4681 gcgaaacccg cgtagatcgc gcccgcgacg aagtaagggg ggaagatcgt ggaatgccag
     4741 ccgggaacga ttccgatcgt gaagtcgaag gacacgaccg tgtggaccga gatgaccagc
     4801 ggagtcgcga gcccggcgag aaggagatag gcggcctcgt accgctgcca gtggagcgcg
     4861 gacccgcgcc agcccatcgc ggccattccg tagaagatct gaaatccctt cctccgcgcc
     4921 cggtcgcgca acgtcgcgag gtcggggatc atcccgacga accagaagag gagtgaaacc
     4981 gtcgcgtagg tcgaaacggc gaagacgtcc cacacgagcg ggcttcgcca ctgcggccac
     5041 agagccatcg tgttcggata ggggatgagc cagtacgcga gccagggacg gcccatgtgc
     5101 aggatcggga acagaccggc gcaggccacg gcgaagaggg tcatcgcctc cgcgaagcgg
     5161 ttgatcgagg tgcgccactc ctgccgcagg agcagcagga tcgcggagat gagcgtgccg
     5221 gcgtggccga tcccgatcca ccagacgaag ttgatgatcg cgaagcccca gccgaccggc
     5281 tggttgacgc cccagatccc gatgcccttc gcgaagagcc ggccgatcgc gaacaggagc
     5341 gccatcgtca gacagaacgc gaggaaaaac ccgatcagcc agaagagcgg agtgcgccgc
     5401 gcgaggacga tcgaactgat cttgtcggtg acggacgcgt aggtgtgacc gggctcgatc
     5461 accggcggac ctgcgcggcc gcgccttgcg gtatcgctca tccgcgtccc ggctccgcca
     5521 gttcgacgtt gggactatcg accttcgcca ggtacgccgt cctcgggcgc gtgccgagat
     5581 ccgccagaag cccgtaggag agcggcgacc gcctgcgctg cgagacccgg ctctgcggat
     5641 cggcgaggtc gccgaacacg atcgcctcgg cggggcacgc ctgctggcag gccgtgacga
     5701 tgtcgccgtc gcggacgcgg cggttctcga gctcggcctt gatcttccct tcgttgatgc
     5761 gctgcacgca gtacgtgcac ttctccatga cgccgcgcca gcgcacggtc acgtcgggat
     5821 tggcgagcat ccgcatgctc ggtgcgcgga actgcgtgga ccagtggtag aagttgaacc
     5881 gccggacctt gtaagggcag ttgttcgcgc agtagcgcgt cccgacgcag cggttgtaga
     5941 ccatgtcgtt caggccctcg gcgctgtgaa ccgtcgcgcc ggtcgggcag acgagctcgc
     6001 agggcgcggt ctcgcagtgc atgcaggcga cgggctggaa cgaatgtttc gggtttgagg
     6061 ggtccccctc ggcatagtgg tcgatgcgga gccactgcaa ctcccggccg cgcagcacct
     6121 ggtccttccc gacgaccggg acgttgttct cggcggtgca cgcgacgacg cacgcgttgc
     6181 agccgacgca gctcgaaagg tcgatggcca tgccccacgc gtagtcgtgc gaggggtgtg
     6241 gcgggtagag cgtctggccg ggggagggcg gttcccccat ctcctggacg gcgccgggat
     6301 tgcgggcgag ctcctccgcg gtgaccgcgc gcgcgaggtg ccttccctcc atcgagtggt
     6361 gctcctgggt caccgcgatc cgccacgtgt cgccggtctt tcgaacttcg agcgcgttcg
     6421 cgtgccaaag cgcgcgggag gagcggatcg cgtaagcgtc gaagccggtc ttcgagccga
     6481 gccggcctgc catcgtgcgc ccgtagccga gcgtgacccc gatcgagtcg tccggatggc
     6541 cgggcacgac gaagatcggc gcccgcacgc tctggcccga cagggaaagc tcgacgacgt
     6601 cttcggtctt cacgcccagg cggacggcgg tcgccggcga gacggaagcg acgttgtccc
     6661 aggtcacctt cgtcacgggc ttgggcagct cctgcagcca gccgttgttc gcgaagcggc
     6721 cgtcgtggat cgtgggatcg ggccggaagg tgatctcgag ggctcggtcg gaatctccgg
     6781 ccgccggtcc cggagggacc accgcggccc ccgtcggtat gcgctgcggg aatgcgctgc
     6841 ccgcgatcac tccgtcgtgg agggcgcggc gccaggattc ctcgaaattt ccggggaggt
     6901 gagcgctcca ggtctctcga acgagctcgt acgcggactt ctccggctgt tccgacagcg
     6961 ccgcgaggac ctcgtgcgcc gatcggccac cgtagagcgg cgcgatcagg ggctggacga
     7021 tcgaagcggt gccgtcgaac gcccgcgcgt cgctccaggc ctcgaggaag tgcgcctcgg
     7081 ggacgttcca ctgacactcg gcggaggtct cgtcgctctc gagagagagg tggacgcggc
     7141 gagcgacctt gcggatggca tcgcggaatc tcaggtccgc cggcgcggtg aacacggggt
     7201 ttccgccgag gacgagcagc gtcgagatgg cgccgctttg catgtcggcg gcgagtgcgc
     7261 gaatcgactc gagctggtcc tcggggttcg cctcgatcgg gtccgtgtag acgaccgtcc
     7321 gccccgcgtt cgcgaggacc ccgttcatcg cgtgcgcgat cgcgtggacc gcaggcggct
     7381 gcgcatctcc ggcgatgacg acgctccggc cgcggtgacg ggtgagatcg gcggcgagcg
     7441 cgcgaacgag cggatcggtg gtgaagcgtc cgcccgactc cgccgtgccc gacggagcgc
     7501 cgaggccgag ccggctcgcc agcgagcgcg cgaacgcctc gacctccgcc gcctggagcg
     7561 gcctgcggtg gtccgcgcgc gcgccggtgg ccgtcatcgt gctctcgacg acgtagagcc
     7621 ggctcatcga acgcccctcg gctccctccc gtcgccgggc cgcgaattcc tttgcgagac
     7681 gcacgtgcga ccggtcggag gcgaggaagt ccgcgtcgag cgaaaggatg acgtccgcgt
     7741 ccgcgagacg gtagatcgcg ttgacgggag ctccgaacgc catcctcgcg ccgttcctcg
     7801 cgttgtcgcg cgatgcgggc tcccactggt gccagcgcgc ggcgggaagc ttggccagta
     7861 gctcggccag ctgccgcgcg agggtcggcg aggagaccgt ctccgtcaga atccgcaggc
     7921 ccgctccgcg ccgcggtcgc tcctccgcga gaacgatccc catctcgccg aggaagctcg
     7981 gccacggacg gatctccccc agcgcaagga gcgtctggga cctgtctgga tcgtagagcg
     8041 aataaaccgc ggcctgcgcg aatgcatccg tactgccgag gctcgccgga tgcagcgagt
     8101 tgccctcgac cttcgtcggc cgccccatgt ggctctcgat gaggaggccg gtcgcgagcc
     8161 cgcccatgct catcgcggtc gcgtagaaga gcggcttgcc cgggatgatc tcttcgggct
     8221 ggcggacgta cgggacgatt ttctcggccg gctgccgcgt gcaggcggtg acgcccgcga
     8281 gcgccagcga tgcgcccatc agcttgagga acgtgcgccg cgccacc
//
LOCUS       DASWAV010000371         1527 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U2665
            k141_699869, whole genome shotgun sequence.
ACCESSION   DASWAV010000371 DASWAV010000000
VERSION     DASWAV010000371.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960316
            Sequence Read Archive: SRR10260007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 1527)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1527)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:47:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,929
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1527
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_699869"
                     /isolate="SMAG_U2665"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.36444 N 132.31889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..671
                     /locus_tag="VF958_07815"
     CDS             <1..671
                     /locus_tag="VF958_07815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010943019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="anthranilate synthase component I family
                     protein"
                     /protein_id="HEX9149048.1"
                     /translation="AVFGASPEMLVRCRGRLAETRPIAGTIRRGATPEEDANLAARML
                     ADPKERAEHVMLVDLGRNDLGRVCELGTVEVTRYAEVERYSHVQHLVSDVRGTLAPGK
                     TSTDALASCFPAGTLTGAPKIRAMELIDELEGFRRGIYGGAVGYLDHGGNLDMAIAIR
                     TAVVEKQVCRIQAGAGIVADSVAENEYREAESKAEALFRAVESAREKSGPIPDFRFPI
                     PEAR"
     gene            668..1273
                     /locus_tag="VF958_07820"
     CDS             668..1273
                     /locus_tag="VF958_07820"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011389648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004049 - anthranilate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046820 - 4-amino-4-deoxychorismate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate/anthranilate synthase
                     component II"
                     /protein_id="HEX9149049.1"
                     /translation="MIFFVDNYDSFTWNLVQAIGKVDADVEVARNDRFDPESVVAKRP
                     SAIVVSPGPGRPERAGRSLETIALAEREGIPLLGVCLGHQAIAALHGGVVERAPAPRH
                     GKSSRVRHGGGELFAGIPSPFDAGRYHSLAVREEGLPEDLEVTAVSEDGVVMAIAHRS
                     KPVYGVQFHPESVLTPHGETLLGNFLRLARARAGDAPGGVP"
     gene            1270..>1527
                     /locus_tag="VF958_07825"
     CDS             1270..>1527
                     /locus_tag="VF958_07825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007427437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate phosphoribosyltransferase"
                     /protein_id="HEX9149050.1"
                     /translation="MTPHVAEALKVVAARRPLPRDLAERVFGDLMDGQATEAQKGAIL
                     IGIATRGETADEIAGAVAALRARMRRVTTSRAPLLDTCGPGG"
BASE COUNT          242 a          502 c          543 g          240 t
ORIGIN      
        1 cggcggtttt cggggcctcg cccgagatgc tcgtgcgttg ccgcgggcgt ctcgccgaga
       61 cgcggccgat cgccggtacg attcgccgcg gggccactcc cgaggaggat gcgaacctgg
      121 ccgctcggat gctcgcagat cctaaggaga gagcggaaca cgtcatgctc gtcgatctgg
      181 ggcgcaacga tctcggtcgg gtgtgcgaac tcggaaccgt cgaagtcacc cgttacgccg
      241 aggtcgagcg ctactcgcac gtgcagcatc tcgtgtccga cgtgcgcggc acgctggcgc
      301 ccggcaagac atccaccgac gcgctcgcat cctgtttccc ggcgggaacc ctgaccggtg
      361 ccccgaagat tcgcgcgatg gagctgatcg acgagctcga aggttttcgt cggggcatct
      421 acggcggagc ggtcggctac ctggaccacg gaggcaacct cgacatggcc attgcaattc
      481 ggacggcagt ggtcgagaag caggtctgcc ggatccaggc gggcgctggc atcgtcgcgg
      541 actcggtcgc ggagaacgaa taccgcgagg ccgagtcgaa agccgaggct ctcttccggg
      601 ccgtggagag tgcccgcgag aaatccggcc cgattcccga tttccgattc ccgattcccg
      661 aggcccgatg atcttcttcg tcgacaacta cgactccttc acctggaacc tcgtgcaggc
      721 gatcgggaag gtggacgcgg acgtcgaagt cgcacgcaac gatcgcttcg acccggagtc
      781 ggtcgttgcg aagcgtccct cggcgatcgt cgtgtcgccc ggccctggcc gtccggagcg
      841 tgccgggagg tcgctcgaaa cgatcgcgct cgccgaacgc gagggcatcc cgctgctcgg
      901 agtctgcctg gggcatcagg cgatcgccgc gctgcacggc ggcgtcgtcg agcgcgcgcc
      961 tgcgccgcgg cacggcaaga gctcacgcgt ccgtcacgga ggaggtgagc tgttcgccgg
     1021 aatcccgagc ccgttcgacg ccgggcggta ccattccctc gccgtgcgcg aagagggatt
     1081 gcccgaggat ctcgaggtga cggcggtgag cgaggacggg gtcgtcatgg cgatcgctca
     1141 ccgttcgaag ccggtctatg gagtgcagtt tcaccccgag tctgtcctga cgcctcacgg
     1201 ggagacgctg ctcggcaatt tcctccggct cgcgcgggct cgcgcaggcg atgcgcccgg
     1261 gggagtgccg tgacgcccca cgttgccgaa gcgctgaagg tcgttgccgc ccgaagaccg
     1321 ctgcctcgcg acctggcgga gcgggtcttc ggcgatctca tggacggaca ggcgacggag
     1381 gcacagaagg gcgcgatcct gatcggaatc gccacgcgcg gcgagacggc ggacgaaatt
     1441 gccggcgcgg tggcggcgct gcgggcacga atgcgccggg tcacgacctc ccgtgcgccg
     1501 ctcctcgaca cctgcggacc cggaggg
//
LOCUS       DASWAV010000372         2228 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U2665
            k141_377749, whole genome shotgun sequence.
ACCESSION   DASWAV010000372 DASWAV010000000
VERSION     DASWAV010000372.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960316
            Sequence Read Archive: SRR10260007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2228)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2228)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:47:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,929
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2228
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_377749"
                     /isolate="SMAG_U2665"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.36444 N 132.31889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1141
                     /locus_tag="VF958_07830"
     CDS             <1..1141
                     /locus_tag="VF958_07830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9149051.1"
                     /translation="NDYISTGSNRNSYKNAWQPRIGLSYDVLGNGNTVFTGAWGRYWD
                     HVNLQDIYVEQQRQGWKRFSFCFTDPRTTASPTCPNPITWNDNYLSRAGLEGLIASGI
                     AGGPEVFLLQNSTKPPRSDQWNVGVHQRLGSYLFGLSYNNVRGYNGLIWFPATPLDIG
                     PDRPDRGGHQFRATGFGTILYSSYSRRTWYDGWFLTAERPFTTASNWGFTFTYTYAHA
                     KQLGKENKIEDTEFGFDFLNPQNLRKVRGDNDERHHIAASAIYGLPWDIRFSAFLTLG
                     SGVPFTIFDFSREAASIKWNGGNPPRRRNIFGYWAYESLDLRLSKDFMVTRGVRLGVD
                     AQVFNVTNFKNYCNFIDFYLDPNLGKPRDCQYNTRRAQVGVNVGF"
     gene            1162..>2228
                     /locus_tag="VF958_07835"
     CDS             1162..>2228
                     /locus_tag="VF958_07835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010543036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucoamylase family protein"
                     /protein_id="HEX9149052.1"
                     /translation="MPRRDVDPERRRSRRGTAGAVAALFFVLVACRPATRPVAVARQE
                     AGRYEELLDLVERRTFDFFWDLANPDNGLVPDRWPTPSFSSVAAIGFGLTAYPIGVER
                     GWVTREDARRRVLTTLRFLSSAPQGPSSRGVTGYKGFFYHFLDVKTGERFETVELSTI
                     DTTLLVAGVLLCQSYFDGESSEEAEIRSLAESLYGRIEWDWAQPRPPLVNHGWKPETG
                     FLPHDWGGGGYDESMILYVLALGSPTHPIAPEAWERITANFEWTRFYGQEYIPFNALF
                     GYQYSHVWIDFREIQDRFTQARGIDYFENSRRAAYAHRAYAIANPGGWRDFGETIWGL
                     TACDGPADVTATIEGRVRTFHT"
BASE COUNT          400 a          737 c          689 g          402 t
ORIGIN      
        1 gaacgactac atcagcacgg ggtcgaaccg aaattcctac aagaacgcct ggcagccgcg
       61 catcggtttg agctacgacg tgttgggaaa cggaaacacg gtcttcacgg gagcctgggg
      121 ccgctactgg gaccacgtga accttcagga catctacgtc gagcagcagc ggcaaggctg
      181 gaagcgcttc tcgttctgtt tcaccgatcc gcgcacgacg gcgtcgccga cctgcccgaa
      241 ccccatcacc tggaacgaca actacctctc ccgcgcaggg ctcgaagggc tgatcgcctc
      301 gggaatcgcc ggcgggccgg aggtctttct cctacagaat tccacgaagc ctccgcgcag
      361 cgatcagtgg aacgtcgggg tccaccagag actcgggtcc tatctcttcg gcctctccta
      421 caacaacgtc cgcggctata acggcctcat ctggtttccc gccacgccac tggatatcgg
      481 gcccgacagg ccggaccgag gcgggcacca attccgggcg accggattcg gcacgatcct
      541 ctactcgagc tacagccgtc ggacgtggta cgacgggtgg ttcctcacgg ccgagcggcc
      601 cttcaccacc gcgtccaact ggggctttac gttcacgtac acgtatgccc acgcgaagca
      661 gctcggcaag gagaacaaga tcgaagacac cgagttcgga ttcgattttc tcaaccccca
      721 gaacctccga aaagtgcgcg gcgacaacga cgaacgccac cacatcgcgg cttcggcaat
      781 ctatggcctg ccgtgggaca tccggttctc cgcgttcctg acgctggggt ccggcgtacc
      841 gttcacgatc ttcgactttt cgcgggaagc cgcgtcgatc aagtggaacg gcgggaaccc
      901 ccctcgccgg cggaacatct tcggttactg ggcctacgag agtctcgatc tgcgcctctc
      961 gaaggatttc atggtgaccc gcggagtacg gctcggcgtc gacgcgcaag tgttcaacgt
     1021 gacgaacttc aagaactact gcaacttcat cgacttctac ctggacccga atctcggcaa
     1081 gccgagagat tgccagtaca acacgcggcg cgcccaggtc ggcgtgaacg tgggattctg
     1141 aggcacggct gaccggttcc cgtgccgcgg cgcgacgttg atcccgaaag acgccgttcg
     1201 cgccgcggca cggccggagc cgtcgccgcc cttttcttcg tcctcgtcgc gtgtcgtccg
     1261 gcgactcggc cggtcgccgt cgctcgacag gaggccgggc gatacgagga gctcctcgac
     1321 ctcgtcgagc gtcggacctt cgatttcttc tgggatctcg ccaatccgga caatggcttg
     1381 gtgccggacc gctggcccac gccttcgttc tcgagcgtcg ccgcgatcgg cttcggtctg
     1441 accgcgtatc cgatcggggt cgagcgcgga tgggtgaccc gcgaggacgc ccggagacgc
     1501 gtgctgacga cgctccgatt cctttcgtcg gctccgcagg gaccgtcgtc gcgaggcgtc
     1561 accggctaca aggggttctt ctaccacttc ctcgacgtga agaccggcga gcgcttcgag
     1621 acggtcgagc tctcgacgat cgacacgacg cttctcgtcg ccggcgttct cctgtgccag
     1681 tcgtacttcg acggtgagag ctcggaggag gccgagatcc ggtcgctcgc cgaatcgctg
     1741 tacggacgaa tcgagtggga ttgggcgcag cctcgacccc ctctggtcaa tcacggttgg
     1801 aagcccgaga caggttttct cccgcacgac tgggggggcg gcggttacga cgagtcgatg
     1861 atcctctacg tcctcgcgct cggctccccg acgcacccga tcgcgccgga agcgtgggag
     1921 cggatcaccg cgaacttcga atggacgcgc ttctacgggc aggagtacat cccgttcaac
     1981 gcgctcttcg gctaccagta ctcgcacgtc tggatcgact tccgggaaat ccaggatcga
     2041 ttcacccagg cgcgcgggat cgactacttc gagaattcgc ggcgggcggc gtacgctcat
     2101 cgggcttacg cgatcgcgaa ccccggcggt tggcgcgatt tcggagaaac gatctggggc
     2161 ctgaccgcct gcgacgggcc ggcggacgtc accgcgacga tcgagggacg ggtccggacg
     2221 ttccatac
//
LOCUS       DASWAV010000373         2321 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U2665
            k141_99791, whole genome shotgun sequence.
ACCESSION   DASWAV010000373 DASWAV010000000
VERSION     DASWAV010000373.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960316
            Sequence Read Archive: SRR10260007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2321)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2321)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:47:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,929
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2321
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_99791"
                     /isolate="SMAG_U2665"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.36444 N 132.31889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..911
                     /gene="hflX"
                     /locus_tag="VF958_07840"
     CDS             <1..911
                     /gene="hflX"
                     /locus_tag="VF958_07840"
                     /inference="COORDINATES: protein motif:HMM:TIGR03156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HEX9149053.1"
                     /translation="EARTQVSLAQLQYLLPRLARRYGHLSRLAGGIGGRGAGEQKLEL
                     DRRKIRDRIARLRHDLEKIETSRKVRRRSRAGRFQVAIAGYTNAGKTTIFNRLTREAA
                     YAADRLFATLDSRTAHGASSKISQVVFVDTVGFVRKLPPSLVASFRSTLSEIRDADLV
                     LHVLDASSPRVDEERLVALEVLEELGVDPARILPVWNKTDLPGAPTPRGEIGVSARTG
                     AGFEVLERAILQRARPEPDHFRLRIPYANGRAISAARASFRVMEEHDLGDALALAVAG
                     ERKNLGPLARYEEALEETAALSRRAR"
     gene            1147..1386
                     /locus_tag="VF958_07845"
     CDS             1147..1386
                     /locus_tag="VF958_07845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9149054.1"
                     /translation="MKRAIRLVLFVLAAAVTMACSRGHRTEAATERGTPDLPPPTPDT
                     TPIPALRTPAGLFLGVVGTPAAASAAPAATPTPVP"
     gene            1598..2188
                     /locus_tag="VF958_07850"
     CDS             1598..2188
                     /locus_tag="VF958_07850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008928872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008199 - ferric iron binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HEX9149055.1"
                     /translation="MKSKPRKPGKSGAQGEFLTDVKELRRRARRHIEDGAVTSSYGAD
                     RDTVVRIMNDALATEIVCVLRYKRHYFMAEGIHATAVAQEFLQHATEEQGHADRIAER
                     ITQIGGAPNFNPEGLTSRSHSEYVEGDSLVDMIREDLVAERIAIESYGEIVRFLGDRD
                     ITSRRMMEEILANEEEHADDLQSLLVTLDPTKTGSK"
BASE COUNT          415 a          806 c          759 g          341 t
ORIGIN      
        1 gggaggcgcg aacgcaggtc agcctcgcac agctccaata tctcctgccg agactggcca
       61 ggaggtacgg ccacctctcg cggctcgcgg gcggaatcgg cgggcgcggc gcaggcgaac
      121 aaaagctcga gctcgaccgg cgcaagatcc gcgaccgcat cgcgcggctg cgacacgacc
      181 tcgagaagat cgagacgtcc cgcaaggtgc gccgccgctc ccgcgccggg cgcttccagg
      241 tcgcgatcgc cggctacacc aatgccggaa agacgacgat cttcaaccgg ctgacgcgcg
      301 aggcggcgta cgcggccgac cgtctgttcg cgacactcga ttctcggact gcgcacggcg
      361 cctcctcgaa aatctcgcag gtcgttttcg tcgacacggt cgggttcgtg cgcaagctgc
      421 cgccgtcgct cgtcgcgtca tttcgatcga cgctgtcgga gattcgcgac gccgacctcg
      481 tgctgcacgt gctcgacgcc tcgtctccgc gcgtggacga ggagcgtctc gtcgccctcg
      541 aagtgttgga agagctcgga gtcgatccgg cgcgaatcct tcccgtctgg aacaagaccg
      601 acctccccgg agccccgacc ccgcgagggg agatcggggt gtccgcccgg accggcgccg
      661 gcttcgaagt gctcgagcgg gcaatcctcc agcgggcgcg gccagagccg gatcacttcc
      721 gcctgcgaat cccgtacgcg aacgggcgtg ccatctcggc tgcccgggcc tccttccggg
      781 tcatggaaga gcacgacctg ggcgacgccc tcgccctggc cgtcgccggg gagcggaaga
      841 acctgggccc cctcgcgcgc tatgaagagg ccctcgaaga gacggcggcg ctctcccgga
      901 gggcccgctg acgtgaggac cgaaaaacaa aggggggaac ccttcggctc ccccccatgc
      961 cggaactccc atgccggcac tcttcttggc ttctgcataa ctcaaggccc gtaccagcat
     1021 ccggaggaga ctttcgcttg gcgctgcaaa gagttgcgcg aaatctcatc caccggtgag
     1081 tcaaactcgg tcagcgcgcc gcggccgaaa atgacaaaat ctgtcgacta caatcgtcac
     1141 ctccaaatga aacgggcgat ccgtctcgtc ctcttcgtgc tcgcggcggc cgtgacgatg
     1201 gcctgttccc gcggccaccg caccgaggcg gcgacggagc gcgggacgcc cgatctcccg
     1261 cctccgactc ccgacacgac accgatcccc gcgttgcgca ccccggccgg gctctttctc
     1321 ggggtcgtgg ggactccggc ggccgcctct gccgcgcctg ccgcgacgcc aacgccggtc
     1381 ccatgagtga gccgctcgac gaggggccgc ggacggcttc tgcccgcggc tcaccggtcg
     1441 aaacgaaccg aacgtccggt cccgagcgct tctgcccgaa ctgcggcgcc gagctcatcg
     1501 agaggagctg caagcttctc tgcccgaacc gacagtgcgg gtacgcgaag tcgtgctcgg
     1561 acttccacta gagtcgggac gtttgcaggg aggtgtcatg aagtcgaaac ccaggaaacc
     1621 cgggaagagc ggcgcgcagg gcgaatttct gacggacgtc aaggagctgc gccggcgcgc
     1681 gaggcggcac atcgaggatg gcgcggtgac gtcgagttac ggggcggacc gcgacacagt
     1741 cgtccgcata atgaacgacg ccctcgccac cgagatcgtc tgcgttctgc gctacaagcg
     1801 ccactacttc atggcggagg ggatccacgc gacggcggtc gcgcaggagt ttctgcagca
     1861 cgccaccgaa gagcagggtc acgccgaccg gatcgccgag cggatcaccc agatcggcgg
     1921 cgctccgaac ttcaatcccg agggcctgac ctcccgcagc cactccgagt acgtcgaggg
     1981 tgacagcctc gtggacatga tccgcgagga cctcgtcgcc gagcgcatcg cgatcgagtc
     2041 ctacggggag atcgtgcgat tcctcggcga ccgggacatc acgtcgcgcc ggatgatgga
     2101 agagatcctc gccaacgagg aggagcacgc ggacgacctg cagagcctcc tcgtcacgtt
     2161 ggatccgacg aaaacgggct cgaagtgagc ggaaggcggg gccgctcgcg ggggtgatag
     2221 actcatctag cccgatccct ccgacggtct ctcctgccac ctcgaggtca gcgcccgtcg
     2281 ccggttccgg cgagaaagcg agtgaccatg acagcaaccc g
//
LOCUS       DASWAV010000374         3605 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U2665
            k141_377792, whole genome shotgun sequence.
ACCESSION   DASWAV010000374 DASWAV010000000
VERSION     DASWAV010000374.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960316
            Sequence Read Archive: SRR10260007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 3605)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3605)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:47:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,929
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3605
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_377792"
                     /isolate="SMAG_U2665"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.36444 N 132.31889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2127)
                     /locus_tag="VF958_07855"
     CDS             complement(<1..2127)
                     /locus_tag="VF958_07855"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX9149056.1"
                     /translation="MSKPTPSEDAPFAPNTPIPGVRSSLKTRLKVTAFVGFAIVAAWL
                     YYTVNQVLTLHDVTQDIQRTTDLRERVGDAKNALAEAEDSLDRYTQNGEGYDHSRHHA
                     SRTTLRAALGAIRRRVMTESSRGALEQAVAAEEIFSKAADRAIALWAPGNPTAAREHR
                     DNVVEPAAASLREILNDLESRFGRGESIAEGRLKGARDSATASLVILAALIVAGVLWL
                     LTDVDRRILSPAMSATRALGDLASDRTPPLLADASGDEIGELGRQFNRAAASYADRAR
                     ALAERDIQASVNAVLAAAATVNDLKGFGSKVLETVLEVSGAASAILYLPEPGGSFTPA
                     EAVGWTRPEEDAVGAATARRVAEEKRPLFLSVDAGTPTIDVFDGRILPRESAHIPLIY
                     FDHVVGVLALGAAQPFTIGARNVLSAIAPSLAVALANAAANERVAEQTRRLAEQNELL
                     EEQRSRIARTNLELQRASALKDRFLASVSHELRTPMTVILGFTAALLKGGQGNLNTQQ
                     RESLERVQRNAKLLLGLINDVLDISKIESGKVEIRRQTVFPASLARQIEADYGEAARS
                     KGLKFVAETATGLDSVTSDPARVLQIIANLVGNALKFTEQGSITVRFEPREEARWAIV
                     VTDTGIGIPEDEQGAIFDEFRQGEAPEHRGHGGTGLGLAIVKKLAGVLGGTVSLESSP
                     GKGSRFTVVLPRELPRETPVIAAVAEP"
     gene            2180..2833
                     /locus_tag="VF958_07860"
     CDS             2180..2833
                     /locus_tag="VF958_07860"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HEX9149057.1"
                     /translation="MRLSSTSARDSARRRFLRTFFHDLATPLSAVSLHLEGANRRLVR
                     GEDPAGSLDIARSELGRAFDLFERGRELLLSEPADVESFGFDEWVEATLARFAGDGVE
                     VHGATGGRISGDREKLSEALLSLLVNAIEHGSSPPVAVFRERSGDRIQVRIENRGVLP
                     AEDPEKLFAPRTAAAGKNWGMGLALARLYAADAGGDILLSQKGENVAAILTFPEEPA"
     gene            2830..>3605
                     /locus_tag="VF958_07865"
     CDS             2830..>3605
                     /locus_tag="VF958_07865"
                     /inference="COORDINATES: protein motif:HMM:NF012385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HEX9149058.1"
                     /translation="MKVLIVEDDLATRKGLEESVKDLGGETRSVGSAKEASHALEEFD
                     PRLLIVDIHLPDGDGIEVLRAARETDPDREGIVITGQGSIDNAIEALRAGAVDYLLKP
                     LRPAQLEVVFNRLADHRRLEFEVETLRAELQETGRLGDLVGRSPVMTEIFDVIRRVAK
                     SNAPVLITGASGTGKEVAARTIHRLSKRAAKPFVAFNCGAISPTLIESELFGHERGSF
                     TGADKRRVGYFEEAAGGTLFLDEITEMGSELQVKLLRVLE"
BASE COUNT          580 a         1147 c         1258 g          620 t
ORIGIN      
        1 gggctcggca acggcggcaa tcacgggcgt ctcgcgcgga agctcgcgcg gaaggacgac
       61 cgtgaaacgg gagcccttgc cgggggagct ctcgagggaa accgtccctc cgaggacgcc
      121 cgcgagcttc ttgacgatgg caagaccgag tccggtaccg ccgtggccgc ggtgctcggg
      181 tgcttcgccc tgacggaatt cgtcgaagat cgcgccctgc tcgtcttcgg gaatcccgat
      241 gccggtgtcc gtgaccacga tcgcccaacg ggcctcctcc cgcggctcga accgaacggt
      301 gatggagccc tgttccgtga acttgagggc gttgccgacg aggttggcga tgatctgaag
      361 gactcgggcg ggatcgctcg tcaccgaatc gagacctgtc gcggtctccg ccacgaactt
      421 gagccccttg ctgcgcgcgg cctcgccgta gtcggcctcg atctggcgcg cgagggaggc
      481 cgggaaaacc gtctgcctgc ggatctcgac cttgcccgac tcgatcttgg aaatgtcgag
      541 cacgtcgttg atgagaccga gaagcagctt cgcgtttcgc tgaactcgct cgaggctctc
      601 gcgctgctgg gtattcaggt ttccctgtcc gcctttcaac aacgccgccg tgaagccaag
      661 gatcaccgtc atgggcgtgc ggagctcgtg agagacggaa gcgaggaagc ggtccttcag
      721 agcggaggcg cgttgaagct cgagattcgt ccgggcgatg cggctgcgct gttcttcgag
      781 cagctcgttc tgttcggcga gtcgccgagt ctgctcggca acccgctcgt tcgcggcggc
      841 gttggcgagc gcgaccgcga gactgggtgc gatcgccgaa aggacgttgc gcgccccgat
      901 ggtgaacggc tgcgccgcgc cgagcgcgag cacaccgacg acgtggtcga agtagatgag
      961 cgggatgtgc gcgctctcgc ggggaaggat ccggccgtcg aagacgtcga tcgtcggcgt
     1021 ccccgcgtcg accgagagaa agagcggtcg cttctcctcg gccacgcgcc gggccgtcgc
     1081 cgcgccgaca gcgtcctcct ccggccgcgt ccagccgacc gcctccgccg gagtgaatga
     1141 accgcccggc tccgggagat acaggatcgc ggacgcggcg cccgaaacct cgagaaccgt
     1201 ctcgagcact ttggatccga agcccttcag gtcgttcacc gtcgccgctg cggcgagcac
     1261 cgcattgacg gatgcctgga tatcgcgttc ggcgagcgcg cgcgcccggt cggcgtacga
     1321 ggcggccgcg cggttgaact gccggccgag ctccccgatc tcgtcgcccg aggcgtcggc
     1381 gagcagcggc ggcgtgcggt cggatgcgag atccccgagc gcgcgagtcg ccgacatcgc
     1441 cggggagagg atccgccgat cgacgtcggt caggagccac agcactcccg cgacgatcag
     1501 ggccgcgagg atcacgagag aggcagtggc cgaatcgcgc gctcccttca gccgaccttc
     1561 ggcgatcgat tcgccgcgcc cgaagcggct ttcgaggtcg ttcaagatct cgcgcagaga
     1621 cgccgcggcg ggctcgacga cgttgtcgcg gtgctcacgc gcggccgtgg ggttcccggg
     1681 cgcccagagc gcgatcgcgc gatccgccgc cttcgagaag atctcttcgg cagccaccgc
     1741 ctgttcgagc gctccgcggg agctctccgt catgacgcgc cggcggatgg ctccgagcgc
     1801 ggcgcgaagc gtcgtccggc tcgcatgatg tcgcgaatgg tcgtatccct ctccgttctg
     1861 tgtgtaacgg tcgagagagt cctcggcttc ggcaagcgcg ttcttcgcgt cgccgacgcg
     1921 ttcacgcagg tccgtcgtgc gctggatgtc ctgggtgacg tcgtggagcg tcaagacctg
     1981 gttgaccgtg taatagagcc acgcggcgac gatcgcaaag ccgacgaacg ccgtgacctt
     2041 gagtcgggtc ttcaaagacg accggacgcc cggtatcggc gtgttcgggg cgaagggggc
     2101 gtcctcggaa ggagtcggtt ttgacatacg atgctgtagt tcccttggag ggcgtggttt
     2161 tagcacaaag gatgcccact tgaggctgtc ttcgaccagc gcacgagact cggcccggcg
     2221 acgattcctc cggacctttt ttcacgacct cgcgacgccg ctttcggcgg tgtcgcttca
     2281 tctggaagga gcgaaccggc ggctcgtccg cggtgaagat ccggccggat cgctcgacat
     2341 cgctcggtcc gaactcgggc gcgcgttcga ccttttcgag cgcgggcggg aactgctgct
     2401 ctccgagccg gccgatgtgg agagcttcgg gttcgacgaa tgggtcgagg cgacgctcgc
     2461 tcgcttcgcc ggggacggag tggaggtcca tggtgcgacc ggaggccgga tttccgggga
     2521 cagggaaaag ctctcggagg ctctcctgtc gcttctcgtg aacgccatcg agcacgggtc
     2581 ttctccccct gtggcggtat tccgagagcg gtccggagac cgcattcagg tgcgcatcga
     2641 gaaccggggt gtcctgccgg cggaggatcc cgagaagctc tttgccccgc gcacggcagc
     2701 cgccgggaag aattggggaa tggggctcgc cctcgctcgg ttgtacgcgg ccgacgccgg
     2761 tggtgacatt cttctttctc aaaagggcga gaacgtcgcc gcaatcctga cgtttcccga
     2821 ggagcccgct tgaaggttct gatcgtcgaa gacgacctgg ccacccgcaa gggactcgag
     2881 gaatcggtca aggacctcgg cggggagacg cgatccgtcg ggagtgcgaa ggaggcgtcg
     2941 cacgctctcg aggaattcga tccgcgcctc ctgatcgtcg acattcacct tcccgacggg
     3001 gacggcatcg aggtcctgcg cgcggcgcgc gagaccgatc cggaccgcga gggaatcgtg
     3061 atcaccggac agggttcgat cgacaacgca atcgaggcgt tgcgcgcggg agcggtcgac
     3121 tacctgctga agccgttgcg gccggcgcag ctcgaggtcg tgttcaaccg actggcggat
     3181 caccgacggc tcgagttcga agtcgagacg ctgcgtgcgg agctgcagga gaccgggcgt
     3241 ctcggcgacc tggtcgggcg ttcgccggtc atgacggaaa tcttcgacgt gatccgccgt
     3301 gtcgcgaagt caaacgcccc cgttctcatc acgggtgcgt ccgggaccgg aaaagaggtc
     3361 gccgcgcgga cgatccatcg cctttcgaag cgggccgcca aaccattcgt tgccttcaac
     3421 tgcggggcga tctcgccgac gttgatcgag agcgagctct tcggacatga gcgcggctct
     3481 ttcaccggcg cggacaagcg gcgcgtcggt tatttcgagg aggccgccgg cgggacgctc
     3541 ttcctggacg agatcaccga gatggggtcg gaactccagg tgaagctcct ccgcgttctc
     3601 gaaga
//
LOCUS       DASWAV010000375         2128 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U2665
            k141_141935, whole genome shotgun sequence.
ACCESSION   DASWAV010000375 DASWAV010000000
VERSION     DASWAV010000375.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960316
            Sequence Read Archive: SRR10260007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2128)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2128)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:47:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,929
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2128
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_141935"
                     /isolate="SMAG_U2665"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.36444 N 132.31889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            197..388
                     /locus_tag="VF958_07870"
     CDS             197..388
                     /locus_tag="VF958_07870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9149059.1"
                     /translation="MKTTVALAPERWDGLSGAGLLGLFPRRYLMAALVGELLAAIALF
                     ASDNVSPVLVRSLQLFLRF"
     gene            532..996
                     /locus_tag="VF958_07875"
     CDS             532..996
                     /locus_tag="VF958_07875"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF024970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX9149060.1"
                     /translation="MAQRDPAPRPPAVGGAPEVSLTIPVAPEMEIVATAQADALGEYI
                     DMPRDKIDEVKLALVEACINAFEHAQAEDNQVHIMFRVGGRRGAGEWLEIEVLDRGRG
                     FDAAGIQPPTPQKTFEERRRRGWGLHIIKSLMDEVEIESGDGGTRILMRKYK"
     gene            993..1352
                     /locus_tag="VF958_07880"
     CDS             993..1352
                     /locus_tag="VF958_07880"
                     /inference="COORDINATES: protein motif:HMM:NF013868.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HEX9149061.1"
                     /translation="MSETCRVSVERRGDVALFWTDGYINNQGGEEIAREAYAELDRGA
                     RALVMNLEKTRIVNSIGISILIEVLEKVMERKAVLAFCALTPTIDKTFRIMGLAQYAA
                     IYPTEEEALRAVGAPAG"
     gene            1349..>2128
                     /locus_tag="VF958_07885"
     CDS             1349..>2128
                     /locus_tag="VF958_07885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9149062.1"
                     /translation="MSVRADPRVDDLTKALAALRQDSEAPLPVRIAESARSFAGARRA
                     AVFLRHEGDWTSAFDSVGEEPHWDLPERFPDSPEVFDGRLWVPLEAEGEVHGFLALEG
                     LEIDDSAGLAASLGLVFGAVLAASRLSRLVKDAEFELKARLLELESLYDLGLSLSGQL
                     DVSALADEVLYRSISLTDSRKGTLVLFGERGAAPLVRSVGEDLLSADAVRSWDLPEWG
                     GRDQQRRGECPDRRGASERMREVSRGLHRSSRAAPRRARSRG"
BASE COUNT          352 a          688 c          717 g          371 t
ORIGIN      
        1 aagattcctg tattcaaacg cttctccgga aagtcgtttc agaaagtctg gcgtacggcg
       61 acataaagtc acaatacctg cgacgcctct gttccgggtt tgctacccgt aagtcatcgc
      121 ccttcagcga cttccgtcga attccggagg ccctcgcccg gtggcagcgg ccttgcgctg
      181 gaaggtcggc ataaccgtga agaccacagt cgctctcgca cccgaacggt gggacggatt
      241 gtccggtgca ggcctcctcg gcctcttccc gcgacggtat ctgatggccg cgctcgtcgg
      301 cgagcttctg gccgcgattg cccttttcgc ctcggacaac gtctcgccgg tcctcgtccg
      361 ctccctccag ctcttccttc ggttttaggt cccgagatgg accgatctcg aaacaccgag
      421 cttctcgggg cggaggcgga gcgcgcgact cggtcccccg ccgacggcgc ttcggcctcc
      481 gagaaggtat cctcgtcgcg aagggaattc gccacgcgga gagaggccta gatggcgcag
      541 cgcgacccgg cacccaggcc gcccgcggtc gggggagcgc ccgaggtttc cctcacgatt
      601 ccggtcgcgc ccgagatgga gatcgtggcg acggctcagg cggacgccct cggtgagtac
      661 atcgacatgc cgcgggacaa aatcgatgag gtgaagctcg cgctcgtcga ggcctgtatc
      721 aacgctttcg agcatgcgca ggctgaagac aatcaggtgc acatcatgtt ccgggtcggc
      781 ggacggcggg gggcgggcga atggctcgag atcgaagtgc tggaccgcgg gcggggcttt
      841 gacgccgctg gcatccagcc gccgaccccg cagaagacgt tcgaagagcg aaggcggcgc
      901 ggctggggcc tccacatcat caagagcctg atggacgagg tcgaaatcga gtccggcgat
      961 ggaggcacgc gcattctcat gaggaaatac aagtgagtga aacctgcagg gtgtcggtcg
     1021 agagacgtgg cgacgtcgcg ctcttctgga cggacggcta catcaacaac caggggggcg
     1081 aggagatcgc gcgcgaggct tacgcagagc tggatcgcgg ggcgcgcgct ctcgtcatga
     1141 acctggagaa gacgcggatc gtgaattcga tcgggatctc gatcctgatc gaggtgctcg
     1201 aaaaggtcat ggagcggaag gcggtgctgg ccttctgcgc gctcactccc acgatcgaca
     1261 agaccttccg gatcatgggt ctcgcccaat acgccgcgat ctacccgacc gaggaggaag
     1321 ccctccgggc cgtcggcgcg ccagccggat gagcgttcga gcggacccgc gcgtcgacga
     1381 tctgaccaaa gcgctggctg cgctgcgaca agacagcgag gcaccgctcc cggtccgcat
     1441 cgccgaatcc gcccggtcgt tcgccggcgc ccggcgtgcc gccgtctttc ttcgccatga
     1501 gggcgactgg acatccgcct tcgactccgt cggcgaagag ccgcactggg atctcccgga
     1561 gcgatttccc gacagccccg aagtgttcga cgggcggctc tgggtgcccc ttgaggcgga
     1621 aggggaagtc cacggtttcc tggctctcga gggactggag atcgatgact cggccgggct
     1681 cgcagcgtcc ctgggtctcg tcttcggcgc cgtgctggcg gcgagccggc tctcccggct
     1741 cgtcaaggac gcggagttcg agctgaaggc ccgactcctc gagctcgagt ccctctatga
     1801 cctcggcctg tcgctctccg gacagctcga cgtgtcggcc ctcgccgacg aggtgctcta
     1861 ccgctcgatc tctttgaccg attcgcgcaa gggtacgctc gtccttttcg gcgagcgcgg
     1921 cgccgcgccg ctcgtccgga gcgtcgggga agacctgctt tcggccgacg cggtccgctc
     1981 gtgggatctg cccgagtggg gaggtcgcga tcaacaacga cgcggcgagt gtcccgaccg
     2041 ccggggcgcg tctgaacgga tgcgagaagt gtctcgcggt ctccatcgca gttccagggc
     2101 ggcgcctcgg cgtgctcgca gtcgcgga
//
LOCUS       DASWAV010000376         3133 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U2665
            k141_377901, whole genome shotgun sequence.
ACCESSION   DASWAV010000376 DASWAV010000000
VERSION     DASWAV010000376.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960316
            Sequence Read Archive: SRR10260007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 3133)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3133)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:47:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,929
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3133
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_377901"
                     /isolate="SMAG_U2665"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.36444 N 132.31889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..973
                     /locus_tag="VF958_07890"
     CDS             <1..973
                     /locus_tag="VF958_07890"
                     /inference="COORDINATES: protein motif:HMM:NF024045.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="GTPase"
                     /protein_id="HEX9149063.1"
                     /translation="IRDLIDSRTEAAARASARRLEGRLSSRLETAREDLLAAAASLTA
                     TVDFAEDVGEEVDPSVRERLYSARDSLALLAASYETGRLLSAGCRVAILGRPNAGKST
                     LFNALLGTERAIVTDVPGTTRDVLEATLDVSGIPVELVDTAGLREAQDEIERIGVGRA
                     LDEGERAAAVLYVFDSSTGWTAEDGDAIAQLNGKPVAIVANKIDRARGSGGAATPGGP
                     PAALELCGLLPDAGAKLRGLLEETVASDLVSDATSDVLASVRQRDLVARASARAGEAI
                     DALARGVSPEYASTHVDAALAALADVLGETTPDEVLERIFSTFCIGK"
     gene            970..2847
                     /gene="mnmG"
                     /locus_tag="VF958_07895"
     CDS             970..2847
                     /gene="mnmG"
                     /locus_tag="VF958_07895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008943230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HEX9149064.1"
                     /translation="MNGVARFDVLVVGGGHAGCEASHAAARMGLKTALLTGDRRAIAR
                     MSCNPAIGGLAKGQLVSEIDALGGLMGRLADRAGIQFKILNRSRGPAVWGPRAQCDKP
                     LYSRLAAETLAATPNLRIVEGMAEDFLATGGRVAGVVTGAGERIESEAVVVTTGTFLR
                     GLMHTGARQTSGGRFGEPAAEGLSAALAKVGLRLGRFKTGTPPRVHRDSVDYDLCEPQ
                     RGDDPPVPFSFRTESLPAGQALCWLTATNERVHEIIRANLHASPLYSGRIVGIGPRYC
                     PSVEDKVVKFADKPRHSIFLEPEGWNVEEIYINGLSTSLPEEVQREILLAIPGLSHAR
                     MLRPGYAVEYDFSFPDQLRATLEARDVPGLFLAGQINGTSGYEEAAAQGIVAGINAAL
                     AVRRDEPFVLERSEAYAAVMIDDLTTKGLEEPYRLFTSRAEYRLLLGVDSVLPRLLPH
                     ALRLGLVSLEEYSRAMRSEDRLRRAESALAEAVIRPDRETVAGMERALGVRIEEPTTL
                     HKLLKRQDLTAEALAGWAPEIFSTLSREERAVLEARVRYEGYIRRELERVERLRPLES
                     RRIPADFDYELPGLSREVIEKCARRRPGTVGEAGRIPGVTPAAVAIICAHVGRARTPS
                     A"
     gene            2816..>3133
                     /locus_tag="VF958_07900"
     CDS             2816..>3133
                     /locus_tag="VF958_07900"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RsmG family class I SAM-dependent
                     methyltransferase"
                     /protein_id="HEX9149065.1"
                     /translation="MSVGREPLPPETFHGLLGRELPRFGLEPSPDVVDRLARFLAELD
                     LWRRRINLTGRLSATELVAHAAESVLGGRFLPPGARVIDVGTGGGFPGVPLAIARPDL
                     EMTW"
BASE COUNT          488 a         1086 c         1071 g          488 t
ORIGIN      
        1 gatccgcgac ctcatcgatt cgcgcaccga ggcggcagcc cgtgcctcgg cgaggagact
       61 cgagggccgc ctctcgtcgc gcctcgagac agcgcgggaa gatctcctcg cggccgcggc
      121 gagtctgacg gcgacggtgg acttcgccga ggacgtcggc gaggaggtcg acccctccgt
      181 ccgtgagcgt ctctactcag cgcgagattc cctcgcgctt ctcgccgcga gctacgaaac
      241 cggccgcctc ctctccgccg gctgccgagt cgcgatcctc ggccgcccca acgcggggaa
      301 gtcgacgctc ttcaacgccc tcctcgggac ggagcgcgcg atcgtgacgg acgtgccggg
      361 cacgacgcgc gacgtcctcg aagcaacgct cgacgttagc gggatcccgg tggagctggt
      421 cgacacggca ggtctgcgcg aagcgcagga cgagatcgag cgaatcggcg tcggacgcgc
      481 gctcgacgag ggagaacggg cggcggccgt cctgtacgtg ttcgactctt cgaccggatg
      541 gacggccgaa gacggggacg cgatcgcaca actgaacggg aaaccggtcg cgatcgtggc
      601 gaacaagatc gaccgcgccc gcgggtccgg cggagccgcg acgcccggcg ggcctccagc
      661 ggccctggag ctctgcggtc tccttccgga cgcgggagcg aaactccgcg gactcctcga
      721 ggaaaccgtc gcgtctgacc tcgtgagcga cgccacctcc gacgtcctcg cgtccgtgcg
      781 ccagcgcgat ctcgttgccc gcgcctcggc tcgcgccggc gaggcgatcg acgcgctcgc
      841 gcgaggcgtg tcgccggaat acgcgtcgac gcacgtcgac gccgcgctcg cggcgctcgc
      901 cgacgttctc ggtgaaacga ctcccgacga agttctcgag cggatcttct cgacgttctg
      961 tatcggaaaa tgaacggcgt ggcgcgattc gacgttctcg tggtcggggg cggacacgcc
     1021 gggtgcgagg cctctcacgc cgccgcgcgc atgggtctga agacggcgct gctgacgggt
     1081 gatcgccggg cgatcgcacg gatgtcctgc aatccggcga tcggcggcct ggcgaagggc
     1141 cagctcgtct ccgagatcga cgcgctcggt ggactgatgg gacgtctcgc cgaccgcgcc
     1201 ggcatccagt tcaagattct caaccgttcc cgaggcccgg cggtgtgggg tccccgggcg
     1261 cagtgcgaca aacctctcta ctcacggctc gctgcggaaa ccctcgccgc tacgccgaat
     1321 ctccggatcg tcgagggcat ggcagaagac ttcctggcga cgggcggccg cgtcgccggc
     1381 gtcgtgaccg gcgcgggcga acgcatcgaa tcggaagccg ttgtcgtcac gacgggaact
     1441 ttcctgcgcg gattgatgca cacgggcgcc cgccagacgt cgggtggccg cttcggtgag
     1501 ccagctgcag aggggctttc ggcggcgctc gcgaaggtcg gtttgcgcct ggggcggttc
     1561 aagaccggca cgccgccgcg cgtccaccgg gactccgtgg actacgacct ctgcgaaccg
     1621 caacgcggcg acgatccgcc ggtcccgttc tctttccgca ccgagagcct tcccgccgga
     1681 caggcgctct gctggctgac cgcgacgaac gagcgcgtgc acgaaatcat tcgcgccaac
     1741 ctgcacgcga gcccgctgta ctccgggagg atcgtgggca tcggtccgcg ctactgccca
     1801 tcggtcgagg acaaagtcgt caagttcgcg gacaagccgc gacactcgat ttttctcgag
     1861 ccggaggggt ggaacgtcga ggagatctac atcaacggtc tctcgacgtc gctgcccgag
     1921 gaagtgcagc gcgagatcct gctcgcgatc cccggactct cgcacgcgcg catgcttcgc
     1981 cctggatacg cggtcgaata cgacttctcc ttccccgacc agttgcgcgc gacgctcgag
     2041 gcccgagacg tgccgggact ctttcttgcg ggacagatca acggcacgtc cggatacgaa
     2101 gaagccgcgg cgcaggggat cgtcgccggc atcaacgcag cgctcgcggt ccgccgcgac
     2161 gagccattcg ttctcgagcg ctcggaggcc tacgcagcgg tgatgatcga cgacctcacg
     2221 acgaaggggt tggaggaacc gtaccgcctc ttcacctcgc gcgccgagta ccgcctgctc
     2281 ctcggcgtcg actccgtgct gccacggctc ctgccgcacg cgcttcggct gggactggtc
     2341 tctctcgagg agtactcgcg cgcgatgcgc tcggaggatc ggctccggcg agccgagtcg
     2401 gcactcgccg aggccgtcat ccggccggac cgcgagaccg ttgcggggat ggagcgcgcg
     2461 ctcggggtcc ggatcgaaga acccacgact ctgcataaac tattgaaaag acaggactta
     2521 acagccgaag ccctggccgg ctgggcgccc gagatcttct cgacattgag ccgcgaggag
     2581 cgggcggtcc tcgaggcccg ggtccgctac gaaggttaca tccggcggga actggagcgc
     2641 gtggagcggc tgaggccgct ggagagccgg cggattcccg cggatttcga ctacgaactt
     2701 cccggccttt cccgcgaagt catcgagaag tgcgcgcgcc gacgtcccgg cacggtcggg
     2761 gaagccggac gtatccccgg cgtcaccccc gcggcggtcg cgataatctg cgcgcatgtc
     2821 ggtcgggcga gaaccccttc cgcctgagac ttttcacggc cttctcggcc gcgaactccc
     2881 ccgcttcggc ctggagccgt cgcccgacgt cgtcgatcgg ctcgcccggt ttctcgccga
     2941 actcgacctc tggcgccgcc ggatcaacct gacaggaagg ctttccgcca ccgagctcgt
     3001 tgcgcacgcg gccgagtcgg tgctgggcgg gagattcctt ccgccgggcg cgcgggtcat
     3061 cgatgtcggg accggtggcg gatttcccgg agtcccgctc gcgatcgcaa ggcccgacct
     3121 cgagatgacc tgg
//
LOCUS       DASWAV010000377         1993 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U2665
            k141_659617, whole genome shotgun sequence.
ACCESSION   DASWAV010000377 DASWAV010000000
VERSION     DASWAV010000377.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960316
            Sequence Read Archive: SRR10260007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 1993)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1993)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:47:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,929
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1993
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_659617"
                     /isolate="SMAG_U2665"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.36444 N 132.31889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(154..1101)
                     /locus_tag="VF958_07905"
     CDS             complement(154..1101)
                     /locus_tag="VF958_07905"
                     /EC_number="1.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012470354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase"
                     /protein_id="HEX9149066.1"
                     /translation="MNSALEARLGPLTLKNPIVTASGTFGYGLEFTDFVDLSKIGALC
                     TKGLSLAPHAGNMPPRICETPAGMLNAIGLQNVGVQAFLDEKLPRLAELGATVIANVW
                     GDTEEEYVAVVGALEDAAGLAAIELNISCPNVAKGGMLFGNSPAATASLVGRVRAATR
                     RTLVVKLSPNAPDLIESARAARDSGADVISLVNTFVGMAIDPVTAQPRLSFGTGGLSG
                     PAIKPLAVRMVFQVARALPDVPIMGIGGISDLSDVLEYLAAGASAVQIGTANFRDPAV
                     SGRLVDELEAYCRNRETTVAALVGRAQRTSSDGKAGAEG"
     gene            complement(1098..1895)
                     /locus_tag="VF958_07910"
     CDS             complement(1098..1895)
                     /locus_tag="VF958_07910"
                     /inference="COORDINATES: protein motif:HMM:NF012402.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase electron transfer
                     subunit"
                     /protein_id="HEX9149067.1"
                     /translation="MKADHRASLIARHVFFPGFFSATFRAPALARDVLPGQFVMLEVA
                     GRLRPYLRRAYSVADAEPATGEVEFLVKTIGPGTAALEDLAEGSGTSLLGPLGNGFSL
                     AGLPSAARVAIVAGGIGAAPFPLLLRAMGKAGVSADLFLGGRNARELGFRDRFAGLVP
                     GETLLSTDDGSLGEKGFVTDAFARRAAATPYSRVYACGPMPMFVALARVVERAGLPAE
                     FSTEAAMGCGFGACLGCVIPGREKPFVISCMEGPILPPEKIDWTKVR"
BASE COUNT          332 a          704 c          682 g          275 t
ORIGIN      
        1 tgttcgcgac gtcggagacg atgccggggc cctcggccgt gaacgcctcg agtccgacct
       61 tcagccaccc ggctttggcg ccgaagcggc gcgccgccgc gaggatctcc tcgcgcgtcg
      121 cgaaatcgag cgcgatgcag aggcgctctt ctctcaccct tcggcccccg ctttcccgtc
      181 gctcgaggtg cgctgcgcgc gcccgacgag ggccgcgacc gtcgtctcgc gattgcggca
      241 gtacgcctcg agctcgtcga cgagcctccc ggacacggcg gggtcccgga agttcgctgt
      301 gccgatctgc acggcggaag cacccgcggc gaggtactcg aggacgtcgg aaaggtccga
      361 gatcccgccg atccccatga tcggaacgtc cggcagcgcg cgcgccacct ggaacaccat
      421 gcgcacggcg agaggcttga tcgcggggcc ggagagtccg ccggtgccga acgaaagccg
      481 cggctgcgcc gtgaccggat cgatcgccat cccgacgaac gtgttgacga gcgagatcac
      541 gtccgcgccg gaatcgcgcg cggcgcgagc cgattcgatc aggtcgggcg cgttcggaga
      601 gagcttgacg acgagggtcc gccgggtcgc cgcccggacc cggccgacga gcgaggcggt
      661 tgcggccggt gagttcccga agagcattcc ccccttcgcg acgttcggac aggagatgtt
      721 gagctcgatt gcggcgagtc cggcggcgtc ttccagcgcg ccgacgaccg cgacgtactc
      781 ctcttccgtg tccccccaga cgttcgcgat caccgttgcg ccgagctcgg cgagccgcgg
      841 aagtttctcg tccaggaacg cctgcacgcc gacgttctgg agtccgatcg cgttcagcat
      901 tccggcgggc gtctcgcaga tgcgcggcgg catgttgccg gcgtgcggcg cgagagagag
      961 ccctttcgtg cacagcgcgc cgatcttcga aagatcgacg aagtcggtga actcgagccc
     1021 gtaaccgaac gtccccgacg ccgtgacgat cgggttcttc agcgtgagcg ggccgagccg
     1081 ggcttccagt gcggagttca tcggaccttc gtccagtcga ttttctccgg cggcaggatc
     1141 ggcccctcca tgcacgagat cacgaacggc ttctcgcgcc ccgggatcac gcacccgagg
     1201 cacgcgccga acccgcagcc catcgccgcc tccgtcgaga actcggcggg aaggccggcc
     1261 cgctcgacga ccctcgcgag cgcgacgaac atcggcatcg ggccgcacgc gtagacccgc
     1321 gagtacgggg ttgccgcggc gcggcgggcg aaggcgtcgg tgacaaaacc cttctctccc
     1381 agcgagccgt cgtcggtcga aaggagggtc tcgcccggga ccaggcccgc gaagcggtcg
     1441 cgaaagccga gctcgcgcgc gttgcggcct cccaggaaga gatcggcgga cacgcccgcc
     1501 ttccccatcg cgcgaagcag cagcggaaat ggcgccgcgc cgattccgcc cgcgacgatc
     1561 gcgacccgcg cggcagaagg aagccccgcg agcgagaacc cgttgccgag cggcccgagg
     1621 agagacgtgc cggatccctc cgccaggtcc tcgagcgccg ccgtgcccgg cccgatggtc
     1681 ttgacgagga actcgacctc tccggttgcc ggctccgcgt ccgcgaccga gtacgcccgg
     1741 cgcaggtagg ggcgcaaacg ccccgcgacc tcgagcatca cgaactgtcc gggaaggaca
     1801 tcgcgcgcaa gagcgggagc tcggaatgtt gcggagaaga atccgggaaa gaagacgtgc
     1861 cgggcgatga gagacgcccg atgatcggcc ttcacgggcg aaagctatca gctccgcccg
     1921 ggagccgaaa cgccgttaaa ggatccgacc gaactcgccg acggacccga ggtccttctt
     1981 ctccgactcg tcg
//
LOCUS       DASWAV010000378         3119 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U2665
            k141_501015, whole genome shotgun sequence.
ACCESSION   DASWAV010000378 DASWAV010000000
VERSION     DASWAV010000378.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960316
            Sequence Read Archive: SRR10260007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 3119)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3119)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:47:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,929
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3119
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_501015"
                     /isolate="SMAG_U2665"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.36444 N 132.31889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1806
                     /locus_tag="VF958_07915"
     CDS             <1..1806
                     /locus_tag="VF958_07915"
                     /inference="COORDINATES: protein motif:HMM:NF016289.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family peptidase"
                     /protein_id="HEX9149068.1"
                     /translation="ALFSAEESGIAGSSYFVAHSPVALERVRAMVNLDMVGALRDDQL
                     IAFGAESAPEWTAALESAAKPLGLKLTSKGDGYGPSDQTAFYASKIPVVHFFTGAHDR
                     YHTPEDDAGAINFAGGGRVAEMTARVGELLALGKLTPTYAVAAAAPAMQGDSRGYGAY
                     LGTIPDYRAMEDSKGGVLLGDVRPGGPADHAGIRGGDRIVEMAGTKIENLYDMTFALQ
                     DHKPGETIDVVVVRNGERVTLRATLGERRGAEPGANPPAHPAPPTPSPGSPGPGIGAG
                     KPFAARFDGERHLQDVRQLTFGGENAEAYFSPDGAKIIYQSTPPDASCDQQFVMDLAT
                     GESKRVSSGKGRTTCGYFRFPQGDRIVYASTEAAGAECPAPPDRSKGYVWAVYDGYDL
                     YEAKPDGSEARRLTQTPGYDAEATWCPKGGKLVFTSARDGDLDLYEMTESGSVKRLTN
                     TPGYDGGAFYSPDCSEIVWRASRPTGAALTEFRDLLAKGLVRPTALELFLAKADGTAA
                     RQLTHNGAANFCPYFHPDGKRIIYSSNAGSPDGREFDLWLLDKNGGASERVTTAPGFD
                     GFPQFSPDGKWIVWGSNRANPKGHETNLFLARWVE"
     gene            complement(2137..2706)
                     /locus_tag="VF958_07920"
     CDS             complement(2137..2706)
                     /locus_tag="VF958_07920"
                     /inference="COORDINATES: protein motif:HMM:NF024827.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c family protein"
                     /protein_id="HEX9149069.1"
                     /translation="MKTLSRVLILAVGFLLLASVAAASASYLGAKKCKACHIKQFQSW
                     EKTRMAHAFELLSPGAAAAAKKKANLEPQKDYTHDQKCIGCHTTGFGSPGGFVSFEQT
                     PELVGVQCESCHGPGGEYTKPGGMTLQNKEYHRADLVKLGLVVVSAETCTTACHNNRS
                     PFVGKDYVFDYAKRRTEGTHEHIALKYAH"
     gene            complement(2742..>3119)
                     /locus_tag="VF958_07925"
     CDS             complement(2742..>3119)
                     /locus_tag="VF958_07925"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HEX9149070.1"
                     /translation="WPAMSEAMESVGMSPPSFQGDEMADLFAYLFLSRYDGGPGDVAR
                     GKGIYLQRGCTACHGQNGEGNIGPALRTVTTGEAKERIAERMWNHAEKMSEKMNAYSV
                     PWPHLDSGEMADLFTFLAEGWKR"
BASE COUNT          535 a         1048 c         1024 g          512 t
ORIGIN      
        1 gccctcttct ccgccgagga gtcgggaatc gccggttctt cgtacttcgt cgcccattcc
       61 cccgtcgccc tcgagcgtgt ccgcgcgatg gtcaatctcg acatggtcgg agccctccgc
      121 gacgaccagt tgatcgcgtt cggcgcggag tccgcaccgg agtggaccgc ggcgctggaa
      181 tccgccgcga aaccgctcgg gctgaagttg acatcgaagg gggacggcta cgggccctcg
      241 gaccagaccg cgttctatgc gtcgaaaatc ccggtcgtcc acttcttcac gggcgcgcac
      301 gatcgctacc acacccccga ggatgacgcc ggcgcaatca acttcgccgg aggcggccgt
      361 gtcgccgaaa tgacggcgcg cgtgggtgag cttctcgcgc tcggaaagct cacgcccacg
      421 tacgccgtgg ccgctgccgc accagcgatg cagggcgaca gccggggcta cggcgcttac
      481 ctcggcacca tccccgacta ccgggcgatg gaagactcga agggcggcgt gctcctgggt
      541 gacgtacggc cgggaggccc cgccgatcac gccggaatcc gcggaggcga tcgcatcgtc
      601 gagatggcgg gcacgaaaat cgagaacctc tacgacatga cgttcgcgct gcaggaccac
      661 aagcccggcg agacgatcga cgtcgtcgtg gtccgaaatg gcgagcgggt gacgctgcgc
      721 gcgaccctcg gcgagcggcg gggggcggaa ccgggagcga accctcccgc gcatcccgcg
      781 cctccgacgc cgtcccccgg cagcccgggt cccgggatcg gcgccggcaa accgtttgcg
      841 gcgcggttcg acggcgagcg gcacctgcag gacgtccggc aactcacctt cggcggcgag
      901 aacgccgagg cgtacttctc ccccgacggc gcgaagatca tctaccagtc gacccctccg
      961 gatgcgtcct gcgatcaaca gttcgtgatg gacctcgcga ccggagaatc gaagcgcgtc
     1021 tcgagcggaa agggccgcac gacctgcggg tatttccggt tcccgcaggg cgaccggatc
     1081 gtctacgcgt ccacggaagc cgctggcgca gagtgtccgg cgcccccgga ccgttcgaag
     1141 ggatacgtct gggccgtgta cgacggctac gacctctacg aggcgaagcc ggacggttcg
     1201 gaggcgcgga ggctcaccca gacgcccggt tacgacgcgg aagcgacgtg gtgcccgaaa
     1261 gggggcaagc tcgtcttcac gtccgcgcgc gacggcgacc tcgacctcta cgagatgacc
     1321 gagtccggaa gcgtgaagcg actgacgaac actcccggat acgacggggg cgccttttac
     1381 agcccggact gctccgagat cgtgtggcgg gcgagccggc cgaccggcgc cgcgctgacg
     1441 gagttccggg atctgctcgc gaagggcctc gtccgtccga ccgcactcga gctctttctc
     1501 gcgaaagcgg acgggaccgc tgcgcgacag ctcacccaca acggcgccgc gaacttttgc
     1561 ccgtacttcc atcccgacgg gaaacggatc atctacagct caaacgcggg atcgcccgac
     1621 ggccgcgagt tcgatctctg gctgctcgac aagaacggcg gcgcctccga acgcgtgacg
     1681 accgcgccgg gcttcgacgg cttcccgcag ttctcccccg acggcaagtg gatcgtctgg
     1741 ggctcgaacc gggcgaaccc gaagggtcac gagacgaacc tgttcctcgc ccgctgggtc
     1801 gagtaagcgg ttttcgaagg aggagagccc ctcacccgac cctctccccg tcaccgggga
     1861 gaggggaaga ctcggtcgcg gcgcgaggag tctcaccgtt cgcatcgcga agagtctccg
     1921 cgtagttcgc ggcgcgcgga gtctcccgag gtaatcgagg gaggagcctt cgacgcgggt
     1981 tcgcatcgcg aagagtctcc gcgaagtaac cgagcggagg agcttcgcga tgcgggagcg
     2041 cgagcgcagc gggcgaccag gcacgcggtc gaggccggcc tgccgagccg cgtgccggtg
     2101 cgcgagcagg tcgctcgcaa gcgagcgcga cggaaatcag tgcgcgtact tcagagcgat
     2161 gtgttcgtgc gtcccctcgg ttcgacgctt cgcgtagtcg aagacgtagt ccttcccgac
     2221 gaacgggctc cggttgttgt ggcaggccgt cgtgcacgtc tccgcgctga cgactacgag
     2281 cccgagcttg accagatcgg cgcgatggta ttccttgttc tgaagtgtca tccctcccgg
     2341 tttggtgtac tcacctcccg gtccgtggca cgattcgcac tgcacgccga ctaattccgg
     2401 cgtctgctcg aagctgacga atccgcccgg agacccgaac cccgtcgtgt ggcacccgat
     2461 gcacttctgg tcatgggtgt agtccttctg aggttcgagg ttcgccttct tcttcgccgc
     2521 ggccgctgca cccgggctga gcagctcgaa tgcgtgggcc atccgggtct tttcccagga
     2581 ctgaaactgc ttgatgtgac acgccttgca tttctttgcg ccgaggtaag aggcagacgc
     2641 agcagctact gacgcaagca gcaggaatcc gaccgcgaga atcaggacac gcgaaagggt
     2701 cttcatcgac tctcctccat tcccagacac aggttcgaac ttcaccgttt ccaaccctcg
     2761 gcgagaaagg tgaagagatc ggccatttcc ccggagtcga ggtggggcca ggggacgctg
     2821 taggcgttca tcttttcgct catcttctct gcgtggttcc acatgcgctc ggcgatgcgc
     2881 tccttcgcct ctccggtcgt tacggtgcgg agcgccggcc cgatgttgcc ctcgccattc
     2941 tgaccgtggc atgcggtgca gccgcgctga aggtagatcc ccttcccgcg ggcgacgtct
     3001 cccggaccgc cgtcgtatcg ggagaggaag agatacgcga agagatccgc catctcatcg
     3061 ccctggaacg acggtggcga catgccaacg gactccatgg cctccgacat cgcgggcca
//
LOCUS       DASWAV010000379         2520 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U2665
            k141_142319, whole genome shotgun sequence.
ACCESSION   DASWAV010000379 DASWAV010000000
VERSION     DASWAV010000379.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960316
            Sequence Read Archive: SRR10260007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2520)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2520)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:47:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,929
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2520
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_142319"
                     /isolate="SMAG_U2665"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.36444 N 132.31889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..719)
                     /locus_tag="VF958_07930"
     CDS             complement(<1..719)
                     /locus_tag="VF958_07930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015719581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005267 - potassium channel activity
                     [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium channel family protein"
                     /protein_id="HEX9149071.1"
                     /translation="MRAWAIAAGLVLIALVLWDAFETIILSRRVSRKVRLATFFYRLT
                     WPAWRALGRRMRPGNSRENYLSVYGPLSLIGLLVLWAVSLISSFAMLLWGLNAPIRQV
                     VGTEGFPEYLYFSGSTFFTLGLGDVAPLSTDGRALSVAEAGTGFAFLALMISYLPILY
                     QAFSRREVDIALLDARAGSPPSAAELLRRHAGDSESLGHLLLDWEKWSAQLLESQISF
                     PVLAYFRSQHDNQSWLAGLTM"
     gene            759..2105
                     /gene="allB"
                     /locus_tag="VF958_07935"
     CDS             759..2105
                     /gene="allB"
                     /locus_tag="VF958_07935"
                     /EC_number="3.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020738891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004038 - allantoinase activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0050897 - cobalt ion binding [Evidence
                     IEA];
                     GO_process: GO:0000256 - allantoin catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="allantoinase AllB"
                     /protein_id="HEX9149072.1"
                     /translation="MIRSRRVVTPEGVRPASIFVDGEVIAGVEPGAASVPAGFVLFDF
                     GDRVVMPGLVDTHVHVNEPGRTDWEGFETATRAAAAGGTTTLFDMPLNSVPPTTSVAA
                     LREKAAAAEGKCFTDVGFWGGVVPGNTGELAAMADRGVPGFKCFLVPSGVPEFGFVSA
                     RELSEALSEIARIGSVLLVHAEDPRRIGRAPAGDPRKYTTYLATRPSGAEDDAVALLA
                     RLARETGARIHVVHLSSAEAIAAVETARKNGLSLSAETCPHYLFFAAEDVPDGATEFK
                     CAPPIRERANRERLWKGVADGAIGMVVSDHSPSPTEMKSRETGDFLAAWGGISSLQFR
                     LPAVWTEARERGHSIERLAAWMCEAPARLAGLAGRKGSIVPGADADLVVWDPESSFEV
                     RAEATFHRHGLTPYLGRTLSGVAEATFLRGEKIFERGHFVGPPRGRVLVREILEGA"
     gene            2102..>2520
                     /locus_tag="VF958_07940"
     CDS             2102..>2520
                     /locus_tag="VF958_07940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013132824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional allantoicase/OHCU decarboxylase"
                     /protein_id="HEX9149073.1"
                     /translation="MTDFTELVDLASERLGGAVIAASDEFFAPKENLLKAAKPVFLED
                     EYTDRGKWMDGWETRRRREPGHDWCVVRLGRPGNIRGVNVDTAFFRGNVPESCSVEAA
                     ESAEGPWTELLPRSPLSGDAENLFPIESGRRYTHVKL"
BASE COUNT          427 a          797 c          905 g          391 t
ORIGIN      
        1 atcatcgtca atccggcgag ccaggactgg ttgtcgtgct gcgagcggaa gtaggcgagc
       61 accgggaagg agatctggct ctcgaggagc tgtgccgacc atttctccca gtcgagcagg
      121 agatggccga gcgactccga atctccggcg tggcgccgaa gaagctcggc cgcgctcggc
      181 ggcgacccgg cgcgtgcgtc gagcagcgcg atatcgactt cgcggcggga aaacgcctgg
      241 tacaggatgg gcaggtagct gatcatgagg gcaaggaacg cgaatcccgt cccggcctcg
      301 gcgaccgaga gggcgcgacc gtcggtcgag agcggtgcga cgtctccgag cccgagggtg
      361 aagaaggtcg accccgagaa gtagaggtat tccgggaacc cttcggttcc gacgacctgg
      421 cgaatcggtg cattcaagcc ccagaggagc atcgcgaacg aagagatcag cgagacggcc
      481 cagaggacga gcagtccgat gagcgaaagg ggaccgtaga cgctcaggta gttctcccgg
      541 gagttgccgg gccgcatccg ccgcccgagt gcacgccacg ccggccacgt cagccgatag
      601 aagaaagtcg cgagccgcac cttccgtgag acgcggcgcg agagaatgat cgtctcgaaa
      661 gcatcccaga gaaccaatgc gatcaggacg agtcccgcgg cgatcgccca cgccctcacg
      721 ggccgaattg aatcattgaa ttgagcggcg cgggttcggt gatccggagc cggcgggtcg
      781 tcacgccgga gggcgtgcga ccggcgtcga tcttcgtgga cggcgaagtc atcgccggag
      841 tcgagcccgg ggcggcgtcg gttcccgcgg gcttcgtcct cttcgatttc ggcgaccgcg
      901 tcgtgatgcc ggggctcgtg gacacgcacg tccacgtcaa cgagcccggg cggaccgatt
      961 gggagggttt cgagacggcg acgcgggcgg cggccgccgg gggaacgacg acgctcttcg
     1021 acatgccgtt gaacagcgtt cccccgacaa cctcggtcgc ggctctccgc gagaaggcgg
     1081 cggcggccga gggaaagtgt ttcaccgacg tcggattctg gggcggcgtc gtccccggca
     1141 acacggggga gctcgccgcg atggcggatc gcggcgtgcc cggattcaag tgctttctcg
     1201 tgccgtcggg cgtgccggaa ttcgggttcg tatccgcgag ggaactctcg gaagcactct
     1261 ccgagatcgc gcgcatcggg tcggtcctcc tcgtgcacgc ggaggatccc cgccgcatcg
     1321 ggagagcgcc ggcgggcgat ccccgaaagt acacgacgta tctcgcgacg cgcccttcgg
     1381 gcgcggaaga cgacgcggtc gctcttcttg cgcgactcgc ccgcgagacg ggcgcgcgga
     1441 tccacgtcgt ccacctctcg tcggccgaag cgatcgccgc ggtcgagacc gctcgaaaga
     1501 atggactgtc gctgtcggcg gagacgtgtc ctcactatct cttcttcgcg gcggaggacg
     1561 tgcccgacgg cgcgacggag ttcaagtgtg cgccgccgat ccgggaacgg gcgaaccggg
     1621 agcggctgtg gaagggcgtg gcagacggcg caatcgggat ggtcgtctcc gaccactctc
     1681 cttcccccac ggagatgaag agccgcgaga cgggggactt tctcgccgcc tggggaggga
     1741 tctcatcgct tcagttccgg ctccccgcgg tgtggacgga ggcgcgcgaa cgcggccact
     1801 cgatcgaacg cctcgccgcg tggatgtgcg aagcgccggc gcggctcgcc gggctcgcgg
     1861 ggcgaaaagg ctcgatcgtt cccggtgccg atgcggatct cgtcgtgtgg gatccggaga
     1921 gctccttcga ggtgcgtgcc gaagcgactt tccaccgaca cggtctgacc ccttatctcg
     1981 ggcgcacgct ctccggcgtc gccgaagcga ctttccttcg cggtgagaag atcttcgagc
     2041 gcggccactt cgtgggacca ccgaggggaa gagttctcgt tcgcgaaatc ctggagggcg
     2101 cttgacggac ttcaccgagc tcgtggatct ggcttcggag cgcctcgggg gcgccgtgat
     2161 cgccgccagc gacgagttct tcgctcccaa ggagaatctc ctcaaggccg cgaagcccgt
     2221 gttcctcgaa gacgagtaca ccgaccgcgg caagtggatg gacggatggg agacgcggcg
     2281 tcggcgagag cccggccacg actggtgcgt cgtccggctg ggccggccgg gaaatatccg
     2341 tggcgtgaac gtggacacgg cgttcttccg cggcaacgtc ccggagtcgt gctcggtcga
     2401 ggcggcggag tcggcggaag gcccctggac cgaactcctg ccgcgctcgc cgctctcggg
     2461 tgacgcggag aatctcttcc cgatcgagag cggccggcgc tacacccatg tgaagcttca
//
LOCUS       DASWAV010000380         2727 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U2665
            k141_621641, whole genome shotgun sequence.
ACCESSION   DASWAV010000380 DASWAV010000000
VERSION     DASWAV010000380.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960316
            Sequence Read Archive: SRR10260007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2727)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2727)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:47:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,929
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2727
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_621641"
                     /isolate="SMAG_U2665"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.36444 N 132.31889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..574)
                     /locus_tag="VF958_07945"
     CDS             complement(<1..574)
                     /locus_tag="VF958_07945"
                     /inference="COORDINATES: protein motif:HMM:NF012610.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEX9149074.1"
                     /translation="MTARPAAEIDFVNSLGAWSQGSGPFYRRLANGLRSAILRGDLPP
                     GARVPAERNLARLLAVSRTTVVSAYEILRQEAWLESRQGSGTRVRPAPRRSQTRGAEP
                     PSFRRNPVYRSLIEGSGGTIEFLGAHLPGTGAIPEEILSLRDGVARLARGHGYLPAGL
                     PELRRAIAKRLDARGLPTKEDEVLVTSGAQQ"
     gene            696..1685
                     /locus_tag="VF958_07950"
     CDS             696..1685
                     /locus_tag="VF958_07950"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012190895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004084 - branched-chain-amino-acid
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009081 - branched-chain amino acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transaminase"
                     /protein_id="HEX9149075.1"
                     /translation="MALPGYAFFKGRIVPYSEARVGVLTHGLNYGTGVFSGIRGYWNP
                     DEKELYVFRPEDHYRRFLESARMLGMELPWNARQLSGAAAELIRAEDHREDCYLRSLA
                     FYGDETVGVRLHDLTPEVSIVAIPYGRYIEKEEGAHATISSWRRVDDNAIPARGKIVG
                     AYVNSALAKSEAMRAGFDDAIVLTQDGHVSEGSAANFMLVRDGVVITPPITDNILEGI
                     TRRTVMELLDKEMGVTVLERSIDRSEIYVADEAFFVGTGVQIVAITRVDHRPIGNGRI
                     GPIGTALRDLYFRVVRGRHPAYQAWCSGVYGGAAAREKTAMRPAEPERTAAAR"
     gene            complement(1710..>2727)
                     /locus_tag="VF958_07955"
     CDS             complement(1710..>2727)
                     /locus_tag="VF958_07955"
                     /inference="COORDINATES: protein motif:HMM:NF019982.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX9149076.1"
                     /translation="YNTVESARDIEDLRRALGVPRVSLLAGSYGSHLALATIRLFESS
                     IERAVLFGVQGPDHMERLPGDFQQVLVQIDEMVKRTPLLAERMPSFLGVVQTVLSKLE
                     REPIRVESVDAGTGEHRTVTLGKFDLQCFTRNMLSSREQISRLPGLFLAMLRGDFQEL
                     AASSIAGRKSAAPPAVDFTMRCASGGSSARRARVAAQAQSALLGDAADFPIPAVCDAW
                     GVPDLGEDFRAPISSSVPVLFVSGTLDAHTPTSNAREVLAGFSNGRLLVVDGGAHCLL
                     GFSLPKGRKITAKFLRGDSVSTARLSSPAFGFFVPELSREGMVAAEFGRSLRAPLGYI
                     PLPH"
BASE COUNT          472 a          896 c          940 g          419 t
ORIGIN      
        1 cctgctgcgc gccgctcgtg acgaggactt cgtcttcctt cgtcggaaga ccgcgcgcgt
       61 ccaaccgctt cgcgattgcc cgccgcagct ccggaagccc cgccggcaag tagccgtggc
      121 cgcgcgcgag ccgcgcgacg ccgtctctca gcgaaaggat ctcttcgggg atggcgccgg
      181 ttccggggag gtgggcgccg agaaactcga tcgtgccgcc ggacccttcg atgaggctcc
      241 ggtaaaccgg atttcgccgg aaagacggcg gctcggcgcc gcgggtctgc gaccgccggg
      301 gagccggacg cacccgcgtc ccgctgccct ggcggctttc gagccaggcc tcctgccgga
      361 ggatctcgta cgcggaaacg acggtcgtgc gggaaacggc aaggagccgc gcaaggttac
      421 gctcggcggg gacgcgtgcc ccgggtggga gatcgcccct caggatcgcc gagcgcagtc
      481 cattggcgag acgccgataa aaagggccgg aaccctgtga ccatgcgccc agagagttca
      541 cgaagtcgat ctcggcggca ggtcgtgcgg tcatcggtca gtccacttct tctgaagtgg
      601 ctattgatcc tagcagaact gtcgtcggat gctccccctg gttcgaggcg gatgagaatt
      661 ggactcatcg attggccttg gaccggggag gtcgcatggc tcttccggga tacgccttct
      721 tcaagggccg gatcgttccg tactccgaag cgcgagtcgg agtcctcacg cacggcctga
      781 actacggaac cggggtattc agcggaatac gcggttactg gaaccccgac gagaaggagc
      841 tctacgtatt ccggccggaa gaccattacc ggcgattcct cgagagcgcc cggatgctcg
      901 ggatggagct cccgtggaac gcgcggcagc tctcgggagc ggcggccgag ctcattcgtg
      961 ccgaggatca ccgcgaagac tgctacctcc gctcgctcgc gttctacggc gatgaaaccg
     1021 tcggcgtccg acttcacgac ctgacgcccg aggtctcgat cgttgcgatt ccttacggcc
     1081 gctacatcga gaaggaggag ggcgcgcacg ccacgatttc gtcctggcgg cgtgtggacg
     1141 acaacgcgat cccggcgcgg ggaaagatcg tcggggcgta cgtcaactcg gcgctcgcga
     1201 agtccgaggc catgcgcgcc ggtttcgacg acgcgatcgt cctgacccag gacggccacg
     1261 tctcggaggg ctcggccgcc aacttcatgc tcgtccgcga cggagtcgtc atcacgccgc
     1321 cgatcaccga caacatcctc gaaggaatca cccgtcggac cgtgatggag cttctcgaca
     1381 aggagatggg ggtaacggtc ctcgaacgat ccatcgatcg aagcgagatc tacgtggcgg
     1441 atgaagcgtt cttcgtcgga acgggcgtgc agatcgttgc gatcacgcgg gtggaccacc
     1501 gtccgatcgg aaacggtcgc atcggtccga tcggcaccgc tttgagagac ctctacttcc
     1561 gcgtcgtgcg cggccggcac ccggcgtacc aggcgtggtg ctccggcgtc tatggggggg
     1621 ccgcggcgcg tgagaagacc gcgatgcgtc ccgccgagcc cgagcgcacg gccgccgccc
     1681 ggtagatccc cggacgcccc tccagcggtt cagtgaggga gggggatgta gccgagcggc
     1741 gcgcggagag agcggccgaa ctccgcggcc accattccct cccgggagag ctccggcacg
     1801 aaaaacccga aagcggggct cgagagccgc gccgtcgaaa ccgagtcgcc gcgcaggaac
     1861 ttcgcggtga tcttccgccc cttcggaagg ctgaagccga gcaggcagtg cgcgcccccg
     1921 tcgacgacga ggaggcgtcc gttcgaaaac cccgcgagga cttcgcgggc gttcgaggtg
     1981 ggcgtgtgcg cgtcgagcgt cccgctgacg aagagcaccg gcacggacga ggagatcggg
     2041 gcgcggaagt cctcgccgag atcgggaacg ccccacgcgt cgcagacggc cgggatcgga
     2101 aagtcggcag catcgccgag gagagccgac tgggcctgcg cggcaacccg ggctcgacga
     2161 gcggaggaac cccccgaggc gcaccgcatc gtgaagtcga ccgccggggg cgcggcgctc
     2221 ttgcgtcccg cgatcgagct cgccgcgagc tcctggaaat ctccccggag catcgcgagg
     2281 aagaggccgg ggaggcgcga gatctgttcg cgagacgaca gcatgttccg cgtgaagcac
     2341 tgaaggtcga actttccgag cgtgacggtg cggtgctctc ccgtcccggc gtccacgctc
     2401 tcgacgcgta tcggctcccg ctcgagcttc gacaggacgg tctggacgac gccgaggaag
     2461 ctcggcatgc gctcggccag gagtggagtg cgcttcacca tctcgtcgat ctgaacgagg
     2521 acttgctgaa agtcgccggg caatctctcc atgtggtccg gaccctgaac cccgaagagc
     2581 accgcgcgtt cgatcgagct ctcgaagagc cggatcgtcg cgagcgccag gtggcttccg
     2641 tagctcccgg cgaggagact cacccgcggc actccgagag cccgcctcag gtcctcgatg
     2701 tcgcgcgcgc tctcgaccgt gttgtac
//
LOCUS       DASWAV010000381         3907 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U2665
            k141_461089, whole genome shotgun sequence.
ACCESSION   DASWAV010000381 DASWAV010000000
VERSION     DASWAV010000381.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960316
            Sequence Read Archive: SRR10260007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 3907)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3907)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:47:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,929
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3907
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_461089"
                     /isolate="SMAG_U2665"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.36444 N 132.31889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            89..538
                     /locus_tag="VF958_07960"
     CDS             89..538
                     /locus_tag="VF958_07960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9149077.1"
                     /translation="MSRIRRPIVLSLAMFVISCALAFAGLMLHSETTIPIPVPGEKTS
                     LAKLEYRARFVASVDAVTLELASSDGGDPVIVEWTFVGSNTDGQVHRVAIALRLLDEA
                     GSQIGVSTGHGILSAGAKDQKISVSMKVKPDVWKAAKKVRIFADWMS"
     gene            568..2004
                     /gene="purF"
                     /locus_tag="VF958_07965"
     CDS             568..2004
                     /gene="purF"
                     /locus_tag="VF958_07965"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015744058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004044 - amidophosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HEX9149078.1"
                     /translation="MNRFSAALPSVPMQVRGGFHDECGIFGIADSEDAANIAYLGLYA
                     LQHRGQESAGIATLDGENVRVEREMGYVADVFDEIRISRLPGRTAIGHVRYSTAGASL
                     LANAQPIVFATGRGPLALAHNGNLVNAREIRRELEASGALFTTTSDSEVILHLVARSR
                     APDLTSALAEALLDVRGAYSILVLAREGIFAARDPNGIRPLSLGIREGSPVVASETCA
                     FDLIGARFERDVEPGEIVRLSREGFSSHRFAFSRSTPCIFEHVYFARPDSRVFGKSVA
                     ASREAFGARLAREHPAEADVVVPVPDSGLYAALGYARESGIPFGLGLVRNHYVGRTFI
                     EPKQAIRHFGVKVKLNPVREVVEGKRIVLVDDSIVRGTTSQKIVRMLREAGAREIHVR
                     VSSPPTVASCHYGMDTPTRRELIAANQSVDEIRAFIGADSLGYLSAEGMHEAFGRPPQ
                     SACAACFTGVYPVEVPEEELEKEGAVAR"
     gene            2009..3274
                     /locus_tag="VF958_07970"
     CDS             2009..3274
                     /locus_tag="VF958_07970"
                     /inference="COORDINATES: protein motif:HMM:NF014081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX9149079.1"
                     /translation="MIAAVIALRGATLVDGTGAAARPNALLVVENGRIVSLATASDEA
                     LARIPAGAEILDVAGKWIVPGLVDAHVHAESEADLKQMIRWGVTTARVMAEDVAAAGA
                     LAAASRGRRDVPEVFPAAPIFTAKGGWWDQGEPPDAHLDRFPATPAAARASVRKAKAL
                     GSREIKLMLDDMSWCRAPKPALPRIKADVARALISEARRRGLRAIVHAPELAEANEAI
                     ADGATALAHGVIEPLDDTTIAVMKTRPVFYIPTMDIFEFLGDTRAFVDSVLTDPLAAS
                     GVPAGTLEQYRSAAYSDRYRERYPNFENVKRRLPALRENLRKLHAAGVPIALGTDMWA
                     FPGLGVSIEMDLYVKSGFSPLEAIRSATETAARSIGVEKDRGTLEVGKRADFLVLSAD
                     PLADVKNVRAIDAVYRNGGRIDRGNVGGH"
     gene            3264..>3907
                     /locus_tag="VF958_07975"
     CDS             3264..>3907
                     /locus_tag="VF958_07975"
                     /inference="COORDINATES: protein motif:HMM:NF012488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HEX9149080.1"
                     /translation="MAIDELLRFRPEFPILEKTTYLISNSLGAMPRAAAAALAEYAET
                     WATRGVRAWAEGWWEMSAAVGDEIAPLIGASAGSVTMLPNVTIAAAVLLSSLDVRPPR
                     NRIVMVDGEFPTVRYLVETLGPRLGAEIVVVPSPGGDGLEADESRIRDAIDGRTALVL
                     LSHVLFKSAFVLDVAAIAEKCRQKGATLAVDGYQSVGTLRVAVEALGVDALVGGV"
BASE COUNT          567 a         1334 c         1364 g          642 t
ORIGIN      
        1 cctctcgcgg gtccacgaac gcagctcgcg cggctcgcga cgtcgctata atccggggaa
       61 ccccgatgcc tgacaaagga ggacttcgat gagccggatc agacgtccca tcgtgctctc
      121 cctggcgatg ttcgtaatct cctgcgccct cgccttcgcc gggctcatgc tgcactcgga
      181 gacgacgatc ccgattcccg ttcccggcga gaagacttcg ctcgcgaagc tcgagtaccg
      241 ggcgcgtttc gtcgcctcgg tcgacgcggt cacgctcgag ctcgcctcgt ccgacggcgg
      301 ggacccggtc atcgtcgaat ggacgttcgt cgggtcgaat accgacggcc aggtgcatcg
      361 cgtcgcgatc gcactgcgac tgctcgacga ggccggttcg cagatcggcg tctccacggg
      421 gcacggaatt ctctcggccg gggcgaagga ccagaagatt tcggtttcga tgaaagtgaa
      481 gccggacgtc tggaaggccg cgaagaaggt ccggatcttc gccgattgga tgtcctgacc
      541 ggccacggcg cttcggccgc gggaggcatg aaccggttct ccgcggcgtt accatcagtg
      601 ccgatgcagg tccggggcgg cttccacgac gagtgcggca tcttcgggat cgcggattcc
      661 gaggacgcgg caaacatcgc ctacctcggc ctctatgcgc tccagcaccg ggggcaggag
      721 tccgccggaa tcgcgacgct ggacggcgag aacgttcggg tcgagcgcga gatgggttac
      781 gtcgccgacg tcttcgacga gatccggatt tcgcggctcc cgggccggac cgcgatcggc
      841 cacgtgcgct actcgacggc gggggcgtcg ctcctcgcca acgcgcagcc gatcgtcttc
      901 gcgaccggcc gcggaccgct cgcgctcgcg cacaacggga acctcgtcaa cgcgcgcgag
      961 atccgcaggg agctggaggc ctcgggcgct ctcttcacga cgacttccga ttcggaagtc
     1021 atcctgcacc tcgtggcgcg ctcccgggcg ccggatctca cgtccgccct cgcggaggcg
     1081 ctgctcgacg ttcgcggcgc gtactcgatc ctcgtgctgg cccgcgaggg catcttcgcc
     1141 gcccgcgacc ccaacggcat ccggccgctt tcgctcggaa tccgcgaggg atcgccggtc
     1201 gtcgcgtcgg agacgtgtgc gttcgacctc atcggcgccc gtttcgagcg cgacgtcgag
     1261 ccgggcgaga tcgtccgcct gtcgcgggag gggttctctt cgcaccgctt cgccttctcc
     1321 cgctcgacgc cctgcatctt cgagcacgtg tacttcgcgc gtccggactc cagggtcttc
     1381 ggcaagagcg tcgccgcctc gcgcgaggcg ttcggcgcgc ggctcgcccg cgagcatccg
     1441 gccgaagcgg acgtcgtcgt gcccgtcccc gactcgggac tgtacgcggc gctcggttac
     1501 gctcgcgagt ccggaatccc cttcggactg gggctcgtga ggaaccacta cgtcgggcgc
     1561 acgttcatcg agccgaagca ggcgatccgg catttcggcg tcaaggtgaa gctgaacccg
     1621 gtccgggagg tcgtcgaagg caagcggatc gtgctcgtgg acgactcgat cgtccggggc
     1681 acgacttctc agaagatcgt ccgtatgctg cgcgaagcgg gcgcccggga gatccacgtg
     1741 cgggtctctt cgcctccgac ggtcgcgtcc tgtcactacg gcatggatac gccgacacgg
     1801 cgcgagctca tcgcggcgaa ccagtcggtc gacgagatcc gcgcgttcat cggggcggac
     1861 agcctcggct acctctccgc ggagggcatg cacgaggcgt tcggtcggcc gccccagtcg
     1921 gcctgcgccg catgtttcac cggggtctat cccgtcgagg tcccggaaga ggagctcgaa
     1981 aaagagggag ccgtcgcccg ttaggacgtt gatcgccgcg gtcatcgcct tgcgcggcgc
     2041 gacgctcgtc gacggcacgg gcgcggccgc tcgcccgaac gctcttctcg tggtcgagaa
     2101 cggccggatc gtctccctcg cgacggcgtc ggacgaagcc ctcgcccgga ttccggcggg
     2161 cgccgagatc ctcgacgtcg cggggaagtg gatcgttccg ggcctcgtcg acgcgcacgt
     2221 ccatgcggaa tccgaggcgg acctcaagca gatgattcgc tggggggtga cgaccgcgcg
     2281 cgtcatggct gaggacgtcg ccgccgccgg cgcgctcgcg gcggcctcgc gcgggaggcg
     2341 ggacgttccg gaggtcttcc cggccgcgcc gatcttcacg gcgaaaggcg gttggtggga
     2401 ccagggcgag ccgccggacg ctcacctcga ccgtttcccc gcgacgcccg cagccgcgcg
     2461 cgcctccgtg cggaaggcga aggcgctcgg gtcgcgcgag atcaagctga tgcttgacga
     2521 catgtcgtgg tgccgagctc cgaaaccggc gcttccgaga atcaaggccg acgtcgcgag
     2581 ggcgctcatc tcggaggccc gacgccgggg tctgcgcgcg atcgtccacg ctccggagct
     2641 cgccgaagcg aacgaggcga tcgccgacgg cgcgacggcg ctcgcgcacg gcgtgatcga
     2701 gccgctcgac gacaccacga tcgccgtgat gaagacccgg cccgtcttct acattccgac
     2761 catggacatc ttcgagttcc tcggggacac gcgggcgttc gtcgattcgg tcctcaccga
     2821 tccgctcgcc gcctccgggg tgccggcggg cacgctcgaa cagtaccggt cggcggcgta
     2881 ctcggaccgt taccgcgagc gttaccccaa cttcgagaac gtgaagcgcc gccttccggc
     2941 gctccgcgag aatctgcgga agctccacgc cgctggggtc ccgatcgcgc tcggcacgga
     3001 catgtgggcg tttcccgggc tgggcgtctc gatcgagatg gacctctacg tcaaatcggg
     3061 attctctccc ctggaggcga tccggtcggc gacggagacg gcggcgcgct cgatcggagt
     3121 cgagaaggac cgcggcacgc tcgaggtggg aaagcgggca gactttctcg tgctctcggc
     3181 agacccgctt gccgacgtga agaacgtccg tgcgatcgat gcggtttacc ggaacggcgg
     3241 gcggatcgac cgggggaacg tcggtggcca ttgacgagct cctgcgcttt cgtccggagt
     3301 tcccgattct cgagaagacg acgtacctga tctccaactc cttgggcgcc atgccccggg
     3361 ccgccgcggc cgcgctcgcc gagtacgccg agacctgggc gacgcgcggc gttcgggcgt
     3421 gggcggaggg ctggtgggag atgtcggcag cggtcggcga cgagatcgct ccgctcatcg
     3481 gcgcgtccgc cggctcggtg acgatgctgc cgaacgtcac gatcgcggcg gcggtcctgc
     3541 tttcgtcttt ggacgtccgg ccgccgcgca accgcatcgt catggtcgac ggggagttcc
     3601 cgacggtccg ctacctcgtg gagacgctcg ggccgcgcct gggtgcggaa atcgtcgtgg
     3661 tgccgtcgcc gggaggagac ggactcgagg ccgacgagtc gcgaatccgc gacgcgatcg
     3721 acggccggac cgctctcgtc ctcctttcgc acgttctctt caagagcgca ttcgtgctcg
     3781 acgtcgccgc gatcgcggag aagtgccgcc agaaaggcgc gacgctcgcc gtcgacggct
     3841 accagtcggt gggcacgctt cgggtcgccg tggaggcgct cggggtcgac gctctggtgg
     3901 gcggcgt
//
LOCUS       DASWAV010000382         1965 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U2665
            k141_621821, whole genome shotgun sequence.
ACCESSION   DASWAV010000382 DASWAV010000000
VERSION     DASWAV010000382.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960316
            Sequence Read Archive: SRR10260007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 1965)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1965)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:47:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,929
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1965
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_621821"
                     /isolate="SMAG_U2665"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.36444 N 132.31889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1306
                     /locus_tag="VF958_07980"
     CDS             <1..1306
                     /locus_tag="VF958_07980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9149081.1"
                     /translation="RLSKDGGSTWTLTGDTTPGLYQVESLAFAPDDPDTLYAGTWRQA
                     FRTRDGGRTWSRIAEGMVLDATVYAWDFSPSDPKDIWVSTCGWVYRTQNGGDRWTRFK
                     SGFTNRRSHNVRRDPKRPEIIYAGTVGGLHRSTDNGETWSRISRETLVVTALDVDRRN
                     GRLYIGTEGEGVFYSDDRGVTLLPGSVGLAEGRVSDLVADPRDHSRLFFFRAYAGGES
                     GVWEAKGKRVRRLSLDPLPPAASLAAFVDPAGDTVLLLSSFNGVRVSFDGGQRWSVPE
                     KAPPGTPLALFGKEFGSPVVVTTGGVFRTPDGRNFVPVAGTPHAPSSVELLADGEGSP
                     ILEVKTVTGAFRWDGVAWDTRRRTLLAGGRFLDAYRGEIGPLPVRTPVREVDGNLVWE
                     ESGRRRAVKSPRPGLSVASTFATPEGRLYVGTAGDGLFLFEP"
     gene            complement(1395..>1965)
                     /locus_tag="VF958_07985"
     CDS             complement(1395..>1965)
                     /locus_tag="VF958_07985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015185447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF192 domain-containing protein"
                     /protein_id="HEX9149082.1"
                     /translation="ASSGATTFTKGRHRPTARILSPPKGHKLRSSMRGAHSLVLVLAL
                     TVSCGGAAKPAPSPTPSPAAVGSPQVVLPSGAIYRIELARTPEEQQRGLMFRESLPER
                     SGMLFLFDDDSPHRFWMKNTMIPLDIVWLDDVGRVLFVSANTPPCKADPCPDYGPQVA
                     AWNVLEIAGGMAEKERVTVGSTLRFEGIK"
BASE COUNT          306 a          690 c          655 g          314 t
ORIGIN      
        1 gcggctctcg aaggacggcg gcagcacctg gaccttgacc ggcgacacga ctcccggcct
       61 ctaccaggtc gagtcgctcg cgttcgcgcc ggacgacccc gataccctct acgccggcac
      121 gtggcggcag gcgttccgca cccgggacgg cgggcgcacg tggagtcgca tcgcggaagg
      181 catggtgctc gacgccacgg tctacgcctg ggacttctcc ccgtccgatc ccaaggacat
      241 ctgggtctcg acgtgcggct gggtctatcg gacgcagaac ggcggcgacc gctggacgcg
      301 cttcaagagc ggcttcacga accggcgctc gcacaacgtg cgccgcgatc ccaaacggcc
      361 ggaaatcatc tacgccggaa ccgtcggcgg cttgcaccgc tcgaccgaca acggcgagac
      421 ctggtcgcgc atctcccgcg agacgctcgt cgtgaccgcg ctcgacgtcg atcggcgcaa
      481 cggacgcctc tatatcggga ccgaaggaga aggagtcttc tactccgacg accgcggggt
      541 cacgcttctg ccggggtccg tgggtctcgc ggaggggcgc gtctccgacc tcgtcgcgga
      601 tccccgggat cacagccggc tcttcttctt ccgcgcgtac gccggcggcg agagcggggt
      661 gtgggaagcg aagggcaagc gcgtgcgccg cctctcgctc gacccgctgc cccccgcggc
      721 atccctggcg gcgttcgtcg atccggccgg ggacaccgtc ctcttgctct cgagcttcaa
      781 cggagtgcgg gtttcgttcg acggcggcca gcgctggtcc gttccggaga aggctccgcc
      841 cgggaccccc ctcgcgctct tcggaaaaga attcggctcg cccgtcgtgg tgacgaccgg
      901 cggcgtgttc cggacgccgg atgggcgcaa cttcgttccg gtcgccggga ccccgcacgc
      961 gccgtcttcc gtcgagctgc tcgccgacgg cgaaggctcg ccgatcctcg aggtgaagac
     1021 cgttacggga gcgtttcgat gggacggcgt cgcctgggat acgcgacgcc ggacgctgct
     1081 cgcggggggc cgcttcctgg acgcgtaccg cggggagatc ggccctctcc ccgtgcgcac
     1141 gcccgtgcga gaagtcgacg gcaatcttgt gtgggaggaa tccggacggc gacgcgcggt
     1201 caagagtcca cgccccggcc tttcggtcgc gtcgaccttc gcgacgcccg aaggacgact
     1261 ctacgtcgga accgcaggtg acggtctctt cttgttcgaa ccctgacgct gcggagcgag
     1321 aagcgggaca cccgcacgaa ctccatctcc ctcccgggag agaggcgggg tgagtgagct
     1381 gactccgctt cgctctactt gatcccctcg aaccggagcg tggatccgac ggtcaccctc
     1441 tccttctccg ccatgcctcc cgcgatctcg agcacattcc acgcggccac ctgcggcccg
     1501 tagtccggac acggatccgc cttgcagggc ggagtattcg ccgagacgaa gagcaccctc
     1561 ccgacgtcgt ccagccagac gatgtcgagc gggatcatcg tgttcttcat ccagaatctg
     1621 tgcggagagt cgtcgtcgaa gaggaacaac atgcccgacc gctcgggaag gctctcgcga
     1681 aacatcagcc cgcgctgctg ctcttcggga gtgcgcgcga gctcgatccg gtagatcgcc
     1741 cccgacggaa gcacgacctg cgggctgccc accgccgccg gcgatggggt cggagatggc
     1801 gccggctttg ccgccccccc gcacgagacg gtgagcgcga gaacgagaac gagagagtga
     1861 gctccccgca tcgacgaacg gagcttatgc cccttcggcg gtgagaggat tctcgccgtt
     1921 ggccgatgcc tccccttcgt aaatgtcgtc gcgccggacg aggct
//
LOCUS       DASWAV010000383         4759 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U2665
            k141_621833, whole genome shotgun sequence.
ACCESSION   DASWAV010000383 DASWAV010000000
VERSION     DASWAV010000383.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960316
            Sequence Read Archive: SRR10260007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 4759)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4759)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:47:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,929
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4759
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_621833"
                     /isolate="SMAG_U2665"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.36444 N 132.31889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1468
                     /locus_tag="VF958_07990"
     CDS             <1..1468
                     /locus_tag="VF958_07990"
                     /inference="COORDINATES: protein motif:HMM:TIGR00757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="Rne/Rng family ribonuclease"
                     /protein_id="HEX9149083.1"
                     /translation="VNATPPETRVAILEDGRTVEVLHERRGRQGLVGNIYLGRVHRVL
                     PGMHAAFVSIGLDRDAFLYVEDVLARSADLDSDGDEEGPRDGGREAADRPRIDDLLKE
                     GQELVVQVTKDALGGKGPRVTANLSLPGRTLVYLPGVREVGISRRITNEEERERLRRI
                     LEGLPGDGGLIARTVAAQSSGAEFLAEHQYLTQLAARISRRAETSSAPAILHRELDLA
                     VRAARDLVAGDFAAIRVDEEETYARLAEFLGQVAPSLAYRLELDRGPEPLFERFGVEA
                     EIESALKSRVPLASGGSIVIHQTEALVAIDVNTGKYVGKEDLEETVFETNLEAVPEVA
                     RQIRLRDLGGLLVVDFIDMLDEAHRREVSERFEAELAKDRARFRILPVSEFGLIEITR
                     QRSRGNLEKLLTRSCPDCGGSGRVKTHLTVALELRRALLSPQVLSVAGATVRVRVRPA
                     LAKFLAEEEPTLLADVGIDVELVADATLPPAHYEINGK"
     gene            complement(1596..3221)
                     /locus_tag="VF958_07995"
     CDS             complement(1596..3221)
                     /locus_tag="VF958_07995"
                     /EC_number="6.5.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA ligase"
                     /protein_id="HEX9149084.1"
                     /translation="MTDYRVLAWTLDAVAKERGKLAKIERFTETLDRLEGEELKVAAR
                     LASGSPFAEWEQAVTSVGWVTVARASSEVTGWDLETIGACAAAIGDLGEAVGLLLTDE
                     PADPMTILEVDARLRELARERKAAGKQAAVAAMLRRSSPVEGKYLVKTLSGGLRVGAD
                     VTTVEEAIAQAFGADREAVARAHRDSGDIGETAVAARDGKLSEILFRLFHPIGFMLAT
                     PIETAGEVAEELRLFAIEDKYDGIRAHAHKSDNRVALFSRTLDDVTPQFPEIAESLAK
                     LSGSFLLDGEILAWRENRPDSFFKLQRRLGRKAPAPELRSEIPVAFVAYDCLARDGRP
                     LFEEEWSARRRELEAISPELLVSPVHRAETPGELDSLFDAARERGNEGLMLKRRDSTY
                     QAGKRGRAWRKYKKALATLDVVVTAVQQGNGKRAGMLSDYTFAVRDGDRLVNIGKAYS
                     GLTDEEIRKMGAHFRKITVARYGPVRAVEPQVVLEVSFDSIQKSTRHKSGFALRFPRI
                     VRLRPDKRPEEADTLQTVAELYGRITRRERSAT"
     gene            complement(3218..4252)
                     /locus_tag="VF958_08000"
     CDS             complement(3218..4252)
                     /locus_tag="VF958_08000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9149085.1"
                     /translation="MNDLLAPLLELRPEGLHCPALDAAVDPPLPARRAILSHAHSDHA
                     APGHEEIWGTPETIAVYGRRNPDWAGEAVEMHYGERFERHGVTVELYPAGHVLGSAQV
                     FFEREGRSLLYTGDFKRRESRTSVPAAAPPAEILATETTFGLPVFRFPKREVLEDRLV
                     AACHSAFAEDRTPILLAYGFGKAQEVALALTDAGIPTVVHGAAWKLVPEFEAAGHAFP
                     TSRAYETGHAQPGEALVVPPSCARTAVVRNVKRRRIIYLSGWAMREASRADFDADVLI
                     PWSDHADFPELLQHVADVAPRRVVTLHGYSRDFARILMNRGVAAEPLPSARERLSEDP
                     AAGGGEENGA"
     gene            complement(4249..>4759)
                     /locus_tag="VF958_08005"
     CDS             complement(4249..>4759)
                     /locus_tag="VF958_08005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014235702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="FAD-binding domain-containing protein"
                     /protein_id="HEX9149086.1"
                     /translation="DGEAIAAWQEGRTGYPFVDAGMRQLRETHWMHNRARMTVASFLT
                     KDLHADWKVGEKWFEHELADADLANNNGGWQWAAGTGADAAPYFRILNPVLQSKRFDP
                     DGTYIRTYVPELARVPGSKVHEPWTMAPEEQKASGCVIGRDYPAPIVDHARERVTALA
                     LFEKIRGSR"
BASE COUNT          706 a         1640 c         1639 g          774 t
ORIGIN      
        1 ggtcaacgcc acgcctccgg agacgcgggt cgcgatcctg gaggacggtc gcaccgtgga
       61 agtcctgcac gagcgccggg gccgccaggg tctcgtcggc aacatctacc tcggccgtgt
      121 ccaccgtgtg ctgcccggga tgcacgccgc gttcgtctcg atcggcctcg accgcgacgc
      181 gttcctctac gtcgaggacg tgctcgcccg gtcggccgat ctcgactccg atggggacga
      241 ggagggtccc cgcgacgggg ggcgcgaggc cgccgatcgg ccgaggatcg acgacctgct
      301 gaaggagggc caggagctcg tcgtccaggt aaccaaggat gctctcggcg ggaaaggccc
      361 gcgcgtgacg gcgaacctct cgctgcccgg acgcactctc gtctacctgc cgggcgtccg
      421 ggaggtcggg atctcgcgcc gcatcaccaa cgaggaggag cgcgagcgcc tccgtcgcat
      481 tctggaaggt cttccgggcg acggtggcct gatcgctcga acggttgcgg cgcagagctc
      541 gggcgccgaa ttcctcgccg agcaccagta tctgacgcaa ctcgccgcca ggatttcgcg
      601 cagggcggag acttcgtccg cacccgcgat tctgcaccgg gaactcgacc tcgccgtccg
      661 ggcggcgcgg gatctggtcg ccggagactt cgcggcgatt cgggtcgacg aggaggagac
      721 gtacgcccgg ctcgccgaat tcctcgggca ggtggcgcca tcgcttgcct atcgtctgga
      781 gctcgatcgc ggccccgaac cgctgttcga gaggttcggc gtggaggcgg aaatcgagag
      841 cgcgctcaag agccgtgtgc cgctggcgtc ggggggctcg atcgtcatcc atcagaccga
      901 agctctcgtc gcgatcgacg tcaacacggg caagtacgtg gggaaggagg acctcgaaga
      961 gaccgttttc gagacgaacc tcgaggcggt cccggaagtc gcgaggcaga tccgtctgcg
     1021 tgatctcggc ggcctcctcg tcgtcgactt catcgacatg ctggatgagg cgcaccgccg
     1081 cgaggtctcg gagcgattcg aagcggagct cgcgaaggac cgcgcgaggt ttcggattct
     1141 gccggtttcg gagttcgggt tgatcgagat cacccgacaa cgctcgcgcg ggaacctgga
     1201 gaaactcctg acccgctcct gccccgactg cggcggcagc ggacgggtca agacgcactt
     1261 gaccgtcgcc ctcgagctgc gccgcgcgct tctctccccg caggtcctct cggtggcggg
     1321 cgccaccgtc cgggtgcgcg tccgtcccgc cctggcgaag ttcctcgcgg aggaggagcc
     1381 cacgctcctc gccgacgtcg ggatcgatgt tgagctcgtc gccgacgcga cgcttcctcc
     1441 ggcgcactac gaaataaacg gcaagtagtt ttctttcaaa tagttgcgag ccggaatagt
     1501 ccggccctcg cccgcgttgg caggatcagc acgcgatacc ttccttcccg gccccgacca
     1561 tttgagtcgg ggccgccttt tttaggggcc gaagctcaag tcgcggagcg ctcccttctt
     1621 gtgatccggc cgtacagctc cgcgacggtc tggagcgtat cggcctcctc gggccgcttg
     1681 tccggccgga gccgcacgat ccgcgggaag cggagcgcga acccggactt gtgacgcgtg
     1741 ctcttctgga tcgaatcgaa tgaaacttcc agcacgacct gcggttcgac cgcccgcacc
     1801 gggccgtacc gcgccaccgt gatcttgcgg aagtgcgctc ccatcttccg gatctcctcg
     1861 tcggtcagtc ccgagtatgc ctttccgatg ttgacgagcc ggtcgccgtc gcgcacggcg
     1921 aacgtgtagt ccgacagcat cccggctcgc ttgccgttgc cctgctggac ggcggtgacg
     1981 acgacgtcga gcgtggccag cgccttcttg tactttcgcc acgcgcggcc gcgcttgccc
     2041 gcctgatacg tcgagtcccg gcgtttcagc atcagccctt cgttgccgcg ctcgcgcgcc
     2101 gcgtcgaaga gcgagtcgag ctccccgggc gtttccgccc gatggacggg tgacacgagg
     2161 agctcgggcg agatcgcctc gagttcgcgt cgtctcgcgc tccattcctc ttcgaagagc
     2221 gggcggccgt cccgcgcgag acagtcgtag gccacaaacg ccacgggaat ctcggaccgg
     2281 agctcgggcg ccggagcttt gcggcccagc cggcgctgca gcttgaaaaa ggagtcgggg
     2341 cgattctcgc gccacgcgag aatctccccg tcgaggagaa acgacccgga aagcttcgcg
     2401 agcgattccg cgatttccgg aaactgcggg gtgacgtcgt cgagggttcg cgagaagagc
     2461 gcgactcggt tgtccgactt gtgagcgtgc gcgcgaatgc cgtcgtactt gtcctcgatc
     2521 gcaaagagcc tgagctcttc ggcgacttcg ccggccgtct cgatcggggt cgccagcatg
     2581 aagccgatcg gatggaagag acggaagagg atctcggaga gcttcccatc ccgggccgcg
     2641 accgccgtct ctccgatgtc gcccgaatcc cggtgcgcgc gcgcgaccgc ctcgcggtcc
     2701 gcgccgaacg cctgggcgat cgcctcctcg acggtcgtca cgtcggcgcc gacgcgcagg
     2761 ccaccggaga gcgtcttgac gaggtacttc ccctcgacgg gagaggagcg ccggagcatc
     2821 gccgcgaccg ccgcctgctt cccggcggcc ttgcgttcgc gggcgagctc acgcagtcgt
     2881 gcgtcgacct cgagaatcgt catcgggtcg gccggctcgt cggtcaggag caatccgacc
     2941 gcctcgccga ggtcgccgat cgcggcggcg caggcgccga tggtttccaa gtcccaaccc
     3001 gtgacttcgg aggaggcgcg cgcgacggtg acccatccga ccgacgtgac cgcctgctcc
     3061 cactcggcga aagggctgcc ggacgcgagc cgggccgcga ccttcagctc ctccccctcg
     3121 aggcggtcga gggtctccgt gaacctctcg atcttcgcga gcttgccgcg ctccttcgcg
     3181 accgcgtcga gggtccaggc gaggacgcgg taatcggtca cgcgccgttc tcctctccac
     3241 cgccggcggc gggatcctcc gacaggcgtt cgcgcgccga cgggagcggc tccgcggcaa
     3301 cgccccgatt catcaggatg cgcgcgaagt cgcgcgagta accgtgcagg gtgacgactc
     3361 ggcgcggcgc cacgtcggcg acgtgctgga ggagctccgg gaaatccgcg tggtcggacc
     3421 agggaatgag gacgtcggcg tcgaagtccg ctcgggacgc ctcgcgcatc gcccatcccg
     3481 acaggtagat gatccgccgc cgcttcacgt tgcggacgac cgccgttcgc gcgcagctcg
     3541 gcggcacgac gagcgcctcg ccgggctgcg cgtggccggt ctcgtaggcg cgcgaggtcg
     3601 ggaacgcgtg cccggccgct tcgaactccg gcacgagctt ccaggctgcg ccgtggacga
     3661 ccgtcgggat gccggcgtcc gtcagcgcga gcgcgacctc ctgcgccttg ccaaacccgt
     3721 acgcgagcag gatgggcgtc ctatcttccg cgaaggccga gtggcacgcg gcgacgaggc
     3781 ggtcctcgag aacctccctt ttcgggaagc ggaacacggg aagcccgaac gtcgtctcag
     3841 tggcgaggat ctcggcggga ggcgccgcgg ccggcacgga cgttcgcgac tcgcggcgct
     3901 tgaagtcgcc ggtgtagaga agagatcgcc cctcccgctc gaaaaagacc tgcgccgagc
     3961 cgaggacgtg gcccgcgggg tagagctcga ccgtcacgcc gtgccgctcg aagcgctcgc
     4021 cgtagtgcat ctcgaccgcc tcgcccgccc agtccgggtt tcgtcgcccg tagaccgcga
     4081 tcgtttcggg agtgccccag atctcttcgt ggcccggcgc ggcgtggtcg ctgtgggcgt
     4141 gggaaaggat cgcgcggcgc gccgggagcg gcgggtcgac ggccgcgtcg agtgcgggac
     4201 agtggaggcc ctcgggccgg agctcgagca ggggcgcgag caggtcgttc accgcgaccc
     4261 tcgaatcttt tcgaacagcg cgagcgcggt cacccgctcc cgggcgtgat cgacgatcgg
     4321 cgccgggtag tcgcgtccga tgacgcagcc cgacgccttc tgctcttccg gcgccatggt
     4381 ccacggctcg tgaacttttg accccgggac gcgcgcgagc tccggcacgt aggtccggat
     4441 gtacgtgccg tccggatcga agcgcttcga ctggagcacg gggttcagga tacggaagta
     4501 cggggcggcg tcggcgccgg tccccgcggc ccactgccag ccgccgttgt tgttcgccag
     4561 gtccgcgtca gcgagctcgt gctcgaacca cttctcgccc accttccagt cggcgtgcag
     4621 gtccttcgtc aggaacgagg cgactgtcat ccgggcgcgg ttgtgcatcc agtgggtctc
     4681 ccgaagctgc cgcatgcccg cgtccacgaa cggatacccg gtgcgacctt cctgccaggc
     4741 cgcgatcgct tctccgtcg
//
LOCUS       DASWAV010000384         2596 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U2665
            k141_421587, whole genome shotgun sequence.
ACCESSION   DASWAV010000384 DASWAV010000000
VERSION     DASWAV010000384.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960316
            Sequence Read Archive: SRR10260007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2596)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2596)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:47:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,929
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2596
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_421587"
                     /isolate="SMAG_U2665"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.36444 N 132.31889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..450
                     /locus_tag="VF958_08010"
     CDS             <1..450
                     /locus_tag="VF958_08010"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006979737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HEX9149087.1"
                     /translation="RLVITRPALREMSHGRWEQKTRAEVEDLYPREYDRWESDPFSFA
                     PEGGETGLAVTARALPALLEIVAVHTDGQVLVVSHKATIRLLISALLGFDARKYRDRL
                     DQSPGCLNILDFRDLTRARLTLFNDTSHYGTRTPAIPVDRLSKWWSS"
     gene            complement(464..2329)
                     /locus_tag="VF958_08015"
     CDS             complement(464..2329)
                     /locus_tag="VF958_08015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014100521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HEX9149088.1"
                     /translation="MKQAIYGGVSKAAGVLWRAARAFDRRFPQRSFHPRWAPAPLPKQ
                     HERTFPQLGFPRQTDSLCPRCVSEVRAEILSGEADWKILVDGKPGEIKASIVEEDGKI
                     LMRKDCPKHGRFEDVMSVDPDFFKRIESLYPGRDFVIPKDGLHEHGTSSIKYGRGAVL
                     TIDLTNRCNMMCNPCFMDANQVGYVHELEWEDVKKMLDDAASVKPRRQLSVQFSGGEP
                     TLSPHFLPAIRYAREVGYFSVQCATNGIRFAQDETFARDAAEVGLRFVYLQFDGVGNE
                     HNKHRKVGNLYDVKLRAIENLHKYGVDITLVTTIVNGVNNDQVGDILKFAIENIDKIN
                     TVSFQPVSFTGRDEEIDDETRQKQRYTLSHLAHDAKAQAGIGEPLRDWYPLSASGPFS
                     DLKDLLGGLEAEWGSLKCGCHPNCGIGTMLLVHQETRRAVTVPSILNIDQLLQDVKTI
                     TDSGRKKFWTAAQVAISVLRNLRPGGIPTGLSPLSVIKIMDGHTGGKLGVAKAKRYDW
                     RVFFIGGMWFQDLFNYDFRRTEMCIIPYATQMGEISFCAYNTGVGWRKIIEEMFQTAS
                     LADWYKTRGRHEVFARGRSVPLPAFPGQPLPATQPARPAATGGGVALPVYGGGAR"
BASE COUNT          443 a          838 c          830 g          485 t
ORIGIN      
        1 cggctcgtga tcacgcgccc cgctctacgg gaaatgtcgc atgggcgttg ggagcagaag
       61 acccgggcgg aagtggagga cctctacccc cgagaatacg accgctggga gtccgaccct
      121 ttctctttcg ctccggaagg cggtgagacc ggcctcgccg tgaccgcgcg cgctcttccg
      181 gcgcttctcg aaatcgtcgc ggtgcacacg gacggccaag tcctggtcgt gtcgcacaag
      241 gcgacgatcc ggctgcttat cagcgcactt ctcgggttcg acgcgcgaaa gtatcgcgat
      301 cgtctcgacc agagtcccgg ctgcctgaac atcctggatt tccgggacct gacccgagcg
      361 cggcttactc tcttcaacga cacgtcgcat tacggaacac gcacaccggc gattccggta
      421 gatcgcctgt ccaaatggtg gagttcttag cggatcgggg cgactacctt gctccgccgc
      481 cgtaaaccgg cagcgcgaca ccgcccccgg ttgcggccgg gcgcgcgggt tgcgtcgccg
      541 gcaacggctg tccggggaac gcgggcaaag gcacgctgcg gccccgcgcg aacacctcat
      601 ggcggccgcg cgtcttgtac cagtccgcaa gcgacgcggt ctggaacatc tcctcgatga
      661 tcttgcgcca gcccacgccg gtgttgtacg cgcagaacga gatctctccc atctgggtcg
      721 cgtacgggat aatgcacatc tccgtgcggc ggaagtcgta gttgaacaga tcctggaacc
      781 acattccgcc gatgaagaag acgcgccagt cgtagcgctt cgccttcgcg acgccgagct
      841 tgccgccggt gtggccgtcc atgatcttga tcaccgacag cggtgagagc ccggtcggga
      901 tgcctccggg ccgaagattc cgcaggaccg agatcgcgac ctgcgccgcg gtccagaact
      961 tcttccgtcc cgagtccgtg atcgtcttga cgtcctggag aagctgatcg atgttcagga
     1021 tcgacggcac cgtcaccgcc cggcgcgtct cctggtggac gagcagcatc gtgccgatcc
     1081 cgcagttggg gtgacagccg cacttcaacg acccccactc cgcctcgagg ccgccgagca
     1141 ggtccttgag gtcggagaac ggacccgacg ccgaaagcgg gtaccagtcg cgcagcggct
     1201 cgccgattcc ggcctgcgcc ttggcgtcgt gcgcgaggtg cgacagcgtg taacgctgct
     1261 tctggcgcgt ctcgtcgtcg atttcctcgt ctcttcccgt aaacgagacg ggctggaacg
     1321 agaccgtgtt gatcttgtcg atgttctcga tcgcaaattt caagatgtcg ccgacctgat
     1381 cgttgttgac tccgttcacg atcgtggtga cgagcgtgat gtcgacgccg tacttgtgca
     1441 ggttctcgat cgcgcgcaac ttgacgtcgt agaggttgcc gaccttgcgg tgcttgttgt
     1501 gctcattgcc gacgccgtcg aactggaggt acacgaagcg cagcccgacc tccgcagcgt
     1561 cccgggcgaa ggtctcgtcc tgcgcaaaac gaatcccgtt cgtcgcgcac tggaccgaga
     1621 aatacccgac ctcccgcgcg tatcggatcg ccgggagaaa gtgcggcgag agcgtcggtt
     1681 cgccgccgga gaactggacc gacagctgcc gtcgcggctt gaccgaggcc gcgtcgtcca
     1741 gcattttctt gacgtcttcc cactcgagct cgtggacgta tccgacctgg ttcgcgtcca
     1801 tgaagcacgg gttgcacatc atgttgcagc ggttcgtgag gtcgatcgtg aggactgcgc
     1861 ctcgcccgta cttgatcgac gacgttccgt gctcgtggag gccgtccttc gggatgacga
     1921 agtcgcggcc gggatacagg gactcgattc gcttgaaaaa gtcgggatcg acggacatga
     1981 cgtcctcgaa gcggccgtgc ttcgggcagt ccttgcgcat gaggatcttg ccgtcttctt
     2041 cgacgatcga ggccttgatc tctccgggct tcccgtcgac gagaatcttc cagtccgcct
     2101 cgcccgacag gatctcggcc cggacctccg agacgcagcg gggacagagc gaatcggtct
     2161 gccgtggaaa gccgagctgc gggaaggttc gctcgtgctg cttcgggagc ggagccgggg
     2221 cccagcgcgg gtgaaaggaa cgttgcggga accgccggtc gaaggcacgc gcggcgcgcc
     2281 agagaacgcc tgcggctttc gacacccctc cgtagatcgc ctgtttcata gtgattccca
     2341 cttgaggcgg cgtcgagacc gcctcccctc cgttttttgc cgctccccct tccggaaaaa
     2401 atccggaacg gttgctcgcg attctaaccg aaataccgcg gtcatcggtc gcgggcggcg
     2461 gcgaatctct ccggcgcgaa gggcgcgagg tcgtgcaccg cgacaccgct gagaaggtcc
     2521 gcgattcgaa gcgccgtgat cggcgcgagg agaatcccgt atcggaagtg ccccgtcgcg
     2581 aggtagagcc cggcga
//
LOCUS       DASWAV010000385         2372 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U2665
            k141_101014, whole genome shotgun sequence.
ACCESSION   DASWAV010000385 DASWAV010000000
VERSION     DASWAV010000385.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960316
            Sequence Read Archive: SRR10260007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2372)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2372)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:47:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,929
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2372
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_101014"
                     /isolate="SMAG_U2665"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.36444 N 132.31889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1176)
                     /locus_tag="VF958_08020"
     CDS             complement(<1..1176)
                     /locus_tag="VF958_08020"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HEX9149089.1"
                     /translation="MKVLVVDPDPESRDALRLAFAARGCRARCVASAAEGRRYLEELL
                     PDVVVAAADLSDSVGESFLAEAAGDPRRAVWALVPRERIEVSVEAMERGAGDFLWRPV
                     SDRRLALLLERLRRRRRTEGDAEESRLRLARVESAVTLPGRSPRWTAAMAAIERLAAE
                     NAVLVTGEEGTEKEAAARFLHRLSARGAEPFVKIAAGERFADALLRAGGGTVYLAGVD
                     RAPLAIQAEILSALEREERPGLILSADRDPVEAVRRGHLEDEIVRRLAERVLHLPPLR
                     ERGEDVARLARHFLEETSATLSFDVEAMDALAAHDWPGNVQELREAVKRAAKLAEGPV
                     IGPTVVRSVLGRPLAARGSRRRRVPVVRITVGDSLADVERRLIQKTLEFARGNKRKTA
                     "
     gene            complement(1204..>2372)
                     /locus_tag="VF958_08025"
     CDS             complement(1204..>2372)
                     /locus_tag="VF958_08025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013907652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="menaquinone biosynthesis decarboxylase"
                     /protein_id="HEX9149090.1"
                     /translation="LIEKLKTIPKVTELAQIFPKTVRSGPCQEIVETGEEVDLGKLPI
                     LTCWPQDAGPFITLPLVITRDPSSGKVNVGVYRMQVFDRNTTGMHWQKHKDGAGQARG
                     YEREGRRMEVAVAIGADPATVFSGIAPLPPGISEFLFAGFLRGESVPLVAAKTVDVLV
                     PAEAEIVLEGYVDPGERRREGPFGDHTGFYSLDDDFPVFHVTAVTRRREPVYLTTIVG
                     KPPMEDGFMGEAVERLFLPLVRRMIPEIIDMHMPVHGIFHNLMLVSIDKRYPGHARKT
                     MHAIWGTGQMMFTKTIVVFDRDADLRNGPDALWRALTAIDPERDIELVHGPVDELDFA
                     SRLPCYGSKIGIDATRKWKEEGFSRRWPDEISMSEEVRRRVDAAWPELGIRLRR"
BASE COUNT          382 a          828 c          782 g          380 t
ORIGIN      
        1 cgcggtcttt cgcttgttgc cgcgcgcgaa ctcgagcgtc ttctggatca ggcggcgttc
       61 cacgtcggcg agcgagtcac cgacggtgat ccggacgacg ggaacgcggc gtcgtcggga
      121 tccgcgggcc gcgagaggcc ggcccaggac cgaacgcacg acggtgggac cgatgaccgg
      181 cccttccgcg agtttcgcgg cgcgcttgac ggcttcccgg agctcctgca cgttgccggg
      241 ccagtcgtgg gctgcgagag catccatcgc ctccacatcg aaggaaagcg tcgcgctcgt
      301 ctcctcgaga aagtgccggg cgagccgcgc gacgtcttca ccgcgctcgc gcagcggggg
      361 cagatgcagc acgcgctccg cgagacgtcg gacgatctcg tcctcgaggt ggccgcgccg
      421 gaccgcctcg accggatcgc gatccgcaga gaggatgagc cccggccgct cctcccgctc
      481 gagcgccgag agaatctctg cctggatcgc gagaggggcg cggtcgacgc cggcgaggta
      541 aacggtcccg ccgcccgcgc gcaggagcgc gtctgcgaat cgctcacccg ccgcgatttt
      601 cacgaacggc tcggcgccgc gagccgaaag tcggtgcaga aacctcgcgg ccgcctcttt
      661 ctcggtccct tcttcgccgg tcacgagaac ggcgttctcg gcggcgagcc tctcgatcgc
      721 cgccatggcc gcggtccacc gcggcgagcg cccgggcaaa gtcaccgcgc tttcgacgcg
      781 agcgagccgg agccggctct cctccgcgtc gccctcggtt ctgcgccgcc gcctcaggcg
      841 ctccaggagg agcgcgaggc gccggtcgga cacaggccgc cagagaaagt ccccggcccc
      901 cctctccatc gcctcgaccg acacttcgat tcgctcccgt ggtacgagcg cccagacggc
      961 tctccgagga tcgccggcgg cctctgcgag aaacgattca ccgacgctgt cggagagatc
     1021 cgccgccgcg acgacgacgt cgggaaggag ctcctccagg tagcgacgtc cctcggccgc
     1081 cgaggcgaca caccttgcac ggcagcctct cgcggcgaac gccaatcgca gcgcgtcgcg
     1141 ggactccgga tcgggatcga cgacgagaac tttcacggtt cgcttcggtt gcagatttta
     1201 caactaccgc cggagacgga tcccgagctc cggccaggcg gcgtcgacgc gccggcggac
     1261 ctcttcgctc atcgagatct cgtcgggcca gcggcgcgag aaaccctcct ctttccactt
     1321 gcgcgtcgcg tcgatgccga tcttgctccc gtagcacggc agccgggacg cgaagtcgag
     1381 ctcgtcgacc ggaccgtgaa cgagttcgat gtcccgctcc gggtcgatcg cggtcagcgc
     1441 ccgccagagc gcgtcgggac cgtttcggag gtccgcgtcg cgatcgaaga cgacgatcgt
     1501 cttcgtgaac atcatctgtc cggttcccca gatcgcgtgc atcgtcttgc gcgcgtgccc
     1561 ggggtagcgc ttgtcgatcg agacgagcat caggttgtgg aagatcccgt gaaccggcat
     1621 gtgcatgtcg atgatctcgg ggatcattcg gcgcacgagc ggaagaaaca gccgctcgac
     1681 ggcctcgccc atgaagccgt cctccatcgg cggcttgccg acgatcgtcg tcaggtagac
     1741 gggctcgcgc cgccgggtca ccgccgtgac gtgaaagacc gggaagtcgt cgtcgagcga
     1801 atagaagccg gtgtgatcgc cgaagggacc ttcccgccga cgctcacccg gatcgacgta
     1861 gccctcgaga acgatctcgg cctccgccgg tacgaggacg tccacggtct tcgccgcaac
     1921 gaggggaacg ctctctccgc ggaggaagcc ggcgaaaaga aactcggaga tcccgggagg
     1981 cagaggagcg atgccggaga agaccgtcgc cgggtccgcg ccgatcgcga cggcgacctc
     2041 cattctcctt ccctcccgct cgtagccccg tgcctggccg gcaccgtcct tgtgcttctg
     2101 ccagtgcatc ccggtcgtat tccgatcgaa cacctgcatc cggtagacgc cgacgttcac
     2161 ctttccgctc gaaggatctc gcgtgatcac gagcggaagc gtgatgaaag ggccggcgtc
     2221 ctggggccag caggtcagga tggggagctt cccgaggtcg acctcttccc ccgtctcgac
     2281 gatctcctgg cagggtcccg atcgcacggt cttcggaaag atctgggcga gctcggtgac
     2341 cttggggatg gttttcaact tctcgatcag ac
//
LOCUS       DASWAV010000386         3052 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U2665
            k141_622054, whole genome shotgun sequence.
ACCESSION   DASWAV010000386 DASWAV010000000
VERSION     DASWAV010000386.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960316
            Sequence Read Archive: SRR10260007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 3052)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3052)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:47:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,929
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3052
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_622054"
                     /isolate="SMAG_U2665"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.36444 N 132.31889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..286
                     /locus_tag="VF958_08030"
     CDS             <1..286
                     /locus_tag="VF958_08030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9149091.1"
                     /translation="MALVVLLLTHLLIFAPYSKRTRVLLIVGAFLSAALDEGAGWLVR
                     FVHPGFAVVKLAAFASLQGILAFLLVALALFLRAGARAEKGNGFRVTPSR"
     gene            complement(270..1481)
                     /gene="tyrS"
                     /locus_tag="VF958_08035"
     CDS             complement(270..1481)
                     /gene="tyrS"
                     /locus_tag="VF958_08035"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011366381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HEX9149092.1"
                     /translation="MTSRPSELEILKRGAVDLIEEDELERRVASKRLTVKVGFDPSAP
                     DIHLGHTVVIRKMKHFQDAGHRVVFVVGDFTGMIGDPSGRKATRPQLTRAEIEANAAT
                     YARQAFRILDPEKTSIEFNSSWLSALGAEGFVRLAGKITVARILERDDFQKRWTARQP
                     ISLHELLYPLAQGYDSIALKADVELGGTDQLFNLLVGRDLMRAEGLEPQIVMTVPLLV
                     GTDGVEKMSKSLGNAIGIEEPPSEIFGKLMSIPDRVMWNYFELLTDLPAEEIAAQKSG
                     VGEGRTNPRDVKAELARRITAQFHGAEAADRAEGDFRRAFSKGELPEKIETQEVAATS
                     HGTAARALVAVGLAGSMREARRKIAEGALSVYGPGDASRPIRDPEERLASPEPTVVRL
                     GRRFVRIIWKG"
     gene            1601..2719
                     /locus_tag="VF958_08040"
     CDS             1601..2719
                     /locus_tag="VF958_08040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9149093.1"
                     /translation="MACPVRFFPAAAALALLLAPPPSLSAETVTIPAVTSLPPGSAAS
                     PFFSDVRVFNTSYASPATVTAVYRCFLGACPATAPQVSFTLSPRESRAFDDMVFATFN
                     APSSGGAVEFTSSGSDVRVTSRLYSTAPMPTVGMFIPGLENSEAHSVSVLTSIANGAF
                     RTNIGVYNGEDSPVAVTIRLFNGTTQLGTQAVNLGPHSGTQINRIFDVVGQAGVTTTN
                     AYVIVETSDPNAEVFSYAAVIDNTTTDPIFVPGAEDVRAPSGPAPAAETINVDVSTYN
                     FAPGTSTPIQVTAGAETTLFFQSSNGTHGFSGISQLGVAGANNISAGTDGDPYGGGAH
                     APTTFRVTFTAPASARGQTYEFWCTLHPTLMRGTLHVN"
     gene            2741..>3052
                     /locus_tag="VF958_08045"
     CDS             2741..>3052
                     /locus_tag="VF958_08045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9149094.1"
                     /translation="MKTITRSIFLCAMLASGVVRAETLTVPAVTSLPVGSAASPFFYD
                     VRVFNTSYTSRVSVTAVYRCFLGACPPAAPPAPFTLAPRESLAFDDMVLATFHAPSSG
                     GA"
BASE COUNT          456 a         1061 c          985 g          550 t
ORIGIN      
        1 gatggcgctc gtcgtgcttc tcctgacaca tctcctgatc ttcgcgccgt actcgaagcg
       61 gacgcgcgtg ctgctgatcg tcggcgcctt cctctccgcc gcactcgacg agggagcggg
      121 gtggctggtc cgtttcgtcc atccgggctt tgcagtcgtc aagctcgcgg cgttcgcttc
      181 gctgcagggg atcctcgcgt tcctgctcgt ggctctggcg ctcttccttc gggccggcgc
      241 ccgggccgag aaaggcaacg ggttccgagt caccccttcc agatgatccg gacgaagcgc
      301 cttcccagcc gcacaacggt cggctcggga gacgcgagac gctcctcggg atcgcgaatc
      361 ggacgggacg cgtcgccggg tccgtagacg gagagcgcgc cttcggcgat ctttctgcgc
      421 gcctcgcgca tcgagccggc gagcccgacc gcgacgagtg cgcgcgcggc cgttccatgg
      481 gacgttgccg cgacctcctg cgtctcgatc ttctcgggca actcgccctt cgaaaacgcg
      541 cgccggaagt ccccttcggc gcggtccgcc gcctccgcgc cgtggaactg ggcggtgatc
      601 cggcgcgcga gctccgcctt cacgtcgcgc ggattcgtcc gaccttcccc gacgccgctc
      661 ttttgcgcgg cgatctcttc cgccggaaga tcggtcagga gctcgaagta gttccacatc
      721 acccggtccg ggatcgacat gagcttcccg aagatctccg agggcggctc ctcgattccg
      781 atcgcgttgc cgagcgactt cgacattttc tcgacgccgt cggtgccgac gaggagcggc
      841 accgtcatca cgatctgcgg ctccagcccc tccgcgcgca tcaggtcacg cccgacgagg
      901 aggttgaaga gctggtcggt ccccccgagc tcgacgtcgg ccttcagcgc gatcgagtcg
      961 tacccttgcg cgagcggata gagcagctcg tgcagcgata tcggctgtcg cgccgtccac
     1021 cgcttctgaa aatcgtcgcg ctcgaggatt cgcgcgacgg tgatcttccc cgcgagcctg
     1081 acgaatccct cggcgccgag cgccgagagc cagctgctgt tgaactcgat ggatgtcttc
     1141 tccgggtcga ggatccggaa cgcctgccgg gcgtatgtcg cggcgttcgc ttcgatctcc
     1201 gcgcgggtga gctgagggcg cgtcgccttc cggccggacg ggtcgccgat catcccggtg
     1261 aagtcgccga cgacgaacac gacgcggtgg cccgcatcct ggaagtgctt catcttccgg
     1321 atgacgaccg tgtgcccgag atggatgtcg ggcgcggaag gatcgaaccc caccttgacg
     1381 gtcagccgct tcgacgcgac ccggcgctcg agctcgtctt cctcgatcag atcgacggct
     1441 ccgcgcttca ggatctcgag ttcggagggg cgggaagtca cggcggccca gaatcataac
     1501 gggcgctgcc gcttcggttt aagagagggg ttaaagacgc cggccgcgcg gcctgcggcc
     1561 cgtagactcc ggcccggtga tcaaaatgaa gaaaaacacg ttggcttgcc cggttcgatt
     1621 cttcccggcg gcggcggcgc ttgcgctcct cctcgcgccg ccgccctctc tttccgcgga
     1681 gacggtgacc attccggcgg tcacgtcgct tccccccggc tccgcggctt cgccgttctt
     1741 ctcggacgtt cgcgtgttca acacgtccta cgcgagtccg gcaacggtga ccgcggtcta
     1801 ccggtgcttc ctcggggcgt gtccggcgac ggcgccgcag gtgagcttca cgctctcgcc
     1861 gcgcgagtcg cgagcgttcg acgacatggt gttcgccacc ttcaacgcgc cgtcgtcggg
     1921 gggagcggtc gagttcacga gctccggaag cgacgtccgc gtgacgagcc ggctgtattc
     1981 caccgcgccg atgccgacgg tcggaatgtt cattccaggc ctcgaaaact ccgaggccca
     2041 ctcggtgtcg gttctgacgt cgatcgcgaa cggcgcgttc cggacgaata tcggcgtcta
     2101 caacggcgag gattcgccgg tcgccgtgac gatccggctg ttcaacggaa cgactcaact
     2161 gggaactcaa gcggtgaacc tcgggccgca ttccggaacg cagatcaacc ggatcttcga
     2221 cgtggtgggt caggccggcg tgacgacgac gaacgcgtac gtgatcgtcg aaacgagcga
     2281 tcccaacgcc gaggtattca gctacgccgc ggtcatcgac aacacgacga ccgacccgat
     2341 tttcgtcccc ggggccgaag acgtacgggc gccgtcggga cccgcaccgg cggcggagac
     2401 gatcaacgtg gacgtgtcga cctacaactt cgcgccgggg acgtcgacgc cgatccaggt
     2461 cactgcgggc gcagagacga ccctgttttt ccagtcgtcg aacgggaccc acggcttctc
     2521 cgggatctca cagctgggcg tcgccggggc caacaacatt tctgcgggca cggacggcga
     2581 tccctacggc ggcggcgcac acgcgcccac gaccttccgc gtcacgttca cggcgccggc
     2641 gtccgcgcgc ggccagacct acgagttctg gtgcacgctc catccgacgc tgatgcgcgg
     2701 cacgcttcac gtcaactagg ccccgggagg taaacaactc atgaagacga tcacccgctc
     2761 gatctttctc tgcgcgatgc tcgcatccgg cgtcgttcgc gcggaaaccc tgacggttcc
     2821 ggccgtgacg tcgcttccgg tgggatcggc cgcatcgcca ttcttctacg acgtccgggt
     2881 cttcaacacc tcctacacgt cacgcgtctc ggtgacggcg gtctaccggt gcttcctggg
     2941 ggcctgtcca ccggcggcgc cgccggcgcc gttcacgctg gcgccccgtg agtcgctggc
     3001 cttcgacgac atggtgttgg ccaccttcca cgcgccgtcg tcggggggag cg
//
LOCUS       DASWAV010000387         2959 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U2665
            k141_378761, whole genome shotgun sequence.
ACCESSION   DASWAV010000387 DASWAV010000000
VERSION     DASWAV010000387.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960316
            Sequence Read Archive: SRR10260007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2959)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2959)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:47:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,929
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2959
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_378761"
                     /isolate="SMAG_U2665"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.36444 N 132.31889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..873)
                     /locus_tag="VF958_08050"
     CDS             complement(<1..873)
                     /locus_tag="VF958_08050"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEX9149095.1"
                     /translation="MNFSAGDRLGIYEIQGPLGVGGMGQVFRARDTQLGRSVAIKVLY
                     SDAARDEKRLRRLVAEARAASALNHPNILTVYGLGEQEGLPYIVTELVAGETVAQLLA
                     RSPLTLEQALDLSLQALAGLAKAHEIGIVHRDLKPANLMVTSDGFVKVLDFGLAKLSQ
                     PDSSDSEGRTLVESLGLSATEEGMIVGTAGYMSPEQVRGERVSAASDVFSMGTIIYEM
                     VTGRNPFLRETAMDTFSAILRDRPASLTESQPQISTTFSGAVDRALSKEQGERFANAR
                     EMESALTAARENVSS"
     gene            complement(887..2278)
                     /gene="sthA"
                     /locus_tag="VF958_08055"
     CDS             complement(887..2278)
                     /gene="sthA"
                     /locus_tag="VF958_08055"
                     /EC_number="1.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007511553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003957 - NAD(P)+ transhydrogenase
                     (B-specific) activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Si-specific NAD(P)(+) transhydrogenase"
                     /protein_id="HEX9149096.1"
                     /translation="MSDTIQPYDLVVIGSGPAGQKGAIAAAKLGKRVVVVDRKEMVGG
                     VCIHTGTIPSKTLREAILYLSGFRERSFYGRDYAVKEDISISDLSFRVEAVLSREVEI
                     IRAQLKRNHVELLPGTARFLDPSTIEVQSMAGTTRVSGDRFLIACGTRPSHSPTVPLD
                     GRRVIDSDQLMHAERFPKDLIVVGAGVIGLEYGSMITALNVKVTIIEQRPTILEFVDR
                     EIVDDLYYFMRQRGATFRLGEKVVSVEIDEKDRVTANLDSGKHIHGDGLLYTVGRQTN
                     ADLLNLAAAGLTADERGRIAVNEFFQTSVPHIYAAGDVIGFPALASTSMEQGRLASCH
                     MFGAYCHSRKEAIPYGIYTIPEISMVGKTEEELTAAKVPYEVGRSKFEELAKGQMLGT
                     EVGMLKILFDPKTLKLLGVHVIGESAAEIVHIGQAVLALGGTIEYFRDTVFNYPTFAE
                     AYKVAGLDGLNRL"
     gene            complement(2275..>2959)
                     /locus_tag="VF958_08060"
     CDS             complement(2275..>2959)
                     /locus_tag="VF958_08060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011793160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HEX9149097.1"
                     /translation="VSSFAEEGSLPSEVLMAAMPKIRAFEKSLPPGYRFEIAGEYKEQ
                     VKSFKNLVQVLAISVAMIFLALVLQFRSAVKPLIVFGAIPYGMVGAIGALAFMREPFG
                     FMGFLGMVSLVGVIVSHVIVLFDFIEEKHAEGEPLEQALLDAGIIRLRPVLITVGATV
                     IALVPLAMHGGPLWEPMCYAQIGGLTAATFITLLLVPVIYAIFVRDLKIVKWETVPHP
                     SPEQATLGR"
BASE COUNT          514 a          965 c          974 g          506 t
ORIGIN      
        1 agacgagacg ttctctcgcg cggccgtgag cgcggactcc atctcgcgcg cgttcgcgaa
       61 acgctcaccc tgctccttcg acagtgcccg gtccaccgcg cccgagaacg tcgtcgagat
      121 ttgcggctga ctctccgtca acgacgccgg ccgatcgcgg aggatcgccg agaacgtgtc
      181 catcgcggtc tcgcgaagga acgggttccg ccccgtgacc atctcgtaga tgatcgtgcc
      241 catcgagaag acgtcggacg cggccgagac gcgctcgccg cggacctgct ccgggctcat
      301 gtatccggca gtgccgacga tcatcccctc ctccgtcgcg gagaggccga ggctctcgac
      361 cagcgttctc ccttcggagt ccgacgagtc cggttgcgag agcttcgcga ggccgaagtc
      421 caggaccttg acgaacccgt cgctggtcac catgaggttc gccggcttca ggtcgcggtg
      481 aacgattccg atctcgtggg ccttggccag ccccgcgaga gcctgaaggc tcaagtccag
      541 ggcctgctcg agcgtcagcg gggagcgcgc gagaagttgg gcaacggttt caccggcgac
      601 gagttcggtg acgatgtagg gcagcccttc ctgctcaccg aggccgtaga ccgtgaggat
      661 gttggggtgg ttgagagccg aggccgcgcg cgcctcggcg accagccgcc gcagccgctt
      721 ctcgtcgcga gcggcgtcgg aatacaggac cttgatcgcc acactccgcc cgagctgggt
      781 gtcgcgagcg cggaagacct gccccatacc gccgacgccg agcgggccct gaatctcata
      841 gatccccagg cgatcgccgg ccgagaagtt caagagcgaa agtgcctcac agccggttca
      901 gtccgtcgag cccggcgacc ttgtaggcct cggcgaacgt cgggtagttg aaaacggtgt
      961 cgcggaagta ctcgatcgtc ccgccgagcg cgagaacggc ttgaccgatg tggacgatct
     1021 cggccgcact ctcgccgatc acgtggacgc cgagaagctt cagcgtcttc gggtcgaaga
     1081 gaatcttgag catcccgacc tcggtcccga gcatctgccc cttcgcgagc tcttcgaact
     1141 tcgaccttcc gacttcgtac gggaccttcg cggccgtcag ctcttcctcg gtcttcccga
     1201 ccatcgagat ctccgggatc gtgtagatcc cgtaggggat cgcctccttc cggctgtggc
     1261 agtacgcgcc gaacatgtgg caggaggcga gacgaccctg ctccatcgag gtcgaggcga
     1321 gcgccgggaa cccgatcacg tcgccggcag cgtagatgtg cggcaccgac gtctggaaaa
     1381 actcgttcac ggcgatccgg ccccgctcgt cggccgtgag cccggcggct gccaggttca
     1441 ggagatccgc gttcgtctgc cgccccacgg tgtagagcaa cccgtccccg tggatgtgtt
     1501 tgccgctgtc gaggttcgcc gtaacccggt ccttctcgtc gatttcgacg gacacgacct
     1561 tctccccgag ccggaaggtc gcaccgcgct gccgcatgaa gtagtagagg tcgtcgacga
     1621 tttcccggtc gacgaattcg aggatggtgg gcctctgctc gatgatcgtg accttcacgt
     1681 tcaaggcggt gatcatcgaa ccgtattcga gcccgatgac tccggcgccg acgacgatga
     1741 ggtccttcgg gaagcgctcc gcgtgcatga gctgatcgga gtcgatcacc cggcgcccgt
     1801 cgagcggcac ggtcgggctg tgagagggcc gggtgccgca ggcgatcaga aagcggtctc
     1861 cggagacgcg ggtcgtcccc gccatcgact ggacctcgat cgtgctcgga tcgagaaacc
     1921 gcgccgtccc gggcaggagc tcgacgtggt tgcgtttcag ctgcgcccgg atgatctcga
     1981 cctcccgcga gaggactgct tcgacccgga aggagaggtc ggagatcgag atgtcttcct
     2041 tgacggcgta gtcgcgcccg tagaacgagc gctcccggaa gccggagagg tagaggatcg
     2101 cttcgcgcag ggtcttcgaa gggatggtac ccgtgtggat gcagactccg cccaccatct
     2161 ccttccggtc gacgacgacg actcttttcc cgagcttcgc cgcggcgatc gcgcccttct
     2221 gcccggcggg gcccgagccg atgacgacga gatcgtaggg ctggatggtg tcgctcatcg
     2281 gccgagcgta gcctgttccg gggagggatg ggggacggtc tcccatttca cgatcttcag
     2341 gtcgcggacg aagatcgcgt agatgaccgg aaccaggagg agcgtgatga aggtcgccgc
     2401 ggtcagcccg ccgatctgcg cgtagcacat gggctcccag agcggcccgc cgtgcatcgc
     2461 gagcgggacg agtgcgatca cggtggcgcc gacggtgatg agcaccggcc gaagccggat
     2521 gattccggcg tcgagcagcg cttgctcgag cggctcccct tcggcgtgct tctcctcgat
     2581 gaagtcgaag agcacgatca cgtgcgagac gatgaccccc acgaggctca ccatgccgag
     2641 gaagcccatg aaaccgaacg gctcgcgcat gaaggcgagc gcaccgatcg cgcccaccat
     2701 cccgtagggg atcgctccga agacgatgag cggcttgacc gcgctgcgga actggagcac
     2761 gagcgccaga aagatcattg cgactgagat cgcgaggacc tggacgagat tcttgaagct
     2821 cttcacctgc tccttgtact cccccgcgat ctcgaaccgg taaccggggg gcaggctctt
     2881 ctcgaaggcc cggatcttcg gcatcgccgc catcagtacc tcggagggca gggaaccctc
     2941 ctcggcgaag gacgagacc
//
LOCUS       DASWAV010000388         2750 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U2665
            k141_260573, whole genome shotgun sequence.
ACCESSION   DASWAV010000388 DASWAV010000000
VERSION     DASWAV010000388.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960316
            Sequence Read Archive: SRR10260007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2750)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2750)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:47:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,929
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2750
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_260573"
                     /isolate="SMAG_U2665"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.36444 N 132.31889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..914
                     /locus_tag="VF958_08065"
     CDS             <1..914
                     /locus_tag="VF958_08065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9149098.1"
                     /translation="VGTNGTALTATDSAAVRGDSLATFGRVYGGRFSTASGSDGAAGV
                     IGLSSDASLAGTFSARRSGVIGVAGYGDGVRGISVFFPLGVISAGVNGTLLDTAGTFL
                     SGGVLGWSNSVGVYFVNGLAGTGTKSFVEPHPTDASKVIKFISLEGNEAGTYFRGRGR
                     FQNGIAVIEVPSEFRMVTDPDGMGIQVTPIGQMATVAVESIGLERIVVRGSRNVEFFY
                     TVNGVRRAYPNPQIIAENEKFFVPESPDARLPEYLSADERQRLIDNGTYKPDGTVNME
                     TAHRLGWDRRWEQDRTPAPQPSGPPSP"
     gene            complement(962..1342)
                     /locus_tag="VF958_08070"
     CDS             complement(962..1342)
                     /locus_tag="VF958_08070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003867663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HEX9149099.1"
                     /translation="MKFIATSSAPKAIGPYSQAVIEAGFLFSSGQVPLDPATGRLVEG
                     GLEASAERVFDNLEAVLREAGLSFADVVKTTVYLTRAEDFAAMNAVYARRFGDHRPAR
                     STVIVAELPAGAPLEIDLVARSAG"
     gene            complement(1339..1899)
                     /gene="hpt"
                     /locus_tag="VF958_08075"
     CDS             complement(1339..1899)
                     /gene="hpt"
                     /locus_tag="VF958_08075"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016284095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006166 - purine ribonucleoside salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HEX9149100.1"
                     /translation="MIERYSSPVLKLGRTLIDTKTLDAGIARLASEVDRDQAGRPALL
                     VGILKGSVFFLCDLAKRLKTPVSLDFLQVSSYGSGTRSSGNVRLVRDLTTDVAGKEVI
                     IVEDIVDTGRTLSKIVEILGTRHPKSVKICALLQKEIPGNDDVPVDYLGFRIEDVFVV
                     GYGLDHAEAFRNLPYIAVLEPDGASA"
     gene            1941..>2750
                     /locus_tag="VF958_08080"
     CDS             1941..>2750
                     /locus_tag="VF958_08080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012680679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid--tRNA ligase-related protein"
                     /protein_id="HEX9149101.1"
                     /translation="MSHPPEEPHAPPEEELIRNRRENFRRLTESGGATALYRFPATAT
                     VSEVVARHRDAAAETLQADPPSVSIAGRVLAQRHQGKAGFLDLSDGRSRLQVYVRRDV
                     VGDDAFELYRRLDLGDWIGVEGEVFRTRTGELSVKAHALTFLAKCLRPLPEKWHGLRD
                     VERRYRQRYLDLAVNPESRLVFETRAAIVRYIRRFLDERGFLEVETPMMQPIAGGALA
                     RPFKTHHNALDIDLYLRVAPELYLKRLVVGGVERVYEINRNFRNEGISTQHN"
BASE COUNT          488 a          898 c          923 g          441 t
ORIGIN      
        1 tcgtcggcac aaacggcact gccctcactg ccactgacag cgcggcggtg cggggcgaca
       61 gcctcgcgac gttcggccgg gtttatggag gacgtttctc caccgcgagc ggcagcgacg
      121 gcgcggcggg tgtaatcgga ctctcgagcg atgcctccct cgccggaacg ttcagcgccc
      181 ggcgaagcgg cgtaatcgga gtggccgggt atggcgacgg agttcgtggc atcagcgtct
      241 tcttccccct cggcgtcatc agcgcgggcg tcaacggcac gctgttggac accgcgggga
      301 cgtttctttc gggcggcgtc ctcggctggt cgaactcggt cggtgtgtat tttgtaaacg
      361 gcctcgcggg cactgggacc aagagcttcg tcgagccgca cccgaccgac gcctccaagg
      421 tcatcaagtt catctcgctc gagggaaacg aggccggaac ctacttccgg gggcgcggga
      481 gattccagaa tggcatcgcc gtcatcgagg tgccctcgga gttccggatg gtgaccgacc
      541 cggacgggat gggcatccag gtgacaccga tcggccagat ggccacggtc gccgtggaat
      601 cgatcgggct ggagcggatc gtcgtgagag gttcccgcaa cgtcgagttc ttctacacgg
      661 tcaacggcgt tcgccgcgca tacccgaacc cacagatcat cgccgagaac gagaagttct
      721 tcgttccgga atcgcctgat gcccgcctgc cggagtacct gagcgcggac gagaggcagc
      781 ggttgatcga caacgggacg tacaagccgg acgggacggt gaacatggag acagcacacc
      841 gcctgggttg ggatcgacgt tgggagcaag accggacacc cgcgccgcaa ccgagcggtc
      901 ccccgagtcc ctgaggcggc cttccgattc tgatattgcg ccgcggagtg agacctcccg
      961 actatcccgc cgagcgtgcg acgaggtcga tctcgagagg tgcgccggcg ggaagctcgg
     1021 ccacgatgac cgtcgagcgc gcggggcggt gatcgccgaa gcggcgcgcg tagaccgcgt
     1081 tcatcgcggc gaagtcctcc gcgcgggtca ggtacacggt ggtcttgacg acgtcggcga
     1141 acgagaggcc cgcctcccgg agaaccgcct cgaggttatc gaagacgcgc tccgcggaag
     1201 cctcgaggcc gccctcgacg aggcgacccg tcgccggatc gagcgggacc tgcccggacg
     1261 aaaagaggaa cccggcctcg atgacggcct gggagtacgg gccgatcgct ttcggagcgg
     1321 agctcgtcgc aatgaacttc atgccgacgc accgtcgggc tcgaggacgg cgatgtaggg
     1381 aaggttgcgg aacgcctcgg cgtgatcgag gccgtatccc acgacgaaga cgtcttcgat
     1441 ccggaatccg aggtagtcga cggggacgtc gtcgttgccg gggatctcct tctgcagcag
     1501 ggcgcagatc ttcaccgact tcggatgccg cgtcccgagg atctcgacga tcttcgagag
     1561 cgtccggccg gtatccacga tgtcctcgac gatgatgacc tccttcccgg cgacgtcggt
     1621 cgtcagatcc cgcacgagac ggacgttccc cgaagaccgg gttccggagc cgtaggacga
     1681 gacctgcaga aaatcgagcg acaccggagt cttcagcctc ttcgcgagat cgcagaggaa
     1741 aaagaccgaa cccttgagaa ttccgacgag cagcgcgggc cggccggcct ggtcgcgatc
     1801 cacctcgctc gcgaggcgcg cgatccccgc gtcgagggtt ttcgtgtcga tgagcgtgcg
     1861 gccgagcttc aagacgggcg aagagtatct ctcgatcacg aatgcgttca aggcacttcg
     1921 gacccgtata ctcccggccc atgagtcatc ctccggagga accgcacgca ccgcccgaag
     1981 aggagctcat tcgcaatcgg cgcgagaact tccgacgtct gacggagagc ggcggcgcga
     2041 cggcgctcta tcggttcccg gcgacggcga ccgtctcgga ggtcgtagcc cgccaccgcg
     2101 acgcggcggc ggagactctc caggccgatc cgccgtccgt ctcgatcgcg ggccgggttc
     2161 tggcgcagcg ccatcaggga aaggccgggt tcctcgacct ctccgacggg cggagccgtc
     2221 ttcaagtcta cgtccgccgc gacgtcgtcg gcgacgacgc cttcgagctc tatcgtcggc
     2281 tcgacctcgg cgactggatc ggagtcgaag gtgaggtgtt ccgcacgcga accggagagc
     2341 tttccgtgaa ggcgcacgct ctgacgtttc tcgccaagtg cctgcgcccg ctgcccgaga
     2401 agtggcacgg cctccgcgac gtcgagcgtc ggtatcggca gaggtacctc gacctcgccg
     2461 tgaacccgga atcgcgcctc gtcttcgaga cgcgcgccgc gatcgtccga tacatccggc
     2521 gctttctcga cgagcgcggg ttcctcgagg tcgagacgcc aatgatgcag ccgatcgccg
     2581 ggggcgccct cgcgcgacct ttcaagaccc accacaacgc gctcgacatc gacctctacc
     2641 tgcgcgtggc cccggagctc tacctgaagc ggctcgtcgt cggcggggtg gagcgcgtgt
     2701 acgagatcaa ccgcaacttc cgcaacgagg ggatctcgac gcaacacaat
//
LOCUS       DASWAV010000389         4151 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U2665
            k141_422002, whole genome shotgun sequence.
ACCESSION   DASWAV010000389 DASWAV010000000
VERSION     DASWAV010000389.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960316
            Sequence Read Archive: SRR10260007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 4151)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4151)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:47:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,929
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4151
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_422002"
                     /isolate="SMAG_U2665"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.36444 N 132.31889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(52..1071)
                     /locus_tag="VF958_08085"
     CDS             complement(52..1071)
                     /locus_tag="VF958_08085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007413769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YncE family protein"
                     /protein_id="HEX9149102.1"
                     /translation="MLEVLVAVLLGLASSPAPAAAPGYHLVKTLRVGGDGGWDYLTVD
                     VAARRLYVTHSTRVVVLNADSGERIGEIPNTPGVHGVALAPELDRGFTSNGRSSTLTV
                     FDLKSLKTLSEIKATGENPDAILYDPVSRRVFTFNGRSANATAIDAAGGTVAGTIPLD
                     GKPEFAVSDGRGRVFVNIEDKSVIDVIDPRKLSVEKRWPLAPCEEPSGMAIDREHRRL
                     FVGCRNRMMAVVDAENGRVVTSVKIGEGVDANAFDPVSGLAFASCGDGTLTVAHEDSP
                     DKLTVVQKVETQRGARTMAFDDKSRNVYLATAQFGPAPAPTAGQPRPRPSILPDTFVV
                     LVLGR"
     gene            complement(1075..2544)
                     /locus_tag="VF958_08090"
     CDS             complement(1075..2544)
                     /locus_tag="VF958_08090"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HEX9149103.1"
                     /translation="MFLRRVACALLLSAFTAFAFAAEPPSAKSVANDPRVQSALELAR
                     TWLEAQRAYDRIPGVSAEIVYDQEVLWSGGFGLADVEAKRPATTETIYSICSISKLFT
                     SVAVLQQRDEGKIHLEDSIGKYLPWFHLKRTEGEGDVTVEGILTHASGLPRESDFPYW
                     SAPDFKFPTHEEIVSKISSQEALYTPEEHFQYSNLGLTLAGEIVAATSGMPYEDYARR
                     RVLDPLDLASTTPQMPEAERGKKLATGYTALDRDGERRALPFFTAKGIAPAAGYASTV
                     GDLGRFASWQFRLLSKGGTEVLKATTLREMYRIHWAEPDFETLWGLGFEIWKSGDSKM
                     FVGHGGSCPGYRSHLLLMPGEKVATVIMANGQGVATEKFAQRLYDIVAPAIRDAVKEP
                     GKAKVPDPSLRRYAGAYDIQPWGGEVAVLPWEDGLAMVGFPTMDPVKELDRFRKVGEH
                     RFRRIRKDDSLGEEIVFDVGPDGRATQFTQHSNHYSRVR"
     gene            complement(2664..>4151)
                     /locus_tag="VF958_08095"
     CDS             complement(2664..>4151)
                     /locus_tag="VF958_08095"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX9149104.1"
                     /translation="HFRLLVDGVEEYAIFMLDSQGNVMSWNRGAERIKGYRASEIIGT
                     HFSRFYPNQSVLEGKPAWELEMATLYGSFEDEGWRIRKDGSRFWANVVITALRDKAGR
                     LRGFAKVTRDMTERRRIEDLENADRQKDQFLALLAHELRNPIAPIRTALEILRRPNAT
                     PAETDQARDIAERQLRHMARLLDDLLDVSRIREGRIELRMETLDVASVMRSAAQAAAS
                     LIQARNHTLTIVDPKDRLFVRADPVRLEQVLGNLLNNAAKYTDPGGQIRMTAERIAGE
                     ALIRVRDTGIGIEPLMIPRVFDLFVQAERRMELSAGGVGIGLSLVKKLVELHGGRVEA
                     ISAGSGRGSEFIVALPAVEQKPVERVTAAETPSTQLARAPLRVLLVDDNADSADGLAM
                     LLELQGHETRVAYDGESALGIARSFRPHAALLDIGMPAMDGYELARRLRAAPETKETM
                     LVAMTGWGQEEDQRKGREAGFAHHLVKPFEPSAVERLLADFDPAG"
BASE COUNT          655 a         1412 c         1350 g          734 t
ORIGIN      
        1 tcgaagtgag gggaatcttc gtccggcttc cgcggcgggc caggtaaggc gttacctccc
       61 gagtaccagc acgacgaaag tgtccgggag gatgctcggg cgagggcgag gctgcccagc
      121 ggtcggcgcc ggggcgggac cgaactgcgc ggtcgcaaga tagacgttgc gggacttgtc
      181 gtcgaacgcc atcgtgcgcg cgccacgctg tgtctcgacc ttctggacga ccgtgagttt
      241 gtcgggcgaa tcctcgtggg cgaccgtgag cgttccgtcg ccgcaggatg cgaacgccag
      301 cccgctgacg ggatcgaacg cattcgcatc gacgccttcg ccgatcttga ccgaggtgac
      361 gacgcggcca ttttcggcgt cgacgacggc catcatccgg ttgcggcagc cgacgaagag
      421 ccgccggtgc tcgcggtcga tcgccatgcc ggaaggctct tcgcacggcg cgagcggcca
      481 gcgcttctcc acggagagct tccggggatc gatcacgtcg atgacgctct tgtcctcgat
      541 gttgacgaac actctcccac gtccgtcgct caccgcgaac tcgggctttc catcgagtgg
      601 gatcgtgccc gcgacggtcc cgccggcagc atcgatcgcc gtcgcattcg cgctccttcc
      661 gttgaaagta aagaccctgc gcgacacagg gtcgtagagg atcgcgtccg ggttctcgcc
      721 ggtcgccttg atctcggaaa gtgtcttcag gctcttcagg tcgaagacgg tgagcgtcga
      781 cgagcgcccg ttgctcgtga agccgcgatc gagctccggc gccagcgcga cgccatgcac
      841 accgggagtg ttgggtatct cgccgattcg ctcgccgctg tcggcgttga ggacgacgac
      901 gcgtgttgaa tgcgtcacgt agagccggcg tgccgcgacg tcgacggtga ggtagtccca
      961 tcccccgtcc cccccgacgc ggagcgtctt cacgaggtga tatccgggcg cggcggcggg
     1021 cgcggggctc gaggccagcc cgagcagtac ggcgacgaga acttcgagca tggcctacct
     1081 cacccgggag tagtggttgc tgtgctgcgt gaattgcgtc gcgcggccgt ccgggccgac
     1141 gtcgaagacg atctcttcgc cgagcgagtc gtccttgcgg atccggcgga accggtgctc
     1201 gccgaccttc ctgaaccggt ccagctcttt cactggatcc atcgtcggga agccgaccat
     1261 cgcgaggcca tcctcccagg gaaggaccgc gacttcgccg ccccagggct gaatgtcgta
     1321 cgcacccgcg tacctccgca gtgagggatc cggcaccttc gccttccccg gctccttcac
     1381 ggcgtccctg atcgccggcg cgacgatgtc gtagaggcgc tgcgcgaatt tttccgtcgc
     1441 gacgccctgg ccgttcgcca tgatcacggt cgcgactttc tcgcccggca tcagcaggag
     1501 gtgggagcga tagcccgggc agctgccacc gtgtccgacg aacattttcg aatcgccgct
     1561 cttccagatc tcgaagccga gtccccacag ggtctcgaag tcgggctccg cccaatggat
     1621 gcggtacatc tcgcgcaggg tcgtcgcctt caggacctcg gtgccgccct tcgagagaag
     1681 ccggaactgc caggacgcga acctcccgag gtctccgacc gtcgacgcat accccgccgc
     1741 cggggcgatc cctttcgcgg tgaagaacgg aagcgcgcgc cgctcaccat cgcggtcgag
     1801 cgccgtgtat ccggtcgcga gcttcttgcc ccgctcggcc tccggcatct ggggcgtcgt
     1861 gctcgcgaga tcgagcggat cgaggacgcg gcgacgcgcg tagtcctcgt acggcatccc
     1921 cgacgtcgcc gcgacgatct cccccgcgag ggtcagcccg aggttcgagt actggaagtg
     1981 ctcctcgggc gtgtacagcg cctcctggct cgaaatcttc gagacgatct cctcgtgagt
     2041 cggaaacttg aagtccggcg ccgaccagta aggaaagtcc gactcgcgcg gcagacccga
     2101 cgcgtgcgtc aggatccctt cgaccgtgac gtcaccctcc ccttccgtgc gcttcaggtg
     2161 aaaccacggg aggtacttgc cgatcgagtc ttccaggtgg atcttgccct cgtcgcgctg
     2221 ctgcaggacg gcgacgctcg tgaagagctt cgagatcgag cagatgctgt agatggtttc
     2281 cgtcgtcgcg ggacgcttcg cctccacgtc cgccagaccg aacccgccgc tccacaggac
     2341 ctcctggtcg tacacgatct ccgcggagac gcccgggatc cggtcgtatg cccgctgagc
     2401 ctcgagccac gttcgcgcga gctcgagcgc cgactgaacg cgcggatcgt tcgcgacgct
     2461 cttcgccgag gggggctccg cggcgaaggc gaatgccgtg aaggctgaca gaaggagcgc
     2521 acacgccacg cggcggagga acatcgtcac ctcccgcaag atcgtccgga ttttctcatc
     2581 atcgcgagag attctcccga tcgatccgag ggtcgagtcg cgcagtgtgg ctgcgctcgc
     2641 gaagcaatgt ggcaagtgga gacctacccc gcagggtcga agtccgcgag gagcctttcg
     2701 accgcggagg gttcgaacgg cttgacgagg tggtgcgcga acccggcctc gcgtcccttg
     2761 cgctggtcct cctcctgtcc ccacccggtc atcgcgacga gcatcgtctc cttcgtctcc
     2821 ggggcggcgc gcagccggcg agcgagctcg tagccgtcca tcgccggcat tccgatgtcg
     2881 agcagcgccg cgtgcggacg gaagctgcgc gcgattccga gggcgctctc gccgtcgtag
     2941 gcgacccggg tctcgtgccc ctggagctcc aggagcatcg caagcccgtc cgccgagtcg
     3001 gcgttgtcgt cgacgaggag gacccgcagg ggcgctcgcg cgagctgcgt ggacggcgtc
     3061 tcggcagccg tgacgcgctc gaccggcttc tgttcgactg ccggcagtgc cacgatgaac
     3121 tcgcttcccc ggcccgaacc ggcgctgatc gcctcgacac ggcccccgtg gagctcgacc
     3181 aacttcttca cgagcgacag tccgatcccc acgcctcccg cagagagctc catgcgcctc
     3241 tcggcctgaa cgaagaggtc gaagacacgg gggatcatca gcggttcgat cccgatcccg
     3301 gtgtcgcgca cgcggatgag cgcctcgccg gcgatcctct ccgccgtcat ccggatctgt
     3361 ccgcccgggt cggtgtattt cgcggcgttg ttcaggaggt ttccgagtac ctgctcgagt
     3421 cgaacgggat ccgccctcac gaacagccgg tctttcggat cgacgatcgt cagcgtatgg
     3481 ttgcgcgcct ggatcaaaga agctgccgcc tgcgccgccg accgcatgac cgatgcgacg
     3541 tcgagggtct ccatccgcag ctcgatccgg ccttcgcgaa tccgtgaaac gtcgagcagg
     3601 tcgtcgagaa gccgcgccat gtgacgcagc tgacgctcgg cgatgtcccg cgcctggtcg
     3661 gtctcagcgg gcgtggcgtt cggccgccga agaatctcga gggccgtccg gatgggcgcg
     3721 atggggttgc gcagctcgtg ggcaagcagc gccaggaact ggtccttctg ccggtcggcg
     3781 ttctccagat cttcgatccg gcgccgctcg gtcatgtccc gggtcacctt cgcgaagccg
     3841 cgcagtctgc cggccttgtc gcgaagcgca gtgatgacga cgttcgccca gaaccgggac
     3901 ccgtcctttc ggattctcca gccctcgtcc tcgaagcttc cgtaaagagt cgccatctcg
     3961 agctcccagg cgggtttccc ctcgaggacc gactggttcg gatagaagcg cgagaaatgc
     4021 gttccgatga tctcggacgc gcggtagccc ttgatcctct ccgcaccgcg attccagctc
     4081 atgacgttgc cctgcgaatc gagcatgaag atcgcgtatt cctccacgcc gtcgacgaga
     4141 agccggaaat g
//
LOCUS       DASWAV010000390         1938 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U2665
            k141_780012, whole genome shotgun sequence.
ACCESSION   DASWAV010000390 DASWAV010000000
VERSION     DASWAV010000390.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960316
            Sequence Read Archive: SRR10260007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 1938)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1938)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:47:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,929
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1938
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_780012"
                     /isolate="SMAG_U2665"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.36444 N 132.31889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(351..932)
                     /locus_tag="VF958_08100"
     CDS             complement(351..932)
                     /locus_tag="VF958_08100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9149105.1"
                     /translation="MGILNRRPHFALFIAAVAFAIGVAPALAKSKTFQNSDDAKEANE
                     PAKYLPDYDKLIEGKDADWVYFPEGSLKKYKTVTVKDFVENGKGREAREAARAGKEYM
                     EQWLERGGYKLVDKGAELTIEGNVFNAWEPGTGARIWGGWMANPGVGVEVIARDSSGK
                     VVGEIRHKNRGSTVEDAVENALEEVAKAISDGR"
     gene            complement(973..>1938)
                     /locus_tag="VF958_08105"
     CDS             complement(973..>1938)
                     /locus_tag="VF958_08105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020478230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate/isopropylmalate family dehydrogenase"
                     /protein_id="HEX9149106.1"
                     /translation="RSLPFEPVFFPWSADHYLSTGETVPAGAFDDLSAGYDAIFMGAF
                     GDPRVPDMRHAAEILLGARFTLDLYVNYRPVRCLDDRLCPLKGYTAADVDFVVFRENT
                     EGSYVGAGGHLKKGTSDEVAVQEDVSTRKGVERIVRYAFEYARSRGRKRVVMSDKSNA
                     MRYVGDLWQRVFAEVSREYPDLESSHLYIDALCMQMVKDPRQFDVIVTNNMFGDIATD
                     LGAQLQGGLGMAASGNIHPGRVSMFEPVHGSAPKYAGTGRANPFGAILTAGLMLEHLG
                     HRAEAEALERAVARCLREGRATADVGGSLTTSQAGDAVCERLEFG"
BASE COUNT          298 a          661 c          621 g          358 t
ORIGIN      
        1 catcgtggcg agctcggaga acgctgaaag gagcttccgt gcgctttcct ctccctctcc
       61 gccgacgacc gagacctcgt tgccgcgggc ggcgaggcgg actcggaacg cctcctcgat
      121 cgccctcagg ttctcgtccg cgatgccgaa aagggcggta aatcccgcct cgggaagcgt
      181 gacggtcggg ttcgggtcgg gaatcttggt tactcctccg gtccggaaat tacgctacca
      241 cgcccaaaag gcagcaaaag gcgggccccg ttgccgggga cccgcctctc gacggcaaac
      301 ctattgtcca gaaggcttag atcggcgccg cgtttttcct cgctcggccg tcagcggccg
      361 tccgagatcg ccttggcgac ctcctcgagg gcgttctcga cggcgtcttc gaccgtcgag
      421 ccccggttct tgtgccggat ctcgccgacc acctttccgc tggaatcgcg ggcgatgact
      481 tcgactccga ctcccgggtt ggccatccag ccgccccaga tccgggcgcc ggtgccgggc
      541 tcccaggcat tgaacacgtt gccctcgatc gtcagctccg ctcccttgtc gacgagcttg
      601 tagccgcccc gctcgagcca ctgttccatg tactccttgc ccgcacgggc cgcctcgcgc
      661 gcctcgcgtc ccttgccgtt ctcgacgaag tccttcacgg tgacggtctt gtacttcttc
      721 aggctcccct ccgggaagta gacccagtcg gcgtctttcc cctcgatgag cttgtcgtag
      781 tcgggcaggt actttgccgg ctcgttggct tccttggcgt catcagagtt ctggaaggtc
      841 tttgacttcg cgagcgcggg cgcaacgccg atcgcgaaag caacggcggc gataaacagg
      901 gcaaagtgcg gacgacgatt cagaatcccc atggctttga ttctcctttg acggcgcatt
      961 ttacgcggtc ggctatccga actccagccg ctcgcagaca gcgtcgcccg cctgcgacgt
     1021 cgtgagcgac cctcccacgt ccgcggtcgc gcggccctcc cgcaggcagc gggcgaccgc
     1081 acgctccagc gcttcggctt ccgcccggtg tccgaggtgc tcgagcatga ggcccgccgt
     1141 caggatcgcc ccgaacgggt tcgcccgacc ggtgccggcg tacttgggag ccgagccgtg
     1201 gaccggctcg aacatcgaga cccgcccggg atggatgttg cccgacgccg ccatcccgag
     1261 tccgccctgg agctgcgcgc cgaggtcggt cgcgatgtcg ccgaacatgt tgttggtgac
     1321 gatgacgtcg aactggcggg ggtccttgac catctgcatg cagagagcgt cgatgtagag
     1381 gtgggaggat tcgagatccg ggtactcgcg ggaaacctcc gcgaagacgc gctgccagag
     1441 gtcgccgacg taccgcatcg cgttcgactt gtcggacatc acgacccgtt ttcgtccgcg
     1501 cgagcgggcg tactcgaaag cgtagcggac gatcctttcc actccctttc gcgtcgagac
     1561 gtcctcctgg acggcgacct cgtccgacgt ccccttcttc aggtgaccgc ccgcgccgac
     1621 gtacgacccc tccgtgttct cgcggaagac gacgaagtcg acgtcggctg ccgtgtagcc
     1681 cttgagcggg cacaggcggt cgtcgaggca tcggacgggg cggtagttga cgtacaggtc
     1741 gagcgtgaat cgcgcgccga ggaggatctc ggccgcgtgc cgcatgtccg ggacgcgcgg
     1801 gtcgccgaag gcgcccatga agatcgcgtc gtagcccgct gacaggtcgt cgaacgctcc
     1861 cgccgggacg gtctcgcccg tcgagaggta gtggtccgcg ctccacggga agaagacagg
     1921 ttcgaaaggc agcgatcg
//
LOCUS       DASWAV010000391         3737 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U2665
            k141_422140, whole genome shotgun sequence.
ACCESSION   DASWAV010000391 DASWAV010000000
VERSION     DASWAV010000391.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960316
            Sequence Read Archive: SRR10260007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 3737)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3737)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:47:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,929
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3737
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_422140"
                     /isolate="SMAG_U2665"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.36444 N 132.31889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            1..213
                     /locus_tag="VF958_08110"
     CDS             1..213
                     /locus_tag="VF958_08110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013718813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HEX9149107.1"
                     /translation="MDRYTIVIRPDDNGTFVAYLPALPGCHALGRTAAEAQAELANVF
                     SMVAEEYAEEGRPLPPDVPVLAANAG"
     gene            203..415
                     /locus_tag="VF958_08115"
     CDS             203..415
                     /locus_tag="VF958_08115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006195784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
                     /protein_id="HEX9149108.1"
                     /translation="MPAKAGDLERVAKKLGFIKARQRGSHARWKHPDGRATTIPIHGS
                     AEVGGWLFHEILAQPGISEEQFRETQ"
     gene            433..810
                     /locus_tag="VF958_08120"
     CDS             433..810
                     /locus_tag="VF958_08120"
                     /EC_number="4.1.2.50"
                     /inference="COORDINATES: protein motif:HMM:NF013411.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070497 -
                     6-carboxy-5,6,7,8-tetrahydropterin synthase activity
                     [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase"
                     /protein_id="HEX9149109.1"
                     /translation="MPYRVTVSARFEAAHNLIDYAGGPEPLHGHSYKVEAVLESDKLQ
                     QYDLAIDFVAAKRALEAIAKELDYRYINEHPDFAVRNTSAENLARWFAERLDSSGVLG
                     WTRVAEVTVWEGPENRATYVPKP"
     gene            1120..1863
                     /locus_tag="VF958_08125"
     CDS             1120..1863
                     /locus_tag="VF958_08125"
                     /inference="COORDINATES: protein motif:HMM:TIGR02532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HEX9149110.1"
                     /translation="MRTRKVGRRHELGMTLLEVSITIAILGTLALLAAPSAAKLIRRS
                     QALAAYSSMQQVLASARMQAVKRGVNVVVLVDVTPEKKIHMHTFQDRANKETALDTTE
                     QDAVGNFKQDTFVGGVTDEPTLGDVVLPATVAIWKQGGTKDDIVPGIAFDTYTPPTPL
                     PPSPDSSLTDRVVFVPTGGIAPPQDSNSGLPTPTGGRGVYFADSSGKNFFRVTVDSDI
                     SGRIVVDKYMPTATARYSTGYRPTEWVWY"
     gene            1854..2429
                     /locus_tag="VF958_08130"
     CDS             1854..2429
                     /locus_tag="VF958_08130"
                     /inference="COORDINATES: protein motif:HMM:NF019575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HEX9149111.1"
                     /translation="MVLRPSARRRESGLTLVEVLVATVILGLALLALAPMFTNAVRSN
                     ASAYQLTNTNTLAREKLEELSGYPRSDPRLAVAAPNNASGPTTASVGTGSIVGVNAWC
                     QNDLPTWYNPQTGATSTAATSPGVGWFSYPFTRTYTIEQFDSDGTGMTRVVAPGAYVV
                     KLITVTVRPTSGPFPGLRESRQALYLRVKDA"
     gene            2422..3723
                     /locus_tag="VF958_08135"
     CDS             2422..3723
                     /locus_tag="VF958_08135"
                     /inference="COORDINATES: protein motif:HMM:NF027400.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0043683 - type IV pilus assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilW family protein"
                     /protein_id="HEX9149112.1"
                     /translation="MRRRNYSKGLTLIETLITLAVLTAVMVGLYALLDSSNKLAKQET
                     NVAEAQQSVRIGISEVTRVIRQGRVGGLFIANAILPVANNSGAGGTLTDISGTAHYIR
                     AGTDVIGVRGIISGDKYGLATGDVTCGGSCATTTAMTVTIRATSSSGVVNFLSGTAPA
                     IAGKSRPFYFVVADGSTEMVTTGVGTYLVPVYYVGLVDANSSGTWFTQTSNTFTFSMN
                     PQDSGALKLTAAPPSSTTLDKPFSGGVVDEIRFFVDEGPTDASTSALDTHPSLAEAIF
                     DPSSGNWEIQPLIEDVEDFQVAYGVDGIDGTAHDHGISPGAYDTSGSNRDEWVGNVAN
                     EVTTTLTLTSSDPKRVEAFIDSSIPTGPSAPAIAGAALRSVMVGLVVKSADPDFRYNG
                     PGARGFSLLDSTARSFSSASSTGRPYRRRLQRFAVSLRNYQ"
BASE COUNT          710 a         1236 c         1154 g          637 t
ORIGIN      
        1 ctggaccgct acacgatcgt catccgaccg gatgacaacg ggactttcgt cgcctatctg
       61 ccggcgctcc cgggctgcca cgccctggga cggaccgccg cggaggccca ggccgaactt
      121 gcaaacgttt tcagcatggt cgctgaggaa tacgcagaag aaggtcgtcc acttccaccc
      181 gacgtgcccg tcctggccgc gaatgccggc taaggccggc gatctggagc gcgtcgcgaa
      241 gaagcttggg ttcataaagg ctcgccagag gggcagccat gctcgatgga aacaccccga
      301 cggtcgcgcc acaacgattc caatccacgg ttcggcggaa gtcggcggct ggctgttcca
      361 cgagattctt gctcagcctg gaatctccga ggaacagttc cgagaaactc aatagcgaag
      421 acgtccaatc gaatgcccta tcgggtcacc gtttccgccc gcttcgaagc ggcccacaac
      481 ctgatcgact acgccggcgg ccccgagccg ctccacgggc actcgtacaa ggtcgaggcg
      541 gtgctcgaga gcgacaagct ccagcagtac gacctcgcga tcgatttcgt cgccgcgaag
      601 cgcgcgctcg aagcgatcgc gaaggagctc gactaccgct acatcaacga gcatcctgac
      661 ttcgcggtgc gcaacacgtc cgccgagaac ctcgcgcgct ggttcgcgga gaggctcgac
      721 tcgtccggcg ttctcggctg gactcgcgtc gccgaggtga ccgtctggga aggacccgag
      781 aaccgcgcga cgtacgtgcc gaaaccctga ccgtcccgct tggacagtgg accttccgag
      841 cagcctcggc aggaccattg gggcccgacg gtcccgttcg gcatccgcgg tgttacgttc
      901 ggtaacactt tttcgcgaaa gccattcgtg aggactgtca cgaaaggcgc gtaagcgttt
      961 gttctaaaat ccattagacc tcagtcgaag gcgcggcacg gcattcgcta tatacctgtt
     1021 tgctcccgga aagagtgcgg acccaaagtt cttggactgt aaagaattag ccggaacgga
     1081 aaaggtggac tgggacaagt gtcaccacgg aagcaagcgg tgagaacccg gaaggtcgga
     1141 cggcgtcacg agctcggaat gaccctcctg gaggtctcga tcacgatcgc aattctggga
     1201 acgctggcgc tcctcgcggc cccgtcggcc gccaagctca ttcgccgctc gcaggcgctg
     1261 gcggcttact catcgatgca acaggttctc gcgtcggcgc ggatgcaggc agtcaagcgc
     1321 ggagtgaacg tcgtcgtgct ggtggacgtg acccccgaaa aaaagatcca tatgcatacg
     1381 ttccaggacc gagcgaacaa ggagacggcg ctcgacacca ccgaacagga cgcggtcggg
     1441 aacttcaagc aggacacgtt cgtcgggggc gtcaccgacg agccgaccct cggagacgtg
     1501 gtcctcccgg ccacggttgc catctggaaa cagggaggga cgaaggacga catcgttccg
     1561 ggaattgcgt tcgacacgta cacgcccccg acacccctac ccccttcccc ggattcctct
     1621 ctgaccgacc gcgtcgtgtt cgttcccacc ggcgggattg cccctcctca agactccaac
     1681 tcgggcctgc caaccccgac gggaggaagg ggggtctact tcgccgactc ctcgggaaaa
     1741 aacttcttcc gcgtgactgt ggatagcgac atctccggac ggatcgtcgt cgacaaatac
     1801 atgccgacgg cgaccgcacg ttattcgacc ggttaccggc cgacggaatg ggtatggtac
     1861 tgagaccgtc tgcccgtcga cgcgagagcg gcctgaccct cgtcgaggtg ctggtggcga
     1921 cggtcattct cggcctggcg ctgctcgctc tcgctccgat gttcaccaac gcggtgcgga
     1981 gcaacgcctc cgcctaccag ctcaccaaca cgaacacgct cgcccgcgag aagctcgaag
     2041 agctctccgg ctacccgagg agcgatcccc gcctcgccgt cgccgccccc aacaacgcgt
     2101 cgggtccgac gactgcctcc gtgggcaccg gaagcatcgt cggcgtgaac gcgtggtgcc
     2161 agaacgatct ccccacctgg tacaacccgc agaccggcgc cacgtccacc gccgcgacta
     2221 gcccgggagt gggctggttc tcttacccgt tcacgcgcac ctacacgatc gagcagttcg
     2281 attccgacgg gacaggcatg acgcgtgtgg ttgcgcccgg ggcctacgtg gtcaagctga
     2341 tcacggtgac cgtgcggccg accagcggac cgttcccggg tttgagagag agccggcagg
     2401 ctctctatct gagggtcaag gatgcgtagg cgcaattact cgaagggcct caccctgatc
     2461 gagaccctga tcacgctcgc cgttctgacg gcggtcatgg tcggcctcta cgcgctactc
     2521 gactcgtcga acaagctcgc gaagcaggag acgaacgtcg cggaggcgca gcagtcggtg
     2581 cggatcggga tctcggaagt cacgcgggtg atccgccagg ggcgggtcgg cggccttttc
     2641 attgcgaacg cgattctgcc ggtcgcgaac aacagcgggg ccggcggcac gctgaccgac
     2701 atctccggga ccgcgcatta catccgcgcg ggcaccgacg tgatcggcgt gcgcggaatc
     2761 atctcgggcg acaagtacgg cctggcgacg ggagacgtga cctgcggggg gagctgcgcg
     2821 acgaccaccg cgatgaccgt cacgatccgc gcaacctcct cgagcggggt cgtgaatttt
     2881 ctttcaggga ctgcgccggc gatcgccgga aagagccgtc ctttctactt cgtcgtcgca
     2941 gacggatcga ccgagatggt gacgacgggc gtgggcacct acctcgtgcc ggtctattac
     3001 gtcggcctcg tcgatgcgaa ttcgagcggt acgtggttca cgcaaacctc gaacaccttc
     3061 accttctcga tgaacccgca ggactcgggc gctttgaagc tgacggccgc cccaccgtcg
     3121 tcgaccacgc tcgacaaacc tttctcgggg ggggtcgtgg acgaaatccg ctttttcgtc
     3181 gatgagggtc caaccgacgc cagcacgagc gcgctcgata cgcatccttc cctcgcggag
     3241 gcgatcttcg acccgtcgtc gggaaactgg gagattcagc cgctgatcga agacgtcgag
     3301 gacttccagg tcgcctacgg cgtggacgga atcgacggaa ccgcccacga tcacggaatc
     3361 tcgccggggg cctacgacac gagcggctcg aaccgcgacg aatgggtcgg aaacgtcgcg
     3421 aacgaggtca cgactacgct cacgctcacc tcgtccgacc ccaagcgcgt cgaggctttc
     3481 atcgattcct cgatcccgac ggggccctct gcgccagcga tcgcgggggc ggcgttgcgc
     3541 tcggtcatgg tcggcctcgt cgtcaaatcg gccgaccccg acttccggta caacggcccc
     3601 ggagcgcgtg gtttcagtct tctcgactcg acggcgcggt ccttctcgag cgcgtcatcc
     3661 actggccgcc cctaccggcg gcgacttcag agattcgccg tttcgcttcg caattaccaa
     3721 tgagctctcc cgagttc
//
LOCUS       DASWAV010000392         4375 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U2665
            k141_260956, whole genome shotgun sequence.
ACCESSION   DASWAV010000392 DASWAV010000000
VERSION     DASWAV010000392.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960316
            Sequence Read Archive: SRR10260007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 4375)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4375)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:47:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,929
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4375
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_260956"
                     /isolate="SMAG_U2665"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.36444 N 132.31889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..231
                     /locus_tag="VF958_08140"
     CDS             <1..231
                     /locus_tag="VF958_08140"
                     /inference="COORDINATES: protein motif:HMM:NF015415.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase small subunit"
                     /protein_id="HEX9149113.1"
                     /translation="MAAVRSDRPRIALGSVAPTVVRLPETEAALAAGAPIAEARRVLE
                     VEIRPIDDLRSTAEYRRRVSGNLLEQFWRETS"
     gene            228..1577
                     /locus_tag="VF958_08145"
     CDS             228..1577
                     /locus_tag="VF958_08145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015352946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate kinase"
                     /protein_id="HEX9149114.1"
                     /translation="MKPTLSPEDLQIVLSELAEANQIFARRYPGDSPRRQPVHTVYGG
                     AQVFRSDTAPRLGALARRSLHEYAVAPEEFGRVLGLPEPLAATIHERVLAKLEREPVE
                     DFRIDFEDGYGNRPDAEEDGHAASTADEVAKGLDAGTLPPHVGIRIKPFSEELKLRAI
                     RTLDIFLTRLVRATGGRLPDNFVVTLPKVIAPDQVTALASLLEQLERHLGLSERELLL
                     EIMIETPQSVIDAAGSAALPALVAAGRGRCLAAHFGTYDYTASLNVTATYQTMTHPAC
                     DFAKGVLQVALAGTGVWISDGATNVLPVPPHRAAAGGPPLSPRQIADNRTAVHAAWRL
                     HYEHVRHSLEGGFYQGWDLHPAQLPTRYAALYAFFLEGIDGASARLKNFVAKAGQATL
                     VGEMFDDAATGQGLLNHFLRAWNCGAITEDEAVEKSGLTSDELRRRSFVTILKSRRS"
     gene            complement(1549..3603)
                     /locus_tag="VF958_08150"
     CDS             complement(1549..3603)
                     /locus_tag="VF958_08150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012934760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX9149115.1"
                     /translation="MESFLLSSPVETSARAATSKPEVLPPFGPRERRFAVFAGLMLVL
                     FLAALDSAIVATALPTIVGELGGLAHLSWVVTAYLVGNTIVTPLYGKLGDLYGRKRVL
                     QVAALIFLAGSALCGFARTLTELILFRFLQGLGGGGIIVSTQASLADIVPPRERGRYQ
                     GIFGAVFGVSSIAGPLLGGFFTTHLSWRWIFYVNLPVGALALVVLAITLPATAERVRR
                     RIDYAGSALLAGALTAIVLLADLGGTAAPWGSPLVIGLIVAAVALLVVFVRVERQSSE
                     PVVPLHLFRNRVFNVTSAIGFIVGFALFGSITYLPLFLQLVKGSSPTVSGLELVPMMG
                     GMLFMSILAGQVVSHVGRYRMFPIVGTAVLTAGLYLLSRMDSTTSRLTSSLFMLVVGM
                     GLGMVMQVLVLAVQNAVEYEDLGVATSGATLFRLIGGSFGVAVLGAIFASRLETALSH
                     AVLSRAVDLGHLAPGTLLRLPPATRNAYLAAFSGSLRTVFLVAAAVALVSFLFSWFLE
                     DRPLRETVAASGAGLGEGFAMPSGSDSLTQISHGLSLLMHRDARHRILEQITARAGVD
                     LDPEASWLLSQIERCPEMEIERLAHEFGVDPDRLGPAWRELENRGLIVAGAAPCPTLT
                     AAGREVSERLMKARRERLGELLADWSPEQHRQVAEILRKYAGDVEGEELSDPRTGGSS
                     GS"
     gene            complement(3700..3999)
                     /locus_tag="VF958_08155"
     CDS             complement(3700..3999)
                     /locus_tag="VF958_08155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9149116.1"
                     /translation="MVNFKISLTKSPQGEILVNGNYVSGNDPVVVERVNVVELDSAGN
                     TIGATTYRLNKSIDPSPGNYLLVSQAPSGSNVKAARATAYYYLIDKTAQSTTLNL"
     gene            complement(4009..>4375)
                     /locus_tag="VF958_08160"
     CDS             complement(4009..>4375)
                     /locus_tag="VF958_08160"
                     /inference="COORDINATES: protein motif:HMM:NF013201.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="urate oxidase"
                     /protein_id="HEX9149117.1"
                     /translation="YTTLKETNDRILATSLTTVWTHAAAAPRTAWDAVRKTLLDAFAA
                     HRSASVQHTMYAMGEAVLAKHESLSQIRMTMPNKHHLLVDLTPFGLENKNEIFVATEE
                     PYGLIEATVTRNRSNSVKK"
BASE COUNT          769 a         1477 c         1474 g          655 t
ORIGIN      
        1 atggccgcgg tgcgctcgga tcgtccgcgc atcgcgctcg gcagcgtggc gccgaccgtc
       61 gtgcggctgc ccgagaccga agcggcgctc gcggcgggtg ccccgatcgc ggaggcgcgt
      121 cgtgtgctcg aggtcgaaat ccggccgatc gacgatctcc gctccaccgc ggagtaccgg
      181 cggcgggtgt cgggtaacct gctggagcag ttctggaggg agacctcatg aagcccacgc
      241 tttctcccga agacctccag atcgtcctct ccgagctcgc ggaggccaac cagatctttg
      301 cgcgccgata ccccggcgac tctccccgcc gccagccggt ccacaccgta tacggcggtg
      361 cccaggtctt ccggtccgac acggcgccca ggctcggcgc cctcgcgcgg cggtcgcttc
      421 acgagtacgc ggtggcgccg gaagaattcg gacgggtgct cgggcttccc gaacctctcg
      481 ccgcgacgat tcacgagcgt gtcctcgcga agctcgagcg cgagccggtc gaagacttcc
      541 ggatcgactt cgaagacgga tatggcaatc gcccggacgc cgaggaggac ggccacgcgg
      601 catccaccgc ggacgaggtc gcgaaggggt tggacgcagg aacgctcccg ccccacgtcg
      661 gaatccgtat caagccgttc agcgaggaat tgaagctccg cgcgatccga acgctcgaca
      721 tcttcctcac ccgcctcgtt cgcgcgaccg gcggccgtct gccggacaac ttcgtcgtga
      781 cgctccccaa agtgatcgcc cccgatcagg tcaccgcgct cgcgtccctg ctcgagcagc
      841 tcgagcggca cctcgggctc tccgagcgcg aactcctcct cgagatcatg atcgagaccc
      901 ctcaatcggt gatcgatgcg gccggcagcg ccgcactccc ggccctcgtc gccgccgggc
      961 gagggcgctg cctcgctgcg cacttcggca cgtacgacta caccgcgtca ttgaacgtta
     1021 cggcgacgta ccagaccatg acgcatcccg cctgcgactt cgccaaagga gtcctgcagg
     1081 tcgcgctcgc cgggaccggc gtatggatct cggacggcgc gacgaacgtc cttcccgtgc
     1141 cgccgcaccg cgccgcggcg ggcggacctc ctctctcgcc gcggcagatc gccgacaacc
     1201 ggacggccgt tcacgccgcg tggagacttc actacgagca cgtccggcac tcgctcgagg
     1261 gcggcttcta ccaggggtgg gacctgcacc cggcgcagct ccccacgcgt tacgccgcgc
     1321 tctacgcgtt cttcctcgag ggaatcgacg gcgcatcggc gaggctcaag aacttcgtcg
     1381 cgaaggccgg acaggcgacg ctcgtgggcg agatgttcga cgacgccgcg accgggcagg
     1441 gactcctgaa ccacttcctc cgcgcgtgga actgcggcgc gatcaccgag gacgaggcgg
     1501 tcgaaaagag cggcctgacg tcggacgagc tccgccggcg ctcgttcgtc acgatcctga
     1561 agagccgccg gtcctagggt cggagagctc ctccccttcg acgtcgcccg cgtacttgcg
     1621 gaggatctcc gcaacctggc ggtgctgttc cggcgaccag tcggccagga gctcgcccag
     1681 ccgttcgcgg cgggccttca tcagcctctc gctgacctcc cgaccggccg ccgtgagcgt
     1741 cgggcaggga gccgcgcccg cgacgatcaa gccgcgattc tccagctcgc gccacgccgg
     1801 gccgagccgg tcgggatcga ctccgaactc gtgcgcgagc cgctcgatct ccatttcggg
     1861 gcaacgttcg atctgcgaga gaagccagct cgcctcggga tcgaggtcga cgccggcgcg
     1921 cgccgtgatc tgctcgagga tccgatgccg cgcgtcgcgg tgcatcagca gggaaagccc
     1981 atggctgatc tgagtgagcg agtcggagcc cgagggcatc gcgaaaccct ctccgaggcc
     2041 ggcgccgctc gcagccacgg tctcccggag cggccgatcc tcgagaaacc aggagaagag
     2101 gaagctcacg agagcgaccg ccgcggcgac gagaaaaacc gtccggaggg aaccggaaaa
     2161 ggccgcgagg tacgcgttcc gggtcgccgg agggagacgg agaagcgtcc ccggagcgag
     2221 gtgtccgagg tcgacggcgc ggctgagaac ggcgtgggag agcgccgttt cgagacgcga
     2281 ggcgaagatt gcgccgagga cggcgacgcc gaaagacccg ccgatcagac ggaagagcgt
     2341 cgccccggag gtcgcgacgc cgaggtcctc gtactcgacc gcgttctgga cggccaggac
     2401 gaggacctgc atgaccattc ccagccccat gccgacgacg agcatgaaga gggacgacgt
     2461 gagccggctc gtcgtcgagt ccattcggga gaggagatag agcccggcgg tgaggacggc
     2521 ggtgccgacg atcggaaaca tccggtagcg cccgacgtgg ctcaccacct gccccgcgag
     2581 aatggacatg aatagcattc cgcccatcat cggcacgagc tcgaggccgg agaccgtggg
     2641 actcgatccc ttgacgagct gcaggaagag cgggagatag gtgatcgatc caaagagcgc
     2701 gaagccgacg atgaagccga tcgcgctcgt cacgttgaac acccggttgc gaaagagatg
     2761 caacggcacc acgggctcgg agctttgccg ctcgacgcgc acgaacacga cgagaagcgc
     2821 cacggcggcg acgatcaagc cgatcacgag cggcgagccc cacggcgccg ccgtcccccc
     2881 gagatccgcc agcagcacga tcgccgtcaa cgctcccgcg agaagagcgg agccggcgta
     2941 gtcaatccgg cggcggacgc gctccgccgt cgcggggagc gtgatggcga gaacgacgag
     3001 cgcgagcgcg ccgaccggga ggttgacgta gaagatccag cgccacgaga ggtgcgtcgt
     3061 gaagaacccg cccaggagag gtcctgcgat cgacgacacg ccgaacaccg cgccgaagat
     3121 cccctggtag cggccgcgtt cgcggggcgg aacgatgtcg gcgagcgacg cctgcgtgct
     3181 gacgatgatg ccgcctccgc cgagtccctg aaggaaccgg aagagaatga gctcggtcag
     3241 cgtccgtgcg aagccgcaga gagcggagcc cgcgaggaaa atgagcgcgg caacctgcag
     3301 cactctcttt cggccgtaca ggtcgccgag ttttccgtag agcggcgtga cgatcgtatt
     3361 accgaccagg taggccgtca ctacccagga gaggtgagcg aggccgccga gctcgccgac
     3421 gatcgtcggc agcgccgtcg ccacgatcgc ggagtcgagc gccgcgagaa acaggacgag
     3481 catcagcccc gcgaagaccg cgaagcggcg ctcgcgggga ccgaagggcg ggaggacctc
     3541 gggcttcgag gtcgctgcac gagcgctcgt ctcgaccgga ctgctcagga ggaagctctc
     3601 cacgcgaacg cgcgcgctgc aagagcgaag ccgcttccgg agtgcgcggt gtcggtaatg
     3661 tcccgaaaga tcggctcggg ggatccttct ttcaccccgt cagaggttga gcgtcgtcga
     3721 ctgcgccgtc ttgtcgatca ggtagtagta ggccgtggcc cgggccgcct tgacgttgct
     3781 tccggacggc gcctgcgaga ccaggaggta gttgccgggg gacgggtcga tgctcttgtt
     3841 caatcggtac gtcgtcgcac cgatcgtatt ccccgcgctg tcgagctcca cgacgttgac
     3901 tcgctcgacg acgaccggat cgttgccgct gacgtagttt ccgttcacga gtatttcccc
     3961 ctggggactc ttggtcagag atatcttgaa attgaccatc gctcctcctt atttcttcac
     4021 tgagttggaa cggttgcgcg tcaccgtcgc ttcgatcaac ccgtagggct cctcggtcgc
     4081 gacgaagatt tcgttcttgt tctcgaggcc gaacggcgtg aggtccacga gcagatggtg
     4141 cttgttcggc atcgtcatgc ggatctgcga aagactttcg tgcttcgcga ggaccgcctc
     4201 tcccatcgcg tacatcgtgt gctgtaccga ggcgctccgg tgcgccgcga aggcgtcgag
     4261 gagcgtcttc cgcacggcat cccacgccgt ccgaggcgcg gccgccgcgt gcgtccacac
     4321 cgtcgtcaac gaggtcgcca ggatgcggtc gttcgtctcc ttcaacgtcg tgtag
//
LOCUS       DASWAV010000393         2196 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U2665
            k141_21946, whole genome shotgun sequence.
ACCESSION   DASWAV010000393 DASWAV010000000
VERSION     DASWAV010000393.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960316
            Sequence Read Archive: SRR10260007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2196)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2196)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:47:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,929
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2196
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_21946"
                     /isolate="SMAG_U2665"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.36444 N 132.31889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..180)
                     /locus_tag="VF958_08165"
     CDS             complement(<1..180)
                     /locus_tag="VF958_08165"
                     /inference="COORDINATES: protein motif:HMM:NF012787.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin domain-containing protein"
                     /protein_id="HEX9149118.1"
                     /translation="MLAAITLAAATIIGVPAPDWGELTWVQGGPLSLSDLRGKVVLVR
                     FFTDPECPFCSATAPA"
     gene            complement(216..959)
                     /locus_tag="VF958_08170"
     CDS             complement(216..959)
                     /locus_tag="VF958_08170"
                     /inference="COORDINATES: protein motif:HMM:NF012958.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHB depolymerase family esterase"
                     /protein_id="HEX9149119.1"
                     /translation="MPDSRAVVLLLSTAFGCGCAPASTPVRALPRRQTAQEHAGVRYL
                     LHLPAAYESRPDWPVILFLHGAGERGRDLGLLLREGLPRILESLPDFPFVVVSPQEEK
                     GRLWTPDALAELVDDVVARERVDPERVYATGLSTGATAALEVAIRRPGKIAAVAAVSP
                     TRIPAALCGMKPVPTWIFQNAGDERVPATRATRLARELRRCGNAEVIVTLYPREGHDA
                     WTETYRRRDLYEWMLRHRSSPSPSGRGPG"
     gene            975..1403
                     /locus_tag="VF958_08175"
     CDS             975..1403
                     /locus_tag="VF958_08175"
                     /inference="COORDINATES: protein motif:HMM:NF020917.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDGSH iron-sulfur domain-containing protein"
                     /protein_id="HEX9149120.1"
                     /translation="MTEPRGTITIRPNGPYRVEGEVPLEGPDGTPYESKPAYSLCRCG
                     RSANKPYCDTTHKECGFVGTETADSPSEAPPLPGKAVEAAVIRIVRDGPYEVIGLSAL
                     AFSDGRVVQTRPYYRLCRCGASKTKPFCDDTHLKIGFRQG"
     gene            1433..>2196
                     /locus_tag="VF958_08180"
     CDS             1433..>2196
                     /locus_tag="VF958_08180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9149121.1"
                     /translation="MRLRVLIAAALALATGASAAEKKPLTVDDMWAVQRVGSPALSPD
                     GKTVAYTVSVYDLEENRSNGDVWIVPLAGGTPRRLTTNKASDGSPAWSPDGRRIAFTS
                     KRDDDKANQLYVIAVDGGEPERVTERPLAVSNPKWLPDGKRIAFVSHVIAGAESPADT
                     KKALDAREKSKVKARVTENRLFRFWDRWLTDDEFPHIFVVDVGTKKVTDLLPGSKRLF
                     SLQEGSGSFDVSPDGAWIAFEANSTPEPYEKLNRDV"
BASE COUNT          390 a          737 c          755 g          314 t
ORIGIN      
        1 ggcgggggcg gtcgcgctgc agaacgggca ctcgggatcc gtgaagaacc gcacgagcac
       61 gaccttgccg cgcaggtcgg agagcgacag gggaccaccc tgcacccagg tgagctcgcc
      121 ccagtcgggc gcgggcacac cgatgatcgt ggcagcggcg agcgtgatgg cggcgagcac
      181 ggccggattc taggtcctcg ccgaaatcaa tcccctcaac ccggccctct cccggagggc
      241 gagggagaag atcggtggcg cagcatccac tcgtacaggt cccgccgccg gtacgtttcc
      301 gtccacgcat cgtgaccctc gcggggatag agggtgacga tcacctccgc gttcccgcac
      361 cgccggagct cccgcgcgag cctggtcgcg cgcgtcgctg gaacgcgctc gtcgccggcg
      421 ttctgaaaga tccaggtcgg gaccggcttc atgccgcaga gcgccgccgg tatccgcgtc
      481 ggcgagaccg cagcgaccgc cgcgatcttc ccgggccgac ggatcgcgac ctcgagcgca
      541 gcggtggctc cggtcgagag gccggtcgcg tagacgcgct cgggatccac gcgttcgcgc
      601 gccacgacgt cgtcgacgag ctccgccagc gcgtcgggcg tccagagccg tcccttctcc
      661 tcctgcggcg atacgacgac gaacggaaag tccggaagcg actcgagaat tcgcgggagc
      721 ccttcgcgca ggaggagacc gaggtcccgg ccgcgctctc ccgcgccgtg gagaaacagg
      781 atgacgggcc aatccggacg actctcgtac gccgcgggga ggtggagcag gtagcgaacg
      841 ccggcgtgct cctgcgcggt ctggcgtctc ggcaacgcgc ggacaggggt cgatgccggg
      901 gcgcacccgc agccgaaagc cgtcgacaga aggagaacga ccgcgcgcga atctggcatg
      961 attcgggccc gatcatgact gaaccccgag ggaccattac gatccggccg aacggtccct
     1021 accgcgtgga gggggaagtc ccgctcgagg ggcccgacgg gacgccgtac gagtcgaaac
     1081 ccgcttattc gctctgccgc tgcggcaggt ccgcaaacaa gccttactgc gacacgacgc
     1141 acaaggagtg cggtttcgtc gggacggaga cggccgactc cccatcggag gccccgcctc
     1201 tccccgggaa agcggtcgag gcggcggtga tccggatcgt gcgggacggc ccgtacgaag
     1261 tcatcggact ctcggccctg gcgttctcgg acggccgggt agtccagact cgaccctact
     1321 atcggctttg ccgctgcggc gcgtcaaaga ccaagccctt ctgcgacgac acacacttga
     1381 aaatcggttt tcggcagggt tgacgaccga cgcccggccg gaaggagtga ggatgcgact
     1441 tcgtgttctg atcgcggccg cgctcgccct cgccacgggc gcttccgccg cggaaaagaa
     1501 gccgctcacc gtcgacgaca tgtgggcggt gcagcgggtc ggctcgccgg cgctctcgcc
     1561 ggacggaaag accgtcgcct acaccgtctc ggtctacgac ctggaggaga accgttccaa
     1621 cggagacgta tggatcgttc cgctcgccgg ggggacgccg cgaagactca cgaccaacaa
     1681 agcctccgac ggctcgcctg cctggagccc ggatgggaga cgcattgcgt tcacgtcgaa
     1741 gcgtgacgac gacaaggcga accagctgta cgtgatcgcg gtcgacggcg gggagccgga
     1801 acgcgtcacc gagaggccgc tcgccgtctc gaacccgaag tggcttcccg acgggaagcg
     1861 catcgcgttc gtctcgcacg tgatcgcggg cgccgagtcg ccggctgata cgaagaaggc
     1921 gctcgacgct cgcgagaaaa gcaaggtgaa agcgcgcgtc accgagaacc gcctcttccg
     1981 cttctgggac cgttggctga cggacgacga gtttccgcac atcttcgtcg tggacgtcgg
     2041 cacgaagaaa gtcaccgatc tcctgccggg ctcgaagcgc ctgttcagcc tccaggaggg
     2101 gtcggggagc ttcgacgtgt cgcccgatgg tgcctggatc gctttcgaag cgaactcgac
     2161 gcccgagccc tacgagaagc tgaaccgtga cgtctt
//
LOCUS       DASWAV010000394         6850 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U2665
            k141_780329, whole genome shotgun sequence.
ACCESSION   DASWAV010000394 DASWAV010000000
VERSION     DASWAV010000394.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960316
            Sequence Read Archive: SRR10260007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 6850)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6850)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:47:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,929
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6850
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_780329"
                     /isolate="SMAG_U2665"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.36444 N 132.31889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            127..1155
                     /locus_tag="VF958_08185"
     CDS             127..1155
                     /locus_tag="VF958_08185"
                     /inference="COORDINATES: protein motif:HMM:NF012770.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEX9149122.1"
                     /translation="MSERTGSRQPRRRILGVAFCLGFAATVIRAAPRARTGTVSNGNV
                     RLAATLDLPDGPGPHPVMVVLHASGEGERGFASYRHLPKILPASGIGVLRYDRRGSGA
                     SSGDFERASFPDLASDARAVIAWARASADVDRRRLGLWALSQGGWIAPLVAAADPSIA
                     CLVIVSGAGGTPAEQMLYSARFALREAGYDSRAIERAVRLRRAVDAYYAGRRPREDVA
                     TILKEEKSEPWYKLAAVPDELPRDVRSTKWYYQFAFDPAASMAKVRAPVLLLHAERDP
                     WIPIEQARFAWKARSPAPLTIRTIPGANHFMAATTDPAHDADPEPISEEYTRAMVEWL
                     TNALGIRR"
     gene            1311..1847
                     /locus_tag="VF958_08190"
     CDS             1311..1847
                     /locus_tag="VF958_08190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008449206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030638 - polyketide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ester cyclase"
                     /protein_id="HEX9149123.1"
                     /translation="MTYLRTALSIAVASFLLMAGGSAAAPAPPNRDAEKQAVSAHIAN
                     FDDLDFNVFSNQKWGELSRSHSKDIVVHWPDGHQTRGIAKHIEDLKAMFVYAPDTRIK
                     VHPVKFGSGDWTSAISVMEGTFSKPMPGPDGKLIPPTGKAFKLEMCTVGHWKGGVMDE
                     EYLFWDNLTFMKQIGLVK"
     gene            1883..2353
                     /locus_tag="VF958_08195"
     CDS             1883..2353
                     /locus_tag="VF958_08195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009714116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX9149124.1"
                     /translation="MNNSDIRLRDVHPNDLPVFFEQQLDLDAIRMAAFPARDKDAFAA
                     HWEDVLSDGAVTTKTILFEGRVAGYIVSFERSGRPLVGYWLGKGYWGRGIATRALSQF
                     LDVVAMRPLYAHVARHNVASIRVLEKCGFRDSGEEIGPAASDEVEEIIMVLGAS"
     gene            2350..2895
                     /locus_tag="VF958_08200"
     CDS             2350..2895
                     /locus_tag="VF958_08200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016086899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX9149125.1"
                     /translation="MNVLETERLFLRKLTLEDAPFILELVNDPSWLRFIGDKGVRNLE
                     DARDYIRNGPMDMYARLGFGMYLVERKSDRAAVGICGLTKRDFLDRPDVGFALLPQFR
                     LQGYARESAAAVVEHAWKTLGLDRLLAITSPENVVSIRLLDKLGFVFQRMLRQSPTDR
                     VSLFEAANPSPGSERRRKSAR"
     gene            2892..3233
                     /locus_tag="VF958_08205"
     CDS             2892..3233
                     /locus_tag="VF958_08205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9149126.1"
                     /translation="MSRTTLFDLVQQVDELDDRDRSRPLVIYAQNGADAMRSSPALVC
                     PRGEDESPACPLDSSLSEVLSVEQAREAIRIWSEWRGGVTPTSEERFRTVMFYSQNGA
                     FVPLETDREGM"
     gene            complement(3274..4149)
                     /locus_tag="VF958_08210"
     CDS             complement(3274..4149)
                     /locus_tag="VF958_08210"
                     /inference="COORDINATES: protein motif:HMM:TIGR02177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:ferredoxin oxidoreductase subunit
                     beta"
                     /protein_id="HEX9149127.1"
                     /translation="MSEQQLPAFQAADYKSDLKPVWCAGCGDFGALAALHRAMAKLQL
                     EPWNTVVVSGIGCSSRLPGYVSTYGFNGVHGRALTLATGMKVARPDLTVVAVGGDGDG
                     IAIGGNHFLHTARRNLDVAYFIMDNEIYGLTKGQVAPTTPAGEKTKSTYWGNPEPSVD
                     PCELAISFGATWVGRGFSGDPKSLVELMTQAIEHHGFAFLNILSPCVTWRGDDQFKTL
                     KAKQRFLPADHDRRDRAAALKYTREVDVMTTGVLYEIEQPSFIDQQKEIRARAQEAGV
                     ISTAAQILERLAPAF"
     gene            complement(4146..5957)
                     /locus_tag="VF958_08215"
     CDS             complement(4146..5957)
                     /locus_tag="VF958_08215"
                     /inference="COORDINATES: protein motif:HMM:TIGR03710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase subunit alpha"
                     /protein_id="HEX9149128.1"
                     /translation="MSTNDLTIAMVGSGGDGVVTMGDLIAMVAAREGLHVIKTEGYGP
                     QIRGGESSCTVRVAARDLFAQGDAVDVLLVFRWSDFARFRGEIEVARDAVVLYEATDT
                     TPVEELELPVEDRARWIPVPFDRLSREATGAAGSKNVVALGVLVGLFGLPVDTMRRAI
                     HQRFGRKKESVREANAKAFEAGLAFASTLGEDLADKRLAYVPGPTRLLLSGNEAAALG
                     ALHAGCRFFAGYPITPSSEILHFLAEWMPRAGGSVVQTEDELAAIGAIIGASFAGAKA
                     MTATSGPGLSLMTEMLGLASMAEIPIVIIDVQRGGPSTGNPTKGEQSDLFQCLYGTHG
                     DAPRVVLACSDVEDCFHATVEAFNIAEEFQLPVLVLSDQAVGQRKETLSAETLKHEVH
                     DRLIPAPGELENYERYRDTPSGVSPMSVPGLKNGMYQTNGLEHDEAGRPNSLYLTHEK
                     MNEKRYRKLWPIRDRYNFVRRYGPAEADLGVLCWGSSKGPVQEAVLAANARGKKVAAF
                     VPQVLYPFPKREFEEFLAQVGELLIVELSYTAQFYKYLRTFLNLPEERTHVFKRSGGK
                     NLTVAEVDREIQKVLSALPARGMKPAVSAARGMEVIQ"
     gene            6121..6567
                     /locus_tag="VF958_08220"
     CDS             6121..6567
                     /locus_tag="VF958_08220"
                     /EC_number="2.1.1.100"
                     /EC_number="2.1.1.334"
                     /inference="COORDINATES: protein motif:HMM:NF016107.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenylcysteine carboxylmethyltransferase
                     family protein"
                     /protein_id="HEX9149129.1"
                     /translation="MRPASLKALSLAGLLLMLVAMLGLLASNSLLARNAVGGAVQVAA
                     FGLMVWARLTFGSRSFHASADPTPGGLVTTGPYRFVRHPIYTAICLFGWAGVLSNWSG
                     LAAALGVLLIAGAIARMVCEERLVVEKYPEYRDYATKTKRMVPHVF"
BASE COUNT         1167 a         2222 c         2298 g         1163 t
ORIGIN      
        1 accgggattt ccggatcgcg ccgccgaagg attttccgtg gggccgcgaa gtccacttca
       61 tcgacccggc gggagtgtgc tggcacgtcg gagcggggcg atcgtgacga ctcgcgcgcg
      121 aaccccttga gtgagcggac cggctcgcgg caaccgcgcc ggcggattct cggagtcgcc
      181 ttctgtctcg gcttcgctgc gaccgtgatc cgcgcggcgc cccgcgcccg gaccggcacc
      241 gtgtcgaacg gcaacgtccg gctcgcggcg actctcgatc tcccggacgg accgggtccg
      301 catcccgtca tggtcgtcct gcacgcgtcg ggggaggggg agcgggggtt cgcgtcctac
      361 cggcatctcc cgaagatcct tccggccagt ggaatcggcg tgcttcgcta cgatcgacgg
      421 ggttccggcg cctcgtcggg agatttcgaa cgggcgagct tccccgatct cgcgtcggat
      481 gcccgggcgg tcatcgcgtg ggcgcgcgcc tccgccgacg tcgaccggcg acgcttgggg
      541 ctctgggcat tgagccaggg gggctggatc gcgcctctcg tcgcggcggc cgatccatcg
      601 atcgcttgtc tcgtaatcgt gtcgggcgcc ggagggaccc ccgcggagca gatgctgtac
      661 agcgcccgtt tcgcgttgcg cgaagcgggc tacgactcca gggccatcga gcgggcggtc
      721 cgcctgcgtc gagccgtcga tgcttactac gccggaaggc gtccgcgcga agatgtcgca
      781 accatcctga aggaggagaa gtcggagccc tggtacaagc tcgccgccgt tcccgacgag
      841 ctcccgcgcg acgtccggag caccaagtgg tactaccagt tcgccttcga tccggcggcg
      901 agcatggcga aagtccgggc gccggtcctg ctcctgcatg cggagcgaga tccctggatt
      961 ccgatcgagc aggcccgatt tgcctggaag gcgcgttctc ccgcgccgct gacgatccgg
     1021 acgattcccg gagcgaacca tttcatggct gcgacgacgg acccggcgca cgacgcggat
     1081 cccgagccca tctccgagga atacacgcgg gcgatggtgg agtggctcac gaacgcgctc
     1141 ggcatccggc gatgacggcg tgagacaggt ccggccggag accgaccgca cccgcagaat
     1201 agaatcactc gcgatatcga gcgcgagtcc gcccgcactc gcggaaagag caatggagag
     1261 cgacgaacaa ctctgtgagg cggaccagcg ccccgaaagg agatcgacca atgacgtacc
     1321 ttcgcacggc tctgtccatc gcagtggcaa gcttcctcct gatggcgggc gggagcgccg
     1381 ccgctccggc gcctccgaat cgcgatgccg aaaagcaggc cgtgagcgcc catatcgcga
     1441 atttcgacga cctggacttc aacgtcttct cgaatcagaa gtggggggag ttgagccgga
     1501 gccactccaa agacatcgtc gtccactggc ccgacggcca tcagacccgc gggatcgcca
     1561 aacacatcga ggacctcaag gcgatgttcg tctatgctcc ggacacacgg atcaaggtgc
     1621 atcccgtcaa gttcggctcg ggcgactgga cgagcgcgat cagcgtgatg gagggaacgt
     1681 tctcgaagcc catgccgggg ccggacggga agctgattcc gcccaccggc aaggcattca
     1741 agctcgagat gtgcaccgtc ggtcactgga agggcggcgt gatggacgag gaatacttgt
     1801 tctgggacaa cctgacgttc atgaaacaga tcgggcttgt gaagtagcga gcccgtccgg
     1861 tgcgggggcc ggagctcgcg cgatgaacaa cagcgacatc cggctgcggg acgtccatcc
     1921 gaacgacctg cccgtgtttt tcgagcagca actcgatctc gacgccatcc ggatggccgc
     1981 gttcccggcg agagacaaag acgcgttcgc ggcgcactgg gaggacgttc tctccgacgg
     2041 cgccgtcacg acgaagacga tactgttcga gggtcgcgtg gccggttaca tcgtgagctt
     2101 cgagcggtcg ggccgaccgc tggtcggcta ctggctcggg aagggctact ggggcagggg
     2161 gatcgccacc cgggcgctgt cgcagttcct cgacgtcgtc gcgatgaggc ctctctacgc
     2221 ccacgtggcc cggcacaacg tcgcctccat ccgcgtgctg gagaagtgcg gcttccggga
     2281 ctccggcgag gagatcgggc ctgccgcctc cgacgaagtc gaggagatca tcatggttct
     2341 cggcgcgtca tgaacgttct cgaaaccgag cggctcttcc tccggaaact cactctggaa
     2401 gacgccccct tcatcctcga gctcgtgaac gatccttcgt ggctgcgatt catcggggac
     2461 aagggagtgc gaaacctcga ggacgcgcgc gactacatcc gcaacggtcc gatggacatg
     2521 tacgcccgcc tcgggttcgg gatgtacctc gtcgagcgaa agagcgaccg cgcggcagtc
     2581 gggatctgcg gcctcaccaa acgcgatttc ctggatcgcc ccgacgttgg cttcgccctt
     2641 ctcccgcagt ttcggctgca gggttacgcg cgggaatccg cggcggcggt cgtggagcac
     2701 gcctggaaga ctctcgggtt ggaccgtctt ctcgccatta cctcgccgga gaacgtcgtg
     2761 tcgatccggc tgctcgacaa gctcggtttc gtgttccagc ggatgcttcg gcagtcgccc
     2821 accgaccggg tgagcctctt cgaagcggcg aacccatccc cgggctccga gcgacgccgg
     2881 aaatcggcgc gatgagccgc acgactcttt tcgacctcgt ccagcaagtg gacgagctcg
     2941 acgatcgcga ccgttctcgt cccctcgtga tttacgcgca gaacggtgcc gatgcgatgc
     3001 gaagcagtcc ggccctcgtc tgtccgcgcg gcgaggacga gagtccggcg tgtcccctcg
     3061 acagttccct ttccgaagtt ctcagcgtcg agcaggcgcg cgaggcgatc cggatatggt
     3121 cggagtggcg cggcggagtc acgccgacgt ccgaagagcg attccggacg gtcatgtttt
     3181 actcgcagaa cggcgccttc gttccgctcg aaacggatcg cgagggcatg tagagagcgg
     3241 gactcccgga ggaggagtcc cgctgagggt cgcctagaaa gccggggcga gcctttcgag
     3301 gatctgcgcc gcggtcgaga tgacgcccgc ctcctgggcc cgtgcgcgga tctctttctg
     3361 ctggtcgatg aaggacggct gttcgatctc gtagagcacg ccggtcgtca tgacatcgac
     3421 ctcgcgcgtg tacttgagcg ctgccgcgcg atcccgccga tcgtggtcgg ccgggaggaa
     3481 tcgctgtttc gccttgagcg tcttgaactg atcgtctcca cgccaggtga cgcacgggga
     3541 gaggatgttc aggaacgcga agccgtggtg ctcgatcgcc tgagtcatca gctcgaccag
     3601 gctcttcgga tcgcccgaga agccgcgccc gacccacgtc gctccgaacg agatcgcgag
     3661 ctcacacgga tcgactgagg gctccggatt gccccagtag gtcgacttgg tcttctcgcc
     3721 ggcgggcgtc gtcggagcga cctggccctt cgtcagcccg tagatctcgt tgtccatgat
     3781 gaagtacgcc acgtcgaggt tgcgccgcgc ggtgtggagg aaatggttgc ctccgatcgc
     3841 gatgccgtcc ccgtctccgc cgacggcgac gaccgtcaga tccggccgcg cgaccttcat
     3901 gcccgtcgcg agggtgagag ctcggccgtg gacaccgttg aagccgtagg tcgagacgta
     3961 accgggcagc cgggaagaac agccgatgcc ggagaccacg acggtgttcc acggctccag
     4021 ttgcagtttc gccatcgccc ggtggagggc cgcgagcgcc ccgaagtccc cgcagccggc
     4081 gcaccagacg ggtttcaggt ccgacttgta gtcggcggcc tggaaggcgg gaagctgctg
     4141 ctcgctcact ggatcacctc catcccgcgc gccgccgata ccgcgggttt catcccgcgc
     4201 gcgggcaatg ccgaaagaac cttctggatc tcgcgatcca cctcggcaac ggtcaggttc
     4261 ttgccccccg accgcttgaa gacgtgcgtg cgttcttcgg ggagattcaa gaaggtccgc
     4321 aggtacttgt agaactgggc ggtgtaggag agctcgacga tcaggagctc gccgacctgc
     4381 gccaggaatt cctcgaactc ccgtttgggg aacggataga ggacctgcgg cacgaaggcg
     4441 gcgacctttt ttccccgcgc gttcgcggcg aggacggctt cctggacggg ccccttggag
     4501 gagccccagc acagtacgcc gagatccgcc tcggcagggc catagcgccg gacgaagttg
     4561 tatcggtcgc ggatgggcca gagcttgcga tagcgcttct cgttcatctt ctcgtgagtg
     4621 aggtagaggg agttcggccg gcccgcctca tcgtgctcga gcccgttggt ctggtacata
     4681 ccgttcttga ggcccggcac gctcatcggc gagactccgc tcggcgtgtc gcgatagcgc
     4741 tcgtagtttt ccagctcccc cggagcggga atcagccggt cgtgcacctc gtgcttcagc
     4801 gtctcggccg acagcgtctc tttgcgctgg ccgacggcct ggtcggagag gacgaggaca
     4861 gggagctgga actcctcggc gatgttgaag gcctccacgg tggcatggaa gcagtcctcg
     4921 acgtcggagc acgcgagcac cacgcgcggc gcgtccccgt gggttccgta caggcattga
     4981 aacaggtcgg actgctcgcc cttggtcggg tttccggtcg agggcccgcc ccgctgcacg
     5041 tcgatgatga cgatcgggat ctccgccatg gaagcgaggc cgagcatctc ggtcatgagg
     5101 gaaagacccg gtcccgaagt cgcggtcatc gccttggcgc ccgcgaagga cgcgccgatg
     5161 atggccccga tcgcggccag ctcgtcttcg gtctgcacca cggagcctcc cgcccggggc
     5221 atccactcgg ccaggaagtg gaggatctcg gaggaaggtg tgatcgggta acccgcaaag
     5281 aatcggcacc cggcatggag cgcccccagg gccgcggcct cgttgcccga gaggagcagc
     5341 cgggtcgggc cgggcacgta ggcgagacgc ttgtcggcga gatcctcgcc gagcgtgctt
     5401 gcgaaggcga gacccgcctc gaaagctttc gcgttcgcct cccgcaccga ctccttcttg
     5461 cggccgaacc gctgatggat cgcccggcgc atcgtgtcga cgggaaggcc gaaaagcccg
     5521 accaggaccc cgagcgccac gacgttcttg gagccggccg caccggtcgc ctcgcgcgaa
     5581 agccggtcga aaggaaccgg gatccagcgg gcgcggtcct ccacgggaag ctcgagctcc
     5641 tcgacgggcg tagtgtccgt ggcctcgtaa aggacgacgg cgtcccgagc cacttcgatc
     5701 tccccccgga agcgagcgaa gtccgaccag cgaaagacca ggagaacgtc gaccgcgtct
     5761 ccctgggcga agaggtcccg cgccgcgacg cggaccgtgc aggaagactc gcccccgcgg
     5821 atctgcggtc cgtagccctc ggtctttatg acgtggaggc cttcgcgcgc ggcaaccatg
     5881 gcgatcaggt cgcccatggt caccacgccg tcgcctccgg aaccgaccat tgctattgtc
     5941 aagtcgttcg tggacatgca gttttccttc cgccggcacc cgcgccggtc agcccgcagt
     6001 gtcccggacg cctccaggtg ccgcatgatg caaacaactc agtcccggaa ggcacgcccc
     6061 gagggccgaa aggtccgttc tgcgcggatc gcccgtcttc gagtggagga tgatcgtgaa
     6121 atgaggccgg cctcactgaa agccctctcc ctcgcgggtc ttctgctcat gctggtcgcg
     6181 atgctgggtc tcctcgccag caattcgctc ctcgcccgta acgccgtcgg gggcgcggtt
     6241 caggtggccg cgttcggcct gatggtgtgg gcgcgactga cgttcggcag ccgcagcttc
     6301 catgcctcgg cggatccgac gccgggcggg ctcgtcacca ccggtcccta ccgcttcgtt
     6361 cggcatccga tctacaccgc gatctgcctg ttcggctggg ccggtgtgtt gtcgaactgg
     6421 tccgggctcg cggcggcgct cggagtcctg ctgatcgccg gagcgatcgc ccgaatggtt
     6481 tgcgaggagc gactcgtcgt cgagaaatac ccggagtatc gggattacgc gacgaagacg
     6541 aaacgcatgg tgcctcacgt cttctgacga cgcgggcacc acgcgatctc ggccttcctg
     6601 gagcgcaggg tttatggcct gtgcgccagg tatgtgcggg gaagacgacg gaaggcgacg
     6661 agctccggac gcggagaact aacggcgcca gcggcgatag ccgaaaccac cgaagagaaa
     6721 aagtatgacc aggatgatga tgagagtcgt ggtcgacatg cagcgttccc tccggcaatc
     6781 tatacaggca cgttccatgc ctgactccgc agccaaacgc gcaaagggga gaaagggtag
     6841 agaggggaga
//
LOCUS       DASWAV010000395         9506 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U2665
            k141_182501, whole genome shotgun sequence.
ACCESSION   DASWAV010000395 DASWAV010000000
VERSION     DASWAV010000395.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960316
            Sequence Read Archive: SRR10260007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 9506)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9506)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:47:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,929
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9506
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_182501"
                     /isolate="SMAG_U2665"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.36444 N 132.31889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(147..563)
                     /gene="ndk"
                     /locus_tag="VF958_08225"
     CDS             complement(147..563)
                     /gene="ndk"
                     /locus_tag="VF958_08225"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013682217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004550 - nucleoside diphosphate kinase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HEX9149130.1"
                     /translation="MIERTLTIFKPDSVSAARSGALLARLEEEGFRVRGLKRLRLAPE
                     QARAFYAVHRERPFFEGLVRFMTEGPVVVAALERENAIAHLRQTMGATDSKKAEPGTL
                     RNLYGTDIERNAIHGSDSAENAAKEVSFFFSEAELI"
     gene            complement(564..986)
                     /locus_tag="VF958_08230"
     CDS             complement(564..986)
                     /locus_tag="VF958_08230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015324471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9149131.1"
                     /translation="MNGATLYHETQSFSPWAVALLVAVIGLIGALLSLRLTTTVTSDA
                     LAVKYGFLYKTRVPLTEIALAEAVEYAPVREYGGWGIRGTGRRRALNARGNLGVLLTR
                     ADGTTLLVGSQRPRELLEALAHAGVATQDRLPIVVKEF"
     gene            complement(983..1870)
                     /gene="sucD"
                     /locus_tag="VF958_08235"
     CDS             complement(983..1870)
                     /gene="sucD"
                     /locus_tag="VF958_08235"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010150335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate--CoA ligase subunit alpha"
                     /protein_id="HEX9149132.1"
                     /translation="MSIWVGRETRLLVQGLTGREGTFHTLASREYGTRVVAGVTPGKG
                     GTTHEEIPVFDTVARAVEATGANASMIFVPPPFAADAIVEAADAGIAVIVCITEGIPT
                     NDMLRVKKFLDEKNTSGAGVRLIGPNCPGIITPGECKIGIMPGRIHRPGTIGVVSRSG
                     TLTYEAVDQLTRLGLGQSTVIGIGGDPVNGTNFVDCLGAFGSDAGTEAVVLIGEIGGM
                     AEEEAACFVAGHFKKPVVAFIAGQTAPPGRRMGHAGAIIAGGRGTAAEKVRALEAASI
                     HVVPSPADIGARMKEVLGR"
     gene            complement(1867..2241)
                     /locus_tag="VF958_08240"
     CDS             complement(1867..2241)
                     /locus_tag="VF958_08240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9149133.1"
                     /translation="MKHVGGYALKSFGFFLGPIFVGLLVATWKGWPTLAANPRLLLGW
                     GVLLVFFFVLPLFLSRAGRAALGHEAERDSRRVWRTGAAALVVGAAAVLLIPSWRDLG
                     AWLSETLSELTGIPIVTEGRSR"
     gene            complement(2238..3410)
                     /gene="sucC"
                     /locus_tag="VF958_08245"
     CDS             complement(2238..3410)
                     /gene="sucC"
                     /locus_tag="VF958_08245"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015897678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-forming succinate--CoA ligase subunit beta"
                     /protein_id="HEX9149134.1"
                     /translation="MKVHEYQAKSILAKYGVAVPRGEVADSPAAAREVAQQIGGPVVV
                     KAQIHAGGRGKGGGIRVAQDAEQAFQHAKAILGMTLVTPQTGPEGRVVRKVLVEEALQ
                     TDRELYLGITLDRSRARPIVIASKSGGMEIEEVAARDPKAVVREPVDPALGMFPFQAR
                     RLAFDLGLAGDAFKQATALMQALFRAYLETDASLAEINPLLVTQAGRVLALDAKMNFD
                     DNALFRHPDIREMRDLAEEDPLEVEASKFGLNYIKLDGNVGCMVNGAGLAMATMDLVK
                     LAGGEPANFLDVGGGASEEQVKNAFRIILSDSNVRAILINIFGGIMRCDIIARGVVAA
                     VREMGLSLPVVVRLEGTNVDEGKRILSESGLTVTPAEGLADAARKAVGAALPRGRA"
     gene            complement(3481..4407)
                     /gene="mdh"
                     /locus_tag="VF958_08250"
     CDS             complement(3481..4407)
                     /gene="mdh"
                     /locus_tag="VF958_08250"
                     /EC_number="1.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012680498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HEX9149135.1"
                     /translation="MQQKVTVVGAGNVGAMVAQRLVEKEIADVVLIDIIEGIPQGKAL
                     DMMESAPVEKFDARMQGSNTYELSDGSDVVVVTAGLARKPGMSRDDLVQKNQEIVGAV
                     VREAARRSPKAVLIVVSNPLDAMCEVARRVSGFPRERVFGMAGILDSARMRWFLAEEL
                     DVSVESTHAFVLGGHGDTMVPLPRYSTVAGIPITQLLSKERIDAIVKRTRDGGIEIVN
                     YYKTGSAYYAPSAAVAEMVESVLKDEKKILPCAAYLEGEYGMSGVYLGVPVKLGRKGV
                     EKIMEIELTADEKSALGRSADSVRELFRILTV"
     gene            4488..4847
                     /locus_tag="VF958_08255"
     CDS             4488..4847
                     /locus_tag="VF958_08255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9149136.1"
                     /translation="MAERPDYKSHPLWNEAISLTHAAYDLAELVRPADPITARHLRRA
                     AVAIPAHVAGALTSDEEGRREHVLTARGALAEISRQAGRAEASADPATAAELARRAGA
                     LDRAVSLELGLAQGGIS"
     gene            4847..5233
                     /locus_tag="VF958_08260"
     CDS             4847..5233
                     /locus_tag="VF958_08260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9149137.1"
                     /translation="MPGERARPALYAVLAGALVLAMAAFWLPRWWENREYRQAEAIAG
                     VGARIVPGKIAEARKQLDPGVPGSRVTAALGPPSFSVKTEGASSHEIWIYYFQDGTLT
                     VNLTDGYVARAATEFGPPKIRKSRRP"
     gene            5250..5645
                     /locus_tag="VF958_08265"
     CDS             5250..5645
                     /locus_tag="VF958_08265"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013900248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HEX9149138.1"
                     /translation="MSDRDRAADPRREPSIRVVLMPKDTNAHGTIFGGVILSYIDQAA
                     AVEAKRHGATFIVTVAMREVVFHEPVYVGDLVSFYTGLVRIGRTSITVSVEVFAQRGE
                     GTAGMVKVTEAEVTFVNLDEERKPLPIPR"
     gene            complement(5805..5927)
                     /locus_tag="VF958_08270"
     CDS             complement(5805..5927)
                     /locus_tag="VF958_08270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9149139.1"
                     /translation="MAEEDVEDEPELEDLADEEDEVELPVEPGAAAEEEDDSEW"
     gene            5959..6351
                     /locus_tag="VF958_08275"
     CDS             5959..6351
                     /locus_tag="VF958_08275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9149140.1"
                     /translation="MISPPFQGDSGAGRRGAAVVGDPDERGVADRDGRDALRAGALDL
                     DVFFLAVRAFGRGFAAVRRVRFGTALRRAVPLRATEVFRAVRLPRVVLRFFDDARELL
                     RVLRTRPADDREVFREPPRPAFFVFLAT"
     gene            6478..7071
                     /locus_tag="VF958_08280"
     CDS             6478..7071
                     /locus_tag="VF958_08280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9149141.1"
                     /translation="MLITPRPGGLTIEIRPLVRSRAWRRRLVVGAIVIAAAALFGISR
                     LVPLWETGLRRASFGDLPLPLLVFLTLAVGISTPLTLIGLAALAFAEESLEVTADEVV
                     ISTTAFEKTRVQRISLPALDHWRETRAPLPPWWSWSVTRLAARSAGRLHPIAGGAGPR
                     EKRRIGLALVQATNRPLIGDFGRLIAAAPDPRSTNLE"
     gene            7077..8384
                     /locus_tag="VF958_08285"
     CDS             7077..8384
                     /locus_tag="VF958_08285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004514349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEX9149142.1"
                     /translation="MSGTRATPHGHLSETPLDSYDLPAEVRRGIADAGFVRATPIQEK
                     ALPIALAGRDVAGQAQTGTGKTAAFLITIFARLLRSHPKNPPRGPRSLVVAPTRELVV
                     QIDAEAKLLGVHTGLSTLAVFGGIDYRKQREALRQRPDLLVGTPGRLLDYEQQGATTF
                     RDVEVLVIDEADRMFDMGFIRDLRRILRRCPPYDARQTMLFSATLSPRVMELAYEHTN
                     NAEKVEIEPERVGAHGITEVLYHVSTREKLALLLGLLAREGGSRVLIFVNRRTTAVEL
                     VRALSANGYPTRALAGNVPQEKRLKILRDFKEGRLAVLVATDVASRGLHIEGVSHVVN
                     YDLPQDAEDYVHRIGRTGRAGALGTAVSIACEDYVFSLDAIQKLIGREIPVEWAPAAL
                     LEVGRNFVETKGPRRPTPARRPPPRRPPAEVTYGREKRKSRRR"
     gene            8453..>9506
                     /locus_tag="VF958_08290"
     CDS             8453..>9506
                     /locus_tag="VF958_08290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9149143.1"
                     /translation="MRSPRPSSVVLIAVLTVSTLFAAGGGRLRRERPAESRSRGVPRD
                     LALPAADGVSDPRRLERAIAQWNRLPRAFSPSSPALGSIGGTVWKPIGPNPIVEQGCC
                     GSTIIFAANGRVNSIAVDPTNSDVIYLGSAGGGVWKSVDQGAHWKPVTDQEVSLGIGS
                     SHAIAIDPNHPSTVYAGTSSWSLLNPGDRAQSRGILKSTDGGASWVVLGSGTPSGNVG
                     NAEDFFRDRNISTILVDPADSDILYLAAGRAGDAIAGGVFRSIDGGLNWTQGIGTEDL
                     RVESLVLDTSSPPGARVLYAGADTKGVLKSFDGGKSWTQVLKPSTVTTEAPNGFYKVM
                     VALAPVANPPNPAGPVV"
BASE COUNT         1482 a         3345 c         3109 g         1570 t
ORIGIN      
        1 gcaagggcac ttcagcgtat cgagcacctt acagttcgga gtggggtcag gtctccactt
       61 gccacatcgc ttcgcgatga tggctagggc acttcagcgt atcgagcacc ttaccgtgtc
      121 agtgaagacc tgaccccagg ctcgctctaa atcagctctg cctcgctgaa gaagaacgag
      181 acctctttcg cggcgttctc ggcggagtca ctgccgtgga tcgcgtttct ctcgatgtcg
      241 gtcccgtaga gatttcgcag cgttcccggc tccgccttct tcgaatcggt cgcgcccatc
      301 gtctggcgca ggtgggcaat ggcgttctcc cgctcgagcg ccgcgacgac gacaggccct
      361 tcggtcatga aacggaccag cccctcgaag aagggtcgtt cccggtgcac cgcgtagaag
      421 gcgcgcgcct gctcgggcgc gaggcgcagt cgcttcagtc cgcggacgcg gaatccctcc
      481 tcttccagtc gcgcgaggag agcgccggag cgcgccgccg aaacggaatc gggtttgaaa
      541 atcgtcagcg tccgctcgat cactcaaaac tccttcacga cgatcggcag gcgatcctgg
      601 gtggccaccc ccgcgtgcgc gagcgcttcg agaagctccc gtggccgctg cgagcccacg
      661 agcaaagtcg tgccgtcggc gcgggtcagg aggacgccca ggtttccgcg cgcgttcagg
      721 gcccgccgcc gccccgttcc ccgaattccc cacccgccgt actcgcgcac cggagcgtac
      781 tcgaccgctt cggcaagcgc gatttccgtc agcggtacgc gggtcttgta gagaaaaccg
      841 tatttcaccg cgagcgcatc ggaagtgacg gtcgtcgtca ggcgaaggga aaggagcgca
      901 ccgatgaggc cgatgacggc gacgaggagc gcgaccgccc acggcgagaa cgactgtgtc
      961 tcgtgataga gggtcgcccc attcatctcc cgagaacttc cttcatccgg gcgccgatgt
     1021 ctgccggcga ggggacgaca tggatcgagg ccgcctcgag cgctcgcacc ttctccgcgg
     1081 ctgtcccgcg cccccccgcg atgatcgctc cagcgtgtcc catccggcgt ccgggcggag
     1141 ccgtctggcc cgcgatgaag gcgacgacgg gtttcttgaa gtgacccgcg acgaagcacg
     1201 ccgcctcttc ctccgccatc ccgccgatct ctccgatgag gacgaccgcc tcagtccctg
     1261 cgtccgagcc gaacgcgccg aggcagtcca cgaagttcgt tccgttgacc ggatcgcctc
     1321 cgatgccgat caccgtggac tggccgaggc cgagtcgcgt cagctgatcg accgcctcgt
     1381 acgtgagggt tcccgatcgg gaaacgacgc cgatcgtgcc ggggcggtga atgcgcccgg
     1441 gcatgattcc gatcttgcac tcgcccggcg tgatgatgcc gggacagttc ggtccgatga
     1501 ggcgcacgcc ggcgcccgac gtatttttct catcgaggaa cttcttcacc cggagcatgt
     1561 cgttcgtcgg gatgccttcg gtgatgcaga cgatcacggc gatgcccgca tccgccgcct
     1621 cgacgatcgc atcggccgcg aacggcggcg ggacgaagat catcgaggca ttcgcgccgg
     1681 tcgcctccac ggcgcgcgcg accgtgtcga acacggggat ctcctcgtgg gtcgtgcccc
     1741 cttttcccgg cgtgactccc gcaacgaccc gggtgccgta ctcccggctc gcgagcgtgt
     1801 gaaaggttcc ttcgcgcccg gtcagaccct ggacgagcag gcgcgtttcg cggccgaccc
     1861 agatgctcac cggcttcttc cctcggtcac gatcgggatg cccgtgagct ccgagagcgt
     1921 ctcggagagc caggcgccga ggtcgcgcca ggacgggatc aggaggacgg ccgccgcccc
     1981 gacgacgagc gctgccgctc ccgtgcgcca gacgcgccgg gagtcccgct ccgcttcgtg
     2041 ccccagcgcg gcccggcccg cccgcgagag aaagagcggc agcacgaaga agaacacgag
     2101 gagcacgccc cagccgagga gaagcctcgg gttcgccgcg agcgtgggcc aacccttcca
     2161 cgtcgcgacg agcagcccca cgaagatcgg tccgagaaag aagccgaacg acttcagggc
     2221 gtagccgccg acgtgcttca cgcgcggcct ctcggaagag ccgccccgac cgccttgcgc
     2281 gccgcgtcgg cgagcccttc ggccggcgtg accgtgagcc cggactccga gaggatccgc
     2341 ttgccttcgt cgacgttggt gccctccaac cggacgacga ccggcagaga gagccccatc
     2401 tcgcggaccg cggcgaccac gcccctcgcg atgatgtcgc agcgcatgat gccgccgaag
     2461 atgttgatca gaatcgcgcg gacgttcgaa tcggacagga tgatccggaa ggcgtttttc
     2521 acctgctcct cggaggcgcc gcccccgacg tccagaaagt tcgccggctc cccgcccgcg
     2581 agctttacga ggtccatcgt cgccatcgcg agccccgcgc cgttgaccat gcagccgacg
     2641 ttgccgtcga gcttgatgta gttcaaaccg aacttcgacg cctcgacctc gagcggatct
     2701 tcctcggcca ggtcgcgcat ttcgcggatg tcggggtgtc ggaaaagcgc gttgtcatcg
     2761 aagttcatct tcgcgtcgag ggcgagaacg cggccggcct gggtcacgag gagcggattg
     2821 atctcggcga gcgaggcgtc ggtctcgagg tacgcgcgga agagcgcctg catgagagca
     2881 gtcgcctgtt tgaatgcatc tcccgccagg ccgagatcga aggcgagccg gcgcgcctga
     2941 aacgggaaca tgccgagcgc cggatcgacc ggctcgcgca ccaccgcttt cgggtcgcgc
     3001 gccgcgactt cctcgatctc catgccgccg gacttcgagg cgatcacgat cggccgcgcg
     3061 cgcgaccggt cgagggtaat gccgaggtac agctcgcggt ccgtctgcag cgcctcttcg
     3121 acgagcacct tgcggacgac gcgcccctcg gggccggtct gcggggtcac gagcgtcatc
     3181 ccgagaatcg ccttcgcatg ctggaacgcc tgctccgcgt cctgcgccac ccggatccct
     3241 ccgcccttcc cgcggccacc ggcgtggatc tgggccttga cgacgaccgg ccccccgatc
     3301 tgctgcgcga cttctcgcgc cgccgccggg ctgtcagcga cttctccccg cggcacggcg
     3361 acgccgtatt tcgcgaggat cgacttcgcc tggtactcgt ggaccttcac gttcgggatt
     3421 cctccttcag gtgaaggcgg cgcagccgaa agtgccgttc ttcgcgggtc accgaccccc
     3481 tcagaccgtc aggatgcgga acagctcgcg caccgaatcc gccgaccggc caagcgccga
     3541 cttctcgtcg gccgtgagct cgatctccat gatcttctcg acacccttgc gtccgagctt
     3601 gaccggaacg cccaggtaaa cgccggacat cccgtactcc ccctcgagat acgccgcgca
     3661 ggggaggatc ttcttctcgt ccttcaagac cgactcgacc atttccgcga ccgcggccga
     3721 cggcgcgtaa taggccgaac ccgtcttgta gtagttcacg atttcgattc cgccgtccct
     3781 cgtgcgcttg acgatcgcgt cgatccgctc cttcgagagg agctgcgtga tcgggatgcc
     3841 cgcgacggtc gagtaccgcg gcagcggcac catggtgtct ccatggccgc cgaggacgaa
     3901 agcgtgggtg ctctcgacgg aaacgtcgag ctcctccgcg aggaaccagc gcatcctggc
     3961 ggagtcgagg attcccgcca tcccgaagac gcgctcccgg ggaaacccgg agacgcgccg
     4021 cgccacctcg cacatggcat cgagggggtt cgagacgacg atgagaacgg ctttcggcga
     4081 gcgcctcgcc gcctctcgga ccacggcgcc gacgatctcc tggtttttct gcacgagatc
     4141 gtcgcgcgac attcccggtt ttcgcgcgag gcccgccgtc acgacgacga cgtcggagcc
     4201 gtcggacagc tcgtatgtat tcgagccttg cattcgggcg tcgaactttt cgaccggcgc
     4261 cgactccatc atgtcgagcg ccttcccctg cggaatacct tcgatgatgt cgatgaggac
     4321 gacgtcggcg atctccttct cgacgaggcg ttgcgccacc atggcgccga cgttcccggc
     4381 tcccacaacc gtcactttct gttgcatgag gacgcggatt gtatcggaag gccctcccgc
     4441 ggggagtgat gcgaaagaat ctcccaatac ccttacaatc gcggagcgtg gccgagcgtc
     4501 cggactacaa gagccatcct ctctggaacg aggcgatctc gctgacgcac gccgcgtacg
     4561 acctggcgga actcgtgcgg ccggccgatc cgatcacggc ccgccacctg cggcgcgcgg
     4621 cggtcgccat cccggcccac gtcgccggag cgctcacgtc cgacgaggag gggcggcgcg
     4681 agcacgtcct gaccgcgcgg ggtgcgctcg cggaaatctc ccggcaggcg gggcgcgcgg
     4741 aggcgtcggc cgatcccgcg accgccgccg agctcgcgcg gcgtgccgga gccctcgatc
     4801 gcgccgtctc gctcgagctc ggcctcgcgc agggagggat ttcctgatgc cgggcgagcg
     4861 ggcccggccg gctttgtacg ccgtgcttgc cggcgccctc gttctcgcga tggcggcatt
     4921 ctggttgccc cgctggtggg aaaaccggga gtaccggcag gcggaggcga tcgccggcgt
     4981 gggtgcgagg atcgtccccg gcaagatcgc cgaggcccgc aagcagctcg atccgggagt
     5041 gccgggttcc cgtgtgaccg ccgcgctcgg acccccctcc ttttccgtga agaccgaggg
     5101 ggcctcgtcg cacgagatct ggatctacta cttccaggat ggaacgctca cggtcaacct
     5161 gacggatggt tacgtcgcgc gcgcggcgac ggaattcggg cctccgaaga tccggaaatc
     5221 gcggcgtccc tgaaccggag aggacgcgaa tgagcgatcg cgaccgggcg gcggaccccc
     5281 gcagggagcc ctcgatccgg gtcgtcctca tgcccaagga cacgaacgcg cacggcacga
     5341 tcttcggcgg cgtgatcctc tcctacatcg accaggctgc ggcggtcgaa gcaaaacggc
     5401 acggcgccac tttcatcgtc acggtggcca tgcgggaggt cgtctttcac gagccggtct
     5461 acgtcgggga cctcgtctcg ttctacacgg ggctcgtgcg gatcggccga acgtccatca
     5521 ccgtttccgt cgaagtcttc gcgcagcgcg gcgaggggac cgccggcatg gtcaaggtca
     5581 ccgaggcgga agtgacgttc gtgaatctcg acgaggagag gaaaccgctg ccaatacctc
     5641 ggtagggggg taggtctcca ctttccactt tgcccgcaaa gtggcaagag ccagcatcgc
     5701 ggctcgtgaa gacctgacca aaaacggaag gtcccgattt ctcgggacct tcgtttcgcc
     5761 agccgagcga gagcccggca atgggtgttc cgcctgaacc tatattacca ctccgagtcg
     5821 tcttcctctt cggcggcagc ccccggttcc accggaagct cgacctcatc ttcttcgtca
     5881 gcgaggtcct cgagctccgg ttcgtcctcg acgtcctcct ctgccactgc gtcgagctcc
     5941 tccggccctt cgaccgccgt gatctccccg cctttccagg gggactcggg cgcgggccga
     6001 cgcggagccg ctgtcgtcgg cgatccggac gagcgcggcg tcgcggatcg cgacgggcgc
     6061 gacgccttgc gcgccggcgc gctcgacctc gacgttttct ttctcgcggt ccgcgccttc
     6121 ggacgcggct tcgccgcggt ccgccgcgtc cgcttcggaa cggccttgcg gcgggcggtt
     6181 cccttgcgcg ccacggaagt ctttcgcgcg gtccgcctcc cgcgggtcgt gctccgcttc
     6241 ttcgacgatg cgcgggagct cttgcgggtg ctgcgcaccc gcccggcgga cgaccgggag
     6301 gtcttccggg aacctccgcg ccccgctttt ttcgttttct tggccacgtg aaacctccct
     6361 gtattgcggc gggtacaggg tcgaggacct gttccccgcg gcaagttctg aaccggcttc
     6421 cgccggggtt cggatcatac aattccgagt ttgtcgaggc gagccgaagc gaatcgggtg
     6481 ctgatcacgc cgcggccggg cggcctgacg atcgagatcc ggccgctcgt gcgctcccgg
     6541 gcctggcggc ggcgtctcgt cgtcggagcg atcgtcatcg cggcggcggc cctcttcggg
     6601 atctcgcggc tcgtcccgct ctgggagacg ggactgcgcc gcgcgagctt cggtgacctt
     6661 cccctgccgc ttctcgtctt tctgacgctg gcggtcggga tatcgactcc gctgaccctg
     6721 atcggtctcg ccgccctggc cttcgcagag gagagcctcg aggtcacggc ggatgaggtc
     6781 gtcatctcga ccaccgcgtt cgaaaagacg cgggttcaga ggatttccct ccccgcgctc
     6841 gaccactggc gcgagacgcg ggcgcccctg ccgccctggt ggagctggag cgtcacacgc
     6901 ctcgccgctc gatcggcggg ccgccttcac ccgatcgccg gcggcgccgg tccccgcgag
     6961 aagcggcgga tcgggctcgc gctcgtgcag gcgacgaacc gtccgctgat cggcgacttc
     7021 ggccgcttga tcgccgcagc gcccgacccg cgctcgacaa acctagaatg atgccgatgt
     7081 ccgggacccg agcgacaccg cacggccacc tttcggagac gcctctggac tcctacgacc
     7141 ttccggcgga agttcgccgg ggaatcgccg acgcgggctt cgtccgggcg acccccatcc
     7201 aggagaaggc gcttccgatc gcgctcgccg gacgcgacgt cgccggccag gcccagaccg
     7261 gcaccggcaa gaccgccgcg tttctgatca cgatcttcgc gcggctgctc cgctcgcacc
     7321 cgaagaatcc cccgcgcggc ccgcggtctc tcgtcgtcgc gccgacgcgc gagctcgtcg
     7381 tccagatcga cgcggaagcg aaactcctcg gagtccacac cggtctctcc acgctggcgg
     7441 tctttggcgg gatcgactac cgcaagcagc gggaggccct gcggcaacgg cccgacctcc
     7501 tcgtcggcac gccgggccgg cttctcgact acgagcagca gggagcgacg acgtttcgcg
     7561 acgtcgaggt gctcgtcatc gacgaggccg accggatgtt cgacatgggc ttcatccgcg
     7621 acctccgccg gatcctgcgc cggtgcccgc cgtacgacgc ccgccagacg atgctcttct
     7681 ccgcgactct ttccccgcgg gtgatggagc tcgcgtacga gcacacgaac aacgcggaga
     7741 aagtcgagat cgagccggag cgggtgggtg ctcacggaat caccgaggtg ctctaccacg
     7801 tgtccacgcg cgagaagctc gcgctcctcc tcgggctcct cgcccgggaa ggcggcagcc
     7861 gggttctcat cttcgtgaac cgccgcacca ccgcggtcga gctcgtccgc gctctctccg
     7921 ccaacgggta ccccacgcgc gcgctcgccg gcaacgtgcc ccaggaaaag cgcctgaaga
     7981 tcctccgcga cttcaaggaa ggtcggctgg ccgtcctggt cgccacggac gtcgcgtcgc
     8041 ggggactcca catcgagggg gtctcgcacg tcgtcaacta cgacctgccg caggacgccg
     8101 aggattacgt ccaccggatc gggcgaaccg gacgggccgg cgccctggga acggcggtgt
     8161 cgatcgcgtg cgaggactac gtcttctcgc tcgatgccat ccagaagctc attggccgcg
     8221 agatcccggt cgagtgggca ccggcggcgc tcctggaggt cgggcggaac ttcgtggaaa
     8281 cgaaggggcc tcgtcggccg acacctgccc gaaggccccc gccgcgccgg cctccggcgg
     8341 aggtgaccta cggccgggag aagaggaaga gccggcggcg ctgagctgac gtcgacctcg
     8401 actttagtcg taggcggcga taggctgcac tcaactttcc gaggtgggtg ccatgcgttc
     8461 gccacggccg tcctccgtcg tcctcatcgc cgtcctcacc gtctcgacac tcttcgctgc
     8521 gggaggcggt cgcctccggc gcgagcgccc ggcggagagc cgatcccggg gcgttccccg
     8581 ggacctcgcc ttacccgccg cggatggagt ctcggacccc cgacgcctcg agcgggcgat
     8641 cgcgcagtgg aatcggctgc cgcgtgcctt ctccccttct tcccccgctc ttggatcgat
     8701 cggaggaacc gtctggaagc cgatcggacc gaacccgatc gtcgagcagg gctgctgcgg
     8761 ctccacgatc atcttcgccg caaatgggcg ggtgaactcg atcgcggtcg atccgacgaa
     8821 ctccgacgtg atctatctcg gatcggccgg gggcggcgtg tggaagagcg ttgaccaggg
     8881 ggcgcattgg aagcccgtca ccgaccagga ggtgtcgctc gggatcggca gctcccatgc
     8941 aatcgcgatc gatccgaacc atccgagcac cgtctacgcg gggacgagct catggtcgct
     9001 gctgaacccc ggcgacaggg cacagtcccg tggcatcctg aagtcgacgg acggcggcgc
     9061 gagctgggtc gtgctcggct ccggtacacc gtcgggcaac gtcgggaacg ccgaagactt
     9121 ctttcgcgac cggaacatca gcacgatcct cgtggatccc gccgacagcg acatcctcta
     9181 tctcgcggca ggacgggccg gcgacgcgat cgccggagga gtcttccggt cgatcgacgg
     9241 cggcctgaac tggacacagg gcatcggaac ggaggacctg cgggtcgagt ctctggtcct
     9301 ggatacgtcg tcgcctcccg gcgcccgggt gctctacgcg ggagccgaca cgaagggagt
     9361 cctgaagtcg ttcgacggcg gcaagagctg gacgcaggtg ctgaagccga gcaccgtcac
     9421 gacggaggca cccaacggct tctacaaggt gatggtcgcc ctcgcgccgg tcgcgaaccc
     9481 tccgaacccg gcagggccgg tggtct
//
LOCUS       DASWAV010000396         6128 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U2665
            k141_101658, whole genome shotgun sequence.
ACCESSION   DASWAV010000396 DASWAV010000000
VERSION     DASWAV010000396.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960316
            Sequence Read Archive: SRR10260007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 6128)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6128)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:47:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,929
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6128
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_101658"
                     /isolate="SMAG_U2665"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.36444 N 132.31889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..319)
                     /locus_tag="VF958_08295"
     CDS             complement(<1..319)
                     /locus_tag="VF958_08295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009606730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate/isopropylmalate family dehydrogenase"
                     /protein_id="HEX9149144.1"
                     /translation="MSSSKKRVVTLIRGDGIGPEVADAVVSILDAAGAPIEFEEAIVG
                     RRAEETDGDPLPNAVVESIRRNRVALKAPVATPIGKGFQSVNVRLRQTLELYANLRPV
                     KSVP"
     gene            complement(316..1596)
                     /locus_tag="VF958_08300"
     CDS             complement(316..1596)
                     /locus_tag="VF958_08300"
                     /inference="COORDINATES: protein motif:HMM:NF013741.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HEX9149145.1"
                     /translation="MNAKYLIFLVLLALSLGLSAFFAGAETALVSLSRIDLQRLRETS
                     DRRAGVLSDLKRNSSRLLATILIGQNLFITAASSLATAIATGLLGETYGVPAAILFST
                     LALFVFAEMTPKAIAAASPVSISRAVALPMAWTTRLLAPLVNVLVRISTGMLRLFGVP
                     EKTPTLTEAEMKSLFNLGAEEGVIHGEERKRLHKVLEFGDRTVRDIMVPRTKVVAVPE
                     TARFEDVRLVLKEHKFSRLPVYRGTLDNVVGILNAKDLFDLSDEEEGNFSVDRYLDPP
                     FLVPEFKRAEDLFREMRRRRTHMAIAVDEHGGTAGMATIEDAIEELLGPIQDEYDEEE
                     TPGFVAAGDRTYLLEGSFRLDDIEEQFGLSFPRDEAETIAGHLMLRFGRIPRKGERWK
                     GRRAEFIVEDATPTAIRSVRMILLPPPAPPPAAG"
     gene            complement(1593..2672)
                     /gene="queG"
                     /locus_tag="VF958_08305"
     CDS             complement(1593..2672)
                     /gene="queG"
                     /locus_tag="VF958_08305"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0052693 - epoxyqueuosine reductase
                     activity [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HEX9149146.1"
                     /translation="MSEPSDRVAAARIFREEAVRAGFSRVGIARAGEPPRFDRFIEWI
                     AAGRHAAMTYLQRTASLRAAPERLLEGARSVVCLAAPHPTKAPVAADGSRVARYTVGA
                     DYHGTLRARATRAARAAAARIGGEIRWRVCVDSTPIAERSFAAAAGLGWIGKNGCLID
                     SELGSYLLLAEIVTDADLPPDEPAAEMCGSCARCLESCPTDAFLAPGLLDAKRCLAYW
                     TIEHRGPIPDDMKIALGDHVFGCDICQEVCPWNEPARRGPSLPSPPGRGIGGEGVVSP
                     PIPTRAEWLAMGPGEWRRRFGATALNRAGRRGLHRNAAASAGATCDRSVLPALSRARR
                     TGDLGLADAARWAADRLESGVERTT"
     gene            2683..3828
                     /locus_tag="VF958_08310"
     CDS             2683..3828
                     /locus_tag="VF958_08310"
                     /inference="COORDINATES: protein motif:HMM:NF013740.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HEX9149147.1"
                     /translation="MVSETGDNLAAGGAALRNEERFYPRVFGLVVAGLLAAALYSILK
                     PFTGPILWALLLAFLLDPVNARLRRRLGGRRREGLSAFLMTVLVTLAIILPASLTALA
                     FAGQAADLIQRLSSVAQRYRIARPSDVVRLPVVDRLVHWLADRTPITVDQVQDWIVNG
                     LRTALEFALSNSKSVFLGALGAVIGLMLMLFVLYFFFRDGEEMISRGMALVPLDEPRK
                     RRLQSHIAEVTRAVFYGNVMTAAVQGALIGIAFWISPLPSPVVFGVLGAVFSLIPAVG
                     TGIVMVPAVIALAVQGQWGWTIFMFVWAVGVVGLADNFVRPLFISGRAQISTLPVFIG
                     AIGGVAAFGPIGLFLGPIVIALVLAFVRFAEEARAQAPAGSQPSVKT"
     gene            complement(3814..4791)
                     /locus_tag="VF958_08315"
     CDS             complement(3814..4791)
                     /locus_tag="VF958_08315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9149148.1"
                     /translation="MLPRLAEAVESLFGGRLAPVAAFARGAASVTLALAIALPLLRAA
                     LSFTAFRYGWETALVVRCPRCRRLVADPDVGICPAGHPIRFPPGAARREARRRRFHRM
                     ARAAASYRFVLPGVIALGAVAGFQACGVSRVEGSLATLTAATGYLFFAAALALAALAA
                     SPKPRGAPERALHAGTAAACLLPAIVLALLARAFEPPHPHAIGYVWSTPTSFYASTGG
                     RGQRLGDPRMEVEALLVEARAPAFGIVWQGLEGFRSHSRIIKWTGRGGFTARLLSRWA
                     SPLSRRGVFLSRSTQTVPLPPNVKIWIVSEPGRIRFTTEGDHDLGSGLD"
     gene            complement(4804..5976)
                     /locus_tag="VF958_08320"
     CDS             complement(4804..5976)
                     /locus_tag="VF958_08320"
                     /inference="COORDINATES: protein motif:HMM:NF012894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HEX9149149.1"
                     /translation="MRRSLTIVSIILTLFVAGCSSGPPPREIAEARLAVQDAKNASAD
                     QLATRDYDAALVNLRMAENTWDARKDAAGAAHWARMAEATARLAQYRAEARSADDALR
                     RETERKRLGEIAVRDAEIAALQARARTEAEKRAAEAEARALAERRASEERLAAQEAAA
                     REREQARADLEARLAAERAGAEREAAARSQAERDRAAAELEKTRAELEASRQAAEDAR
                     RAVEAERQKVEEQRKAQQAHADELARLEKEQQKTREELRATLSQLATVRQEARGLIVT
                     LPGSIYFDVNKSDVKPAMRARMTEIAKALATVPDQHVLVEGHTDSDGSSEYNLQLSRL
                     RANSVRSVLVAGGVSPDRIESQGYGETRPVATNANAAGKAQNRRVEIVIEGGAAAQ"
BASE COUNT          869 a         2136 c         2101 g         1022 t
ORIGIN      
        1 ccggaacgct cttgacgggt cgcaggttcg cgtaaagctc gagcgtctgt ctcagccgta
       61 cgttgacgga ctggaatccc ttaccaatcg gcgtggcgac gggcgccttg agggcgacgc
      121 gattgcgacg aatcgactcg acgaccgcgt tgggaagagg atcgccgtcc gtctcctcgg
      181 cacggcgtcc gacgatcgct tcctcgaact cgatgggcgc tcccgccgcg tcgaggatcg
      241 acacgacggc gtcggcgact tccgggccga tcccgtcgcc gcgaatcagc gtgacgaccc
      301 gcttcttcga cgagctcatc cggcggcggg aggcggagcg ggtgggggga gcaagatcat
      361 ccggacgctg cggatggctg tcggcgtcgc gtcctccacg atgaattctg cccgacgtcc
      421 cttccaacgc tctcccttcc tcggaatgcg cccgaagcgg agcatgagat ggccggcgat
      481 ggtctccgcc tcgtcacgcg gaaacgatag cccgaactgc tcctcgatgt cgtcgaggcg
      541 gaagctccct tccaggaggt aggtccgatc tccagcggcg acgaaccccg gagtctcctc
      601 ctcgtcgtac tcgtcctgga tcggcccgag cagctcctcg atcgcgtctt cgatcgtggc
      661 catccccgcc gttcctccgt gctcgtcgac ggcgatcgcc atgtgagtgc gccgccgtcg
      721 catctctcgg aacaggtcct cggcgcgctt gaattcggga acgagaaacg gcgggtcgag
      781 gtatcggtca accgaaaagt tgccctcttc ctcgtcggag agatcgaaga ggtccttcgc
      841 gttcagaatg cccacgacgt tgtcgagtgt gcctcggtag acgggaaggc gagagaactt
      901 gtgctccttc aggacgagcc tcacgtcctc gaagcgcgcg gtttcgggga ctgcgaccac
      961 tttcgtccgg ggcaccatga tgtcgcgcac ggtccggtcg ccgaactcca ggaccttgtg
     1021 gaggcgcttt ctctcttcgc cgtggatgac gccctcttcg gcgccgagat tgaagagact
     1081 cttcatctcc gcctcggtca gggtcggcgt cttttcggga acgccgaaga gccgcagcat
     1141 accggtcgag atccggacga gaacgttgac gaggggtgcg agcagccgcg tggtccacgc
     1201 catcggcaga gcgaccgcgc gggagatcga caccggggag gccgccgcga tcgccttggg
     1261 ggtcatctcc gcgaagacga acagcgcgag cgtcgagaaa agaatcgcgg cgggaacgcc
     1321 gtacgtctcg ccgagcaacc ccgtcgcaat cgctgtggcg agcgacgatg ctgcggtgat
     1381 gaacaggttc tgcccgatca ggatcgtcgc gaggagccgc gagctattcc tcttcagatc
     1441 cgagagcaca cccgcgcgtc ggtcgctcgt ctcccgcagc ctctgaagat cgatgcgcga
     1501 gagggagacg agcgcggttt ccgcgcccgc gaagaacgcg gacaggccga gcgagagtgc
     1561 gagcagcacg aggaagatca ggtatttcgc gttcatgtcg tcctttcgac gccgctttcg
     1621 agccgatccg cggcccagcg ggcggcgtcc gcgagcccga ggtcgccggt tctccgcgct
     1681 cgcgacaggg cggggagcac cgagcgatcg caagtcgcac cggccgaggc ggcggcgttt
     1741 ctgtggagac cgcgccggcc ggcccggttc agggcggttg ccccgaaccg tcgtctccat
     1801 tcgccgggcc ccatggcgag ccactccgcc cgcgtcggga tcggtggcga tacaaccccc
     1861 tcacccccga tccctctccc ggggggagag gggagcgatg ggcctctccg tgcgggttcg
     1921 ttccaggggc acacctcctg gcagatgtcg caaccgaaga cgtggtctcc gagcgcgatc
     1981 ttcatgtcgt cggggatcgg gccgcggtgt tcgatcgtcc agtacgccag gcagcgcttc
     2041 gcgtccagga gtccaggtgc caggaacgcg tcggtcggac agctctcgag gcagcgcgcg
     2101 caggagccgc acatctccgc ggccggttcg tcgggaggaa ggtcggcgtc ggtcacgatt
     2161 tcggcaagga gaagatacga gccgagctcc gaatcgatca ggcaaccgtt cttgccgatc
     2221 caccccaacc ccgcggctgc tgcgaaggag cgctccgcga tgggcgtcga gtcgacgcag
     2281 acgcgccagc ggatctcgcc gccgatacga gcggctgccg cccgggccgc gcgggtcgcc
     2341 cgcgcgcgca gggtcccgtg gtagtcggcg ccgacggtgt agcgcgcgac ccgggacccg
     2401 tccgcggcga caggggcttt cgttgggtga ggggcggcga ggcagacgac cgagcgcgcg
     2461 ccctcgagga ggcgctcggg cgcggcccgg agactggccg tgcgttggag gtacgtcatt
     2521 gccgcgtgac ggcccgcggc aatccactcg atgaagcggt cgaagcgcgg cggttcaccg
     2581 gcccgcgcga tgcccacccg ggagaaccct gcccgcacgg cctcctcgcg gaagatccgg
     2641 gctgctgcga cgcgatcgga cggctcgctc atcccgcggc gaatggtatc ggagaccggc
     2701 gataatctcg cggcgggagg ggcggcgctg cgaaacgagg agcggtttta tccgagggtt
     2761 ttcggcctcg tcgtggcggg gctgctcgcg gcggccctgt attcgatcct gaaacccttc
     2821 accgggccga tcctctgggc gcttctgctc gcgttcctgc tggacccggt gaacgcgcgg
     2881 ctgcgccgcc ggctgggcgg ccgccgccgc gagggtctgt cggcattcct catgacagtt
     2941 ctcgtcacgc tggcgatcat cctgcccgca tccctgacag cgctcgcttt cgccggacag
     3001 gcggccgacc tgatccagcg gctctcgagc gtcgcacagc gctaccggat cgcgcggccg
     3061 agcgacgtcg tccggctccc ggtcgtcgat cgcctcgtcc actggctcgc cgaccgcacc
     3121 cccatcaccg tcgaccaggt ccaggactgg atcgtcaacg gactccgtac agccctcgaa
     3181 ttcgcgctct ccaacagcaa gtctgtcttc ctgggcgccc tcggcgcggt catcggtctc
     3241 atgctgatgc tgttcgtcct ttacttcttc ttccgcgacg gagaggagat gatctcgcgc
     3301 ggcatggcgc tcgtgccact cgacgaaccc cgcaagcgcc ggctccaatc gcacatcgcc
     3361 gaggtcacgc gggcggtttt ctacggcaac gtgatgacgg ccgccgtgca gggcgctctg
     3421 atcggaatcg cgttctggat ctcgccgctc ccatccccgg tcgtcttcgg cgtgctcgga
     3481 gcggtgtttt ctctcatccc cgcggtgggg accggaatcg tgatggtgcc ggccgtcatc
     3541 gcactcgccg tccagggcca gtggggctgg acgatcttca tgttcgtctg ggccgtcggc
     3601 gtggtcgggc tcgccgacaa cttcgtccgg ccgctcttca tctccgggcg cgctcagatc
     3661 tcgactctgc cggtgttcat cggcgcgatc gggggcgtcg ccgcgttcgg ccccatcggg
     3721 ctctttctcg ggccgatcgt catcgccctc gttctcgcct tcgtccggtt cgccgaggaa
     3781 gcgcgggcgc aggcgccggc ggggagccag ccgtcagtca agacctgatc cgaggtcgtg
     3841 atcgccctcc gtcgtgaacc ggatcctgcc cggctcggaa acgatccaga tcttcacgtt
     3901 cggcgggagc ggcaccgtct gcgtggagcg tgagagaaag acgccgcgcc gcgacagcgg
     3961 gctcgcccag cgcgagagaa ggcgggccgt gaatccgccg cgcccggtcc acttgatgat
     4021 ccgcgagtgg cttcgaaagc cctccagccc ctgccagacg attccgaatg cgggagcgcg
     4081 cgcctcgacg agtagagctt cgacctccat ccgaggatct cccaaccgct gtccgcggcc
     4141 tcccgtcgac gcgtagaagg acgtcggtgt gctccacaca tagccgatgg cgtgggggtg
     4201 gggcggttca aatgcccggg cgaggagagc gagcacgatc gccgggagga ggcacgccgc
     4261 cgccgtaccc gcatgaagcg cgcgttccgg tgcgccgcgc ggtttcggcg aggccgccag
     4321 agccgccagg gcgagggcgg cggcgaagaa gagatagccg gtcgccgcgg tgagcgtcgc
     4381 gagcgaaccc tcgaccctcg atacgccgca tgcctggaat cccgcgacgg cgccgagggc
     4441 gatcacgccc ggaagcacga agcggtagga cgccgcagcc ctcgccattc ggtggaagcg
     4501 ccgccggcgg gcttcgcggc gcgcggcgcc cggaggaaag cggatcgggt gtcccgccgg
     4561 gcagatccca acgtccgggt ccgcgacgag ccgccggcat cggggacaac ggacaacgag
     4621 cgcggtctcc caaccgtatc ggaacgcggt gaacgacagc gccgcccgga gaagaggcag
     4681 agcaatcgcg agcgccaggg tgacgctcgc ggcgccccgg gcgaaggcgg cgaccggcgc
     4741 gagccgtcct ccgaagagac tttcgaccgc ttccgcgagc cgcggcagca tgcggacgac
     4801 gtcttactgc gcggccgcgc ccccttcgat gacgatctcg acccgccggt tctgcgcctt
     4861 gccggctgcg ttggcgttcg tcgcgaccgg ccgcgtctcg ccatatccct gcgattcgat
     4921 gcggtccggc gatacgcccc ccgcgacgag aaccgagcgc accgaattgg cgcgcagccg
     4981 cgacagctgg aggttgtatt ccgacgagcc gtcggagtcc gtgtgaccct cgacgaggac
     5041 gtgctgatcc ggaacagtcg cgagcgcctt cgcgatctcc gtcatccggg cgcgcatcgc
     5101 gggcttcacg tcggacttgt tcacgtcgaa gtagatgctg ccgggaagcg tgacgatcag
     5161 accacgggct tcctggcgca ccgtcgcgag ctgcgacagc gtggctcgca gttcctcccg
     5221 ggtcttctgc tgctccttct cgaggcgcgc gagctcgtcc gcgtgcgcct gctgtgcttt
     5281 ccgctgctcc tcgaccttct ggcgctccgc ctccacggcg cggcgcgcgt cctcggccgc
     5341 ctggcggctc gcctcgagct ccgcccgggt cttctcgagc tcggcggcgg cgcgatcacg
     5401 ctctgcctgc gaccgggcgg ccgcttcccg ttccgccccc gcccgctccg cagcgaggcg
     5461 agcctcgagg tcggccctcg cctgttcgcg ttcgcgcgcg gcggcctcct gcgctgcgag
     5521 gcgttcctcg gaggcgcggc gctcggcgag ggctcgcgct tccgcctcgg cggcacgctt
     5581 ctccgcctcg gtccgcgccc gggcctggag ggccgcgatt tccgcgtcgc gaacggcgat
     5641 ctcacccagg cgcttgcgtt cggtctcgcg gcggagagcg tcgtcggcac tgcgcgcttc
     5701 ggcgcggtat tgcgcgagcc gcgcggttgc ctccgccatc ctcgcccagt gcgccgcgcc
     5761 ggctgcgtcc ttcctcgcgt cccacgtgtt ctccgccatc cgcaggttga cgagtgcggc
     5821 gtcgtagtcg cgcgtcgcga gctggtcggc gctcgcgttc ttcgcgtcct ggaccgccag
     5881 ccgcgcctcc gcgatttcgc gcggcggggg cccggacgaa cagcccgcga cgaacagggt
     5941 gagtatgatc gaaacgatcg tcaggcttct gcgcatcgga ctacctcctt cacggagtcg
     6001 gcgcggaccg aatctgtcgc gtcctcgcct cgagctcgtc ggcttgctgt ttgaaagttg
     6061 cggcttcgct tcgcgtccgc tcggcgttcg cctgcgcctc cgcggcgcgg gcgtattcgc
     6121 gggcttcc
//
LOCUS       DASWAV010000397         2495 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U2665
            k141_462102, whole genome shotgun sequence.
ACCESSION   DASWAV010000397 DASWAV010000000
VERSION     DASWAV010000397.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960316
            Sequence Read Archive: SRR10260007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2495)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2495)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:47:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,929
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2495
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_462102"
                     /isolate="SMAG_U2665"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.36444 N 132.31889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            365..814
                     /locus_tag="VF958_08325"
     CDS             365..814
                     /locus_tag="VF958_08325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9149150.1"
                     /translation="MRNRTLTAVAALACASLVACSTVATLGTDPNTALNELTAVHKTR
                     DAAIAGKDAAALAKQHAPDLTATLADGTKLDRAGFEALLQEKAEVIAPSHITRLTQEE
                     SVYVAVVTPGLQGPGSALRETWKRTGAGWMLKKVEEVSAPSAIAQKN"
     gene            complement(858..2087)
                     /locus_tag="VF958_08330"
     CDS             complement(858..2087)
                     /locus_tag="VF958_08330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014561627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9149151.1"
                     /translation="MNDIAPEYVKLVLAVGTHDKDYVDAFYGPAEWKTNAEAAKKPLG
                     AIAAEADALIGRLQALPEPADEMLRLRLSYLTYQLRALAARVAILGGKKLTFDEESRA
                     LYDAVAPVNTEEHFRAIRDELDRALPGAGAAASLVDRYEAWRKDFVIPKEKVDSVFQT
                     AISECRARTTPHMTLPPGESFTVEYVTAKTWSGYNWYQGGYKSLIQVNTDFPITIDRA
                     IDLACHEGYPGHHVYNTLLEKNLVHDRGWVEYSVYPLFSPQSLIAEGSANFGIEMAFP
                     GRERLEYEKSKLFPLAGLDPTRAEEFDRVRRIVDRLAYAGNEAARRYLNGEIDAAAAA
                     KWLAEYALYPRDRAEQRVRFIDQYRSYVINYNLGKDLVHRYVESHGAQADNPNKRWEV
                     FEKLLSSPMLPSQLKSP"
     gene            2216..>2495
                     /locus_tag="VF958_08335"
     CDS             2216..>2495
                     /locus_tag="VF958_08335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HEX9149152.1"
                     /translation="MKAIRVDQPGGPEAMRLAEVPDPKPREGEVLVRNEAAGVNFIDV
                     YHRSGAYPLTFPLIPGQEGAGTVVALGAGVTEFAAGDRVAFASGSATYA"
BASE COUNT          404 a          829 c          842 g          420 t
ORIGIN      
        1 gcagcttcgg tccgctccct ctccgccctg gcgaggagag cggcacgttc ggtgcggcga
       61 cccgcgagcc acgcaacgac gccgatcccc gcgagcagca gggcgaggac gacggtctga
      121 cccgagacgg atccccgttc ccttcgagac atgggttttt cgggattcca tcacggctcg
      181 cggccgatga gacttttttt cgcgccgacg cgggtcgttc gagcgcactt cgctgcccga
      241 gttgcccgcg aatgtctcat tgatgacggc gggccacgcg gccggatcgc ctttctcgag
      301 tccgacaaaa cctattccgg cggcgtccgt ctcaactgct gaaggaaaac aaaggaggtc
      361 tcgaatgcga aatcgaactc tcaccgcggt cgccgcgctc gcgtgcgcca gcctcgtcgc
      421 gtgctcgacc gtcgcgacgc tcggcacgga cccgaacacg gcgctgaacg agctcaccgc
      481 cgtccacaag acgcgagacg cggcgatcgc cggcaaggac gccgcggcgc tcgcgaagca
      541 gcacgctccc gacctcacgg cgacgctcgc cgacggaacg aagctcgatc gcgccggctt
      601 cgaagcgcta ctccaggaga aagcggaagt cattgcgccc tcgcacatca ccaggctcac
      661 gcaggaagag agcgtctacg tcgcggtcgt gactccgggt ctccagggac cggggtcggc
      721 ccttcgcgag acgtggaagc gaaccggcgc gggttggatg ttgaagaagg tcgaggaagt
      781 ttcggcgccg tcggcaatcg cgcagaagaa ctaggcccct cgcccggacc ctctccccgc
      841 gccgcgggga gaggggatca tggcgacttc agctgtgagg ggagcatcgg ggacgacagc
      901 agtttctcga acacttccca gcgcttgttc gggttgtcgg cctgagcgcc gtgcgactcg
      961 acgtaccggt gcacgaggtc cttgcccagg ttgtagttga tcacgtagct cctgtattgg
     1021 tcgatgaagc gaacgcgctg ctcggcgcgg tcgcgcggat agagcgcgta ctcagccagc
     1081 cacttcgccg cggctgcggc gtcgatctcg ccgtttaggt atctccgcgc cgcctcgtta
     1141 ccggcgtagg cgaggcgatc aacgatccgc ctcacgcggt cgaattcctc cgctcgcgtc
     1201 gggtcgagtc ccgccagcgg gaaaagcttc gacttctcgt actcgaggcg ctcgcggccc
     1261 gggaacgcca tctcgatccc gaagttcgcg ctgccctccg cgatcagcga ctgcggagag
     1321 aacagcggat agacggagta ttcgacccac ccccggtcgt gaacgaggtt cttctcgagg
     1381 agcgtgttgt agacgtggtg gccggggtat ccctcgtggc aggcgaggtc gatcgcgcgg
     1441 tcgatcgtga tcggaaagtc ggtgttgacc tggatcaggc tcttgtagcc gccctggtac
     1501 cagttgtagc cgctccacgt ctttgccgtg acgtactcga ccgtgaagct ctcgcccggt
     1561 ggaagggtca tgtgcggcgt ggtgcgggcc cggcactcgg agatcgcggt ctggaagacc
     1621 gagtccacct tttccttcgg gatcacgaag tccttgcgcc aggcctcgta gcggtcgacg
     1681 agcgacgccg cagcgccggc gccgggcaac gcgcggtcga gctcgtcccg gatcgcacgg
     1741 aagtgctcct cggtgttcac gggcgcgacc gcgtcgtaga gggcgcgcga ctcctcgtcg
     1801 aaggtgagct tctttcctcc gagaatcgcg acgcgggccg cgagcgcccg gagctgatac
     1861 gtcaggtagc tcaagcgcag ccggagcatc tcgtccgccg gctccgggag cgcctgcagc
     1921 cgtccgatga gcgcgtccgc ctccgccgcg atcgcgccga gcggcttctt cgccgcctcg
     1981 gcgttcgtct tccattcggc cgggccatag aacgcgtcca cgtagtcttt gtcgtgagtc
     2041 ccgaccgcga ggacgagctt cacgtactcc ggggcgatgt cgttcatccc gctgagagta
     2101 ttcgacgcgg ccgccgccgg tgcgtgcgcg caggccggag cgaggaggga tcccaggatc
     2161 gcgccggcaa taacacagcg catcgtttgc atgggcgaag gataatgcgg ccgcgatgaa
     2221 ggcgatccgg gtcgatcagc cgggcggacc cgaggcgatg cgcctcgccg aagttccgga
     2281 cccgaagccc cgcgagggag aagttctcgt gcggaacgag gcggcgggag tcaacttcat
     2341 cgacgtctac caccgcagcg gcgcctatcc gttgacgttc ccgctgatcc ccggacagga
     2401 gggcgcgggc acggtcgtcg cgctcggcgc gggcgtcacc gagttcgccg ccggcgaccg
     2461 cgtcgcgttc gcctcgggat ccgccactta tgcgg
//
LOCUS       DASWAV010000398         3071 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U2665
            k141_101727, whole genome shotgun sequence.
ACCESSION   DASWAV010000398 DASWAV010000000
VERSION     DASWAV010000398.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960316
            Sequence Read Archive: SRR10260007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 3071)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3071)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:47:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,929
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3071
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_101727"
                     /isolate="SMAG_U2665"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.36444 N 132.31889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(227..2359)
                     /gene="glgP"
                     /locus_tag="VF958_08340"
     CDS             complement(227..2359)
                     /gene="glgP"
                     /locus_tag="VF958_08340"
                     /inference="COORDINATES: protein motif:HMM:TIGR02094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucan family phosphorylase"
                     /protein_id="HEX9149153.1"
                     /translation="MEAFGNELRAPALPSNLTPLLALSRDLRWAWRPSIRALFSSLDP
                     ALWTELRGNPAAFLRRLFRERLDAAAADSVFLSTLYAIASELALEDTTHSIHPGVRSL
                     WARGDRVAYFSAEFGVTESLPIYAGGLGVLAGDVLKSASDLGLPMMGVGLFYREGYFR
                     QLLDREGWQGDENLPLDPDELPLSLPPVQDGGAPVVTLELGDRAVQLLIRVAKVGRVP
                     LFLLDADLPENHPDDRALTARLYSGDQEMRIRQEITLGIGGLRALKQLGLAASIRHIN
                     EGHAAFAVLEKIRQLVAEEGRTVAEAREVAAAGNVFTTHTPVPAGIDRFPAPLVEKYL
                     SGVARDCGISIEELMRLGREVPERKGEPFSMAVLALRHSAFANAVSQLHARVSRRLWR
                     DLLPELADVDVRIRSITNGVHRATWTDPEIAMLRISPNSRPEARLELWRTHERLRARL
                     VTFCRGRLAAWKREIGASEDEIGEAGRVLDPQALTIGFARRFATYKRATLLFSDPERL
                     KRLLDSRPIQVVFAGKAHPQDDPGKELLRDIVRFAESAEFRGRIVFVPDYDMGIARAL
                     VAGCDVWLNNPVRPHEASGTSGMKAAMNGGLNLSVLDGWWDEAPYEEAGFVIGEATDE
                     SVGEQAANALYDALEERVVPLFFERDEDGIPRGWIDKMVFSATRIARMFSSDRMVTEY
                     LELCYLPAAERRLAAMNGRVKQFAPEEA"
     gene            complement(2392..>3071)
                     /locus_tag="VF958_08345"
     CDS             complement(2392..>3071)
                     /locus_tag="VF958_08345"
                     /inference="COORDINATES: protein motif:HMM:NF023478.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF3536 domain-containing protein"
                     /protein_id="HEX9149154.1"
                     /translation="GETLERVENSGYARLTNYGEFLERFPPRLEAQVVPNSSWSCAHG
                     VGRWSDDCGCSADAAVSHPWRRPLREALDWLRDTLAPLYSAAASTLFTDPWAARNDSI
                     LLILDPSPGNVDRFFERNSVNALSEDERTTALGLLEIQRNALLMYTSCGWFFDDPAGL
                     ETRQVLRYAARAVELAEEHLGGSIEPLFLRLLERVRSGSGDRVDASEIYRTLRSPFTP
                     SAAVLPS"
BASE COUNT          537 a         1039 c         1034 g          461 t
ORIGIN      
        1 tcgaggaatc gctcggccat cggtccgacg tcgccgatgc cgaaccgccc gggaagcgag
       61 gtcgggtgga ggaggatccc ggccgctcga tcagtcatgg ggtcaggtct ccacttgcca
      121 catccggggt caggtctcca cttgccactt tgcttcgcaa agatggcaag ggcgtctcgg
      181 cagattcagc gccacggcgg ctcgtgaaga cctgaccccg atccggtcag gcctcttccg
      241 gcgcgaattg cttcactcgg ccgttcatcg ccgcgagccg ccgctcggcc gccggcaagt
      301 agcagagctc gaggtactcg gtcaccatcc gatccgacga gaacatccgc gcgatgcgcg
      361 ttgcggagaa gaccatcttg tcgatccagc cccgcgggat tccgtcctcg tcgcgttcga
      421 agaagagagg cacgacccgc tcctcgagcg cgtcgtagag cgcgttcgcc gcctgttctc
      481 cgaccgattc gtcggtcgcc tcgccgatga cgaagcccgc ctcttcgtaa ggcgcctcgt
      541 cccaccaccc gtcgaggacc gacaggttca agcccccgtt catcgccgct ttcatgcccg
      601 acgttcccga ggcttcgtga ggacggaccg ggttgttcag ccagacgtcg cagcccgcga
      661 cgagggcccg cgcgatcccc atgtcgtaat cggggacgaa gacgatccgc ccgcggaact
      721 ccgcgctctc ggcaaaacgg acgatgtcgc gcagcagctc ctttccagga tcgtcctggg
      781 ggtgcgcctt tccggcgaac acgacctgga tcggccgcga gtcgagcagt cgcttcagcc
      841 gctccgggtc gctgaacagg agggtcgccc gtttgtacgt cgcgaaccgc cgcgcgaagc
      901 cgatcgtcaa cgcttgcgga tcgagaacgc gaccggcctc cccgatctca tcctcggagg
      961 ccccgatttc gcgcttccag gccgccagac gcccgcggca gaacgtaacg agccgggcgc
     1021 ggagacgctc gtgcgtccgc cacagctcga ggcgcgcctc cggacgggaa ttcggggaaa
     1081 tgcgcagcat cgcgatctcc ggatccgtcc aggtcgcccg gtgaaccccg ttcgtgatcg
     1141 accggatgcg cacgtccacg tcggcgagct ccggaagcag atcgcgccag aggcggcgcg
     1201 agaccctcgc gtgcagctgg gagaccgcgt tcgcgaacgc cgaatgccgc agcgcgagga
     1261 cagccatcga gaagggctct cccttccgct ccggcacctc acgcccgagc cgcatgagct
     1321 cctcgatcga gatcccgcag tcgcgcgcaa ccccggatag gtatttctcc accagcggcg
     1381 ccggaaatcg gtcgatgccc gcaggaaccg gtgtgtgcgt ggtgaagacg tttccggccg
     1441 cggcgacttc ccgcgcctcg gcgacggtcc ggccttcttc agcgaccagc tgtcggatct
     1501 tctcgagcac cgcgaacgcc gcatgacctt cgttgatgtg ccggatcgaa gctgccaggc
     1561 cgagctgctt caacgcgcgc agccctccga tgccgagcgt gatctcctgc cggatccgca
     1621 tctcctggtc gccggagtag aggcgggcgg tcaaggcacg atcgtcggga tggttttcgg
     1681 gaagatcggc gtcgaggaga aaaagcggaa cccgaccgac cttcgcgacg cggatcagga
     1741 gttgaacggc gcggtcgccg agctccaggg tcacgacggg agctccgccg tcctgcaccg
     1801 gcggcagcga gagcggcagt tcgtccggat cgagtggcag gttctcgtcg ccctgccacc
     1861 cttcccggtc gaggagctga cggaaataac cctcgcggta gaacagcccc acgcccatca
     1921 tcggcaggcc caggtcgctc gcggacttca ggacgtcgcc ggcgaggact cccaggcccc
     1981 ccgcgtagat cggaagggac tcggtcacgc cgaactcggc cgaaaaatag gcgacccggt
     2041 cgcctcgggc ccacaggctc cggactccgg ggtgaatcga gtgggtcgtg tcctccaggg
     2101 cgagctccga agcgatcgcg tagagcgtgg agagaaacac ggagtctgca gcggcagcgt
     2161 cgagtctttc gcggaagaga cggcggagga acgccgcggg gttgccgcga agctccgtcc
     2221 acagcgccgg gtccagcgag gagaagagcg cgcggatcga cggacgccag gcccagcgca
     2281 ggtcgcggga gagggcgagg aggggcgtca ggttcgacgg aagagcgggt gccctgagct
     2341 cgttcccgaa agcttccatc gactgtcgga ggatagcaga ctgattcgcc gtcagctcgg
     2401 caggaccgcg gcggagggag tgaagggcga ccgaagcgtc cggtagatct ccgacgcgtc
     2461 gacccggtca cccgacccgc tccgcacccg ctcgagaagg cgcaggaaca gcggctcgat
     2521 cgacccgccg agatgctctt ccgcgagctc cacggcgcgc gccgcgtagc gcagcacctg
     2581 gcgcgtctcg agccccgccg gatcgtcgaa gaaccacccg cagctcgtgt acatgaggag
     2641 cgcgttgcgc tggatctcga gaagtccgag ggccgtcgtc ctctcgtcct cggagagcgc
     2701 attgaccgaa ttgcgttcga agaaacggtc gacgttcccg ggagaggggt cgaggatcag
     2761 aagaatcgaa tcgttgcgcg ccgcccacgg gtccgtgaag agtgtggacg cggcagccga
     2821 gtagagggga gcgagcgtgt cacgcagcca gtcgagggcc tcgcgcagtg gccgccgcca
     2881 cggatggctg accgcagcgt cggccgagca accacagtcg tcgctccagc ggccgactcc
     2941 gtgcgcgcag ctccacgagg agttcgggac gacctgcgcc tcgaggcgcg gtgggaaccg
     3001 ctcgaggaac tcaccgtagt tcgtcaggcg cgcgtaaccg gaattctcga cgcgttcgag
     3061 cgtttctccg a
//
LOCUS       DASWAV010000399         2890 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U2665
            k141_379329, whole genome shotgun sequence.
ACCESSION   DASWAV010000399 DASWAV010000000
VERSION     DASWAV010000399.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960316
            Sequence Read Archive: SRR10260007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2890)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2890)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:47:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,929
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2890
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_379329"
                     /isolate="SMAG_U2665"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.36444 N 132.31889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(147..356)
                     /locus_tag="VF958_08350"
                     /pseudo
     CDS             complement(147..356)
                     /locus_tag="VF958_08350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013587355.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase B"
     gene            463..1182
                     /locus_tag="VF958_08355"
     CDS             463..1182
                     /locus_tag="VF958_08355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013013400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX9149155.1"
                     /translation="MADERVALVTGGTRGIGLAVARRLALEGLAVAISGRNDGSIRDG
                     LERLRKEVPSGRFAGRAADARSEADQRSLVEWTAETFGRLDVLVNNAGVGEFDSVEKL
                     APERFRSVVETNLLGPYYATHFAVPLMKKSGGGFVVNIGSLAAVNAFAGGSAYNASKF
                     GLLGFSEAAMLDLRHQGIRVATILPGSVATEFGHSHGDRDHAWMLSAEDVAEAVADLI
                     RFPDRAIASRIDLRPSRPPKR"
     gene            complement(1193..1648)
                     /locus_tag="VF958_08360"
     CDS             complement(1193..1648)
                     /locus_tag="VF958_08360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012924704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low affinity iron permease family protein"
                     /protein_id="HEX9149156.1"
                     /translation="MSAGNRLERFSQAVSQWTGSSPAFALALGFVLVWAITGPLFGFS
                     QGWQMVINTGTTIATFLMVFLIQRAQNKESRAIQLKLNEIVAAIEGASNRLVGAEELS
                     EAELHRLHDRYVKLAEQVQTRHGSLKRAASVEETTGTHAATDAPARASR"
     gene            complement(1711..>2890)
                     /locus_tag="VF958_08365"
     CDS             complement(1711..>2890)
                     /locus_tag="VF958_08365"
                     /inference="COORDINATES: protein motif:HMM:NF014871.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0009166 - nucleotide catabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="5'-nucleotidase C-terminal domain-containing
                     protein"
                     /protein_id="HEX9149157.1"
                     /translation="RLSNVHDVVVKEVGGTAVGIFGLTTDAQPRDYVAYDYDPPARRA
                     AVERALTKLRTGGARAVIALTHQDLDEDVRLATEFPAIGLVVGGHEHFFLERNVGRTE
                     ITKADADAKTAVIHDLQISPDGVLDDSIRKVSLDERVEKDADVEKEVRAWLAELAVAV
                     KAQTGRDLQEVVGETENLLEGEEPAVRGRETALGNFLTDVIRDRMKTDFGFINGGAIR
                     INDNIPPGPIRNYDLEGIFYFDNQAVSFEITGAELLELLRISVAKVHTGHGRFLQVSG
                     IRFRYHVGGTPEQPVYTVEASDVEVSPRGSSGFRPLDLAARYSAASLDYLWENGYRDG
                     YPLFSKGRGGASPRRLDTGALPWRALTEEAIRSLPGRRVTSKIEGRIVRVENPASPPS
                     "
BASE COUNT          463 a          969 c          959 g          499 t
ORIGIN      
        1 aacggcgacc cccgatcgac gaggcccgcc gagagcgagt tggaccgcag gaacatcgag
       61 ctcgctgtgt acggctccag gaactccgcg agcgcggccc cgtccccccc ggagagcgtc
      121 ttcgcgtcca ccttcccggc acccggttac aactgtgacc ggagcaatcc caccaggcgt
      181 tccaggagct tctcggagag cgggcggtgc ttccactccg cgagcgtgac gcggtgcgat
      241 cgggccctgt cccgctcgaa cactgcagtc tgctcccgcg cgaagtctcc gtcgaacacg
      301 ttcagattcg cctcgtcgtt cagtcagaag gagcgattgt cgaagttggt cgaaccagga
      361 cgacggccat cgcgaccctc cccgacttct tcatcgcaca gtaccagacg ggtcgacgcg
      421 accggcgggc ggagcggcgg caatgtcgga tacgattccg ccatggccga cgagcgggtc
      481 gcgctagtga ccggaggaac gcggggaatc gggctcgccg tggcgcgccg cctcgccctc
      541 gaggggctcg cggtcgcaat ctccggccgg aacgatggga gcattcgcga cggtctcgaa
      601 aggctccgca aggaggtccc gtcgggccgt ttcgcgggtc gagccgccga cgcgcgaagc
      661 gaagccgatc agcgctcgct cgtcgaatgg acggccgaga cgttcggtcg cctcgacgtc
      721 ctcgtcaaca acgccggggt cggtgagttc gattcggtcg agaagctcgc tcccgaacgc
      781 ttccgatcgg tggtcgagac gaacctcctc gggccgtact acgcgacgca cttcgccgtc
      841 cccctgatga agaagtcggg gggagggttc gtcgtcaaca tcgggtctct cgcggcggtc
      901 aacgctttcg ccggcggctc ggcctacaac gcgtcgaagt tcggtctcct cgggttctcc
      961 gaggcggcga tgctcgacct gcggcaccag gggatccgtg tcgcgacgat cctccccggc
     1021 tcggtggcga ccgagttcgg acattcccac ggagatcggg atcacgcgtg gatgctctcg
     1081 gccgaggacg tcgcggaagc ggtggcggat ctcatccgtt ttccggaccg cgcgattgcc
     1141 tcgcggatcg acctgcgtcc ctcacgcccg ccgaaacggt aaacggcggc cctcagcggg
     1201 aggcccgcgc cggcgcgtcg gtcgccgcgt gcgtcccggt cgtctcttcg acggaagcgg
     1261 cgcgtttcaa cgacccgtgt cgggtctgga cctgctccgc gagcttcacg taacgatcgt
     1321 gcaggcgatg gagttccgct tcggaaagct cctccgcccc gacgagacgg ttcgaagctc
     1381 cctcgatcgc tgcgacgatc tcgttcaact tgagctggat cgcgcgcgac tccttgttct
     1441 gcgctcgctg gatcaggaac accatgagga aggtggcgat cgtcgtgccg gtgttgatga
     1501 ccatctgcca accctgcgaa aagccgaaga gcgggccggt gatcgcccag acgagaacga
     1561 aaccgagcgc cagcgcgaac gcgggagagc tcccggtcca ctgcgacacg gcctgagaga
     1621 aacgctcgag gcggtttccc gccgacaccg aaggtttcga ctccatggcg tgggcgggat
     1681 tgcaagggcc ctgccgctcc cgaacgagac tcaggacggg ggacttgcgg ggttttcgac
     1741 gcggacgatc cgcccttcga tcttcgatgt cacgcgccgc ccgggaagcg agcggatcgc
     1801 ctcttcggtc agcgcccgcc aggggagcgc gcccgtgtcg agccttcggg ggctcgcccc
     1861 gccgcgccct tttgagaaga gcgggtaccc gtcccggtat ccgttctccc agaggtagtc
     1921 gagggaggca gccgagtacc gcgccgcgag gtcgagtggc cgaaatcccg acgagccgcg
     1981 cggcgagact tcgacgtcgg acgcctccac cgtgtaaacc ggctgctccg gtgtcccccc
     2041 cacgtgatac cggaatcgga ttcccgagac ctgcaggaac cgtccgtgcc cggtgtgcac
     2101 cttcgcgacg gagatccgga gcagctcgag cagctccgct ccggtgatct cgaaggaaac
     2161 ggcctggttg tcgaagtaga agattccctc gaggtcgtag ttgcggatcg gcccgggagg
     2221 gatgttgtcg ttgatgcgga tcgcgccgcc gttgatgaat ccgaaatcgg ttttcatgcg
     2281 gtcgcggatg acgtcggtca ggaaattgcc gagcgctgtc tcccggcctc gaaccgcggg
     2341 ctcttctccc tcgaggaggt tctcggtctc gccgacgact tcctgaaggt cccggcccgt
     2401 ctgtgccttg accgcgacgg cgagctcggc gagccatgcc cggacctcct tttcgacgtc
     2461 cgcatccttt tcgacacgct cgtcgagcga gacctttcgg atcgaatcgt cgagaacgcc
     2521 gtcgggtgaa atctgaaggt cgtgaatgac ggccgtcttc gcatcggcgt cggccttggt
     2581 gatctcggtc cgccccacgt tgcgctcgag aaagaagtgc tcatgtcccc caacgacgag
     2641 cccgatcgcc ggaaactccg tcgcgagccg gacgtcctcg tcgaggtcct ggtgggtgag
     2701 cgcgatgacc gcgcgcgcgc cgccggtccg cagtttcgtg agcgctcttt cgaccgcggc
     2761 ccgccgcgcg ggcgggtcgt agtcatacgc gacgtagtcc cggggttgag catccgtggt
     2821 cagcccgaag atcccgaccg cggttcctcc gacctccttc acgacgacgt cgtgcacgtt
     2881 cgagaggcgc
//
LOCUS       DASWAV010000400         1583 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U2665
            k141_261551, whole genome shotgun sequence.
ACCESSION   DASWAV010000400 DASWAV010000000
VERSION     DASWAV010000400.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960316
            Sequence Read Archive: SRR10260007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 1583)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1583)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:47:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,929
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1583
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_261551"
                     /isolate="SMAG_U2665"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.36444 N 132.31889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1061
                     /locus_tag="VF958_08370"
     CDS             <1..1061
                     /locus_tag="VF958_08370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011523080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEX9149158.1"
                     /translation="LPLFAASLPVTTEGDFVVRDFRFASGETLRELTIHYRTLGAARR
                     DSRGRVENAVLVLHGTTGSGAQFVTENFAGVLFSDGELLDSRRFFLVLPDGIGHGRSS
                     KPSDGLRARFPHYGYDDMVTAQHRLLTEGLGVDHLRLVIGTSMGGMHAWVWGEKYPDF
                     MDGLVPLACVPTQIAGRNRMLRKMVSDDIRNDPDWRGGNYERQPRGLTAAMQILLIMV
                     SSPLQWHRTAPTREAADQFLEVEMRRRLASADANDFLYAFEASRDYDPSPLLERIRAP
                     VLAINSADDFVNPPELGLMERLMSRVPKGRYVLIPTGPETRGHGTHSYPALWQEHLRS
                     FIETLDRAGADSGSRPGR"
     gene            1210..>1583
                     /locus_tag="VF958_08375"
     CDS             1210..>1583
                     /locus_tag="VF958_08375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9149159.1"
                     /translation="MARSGSPKFFAAVLGVSLFVTAGPALAAKPAGRPPAPSLTCAGA
                     TGLSIAIQVCAGPGGAPGGFAIQWTTAESLAAGPDGLPGTADDSTWPASDDADLCTAS
                     FVGRAWFTKPELGGGECATVEVG"
BASE COUNT          249 a          563 c          519 g          252 t
ORIGIN      
        1 cgctccccct cttcgccgcc tcgctgcccg taaccaccga gggagatttc gtcgtcagag
       61 acttccgatt cgcaagcgga gagacgcttc gggagctcac gatccactac cgcaccctcg
      121 gcgctgcccg gcgcgattcc cgcggccgcg tcgagaatgc ggttctcgtg ctccatggaa
      181 cgaccggttc cggagcgcag ttcgtcaccg agaacttcgc gggggtgcta ttcagcgacg
      241 gagagcttct cgactcgcgg cgcttcttcc tcgtgctccc cgacgggatc ggccacggca
      301 ggtcttccaa gccctccgac ggcctccgcg cccgcttccc gcactacggc tacgacgaca
      361 tggtgacggc gcagcaccgc ctcctgaccg aagggctggg cgtcgatcac cttcgcctcg
      421 tcatcgggac ctcgatgggc ggaatgcacg cgtgggtctg gggcgagaag tacccggact
      481 tcatggacgg tctcgtgccg ctcgcctgtg tcccgacgca gatcgccggc cgcaaccgga
      541 tgctgagaaa gatggtctcc gacgacatcc gcaatgatcc ggactggcgc ggaggcaact
      601 acgagcggca gccgcgcggg ttgacggccg cgatgcagat tcttctcatc atggtctcga
      661 gcccgctcca gtggcacagg accgcgccga cccgcgaggc cgcggaccag ttcctcgagg
      721 tagagatgcg gcggcgactt gcatcggccg acgcgaacga cttcctgtat gcgttcgaag
      781 cttcccgcga ctacgacccc tcgcctctgc tcgagagaat ccgcgctcct gtcctcgcga
      841 tcaactcggc cgacgacttc gtgaacccgc cggagctcgg cctgatggag cggctgatgt
      901 cccgggtccc gaagggacgc tacgtgctca tcccgacggg ccccgagacc cggggccacg
      961 gaacccactc gtacccggcc ctgtggcagg agcacctgcg ttcgttcatc gaaacgctcg
     1021 accgcgccgg tgcggattct ggatcgcgcc cgggccggta atccgaccaa agttggacga
     1081 ccatcgtcca attgcgaaca ggccaccagc gggcgatcat gttttcggaa ctcccgacct
     1141 gaaaagcgat ggaaacccga cccgggcggc ggctgccggc agccgcgcgg ggagcggacg
     1201 gaggaggcta tggccaggtc ggggagtccg aagttcttcg ccgcggtgct cggcgtttcg
     1261 ctgttcgtca ccgccggccc ggcgctcgcc gcgaagcccg cggggagacc gccggcgcct
     1321 tccctcacgt gcgccggcgc gaccggtctg tcgatcgcca tccaggtctg cgccggaccc
     1381 ggaggcgcgc cgggcggttt cgcgatccag tggacgacag ccgaatcgct cgcagcgggt
     1441 cccgacggcc tacccggaac cgccgatgac tcaacgtggc cggcgtccga tgacgcggac
     1501 ctctgcacgg cgtccttcgt cggccgggcg tggttcacga agcccgagct cggcggcggc
     1561 gaatgcgcca ccgtcgaagt cgg
//
LOCUS       DASWAV010000401         1883 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U2665
            k141_780685, whole genome shotgun sequence.
ACCESSION   DASWAV010000401 DASWAV010000000
VERSION     DASWAV010000401.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960316
            Sequence Read Archive: SRR10260007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 1883)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1883)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:47:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,929
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1883
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_780685"
                     /isolate="SMAG_U2665"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.36444 N 132.31889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(29..1018)
                     /locus_tag="VF958_08380"
     CDS             complement(29..1018)
                     /locus_tag="VF958_08380"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024258.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair exonuclease"
                     /protein_id="HEX9149160.1"
                     /translation="MSQGRDPRIVDYGVLIARSQRSVPLFAPQEQVPPVTLSLVSSRR
                     IRVLLIADTHLGFDLPERPRSDRRRRGEDFFSAFERALQPAFRGEVDFVVHGGDVFFR
                     SRVKPGLVMRAFEPLKRIADSGVPVLVVPGNHERSAIPYPILAAHPRIHLFDRPRTIS
                     LNIRGVDVAVAGFPCERHEVRDTFARLVERTGWRSVPSDLRLLCFHQTVEGATVGPVG
                     YVFRTNADAIPGRAIPHGFAAVLAGHIHRHQLLTADLSGRSIGAPVFYPGSTEPTSAA
                     ERGEVKGYVTLELEPDADGKGCIRAWAFHALRTESLRRTRSPLCLSASSASLW"
     gene            1235..1831
                     /locus_tag="VF958_08385"
     CDS             1235..1831
                     /locus_tag="VF958_08385"
                     /inference="COORDINATES: protein motif:HMM:NF019265.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEX9149161.1"
                     /translation="MTRCDRNCGLCLLHSRERLPDAELIDRILDGETSMFEVLLHRYC
                     VHLLRVATAILGNDADAEDAVQQTCINAYVHLGGFERRSSLSTWLTAIAVNEARGRLR
                     PRRSTVVEQLDDREAARMRSMAPGPEEECMASELAARLRAGVEALPSQYRVVFDLRRN
                     QGLSTLEVADRLGLARDVVKTRLYRAKQKLRASIGAVN"
BASE COUNT          365 a          585 c          612 g          321 t
ORIGIN      
        1 agctacacag tattgccaca gcgaacgatc accacagaga cgcagaggac gcagagagac
       61 acagaggtga tcgcgtacgg cggagactct ctgtacgcag cgcatgaaac gcccaggccc
      121 ggatacatcc cttcccgtcc gcatcgggtt cgagctccag cgtcacgtaa cctttcacct
      181 cgccccgctc cgcggcactc gtcggttcgg tcgagcctgg atagaacacc ggcgcgccga
      241 ttgaccggcc cgacagatcg gccgtcagga gctgatggcg gtggatgtgc ccggcaagca
      301 cggccgcgaa gccgtggggg atggcccggc cggggatcgc gtcggcgttg gtgcgaaaga
      361 cataaccgac gggaccgacc gttgcgccct ccaccgtctg atgaaaacag aggagacgaa
      421 gatccgatgg aacggatctc catccggtcc gttcgacgag ccgggcgaac gtgtcgcgga
      481 cctcgtgacg ttcgcaggga aaaccggcta cggcgacatc gactccgcga atgttcagcg
      541 agatcgtgcg tggccggtcg aagagatgga tccgcgggtg cgcagcgaga attgggtacg
      601 gaatcgccga gcgctcgtgg ttccccggca cgacgaggac cggcactccg gagtcggcga
      661 tccgcttgag cggctcgaac gcgcgcatga ccagccccgg tttcacccgg ctccggaaaa
      721 agacgtcgcc tccgtgaacc acgaaatcca cttcgcctcg aaacgcgggc tgcaatgcgc
      781 gttcgaacgc gctgaagaaa tcctcgcctc tccggcggcg atcgctgcgc ggacgctcag
      841 gcagatcgaa tccaagatgg gtatcggcaa tgagcaggac tcggattcgt cgactcgaca
      901 cgagggaaag ggtaacgggc gggacttgct cctgtggcgc gaagagggga acgctccgtt
      961 gagatcgtgc gattagaacg ccgtaatcta cgattcgtgg atctcgtccc tgactcatcg
     1021 ccagctgtcg agctgggcgg cggccgatga cgtccggatc acggtggcgc cgatcttcca
     1081 gcgtcctcgc gccgttcgca tttttccctt ccgaacgaag aggagagcgg cggcgtgatc
     1141 tggctcactc caaatcgctt tcctggcatg tagcctgttt agcaacccag atattggata
     1201 acttcggagt gactgatagg aacctcgtgc tgacatgacc agatgtgatc gaaactgcgg
     1261 cctctgcctc ctccattcac gtgaacgcct gcccgatgcc gagctgatcg atcggattct
     1321 cgacggcgag acatcgatgt tcgaggttct cctccataga tactgcgtgc accttctccg
     1381 cgtggcgacg gcgattctcg gcaacgacgc agacgcggag gacgcggttc agcagacctg
     1441 catcaacgct tacgtgcatc tgggcgggtt cgagcgccgg tcgagcttgt caacctggct
     1501 cactgccatc gcggtgaacg aggctcgcgg gcgcctgcgt ccgcgccgct cgaccgtggt
     1561 cgaacagctc gacgacaggg aggccgcgag gatgcggtcg atggcgcccg gtcccgagga
     1621 ggagtgcatg gccagcgagc ttgcggcccg gctaagggcg ggcgtcgagg ctctgccgtc
     1681 gcagtaccgc gtcgtcttcg atctccgcag aaaccaaggg ctgtcgacgc tggaggtggc
     1741 cgaccgcctc gggctcgcgc gtgacgtcgt caagacacgc ctgtaccggg ccaagcagaa
     1801 actgcgcgcg agcatcggcg cagtcaactg aagcgttcgc gacgtcttcc gtggcccggc
     1861 tacgcagttt tggccacagg gaa
//
LOCUS       DASWAV010000402         3094 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U2665
            k141_542988, whole genome shotgun sequence.
ACCESSION   DASWAV010000402 DASWAV010000000
VERSION     DASWAV010000402.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960316
            Sequence Read Archive: SRR10260007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 3094)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3094)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:47:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,929
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3094
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_542988"
                     /isolate="SMAG_U2665"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.36444 N 132.31889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(14..1105)
                     /locus_tag="VF958_08390"
     CDS             complement(14..1105)
                     /locus_tag="VF958_08390"
                     /inference="COORDINATES: protein motif:HMM:NF024777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LamG domain-containing protein"
                     /protein_id="HEX9149162.1"
                     /translation="MPRVLQRQLHFPTRLFLAFVLVTLLLLLFSTVAAAQPFGAWPVF
                     TATQGKYVDVTNTADLNPPTALTIEFWVSNFPTAAQGEQCRSIIGKNYLTSYWVGICG
                     GIVRSRLHGTPHDGGVVPTGQWTHVAVTYDGVNRIHYINGEQVVVFPETGALPANTSN
                     LRIGSDVSWDYQPQGSVDEVRLWNYARSVEQIRGSINVQITSPQPGLIAVWNMDPGLT
                     DVIGGHNGTNIGGVPFLNFPVTLTCGSGTATTACLNTRFGVNITFRDPNTGMTGVGQV
                     VDCPNPDSALFWFFAPNAWEVMVKTVNACNFNDRYWVFSAAATNVFYRLEVVDVRAGA
                     AKIYFNYPGPPALAVTDTAAFETCIGVGP"
     gene            1286..1498
                     /locus_tag="VF958_08395"
     CDS             1286..1498
                     /locus_tag="VF958_08395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007304622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HEX9149163.1"
                     /translation="MSRYLIVIENAGSNYSAYSPDLPGCVTTGTTVEETKRKMEGAIE
                     LHLEGLREDGLPIPPPSTVAEYVEIA"
     gene            1751..1930
                     /locus_tag="VF958_08400"
     CDS             1751..1930
                     /locus_tag="VF958_08400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HEX9149164.1"
                     /translation="MKLQVVLEPTEEGGFTASVPSLPGCISEGDTREQALQNIREAIE
                     LYLEPADDNLAGKLS"
     gene            complement(2305..2487)
                     /locus_tag="VF958_08405"
     CDS             complement(2305..2487)
                     /locus_tag="VF958_08405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009567123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9149165.1"
                     /translation="MEKKVEKLSPRELSLFREWFATFDAEIWDRQIESDVAEGKLDRL
                     ADEAIAADKRGDSREI"
     gene            complement(2682..3047)
                     /gene="erpA"
                     /locus_tag="VF958_08410"
     CDS             complement(2682..3047)
                     /gene="erpA"
                     /locus_tag="VF958_08410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013292344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster insertion protein ErpA"
                     /protein_id="HEX9149166.1"
                     /translation="MTGSEPTGITEAKPFLSLTDAAVEKVKYFAKSMPDSEGKPLRIF
                     VSGGGCSGFQYGFQFDEKREDDNVLETGGVTVVVDPQSATYLRESTVDYVEDFRGAGF
                     SVTNPQATGGCGCGKSFSA"
BASE COUNT          587 a          966 c          988 g          553 t
ORIGIN      
        1 ccccgtccct cccctacggc cccacgccga tgcacgtctc gaacgccgcc gtgtccgtca
       61 cggccagcgc cggcggaccc ggatagttga agtaaatctt ggccgcgccc gcccggacgt
      121 cgactacctc gagccggtag aacacgttcg tcgccgccgc cgaaaaaacc cagtagcggt
      181 cgttgaagtt gcaggcgttg accgtcttga ccatcacttc ccacgcgttg ggcgcgaaga
      241 accagaagag cgcggaatcc ggattcgggc agtcgacgac ctggccgact ccggtcatgc
      301 cggtgttggg gtcgcggaac gtgatgttca cgccgaatcg cgtgttcagg cacgcggtcg
      361 ttgcggtgcc ggagccgcag gtgagcgtga cgggaaaatt cagaaacgga accccgccga
      421 tgttcgtccc gttgtgcccg ccgatgacgt cggtgagacc cgggtccatg ttccagaccg
      481 cgatcaggcc gggctgcggc gaggtgatct gaacgttgat cgacccgcgg atctgctcga
      541 cacttcgcgc gtagttccac aggcgcactt cgtcgaccga gccctgcggc tggtagtccc
      601 acgagacgtc gctgccgatg cggaggttcg acgtgttggc cgggagggca ccggtctcgg
      661 ggaagaccac gacctgctcg ccgttgatgt agtggatgcg gttgacgccg tcgtacgtga
      721 ccgcgacgtg cgtccactgg ccggtcggaa cgacgccgcc gtcgtgcggc gtgccgtgaa
      781 gccgggaccg cacgattccg ccgcagatcc cgacccagta cgaggtcagg tagttctttc
      841 cgatgatgct gcggcactgc tcgccctgag cagccgtcgg gaagttcgat acccagaatt
      901 cgatcgtcag cgcggtcggc gggttgagat cggccgtgtt cgtgacgtcg acgtacttgc
      961 cctgcgttgc ggtgaagacg ggccaggcgc cgaaaggttg tgccgccgca acggtggaga
     1021 agaggaggag caggagcgtg acgagaacga aagcgaggaa gagtcgggtc gggaagtgga
     1081 gctgccgctg aagtacccgt ggcatggtga cctccttgag caggcgggtt ggacctgcgg
     1141 gggagtctac tcccggaggg tcaggtctcc acttgccaca ttggacaaga tgtcattccg
     1201 aggagcgcag cgacgaggaa tctcgtccag atccctcgct tcgctcggca ggacagggat
     1261 aattggcagg cctgaagggg agatcatgag ccgctacctg atcgtcatcg aaaacgccgg
     1321 atccaactac tccgcatatt cacccgatct gcccgggtgc gtgaccacag ggacgacggt
     1381 cgaagagacc aagaggaaaa tggagggcgc gatcgagctc catctcgaag gccttcgcga
     1441 agacggcctg ccgattccgc cgccctcaac ggtcgcggaa tacgtcgaga ttgcgtagga
     1501 cgggtcgaga agtggggaac ccgcgtcgcg gctttcggaa gccgtcccct cacaacgcag
     1561 ccctgcggcc tccgaccgaa atcgacacgt caacgggaaa gggcgagtcg cgcctcgcgt
     1621 ccgggcggcc gcgacgattg gatcaacttc tttcgaaagc caccagacgg aatcgccatg
     1681 ccgaggtcga ctcagggccg cctgcaggtt gggaagtctg gtaattagtg cgggtctccc
     1741 gaaggaagaa atgaagttgc aggtcgtcct ggagccgact gaggaaggcg ggttcaccgc
     1801 ttcggtgcca tccctgcccg gctgcatcag cgagggggat acgcgggagc aagctctcca
     1861 gaacatccgc gaggcgatcg agctgtacct cgaacccgct gacgacaacc tcgccggaaa
     1921 gctctcctag cctgccattc caaggagcgc agccgagcgc caatctctcc ctcgaaggag
     1981 gacgagatcc cttgcttctc tcgggatgac agctgcgatc ggttcggtcg acagtggtgc
     2041 gttgcgttcg cgatcagccg accaggttgt cgtactcagc atgcgaaccg atccagaacc
     2101 acaggacttc gtcatcgaga ctcactccaa gcgcgcggtg tcgtctgccc acccgcaccg
     2161 accagaagcg accaatctcc ttgagatgaa gtgagggatg acggggattc tctctgagga
     2221 gatcgtagtt tcgccgggct tggctttgga tactcgcagg gagttggtcg aagaggcgcc
     2281 agaattccgg agcggcgcga tgcttcaaat ctcgcggctg tcgcccctct tgtcagcagc
     2341 gatggcttcg tccgcgagac ggtccagttt gccttccgcc acgtcactct cgatctggcg
     2401 atcccagatc tccgcatcga aagtggcgaa ccactcgcga aacagggaga gctccctcgg
     2461 tgaaagcttc tcgacctttt tctccacctc ttccacttta gtcatatctt gtcgcggacg
     2521 attttacggc ctacccgccc ctcaccccaa tcctctcccc gcgagcgggg agagggagca
     2581 tccggacatt ccctctcttc cggaggagag agtcagggtg agggaaaacg aaaagccccg
     2641 ctcgcgcggg gctttcaagg ccgaagaaga gtgccggcct gttacgcgct gaaagacttg
     2701 ccgcagccgc agccgccggt cgcctggggg ttcgtcaccg agaacccggc gccgcggaaa
     2761 tcctccacgt agtcgaccgt cgactcgcgc aggtacgtcg cgctctgcgg atcgacgacg
     2821 accgtgaccc cgcccgtctc gaggacgttg tcgtcctcgc gcttctcgtc gaactggaac
     2881 ccgtactgga acccggagca tccgccgccc gacacgaaga tccgcagggg cttgccctcg
     2941 gagtccggca tcgacttggc gaagtatttg accttctcga ccgccgcgtc cgtcagcgac
     3001 agaaacggct tggcctcggt gatcccggtc ggctcggagc cggtcatcgg ggattcgggg
     3061 acgatcgttg acgacattcc tacttactcc ttag
//
LOCUS       DASWAV010000403         2172 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U2665
            k141_340898, whole genome shotgun sequence.
ACCESSION   DASWAV010000403 DASWAV010000000
VERSION     DASWAV010000403.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960316
            Sequence Read Archive: SRR10260007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2172)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2172)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:47:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,929
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2172
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_340898"
                     /isolate="SMAG_U2665"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.36444 N 132.31889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1202
                     /locus_tag="VF958_08415"
     CDS             <1..1202
                     /locus_tag="VF958_08415"
                     /inference="COORDINATES: protein motif:HMM:NF020252.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="HDOD domain-containing protein"
                     /protein_id="HEX9149167.1"
                     /translation="DRTRALPRATLHSLLARTFTSGTNLTRLILEGRHDTLIGLSRRA
                     EEHARRLLFEVFEWRDAVFRYDPSRPVEKILRIRLRLRPQALAFQAVKNIDDARRLRR
                     RSTPSGFREALFEREELDRRFWDVVERAGDPVDAAEGRRRFAELVGFADRLRRRLGRI
                     EALRPIYEDSSALLRELLKRKPLEISAIEPIAALDPFLTLDLLILANSLVVSRSRSAA
                     TAHEALGRLGRRAVATLVERLAASEFPRVEEGDRAGTAVRRASIAAAVAASRRAAEFG
                     VPPERAYALGLLHTVPYADLLAVVEEMELSPGRFRAALIEVHRALVGRLRAEGWSLPL
                     DLEAVLTDDGTNTRPAAALVRAARATLPDCALGPLLPAVVSAREARAAAVEVNRLFAF
                     LALPPAG"
     gene            1301..>2172
                     /locus_tag="VF958_08420"
     CDS             1301..>2172
                     /locus_tag="VF958_08420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HEX9149168.1"
                     /translation="MKPIAALTATLLSLSPALVAAQERPPTFTEEVEVRVMDLDVVVT
                     DKGGRPVPDLKREDFTVRIGGKPVPIDYFARVDEGTIHAPDLATASPDQVLAAYRRGD
                     EAFVPRHFLMYVDIGHLAPDARKRGLEALRDLVTRMGPNDMGRVLMFDRRSKELTEWT
                     SNKEQLFLALTKIEKAGVGMSRLLTERQTLRDIDSTRSRSSRGFLARQYAEQERAEVR
                     QMLDDVGSELATLAPLAGKKAFLFVSGGFEFQPGYAMATYALGQPSLLALDVRNVGPE
                     LEALARRANASEIT"
BASE COUNT          307 a          774 c          736 g          355 t
ORIGIN      
        1 tggaccgcac gcgggcgttg cctcgcgcga cgcttcactc gcttctcgca cggactttca
       61 cctcgggcac gaacctgacg cggctcatcc tcgaggggcg acacgacact ttgatcggtc
      121 tctcccgccg ggccgaggag cacgcccgcc ggctgctgtt cgaggtgttc gagtggcgcg
      181 acgcggtgtt tcggtacgac ccctcgcggc cggtcgagaa gatcctccgc atccggctcc
      241 ggcttcgacc ccaggcgctc gcctttcaag cggtcaagaa catcgacgac gcgcgtcggt
      301 tgcgccggcg ctccacgccg agcgggtttc gcgaggcgct cttcgagcgc gaggagctcg
      361 atcgacgttt ctgggacgtc gtcgagcggg ccggcgaccc cgtggatgcc gcggaaggcc
      421 gccggcgctt cgccgagctc gtgggtttcg ccgaccgcct ccgccgccgg ctcgggcgga
      481 tcgaggccct gcggccgatc tacgaggatt cctccgccct cctccgcgag ctcctgaagc
      541 ggaagcccct cgagatctcg gcgatcgagc ccatcgccgc cctcgacccg ttcctgaccc
      601 tcgacctact cattcttgcg aacagtctgg ttgtcagccg ctcgcgttcc gccgcgacgg
      661 cgcacgaagc gctcgggcgt ctgggacggc gcgccgtcgc gactctcgtc gagcggctcg
      721 cggcctcgga gtttccacga gtcgaggagg gggaccgcgc cggtaccgcg gtgcgccggg
      781 cgagcatcgc agccgcggtc gccgcatcga ggcgagcggc ggagttcgga gttcctcccg
      841 agcgggccta cgccctggga ctcctgcaca cggtgccgta cgcggatctg ctcgctgttg
      901 tcgaagagat ggagctctct ccggggcggt tccgcgcggc gctcatcgaa gtccatcgcg
      961 cgctcgtcgg ccggctgcgc gcggagggat ggagcctgcc gctcgatctc gaggcggtgc
     1021 tcaccgacga cggcacgaac acgcgccccg cggcggccct ggttcgggcc gcgcgtgcga
     1081 cgctgccgga ctgcgctctc ggaccgctct tgccggcggt cgtttccgcg cgcgaagcgc
     1141 gggccgcggc cgtcgaggtg aaccggctct ttgctttcct ggcgcttccc ccggcggggt
     1201 gagatggggc tcggtccgaa cattaagggc agcttcatcc gcggcctccg aggaatcgcc
     1261 ccgggcccgc tcgcgtttgg gacttccgag gatgaagaag atgaaaccga tagctgcact
     1321 gaccgccacc ctgctctcgc tctcccccgc gctcgtcgcc gcccaggaac gcccgccgac
     1381 gttcaccgag gaggtcgaag tccgggtcat ggatctcgac gtcgtcgtga ccgacaaggg
     1441 gggccgtccg gtcccggact tgaagcgcga ggacttcacc gttcgaatcg gcggcaaacc
     1501 cgttccgatc gactacttcg cacgcgtgga cgagggaacg atccacgcgc cggatctcgc
     1561 gaccgcttct cccgatcagg tcctcgccgc atatcggcgc ggagatgaag cgttcgtccc
     1621 gcgccatttc ctcatgtacg tcgacatcgg acacctcgcg ccggacgcgc gcaagcgcgg
     1681 gctcgaggcg ctgcgcgatc tcgtgacccg catggggccg aacgacatgg gccgggtgct
     1741 gatgttcgat cgccgctcga aggagctcac ggagtggacc agcaacaagg agcagctctt
     1801 ccttgcgctg accaagatcg agaaggcggg cgtcgggatg tcgcgcctcc tgaccgaacg
     1861 tcagacgctg cgggacatcg actccactcg cagccggtcg agccgtgggt tcctggcgag
     1921 gcaatacgcc gagcaggagc gggcggaagt gcgccagatg ctggacgacg tcggctccga
     1981 gctcgcaacg ctcgcgccgc tcgccggcaa gaaggccttc ctcttcgtct ccggcggctt
     2041 cgagttccag ccgggctacg cgatggcgac ctacgctctc ggccagccga gtctcctcgc
     2101 tctcgacgtg cggaacgtgg gacccgagct cgaggccctc gcgcgccggg cgaacgcctc
     2161 cgagatcacc tt
//
LOCUS       DASWAV010000404         2182 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U2665
            k141_261721, whole genome shotgun sequence.
ACCESSION   DASWAV010000404 DASWAV010000000
VERSION     DASWAV010000404.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960316
            Sequence Read Archive: SRR10260007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2182)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2182)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:47:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,929
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2182
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_261721"
                     /isolate="SMAG_U2665"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.36444 N 132.31889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..983
                     /locus_tag="VF958_08425"
     CDS             <1..983
                     /locus_tag="VF958_08425"
                     /inference="COORDINATES: protein motif:HMM:NF022588.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF2911 domain-containing protein"
                     /protein_id="HEX9149169.1"
                     /translation="QPALNLPQPSPKATISQTVGVTEIAITYHRPAVNKRTVWGDLVP
                     YDQVWRAGANENTTIAFSSRVTVNGKPLAAGTYGIHMIPAKSGDWTIAFSNVSWAWGS
                     FTYDEKEDALRVTAKPQPAEFQERLSYGFDDPTSRSVDVALRWEKVRIPFTVEVDTPA
                     VVVESLRKELRDLPRFSWQGWNQAAAYCLQSNVNAEEALAWADRSIRMQENFNNLRTK
                     AGLLELKGDTKAAADLRDRAMKIATEADINGYGYQLLGAGKTDEAIEIFRKNVKDHPQ
                     SWNVYDSLGEAYDRKGDRKLAVENYRRAQNMTQDPDQKKRISDILARLNG"
     gene            complement(984..>2182)
                     /locus_tag="VF958_08430"
     CDS             complement(984..>2182)
                     /locus_tag="VF958_08430"
                     /inference="COORDINATES: protein motif:HMM:NF013799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HEX9149170.1"
                     /translation="FSPARKIVLGFEKTSHSVAFIALDATDDGRVDYDRLLVLPESGG
                     RGEVRVGEWFPVEVPNEEGRTGAWCKLLALAPDLASAEIYVGPLSRSTGAPREWVRRL
                     DEEIGFWPGMPDTDAFGEDSSGPENFLEQTDRLVDFLTRADLLALSRRDWDLLLIYQP
                     EVDETSHEFLLVDPAQSRFTPERSARFVGFVEKTYALAERSLDAIEKALTPSDSLFVT
                     SDHGMTPIFGDIAPNRILRDAGLVTVDGKDKVTTSSPLVAVTASGIAHVYVNPAADRS
                     VLDRAERVLNEFRVRGENPFDRVVRREHAGELGLNAPESGDLIVLAKPGFQFVSDAGG
                     APLRESHSYGGHGYRNVYPDLDATFFAVGPGIAHERVEEIGSWQIAARVARALGIAPP
                     RQAAAP"
BASE COUNT          410 a          725 c          726 g          321 t
ORIGIN      
        1 cgcagcccgc gctcaatctg ccccagccga gtcccaaggc cacgatttcg cagaccgtcg
       61 gggtgacgga gatcgcgatc acctatcacc gaccggctgt caataaacgt acggtgtggg
      121 gcgacctcgt cccctacgac caggtctggc gcgcgggagc caacgagaac acgacgatcg
      181 cgttctcctc tcgggtcacg gtcaacggca agcctcttgc cgccggcacg tacgggatcc
      241 acatgatccc ggcgaagagc ggcgattgga cgatcgcgtt ttcgaacgtg agctgggcat
      301 ggggcagttt cacttacgac gagaaggaag acgccctccg ggtcaccgcg aagccgcagc
      361 cggccgaatt ccaggagcgc ctctcgtatg gtttcgacga ccccacctcc cgctccgtgg
      421 acgtcgcgct ccgatgggaa aaggtgcgca tcccgttcac cgtggaggtc gacacgccgg
      481 cggtcgtcgt cgagagcctt cgcaaagagc ttcgcgacct cccccgcttt tcctggcagg
      541 ggtggaacca ggcggccgcg tactgcctcc agagcaacgt gaacgccgag gaggcgctcg
      601 cgtgggccga tcgctcgatc cggatgcagg agaacttcaa caacctgcgc acgaaggccg
      661 ggctcctcga gctcaagggg gacacgaagg ccgccgcgga cttgcgggat cgcgcgatga
      721 agatcgcgac cgaggcggac atcaacggtt acgggtacca gctgctgggc gcgggcaaga
      781 ccgacgaggc gatcgaaatc ttccgcaaga acgtcaagga ccacccccag agctggaacg
      841 tctacgacag cctcggcgag gcgtacgaca ggaaggggga ccggaagctc gcggtcgaga
      901 actaccgccg cgcgcagaac atgacccagg atcccgacca gaagaagagg atcagcgaca
      961 tcctcgcccg cttgaacggt tgatcagggg gccgcggcct gtcgcggggg cgcgatcccg
     1021 agcgccctcg cgacacgagc cgcgatctgc caggagccga tctcttcgac gcgctcgtgc
     1081 gcaatccccg gaccgaccgc gaaaaacgtc gcgtcgaggt cagggtagac gtttcggtag
     1141 ccgtggccgc cgtagctgtg ggactcccgg agcggagcgc cccccgcgtc gctgacgaat
     1201 tggaaccccg gtttcgcgag cacgatcagg tccccagact ccggagcgtt caggccgagc
     1261 tcgcccgcgt gctcccggcg gacgacccgg tcgaacggat tttccccccg gacgcggaat
     1321 tcgttcaaca ctcgctctgc ccggtcgaga accgatcgat ccgcggccgg gttcacgtaa
     1381 acgtgcgcga tgccgctggc ggtaaccgcg accaacggag aggacgtcgt gaccttgtcc
     1441 tttccgtcga ccgtgacgag gcccgcgtcg cggaggatcc ggttgggcgc gatgtccccg
     1501 aagatcggag tcatcccgtg gtccgaggtg acgaagagcg agtcggacgg cgtgagcgcc
     1561 ttctcgatgg cgtcgagcga gcgttcggcg agtgcgtagg tcttctcgac gaagccgacg
     1621 aagcgagccg agcgctcggg cgtgaagcgc gactgcgcgg ggtcgacgag cagaaactcg
     1681 tgtgacgttt cgtcgacctc cggctgatag atgagaagga ggtcccagtc gcgccgggag
     1741 agcgcgagaa ggtccgcgcg cgtcaggaaa tcgaccagcc gatcggtctg ctcgagaaaa
     1801 ttttccggac cggaactgtc ctcgccgaac gcgtcggtat cgggcatgcc gggccagaag
     1861 ccgatctctt cgtcgagccg gcgcacccac tcgcgcggcg ctccggtcga gcgggaaaga
     1921 ggcccgacgt aaatctccgc gctcgccaga tcgggcgcga gcgcgagcag cttgcaccac
     1981 gcgccggtgc gaccctcctc gttcgggact tcgaccggaa accactctcc cacccgcacc
     2041 tcgccgcgcc ctccggattc cgggaggacg agcagccggt cgtagtccac gcgtccgtcg
     2101 tcggtcgcgt ccagcgcaat gaacgcgacg gagtgcgacg tcttctcgaa cccgagcacg
     2161 atcttccggg ccggcgagaa ac
//
LOCUS       DASWAV010000405         2273 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U2665
            k141_543347, whole genome shotgun sequence.
ACCESSION   DASWAV010000405 DASWAV010000000
VERSION     DASWAV010000405.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960316
            Sequence Read Archive: SRR10260007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2273)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2273)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:47:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,929
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2273
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_543347"
                     /isolate="SMAG_U2665"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.36444 N 132.31889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>2273
                     /gene="carB"
                     /locus_tag="VF958_08435"
     CDS             <1..>2273
                     /gene="carB"
                     /locus_tag="VF958_08435"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013906887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HEX9149171.1"
                     /translation="GAEVPRSERARSLEDAERIAREIGLPLVVRPSFTLGGSGGGAAR
                     TIGEFRSIVRHGLAESPAHQVLIEESVEGWKEFELEVMRDHADNVIVVCSIENFDAMG
                     VHTGDSITVAPQMTLSDRQYQEMRNEAKAIIRKIGVDTGGSNIQFAVEPSTGRRVVIE
                     MNPRVSRSSALASKATGFPIAKIAALLAVGYTLDEIPNDITRKTPACFEPSLDYVVVK
                     IPRWAFEKFPKADPTLTIQMKSVGEAMAIGRTFAEALGKAIRSLEIGRDGLDDGRGPL
                     PPEELLAKLEVPTWERIFHLRRAFLAGMTVDEVAASTRIDPWFLDVIRRMVGEETCLA
                     GRRIDALSRDELRKSKRLGLSDSAIGRAMGVAEGAVRRRRRELGVAPVYKTIDTCAAE
                     FEAYTPYYYSTYEDENESLRTEKPKVVILGGGPNRIGQGIEFDYCCVHACYALADAGY
                     ETVMINCNPETVSTDYDTSDRLYFEPLTFEDVSAVLDNERPDGVLVQFGGQTPLKLAR
                     PLEAAGYPIWGTSPESIDLAEDRGRFGKLLGELGLEAPPHGEARTADEALGIARRIGY
                     PLVVRPSYVLGGRAMAIVFDDDHLLSYMTRALEAAPEHPILIDRFLDDAFEFDVDALS
                     DGKTTWIGGIQEHIEKAGIHSGDSFSVLPAWKVPPEKLREMADATRRLAEALDVRGLM
                     NIQFATQGGILYVLEVNPRASRTVPFVSKAIGVPMARAAALLAAGRPLESLGLPPERQ
                     PVDYFIKAPVFPFRKFP"
BASE COUNT          361 a          755 c          804 g          353 t
ORIGIN      
        1 tcggggccga ggtccctcgc tccgagcgcg cgcgcagcct cgaggacgcc gagcgcatcg
       61 cgcgggagat cgggctgccg ctcgtcgtcc gcccttcgtt cacgctgggc ggatcgggcg
      121 gcggtgcggc gcggacgatc ggcgagttcc gctcgatcgt tcggcacggc ctggcggagt
      181 cgccggcgca ccaggtgctg atcgaggaat ctgtcgaggg gtggaaggag ttcgagctcg
      241 aggtcatgcg cgaccatgcg gacaacgtga tcgtcgtgtg ctcgatcgag aacttcgacg
      301 cgatgggcgt tcacacgggc gactcgatca cggtcgcgcc gcagatgacg ttgtcggacc
      361 ggcagtacca ggagatgcgc aacgaggcga aggccatcat ccgcaagatc ggcgtcgaca
      421 cgggcggctc gaacatccag ttcgcagtcg agccctcgac cggccggcgc gtcgtcatcg
      481 aaatgaatcc gcgcgtctcg agatcttcag cgctcgcgtc gaaggcgacg gggttcccga
      541 tcgcgaagat cgccgcgctc ctcgccgtgg ggtacacgct cgacgagatc cccaacgaca
      601 tcacccgcaa gacgcctgcc tgcttcgaac cgtcgctcga ctacgtcgtc gtgaagatcc
      661 cgcgttgggc cttcgagaag ttcccgaagg ccgacccgac gctcacgatc cagatgaagt
      721 cggtcggcga ggcgatggcg atcggacgga cgttcgccga ggcgctcggc aaggcgatcc
      781 ggtcgctcga gatcgggcgc gacggcctgg acgacgggcg cgggccgctg ccgcccgagg
      841 agctgctcgc gaaactcgag gtcccgacgt gggagcggat ctttcacctg cgccgggcgt
      901 tcctcgcggg gatgactgtg gacgaagtcg cggcgagcac tcggatcgac ccctggtttc
      961 tcgacgtgat ccgcaggatg gtcggtgagg agacgtgcct cgcgggtcgg cggatcgacg
     1021 cgctctcgcg ggacgagctc cggaagtcga agcggctcgg cctctcggat tcggcgatcg
     1081 ggcgcgccat gggcgtcgcc gaaggcgccg tccgccggcg ccgcagggag ctcggggtcg
     1141 cgccggtgta caagacgatc gacacgtgcg ccgcggagtt cgaggcgtac acgccgtact
     1201 actactcgac ctacgaggac gagaacgagt cgttgcggac ggagaagccg aaggtcgtca
     1261 tcctgggggg cggcccgaac cgcatcggcc aggggatcga gttcgactac tgctgcgtgc
     1321 acgcgtgcta cgcgctcgcc gacgccggat acgagaccgt gatgatcaac tgcaacccgg
     1381 agacggtttc gacggactac gacacgtccg accggctcta cttcgagccg ctgacgttcg
     1441 aggacgtgtc cgctgtcctc gacaacgaga gaccggacgg cgtcctcgtc cagttcggcg
     1501 ggcagacgcc gttgaagctc gcgcggccgc tcgaggccgc gggatacccg atctggggga
     1561 cgtcccccga gtcgatcgac ctcgcggagg accgcggccg gttcggaaag ctgctcggag
     1621 agctcggcct cgaggcgccg ccccacggag aggcacggac ggccgacgag gcgctcggca
     1681 tcgcgcggcg gatcggctat ccgctcgtcg tgcggccctc ctacgtcctc ggcggccgcg
     1741 cgatggcgat cgtcttcgac gacgaccacc tcctttcgta catgacccgc gcgctcgagg
     1801 ctgcgccgga acacccgatc ctgatcgacc gtttcttgga cgacgcgttc gagttcgacg
     1861 tggacgccct ctccgacggg aagacgacgt ggatcggggg catccaggaa cacatcgaga
     1921 aggccggaat tcactcgggc gactcgttct ccgtgctgcc ggcctggaag gtcccgcccg
     1981 agaagctccg cgagatggcg gacgcgacga gacggctggc cgaggcgctc gacgtccggg
     2041 gcctcatgaa catccagttc gcgacgcagg gcgggatcct ctacgtcctc gaggtgaatc
     2101 cccgggcttc gcgcacggtg cccttcgtct ctaaggcgat cggcgttccg atggcgcgcg
     2161 cggcggcgct cctcgcggcg gggcggccgc tcgagtcgct cgggctcccg ccggagcggc
     2221 agcccgtcga ctacttcatc aaggcgccgg tctttccttt ccgcaagttc ccg
//
LOCUS       DASWAV010000406         3126 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U2665
            k141_462711, whole genome shotgun sequence.
ACCESSION   DASWAV010000406 DASWAV010000000
VERSION     DASWAV010000406.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960316
            Sequence Read Archive: SRR10260007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 3126)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3126)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:47:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,929
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3126
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_462711"
                     /isolate="SMAG_U2665"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.36444 N 132.31889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1416)
                     /locus_tag="VF958_08440"
     CDS             complement(<1..1416)
                     /locus_tag="VF958_08440"
                     /inference="COORDINATES: protein motif:HMM:NF017745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF885 domain-containing protein"
                     /protein_id="HEX9149172.1"
                     /translation="MALVSAAPAPAGSGLRALLDEVWEFELREDPIFATSVGDHRYDD
                     RLPAVGIADQARRADARRKFLERLKAIDQSGLDETDRDSRALQRVTLEDAIREFDLHH
                     YRFPINADSGFHMGFAQLVNQIPLDTTHDYENYIARLRAFPEYVRGNVELLREGLQTG
                     WTLPKAALAGYEKTIEAHVVADPARSRFFVPFASFPAGVPEGDRTRLRAAGEKAIRDD
                     VVPAYRAFLEFMTREYMPGARATVGAGDLPGGKEYYAYLVRHFTTLDRTPDEIHALGL
                     EEVRRIRAEMEEVIRKTGFSGGFADFLKFLRTDPRFYAKTPEELLKQASWIAKRIDGK
                     LPSLFGRLPRQPYGVEPVPAEIAPKYTGGRYVGAPPGGKKAGIYWVNTYALESRPLYV
                     LESLTLHEAVPGHHLQGALSAELTDLPPFRRFLYVDAFGEGWGLYSERLGLEMGLYTD
                     PYSNFGRLTYEMWRACRLVVDT"
     gene            complement(1452..>3126)
                     /locus_tag="VF958_08445"
     CDS             complement(1452..>3126)
                     /locus_tag="VF958_08445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9149173.1"
                     /translation="RRIPAVPAGTPTVQRLAVLPFENLGSPQDDYFADGIADEIRAKL
                     TALSGVEVIARGSSTPYKKTTKSQTKIAGELGVGYLLTATVRWQKSAGASRVQVTPEL
                     VEIRETGAPASKWQESFDAALTDVFQVQSDIASQVATALGVALRGREEQRLLEKPTRN
                     LAAYDAFLKGQEIWRSLAVGDPPALRRALSFYDQAVALDPTFAQAWARISWANSTLYM
                     NSSPTPDVSGRAREAAEKAVALAPDAPEGYHALGNYRRLVLKDYGGALEQYTKGQQLS
                     PGNADLLTATALAEESLGRWAAAVDHLRQAEHLDPRSIPQRLRLGQALRYLRRYPEAR
                     QALDRGLELAPVNLALISEKVMTFLGEGDLAGARKVVAAAPKEVEPTALVAYLANVDL
                     VWILDGGQREVLLRLTPGAFSDDRAAWALALLQAEADAAKRRDYAGVAVKALEEQLRS
                     APDDALRRSALGVALMRLGRREEAIHEGERSVALAPVAKDYQIGAFIQHQLALIDILA
                     GEPEKALDNLEPLLKIPYHLSPGWLKIDPNFDSLRDNPRFQKLVGGNSL"
BASE COUNT          510 a         1027 c         1075 g          514 t
ORIGIN      
        1 cgtgtccacg acgagccggc acgcgcgcca catctcgtag gtcagacgcc cgaagttgct
       61 gtacggatcg gtgtagagac ccatctccag gccgagccgc tccgagtaga gtccccagcc
      121 ctcgccgaat gcgtccacgt agaggaagcg ccggaagggg ggcagatccg tcagctccgc
      181 cgagagcgcg ccctggaggt ggtgtcccgg gaccgcctcg tgaagcgtga gtgactcgag
      241 gacgtagagc ggccggcttt cgagcgcata ggtgttgacc cagtagatcc cggccttctt
      301 gccgcccggc ggggcgccga cgtagcggcc gccggtgtac ttgggcgcga tctccgcggg
      361 gacgggctcg acgccgtagg gctggcgcgg caggcgtccg aagagcgagg gcagttttcc
      421 gtcgatgcgc tttgcgatcc acgacgcctg cttcaagagc tcctcgggag tctttgcata
      481 gaagcgcggg tcggtgcgca ggaacttcag gaagtcggcg aatccgccgg aaaacccggt
      541 cttgcggatg acttcttcca tctcggcacg gatgcggcgg acctcttcga ggccgagggc
      601 gtggatctcg tcgggcgtgc ggtcgagcgt cgtgaagtgc cggacgagat acgcgtagta
      661 ctccttcccg cccggcagat cacccgcgcc caccgtcgcg cgcgcgccgg gcatgtactc
      721 gcgggtcatg aactccagga acgcgcgata cgccggaacg acgtcgtcac ggatcgcctt
      781 ctccccggcg gcccgcaggc gggtgcggtc gccctcgggc acgccggccg gaaaggaagc
      841 gaacggaacg aagaaacggc tgcgggcggg atccgcgacg acgtgcgcct cgatcgtctt
      901 ctcgtagccg gcgagcgccg ccttcggaag cgtccagccc gtctgcaatc cctcgcgcag
      961 gagctcgacg tttcctctca cgtactccgg gaaagcccgc agccgcgcaa tgtagttctc
     1021 gtagtcgtga gtcgtgtcga gcgggatctg gttgacgagc tgggcgaagc ccatgtgaaa
     1081 accgctgtcg gcattgatcg gaaagcggta gtggtggagg tcgaactcgc ggatcgcgtc
     1141 ttcgagcgtc acgcgctgga gggcccggct gtcgcgatcc gtttcgtcga gcccggactg
     1201 gtcgatcgct ttcaggcgct cgaggaactt ccggcgcgca tcggcgcggc gtgcttggtc
     1261 cgcaatgccc accgcgggaa gccggtcgtc ataacggtgg tcgccgacgg acgtcgcgaa
     1321 gatcggatcc tcccggagct cgaactccca gacttcgtcg agcaaggctc gaagacccga
     1381 tccggccggg gccggcgcgg ccgagacgag agccatcgat ccgagaacgg cgcagagaag
     1441 agtgttggcg atcaaagcga gttccctccg acgagtttct gaaaccgcgg attgtctcgc
     1501 agcgaatcga agttcgggtc gatcttcagc caaccggggg agaggtgata cgggatcttc
     1561 aggagcggct ccagattgtc gagcgccttc tccggctcgc cggcgaggat gtcgatcagc
     1621 gcgagctggt gctgaatgaa ggcaccgatc tggtaatcct tcgccaccgg agccagcgcc
     1681 acgctgcgtt ctccttcgtg gatggcctcc tcgcggcgac cgaggcgcat gagcgcaacg
     1741 cccagcgcgg agcggcgcag cgcgtcatcg ggagccgaac gcaactgttc ctccaacgcc
     1801 ttcacagcaa ctcccgcata gtctcggcgc ttcgcggcgt cggcctcggc ctggagaagc
     1861 gcgagcgccc aagcggcccg gtcgtcggag aaggcgcccg gcgtcaggcg aaggagaact
     1921 tcgcgctgtc ctccgtcgag aatccagacg agatccacgt tcgcgaggta agcaacgaga
     1981 gccgtcggtt cgacctcctt cggcgccgcc gcgacgacct tcctggcgcc ggcgagatcc
     2041 ccttcgccga ggaaagtcat caccttctcg ctgatcaggg cgagattcac cggcgcgagt
     2101 tcgaggcccc ggtcgagcgc ctgccgcgcc tcggggtagc gccggaggta gcggagcgcc
     2161 tggcccagcc tgaggcgctg tggaatcgac cgcgggtcga gatgctcggc ctgacggagg
     2221 tggtcgaccg ccgccgccca acgaccgaga gactcctcgg ccagcgcggt cgccgtcagg
     2281 aggtcggcgt ttcccggtga gagctgctga cccttcgtgt actgctcgag cgcacctcca
     2341 tagtccttga gtaccagtct ccgatagttg cccagagcgt gatacccctc cggagcgtcg
     2401 ggagcgagcg ccaccgcctt ctccgcggcc tcgcgcgcgc gcccggacac gtcgggagtc
     2461 ggcgaactgt tcatgtagag ggtcgagttg gcccacgaaa tccgcgccca cgcctgggca
     2521 aaggtggggt cgagcgcaac ggcctgatcg tagaaggaca acgccctccg cagggcggga
     2581 gggtctccga cggccaggct cctccagatc tcctggcctt tcaggaacgc gtcgtaggcc
     2641 gccaggtttc gtgtcggctt ctcgaggagg cgttgctcct ctctccctcg aagcgcgacg
     2701 ccgagcgccg tggccacctg ggaagcgatg tccgactgca cctggaagac gtcggtcagc
     2761 gccgcgtcga acgactcctg ccacttcgag gcgggtgctc cggtttcccg gatttcgacg
     2821 agctcgggag tcacttgcac gcggctcgcc ccggcgctct tctgccatcg gacggtcgcc
     2881 gtgaggaggt agccgacacc gagctcgccg gcgatcttcg tctgcgactt cgtcgtcttc
     2941 ttgtacggcg tggagctccc gcgcgcgatc acctcgacgc ccgagagagc cgtgagcttc
     3001 gcccgaatct cgtccgcgat gccgtcggcg aagtagtcgt cctgcggaga acccaggttc
     3061 tcgaagggca gcacggcgag gcgctggacg gtcggagtcc ctgccgggac cgcgggaatg
     3121 cggcgc
//
LOCUS       DASWAV010000407         1909 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U2665
            k141_661583, whole genome shotgun sequence.
ACCESSION   DASWAV010000407 DASWAV010000000
VERSION     DASWAV010000407.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960316
            Sequence Read Archive: SRR10260007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 1909)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1909)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:47:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,929
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1909
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_661583"
                     /isolate="SMAG_U2665"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.36444 N 132.31889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1902
                     /gene="glyS"
                     /locus_tag="VF958_08450"
     CDS             <1..1902
                     /gene="glyS"
                     /locus_tag="VF958_08450"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: protein motif:HMM:TIGR00211.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009345 - glycine-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HEX9149174.1"
                     /translation="VPERQPDRETEVLGPPAAAAFDAAGQPTRAAEGFARAQKVEVSD
                     LVVVDSPRGKTVAARRTVAGRSASELLGEVVPRVVDGLSFPTTMRWGAGERSFVRPVR
                     GVLALYGGDVVPMDVMGMRAGDSTAGHRVLSDGDFSVSTPEEYFQKLRGAWVEPDGEA
                     RRAAILESARRLAAQVGGRIEADADLAAVLADLVEWPGLVRGSFDAQFLELPEEITTT
                     AMRVHQKFLPVRGADGLMPYFIAVMDNAMDRRGFIATGNEWVLNARLADARFFFKEDS
                     RETLESRLPALERLNFQDRLGSYAAKAGRLEDLAAAIAEAVKRLDIVSDVRTAAKLSK
                     ADLTSRMVREFTDLQGVMGGIYARAEGHPEAVWKGIYDHYRPQSQGDDPPREDSGAIV
                     SLADRFDSLAGLFRLGLLPTGSKDPYGLRRAAAGAVAIVISRKWRNDWSGIARKALEL
                     YPKDLPGLAPDRALAELEAFFAERLRNAIESAGSSYDEASAVLNVGAWNFADAAERAA
                     ALTGARRRLDFRSLVLAFKRIRNILVEGETQPEPSPTLYREGAERALASDFLQAKTLS
                     AELVKGRRYSEALETIASIAPSLDRFFVEVLVNVPEEDVRRNRHALLGAIRREFTKIA
                     DFSEIVVEK"
BASE COUNT          281 a          633 c          693 g          302 t
ORIGIN      
        1 gtgcccgagc gccagccgga ccgcgagacg gaagtgctcg gaccgccggc ggcggcggcg
       61 ttcgacgccg caggccagcc gacgcgagcc gccgagggct tcgcgcgggc acagaaagtg
      121 gaggtctccg atctcgtcgt cgtcgactcg ccccgcggca agaccgtcgc cgcgcgtcgg
      181 accgtcgcgg gccgatcggc gtccgagctg ctcggggagg tcgttccgcg ggtggtcgac
      241 ggactctcgt tcccgacgac gatgcgctgg ggcgccggcg agcgctcgtt cgtgcggccg
      301 gtgcgcggcg tgctcgcact ctacggcggg gacgtcgttc cgatggacgt gatggggatg
      361 cgcgcgggcg actcgaccgc cggccaccgg gtgctctccg acggcgattt ttccgtctcg
      421 acgccggagg aatactttca gaagctccgg ggtgcatggg tcgagcccga cggcgaggcg
      481 cggcgcgcgg cgatcctcga gtccgcgcgg cgtctggctg cgcaggtcgg aggacgcatc
      541 gaagccgatg ccgatctcgc cgcggtgctg gcggacctcg tcgagtggcc gggtctcgtc
      601 cggggctcct ttgacgcgca gttcctggag ctgcccgagg agattacgac gaccgcgatg
      661 cgcgttcacc agaagtttct tccagtgcgg ggcgccgacg gcctgatgcc gtatttcatc
      721 gcggtgatgg acaacgccat ggaccgccgg ggattcatcg ccacgggaaa cgagtgggtg
      781 ttgaacgccc gcctcgccga cgcgcgcttc ttcttcaagg aagactcgcg cgagaccctc
      841 gagtcgcgtc tccccgcgct cgaaaggctg aacttccagg atcggctcgg cagctacgcc
      901 gcgaaggcag gtcgcctcga ggacctcgcg gccgcgatcg cggaggcggt caagcggctc
      961 gacatcgtgt ccgacgtccg gaccgcggcg aagctctcga aggcggacct gacgagccgg
     1021 atggtccggg agttcacgga cctccagggg gtgatgggcg ggatctacgc gcgcgcggag
     1081 gggcatcccg aagcggtgtg gaaggggatt tacgaccact accggccgca gagccagggg
     1141 gacgatcctc cccgcgagga ttccggggcg atcgtctccc tcgccgaccg tttcgactcg
     1201 ctcgccgggc tcttccgcct ggggctgctt cccaccggct cgaaggatcc gtacggcctt
     1261 cggcgcgcgg cggcgggagc ggtcgcgatc gtgatttcgc gcaagtggag gaacgactgg
     1321 tccgggatcg cgcggaaggc tctcgagctt tatccgaagg atctgcccgg actcgcgccc
     1381 gaccgcgcgc tcgccgagct cgaggctttc ttcgccgaga ggctgcgcaa cgcgatcgaa
     1441 agcgccggct cctcctacga cgaagcgtcg gcggttctga acgtgggagc gtggaacttc
     1501 gccgacgcgg ccgagcgcgc cgcggcgctg accggcgcgc gccgacggct cgatttccga
     1561 tcgctcgtgc tcgccttcaa gcggatccgc aacatcctcg tggagggcga gacccagccc
     1621 gagccgtcgc cgactcttta ccgggagggg gccgagcggg cgctcgcatc ggacttcctc
     1681 caggcgaaga cgctctccgc ggagctcgtc aaaggacggc gctattccga ggcgctcgag
     1741 acgatcgcgt cgatcgcccc atcgctcgac cggttcttcg tcgaagtcct ggtcaacgtg
     1801 cccgaggagg acgtgcgccg gaaccggcac gccctgctcg gcgccatccg gcgggagttc
     1861 acgaagatcg cggatttttc tgagatagtc gtggagaaat gacatggtg
//
LOCUS       DASWAV010000408         1711 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U2665
            k141_22627, whole genome shotgun sequence.
ACCESSION   DASWAV010000408 DASWAV010000000
VERSION     DASWAV010000408.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960316
            Sequence Read Archive: SRR10260007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 1711)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1711)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:47:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,929
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1711
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_22627"
                     /isolate="SMAG_U2665"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.36444 N 132.31889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..377
                     /locus_tag="VF958_08455"
     CDS             <1..377
                     /locus_tag="VF958_08455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012161821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="VCBS repeat-containing protein"
                     /protein_id="HEX9149175.1"
                     /translation="LAVGDINRDGDEDLAVANQFCCGTDVSGPALSILSGNGIGGFAS
                     PSNFAVGTPVFVPGPFAVVLADFNRDGQLDVALGDSGSRIWIYLACAAVRADIPTLGP
                     LGLIVMGLLVALVALSRLNARG"
     gene            425..>1711
                     /locus_tag="VF958_08460"
     CDS             425..>1711
                     /locus_tag="VF958_08460"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HEX9149176.1"
                     /translation="MLYRFDRVRKSYGSKEVLREVTWQHNPGEKVGLVGRNGAGKTTL
                     LRLVLGREEPDAGEVMRANAVRIATVEQALEGDLEESLHDYVAGAFADLHRIEAEMRR
                     LEHAMADGQDSAALHDRYDALSHRFENEGGYDMVAEVDKALSGLGFDKVDFGRRLAEL
                     SGGQKNRAMLARAILSSPDVLLLDEPTNHLDFQAVEFLEEYLARSRRAYLVVTHDRRF
                     LDRVADHIIDLESGRLSSYSGGYSAYRRQKAERALTATRAFEKQQDFIEKEKEYIRRN
                     IAGVNSRQAKGRRTKLARVERLERPVEDGTSVGFRFDAARIGGRTFLRAKHLDAGYAA
                     GHPIVRGVDFELLRGERMALLGENGTGKTTLVKTLAGRLAPLGGTVTSGHDVSIGYYD
                     QELSDLDPKRRVIDAVWDEHPDETEEAMRSYLARFAF"
BASE COUNT          281 a          554 c          581 g          295 t
ORIGIN      
        1 atctggccgt cggagacatc aatcgagacg gggatgaaga cctggcggta gcgaatcagt
       61 tctgttgcgg aaccgatgtc tctggccctg ccttgtcgat tctgtcgggc aacggaatcg
      121 gtggattcgc ttctccttcg aacttcgcag tcgggacgcc ggttttcgtt ccgggaccct
      181 ttgcagtggt gctcgccgat ttcaaccgag acgggcagct tgatgtcgcg ctcggcgact
      241 cgggaagccg tatctggatc tacctcgcat gtgcggctgt tcgtgccgac atcccgacgc
      301 tcggtcctct cggcctgatc gtgatgggtc tgctcgtcgc gctcgtggcg ctgagccgtt
      361 tgaacgcccg aggctgattt cgcgacccat tcggctcccg cggccgggcg gctagaatct
      421 cacggtgctc tatcggttcg atcgcgttcg taagtcctac ggctccaagg aggtgctgcg
      481 cgaagtcacc tggcagcaca accccggtga gaaggtcggt ctggtcggcc ggaacggggc
      541 gggcaagacg acgcttctgc gcctggtcct cggccgcgag gagccggacg cgggcgaggt
      601 catgcgggcg aacgcggtcc gcatcgcgac ggttgagcag gcgctcgaag gagacctcga
      661 agaatcgctc cacgactatg tcgccggagc tttcgcggac ctccaccgga tcgaggcgga
      721 gatgcgccgg ctcgaacacg cgatggccga tggacaggac tccgctgccc tccacgaccg
      781 gtacgacgcc ctttcgcacc ggttcgagaa cgagggcgga tacgacatgg tcgccgaagt
      841 ggacaaggcg ctctcgggcc tcggcttcga caaggtggac ttcggccgcc gtctcgcgga
      901 gctctccggc ggccagaaaa accgggccat gctcgcccgc gcgattttgt cgtcgccgga
      961 cgtcctcctg ctcgacgagc cgaccaacca cctcgacttc caggcggtcg agttcctcga
     1021 ggaatacctg gcgcgctccc gccgcgcata cctcgtcgtc acgcacgacc ggcggtttct
     1081 cgaccgcgtg gccgatcaca tcatcgatct cgagagcggc cggctctcct cgtactcggg
     1141 cggctacagc gcgtaccggc ggcagaaggc ggagcgcgcc ctgacggcga cgcgggcctt
     1201 cgagaagcag caggatttca tcgagaagga gaaggagtac atccgccgca acatcgccgg
     1261 ggtcaactcg cgccaggcga agggtcggcg gaccaagctc gcgcgcgtcg agcggctcga
     1321 gcgcccggtc gaggacggca cgtcagtcgg gttccgcttc gacgcggcgc ggatcggcgg
     1381 gcgcaccttc ctccgtgcga agcacctcga cgccggatat gcggcagggc atccgatcgt
     1441 ccggggagtg gacttcgagc tcctgcgtgg cgagcggatg gcgcttctcg gcgagaacgg
     1501 gaccgggaag acgacgctcg tcaagacgct tgccgggcgg ctcgcgcccc tcggaggaac
     1561 agtgacatcc gggcacgacg tctcgatcgg ctattacgac caggagctct ccgacctcga
     1621 tccgaagcgg cgcgtcatcg acgcggtgtg ggacgagcac cccgatgaga cggaggaggc
     1681 gatgcgctcg tatctcgcgc gtttcgcctt c
//
LOCUS       DASWAV010000409         2143 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U2665
            k141_503446, whole genome shotgun sequence.
ACCESSION   DASWAV010000409 DASWAV010000000
VERSION     DASWAV010000409.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960316
            Sequence Read Archive: SRR10260007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2143)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2143)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:47:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,929
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2143
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_503446"
                     /isolate="SMAG_U2665"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.36444 N 132.31889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..927
                     /locus_tag="VF958_08465"
     CDS             <1..927
                     /locus_tag="VF958_08465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9149177.1"
                     /translation="PPVPSPQSVVLQLDRRGVEKPLPLPAGSYDDPRLSPDGKTLALG
                     KCDGESCKLVLYDLARNLLTPFTTEPGRFFSAVWSPDGRRLAYSGFAVGAPTLWVKNA
                     DGSGAAQRLTNAPTEKREAAEFPNSWSADGKTIAYAAVYGLTGGRAARDIWLASADEK
                     GKSRPWLETPYAESAAAFSPDGRWMAYVSDESGREEVYVRPLSGAGGRIKVSSEGGFE
                     PVWTRGGRELFYRNGNQFLSVDIQSEPALAAGAPHVLFSGDFTPGGREDAPFEYAVSS
                     DGNMIYATRVVAQPEPERRLAIVTNWIPAPGR"
     gene            complement(941..>2143)
                     /locus_tag="VF958_08470"
     CDS             complement(941..>2143)
                     /locus_tag="VF958_08470"
                     /EC_number="2.3.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015350009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046912 - acyltransferase activity, acyl
                     groups converted into alkyl on transfer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate synthase"
                     /protein_id="HEX9149178.1"
                     /translation="LRQIKVDENDFGLLSYDPAFLNTASCKSRITFIDGEKGILRYRG
                     YPIEQLAEKCTYLEVAYLLLNGELPTPSQHSNWVREITFHTMLHESLKKFMDGFNYDA
                     HPMGMLIATVAAMSTFYPEARNIHDPASRRQQTYRLIAKMPTLAAFAYRHSLGQPYVY
                     PDNELSYTENFLNMLFKFPADSYRPHPTLVKALDVLFILHADHEQNCGTHAMRGIGSS
                     QTDPYSAVAGAASALYGPLHGGANEEVLKMLREIGSKSGVPAFIEEVKKGKGEKRLMG
                     FGHRVYKNYDPRARIIKQIADEVFDVTGRNPLLDVALELERIALQDDYFVKRKLYPNV
                     DFYSGLIYQAMGIPTAAFTVMFAIPRTAGWIAQWEEMLLDPEQKIARPRQLYMGQPER
                     NFVAMEDR"
BASE COUNT          367 a          685 c          699 g          392 t
ORIGIN      
        1 ccgcccgtcc ccagcccgca gtcggtcgtg ctgcagctcg accggagggg agtcgaaaag
       61 ccgctgccgc tgcccgccgg ttcctatgac gatccgcgtc tgtcgccgga cgggaagacc
      121 ctggccctcg gaaaatgcga cggcgagtcc tgcaagctcg tcctttacga cctcgctcgt
      181 aacctgctca ctcccttcac caccgagccg ggccgtttct tcagcgccgt ctggtccccg
      241 gacggccgcc ggctcgccta ctcgggtttc gcggtcggtg cacccactct gtgggtgaag
      301 aacgcggacg gaagcggcgc cgcgcagcgc ctcacgaacg ccccgacgga gaagcgggag
      361 gcggccgagt tcccgaactc ctggtcggcc gatgggaaaa cgatcgccta cgccgccgtc
      421 tacgggctta ccggaggacg agccgctcgc gacatctggc tcgcttccgc cgacgaaaag
      481 ggaaagtccc gcccgtggct cgaaactccg tacgcggagt ccgccgcggc gttctcgccg
      541 gatgggcgat ggatggcgta cgtctcggac gaatccggcc gggaggaggt ttacgtgcgg
      601 cctctctcgg gcgccggcgg ccgaatcaag gtctcgagcg agggcgggtt cgaaccggtg
      661 tggacgcgcg gcgggcgcga gctcttctac cggaacggca atcagttcct gtccgtcgac
      721 atccaatcgg agcccgcgct cgccgccggt gctccccacg tcctcttctc cggagacttc
      781 acgcccggcg ggagagagga cgcgccattc gagtatgccg tctcgagcga cggcaacatg
      841 atctacgcga cgcgggtcgt cgcccagccc gaacccgagc gccggctcgc catcgtgacg
      901 aactggatac cggcgccggg tcgctaggct cgggaatctc tcagcgatct tccatggcca
      961 cgaagttgcg ctccggctgg cccatgtaga gctgccgcgg gcgcgcgatc ttctgctcgg
     1021 gatcgaggag catttcctcc cactgcgcga tccaacccgc cgtgcgcgga atcgcgaaca
     1081 tcacggtgaa ggccgcagtt ggaatcccca tggcctgata gatcagcccg gaatagaagt
     1141 cgacgttcgg gtacagcttg cgcttcacga agtagtcatc ctgaagggcg atccgctcga
     1201 gctccagggc gacgtcgaga agcgggttcc gtcccgtcac gtcgaagact tcgtcggcga
     1261 tctgcttgat gatgcgggcc cgcgggtcgt agttcttgta gacccgatgt ccgaatccca
     1321 tcaggcgctt ctcgcccttc cccttcttga cctcttcgat gaacgccggg accccggact
     1381 tcgagccgat ctcgcggagc atcttcagga cttcttcgtt ggctccgccg tgcagaggcc
     1441 catagagagc ggacgccgct cccgcgaccg cggagtaggg gtcggtctgc gacgagccga
     1501 tgccgcgcat cgcgtgcgtt ccgcagttct gctcgtggtc cgcgtggaga atgaacaaca
     1561 cgtcgagcgc cttgacgaga gtcggatgcg gacggtagga atcggccggg aatttgaaca
     1621 acatgttcag gaagttctcg gtgtacgaga gctcgttgtc cgggtacacg tagggctgtc
     1681 cgaggctgtg gcggtacgcg aacgctgcga gggtgggcat cttcgcaatc aaccgatacg
     1741 tctgctggcg ccgggaagcc gggtcgtgga tgttccgggc ctccgggtaa aacgtggaca
     1801 tggcggcaac cgtcgcgatc aacatcccca tcggatgcgc gtcgtagttg aaaccgtcca
     1861 tgaacttctt caaggattcg tggagcatcg tgtggaaggt gatctcccgc acccagttcg
     1921 agtgctggct cggggtcgga agctcgccgt tgagaagcag ataggcgacc tcgagatacg
     1981 tgcatttctc ggcgagctgc tcgatcggat agccccggta acgcaggatg cccttctctc
     2041 cgtcgatgaa ggtgatccgg ctcttgcagg acgccgtgtt caggaaggcc ggatcgtagg
     2101 agagcaggcc gaagtcattc tcgtccacct tgatctgtcg aag
//
LOCUS       DASWAV010000410         3827 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U2665
            k141_579839, whole genome shotgun sequence.
ACCESSION   DASWAV010000410 DASWAV010000000
VERSION     DASWAV010000410.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960316
            Sequence Read Archive: SRR10260007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 3827)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3827)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:47:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,929
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3827
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_579839"
                     /isolate="SMAG_U2665"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.36444 N 132.31889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            162..2351
                     /locus_tag="VF958_08475"
     CDS             162..2351
                     /locus_tag="VF958_08475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012411436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HEX9149179.1"
                     /translation="MSHRLRSLARSLAFSGFAVALLVFPALSGLAQQPAPSSAPSKIP
                     ARYEYPSAPRGEVVEDYHGTKVADPYRSLENPGDPTTVAWVDSENRLTRSLLDRPERA
                     RIKARLTELFDYPKIGIPEKKGGRYFFFKNAGLQNQSVYYVQEGPNGEPHVLIDPNTM
                     SPDGTAALTNTSPSKDGRLLGYAVSKSGSDRQEIFVRDVATGKDLPDKLVWVKFSGIS
                     WTPDGKGFFYTRYPEPGTVPSGDEHYFPKFYYHRLGEAQERDSMFFEKPTEKEVGVGG
                     FVSWDGRWLVILPGKGASDKTEVWALDLKKPGAKPFAVFTGFDNAYQVAEVMNGKLYA
                     RTDRGAPLGRIISVDLTTLSSDNKDAPFKEIVSQGTDTIQAAYILNAKLVVRRLRSAS
                     TALEVRDLTGKFEREIRLPEIGSVGAISGEVDDPEMFLSFSSFTMPPTNYRYDLRRRQ
                     LVLFSQPHVKVDPSLYETEQVWYPSKDGTKVSMFLVHRKGLKKDGNRPVYLSAYGGFN
                     ISSTPGFSATRYLFLEKDGVVAIPNLRGGGEYGEEWHKAGMREKKQNVFDDFISAAEW
                     LIANGWTKASRLAIQGGSNGGLLVSAVEMQRPDLYGAVICQVPVADMLRYQKFTVGRY
                     WISEYGSSEQAADFPFLYKYSPYHNVREGVSYPATLVTTADTDDRVAPGHGKKLAARL
                     QAAQAGDEPILLRVETKAGHGAGKPTTKILDEQADIYTFLFWRLGMT"
     gene            2459..3271
                     /locus_tag="VF958_08480"
     CDS             2459..3271
                     /locus_tag="VF958_08480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9149180.1"
                     /translation="MRHRTQSIVPLLALFAAAAPLSGATRQAFSLTVVVDGCEAREYD
                     HLGRVYVEALRGRDFSLRISNPTPERIAVALSVDGRNVIDARRTSTLEAAKWVLSPGE
                     TIDVPGWQVSGQTSRKFFFTETSASYAKWLGDTRNVGTIEAVFFREKRRRPVALQGTV
                     GGGNAPEPLSAAAPPDVPAAEARSEAAAPQRKSEADRFAATGIGDRTDFAVQWIEFEE
                     DPAPAARIALRYEFRRELVRLGVLPRSDDALAARERARGFEPRYAPDPDRQR"
     gene            3279..>3827
                     /locus_tag="VF958_08485"
     CDS             3279..>3827
                     /locus_tag="VF958_08485"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEX9149181.1"
                     /translation="MSDGRPEVENTIAAFPGRRALDTVLPSASDEDLIARARAGDEPS
                     FEEFVRRHMTAVHRWMVHAVGEADADDLTQDVFLRAYRGLPRFRDQAPPRAWLAAIAN
                     NAIKNRYRSRSRFRRIFSPRPEPDSLADRESDAASPEENARAGESRHSVLGALSKLPI
                     EFRLPVVLRDLEEWSYEEIATSL"
BASE COUNT          674 a         1286 c         1247 g          620 t
ORIGIN      
        1 ggcgaacgac acgatcaccg tccaggtcgt ctcgggccgg gcaatcgtgt acggagcgac
       61 gatcgacaac cggacgaacg acccgagcat gcagctggca agacgggcgc cctgaggtct
      121 cccgccggtg ttaaatagag accccacaac aggaggtgtc gatgtctcac cggctccgtt
      181 cgctcgcgcg atcgctcgcc ttctccggct tcgcggtcgc ccttctcgtt tttccggcgc
      241 tgtccgggct cgcgcagcag cccgcgccct cgtccgctcc gtcgaagatc ccggctcggt
      301 acgaataccc ctccgctccc aggggagagg tcgtcgagga ctaccacgga acgaaggtgg
      361 ccgacccgta ccgctcgctc gagaacccgg gtgacccgac gacggtcgcg tgggtggact
      421 ccgagaaccg gctcacgcgg agcctgctcg accggccgga gcgcgcaagg atcaaggcgc
      481 gcctgaccga gctcttcgac tatccgaaga tcggcatccc cgagaagaag ggcggccgct
      541 acttcttctt caagaacgcg ggcctccaga accaatccgt ctactacgtg caggagggcc
      601 cgaacgggga gccgcacgtg ctcatcgacc ccaatacgat gtctcccgac ggcaccgccg
      661 cccttaccaa cacctcccct tccaaggacg gccggctcct cggttacgcg gtttccaaga
      721 gcggcagcga tcgacaggag atcttcgtcc gcgacgtcgc gacggggaag gacctccccg
      781 acaagctcgt ctgggtgaag ttctccggga tctcgtggac acccgacggg aagggcttct
      841 tctacacgcg ctatcccgag ccggggaccg tgccatcggg cgacgagcac tacttcccga
      901 agttctacta ccaccgactc ggcgaggcgc aggaacggga ctcgatgttc ttcgagaagc
      961 cgaccgagaa ggaggtcggc gtcggggggt tcgtctcgtg ggacgggcgc tggctcgtca
     1021 tcctgccggg aaaaggcgcg agcgacaaga cggaggtgtg ggcgctcgac ctcaagaagc
     1081 cgggcgccaa acccttcgcg gtcttcaccg gcttcgacaa cgcctaccag gtcgccgaag
     1141 tcatgaacgg caagctctac gcacgcacgg accgcggcgc tcccctcggc cggatcatct
     1201 ccgtcgacct gaccacgctc tcgtccgaca acaaggacgc gccgttcaag gaaatcgtgt
     1261 cccaggggac ggacacgatt caggccgcct acatcctgaa cgcgaagctc gtcgttcgaa
     1321 ggctgaggag tgcttcgacg gcgctcgagg tgcgcgactt gaccgggaag ttcgagaggg
     1381 aaatccggct gccggaaatc ggctccgtgg gcgcgatctc cggcgaggtg gacgacccgg
     1441 agatgttcct ttccttttcc tccttcacga tgccgccgac gaattaccgc tacgacctcc
     1501 gcaggcgtca gctcgttctc ttctcccagc cgcacgtgaa ggtcgatccc tctctctatg
     1561 agaccgagca ggtctggtat ccgtcgaagg acggcaccaa ggtctcgatg ttcctggttc
     1621 accgaaaggg cttgaagaag gacggcaacc gccccgtgta cctttcggca tacggcggtt
     1681 tcaacatcag ctcgacgccc ggcttctccg cgacgcgcta cctcttcctc gagaaggacg
     1741 gcgtggtcgc catccccaac ctccgcgggg gcggcgagta cggcgaggag tggcacaagg
     1801 ccggcatgcg agagaagaaa cagaacgtct tcgacgactt catctccgcc gccgagtggt
     1861 tgatcgcaaa cggatggacg aaagcctcgc gtcttgcgat ccagggggga tcgaacgggg
     1921 ggctgctcgt atccgcagtc gagatgcaga ggccggatct ctacggggcc gtgatctgcc
     1981 aggtgccggt cgccgacatg cttcgatatc agaagttcac ggtgggccgt tactggatct
     2041 ccgagtacgg tagctcggag caggccgcgg acttcccatt cctctacaag tactcgccgt
     2101 accacaacgt tcgcgagggc gtgtcgtatc cggccacgct cgtgacgacg gccgacaccg
     2161 atgaccgcgt cgcacccggt cacggaaaga aactggcggc ccgtctgcag gcggctcagg
     2221 ccggcgacga acccattctc ctccgcgtcg aaacgaaggc cggacacggt gcgggcaagc
     2281 ccacgacgaa aatcctcgac gagcaggcgg acatttacac cttccttttc tggcggctcg
     2341 gcatgaccta gcttcgaagg tcggagatcg aaaatcggaa gtcctcaaaa gggccttccc
     2401 gccctgccgt tggggaacct tgaacggggg gcggtgtcct tctcgacagg aggccgccat
     2461 gcgacaccga acgcaatcga tcgtccccct tctcgccctc ttcgccgccg cagctcccct
     2521 ttccggcgcc acgcgacagg cgttctcgct caccgtcgtc gtggacggat gcgaggcgcg
     2581 ggagtacgac catctcggcc gggtctacgt tgaggctctc cgcggccggg atttctcgct
     2641 gcgaatctcg aacccgaccc ccgagcggat cgcggtcgcg ctctccgtcg acgggcgcaa
     2701 cgtcatcgac gcgaggcgga cgtcgacgct cgaagcggcg aagtgggtgc tctcccctgg
     2761 cgagacgatc gatgttccgg gctggcaggt ctccgggcag acgtcccgga agttcttctt
     2821 cacggagacg tcggcctcat acgcgaagtg gctcggcgat acgagaaacg tcggcacgat
     2881 tgaagcggtc ttcttccgcg agaagcgtcg gcggccggtc gcgttgcagg gcacggtcgg
     2941 cgggggaaac gccccggagc cgctctccgc cgcggctccg cccgacgtcc cggcagcaga
     3001 ggcccgctcg gaggcggccg ccccgcagcg taagtcggaa gccgaccgtt tcgcggcgac
     3061 gggaatcggc gacaggaccg acttcgccgt ccagtggatc gagttcgagg aagacccggc
     3121 tcccgcggcg cgaatcgccc tcaggtatga gttccgtcgg gagctcgtgc ggctgggcgt
     3181 actgccgcgc tccgacgacg cgctcgcggc ccgggaacgc gcccgcggct tcgagccgcg
     3241 ctacgcgccc gacccggatc ggcagcggta agcacgcggt gtcggacggc cgcccggagg
     3301 tggagaatac gatcgcggcg ttcccggggc gccgtgcgtt ggacaccgtg ctcccctccg
     3361 cttccgacga ggacttgatc gcccgcgcga gagcgggcga tgagccgtcc ttcgaggagt
     3421 tcgttcgccg gcacatgact gcggtgcatc ggtggatggt ccacgcggtc ggagaagccg
     3481 acgcggacga tctgacgcag gacgtcttcc tgcgcgccta ccgcggcctg ccccggtttc
     3541 gagaccaggc gccgccccgt gcgtggctgg cggcgatcgc caacaatgcc atcaagaatc
     3601 gttaccgctc gcggagccgg ttccgcagga tcttttcgcc gcggccggag ccggactcgc
     3661 tcgcggaccg ggaatccgac gccgcgagcc cggaagagaa cgcgagagcg ggagagtcgc
     3721 ggcactcggt cctcggagcg ctctcgaagc taccgatcga gttccggctg ccggtcgtcc
     3781 tgcgtgacct cgaggagtgg agttacgagg agatcgcgac gtcgctc
//
LOCUS       DASWAV010000411         1874 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U2665
            k141_662328, whole genome shotgun sequence.
ACCESSION   DASWAV010000411 DASWAV010000000
VERSION     DASWAV010000411.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960316
            Sequence Read Archive: SRR10260007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 1874)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1874)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:47:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,929
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1874
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_662328"
                     /isolate="SMAG_U2665"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.36444 N 132.31889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..811)
                     /locus_tag="VF958_08490"
     CDS             complement(<1..811)
                     /locus_tag="VF958_08490"
                     /inference="COORDINATES: protein motif:HMM:NF013328.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-formamidopyrimidine glycosylase family
                     protein"
                     /protein_id="HEX9149182.1"
                     /translation="MPEGNTIHRVAREQTKDFAGKVVRARSPQGRFANESRRLDRRLF
                     VRAEAYGKHLFQFWEGRRVVHVHLGMAGDFYRFEGSAPPPRPTVRLRLSTPEVTVDLI
                     GPPTCELVTEEERRAILARLGPDPLRRDADPQVVRTALERRPDRPIGDALLDQRVIAG
                     VGNIYRNEALFLTGIHPLRAAGRISEDEWLAVWKTLSSLMRRGVREGTVTADPTEPPH
                     PRAASVRAPDDDDFYVYGRTFCRRCGSRLRELPLSGRRMFACPRCQGRRRRG"
     gene            871..>1874
                     /locus_tag="VF958_08495"
     CDS             871..>1874
                     /locus_tag="VF958_08495"
                     /inference="COORDINATES: protein motif:HMM:NF017745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF885 family protein"
                     /protein_id="HEX9149183.1"
                     /translation="MDLTLVCLLATALGSASVMARPSTRYEDLLSLFRDWRSFQKPRL
                     VDGVPDYTPAAMAAQHQELASWQQRLAAIDPSGWPVGRQVDWHVVRAEMNGLDFDHRV
                     AKPWANNPAFYVTVFTEESDQPAREGPFAYGAVELWSHTFPLTAESAARIDAGIQAIP
                     GLLGQAKKNLVGSGRDLWISGAKSIRQQSADLAELASRLTETAGSLKASVEAAKKATD
                     DFAAWLDSQAASKTGPSGVGVENYDWYLKNVQLAPYTWRDEVTIMERELARAHALLAM
                     EELRNARLPEQVPVASAEEYDRRFGAAVTEYMAFLREHDVLTVTGDMDAALRARIGSF
                     "
BASE COUNT          310 a          626 c          660 g          278 t
ORIGIN      
        1 aaccgcggcg cctccggccc tggcagcgcg gacacgcgaa catcctccgg ccggagagcg
       61 gcagctcgcg caaccgcgat ccgcacctcc ggcaaaaggt ccggccgtag acgtagaagt
      121 cgtcgtcgtc cggcgcgcgg acggacgccg cgcgtggatg cggcggctcg gtgggatctg
      181 ccgtgacggt cccttcccgg accccgcgcc gcatgagaga ggagagcgtc ttccagaccg
      241 cgagccactc gtcctcggag attcttccgg cggcgcgcag cggatggatg ccggtcagga
      301 acagcgcctc gttgcggtag atgttgccga cgccggcgat gacacgctgg tcgaggagcg
      361 catcgccaat cggccggtcc ggccgtcttt cgagggccgt ccggacgacc tgcgggtcgg
      421 cgtcccggcg gaggggatcg gggccgaggc gcgccaggat cgcccggcgt tcctcctcgg
      481 tgacgagctc gcaggtcggc gggccgatga gatcgacggt gacctccggc gtcgacaggc
      541 gcagccgcac ggtcgggcgc ggagggggag cgctgccctc gaaccgatag aagtcgccgg
      601 ccatcccgag gtggacgtgg acgacgcgcc ggccttccca gaattgaaag aggtgcttcc
      661 cgtacgcctc cgcgcggacg aagagccgcc tgtcgagccg ccgcgactcg ttcgcgaaac
      721 gaccctgcgg agaccgtgcg cgaacgactt ttccggcgaa gtctttcgtc tgctcgcggg
      781 cgacgcgatg aatcgtgttg ccttcgggca tcgggcacga aggttaaccc tctccttcct
      841 gtgttcgaat gttccctcgg aggttggacg atggacctga cgctcgtgtg tcttctggcg
      901 accgctctcg ggagcgcttc ggtcatggct cgccccagca cccgctacga ggacctcctc
      961 tccctctttc gagactggag gtccttccag aagcccaggc tcgtggacgg cgtgcccgac
     1021 tacacgcccg ccgccatggc cgcgcagcac caggagctcg cgtcctggca gcagcgactg
     1081 gccgcgatcg acccgagcgg ctggccggtc gggaggcagg tggactggca cgtcgtgcgg
     1141 gccgaaatga acggtctcga cttcgaccac cgggtcgcca agccgtgggc caacaacccc
     1201 gccttctacg tgacggtctt caccgaggag agcgatcagc ccgcgcgcga ggggccgttc
     1261 gcgtatggag cagtggagct ctggagccac acgtttccgc tcaccgccga gagcgccgcg
     1321 cgaatcgacg cgggaatcca ggcgatccct ggccttctcg gtcaggcgaa gaagaacctc
     1381 gtcgggagcg ggcgcgactt gtggatctcc ggcgcgaaga gcatccggca gcagagcgcg
     1441 gacctggcgg agctcgcctc aagactcacg gaaaccgccg ggagcctgaa agcgagcgtc
     1501 gaggccgcga agaaagcgac ggacgatttt gccgcatggc tcgactcgca ggccgcgtcg
     1561 aagaccgggc cgtccggcgt gggcgtcgag aactacgact ggtacctgaa gaacgtccag
     1621 cttgcgccgt acacgtggcg ggacgaggtc accatcatgg agcgcgagct cgcgcgcgcc
     1681 cacgcgctgc tcgccatgga ggagctgcgc aacgcaagac ttccggaaca ggtgccggtc
     1741 gcgagcgccg aggagtacga ccgacgtttc ggtgcggcgg tgaccgagta catggcattt
     1801 ctccgcgagc acgatgtcct caccgtgacc ggggacatgg atgccgcgct gcgcgcgagg
     1861 atcggcagct tcca
//
LOCUS       DASWAV010000412         1697 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U2665
            k141_702793, whole genome shotgun sequence.
ACCESSION   DASWAV010000412 DASWAV010000000
VERSION     DASWAV010000412.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960316
            Sequence Read Archive: SRR10260007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 1697)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1697)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:47:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,929
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1697
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_702793"
                     /isolate="SMAG_U2665"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.36444 N 132.31889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..360
                     /locus_tag="VF958_08500"
     CDS             <1..360
                     /locus_tag="VF958_08500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018049197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HslU--HslV peptidase ATPase subunit"
                     /protein_id="HEX9149184.1"
                     /translation="RFPIRVELDSLTEEDFVRILTEPENALLKQYRALLSAEGVDLAF
                     TDDAVREIARDAERVNRALENIGARRLHTILERLLEDISFAGPENSGRSISIGVDDVR
                     TALADLVRDEDLSRYVL"
     gene            380..1534
                     /locus_tag="VF958_08505"
     CDS             380..1534
                     /locus_tag="VF958_08505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9149185.1"
                     /translation="MLAAALLVPVTAGCGKKGDPMPPLPRGPNAVSDLAVEQEGAEAV
                     LTFSYPDRLMNGEPLRDVSSIEIYRVPDPSPAIAPAKPPAAHGPAHGPAPAGDEAPGS
                     AARREAANVRLAEEAFYKEAKRVDALPVTAIAERTRGATVVYRDPLYPLLSAGKPPAA
                     LAYAVVTVRRGGERSPLSNIVKFSPDVPPGPPAISSVTVEEGRICIEWIAPPTDLLGR
                     PAAVGGYRVYRRTLPDEEYGEPLNPEPVVGTNFVDTTAPYGGSHVYTIRATLAGKPKV
                     EGLPAMEAGVAYRDVFPPAAPSRIDALPEANAVRLVWEPVPVADLAGYVVFRAEGDGA
                     PVRVTEKPITDTFFTDTAVQPRRRYRYTVIAVDTAGNSSASSPEAVAETL"
BASE COUNT          246 a          614 c          584 g          253 t
ORIGIN      
        1 cggttcccga tccgcgtgga gctcgactcg ctgaccgagg aggatttcgt ccgcatcctg
       61 accgagccgg agaacgcact cctgaagcag taccgggcgc tcctctcggc ggagggcgtg
      121 gatctcgcgt tcaccgacga cgcggtccgc gaaatcgcgc gcgacgccga gcgcgtcaac
      181 cgggcgctcg agaacatcgg cgcgcgccgg ctccacacga tcctcgagcg gctcctcgag
      241 gacatctcgt tcgccggccc cgagaattcc ggccgctcga tctcgatcgg cgtggacgac
      301 gtccgcacgg cgctcgccga cctcgtccgc gacgaggacc tgtcccgtta cgtcctgtag
      361 aagttcgttc cggcgagcgg tgctcgccgc cgccctgctc gtcccggtca ccgccggttg
      421 cggcaagaag ggcgacccga tgccgccgct tccccggggg ccgaacgccg tctccgacct
      481 cgcggtcgag caggagggcg cggaggcggt cctcacgttc tcctacccgg accggctgat
      541 gaacggcgag ccgctgcgcg acgtctcctc gatcgagatc taccgggtcc cggatccctc
      601 gccggcgatc gcgcccgcga aacccccggc cgcgcacggc cccgcgcatg gccctgcccc
      661 tgccggcgac gaagcgcccg gatccgcggc gcgtcgagaa gccgcgaacg tccggctcgc
      721 cgaagaggcg ttctacaaag aggcgaaacg agtcgatgcc ctgccggtca cagccatcgc
      781 ggagcgcacg cgcggagcga cggtcgtgta tcgcgatccg ctctatccgc tcctctccgc
      841 gggcaagccg ccggcagcgc tcgcgtacgc cgtcgtgacg gtgcggcgcg ggggagagcg
      901 cagcccgctt tcgaacatcg tgaagttctc ccccgacgtg ccgccgggcc ctccggccat
      961 ctcctccgtg acggtggagg aggggcggat ctgcatcgag tggatcgctc cgccgaccga
     1021 cctcctcggg cgcccggccg cggtcggagg gtaccgggtg taccgccgca cgctccccga
     1081 cgaggagtac ggcgagccgt tgaaccccga gcccgtcgtc ggcacgaact tcgtcgacac
     1141 gaccgcgcct tacggcggct cccacgtgta cacgatccgc gcgacgctgg ccggcaagcc
     1201 gaaggtcgaa gggcttccgg cgatggaggc aggcgtcgcc taccgcgacg tctttccgcc
     1261 agccgcgccg tcgcgcatcg acgcgctgcc cgaggccaac gccgtgcgtc tcgtgtggga
     1321 gccggtcccg gtcgccgatc tcgcgggata cgtcgtcttc cgcgccgaag gtgacggcgc
     1381 gccggtgcgc gtgacggaga agccgatcac cgatacgttc ttcacggata cggcggtaca
     1441 gccgcggcgg cgataccggt atacggtgat cgccgtcgac accgcgggga actcgagcgc
     1501 gtcgtcgccg gaggcggtcg cggagacgtt gtgagtcagt tcgtcttcgc cgggcggatc
     1561 cgttccagct cctgcgacca gcgtaggacg acgttgatca ccgacgggtt cgtgacgtcc
     1621 tgatcccggt cgcggatcat gaggaagcgc ttcccatccg gagtgacgtc gtagttcgac
     1681 gaggtcggtc ccggggg
//
LOCUS       DASWAV010000413         4603 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U2665
            k141_662349, whole genome shotgun sequence.
ACCESSION   DASWAV010000413 DASWAV010000000
VERSION     DASWAV010000413.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960316
            Sequence Read Archive: SRR10260007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 4603)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4603)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:47:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,929
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4603
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_662349"
                     /isolate="SMAG_U2665"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.36444 N 132.31889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(18..1844)
                     /gene="glmS"
                     /locus_tag="VF958_08510"
     CDS             complement(18..1844)
                     /gene="glmS"
                     /locus_tag="VF958_08510"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014551283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004360 - glutamine-fructose-6-phosphate
                     transaminase (isomerizing) activity [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HEX9149186.1"
                     /translation="MCGIVGYVGAKEAAPLLLDGLKRLEYRGYDSAGIATLCNGRFTV
                     HRSAGKLSTLLEKMAGTKAPGTVGIAHTRWATHGRPTEENAHPHTDCTGRIVAVHNGI
                     FENFAERKAELASRGHEFSSETDTEVFAHEVEEAFRGDLFAAVQSATKKLTGAYAVLV
                     SSSAEPGVLVAARQGPPIALGLGENENWVASDPVALIPWTRDVVFLEDGDVARVDAKS
                     VRIVGSDGRPIDRLAHRILWDVVSAEKGGYRHFMAKEIHEQPTAVAETFGGRVALDTG
                     ELTLDSALLERDRVANFDRVLLLACGTSWHAALIGKFLIEGMARLPVEVDYGSEFRYR
                     SPVVDERTLTVGISQSGETADTVAALAEARRAGSPIAAIVNVPGSQIARMAESVLTTH
                     AGPEIGVASTKAFTTQLAVLFLIAARLRESRGLGHGLSAEERTALARLPAALQRTLAL
                     EPKIEHQARNFHDAKNFLFLGRGLHYPIALEGALKLKEISYIHAEGYPAGEMKHGPIA
                     LVDENLPVVVLAPHDAWRDKTISNLREVKSRDGISLVVATRDDAEVSSLADWSLVIPE
                     TIPELQPIVTVVPLQLLAYHMAVRRGCDVDQPRNLAKSVTVE"
     gene            complement(1845..3293)
                     /gene="glmU"
                     /locus_tag="VF958_08515"
     CDS             complement(1845..3293)
                     /gene="glmU"
                     /locus_tag="VF958_08515"
                     /EC_number="2.3.1.157"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: protein motif:HMM:TIGR01173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003977 - UDP-N-acetylglucosamine
                     diphosphorylase activity [Evidence IEA];
                     GO_function: GO:0019134 - glucosamine-1-phosphate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HEX9149187.1"
                     /translation="MRGSKTKNPSRKRRPTVRETEPAPRVVVLAAGAGSRMRSALPKV
                     LHRIGGRTLLDAVLDTAEEIAPSRIVAVVGAGRERVEAALGGRAVTIVVQDPPRGTGD
                     AARRALTALSNGSGPVVILSGDAPLLTSVTLAKLIEARRANDLDLVLLTFRPPEAGDL
                     GRVVRDAKGRVRRIVEARNATAREKRLAEANAGVYCFAARALSRALEKLSRNPVNGEY
                     YLTDVVEILTSSGGSVEAVEAEDWREGWGINTRAELVAAEEIERRRSLERALEAGATI
                     VDPATTRIGPKVRLEPDAIVHPFVCLEGMTVVEQGSEVLPFTRVVDSVLGVGAVVGPH
                     CELEGARVGPRARVGPFARLRPGTVLEEDVRVGNFVETKKAILRAGAKASHLTYLGDA
                     EIGAGANIGAGVITCNFDGERKHHTSIGEGAFIGSDSQLVAPVTVGKGAWVGAGSTIT
                     RDVPDGALAITRTPQKNVEGWVARRRARKAGA"
     gene            complement(3498..4166)
                     /locus_tag="VF958_08520"
     CDS             complement(3498..4166)
                     /locus_tag="VF958_08520"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX9149188.1"
                     /translation="MAKRWTEAELRFLRDNASKMSPQALADALSVRIDELEKKLEKIG
                     TPASASAEGPRKAQTLKELSRHTENARKEYDRGVAALQRRKVNEAERHFLELTQKYPE
                     EKELVDRARVYLAVCERQKNSAAASLSEPEDFYYAAVLEKNRGNVDEAIEHLKRAVRK
                     NGGGKVDFLLACCYAQQGEPGSALEHLRRAIDEDQRNRILARHDRDFDPVRDTPEFQD
                     LLAS"
     gene            4301..4591
                     /locus_tag="VF958_08525"
     CDS             4301..4591
                     /locus_tag="VF958_08525"
                     /inference="COORDINATES: protein motif:HMM:NF039822.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5666 domain-containing protein"
                     /protein_id="HEX9149189.1"
                     /translation="MKTSLAAIAAVALVLALGGNAAAAEKTLTATGTVSRLDAAQRTV
                     VVAVTEGPETTFAWTADTKINGTLAPGAKVTIRYTTLPDGQNLAHQISVARS"
BASE COUNT          680 a         1587 c         1564 g          772 t
ORIGIN      
        1 ggacgaggcg atggcggcta ttcgaccgtg accgacttcg ccaggtttct cggctgatcg
       61 acgtcgcagc cgcggcgcac cgccatgtgg taggcgagaa gctgaagcgg cacgaccgtc
      121 acgatcggct ggagctccgg gatcgtctcc gggatcacga gagaccagtc ggcgagcgac
      181 gagacctcgg cgtcatcgcg cgtcgccacg accagcgaga ttccgtcgcg cgacttcacc
      241 tcgcgcagat tcgagatcgt cttgtcgcgc cacgcgtcgt gcggagcgag caccacgacc
      301 gggaggttct cgtccacgag cgcgatcggc ccgtgtttca tctcgcccgc cggatatccc
      361 tccgcgtgga tgtacgagat ctccttcagc ttcagagcgc cttcgagggc gatcgggtag
      421 tggagaccgc ggccgaggaa gaggaagttt ttcgcgtcgt ggaagttgcg cgcctgatgt
      481 tcgatcttcg gctcgagggc gagggtgcgc tgcagcgccg cgggcagccg cgcgagagcg
      541 gtgcgctctt ccgccgagag gccgtggccg agaccgcgcg attcgcggag cctggccgcg
      601 atcaggaaga gcaccgcgag ctgtgtcgtg aacgccttgg tcgacgccac cccgatctcg
      661 ggtcccgcgt gcgtcgtgag aaccgattcc gccattcggg cgatctggga gccgggcacg
      721 ttgacgatcg ccgcgatcgg agatccggcc cggcgcgctt cggcgagagc cgccacggtg
      781 tccgctgtct cgcccgactg cgagatcccg accgtcaagg tccgctcatc gacgaccggg
      841 ctgcggtagc gaaactcgct cccgtagtcc acctcgaccg ggaggcgcgc catgccctcg
      901 atcagaaact ttccgatgag ggcagcgtgc cacgacgtcc cgcatgcgag caggagcacg
      961 cggtcgaaat tcgcgacgcg atcccgctcg agcagagccg aatcgagtgt cagctctccg
     1021 gtatcgagcg ccacccgtcc cccgaacgtc tcggccactg cggtcggctg ctcgtggatc
     1081 tccttcgcca tgaagtggcg gtagcccccc ttctcggcgg agacgacgtc ccagaggatg
     1141 cggtgcgcga gtcggtcgat cggccggccg tcggagccga cgatgcggac gctcttcgcg
     1201 tcgacgcgtg ccacgtcgcc gtcctccagg aagacgacgt cgcgcgtcca ggggatgagc
     1261 gccaccggat ccgaggcgac ccaattctcg ttctccccga gcccgagggc gatgggcggc
     1321 ccctggcgcg ccgccacgag aacgcctggc tcggcgctcg acgagaccag gacggcgtag
     1381 gcgccggtga gcttcttcgt ggcgctctgt accgcggcga acaggtcgcc gcgaaacgct
     1441 tcttcgactt cgtgggcgaa aacctccgtg tctgtctcgc tcgagaactc gtgtccgcgc
     1501 gacgcgagct ccgccttgcg ctcggcgaag ttctcgaaga tgccgttgtg gactgcgacg
     1561 atccggccgg tgcagtcggt gtgcggatgc gcattctcct cggtcgggcg gccgtgtgtc
     1621 gcccagcggg tgtgcgcgat gccgacggtt ccgggcgcct tcgtgccggc catcttctcg
     1681 agaagcgtcg agagcttgcc ggcgctgcgg tggacggtaa agcggccgtt gcagagggtc
     1741 gcgatgcccg ccgagtcgta accgcggtac tcgagacgtt tcaggccatc gaggaggagc
     1801 ggcgccgcct ccttcgcacc cacgtatccg acgatcccgc acatctatgc gcccgccttg
     1861 cgcgcccgcc gccgcgccac ccacccctcc acgttcttct gcggggtgcg ggtgatcgcc
     1921 agcgctccgt cgggaacgtc gcgcgtgatc gtcgagcccg cgccgaccca cgcccctttc
     1981 ccgacggtca ccggcgcgac gagctgtgag tcgcttccga tgaacgcgcc ttcgccgatc
     2041 gacgtgtggt gcttcctctc accgtcgaaa ttgcacgtga tgacgcccgc gccgatgttc
     2101 gctccggcgc cgatctccgc gtcgccgagg tacgtcaggt gggaggcctt tgcgccggcg
     2161 cgcaggatcg ccttcttcgt ctccacgaaa ttgccgacgc gaacgtcctc ttcgaggacc
     2221 gtcccgggac gcagtcgcgc gaagggtccg actcgagccc tcggtccgac ccgcgcccct
     2281 tcgagctcgc agtgagggcc gacgacggcg cccaccccga gcacggagtc gaccacgcga
     2341 gtgaacggca gcacttcgct cccttgctcg acgaccgtca tgccttccag gcagacgaac
     2401 gggtgcacga tcgcgtccgg ctcgagccgg accttcggtc cgattcgggt cgtggccggg
     2461 tcgacgatcg ttgcgcctgc ttcgagcgcg cgctccaggg atcgccggcg ttcgatctcc
     2521 tcggcggcaa cgagctcggc gcgggtgttg atcccccacc cttcgcgcca gtcctccgcc
     2581 tccaccgctt cgaccgagcc tcccgacgag gtcaggattt cgaccacgtc tgtcaggtag
     2641 tactcgccgt tgaccgggtt gcgggacagc ttctcgaggg cccgcgagag cgcccgggcg
     2701 gcaaagcagt agacgccggc gttcgcttcc gcgagccgct tttcacgcgc ggtcgcgttg
     2761 cgcgcttcga cgatgcgccg cacgcgtccc ttcgcgtctc ggacgacgcg cccgagatcg
     2821 cccgcctccg gcggacggaa cgtgagcaac accaggtcga ggtcgttcgc gcgccgcgcc
     2881 tcgatcagct tcgcgagcgt cacggaagtc agaagcggcg cgtcgccgga caggatcacg
     2941 acgggcccgg agccgttcga gagcgccgtg agcgcccgcc gggcggcgtc gccggtgccg
     3001 cgcggcgggt cctggacgac gatcgtgacc gcgcggccgc cgagcgcagc ctcgactctc
     3061 tcccgacccg cgccgaccac cgcgacgatc cgcgagggcg cgatctcctc cgcggtgtcc
     3121 agcacggcgt cgagcaacgt gcgtccgccg atgcggtgaa ggaccttcgg aagcgcggag
     3181 cgcatgcggg atccggcgcc ggccgcgagc acgacgacgc gaggcgcggg ctccgtctcc
     3241 cgcaccgtcg gccgtcgttt ccggctcgga ttcttggtct tggagccgcg catcgaatgc
     3301 ccttcgtcgt ccgccgagga gctgcaagta taggaccgac gaatcacatg cgtcgcagcg
     3361 tgaggagtct ccgcaggaag gagcctcacg ctgcgggagc ggaagcgccg cgagggatca
     3421 ggtgagcggt cgagccgggg ttggcgagca gctcaccggt ggcgtcagcc agatccccag
     3481 caagcgaccg cgacggctca ggacgccagc aggtcctgga actcgggcgt gtcccgcacc
     3541 ggatcgaagt cgcggtcgtg gcgtgcgagg attcgatttc gctggtcttc atcgatcgcc
     3601 cgccggaggt gctcgagagc cgagcccggc tcgccctgct gcgcgtagca gcaggcgagg
     3661 aggaagtcga ccttgccgcc gccgttcttg cggacggcgc gcttcaagtg ctcgatcgcc
     3721 tcgtcgacgt tgccccggtt cttttcgagg actgccgcgt agtagaagtc ctcgggctcc
     3781 gaaagcgagg ccgcggccga attcttctgc cgttcgcaca ccgcgagata gacgcgggcg
     3841 cggtcgacga gctccttctc ctcggggtat ttctgggtga gctccaggaa atgacgttcc
     3901 gcctcgttga ccttccggcg ctggagcgcc gcgactcccc ggtcgtactc cttgcgggcg
     3961 ttctcggtgt ggcgggagag ctccttcaga gtctgggcct tccgcgggcc ctccgccgag
     4021 gcggacgccg gcgtgccgat tttctcgagc ttcttttcca gctcgtcgat cctcacggaa
     4081 agcgcgtcgg ccagggcctg ggggctcatc ttcgacgcgt tgtctctcag gaatcgaagc
     4141 tcggcttctg tccagcgttt tgccattctg ttacctccgg ggggccgcga gaaaaacggc
     4201 gtattgtagg gacggaccct ccgcatgtca acggcgagcg cgccggaatg gctgaactcg
     4261 aaggaacgtt ctcttcgtgg gggtgggtat gaggagaact ttgaagactt cgctcgcggc
     4321 catcgccgcg gtggcgctcg ttctcgcgct cggaggaaac gctgcggccg ccgagaagac
     4381 tctgacggcg accgggacgg tttcccggct cgatgctgcc cagcgcacgg tcgtcgtggc
     4441 cgtgacagag gggcccgaga cgacgttcgc ctggacggcc gacacgaaga tcaacggcac
     4501 tctcgccccc ggggcgaagg tcacgatccg ctacacgacc ctccccgacg gccagaacct
     4561 ggcgcaccag atcagcgtgg cgcgaagcta gctctccctt tcc
//
LOCUS       DASWAV010000414         7412 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U2665
            k141_342035, whole genome shotgun sequence.
ACCESSION   DASWAV010000414 DASWAV010000000
VERSION     DASWAV010000414.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960316
            Sequence Read Archive: SRR10260007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 7412)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7412)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:47:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,929
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7412
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_342035"
                     /isolate="SMAG_U2665"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.36444 N 132.31889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(72..344)
                     /locus_tag="VF958_08530"
     CDS             complement(72..344)
                     /locus_tag="VF958_08530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9149190.1"
                     /translation="MPGVERVAGLHVWAMGTSENALTAHLVMPDGLPSDAFYGDAERE
                     LHDRFGIEHVTLQPVSVSFTAPCAATLQVNGSGSQLGPERFRRAVS"
     gene            complement(478..972)
                     /locus_tag="VF958_08535"
     CDS             complement(478..972)
                     /locus_tag="VF958_08535"
                     /inference="COORDINATES: protein motif:HMM:NF033788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HEX9149191.1"
                     /translation="MIDADLAGPVDEHKALAHPLRLRILAMLGKEPQYVCQITTVLHS
                     AVSTVSTHLAVMKDAGLAIETREGRWVRYALSDRGAQFYSFHREELTAGRDVIADSAI
                     LAIVRTIPREDLCQASLDFRKLRNSELIALARKYRRLGKGRIPEAAPQGCGHQEAAKE
                     NRKD"
     gene            1326..2150
                     /locus_tag="VF958_08540"
     CDS             1326..2150
                     /locus_tag="VF958_08540"
                     /inference="COORDINATES: protein motif:HMM:NF024827.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c3 family protein"
                     /protein_id="HEX9149192.1"
                     /translation="MLIPAQLLYGANPCRDCHEKQVAAIAAGPHAAVAARDARFCATC
                     HGDPKAHLESGDPADIVGGKTLATWRGERQALACASCHEKEFPAWRQAPHTANRLCWS
                     CHASQALHSKGEPPLGPAKAHQTWGLCISCHPGEAAQFRMVYRHPVERGVMDCTDCHD
                     IHGRSVLQTNRVDDHACLRCHEEQKGPFLFEHAGMEQGCSNCHAAHGSPHRGQLLSSG
                     NGACLTCHIQSNFPGIGKTSHDSLLSGGGRCWDCHSEVHGSNTTPDFNPRGRRLRR"
     gene            2147..4084
                     /locus_tag="VF958_08545"
     CDS             2147..4084
                     /locus_tag="VF958_08545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9149193.1"
                     /translation="MKQILAAGSVLLVFLLAPGLTAQTSGSVAVGAQADSTSGNVSAF
                     QEQNGGEESGVFIERLKLLGTGTVPWQIDGRFSTGGSGWLDLSARDGAWSGGLRLTQA
                     RTWSATSFADDALPSGVPVSSLSPPSTTLDPLFGVTDPHGELLHTEAWLTRAIGNASR
                     LTVRAGYRQRDGSRVPNIGAFSFSDVGTPAFFAPGLERIDSSSSWGALEATSLWRGLA
                     LQLDAGASRRDNRTRYDLPAYGTSGLLDLNRWTTGTEADSRWFRLAASRPSERVTVYG
                     GVSWIDVSSTPSGSDLRVTGNGDVVRDGLSLENGSIDATTSAGAAGFTWRMHPAVALT
                     VSADVQSRSSEGSGRILLRSANPVFTTTDTSSDRVGATADLTGRFGATRVHLRGRVTT
                     TNIDRLEDSTPFLQDETRSIDRMEIRGDLSRRLSGDIRARGWVRYADESGSVDLRQLD
                     YGYASSDWDRDDLSGGIELALGSGDRRANLTVNGGKSDVLDTPPLFDPVYDPSLELSD
                     IGGTISTFRAAVTGMRNFARGTAWGEAGWLSTEYEYNASFDQPGFSSMSEKIEGLVAS
                     AGAEVRPRDGTRVIGQVEWVRDRADLDRTLTRASLELAQTIHTRFELFGRWFVGDLDA
                     PHAQQSNYSVNLFAVGVRTNF"
     gene            4098..4541
                     /locus_tag="VF958_08550"
     CDS             4098..4541
                     /locus_tag="VF958_08550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9149194.1"
                     /translation="MSYRNSRFAAIGLALAVLCILGPGGLASPGKANAPTAMGGAAAS
                     TTSLTGIHDPLSRNYDSDCVKCHASILKESSRDPRILAFHQAMIPYTPGYSARKGVQS
                     KNCVTCHRDAIDFRQESGSSLRRTVSIESCVYCHGRTGPGPAYFE"
     gene            4552..5091
                     /locus_tag="VF958_08555"
     CDS             4552..5091
                     /locus_tag="VF958_08555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005435433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HEX9149195.1"
                     /translation="MTRNGFLVDLNYCISCKACEVACKTWNAVPTDRGIRWRRVVDEL
                     LGVVPRTTAYSVSLACNHCENPACVRACPNGALTMRADGLVVHDKTKCVGCRYCESVC
                     PYGAPQYDAVEKVMTKCTGCFDRVDQGLLPACVDACPAEALKFGPLAEIDREGIREIP
                     RFPNPNRTRPAVRFVPKES"
     gene            5093..7348
                     /locus_tag="VF958_08560"
     CDS             5093..7348
                     /locus_tag="VF958_08560"
                     /inference="COORDINATES: protein motif:HMM:NF012602.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HEX9149196.1"
                     /translation="MTKNGFSRRTFLRTSGVAAAGLVAAPAFLESLFAQTFGPLQSLP
                     AGVTQLITACGLCDSVCGLRATMKDGILTFVEGVKEDLHGGGKLCGKGKSAAGFLYDP
                     DRLKYPMKRTNPIKASGSDPGWVRISWDEALGMASETLNQTIAAHGADSVLVISRPAP
                     DIWMRFINAIGTPNRVDHIDECFLSDKIVSGKVLGGKLWGHDLANSKYILLFGWDLVG
                     KAKMAYARQVVKAKKAGAKIVVFNPMWTPTATLADEWHQIRPGSDLALALAMIQVIVS
                     EKLYDAEFVKSYTNFPAYEQQFQDNFAPYTPEWAAELTDIPAHTIRRIAREFAMTRPG
                     IVPTHKKTSAANYANAAPLQHAIAILNCLNGSIDRPGGRFYPRQVELDGVDKIYPPPA
                     YPAKSGVWIDGRDRHPFVKGAGNGMFSTLANGMLKVHPEKIQMAIITKYTTMGFPNPP
                     EIASALAKVPFTVVVDIVPNEIIDLADLVLPQASYLESADIAVREHYARFPQAFVRQP
                     VAPAMFESKGFGWIAIEWGKRSFPDYFKKPDGSWLNMNEILDEKVQRAKLAPSFADFK
                     KAGFYSDEQPFVPKTTFPTPDGKLRLWVQDFADKGFDPLPKWMPKRDLPSAEYPFYLI
                     TVIPGVHIRNTTQNNPFLNEIFGTNFVMIHPETARRLDIEEGDPVRVKSRVGTITLPA
                     RISGGIRPDCVCIPHGFGHRSRLLTKAYGKGERDSDLVPSQSMDEILARRDVGGAGAI
                     MDAVVSLERTA"
BASE COUNT         1385 a         2404 c         2294 g         1329 t
ORIGIN      
        1 gcagtgtagc gcgcgaccgt acgggttgcg ccaggctgcc gctcaaggag aagccatgtc
       61 tcagagcgac atcacgaaac tgctcggcgg aaccgctcgg gaccgagctg ggagccgctg
      121 ccgttaacct ggagggttgc ggcgcaggga gcggtgaagg acacgctgac cggctgcaac
      181 gtcacgtgct caatcccgaa gcggtcgtgc aattcccgct cggcgtcccc gtagaacgcg
      241 tcgctcggca gcccgtcggg catcaccagg tgcgcggtaa gcgcattctc cgaggtgccc
      301 atggcccaca catgaagacc agcgacccgc tcgacgcccg gcatggactc gataaatcgg
      361 tgaaccttgc gtatccgatc aacttccggt aaccgccctc attcggatac cagctcgaac
      421 tctttcgccg gctcctactt cggcgcggcg agcggcgcga accttctgca ggggcttcta
      481 gtccttgcgg ttctccttcg ccgcctcttg atgtccacat ccctgcggcg cggcttccgg
      541 aatccggcct ttccccagtc ttcgatactt ccgcgcaagc gcgatcaatt cggaatttcg
      601 cagctttcga aaatcgagcg acgcctgaca cagatcctcg cgcggaatgg tgcgaacgat
      661 cgcgagaatc gcggaatcgg cgatcacgtc gcggcccgct gtcagctctt cgcggtgaaa
      721 cgaatagaac tgagctccgc gatccgacag cgcgtagcgc acccaccggc cttctctggt
      781 ctcgattgcg agcccggcgt ccttcatgac ggcgagatga gtcgacacgg tcgaaaccgc
      841 ggagtgcagg acggtcgtga tctggcaaac atactgcggc tccttgccca gcatcgccag
      901 aatgcgcaga cgcagcgggt gcgcgagcgc cttgtgctcg tcaaccggtc cggcgagatc
      961 ggcatctatc atttcgaagt ttcacgaagc atgatgcatg tcgattcata cttcggattt
     1021 aacagaaata aagtgctaca tgtaaatatc tcagaataag tatgttgccg ccgtcaatct
     1081 acggagaaga ttctctgtcg agtcacggac gacgggtgat caccgccacc ccaattgtgt
     1141 tcgcgttgca cgtccgttcg gtgactcccg ctacgaaacc ctatctgcgg ccatggcacc
     1201 gttcgcacga ccttggattc ggactccgga tcatccctga tctcacggca aaaggagcga
     1261 tcatgcaccc gggcagtcaa cgagtggcgt ttcgtcttcg tcctctgctc ctgatgatag
     1321 gagccgtgct cattcccgcg caactgctgt acggcgcgaa cccatgcaga gactgccatg
     1381 aaaagcaggt cgccgcgatc gccgcgggtc ctcacgccgc ggtcgcggct cgcgacgcca
     1441 ggttctgcgc cacgtgccac ggagatccga aggctcacct cgaatccggc gacccggccg
     1501 acatcgttgg cgggaagaca ctcgccacgt ggcgcggcga gcgtcaggca cttgcctgcg
     1561 cgagctgtca cgagaaggag tttccggcct ggcgccaggc accgcacacc gcgaaccgac
     1621 tttgctggtc ctgtcacgcg tcgcaagcgc ttcactcgaa aggcgagcct ccgctggggc
     1681 cggcgaaagc ccaccaaacc tgggggctct gcatctcctg tcatccgggg gaggccgcgc
     1741 agttccggat ggtctaccgc catccggtgg agcgcggcgt catggactgt accgattgcc
     1801 acgacattca cggcagatcg gtactgcaga cgaatcgggt cgacgaccac gcgtgcctcc
     1861 gctgtcacga ggaacagaag ggtcccttcc tgttcgagca cgccgggatg gagcagggct
     1921 gttccaactg tcacgccgcg cacggttcac cacatcgcgg ccagttgctc agctccggca
     1981 acggagcctg cctgacatgt cacatccaga gcaacttccc ggggatcggc aagacctcgc
     2041 acgattctct tctgtccggc ggaggcaggt gctgggactg ccactccgag gttcacggat
     2101 caaacacgac acccgacttc aatccgcgag ggaggaggtt gaggcgatga aacagatact
     2161 ggccgcaggt tcggtcctcc ttgtcttctt gctcgcgcca ggcctgacgg cgcagaccag
     2221 cggaagcgtc gcggttggcg cgcaggcgga ctcgaccagc ggaaacgtct ctgcctttca
     2281 ggagcagaat ggaggcgagg agtccggcgt attcatcgag cgtctcaagc tcctcggcac
     2341 tggaacggtt ccatggcaga tcgacggccg cttcagcacc ggcggcagcg gatggctgga
     2401 cctgtcggct cgcgacggcg cctggagcgg tgggctgcgc ctgacgcagg cgagaacatg
     2461 gtccgcgaca tcgttcgcag acgacgccct gccgtccggc gtccccgtct cgtcgctcag
     2521 ccctccgtcg acgacgctcg atccgctctt cggagtcacc gatccgcatg gcgagctcct
     2581 gcacaccgag gcctggctga cgcgagccat cgggaacgcg tcgcgcctga ccgttcgcgc
     2641 cggctatcgc cagcgcgacg gctcacgcgt ccccaacatc ggcgccttct ccttcagcga
     2701 tgtcggtacc cccgcgttct tcgcgccggg actcgagcgg atcgattcat cctcgagttg
     2761 gggcgcgctc gaggcgacat ccttgtggcg cggccttgcc ttacaactcg acgccggagc
     2821 ctccaggcgc gacaaccgga ctcgctacga ccttcccgcc tacggcacga gcggtctgct
     2881 cgacttgaat cgctggacca cgggcacgga agcagactcc cgctggttcc gcctggcagc
     2941 ctcgcgtccg tcggagcgcg tcactgttta cggaggagtc agttggatcg acgtctcgtc
     3001 gactccgagc ggctcggacc ttcgcgtcac cggaaacggc gatgtcgttc gcgacggtct
     3061 gtcgctcgag aacggatcga tcgacgcgac cacgagcgca ggcgcggccg gattcacctg
     3121 gcgcatgcat cccgccgttg cactgacggt ttccgccgac gtccagtcac gctcatccga
     3181 gggaagcggc cggattcttc ttcgctcggc aaatcccgtt ttcacgacga ccgatacatc
     3241 gagcgaccgc gtcggcgcca ctgccgatct gacgggtcga ttcggcgcca cacgcgtcca
     3301 tctccgcggc cgggttacga cgaccaacat cgatcgcctt gaagactcga ctccatttct
     3361 gcaggacgaa acccgttcga tcgaccgtat ggagatccgc ggggatctgt cccgccgcct
     3421 gagcggggac atccgcgcac gcggatgggt gcgttatgcg gacgagtcgg gctcagtcga
     3481 tcttcgacag ctcgactacg gctatgcgag ctcggactgg gatcgcgacg atctctccgg
     3541 cggcatcgag ctcgcgctcg gcagtggaga ccggcgggcg aacctgacgg tcaatggtgg
     3601 caagtccgac gtcctcgaca ccccgccgct gttcgacccg gtctacgatc cctctctcga
     3661 gctctccgac atcggcggca ccatcagcac ctttcgtgcc gcagtcacag gcatgaggaa
     3721 tttcgctcgc ggcacggcgt ggggcgaggc cgggtggctt tcgaccgagt acgaatacaa
     3781 cgcgtcattc gatcagcccg gcttttcatc catgagcgag aagatcgaag gcctcgtggc
     3841 gtcggctgga gcggaagtcc ggccacgcga cggcacgcgc gtcatcggcc aggtcgaatg
     3901 ggtgcgcgac cgcgcggatc tcgatcgcac actgacgcgc gcctctctcg agctcgcgca
     3961 gacgattcac acacggttcg aattgttcgg acgctggttc gtgggagacc tcgacgcccc
     4021 ccacgcccag cagtccaact acagcgtcaa cctgttcgcc gtcggggtac ggacgaactt
     4081 ctgattggag ccagcgcatg agctacagaa acagccgttt cgccgccatc ggactcgcgc
     4141 ttgccgttct ctgcatcctc ggtcctggcg gtcttgcctc acccggcaag gcgaacgcgc
     4201 ccacggcgat gggtggcgcc gcagcgagca cgacgagtct gacgggaatc cacgatccgc
     4261 tgtcgcgcaa ctacgactcg gactgcgtca agtgtcacgc cagcatcctg aaggagagca
     4321 gccgcgaccc gcgcattctc gcctttcatc aggcgatgat cccgtacacc ccgggttaca
     4381 gcgcgcgaaa gggtgtgcag agcaaaaatt gcgtgacgtg ccatcgcgac gcgatcgact
     4441 ttcgtcagga gtcgggcagc tcgctccgcc gcaccgtgag catcgagtcg tgcgtctact
     4501 gccacggccg caccgggcct ggcccggcgt atttcgagtg aggagaacgt gatgacgcgc
     4561 aatggattcc tcgtcgacct caactactgc atttcctgca aagcctgtga agtggcgtgc
     4621 aagacgtgga acgccgttcc aaccgaccgc ggcatccgtt ggcgccgggt tgtcgatgag
     4681 ctgctcggcg tcgtcccgag gacgaccgcg tacagtgtct cgctcgcgtg caatcactgc
     4741 gagaatccgg cgtgtgtgcg cgcctgcccg aacggcgcgc tgaccatgcg ggcggacggc
     4801 ctggtcgttc acgacaagac gaagtgtgtc ggctgtcgct actgcgagtc ggtgtgtcct
     4861 tacggtgccc ctcaatacga cgcagtcgag aaagtcatga cgaagtgcac cggctgcttt
     4921 gaccgggtcg atcagggtct cctgccggcg tgcgttgatg catgtccggc ggaagcactc
     4981 aagttcggcc cgctggccga gatcgaccgc gaaggcattc gcgagatccc tcgatttcca
     5041 aacccgaatc gaaccaggcc ggcggtcagg ttcgttccga aggagagctg aaatgacgaa
     5101 gaacggattt tcgcggcgga cgtttctccg cacttcggga gtcgcggcgg cgggcctggt
     5161 ggccgcgccg gcattcctcg agagcctctt cgcccaaacg ttcggtcctc ttcagtcgct
     5221 tcccgcggga gtcacgcagc tcatcaccgc gtgcggcctc tgcgactccg tgtgtggcct
     5281 gcgagcgacc atgaaggacg ggatcctcac gttcgtcgag ggtgtgaaag aggatctcca
     5341 tggcggtggg aagctgtgcg ggaagggcaa gtcggctgcc ggcttcctct acgatcctga
     5401 caggctcaag tacccgatga aacggacgaa ccccatcaag gcgagcgggt ccgatccggg
     5461 atgggttcgg atcagctggg acgaggcgct ggggatggcc tcggagacac tgaaccagac
     5521 cattgcagcg cacggagcag actcggtgct cgtgatctcg cgtcccgcgc ctgacatctg
     5581 gatgcgtttc atcaatgcca tcggaacgcc caaccgcgtc gaccatatcg acgagtgctt
     5641 cctgagcgac aaaatcgtct ccggcaaagt actcggcggc aagctgtggg gacacgatct
     5701 ggccaactcg aagtacatcc tgctcttcgg ctgggatctc gtcgggaaag cgaagatggc
     5761 ctacgcgcgg caggtcgtga aggccaagaa ggccggcgcg aagatcgtgg tcttcaaccc
     5821 gatgtggacg cccacggcga cgctcgccga cgagtggcac cagatccgcc ccggctcgga
     5881 tctcgcgctc gcactggcga tgatccaggt gatcgtctcc gagaagctgt acgacgccga
     5941 gttcgtgaag agttacacga acttcccggc ttacgagcag cagtttcagg acaacttcgc
     6001 gccgtacacg ccggaatggg ccgcggaact gaccgacatt ccggcgcaca caatacggag
     6061 aatcgcccgc gagttcgcga tgacgaggcc ggggatcgtt ccaacccaca agaaaacatc
     6121 ggcagcgaac tacgcgaacg ccgcgcccct gcagcacgcg atcgcaatcc tgaattgcct
     6181 caatggcagc atcgacaggc ccggtgggcg cttctatccg cgccaggtcg agctcgacgg
     6241 tgtcgacaaa atctatccgc cgccggcgta tccggcgaag agcggagtgt ggatcgacgg
     6301 gcgggaccgg cacccgttcg tcaagggcgc gggcaacggg atgttctcga cgctggcgaa
     6361 cggaatgctc aaggtccatc ccgaaaagat tcaaatggcg atcatcacga agtacacgac
     6421 aatgggcttc ccgaacccgc ccgagatcgc cagcgccctg gcgaaggtgc cgttcacggt
     6481 cgtcgtcgac atcgtgccta acgagatcat cgacctggcc gacctcgtcc tgcctcaggc
     6541 cagctatctc gaatccgccg acatcgcggt gcgggagcac tatgcgcggt tcccgcaggc
     6601 gttcgtcagg cagccggtcg ccccggcgat gttcgagagc aagggcttcg gctggatcgc
     6661 gatcgagtgg ggcaagcggt cgtttcccga ctacttcaag aagcctgacg gctcctggct
     6721 caatatgaat gagatcctgg acgagaaggt ccaacgggcg aagctcgcgc cgtcctttgc
     6781 cgacttcaag aaagccgggt tctactcgga cgagcagccg ttcgttccga agacgacgtt
     6841 tcccacgccg gacggaaagc tccgtctctg ggtgcaggac ttcgctgaca agggcttcga
     6901 tccgcttccg aaatggatgc ccaagcggga tctgccgtcg gccgaatacc cgttctacct
     6961 gatcaccgtc attcccggtg tccacatccg caacacaacg cagaacaacc cgttcctcaa
     7021 cgagatcttc gggacgaact tcgtaatgat tcatccggag acggcgcgaa gactcgacat
     7081 cgaggagggg gacccggtac gcgtgaaatc gcgcgtggga accatcacgc tgccggcgcg
     7141 gatctcggga ggaatccgtc ccgactgtgt ctgcatccct cacggcttcg gtcatcgctc
     7201 gcgccttctc acgaaggcat acggaaaggg cgagagagac tccgatctcg tgccgtcgca
     7261 gagcatggac gagatcctgg ctcgtcgtga cgtgggcggc gccggggcga tcatggacgc
     7321 cgtcgtatct ctcgaaagga cggcctgagc gtgccgcttg cgccgctcct tcgtctttca
     7381 ctgaattcgg ctgccagcgg agagccgctg gc
//
LOCUS       DASWAV010000415         3699 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U2665
            k141_580042, whole genome shotgun sequence.
ACCESSION   DASWAV010000415 DASWAV010000000
VERSION     DASWAV010000415.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960316
            Sequence Read Archive: SRR10260007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 3699)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3699)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:47:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,929
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3699
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_580042"
                     /isolate="SMAG_U2665"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.36444 N 132.31889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1040
                     /locus_tag="VF958_08565"
     CDS             <1..1040
                     /locus_tag="VF958_08565"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF022137.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="class I SAM-dependent rRNA methyltransferase"
                     /protein_id="HEX9149197.1"
                     /translation="HSQIVARIWSFDGRPPDLGLFRERFAVARRLREEILPPETTGYR
                     GVNSEGDMCPGVLVDLFDEVGVMDLTTEGTVRWRGDLERAAAEAFNPAHIFVRESGVE
                     RDRARLAEATPSPRPSPGERGGRREPDLAPFLENGLRFFADVTGGQKTGFFLDQRENR
                     SRLRATARGRSVLDLFSYSGAFSVAALAGGAARAVDVDSSESALVLAREHRRANGFSV
                     AEEDFIRADVFEDLRRRVAAGESWDVVVCDPPAFARKRGDLSRAARGYQDVNRLAMSL
                     VAPGGWLLTCSCSGLVTADLFQKIVFSASLEAHRTFALAARQGAGPDHPISLDCPEGE
                     YLKGFWLRRET"
     gene            1037..2179
                     /locus_tag="VF958_08570"
     CDS             1037..2179
                     /locus_tag="VF958_08570"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX9149198.1"
                     /translation="MIERVALLGPAPPDRGGIARETALLARELEKRVALTWLTYSRPY
                     PAWLDPRRFDRHPNESAPASARPALDYRSPGSWKRTAREIVSTRPDALVAPWWTSFWG
                     LPLRSVLRKVRELSPDTARVLLCHNVRDHRSGVFERFLTLGAFFSADAFVVHAESDRE
                     RLARRFRGRPIVVMPHPVVDAPRPDRDRARRDLGVTAPLVLFLGLIRRYKGVDLLLDA
                     APRIVGDTGAKIAVVGEVFPEARFLERRLEASPVRDSILWRDDYVSEEEMARWLAAAD
                     AVVLPYRYVSGSGIAARAIAARRPLVAAAVGGLSQTVLPGSTGELFAPGDPDALAQAV
                     GRVLARGLQSYETGLTAAANERSWPRYADGLLSFLAAVKSRGRELR"
     gene            2193..3365
                     /locus_tag="VF958_08575"
     CDS             2193..3365
                     /locus_tag="VF958_08575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002639613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEX9149199.1"
                     /translation="MDFELTEDQALLHSAVREFAEEVVRPQAARIDQTGEFPRNVFAE
                     AGKLGLAGVSVAAEYGGSAMDTISYTIVIEEISRACANMGVILSVNNSLVCDPIAKFG
                     NEDQKTRFLTPLARGEKLGCFALTEPDAGSDAGNQKTRAVREGNVYRISGQKVFITCG
                     QAADVCLLFAMTQPEKGPRGISAFLVDAESPGFDRSHHQVKLGVNASGTVEIFLTDVR
                     VPVENRLGEEGEGFRIAMTTLDGGRIGIAAQAVGIATEACEAAVSYAKQRRAFGRPIA
                     DFQVIRFYLADMATDLDAARLLTRRAAAAKDAALARGGRFSLEAAIAKLFAAEMAQRV
                     TTKALQIHGGYGYTKEYPVERNFRDARITEIYEGTSEIHRLIIAREIFKDAGLAIG"
     gene            3382..>3699
                     /locus_tag="VF958_08580"
     CDS             3382..>3699
                     /locus_tag="VF958_08580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9149200.1"
                     /translation="MPQTRMFRDRVVSMEQTFEGGEKNLDLLKAICVEATPEFEYVAH
                     FLDHERDLFVMDFAAADGRKKRVSWTRMVLFDAERIPVLSGDPSGHLRGRIVQYLRSR
                     ANRP"
BASE COUNT          556 a         1275 c         1265 g          603 t
ORIGIN      
        1 cgcactcgca gatcgtcgct cggatctgga gcttcgacgg ccgaccgccc gacctcgggc
       61 tcttccgaga acgtttcgcg gtggctcgcc ggcttcggga ggagattctt ccgccggaaa
      121 cgaccggata ccggggcgtg aattcggaag gggatatgtg tccgggagtg ctcgtcgacc
      181 tgttcgacga ggtcggggtg atggacctga ccacggaggg taccgttcga tggcgcgggg
      241 atctcgagcg agcagccgcc gaagcgttca atcccgccca catctttgtg cgcgagagcg
      301 gagtcgaaag ggaccgtgcc cggttggccg aagcaacccc ctcaccccga ccctctcccg
      361 gtgagagagg ggggcgacgc gagccggatc tcgcgccgtt tctggagaac ggcctccgct
      421 tcttcgccga cgtcaccggc ggacagaaga ccgggttctt tctcgatcag cgcgagaacc
      481 gctcccgcct gcgcgcgacg gcgcgaggga ggtccgtcct cgatctgttc tcgtattccg
      541 gtgccttttc ggtcgccgcg ctcgccgggg gcgcggcgcg ggccgtcgac gtcgactcct
      601 ccgaatccgc gctggttctc gcgcgcgagc accggcgcgc gaacggcttt tcggtcgcgg
      661 aggaagactt catccgcgca gacgttttcg aggacctgcg acgccgtgtt gcggccggag
      721 agtcctggga cgtcgtcgtg tgcgaccccc cggcgttcgc gaggaagcgc ggcgacctct
      781 cacgcgcagc gcgcggctac caggacgtca atcgcctcgc gatgtcgctc gtcgccccgg
      841 gagggtggct gctcacctgc tcctgctccg ggctcgtcac agccgacctc ttccagaaga
      901 tcgtgttctc cgcgagcctg gaggcgcatc gcaccttcgc gctcgcggcg cggcagggcg
      961 cgggaccgga tcaccccatc tcgctcgact gtccggaagg ggagtacctg aagggcttct
     1021 ggctccggcg cgagacgtga tcgagagagt tgcgctcctc ggacccgccc cgcccgaccg
     1081 cggcgggatc gcgcgggaga cggcgctgct cgcgcgcgag ctcgagaagc gggtcgcgct
     1141 cacgtggctc acctactcgc gcccgtaccc cgcgtggctc gatccccggc gcttcgaccg
     1201 gcacccgaac gagagcgcgc cggcgtcggc gcgtcccgcg ctcgactacc gctcgcccgg
     1261 ttcctggaag cggaccgccc gggagatcgt ctccacccgc cccgatgccc tggtcgcgcc
     1321 gtggtggacg tcgttctggg gcctgccgct gcgcagcgtt ctccggaaag tgcgggagct
     1381 ctcgcccgat accgcccggg tgctcctctg ccacaacgtt cgagaccacc gatccggcgt
     1441 cttcgaacgc tttctcacgc tcggagcttt cttctctgcc gatgctttcg tggtgcatgc
     1501 cgagagcgat cgcgagcggc tcgcacgccg tttccgcgga cggcccattg tcgtcatgcc
     1561 gcatccggtc gtcgacgcgc cgcgaccgga ccgcgaccgc gcgcggcgcg acctgggggt
     1621 cacggcgccg ctcgtcctct ttctcggact catccggcgc tacaagggcg tggatctcct
     1681 gctcgacgcc gcgccccgca tcgtcgggga caccggagcg aaaatcgcgg tcgtcgggga
     1741 ggtctttccc gaagcgcgat tcctcgagcg gcgcctggag gcgagcccgg tccgcgactc
     1801 gattctctgg cgcgacgact acgtctccga ggaggaaatg gcgcgctggc tcgcggctgc
     1861 ggacgcggtc gttctgccgt accgctacgt ctccggcagc gggatcgcgg ctcgcgcgat
     1921 cgccgcgcga cggcccctcg tggccgcggc ggtcggcggg ctttcgcaaa cggtgctgcc
     1981 gggctcgacc ggcgagctct tcgcgccggg tgatcccgac gcgctcgcgc aagccgtcgg
     2041 acgcgtgctc gcacgcgggc tccagtccta cgagacgggc ctgaccgcgg cggcgaacga
     2101 acgttcgtgg ccgcggtacg ccgacggcct tctctccttt ctggccgccg tgaaatctcg
     2161 gggccgcgag cttcggtaga ctgcgaaacc ggatggactt cgagctcacg gaagatcagg
     2221 cgctcctgca ctccgcggtg cgcgagttcg ccgaagaggt cgtccggccg caggccgcac
     2281 gaatcgacca gacgggggag tttccccgca acgtcttcgc cgaggcgggc aagctcgggc
     2341 tggcgggagt cagcgttgcc gccgaatacg gcgggtctgc gatggacacg atctcctaca
     2401 ccatcgtcat cgaggagatc tcgcgggcgt gcgcgaacat gggcgtcatc ctgtcggtga
     2461 acaactccct cgtctgcgac ccgatcgcaa agttcggcaa cgaagaccag aaaacccgct
     2521 tcctgacgcc tctcgcccgc ggcgagaaac tcggctgctt cgcgctcacc gaacccgacg
     2581 ccggctccga cgccgggaac cagaagaccc gggcggtccg cgaggggaac gtctaccgga
     2641 tatcggggca gaaggtcttc atcacctgcg gccaggctgc ggacgtgtgc ctccttttcg
     2701 cgatgacgca gccggagaaa gggccgcggg ggatctccgc ttttctcgtc gacgccgaaa
     2761 gtcccggctt cgaccgctcc caccaccagg tgaagctcgg cgtcaacgcc tccgggaccg
     2821 tcgagatctt cctgaccgac gtgcgcgtcc ccgtcgagaa ccgtctggga gaagagggcg
     2881 aggggtttcg catcgcgatg acgaccctgg acggcggccg catcggcatc gccgcgcagg
     2941 ccgtggggat cgcgaccgag gcgtgcgagg ccgcggtgtc gtacgcgaag cagcgtcgtg
     3001 ccttcggccg gccgatcgcc gacttccagg tgatccgctt ctaccttgcc gacatggcga
     3061 ccgacctcga cgcagcgcgg ctcctgaccc ggcgcgccgc cgccgcgaag gacgcggccc
     3121 tcgcgcgggg cgggcgcttc tctctcgagg cggcgatcgc gaagctcttc gccgcggaga
     3181 tggcgcagcg ggtgacgacg aaggcgctcc agatccacgg cggatacggc tacacgaagg
     3241 agtacccggt cgagcgcaac ttccgcgatg cgcggatcac cgagatctac gaggggacga
     3301 gcgagatcca tcgcctcata attgcaaggg agatcttcaa agacgccgga ctggcgatcg
     3361 gataggagac cggctgaacc gatgcctcag acacgcatgt ttcgtgaccg ggtcgtctcg
     3421 atggagcaga ccttcgaggg cggcgagaag aatctcgacc tcttgaaggc gatctgcgtc
     3481 gaagcgacgc cggagttcga gtacgtcgcg cactttctcg accacgagcg ggatctcttc
     3541 gtgatggact tcgccgcggc agacggccgg aagaagcgcg tttcgtggac gcgcatggtc
     3601 cttttcgacg ccgagcggat cccggtgctc tccggagatc cgtcgggcca cctccggggc
     3661 aggatcgtcc aatacctgag atcgcgggcg aaccgtccg
//
LOCUS       DASWAV010000416        13765 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U2665
            k141_23559, whole genome shotgun sequence.
ACCESSION   DASWAV010000416 DASWAV010000000
VERSION     DASWAV010000416.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960316
            Sequence Read Archive: SRR10260007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 13765)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13765)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:47:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,929
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13765
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_23559"
                     /isolate="SMAG_U2665"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.36444 N 132.31889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..316)
                     /locus_tag="VF958_08585"
     CDS             complement(<1..316)
                     /locus_tag="VF958_08585"
                     /inference="COORDINATES: protein motif:HMM:NF014757.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase NAD-binding
                     domain-containing protein"
                     /protein_id="HEX9149201.1"
                     /translation="MKISRVGVLGAGLMGSGIAEVCAKAGYETIVREVSDELARKGVG
                     RVESSLAKAVERGKLAAADRDAARGLLTATTRVQDLEKCDLVIEAVVENLPAKKELFG
                     ELD"
     gene            complement(303..581)
                     /locus_tag="VF958_08590"
     CDS             complement(303..581)
                     /locus_tag="VF958_08590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9149202.1"
                     /translation="MLTWTERILSVPVRTAAVREFLRELSLRGWGDLNAMLATLNDGE
                     LCREALAYWVDKEWITPAPNSPLTALVGPAYTRELTLRQFCGETDEDQ"
     gene            complement(588..1787)
                     /locus_tag="VF958_08595"
     CDS             complement(588..1787)
                     /locus_tag="VF958_08595"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013938426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006635 - fatty acid beta-oxidation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase family protein"
                     /protein_id="HEX9149203.1"
                     /translation="MRVMASSSEIVILNGARTPMAEYNGSFAEVSAIDLAVVAAKEAL
                     ARSGTEPSEVDHVIFGNALQTSGDAIYGARHVGLKAGVPKEVPALTVNRLCGSGFESI
                     VQGAHRILLNEATTVLAGGMENMSQAPYVIRGARRGLKLGQGQLEDSLMVALLDSWSG
                     LYMAQTSDRVAAKYGISREEQDEYALSSQHRAAAAWAACRLSEEVVPVEAKAGRKSVR
                     VERDDHMRPDTTMEALAKLPPSFGKDGQVTAGNASGIVDGAAAVVVTSAENARRKGVK
                     PLGRLVSWGVVGCEPELMGLGPAPAARAALERAGLSKDDIDLWEINEAFAGQILGVVR
                     ELGLDREKLNVNGGAIALGHPLAASGTRLTLTLLKELSRRGARYGLAGACIGGGQGIA
                     VVVESLS"
     gene            complement(1823..2857)
                     /locus_tag="VF958_08600"
     CDS             complement(1823..2857)
                     /locus_tag="VF958_08600"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HEX9149204.1"
                     /translation="MTGLAEFLASEVGEGSFPGAVALVGDKDAVREIAAAGNAVLEPE
                     RIPVSEDTLFDLASLTKPLVCGALAVAALPSLDRTSSPGRYLPGWKATRYDGISLESL
                     LTHTSGLPAWYPVYSRGEGALAYRRTLATIEPEGRPSERVIYSDLNFLVFGDILEVHF
                     SAPLDATFAALVSGPAGSGASFLPGRSPSVAATEKGDLTERRMAEAMGISYPHFRGGV
                     VWGEVHDGNAYRRGGVAGNAGLFGTARDVWRLARPWLDSSRGDFTRDRTPGLPEARGL
                     AWQGSRGAGSAVPAMPDSAFGHTGFTGTSLWIDPEAGQIAVLLTNRVHPEARPVDFNA
                     VRRRFHERLW"
     gene            complement(2854..3408)
                     /locus_tag="VF958_08605"
     CDS             complement(2854..3408)
                     /locus_tag="VF958_08605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9149205.1"
                     /translation="MTPGRRALVLLVAGAVGLFCLSCRRERTGGGAAPTSGPPAAAGP
                     LRPQFQPPANRLLTDRHLDVYVKVRRAARDRPEADAARALGIDSDEFAWVRARVVEAL
                     VALDEAHVRQQSSETYARTIASLRQTLKSVRDPETARTVEAQIAGLERERTGMRASDP
                     LPADVAANARKVAGRRAELEAPPK"
     gene            3540..5387
                     /locus_tag="VF958_08610"
     CDS             3540..5387
                     /locus_tag="VF958_08610"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEX9149206.1"
                     /translation="MPEETPLPEKIGKYEITGKIGSGGFGAVYSGRDPYIKRTVAIKT
                     CQLNDEEIKSRFFREAELAGNLHHRHITTIYDFGVENGIPFIVQEFLSGEDLDKKIKR
                     ADPLPLYRKVEILAAITDGLAYAHTAGIIHRDIKPANVRILEDGSVKIMDFGIAKSLQ
                     SESNLTQTGITLGTSAYLAPEQIRGEPVDPRTDIFSLGILAYELLTYRKPFRGEHLST
                     VLYKILNETPEPIEAVAPDAPPALVAAVNRAMEKQRESRYASMDALKQDLLKVGRELA
                     GASSQFPTAVLPADEMDATVMTPSGGVASSITPPSGSLARVPAADATPSSSSSARRKA
                     GLELVNFRDPSAEEGEATVSGRSSEFAALAAAETRPASRTPIFIAIAAVVVAGIAVFF
                     YLNRGKSTEPAPPPPPAAAAAKPGAPAPIEFPEPQLGADAKKGAPAGAAEAKAKPREI
                     AAAPAASAEEPPPAAVAKRHVQFSSIPVATLFVDGKQIGPSLPAKSLELADGTHRVRF
                     EADGLEPYTREFKVGAKESNTIAYQFPVGYIVINADAAWSGAAVIIGGKYKGSLPQAS
                     RLRMPKGTYSVTLHREGFQPVTQQITVPERGESPWSPPPPVAVDQGMGQ"
     gene            5384..7015
                     /locus_tag="VF958_08615"
     CDS             5384..7015
                     /locus_tag="VF958_08615"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX9149207.1"
                     /translation="MKRAGAALTAFLLLSSVVLAQAEKLESEAKRAFDSGRFKEAAEK
                     YAKAAESPDAAAERKGDLYLNSAWAFYIAGNTKNSRDNLRAAYSARPDLVVVGDLYSP
                     DFARLAQSVRAEIAGTTPLLGPSELRELKRSARRNLTDGKAEAALAELKPASTSNDPE
                     IQLLLAEVYEKLGRTAEADAARQRASDLQKGLVTTTPISGSAPGAGTTAPIEPTVNVL
                     PLLDAADKALKAGDLAGATTHARRAADLDPKSAEAHRLLGDVALAGGQEADAEREFTA
                     AVVLDGSNSHAEMGLARLSERQKKWNTAASHYRRVLQLDPHSVGAAMGLGRSLQEAGD
                     DSAARLAYGRAIEIDASSPEAHNDFGVLLYRAGDTDQAIEHFSEAAKLRPDEPAYHEN
                     LGRAYRKKGMAKEAETELASAARLGTPTAPLWATLGRLRVEQKNPEEARAAFGSALAA
                     DPGNEEAASGLAAALYETGKLEQAESVLVKSIESNPGSSVLWNDLGVVRMRRSDFAGA
                     VEAFRKALSLEKPPEASKANLDRAEQLLALDRAAS"
     gene            7032..7919
                     /locus_tag="VF958_08620"
     CDS             7032..7919
                     /locus_tag="VF958_08620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011556669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1684 domain-containing protein"
                     /protein_id="HEX9149208.1"
                     /translation="MLLLVALLSLAAAPPTVKNDASSYRAEIEKWRADRAARLTADEG
                     WLSVVGLSWLEEGENSLGSVPSARIVLPAGSAPARLGTVRLAQGKATLTVGPGATVTH
                     DGKPVKTIDLTADADGEPTVLRHGTLTFYLIRRGDRLGMRVKDSAAAARKSFHGIDGY
                     PIDLAWRLPARFVAYQPKKSIPIPNVLGNVTQEPSPGAVVFSIGGKEYRLDAVEEAGE
                     EDLFLIFGDRTNGTETYGAGRFLYAPKPDSDGKTVVDFNRSYNPPCAFTAFATCPLPP
                     PQNRLPIAVTAGEKRYGQH"
     gene            complement(7925..8476)
                     /locus_tag="VF958_08625"
     CDS             complement(7925..8476)
                     /locus_tag="VF958_08625"
                     /EC_number="3.6.1.55"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005777 - peroxisome [Evidence IEA];
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003986 - acetyl-CoA hydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0008413 - 8-oxo-7,8-dihydroguanosine
                     triphosphate pyrophosphatase activity [Evidence IEA];
                     GO_function: GO:0010945 - coenzyme A diphosphatase
                     activity [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0035539 - 8-oxo-7,8-dihydrodeoxyguanosine
                     triphosphate pyrophosphatase activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0009132 - nucleoside diphosphate metabolic
                     process [Evidence IEA];
                     GO_process: GO:0015938 - coenzyme A catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA pyrophosphatase"
                     /protein_id="HEX9149209.1"
                     /translation="MNWMDDVERRLAAAARSGVPPGSARRAGVLVPLFVREGMLWVVF
                     TRRTDTVAHHRGQISFPGGAEEPGDEDLSRTALRETEEELGVRPDDVKILGKLSPMVT
                     VTDFYVEPYVAAIPQPYVFTPAESEIAEVVEAPIGALTNPAIKETKFLPDREEPVLFY
                     HYRDQVIWGATARMLAELLEALE"
     gene            complement(8473..9592)
                     /gene="prfB"
                     /locus_tag="VF958_08630"
     CDS             complement(join(8473..9516,9518..9592))
                     /gene="prfB"
                     /locus_tag="VF958_08630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_141201661.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HEX9149210.1"
                     /translation="MTERDDLISSLTDASGRLAALRGYLDRDAARSALDLLRQKTSAP
                     DFWSRPEEAQETLRQIHTHEKVLETGDRLDELADEVSTFADLLREGEPADADAREALA
                     AAREFADGLELAVKMGTPDDSKNAWVMIHPGAGGTESQDWAEMLLRMYTRFGEQRGWK
                     AELIERQDGDEAGIKSATLHVVGDHAYGYLKSEMGVHRLVRISPFDAAKRRHTSFASV
                     YVSPEVDEDIEIDISDKDLRIDTYRSSGAGGQHVNVTDSAVRITHLPTGIVVTCQNER
                     SQGRNRDMAMKVLRARLYQKALDERRAEEEKRVGDKKSIEWGSQIRSYVLHPYRMVKD
                     HRTGYETGNTDAVLDGAIAPFIEAYLKSAADSKSLEAR"
     gene            complement(9589..11112)
                     /gene="lnt"
                     /locus_tag="VF958_08635"
     CDS             complement(9589..11112)
                     /gene="lnt"
                     /locus_tag="VF958_08635"
                     /inference="COORDINATES: protein motif:HMM:TIGR00546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006497 - protein lipidation [Evidence
                     IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="apolipoprotein N-acyltransferase"
                     /protein_id="HEX9149211.1"
                     /translation="MKRDRWRSPVAGVVSGILFALAFPQYELPLLAPLALVPWIVALT
                     VEEKRFRGLVSGFLFGMAYWLVSIPWIVYVVTRFGSQSSAMGILSLVSVATIVSEWPA
                     VVGWAVVAVAPVRSVRRLAVFPFLWLASEHVRANVYRGFPWNLTAEALYRHPVWLQSA
                     AFWGAYGVGFLVVSVSALIAAGIVTRGLRPFLAAALLVLAVGTYGAVRLASRPDPAAR
                     TVTAALLQPNIPQESRLRPDRRAANYAEVIEQARAAAAAHPDLILIPESALPVYWETS
                     AILRRDLTDLARSCNCAVLFNDVDIETNDRYYNAARLLTPRGMGPPYRKVHLVPFGEY
                     VPLPRIFFFVRQVSTEIGEFSAAPEPTVLRGDGLSIGTGVCYEILYPVLSWKQVREGA
                     NLLVTISNDSWYGAGGAQAQHFAGAVLRAVENERYLLRAAITGISGVADEKGRILSEL
                     RPDEKGTIRTPVRLFTARTPWTRYGFAFSGLCDAIAIGVLLFGLVRWRSERRGRNRR"
     gene            complement(11123..12028)
                     /gene="lipA"
                     /locus_tag="VF958_08640"
     CDS             complement(11123..12028)
                     /gene="lipA"
                     /locus_tag="VF958_08640"
                     /EC_number="2.8.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF004019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016992 - lipoate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009107 - lipoate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HEX9149212.1"
                     /translation="MSEPLPASPLSPAGRAPEWIREKKLGLGQLHAVKKLARFHALST
                     VCEEARCPNRGECFSRGTATFLMLGTVCTRPCGFCDIANGRPAPPDPFEPARVASAAR
                     EMALGYVVLTSVARDDLPDGGAGHFVAAIRALRGLAPPPGVEVLVPDFRGRFESLRSV
                     VEERPDVFNHNVETVARLYARVRRGARLDRSLGMLAAAKELAPDLTTKSGLMVGLGET
                     RQEVLDLLARLREAAVDVVTIGQYLRPARENLPVEEYVPPAVFEEYRVAGEAMGFRHV
                     FSGPFVRSSYRAEEALHAAHAAHET"
     gene            complement(12025..12669)
                     /locus_tag="VF958_08645"
     CDS             complement(12025..12669)
                     /locus_tag="VF958_08645"
                     /inference="COORDINATES: protein motif:HMM:NF016895.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HEX9149213.1"
                     /translation="MSEPPTHYQVSFTSRQALSLFVGLLAVLGLSYFFGLMTGLSGNE
                     VPVGGAAAAPAAGNAAAESGPPSPTSPPASQGAGGGEENLTFPVPVTAVRSAGGPRPG
                     PATTIHTFEDGEASASATTPVPARAATAKPGPASGGFRIQVLSVSSRADADAEAARLT
                     RGGLPARVEPGNGPRGTVYRVRVGPYATREEAEQAGRRLSTQGRRDTWIVPPGQ"
     gene            complement(12700..13473)
                     /locus_tag="VF958_08650"
     CDS             complement(12700..13473)
                     /locus_tag="VF958_08650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013008136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="slipin family protein"
                     /protein_id="HEX9149214.1"
                     /translation="MPQEIFSKTGLLVALFIGILFLFKWINVLNEYERAVTFWLGRLG
                     KKPKGPGLVLIFWPFERMVRISLRTVVHDVPPQDIITRDNVSVKVNAVVYFRVVEPDK
                     AVVQVENYLYATSQLSQTTLRSVLGQAVLDELLSEREKLNVALQQVIDQHTDPWGIKV
                     SLVEVKQVDLPPEMQRAMAKQAEAEREKRAKIIHASGEFEAARMLSEAAKIIGEEPAT
                     LQLRYLQTLTEIASEKNSTIIFPLPIEFMRVFMAKSEKG"
     gene            complement(13473..>13765)
                     /locus_tag="VF958_08655"
     CDS             complement(13473..>13765)
                     /locus_tag="VF958_08655"
                     /inference="COORDINATES: protein motif:HMM:NF014062.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HEX9149215.1"
                     /translation="YRIDLSIIVPALVLTLAVVGFLTWKTLEIRMRPARTGGEGMVGQ
                     TARVVEAFSPGSGKVFLHGEYWDADGPAGLAPGEVVRIAGVDGLRLRVERRD"
BASE COUNT         2255 a         4859 c         4638 g         2013 t
ORIGIN      
        1 ggtcgagctc tccgaagagc tccttcttcg cggggaggtt ctcgacgacc gcctcgatca
       61 cgagatcgca cttttcgagg tcctgcacac gggtcgttgc cgtgagaagc ccgcgcgccg
      121 cgtcgcggtc ggcagcggcg agcttgccgc gctcgactgc cttcgcgagc gaggactcga
      181 cccggccgac cccctttcgc gccagctcat cgctgacctc gcgcacgatc gtctcgtaac
      241 ccgccttcgc gcagacctcc gcgatcccgg agcccatgag cccggccccg agcacaccga
      301 ccctactgat cttcatctgt ctctccgcaa aattgccgca gcgtgagttc ccgcgtgtac
      361 gccggtccga cgagcgccgt cagaggcgag ttcggtgcgg gcgtgatcca ctccttgtcg
      421 acccagtacg cgagagcctc tcggcagagc tccccgtcgt tcaacgtcgc gagcatcgcg
      481 ttcaggtcgc cccatccgcg gagggaaagc tcgcgcagga actcgcgaac cgcggccgtc
      541 cgtactggaa cggagaggat cctttccgtc caggtcagca agctcgtcta cgaaagagac
      601 tcgacgacca cggcgatccc ctgccccccg ccaatgcacg cgccggcgag gccgtaacgg
      661 gcgccgcgcc gggagagctc tttcagcagc gtcagcgtca gccgcgtccc gctcgccgcg
      721 agcgggtggc cgagcgcgat tgctccgccg ttgacgttca gcttctcccg atcgaggccc
      781 agctcgcgca cgacacctag gatctggccc gcgaacgcct cgttgatctc ccagagatcg
      841 atgtcgtctt tcgaaagccc cgctctttca agcgcggcac gcgcggccgg cgccggtccg
      901 aggcccatga gctcgggctc gcagccgacg accccccaag aaacgagccg gccgagcggt
      961 ttgacgccct tgcgcctcgc gttctccgcg gaggtgacga ccaccgccgc ggcgccgtcc
     1021 acgattcccg acgcgttgcc ggcggtgacc tgcccgtcct tgccgaacga cggcggcagc
     1081 ttcgcgagcg cctccatcgt cgtgtccggg cgcatgtggt cgtcccgctc gacgcggacg
     1141 ctctttcggc cggcctttgc ctcgaccggc acgacctcct ccgagagacg gcaggccgcc
     1201 cacgccgcgg ccgcgcggtg ctgcgacgac agagcgtatt cgtcctgctc ctcgcgggag
     1261 atcccgtatt tcgcggcgac gcggtcggag gtctgtgcca tgtacaggcc gctccagctg
     1321 tcgaggaggg cgaccatgag cgagtcttcg agctggccct gcccgagctt gagaccccgt
     1381 cgcgcccccc ggatcacgta cggcgcctgc gacatgttct ccataccgcc ggcgagtacg
     1441 gtcgtcgctt cgttcaagag gatgcggtgg gcgccctgca cgatcgactc gaagcccgag
     1501 ccgcacaacc ggttcacggt cagcgccggg acctctttgg ggacgccggc cttcaagccg
     1561 acgtggcggg cgccatagat cgcgtcgccg gaggtctgga gggcgttccc gaagatgacg
     1621 tggtcgacct cggacggctc ggtcccggat cgggcgagcg cctccttcgc agcgacgacc
     1681 gccagatcga tcgccgacac ctccgcgaag ctcccgttgt actccgccat gggggtccgg
     1741 gcaccgttca ggatcacgat ttcggagctc gaggccatca ctcgtatcta taccacgcga
     1801 gggtccgggg attcgtcgcc cgtcaccaca ggcgttcgtg gaagcggcgg cggaccgcgt
     1861 tgaagtccac cgggcgcgcc tcgggatgaa cgcggttggt caggaggacg gcgatctgcc
     1921 cggcctcggg gtcgatccag agagacgtgc cggtaaatcc cgtgtggccg aaggcggagt
     1981 ccggcatcgc cgggaccgcg gaacccgctc cgcggctccc ctgccacgcg agcccccgcg
     2041 cctccggaag ccccggcgtc cgatcgcggg tgaaatcccc gcgggaggag tcgagccagg
     2101 ggcgagcgag gcgccagacg tcgcgcgccg tgccgaaaag cccggcgttg cccgcaacgc
     2161 cgccgcggcg gtacgcgttt ccgtcgtgga cctcccccca gacgacgccg ccccggaaat
     2221 gcggataaga aatccccatc gcctcggcca tccgccgctc ggtcaggtcg cccttctcgg
     2281 tcgcggcgac ggagggggac cggcccggaa gaaacgacgc gcccgacccg gccggcccgg
     2341 acacgagcgc tgcgaacgtc gcgtcgagcg gagcggaaaa gtggacctcg aggatgtcgc
     2401 cgaagacgag gaaattcagg tcgctgtaga tcacccgctc ggacggccgc ccctcgggtt
     2461 cgatcgtcgc gagcgtccgc cggtacgcga gagcgccttc gccgcgcgag tagacgggat
     2521 accaggcggg caggcccgac gtgtgcgtca ggagcgactc gagcgaaatg ccgtcgtagc
     2581 gggtggcctt ccaccccggg agatatcggc ccggcgagga ggtccggtcg agcgacggaa
     2641 gcgcagccac cgcgagtgcg ccgcagacga gcggcttggt gaggctggcg aggtcgaaca
     2701 gagtgtcttc ggaaaccggg atcctctccg gctcgaggac tgcgttcccg gcagcggcga
     2761 tctcccgcac cgcgtccttg tctccgacga gtgcgaccgc acccgggaag gagccctccc
     2821 cgacctcgga ggcgaggaat tctgcgagac ccgtcacttg gggggggctt cgagctcggc
     2881 ccttcggccc gcgaccttcc gggcgttggc cgcgacgtct gccggcagcg gatcggaagc
     2941 ccgcatcccg gtgcgctccc gctccagtcc cgcgatctgg gcctcgacgg tgcgcgcagt
     3001 ctccgggtcc cggacgctct tcaacgtctg acgcagggag gcaatcgtcc gggcgtaggt
     3061 ctccgaggac tgctgccgaa cgtgggcttc gtcgagcgcg acaagggcct cgacgacacg
     3121 ggcgcgaacc caggcgaact cgtccgagtc gatgccgagc gcccgggccg catccgcttc
     3181 cgggcggtcg cgcgccgccc gccgtacctt cacgtagacg tcgaggtggc gatcagtcag
     3241 gagacggttc gcgggcggct ggaattgcgg gcgaagcggc cccgcggccg cgggagggcc
     3301 ggacgtcggg gcagctccgc cgcccgttcg ctcgcggcgg caggagaggc aaaagagtcc
     3361 gacggccccg gccaccagca gaacgagcgc gcgacggccc ggtgtcattc gaccgagtct
     3421 atcggcccgc ggacacccag aatttccgcc ccgacggtgt gttgtgaagt tctccggctc
     3481 cgttcgcatt tctcttgcct cgtgattcga ttaaacttgc cgcgccgctc gaaaaggaaa
     3541 tgcccgaaga aaccccgctg ccggaaaaga tcggcaaata cgaaatcacc ggaaagatcg
     3601 gttccggcgg cttcggcgcc gtctacagcg gacgcgaccc gtacatcaag cggaccgtcg
     3661 caatcaagac gtgccagctg aacgacgagg aaatcaagag ccgcttcttc cgcgaagcgg
     3721 agctcgccgg gaaccttcac caccggcaca ttacgacgat ctacgacttc ggcgtcgaga
     3781 acggcattcc gttcatcgtc caggaattcc tctccgggga ggacctggac aagaagatca
     3841 agcgcgccga ccccctccct ctgtaccgca aggtcgagat cctcgccgcg atcacggacg
     3901 gcctcgctta cgcccacacc gccgggatca tccaccgcga catcaagccc gccaacgtgc
     3961 gcattctcga ggacggctcc gtcaagatca tggacttcgg aatcgccaag tccctccagt
     4021 ccgagtcgaa cctgacccag accgggatca cgctcggcac ctcggcatac ctcgcccccg
     4081 agcagattcg cggcgagccg gtcgacccgc ggacggacat cttctccctc gggattctgg
     4141 cctacgagct gctcacgtac cggaagcctt tccggggcga gcacctgtcg acggtcctgt
     4201 acaagatcct gaacgagacg cccgagccga tcgaggcggt cgcgcccgac gcgcctcccg
     4261 cgctcgtcgc ggcggtcaac cgcgcgatgg aaaagcagcg cgagagccgc tacgcgtcga
     4321 tggacgcgct caagcaggac ctgctcaagg tcggtcgcga gctcgccggt gcgtcgtccc
     4381 agttcccgac ggcggtcctc cccgcggacg aaatggacgc cacggtgatg acgccgagcg
     4441 gcggcgtcgc ttcttcgatc actccccctt ccggctctct cgcccgggtg cccgcggccg
     4501 atgccactcc gtcgtcctct tcttccgcgc gccgcaaggc gggactcgag ctcgtcaact
     4561 tccgcgaccc gagcgcggag gagggggaag cgaccgtctc gggccgtagc tcggagttcg
     4621 ccgcgctcgc agccgccgag acccggccgg cgagccggac cccgatcttc atcgcgatcg
     4681 ctgccgtcgt cgtcgccggg atcgcggtct ttttctactt gaatcgcggc aagtcgaccg
     4741 agccggcccc gccgccgccg ccggccgccg ccgcggcaaa accgggggcc ccggctccga
     4801 tcgaattccc ggagccgcag ctcggagccg acgcgaagaa gggcgcgccc gccggcgccg
     4861 ccgaggcgaa agccaaaccc cgggagatcg ccgcggcccc cgccgcttca gcggaggagc
     4921 cgccgccggc agcggtcgcg aaacggcacg tgcagttcag ctcgattcca gttgcgacgc
     4981 tgttcgtgga cggcaagcag atcggacctt cgcttcccgc gaagtccctc gagctcgccg
     5041 acggtacgca ccgggtgcgc ttcgaagccg acgggctcga gccctacacc cgggagttca
     5101 aggtcggcgc caaagagtcg aacacgatcg cgtaccagtt cccagtcgga tacatcgtga
     5161 tcaacgccga cgccgcctgg agcggtgcgg cggtgatcat cggcggtaag tacaagggca
     5221 gcctgcccca ggcgtcgcgg ctgagaatgc cgaaaggcac atacagcgtg acgcttcacc
     5281 gggaaggatt ccagcccgtg actcagcaga tcaccgttcc cgagcgcggc gagagcccgt
     5341 ggagtccgcc gccgccggtc gccgtcgatc agggcatggg acagtgaaga gggccggcgc
     5401 ggctctgacc gcgttcctcc ttctttcttc ggtcgtcctc gcgcaggcgg agaagctcga
     5461 gtcggaggcg aagcgggcat tcgactccgg gcggttcaaa gaggccgccg agaagtacgc
     5521 gaaggccgcc gagtcgcccg acgctgcggc cgaacgcaaa ggcgatctct acctgaacag
     5581 cgcctgggcg ttctacatcg ccggaaacac gaagaactcg cgcgacaacc tgcgagccgc
     5641 ctattcggcg cgcccggacc tcgtcgtcgt cggagacctc tacagccccg acttcgcgcg
     5701 cctcgcgcag tcggtccgag cggagatcgc ggggacgacc ccgctgctcg gtccctccga
     5761 gctgcgcgag ttgaaacgtt cggcgcgtcg caacttgacc gatggaaagg ccgaagccgc
     5821 gctcgcggag ctgaagcccg cctcgacgtc gaacgatccc gagatccagc ttctcctggc
     5881 ggaggtctac gagaagctcg gccgaaccgc ggaggccgac gccgcgcggc agcgcgcctc
     5941 ggatctgcag aaagggctcg tcacgacgac tccgatctcg ggaagcgcgc cgggcgcagg
     6001 gacgacggcc ccgatcgagc cgacggtcaa cgttctgccg ctcctggacg ccgcggacaa
     6061 ggctctcaag gcgggcgacc tcgcgggcgc gaccacccac gcgcgccggg ccgcggatct
     6121 cgacccgaag agcgcggaag cccaccgcct gctcggcgac gtcgcgctcg cgggagggca
     6181 ggaggccgat gccgagcggg agttcaccgc cgcagtcgtt ctggacgggt cgaattcgca
     6241 cgcggaaatg ggcctcgcgc gcctgtccga gcgccagaag aagtggaaca cggccgcgag
     6301 ccactaccgg cgcgtcctgc agctcgatcc ccacagcgtg ggggcggcaa tgggtttggg
     6361 ccggtccctt caggaggccg gcgacgacag cgcggcccgc ctcgcgtatg ggcgcgcgat
     6421 cgagatcgac gcgtcgtcac ccgaggcgca caacgacttc ggcgtactgc tctatcgcgc
     6481 gggagacacg gaccaggcga tcgagcactt ctccgaagcg gcaaagctac ggcccgacga
     6541 accggcctac cacgagaatc tcggacgggc gtaccggaag aaaggcatgg cgaaggaggc
     6601 cgagacggag ctggcgtctg cggcccgtct cgggacgccg acggcgccac tgtgggcgac
     6661 cctcggccgc ctccgcgtcg aacagaagaa ccccgaggaa gcgcgcgcgg cgttcggctc
     6721 ggcgctcgcc gcggaccccg gcaacgaaga ggccgcgagc gggctcgcgg cggcgctgta
     6781 cgagacgggc aagctcgaac aggcggagtc ggtgctcgtc aagtcgatcg aatcgaaccc
     6841 cggctcttcc gtgctctgga acgacctcgg cgtcgtgcgg atgcgccgga gcgatttcgc
     6901 cggtgcggtc gaggcgttcc gcaaggcgct ctcgctcgag aaaccgccgg aggcgtcgaa
     6961 ggcgaacctc gatcgcgccg agcagctgct cgccctcgat cgcgccgcga gctgacgcac
     7021 tccccacgcc ggtgctcctc ctcgtcgcgt tgttgtcgct cgccgcggct cctccgaccg
     7081 tgaagaacga cgcgtcgagc tatcgcgccg agatcgagaa gtggcgcgcc gaccgggctg
     7141 cgcgcctcac cgcggacgag ggctggctct cggtggtcgg gctctcctgg ctcgaagagg
     7201 gagagaactc gctcgggtcc gtgccgtcgg cccggatcgt ccttcccgcc ggaagcgccc
     7261 cggcgcggct gggaaccgta cgtctcgcgc agggaaaggc gacgctgacg gtcgggccgg
     7321 gtgcgacggt gacgcacgac gggaaacccg tgaagacgat cgacctcacg gccgacgcgg
     7381 acggagagcc gacggtcctc cgccacggga cgctcacctt ctatctcatc cgtcgcggcg
     7441 accggctcgg gatgcgggtg aaggacagcg ccgccgccgc gcggaagtcg ttccacggga
     7501 tcgacggtta cccgatcgat ctcgcgtggc gactgccggc ccgattcgtc gcgtaccagc
     7561 cgaagaaatc gatcccgatc ccgaacgtgc tcgggaacgt gacccaggag ccgtccccgg
     7621 gcgccgtcgt cttctcgatc ggcgggaagg agtaccgact cgacgcggtc gaggaagcgg
     7681 gcgaagaaga tctattcctg atcttcggcg accgcacgaa cggaaccgag acctacggcg
     7741 cggggcgatt cctctatgcg ccgaaaccgg actcggacgg aaagaccgtc gtcgacttca
     7801 acaggtccta caacccgccg tgcgcgttca ccgcgttcgc gacgtgtccc ctgccgccgc
     7861 cgcagaaccg actcccgatc gccgtcaccg ccggcgagaa gcggtacggg cagcactagg
     7921 gtctttactc caaagcctcc agcagctccg ccagcatccg cgccgtcgct ccccagatca
     7981 cctggtcgcg gtagtggtag aagagcacgg gctcctcgcg gtccggcagg aacttcgtct
     8041 ccttgatcgc aggattggtg agcgcgccga taggcgcctc cacgacttcc gcgatctcgc
     8101 tttcggccgg cgtgaacacg tagggctgag ggatcgcggc gacgtacggc tcgacgtaga
     8161 agtcggtcac cgtcaccatc ggcgagagct tgcccaggat tttcacgtcg tccgggcgca
     8221 cgccgagctc ctcctcggtc tcgcgaaggg ccgtccgcga gaggtcttcg tcccccggct
     8281 cctccgcgcc ccccggaaac gagatctgtc cccggtggtg cgcgacggtg tccgtgcggc
     8341 gcgtgaagac gacccagagc attccttctc gcacgaagag ggggacgagc acgcccgctc
     8401 gccgggccga accgggggga acgcccgacc gggcggccgc cgcgagccgc ctctcgacgt
     8461 catccatcca gttcatcggg cttcgagcga cttcgagtcg gcggcggatt tcagataggc
     8521 ctcgatgaac ggcgcgatcg ctccgtcgag cacggcgtcg gtgttcccgg tctcgtaccc
     8581 ggtgcgatgg tccttgacca tgcggtaggg atgcagaacg tacgagcgga tctggctccc
     8641 ccactcgatc gacttcttgt caccgacgcg cttttcctcc tccgcgcgcc gctcgtcgag
     8701 cgccttctgg tagaggcggg cgcgcagcac cttcatggcc atgtcgcggt tgcgtccctg
     8761 gctgcgctcg ttctggcacg tcacgacgat cccggtcgga aggtgcgtga tccgcacggc
     8821 ggagtccgtg acgttcacgt gctgcccgcc ggcgccgctc gaacggtacg tgtcgatccg
     8881 caggtccttg tccgagatgt cgatctcgat gtcctcgtcg acctctggcg agacgtaaac
     8941 ggaggcgaag gatgtgtgac gacgcttcgc ggcgtcgaac ggcgagatcc gcacgagccg
     9001 gtgaactccc atctcgctct tcaggtatcc gtacgcgtgg tcgccgacga cgtgcagcgt
     9061 ggcgctcttg atcccggcct cgtccccgtc ctgccgttcg atcagttctg ccttccagcc
     9121 gcgctgctcg ccgaaccggg tgtacatgcg gaggagcatc tcggcccagt cctgtgactc
     9181 ggtgccgccg gcgcccgggt ggatcatcac ccacgcgttc ttcgagtcgt ccggcgtccc
     9241 catcttgacc gcgagctcga ggccatcggc gaactcccgc gccgcggcca gcgcctcgcg
     9301 cgcgtctgcg tcggcgggct cgccctcgcg caggagatcc gcgaacgtcg agacctcgtc
     9361 cgcgagctcg tctaaccggt cccccgtctc caggaccttc tcgtgggtgt ggatctgccg
     9421 gagggtctcc tgcgcctcct cgggccgcga ccagaagtcg ggcgcggagg tcttctgcct
     9481 caggaggtcg agcgcgctcc gcgcagcgtc gcgatcaaag gtagccgcgg agcgccgcca
     9541 ggcgtcccga ggcgtccgtg agtgaggaga tcaagtcgtc gcgttcggtc atcgtcgatt
     9601 tcgcccgcga cgctcggagc gccagcgaac gaggccgaag agtagcacgc cgatcgctat
     9661 cgcgtcgcag aggcccgaga acgcgaaccc gtaccgcgtc cacggggtgc gcgccgtaaa
     9721 gaggcgcacg ggcgtccgga tcgtcccctt ctcgtcgggc cggagctccg agaggatccg
     9781 ccccttctcg tccgcgacac cggagatccc cgtgatcgcg gcccgcagca ggtatcgctc
     9841 gttctcgacc gcgcgcagga cggcgccggc gaagtgctgg gcctgcgcac cacccgcgcc
     9901 gtaccaggag tcgttcgaga tcgtcacgag caggttggct ccctcgcgaa cctgcttcca
     9961 cgagagcacc ggatagagga tctcgtagca gacgccggtc ccgatcgaca ggccatcgcc
    10021 gcgaaggacc gtcggctccg gcgcggcgga gaactcgccg atctcggtcg acacctgccg
    10081 gacgaagaag aagattctcg gcagcggcac gtattcgccg aagggaacga ggtgcacctt
    10141 gcggtacggc ggccccatgc cgcgaggcgt cagaagccgg gcggcgttgt agtagcgatc
    10201 gttcgtctcg atgtcgacgt cgttgaacag gaccgcgcag ttgcaggaac gggcgaggtc
    10261 cgtcaggtcg cggcgcagga tcgcgctcgt ctcccagtag acgggaagcg ccgactccgg
    10321 gatgaggatc aggtccgggt gcgcggcggc cgccgcccgc gcctgctcga tcacctccgc
    10381 gtagttcgcc gcccttcgat ccggccggag ccgcgattcc tgcgggatgt tcggctgcag
    10441 gagcgccgcc gtcacggtgc gggcggcggg gtcgggtctc gaggcgaggc ggactgcccc
    10501 gtacgtcccg accgcgagga cgaggagcgc cgccgcgagg aacgggcgca aaccccgggt
    10561 cacgatgccg gcggcgatga gcgccgacac gctgacgacc aggaaaccga cgccgtaggc
    10621 gccccagaaa gccgcgctct ggagccagac cggatgtcgg tagagcgcct ccgccgtcag
    10681 gttccatgga aaaccgcggt agacgttggc gcgtacgtgc tcggacgcga gccagaggaa
    10741 ggggaaaacg gcgagcctcc ggaccgagcg cactggagcg acggccacga ccgcccagcc
    10801 gaccacggcc ggccattcgg agacgatcgt cgccacgctg acgagggaga ggatccccat
    10861 cgcgctcgac tggctcccga agcgagtgac gacgtagacg atccacggga tcgagacgag
    10921 ccagtaggcc atgccgaaga gaaacccgga gacgaggccg cgaaaccgct tctcttcgac
    10981 cgtgagcgcg acgatccagg gcacgagcgc gaggggcgct aagagcggga gctcgtactg
    11041 cgggaacgcg agagcgaaca ggattccgga gacgacgccc gccacaggcg agcgccagcg
    11101 atctcgtttc acgttttccg ttttacgttt catgcgccgc gtgagcggcg tgaagcgcct
    11161 cttccgcgcg atacgaagac cgcacgaagg gtccggagaa gacgtgccga aaccccatcg
    11221 cctcccccgc gacgcgatac tcctcgaaga ccgccggggg cacgtactcc tcgaccggca
    11281 gattctcgcg agcggggcgc agatactggc cgatcgtcac gacgtcgacc gcggcctcgc
    11341 gcagccgggc gagcaggtcg agcacctcct gccgcgtctc gccgaggccg accatcagcc
    11401 ccgacttcgt cgtcagatcc ggcgccagct ccttcgcggc agcgagcatt ccgagagacc
    11461 ggtcgagccg ggcgccccgc cgcactcgcg cgtagaggcg agcaacggtc tcgacgttgt
    11521 ggttgaagac atcgggccgc tcctcgacga cggagcgaag cgactcgaag cggccacgaa
    11581 aatcgggaac gagcacctcg acgcccggcg ggggcgccag gccgcgcaaa gcgcggatcg
    11641 ccgcgacgaa gtgtcccgcc ccgccgtcgg ggagatcgtc gcgggcgacc gacgtcaaga
    11701 cgacgtagcc gagcgccatc tcgcgggcag cgctcgcgac ccgcgcgggc tcgaaggggt
    11761 ccggcggcgc cggccgtccg ttggcgatat cgcagaagcc gcacgggcgc gtgcacaccg
    11821 tgcccagcat cagaaaggtc gccgtccccc gggaaaagca ctccccccgg ttcgggcagc
    11881 gcgcctcttc gcaaacggtc gagagcgcgt ggaagcgggc gagcttcttg accgcgtgga
    11941 gctgcccgag tccgagcttc ttctcgcgga tccactccgg ggcgcgcccg gccggcgaga
    12001 ggggcgacgc gggaagtggc tcgctcactg tccgggcggc acgatccagg tgtcgcggcg
    12061 accctgggtc gagaggcgac ggcccgcctg ctccgcctcc tcgcgcgtcg cgtacggccc
    12121 gacccgcacg cgatagaccg taccccgcgg tccgttcccc ggctcgacgc gagcggggag
    12181 cccaccgcgc gtcaggcggg cggcttccgc gtccgcgtcc gcgcgcgaag agaccgaaag
    12241 aacctggatc cggaaaccgc cggaagccgg acccggcttc gccgtcgcgg cccgcgcggg
    12301 aacaggcgtg gtcgccgacg cggaggcttc gccgtcctcg aacgtgtgga tggtggtcgc
    12361 ggggccgggg cggggaccgc cggccgatct caccgccgtg accgggacag ggaacgtcag
    12421 gttctcttcg ccgccgcctg cgccttgcga ggccggtggc gacgtcggac tcggagggcc
    12481 gctttccgcc gccgcgttcc cggccgcggg cgccgcggca gcgccgccga ccgggacttc
    12541 gttgccggag agcccggtca tgaggccgaa gaagtacgag aggccgagca ccgcgaggag
    12601 gccgacgaag agcgagagcg cctgcctcga cgtgaacgag acctggtagt gcgtcggcgg
    12661 ctcgctcacg ccgctagcct acgcgccggg cgctccgcgt caaccctttt ccgacttcgc
    12721 catgaacacc cgcatgaact cgatcggcag cgggaagatg atcgtcgagt tcttttcgct
    12781 ggcgatctcc gtcaacgtct ggaggtagcg cagctgaagg gtcgcgggct cctcgccgat
    12841 gatcttcgcc gcctccgaca gcatccgggc cgcttcgaac tctccggatg cgtggatgat
    12901 cttcgcccgc ttttcccgct cggcttcggc ctgcttggcc atcgcgcgct gcatctccgg
    12961 cggcaggtcg acctgcttga cttccacgag cgagaccttg atcccccacg ggtccgtgtg
    13021 ctgatcgatc acctgctgga gcgcgacgtt cagcttctcg cgctccgaca gaagctcgtc
    13081 gaggaccgcc tggccgagca cgctccgcag cgtcgtctgc gagagctgcg acgtcgcgta
    13141 caggtagttc tcgacctgga cgaccgcctt gtccggctcg acgacgcgga agtagacgac
    13201 cgcgttgacc ttcaccgaca cgttgtcgcg ggtgatgatg tcctgcggag ggacgtcgtg
    13261 cacgacggtc cgaagcgaga tgcggaccat cctctcgaac ggccagaaga tgagcacgag
    13321 cccggggccc ttcggcttct tcccgagtcg gccgagccag aacgtgaccg cccgctcgta
    13381 ctcgttcagg acgttgatcc acttgaagag aaacaggatt ccgatgaaga gcgcaaccag
    13441 caaaccggtc ttcgagaaga tctcctgggg catcagtccc tccgttcgac gcgaagccgc
    13501 aatccatcca cgccggcgat tcggaccacc tcgcccggcg cgaggccggc cggtccgtcg
    13561 gcgtcccagt attcgccgtg caggaacacc tttcccgacc cgggcgagaa ggcctcgacc
    13621 acgcgcgcgg tctgcccgac catcccctcc ccgccggtcc gcgcgggacg catccggatc
    13681 tcgagcgtct tccacgtgag gaacccgacg accgcgagcg tcagcacgag cgcgggcacg
    13741 atgatcgaga ggtcgatccg gtaga
//
LOCUS       DASWAV010000417         4147 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U2665
            k141_544671, whole genome shotgun sequence.
ACCESSION   DASWAV010000417 DASWAV010000000
VERSION     DASWAV010000417.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960316
            Sequence Read Archive: SRR10260007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 4147)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4147)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:47:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,929
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4147
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_544671"
                     /isolate="SMAG_U2665"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.36444 N 132.31889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(224..1009)
                     /locus_tag="VF958_08660"
     CDS             complement(224..1009)
                     /locus_tag="VF958_08660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018291194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX9149216.1"
                     /translation="MWNRIAALLLRYLYLYRRSLARSMEIFFWPVMDLLVWGFVTVYL
                     RSVAAPKPVLFLLGAVIFWDVFYRSQQAITLSLSEEIWVRNLLNIFISPVRIPEIVLA
                     TCLLGLIRALASAAVLGALAYAFYAFNLTVLGAALFPYLTSLLLFGWAVGMCTMAVIL
                     RFGHAAEALIWGTPFLLQPISAAFYPVSVLPPWLQTVARLLPSTHVFEGMRATLLTGR
                     ADLAPLVPAFALNVVYLVVAGVFFGWMLMRVREKGYLGRLAME"
     gene            complement(1012..1785)
                     /locus_tag="VF958_08665"
     CDS             complement(1012..1785)
                     /locus_tag="VF958_08665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008945587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX9149217.1"
                     /translation="MRPLSESASNAVLEVEHLTKRFDGVTAVDDVSFSARPGEILGLL
                     GPNGAGKTTTIQLLLGLTTPTSGSIRLFGLELPRHRREILQRVNFSSAYISLPFNLSV
                     RENLNVFARLYGVRDRERRIRELLELFEIAEVAATVTGKLSSGQVTRVNLCKAFLNDP
                     EVLFLDEPTASLDPDIAEKVRGALKLVQRERGVTIIYTSHNMHEVELVCDRVIFLSRG
                     RLVAEGTPREIIEQADKESLEQVFITIARSGDAPRTEDR"
     gene            complement(1904..2851)
                     /locus_tag="VF958_08670"
     CDS             complement(1904..2851)
                     /locus_tag="VF958_08670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020741297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB N-terminal domain-containing protein"
                     /protein_id="HEX9149218.1"
                     /translation="MPPRKRKKSAEPASRGLAARELASRDVPAEVAKLEREIESDGGA
                     ALAVFRDPLGGRWQILAGLPIDQVAPTPFQRDVSPAHVTRLAAVIDRLDRFLDPIIAV
                     RTPEGTYWTPNGHHRLSALRALGARSVTALVLPDPELAYKILALNTEKAHNLREKALE
                     TIRMARDLAEKDPSAPETAYALEFEEPAFLTLGLCYEKNGRFSGGAYHPVLKKIDEFF
                     DSPLAKALERRKERAETVLELDGTVNEAVAALKERGMQSPYLRAFVVARVNPLRFQRG
                     TTRPAFDATIGKMLTAARNFKAGSVKMDQLARAGGPPEE"
     gene            2880..3863
                     /locus_tag="VF958_08675"
     CDS             2880..3863
                     /locus_tag="VF958_08675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012829222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine dehydratase"
                     /protein_id="HEX9149219.1"
                     /translation="MTSGKWPIDLRDVLEARLRIRPYLPPTPLRNYAALDRAAGNGVR
                     IWVKHENHNPTNSFKARTAMSVLTALPEDARRRGVLGATRGNHGQGLAWAGALLGAPV
                     VVCVPIGNNPEKNEAMRGLGAELVEEGRDYDESVEVARRIVRERGVTMVHSTNDPLVI
                     AGAATLTLEILEERPRIDAIVVAVGGGSQAVGALTVVRALEPGIRVYGVQAERASAIH
                     DSWHAGRVLKKDTADTFADGLATRQPYEGTFPSLQEGLSGFVAVSESEIAEAVRTLLK
                     TTHNLAEGAGAASFAGVGRLASELAGKDVAVVLSGGNIDSATLTRVMTHEL"
BASE COUNT          723 a         1346 c         1420 g          658 t
ORIGIN      
        1 gggagacgat cggttacgtg aagaagatcc tgtcgtggtg gacgccggcg atcgccgcga
       61 ccgtcgcccc cacgatctcc gcggggggct cgacgccgta gggggtcagg tctccacttg
      121 ccactttggg ggtcaggtct ccacttgcca ctttgctgcg caaagatggc aaggttgctt
      181 tctcgattcg agcgtcttcc gtatcagtga agacctgacc cgcttactcc atcgccagcc
      241 gtccgaggta gcccttctcc cgaacccgca tcagcatcca gccgaagaag actcccgcca
      301 cgacgaggta gacgacgttc aacgcgaagg ccgggacgag cggcgcgaga tccgcccgtc
      361 ccgtcaggag agttgcgcgc atcccctcga aaacgtgcgt cgacggaagg agtcttgcga
      421 cggtctgcag ccacggcggc aggaccgaga ccggatagaa ggccgccgat atcggctgga
      481 ggagaaatgg agttccccag atgagcgcct cggccgcgtg cccgaagcgg aggatcacgg
      541 ccatcgtgca catccccacc gcccagccga agagaaggag gctcgtcagg tacgggaaga
      601 gcgccgcccc gagcaccgtc aggttgaacg cgtagaaagc gtaggcgagg gcgccgagca
      661 cggcggcgga cgcgagcgcc cggatcaggc cgaggaggca cgtcgcgagg acgatctccg
      721 gaatccgcac cggagagatg aaaatgttca ggagattccg gacccagatc tcctccgaca
      781 acgacagcgt gatcgcctgc tgcgatcggt agaacacgtc ccagaagatc accgcgccga
      841 gcaggaagag gacgggcttc ggcgcggcga cgctccgcag gtagaccgtc acgaaccccc
      901 agaccagcag gtccatcacc ggccagaaga agatctccat cgaacgggcg aggctgcgcc
      961 ggtagaggta gaggtagcgc aggagcaggg cggcgattcg attccacatc gtcagcggtc
     1021 ctccgtccgc ggcgcgtctc cggagcgcgc gatcgtgatg aagacctgct cgagcgattc
     1081 cttgtccgcc tgctcgatga tctcccgggg cgttccctcc gcgacgaggc ggccgcgcga
     1141 caggaagatc acgcggtcgc aaacgagctc cacctcgtgc atgttgtgcg acgtgtagat
     1201 gatcgtgacg ccgcgctccc tctggacgag cttcaacgcg ccccggacct tctccgcgat
     1261 gtccgggtcg aggctggccg tgggctcatc gaggaagagc acctcggggt cgttcaggaa
     1321 tgctttgcac aggttgactc gggtgacctg ccccgacgag agcttgcccg tgacagtggc
     1381 ggcgacttct gcgatctcga agagctcgag gagctcgcgg atgcgccgtt ctcgatcgcg
     1441 aaccccataa aggcgggcga aaacgttcaa gttctcgcgt acggagagat tgaacggaag
     1501 cgaaatgtac gcggaagaga agttcacgcg ctggaggatc tcgcggcggt gcctgggaag
     1561 ttccaggccg aagagccgga tggagcccga cgtcggagtg gtcaacccga gaaggagctg
     1621 gatggtcgtc gtcttccctg cgccgttggg gccgagcagc ccgaggatct ctcccggccg
     1681 ggccgagaaa gagacgtcgt cgacggcggt cactccgtcg aaccgtttcg tcaggtgctc
     1741 gacctcgagg acggcgttcg acgcgctctc gctcagtggt cgcactcgaa cttcgaaacc
     1801 ggcccgtcta tcctgcgagc gcgacgaccc gcaccgagtc ggcagcgtga gagacacccg
     1861 tcagcgcggt aacgagaaag aggatcctcg aaatccgcat ggcctattcc tccggggggc
     1921 ctccggcgcg cgcgagctgg tccatcttga ccgagcccgc tttgaaattc cgcgccgccg
     1981 tgagcatctt tccgatcgtc gcgtcgaacg cgggccgcgt cgtcccgcgc tggaatcgca
     2041 gggggttgac ccgggcgacg acgaacgcgc gcaggtacgg gctctgcatc ccgcgctcct
     2101 tcagcgcggc gacggcttcg ttgaccgtgc cgtcgagctc gaggaccgtt tccgcccgct
     2161 ccttccggcg ctcgagcgcc ttggcaagag gcgagtcgaa gaactcgtcg atcttcttca
     2221 gaaccgggtg ataggcgccc cccgagaatc ggccgttctt ctcgtagcag aggccgagcg
     2281 tcaggaacgc cggctcctcg aactcgagcg cgtaggccgt ctccggagcg gaggggtcct
     2341 tctcggcgag gtcgcgcgcc atccggatcg tttcgagcgc cttctcgcgc aggttgtgcg
     2401 ctttctcggt gttgagcgcg aggatcttgt acgccagctc ggggtccggc agtacgagag
     2461 ccgtgaccga cctcgcgcca agcgcgcgca acgccgagag ccggtggtgg ccgttcgggg
     2521 tccagtacgt cccctcgggc gtccgcaccg cgatgatcgg gtcgaggaac cggtcgaggc
     2581 gatcgatcac cgccgcgagg cgcgtgacgt gggcggggga gacgtcccgc tggaacgggg
     2641 tgggcgcgac ctgatcgatc gggaggcccg cgagaatctg ccatcggccg ccgagcggat
     2701 cccggaaaac ggcgagggcc gccccgccgt ccgactcgat ctcgcgttcg agtttcgcca
     2761 cttcggcggg cacgtcccgg gaggcgagct ccctcgccgc gagtccgcgt gaggcgggct
     2821 ccgcgctctt cttccttttc cgcggcggca tcgcaggctc gcgcctatat tgtcacgcga
     2881 tgacttccgg aaagtggccg atcgatctcc gggacgttct cgaagcgcgg ctccggatcc
     2941 ggccctatct cccgccgacg ccgctgcgca actacgccgc cctcgaccgc gccgccggga
     3001 atggagttcg gatatgggtg aagcacgaga accacaaccc gacgaactcc ttcaaggcga
     3061 ggaccgcgat gtcggtcctg acggcgctcc ccgaggacgc gcgcaggcga ggggttctcg
     3121 gagcgacgcg cggcaatcac ggtcagggcc tcgcctgggc cggagcgctg ctcggcgcgc
     3181 ccgtcgtcgt ctgcgtcccg atcggtaaca atccggagaa gaacgaagcg atgcgcggct
     3241 taggggcgga actcgtcgag gagggtcgcg actacgacga gtcggtcgag gtcgcgcgcc
     3301 ggatcgtccg cgagcgcggc gtcacgatgg ttcattcgac gaacgacccg ctcgtgatcg
     3361 cgggagcggc gacgctgacg ctcgaaatac tcgaagagcg gccgcggatc gatgcgatcg
     3421 tggtcgccgt cggcggcggg tcgcaggcgg tgggagcgct gaccgtcgtt cgtgcgctcg
     3481 agcccgggat ccgcgtgtat ggcgttcagg ccgagcgcgc gtcggcgatc cacgactcgt
     3541 ggcacgcggg ccgggtcctc aagaaggata ccgccgacac gttcgccgac ggcctcgcga
     3601 cccgccagcc gtacgaaggg acctttccgt cgcttcagga gggcctttcc ggattcgtgg
     3661 ccgtcagcga atcggagatc gccgaagcgg tccgcacgct cctcaagacg acgcacaacc
     3721 tcgccgaggg cgcgggggcg gcgagcttcg cgggagtcgg gcggctcgcg agcgaactcg
     3781 cggggaagga cgtcgccgtc gtcctgtcgg ggggcaacat cgattccgcg acgctcaccc
     3841 gcgtgatgac gcacgagtta tgagccgagt cactcgaccc atgtggcaag tggagacctg
     3901 accccgagac ctgaccccga atcccgtcgt tatactcccg ccgaaatcga aatcaacgaa
     3961 ctgcgggagg acgcgtgaag tctctgctcc gggctctgtc gatcttcggt ctgctggcga
     4021 cgtcgccgct cctctttgcg gcggcgccga agaagaattc tccggtcggc acggaggagg
     4081 gcaagtcctt cgccgatctc ttcccgggac tcgagttccg atgcatcggt ccgtaccgag
     4141 gcggccg
//
LOCUS       DASWAV010000418         2670 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U2665
            k141_342145, whole genome shotgun sequence.
ACCESSION   DASWAV010000418 DASWAV010000000
VERSION     DASWAV010000418.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960316
            Sequence Read Archive: SRR10260007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2670)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2670)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:47:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,929
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2670
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_342145"
                     /isolate="SMAG_U2665"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.36444 N 132.31889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1129)
                     /locus_tag="VF958_08680"
     CDS             complement(<1..1129)
                     /locus_tag="VF958_08680"
                     /inference="COORDINATES: protein motif:HMM:NF028046.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HEX9149220.1"
                     /translation="MRFAFRLNLILVLLAAGLATLLSVVAVRLTDRAVEDRARDRLRR
                     EIDLLADEVEARLPQPDRVDEFVRRAAERLGVRVTVIATDGRVLDESGVSPSDVPAME
                     NHAARPEVVQARAEGYGTSVRRSATLEADLLYLARPIGADRTGGPILRLAIPLAELRR
                     IEAKYEWILGGAVLVACALLVVAGSVAVARLARPIRDVTDAALAVARGDLGREGPEAG
                     PQEILELSSALKRMKGSLLQSVERAEGERRLAAAVFETLPDGVVVVDDKLRVLDANGG
                     FRAMMSAPDPVGRPLVDLLRDRALFAPFDDALARHEPVDATVRRPRDATWQVAVRPLP
                     PGSRGAVVGILRDVTALERNEAMRRRFVADVSHELRTPVASI"
     gene            complement(1151..1864)
                     /locus_tag="VF958_08685"
     CDS             complement(1151..1864)
                     /locus_tag="VF958_08685"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX9149221.1"
                     /translation="MNTPERVVSRRIALVEDDADIAYTIRLNLRKHGWQVDHFISGLA
                     ALEAIEEKPYDLVILDLNLPDVDGLAVARELRQHEETKRISILMLTARVEERDKLLGF
                     DVGADDYLTKPFSMKELVARVSAQLRRAGALDSPGDEIYETAGLRIDRARFEVSVDGN
                     PVRLTKREFDLLWYLVRNRARVVTRDTILARLWGYDADVETRTVDVHIRSLRRKLGER
                     WIDTVVGVGYRFVEGRAEG"
     gene            complement(1880..2644)
                     /gene="pstB"
                     /locus_tag="VF958_08690"
     CDS             complement(1880..2644)
                     /gene="pstB"
                     /locus_tag="VF958_08690"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015335301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HEX9149222.1"
                     /translation="MLDQPSRLETVNLDFFYGARQALFDVSLAVPEMSVTALIGPSGC
                     GKSTFLRCLNRMNDVIPNTRVKGKVLLDGEDLYAAGVDVVHLRRRVGLVFQKSNPFPK
                     SIFDNVAYGLRINGIGRKSELPEQVERALQRAALWDEVKDRLRAPALSLSGGQQQRLC
                     IARALAVEPEVLLMDEPASALDPIATQKIEELIYELKTAITIVIVTHNMQQAARVSDR
                     TAFFMMGRLIEYDRTDRIFTNPGQKVTEDYITGRFG"
BASE COUNT          444 a          904 c          904 g          418 t
ORIGIN      
        1 cgatcgacgc gaccggcgtc cgcagctcgt gggagacgtc cgcgacgaat cgccgccgca
       61 tggcttcgtt gcgctcgagc gcggtcacgt cccggaggat ccccacaacg gcgccccgtg
      121 agcccggtgg aagcggcctc acggcgacct gccacgtcgc gtcgcgcggc cggcgaaccg
      181 tcgcgtccac cggttcgtgg cgcgcaagtg cgtcatcgaa gggagcgaaa agcgcgcgat
      241 cgcgcaggag atccacgagc gggcgtccga ccggatcggg cgccgacatc atcgctcgga
      301 agccgccgtt ggcgtccagg acccgcagct tgtcgtccac gacgacgacg ccgtccggca
      361 acgtctcgaa caccgccgcc gcgagtcggc gctcgccctc cgctcgctcg acggattgca
      421 gaagcgatcc cttcatccgc ttgagcgcgg acgagagctc gaggatctcc tgcggaccgg
      481 cctcgggccc ctcgcggccg aggtctccac gggcgacggc gagcgccgca tccgtgacgt
      541 cccggatcgg tcgcgcgaga cgagcgaccg cgacgctacc cgcgacgacg agcagggcgc
      601 aggcgacgag caccgcgccc ccgaggatcc actcgtattt cgcctcgatg cgcctcagtt
      661 cggcaagcgg gatcgcgaga cgaagaatcg gcccgccggt ccgatcggct ccaatcggcc
      721 gcgcgaggta gagcaggtcc gcctcgagcg tcgcggaccg ccggacggac gtgccgtaac
      781 cttcggcccg ggcctggacg acctcgggcc gcgcggcgtg gttctccatg gccggcacgt
      841 cgctcgggga aacgccggat tcgtcgagga cgcgtccgtc cgtcgcgatc acggtgacgc
      901 ggacgccgag cctctcggcc gcccggcgga cgaactcgtc cacgcggtcc ggttgcggga
      961 gacgagcttc cacctcgtcg gcgaggaggt cgatctcgcg ccggagccgg tcgcgggcgc
     1021 ggtcttcgac cgcgcggtcc gtcagacgca cggcgaccac cgacagcagc gtggcgaggc
     1081 cggccgccag gaggacgaga atcaggttca accggaaagc gaagcgcatg gtggaagccg
     1141 cgcggcggtc ttaaccttcc gcgcgcccct cgacgaagcg atatccgacg ccgaccacgg
     1201 tgtcgatcca gcgctccccg agcttgcggc gcagcgatcg gatgtggacg tcgacggtgc
     1261 gcgtttcgac gtcggcgtcg tagccccaga gccgcgccaa aatcgtgtcg cgcgtgacga
     1321 cgcgcgccct gttgcgcacg agataccaga ggaggtcgaa ctcccgttta gtgagccgga
     1381 cggggtttcc gtcgacggaa acctcgaatc gcgcccgatc gatccgaaga ccggccgttt
     1441 cgtagatctc gtctcccggc gagtcgagcg ctccggctcg acgcaattgg gcggagactc
     1501 gcgccacgag ctccttcatc gaaaacggtt tcgtcaggta gtcgtcggcg ccgacgtcga
     1561 aaccaaggag cttgtcgcgc tcttccacgc gcgcggtcag catcaggatc gagatgcgct
     1621 tcgtctcctc gtgctgccgg agctcgcgcg ccacggcgag cccgtcgacg tcgggcaggt
     1681 tcaagtcgag gatgaccagg tcatacggct tctcctcgat cgcttcgagc gcggcgagcc
     1741 ccgagatgaa gtgatccacc tgccagccgt gcttgcggag gttcaaccgt atcgtgtagg
     1801 cgatgtcggc atcgtcttcg acgagcgcga tccggcgcga gactaccctc tccggagtgt
     1861 tcatcggaga tccgcccatc tagccgaacc ttccggtgat gtagtcctcg gtgaccttct
     1921 gccccgggtt cgtgaagatc ctgtcggtcc ggtcatattc gatgaggcgt cccatcatga
     1981 agaaagcggt ccggtcggaa acgcgggcag cctgctgcat attgtgcgtc acgatcacga
     2041 tcgtgatcgc ggtcttgagc tcgtaaatga gctcctcgat cttctgggtc gcgatcggat
     2101 cgagggcgga cgccggctcg tccatgagga gcacctccgg ctccacggcg agcgcgcgcg
     2161 cgatgcatag cctctgctgc tggcccccgg acagggagag cgcgggcgcc cgaagacggt
     2221 ccttgacttc gtcccagagc gccgcgcgct ggagcgcgcg ctcgacctgc tcggggagct
     2281 cggacttccg gccgattccg ttgatgcgca agccgtaggc gacgttgtcg aagatcgact
     2341 tggggaacgg gttcgatttc tggaagacga gcccgacccg ccggcggagg tgaacgacgt
     2401 cgacgccggc cgcgtagaga tcctctccgt cgaggagaac cttccccttc acgcgagtgt
     2461 tggggatgac gtcgttcatt cggttcaagc accggaggaa cgtcgacttc ccacatcccg
     2521 aaggtccaat gagcgcggtg accgacatct cgggcaccgc gagcgagacg tcgaacagcg
     2581 cctgtcgcgc accgtagaag aagtcgagat tcaccgtctc gaggcgagaa ggttggtcga
     2641 gcagcgcggc ggaggcagcc ccctcacctc
//
LOCUS       DASWAV010000419         2119 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U2665
            k141_782025, whole genome shotgun sequence.
ACCESSION   DASWAV010000419 DASWAV010000000
VERSION     DASWAV010000419.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960316
            Sequence Read Archive: SRR10260007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2119)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2119)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:47:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,929
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2119
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_782025"
                     /isolate="SMAG_U2665"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.36444 N 132.31889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            212..841
                     /locus_tag="VF958_08695"
     CDS             212..841
                     /locus_tag="VF958_08695"
                     /inference="COORDINATES: protein motif:HMM:NF025343.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEX9149223.1"
                     /translation="MASSRPTPRETERRVALLRAAFREVGEKGFSEVTLDDIAHRAGV
                     SKGVTLYYFDSKEDLFRELFAWLIDSIHARMRDAVESTDDPREKVRALVALIFPSPSK
                     NRAFFRAFVDFCGLAARRESFREVNERFYSGCFEIDGGIVEEGMRRGVFPVRDPKATA
                     STMRAIFDGLMMQWLAEKDPEASFLSYRERCERELLNYLTCGASDTRKG"
     gene            845..1015
                     /locus_tag="VF958_08700"
     CDS             845..1015
                     /locus_tag="VF958_08700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9149224.1"
                     /translation="MEKDVKEKEKCANPACGGKAAAGREYCDNCELEWTLYRRDLRGA
                     DREKEGPALESR"
     gene            1015..1986
                     /gene="ispH"
                     /locus_tag="VF958_08705"
                     /gene_synonym="lytB"
     CDS             1015..1986
                     /gene="ispH"
                     /locus_tag="VF958_08705"
                     /gene_synonym="lytB"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015898361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051745 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate reductase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA];
                     GO_process: GO:0050992 - dimethylallyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HEX9149225.1"
                     /translation="MFLRRVLLAAPRGFCAGVVRAIDVVNLALECVDAPLYVRREIVH
                     NLHVVRGFEKRGVRFVEELDEVPDGATVVFSAHGIAPRVRDEARRRGLNVIDATCPLV
                     TKVHLEAIRFERRGFRIILIGHPNHEEVEGTMGEAESRMTLVSSIAEAESVEVEDPDR
                     VAYITQTTLSMEDTRPIVETLKRRFPGIVGPARDDICYATQNRQIAVRALAREAPVIL
                     VIGSKNSSNSNRLVEEAQLAGARAYLVDDASGVDPAWLEGVETVGVTSGASAPEFLVE
                     QMVAHLTALGAERVDDLVTVEEDVHFPLPGELLEARQRRGTVALPTI"
     gene            1983..>2119
                     /locus_tag="VF958_08710"
     CDS             1983..>2119
                     /locus_tag="VF958_08710"
                     /inference="COORDINATES: protein motif:HMM:NF012334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX9149226.1"
                     /translation="MIRRLDGKVALVTGASRGIGEAIVRRFGADGARVALAARDVQAC
                     ER"
BASE COUNT          355 a          685 c          732 g          347 t
ORIGIN      
        1 ggaaagcgcg agaagccggg cacgtcccgc gcgagctcct gccagagctc gccgacgcgg
       61 ctcccggaag ggagttcccg agcctcttcg gcccgcccgg cagcgtcgcg aaacgaggcg
      121 aaatacagga cgcggacctc catcggtgag gagtctactt tcgccggagt ggcttgacat
      181 ctgaccgacc ggtcagatag tattttgggt catggccagc agccgcccca ctccccgcga
      241 gaccgagcga cgcgtcgctc tcctgcgggc cgccttccgc gaggtcgggg agaagggctt
      301 ctccgaggtg acgctcgacg acatcgccca ccgggcgggg gtttccaagg gcgtgacgct
      361 ctattacttc gattcgaaag aggatctctt ccgagagctc ttcgcctggt tgatcgactc
      421 gattcacgcg cgcatgcgcg acgcggtcga atccaccgac gacccgcgcg agaaagtgcg
      481 cgccctcgtg gcgctcatct ttccgtcacc gtcgaagaac cgcgccttct ttcgagcctt
      541 cgtcgacttc tgcgggctcg ctgcgcgccg cgagtcgttc cgcgaggtca acgagcgctt
      601 ctattccggc tgcttcgaaa tcgacggcgg gatcgtcgag gagggcatgc gtcgcggtgt
      661 gtttccggtt cgagacccga aggccacggc gtcgacgatg cgcgcaatct tcgacggtct
      721 catgatgcag tggctcgcgg agaaggaccc ggaagcgtcc ttcctctcct accgggagcg
      781 gtgcgagcgc gagctcttga actacctgac ctgcggcgct agcgacacga ggaaggggta
      841 acccatggag aaagacgtga aagagaaaga aaagtgcgcg aacccggcct gcggcgggaa
      901 ggccgccgcc ggacgagagt actgcgataa ctgcgagctg gagtggacgc tctaccgccg
      961 ggatctgcgc ggcgctgacc gggaaaaaga gggacccgcc ttagaatcac ggtgatgttt
     1021 ttgagacgcg tcctcctcgc ggcgccgcgc ggtttctgtg ctggcgtcgt ccgggccatc
     1081 gatgtcgtca acctggcgct cgagtgcgtg gacgctcctc tctacgtccg gcgcgaaatc
     1141 gtgcacaacc tccacgtcgt gcggggcttc gagaagagag gcgtccggtt cgtggaggag
     1201 ctcgacgaag tgcccgacgg cgcgaccgtc gtcttttccg cgcacggcat tgccccgcgc
     1261 gtgcgcgatg aggcgcggcg gcgcgggctg aacgtgatcg acgcgacctg tcccctcgtg
     1321 accaaggtgc acctcgaagc gatccgcttc gagcggaggg gcttccgcat catcctgatc
     1381 ggccatccca accacgagga ggtcgagggg acgatgggcg aggccgagtc ccgaatgacg
     1441 ctggtctcct cgatcgcgga agcggagtcc gtcgaggtcg aggatccgga tcgggtcgcc
     1501 tacatcacgc agacgacgct ttcgatggag gacacgcgcc ccatcgtcga aaccctgaag
     1561 cggcgctttc cggggatcgt cggcccggcg agggacgaca tctgctacgc cacgcagaac
     1621 cgccagatcg ccgttcgcgc gctcgcgcgg gaggcgcccg tcatcctcgt gatcgggtcg
     1681 aagaactcct cgaactcgaa ccgcctcgtc gaggaagcgc agctcgccgg ggctcgggcg
     1741 tatctcgtcg acgacgcttc cggcgtcgat ccggcgtggc tcgaaggcgt ggagaccgtc
     1801 ggcgtgacgt cgggcgcgtc ggctccggag ttcctcgtcg agcagatggt cgcgcacctg
     1861 acggcgctcg gcgcagagcg cgtggacgac ctcgtcacgg tcgaagagga cgtgcacttt
     1921 ccgctccccg gcgagctcct cgaggcccgg cagcggaggg gcacagtggc gctaccgacg
     1981 atttgattcg acgcctcgac ggaaaggtcg cgctcgtcac cggcgcgtcc cgcgggatcg
     2041 gcgaagcgat cgtgcggcgc ttcggcgccg atggagcgcg agtggcgctc gcagcgcgcg
     2101 acgtgcaggc ctgcgagcg
//
LOCUS       DASWAV010000420         4136 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U2665
            k141_782048, whole genome shotgun sequence.
ACCESSION   DASWAV010000420 DASWAV010000000
VERSION     DASWAV010000420.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960316
            Sequence Read Archive: SRR10260007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 4136)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4136)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:47:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,929
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4136
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_782048"
                     /isolate="SMAG_U2665"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.36444 N 132.31889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..431)
                     /locus_tag="VF958_08715"
     CDS             complement(<1..431)
                     /locus_tag="VF958_08715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9149227.1"
                     /translation="MLPVWGEAVLALVAVAAAVLAWRARSRGRILSDRLAARERRGHF
                     LGESPEPAAILRHAYAAAREIVPVTRFDLYRIAPDGGVEEAWTLQRAESGEFEPALHL
                     GSPFAGRKVDARRLRDLSVTETERSFAPKDLLHGGRPRRLRL"
     gene            complement(507..743)
                     /locus_tag="VF958_08720"
     CDS             complement(507..743)
                     /locus_tag="VF958_08720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9149228.1"
                     /translation="MKKFLTLVLAVAFGTVLLTGCKKKEETTTTTETTTTEAPAVVQE
                     TSTTMTTATTMTETPAMTATTETTTTTTTTTVTP"
     gene            978..2315
                     /gene="ffh"
                     /locus_tag="VF958_08725"
     CDS             978..2315
                     /gene="ffh"
                     /locus_tag="VF958_08725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006521882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0048501 - signal recognition particle,
                     plasma membrane targeting [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005048 - signal sequence binding
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HEX9149229.1"
                     /translation="MGERIQGVFKELRGEGHLTEYHLDSALRQIRLSLLEADVALPVV
                     KDFIARVKERAVGAKVMQQLSPAQEVTRIVRDELVGLLGGQKSEISLDGRPAVIEMVG
                     LQGSGKTTSAAKLALHLKGRGRYPLLVAADLSRPAAVSQLKTLGRQIGVPVFDPGELR
                     DAVGVAREGVTEARLTGRDTVIVDTAGRLHIDEELMNEVRAVTEAVRPNEVLFVADAM
                     TGQDAVRSAAGFGAVLPLTGIVFTKSDGDARGGAALSVVSTVGKPIKFVGTGEKPEDF
                     EPFHPDRMASRILGLGDVLTLIEKVEAQVDASAAEKMARRATSGEFTLEDLAEQLRSM
                     KKMGPVSGLLDLLPKGGAFRGLAQPDAVDDRSLVRISAIIDSMTPDERRYPQLLNGSR
                     KKRIASGSGTAVSEVNQLLKQYQQMRKLMKTAKKGARGLDLSRLPLPAPRNSH"
     gene            2376..2627
                     /gene="rpsP"
                     /locus_tag="VF958_08730"
     CDS             2376..2627
                     /gene="rpsP"
                     /locus_tag="VF958_08730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009324393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HEX9149230.1"
                     /translation="MLRIRLRRMGARNRPHYRIVVSDSRSTARAEVLEELGAYDPMRE
                     PPIVSFDRERARHWIGKGATVTPTVRSLLEKKEPEAKAS"
     gene            2674..2904
                     /locus_tag="VF958_08735"
     CDS             2674..2904
                     /locus_tag="VF958_08735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013145774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HEX9149231.1"
                     /translation="MRRLIETIVRRLSDRPEDTRVSETVEGHALVYEISVPEGERGRL
                     IGRDGRTVRAIRAFVGAAAAARGKRVTVRIRD"
     gene            2928..3416
                     /gene="rimM"
                     /locus_tag="VF958_08740"
     CDS             2928..3416
                     /gene="rimM"
                     /locus_tag="VF958_08740"
                     /inference="COORDINATES: protein motif:HMM:TIGR02273.1"
                     /note="Essential for efficient processing of 16S rRNA;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HEX9149232.1"
                     /translation="MVRKPHGVSGEVAVEPVTDFPDRFVEGLRLFWRSADSERELIVV
                     SARPSGRRLLIQFDGVEDAASARELAGGELSVPEGEAVEAPEGFYYSHRIEGWRCEDT
                     QGRSLGRVVKLEHTAGGPMLSLAAGGREAVSIPFTRPIVVSVEPDEQRIVVDPPEGLM
                     DL"
     gene            3440..>4136
                     /gene="trmD"
                     /locus_tag="VF958_08745"
     CDS             3440..>4136
                     /gene="trmD"
                     /locus_tag="VF958_08745"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013013294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052906 - tRNA
                     (guanine(37)-N1)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HEX9149233.1"
                     /translation="MQIEILTIFPGYFRSALAESLLGKAIEAGRLEVTITDLRSFAEG
                     RHRPVDDAPYGGGAGMVMMAPVVVAAVEARRDAPGLPHAWTVLLTPDGERLHQALVES
                     LAARERLLLFCGRYEGLDERARLLGLFDAEISLGDFVLPGGESAALALTEAVSRLVPG
                     VVGSAESLEEESFSRERLDYPHYTRPREFRGLAVPETLLSGDHAKIRRWRRARALERT
                     RERRPDLLERRPEE"
BASE COUNT          642 a         1350 c         1459 g          685 t
ORIGIN      
        1 aggcgcagcc gccggggacg acctccgtgg agcagatcct ttggagcgaa cgatcgttcg
       61 gtctccgtta cggagaggtc gcggagtcgg cgcgcatcca ccttgcggcc cgcgaacggg
      121 ctcccgagat ggagcgcggg ctcgaactcc ccgctctccg cccgctgcag tgtccaggcc
      181 tcttcgaccc ctccgtccgg agcgatccgg tagaggtcga aacgcgtgac cggcacgatc
      241 tcgcgagcag ccgcgtatgc gtgcctcagg atcgccgccg gctcgggaga ctcgccgagg
      301 aagtggccgc ggcgctcgcg ggcggcgagg cgatcggaga ggatccgacc ccgcgagcgg
      361 gcgcgccagg cgagcactgc cgccgcgacc gcaacgagcg cgagaacagc ctctccccag
      421 accggcaaca ccgatttcaa gtgtagccgg accaaaaacg aaaggccccc gatttcgggg
      481 gcctcgcgtg acaagaaaga gaagagttac ggggtgaccg tcgtggtcgt cgtggtcgtc
      541 gtggtttccg tcgtggcggt catcgccggg gtctccgtca tggtggtcgc ggtggtcatg
      601 gtggtcgagg tttcctggac gacagccgga gcctcggttg tggtggtctc cgtggtcgtc
      661 gtggtctcct ccttcttctt gcagcccgtc aggagcacgg tcccgaacgc gactgcgagc
      721 accagggtca ggaacttctt catctccctc tctccttctc gtttatggcg aactttggtt
      781 gcggagcgcc tcggaatatg tgggaaaaaa gcgagcgcat gataccgcaa cagggggggc
      841 tgtcaagcgg gagaagcccg gcccgccgtg cgtttgccat tcttactgga gtgggtggag
      901 gtccgcagga cctgtccccg cgacgttgtt ggggcgtatc cttgcgggct ttcgcgggag
      961 gcgagttgtt cgaatcgttg ggagagcgaa tccagggcgt cttcaaggag ctccgggggg
     1021 aggggcacct gacggagtac cacctcgatt ccgccctccg ccagattcgt ctctccctgc
     1081 tcgaagccga cgtcgcgctc ccggtcgtca aggatttcat cgcgcgggtg aaggagaggg
     1141 ccgtgggcgc gaaggtgatg cagcagctct ctccggcgca ggaggtcacg cgcatcgtgc
     1201 gtgacgagct cgtcggcctg ctcggcgggc agaagtcgga gatctcgctc gacgggcggc
     1261 cggcggtcat cgagatggtg ggcctccagg ggtcggggaa gacgacgtcg gccgcgaagc
     1321 tcgcgcttca cctcaagggg cgtggccgct acccgcttct cgtggcggca gacctctccc
     1381 gtccggcggc cgtctcgcag ctgaagacgc tcgggcgaca gatcggcgtc ccggtattcg
     1441 atccggggga gcttcgcgat gccgtcgggg tggcgcgcga gggcgtgacc gaggcgcggc
     1501 tgaccggccg agataccgtc atcgttgata ccgccggccg gcttcacatc gacgaagagc
     1561 tgatgaacga agtccgtgcg gtgaccgagg cggtgcggcc gaacgaggtc ctcttcgtcg
     1621 cggacgcgat gacggggcag gacgcggtgc gctccgccgc cggattcggc gcggtgctgc
     1681 ctctgaccgg aatcgtcttc acgaaatcgg acggcgacgc ccgtggcgga gcggcgctct
     1741 cggtcgtctc caccgtcggc aagccgatca agttcgtcgg gacgggggag aagccggaag
     1801 acttcgagcc ctttcacccg gatcgcatgg catcgcggat cctcggcctg ggagacgtcc
     1861 tcacgctcat cgagaaagtc gaagctcagg tggatgcgtc ggccgccgag aagatggcgc
     1921 ggcgcgcgac gagtggcgag ttcaccctgg aggacctcgc cgagcagctg cgctcgatga
     1981 agaagatggg acccgtctcc gggctgctcg acctgctccc gaagggcggc gccttccgcg
     2041 gcctggcgca gcccgatgcc gtcgacgaca ggtcgctcgt ccggatctcc gcgatcatcg
     2101 actcgatgac gcccgacgag cgcaggtatc cccagttgtt gaacggtagc cgcaagaaac
     2161 gcatcgcatc cggttccggc acggccgtct ccgaggtcaa ccagctcctg aagcagtacc
     2221 agcagatgcg caagctcatg aaaaccgcga agaagggggc ccgcgggctg gacctttcgc
     2281 ggctgccgct ccccgcgccc cgcaactcgc actagaatgg gtggcccgtt tgtggcaagt
     2341 ggagacctga ccccgccgga aaggaaaagt cgatcatgct gagaattcga ctgcgccgca
     2401 tgggcgcacg caatcgcccg cattaccgca tcgtcgtctc ggactcgcgc tcgaccgcgc
     2461 gggccgaagt actcgaggag ctcggcgcct acgacccgat gcgcgagcct ccgatcgtct
     2521 ccttcgaccg tgagcgcgcg cgccactgga tcggcaaggg cgcgaccgtg acgcccacgg
     2581 tccgctcgct gctcgagaag aaggagccgg aggcgaaggc gtcgtgaccg gcttctcctc
     2641 gtctcccgtc ttccgtcttc cgtctcccgc gtcatgcgcc ggctgatcga gacgatcgtt
     2701 cgacggctct ccgaccgtcc cgaggacact cgggtgagcg agacggtcga gggccacgcg
     2761 ctcgtgtacg agatctccgt gcccgaaggg gagcgcggcc ggctcatcgg ccgggacggc
     2821 cgcaccgtcc gggcgatccg cgctttcgtg ggcgcggccg ccgcggctcg cggaaaacgg
     2881 gtcacggtgc ggattcgcga ctgacggagt cgcgccttct cgtcggaatc gttcgaaagc
     2941 cacacggcgt ctccggcgag gtcgcggtcg agcccgtgac ggactttcca gatcgattcg
     3001 tcgaaggcct tcggctcttc tggcggagcg cggattccga gcgggagctc atcgtcgtgt
     3061 cggcccggcc gagcgggcgg cgcctcctga tccaattcga tggcgtcgaa gacgcggcgt
     3121 ccgcgcgcga gctggcgggc ggtgaactct ccgttccgga gggggaggcg gtcgaggcgc
     3181 cggagggctt ctactacagc catcggatcg aaggttggcg atgcgaggac acgcaggggc
     3241 gctcgctcgg ccgcgtcgtg aaactcgagc acaccgccgg aggtccgatg ctctcgctcg
     3301 cggcgggcgg ccgggaagcg gtatcgattc cgttcacgcg gcccatcgtg gtatccgtcg
     3361 agcccgacga gcagcggatc gtcgtcgatc cgcccgaagg cctgatggac ctgtgactcg
     3421 aacgtggacg ttgaaatcga tgcagatcga aatcctgacg atctttcccg gttattttcg
     3481 gtcggcgctc gccgagagcc tcctcggcaa ggcgatcgaa gccggacggc tggaggtgac
     3541 gatcaccgat ctgaggtcct ttgcggaagg tcgccatcgc ccggtggacg acgcgcccta
     3601 cggcggggga gcggggatgg tcatgatggc gccggtcgtc gtcgccgccg tcgaggcccg
     3661 gcgcgacgcc cccggacttc cccacgcctg gaccgtgctg ctcacgcccg acggagagag
     3721 actccatcag gcgctcgtcg aatcgctcgc cgcccgcgag cggttgctcc tcttctgcgg
     3781 ccggtacgaa gggttggacg agcgggctcg gcttctcggc ctcttcgatg ccgagatttc
     3841 gctcggagac ttcgtgctgc ccggcggaga gtccgccgcg ctcgcgctga cggaggcggt
     3901 gtcccgtctc gtgccgggcg tcgtgggatc cgcggagtct ctcgaggagg agtctttctc
     3961 gcgcgagcgc ctcgactacc cgcactatac gaggccgcgc gagtttcgag gcctggccgt
     4021 cccggagacg ctcctctccg gcgaccatgc gaagatccgc cgctggcgcc gcgcccgcgc
     4081 cctcgaacgg acccgtgagc gcagaccgga cctcctcgag cgccggccgg aggagc
//
LOCUS       DASWAV010000421         6470 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U2665
            k141_504105, whole genome shotgun sequence.
ACCESSION   DASWAV010000421 DASWAV010000000
VERSION     DASWAV010000421.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960316
            Sequence Read Archive: SRR10260007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 6470)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6470)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:47:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,929
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6470
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_504105"
                     /isolate="SMAG_U2665"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.36444 N 132.31889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1432)
                     /locus_tag="VF958_08750"
     CDS             complement(<1..1432)
                     /locus_tag="VF958_08750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017668830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HEX9149234.1"
                     /translation="MNRRTFLEALAAGAATFALPAPGRPQTPDLAPVFAEVEKRHGEA
                     VRRLQEWIRQPSIAAENRGVEEGNALLIRMLRDAGFDTAVRVPTDGQPGAFATLDAGA
                     KRTVGVYFMYDVKQVDPAEWSSPPWDAAIVEKSGFGKAIVGRGAVNQKGPEAAFLAAL
                     HAFRGAGRKLPVNLVLVAEGEEEIGSPHFPQIVRRPEVQAALSKTTGVFMPSASQGAD
                     GVVQVSLGAKGVVELELVSTGGKWGRGPEKDIHSSNKARVDSPAWHLVQALNTLVTAE
                     GEPAIDGFADRARPISAAEKAMVAEGARRFDEAILKKQMGVRHWIKDVGFVESLERLV
                     SQPTVNIEGLVGGYTGPGGKTILPYKAVAKLDLRLVPDMKAADALAALKGHLAKRGFG
                     DIEANMTGGYDPTDTAANAPLIRAQVSVLRRDGIDPLMWPRNAGSWPGYVFTGEPLSL
                     AAGHFGLGHGSGAHAPDEYYVIESANP"
     gene            complement(1439..3163)
                     /gene="mutL"
                     /locus_tag="VF958_08755"
     CDS             complement(1439..3163)
                     /gene="mutL"
                     /locus_tag="VF958_08755"
                     /inference="COORDINATES: protein motif:HMM:TIGR00585.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HEX9149235.1"
                     /translation="MPRIHRLTSEVIDRIAAGEVVERPASVVKELVENALDAGATRVD
                     VEVDGGGIARILVRDDGSGMSAEDARLSLERHATSKISSDGDLAGVASFGFRGEALPS
                     IASVSKLTLTTSDGSSPEAVRLTVDYGRAAAEEPAARPRGTDVLVENLFARTPARRKF
                     LASPEAEGRAAAAAVTRAALSHPAVAFSLRSRGRETLDAPSAVDRAARILQLFGRETL
                     GELEVFEARAGDLKVVGYATRGSVTFPNRRYQYLFVNGRPVEDRALSRAILQASRDAI
                     RTDRHPAVFLYLSAKAGAVDVNVSPAKTQVKFADAGTAFRLVYHAVQSALLAGKEERR
                     LKSVPSEGRLAEPRETYVAEGVPTPAPGSPVVLPPVPKPETPPPPVEKKTPELVAIGQ
                     YRESFVLASGPEGLLVIDQHAAHERILYERIRDRIASGRILAQRLLLRVLFEATPEEA
                     ETLAARYEDLSAAGFEIEPMSGRSYAIAALPAEATDRDPAETLREALDGLAETGDWDA
                     ERRRDRLAATIACRSAVTIRYHLAPEEIRRLLSDWMKTADRFTCPHGRPVVLLTTDED
                     LEKYFKRR"
     gene            complement(3245..4894)
                     /locus_tag="VF958_08760"
     CDS             complement(3245..4894)
                     /locus_tag="VF958_08760"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007288018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HEX9149236.1"
                     /translation="MSLLFLFAVTLAPTAPPADLILVSARIWTGDPSRPEASALAARN
                     GRIMAIGGDAEIAALKGSKTVVVDGKGRRVVPGFIDCHTHMSMGGFNRLAVDLRHTKD
                     PADFTARLAEFARTRPPGLWLTDGAWDHQVWGNPILPTRAMLDPATGDRPTCLSRTDG
                     HMVVCNSRALALAKITRESPDPPGGIIVRDAAGEPTGVLKDAAMDLIGAVRPARTRAE
                     VEEALATAVRHAAENGVTSVQDLPGNALDLDAWENLRAAGKLTVRVNYRPSLSEWEKA
                     RDRRKTMKNDEWLRVGGVKGYMDGSLGSGTALFFQPYADDPKGTGVYAAEAIPLSRLE
                     TRVAAADAAGLQVEIHAIGDRANAELLGIFERVAKKNGARDRRFRIEHAQHLRAEDIP
                     RFARLGVIASMQPYHAVDDGRWAEKRIGIDRCRTTYAFRSLEKTGATLVFGSDWDVAP
                     LSPLAGIDAAVNRRTLDGKHPDGWIPAERIAVVDALRAYTSSAAWAAFEEGEKGTLGD
                     GKLADFVVLSRDPLSIPKRELDSVRVDTTVAGGRVIFSAGR"
     gene            complement(4891..>6470)
                     /locus_tag="VF958_08765"
     CDS             complement(4891..>6470)
                     /locus_tag="VF958_08765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014559212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="putative PEP-binding protein"
                     /protein_id="HEX9149237.1"
                     /translation="LLAPIFPSKEKEAAIKGGRLLAKGLPAGPGAACGRVAFTADRAV
                     EMAAKGDPVVLVRVETSPEDIAGMHAAVGILTTRGGMTSHASVVARGMGKTCVVGAGE
                     ITVDETKGELRARNLSAKEGDFLSVDGTAGEVILGKLPTQPSEILQVLIEGSLAPEKA
                     PLFGAFTRLLEWADRARRLGVRANADTPTDARVARLFGAQGIGLCRTEHMFFGENRIV
                     AVRQMIVAETQAERRAALAKLLPMQREDFVGIFREMGERPVTIRLLDPPLHEFLPHEE
                     DAVQETAKELGISAEKLRERVRALAEANPMLGHRGCRLGITFPEIYEMQVQAIFEAAA
                     EARRQGQRPNPEVMIPLVGTKGEFDRLRALTDEVARRVASETGIDVSYRVGTMIEIPR
                     AALRAAEIAENCDFFSFGTNDLTQMTYGYSRDDVASFLPAYIEAGILPDDPFSTIDQE
                     GVGELVSVGTKRGRETRHDLKVGVCGEHGGDPRSIHFFHGVGLDYVSCSPFRVAVARL
                     AAAHAALSGGGGISSTA"
BASE COUNT          939 a         2287 c         2214 g         1030 t
ORIGIN      
        1 tcgggttcgc cgactcgatc acgtaatact cgtcgggcgc gtgcgcgccg ctcccgtggc
       61 cgaggccgaa gtggccggct gcgagcgaga gcggctcgcc cgtgaaaacg tacccgggcc
      121 aggagcccgc gttgcgcggc cacatgaggg ggtcgatccc gtcgcgtcgg agcaccgaga
      181 cctgcgcgcg gatcaaaggc gcgttcgcgg cggtgtccgt cggatcgtag ccgcccgtca
      241 tgttggcttc gatgtcgccg aagccgcgct tcgcgagatg gcccttcaat gcggcgagcg
      301 cgtccgccgc tttcatgtcg gggacgagcc gcaggtcgag cttcgcgacg gccttgtacg
      361 gaagaattgt cttgcctccc gggccggtgt atccgccgac gagcccctcg atgttgaccg
      421 tcggttgcga caccagccgc tcgagcgatt cgacgaaccc gacgtccttg atccagtgac
      481 ggacccccat ctgctttttc aggatcgctt cgtcgaaacg ccgcgcaccc tcggcgacca
      541 tcgccttctc cgccgccgag atcggccggg cgcgatccgc gaagccgtcg atcgcgggct
      601 cgccctccgc ggtgacgagc gtgttgagcg cctggacgag gtgccatgcc gggctgtcca
      661 cccgcgcctt gttgctcgag tggatgtcct tctcgggtcc gcgcccccac tttccgccgg
      721 tcgagacgag ctcgagttcg acgacgccct tggcgccgag cgacacctgg accacgccgt
      781 ccgcgccctg cgacgcggac ggcatgaaga ctcccgtcgt tttcgagagc gccgcctgca
      841 cctcgggccg ccggacgatc tgggggaagt gcggcgagcc gatctcctcc tcaccttccg
      901 cgacgaggac gaggttcacc ggcagcttcc gaccggcgcc gcggaacgca tgcagcgccg
      961 cgaggaacgc ggcctccggc cctttctggt tgacggcgcc gcggcccacg atcgcctttc
     1021 cgaagccgga cttctcgacg atcgcggcgt cccacggcgg agaggaccac tcggcggggt
     1081 cgacctgctt gacgtcgtac atgaaataga cgccgacggt ccgcttcgcg ccggcgtcga
     1141 gcgtcgcgaa ggcccccggc tggccatccg tgggaacgcg cacggcagtg tcgaacccgg
     1201 cgtcgcgcag catccggatg agaagcgcgt tgccctcctc caccccgcgg ttctcggcgg
     1261 cgatcgacgg ctggcggatc cactcctgta gccggcgcac cgcctcgccg tggcgcttct
     1321 ccacctccgc gaaaacgggc gcgagatccg gggtctgcgg tcgtcccggc gccggaagcg
     1381 cgaacgtcgc ggcgcccgcg gcgagcgctt ccagaaaggt tctcctgttc atcgcggtct
     1441 acctcctctt gaagtatttc tcgaggtctt cgtcggtcgt cagcagcacg accggacggc
     1501 cgtgcgggca cgtgaaccgg tcggcggtct tcatccagtc ggaaagaagg cggcggatct
     1561 cttcgggcgc gaggtggtag cgaatcgtaa cggcggaacg gcaggcgatc gtcgccgcca
     1621 gccgatcgcg gcgccgctcc gcatcccagt cccccgtttc cgccagaccg tcgagggctt
     1681 cgcgcagcgt ctcggcgggg tcgcgatccg tcgcctcggc cggcagagcc gcgatcgcgt
     1741 acgaacggcc ggacatcggc tcgatctcga accccgccgc ggagaggtcc tcgtatcgcg
     1801 ccgcgagcgt ttccgcctct tcgggagtcg cttcgaagag gacgcgcagg agcagccgct
     1861 gcgcgaggat ccggcccgag gcgatgcggt cgcggatccg ctcgtagagg attcgctcgt
     1921 gcgccgcgtg ctgatcgatg acgaggagtc cttcgggtcc ggaggcgagc acgaacgact
     1981 cccggtactg cccgatcgcg acgagctcgg gcgtcttctt ctcgacgggc ggcggcgggg
     2041 tctcgggctt cgggacgggc ggcagcacca cggggctgcc gggagcgggc gtcgggactc
     2101 cctccgcgac gtaagtttcc cggggttccg ccaggcgccc ttcactcggc acggatttca
     2161 gtcggcgctc ttccttgccg gcgaggagcg ccgactggac cgcgtggtag acgaggcgga
     2221 acgccgttcc cgcgtcggcg aacttcacct gggtctttgc cggtgaaacg ttgacgtcga
     2281 ccgcgcccgc cttcgcgctc aggtagagga aaaccgccgg atgccggtcg gtgcggatgg
     2341 cgtcccgcga cgcctggagg atcgcgcgcg agagggcccg gtcctcgacc ggccggccgt
     2401 tcacgaacag gtactgataa cgccggttgg gaaacgtcac ggatccgcgc gtggcgtacc
     2461 cgacgacttt caggtctccg gcgcgcgcct cgaagacctc cagctcgccg agcgtctccc
     2521 ggccgaagag ctggagaatg cgggcggcgc gatcgaccgc cgacggagcg tcgagagtct
     2581 cgcggccgcg cgaacggagc gagaacgcaa cggccgggtg cgacagggcc gcgcgcgtga
     2641 ccgccgccgc cgcggcgcgc ccctccgcct ccggcgaggc gaggaacttc cggcgcgctg
     2701 gcgtgcgcgc gaagaggttc tcgacgagta cgtcggttcc ccgcgggcgc gccgcgggct
     2761 cttcggccgc ggctcgcccg tagtcgacgg tgagccgcac cgcctccgga gacgatccgt
     2821 ccgaggtggt gagcgtcagc ttcgacaccg aggcgatgga cgggagcgcc tccccgcgga
     2881 agccgaaact cgcgacgccc gcgagatcgc cgtccgacga gatcttcgac gtcgcgtggc
     2941 gctcgagcga gagccgagcg tcctcggccg acattccgga gccgtcgtcg cggacgagga
     3001 tccgtgcgat cccgccgccg tccacctcga cgtcgacgcg cgtcgcgccg gcgtcgagcg
     3061 cattttcgac gagctccttg acgaccgacg ccggccgctc gacgacctct cccgcggcaa
     3121 tgcggtcgat cacctcgctc gtgaggcggt ggatcctggg cacgtcgtgg gagtttatcg
     3181 gctctcactc cctctcccac cgggagagtg gcggggtgag ggggcaccga gtcgccgcga
     3241 gatttcagcg cccggcgctg aatatcaccc ggccgccagc gacggtcgtg tccaccctca
     3301 ccgagtcgag ctcgcgcttc ggaatcgaca ggggatcgcg cgacaggacg acgaaatccg
     3361 cgagcttccc gtcaccgagc gtccccttct ccccctcttc gaacgctgcc caggcggcgc
     3421 tcgacgtgta ggcgcgcagc gcgtcgacga ccgcgatcct ttctgcggga atccacccat
     3481 ccgggtgctt tccgtcgagc gtccggcggt tgacggcggc gtcgattccg gcgagcgggg
     3541 agagcggcgc gacgtcccag tccgagccga agacgagcgt tgcgccggtc ttctcgagag
     3601 accggaaggc ataggtcgtc cggcaccggt cgataccgat ccgtttctcc gcccaccggc
     3661 cgtcgtcgac cgcgtggtac ggctgcatcg aggcgatgac gccgaggcgc gcgaaccgag
     3721 ggatgtcctc ggcgcggagg tgctgcgcgt gctcgatgcg gaaccgccgg tccctcgcgc
     3781 cgttcttctt cgcgacccgc tcgaagattc cgaggagctc cgcgttcgcg cggtcgccga
     3841 tcgcgtgaat ctcgacctgc aggcctgcgg cgtccgccgc ggcgacgcgc gtctcgagcc
     3901 tcgagagcgg gatcgcttcc gccgcataga cgccggtccc cttcgggtca tcggcgtagg
     3961 gctggaagaa cagagccgtc cccgacccga gcgagccgtc catgtaaccc ttcactccgc
     4021 cgacgcgaag ccactcgtcg ttcttcatgg tctttcggcg gtcgcgagcc ttctcccact
     4081 cggacagcga gggccggtag ttcacgcgga ccgtgagctt gcccgctgcg cgcaagttct
     4141 cccatgcgtc gaggtcgagg gcgttgcccg gaagatcctg aacggacgtc actccgttct
     4201 cggcggcatg gcgcacggcg gtggcaagcg cctcttcgac ctccgcccgg gtgcgcgccg
     4261 gacgcaccgc gccgatcaga tccatggcgg cgtccttgag gacgcccgtc ggctcgcccg
     4321 ccgcgtcgcg gacgatgatt ccacccggcg gatccgggct ttcccgggtg atcttcgcga
     4381 gcgccagagc gcggctgttg cagaccacca tgtggccgtc ggtccgcgac agacaggtcg
     4441 ggcggtcgcc ggtcgccggg tcgagcatgg cgcgcgtcgg caggatcgga ttcccccaca
     4501 cctggtggtc ccaggcgcca tcggtgagcc acaaccccgg cggtcgagtc ctcgcgaact
     4561 cggcgaggcg tgccgtgaag tcggcaggat ctttcgtgtg gcgcaggtcg accgcgagcc
     4621 ggttgaaccc tcccatcgac atgtgcgtgt ggcagtcgat gaatccgggg acgacgcgtc
     4681 gccccttccc gtcgacgacc acggtcttcg atcccttgag cgccgcgatc tccgcatcgc
     4741 cgccgatcgc catgatccgg ccgttgcgcg ccgcgagggc cgaagcctcc ggccgcgagg
     4801 ggtcgcccgt ccagattctt gcggagacga ggatcaggtc ggcaggcgga gcggtgggcg
     4861 ccagagtcac tgcgaagagg aagaggaggc tcacgcggtc gacgagatgc cgccgccgcc
     4921 ggagagggcc gcatgcgccg ccgcgaggcg cgcgacggcg acccggaacg gcgagcacga
     4981 gacgtagtcg aggcccacgc cgtggaagaa gtgaatcgag cgcgggtccc cgccgtgctc
     5041 cccgcagacg cccactttga ggtcgtgccg cgtttcgcgg ccgcgtttcg ttccgaccga
     5101 cacgagctcg ccgacaccct cctggtcgat cgtcgagaac gggtcgtccg ggaggattcc
     5161 ggcctcgatg tatgcgggca ggaacgacgc gacgtcgtcg cgggagtagc cgtaggtcat
     5221 ctgcgtcagg tcgttggtac cgaacgagaa gaaatcgcag ttctcggcga tctccgccgc
     5281 ccgcaaagct gcgcgcggta tctcgatcat cgtgccgacg cggtaagaca cgtcgatgcc
     5341 cgtctccgac gccacgcgac gcgcgacctc gtcggtgagc gcgcggagcc ggtcgaactc
     5401 ccccttcgtt ccgacgagag ggatcatcac ctcggggttc ggacgctggc cctggcgacg
     5461 cgcctccgcc gccgcctcga agatcgcctg cacctgcatc tcgtagatct ccgggaacgt
     5521 gatgccgagc cggcagcccc ggtgaccgag catcggattc gcctccgcga gagcccgaac
     5581 tcgttcgcgg agtttttcgg cggagatgcc gagctccttc gccgtctcct gcaccgcgtc
     5641 ctcttcgtgc ggcaggaact cgtggagcgg cggatccagc aggcggatcg tgacgggccg
     5701 ctcccccatc tcgcggaaga tcccgacgaa gtcctcgcgc tgcatgggaa ggagcttcgc
     5761 gagagcggcg cggcgctcgg cttgcgtttc cgcgacaatc atctggcgca cggccacgat
     5821 gcggttctca ccgaagaaca tgtgttcggt tcgacagagg ccgatcccct gcgcaccgaa
     5881 caaccgggcg acccgcgcgt cggtgggagt atcggcgttt gcacgcacgc cgagtcttcg
     5941 cgcgcgatcg gcccactcga gcaggcgcgt gaacgcgccg aagagcggcg ccttctccgg
     6001 cgcgagagac ccctcgatca gtacctggag gatctccgac ggctgtgtcg ggagcttccc
     6061 gagaatgacc tctcctgccg tcccgtcgac ggacaggaag tcgccctctt tcgccgacag
     6121 gtttcgcgcg cgaagctcgc ccttcgtctc gtcgacggtg atctcgcccg cgccgacgac
     6181 gcaggttttg cccattccgc gcgcgacgac ggaggcgtgc gaggtcattc cccctctcgt
     6241 cgtcaggatc ccgaccgcgg catgcatgcc cgcgatgtcc tcgggagacg tttcgacccg
     6301 cacgagcacg accggatcgc ctttcgccgc catctccacc gcacggtccg cggtgaacgc
     6361 gacccgtccg caggccgctc ccggtccggc gggcagtccc ttcgcgagaa ggcgcccccc
     6421 cttgatcgcc gcttccttct ctttcgaagg aaatatgggg gcgagcagct
//
LOCUS       DASWAV010000422         3247 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U2665
            k141_342390, whole genome shotgun sequence.
ACCESSION   DASWAV010000422 DASWAV010000000
VERSION     DASWAV010000422.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960316
            Sequence Read Archive: SRR10260007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 3247)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3247)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:47:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,929
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3247
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_342390"
                     /isolate="SMAG_U2665"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.36444 N 132.31889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            678..1796
                     /locus_tag="VF958_08770"
     CDS             678..1796
                     /locus_tag="VF958_08770"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HEX9149238.1"
                     /translation="MLRLAARRILALALVLAGSGAARADVESVYDGGSGAGSRRRTIE
                     RARRLLADVLELYPGSTVAVALDDQIVWSAGFGYANARRQIPVTSQTQFRVDRVSEAL
                     TATALLRLAEEGKIDLDAPVQRYVPAFPEKAYPITPRQLAAHLAGIPALPPERWNASL
                     HCAQPEEAFRSFAALPLSRIPGSRFLHSMPGYVLLSAVVANACGQDFVSCLAEKVFRP
                     AGMTSTVLDDPRSPAPRLSASYERGWFGMLAAAPAADNTCKWGAGGFVSTAEDLARFG
                     MALLRGELLRRESTALLFSPQRTSSGESTGYGLGWQVGVDAGGRRRLVQSGRTAGGRS
                     VLVLVPEARLTVAVLANVNGEHLDDHALKIAELFAEPD"
     gene            1943..2227
                     /locus_tag="VF958_08775"
     CDS             1943..2227
                     /locus_tag="VF958_08775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9149239.1"
                     /translation="MQSSAKQWTRTLAAGLMSSVLFAGAAFGAEQRMTAKKSTASRRA
                     ATEGPRVVGFKEERTDSWLCEYVSPFFCSSIPTAVTAPQPGTTTTSRGRR"
     gene            2352..>3247
                     /locus_tag="VF958_08780"
     CDS             2352..>3247
                     /locus_tag="VF958_08780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HEX9149240.1"
                     /translation="MSDGALKPNVDSDGLAAALRKVVRGEVRFDAGSRALYASDSSNY
                     RQVPIGVVVPRDRDDVLETLARCREFGAPILGRGAGTSLAGQCCNVAVVLDTSKYLNR
                     ILEIDPVARTARVEPGVILDDLRDAAEQHGLTFGPDPATHACCTLGGMIGNNSCGVHS
                     VMAGKTDDNVLDLEVVAGDGARMRVGQTSEADFVRIVSEGGRRAEIYAKLRDLADRHG
                     PLIRSRYPPIPRRVSGYNLPYLLPENGFDLARALVGSEGTCAFLLEATVRLVWSPPAR
                     VLVVLGYPDVFAAADAVPEILT"
BASE COUNT          511 a         1132 c         1067 g          537 t
ORIGIN      
        1 tgatcgttcc gaagagtgtc ccggtggtgg cggcgaaggc gcccgccgcg atgccaagag
       61 cgaccagaac gagcgccgca agcttcgtaa atcgattctt catggctgtt cctttcatcc
      121 cgtttcctgg tttcggtttt ccgtttcccg atttgcgcag aagagcaaca cggttgccac
      181 gggtcatcga aggcgtcgcg cgacggaacc accgccgccg aggacgaacg agtcgcgcga
      241 atggacggat cgttcggcgc gaaatcgagg ccgcggcgcg ctccagatct ccggacggtt
      301 cctcaaatac ttggatggcg gcgcgaaacg ttgggcccga tccgcgatct ctcccgtgtc
      361 acgcgaaccg gaaaaaggag gacgaagaag cgcggcgtcg ggacaacggt ggaatggctc
      421 ccggcgttcg cgaaggcgtc cgatcggccg gatttcgaga gctttgtgcg cagtcgccgt
      481 cgagcctgcc gcccacgccg aaccgcattc gcgaaaaact ctttcggcgc cgtcctccga
      541 aagcgcgact tcgtcctctc cgcgcgggcc gaccgccgtt cgcggcagcc aacttgctgt
      601 ccccgaagtc cgtggggcga tccccgaaaa ctttttcttc gtccgggccg tttacacacc
      661 gaggaagctc tgcgacgttg ttacggctcg ccgcccggag gatcctcgcg ctggcgctcg
      721 tgctggcggg ctcgggcgcc gcccgggccg acgtcgaatc ggtctacgac ggcggatccg
      781 gggccgggtc ccgacgccgc acgatcgagc gcgcgcgccg gctcctcgcc gacgtcctcg
      841 agctctatcc cggatcgacc gtcgccgtcg cgctcgacga tcagatcgtc tggtccgcgg
      901 ggttcggata cgccaacgcg cggcggcaga tcccggtgac ctcacagacg cagttccgcg
      961 tcgaccgagt ttccgaggcg ctcaccgcga cggcactcct ccggctcgcc gaggagggga
     1021 agatcgacct cgatgcgccg gtccagcgct acgtgccggc gtttccggag aaggcgtacc
     1081 cgatcacgcc gcggcagctc gccgcgcacc tcgccggaat ccccgcactg ccgccggagc
     1141 ggtggaacgc gtcgctccac tgcgcgcagc ccgaggaggc gtttcgatcg ttcgcggctc
     1201 tccccctctc gcgcattccg ggatcgcggt ttctccactc gatgcccggg tacgtcctgc
     1261 tttccgcggt cgtcgcgaac gcgtgcggtc aggacttcgt ctcctgcctc gccgagaagg
     1321 tcttccgccc cgccggcatg acgtccacgg tgctcgacga tccgcgctcg ccggcgccgc
     1381 gcctgtcggc ttcctacgag cgcggatggt tcgggatgct cgccgccgcg ccggccgccg
     1441 acaacacgtg caagtggggc gccggcggat tcgtctcgac cgccgaagac ctcgcgcggt
     1501 tcgggatggc gctcctgagg ggcgagctcc tgcggcgcga gtcgacggcg ctcctctttt
     1561 cgccgcagag gacgtcgagc ggagagagca cgggctatgg actcgggtgg caggtcggcg
     1621 tggacgcagg tggacggcgc cggctcgtgc agagcggacg caccgccggc ggacgctccg
     1681 tcctcgtgct cgtgccggaa gcgcgcttga ccgtcgcggt gctcgcgaac gtcaacggcg
     1741 agcacctgga cgatcacgcg ttgaagatcg cggagctctt cgcggaaccc gattgacggc
     1801 ctttttccgg tccccgctat tgtgacttcg atcaccgact taagtcggtg tacgcgagtc
     1861 acttacacga tggcacacat atcgcaacct ctccctttca gctgcgaagc gaaacgcagc
     1921 cggaaaggaa ggaggtcgcc agatgcaatc gagcgcaaaa cagtggacga ggacgctcgc
     1981 ggccgggctg atgagctcgg ttctcttcgc gggcgccgcc ttcggagcag agcagcgcat
     2041 gacggccaag aagagcactg cgagccgtcg cgcggcgacg gaagggccgc gcgtcgtcgg
     2101 attcaaggaa gaacgcaccg actcctggct gtgcgagtac gtgagtccgt tcttctgctc
     2161 ttcgatcccg acggccgtca cggccccgca gccgggcacg acgacgacga gccgcggccg
     2221 ccgttagagt tccgacgatc cggactcccg cgtgacggga ttctcgatcg acggaaatcc
     2281 gaaaaaagcc ccgcacaaga ggggcttttg attttggggt caggtctcca cttgccacat
     2341 ccggtttccg agtgtcagat ggagccctga aaccgaacgt cgattccgac ggactcgcgg
     2401 cggcgctccg gaaggtcgtc cgcggcgaag tccgcttcga cgcgggcagc cgcgcgctct
     2461 acgcgagcga ctcctcgaac taccgccagg tgccgatcgg agtcgtggtt ccgcgggaca
     2521 gagacgacgt cctcgagaca cttgcccgtt gccgcgaatt cggcgcgcca atcctggggc
     2581 gcggcgcggg aacgagcctc gccgggcagt gctgcaacgt cgccgtcgtc ctcgacacct
     2641 cgaagtacct caaccggatc ctggaaatcg accccgtcgc gcggacggcg cgcgtcgagc
     2701 ccggcgtcat cctggacgac cttcgcgacg ccgccgagca gcacgggctg acgttcgggc
     2761 ccgatcccgc gacgcacgcc tgctgcacgc tgggcggaat gatcggcaac aactcctgcg
     2821 gcgtgcactc cgtgatggcc ggcaagaccg acgacaacgt actcgacctc gaggtcgtcg
     2881 ccggagacgg cgcgcggatg cgggtcgggc agacgtcgga ggccgatttc gtccgcatcg
     2941 tctcggaagg cggccgccgc gccgagatct acgcgaagct ccgcgacctc gccgatcgcc
     3001 atgggccgct catccgctcc cgttatcccc cgattccgcg gcgcgtctcc ggttacaacc
     3061 tgccctacct cctgccggag aacggcttcg acctcgcgcg cgctctcgtc ggctccgaag
     3121 gcacctgcgc gtttctcctc gaagcgacgg tgcggctcgt ctggagcccg ccggcgcgcg
     3181 tcctcgtcgt actcggctac cccgacgtgt tcgctgcggc ggacgccgtc cccgagatcc
     3241 tcaccta
//
LOCUS       DASWAV010000423         2313 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U2665
            k141_103494, whole genome shotgun sequence.
ACCESSION   DASWAV010000423 DASWAV010000000
VERSION     DASWAV010000423.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960316
            Sequence Read Archive: SRR10260007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2313)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2313)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:47:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,929
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2313
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_103494"
                     /isolate="SMAG_U2665"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.36444 N 132.31889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1610)
                     /locus_tag="VF958_08785"
     CDS             complement(<1..1610)
                     /locus_tag="VF958_08785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017571497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2 N-terminal
                     domain-containing protein"
                     /protein_id="HEX9149241.1"
                     /translation="MAKIRVEELAAQMGVGTKEVLFLLQSIGVDVKSPQAALDEATVL
                     AILQGKTHAPKQLIVRDAESKAFRPQKSALSRIKIVEKPLAPAAPSRPEPEPELSPPP
                     PPPPVRPVAAPPAPPVEAKSKPAEPARTAVVPSEPEAEPPPPAKPPLPRKIVLPPPRP
                     AVARPVSPGGPAGMSRAGAPRPTGPGRPMGPASGSPGRPMGPASGSPGRPGPSVYRPA
                     PGGPGMRPAGMPLRPGGPGARPGSLGRPMGPGRAAAPPPATERGPAKRKKEDEKDTKK
                     ATAKRGAKPKITVADEVDLREFVGAYEEDTYSDISLPLIEKGEGAEAEAEASFEPKPV
                     SKSALRRAAKSTRAHDSGKLLEFKKPMPTGPIFLSEGVTVKELSEKLGVLAKDIQRLL
                     MSRGILATVNQTLDAPTAIQIAKEVGVEAAVVSFEEELELARGGAETTPTEAEPGVGA
                     AREPRAPVVTVMGHVDHGKTSLLDAIRQTKVAEGEAGGITQHIGAYRAEVHGRPIVFL
                     DTPGHEAFTSMRARGARATDIVVLVVAA"
     gene            complement(1621..>2313)
                     /locus_tag="VF958_08790"
     CDS             complement(1621..>2313)
                     /locus_tag="VF958_08790"
                     /inference="COORDINATES: protein motif:HMM:NF024582.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HEX9149242.1"
                     /translation="RDVDPVGACVGMRGSRVQSIMRELHGEKIDVILYSDDLITFAQN
                     ALAPAKITRVSPIHHEDTTMSLDCVVEDDQLSLAIGKKGQNVRLASALIGMKIEIKGE
                     TEVKGEVAEALTRMLMASRLKQVAVGDVPGITPKAAERLASAGIGSLSELLEKGVAEL
                     SEIPGIGAATAEKILEAARAAEDEVRNPRPADEEAGAEEESEAAAEDTGEASGEEAGG
                     EPQPAEETIVQE"
BASE COUNT          292 a          811 c          812 g          398 t
ORIGIN      
        1 tccgcggcaa cgacgagcac gacgatgtcc gtggcgcgcg cgccccgggc gcgcatcgaa
       61 gtgaacgcct cgtgacccgg cgtgtcgaga aagacgatcg gccgcccgtg gacctccgcc
      121 cggtaggcgc cgatgtgctg cgtgatcccg ccggcctcgc cctccgcgac cttcgtctgg
      181 cggatcgcgt cgagaagcga cgtcttgccg tgatcgacgt ggcccatcac ggtgacgacg
      241 ggagcgcgcg gctctctcgc cgccccgacg cccggctcgg cctcggtcgg cgtcgtctcc
      301 gcgccgcccc gggcgagctc gagctcctcc tcgaaggaga cgacagccgc ctcgaccccg
      361 acttccttcg cgatctggat cgccgtcggg gcatcgagcg tctgattgac cgtcgccagg
      421 atgccgcggg acatgaggag gcgctggatg tccttcgcga ggaccccgag cttctccgac
      481 agctctttga ccgtgacgcc ctccgacagg aagatgggcc cggtcggcat cggcttcttg
      541 aactcgagga gcttgccgga gtcgtgcgcg cgggtggact tggccgcgcg gcgcagcgcc
      601 gacttcgaga ccggcttcgg ctcgaacgac gcctccgcct cggcttcggc gccttcgccc
      661 ttctcgatca atgggaggga gatgtccgag taggtgtctt cttcgtaggc gccgacgaac
      721 tcgcggaggt ccacctcgtc ggcgacggtg atcttcggct tcgccccgcg cttcgccgtc
      781 gccttcttcg tgtctttctc gtcttctttc tttcgcttcg ccggtccgcg ctcggtcgca
      841 gggggcgggg cggctgcccg gccggggccc atcgggcgtc cgagagatcc ggggcgggca
      901 ccgggtccgc cgggacgaag cggcataccc gcgggacgca ttccgggacc gcccggtgcc
      961 ggccgataga ccgaaggtcc cgggcggccg ggggaaccgg aggcggggcc catcgggcgg
     1021 ccgggggaac cggaagccgg gcccatcggg cggcccggac cggtcggtcg gggagcgccg
     1081 gcgcgggaca tgcctgccgg gccgccgggg gacaccgggc gcgcgacggc cggccgcggc
     1141 ggcgggagaa cgatcttgcg cggcagaggc ggcttcgccg gaggcggcgg ctccgcctcg
     1201 ggctcggagg gaacgacggc ggtacgcgcc ggctcggcgg gtttcgattt tgcctcgacg
     1261 ggcggagctg gcggcgccgc cacgggacgc accggcggag gaggaggcgg aggagacaat
     1321 tccggctccg gttcgggacg ggacggcgcc gcgggggcca ggggcttctc gacgatcttg
     1381 atgcgcgaga gcgcgctctt ctgcggacga aacgccttcg actccgcatc gcggacgatg
     1441 agctgcttgg gagcgtgcgt ctttccctga aggatggcga gcaccgtcgc ttcgtcgagg
     1501 gccgcttgcg gcgacttgac gtcgactccg atcgattgaa ggaggaacag gacctccttc
     1561 gtccccacgc ccatctgcgc cgccagctct tctacccgga tcttcgccat cagccttcga
     1621 ttactcctga acgatcgtct cttctgccgg ctggggctca ccgcccgctt cttcgccgga
     1681 tgcttcaccc gtgtcttcgg ccgccgcctc gctctcctcc tcggccccgg cttcttcgtc
     1741 ggccggccgc gggttcctga cttcgtcctc ggcggcgcgc gcggcttcga ggatcttctc
     1801 cgcggtcgct gcgccgatgc cgggaatctc cgaaagctcc gcgacgccct tttcgaggag
     1861 ctcggacagc gatccgattc ccgccgaggc cagccgctcg gccgccttcg gggtgatgcc
     1921 cgggacgtcg ccgacggcca cctgcttcag acgcgacgcc atcagcatcc gcgtcagcgc
     1981 ttccgcgacc tctcccttga cctccgtctc gcccttgatc tcgatcttca tcccgatgag
     2041 cgcggaggcg agccgcacgt tctgtccctt tttcccgatc gcgagcgaca gctggtcgtc
     2101 ctcgacgacg cagtcgagac tcatggtcgt gtcctcgtgg tggatcgggg agacgcgcgt
     2161 gatcttcgcg ggagcgaggg cattctgcgc gaaggtgatg aggtcgtcgg aatagaggat
     2221 gacgtcgatt ttttcgccgt ggagctcccg catgatcgac tgcacgcggc ttccgcgcat
     2281 tccgacgcag gcgccgaccg gatcgacgtc gcg
//
LOCUS       DASWAV010000424         1880 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U2665
            k141_103614, whole genome shotgun sequence.
ACCESSION   DASWAV010000424 DASWAV010000000
VERSION     DASWAV010000424.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960316
            Sequence Read Archive: SRR10260007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 1880)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1880)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:47:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,929
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1880
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_103614"
                     /isolate="SMAG_U2665"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.36444 N 132.31889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..623)
                     /locus_tag="VF958_08795"
     CDS             complement(<1..623)
                     /locus_tag="VF958_08795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9149243.1"
                     /translation="MRRLTTLAFALLVSASSFAATRAVDDPRPLEAKPGVAYRHYILS
                     ARRLLNEDEQRDLAKRGVFIQSSVAGGRYLVAVSPDSTVDDSDPRVARLEPLTLESRV
                     HRTALREVATGKPYLHLNVQFHDDVAFAQAKSAIEAAGGALEDVLQQNFSAAHRLRVV
                     IPSSSVKTLGSDERVLFVFGPLRLEIAPENQTSALVSNVTPLFSAPY"
     gene            complement(672..757)
                     /locus_tag="VF958_08800"
     tRNA            complement(672..757)
                     /locus_tag="VF958_08800"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(722..724),aa:Leu,seq:caa)
     gene            complement(860..>1880)
                     /locus_tag="VF958_08805"
     CDS             complement(860..>1880)
                     /locus_tag="VF958_08805"
                     /inference="COORDINATES: protein motif:HMM:NF012885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HEX9149244.1"
                     /translation="AREIVEAGLPIGANCAARTVRADVEPIARVSQEVGIAIEACTFI
                     GSSPIRQFTEEWNIDWIIEQSKKAVRYATENGIPVMYVTEDTTRAKPEDVEKLYTAAI
                     EAGAKRVCIADTVGHATPWGARSIVRFVRGIVERVNPDVKVDWHGHQDRGLGVINSIA
                     ALEAGADRVHGSAAGIGERVGNTPMDLLMVNLKLMGWIDNDLTALREYVSFVTTATRV
                     PLPDQYPVFGRDAFRTGTGVHAAAIIKAKKKGSEWLADRVYSGVPAGMFGLQQIIEVG
                     PMCGLSNVIFWLDANGYPQEESLAKQLFQLAKSTNRVLTDEELHACVERWREEGDSGL
                     GVRSS"
BASE COUNT          332 a          596 c          588 g          364 t
ORIGIN      
        1 ttgtacggtg cgctgaagag cggcgtcacg ttcgagacga gagcggaggt ctgattctcc
       61 ggcgctatct ccagacgaag gggtccgaag acaaagagga cgcgctcatc agaaccaagc
      121 gtcttcacgg aggacgacgg aatgacgacg cgcaggcggt gcgctgcgct gaagttctgc
      181 tgcagaacgt cctcgagcgc cccccctgct gcttcgatgg cgctcttagc ctgggcgaat
      241 gcgacgtcgt cgtggaactg cacgttgaga tggaggtacg gcttgcccgt agcgacctcg
      301 cgcagtgcgg tgcggtgcac acgactttcc agggtgagcg gctcgagccg cgccacgcgc
      361 gggtcggagt cgtcgacggt cgaatcgggg gaaacggcca ccagatatcg tccgccggca
      421 actgagcttt ggatgaagac tccccgcttt gccaggtccc tctgctcgtc ttcattcaac
      481 agccgccgcg cagagaggat gtagtgccga taagccacac ccggcttcgc ttcgagcggg
      541 cgcggatcgt cgacggcacg ggtcgccgca aagctgctgg cggaaacgag gagggcgaaa
      601 gcaagcgtcg tcagtcgtcg catagttcgt ggttgatacg ttcggatcgt gaatctcgtt
      661 cactccgtgg ctggtgcccg aggccggact cgaaccggca cgcccttgcg agcggtggat
      721 tttgagtcca ctgcgtctgc catttcgcca ctcgggcctg atgcaattgc aatgatcaaa
      781 gagattcggg attcgagatt cgggattcgg gattcggatg tcggactatc tacgaatctc
      841 gaatctccaa tctcgaaacc tacgaacttc gaactcccaa tccggaatcc ccctcttccc
      901 gccaccgctc gacacaggcg tgcaactcct catccgtcag cacccgattc gtcgacttcg
      961 ccagctgaaa gagctgcttg gcgagcgact cttcctgagg atagccgttc gcgtcaagcc
     1021 agaagatcac gttcgacaga ccgcacatcg gtccaacttc aatgatctgc tgcaggccga
     1081 acattcctgc cggtacgccc gagtaaaccc ggtcggccag ccattcactc cctttcttct
     1141 tcgctttgat gatggcggcg gcgtggacgc ccgtgccggt gcggaaggcg tcacgtccga
     1201 acacagggta ctgatcgggc agcggaacgc gtgtcgccgt ggtcacgaaa ctgacatatt
     1261 cgcggagggc cgtcaggtcg ttgtcgatcc accccatcag cttgaggttg accatgagca
     1321 ggtccatcgg cgtgtttccg acgcgctcgc cgatacccgc cgccgagccg tgcacgcggt
     1381 cggctcccgc ttccagcgcg gcgatggagt tgatcacgcc caggccgcgg tcctggtgtc
     1441 catgccagtc caccttcaca tccgggttca cgcgttcgac gatgccccgg acgaagcgga
     1501 cgatgctgcg cgctccccac ggcgtggcgt gtccgacggt atcggcgatg cagacgcgtt
     1561 tcgcccccgc ctcgatggcg gccgtgtaga gcttctcgac atcttccggt ttcgcccgcg
     1621 tcgtgtcctc ggtgacgtac atgaccggga tcccgttctc ggtggcgtag cgaacggcct
     1681 tcttcgactg ttcgatgatc cagtcgatgt tccactcctc ggtgaactgg cggatgggcg
     1741 aggaaccgat gaacgtgcag gcctcgatgg cgatgcccac ctcctgcgac acgcgggcga
     1801 tgggctcgac gtcggcccgg accgtgcgag cggcgcagtt cgcgccgatg ggcagcccgg
     1861 cttccacgat ctcgcgggcc
//
LOCUS       DASWAV010000425         5613 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U2665
            k141_342633, whole genome shotgun sequence.
ACCESSION   DASWAV010000425 DASWAV010000000
VERSION     DASWAV010000425.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960316
            Sequence Read Archive: SRR10260007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 5613)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5613)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:47:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,929
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5613
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_342633"
                     /isolate="SMAG_U2665"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.36444 N 132.31889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1137)
                     /locus_tag="VF958_08810"
     CDS             complement(<1..1137)
                     /locus_tag="VF958_08810"
                     /inference="COORDINATES: protein motif:HMM:NF005744.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HEX9149245.1"
                     /translation="MRARTDSLRADGMKKDGAVAGTEVARPRVSARGEGMPASPIRKL
                     MPLAEEAKRQGVRVLHLNIGQPDLETPPAMRDRLARYSDRVVAYTPSGGTPEYLAYLS
                     EYYGRLGLSLTPEELIATTGGSEAILFAFLSCADPGDEILTVEPFYTNYSAFAAMAAV
                     RLVPLTTRVEDGFHLPPREQWERACTSRTRMVLLCNPNNPTGTVYSPDELAGVAAFCQ
                     DRGLFLVCDEVYREFVYDGRRTVSALSLEGAGDSVIVVDSLSKRYSACGIRLGCLATR
                     NRGVYRAALRMAQGRLSPPGLAQFMAVGALDLPPDYMQQVVAEYERRRNVLYEGLRRI
                     PGVFLTQPEGAFYFVARLPVADGDDFAAWMLTDFHRGGATVMVSP"
     gene            complement(1121..2635)
                     /locus_tag="VF958_08815"
     CDS             complement(1121..2635)
                     /locus_tag="VF958_08815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016862934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HEX9149246.1"
                     /translation="MSTEVALQLSGNFIDGEWMPSASGRTLDNRNPADSDDLVGVFPE
                     SGREDVEAAVAAARQAFPGWRDTPAPRRAEVLFAAGEILRRDKETLARLMTREMGKVV
                     AEAGGDVQEAIDMAYLAAGEGRRLFGFTTPSELSNKFAMCVRQPIGVCALVTPWNFPM
                     AIPAWKSMAALIAGNTIVIKPATDTPASVVALARVFEEAGLPPGVFNVVIGSGGEVGD
                     PLVGHPDVKVVSFTGSTEVGRRISSACAPTFKRVHLEMGGKNAILVLEDADVDLAVEG
                     AVWGAFGTTGQRCTASSRLLVHEEVVEEFTEKLVARARSLKVGNGLEPGVQVGPCISE
                     RQRETVARYVEIGKREGAKLLAGGARLTEGGFARGFFFEPTVFGEATPDMRISREEIF
                     GPVTTIIPIGSFEEGVRVANSVEYGLSGSVYTRDVDRAFRAMREFETGIFYVNASTIG
                     AEVHLPFGGIKNTGNGHREAGIAGIEVFTEWKSIYVDFSGRLQRAQIDTHESAD"
     gene            complement(2632..3189)
                     /locus_tag="VF958_08820"
     CDS             complement(2632..3189)
                     /locus_tag="VF958_08820"
                     /inference="COORDINATES: protein motif:HMM:NF016408.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymer-forming cytoskeletal protein"
                     /protein_id="HEX9149247.1"
                     /translation="MAFFGKPDTPAPPHHPSPSSPAPAERIPAPPTPAPGPAVASAAA
                     TVIGARIKLVGDLSGDEDILINGRVEGKIRVDRKVVVSAGAEIEGDVQAKSVVIGGRV
                     NGQVLASDRAELLPSGRVQGNVHAPRIVMAEGAQIQGRVVMPTDAATRPLSPPPKVDE
                     TPKKPVAPPKPEDAARPPALPGSGG"
     gene            3415..3939
                     /locus_tag="VF958_08825"
     CDS             3415..3939
                     /locus_tag="VF958_08825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9149248.1"
                     /translation="MSTTLSWNSGAGADDAPGFARLTGFGPGFFFARVCVPVNAGATY
                     SWGGFLRLSAPLDYTAQFVLHFCADSSCGESSSISTVPGPTLDGSSGSSGWYLRAGPD
                     VVAPALAQSVAFEASGASPNSLRRVDFDNVYFGPQGTVPPTAAFSIPTVSEAAFSVFA
                     LLLALAGAWRIAKI"
     gene            complement(4397..4472)
                     /locus_tag="VF958_08830"
     tRNA            complement(4397..4472)
                     /locus_tag="VF958_08830"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4437..4439),aa:Gly,seq:ccc)
     gene            complement(4533..4799)
                     /locus_tag="VF958_08835"
     CDS             complement(4533..4799)
                     /locus_tag="VF958_08835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014447689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin family protein"
                     /protein_id="HEX9149249.1"
                     /translation="MIRLTLLSRPGCGLCEEMRREVDALLAGRPHEWEVVDVDSDPEL
                     ARRYGDSIPILFVNGHLFAKIRLPRLSSKLRLLRAASGAASASP"
     gene            complement(4796..5365)
                     /locus_tag="VF958_08840"
     CDS             complement(4796..5365)
                     /locus_tag="VF958_08840"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:NF013250.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HEX9149250.1"
                     /translation="MKGLWTLPNFITLVRLAALPFFLLSIAEGRFGFALGLFVAAGIS
                     DGVDGYLARRFQMNSALGAYLDPIADKLLLMSSYLFLAIPSYPASVKVPIWLAMLVLS
                     RDFLMLLVALLLILTSNKRRFPPTWLGKVTTVTLIVTLVFVLCANVWTWPHEIVLIAF
                     GAAATGTVLSGFDYVVGVVRTPPGPPEAA"
     gene            complement(5362..>5613)
                     /locus_tag="VF958_08845"
     CDS             complement(5362..>5613)
                     /locus_tag="VF958_08845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9149251.1"
                     /translation="ASRETVTRLLRQFAERELIRMRGSAVWIRDEPSRATPTGIRRML
                     EARPETPRRAAAPSPGKEPSRKSAPSSRRGDEGEGGRRR"
BASE COUNT          939 a         1865 c         1907 g          902 t
ORIGIN      
        1 gggcgacacc atcacggtgg cgccgccgcg atggaagtcc gtcagcatcc acgcggcgaa
       61 gtcgtcgccg tcggcgacgg gaaggcgagc gacgaagtag aacgcgcctt ccggctgcgt
      121 caggaagacg cccggaatcc ggcggagccc ctcgtagagc acgttgcggc gccgctcgta
      181 ttcggcgacg acctgctgca tgtagtcagg cggaagatcc agcgctccga cagccatgaa
      241 ctgggcgagg cccggcggag agaggcgtcc ctgcgccatc cgcagagcgg ctcggtagac
      301 tccgcggttt cgcgtcgcga ggcatccgag ccggatgccg caggcgctgt agcgcttcga
      361 caggctgtcg acgacgatga cggaatcccc ggcgccctcg agcgagagcg cgctgacggt
      421 ccggcggccg tcgtagacga actcgcggta gacctcgtcg cacaccagga agaggccgcg
      481 gtcctggcag aaggcggcga cgccagcgag ctcgtcggga gagtagaccg tgccggtcgg
      541 gttgttcggg ttgcacaaca ggaccatccg ggtccgcgat gtgcacgcgc gctcccactg
      601 ctcgcgaggt ggcaggtgaa atccgtcttc gacccgagtc gtcagcggga cgagccggac
      661 ggcggccatc gcggcgaacg cggagtagtt cgtgtagaac ggctccacgg tcaggatttc
      721 gtcccccgga tccgcgcagg agaggaaggc gaacaggatt gcctcgctgc ctcccgtggt
      781 cgcgatcaac tcctccgggg tcagagagag cccgaggcgt ccgtagtact cggagagata
      841 tgcgaggtat tccggtgtcc cgccggaggg cgtgtacgcg acgacccgat ccgagtagcg
      901 cgccagacgg tcgcgcatcg ccggcggggt ctccaggtcc ggctggccga tgttcaggtg
      961 caggacgcgg acgccctgcc gtttcgcctc ctcggcgagc ggcatgagct tgcggatcgg
     1021 cgaggccggc atgccttcgc ctcgcgccga cacgcggggt cgcgcgacct ccgtccccgc
     1081 cacagcgccg tccttcttca tcccgtccgc gcgaagcgaa tcagtccgcg ctctcatggg
     1141 tatcgatctg cgcacgctgg aggcgacccg aaaagtccac gtagatggac ttccactccg
     1201 tgaacacctc gatccccgcg attccggcct cccggtggcc gttgccggtg ttcttgatcc
     1261 cgccgaacgg caggtggacc tcggcgccga tcgtcgacgc gttcacgtag aagattccgg
     1321 tctcgaactc gcgcatcgcg cggaaagccc ggtcgacatc gcgcgtgtac acggagcccg
     1381 agaggccgta ctcgacggag ttcgcgacgc ggacgccctc ttcgaacgaa ccgatcggaa
     1441 tgatcgtcgt cacggggccg aagatctctt cgcgcgaaat cctcatgtcc ggcgtcgcct
     1501 ctccgaagac cgtcggctcg aaaaagaatc cccgggcgaa cccgccttcc gtcagcctcg
     1561 cgcctccggc caggagcttc gcgccctcgc gttttccgat ctcgacgtag cgcgcgaccg
     1621 tctcgcgctg gcgttcggag atgcagggac cgacctgaac cccgggctcg agcccgttgc
     1681 cgaccttgag cgaccgcgcc cgcgcgacga gcttctccgt gaactcctcg acgacctcct
     1741 cgtgcacgag gagacgcgat gatgccgtgc agcgctggcc cgtggtgccg aaggcgcccc
     1801 agaccgcgcc ttccacggca aggtccacgt ccgcgtcctc gagaaccagg atcgcgttct
     1861 tgccccccat ctcgaggtga acgcgcttga aggtcggcgc gcaggcagag gagatccgac
     1921 ggccgacctc cgtcgagccg gtgaacgaca cgaccttcac gtccggatgc ccgacgagag
     1981 gatcgccgac ctcgccgccc gacccgatca cgacgttgaa gacgccggga ggcaaccccg
     2041 cctcctcgaa cacgcgggcg agcgccacga ccgacgcggg cgtgtcggtc gcgggcttga
     2101 tcacgatcgt gttgccggcg atcagcgcgg ccatcgactt ccacgccggg attgccatcg
     2161 ggaagttcca gggcgtgacg agagcgcaga caccgatcgg ctgccgcacg cacatcgcga
     2221 acttgttcga aagctcggaa ggagtcgtga agccgaacaa ccgccggccc tcgcccgccg
     2281 cgaggtaggc catgtcgatg gcttcctgga cgtcgccgcc ggcttcggcg acgaccttgc
     2341 ccatctcccg cgtcatcagc cgcgcgagcg tctccttgtc gcgccgcagg atctcgccgg
     2401 ccgcgaagag gacctcggcg cggcgcggcg ccggcgtgtc gcgccatccc ggaaacgcct
     2461 gtcgcgcggc cgcgaccgcg gcttcgacgt cttcgcgacc gctctcgggg aagacgccga
     2521 cgaggtcgtc ggagtccgcc gggttgcgat tatcgagggt gcgacccgac gccgacggca
     2581 tccactcgcc gtcgatgaag ttcccggaga gctggagggc gacctcggtg ctcatccgcc
     2641 gctgcccggt aacgccggcg gacgcgccgc gtcttcgggt ttgggcggag ccaccggctt
     2701 cttgggggtt tcgtcgactt tcggaggggg cgagagggga cgtgtcgccg cgtccgtggg
     2761 catcaccacg cgaccctgga tctgggcccc ttccgccatc acgatccggg gcgcgtggac
     2821 gttgccctgg acccttcccg acggcaggag ctccgcgcga tccgacgcca gcacctggcc
     2881 gttgacccgg cctccgatca cgaccgactt cgcctgcacg tccccttcga tttcggcgcc
     2941 ggccgagacg acgaccttcc ggtcgacccg gatctttcct tcgacccggc cgttgatcag
     3001 gatgtcttcg tcaccggaga ggtcgccgac gagcttgatc ctggcgccga tcaccgtcgc
     3061 cgcggccgaa gcgaccgccg gacccggcgc cggggtgggc ggggccggaa ttcgttccgc
     3121 gggagccgga gaagacgggc tcggatggtg ggggggagcc ggggtgtcgg gtttgccgaa
     3181 gaatgccaat ggtaggtcct ccgaaaaaga gtcagtatag acccgctcgc gagccggcga
     3241 cgccgctttt cgtaaagaag cgcggaggca gagatgaggt accgctgtgt ctcagtcgtg
     3301 agcaccctcg cgctgctttt cgcggtgcat tccgccgggg cccagaacct catcccggat
     3361 ccgacatttt cctcgggcat atccgcgtgg gcgatctggg acccgtctgg gacgatgagc
     3421 accacgctga gctggaattc cggcgcgggc gccgacgatg ctcccggctt cgcgaggctg
     3481 accggattcg ggccgggctt cttcttcgcg agggtgtgcg ttccagtcaa cgcaggagcg
     3541 acttactcct ggggcggctt tctccggctg tcggcacctc ttgattacac cgcgcagttc
     3601 gtgctgcact tttgcgcgga ctcatcctgt ggagagtcat ccagtatttc gacggtcccc
     3661 ggtccgactc tcgacggctc cagtggaagc tccggctggt atctgcgagc tgggccggac
     3721 gtagtggcgc ccgcactcgc gcagagcgtt gctttcgaag ccagtggggc ctcgccgaat
     3781 tctttgcgcc gcgtcgactt cgacaacgtc tacttcggcc cgcagggaac ggttccgcca
     3841 acggcggctt tctccatccc caccgtgtcg gaagcggcgt tctcggtctt cgcccttctt
     3901 ctagccctcg ccggtgcatg gcgaatcgcg aagatttgaa tgatcaactg tcgctgtcgt
     3961 ggcgcggcgt cattatgtgc agccaatgaa gaagaacctg cttccgggtt tcagcaacgc
     4021 gcgtcggaaa atcgcggagt tccgtgtgat cgttccgaga accggtgcga accggcggcg
     4081 gaggctcacg gtctctgcgg aaactcgcga cgaggcgcta tcgaagtgga aagcgtttcg
     4141 cgatccgcta ctcgcggggc aggcggaacc tctccccttc tcggttcatg ttgagcgcgt
     4201 gtggccgctg ctgcgagcgc ggctctctca gcgcaaggga cacgcttcgg cgcggactac
     4261 ggagcggtat gtgagggtgt cggaagaatc gttgcgggag atcgcttcgg cgctggattc
     4321 ggtgaattcg gccttttttt cggacccttc gcgggtagtg aatcagtcgg gttgagtcgc
     4381 aagtcgctga aaagattgga gcgggcaacg gggttcgaac ccgcgaccct cagcttggga
     4441 agctgatgct ctaccaactg agctatgccc gcccggtcgc gtgaaatata gcggaacggc
     4501 ggcgagtccg gcaacgcgcc gcgcgcggcg ctttagggag aagcggaagc cgcgcccgac
     4561 gcggcccgca ggagcctcag cttcgaggag agcctcggca gccggatctt ggcgaagaga
     4621 tgtccgttga cgaagaggat ggggatcgag tcgccgtaac gccgcgccag ctccgggtcg
     4681 gagtccacgt ccacgacctc ccactcgtgc ggccgccccg cgaggagagc gtcgacctcg
     4741 cggcgcattt cctcgcagag accgcagccg ggccgcgaca gcagcgtcag ccggatcacg
     4801 cggcctccgg gggtcctggc ggcgtgcgga cgaccccgac gacgtagtcg aaccccgaca
     4861 gcacggtccc ggtcgccgcc gctccgaaag cgatcagcac gatctcgtgc ggccaggtcc
     4921 agacgttcgc acacaacacg aagacgaggg tgacgatgag agtgaccgtc gtgaccttgc
     4981 cgagccacgt cggcggaaaa cgacgcttgt tcgacgtgag gatgaggagg agcgccacca
     5041 gcagcatgag gaagtcgcgg gagaggacga gcatcgcgag ccagatcggg accttcacgc
     5101 tcgccggata ggacggaatg gcgagaaaca ggtacgagct catgagcagg agcttgtcgg
     5161 caatcggatc gagataggcg ccgagtgccg aattcatctg gaaccgccgg gcgagataac
     5221 cgtccacgcc gtccgagatt cccgcggcga cgaagagacc aagggcgaaa ccgaaccgcc
     5281 cctccgcaat cgagagtaga aagaatggaa gcgccgcgag gcggacgaga gtgatgaagt
     5341 tgggaagagt ccagaggccc ttcaccggcg gcggccgccc tccccctcat cgccgcggcg
     5401 cgacgaaggc gccgatttcc gtgagggttc ctttcccggc gacggggcgg cggcgcgacg
     5461 cggcgtctcc ggccgcgctt ccagcatgcg ccggataccg gtgggggtcg cccgactcgg
     5521 ctcgtcgcgg atccagaccg ccgaaccgcg catccggatg agctcccgct ccgcgaactg
     5581 ccggagcagc ctcgtgacgg tctcgcgcga agc
//
LOCUS       DASWAV010000426         4212 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U2665
            k141_223930, whole genome shotgun sequence.
ACCESSION   DASWAV010000426 DASWAV010000000
VERSION     DASWAV010000426.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960316
            Sequence Read Archive: SRR10260007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 4212)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4212)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:47:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,929
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4212
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_223930"
                     /isolate="SMAG_U2665"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.36444 N 132.31889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..597)
                     /locus_tag="VF958_08850"
     CDS             complement(<1..597)
                     /locus_tag="VF958_08850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012811137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEX9149252.1"
                     /translation="MRSFASVGGEPFFVTSGRGARLRDADGVSRLDYVMSYGPLLFGH
                     APPFVVREVARAARGGTSFGAPTEREVRLAERVTRMFPSVEMVRFVNSGTEAAMSAIR
                     LARGATGRARIVKTDGGYHGHADSFLVSAGSGVATLGIAGSPGIPPELALLTTVVPYN
                     DGDALEEAFRRHRGEIAAVVLEPVAANMGVVPPRPGYLE"
     gene            complement(694..1686)
                     /gene="hemB"
                     /locus_tag="VF958_08855"
     CDS             complement(694..1686)
                     /gene="hemB"
                     /locus_tag="VF958_08855"
                     /EC_number="4.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012937383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004655 - porphobilinogen synthase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porphobilinogen synthase"
                     /protein_id="HEX9149253.1"
                     /translation="MTGFPTIRMRRLRRTPALRDLVRETRLSADRFIAPLFVRRGSGV
                     REEISSLPGVFRLSPEQAVEEARELRKVGVPAVILFGIPDRKDDVGSEGWDDTAAVQE
                     TVRQLKDSVPELVVMTDVCLDEYTSHGHCGVIKDGEVDNDATLPLLARIAVSHARAGA
                     DVVAPSDMMDGRVAAIRRTLDAEGFTAVAILAYAAKSASAFYGPFREAADSAPKFGDR
                     RGYQMDPANRREAMREIELDLQEGADAVMVKPALSYLDIIREARNRFDAPIAAYNVSG
                     EYAMVKAAAANGWIDEKRIVGEILTSIVRAGADFVLTYHARDAARWLAEGSLPH"
     gene            complement(1705..2427)
                     /locus_tag="VF958_08860"
     CDS             complement(1705..2427)
                     /locus_tag="VF958_08860"
                     /EC_number="4.2.1.75"
                     /inference="COORDINATES: protein motif:HMM:NF014641.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004852 - uroporphyrinogen-III synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006780 - uroporphyrinogen III biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006782 - protoporphyrinogen IX
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III synthase"
                     /protein_id="HEX9149254.1"
                     /translation="MKVGLLVVRSGERAFPSSLVPDIEVLERRSHAIETLPAKPPGGP
                     FDLAIFTSRSAADRFLAREDLLATLPGRVLAVGSATAERFRSGLSVDVEEGGGSVREM
                     LRRLPRNLAGTRVLVPHGEDSDPELARELADRSAQVVSLLLYRKVALPYDPELDSSIM
                     TLLPVAFFATSPSAARWLLDGAGESARVRLTRTIAIVLGEATRKALSARRVARIEVAR
                     PPTFERVARIAAELGGRPPLAR"
     gene            complement(2429..3334)
                     /gene="hemC"
                     /locus_tag="VF958_08865"
     CDS             complement(2429..3334)
                     /gene="hemC"
                     /locus_tag="VF958_08865"
                     /EC_number="2.5.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013495679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004418 - hydroxymethylbilane synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylbilane synthase"
                     /protein_id="HEX9149255.1"
                     /translation="MKIRIGARGSALSLTQTELVARALREGAEVEVVPIRTTGDRLSR
                     LEAAMTGKDLFTREIDEALLGGRIDVGVHSLKDLPSRLPDRLTLAAVPRREDPSDVLV
                     SRQRKTLASLPPGARVGTSSARRRAQVLAARPDAEVLEARGNIDTRIRRLDEERWDAI
                     VLARAGLARLGRLDEVAEVLPFSTMLPAIGQGALAIVAREDDPAVRDRVSRLDDLVSH
                     REVRAERALLDLLEAGCRAPVAGLARVEGDRLRLAGAVFAPDGSRTLREEAEGPAEDP
                     ERLGREVAERLLARGAASLIALARG"
     gene            complement(3344..>4212)
                     /gene="hemA"
                     /locus_tag="VF958_08870"
     CDS             complement(3344..>4212)
                     /gene="hemA"
                     /locus_tag="VF958_08870"
                     /EC_number="1.2.1.70"
                     /inference="COORDINATES: protein motif:HMM:TIGR01035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008883 - glutamyl-tRNA reductase activity
                     [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glutamyl-tRNA reductase"
                     /protein_id="HEX9149256.1"
                     /translation="AQETGTTRAVLHRLFEGAVHAGKRVRTETEIAVHPLSLASIAVE
                     LADKVFGNLPERTLLLLGSGETAALFARHALEAGVRDLRIANRTREHAVDLAARIGAR
                     VVAWESVAKELPMADIVVGATASPDPVVLRADVEAAMRQRRGRPMFFLDLAMPLDIDP
                     SVKDIYNVFVYDLDGLEEVATENRRRRVREVPRAEEILEEELRRFLGWYGNLSVIPTV
                     TDLKRRLEEIRDGELARLSPEDRERLRPLADSIVAKLLHEPMRRLKGETDSSRKIDRV
                     EAVRHLFNLDRE"
BASE COUNT          682 a         1479 c         1444 g          607 t
ORIGIN      
        1 ctcgagatac cccggccgcg gcggcacgac acccatgttc gcggcgaccg gctcgagaac
       61 gaccgcggcg atctcgccac ggtgccggcg gaaggcctct tcgagtgcgt cgccgtcgtt
      121 gtaaggcacg accgtggtca gaagggcgag ctccgggggg atgccgggac ttcccgcgat
      181 tccgagcgtc gcgacccccg acccggcgga gacgagaaat gagtcggcgt ggccgtgata
      241 cccgccgtcg gtcttgacga tccgtgcgcg gccagtcgca ccgcgcgcga gccggatcgc
      301 gctcatggcc gcctcggtcc cggagttcac aaaccgtacc atctcgacag aggggaacat
      361 ccgcgtgacc cgctcggcga gcctcacctc gcgttcggtc ggagcgccga aggacgtccc
      421 gccgcgcgcc gcccgggcga cctcgcgcac gacgaacggc ggcgcgtgtc cgaagaggag
      481 cgggccgtag gacatgacgt agtcgaggcg tgagactccg tcggcgtccc ggagccgcgc
      541 gccccggccg ctcgtgacga agaaaggttc tccaccgacc gaggcgaacg accgcacggg
      601 cgaactgact ccgcccggca tgagcttgcg cgcccgcttg aacagtgcgg acgagcgctc
      661 gactctggat ttcgaacttc ggactttcga ctcctagtga ggcagcgagc cttcggccaa
      721 ccagcgggcc gcgtctcgcg cgtgatacgt gagcacgaag tccgcgccgg cgcgcacgat
      781 cgacgtcagg atctcaccga cgatccgctt ctcgtcgatc cagccgtttg cggcggccgc
      841 cttgaccatc gcgtactctc ccgagacgtt gtaggcggcg atcggcgcgt cgaaacgatt
      901 gcgcgcctcg cgaatgatgt cgaggtaaga gagcgcgggt ttcaccatca cggcgtccgc
      961 gccttcttga aggtcgagct cgatttcgcg catggcctcg cgccgattcg ccgggtccat
     1021 ctggtatccc cgccggtctc cgaacttcgg cgccgagtcc gccgcctccc ggaacgggcc
     1081 gtagaacgcc gacgccgact ttgcggcata ggcgaggatc gcgacggcgg tgaacccctc
     1141 cgcgtcgagc gtccgtcgga tcgcagcgac gcggccgtcc atcatgtccg aaggcgccac
     1201 gacgtctgcg ccggcgcggg catgcgaaac cgcgatccgg gcgagcaacg gcagagtcgc
     1261 gtcgttgtcc acttcgccat ccttgatgac gccgcagtgt ccgtgtgacg tgtactcgtc
     1321 gaggcagacg tcggtcatca cgacgagctc cgggacggag tccttcaact ggcggaccgt
     1381 ctcctgaacc gccgcggtgt cgtcccaccc ctcgctgccc acgtcgtcct ttcgatccgg
     1441 gattccgaac agaatgaccg ccgggacacc gaccttgcgc agctcgcggg cctcctcgac
     1501 ggcctgctcc ggagacaggc gaaagacccc gggaagcgac gagatctcct cgcgcacccc
     1561 ggagccgcgg cggacgaaga gcggcgcgat gaaccggtcg gcggagagac gagtctcgcg
     1621 cacgaggtct cgcagcgcgg gcgtgcgacg gagccggcgc attcggattg tcgggaagcc
     1681 ggtcacagag cggaatctag caggttaccg ggcgagcggg gggcgcccgc cgagctcggc
     1741 ggcaatccgc gcaactctct caaaagtcgg cggccgcgcg acctcgattc gggcgaccct
     1801 ccgggcggag agcgccttcc gggtcgcctc gccgagaacg atcgcgatcg tgcgcgtcag
     1861 gcggaccctg gcagactccc ccgccccatc caggagccat cgagccgccg agggggacgt
     1921 cgcgaagaac gcgacgggca agagcgtcat gatcgaagaa tcgagctccg gatcgtatgg
     1981 gagcgcgacc ttccgataga ggagcaggga gacgacctgc gcgcttcggt cggccagttc
     2041 ccgggcgagc tccgggtcgg agtcctcccc gtgcggcacg agcacacgcg tgcccgcgag
     2101 attccgcggg agccgacgaa gcatctcgcg caccgagcca ccgccttctt cgacgtccac
     2161 ggagagtccg gaacgaaacc gttcggccgt cgcactcccc acggcgagca cccgacccgg
     2221 aagagtcgca agcagatcct ctcgcgcgag gaaccgatca gctgcggagc ggctcgtgaa
     2281 gatcgcgagg tcgaagggac ccccaggggg tttcgcaggc agcgtctcga tcgcgtggct
     2341 tcgcctctcg agcacttcga tgtcgggcac gagggacgac ggaaaggccc tctctcccga
     2401 ccgcacgacg agcaatccga ctttcacgtc atccgcgcgc gagcgcgatc agggatgcag
     2461 cgccgcgcgc gagcaggcgc tcggcgacct cgcggccgag cctctcgggg tcttcggcgg
     2521 ggccttccgc ttcctcgcgc agcgtgcggg acccgtccgg agcgaaaacc gcgcccgcga
     2581 gccgcagccg gtcgccttcg acccgtgcga gaccggcgac gggagcgcga catcccgctt
     2641 cgaggagatc gagcagagca cgctcggcgc gcacctcccg atgagagacg agatcatcga
     2701 ggcgcgagac gcgatcacga actgcgggat cgtcttcccg cgccacgatc gccagagcgc
     2761 cctgtccgat cgccggaagc atcgtggaga acggcagtac ctccgcgact tcgtccagcc
     2821 ggccgagccg cgcgagcccg gcgcgggcca gcacgatcgc gtcccatcgc tcctcgtcga
     2881 gccgccgaat gcgggtgtcg atgttcccgc gcgcctcgag aacctcggcg tcgggccgtg
     2941 cggcgagtac ctgcgctcgc cgccgcgcgc tcgacgtgcc tacgcgggcc cccggcggca
     3001 gcgacgcgag ggttttccgc tgtcgcgaaa cgagcacgtc gctcgggtcc tcacgccgag
     3061 ggaccgcagc aagagtcagg cggtcgggca gccgggaggg gaggtctttc aggctgtgca
     3121 ctcccacgtc gatccgaccc ccgaggaggg cctcgtcgat ctcgcgcgtg aacaggtcct
     3181 tcccggtcat cgcggcctcg agacgcgaga gccgatcgcc ggtcgtgcgg atcggaacca
     3241 cttcgacctc ggctccctcg cggagagcgc gggcgacgag ctccgtctgc gtgagggaga
     3301 gggcgctgcc gcgggcgccg attcggatct tcaccggcgg ccgctactcg cgatccagat
     3361 tgaacaggtg tcgcactgcc tcgacccggt cgatcttgcg cgaggagtcg gtctcccctt
     3421 tcaagcgtcg catcggctcg tgcaggagct tcgcgacgat ggagtcggcg agggggcgca
     3481 ggcgttcgcg atcttcgggc gaaagacggg cgagttcccc gtcccggatc tcctcgagcc
     3541 gccgcttcag gtcggtcacc gtcggaatga ccgaaaggtt tccataccag cccagaaatc
     3601 gccggagctc ctcctcgagg atctcctccg cccgcgggac ctcgcgcacg cggcggcggc
     3661 ggttctcggt cgcgacctcc tcgagcccgt cgaggtcgta gacgaagacg ttgtagatgt
     3721 ccttcaccga gggatcgatg tcgagcggca tcgcgagatc gagaaagaac atcggccggc
     3781 cgcgacgctg gcgcatcgct gcttcgacgt ccgcgcgaag cacgaccggg tcggggctgg
     3841 cggtcgcccc gacgacgatg tccgccatgg ggagctcctt cgccacactc tcccaggcga
     3901 cgacgcgggc cccgatccgg gccgcgaggt ccactgcgtg ctcgcgcgtg cggttggcga
     3961 tgcgcagatc gcggactccc gcctcgagcg cgtggcgggc aaagagcgcg gcagtctcac
     4021 cggatcccag gagcagcaag gtgcgctcgg gaaggtttcc gaagaccttg tccgccagct
     4081 cgaccgcgat cgacgccagc gacagagggt ggaccgcgat ctccgtctcc gttcgcaccc
     4141 gcttgccggc atgcacggcg ccttcgaaga gccggtgcag aaccgcccgg gtcgtgccgg
     4201 tctcctgcgc ga
//
LOCUS       DASWAV010000427         2202 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U2665
            k141_464064, whole genome shotgun sequence.
ACCESSION   DASWAV010000427 DASWAV010000000
VERSION     DASWAV010000427.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960316
            Sequence Read Archive: SRR10260007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2202)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2202)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:47:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,929
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2202
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_464064"
                     /isolate="SMAG_U2665"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.36444 N 132.31889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..816
                     /locus_tag="VF958_08875"
     CDS             <1..816
                     /locus_tag="VF958_08875"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: protein motif:HMM:NF014164.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HEX9149257.1"
                     /translation="GDRPMFELPQPVREAFERSEVSIYAGQPAPGELAYRREMTAIVN
                     RRKMRHAHMVSISPRIMAEGMRADFEEVNAISTRLRDRAVKAERIRARSRAGSDLEAT
                     FDPSVKWLKTSGLITPEKWANLPGGEVFTAPARVDGVYVVDGVLGDYLCARYGDLEET
                     PLTVWIEDSRVVRAECARTDVRDDFLEYIDTAENSNRVGEFAMGTNIAVDSIIGNILQ
                     DEKLPGLHLAFGHPYTEHTGASWSCPTHLDIVGRHFDVWFDEDQVMADGKFLI"
     gene            966..2009
                     /locus_tag="VF958_08880"
     CDS             966..2009
                     /locus_tag="VF958_08880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9149258.1"
                     /translation="MVRAATEIWGELSTPENLRRSLEAVPPSLDVPGCTDSPTFAQAD
                     FAVARDEPGGPLVPKLIELQAFASLYAFQIFQSKELMRMTPGGESLDFLLSGLDGTGY
                     ALAVGDAILGGFPAENVVLMDIDPPSQKTFVDFAFTEKLWNVRPVSVMDIERRGRELW
                     YRRDGRATRILRIYNRLIFDELAAKGISIPIDFREPLDVSWAGHPNWYFRWSKHSLPG
                     LAHPSVPEARLLSDFERWPDDLENWVYKPLFSFAGSGVKVDVTRADLDRVPPADRHHG
                     LLMRKVDYAPVIETTDGNASKVEVRVMFVWRDGRPFPVTTLARLSQGPMMGVAFNKDR
                     TWVGSSGCLWPVG"
BASE COUNT          370 a          730 c          725 g          377 t
ORIGIN      
        1 ggcgatcggc cgatgttcga gctgccgcag ccggtgcgcg aagcgttcga gcgctcggag
       61 gtttcgatct acgccgggca accggccccg ggcgagctcg cgtatcgccg ggagatgacc
      121 gcgatcgtga accgccggaa gatgcggcac gcgcacatgg tttcgatctc cccgcggatc
      181 atggccgagg ggatgcgtgc ggacttcgag gaggtcaacg cgatctcgac ccgactgcgc
      241 gaccgcgccg tcaaagccga gcggatccgc gcccgttccc gcgcggggag cgacctcgag
      301 gcgaccttcg acccctccgt gaaatggctg aagacgagcg ggctgatcac gccggagaag
      361 tgggcgaacc tgccgggcgg cgaggttttc accgcgccgg cgcgcgtgga cggcgtctac
      421 gtcgtcgacg gcgttctcgg cgattacctc tgcgctcgct acggcgacct cgaagagacg
      481 ccgctcacgg tgtggatcga agacagccgc gtggtccgcg ccgagtgcgc gcggaccgac
      541 gtccgggacg acttcctcga gtacatcgac accgcggaga actccaaccg cgtcggcgag
      601 ttcgccatgg gcacgaacat cgccgttgac tcgatcatcg gcaatatcct ccaggacgag
      661 aagctgccgg gacttcacct cgcgttcggg cacccctata ccgagcacac cggcgcgtcg
      721 tggagttgcc cgactcacct cgacatcgtc ggccgccact tcgacgtgtg gttcgacgag
      781 gaccaggtga tggccgacgg gaagttcctg atctgatccc ggaaattcgc cgcgcttaca
      841 acgccgcgtt cgatcagcaa aagtacgggg actaccgccg tcggctcgaa gagctggcgg
      901 gctgcgagat ccactttcgc ctctgcgaga cgccggtctt tcttccgccg gcactgcgcg
      961 acgaaatggt ccgcgccgcg accgagatct ggggagagct ctccacgccg gagaacctgc
     1021 gccgctcgct cgaagcggtc cctccgtcgc tcgacgtccc gggctgcacg gactcaccga
     1081 cgttcgcgca ggcggacttc gccgtcgcgc gggacgagcc gggcgggccc ctcgtcccga
     1141 aactgatcga gctccaggcg ttcgcgtccc tttacgcgtt ccagatattt cagtcgaagg
     1201 agctcatgcg catgacgccc ggaggagaga gcctcgactt cttgctctcc ggcctcgacg
     1261 ggacggggta cgcgctcgcg gtgggcgacg cgatcctcgg cgggtttccg gcggagaacg
     1321 tcgtgctgat ggacatcgac ccgccgtccc agaagacgtt cgtcgacttc gctttcaccg
     1381 agaagctgtg gaacgtgcgg ccggtttcgg tcatggacat cgagcggcgc gggcgcgagc
     1441 tctggtatcg ccgggacggc cgcgcgacgc gaattctcag gatctacaac cgcctcattt
     1501 tcgacgagct cgccgcgaaa gggatctcga tcccgatcga cttccgggag ccgctcgacg
     1561 tgtcgtgggc ggggcatccc aactggtact tccgatggag caagcattcg ctccccggcc
     1621 ttgcgcatcc gtccgtcccg gaggcgcgcc ttctctccga cttcgagcgc tggccggacg
     1681 acctcgagaa ctgggtctac aagcctctct tctctttcgc gggttcgggt gtgaaagtcg
     1741 acgtcacgcg cgcggatctc gaccgtgtgc cgccggcgga tcggcaccac gggctgctca
     1801 tgcgcaaggt cgactacgcg cccgtcatcg agaccaccga cggaaacgcc tcgaaggtgg
     1861 aggtgcgggt catgttcgtc tggcgcgacg gaaggccgtt cccggtgacg acgctcgcac
     1921 gcctctcgca gggtccgatg atgggcgtcg ccttcaacaa ggaccggacc tgggtcggct
     1981 cgagcggctg cctctggccc gtcggctgag cgacgcattc atccctcatt catcgccgca
     2041 ccctcacgct ggggtcgaac gaaaggagga tttttcatga ggcgaatcgg aactctcctc
     2101 ttcatcggcc tgtcggtgct cttcttctcc acgctggcgc tcgcgcagga gacgaagacg
     2161 acgaccacga cgaagaagac cgagatgaag atgaagatgg gg
//
LOCUS       DASWAV010000428         5383 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U2665
            k141_424845, whole genome shotgun sequence.
ACCESSION   DASWAV010000428 DASWAV010000000
VERSION     DASWAV010000428.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960316
            Sequence Read Archive: SRR10260007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 5383)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5383)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:47:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,929
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5383
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_424845"
                     /isolate="SMAG_U2665"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.36444 N 132.31889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            183..920
                     /locus_tag="VF958_08885"
     CDS             183..920
                     /locus_tag="VF958_08885"
                     /inference="COORDINATES: protein motif:HMM:NF015734.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PASTA domain-containing protein"
                     /protein_id="HEX9149259.1"
                     /translation="MPRVLFRLIYGAVLAAVFLFAGWIAFQRSIVGRSVSVPDLSGKT
                     VPEAIHIAHDVGLQVEEEAPRARYDDRVPVHRVLLQQPEAGSLAKPGQVVRLVLSLGP
                     RELKVPDLAGLAPRAAALKLARESLQLGTVSWYRDSDVPTGIVAQNPDPDTPATKGST
                     VEVLTNRGLPELRFVMPDFVGQEADRVRARLETFGFRVGSARYEAYEGVAPNTVLKQF
                     PPAGYPLSRRDVVSLTVSQSASAIPAS"
     gene            917..1588
                     /gene="rpe"
                     /locus_tag="VF958_08890"
     CDS             917..1588
                     /gene="rpe"
                     /locus_tag="VF958_08890"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018211016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HEX9149260.1"
                     /translation="MTRVRIAPSLLAADFARLADALATAERGSADLVHIDVMDGHFVP
                     NLTIGPPVVKAVRAATRLALDVHLMIEHPERTVAEYLDAGADWVSVHVEATSHLQRCL
                     ATIRKAGVRAGAAINPGTPPDALSAAWDDLDYVVVMSVNPGWGGQPFLPGSVEKVRRL
                     RLIASESGAHPTIEVDGGVDEGNAPSLAGAGAEILVAGTAIYGAGDPAAAIGRLRAAA
                     EGVRA"
     gene            1585..2418
                     /gene="bamD"
                     /locus_tag="VF958_08895"
     CDS             1585..2418
                     /gene="bamD"
                     /locus_tag="VF958_08895"
                     /inference="COORDINATES: protein motif:HMM:TIGR03302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0065003 - protein-containing complex
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamD"
                     /protein_id="HEX9149261.1"
                     /translation="MRLTKKMHPATRRAMALVAVLLVSFVACSGTKKPDKITQELLAK
                     SKEELFEKGKGLIEKKKYEQGRKYLTFVFETYPNDPLGRDALLMVADSYFKQGGTGYT
                     EARFRYRDYLNRYPAATRRDYARYQFALTYDKEIERPDRDQTATREAIDQYRALIREY
                     PTSSFATAGKERVRVLTDKLAEHEFAVGYFYMRKGAPAAALVRFTELEQRYPEYGARD
                     KLFFYSARALERLGRREEANRYYSRILEEFPESEWARRARGERGGKAAPAAAVAATPK
                     S"
     gene            2566..3510
                     /locus_tag="VF958_08900"
     CDS             2566..3510
                     /locus_tag="VF958_08900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9149262.1"
                     /translation="MRLPARTVTFVLAGVLTAGAGLAQVRARPAQTVPREPQKEIPGG
                     AIVVAPTTQTVVTEGAASVETAPQPLGYESDLHCFGYLGAPRETFVAGVVGAEGVAEQ
                     IDFFTGDLLYVDAGYNRGLKVGDEFWLVTPEQIVVHPTTGKDLGRFYQYRGRATVETL
                     EGRTASVRVSSACSDIPIGSYLKPFEPIPIPLARKTPPAVAGDPPSGKARGHIVYTRD
                     GVVAVGADHTIIVDLGVADGIEPGDFLTIYRYAVGREYGIRPVGAYWVNLPPPPGAEI
                     PRTYLGEISILSVGDRWAIGRVSDSYRLIEVGDEVEVK"
     gene            3700..4002
                     /locus_tag="VF958_08905"
     CDS             3700..4002
                     /locus_tag="VF958_08905"
                     /inference="COORDINATES: protein motif:HMM:NF014950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX9149263.1"
                     /translation="MRASSNALPISEADVNLDSQLQQVIDELLSHGISLDLAKKEFEK
                     KYIVGAVKRSSGNLGRAASSLGIHRNTLRNKVGILNIRFPRDQYRVSVSPDIHKKK"
     gene            complement(4097..4270)
                     /gene="tatA"
                     /locus_tag="VF958_08910"
     CDS             complement(4097..4270)
                     /gene="tatA"
                     /locus_tag="VF958_08910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013505872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase TatA/TatE family
                     subunit"
                     /protein_id="HEX9149264.1"
                     /translation="MFGRLGVPELLIILLIVLVIFGASRLPQLGKGLGQGLKNFKDSV
                     KPEEPGGKNPPPS"
     gene            complement(4295..5263)
                     /locus_tag="VF958_08915"
     CDS             complement(4295..5263)
                     /locus_tag="VF958_08915"
                     /inference="COORDINATES: protein motif:HMM:NF013775.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HEX9149265.1"
                     /translation="MIPAEFAGLQELARRTHYEPCAVEVLPGDVGRRRYLRLTVGGNK
                     TVLGVFYPPEEEDARRRWLAARSVLSGAGVRVPNVYAEDRGGSQILEDFGPEDLATRL
                     AGQPSERSDWLGRALDVAERIAAAPDPRINSPFDAAFFRRELDLAREAVFDLWLGAPL
                     SSDDRAAHDEWAASLTREIEEHPRVLCHRDFHGNNLFPVGDLVAAIDFQDLRSGPDAY
                     DVASLLWERTTLDWMVDDHGEKIVREFAERRGLRAEEFARRLERVLLQRAWKVCGTFA
                     RAVSQGRGETYRRYLPGELGLVRRLLTAPADTRFRTVFEARLGHLC"
BASE COUNT          909 a         1792 c         1762 g          920 t
ORIGIN      
        1 ttgctcggca tctggcttcg ccaccggcgc ccggctcggc aagccggcct cgaccgggcg
       61 cctgatgcct tgcggcgctt gcgctcgcgc ggcgcaaagc tcctccgctc ggttacttcg
      121 cggagactcc tcgcgccgcg accgaggccg ggcgccccgg atttttccgc tatgctctgc
      181 cggtgccgcg ggtgcttttt cggctgattt acggcgccgt cctggcggcc gttttcctgt
      241 tcgcaggctg gatcgcgttc cagcgttcga tcgtgggccg ttcggtgtcg gttcccgatc
      301 tgtccggcaa gaccgttccg gaggcgatcc acatcgcgca cgacgtcggc ctccaggtcg
      361 aggaggaggc gccgcgggct cgctacgacg acagggttcc agtccatcgc gttctcctgc
      421 agcagcccga ggcgggttcc ctcgcgaagc ccgggcaggt tgtccggctc gtcctctcgc
      481 tcgggccccg ggagctcaag gtgccggacc tcgcgggcct ggcgccccgt gccgccgcgc
      541 tcaagctcgc ccgcgaatcg ctccagctcg gaaccgtctc ctggtaccgc gactccgacg
      601 ttccgaccgg catcgtcgcg cagaacccgg atcccgacac cccggcaacg aaggggagca
      661 ccgtcgaggt gttgacgaac cgcggcctgc ccgagctccg cttcgtgatg cccgacttcg
      721 tcggccagga ggccgatcgt gtgcgcgccc ggctcgagac cttcgggttt cgggttggaa
      781 gcgcgcgcta cgaagcgtac gagggcgtgg cgccgaacac ggtcctgaaa cagtttccgc
      841 cggccggata tccgctctcg cggcgcgacg tcgtctctct gaccgtgtca cagtccgcct
      901 cggcgattcc cgcgtcgtga cgagagtccg catcgccccg tcgctgctcg cggcggactt
      961 cgcgcggctg gccgatgcgc tcgcaaccgc ggagcgcggc agcgccgacc tcgtccacat
     1021 cgacgtcatg gacggccact tcgttccgaa cttgacgatc ggcccgccgg tcgtcaaggc
     1081 ggtacgcgcc gcaacgcggc tggcgctcga cgttcatctc atgatcgagc atccggagag
     1141 aaccgtggcc gagtacctcg acgcgggcgc ggactgggtg tccgtgcacg tcgaggcgac
     1201 gagccacctg cagcgctgtc tcgcgacgat ccggaaggcg ggcgtccggg ccggcgccgc
     1261 catcaatccc ggaacgcccc ccgacgcact ctcggccgcc tgggacgacc tcgactacgt
     1321 cgtcgtgatg tcggtgaacc cgggctgggg agggcagccg ttcctgcccg gttcggtcga
     1381 aaaggtgcgg cggctgcggt tgatcgcgtc ggagtctggc gcgcatccca cgatcgaggt
     1441 cgacggcggc gtggacgaag gaaacgcccc gagcctcgcc ggcgccgggg cggaaattct
     1501 cgtcgccgga accgcgattt acggggcagg cgaccccgcg gcggcaatcg ggcggctgcg
     1561 ggccgccgcg gaaggggtcc gggcgtgagg ttgacgaaga aaatgcatcc ggcgacccgg
     1621 cgggcgatgg ctctcgtcgc ggtcctgctc gtttcgttcg tcgcctgctc ggggacgaag
     1681 aagcccgaca agatcacgca ggaactcctc gcgaagtcca aggaggaact cttcgaaaag
     1741 ggcaaggggc tgatcgagaa gaagaaatac gagcagggcc gaaagtacct gacattcgtc
     1801 ttcgagacgt atccgaatga tcccctgggc cgcgacgccc tcctgatggt cgccgactcc
     1861 tacttcaagc agggcggcac gggttacacc gaggcgcggt tccgctaccg cgactacctg
     1921 aaccgctatc ccgccgcgac ccggcgcgac tacgcccgtt accagttcgc gctgacctac
     1981 gacaaggaga tcgagcggcc cgaccgcgac cagacggcca caagggaagc gatcgaccag
     2041 taccgggctc tcatccggga gtacccgacg tcttcgttcg ccactgcagg aaaagagagg
     2101 gtgcgcgtcc tgaccgacaa actcgccgag cacgagttcg cggtcggtta cttctacatg
     2161 cgaaaggggg cgccggccgc ggcgctcgtg cgcttcacgg agctcgagca gaggtatccc
     2221 gagtacgggg cgcgcgacaa gctcttcttc tattcggcac gggccctgga aaggctcggc
     2281 cgccgcgagg aggcgaaccg ctactactcc cggatcctcg aggagtttcc cgagagcgag
     2341 tgggcccgga gggcccgggg cgagcggggc ggaaaagccg ctccggccgc cgcggtcgct
     2401 gcgacgccga agtcctgagg ccaaagtccc gggcagcaag gatttccggc tgcttcgggc
     2461 ctcgttgttt ccagtcttga cccatgctgt ccgttgacag cccgcgggtg ggagcgtact
     2521 tttaagtcat aagtcgtgtc tccccgaatt tcttaaggag ccgccatgcg tctccccgct
     2581 cgtactgtca ctttcgttct ggccggtgta ctgacggccg gcgccggact tgctcaggtg
     2641 cgtgcccggc cggcgcaaac cgtcccccgg gagcctcaga aagagatccc cggaggggcg
     2701 atcgtcgtgg ccccgacgac ccagacggtc gtcaccgaag gagcggcttc ggtcgagacc
     2761 gcgccccagc cgctcggcta cgagagcgat ctccactgct tcggatacct cggagcgcca
     2821 cgggagacgt tcgttgccgg ggttgtcggc gccgagggag tggcggagca gatcgatttc
     2881 ttcacgggcg accttctcta cgtggacgcc ggctacaacc gtggcctcaa agtcggagac
     2941 gagttctggc tcgtgactcc cgagcagatc gtcgtccatc ccacgacggg taaagacctt
     3001 ggccgttttt accagtaccg cggccgggcc accgtcgaga ccctcgaagg gcggacggct
     3061 tcggtccggg tctcgagcgc ctgctcggac attccaatag ggtcgtatct gaagccgttc
     3121 gagccgatcc cgatcccact cgcccgaaag acccctccgg ccgtcgcagg agatccaccg
     3181 tccggaaagg cccggggtca catcgtgtac acgagagacg gagtcgtggc agtcggagcc
     3241 gaccacacga tcatcgtgga cctcggtgtc gccgatggaa tcgagccagg ggactttctg
     3301 acgatttacc gctacgccgt gggacgcgag tacggaatcc gtccggtcgg agcatactgg
     3361 gtcaatctgc ctccgcctcc cggggcggag attccccgca cgtatctcgg tgagatctcg
     3421 attctctccg tcggcgatcg ctgggcgatc gggcgcgtct cggattccta tcggctgatc
     3481 gaagtcggag acgaagtcga ggtgaagtag tcggttcggt tccgaacgac ttcaggagag
     3541 gcgcgatgcg cctcttcttt ttttcggcct ccggaattct caccgagtcg tttcgaacga
     3601 gtcgtcgagc tggcacgaaa atttctgcgg gtcctgcgca ccagctcgcg agttcagaat
     3661 tctttttgtc tcttctcgtc gcgagtcgcg tttgctaata tgcgcgcctc ctcgaacgct
     3721 ctcccgatct cggaggccga cgtgaacctg gattcgcaac ttcaacaggt catcgacgag
     3781 cttctctctc acggcatctc gctcgacctc gcgaagaagg aattcgagaa gaagtacatc
     3841 gtcggtgccg tgaagcgatc atcgggaaac ctcggacgcg cagcgtcctc tctcggcatt
     3901 caccgcaaca cgctgcggaa caaggtcggc atcctgaaca tccggttccc gcgcgatcag
     3961 taccgcgtca gcgtctcgcc ggacatccac aagaaaaagt agcaggtcag gtcttcacgg
     4021 acacggactc tccccgatgg gagagagtgg ccttgccatc ttcgcgaagc gaagtggcaa
     4081 gtggagacct gaccccctag cttggcggag ggttcttgcc gccgggctct tccggtttga
     4141 cggagtcctt gaagttcttg aggccctgcc cgagtccctt gccgagctgg gggaggcggg
     4201 aagccccgaa tatcaccaat acgatcaaca ggatgatgag aagctcgggg actcccaggc
     4261 gaccgaacat ttcagtttct cctcgccggc aagcttaaca gaggtgtccg agccgtgcct
     4321 cgaacacggt ccggaatcgg gtatccgcgg gcgcggtcag gagccgccgg acgaggccga
     4381 gctcgccagg caggtagcgc cggtaagtct cgccccgccc ctgcgagacc gcgcgcgcga
     4441 acgtgccgca gaccttccac gctcgctgaa ggagcacgcg ctccaaccgc cgggcgaatt
     4501 cctcggcacg gagtccgcga cgctcggcga actcccgcac gatcttctcc ccgtggtcgt
     4561 cgaccatcca atcgagcgtc gtcctctccc agagaagcga cgcgacgtcg taggcatcgg
     4621 gtccgctgcg cagatcctgg aaatcgatcg cagcgacgag gtcgccgacg gggaagagat
     4681 tgtttccgtg aaagtcgcga tgacagagca cgcgcggatg ttcctcgatc tcgcgcgtga
     4741 gcgacgccgc ccactcgtcg tgagccgccc ggtcgtcgga ggagagcggg gcaccgagcc
     4801 acaaatcgaa gactgcttcg cgcgcaaggt cgagctcgcg ccggaagaac gcggcgtcga
     4861 agggcgagtt gatgcgcgga tcgggcgcgg ccgcgattct ctccgcgacg tcgagggcgc
     4921 ggccgagcca gtcgctgcgc tccgacggtt gccccgcgag acgcgtcgcg aggtcttccg
     4981 gtccgaagtc ctcgaggatc tgactgcctc cccggtcctc cgcgtacacg ttcggcacgc
     5041 gaacgcccgc gccggacaga accgatcgtg ccgcaagcca tctccgcctc gcgtcctctt
     5101 cctcgggggg atagaagaca ccgaggacgg tcttgttgcc gccgacggtg agccgcagat
     5161 agcgacggcg cccgacgtct ccggggagaa cctcgaccgc gcacggctcg tagtgcgtgc
     5221 ggcgcgcgag ctcctggaga cccgcgaatt ccgcagggat caaggattca ccgggagaag
     5281 tcgggatcaa atgaaaaagg gatcagaggc acggcccctg atccctcgtc gggttcgctg
     5341 ccggtctcac gaaagcgatt tcgcctggga gagcaccacc ttc
//
LOCUS       DASWAV010000429         2771 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U2665
            k141_663207, whole genome shotgun sequence.
ACCESSION   DASWAV010000429 DASWAV010000000
VERSION     DASWAV010000429.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960316
            Sequence Read Archive: SRR10260007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2771)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2771)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:47:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,929
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2771
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_663207"
                     /isolate="SMAG_U2665"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.36444 N 132.31889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1096
                     /locus_tag="VF958_08920"
     CDS             <1..1096
                     /locus_tag="VF958_08920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009095595.1"
                     /note="This protein belongs to the uracil DNA glycosylase
                     superfamily, members of which act in excision repair of
                     DNA. However, it belongs more specifically to UdgX branch,
                     whose founding member was found to bind uracil in DNA
                     (where it does not belong), without cleaving it, appears
                     to promote DNA repair by a pathway involving RecA, rather
                     than base excision; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="UdgX family uracil-DNA binding protein"
                     /protein_id="HEX9149266.1"
                     /translation="PSNPLLDRTTAIVLPASGDNITLVRDVVIQPTLESWRAAARTLL
                     AENVAPDQIVWREAAKQGGLFDAVVVAEPAGASRGADFRVPREFVEIARLAARHTDPE
                     RWRLLYSVLFRVARGGAPDLLASEGDPDVVRLKRIAKHIADADLAARTPGARQFVPKD
                     ANSLEELRAAAASCQGCDLYRDATQTVFGKGPQTARAILVGECPGDQEDIQGAPFVGP
                     AGDVLSRALVDADLPRADVYVTNVVKHFKFIRTPKRRLHQTPGTVEIGACRPWLEAEL
                     ALIRPEMLVCLGGTAAKLLLGPDFRIMKQRGQIMTGTGWAPKAMATLHPSAVLRAEDS
                     AGQERLYGMLVSDLRLVRREVHLEDLRVGT"
     gene            1093..2499
                     /locus_tag="VF958_08925"
     CDS             1093..2499
                     /locus_tag="VF958_08925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012252513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HEX9149267.1"
                     /translation="MNSESPPGPPGRLLLGNLPDFARDVLGFLTFCAREYGDVTRMRL
                     GRYDAYLFNHPDLVDEVLRKQRENFIKHRFFWRHVTAIFGTGLLTSEGDFWLHQRRLA
                     APAFHPDRIANYGDVMVSYTDRLVDGWRDGETRNVHEDMMRVTMEIVSKTLFDADVET
                     DVDELGRAFEIVLREIAARFRRPFKIPDWIPTPGNLRYGRGIRRLERLVFRILAERRA
                     DPHDRGDLLSMLMSARDDEGNAMSDSQLRDELVTIFLAGHETTAIALSWTWHLLAQHP
                     LVKDRLVKELFDQLGRRPATVHDLPNLPFADAVVKEAIRLYPPAYVLGREALADCEIG
                     GWRIPAGATVFVSPWVMHRDRRWFASPEKFLPERWLDGLGARLPRFAYIPFGGGPRVC
                     IGDRFATMEAVLILATVARRDRLERTSAAEIGPFPSITLRPEGGVRMKLSRRQPMTTG
                     TAQAAEDDLRETPGAGSA"
     gene            complement(2491..>2771)
                     /locus_tag="VF958_08930"
     CDS             complement(2491..>2771)
                     /locus_tag="VF958_08930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011955044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="haloacid dehalogenase"
                     /protein_id="HEX9149268.1"
                     /translation="TRRHFKVSFDVVVTAQQVRSYKPAPGHFEEARRRVGEACWLHAA
                     QSFFHDVVPARNLGIPVAWVNRKAERASGVIRPDLEVRNLTELAGALR"
BASE COUNT          427 a          979 c          906 g          459 t
ORIGIN      
        1 cccatcgaat ccgctgctcg accggacaac cgccatcgtt cttccagcct ccggcgataa
       61 catcacgctc gtgcgggacg tcgtcatcca gccgacgctc gagagctggc gcgccgcggc
      121 aagaaccctg ctcgcggaga acgtcgcgcc ggatcagatc gtctggcgcg aagcggcgaa
      181 gcaaggcggc ctcttcgacg cggtcgtggt cgccgagccc gccggcgctt ccaggggcgc
      241 cgactttcgc gtcccgcgcg aattcgtcga gatcgcgcgc ctcgcggccc ggcacaccga
      301 cccggagcgc tggcgcctcc tctactcggt tctgtttcgc gtcgcgcgag gcggcgcgcc
      361 cgacctcctc gcgtcggagg gggatcccga cgtcgtccgc ctgaagcgca tcgcgaagca
      421 catcgccgat gccgacctcg cggcccgtac tcccggtgct cgccagttcg tcccgaagga
      481 tgcgaacagt ctcgaggagc ttcgcgcggc cgctgcgtcc tgtcagggct gtgatctcta
      541 ccgcgacgcg acgcagaccg tcttcggcaa aggtccgcag acggcgcgcg cgattctggt
      601 cggcgagtgt cccggcgacc aggaggacat ccagggcgct cctttcgtcg ggcccgccgg
      661 cgatgtcttg agtcgggctc tcgtcgacgc cgatctccca cgcgccgacg tgtacgtgac
      721 gaacgtggtg aagcacttca aattcattcg aacgcccaag cgccgcctcc accagacgcc
      781 gggcacggtc gagatcgggg cgtgtcggcc ctggctggag gccgagctcg cattgatccg
      841 gccggagatg ctcgtctgtc tgggcggcac cgccgcgaag ctgctcctcg gtcccgactt
      901 ccgcatcatg aaacagcgcg gccagatcat gaccggcacc ggctgggctc cgaaggcgat
      961 ggcgacgctc cacccttccg cggtcctccg cgccgaggat tccgccgggc aggagcggct
     1021 ctacggaatg ctcgtttccg acctccggct cgtccggcgg gaggtacact tggaggatct
     1081 tcgtgtcgga acgtgaattc cgaaagccct cccggccccc ccggacgact cctcctcggt
     1141 aacctcccgg acttcgcccg agacgttctc ggcttcctga ccttctgtgc gcgcgagtac
     1201 ggagacgtca cgcgcatgcg tctcgggcga tacgacgcgt acctcttcaa ccaccccgat
     1261 ctcgtcgacg aggtcctccg caagcagcgc gagaacttca tcaagcatcg cttcttctgg
     1321 cggcacgtga ccgcgatctt cggaactgga ctcctgacga gcgaagggga cttctggctg
     1381 caccagcggc gcctcgccgc gccggcgttt cacccggatc gaatcgcgaa ctacggcgac
     1441 gtgatggtgt cgtacacgga ccgtctggtc gacggctggc gcgacggcga gacgcggaat
     1501 gtccacgaag acatgatgcg ggtgacgatg gagatcgtct cgaagactct cttcgacgcg
     1561 gacgtcgaga ccgacgtgga cgagctcgga cgcgctttcg agatcgtgct ccgcgagatc
     1621 gcggcgcgat ttcgccgacc gttcaagatc cccgactgga tcccgacgcc gggcaacctg
     1681 cgttacggcc gcggcatccg gcggctcgag cggctcgtct ttcggatcct cgccgaacgg
     1741 cgcgccgatc cgcacgaccg cggcgacctt ctctcgatgc tcatgagcgc gcgggacgac
     1801 gagggcaacg cgatgtcgga ttcgcagctg cgcgacgagc tcgtcacgat cttcctcgcg
     1861 ggtcacgaga cgaccgcgat cgctctctcc tggacctggc atctgctcgc gcaacacccg
     1921 ctcgtgaagg accggctagt caaagagctg ttcgaccagc tcggccgacg tccggcgacc
     1981 gttcacgacc tcccgaacct ccccttcgcg gatgccgtcg tgaaggaagc gatccggctc
     2041 tatccgcctg cctacgtcct ggggcgcgag gcgctcgcgg actgcgagat cggcggctgg
     2101 agaatccccg ccggcgcgac ggtgttcgtg agcccctggg tgatgcaccg tgaccggcgg
     2161 tggttcgcga gccccgagaa gttccttccc gagcgctggc tcgacggcct cggggcgcgt
     2221 ctcccccgat ttgcgtacat cccgttcggc ggcgggccgc gcgtgtgcat cggggaccgc
     2281 ttcgcgacga tggaggccgt gctcatcctc gcgaccgtcg cgcgccgcga tcgtctcgag
     2341 aggacctcgg ctgcggagat cggtcccttc ccctcgatca cgctgcgacc ggaaggtggc
     2401 gtccggatga agctgtcgcg gcggcagccg atgacgacag gcaccgcgca ggcggccgag
     2461 gacgatctga gagagacgcc cggcgccgga tcagcgtagc gcgcccgcca gctcagtcag
     2521 gtttcgaacc tcgaggtcgg gccggatgac tcccgacgcc ctttcggcct ttcggttcac
     2581 ccacgcgacg ggaatcccga ggttccgcgc cggcacgacg tcgtggaaga agctctgcgc
     2641 tgcgtgcagc cagcacgctt cgccgacgcg gcgccgcgcc tcctcgaaat gtccgggtgc
     2701 gggcttgtac gagcggacct gctgcgcagt gacgacgacg tcgaacgaaa ccttgaaatg
     2761 ccgccgcgtt g
//
LOCUS       DASWAV010000430         5407 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U2665
            k141_184420, whole genome shotgun sequence.
ACCESSION   DASWAV010000430 DASWAV010000000
VERSION     DASWAV010000430.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960316
            Sequence Read Archive: SRR10260007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 5407)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5407)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:47:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,929
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5407
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_184420"
                     /isolate="SMAG_U2665"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.36444 N 132.31889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(274..696)
                     /locus_tag="VF958_08935"
     CDS             complement(274..696)
                     /locus_tag="VF958_08935"
                     /inference="COORDINATES: protein motif:HMM:NF025852.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEX9149269.1"
                     /translation="MAQGGAAAKKFTVWPAADLKWQPLAGAPGPAGAPGPMLAVISGD
                     PTKGAYTAIEKFPAGFSAPMHTHPATHKAVIISGTWIHGEPGKPDVRLGPGSYLVQPA
                     GMKHTTACDAASECVFYIESSGKFGITLVDEKKAAAKK"
     gene            944..1726
                     /locus_tag="VF958_08940"
     CDS             944..1726
                     /locus_tag="VF958_08940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015348863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX9149270.1"
                     /translation="MRSLVRGLLPISGIAVLVVLWDVALRLPGSSRLLPRPLAVVRAI
                     GELAQKGFLVKHVVASLFRVTWGYVGAVLVGVPLGIVLGWYRRGGMAIAPLLEVLRPI
                     SPLAWIPIAILWFGVGDVSAVFIIFLGALFPITLTAMNAVRGVDLVHVNAGRNFGLRA
                     PELLRRVIYPAILPQLLVGLRLSLGIAWLVVVAAEMIAVSSGLGFLIIDARNAGNRYD
                     LVVAGMVLIGVIGIALDALIRGLERSKALAWGYTAIEDAANP"
     gene            1723..2487
                     /locus_tag="VF958_08945"
     CDS             1723..2487
                     /locus_tag="VF958_08945"
                     /EC_number="7.6.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007495183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX9149271.1"
                     /translation="MTGSASVKVRILGLSKTFQTNGNEIRVLDNIDLDVREGELVCIL
                     GPSGCGKSTLLNIVGGFLPASSGEVAIDGAPVREPDPRRIFVFQERGVFPWLTVEGNI
                     GFGINGLSPAEKTERIAHYVRLVGLQGFENAYPQELSGGMKQRVEVARALAVNPDVLY
                     LDEPFGALDSITRLVMRSELLRIWEAEKKTILFVTHDIEESCQIADRVVVLSARPGSI
                     RRIVDVDLPHPRDLSSPRYLALRDSILAEIGLAHRI"
     gene            2484..3314
                     /locus_tag="VF958_08950"
     CDS             2484..3314
                     /locus_tag="VF958_08950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007644421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX9149272.1"
                     /translation="MNTRRKTENEERRTDDGPPLQQSPGVLEAVLLPILSAAVLIAVW
                     QAVVVLSHTQIFPSPLAVVRGIAELSRKGVLGIYVRDSLLRVGAGYFLALLCGIPLGI
                     ALGWYSAAARAINPVIQFLRPISPLAWIPVAIVLFGVSNAAAIFLIFLASFFPIVVAA
                     MNGVRNVPAMYLQAGRNFGLSTPALFSRVIFPAALPRVLTGLRVSLGVAWIVVVAAEM
                     IAVNSGLGYLVIDSRNAGKRYDLVVAAMLLIGVIGLALNTLVRQVERLRAVRWGFNAP
                     "
     gene            3304..4371
                     /locus_tag="VF958_08955"
     CDS             3304..4371
                     /locus_tag="VF958_08955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020721103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX9149273.1"
                     /translation="MLRSRTARNLVLGLAVWIVALTAAHFALNVDWSSVVNDRLPLAK
                     RKLNVAYIPVTCHLTCPVTDFISRYSLDGNIFIPRMFQGFPEIKEALLSDRMQVGFMV
                     APMAIALAAQGAPIKIVYLGHRYGSAVVVRKDGPIHSVEDLAGHTIAIPSRFSDERLI
                     VFKALAQHGVAAASVKMIEMSPPDVAGALAAGAIDAFSMGEPYPSQAELGGFGRVLFQ
                     AREYWPDYMSCVVVARQDVIDRRPKALQVLVDGIARSGLWLDEGRPHREHAADFVARY
                     YYNQPPELLRHALTEPLDRVIYTRLSPRKPDFDMVRDLMIQTGVLDRKIDFDEYVDVR
                     FAEGAVGRTSWKYEPGSGKAE"
     gene            complement(4392..5318)
                     /locus_tag="VF958_08960"
     CDS             complement(4392..5318)
                     /locus_tag="VF958_08960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9149274.1"
                     /translation="MIRAIVVAGGVLCASLVSPAAPTESGWTLDQVGTRLTVLSGVWG
                     TSPQNVFAVGGRGVILHFDGSRWRHQESGTGKDLAAVWTGSRTDAFAVGLDGVILHFD
                     GRGWEKEKSGTSKTLLAVWGASPKDVYAVGRGGTILHFDGRSWTRQPSPTRYDLTAVW
                     GSSASDVHAAGDHGTILHSDGTRWTQPAASFGFHWRFAALGGTSAGNVYAGGSRRPSG
                     QLRDEVLEGVVLRYDGKAWKPVAGGEAQVVGGIWAVSPDEIYFTGPAINGLEEVRRYD
                     GAEFRIVDSESRFSPRARRAARSSSRERTGSS"
BASE COUNT          791 a         1908 c         1719 g          989 t
ORIGIN      
        1 gagcccgatc actcgtcgct ctcgctcgct cgggatccgc cgaaggcacc ggcgctcagc
       61 tcggcgtgca tgcctcgacc gagcgcctga tccctcgctc actcccgctc ccgcaacgtg
      121 aggctcctcc cttcgggaga ctcctcacgc tgcgaccaga gcccgatcac tcgtcgctct
      181 cgctcgctcg ggatccgccg aaggcaccgg cgctcagctc ggcgtgcatg cctcgaccga
      241 gcgcctgatc cctcactgcg gtcgagcgac cggttacttc ttggcggccg ccttcttctc
      301 gtccaccagc gtgatgccga acttgccgct gctctcgatg tagaacacgc actccgacgc
      361 ggcgtcgcat gcggtcgtgt gcttcatgcc cgccggctgg accagatacg acccggggcc
      421 aagccgcacg tcgggttttc ccggctcacc gtggatccag gtgcccgaga tgatcacggc
      481 cttgtgagtg gcgggatggg tgtgcatcgg agcggaaaac ccggccggga acttctcgat
      541 cgccgtgtat gcgcccttcg tcgggtcgcc cgagatcacc gccagcatcg ggccgggcgc
      601 ccccgccggg cccggcgctc ccgccagggg ctgccacttc agatcggccg cgggccacac
      661 cgtgaacttc ttcgccgccg ctccgccctg cgccatcaga actccagccg ctagaaagag
      721 agccgaatgg accagggctt tcctcgaggt cttcgacatg aggttcctcc ttcgtttgtc
      781 gttcgcttcg aagccgcgaa atcctatccg agcgccgcat gacgggaccg tgaacgggtc
      841 cgcgggtgag cgttcggatc cacggctttg ctagactccc gcggctttgg cgcccccggt
      901 cccaacggaa gccccttccc ccgacagccg gcgcgcgcgg ccggtgcgct cgctcgtccg
      961 ggggcttctc ccgatctccg gaatcgcggt cctcgtggtc ctctgggacg ttgccctgcg
     1021 tctgcctggc tcctcgaggc tcctgccacg cccgctcgcc gtcgtccgcg cgatcgggga
     1081 gctcgcgcag aagggcttcc tcgtcaagca cgtcgtcgca tcgctcttcc gggtgacatg
     1141 gggctacgtc ggcgcggtcc tcgtgggcgt cccgctcggc atcgtgctcg gctggtatcg
     1201 ccgcgggggc atggcgatcg cgccgctcct cgaagtcctc cgaccgatct cgcccctcgc
     1261 ctggatcccg atcgcgatcc tctggttcgg cgtcggcgac gtttccgccg tcttcatcat
     1321 ttttctcggg gcgctttttc cgatcacgct gacggcgatg aacgcggtcc gcggcgtcga
     1381 cctcgtgcac gtcaacgcgg gccggaactt cgggctccgg gcgccggagt tgctgcggcg
     1441 ggtcatctac cccgcgatcc tgccgcagct tctcgtgggg ctgcgcctct cgctcggaat
     1501 cgcttggctc gtcgtcgtcg cggcggaaat gatcgcggtc agctcgggac tcggctttct
     1561 catcatcgac gcgagaaacg cgggcaaccg ctacgacctc gtcgtggccg ggatggtcct
     1621 gatcggcgtt atcgggatcg cgctcgacgc cctgatccgc ggtctcgagc ggtcgaaggc
     1681 cctggcctgg ggatacacgg cgatcgagga cgcagcaaat ccatgacggg atctgcgtcg
     1741 gtcaaggtcc gcatcctggg actctcgaag acgttccaga cgaacggcaa cgagatccgc
     1801 gtactcgaca acatcgacct cgacgtccgc gagggcgagc tggtgtgcat cctcggtcct
     1861 tcgggctgcg gcaagtccac gcttttgaac atcgtcggcg gctttctccc ggcgtcgagc
     1921 ggagaagtcg cgattgacgg cgcgccggtc cgcgagcccg acccccgccg gatcttcgtt
     1981 ttccaggagc gaggcgtgtt cccgtggctg acggtcgagg gcaacatcgg cttcggcatc
     2041 aacggccttt cgccggcgga gaagaccgag cggatcgccc actacgtccg cctcgtcggt
     2101 ctccagggat tcgagaacgc atacccgcag gaactctccg gcggcatgaa gcagcgcgtc
     2161 gaagtggcgc gcgcgctcgc cgtgaacccg gacgtgctct atctcgacga gcccttcggc
     2221 gcgctcgact cgatcacccg cctcgtgatg aggagcgagc tccttcggat ctgggaggcg
     2281 gagaagaaga cgatcctctt cgtgacccac gacatcgagg agtcgtgcca gatcgcggac
     2341 cgcgtcgtcg tgctctccgc gcgccccggc tcgatccgcc ggatcgtcga cgtcgatctc
     2401 cctcacccgc gcgacctgtc ctcgccgcgc tatctcgcgc tgcgcgacag catcctcgcg
     2461 gagatcgggc tcgcccatcg gatatgaaca cgagacggaa aacggaaaac gaggaacgaa
     2521 gaacggacga cggtcccccg cttcagcaaa gcccgggcgt tctcgaggcg gtcctgctgc
     2581 cgattctgtc ggcggccgtc ctgatcgcgg tctggcaggc ggtcgtcgtt ctctcgcaca
     2641 cgcagatctt tccttccccc cttgccgtcg tgcgggggat cgccgagctt tcgcgcaagg
     2701 gggtgctcgg tatctatgtt cgcgactccc tcctccgtgt cggcgcgggt tattttctcg
     2761 cccttctctg cggcatcccg ctcggtattg ccctcggctg gtactccgcg gcagctcgcg
     2821 caatcaatcc ggtaatccag ttcctccgcc cgatcagccc gctcgcgtgg atccccgtgg
     2881 cgatcgtgct gttcggggtc agcaatgccg cggcgatctt cctgatcttc ctcgcttcgt
     2941 tcttcccgat cgtggtcgcg gccatgaatg gcgtgcgtaa cgtgcccgcg atgtatctcc
     3001 aggcggggcg gaacttcgga ctgtccacgc cggcgctctt ttcccgggtc atcttcccgg
     3061 ccgcgctgcc ccgggtcctg accggccttc gcgtctcact cggcgtcgcc tggatcgtgg
     3121 tcgtcgccgc ggaaatgatc gccgtcaatt cgggtctcgg ctatctcgtg atcgactcgc
     3181 gcaacgcagg caagcgctac gacctcgtcg tcgcggcgat gctgctgatc ggggtgatcg
     3241 gcctcgcgct caacacgctt gtgcgtcagg tcgagcggct gcgcgcggtg cgctggggct
     3301 tcaatgctcc gtagccgcac agcgagaaac ctcgtcctcg gcctcgcggt ctggatcgtc
     3361 gctctcaccg cggctcactt cgcgctgaac gtcgactggt cgagcgtcgt gaacgatcgt
     3421 ctgccgctcg cgaagcggaa gctgaatgtc gcctatatcc cggtgacctg ccacctgacg
     3481 tgtccggtga cggacttcat ctcgcgctac tccctcgacg gaaacatctt cattccgcgg
     3541 atgttccagg gtttccccga gatcaaggag gcgctcctct ccgatcggat gcaggtcggg
     3601 ttcatggtcg ccccgatggc gatcgcgctc gccgcgcagg gcgcgccgat caagatcgtc
     3661 tacctcggtc atcgttacgg gagcgccgtc gtcgtccgga aggacggccc catccattcc
     3721 gtcgaagatc tcgccggcca caccatcgcg atcccgagcc gcttctcgga cgagcggctg
     3781 atcgtcttca aagcgctcgc gcagcacggc gttgccgcgg cgtcggtcaa gatgatcgag
     3841 atgtcgccgc ccgacgtcgc gggagcgctc gccgccggcg cgatcgacgc gttctcgatg
     3901 ggagagccct atccctcgca ggccgagctg ggcggtttcg gccgcgtcct ctttcaggcc
     3961 cgcgagtact ggccggacta catgtcgtgc gtcgtcgtcg cgaggcagga cgtcatcgac
     4021 cggcgaccca aagcgcttca ggtcctcgtg gacggaatcg cgcggtcggg cctgtggttg
     4081 gacgaaggtc gcccgcaccg ggagcacgcg gccgatttcg tcgcgcggta ctactacaac
     4141 cagccgcccg aactcctgcg gcacgcgctc acggagccgc tcgatcgggt catctacacg
     4201 cgcctctcgc cgcgcaagcc cgacttcgac atggtgcggg acctgatgat ccagacgggg
     4261 gtcctcgacc gcaagatcga tttcgacgag tacgtcgacg tccgcttcgc ggaaggagcg
     4321 gtcggccgta cgtcctggaa atacgagccg gggagcggga aggctgagta ggcgtcagcg
     4381 cgacgtcttc gtcaggacga acccgttcgt tcccgcgacg aagacctcgc cgctcggcgc
     4441 gcccggggag agaagcggct ctccgaatcg acgatccgga actctgcccc gtcatatcgc
     4501 cggacctcct cgagaccgtt gatcgcggga ccggtgaaat aaatctcgtc gggcgagacg
     4561 gcccagattc ctcccacgac ttgcgcctcg ccgccggcga ccggcttcca cgccttgccg
     4621 tcgtacctca ggacgacgcc ctcgaggact tcgtcccgca gctgaccgct cggtcgccgc
     4681 gagccgcccg cgtagacgtt gccggcggag gttcctccga gcgccgcgaa ccgccagtga
     4741 aagccgaatg aggcagccgg ctgcgtccaa cgcgttccgt cggagtgcag gatcgttccg
     4801 tggtcgccgg ccgcgtggac gtcgctcgcg gacgatcccc agaccgcggt caggtcgtac
     4861 cgtgtgggac tcggctgccg agtccaggat ctcccatcga agtggaggat cgtccctcct
     4921 cggccgaccg catagacgtc cttgggggac gccccccaga cggcgaggag agtctttgaa
     4981 gtcccgctct tctccttttc ccagccgcgg ccgtcgaagt gaagaatcac gccatccagt
     5041 ccgaccgcga acgcatcggt ccgcgagccg gtccacaccg ccgcgaggtc cttcccggta
     5101 ccactttcct ggtgcctcca ccgggagccg tcgaagtgca ggatgacgcc gcggccgccg
     5161 accgcgaaaa cgttctgcgg cgacgttccc catacgccgg aaagaaccgt cagccgggta
     5221 ccgacctggt cgagcgtcca cccgctctcg gtgggagcgg ccggagagac gagcgaggcg
     5281 cagaggacgc cgcccgcgac gacgatcgcg cggatcacga gcgcgcgtcg cgcagcttct
     5341 tcagctcggt gatgtcgcgg ttgacgaaaa tcgcggcaag cggccggccg aattcgtcgc
     5401 cgagcga
//
LOCUS       DASWAV010000431         5552 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U2665
            k141_381596, whole genome shotgun sequence.
ACCESSION   DASWAV010000431 DASWAV010000000
VERSION     DASWAV010000431.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960316
            Sequence Read Archive: SRR10260007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 5552)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5552)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:47:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,929
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5552
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_381596"
                     /isolate="SMAG_U2665"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.36444 N 132.31889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(48..1280)
                     /locus_tag="VF958_08965"
     CDS             complement(48..1280)
                     /locus_tag="VF958_08965"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF045194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX9149275.1"
                     /translation="MFSRASAGVRRLVRELLPAPLRRSYHRFALLRDFGIEPRTGRRR
                     PSVLDGALPRGLNVVGWFDSPTGIGQSVRSLARSAEAAGLPVERIEASALGSGARVSA
                     PYALNLFHVNADGAGSIVELCGPAVHRGRANVGYWYWETEVFPAIWSDRFAYFDEIWV
                     ASEFCRAAIAASSDIPVAVVPPPVIFDDLPARETAPSTGVPFRFVTICDAESVAERKN
                     PLGAVRAFARAFPEDSAVALTVKIANAGRAPALVESIANAAGSAHVEIDTSALARGDI
                     ERLLSGCDAYVSLHRAEGFGLPIAEAMALGKPVVATGYSGPLDFLDETTGYPVRWAPA
                     VLDRARGPYPAGTRWAEPDEAHAVETLRGVVSDRSDSVRRGQAAKRRMESLYGLEAAG
                     RRLAEHADRLLARLAAQP"
     gene            complement(1264..2403)
                     /gene="wecB"
                     /locus_tag="VF958_08970"
     CDS             complement(1264..2403)
                     /gene="wecB"
                     /locus_tag="VF958_08970"
                     /EC_number="5.1.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016348778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008761 - UDP-N-acetylglucosamine
                     2-epimerase activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (non-hydrolyzing)"
                     /protein_id="HEX9149276.1"
                     /translation="MRSPRRILVIVGTRPEAVKLAPVIRRLRQEPARFETIVCVTAQH
                     RQMLDQVLELFEIAPDIDLDLMRDDQASNELASRVFAALDSVLVDRAPDWLLVQGDTT
                     TAMCAAVAAFHRRVRVGHVEAGLRTGDLREPFPEEMNRRVVDMVADAYFAPTERAAAA
                     LRRELVPEERIHRTGNTVVDALVQIAEKQGRVELEDLVLVTAHRRESFGEGLAAIVAA
                     VARLARSFPRFRFVHVVHPNPNVRAAISGYGKLPNVEIVEPLDYGSLVSLMRRSRLIL
                     TDSGGIQEEAPTFGKPVLVLRRKTERPEGIEAGLARLVGTDQDRIVAEAELVLSGRSD
                     LACVASRANPYGDGRASDRIAAALDESPYEPFEPALSGTAVVQPR"
     gene            2472..3467
                     /locus_tag="VF958_08975"
     CDS             2472..3467
                     /locus_tag="VF958_08975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005994207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX9149277.1"
                     /translation="MTARMPSLTGKNILITGGLGFIGSNLAIRLVRDGARVAVCDAMI
                     EGYGGNFENVRDVQSSLEIHLSDVRDETEMSRLVEGRDVVFHLAAQVSHVLSLADPYP
                     DIDINIRGTAAVLEACRKRNPGALVVRSGTRGQYGPAVRLPVSEDAPADPRGIYEISQ
                     LSAEMICRTYTRIHGVRTVPLRLTNVYGPRAQMKHSHFGVVNWFVRLALEGKPIPVFG
                     TGRILRDFLYVDDCVEALVAVACEPAAVGEIINVGNDRSSTFLDVAEILRDLVPGAEI
                     VFTDFTPERRAQEPGDFVSDISKIRRLTGWSPEVELREGLARTVEFYRERRADYF"
     gene            3624..4190
                     /locus_tag="VF958_08980"
     CDS             3624..4190
                     /locus_tag="VF958_08980"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017815618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HEX9149278.1"
                     /translation="MASIHPTALVASRSIGEGTRIWAFVNVLEGATIGRDCNICDRCF
                     IENDVVLGDRVTVKCGVSLYDGVVLEDDVFVGPDVSFSNDVRPRSRQHRNSHPKTLVR
                     TGASLGAGTILLPGVTVGRWAMVGAGSLVTRDVPDFALVYGSPARVHGSVCRCGESLR
                     LVDRRADCSCGRRYELQPEGGVAETPVP"
     gene            4187..5323
                     /locus_tag="VF958_08985"
     CDS             4187..5323
                     /locus_tag="VF958_08985"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012257104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HEX9149279.1"
                     /translation="MKATAAPSSARVPFGSLDADYRERRGAIDAAIARVLSRGRFILG
                     EEVESFERELAAYLDVRHAVACASGTDAIAIALAASGAGSEDEVLLPANACVPVIAGV
                     RLAGAQPRLADVERATLTLSRAAAERALGPRTRFLLAVHLYGGLADVEGLRALARDRS
                     LVLVEDCAQSLGALADGRRAGSFGSASAFSFYPTKNLGAYGDAGSVATSDDSIAARAR
                     LLRQYGWTRRDFSEVEGRNSRMDELQAAILRAKLPYLEADNARRREIVRRYDKGLADL
                     PLRRPAALARTTPAPHLYPVLVSSREALREHLAGRGIETGIHYPVPIHLQPAYAFLGH
                     SRGDFPVSEEASETVLSLPLYPTLADDQVEAVISAMRDFFGRPR"
     gene            5320..>5552
                     /locus_tag="VF958_08990"
     CDS             5320..>5552
                     /locus_tag="VF958_08990"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX9149280.1"
                     /translation="MSGERISVVVPVYFNAESLPRLADRLRELADRADFTLEPIFVDD
                     GSGDDSWPRVQAIVAAWPVARGIRLTRNFGSQMA"
BASE COUNT          846 a         1953 c         1882 g          871 t
ORIGIN      
        1 cccgagcgcg tagagtctcc cgcggatccc cctctcccag tcgggtctca tggctgcgcg
       61 gcgagccggg cgagaagccg gtccgcgtgt tcggcgagac ggcggcccgc ggcttcgagc
      121 ccgtagagac tctccatccg acgcttcgca gcctgaccgc ggcggaccga atcggagcga
      181 tccgagacga ccccgcgcaa cgtctcgacg gcgtgcgcct cgtccggctc cgcccagcgg
      241 gttccagcgg ggtaaggccc gcgcgcccga tcgagcaccg ccggtgccca tcgcaccggg
      301 tagccggtcg tctcgtcgag aaagtcgagc gggcccgagt atcccgtcgc aacgacgggc
      361 tttccgagag ccatcgcctc ggcgatgggc aggccgaatc cctccgcgcg atggagcgag
      421 acgtaagcgt cgcatccgga gaggaggcgt tcgatgtcgc cgcgtgcgag ggccgacgtg
      481 tcgatctcga cgtgcgcgct ccccgcggcg ttcgcgatcg actcgacgag ggccggtgcc
      541 ctcccggcgt tggcaatctt caccgtcagg gcgaccgccg agtcttccgg aaacgcccgg
      601 gcgaaggcgc ggaccgcacc gagcggattc tttcgctcgg cgacgctctc ggcgtcgcag
      661 atcgtcacaa agcggaacgg gacgcccgtt gaaggcgcgg tctcgcgcgc cgggagatcg
      721 tcgaaaatca ccgggggcgg cacgacggcg accggtatgt cggagctcgc ggcgatcgcg
      781 gcccgacaga actccgaggc gacccagatt tcgtcgaagt acgcgaaacg gtcgctccag
      841 atcgccggga agacttccgt ttcccagtac cagtagccga cgttcgcgcg gccgcgatgg
      901 accgccggcc cgcagagctc gacgatcgaa ccggcgccgt cggcgttcac atggaagaga
      961 ttcaaagcgt agggcgcgga gacgcgcgcg ccggacccga gggccgacgc ttcgatcctc
     1021 tcgaccggaa gccccgcggc ctccgccgac cgcgcgagtg agcgcaccga ctggccgatc
     1081 cccgtggggg aatcgaacca gcccacgacg ttcaaccccc gcggaagcgc tccgtcgaga
     1141 acggaagggc ggcgtcggcc cgttcgcggc tcgatcccga agtcgcgcag cagcgcgaac
     1201 cggtggtagc tccgtcggag cggcgcggga agcagctcgc ggacgagccg ccgaacgccg
     1261 gcgctagcgc ggctgaacaa cggcggtgcc ggacaacgcc ggctcgaagg gctcgtacgg
     1321 ggactcgtcg agagctgccg cgatgcggtc ggatgcgcgc ccgtccccgt aggggttcgc
     1381 gcgggaggcg acacacgcga ggtccgaccg tccggagagg acgagttccg cctccgcaac
     1441 gatccggtct tggtcggtgc cgacaagccg tgcgagcccg gcctcgattc cctcggggcg
     1501 ctccgtcttg cggcgcagga cgaggaccgg cttcccgaag gtcggcgcct cctcctggat
     1561 cccgccggaa tccgtcagga tcaggcggct cctccgcatc aacgagacga gcgatccgta
     1621 gtcgaggggt tcgacgatct cgacgttcgg cagcttcccg tatcccgaga tcgcggcccg
     1681 gacattcgga ttgggatgga cgacgtggac gaaccggaat cgcgggaacg accgcgcgag
     1741 ccgggcgact gcggcgacga tcgccgccag cccctcgccg aagctctctc gccggtgcgc
     1801 cgtgacgagg acgaggtcct ccaactccac cctcccctgc ttttccgcga tctggacgag
     1861 tgcatcgacg accgtgttgc cagtgcggtg aatccgctcc tccgggacga gctcccgccg
     1921 gagcgccgcc gccgcccgtt ccgtcggagc gaaataggcg tcggcgacca tgtcgacgac
     1981 gcgtcggttc atctcctccg gaaagggctc tcgaaggtcg cccgtccgaa gacctgcctc
     2041 cacgtgaccg acccgaacgc ggcggtgaaa tgccgcgacc gccgcgcaca tcgcggtcgt
     2101 cgtgtccccc tggacgagga gccaatcggg cgcgcgatcg acgagcacgg aatcgagggc
     2161 cgcgaacacc cgcgacgcga gctcgttgct cgcctggtcg tcgcgcatca ggtcgaggtc
     2221 gatgtccgga gcgatctcga agagctcaag gacctgatcg agcatctggc gatgctgtgc
     2281 ggtgacacac acgatcgtct cgaagcgcgc gggctcctgc cggagacgtc ggatgacggg
     2341 tgcgagcttg accgcctccg gccgggtccc gacgatgacc aggattcggc gcggtgacct
     2401 catgccgtct gacggggtcg tgtcgaagct cctctttcca tggggatcgt caaaccgatc
     2461 ccgatgctat cgtgaccgcg cgcatgcctt ccctcacggg gaaaaacatc ctgatcacgg
     2521 gcggcctcgg tttcatcggc tcgaacctgg ccatccgcct cgtgcgcgac ggcgcgcgcg
     2581 tcgccgtgtg cgacgcaatg atcgagggct acggcggcaa cttcgagaac gtccgcgacg
     2641 tgcagtcgtc cctcgagatc cacctgtccg acgtccggga cgagaccgag atgtcacggc
     2701 tcgtcgaagg ccgggacgtc gtcttccacc tggcggcgca ggtctctcac gtcctttcgc
     2761 tggcggaccc ctatcccgac atcgacatca atatccgggg aaccgccgcc gtcctcgagg
     2821 cgtgccgcaa acgcaacccc ggcgcgctcg tcgtccgttc cgggacgcgc ggccagtacg
     2881 gtccggcggt ccgcctgccg gtatcggagg atgcgccggc cgatccccgg ggcatctacg
     2941 agatctcgca gttgtccgcc gagatgatct gtcgaaccta cacccggatt cacggcgtga
     3001 ggacggttcc tcttcgcctg acgaacgtct acgggccacg cgcccagatg aagcactccc
     3061 acttcggcgt cgtgaactgg ttcgttcggc tcgcgctcga gggaaagccg atccccgtct
     3121 tcgggaccgg ccggatcctg cgcgacttcc tctacgtgga cgattgcgtc gaggcgctcg
     3181 ttgccgtcgc ctgcgagccg gcggcggtcg gcgagatcat caacgtcggc aacgaccggt
     3241 cgtcgacctt cctggacgtg gcggagattc tccgagacct cgttcccgga gccgagatcg
     3301 tcttcaccga tttcacaccg gaacgacggg cccaggagcc cggagatttc gtctcggaca
     3361 tatcgaagat ccgccggctg accggctggt cgccggaggt cgagctccgc gaaggcctgg
     3421 cgcggacggt ggagttctac cgcgagcggc gcgcggatta cttttgatcg attccggggt
     3481 caagtctcca cttgccacat tgggggtcag gtctccactt gccacttcgc gatgcgaagt
     3541 ggcaaggtcg ggaatccatc gccatctccg cggcgactcg tgaatacctg accccatcgg
     3601 ttaagctctt ccacaagcac tgcatggctt cgattcatcc gaccgcgctc gtggcgtctc
     3661 gttcgatcgg cgaggggacc cgcatctggg ccttcgtcaa cgtgctcgaa ggcgccacga
     3721 tcggacgcga ctgcaacatc tgcgaccgat gtttcatcga gaacgacgtc gtgctgggag
     3781 atcgcgtcac cgtcaagtgc ggcgtctcgc tctacgacgg ggtcgtgctc gaagacgacg
     3841 tcttcgttgg gcccgacgtc tccttctcga atgacgtccg gcctcggagc cgccagcacc
     3901 gcaactcgca tccgaagacg ctcgtccgga ccggagcgag cctcggcgcc gggacaatct
     3961 tgctgccggg ggtgaccgtg gggcggtggg cgatggtggg cgctgggtca ctcgtcaccc
     4021 gcgacgtgcc ggatttcgcg ctcgtctacg gcagcccggc gcgcgtgcac gggtccgtgt
     4081 gccgctgcgg cgagagcctc cggctggtcg atcgaagggc ggactgctcc tgcggccgcc
     4141 gctacgagct ccaaccggag gggggcgtgg cggagacgcc cgttccttga aggcaacggc
     4201 cgcgccgtcg tctgcgcgcg tccccttcgg atccctcgac gccgactacc gcgaacggcg
     4261 gggggcgatc gatgccgcga tcgcgcgcgt cctctcgcgc ggccgcttca tcctcgggga
     4321 agaagtcgaa tcattcgagc gggagctcgc ggcgtatctc gacgtccggc acgcggtggc
     4381 gtgcgcgagc gggacggacg cgatcgcgat cgcgctcgcc gcttcgggcg ccgggtcgga
     4441 agacgaggtc cttctcccgg ccaacgcgtg cgtgcccgtg atcgcggggg tccggctcgc
     4501 cggcgcgcaa ccgcggctgg ccgacgtcga gcgcgcaacg ctcacgctct cgcgggcggc
     4561 cgcggagcgg gcgctcggtc cgcgaacgag gtttctcctg gccgtccacc tctacggcgg
     4621 tctcgcggac gtcgagggcc tgcgggcgct cgcacgcgac cgtagtctcg tcctcgtcga
     4681 agactgcgcg cagagcctcg gggctctcgc ggacggaagg cgggccggct ccttcggatc
     4741 ggcatccgcg ttctccttct atccgaccaa gaatctcggc gcctacgggg acgccggctc
     4801 ggtcgccacg tcggacgatt ccatcgcggc gcgcgcgcgc ctcttgcgcc agtacggatg
     4861 gacccgtcgc gacttctcgg aagtggaagg gcgcaattcg cggatggacg agctgcaggc
     4921 ggcgatcctg cgcgccaagc tgccttacct cgaggcggac aacgcccggc gccgggagat
     4981 cgtccgccgc tacgacaagg gcctcgccga cctgccgctc cggcggccgg ccgccctcgc
     5041 ccggacgacg ccggctccgc acctctaccc ggtcctcgtg tcgtcgcgcg aggcgctccg
     5101 ggagcacctc gcaggacgcg ggatcgagac gggaatccac taccccgtcc ccatccacct
     5161 ccaaccggcg tacgccttcc tcggccacag ccggggcgac ttcccggtct cggaagaggc
     5221 gagcgagacg gtcctgtcgc tccctctcta tccgacgctc gccgacgacc aggtcgaggc
     5281 agtgatctcc gcgatgcgcg atttcttcgg tcgacctcga tgagcggcga gaggatctcc
     5341 gtcgtcgttc cggtctactt caacgcggag agtctgccgc gcctcgcgga tcggctccgt
     5401 gagctcgccg accgggccga cttcacgctc gagcccatct tcgtcgacga cggatccggc
     5461 gacgactcgt ggccgcgggt ccaggcgatc gtcgccgcct ggcccgtcgc gcgcgggatc
     5521 cgcctgaccc gcaacttcgg ctctcagatg gc
//
LOCUS       DASWAV010000432         2516 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U2665
            k141_580683, whole genome shotgun sequence.
ACCESSION   DASWAV010000432 DASWAV010000000
VERSION     DASWAV010000432.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960316
            Sequence Read Archive: SRR10260007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2516)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2516)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:47:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,929
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2516
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_580683"
                     /isolate="SMAG_U2665"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.36444 N 132.31889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..624)
                     /locus_tag="VF958_08995"
     CDS             complement(<1..624)
                     /locus_tag="VF958_08995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012646926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HEX9149281.1"
                     /translation="MRVFTGTSGFSYKEWKGSFYPEDLPDSGMLRFYASRLSAVEINN
                     TFYRMPRAPLLESWAEQVPDGFAFALKASQQITHRKRLKNAGEALAYFFGVATTLGDR
                     LGPALFQLPPNLKKDLPRLKDFLALLPPASRAAFEFRHESWFDAEVFDALRSARAALC
                     WAEDENLSTPPEPTADWGYLRLRRPDYGEAELAAHADRIRNQPWSDAF"
     gene            complement(697..972)
                     /locus_tag="VF958_09000"
     CDS             complement(697..972)
                     /locus_tag="VF958_09000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013010571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HEX9149282.1"
                     /translation="MKLYVGNLPYNISDDQLRDAFARYGTPDTATVIMDRSTGQSKGF
                     GFVEFHDAAIGKAALALDGTDFGGRSLKVNEARPRNESSGGYGGGRY"
     gene            1256..1933
                     /locus_tag="VF958_09005"
     CDS             1256..1933
                     /locus_tag="VF958_09005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020722069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HEX9149283.1"
                     /translation="MSDDGERPEDESKSSSGKVSRRDFLKIGGIAATVPLVGEKLVLA
                     AGAKVPVFGPGKVPVSLTVNGKVLRANLEPRVTLLDALRDHFDLTGAKRVCDRGTCGA
                     CTVLVDGKPVYSCSILAIEAQRRAITTVEGLGTPDNLHPVQRAFWDNDAQQCGFCTPG
                     FVVATKALLDRHPNPTAEQIENGLCGNLCRCGTYVGVRAAALQAAGARGAPAPSGALH
                     RGGRHDG"
     gene            1926..>2516
                     /locus_tag="VF958_09010"
     CDS             1926..>2516
                     /locus_tag="VF958_09010"
                     /inference="COORDINATES: protein motif:HMM:NF013482.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9149284.1"
                     /translation="MAEYPWPPAEKRKLIGKRISRVDGPEKLTGRAKYTYDIRRPGML
                     FGKIVRCPHAHARVKTIDTTAAEKLAGVRAIERMAEPGKEIFWAGDEVVALAAVDETT
                     AEDAARAIRIEYEVLPHFVSDAEPPADIPEGGPLEEREIRDMLGTMQVPAGQVVSRIE
                     REGVSFTPDQPKLDQLKQAGVPDAVLEALRRAKVSPP"
BASE COUNT          450 a          813 c          858 g          395 t
ORIGIN      
        1 gaaagcgtcg ctccacggct ggttgcggat ccggtccgca tgagcggcga gctccgcttc
       61 gccgtagtcg gggcggcgca gccgcaggta cccccagtca gccgtcggct ccggcggggt
      121 cgagaggttc tcgtcctcgg cccagcaaag cgcggcgcgg gccgaccgca gcgcatcgaa
      181 gacctcggcg tcgaaccagg actcgtgccg gaactcgaac gcggcgcggg aggccggcgg
      241 gagaagtgcg aggaagtcct tcagccgcgg caggtccttc ttcaggttcg gcggcagctg
      301 gaagagcgcg ggtccgagac ggtcgccaag cgtagttgcg acgccgaaga agtacgcaag
      361 ggcttcaccg gcgttcttga gccgctttcg gtgcgtgatc tgctgcgagg ctttgagcgc
      421 gaacgcgaac ccgtcgggga cctgctccgc ccagctctcg aggagcggcg cgcgcggcat
      481 tcggtagaag gtgttgttga tctcgacggc cgaaaggcgc gatgcgtaga agcggagcat
      541 cccggagtcg ggcaggtcct cggggtagaa gctccccttc cattccttgt acgagaaccc
      601 gctcgtgccg gtgaaaacgc gcatcgcgac attctatttt cgcgcgaaaa aaagccgccg
      661 gcgtcaccgc cggcggcaaa tcgaaaagac gacggtttag tagcggccgc cgccgtaacc
      721 gcccgaggac tcgttgcgcg ggcgcgcctc gttgaccttg agcgagcggc ctccgaaatc
      781 ggttccatcc agcgcgagcg ccgctttgcc gatcgccgcg tcgtggaatt cgacgaagcc
      841 gaaccccttc gactggccgg tgctgcgatc catgatcaca gtcgccgtgt cgggcgtgcc
      901 gtagcgcgcg aaagcgtcgc ggagctggtc gtccgagatg ttgtagggaa gattgccgac
      961 gtagagtttc atccgatcca tccttttgcc agggctgtcc tggccttcgc cgggagtaaa
     1021 gttcaatctc gaaacgccgc cggcgcgacg caccgaattc tcgtcattct gttcgttcgg
     1081 atttcgtcag agaagtgtct gaggagagcg agcgaggcga gaaccgttgc actctaccac
     1141 acttcacggc ctcgggacgg gatcgccggg aattgaatcc gtgtttcccg tcgggccggt
     1201 ttgtggcagc attgccccgg ctcgcttctc tttcacgaaa cggtggaggt gatcgatgag
     1261 cgacgacggc gaaaggccgg aagacgaatc gaaatcgagc tccggcaaag tctcccgccg
     1321 cgacttcctg aagatcggcg gcatcgccgc caccgttccg ctcgtcggcg agaagctcgt
     1381 tctcgccgcg ggagcaaaag tccccgtgtt cggccccggg aaggtcccgg tgtcgctgac
     1441 cgtcaacggc aaggtcctcc gcgcgaatct cgagccgcgc gtcaccctcc tcgacgcgtt
     1501 gagggaccac ttcgacctga ccggcgccaa gcgcgtctgc gaccgcggaa cctgcggagc
     1561 ctgcaccgtt ctcgtggacg ggaagccggt ctactcctgc tcgatactcg cgatcgaagc
     1621 tcagcgacgc gcgatcacga cggtcgaagg gctcggcact cccgacaacc tccacccggt
     1681 tcagagagcc ttctgggaca acgatgcaca gcagtgcgga ttctgcacgc cgggtttcgt
     1741 cgtggcgaca aaggccctcc tcgaccggca cccgaacccg acggccgaac agatcgagaa
     1801 tggcctctgc gggaacctct gccgctgcgg gacctatgtc ggggtgcggg ccgcggcgtt
     1861 gcaggccgcc ggtgcgcgtg gggcgccggc cccaagcgga gcgcttcacc ggggaggtcg
     1921 ccacgatggc tgagtacccg tggcctccgg cggaaaagag aaaactcatc ggaaaacgga
     1981 tctcgcgggt ggacggcccg gagaaactca cgggccgcgc gaagtacacc tacgacatcc
     2041 gacgcccggg gatgctgttc ggaaaaattg tccgctgccc gcacgctcac gcgcgcgtga
     2101 agaccatcga cacgaccgcg gcggagaagc tcgcgggagt gcgcgcgatc gagaggatgg
     2161 ccgagccggg caaggagatc ttctgggccg gtgacgaggt cgtggcgctc gccgccgtcg
     2221 acgagaccac ggcggaagac gccgcgcggg cgatccggat cgaatacgaa gtgctccccc
     2281 atttcgtgag cgacgccgag cccccggcgg acatccccga aggcggaccc ctcgaggaac
     2341 gcgagatccg agacatgctc ggcacgatgc aggtgccggc gggccaggtc gtctcccgca
     2401 tcgagcgcga gggcgtgagc ttcacccccg accagccgaa actcgaccag ctgaagcagg
     2461 cgggggtgcc ggacgccgtt ctcgaggcgc tgcggcgcgc gaaggtttcg ccgccg
//
LOCUS       DASWAV010000433         6179 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U2665
            k141_264004, whole genome shotgun sequence.
ACCESSION   DASWAV010000433 DASWAV010000000
VERSION     DASWAV010000433.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960316
            Sequence Read Archive: SRR10260007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 6179)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6179)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:47:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,929
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6179
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_264004"
                     /isolate="SMAG_U2665"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.36444 N 132.31889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..432)
                     /locus_tag="VF958_09015"
     CDS             complement(<1..432)
                     /locus_tag="VF958_09015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9149285.1"
                     /translation="MRTRKLETLSLAVLAGALVLGSAACQKRVETVVVVVTPTAGPVP
                     AAAYTPLPGLQPADTSPITADPIVVSQRNAVKRRTATQSRKASSASASATREGEPAEP
                     LRAAKEIPQGTILRVAFDEAISSAMAKVGDTLRGKLLDDVLA"
     gene            complement(514..2742)
                     /gene="pnp"
                     /locus_tag="VF958_09020"
     CDS             complement(514..2742)
                     /gene="pnp"
                     /locus_tag="VF958_09020"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011698097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HEX9149286.1"
                     /translation="MIHKTSIPVGDRSLDVEIGKVARQADGSAVVRFGDTVVLATAVF
                     AKEPKENADFFPLTVDYRENTYAAGRIPGGWFKREGRPTEKEILTSRLIDRPLRPLFP
                     KGFNHETQVIAFVLSADGQNDPDVLALNAASIALTCSLVPFYSPIGAVRVGSIAGQLV
                     LNPTNADRGRSEIDLIVAGTEEAVVMVEGGAREVSERRMLDAILFGHEAIRRIVASQK
                     ELQSKGGFQKPAWLAPVAYSPELFTQVRRQWEAPMRAALTMSGKINSYAEIKGVKKAA
                     VAVIPEDEADRRQQTARAVDDLVKVLTREVILNERKRLDGRAFDEVRPISCEVGLLPR
                     THGSALFTRGETQALVTATLGTSDDAQIIEEYEGESEHTFLLHYNFPPFSVGETKFMR
                     GPSRRDIGHGNLARRALSTVLPSEESFPYTVRVVSDILESNGSSSMATVCGGTLALMD
                     AGVPIKAPIAGVAMGLVGEGDRFAILTDIAGQEDHYGDMDFKVAGSAEGITALQMDIK
                     MKGLPRAVLEQALEQARAGRLHILQNMSSALERPRPNISPYAPRIFTMQIPRDRIRDV
                     IGSGGKTIRSIIEETGCKIDVEDSGKVAIASSDEAAALRAIQIIEGLTQEPEIGKIYT
                     GKVRRVEPYGAFVEILPGQDGLVHISELAPYRVRQTTDVVKEGDEVTVKIIAVDPTGK
                     IKLSRKQALSKEELETEMAMAPVGPGEEVGAREAERPREGDHFGHPNRDPRDRGPRGH
                     RK"
     gene            complement(2857..3132)
                     /gene="rpsO"
                     /locus_tag="VF958_09025"
     CDS             complement(2857..3132)
                     /gene="rpsO"
                     /locus_tag="VF958_09025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004511475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HEX9149287.1"
                     /translation="MNVTEHRAEKPNIVRQYRTHDNDTGSPEVQVALLTNRIVYLTDH
                     FKTNAKDHHSRRGLLKLVGRRRRLLDYLKAEDPERYRRLIERLGIRK"
     gene            complement(3244..4173)
                     /gene="truB"
                     /locus_tag="VF958_09030"
     CDS             complement(3244..4173)
                     /gene="truB"
                     /locus_tag="VF958_09030"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: protein motif:HMM:TIGR00431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004730 - pseudouridylate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HEX9149288.1"
                     /translation="MRPADPFRRGVLFLLDKPAGLTSHDVVERVRNAVALPRIGHSGT
                     LDPMATGLLLLCAGEAARLQGFFTLMDKSYDGVIRLGRATTTYDREGEAVGPERDAGG
                     IGRGEINAAADAFRGEFLQSPPPYSAKKVGGRKFYEMARKGESVPLVPKKVRVSELRF
                     GELADGRLPFTISCSSGTYIRSIASELGVKLGCGAHLESLRRTRIGQFHVSEAVPLER
                     FEGMSPAERLSAPHAVSLSRVQFPFERVRLASLETWRIRKGQSIPARGVSSREGDWVA
                     LVGPSDEMIALGQVTPMGTRGIALIRPKLVLAE"
     gene            complement(4170..4535)
                     /gene="rbfA"
                     /locus_tag="VF958_09035"
     CDS             complement(4170..4535)
                     /gene="rbfA"
                     /locus_tag="VF958_09035"
                     /inference="COORDINATES: protein motif:HMM:TIGR00082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HEX9149289.1"
                     /translation="MKKESRRSQRVADLVRSEISLLLLTEAHDPDVKTVTITEVEMPP
                     DLKSARVYFTARGDEAALQRAGEGLARAAGYLRREVGRRCGLRYAPELFFQPDRSYER
                     GARIDELLSEVLPEREEEP"
     gene            complement(4545..4832)
                     /locus_tag="VF958_09040"
     CDS             complement(4545..4832)
                     /locus_tag="VF958_09040"
                     /inference="COORDINATES: protein motif:HMM:NF016351.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF503 domain-containing protein"
                     /protein_id="HEX9149290.1"
                     /translation="MVLAYALFDLHLPGCRSLKEKRMIVRSLKARLRNEFEVSAAEVG
                     SQDLLERAELAVAAVGPDQVPLDQLLQNILTFVEENLDGELLNYQNEFIHV"
     gene            complement(4902..>6179)
                     /gene="infB"
                     /locus_tag="VF958_09045"
     CDS             complement(4902..>6179)
                     /gene="infB"
                     /locus_tag="VF958_09045"
                     /inference="COORDINATES: protein motif:HMM:TIGR00487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HEX9149291.1"
                     /translation="LVVAADDGVMPQTREAIAHARAAKVPIVVAINKIDKPNANPDRV
                     RKELADEGVLLESWGGDVPSAEISALKKQGIDQLLELLLIVADLQELSAAVGEQARGV
                     VLEARREAGRGNVATVLVQSGTLRVGDVFFAGSVFGRVRSMTDDRGARLSEAGPATPV
                     EVTGFEDLPQAGDAFQVVEDETKARSVASFRQLREREKAMAASQKLSLDQLFNKIQEG
                     RIKDLPLIVKTDVAGSAEVLSQALKNLSNEKVKVSLLHVGVGAININDVLLASASGAI
                     IVGFNVRPEKKAESEADKMGVEIRLYTVIYNVTDEIRKAMEGLLEPTLKEVARGRADV
                     RNTFRVPKFGVVAGCYITEGSVGRNSQVRLLRDSRVIYDGKIASLRRFKDDVSEVKQG
                     FECGIALERYQDVKIGDVIEAYQVEKVAGLMEA"
BASE COUNT          967 a         2039 c         2066 g         1107 t
ORIGIN      
        1 ggcgaggacg tcgtcgagga gcttcccgcg cagggtgtcg ccgaccttcg ccatcgccga
       61 cgagatcgcc tcgtcaaagg ccacgcggag gatcgtcccc tgcgggattt ctttcgcggc
      121 gcggagtggc tcggcgggct cgccctcgcg cgtcgcgctc gccgacgccg aggaggcctt
      181 ccgcgactgg gtggcagtgc gccgtttcac ggcgttcctc tgtgaaacga cgattggatc
      241 tgcagtgatc gggctcgtgt cggccggctg gagtcccggg agcggcgtgt aggcggcggc
      301 cggcacgggc cccgccgtcg gggtgacgac gacgacgacc gtctccactc gtttctggca
      361 cgcggcggac ccgagcacca gggctccggc caggaccgcc agcgacaatg tttcgagttt
      421 ccgggttcgc atctcagggc acctccttgt catcggccgt tggttgagca ggaaccgcgc
      481 cccggatagg cgcctggcgc agccgcacaa gactcacttg cgatgcccgc ggggaccccg
      541 gtcgcggggg tcccgattcg ggtgcccgaa gtggtcaccc tcccgcggac gctcggcctc
      601 tctcgccccg acctcctctc ccggtcccac gggcgccatc gccatctccg tctcgagctc
      661 ctctttcgag agagcctgtt tgcgggagag cttgatcttg ccggtggggt cgacggcgat
      721 gatcttgacg gtgacctcgt cgccctcctt gaccacgtcg gtcgtctggc ggacacggta
      781 cggagcgagc tccgagatgt ggacgaggcc gtcctgtccc ggcaggatct cgacgaaggc
      841 gccgtacggc tcgacccgtc ggaccttgcc ggtatagatc ttcccgatct ccggctcctg
      901 agtcagcccc tcgatgatct ggatggcgcg cagcgctgcc gcctcgtctg aggaagcgat
      961 cgccaccttg ccggaatcct cgacgtcgat cttgcagccg gtctcctcga tgatcgaccg
     1021 gatcgtcttg ccgcccgaac cgatcacgtc gcggatgcgg tcccgcggga tctgcatggt
     1081 gaagatgcgc ggtgcgtacg gggagatgtt cgggcgcggc ctctcgagcg ccgaagacat
     1141 gttctggaga atgtgcagtc gcccggcgcg cgcctgctcg agggcctgtt cgagaacagc
     1201 gcggggcagc cccttcatct tgatgtccat ctggagggcc gtgatgccct cggccgagcc
     1261 ggcgaccttg aagtccatgt cgccgtagtg gtcttcctgg ccggcgatgt cggtcaggat
     1321 cgcgaagcga tccccctcac cgacgagccc catcgccacg ccggcgatcg gcgccttgat
     1381 cggaacgccc gcatccatga gggcgagagt tcctccgcag accgtggcca tcgacgacga
     1441 gccgttggat tcgagaatgt cggaaacgac ccggacggtg tagggaaagg actcttcgct
     1501 cggcaggacc gtggacagcg cccgccgcgc gagattcccg tggccgatgt cgcggcggga
     1561 gggaccgcgc atgaacttcg tctcgccgac cgagaacggc gggaagttgt agtggagcag
     1621 gaacgtgtgc tcgctctccc cctcgtactc ctcgatgatc tgtgcgtcgt cggacgtccc
     1681 gagggtcgcc gttacgagcg cctgcgtctc gccgcgggtg aagagcgcgg aaccgtgagt
     1741 gcgcggcagg aggccgacct cgcaggagat cgggcgcact tcgtcgaagg cgcggccgtc
     1801 cagccgcttt ctctcgttca ggatgacctc acgcgtcagg accttcacga ggtcgtcgac
     1861 cgctcgcgcg gtttgctgac gccggtcggc ctcgtcctcg gggatcaccg ccaccgcggc
     1921 cttcttaacc cccttgatct cggcgtacga gttgatcttt cccgacatgg tcagggcggc
     1981 gcgcatcggc gcctcccact gccggcggac ttgcgtgaag agctccggcg agtacgccac
     2041 cggtgcgagc caggccggct tctggaagcc gcctttcgac tggagctcct tctgcgacgc
     2101 gacgatccgg cggatcgctt cgtgcccgaa gaggatcgcg tccaacatcc gccgttccga
     2161 tacctcgcgc gcgcccccct cgaccatcac gacggcctct tcggtgcccg caacgatgag
     2221 atcgatctcc gaacggccgc ggtccgcatt cgtgggattc aagacgagct gcccggcgat
     2281 cgagcccacg cggacggcgc cgatcggaga gtagaacggg acgagtgagc aggtcagcgc
     2341 gatcgacgcc gcattcagcg cgaggacgtc cggatcgttc tggccgtcgg ccgagagaac
     2401 gaacgcgatc acctgggttt cgtgattgaa gcccttcggg aagaggggcc gcagcggccg
     2461 gtcgatcagg cgggaagtca gaatctcctt ctcggtcggt cgtccctcac gcttgaacca
     2521 gccgccgggg atccggccgg cggcgtaggt gttctcccgg taatccacgg tgagggggaa
     2581 gaaatcggcg ttctccttgg gctccttggc gaagacggcg gttgcgagga ccacggtgtc
     2641 gccgaagcgc acgacggcgg agccgtcggc ctgtctcgcc acttttccga tctcgacgtc
     2701 gagggaccgg tctccaacgg gaatcgaggt cttgtgaatc atgatcgttc cttctggtct
     2761 tgtggccacc ggcggcgccg agtcgagcgg aaagaaagct gcccgggctg ccgcggggag
     2821 ggtctccggg cgggcccggc gccggccggg gcgcgtttac ttacggatgc cgaggcgctc
     2881 gatcaggcga cggtagcgct cggggtcttc ggctttcagg tagtcgagga gccggcggcg
     2941 gcggccgacg agcttcaaga gcccgcggcg ggagtggtgg tccttcgcgt tcgtcttgaa
     3001 gtggtccgtc aggtacacga tgcgattggt cagcagcgcg acctgcacct cgggagagcc
     3061 cgtgtcgttg tcgtgggtcc gatattgccg gacgatgttc ggcttctcgg ctcgatgctc
     3121 ggtgacgttc acttatcctc caaactcact ttctcactac ttttttctca atcggacagc
     3181 ctaccacacc ggagttgtcg cggggacagg tcctgcggac ctgcgcccgc cgcatttcaa
     3241 ccactattcg gccaggacga gtttcggccg gatgagcgcg atcccgcgcg ttcccatcgg
     3301 cgtcacctga ccgagagcga tcatttcgtc ggacggcccg acgagagcca cccagtcccc
     3361 ctcgcgtgaa gagacgcccc gcgccggaat cgactgtccc ttgcggatcc tccacgtctc
     3421 cagggaagcg aggcgcaccc gctcgaaggg aaactgcacg cgcgagagcg agacggcgtg
     3481 cggcgccgaa agccgctcgg cgggcgacat gccttcgaat cgctcgagcg ggaccgcttc
     3541 ggaaacgtgg aactgtccga tgcgagtccg gcgcagggac tccaggtggg cgccgcagcc
     3601 gagcttcacg ccgagctcgc tcgcgatcga gcgaatgtag gtgcccgagg agcacgagat
     3661 cgtaaaggga agacgaccgt cggcgagttc gccgaaacgg agttcgctga cgcggacttt
     3721 cttcggaacg agcggaacgc tttcgccctt gcgcgccatc tcgtagaact tgcgtccgcc
     3781 gactttcttt gcggaataag ggggcggcga ctggaggaac tctccccgga atgcatcggc
     3841 ggccgcgttg atctcgccgc gcccgattcc gccggcgtcg cgctcgggac cgacggcttc
     3901 gccctctcga tcgtacgtcg tcgtggcgcg gccgagccgg atgacgccgt cgtacgactt
     3961 gtccatcagc gtgaagaacc cctggagtcg cgccgcttct cccgcgcaga ggaggaggag
     4021 gcccgtggcc atcgggtcga gcgttccgct gtgtccgatg cgcggcaggg ctacggcgtt
     4081 gcgcacgcgc tccacgacgt cgtgggacgt gagcccggcc ggtttgtcga ggagaaagag
     4141 gacgccccgg cggaagggat cagcgggtct cacggctcct cttcgcgctc cggcaggact
     4201 tccgagagaa gctcgtcgat ccgcgcgccg cgctcgtagg agcggtccgg ctgaaaaaag
     4261 agctccggcg cgtaccgcag tccgcagcgc cggccgacct cgcggcgcag gtaaccggcg
     4321 gctcgcgcga gaccctctcc ggcgcgctgg agggcagcct cgtcgccgcg tgccgtgaag
     4381 tagacgcgcg cggacttcag gtctggcggc atctcgacct cggtgatcgt cacagtcttc
     4441 acgtccggat cgtgcgcctc ggtcaggagg agcagagaaa tctcggacct cacgaggtcg
     4501 gcgacgcgct ggctccggcg ggattccttc ttcatcgcgg cacgtcagac atggatgaat
     4561 tcattctggt aattgaggag ctctccgtcg aggttctcct ccacgaacgt cagtatgttc
     4621 tggaggagct ggtcgagcgg gacctggtcg gggcctaccg ccgcgacggc gagctccgcg
     4681 cgttcgagca ggtcctggga gcccacttcc gccgcggaaa cctcgaactc gtttcggagc
     4741 cgcgccttca agctccggac gatcattcgc ttctccttca ggctccgaca ccccggcagg
     4801 tgaaggtcga acagggcgta ggcgaggacc atcagaatcg gaagtcggaa atcgaagccg
     4861 gagatcggtg ctctcgatga ccgattcccg actcccgcgc ctcaggcctc catcagcccg
     4921 gcgaccttct cgacctggta ggcctcgatg acgtcgccga tcttcacatc ctggtatctc
     4981 tcgagcgcga tgccgcactc gaatccctgt ttcacttcgg agacgtcgtc cttgaaccgg
     5041 cggagcgagg cgatcttgcc gtcgtagatg acgcggctgt cgcgcaggag gcggacctgc
     5101 gagttgcgtc cgaccgagcc ctcggtgatg taacagcccg ccacgacgcc gaacttcggc
     5161 acgcggaacg tgttgcgcac gtccgcacgc ccgcgcgcga cctctttcaa cgtcggttcg
     5221 agcaggccct ccatcgcctt ccggatctcg tcggtgacgt tgtagatgac ggtgtagagg
     5281 cggatctcga cgcccatctt gtcggcttcc gactccgcct tcttctccgg gcgcacgttg
     5341 aacccgacga tgatcgcacc ggaggccgag gcgagcagga cgtcgttgat gttgattgcg
     5401 ccgacaccca cgtgaaggag cgagaccttc accttttcgt tcgacaggtt cttcagggcc
     5461 tgggaaagaa cttcggcgga tccggccacg tctgtcttca cgatcagcgg gagatccttg
     5521 attcggccct cctggatctt gttgaagagc tgatcgagcg acagtttctg gcttgccgcc
     5581 atcgcctttt cccgctcacg cagctgacgg aaagaggcga ccgagcgcgc cttggtctcg
     5641 tcctcgacga cctggaacgc gtcgccggcc tgcggaaggt cctcgaagcc cgtgacttcg
     5701 accggggtcg ccgggccggc ttcggagagc cgggcgccgc gatcgtcggt catggaacgg
     5761 actcttccga agacggagcc cgcgaagaag acgtcgccga cgcggagcgt gccggactga
     5821 acgagcacgg tagccacgtt gccgcggccc gcctcgcggc gtgcctcgag cacgactccg
     5881 cgcgcctgtt ctccgactgc agcggagagc tcctgaagat cggcgacgat caacagcagc
     5941 tcgagcagct ggtcgatccc ctgcttcttg agcgccgaaa tctcggcgga cgggacgtcg
     6001 cctccccacg actcgaggag gacgccttcg tcggcgagct ccttgcgcac gcggtcggga
     6061 ttcgcattcg gcttgtcaat cttgttgatc gcgacgacga tcggcacctt cgcggcgcgg
     6121 gcgtgggcga tcgcctctct ggtctgcggc atcacgccgt cgtccgcggc aacgacgag
//
LOCUS       DASWAV010000434         2988 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U2665
            k141_744478, whole genome shotgun sequence.
ACCESSION   DASWAV010000434 DASWAV010000000
VERSION     DASWAV010000434.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960316
            Sequence Read Archive: SRR10260007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2988)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2988)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:47:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,929
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2988
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_744478"
                     /isolate="SMAG_U2665"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.36444 N 132.31889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1450
                     /locus_tag="VF958_09050"
     CDS             <1..1450
                     /locus_tag="VF958_09050"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX9149292.1"
                     /translation="TGLVEVAGGLLTAALALGILFYLSVKLTLITVGVLSLFGVVLFY
                     AFKTLRPLFRERSKINAEVSGRLMESFSGIRVVKAYRAERREALVFSKGVHRLFRNVV
                     KTMTGFAAVSAFSTLLLGIVGVAIMWIGSHEVLAHRMTLGSFFSFTLYLGMLVGPVIQ
                     IVSIGSQITEAFAGLERIREIRDEKREGEGDAALEPLPRIVGDVEFDDVSFEYEKGVA
                     VLRNVSFEARAGTSTALVGPSGAGKSTLIGLVAAFYRPTSGVIRVDGRDLSHVRLADY
                     REQLGVVFQDNFLFDGTVLENIAYARPEASRDEVLRAARIARCDDFIRELSDGYETVV
                     GERGVKLSGGQRQRVAIARAVLADPRILILDEATSSLDSESEAAIQEGLAELMKGRTT
                     FVIAHRLSTIRHADTILVLEGGQIVERGNHRDLLARGGRYFDLYTRQYGLEANLFLNP
                     GETPAVDEQEAAPQSSADASVVRLPLVRSPGE"
     gene            1468..1947
                     /locus_tag="VF958_09055"
     CDS             1468..1947
                     /locus_tag="VF958_09055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012962904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX9149293.1"
                     /translation="MAQRRDEAIGFWEEGTSKLLTGDVDGAIRLFTRSLELEPTAEAY
                     TFRGWAYSFQGRIDDAIEECRKAIATDPAFGNPYNDIGCYLMQKGELEEAVPWFEQAK
                     KAPRYEPRHYPYLNLGRLHAARGDLARAVEEFEGALAESPEDPIAMKFLEALKYKVN"
     gene            2048..2752
                     /locus_tag="VF958_09060"
     CDS             2048..2752
                     /locus_tag="VF958_09060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018097388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HEX9149294.1"
                     /translation="MTPESAFQAGALTVSADRVTAFLRKVYGWMFVGLAITAAVAFAV
                     ASSPTLVRAIFGNPILFIVLMVAELGLVVALSARVDRMAPTTAAVLFVTYSALTGATL
                     SAILLAFTGASLATTFVVAAGMFGALALYGTTTKRSLAGMGQFLFMGLIGVVLASVVG
                     LFWHAPGFQFGISVVGVLVFTGLTAWDSQRLKEMAAVTPDGQVAGRAVVGALRLYLDF
                     INLFLFLLRFLGARRD"
     gene            2760..>2988
                     /locus_tag="VF958_09065"
     CDS             2760..>2988
                     /locus_tag="VF958_09065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9149295.1"
                     /translation="MTPRRAVSGLLILAGAGLIGAAALHYSRGSRAQEEGRETLARSM
                     EGGYAAVSAPAVSARIEGASAATDDLPRAGAE"
BASE COUNT          444 a         1014 c          984 g          546 t
ORIGIN      
        1 caccggcctg gtcgaagtcg ccggcgggct tctgaccgca gcgctcgctc tcgggattct
       61 cttctacctc tcggtgaagc tgacgctgat cacggttggc gtcctctccc tcttcggcgt
      121 cgtgctcttc tatgccttca agaccctccg cccattgttc cgggaacgtt cgaagatcaa
      181 cgcggaggtc tccggccgcc tgatggagtc gttctccggc attcgggtgg tcaaggcgta
      241 ccgcgccgag cggcgtgagg ccctcgtctt ttcgaaaggg gtccaccggc tcttccggaa
      301 cgtcgtgaag acgatgacgg gcttcgcggc ggtttccgcg ttctcgactc ttctcctcgg
      361 gatcgtcggc gtcgcgatca tgtggattgg gtcgcacgaa gtcctcgcgc accggatgac
      421 gctcggttct ttcttctcgt tcacccttta cctggggatg ctcgtggggc cggtcatcca
      481 gatcgtttcg atcgggagcc agatcaccga ggctttcgcc ggcctcgagc gcatacgcga
      541 gatccgggac gagaagcgcg agggcgaggg cgacgccgcg ctcgaaccgc tcccgcgcat
      601 cgtgggcgac gtcgagttcg acgacgtttc cttcgagtac gagaagggcg tggcggtcct
      661 tcggaacgta tcgttcgagg cgcgggcggg cacgtccacg gcgctcgtcg gcccttccgg
      721 cgccggcaag agcacgctca tcgggctcgt cgccgcgttc taccgcccga cgtccggcgt
      781 catccgggtc gacggtcgcg acctctccca cgtgcgcctc gcggactacc gtgagcagct
      841 cggcgtcgtc ttccaggaca actttctctt cgacggtacg gtgctcgaga acatcgcgta
      901 cgcgcgcccc gaggcgtcgc gggacgaagt ccttcgcgcc gcccgcatcg cccgctgcga
      961 cgacttcatc cgcgaacttt cggacggcta cgaaacggtg gtcggagagc gtggcgtgaa
     1021 gctctccggc ggtcagcgcc agcgtgtcgc gatcgcgcgc gcggtcctcg ccgacccgcg
     1081 catcctgatt ctcgacgagg cgacttcgtc gctcgacagc gagagcgagg cggccatcca
     1141 ggaaggcctg gccgagctca tgaagggccg gacgaccttc gtcatcgcgc accgcctctc
     1201 gacgatccgc cacgcagaca cgatcctcgt gctcgaaggg ggccagatcg tggaacgcgg
     1261 aaaccatcgc gacctgctcg cacgcggagg ccgctacttc gatctctata cgcgtcagta
     1321 cggcctcgag gcgaacctct tcctgaaccc gggggagacg ccggcggtcg acgaacagga
     1381 agcggcaccg cagagctcgg ccgacgcgag cgttgttcga cttcctctcg tgagatcgcc
     1441 gggagagtga cggcttagaa tcgttccgtg gcgcagagaa gagacgaagc gatcgggttc
     1501 tgggaagagg gcacctcgaa gctgctgacg ggcgacgtcg acggcgccat ccgcctcttc
     1561 acccggtccc tcgagctcga gccgacggcc gaggcctaca cgttccgcgg gtgggcgtac
     1621 tcgttccagg gccgcatcga cgacgcgatc gaggagtgcc ggaaggcgat cgccaccgac
     1681 ccggccttcg gcaatcctta caacgacatc ggctgctatc tgatgcagaa gggcgagctc
     1741 gaggaagccg tcccgtggtt cgaacaggcg aagaaggctc cacgatatga gccgcgccac
     1801 tatccgtatc tgaacctcgg gcggctgcac gccgcgcgcg gagatctcgc acgggcggtc
     1861 gaggagttcg agggcgccct cgcggagagc cccgaggacc cgatcgcgat gaaattcctg
     1921 gaggccctca agtacaaggt caactgaggt ggattccggc acagtcgcgg aatgcgccgc
     1981 aggccccgtc cgttttcatt gagcggtttc gaaagagagg agagatctcg acatggctcg
     2041 tttcgaaatg actcccgaga gcgcctttca ggcgggcgcg ctgaccgtca gcgccgaccg
     2101 cgtcacggca tttctccgca aggtctacgg ctggatgttc gtcgggctcg cgatcaccgc
     2161 agccgtcgca ttcgccgtgg cgagctcgcc gacgctcgtc cgggcgatct tcggcaaccc
     2221 gatccttttc atcgttctga tggtcgccga gctcgggctc gtcgtcgcgc tctccgcccg
     2281 tgtggaccgc atggccccca ccacggcggc ggtcctgttc gtgacctact cggcgttgac
     2341 cggcgccact ctctcggcca tcctcctggc gttcacgggc gcgtcgcttg caaccacgtt
     2401 cgtcgtggcc gccgggatgt tcggagcgct cgcgctctac ggaacgacga cgaagagaag
     2461 cctcgccggc atggggcagt tcctcttcat gggtttgatc ggcgtcgttc tcgcgtccgt
     2521 cgtcggtctc ttctggcacg ccccgggttt ccagttcgga atttcagtcg tgggcgttct
     2581 cgtcttcacc gggttgaccg cgtgggattc gcagagactg aaggaaatgg cggcggtcac
     2641 gccggacggc caggtcgccg gccgcgcggt cgtgggagcg ctccggctct acctcgactt
     2701 catcaacctc ttcctgttcc tcctgcgttt cctcggcgcg cgtcgggact gagattcccg
     2761 tgaccccgcg ccgcgccgtc tccgggctcc tgatcctcgc cggcgcggga ctgatcggcg
     2821 ccgcggcgct gcactactcg cgcggctcgc gcgcccagga ggaggggcgg gagacgctcg
     2881 cccgcagcat ggagggcggc tacgcggcgg tttcagcgcc ggccgtctcc gcgcggatcg
     2941 aaggcgcgtc cgccgccacc gacgacctcc cccgggccgg cgccgagc
//
LOCUS       DASWAV010000435         3270 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U2665
            k141_425281, whole genome shotgun sequence.
ACCESSION   DASWAV010000435 DASWAV010000000
VERSION     DASWAV010000435.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960316
            Sequence Read Archive: SRR10260007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 3270)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3270)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:47:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,929
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3270
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_425281"
                     /isolate="SMAG_U2665"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.36444 N 132.31889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2037
                     /locus_tag="VF958_09070"
     CDS             <1..2037
                     /locus_tag="VF958_09070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002624802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosidase"
                     /protein_id="HEX9149296.1"
                     /translation="EGYWPRPRISREAGGALLAEHAGLGRFRLEAEGKPVEPTVLFTE
                     NETNFSRLFGSPNPQPWVKDAFHEYVVHSRDAVNPEEIGTKAALHYALDVPARGEVTL
                     RLRLSSEEEAPGDAFADHDQVFAVRRRETKSFYEALIPPGITDEEVIVGRQAYAGLLW
                     TKQFYHFAVRDWLEGDPSQPPPPVARKRGRDSAWTHLYNRDVISMPDKWEYPWYAAWD
                     LAFHMIPFARLDPDFAKEQLVLLLREWYMHPNGQIPAYEFAFGDVNPPVHAWAAWRTY
                     KMTGPRGARDRFFLARVFQKLLLNFTWWVNRKDPEGKNLFAGGFLGLDNIGVFDRSRE
                     LPTGGHLAQADGTAWMAFYCLTMLSMALELASEDPAYEDVASKFFEHFVAIADSMNTL
                     GGTGLWDEEDGFYYDQLHVDGHQLPLKLRSMVGLVPLFAVEVLEESVIAKLPGFRKRM
                     QWFLENRRDLARHIAYMEGREGDAHGHRLLAIPTKDRLERVLRYLFDESEFLSPHGIR
                     ALSRVYGERPYTFSVAGHEYRVGYEPGESTTGVFGGNSNWRGPVWFPVNYLLIECLER
                     YHHYYRSELTVEFPTGSGRRMNLGEVACELARRLSSLFLPDESGRRPCHGDDLRYARD
                     PNWKELVLFHEYFHGETGRGVGASHQTGWTALVLRLLRDVATSRSLARETDSPR"
     gene            2098..2883
                     /locus_tag="VF958_09075"
     CDS             2098..2883
                     /locus_tag="VF958_09075"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEX9149297.1"
                     /translation="MTPRHGAGAGAFRMPNDWWKDFFSGLIVDFWQAVMPEETTKAEA
                     DFLEKHLQLSSPGTRVLDVPCGGGRLAIELAARGCRLVAVDISPQFLEEGREAARERG
                     LTVEWRQADMRDLPWRGEFDAAYCAGSSFGFLGDDGDREFLSAVARALKPGGRFFADF
                     KAAESILLNFRERYEMRIGDIEFQARNVYDPITATMRSEYTVSRGDRSETKEAVHRIY
                     TAREILRMLEHAGFSTFETFGSIEGEPFRLGSATLVVVAEKNR"
     gene            2990..>3270
                     /locus_tag="VF958_09080"
     CDS             2990..>3270
                     /locus_tag="VF958_09080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9149298.1"
                     /translation="MARFGGIGGGLGPLPRGDRRGGRAMRSGSAEEKKRKAPPFKVVM
                     RDAAELVQQRKGRLAVGFGLLAVNRICGLVLPGTTKFLLDEVIGKGRREL"
BASE COUNT          551 a         1081 c         1091 g          547 t
ORIGIN      
        1 gagggctact ggccgaggcc gcgaatctcg cgcgaagccg gcggcgcgct cctcgccgag
       61 cacgccggcc tcgggcggtt ccggctggag gcggagggga agccggtcga gccgaccgtc
      121 ctgttcacgg aaaacgagac gaacttctcg cgtctcttcg gctcgccgaa ccctcagccc
      181 tgggtgaagg acgcgtttca cgagtacgtg gtccacagcc gtgacgcggt caacccggag
      241 gagatcggta cgaaagccgc gctgcactac gcactcgacg tgccggctcg aggtgaagtg
      301 acgctccggc tgcgcctctc ctcggaagag gaggcgcccg gagacgcctt tgcagaccac
      361 gaccaggttt tcgccgtccg gcggcgagag acgaagagct tctacgaggc gctcatccct
      421 cctggaatca ccgacgaaga ggtgatcgtc ggacgccagg cgtacgccgg tctgttgtgg
      481 accaagcagt tctaccattt cgccgtaagg gactggctcg aaggcgatcc gtcgcagccg
      541 ccgccgcccg tggcgcgaaa gcgtggccgt gacagcgcct ggacgcacct ctacaaccgc
      601 gacgtgatct cgatgccgga caagtgggag tacccgtggt acgcggcatg ggacctcgca
      661 ttccacatga ttccgttcgc gcgcctcgac cccgacttcg cgaaggagca gctcgtcctg
      721 ctcctccggg agtggtacat gcacccgaac ggccagatcc cggcttacga gttcgcgttc
      781 ggcgacgtga acccgccggt gcatgcgtgg gcggcgtggc gaacgtacaa gatgacgggg
      841 ccccggggcg cgcgcgaccg gttcttcctc gcacgcgtgt tccagaagct ccttctcaac
      901 ttcacgtggt gggtcaaccg caaggacccg gagggcaaga acctcttcgc gggcgggttc
      961 ctcgggctcg acaacatcgg cgtcttcgac cgatcgcgcg agctcccgac cggcggccat
     1021 ctcgcgcagg ccgacgggac cgcgtggatg gcgttctact gcctgacgat gctctcgatg
     1081 gccctcgagc tcgcgagcga agaccccgcg tacgaggacg tggcgtcgaa gttcttcgaa
     1141 cacttcgtcg cgatcgccga ctcaatgaac acgctcggcg gcactgggct gtgggacgag
     1201 gaggacgggt tctactacga ccagctccac gtcgacggcc accagctccc gctcaagctc
     1261 cgctcgatgg tcggcctcgt cccgctcttc gcggtggagg tgctcgagga gtccgtcatc
     1321 gcgaagctcc ccggctttcg caagcgcatg cagtggttcc tggagaaccg cagggacctc
     1381 gcgcgtcaca tcgcgtacat ggagggaaga gagggcgacg cccacggcca ccggctcctg
     1441 gcaatcccca cgaaagatcg gctcgaacgc gtcctccggt atctgttcga cgagagcgag
     1501 ttcctatcgc cgcacggaat ccgcgcgctc tcgcgcgtct acggcgagcg gccctacacc
     1561 ttctcggtgg ccggtcacga ataccgggtc ggctacgagc ccggcgaatc cacgacgggg
     1621 gtcttcgggg gcaactcgaa ctggcgaggc cccgtgtggt ttccggtgaa ctaccttctc
     1681 atcgagtgtc tcgagcgcta ccaccactac taccggagcg aactcaccgt cgagttcccg
     1741 acgggctccg ggcgccggat gaatctcggg gaggtcgcgt gcgagctcgc ccgcagactc
     1801 tcgtctctgt tccttccgga cgagagcggc cgccgcccct gccacggcga cgacctccgc
     1861 tacgcccggg atccgaactg gaaggagctc gtcctctttc acgagtactt ccacggcgag
     1921 accgggcgcg gcgtcggagc gtcccaccag accggctgga ccgctctcgt gctgaggctg
     1981 ttgagagacg tcgcaacgtc gcgctcgctt gctcgggaaa cggattctcc gcggtagact
     2041 cggctcctgc ctatgatcgt tcgctgaacc cctgccgctt tcccagactc gaaaaccatg
     2101 acgccgcgcc acggcgcggg agcaggagcg ttcagaatgc ccaacgactg gtggaaagac
     2161 tttttcagcg ggctcatcgt cgatttctgg caggcggtga tgccggaaga gacgacgaaa
     2221 gccgaggccg attttctcga gaagcatctc cagctctcct ctccgggcac ccgtgtcctg
     2281 gacgtgccgt gcggcggagg ccgcctggcg atcgagctcg cggcgcgcgg atgccggctg
     2341 gtggccgtcg acatttctcc gcaattcctc gaagagggac gggaagcggc acgagagaga
     2401 ggtctcaccg tcgagtggcg acaggcggac atgcgcgatc tgccctggcg cggcgagttc
     2461 gacgcggcgt attgcgccgg gtcgagcttc gggtttctcg gggacgacgg agaccgggag
     2521 ttcctctccg ccgtcgcccg cgccttgaaa ccgggcggcc gcttcttcgc ggacttcaag
     2581 gcggccgagt cgatcctgct gaactttcgg gagcgatacg agatgcgcat cggagacatc
     2641 gagttccagg cacgcaatgt ctatgacccg atcactgcaa cgatgcgaag cgagtacacg
     2701 gtctcgcgcg gcgatcgaag cgaaacgaag gaggccgtcc accggatcta caccgcgcgc
     2761 gagatcctgc ggatgctgga gcacgccggg ttttcgacgt tcgagacgtt cggctcgatc
     2821 gagggagagc cgttccggct cggctcggcg acgctggtgg tcgtcgcgga gaagaaccga
     2881 tagcctttct gcgccgggaa ggcgcgtatc cccaggctat ccctcgacct cgacttccgg
     2941 aggccggcgc acctgcgccg gcttttcttt cataggtaga ctcgaccgga tggcgagatt
     3001 cggagggatc ggcggcgggc tcggccctct cccccgcggc gaccgtcgcg gcggacgcgc
     3061 gatgcgctcc ggatcggcgg aggagaagaa gcgaaaggcg ccgccgttca aggtcgtcat
     3121 gcgcgacgcc gccgagctcg ttcagcagcg caagggtcgc ctcgcggtcg ggttcggcct
     3181 gctggccgtc aaccgcatct gcgggctggt cctccccggc accacgaagt tcctgctcga
     3241 cgaggtcatc gggaagggac ggcgcgagct
//
LOCUS       DASWAV010000436         4285 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U2665
            k141_704708, whole genome shotgun sequence.
ACCESSION   DASWAV010000436 DASWAV010000000
VERSION     DASWAV010000436.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960316
            Sequence Read Archive: SRR10260007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 4285)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4285)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:47:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,929
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4285
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_704708"
                     /isolate="SMAG_U2665"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.36444 N 132.31889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..255
                     /locus_tag="VF958_09085"
     CDS             <1..255
                     /locus_tag="VF958_09085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9149299.1"
                     /translation="GRELFFITQSRTIMATPFDSLSPDLPAGPARRLFDVMMHRQFSS
                     NVAYRYDVAPDGRFLIVVRFSEEVPALILVTNWQAGLEKK"
     gene            252..2933
                     /locus_tag="VF958_09090"
     CDS             252..2933
                     /locus_tag="VF958_09090"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HEX9149300.1"
                     /translation="MTLTSGTRLGPYEIVAAVGAGGMGEVYRAKDTRLDRTVAVKVLP
                     AHLSSSAESRQRFEREAMTISSLSHPHICALYDVGNQDGVEYLVMEYLEGETLSDRLV
                     KGPLAFDQVLRYGLEIADALDKAHRQGIVHRDLKPGNVMITKSGVKLLDFGLAKAVAP
                     QAARSGVSLTALPTQQVRAELTAEGSILGTLQYMSPEQLEGGEADGRTDIFALGAVLY
                     EMATGKKAFSGKSQASLISSIMGSEPAPVSAISPVTPPAFDRLVGTCLAKDPDDRWQT
                     AHDIAVQLRWIQEGGAAAGIAAPVVPRRKRRDRIAWALAALATAGLTAVLMRDMRRSP
                     LPAPVTRFVILAPEKTMFTPPGELSSAQMALSPDGRTLAFVANPAVARPLLWIRSLDS
                     FNPVPLAGTEDALHPFWSPDGRSIGFFTQTSLKRVEASGGTPQKLCDAIAGRGGSWSR
                     EGVIVFANSSPTPLFRVPDTGGEPQPATEFDAARAEDRHRFPFFLPDGRRFLFWSRSS
                     QPQHTGIYVASLDSRKGRLVVKSEAMGVYAAGYLLTAQQGMLVAYPIDEKTAQVRGNP
                     VRLAEGVVSANPPGYAPFSATDTTIAYSAAFAKSRQLVWFDRSGRRLSSVGEPADYST
                     PRLSPDEKRIAVAIREEGKPNTDIWIFDTIRTTWSRFTFDPAAERAPLWSPDASRIVF
                     TSASRGILDLYEKPASGSGEPHLVVSSLSDKFPTDWTRDGRFLIYHSFGSGSSTWDIW
                     AAPTDGGKPLALFASKFTEAHGRVSPDGRWIAYASDESGRFEIYVTQFPEKRGRWQIS
                     TTGGSQPWWRGDGKELFYLGPEQTLMSVAVRPGDAFEAAVPTSLFKANFPTLVPAFWP
                     NYCATADGQRFLITELVAEVVASPINVVLNWTAALKK"
     gene            complement(2956..>4285)
                     /locus_tag="VF958_09095"
     CDS             complement(2956..>4285)
                     /locus_tag="VF958_09095"
                     /inference="COORDINATES: protein motif:HMM:NF014081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX9149301.1"
                     /translation="GGTLVDGTGAAPVPNATVVLRGGKIDCAGRCAIPGGVTVVDARG
                     MWVTPGLVDAHVHFSQTGWADGRPDALDVRSTHPYEKVEADLKEHPDRFFRSQLCSGI
                     TTVFDVGGYPWTVAMAHRERQDTRAPRVGAAGPLLSTLDHWLNLPAERQFILLKDAES
                     ARNGVAYLKSVGAEAVKVWYIVRPELPVEKSAPVVLAAGEEARKAGLPLIVHATGLAE
                     AKVALRAGAGVLVHSVIDLPVDEEFLSLAKKNGTIYCPTLTVFGGYVRMAQGVSSKKP
                     PAVDDPNGCVDAETLAKVAETARVPTPEGYAVQVTRQEERAVRAEQIGKANLKRVVDA
                     GIPIAMGTDAGNPLTLHGPSVYAEMEAMQSAGMTPMQVIVASTREGAAAMGMEKETGT
                     VEKGKSADLLIVGGEPTADVANFRKVRYVVRGGVVRSIEELKATVAAGSK"
BASE COUNT          672 a         1495 c         1380 g          738 t
ORIGIN      
        1 ggccgggagc tcttcttcat cacacagagc cgcacgatca tggcgacgcc tttcgacagt
       61 ttgagccccg acctgccggc cggccccgcg cggcggctct tcgacgtcat gatgcaccgc
      121 cagttcagct cgaacgtggc ttaccggtac gacgtcgctc cggatggccg cttcctcatc
      181 gtcgttcgtt tctctgagga ggttcctgcg ctgattctcg tgacgaactg gcaggcgggt
      241 ctcgagaaga aatgactctg acttccggca cccgcctggg accgtacgag atcgtcgccg
      301 cggtcggcgc cggcgggatg ggggaggtct accgggcgaa ggacacgcgg ctcgaccgca
      361 cggtcgcggt aaaggtgctg ccggcgcatc tgtcctcatc ggccgagtcg cggcagcgct
      421 tcgagcgaga ggcgatgacg atctcgtctc tttcgcaccc gcacatctgc gcgctgtacg
      481 acgtcggcaa ccaggacggc gtcgagtacc tggtcatgga gtacctggag ggcgagacgc
      541 tgtcggacag gctcgtgaag gggccgctcg cgttcgatca ggtgctgcgt tacgggttgg
      601 agatcgccga cgctctcgac aaagcgcacc ggcaggggat cgtgcaccgg gatctgaagc
      661 cgggaaacgt gatgatcacg aagtcggggg tcaagctcct cgacttcggg ctcgccaagg
      721 cggtggcgcc gcaggcggcg cgctccgggg tctcgctgac cgcccttccg acgcagcagg
      781 tccgagccga gctgaccgcc gagggatcga tcctgggcac gctccagtac atgtcgcccg
      841 agcagctcga gggcggggag gccgacgggc gcaccgacat cttcgcgctc ggcgcggtgc
      901 tctacgagat ggcgacgggg aagaaggcgt tctccggcaa gagccaggcg tccttgattt
      961 cctcgatcat gggatcggag cctgcgccgg tctcagcgat ctcgccggtg acgccgcccg
     1021 cgttcgatcg gctggtcggc acgtgtctcg cgaaagaccc ggacgatcgg tggcagaccg
     1081 cgcacgacat cgccgtgcag ctcaggtgga tccaagaggg cggggccgcg gccggaattg
     1141 cggcgccggt cgtgccccgg aggaagagac gcgaccgcat cgcgtgggct ctcgcggctc
     1201 tcgccaccgc gggcttgacc gccgtcctca tgcgcgatat gcggcgatca cccctccccg
     1261 ccccggtcac gcgattcgtg attctcgcgc ccgagaaaac gatgttcacg ccccccgggg
     1321 agctctcctc ggctcagatg gctctttcgc ccgacgggcg aacgctcgct ttcgtggcca
     1381 atcccgcggt ggcgcgacct ctcctgtgga tccgttccct cgacagtttc aatcccgtcc
     1441 cgcttgctgg caccgaagac gctcttcatc ccttctggtc tcccgacggg cgatccatcg
     1501 ggtttttcac acagacgagc ctgaaaagag tcgaggcatc gggcgggacc ccgcagaagc
     1561 tttgcgacgc aatcgcaggc cgaggcgggt cgtggagccg agaaggcgtg atcgtgttcg
     1621 cgaacagctc gccgacgccg ctctttcggg tgccggacac cggcggagaa ccgcagccgg
     1681 cgaccgaatt cgacgccgcg cgcgcggagg accggcatcg attcccgttc tttctgccgg
     1741 atggaaggag gttcctgttc tggtcgcgaa gcagccaacc gcaacacacc ggtatttatg
     1801 tggcgtccct cgactcccgg aagggccggc tggtcgtcaa gtcggaggcg atgggtgtct
     1861 acgcggcggg atatctgctc accgcccagc agggaatgct cgtcgcctac ccaatcgacg
     1921 aaaaaaccgc gcaggtgcgc ggtaatcctg tccgcctggc ggaaggcgtc gtcagcgcga
     1981 acccccccgg gtacgcgccg ttctccgcca ccgacacgac gattgcctac tccgcggcat
     2041 tcgcgaaaag ccgacagctg gtctggttcg accgttccgg acggcgtctc agcagcgtcg
     2101 gcgaacccgc cgactacagc accccgcgcc tttccccgga cgagaagagg atcgcggtgg
     2161 cgatccggga agagggaaag ccgaacaccg acatctggat cttcgacacc atccgaacga
     2221 cgtggtcgcg ttttaccttc gatcccgccg ctgagcgcgc gccgctctgg tctccggatg
     2281 cgagccggat cgtcttcact tccgcttccc gggggattct cgatctgtac gagaagccgg
     2341 cgagcggcag cggagagccg cacctcgtcg tttcctcctt gagtgacaag ttcccgaccg
     2401 actggacgcg cgacggacgg ttcctcattt accactcgtt cggaagcggg agcagcacgt
     2461 gggatatctg ggcagcgccg accgacggag gaaaacctct cgcgctcttc gcgtcgaagt
     2521 tcaccgaggc gcatggccgg gtttctccgg acgggcgctg gatcgcgtac gcctccgacg
     2581 agtcgggccg cttcgagatc tacgtgactc agttcccgga gaagcgcggc cgctggcaga
     2641 tctccacgac cggcggcagc cagccgtggt ggagaggcga tggaaaggag ctgttctatc
     2701 tcggccccga acaaaccctc atgagcgtcg ctgtcaggcc cggggatgcg ttcgaggctg
     2761 ccgtcccgac ctccctcttc aaggcgaact tcccgaccct ggtaccggcg ttctggccga
     2821 actactgcgc gaccgcggac ggtcagagat tcctcatcac cgagctggtt gccgaggtcg
     2881 tggcatcccc gatcaacgtc gtgctgaact ggaccgccgc gctcaagaaa tagggtcagg
     2941 tctccacttg ccacatcact tcgaccccgc cgcgaccgtc gccttgagct cctcgatcga
     3001 tcgcacgacg ccgccccgca cgacgtagcg caccttccgg aagttcgcaa cgtcggccgt
     3061 cggttcgccg ccgacgatca gcaggtccgc cgactttccc ttctcgaccg tgccggtctc
     3121 cttctccatc cccatcgccg ccgcgccttc gcgcgtcgag gcgacgatca cctgcatcgg
     3181 cgtcattccc gcggactgca tcgcctccat ctccgcgtag accgaggggc cgtgcagcgt
     3241 cagcggattc ccggcgtccg ttcccattgc gatcgggatg ccggcgtcca cgacgcgctt
     3301 caggttcgcc ttgccgatct gctccgcgcg caccgcccgc tcctcctgcc tggtcacctg
     3361 gaccgcgtat ccctcgggcg tcgggaccct cgcggtctcg gcgaccttcg cgagcgtctc
     3421 cgcgtcgacg catccgttcg ggtcgtccac ggcgggaggt ttcttcgacg agactccctg
     3481 cgccatccgg acgtaaccgc cgaacaccgt cagcgtcggg cagtagatcg tcccgttctt
     3541 cttcgcgagc gagaggaact cctcgtccac cggcaggtcg atgacgctgt gcacgagcac
     3601 gcccgctccg gctctcagcg cgactttcgc ttcggcgagt cccgtcgcgt gcacgatgag
     3661 cggcagcccg gccttccgcg cttcctctcc cgccgcgagc accacgggcg cggacttctc
     3721 gaccgggagc tccggccgca cgatgtacca gaccttcacg gcttcggcgc cgaccgactt
     3781 caggtacgcg acaccgttgc gcgccgactc cgcgtccttg agcaggatga actgccgctc
     3841 cgccggcagg ttcaaccagt gatcgagggt cgaaagcaga ggtcccgcgg cgccgacccg
     3901 cggcgcccgc gtgtcttggc gttcgcggtg cgccatcgcg accgtccacg gatatccgcc
     3961 gacgtcgaag accgtcgtga tgcccgagca gagctgggat cggaagaatc gatcggggtg
     4021 ttccttcagg tccgcctcga ccttctcgta ggggtgcgtg ctgcggacgt cgagggcgtc
     4081 cggccggccg tcggcccaac cggtctggga gaagtggacg tgcgcgtcga cgagcccggg
     4141 agtcacccac atcccgcgcg cgtccacgac ggtcacgcct cccggaatcg cgcaacgccc
     4201 cgcgcagtcg atctttccgc cgcgcagaac gaccgtggcg ttcggcacgg gcgcggctcc
     4261 tgtcccgtcc acgagcgtcc cgccg
//
LOCUS       DASWAV010000437         6567 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U2665
            k141_63125, whole genome shotgun sequence.
ACCESSION   DASWAV010000437 DASWAV010000000
VERSION     DASWAV010000437.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960316
            Sequence Read Archive: SRR10260007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 6567)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6567)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:47:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,929
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6567
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_63125"
                     /isolate="SMAG_U2665"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.36444 N 132.31889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2081)
                     /locus_tag="VF958_09100"
     CDS             complement(<1..2081)
                     /locus_tag="VF958_09100"
                     /EC_number="2.7.6.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR00691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0015970 - guanosine tetraphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HEX9149302.1"
                     /translation="MIRFEDILDRVETYNPQSNQDLLRRAYVFSALEHKGQVRRSGEP
                     YLIHPLNVAAILAEMKADDVSIVVGLLHDVLEDTLTTKELIAQQFGEEVADLVDGLTK
                     IGKFSYVSREQEQAETFRKMILAMVSDLRVVLVKLADRLHNMRTLQYLPEEKRREIAR
                     ETLDIYAPIANRLGMGLVKGELEDLAFMYLEPETYARTAREVERQMKVSQSMIEKVRA
                     RLQAKLAEMAIAGEVTGRLKRYYSIAEKMRRREVDAAELYDILAFRIVTSETRDCYAA
                     LGLVHQLWRPVPGRIKDYIAMPKPNFYQSLHTTVMGEAAQPFEVQIRTREMDLIAERG
                     IAAHWKYKEGRLGPHADDGRFQWLRQLVDWQSDVSDPRQFLSSLKVDLYPDEIYTFTP
                     KGEVFAFPRGATPVDFAYRVHSDVGDRCVGARVNGKLVPLRNPLASGDIVEILTAPNQ
                     TPSRDWLSFVATSRARHKIRHFIQAQENERAVELGRRLLERELKKYRKTIKRLESEGK
                     LQGELANWGFSRVEDLYAALGYGKLAPRNLLERLIPVEELDRPEPSKPETAVSRVVRK
                     ILPFGAPDITVVGHNDLLASLAKCCNPVPGEKIVGYITRGRGVSVHSDSCPNVRNLLY
                     DSGRQIDVAWASEKGASYAIELDVVTDDRPGLLADLTQAIAGEGSNILRIEARSSEDR
                     KGYVAVSLETS"
     gene            complement(2161..3399)
                     /gene="secF"
                     /locus_tag="VF958_09105"
     CDS             complement(2161..3399)
                     /gene="secF"
                     /locus_tag="VF958_09105"
                     /inference="COORDINATES: protein motif:HMM:TIGR00966.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecF"
                     /protein_id="HEX9149303.1"
                     /translation="MELFRQTNIDFLRYKWWAIGASWLMIAIGLFGIFGLKGLKFGID
                     FAGGTQIAVQFPDRPDLDRLRKILDSASLGDVGLQRYGEPEKNQVLIRVQQQKKEGRD
                     VSHDLQNALRASLQPGADAAKLDLNREGKDVIAVRLAAADPDRVSGRPDVVAVQYYGA
                     VAERVISYRSEAGILRSAADLDKVPDVSPAVRAWLKANSYTGPFVLLSAENVGPQVGA
                     DLRKKALLAVVWSIAGMLAYIAIRFRSLPFGVGAVVALIHDTLITVGLLALFGREFNL
                     VVVAALLTLVGYSVNDTVVVYDRVRENQRTPKKEALESVINRSINQTLSRTVLTSGAT
                     MLVVIALFFLGGEVLNTFALTLIIGIVVGTYSSIYVAAAIVVIWKDLKGKRTLQAVPA
                     VKPAAPPPPAAAKKKKSGRR"
     gene            complement(3409..5019)
                     /gene="secD"
                     /locus_tag="VF958_09110"
     CDS             complement(3409..5019)
                     /gene="secD"
                     /locus_tag="VF958_09110"
                     /inference="COORDINATES: protein motif:HMM:TIGR01129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HEX9149304.1"
                     /translation="MSKRLRWRVLLILLVIFGMIAGYIAMGYSNARREWTGTGKPPLS
                     QALKKGIKLGLDLRGGIHLVLQVNTADALKAERDDAAELLQAQAREQGITLGAVEFGS
                     ERAFAVAVTPQTDERKLEDTARRFLPDWTVSTAGGKWTFTLKDLARRQTEDNAVAQAV
                     ETIRNRIDEFGVAEPLIVQQGTDRILVQLPGIDDPKRVKDLIKSTAFLELKLVSGGPM
                     ADRTALLAPHGGQVPGNMELVEGAPAAGQREQVWYLVQKAAVITGRDLKNARPTQRQL
                     GGNAVTFFLNAAGAEKFASATGANVGKQLAIVLDKRVKSAPTINEQIHDQGEITGSFT
                     PEEANDLALVLRAGALPAGLTYLEERTVGPSLGLDSIKKGVTASIAGALFVFAAVVVY
                     YRLAGLNAVLALILNAVLLLGAMALFNATLTLPGIAGFILTIGMAVDSNVLIFERIRE
                     ELRAGRAPKTSIENGFSKAFATIIDTHLTVIISAIFLFQFGTGPVKGFAVTLILGLLI
                     SVFTAVFVSHTIFELMYGRRQHIDALSI"
     gene            complement(5086..5436)
                     /gene="yajC"
                     /locus_tag="VF958_09115"
     CDS             complement(5086..5436)
                     /gene="yajC"
                     /locus_tag="VF958_09115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004072717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HEX9149305.1"
                     /translation="MTAQLFDLLQSAPPTGPGGGLIQFLPMVFIFVIFYFLLIAPMRK
                     RQKRQQEMLTQLKKGDEVVTSGGIFGRIAAFDESHGAVILQIGENVKVKVLRSAIASP
                     ASEPEAATPTTPGS"
     gene            complement(5517..>6567)
                     /locus_tag="VF958_09120"
     CDS             complement(5517..>6567)
                     /locus_tag="VF958_09120"
                     /EC_number="3.4.13.19"
                     /inference="COORDINATES: protein motif:HMM:NF013413.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070573 - metallodipeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="dipeptidase"
                     /protein_id="HEX9149306.1"
                     /translation="EGGVTAPFLAAYVSSAYAKSGKSAKKALEFIDLIHSLVDSHPKD
                     LVFADSVAGIRAAKKQGKIAVLIGIEGGHAIEDSLGALSAFYRLGARYMTLTHTNTND
                     WADSAGSFFSFDFNPTRTAVHNGLTDFGREVVLEMNRLGMLVDVSHVSDKTLADVLDV
                     SIAPVFASHSSCRAIADMPRNLTDDQIRSIGATGGVVMVNVSSTFLDQKVVDDFKARK
                     AALAPKAAEIAERDKDDPAKRDAETSALLDNLQRPRAAWTAAVDHIEHVMKVAGRGAV
                     GLGSDFDGIDDPPDGLEDVSKLPVITEELLRRGHSEDEVRGVLGENFLRFWERAEAAR
                     SKVPPRKGPLPFSKP"
BASE COUNT         1102 a         2122 c         2170 g         1173 t
ORIGIN      
        1 tcggacgtct cgagtgacac ggcgacatac cccttccgat cttcggacga ccgggcctcg
       61 atccgcagga tgttcgatcc ctctccggcg atcgcctggg tcaggtcggc gaggagaccc
      121 ggccggtcgt cggtgacgac gtcgagctcg atggcgtagg acgctccttt ctcgctcgcc
      181 cacgccacgt cgatctgccg ccccgaatcg tagagaaggt tgcgtacgtt cgggcagctg
      241 tcggagtgaa ccgacacacc ccggccccgc gtgatgtacc cgacgatctt ctcgcccgga
      301 acggggttgc agcatttcgc cagcgacgcg aggagatcgt tgtgcccgac cacggtgatg
      361 tcgggagcgc cgaacgggag gatctttcga acgacccgcg aaaccgccgt ctccggcttc
      421 gagggttcgg gccggtccag ctcctcgacc gggatgagcc tctcgagaag atttcgggga
      481 gcgagcttgc cgtacccgag cgcggcatag aggtcttcga ccctcgagaa accccagttg
      541 gccagctcgc cctgcagttt cccctccgac tccaggcgct tgatcgtttt cctgtatttc
      601 ttgagctcgc gctcgaggag ccgccgcccg agctcgaccg cgcgctcgtt ctcctgcgcc
      661 tggatgaagt gccggatctt gtgccgcgcc cggctcgtgg cgacgaagga cagccagtcg
      721 cgggagggcg tctggttggg cgccgtcagg atctcgacga tgtcgccgct cgcgagcggg
      781 ttgcgcaggg ggaccagctt gccgttgacg cgcgcgccga cgcagcggtc gccgacgtcg
      841 gagtggactc gatacgcgaa gtcgaccggc gtcgcgccgc ggggaaacgc gaagacctcc
      901 cccttcggag tgaacgtgta gatctcgtcg gggtagaggt cgaccttgag cgaagagagg
      961 aactggcgcg ggtcggagac gtcgctctgc cagtccacga gctgccgcag ccactggaac
     1021 cgcccgtcgt ccgcatgggg ccccagccgg ccttccttgt acttccagtg cgccgcgatt
     1081 ccccgttcgg cgatgagatc catctcccgg gtccggatct gcacctcgaa cggctgcgcg
     1141 gcctccccca tcaccgtcgt gtgcagggat tggtagaagt tcggcttcgg catcgcgatg
     1201 tagtccttga tccggccggg aacgggcctc cacagctggt ggaccagacc gagcgccgcg
     1261 tagcagtcgc gtgtctccga ggtgacgatg cggaacgcca ggatgtcgta gagctccgcg
     1321 gcgtcgacct cgcggcgccg catcttttcc gcgatcgagt agtaccgctt gagccgtccc
     1381 gtgacctctc cggcgatcgc catctcggcg agcttcgcct ggaggcgcgc gcggaccttc
     1441 tcgatcatcg actgagagac tttcatctgc ctctcgacct cgcgcgccgt tcgcgcgtag
     1501 gtctccggct cgagatacat gaaggcgaga tcctcgagct cccctttcac gaggcccatt
     1561 ccgagccggt tcgcgattgg cgcgtagata tcgagggtct cacgggcaat ctcgcgccgc
     1621 ttctcctccg ggaggtactg gagcgtccgc atgttgtgca gtcggtccgc gagcttgacg
     1681 agcacgactc gcaggtccga gaccatcgcc aggatcatct tgcggaaggt ctccgcctgc
     1741 tcctgctcgc gggagacgta ggagaacttc ccgatcttgg tgagcccgtc cacgaggtcg
     1801 gcgacttcct ccccgaactg ctgagcgatc agctccttcg tcgtcagagt gtcctcgagg
     1861 acgtcgtgga gaaggccgac gacgatcgag acgtcgtccg ccttcatctc cgcgaggatc
     1921 gccgcgacgt tcagcgggtg aatgaggtac ggctcccccg agcgccggac ctgccccttg
     1981 tgctccagcg cggagaacac ataggcgcgc cgcaggaggt cctggttcga ctgcgggttg
     2041 taggtctcga cgcggtcgag gatgtcctcg aagcggatca tggcggagcc tagattaacg
     2101 ccgaggggga ggcgtcggaa atcgggaatc gaaggagagc gattcccgct cgattatcga
     2161 ttagcgcctg ccggacttct tctttttcgc ggctgcgggc ggagggggcg ccgccggctt
     2221 caccgccggc acggcctgga gcgtacgctt gcctttcagg tccttccaga tcacgacgat
     2281 cgcggccgcg acgtagatcg atgagtacgt cccgacgacg atcccgatga tgagcgtgag
     2341 cgcgaacgtg ttcaggacct ctcctccgag gaagaagagc gcgatcacga cgagcatcgt
     2401 cgcaccggag gtgaggacgg tccgggacag tgtctggttg atcgagcggt tgatcaccga
     2461 ctcgagggct tccttcttgg gcgtgcgctg gttctcgcgc acgcggtcgt acacaacgac
     2521 ggtgtcgttc acggaatagc cgacgagggt gaggagcgcc gcgaccacca cgaggttgaa
     2581 ctcgcgcccg aaaagagcga ggagtccgac ggtgatcaac gtgtcgtgga tcaaggcgac
     2641 caccgcgccg acgccgaagg gcagagaccg gaagcggatc gcgatgtagg cgagcatgcc
     2701 ggcgatcgac cagacgaccg cgaggagcgc cttctttcga aggtcggcgc cgacctgcgg
     2761 tccgacgttc tccgccgaca gcagaacgaa aggccccgtg taggagttcg ctttcagcca
     2821 ggcccgcacg gccggcgaga cgtcgggaac cttgtcgagg tccgcggcgg agcgaaggat
     2881 gcccgcctcc gaccgatagg agatgactcg ttcggcaacg gcgccgtagt actggacggc
     2941 gacgacgtcg ggccgccccg aaacgcggtc cggatccgcg gcggcaagac gcacggcgat
     3001 cacgtccttg ccctcccggt tcaggtcgag tttcgcggcg tccgctcccg gctgcagact
     3061 cgcccggagc gcgttctgca ggtcgtggga gacgtcgcgc ccctccttct tctgctgctg
     3121 tacgcgaatc agcacctggt tcttctcggg ctcaccgtag cgctgcaggc cgacgtcgcc
     3181 gagacttgcc gagtcgagga tcttgcgcag gcggtcgagg tcgggccggt cgggaaactg
     3241 gacggcgatc tgcgttcccc cggcgaagtc gatcccgaac ttcagaccct tcaggccgaa
     3301 gatgccgaag aggccgatcg cgatcatcag ccaggaggcg ccgatcgccc accacttgta
     3361 cctgagaaag tcgatgtttg tctggcggaa cagttccatt cgcgtctctt agatcgaaag
     3421 ggcgtcgatg tgctgccgcc gcccgtacat cagctcgaag atcgtgtgcg atacgaagac
     3481 ggcggtgaaa accgatatga gaagtccgag aatcagggtg accgcgaatc ccttgaccgg
     3541 ccccgttccg aactggaaca ggaagatcgc cgagatgatg acggtcaggt gcgtgtcgat
     3601 gatggtcgcg aaagccttcg agaagccgtt ctcgatcgaa gtcttgggag cgcggccggc
     3661 ccgcaactcc tcgcggatcc gctcgaagat gaggacgttc gagtccacgg ccatgccgat
     3721 cgtcaggatg aagcccgcga tcccgggcag cgtcagcgtg gcgttgaaaa gagccatcgc
     3781 gccgagcagc aggaccgcgt tcaagatgag cgcgagaacc gcgttcaacc ccgcgagccg
     3841 gtaatagacg acgacggcag cgaatacgaa gagagcgccg gcgatcgaag ccgtcacgcc
     3901 cttcttgatc gagtcgaggc caagcgacgg accgacagtt cgctcctcga gataggtgag
     3961 gcccgccggc agcgcaccgg cgcgcaggac gagcgcgagg tcgttcgcct cctccggcgt
     4021 gaagctgccg gtgatctcgc cctggtcgtg gatctgctcg ttgatcgtcg gcgccgactt
     4081 gactcgcttg tcgagaacga tcgcgagctg cttgccgacg ttcgcgcccg tcgccgaggc
     4141 gaacttctcc gcgcccgcgg cgttcaggaa gaacgtcacg gcattgccgc cgagctggcg
     4201 ctgcgtcggc ctcgcgttct tcaggtcccg ccccgtgatc acggccgcct tctggaccag
     4261 gtaccagacc tgttccctct gtccggcggc gggcgcgccc tcgacgagct ccatgttgcc
     4321 cggaacctgg ccgccgtgcg gcgcgaggag cgcggtgcgg tcggccatcg gtccaccgga
     4381 cacgagcttc aactcgagga acgccgtgct cttgatcagg tccttgacgc gcttcggatc
     4441 gtcgatgccg ggaagctgga cgaggatgcg gtccgttccc tgctgcacga tcaggggctc
     4501 ggcaacgccg aactcgtcga tgcggttgcg gatcgtctcg accgcctggg cgaccgcgtt
     4561 gtcttcggtc tggcgacggg cgaggtcctt cagggtgaag gtccacttgc cgccggccgt
     4621 cgaaacggtc cagtccggga ggaagcgccg cgcggtgtcc tcgagcttcc gctcgtcggt
     4681 ctggggggtc accgcgaccg cgaacgcgcg ctcgctcccg aactcgaccg cgccgagcgt
     4741 gatcccctgc tcacgcgcct gtgcctggag gagctcggcc gcgtcgtcgc gctccgcctt
     4801 gagggcgtcg gccgtgttca cttgaagaac gaggtggatg ccgccccgca ggtcgagccc
     4861 cagcttgatg cccttcttca gggcttgcga cagcgggggc ttccccgtgc cggtccactc
     4921 ccggcgggcg ttggaatatc ccatggcgat gtaccccgcg atcattccga agatgacgag
     4981 aaggatcagg agtacccgcc agcgaagtct cttggacaaa tcttcctccc ggaaaaagaa
     5041 ccgtggagtc taacggaagt ggacgtcagt cgccagctgc ggcgctcagc tgccgggggt
     5101 cgtcggcgtt gctgcctccg gttcggaggc cgggctcgcg atggcgctcc gcaggacctt
     5161 caccttcacg ttctcgccga tctgcaggat cacggcgccg tgggactcgt cgaacgccgc
     5221 gatccggccg aagatgccgc cgctcgtgac gacctcgtcc cccttcttca gctgcgtgag
     5281 catctcctgc tggcgcttct ggcgctttcg catcggcgcg atcaacagga agtagaagat
     5341 gacgaagatg aagaccatcg ggaggaactg gatgagaccg cctcccggcc ccgttggagg
     5401 ggcagactga agcaggtcga aaagctgtgc ggtcataggc gggggatcat atcggagacg
     5461 ggaatcggga atcgacaatc gaaaggaccc ggcctcctcg gttaccgatt tccgatttac
     5521 ggcttggaga acggcagtgg acccttcctg gggggaacct tcgaccgcgc cgcttcggcc
     5581 ctttcccaga acctcaggaa gttctctccg aggacccccc ggacttcatc ttcggagtgg
     5641 ccgcgccgca ggagctcttc ggtgatgacc ggcagcttcg agacgtcctc caggccatcc
     5701 ggcggatcgt cgatcccgtc gaagtcgctt cccagcccca cggcgccccg ccccgccacc
     5761 ttcatcacgt gctcgatgtg atcgaccgcc gcggtccagg ccgctcgcgg gcgctgaagg
     5821 ttgtcgagaa gggccgatgt ttccgcatcc ctcttcgcgg ggtcgtcctt gtcacgctcc
     5881 gcgatctccg cggccttcgg ggcgagcgct gccttgcgtg ctttgaagtc gtccaccacc
     5941 ttctgatcga ggaaagtcga gctcacgttg accatgacga ctcctccggt cgccccgatc
     6001 gaacggatct ggtcatcggt caggttgcgc ggcatgtccg cgatcgcccg gcacgaggag
     6061 tgggaggcga agactggcgc gatcgagacg tcgagaacgt cggccagcgt cttgtccgag
     6121 acgtgcgaga cgtcgacgag catgcccagc cggttcatct cgaggacgac ctcgcgcccg
     6181 aagtctgtca gcccgttgtg gacggcggtc ctcgtcgggt tgaagtcgaa agagaagaag
     6241 gaccccgccg agtccgccca atcgttcgtg ttggtgtggg tgagcgtcat gtatctggcc
     6301 ccgagtctgt aaaacgcgga gagcgcgccg agggagtcct cgatcgcgtg gccgccctcg
     6361 atcccgatga gcacggcgat ctttccctgc ttctttgccg cgcggatgcc ggcgacggaa
     6421 tcggcgaaca cgagatcttt cggatgcgag tcgacgagcg agtgaatgag gtcgatgaac
     6481 tcgagagcct tcttcgccga cttccccgat ttcgcgtacg cggacgacac ataggcggcg
     6541 aggaacggcg ccgtcactcc cccctcc
//
LOCUS       DASWAV010000438         2979 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U2665
            k141_63165, whole genome shotgun sequence.
ACCESSION   DASWAV010000438 DASWAV010000000
VERSION     DASWAV010000438.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960316
            Sequence Read Archive: SRR10260007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2979)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2979)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:47:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,929
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2979
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_63165"
                     /isolate="SMAG_U2665"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.36444 N 132.31889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..737)
                     /locus_tag="VF958_09125"
     CDS             complement(<1..737)
                     /locus_tag="VF958_09125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9149307.1"
                     /translation="MNGYPAHRIASSGLFSLFSLIALALGIGEARLLALATGGSLAAL
                     FVFTRGSFRDALGPFASSLATLADLALLFCALSLTGGFRSPYGLLLPAGVALAWHLEG
                     RSAARFYAAVSLLGVAALVTLGGGRLPPAAELFPLIATGLAAPALLAALEAMGSERSV
                     SRAVRAAGEEPARSAGVASELPPAERHGSDEILHDLKSPLSVARVYTDLIAEGARRGE
                     LPNAEHLANLSREIDLAERMVGGTSAVP"
     gene            complement(756..1850)
                     /locus_tag="VF958_09130"
     CDS             complement(756..1850)
                     /locus_tag="VF958_09130"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HEX9149308.1"
                     /translation="MDRERIALSIARLARLGRQRDFSFERFHKALESVAHPEEKAEVL
                     RRLLTEWTGLPHNVRDARERWEKVERIAGKMKNALGPPVSLQTALLHYFHSVEALLRE
                     PRLVSEKDLSVLRVNAITDPLTGLYNRRFLLDHLNREIARAERGDGIVSIVLLDLKGF
                     KAINDRFGHPMGDTVLVKTARIIRDLLRAVDAGCRWGGDEFVVVLPATDMFSALTVAE
                     RIRSKLADTTLPPGSGIHVGLHYGVASYPADGKTVDFLLKVADLRLYHCREQSAFAGP
                     ERRHYPRFPINDTSVRLPPNGRKRPWTAPLLEVSYGGLAFRARRSEKWPSRGKGEILR
                     LPLERRPVRIRVVNSARLPGGAVRVGCAYV"
     gene            complement(1889..2380)
                     /locus_tag="VF958_09135"
     CDS             complement(1889..2380)
                     /locus_tag="VF958_09135"
                     /inference="COORDINATES: protein motif:HMM:NF024803.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HEX9149309.1"
                     /translation="MKFNSVISHAFSTAQVARLTGLTPRQLDHWDRLGFLKPSLQKAC
                     GYGSMRRYSFEDVVRLRVAARLRSAGVGLARLRRCEEALRRLGGETNDLGGARLLVAG
                     SRVLWARSDQEVVDLLKEGQLVLVFSLDDAVQETAGAVARLNRENARDPDLSQQSRGK
                     QIF"
     gene            2622..>2979
                     /locus_tag="VF958_09140"
     CDS             2622..>2979
                     /locus_tag="VF958_09140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9149310.1"
                     /translation="MKRPMTFLAIVVAALLAAPVFAAQPPSSGPHFNLNIIGFANCTM
                     TTDGVYPDCFNGNTGPGGHVIFVPLKTSQTEDVCQTADISDPDNDGLVTLAALKKGVR
                     ILVTDGDQLEVLDKDAT"
BASE COUNT          552 a          977 c          982 g          468 t
ORIGIN      
        1 ggcaccgcgg acgtgccgcc gaccatccgc tcggccaggt cgatctctct cgaaagattc
       61 gccaggtgtt cggcgttcgg cagctcgccg cgccgggcgc cctccgcgat cagatcggta
      121 tagaccctcg cgaccgacag cggcgatttc agatcgtgga ggatttcgtc gctcccgtgg
      181 cgttcggccg gcgggagctc ggaggcgaca ccggccgatc gagccggctc ctcgccggcg
      241 gcgcggaccg cccgcgacac ggatcgttcg ctccccatcg cttcgagggc ggcgaggagg
      301 gccggggcgg caagacccgt cgcgatcagc ggaaagagct cggccgccgg cggcagccgt
      361 cctccgccga gcgtcacgag cgcggcgact ccgagaagag agaccgcggc gtagaagcgc
      421 gcggcacttc gtccttcgag gtgccacgca agggcgaccc cggccggcag gagaaggccg
      481 taaggactcc ggaaaccgcc ggtgagagaa agcgcgcaaa agagaagtgc gagatccgcc
      541 aacgtcgcga gggaagacgc gaaaggaccg agcgcgtcgc ggaagctgcc gcgcgtgaac
      601 acgaaaagtg ccgcgagcga gcccccggtc gcgagcgcga gcagccgggc ctcgccgatg
      661 ccgagcgcga gagcgatcag cgagaagagc gagaagaggc ccgacgaggc gatccggtga
      721 gcggggtagc cgttcaccgg acgcggttcc gcggatcaga catatgcaca gcccacccgg
      781 accgctcccc cgggaagcct ggccgaattc accactcgga tgcgcacggg tcgccgctcc
      841 agtggcagac ggagaatttc gccctttccg cgcgacggcc acttttcgct gcgccgcgcg
      901 cggaacgcga ggccgccata gctgacctcc aggagcgggg cagtccacgg ccgtttccga
      961 ccattcggcg ggaggcgcac gctcgtatcg ttgatcggga agcgcggata gtggcgccgt
     1021 tccggacccg cgaaggccga ctgctcgcgg cagtggtaga gccgcaggtc ggcgaccttg
     1081 agcaggaagt ccacggtctt gccgtccgcg ggatacgacg ccaccccgta gtgcaggccg
     1141 acgtggatgc ccgatcccgg cgggagtgtc gtgtcggcga gcttcgagcg gatccgctcg
     1201 gccacggtga gagccgagaa catgtccgtc gcagggagca cgacgacgaa ctcgtctcca
     1261 ccccagcggc aaccggcgtc gaccgcgcgc agaagatcgc ggatgatccg ggcggttttc
     1321 acgagcacgg tgtcgcccat cgggtgtccg aagcggtcgt tgatcgcttt gaagcctttc
     1381 aaatccagga gcacgatcga cacgatcccg tcgccgcgct ctgcgcgcgc gatctcccgg
     1441 ttcaagtgat cgagcaggaa acggcggttg tacaagccgg tcagcggatc ggtgatcgca
     1501 ttcacgcgca gcacggagag gtctttctcc gatacgagtc gcggctctcg gaggagcgcc
     1561 tcgacgctgt ggaagtagtg gaggagcgcc gtctgcaggc tcacgggcgg accgagggca
     1621 tttttcatct tcccggcgat ccgctcgacc ttctcccagc gttcgcgggc gtcgcggacg
     1681 ttatggggaa ggcccgtcca ttcggtgagc aggcgtcgga gcacctccgc cttttcttcc
     1741 ggatgcgcaa cggattcgag cgctttgtgg aaacgctcga acgagaagtc gcgctggcgt
     1801 ccgagccgcg cgagtctcgc gatggagagc gcgatcctct cccgatccaa ccttccccct
     1861 cgcccccggc tccaacgagc cgggactttc agaagatctg tttcccccgc gattgctgcg
     1921 aaaggtccgg gtcgcgcgcg ttctcgcggt tcaggcgggc cacggcgccc gcggtctcct
     1981 gcaccgcgtc gtcgagcgag aagacgagca cgagctgtcc ttctttcagg agatccacga
     2041 cctcctggtc cgagcgcgcc cagagcactc gcgatcccgc aacgagtagc cgcgcgccgc
     2101 cgaggtcatt cgtttctccg ccgaggcgcc ggagagcctc ctcgcatcgg cgaagccgcg
     2161 cgaggccgac tcccgccgac cgcagccgcg cggcaacgcg caacctcacc acgtcttcga
     2221 acgagtagcg gcgcatcgag ccgtagccgc acgccttctg gagcgacggt ttcaaaaagc
     2281 ccagccgatc ccagtgatcg agctgacgcg gagtcaatcc ggtcagccga gcgacctgcg
     2341 ccgtcgaaaa ggcgtgcgaa atcacactgt tgaatttcat ggatgaagtc tgcggaatcg
     2401 tagcacgcga cctctcgcaa atcttcgaga aacaggggtt ttgcctctat ccgcgacttt
     2461 ggtagtattc gactaaagtc gtaatacacc ccgggtcgga ggagggggtg actcctgacc
     2521 gccgagggtt ctgtggagtg gaaaggagga acgccggaca gcaaggattc ccaagtctcg
     2581 tcgactgaac gcatctctca atcacgagga gctaaattcg aatgaaacgt cccatgacct
     2641 ttctcgcaat tgttgtcgct gccctgctcg ccgcgccggt cttcgccgcg cagcccccgt
     2701 catcggggcc gcacttcaat ctgaacatca tcgggtttgc caactgcacc atgacgaccg
     2761 acggcgtcta cccggattgt ttcaatggaa ataccgggcc gggcggccac gtcatctttg
     2821 ttccgctgaa gacgtcgcag acagaggacg tctgtcagac cgccgacatc tcggatcccg
     2881 acaacgacgg tctcgtgaca ctcgcggcgc tgaagaaggg cgtgaggatt ctcgtcacgg
     2941 acggcgacca gctcgaagtt ctggacaagg acgcaacgg
//
LOCUS       DASWAV010000439         1892 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U2665
            k141_63184, whole genome shotgun sequence.
ACCESSION   DASWAV010000439 DASWAV010000000
VERSION     DASWAV010000439.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960316
            Sequence Read Archive: SRR10260007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 1892)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1892)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:47:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,929
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1892
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_63184"
                     /isolate="SMAG_U2665"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.36444 N 132.31889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1892
                     /locus_tag="VF958_09145"
     CDS             <1..>1892
                     /locus_tag="VF958_09145"
                     /inference="COORDINATES: protein motif:HMM:NF019334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HEX9149311.1"
                     /translation="ADVVTLGRLGQGDCRIRLGESAAGMKLLDEVMVAVTADEVYPLL
                     SGLVYCAVISACHSTFDLRRAQEWTAALTRWCESQAGLVAYRGVCLVHRAEILQLQGE
                     WPDAADEVRQACERLSQPPGHASVGSAHYVRGELHRLRGEFAEAEEAYRQASLAGRKP
                     DPGLAELRLAQGRVEIATAAIRRVFEETTDRRMRSRILPAYVDILLAAGDVPGARQGA
                     EELSEIAAALDAPFLRAVAARAKGAVLLAEGDVRTALGTLKDAAGELLDIDVPYETAR
                     VRVIVALACRQLGDEETANMELDAARETFEQLGAAPDLDSVEQLRRAGASIASFPVVA
                     RKAITLGPGARLGPYEIVASVGAGGMGEVFRARDSRLGRDVAIKILTVANAGDPDSVR
                     RFEKEARAVASLSHPNVVPLFDVGDQDGVRYAVTEFVAGETLRERLKRGPMASGEAAE
                     IAAQIADGLSAAHEKGFVHRDVKPENVVLARPDFARILDFGLAKRNPEMAGLSVSEEA
                     PTQSAILTQPGIIAGTVGYMSPEQVRGETVDARSDIFSLGVVLWEMLTGRRPFRGDSP
                     VDTLGAILRDEPPPDPALANLPPEFDRALRRALEKRREDRYPSAADLARDLRQLARDG
                     PVGGSTR"
BASE COUNT          288 a          643 c          683 g          278 t
ORIGIN      
        1 gcggacgtcg tgacgctcgg acggttgggt cagggcgatt gccgcatccg cctcggcgag
       61 tccgcggcgg gaatgaagct cctggacgaa gttatggtcg ccgtcacggc cgacgaggtt
      121 taccctctcc tcagcgggct cgtctactgc gccgtgatca gcgcatgcca ctcgaccttc
      181 gacctgcgcc gggcccagga gtggaccgcc gcgctcacgc ggtggtgcga atctcaagcg
      241 ggcttggtcg cctaccgcgg agtctgcctc gtgcaccgcg ccgagatcct gcagcttcag
      301 ggtgagtggc ccgacgccgc cgacgaagtc cggcaggcgt gcgagaggct gtcgcagccg
      361 cccggccacg catccgtcgg cagcgcccac tacgtgagag gcgagctgca ccgcctccgc
      421 ggagagttcg cggaggccga agaggcctac cgtcaggcaa gtctcgccgg acggaagccg
      481 gacccgggcc tcgcggagtt gcggctcgcg caggggcgcg ttgagatcgc gactgcggcg
      541 attcgacggg tgttcgagga gaccacggac cggcgcatgc ggtcccggat ccttccggcc
      601 tacgtcgaca tcctgctcgc cgcgggagac gtccccggcg cgcgccaggg ggcagaggag
      661 ctctccgaga tcgccgccgc gctcgacgcg ccgttcctgc gggccgtggc ggcccgggcg
      721 aaaggcgcgg tgctgctcgc cgagggcgac gtacggactg ccctcggcac cctcaaagac
      781 gcggcgggcg agctgctcga catcgacgtt ccgtacgaga ccgcgcgcgt tcgcgtcatc
      841 gtcgccctcg cgtgccgcca gctcggcgac gaagagacgg cgaacatgga gctcgacgcg
      901 gcgcgcgaga cgttcgagca actcggcgcg gcgcccgacc ttgacagcgt cgagcaactc
      961 cggcgcgcgg gggcgtcgat cgcgtccttc ccggtcgttg cccgaaaggc gatcaccctc
     1021 gggcccggag cgcggctggg tccgtacgag atcgtcgctt cagtcggcgc gggcgggatg
     1081 ggcgaagtct tccgagcccg cgactcacgg ctcggccgcg acgtggccat caagatcctg
     1141 acggtcgcga acgccggcga ccccgacagc gtccgacgtt tcgagaaaga ggcgcgagcg
     1201 gtcgcctctc tttcccatcc gaacgtcgtt ccgctcttcg acgtagggga tcaggacggg
     1261 gttcgatatg cggtgaccga gttcgtcgcc ggcgagacgc tgcgggaacg attgaagcgg
     1321 gggccgatgg cttccggcga ggccgcggaa attgcggccc aaatcgccga cggactctcc
     1381 gccgcgcacg agaagggatt cgtgcaccgg gacgtcaagc cggaaaacgt ggtcctcgcc
     1441 cggccggact tcgcgcgcat cctcgatttc ggcctggcga agagaaaccc ggagatggcg
     1501 ggcttgtccg tctcggagga ggcgccgacc cagagcgcaa tcctgacgca gccggggatc
     1561 atcgcgggaa cggtgggata catgagcccg gagcaggttc gaggcgagac ggtggacgcc
     1621 cggtcggaca tcttctcgct gggggtggtg ctgtgggaga tgctcaccgg gcgccgaccg
     1681 ttccgcggtg actcacccgt cgatacactg ggcgcgatat tgcgggacga gccgcctccg
     1741 gatcctgcgc tcgcgaatct tccgcccgaa ttcgaccgcg cgctgcggcg cgctctcgaa
     1801 aaacgtcggg aggatcgtta tccctcggcc gccgacctcg cacgcgacct gcgccagctt
     1861 gcgcgcgacg gcccggtggg cggctcgacc cg
//
LOCUS       DASWAV010000440         2034 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U2665
            k141_63220, whole genome shotgun sequence.
ACCESSION   DASWAV010000440 DASWAV010000000
VERSION     DASWAV010000440.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960316
            Sequence Read Archive: SRR10260007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2034)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2034)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:47:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,929
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2034
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_63220"
                     /isolate="SMAG_U2665"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.36444 N 132.31889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..292)
                     /locus_tag="VF958_09150"
     CDS             complement(<1..292)
                     /locus_tag="VF958_09150"
                     /inference="COORDINATES: protein motif:HMM:NF025690.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4388 domain-containing protein"
                     /protein_id="HEX9149312.1"
                     /translation="MIPPFQGRLSTVGFADLLQWMELNRRSGRLIVMRGRDRRVIDWK
                     DGEIAYVSGSDPRNRFGVFLNRTRALPRATLHSLLARTFTSGTNLTRLILDGR"
     gene            446..1957
                     /locus_tag="VF958_09155"
     CDS             446..1957
                     /locus_tag="VF958_09155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014099056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016866 - intramolecular transferase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase family protein"
                     /protein_id="HEX9149313.1"
                     /translation="MPIEPFYGEPPPGEFPFTRGITPETYRSRLWTMRQYAGYGSARE
                     SNRRYRYLLEQGQTGLSVAFDLPTQMGYDSDHPLARGEVGKAGVAIATIEDMRLLTEG
                     LPLGQVSISMTINSTAPILLALLLSVARERGISWEELAGTVQNDLLKEYAARGTYVFP
                     PRPSLKIATDIFEFCGREVPRWNTISISGYHIREAGSTAVQEVAFTLANGIAYVEAAV
                     SRGLAVDSFGPRISFFFNAHSNFFEEVAKFRAARSLWAEIMRDRFGAKDPRSWRMRFH
                     TQTAGSTLTAQQPDVNVVRVALQALSAVLGGTQSLHTNAADEALALPTERSARLALRT
                     QQVIGFETGTADVADPLGGSALVEEWTEEIRGRARDLIEKIDTLGGAVAAIERGWIQR
                     EIEESAYQAQREIEERRRIVVGVNAFREEGGEATPVMTVDPEIERDQVDRLGAFRARR
                     DSRRSEVALSAVVREAREDRNLMPVLIEAVAAEATLGEIVSSLKTVYGEHRPG"
BASE COUNT          347 a          685 c          686 g          316 t
ORIGIN      
        1 gtcgcccatc gagaatgagc cgcgtcaggt tcgtgcccga cgtgaaggtc cgtgcgagaa
       61 gcgagtggag cgtcgctcga ggcaacgccc gcgtgcggtt caagaagacc ccgaaccggt
      121 tccggggatc ggaaccggag acgtacgcga tctcaccgtc cttccagtcg atgacgcgcc
      181 ggtctcgacc gcgcatcacg atcaagcggc cggatcggcg gttcaactcc atccactgga
      241 gcaggtcggc gaatcccacc gtcgagaggc gtccctgaaa gggaggtatc acagtgcgat
      301 tgtagagcgc ggcgttggct gcctttcacg gcggcgggtg tgcaacaatc gaagagtgac
      361 cgagaagacg gcgcgcccgc gaacggaatc gggccgtccg gggagcgtcc gggagcctcc
      421 gccacccggg cagaccagct ccgggatccc gatcgagccg ttctacggcg agccgccgcc
      481 cggcgagttt cctttcacgc ggggcatcac gccggaaacg taccggtcgc gcctctggac
      541 gatgcgccag tacgccgggt acggctccgc gcgggagtcg aacaggcgat accgctatct
      601 cctcgagcag ggccagaccg ggctctccgt cgccttcgat ctgcccaccc agatgggcta
      661 cgactccgat cacccgctcg cccgcggcga agtgggcaaa gccggcgtcg cgatcgcgac
      721 gatcgaagac atgcgcctcc tgacggaggg gctgccgctc gggcaggtct cgatctcgat
      781 gacgatcaac tcgaccgccc ccatccttct cgctctcctg ctgtcggtcg cacgggaaag
      841 gggcatctcc tgggaggagc tcgccggcac ggtgcagaac gaccttctga aggagtacgc
      901 ggcgcgcgga acgtacgtct tccccccgcg gccttcgctc aagatcgcga ccgacatctt
      961 cgagttctgc ggccgcgaag tcccccgctg gaacaccatt tcgatctcgg gctaccacat
     1021 ccgggaagcc ggctcgaccg cagtccagga ggtcgctttc acgctcgcga acgggatcgc
     1081 gtacgtcgag gccgccgtct cccggggtct cgccgtcgat tccttcggtc cccggatctc
     1141 tttcttcttc aacgcacact cgaatttctt cgaggaggtc gccaagttcc gcgcggcgcg
     1201 gtcgctctgg gccgagatca tgcgggaccg cttcggcgcg aaagatcccc ggtcctggcg
     1261 gatgcggttt cacacgcaga cggcgggatc gacgctgacg gcgcagcagc ccgacgtcaa
     1321 cgtcgtgcgg gtcgcgctcc aggctctctc cgccgtgctc ggcggaacgc aatcgctcca
     1381 cacgaacgcg gccgacgagg cgctcgcgct tccgaccgag cgatcggcgc ggctcgcgct
     1441 ccgcacgcag caggtgatcg gtttcgagac cggcaccgcg gacgtcgccg atccgctcgg
     1501 cggttcggcc ctcgtcgagg agtggacgga ggagattcgg ggccgcgcgc gcgacctcat
     1561 cgagaagatc gacacgctcg ggggcgccgt cgcagcgatc gagcgcggct ggattcagcg
     1621 ggagatcgag gaatccgcct atcaggccca gcgcgagatc gaggagcgac gtcggatcgt
     1681 ggtcggcgtc aacgccttcc gcgaagaggg gggtgaagcg acccccgtca tgaccgtgga
     1741 cccggagatc gaacgggatc aggtcgatcg cctgggcgct ttccgggcga ggagggacag
     1801 ccgacggtcg gaggtggccc tttccgcggt cgtacgcgag gcgcgcgagg accgcaacct
     1861 gatgccggtt ctcatcgaag cggtcgccgc cgaggcgacg ctcggcgaaa tcgtctcgag
     1921 cctgaaaact gtctacggcg agcaccgccc cgggtagtca cggggacagg tcccgcggac
     1981 ctgcacccgt tgcagtccac ttaagtccaa gcgccaaaag cgcttgtcga tgtg
//
LOCUS       DASWAV010000441         4641 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U2665
            k141_382305, whole genome shotgun sequence.
ACCESSION   DASWAV010000441 DASWAV010000000
VERSION     DASWAV010000441.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960316
            Sequence Read Archive: SRR10260007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 4641)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4641)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:47:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,929
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4641
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_382305"
                     /isolate="SMAG_U2665"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.36444 N 132.31889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            133..3234
                     /locus_tag="VF958_09160"
     CDS             133..3234
                     /locus_tag="VF958_09160"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX9149314.1"
                     /translation="MRPDWEKGIRGRLYALGRRVVPLSWRRAVRRKLAPEKLLGLRKP
                     AVDVPRFDFDPTEARPGRPDVVVLPVIAWSYRRQRPQHLAAALARRGRRVFYGSLGGS
                     GEPADLTGVERGVALFPIGGVRREDPADRRLEGATLDRAAESLARVRDRFDLREAALL
                     VQSPFWAPLAARLSERFGWKVVYDCMDEHGSFPANRSSALASAEEEIVSAADLVVATS
                     EPLLERLRTRRPDAVLLPNAADAELFGSLPDPAPEPGRLTVGYAGAVDDWFDFDLLAA
                     AARLRPNWRFEVVGGVEGRRPSPSRFPENVVFAGERPYSEMPAIRAKFDVEIIPFRLS
                     ALTHATDPVKLYEALAAGRGVVATPMRALAPFAERDLVRFAETPDEFVRQIASAAAAA
                     PGEIARRRAFARDNTWDVRAGELSRRLEELWPLVSIVIVTHDGFGWTRLCLESLDRRT
                     DWPRFEVVVADNASTDATREWLGDEERRGTLRAVMLPDNRGFAAGINAAAAASRGDYL
                     CVMNNDTVVTRGWLSALVRHLERDAGLGMVGPSTNEIANEAKVEVGYRELEHLEPWSR
                     NFTRANAGRRDELPMLAMFCTLLRRETFEKVGPLDERFEIGMFEDDDYARRLSAAGLT
                     LAVARDSFVHHAGRGSFRALPEEEYQRIYRENQRRFEEKWSAHPRVAVEQASFDEIAR
                     RAAATGALFVFPPTIGWDVALVQRPHHLARALAKLGFPVVFEEEPSTVPEAAAGALRS
                     EGDNLWISREPTPAEIPHRVFWSFAYNVPEKARLSDARLVYDVIDHPDVFPHPRRTLR
                     RNHDRALEIADAVFAVSQPLLTEVRQRRADAVYLPNGVEFARFSAPPDPTAVPEEMTR
                     SRSRGRPAAGYVGALARWVDSDLLAALAALRADWDFFVVGEALDDSFDRFATARPANL
                     YLLGPRPYATIPSILSALDAGLIPFRVGPEGSHASPIKLYEYLAAGLPVLSTPMPECE
                     LVVEVEVAASAAAFSDLLDRARVSRRSEDFRRRARERACQNDWSQRVATALAALSLSV
                     KIA"
     gene            3263..4108
                     /locus_tag="VF958_09165"
     CDS             3263..4108
                     /locus_tag="VF958_09165"
                     /inference="COORDINATES: protein motif:HMM:NF013245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX9149315.1"
                     /translation="MSAASTAASAAGAVYDSSEHRSRTLEEARALWKYRGLVQELIVR
                     DIKVRYKRSVLGIVWTMLAPLLNMVALTLVFSTLLKQQITNYPVYFMTGAIFWAFFSQ
                     STSSAAAQTQDSNEIAKRIYIPRSVFVAAAIGVGLVNLALSIVPLVLILLVTGFPIHA
                     TWAFVPVAVVITTLFAAGVGFWLFTFASRFSDIREMYQVIVQTWFFLTPIVYHPAIVP
                     PKFRFALWLNPLYYLVQTFRKPIYDGVLPSPGLIGASLALSLAVFLSGWAYFSNRAER
                     FAFLS"
     gene            4113..>4641
                     /locus_tag="VF958_09170"
     CDS             4113..>4641
                     /locus_tag="VF958_09170"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX9149316.1"
                     /translation="MPPVISVHRASVRYRIPRERIGSLKEYAIRRLKRRLFFDEFEAL
                     RDVSLEVNAGETVGVVGKNGAGKSTLFRLIARVMPPSAGRVVVFGRVAPLLELGLGFH
                     SELTGRENIMLQGTLLGFSRAEMRRRTPRIVEWAEIEEFIDAPIRTYSSGMAARLAFS
                     VATDVEPEILLVDEAL"
BASE COUNT          630 a         1652 c         1564 g          795 t
ORIGIN      
        1 ggatcgctcc gattcggtcc gccgcggaca ggctgcgaag cgtcggatgg agagtctcta
       61 cgggctcgaa tccgcgggcc gccgtcttgc cgaacacgcg gaccggctcc tcgcccggct
      121 cgccgcgcag ccatgagacc cgactgggag aaggggatcc gcgggaggct ctacgcgctc
      181 gggcggcgcg tcgtgccgct ttcgtggcgt cgcgcggtgc gccggaaact cgctcccgaa
      241 aagctgctcg gcctgcgaaa gcccgcggtc gacgtcccgc ggttcgactt cgacccgacc
      301 gaggcgcggc ccggccgacc cgacgtcgtc gtgctcccgg tgatcgcgtg gtcctaccgg
      361 cgtcagcgcc cgcagcacct cgctgcggcg ctcgcgcgcc gcggacggcg cgtcttctac
      421 ggatcgctcg gggggagcgg cgagcccgcg gatttgaccg gagtcgaacg gggggtcgcg
      481 ctgtttccga tcggcggcgt ccgtcgggag gaccccgcgg atcggcggct cgaaggggcc
      541 acgctcgacc gcgccgcgga gtcgctcgca cgggtgcgcg accgcttcga cctgcgcgaa
      601 gccgccctcc tcgtccagag ccccttctgg gcgccgctcg ccgctcgcct ctcggagagg
      661 ttcggctgga aggtcgtcta cgactgcatg gacgagcacg ggtcttttcc ggcgaatcgg
      721 tcgtccgcgc tcgcgtccgc cgaagaagag atcgtgagcg ccgccgacct cgtcgtcgcg
      781 acctccgaac cgctcctcga gcggctccgg acccggcggc cggacgcggt cctcctgccg
      841 aacgccgccg atgccgagct cttcgggtcg ctgcccgatc ccgccccgga gcccggccgg
      901 ctcaccgtcg gctacgccgg cgcggtcgac gactggttcg acttcgacct tctggccgcg
      961 gccgcgcgcc tccggccgaa ctggcgattc gaggtcgtcg gaggcgtcga ggggcggcgc
     1021 ccctccccgt cgcggttccc cgagaatgtc gtgttcgcgg gcgagcggcc ctattcggag
     1081 atgccggcga tccgcgcgaa gttcgacgtc gagatcattc ccttccgcct gtcggcgctg
     1141 acgcacgcga ccgatccggt caagctctac gaagctctcg ccgccggccg cggcgtggtc
     1201 gcgacgccga tgcgcgcgct cgcgccgttc gcagagcgag acctcgtccg cttcgccgag
     1261 actcccgacg agttcgtccg ccagatcgcg agcgcggcgg ctgcggcgcc gggagagatc
     1321 gcacgacgcc gagccttcgc gcgggacaac acctgggacg ttcgcgcggg ggagctctcc
     1381 cgccgactcg aggagctctg gccgctcgtc tcgatcgtca tcgtcacgca cgacgggttc
     1441 ggctggacgc ggctgtgcct cgagtcgctc gaccgtcgga ccgattggcc gcgcttcgag
     1501 gtcgtcgtcg cggacaacgc gtcgacggat gcaacccgcg agtggctcgg agacgaggag
     1561 cgccgaggca cgctgcgcgc cgtgatgctg ccggacaacc gcgggttcgc cgccggaatc
     1621 aacgccgcgg cggcggcgtc ccgcggcgat tacctctgcg tgatgaacaa cgacacggtc
     1681 gtgacgcggg gctggctctc cgccctcgtg cggcacctcg agcgcgacgc gggcctcgga
     1741 atggtcgggc cctccacgaa cgagatcgcc aacgaagcga aggtcgaggt cgggtaccgc
     1801 gagctcgagc atcttgagcc gtggtcgcga aacttcacga gagccaacgc cggccgccgg
     1861 gacgagctcc cgatgctggc catgttctgt acgctcctgc ggcgcgaaac tttcgagaaa
     1921 gtcggacccc tcgacgagcg gttcgagatc ggcatgttcg aggacgacga ctacgcgcgg
     1981 cggctgtcgg ccgcgggcct caccctcgcg gtcgcccgcg actctttcgt ccaccacgcg
     2041 ggacggggca gcttccgcgc gttgccggaa gaggagtacc agcggatcta ccgcgagaac
     2101 cagcggcgct tcgaggagaa gtggagcgcg cacccgcggg tcgccgtcga acaggcgtcg
     2161 ttcgacgaga tcgcgcggag ggcggcggcg acgggggcgc tgttcgtctt ccctccgacg
     2221 atcggttggg acgtggcgct cgtgcagcgc ccccaccacc tcgcccgggc gctggcgaag
     2281 ctcgggttcc cggtggtttt cgaggaggag ccttccacag tgccggaggc cgcggctggc
     2341 gcgctgcgtt cggaaggaga caatctctgg atctcccgag agccgacgcc cgcggagatt
     2401 ccgcatcgcg tgttctggtc cttcgcatac aacgtcccgg agaaggcgcg gctctcggac
     2461 gcgcggctcg tctacgacgt catcgatcat cccgacgtgt ttccccatcc gcggcgcacg
     2521 ctccggcgca accacgaccg cgcgctcgag atcgccgacg ccgtgttcgc ggtctcacag
     2581 ccgctcctga ccgaggtccg ccagcgccgt gcggacgccg tgtacctccc caatggcgtc
     2641 gagttcgcgc ggttttcggc cccgccggat ccgaccgccg tcccggaaga gatgacccgc
     2701 tcgcgctcgc gcggacgacc cgcggccggt tacgtcggag cactcgcccg gtgggtcgac
     2761 tcggaccttc tcgccgcgct cgcggcgctg cgggccgact gggacttctt cgtcgtgggg
     2821 gaggcactcg acgactcctt cgatcgcttt gcgaccgcgc ggccggccaa cctctacctg
     2881 ctcggtcccc ggccctacgc gacgattccg tcgatcctct ccgcgctcga cgccggactg
     2941 atcccgttcc gcgtcggccc cgagggctcg cacgcctcgc cgatcaagct ctacgagtac
     3001 ctcgccgcag gcctgccggt cctgtcgacg ccgatgcccg agtgcgagct cgtcgtcgaa
     3061 gtcgaggtcg ccgcgtctgc cgccgcgttc tccgatctcc tcgatcgggc ccgcgtttcg
     3121 agacggtcgg aggatttccg ccgtcgtgcg cgggagcgcg cttgtcagaa cgactggtcg
     3181 cagagggttg cgactgccct cgccgcgctt tctctctcgg ttaaaatcgc atgatgtccg
     3241 gacaaacccg gggccaggcg acttgagcgc ggcttcaacc gccgcctccg ccgccggagc
     3301 cgtctacgac tcgtcggaac atcgatcccg cacgctcgag gaggcgcggg cgctgtggaa
     3361 ataccggggg ctcgtccaag agctcatcgt tcgggacatc aaagtgcgct acaagaggtc
     3421 ggtcctcggg atcgtctgga cgatgctcgc gcccctgctc aacatggtcg cgctgacgct
     3481 cgtcttctcg acgctcttga agcagcagat caccaactat cccgtgtatt tcatgacggg
     3541 cgcgatcttc tgggcgttct tttcccagag cacgtcttcc gccgcggccc agacccagga
     3601 ctcgaacgag atcgccaagc ggatctacat cccgcgctcg gtcttcgtcg cggcggcgat
     3661 cggagtcggt ctcgtgaacc ttgcgctgtc gatcgttccg ctcgtcctga tcctgctcgt
     3721 gaccggcttt ccgatccacg cgacgtgggc attcgtgccg gtcgcggtgg tcatcacgac
     3781 tctgtttgcc gcgggcgtcg gcttctggct gttcaccttc gcgtcgcgct tctccgacat
     3841 ccgcgagatg taccaggtga tcgtgcagac gtggttcttc ctgacgccga tcgtgtacca
     3901 cccggcgatc gtgccgccga agttccgatt cgcgctgtgg ctgaaccctc tctattacct
     3961 cgtccagacg ttccgcaagc cgatctacga cggcgtactc ccgtcgcccg gcctgatcgg
     4021 agcttcgctc gctctgtcgc tcgctgtttt cttgagcggc tgggcctact tctcgaaccg
     4081 cgccgagcgg ttcgcgttct tgagctaacc ggatgccgcc agtcatctcc gtccaccgcg
     4141 cctccgtccg ctatcgcatc ccgcgggagc gcatcggttc gctcaaggag tacgcgatcc
     4201 gtcgcctgaa gcggcgcctg ttcttcgacg agttcgaggc gctacgcgac gtgagcctcg
     4261 aggtcaacgc cggggagacg gtcggggtcg tcggaaagaa cggcgcgggt aagagcacgc
     4321 tcttccggtt gatcgcccgt gtcatgcccc cctccgcggg ccgagtggtc gtcttcggaa
     4381 gggtcgcgcc actgctcgag ctcgggctcg ggttccacag cgagctgacc ggccgcgaga
     4441 acatcatgct gcagggcacg ctgctcggct tctcgcgcgc cgagatgcgg cggcgcaccc
     4501 cgcggatcgt cgagtgggcg gagatcgagg agttcatcga cgctcccatt cggacctatt
     4561 cgagtggaat ggccgcgcgg ctcgcgttct cggtggcgac cgacgtggag ccggagatcc
     4621 tcctcgtcga cgaggcgctc g
//
LOCUS       DASWAV010000442         2038 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U2665
            k141_745009, whole genome shotgun sequence.
ACCESSION   DASWAV010000442 DASWAV010000000
VERSION     DASWAV010000442.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960316
            Sequence Read Archive: SRR10260007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2038)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2038)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:47:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,929
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2038
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_745009"
                     /isolate="SMAG_U2665"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.36444 N 132.31889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1133
                     /locus_tag="VF958_09175"
     CDS             <1..1133
                     /locus_tag="VF958_09175"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HEX9149317.1"
                     /translation="TAYSSIEGASEAMREGAFHYIPKPVQNQEVLLTVRKGSEARRLT
                     EENRRLKEELSKRYGLGRIVGKSETMRKVFDLVRLAGPSKSTILVEGESGTGKELVAR
                     AIHTHSPRSGAPFVTVNSGSMPPDLLESNLFGHVRGAFTGAVANKKGLFEVAEGGSIF
                     LDEIGTVGLETQAKLLRVIQEKEFMRLGSVETQKADVRIIAATNVDLKKLVVEGRFRE
                     DLYYRLCVITIPIPPLRERREDIPLLADHFVRLYASENNKPISGLVPESMKALLDYDW
                     PGNVRELENAIERAVVLCSAGSIGLDLLPETVLQPGRGEVMVRLPENGSTYRDLIEEY
                     ERRLIRTALRRTGGVQKRAAELLKLKPTTLHEIIKRLEIRDQ"
     gene            1179..>2038
                     /locus_tag="VF958_09180"
     CDS             1179..>2038
                     /locus_tag="VF958_09180"
                     /inference="COORDINATES: protein motif:HMM:NF012471.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004181 - metallocarboxypeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M14 family zinc carboxypeptidase"
                     /protein_id="HEX9149318.1"
                     /translation="MRKVAFALLLLVALVPAAIADDDPLFSDRGIPSNYAPSQMDVRV
                     PTPEKVLGFKIGDRFATHAEVTAYVRAVAAAAPERVRVETYGRTPEGRELLVVVVTSP
                     ENHARFDELAAKIRALYSPSSAKPAAIPADLPVFVWFSYGVHGDEASSSDAAVATLYH
                     LAASRDANTAELLRRVVFTMDPMVNPDGRMRYLSWFTTAVNGEPDPNPDAREHHPSWP
                     RGRGNHFGFDLNRDWAWLTQPETRARIERYRKTPPQVHVDFHEMSPESSYFFPPVAEP
                     LHAGFQRDSL"
BASE COUNT          359 a          703 c          644 g          332 t
ORIGIN      
        1 tcaccgccta ctcgtcgatc gagggcgcga gtgaggcgat gcgtgagggc gcgttccact
       61 acatccccaa gcccgtccag aaccaggaag ttctcctgac cgtgcgaaag ggctccgagg
      121 cgaggcggtt gaccgaagag aaccgccgcc tgaaagagga actctcgaag cgatacggcc
      181 tcggccgcat cgtcggcaag tccgagacga tgcggaaggt cttcgacctc gtccggctcg
      241 cgggtccttc caagtccacg atcctggtcg agggagagag cggcacgggc aaggagctcg
      301 tcgcccgggc gatccacacg cactcgccgc gctccggcgc gccgttcgtg acggtgaact
      361 cgggctcgat gccccccgac ctcctcgagt cgaacctctt cggccacgtt cgcggcgcct
      421 tcacgggagc cgtcgcgaac aaaaagggtc ttttcgaggt cgcggagggg ggctcgatct
      481 tcctggacga gatcgggacg gtgggattgg agacgcaggc gaagctcctg cgcgtgatcc
      541 aggaaaagga gttcatgcgc ctgggctccg tcgaaacgca gaaggccgac gttcgcatca
      601 tcgccgcgac caacgtcgac ctgaagaagc tcgtggtcga ggggcgattc cgggaggatc
      661 tgtactaccg cctctgcgtg atcaccatcc ccatcccgcc gcttcgcgag cggcgagaag
      721 acattccgct cctcgcggat cactttgtcc ggctctatgc gtccgagaac aacaagccga
      781 tctcgggcct cgtgcccgag tccatgaaag ctctcctcga ttacgactgg cccggcaacg
      841 tgcgcgagct cgagaacgcg atcgagcgcg cggtcgtgct ctgttccgcc ggatccatcg
      901 gcctcgatct gctgcccgaa acggtgctgc agcccggccg gggcgaagtc atggtgcggc
      961 tgccggagaa cggctcgacc tatcgcgacc tgatcgagga gtacgagcgc cgcctgatcc
     1021 gcaccgcgct tcgccgcacc ggcggcgttc agaagcgcgc cgccgagctc ctgaagttga
     1081 aaccgacgac cctgcacgag atcatcaagc gcctcgagat ccgcgaccag taagtctcgg
     1141 ccgccggctc gagaatctcc ctgccaaagg agcatcggtt gagaaaagtc gcgttcgccc
     1201 tcctccttct ggtggccctc gttccggcgg caatcgccga cgacgatccc ctgttttccg
     1261 atcgcgggat tccctcgaac tacgcgccct cacagatgga cgtgcgcgtt ccgactcccg
     1321 agaaggttct cgggttcaag atcggtgatc gattcgccac tcacgcggag gtcacggcgt
     1381 acgtgcgagc ggtcgccgcc gcggcgcccg agcgcgtgag ggtcgagacc tacggccgca
     1441 cgcccgaagg gcgcgagctg ctcgtggtcg tcgtgacgtc gcccgagaac catgcccggt
     1501 tcgacgagct cgccgcgaaa atccgcgcgc tctactcccc ttcttccgcg aagcctgccg
     1561 cgatcccggc ggacctgccg gtcttcgtct ggttctctta cggagtgcat ggagacgaag
     1621 cctcgagctc cgacgccgcc gtggcgacgc tctaccacct cgccgcctcg cgcgacgcca
     1681 acacggcaga gctgctgcgc cgggtcgtgt tcaccatgga cccgatggtg aatcccgacg
     1741 ggcggatgcg gtacctgtcg tggttcacga cggcagtcaa cggcgaaccc gacccgaatc
     1801 ccgacgcccg cgagcaccat cccagctggc cccgaggacg cgggaatcac ttcgggttcg
     1861 acctgaaccg cgactgggca tggctcactc agccggagac ccgcgcgcga atcgagcgat
     1921 accggaagac cccgccgcag gtgcacgtcg actttcacga gatgagcccg gagagctcgt
     1981 acttctttcc gccggtggcc gagccgctcc acgcgggctt ccagcgcgac tcgctcaa
//
LOCUS       DASWAV010000443         4413 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U2665
            k141_546603, whole genome shotgun sequence.
ACCESSION   DASWAV010000443 DASWAV010000000
VERSION     DASWAV010000443.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960316
            Sequence Read Archive: SRR10260007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 4413)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4413)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:47:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,929
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4413
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_546603"
                     /isolate="SMAG_U2665"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.36444 N 132.31889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..293)
                     /locus_tag="VF958_09185"
     CDS             complement(<1..293)
                     /locus_tag="VF958_09185"
                     /inference="COORDINATES: protein motif:HMM:NF012349.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HEX9149319.1"
                     /translation="MNLFVANWKMNMTRAQARAYARELGPRVGEGIAGAELVIAPPFT
                     ALDAARDDRGRWSIAAQNVSEHGAGAFTGEVSSAMLADAGCRYVIVGHSERRTL"
     gene            complement(334..1503)
                     /locus_tag="VF958_09190"
     CDS             complement(334..1503)
                     /locus_tag="VF958_09190"
                     /EC_number="2.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011391807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004618 - phosphoglycerate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HEX9149320.1"
                     /translation="MKSIRDLPLDQKRLFLRVDFNVPLSDGKVLDATRIVETLPTIRH
                     AIERGARIVCASHLGKPKGKPKPDLSLAPVAVELARHLQRPVRFADDCIGPQVGKLVS
                     ELAPGEALLLENLRFHPGEEANDPEFARALAALADVYVNDAFGSSHRAHASVTGVPAL
                     LEAKGAGFLLEKEVRELSRLLDPERPFAAILGGAKISGKIDTLRALARRADTLLVGGG
                     IANHFVRAIGLSVGLSLLEEDKVSVAREVLDFCRANRKELALPTDFVVAASPDDGAHA
                     RTVGISKIPPDAMALDVGQKTLEQFERLLEPARTIFWNGPLGVFEKPPFDKGTRALTL
                     LLAESDAVTVVGGGESVAAVQQAGLADRFTHVSTGGGASLEFLANGRLPGIDALS"
     gene            1588..3294
                     /gene="ggt"
                     /locus_tag="VF958_09195"
     CDS             1588..3294
                     /gene="ggt"
                     /locus_tag="VF958_09195"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HEX9149321.1"
                     /translation="MRRFAAALAILLLLLPGVSFATAPQVARGTGGAVAAAEENAARA
                     GIEILRRGGNAADAAVAVAFALAVTWPEAGNIGGGGFWISRDANGRMLAIDFRETAPR
                     NARRDLFIRPGRGGQPPSSTEGPLASGVPGSVDGLWRAHRSAGRLSWKTVIEPAVRLA
                     REGFVMTETVSESIAAPRYRARLAVDPETAAIFLPHGQPPVPGTVFRQPALARTLEAI
                     RDRGSDGFYRGQVAREIEEGQKKAGGLITRGDLALYSARVRPALRFRFGPAEIVTTPA
                     PSSGPVLAEMAILASSLGLEKLKGRDPAASHLLVEIEKRAFHDRNRWLGDPAFPGVRE
                     GAFTDSARLRRLAATINPNRATPSEALQRSREEPPSTTHFSVVDAAGGAVSVTTTLND
                     SFGNARVAPGLGFLLNNEMDDFATAPGKPNLYGLIQGEVNAVAPGKRMLSAMCPSIAV
                     IGKRNAFVWGTPGGSTIPTTNFQILLGLILRGESLEAAVAAPRFHQQDFPDKIQLERE
                     RFDSEWTDALRRIGHTVEEREPDKDPIGRVHAIARKPDGSLTAVADPRSGGVGLVVSE
                     RR"
     gene            3300..4370
                     /gene="mtnA"
                     /locus_tag="VF958_09200"
     CDS             3300..4370
                     /gene="mtnA"
                     /locus_tag="VF958_09200"
                     /EC_number="5.3.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012099157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046523 -
                     S-methyl-5-thioribose-1-phosphate isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0019509 - L-methionine salvage from
                     methylthioadenosine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-methyl-5-thioribose-1-phosphate isomerase"
                     /protein_id="HEX9149322.1"
                     /translation="MLRPVYREGRLLRVLDQRLLPSQEVWLTMEKTPEVAIAIREMVV
                     RGAPTIGVAAAYGAAFAMRSGAATLPSDRFETARRVLAATRPTAVNLFASLDRMERRF
                     REVAGDPPERIETALVEEADRVAAETLESSRRLGRYGAEILSTGMTVLTHCNAGGLAT
                     GGYGSALGVIRAAVEEGKAVRVLAPETRPFLQGSRLTAWELARDGIPVEVITDSMSGH
                     YLSRGEIAAVVVGADRIAANGDTANKIGTYSLAVLAKENGVPFYVAAPTTTIDLACPD
                     GTAIPIEERAAAEVLSFAGHSIAPAGVGARHPAFDVTPARYIRAIVTDEGICRPPYAD
                     SLAAAVRAATDRRSRQSVVGNR"
BASE COUNT          685 a         1536 c         1522 g          670 t
ORIGIN      
        1 agcgtcctcc tctcggagtg cccgacgatg acgtaacggc aacccgcgtc cgcgagcatt
       61 gcggaagaga cctcgccggt gaaggctccc gcgccgtgct ccgagacgtt ctgcgccgcg
      121 atcgaccagc gcccgcggtc gtcgcgcgcg gcgtcgagcg ccgtaaacgg cggcgcaatg
      181 acgagctcgg ccccggcgat cccctccccg actcgcgggc cgagctcgcg tgcatacgcg
      241 cgcgcctgcg ctcgcgtcat gttcatcttc cagttggcga cgaagaggtt catggagtcc
      301 gggatcggaa atcggaaatc ggagatcgag gggtcacgag agcgcgtcga ttccgggaag
      361 ccggccattg gcgaggaact cgagcgatgc gccgcctccc gtcgagacgt gcgtgaagcg
      421 gtcggcgagg cctgcctgct ggaccgccgc gacgctctcc ccccctccca cgaccgtgac
      481 cgcgtcggat tccgcgagaa ggagcgtgag cgcccgggtt cccttgtcga acggcggctt
      541 ctcgaagacg ccgagcgggc cgttccagaa gatcgtgcga gccggctcga gcagccgctc
      601 gaactgctcg agggtcttct gtcccacgtc gagcgccatc gcgtcgggcg gtatcttcga
      661 gatgcccacc gtacgcgcgt gcgcgccgtc gtcgggcgag gcggcgacga cgaagtccgt
      721 gggaagcgcg agctcctttc ggttcgcgcg acagaagtcg aggacctctc gcgccacgga
      781 gaccttgtct tcctcgagca gcgaaaggcc gacggaaagt ccgatcgccc gcacgaagtg
      841 attcgcgatg ccgccgccga cgaggagcgt gtccgcgcgg cgcgcgagcg cgcgcagagt
      901 gtcgatcttc ccggagatct tggcgcctcc gagaatcgcc gcgaacggcc gctcggggtc
      961 gagcaatctc gagagctcac gcacctcttt ttccaggagg aagcccgctc ccttcgcctc
     1021 caggagggcc gggacgcccg tcaccgatgc gtgcgcacgg tgcgaggaac cgaacgcgtc
     1081 gttgacgtag acgtccgcga gggcggcgag cgcccgcgcg aactccggat cgttggcctc
     1141 ttcgcccgga tggaatcgga ggttttccag tagcaatgct tctcccgggg cgagctcgga
     1201 aacgagcttc ccgacttgcg gtccgatgca gtcgtccgcg aagcggaccg ggcgctgcaa
     1261 gtgccgcgcg agctcgacgg cgaccggggc gagcgacagg tccggcttcg gctttccctt
     1321 cggcttcccg aggtgagagg cgcacacgat ccgcgccccc cgttcgatcg cgtggcggat
     1381 cgtcggcagt gtctcgacga tgcgggtcgc gtcgagcacc ttgccgtcgg agagcggtac
     1441 gttgaagtcg acgcgcagga aaagcctctt ctggtcgagc gggagatctc ggatcgactt
     1501 catgagatgg gatcgtaaat cgggaaccgg caatcgggaa tcgggggaat gggattttcc
     1561 gtttccccgc ctcgatagaa tcgccggatg cgtcgttttg cggccgccct tgccatcctg
     1621 ttgctgctcc tgcccggcgt ctcctttgcc acggcgccgc aggtcgcccg cggaaccggc
     1681 ggagcggtcg cggccgcgga ggagaacgcg gcgagagcgg gcatcgagat cctccggcgg
     1741 gggggcaacg ccgccgacgc ggccgtcgcc gtcgcgttcg cgctcgccgt gacgtggccg
     1801 gaggcgggaa acatcggcgg gggcggcttc tggatctccc gcgacgcgaa cggtcgaatg
     1861 ctcgcgatcg acttccgcga aactgcgccg cgaaacgcgc gtcgggacct cttcatcaga
     1921 ccgggacgag gcgggcagcc cccctcctcg accgaggggc ccctggcgtc gggggtcccc
     1981 gggtcggtcg acggcctctg gcgcgcccac cgatcggcgg ggcggctctc ctggaaaacc
     2041 gtgatcgaac cggcggtgcg gctcgcgcgt gagggattcg tgatgaccga gaccgtctcg
     2101 gaatcgatcg cggcgccgag atatcgtgcg cggctggccg tcgaccccga gacggcggcg
     2161 atttttctcc cccacggaca acctcccgtc cccggaactg tcttccggca gcccgcgctc
     2221 gcccggacgc tcgaagcgat ccgggatcgc ggctcggacg ggttctaccg cggccaagtc
     2281 gcgcgcgaaa tcgaggaggg gcagaagaag gcgggcggtc tgatcacccg cggcgacctc
     2341 gcgctctaca gcgcccgggt gcggccggcg ctccgcttcc gattcggtcc tgcggagatc
     2401 gtgactacgc cggcgccgtc ctccggtccc gttctcgccg agatggcgat cctcgcgtcg
     2461 tccctcggcc tcgagaagct caaagggcgc gacccggcgg cgtctcatct cctggtggag
     2521 atcgagaaga gagccttcca cgaccgcaac cgctggctcg gcgatccggc gtttccgggg
     2581 gtccgagagg gagctttcac cgactccgcg cggctgcgcc gactcgcggc gacgatcaat
     2641 cccaatcgcg cgacgccctc ggaagcactc cagcgctcgc gggaggagcc gccctcgacg
     2701 acccacttct ccgtcgtcga cgcggcggga ggcgccgtct cggtcacaac gaccttgaac
     2761 gattccttcg ggaacgcacg ggtcgctccg ggactcggct ttctcctcaa caacgagatg
     2821 gacgacttcg ccaccgcgcc cggcaagccg aacctctacg gcctgatcca gggcgaggtc
     2881 aacgcggtcg cccccgggaa gcggatgctc tccgcgatgt gtccctcgat cgcggtgatc
     2941 ggaaagcgaa acgcgttcgt gtgggggact cccggcggct ccacgattcc cacgacgaac
     3001 ttccagatcc tgctcggcct gatcctgcgc ggggagagcc tcgaggccgc cgtcgccgca
     3061 ccccggttcc accagcagga tttccccgac aagattcagc tcgagcggga gcgattcgac
     3121 tcggaatgga cggatgcgct gcgccggatc gggcacaccg tcgaggagcg cgagcccgac
     3181 aaggacccga tcggccgcgt tcacgcgatc gcgcggaagc ccgacggaag cctgactgcc
     3241 gtcgcggatc ctcgaagcgg cggcgtcggg cttgtcgtct cggagcgccg ttgacggaaa
     3301 tcctccgccc ggtctatcgg gaaggccgtc tcctccgggt cctcgaccag cgcctgctcc
     3361 cctcgcagga ggtctggctc acgatggaga agaccccgga ggtcgcgatc gcgatccgcg
     3421 agatggtggt tcgcggcgcc cctacgatcg gagtcgcggc ggcgtacggc gcggctttcg
     3481 ccatgcggag cggcgcggcg actctgccct cggaccgctt cgagaccgca cgacgcgtgc
     3541 tcgcggcgac gcggccgacc gctgtgaacc tgttcgcgtc gctcgaccgc atggagcggc
     3601 gtttccgcga agtcgccgga gatccgcccg aacggatcga gaccgccctc gtcgaagagg
     3661 cggaccgggt cgccgccgaa acgctcgagt cgtcgcgccg gctcggacga tacggggccg
     3721 agatcctctc caccggaatg accgtgctga cacattgcaa cgccggaggg ctcgcaaccg
     3781 gcggctacgg cagcgcgctc ggcgtcattc gcgccgcggt cgaagagggc aaggccgtcc
     3841 gcgtcctcgc gccggagacg cgccccttcc tccagggctc gcggctcacg gcatgggagc
     3901 tcgcgcgcga cggaattccc gtcgaagtca tcacggacag catgagcggg cattacctct
     3961 cgcgcggcga gatcgccgcg gtggtcgtcg gcgccgaccg gatcgcggcc aacggcgaca
     4021 cggcgaacaa gatcggaacg tattcgctcg ccgtgctcgc gaaggagaac ggcgtcccgt
     4081 tctacgtcgc ggcgcccacg acgacgatcg acctcgcctg ccccgacggc acggcgatcc
     4141 cgatcgagga gcgcgcggcc gccgaggtcc tgtcgttcgc cgggcactcg atcgcgccgg
     4201 ccggagtggg ggcgaggcac cccgcgttcg acgtcacgcc cgcccgatac atccgtgcga
     4261 tcgtgacgga cgaggggatc tgccgccccc cctacgccga ctcgcttgcc gctgccgttc
     4321 gcgccgcaac cgatcgtcgg agccggcaat cggtcgtcgg caaccggtaa ccgagtccga
     4381 tttcgtcatg gctataatgg cgtcatgaag aac
//
LOCUS       DASWAV010000444         5640 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U2665
            k141_63656, whole genome shotgun sequence.
ACCESSION   DASWAV010000444 DASWAV010000000
VERSION     DASWAV010000444.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960316
            Sequence Read Archive: SRR10260007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 5640)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5640)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:47:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,929
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5640
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_63656"
                     /isolate="SMAG_U2665"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.36444 N 132.31889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..995
                     /locus_tag="VF958_09205"
     CDS             <1..995
                     /locus_tag="VF958_09205"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX9149323.1"
                     /translation="FRAMRAEIAGMSRPLQPPGASDPETVKKLASLGYIGAAAPSAAN
                     EDLPDPKDRIGTIDQLKQASRLAAEFRDAEGIALLSRLAEQNPRMLDVWETLARLLRR
                     AGRTREALAALERADRLAPGTPQVMLGLADLHLENRDFDRARALALAAGEAGAPGFRE
                     ELAAIAFAAGDLESARREAEAVLAKTPGARAPRLVLARIALARGDLAGALALLDQAAE
                     IDRASARTSAVGLRSLRGDVLARLGREKEAEADFRLEVASHPENLDGWARLALLYASQ
                     GRGADLDGLLSEMTKTIPTPQGYDAALRVSRIIGDSARTRDWKRRKIARFGAGG"
     gene            1242..4193
                     /locus_tag="VF958_09210"
     CDS             1242..4193
                     /locus_tag="VF958_09210"
                     /inference="COORDINATES: protein motif:HMM:NF019335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEX9149324.1"
                     /translation="MPKRFHFHRWAIALLAIAVLAAPALAQYGQSTGGIQGRVTDEQG
                     GVLPGVQVTLRGPGAPQTAYTDARGEFRVANLDAGVYTLTLTLAGFQTVNRENVTVNI
                     GRNTELTIPMGLTRVEATVTVSSEAPLLETKRVQTSAVVSQQELKSIPSARDPWVVLQ
                     SAPGVQIDRMNVAGSESGQQSNFSAHGSPAGTFTVDGVNVTDMSALGSSAAYYDFDMF
                     QEMQVITGGGDASISGSGAHLNMVTKRGTNDLHGSARVFGVDERFESQNLPEEAKTQV
                     GALGSGNHIQSLQDYGAEAGGPIWRDHLWLWGSYGRDQINLVTAGGASDKTTLENLNA
                     KLNWQIIPSNAANGWWMHSDKLKFGRNAGPEHPQETTWDQTLPQNTWKIEDSQVFSSS
                     LFATVQYNGINGNFTLDPEGGNRQTFKDEGGVWHNSYEIYAAPRPQRQVKGDVSYFFN
                     TGNLGHELKAGFGYLRTGARSSSSWPGSAGTGANGLAGETYGDLFDCTDDDGNSIPCA
                     VITRRSNLVVDNKYWSGYLQDTMSFDRLSVNLGVRYDYQTGENAPTVVAANPSFPQIL
                     PAIDYPGSGQPFTWKDWQPRVGLTYSLGANRSTVLKASYARYAEALGTGLVDNVNPLS
                     AVSYAYYGWNDLNHNNLVEVGEVNLSNFISSSNYDPANPGAAVSVHSFGSGIHAPNTD
                     EFLAGIDHELFPAFAVGVAYTYRKFNDLLFRSRTGLTSADYVQYTTITGTLPDGTAYS
                     APVYRIRDGIDTPPGYVYDNRPGYDQTYNGVDFTLTKRLANRWMARGSFTYNWNKQHA
                     GSGACVDPTNVVPGQGSANAGDPQTAYTAQTCADNVVAGFRSTGSGDKSSVFLSSKWQ
                     FYVNAMYQLPLGFNVAGSFFGRQGYPINWWRLVTGPDGERRDVAVSGLDDQRYDNVFE
                     LDGRIEKVVPITTTANLTISADVFNITNEDTILQRFNRLNRSGLAVGNTGEIKEIQSP
                     RVWRFGLRLAF"
     gene            4559..>5640
                     /locus_tag="VF958_09215"
     CDS             4559..>5640
                     /locus_tag="VF958_09215"
                     /inference="COORDINATES: protein motif:HMM:NF019335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEX9149325.1"
                     /translation="MPKRFHFHRWAIALLAIALAVPAFGQYGQNTGGIQGRVTDEQGG
                     VLPGVQVTLRGPAAPQTVYTDARGEFRVANLDPGVYTLTLTLAGFQTVSRENVTVNLG
                     RNTELTIPMGLTRVEAAVTVSSEAPLLETKRVLTSTVVSQQELKSIPSARDPWVVLQS
                     APGVNIDRMNIAGSESGQQSNFSAHGTGGGTFTVDGVNVTDMSALGSSAAYYDFDMFQ
                     EMQVITGGGDASIAGSGAHLNMITKRGTNDVHGSARVFGVDERFESQNLPEEARTQAG
                     ALTSGNHIQSLQDYGAEAGGPVWKDHLWLWGAYGRDQINLITAGGAFDKTTLENLNAK
                     LNWQIIPSNAANGWWMHSDKIKFGRNA"
BASE COUNT         1039 a         1888 c         1785 g          928 t
ORIGIN      
        1 cgttccgcgc gatgcgcgcc gagatcgccg gcatgagccg gccgctgcag ccgccgggtg
       61 cctcggaccc cgagacggtc aagaagctcg cgtcgctcgg gtacatcggc gccgccgcac
      121 cgagcgcggc gaacgaagac ctgcccgacc cgaaagaccg gatcggcacg atcgaccagc
      181 tgaagcaggc ctcgcgtctt gcggcagagt tccgcgatgc ggagggaatt gcgctgctgt
      241 cgcgtctcgc ggaacagaac ccgcggatgc tcgacgtctg ggagactctc gcgcgtcttc
      301 tccggcgagc cggtcggacc cgcgaagcgc tcgccgcgct cgagcgggcg gatcgtctcg
      361 cccccggcac gccgcaagtc atgctcggcc tggcggacct gcacctggag aatcgcgatt
      421 tcgaccgcgc acgggcgctc gcgctggctg cgggagaggc cggagccccc ggatttcgcg
      481 aagagctcgc cgcgatcgcg ttcgccgccg gggacctcga gtccgcgcgt cgggaagccg
      541 aagcagtcct cgcgaagacg ccgggagcac gcgcgccgcg cctcgtgctc gcacgcatcg
      601 cccttgcccg cggcgacctc gccggggcgc tcgcgctcct cgaccaggcg gccgagatcg
      661 atcgcgcctc cgcgcgaacg tccgccgtag gacttcgctc gctccgcggg gacgtgctgg
      721 cgaggctcgg ccgggagaag gaggcggagg ccgacttccg cctggaagtt gcgagccacc
      781 ccgagaatct cgacggatgg gcgcggctcg cacttctcta cgcgagccag ggccgaggcg
      841 ccgatctcga cggcctcctc tcggagatga cgaagacgat cccgacgccg caaggctacg
      901 acgcagctct gcgcgtttcc cggatcatcg gagactccgc gcgcactcgc gattggaaga
      961 ggcgcaagat cgcccgcttc ggcgccggag gttgatgctc tcgcggggcg gtcctccgcc
     1021 ctgatttttt ggagagaaat ccaaaaagac gttcgtttcg ccttcgcgtc gtgcgcgaac
     1081 gagcgcgcgg aaacacccct tcatccggag cgctgatcct ctcgtcgtcc gagtcgtccg
     1141 gctcgtccct ctttcgctct tcgcgggccg tggaattgct caatgggcat gcgtccgtgc
     1201 cagcactgcg cacgaacttg cacgaaacga aaggagatcg catgccgaaa cggttccact
     1261 ttcatcgatg ggcgatcgcg ctcctggcaa ttgccgtcct tgccgcaccg gctctcgcgc
     1321 agtacggcca gagcaccggc gggatccagg ggcgcgtcac ggacgagcag gggggcgtcc
     1381 ttcccggcgt ccaggtgacg ctccgcggtc ccggcgcgcc gcagacggcg tacaccgacg
     1441 cgcgcggcga gttccgggtc gccaacctcg acgcgggcgt ctacaccctg acgctgactc
     1501 tcgcgggatt ccagaccgtc aaccgggaga acgtcacggt caacatcggg cgcaacaccg
     1561 agctgacgat cccgatgggt ttgacgcgcg tcgaagcgac ggtcaccgtc tcgagcgagg
     1621 cgccgctgct ggagaccaag cgcgtccaga cctcggccgt cgtcagccag caggagttga
     1681 agtcgattcc gagcgcgcgc gacccctggg tcgtcctcca gagcgcgccg ggagttcaaa
     1741 tcgaccggat gaacgttgcg ggcagcgaga gcggccagca gtcgaacttc tccgcccacg
     1801 gttcaccggc gggaacgttc acggtggacg gcgtcaacgt gaccgacatg tcggctctcg
     1861 gttcgtcggc ggcctactac gacttcgaca tgttccagga gatgcaggtc atcacgggag
     1921 gcggcgacgc gtcgatctcc gggtccggtg cgcacctgaa catggtgacg aagcgcggga
     1981 ccaacgacct ccacgggagc gcccgtgtgt tcggcgtcga cgagcggttc gagtcacaga
     2041 accttcccga agaggcgaag acccaggtgg gcgcgctcgg gtcgggcaat cacatccaga
     2101 gcctccagga ctacggcgca gaagccggag gaccgatctg gagagatcac ctctggctgt
     2161 ggggctccta cggccgggat cagatcaacc tggtcaccgc gggcggcgcg tcggacaaga
     2221 cgacgctcga gaatctgaac gccaaactca actggcagat catcccgtcc aacgcagcca
     2281 acggctggtg gatgcacagc gacaagctga agttcggtcg caatgcggga ccggaacacc
     2341 cccaggagac cacctgggac cagacccttc ctcagaacac gtggaagatc gaggactcgc
     2401 aggtcttctc gtcgagtctc ttcgccaccg tccagtacaa cgggatcaac gggaacttca
     2461 cgctcgatcc ggaaggcggc aaccgccaga ctttcaagga cgaggggggc gtctggcaca
     2521 actcctacga gatctatgcc gcgccgcgtc cgcagcgtca ggtcaagggg gacgtctcgt
     2581 acttcttcaa caccgggaac ctcggccacg agctgaaggc gggattcggg tacctgagga
     2641 ctggcgcccg ctcgagctcg agctggccgg gttcggccgg cacaggcgcc aacgggctcg
     2701 ccggcgaaac ctacggtgat ctcttcgatt gcaccgacga cgacggcaat tcgattccct
     2761 gcgccgtcat cacccggcgg tcgaacctcg tcgtcgacaa caagtactgg agcggatacc
     2821 tgcaggacac gatgtccttc gatcgactct ccgtcaacct cggtgtccgc tacgactacc
     2881 agacgggcga gaacgcgccg acggtcgtcg ccgcaaaccc ctcgtttccg cagatcctgc
     2941 cggcgatcga ctacccggga tccgggcagc cgttcacctg gaaagactgg cagccgcgcg
     3001 tcggtctcac ctactcgctc ggagccaacc ggagcacggt gttgaaagcg agctacgccc
     3061 gctatgccga ggctctcggc acggggctcg tcgacaacgt gaacccgctt tcggccgttt
     3121 cgtacgcgta ctacggctgg aacgacctga accacaacaa cctcgtcgag gtcggcgagg
     3181 tcaacctctc gaacttcatt tcatccagca actacgatcc cgccaacccg ggagcagccg
     3241 tttcggtcca ctcgttcggg tcggggatcc atgcgcccaa caccgacgag ttcctggccg
     3301 gaatcgacca cgagctcttc ccggcgttcg ctgtcggggt tgcctacacc tatcgcaagt
     3361 tcaacgatct gctcttccgc tctcggaccg gactgacgag cgcggactac gtccagtaca
     3421 ccacgatcac cggcacgctc ccggatggca cggcctacag tgcgccggtc taccggatcc
     3481 gggacggaat cgacacgccg cccgggtacg tctacgacaa ccggccgggc tacgatcaga
     3541 cctacaacgg cgtcgacttc acattgacga agcggctcgc caaccgctgg atggctcgcg
     3601 ggagcttcac ctacaactgg aacaaacagc acgctggctc cggcgcctgc gtggatccga
     3661 ccaacgtcgt tccgggacag ggttccgcca atgccgggga tcctcagacc gcctacacgg
     3721 cgcagacctg cgccgacaac gtcgtcgccg ggttccgttc gacgggcagc ggcgacaagt
     3781 cgagcgtctt cctctcgtcg aagtggcagt tctacgtgaa cgccatgtac cagcttccgc
     3841 tcgggttcaa cgtcgccggc agcttcttcg gacggcaggg ctatccgatc aactggtggc
     3901 gtctcgtgac gggtcccgac ggagagcggc gtgacgttgc ggtgtcgggt ctcgatgacc
     3961 agaggtacga caacgtcttc gagctcgatg gtcgcatcga gaaggtcgtc ccgatcacca
     4021 ccaccgcgaa cctcacgatc tccgcggacg tcttcaacat cacgaacgaa gacacgatcc
     4081 tgcagcggtt caatcgcttg aaccggtcgg gtctcgccgt gggaaacacg ggcgagatca
     4141 aggagattca gagccctcgg gtgtggcgat tcggcctccg gctcgcgttc tagcttcctt
     4201 tcgacttctc ttcagccgcc cgggaaaccg attcccgggc ggctttttct tggctccagg
     4261 tcagacaatc cggtcgagcc attccccgcc tttcgaggtt cttccagccg ggcggacggc
     4321 cgcagtcgtc cgcccgtcct ccaaatcctt ggactgggat tcaaaagctg agatttcggg
     4381 gctggagttc agaactcccc caaccatata attcgcagat tggaagtgag ttacaactga
     4441 ctgatccctc tggatcgggg cctgatcccg tggcgctcga attgctttgc accgtgcaga
     4501 gcttgctgtg tcaattgcac tacctcacgt gggcttccca gtgacgaaag gaggattcat
     4561 gccgaaaagg tttcatttcc atcgatgggc catcgcgctc ctggcgatcg ccctcgccgt
     4621 accggcgttt ggtcagtacg gtcagaacac gggcggaatt caggggcgcg tcacggacga
     4681 gcagggaggc gtcctgcccg gcgtccaggt cactctccgt ggccccgccg cgccgcagac
     4741 cgtctacacc gatgcccgcg gcgagttccg ggtcgccaac ctcgatcccg gtgtctatac
     4801 gctgacgctg accctggcgg gtttccagac cgtcagccgg gagaacgtca ccgtcaacct
     4861 cgggcgcaat acggagctga cgatcccgat gggcctgacg cgggtcgagg ccgcggttac
     4921 ggtttcgagc gaggctccgc tgctggagac caagagggtc ctgacctcga ccgtcgtcag
     4981 ccagcaggag ttgaaatcga tcccgagcgc gcgtgatccc tgggttgtcc tccagagcgc
     5041 gccgggcgtg aacatcgacc gcatgaacat cgccggcagc gagagcggcc agcagtcgaa
     5101 cttctcggcc cacggaaccg gcggtggcac gttcacggtg gacggcgtca acgtgacgga
     5161 catgtcggct ctcggttcgt ctgcggccta ctacgatttc gacatgttcc aggagatgca
     5221 ggtcatcacc ggcggcggtg acgcttcgat cgccggctca ggcgcccatc tcaacatgat
     5281 cacgaagcgc ggcacgaacg atgtccacgg cagtgctcga gttttcggag tggacgagcg
     5341 cttcgagtcg cagaacctgc cggaggaagc caggacccag gcgggggcgc tcacttccgg
     5401 caaccacatt cagagccttc aggactacgg cgcggaagcc ggaggaccgg tgtggaaaga
     5461 ccacctctgg ctgtggggcg cgtacggccg cgaccagatc aacctgatca ccgcgggagg
     5521 cgcgttcgac aagacgacac tcgagaacct gaacgccaaa ctcaactggc agatcattcc
     5581 ctccaacgcg gcgaacggct ggtggatgca cagcgacaag atcaagttcg gccgcaacgc
//
LOCUS       DASWAV010000445         2034 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U2665
            k141_382501, whole genome shotgun sequence.
ACCESSION   DASWAV010000445 DASWAV010000000
VERSION     DASWAV010000445.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960316
            Sequence Read Archive: SRR10260007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2034)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2034)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:47:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,929
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2034
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_382501"
                     /isolate="SMAG_U2665"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.36444 N 132.31889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            50..721
                     /locus_tag="VF958_09220"
     CDS             50..721
                     /locus_tag="VF958_09220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015152422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyltransferase family protein"
                     /protein_id="HEX9149326.1"
                     /translation="MGYLFRNRREAGRALGTELGAYTGRDDVVILGLPRGGVPVAFEV
                     AAMLEAPLDVFLVRKLGAPGQEELAMGAIASGGVRILNPAVIESLRVSADEIERVSKR
                     EEVELRRRELSYRGERPPLDLHGRIAILVDDGLATGSSMHAAVEAARKFGPAQVVVAV
                     PVAPPSTAREMTRIADDVVCVATPEPFYSVGRFYEEFDQTTDDEVRDLLGRAAPVAVE
                     EGQTR"
     gene            718..1053
                     /locus_tag="VF958_09225"
     CDS             718..1053
                     /locus_tag="VF958_09225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9149327.1"
                     /translation="MNVQRVPIAELPSFLERFSRGHHGWLTHVEVEDETHRGEIDEGA
                     QPLGGIVADRDRNRVLVFLDRSPRGSIRYGIDQPTGLWRETSEGGVRLELDSADGSTT
                     TLECHPAAH"
     gene            1332..1799
                     /locus_tag="VF958_09230"
     CDS             1332..1799
                     /locus_tag="VF958_09230"
                     /inference="COORDINATES: protein motif:HMM:NF012780.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEX9149328.1"
                     /translation="MRLRDVMRQEVETISPRESAAAAFERMRRAKIRHLVVKDGRKVV
                     GVLSDRDVVGLGPLRQVETVEEVMTAPAVCGSPDLTIREAANLMRGRTMGCLPVLEEG
                     KVVGIVTVTDLLQLVGAGIERPVPKTRRWVLKDRGPRRRPAGRGRTIRAPAAR"
     gene            1844..>2034
                     /locus_tag="VF958_09235"
     CDS             1844..>2034
                     /locus_tag="VF958_09235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011300591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HEX9149329.1"
                     /translation="MKKIWMVAPLGAGIGAALMYLFDPERGKRRRALVRDKLVHATHA
                     TAQKVGVASRDATNHLQGL"
BASE COUNT          350 a          660 c          690 g          334 t
ORIGIN      
        1 gcaaaacaca tcatgcacgg acgccgtttc cgcaccacaa tttgtcgcgg tgggttacct
       61 cttccggaat cgccgcgaag cgggtcgcgc actcggcacc gagctcggcg cctacaccgg
      121 ccgcgatgac gtcgtcattc tcggtctgcc gaggggcggc gtgccggtcg cgttcgaagt
      181 cgcagcgatg ctcgaggcgc ctctcgacgt gttcctcgtc cgcaagctcg gagcgcccgg
      241 ccaggaggag ctcgcgatgg gcgcgatcgc atcgggagga gttcgaatac tcaatcccgc
      301 ggtgatcgag tcgctgcgcg tctccgccga cgagatcgaa cgcgtttcga agcgggaaga
      361 ggtcgagctt cggcggcgcg agctttcgta ccgcggagag cgaccgccac tcgatctcca
      421 cgggcgaatt gccatcctgg tcgacgacgg tctggcgacg ggctcttcga tgcacgctgc
      481 ggtcgaagcg gcgcggaagt tcgggccggc gcaagtcgtc gttgcggtac cggtggcgcc
      541 gccgtcgacc gcccgcgaaa tgacgagaat cgcggacgac gtcgtctgcg ttgccactcc
      601 cgaacccttt tattcggtgg gccgtttcta cgaggagttc gaccagacca cggacgacga
      661 ggtccgcgac ctccttggca gagcagcccc ggttgccgta gaggagggac agacgcgatg
      721 aacgtccaac gtgtaccgat cgccgagttg ccgagctttc tcgaaaggtt ttcgcgggga
      781 caccacgggt ggctcaccca cgtcgaggtg gaggacgaga cccatcgcgg cgagatcgat
      841 gagggcgcgc aacccctggg cggcatcgtc gcggaccggg accgcaatcg ggtgctcgtg
      901 ttcctcgaca ggagtccgcg gggatccatc cgctacggga tcgatcagcc gacgggactc
      961 tggcgggaga cctccgaggg aggagtgcgc ctcgagctgg actccgccga cggttcgacc
     1021 acgaccctcg agtgccatcc ggctgcacac tgaagtttgt ttcccgacgg ctgaagtccc
     1081 gcgccgcgga ttggtgagtt caaatcatgc ggccccgcgt tcgccgcgct aaccttcact
     1141 tgttcgtctc gaggttcgct ctaccagaac agccgaagga cggaccgggg aggcgcgagc
     1201 cggacggcgt ggcgtaacgc agccggtcat cgcgcctccg cttttctgct cttccccgct
     1261 ttcagctggt ctcttcacat caggcggccg cggcgcggag gcgctagcct gactcacgcg
     1321 aggaggtaca tatgaggctg cgagacgtca tgcgccagga agtggaaacg atctccccgc
     1381 gagaatccgc cgcggcggct tttgaacgga tgcgccgcgc gaaaatccga cacctcgtcg
     1441 tcaaggacgg ccgaaaagtc gtcggcgtcc tgtcggaccg cgacgttgtc ggcctcggcc
     1501 cgttgcgcca ggtcgaaacg gtcgaggagg tgatgacggc cccggccgtg tgcggctcgc
     1561 ccgatctcac gattcgcgag gccgcaaact tgatgcgcgg ccggacgatg ggttgcctgc
     1621 ccgtcctcga ggagggaaag gtcgtcggga tcgtgacggt caccgacctg ctccagctcg
     1681 tcggcgccgg gatcgaacgg ccggttccga agacacggcg ctgggttctg aaggaccgcg
     1741 ggccgcgccg tcgtccggcg ggacgcggac gcaccatccg ggcgccggcg gcgcgctgag
     1801 tgtcgcccgt cattcatcta tcgggagcga aacggaggag caaatgaaga aaatatggat
     1861 ggtcgccccc ctgggcgcgg gaatcggagc cgcactgatg tacctgttcg accccgaacg
     1921 tggaaaacgt cgccgcgcgc tcgtacgcga caagctcgtc cacgcgaccc acgcgaccgc
     1981 ccagaaggtc ggcgtcgcgt cgcgtgacgc caccaaccat ctccagggac tcat
//
LOCUS       DASWAV010000446         2862 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U2665
            k141_224852, whole genome shotgun sequence.
ACCESSION   DASWAV010000446 DASWAV010000000
VERSION     DASWAV010000446.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960316
            Sequence Read Archive: SRR10260007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2862)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2862)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:47:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,929
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2862
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_224852"
                     /isolate="SMAG_U2665"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.36444 N 132.31889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..703)
                     /locus_tag="VF958_09240"
     CDS             complement(<1..703)
                     /locus_tag="VF958_09240"
                     /inference="COORDINATES: protein motif:HMM:NF025006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX9149330.1"
                     /translation="MRFQSKIASAAVLALLFAGAGRAQDWRGRARVDGRVLNEKGEGV
                     PGAKLVFKRAGAGPEAIASDKNGRWAYLGLAGGAWDIDVEADGYLPYRTTVQLSEIER
                     IPSMVIRLQPAPKAEPTPAAGVPKNTAPDVIPILEHGNQLIEQKDFAGARAEYEKALV
                     SVPNNPIILRAIAQTYYGEKKVEMAIATLKKVVELDPNDSTALLLLADLQIEKGNLDE
                     GKATLAKVPADAIKDP"
     gene            850..1923
                     /locus_tag="VF958_09245"
     CDS             850..1923
                     /locus_tag="VF958_09245"
                     /inference="COORDINATES: protein motif:HMM:NF012766.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro peptidase family protein"
                     /protein_id="HEX9149331.1"
                     /translation="MTLDREIYRRRLESMRQRLASVAADGLYATPSSNLFYLTGIDFW
                     RSERLTALLLFPDREPVVLCPAFEEARLRGMSAVTNVVTWEETEDPFAKAAALFPAAA
                     GTLAVEPSTAFEDVERLLAHRPSWKPVSAGPAIASVRMIKTPEEIAAMRRAIAIALPR
                     FRRAFESLAPGATEAQISRAFGGDNVVQFGPSSALPHGASTERPLARDEAVLIDAWDK
                     PEGYYYDITRSTFFGRPPDEYRKIWEIVLEAQGAAIDRAAPGVPCSEVDAAARRVIEK
                     AGYGAYFTHRLGHGLGIDVHEPPYMVAHEKTLLEPGMTFTSEPGIYLPGRFGVRIEDD
                     IVCTETGAESLSPRVDRLEPIAA"
     gene            1989..>2862
                     /locus_tag="VF958_09250"
     CDS             1989..>2862
                     /locus_tag="VF958_09250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9149332.1"
                     /translation="MGEFSAAEVEEASEVGRRAAVLLLHGDRLSIENTFATEAVASKY
                     LGDDVWGRLSQRQRQVIRSIVLDRFQEALIPPRGTPAEIAWSSARPAGDTVLLFLGLR
                     YPAGVLKTRWSLARFSSEWKVLDVLLSDPGISLAAEAVRPFGRDGVRPRNSGREARAA
                     ALPRALGIVAILAVVLFLGRRLAPSSRKILVWTAAAPAVLFAVDGFFAIRRALSEPYR
                     IPEVLAPAPWSVAERQALLAQREGRLDAARRNWQAAVAAGAPAAAADYQVGLALAAAG
                     RREEAKAAFLRSLSR"
BASE COUNT          387 a         1032 c          957 g          486 t
ORIGIN      
        1 cggggtcctt gatcgcgtcg gcaggcacct ttgcgagcgt cgcctttcct tcgtcgagat
       61 tccccttctc gatctggagg tctgcgagaa gaaggagcgc ggtggagtcg ttcggatcga
      121 gctccacgac cttcttgagc gtcgcgatcg ccatttcgac cttcttctcc ccgtagtagg
      181 tctgcgcgat cgcgcgcagg atgatcggat tgttcggcac tgagacgagt gccttctcgt
      241 actccgctcg cgcgccggcg aagtccttct gttcgatgag ctggtttccg tgctcgagga
      301 tcgggatcac gtcgggcgcg gtgttcttcg gaaccccggc cgccggagtc ggttcggcct
      361 tcggcgccgg ctgcagcctg atgaccatcg acgggatgcg ctcgatctcg gagagctgaa
      421 ccgtcgtcct gtacggaagg tagccatcgg cctcgacgtc gatgtcccac gctccgcccg
      481 cgaggccgag gtacgcccag cgcccgttct tgtcggacgc gatcgcctcc gggcccgcgc
      541 cggcccgctt gaacacgagc ttcgccccgg gaacgccttc ccccttctcg ttgagaacgc
      601 ggccgtcgac ccgcgccctg ccccgccagt cctgcgcgcg gcccgcgccg gcgaagagca
      661 gcgcgagcac cgccgccgaa gcaatcttcg attgaaatct cacatcaccc tcctcgaaac
      721 gccccgcaca aaacaagagg cctggcggga accattcccg ccggaccgga tcttatcggg
      781 gtcgaaggat ccggtggcgc acccccgagc cgcgaaacgc cgtggcgagt cgtctgctag
      841 catcgccgca tgacactcga tcgagagatc taccgccgcc gactcgagtc gatgcggcaa
      901 aggctcgcct ccgtcgccgc cgacgggctg tacgcgacgc cgtcgtcgaa cctcttttac
      961 ctcacgggga tcgacttctg gaggagcgag cggttgacgg ccctgctgct tttcccggac
     1021 cgggagccgg tcgtgctctg ccccgcgttc gaggaggctc gccttcgcgg gatgtcggcg
     1081 gtgacgaacg tcgtgacgtg ggaggagacc gaggacccct tcgcgaaggc ggcggcgctc
     1141 ttcccggcgg ccgccggcac cctcgcagtg gaaccgtcga ccgcgttcga agacgtcgaa
     1201 cggctgctgg cgcaccggcc gtcgtggaag cccgtctcgg ccggacccgc gatcgcctcc
     1261 gtgcgcatga tcaagacgcc cgaggagatc gccgcgatgc ggcgcgcgat cgcgatcgcg
     1321 ctgccccgct tccgccgcgc cttcgaatcc ctcgctccgg gcgcgaccga ggcgcagatc
     1381 tcgcgcgcct tcggaggcga caacgtcgtc cagttcggcc cctcgtcggc gctccctcac
     1441 ggcgcttcga ccgagcggcc actggcgcgc gacgaggcgg tgttgatcga cgcgtgggac
     1501 aagccggagg gctactacta cgacatcacg cgctcgacct tcttcggtcg gccgcccgac
     1561 gagtaccgga agatctggga gatcgttctc gaggcccagg gggccgcgat cgaccgggcc
     1621 gctccgggag ttccgtgctc cgaggtggac gccgcggcgc gccgggtcat cgagaaagcc
     1681 gggtacggcg cgtacttcac gcaccgactc ggccacggac tcgggatcga cgtccacgag
     1741 ccgccgtaca tggtcgcgca cgagaagacc ctgctcgagc cggggatgac tttcacctcc
     1801 gaacccggga tctacctccc cgggcggttc ggggtacgaa tcgaggacga catcgtttgc
     1861 accgagaccg gcgccgagtc gctttcgccg cgagtcgacc gcctcgagcc aatcgccgcc
     1921 tgacggcctc ggcccgccgc gctctcgcct gcttcgccgc cgcgatcctc gcgtcggcag
     1981 gcgttctcgt gggcgagttc tcagccgcgg aggtggagga ggcttccgag gtcggtcgcc
     2041 gggccgccgt gctcctcctg cacggcgacc ggctatcgat cgaaaacacg ttcgccaccg
     2101 aagccgttgc ctcgaagtac ctcggcgacg acgtgtgggg acggctctcg cagcgccagc
     2161 gtcaggtcat ccgctcgatc gtcctcgacc gcttccagga ggccctcatt ccgccgcgcg
     2221 gaaccccggc ggagattgcg tggtcgtccg cccgaccggc cggagacacc gttcttctct
     2281 tcctgggtct gcggtatccg gcgggagtgc tcaagacgcg ctggtcgctc gcgcgcttct
     2341 cgtcggaatg gaaggtgctg gacgtgcttc tctccgaccc cggtatttcc ctggctgcgg
     2401 aggcggtgcg acctttcggc cgggacgggg tccggccgcg aaattccggc cgcgaggcga
     2461 gggccgccgc acttcctcgc gctctgggga tcgtcgccat cctggcggtc gtgctcttcc
     2521 tcggccggcg cctcgccccg tcctcgcgga agatcctcgt ctggaccgcg gccgccccgg
     2581 cggtgctttt cgccgtggac ggattcttcg cgatccgacg ggcgctgtcc gagccgtatc
     2641 ggattccgga agtgctcgcg cccgccccct ggagcgtcgc cgagcggcag gctctccttg
     2701 cccagcgcga ggggcggctc gacgccgcgc gccgaaactg gcaggcggcc gtcgccgccg
     2761 gcgcgcccgc ggccgccgcg gactatcagg tcgggctcgc gctggcggcc gccggtcgcc
     2821 gggaggaggc gaaagccgcc ttccttcgct ctctctcacg gt
//
LOCUS       DASWAV010000447         2126 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U2665
            k141_705398, whole genome shotgun sequence.
ACCESSION   DASWAV010000447 DASWAV010000000
VERSION     DASWAV010000447.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960316
            Sequence Read Archive: SRR10260007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2126)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2126)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:47:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,929
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2126
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_705398"
                     /isolate="SMAG_U2665"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.36444 N 132.31889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1250
                     /locus_tag="VF958_09255"
     CDS             <1..1250
                     /locus_tag="VF958_09255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9149333.1"
                     /translation="TATRTWPLLYLYDPRKRGALAAERFREAAERYGWILASSNNTMS
                     DGPMAPNVAAINAMWADASRRFPVDPRRVYAAGFSGGARTACLLAQKMDGRVAGVIAC
                     GGGFADGSPPEKGMPFAVFGTVGNVDFNYGEMRRLDRTLAKLGAKHRLAIFDGPHSWC
                     PTPVCTEGVEWMELAAIKSGARPKDSALVERLLRERLERAAALEGAGRAVDAFLRYTG
                     AVEDFTGLTDTKSADAARMRLEELPAVRKELADEERREEKEARSFERLWSDLKAALGS
                     DPPAPLKAIVNQVQVGRLRRDAGPGRPEPDRLSAARVLEQLYVQTAFYLPRDFASKHD
                     FLRAELSTGIAEELKPDRAGALWYDLACFRAVAGDRRGALGSLRTAVKKGFRQVDLIE
                     TDPDLASLREEKDYRAILEELKR"
     gene            complement(1272..>2126)
                     /locus_tag="VF958_09260"
     CDS             complement(1272..>2126)
                     /locus_tag="VF958_09260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007809743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEX9149334.1"
                     /translation="RRAKEMLIGGGGILELVAAPEGFDRVGGLERLKKWVETRKAGFT
                     DAGDGLLDPPRGMLLLGVQGCGKSLAAKAVAACWGVPLFSLEAGRLLAPYVGESERNL
                     REALKRVERMAPCVLWIDEIEKAFVSAHSAESDGGVSQRLLGALLTWMQERRRRVFLV
                     ATCNSVEELPPELMRKGRVDEVFFVDLPGTAARVEIFRMHLEKRGEDPSRFDLAKLAA
                     AADGFSGAEIEQSVVSGLYEARAAGLPLDGAALLVALRSTRPLSIVRAEDVAALRAWA
                     SGRCVPAD"
BASE COUNT          342 a          743 c          739 g          302 t
ORIGIN      
        1 acacggcgac gcggacctgg ccgctcctct acctctacga tccgcgcaaa cgcggcgcgc
       61 tcgcggccga gcgctttcgc gaagccgccg agcgctacgg ctggatcctc gcgagctcga
      121 acaacacgat gagcgacggc cccatggcgc cgaacgtcgc ggcgatcaac gcgatgtggg
      181 cggacgccag ccgtcggttt ccggtcgatc cgcgccgcgt ctatgcggcg ggtttctcgg
      241 gaggcgcccg cacggcgtgc ctgctcgcgc agaagatgga cggcagggtc gcgggcgtga
      301 tcgcgtgcgg gggcgggttc gccgacggga gtcctccgga gaaggggatg cccttcgccg
      361 tcttcgggac ggtcgggaac gtcgacttca actacggcga gatgcgacgt ctcgaccgta
      421 cgctcgcgaa actcggggcg aagcatcgcc tcgcgatctt cgacggtccc cactcctggt
      481 gccccacacc cgtctgcacg gaaggggtcg agtggatgga gctcgcggcg atcaagagtg
      541 gcgcgcggcc gaaagactcc gcgctggtcg agcgcctcct ccgggaacgg ctcgagaggg
      601 ccgcggctct cgaaggcgcc ggaagggcgg tcgacgcctt cctccggtac acgggagcgg
      661 tggaagactt cacgggcttg accgacacga agagcgccga cgcggcgcgc atgcggctgg
      721 aagagcttcc cgcggttcga aaggagctcg cggacgaaga gcgccgggag gagaaggagg
      781 cccgctcctt cgagcggctc tggtcggacc tgaaagcggc gctcggctcc gatcctccgg
      841 cgccgttgaa ggcgatcgtg aaccaggtcc aggtcggaag gctccgtcgg gacgcgggcc
      901 cgggacggcc ggaacccgac cggctgtccg ccgctcgcgt tctcgagcag ctctacgtcc
      961 agaccgcttt ctacctgccc cgggacttcg cgtcgaagca cgacttcctc cgcgcggagc
     1021 tctcgaccgg gatcgcggag gagttgaaac ccgatcgcgc cggcgcgctc tggtacgacc
     1081 tcgcctgctt tcgcgccgtc gccggcgatc ggaggggcgc ccttgggtca ctgcgcacgg
     1141 cggtgaagaa gggttttcgg caggtcgatc tcatcgagac ggaccccgac ctcgcgtcgc
     1201 tgcgggagga gaaggactac cgcgcgattc tcgaggagct gaagagatga ccccgttgcg
     1261 agggagcccg ttcagtccgc gggcacgcag cgcccggatg cccacgcacg cagggcggcg
     1321 acgtcctcgg cgcgtacgat cgacagcgga cgggtcgagc ggagcgcgac gaggagcgcg
     1381 gctccgtcca gaggcaatcc ggcggcccgc gcttcgtaca gtcccgacac gacggactgc
     1441 tcgatctcgg cgcccgagaa cccgtccgcg gccgctgcga gcttggcaag gtcgaaccgc
     1501 gatgggtcct ccccgcgttt ttcgaggtgc atgcggaaga tctccacgcg cgcggccgtc
     1561 cccggcaggt ccacgaagaa aacctcgtcg acgcgccctt tgcgcatgag ctcgggcggg
     1621 agttcctcga ccgagttgca cgtcgccacg agaaaaacgc ggcggcgcct ctcctgcatc
     1681 catgtcagga gcgcgccgag gagccgctgc gacacgcctc catcggactc cgcggaatgc
     1741 gccgacacga aggccttctc gatctcgtcg atccagagca cgcacggagc catccgctcg
     1801 acccgcttca acgcttccct caggttgcgc tcggactcgc cgacgtaggg cgccagcagc
     1861 cgacccgcct cgagggaaaa gagaggcacg ccccagcacg cggcaacggc cttcgccgcg
     1921 agactctttc cgcacccctg gacgccgagg aggagcatgc cgcgcggcgg atcgagaagg
     1981 ccgtcccccg cgtccgtgaa tccggccttc cgggtctcga cccacttctt caggcgctcg
     2041 aggccgccga cgcggtcgaa gccttcgggg gccgcgacga gctcgaggat cccgccccct
     2101 ccgatgagca tctccttcgc tcggcg
//
LOCUS       DASWAV010000448         4533 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U2665
            k141_745460, whole genome shotgun sequence.
ACCESSION   DASWAV010000448 DASWAV010000000
VERSION     DASWAV010000448.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960316
            Sequence Read Archive: SRR10260007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 4533)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4533)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:47:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,929
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4533
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_745460"
                     /isolate="SMAG_U2665"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.36444 N 132.31889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..349
                     /locus_tag="VF958_09265"
     CDS             <1..349
                     /locus_tag="VF958_09265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9149335.1"
                     /translation="KTLPRLAAVPVEKMVPGHGTIGPTTGISDTLAYLRGLDEIVKRL
                     LRAGTPEEYLEIRLREPDNRIPNVPLSEAHLRNAVAVFHREKEKLTKPAATPTRPGRA
                     PSPRPTPRRATGS"
     gene            362..1138
                     /locus_tag="VF958_09270"
     CDS             362..1138
                     /locus_tag="VF958_09270"
                     /inference="COORDINATES: protein motif:HMM:NF012596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HEX9149336.1"
                     /translation="MPLRLKTTAGLAELEIDIPPVNVLGLAALEEFTRLVLQAGGERV
                     LLLRGLPRAFSAGFSIAEHVPDPDSIEAMLVAMRGALNALVKTPAVTVAAVSGACLGG
                     GAELVAACDVVLVAEDARIGFPEIRLACFPPGAAALLPVRIGGARAADWILTGRILSG
                     REAVSAGFAARSFRPADLEMETGRIVRQFVGTSGAALAGARDLLRAERRRALAEALPH
                     AEDAYRLLAGNEDLARAVANFKVGERKTEDGGRKREIGED"
     gene            1242..2093
                     /locus_tag="VF958_09275"
     CDS             1242..2093
                     /locus_tag="VF958_09275"
                     /inference="COORDINATES: protein motif:HMM:NF013247.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class IV"
                     /protein_id="HEX9149337.1"
                     /translation="MIVYLNSRFVPSEEAHLSPEDRGFLFADAIYEVVRYGGGRPYRL
                     AEHLERMRDGLTAIRIAADPQFFPAVASRLLAENGLENDDALVYAQVSRGVAPRYHAF
                     PPEGTPPTIFAFARRTDPAPPLEGGRAILVPDDRWGRCDVKSVMLLANVLASQKAREA
                     GAIEAVLVRDGIAWEGTKSNLFARVGRVLRTAPRGPRILPGVTRAATLEAAAALGESI
                     EERPISIEELFSADEVFLASTTLWTYPVVEIDGRRIGDGRPGPLATRLKETLQAEFHG
                     ARAGLAT"
     gene            2110..2493
                     /gene="queF"
                     /locus_tag="VF958_09280"
     CDS             2110..2493
                     /gene="queF"
                     /locus_tag="VF958_09280"
                     /EC_number="1.7.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014100805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033739 - preQ1 synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preQ(1) synthase"
                     /protein_id="HEX9149338.1"
                     /translation="MADYTEEHARAGLESPLPSLETWRNQYPDYEITVEIPEYNAICP
                     KTGLPDFGHLTIRYVPDELCLELKSLKLYVVAYRNVGIFYENAVNRILRDCVAACRPR
                     RMTVIGRFSSRGGISSVVEARYPAA"
     gene            2498..3142
                     /locus_tag="VF958_09285"
     CDS             2498..3142
                     /locus_tag="VF958_09285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020739477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HEX9149339.1"
                     /translation="MDWLKDLFHQLTDVETLIRLGGLTAMTAIVFAETGLLIGFFLPG
                     DSLLVTAGVFAATGKLNLWHVIGLLSVAAIVGDTVGYWFGRRVGQALFTRPRSLLFNP
                     DHLRRAHDFYEKHGGKTIIIARFMPIIRTFAPIVAGMGQMNYRRFVSFNVVGGILWVI
                     SMTGIGYFLGQIPGVREHIEIVIVVVVFLSILPGIIAGGREWMKKRRAGGASAT"
     gene            complement(3139..3660)
                     /locus_tag="VF958_09290"
     CDS             complement(3139..3660)
                     /locus_tag="VF958_09290"
                     /inference="COORDINATES: protein motif:HMM:NF024273.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HEX9149340.1"
                     /translation="MTKRELETLLLTLESTPALLARAAAELADDRVRHRGTAGGFSLV
                     EHVWHLADLEREGYAARIRRLLTEDEPLLSNFDGGRVARERLYHRRDLAEGLLAFTAA
                     RTRNVQALREIAPSDWKRTGELEGLGTVSLSDVPRLMAEHDREHGIEIAELVRELRDG
                     SAPGRLRPVSAVA"
     gene            3797..4255
                     /locus_tag="VF958_09295"
     CDS             3797..4255
                     /locus_tag="VF958_09295"
                     /inference="COORDINATES: protein motif:HMM:NF016955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerB family tellurite resistance protein"
                     /protein_id="HEX9149341.1"
                     /translation="MGGLDAIRSFFSSKKSPAQPGPGSDPVAIAACALLLEIAHADEN
                     FTSEERERIARHAREDLGVPAEDVREVLRLAEEARRESVDLYQFTKLVTEGFSREQRL
                     RLIEAIWGVVYSDGVLTAVEGQLARRIAELLGFQHPEVQAARERVAARKS"
     gene            4270..>4533
                     /locus_tag="VF958_09300"
     CDS             4270..>4533
                     /locus_tag="VF958_09300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9149342.1"
                     /translation="MGRNPFFLGFLAIWILAWAVLIADDGRTLPLAPKPAHYLVAEGV
                     ALVLVLVLLRLARDRRPIDHESGLPIRQPAAEGAGILLYLFLLV"
BASE COUNT          700 a         1524 c         1534 g          775 t
ORIGIN      
        1 gaaaacgctt ccgcggcttg ccgctgttcc ggtcgaaaag atggtgccgg gccacggcac
       61 cattggcccg acgaccggga tttcggacac cctcgcgtac ctgcgcggcc tggacgagat
      121 cgtcaagcgt ctcctgcgcg cgggcacgcc cgaggagtat ctcgagatcc gccttcgcga
      181 gccggacaac cggatcccga acgtgccgct gtccgaggcg cacctgagga acgccgtcgc
      241 ggtcttccat cgcgaaaagg agaagctgac gaagcccgcg gcgacgccga cccgcccagg
      301 gcgggctccg tcgccccgtc cgactccacg ccgcgccacg ggttcgtgag ataagaaggg
      361 cgtgcccctc cgcctcaaga ccacagcggg gctcgccgaa ctcgagatcg acatcccgcc
      421 ggtcaacgtc ctcgggctgg cggcgctcga ggagttcacc cggctggtcc tgcaggcggg
      481 aggcgagcgc gtcctcctcc tgcgaggatt gccgcgggcc ttctcggccg gcttctcgat
      541 tgccgagcac gtccccgacc ccgactcgat cgaagcgatg ctggtggcca tgcgtggcgc
      601 tctgaatgct ctcgtgaaga cgcctgccgt cacggtcgcg gccgtctccg gagcgtgtct
      661 cggcggcgga gcggagctcg tggccgcgtg cgacgtcgtc ctcgtcgccg aagatgcgcg
      721 aatcgggttt ccggaaatcc gcctcgcgtg ttttccgccc ggcgccgccg cgctcctgcc
      781 cgtccggatc ggcggagcgc gcgccgccga ctggattctg accggacgga tcctgtcggg
      841 gcgggaggcc gtttcggcag gattcgccgc gcgctctttc cgacccgcgg atctcgagat
      901 ggagaccggg cggatcgtcc gacagttcgt cggcacgagc ggcgccgcgc tcgccggcgc
      961 gcgcgacctg ttgcgggcgg aaaggcggcg ggcgctggcc gaagcgctgc cgcacgcgga
     1021 ggatgcctat cggttgctcg ccggcaacga ggatctggcg cgcgcggtcg cgaacttcaa
     1081 ggtcggagaa cggaagacgg aagacggagg acggaagaga gagatcgggg aagattgaga
     1141 gcgccgaggc cgaaccaaac ccctcacccc cggccccctc tcctggaggg agagggtgca
     1201 accttcggcg atcaagggat gcccgtttac aatccccgcc cgtgatcgtc tacttgaaca
     1261 gccggttcgt cccgagcgag gaagcgcacc tttcgccgga agaccgcggg ttcctcttcg
     1321 cggacgccat ctacgaggtc gttcgctacg gcggcggtcg tccgtaccgc ctggcggagc
     1381 acctcgagcg aatgcgcgac ggtctgacgg cgattcggat cgccgcggat ccgcagttct
     1441 ttccggcggt cgcgtcgcgg ctacttgccg agaacggact cgagaacgac gacgcgctcg
     1501 tatatgcgca ggtctcccgc ggtgtcgcgc cgcgctatca cgcgtttccg ccggagggca
     1561 cgccgccgac gatcttcgct ttcgcccggc gcacggatcc tgctccgccg ctcgagggag
     1621 ggcgcgcgat cctggtcccc gacgaccggt ggggacgctg cgacgtcaag agcgtgatgc
     1681 tgctcgcgaa cgtgctcgct tcgcagaagg cgcgcgaggc gggcgcgatc gaggccgtgc
     1741 tcgtccgcga tggaatcgcc tgggagggga cgaagtcgaa tctcttcgcg cgcgtgggtc
     1801 gagttctgcg cacggcgccg cggggtccgc ggatcctccc gggtgtgacc cgggcagcga
     1861 cactcgaagc ggccgccgcc ctgggcgaat cgatcgaaga gcggccgatc tccatcgagg
     1921 agctgttttc cgccgacgag gtcttcctcg cgtcgacgac cctgtggacc taccccgtag
     1981 tcgagatcga tggccgtcgg atcggcgacg ggcgtcccgg cccgctcgcg acgcgcctga
     2041 aggagacgct gcaggcggag ttccacggag cccgcgccgg gctcgcgacg tagacgatcg
     2101 cacggggcca tggccgatta caccgaagag cacgcgaggg cgggactgga gtcgccgctc
     2161 ccctctctgg agacgtggcg caatcagtat cccgactacg agatcacggt cgagatcccg
     2221 gaatacaacg cgatctgtcc gaagaccggg ttgcccgact ttggacacct cacgatccgc
     2281 tacgtcccgg acgaactctg tctcgagttg aagtctttga agctctacgt cgtcgcctac
     2341 cgcaacgtcg ggatcttcta cgagaacgcg gtcaaccgca tcctgcgcga ctgtgtcgcg
     2401 gcttgccgac ccaggcggat gaccgtgatc ggccggttct cctcacgggg cggaatctcc
     2461 tccgtcgtcg aagcgcgtta cccggccgcc tgacccgatg gactggctga aagacctctt
     2521 ccaccaactg acggacgtcg agaccctcat ccgattgggg ggactcaccg cgatgacggc
     2581 catcgttttc gccgagacgg gactgctcat cgggtttttt ctcccgggcg attcactgct
     2641 cgtgacggca ggtgtcttcg cggccacggg caagctcaac ctgtggcacg tcatcggcct
     2701 tctctcggtc gccgcgatcg tcggggacac cgtcggctac tggttcggcc ggcgcgtggg
     2761 gcaagcactc ttcacgcggc cgagatccct cctcttcaat cccgatcacc ttcggcgcgc
     2821 gcacgacttc tacgagaagc acggcggaaa gacgatcatc atcgcccgtt tcatgcccat
     2881 catccgcact ttcgcgccga tcgttgccgg catgggacag atgaactatc gacgattcgt
     2941 gtcgttcaac gtcgtcggag ggatcctgtg ggtgatctcg atgacgggga tcggctactt
     3001 cctgggccag attccgggag tgcgcgagca catcgagatc gtcatcgtcg tcgtcgtgtt
     3061 tctctcgatc ctgcccggca tcatcgccgg gggtcgagag tggatgaaga agcggcgagc
     3121 ggggggcgcg agcgcgactt aggccaccgc cgagacggga cgtagccttc ccggcgcgga
     3181 cccgtcgcgg agctcccgga cgagctcggc gatctcgatg ccgtgctccc ggtcgtgttc
     3241 ggccatcagc cgcgggacgt cggagagcga aaccgtgccc aggccctcca gctcgccggt
     3301 ccgtttccag tccgaaggcg cgatctcgcg cagcgcctgg acgtttcgcg tgcgggccgc
     3361 ggtgaacgcg agcagaccct cggccagatc gcgccggtga tagaggcgct cgcgcgcgac
     3421 ccggccgccg tcgaagttcg aaagcagtgg ttcgtcctcc gtgagcagcc gccggatgcg
     3481 ggcggcgtag ccttcgcgct cgaggtcggc gagatgccag acgtgctcga ccagcgaaaa
     3541 gcctcccgcc gtgccgcggt ggcgcaccct gtcatctgcg agctccgccg cggcgcgggc
     3601 gagaagcgcc ggagtcgatt cgagagtgag gaggagagtt tcgagttcgc gcttcgtcat
     3661 gatcgtgtgt tccaaccttc cagtacgcat tcgacgagcg aaaagtttcc cggttagagg
     3721 gttccttaat gcgacctcga gagccgggca gcgagtgatt actccctttt ccggagcaaa
     3781 ccgctagact cgacccgtgg gcggcctcga cgcgattcgg tccttctttt cctcgaagaa
     3841 gtccccggca cagccgggtc ccggcagcga cccggtcgcg atcgcggcgt gcgcgctgct
     3901 tctcgagatc gctcacgccg acgagaactt cacgtccgag gagcgggagc gaatcgcgcg
     3961 gcacgcgcgc gaggacctcg gtgtccccgc ggaagacgtt cgtgaagttc tccgtctcgc
     4021 cgaagaggct cggcgcgaaa gtgtcgacct ctaccagttc acgaagctcg tcacggaagg
     4081 gttctcccgg gagcagcgcc ttcggttgat cgaggcgatc tggggcgtcg tctattccga
     4141 cggcgtgctg acggcggtgg aagggcagct cgcgcgtcgc atcgcggagc tgctcggctt
     4201 ccagcacccg gaggtgcagg ccgctcgcga gcgcgtcgcg gcgcgcaaga gctgacgccc
     4261 gccggcgtca tgggacggaa cccgtttttc ctcgggttcc tcgcgatctg gattctcgcg
     4321 tgggcggtgc tgatcgcgga cgacggccga acgcttccgc tcgcaccgaa gccggctcac
     4381 tacctcgtcg cggagggggt cgcgctcgtt ctggttctcg tccttctccg cctcgcgcgt
     4441 gatcgccgcc cgatcgacca cgagagcggt cttcccatcc ggcagcccgc ggcggagggc
     4501 gcggggatcc tgctctatct tttccttctc gtc
//
LOCUS       DASWAV010000449         2464 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U2665
            k141_745474, whole genome shotgun sequence.
ACCESSION   DASWAV010000449 DASWAV010000000
VERSION     DASWAV010000449.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960316
            Sequence Read Archive: SRR10260007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2464)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2464)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:47:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,929
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2464
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_745474"
                     /isolate="SMAG_U2665"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.36444 N 132.31889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(55..483)
                     /locus_tag="VF958_09305"
     CDS             complement(55..483)
                     /locus_tag="VF958_09305"
                     /inference="COORDINATES: protein motif:HMM:NF025887.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4440 domain-containing protein"
                     /protein_id="HEX9149343.1"
                     /translation="MNPDADEVRRIIEGHNADAVRWYAAGDADSLANLFAVKAWQMQP
                     NAPPLVGREMIRAFWKNALNWGSWNFELETRDVLVSGPLAVERGRYRLRFTEKPGVPP
                     AMPSLEDRGNYVVLWRRDDDGEWRVLWDAPVSEKTLVVTR"
     gene            complement(480..992)
                     /locus_tag="VF958_09310"
     CDS             complement(480..992)
                     /locus_tag="VF958_09310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011525344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HEX9149344.1"
                     /translation="MKLNELLLGELEREAPASRRILERVPDGRYDWKPHEKSMPLGYL
                     ATLVATMPGWIDAMVNLDQLDFAPKEPSAYKPPELRTNRELLEAFDAALAKGHAALRA
                     TNDEHLRTTWKLLAGGEVVQEQPRYIAIGDGVLNHWAHHRGQLSVYLRLNDVPVPSIY
                     GPTADEGIEQ"
     gene            1087..1854
                     /locus_tag="VF958_09315"
     CDS             1087..1854
                     /locus_tag="VF958_09315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein-sorting protein"
                     /protein_id="HEX9149345.1"
                     /translation="MMRSLVLGLAASLALAGPSAAQTPAPTVDEIVAKNIQARGGLEK
                     IKAVKSMRVTGHMTVGPGTEAPMVIEAKRPDKLRLDITLQGMTLSQAYDGKVGWQINP
                     FGGGSKNAEPMSAEELKEAAEQADMDGPLVDWKAKGHKVELVGKEKLEGTDVYKLKVT
                     MKDGNVHYLFLDADSYLDIKGEAKRTVRGTEVETEQTIGDYKEVGGLMVPHSFESGAK
                     GRPEKQKMTIEKVELDAPIEDARFAMPAKKDEPAPKK"
     gene            1864..>2464
                     /locus_tag="VF958_09320"
     CDS             1864..>2464
                     /locus_tag="VF958_09320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017668385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HEX9149346.1"
                     /translation="MLALSLRRRLALALLALSGALTAVAAGQETKIDSDALGGLEARL
                     IGPAVMSGRIAAIDAVPGDRLTVWVGAASGGVWKSVDGALTFKPVFDEGGKSQSIGAV
                     RIDPKDPKTVWVGTGETWTRNSVSVGDGVYKTTDGGDNWTRLGLENTERIARIAVDPK
                     DSNVVFVCATGHLFDDHPDRGVYRTKDGGKTWDKVLFVAN"
BASE COUNT          438 a          856 c          783 g          387 t
ORIGIN      
        1 ccaccgccgc ccactcgcgg tcgcgttcca ggagcgcagc cttttcatcg cggctcatcg
       61 cgtcacgacc aatgtcttct cgctgaccgg cgcgtcccaa aggacccgcc actccccgtc
      121 gtcatcccgt cgccagagaa ccacgtagtt cccccggtcc tcgagcgaag gcatcgccgg
      181 cggcacgccc ggcttctcgg taaatcgaag ccgatatctc cctcgctcga ccgcgagcgg
      241 cccgctcacg aggacgtctc gagtctcgag ctcgaaattc cagctccccc agttgagcgc
      301 gttcttccag aaggcccgga tcatctcccg ccccaccaga gggggcgcgt tcggctgcat
      361 ctgccaggcc ttgacggcga agaggttcgc gagcgaatcg gcatcgcccg ctgcgtacca
      421 gcgcaccgca tccgcgttgt ggccctcgat gatgcgccga acctcgtccg cgtccggatt
      481 cattgctcga tcccctcgtc cgccgtcggg ccgtagatcg acggaaccgg cacgtcgttc
      541 aaccgcagat acacgctcaa ctggccgcgg tggtgcgccc agtggttcaa cacgccgtcg
      601 ccgatcgcga tgtaccgcgg ctgctcctgc acgacctcgc cgccggccag cagcttccag
      661 gtcgtccgca ggtgctcgtc gttcgtggct ctcaaggcgg catgaccttt cgcgagcgcg
      721 gcgtcgaacg cctccaggag ctcgcggttc gtccgcagtt ccggcggctt gtacgcggac
      781 ggttccttcg gcgcgaagtc gagctggtcc aggttcacca tcgcgtcgat ccagcccggc
      841 atcgtggcca cgagagtcgc caggtatccc agcggcatgg acttctcgtg cggcttccag
      901 tcgtagcggc catccgggac ccgctcgagg atccggcggc tcgcgggcgc ttcgcgctcg
      961 agctcgccca gaaggagttc gttcagcttc atcggttgct ccttgatccg aatctgcgtc
     1021 ccgatcctag tccccggcgt cctctccctc ccgtcatatg ctctgccagc caaaaggagg
     1081 cctcccatga tgcgatccct cgtgctcggc ctggcggcca gcctcgccct cgccggcccg
     1141 tccgccgcgc agacccccgc acccaccgtc gatgaaatcg tcgcgaagaa cattcaggcc
     1201 cgcggcggcc tcgagaagat caaggccgtc aagagcatgc gcgtgaccgg ccacatgacc
     1261 gtcgggccgg ggacggaagc cccgatggtg atcgaggcca agcggccgga caagctgcgc
     1321 ttggacatca ccctccaggg aatgacgctc tcgcaggcct acgacggcaa ggtcggctgg
     1381 cagatcaatc ctttcggtgg aggcagcaag aacgccgagc cgatgtcggc cgaggagctg
     1441 aaggaggccg cggagcaagc cgacatggat ggtccactgg tggactggaa ggcgaagggg
     1501 cacaaggtcg agctcgtcgg caaggagaag ctcgaaggca ccgacgtcta caagctgaag
     1561 gtgacgatga aagacggcaa cgtgcactac ctctttctcg acgccgactc ttatctcgac
     1621 atcaaggggg aggcgaagcg caccgtccgc ggaaccgaag tcgaaaccga gcagacgatc
     1681 ggcgactaca aggaagtcgg cggtctcatg gtcccccact ccttcgaatc gggcgcgaaa
     1741 ggcaggcccg agaagcagaa gatgacgatc gagaaggtcg agctcgacgc cccgatcgag
     1801 gacgcccgtt tcgcgatgcc ggcaaagaag gacgagccgg ccccgaagaa gtagggcccg
     1861 aagatgctcg cgctttcgct ccgtcgtcgc ctggcattgg ctctcctggc gctctccggc
     1921 gcactcaccg cggtcgccgc cggccaggag acgaagatcg attccgacgc gctgggcggt
     1981 ctcgaggctc gcctgatcgg ccccgcggtg atgagcggac gcatcgcggc catcgacgcg
     2041 gtccccggcg accgtctgac cgtctgggtc ggcgccgctt ccggcggcgt ctggaagtcg
     2101 gtcgacgggg ccctgacttt caagcccgtc ttcgacgaag gcggcaagag ccagtcgatc
     2161 ggcgccgtca ggatcgaccc gaaagacccg aagaccgtct gggtcgggac cggcgagacg
     2221 tggacccgca acagcgtctc ggtgggcgac ggcgtctaca agacgaccga cggcggcgac
     2281 aactggaccc ggctcgggct cgagaacacc gagcgcatcg cgaggatcgc cgtcgacccg
     2341 aaggattcga acgtcgtttt cgtctgcgcc accggacacc tcttcgacga ccacccggac
     2401 cgcggcgtct accggacgaa ggacggcggc aagacgtggg acaaggtgct cttcgtcgcg
     2461 aacg
//
LOCUS       DASWAV010000450         4913 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U2665
            k141_63860, whole genome shotgun sequence.
ACCESSION   DASWAV010000450 DASWAV010000000
VERSION     DASWAV010000450.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960316
            Sequence Read Archive: SRR10260007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 4913)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4913)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:47:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,929
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4913
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_63860"
                     /isolate="SMAG_U2665"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.36444 N 132.31889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            93..2933
                     /locus_tag="VF958_09325"
     CDS             93..2933
                     /locus_tag="VF958_09325"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX9149347.1"
                     /translation="MRTLFLPPTYQDSAESGRLILRDGSSAFIRLSEPGDREAMREFF
                     HQLSPLSKKRRFFSSGDPAETLLERQCDSSNPRSQLTLIVNRLVEGESRIIATGSYVA
                     RDETTAEVAFAVDDEFQGKGLGGLLLERLALLAVRNGFVRFWALTRVENQLMLETFRK
                     SGFPLHSKVEDECVEIDFSVAPSEDSVNRSEVRDRVFTTASLLPIFRPKSIAFIGASR
                     DTTSLGGRILDSLTRSGFQGPIHAVNPNTDHVGSLATYPSARVIPQGTDLAILAIPRD
                     QVLTAVDDCAAVGIRAVVVITAGFAEADEEGRALQEKLLARVRGHGMRMVGPNSMGLL
                     NADPGVRLNASLFRDLPPHGKVAMSSPSGALGLAMLALAEHRQLGVSTFVNTGNKADV
                     SGNDLLQYWQGDDATRIILLYVESFGNPRRFARVARGLSRSKPIVVVKAERSGADPEA
                     ARPGLPVFIARHDPVNAFFRQTGVIRAETLDEMFDIAAALDNQPLPAGRRVAILSDGG
                     GPGVLCASACRAARLSVSSFSESTTKRLAAFLSSSTTIGNPLELAFPSPPEAYARAVE
                     TLLSAPEVDALIAIHTPLDSNASDAVLEEIRRGTTAGRAAGGAGKPILACLVARGGGG
                     APIALPGDNIPTYAFPESAARVLGKVAAYAEWRAGPLGVIPDFDDIDPASAREICRKV
                     IEERGADWLSVEESTAVLAAMRLALVVGAVARSAGDAAQLARNIGFPVVVKLASRRSQ
                     RLEKGLIHLNLADEAAVERAFEAIRRPVKADDGPEAMEGVLVQPMVASSVEVMVSMAE
                     DPSYGPLIAFGLGGVHVELIADVGVRVTPLTDRAAAAMVREIRGYPLLEGYRGHPPAD
                     VPAIHDVLLRVSRVVEEVPEIAELVLSPIFVGAPGEGCRIGDSRIRVAPPRKGQAARY
                     TTASPSAARSAPAASTPIEDTIVEGAFHDRNG"
     gene            2917..3375
                     /locus_tag="VF958_09330"
     CDS             2917..3375
                     /locus_tag="VF958_09330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012528902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HEX9149348.1"
                     /translation="MTEMADNSWLYRRIVDDSPVAILFADRDGKIRLWNTGAEVMFGY
                     PPEEAIGQSLDLIVPERQRARHWEGWEKVMATGVTKYGHDVLAVPALKKDGSRISIEF
                     NIVLLRAPTGELLGAAAMIQDVSVRWQKQKEMNARIATLEARVAETVKAG"
     gene            3424..4161
                     /locus_tag="VF958_09335"
     CDS             3424..4161
                     /locus_tag="VF958_09335"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX9149349.1"
                     /translation="MEFQRIEDLTAPDGHPVPAFLFLPPSPRGGAVVCHGYGASKEQI
                     LGIGAAVAEKGTAALVIDLCGHGENMAPIGPGMRDELESAVAFVRRFGRTAAIGHSLG
                     GRLALMSSADVMVAMSPSVMVQMSPQGKWMFENLPSPGVREPYSTYVLDLLESLGPVP
                     SHRRPCLLLYSERDIQTLLDGAAALQEQLPLAEKRLISANIRPDVQHENGFIRYLPRW
                     FNHLELRFNSSACETVSDWVSDHLASV"
     gene            4209..4427
                     /locus_tag="VF958_09340"
     CDS             4209..4427
                     /locus_tag="VF958_09340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9149350.1"
                     /translation="MQWEYFDRMVIFSDAEGGLVSGLDEWINGVGTEGWELVSSAPLI
                     APNKDGIAYGTIGLHLIFKRPVGTPQAT"
     gene            4435..4758
                     /locus_tag="VF958_09345"
     CDS             4435..4758
                     /locus_tag="VF958_09345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9149351.1"
                     /translation="MAGLKGPALIESRYGPLGSGGQSLRIRDVDYSVSELLSRMGLNF
                     DDSRAIDASTLAEGRYVFRYYDGQDQRVVALEFDADFQSLGEVRAQFPEWIGDEEYYP
                     QYCGH"
BASE COUNT          823 a         1603 c         1625 g          862 t
ORIGIN      
        1 cgaacgcatc actcgacgct cgcacacggg gcgctaagat ggtttcgact cgcgcttcgg
       61 gaggacatac acggcgcacg ccgcctgcgc tcatgcgaac tctcttcctg ccgccgacct
      121 atcaggactc cgcggaatcc ggtcgtctca ttctgcgcga tggatccagc gcgttcattc
      181 gcctttcgga accgggcgac cgggaggcga tgcgagagtt ttttcatcag ctctcgccgc
      241 tgtcgaagaa gcgacgattc ttttcgagcg gggatccggc ggagacgctg ctcgagcggc
      301 aatgcgattc gtccaacccc cggtcgcagc tgacgctgat cgtgaaccgc ctcgtcgagg
      361 gtgagtcccg gatcatagcc accggctcct acgtcgcgcg cgacgaaacg acagccgagg
      421 tcgccttcgc ggtcgacgac gagtttcagg ggaagggtct gggggggctc cttctcgagc
      481 ggctggccct cctggcggtc cgaaatggct tcgtgcgctt ctgggctctg actcgggtcg
      541 agaaccagct gatgctcgag acgttccgca agtcggggtt tcctctccac tcgaaggtcg
      601 aagacgaatg cgtcgagatc gacttctccg tcgctccgag cgaggacagc gtgaaccgat
      661 cggaggtgag ggaccgggta ttcacaaccg cctcgctcct cccgatcttc cggccgaaat
      721 ccatcgcctt catcggcgcg tcccgcgaca cgacgagcct cggcggcagg attctcgact
      781 cgctcacccg ctccggcttc cagggtccaa tccatgcggt gaacccgaac acggaccatg
      841 tcggatcgct ggcgacctat ccctcggcac gcgtgatccc gcaggggacg gatctggcca
      901 tcctggcgat cccgcgggac caggtgctca ctgccgtgga cgactgcgcg gccgtcggga
      961 tccgggcagt cgtggtcatc accgcgggtt tcgcggaggc cgacgaagaa ggccgcgctc
     1021 tccaggagaa gcttctggcg cgcgtccggg gtcacgggat gcgcatggtc gggcccaatt
     1081 cgatgggcct cttgaacgcc gatcccggcg tgcgcctgaa cgcctccctt ttccgcgacc
     1141 tgccgccaca cgggaaggtc gcgatgtcct ctccgagcgg tgccctcggt ctggctatgc
     1201 tcgccctggc cgagcaccgt cagctcgggg tctcgacctt cgtcaacacc gggaacaagg
     1261 cggacgtctc tggcaacgac cttctgcagt actggcaggg tgacgatgcg acgcgcatca
     1321 tcctcctcta cgtcgaatcc ttcggaaacc cgcggcgctt cgcgcgggtt gcgcgcggcc
     1381 tctcccgatc caagccgatc gtcgtcgtga aggcggagcg cagcggggcg gatccggagg
     1441 cggcccgacc gggcctgcca gtgttcatcg cgcgccacga cccggtgaat gcgttcttcc
     1501 gacagacggg cgtcatccgg gccgagacgc tggacgagat gttcgatatc gccgccgccc
     1561 tcgacaacca gccgcttccc gcaggacgtc gcgtcgcgat tctttcggac ggcggcggcc
     1621 ccggcgtcct ctgtgcgagt gcctgccgcg ccgcgcgcct gtccgtttct tcgttctccg
     1681 agtcgacgac gaagcggctc gccgcttttc tctcctcctc gacgacgatt ggcaatcccc
     1741 tggagctcgc tttcccctcg ccgccggaag cgtacgctcg cgcggtcgag acgcttctct
     1801 ccgctcccga agtcgatgcc ttgatcgcga tccacactcc actcgactcg aatgcttcgg
     1861 acgccgtgct ggaggagatc cggcggggga ccacggcggg ccgggcggcc ggcggcgctg
     1921 gcaagccgat cctggcgtgc ctggtggcga gaggcggcgg cggcgcgccg atcgcgctcc
     1981 ccggcgacaa catacccacg tatgcgtttc cggaatcggc ggcgcgggtg ctcgggaagg
     2041 tcgcggccta cgcggagtgg cgcgcaggcc cgctcggagt catccccgac ttcgacgaca
     2101 tcgaccccgc ttctgcccgc gagatctgcc gaaaggtgat cgaggaacgc ggagccgact
     2161 ggctgtccgt ggaggagtcg acggccgtcc tggccgccat gcgcctggcg ctcgtcgtgg
     2221 gagcagtggc ccgctcggcg ggcgacgccg cgcaactggc tcggaacatc gggtttcccg
     2281 tcgtcgtcaa gctggcctcg cgccggagtc aacgactgga gaaagggttg atccacctga
     2341 acctggccga tgaggccgcg gtcgagaggg cgttcgaagc gattcgacgt ccggtgaagg
     2401 cggacgacgg tccggaggcg atggagggtg tgctcgtcca gccgatggtc gcaagcagcg
     2461 tcgaggtgat ggtgagcatg gcggaggacc cctcctacgg ccccctgatc gcgttcggac
     2521 tggggggagt ccatgtcgag ttgatcgcgg atgtcggcgt gcgcgtgaca cctctgaccg
     2581 accgcgccgc agccgccatg gtgcgtgaga tccggggcta tcctctgctc gaaggctatc
     2641 gcgggcatcc gccggccgac gtcccggcca tacacgacgt cctccttcgc gtctcccgtg
     2701 tggtcgagga agtccccgaa atcgcggagc tcgtactgag cccgatcttc gtgggagctc
     2761 ccggtgaggg ctgccgcatc ggtgactcgc ggattcgcgt ggcgccgccc cgtaagggac
     2821 aggcggcccg ttacactacg gcgtcacctt cggctgcgcg aagcgcgccg gcggcgtcta
     2881 ccccgatcga ggatacgatc gtcgaaggag catttcatga ccgaaatggc tgacaactcc
     2941 tggctgtatc ggagaatcgt cgacgacagc ccggttgcga tcctgttcgc ggatcgcgac
     3001 gggaaaatcc gcctctggaa cacgggagcg gaggtcatgt tcggctaccc gccagaggaa
     3061 gcaatcgggc agtcgctgga cctgatcgtc ccggagcgtc agagggcccg gcactgggaa
     3121 ggctgggaaa aggtgatggc cacgggggtg acgaagtacg gccacgacgt gttggccgtg
     3181 ccggccctga agaaggacgg ctcgcgcata tccatcgagt tcaacatcgt ccttctccgc
     3241 gccccgaccg gcgaactcct gggtgcggct gccatgatcc aggacgtcag cgtccggtgg
     3301 cagaaacaaa aagagatgaa cgcgcgcatc gcgacgctcg aggccagggt ggcggaaacc
     3361 gtcaaggcgg gttgacgaga gaacccctcg cgtcggaggc gaggagtttt cgaggagtgt
     3421 gagatggagt ttcagcggat cgaagacctg accgcccccg acggccatcc tgttcccgcg
     3481 ttcctcttct tgccgccttc gcctcgcggc ggcgccgtgg tctgtcacgg ctacggagcg
     3541 tccaaggaac agatactggg catcggcgcg gctgtcgcgg agaaaggaac ggccgcgctc
     3601 gtcatcgacc tgtgcgggca cggcgagaac atggcgccga tcggcccggg aatgcgcgac
     3661 gagctggaat ccgccgtcgc cttcgtccga cgatttggca ggactgcggc gatcggtcac
     3721 agcctgggag gcaggttggc gctcatgtcg tcagcggatg tgatggtggc gatgtcgccg
     3781 tccgtcatgg ttcagatgtc cccccaggga aagtggatgt tcgagaacct cccgagcccg
     3841 ggcgtgcgag agccctactc gacgtacgtc ctcgacctgc tcgagagcct cgggcccgtt
     3901 ccgtctcaca ggcgaccatg cctcctcctc tactccgagc gcgacatcca gacacttctc
     3961 gacggcgccg cggccctcca ggagcagctc cctctggccg aaaaacgact gatttccgcc
     4021 aacatccgac cggacgttca acacgaaaac ggcttcatcc gctacctccc gcgatggttc
     4081 aatcatctcg agctccgttt caactcctcg gcctgcgaga ccgtgagcga ctgggtgtct
     4141 gatcacctcg catcagtctg aacgcctcga ccgtgtgcta gtctcgcaaa aaagtcggga
     4201 gcgagagaat gcagtgggag tacttcgacc gtatggtcat cttcagcgat gcggaaggcg
     4261 gattggtgag cgggctcgac gagtggatca acggcgtcgg taccgaaggc tgggagctcg
     4321 tctcctcggc cccgctcatc gctccgaaca aggacggcat cgcttacggc acgatcggcc
     4381 tgcacctgat cttcaagcgg cccgtgggga caccgcaggc gacgtgaacg ggtcatggcc
     4441 gggttgaagg gcccggcgct gatcgagagc cgctacggcc ctctcgggag cggcggacag
     4501 agcctgagga tccgggatgt cgactactcg gtttccgagc tgctttcccg catgggcctc
     4561 aacttcgacg acagccgggc catcgacgca tcgacgcttg cggaagggcg ctacgtcttt
     4621 cgctactacg acggacagga ccagcgcgtg gtcgctctcg agttcgatgc cgactttcag
     4681 tctctcggcg aagtgcgtgc tcagttcccg gagtggatcg gagacgaaga gtattacccg
     4741 caatactgcg gacattgacg ggactgccgg cgccgcgcgt gccggctcga tcgatgtgac
     4801 cgtgtggaac ccgaccggga aggtggtgac gagatgagtt acgagaacct ccgcttcgag
     4861 accgatgact ccatcgccgt cgtcaccctg aatcgtccgg acaagcgcaa cgc
//
LOCUS       DASWAV010000451         6105 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U2665
            k141_465288, whole genome shotgun sequence.
ACCESSION   DASWAV010000451 DASWAV010000000
VERSION     DASWAV010000451.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960316
            Sequence Read Archive: SRR10260007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 6105)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6105)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:47:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,929
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6105
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_465288"
                     /isolate="SMAG_U2665"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.36444 N 132.31889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1173
                     /locus_tag="VF958_09350"
     CDS             <1..1173
                     /locus_tag="VF958_09350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9149352.1"
                     /translation="AAGSDGNLWFTENGNTNLAELGRIGRITPAGVITEFPIPTPESQ
                     PIDIAAGPDGNLWFTESYANKIGRITPSGVVTEFIIPTPLSEPISITAGPDGNLWFTE
                     QSANKIGRITTSGVVTEFPIPTPAGSPTGITAGPDAALWFTELSGRIGRLTTGVVGDP
                     TQILPVVGSTPGALGSFFRTSVQLHNAGTTPSTAIIVFHPSGTSGSLELDPVLPVDLA
                     PGETRTIPDLLPAMDRSGLGSADVFLTVGSAGNTPVVSARIFNDAGAAGTTGFALNAL
                     LPEEALAAGRRGVLLVPSDLVAYRMNLGIRTLAANVAIRFTVRNAAGSIAAVVPRFFP
                     AVYHEQQPAEAFLNGLTLPPGGSVTALVESGAAIFYGATVDNRTGDPSLQIARSAP"
     gene            complement(1189..1413)
                     /locus_tag="VF958_09355"
     CDS             complement(1189..1413)
                     /locus_tag="VF958_09355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9149353.1"
                     /translation="MTTNRPSAPPTAEENERADRQPGAKGHPHEKGPSNESPDVQPEE
                     SLRPEHRRSPSGLLDVEHRPGPRDRPQQKP"
     gene            1460..4930
                     /gene="metH"
                     /locus_tag="VF958_09360"
     CDS             1460..4930
                     /gene="metH"
                     /locus_tag="VF958_09360"
                     /EC_number="2.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002637312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008705 - methionine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HEX9149354.1"
                     /translation="MATVEIPKQTGARAAARTEELLAVLADRILVLDGATGTGIQARD
                     LSAADFGGPEYEGCNEHLVLTRPDVVRSIHSGYFEAGADMVETDTFGGTPLVLAEYGL
                     AEKALEINRAAARLAREVAERYRTTERPRFVAGSMGPTTKSITVTGGVTFAEMIRHYR
                     VQATGLLEGGADVLVLETVQDTRNLKAGLIGVEQAFAEVGWRVPVMVSATIEAMGTML
                     AGQSIEAFYASVMHAPLLSVGLNCGTGPEFMTSHLRSLSALARTRVSCYPNAGLPDED
                     GRYCETPEKLVAVMGRFIEQGLVDIVGGCCGTTPAHVRALAAAAAGHRPRRIPDHRKT
                     LVSGIEMLEIDEDNRPVLVGERTNVLGSRKFKRLVAAGEFEAAAEIARAQVKTGAQII
                     DVCLQDPDRDEAADVNAFLERATRMVKVPLMLDSTDASVLELGLEWSQGKSIINSINL
                     EDGEERFRAVVPLAQRYGAALIVGTIDEDPIHGMGVTRERKLEIARRSYTLLTEKYGV
                     PAEDLIFDPLVFPCGTGDEKYVGSAAETIEGIRAIKAEFPECKTILGISNVSFGLPEA
                     GREVLNSVFLYHCVKAGLDLAIVNSEKQMRYPSIPDEERRLCEDLLFDRGEDPIGAFA
                     AFYKAKTARPRTTERPGTALERLPRYIVEGSKEGLVDDIESVRRGGMAPLDIINGPLM
                     SGMREVGRLFNDNELIVAEVLQSAEAMKASVGHLQQFMEKSEGSLKGKIVLATVKGDV
                     HDIGKNLVEIILSNNGYEVINLGIKVASETLVAACRQHRPDAIGLSGLLVKSAQQMVT
                     TAQDLTSAGVTTPLLVGGAALSQRFTDNRIAPAYGGLVVYAEDAMRGLALADRVLSGE
                     EARSALASEAEERRRSRPAVAEREVNPLAAPEVRNATIEILPPLAAPDFEEHVFRDVP
                     LDQVWRYVNPHMLYSKHLGLRGSYWKLKEAGDAKLVELEELADRIRGAGWVRPRAMYR
                     FFEAYSEGNSVSIVSDGRVAGRFTFPRQATGERLCLADFVRPREGGQALDTIALLVTT
                     AGEGVRRRAEDLKHEGEYLLCHALQAIAVETAEGTAEWVHQVIRERWGFPDPPETSMM
                     DRFQARYRGKRYSAGYPAWPELADQTTLFRLLPGRKIGVELTEGFMMDPEASVSALVL
                     HHPQARYFAA"
     gene            5100..5585
                     /locus_tag="VF958_09365"
     CDS             5100..5585
                     /locus_tag="VF958_09365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015337812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HEX9149355.1"
                     /translation="MINDLDRQILTILQKNARTSNAEVARQVGLAPSAVFERIRKLEE
                     RGVLRSYGAQIDPRAVGLPLVAFTFVRSNDRPGGIQTAEKLAEIPEVLEVHHVAGEDC
                     FLVKVRAADTESLGRLLRERLGKIGTITSTRTTIVLETVKETVELPIAVQEEDEEVAS
                     A"
     gene            5578..>6105
                     /locus_tag="VF958_09370"
     CDS             5578..>6105
                     /locus_tag="VF958_09370"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HEX9149356.1"
                     /translation="MLDATTGLESRQGRIIAAFAAVYVIWGSTYLAIRFAIETIPPHL
                     MASARFLVAGAILYAWARLRGAPRPTFSNWRAATIVGGLLLLGGNGAVVWAETRVPSG
                     VTALLVATVPIWMALIEGMRSGGRRPAAAVIVGLALGLGGLALLMAPGNLAGRVDPLG
                     AGALIVGSFSWAFGSL"
BASE COUNT          988 a         2051 c         2082 g          984 t
ORIGIN      
        1 gcggccggga gcgacggaaa cttgtggttc acggaaaacg gcaacacgaa cctggcggag
       61 ctcgggagga tcggccggat cacgcccgcc ggcgtcatca ccgagttccc cattccgaca
      121 ccggagagtc agccgatcga catcgcggcc ggccccgacg gaaacctctg gttcaccgag
      181 tcgtacgcca acaagatcgg ccggatcacg ccgtcgggag ttgtcaccga gttcatcatt
      241 cccacgccct tgagcgaacc gatcagcata acggcgggtc ccgacggaaa tctctggttc
      301 accgagcagt cggcgaacaa gatcggtcgg atcaccacgt cgggcgtcgt caccgagttt
      361 ccgataccga caccagcggg ctcgcccacg ggaatcactg ccggacccga cgcagcgctc
      421 tggttcaccg agctttcggg aagaatcggc cgcctcacga cgggcgtcgt cggcgacccg
      481 acgcagatcc tccccgtcgt cggctcgacg ccgggagcgc tcggctcctt cttccggacg
      541 tccgttcagc ttcacaacgc ggggacgacc ccgagcacgg cgatcatcgt ctttcatccg
      601 tcgggcacga gcgggagcct cgagctcgat cccgtgcttc cggtcgatct cgcgccgggc
      661 gagacgcgga cgatcccgga tctccttccg gcgatggatc gttccggtct gggaagtgcg
      721 gacgtgtttc tgaccgtcgg gtccgcgggg aacaccccgg tcgtctccgc gaggatcttc
      781 aacgacgccg gcgctgccgg aacgaccggg tttgcgctca atgcgctcct tccggaggaa
      841 gcgcttgcgg ctggccgccg tggagtcctg ctcgttccct ccgacctcgt cgcctaccgg
      901 atgaacctcg gcatccggac gctcgccgcg aacgtcgcga tcagattcac ggtgcggaat
      961 gccgcgggct cgatcgctgc ggtcgtgccc cggttctttc ctgccgtcta ccacgagcag
     1021 cagccggccg aggcctttct gaacggactg actcttccgc cgggcggcag cgtgacggct
     1081 ctcgtcgaat ccggcgctgc gatcttctac ggcgcgacgg tcgacaaccg aaccggcgac
     1141 ccgagcctgc agatcgcgcg ctccgcgccg tagcggtcga cgttcgttct agggcttttg
     1201 ctgcggacga tcccgcgggc ccgggcgatg ttcgacgtcc agcagtccgg atggactgcg
     1261 ccggtgctcg gggcggagcg actcctcggg ctgcacgtcc ggtgactcgt ttgacggacc
     1321 cttctcgtgc gggtggccct tcgcgccggg ctggcggtcg gcccgctcgt tctcttcggc
     1381 ggtcggaggc gcggagggcc ggttcgtcgt catgaccctc gacctcgcaa agcccgggcc
     1441 aacgcgctat aaatctcaaa tggcgacggt cgaaatcccg aaacagacgg gcgcccgcgc
     1501 ggcggcgcgg accgaggagc ttctcgccgt cctggccgac cgcatcctcg tcctcgacgg
     1561 cgccaccggc acgggcatcc aggcgcgcga cctctccgcc gcggacttcg gcggccccga
     1621 atacgaaggc tgcaacgagc acctcgtgct gacgcgcccc gacgtcgtcc ggtcgattca
     1681 ctccggctac ttcgaggcgg gcgccgacat ggtcgagacc gacacgttcg gcggaacgcc
     1741 gctcgtcctc gcggagtacg gcctcgcgga gaaggcgctc gagatcaacc gcgcggccgc
     1801 gcggctggcg cgagaggtcg cggagcgcta ccggacgacg gagcggccgc gcttcgtcgc
     1861 cggttcgatg ggtccgacga cgaagtcgat caccgtcacc gggggcgtca cgttcgccga
     1921 gatgatccgg cactaccgcg tgcaggcgac gggactcctc gaggggggcg cggacgtcct
     1981 cgtgctcgag acggtgcagg acacgcgaaa cctgaaagcc gggctcatcg gcgtcgagca
     2041 ggcattcgcg gaagtcggct ggcgggttcc ggtcatggtc tccgcgacga tcgaggcgat
     2101 gggaacgatg ctcgccgggc agtcgatcga agcgttctat gcctcggtca tgcacgcgcc
     2161 gctgctctcg gtcggtctga actgcggcac cggcccggaa ttcatgacga gccatctgcg
     2221 cagtctttcc gcgctggccc gcacccgcgt ctcctgctat ccgaacgcgg ggctgccgga
     2281 cgaagacggg cgctactgcg aaacgccgga aaagctcgtc gccgtgatgg ggcgcttcat
     2341 cgagcaaggg ctcgtcgaca tcgtcggggg gtgctgcggc acgacccccg cgcacgttcg
     2401 ggcgctggcc gccgccgccg cgggccaccg tccgcgccgc attccggatc accgcaagac
     2461 cctcgtctcg gggatcgaga tgctcgaaat cgacgaggac aaccgtcccg tgctcgtcgg
     2521 agagcgcacg aacgtcctgg gcagccggaa gttcaagcgc ctcgtggcgg cgggggagtt
     2581 cgaagcggcc gcggaaatcg cgcgcgcgca ggtcaagacc ggcgcccaga tcatcgacgt
     2641 ctgtctccag gacccggacc gggacgaggc cgccgacgtg aatgcattcc tcgagcgcgc
     2701 gacccggatg gtgaaggttc cgctcatgct cgactccacc gacgcgagcg tgctggagct
     2761 cggactcgaa tggagccagg gcaagtcgat catcaactcg atcaacctcg aggacggcga
     2821 ggagcggttc cgcgcagtcg tgccgcttgc gcagcgctac ggcgcggccc tgatcgtcgg
     2881 aacgatcgac gaggatccga tccacggcat gggcgtgacg cgagagcgga agctcgagat
     2941 cgcgcggcgg agctacacgc tcctgaccga gaagtacggc gttccggccg aggacctgat
     3001 cttcgatccc ctcgtcttcc cgtgcggcac gggggacgag aagtacgtcg gcagcgcggc
     3061 ggaaacgatc gagggcatcc gcgcgatcaa ggccgagttt ccggagtgca agacgatcct
     3121 cggcatctcg aacgtctcct tcggccttcc ggaggcgggg cgcgaggttc tgaactccgt
     3181 ctttctctac cactgcgtga aggcgggact cgacctcgcg atcgtcaact ccgagaagca
     3241 gatgcgctat ccctcgatcc ccgacgagga gcgacgcctt tgcgaggacc tgctcttcga
     3301 ccggggcgag gacccgatcg gcgcttttgc cgctttctac aaggcgaaga cggcgcggcc
     3361 gaggacgaca gagcgtccgg ggaccgcgct cgagaggctg ccgcgctaca tcgtcgaggg
     3421 ttcgaaggaa gggctcgtcg acgacatcga gtccgtgaga cgtggcggga tggcgccgct
     3481 cgacatcatc aacgggcccc tcatgagcgg gatgcgtgag gtcggccggc tcttcaacga
     3541 caacgagctc atcgtcgcgg aggtgctgca gtcggccgag gcgatgaagg cgtccgtcgg
     3601 ccatctccag cagttcatgg agaagtcgga agggtcgctg aagggaaaga tcgtcctcgc
     3661 gacggtcaag ggggacgtcc acgacatcgg caagaacctg gtcgagatca tcctgtcgaa
     3721 caacggctac gaagtgatca acctcggcat caaggtcgcg tcggaaacgc tcgtggccgc
     3781 gtgccgccag catcggcccg acgcgatcgg tctgtcggga ctcctcgtca agagcgcgca
     3841 gcagatggtg acgacggcgc aggacctgac gtccgccggc gtcacaaccc cgctcctcgt
     3901 gggcggcgcg gctctctcgc agcggtttac cgacaacagg atcgcgccgg cgtacggcgg
     3961 gctcgtcgtc tacgcggaag acgccatgcg cgggctcgcg cttgccgatc gggttctctc
     4021 cggcgaggaa gcgcgctcgg cgctcgcgtc ggaggcggag gagaggcgtc gctcccggcc
     4081 cgcggtcgcc gaacgcgagg tcaatccgct ggcggcgccg gaggtccgta acgcgacgat
     4141 cgagatcctg ccgcccctgg cggctcccga cttcgaggag cacgtctttc gcgacgttcc
     4201 gctcgaccag gtctggcgat acgtcaaccc ccacatgctc tactcgaagc acctcgggct
     4261 gcgcggaagc tactggaagc tgaaggaagc cggtgatgcg aagctagtcg agctcgagga
     4321 gctcgcggat cgcatccgcg gggcgggctg ggtccggcca cgcgcgatgt accggttctt
     4381 cgaggcgtac tcggagggaa actccgtcag catcgtctcc gatgggcgcg tggcgggacg
     4441 attcacgttc ccgcggcagg cgacgggcga gcgcctgtgc ctggcggatt tcgtccggcc
     4501 gcgcgaaggc ggacaggcgc tcgacacgat cgcgctcctc gtcacgacgg cgggcgaggg
     4561 cgtccgcagg agagcggaag acctgaagca cgaaggggag tacctcctct gccacgcgct
     4621 gcaggcgatc gcggtcgaga cggcggaagg cacggcggag tgggtccacc aggtcattcg
     4681 cgaacgctgg ggctttccgg acccccccga gacctcgatg atggatcgct tccaggcgcg
     4741 ctatcgcggc aagcggtaca gcgccggata tcccgcctgg cccgagctgg ccgaccagac
     4801 gactctcttc cgtttgcttc ccggccggaa gatcggcgtc gagctgaccg agggattcat
     4861 gatggatccc gaggcgtccg tttccgcgct cgtcctccac catccccagg cgcgctactt
     4921 cgcggcgtaa ggcgtcgggt attcacagct caacggatcg gtgaagacgg accgaggccc
     4981 ttgccatttc acggcgcgag gtggcaagtg gagacctgac ccccggagtg gccgtgtcag
     5041 gtcgtcatga aatggcctct tgaaacggcg attcgtctgt tgtattagtt tatttcagaa
     5101 tgatcaacga tcttgaccgc caaatcctga cgatcctcca gaagaacgct cggacctcca
     5161 acgcggaggt cgcccgacag gtcggtctcg caccttccgc ggtcttcgag cggatccgca
     5221 agctcgagga gcgcggcgtg ctgcggagct acggggccca gatcgacccg cgcgcggtgg
     5281 gcctgccgct cgtcgccttc accttcgtcc gatcgaacga ccggccgggc ggcatccaga
     5341 ccgcggagaa gctcgcggag atccccgagg tcctggaggt gcaccacgtc gcgggcgagg
     5401 actgctttct cgtgaaggtc cgcgccgcgg acaccgaatc gctcggccga ctccttcgcg
     5461 agcggctcgg caagattggg acgatcactt ccacgcgcac gacgatcgtc ctcgagacgg
     5521 tgaaggaaac ggtcgagctg ccgatcgcgg tccaggagga ggatgaggag gtcgccagtg
     5581 cttgacgcga cgaccggtct cgagtcccgg cagggaagga tcatcgccgc tttcgcggcg
     5641 gtctatgtga tctggggatc gacgtatctc gcgatccgct tcgcgatcga aacgatcccg
     5701 ccgcacctga tggcctcggc gcggttcctc gtcgcgggcg cgatcctcta cgcctgggcg
     5761 cgcctgcgcg gcgctccgcg gccgacgttc tcgaactggc gcgccgcgac gatcgtcgga
     5821 ggactcctgc tcctgggtgg gaacggcgcg gtcgtgtggg ccgagacccg cgtcccctcc
     5881 ggcgtcacgg cacttctcgt cgcgaccgtt ccgatctgga tggcgctcat cgagggcatg
     5941 cgcagcggcg gacgccgccc cgccgccgcc gtcatcgtcg gcctcgcgct cggtctgggc
     6001 ggtctcgcgc tgctcatggc gcccggcaac ctcgccggcc gagtggatcc gctcggcgcc
     6061 ggcgcgctca tcgtcggatc gttctcgtgg gcgttcggat ccctt
//
LOCUS       DASWAV010000452         2978 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U2665
            k141_63927, whole genome shotgun sequence.
ACCESSION   DASWAV010000452 DASWAV010000000
VERSION     DASWAV010000452.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960316
            Sequence Read Archive: SRR10260007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2978)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2978)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:47:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,929
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2978
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_63927"
                     /isolate="SMAG_U2665"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.36444 N 132.31889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(611..1786)
                     /locus_tag="VF958_09375"
     CDS             complement(611..1786)
                     /locus_tag="VF958_09375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007668732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS91 family transposase"
                     /protein_id="HEX9149357.1"
                     /translation="MIPPAHELAEVFRRHGARFRERMGERLPVACRRVMSAIEKCRTA
                     ALGGHMDRCTDCGHRAISYNSCRNRHCPKCQGGSQLEWLADREGELLPVPYFHVVFTI
                     PDALLSDIAFQNKRLVYGLLFKASAATLLTIGRDSQHLGADVGFFGVLHTWGQNLLHH
                     PHVHYVVPSGGLSRDRSRWISCRKDFFLPVRVLSSLFRRLFLEGIAEAFRNGALRLEG
                     ALAPLADPRAFAERLAQARAIDWVVYVNAFGAPRNVLRYLSRYVQRVALSNRRILAVE
                     EDVVRFLWRDSRHRNRQSVMSLPAHEFIRRFLLHVLPRGFRKVRHFGFLANRNRTKNI
                     ALCRQRICQSPDVRELPLPAEAISAATAKPRCTACGSTSLVRTDLSSCRMEARLDSS"
     gene            complement(1814..2683)
                     /locus_tag="VF958_09380"
     CDS             complement(1814..2683)
                     /locus_tag="VF958_09380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014857771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HEX9149358.1"
                     /translation="MTPLRQRMIDDMRIRNLSPRTREIYARQVAAFARHFQRPPEELG
                     AEQIRAYQLHLLDTRMSVSVLHQTVAALRFLYNVTLGKGFRVELIPYPRVARRLPEIL
                     SPEEVARFLHAVSRPKHHAALSLIYATGLRISEALGLTCADVDSRRMLIRVRQGKGMK
                     DRYVVLSPQLLKLLREYWRNEERPKTPASSWLFSSDGDPEKPLSQRTIRKAVGSAARK
                     AGLTKRVTPHVLRHCFATHLLEAGDGLRTIQVLLGHARISSTTIYTHVADPTLLKTNN
                     LLERLPSVPSLRR"
BASE COUNT          554 a          910 c         1051 g          463 t
ORIGIN      
        1 gccgctcgca gcgccacagg atgtactggg accgattcca acgtctcctg gagcggtatc
       61 cgcttcccga gccggtggga tacaccgtgc gtcctcgtcc gcagcgaaac cgttcctcga
      121 agagccggat gcggtagtcc cgcacgtccg gatctgtggg ggaggtggcc tcaaggcgcc
      181 aaggtcgaga tgttgtggca ccgccgggaa accaggcggc aaacggagaa gacgaaggtc
      241 gacctacagc gccaagggcc acctctaccc gaccggcacg cggagcgaag cggagcagaa
      301 aagagagtga gatccggggc cggtaagctg tgagcgttct ttcggaatcc tcgcagacgg
      361 ccgggtgccg gcgcgggagt cccgcgcgag gattcggaaa gaacgagttg aactgaaccg
      421 cgggtcgcgt gctatggcgt ttctgaagcg gcgtgctctt cgagcgtccc tgctcgcgag
      481 gtgagtgcca tcagcgacca gggatcgaag gccgggagtc cgcgtcgcag ttgacagctc
      541 ccgcgatgac gccatcgtgc ggcgccgggc gatgagacga gttgcggccg gcgataggga
      601 ggatgagcgt tcatgaggag tcgagcctcg cttccattcg acacgaagag aggtccgtgc
      661 gtacgaggct cgtcgagcca caggccgtgc agcgcggctt cgccgtcgcc gccgaaatgg
      721 cctcggctgg gagcggaagc tccctgacgt ccgggctctg gcagatgcgc tggcggcaca
      781 gcgcgatgtt cttcgtgcgg tttcgattcg ccagaaagcc gaagtgccgg actttgcgaa
      841 agccccgagg caacacatgg aggaggaagc gccggatgaa ttcgtgggcc ggcagcgaca
      901 tcaccgactg ccggttgcgg tgacgggagt ctctccagag aaagcggacg acatcctcct
      961 cgacggccag gatgcgcctg ttgctcagag caacgcgctg cacgtaacgg gagaggtacc
     1021 gcaggacgtt ccgaggagcg ccgaaggcgt tgacgtagac gacccagtcg atggcgcgcg
     1081 cctgagcgag ccgctcggca aaggctctcg gatcggcgag tggagccagc gctccttcga
     1141 gccggagcgc tccattccgg aatgcctcgg cgatcccttc gagaaacagg cgccgaaaca
     1201 ggctcgacag aacacgcacc ggcaggaaga agtccttcct gcacgagatc cagcgcgagc
     1261 ggtcgcgaga cagaccgccc gaagggacta cgtagtggac gtgcgggtgg tgaaggaggt
     1321 tctgccccca ggtgtgcagg acgccgaaga aacccacgtc ggcgcccaag tgctgggagt
     1381 ccctcccgat cgtcagcagc gtcgcggcgc tcgccttgaa gagcagcccg tagacgaggc
     1441 gcttgttctg gaaggcgatg tccgaaagga gggcgtctgg aatggtgaag acgacgtgga
     1501 aataggggac cggcaggagc tcaccttccc tgtcggcgag ccactcgagc tgcgagccgc
     1561 cctggcactt ggggcaatgc cggttgcggc acgagttgta ggagatcgct cgatggccgc
     1621 agtcggtgca gcggtccatg tgacccccga gcgccgccgt gcggcacttc tcgatggccg
     1681 acatgacccg gcgacaggct accggcaggc gctctcccat gcgctcgcga aagcgagccc
     1741 cgtggcgacg gaacacctcc gccagctcgt gggcgggagg aatcacggaa gcggtgaagc
     1801 ggagcgggcg ctcttaccgg cggaggctgg gcacggacgg cagacgctcc agaagattgt
     1861 tcgtcttcag gagagtcggg tccgccacgt gggtgtagat cgtcgtcgag gagatccgcg
     1921 cgtgccccag aagcacctgg atcgtccgca ggccatcgcc cgcttccaga aggtgcgtcg
     1981 cgaagcagtg gcgcagcacg tgaggggtga ctcgcttggt gagccccgcc tttcgtgccg
     2041 cgctcccgac cgccttgcgt atcgtgcgct gcgacagagg cttctcggga tccccgtcgc
     2101 tcgagaagag ccacgacgag gccggagtct tcggacgctc ctcgttccgc cagtactcgc
     2161 gcaggagctt gagcagctgc ggcgagagca ccacgtagcg atccttcatc cccttgccct
     2221 gcctcacgcg gatcagcatg cgtcggctgt cgacgtccgc gcacgtgaga ccgagcgctt
     2281 ccgagatccg caggccggtt gcgtagatca gcgagagcgc cgcgtggtgc ttcggacgag
     2341 agaccgcgtg caggaagcgg gcgacctcct cggggctgag gatctcggga agccgacggg
     2401 cgacgcgtgg gtacgggatc agctcgaccc ggaatccctt cccgagcgtg acgttgtaga
     2461 ggaaacgcag tgccgccacc gtctggtgca ggacgctcac cgacatccgc gtgtccagaa
     2521 ggtgaagctg gtacgcccgg atctgctccg ctccaagctc ctcgggcggt cgctggaagt
     2581 gccgcgcgaa ggcggctacc tgccgcgcgt agatctcccg cgtacgcggg gagaggtttc
     2641 ggatccgcat gtcgtcgatc attcgttggc gcaagggcgt catgacgaga gccctccttt
     2701 cgcgccaacg atgcagcccc ctgaaaaccc ctcgcgcacc aggggcaacc tcacccgacg
     2761 ccctaccgcg tcagcggttt agttcaatat ggggtgaggc gcaagtcggg atttcgaccc
     2821 ctgaaggggg agtccgggag ggacaggtat cagattcgag ctgacgtcgg ggggtcgaaa
     2881 gctgtgagcg ttctttcgga atcctcgcag acggccgggt gccggcgcgg gagtcccgcg
     2941 cgaggattcg gaaagaacga gttgaactga accgcggg
//
LOCUS       DASWAV010000453         3054 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U2665
            k141_146894, whole genome shotgun sequence.
ACCESSION   DASWAV010000453 DASWAV010000000
VERSION     DASWAV010000453.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960316
            Sequence Read Archive: SRR10260007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 3054)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3054)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:47:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,929
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3054
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_146894"
                     /isolate="SMAG_U2665"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.36444 N 132.31889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..379
                     /locus_tag="VF958_09385"
     CDS             <1..379
                     /locus_tag="VF958_09385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9149359.1"
                     /translation="FPPFATDPLSPDGRWFIGASSPGKFARYPVGGGEPRSINGIESG
                     EVPIRWSSDGRTIFTRRRVAPEPAKIWRLDPDNGRRAFVKEIRAAESGGVLFSLVLTA
                     DGRAHAYVLQRAHSQLYLAEGLK"
     gene            474..1877
                     /locus_tag="VF958_09390"
     CDS             474..1877
                     /locus_tag="VF958_09390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014076152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M24 family metallopeptidase"
                     /protein_id="HEX9149360.1"
                     /translation="MKISRRSLLATTGVAAAGSLIASKSASGAQQSGIDGSKSAALFL
                     TGDDGLKPATADRLPLEWHQGRVKLLQDYLQDQGYAGALLSDRWNIIYFTGLFHTSTE
                     RPFMVFLPADRMTTIWFHPGLDRDLVKSWWATETESYFDLQHAEGAFPNLGTVQQGEK
                     VNLFEWALKGLKKRGYDGKRIATDRELGSRQRDSVSKVFRKPTEFNSIADQCEKMRMV
                     KTPEELALWRRAYRVFDETHAFARDLLLAKGTDLTDFELGSAVQEFGTNLLMKGIKRD
                     GAPHTAVGIDLGVGVRTGRGTAYPHPNQFHHNRIQKGQALQIAGGVEIGGCGGELYRA
                     FLLAPWTDHQKKVWTVSRDSCLMQKDLSREGVACSTVAYQIHKFQVKEGMQKYIYHRP
                     AHGMGMEGHQPPYLALGDYTMLEPGMVFSVEPGLYDPENGFGVNFSDGFVVQSGDKPS
                     LQMSRLPWSEDWCLVRL"
     gene            1996..2784
                     /locus_tag="VF958_09395"
     CDS             1996..2784
                     /locus_tag="VF958_09395"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HEX9149361.1"
                     /translation="MDERKPAGAERRDERSELGRLQHLLERRTEALSSAQEALDLRDE
                     FLAIVSHDLRNPLNAIALNTQLLERLVSSSDPRLARISQSLNSSIAQMQRIISDLLDV
                     AAIEAGKLSVRMEPGDVRRSIEEAVESFRPIGEEKSIVLEAVTGPEPLRARFDPGRIG
                     QVLENLIHNALKFTPAGGRISVEGRMAGPSVEIRVQDTGPGMAPEEISVIFERFRQVE
                     KRGRRALGLGLYISRSIVESHGGRIRAESGPGKGSVFSFTLSPD"
     gene            complement(2765..>3054)
                     /locus_tag="VF958_09400"
     CDS             complement(2765..>3054)
                     /locus_tag="VF958_09400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012525091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX9149362.1"
                     /translation="LDYLDRSGAPCLVLLDLEMPVMNGWDFREKQLEDESLSGIPVVV
                     VTASDKGLSQRFPGAAGFLWKPLKFENVVAVLDRMCLRKKDVPPATSPATA"
BASE COUNT          555 a          972 c          995 g          532 t
ORIGIN      
        1 tttcccgccg tttgcaaccg atccgctctc gccggacggg cgctggttca tcggggcgtc
       61 gtcacccgga aaattcgcac gctaccccgt cggaggagga gagccgcgct cgatcaacgg
      121 catcgaatcc ggagaggttc cgattcgatg gagctccgac ggccgcacga ttttcacccg
      181 ccgacgggtc gcgccggagc ccgcaaagat ctggcgtctc gatcccgata acggccggcg
      241 ggcgttcgtg aaggagatcc gggcagcgga atccggaggg gttctcttca gtctcgtgtt
      301 gaccgccgac ggccgagccc atgcgtacgt cttgcagcgg gctcactcgc agctttatct
      361 cgcggaaggt ctgaagtgat gaggatccct gactcgcgct catcgccggc tgaccgcacg
      421 cgccgggcaa tctcactccg atacgattcg tccgctcttc gacggaggac accatgaaga
      481 tcagtcggcg ttcgcttctc gcgacgacgg gggtcgccgc ggcgggctcg ctcatcgcct
      541 cgaagtcggc gagcggggca caacagtcgg ggatcgacgg ctcgaaatcc gccgctctct
      601 ttctgaccgg ggacgacggg ctgaagccgg cgaccgctga ccgactgccg ctcgagtggc
      661 accaggggcg ggtcaagctc ctgcaggatt accttcagga ccagggctac gccggcgccc
      721 tgctctccga tcgctggaac atcatctact tcacgggcct cttccacacc tcgacggagc
      781 ggccgttcat ggtcttcctg cccgcagaca ggatgacgac gatctggttc cacccggggc
      841 tcgaccgcga tctcgtcaag agctggtggg cgaccgagac cgagtcgtac ttcgacctcc
      901 agcacgccga gggcgcgttc ccgaatctgg gaacggtgca gcagggcgag aaggtcaacc
      961 tcttcgagtg ggcgttgaag ggattgaaaa agcgcggcta cgacggaaag cggatcgcga
     1021 ccgaccgcga gctcggaagc cgccagcgcg actccgtgtc gaaagtcttt cggaagccga
     1081 ccgagttcaa ctcgatcgcg gaccagtgcg aaaaaatgcg gatggtcaag acgcccgagg
     1141 agctcgccct ctggcgccgt gcataccgtg tcttcgacga gacgcacgcg ttcgcgcgcg
     1201 acctcctgct cgcgaagggg accgacctca ccgacttcga gctcggctcc gccgtgcagg
     1261 agttcggcac gaatctgctt atgaagggaa tcaagcgtga cggtgcgcct cacacggccg
     1321 tcgggatcga cctcggcgtc ggcgtccgca cgggccgcgg gacggcgtat ccgcacccca
     1381 accagttcca tcacaacagg atccagaaag gccaggctct ccagatcgcc ggcggcgtcg
     1441 aaatcggggg ctgcggcgga gagctctacc gcgcatttct gctcgcgccg tggacggacc
     1501 accagaagaa ggtctggacg gtttcgcgcg actcatgcct catgcagaag gacctctcgc
     1561 gcgaaggcgt cgcgtgctcg accgtcgcgt accagatcca caagttccag gtgaaagagg
     1621 ggatgcagaa gtacatctac catcgtcccg cccacgggat gggcatggaa gggcaccagc
     1681 cgccgtatct cgcgctcggt gattacacga tgctggagcc cgggatggtt ttctcggtcg
     1741 aaccgggtct ctacgacccc gagaacgggt tcggcgtgaa cttcagcgac ggcttcgtcg
     1801 ttcagtcggg ggacaagccc tctctccaga tgtcgcgcct tccgtggtcc gaggactggt
     1861 gtctggtaag gctttaaacc aagtcgcggc gtggtttgag ttacgctttt ccttgaatgg
     1921 attcacgacc aacgattccg gggaagagtg gcgggactgc gggtcggcga gcgggattcc
     1981 ggcgaggagc cgaccgtgga tgaacggaaa cccgccggtg cggagcgccg tgacgagcgt
     2041 tccgagctgg gacggctcca gcaccttctc gaacggcgaa cggaggcgct ttcgagcgcg
     2101 caggaggccc tcgacctccg agacgagttc ctcgcgatcg tcagccacga cctgcggaac
     2161 ccgctgaacg cgatcgcttt gaacactcag cttctcgaga ggctcgtctc gtcgagcgac
     2221 cctcgcctcg ccaggatctc gcagagcctc aattcgtcga tcgcccagat gcagcggatc
     2281 atttcggacc ttctcgacgt cgctgcgatc gaggcgggga aactgtcggt ccggatggag
     2341 cccggcgacg tgcgaagatc aatcgaagag gcggtggaat cctttcgtcc gatcggcgag
     2401 gagaaatcga tcgtcctcga ggcggtcacc gggcccgaac cgctccgggc gcgcttcgac
     2461 cccggccgca tcggccaggt cctcgagaat ctgattcaca acgcgttgaa attcactccg
     2521 gcgggaggcc gtatttccgt ggaaggtcgg atggcgggcc cgagcgtcga aatccgggtc
     2581 caggacacgg ggcccggcat ggcgccggag gagatctcgg tgatcttcga gcgcttccgg
     2641 caggtcgaaa agcggggacg gcgagcgctc ggactcggcc tctacatctc ccgttccatc
     2701 gtcgagtcgc acggcggccg gatccgggcc gaaagcggtc cggggaaagg gagtgtcttc
     2761 tcctttacgc tgtcgccgga ctagtcgccg gcggtacgtc tttcttccgg aggcacatgc
     2821 ggtcgaggac cgcgacgacg ttttcgaact tcagcggttt ccagaggaac cccgcggcgc
     2881 cgggaaagcg ttggctcaag cctttgtcgc tcgccgttac gacgacgacc ggaattcccg
     2941 acaggctctc gtcctcgagc tgtttctccc ggaagtccca cccgttcatc accggcattt
     3001 cgagatcgag cagcacgagg cacggcgctc ccgaccgatc gaggtagtcg agag
//
LOCUS       DASWAV010000454        13296 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U2665
            k141_426495, whole genome shotgun sequence.
ACCESSION   DASWAV010000454 DASWAV010000000
VERSION     DASWAV010000454.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960316
            Sequence Read Archive: SRR10260007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 13296)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13296)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:47:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,929
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13296
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_426495"
                     /isolate="SMAG_U2665"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.36444 N 132.31889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..766)
                     /gene="hflX"
                     /locus_tag="VF958_09405"
     CDS             complement(<1..766)
                     /gene="hflX"
                     /locus_tag="VF958_09405"
                     /inference="COORDINATES: protein motif:HMM:TIGR03156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HEX9149363.1"
                     /translation="MDELERLVESAGGEVVARVVKERASPDPATYVGRGAVEEIGAIA
                     GDERAGLVVFDDELTPAQVRNLEERWGEDVTALDRPGLILDVFASHARTREARTQVSL
                     AQLQYLLPRLAGRYGHLSRLAGGIGGRGAGEQKLELDRRKIRDRIARLRHDLEKIETS
                     RKVRRRSRAGRFQVAIAGYTNAGKTTIFNRLTREAAYAADRLFATLDSRTAHGASSKL
                     SQVVFVDTVGFVRKLPPSLVASFRSTLSEIRDADLVL"
     gene            970..1875
                     /locus_tag="VF958_09410"
     CDS             970..1875
                     /locus_tag="VF958_09410"
                     /inference="COORDINATES: protein motif:HMM:TIGR02757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02757 family protein"
                     /protein_id="HEX9149364.1"
                     /translation="MSNLSPRELALADRLNRLSRSYAAAIDQRDPIRFAASCERSEDR
                     EVVAWIASAFAYGRVETILASVGRLVATLGSRPADALDRITDWRRFAREELAGFRHRF
                     HSGEDAAALLYAIARVRGEAGSLRPFFERELRAEDRDVEGLLSRAVGRILALDWRPVA
                     GRRTLPADSPVRFFFPSPASGSACKRWNLYLRWMVRRDAMDFGLWSSIPANRLVIPTD
                     THVHRVARRLGLTHRKAADWKTAREITDHLARFDPEDPVRYDYALCELGTIGICRPSL
                     AASVCNDCALAEACPAGRRRIRRAA"
     gene            1872..2408
                     /locus_tag="VF958_09415"
     CDS             1872..2408
                     /locus_tag="VF958_09415"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HEX9149365.1"
                     /translation="MKVRTILTIAMAVALAVVGTIWIRSLVPPESPTGSLAFLSAAPE
                     LPIYDRSGKKIDLADAKGRYLIVHFWATWCPPCVEEIPALSRFWEKYGNRADLVLYAV
                     SVDKDWKIIDNFATKNPNKIPIYRDPDAKTAARFGSSQYPETYIVNPAGRVLYRVQGA
                     MSWDDPEVRRKIEQLLAS"
     gene            complement(2585..5686)
                     /locus_tag="VF958_09420"
     CDS             complement(2585..5686)
                     /locus_tag="VF958_09420"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HEX9149366.1"
                     /translation="MPRRSPAEPIDHHYNIPRLNRVFAATGIVLTVVFIAMVVEDYSR
                     GWKKIQRAFGRIEAKKTREAALAARREVLGGDLQRLRGQLSQSNRELAAHRGELARLD
                     AKLKELAPKIYLADQQFKFTKASLDAQRYKYEKALSVAPSAAPNQKRGFDALNADLEE
                     KRVHLARLQKEETEVKAKRDALVERRDTAETSIEKLTADYRLARKHFASLQEDTIFKL
                     RNAAIIDMVNPSLRIQQVQLPDHLTDVNFMKIPRVDRCQTCHIAADRPGFDDAKLNAV
                     FRTHPRPHLMVSSESPHPVTLFGCSPCHGGRDRASSFWSAGHSPDDPHQDARWAKAFG
                     WKFDRFNETPILPMKYTEAGCYRCHSDETNFREAPTLDAGIKTIEVLGCWGCHRIEGL
                     ERQHIPKVGPSLEKVASKVTREWATRWVMKPDAFRASTRMPQFFYLENFVNVSGPRPP
                     TPAQKKMNEAGRVENDVMVNSIVAYLFDKSRHADVAPVPRTGDPARGQTLLSQRGCFG
                     CHVADPTARRDLVGTYRQFGPNLAGIGSKASRDWIYHWILDPKAWNPDTKMPNLRLTP
                     EDALDIAQYLSTLKAPPDFERTALPGTDSATLDRIAMYFEMADKTLIDAKAALAKMDL
                     HGKEVYAGEKLISHYGCFACHAIPGFENAKPIGTELTEEGSKAVHLLDFGFIHIPHTR
                     QDWFETKLTNPRIFDRDHARGWEERFRMPNFHLSRVERDRILTAVLGFQELNATESAR
                     KRLSPEEEAIERGRRIVKSHNCQGCHVIEGFGGSFRSLVADPSLAPPIIQGEGSKVQS
                     DWLFSFLKAPKTGQIRPWLEVHMPTFGFTAAELNDLTKYFASLDRASYPFLARDYVTD
                     PGRWAAGRKVFEMLKCRQCHPRNEQDMNAPGVDRASLAPNLQMAATRLRHDWISDWIR
                     RPDEWMPGTRMPTNFPKGDDGKRTSPIAALLDAPTFAADRAEFARLLGGDEAAKSFLN
                     DPDQVTKALRDYVWSVGINGGTAPAGASEAQPSTSGSEPAKATERAARPPAPRAASES
                     SGR"
     gene            complement(5691..6776)
                     /locus_tag="VF958_09425"
     CDS             complement(5691..6776)
                     /locus_tag="VF958_09425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010588316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9149367.1"
                     /translation="MAVVNALRGLVNFLSAPQYLVTVALVLLVIAINVPAIWSKKGGI
                     FLGIGMVLFFALSYLDPNFNAVATLPDNVPIVGMIFLVGFFFWWAMHNAYENDRRIAE
                     GRPTIEGEDSAQKVFSWPDLVYVELICLVVVMAVMIVWSIVLKAPLEEPANPTDSPNP
                     SKAPWYFLGLQEILVYYDPWMAGVVLPSLIIVGLMAIPYIDTNPKGNGYFTFRERKWE
                     ITIFLVGFLVLWCVLIVLGTFLRGPNWNFFGPYETWDVNKLVPLNNINLSEIVWIKIL
                     KTAMPSFWLFREFVGLGLVALYMGVLPPILSKTVLRRFYEKMGTVRYLIFVNLALIML
                     ALPIKMYLRWIFNLKYIVNIPEISFNI"
     gene            complement(6796..7584)
                     /locus_tag="VF958_09430"
     CDS             complement(6796..7584)
                     /locus_tag="VF958_09430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018049746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b N-terminal domain-containing
                     protein"
                     /protein_id="HEX9149368.1"
                     /translation="MGRRWNRFRESVVESQFWKSVFRVGIPNTNRKRVLAMLGNVVLH
                     LHPVKTRKSGVKMRYTWCAGGVTFFLFLVLTFTGLLLMFYYRPTVEYAFVDILDLREQ
                     VPFGIMREVHRWGAHAMVISVWIHMFRVFMTGSYKPPREFNWVIGVILLVLTLLLSFT
                     GYLLPWDQLAIWAITVGSNMAAATPLIGIEGPGAGLLKIGDIQLVTSSNDARFALLGG
                     RFVGAGALLRFYVLHCVGIPLVAAFLMAVHFWRVRKDGGISGPV"
     gene            complement(7630..8115)
                     /locus_tag="VF958_09435"
     CDS             complement(7630..8115)
                     /locus_tag="VF958_09435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014242928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinol-cytochrome c reductase iron-sulfur
                     subunit"
                     /protein_id="HEX9149369.1"
                     /translation="MTRRSTLKWMTIAWVGFAAATGAGLTATLRFLFPNVLFEPPTRF
                     KAGDPSTYSFGVDERWKGKYRVWIVRNTDSVYALVARCTHLGCTPNWLAAQNKFKCPC
                     HGSGYYMSGVNFEGPTPRPLERAEIGISADDGQLVVDTATRFLYERGQWSDPKSFVKM
                     V"
     gene            complement(8227..9147)
                     /locus_tag="VF958_09440"
     CDS             complement(8227..9147)
                     /locus_tag="VF958_09440"
                     /inference="COORDINATES: protein motif:HMM:NF025030.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HEX9149370.1"
                     /translation="MTDGPPDGPDSEEELPEDEEVSPRTPAARLVIRFFVLPLLVVGT
                     AVGIFLVFSLMTFERRSPRDYLAEVRGGSAGRRWQAAFELSRRIGTMKPGPERDAIAT
                     ESLRLFRTLSPARSEDVRVRRYLVLVLGKLRDRSAVPDLVGAAADPDPETRLYAIWAL
                     GMIGDRRGVDAVLEASRSEDPGMRKMAAYVMGKLGEERAVDRLKVLLEDRVPDVRWNS
                     AIALASLGDGSGIVVLRSMIDREALGRQAQLTSDQAETAMVSALKAFALLRDGGSLTL
                     LEKVAREDPNLRVRDAARRAVEATRGRAAA"
     gene            complement(9144..9590)
                     /locus_tag="VF958_09445"
     CDS             complement(9144..9590)
                     /locus_tag="VF958_09445"
                     /inference="COORDINATES: protein motif:HMM:NF024247.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HEX9149371.1"
                     /translation="MPHFITDTCIGCGVCEIKCPTHTITGDKSEKYVIHADRCIDCSV
                     CARYCPVSCIQDQKGELVEKVKPRDIPKAIVDRDLCTGCEFCIDICPFDCIYLIDDPL
                     QETFHKIAEVEAGPCVSCRLCETVCDKDAIFVPNPITQAKRYLSVL"
     gene            9705..10262
                     /locus_tag="VF958_09450"
     CDS             9705..10262
                     /locus_tag="VF958_09450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9149372.1"
                     /translation="MKELRAVVFGLVIVILVAAALAFWRRPAPEFARVKGFKVEVKER
                     EGDSTRRVTFSVPANLVARVAKLSPIRRFGGDIRTDWDGDITPREILDAAAGSAPGKP
                     GVIKRGNNTIEVLAEGTALAIDIKDDWGKSVHLRVPRAIVESFSGDREITPREILKKI
                     DELGPGDVVVVRDGDKEVTITAEPR"
     gene            10313..12358
                     /locus_tag="VF958_09455"
     CDS             10313..12358
                     /locus_tag="VF958_09455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012870618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HEX9149373.1"
                     /translation="MANRLAREKSPYLLQHAENPVDWYPWGDEAFGRARTENKPIFLS
                     IGYSTCHWCHVMERESFENEAIAELLNRNFVPIKVDREERPDVDDVYMTAVQTMTGSG
                     GWPLSLFLTPAGRPFYGGTYFPPDDRFGRPGFPALLRAISDAWKTRRDELEDSAGRLV
                     EHLDLAARGPASGREVGGASLDNAARALAAEFDPRNGGFGGAPKFPPAMRLEFLVRHF
                     LRTGEPKAREMVETTLLRMAAGGLYDHVGGGFHRYSTDAKWLVPHFEKMLYDNALLAR
                     AYLLAYRAFGNTDFARVARETLDYLLAEMTPGEGGFFVAQDADSAGEEGTFYVWNPES
                     LEAAVGNDVAPIVAARYGVTEAGNFENGQTVLSIVTSVAGLAAQFSVPDGRVEEILRA
                     ARGQMYEARSRREPPLTDKKLLTDWSALAISAFALAARLLSEPRYEAAARRAADRILA
                     RCRVEGDLFHREKDGEAEISGFASDYAFFVEALLDLYEATFEPRYFRAAVELQNEMEE
                     RFTDPRGGYFLAAERHDRLIVRPRELFDGATPSSNSVAAMNLLRLSTFTGQPKYRDRA
                     ESIFSAFAEYLERAPAAFPRLLCALDYRQDEPREVVLSGEAGRPDFEALRAAAFGSRR
                     LNRVVAHADAAESLSDVAPLVASRGSSDGAAAAWVCRNFACLRPTSDPAELAAALDV"
     gene            12351..12749
                     /locus_tag="VF958_09460"
     CDS             12351..12749
                     /locus_tag="VF958_09460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9149374.1"
                     /translation="MSDPVHPSFRVVGPLEASAAPRRRGPGIAVVGVAIYALLFLGAL
                     WYFKREPTVPRPKAGVVARPPTRLSLLAGEGLAPAARGKYLARIGADHCDCGCELSLA
                     QCLARDRSCVRSPALASERLRAAAAPAPAP"
     gene            12746..>13296
                     /locus_tag="VF958_09465"
     CDS             12746..>13296
                     /locus_tag="VF958_09465"
                     /inference="COORDINATES: protein motif:HMM:NF013046.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histone deacetylase"
                     /protein_id="HEX9149375.1"
                     /translation="MTTGLVYDDAFLLHRAPYEHPENPGRLAAIWARLNEEGIAGRCR
                     RVSAREATREELRAVHTLEHIDRIAATADPSFAQLDPDTYTSRHSAAAARLAAGGLID
                     LAGKVLSGDVSNGLALLRPPGHHAEADRAMGFCLFNNVAVATKAAQRAGVERVAIVDW
                     DLHHGNGTQHTFENDPNVLYFST"
BASE COUNT         2163 a         4408 c         4510 g         2215 t
ORIGIN      
        1 gcagcacgag gtcggcgtcg cgaatctccg acagcgtcga tcgaaatgac gcgacgagcg
       61 acggcggcag cttgcgcacg aacccgaccg tgtcgacgaa aacgacctgc gagagtttcg
      121 aggaggcacc gtgcgcagtc cgagaatcga gggtcgcgaa cagacggtcg gccgcgtatg
      181 ccgcctcgcg cgtcagccgg ttgaagatcg tcgtcttgcc ggcgttggtg tagccggcga
      241 tcgcgacctg gaagcgcccg gcgcgcgagc ggcggcgcac cttgcgggac gtctcgatct
      301 tctcgaggtc gtgtcgcagc cgcgcgatgc ggtcgcggat cttgcgccgg tcgagctcga
      361 gcttctgttc gcctgcgccg cgcccgccga ttccgcccgc gagccgcgag aggtggccgt
      421 acctcccggc cagtctcggc aggagatatt ggagctgtgc gaggctgacc tgcgttcgcg
      481 cctcccgcgt gcgggcgtgg gaagcgaaca cgtcgaggat caagccgggt cggtcgagcg
      541 ccgtcacgtc ctcgccccag cgctcttcga ggttcctcac ctgggcggga gtgagctcgt
      601 cgtcgaagac gaccaggccg gcgcgttcgt cgccggcgat cgcgccgatc tcctcgactg
      661 cgccgcgccc cacgtacgtg gcgggatccg gagatgcgcg ctctttcacg acccgggcga
      721 cgacttctcc cccggcggac tcgacgagcc gctcgagttc gtccaagtgc tcctcggaca
      781 cggagcgcgg cactgcccgc gaaaacacgc ccacgatgac gacccgccgg gaagacgtga
      841 ccgggccgcg agaacgtctc agatgggagc gcgtcaaatc agtcgagtgt cgaggagtgc
      901 aacgcgacgc gaggtcgcgg cgtcgaggcc ggcagtcacc gttgccgatg gtaagatcca
      961 ccgcgcacag tgtcaaatct ctccccccgg gagcttgcgc tcgccgatcg cctcaaccgc
     1021 ctttcgcgct cctacgccgc cgccatcgat cagcgcgacc cgatccgctt cgcggcgtct
     1081 tgcgagcgat cggaggaccg ggaggtcgtg gcgtggatcg cctccgcgtt cgcctacgga
     1141 cgggtggaga ccatcctcgc ctcggtcggg cgtctcgtcg cgacgctcgg ttccagaccc
     1201 gcggacgcgc tcgaccgcat caccgactgg cgccggttcg cgcgggagga gctcgccggt
     1261 ttccggcacc gatttcactc gggagaggac gccgccgcgc tcctgtacgc gatcgctcgt
     1321 gtgcgcggag aggccggctc gctccgtccg ttcttcgagc gggaactgcg cgcggaggac
     1381 cgggacgtcg aagggcttct ttcgcgcgcc gtcgggcgga tcctcgcgct cgactggcgg
     1441 ccggtcgccg ggcggcggac ccttccggcg gattcgccgg tgcgtttctt ctttcccagc
     1501 ccggcctccg gctccgcgtg caagcggtgg aacctctacc tgcgatggat ggtccgacgg
     1561 gacgccatgg acttcggcct gtggagctcg atcccggcga accgcctcgt gattccgacc
     1621 gacacacacg tgcaccgcgt cgcgcggagg ctcggtctga cgcatcgcaa ggccgcggac
     1681 tggaagaccg cgcgtgagat cacggaccac ctcgcacgat tcgacccgga ggaccccgtg
     1741 cgatacgact acgcgctttg cgaactcggg acgatcggga tctgccgccc gtccctcgcc
     1801 gcgtccgtgt gcaacgactg cgcgctcgcc gaagcctgtc ccgccgggcg gcggcggatc
     1861 cggagagccg catgaaagtc cgcacgatcc tgaccatcgc gatggcggtg gcgctcgccg
     1921 tcgtcgggac gatctggatc cgctcgctcg tgccgcccga gtcgccgacg ggttcccttg
     1981 cttttctctc cgcggctccg gagctgccga tctatgaccg ttcgggcaag aagatcgatc
     2041 tggcggacgc gaaggggagg tacttgatcg tccacttctg ggcgacgtgg tgtcctccct
     2101 gcgtcgaaga aatcccggct ctctctcgtt tctgggagaa gtacggcaac cgcgccgacc
     2161 tggtgctcta cgccgtctcg gtggacaaag actggaagat catcgacaac ttcgccacga
     2221 agaacccgaa caaaatcccg atctaccgcg acccggacgc gaaaacggcc gcccgcttcg
     2281 gatcgagcca gtatcccgag acgtacatcg tcaacccggc cggccgcgtt ctgtatcgcg
     2341 tgcagggcgc gatgtcgtgg gacgaccccg aagtccgccg gaagatcgag cagttgctgg
     2401 cgtcctgacc aagtcgcggc gacgcggtca tccgagccga gacgccggcg agcgtagccc
     2461 gtctactccc ggttgcgctt ctcgcgcatg agccagagaa agagcacgac cgcggcggcg
     2521 gcgagtccga ggctccccca gtagaggacg agatcgaggt cgagagccgg caagccggcc
     2581 gagtctagcg tcccgagctc tccgacgccg cgcgcggggc cggcggacgc gccgcccgct
     2641 cggtcgcctt cgccggctcg gagccggacg tcgacggctg cgcttcgctc gcgccggcag
     2701 gcgccgtccc gccattgatg ccgaccgacc agacgtagtc ccgcagcgcc ttcgtcacct
     2761 ggtcgggatc gttcaagaac gacttcgcgg cctcgtcgcc ccccagcagc cgggcgaact
     2821 ccgcccggtc cgcggcgaaa gtcggtgcgt cgagaagcgc cgcgatcggc gacgtgcgct
     2881 tcccgtcgtc ccccttcggg aagttcgtcg gcatgcgggt gcccggcatc cactcgtcgg
     2941 gtcgccggat ccagtcggag atccagtcgt gccgcagccg ggtcgcggcc atctggaggt
     3001 tcggcgcgag cgacgcgcgg tcgactccgg gagcgttcat gtcctgctcg ttcctcgggt
     3061 ggcactgcct gcacttcaac atttcgaaga ccttcctgcc ggcggcccac cggccggggt
     3121 ccgtcacgta atcgcgcgcg aggaacggat acgacgcgcg gtcgagcgac gcgaagtact
     3181 tcgtcagatc gttcagctcc gcggccgtaa aaccgaacgt cggcatgtga acttcgagcc
     3241 acggccggat ctgaccggtc ttcggcgcct tcaggaagga gaagagccag tcgctctgca
     3301 ctttcgagcc ctcgccctgg atgatcggcg gcgccaggga aggatcggcg acgagcgatc
     3361 ggaacgagcc gccgaatccc tcgatgacgt ggcagccctg gcagttgtgg ctcttgacga
     3421 tgcgccggcc gcgctcgatc gcctcttctt caggcgacag acgcttgcgt gcgctctcgg
     3481 tggcgttcaa ttcctggaac cccaggaccg cggtcagaat cctgtcgcgc tcgacccgcg
     3541 aaaggtggaa gttcggcatc cggaacctct cctcccagcc tcgtgcgtgg tcgcggtcga
     3601 aaatccgcgg gttcgtgagc ttcgtctcga accagtcctg gcgggtgtgc gggatgtgga
     3661 tgaaaccgaa gtcgaggagg tggacggcct tcgagccctc ctcggtgagc tcggtgccga
     3721 tcggcttcgc gttctcgaag ccggggatcg cgtgacacgc gaagcagccg tagtgcgaga
     3781 tcagcttctc gcccgcgtag acctccttgc cgtggagatc catcttcgcg agcgccgctt
     3841 tcgcgtcgat caacgtcttg tcggccatct cgaagtacat ggcgatgcga tcgagcgtcg
     3901 cggagtcggt ccccggaagc gccgtccgct cgaagtcggg aggcgccttc agtgtcgaga
     3961 ggtactgcgc gatgtcgagg gcgtcctccg gggtcaggcg caggttcggc atcttcgtgt
     4021 cggggttcca cgccttcgga tcgaggatcc agtggtagat ccagtcgcgg ctcgctttcg
     4081 agccgatgcc cgcgaggttc ggcccgaact ggcggtacgt gcccacgaga tcgcgccgag
     4141 cggtcggatc ggcgacgtgg catccgaaac agccacgctg cgagagcagc gtctgacccc
     4201 gcgccgggtc gccggtccgg ggcacgggcg ccacgtcggc gtggcgcgac ttgtcgaaca
     4261 ggtacgcgac gatcgagttg accatcacgt cgttctcgac ccgccccgcc tcgttcatct
     4321 tcttctgggc gggcgtggga ggccgagggc ccgacacgtt gacgaagttc tccaggtaga
     4381 agaactgagg catccgcgtc gatgcgcgga aggcatcggg tttcatcacc cagcgggtgg
     4441 cccactcgcg cgtgaccttc gacgcgacct tctcgagcga gggtccgacc ttcgggatgt
     4501 gctgcctctc gagcccctcg atccgatggc acccccagca gccgaggact tcgatcgtct
     4561 tgatgcccgc gtcgagcgtc ggggcctcgc ggaagttcgt ctcgtccgag tggcagcggt
     4621 aacagcccgc ctccgtgtac ttcatcggaa gaatcggcgt ctcgttgaag cggtcgaact
     4681 tccatccgaa cgccttcgcc cagcgagcat cctggtgtgg gtcgtcgggc gagtgcccgg
     4741 ccgaccagaa cgacgaggcg cggtcgcgcc cgccgtggca cgggctgcac ccgaagagcg
     4801 tgaccggatg cggcgattcg ctggacacca tgaggtgcgg gcgcgggtgc gttcggaaca
     4861 cggcgttcag cttggcgtcg tcgaacccgg gtcggtcggc ggcgatgtgg cacgtctggc
     4921 accggtcgac ccgcgggatc ttcatgaagt tgacgtcggt caggtggtcc gggagctgca
     4981 cctgctggat tcgcagcgac gggttgacca tgtcgatgat tgcggcgttg cgcagcttga
     5041 agatcgtgtc ttcctggagg ctcgcgaagt gcttgcgcgc cagccggtag tcggcggtca
     5101 gcttctcgat cgaggtctcg gccgtgtcgc ggcgctcgac gagcgcgtcg cgcttcgcct
     5161 tgacctcggt ctcctccttc tggagtcggg cgaggtgcac ccgtttctcc tcgaggtccg
     5221 cgttgagcgc gtcgaagccg cgcttctggt tcggggcggc gctcggcgcg accgagagcg
     5281 ctttctcgta cttgtagcgc tgcgcgtcca gtgacgcctt cgtgaacttg aactgctggt
     5341 cggcgaggta gatcttgggc gcgagttcct tcagcttcgc gtcgagacgc gcgagctccc
     5401 cgcggtgggc ggcgagctcc cggttcgact gcgagagctg tccccgcagg cgctgaaggt
     5461 cgccgccgag cacctcccgc cgcgcggcca gcgccgcttc gcgggtcttc ttcgcctcga
     5521 tgcggccgaa cgcgcgctgg atcttcttcc agccgcgcga gtagtcctcg acgaccatcg
     5581 cgatgaagac gaccgtcagc acgatcccgg tggccgcgaa gacgcggttc aggcggggaa
     5641 tgttgtagtg atgatcaatg ggctcggcgg gggatcggcg aggcatcgcg tcagatgttg
     5701 aagctgatct cggggatgtt gacgatgtac ttcaagttga agatccatcg caggtacatc
     5761 ttgatcggca gggccagcat gatgagcgcg aggttgacga agatgaggta ccgcaccgtc
     5821 cccatctttt cgtagaagcg gcgcaggacg gtcttcgaga gaatcggcgg gaggactccc
     5881 atgtagaggg ccaccagtcc gagcccgacg aactcccgga agagccagaa actcggcatc
     5941 gcggtcttca gaatcttgat ccagacgatc tccgacaggt tgatgttgtt cagggggacg
     6001 agcttgttga cgtcccacgt ttcgtacggt ccgaagaagt tccagttcgg acctcggagg
     6061 aaagtcccga gcacgatcag aacgcaccag agcaccagga atccgacgag gaagatcgtg
     6121 atctcccact tgcgctcgcg gaacgtgaag tagccgttcc ccttcgggtt cgtgtcgatg
     6181 tacgggatcg ccatgaggcc gacgatgatg agcgaaggca ggacgacgcc cgccatccag
     6241 gggtcgtagt agacgaggat ctcctgcagg ccgaggaagt accacggcgc cttggacggg
     6301 ttcggggaat cggtcgggtt cgcgggctct tcgagcggcg ccttcaggac gatcgaccag
     6361 acgatcatca ccgccatgac gaccacgagg caaatgagct ccacgtagac gaggtccggc
     6421 caggagaaga ccttctgggc cgaatcctcc ccttcgatcg tgggccttcc ctccgcgatg
     6481 cgtcggtcgt tctcgtaggc gttgtgcatc gcccaccaga agaagaagcc gacgagaaag
     6541 atcatcccga cgatcggcac gttgtcgggc agcgtcgcga cggcgttgaa gttcggatcg
     6601 aggtaggaga gcgcgaagaa gaggaccatg ccgatcccga ggaagattcc ccccttcttg
     6661 ctccagatcg ccgggacgtt gatcgcgatc acgagcagca cgagcgccac cgtgacgagg
     6721 tactggggcg ccgagaggaa gttgacgagg ccccggagag cgttgacgac ggccatcggt
     6781 ggggaggtct tccgactaca ccggaccgga gataccgccg tccttgcgca ctcgccagaa
     6841 gtggacggcc atcaggaacg cggcaacgag cgggattccg acgcagtgca ggacgtaaaa
     6901 gcgtaggagc gcgccggcgc cgacgaagcg tccgccgagg agtgcgaacc gcgcgtcgtt
     6961 ggacgacgtg acgagctgga tgtcgccgat cttgagcagg ccggcgcccg gaccctcgat
     7021 gccgatgagc ggagtcgcgg cggccatgtt cgagccgacc gtgatagccc aaatcgcgag
     7081 ctggtcccac ggcaggaggt agccggtgaa cgagaggagg agcgtgagca cgaggaggat
     7141 gacgccgatg acccagttga actcccgggg cggcttgtac gagccggtca tgaacacgcg
     7201 gaacatgtgg atccacacgg agatgaccat cgcgtgcgcg ccccaccggt ggacctcccg
     7261 catgattccg aacggaacct gctcgcgcag gtcgaggatg tcgacgaacg cgtactcgac
     7321 cgtgggccgg taatagaaca tgaggagcag ccccgtgaac gtgaggacaa ggaagaggaa
     7381 gaacgtcacg cccccggcgc accacgtgta gcgcatcttg acgccggact tgcgggtctt
     7441 gaccgggtgg aggtggagga cgacgttgcc gagcatcgcc agcacgcgct tgcggttcgt
     7501 gttggggatg ccgacccgga agacggactt ccagaactgg ctctcgacga cgctctctcg
     7561 aaatcggttc caccgacgac ccaatgcgac ctcctccggg actccttgac gaggggattg
     7621 aaaagagagc tacaccatct tcacgaaaga cttgggatcg ctccactggc cgcgttcata
     7681 cagaaaccgg gtcgccgtat cgacgacgag ctggccgtcg tcggccgaga ttccgatctc
     7741 ggcgcgctcg agcgggcgcg gggtcgggcc ttcgaagttc acgcccgaca tgtagtaccc
     7801 ggagccgtgg caggggcatt tgaacttgtt ctgcgccgcg agccagttcg gcgtgcatcc
     7861 caggtgcgtg cagcgcgcca cgagagcgta gaccgagtcg gtgttcctca cgatccagac
     7921 gcgatacttg cccttccatc gttcgtcgac gccgaaggag tacgtcgagg ggtcgcccgc
     7981 cttgaaccgc gtcggcggct cgaagaggac gttcgggaag aggaagcgca gggtcgccgt
     8041 caggccggcg ccggtcgcgg cggcgaaccc gacccaggcg atcgtcatcc acttgagtgt
     8101 cgagcgcctc gtcactccgt cctgcaggac gtccggagcc gccgatgccg acggctcgac
     8161 tacccttctc tgcggtgtcg acatgccccg cgcgagacta ccagaaccga aagggagcgc
     8221 tcaacatcag gctgcggcgc gcccgcgcgt cgcttcgacg gccctgcgcg cagcgtcgcg
     8281 aacgcgcagg ttcggatctt cgcgcgccac cttttcgaga agcgtgaggc tgcctccgtc
     8341 acgcaggaga gcgaaggctt tcagcgcgct caccatcgcc gtctcggcct gatcgctcgt
     8401 cagctgcgcc tggcggccga gcgcctcgcg gtcgatcatc gagcgcagga cgacgatgcc
     8461 ggaaccgtcg ccgagcgacg cgagcgcgat cgccgaattc catcggacgt cggggacgcg
     8521 gtcctccagc aacactttca accgatcgac ggcccgctcc tccccgagct tgcccatcac
     8581 gtaggccgcc atcttgcgca tccccggatc ttcggagcgc gaggcctcga ggacggcgtc
     8641 cacgccccgt cgatccccga tcatcccgag cgcccagatc gcgtagaggc gggtttcggg
     8701 atccggatcc gccgcggcgc cgacgaggtc gggcacggcg gagcggtccc ggagctttcc
     8761 gagcacgagc acgagatagc gccgcacccg gacgtcctcg gaccgcgcgg gcgacagcgt
     8821 gcggaagagc cgcaggctct cggtcgcgat cgcatcgcgc tccggacccg gcttcatcgt
     8881 gccgatccgg cgggaaagct cgaaggccgc ctgccagcgc cggccggcgc ttccgccacg
     8941 cacttcggcc aggtagtcgc gtggcgaccg ccgctcgaac gtcatgagcg agaagacgag
     9001 gaagatgccg accgcggtgc cgacgacgag gagcggcagg acgaagaacc ggatgacgag
     9061 gcgtgccgcc ggagtgcgcg gggagacctc ttcgtcttcg gggagctcct cctcggagtc
     9121 cggaccgtcg ggcggaccgt ctgtcacagg accgacaggt agcgcttcgc ctgcgtgatc
     9181 gggttcggga cgaagatcgc gtccttgtcg cagacggtct cgcagaggcg gcaggagacg
     9241 cacggtcccg cttccacctc ggcgatcttg tggaaggtct cctggagagg gtcgtcgatc
     9301 aggtaaatgc agtcgaacgg gcagatgtcg atgcagaact cacagccggt gcagaggtcc
     9361 cggtcgacga tcgccttggg gatgtcgcgc ggcttgacct tctcgacgag ctcgcccttc
     9421 tggtcctgga tgcacgacac ggggcaataa cgcgcacaga ccgagcagtc gatgcagcgg
     9481 tccgcgtgga tgacgtactt ctcgctcttg tcgccggtga tcgtgtgggt cgggcacttg
     9541 atctcgcaga ccccacagcc gatgcacgtg tcggtgatga aatgaggcat cgctctccga
     9601 aaacgctgaa aatagacccg ttattctgcg cgcccgaatg gggaaaaata ccacgggcgg
     9661 aacttttcgg ggccgctcgc gtttcgagag ggaggagagt ttccatgaag gagcttcgag
     9721 cggtcgtctt cggcctcgtg atcgtcatac tcgtggcggc cgcgctcgcg ttctggcgac
     9781 ggccggctcc ggagtttgct cgcgtgaaag gctttaaggt cgaggtcaag gagcgtgagg
     9841 gcgactcgac ccgtcgggtc acgttttccg tccccgccaa cctcgtggcg cgagtcgcca
     9901 agttgtcgcc gatccgccgt ttcgggggtg acatccgcac ggactgggat ggggacatca
     9961 cgccgcgcga aattctcgac gcggcggcgg ggagcgcccc cggtaagccg ggcgtcatca
    10021 agaggggcaa caacacgatc gaggtgctcg ccgagggcac cgcgctcgcc atcgacatca
    10081 aggacgattg gggaaagagc gtccacctgc gcgtaccgcg cgcgatcgtc gagagcttct
    10141 cgggcgatcg cgagatcacg ccccgggaga tcctgaagaa aatcgacgag ctggggccgg
    10201 gggacgtcgt cgtcgtgcgg gacggggaca aggaagtcac gatcaccgcc gagccccgtt
    10261 gagcccgttt cccgaaagga atcccgcccg tttgaggtag gttgtcccct ccgtggccaa
    10321 ccgtctcgcc agggaaaaga gtccctacct cctccagcac gccgagaacc cggtcgactg
    10381 gtatccctgg ggcgacgagg ccttcggccg cgcaaggacg gagaacaagc cgatcttcct
    10441 ctcgatcggc tactcgactt gccactggtg ccacgtcatg gagcgggagt cgttcgagaa
    10501 cgaggcgatc gcggagctcc tcaatcgcaa cttcgttcca ataaaggtcg accgcgagga
    10561 gcgccccgac gtcgacgacg tctacatgac ggccgtgcag acgatgacgg gcagcggcgg
    10621 ctggccgctc tccctcttcc tgacccccgc gggccgcccc ttctacggcg gtacgtattt
    10681 cccgccggac gaccggttcg gccggcccgg ctttcccgcg ctcctccggg cgatctccga
    10741 cgcctggaaa acgcggcgcg acgagctcga ggattcggcg ggacggctgg tcgagcacct
    10801 ggacctcgcg gctcgcgggc cggcctccgg ccgggaagtc ggcggagcct ccctcgacaa
    10861 cgccgcccgc gccctcgccg ccgagtttga tccgcggaac ggcggcttcg gtggcgcgcc
    10921 gaagttcccg cctgcgatgc gcctcgagtt cctcgtccgg cacttcctgc gcaccggcga
    10981 gccgaaggcg cgcgagatgg tcgagaccac tctcttgagg atggcggccg ggggcctcta
    11041 cgaccacgtc ggaggcggat ttcaccggta ctcgaccgac gcgaagtggc tcgtcccgca
    11101 cttcgagaag atgctgtacg acaacgcgct ccttgcgcgc gcatatctgc tcgcgtaccg
    11161 cgctttcggc aatacagact tcgcgagggt cgcgcgggag acgctcgact acctgctcgc
    11221 cgaaatgact cccggcgagg gggggttctt cgtcgcgcag gacgccgact cggccggcga
    11281 ggaggggacc ttttacgtct ggaacccgga gtcactcgag gccgccgtcg ggaacgacgt
    11341 cgcaccgatc gtcgcggcgc ggtacggcgt aacggaggcg ggcaacttcg agaacgggca
    11401 gaccgtgctt tcgatcgtga cgtcggtcgc cgggctcgcc gcgcagtttt cggtcccgga
    11461 cgggcgcgtc gaggaaatcc tccgcgcggc gcgcgggcag atgtacgagg cgcggtcgcg
    11521 ccgcgagccg ccgctcacgg acaagaagct cctgacggac tggagcgccc tcgcgatctc
    11581 ggcgttcgcg ctcgccgctc gcctgctctc cgaaccccgc tacgaggcag ccgcccgccg
    11641 cgcagcggac cggatcctgg cgcgctgccg ggtcgaaggg gacctctttc atcgcgagaa
    11701 agatggagag gcggagattt ccgggtttgc ctcggattac gctttcttcg tggaggcgct
    11761 gctggatctc tacgaggcga cgttcgagcc acggtatttt cgggccgcgg tcgagctgca
    11821 gaacgagatg gaggaacggt tcaccgaccc gcgcggtggt tattttctcg ctgcggagcg
    11881 gcacgatcgg ctcatcgtcc gtccgcgcga gctcttcgac ggcgccactc cgtcttcgaa
    11941 ttccgtcgcg gcgatgaacc tgctgcgcct ttcgacgttt accggccagc cgaagtaccg
    12001 tgatcgggcc gagtcgatct tttccgcgtt cgccgagtat ctcgagcgcg cgcccgccgc
    12061 cttccctcgg ctgctctgcg cactcgacta ccgacaggac gagccgcgtg aagtcgtcct
    12121 ctccggcgag gcgggccgcc cggacttcga ggcgctccgc gcggcggcgt tcggcagccg
    12181 gagactcaac cgcgtcgtgg cgcacgcgga cgccgccgag agtctctccg acgtcgcgcc
    12241 gctcgtggct tcgcgcggtt cttccgacgg cgcggctgcc gcgtgggtgt gccggaactt
    12301 cgcctgcctc cggccgacgt ccgacccggc ggagctcgcc gcggcgctcg atgtctgatc
    12361 cggttcaccc ttcgttccgg gtcgtcggtc ccctcgaggc gagcgccgcc ccccgacgtc
    12421 gcgggccggg gattgcggtg gtcggcgtcg cgatctatgc gctgcttttc ctgggcgctc
    12481 tctggtactt caagagagag ccaacggttc cgaggccgaa ggccggggtg gtcgccaggc
    12541 cgcccactcg cctgtcgctt ctcgccgggg aaggtctcgc accggccgcg cgcggcaaat
    12601 acctcgcccg aatcggcgcc gaccactgcg actgcggatg cgaactctcg ctcgcgcagt
    12661 gcctggcccg cgaccgctcg tgcgtccgca gccccgccct cgccagtgag cgtctgcgag
    12721 ccgccgccgc tccggcgccc gcgccgtgac gacgggcctc gtctacgacg acgcgttcct
    12781 cctccaccgg gctccctacg agcacccgga gaatcccggc cgtcttgccg cgatctgggc
    12841 gcgactcaac gaggagggga tcgccggccg ttgccgccgc gtgtccgccc gtgaagcgac
    12901 ccgcgaagag cttcgggccg tgcacacgct cgagcacatt gatcggatcg ccgcgaccgc
    12961 ggatcccagc ttcgcgcagc tcgatcccga tacgtacacg tcgcggcatt ccgcggccgc
    13021 agcacggctt gcagccggtg gcctgatcga tctcgccggg aaagtcctgt cgggcgacgt
    13081 ctcgaacggg ttggcgctgc tgcggccgcc cggacaccac gcggaggccg atcgcgcaat
    13141 gggcttctgt ctcttcaaca acgtcgcggt cgcgacgaag gccgcgcaga gagccggcgt
    13201 cgagcgggtc gcgatcgtcg actgggatct gcaccacgga aacggcacgc agcacacctt
    13261 cgagaacgac ccgaacgttc tctacttctc gacgca
//
LOCUS       DASWAV010000455         2769 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U2665
            k141_626734, whole genome shotgun sequence.
ACCESSION   DASWAV010000455 DASWAV010000000
VERSION     DASWAV010000455.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960316
            Sequence Read Archive: SRR10260007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2769)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2769)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:47:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,929
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2769
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_626734"
                     /isolate="SMAG_U2665"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.36444 N 132.31889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1132)
                     /gene="eno"
                     /locus_tag="VF958_09470"
     CDS             complement(<1..1132)
                     /gene="eno"
                     /locus_tag="VF958_09470"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012565357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004634 - phosphopyruvate hydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HEX9149376.1"
                     /translation="MSFAIEDIYAREILDSRGNPTVEVEVLLSGGATGRAGVPSGAST
                     GTREALELRDGDKKRYGGKGVTKAVANVNGEISDELRGADGRDQALIDRILIDLDGTP
                     NKGRLGANAILGVSLAVARAAAEASGLALYRYLGGAGGRTLPVPMMNLVNGGMHADNR
                     LDPQEFMVCPLGFNSFAEALRAGVETFHALKALLKKRGLSTAVGDEGGFAPDIGSAKE
                     ALDLLVAGIEAAGYRPGKDIAIALDPAASEFYRDGRYVLEGEGKTLDSTAMIAFWEAL
                     VRDYPIVSIEDGLAEGDREGWIELTRQLGGKILLVGDDNFVTNSEILARGIADGIANA
                     MLVKLNQVGTVTETLDAVKLARSSGYRTVVSHRSGETTDDSIA"
     gene            complement(1129..2526)
                     /locus_tag="VF958_09475"
     CDS             complement(1129..2526)
                     /locus_tag="VF958_09475"
                     /inference="COORDINATES: protein motif:HMM:NF014877.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucomutase/phosphomannomutase family
                     protein"
                     /protein_id="HEX9149377.1"
                     /translation="MTAIRFGTSGWRAVLAEEFTFANARRVVTAIARVLSEDGRAGEL
                     VLVGYDTRFLADRFAAEAARILAREGLGAEVASRPVPTPALAFEIRRRSAAGAVNFTA
                     SHNPPRYLGIKFSTSDGAPALPELTQRVEAAISGLPAVDPPAGTSAPPAFDPVPEYLA
                     DLGHKVDASAVAARDPHFSLDFRFGTSAGFLDAFLERAGARITRLNAKRDPLFGGESP
                     QCGEKELVHLAEEVRRSGSRLGLACDGDADRFGVCDEQGSYVPPNLVLALLLRDLLGR
                     KGRRGGVARSVATTHALDAVAAKFGVPCYETPVGFKYIGEKLIAGEIIFGGEESAGLT
                     IEGHVPEKDGILADLLAAEMCGATGRTIRELSAELDREIGPYRSTRVDLPLSPEERAR
                     LAKVKESPPDRVGSHRVASVNRIDGIKLLLDEGSWILVRESGTEPLARLYVESKSDEE
                     VAALTRAAKDLVVVA"
BASE COUNT          408 a         1002 c          931 g          428 t
ORIGIN      
        1 cggcgatcga gtcgtcggtg gtctcgccgg agcggtgcga gacaacggtt cgataccccg
       61 aggaccgggc gagcttcacc gcgtcgagtg tctccgtgac cgtcccgacc tgattgagct
      121 tgaccagcat cgcgttcgcg atcccgtcgg cgatcccgcg cgccaggatc tcggagttcg
      181 tgacgaaatt gtcgtcgccg acgaggagga tcttcccgcc gagctgccgc gtcaactcga
      241 tccatccctc gcggtccccc tccgcgagac cgtcctcgat cgagacgatc gggtaatcgc
      301 gcacgagcgc ctcccagaag gcgatcatgg cggtggagtc gagagttttc ccctctccct
      361 cgaggacgta gcggccgtcg cggtagaact cggacgccgc cgggtcgagc gcgatcgcga
      421 tgtccttgcc cggccggtag ccggcggcct cgatgcccgc gacgagcagg tcgagagcct
      481 ccttcgccga cccgatgtcg ggcgcgaatc ccccttcgtc gcccacggcg gtcgagagac
      541 cccgcttctt cagcagcgcc ttcagcgcat gaaacgtctc gacccccgcg cgcagcgcct
      601 ccgcgaaaga gttgaagccg agcggacaga ccatgaactc ctgcgggtcg agccggttgt
      661 ccgcgtgcat cccgccgttg accaggttca tcatcgggac cggcagcgtc cggccgccgg
      721 cgccgcccaa gtatcggtac agcgccagcc ccgatgcctc cgccgccgcg cgcgcgaccg
      781 cgagcgagac gccgaggatt gcgttcgcgc cgaggcgccc cttgttgggc gtgccgtcga
      841 ggtcgatcag gatgcggtcg atcagcgcct gatcgcgccc gtccgctccg cggagctcgt
      901 cggagatctc gccgttgacg ttcgcgaccg ccttcgtgac gcccttgccg ccgtagcgct
      961 tcttgtcccc gtcgcgcagc tcgagcgcct cgcgcgtgcc ggtcgaagcg cccgacggca
     1021 cgccggccct gcccgttgct ccaccggaga gcaggacctc gacctcgacc gtcgggtttc
     1081 cgcgcgagtc caggatctcg cgggcgtaga tgtcctcgat tgcgaagctc atgcgaccac
     1141 cacgaggtcc ttcgccgcgc gcgtcagcgc ggcgacctct tcatcggatt tcgattccac
     1201 gtaaaggcgc gcgagaggtt ccgtacccga ctctcgaacc agaatccacg aaccttcgtc
     1261 caaaagcaac ttgattccgt cgatgcggtt gaccgacgcg acccgatgac ttccgacccg
     1321 gtcgggcggc gattctttga ctttcgcgag gcgggcgcgc tcctccggcg agagcggcag
     1381 gtcgacgcgg gtcgagcggt agggtccgat ctcccggtcg agctccgccg acagctcgcg
     1441 gatcgtccga ccggtcgcgc cgcacatctc ggcggcgagg aggtccgcga gaatgccgtc
     1501 cttctccgga acgtgtccct cgatcgtcag gccagcgctc tcctctccgc cgaagatgat
     1561 ctcgcccgcg atcagcttct ctccgatgta tttgaatccc accggcgtct cgtagcacgg
     1621 caccccgaac tttgccgcaa ccgcatcgag cgcgtgagtc gtggcgacgc tgcgggcgac
     1681 gccgccacgg cgacccttgc ggccgagcag gtcccggagg aggagcgcga ggacgaggtt
     1741 cggcgggacg tagctcccct gttcgtcgca gacgccgaac cggtccgcgt cgccgtcgca
     1801 cgcgaggccg aggcgcgagc ccgaccgacg aacctcctcc gccaggtgga cgagctcctt
     1861 ctcgccgcac tgcggcgatt cgccgccgaa gagcggatcc cgcttcgcgt tcaggcgggt
     1921 gatccgtgcc cccgcgcgtt cgaggaaggc gtcgagaaag ccggccgacg ttccgaaacg
     1981 gaagtcgagc gagaagtggg gatctctcgc cgcgacggcg gaggcgtcca ccttgtgccc
     2041 gaggtcggcg aggtactccg ggaccgggtc gaacgccggc ggcgcagacg tgcccgctgg
     2101 cgggtcgacg gcaggcaggc ccgagatcgc cgcttccacc ctctgcgtca actccggcag
     2161 cgcgggcgcg ccgtcgctcg tggagaattt gatcccgaga tagcgtggag ggttgtggct
     2221 ggcggtgaag ttcacggcgc ccgcggcgga acggcggcgg atctcgaagg cgagcgcggg
     2281 ggtgggaacc gggcgcgagg cgacttccgc gcccagtccc tccctcgcga ggatccgcgc
     2341 cgcctcggcc gcgaaccggt ccgcgagaaa tcgcgtgtcg tagccgacga gcacgagctc
     2401 gccggcgcgc ccgtcctccg acaggacccg cgcgatggcg gtgacgacgc ggcgcgcgtt
     2461 ggcgaacgtg aactcctccg cgagcacggc tcgccagccg gaggtgccga agcgaatggc
     2521 cgtcattcgg cggtcaagct aacacgcgtc gcgggagtcg cggcggccgt cctcgcggcc
     2581 gcatgctcgg gcggccgcgc gcccgaccct cccgcgcctt cgctcacgat cgtcgcgccg
     2641 tttctcgatt cgccgatcga ggaccgtatg ccccccgcgg acgatcgccc gctcgacaag
     2701 gtcaagctcg ccgtgctcga ccggatcaac cgcgaccgcg ccgccgccgg actcccggaa
     2761 gtcctctgg
//
LOCUS       DASWAV010000456         4294 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U2665
            k141_265466, whole genome shotgun sequence.
ACCESSION   DASWAV010000456 DASWAV010000000
VERSION     DASWAV010000456.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960316
            Sequence Read Archive: SRR10260007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 4294)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4294)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:47:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,929
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4294
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_265466"
                     /isolate="SMAG_U2665"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.36444 N 132.31889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(122..505)
                     /locus_tag="VF958_09480"
     CDS             complement(122..505)
                     /locus_tag="VF958_09480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018280786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPF/RaiA family ribosome-associated protein"
                     /protein_id="HEX9149378.1"
                     /translation="MIPARIHFLDSPRSEAVEAKIQARADRLSHFSDEIQKCEVWLAS
                     PHGHHRKGDLYEVRIRMTVPNEEIEVRLQPETDDVYVAIRGAFDAARRKLEDHERRRR
                     AEVKAHPRASGDASRRRQVLSDQER"
     gene            complement(566..769)
                     /locus_tag="VF958_09485"
     CDS             complement(566..769)
                     /locus_tag="VF958_09485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013554412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dodecin family protein"
                     /protein_id="HEX9149379.1"
                     /translation="MTNTLKVIEVLAESEESWEDAAAQAVEKAAKTLNNIRSIFIKNF
                     EAKVENNKIVRYRINAKISFVLE"
     gene            complement(896..1567)
                     /locus_tag="VF958_09490"
     CDS             complement(896..1567)
                     /locus_tag="VF958_09490"
                     /inference="COORDINATES: protein motif:HMM:NF014762.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046914 - transition metal ion binding
                     [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron dependent repressor, metal binding and
                     dimerization domain protein"
                     /protein_id="HEX9149380.1"
                     /translation="MSHELDLADQDVEEVAEELWTLAEQGSDRLSDLRDTTRVEALYP
                     ALEELARRGLVRLEENHVSLTPQGRELAARQVRRHRLAEALFTTVLEVHDDEAVNQTA
                     CVMEHVLSGGIADSICAFLGHPVICPHGKPIPPGACCGSFTRTITPLVQPLDQLAVGE
                     EGRVVFISPKVPERMTRLSNLGVVPGAIVRLQQRRPAAVLRVGETTLALDSEAAGEIY
                     VKKVS"
     gene            complement(1564..1821)
                     /locus_tag="VF958_09495"
     CDS             complement(1564..1821)
                     /locus_tag="VF958_09495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9149381.1"
                     /translation="MAMNPEEITQVPFVATTSESAVGTLFSCPLCGGRFSHGQEVCSA
                     CPLRPRCNVVGCPHCGYQFPRTSATVEMLGRLVRWLSRRRT"
     gene            complement(1812..3830)
                     /gene="feoB"
                     /locus_tag="VF958_09500"
     CDS             complement(1812..3830)
                     /gene="feoB"
                     /locus_tag="VF958_09500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012097036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015093 - ferrous iron transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein B"
                     /protein_id="HEX9149382.1"
                     /translation="MKGRQATSAAREPAELAVAPPPPEPGNDVVLVGNPNVGKSALFN
                     ALTGAYVTVSNYPGTTVEFTSGSAVLDGRRTGITDTPGIASFLPSSEDERVTRDILLL
                     ERHRAVVLVGDAKNLERTLLLGVQLAEMELPFVLCLNMMDEAAGRGITIQAEELARHL
                     GVDVVPTVAVRREGITRLVRALAAPRRGSVLVEYSDTIEQAIGAIAPLLPQAPISPRS
                     LALLVACGDETLHAWLKERMPGADLRRLDEIRQKLRHAIGGDVAHAVNRARSRAARRL
                     VNGAASRLGRERARNRWAVRLEELTTHPVWGVPILGAALYLAYLFVGKLGAGTLVDLL
                     ENRLFGGVISPAAVAAANRWIPWAFVRDILVGPYGVITMALAYSLALLLPIVTTFFIA
                     FGVLEDSGYLPRLAVMVNRLFKKMGLNGKAVLPMVLGLGCDTMATMTTRILETPKERL
                     IVIMLLALGVPCSAQLTVVLAMLGGVALWATLVWAVVIGGVLVLVGRLAARVLPGRGS
                     DFVLELPPLRLPRPGNILVKTLARVEWYLKEAVPLFVLGTLLLFFADRLHLLGFVERA
                     ARPVISGWLGLPAETAEAFVVGFLRRDFGAAGLFRLARSGALDPIQIVVAMVTITLFI
                     PCIANFFMIVKERGWKTACAIAAFILPFTLLVGGLLNAFLRAVPGPWR"
     gene            3972..>4294
                     /locus_tag="VF958_09505"
     CDS             3972..>4294
                     /locus_tag="VF958_09505"
                     /inference="COORDINATES: protein motif:HMM:NF026066.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HEX9149383.1"
                     /translation="MSKNGAADLVDPRDTPLVNDVVARRLEEVARLLEAQGADPFRVR
                     AWRSGASRVRRLDRPAEVILREAGIAGLERLPDIGPVISRAIKSLVITGRLPMLERLR
                     GEMDP"
BASE COUNT          725 a         1415 c         1467 g          687 t
ORIGIN      
        1 ccgactgaca ctccgtgact cgaggcgcag gtccgggagc agtagagggc tccatcggtt
       61 cccaggaagc cgatcacgtc ctgttcgtcg tccacgttat tccggcgtcc taacgcacgc
      121 tctatcgttc ctgatcgctg aggacctgcc ggcggcgcga ggcgtcgcca gaggcccgcg
      181 gatgcgcttt cacctctgcg cggcggcgcc gctcgtggtc ttcgagcttg cgccttgcgg
      241 cgtcgaatgc tccgcgaatc gcgacgtaga cgtcatcagt ctcgggctgt agacgaactt
      301 caatctcctc gttcggcacg gtcatccgga ttcggacctc gtagagatcg ccttttcggt
      361 ggtgaccgtg cggtgacgcg agccagacct cgcacttctg gatctcgtcg gaaaaatgcg
      421 agagacggtc ggcccgcgcc tggatcttcg cctcgacggc ttccgaacgg ggactgtcga
      481 ggaagtgaat gcgggcaggg atcatggttt tctccttccg atggtgtcgg cgcccgtcgg
      541 ccacctgtct cgcaggaatg ccgagctact cgagaacgaa ggagatcttg gcgttgatcc
      601 ggtagcggac gatcttgttg ttctccacct tcgcctcgaa gttcttgatg aagatcgagc
      661 ggatgttgtt caacgtcttg gcggcctttt ccaccgcctg cgcggccgcg tcctcccagc
      721 tttcttccga ttcggcgagc acctcgatga ctttgagagt gttggtcata cagcctcctc
      781 cgcatcggtc ggcaggatcc cgaccgatga cgcgtgaaga ggattcccca gacttcggcc
      841 ccgcggcaga tccgttcgca tcaggccacg cccggcaacg gtcctccccg ggctttcagc
      901 tcactttctt gacgtagatc tctccggcgg cctcggaatc gagggcgagc gtggtctcac
      961 cgacccgaag gacggcggcg ggtcgccgct gctggagacg cacgatcgcc cccggaacga
     1021 ctccgagatt cgacagccgt gtcatccgtt cgggcacctt tggcgagatg aagaccaccc
     1081 gtccctcctc gccgacggcc agctggtcga gcggctggac gaggggcgtg atcgtgcgcg
     1141 tgaacgaccc gcagcaggct cccggcggta tcggcttgcc gtgagggcag atgacggggt
     1201 gcccgagaaa cgcgcagatc gaatcggcga tcccgcccga gaggacgtgt tccatcacgc
     1261 acgcggtctg gttcaccgcc tcgtcgtcgt ggacttcgag aacggtcgtg aagagcgcct
     1321 cggcgagccg gtggcggcgg acctggcggg cagccagctc gcgcccctgc ggagtgagcg
     1381 agacgtggtt ctcttcgagc cgcacgagtc cgcgccgcgc gagctcctcc agcgcggggt
     1441 acagcgcctc gacccgcgtc gtgtcccgca ggtccgagag ccgatcggat ccctgctccg
     1501 cgagcgtcca gagttcctcg gcgacttcct cgacgtcctg atccgcgaga tccagctcgt
     1561 gactcatgtt cttctcctgg aaagccaccg gacgagacgg cccagcattt cgacggtggc
     1621 cgaggtgcgg gggaactggt aaccgcagtg cgggcagccg acgacgttgc atcgcggcct
     1681 gagcgggcag gcgctgcaca cttcctgtcc gtgcgagaag cgtcccccgc agagcgggca
     1741 cgagaagagg gttcccaccg ccgactccga ggtcgtcgcg acgaacggga cctgcgtgat
     1801 ctcttccgga ttcatcgcca cggccccgga accgctcgca ggaatgcatt gaggagaccg
     1861 ccgacgagga gcgtgaacgg caggatgaag gcggcgatcg cgcacgccgt tttccatccg
     1921 cgctccttga cgatcatgaa gaagttcgcg atgcacggta tgaagagcgt gatcgtcacc
     1981 atcgcgacga cgatctggat cgggtcgaga gcgccggagc gcgcgagccg gaagagaccg
     2041 gcggcgccga aatcgcggcg caggaatccg acgacgaacg cttccgcggt ctcggcgggc
     2101 aggccgagcc agccggagat gacgggccgt gccgcgcgct cgacgaaacc gagcaggtga
     2161 aggcggtcgg cgaagaagag gaggagcgtc ccgagcacga agagagggac cgcttccttc
     2221 aggtaccact cgacccgcgc cagggtcttg acgaggatgt tcccgggccg aggcagccgc
     2281 agcggcggga gctccaggac gaaatcgctc ccgcgcccgg ggagcacgcg cgccgccagc
     2341 cggccgacga ggacgagcac ccctccgatg acgacggccc agacgagtgt cgcccacagc
     2401 gccaccccac ccagcatcgc gagaacgacg gtgagctgcg ccgagcaggg cacaccgagc
     2461 gccaggagca tgatcacgat caggcgctcc ttgggcgtct ccaggatccg cgtcgtcatc
     2521 gtcgccatcg tgtcgcatcc gaggccgagc accatcggca atacggcttt tccattcaag
     2581 cccatcttct tgaagagccg gttcaccatc accgccaggc gcggcaggta tcccgagtcc
     2641 tcgagaacgc cgaacgcgat gaagaacgtc gtcacgatcg ggaggaggag cgcgagcgag
     2701 taggcgaggg ccatcgtgat gacgccgtag gggccgacga ggatgtcgcg cacgaacgcc
     2761 cacggaatcc accggttcgc ggccgcgacc gcggcggggc tgatgacgcc gccgaagaga
     2821 cgattctcga ggagatccac gagagttccc gcgcccagct ttccgacgaa gagataggcg
     2881 agatacagcg cggcgccgag gatcggaaca ccccacaccg gatgcgtcgt cagctcctcc
     2941 agccggacgg cccaccggtt ccgcgcgcgc tctctcccga gccggctcgc cgcgccgttg
     3001 acgagccgcc gcgcggcgcg cgaccgggcg cggttgacgg catgggccac gtcaccgccg
     3061 atcgcgtggc ggagcttctg gcggatttcg tccagccgtc ggagatcggc tccgggcatc
     3121 cgctctttga gccaggcgtg cagagtctcg tctccgcagg cgacgaggag cgccagactg
     3181 cgcggcgaga tcggggcctg cgggagaagc ggggcgatcg cgccgatcgc ctgctcgatc
     3241 gtgtcggagt attcgacgag gacgctgccc cgccgggggg ccgcgagggc ccgaacgagg
     3301 cgagtgattc cctcgcgacg cacggcgacg gtcggaacga cgtcgacccc gaggtgccgg
     3361 gcgagctcct ccgcctggat ggtgattccg cgccccgcgg cttcgtccat catgttgagg
     3421 cagagcacga acgggagctc catctccgcg agctgcacgc cgagaagaag cgtcctctcg
     3481 aggttcttcg cgtccccgac gagcacgacc gcgcggtgtc gctcgagcag caggatgtcc
     3541 cgggtcaccc gctcgtcctc cgacgagggg aggaaagatg cgatgcccgg cgtgtcggtg
     3601 atccccgtgc gccggccgtc gagaaccgcg gagccggacg tgaattcgac cgtcgtgccg
     3661 ggatagttcg agacggtgac gtaggcgccg gtcagcgcgt tgaaaagagc gctctttccg
     3721 acgttcggat tgccgacgag gacgacgtca ttgccgggtt cgggcggcgg gggagccacc
     3781 gcgagctccg ccggctcgcg cgcggccgaa gtcgcttgtc ggcctttcac gtcgtgagcg
     3841 tagggggcgg gagaccgact ccactgatgc aaatgaatca gccctcctcc ggcccatcga
     3901 cgccctcaag ggtcttttga ttcaaccgga tcaggcgtaa tcgcgcgaag tggagcagag
     3961 tcacggtgac gatgtcgaag aatggcgccg ccgacctggt ggatcctcgg gataccccgc
     4021 tcgtcaacga cgtggtggcc cggcggctcg aggaggtcgc ccgcctcctc gaggcccagg
     4081 gcgccgatcc gttccgcgtc cgggcctggc gttcgggcgc ctcgagggtt cgacggctcg
     4141 accggcctgc agaggtgatc cttcgcgagg cggggatcgc cggcctggaa cgtcttccgg
     4201 acatcggccc ggtgatctcg cgcgcgatca agagcctcgt gatcacgggg cgccttccga
     4261 tgctcgagcg acttcgcggg gagatggacc cgga
//
LOCUS       DASWAV010000457        13436 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U2665
            k141_465557, whole genome shotgun sequence.
ACCESSION   DASWAV010000457 DASWAV010000000
VERSION     DASWAV010000457.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960316
            Sequence Read Archive: SRR10260007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 13436)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13436)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:47:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,929
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13436
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_465557"
                     /isolate="SMAG_U2665"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.36444 N 132.31889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..491)
                     /locus_tag="VF958_09510"
     CDS             complement(<1..491)
                     /locus_tag="VF958_09510"
                     /inference="COORDINATES: protein motif:HMM:NF012579.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HEX9149384.1"
                     /translation="MAGAEAGFFSRLVDERLVFPDLAGATLEEALREMSSGLARAGVV
                     RDPEDLERHLIERESMGSTGLGGGLAIPHCKVRDLADVVLSVGVSRSGIDFRAADGIP
                     VTLIFLVVSPADAPALHLQALARLSRVIRIPGVAESLRSASTTREIAEALRAAERQLA
                     VPA"
     gene            complement(508..1242)
                     /locus_tag="VF958_09515"
     CDS             complement(508..1242)
                     /locus_tag="VF958_09515"
                     /inference="COORDINATES: protein motif:HMM:NF014534.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPF/RaiA family ribosome-associated protein"
                     /protein_id="HEX9149385.1"
                     /translation="MRASCRSCARRGSRSRAAPSRSTVRSCGFPRRRSESRVFEPGGL
                     PRTPHYQRHGGMMAVECTGRNVEVTPSLISLARERTQRLERHLGGPAKVRVVLSQEKH
                     RFGAEIIATHRRRRWNAHEYTVDPRAAVALAFEKIDAQAKKDSEKRRKRKDRSSPRLL
                     PATPLPAPEAAARRSRTVPSATSSEPQRRIVRANRLSLKPMSTEEAVLRMEDSRREFL
                     VFRDAGTEKISVLYKRRDGNLGLIEC"
     gene            complement(1124..2584)
                     /gene="rpoN"
                     /locus_tag="VF958_09520"
     CDS             complement(1124..2584)
                     /gene="rpoN"
                     /locus_tag="VF958_09520"
                     /inference="COORDINATES: protein motif:HMM:NF009118.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HEX9149386.1"
                     /translation="MALEQKLNLRLSQRLVMTPSLQQAIKLLQMSKLELQDVLTQEIV
                     ENPLLEEEEESAAEPEPAVEAESAKEAEAPSAAADAGETPPEKERDSFDEIDFDSYFE
                     DYLDSAYNPRQYEDSEQVPLENTLSRPQGLHEYLNWQLALADDAPGVREIAQYLIGNI
                     DEDGYLRVSREEIRAAGYENDEDVESALALVRSFDPPGVGAFDLPDCLMMQIRTLGIE
                     NALIETIITEHWGEFLNRQFVQLSRTLNVGLSQLQAVVEIIKNLEPKPGRKYSDERTI
                     YVEPDVAVRKIGDEYVIQLNEDGLPKLRISAAYRRMLRGGNGAIGEEAATYLRDKMRS
                     AVWLIKSLDQRQRTIYKVADSIVRHQKGFLENGIEHLRPLVLRDVANDIGMHESTVSR
                     VVSNKYIHTPRGLFPMKYFFHSGIDSTQGAEVSSLSIKSKIAKIVSEEDAARPHSDAR
                     IMQKLRAEGIQIARRTVAKYREELRIPSSSQRKQSF"
     gene            complement(2600..3340)
                     /gene="lptB"
                     /locus_tag="VF958_09525"
     CDS             complement(2600..3340)
                     /gene="lptB"
                     /locus_tag="VF958_09525"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter ATP-binding protein"
                     /protein_id="HEX9149387.1"
                     /translation="MDASSPPRLVAEGLVKSYGGRTVVRGVSIDIAPGEIVGLLGPNG
                     AGKTTTFSIVVGLVEPDEGTVRLGDRDLTGLPMYRRARAGISYLPQEPSVFRKMTVEQ
                     NLLAILETLDLPHSAQVERAGALLSEFGLEAVRDHRAFTLSGGERRRVEIARSLVVSP
                     YFLLLDEPFAGIDPIAVLDIQSIVRRLASSGIGVLVTDHNVRETLSIVDRAYIINAGE
                     ILRAGTPLALSNDAVVRKIYLGEAFQLR"
     gene            complement(3453..5429)
                     /gene="lptC"
                     /locus_tag="VF958_09530"
     CDS             complement(3453..5429)
                     /gene="lptC"
                     /locus_tag="VF958_09530"
                     /inference="COORDINATES: protein motif:HMM:NF018537.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter periplasmic protein
                     LptC"
                     /protein_id="HEX9149388.1"
                     /translation="MKLSRLEIAILFFGIAFLALVAAFFRPGHRVRNERPSAVENVPA
                     GRDAGEAATTLLSGFDYTESVGEKPLFRIRSDRTVGYGTGAGLPPNRYALEKVSLTVY
                     PEDGAPVTVNAERAQYDDRTKAAVLSGNVHWVDAEQGGLGETDRIEFEPSKRVMTAPA
                     PLHFTRGTFDVHASSGAYDVRRRVLTLAGPIQGSGTGQGSGGLSSLAGDSGEYRRDEG
                     VVELRGNVSASSTKGDRIACDRLLLKFSPEGNRSEWARALGAVHGSVAPSASGGGERS
                     YSADEGILHFDDAGDVREISLTGSPASVSERERDLSARSVDIEFTAGRPASARASGEV
                     QVRSDRGNANSQRARAVFGPSGGFQSLELEGEVRIEGDGRTGLADRVVDVADRDVWLL
                     TGSPGKAATVEEKGSKVSGDRIEIDRGRKALRAEGRARAVFVPEKDRPADASSLLGDP
                     TRPTFGKADRIVLDRETHLASLSGGASLWQESSSVFGDDITLNDVEKSAVAVGKVRAV
                     LSRAAVSGPATPPKEKEEPAIITARRLIYRETESTAVFEDSVTATRGPWRATAARGVA
                     FFGTDKKLDHVELTGTVTLDDRSAGRHATADRAEDFPKEGRTVLHGNPARVTDAEGNR
                     VAGSILTITGRGKNVEVTASEGGRTETVHKTKAD"
     gene            complement(5426..7219)
                     /gene="recJ"
                     /locus_tag="VF958_09535"
     CDS             complement(5426..7219)
                     /gene="recJ"
                     /locus_tag="VF958_09535"
                     /inference="COORDINATES: protein motif:HMM:TIGR00644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008297 - single-stranded DNA
                     exodeoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HEX9149389.1"
                     /translation="MEPIRAVISDLVARLRSTSPRDPADALARQAKLPRVVAELLLAR
                     GIGTAEDAALFFRPDESQLHDPFRMRGVEEAAAILLSAARAGRRIVVFGDYDVDGVTA
                     VAQLKAALARAGATALSFIPHRLKDGYGLRPDTVRRVLSELAPSVIVTVDCGIGAIEG
                     VACAREAGVEVVVTDHHLVPDRLPAGAVVINPKQPGCEYPYKELSGSGIAFKLSEAIA
                     LRAGVPLSRDALLRAACLGTIADIVPLTGENRAIAALGLQALARPKAPGLAALLEACG
                     IEAGSAPTSEEVAFRIAPRLNAAGRLDTAELALAVFEERDPAKGAAIARELCARNAER
                     QSVERRVFAEARERVAKTFDPERDFLIVEGDPSWHRGVLGIAASRLAREYNRPVLLFA
                     LEGERASGSGRSIPGVPLHDTLNEMRYRFLDFGGHDQAVGGSLAAAGFESFREDARAL
                     FARRIAPERLERREASDAELPLDEIRPDLTGELARFEPHGAGNPRPVFSCAGVRAALP
                     FRPLGESGWRGRLETAHGSIDAICWNERAGIGRLSASGEFLSLHYRLSESRWTGRPEV
                     EIVAARADGIPEENEDRLPSPVSRLPSPAAP"
     gene            complement(7273..8235)
                     /locus_tag="VF958_09540"
     CDS             complement(7273..8235)
                     /locus_tag="VF958_09540"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014238178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit alpha"
                     /protein_id="HEX9149390.1"
                     /translation="MNGEDGQFEEPILRLRQRIEELSALPDDAAHRKEIERLREKLDR
                     TSREIYAGLTPWQKTLVARHPTRPYTLDYIGALMTDFVELHGDRKFAEDAAVVAGLAR
                     FRGRPVAIVGHQKGRDTKEKIRRNFGMPRPEGFRKALRVMQLAEKFERPIISFVDTAG
                     AFPGIDAEERGQAEAIAVNLRSMAGFRVPIIVAVTGEGGSGGALALSVGDRILMLEHA
                     IYSVISPEGCAAILWKDASRKKEAAAALRVTAGDLAEFGIIDEIVPEPPGGAHADPTS
                     AAAALGEAIERHLREVSDLTTEERRERRYRKFRAMGRFESQPVG"
     gene            complement(8433..10868)
                     /gene="priA"
                     /locus_tag="VF958_09545"
     CDS             complement(8433..10868)
                     /gene="priA"
                     /locus_tag="VF958_09545"
                     /inference="COORDINATES: protein motif:HMM:TIGR00595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005658 - alpha DNA polymerase:primase
                     complex [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HEX9149391.1"
                     /translation="MRTVSVALPVPIRRNFTYRVPEHLDVPAPGMRVRVPFGERVLTG
                     VVVPDREDAGGGAHDASLRDILDVLDEEPVCPPELLETAARVAQRFFAAEGEVLKSAL
                     PARLPAAGAVRYRITEKGALGAGRSQGEERAFLDAIADGRHVPLSELPRPARPRREVL
                     RLLEERGWVRAASQDRRAARRPELAYLPGRLDVAEREERLARSRRGREVFEYLESLGR
                     PATGREIRVATGAGPGVLRSLASKGVLATFEQLEREEPVLSPVAPPGRPDVVLTEEQA
                     RALETVRESIRSRHGRAALLQGVTGSGKTEVYLRAIASALELGRGAVWLVPEIALTPV
                     FARQLKRQFGDRAAVLHSALSEKERARAWDRVRSGEAPVVIGPRSAAFAPVADPGIFV
                     VDEEHDASYKQRENPRYDARDVAAIRAKANGAALLMGSATPSMEAYYSASVGRMTSLR
                     LASRVESRPLPQVAIVDLRRERATPEEKGVALFSVALVNRLTEVFSRGEQAILLQPRR
                     GFAPFLLCRDCGHDFRCSRCSVSRTVHDRGRRLVCHYCGERVPRPLRCPQCGGALLEA
                     IGAGTERVAERFAVLFPEVPWTILDRDAARRRGVEAVVDDVLSGRARCLIGTQMVAKG
                     HDFPNVTAVGVLSADSILNFPDFRAAEKTFQLLAQVAGRAGRGNRPGTVHVQTFHPDH
                     PAIRRAAVHDVDGFAEQELEFRRAFFYPPFAELASVLVSSADREKAEELAAEIGRALS
                     ASGSKGSGGKGVRLSGPAPAPLERLQGKWRFQILVRAAERSVVLSMLGEAIPERPPSG
                     TQIGVDVDPQDLM"
     gene            complement(10907..11503)
                     /locus_tag="VF958_09550"
     CDS             complement(10907..11503)
                     /locus_tag="VF958_09550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9149392.1"
                     /translation="MTLACGVALLAGLLGCRRPPAAPRIANSDRVARPPAPPAGSGYS
                     FRPEEKEAVEAFLRANRDLRMATDDDRRPSESDDLGKLYTVYHPYFLRGDVNDDGILD
                     FVLAFVKRDSDRDSPWFSVVAFPGKEGAGFGPGTFIERDVSLADGDLSVDRDSIVITP
                     DLDEDENTRRYRWDPLKRSYVFVRDDALGDEGVSSAQT"
     gene            11727..13109
                     /locus_tag="VF958_09555"
     CDS             11727..13109
                     /locus_tag="VF958_09555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011734494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate ammonia-lyase"
                     /protein_id="HEX9149393.1"
                     /translation="MRQEKDSLGVREIPDEAYWGAQTSRAIENYPISGERAHPEMIRA
                     YARIKKACAETNLEMGGLPAEKVRAIVQACDEVLAGRHFDQFVVDVYQAGAGTSFHMN
                     VNEVIAGRAAELAGASRGDRKAINPNDDVNLGQSTNDTFPTALHMAAIAVGRVLVAEV
                     EKLAASFERKGKEFDGIVKSGRTHLMDAVPVTLGQEFRAFASAISHRREGLTRALDRL
                     RELPLGGTATGTGINTAAGFREKAIEKLARLAGEAFSPATDAREGLQSRAAAGDYSAA
                     LRSSAVELGRIANDLRLLASGPATGLDEVRLPAVQPGSSIMPGKVNPSLLECLNMLCY
                     QIVGFDTANLLAVGGGQLDLNVMMPLLAYNLTRGPQLLINFLPVVRQRCIDGITANVE
                     KCRLYLEESPSVVTALTPKIGYATAAEVFKEAVARGVLVKQVLLEKKILTEDELAAAM
                     TKEALLGPLE"
     gene            13168..>13436
                     /locus_tag="VF958_09560"
     CDS             13168..>13436
                     /locus_tag="VF958_09560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010510328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9149394.1"
                     /translation="MTWPQDWQTNATFLLRWVHLLAGITWIGLLYWFNLVNVNFQKEI
                     DAAVKGKVNPPLLSRSLWWFRWGAIVTWLTGAAYYGILLGTENIGA"
BASE COUNT         2136 a         4609 c         4591 g         2100 t
ORIGIN      
        1 caagcgggca cggcgagttg cctctcggcg gcccgcaggg cttcggcaat ctcgcgggtg
       61 gtggacgcgg aacgaaggct ctcggcgact cccggaatcc ggatcacccg cgagagccgg
      121 gcgagcgcct gcaggtgaag ggccggagcg tccgcgggag agacgacgag gaagatcaac
      181 gtcacgggga tgccgtcggc ggcccggaaa tcgatccccg atcgcgatac cccgaccgag
      241 aggacgacgt cggcgaggtc gcgcaccttg cagtgcggga tcgccagccc gccaccgagt
      301 cctgtggaac ccatggactc gcgctcgatc aggtgccgct cgaggtcttc ggggtctcgc
      361 acgaccccgg cgcgcgcgag gccggacgac atctcccgga gcgcttcttc cagggtcgcc
      421 cccgcaagat cagggaaaac cagccgctcg tcgacgagcc gggaaaagaa tcccgcctcg
      481 gctccggcca tcagcgccgg gtcccgttca gcattcgatg aggccgaggt tcccgtcgcg
      541 gcgcttgtag agcaccgaga tcttctccgt gcccgcatcc cggaagacga gaaactcccg
      601 tcgcgagtct tccattctca acacggcttc ttcggtgctc atcggtttca gggagagcct
      661 gttcgcgcga acgattcgcc gctgcggctc ggaagacgtg gcggagggaa ccgttcggga
      721 gcggcgcgca gcggcctccg gtgcgggtag cggcgtcgcg ggcagcaacc gcggcgacga
      781 ccggtcctta cgcttgcggc gcttttcgga atccttcttc gcctgcgcgt cgatcttctc
      841 gaaagcgagc gcgaccgctg cccgcgggtc gaccgtgtac tcgtgagcat tccagcgccg
      901 cctccggtgc gtcgcgatga tctccgcgcc gaaacggtgc ttctcctgcg agagaacgac
      961 gcgtactttg gccggaccac cgaggtgacg ctccagccgc tgcgtccgtt ccctcgcgag
     1021 gctgatcaaa gatggagtga cttcgacgtt ccgaccggta cattcgaccg ccatcatccc
     1081 tccgtgacgc tggtagtgcg gggttcgggg caaacccccc ggctcaaaaa ctctgctttc
     1141 gctgcgacga cgagggaatc cgcagctcct cacggtactt cgcgacggtg cggcgcgcga
     1201 tctggatccc ctccgcgcgc aacttctgca tgatgcgcgc atcggaatgg ggccgtgccg
     1261 cgtcctcctc cgagacgatt ttcgcgatct tgctcttgat cgagagcgac gagacctccg
     1321 ctccctgcgt cgagtcgatt ccgctgtgga agaagtactt catcgggaac aggccgcgag
     1381 gcgtgtggat gtacttgttc gacacgacgc gggagacggt ggattcgtgc attccgatgt
     1441 cgttcgccac gtcgcgaagc accagcggcc gcaggtgttc gatgccgttc tccaggaagc
     1501 ccttctggtg ccggacgatc gagtccgcga ccttgtagat cgtccgctgg cgctggtcga
     1561 gactcttgat cagccagacg gcggatcgca tcttgtcgcg caggtacgtt gccgcttcct
     1621 cgccgatcgc gccattgcct ccccggagca tccgccggta cgccgcggag atgcgcagct
     1681 tcggcagacc gtcttcgttc agctggatca cgtactcgtc gccgatcttc cggaccgcga
     1741 cgtccggctc gacgtaaatg gtccgttcgt ccgaatactt tcggccaggc ttgggctcga
     1801 ggttcttgat gatctcgacg accgcctgga gctgcgacag cccgacgttc aacgtccgag
     1861 agagctgcac gaactgccgg ttcaggaact ccccccagtg ctcggtgatg atcgtctcga
     1921 tgagtgcgtt ctcgatgccg agcgtccgta tctgcatcat gagacagtcg ggaagatcga
     1981 aagcgccaac gcccggagga tcgaaggacc ggacgagcgc gagcgccgat tcgacgtcct
     2041 cgtcgttctc gtacccggcc gcgcggatct cctcgcgcga cacgcgcaaa tagccgtcct
     2101 cgtcgatgtt gccgatcagg tactgagcga tctcgcggac ccccggggcg tcgtcggcga
     2161 gcgccagctg ccagttcagg tactcgtgca gcccctgcgg gcgcgacagc gtgttctcga
     2221 gaggcacctg ttcgctgtcc tcgtattgac gcgggttgta ggcgctgtcg aggtagtcct
     2281 cgaagtacga gtcgaagtcg atctcgtcga aagagtcgcg ctccttctcc ggcggagtct
     2341 cacccgcatc ggcggccgcg gagggcgcct cggcctcctt cgcggactcg gcctccacag
     2401 ccggctcggg ctccgccgcg gactcttctt cctcttcgag gagcgggttt tcgacgatct
     2461 cctgggtcag aacgtcctgg agctcgagct tggacatctg caacagcttg atcgcctgct
     2521 gcagcgacgg cgtcatgacg agccgctgag aaagtctgag gttgagcttt tgttcgagtg
     2581 ccatcagaga tgaaaagggc tagcgaagct gaaacgcctc tcccagataa atctttctta
     2641 cgacggcgtc attcgacaag gcaagcggcg tgccagcccg caaaatctcg ccggcgttga
     2701 ttatataagc gcggtcgacg atcgacaaag tctcgcggac gttgtgatcc gtgaccagaa
     2761 caccgattcc ggaggaagcc aggcggcgca cgatcgactg gatgtcgagc accgcgatcg
     2821 ggtcgatccc cgcgaacggc tcgtcgagca gcaggaagta tggagaaacg accaggctgc
     2881 gcgcgatctc gacccggcgg cgctcgccgc ccgacagcgt gaaggcacgg tggtcgcgaa
     2941 ccgcctcgag cccgaattcc gagaggagcg cccccgcccg ctcgacctgg gcggagtgag
     3001 gcaggtcgag agtctccagg atcgcgagca gattctgttc gacggtcatc ttccggaaga
     3061 ccgagggctc ctgcggcaga tacgagatgc cggctcgcgc ccgccggtac attggaagac
     3121 ctgtcagatc gcggtcgccg agacggaccg tcccttcgtc cggctcgacc aatccgacca
     3181 cgatggagaa cgtcgtggtt tttccggccc cgttgggacc gagcaggccg acgatttcgc
     3241 ccggggcgat gtcgatggat acgccgcgaa cgacggtgcg gccgccgtag gacttgacca
     3301 gaccttccgc gacgaggcga ggaggggagg acgcgtctat cgcggcggaa tccgttccct
     3361 caccccgccc ctctcccgga ggcagaggga atatgcggcc ccgattcttg tcgtccacgg
     3421 acctaggata gtcccgcgcg actcgggcga gctcagtcgg ctttggtctt gtgcaccgtt
     3481 tccgtccgtc cgccttcgga agcagtgact tcgacgttct ttccgcgtcc ggtaatcgtc
     3541 aggatcgacc cggccactcg gttgccctcc gcgtcggtga ccctcgccgg gttgccgtgg
     3601 agaaccgttc gcccctcctt tggaaagtct tcggctcgat ccgcagtcgc gtggcgaccg
     3661 gcggagcggt catcgagcgt cactgtgccc gtgagctcga cgtgatcgag cttcttgtcc
     3721 gttccgaaga acgcgactcc ccgcgcggcg gttgcccgcc aggggccgcg cgtcgcggtc
     3781 accgagtctt cgaagacggc cgtagattct gtctcacggt agatgaggcg ccgcgccgtg
     3841 atgatcgcag gctcctcctt ctccttcggc ggcgtcgcgg ggccggagac cgccgcgcgg
     3901 gagaggaccg cgcggacctt tcccaccgcg accgcgctct tctccacgtc gttcaacgtg
     3961 atgtcgtcgc cgaagaccga ggaggactcc tgccacaggc tcgctcctcc cgacagcgac
     4021 gcgaggtggg tttcccggtc gagcacgatg cgatcggctt tcccgaaagt gggccgcgtg
     4081 ggatcgccga gaagggacga ggcgtcggcg ggccggtcct tctccggaac gaagactgct
     4141 cgcgcccgcc cctcggcgcg gagcgctttg cgaccgcgat cgatctcgat tcgatctccg
     4201 gacactttcg agcctttctc ctcgaccgtc gccgccttcc ccgggcttcc ggtgagcagc
     4261 cagacgtcgc ggtccgcgac gtccacaacc cggtccgcga ggccggtcct cccgtccccc
     4321 tcgatgcgga cttcgccttc gagctcgagc gattgaaagc cgccggaagg tccgaagacg
     4381 gcgcgggcgc gctgagaatt cgcgtttccg cgatcggagc ggacttgcac ctcgccgctt
     4441 gcgcgcgccg acgcggggcg cccggccgtg aactcgatgt cgacggagcg ggccgagagg
     4501 tcgcgctcgc gctcgctcac ggaggcgggc gaccccgtca gactgatctc gcgcacatcc
     4561 ccggcgtcgt cgaagtggag gattccctca tcggccgagt aggagcgctc cccaccgccc
     4621 gatgccgacg gagcgaccga tccgtgcacg gcgcccaacg cgcgcgccca ctcggaccga
     4681 ttcccttccg gcgagaactt gaggagcagc cggtcgcagg cgatccggtc gcccttcgtg
     4741 gaggaggcgg acacgttgcc ccggagctcg acgaccccct cgtcgcggcg gtactcgccc
     4801 gagtcgccgg cgagcgagga gagaccgccg ctcccctgcc cggttccgga gccctggatc
     4861 gggccggcga gcgtcagcac gcggcgcctc acgtcgtacg ctcccgatga ggcgtgcacg
     4921 tcgaaggtgc cgcgcgtgaa gtgaagcggc gcgggagcag tcattaccct cttcgacggt
     4981 tcgaactcga tccggtccgt ctcgccgaga cccccctgct ccgcgtcgac ccagtgaacg
     5041 ttccccgaga ggaccgcggc cttcgttcgg tcgtcgtact gcgcgcgctc cgcgttcacg
     5101 gtgacgggtg cgccgtcttc cgggtagacg gtgagcgaga ccttctcgag cgcgtagcgg
     5161 ttcgggggaa ggccggcgcc cgtcccgtat ccgaccgtgc ggtccgagcg gatgcggaag
     5221 agcggtttct cgccgacaga ctcggtgtag tcgaaccccg agaggagagt cgtggcggcc
     5281 tcgcccgcgt cgcgccccgc gggaacgttc tcgaccgcgc tcgggcgctc gttgcgcacg
     5341 cggtgtccgg gtcggaagaa cgctgccacg agagcgagga aggcgatccc gaagaaaagg
     5401 atcgcgatct cgaggcgcga aagcttcacg gagccgccgg ggacgggaga cgggagacgg
     5461 gggacgggag acggtcttcg ttctcctcgg ggatgccatc ggcgcgcgcc gcgacgatct
     5521 cgacttccgg ccgcccggtc cagcggctct ccgacagccg gtagtgcaga gaaaggaact
     5581 cgccggaggc gctcaatcgg ccgatgcccg cccgctcgtt ccagcagatt gcatcgatcg
     5641 acccgtgcgc ggtctccagt cggccgcgcc atccgctctc gccgaggggg cggaagggaa
     5701 gggcggcgcg cacgccggcg caggagaaca cgggccgcgg gttcccggcg ccgtgcggtt
     5761 cgaatcgggc gagctctccg gtcaagtccg ggcggatctc atcgagcggc agctccgcgt
     5821 cggaggcttc ccgccgctcg agacgctcgg gcgcgatgcg acgggcgaag agcgcccggg
     5881 catcctcgcg gaacgactca aagccggcgg ccgcgagcga gcctcccacg gcctggtcgt
     5941 gtccaccgaa atccagaaag cgataccgca tctcgttcaa cgtgtcgtgg agcggaactc
     6001 cgggaatgct cctccccgaa ccgctcgcgc gctccccctc cagagcgaag aggagaaccg
     6061 ggcggttgta ttcccgcgcg agccgggagg ccgcgatccc gagcacgccg cggtgccacg
     6121 aaggatcccc ttccacgatc aggaaatccc gttccggatc gaaggtcttc gcgacgcgct
     6181 cgcgcgcctc cgcgaagacc cgccgttcga cggactgccg ctccgcgttg cgcgcgcaga
     6241 gctcccgcgc gatcgcggcg cccttcgcgg ggtcccgctc ctcgaacact gccagcgcga
     6301 gctccgcggt gtcgagccgg cccgccgcgt tcaagcgtgg agcgatccgg aaagcgacct
     6361 cctccgaggt gggcgccgat cccgcctcga tcccgcacgc ttccaggagc gcggcgagcc
     6421 ccggcgcttt cggtcgcgcg agcgcctgga gcccgagcgc tgcgatcgcg cggttctcgc
     6481 cggtcagcgg gacgatgtcc gcgatcgtgc cgagacaggc cgctcgcagc agcgcgtcgc
     6541 gcgagagggg aacgcccgcg cgcagcgcga tcgcctccga gagcttgaag gcgatgccgg
     6601 acccggagag ctccttgtac gggtactcgc agcccggctg cttcgggttg atgacgacgg
     6661 cgcccgccgg gagccggtcg ggaacgaggt ggtgatcggt gacgacgact tcgacccccg
     6721 cttctctcgc gcacgccacg ccttcgatcg ccccgattcc gcagtcgacg gtgacgatca
     6781 cggaaggcgc gagctccgag aggacgcggc gcaccgtgtc gggccggaga ccgtatccgt
     6841 ccttcaatcg atggggaatg aacgagagcg cggtagcgcc ggcgcgcgcg agcgccgcct
     6901 tgagctgggc gacggcggtc acgccgtcga cgtcgtagtc gccgaagacg acgatccggc
     6961 ggccggccct cgcggccgac aggagaatgg ccgcggcttc ctcgaccccg cgcatgcgga
     7021 aaggatcgtg cagttgcgac tcgtcgggcc ggaagaacag cgcagcatct tcagcagttc
     7081 cgatcccgcg cgcgaggagg agctccgcga cgacccgcgg aagctttgcc tgacgcgcga
     7141 gggcatctgc cggatcccgc ggcgaggtcg accgcaggcg ggcgacgagg tcggaaatga
     7201 cggcgcgaat cggttccatg ggcacgagtc tcgccgggag ccgggacaga agatgtccgc
     7261 gcggcgggcg acctatccga ccggctgcga ttcgaaccgt cccatcgccc ggaacttgcg
     7321 gtagcggcgc tcgcgccgct cctcggtcgt caggtcggag acctcgcgca ggtgccgttc
     7381 gatcgcttct cccagcgccg cggcggccga ggtcggatcg gcgtgcgcgc ctcccggagg
     7441 ctcggggacg atctcgtcga tgattccgaa ttccgcgaga tcccccgccg tcacgcgcag
     7501 cgccgccgcg gcttccttct tgcgcgacgc gtccttccag aggatcgcgg cgcacccctc
     7561 cggcgagatg acggaataga tcgcgtgctc gagcatcagg atccggtctc cgacgctcaa
     7621 cgcgagggcg ccgcccgagc caccttcgcc ggtgaccgcg acgatgatcg gcacccggaa
     7681 tccggccatc gaacgcaggt tgaccgcgat cgcctcggcc tggccgcgct cctcggcgtc
     7741 gattcccgga aaggcgccgg cggtgtcaac gaacgagata atcgggcgct cgaacttctc
     7801 ggcgagctgc atgacccgga gcgccttgcg gaatccctcg ggccgaggca ttccgaagtt
     7861 gcggcggatc ttttccttgg tgtcgcgtcc cttctggtga ccgacgatcg caacgggccg
     7921 gccgcgaaac cgcgcgagcc cggcgacgac cgcggcgtcc tcggcgaact tgcggtcgcc
     7981 gtgcagctcg acgaaatcgg tcatgagcgc cccgatgtag tcgagcgtgt agggccgcgt
     8041 cggatggcgc gcgaccaggg tcttctgcca tggggtcagg cccgcgtaga tctcgcggga
     8101 ggtccggtcg agcttctccc gcagccgctc gatttccttg cggtgcgcgg cgtcgtcggg
     8161 gagggccgag agctcttcga tcctctgccg gagccgcagg atcggctctt cgaactggcc
     8221 gtcttcgccg ttcatttcgt tggattgtag atcgtcgcgc tccctggctg gggatctggc
     8281 tgacgccaac ggcgctcggc tcggcaagcc ggcctcgacc gagcgcctga tccctcgctg
     8341 cggtcgcgct cccgcggcgc aaggctcctc cctcgattac ctcgggagac tcctcgcgcc
     8401 gcgaaccgag agctaccggg tcctcggggc atctacatca ggtcctgcgg gtcgacgtcg
     8461 acgccgatct gggtccccga cggaggccgc tccgggatcg cctcgccgag catcgaaagg
     8521 acgacggacc gctccgccgc gcgcacgagg atctggaagc gccacttccc ctggaggcgt
     8581 tcgagcggcg ccggggcggg ccccgaaagg cgcaccccct ttccgccaga gcccttgctt
     8641 cccgaggccg acagtgcccg cccgatctcc gccgcgagct cctcggcctt ttcgcggtcg
     8701 gccgaggaga cgagcaccga ggcgagctcg gcgaacggcg ggtagaagaa cgcccggcgg
     8761 aactcgagct cctgctccgc gaagccgtcc acgtcgtgca ccgccgctcg ccggatcgcc
     8821 ggatggtcgg gatggaacgt ctggacgtgc acggtgcccg gacggttccc gcgccccgcg
     8881 cggccggcga cctgcgcgag cagctggaag gtcttctccg cagcccggaa gtcggggaag
     8941 ttcaggatcg agtcggcgga caggacgccg acggcggtga cgttcgggaa gtcatgcccc
     9001 ttcgcgacca tctgcgtgcc gatcaggcag cgcgcgcgcc cggagagcac gtcgtcgacg
     9061 accgcctcga cgccgcgccg acgcgccgcg tcacggtcga ggatcgtcca gggcacctcg
     9121 gggaagagga cggcgaatcg ttccgcgacc cgctcggtgc cggctccgat cgcctcgagg
     9181 agcgcaccac cgcactgcgg gcagcgcaac ggccgcggca cccgctcgcc gcagtagtgg
     9241 cagacgaggc gccggccccg atcgtgaacg gtgcgcgaca cactgcagcg cgagcagcgg
     9301 aagtcgtggc cgcagtcgcg gcagaggaga aacggtgcaa agccgcgtcg gggctggagg
     9361 aggatcgcct gctcgccgcg ggaaaatacc tccgtgagcc ggttgacgag ggcgaccgag
     9421 aagagcgcca cccccttttc ttcgggcgtc gcgcgctcgc ggcggaggtc gacgatcgcg
     9481 acctgcggga ggggacggga ctcgacgcgc gacgccaggc gcagcgacgt catgcggccc
     9541 acgctcgccg agtagtacgc ttccatggac ggcgtcgcgg atcccatgag gagagcggcg
     9601 ccgttcgctt ttgcgcggat cgccgcgacg tcacgggcgt cgtagcgagg gttctcgcgc
     9661 tgtttgtagg aagcgtcgtg ttcctcgtcg acgacgaaga ttccgggatc ggcgaccgga
     9721 gcgaaagcgg ccgaccgcgg accgatcacg accggcgctt cccccgagcg gacccgatcc
     9781 caggcgcgcg cgcgctcctt ctcgctgagc gccgaatgga gaacggcggc gcggtctccg
     9841 aactgccgct tgagctgacg cgcgaacacc ggcgtgagcg cgatctccgg gacgagccac
     9901 acggcgccgc gcccgagctc gagcgccgat gcgatcgcgc gcaggtaaac ctcggtcttc
     9961 ccgctccccg tgaccccctg gaggagggcg gcgcgcccgt gccggctgcg gatcgactcc
    10021 cgaacggtct cgagggcacg cgcctgctct tcggtcagca cgacgtccgg ccgccccggc
    10081 ggcgccaccg gcgagaggac cggctcctcg cgctccaact gctcgaacgt cgcgaggacc
    10141 cctttcgaag cgagcgatcg caggaccccg ggacctgctc ccgtcgccac ccggatctcc
    10201 cggcccgtcg ccggccggcc gagcgactcg aggtactcga atacctccct cccgcggcgg
    10261 ctgcgcgcga gccgctcctc gcgttcggcg acatcgagcc ggccgggcag ataggcgagc
    10321 tcggggcgcc gggcggcgcg gcgatcctgg gacgcagcgc gcacccatcc gcgctcctcg
    10381 agaaggcgca ggacctcgcg gcgcgggcgc gccggccgcg gcagctccga gagcgggacg
    10441 tgccgcccgt cggcgatcgc gtcgaggaag gcgcgctctt ccccttgcga ccgcccggcg
    10501 ccgagcgccc ccttctccgt gatccggtag cgcaccgcgc cggcggcggg gagccgcgcc
    10561 gggagcgcgc tcttcaggac ctcaccctcc gccgcgaaga accgttgcgc gacccgggcc
    10621 gcggtctcga gaagttcggg cggacagacg ggttcctcgt ccaggacgtc gaggatgtcg
    10681 cggagcgacg catcgtgggc gccgcccccg gcgtcctcgc ggtcgggaac gacgacgccg
    10741 gtcaggacac gttctccgaa aggcacgcgc acgcgcatgc ccggcgccgg gacgtcgagg
    10801 tgctcgggca cgcggtatgt aaagttgcgc cggatgggga ccggcagggc aaccgagacc
    10861 gtgcgcacga tcccgactct actttggact cgacccgaac ccggacctac gtctgcgccg
    10921 aggaaacccc ttcgtccccc agcgcgtcgt cgcgcacgaa aacgtatgac cgcttgagcg
    10981 ggtcccaccg gtagcggcgg gtgttctcgt cctcgtcgag gtccggggtg atcacgatcg
    11041 aatcgcggtc gaccgagagg tctccgtccg cgagcgagac gtcacgttcg atgaaggtgc
    11101 cggggccgaa gccggcgccc tccttccccg gaaacgccac tacggagaac cagggcgagt
    11161 cccgatcgga gtcgcgcttg acgaacgcga gcacgaagtc gaggatgccg tcgtcgttga
    11221 cgtccccccg aagaaagtac gggtgataga cggtgtagag ctttccgagg tcgtcggact
    11281 cgctcgggcg gcgatcgtcg tcggtcgcca tgcgcaggtc gcggttggcg cgcagaaaag
    11341 cctcgacggc ctccttctcc tccggccgga acgagtaacc ggaaccggcc gggggcgcgg
    11401 gcggtcgcgc gacgcggtcg gagttcgcaa tccgcggggc ggcgggcgga cgccgacagc
    11461 cgagcagccc ggcgaggaga gccactccgc aggcgagcgt catcgcgccg agccgcgaag
    11521 cgcgaccacg ccgcagttca cattcttccg gaaggccgag gttgtcgaag tccatctcgc
    11581 gcaggaccag tcattgtacg gcacggattc gcgcggctcg agccggacgt tctccgcgcc
    11641 ggagggcttc tgacctctca cgtcatcggt tcgaacagtg ctagtgttgc agtccgcgtg
    11701 cgatcgaagc cttcggagcg agacctctga gacaggaaaa agacagcctg ggggtgcgcg
    11761 agatccccga cgaggcttac tggggcgccc agacgtcccg cgcgatcgag aactacccga
    11821 tttcggggga gcgtgcgcac cccgagatga tccgcgccta cgcacgcatc aagaaggcgt
    11881 gcgccgagac gaacctcgag atggggggtc tccccgccga gaaggtgcgc gcgatcgttc
    11941 aggcgtgcga cgaggtgctc gccggccggc acttcgatca gttcgtcgtc gacgtctacc
    12001 aggccggggc ggggacctcg ttccacatga acgtcaacga ggtcatcgcc gggcgcgctg
    12061 ccgagctcgc cggtgcctcg cgcggcgatc gcaaggcgat caaccccaac gacgacgtca
    12121 acctcggcca gtcgacgaac gataccttcc ccaccgctct ccacatggct gcgatcgcgg
    12181 tcgggcgggt gctcgtcgcg gaggtcgaga aactcgccgc gtccttcgaa aggaagggga
    12241 aggagttcga cggaatcgtc aagtcgggcc gcacccacct gatggacgcg gtgcccgtca
    12301 cgctcgggca ggagttccgc gcgttcgcct ctgcgatctc gcaccggcgg gaaggactga
    12361 cgcgagcgct cgaccggctg cgagagttgc ccctgggtgg gacggcgacc gggaccggga
    12421 tcaacaccgc ggccggcttc cgcgaaaagg cgatcgagaa gctcgcgaga ctggcgggtg
    12481 aagcgttctc gccggcgacc gacgcccggg agggcctcca gagccgagcc gcggccggtg
    12541 actattcggc agcgctccgc tcctccgccg tcgagctcgg gcggatcgcc aacgacctgc
    12601 ggctcctcgc ctccgggccg gcgacgggcc tggacgaggt ccggctgccg gccgtccagc
    12661 cggggtcgtc gatcatgccg ggcaaggtga acccgtcgct cctcgaatgc ctcaacatgc
    12721 tctgctacca gatcgtcggg ttcgacaccg cgaacctgct cgccgtcggt ggcggccagc
    12781 tcgacctgaa cgtcatgatg ccgctcctcg cgtacaacct gacgagggga ccacagcttc
    12841 tcatcaattt tctgccggtc gtgaggcagc gatgcatcga cgggatcacc gcgaacgtgg
    12901 aaaagtgccg cctctacctg gaggagtccc cgtcggtcgt gaccgcgctg accccgaaga
    12961 tcgggtacgc gacggcagcg gaagttttca aggaggcagt cgcgcgggga gtcctcgtca
    13021 aacaggtgct tctcgagaag aagatcctga ccgaagatga gctcgcggcg gcgatgacga
    13081 aagaggcgct cctgggtccc ctcgagtaat ccggttttcg attcgattcc ggttgagcga
    13141 aatctcgact ccgaggagga tggacagatg acctggccgc aggactggca gaccaacgcc
    13201 acgttccttc tccggtgggt gcaccttctc gcaggcatta cctggatcgg cctgctgtac
    13261 tggttcaacc tcgtcaacgt gaacttccag aaggaaatcg acgctgcagt gaaaggcaag
    13321 gtcaacccgc cgctgctctc gcggtctctc tggtggttcc gctggggagc gatagtgacc
    13381 tggctgaccg gcgccgctta ctacggaatc cttctcggta ccgagaacat cggcgc
//
LOCUS       DASWAV010000458         1535 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U2665
            k141_383190, whole genome shotgun sequence.
ACCESSION   DASWAV010000458 DASWAV010000000
VERSION     DASWAV010000458.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960316
            Sequence Read Archive: SRR10260007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 1535)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1535)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:47:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,929
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1535
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_383190"
                     /isolate="SMAG_U2665"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.36444 N 132.31889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            148..888
                     /locus_tag="VF958_09565"
     CDS             148..888
                     /locus_tag="VF958_09565"
                     /inference="COORDINATES: protein motif:HMM:NF024258.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HEX9149395.1"
                     /translation="MRYLLLSDIHSNDEALAAVLARARGRRFDRVVILGDFVGYGAEP
                     NAVLDRLRRIRKEKFVIRGNHDKVVCGLDDGELFNPVALQAARWTEKELTPENREFLE
                     ALPLGPRGVEGAFEICHGSPRDEDAYIFSDRDAFVNFRGFDSRVCFFGHSHIPSIFTL
                     EPHGIKVEVVYGKHVRQLEEGRRYLINPGSVGQPRDRNPAASYAIYDAGKKRVSFDRV
                     PYDAERTRQKIHRAGLPGMLGDRLLVGA"
     gene            complement(1290..>1535)
                     /locus_tag="VF958_09570"
     CDS             complement(1290..>1535)
                     /locus_tag="VF958_09570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9149396.1"
                     /translation="PTRFYSADRGEEKQKIEKAWKDVYALPDSVSAPISDEAMLRYGV
                     SATPTFVLVNRKGTVVRYSPTRMTEDRLSSAIEDLLR"
BASE COUNT          236 a          535 c          502 g          262 t
ORIGIN      
        1 gccgagcgcg agcctttcta tagaatgggg tcggtctccg tcgctccgag cggagagtcg
       61 atcgaagagg cggcggaccg cgtgctcgac gctctctcgg cccggaaccg ctggaagccg
      121 gctccgtagc tcacgatcaa gccacccatg cgctacctgc tcctctcgga catccattcc
      181 aacgacgagg ccctcgcggc cgtgctcgcg cgggcgcggg gccggaggtt cgaccgcgtc
      241 gtcatcctcg gcgatttcgt cggttacggg gccgagccga acgccgtcct ggaccgcctg
      301 cgccggatcc gcaaggagaa gttcgtcatt cgcgggaacc acgacaaggt cgtgtgcgga
      361 ttggacgacg gggagctgtt caacccggtc gccctccagg cggcccgctg gacggagaag
      421 gagctgacgc ccgagaaccg cgaattcctc gaagcgctgc cgctcgggcc gcgcggcgtc
      481 gagggcgcgt tcgagatctg ccacggctcc ccccgcgacg aggacgccta catcttctcc
      541 gaccgggacg cgttcgtgaa ttttcgcgga ttcgattcgc gtgtctgctt cttcggccac
      601 agccacatcc cgtccatctt cacgctcgag ccgcacggca tcaaagtcga agtcgtttac
      661 gggaagcacg tccggcagct cgaggagggc cgccgctacc tgatcaaccc cggctccgtc
      721 ggccagccgc gcgaccgcaa cccggccgcc tcctacgcga tctacgacgc cgggaagaag
      781 cgcgtttcct tcgatcgcgt gccctacgac gccgagcgaa cacgccagaa gatccaccgg
      841 gcgggcctgc cggggatgct cggcgatcgc ctgctcgtgg gcgcgtaacc gtcgcgaagc
      901 gctgcgggag agatctgcgc ctttggcgcc ccggctcgcg gctcggccgg atgcggcctc
      961 acacgtcgct acgcgctgcg ggcgggatct gcgcctgcgg cgcaccggcg atcggctcgg
     1021 ccggatgcgg cctcgaccgc tcgcctggtc ccgtcctcgc gcttcgctcc cgcggcgcgc
     1081 gcctcctccc tcggttactt cgggagacta cgcgcgccgc gaccgggggc acttgaggag
     1141 atgtcatcct gaaaccagcg acgctctccc ctctgtcatc ctgagcgaag cgaaggatct
     1201 gctctcgtgt ttccaacatt ccggagcaga tccctcgtcg cgggcaaacg ctccttcggg
     1261 atcgggaact caggacaccc ggaggttgct cagcgcagca ggtcttcgat ggcggaggag
     1321 aggcggtctt ccgtcattct cgtcggcgag tagcggacga ccgtcccctt cctgttgacc
     1381 aggacgaacg tcggcgtcgc ggagacgccg taacggagca tcgcttcgtc gctgatcggg
     1441 gccgaaacgg aatcgggcag ggcatagacg tccttccagg ccttctcgat cttctgcttc
     1501 tcttcgccgc ggtcggcgga atagaagcgt gtcgg
//
LOCUS       DASWAV010000459         4598 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U2665
            k141_265644, whole genome shotgun sequence.
ACCESSION   DASWAV010000459 DASWAV010000000
VERSION     DASWAV010000459.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960316
            Sequence Read Archive: SRR10260007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 4598)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4598)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:47:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,929
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4598
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_265644"
                     /isolate="SMAG_U2665"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.36444 N 132.31889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            296..1807
                     /locus_tag="VF958_09575"
     CDS             296..1807
                     /locus_tag="VF958_09575"
                     /inference="COORDINATES: protein motif:HMM:NF019334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing serine/threonine-protein
                     kinase"
                     /protein_id="HEX9149397.1"
                     /translation="MTLRGLFGRKKKDDTGPGPETEAASPADGKPTGRIGKYEIVEKI
                     GAGGFGTVTKGWDPLIRRHVAIKTCEVGNKEIRNRFFREAQLAGGLQHPNITMVYEFG
                     FEGDVPFMVQEFLSGEDLDRIIKGDAALSLPDKLRILIGIAFGLEYAHKAGVMHRDIK
                     PANVRVLDNQFVKIMDFGIAKTIEAADDITQTGITVGSSSYMSPEQIAGDSLDCRTDI
                     FSFGVLAYELLSGRKPFSNDNLFMLLEQIVKEEPRPLSEMAPDTPPALVAIVERAMKK
                     KPEDRFPSARELRDALVAVQHEVDAASGTSDSLEIPLPDDEAGRLAALERLEILDTEP
                     EREFDDLAVLAAQVCATPLALISFVDRDREWFKSKVGISESSFPRMLAFCAHAIAGRD
                     VFVVPDAAADPRFSANPLVTRKPGVRFYAAAPLLTPEGYAVGALCVIDLAPRELVPGQ
                     IEALKTLARQVMAQLELRRRRRSDREQSGEKLMLEVAGLADREPSLIPPPKPQ"
     gene            1804..2214
                     /locus_tag="VF958_09580"
     CDS             1804..2214
                     /locus_tag="VF958_09580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012234363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX9149398.1"
                     /translation="MTKASNILIVDDDEGTLASLGGLLELEGYSVEKARNGREALDRL
                     GTATEAPGLILLDLNMPVMDGWQFLTERAKDAVARAAPVVLLSGLPYIPNAEGVADFL
                     TKPVNAARLLACVRRFCGEPNGGISLTARSEPSP"
     gene            complement(2218..2973)
                     /locus_tag="VF958_09585"
     CDS             complement(2218..2973)
                     /locus_tag="VF958_09585"
                     /EC_number="2.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011699706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004719 - protein-L-isoaspartate
                     (D-aspartate) O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate(D-aspartate)
                     O-methyltransferase"
                     /protein_id="HEX9149399.1"
                     /translation="MKYACVVLSLLVISASGCKGPVADTTRVTATPSETDAERRARMV
                     ETQIVARGVRDPRVLAAMRKVPRHLFVDPAQRAQAYEDHPLPIPGNQTISQPYIVALM
                     TELLDVRPGERVLEIGTGSGYQSAVLAELAKEVYTIEIVPELARSAASRLKDLQYRNI
                     TVREGDGYGGWPDQAPFDAIIVTAAPERIPQPLIEQLAPGGVMVIPVGGFFQELKVFR
                     KSAEGRVTEKDILPVRFVPMTGEVEKTPTAADQ"
     gene            complement(2970..>4598)
                     /locus_tag="VF958_09590"
     CDS             complement(2970..>4598)
                     /locus_tag="VF958_09590"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF019581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HEX9149400.1"
                     /translation="GTAPPPTTTLLLEDGTVYVSAEAKARKASIVLRDGKVEFVGDAA
                     RARTLTRGARRMNLTGAFVFPGWADAHGHLLGLGKSLEIADMRGAASASEAAKRVGDL
                     AAKLPAGAGVEARGWAQNLWPGKAFTDARELDAVAGGRPVAARRVDGHAVWVNSVALE
                     RAGIRSDTKDPEGGRILRRPDGSPSGVLVDNAADLVDNVVPAPSAADVERRFLAAMRA
                     CARAGLTEVQDASGYGSDEIAALERLAARRELPIRVYATVSPEAASLAHFFSKGIRVG
                     SGDDFLTVRAIKAYADGALGSRGAALLADYSDEPGKQGLLVTPPERLEEVARQARDHG
                     WQLWIHAIGDRGNRVALDALQKAGAAGGTATRPRIEHAQVVAAEDIPRFGRENVIASV
                     QPTHATSDMGWAEARLGPERIRGAYAWRSLKNAGARLAGGSDFPVESEKPLLGFYAAI
                     TRKDLSGRPPGGWRSSEKLSRSEALALFTSDAAWASFEEETRGKIAPGYAGDLTVFRR
                     DPMTAPEQEIPTIPIAMTIVGGRVFEPTRAEPPK"
BASE COUNT          730 a         1607 c         1528 g          733 t
ORIGIN      
        1 tccgattcga gacgagagcc cgtgcaacgg cttggtcgag ggtgagcagt ggcgcgggcg
       61 tggcgtcttc cgcacgagca ggactgaccc aggcggaggc ccagaccgcg aggacgatcg
      121 cagtcggaag acccccgacg gcgcgcaacc ctcggctgga gggaaatacc cggcttttgc
      181 ttgcctgcgc ggccagcgcc gcgtactgca aatcccctct ccgctttcga gtgacgctat
      241 cacgcggtcc gcgctcctga ttcaggcacg cttcttgttc ttacaatgcg ccgcaatgac
      301 gctccggggc ctcttcggcc gaaaaaagaa agacgacacg ggtcccgggc cggagaccga
      361 agccgcttcc ccggcggacg ggaagccgac cgggcggatc ggaaagtacg agatcgtcga
      421 gaagatcggg gcgggcggct tcgggaccgt caccaagggg tgggaccccc tcattcgccg
      481 ccacgtggcg atcaagacct gcgaggtggg aaacaaggag atccgcaacc ggttcttccg
      541 ggaggcgcag ctcgccgggg gtttgcagca cccgaacatc accatggtct acgagttcgg
      601 cttcgaaggg gacgtccctt tcatggtcca ggagttcctc tcgggcgaag atctcgaccg
      661 gatcatcaaa ggcgacgcgg cgctctcgct cccggacaag ctccgaatcc tgatcgggat
      721 cgccttcgga ctcgagtacg cccacaaggc cggcgtcatg caccgcgaca tcaagccagc
      781 gaacgtgcgt gtgctcgaca accaattcgt caagatcatg gacttcggca tcgcaaagac
      841 catcgaagct gcggacgaca tcacgcagac cgggatcacg gtcggatcgt cttcctacat
      901 gtcacccgag cagatcgccg gcgattcgct cgactgccgc accgatatct tctcgttcgg
      961 ggtgctcgcc tatgagctcc tctccggccg caagccgttc agcaacgaca acctcttcat
     1021 gctcctcgag cagatcgtga aggaggagcc gcgcccgctc tcggagatgg cgccggacac
     1081 gccgcccgct ctcgtggcga tcgtcgaacg cgcgatgaaa aagaagcccg aggaccgctt
     1141 cccctccgcc cgcgagctcc gcgacgcgct cgtcgccgtc cagcatgagg tcgatgcagc
     1201 gagtgggacg tccgactccc tcgagatccc gctccccgac gacgaggcgg gccgcctcgc
     1261 ggcgctggaa aggctcgaga tcctcgacac ggagcccgaa cgtgagttcg acgacctcgc
     1321 ggttctcgcg gcgcaggtct gcgccacccc cctggcgctg atctcgttcg tggatcgcga
     1381 ccgcgaatgg ttcaagtcga aggtgggaat ctccgagagt tccttcccgc gaatgctcgc
     1441 gttctgcgcc cacgcgatcg ccggacgcga cgttttcgtg gtgcccgacg cggccgccga
     1501 cccccgcttc tcggccaacc ctctcgtcac gcggaagccg ggtgtgcgct tttacgcagc
     1561 cgcgccgctg ctgaccccgg agggatacgc ggtcggagcg ctctgcgtca tcgatctcgc
     1621 gccgcgggag ctcgtgccgg ggcagatcga ggccctgaag acgctggcga gacaggtgat
     1681 ggcccagctc gagctccggc gtcgccgccg ttccgaccgg gaacagtccg gtgagaagct
     1741 gatgctcgag gtggcggggc tcgcggaccg cgagccttct ctcattcctc cgcccaagcc
     1801 gcaatgacga aggcgagcaa catcctgatc gtggacgacg acgagggaac gctcgcctcg
     1861 ctcggcggcc tgctcgagct cgagggctac tcggtcgaga aggcacgcaa cggacgggaa
     1921 gctctcgaca ggctcggtac ggcgaccgag gcgcccggtt tgatcctgct cgacttgaat
     1981 atgccggtga tggatggctg gcaattcctg acggaacgcg cgaaggacgc cgtcgcgcga
     2041 gcagcgccgg tcgttctcct ctccggcctc ccctacatcc cgaacgcgga gggggtcgcg
     2101 gacttcctca ccaagccggt caacgcggcg cgcctgctcg cctgcgtccg gcgcttctgc
     2161 ggcgaaccga acgggggaat ctctctcacg gcgcgatcgg agccgagccc ctagccttta
     2221 ctgatccgcc gccgtcggcg tcttctcgac ctcgccggtc atcggaacga acctcacggg
     2281 gaggatgtcc ttctcggtca cgcgcccctc ggcggacttg cggaagacct tgagctcctg
     2341 gaagaaacct ccgaccggga tgaccatcac gccgcccggc gcaagctgct cgatcagtgg
     2401 ctgcgggatc cgctcgggcg ccgcggtcac gatgatcgcg tcgaacgggg cctggtcggg
     2461 ccaacccccg tatccgtccc cctcgcggac ggtgatgttc cggtactgca gatccttcag
     2521 gcgcgaagcg gccgagcgcg cgagctcggg gacgatctcg atcgtgtaga cctctttcgc
     2581 gagctccgcc aggacggcgc tctgataacc cgacccggtg ccgatctcga gcacgcgctc
     2641 ccccggccgg acgtcgagga gctccgtcat gagagccacg atgtacggct gcgagatcgt
     2701 ctggttaccc ggaatcggaa gcgggtgatc ctcgtatgcc tgcgcacgct gagcgggatc
     2761 gacgaagagg tgacggggaa ccttgcgcat cgctgcaagg actcgtggat cgcgcactcc
     2821 ccgcgcgacg atctgcgtct cgaccatccg cgcgcggcgt tcggcgtcgg tttccgacgg
     2881 ggttgccgtc acccgggtcg tgtccgcgac cgggcctttg cagccggaag ccgagataac
     2941 gaggagcgac agaacaacgc acgcatactt cacttcggcg gctccgctcg cgtcggctcg
     3001 aagacccggc caccgacgat cgtcatcgcg atcggaatcg tcggaatctc ctgctcgggg
     3061 gcggtcatgg gatcgcgccg gaagacggtg aggtcgccgg catatccggg cgcaatcttt
     3121 ccacgggtct cctcctcgaa ggacgcccac gccgcgtcgg aggtgaagag cgcgagcgct
     3181 tcggagcgcg agagcttctc ggatgagcgc cagccgccgg gcgggcggcc ggaaaggtcc
     3241 tttcgcgtga tcgcggcata gaagccgagg agcggctttt ccgactcgac gggaaagtcg
     3301 cttccccccg cgagccgggc gccggcattc ttcaggctcc tccaggcgta cgcgccccgg
     3361 atcctctcgg ggcccaggcg ggcctcggcc caccccatgt cggaggtcgc gtgcgtcggt
     3421 tgaacggatg cgatcacgtt ctcgcgtccg aagcggggga tgtcctcggc ggcgaccacc
     3481 tgcgcgtgtt cgattcgcgg ccgggtggcc gttcctccgg cggcgccagc tttctgcaac
     3541 gcgtccaggg ccacgcggtt cccgcggtcg ccaatcgcgt ggatccacag ttgccatccg
     3601 tggtcgcgcg cctgccgcgc gacctcttcc agccgctccg gaggcgtcac gaggagtccc
     3661 tgcttcccgg gttcgtcgga gtagtcggcg agaagcgccg cgccgcggct gcccagcgcg
     3721 ccgtcggcgt acgccttgat cgcgcggacg gtgaggaagt cgtcgccgct ccccacccgg
     3781 attcccttcg agaagaagtg agcgagcgac gccgcctccg gggacacggt cgcatagacc
     3841 cggatcggaa gctcgcgccg cgcggcgagc ctctccagcg ccgcgatctc gtcgctcccg
     3901 tagccggagg cgtcctgcac ttccgtcaag cccgctctcg cgcacgcgcg catcgcggcg
     3961 aggaatcgac gctcgacgtc cgcggcactc ggcgccggaa cgacgttgtc gacgaggtcc
     4021 gccgcgttgt cgacgagaac tccggaaggc gaaccgtcgg ggcggcgcag gatccggccg
     4081 ccctccggat ccttcgtatc ggagcggatt cccgcccgct cgagagcgac ggagttgacc
     4141 cacaccgcgt gcccgtccac gcggcgcgcc gcgaccggcc gcccgcccgc gacggcatcg
     4201 agctcgcggg cgtcggtaaa ggctttcccg ggccacaggt tctgagccca tccgcgcgct
     4261 tccaccccgg cgcctgccgg cagtttcgcc gcgaggtcgc cgacgcgctt cgccgcctcg
     4321 gacgcgctcg ccgcgccgcg catgtctgca atctcgaggg acttgccgag accgaggagg
     4381 tgcccgtggg cgtccgccca tcccgggaag acgaacgcgc ccgtgagatt cattcgcctt
     4441 gctccgcgcg tgagggtccg ggctcgagcg gcgtccccga cgaactcgac cttcccgtcc
     4501 cggaggacga tcgacgcctt gcgggccttc gcttccgccg agacgtagac ggtgccgtct
     4561 tccaggagca gcgtcgtggt cgggggcggg gccgttcc
//
LOCUS       DASWAV010000460         1673 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U2665
            k141_664873, whole genome shotgun sequence.
ACCESSION   DASWAV010000460 DASWAV010000000
VERSION     DASWAV010000460.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960316
            Sequence Read Archive: SRR10260007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 1673)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1673)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:47:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,929
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1673
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_664873"
                     /isolate="SMAG_U2665"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.36444 N 132.31889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(158..406)
                     /gene="purS"
                     /locus_tag="VF958_09595"
     CDS             complement(158..406)
                     /gene="purS"
                     /locus_tag="VF958_09595"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007671079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurS"
                     /protein_id="HEX9149401.1"
                     /translation="MKVEVRVYPKEGVLDPQGRAIASALGRLGHRSVQDVRAGKVFFI
                     DVAGTDPVAVEAEARKMAEELLANTVIEGYDVRVAAEP"
     gene            complement(403..1173)
                     /locus_tag="VF958_09600"
     CDS             complement(403..1173)
                     /locus_tag="VF958_09600"
                     /inference="COORDINATES: protein motif:HMM:NF024118.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEX9149402.1"
                     /translation="MRVSVLASGSGGNACVVESGRTRVLVDAGLSSKEIEKRLHARGV
                     EPETIAAIFLTHEHGDHSIGALTYAARFGCPIFATAGTAAALGLEGNLFSPFVRMESC
                     REGRVGDIGFRPLATPHDSNESVAYAFEADDARMVVASDLGHVQREFLDFLRGATTLL
                     LEFNHDEDMLRDGPYVWALKRRIAGGFGHLSNRQSADALRATAGERLRRVIATHLSRT
                     NNTPALALATLGSALELAQSAATYGVADQFDGFETFDA"
     gene            complement(1258..>1673)
                     /gene="dut"
                     /locus_tag="VF958_09605"
     CDS             complement(1258..>1673)
                     /gene="dut"
                     /locus_tag="VF958_09605"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019995077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004170 - dUTP diphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006226 - dUMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0046081 - dUTP catabolic process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HEX9149403.1"
                     /translation="GEGLDLPVYATEGSAGCDLRAAIQAELTITPGGRALIPTGIAVA
                     IPPGYEGQVRMRSGLAHDKGLALLNAPGTVDSDYRGELRIIIANLGSEPATIHRGDRI
                     AQLVFAPVARARFERVSELPPTTRNTGGFGSTGHS"
BASE COUNT          278 a          573 c          575 g          247 t
ORIGIN      
        1 ccgggcagga tgacgatgtc ggcgcccttc aagtccgttt cccggtgcca gacgtagacg
       61 gcgtcgcacc cgaggagctc ggtcgcgacg tagtggcagt cctggtcgca gttggagccg
      121 gggaagacga cgatcccgaa cttgggcttg ccggcggtca cggctccgcc gcgacgcgca
      181 cgtcgtagcc ctcgatcacg gtgttcgcga ggagctcttc cgccatcttt cgcgcctcgg
      241 cctcgacggc gaccggatcc gttcccgcca cgtcgatgaa gaacaccttg ccggcccgga
      301 cgtcctggac cgaccgatgt ccgaggcggc cgagcgccga cgcgatggcc cgaccctgcg
      361 ggtcgagaac gccctccttc gggtagacgc ggacttcgac cttcatgcgt cgaacgtctc
      421 gaagccgtcg aactggtccg ccactccgta tgtcgccgcg ctctgcgcga gctcgagcgc
      481 tgaaccgagc gtcgcgagcg ccagcgccgg cgtgttgttc gtacgcgaga ggtgcgtggc
      541 gatgacccgt ctcagtcgct caccggcggt cgcccgcaac gcgtccgcgg actggcggtt
      601 cgagagatgc ccgaaccccc ccgcgatccg gcgcttgagc gcccacacgt acggcccatc
      661 gcggagcatg tcctcgtcgt ggttgaactc gaggaggagg gtcgtcgcgc cgcgcaggaa
      721 gtcgaggaac tcccgctgca cgtgtccgag atccgacgcg acgaccatcc gtgcgtcgtc
      781 cgcctcgaac gcgtaggcga cggactcgtt ggagtcgtga ggcgtggcga gcggacggaa
      841 gccgatgtcg ccgactcgcc cctcccggca cgactccatg cgcacgaagg gcgagaaaag
      901 atttccctcg aggccgagcg ccgcagctgt gccggcggtc gcgaagatcg ggcagccgaa
      961 acgcgccgcg tacgtcagcg cgccgatcga gtggtcgccg tgctcgtggg tgaggaagat
     1021 cgcggcgatc gtctccggct cgaccccgcg cgcgtgaagc ctcttctcga tttccttcga
     1081 cgaaagtccg gcgtcgacga gcacgcgagt ccggcccgac tcgacgacac aggcgtttcc
     1141 acccgagccg gaggcgagga cggagacgcg cacgattgaa gagtagcaga gggcacaggg
     1201 acaaaagatg tccgagccgg gcgggaaacg gaaaacggga aaacgggaaa cgagggatca
     1261 ggagtgaccg gtcgacccga accctcccgt gttgcgcgtc gtcgggggca actcggagac
     1321 gcgctcgaag cgcgcccgcg ccaccggcgc gaatacgagc tgagcgatgc gatcaccgcg
     1381 atggatcgtc gcgggctcgg acccgaggtt cgcaatgatg atgcgaagct ctccccggta
     1441 gtccgagtcc acggtccccg gcgcgttcag gagcgcgagc cccttgtcgt gcgcgaggcc
     1501 gctcctcatc cgcacctgtc cctcgtagcc gggcgggatc gcgacggcga tcccggtggg
     1561 aatgagagcc cgccccccgg gagtgatcgt cagctccgcc tgtatcgccg cccgcaggtc
     1621 gcaacccgcc gatccttcgg tcgcgtacac cgggaggtcg agcccctcgc cgt
//
LOCUS       DASWAV010000461         2265 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U2665
            k141_186447, whole genome shotgun sequence.
ACCESSION   DASWAV010000461 DASWAV010000000
VERSION     DASWAV010000461.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960316
            Sequence Read Archive: SRR10260007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2265)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2265)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:47:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,929
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2265
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_186447"
                     /isolate="SMAG_U2665"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.36444 N 132.31889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..688
                     /locus_tag="VF958_09610"
     CDS             <1..688
                     /locus_tag="VF958_09610"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HEX9149404.1"
                     /translation="EHAFAAQQRLVADAAHELKTPIAVLLGEAQDALRAESRPEERRK
                     SLETIEATARGLAREVETLLSLARADAAMTAAFAVVDLAAVAAQSVESSRSLAAARGV
                     RLDLLPENGARVRGDRSALFHLASNLVVNAATYTDPGTVVEVVAGMHDGEAFLEVRDR
                     GPGVPAEHRRRIFDRFFRLEEARLRHPEGSGLGLAIAEQVARAHGGRVEVEDRAGGGA
                     VFRAVLPAAG"
     gene            complement(881..1195)
                     /locus_tag="VF958_09615"
     CDS             complement(881..1195)
                     /locus_tag="VF958_09615"
                     /inference="COORDINATES: protein motif:HMM:NF039822.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5666 domain-containing protein"
                     /protein_id="HEX9149405.1"
                     /translation="MKKAAFVALFSIVAAIGVLSAAPKSVHAMTISGTVSAVNQKAKT
                     FSLKDDKGKETAITWTAATRVTGGALKDGDRVNVSVFPKDGKNVATSIRIASANAMAK
                     AS"
     gene            complement(1319..1612)
                     /locus_tag="VF958_09620"
     CDS             complement(1319..1612)
                     /locus_tag="VF958_09620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9149406.1"
                     /translation="MLRVKGMRDVSPGKRLFVIEDDGEPAPVATIERVAAIAQRLLHE
                     NELPGEFQSFDVGLLALNIRARERTFFHDGIVFAALDAVNDPGKNPTVTQNAG"
     gene            1764..>2265
                     /locus_tag="VF958_09625"
     CDS             1764..>2265
                     /locus_tag="VF958_09625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013186648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choice-of-anchor B family protein"
                     /protein_id="HEX9149407.1"
                     /translation="MSRRALLALAVFLGSRSASATPIACVGGLAGSYPCNNVDLLAVV
                     PLSTMGCASGNDIWGWTDSTTGREYALMGCDNGIAFVDVSDSANPVYVGKLPTHTVAS
                     LWRGVKTMRDHALVVSEAAGHGLQVFDLRNLRAFAGTPLTFSETAHYPGFGDSHNVVA
                     DEITGFG"
BASE COUNT          365 a          752 c          745 g          403 t
ORIGIN      
        1 cgagcacgca ttcgccgcac agcaacggct cgtcgccgac gccgctcacg agctgaagac
       61 gccgatcgcg gtcctcctcg gggaggcgca ggacgcgctg cgggcggaga gccggccgga
      121 ggagcgccgg aagtctctcg agaccatcga ggcgaccgcg cgggggctcg cccgtgaggt
      181 cgagaccctg ctctcgctcg cccgcgcgga cgcggcgatg acggccgctt tcgcggtcgt
      241 ggacctggcg gcggtcgccg cgcagtcggt cgaatcttcg cggtcgctcg cagccgctcg
      301 cggcgtgcgg ctggatctgc tgcccgaaaa tggcgctcgc gtccgaggcg accgatcggc
      361 gctctttcac ctcgcgtcga acctcgtcgt caacgccgcc acctacacgg atccgggcac
      421 cgtcgtcgaa gtcgtcgcgg gaatgcacga cggcgaagcg ttcctcgagg ttcgcgatcg
      481 cggccccggg gttccggcgg aacaccgccg ccggatcttc gaccgtttct tccgtctcga
      541 agaagccagg ctccgccacc ccgaaggctc gggtctgggg ctcgcgatcg ccgagcaggt
      601 cgctcgcgcg cacggaggac gggtcgaggt ggaggatcgc gccggcggcg gcgcggtttt
      661 ccgcgccgtg ctcccggccg ctggatagcc ggggtcaggt ctctacttgc cactttgctt
      721 cgcaaagatg gcaagcgcgc cgaggcgcaa agaggaccgg ggcgactcgt gaagacctga
      781 ccccaaaaaa aagcggccgg agccgccggg ctccggccat atggaggggg gatgtgctcc
      841 gcgaagtgcg ggttcgggga ccgaaaggac ccgagctccg ctacgacgct ttcgccatcg
      901 cgttcgctga cgcgatccgg atcgaggttg cgacgttctt tccgtccttc ggaaagacgc
      961 tcacgttgac gcgatctccg tccttcaggg cgccgccggt cactcgcgtt gccgccgtcc
     1021 acgtgatcgc ggtctctttg cctttatcgt ctttcagcga gaacgtcttc gccttttgat
     1081 tgaccgcgga gaccgttccc gaaatggtca tcgcgtgcac gctcttcggc gccgcggaca
     1141 acacgccaat cgcggcgacg attgaaaata gtgcaacgaa tgcggccttc ttcatgacct
     1201 tctctccttt catcagaagc gtagagagag tgcagtgaac atcgggtgaa tgcattgtgg
     1261 cgatggggga aatgaacaga gcgtgaacgg aacccggggc gcgctgcgcc gctcggggtc
     1321 agcccgcgtt ctgagtgacg gtcggattct tcccggggtc gttgacggcg tccagggcgg
     1381 cgaagacgat tccgtcgtga aagaacgtgc gctctctcgc gcggatgttc aacgcgagca
     1441 gacccacgtc gaaagactgg aactcgccgg gaagctcgtt ctcgtggagg agacgctggg
     1501 cgatcgcggc gacgcgctcg atcgtcgcga ccggagcggg ctcgccgtcg tcttcgatga
     1561 cgaagagcct cttccccgga gagacgtcgc gcatcccctt gaccctgagc accccgaagt
     1621 cagtctatgc gaactcggga gggccaaaat ggaccgggcc acgagcgcag cgacgtcctc
     1681 gaccgcgact cctgcgtcga cggcgattca ctcatggctg atcgacgcta caatcccttc
     1741 cttcacaaga gtccgttccg tcaatgagca ggcgcgctct tctcgctctc gccgtcttcc
     1801 tcgggagccg ttcggcttcc gcgaccccca ttgcttgcgt cggcggtctc gccgggtctt
     1861 acccctgcaa caacgtcgat ctcctcgcgg tcgtgcctct gtcgacgatg ggctgcgcgt
     1921 cgggaaacga catctggggc tggaccgact ccacgaccgg ccgcgaatac gcgctgatgg
     1981 gttgcgacaa cggcatcgcg ttcgtcgacg tgagcgactc ggcgaacccg gtgtacgttg
     2041 gcaagctccc gactcacacg gtcgcctccc tctggcgggg cgtcaagacg atgcgggacc
     2101 atgcgctcgt cgtcagcgag gcggccggcc acggactcca ggttttcgac ctccggaacc
     2161 ttcgtgcctt tgccggaact ccgctgacgt tctcggaaac cgcgcattac cccggtttcg
     2221 gcgattcgca caatgtcgtc gccgacgaga tcaccgggtt cgggt
//
LOCUS       DASWAV010000462         1507 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U2665
            k141_225731, whole genome shotgun sequence.
ACCESSION   DASWAV010000462 DASWAV010000000
VERSION     DASWAV010000462.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960316
            Sequence Read Archive: SRR10260007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 1507)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1507)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:47:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,929
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1507
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_225731"
                     /isolate="SMAG_U2665"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.36444 N 132.31889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..524)
                     /locus_tag="VF958_09630"
     CDS             complement(<1..524)
                     /locus_tag="VF958_09630"
                     /inference="COORDINATES: protein motif:HMM:NF019581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX9149408.1"
                     /translation="MLGLVFAVLTATASPPATTLLLEHGTVYVSAEAKTRKASIVLRD
                     GKVEFVGDAARARTLARGARRMDLTGAFVFPGWADAHGHLLGLGKSLEIADMRGAASA
                     SEAAKRVGNLAAKLPAGAWVEARGWDQNLWPGKAFPDARELDAGAGGRPVAARRVDGH
                     AVWVNSVALERAGIR"
     gene            complement(566..>1507)
                     /locus_tag="VF958_09635"
     CDS             complement(566..>1507)
                     /locus_tag="VF958_09635"
                     /inference="COORDINATES: protein motif:HMM:NF018884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PP2C family protein-serine/threonine
                     phosphatase"
                     /protein_id="HEX9149409.1"
                     /translation="ARLNGCEKCLAVSIAVPGRRLGVLAVADKESRDGRVLDFTPGDA
                     RLLSLFANQAAAAIETTRLHRAALEKERIERELELAAAIQREILPRELPRIPGLELAG
                     ATLPTRQVGGDYFDLFPLSRGRLGFVVADVSGKGIPAALLVSTVHAAIHLQIDVSDTI
                     VELVSRIDRHLQRFSATHKFLTLFFGIVEPEASLLRYVSAGHNPALLARSSGEIERLG
                     ATGVPVGIVANGSWHEETARYDAGDLLCVYTDGFTEATNADDEEFGLERLERGLAARR
                     TLPARDLSDELFREVAEFAQGVPQYDDQTLLIVRRET"
BASE COUNT          238 a          531 c          514 g          224 t
ORIGIN      
        1 cggattcccg cccgctcgag agcgacggag ttgacccaca ccgcgtgccc gtccacgcgg
       61 cgcgccgcga ccggccgccc gcccgcgccg gcatcgagct cgcgggcgtc gggaaatgct
      121 ttcccgggcc acaggttctg atcccatccg cgcgcctcca cccaggcgcc tgccggcagt
      181 ttcgccgcga ggttgccgac gcgcttcgcc gcctcggacg cgctcgccgc accgcgcatg
      241 tctgcaatct cgagggactt gccgagaccg aggaggtgcc catgggcgtc cgcccatccc
      301 gggaagacga acgcgcccgt gagatccatt cgccttgctc cgcgcgcgag ggtccgggcg
      361 cgagcggcgt ccccgacgaa ctcgaccttg ccgtcccgta ggacgatcga cgccttgcgg
      421 gtcttcgctt ccgccgagac gtagacggta ccgtgttcca ggagcagcgt cgtggccggg
      481 ggcgaggccg ttgctgtcag tacggcaaag acgagaccca acatgccggc gagtttagcg
      541 cgcggcacgc gcttcgtccc acctcttacg tctcgcgtct cacgatcagg agggtctgat
      601 cgtcgtactg cgggacgccc tgcgcaaact ctgccacctc gcgaaaaagc tcgtcggaga
      661 ggtcgcgagc aggcagtgtg cgccgcgcgg cgagcccgcg ctcgagccgt tcgaggccga
      721 actcctcgtc atcggcgttc gtggcctccg tgaagccgtc ggtgtacacg cagagcaggt
      781 cgccggcgtc gtaccgcgcc gtctcctcgt gccacgagcc gttcgccacg atccccacgg
      841 ggacgccggt cgcgccgagc cgctcgatct cgccggacga tcgcgcgagc agtgccggat
      901 tgtgcccggc cgagacgtat ctcaggagcg acgcttcggg ctcgacgatg ccgaagaaga
      961 gcgtcaggaa cttgtgagtc gccgagaacc gctgcaggtg ccggtcgatc cgggacacga
     1021 gctccacgat cgtgtcggat acatcgatct gaaggtgaat tgcggcgtga acggtcgaaa
     1081 cgaggagcgc cgcgggaatc ccctttccgg aaacgtccgc cacgacgaag ccgagccttc
     1141 cgcgcgacag cgggaagaga tcgaagtagt cgccgccgac ctggcgggtg ggcagcgtgg
     1201 cgcccgcgag ctccaggccg gggatccgag gaagctcccg cggcaggatc tcccgctgga
     1261 tcgcggcagc cagctcgagc tcccgctcga tgcgctcttt ctcgagcgcc gcgcggtgaa
     1321 ggcgagtcgt ctcgatcgcg gccgccgcct gattcgcgaa gagggaaaga aggcgcgcgt
     1381 cgccgggcgt gaagtcgagc acgcgtccgt cgcgcgactc cttgtccgcg actgcgagca
     1441 cgccgaggcg ccgccctgga actgcgatgg agaccgcgag acacttctcg catccgttca
     1501 gacgcgc
//
LOCUS       DASWAV010000463         1623 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U2665
            k141_344610, whole genome shotgun sequence.
ACCESSION   DASWAV010000463 DASWAV010000000
VERSION     DASWAV010000463.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960316
            Sequence Read Archive: SRR10260007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 1623)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1623)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:47:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,929
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1623
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_344610"
                     /isolate="SMAG_U2665"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.36444 N 132.31889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..316
                     /locus_tag="VF958_09640"
     CDS             <1..316
                     /locus_tag="VF958_09640"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003386863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX9149410.1"
                     /translation="SGARIVVVAPQGEDLGYVPLEAFLSGKPVLTTDDAGGPLEFVSD
                     GETGFVVPPRPEALGAALAKAWDREDALAALGRRGLERAARLSWDETIGRLMAAAGLA
                     VP"
     gene            367..519
                     /locus_tag="VF958_09645"
     CDS             367..519
                     /locus_tag="VF958_09645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9149411.1"
                     /translation="MGELGVRPEDSRWIREHAETIASLTRRARGAGLLAYLRRLARSA
                     ARKAAR"
     gene            complement(583..>1623)
                     /locus_tag="VF958_09650"
     CDS             complement(583..>1623)
                     /locus_tag="VF958_09650"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HEX9149412.1"
                     /translation="ILSDRLAARERRGHFLGESPEPAAILKHAYAAAREIVPVTRFDL
                     YRIAPDGGVEEAWTLQRAESGEFEPALHLGSPFAGRKVDARRLRDLSVTETERSFAPK
                     DLLHGGRPRRLRLPLYSGDRLVAHLDLGSAKPIDEEAKKEIRALLVPLTASLHASRNW
                     RVAVTDELSGLASRRYFEARLAEEWARRDRYETPLAVACFDLDRFKTVNDTLGHAAGD
                     LVIRRFGEIVRGVVRASDLACRFGGEEFAALFTNTSASAARTVAERIRRMLERARFEI
                     DGRDFWVTVSAGVADAAAVSGLDPHQLLVRADQALYAAKAGGRNRTRIWTDRLTREKV
                     VAERPGPPQSDR"
BASE COUNT          240 a          552 c          596 g          235 t
ORIGIN      
        1 ttcgggcgcg cggatcgtcg tcgtcgcgcc ccagggcgaa gacctcggat acgtgccgct
       61 cgaggctttt ctctccggaa agccggttct gacgacggac gatgccggcg ggccgctcga
      121 gttcgtctcc gacggcgaga cgggattcgt cgtgccgccg cgccccgagg cgctcggagc
      181 ggcgctcgcg aaggcctggg accgcgagga cgcgctcgcc gcgctggggc ggcgaggcct
      241 cgagagggcg gcgcggctct cctgggacga aacgatcggc cgcctgatgg ccgcggccgg
      301 gctggcggtc ccgtgatcgc cgatacggaa tccctccgac gtctcgcgcg cgaatccggg
      361 cggatcttgg gcgagctcgg cgttcgcccg gaggactccc ggtggatccg cgagcacgcc
      421 gagacgatcg cgagcctgac gcgacgggcg cgcggcgccg gactcctcgc ctacctgcgt
      481 cgtctcgcgc gaagcgcggc gcggaaggca gcaaggtagc tggcgggcgc cgtcctcgaa
      541 gttgaaaaga agtagtcgag ctcttctgcg tactagggct gatcagcgat ccgattgggg
      601 aggccccggc cgctccgcga cgaccttctc gcgcgtcagg cggtcggtcc agatccgggt
      661 gcggttcctc cctccggcct tcgccgcgta gagcgcctgg tccgcccgga cgagaagctg
      721 atgcggatcg aggccggaca ccgccgcggc gtccgccacg ccggcggaca cggtcaccca
      781 gaagtcccgg ccgtcgatct cgaaccgtgc gcgctcgagc atcctgcgga tgcgctcggc
      841 gaccgtgcgt gcggcggacg cggaggtgtt cgtgaaaagc gccgcgaact cctcgccacc
      901 gaaccggcac gcgaggtcgc tcgcgcgcac gacccctctg acgatctctc cgaatcggcg
      961 gatgacgagg tctccagcgg cgtggccgag cgtgtcattg accgtcttga accggtcgag
     1021 gtcgaaacag gccacggcga gaggggtttc gtagcgatcc cgccgggccc attcctcggc
     1081 gagccgggcc tcgaaatacc gccgggaggc gagaccggac agctcgtcgg taacggccac
     1141 ccgccagttg cgtgaggcgt gcagcgaagc cgtcagcgga acgagcaggg cccggatctc
     1201 cttttttgcc tcttcgtcga tcggcttcgc gctgccgaga tcgaggtgcg ccacgagacg
     1261 gtcgcccgag tagagcggaa ggcgcagccg ccggggacga cctccgtgga gcagatcctt
     1321 tggagcgaac gatcgttcgg tctccgtcac ggagaggtcg cggagtcggc gcgcatccac
     1381 cttgcggccc gcgaacgggc tgccgagatg gagcgcgggc tcgaactctc cgctctccgc
     1441 gcgctgcagc gtccaggcct cctcgacccc tccgtccgga gcgatccggt agaggtcgaa
     1501 gcgcgtgacc ggcacgatct cgcgagcagc cgcataggcg tgcttgagga tcgccgccgg
     1561 ctcgggagac tcgccgagga agtggccgcg gcgctcgcgg gcggcgaggc gatcggagag
     1621 gat
//
LOCUS       DASWAV010000464         2287 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U2665
            k141_344643, whole genome shotgun sequence.
ACCESSION   DASWAV010000464 DASWAV010000000
VERSION     DASWAV010000464.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960316
            Sequence Read Archive: SRR10260007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2287)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2287)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:47:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,929
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2287
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_344643"
                     /isolate="SMAG_U2665"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.36444 N 132.31889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..649)
                     /locus_tag="VF958_09655"
     CDS             complement(<1..649)
                     /locus_tag="VF958_09655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011737176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2238 domain-containing protein"
                     /protein_id="HEX9149413.1"
                     /translation="MFSGLSQRGPAATSGRIRREPLALAAVGLALLAVSGIHPKDRFT
                     WVLEVAPIFVAIPILIATYRRFPLTPLAYRLIFLHALVLMAGGHYTYAEVPLGFWFRD
                     LLGLARNHYDRLGHFAQGFVPAILAREVLRRKLGLRPGGWLFFLVTCVCLAISATYEL
                     VEWWTAVASGSAAMAFLGTQGDVWDTQWDMFLALVGAIASLVLLSRVHERQLARLA"
     gene            716..1099
                     /locus_tag="VF958_09660"
     CDS             716..1099
                     /locus_tag="VF958_09660"
                     /inference="COORDINATES: protein motif:HMM:NF016836.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BON domain-containing protein"
                     /protein_id="HEX9149414.1"
                     /translation="MSKTGQRRAKVLCGAAIAIAVLGAAGCGSSEYARRTDADRTLSA
                     QVGERLAADPALSAANVAARSHSGVVALVGEAPNDEARLEAERVAGAVPGVARVDNLI
                     LVAKGDSRAAGSAPPTGALFMSRTQ"
     gene            1119..1442
                     /locus_tag="VF958_09665"
     CDS             1119..1442
                     /locus_tag="VF958_09665"
                     /inference="COORDINATES: protein motif:HMM:NF016836.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BON domain-containing protein"
                     /protein_id="HEX9149415.1"
                     /translation="MRKRIPGFLALAAAVLLVGFVAGCTTTQSPGTQIDDAGISAAVK
                     AKLTEARFSNILNVETNVTNGIVTLAGQVPNAQVKADAEAAVRSVKGVKGINNNLQVK
                     SPPTP"
     gene            1578..>2287
                     /gene="truA"
                     /locus_tag="VF958_09670"
     CDS             1578..>2287
                     /gene="truA"
                     /locus_tag="VF958_09670"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013764886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HEX9149416.1"
                     /translation="MPNWKLTLEYDGTRYRGWQAQKNTDRTVQGVLLRAAHELLGADA
                     TAGGAGRTDAGVHALAQVAHLKARRALSPGEILRGLNDALPHDVNVLSVEPAPSTFHA
                     RHDAASRTYLYRIARRRTAFDKRYVWWVRDRLDFPAMSGAASLFRGRHDFASFCENPE
                     GQSTIVVVESSEVLDRGHEIHYRVEASHFLWKMVRRLVGTLVEVGRGHLTAAAVERLL
                     SERSGEPAAWTAPPSGLFL"
BASE COUNT          414 a          757 c          788 g          328 t
ORIGIN      
        1 tcgcgagccg cgcgagctgc cgttcgtgga cccgcgagag gagaaccagg gacgcgatcg
       61 cgccgacgag cgccaggaac atgtcccact gggtgtccca gacgtcgccc tgagtcccga
      121 gaaaagccat cgccgcggaa cccgacgcca ccgccgtcca ccactcgacg agttcgtagg
      181 tcgcggagat cgccagacag acgcacgtca cgagaaagaa cagccagcct cccggacgaa
      241 ggccgagctt tcgccgcagg acctcgcgcg cgaggatcgc cgggacgaag ccctgggcaa
      301 aatgcccgag ccggtcgtaa tgattccgcg cgaggccgag gagatcccga aaccagaacc
      361 cgaggggaac ttcggcatag gtgtagtggc cgcccgccat cagaacgagc gcgtggagga
      421 agatcagccg gtaggccagc ggggtcagag gaaaccgccg gtaagtcgcg atgaggatcg
      481 gaatcgcgac gaagatcggg gcgacttcga gcacccacgt gaaacggtcc ttcggatgga
      541 tgccggacac ggcgagcagc gccagcccga cggcggcgag cgcgagcggc tctcgccgga
      601 tccgaccgga ggtggcggca ggtccccgct ggctcaggcc cgaaaacata ccggaaatgg
      661 agccatccac gcggttcgat ccgctggcac gcgccgtgct gttgttcctg tcgtgatgtc
      721 gaagacgggt caacggcggg cgaaagtgct gtgcggcgcc gcgatcgcga tcgcggttct
      781 gggagccgcg ggttgcggct cttccgagta cgcgcgccgc acggacgccg accggactct
      841 ttccgcgcag gtcggcgaga ggctggcagc cgatccagct ctctccgcgg cgaacgtggc
      901 ggcccggtct cattcgggcg tcgtcgcact cgtcggagag gcgccgaacg acgaggcgcg
      961 cctcgaggca gaacgcgttg ccggggccgt tccaggcgtc gcgcgcgtag acaacctgat
     1021 tctggtagca aagggcgact cgagggccgc gggatcagcc ccgccgacag gcgccctgtt
     1081 tatgtcccgg acccaatagg ttcggaggag gacgcatcat gaggaaacgg atccccggat
     1141 ttcttgccct ggctgcagcg gtcctgctcg tcggatttgt cgccgggtgc acgacgacac
     1201 agtcccccgg cacgcagatc gacgatgcgg gcatctcggc cgcggtcaag gcgaagctca
     1261 ctgaggcgcg gttctcgaac atcctgaacg tcgagaccaa cgtgaccaac ggaatcgtca
     1321 ccctcgcggg ccaggtgccc aatgcccagg tgaaagcgga cgccgaagcc gcggtgcgca
     1381 gcgtcaaggg cgtgaagggc atcaacaaca acctgcaggt caagagcccg ccgacgccat
     1441 agctcgaatc ggatctcgaa gcgacagagc ggttggggcc atcggcctcc aaccctctgt
     1501 cgtttctgta ttgggagcat gagccgagag gcgaacaccc tgacgcgctc ggccccggtg
     1561 ttggatagat tggagcggtg cccaactgga agctcactct cgaatacgac ggcacgcgat
     1621 accggggctg gcaggcccag aagaacaccg atcgcaccgt ccagggggtc ctgctgcgcg
     1681 cggcgcacga actcctcggt gccgacgcga cggccggcgg cgccgggcgg accgacgcgg
     1741 gggtgcacgc gctcgctcag gtcgcgcacc tgaaggcgcg gcgcgcgctt tcccccggcg
     1801 agatcctccg cggattgaac gacgccctcc ctcacgacgt gaacgtcctg tcggtcgagc
     1861 ccgccccttc gacgttccac gcgcggcacg acgcggcgag ccggacctac ctctatcgca
     1921 tcgcgcggcg gcggaccgct ttcgacaagc gctacgtctg gtgggtgcgc gatcgactcg
     1981 acttcccggc catgtcggga gcggcgtcgc tctttcgcgg gaggcacgac ttcgcttcct
     2041 tctgcgagaa cccggaaggg cagtcaacga tcgtcgtcgt cgaatcgagc gaggtcctcg
     2101 accgcgggca tgagatccac taccgggtgg aggcctcgca ctttctctgg aagatggtga
     2161 gacggctcgt cggaacgctc gtggaggtcg ggcgcggtca cctcacggcg gccgccgtgg
     2221 agcggctcct ctccgaacgg tcgggagagc cggcggcctg gaccgccccc ccttcgggac
     2281 tctttct
//
LOCUS       DASWAV010000465         5283 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U2665
            k141_582408, whole genome shotgun sequence.
ACCESSION   DASWAV010000465 DASWAV010000000
VERSION     DASWAV010000465.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960316
            Sequence Read Archive: SRR10260007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 5283)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5283)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:47:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,929
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5283
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_582408"
                     /isolate="SMAG_U2665"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.36444 N 132.31889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1083
                     /locus_tag="VF958_09675"
     CDS             <1..1083
                     /locus_tag="VF958_09675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9149417.1"
                     /translation="LFQGAANEPVDAGIKGQYQDELTVGAEMALAPTFAVGVKGMYRK
                     LGRAIEDRCDLDYTATINNESSCAMMNPGESGFWGTGGAFPWCDNAGDAFVSPTWGTC
                     SARDGVDLGPAPKIPKASRKFQGIELTARKTFTQNLWAQASYVYSKLKGNYDGAVRVA
                     SGQTDPGINADYDYPLFSRNANGRLYLDRPHQFRLDAVYTAPFGLNVGLGAFYRSGPP
                     VSRYGWYNVFYPDLLHLVTRGNDTAVSGGRLVGQYEANLSLGWTFNLGAVSITPQLYV
                     FNLLNRQGVTDVVESFNPDGTFCLDTAGCGTQINPDTGAPWLTQRNFERLGTVAYGQP
                     IPQEDWAKPSARQDPRQIRAALKISF"
     gene            1390..2274
                     /locus_tag="VF958_09680"
     CDS             1390..2274
                     /locus_tag="VF958_09680"
                     /inference="COORDINATES: protein motif:HMM:NF040439.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GWxTD domain-containing protein"
                     /protein_id="HEX9149418.1"
                     /translation="MLAFYRGASLASLDRKDEARQQFEAFLAFQPNAALDPAIYSKKI
                     ITALEDARRTLASNKDLPAQTGSLATAYRAFPKPRHEEQVSLGEDWADGPVRFLMTAD
                     ERHNFSRFSDPVSRSEYVTAFWKKHDPKPETPENEFRDEFEKRVAFADSRFTQGETAG
                     SLTDRGMVFILLGPPTYAGQKPLTSGEDTSDKAALFTYRPADVKIASMPGGTTADTVR
                     KVDKVTGPGTSMNEAAKNWREVWHYRREDLPKGIPYHQVDFQFVTKQGYGQNVLQRET
                     QVLDTLEKAKSQLRQAAL"
     gene            2340..3416
                     /locus_tag="VF958_09685"
     CDS             2340..3416
                     /locus_tag="VF958_09685"
                     /inference="COORDINATES: protein motif:HMM:NF024853.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HEX9149419.1"
                     /translation="MRKILVFAALLTQLLASPARADEETRQRLIHFFGGWYSWYPNTM
                     IQVKASHEVEIAGFETYRVNRFCESKLHQESNVALVDHAKDEVFVGEVFHDLARRMAK
                     RPFDAAADVPPIEGSLSEAYGLPVKVKLDDGARGPLKPITIVIRHAENAPVQIPGFVS
                     DDGATLMIGEFHPLSSDAQAVRRKLLDETPGIRPVKGTFYVAEFLDFQCERCRVRAPE
                     VKKTVAENGGAVESRLLPLTKVHNWAFAAAEYAAALANVDPALYPKYEESLFAREGMT
                     AAAARQIASDIAEAAGAKEKFEGELASGRARERVVADIRLAIRLGLSGTPSFLHEGNF
                     ISGERELFEAYLRGKLSPQAKSAG"
     gene            3437..>5283
                     /locus_tag="VF958_09690"
     CDS             3437..>5283
                     /locus_tag="VF958_09690"
                     /inference="COORDINATES: protein motif:HMM:NF013799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HEX9149420.1"
                     /translation="MTLRRARWISALALLLVAFGPACRKDAGQPAPGAAMTPALATPV
                     EDADVATLPARPAGENPRVIWLALDGLDWDLLDRLSHEGKMPNWARLASEGYTANLAS
                     FFPVLSPVIWTTVATGVGPDVHRVLDFQETDPVTGAKLPISGLSRAVPAVWNLASAAG
                     RKVGVVGWWATHPAEEVNGFFVSDRTSPLLFGDLPRAGAAFPASLASGVEQFVARDGA
                     VSDDELTRFIDVPASEIASARVSGAGMENPIVGLSRIVGATRVNHHIARELYDRYRPD
                     LTTVYLEGTDEIGHVFAPYSPPRLECTTEADYAKYHRAVEVYYGLIDRMLGQWMRRAK
                     EDGATLMVNSDHGFKWGEDRTCARSSQNWATAAYWHRLNGVFAAWGARVHPSKTRAKL
                     SVFDVAPTVLAVLDLPMDRRMTGKAATAAFDGVRVPARKDLFATVAVRRVSSESISAE
                     QASEYSKKLRALGYLTGGDTQPLAPAGGNRPGMTEGAYNNLGVYLRETVGDLKAAEPV
                     LLKALELRPGYGSPIFNLAVLYRMKGDDAKAIEWLFRSLDSGHSEPERTLTDWIVEYE
                     EKGKTRPARVLLEHAVQRFPENEAFARELALQKFKTSRDCPGAFATLSS"
BASE COUNT          906 a         1787 c         1692 g          898 t
ORIGIN      
        1 ctcttccagg gcgcggcgaa cgagccggtg gatgccggaa tcaagggcca gtatcaggac
       61 gagctcacgg tcggcgccga gatggcgctg gctccgacct tcgcggtcgg cgtgaagggg
      121 atgtatcgca agctcgggcg cgcgatcgaa gaccgttgcg acctcgatta cacagcaacg
      181 atcaacaacg agtcctcctg cgcgatgatg aacccgggcg agtccggctt ctggggcacc
      241 ggaggtgctt ttccctggtg cgacaatgcc ggcgacgcat tcgtctcccc gacctgggga
      301 acgtgctctg cgcgcgacgg cgtcgatctc ggccccgcgc cgaagatccc gaaggcgagc
      361 cgtaagttcc aggggatcga gctcaccgcc cgcaagacct ttactcaaaa cctgtgggcg
      421 caggcttcct acgtctactc gaagctgaag ggtaactacg acggcgcggt gcgcgtggcc
      481 tcgggtcaga ccgaccccgg aatcaacgcc gattacgact acccgctctt ctcgagaaac
      541 gcgaacgggc gtctctacct cgatcggccg caccagttcc gtctggacgc ggtctacacg
      601 gccccgttcg gtctgaatgt cggactcggt gccttctatc ggagcggtcc gcccgtttcg
      661 cggtacggct ggtacaacgt gttctatccg gaccttctgc acctcgtcac ccgcggcaac
      721 gacacggcgg tctcgggcgg acggctggtc ggccagtacg aagcgaacct ctcgctcggc
      781 tggacgttca acctgggcgc ggtttcgatc acgccgcagc tctacgtgtt caacctcttg
      841 aaccggcagg gcgtgaccga cgtcgtcgaa tccttcaatc ccgacggaac gttctgtctc
      901 gacacggctg gatgcgggac gcagatcaac cccgacacgg gagcgccgtg gctgacccag
      961 cgcaacttcg agcggctcgg caccgtcgct tacggacagc cgattccgca ggaggactgg
     1021 gcaaagccgt cggctcgtca ggacccgcgt cagatccgcg cggctctgaa gatctcgttc
     1081 tagtctcctt ctcatatttc gtcgcagggg ggccttcggg ccccccttcc ttttcggatg
     1141 gacttcagcc acccccggac gggcgtagga ttcgttattc cggaggtgtc ccatgaagcg
     1201 cgtactgctt tccttgttcg caatcctgtt tgcggtctcg cttcccgccg ccgatcttcc
     1261 gaagctcctc cagaaagcca aagagcagtt ccgcctggcg gcctactccg acgcactcgt
     1321 tactctcgac gagctcgaag cggggagcca ggggaaggtc acgagaacga tcgcgccgcg
     1381 ctcgctccgg tgctggcgtt ctatcgcggg gcgtctctgg cgtccctcga tcgaaaggac
     1441 gaggcgcgcc agcagttcga agctttcctg gcgtttcagc cgaacgccgc gctcgatccc
     1501 gcgatctact cgaagaagat catcacggca ctcgaagacg cccgtcggac cctcgcatcg
     1561 aacaaagatc tgccggcgca gaccggatcg ctcgcgacgg cgtatcgcgc attcccgaag
     1621 ccccggcacg aagagcaggt gtcgctcggc gaagactggg cggacgggcc ggtccgattc
     1681 ctgatgaccg cggacgagcg ccacaacttt tcgcgctttt ccgaccccgt ctcgcgctcc
     1741 gagtacgtca cggcgttctg gaagaagcac gatcccaaac ccgagactcc ggagaacgag
     1801 ttccgcgacg agttcgagaa gagagtggcg ttcgccgact cccggttcac ccagggggaa
     1861 acggcgggca gcctgaccga ccgcgggatg gtcttcattc ttctcggccc gcccacctat
     1921 gccggccaga agccgctcac ctccggagaa gacacgagcg acaaggcggc gctcttcacc
     1981 taccgcccgg cggacgtgaa gatcgcgtcg atgcccgggg ggacaacggc cgacacggtg
     2041 cgaaaggtcg acaaggtcac cggcccggga acgagcatga acgaggcggc gaagaactgg
     2101 cgcgaggtct ggcactaccg ccgcgaagac ctgccgaagg ggattccgta ccaccaggtc
     2161 gacttccagt tcgtgaccaa gcagggttac ggacaaaacg tactgcagcg cgagacgcag
     2221 gttctcgaca cgctggagaa agccaagtcg cagttgcggc aggccgccct ctaagccggg
     2281 atctccgtac atcgccacgg cggaatccgc gccgaggccg gcgtgttaac gttcgcgccg
     2341 tgcgcaaaat cctcgttttt gcggcccttc tcacgcagct tctcgcctcg ccggcccgcg
     2401 ccgacgagga gacgcgccag cgcctcatcc acttcttcgg cggctggtac tcctggtatc
     2461 cgaatacgat gattcaggtc aaggcaagcc acgaagtcga gatcgcgggc ttcgagactt
     2521 accgcgtcaa ccgcttctgc gagtcgaagc tccaccagga atccaacgtc gcgctcgtgg
     2581 accacgcgaa ggacgaggtc ttcgtcggag aggtcttcca cgacctcgcg cggcgcatgg
     2641 caaagcgtcc gttcgacgcc gccgcggacg tgcctccgat cgagggctcg ctctcggaag
     2701 cgtacgggct gccggtgaaa gtcaagctcg atgacggcgc tcgcgggcct ttgaagccga
     2761 tcacgatcgt catccgtcac gccgagaacg ctccggttca gatcccgggg ttcgtctccg
     2821 acgacggtgc gactctgatg atcggagagt ttcacccgct ctcttcagac gcgcaggccg
     2881 tccgccggaa gcttctcgac gagacgcccg ggatccggcc cgtcaaggga actttctacg
     2941 tcgccgaatt cctcgatttt cagtgcgagc gctgccgtgt acgcgctccg gaggtcaaga
     3001 aaaccgtagc ggagaacggc ggcgcggtcg aatctcggct cctcccgctc accaaagtac
     3061 acaactgggc gttcgccgcc gccgagtacg ccgctgcgct cgcgaacgtc gacccggcgt
     3121 tgtatccgaa gtacgaggaa tcgctcttcg cgcgcgaagg gatgacagcc gcggcggcgc
     3181 ggcagatcgc ctccgatatc gccgaggccg cgggcgcgaa ggagaagttc gaaggagagc
     3241 tcgcgagcgg gcgcgcgcgg gagcgcgtcg tcgccgacat ccgcctggcg atccgcctgg
     3301 gactttcggg gacgccgtcc ttcctgcacg agggcaactt catttccggc gagcgggagc
     3361 tcttcgaggc ctacctccgc ggcaagctgt cgcctcaggc gaagtcggcc ggctagcatc
     3421 cttcgtgtcg agccgaatga ctctgcgccg ggcccgctgg atctccgctc tcgccctgct
     3481 cctcgtggcc ttcggcccgg cgtgccgaaa agacgccggc cagcccgctc cgggcgccgc
     3541 gatgactccc gccctggcga cccccgtgga agacgccgac gtcgcgacgc ttccggcgcg
     3601 cccggccggg gaaaaccctc gcgtcatctg gctggcactg gacggcctcg actgggacct
     3661 tctcgaccga ctgtcgcacg aaggcaagat gcccaactgg gcgcgcctcg cgtcggaggg
     3721 atacaccgca aacctcgcga gcttctttcc cgtcctttcg ccggtgatct ggacaaccgt
     3781 cgcgaccggc gtcggccccg acgtccaccg ggtgctcgac ttccaggaga ccgaccccgt
     3841 caccggcgcg aagctcccga tctccggcct gtcgcgcgcc gtccccgcgg tgtggaatct
     3901 cgcgtccgcc gcagggcgga aggtcggcgt cgtcggatgg tgggcgacgc atcctgccga
     3961 agaggtcaac ggtttcttcg tgtcggaccg cacgagcccc ctcctgttcg gcgatcttcc
     4021 gcgcgccggg gcggcgtttc cggcttcgct cgcttccggc gttgagcagt tcgtcgctcg
     4081 cgacggcgcg gtgtccgacg acgagctcac gcggttcatc gacgtgcccg cctcggaaat
     4141 cgcgtcggcc cgtgtgagtg gggccggtat ggagaatccg atcgtcgggc tgtcgcgcat
     4201 cgtgggcgcg acgcgcgtca atcaccacat cgcccgcgag ctgtacgacc gctaccgtcc
     4261 cgacctgacg acggtgtatc tcgagggcac cgacgagatc ggccacgtct tcgcgcccta
     4321 ctcgccgccg cgcctcgaat gcacgaccga ggccgactac gcgaagtacc accgtgccgt
     4381 cgaggtctat tacggtctca tcgaccggat gctgggccag tggatgaggc gcgcgaagga
     4441 ggacggcgcc accctgatgg tcaactcgga ccacggcttc aagtggggag aggaccggac
     4501 ttgcgcgcgg tcctcccaga actgggcgac ggcggcctac tggcatcggt tgaacggcgt
     4561 gtttgccgca tggggcgctc gcgtgcatcc gtcgaaaacg cgcgcgaagc tctcggtctt
     4621 cgacgtggcg ccgacggtgc tcgccgtgct ggatcttccg atggaccgcc ggatgaccgg
     4681 gaaggcggcg accgcggcct tcgacggtgt ccgggtccct gcccgaaagg acctcttcgc
     4741 aaccgtcgcc gttcgccgcg tgtcttcgga gtcgatttcc gccgagcagg cgagcgagta
     4801 ttcgaagaag ctccgcgcgc tcggatatct gacgggaggc gatactcagc cactcgcccc
     4861 cgcgggcggg aaccgcccgg gcatgaccga aggcgcgtac aacaacctcg gcgtctacct
     4921 tcgggagacg gtcggcgacc tgaaggcggc cgagcccgtt cttttgaagg cgctcgagct
     4981 tcgtccaggc tacggctcac cgatcttcaa cctcgccgtg ctctaccgga tgaaggggga
     5041 cgacgcgaag gcgatcgagt ggctgttccg atccctcgac tccggccatt cggaaccgga
     5101 gcggaccttg accgattgga tcgtcgagta cgaggagaag gggaaaaccc gtcccgcgcg
     5161 agtgctcctg gagcacgccg tgcagcggtt ccccgagaac gaggcattcg cgcgcgaact
     5221 ggcgctccag aagttcaaga cctcgagaga ctgcccggga gcgtttgcga cgctctcctc
     5281 ttt
//
LOCUS       DASWAV010000466         6084 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U2665
            k141_383651, whole genome shotgun sequence.
ACCESSION   DASWAV010000466 DASWAV010000000
VERSION     DASWAV010000466.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960316
            Sequence Read Archive: SRR10260007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 6084)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6084)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:47:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,929
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6084
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_383651"
                     /isolate="SMAG_U2665"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.36444 N 132.31889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..324
                     /locus_tag="VF958_09695"
     CDS             <1..324
                     /locus_tag="VF958_09695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9149421.1"
                     /translation="KGRETREAARAGKEYMEQWLERGGYKLADKGAELTIEGNVFNAW
                     EPGTGARIWGGWMANPGVGVEVIARDSSGKVVGEVRHKNRGSTVEDAVENALEEVAKA
                     ISDGR"
     gene            672..1454
                     /locus_tag="VF958_09700"
     CDS             672..1454
                     /locus_tag="VF958_09700"
                     /inference="COORDINATES: protein motif:HMM:NF014605.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HEX9149422.1"
                     /translation="MGYPLKGSDVTTAIRVLKEDPQASIVEFFTESPFGPSLRSVVSA
                     RNTKQRLYVQTIDRHDLVFAIGPAGTGKTYLAVAMAAATLLEKKVKRIVLARPAVEAG
                     ERLGFLPGDMVEKVNPYLRPLYDALYDILGYERTAKLLERGIIEIAPIAFMRGRTLND
                     AFIILDEAQNTTSEQMKMFLTRIGFGSKAVVTGDITQVDLPQGKTSGLREAQQILSSI
                     DGIHFVLFDERDVVRHPLVQSIISAYDALDRERESATARSAR"
     gene            1638..3890
                     /locus_tag="VF958_09705"
     CDS             1638..3890
                     /locus_tag="VF958_09705"
                     /inference="COORDINATES: protein motif:HMM:NF019317.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDIG domain-containing protein"
                     /protein_id="HEX9149423.1"
                     /translation="MRPFFWGALYVLVTTISFAPTLTFRTVPATPGAIASRDVVAPRD
                     MIVPDPETTTRRKAEAAAEVLPVYDSDGAAPARFEEELRNSFARARAARERGKSRGEV
                     TQEIRDAFNLPIGDEALVALARLDFSRPLEDLLVATGLDLYRSEIVDNRELFLEQRRG
                     ILVRDTSTGREQRRRDVPRAIEYGSEAKSEVALRLADSALTERERGEVAAFVAASLRP
                     NLTFNASETAHRREEASRAVESVFTKIPRGKVIVRKGDEITPRTSMWIAAVRASLADP
                     SSWVKVAGILILQILAALAFWMDARRLTRRKRERPPETIYASIISAGVLFALLTRAFF
                     VLAQGFSSSFEGAATAAATYYALPFAAGSIVASLVAGMGPALLFAAIHAVGAGVLMGQ
                     SFPFTLFALVGSLAGIFGMGRLRSRSVLLGMGAMVAAANVVSIAAIQLLNAEPIRWTF
                     ALDALGGLVGGLLVSATVGLVLPIFEHFFHVTTEIRLLELSNQNLPLLRNLALEAPGT
                     YQHALMVGHLAEAAAEAIGSDALLARVSGYYHDIGKTKMPAYFIENQTKGLNRHDRLE
                     PSMSALIIAAHVKEGLELAKKARLPEPIVAAIREHHGTKLIRYFYQKALTKSEPGGSA
                     INETEYRHPGPKPGTRITGILMISDAVEAASRTLVEPTRQKIRAMIQTIVDDCLRDGQ
                     FDECDLTMRDLALIVDALENTVTTIFHHRIDYPGFDFEPERTKRKTGDVPSAPSAAAA
                     ADRRTGPRPA"
     gene            3904..4335
                     /gene="ybeY"
                     /locus_tag="VF958_09710"
     CDS             3904..4335
                     /gene="ybeY"
                     /locus_tag="VF958_09710"
                     /inference="COORDINATES: protein motif:HMM:TIGR00043.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HEX9149424.1"
                     /translation="MLEVAVRSSAAGAPSTNRVRRLMRRAARAVGGRHREISVYFCGD
                     RRMATLNGRWRGKNVSTDVLAFPAGGAEGGFLGDVVISVPFAARQAHRCGEPTSREID
                     RLLVHGYLHLLGYDHETDDGEMNALEARLRKKFGIAEGGAS"
     gene            4332..5564
                     /locus_tag="VF958_09715"
     CDS             4332..5564
                     /locus_tag="VF958_09715"
                     /inference="COORDINATES: protein motif:HMM:NF013741.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HEX9149425.1"
                     /translation="MIPSVASVFCYLAFLLFESFALALDRLSPIKTRGLLEEHPERAR
                     ILSGPGEVEIIRTTTKVLVQVLLLAGLLTVVSGFQTVGIEPAWAWGGGVFLLGWLLSE
                     VALLKFVATADPERIVSRLLPVVTAASWLLLPLAIPIRKILAERAPAETPPATEEEVQ
                     AYIDVGREEGILEKEEEKLLLSIVDFGDTRVREVMTPRTDIAYMDVASSLTVLADLFV
                     ETKYSRIPVVRGSIDRVVGIVHVKDLLESIRKGSSGRIADLVREAYFVPETKKVSELL
                     REFQRRHLYMAIVVDEYGGVSGLVTVEDLLEEIVGEISDEHEDEHEMVTPAGEQRYSV
                     SGKANIALVRELFDRGPEEEEYSTLGGFLAARLGHIPRPGETHSEADLLFTVEEADRR
                     RVHRVRIEPVAVPVQREP"
     gene            5561..>6084
                     /gene="era"
                     /locus_tag="VF958_09720"
     CDS             5561..>6084
                     /gene="era"
                     /locus_tag="VF958_09720"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HEX9149426.1"
                     /translation="MTDQNSRVPDSPIPISGQPVFRAGEVAVVGRPNAGKSTLVNRLV
                     GEKVAIVSDKPQTTRKRILGIARRPGAEIALVDTPGIHRPDHRMNAVMLRDATDALST
                     ADLVLLVVDAAEGGKEGRVLDLLSRAGRPAFLALNKIDLVAKEGLLPMIAEFAAPGLF
                     REIVPISAKTGDGV"
BASE COUNT         1001 a         2034 c         2056 g          993 t
ORIGIN      
        1 aagggacgcg agacgcgcga ggcggcccgt gcgggcaagg agtacatgga acagtggctc
       61 gagcggggcg gctacaagct cgccgacaag ggagcggagc tgacgatcga gggcaacgtg
      121 ttcaatgcgt gggagcccgg caccggcgcg cggatctggg gcggctggat ggccaacccg
      181 ggagtcggag tcgaggtcat cgcccgcgat tccagcggaa aggtggtcgg cgaggtccgg
      241 cacaagaacc gaggttcgac cgtcgaagac gccgtcgaga acgccctcga ggaggtcgcc
      301 aaggcgatct cggacggccg ctgacagccg agcgacgaaa aacgccgcgc cgatctaagc
      361 cttctggaca ataggtttgc cgtcgagagg cgggtccccg gcaacggggc ccgccttttg
      421 ctgcctattg ggcgtggtag cgtaatttcc ggaccggagg agtaaccaag attcccgacc
      481 cgaacccgac cgtcacgctt cccgaggcgg gatttactgc ccttttcggc atcgcggacg
      541 agaacctgag ggccatcgag gaggcgttcc gagtccgcct cgccgcccgc ggcaatgagg
      601 tctcggtcgt cggcggagag ggagaggaga gcgcacgaaa gctcctttcg gctttctccg
      661 agctcgccac gatgggctat ccgctgaagg gaagcgacgt gacgacggcg atccgcgtgc
      721 tgaaagagga cccgcaggcc tcgatcgtcg agttcttcac cgagagcccc ttcgggccgt
      781 ccctgcggtc ggtcgtctcg gcgcgcaata cgaagcagcg gctctacgtc cagacgatcg
      841 accggcacga cctcgtcttc gcgatcgggc cggcagggac gggcaagacc tacctcgccg
      901 tcgccatggc cgcggcgacc ctcctcgaga agaaggtcaa gcggatcgtt ctcgcgcggc
      961 ccgcggtcga agccggcgag cggctgggct ttctccccgg cgacatggtc gagaaggtca
     1021 acccgtacct gcgtcccctc tacgatgccc tgtacgacat cctgggatac gagaggaccg
     1081 cgaagctcct ggagcgcggc atcatcgaga tcgcgcccat tgccttcatg cgcgggcgga
     1141 cgctgaacga cgcgttcatc atcctcgacg aggcgcagaa cacgacgtcc gagcagatga
     1201 agatgttcct gacccggatc gggtttggat cgaaggccgt cgtcaccggg gacatcaccc
     1261 aggtcgacct gccgcagggc aagacctcgg gcctccgcga ggcccagcag atcctctctt
     1321 cgatcgacgg gatccacttc gtgctcttcg acgagcgcga cgtggtacgc catccgctcg
     1381 ttcagtcgat catctcggcc tacgacgctc tggaccggga gcgcgagtcg gcgaccgcgc
     1441 gctcggcccg ctaagaaaat gaggggccag gtcttcacgc gtcgccgtga gcggggtcag
     1501 gtctccacgt gccacttcgc gcgccgaagt aactgatggc aagcgatacg gctccacgga
     1561 ccaagcccgc caccttccgg cggcgccggc agacggcccg tgagacgggg gccgcggggc
     1621 cgatccgacg cgcaggtatg cgtcccttct tctggggggc gctctacgtt ctcgtgacca
     1681 cgatctcgtt cgcgccgacg ctgacgttcc gcaccgtgcc cgccactccg ggagcgatcg
     1741 cgtcgcgcga cgtcgtggcg ccacgcgaca tgatcgttcc cgatccggag acgacgacgc
     1801 ggcggaaggc ggaggccgcg gcggaggtcc ttccggtgta cgactcggac ggggcggcgc
     1861 ccgcgcggtt cgaggaggag ctgcggaact cgttcgcgcg cgcccgcgcc gcacgggaac
     1921 gcgggaaatc gcggggagag gtcacgcagg agattcgcga cgccttcaac cttccgatcg
     1981 gggacgaggc gctcgtggcg ctcgcgcggc tcgacttctc gcgtccgctg gaagacctgc
     2041 tcgtcgcaac cggcctcgat ctttaccgca gcgagatcgt cgacaaccgg gagcttttcc
     2101 tcgagcagcg acggggcatc ctcgtgcgcg acacatcgac cggtcgggag cagagacggc
     2161 gcgacgtccc ccgcgcgatc gagtacgggt ccgaagcgaa atccgaggtc gctctccggc
     2221 tcgccgacag cgccctcacg gagcgcgagc gcggagaggt cgcggcgttt gttgccgcgt
     2281 cgcttcgccc gaacctgacc ttcaacgcgt cggaaaccgc ccaccggagg gaagaggctt
     2341 cccgcgcggt cgagagcgtc ttcacgaaga tcccccgcgg aaaagtgatc gttcgcaagg
     2401 gcgacgagat cacgccgcgc acttcgatgt ggatcgcggc ggtgcgtgcc tcgctcgccg
     2461 atccgtcttc atgggtgaag gtcgcgggga tcctgatcct gcagatcctt gccgctctcg
     2521 cgttctggat ggacgcccgg cgcctcactc gccgcaagcg cgagcggccc cccgaaacga
     2581 tctacgcctc gatcatttcg gcgggagtcc tgttcgcgct cctgacccgc gccttcttcg
     2641 tgctcgcgca gggcttctcc tcgagcttcg agggcgcggc gacggcggcc gcgacgtact
     2701 acgcgcttcc gttcgccgcg ggctcgatcg tcgcgagcct cgtcgccggc atgggccccg
     2761 cgctcctctt cgccgcgatc cacgcggtcg gcgccggcgt tctcatgggc cagagcttcc
     2821 cgttcaccct gttcgccctc gtcgggtcgc tggccgggat cttcggcatg ggccgcctgc
     2881 gctcgcgcag cgtgctgctc gggatgggag cgatggtcgc ggcggccaat gtcgtctcga
     2941 tcgccgccat tcagctcctg aacgccgagc cgatccggtg gaccttcgct ctcgacgctc
     3001 ttggaggtct cgtcggcggg ctgctcgtct cggcgacggt cggtctcgtc ctacccatct
     3061 tcgaacactt cttccacgtg acgaccgaga tccgccttct cgagctgtcc aaccagaacc
     3121 tgcccctgct gcggaacctc gcgctcgaag cgccgggcac gtatcagcac gcgctcatgg
     3181 tgggccacct cgccgaggcg gcggccgaag cgatcgggtc cgacgcgctc ctcgcgcggg
     3241 tgtcggggta ctaccacgac atcggcaaga cgaagatgcc cgcgtacttc atcgagaacc
     3301 agacgaaggg gttgaaccgc cacgaccggc tcgagccgtc catgagtgcc ctcatcatcg
     3361 cggcgcacgt caaggagggc ctggagcttg cgaaaaaagc ccggttgccc gagcccatcg
     3421 tcgccgcgat ccgggagcat cacggaacga agttgatccg ctacttctac cagaaggcgc
     3481 tgacgaagtc ggagccgggc gggagcgcca tcaacgagac cgagtaccgg catcccggac
     3541 caaaaccggg aacgcggatc accgggatcc tcatgatctc cgatgcggtg gaggcggcgt
     3601 cgcgcaccct cgtcgagccg acgcgccaga aaatccgggc gatgatccag acgatcgtcg
     3661 acgactgcct gcgcgacggc cagttcgacg agtgcgacct cacgatgagg gacctcgccc
     3721 tgatcgtcga tgcgctcgag aacacggtga cgacgatctt ccaccaccgg atcgattacc
     3781 cgggcttcga cttcgagccc gagcgcacga agaggaagac cggcgacgtc ccgtcggcgc
     3841 cttccgcggc cgccgcagcg gatcgtcgaa cgggtccgcg accggcctga ggtgtcgaat
     3901 gagatgttgg aagtcgcggt ccggtccagc gcggcgggcg ctccgtcgac gaaccgggtc
     3961 cggcgcctca tgcggcgtgc ggcgcgggcg gtcggcggtc gccaccggga gatctcggtc
     4021 tacttctgtg gcgaccggcg catggcgacg ttgaacggcc ggtggcgggg caagaacgtt
     4081 tccaccgacg tcctcgcgtt ccccgcgggg ggcgcggaag gcgggtttct tggtgacgtc
     4141 gtgatctcgg tgcccttcgc ggcgcgccag gcacaccggt gcggcgagcc gacctcccgc
     4201 gagatcgacc ggcttctcgt acacggatac ctgcaccttc tcgggtacga ccacgagacg
     4261 gacgacgggg agatgaacgc gctcgaggcg cgtctgcgga agaagttcgg aatcgcggaa
     4321 gggggcgcaa gttgatcccc tccgtcgcct cggttttctg ctatctcgcg ttcctccttt
     4381 tcgagagttt cgcgctggcg ctcgaccgcc tctccccgat caagacccgc gggctcctcg
     4441 aagagcatcc cgaacgcgcg cggattctct cggggcccgg cgaggtcgag atcatccgca
     4501 cgaccacgaa agtcctcgtg caggtcctgc tgctcgccgg cctcctgacg gtcgtttcgg
     4561 ggttccagac cgtcgggatc gaacccgcgt gggcctgggg cggcggtgtc ttcctgctgg
     4621 ggtggctcct ctcggaagtc gcgctcttga agttcgtcgc gacggcggac cccgagcgca
     4681 tcgtctcgcg gcttctgccg gtcgtcacgg ccgcgtcctg gctgcttctt ccgctggcga
     4741 ttccgatccg caagatcctc gccgaacgtg cgccggcgga aacgcccccc gcgaccgaag
     4801 aggaagtcca ggcctacatc gacgtcgggc gtgaagaggg aatcctcgag aaggaagagg
     4861 agaagctcct tctctcaata gtggatttcg gcgatacacg cgtgcgagag gtcatgacgc
     4921 cgcgcaccga catcgcgtac atggacgtcg cctcttcgct caccgtgctc gcggatctct
     4981 tcgtcgagac caagtactcg cggatccccg tcgtgcgcgg ttcgatcgac cgcgtcgtcg
     5041 gcatcgtcca cgtcaaggac ctgctcgagt cgatccggaa ggggagctcg ggaaggatcg
     5101 ccgatctcgt ccgcgaggcg tacttcgtcc cggagacgaa gaaagtctcg gagctcctcc
     5161 gagagtttca gaggcgccac ctttacatgg cgatcgtcgt ggacgaatac ggcggagtgt
     5221 cggggctggt gaccgtcgag gatcttctcg aggagatcgt cggcgagatc tccgacgagc
     5281 acgaggacga gcacgagatg gtcactccgg cgggcgagca gaggtactcc gtctcgggca
     5341 aggcgaacat cgcgctcgtg cgggagctct tcgaccgcgg ccccgaggaa gaggagtact
     5401 cgacgctcgg cggttttctc gccgcgcggc tcggccacat tccccggccg ggggagacgc
     5461 acagcgaagc ggacctgctc ttcaccgtgg aagaggcgga ccgccgccgg gttcatcgcg
     5521 tccgcatcga gccggtcgcc gtcccggtcc agcgcgagcc ttgacggacc agaattcccg
     5581 ggttcccgat tccccaattc cgatttccgg ccagcccgtc ttccgcgcgg gcgaggtggc
     5641 ggtcgtcggg cgtccgaacg cgggcaagtc gacgctcgtg aaccggctgg tcggcgagaa
     5701 agtcgcgatc gtttccgata agccgcagac gacgaggaaa cgcattctcg ggatcgcgcg
     5761 ccgtccgggc gccgagatcg cgctcgtgga cactccggga atccaccgcc ccgatcaccg
     5821 gatgaacgcc gtcatgttga gggacgcgac ggacgcgctt tccaccgcag acctcgttct
     5881 cctggtcgtc gacgcggccg agggcggaaa ggaggggcgc gtcctcgatc tgctctcccg
     5941 ggccggtcga cccgcttttc ttgccctcaa caagatcgat ctcgtggcca aggaaggact
     6001 gctgccgatg atcgcggagt tcgccgcgcc cgggctcttc cgtgagatcg tgccgatctc
     6061 ggcgaagacg ggcgacgggg tcga
//
LOCUS       DASWAV010000467         1854 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U2665
            k141_665285, whole genome shotgun sequence.
ACCESSION   DASWAV010000467 DASWAV010000000
VERSION     DASWAV010000467.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960316
            Sequence Read Archive: SRR10260007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 1854)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1854)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:47:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,929
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1854
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_665285"
                     /isolate="SMAG_U2665"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.36444 N 132.31889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1617)
                     /locus_tag="VF958_09725"
     CDS             complement(<1..1617)
                     /locus_tag="VF958_09725"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX9149427.1"
                     /translation="MNLGRRRLLPDARAAILIAACVVCYANGLTGDFTYDDKAIVRDN
                     ARLRAPAGVVSIFTTSYFGGPRGQGTGYRPILLVTYAIQRWLHGGAVFGFHVVNVALH
                     VIVTLLLARLLAQLGVPDAVAFGAALLFAVHPIHVEAVTSLVGRGETLAAAFVLGVLL
                     LALRYRRERRARGAVLALLLLCYALGILSKESAAVAPALVLLAYWRLETGGTAERLKG
                     ALKLGLPIAGGCVVVLAASLAARRAVLGGVIKASNFRIFELENPLAHVNTGERVTNAA
                     AILFRYVGRMIVPLRLSADESAWSIPVVHGFDPVGLAALVLGAGLLIAAVAREREERD
                     VAFGVLFFAAAFVTTSNLLFSIGTILAERVAYLPSAGFALALSAVLLGSAPARAPSPR
                     RSAIFLVIVLLYAARTVVRNPVWDSDDSLFADTALTSPQSAKAHYNLAWISAERGKLP
                     LALEEYTHATRIYPKYFDAWAGKGFVEQRMGKLAEAERSLEQSIAVSPKYENGFFRLG
                     EVRELRGNLAGAEEAFAEGLAKNPKSTPLAF"
     gene            complement(1630..>1854)
                     /locus_tag="VF958_09730"
     CDS             complement(1630..>1854)
                     /locus_tag="VF958_09730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012524742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX9149428.1"
                     /translation="LLPSSVRRVVRPATAIGDYRTRVAAHRRRLVAEALDRSGGNRTR
                     AAKELGLSRQALLYLMKELGIVSSSRTAPK"
BASE COUNT          335 a          628 c          636 g          255 t
ORIGIN      
        1 gaacgcgagc ggggtcgact tcgggttttt cgcgaggcct tccgcgaaag cttcttcggc
       61 gccggcgaga tttcctcgca gctcgcgcac ttctccgagc cgaaagaacc cgttctcgta
      121 cttcggggag accgcgatcg actgctcgag cgagcgctcc gcctcggcga gctttcccat
      181 tcgctgctcg acgaacccct tccccgccca ggcgtcgaag tacttcgggt agatccgcgt
      241 cgcgtgggtg tactcctcga gagcgagagg aagctttccc ctttccgcgg aaatccatgc
      301 gaggttgtag tgcgccttcg cgctctgcgg tgaggtcaga gccgtatcgg caaagaggct
      361 gtcgtcgctg tcccataccg ggtttcggac gaccgtgcgc gccgcgtaca ggagcacgat
      421 cacgaggaag atggcgctcc gtcgtggtga cggcgcccgt gccggggcgc tcccgagcag
      481 caccgcggag agagcgagtg cgaaccctgc cgaagggagg tacgccactc gctccgcgag
      541 aatcgtcccg attgagaaga gaaggttcga ggtcgtgacg aacgcagcgg cgaaaaagag
      601 cactccaaac gcgacgtcac gctcctcgcg ctcccgcgcc accgcggcga tcaagagacc
      661 ggcgccgagg acgagcgccg ccaggccgac cggatcgaag ccgtgcacga cggggatcga
      721 ccaagcggac tcgtcggccg aaaggcgaag cggcacgatc atccgtccga cgtagcggaa
      781 gaggatggcg gccgcgttcg tcacacgctc tcccgtgttg acgtgcgcga gcgggttctc
      841 gagctcgaag atccggaaat tcgacgcctt gatcacgcct ccgagaaccg cccgcctcgc
      901 cgcgaggctg gcggcgagga cgaccacgca gccccccgcg atcgggagac cgagcttgag
      961 cgcccccttc aaccgctcgg cggttccgcc cgtctcgagc ctccagtacg cgagcagcac
     1021 gagggccggc gcgaccgcgg cgctctcctt cgaaaggatc ccgagcgcgt agcagaggag
     1081 gaggagtgcg aggacggcgc cccgcgccct gcgctcgcgc cgataccgca gcgccagcag
     1141 gagcaccccg agcacgaagg ccgccgcgag cgtttcgccc cgtccgacga ggctcgtgac
     1201 ggcttccacg tggatggggt ggaccgcgaa gaggagcgcg gctccgaagg ccaccgcatc
     1261 cgggacgccg agctgcgcca gcagccgcgc gaggagcagc gtgacgatga cgtgcaaggc
     1321 gacgttgacg acgtgaaagc cgaagacggc gccgccgtgc agccaccttt ggatcgcgta
     1381 cgtgacgaga aggatcggcc gataccccgt cccctgcccc cgcggaccgc cgaagtagga
     1441 cgtcgtgaag atcgagacga cacccgccgg cgcgcggaga cgcgcgttgt cccggacgat
     1501 tgccttgtcg tcgtaggtga agtcaccagt caggccgttc gcgtagcaga cgacgcacgc
     1561 cgcgatgaga attgccgcgc gagcgtccgg cagaagtcgt cgccgaccga ggttcatcgg
     1621 cggcgaactc tactttggag cggtccgact cgacgaaacg atcccgagct ccttcatcag
     1681 gtacagcagc gcctgccggg agaggcccag ctccttcgcc gcgcgggtgc ggttcccgcc
     1741 ggaccgatcg agcgcttcgg cgacgagccg acgccggtgg gcggcgacac gcgtgcggta
     1801 gtccccgatc gccgtcgccg gccgaacgac gcgacgaacg gacgacggaa ggag
//
LOCUS       DASWAV010000468         2451 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U2665
            k141_746636, whole genome shotgun sequence.
ACCESSION   DASWAV010000468 DASWAV010000000
VERSION     DASWAV010000468.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960316
            Sequence Read Archive: SRR10260007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2451)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2451)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:47:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,929
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2451
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_746636"
                     /isolate="SMAG_U2665"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.36444 N 132.31889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            407..2164
                     /locus_tag="VF958_09735"
     CDS             407..2164
                     /locus_tag="VF958_09735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9149429.1"
                     /translation="MALSLLGGVLAAAALLAQAQPPILAPGSAVPNWTVPAFHAESAE
                     GSLRPMTDIGFLSGAFVPMVPCRVFDTRLANGAYGGPRLVANVTRSFDIDSSACGPFP
                     TGGILAFSMNFGAILPDNANGFITIWPAGATMPTVSTINPIQGTVVANAAIVPNGTGG
                     SISVFPNTGVHLYGDINGYFTVGLNAGELFAQIANNAGGGAGTFINLANTNNTAGVYG
                     IVGPGFADNQCCAPTGVIGKGAIAGVAGSSPDRGTVGILVNSAGNLIAEGQLGRNGGG
                     GVAYGANAFTNTAVNGSAGVLGTATGGSSYIFGVKGVSASTSLDSAGVKGVSGYGDPL
                     GDTNDCGPCYAQGVRGVSDGGGLGVIGIARGGTAVVGVKLNPSDNGTLNAGYLGTPFG
                     YAVYAQGGYGGTGAKFFVEPHATDPSLVIKYIALEGPESGTYFRGKGRFQNGIATIDV
                     PEDFRMVTDRDGLSIQVTPIGEMATVAVQSIGLDRIVVRGSRNVEFFYTVNGVRRSFK
                     DHVAIQPSHGEYMPEKEAVTLESHRYLTADQQRVLVQNGTYQPDGKVNMETAHRLGWD
                     RIWAERERPTPQPSEPSSP"
BASE COUNT          482 a          762 c          774 g          433 t
ORIGIN      
        1 atccccggaa atgcaacgac agctcgtcgg acccgagggg atctcttccg cgtacaaacc
       61 ggccattcga gtcaaccgac gacgatcgct cattggatcg atcggccaga atccggcgct
      121 acgagctcgt cgccggagga accaggattc cacagttcgt gaacgacgga tgaagattcc
      181 ccgttcgcga cctcgctcaa atcctcggtt gtttcggtag aatcagcgca ctgctctcgg
      241 ggtgcgtttt caggaacgac gacctctcct cgcggaagaa gttttgaatc aggacgggcc
      301 gcggcggacc ggaaggagga gaactataga gaaagcgact tgttctcaat cggcaattct
      361 tttcgaaaca aggaggaatc gcatgaaccg gtttcctgtt acgaaattgg ctctcagcct
      421 cctcggcggc gtgcttgctg ccgccgcact tctcgcccag gcgcagcctc cgattctcgc
      481 cccgggctcg gccgtaccca actggaccgt gccggccttc cacgcggaga gcgcggaagg
      541 cagtctgcgg ccgatgaccg acatcggctt tctctcgggg gctttcgtcc ccatggttcc
      601 gtgccgggtc ttcgacacgc gtctggcgaa cggcgcatac ggcgggccgc ggttggtcgc
      661 gaacgtgacc cgcagcttcg acatcgacag cagcgcctgc gggccgtttc cgacgggagg
      721 gatcctggcc ttctcgatga acttcggcgc catcctgccg gacaacgcca atggattcat
      781 cacgatctgg ccggccgggg cgacgatgcc gaccgtctcc acgatcaacc cgatccaggg
      841 caccgtcgtc gcgaacgccg cgatcgtgcc gaacggcacg ggcgggagca tcagtgtgtt
      901 tccgaacacc ggcgtccacc tgtacggcga catcaacggc tacttcacgg tcggcctcaa
      961 cgccggcgag ctgttcgctc agatcgcgaa caatgccggc ggcggagcgg gcaccttcat
     1021 caacctcgcg aacacgaaca acaccgccgg ggtttacgga atcgtgggac ccggattcgc
     1081 ggataaccag tgctgcgctc ccaccggagt gatcgggaaa ggcgccatag caggcgtcgc
     1141 cggttcctcg ccggatcggg gcacggtcgg aatcctcgtg aattccgccg ggaacctcat
     1201 cgcggaaggg caactgggaa gaaatggggg cggcggcgtt gcgtatgggg ccaacgcctt
     1261 caccaacaca gcggtgaacg ggagcgcggg tgtgctcgga acggccaccg gagggtcgag
     1321 ctacatcttc ggcgtgaagg gtgtcagcgc gagcacttct ctcgactccg cgggagtcaa
     1381 gggggtgtcg ggctacggcg atccactcgg ggacacgaac gactgcggac cgtgctacgc
     1441 gcagggcgtg cgaggagtca gcgatggtgg cggtctcggg gtcatcggaa tagcccgggg
     1501 aggcacggcc gtggtcggag tcaagctcaa cccgtccgac aacgggaccc tgaacgccgg
     1561 ttatctcggc accccgttcg gctatgccgt gtacgctcag ggaggttacg gcggtacggg
     1621 cgccaagttc ttcgtcgagc cgcatgccac cgacccctcc ctcgtaatca agtacatcgc
     1681 gctcgagggc cccgagtccg gaacgtattt ccgtggcaag ggaaggttcc agaacggcat
     1741 cgcgacgatc gacgtcccgg aagactttcg catggtgacg gatcgagatg gcctgagcat
     1801 ccaggtgact ccgatcggcg agatggcgac ggtcgccgtg cagtcgatcg gcctcgaccg
     1861 gatcgtcgtc agaggatcgc gaaacgtcga gttcttctat acggtcaatg gcgtgcggcg
     1921 gtcgttcaag gaccacgtgg cgattcaacc gtcccatggg gaatacatgc ccgaaaagga
     1981 agcggtgacg ctggaatccc accgctacct cactgcggat caacagcgcg ttctcgttca
     2041 gaacgggact taccagccgg atggcaaggt caacatggag acggcgcacc gtctcggctg
     2101 ggatcggatc tgggcggaac gcgagcggcc aacaccccag ccgagtgagc cgtcgagtcc
     2161 gtagatctcc atcccacgac tttctcgtga agggccgcgg atctccgcgg cccttctttt
     2221 ctcgaccgcg ggcatcccgc cgtcttttct cttcagaaaa agatcaactg agagcaagcg
     2281 aggaaaatcg ctcatcgggc gcgccaacgc acatccgaac tcaccgtcgc aatcggcttg
     2341 gtgaacggca gatgaacagg tttcgtctcc tcacgaaaag agaccgacgg ccgattccgt
     2401 tgagctaaaa tcggcgcact gttcggggtg cttttcacgg acgacctgtc t
//
LOCUS       DASWAV010000469         3352 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U2665
            k141_106082, whole genome shotgun sequence.
ACCESSION   DASWAV010000469 DASWAV010000000
VERSION     DASWAV010000469.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960316
            Sequence Read Archive: SRR10260007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 3352)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3352)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:47:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,929
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3352
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_106082"
                     /isolate="SMAG_U2665"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.36444 N 132.31889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..521)
                     /locus_tag="VF958_09740"
     CDS             complement(<1..521)
                     /locus_tag="VF958_09740"
                     /inference="COORDINATES: protein motif:HMM:NF036525.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEX9149430.1"
                     /translation="MPRLKTLDKKQAILDAAARVFSGTQFHEALIDEVAAAARVGKGT
                     IYRYFPTKDDLYFAAVLHSFDELSAALSVTLSRERSPRRRLERIARDVMAFSRNRPDL
                     YVLLQRDERRFPERENELQKRRDTLSRLVQEAILEGIRRREFRGIDARVGAEIFRGMI
                     RAANRLRVGEETL"
     gene            681..890
                     /locus_tag="VF958_09745"
     CDS             681..890
                     /locus_tag="VF958_09745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004464068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="HEX9149431.1"
                     /translation="MAEAVVSTKFQVVIPKEIRREIGLESGQVLQVIAKGGVITLVPD
                     QPIARMMGFLKGMKTSALREKKDRG"
     gene            887..1279
                     /locus_tag="VF958_09750"
     CDS             887..1279
                     /locus_tag="VF958_09750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007294373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HEX9149432.1"
                     /translation="MILVDSSGWIEFYTGGGNAAKYGSYLETAANVLTPTIVLYEVYK
                     LVKRERTEEDALLATAQMQRTRLVPLSESLALAAADVSLEFRLAMADSVVYATARAED
                     AELVTSDSDLRGLPGVTFLPKAGKSRHS"
     gene            1445..1951
                     /locus_tag="VF958_09755"
     CDS             1445..1951
                     /locus_tag="VF958_09755"
                     /inference="COORDINATES: protein motif:HMM:NF017015.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HEX9149433.1"
                     /translation="MEFRSILDEALEAWSYTRAGVIEEARALPEKDFGFRASEKSRSV
                     IELLQHVIESGLLMSGELSRPDGDFRRKSYEGLMIEYARGVSGMRSKRQLLAALRQTH
                     TDGEKRIRAAGELHMLQLINRFDGKKGTRLAWMNHGISHEEYNRGQLAIYARMLGRVP
                     ALTKRIHG"
     gene            complement(1994..2500)
                     /locus_tag="VF958_09760"
     CDS             complement(1994..2500)
                     /locus_tag="VF958_09760"
                     /inference="COORDINATES: protein motif:HMM:NF039330.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6036 family nucleotidyltransferase"
                     /protein_id="HEX9149434.1"
                     /translation="MHALAKEADRETRLYFTGGATAVLMGWRGTTIDVDLKFVPESDR
                     LLRAIPDLKEKLQVNVELASPSDFIPQVPGWEERSRFIGKEGKLSFFHYDFYSQALAK
                     IERGHAQDRADVDEMIRRELVEPGRALEYFDKIEPDLYRYPAVDPAAFRRAVRQVLRD
                     STGPAQKS"
     gene            complement(2527..2802)
                     /locus_tag="VF958_09765"
     CDS             complement(2527..2802)
                     /locus_tag="VF958_09765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9149435.1"
                     /translation="MAFDHLPGAELVFEGLEDIRRGVESIPALLVSIGAPRLRQIGLA
                     VPEPVLRDPEHRLYLLLSRSDPNSAHSRYNALVRRLVSFERAAECAR"
     gene            2855..>3352
                     /locus_tag="VF958_09770"
     CDS             2855..>3352
                     /locus_tag="VF958_09770"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011684913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEX9149436.1"
                     /translation="MSLSRGSRLGPYEIEAAVGAGGMGEVYRAKDTRLDRTVAVKVLP
                     SHLAASAEVRQRFEREAKTISQLSHPHICALYDVGNQDGVEFLVMEYLEGQTLSERLV
                     KGPLAFEQVLRSGLEIADALDKAHRQGIVHRDLKPGNVMITKSGVKLLDFGLAKAVAP
                     ATPQQL"
BASE COUNT          616 a         1060 c         1091 g          585 t
ORIGIN      
        1 tccaacgttt cctcccccac ccgaaggcgg ttcgcggcac gaatcattcc ccggaagatc
       61 tcggctccga cgcgcgcgtc gatcccacgg aattcgcgcc tccggatgcc ctcgaggatc
      121 gcctcctgca cgagccgcga aagcgtgtcg cgtcgcttct gcaattcatt ttcgcgttcg
      181 ggaaaccgcc gttcgtcacg ctgtaacagc acgtacaggt ccggccggtt ccgggaaaag
      241 gccatgacgt cccgggcgat ccgttccagc cggcgccgcg gagaccgttc ccgcgaaagg
      301 gtgacggaga gcgccgccga gagctcgtcg aagctgtgga ggaccgcggc gaagtagagg
      361 tcgtccttcg tggggaagta gcggtagatc gttcccttgc cgaccctggc ggccgccgcg
      421 acctcgtcga tgagcgcttc gtggaactgg gtgcccgaga agacccgggc cgcggcgtcc
      481 aggatcgcct gcttcttgtc gagcgtcttc agtcggggca tcgatatttc agaactgacc
      541 ggtcagttcc attatctcaa tacgtggccg ggagcgagcg ggtttcagtc gaacggccgt
      601 gtcatccgtc gggtctcttc gacctacgaa attgctatcg atccacaaga cgtaagataa
      661 actcgtatcg gaggctttcg atggcggagg cagtcgtctc gacgaaattt caggtcgtca
      721 tcccgaagga gatccgccgg gagatcgggc tcgagtcggg acaggtcctt caggtcatcg
      781 ccaagggcgg cgtgatcacg ctggtccccg atcagccgat cgcacggatg atgggattcc
      841 tgaaaggaat gaagacatcc gcgctacggg aaaagaagga ccgcggatga tcctcgtcga
      901 ttcgtccggt tggatcgagt tctatacggg gggcggaaac gccgcgaagt acgggtccta
      961 cctcgaaacg gccgcgaacg tactgactcc tacgatcgtc ctctacgagg tctacaagct
     1021 ggtgaagcgc gagcgcaccg aggaggatgc tcttctggcg accgcccaga tgcagaggac
     1081 gagactggtt cctctatccg agtcgctggc gctcgcggcg gccgacgtaa gcctggagtt
     1141 ccggctggcc atggcggatt cggtcgtcta cgccacggct cgcgccgagg acgccgaact
     1201 cgtgacgagc gacagcgacc tgcggggcct gcctggagtc acgtttcttc cgaaagctgg
     1261 aaagagccgc cactcatgac gttgtcttca ggcacgcgtc tcggtccgta cgagctcatc
     1321 gcgccggtgg cggggcttct gttgaaccag gtcgccggca tccgcaaata ggctgtttcg
     1381 tggcgggttt tcgccgcccg gatatcattc tcccgatccc gcgactttcg gaggcgaaaa
     1441 aagtatggaa ttccgcagca tcctcgacga agccctggag gcctggtcgt acacgcgcgc
     1501 cggcgtcatc gaagaggccc gcgccctccc cgagaaggac ttcggctttc gtgcgtcgga
     1561 gaagagccgc tcggtgatcg agctcctgca gcacgtcatc gagtcgggac tcctcatgtc
     1621 cggcgagctt tcgcgccccg acggggactt ccgacgcaag tcctacgaag ggctgatgat
     1681 cgagtacgcc cggggcgtct ccgggatgcg ctcgaagcgc cagctcctgg ccgcgctgcg
     1741 gcagacgcac accgacggcg aaaagcggat ccgcgccgcg ggagagctcc acatgctcca
     1801 gctcatcaac cgcttcgacg gaaagaaggg cacacgcctg gcgtggatga atcacgggat
     1861 ctcccacgag gagtacaacc gcgggcagct cgcgatctac gcgcggatgc tcggacgggt
     1921 gccggcgctc acgaagcgaa ttcacggcta gagtcggcgg agatccctcc gaggatcggg
     1981 gtcggcgccg aactcagctt ttctgcgctg gtcccgtgga gtcccggagg acctgccgga
     2041 ccgcgcggcg aaacgccgcc ggatcgacgg ccggatagcg atagagatcc ggttcgatct
     2101 tgtcgaagta ctccagggcg cgccccggtt cgacgagctc tctccggatc atctcgtcca
     2161 cgtcggcacg gtcctgcgca tgccctcgct cgatcttcgc gagcgcctgt gagtagaagt
     2221 cgtagtgaaa gaaggagagc ttcccctcct tcccgatgaa ccggcttcgc tcctcccagc
     2281 cgggaacctg gggaatgaag tccgacggcg acgccagctc gacgtttacc tgaagcttct
     2341 ccttcaggtc aggaatcgcc ctcaggagac gatcggactc cggtacgaat ttcagatcga
     2401 cgtcgatcgt cgtcccgcgc caacccatca gtaccgccgt cgctccgccg gtgaaataga
     2461 gacgagtttc ccggtccgcc tccttcgcga gagcgtgcat caagttcccg attcgaacgg
     2521 cgtcggctag cgcgcgcatt ccgccgcgcg ctcgaagctc accagccgcc gcaccagggc
     2581 gttgtagcgg gagtgggcgg agttcggatc gctgcgcgag agcaggagat acagacggtg
     2641 ttcgggatct cgcaacaccg gttccggcac ggccagaccg atttgccgca atcgtggcgc
     2701 cccgatcgag acgagcagag cgggaatcga ctccactccg cgccgaatgt cctcgagacc
     2761 ctcgaagacc agctcggccc cggggaggtg atcgaatgcc acgattcaat gatagcggcc
     2821 cgggactcgc cgcatatcat tcgtccaatc cggcatgagt ctctcgaggg gcagccgtct
     2881 cggaccttac gagatcgagg ccgcggtcgg cgcgggcggg atgggggagg tttaccgggc
     2941 gaaggacacg cggctcgacc gaacggttgc cgtgaaggtc ctgccgtcgc acctcgccgc
     3001 gagcgcggag gtccgccagc gcttcgagcg agaggcgaag acgatctcgc agctctcgca
     3061 tccccacatc tgtgcgcttt acgacgtcgg caaccaggac ggcgtcgagt tcctggtcat
     3121 ggagtaccta gagggccaga ccctctcgga gcggttggtg aagggcccgc tcgcgttcga
     3181 gcaggtgctg cgctccggcc tcgagatcgc cgacgcgctg gacaaggcgc accggcaggg
     3241 gatcgtccat cgggacctga agccgggtaa cgtgatgatc acgaagtcgg gggtcaagct
     3301 tctcgacttc gggctagcga aggcagtggc gccggcgacg ccgcagcagc tg
//
LOCUS       DASWAV010000470         2732 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U2665
            k141_665442, whole genome shotgun sequence.
ACCESSION   DASWAV010000470 DASWAV010000000
VERSION     DASWAV010000470.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960316
            Sequence Read Archive: SRR10260007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2732)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2732)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:47:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,929
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2732
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_665442"
                     /isolate="SMAG_U2665"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.36444 N 132.31889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            9..83
                     /locus_tag="VF958_09775"
     tRNA            9..83
                     /locus_tag="VF958_09775"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:41..43,aa:Pro,seq:tgg)
     gene            148..1593
                     /locus_tag="VF958_09780"
     CDS             148..1593
                     /locus_tag="VF958_09780"
                     /inference="COORDINATES: protein motif:HMM:NF025253.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0098024 - virus tail, fiber [Evidence
                     IEA];
                     GO_process: GO:0019062 - virion attachment to host cell
                     [Evidence IEA];
                     GO_process: GO:0046718 - viral entry into host cell
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber domain-containing protein"
                     /protein_id="HEX9149437.1"
                     /translation="MRNQRSSPFLLFIGLVVVAGAGTTPLIGQGVANWEAPPFWSPPE
                     PQRPAGQAGTLAATTPLPFIAVTPCRVVDTRGNGFTGDYGPPALSGNGPPRSFAIPAG
                     PCSGIPGNAGAYSINVAAILPAADGFLTVFPTGAAQPASSDLNFLAGEVVANAIVAPA
                     GSGGAISVYVNVSTHMILDINGYYAAASTGIRNTFLGFQAGNPAMTGEDNTGLGLIAL
                     SQNTTGGSNIAVGTFTLPDNTIGSSNTALGGGALRFNGMHSSNTGVGVGAQGAKSLGG
                     QNTAAGYSALVNGTGTTNTALGFGACSSVSKAAGNNICIGSPGVSGDSNTIRIGSGQT
                     ATFVAGINGQTTPGGVLLLVNSDGKLGTTTSSRRFKEDIQEIAGKSDGLMRLRPVAFT
                     YKRELDPTGLVQYGLIAEEVAEVYPDLVAYGKDGQPEAVRYQLINALLLNERQKQHRM
                     IEELESRLAKLEALLTDEAPVGSLLLVEEKP"
     gene            1590..>2732
                     /locus_tag="VF958_09785"
     CDS             1590..>2732
                     /locus_tag="VF958_09785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9149438.1"
                     /translation="MKSHHVLPFLCLFGVLDAALAQTIPNWPAPAFWQPPAPQRVPGQ
                     VSLLAATSPLPFIAITPCRVVDTRNAVGPYGGPALAGNGPARNFNIPGGPCPGIPSFA
                     GAYSINVAAVLPVADGFMTVFPTGTAQPNSSDLNFLGGEVIANALVVPNGSGDSISVF
                     VNVSTHMILDINGYYAVSGVGFRNTFLGVGAGNFTMTGEDNTGIGYQALNFNNAGAVN
                     TGVGGYALENNFSGNANTAVGHVAMRFNETGSSNTAIGYSALTFNTAGGQNTAIGYEA
                     LLSVSGSTNTALGYRAGANLSTGSNNICVGNLGVTGESNTLRIGTSQTATFVAGISGQ
                     TTPGGVPVGVNSSGKLGTTVSSRRFKENIREIGGESDGLMRLHPVAF"
BASE COUNT          474 a          959 c          845 g          454 t
ORIGIN      
        1 cctccttccg gggcgtagcg cagcggtagc gtatctgctt tgggagcaga tggtcgccgg
       61 ttcgatcccg gccgccccga ccagtctttt ccgggtcgtt ttctcgccgg acccgaatcg
      121 atctcggttc gacaaggagg aggcgtcatg aggaaccaac ggagttctcc gtttctcctt
      181 ttcatcggcc tcgtcgtagt ggccggggcc gggacgaccc cgctcattgg gcaaggcgtt
      241 gcgaactggg aggcgccgcc gttctggagc cccccggagc cgcagcgccc ggcgggtcag
      301 gcgggcacgc tcgcagccac cacgccgctc ccgttcatcg cggtcacccc gtgccgcgtc
      361 gtcgacaccc gcggcaacgg cttcacgggc gactacgggc cgcccgccct ctcgggcaac
      421 ggaccgccgc ggtcgttcgc cataccggcc gggccctgtt ccggcatccc gggcaacgcg
      481 ggcgcttact ccataaacgt cgccgcgatc ctccccgccg ccgacgggtt tctgaccgtc
      541 ttccccactg gagccgcgca gcccgcctcc tcggacctga acttcctcgc gggcgaggtc
      601 gtcgccaacg ccatcgtcgc gccggccggc tcgggcggcg cgatcagcgt ctacgtcaac
      661 gtctcgacgc acatgatcct cgacatcaac gggtactacg ccgcagcgtc gacgggcatc
      721 cggaacacgt tcctcggttt ccaggcgggc aaccccgcga tgacgggcga ggacaacacc
      781 ggcctcggac tcatcgcgct ctcccagaac accacgggcg gctccaacat cgccgtcgga
      841 actttcacgc tcccggacaa caccatcggt agctccaaca cggccttggg cggcggcgcg
      901 ctccggttca acggcatgca cagctccaac accggcgttg gagtaggcgc gcagggagcc
      961 aagagcctgg gcggacaaaa caccgccgcc ggatacagtg cgctcgtcaa cgggacggga
     1021 accacgaaca ccgcgctggg cttcggggcc tgcagttcgg tctcgaaggc agcgggcaac
     1081 aacatctgca tcgggagtcc cggcgtttcc ggagactcga acacgatccg gatcggatcg
     1141 ggccagaccg cgaccttcgt ggccggcatc aacggacaga ccaccccggg cggagttctc
     1201 ctcctggtca actcggacgg gaagctcggt acgacgacct cctcccggcg cttcaaggag
     1261 gacatccagg agatcgccgg aaagagcgac ggcctgatga gactgaggcc ggttgcattc
     1321 acgtacaagc gtgagctcga tccgaccgga ctcgtccagt atggcctcat cgccgaggag
     1381 gtggccgagg tgtatccgga cctggtcgcc tatggcaagg acggccaacc cgaggcggtg
     1441 cgatatcaac tgatcaacgc actgctgttg aacgagcgtc agaagcaaca ccggatgatc
     1501 gaggagctcg aatcccgact ggcgaagctc gaggccttgc tcacggacga agcgccggtc
     1561 ggctcccttt tgctcgtgga ggaaaagcca tgaagagtca tcatgttctc ccgtttctct
     1621 gtcttttcgg cgtgctcgac gcggctctgg cacagacgat tccgaactgg ccggcgcccg
     1681 ccttctggca gccgcccgcg ccgcagcgcg tgccgggcca ggtgagcctt ctcgccgcca
     1741 cttcgccgct cccgttcatc gcgatcactc cgtgtcgagt cgtggacacg cgcaatgccg
     1801 tcggccccta cggtggcccc gccctcgccg gcaacgggcc agcgcgcaat ttcaacattc
     1861 cggggggccc ctgcccgggc attccctctt tcgccggtgc ctactcgatc aacgtcgcgg
     1921 cggtcctgcc ggtggccgac gggttcatga ccgtctttcc cacggggacc gctcagccca
     1981 actcttcgga cctgaacttc ctcggcggtg aagtcatcgc caacgccctc gtcgttccga
     2041 acggttcggg cgactcgatc agcgtcttcg tcaacgtctc gacgcatatg atcctcgaca
     2101 tcaacggtta ctacgccgtt tccggggtgg gttttcgaaa cacgtttctc ggggtgggcg
     2161 ccggcaactt cacgatgacg ggtgaggaca acaccggtat cggataccag gcgctcaact
     2221 tcaacaacgc cggtgccgtc aacacaggcg tcggaggtta tgcgctcgaa aacaacttct
     2281 ccggcaacgc caacaccgcc gtggggcatg tcgcgatgcg gttcaacgag accggcagct
     2341 ccaacacggc catcggctac tcggccctga ccttcaacac cgcgggtggc cagaacacgg
     2401 ccatcgggta cgaggcgctg ttgagcgttt ccggctcgac caacaccgcg ctcggctacc
     2461 gggccggggc caacctctcg accggcagca acaacatctg cgtcggaaac ctcggcgtca
     2521 cgggcgagtc gaacacgctc cggatcggga cgtcgcagac ggcgacgttc gtggccggca
     2581 tcagcgggca gaccaccccg ggcggagttc ccgtcggggt caattcatcc ggcaagctcg
     2641 gcacgacagt ttcctcccgc cgcttcaagg aaaacatccg cgagatcggc ggggagagcg
     2701 acggcttgat gaggctgcac cccgtcgcgt tc
//
LOCUS       DASWAV010000471         2546 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U2665
            k141_106172, whole genome shotgun sequence.
ACCESSION   DASWAV010000471 DASWAV010000000
VERSION     DASWAV010000471.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960316
            Sequence Read Archive: SRR10260007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2546)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2546)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:47:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,929
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2546
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_106172"
                     /isolate="SMAG_U2665"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.36444 N 132.31889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1005)
                     /locus_tag="VF958_09790"
     CDS             complement(<1..1005)
                     /locus_tag="VF958_09790"
                     /inference="COORDINATES: protein motif:HMM:TIGR00423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CofH family radical SAM protein"
                     /protein_id="HEX9149439.1"
                     /translation="MTGSSLLAATFRGSDLAPIAEKVLAGERLSFDDGVRLSTTRDFL
                     GLGRLANSAREKLHGNAAFYNVNRYLNPTNLCWVDCALCAWAKKVNEPGGYELAIDEC
                     VAEAGRGYSEAVTEFHIVGGLHPTWPYEYYTGLLRALKEKFPGVHLKAFTMVELDWIA
                     HVGGRTVAEAIRDLKEAGMDSCPGGGAEIFERRVRDVICKNKISGERWLEVARECHLQ
                     GIRTNATILYGHVETAEDRVDHLIRLRDLQDETGGLTGLIPLAFHPENTPLAHLPATS
                     GQLDLRVIAVSRLLLDNVPHVKAYWVQIGTKLAQVALFFGADDLVGTVVDETITHAAG
                     A"
     gene            complement(1002..1220)
                     /locus_tag="VF958_09795"
     CDS             complement(1002..1220)
                     /locus_tag="VF958_09795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9149440.1"
                     /translation="MGPVWRLVRGEKDVCHRCGQEIVDFPAVGHVVDFQFRPEQKYCW
                     ECYRYLQPFVDELGLNSPYQPVTKPAIP"
     gene            complement(1237..1413)
                     /locus_tag="VF958_09800"
     CDS             complement(1237..1413)
                     /locus_tag="VF958_09800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9149441.1"
                     /translation="MKKTRKRKKKATKRGGPPAPARPTARIPLPKKGEKRHGDRTKYE
                     RARERERLRRELGR"
     gene            complement(1410..>2546)
                     /locus_tag="VF958_09805"
     CDS             complement(1410..>2546)
                     /locus_tag="VF958_09805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004513936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX9149442.1"
                     /translation="DVASDMIYPLLPAFLTKTLGAGPATVGLIEGVAETTASLAKIGS
                     GTWSDRVRRRKPLVVLGYVIAAVARPLVAFARVWTQVLAIRFTDRVGKGIRTSPRDAL
                     LADIVPPQRRGRAFGLQRTMDNAGAFVGPLVAALLLKLAVVDERGVFLLAAIPGIAAV
                     VLLLVAVPDIPKRRETHPAATGTKAALPRAFWKIVAVFAFFTLANSTDAFLLLRAQGA
                     GVATWQLPLLWSFFQGVKAATGVPGGVVADRAGRVPAITLGWFVYAAAYAGFAFVARP
                     LAVWGLVALYALFYGLTEGAERALVADLVPENLRGRAYGIFHAAIGIAALPASVVFGL
                     LWNRFSPRVAFLTGAVVALLAALALWAVAATIRRDGARARSAAA"
BASE COUNT          417 a          860 c          883 g          386 t
ORIGIN      
        1 cgcgccggcg gcgtgcgtga tggtttcgtc gacgaccgta ccgacgaggt cgtccgcgcc
       61 gaagaagagc gcgacctgcg cgagcttcgt cccgatctga acccagtacg ccttgacgtg
      121 cgggacgttg tcgagaagga gtcgcgagac cgcgatcacc cgaaggtcga gctggcccga
      181 cgtcgccgga aggtgcgcga gcggcgtgtt ttcggggtga aaggcgaggg ggatcaaccc
      241 cgtgaggccg cccgtctcgt cctgcaggtc gcgcaggcgg atcaggtggt ccacgcggtc
      301 ttccgcggtc tcgacgtgtc cgtacaggat cgtcgcgttc gtgcggatcc cctgcaggtg
      361 gcactcgcgc gcgacctcga gccagcgctc accggagatc ttgttcttgc agatcacgtc
      421 gcgaacgcgc ctctcgaaga tctccgctcc accgccgggg caggagtcca tcccggcctc
      481 cttgaggtcg cggattgcct cggcgaccgt tcgtcccccg acgtgcgcga tccagtcgag
      541 ctccaccatc gtgaaggcct tcaagtggac gccggggaac ttctccttca atgcgcgaag
      601 aagccccgtg tagtactcgt agggccaggt cgggtggaga ccccccacga tgtggaactc
      661 cgtcacggct tcgctgtagc cgcgccccgc ctccgcgacg cactcgtcga tcgcgagctc
      721 gtagcccccg ggctcgttca ccttcttcgc ccaggcgcag agggcgcagt cgacccagca
      781 gagattggtg ggattgaggt atcggttgac gttgtagaac gcggcgttcc cgtggagctt
      841 ctcccgagcg gagttcgcca ggcgcccgag gccgaggaaa tcccgggtcg tcgacaggcg
      901 gacgccgtcg tcgaaggaca gcctttcgcc ggcgagaacc ttttcggcga tcggagcgag
      961 gtcggagccg cggaacgtcg cagcgaggag ggacgaaccg gtcacgggat ggccggcttc
     1021 gtcaccggct ggtacgggct gttcaagccg agctcgtcga cgaagggctg gaggtagcgg
     1081 tagcactccc agcagtactt ctgctccggc cggaactgga agtccaccac gtgtcccacc
     1141 gcggggaagt cgacgatctc ctggccgcag cggtggcaga cgtctttctc tccccggacg
     1201 agcctccaga cgggtcccat gccacgaacg atcgtatcag cgcccgagct cccggcgcag
     1261 ccgctccctc tcccgggcac gctcgtactt cgtgcggtcg ccgtggcgct tctccccctt
     1321 tttcgggagc gggatgcggg cggtcggccg ggccggcgcc ggggggcctc cgcgcttcgt
     1381 agccttcttc ttcctcttcc gcgttttctt catgctgccg ccgatcgtgc tctcgctccg
     1441 tcgcggcgga tcgtcgccgc gaccgcccac agggcgagag cggcgaggag agcgacgacg
     1501 gctccggtca agaaagcgac gcgtgggctg aagcggttcc agagaagacc gaagacgacg
     1561 gatgcgggca gggccgcgat tccgatcgcc gcgtggaaga ttccgtacgc ccgtccccgc
     1621 aagttttccg gcacgaggtc ggcgacgagc gcccgctcgg cgccctccgt caaaccgtag
     1681 aagagcgcgt aaagggcgac gagaccccac accgccagcg ggcgggcgac gaatgcgaaa
     1741 cccgcgtatg cggccgcgta gacgaaccag ccgagagtga tggccggcac gcgaccagcg
     1801 cgatcggcga cgacgccccc gggaactccg gtcgccgcct tcaccccctg gaaaaacgac
     1861 cagaggaggg gcagctgcca cgtcgcgact cccgcgccct gcgcgcgcag caggaggaag
     1921 gcgtccgtcg agttcgccag cgtgaagaac gcgaataccg cgacgatctt ccagaacgcg
     1981 cggggaagcg ccgccttcgt cccggtggcc gccggatgcg tctctcttcg cttcgggatg
     2041 tcggggacgg cgacgagaag gaggacgacc gccgcgatgc cggggatcgc ggcgagcaga
     2101 aagactccgc gctcgtcgac gaccgcgagc ttgagaagga gcgcggcgac gagcggcccg
     2161 acgaacgcgc cggcgttgtc catcgtccgc tgcagcccga aggcgcggcc gcgcctttgc
     2221 ggcgggacga tgtcggcgag cagtgcatcc ctcggcgagg tccggatgcc ctttccaacc
     2281 cggtctgtga aacggatcgc gagcacctgc gtccatacgc gagcgaaagc gacgagcggc
     2341 cgcgcgaccg ccgcgatcac gtaaccgagg accacgagcg gtttccggcg gcggacccgg
     2401 tccgaccagg ttccggagcc gatcttggcg agggaagcgg tcgtctctgc gacgccctcg
     2461 atcaacccga ccgtggccgg gcccgctccg agcgtcttcg tcaggaaggc cggaaggagc
     2521 gggtagatca tgtcggaggc cacgtc
//
LOCUS       DASWAV010000472         1703 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U2665
            k141_506620, whole genome shotgun sequence.
ACCESSION   DASWAV010000472 DASWAV010000000
VERSION     DASWAV010000472.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960316
            Sequence Read Archive: SRR10260007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 1703)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1703)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:47:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,929
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1703
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_506620"
                     /isolate="SMAG_U2665"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.36444 N 132.31889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..301)
                     /locus_tag="VF958_09810"
     CDS             complement(<1..301)
                     /locus_tag="VF958_09810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9149443.1"
                     /translation="MKRFSVVPPIPYSLALTAQRYTRFADPVDHFDGKTYGRLLSVGR
                     GTLLVAVEQSGGATNPKLRVTLEGPGEDSPRARAAAERLVRGALGAGTDVRLFSRA"
     gene            676..1146
                     /locus_tag="VF958_09815"
     CDS             676..1146
                     /locus_tag="VF958_09815"
                     /inference="COORDINATES: protein motif:HMM:NF025173.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="periplasmic heavy metal sensor"
                     /protein_id="HEX9149444.1"
                     /translation="MKRFAIRQLAPVAAVLFTLGVALASAQDGPHRHGKMGAFGMHAL
                     SRLDLSEAQKADVQRIMDSKKATFQSLREQMRADWQALDAAADAQSPSTSEVGAAFLK
                     VRSDRQAMRAEHQATMEQIRAILTPDQKDKLDTMRQKRQERFQGRGGMRERFGR"
     gene            1287..>1703
                     /locus_tag="VF958_09820"
     CDS             1287..>1703
                     /locus_tag="VF958_09820"
                     /inference="COORDINATES: protein motif:HMM:NF019247.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1579 family protein"
                     /protein_id="HEX9149445.1"
                     /translation="MKLRGILAGLAFLIVIAALAIGADEKGKKTKPAAGKMDEKAMME
                     MMAKYSTPGAEHKKLEAFVGTWDTTAKMWMEPGAPPQESTGVSENKMALGGRYLEQNF
                     EGTMMNQPFSGRGYTGCDLYKKQYFGTWMDTMGTSMM"
BASE COUNT          330 a          552 c          567 g          254 t
ORIGIN      
        1 ccgcccggga gaagagtcgc acgtcggtcc cggcaccgag cgcgcccctc accagtcgct
       61 cggctgcggc gcgcgcgcgg ggggagtcct ctccgggacc ttccaacgtg acccgaagct
      121 tcgggttcgt cgcgccccca ctctgctcga ccgcgacgag cagagtcccg cgcccgaccg
      181 agaggagccg gccgtacgtt ttcccgtcga agtggtcgac ggggtcggca aacctcgtgt
      241 accgctgcgc cgtcaacgcg agcgagtacg ggatgggcgg tacgacagag aagcgcttca
      301 ccgcgagtcc gcgatccgcc gcaagagcgg cgagtcgtcc gtttttcgat tgccgagaac
      361 cgtcaaggag cgcggcgagg atacatctcc gtcgcgttcg cttgctcggg acctggctcc
      421 gccatcggcg agcggctcgc caaccccggc tcgaccgctc gcctggtccc tccctcgctc
      481 gcgctcccgc ggcgcgaggc tcctccctcg attacctcgg gagactcctc gcgccgcgag
      541 gaatcggaag agtccggaga ctcctcgcgc cgcgattggg ttgccggaat ccacgaggcg
      601 actcgttcgt ggttgtgggc ggtaaacgaa acggcgactc cgttcgtcaa ttggttcgta
      661 aaggagactc aagccatgaa gagattcgcg attcgacagc tcgcccccgt ggcggcggtg
      721 ctgttcacgc tgggggtcgc tctggcttcc gcacaggacg gtccccaccg tcacggaaag
      781 atgggcgcgt tcggaatgca cgcgctgtcg cgcctggatc tgtccgaagc ccagaaggcc
      841 gacgtccagc gcatcatgga ctcgaagaaa gcgacgttcc aatcgctccg cgagcagatg
      901 cgcgccgact ggcaggcgct cgacgcggcg gccgacgcgc agtcgccgag cacttccgag
      961 gtcggagcgg cgttcctgaa ggttcgttcg gaccgccagg ccatgcgcgc cgaacaccag
     1021 gcgaccatgg aacagatccg cgcgatcctg acccccgacc agaaggacaa gctcgatacg
     1081 atgaggcaga agcgccagga gcgcttccag gggcggggcg ggatgcggga gcgtttcggc
     1141 cgctaactcc tcgaatccac acggtaagcc agccaggggc tcgaaggagt ccctgatttt
     1201 tttagccggc cgccccgcat agaatccccc cgcagagctc ccgactcttc gaaaaagtcc
     1261 cgcatgggac caaaggagga accagcatga aacttcgggg aatcctggcc ggcctcgcct
     1321 ttctgatcgt catcgccgcg ctcgccatcg gtgcggacga gaagggcaaa aagaccaagc
     1381 cggccgccgg caagatggac gagaaagcca tgatggagat gatggcgaag tactcgacgc
     1441 ccggcgccga gcacaagaaa ctcgaggcat tcgtcgggac gtgggacacg accgccaaga
     1501 tgtggatgga gccgggcgcg ccgccgcagg agagcacggg cgtttcggag aacaagatgg
     1561 cgctcggcgg ccggtacctc gaacagaact tcgaggggac gatgatgaac cagcccttca
     1621 gcggcagggg ctacacgggc tgcgacctgt acaagaagca gtacttcgga acgtggatgg
     1681 acacgatggg tacgagcatg atg
//
LOCUS       DASWAV010000473         4123 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U2665
            k141_148132, whole genome shotgun sequence.
ACCESSION   DASWAV010000473 DASWAV010000000
VERSION     DASWAV010000473.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960316
            Sequence Read Archive: SRR10260007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 4123)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4123)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:47:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,929
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4123
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_148132"
                     /isolate="SMAG_U2665"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.36444 N 132.31889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1146)
                     /locus_tag="VF958_09825"
     CDS             complement(<1..1146)
                     /locus_tag="VF958_09825"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010034708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HEX9149446.1"
                     /translation="MIALVLVAALARPADAGFSAARLDRIDGVVAEAIARGELPGAVV
                     LVGRRDRIVFRRAYGSRAVLPSREPMAVDTVFDVASLTKPVATATSVLILVERGRLAL
                     GDPVVKYIPEFAPDGGDREKVTVEQLLTHRAGLAPDDPIDLYTGTPEEIFARKYRQPL
                     ESPPGTRFRYSDVGYEVLGDLVRRVSGLPLDEFSEREIFRPLGMKDTHFRPLATTRFL
                     GDRIGLADPERPPISRIAPTERRDDHWLRGEVHDPRAFVVGGVAGHAGLFSTADDLSR
                     WCRMILAGGRLGSTRVLSPPGVEAMTRPRFFGDGDLRALAFDVATAYSRNRGDLFPPG
                     SFGHTGFTGTSLWIDPSSGVFVVFLSSRLHPDGKGDVNRLRGLVATIV"
     gene            complement(1143..2948)
                     /locus_tag="VF958_09830"
     CDS             complement(1143..2948)
                     /locus_tag="VF958_09830"
                     /inference="COORDINATES: protein motif:HMM:NF012686.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter family protein"
                     /protein_id="HEX9149447.1"
                     /translation="MTLGTVDILVLGAYFAMTLAVGLFFARRARRSMLDYFLSARSMP
                     WWVAGTAMVATTFSADTPLAVTEMVAKNGVAGNWLWWSMLASGMLTVFFFAKLWRRAA
                     VVTDVELAELRYSGRPAAFLRGFRAAYLGLLVNVLIMGWVNLGMAKVLGGMLGVSKWA
                     ALVICLALTALYTVMSGYWGVASANGFQYLFEMGGAIVLAFVSVGAVGGIESLKARLG
                     AAHPAFAAPGVRFGSAGAILSVWPPSGDAAWVLPAITFATLLGVSWWASWYPGAEPGG
                     GGYVAQNMLACKDERNARAAALYFNVAHYAIRSWPWVVTALCSLAIYGGSIRGASGVE
                     DPGLNYVKMMVDFLPAGLRGFMLASFAAAYMSTQATQMNWGSSYLVNDLYRRFIRRQA
                     PERHYVLVSRAATALTVVLSLVATISMSQISKVWEFLLTLGAGTGLVYILRWYWWRVN
                     AWSEVAAMASALVTSLGLRSLGSRYPAFDPATPKGFAMTLVTTTAVTTVVWLAATFGT
                     RAEPREKLVAFYEKVGPAGPGWRRIVGAERVAEHRGEIPRNLLFWLLGTAFVYSLMFA
                     TGAVIFHETSRALIFGAVLAISGGLLFTGLVREKT"
     gene            complement(2945..>4123)
                     /locus_tag="VF958_09835"
     CDS             complement(2945..>4123)
                     /locus_tag="VF958_09835"
                     /inference="COORDINATES: protein motif:HMM:NF013126.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 N-terminal
                     domain-containing protein"
                     /protein_id="HEX9149448.1"
                     /translation="TAKHFPGHGDTRTDSHRSLPVLAVDRSRLERVELVPFRAAIAAG
                     VGAVMIAHLSLPAIDSTTVPLREVAPGENPYTDDRAEIAPDATMPASMSAAVVEGLLR
                     RDLGFRGLVVADALDMGAIVDHFDPGEAAVRAILAGADQVIKSADTDAAIAGVRRAVA
                     EGRISMERLDRSVANVLNAKRRFPAAIPDPEATFRAVDGPEHRALAEEIARRAVTLVR
                     EAPGTLPLSRSTRLVHVVVSDVPVASPPGAELASALSARLAAPTRVFLLDPKSRAEDV
                     ESVREAAVDADAVLLSLFVRVRTGTGKLVLPDAARRAVEILSAAGAKPIGVSFGNPYL
                     AADLPGLATYVAAYGDQPVMQAAVARALFGETEISGRLPVTIPGIAERGNGIVRGGTP
                     "
BASE COUNT          720 a         1416 c         1457 g          530 t
ORIGIN      
        1 gacgattgtc gcgacgagcc cccgcagccg gttgacgtcg cccttgccgt cggggtgcag
       61 ccggctcgag aggaacacga cgaagacccc gctggaggga tcgatccaga gagacgttcc
      121 ggtgaagccg gtgtgtccga aggagccggg cgggaagagg tcgccgcggt tgcgcgagta
      181 cgcggtcgcg acgtcgaacg cgagcgcccg cagatcgccg tcgccgaaga accggggccg
      241 tgtcatcgcc tcgacacccg ggggcgaaag gactcgcgtc gagcccagtc tcccgcccgc
      301 gaggatcatt cggcaccagc gcgagaggtc gtccgctgtc gagaaaagtc ccgcgtgccc
      361 ggcgacgccg ccgacgacaa aagcccgcgg gtcgtggacc tcgccgcgga gccagtggtc
      421 gtcgcgcctc tccgtcggtg cgatccgtga gatcgggggg cgctccgggt ccgcgagccc
      481 gattcggtct ccgagaaacc gtgtcgttgc gagcgggcgg aaatgcgtgt ctttcatccc
      541 gagcggccga aagatctcgc gctcggaaaa ctcgtcgagc ggcagtccgg acactcggcg
      601 cacgaggtcg cccaacactt cgtagccgac gtcggagtac cggaaacggg tcccgggcgg
      661 cgactcgagg ggttggcgat acttccgggc gaagatctcc tcgggcgtgc cggtgtagag
      721 gtcgatcgga tcgtcgggcg cgaggcccgc ccggtgcgtg agcagctgct cgacggtgac
      781 cttttcgcgg tcgcctccgt ccggagcgaa ctccgggatg tacttcacga ccgggtcgcc
      841 gagagcgagc cgcccgcgct cgacgaggat caggaccgag gtcgcggtcg ccacgggctt
      901 cgtgagcgac gccacgtcga agaccgtatc cacggccatc ggctcccggg acgggaggac
      961 cgcccggctt ccgtaggccc ttcgaaagac gatccggtcg cggcggccca cgagcacgac
     1021 ggcgccggga agctcgccgc gcgcgattgc ctccgcgacg acgccgtcga tgcggtcgag
     1081 ccgcgccgcc gagaacccgg cgtccgcggg gcgcgcgagc gccgcgacca gcaccagagc
     1141 gatcatgtct tctcgcggac gaggcccgtg aaaagaaggc ctcccgagat cgccagcacg
     1201 gctccgaaaa tgagcgcccg cgacgtctcg tggaagatta cggcgcccgt cgcgaacatg
     1261 agcgagtaga cgaacgcggt cccgaggagc cagaagagga ggttgcgagg gatctcgccc
     1321 cggtgctcgg ccacacgctc ggctccgaca atccgcctcc agccgggacc ggcgggaccg
     1381 actttctcgt agaacgcgac gagcttttcg cgcggttccg cgcgcgttcc gaacgtcgca
     1441 gcgagccaca cgaccgtcgt gactgcggtc gtcgtcacga gcgtcatcgc gaatcccttc
     1501 ggcgtcgccg ggtcgaacgc cggataccgt gagccgagcg aacgcagtcc cagggaagtc
     1561 acgagggcgg acgccatcgc ggcgacctcc gaccacgcgt tcacgcgcca ccagtaccag
     1621 cgcaggatgt agacgagccc ggtcccggcg ccgagcgtca ggaggaactc ccacaccttg
     1681 ctgatctgac tcatggagat cgtcgcgacg agcgacagga cgaccgtgag cgcggtcgcg
     1741 gcgcgggaga cgaggacgta gtgtcgctcc ggagcctgcc gccggatgaa tctccggtag
     1801 agatcgttga cgagataact cgagccccag ttcatctgcg ttgcctgcgt cgacatgtac
     1861 gcggccgcga acgacgcgag catgaacccg cgcaggccgg cggggaggaa gtcgaccatc
     1921 atcttcacgt agttcaggcc gggatcttcg acgcccgacg cgccgcggat cgatccgccg
     1981 tagatcgcga gcgagcagag agccgtgacg acccacggcc agctccggat cgcgtagtgg
     2041 gcgacgttga aatagagcgc agcggcacgg gcgttgcgct cgtccttgca cgcgagcatg
     2101 ttctgcgcga cgtacccgcc gcctccgggc tccgcgccgg ggtaccacga cgcccaccag
     2161 gagacgccga gaagggtcgc gaacgtgatg gccgggagga cccacgccgc gtcgcccgac
     2221 ggcggccaga ccgagaggat cgcgcccgcg gacccgaagc gcacgccggg tgcggcgaag
     2281 gccgggtgcg ccgcgccgag cctcgccttc agagactcga tcccgccgac cgcgccgacc
     2341 gacacgaagg cgagcacgat cgcgccgccc atctcgaaga gatactggaa accgttcgcc
     2401 gacgcgactc cccagtagcc ggacatcacg gtgtagagcg cggtcagggc gaggcagatc
     2461 accagcgccg cccacttcga tactcccagc atgccgccga gcacttttgc catccccagg
     2521 ttcacccatc ccatgatgag gacgttcacg agaagtccca gatacgcggc gcggaagccg
     2581 cgcaggaacg ccgcggggcg acccgagtag cgcagctccg ccagctcgac gtcggtgacg
     2641 acggcggcac ggcgccagag cttcgcgaag aagaagaccg tgagcattcc ggaggcgagc
     2701 atcgaccacc agagccagtt cccggcgacg ccgttcttcg ccaccatctc ggtgaccgcg
     2761 agcggcgtgt cggcggagaa cgtcgtcgcg accatcgccg tgccggcgac ccaccatggc
     2821 atcgagcgcg cggagaggaa atagtcgagc atcgagcggc gagcgcgccg ggcgaagaag
     2881 agcccgacgg cgagagtcat cgcgaagtac gcgccgagaa cgaggatgtc gaccgtcccg
     2941 agcgtcacgg tgtcccccct ctcacgatgc cgtttccgcg ctccgcgatc ccggggatcg
     3001 tgaccggcag ccttccggag atctccgtct ccccgaagag agcgcgagcg acggcggcct
     3061 gcatcaccgg ctggtcgccg tacgcggcga cgtaagtcgc gagccccggc aggtcggccg
     3121 cgagataggg attgccgaat gaaacgccga tcggtttcgc tcccgccgcc gacaggatct
     3181 cgacggccct gcgcgcggcg tcgggcagga cgagcttccc cgtccccgtg cgcacccgga
     3241 cgaagagaga gaggagaacg gcgtccgcgt cgaccgcggc ctcccgcaca gactcgacgt
     3301 cttccgcgcg cgacttcggg tcgagaagga acacccgggt cggcgctgcg agccgagcgg
     3361 acagagcgct cgcgagttcc gcgcccggcg ggcttgcgac cggcacgtcg ctcacgacga
     3421 cgtgaacgag gcgcgtcgat cgcgaaagcg gaagcgttcc cggcgcctcg cggacgagcg
     3481 tcaccgcgcg cctcgcgatc tcttcggcaa gggcccggtg ttccggcccg tccacggcgc
     3541 gaaacgtggc ctcgggatcc ggaatcgccg cggggaatcg gcgcttggca ttgaggacgt
     3601 tcgccaccga acggtccagg cgctccatcg agatgcggcc ttcggcgacc gcccggcgca
     3661 cgccggcgat cgcggcgtcg gtgtccgcgg acttgatgac ctgatccgcg ccggcgagga
     3721 tcgcgcggac ggccgcctcg cccggatcga agtggtccac gatcgcgccc atgtcgagag
     3781 cgtcggcgac gacgagaccg cggaagccga ggtcgcggcg gagcaggccc tccacgacgg
     3841 cggcagacat cgacgccggc atcgtcgcat cgggggcgat ctcggcacgg tcgtcggtgt
     3901 aggggttctc gccgggggcg acttcgcgaa gcgggaccgt agtcgaatcg atcgcgggca
     3961 gcgagagatg cgcgatcatg accgcgccga cgccggccgc gatcgcggcg cgaaagggaa
     4021 cgagctccac gcgctccagc cggctccggt ccacggcaag gaccggcagc gatcggtgcg
     4081 aatccgtccg ggtgtcgccg tgaccgggaa agtgcttcgc ggt
//
LOCUS       DASWAV010000474         1806 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U2665
            k141_148286, whole genome shotgun sequence.
ACCESSION   DASWAV010000474 DASWAV010000000
VERSION     DASWAV010000474.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960316
            Sequence Read Archive: SRR10260007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 1806)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1806)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:47:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,929
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1806
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_148286"
                     /isolate="SMAG_U2665"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.36444 N 132.31889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1799)
                     /locus_tag="VF958_09840"
     CDS             complement(<1..1799)
                     /locus_tag="VF958_09840"
                     /inference="COORDINATES: protein motif:HMM:NF019335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HEX9149449.1"
                     /translation="MSGESVVIDPTQTNTQVNLKEDHLKYAAIGLANRSYQNVILEAP
                     GVSTQTGGAGNPSVYGSNLGQNSFLIDGLNTTDPVTHTFTSNFTYDSIQEIAVQTSGF
                     EAEYGKAIGGIINVITKSGGNDFHGTLDARYRSQDLTEQGTRKRDYPPGTDVLRYDKD
                     TQDFHQLSPAATLGGPLLRDRVWFFGSLERPDFTNQRPDLFGFQPGRRDFKGWTIFGK
                     VTATPAPSHTLALKFNSETADIDHAEDSSFSSPEAGYIQKQDLRVYNGSYDAVLTPSW
                     LANVQLGYLENKLDSRPVSGDLDTTGTIDQVTGIATGNYTNIQFSKRPRFQVLGSTSY
                     IADFLGSHVFKAGTDLEWTKFSTVNNVTGTPIDPSFCSEQFGQPAGATCGAINEPADG
                     DPFLYLVYTDVPEQTFKGHGMAFYGQDEWRPIANLTAKLGVRYDEADFFLPGDEKVKT
                     FARFQPRVGVAWDIFNNSSTIVKANAGEFMEDNALTLPSFVSRQGSVLSLFLWSNAQQ
                     RFRFFGAFGGPSGNALDPSLRPTYSQQVSGGVTQRIFRNTSLDVTGIYRRNRNIFEDS
                     CADEDCTHYVLTNRPNGMDVLKSDYRGVIFKVE"
BASE COUNT          292 a          545 c          611 g          358 t
ORIGIN      
        1 ctctcgacct tgaagatcac gccgcggtag tcggacttga ggacgtccat gccgttcgga
       61 cgattcgtga gcacgtagtg ggtgcagtct tcgtccgcgc acgagtcttc gaaaatgttc
      121 cggttgcgcc ggtagattcc ggtgacgtcg aggctcgtgt tccggaagat ccgctgcgta
      181 actcccccgg acacctgctg cgagtaggtc ggccgcaacg agggatcgag cgcgttcccg
      241 gacggtccgc cgaaggcgcc gaagaaacgg aaacgctgct gggcgttgct ccagaggaac
      301 agcgacagga cggaaccctg acgcgacacg aaactcggga gcgtcagggc gttatcctcc
      361 atgaactcgc cagcattcgc cttcacgatc gtggaggagt tgttgaagat gtcccaggcg
      421 acgccgacgc gcggctggaa gcgcgcgaac gtcttcacct tctcgtcgcc gggcagaaag
      481 aagtccgcct cgtcgtaccg cacgccgagc ttggcggtga ggttcgcgat cggccgccac
      541 tcgtcctggc cgtagaacgc catgccgtgg cccttgaacg tctgttcggg cacgtccgtg
      601 tagacgagat agaggaacgg gtccccgtcg gcgggctcgt tgatcgcgcc acaggtggcg
      661 cccgccggct ggccgaactg ctccgagcag aagctcgggt cgatcggcgt tcccgtcacg
      721 ttgttcacgg tgctgaactt cgtccactcg aggtccgtgc cggccttgaa gacgtggctg
      781 ccgaggaaat cggcgatgta actcgtcgat ccgaggacct ggaagcgagg gcgtttggag
      841 aactggatgt tcgtgtagtt gcccgtcgcg atcccggtca cctggtcgat cgtgcccgtc
      901 gtatcgaggt caccgctgac gggccgggag tcgagcttgt tttcgaggta gccgagttga
      961 acgttggcga gccagctcgg ggtcagaacg gcatcgtagg agccgttgta gacccggagg
     1021 tcctgcttct ggatgtatcc cgcctcgggg ctggagaagg agctgtcctc ggcgtgatcg
     1081 atgtcggccg tctcgctgtt gaacttcaga gccagcgtgt ggctcggcgc cggcgtcgcg
     1141 gtgaccttgc cgaagatcgt ccagcccttg aagtcgcgcc ggcccggctg aaagccgaac
     1201 aggtccggcc gctgattggt gaagtccggg cgctcgagcg aaccgaagaa ccacacccgg
     1261 tcgcgcagga gcgggccgcc gagcgtcgcg gccggcgaaa gctggtggaa gtcctgggtg
     1321 tccttgtcgt accggagcac gtccgtcccc ggcgggtagt cgcgcttgcg cgttccctgc
     1381 tcggtcaggt cctgtgagcg gtaccgcgcg tcgagcgttc cgtggaagtc gttcccgccc
     1441 gatttcgtga tgacgttgat gatcccgccg atcgctttcc cgtactccgc ttcgaagccg
     1501 ctcgtctgga ccgcgatctc ctggatggag tcgtaggtga agttcgacgt gaacgtgtgg
     1561 gtgaccgggt cggtcgtgtt caacccgtcg atcagaaacg agttctgtcc caggttcgag
     1621 ccgtagaccg acgggttgcc ggcgcctccg gtctgcgtcg aaacacccgg cgcctcgagg
     1681 atcacgttct ggtaggaacg gttcgcgagg ccgatcgcag cgtacttcaa gtgatcctct
     1741 ttcaggttca cctgcgtgtt ggtctgcgtg gggtcgatga cgacgctttc gccgctcacc
     1801 tcgacc
//
LOCUS       DASWAV010000475         6316 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U2665
            k141_187263, whole genome shotgun sequence.
ACCESSION   DASWAV010000475 DASWAV010000000
VERSION     DASWAV010000475.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960316
            Sequence Read Archive: SRR10260007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 6316)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6316)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:47:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,929
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6316
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_187263"
                     /isolate="SMAG_U2665"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.36444 N 132.31889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..424)
                     /locus_tag="VF958_09845"
     CDS             complement(<1..424)
                     /locus_tag="VF958_09845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="lyase"
                     /protein_id="HEX9149450.1"
                     /translation="MRRRIAPVGLFLLLLPPALGAAEWKVSIREFDVPTASSRPHDPE
                     VAPDGSLWYTGQLANKMGRLDPRTGTIKEFALPKADSGPHGLAAGKDGNIWFTANSAA
                     YIGKLDPLTGKVTEYAMPDSRARDPHSLAFDPEGTIFFT"
     gene            complement(433..1032)
                     /locus_tag="VF958_09850"
     CDS             complement(433..1032)
                     /locus_tag="VF958_09850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017721338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DJ-1/PfpI family protein"
                     /protein_id="HEX9149451.1"
                     /translation="MAGKKILMLVGDFVEDYEVMVPFQALLMVGHTVHAVCPGKKAGE
                     KVRTAVHDFEGDQTYSEKRGHNFSLNFDFDAVREGDYDALVIPGGRAPEYLRLNPRVL
                     EIVKHFAAREKPIASICHGAQILAAAGVVSGRRCSSYPAVGPEVTRAGGTYADIPVDQ
                     AVTDRNLVTAPAWPAHPVWLAQFLHVLGTRVEHAAVVEV"
     gene            1100..2188
                     /locus_tag="VF958_09855"
     CDS             1100..2188
                     /locus_tag="VF958_09855"
                     /inference="COORDINATES: protein motif:HMM:NF024743.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4097 family beta strand repeat-containing
                     protein"
                     /protein_id="HEX9149452.1"
                     /translation="MSSTTLRWLGTLTALVLVPVAARAEVTRTLRAQIPGAASRPVAV
                     ENLLGTTRVTVGTADALVVVATVHAENDALASSVRLDELRGDGGVATLRIRYPAISRV
                     LRYPRRDHEHRWDGLVLFGNGNVERYDGARYRVSSHRGTLLYADVEVQVPARVADATF
                     LETVGELDADGLEGKLSFRVASADLRLDRLNGDVEVTGTSGDTRASRIRGTWRSDFSS
                     GDSEMERFDGSSATFHTSSGDIAVRGIASERLSITTSSGDARVRDADVRELQTDASSG
                     DLSFETAGTRLARVLVRSSSGDVHLALAPEAPFQAEADQSSGEMRVRFDDGERRYRRG
                     DLVAFRRGTAGADIRVTTSSGDFTIDPR"
     gene            2260..3126
                     /locus_tag="VF958_09860"
     CDS             2260..3126
                     /locus_tag="VF958_09860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008833805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NmrA/HSCARG family protein"
                     /protein_id="HEX9149453.1"
                     /translation="MADSNRTVLVTGATGNQGGSVAQELLSRGGYRVRAMTRKPDGEK
                     ARALKDRGAEIVAGDLNDEASLRKAMQGAWGVFAVQNTWEAGVEGEEEQGKRQAKVAR
                     DAGVQHYVYTSVASADRKTGIPHFENKWRIEETVRGLGFPSHVILRPVFFMENLLGPW
                     FKSAIDQGQLAMAVKPETRLQMIAVEDIGKYGRWAFDKQETLNGREIDIAGDELTMPE
                     TARLLSRASGHPVAFAPPPIEAVRQGSADFAAMLEWFDRVGYDADIAANARESGIRPT
                     TFAEWASRQKWS"
     gene            3263..4087
                     /locus_tag="VF958_09865"
     CDS             3263..4087
                     /locus_tag="VF958_09865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP/GTP-binding protein"
                     /protein_id="HEX9149454.1"
                     /translation="MLSPLLLAVSLIATARGFQAPESVFWDEVSRTWFVSNVVGKENE
                     KDGKGWISRLDANGKVVQRKWVEGLDSPHGVRVHAGTLYAADVDRIVVIDVAKARIRE
                     RISAPGAKFLNDVAVGPSGDVYVSDTLGDAIYRCTTSCEVLVKSDALEGPNGLLVDGD
                     RLIVATWGPITDPITFATKTPGRLISVDLKTKQIAPLGEGKPIGNLDGIEKDGADYLV
                     TDFMAGKIFRVSKSVAATVLKSGFAESADLGWDPGRRIIAVPEMGGGTVKFFRLSR"
     gene            4099..>6316
                     /locus_tag="VF958_09870"
     CDS             4099..>6316
                     /locus_tag="VF958_09870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9149455.1"
                     /translation="MKKLLVAGMLAACSLLPAAAQTIDPSLFAEMRWRLVGPFRGGWA
                     TMAEGIPDQPDTFFFGAAGGGVWKTDDAGRTWTPLFDKASTASIGALAIAPTDPKVIY
                     VGTGQPQPRYDVISGDGVFRSDDGGKTWAHCGLEATRHIGRILVDPRDANTVLVGALG
                     HIFGPNRERGVFRSTDGGRTWSPVLFVDESTGVVDLASDPAQPDVVYASAWQFRNYPW
                     LSYFKPNTGPGSGVYRSTDGGRTWKRLSGSGWPTENLGRIGLSVSPGGRVWALVDAQS
                     ALAWLDPTRATTSPAGLYRSDDGGATWSHVNTTPGLASYYMGRVTADPKNSDVVYVMG
                     QSIRRSDDGGKTFRIVKGAPGGDDYHFLWINPKHPDHMVTSADQGTVVTVNGGRTWSD
                     WYNQPTGQFYHVETDDRFPYWIYSGQQDSGTAGTMSRSDFGGLTFRDWHPVGAEERGW
                     DVPDPADPDIVYGTGLGGNVMRFDRRTKQAVNISPVVESTYARRPTEVKYRYTWITPL
                     AVSKKAPHAIYYGSQVLFRSTDRGQSWTVVSPDLTGAKPGTAGCDGEITLANASPCGF
                     GVIFTIALSPSDENEIWVGTDDGRIQATRDGGKSWNDVTPKNLPAWSKVSSIDVSALE
                     TGTAYAAIDTHRLDDFSPHLLRTHDGGKTWTAIQGGIPAGQYTSVVRADPVRRGLLYA
                     GTVGGVFVSFDDGDHWQSLQMNLPPAWVGDLQVHGADLVAATNGRALWVLDDVTPLR"
BASE COUNT         1072 a         2090 c         2150 g         1004 t
ORIGIN      
        1 cggtgaagaa gatcgtccct tccgggtcga aggcaaggct gtgcgggtcg cgggcacgcg
       61 agtcgggcat cgcgtactcg gtgacctttc cggtgagcgg atcgagcttc ccgatgtacg
      121 ccgcggagtt ggccgtgaac cagatgttgc cgtcctttcc cgccgcgaga ccgtgcggtc
      181 cggaatccgc cttcggcaga gcgaactcct tgatcgtccc ggtcctcgga tcgagccggc
      241 ccattttgtt cgcgagctgc ccggtgtacc agagcgatcc gtcgggcgcg acctccggat
      301 cgtgcgggcg agagctcgcg gtcgggacgt cgaattcccg gatcgagacc ttccactcag
      361 ccgctccaag cgcgggcgga agcagaagga gaaagagacc gaccggggcg atccgccggc
      421 gcatcggcgg ccttagacct cgaccacggc cgcgtgctcg acgcgggtgc caagcacatg
      481 gaggaactga gcgagccaca ccggatgcgc gggccacgcc ggcgccgtaa cgagatttcg
      541 gtccgtcact gcctggtcga ccgggatgtc cgcataagtc ccgcccgcgc gcgtgacctc
      601 gggtcccacc gccggatagg aggagcagcg cctcccgctg acgacgccgg ccgccgcgag
      661 gatctgcgct ccgtggcaga tcgaggcgat cggcttctcg cgcgcggcga agtgcttcac
      721 gatttcgagc acgcgtgggt tcaggcggag atactcgggg gcgcgcccgc ccgggatcac
      781 gagcgcgtcg tagtcgcctt cccgaaccgc gtcgaagtcg aaattcaacg agaagttgtg
      841 gccccgcttc tcgctgtagg tctggtcccc ctcgaagtcg tggaccgccg tgcggacttt
      901 ctcgcccgcc ttcttgccgg gacagacggc atgaacggtg tgtccgacca tgagcaacgc
      961 ctgaaacgga accatgacct cgtagtcctc gacgaaatct cccaccagca tcaggatctt
     1021 cttgcccgcc atcagaacct ccttggaaga atgcgattct attgggaaca ttcttcgtgc
     1081 gggagcgtac cgggttgaca tgagctccac aacgctgcga tggctgggta ccctgaccgc
     1141 tctcgtgctt gtccctgtcg ctgcccgggc cgaggtgacg cgaacgctgc gcgcgcaaat
     1201 cccgggcgcc gcgtcccgcc ccgtggcggt cgagaacctt ctcgggacga cgcgggtgac
     1261 cgtcggcacg gcggacgcgc tcgtggtcgt cgccacggtc cacgccgaaa acgacgcgct
     1321 cgcttcgtcg gtccgcctgg acgagcttcg cggcgatggc ggcgtcgcga cgctgcgcat
     1381 ccgttacccg gcaatcagtc gcgttctccg ttatccccgg cgcgatcacg agcaccgctg
     1441 ggacggactc gttctctttg gaaacggaaa tgtcgagcga tacgacggag cgcgataccg
     1501 cgtgagctcg caccgcggca cgctcctcta cgccgacgtg gaggtgcagg tgcctgcccg
     1561 cgttgcggac gcaacttttc tcgaaacggt cggggagctc gacgccgacg gcctggaggg
     1621 aaagctctcg ttccgggtcg ccagcgccga cctgcgactc gaccgcctga atggagacgt
     1681 cgaagtcacg ggcacgtctg gcgacacccg cgcgtcccgg atccggggaa cctggcgctc
     1741 tgatttctcg agcggcgaca gcgagatgga gcggttcgac gggtcgtcgg cgacgttcca
     1801 cacgagctcc ggcgatatcg cggtgcgtgg gatcgcgtcc gagcgcctgt cgatcacgac
     1861 gagctccggc gacgcccgcg tccgcgatgc ggacgtgcgg gagctccaga ccgatgcttc
     1921 ctccggcgac ctctcgttcg aaaccgcggg cactcggctg gcacgtgttc tcgtgcgctc
     1981 ttcgagcggc gacgttcacc tcgcgctcgc gcccgaggcg ccgttccagg ccgaagccga
     2041 tcaatcgagc ggcgagatgc gcgttcgttt cgacgacggc gaacgacgct accggcgcgg
     2101 cgatctggtt gcgttccgcc gcggcacggc aggtgccgac atccgggtga cgacctccag
     2161 cggagacttc acgatcgatc cacgataggt cccgtcccgc cgtagaatcg cctccccgtt
     2221 ttcgggccta tgtcgttcag gtctttcgga ggaggaaaaa tggctgattc gaaccgaacc
     2281 gttctcgtca ccggcgccac cggaaaccag gggggctccg tcgctcagga actcctgtcc
     2341 cgcgggggct accgagtccg cgcgatgacg cgaaagcccg acggcgaaaa ggcgcgagcc
     2401 ttgaaggatc gcggcgcgga aatcgtcgca ggagacctga acgacgaggc atcgctgcgc
     2461 aaagcgatgc agggagcctg gggtgtcttc gccgtgcaga acacgtggga ggcgggggtc
     2521 gaaggcgagg aagagcaggg caagcggcag gcgaaggtcg cccgcgacgc cggcgtgcag
     2581 cattacgttt acacgtcggt tgcgtccgcg gaccgcaaga cggggatccc gcacttcgaa
     2641 aacaagtgga ggatcgagga aactgtccgc gggctcgggt tcccgtcgca cgtgattctg
     2701 cgccccgtgt tcttcatgga gaatcttctc gggccgtggt tcaagtcggc catcgaccag
     2761 gggcagctcg cgatggctgt caagccggaa acgcgactcc agatgatcgc ggtcgaggac
     2821 atcggcaagt acgggcggtg ggcgttcgac aaacaagaga cgttgaatgg tcgcgagatc
     2881 gacatcgcgg gagacgaact gacgatgccc gagaccgcgc gcttgttgag ccgggcgtcc
     2941 ggacacccgg tcgcgttcgc gccgccgccg atcgaggccg tgcgccaggg gagcgcggat
     3001 ttcgcggcaa tgctcgagtg gttcgaccgc gtcggctacg atgccgacat cgcggcaaac
     3061 gcgcgcgagt cggggatccg cccgacgacg ttcgccgagt gggcgtcccg gcagaagtgg
     3121 tcgtagggcg accgcgtccc tcgaggcgtc cgtagcggga gcgagaagaa ggacgtactc
     3181 gaccttccct tcgccatctc cggatgggaa gaccggcaat gtggcaagtg gagacctgac
     3241 cccgtatgct ccctcactct tgatgctctc accattgctg ctggcggtct cgctgatcgc
     3301 gaccgcccgg ggattccagg cgccggagag cgtcttctgg gacgaggtct cccggacctg
     3361 gttcgtctcg aacgtcgtcg ggaaggagaa cgaaaaggac ggcaagggct ggatcagccg
     3421 gctcgacgcg aacgggaagg tcgtccaacg gaaatgggtc gagggtctcg actctccgca
     3481 cggcgtccgc gttcacgccg gaacgctcta cgccgccgac gtcgaccgga tcgtcgtgat
     3541 cgacgtggcg aaggcacgaa ttcgcgagcg gatatccgcg cccggcgcga aattcctgaa
     3601 cgacgtcgcg gtcgggccgt ccggagacgt ctacgtttcg gacacgctcg gcgacgcgat
     3661 ctaccgctgc acgacgtcct gcgaggtctt ggtgaagtcc gacgctctcg aaggccccaa
     3721 cggcctgctc gtcgacggtg accgcttgat cgtggccacg tggggcccga tcacggaccc
     3781 gatcaccttc gcgacgaaga cgcccggtcg cctgatttcg gtcgacctga agacgaaaca
     3841 gatcgcgccg ctcggcgagg gaaagccgat cggcaacctc gacggtatcg agaaggacgg
     3901 cgccgactac ctcgttacgg acttcatggc cggcaaaatc tttcgcgtgt cgaagtcggt
     3961 cgcggccacc gttctcaagt ccggcttcgc ggagagcgcg gacctcggct gggacccggg
     4021 ccgccggatc atcgcggtgc cggaaatggg tgggggaaca gtgaagttct tccgcctttc
     4081 ccgataggag ctcttcggat gaaaaagctg ttggtggccg gaatgctcgc ggcgtgctcg
     4141 ctccttcccg ccgccgcgca gacgatcgac ccttccctgt tcgccgaaat gcgctggcgc
     4201 ctcgtgggac ctttccgcgg cggctgggca acgatggccg agggaatccc ggaccagccc
     4261 gacaccttct tcttcggagc ggcgggcggc ggcgtctgga agaccgacga cgcgggacgg
     4321 acgtggacgc ctctgttcga caaagcgtcg accgcgtcga tcggcgccct cgcgatcgcg
     4381 ccgacggacc cgaaggtgat ctacgtcggc acgggacagc cgcagccgcg ctacgacgtc
     4441 atctccggcg acggcgtgtt ccgctcggac gacggcggaa agacgtgggc gcactgcggc
     4501 ctcgaagcga cgcgccacat cggccgcatc ctcgtcgatc cgcgagacgc gaacacggtg
     4561 ctcgtcggcg cgctcggaca catcttcggg cccaaccgtg agcgcggcgt cttccgttcg
     4621 accgacggcg gccggacgtg gagcccggtg ctcttcgtcg acgagagcac gggcgtcgtc
     4681 gatctcgcga gcgacccggc gcagcccgat gtcgtttacg cctcggcgtg gcagttccga
     4741 aactatccgt ggctctcgta cttcaagccg aacaccggac cgggtagcgg cgtctaccgg
     4801 tccaccgacg gcgggcgcac gtggaaacgc ctctccggga gcgggtggcc gacagaaaac
     4861 ctcggcagga ttggcctctc cgtctcgccg ggcggccgcg tgtgggcgct cgtcgacgcg
     4921 cagtcggcgc tcgcgtggct cgatccgacg cgggcgacga cgtcacccgc cggcctttat
     4981 cgttccgacg acggcggcgc gacgtggagc cacgtcaaca ccactcccgg ccttgcgagc
     5041 tactacatgg gtcgcgtcac ggccgatccg aaaaacagcg acgtcgtcta cgtcatgggt
     5101 cagtccatcc ggcgctcgga cgacgggggg aagacgtttc gcatcgtgaa gggcgcgccg
     5161 ggcggcgacg actaccactt cctctggatc aacccgaagc atcccgacca catggtcacc
     5221 tctgcagacc aggggacggt cgtgaccgtg aacggcggga ggacctggag cgactggtac
     5281 aaccagccga ccgggcagtt ctaccacgtc gagaccgacg atcgtttccc gtactggatc
     5341 tacagcggcc agcaggacag cggcaccgcc ggcacgatga gccgcagcga cttcggcggc
     5401 ctgacgttcc gcgactggca tcccgtcggc gccgaggagc gcgggtggga cgtccccgac
     5461 cccgccgatc ccgacatcgt ctatggcacg ggtctcggcg gcaacgtgat gcggttcgac
     5521 cgccgaacga agcaggccgt caacatctcg ccggtggtcg agagcacgta cgcgaggcgc
     5581 ccgacggagg tgaagtaccg ctacacgtgg atcacgccgc tcgcggtgtc gaagaaggcg
     5641 ccgcacgcga tctactacgg ctcacaggtg ctgtttcgct cgaccgaccg cggccagagc
     5701 tggacggtcg tgagcccgga cctgacgggc gcgaagccgg ggaccgccgg ctgcgacggc
     5761 gagatcacgc tcgcgaacgc ttcgccgtgc gggttcggag tcatcttcac gatcgccctc
     5821 tcgccgagtg acgagaacga gatctgggtc gggacggacg acggccgcat ccaggcgacg
     5881 cgcgacggcg ggaagagctg gaacgacgtc acaccgaaga atctccccgc gtggagcaag
     5941 gtttcgtcga tcgacgtgtc ggcgctcgag accggcacgg cgtatgccgc gatcgacacg
     6001 caccgcctgg acgacttctc gccgcacctc ctgcgtacgc acgacggcgg gaagacgtgg
     6061 accgcaatcc agggcggaat tcccgcgggc cagtacacga gcgtcgtgcg cgccgacccc
     6121 gtgcgccggg gactcctcta cgccgggacg gtcggcggcg tcttcgtttc attcgacgac
     6181 ggcgatcact ggcagtctct ccagatgaac ctccctccgg cgtgggtcgg cgacctgcag
     6241 gtccacggcg ccgacctcgt cgccgcgacg aacgggcgcg cgctgtgggt cctcgatgac
     6301 gtgacgccgc tccgcc
//
LOCUS       DASWAV010000476         6003 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U2665
            k141_427660, whole genome shotgun sequence.
ACCESSION   DASWAV010000476 DASWAV010000000
VERSION     DASWAV010000476.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960316
            Sequence Read Archive: SRR10260007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 6003)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6003)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:47:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,929
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6003
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_427660"
                     /isolate="SMAG_U2665"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.36444 N 132.31889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..891
                     /locus_tag="VF958_09875"
     CDS             <1..891
                     /locus_tag="VF958_09875"
                     /inference="COORDINATES: protein motif:HMM:NF014344.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine deiminase family protein"
                     /protein_id="HEX9149456.1"
                     /translation="YWLPPIPNYFFQRDPLVVVGDRAIRGSMATSARLREPLLSGYVF
                     RYHPRFGDPGLDRFWFQEFSADYGRPSSYARMRPTLEGGDILVLREDLLLMGYSERTK
                     KTTIERLAESLREKNSPVRRILVVAVPPARSFMHLDTVFTMISNEECLIYAPMILPDG
                     AEEADVYRCDLTSRPAAWTSETDLLSALKAEGLDLAPVRCGGADPISERREQWTDGAN
                     AFALAPGVVLIYDRNERTAEELANAGYEVVDEADLLLGKNELDLRRRKKYAVRLAAHE
                     LSRARGGPRCMAMPLVREET"
     gene            888..1859
                     /locus_tag="VF958_09880"
     CDS             888..1859
                     /locus_tag="VF958_09880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011994912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HEX9149457.1"
                     /translation="MSTLFFPFVRPLAVVAFGGNALLRPEDHGDTESQLARARTAVRQ
                     LLPVLNRGYELIVVHGNGPQVGNLLLQGEIAAERIPPPSLDVCVAQTQGSMGFLLELA
                     LDNELRAAGFRKRVVTLVTEVEVDAADPAFLEPSKPVGPFFSRERAEAIQRTAGWTMV
                     EDAGRGFRKVVASPKPRAIRNVDVVAVLVNRGSIVVACGGGGIPVVVPEEGEVRGVEA
                     VIDKDYAASMLAASLSADLFVILTGVERVSKDFGKASETPLARLDVATARALLAEGQF
                     PPGSMGPKIDAAIRFVEAGGREVLITRAECLSAALDGETGTVIWRTE"
     gene            1856..2776
                     /gene="argF"
                     /locus_tag="VF958_09885"
     CDS             1856..2776
                     /gene="argF"
                     /locus_tag="VF958_09885"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010349642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004585 - ornithine carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HEX9149458.1"
                     /translation="MKRDYVNENDWAPAEIEAVLDLAARIKGRPGDWKTALEGKCLAM
                     IFEKSSTRTRVSFEAGMFQLGGLGMFLSSRDLQLGRGEPISDTARVLSRYVDGIMART
                     YAHSTIEDLARFGSVPVINGLSDLLHPCQALADYLTVRERFGTSRGFPLCYVGDGNNV
                     AHSLLLTGAKLGARVSVVTPPGFAPDPRIVEWSRAAARETGGSVRITTSLEEGLAGAR
                     AVYTDVWASMGQESEAQDRAKKFEPYRVTRSVMAMAEPDAIFLHCLPAHRGEEVEADV
                     IDSPAAMVFDQAENRLHAQKALLVTLLGSG"
     gene            2776..3819
                     /locus_tag="VF958_09890"
     CDS             2776..3819
                     /locus_tag="VF958_09890"
                     /inference="COORDINATES: protein motif:HMM:NF015506.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="HEX9149459.1"
                     /translation="MPIGQPPPGARAAVFGAWRRGFWIATGTLALVAVMLVVGRGRRG
                     VRSAPPESPAAVTVPEAVLHREPSASAPAISALKRGAEVTVLSDRGAWLEVKAGRETG
                     FVRAGEIEREEEREAREERAKRILSFPPVYGVVAETTAVLLAPFPFAPRAGTLSKGTV
                     IPIHAVDHAYFAYRDSANGVAFVASADVDLVPPDPKSPNIAPDSSIALRRLSTFDVPT
                     PIPTEVEAPGLPPQAEGGAPEVPPAGRPPSSGAPQGSSIEPAVVVEKVDPPYPEVAVR
                     ARIEGSVVLDIGIDETGRVTDVQVTRGLPFGLSEAAADAVRRWKYRPARGPEGPVPSR
                     KTVRILFTLRAAG"
     gene            3865..5541
                     /locus_tag="VF958_09895"
     CDS             3865..5541
                     /locus_tag="VF958_09895"
                     /inference="COORDINATES: protein motif:HMM:NF012437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HEX9149460.1"
                     /translation="MLQVMFEADGRAQVYTLAREQASIGRSSDNDIVLNDFSVSRRHA
                     YLRHENGEWMLYDNHSTNGIRVNDRHVPNAPVGDGDRAVIGTFTLRFREQAEEPEEES
                     SPIKMDSTSTCIRPIAEFNLDFGLEKSDTPRPAIESTADRKRVVLDLAYKNKIFEILV
                     QVAKTLISLEDLETVLEKVIDLIFEYLPVDRGFLLLEENGALRLRISRFKGGQRMTTD
                     GSLPYSRTIVDMVVRDKVAVLTSDAQRDERFEAGQSVRIQQIRSAMCAPLWNRDSVIG
                     VIHVDSPLHVGSFTERDLDLLTALANFAAVAIERARLHDRVAEEKRIRGRLERYHSPQ
                     VVDEIIADVQATGSFKEVRTKTVTILFADLVGFTTWSEKMAPSELARLLNRFFTLASD
                     AVFSQDGTIDKFVGDAVMAFFGAPIDQPDHAVRAAVAALKVREEVAEWNKERASDGMF
                     PLEVRIALNTGETIVGEIGSERRVDYTVLGNPVNVAARMEEFVAMPGDIVIGPATYEA
                     TRDRFEVAQLGFFALKGVSAQIPLYKLLGAREAPQLELAQGPKSQVQGPK"
     gene            5555..>6003
                     /locus_tag="VF958_09900"
     CDS             5555..>6003
                     /locus_tag="VF958_09900"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013854.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0047429 - nucleoside triphosphate
                     diphosphatase activity [Evidence IEA];
                     GO_process: GO:0009146 - purine nucleoside triphosphate
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="non-canonical purine NTP pyrophosphatase"
                     /protein_id="HEX9149461.1"
                     /translation="MILAGYAFVTGRPEKAQEALRMGFALERIDLDLPEQQSVDSSEV
                     ALIKVRAAFDRLGRPVIVEDSGLEVLAWGGFPGALVKWLEKTAGLEAITRMLDPFPDR
                     RARAVCAIACFDGKTIIAARGVADGWIAPAPRGSGGFGWDPIFVPEGS"
BASE COUNT          949 a         1995 c         2057 g         1002 t
ORIGIN      
        1 tactggctgc cgccgattcc gaattacttc ttccagcgcg acccgctcgt ggtcgtgggc
       61 gaccgggcga tccgcggttc gatggcgacc tccgcgcggc tccgcgagcc gctcctttcc
      121 ggttatgtct tccgatacca cccgcgcttc ggagatcccg ggctcgaccg cttctggttc
      181 caggagttct cggccgacta cggccggccc tcgtcctacg cccgaatgcg gccgacgctc
      241 gaggggggag acattctcgt cctccgcgaa gaccttcttc tcatggggta ctcggaacgc
      301 acgaagaaga cgactatcga gcgtctcgcc gaatcgcttc gcgagaagaa ttccccggtg
      361 cgacggatcc tcgtcgtcgc ggtgccgccc gcgcgctcgt tcatgcacct cgacaccgtc
      421 ttcacgatga tctcgaacga ggagtgcctg atttacgcgc cgatgatcct tcccgacgga
      481 gccgaggagg cggacgtcta ccggtgcgac ctgacgagcc ggccggcggc gtggacctcg
      541 gaaacggacc ttctttcggc gttgaaggcc gagggcctcg acctggcgcc ggtccgctgc
      601 ggcggcgcgg accccatctc ggagaggcgc gagcagtgga ccgatggagc caacgcgttc
      661 gctctcgcgc ccggagtggt gctgatctac gaccgcaacg agcgcaccgc cgaagagctc
      721 gcgaacgcgg gatacgaagt cgttgacgag gcggatctcc ttctgggaaa gaacgagctc
      781 gatctgcgac ggcggaagaa gtacgcggtt cgactcgccg cgcacgagct ttcgcgggcg
      841 cgcggcgggc cgaggtgcat ggcgatgccg ctcgtgcggg aagagacgtg agcacgctct
      901 tcttcccctt cgtccgcccg ctcgccgtcg tcgccttcgg cggaaacgcc ctcttgaggc
      961 ccgaagacca tggcgatacc gagtcccagc tcgcccgcgc ccgcacggcg gtgcggcagc
     1021 tcctcccggt gctgaaccgc ggctacgagc tcatcgtcgt ccacggcaac gggccgcagg
     1081 tcggcaacct gctgctccag ggggagatcg cggccgagcg gattccgcct ccgtccctcg
     1141 acgtgtgcgt ggcgcagacc cagggctcga tgggtttcct gctggagctc gcgctcgaca
     1201 acgagctccg cgcggccggg ttccgcaagc gcgtcgtcac cctcgtcacg gaggtcgagg
     1261 tggacgccgc ggacccggct ttcctcgaac cttcgaagcc cgtcggtcct ttcttttcac
     1321 gggagagggc cgaggccatc cagcgaacgg cgggatggac gatggtcgaa gacgcgggcc
     1381 gcggtttccg gaaggtcgtt gcctcaccga agccgcgggc gatccgcaac gtcgacgtcg
     1441 tcgcggtgct cgtcaaccgc ggctcgatcg tcgtcgcgtg cggcggcggg gggattccgg
     1501 tggtcgtgcc cgaggagggg gaagtgaggg gcgtcgaagc ggtgatcgac aaggactatg
     1561 cagcctcgat gctcgcggcg tcgctctccg ccgacctctt cgtgatcctg accggggtcg
     1621 aacgcgtttc gaaggacttc ggaaaggcga gcgagacccc gctcgcgagg ctcgacgtgg
     1681 cgaccgcccg cgcgctgctc gccgagggac agtttccccc gggcagcatg ggtcccaaga
     1741 tcgacgcggc aatccggttc gtcgaagcgg gcgggcggga ggtgctgatc acgcgagcgg
     1801 agtgcctctc ggccgcgctc gacggcgaga ccggaaccgt gatctggagg acggaatgaa
     1861 gcgcgattac gtcaacgaga acgactgggc gccggccgag atcgaagccg tcctcgacct
     1921 cgccgcgcga atcaagggcc gcccgggcga ctggaagact gcgctcgagg ggaaatgtct
     1981 cgcgatgatt ttcgagaagt cgtcgacccg gacgcgcgtc tcgttcgaag ccggcatgtt
     2041 ccagctcggc gggctcggaa tgttcctgtc ctcgcgcgac ctccagctcg gccggggcga
     2101 accgatctcg gacaccgcac gggtgctctc ccggtacgtc gacggcatca tggcccgcac
     2161 gtacgcgcac tccacgatcg aggatctcgc gcgcttcggg tcggtgccgg tcatcaacgg
     2221 gctgtccgac ctcctccacc catgccaggc gctcgccgac tacctgacgg tccgcgagcg
     2281 cttcgggacc tcgcggggct ttccgctctg ctacgtgggg gatggaaaca acgtggccca
     2341 cagccttcta ttgacgggag ccaagctggg cgcacgagtg tcagtcgtca cgccgcccgg
     2401 cttcgcgccc gacccgcgga tcgtcgagtg gtcgcgcgcg gcggcgcgcg agaccggggg
     2461 atccgttcgg atcacgacct ctctcgaaga ggggctggcc ggcgcgcgcg ccgtgtacac
     2521 ggacgtctgg gcttcgatgg ggcaggagtc ggaagcgcag gaccgcgcga agaagttcga
     2581 gccctatcgc gtcactcgga gcgtgatggc gatggcggag cccgacgcga tcttcctcca
     2641 ctgccttccc gctcaccggg gggaagaggt cgaggccgac gtgatcgatt cgccggcggc
     2701 gatggtcttc gatcaggccg agaaccggct tcacgcccag aaggcactgc tcgtgacgct
     2761 cctgggaagc gggtagtgcc gatcgggcag ccgccgcccg gcgctcgcgc cgcggtgttc
     2821 ggcgcgtggc gccgcggctt ctggatcgcg accgggacgc tcgcgctcgt cgccgtcatg
     2881 ctggtcgtcg gacgcggtcg gaggggcgta cgcagcgctc ctccggaatc tcctgcggcc
     2941 gtcacggtcc ccgaggccgt cctgcatcgg gagccgtcgg cgagcgcgcc ggcgatcagc
     3001 gcgttgaaac gcggcgccga ggtgacggtc ctttccgatc ggggagcctg gctcgaagtg
     3061 aaggcaggaa gggagacggg attcgttcgc gccggcgaaa tcgagcgcga agaggagcgc
     3121 gaggcccgcg aggagcgagc gaagaggatc ctctcgtttc cgccggtcta cggcgtggtc
     3181 gcggagacga cggccgtcct cctcgcgccg tttcccttcg cgccgcgagc gggcactctt
     3241 tcgaaaggga ccgtcatccc gattcacgcc gtcgaccatg cgtactttgc ctaccgcgac
     3301 agcgcgaacg gggtcgcttt cgtcgcctca gcggacgtgg acctggtgcc tccggatccg
     3361 aagagcccga acatagcccc cgactcgtcg atcgcgctgc ggcggctctc cacattcgat
     3421 gtgccgacgc cgattccgac cgaggtcgaa gctccggggc tgcctccgca ggcggagggc
     3481 ggcgccccgg aggtcccgcc ggccgggcgc cctccatctt ccggcgctcc ccagggcagc
     3541 tcgatcgagc ccgccgtcgt cgtcgaaaag gtggatcctc cgtatccgga agtcgccgtc
     3601 cgcgcccgga tcgagggaag cgtcgtcctc gacatcggga tcgacgaaac tggtcgagtg
     3661 accgacgttc aggtgacgcg cgggctgcct ttcggcctct ccgaggcggc ggcagacgcc
     3721 gtgcggagat ggaagtaccg tccggcgagg ggaccggaag gtccggttcc ctcacgcaag
     3781 accgtccgca ttctcttcac gctgcgggcc gccgggtagc agtcgcgctc caggtcccgg
     3841 cttcgaccta taatttttct ttcaatgctc caggtcatgt tcgaggcgga tggtcgcgcg
     3901 caggtctaca cgctcgcgcg cgagcaggct tcgatcgggc ggtcgagcga caacgacatc
     3961 gtcctgaacg acttctcggt ctcccggcga cacgcctacc tgcgtcacga gaacggcgaa
     4021 tggatgctct acgacaacca ttcgacgaac gggatccgcg tcaacgatcg ccacgtcccg
     4081 aacgcgccgg tgggagacgg cgatcgtgcg gtcatcggca ccttcacgct tcgtttccgc
     4141 gaacaggcgg aggagccgga ggaggagtcc tcgccgatca agatggactc gacgtcgacc
     4201 tgcatccggc cgatcgccga gttcaacctc gacttcggtc tcgagaagag cgacacgcct
     4261 cggccggcta tcgaatcgac ggcggaccgg aagcgcgtcg ttctggacct cgcctacaag
     4321 aacaagatct tcgagattct cgtccaggtc gcgaagaccc tcatctccct cgaggacctc
     4381 gagacggtcc tcgagaaagt catcgatctc attttcgaat acctcccggt cgaccgcggg
     4441 tttctcctcc tggaggagaa cggagctctg cggcttcgca tcagccgttt caaggggggc
     4501 cagcgcatga cgacggacgg ctcgctcccg tactcgcgga cgatcgtcga catggtcgtc
     4561 cgcgacaagg tcgcggtcct gacgtccgat gcccagcgcg acgagcgctt cgaagcgggg
     4621 cagtcggtcc ggatccagca gatccgttcc gcgatgtgcg cgccgctctg gaaccgggac
     4681 tcggtcatcg gagtcattca cgtcgattcg ccgctgcacg tcggatcctt caccgagcgg
     4741 gacctcgatc tcctgaccgc gctcgccaat ttcgcggccg ttgccatcga gcgcgcgcgt
     4801 ctgcatgatc gcgtcgccga agagaagcga atccgggggc gactcgagcg ctaccactca
     4861 ccgcaggtgg tcgacgagat catcgcggac gtccaggcga ccggctcgtt caaggaggtt
     4921 cgcaccaaga cggtcacgat cctcttcgcg gacctcgtcg ggtttacgac ctggtcggag
     4981 aagatggcgc cgagcgagct cgcgcggctt ctcaaccgct tcttcacgct cgcatccgac
     5041 gcggtctttt cgcaggacgg cacgatcgac aagttcgtcg gcgacgctgt gatggccttc
     5101 ttcggcgcgc cgatcgatca gccggatcac gccgtgcgcg cggcagtcgc ggcgctgaaa
     5161 gtccgcgaag aggtggcgga atggaacaaa gagcgcgcgt cggacggcat gttcccgctc
     5221 gaggtccgga ttgcgttgaa caccggcgag acgatcgtcg gagagatcgg ttcggagcgc
     5281 cgagtcgact acacggttct cggaaatccg gtgaacgtgg ccgcccggat ggaggagttc
     5341 gtggccatgc ccggcgacat cgtgatcggg ccggcgacat acgaggcgac acgggatcgg
     5401 ttcgaggtcg cgcagctcgg cttcttcgcc ctgaagggcg tctcggcgca gattccgctt
     5461 tacaagctgc tcggcgcacg cgaggcgccg cagctcgagc tcgcccaagg tccgaagtcc
     5521 caggtccagg gtccgaaata gcccgaatcc gcgcgtgatc cttgccgggt acgcgttcgt
     5581 caccggccgt cccgagaagg cgcaggaggc cctgcgcatg ggcttcgcgc tcgagcggat
     5641 cgacctcgac cttccggagc agcagtcggt cgattcctcg gaggttgcgc tgatcaaggt
     5701 ccgggccgcg ttcgaccgat tgggccgccc ggtgatcgtc gaggactcgg gcctcgaggt
     5761 cctcgcgtgg gggggttttc ccggagcgct cgtcaagtgg ctggagaaga ccgccggtct
     5821 cgaggcgatc acacgaatgc tggacccgtt tccggaccgg agggcccgcg cggtgtgcgc
     5881 gatcgcatgc ttcgacggca agaccatcat cgcggctcgc ggagtcgcgg acgggtggat
     5941 cgctccggcc ccgcgcggct ccggggggtt cgggtgggac ccgatcttcg tcccggaggg
     6001 atc
//
LOCUS       DASWAV010000477         4701 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U2665
            k141_65342, whole genome shotgun sequence.
ACCESSION   DASWAV010000477 DASWAV010000000
VERSION     DASWAV010000477.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960316
            Sequence Read Archive: SRR10260007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 4701)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4701)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:47:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,929
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4701
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_65342"
                     /isolate="SMAG_U2665"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.36444 N 132.31889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1091)
                     /gene="yidC"
                     /locus_tag="VF958_09905"
     CDS             complement(<1..1091)
                     /gene="yidC"
                     /locus_tag="VF958_09905"
                     /inference="COORDINATES: protein motif:HMM:TIGR03593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0051205 - protein insertion into membrane
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HEX9149462.1"
                     /translation="MEKRLLVAAFLSLGVLLLWEWLGPKAPRRIGPVAAPTPSATVLP
                     TAAAPATAAALAPTATAETVEPIAGTQEGLTTLENAVARATFSNRGAVLTSFVLLKHF
                     DDQKRPLELVRALPDPAMRPLAVSFNGNPDLTRAAANALYAVEPGTGSARRTLTFRYA
                     DARLSITKEIRFGDGYLFDVKVSAGGPACTVVAATGLRNPTGQERESRYVMPASAFAT
                     SGSGIEVFSPAKLKKEESRALAPNGFVGIEDNYFLAALITRQAATARVLPETLPPAPG
                     APAASKGDPTVSAGVSSAGALDLRAFFGPKDVEVLEQTGLGLERTVNFGWYGIVARPL
                     LWLLKKAYSWTGNYGIAILAVTLLIRILL"
     gene            complement(1099..1317)
                     /gene="yidD"
                     /locus_tag="VF958_09910"
     CDS             complement(1099..1317)
                     /gene="yidD"
                     /locus_tag="VF958_09910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006696491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HEX9149463.1"
                     /translation="MSFGARAATLVLRFYKTAISPILPRACRYEPTCSVYAREAIERY
                     GLWRGAGLAIARLARCHPFRPGGFDPVP"
     gene            complement(1314..1736)
                     /gene="rnpA"
                     /locus_tag="VF958_09915"
     CDS             complement(1314..1736)
                     /gene="rnpA"
                     /locus_tag="VF958_09915"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: protein motif:HMM:NF013022.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004526 - ribonuclease P activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HEX9149464.1"
                     /translation="MSSGPDRRRETFSRDDRLRKRQEFEECYASGVRVSGRHLQVFLL
                     TAGTARPSPDPESAGRPRLGLSVSRRVGGAVARNRVRRRLREIFRRNRDVFGGRGGNL
                     VVNAKPSAAKASFTELAEEYRSLVARTLSRAPERQVPR"
     gene            complement(1733..1867)
                     /gene="rpmH"
                     /locus_tag="VF958_09920"
     CDS             complement(1733..1867)
                     /gene="rpmH"
                     /locus_tag="VF958_09920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011918258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HEX9149465.1"
                     /translation="MKRTYQPNNRRRRRTHGFLVRMRTRSGRAVISARRRKGRKKLSV
                     "
     gene            2092..3402
                     /gene="dnaA"
                     /locus_tag="VF958_09925"
     CDS             2092..3402
                     /gene="dnaA"
                     /locus_tag="VF958_09925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019004990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HEX9149466.1"
                     /translation="MSEIWSSIVKNLEGKLDPKELKTWFAPTRQVSFDRRGEVGALTV
                     AVPSPVFAEWIGARHSALLAREAAAVGFPGLDIQFLPIAVETPIPPAPVLPHPRRLVL
                     NPRFTFDTFVVGSSNQFAHAAARAVGESPSRSYNPLFLYGGVGLGKTHLMHAIAHEIL
                     QRSPESRVAYLSAERFLNELINALRFEKMHEFKSRYRELDVLLMDDIQFIAGKDSTQE
                     EFFHTFNALHDAQKQIVVTSDALPKEIPTLEERLRSRFEWGLIADIQPPDLEMKVAII
                     RKKAAAEKAEIPNDVALFIAGTVKSNIRELEGRLNRVLAFSSLTGKPVSVELARETLR
                     EILGHEENRAIPSEIVKTVASHYGLRVSDLKAKTNAKPIAFPRQVAMYLCRRLTGLSY
                     PEIGRLFNDKHHSTVMHSVEKIEKMVEDDPDFRKVIEGFQQPYR"
     gene            3632..>4701
                     /gene="dnaN"
                     /locus_tag="VF958_09930"
     CDS             3632..>4701
                     /gene="dnaN"
                     /locus_tag="VF958_09930"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HEX9149467.1"
                     /translation="MELTLKRGDFLNELALMQGVVERKNTIPILSNILLKATDGHLDL
                     TSTDLDLTLLSQAEAKVSRPGSVTVPARKLFDLIRNLPEADVRLKLMENHYLGVTCER
                     SNFRLVAQPAEDYPTVPKVEGKAGMTFPLEAWKRLTRKVLYAVSAEETRFQLMGALLK
                     DKGKEFELVATDGHRLALVNFGKENVRGTLPNVLIPKKALAEILKMDGGESGGELEIK
                     HSENHLLFRVGNRQLIARLLDVNFPDYEKILGKGNDKVAIVPRVACQSAIRRVALFSP
                     ERARGVRLSLEKDLLTITSASQELGDGSETLDVRYDSEPLSIGLNAQYLLDFFDEVET
                     ENVRLELKDENTQCLCHPEGEV"
BASE COUNT          848 a         1526 c         1527 g          800 t
ORIGIN      
        1 aagaggagga tccgaatcag cagggtcacg gccaggatcg cgatgccgta gttcccggtc
       61 cacgagtacg ccttcttgag tagccacagg agcggccgcg cgacgatccc gtaccacccg
      121 aagttgaccg tgcgctcgag gccgaggccg gtctgctcga ggacctcgac gtccttcggc
      181 ccgaagaacg cgcgcaggtc gagcgccccc gctgatgaaa ctcccgcgga gacggtcggg
      241 tcccctttcg acgccgccgg ggcgccgggc gcgggcggga gggtctcggg caggacgcga
      301 gcggtcgcag cctgccgcgt gatgagggcc gcgaggaaat agttgtcttc gattccgacg
      361 aacccgttcg gggccagcgc ccggctctcc tctttcttca gcttggcggg gctgaagact
      421 tcgatcccgg agcccgaggt cgcaaacgcg ctcgccggca tcacgtaacg ggactcgcgc
      481 tcctgtccgg tcggattgcg aagtccggtc gcggcgacga cggtgcaggc ggggccgccg
      541 gccgaaacct tgacgtcgaa caggtagccg tcgccgaatc ggatctcctt ggtaatcgaa
      601 agacgcgcgt ccgcgtagcg gaaggtgagc gtccggcggg cggagccggt tcccggctcg
      661 acggcataca gcgcgttcgc tgcggcgcgc gtcaggtcgg gattgccgtt gaacgagacg
      721 gcgagaggcc tcatcgcggg atccggcaac gcgcggacca gttcgagggg ccgcttctga
      781 tcgtcgaagt gtttgaggag gacgaaggac gtcagaacgg caccgcggtt ggaaaacgtg
      841 gcgcgcgcga ccgcgttctc gagcgtcgtc aacccttcct gtgtacccgc gatcggctcg
      901 actgtctcgg ccgtcgccgt tggcgcgagc gcggctgcgg tcgctggagc ggccgccgtc
      961 gggagaaccg tcgcgctcgg cgtcggtgcc gcgacgggac cgatgcgccg tggcgccttc
     1021 gggccgagcc actcccagag cagaagcact ccgagggaga ggaatgcggc gacgaggaga
     1081 cgcttttcca tcttcgcgtc agggaaccgg gtcgaatccg ccgggacgga aagggtggca
     1141 tcgagcgagg cgggcgatcg cgagtcccgc gccgcgccag agcccgtaac gctcgatcgc
     1201 ttcgcgcgcg tagaccgagc aggtcggctc gtaccggcag gcgcgcggga gaatgggcga
     1261 gatcgccgtc ttgtagaagc gcaggacgag agtggccgct cgggcgccga agctcaacgc
     1321 gggacctgcc tctccggcgc tctcgacagg gtgcgggcga cgagcgaccg gtactcctcc
     1381 gcgagctccg tgaacgaggc cttcgccgcc gaaggtttcg cgttgacgac gaggtttccg
     1441 cccctcccgc cgaaaacgtc ccggttgcgc cgaaagatct cccgaaggcg acggcgcacg
     1501 cggttgcggg cgacggctcc cccgacccgc cgcgaaacag agagtcccag cctcggccgg
     1561 cccgccgatt ccgggtccgg ggaaggacga gccgtccccg ccgtcagcag aaaaacctgg
     1621 agatggcgtc cggagacgcg taccccggag gcgtagcact cttcgaattc ctgtcttttt
     1681 cgaaggcggt cgtcgcgaga gaacgtctcc cgccgacgat ccgggcccga gctcacaccg
     1741 aaagtttctt gcgtcccttg cgccgtcggg cggagatcac cgcgcggccc gagcgggtcc
     1801 gcatgcgcac gaggaacccg tgtgtgcgcc ggcgccgccg gttattgggc tggtaagtcc
     1861 gcttcataag aaacggcgca tcgtaggtgg ggaagggacc gatgtcaagg caaggggggt
     1921 caggtctcca cttgccactt tgctctcgca aactggtaag ggcgcagtgg gtttccgacc
     1981 gccggagcct ccagtgaaga cctgaccccg cattcctgta tactccgccg ccttccgggc
     2041 gaaagggctt tccacagctg cggaaaaact tgcggaaaac tacggtcgga gatgagtgag
     2101 atctggtcct ccatcgtcaa gaacctggag gggaagctcg acccgaagga gctgaagacc
     2161 tggttcgctc cgacccggca ggtctccttc gaccggcgcg gtgaggtggg cgctctcacc
     2221 gtggcggtgc cgagtcccgt cttcgccgag tggatcggcg cgcgacacag cgcccttctc
     2281 gcccgcgagg ccgcggcggt gggcttcccg gggctcgaca tccagttcct cccgatcgcg
     2341 gtcgagaccc cgatccctcc ggcgcccgtt ctcccccacc cgcgccggct cgtgctcaac
     2401 ccgcgattca ccttcgacac gttcgtcgtc gggtcttcga accagttcgc gcatgccgcg
     2461 gcgcgggccg tcggcgaatc gccgtcgcgc tcctacaatc cgctctttct ctacggcgga
     2521 gtcgggctcg ggaagaccca tctcatgcat gcgatcgcgc acgagatcct ccagcggtca
     2581 cccgaatcac gcgtggcata cctgtcggcc gagcggttcc tgaacgagct catcaacgca
     2641 ctgcgtttcg agaagatgca cgagttcaag agccgctacc gcgagctcga cgtcctcctc
     2701 atggacgaca tccagttcat cgccggcaag gactccacgc aggaggagtt cttccacacc
     2761 ttcaacgctc tccacgacgc gcagaagcag atcgtcgtca cctccgacgc gctgccgaag
     2821 gagattccga ctctcgagga gcggctgcgc tcgcgtttcg agtggggcct gatcgccgac
     2881 attcagccgc cggacctcga gatgaaggtg gcgatcatcc gcaagaaggc cgcggcggag
     2941 aaagcagaga tcccaaacga cgttgcgctc ttcatcgcgg ggacggtgaa gtcgaacatc
     3001 cgcgagctcg aagggcgcct gaaccgcgtc ctcgcgttct cgtcgctgac gggcaagccc
     3061 gtctccgtcg agttggcccg cgagacgctg cgcgagatcc tcggccacga ggaaaaccgc
     3121 gcgattccct cggaaatcgt gaaaaccgtg gcgtctcact acggtttgcg cgtgtcggat
     3181 ctgaaagcga agacgaacgc gaagccgatc gcgttcccgc gccaggtggc gatgtacctc
     3241 tgccggcggc tgacgggcct ctcctatccc gagatcggcc gtctcttcaa cgacaagcac
     3301 cactcgaccg tgatgcactc cgtcgagaag atcgaaaaga tggtcgagga cgatcccgat
     3361 ttccgcaaag tcatcgaggg gtttcagcag ccctatcgct gatccgcgac ctcacccggc
     3421 gcggttttcc gcttcactct tttccacatc tcccgcacgc cgcgagcgcg cgtggtgaca
     3481 agcgctcttt ccgctccggc gcgcaatggt gcgaggttcg tttcaggccc gtttttgttg
     3541 gcttttttta ctatcgatcc gcgcgtccac agtcctatta agacaactga atgtgataga
     3601 ctttttcttc agttcggagg tgggattccc gatggaattg acgctcaagc ggggcgattt
     3661 cttgaatgaa cttgcgctca tgcagggcgt cgtcgaacgg aagaacacca ttccgatcct
     3721 gtcgaacatc ctgctcaagg cgaccgacgg acatctcgat ctcacgtcga cggatctcga
     3781 cctgactctt ctctcccagg ccgaggcgaa agtctcgcgc ccgggcagcg tcacggttcc
     3841 ggcccggaag ctcttcgacc tgattcgcaa tctccccgag gccgacgtcc ggctgaaact
     3901 gatggagaac cactacctcg gcgtcacgtg cgagcggtcg aacttccggt tggtcgcgca
     3961 gcctgccgag gactatccga ccgtgccgaa ggtcgagggc aaggccggga tgacgtttcc
     4021 gctggaagcg tggaaacggt tgacccgcaa ggtcttatat gccgtttcgg ccgaggagac
     4081 acgcttccag ctgatgggcg cgctgctgaa ggacaagggc aaggagttcg agctcgtcgc
     4141 gaccgacggc caccgtctgg cgctcgtgaa tttcggcaag gagaacgtcc gcggcacgct
     4201 cccgaacgtt ctgatcccga aaaaagccct cgcggaaatc ctgaagatgg acggcggcga
     4261 gagcggcggc gagctcgaga tcaagcactc ggagaaccat ctcctgttcc gcgtcggcaa
     4321 ccggcagttg atcgcgagac ttctcgacgt caacttcccc gactacgaga agatcctcgg
     4381 caagggcaac gacaaggtcg ccatcgtccc gcgcgtggcg tgtcagtcgg cgatccgccg
     4441 cgttgcgctc ttctctcccg agcgcgcgcg gggcgttcga ctctcgctcg agaaagacct
     4501 gctgacgatc acctccgcga gccaggagct cggagacggc agcgagactc tcgacgtccg
     4561 atacgacagc gaaccgctct cgatcgggct gaacgcgcaa tacctcctcg atttcttcga
     4621 cgaggtggag acggaaaacg tgcgactgga gttgaaggac gagaacacgc agtgtctctg
     4681 tcatcccgaa ggagaggtcg a
//
LOCUS       DASWAV010000478         4422 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U2665
            k141_427747, whole genome shotgun sequence.
ACCESSION   DASWAV010000478 DASWAV010000000
VERSION     DASWAV010000478.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960316
            Sequence Read Archive: SRR10260007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 4422)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4422)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:47:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,929
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4422
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_427747"
                     /isolate="SMAG_U2665"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.36444 N 132.31889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..680
                     /locus_tag="VF958_09935"
     CDS             <1..680
                     /locus_tag="VF958_09935"
                     /inference="COORDINATES: protein motif:HMM:NF012382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEX9149468.1"
                     /translation="PTGVTASEAKRREILAAAVDAGALVVEDGYEEPESGKVPLAARD
                     PERVVWLGTLSKDLVPGFRIGWLAAPREVVERLALVKRTSDFQTPLPLQAAIASFLSA
                     GSDREARRARAWEVEVRREAMAAALARALPEVTWWGGEPGSAIFWLHLPKGVSGRRVA
                     EAAAARGVAVAAGADFDPQGEDRENLRLSVSRVDREAIGDGIAMLAEAVNEIRTRAQA
                     SVAAPVV"
     gene            691..1575
                     /gene="pdxS"
                     /locus_tag="VF958_09940"
     CDS             691..1575
                     /gene="pdxS"
                     /locus_tag="VF958_09940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014705692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0042823 - pyridoxal phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal 5'-phosphate synthase lyase subunit
                     PdxS"
                     /protein_id="HEX9149469.1"
                     /translation="MKESDSFRVKVGLAEMLKGGVIMDVVNAEQASIAQAAGAAAVMA
                     LERVPADIRRDGGVARMSPVTKIREIQEAVSIPVMAKCRIGHAAEARILEALKVDFID
                     ESEVLTPADEENHVYKHDFTVPFVCGCRNLGEALRRIGEGAAMIRTKGEAGTGDIVHA
                     VKHLRDVHRDIRNLTLMSKDERMHEAKRLQAPFELVEQVAETGRMPVPNFAAGGIATP
                     ADAALCMMLGAEAIFVGSGIFKSDDPERRARAIVKATTHWQDAQALVEAEESLGSGMK
                     GIDAASLPEEARMQGRGW"
     gene            1572..2216
                     /gene="pdxT"
                     /locus_tag="VF958_09945"
     CDS             1572..2216
                     /gene="pdxT"
                     /locus_tag="VF958_09945"
                     /EC_number="3.5.1.2"
                     /EC_number="4.3.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013568512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA];
                     GO_process: GO:0042823 - pyridoxal phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal 5'-phosphate synthase glutaminase
                     subunit PdxT"
                     /protein_id="HEX9149470.1"
                     /translation="MRAGLSQPPHPGPLLEGEGEEKGIAVLALQGDFAAHARRLAEMG
                     FGSFEARKPEEIRGASGLVIPGGESTTFWKFFEAAPWEKEIRDFAATGRPILGTCAGA
                     IVLAREVTNPTQRALGLIDIGVERNAYGRQADSFVGDVDAPELGGALPAVFIRAPRIR
                     RVGPDVEVLATREGEPVLVAHHNVIAATFHPELTSDTRVHELAFGQRRFARRSA"
     gene            2213..2965
                     /locus_tag="VF958_09950"
     CDS             2213..2965
                     /locus_tag="VF958_09950"
                     /inference="COORDINATES: protein motif:HMM:NF012596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HEX9149471.1"
                     /translation="MSVIRVDNREDGIAVVTLDRPRVNAIDSRLVDELHAAIRRLSGV
                     RALVLASSQPVFSAGWDLRALRGRTREEMVTFVSEFCDLVREIFSFPSPVVAALPGHA
                     IAGGLILAATADERIAADGNGLFGLSEVPLGVPLPRSLFEPFRHLLGVRGAERLAAAG
                     DNVSVERALQIGLVDRVVPPADLLEATIDRARQLCGRSRSAYAAVKLEARSEALERFD
                     CARRDDPFLDFWFSEEAQRRIGALVERLTKKS"
     gene            2962..4155
                     /locus_tag="VF958_09955"
     CDS             2962..4155
                     /locus_tag="VF958_09955"
                     /inference="COORDINATES: protein motif:HMM:NF012995.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016407 - acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arylamine N-acetyltransferase"
                     /protein_id="HEX9149472.1"
                     /translation="MTLPLDDVLDALELSREEPSLSFLEGLFTRFNARVPFETASKIL
                     RNVEVADSAGKPRWPDVFWRDFIERGTGGTCYARVAAFDALLSALGFETRKALGRVDE
                     DFDHAALFVAAGGRDWIADVGFPLPVLLPAGGGDVETEISSIAATASPRGVEVRFLSG
                     VPEGPRRLEIFRDRVSEDDFVELWRKTFRSDARFLSAVSLLRYDGPRVVKFARGEVLV
                     DDLHSRTSIPLLSVRPRKLAGLFGVDEDVLSRAFALAGDPEPEIRDARITAYLSTPGE
                     ARHAFEAIGTPEGYRRLMEGVADVAGEGWRLTFSAPGTAEVGFEEEITPDPERLTLAV
                     RRRYADGRELPLSFRVEVRDGETWLVREVVLSGPREDLLVNDSARGRLAGTLAVDLLA
                     WSRLI"
     gene            complement(4202..>4422)
                     /locus_tag="VF958_09960"
     CDS             complement(4202..>4422)
                     /locus_tag="VF958_09960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="3',5'-cyclic-nucleotide phosphodiesterase"
                     /protein_id="HEX9149473.1"
                     /translation="EVSFSDTQMDVARDSKHMTPALVAKELEKMPPRVPVYLYHMKPP
                     SLARIRREIAALREPRLKLLESERSFRF"
BASE COUNT          647 a         1457 c         1626 g          692 t
ORIGIN      
        1 acccgaccgg cgtgaccgcg tcggaagcga agcggcgcga gatcctggcc gcggcggtgg
       61 acgccggtgc gctcgtcgtc gaagacggat acgaggagcc ggagtcgggc aaagtgccgc
      121 tcgcggcccg cgaccctgaa cgcgtcgtgt ggctcggaac gctttcgaag gacctcgttc
      181 ccggtttccg catcggatgg cttgccgcgc cgcgcgaagt cgtcgagcgg ctcgcgctcg
      241 tcaagcgcac gtcggatttc cagaccccgt tgccgctcca ggcggcgatc gcgtcgttcc
      301 tctccgcggg atcggatcgc gaagcgcgcc gggcgcgcgc gtgggaagtc gaagtccgga
      361 gggaggcaat ggcggccgct ctcgcgcgcg ctctgccgga ggtgacgtgg tggggcggcg
      421 agcccgggag cgcgatcttc tggctgcacc tgccaaaggg ggtctcgggg cgacgcgtgg
      481 cggaagcggc ggcggcacgg ggggtcgccg tcgcggcggg cgcggacttc gatcctcagg
      541 gggaggaccg ggaaaacctg cggctgtcgg tctctcgcgt cgatcgcgag gccatcggag
      601 acggcatagc gatgctggcg gaggcggtga acgaaatcag aacgcgagcg caggcgtcgg
      661 tggctgcgcc cgtcgtttaa ggagaaaccg atgaaagaga gcgactcgtt tcgcgtgaaa
      721 gtcggattgg cggaaatgct gaagggcggc gtcatcatgg acgtcgtcaa cgcggagcag
      781 gcttcgatcg cgcaagccgc cggcgccgcg gcggtcatgg cgctcgagcg cgtgcccgcg
      841 gacatccgcc gcgacggggg agtcgcgcga atgtcgccgg tgaccaagat ccgcgagatc
      901 caggaggcgg tctcgatccc ggtgatggcg aagtgccgga tcggccacgc cgcggaagcg
      961 cgcatcctcg aggcgttgaa ggtggacttc atcgacgagt ccgaggtgtt gacgccggcc
     1021 gacgaggaga accacgtcta taagcacgac ttcacggtgc cgttcgtgtg cggctgccgg
     1081 aacctcggcg aggcgctgcg gcggatcggc gagggcgcgg cgatgatccg gacgaaaggg
     1141 gaggcgggca ccggcgacat cgttcacgcc gtgaagcacc tgcgcgacgt tcaccgcgac
     1201 atccgcaacc tgaccctgat gagcaaggac gagcgcatgc acgaggccaa gcgcctgcag
     1261 gcgccgttcg agctcgtgga gcaggtcgct gagaccggac ggatgccggt cccgaacttc
     1321 gccgcaggcg gaatcgcgac gccggcggac gccgcgttgt gcatgatgct cggcgcggag
     1381 gcaatcttcg tcggctcggg gatcttcaag tcggacgacc cggagcggcg ggcgcgcgcg
     1441 atcgtgaaag cgacgaccca ctggcaggat gcgcaggcgc tcgtcgaagc ggaggagtcg
     1501 ctcgggtccg gcatgaaggg gatcgacgcg gcatcgctgc cggaggaggc gcgcatgcag
     1561 gggagagggt ggtgagagca gggctcagtc agccccctca ccccggccct ctcctggagg
     1621 gagagggaga agaaaagggc atcgccgtcc tcgcgctcca gggcgacttc gccgcgcacg
     1681 cacggaggct cgcggagatg gggttcgggt cgttcgaggc gcggaagccg gaagagatcc
     1741 gcggggcgtc gggcctcgtc atacccggcg gcgagtccac gacgttctgg aagttcttcg
     1801 aggccgcgcc ctgggagaaa gagatccgcg acttcgcggc gaccggccgg ccgatcctcg
     1861 gtacgtgcgc cggcgcgatc gtgctcgcgc gcgaggtcac gaacccgact cagcgggcgc
     1921 tcggcttgat cgacatcggc gtcgagcgca acgcctacgg acgccaggcc gactccttcg
     1981 tcggcgacgt cgatgcgccg gagctcggcg gcgcgcttcc cgccgttttc atccgcgcgc
     2041 cccgcatccg ccgcgtcggg ccggacgtcg aggtcctcgc cactcgtgag ggggagccgg
     2101 tgctcgtcgc gcaccacaac gtgatcgccg caacgttcca cccggagctg acgtccgaca
     2161 cgagagtgca cgagctcgcg ttcggccagc gccgcttcgc ccggaggagc gcatgagcgt
     2221 catccgcgtc gacaaccggg aagacggcat cgcggtcgtc acgctcgacc ggccgcgcgt
     2281 gaacgcaatc gattccagac tcgtcgacga gcttcacgcg gcgattcgaa ggctttccgg
     2341 cgtccgcgcc ctcgtgctcg cctcgtccca gccggttttc tcggccgggt gggacctgcg
     2401 cgcgctgcgc gggcggaccc gggaggagat ggtgaccttc gtctcggaat tctgcgatct
     2461 cgtgcgcgag atcttctcgt tcccgtcccc ggtcgttgcg gcccttccgg gccacgcgat
     2521 cgccggcggc ctgatcctgg cggccaccgc tgacgaacgg atcgcggcgg acgggaacgg
     2581 cctcttcggg ctgtcggagg tccccctcgg cgttcccttg ccgcgttcgc tcttcgagcc
     2641 gttccggcac ctcctgggcg tccggggcgc cgagcgcctt gctgcggccg gcgacaacgt
     2701 ctccgtcgag cgcgccctcc agataggact cgtcgatcgc gtggttcctc cggccgacct
     2761 cctggaggcc acgatcgacc gcgcgcgcca gctctgcggg cggtcgcgct cggcctacgc
     2821 ggccgtcaag ctggaggccc gatccgaggc gctggagcga ttcgactgcg cccgtcgaga
     2881 cgatccgttt ctcgacttct ggttctccga ggaggcacag cggaggatcg gcgcactcgt
     2941 cgagcgtctg acgaagaaga gttgacgctt ccgctcgacg acgtcctcga cgcgctcgag
     3001 ctctcccgcg aggaaccttc gctttcgttt ctcgaaggac tgttcacccg gttcaacgcg
     3061 cgcgtgccgt tcgagacggc gtcgaaaatc ctgcgcaacg tcgaggtcgc cgattctgcc
     3121 gggaagccgc gctggcccga cgtcttctgg cgcgacttca tcgagcgcgg aacggggggg
     3181 acgtgttacg cgcgcgtggc cgccttcgac gcgctccttt ccgcgctggg gttcgagacg
     3241 cgcaaggcgc tcggcagggt cgatgaggac ttcgaccacg ccgcgctctt cgtcgccgcc
     3301 ggcggaaggg attggatcgc ggacgtcggg ttcccgctgc ccgtgctgct gccggccggc
     3361 gggggagatg tcgagaccga gatctcgtcg atcgcggcga cggcgagccc gcgcggcgtc
     3421 gaagtgcggt tcctctcggg cgtgccggag ggcccgcgcc gtctggagat cttccgagac
     3481 cgcgtctccg aggacgactt cgtcgagctg tggcggaaga ctttccgatc cgacgcgagg
     3541 ttcctctcgg ccgtctccct cctgcgatac gacggcccgc gcgtggtgaa gttcgcgcgt
     3601 ggagaagtgc tcgtggacga cctccactcc cggacgagca tcccgcttct gtccgtccgc
     3661 ccgaggaagc tcgcgggact cttcggggtc gacgaagacg tgctctcccg cgcgtttgcg
     3721 ctcgccggcg accccgagcc ggagatccga gacgcccgga tcacggcgta tctctcgacg
     3781 ccgggcgaag cgcggcatgc gttcgaggcg atcgggacac ccgagggcta tcggcggctc
     3841 atggaaggcg tggccgacgt ggcgggggag gggtggcgcc tgacgttctc ggcgccggga
     3901 acggcggagg tcgggttcga ggaagagatc acgccggacc cggagcggct cacgctcgcc
     3961 gttcgccggc gatacgccga cgggcgcgag ttgccgctct cctttcgggt cgaggttcga
     4021 gacggggaga cgtggctcgt tcgcgaggtc gtcctgtccg gccctcgcga ggacctcctc
     4081 gtcaacgact ccgcgcgcgg ccggctcgcc ggcacgctgg cggtggacct tctcgcctgg
     4141 tcgcggctga tctaaggcgg cgggtgcagg tccgcagacc tgtccccgcg actcttccag
     4201 ctcagaaccg gaagcttcgc tccgattcca ggagcttcaa gcgcggctcg cgcagggctg
     4261 cgatttcgcg gcggatgcga gcgagcgacg gcggcttcat gtggtacagg tacacgggca
     4321 cccgcggcgg cattttctcg agctccttcg cgacgagcgc cggcgtcatg tgcttcgagt
     4381 cgcgcgcgac gtccatctgc gtgtcggaga aggacacttc ga
//
LOCUS       DASWAV010000479         3798 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U2665
            k141_65485, whole genome shotgun sequence.
ACCESSION   DASWAV010000479 DASWAV010000000
VERSION     DASWAV010000479.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960316
            Sequence Read Archive: SRR10260007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 3798)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3798)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:47:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,929
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3798
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_65485"
                     /isolate="SMAG_U2665"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.36444 N 132.31889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(112..642)
                     /gene="ruvC"
                     /locus_tag="VF958_09965"
     CDS             complement(112..642)
                     /gene="ruvC"
                     /locus_tag="VF958_09965"
                     /inference="COORDINATES: protein motif:HMM:TIGR00228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008821 - crossover junction DNA
                     endonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HEX9149474.1"
                     /translation="MMILGLDPGTSATGFGSVLFDNGCLHLDRCGVWRPPDRLTLAAK
                     LDFLSGKLLALLEELRPAAVSVETVFAAKNVASTVKLAHARGVLLAAAAHFEIPVFEY
                     EPRLVKKAIVGYGHAEKPQVRSMVLTLLARHRDRVPLDAADALAVAICHAHASPAAPD
                     RGARSLPLASRRVSAK"
     gene            complement(729..1457)
                     /locus_tag="VF958_09970"
     CDS             complement(729..1457)
                     /locus_tag="VF958_09970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017874133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEX9149475.1"
                     /translation="MSGHSKWSTIKHKKGAADAKRGRLFTKLIREITMSARLGGGSAD
                     SNPRLRKAIDDAKAVNMPAENIKRAIQRGTGELPGVTYEEVTFEGYGPGGVAILIEAM
                     TDNKNRTLPEIRTIFAKHGGNLGESGSVKFLFQKKGYIAIEKDKATEDDVMEAAIEAG
                     AEDVGFTDHHHEVQTSPEMFPQVKAKLEERGLPLAVAELSMIPTTEVALDARKAEQTV
                     KLMEALEDHDDVQNVWANFDFEET"
     gene            1660..>3798
                     /locus_tag="VF958_09975"
     CDS             1660..>3798
                     /locus_tag="VF958_09975"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HEX9149476.1"
                     /translation="MAKNVLLIEYEQRDRRRVLSLLTPPDYSVTETNDGEEGLAAFAS
                     ERFDLILLSGKLPRMSANDVIRDIRKKGADAPPIVLLAAGYEGSNTKADAQKIGAFEI
                     VPKPFSDPALIGAVRSALESTDRGARTMKIPVSSKTLTSKDIFSDLLDELGRDTAAVP
                     ASAAAAAAPPAPAGTSPARRPSSAEEEIDRRLRDTLSGVTLPRRPSAGAPATSPSLQP
                     PVPTPLPRKADASPRASAANAEEIDRMISETLRIPVSKKPTAPPAAPRADATPTPTRR
                     AEPREAVAPSGPDRFGQYELLERIASGGMAELFRARRSGVEGFQKIVAIKKILPHIAG
                     NDEFITMFADEAKVAAQLNHPNIVHIYDLGKIEAGGYFIAMEYVEGTDLRAVLRSGRD
                     HDLQLPVPLAVYVASKVASALDYAHRRRDPRGEELKIVHRDVSPQNILISHEGEIKLC
                     DFGIAKADRKVSQTETGALKGKLQYMSPEQAWGKPIDHRSDLFSLGCVLHEMLTGERL
                     FRGDSDLTVLELVRKAEVPPPSRVNPDVPAALDAIVLKALAREPDDRYGTGSEMLRDL
                     ESILYSYTPAPGSADVAIYLNRLREAETGAAPGAPSEPPRPPRAMKDAPAAVAARATP
                     PTTPPEAKPPPSPASARPAETPATAEVFGSFSAARVDSEKKGRLPLYAGIAAVVVIGA
                     VLVFFLSRKGGGAPAAIAPTPAPVATAVPLE"
BASE COUNT          616 a         1318 c         1225 g          639 t
ORIGIN      
        1 ttactcggaa cgcgctggcc gccggcgagc gactcgccac ccacgactcg accgctcgcc
       61 tggttccttc gcggcgcgcc cgctcccgca tcgcgaagct cctccgctcg gttacttcgc
      121 ggagactctt cgcgatgcga gcggaagact cctcgcgccg cgatcgggtg ccgccgggct
      181 cgcgtgcgcg tggcagatcg cgacggcgag cgcgtcagcg gcgtcgaggg gaacgcggtc
      241 gcggtgacgc gcgagcagag tcagcaccat cgagcgaacc tgcggcttct cggcgtggcc
      301 gtatcccacg atcgcttttt tcacgagccg aggctcgtac tcgaagaccg gaatctcgaa
      361 gtgcgcggca gcagcgagca gcaccccgcg ggcgtgcgcg agtttcaccg tggaggcgac
      421 gttcttcgcc gcgaagacgg tttcgacgga gaccgccgcc ggccggagct cctccaggag
      481 ggccaggagc tttccggaaa ggaagtcgag cttcgccgcg agggtcaggc ggtcgggagg
      541 gcgccacact ccgcagcgat cgagatggag gcacccgttg tcgaagagga cggaaccgaa
      601 gcccgtggcc gacgttcctg gatcaaggcc gaggatcatc atggccggga aacgggaaac
      661 gggaaatggg aagcgatcgg atgcgggctc acgccgtggg cttcgttttc cgttttccgt
      721 cttgcgtctt atgtctcttc aaaatcgaaa ttcgcccaga cgttctgcac gtcgtcgtgg
      781 tcctcgagcg cttccatgag cttcaccgtc tgctccgcct tgcgcgcgtc gagcgcgacc
      841 tcggtcgtcg ggatcatgga gagctccgcg acggcgagcg gcagccccct ctcttcgagc
      901 ttcgctttca cctgcgggaa catctcggga gaagtctgaa cttcgtgatg gtggtcggtg
      961 aaaccgacgt cctccgcgcc ggcctcgatc gcggcctcca tgacgtcgtc ctcggtcgcc
     1021 ttgtcctttt cgatcgcgat gtagcccttc ttctggaaga ggaacttcac ggaacccgat
     1081 tctccgaggt tcccgccgtg cttcgcgaag atcgtgcgga tctcggggag ggtccggttc
     1141 ttgttgtccg tcatcgcttc gatcaggatc gcgacgccgc ccggtccgta tccctcgaac
     1201 gtcacctctt catacgtgac gcccggcagc tcgcccgtcc ctcgctgaat cgcgcgcttg
     1261 atgttctccg cgggcatgtt cacggctttc gcgtcgtcga tcgctttgcg cagacgcgga
     1321 ttggagtcgg ccgatccgcc gccgagtctc gccgacatcg tgatttcccg gatcagcttg
     1381 gtgaagagac gcccgcgctt cgcgtcggcc gcgcccttct tgtgtttgat cgtgctccac
     1441 ttgctgtggc ccgacatgag tgctcctcga aagaacggag tctgatcccg aagggtcgga
     1501 tcctgatttc tggcgcggcg attatattgc ccctctcttc gccgcggtgg cggcgtgacg
     1561 gcacgcttcg aagtcgagaa aacccctgtc tgcgttggct tttctgcgcg cgatgccgta
     1621 tcatggaact gcttttcccg gcgacagctg actaatcgga tggcaaaaaa cgtactcctg
     1681 atcgaatacg aacagaggga ccgcaggcgt gtattgtccc tgctgacccc gccggattat
     1741 tccgtcaccg aaacgaacga cggcgaggaa ggtctcgccg ccttcgcctc cgagcggttc
     1801 gacctcatcc ttctctccgg aaagctgcca cggatgtcgg cgaacgacgt catccgtgac
     1861 atccggaaga agggggccga cgcccccccg atcgtcctgc tggccgccgg atacgagggg
     1921 tccaacacga aggcggacgc ccagaagatc ggcgccttcg agatcgtccc caagccgttc
     1981 agcgacccgg ccctgatcgg cgcggtgcgg tccgccctcg agtcgaccga ccgcggggcg
     2041 aggacgatga agatccccgt ctcgtcgaag acgctcacgt ccaaggacat cttctccgac
     2101 ctcctggacg agctcggacg cgacaccgcc gcggtccccg cgagcgcggc cgcggccgca
     2161 gcgcccccgg caccggcggg cacttctccg gctcgccgcc cgtcgagcgc cgaggaggag
     2221 atcgaccgaa ggctgcggga cactctctcc ggagtgacgt tgcccagacg cccgagcgcc
     2281 ggcgcccccg caacttcccc gagcctgcag ccgccggtcc cgacccctct ccctcgaaaa
     2341 gctgacgcgt cgccccgggc gtccgcggcg aacgcggaag agatcgaccg gatgatctcc
     2401 gaaacgctgc gcatcccggt ttcgaagaag ccgaccgcgc cgccggcagc gccccgcgcg
     2461 gatgcgacgc cgacgccgac gcggcgcgcc gagcctcggg aagcggtcgc tccgtcgggc
     2521 cccgaccgct tcgggcaata cgagcttctc gagaggatcg cgagcggcgg gatggcggag
     2581 ctcttccgcg cccgccggag cggcgtcgaa ggcttccaga agatcgtcgc gatcaagaag
     2641 atccttccgc acatcgctgg caacgacgag ttcatcacga tgttcgccga cgaggcgaag
     2701 gtcgctgcgc agctcaatca ccccaacatc gtgcacattt acgacctcgg aaagatcgaa
     2761 gcgggcgggt acttcatcgc gatggagtac gtggagggaa cggacctgcg tgcggtcctc
     2821 cggtccggcc gcgaccacga cctccagctc cccgtgccgc tcgcggtcta cgtggcaagc
     2881 aaggtggcgt ccgcgctcga ctacgcccat cgccgccgcg atcctcgcgg cgaagagctc
     2941 aagatcgtcc accgcgacgt gtcgcctcag aacatcctca tctcgcacga gggggagatc
     3001 aagctctgcg acttcggcat cgccaaggcc gaccggaagg tgtcgcagac cgagaccgga
     3061 gctctgaagg gcaagctcca gtacatgtcg cccgagcagg cgtggggcaa gccgatcgat
     3121 caccggagcg atctcttctc gctcggatgc gtcctccacg agatgctcac gggggagcgg
     3181 ctctttcgcg gcgattcgga cctgacggtg ctcgagctcg tgcggaaggc cgaggtgccg
     3241 ccgccatcga gggtgaaccc ggacgtgccg gccgcgctcg acgcgatcgt cctgaaggcc
     3301 ctcgcccgcg aacccgatga ccgctacggg accgggtctg agatgctccg cgacctcgag
     3361 tcgatcctgt actcctacac gccggcgccc gggagcgcgg acgtcgcgat ctatctgaac
     3421 cgcctccgcg aggcggaaac cggcgccgcc ccgggcgctc cgtccgagcc gccgcgtccg
     3481 cctcgagcga tgaaggacgc tcccgcagcg gtggccgccc gggcgacgcc cccgacaaca
     3541 ccgccggaag cgaagccgcc gccttcgccg gcaagtgcgc gtcccgcgga aacaccggca
     3601 acggccgaag tcttcgggtc gttctcggcg gcgcgggtcg attccgagaa aaaaggccgg
     3661 ctcccgctct atgcgggcat cgccgcggtg gttgtgatcg gcgccgttct cgttttcttc
     3721 ctcagccgca agggcggcgg cgcgcccgcg gcaatcgctc cgacgcccgc tccggtcgcg
     3781 accgcggttc ctctggag
//
LOCUS       DASWAV010000480         2333 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U2665
            k141_106666, whole genome shotgun sequence.
ACCESSION   DASWAV010000480 DASWAV010000000
VERSION     DASWAV010000480.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960316
            Sequence Read Archive: SRR10260007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2333)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2333)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:47:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,929
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2333
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_106666"
                     /isolate="SMAG_U2665"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.36444 N 132.31889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1464
                     /locus_tag="VF958_09980"
     CDS             <1..1464
                     /locus_tag="VF958_09980"
                     /inference="COORDINATES: protein motif:HMM:NF016693.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan 7-halogenase"
                     /protein_id="HEX9149477.1"
                     /translation="FDWKVGESTVEVSAYFLTRVLKQYDHLSREQLPKQAFRYWFSNS
                     DVTSLREASEVGPTQLARTPSFQLDRAKLDEHLLNVAAQEGAEVWRPARVTGFELGGE
                     AGNTVSIEKADGASVTVRSRWVVDATGRHAMLARRRGGVTPIESHPTSAIWVRYRGVK
                     DLDGVEVAGTDPADPYARAVLASRRLATNHFTGWGYWIWFIPLSGGETSVGLVWDKRL
                     VTPEGKTPLEKLTRFLDGNPLTRELVEHAEPIAQDCRFYGHLPYFVDRFIGPGWTCVG
                     DAGGFLDPFYSPGLDQMAFSVHTRVELMRRALGGEAPESMARAYVDHNEKYARYFHYF
                     YESIYRDKYYVMGDYDTMTTAFLLDTALYYAAAVMPIYRWNADRLGFPPFYQDGAEVG
                     FYPIRFYNRRLAAIAKRKMALGIYGNHNAGRRPSFVGFSVRSAMFVMLAHGLLRWWKA
                     ELANAWTYVMKPRRLGQPAAANPPVASASDSSRASAR"
     gene            1478..2278
                     /locus_tag="VF958_09985"
     CDS             1478..2278
                     /locus_tag="VF958_09985"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018131018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX9149478.1"
                     /translation="MPGTTLVTGASSGIGREIAWLAAKERRDVVLVGRRRERLELFAG
                     ELVARFGVAADVVVADVAEPDSPARIFAAAEQRGPVEVLVNAAGLGVHGFFWKTPFEQ
                     ERETILVNVLALTELTKLCVPGMVERRRGRILNLASTAAFQPGPLMAVYYATKAYVLS
                     FTEALAEELRGTGVTATALCPGPVLTGFQEKAGLADVPLLRGPIVLDVAKVARAGWRG
                     AMRGKRVVVPGIANRVLAAGARFSPRRLSTKIARRLQENRKPRGRLLN"
BASE COUNT          362 a          793 c          798 g          380 t
ORIGIN      
        1 ttcgactgga aggtcggaga gtcgacggtc gaggtctccg cctacttcct gacgcgcgtc
       61 ctcaaacagt acgaccacct ctcgcgcgag cagctgccga agcaggcctt ccgatactgg
      121 ttctccaact cggacgtcac cagcctccgc gaggcgtccg aggtcgggcc gacgcagctc
      181 gcgcgcaccc cgtccttcca gctcgaccgc gcgaagctcg acgagcacct tctcaatgtc
      241 gcggcgcagg agggcgccga ggtgtggcgg ccggcgcggg tcaccgggtt cgagcttggc
      301 ggcgaagccg gcaacacggt ttccatcgag aaggcagacg gcgccagcgt gaccgtgcgc
      361 tcccggtggg tcgtcgacgc gaccggccgg cacgccatgc tcgcgcggcg gcggggtggc
      421 gtgacgccga tcgagtccca tccgacatcc gccatctggg tgcggtatcg gggcgtcaaa
      481 gacctcgacg gagtggaggt cgcgggcacg gaccccgcgg atccgtatgc gcgcgccgtg
      541 ctcgcctctc gccgcctcgc cacgaaccac ttcaccgggt ggggctactg gatctggttc
      601 atcccgctct cgggcggcga gacgagtgtc gggctcgtct gggacaagcg actcgtgacg
      661 cccgagggaa aaacgccgct cgagaagctc actcgattcc tcgacggcaa cccgctcacg
      721 cgggagctcg tcgagcacgc cgagccgatc gcgcaggact gccgcttcta cggccacctc
      781 ccctacttcg tggatcggtt catcggaccg ggctggacgt gcgtcggcga cgccggtgga
      841 ttcctcgacc cgttctactc gccgggtctc gatcaaatgg ccttctccgt ccacacgcgc
      901 gtcgagctca tgcgccgggc gctcggaggc gaggcgcccg aatcgatggc gcgcgcctac
      961 gtcgatcaca acgagaagta cgcgcgatat ttccactact tctacgagtc gatctaccgc
     1021 gacaagtact acgtgatggg cgattacgac acgatgacga cggcgttcct gctcgacacg
     1081 gcgctctact acgctgccgc cgtgatgccg atctaccggt ggaacgcgga ccgcctgggc
     1141 ttcccgcctt tctaccagga cggcgcggag gtcggcttct atccgatccg gttctacaac
     1201 cgcaggctcg ccgcgatcgc gaagaggaag atggcgctcg ggatctacgg aaaccacaac
     1261 gcaggaagga ggccgagctt cgtcggcttt tccgtgcgca gcgcgatgtt cgtgatgctc
     1321 gcgcacgggc tcctccgctg gtggaaggcg gagctcgcga acgcctggac gtacgtgatg
     1381 aaaccccgaa ggctcggaca acccgcggcc gcgaatccgc cggtcgcgtc cgcgtccgac
     1441 tcttctcgcg cctcggcccg atagctccgc gaaacggatg cccggaacga ctctcgtcac
     1501 cggcgcatcg agcgggatcg gacgggagat cgcctggctc gccgcgaagg agcgccgcga
     1561 cgtcgtgctc gtggggcggc gccgggagag gctcgagctt ttcgcgggag agctcgtggc
     1621 gcgcttcggg gtcgccgcgg acgtcgtggt cgctgacgtc gcggaacccg actctcctgc
     1681 gcggatcttc gccgcggcgg agcagcgtgg ccccgtcgag gtgctcgtca acgcggcggg
     1741 actcggcgtc cacggcttct tctggaagac cccgttcgag caggagcgcg agacgattct
     1801 cgtcaacgtg ctcgcgttga ccgagctgac gaagctctgc gtccccggga tggtcgagcg
     1861 gcgccgcggg cggatcctga acctcgcgtc gacggccgcg ttccagcccg gcccgctcat
     1921 ggccgtctac tacgcgacga aggcctacgt cctctcgttc accgaggccc tcgccgagga
     1981 gttgcgcggg acgggcgtca ccgcaactgc gctctgcccc ggacccgtcc tgaccgggtt
     2041 ccaggagaag gcgggcctgg ccgacgtacc gctcctgcgt ggaccgatcg tcctcgacgt
     2101 cgcgaaggtc gcgcgcgcgg ggtggcgagg cgcgatgcgc ggaaagcgcg tcgtcgtccc
     2161 gggcatcgcg aatcgagtcc ttgcggccgg cgcgcgtttc tcgccgcgcc ggctttcgac
     2221 gaagatcgcg cggaggctcc aggagaaccg gaagccgcga ggtcggctgc taaactgatt
     2281 ttctcggagg agtggccgag tggtttaagg cggcggtctt gaaaaccgct gta
//
LOCUS       DASWAV010000481         7429 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U2665
            k141_507171, whole genome shotgun sequence.
ACCESSION   DASWAV010000481 DASWAV010000000
VERSION     DASWAV010000481.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960316
            Sequence Read Archive: SRR10260007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 7429)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7429)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:47:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,929
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7429
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_507171"
                     /isolate="SMAG_U2665"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.36444 N 132.31889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..221
                     /locus_tag="VF958_09990"
     CDS             <1..221
                     /locus_tag="VF958_09990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004513521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX9149479.1"
                     /translation="EDLPVEMRESIGEGIAPGAPLREARDDFERRYILAALKRYRGNV
                     SRTAEALDLERSNLYRKLKTYGIEVERE"
     gene            complement(245..1144)
                     /gene="ftcD"
                     /locus_tag="VF958_09995"
     CDS             complement(245..1144)
                     /gene="ftcD"
                     /locus_tag="VF958_09995"
                     /EC_number="2.1.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007662187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030409 - glutamate formimidoyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0030412 - formimidoyltetrahydrofolate
                     cyclodeaminase activity [Evidence IEA];
                     GO_process: GO:0019556 - histidine catabolic process to
                     glutamate and formamide [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate formimidoyltransferase"
                     /protein_id="HEX9149480.1"
                     /translation="MRAVIECVPNISEGRNPEVVSEIAAAVRSAPGVRLLDVSSDPAH
                     NRSVLTFVADGPGVRAGVRALFEAAIPRIDLTRHRGEHPRMGAVDVVPVIPIRGATVE
                     ECVALSREIAAEIGDRHRVPVYLYEDSATAERRRNLAEIRKGEFEGFAEKIRLPEWKP
                     DFGPDAPHPTAGVVAVGARAPLIAYNINLATRELAVANRIAKAIRHLGGGFRYVKALP
                     VELADRGQVQVSINMTNFKKSPLHRVFECVKSEAERHGVAIVGSEIVGLTPAEALFMA
                     AEHYLRLEGFSSDQVLETRLLEE"
     gene            1245..3149
                     /locus_tag="VF958_10000"
     CDS             1245..3149
                     /locus_tag="VF958_10000"
                     /inference="COORDINATES: protein motif:HMM:NF024579.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX9149481.1"
                     /translation="MSSDAPSGGYPGNLSLPVEVREKILSTFRHTLDLYRDGKVDDCL
                     IGCDFILKMDPRFAPARKLQEKAKNPKAAIDLAELQSVTSPGSPPPSPRVTGNVVPAR
                     ETGGTVPPRRDTAPVQPPAASPFLSQGGEPAGMDATASNDLEELSLDSLSLDGPLPDL
                     AAKAGTHEAGLPFGPGSSDADFPGLSQDPFAPDPGLAAASALSPTEEIEILLRKGDEA
                     RSSGDKQQAIEIWSRVFLIDINNAEAVTRIEDARREMVEESRKVAAALERGQESFAKG
                     DLASARSEFDSVLSVGAEPPATAPPVAPVAKTPPPAALPAHDLSAVAAPGDVLAEEMD
                     QSGPPGGRRSWTSIPRPPRDSAPDTAEPAAKRPGLRIPRIPPRVALIAGAGVVVVAAA
                     MFFFLRSPGERTVPPRSESVPSLEHATQLFQEGKIDETTEELRRIPRGSPDYDRAQKL
                     LASLDGSKESAPRPAAGSGPAVAGDAAAGDPASLRADAERALAENRYIDAMKSFSLAA
                     PHFRGDPTFSQEMAVASERVSELTPAVKLYNDGEYETAIPILWRLYQASRDNKDARSY
                     LVRSYYNQGILELQNGLFDKAKESFGEVLSLDPQDVETARHRQFAEKYHSGELDLLGR
                     IYVRYISPRP"
     gene            3146..3703
                     /locus_tag="VF958_10005"
     CDS             3146..3703
                     /locus_tag="VF958_10005"
                     /inference="COORDINATES: protein motif:HMM:NF018028.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HEX9149482.1"
                     /translation="MSGPPEPPSLSAPFNPEAERALNDFPLRPEPPLDLGSTDASETP
                     VAAPASGLAFLPDRAIGAAADVATSILVVVVALLAAFAARDRTPRISGLGWAALFALT
                     VSFFAVVVPLVLFGRTVGMALAGLVAKDEGGGRRLTPTQAVRRWAGTLITLAGAGLPL
                     LWSLRDAGAPTPADRLSGRTLVRDG"
     gene            3700..5355
                     /locus_tag="VF958_10010"
     CDS             3700..5355
                     /locus_tag="VF958_10010"
                     /EC_number="6.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013580097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003952 - NAD+ synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD+ synthase"
                     /protein_id="HEX9149483.1"
                     /translation="MRTRSLRVAVAQIDTTVGDFRGNSEKIVAYGRRAEERGADLVLF
                     PELAVCGYPPKDLLDRRSFLREAEHAADRIARASGTATWLYGAVLSNRARTGRAVFNA
                     AVAARRGKRVGEYHKRLLPTYDVFDEGRYFEPGRRPPLVLRVKGMRVGVTICEDIWND
                     KTFWKRPLYPHDPVGDLKPLELDLHANISASPYTLGKDRLRRRFMAQIARRTNVPLVV
                     CNLVGGNDGLVFDGGSAAFDSRGRLIGRGLSFQEDFWTIDLPDGRGPSTPSESDVAAL
                     SRALVLALSDYAGKCGFSEAVLGLSGGVDSAVTAALAAQALGSEKVLGVAMPGPYSSP
                     GSVRDARELARRLGIRLIEIPIGPVFEAYRRVLAPAFGPGDCIAAEENLQARIRGALL
                     MGLSNRFGYLVLSTGNKSEIAVGYSTLYGDMAGGYALISDVPKTLVYELAREINREEE
                     KIPEATLKKEPSAELRPNQKDTDTLPPYVELDPLIEALVDLSLPVDLAARRAGVSQKL
                     AREIARRVDRNEYKRRQMPPGPKVTAKAFGEGRRYPIAHRFLV"
     gene            5348..6073
                     /locus_tag="VF958_10015"
     CDS             5348..6073
                     /locus_tag="VF958_10015"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX9149484.1"
                     /translation="MSDPTGSAPGSGERPAGSGEKYVWKEIPGSSHDLLRRRILALPS
                     GLRLLDLGAAGGHLGRAVRARCSYLAGVEPDPTLPASAREGYDDWRQIDALNAGDWDR
                     AFDVVVCADVLEHLSRPEAMLARIHEWLHPEGIFLASLPNIANVSIRASLLFGKFRYT
                     DRGILDRSHVSFYTRSSALRLLEDSGFRVTSVEPTAMPYELAVPALSAAPMVRPVRGF
                     AQASARMWPTLFGYQFVFQAIRR"
     gene            6070..6846
                     /locus_tag="VF958_10020"
     CDS             6070..6846
                     /locus_tag="VF958_10020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012529483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dolichyl-phosphate beta-glucosyltransferase"
                     /protein_id="HEX9149485.1"
                     /translation="MSRLETGGRASLVVPAFDEARRIERSMAEIAAWRRSRPSGWNWE
                     VILVDDGSNDGTGELARRAAEKEGLPVTQLRHDRNRGKGAAIRTGVLASTGDPVLVTD
                     TDLSTPLAEWVKLAERLPTHPVTIGSRAIDEDLVRRRQPFYRQFMGKSFNTFVRLLAV
                     PGIRDTQCGFKLFRGDVARELFSEARIDRFAYDVEILYLARRAGIPIAEVPVLWFNSP
                     ESKVAVVKDSLRMLRDLLRIRWIHRGRKTGNRKREAKEVR"
     gene            complement(6843..>7429)
                     /locus_tag="VF958_10025"
     CDS             complement(6843..>7429)
                     /locus_tag="VF958_10025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9149486.1"
                     /translation="ERADRGASRRLALASLACVGTKNEGLFFVAAGVVVAALFAFSRR
                     GVVARAAAILALPAALVVAAHRLIRGSLPLSDFDFSLLRRPGELAPRIGESLTAVFRL
                     TPQLAWAGLVALAVLVAAGRPSPAGGRLLALAACGLSAYLFLPSLAVAGPGWLVETAF
                     FRTTAALAPLVAAGVAARLIPLFEKRETGNGKGL"
BASE COUNT         1178 a         2583 c         2509 g         1159 t
ORIGIN      
        1 ccgaggatct tcccgtcgag atgcgggaat cgatcggcga ggggatcgct ccgggcgcgc
       61 cactgcgcga ggcacgcgac gacttcgagc gccgctacat tcttgccgcc ctgaaacggt
      121 accgcggcaa cgtatcgcgt accgccgagg ctctcgacct cgagaggtcg aatctctaca
      181 ggaagctgaa gacctacggg atcgaagtgg agagggagta gcggcgggtc gcgagcgact
      241 ccacctactc ctccagcaat cgcgtctcca gcacctgatc gctcgagaac ccctcgaggc
      301 gcaggtagtg ctcagcggcc atgaaaagcg cctctgccgg agtcaggccg acgatctccg
      361 atcccacgat cgcgacgccg tggcgctccg cctcggactt cacgcactca aacacccggt
      421 ggagcgggct cttcttgaag ttcgtcatgt tgatcgaaac ctggacctgc ccgcgatcgg
      481 caagctccac cggaagggcc ttcacgtaac ggaatcctcc cccgagatgc cggatcgctt
      541 tcgcgatgcg attggcgacg gcgagctcgc gggtcgccag gttgatgttg taggcgatga
      601 gcggagcgcg tgcgccgacg gccacgacgc cggccgtcgg atgcggcgcg tccggcccga
      661 aatccggctt ccactccggc agccggatct tctcggcgaa gccctcgaac tcccccttgc
      721 ggatctcggc cagattgcgc cggcgttcgg cagtcgccga atcctcgtag aggtagaccg
      781 ggacgcgatg gcggtctccg atctctgccg cgatctcgcg cgagagcgcg acgcactcct
      841 cgaccgtggc gccccggatc gggatcaccg gaacgacgtc caccgcgccc atccgggggt
      901 gctcgccgcg gtggcgggtg agatcgatcc tcggaatggc ggcctcgaac agagcccgta
      961 cgcccgcccg aacgccgggc ccgtccgcga cgaacgtgag gacgctgcgg ttgtgggccg
     1021 ggtcggagga gacgtcgagc aggcgcacgc ccggcgccga ccgaacggcc gccgcgatct
     1081 cggacacgac ttccggattg cgcccctcgg agatgttcgg gacgcactcg atgaccgccc
     1141 tcatggggaa ttccctctcc gcgcagttta taatttccaa tgatcgtgac gttgtcgaac
     1201 ggagaaacac acggggcggc ggcgcccgcc cccgaggggg acctgtgagc agcgacgcgc
     1261 cttccggcgg ctatccgggg aacctgtcgc tgccggtcga ggttcgggag aagattcttt
     1321 cgaccttccg tcacacgctc gatctgtacc gcgacggaaa agtcgacgac tgcctgatcg
     1381 ggtgcgattt cattctcaag atggatccgc gcttcgcgcc tgcgcgaaaa ctgcaggaga
     1441 aggcgaagaa cccgaaggcc gcaatcgatc tagccgagct gcagtccgtg acctctcccg
     1501 gctcgcctcc tccgtcgccg cgggtgaccg gaaatgtcgt gcccgcgcgc gagaccggag
     1561 gaaccgttcc gccccgccgg gacacagcgc cggttcaacc tccggccgcc tctcctttcc
     1621 tttcccaggg cggtgaaccg gcgggaatgg acgcgaccgc atccaacgat ctcgaggagc
     1681 tctccctcga ttcgctgtcc ctcgacggtc cccttcccga cctggcggcg aaggcgggaa
     1741 cccacgaggc gggtctcccg ttcgggcccg gatcctccga cgcggatttc cccggactct
     1801 cgcaggaccc cttcgcgccg gaccccgggc tcgccgctgc cagcgctctc tctcccacgg
     1861 aggagatcga gatactcctc aggaaagggg acgaggcgcg gagctccggg gacaaacagc
     1921 aggcgatcga gatctggtcc cgcgtcttcc tgatcgacat caacaatgcg gaagccgtca
     1981 cgcggatcga agacgcgcgc cgggagatgg tggaggaaag tcggaaagtc gcggccgcgc
     2041 tcgagagagg ccaggaaagc ttcgcgaagg gcgacctggc ttcggcccgc agcgagttcg
     2101 attccgttct gtctgtcggg gctgagcctc ctgcgacggc gccccccgtc gcacccgtgg
     2161 caaagacgcc gcctccggcg gcccttcccg cgcacgacct ttccgccgtc gccgcgccgg
     2221 gtgacgtcct cgcggaggag atggatcagt ccggcccgcc cggcggtcgt cgctcgtgga
     2281 cgtccatccc gcgcccgcct cgcgacagcg cgccggacac cgcggagcct gctgcgaaac
     2341 gtccggggct tcggattcct cggattccgc cgcgcgtcgc gctcatcgcg ggagcggggg
     2401 tcgtcgtcgt cgcggctgcg atgttctttt tcctccgctc cccgggggag cggacggttc
     2461 cgccgcgcag cgagtccgtg ccttccctcg agcacgcgac gcagctcttt caggagggca
     2521 agatcgacga aacgacggaa gagctccggc ggataccgcg cggaagtccg gactacgacc
     2581 gcgcccagaa gctcctcgca tcgctggacg gatcgaagga gtccgcgccc cgaccggccg
     2641 cgggaagcgg accggccgtc gcgggcgacg cggccgcggg ggatccggca tccctccggg
     2701 cggacgccga aagagcactc gcggaaaacc gatatatcga cgcgatgaag agcttcagcc
     2761 tcgccgcccc ccatttccgg ggggacccca cgttctcgca ggagatggcc gtcgcctccg
     2821 agagggtgag cgagctgaca ccggcggtga agctctacaa cgacggcgag tacgagaccg
     2881 cgatcccgat cctctggcgg ctctaccagg cgtcgcgcga caacaaggac gcccgttcgt
     2941 acctcgtgcg gtcgtactac aaccagggga tcctcgagct ccagaacggc ctcttcgaca
     3001 aggcgaagga gtcgttcggc gaggtgctct ctctcgatcc gcaggacgtc gagacggcgc
     3061 ggcaccgtca gttcgccgag aagtaccact cgggcgagct cgaccttctc ggccgcatct
     3121 acgtgcggta catctcgccg cgcccgtgag cggtccgccg gagccgccta gcctttcggc
     3181 ccctttcaac ccggaggccg agcgagcgct gaacgacttt ccgctccggc cggagcctcc
     3241 gctcgacctg ggatcgaccg acgcttccga gacgcctgtc gccgcacccg catccggcct
     3301 ggccttcctg cccgaccgcg cgatcggcgc agcggccgac gtggcgacgt cgattctcgt
     3361 cgtggtcgtc gccctgctcg cggccttcgc ggcacgggac cgcacgccgc ggatctcggg
     3421 gctcggatgg gcggcgctct tcgcgctcac cgtttccttc ttcgccgtcg tcgtgcctct
     3481 cgtcctcttc ggccgtaccg tcggcatggc gctcgccggg ctcgtcgcga aggacgaggg
     3541 gggcggccgc cgcctgacgc ccacgcaagc ggtgcgtcgg tgggcgggga cgctgatcac
     3601 cctcgccggc gcgggcctcc ctctcctctg gagcctgcgc gacgccggcg ctccgacccc
     3661 ggccgaccga ctgtccggac ggacgctggt ccgcgacggt tgagaacgcg ctccctgcgc
     3721 gtcgccgtcg cgcagatcga caccacggtc ggagacttcc gcggaaactc cgagaagatc
     3781 gtcgcgtacg gtcgccgggc ggaggagcgc ggggccgacc tggtgctctt tccggagctc
     3841 gccgtgtgcg gttatccgcc gaaagacctc ctggatcggc gatccttcct gcgtgaggcg
     3901 gagcacgccg ccgatcggat cgcccgagcc tccggtaccg cgacctggct ctacggagca
     3961 gtcctctcca accgtgcgcg cacgggccgg gcggttttca acgcggccgt cgcggcgcgc
     4021 cggggaaaac gcgtcggcga gtatcacaag aggctgctgc ccacgtacga cgtgttcgac
     4081 gaggggcgct acttcgagcc ggggcggcgg ccgcccctcg tcctgcgggt gaaagggatg
     4141 cgggtcggag tcacgatctg cgaggacatc tggaacgaca agacgttctg gaaaaggcct
     4201 ctttatccgc acgatccggt cggcgacctg aagcccctcg aactcgacct tcacgcgaac
     4261 atctccgcgt cgccttacac gctcggcaag gaccggcttc gaagacggtt catggcgcag
     4321 atcgcccggc ggacgaacgt cccgctcgtc gtctgcaacc tcgtcggcgg aaacgacggc
     4381 ctcgtgttcg acggcgggtc cgccgcgttc gactcgcgtg gccggctcat cgggcgcggt
     4441 ctctcgttcc aggaggactt ctggacgatc gaccttccgg acgggcgcgg cccttcgacc
     4501 ccgtcggaat cggacgtcgc ggccctctcc cgtgcgctcg tcctcgccct ctccgactac
     4561 gcgggcaagt gcggcttctc cgaggccgtg ctcgggttgt cgggcggcgt ggactcggcg
     4621 gtgacggcgg cgctcgcggc gcaggcgctc gggagcgaaa aggtcctcgg cgtggcgatg
     4681 ccggggccgt actcgtcgcc cgggagcgtc cgggacgcgc gggagctggc ccggcgtctc
     4741 gggatccgtc tcatcgagat tccgatcggc ccggttttcg aagcttaccg tcgcgtgctg
     4801 gcgcccgcgt tcgggccggg agactgcatc gcggcggagg agaacctcca ggcgcgcatc
     4861 cgcggagcgc tgctcatggg cctctcgaac cgattcggct atctcgtgct gtcgacggga
     4921 aacaagtcgg agatcgcggt cgggtactcc actctctacg gcgacatggc gggaggctac
     4981 gccttgatct ccgacgttcc gaagacgctc gtctacgagc tcgctcgtga gataaatcgg
     5041 gaggaggaga agattcccga ggcgactttg aagaaggagc cctcggccga gctgcgcccg
     5101 aatcagaagg acacggacac tcttcccccc tatgtcgaac tcgatcctct catcgaggcg
     5161 ctcgtcgacc tttcgttgcc ggtggatctc gcggcgcgaa gggcgggcgt ctcgcagaag
     5221 ctcgcgcgtg agatcgcgcg gcgagtcgac cgcaacgagt acaagcgccg gcagatgcct
     5281 cccggtccga aagtcacggc gaaggccttc ggcgaaggac ggcgctatcc catcgcccac
     5341 agattccttg tctgatccga cgggaagcgc cccgggttcc ggagagaggc cggcggggtc
     5401 gggagagaag tacgtctgga aggagattcc cggctcttcc cacgacctgc ttcgccgccg
     5461 aatcctcgcc ctcccctccg gactccggct cctcgatctc ggcgcggcgg gaggccacct
     5521 cgggcgcgcc gttcgagccc ggtgctccta tcttgccggc gtcgagccgg acccgactct
     5581 cccggcgtcg gctcgcgagg gatacgacga ctggcgacag atcgacgccc tcaatgcggg
     5641 cgactgggac cgggcgttcg acgtcgtcgt ctgcgccgac gtcctcgaac acctctcgag
     5701 acccgaggca atgctcgcgc gaatccacga gtggctccac ccggaaggga tctttctcgc
     5761 gtcgctgccg aacatcgcga acgtctcgat ccgcgcctcc ctcctgttcg ggaagttccg
     5821 ttatacggat cgcggcatac tcgatcggtc ccacgtctcc ttctacacgc gctcgagcgc
     5881 gctgcggctt ctcgaggact cggggttccg ggtcacctcc gtggaaccca cggcaatgcc
     5941 gtacgagctg gcggtgccgg cgctctccgc cgctccgatg gtccgtcccg tgcggggttt
     6001 cgcacaggcg agcgcgcgga tgtggccgac gctcttcgga taccaattcg tcttccaggc
     6061 gatccggcgg tgagccggct cgagaccgga ggtcgggcgt cgctcgtcgt gcccgccttc
     6121 gatgaggcga ggcgcatcga gcgctcgatg gccgaaattg cggcctggcg tcggtcgcgt
     6181 ccgagcgggt ggaactggga ggtcatcctc gtcgacgacg gctcgaacga cggcaccggc
     6241 gaactcgcgc gacgggcggc cgagaaggaa ggcctcccgg tcacgcagct ccggcacgac
     6301 cggaaccggg gaaagggcgc cgcgatccgg accggagttc tcgcgtccac gggcgacccg
     6361 gttctcgtga ccgacacgga cctctcgacg ccgctcgcgg agtgggtgaa gctcgcggag
     6421 cggctgccga cccatccggt cacgatcggg tcgcgcgcga tcgacgagga cctggtgcga
     6481 agacgccagc ctttttaccg ccagttcatg ggcaagtcct tcaatacctt cgtacggctg
     6541 ctcgccgtgc cggggatccg cgacacgcag tgcgggttca agctctttcg gggcgacgtc
     6601 gcgcgcgagc tcttttcgga ggcccgcatc gatcgattcg cctacgacgt cgagatcctc
     6661 tacctcgcgc gccgcgccgg gattccgatt gcggaggtcc cggtgctctg gttcaacagc
     6721 cccgagagca aggtcgcggt ggtcaaggac tcgctccgga tgctgcgtga cttgcttcga
     6781 atccgctgga tccaccgggg ccggaaaacg ggaaatagga aacgggaagc gaaagaggtc
     6841 cgttagagtc cttttccgtt tcccgtttcc cgtttctcga acaggggtat cagcctcgcc
     6901 gccacgcctg cagcgaccag cggggccagg gccgcggtcg tccggaagaa cgccgtctcg
     6961 accagccacc ccggaccggc gacagcgagc gacggcagga agaggtaggc ggagagtccg
     7021 cacgccgcga gagcgagcag gcgtcctccc gcgggcgacg ggcggcccgc ggcgaccaga
     7081 acggcaagcg cgacgagacc cgcccacgcg agctgcggcg tcagccggaa gactgctgtc
     7141 aggctctcac cgatccgcgg ggcgagctct cccggccggc ggaggagcga gaagtcgaaa
     7201 tcggagagcg gaaggctccc ccgaatcagc cggtgcgccg cgacgacgag cgcggcgggg
     7261 agggcaagaa tcgcggccgc ccgcgccacg actcctcgcc gggaaaacgc gaagagtgcc
     7321 gcgacgacga cgcccgcggc gacgaagaag aggccttcgt tcttcgtgcc gacgcaggcg
     7381 agcgaagcga gagcgagccg gcgcgacgcg ccgcggtccg cgcgctcca
//
LOCUS       DASWAV010000482         2821 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U2665
            k141_27139, whole genome shotgun sequence.
ACCESSION   DASWAV010000482 DASWAV010000000
VERSION     DASWAV010000482.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960316
            Sequence Read Archive: SRR10260007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2821)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2821)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:47:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,929
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2821
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_27139"
                     /isolate="SMAG_U2665"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.36444 N 132.31889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2501)
                     /locus_tag="VF958_10030"
     CDS             complement(<1..2501)
                     /locus_tag="VF958_10030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011600499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HEX9149487.1"
                     /translation="MTDPSRPTRLPPQSTDAGERMQQYPPPETWDDWVEWDQREWPRK
                     VPRRYMLVPTVCFNCESACGLLGWVDKETMQVRKFEGNPAHPGSRGRNCAKGPATINQ
                     VNDPDRILYPLKRAGRRGEGRWMRVSWEEALKDIGGRIRRALQEGRHNEVMYHVGRPG
                     HDGLMEWILPAWGLDGHNSHTNICSAGARAGYAFWMGFDRPSPDHANANVILLISSHL
                     ETGHYFNPHAQRIVEGQMAGAKLIVFDTRLSNTASRADTWISPWPGSEAAILLAIANH
                     LISTNRFDREFVRRWVNWEEYLRLVHPDLPVTFESFVQALKTIYAKYTFDFAARESGV
                     EARVLERIAEDVADCGGKLAAHNWRSAAAGNLGGWQVARCLWFLSVLTGSIGVEGGTS
                     ANAWDKWVPRPHKLAPHVKRWNELTWPGEYPLSYFEMSFLLPHFLKEGRGKLDVYFTR
                     VYNPMWTNPDGFSWLEVLRDESKIGLHVALTPTWSETAWWADYVLPMGHASERHDLMS
                     QETHAGQWIAFRQPVRRVALERLGRNVNRTYEANPGQVWEETEFWVDLSWEIDPDGSL
                     GIRQYFESPYRPGERITMDEYFGWMFENTVPGLPQAAAKEGLAPLAYMRRYGAFEIRA
                     GAEAEFERSLSSEEAATAHVDERTGILYTRAPATPPNNIVPTPAPATTKLGRPVGVMI
                     DGVPRLGFHTPSRKLEFYSPTVAEFGWPELAIPEYVRSHIHPESVDRAANEFVLLSTY
                     RLPTLIHTRSANAKWLVEISHSNPTWMHTSDAARLGLSNGDLVRVSTEIGWFVDKVWV
                     TEGIRPGVIACSHHLGRWRLAEGEGASRMASALVE"
     gene            complement(2509..>2821)
                     /locus_tag="VF958_10035"
     CDS             complement(2509..>2821)
                     /locus_tag="VF958_10035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="4Fe-4S ferredoxin"
                     /protein_id="HEX9149488.1"
                     /translation="EAARVLSWTLGASLLVDLLLTAGEFAVPHASAVAAAAAGLSTRG
                     RYAPHFWGSVVGGLALPLLLIVIAAGSPPAVALAGVLSLAGLFLYEWAFVMAPQQVPN
                     N"
BASE COUNT          452 a          931 c          978 g          460 t
ORIGIN      
        1 aactccacca gagcggaggc catgcggctg gccccttctc cttcggccag ccgccagcgg
       61 ccgaggtggt gcgagcaggc gatgacgccc gggcggatcc cttcggtgac ccagaccttg
      121 tccacgaacc agccgatctc cgtggagacg cgcaccaggt ccccgttcga gagccccagg
      181 cgcgccgcgt cggacgtgtg catccacgtc gggttggagt gggagatctc gaccagccac
      241 ttcgcgttcg cgctgcgggt gtggatcagg gtcggcagac gataggtcga gagcaggacg
      301 aactcattcg ctgcgcggtc gacggactcc ggatggatgt gactgcgaac gtactccggg
      361 atcgcgagct ccggccagcc gaactccgcg accgttgggg agtagaactc gagcttgcgt
      421 gacggagtgt ggaagccgag gcgcggcacc ccgtcgatca tcacgccgac tggacggccg
      481 agcttcgtgg tcgcgggcgc cggcgtcggg acgatgttat tcgggggcgt ggcgggagcg
      541 cgcgtgtaga gaatcccggt cctctcgtcc acgtgggccg tggccgcttc ctcgctcgaa
      601 agggagcgct cgaactcggc ctcggctccc gcgcggatct cgaacgcccc gtacctgcgc
      661 atgtaggcga gcggcgcgag accctctttc gccgcggcct gcggcaatcc cgggacggtg
      721 ttctcgaaca tccacccgaa gtactcgtcc atcgtgatgc gctcgccggg acggtacggt
      781 gactcgaagt actgacggat gccgagagaa ccgtcgggat cgatctccca ggagaggtcc
      841 acccagaact cggtctcctc ccacacctgg ccgggattgg cctcgtaagt gcggttgacg
      901 ttccgaccca ggcgctccag ggcgacgcgg cggaccggct ggcggaaagc aatccactgc
      961 ccggcgtgcg tctcctggct catcagatcg tgccgctcgg aagcgtgtcc catcggcaga
     1021 acgtagtcgg cccaccacgc ggtctcgctc cacgtcgggg tcagcgcgac gtggaggccg
     1081 atcttcgatt cgtcacgcag gacttcgagc cacgagaatc cgtccgggtt cgtccacatc
     1141 gggttgtaga cgcgggtgaa atagacgtcg agcttcccgc gcccctcctt caggaagtgc
     1201 gggaggagga agctcatctc gaagtaggag agcgggtact ctccgggcca ggtgagctcg
     1261 ttccatcgct tcacgtgcgg agcgagcttg tgcggccggg gcacccactt gtcccaggcg
     1321 ttggccgagg tgccgccttc gaccccgatc gaaccggtca ggacgctcag gaaccagaga
     1381 cagcgggcga cctgccagcc gccgaggttc cccgctgcgg cgctccgcca gttgtgcgcc
     1441 gcgagctttc cgccgcagtc ggccacgtct tcggcgatgc gctcgaggac gcgggcctcg
     1501 acccccgact cgcgggcggc gaagtcgaac gtgtatttcg cgtagatggt cttgagcgct
     1561 tgaacgaagg actcgaaggt caccggcagg tccgggtgca cgagacgcag gtactcctcc
     1621 cagttcaccc agcggcggac gaactcgcgg tcgaagcgat tcgtcgagat caggtggttg
     1681 gcgatcgcga gaaggatcgc ggcttccgag ccgggccacg gcgagatcca cgtgtccgcg
     1741 cgcgaggcgg tgttggagag gcgcgtgtcg aagacgatca gcttcgcccc ggccatctgg
     1801 ccctcgacga tgcgctgggc gtgcgggttg aagtagtgcc ccgtttccag gtggctgctg
     1861 atcaggagga tgacgttggc gttggcgtgg tcggggctcg gacggtcgaa gcccatccag
     1921 aacgcgtatc ccgcgcgtgc gccggcggaa cagatgttgg tgtgcgagtt gtgcccatcc
     1981 agaccccagg ccggcagaat ccactccatc aacccgtcgt gtccgggccg gccgacgtgg
     2041 tacatgacct cgttgtggcg gccctcctga agggcgcgcc ggatacgccc gccgatgtct
     2101 ttgagagctt cctcccagga gacccgcatc caccggccct ccccgcgacg gccggcgcgc
     2161 ttcaaggggt agaggatccg gtccgggtcg ttgacctggt tgatcgtggc cggccctttg
     2221 gcgcagttgc gcccccggct tccgggatgt gccgggttgc cttcgaactt gcgcacctgc
     2281 atcgtttcct tgtccaccca gccgaggagc ccgcacgccg actcgcagtt gaagcagacc
     2341 gtcggaacga gcatgtagcg gcgaggcact tttctcggcc actcccgctg gtcccattcg
     2401 acccagtcgt cccaggtctc ggggggcggg tactgctgca tccgttcgcc ggcgtcggtc
     2461 gactgcggcg gaaggcgcgt gggacgggaa ggatcggtca tctgccgctc agttgttcgg
     2521 gacctgctgc ggcgccatga cgaacgccca ctcgtagagg aacaggccgg cgagagagag
     2581 cactcccgcg agggcgaccg ccggaggaga tcccgccgca atcacgatca ggaggagtgg
     2641 cagagccagc ccgccgacca cgcttcccca gaagtgcggc gcgtagcgcc cgcgcgtgga
     2701 gagacccgcg gccgcggccg cgaccgcgct cgcgtgtggg accgcgaact ctcccgccgt
     2761 gagcaggaga tcgacgagca ggctcgcccc gagcgtccag gaaaggacgc gagccgcttc
     2821 c
//
LOCUS       DASWAV010000483         4069 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U2665
            k141_188015, whole genome shotgun sequence.
ACCESSION   DASWAV010000483 DASWAV010000000
VERSION     DASWAV010000483.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960316
            Sequence Read Archive: SRR10260007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 4069)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4069)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:47:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,929
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4069
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_188015"
                     /isolate="SMAG_U2665"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.36444 N 132.31889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..903)
                     /locus_tag="VF958_10040"
     CDS             complement(<1..903)
                     /locus_tag="VF958_10040"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HEX9149489.1"
                     /translation="MKQTEGVLDGRTLGRLDRWRYARALFVRPDGAEAERAVALAFGR
                     RWSRAQMYGRARRLLIESTPEAFLDRMQDTLGPLANDVRVAPEDPRQTKWDAMSNLAP
                     LSRAKSEVHALWFADLIRAGDFFAKFQPIADLERADILGYEGLLRAHSNSTSRPSAEM
                     FPAARVFRVERPFETLSWCSVLESAKVLPPDARLFLNVNPQLFGDTPEGLDALWRALD
                     EFAFAGSRLVLDLVEVENVASLDALARAVGEARQHGASVALDDVTSPYRTIQLCEALR
                     PEWVKVDCDITRGVARDVRRRPILK"
     gene            1015..1500
                     /locus_tag="VF958_10045"
     CDS             1015..1500
                     /locus_tag="VF958_10045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013967004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008199 - ferric iron binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HEX9149490.1"
                     /translation="MHESIDKKAVVEVLNHVLEAELAGVVRYTHYSFMVYGYTRIPIV
                     SWLREQATESIGHAQQAGEMITLLGEHPSLSIGKLLETHRHDVGDILREALDNERAGL
                     ALYQKLLGLVAGRDVMLEEYARRLITEESLHLAEVDKMLRRPGEVGTFAQPPGTRVKR
                     T"
     gene            1875..3209
                     /locus_tag="VF958_10050"
     CDS             1875..3209
                     /locus_tag="VF958_10050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011985971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEX9149491.1"
                     /translation="MSKKRNRGLTRRQFGKLTAAGALVVGGPAFLFPDRAKAAGKTLK
                     IIQWSHFVPGYDKWFDGVYTKEWGTKNDTEVIVDHVAIGEINARAAAEAAAKKGHDLF
                     MFLSPPAAYEKQVIDHREIYEEVQKKHGKMIPLAERSTYNPKTKKFFAFSDSYVPDPG
                     NYRKDLWQQVGFANGPDTWEDLRIGGKKIKDQFGNPVGVGLSQELDTNMAMRAVLWSF
                     GGAEQDEKGNVTLNSKRTIDAVKYVRALYKEAMTPEVFTWDPSANNRGILAGKLSFVC
                     NAISVTRTAEKDNPDMSSKIWVSPALKGPVRRIAAEHVMDCYVVWDFAENKEGAKKFL
                     ADYIDNFGAGFKASEFYNFPCFPQTVPDLKQQIATDAKANPPDKYKVLGDVLDWATNV
                     GYPGYASAAIDEVFNTFVLPTMFAKAARDEMSPEDAVTAADKEVRRIFDKWK"
     gene            3233..>4069
                     /locus_tag="VF958_10055"
     CDS             3233..>4069
                     /locus_tag="VF958_10055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019142556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX9149492.1"
                     /translation="MAVVSTRGLTKVFGKGQVGAVVDVNLESREGEFLVLLGPSGSGK
                     TTLLRMIAGLEEPTSGEIWIGGVLVNDWTPRQRRIAMVFQSYALYPHLTVLGNIEFPL
                     KARRMPKEERLKKVEWAAGLLGIGHLLKRKPRQLSGGERQRVALARAIVREPSLFLLD
                     EPLSNLDAKLRASAREELEQFQKKIGTTTIYVTHDQVEAMAMGDRVVVMNHGSVRQIG
                     SPADVYDEPADTFVATFLGSPPMNLIETPRVIAGFRPEHFYPANALPRDEPVLRLAFL
                     ISH"
BASE COUNT          796 a         1320 c         1289 g          664 t
ORIGIN      
        1 cttcaggatg gggcggcggc gcacgtcccg tgcaaccccc cgggtgatgt cgcaatccac
       61 tttcacccac tccggccgga gcgcctcgca gagctgaatc gtgcgatacg gagaggttac
      121 gtcatcgagc gcgacggaag cgccgtgctg ccgagcctct ccgacggcgc gggcgagggc
      181 atcgagcgac gcgacattct ccacctcgac gaggtcgagc acgagccgcg acccggcgaa
      241 ggcaaattca tcgagagcgc gccagagggc gtcgagccct tccggggtgt cgccgaagag
      301 ctgcgggttg acgttcaaga aaagtcgcgc gtcgggcggg agcactttcg ccgattcgag
      361 caccgagcac caggacagag tctcgaacgg gcgctcgacc cggaagacgc gcgccgccgg
      421 aaacatctcg gctgaaggcc ggctcgtcga gttcgagtgt gcgcgcagca atccctcgta
      481 cccgaggatg tcggcgcgct cgaggtctgc gatcggctgg aacttcgcga agaagtcgcc
      541 ggcgcgaatc agatccgcaa accagagcgc gtggacctcg ctcttcgcgc gggagagcgg
      601 cgcaaggttc gacatcgcgt cccacttcgt ctgtcgggga tcttccggag cgactcggac
      661 gtcgtttgcg agcggcccga gcgtgtcctg catccgatcg agaaatgcct cgggagtgct
      721 ttcgatgagc agacgcctcg ctcgcccgta catctgggca cgcgaccacc gccgaccgaa
      781 cgccagggcc accgcgcgtt ccgcctcggc accgtcagga cggacgaaga gggctcgcgc
      841 gtaccgccag cgatccagcc ggccgagggt ccggccgtcc agaacgccct cggtttgctt
      901 caacctggtc gtattttagc tctccccacg ccggggcacc gctttttctt cgtgacgcgg
      961 cgcacagata agattctccg gcccgaattg cggacatttg acaaggagga tctggtgcac
     1021 gagagcatcg acaagaaagc ggtcgtcgag gtcctgaacc acgtcctgga agctgagctc
     1081 gccggggtcg tgcgctacac gcattactcc ttcatggtct acgggtacac ccggatcccg
     1141 atcgtgtcct ggctccgcga gcaggcgacc gagtccatcg gacacgccca gcaggccggc
     1201 gagatgatca ctcttctcgg cgagcatccg tcgctctcga tcggaaaact gctcgagacc
     1261 catcgccacg acgtcggcga catcctccgc gaggcactcg acaacgagcg cgccggtctc
     1321 gcgctctacc agaaacttct gggcctcgtc gccgggcgtg acgtcatgct cgaggagtac
     1381 gcgcggcgcc tcatcaccga ggagtcgctg catctcgccg aagtcgacaa gatgctccgc
     1441 cggcccggtg aggtcgggac cttcgcgcag ccgcccggga cccgggtaaa gcgcacctag
     1501 caaggatgcc ggaatgcgcc cccggggcgt tgcgcgcggt ccgtcgcacc ggtgcgaacg
     1561 aatcaggtcg ggagcctcgt cgagagcgcg gcctcgtacc tccgtcgccg cgtttccgct
     1621 tgacatcccg tgaaactctg aaagactccg cgcgaacggc atgacgcggc gaaagccgga
     1681 tggtcagtct cgacagccca gccgattgct tctggcgatc tcgctctccc cgcgcccgtt
     1741 gccggaagtg agcaacgccg acaatgtccc tcaggcgaag agtcgggctc cctgaatcgg
     1801 ccgagcctcc aaccctccga caggcagagg ccccgcgcgc ggcgtgaatc gagtcgtcaa
     1861 acacggaggt gatcatgtcg aaaaaaagaa atcgcggact cacacggaga cagttcggca
     1921 agctgacggc cgccggcgct ctcgttgtcg gcggacccgc ctttctcttt cccgaccggg
     1981 caaaggcggc gggcaagacc ctgaagatca tccagtggag tcacttcgtc cccggttacg
     2041 acaagtggtt cgatggcgtc tataccaagg aatggggcac gaagaacgac accgaagtga
     2101 tcgtcgacca cgtcgcgatc ggggagatca atgcccgggc ggcggccgag gcggccgcca
     2161 agaaggggca cgacctcttc atgttcctct cccccccggc cgcttacgaa aagcaggtga
     2221 tcgaccatcg cgagatctac gaagaagtgc agaagaagca cggcaagatg atccctctcg
     2281 cggagcgctc gacctacaac ccgaaaacca agaagttctt cgccttctcg gactcgtacg
     2341 tccccgatcc gggaaactac cgcaaggacc tctggcaaca ggtcggcttc gccaacgggc
     2401 cggatacctg ggaggacctg cggatcggtg gcaagaagat caaggaccaa ttcgggaatc
     2461 ccgtgggggt cggtctctcc caggagctcg acacgaacat ggcgatgcgc gccgtgctct
     2521 ggtcattcgg aggcgcggag caggacgaga agggtaacgt cacgctcaac tccaagagga
     2581 cgatcgacgc cgtcaagtac gtgcgcgcgc tctacaagga ggcgatgacc ccggaggttt
     2641 tcacctggga tccttccgcg aacaatcgtg gaatcctggc cggaaagctc tctttcgtat
     2701 gcaacgcgat ctcggtgacg cggaccgccg agaaggacaa tcccgacatg tcctccaaga
     2761 tctgggtttc tccggcgctg aagggtcccg tgcggcggat cgccgccgag cacgtcatgg
     2821 actgctacgt cgtctgggat ttcgccgaga acaaggaggg cgccaagaag ttcctggccg
     2881 actacatcga caacttcggc gcgggcttca aagcgagcga gttctacaac tttccgtgct
     2941 tccctcagac cgttcccgat ctgaagcaac agatcgcgac cgacgccaaa gccaatccgc
     3001 ccgacaagta caaggtgctc ggagacgtcc tcgactgggc gacgaacgtc gggtacccgg
     3061 ggtacgcgag cgcggccatc gacgaggtct tcaacacctt cgtgctgccg acgatgttcg
     3121 caaaggccgc gcgagacgag atgagcccgg aggacgcggt gacggcggcg gacaaggagg
     3181 tccggcggat cttcgacaag tggaagtaga cccgtcatga cgcgcccgtg atatggccgt
     3241 cgtttccacg cgaggtctca ccaaagtctt cggcaaggga caggtcggag cggtcgtcga
     3301 tgtgaatctg gagagccggg agggcgagtt tctcgtcctc ctcggccctt cggggtccgg
     3361 gaaaacgacg cttctccgga tgatcgccgg cctggaggag ccgacctccg gcgagatctg
     3421 gatcgggggt gttctcgtca acgactggac gccccggcag cggcggatcg ccatggtctt
     3481 ccagagctat gcgctctatc cccatctgac cgtgctcggc aacatcgagt ttccgctgaa
     3541 ggcgcggcgc atgccgaagg aggagcgcct caagaaggtg gagtgggcgg cgggactcct
     3601 cggtatcggg cacctcttga agcgcaagcc gcgtcaactc tccggaggag agcggcagcg
     3661 cgtcgcgctc gcccgtgcaa tcgtgcggga gccatcactc ttccttctcg acgagcctct
     3721 ttcgaatctg gacgccaagc tccgagcctc ggcccgtgag gagctcgagc agttccagaa
     3781 gaagatcgga accaccacga tctacgtcac acacgaccag gtcgaggcga tggcaatggg
     3841 cgaccgagtc gtcgtgatga atcacggttc cgtccggcaa atcggtagcc ccgcagacgt
     3901 ctacgacgag ccggcggaca ctttcgtggc aacgttcctc ggctcccctc ccatgaatct
     3961 gatcgagacc ccgcgcgtga tcgccggctt tcgacccgag cacttctacc cggcgaacgc
     4021 gctgccgcga gatgagccgg tcctgcgact cgcgttcctc atcagccac
//
LOCUS       DASWAV010000484         7115 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U2665
            k141_467220, whole genome shotgun sequence.
ACCESSION   DASWAV010000484 DASWAV010000000
VERSION     DASWAV010000484.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960316
            Sequence Read Archive: SRR10260007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 7115)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7115)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:47:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,929
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7115
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_467220"
                     /isolate="SMAG_U2665"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.36444 N 132.31889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..838)
                     /gene="coaBC"
                     /locus_tag="VF958_10060"
     CDS             complement(<1..838)
                     /gene="coaBC"
                     /locus_tag="VF958_10060"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR00521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004632 - phosphopantothenate--cysteine
                     ligase activity [Evidence IEA];
                     GO_function: GO:0004633 - phosphopantothenoylcysteine
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0015939 - pantothenate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HEX9149493.1"
                     /translation="MNPSPHVLLGVSGGIAAYKSAEIVRRLVDRGADVRVTMTRAARE
                     FVAPLTFAVLSKHEVYLEIFGGGNVPAVDHVELAEWADVLVVAPATAHTIARLANGLA
                     DDFLTTYALVHRGPVLVAPAMETRMWEHPATRRNLELLATRGARFVGPGSGFLASGRE
                     GAGRMAEPEEIAEAAMALATSARRDLEGLRVLVSAGPTRERLDPVRYVSNRSSGRMGY
                     ALAEAARDRGARVTLVSGPTGLVRPEGMRFVSFETAAELDNLLRAEFPECDVLAMAAA
                     VAD"
     gene            complement(867..1136)
                     /gene="rpoZ"
                     /locus_tag="VF958_10065"
     CDS             complement(867..1136)
                     /gene="rpoZ"
                     /locus_tag="VF958_10065"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HEX9149494.1"
                     /translation="MIQTIPDGVDSKFRLVLLIARRAEQLMRGARPKISLERPLKPTR
                     LAAEEFEDNQIRWDYGEEGGVLEPSGEESGGEPGVEEEEPVSSLD"
     gene            complement(1149..1787)
                     /gene="gmk"
                     /locus_tag="VF958_10070"
     CDS             complement(1149..1787)
                     /gene="gmk"
                     /locus_tag="VF958_10070"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018718675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004385 - guanylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HEX9149495.1"
                     /translation="MTRQPRGNLFIVSSPSGGGKTTLIRRLIADLPGAPLHFSVSHTT
                     RPRRTGEKNGREYHFVTDSEFRRMAARGGFLEHNRVHGRRYGTSRAEVLPRLARGEDV
                     VLDVDVQGASDLRKAYPKAVSVFIVPPDRGELERRLRARGLDRGVDEEKEIRKRLRTA
                     RAEIRKAKEFQYVIVNDDLERATLELQSIVRATRVTHARQKTRLERLFREFR"
     gene            complement(1784..2671)
                     /locus_tag="VF958_10075"
     CDS             complement(1784..2671)
                     /locus_tag="VF958_10075"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YicC/YloC family endoribonuclease"
                     /protein_id="HEX9149496.1"
                     /translation="MIRSMTGFGRAKGNVGEDWTAEVVARSVNHRFLDVNVKLREQEI
                     DLEPVLRRAFSRHIARGKVEVSLRLRRATPAPTVISIDEGLLEALLSRFAALSERYPV
                     SGRLEARDLLTIPQLFSVESTVDGFTETHVAALEEIAEQAARALVGMREAEGALVAAD
                     LAERILFLEKRLQQLSERRGEIVRRALDTLRERLRVLLSEPGLDPGRLEQEAALAADR
                     SDVAEELQRLEGHLQQFGSLLASAREPVGKKLDFLAQEILRELNTLGSKGRDLQIIRE
                     VLEMKSEIEKIREQVQNLE"
     gene            complement(2668..4527)
                     /locus_tag="VF958_10080"
     CDS             complement(2668..4527)
                     /locus_tag="VF958_10080"
                     /inference="COORDINATES: protein motif:HMM:NF012869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX9149497.1"
                     /translation="MSPLRRLLEYFRPYRGRAAAALLAMGIVSLSTVLLLFLLTKIID
                     DVLGAGTASSIRGFGAAPAKTAVFVRWLDSIYSGARAAAEASGIPIRFAVPILLLAAL
                     VSKNVFSYFSEFAFNGIGLSMVRDLRRDAYGQLLGQSAAFYSRSTTGDLLARLLSDVE
                     LIQSAFGTRLADFVQGAVTILLVLFYVVSLNAQLSLFVLLLAPVLLLPIIEVTRRLRR
                     TTFSSRERIGDMGALLSETLRGHRVIKTYGMEPFEAERFERANDRYFRVTRRTVRIQA
                     LNSPMMEVIAGVGLSAIFVYAATQIRAGRMTVGGLVSFLAALMMMYKPLKDVTKVNMA
                     LQLALSSARRLFELIDSKSEIVEKPDAVELPPFSRAIRYENVSFRYGEDEAVLQDVDV
                     TIRRGETVAIVGPSGAGKTTLVNLLPRLYDPTAGKITFDGIDLKDVTLASLRRQIGIV
                     TQDTVLFDTTARDNIAYGRAQEPEEGVRAAAKAAFADEFVSRLPAGYDTRLGEDAGRL
                     SGGQKQRLAIARALYKDAPILILDEATSQLDSESEALVARALSNLMARRTTLVIAHRL
                     STVRRADRILVLDGGRVVESGTHQELLTRNGLYRRLHDMQYFVETEISAEARR"
     gene            complement(4524..5657)
                     /gene="lpxB"
                     /locus_tag="VF958_10085"
     CDS             complement(4524..5657)
                     /gene="lpxB"
                     /locus_tag="VF958_10085"
                     /EC_number="2.4.1.182"
                     /inference="COORDINATES: protein motif:HMM:TIGR00215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008915 - lipid-A-disaccharide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-A-disaccharide synthase"
                     /protein_id="HEX9149498.1"
                     /translation="MNLLISAGEASGDLHGARLLEALKRRRPDLSAFGMGGERLERAG
                     LQRIVRSENLSVVGVFEVFEKLPALWKGLAKMRSASRRLAPDAAVLIDFPDFNGILAR
                     RLHRDGVPIVYYVSPQVWAWRAGRARSIARLARRIVTLFPFEAEIYRRLGADAVCAGH
                     PLVDSVREALESEAPSPHRKGHRLVLLPGSRVAEVRRHWVPMLDAAQALARRFDLDVA
                     VVRAPGLPERLFPGASELGFPLIDTGRHALIASADLAFVASGTATLETALCGTPMVVV
                     YRTSAASFAIGKRLVKVPWISLVNIVAGEGIVPELIQEQFHAARLEKEGAALLGSPEE
                     LAKMRFSLARVARQLGPPGGSERAADAVLEVLEPEERRRSAGP"
     gene            complement(5731..5922)
                     /locus_tag="VF958_10090"
     CDS             complement(5731..5922)
                     /locus_tag="VF958_10090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9149499.1"
                     /translation="MRFKILPADHFLIMAVYAILVSAFFGLLWRDERQDRLRLFGILL
                     AALVLGGLAVAWLMYPFPS"
     gene            complement(5919..6308)
                     /locus_tag="VF958_10095"
     CDS             complement(5919..6308)
                     /locus_tag="VF958_10095"
                     /inference="COORDINATES: protein motif:HMM:NF042755.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6677 family protein"
                     /protein_id="HEX9149500.1"
                     /translation="MSAVAVRRRVHPAVAALLAWIFPGLGHAYLGRRKTAVAYAAIVT
                     ATFLLGLSFEGRLYTIDRSQPLTILATFAVSGAGLLNVAARFLTENPGGTILASTYEY
                     GCAYLLTAGLMNLLLMLDAWDVAAGNK"
     gene            complement(6305..6796)
                     /locus_tag="VF958_10100"
     CDS             complement(6305..6796)
                     /locus_tag="VF958_10100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011686016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIT domain-containing protein"
                     /protein_id="HEX9149501.1"
                     /translation="MPEVLFAPWRYEYLVAEKSETCIFCEAAASGEGEESLVVFRGRH
                     VFVLLNRYPYTNGHLMVAPYGHEARLSDSNEETLKELVKVIARGEKILGETYRTDGLN
                     IGVNLGSAAGAGISGHYHVHLVPRWNGDTNFMTVAGNVRVVPEELPATRRRLAPLFEK
                     VGR"
     gene            complement(6828..>7115)
                     /locus_tag="VF958_10105"
     CDS             complement(6828..>7115)
                     /locus_tag="VF958_10105"
                     /inference="COORDINATES: protein motif:HMM:NF012442.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HEX9149502.1"
                     /translation="EGVAMTKAELIERVAVATDVTKKQAEGIVDTIFEAIIRSLKQGQ
                     KIELRGFGSFRLRERGARIGRNPKTGVKVSVPAKKIPYFKPGKELKELINR"
BASE COUNT         1194 a         2377 c         2436 g         1108 t
ORIGIN      
        1 agtcggcgac cgccgccgcc atcgcgagga cgtcgcactc ggggaactcc gcacgcagca
       61 ggttgtcgag ctctgcggcc gtctcgaagg aaacgaaccg catcccctcc ggacgaacga
      121 gaccggtcgg gccggagacg agggtcaccc ttgcaccccg gtcgcgcgcc gcttcggcga
      181 gcgcgtaccc catccggccg ctcgaacggt tcgacacgta gcggacggga tcgagccgct
      241 cgcgggtcgg accggcagag acaaggacgc ggagcccttc gaggtcccgt cgcgcggagg
      301 tcgcgagcgc catcgccgcc tcggcgatct cctcgggctc ggccatgcga cccgcgccct
      361 cccggccgga cgcgaggaat ccgctgccgg gtccgacgaa acgggcgccg cgcgtcgcca
      421 gcagctcgag gttgcgccgt gtggcggggt gttcccacat gcgcgtctcc atcgccggcg
      481 cgacgagaac cggcccgcgg tggacgagag cgtacgtcgt caggaaatcg tccgcgagcc
      541 cgtttgcgag ccgggcgatc gtgtgtgcgg tcgccggagc caccacgagg acgtcggccc
      601 attcggcgag ctcgacgtga tcgacggcgg ggacgttgcc gccgccgaag atctcgaggt
      661 agacctcgtg cttcgagagg accgcgaacg tgagtggcgc gacgaattcg cgggccgcac
      721 gcgtcatcgt gacgcgaacg tccgcgccgc ggtcgacgag ccgcctcacg atctcggcgg
      781 acttgtaggc ggcgatgccg ccggacacgc cgaggaggac gtgcggactc gggttcatcg
      841 gccgatcggg cgcgcctcgc gcctcgtcag tcgagcgagg aaaccggctc ttcttcctcg
      901 acgcccggct ctccgccgct ctcttcgccg gagggttcga ggactccgcc ctcttcgccg
      961 tagtcccagc gaatctggtt gtcttcgaac tcctcggccg cgaggcgtgt cggcttcaaa
     1021 ggccgctcga gcgagatctt cggccgagcg cccctcatga gctgctcagc tcgccgcgcg
     1081 atcagaagga cgagcctgaa cttgctgtcc accccgtcgg gaatggtctg gatcattcga
     1141 aacctctttc agcggaattc gcggaagagg cgctcgaggc gcgtcttctg ccgggcgtgg
     1201 gtgacccgag tggcccgaac gatggattgt agctcaagtg tggcccgttc caggtcgtcg
     1261 ttgacgataa cgtactgaaa ctctttagct ttccggattt ccgcccgagc ggtccgaagc
     1321 ctcttccgga tctccttttc ctcgtccact ccccggtcga gcccccgcgc tcgcagacgg
     1381 cgctcgagct ctccgcggtc gggagggacg atgaagaccg agaccgcctt cgggtacgcc
     1441 ttgcgcaggt cggaagcacc ctgaacgtcg acgtccagca cgacgtcttc ccccctcgcc
     1501 agccgcggaa ggacctccgc gcgtgacgtg ccgtagcggc gcccgtggac gcggttgtgc
     1561 tcgaggaaac caccccgcgc ggccatcctc cgaaactcgg agtcggtgac gaagtggtac
     1621 tcgcggccgt tcttctcccc cgtgcgtcgc ggccgcgtcg tgtgcgagac ggaaaagtgc
     1681 agcggagcgc cggggagatc cgcgatgaga cgccggatga gcgtcgtctt gcctcctccg
     1741 gagggcgacg acacgatgaa gaggttgcct cggggctggc gcgtcattcc aggttctgca
     1801 cctgctcgcg gatcttctcg atctcggatt tcatctcgag gacctcgcgg atgatctgga
     1861 ggtcgcgccc cttggacccg agcgtgttca gttctcgcag gatctcctgc gcgagaaagt
     1921 cgagcttctt gccgaccggc tcgcgcgcgg aggcgaggag cgaaccgaac tgctggaggt
     1981 ggccctcgag acgctggagc tcctccgcga cgtccgagcg gtccgcggca agcgcagcct
     2041 cctgctcgag gcgcccggga tcgagtcccg gctccgacag gagcacgcgg agacgctccc
     2101 tcagggtgtc gagcgcacgg cgcacgatct ccccgcggcg ctccgaaagc tgctgcagcc
     2161 gcttctcgag aaagaggatg cgttcagcga ggtcggcggc gacgagggca ccttctgcct
     2221 cccgcatccc gacgagcgca cgcgccgcct gctcggcgat ttcctcgagc gctgcgacgt
     2281 gggtctccgt gaagccgtcg accgtgctct cgaccgagaa gagttgcgga atcgtgagca
     2341 ggtcgcgggc ctccaggcgg ccgctcaccg ggtatctctc cgagagggcg gcaaaccgcg
     2401 agagcaacgc ctcgaggagc ccctcgtcga tcgagatcac cgtcggcgcg ggcgtcgcac
     2461 gccgcagtcg cagggaaact tcgaccttgc cgcgggcgat gtgccgcgag aaggcgcgcc
     2521 gcaataccgg ctcgaggtcg atctcctgct cccggagctt cacgttcacg tcgaggaacc
     2581 ggtggttgac gctgcgcgcg acgacctccg cggtccagtc ctccccgacg ttccccttcg
     2641 cgcggccgaa gccggtcatg cttcggatca ccggcgcgcc tccgcggaga tctcggtctc
     2701 gacgaagtac tgcatgtcgt gcagcctgcg gtagagcccg ttgcgagtca acagctcctg
     2761 gtgggttccc gactccacca cgcgcccacc gtcgagtacc aggatccggt cggcccggcg
     2821 caccgtcgag agccggtgtg cgatcacgag cgtcgtcctt ctcgccatca ggttcgacag
     2881 ggcgcgcgcg acgagggcct cggactccga gtcgagctgc gaggtcgcct cgtcgaggat
     2941 caggatcggc gcgtccttgt agagggcccg cgcgatcgcg agacgctgct tctgaccgcc
     3001 ggaaaggcgt cccgcatcct cgccgagacg ggtgtcgtag cccgcgggaa gacgagacac
     3061 gaactcgtcg gcgaaagccg ctttcgcggc ggcgcggacc ccttcctccg gctcctgcgc
     3121 ccggccgtag gcgatgttgt cgcgtgcggt cgtatcgaag agaacggtgt cctgggtgac
     3181 gatcccgatc tgacgccgaa gagacgcgag agtgacgtcc ttgagatcga ttccgtcgaa
     3241 cgtgatcttt cccgcggtcg ggtcgtagag ccgcggcagc aggttgacga gcgtcgtctt
     3301 tccggcgccc gagggtccga cgatcgcgac cgtctcgccg cggcggatcg tgacgtccac
     3361 gtcctgcagg accgcttcgt cctccccgta gcgaaaggag acgttctcgt agcggatcgc
     3421 gcgggagaac ggcggcagct cgacggcgtc gggcttttcc acgatctcgc tcttcgagtc
     3481 gatgagttcg aagaggcggc gcgccgagga gagggcgagc tgcagcgcca tgttgacctt
     3541 cgtcacgtcc ttcagcggct tgtacatcat catgagggcc gcgaggaagg acacgaggcc
     3601 tccgacggtc atccgtcccg cgcggatctg agtcgccgca taaacgaaga ttgcggaaag
     3661 gcccacgccc gcgatgacct ccatcatcgg cgaattgagc gcctggatgc gcaccgtccg
     3721 ccgcgtgacc cggaagtacc ggtcgttggc gcgctcgaaa cgttccgcct cgaagggctc
     3781 cattccgtag gtcttgatga cgcgatgacc gcgcagcgtc tcggagagga gcgcgcccat
     3841 gtcgccgatc cgctcgcggg aggagaacgt cgtgcgtcgc aggcgccgcg tgacctcgat
     3901 gatggggagc agaagcacgg gggcgagcag gagcacgaag agcgaaagct gagcgttcag
     3961 ggacacgacg tagaagagga cgagcaggat cgtcaccgct ccctggacga agtcggccag
     4021 gcgcgtgccg aaggccgatt ggatgagctc gacgtcggaa agaagccggg cgaggaggtc
     4081 gcccgtcgtc gagcgcgagt agaaggccgc ggactgcccg agaagctgtc cgtacgcgtc
     4141 gcgacgcagg tcccggacca tcgagagacc gatgccgttg aacgcgaact ccgagaagta
     4201 ggagaagacg ttcttcgaga cgagcgcagc gaggagcagt atcggaacgg cgaaccggat
     4261 cgggataccc gacgcctccg cggccgcccg ggcgcccgag tagatcgagt cgagccaccg
     4321 cacgaagacc gccgtcttcg cgggggcggc tccgaagccg cgaatcgacg acgccgtgcc
     4381 ggcgcccagc acgtcgtcga tgatcttcgt gaggagaaag agtaggagga cggtcgagag
     4441 agacacgatc cccatcgcaa ggagcgccgc ggcggcacgg ccgcggtacg ggcggaagta
     4501 ctcgaggagc cgtcggaggg gactcatggt ccggcggaac gccgccgctc ctcgggttcc
     4561 aggacctcca ggacggcgtc ggctgcgcgc tcgctccccc cgggcggacc gagctgccgc
     4621 gcgacgcgcg ccagcgagaa acgcattttc gcgagctctt ccggggagcc gagaagcgcc
     4681 gcgccctctt tctcgaggcg cgccgcgtgg aactgctcct ggatgagctc gggcacgatt
     4741 ccttccccgg ccacgatgtt cacgagcgag atccacggca ctttgaccag tcgttttccg
     4801 atcgcgaagc tcgcagccga cgttcgatag acgacgacca tcggagtccc gcagagggcg
     4861 gtctccagtg tcgccgttcc cgatgccacg aacgcgaggt ccgccgacgc gatgagcgcg
     4921 tgccgcccgg tgtcgatcag ggggaagcca agctccgaag cgcctggaaa cagcctctcc
     4981 ggcaggccgg gcgcgcgcac gacggcgacg tcgaggtcga agcggcgcgc gagggcctgc
     5041 gccgcatcga gcatcggtac ccagtggcgg cgcacttccg cgacccggct ccccgggagg
     5101 agaacgaggc ggtgcccctt cctgtgcggg gacggggctt ccgattcgag cgcttcgcgg
     5161 acggagtcga ccagcgggtg cccggcgcag acggcatccg cgccgaggcg ccggtagatc
     5221 tccgcctcga aggggaagag cgtcacgatg cgccgcgcca atctcgcgat cgaacgggcg
     5281 cgcccggccc tccacgccca cacctgggga ctcacgtagt acacgatcgg gacgccatcg
     5341 cggtggagcc ggcgcgcgag aatcccgttg aagtcgggaa agtcgatcag gaccgcagcg
     5401 tcgggagcga gccgccgcga ggcgctccgc attttcgcga ggcccttcca gagagcgggc
     5461 agcttctcga agacctcgaa gactccgaca acggagaggt tttcggaccg cacgatccgc
     5521 tggagtcccg cccgctccag ccgctctccc cccattccga acgcggagag gtcggggcgg
     5581 cggcgcttga gcgcttcgag aagacgcgcc ccgtggaggt ctccggaggc ctcgccggcg
     5641 gagatgagaa ggttcattct tctcttgtca ttctgaggcc cgaaaggccg aagaatctcc
     5701 ccctttttcg gaggggatcc ttcgcttcgc tcaggacgga aacgggtaca tcagccacgc
     5761 gacggcgagg ccgcccagca cgagcgccgc gagaaggatc ccgaagaggc gcagccgatc
     5821 ctgcctctcg tcgcgccaga ggagcccgaa gaaggccgag acgaggatgg cgtaaacggc
     5881 catgatgaga aagtggtccg cgggaaggat cttgaacctc acttgttccc cgcggcgacg
     5941 tcccacgcgt cgagcatcag gagaaggttc atgaggccgg cggtgagcag gtaggcgcaa
     6001 ccgtactcgt acgtcgacgc caggatcgtc ccgcccgggt tctcagtcag gaatcgcgcc
     6061 gcgacgttca ggagtcccgc gcccgacacg gcgaaagtcg ccaggatcgt gagcggctgc
     6121 gatcggtcga tcgtgtacag ccgtccctcg aaggacagcc cgaggagaaa cgtcgccgtg
     6181 acgatcgccg cgtacgcgac cgccgtcttg cggcggccga ggtacgcgtg gcccagcccc
     6241 ggaaagatcc acgcgaggag cgcggcgacc gccggatgga cgcggcgccg gaccgcgacg
     6301 gcgctcaacg gccgaccttc tcgaacagcg gtgccaggcg ccggcgcgtg gccgggagct
     6361 cttcgggcac gacgcgcacg ttgcccgcca ccgtcatgaa gttcgtgtct ccgttccagc
     6421 ggggcacgag atgaacgtgg tagtggccgg agattccagc tcccgcggcg gacccgaggt
     6481 tgacgccgat gttcaggccg tcggtgcggt aagtctcgcc gaggatcttc tctccccggg
     6541 cgatgacctt gacgagctcc ttcagggtct cctcgttcga gtcggagagc cgcgcctcgt
     6601 ggccgtaggg cgcgaccatc aggtgaccgt tcgtatacgg ataccgattc aggagcacga
     6661 agacgtgccg cccgcgaaag acgacgaggg attcttcccc ctctcccgag gcggcggcct
     6721 cacagaagat gcacgtttcg gacttctcgg ccacgaggta ttcgtaacgc cacggggcga
     6781 agaggacttc cggcattgtg tgctctcggc gatcgacaaa ccgggagcta acgattgatg
     6841 agctccttca gctccttgcc cggtttgaag taggggatct tcttcgcggg gacggagacc
     6901 ttgacgccgg tttttggatt ccggccgatc cgcgcgccgc gctctcggag gcggaacgag
     6961 ccgaagccgc gcagctcgat cttctgcccc tgcttgaggg accggatgat cgcctcgaag
     7021 atcgtatcca cgatcccctc ggcctgcttc ttcgtgacgt ccgtcgcgac cgccacgcgc
     7081 tcgatcagtt cggccttcgt catcgcgact ccctc
//
LOCUS       DASWAV010000485         4459 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U2665
            k141_428382, whole genome shotgun sequence.
ACCESSION   DASWAV010000485 DASWAV010000000
VERSION     DASWAV010000485.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960316
            Sequence Read Archive: SRR10260007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 4459)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4459)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:47:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,929
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4459
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_428382"
                     /isolate="SMAG_U2665"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.36444 N 132.31889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1954
                     /gene="speA"
                     /locus_tag="VF958_10110"
     CDS             <1..1954
                     /gene="speA"
                     /locus_tag="VF958_10110"
                     /EC_number="4.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014100556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008792 - arginine decarboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="biosynthetic arginine decarboxylase"
                     /protein_id="HEX9149503.1"
                     /translation="MAAPKPIRRPHSAPPIARSRPGERFDDSAVLYGIPHWGKRFFHV
                     SEDGNLVVRPTREPERGVALKQIVDEVGLRGITPPLIIRFPQILASAVSDLNNAFARA
                     IEEFEYGNVYRGVFPIKVNQKRVVVEQIIEAGRPYGYGLEAGSKPELLAAVAADLAPD
                     SLIVCNGYKDEAFIGMALNGIRMHKKVVLVLEKVSELDRILTVARQRHVRPLLGMRVK
                     LYARGSGKWAKSGGEAAKFGLTTPEMLETVKILRRRRMLDTMVMLHFHIGSQITDIRK
                     IKDAIREAGRVYAKLRALGVPIKYLNLGGGLGVDYDGSKTAFDSSMNYSVQEYANDVV
                     YTIKSICDEEKVPEPTLVTESGRAITAFHSVLVTNVLDVADRIEQGRRVRVSPSDPNV
                     IQELSGILKEVNAKNLRESYHDALQYKEELFTLFNLGHLSLEDRSKGEMLFWQICERI
                     HRDLKTLKEIPEEFEDMETMLADKYVLNFSVFQSLPDSWAIDQLFPILPIHRLNEKPT
                     EFATLADITCDSDGKIERFIDLRDIDETLPLHPFRHDKPYYIGVCLMGAYQDVLGDLH
                     NLFGEAHEVLVRVDQEGRSEIEDVLPGETCERVLEYMNYDRTHLLDSINEQLVRAASS
                     GMKKSEVQSIYREFEAVFPRYTYLER"
     gene            1979..2725
                     /locus_tag="VF958_10115"
     CDS             1979..2725
                     /locus_tag="VF958_10115"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018626394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HEX9149504.1"
                     /translation="MLPIGDDDTGRTITPIVNYLLIAANVAVFVFLQGLGSNERFTYA
                     FSTVPQEIVTGKDVAQPVTIKDPVSGEVAGTVELEPTPIPVYLTLLTSMFMHGGIAHI
                     FGNMLYLWIFGDNLEDSMGHLRYLIFYLVCGIIAGLSHVFATTALGGNPYLPALGASG
                     AISAVLAGYLVLHPKRRVRVIMLRTIQEVPAIWAIGIWFVFQLVSGLGMLGGGSQAGG
                     VAYAAHIGGFIAGLVLVKVFAAGRPRYQYT"
     gene            2722..3123
                     /locus_tag="VF958_10120"
     CDS             2722..3123
                     /locus_tag="VF958_10120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020144453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HEX9149505.1"
                     /translation="MTRRNVFSGAPWEPVVGYSRAVRVGNMVWVSGTTATDPSGRIAA
                     AGDAYAQAVQALANIRTALEKAGASLADVVRTRMYVVNVADWQEVGRAHAEFFGDVRP
                     ASTLVEVSALVSPEMLVEIEAEAVVGGGVST"
     gene            complement(3113..>4459)
                     /locus_tag="VF958_10125"
     CDS             complement(3113..>4459)
                     /locus_tag="VF958_10125"
                     /inference="COORDINATES: protein motif:HMM:NF018743.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033922 - peptidoglycan
                     beta-N-acetylmuramidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exo-beta-N-acetylmuramidase NamZ
                     domain-containing protein"
                     /protein_id="HEX9149506.1"
                     /translation="FGHTGFTGTSLWIDPSSGVFVVFLSSRLHPDGKGDVNRLRGLVA
                     TIVASALTEDTRKPARQLAERAPIHREVLAGVDVLEAEGFRAIAGKRVGLVTNATGRA
                     RDGRSTVEVLVSEKAKKAGVKLVALFSPEHGIRSDADAPVADQVDPTTGLTIHSLYGE
                     RRRPTPGELEGLDALLLDVQDVGTRFYTYITTGGYLLPEVARAKIPLVVLDRPDPISG
                     EAIEGPVSDPDRLSFTAHHTIPVRYGMTPGELLTMVNDEQKAGARVEVVKLSGWSRDL
                     WFDETGLEWVNPSPNMRSLTEATLYPGIGLLETTNLSIGRGTDTPFEVIGAPWLDGRR
                     LAAVAAARKIPGVTFTPIHFTPASSTFGGERCGGVRLTVTDRDALRPVALGIEIAVAL
                     RDLYPVDWQRAKLISLLANRDTFERVERGETADAIARSWEEGLEEFRRRRAQFLRY"
BASE COUNT          763 a         1497 c         1455 g          744 t
ORIGIN      
        1 gatggccgcg ccaaagccga ttcgccgccc gcattccgcg ccgccgatcg cccgctcgcg
       61 cccgggagag cggttcgacg actctgccgt gctctacggc atcccgcact ggggcaagcg
      121 tttcttccac gtctcggagg acggcaacct ggtcgtccgc ccgacccggg agcccgagcg
      181 gggcgtcgcc ttgaagcaga tcgtcgacga ggtcggcctg cgcgggatca cgccgccgct
      241 gatcatccga ttcccgcaga tcctcgcctc cgccgtgtcc gacctgaaca acgcgttcgc
      301 gcgcgcgatc gaggagttcg agtacggcaa cgtctaccgg ggggtctttc cgatcaaggt
      361 gaatcagaag agggtcgtcg tcgaacagat catcgaggcc gggcgaccgt acgggtacgg
      421 actcgaggcg ggctccaagc ccgagcttct cgccgcggtc gccgcggatc tcgcgcccga
      481 ctcgctcatc gtctgcaacg gatacaagga cgaggcgttc atcggaatgg cgctgaacgg
      541 catccggatg cataagaagg tcgtgctcgt cctcgagaag gtgtcggagc tcgaccggat
      601 cctgacggtc gcgagacagc gtcacgtgcg gccgctgctc ggcatgcgcg tgaagctgta
      661 cgcgcggggc tccggaaagt gggcgaagtc cgggggagaa gcggcgaagt tcggcctgac
      721 gacccccgag atgctcgaga ccgtcaagat cctgaggcgc cgccggatgc tcgacacgat
      781 ggtcatgctc cacttccaca tcggctcgca aatcacggac atccgcaaga tcaaggacgc
      841 gatccgggaa gcgggccgcg tgtacgcgaa gctgcgggcg ctcggcgtcc ccatcaagta
      901 cctgaacctc ggcggaggtc tcggcgtgga ctacgacggt tcgaagaccg cgttcgactc
      961 ctcgatgaac tactcggtcc aggaatacgc gaacgacgtc gtctacacga tcaagtccat
     1021 ctgcgacgag gagaaggtgc ccgagcccac gctggtcacc gagtccgggc gcgcgatcac
     1081 ggcgttccac tcggtcctcg tcaccaacgt tctcgacgtc gcggatcgca tcgagcaggg
     1141 gcggcgcgtc cgcgtttcgc cgagcgaccc gaacgtcatc caggagctct cgggaatcct
     1201 gaaagaggtc aacgcgaaga accttcggga gagctaccac gacgccctcc agtacaagga
     1261 agagctcttc acgttattca accttgggca cctgtcgctc gaggatcgct cgaagggcga
     1321 gatgctgttc tggcagatct gcgagcggat ccaccgggat ctgaagaccc tcaaggagat
     1381 tcccgaggag ttcgaggaca tggagacgat gctcgcggac aagtacgtcc tgaacttctc
     1441 cgtcttccag tctctgcccg actcgtgggc gatcgatcaa ctcttcccga tcctcccgat
     1501 ccaccgtctg aacgagaagc cgaccgagtt cgcgacgctg gcggacatca cctgcgactc
     1561 cgacgggaag atcgagcgtt tcatcgacct gcgggacatc gacgagaccc tgccgctcca
     1621 cccgttccgc cacgacaagc cctactacat cggcgtctgc ctgatgggcg cctatcagga
     1681 cgtcctgggc gaccttcaca acctcttcgg cgaagcgcac gaggtcctcg tgcgggtgga
     1741 ccaggagggg cggtccgaga tcgaggacgt cctgccggga gagacgtgcg agcgcgtgct
     1801 cgaatacatg aactacgacc ggacgcacct gctcgactcg atcaacgagc agctcgtgcg
     1861 cgccgcctcc tccgggatga agaagagcga agtccagtcg atctaccggg agttcgaagc
     1921 ggtctttccc aggtacacct acctcgaacg atagcagtca gcggacggag gaggtcggat
     1981 gcttcccatc ggcgacgacg acaccggacg cacgatcacc ccgatcgtca attacctgct
     2041 gatcgcggcc aacgtcgcgg tattcgtttt cctccagggg ctcggctcga acgaacggtt
     2101 cacctacgcg ttctcgaccg taccgcagga gatcgtcacg gggaaagacg tcgctcaacc
     2161 agtgacgatc aaggaccccg tcagcggcga ggtggcgggc accgtcgagc tcgagccgac
     2221 gccgatcccc gtgtacctga cgctgctgac gtcgatgttc atgcacggag gcatcgcgca
     2281 catcttcggg aacatgctct atctctggat cttcggggac aacctcgagg actcgatggg
     2341 acacctgcga tacctgatct tctacctggt gtgcgggatc atcgcgggcc tgtctcacgt
     2401 cttcgcgacg acggcgctcg gcggcaaccc gtaccttccc gcgctcggag cgtccggcgc
     2461 gatctcggcc gtgctcgccg gctatctcgt gctccatccg aagcggcgcg tgcgggtcat
     2521 catgctgcgg acgatccagg aggttccggc gatctgggcg atcgggatct ggttcgtctt
     2581 ccagctcgtg agcggactcg gaatgctcgg cggtggctcg caggcgggcg gcgtcgcgta
     2641 cgccgctcac atcggcggct tcatcgccgg gctggtcctc gtgaaggtct ttgccgcggg
     2701 gaggccgcgc taccagtaca cgtgacccgg cgcaacgtct tctccggcgc gccctgggag
     2761 ccggtcgtcg gctactcccg cgccgtgcgc gtcggcaaca tggtctgggt gtccggcacg
     2821 acggccaccg acccctcggg aaggatcgcc gcagccggag acgcttacgc gcaggccgtc
     2881 caggcgctcg ccaacattcg gaccgcgctc gagaaggcgg gcgcgtctct cgcggatgtc
     2941 gtgcggacgc gcatgtacgt cgtgaacgtc gccgactggc aagaggtcgg ccgcgcgcac
     3001 gcggagttct tcggggacgt gcgtcccgcc tcgaccctcg tcgaggtgag cgctctcgtg
     3061 tcgccggaga tgctggtcga gatcgaagcc gaggcggtgg tcggcggcgg ggtcagtacc
     3121 tgaggaactg agcccggcga cggcggaact cctccaggcc ctcctcccac gatcttgcga
     3181 ttgcgtcggc ggtctcgccc ctctcgaccc tttcgaacgt gtcccgattg gccaggagag
     3241 aaatgagctt cgcccgctgc cagtccacgg gatagaggtc gcgcagcgcc acggcgatct
     3301 cgatgccgag cgcaaccgga cgcagcgcat cgcgatccgt gaccgtgagc cggactcctc
     3361 cgcagcgctc cccgccgaac gtcgacgacg cgggcgtgaa atggatcggg gtgaacgtca
     3421 cgcccgggat cttccttgcc gcggcgactg ccgcgaggcg ccggccgtcc agccacggtg
     3481 cgccgatcac ctcgaatggc gtgtcggtgc cccttccgat ggagagattc gtcgtctcga
     3541 gcaacccgat cccgggatag agcgtcgcct ccgtcagcga ccgcatgttc ggcgacgggt
     3601 tcacccactc gagcccggtc tcgtcgaacc agagatcccg cgaccagccg gagagcttca
     3661 ccacctcgac gcgcgcgccc gctttctgct cgtcgttgac catcgtgagc agctcccccg
     3721 gcgtcatccc gtatcgcacc ggaatcgtgt ggtgcgccgt gaaggagagc cgatcgggat
     3781 cgctgaccgg cccttcgatg gcctctccgg agatcggatc cggacggtcg agcacgacga
     3841 gcgggatctt cgcgcgcgcg acctcgggaa gcagataccc gcccgtcgtg atgtaggtgt
     3901 agaaacgcgt ccccacgtcc tgcacgtcga ggaggagagc gtcgaggcct tcgagctctc
     3961 cgggcgtcgg tcgccgccgc tctccgtaga gcgaatggat cgtcaagccc gtcgtcggat
     4021 ccacctggtc ggccaccggc gcatcggcgt cggatcggat tccgtgctcc ggcgagaaga
     4081 gagcgacgag cttcacgccg gctttcttcg ctttctccga gacgaggacc tccaccgtcg
     4141 agcgcccgtc gcgggctcgc ccggtcgcgt tggtgacgag gccgacgcgc ttgcccgcga
     4201 ttgcgcggaa accctccgcc tcgagcacgt cgacgccggc caggacctcc cggtgaatcg
     4261 gcgcgcgctc cgcgagctgt cgcgccggct tgcgcgtgtc ctccgtcagc gcggaggcga
     4321 cgattgtcgc gacgagcccc cgcagccggt tgacgtcgcc cttgccgtcg gggtgcagcc
     4381 ggctcgagag gaacacgacg aagaccccgc tggagggatc gatccagaga gacgttccgg
     4441 tgaagccggt gtgtccgaa
//
LOCUS       DASWAV010000486         4185 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U2665
            k141_507543, whole genome shotgun sequence.
ACCESSION   DASWAV010000486 DASWAV010000000
VERSION     DASWAV010000486.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960316
            Sequence Read Archive: SRR10260007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 4185)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4185)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:47:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,929
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4185
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_507543"
                     /isolate="SMAG_U2665"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.36444 N 132.31889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            104..1210
                     /gene="recF"
                     /locus_tag="VF958_10130"
     CDS             104..1210
                     /gene="recF"
                     /locus_tag="VF958_10130"
                     /inference="COORDINATES: protein motif:HMM:TIGR00611.1"
                     /note="All proteins in this family for which functions are
                     known are DNA-binding proteins that assist the
                     filamentation of RecA onto DNA for the initiation of
                     recombination or recombinational repair; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication and repair protein RecF"
                     /protein_id="HEX9149507.1"
                     /translation="MRLLELASRGFRNLCADTVSFGPGVNVVLGENGQGKTNLLEAVA
                     LLCGQRSFRRAKPAEMAADGESFRVEGSLRRGFATEDLAVEWSAGTGRRFRRAGKEIS
                     FREASALAPAVFLAPEHRSILTGPPEERRRFLDRLVVGWRPAAGADLVAYERALKERN
                     ALLNSLREGRSSSSNTPGELDAWTEELATAGEAVRRHRKAALDVWREAFFSLLGTAGK
                     EYSEVTLSYSCGEASDLRTTLKKLLPIERVRGRSLAGPHRDDLVWSRGGRPLEGRASA
                     GEIHRLLTLAKVAEWRVVRDAAGEAPLFGVDDFDAGLSARSLASFLAGLPEATTVVLT
                     SASDPARFRGLAASVLQMEGGRCVGEGARAASGV"
     gene            1221..3641
                     /gene="gyrB"
                     /locus_tag="VF958_10135"
     CDS             1221..3641
                     /gene="gyrB"
                     /locus_tag="VF958_10135"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020678238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009330 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) complex [Evidence
                     IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HEX9149508.1"
                     /translation="MSTAYTTDSIKLLKGLEAVRKRPGMYIGDTDDLSGLHHMVFELV
                     DNSIDEAQVGHADRVSVTIHADNSVTVEDNGRGIPVGMHKEHKRETLEIIMTDLHSGG
                     KFDDNSYKVSGGLHGVGVSVVNALSKRLDVEVRREGKVWVQTYERGIPAAPIRTTGTS
                     RKTGTKITFWADPEIFSVTEFHFDSLSQRLRELSFLNPGVHILIHDERTDKKHDFAYE
                     GGIRSFVELLNKTKQPIHDKVIYFQDVKGATGDPTRDAEKERVEVALQWNEGYTETIF
                     TFTNTINNRDGGTHLEGFKTALTRALQRYAEQNSLTKSLKETNLSGDDCREGLTAVVS
                     VKVKDPKFSSQTKDKLVSSEVKTWVQQVVYEKLGSFLEETPRVSRRIVEKIVESARAR
                     EAARKARELVRRKGALDSGSLPGKLADCQEKNPAFSELFIVEGDSAGGSAKQGRDRRS
                     QAILPLRGKILNVEKARLDKMLANAEIRTLITALGTGIAQDFDVTKIRYHKIVLMADA
                     DVDGAHIRTLLLTFFFRQMPAVIEKGFLYIAQPPLFKVAEGKRETYLKDEKEYSKFLL
                     ARIGEDRALSSEMSGATASGAKLVGLLEKMEEYRTHITRLAGRGVPEGVVRALLDRGM
                     LSKNDFADKKRVEDLAKAVRAFDIEKVDVTADEEHSGWRLEIVQRVSGVPRAASIDAE
                     LIGSYEMKRIRELAKSIGGFLDGPYLVTRNGDSERHSTLPSVVDAIYESAKKGLVVSR
                     YKGLGEMNAETLWETTMDPAKRRLLQVNVEDAVEADLIFSILMGDAVEPRRDFIDKNA
                     LNVRNLDI"
     gene            3667..>4185
                     /locus_tag="VF958_10140"
     CDS             3667..>4185
                     /locus_tag="VF958_10140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012697306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HEX9149509.1"
                     /translation="MSSNLLTPEERVPVTIEEEVRRSYLDYAMSVIIGRALPDVRDGL
                     KPVHRRSLFAMWEQGNLHNKPYKKSARIVGDVLGKYHPHNPDAVYDTIVRMAQDFSMR
                     YPLVDGQGNFGSVDGDNPAAMRYTEVRLTRLAAELIGDDIDKETVDWIPNYDGSTKEP
                     TVLPSKFPNLLVN"
BASE COUNT          780 a         1370 c         1382 g          653 t
ORIGIN      
        1 tgtcatcccg aaggagaggt cgaggccgcg tcgcgctacc tctacgtcgt gatgccgatg
       61 cggctgtgac ccggattcgt cgaaacttcg acctggaaaa aacttgcgcc ttctcgagct
      121 agcttcccgc ggcttccgca acctctgcgc cgacacggtc agcttcggac cgggcgtgaa
      181 cgtcgttctc ggcgagaacg gccaggggaa gacgaacctg ctcgaggccg tcgcgctcct
      241 gtgcggccag cgctcgttcc gtcgagcgaa accggcggag atggcggccg acggcgagag
      301 ttttcgcgtc gaggggtccc tgcgccgcgg gttcgcgacc gaagacctcg ccgtcgaatg
      361 gtcggccgga accggccggc gcttccggcg agcgggaaaa gagatctcgt tccgcgaggc
      421 ctccgccctg gccccggccg ttttcctcgc ccccgagcac cgctcgatcc tgaccggccc
      481 gccggaggag cggcggcggt ttctggatcg gctcgtcgtc ggctggcggc ccgcggccgg
      541 cgcggacctc gtggcctacg agagggcgct caaggagcga aatgcgctct tgaattcgct
      601 gagggagggc cgttcctcgt cttcgaacac ccccggggag ctcgacgcct ggaccgagga
      661 gctggccacc gccggcgagg ccgtccggcg ccaccggaag gccgccctgg acgtctggcg
      721 ggaggcgttt ttcagcctcc tcgggaccgc aggaaaggaa tactccgaag tcaccttaag
      781 ttattcatgc ggagaggctt cagacttacg aacgacactg aaaaagctcc ttccgatcga
      841 gcgcgtgagg ggccggtcgc tggccgggcc gcaccgagac gacctggtct ggagccgagg
      901 cgggaggccc ctcgaaggcc gggcatcggc cggcgaaatc caccggctcc tgaccctggc
      961 caaggtcgcg gagtggcggg tcgttcgcga cgctgccggc gaggcgccgc tcttcggggt
     1021 cgacgacttc gatgcgggtc tctcggcgcg ttcgctcgcg agttttctcg cggggctgcc
     1081 ggaggcgacg acggtcgtcc tgacgtccgc gtcggacccg gcgcgtttcc gcgggctcgc
     1141 cgcgtcggtt ctccagatgg aagggggccg atgcgttggc gagggcgcgc gcgccgcctc
     1201 cggagtttga gtcaaggaac atgagcaccg cttacaccac cgactcgatc aagctcctga
     1261 aaggcctcga ggccgtccgc aagcggcccg ggatgtacat cggcgacacc gatgacctct
     1321 cgggcctcca ccacatggtc ttcgagctcg tggacaactc gatcgacgaa gcccaggtcg
     1381 ggcacgcgga ccgcgtatcg gtgacgatcc acgccgacaa ctccgtcacc gtcgaagaca
     1441 acgggcgcgg catcccggtc gggatgcaca aggagcacaa gcgcgagacg ctcgagatca
     1501 tcatgacgga ccttcactcg ggcggaaagt tcgacgacaa ctcttacaag gtctcgggcg
     1561 gcctgcacgg ggtcggcgtc tccgtcgtga acgcgctctc gaagcggctc gacgtcgagg
     1621 tgcgccgcga ggggaaggtc tgggtgcaga cctacgagcg cggcattccg gccgcgccga
     1681 tccgcacgac cggaacgtcg cggaagaccg ggacgaagat cacgttctgg gccgacccgg
     1741 agatcttctc ggtcaccgag ttccacttcg acagcctctc gcagcggctg cgcgagctct
     1801 ccttcctgaa tcccggcgtc cacatcctga tccacgacga gcgcaccgac aagaagcacg
     1861 actttgccta cgagggcggg atccggtcct tcgtggagct cctgaacaag acgaagcagc
     1921 cgatccacga caaggtcatc tatttccagg acgtcaaggg cgccacgggc gacccgacgc
     1981 gcgacgccga gaaggagcgc gtggaggttg cgctccagtg gaacgagggt tacaccgaga
     2041 cgatcttcac gttcaccaac acgatcaaca accgcgacgg cggcacgcac ctcgaaggct
     2101 tcaagaccgc gctgacgcgc gctctccagc gctacgccga gcagaacagc ctcacgaagt
     2161 cgttgaaaga aacgaacctc tcgggagacg actgccgcga gggcctgacc gcggtcgtct
     2221 cggtgaaggt gaaagacccg aagttctcct cccagacgaa ggacaagctc gtctcctcgg
     2281 aggtcaagac gtgggtccag caggtcgtct acgagaagct cgggtccttc ctcgaggaga
     2341 cgccgcgggt ctcgcgccgc atcgtcgaga agatcgtgga gtcggcgcgg gcgcgcgagg
     2401 cggcgcgcaa ggcgcgcgag ctcgtgcgtc ggaagggcgc gctcgacagc gggtcgctcc
     2461 ccggcaaact cgccgactgc caggagaaga accccgcgtt ctccgagctc ttcatcgtcg
     2521 agggtgactc ggccggcgga tcggccaagc aggggcgtga tcgccgttcc caggcgatcc
     2581 tgccgttgcg cggaaagatc ctgaacgtcg aaaaggcgcg gctggacaag atgctcgcga
     2641 acgccgagat ccgcacgctc atcaccgcgc tcggcaccgg gatcgcgcag gacttcgacg
     2701 tcacgaaaat ccggtaccac aagatcgttc tgatggccga cgcggacgtc gacggggcgc
     2761 acatacgcac gcttcttctg acgttcttct tccgccagat gccggcggtc atcgagaagg
     2821 ggttcctcta catcgcgcag cccccgctct tcaaagtcgc ggaggggaag cgcgagacgt
     2881 acctgaagga cgagaaggag tattcgaagt tcctcctcgc ccggatcggg gaggaccgcg
     2941 cgttgagctc cgagatgtcc ggggcgacgg cgtcgggagc gaaactcgtc gggcttctcg
     3001 agaagatgga ggagtaccgc actcacatca cgcgcctcgc cggccgcggc gttccggaag
     3061 gcgtcgtgcg cgctctgctc gaccgcggga tgctctcgaa gaacgacttc gcggacaaga
     3121 agcgcgtcga ggacctcgcg aaagcggtgc gcgcattcga catcgagaag gtcgacgtca
     3181 ccgccgacga agagcactcc ggctggcgcc tcgagatcgt tcagcgagtc tcgggcgtcc
     3241 cgcgcgccgc atcgatcgat gccgagctca tcggctccta cgagatgaag cggatccgag
     3301 agctcgcgaa gtcgatcggc ggcttcttgg acggccccta cctcgtgacc cgcaacggag
     3361 acagcgaacg ccactcgact ctcccctcgg tcgtggacgc gatctacgag tccgcgaaga
     3421 aaggcctcgt cgtctctcgc tacaagggtc tcggcgagat gaacgcggag acgctgtggg
     3481 agacgacgat ggatcccgcg aagcggcgcc tgctccaggt caacgtcgag gacgcggtcg
     3541 aggccgacct gatcttctcg atcctcatgg gagacgctgt cgagccgcgc cgcgatttca
     3601 tcgacaagaa cgcgttgaac gtgcgcaacc tcgacatttg acgaatcgtc ccctcagggc
     3661 tgaagaatga gcagcaacct actcaccccc gaagagcgcg tccccgtcac gatcgaggag
     3721 gaggtccgcc gctcctacct cgactacgcg atgtcggtca tcatcgggcg ggcgctgccg
     3781 gacgttcggg acggtttgaa gccggtccac cgccggtccc tcttcgcgat gtgggagcag
     3841 ggaaacctcc acaacaagcc ttacaagaag tccgcccgca tcgtcggaga cgtcctcgga
     3901 aagtaccacc cgcacaatcc cgatgcggtc tacgacacga tcgtgcgcat ggcgcaggac
     3961 ttttccatgc gttatccgct ggtggacggc cagggaaact tcgggtcggt ggacggcgac
     4021 aacccggccg cgatgcgcta caccgaggta cggctgacgc ggctcgccgc ggagttgatc
     4081 ggggacgaca tcgacaagga gacggtggat tggattccca actacgacgg ctcgacgaaa
     4141 gagccgaccg tgctcccctc taagttcccg aacctgctcg tcaac
//
LOCUS       DASWAV010000487         3195 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U2665
            k141_666410, whole genome shotgun sequence.
ACCESSION   DASWAV010000487 DASWAV010000000
VERSION     DASWAV010000487.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960316
            Sequence Read Archive: SRR10260007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 3195)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3195)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:47:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,929
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3195
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_666410"
                     /isolate="SMAG_U2665"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.36444 N 132.31889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..871
                     /locus_tag="VF958_10145"
     CDS             <1..871
                     /locus_tag="VF958_10145"
                     /inference="COORDINATES: protein motif:HMM:NF021126.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="YfhO family protein"
                     /protein_id="HEX9149510.1"
                     /translation="LLLVGQRFFEMRGTYPTLPERALAPPLSIFSALPKTADPYRIVA
                     AGDTFRPNAAALYGLEDVRGYESLVLDRFAETYPMWCRPQFASFNRVDDLTRPFLSFV
                     NVRYGIADPATPALPGWALVARTREGSVFENPRALPRAFVPAKVRYQPDEKKQLAEMA
                     ETKDFGEVAWLGRPPHPNPLPEGEGANGRASVTVREVGTDLIVEADVRERTLVATSIP
                     DWPGWRAKAGSADVPLATVNHAFVGFWLAPGRQVVRLHYLPSSFLLGTLLSAGAVIAV
                     SLAAVLRRRRGLR"
     gene            878..>3195
                     /locus_tag="VF958_10150"
     CDS             878..>3195
                     /locus_tag="VF958_10150"
                     /inference="COORDINATES: protein motif:HMM:NF021126.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhO family protein"
                     /protein_id="HEX9149511.1"
                     /translation="MFLLVVRLLAVYFATCALAFFLVNRFVAKVRPGIAILLSLGPFL
                     LTGKAMVTAGIYAPLDIAYQAHPLAAHREEMGIGRTRSPILGDVVYQEIPWRKAAREA
                     MKNGRLPLWNRFPLAGEPLLAVQQPAVLHPSTWIGFLLPLAQAWTFEMSFRHFLALLA
                     MYLFLRELGCGELGALLGAAGWGFSDYLVFFAGYPLSPAAAPFPLLLLGLRRLAREPN
                     RVAVGITVVALLLNVTSGHPETLLHAVAGAGVYFLFELAFTERRRRSKALGLSLVAGA
                     ITLGLSAVLLLPLAEALPQTAEHFFRSGFYAHLDKSVSVAESLRRSIPDVVPYAFGVS
                     GHGRSAIGFGEPAAYVGAVLLPFAILGLFSRRRETWPLLVITFVGLAMWARLPVVTDA
                     VSSLPLFDIGINERLVFLAAFGISALAGLGAERFRQKDGFSLVFATLATGVVVFLLHR
                     EVGPTLARLEMDPGFLRGRLLAEVVPLVLLAVVAAISLPWKRMAPVLFSALAILLVQR
                     RIEAGDLYPTFPNRTFYPDLAVLDPIPRHEPWRFVATGYQFIPNIAALYELEDVRGYE
                     AMTFLPLTGTFPLWCVPQPIWFNRVDDPTTPFLAFLNVRWVLESADWKPPSGWKALAE
                     GDGTRLFENPRALPRVFVPRMVRATPVEQQREALASIADFSERGIVVGTGPDWPPGEW
                     RPNGAATVRIESYRPQRLELAVDARAKAVVGTSIPRWPGWRLEIDGRRAPLFTYNTAF
                     LGFEVPARSRLAELRYLPDGFLAGYAISAGTIA"
BASE COUNT          389 a         1150 c         1040 g          616 t
ORIGIN      
        1 cctccttctc gtcggtcagc gcttcttcga gatgcgtggc acctacccga ccttgccgga
       61 gcgggcgctc gcgccaccgc tctcgatttt ctccgcgctc ccgaagaccg cggacccgta
      121 ccggatcgtc gccgcgggcg atacgttccg cccgaacgcc gcggcgctct acggcctcga
      181 ggacgtgcgc ggttacgaat cgctggtgct cgaccggttc gccgaaacgt atccgatgtg
      241 gtgccggcct caattcgcgt cgttcaaccg cgtggacgat ctcacccgtc cgttcctgtc
      301 gttcgtcaac gtccgctatg ggatcgcgga cccggcgacc ccggcgcttc ccggatgggc
      361 gctcgttgcc cggacacgcg aaggttccgt tttcgagaac ccccgcgcgc tgccgcgggc
      421 gttcgtgcct gcgaaggtgc gatatcagcc cgacgagaag aagcaactgg cggagatggc
      481 ggagacgaag gacttcgggg aggttgcgtg gctcggtcgg ccccctcacc ccaaccctct
      541 cccggaggga gagggagcaa acggccgcgc aagcgtgacg gttcgcgagg tcgggacgga
      601 cctgatcgtc gaagccgacg tccgcgagcg cacgctcgtg gccacgtcga ttccggactg
      661 gccgggctgg cgggcaaagg ccgggagtgc cgacgttcct ctcgcgaccg tgaaccacgc
      721 cttcgtcggc ttctggctgg ctccgggccg gcaggtcgta cggttgcact acctgccttc
      781 gtcgtttctt ctcgggacgc tcctctcggc gggggccgtg atcgccgtct cgctcgccgc
      841 agtccttcgc cgccgacgcg ggttacgcta gccgcgcttg tttcttctcg tcgtccgcct
      901 cctcgccgtc tacttcgcga cctgcgcgct cgctttcttc ctggtgaacc gtttcgtcgc
      961 gaaggtccgg ccggggatcg cgattcttct ttcgctcgga ccctttcttc ttaccgggaa
     1021 ggcgatggtg accgccggca tctacgcgcc gctcgacatc gcgtaccagg cgcacccgct
     1081 cgcggcgcac cgggaggaga tgggaatcgg caggacgcgc tctccgatcc tgggcgatgt
     1141 cgtctaccag gagattccgt ggaggaaagc cgcccgcgaa gccatgaaga acggacgcct
     1201 ccccctctgg aaccgcttcc cgctggcggg ggagccactg ctcgcggtcc agcagcccgc
     1261 ggttctccac ccgtcgacgt ggatcggatt cctgctgccc ctcgcgcagg cctggacgtt
     1321 cgagatgtct ttccggcact tcctcgccct cctggcgatg tacctttttc tgcgcgagct
     1381 cggctgcggt gagcttgggg ctctcctcgg cgcggcggga tggggtttct ccgactacct
     1441 cgtgttcttc gcgggatatc cgctttcgcc agccgcggcg cccttcccgc tcctcctgct
     1501 gggcctccgg cgcctcgcac gcgagccgaa ccgcgtcgct gtgggcatca ccgtcgtcgc
     1561 cctgctcctg aacgtcacct cgggtcaccc cgagacgctc ctgcacgcgg tcgcaggtgc
     1621 gggcgtctac tttctcttcg agctcgcgtt caccgaacga cggcgtcgtt cgaaggcgct
     1681 cgggctgtcg ctcgtcgcag gcgcgatcac gctcggcctg tccgccgttc tccttctgcc
     1741 gctcgcggag gcgttaccgc aaaccgccga gcacttcttc cgatccggct tctacgcgca
     1801 cctcgacaaa tccgtcagcg tcgccgagag cctgcggcgc tccattccgg acgtcgttcc
     1861 gtacgccttc ggcgtctcgg ggcacggtcg atcggcgatc ggcttcggcg agccggcggc
     1921 gtacgtcggc gccgtgctgc tcccgttcgc catcctcggt ctcttctcgc ggcgtcggga
     1981 aacgtggccg ctcctcgtca tcacgttcgt gggcctcgcc atgtgggcgc gtcttcccgt
     2041 cgtcaccgac gcggtctcct cgcttccgct cttcgacatc ggcatcaacg agcggctcgt
     2101 cttcctcgcg gcattcggga tctcggcgct cgcgggcctc ggggcggagc ggttccgcca
     2161 gaaggacggc ttctcgctgg tcttcgcgac tctggcgacc ggggtcgtgg tctttctgct
     2221 gcaccgggag gtcgggccga cgctcgcccg actcgagatg gatcccgggt tcctgcgggg
     2281 ccggctgctg gccgaggtcg tcccgctggt tctgctcgcg gtcgtcgcgg cgatctcgct
     2341 gccttggaaa cgtatggcgc cggtgctctt ttcagccctc gcgatcctcc tcgtgcagcg
     2401 gagaatcgaa gcgggcgatc tctacccgac gttcccgaac cggaccttct accccgacct
     2461 cgccgtgctc gatccgattc cgcgccacga gccgtggcgt ttcgtggcga cgggctacca
     2521 gttcatcccg aacatcgccg cgctctacga gctcgaggac gtgcgcggct acgaggcgat
     2581 gacgttcctg ccgctcaccg ggacctttcc gttgtggtgc gttccgcagc ccatctggtt
     2641 caaccgggtg gacgacccga cgactccgtt tctcgcgttc ctgaacgtcc ggtgggtgct
     2701 cgagtccgcc gactggaagc cgccgtccgg ctggaaggca ctcgcggaag gggacggaac
     2761 ccggctcttc gagaacccgc gcgcgctgcc acgtgtgttc gtcccgcgga tggtgcgggc
     2821 gacgccggtc gagcagcagc gcgaagcgct ggcctccatc gcggacttct ccgagcgggg
     2881 catcgtcgtc ggcacgggcc cggactggcc cccgggagag tggcggccca acggagccgc
     2941 gacggtgcga atcgagtcct accggccgca gcgactcgag ctcgccgtgg acgcccgggc
     3001 gaaggcggtc gtcgggacct cgatcccgcg gtggcccggg tggcggctcg agatcgacgg
     3061 gcggcgtgcg ccgctcttca cctacaacac cgcgtttctc ggcttcgagg tccccgcgcg
     3121 atcccggctt gccgagctgc gctacctccc cgacggattt ctcgctggct acgcaatctc
     3181 cgcggggacg atcgc
//
LOCUS       DASWAV010000488         3029 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U2665
            k141_708112, whole genome shotgun sequence.
ACCESSION   DASWAV010000488 DASWAV010000000
VERSION     DASWAV010000488.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960316
            Sequence Read Archive: SRR10260007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 3029)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3029)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:47:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,929
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3029
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_708112"
                     /isolate="SMAG_U2665"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.36444 N 132.31889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..152
                     /locus_tag="VF958_10155"
     CDS             <1..152
                     /locus_tag="VF958_10155"
                     /inference="COORDINATES: protein motif:HMM:NF012780.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEX9149512.1"
                     /translation="LAEAAQTLFENKIGCLPVVEGSRLVGILTESDFVRRFVEEEAGR
                     EGKDS"
     gene            185..772
                     /locus_tag="VF958_10160"
     CDS             185..772
                     /locus_tag="VF958_10160"
                     /inference="COORDINATES: protein motif:HMM:NF016184.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein tyrosine phosphatase family protein"
                     /protein_id="HEX9149513.1"
                     /translation="MKRLVLLPGLAAAAMLLAVFGFGATCADINKFYWIDERIATGGQ
                     PKVSDLASLKKEGFKTIINLREASEYDAAAEEAEARRLGLGYVSIPVRTASPKDEQAD
                     AFLAALSDRKIFPAFIHCGTANRVGAFWMIARVLVDGWNITDAEHEARVIGMKSSNLR
                     EFAYEYICRHQKADSPAVPCANRPSGTEDHKARTD"
     gene            840..1265
                     /locus_tag="VF958_10165"
     CDS             840..1265
                     /locus_tag="VF958_10165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010959563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="archease"
                     /protein_id="HEX9149514.1"
                     /translation="MAWEHFEHGADVGIRGRGDTAARAFGEAARALFSLLSDDLTAIR
                     LSVEERVACEADNLEELLVAFLNELLFLFDARRVVFARFDVGIEEGPGCFRLTAIAHG
                     EPFDPSRHEFTVLPKGATFTALEVGKHDGEWVAQCVVDV"
     gene            1272..2723
                     /locus_tag="VF958_10170"
     CDS             1272..2723
                     /locus_tag="VF958_10170"
                     /EC_number="6.5.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011431330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008452 - RNA ligase activity [Evidence
                     IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RtcB family protein"
                     /protein_id="HEX9149515.1"
                     /translation="MSIERAEGSALGFHEVEPNVWQLEPTGEMRVPGRIFASRDLIDA
                     LDEKVREQLANVAALPGIVGASLAMPDAHWGYGFPIGGVAAFDPERGGIVSAGGVGFD
                     ISCGVRTFTTGMKAAELGDRLETVAHDLYRTVPAGVGWGGAVKLTVPELDRALAGGAV
                     WAVEKGWGEAEDLGRIEEGGRMEGAEPSRVSARAKERQLHEVGSLGSGNHYLELQVVE
                     RIFDSEAAAAFRLAENDLLVTIHCGSRGLGHQIGTEYLQKFVPVARRYGISLPDRELA
                     CAPIRSPEGEEYLGAMRAAINCALANRQVIGALVRKVFARHFPSTKLELLYDVSHNTC
                     KAEEHEVDGKTRLLFVHRKGATRAFGPGHPDVPPEYRAVGQPVLIGGTMGTASFVLAG
                     TSLSESRAFSSSCHGAGRKMSRTRAKTLWQGKPLIRELAGRGIVIKSHSAKGAAEEAP
                     GAYKDVDEVARVAEEVGLSRRVARVVPIACVKG"
     gene            complement(2802..>3029)
                     /locus_tag="VF958_10175"
     CDS             complement(2802..>3029)
                     /locus_tag="VF958_10175"
                     /inference="COORDINATES: protein motif:HMM:NF012892.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporting ATPase C-terminal
                     domain-containing protein"
                     /protein_id="HEX9149516.1"
                     /translation="TAGNPLRSRPSAPLAITTLAVVVVAVLLPYTPAARALGFEPLPA
                     AFFAFLLAVVGAYLFFVELVKRHLLRRPAPG"
BASE COUNT          521 a          980 c         1069 g          459 t
ORIGIN      
        1 cgctcgccga agcggcacag acgctgttcg agaacaagat cgggtgtctt cccgtcgtcg
       61 agggaagtcg cctcgtcggg atcctgaccg agtcggactt cgtgcgccgc ttcgtcgagg
      121 aagaagccgg gcgcgaagga aaggactcct gaaaatcggt ccggatcgga actgtgaggt
      181 ccatatgaaa cgcctggttc ttctccccgg tctcgctgct gccgcgatgc tcctcgccgt
      241 cttcggcttc ggagcgacgt gcgccgacat caacaagttc tactggatcg acgagcggat
      301 cgcgacgggc ggccaaccga aggtctccga cctcgcttcg ctcaagaaag agggcttcaa
      361 gacgatcatc aacctgcggg aggcctcgga atacgacgcc gccgccgaag aggcggaggc
      421 gcgccggctg gggctcggct acgtctcgat cccggtccgg accgcctcac cgaaggacga
      481 gcaggccgac gccttcctcg ccgctctgtc cgacaggaag atctttccgg ccttcatcca
      541 ctgcggcacg gccaaccgcg tcggagcgtt ctggatgatc gcaagggtcc tcgtcgacgg
      601 ctggaacatc accgacgccg agcacgaagc gcgcgtgatc ggaatgaaga gctcgaacct
      661 ccgcgaattc gcctacgagt acatctgccg tcatcagaag gcggattcac ccgcggttcc
      721 ctgtgcgaat cgcccgtcgg gaacggagga tcacaaggcg aggacggact agactctcgt
      781 ccgcgactcg gatgcccggc gcggctcccg aagggaaccg ggcccggcgt ccgccagcga
      841 tggcctggga gcatttcgag cacggcgcag acgtcgggat acgcggccgc ggagacacag
      901 cggcgcgcgc cttcggggag gccgctcggg cgctcttctc cctcctctcc gacgatctca
      961 ccgcgatccg gctctcggtc gaggagaggg tcgcgtgcga agcggataat ctcgaggagc
     1021 tgctcgtcgc gttcctgaac gagctgctgt ttctcttcga tgcccggcgg gtcgtgttcg
     1081 cgcgattcga cgtcgggatc gaggagggcc ccgggtgctt tcgcctgacg gcgatcgcac
     1141 acggcgagcc tttcgatccg agccgtcacg aattcacggt gctccccaag ggcgcgacgt
     1201 tcacggcgct cgaggtcgga aaacacgacg gagaatgggt ggcgcaatgc gtcgtagacg
     1261 tctaggcagc agtgtcgatc gagcgcgctg aaggttcggc tctcggattc cacgaggtgg
     1321 agccgaacgt ctggcagctc gagccgacgg gtgagatgcg cgtccccggc cggatcttcg
     1381 cgagccggga cctgatcgac gcgctcgacg agaaggtgag agagcagctc gccaacgtcg
     1441 cggcgcttcc cgggatcgtc ggcgcctcgc tcgcgatgcc ggacgcccat tggggctacg
     1501 gctttccgat cggcggcgtc gccgcgttcg atccggagag gggcgggatc gtgtcggcgg
     1561 gcggcgtcgg cttcgacatc tcgtgcggcg tgcgcacgtt cacgaccggc atgaaggccg
     1621 ccgagctcgg agaccgcctc gagacggtcg cgcacgacct ctaccgcacc gtcccggcgg
     1681 gggtgggctg gggcggcgcc gtgaagctga ccgttcccga gctcgaccga gcccttgcgg
     1741 gaggagcggt gtgggccgtc gagaagggct ggggcgaggc cgaggacctc gggcggatcg
     1801 aggagggtgg gcggatggaa ggtgcggagc cttcgcgcgt cagcgcgcgc gcgaaggagc
     1861 ggcaactcca cgaagtcgga agcctcggct cgggaaacca ctacctcgag ctccaggtgg
     1921 tcgagcgcat tttcgactcc gaagccgcgg ccgcgttccg gctcgcggag aacgacctcc
     1981 tggtgacgat ccattgcggc tcacgagggc tcggacacca gatcgggacc gagtacctcc
     2041 agaagttcgt cccggtcgcg agacgatacg gaatttccct gccggaccgg gagctcgcgt
     2101 gcgcgccgat ccgctctccc gaaggcgagg agtacctggg cgcgatgcgg gccgcgatca
     2161 actgcgccct cgccaaccgc caggtcatcg gcgcgctcgt acgaaaggtc ttcgcgcgcc
     2221 actttccgtc gacgaagctc gagctcctct acgacgtctc gcacaacacg tgcaaagcgg
     2281 aagagcacga ggtggacggg aagacgcggc ttctcttcgt ccatcgcaag ggagcgaccc
     2341 gagcgttcgg gccgggccat cccgacgttc cccccgaata ccgcgcggtc ggccagccgg
     2401 ttctgatcgg cgggaccatg gggacggcgt cgttcgttct cgcggggacg tctctgtcgg
     2461 agtcgcgcgc gttcagctcg tcctgccacg gcgccggccg gaagatgagc cgcacgaggg
     2521 cgaagactct gtggcaggga aagcccctca tccgggagct cgccgggagg ggcatcgtca
     2581 tcaagtccca ctcggcgaag ggagcggccg aggaagctcc cggcgcctac aaggacgtcg
     2641 acgaggtcgc cagggtcgcg gaggaggttg gtctctcgcg gcgggtcgcc cgggttgtgc
     2701 ccatcgcgtg cgtcaagggg tgacgcggcg atcaatgtcc gggacccgcg ccgctctgcc
     2761 ttgagaacca ccgccgacga agttcagatc ccggccttgc gtcagccagg cgccgggcga
     2821 cgcagaagat ggcgcttcac gagctccacg aagaagagat acgcgccgac gaccgcgagg
     2881 agaaacgcaa agaaagccgc gggcagcggc tcgaacccga gagcccgcgc tgcgggcgtg
     2941 tacggcagaa ggacggcgac cacgacgacc gcaagcgtcg tgatcgcgag cggcgcgctc
     3001 gggcggctcc ggagcggatt gcctgccgt
//
LOCUS       DASWAV010000489         1897 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U2665
            k141_467608, whole genome shotgun sequence.
ACCESSION   DASWAV010000489 DASWAV010000000
VERSION     DASWAV010000489.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960316
            Sequence Read Archive: SRR10260007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 1897)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1897)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:47:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,929
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1897
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_467608"
                     /isolate="SMAG_U2665"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.36444 N 132.31889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(1..296)
                     /locus_tag="VF958_10180"
                     /pseudo
     CDS             complement(1..296)
                     /locus_tag="VF958_10180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006982728.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding domain-containing protein"
     gene            388..462
                     /locus_tag="VF958_10185"
     tRNA            388..462
                     /locus_tag="VF958_10185"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:420..422,aa:Asn,seq:gtt)
     gene            complement(517..768)
                     /locus_tag="VF958_10190"
     CDS             complement(517..768)
                     /locus_tag="VF958_10190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9149517.1"
                     /translation="MSYRRNIFRPRSAADVSKAFTCSFCRSVSHWTRVTPDQAMYCLS
                     CRCGYCAGLLAFQSVDEGKPNLYCHRCIREGDAPAAVVN"
     gene            complement(765..896)
                     /locus_tag="VF958_10195"
     CDS             complement(765..896)
                     /locus_tag="VF958_10195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9149518.1"
                     /translation="MSMENWPVEWALVLEALLPCLLLLAVVMIVAYTLMAKFSEDRQ"
     gene            complement(924..1130)
                     /locus_tag="VF958_10200"
     CDS             complement(924..1130)
                     /locus_tag="VF958_10200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9149519.1"
                     /translation="MNDTVFAFRADGVVKIIRKLEAATRLAKALEEVLPAMYGIGDKE
                     RVEQEALVALATWEEASREEGSWN"
     gene            1412..>1897
                     /locus_tag="VF958_10205"
     CDS             1412..>1897
                     /locus_tag="VF958_10205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006101724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyltransferase family protein"
                     /protein_id="HEX9149520.1"
                     /translation="MLFRDRAEAGRRLAERLRHYAGRPDVVVLALPRGGVPVASEVAR
                     ELGAPLDVFLVRKLGFPGQEELAMGAIASGGSRVLNRSLLRRLHVPEETVNAVAAREF
                     CEIERRERAYRGTRPPRPVRGRTVILVDDGLATGATMHAAAAALRGQDPARIVVAVPV
                     AA"
BASE COUNT          297 a          630 c          626 g          344 t
ORIGIN      
        1 gtcgcgttca cagtgttcaa cgccttgacg acgcgcgcct ccggatacgc cttctggatg
       61 cgctcgccga gcgagtcgtt tgcgccggtg aagagagacg gcggcatccc gcgcgaaaaa
      121 tcgagcgggt tggccacgtc cacgaggacc ttgccgcgca ggttcgctgc tcccgccgct
      181 ttgagggcgt cgagcgaccc cgcgccgctc gtgcagttga agacgatctc gccgaacgcc
      241 gccgcgtcgg cgaacgtgcc gtgcgacgcc tttgcgccaa ccgattttgc ccagtccgcc
      301 gacgagggtc gcgggcaagg tcccggtgtt tgacaggccg ggacggcgtc cgctatcttt
      361 ctcgcccgct tcgagcgagc gctctgttcc tcagtagctc aatggcagag catccggctg
      421 ttaaccggag ggttgtaggt tcaagtccta cctgaggagc caattcctcc gccgcgtcga
      481 acgatcttcg agctcttcgc gtccagcggt tcttcgtcag ttgacgactg ccgccggcgc
      541 gtcgccttcc cggatgcacc tgtggcagta gaggtttggt tttccctcgt ccaccgactg
      601 gaacgcgagc aggccagcgc agtatccgca ccgacaggag aggcaataca tcgcctgatc
      661 tggcgtcacc cgcgtccagt gggacacgct cctgcagaac gagcaggtga acgccttcga
      721 gacgtcggca gccgaacgcg ggcggaagat gttgcgccga tagctcactg ccgatcctcg
      781 gagaacttag ccatcagggt gtaggcgacg atcataacca cagcgagcag gagcaggcag
      841 ggcaacaggg cttccagcac cagcgcccat tcgaccggcc agttctccat cgacatcaga
      901 aggcgtcgct gtccgaacgg tccctagttc cagcttcctt cctcgcgcga agcctcctcc
      961 caggtcgcga gggccacgag cgcttcctgc tcgactcgct ccttgtcacc gatgccgtac
     1021 atggcgggaa ggacttcctc gagagccttc gcgagtcgag tcgccgcctc gagcttgcgg
     1081 atgatcttga cgactccatc ggcccgaaag gcgaacactg tgtcgttcat cttcgtcctc
     1141 ttgaacagcg cggccacctg gccgggcacg ttatcggggc cgacttcccc ggcccggttc
     1201 gtcctttgca acgggcgtac cctggctccg atcgctcctc ggccgggcgc ggcttttgca
     1261 ctttcgtctt ctggcggtaa acgtctggag agaaaaggac gaccggattc gcgccaacgc
     1321 gccggatcgt cgcgacgcct cgtcgtcgcc gggccttcgc cggtcgcatt cgtccgccgg
     1381 cgtttccgtc gggcgaaagg agtgatcgca catgttgttc cgtgaccgcg ccgaggccgg
     1441 gagacgtctc gcggagaggc tgcggcacta cgcgggaaga cccgatgtcg tcgtcctggc
     1501 cctgccgcgc ggaggagttc ccgtcgcgag tgaagttgcg cgcgagctcg gcgcgcccct
     1561 cgacgtcttt ctcgtccgaa agctcggctt tcccgggcag gaggagctcg cgatgggggc
     1621 gatcgccagc ggcggatcgc gcgtgttgaa ccggagcctc ctgcgccgcc tgcacgttcc
     1681 ggaggagacc gtgaacgcgg tggccgcgcg cgagttctgc gagatcgagc gtcgggagcg
     1741 tgcttatcgg ggaacgagac cgccgcggcc ggtccgcgga cggaccgtga tcctggtgga
     1801 cgacggtctg gcgacgggcg cgacgatgca cgcggccgca gctgctctgc gcgggcagga
     1861 tccggcgcgc atcgtcgtcg cagttccggt agcggca
//
LOCUS       DASWAV010000490         4819 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U2665
            k141_467676, whole genome shotgun sequence.
ACCESSION   DASWAV010000490 DASWAV010000000
VERSION     DASWAV010000490.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960316
            Sequence Read Archive: SRR10260007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 4819)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4819)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:47:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,929
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4819
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_467676"
                     /isolate="SMAG_U2665"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.36444 N 132.31889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1375
                     /locus_tag="VF958_10210"
     CDS             <1..1375
                     /locus_tag="VF958_10210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9149521.1"
                     /translation="PALGGSPRRIAESGNFPSWSSDGSSLVFMGGGAFKKKILRVHAS
                     GGEAQEFAIRPPKGVAGFRFILYPTLSPDGKWILFQAEQFRVHVVPSSGGEAKLLCRG
                     RRPVWAPDGRSVVYSNADPGKNYSLWRIPFSPAKGAADGPAEPVTVGRGVDTQATISH
                     DGRQIAFTALEQTFNLETLPFDAELGRATGEVKVLTSGNNDIEWKSLSPDGRSVVYGN
                     AGRVWRLDPGQAAYPLTSDPAFIDGQPRWSPDGRSIIFERAPAGEPGNFSIWLMAADG
                     AGPRKLLEKARSGYWMPDSRGITYSSLSDFYLYHFDLATRRSRRVADEETRAPLHLVS
                     NDGKWIVYQSSAQSSITIHAHPIDGGETRVVASTPGQMGSGHPMLSPSGNWFYYQVDH
                     KNVYRLPGPSQGWKKAEPQKVTNFPESGLFIEDPRLSMDGKQLIYSRGKITGDLWLLR
                     FGKPGSK"
     gene            complement(1379..2308)
                     /locus_tag="VF958_10215"
     CDS             complement(1379..2308)
                     /locus_tag="VF958_10215"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HEX9149522.1"
                     /translation="MRPRISSPLRGRTLVVYILLCAIWGSTWLSIKVGLEYLPPLRFA
                     ALRMALACLVLAPFAFRHRDRRPSAREAAFIALAGFLQIGVFYGLIFVAQQWIESGLA
                     ALLFATFPICAGVFAHFLLPDEPLTPRTIAAAGLGLSGVALIEGAALWRALQADFGTL
                     LRGGGLVFVAAIVAAFASVIVKKHLGRVDPIVNVWGETLVGAAFLLPLAALVERGATA
                     RWTPPAIGSLAYLAIFGTAITFGGLFWLIKRVPISVVSTIPLVDTLIAVLLGAAFLGE
                     RLPARALAGGALILLGVFLATRGGSRTEAAARA"
     gene            2391..2888
                     /locus_tag="VF958_10220"
     CDS             2391..2888
                     /locus_tag="VF958_10220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010186545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0033745 - L-methionine-(R)-S-oxide
                     reductase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HEX9149523.1"
                     /translation="MFEVRSDPNLSEGERYRDLEAALVSLIDGETDALANLANAAALL
                     AHSLEAINWCGFYLLRGEELVLGPFQGKPACIRIPLGRGVCGRAALERATLVVPDVSR
                     FPDHITCDPASRSEIVVPIVEGDVLRGVLDVDAPEVGRFDREDLAGLERFVAALAPRI
                     EWERL"
     gene            2889..3611
                     /locus_tag="VF958_10225"
     CDS             2889..3611
                     /locus_tag="VF958_10225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013907798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HEX9149524.1"
                     /translation="MGQKQFGGKVTWLGHACFLIESPKGVRIAIDPWIGNNPRFPKAF
                     EWGRLDVIAATHGHFDHFGDDGLALAKKTGATVIAIFELALHCGANGVEKVSGMNKGG
                     SQKVSGVEFRMVNAIHSSGTSGAGKETNFPADPCGYVITFEDGFRIYHAGDTNVFADM
                     ALYGELYEPDLALLPIGDFYTMGPREAAKACQLLGIPRVIPIHWGTFPALPGTPAAFR
                     REVDRRGLPTQVIELSPGQSWP"
     gene            3624..4016
                     /locus_tag="VF958_10230"
     CDS             3624..4016
                     /locus_tag="VF958_10230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9149525.1"
                     /translation="MRKPIRFLPTLLALGLAVACVSTRADWQLLGRKDVSFLVERDTI
                     DVERSEGRLHALKIVVEGAPVEIRDIRVTFGDGSTFHPETRLTFAAGSESRSIDLPGR
                     GRVIRKIDFVYRKTSGIFRQATVSVYGR"
     gene            complement(4053..4535)
                     /locus_tag="VF958_10235"
     CDS             complement(4053..4535)
                     /locus_tag="VF958_10235"
                     /inference="COORDINATES: protein motif:HMM:NF025852.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4437 domain-containing protein"
                     /protein_id="HEX9149526.1"
                     /translation="MRMNARNVLSFVSFFVLALAVLAQGTKSVEGAEVISPTPMPAAD
                     LHWTDLDPAGAPGAKVADLWGSHQKGAFGAFFRLPAGFATPLHTHTHDMKVVIVSGTY
                     IQRPEGKPEFRLGPGSYLMQPGGSYRHTTSCDQASECLFFVESNGAFDLQPAVAAAPK
                     "
BASE COUNT          851 a         1593 c         1610 g          765 t
ORIGIN      
        1 gccggcgctg ggcggaagtc cgcgccggat cgccgagtcg ggcaactttc cgtcctggtc
       61 gtccgacggc tcctcgctcg tcttcatggg cggcggcgcc ttcaagaaga agatcctccg
      121 cgtccacgcc tccggagggg aagcgcagga gttcgcgatc cgccccccga agggagtggc
      181 agggtttcgc ttcatcctgt atccgacgct gtctccggat gggaaatgga tcctcttcca
      241 ggcggagcag tttcgtgtgc acgttgtccc gagctcgggc ggagaagcca agctcctctg
      301 ccgggggcgg cgtccggtct gggctccgga cggccgctcg gtcgtctact cgaacgcgga
      361 tccggggaag aactactcgc tctggaggat tccgttctcg ccggcgaagg gcgcagccga
      421 cgggcccgcc gaaccggtga ccgtcggtcg cggcgtcgac acccaggcca cgatctcgca
      481 tgacggaagg cagatcgcgt tcaccgcgct cgaacagacg ttcaacctcg agacgctgcc
      541 gttcgacgcc gagctcggac gggcgaccgg cgaggtgaag gtcctgacgt ccggcaacaa
      601 cgacatcgag tggaagagct tgtccccgga tggccggtcg gtcgtttacg gaaacgccgg
      661 ccgtgtctgg agactcgacc ccgggcaggc ggcgtatccg ctgacgagcg acccggcctt
      721 catcgacggt cagccccggt ggtctcccga cgggcggtcg atcatcttcg aacgggctcc
      781 cgcgggcgaa ccggggaatt tcagcatctg gctgatggcc gccgacggcg cgggcccgcg
      841 gaagctcctc gagaaggccc ggtcagggta ctggatgccg gacagccgcg ggatcaccta
      901 ctcctcgtta tccgatttct acctgtacca cttcgatctc gcgacgcgga ggtcccgccg
      961 ggttgccgac gaggagacgc gggcgccgct ccacctcgtg agtaacgacg gcaagtggat
     1021 cgtctaccaa tcgtcggccc agagctccat cacgattcac gcccatccga tcgatggcgg
     1081 ggagacgcgc gtcgtcgcct cgaccccggg ccagatgggg agcgggcatc ccatgctctc
     1141 cccctccggg aactggttct actaccaggt cgaccacaag aacgtgtatc ggctgcccgg
     1201 gccgtcgcag ggatggaaga aagcggagcc tcagaaggtg acgaactttc ccgagtccgg
     1261 cctcttcatc gaagacccgc gtctctcgat ggacgggaag cagctcatct actcgcgcgg
     1321 gaagatcacc ggagacctct ggctgctccg tttcgggaaa cccggatcga agtaggcgtc
     1381 aggctctcgc cgcggcttcc gtgcggctac ccccccgagt ggcgaggaac acgccgagaa
     1441 ggatcaacgc gccccccgcg agcgcccgag cgggaagcct ctcgccgaga aaggccgcgc
     1501 cgagcaggac ggcgatcaac gtatcgacca gcgggatcgt gctgacgacc gaaatcggaa
     1561 cgcgcttgat cagccagaag aggcctccga aagtgatcgc cgtgccgaag atcgcgaggt
     1621 aggcgagcga accgatcgcc ggcggagtcc atcgcgccgt cgcgccgcgc tcgacgagcg
     1681 ccgcgagggg caggaggaac gccgctccca ccagtgtctc cccccagacg ttcacgatcg
     1741 gatcgacgcg tccgagatgc ttcttgacga tgacgctcgc gaaggcggcc acgatcgccg
     1801 ccacgaacac gagcccgccg ccgcgcagga gcgttccgaa gtccgcctgc agcgcccgcc
     1861 agagggcagc tccttcgatg agcgccacac cggagagtcc cagcccggca gcggctatcg
     1921 tgcgcggcgt cagcggctcg tccgggagga ggaagtgcgc gaagacgccg gcgcagatcg
     1981 gaaacgtcgc gaagagcagg gcggccaggc cggactcgat ccactgctgc gcgacgaaga
     2041 tgagcccgta gaagacgccg atctggagga acccggcgag cgcgatgaac gccgcttccc
     2101 gcgcgctggg ccggcgatcg cggtgccgga acgcgaacgg cgcgagcacg aggcacgcga
     2161 gcgccatgcg caaggcggcg aagcgcaacg gcggaaggta ctcgagcccg accttgatcg
     2221 agagccaggt cgagccccag atcgcgcaga gaagaatgta cacgacgagc gtgcggccgc
     2281 gaaggggcga cgagatcctc ggcctcacgg cagcgaaacg ccttccacga gcgtcggata
     2341 ctcgagatac ggcaacagct ctctcttcat ctgcggataa gattccgaac gtgttcgaag
     2401 tccggagcga tccgaacctg tccgaagggg aacgttaccg cgatctcgaa gccgccctcg
     2461 tgtccctgat cgacggcgag acggatgcgc tcgcgaatct ggcgaacgcc gccgctctgc
     2521 tcgcccactc gctcgaggcg atcaactggt gcgggtttta cctcctccgg ggcgaggagc
     2581 tcgtgctcgg cccgtttcag ggaaaaccgg cgtgcatccg gatcccgctc ggccggggcg
     2641 tctgcgggag ggccgccctc gagcgggcga ccctcgtggt cccggacgtg agccgctttc
     2701 ccgaccacat cacgtgcgat cccgcttctc ggtccgagat cgtcgttccg atcgtcgagg
     2761 gcgacgtcct ccgtggagtt ctcgacgtcg acgcgccgga ggtcggccgg ttcgacagag
     2821 aggatctcgc cgggctcgag aggttcgtcg cggcgctcgc gccgcgcatc gagtgggaga
     2881 ggctctgaat gggacagaag caattcggcg ggaaggtcac ctggctcggg cacgcctgtt
     2941 ttctcatcga atccccgaag ggcgtccgta tcgcgatcga tccctggatc ggcaacaatc
     3001 ccagattccc gaaggcattc gagtggggtc ggcttgacgt gatcgccgcg actcacgggc
     3061 atttcgatca cttcggcgac gatgggctcg ctctcgcgaa gaagacgggc gcgacggtca
     3121 tcgcgatctt cgagctcgcg ctccactgcg gcgcgaacgg agtcgaaaag gtctcgggga
     3181 tgaacaaggg cggcagccag aaggtctcgg gcgtggagtt ccggatggtg aacgcgatcc
     3241 actcctcggg gacctcgggc gcgggcaagg agacgaactt ccccgcagat ccgtgcgggt
     3301 acgtcatcac gttcgaggac ggcttccgga tctaccacgc cggcgatacg aacgtcttcg
     3361 ccgacatggc gctctacggc gagctctacg agccggacct cgcgctgctg ccgatcggcg
     3421 acttctacac gatgggaccg agggaagccg cgaaggcgtg ccagctcctc ggcattccgc
     3481 gcgtcatccc gatacactgg ggcacgtttc cggcgctccc gggaaccccc gcggcgttcc
     3541 ggcgggaggt cgaccggcgc ggcctgccga cgcaggtgat cgagctttcg ccgggacaga
     3601 gctggccgta aaggagagat ccaatgagaa agccgatccg tttcctcccc acccttctgg
     3661 cgctcggtct cgccgtcgcg tgcgtgagca cccgcgccga ctggcagctc ctgggccgga
     3721 aggacgtttc gttcctggtc gagcgcgaca cgatcgacgt cgaacgatcc gaaggacgtc
     3781 ttcacgcgct gaagatcgtc gtcgagggcg ctcccgtcga gattcgcgat atccgggtga
     3841 ccttcggcga cggctcgacg ttccaccccg agacccgcct gacctttgcc gcggggagcg
     3901 agagccggtc aatcgacttg ccggggcgcg gacgcgtcat ccggaagatc gacttcgtct
     3961 accgcaaaac gtccggaatc ttccgccagg cgaccgtctc ggtctacggc cggtaaccga
     4021 gaccggaacc ctcgcgctcg cgccgcgagg gactacttgg gtgcggccgc cacagccggt
     4081 tggaggtcga acgctccgtt gctctcaacg aagaataggc attccgatgc ctggtcgcag
     4141 cttgtcgtgt gccggtagct tccgccaggc tgcatcaggt aggagccggg acccagtcgg
     4201 aactcgggct tcccctccgg gcgttgaatg tacgtgccag acacgatgac gaccttcatg
     4261 tcgtgagtgt gtgtgtgtag cggcgtcgcg aatcccgccg gtagtctgaa gaacgcgccg
     4321 aacgcgccct tctgatggct gccccacaaa tccgcgacct tggctcccgg agcgccagcg
     4381 gggtcgaggt cggtccaatg caggtccgca gccggcatcg gggtcggcga aatgacctcc
     4441 gccccctcaa cgctcttcgt accctgcgcc agaaccgcca gcgccaaaac gaaaaatgat
     4501 acgaacgaca ggacgtttct tgcgttcatc ctcatccggc cctccttatt tcgcttttcg
     4561 agagttgcgg cgatcacaag cgttcttctg gacacctccc agtggcatgc cgaagtgcgc
     4621 tcatgtggct cctccgatcc taactccagc gcactccgga gaaatctcga aattcaaccg
     4681 ccggaacggc gaacgccgag tggcgggagc gttggtttcg gctcgcggcg cgagtagccc
     4741 ggttcgcggc gcgaggagcc cggttcgcgg cgcgaggagc ccggttcgcg gcgcgaggag
     4801 cccggttcgc ggcgcgagg
//
LOCUS       DASWAV010000491         2310 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U2665
            k141_428863, whole genome shotgun sequence.
ACCESSION   DASWAV010000491 DASWAV010000000
VERSION     DASWAV010000491.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960316
            Sequence Read Archive: SRR10260007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2310)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2310)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:47:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,929
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2310
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_428863"
                     /isolate="SMAG_U2665"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.36444 N 132.31889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..240
                     /locus_tag="VF958_10240"
     CDS             <1..240
                     /locus_tag="VF958_10240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9149527.1"
                     /translation="PPADRHHGLLMRKVDYAPVIETTDGNASKVEVRVMFVWRDGRPF
                     PVTTLARLSQGPMMGVAFNKDRTWVGSSGCLWPAG"
     gene            306..932
                     /locus_tag="VF958_10245"
     CDS             306..932
                     /locus_tag="VF958_10245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9149528.1"
                     /translation="MRRIGTLLSIVLSVLFFSTLALAQEMKQETKTTTTTKKTEMKMK
                     MGTKPARSNAWKIQNAMSAAPRAISKDATIMDFPASEGAEMPVLRKGTNGWTCLPDMA
                     DTPANDPQCLDKMAMEWAAAWIGHKEPNLASAGLGYMLQGGGSASNTDPYATKPAAGE
                     MWMKEPPHLMIFPAGGKIDTTVYSSDPKSGPWVMWGGTPYQHLMVPVK"
     gene            complement(965..1405)
                     /locus_tag="VF958_10250"
     CDS             complement(965..1405)
                     /locus_tag="VF958_10250"
                     /inference="COORDINATES: protein motif:HMM:NF039496.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5655 domain-containing protein"
                     /protein_id="HEX9149529.1"
                     /translation="MTPTRKATAARQSWACPACAREFPKPNQWHSCAVKALDEHFRGR
                     DPELREIFDELVRRLRTLGPIKVDPVKTSINLTARHHLGAVTVRGSFLRLGFLAEKRI
                     EDGRIVHFERLGPAKFGHSVILESIEDLDPVVMGWLAAAYRLRS"
     gene            complement(1402..2115)
                     /locus_tag="VF958_10255"
     CDS             complement(1402..2115)
                     /locus_tag="VF958_10255"
                     /inference="COORDINATES: protein motif:HMM:NF012958.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase-fold protein"
                     /protein_id="HEX9149530.1"
                     /translation="MNREYVVWDSPTLQRRMELLWYGHAGRPMIWFPTSYAHFYENED
                     FGLIGAVGDLIGGGRIQVACVDGIDEEALYAREKHPAERLARYDQYDAYLYREVVPWI
                     AAKTSHAPKITTLGASFGAYHAVNFAFRHPDVVDKTIGFSGKYDIHSFLDGYWNDTAY
                     FHCPTAYVPNMNGEWVERLSQMEICIVTGATDNILSGTTDMIRILREKGIPLRGDVWD
                     APYGHDWPWWKIQIRNYVP"
     gene            2178..2252
                     /locus_tag="VF958_10260"
     tRNA            2178..2252
                     /locus_tag="VF958_10260"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2210..2212,aa:Pro,seq:tgg)
BASE COUNT          413 a          720 c          786 g          391 t
ORIGIN      
        1 ccgccggcgg atcggcacca cggactgctc atgcgcaagg tcgactacgc gcccgtcatc
       61 gagaccaccg acggaaacgc gtcgaaagtc gaggtgcggg tcatgttcgt ctggcgcgac
      121 ggaaggccgt tcccggtgac gacgctcgca cgcctctcgc agggcccgat gatgggcgtc
      181 gccttcaaca aggaccggac ctgggtcggc tcgagcgggt gcctctggcc cgccggttga
      241 gcgggcattc atccctcatt catcgccgca ccctcacgct ggaggcgaac gaaaggagga
      301 ttctcatgag gcgaatcgga actctcctct ccatcgtcct gtccgtgctc ttcttctcca
      361 cgctggcgct cgcgcaggag atgaagcagg agacgaagac gacgaccacg acgaagaaga
      421 ccgagatgaa gatgaagatg gggacgaagc ccgcgcgctc gaatgcgtgg aagatccaga
      481 acgcgatgag cgccgcgccc cgggcgatct ccaaggacgc gacgatcatg gactttccag
      541 cgagcgaagg ggccgagatg ccggttctcc ggaagggcac gaacggctgg acctgtctcc
      601 ccgatatggc cgacaccccc gcgaacgatc cccagtgcct ggacaagatg gccatggagt
      661 gggcggcggc atggatcggt cacaaggagc cgaacctcgc gtccgccggc ctcggttaca
      721 tgctccaggg cggcggcagc gccagcaaca cggaccccta cgcgacgaag ccggcggcgg
      781 gcgagatgtg gatgaaggag ccgccgcacc tcatgatctt tccggcgggc ggcaagatcg
      841 atacgacggt ctatagcagc gatccgaagt ccgggccgtg ggtcatgtgg ggagggactc
      901 cgtaccagca cctgatggtg ccggtgaagt aatcccgagt tgggcgcggg ccgccgcggc
      961 ccgcctaaga ccgcaggcgg taggccgcgg cgagccagcc catgacgacg ggatcgaggt
     1021 cttcgattga ctcgaggatg accgagtgcc cgaacttcgc cgggccgaga cgttcgaagt
     1081 gaacgatcct cccgtcctcg atcctcttct ccgccaggaa ccccaggcgc agaaagctcc
     1141 cgcgtaccgt gacggcgccg agatgatgac gcgcggtcag gttgatcgag gtcttgaccg
     1201 gatcgacctt aatcgggccg agggtccgca accgtcggac gagctcgtcg aagatctcgc
     1261 gcagctcggg gtctctgccg cgaaagtgct cgtcgagcgc tttcaccgca caggagtgcc
     1321 actgattcgg cttggggaac tcgcgcgcgc aggcggggca cgcccaggac tggcgcgcgg
     1381 cggtggcctt tcgggttgga gtcacgggac gtagttccgg atctgaatct tccaccacgg
     1441 ccagtcgtgt ccgtagggcg cgtcccagac gtcgccgcgg agcgggattc ccttttcgcg
     1501 caggatgcgg atcatgtccg tcgtgcccga gagaatgttg tccgtcgcgc cggtgacgat
     1561 gcagatctcc atctgcgaga ggcgctcgac ccactcgccg ttcatgttgg gaacgtaggc
     1621 cgtcgggcag tggaagtagg cggtgtcgtt ccagtagccg tcgaggaacg agtggatgtc
     1681 gtacttgccc gagaacccga tcgtcttgtc gacgacgtcg ggatggcgga aggcgaagtt
     1741 gacggcgtgg taggcgccaa aagaagcgcc gagcgtcgtg atcttcgggg cgtgactcgt
     1801 cttcgcggcg atccacggaa cgacctcgcg gtagagatac gcgtcgtact ggtcgtaccg
     1861 cgcgaggcgc tcggccgggt gcttttcgcg cgcgtagagt gcctcctcgt cgattccgtc
     1921 cacgcacgcg acttggattc gccctccacc gatgaggtcg ccgaccgcgc cgatgaggcc
     1981 gaagtcctcg ttctcgtaga agtgagcgta cgacgtcggg aaccagatca tcggccggcc
     2041 ggcgtggccg taccagagga gctccattcg gcgctgaagc gtgggcgaat cccagacgac
     2101 gtactcgcgg ttcatggcgt gatccacgca tccgcccgcg gattcgccgc ggtgccgcgt
     2161 gggataattc ctccttccgg ggcgtagcgc agcggtagcg tatctgcttt gggagcagat
     2221 ggtcgccggt tcgatcccgg ccgccccgac cagtcttttc cgggtcgttt tctcgccgga
     2281 cccgaatcga tctcggttcg acaaggagga
//
LOCUS       DASWAV010000492         5153 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U2665
            k141_346577, whole genome shotgun sequence.
ACCESSION   DASWAV010000492 DASWAV010000000
VERSION     DASWAV010000492.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960316
            Sequence Read Archive: SRR10260007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 5153)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5153)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:47:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,929
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5153
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_346577"
                     /isolate="SMAG_U2665"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.36444 N 132.31889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..758)
                     /locus_tag="VF958_10265"
     CDS             complement(<1..758)
                     /locus_tag="VF958_10265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011689269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX9149531.1"
                     /translation="MKTVIRGGTVVTATDLYRGDVLIEDERIAAVGTSFGDVAADRTI
                     DASDKHVIPGGIDVHTHLDMPFGGTTSADDFESGTIAAAHGGTTSIVDFAIQYRGQTL
                     RHALDTWMAKADGKSAIDYGFHMIVTDLNEAVESEMDALVREGVTSFKLFMAYPGVFM
                     LDDASIFRALLRTGENGGTICMHAENGGVIDVLVKRALAGGKAAPKYHALTRPARAEG
                     EATHRAIALAEIAGVPIYIVHLSAAEALEMVTEAR"
     gene            complement(755..1627)
                     /locus_tag="VF958_10270"
     CDS             complement(755..1627)
                     /locus_tag="VF958_10270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012791089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrilase-related carbon-nitrogen hydrolase"
                     /protein_id="HEX9149532.1"
                     /translation="MPRKVTGGLIQCSTPTSDPSLPIERIKEDAIARHIPFIEEAGKK
                     GVQILGLQEVFNGPYFCPSQDPHWYDMAEPVPGPTTELMAEYAKKYRMAMVVPVYERE
                     QAGVYYNTAAVYDADGTYLGKYRKNHIPHTSGFWEKYFFKPGNLGYPVFRTRYAKVGV
                     YICYDRHFPEGARLLGLNGAEIVFNPSATVKGLSQYLWKLEQPAHAVANGYFMACSNR
                     VGVEAPWNIGQFYGSSYFVDPRGNFLAVGSEDKDELVVAEMDLDLIEEVRRVWQFFRD
                     RRPETYQPMAEITA"
     gene            complement(1704..3209)
                     /locus_tag="VF958_10275"
     CDS             complement(1704..3209)
                     /locus_tag="VF958_10275"
                     /EC_number="1.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011522337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018478 - malonate-semialdehyde
                     dehydrogenase (acetylating) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-acylating methylmalonate-semialdehyde
                     dehydrogenase"
                     /protein_id="HEX9149533.1"
                     /translation="MTDSAVADRAGTRASSSNEVEVLRNFVGGRWVESHADEFLDVYN
                     PAKGEVIARTPLSTAEDLDAAVVAAKKAFPEWRDTPAVVRSRALFRFRQLLEDNFEEL
                     ARAVTTEHGKTIDESRGSVRRGIECVEVACGTPSLMMGYALENIASGVDCAVIRQPVG
                     VCAAIAPFNFPAMVPLWFLPFAVATGNTIIVKPSEQVPLTQRLIFRLLEKCDLPPGVV
                     SLVNGGREVVEAICDHPEIRAVSFVGSTPVARTVWRRATQAGKRVQALGGAKNFIVVM
                     PDADWGRSISAITESFFGCAGERCLAGSVLVPVGEAHHEARDRMAEAAKALKVGDGME
                     KGVQMGPVISARHREKVLGYIDKGVDEGAKLVVDGRKVRVEGREEGFFLGPTLFDDVK
                     PSMAIGHEEIFGPVAAITPVKSLEQAIEVMLAHPNANATSIFTSSGKAAREFARLASA
                     SMVGVNIGVAAPMAYFPFGGAKDSFFGDLKVHGRDAYEFYTDKKVVISRWF"
     gene            3478..3789
                     /locus_tag="VF958_10280"
     CDS             3478..3789
                     /locus_tag="VF958_10280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015248514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HEX9149534.1"
                     /translation="MKLYVGNLPYTMDEGDLRDLFTPYGTPSSVAVIMDRGTGRSKGF
                     GFVEFENDTEAKAAISALNGRDFNGRALTVSEARPRAEGGGGGGGRGGYGGGRGGGGR
                     Y"
     gene            3831..4946
                     /locus_tag="VF958_10285"
     CDS             3831..4946
                     /locus_tag="VF958_10285"
                     /EC_number="1.14.19.-"
                     /inference="COORDINATES: protein motif:HMM:NF012699.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid desaturase"
                     /protein_id="HEX9149535.1"
                     /translation="MTNSARQTEKDWVNIFFLSLTPVIGVVGTAAYAIRFGVQWWEPA
                     LFLVLYAFVGLSVTAGYHRLYAHKGYVAHPLVHAFFVFFGAMALQNSVLKWASDHRTH
                     HKYVDRDWDPYSIKRGGLWAHILWLFYKRPPHETFDNVPDLKKNRLVMLQYRFSNALG
                     IVAGLGLPTIIGWAFGSPLGGLLWGGFLRIVVIHHTTFFVNSIAHLYGSRPYTDENSA
                     RDNWMLAFVTNGEGYHNFHHRFPSDYRNGIRWYHWDPTKWLIRALAGMHLARGLRETP
                     WSVVEKARLEMAARRAESRLANLSADVRELVRVRLEEAGRFLERAAAQRARAQARMSV
                     SVGSWKRALRLERAYVFRARRYRRGALRLLSTATQGA"
     gene            4973..>5153
                     /locus_tag="VF958_10290"
     CDS             4973..>5153
                     /locus_tag="VF958_10290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006357402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0019239 - deaminase activity [Evidence
                     IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deaminase"
                     /protein_id="HEX9149536.1"
                     /translation="MLDVAVEEARQGLREGGIPIGAALFAREGRLLGRGHNRRVQDGD
                     PSAHGETDAFRRAGRQ"
BASE COUNT          847 a         1680 c         1713 g          913 t
ORIGIN      
        1 cgcgcctccg tgaccatctc gagcgcctcg gccgccgaga ggtggacgat gtagatggga
       61 actcccgcga tttccgcgag tgcgatcgcg cggtgcgtgg cctcgccctc ggcgcgcgca
      121 ggccgtgtca acgcgtggta cttcggcgcg gcctttccgc cggcgagcgc ccgcttcacg
      181 aggacgtcga tgacgccgcc gttctcggcg tgcatgcaga tcgtgccgcc gttctcgccc
      241 gttcgcagga gcgcccggaa gatcgacgcg tcgtcgagca tgaagacgcc cgggtaggcc
      301 atgaagagct tgaaggaggt cacgccttcg cgcacgagcg cgtccatctc gctctcgacg
      361 gcctcgttca gatcggtgac gatcatgtgg aagccgtagt cgatcgcgga cttcccgtcg
      421 gccttcgcca tccaggtgtc gagcgcgtgc cggagggtct gcccgcggta ctggattgcg
      481 aagtccacga tcgaggtcgt cccgccgtgt gccgcggcga tcgtgccgga ctcgaaatcg
      541 tcggcggacg tcgttccgcc gaacggcatg tcgaggtggg tgtggacgtc gatgccgccg
      601 gggatgacgt gcttgtcgga agcgtcgatg gtccgatccg cggccacgtc cccgaacgag
      661 gtgccgaccg ccgcgatcct ctcgtcctcg atcagcacgt cgccgcggta gagatcggtc
      721 gcggtgacga ccgtgcctcc ccggatcacc gtcttcaagc ggtgatctcg gccatcggct
      781 ggtaggtctc gggtcgccgg tcgcggaaga actgccacac gcgacggact tcttcgatca
      841 agtcgaggtc catctcggcg acgacgagct cgtccttgtc ctccgagccg acggcgagga
      901 agttgccgcg cgggtcgacg aagtacgacg agccgtagaa ctgtccgatg ttccacggag
      961 cctcgacgcc gacccggttg ctgcacgcca tgaagtagcc gttcgcgacg gcgtgcgcgg
     1021 gctgttcgag cttccagaga tactgcgaca gtcccttcac cgtcgcggaa gggttgaaga
     1081 cgatctccgc gccattcaga ccgaggagcc gcgcgccttc cgggaagtgc cggtcgtagc
     1141 agatgtagac gcccactttc gcgtagcgcg tccggaagac gggatagccg aggttgcccg
     1201 gcttgaagaa gtacttctcc cagaaaccgg aagtgtgggg gatgtggttc ttgcgatact
     1261 tgcccaggta cgttccatcc gcgtcgtaga cggcggcggt gttgtagtag acgccggcct
     1321 gctcccgttc gtacaccggc acgaccatcg ccatccggta cttcttcgcg tattcggcca
     1381 tgagctcggt ggtcggcccg ggcaccggct cggccatgtc gtaccagtgc gggtcctgcg
     1441 aggggcagaa gtaaggtccg ttgaagactt cctggagtcc gaggatctgg acgcccttct
     1501 tgcccgcctc ttcgatgaac gggatgtggc gcgcgatcgc gtcctccttg atgcgctcga
     1561 tcgggaggga cgggtcagac gtgggcgtcg aacactggat cagccctccg gtcactttgc
     1621 ggggcatcag actgggtccg cggcggtcgc tgcgcgaccg gcggacagaa agtttaaaac
     1681 cagagcttgc ggtttcggct tgttcaaaac catctgctga tcacgacctt tttgtcggta
     1741 tagaactcgt acgcgtcccg cccgtgcacc ttcaggtccc cgaagaagct gtccttggcg
     1801 ccgccgaacg ggaagtacgc catcggcgcg gcgacgccga tgttcacgcc caccatcgac
     1861 gccgacgcca gccgcgcgaa ctcgcgcgcg gccttgccgc tcgacgtgaa aatcgacgtc
     1921 gcgttggcat tggggtgagc gagcatgacc tcgatcgcct gctccaggct cttcaccggc
     1981 gtgatcgcgg cgacgggacc gaagatctcc tcgtgcccga tcgccatcga aggcttcacg
     2041 tcgtcgaaga gggtcgggcc gaggaagaac ccctcctcac gtccctcgac ccggaccttg
     2101 cggccgtcga cgacgagttt cgccccctcg tccacgccct tgtcgatgta ccccaggacc
     2161 ttctcgcgat gacgggcgct gatcacgggc cccatttgaa cgcccttctc catcccgtcg
     2221 ccgaccttga gggccttcgc ggcctccgcc atccggtcac gcgcttcgtg gtgggcctcc
     2281 ccgaccggga cgagcacgct gccggcgagg cagcgctctc ccgcgcatcc gaaaaaggac
     2341 tcggtgatgg cggagatcga acgaccccag tcggcgtcgg gcattacgac gatgaaattc
     2401 ttcgcgccgc ccagcgcctg gacccgcttg cccgcctggg tggcacgccg ccacaccgtc
     2461 cgagcgaccg gcgtcgagcc gacgaaggag actgcgcgga tctcggggtg atcacagatt
     2521 gcctcgacga cttcccggcc tccgttgacc aggctcacga ctcccggcgg cagatcacac
     2581 ttttcgagca gccggaagat cagtcgctgc gtgagcggga cctgctcgga gggcttgacg
     2641 atgatggtgt tgccggtcgc aacggcaaag ggcaggaacc agagcggcac catcgccggg
     2701 aaattgaatg gcgcgatggc ggcacaaacg ccgacgggct gccggatgac ggcgcaatcg
     2761 accccggagg cgatgttttc gagcgcataa cccatcatga gggagggcgt tccgcaggca
     2821 acttcgacgc attcgatgcc ccgccggacg ctgccgcgcg actcgtcgat cgtcttgccg
     2881 tgttcggtcg tgacggcgcg cgcgagctcc tcgaagttgt cctcgaggag ctgccggaat
     2941 cgaaacaacg ctctcgaacg gacgaccgcc ggggtatcgc gccactcggg aaaagccttt
     3001 ttcgcggcga cgaccgcggc gtcgaggtcc tcggccgtcg aaagcggcgt gcgggcgatc
     3061 acttccccct tcgccgggtt gtagacgtcg agaaactcgt cggcgtgcga ttccacccag
     3121 cgaccgccga cgaaattgcg caggacctcg acttcgttcg aagaagaagc tcgggtgccg
     3181 gcccgatcgg ccacggcgga atcggtcatg gcagcctcct tttccgaaaa gaccgcgtcg
     3241 caggaagacg cagatagtag tcccgcatgt aaggcgcccg caaggcattg gacgctagga
     3301 acttgcggcc tcctgcgact gtgctaacct ttctcggttc tcgttccgct ccgttctgcc
     3361 agacctgctc tgacgaattc cgaacgataa ggtaacggga aggcgcggcg cgcgcggcgg
     3421 cggtcgcgtt cggacaacag ccggaatctg tcggcctgtc cggcaggagg aattcggatg
     3481 aaactgtacg tcggcaatct cccttacacg atggacgagg gcgacctccg cgacctcttc
     3541 accccctacg gaacgccgag ctcggtcgcg gtcatcatgg atcgcggaac cggtcgctcg
     3601 aagggcttcg gcttcgtcga gttcgaaaac gacaccgagg cgaaggcggc gatttcggcg
     3661 ctgaatggcc gcgacttcaa cggccgcgcg ctgactgtgt cggaggcgcg gccgagggcg
     3721 gaaggtggcg gcggcggcgg cgggcgcggt ggatacggcg gcggccgggg cggcggcggg
     3781 cggtattgag ttcgccaggg tcctcgaacc gcgagaatgg agaagctgtc gtgactaact
     3841 cagcgcgtca gacggagaag gactgggtca acatcttttt cctgtcgctc acgccagtga
     3901 tcggcgtcgt cggcacggcg gcctacgcca tccgattcgg agtgcagtgg tgggagcccg
     3961 cactcttcct cgtactctat gcgttcgtcg gtctctcggt caccgcggga tatcaccgcc
     4021 tgtacgcgca caagggatac gtcgcccatc cgctggtgca cgctttcttc gtctttttcg
     4081 gtgcgatggc tctccagaac tcggtcctga agtgggcgtc ggatcaccgg acgcaccaca
     4141 agtacgtcga tcgggactgg gatccttaca gcatcaagcg cggcggactc tgggcccaca
     4201 tcctgtggct cttctacaag cggccgcccc acgagacgtt cgacaacgtc ccggatctga
     4261 agaaaaaccg cctcgtgatg ttgcagtacc gcttctccaa cgcgctcgga atcgtggcgg
     4321 gcctcggcct tccgacgatc atcggctggg cgttcggcag cccgttgggc ggtctcctgt
     4381 ggggaggttt tctccggatc gtcgtcatcc accacacgac cttcttcgtg aactcgatcg
     4441 cgcacctcta cggctcgcgg ccgtacacgg acgaaaactc cgcgcgggac aactggatgc
     4501 tcgccttcgt cacgaacggg gaggggtacc acaacttcca tcaccgcttt ccttcggatt
     4561 accgcaacgg catccgctgg tatcactggg atccgacgaa atggctcatt cgcgcgctcg
     4621 ccgggatgca cctggcgcga gggcttcgtg agaccccctg gtccgtcgtc gagaaggcgc
     4681 gcctggaaat ggcggcgcga cgggccgaga gccgtctcgc gaatttgagt gccgacgtgc
     4741 gcgagctcgt gcgtgtccgg ctcgaggagg ccggccgctt tctcgagcgg gcggcggcgc
     4801 agcgcgcgag agcgcaagcc cggatgtcgg tgtcggtcgg gtcgtggaag cgagcgctgc
     4861 gcctcgagcg cgcgtacgtc tttcgcgcgc gccgctatcg ccgcggcgcc ctgcgcctgc
     4921 tctccaccgc cacccaaggg gcgtgagccg gaccttctcc gattcccggt caatgctcga
     4981 cgtcgccgtt gaggaggcgc gccagggact gagggagggg gggatcccga tcggcgcggc
     5041 gctattcgct cgggagggac gcctcctcgg gcgaggccac aaccggcgcg tgcaggacgg
     5101 cgacccgtcc gcgcacggcg agacggacgc gttccgcagg gcggggcgcc agc
//
LOCUS       DASWAV010000493         2990 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U2665
            k141_346679, whole genome shotgun sequence.
ACCESSION   DASWAV010000493 DASWAV010000000
VERSION     DASWAV010000493.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960316
            Sequence Read Archive: SRR10260007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2990)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2990)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:47:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,929
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2990
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_346679"
                     /isolate="SMAG_U2665"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.36444 N 132.31889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            129..839
                     /gene="purQ"
                     /locus_tag="VF958_10295"
     CDS             129..839
                     /gene="purQ"
                     /locus_tag="VF958_10295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012844795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurQ"
                     /protein_id="HEX9149537.1"
                     /translation="MTGDKPKFGIVVFPGSNCDQDCHYVATELLGCDADYVWHRDTDL
                     KGADIVILPGGFAYGDYLRAGALARFSPVMETVIRFAMAGGPVLGICNGFQVLLEAGL
                     LPGAVRVNRGLRYVCKDVLMRVENASTAFTRLYEPGQIVRMPIGHGEGNYTAPPEMLT
                     ELEHEGGVVFRYCDEEGRVTDEANPNGATAGIAGIANREGNVVGLMPHPDRCAEGLLG
                     NDVGRKMFESAVAALAPA"
     gene            836..>2990
                     /gene="purL"
                     /locus_tag="VF958_10300"
     CDS             836..>2990
                     /gene="purL"
                     /locus_tag="VF958_10300"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011423260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurL"
                     /protein_id="HEX9149538.1"
                     /translation="MTPGGITAELIAEHNLSEEEYSRILEILGREPNIVELGVFSAMW
                     SEHCSYKSSRIHLKKLPTSGPRVIQGPGENAGIVDVGDGLAVAFKMESHNHPSFIEPY
                     QGAATGVGGILRDIFTMGARPIASLDSLRFGHLDAPRMRHLVDGVVRGIAGYGNCMGI
                     PTVGGETTFHPAYDRNILVNVMNVGVVERDRIFRGRAEGSGNPVIYLGSKTGRDGIHG
                     ATMASEEFAGDETRQKRPTVQVGDPFTEKLLMEACLELFRTDAVVGIQDMGAAGLTSS
                     SFEMAGRSGSGLEIDLSKVPVREPGMTPYEILLSESQERMLLVARKDRLETALRVFRK
                     WELEAVEIGRVTDSGRVVLFFDGAIVADLPAGPLSERAPLYDRPHAPPESVGSRGWET
                     LAEPGSYGACLRKILGADNVSQKSWIWTQYDHMVGTNTVVRPGGDAAVVRIKGTKKAI
                     AMKSDVNPFFCACDPYRGAAIAVAEAARSIAATGARPLAITDCLNFGNPERPEIMGQF
                     EAAIRGLSDACRALDVPVVSGNVSFYNETDGRSIPPTPTVGMIGLLEDVSRIVRVAFR
                     RAGDVVALAGETRDELGASEFLRTVHGRDDGPCPEVDLAAERRLVDLLVRLAGEGRLA
                     SAHDVSDGGLAVALAECAMDSGLGVDVRLDSSVRPSSLLFGESTGRAILTFAPEEERA
                     VRSAAAAGGVPLQLVGRVSGDRLRIDGGRVLVDESV"
BASE COUNT          458 a          991 c         1068 g          473 t
ORIGIN      
        1 aaggtcttct tcatcgacgt ggcgggaacg gatccggtcg ccgtcgaggc ggaggcgcga
       61 aagatggcgg aggagctcct cgcgaacacc gtgatcgagg ggtatgacgt acgcatcgcg
      121 ggggcgccgt gaccggcgac aagccgaagt tcggaatcgt cgtgtttccc ggctccaact
      181 gcgaccagga ctgccactac gtcgcgaccg agctcctcgg gtgcgacgcc gactacgtct
      241 ggcaccgtga cacggatttg aagggcgccg acatcgttat cctccccggc gggttcgcgt
      301 acggcgacta cctgcgggcc ggcgcgctcg cgaggttctc gccggtcatg gagaccgtca
      361 tccgcttcgc gatggccggg gggccggtgc tcggcatctg caacggcttc caggtgcttc
      421 tcgaagcggg tctgctccct ggcgcggttc gcgtgaaccg cggcctccga tacgtctgca
      481 aggacgtgct catgcgggtg gagaacgctt cgacggcctt cacccggctc tacgagcccg
      541 ggcagatcgt gaggatgccg atcggtcacg gcgagggcaa ttacacggcg ccgccggaga
      601 tgctgacgga gctcgagcac gaggggggcg tggtcttccg ctactgcgac gaagaggggc
      661 gcgtgaccga cgaagcgaat ccaaacggcg cgacggccgg catcgccggc atcgccaacc
      721 gcgagggcaa cgttgtcggc ctcatgccgc accccgaccg ctgcgcggag ggcctcctcg
      781 gcaacgacgt ggggcgcaag atgttcgagt cggccgtcgc cgcactcgcg ccggcatgac
      841 cccgggagga atcaccgccg agctgatcgc ggagcacaac ctctcggaag aagagtactc
      901 gcgcatcctc gagatcctcg gccgcgagcc gaacatcgtc gagctcggcg tcttctccgc
      961 gatgtggagc gagcactgtt cgtacaaatc ctcgcgaatc cacctgaaga agctgccgac
     1021 gagtggtccc cgcgtgatcc aggggcccgg cgagaacgcc ggcatcgtgg acgtcgggga
     1081 tggcctcgct gtcgctttca agatggagtc gcacaaccac ccgtcgttca tcgagcctta
     1141 ccagggcgcg gcgaccggcg tcggcgggat tctccgcgac atcttcacca tgggggcgcg
     1201 gccgatcgcg tcgctcgact cgctccgctt cggtcacctg gatgcgccgc ggatgcggca
     1261 tctcgtggac ggcgtcgtgc gcgggatcgc gggttacggc aactgcatgg ggattccgac
     1321 ggtgggcggc gagaccacct tccacccggc gtacgaccgc aacatcctgg tcaacgtcat
     1381 gaacgtgggt gtcgtcgaac gtgaccggat tttccgcggc cgcgccgagg gttcgggcaa
     1441 tcccgtcatt tacctcggct cgaagaccgg ccgcgacggg atccacggcg ccacaatggc
     1501 ctccgaggag ttcgccggag acgagacgcg gcagaagcgc ccgacggtgc aggtcggaga
     1561 cccgttcacc gagaagctcc tgatggaggc ctgcctggag ctcttccgga cggacgcggt
     1621 cgtcggcatc caggacatgg gcgcggcggg actcacgtcc tcgtccttcg agatggcggg
     1681 ccggtcgggc agcgggctcg agatcgatct ctcgaaggtg cccgtccggg agcctgggat
     1741 gacgccgtac gagatcctcc tctcggagtc gcaggagagg atgctgctcg tcgcgcgcaa
     1801 ggaccggctc gagaccgcgc tgcgggtctt ccgcaagtgg gagctcgagg cggtcgagat
     1861 cgggcgggtg accgacagcg gacgtgtcgt cctcttcttc gacggggcga tcgtggcgga
     1921 cctgcctgcc gggcccctct ccgagcgcgc gccgctgtac gaccgcccgc acgcgccccc
     1981 ggagtccgtc ggatcgcgcg gctgggagac gctcgcggag cccgggagct acggcgcctg
     2041 cctccgaaaa atcctcgggg cggataacgt ctcgcagaaa tcctggatct ggacgcaata
     2101 cgaccacatg gtcggcacga acacggtcgt ccggccaggc ggcgacgcgg cggtcgtgag
     2161 gatcaagggg acgaagaaag cgatcgcgat gaagagcgac gtcaacccgt tcttctgcgc
     2221 ctgcgatccg taccgcgggg cggcgatcgc ggtcgccgag gcggcgcgtt cgatcgcggc
     2281 gaccggcgcc cgccccctcg ccatcaccga ctgcctgaac ttcggaaacc cggagcgccc
     2341 cgagatcatg ggtcagttcg aggcggcgat ccgcgggctc tccgatgcgt gccgggctct
     2401 cgacgtgccg gtcgtctccg gcaacgtctc cttctacaac gagaccgacg gccgctcgat
     2461 ccctccgacg ccgaccgtcg gaatgatcgg tctcctggag gacgtgtccc ggatcgtgcg
     2521 ggtcgccttc cgccgcgcag gcgacgtggt cgcgctcgcg ggggagacgc gcgacgagct
     2581 cggcgcgtcg gagttcctga ggacggtcca cgggcgcgac gacggtccgt gtcccgaggt
     2641 cgatctcgcc gcggagcgtc gcctggtcga ccttctcgtt cgactcgcgg gggagggccg
     2701 gctcgcctcg gcgcacgacg tctcggacgg cggcctcgcg gtggcgctcg cggagtgcgc
     2761 gatggactcg gggctcggcg tggacgtgcg tctcgattcg tccgtgcgtc cgtcctctct
     2821 gctctttgga gaatcgaccg gacgcgcaat cctcacgttc gccccggagg aagagcgagc
     2881 cgtgcggtcg gcggccgcgg cggggggagt tccgttgcag ctcgtcggcc gcgtgagcgg
     2941 agaccgcctt cggatcgacg gcggccgcgt tctcgtggac gagtccgtcg
//
LOCUS       DASWAV010000494         2686 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U2665
            k141_268060, whole genome shotgun sequence.
ACCESSION   DASWAV010000494 DASWAV010000000
VERSION     DASWAV010000494.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960316
            Sequence Read Archive: SRR10260007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2686)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2686)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:47:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,929
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2686
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_268060"
                     /isolate="SMAG_U2665"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.36444 N 132.31889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(64..1551)
                     /locus_tag="VF958_10305"
     CDS             complement(64..1551)
                     /locus_tag="VF958_10305"
                     /inference="COORDINATES: protein motif:HMM:NF024758.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HEX9149539.1"
                     /translation="MSKRRSLTLIAVACLFSVALFAAPVPVRLSVESQTHVSDVSDLA
                     MQKVELHRSLVARESASGIAKKLEVKLTPEEKAAAEAREDEGRLRVGIEKSVGVVVRP
                     VRSPRFGDVAAADGSILWTGRVTASGASGIRLHLSPFDLPESAELYVYAADGQVYGPY
                     TGRGPVDDGELWTNTILGDEVRLQLSVSPEDAARTRLVIASIGYLGPGLPMAGSNLCS
                     DNAACVENAACWNVSAVANAHDAVASILFASGGFYYICSGGLISDANPSNLPYFLTAN
                     HCISRSGEASSMETYFDYESGSCTSPNCTQPYNNAGDTVGSAIVASNRTGDYTLLQLS
                     STPRTKDGVVTYLGWTATAVANTNGAHLYRLSHPSGAPQAYSQHDVDTSKPTCSSWPR
                     GSWVYSRDVTGATEGGSSGSPVLNASGQIVGQLSGGCGFNVNDVCDASSNATVDGAFA
                     SYYGQVSSILGGGGPSCSAAGALCSMDSQCCSNSCKGKPGAKKCK"
     gene            complement(1644..2588)
                     /locus_tag="VF958_10310"
     CDS             complement(1644..2588)
                     /locus_tag="VF958_10310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015457207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEX9149540.1"
                     /translation="METRNIGSLKVSAVGLGGNNFGGRLDVEGTRAVIDAALDAGINF
                     IDTADIYGGGKSEKFLGEALGSRRKDVVLATKFGMEVDRRRRGARPAYVRQACEDSLR
                     RLHTSWIDLYQLHQPDPETPIADTLGTLADLVRHGKVRQIGCSNFSAEQIREASAAAR
                     PGGATFVSVQNEYSLLHREPEREVLPECERRGLAFIPYFPLASGVLTGKYSSGGSPPD
                     ARLSPGSRFRDRFLNDRNLAIAKRLKEFATSQGHTLLELAFSWLLAHGAVASVIAGAT
                     KPDQVRANVNAAAWKLTPEDLAEIDRISSGSGNLARTG"
BASE COUNT          429 a          868 c          915 g          474 t
ORIGIN      
        1 aaagtaaacg cctgaaaaaa gaggagcccg aaggctcctc ctggttcgac gaagatgcgg
       61 gccttactta cacttcttcg cgccgggctt gcccttgcag ctgttggaac agcactgcga
      121 atccatgctg cagagcgcgc ccgcggcgga gcacgacggc ccgccgccgc ccaggatcga
      181 tgagacctgg ccgtaatagc tcgcgaaggc gccgtccacg gtcgcgttcg agctcgcgtc
      241 gcagacgtca ttgacgttga agccgcatcc gcccgacaac tgtccgacga tctggccgga
      301 cgcgttcagc acgggagagc cgctgctgcc gccttcggtc gcgcccgtga cgtcgcggct
      361 gtaaacccag ctgccgcgcg gccacgaact gcacgtcggt ttcgacgtgt cgacgtcgtg
      421 ctgcgagtat gcctgcggcg ctccggaggg atggctcagg cggtagaggt gtgcaccatt
      481 ggtgttcgcg accgcggtcg ccgtccagcc gaggtacgtc acgacgccgt ctttggtcct
      541 gggggtcgaa gacagctgca gcagcgtgta gtcgcccgtc cggttgctcg ccacgatcgc
      601 cgatccgacc gtgtcgccgg cgttgttgta cggctgcgtg cagttcgggc tcgtacagct
      661 tcccgactcg tagtcgaagt acgtctccat gctggacgct tcgccgctcc gggagatgca
      721 gtggttcgcg gtcaggaagt acggcaggtt ggaggggttc gcgtcggaga tgagtccgcc
      781 gctgcagatg tagtaaaagc cgccggacgc gaagaggatg ctcgcgaccg cgtcgtgggc
      841 gttcgcgacc gcggagacgt tccagcaggc cgcattttcg acgcaggcgg cgttgtcgct
      901 gcagaggttg gaacccgcca tcggaagccc cgggcccagg tagccgatgc tcgcgatcac
      961 gagacgggtt cgcgccgcgt cttcgggaga gaccgagagc tgcagcctca cctcgtcgcc
     1021 gaggatcgtg ttcgtccaga gctcgccgtc gtcgaccggg ccgcggcccg tgtagggacc
     1081 gtaaacctgg ccgtcggcgg cgtagacgta gagctccgcg ctctcgggca ggtcgaaggg
     1141 ggagaggtgc agccggatgc cggaggcgcc ggacgccgtg acgcgtcctg tccagaggat
     1201 cgagccgtcg gcggccgcga cgtcgccgaa gcggggagac cgcacgggcc tcaccaccac
     1261 gccgacgctc ttctcgatcc caacgcgcag gcggccttcg tcttccctcg cctcggccgc
     1321 ggccttctct tccggcgtca acttcacctc gagcttcttc gcgattcccg acgcggattc
     1381 gcgtgcgacg agcgaacggt gcaactcgac cttctgcatc gcgagatccg agacgtcgga
     1441 gacgtgcgtc tggctctcga cggaaagacg cacggggacg ggcgccgcaa aaagcgcgac
     1501 ggagaacaga caagcgaccg cgatcaaagt caacgaccgg cgtttggaca tagtccctcc
     1561 atggtcagtg gcctggaagc cgttttgtgg atggaagcga agtatacccc gccgacgagg
     1621 gtcacagggg gacccaggag tccctagccg gtgcgcgcga ggtttccgct gccgctcgag
     1681 atgcggtcga tttccgcaag atcctcgggg gtcagcttcc aggctgcggc gttcacattt
     1741 gcccggacct gatcgggctt cgtcgccccg gcgatgaccg aggctacggc gccgtgcgcg
     1801 aggagccagg agaatgcgag ctcgaggagc gtgtgaccct gcgacgtcgc gaactctttg
     1861 aggcgcttcg cgatcgcgag gttccggtcg ttcaggaatc ggtcgcggaa ccggcttccc
     1921 ggagagaggc gcgcatccgg cggcgaaccc ccgctggagt actttcccgt cagcaccccg
     1981 ctcgcgagcg ggaagtacgg gatgaaggcg agcccgcgcc gttcgcactc gggcagcacc
     2041 tcgcgctcgg gttcgcggtg caggaggctg tactcgttct ggacactgac aaacgtcgcg
     2101 cctccgggtc gcgccgcggc gctcgcctcg cggatctgct cggcggagaa gttcgagcat
     2161 ccgatctgcc ggacttttcc gtgccggacg aggtcggcga gggttccgag cgtgtcggcg
     2221 atgggagtct ccgggtccgg ctgatgaagc tgatagagat cgatccaact ggtgtggagc
     2281 cggcgaaggc tgtcctcgca ggcctggcgc acgtacgctg gacgagcgcc ccgccgccgg
     2341 cggtcaacct ccatcccgaa cttcgtcgcg aggacgacgt cttttcggcg gcttccgagc
     2401 gcttctccca gaaacttttc gctcttgccg ccgccgtaga tgtcggcggt gtcgatgaaa
     2461 ttgatgccgg cgtcgagagc cgcatcgatg acggcacgcg ttccttcgac gtcgagccgg
     2521 ccgccgaagt tgttgccgcc gaggcccacg gcgctgactt tcagcgatcc gatgtttcgt
     2581 gtttccatat cgctggtcgc tgcagaatgt atgcctcgca gatcgttccg gacctcgcct
     2641 cgatcccggc cgatccgccg ttcttgctcg ggcggacccc ggtgac
//
LOCUS       DASWAV010000495         4068 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U2665
            k141_188999, whole genome shotgun sequence.
ACCESSION   DASWAV010000495 DASWAV010000000
VERSION     DASWAV010000495.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960316
            Sequence Read Archive: SRR10260007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 4068)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4068)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:47:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,929
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4068
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_188999"
                     /isolate="SMAG_U2665"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.36444 N 132.31889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            56..682
                     /locus_tag="VF958_10315"
     CDS             56..682
                     /locus_tag="VF958_10315"
                     /inference="COORDINATES: protein motif:HMM:NF024820.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX9149541.1"
                     /translation="MPDTVLTPPADLLRRPASIEEPPAPRRDTDPFDISDIPYKPPGV
                     PAVPISDRPDVFSRDVSLDSIISPKRESDGEIITKKIRLPEAMWPFEPPAPAGPAEAP
                     SPPPSPPSEPAPPEPTGRTLADLYFDQGHYPEALAIYRELLAADPANEDLARLRADAE
                     RLAAATPPRQLPPPDPGRQRRLEKIRVLNEWLDVIRTSAAADRDGRIR"
     gene            666..1028
                     /locus_tag="VF958_10320"
     CDS             666..1028
                     /locus_tag="VF958_10320"
                     /inference="COORDINATES: protein motif:HMM:NF015232.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="roadblock/LC7 domain-containing protein"
                     /protein_id="HEX9149542.1"
                     /translation="MGAFAESLARIARRVEGARAVSLIGADGIPVETWGSVEGLSFEV
                     LAAEMAGLLKSARSAGGAAGAGPLREVATVTDGAITILSRVTAEYYLLLLLGREGNLG
                     RGRFELRKAAADLEKELV"
     gene            1033..1503
                     /gene="accB"
                     /locus_tag="VF958_10325"
     CDS             1033..1503
                     /gene="accB"
                     /locus_tag="VF958_10325"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012811089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HEX9149543.1"
                     /translation="MLSFKEIKELIDLVSERGLAGVEIERAGFRLRIEGARPAAVTAP
                     AEAVPAPSPAAAAPAAAAPTAAAAEAARETEGIHVITSPIVGTFYRAPSPEADAFAEV
                     GERVARGKVLCIIESMKLMNEIESDVEGEIVAVYARNGQPVEYGEKLFGIRLAA"
     gene            1516..2859
                     /gene="accC"
                     /locus_tag="VF958_10330"
     CDS             1516..2859
                     /gene="accC"
                     /locus_tag="VF958_10330"
                     /EC_number="6.3.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009624072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0004075 - biotin carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HEX9149544.1"
                     /translation="MFKKILIANRGEIALRIISACRELDIRTVAIYSEADRDALHVRY
                     ADEDVCVGPYPSAASYLNISAIVSAAEITEADAIHPGYGFLSENAYFAEILQDCHISF
                     IGPSPDAMRKMGDKSLARRTVAAAGVPTVPGSPGPLESVDDALAFARKAGYPVILKAA
                     AGGGGRGMRVARDERELSGAYETARAEADKAFGNSDVYLEKYLEAPRHVEFQIFGDSH
                     GNVQHMGERECSIQRRHQKLIEEAPSTALTPELRRQMGAAAVTAAKAVNYTNAGTIEF
                     LLDSQDRFYFMEMNTRLQVEHPVTEEVTGVDMVKEQIAVAAGEPLSFAGRQLVPRGHA
                     IEFRVNAEDPVTFAPSPGRLTAFHPPGGMGVRVDTAAYQGYVIPPHYDSLIAKLVVRG
                     RDRPEAIARARRALDLFVIEGIKTSIPMHRRILDDPDFLVGRLSTRFMERFAAAS"
     gene            2865..4016
                     /locus_tag="VF958_10335"
     CDS             2865..4016
                     /locus_tag="VF958_10335"
                     /inference="COORDINATES: protein motif:HMM:NF014622.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HEX9149545.1"
                     /translation="MSAKLPPIYAITSGEARSAVEVERTAGRLFEVGIRLLQVRERLL
                     TDCELLEAVEAAAGLAPEFGATVIVNDRADVARLAGVGVHLGEEDLPAAFARPLLAPG
                     APLGVSTHDLAAARRAFTDLSCDYVAFGPIFESPTKTGRSPRGTEALAAVAAEKTKPL
                     VAVGGITADRLDAVYDAGADSAAVVGALFSRGTLEENARVLLDRARRRTPPGRLYLVG
                     FMGCGKTAIGRRIAERTGVPFVDLDTEIERTSGLTVRAMFEAHGEPAFRERESAFLAG
                     TEALPNAVVSTGGGSYVPESNRRTIGRLGTAIFLDVPFDSILARIVGKTDRPLFVSTV
                     QAASLYGERESFYRMAPVHVTLTGRESIEESADKVLSAVYDRRELGVSL"
BASE COUNT          634 a         1409 c         1381 g          644 t
ORIGIN      
        1 gacaatcact gaaccggtgt ccccgccctc tccgccattc cctccgatcc cgacagtgcc
       61 ggacaccgtt ctcacgccgc cggcggacct cctccgacgg cctgcgtcga tcgaagagcc
      121 ccctgcgccg cggcgcgaca ccgatccctt cgacatcagc gacatcccct acaagccccc
      181 cggcgttcca gccgtgccga tctccgacag accggacgtg ttctcgcgcg acgtttcgct
      241 cgactcgata atttcgccga agagggaatc cgacggggag atcatcacga agaagattcg
      301 gcttcccgag gcgatgtggc ccttcgagcc gcccgccccc gccggaccgg ccgaggcgcc
      361 gtccccgccg ccgtcgcctc catcggagcc ggccccgccg gagccaacgg ggcgcacgct
      421 cgcggatctc tacttcgacc agggtcatta cccggaagcg ctggcgatct accgggagct
      481 ccttgccgcg gatccggcca acgaggacct cgcacggttg cgggccgacg ccgagcgtct
      541 cgcggccgcg acgcctcccc ggcagctccc gccgccggac cccggccggc agcggcgact
      601 cgagaagatc cgggttctga acgaatggct cgacgtcata cggacgtcgg ccgcggccga
      661 tcgtgatggg cgcattcgct gagagcctcg cgcggattgc ccgccgcgtt gagggggcgc
      721 gggcggtctc attgatcggc gccgacggga tcccggtcga aacctgggga tccgtagaag
      781 gcctttcctt cgaggtactt gcggcggaga tggccggcct gctgaaatcg gcccggagcg
      841 cgggcggagc ggccggcgcc ggacccctcc gcgaagtcgc gaccgtcacc gacggggcga
      901 tcacgatcct ttcccgcgtg accgcggagt attatctgct gctactgttg ggtcgcgagg
      961 gcaacctcgg gcgcggccgg ttcgagctgc gcaaggccgc ggcggatctc gaaaaggagc
     1021 tggtttgagc cgatgctctc tttcaaagaa atcaaagaat tgatcgacct cgtctcggaa
     1081 cggggcctcg ccggcgtcga gatcgagcgc gccggttttc gcctccgcat cgaaggcgcc
     1141 cggccggcgg cagtcaccgc gccggccgag gcggtcccgg ctccatcgcc ggcggctgcg
     1201 gcgcccgcgg ccgcggctcc cacggcggcg gcggccgagg ccgcgcgaga gacggagggc
     1261 atccacgtca tcacctcgcc catcgtcggg accttctacc gggctccctc tcccgaagcg
     1321 gacgcgttcg ccgaggtagg cgagcgggtc gcccgcggca aggtcctctg catcatcgag
     1381 tcgatgaaac tgatgaacga aatcgagtcg gacgtcgagg gcgagatcgt ggccgtctat
     1441 gcccgcaacg ggcagccggt cgagtacggg gagaagcttt tcggcatccg actggcggct
     1501 tagcccggtc cacctgtgtt caagaaaatt ctgatcgcca accggggcga gatcgcgctc
     1561 cggatcatct cggcatgtcg cgagctcgac atccggaccg ttgccattta cagcgaggcc
     1621 gatcgcgacg ccctgcacgt gcgctacgcc gacgaagacg tgtgcgtggg cccgtaccct
     1681 tccgcggcgt cctacctgaa catctcggcg atcgtgtcgg cggcggagat caccgaggcg
     1741 gacgcgatcc atccgggata cggttttctc tccgagaacg cctacttcgc cgagatcctg
     1801 caggactgtc acatctcttt catcggcccc tctcccgacg ccatgcgaaa gatgggggac
     1861 aagtcgctcg cccgccggac ggtggcggcc gcgggggttc cgacggtgcc gggctcgccg
     1921 gggccgctcg agtcggtgga tgacgccctg gcttttgccc ggaaagcggg gtatccggtc
     1981 atcctcaagg ccgcggcggg cgggggcggg cgcgggatgc gggtggcacg cgatgagcgc
     2041 gaactgtcgg gcgcctacga gaccgcgcgg gccgaagccg acaaggcctt cggtaattcc
     2101 gacgtgtacc tcgagaagta cctcgaggcg ccgcgtcacg tcgaattcca gatcttcggc
     2161 gactcacacg ggaacgtcca gcacatgggc gagcgggagt gctcgatcca gcgccgtcat
     2221 cagaagctga tcgaggaggc gccctcgacg gcgttgacac ccgaactgcg gcgtcagatg
     2281 ggcgcggcag cggtgacggc cgcgaaggcg gtgaactaca cgaacgccgg gacgatcgaa
     2341 ttcctgctcg attcgcagga ccgcttctac ttcatggaga tgaacacgcg gctccaggtc
     2401 gagcacccgg tcaccgaaga ggtcaccggg gtcgacatgg tcaaggagca gatcgccgtc
     2461 gcggcggggg agccgctctc cttcgcgggg cggcaactcg tgccgcgcgg tcacgcgatc
     2521 gagttccggg tgaacgccga agatccggtg acgttcgcgc cgtctcccgg ccggctgacc
     2581 gcgtttcacc cacccggcgg tatgggcgtc cgcgtcgaca cggccgcgta ccagggatac
     2641 gtcattccgc cgcactacga ctcgctgatc gcgaagctgg tcgtgcgcgg ccgcgaccgt
     2701 ccggaggcga tcgcgcgcgc gcgccgggcg ctcgatctgt tcgtcatcga gggcatcaag
     2761 acgtcgatcc cgatgcaccg caggatcctc gacgaccccg acttcctcgt cgggcgactc
     2821 tcgacgcgct tcatggagcg gttcgcggcc gcgtcgtagc cgggttgagc gcgaagcttc
     2881 ctccgatcta cgccatcacc tccggagaag cccgttcggc ggtcgaggtc gagcgaaccg
     2941 ccggccgcct cttcgaagtg ggaatccgcc tcctgcaggt ccgcgagcgg ctcctgacgg
     3001 actgcgagct cctcgaagcg gtggaggcgg ccgccggcct ggcccccgaa ttcggagcga
     3061 cggtcatcgt gaacgaccgc gccgacgtcg cccgcctggc gggcgtgggg gttcacctcg
     3121 gcgaggagga tctcccggcg gcattcgcgc gtccgctcct cgccccggga gcgccgctgg
     3181 gagtttcgac gcacgacctg gcggcggcgc gccgtgcatt cacggacctc tcctgcgact
     3241 acgtcgcttt cggcccgatc ttcgaaagcc cgacgaagac cggccggtcg ccccgcggga
     3301 ccgaggcgct cgccgccgtc gccgcggaaa agacgaaacc cctcgtggcc gtggggggca
     3361 tcactgccga ccgcctcgac gccgtctacg acgccggcgc cgactcggcg gccgtcgtcg
     3421 gagcgctttt ctcccgcggg acgctcgagg agaacgcccg cgttctgctc gaccgcgcgc
     3481 gacgccgaac tcctcccggc cgcctgtacc tcgtcggatt catgggatgc gggaagacgg
     3541 cgatcgggcg gcgcatcgcc gagcgcacgg gcgtcccgtt cgtggacctg gacacggaga
     3601 tcgagcggac gtcgggactc acggttcgcg cgatgttcga ggctcacgga gagccggctt
     3661 ttcgcgagcg ggaatcggcg ttcctcgcgg ggacagaggc gctcccgaac gccgtcgtct
     3721 cgacgggcgg aggttcgtac gtgccggagt caaaccgtcg cacgatcggg cggctcggga
     3781 cggcgatatt cctggacgtt cccttcgact ccattctcgc gcggatcgtc gggaagaccg
     3841 atcgcccgct cttcgtgagc acggtgcagg cggcgtcgct ctacggcgaa cgggagtcgt
     3901 tctatagaat ggccccggtt cacgtcactc tcaccgggcg cgagtcgatc gaagagtccg
     3961 ctgacaaagt gctctcggcg gtctatgacc ggcgggagct gggggtctcg ctttagttcg
     4021 aagtccctgc cagaaatgcg atatctcatt ctgtccgaca tccactcg
//
LOCUS       DASWAV010000496         2815 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U2665
            k141_307775, whole genome shotgun sequence.
ACCESSION   DASWAV010000496 DASWAV010000000
VERSION     DASWAV010000496.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960316
            Sequence Read Archive: SRR10260007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2815)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2815)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:47:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,929
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2815
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_307775"
                     /isolate="SMAG_U2665"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.36444 N 132.31889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            99..1322
                     /gene="ftsA"
                     /locus_tag="VF958_10340"
     CDS             99..1322
                     /gene="ftsA"
                     /locus_tag="VF958_10340"
                     /inference="COORDINATES: protein motif:HMM:TIGR01174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HEX9149546.1"
                     /translation="MAKPENFLVSCDIGSSKICVLIGEQNAHSALDVIGKGAAPNRGT
                     RKGNIINVDATIEAIKRAAEEAEIMAGVQIARAWVGISGSNVRSFNSRGVVAVAGKER
                     EITREDVTRVIDAARGVQIPQDHEVVHVVPREFAVDGQDGVGDPIGMVGSRLEADVHV
                     VTAPVAVTQNIVTCMNKAGIEVVELVLEQFAAAEAVLTEDEKELGIVLIDIGGGTTEV
                     AVYQRGSIAHTAVIPIGGDHFTNDLAVVLRSPIGDAERIKKKYGSALRSAVGDDEMVE
                     VPMVGGREPKLCPRATLADILQPRAEELLGLIQEDLQRLGVDKEIRSGVVLTGGGAEL
                     EGIVEVAEGIFEGPVRRGVPVGVGGLVDVVSRPEWATATGLLLYGHRNKTGRRRTRGF
                     TRAAGSVSRFFRELF"
     gene            1388..2548
                     /gene="ftsZ"
                     /locus_tag="VF958_10345"
     CDS             1388..2548
                     /gene="ftsZ"
                     /locus_tag="VF958_10345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020374582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HEX9149547.1"
                     /translation="MIELVDDGRSRLAVELERGDASPAVIKVIGIGGGGCNAVNRMIA
                     AGVRGVDYIACNTDLQALRKSGATTKLQLGARLTKGLGAGADPDVGRNAAIEDQEKLS
                     QIVQGADMVFLAAGLGGGTGSGAGPVIAKLAAESGALTVAVVTKPFDFEGKKRMRQAE
                     KAARELREHVDTLITIPNQRLLAFVERNTPFNESFKIADDVLRQAIQGIADLINCPGE
                     INRDFADVRKIMSDMGRAMMGTAIAEGENRAVHAAQAAISSPLLEDASIEGARGVLIN
                     VTGSADLTLFEVNEAAEIIRQAADPEAVILFGYVNDDRMAGSVKVTVIATGFDRADAA
                     EEERQQEEESRHAPPRAGRAFLRGDDTGGFGPNASSSRDDFDIPTVLRRQMD"
BASE COUNT          506 a          857 c         1007 g          445 t
ORIGIN      
        1 cctatgagcc ggaaatcgga aatcggtaat cgcgaatcga agatcgcctg cactcggtcc
       61 gattaccgac ctccgattac cgcttgccgg gttttctgat ggccaagcct gagaacttcc
      121 tcgtctcctg cgacatcggg agctcgaaga tctgcgtgct gatcggcgag cagaacgccc
      181 attccgcgct cgatgtgatc gggaaggggg ccgcccccaa ccgcgggacg cgcaagggca
      241 acatcatcaa cgtcgacgcc acgatcgagg cgatcaagcg cgctgcggaa gaagcggaga
      301 tcatggcggg cgttcagatc gcccgtgctt gggtggggat ttccggctcg aacgtgcgct
      361 ccttcaacag ccgcggcgtc gtcgccgtgg ccggcaagga gcgggagatc acgcgtgaag
      421 acgtcacgcg ggtcatcgac gccgcccggg gcgtgcagat cccgcaggac cacgaggtcg
      481 tccacgtcgt tccccgagag ttcgccgtgg acggacagga cggcgtcggc gaccccatcg
      541 ggatggtggg ctctcggctg gaggcggacg tgcacgtggt gacggcgcct gtcgccgtaa
      601 cccagaacat cgtcacctgc atgaacaagg caggaattga ggtcgtcgag ctggtcctcg
      661 agcagttcgc ggcggcggaa gccgtcttga ccgaagacga gaaggagctc ggcatcgtgc
      721 tgatcgacat cgggggcggg accaccgagg tcgccgttta ccagcggggg tcgatcgcgc
      781 acacggccgt gattcccatc ggaggcgacc acttcaccaa cgacctcgcc gtcgtgctgc
      841 gctcgccgat cggcgacgcc gagcggatca agaagaagta cggctccgct ctccgctcgg
      901 cggtcgggga cgacgagatg gtcgaggtgc cgatggtcgg cggccgggag ccgaagcttt
      961 gcccccgcgc caccctggcc gacatccttc agccccgcgc agaagagctt ctcggtctga
     1021 tccaggagga cctgcagagg ctcggcgtcg acaaggagat ccgatcgggc gtcgtcctga
     1081 cgggaggggg cgcggagctc gaaggaatcg tcgaggtggc ggagggaatc ttcgaagggc
     1141 cggtccgccg gggcgtaccc gtcggggtcg gaggtctcgt cgacgtcgtg tcccgtccgg
     1201 agtgggcgac ggcgacggga ctcctgctct acgggcatcg caacaagacc gggcggcggc
     1261 ggacgcgcgg gttcacgcgc gccgcgggtt cggtctcccg gttcttccgg gagctgttct
     1321 gagggatgga cgggcgggtg agcgccgcga gaggcgaagt ccaggaaagg gtagaaggga
     1381 gaggtccatg atcgagctcg tcgacgacgg aagatcgcgc ctcgcggtcg aactggaacg
     1441 gggagacgca tctccggcgg tcatcaaagt catcgggatc ggcggcgggg gctgcaacgc
     1501 cgtcaaccgg atgatcgcgg cgggcgtgcg aggggtcgac tacatcgcgt gcaacacgga
     1561 cctccaggcg cttcggaagt ccggggccac gacgaaactt cagctcggtg cgcgattgac
     1621 gaaagggctc ggcgccggcg cggatcccga cgtgggtcgc aacgccgcga tcgaggacca
     1681 ggagaagctc tcgcagatcg tgcagggcgc ggacatggtg ttcctcgccg cggggctcgg
     1741 aggcggaacg ggatcgggcg cggggccggt catcgcgaaa ctcgcggcgg agtcgggagc
     1801 gctcacggtc gccgtggtaa ccaagccgtt cgacttcgag gggaagaagc ggatgcggca
     1861 ggcggagaag gcggcgcgcg agctgcgcga gcacgtcgac acgctgatca cgattcccaa
     1921 ccagagactg ctcgccttcg tcgagcgcaa cacgcctttc aacgaatcgt tcaagatcgc
     1981 ggacgacgtc ctgcggcagg cgatccaggg gatcgcggac ctgatcaact gtccgggcga
     2041 gatcaaccgc gacttcgcgg acgtccggaa gatcatgtcg gacatgggcc gcgcgatgat
     2101 gggcaccgcg atcgccgagg gcgagaaccg ggcggtccat gcggcacagg cggcgatctc
     2161 gtcaccgttg ctcgaggacg cgtcgatcga aggcgctcgc ggagtcctga tcaacgtcac
     2221 cggcagcgcg gacttgacgc tcttcgaggt caacgaggcg gcggagatca ttcggcaggc
     2281 cgcggacccc gaagcggtga tcctcttcgg ctacgtcaac gacgaccgca tggcgggatc
     2341 ggtgaaggtc acggtcattg ccacaggctt cgaccgggcc gacgcggccg aggaggagcg
     2401 gcaacaggag gaggagtcgc gtcacgcccc gccgcgtgcg ggacgggcgt ttctccgcgg
     2461 cgacgacacc ggcggtttcg gacccaacgc gtcttcgtcg cgtgacgact tcgacatccc
     2521 gacggtgctg cgccgccaga tggattagcg ggtcagatct ccagttgcca cttgcttctc
     2581 aaggcggcag accactcatt cggatggagc cagtttgtga cccgtgaaga cctgacccta
     2641 ctgatgcatt ggcatcagtc cctccgattg cggcggcgct agcttcgagc cgtactcgga
     2701 aaggggggag aagatgacgc actctcccgg gttcctgatt cttgccgtcg tcgcggcagg
     2761 gttgttcttc gttttgcttc cagtgatgct cgcgaccttc tccgaatatc gtcga
//
LOCUS       DASWAV010000497         5259 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U2665
            k141_748313, whole genome shotgun sequence.
ACCESSION   DASWAV010000497 DASWAV010000000
VERSION     DASWAV010000497.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960316
            Sequence Read Archive: SRR10260007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 5259)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5259)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:47:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,929
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5259
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_748313"
                     /isolate="SMAG_U2665"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.36444 N 132.31889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..819
                     /locus_tag="VF958_10350"
     CDS             <1..819
                     /locus_tag="VF958_10350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9149548.1"
                     /translation="TVLNPLPFGTTPASLNLLLAGPTTDYALFPVLRENASGASFDGT
                     VATYTFRNAIPADASGTWAASIEVRRTVTLNPGAQGQQTVREAAFNPVKYFAVTDSEP
                     VGRRIVIDVNMTKCNECHDRLALHGTQRQNAQECVFCHNPNGNDGRDVPESIDFKRMI
                     HRIHTGEELTQDYSIGNGSFNEVRFPGDRRDCVACHASTSTYQLPLPEGLLPTATPRD
                     YYSPQQPTATACLGCHDTRSAAAHAFVNTAPFGEACASCHGPNAEFAVDKVHAR"
     gene            872..1804
                     /locus_tag="VF958_10355"
     CDS             872..1804
                     /locus_tag="VF958_10355"
                     /inference="COORDINATES: protein motif:HMM:TIGR03508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DmsE family decaheme c-type cytochrome"
                     /protein_id="HEX9149549.1"
                     /translation="MSALLIAFAVAALTAATARAQSTAPEATPTPAAAVAPAAGAAAL
                     CGDCHEDQMKAFAANPHARMPEKWRPTAGSPNSLCESCHGDGTKHIEGGGDKALIRGF
                     HTGGDADTCLNCHVPRGDHSGLRGGIHTNSATVRCTTCHSVHNPTPKSPHLLAKAPVQ
                     LCATCHVVQTASLATKPFAHRLGRGGLDCTSCHDPHGRPGKESLKRTRDDELPCLNCH
                     AEKRGPFVFEHPPKGVVGDCTTCHQPHGSMNPKQLTRANVYQLCLECHSTLSAATLGS
                     QPPSFHNISLPRYRNCTTCHVAIHGSNLSPTFFK"
     gene            1812..3758
                     /locus_tag="VF958_10360"
     CDS             1812..3758
                     /locus_tag="VF958_10360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9149550.1"
                     /translation="MRPERIAAFLLGATLFLAPAGASGQAVPLDLELGYRFVDINGNR
                     DMYRSQINDREGILLRSLTFSTGTIGGSFIDSLRVDAFDLGAGPAGRFRLEAGKTGLY
                     RLRLDYRRMEFFSALPAFANPLLTSGIIPGQHTYDRTRHLLDVEMEILPNGVLTPIVG
                     YTLNRYDGPARTTYDVGGDEFRLASDLEDTEQEARVGVAFHAGDFSGRVVQGWRQFKE
                     TEHLTLVPGAGAGNNAGPVLGTPVSLTDFQRDSRVKVNVPVTTAYVVGRFASRVKVSA
                     GYVRANSDTSASETEDLTGNLVSFQISRFFHGLSEPISSRARNEQWSGHGRVEFEIAD
                     GLDLSAGYRQRHRELNGFALISSLFLDTLTFSGQDPRNLERIIEANTSLERTEKIFDA
                     SFGARLLGPVALGVGYEQNDQDVRITPDPEEIVVPGGQGGNFDRKIRSWNASANFTQS
                     GVTLGADYRHDKADDAIVRVDFIKRDRYRVRAAWSLADHIRLSANGEQVDVSNDRPGI
                     GYDGRIRQYGGDLEVAPVKPFTLRFSASRFDARSTIPIRQPQDFSVITSRNQEKGTWL
                     EGGAALNLSRFRLEGAVGRLKNEGTFPFNIDRARAQLEFDWNARFTSVLDWNKDKYSE
                     SPQHNGNLGGFDANRYGIFLRVHL"
     gene            3803..4087
                     /locus_tag="VF958_10365"
     CDS             3803..4087
                     /locus_tag="VF958_10365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9149551.1"
                     /translation="MKRKGSNVGLIAMALFWAGWMAFSSVSHATLPIQKKAKELGYPA
                     TNCQYCHVEKLPKKGAMTHNERGKWLEAEKVKRGAKEVDAAWLKDYPGGK"
     gene            4170..>5259
                     /locus_tag="VF958_10370"
     CDS             4170..>5259
                     /locus_tag="VF958_10370"
                     /inference="COORDINATES: protein motif:HMM:NF025890.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c3 family protein"
                     /protein_id="HEX9149552.1"
                     /translation="MKLASAAILLCLAAARLTAEPVKSSPAPTNEDCLTCHQDPAAKR
                     ANGTSISVSKDKLAASAHGQAGLACVDCHGDLAKTQNFPHKEKLKKVDCAACHDSVGA
                     AHPFHPEIARAAEGKGKAQVDCADCHGSHEIAPVKDPGFPFTAERQPQACGSCHDEIR
                     DHFVSSEHGKALASGRKPAPSCLSCHRQGVTAGSGLEQSALKKKQEDLCLSCHLYNES
                     VKARAAPAGNFIASFQHSVHGMALQRGNLRAPNCVTCHGAHEERHGFDTSSLVNKMRV
                     QDVCGKCHSKENREFSASVHGAAIERGKRDAPACTDCHGEHNILSPKDLRSPVAAANI
                     SARVCTPCHGSLELTEKYNLPANRSRTFA"
BASE COUNT          971 a         1791 c         1671 g          826 t
ORIGIN      
        1 accgtcctga acccgctgcc attcggcacc accccggcca gcctgaacct gctgctcgcc
       61 gggccgacga cggactacgc gctgtttccc gtgctccgcg agaacgcgag cggcgcttcc
      121 ttcgacggca cggtcgcgac ctataccttc cgaaacgcga tcccggcgga cgccagcggc
      181 acctgggcgg cctcgatcga ggtgcggcgg accgtcacgt tgaacccggg agcccagggc
      241 cagcagaccg tccgggaggc ggctttcaac ccggtgaagt acttcgcggt gaccgactcg
      301 gagcccgtcg ggcggcgaat cgtcatcgac gtcaacatga cgaagtgcaa cgagtgtcac
      361 gaccgtctgg cgctccacgg aacgcagcga cagaacgccc aggagtgcgt cttctgccac
      421 aacccgaacg ggaacgacgg acgtgacgta cctgagtcca tcgacttcaa gcggatgatc
      481 caccgcattc acaccggaga ggaactcacg caggactact cgatcgggaa cggcagcttc
      541 aatgaagtcc gtttcccggg agaccggcgc gattgcgtcg cctgtcacgc cagcaccagt
      601 acgtaccagt tgccgctccc cgagggtctg ctgccgacag cgacgcctcg cgattactat
      661 tcgccccagc aaccgaccgc gacggcgtgc ctcgggtgcc acgacacgcg gtccgcagca
      721 gcgcacgcct tcgtcaacac ggcgcccttc ggagaggcct gcgcatcctg tcacggcccg
      781 aacgccgagt tcgccgttga caaggtccat gcgcgctagc ggggggaggg accgagatct
      841 ccgcgcccgc ccgtttcgtc gggcgcggag tttgtccgct ctcctgattg cgttcgcggt
      901 tgccgcgctg acggcggcaa ccgcccgggc acagagcacg gcccccgagg cgacgcccac
      961 tccggccgct gccgtcgcgc cggctgccgg agccgcggct ctctgcggcg actgtcacga
     1021 agaccagatg aaggccttcg ccgccaatcc gcacgcgcgg atgcccgaaa agtggcgccc
     1081 gaccgcgggt tctcccaact cgctgtgcga gagctgccac ggcgacggca ccaagcacat
     1141 cgaagggggc ggggacaagg cgctcatccg cggatttcac acgggcggcg acgccgacac
     1201 gtgcctgaac tgtcacgtcc cgcggggcga ccactccggg ctccgcggcg ggatccacac
     1261 gaactccgcg accgtccgct gcacgacctg ccactcggtt cacaacccga cccccaagag
     1321 cccgcacctc ctggcgaagg ccccggtgca gctctgcgcc acatgtcacg tcgtgcagac
     1381 cgcctcgctc gcgaccaaac ccttcgcgca ccggctcggc cggggaggcc tcgactgcac
     1441 gtcgtgccac gacccgcacg gccggcccgg caaggagagc ctgaagagaa cgcgcgacga
     1501 cgagctgccg tgcctgaact gtcacgccga gaagcgcggg ccgttcgtgt tcgaacatcc
     1561 gccgaaggga gtggtcgggg actgcaccac ctgtcaccag cctcatggat ccatgaatcc
     1621 gaagcagttg acgcgcgcga acgtctacca gctgtgtctc gagtgccact cgacgctctc
     1681 cgcggcaacg ctcgggtcgc agcccccctc cttccacaac atctctctgc cgcggtaccg
     1741 caactgcacg acctgtcacg tcgccattca cggctccaac ctttccccca cgttcttcaa
     1801 gtgagggtgt gatgagaccc gaacggattg cggctttcct cctcggggcc accctgtttc
     1861 tcgcaccggc gggggcgtcg ggccaggcgg tcccgctcga tctcgagctc gggtatcgct
     1921 tcgtcgacat caacggcaac cgcgacatgt atcgcagcca gatcaacgac cgcgaaggga
     1981 tcctcctgcg gtcgctcacg ttctcgaccg ggacgatcgg cggctcgttc atcgactcgc
     2041 tgcgcgtgga cgccttcgac ctcggcgccg gccccgcggg gaggttccgc ctcgaagcgg
     2101 ggaagaccgg cctctatcgg ctgcgcctcg attaccggcg gatggagttc ttcagcgcgc
     2161 tgccggcgtt tgccaatccg ctgctcacga gcgggatcat ccccggccaa cacacgtacg
     2221 accggacacg tcacctgctc gacgtcgaga tggagattct tcccaacggc gtcctgaccc
     2281 cgatcgtcgg ctacaccctg aaccggtacg acgggccggc ccgcacgacc tacgacgtgg
     2341 ggggagacga gttccgcctc gcttccgacc tcgaggacac cgagcaggag gcccgggtcg
     2401 gcgttgcgtt tcatgccggc gatttctccg gtcgtgtggt ccagggctgg cggcagttca
     2461 aggagacgga gcacctgacg ctcgtccccg gggcaggcgc ggggaacaat gccgggccgg
     2521 ttctcggtac gccggtttcg ctgacggatt tccagcgcga ttcgcgcgtg aaggtgaacg
     2581 tgccggtcac caccgcctac gtggtgggcc ggttcgcgag ccgcgtgaag gtttcggccg
     2641 ggtatgtccg ggcgaactcc gatacgtccg cctcggagac ggaagacctg accggcaacc
     2701 tcgtctcgtt ccagatcagt cgtttcttcc acggactctc cgagccgatc tcctcccgcg
     2761 cgcgcaacga gcagtggagc ggccacggcc gcgtcgagtt cgagatcgcc gatggtctgg
     2821 acctctccgc cggctacagg cagcgccacc gcgagctcaa cggcttcgcg ctcatctcga
     2881 gcctcttcct cgacacgctg acgttctccg ggcaggaccc gcgaaatctc gaacggatca
     2941 tcgaagcgaa tacctcgctc gagcggaccg agaagatctt cgacgcctct ttcggcgcac
     3001 gcctcctcgg gcccgtcgct ctcggtgtgg gttacgagca gaacgaccag gacgtgcgca
     3061 tcacgcccga tccggaggag atcgtcgtgc ccggcgggca gggcggcaac ttcgaccgaa
     3121 agatccgttc gtggaacgcc tcggcgaatt tcacgcagag cggggtcacg ctcggggccg
     3181 actaccgcca cgacaaggcg gacgacgcca tcgtgcgcgt cgacttcatc aagcgtgacc
     3241 ggtaccgggt gcgcgccgcc tggtcgctcg ccgaccacat ccggctgtcg gcgaacgggg
     3301 agcaggtgga cgtctccaac gaccggcccg ggatcgggta cgacgggcgt atccgacagt
     3361 acggcggcga cctcgaagtc gcgccggtga agcccttcac cctccggttt tccgcgagcc
     3421 gcttcgacgc gaggagcacg attccaatcc gtcagccgca ggacttctcg gtcatcactt
     3481 cgcggaatca ggagaaaggg acgtggctgg aaggcggcgc cgcgctgaat ctctctcggt
     3541 tccgcctgga aggcgccgtc ggacgactca agaacgaggg gacgttcccg ttcaacatcg
     3601 accgcgcgcg ggcccaattg gaattcgact ggaacgcccg tttcacgagc gtcctcgatt
     3661 ggaacaagga caagtacagc gagtctccgc agcacaacgg caacctcgga ggcttcgacg
     3721 cgaatcgcta cggaattttc ctgagagttc atctgtaggt tggggggaat cgtaaagact
     3781 ggacgcaaaa ggggaggtac agatgaagag aaaaggatcg aacgtcggat tgatcgcgat
     3841 ggcgttgttc tgggccggct ggatggcatt cagctccgtg tcgcacgcaa cgcttccgat
     3901 ccagaagaag gcgaaggagc tcggatatcc cgcgacgaac tgccagtact gccacgtcga
     3961 gaagctcccg aagaagggcg caatgactca caacgagcgc ggaaagtggc tggaggcgga
     4021 gaaggtgaag cggggagcga aggaagtcga cgcggcctgg ctcaaggatt atccgggcgg
     4081 aaaatagtcg ggtcactctc acgggccggt cggcagcggg ctgaccggcc cttgttttgt
     4141 cggcgcaccc tcccggagag gagcggcgag tgaagctggc atcggcggcg atccttctgt
     4201 gcctggcggc agcccgcctg acggccgagc cggtgaagtc ttctcccgct ccgacgaacg
     4261 aggactgcct cacctgccac caggatccgg ccgccaaacg cgcgaacggc acctcgatct
     4321 ccgtctcgaa agataagctc gcggcgtccg cgcacggaca ggcggggctc gcgtgcgtgg
     4381 actgccacgg cgatctcgcc aagactcaga actttcccca caaagagaag ttgaagaagg
     4441 tcgactgcgc ggcgtgtcat gactccgttg gcgcggcgca tccgtttcac ccggagattg
     4501 cccgcgcggc ggaggggaag ggaaaggccc aggtcgactg cgcggactgc cacggatcgc
     4561 acgaaatcgc gccggtcaag gacccgggct tccccttcac agcggagagg cagccgcaag
     4621 cctgcggaag ctgccacgac gaaattcgcg accacttcgt ctcctccgag catgggaaag
     4681 ccctcgcttc agggcgtaag ccggcgccga gctgcctctc ctgccatcga cagggggtca
     4741 cggcgggatc cggtctcgaa cagtcggccc tgaagaagaa gcaggaggat ctctgtcttt
     4801 cctgtcacct ttacaacgaa tcggtcaagg cgcgagcggc gccggccggc aacttcattg
     4861 cttcctttca gcacagcgtt catggaatgg cgttgcagcg cggaaacttg cgagcgccca
     4921 actgcgtgac ctgccacggc gcccatgagg agcgtcacgg cttcgacacc tcttcactcg
     4981 tcaacaagat gcgcgtgcag gatgtctgcg ggaagtgcca ctcaaaggag aaccgggagt
     5041 tctccgccag cgtccacggc gcagcaatcg aaagaggcaa gagggacgct ccggcatgca
     5101 ccgactgcca cggagaacac aacatcctct ctccgaaaga cctccgttcg ccggtggccg
     5161 cggcgaacat ctccgcgcga gtctgcacgc cctgccacgg ctcgctggag ctgacggaga
     5221 aatacaacct tccggcgaat cggtcccgga cgttcgccg
//
LOCUS       DASWAV010000498         7161 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U2665
            k141_347022, whole genome shotgun sequence.
ACCESSION   DASWAV010000498 DASWAV010000000
VERSION     DASWAV010000498.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960316
            Sequence Read Archive: SRR10260007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 7161)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7161)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:47:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,929
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7161
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_347022"
                     /isolate="SMAG_U2665"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.36444 N 132.31889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(12..1631)
                     /locus_tag="VF958_10375"
     CDS             complement(12..1631)
                     /locus_tag="VF958_10375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011681837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GspE/PulE family protein"
                     /protein_id="HEX9149553.1"
                     /translation="MAKQYVNEVRVSTREEDEAAALALAERLGLPYVDIASFRIDPDL
                     FRSIPVEWMLRYTFVPEKRTDKSMTIICADPTDVVKLDELELLTGLPLKIKVGSRAAI
                     ADILQKSESSQRVLDEATEEFRLQLVSEDEEGEETLTIDRITQDASPIIKLVDSIVFN
                     AIQRRASDVHIETQENVVLIKYRIDGVLYQAMDPIDKRHHGTIISRIKVMSELDIAEK
                     RIPQDGRFKLRVRGRTIDFRVSIMPTVHGEDAVIRILDKESANAEFKSLNLDVLGFDT
                     ETKKKLRKFIREPYGMVLVTGPTGSGKTTTLYACLSEIQSVEDKIVTIEDPVEYQLRG
                     ITQIPVNEKKGLTFARGLRSILRHDPDKIMVGEIRDEDTAQIAVQSALTGHLVFTTVH
                     ANNVVDVLGRFLNMKVELYNFVSALNCVLAQRLVRKICIHCREKVEVSPQAILEAGLD
                     PASVAGRVFYEGRGCLECDGTGFHGRVAIAELLDLSDRIRGLILERRPAAEIKKAAKE
                     EGMTFLREAALAKVFEGSTTLKEINKVTFVD"
     gene            complement(1747..2961)
                     /locus_tag="VF958_10380"
     CDS             complement(1747..2961)
                     /locus_tag="VF958_10380"
                     /inference="COORDINATES: protein motif:HMM:NF012694.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HEX9149554.1"
                     /translation="MSPQFSVKAGWPDGSVAEQSILAPDSKTAQAEIERRGGHVFEVR
                     RHGIALRGGRGDRKGRVKMADFLVFNQEMIALLKAGLPVVRSFEILLERQESPALRAV
                     LADVKDRVNAGASISDAFAEQGDIFPRLYWTSLKAGEKSGEIEAVLRRYLKYQKTVIA
                     ISRKVVSTLVYPAILMVMSAALIAVLMTVVIPKFQDFFADFHADLPLLTVVVVGIAGF
                     LRDNILWLLPAALVGGWVAWRWLRTPRGREWSDTMLLKVPFLGGLFRRFAITQFTRSL
                     ATLLGGGTPLVPSLENASEAIGNRFISRRVRGVVPKVREGGELWQALDATGIFTNLTI
                     EMIKVGETSGALEEMLSAVSDFYDEEIDVLLARVITLVEPAILVMMGGIIITILMSVY
                     LPIFKIMSQIKG"
     gene            complement(2958..3707)
                     /locus_tag="VF958_10385"
     CDS             complement(2958..3707)
                     /locus_tag="VF958_10385"
                     /inference="COORDINATES: protein motif:HMM:NF015891.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="secretin N-terminal domain-containing protein"
                     /protein_id="HEX9149555.1"
                     /translation="MRKKIFAAAIGAFSLFAPAIFGAEEAAVKVFILKYRQVEEAALL
                     IRPHLSDAGTVTLTPRLRAITVTDREENLRTIARVISQFDAPPRSFTFAIKLVRARAE
                     VPAGSIDREIGGMGAKLKSLFQFNDYSLIDSAVMRGIEGGPVAAQLGEEGDAYMLNFW
                     IEPAGAAEQQLMLSPFSLSRLRRNAQGRVVPVALYRAPMAVTLNQTLVVGASKEEGSK
                     NALILILLAQETPRVGAEKGTEGQRSVEKKP"
     gene            complement(3794..4357)
                     /locus_tag="VF958_10390"
     CDS             complement(3794..4357)
                     /locus_tag="VF958_10390"
                     /inference="COORDINATES: protein motif:HMM:NF024881.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma factor"
                     /protein_id="HEX9149556.1"
                     /translation="MSASCGTVRERISPYLADALTADDRREFREHLASCPACRDRVAG
                     VEPSLLFSRVGPEEVSAEDVARVLAGVRTGIALVRTESRVGRARRGSRRRVAAMASAA
                     ALAAMTLVLPGSGRRPDDTVSAPLTASASAPAPGADLAPAKGFSPASERAPSATFPAD
                     ATIYDWNPGAASQEPRVVWIVDRSLDI"
     gene            complement(4354..5028)
                     /locus_tag="VF958_10395"
     CDS             complement(4354..5028)
                     /locus_tag="VF958_10395"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HEX9149557.1"
                     /translation="MLRGQALRACTRRRDMTMELVYSESRDRREAAELSDTELAVRAR
                     GGDMVSFEALVVRKTPAVVSVARRVVGDGEDARDVAQLVFLRVWEQISRYDEKYSFNT
                     WLYRIATNLSIDFLRSSRSRERAHGATLHLVRLREEMTGSEAMNAVEDGELARLFQVV
                     AARLTGKQKAVFVLKEMEDRDTKEIASILNCGESTVRNHLFNARRILRREIAKICPGL
                     LDGRRS"
     gene            5221..6510
                     /locus_tag="VF958_10400"
     CDS             5221..6510
                     /locus_tag="VF958_10400"
                     /inference="COORDINATES: protein motif:HMM:NF016311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase N-terminal domain-containing
                     protein"
                     /protein_id="HEX9149558.1"
                     /translation="MQHNRRGMLFYRILATGALLAYSPVALVKAVTGRRRPGDVRGRL
                     GLSPYPDLAGGIWIHAVSVGEVGVAVPLLAALKRLAPDRRFGLSVTTAAGRELARRVA
                     PSGVDVFAFPFDLAAPVERALARVRPGLVLLTETELWPLFLTRAAERGIPVALVNGRI
                     SQRSFPRYRMLRRWFAPALDRVALFVMQSEEDARRIEALGVAASKIRMRGNIKYDRPA
                     APPFRDAERLIAAAAGRPLFVAASTDEAEEATIVAAARALAPHALTAIAPRRPERFDE
                     AARKIEGAGLSVLRRSGPERVPADVYLLDTIGELASLYSHAKIAFIGGSLVARGGHNP
                     IEAWAAGVPVIVGPHTENFREVTAKGEELAIATRISRGEELVDCVERELADRHALEAR
                     GAAAREFVSANRGAAEATAVEVLPLLTGSPLSSGAAR"
     gene            6507..>7161
                     /gene="lpxK"
                     /locus_tag="VF958_10405"
     CDS             6507..>7161
                     /gene="lpxK"
                     /locus_tag="VF958_10405"
                     /EC_number="2.7.1.130"
                     /inference="COORDINATES: protein motif:HMM:NF014645.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009029 - tetraacyldisaccharide 4'-kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetraacyldisaccharide 4'-kinase"
                     /protein_id="HEX9149559.1"
                     /translation="MRPTRLPGPVVRAALRPFSSAYGRALETRARLYRSGALASERVS
                     APVISVGNLTFGGTGKTPFVELLARRFLFEGRRPAVVSRGYGRGSKGVVVVSEGQGPR
                     VGPQEGGDEPVAIARRVPGVVVVVGEKRADAARAAIDHGADLILLDDGFQHLALRRDV
                     DLLLLDAADPFGGGRLPPDGRLREPLSALSRADGVVFTRIDRGEPPAGVVAELARRNP
                     "
BASE COUNT         1136 a         2463 c         2352 g         1210 t
ORIGIN      
        1 cggaattcgc atcagtcgac gaacgtgacc ttgttgattt ccttcagcgt cgtcgagccc
       61 tcgaacacct tcgcgagcgc cgcctccctg aggaaggtca tgccctcctc cttcgccgcc
      121 ttcttgattt ccgcggcggg acgccgctcg aggatcagcc ctcggatccg gtcggagagg
      181 tcgaggagct ccgcgatcgc cacgcgtccg tggaagccgg tgccgtcgca ctcgagacac
      241 ccccgcccct cgtagaaaac ccggccggcg accgacgccg gatcgagccc cgcttcgagg
      301 atcgcctggg gcgacacctc gaccttctcg cggcagtgga tgcagatctt ccggacgaga
      361 cgctgcgcca gcacgcagtt cagagcggac acgaagttgt agagctcgac cttcatgttg
      421 agaaagcgcc cgaggacgtc gacgacgttg ttcgcgtgga cggtcgtgaa gacgaggtgg
      481 ccggtgagcg ccgactgcac ggcaatctgt gccgtgtcct cgtcgcggat ttcgccgacc
      541 atgatcttgt cggggtcgtg ccgcaggatc gaccggaggc cgcgcgcgaa cgtgagtccc
      601 ttcttttcgt tcaccggaat ctgggtgatg ccccggagct ggtactcgac gggatcctcg
      661 atcgtgacga tcttgtcctc gaccgattgg atctcagaca ggcacgcata gagggtcgtc
      721 gtctttcccg agccggtcgg gccggtgacg agcaccatcc cgtacggctc gcggatgaac
      781 ttgcgcagtt tcttcttggt ttctgtgtcg aatccgagga cgtccaggtt caaggacttg
      841 aactcggcgt tcgcggattc cttgtcgagg attcgtatga ccgcgtcctc gccgtggacc
      901 gtcggcatga tcgaaacgcg aaagtcgatc gtccgcccgc ggactcgcag cttgaaacgg
      961 ccatcctgag ggatgcgctt ctcggcgatg tcgagctccg acatgacctt gatcctcgag
     1021 atgatcgtgc cgtggtgacg tttgtcgatc ggatccatcg cctggtagag cacgccgtcg
     1081 atccggtact tgatcagcac gacgttctcc tgcgtctcga tgtggacgtc ggaggcacgg
     1141 cgctggatcg cattgaacac gatcgagtcg acgagcttga tgatcggaga cgcgtcctgc
     1201 gtgatccggt cgatcgtgag ggtctcttca ccctcttcgt cctcgctgac gagctgcagg
     1261 cggaactcct ccgtcgcctc gtcgaggacg cgctgagagg actcgctctt ctggagaatg
     1321 tcggcgatcg cagctcggct gccgactttg atcttgagcg ggagccccgt cagcagctcg
     1381 agctcgtcca gcttcacgac gtccgtcgga tccgcgcaga tgatcgtcat ggacttgtcg
     1441 gtgcgcttct ccggcacgaa ggtgtaccgc agcatccact cgaccggaat cgagcggaag
     1501 aggtcgggat cgatccggaa cgacgcgatg tcgacgtagg gcaggccgag tcgctccgcg
     1561 agcgcgagcg cggccgcctc gtcttcttcg cgggtggaga cccggacttc gttgacgtac
     1621 tgcttcgcca tagctagacc cgccgcggcc gctgcgcaac cggccagacg agactttcag
     1681 accggagctt ccgtcgcgtc gtcatttccg ccctcccggg gaagagactc catccgcttg
     1741 caaggttcat cccttgatct gggacatgat tttgaagatc ggcaggtaga cagacatcaa
     1801 gatagtgatg atgatgcctc ccatcatcac gaggatcgcc ggctcgacga gcgtgatcac
     1861 gcgcgcgagc aggacgtcga tctcttcgtc gtagaagtct gagaccgcag acaacatttc
     1921 ctcgagcgcg ccggacgtct cgccgacctt gatcatctcg atcgtgaggt tcgtgaagat
     1981 gcccgtggcg tcgagcgcct gccacagctc ccctccctcg cggaccttcg gcaccacccc
     2041 ccggacacgc cgcgagatga agcggtttcc gatcgcctcc gatgcgttct cgaggctcgg
     2101 aacgaggggc gtgccgcccc cgagcagggt ggcgagactc ctcgtgaact gcgtgatcgc
     2161 gaaccgccgg aagagaccgc cgagaaacgg aaccttcaag agcatcgtgt cggaccattc
     2221 ccgtccgcgc ggagtgcgca gccagcgcca ggcaacccaa ccgccgacga gcgccgccgg
     2281 cagcagccac aggatgttgt cgcgcaggaa tcccgcgatt ccgacgacga ctaccgtcag
     2341 gagcgggagg tcggcgtgaa aatcggcgaa gaaatcctgg aacttcggga tcacgacggt
     2401 catgagcacg gcgatgagcg ccgcggacat gaccatgaga atcgccggat agacgagcgt
     2461 cgaaaccacc ttccgcgaga tggcgatgac tgtcttctgg tacttcaggt accggcgcag
     2521 gacggcttcg atctcccccg acttttctcc cgccttgagc gaggtccagt acaggcgcgg
     2581 aaagatgtcg ccctgctcgg cgaacgcatc ggagatcgac gcgccggcgt tgacgcgatc
     2641 cttcacgtcg gcgagcacgg cgcgaagcgc gggactctcc tgccgctcga gcaggatctc
     2701 gaacgaccgg acgaccggaa ggcccgcctt gaggagcgcg atcatctcct ggttgaacac
     2761 aaggaagtcc gccatcttga ctcgtccctt gcggtccccg cggccgcccc gcaaggcgat
     2821 gccgtggcgg cgcacttcga agacgtgccc gccgcggcgt tcgatctccg cctgcgcggt
     2881 cttcgagtcg ggtgcgagga tcgactgctc ggctacggaa ccgtcgggcc accccgcctt
     2941 gaccgagaac tgcgggctca cggctttttc tccacgcttc tctgaccttc ggttcccttc
     3001 tcggcgccga cgcggggcgt ctcctgcgcg agaagaatca ggatgagggc gttcttcgaa
     3061 ccctcctcct tggaagctcc gacgacgagc gtctgattca acgtcacggc catcggcgcc
     3121 cggtagagcg ccaccggaac gacgcgaccc tgtgcattgc gccgcagccg cgagagcgag
     3181 aaaggcgaca gcatgagctg ctgttcggcc gctccggccg gctcgatcca gaagttcagc
     3241 atgtacgcgt cgccctcctc cccgagctgg gcggcgaccg gccccccctc gatcccgcgc
     3301 atgacggcgg agtcgatcag gctgtagtcg ttgaactgga agagggactt cagcttggcc
     3361 cccatgccgc cgatctcccg atcgatcgag ccggcgggga cctccgcgcg ggcgcgcacg
     3421 agcttgatgg cgaacgtgaa gctgcgcggg ggtgcgtcga actgcgagat cacccgcgcg
     3481 atcgtgcgca ggttctcttc gcgatcggtg accgtgatcg ccctcaacct cggcgtcaga
     3541 gtgacggtgc ccgcatcgga cagatggggc cggatcagga gcgcggcctc ctcgacctgg
     3601 cggtacttca gaatgaagac cttcaccgcg gcttcttccg ccccgaaaat cgcgggcgcg
     3661 aagagcgaga aggccccgat ggccgcggcg aaaatcttct ttctcacgcg aataaaaacc
     3721 ccgctctccg atggagagct accagctgtc agacttcagc ttccgccttg tggccggagg
     3781 ctgcgaacga gttctaaata tcgagcgagc gatccacgat ccacacgacg cgcggctcct
     3841 ggctcgccgc accgggattc cagtcgtaaa tcgtcgcgtc cgcgggaaaa gtcgccgaag
     3901 gagctctttc gctcgccggc gagaaaccct tcgccggcgc gagatccgcg ccgggcgcgg
     3961 gggcggaggc ggaggcggtc agcggcgccg agaccgtgtc gtcgggccgg cggccggacc
     4021 cgggcagcac gagcgtcatc gcggcaagcg ccgccgccga cgccatcgcg gccacgcgcc
     4081 gccggcttcc ccggcgcgca cgccccacac ggctctccgt ccggacgaga gcaattcccg
     4141 tgcgcacgcc ggcgagtact cgcgccacgt cctcggccga gacctcttcc ggtccaacgc
     4201 gcgagaagag gagcgaaggc tcgacgccgg caacacgatc ccggcacgcc ggacaggacg
     4261 caaggtgctc ccggaattca cggcggtcgt ccgccgtgag cgcgtccgcc aggtacggcg
     4321 aaatccgctc gcgaaccgtt ccgcaggagg cgctcacgaa cgtctcccat cgagcaggcc
     4381 gggacagatc ttcgcgatct cccgacgcag gatccgccgc gcgttgaaaa ggtgattgcg
     4441 cacggtggac tcgccgcagt tcaggatcga ggcgatctcc ttcgtatccc ggtcctccat
     4501 ctccttcagc acgaaaacgg ctttctgctt ccccgtcagc cgcgcggcga ccacctggaa
     4561 gagccgcgcg agctcgccgt cctcgacggc gttcatcgcc tccgagccgg tcatctcttc
     4621 gcggaggcgc acgagatgga gcgtcgcgcc gtgagcgcgc tcgcggctgc gggagctgcg
     4681 caggaagtcg atcgagagat tcgtggcgat gcggtagagc caggtgttga acgagtactt
     4741 ctcgtcgtag cgggagatct gctcccagac gcggaggaag acgagctggg cgacgtcccg
     4801 cgcgtcctcg ccgtcgccga cgacccgccg ggcgaccgaa acgaccgccg gcgtcttgcg
     4861 gaccacgagc gcctcgaacg acaccatgtc gcccccccga gcccgcacgg cgagctcggt
     4921 atcggaaagc tcggcggctt cccgtcggtc ccggctttcg ctgtaaacga gctccatcgt
     4981 catgtctctg cggcgggtgc aggcccgcag ggcctgtccc cgcaacattt ccagatcctc
     5041 ccggccgtta tacggagcgg gaacgcttcg cgtcgcccgc tttcttccgg ggggttcctg
     5101 ttcggccgac tcctttggac tgcgacgggt gcaggcccat ggggcctgtc cccgtgacac
     5161 tcccaaacct ttgaatggta gaacgttgtg gcgacaacgg caaggaggat cgctcctctg
     5221 gtgcagcata atcgccgtgg catgctcttc taccggatac tcgcgaccgg cgcgctcctc
     5281 gcctactccc ccgtcgcgct cgtcaaggcg gtcaccggcc gccgccgccc cggggacgtc
     5341 cggggtcggc tgggactctc cccctacccc gacctcgcgg ggggaatctg gatccacgcc
     5401 gtatccgtgg gggaggtcgg ggtggcggtc cccctcctcg cggccctcaa acggctcgcg
     5461 ccggaccggc gtttcgggct ctccgtgacg acggccgccg gccgcgagct cgcccggcgc
     5521 gtcgcgcctt ccggcgtcga cgtcttcgcc ttcccgttcg acctcgccgc gcccgtcgag
     5581 cgggccctcg cccgggtgcg acccgggctc gtgctgctga ccgagaccga gctgtggccg
     5641 ctctttctca cgcgcgcggc cgagcgcggg attccggttg cgctcgtcaa cggccgcatc
     5701 tcgcagcgat cctttccgag gtaccggatg ctccgccgct ggttcgctcc cgcgctcgac
     5761 cgcgtcgcgc tcttcgtgat gcagagcgag gaggacgcac ggcgtatcga agcgctcggc
     5821 gttgccgcgt cgaagatccg gatgagggga aacatcaaat acgaccggcc ggcggcgccg
     5881 ccttttcgcg acgccgagcg cctgatcgcc gccgcggccg gacgccccct tttcgtcgcc
     5941 gcctcgaccg acgaggccga agaggcgacg atcgtcgctg ccgcaagggc gctcgccccg
     6001 cacgccttga ccgcgatcgc gccgaggcgt ccggagcggt tcgacgaggc cgcgcgaaag
     6061 atcgagggag cgggactctc cgtcctgcgc cgatccgggc cggagcgcgt accggcggac
     6121 gtctacctgc tcgacacgat cggcgagctc gcctcgctct actcgcacgc gaagatcgcg
     6181 ttcatcgggg ggagcctcgt ggcgcgggga ggacataatc cgatcgaggc ctgggccgcc
     6241 ggggtgcccg tcatcgtcgg tcctcacacg gagaactttc gcgaggtcac ggcgaagggc
     6301 gaggagctcg cgatcgcgac ccgaatctcg cgcggcgagg agctcgtgga ctgcgtcgag
     6361 cgagagctcg cggaccgtca cgccctcgag gcccgcggcg ccgccgctcg cgagttcgtc
     6421 tcggccaacc ggggcgcggc ggaggcgacg gccgtggagg tcctcccgct gctcaccggg
     6481 tcgccactgt cgagcggagc ggcgcgttga gaccgacccg gctgcccggc ccggtcgtcc
     6541 gcgccgcgct gcgacccttt tcgtcggcgt atggccgcgc tctcgagacc cgcgcgcggc
     6601 tctaccgttc cggggctctc gcctcggaac gagtctccgc tcccgtgatc tcggtcggca
     6661 acctgacgtt cggcgggacc ggcaagacgc cgttcgtcga gctcctcgcg cgccgcttcc
     6721 tcttcgaagg ccgccgtcca gcggtcgtct cgcggggtta cgggcgcggc tcgaagggcg
     6781 tggtcgtcgt ttccgagggg caaggccctc gggtcggacc gcaggagggg ggggacgaac
     6841 cggtcgcgat cgcgcggaga gtaccgggcg tcgtcgtcgt cgtcggagag aagcgggcgg
     6901 acgccgcacg agccgcgatc gaccacggcg ccgatctcat cctgctcgac gacggtttcc
     6961 agcacctcgc cctgcgacga gacgtcgacc tgttgctctt ggacgctgcc gatccgttcg
     7021 gcggagggag gcttccgccc gacggccggc tccgcgagcc cctctccgcc ctgtcccgcg
     7081 ccgacggggt ggtctttacc cggatcgacc gcggggaacc tcccgccggg gtcgtcgccg
     7141 agctcgcgcg ccggaatcca g
//
LOCUS       DASWAV010000499         3398 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U2665
            k141_584809, whole genome shotgun sequence.
ACCESSION   DASWAV010000499 DASWAV010000000
VERSION     DASWAV010000499.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960316
            Sequence Read Archive: SRR10260007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 3398)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3398)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:47:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,929
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3398
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_584809"
                     /isolate="SMAG_U2665"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.36444 N 132.31889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            117..1259
                     /locus_tag="VF958_10410"
     CDS             117..1259
                     /locus_tag="VF958_10410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9149560.1"
                     /translation="MRGRALLLVALAFAAWGCAGAPVSPPAKSRAPYEAPPAARHQLE
                     ITIDARAARDLLAFLSRPDFQPAEAKTLEELPAIRLALQDSNRTTDLFERDLAAAFAE
                     ETRSTVFDFHSIRTGRARWEQLLAAFSSRESSLARLAADRAAALLPADRPIAARLQVF
                     LSFGLAGLADHLVVVMPDGGEAMIVDLARALGESEAESVDSQLERVARLIAGEAFRQG
                     WRAYRSGSPNWTSRDPGLGVLETLFKVVAEQGPVALFHVDENFFPLSVWLKEPMKRTI
                     ADFNRTAERLVESEKDLEQRMTLAGEITRPDFMQQIAGPSGAFLCDGIIQNSGLDAFR
                     AALAGGPRAFFAAYDEAQQKSRDLIPLSPVIRDRLKSPKPGVPGQK"
     gene            1267..2691
                     /locus_tag="VF958_10415"
     CDS             1267..2691
                     /locus_tag="VF958_10415"
                     /inference="COORDINATES: protein motif:HMM:NF012398.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HEX9149561.1"
                     /translation="MSARDFPLRIGGDEVTTGAWIDVHSPWSGELLARVAKAGPGEVE
                     RAIAAAAGGFEEARALPAYRRAEILRRLREELEGFRGELVATMVAEAGKPVRYVTAEV
                     ERGLTTLRLASEEATRLGGEVVPVDIEPRGEGAFCAVRRFPVGPVAAITPFNFPLNLA
                     LHKVAPALAAGNPVILKPSPRTPLTADILSRAVERSGWPKRAFSLVHADPDVARPLWT
                     DDRIRCVSFTGSDAVGWRIKAEAARKKVILELGGNAAAIVCEDANVEDAASKLATAAF
                     AYAGQVCIKAQRLFVARSVYERFVSALLAETEKIEPSDPAEPSAVIGPMIDLESAERV
                     ASWVEEARQAGAKLLVPPRREGSRLWPLVAADVPAGAKLRDREIFGPVATVEPFDRFE
                     DAIAKANDSPYGLQASVFTRDIGRVLKAFDGLEVGGVIVNEAPTMRIDNFPYGGTKAS
                     GFGREGVRYAIEEMTEPRVLFLKP"
     gene            2775..>3398
                     /locus_tag="VF958_10420"
     CDS             2775..>3398
                     /locus_tag="VF958_10420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015178596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HEX9149562.1"
                     /translation="MRAALPENEEARLVALHELGILDTPPEPKFDDLALIASQICGTP
                     ISMISLIDRDRQWFKSEVGVNQRIGQTPRDVAFCAHAILQSDLTVVPDTLEDPRFSDN
                     PFVTADPKIRFYAAAPLRTSDGHALGTLCVVDRTPRTLTGEQRDALRALGHQVEAQIE
                     LKRRLVRERKEADEALHDKEASVRLLAEQMPAVLWSVDPNLRFTSSMG"
BASE COUNT          531 a         1170 c         1164 g          533 t
ORIGIN      
        1 cgggccatcg cggtggcgac cggacccacc cccgcggaaa agctcgcggt cgagcggccg
       61 ggcgctctgt tcgccgactt ctccgacgtc gaagcggcag cgggcgcgat cctgcgatga
      121 ggggccgcgc gcttctgctc gtcgctctcg ctttcgcggc ctggggctgc gcgggagcgc
      181 ccgtttcccc gccggcgaag agccgcgcac cctacgaggc gccgccggcg gcccgccacc
      241 agcttgagat cacgatcgat gcgcgcgccg cccgggacct tctggcattc ctgtcgcggc
      301 cggactttca acccgctgaa gcgaagaccc tcgaggaact ccccgccatc cggctggcgc
      361 tccaggactc gaaccggacg acggacctgt tcgagcgaga cctcgccgct gcgtttgcgg
      421 aagagacgcg cagcaccgta ttcgacttcc actcgatccg gaccggccgc gcccggtggg
      481 aacaactcct cgcggcgttt tcgtcgcgcg agagctctct tgcgcggctg gccgcggatc
      541 gcgccgcagc gttgcttccg gcggatcgcc cgatcgcggc gcggctccag gtgtttctct
      601 cgttcgggct cgcgggcctc gcggaccacc tcgtcgtcgt catgccggac ggcggagaag
      661 cgatgatcgt ggacctcgcc cgcgccctcg gggagtccga agccgagtcc gtcgacagcc
      721 agctcgaacg cgtcgcgcgc ctcatcgccg gggaagcgtt ccgccagggg tggcgcgctt
      781 accgctcggg cagcccgaac tggacgagcc gcgatccggg gctcggcgtg ctcgaaacgc
      841 tcttcaaagt cgtggccgag cagggtcccg tcgcgctctt ccacgtggac gagaacttct
      901 ttccgctctc cgtctggttg aaggagccga tgaagcggac gatcgcggac ttcaaccgaa
      961 ccgcggagag gttggtcgag tcggaaaagg acctcgagca gcggatgacg ctcgccggcg
     1021 agatcacgcg acccgacttc atgcagcaga tcgcggggcc gtccggcgcc ttcctctgcg
     1081 acgggatcat ccagaactct gggctcgacg cgttccgggc agccctggcg ggcggaccga
     1141 gagcgttctt cgccgcctac gacgaggcgc aacagaagtc gcgggacctg attcctctgt
     1201 ctcccgtgat ccgcgatcgc ctgaagagtc cgaagcccgg agtccccggt cagaagtagt
     1261 gcgaccatga gcgcgcgcga ctttccgctt cgaatcgggg gggacgaggt cacgacgggc
     1321 gcgtggatcg acgtgcactc cccctggtcc ggcgagctcc tcgcgcgcgt cgcgaaggcg
     1381 ggtcccgggg aagtcgagcg ggcgatcgcg gcagccgcgg gcggattcga ggaggcccgg
     1441 gccctgccgg cgtaccggcg cgcggagatc ctccgtcgac tgagagaaga gctcgagggg
     1501 tttcgcgggg agctggtggc gacgatggtc gcggaagccg gcaagcccgt gcggtacgtg
     1561 acggcggagg tcgaacgggg actcacgacc ctgcgcctcg cttccgagga ggcgacgcgt
     1621 ctcggaggcg aggtcgtgcc cgtcgacatc gagccgcggg gggagggagc cttctgtgcc
     1681 gtccgccgct tcccggtcgg accggtcgcc gccatcacgc cgttcaactt tccgttgaac
     1741 ctcgcgttgc acaaggttgc ccccgcgctc gctgccggca atcccgtgat tctgaagccg
     1801 tcgccgcgca cgccgctgac cgccgacatc ctctcgcggg cggtcgagag atcgggctgg
     1861 ccgaagaggg ctttctcgct cgtccacgcc gatccggatg tcgcgcggcc gctgtggacc
     1921 gacgaccgca tccggtgcgt gagcttcacg ggcagcgacg ccgtcggctg gaggatcaag
     1981 gccgaggccg cgcgaaagaa agtgatcctc gagctcggcg gcaacgctgc cgcgatcgtc
     2041 tgcgaggacg ccaacgtcga ggacgccgcg agcaagctcg cgacggcggc tttcgcatac
     2101 gccgggcagg tctgtatcaa ggcgcagcgt ctcttcgtcg cgcggtccgt ctacgagcgg
     2161 ttcgtctccg cgttgctcgc cgagacggag aagatcgagc cgtccgatcc ggccgagccc
     2221 tcggccgtca tcgggccgat gatcgacctg gagagcgccg agcgcgtcgc ctcgtgggtg
     2281 gaggaggccc gccaggccgg ggcgaagctc ctcgtgccgc cgcgccgcga aggatcccgt
     2341 ctgtggccgc tcgtcgctgc ggacgttccg gcgggcgcaa agctccgcga tcgcgagatc
     2401 ttcggccccg ttgcgacggt cgagccgttc gaccgcttcg aggacgcgat cgcgaaggcg
     2461 aacgacagtc cctacggcct ccaggcgtcg gtgttcacac gcgacatcgg acgcgtcctg
     2521 aaggcgttcg atgggctgga ggtcggtgga gtgatcgtca acgaggcgcc gacgatgcgc
     2581 atcgacaact tcccgtacgg aggcacgaag gcatccggct ttggccgcga gggagtgcgc
     2641 tacgcgatcg aggagatgac cgagccccgc gtgctcttcc tgaagcctta gagcggcaag
     2701 acattagcgt cgggctgaca gtcgggaccg gccgaaatag gattgcgttc aaggccccga
     2761 caggggagtc atccatgcgc gccgctcttc ccgagaacga agaagcccgg ctcgtggcgc
     2821 tccacgagct cgggattctc gacacgcctc ccgagcccaa attcgacgac ctcgcgttga
     2881 tcgcttctca gatctgcggt accccgatct cgatgatctc gctcatcgac cgcgaccgtc
     2941 agtggttcaa gtcggaagtc ggcgtcaacc agcgcatcgg tcagaccccg cgcgacgtcg
     3001 ccttctgcgc gcacgcgatt ctccagagcg atctcaccgt cgtgcccgac acgctggaag
     3061 acccgcgctt ttcggacaat cctttcgtca ccgccgaccc caaaatccgg ttctacgccg
     3121 cagctccact gcgcacttcc gacgggcacg cgctcgggac tctctgcgtc gtcgaccgga
     3181 cgccgcggac cctgacggga gagcagcggg acgccctgcg tgcgctcggg caccaggtcg
     3241 aggcgcagat cgagctcaag cgacgtctcg tccgcgagcg caaagaagcg gacgaagcgc
     3301 ttcacgacaa ggaagcgagc gtgcgactcc tcgccgagca gatgccagcg gtcctctggt
     3361 cggtcgatcc gaacctccgc ttcacttcgt cgatgggc
//
LOCUS       DASWAV010000500         4191 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U2665
            k141_149744, whole genome shotgun sequence.
ACCESSION   DASWAV010000500 DASWAV010000000
VERSION     DASWAV010000500.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960316
            Sequence Read Archive: SRR10260007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 4191)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4191)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:47:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,929
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4191
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_149744"
                     /isolate="SMAG_U2665"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.36444 N 132.31889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            135..3014
                     /locus_tag="VF958_10425"
     CDS             135..3014
                     /locus_tag="VF958_10425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011421575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HEX9149563.1"
                     /translation="MSASRILTAVCAVSLAAAVTASGEQKTPRRSVPAPSRSAGSADV
                     MPFRAVEKTLANGLKVIVVPTGFPNIVSLQIPVQTGSRNEVEPGKSGFAHFFEHMMFR
                     GTKAYPPDRYNDIITRAGARQNAYTTDDYTNYHTTFSKDDLETILKIEADRFKNLDYS
                     QEGFKTESRAVLGEYNKNSANPIVKIEEVQHDKAFTTHTYKHTTMGFLKDIEDMPNQF
                     DYSRTFFDRWYRPEYTTVIVAGDVDPQRTIALVEKYWGDWKRGSYKVDIPKEPEPKAA
                     VYAHVPWPSPTLPWVTVAFHGPAFSESSKDFAALDLLMDLYFGETSDTYKRLVEQEQK
                     VDGLFTDNPGNADPSLTGVYARVKKMDDALSVRDDLLKTFARARSVAVDAKRLADAKS
                     NARYGLVRSLDNTERIAGTLARYVRYRRSYDTLNNLFRVYQSLTPDDLTAAARRYFTD
                     NRLVVTTLSKDPMPAAVQTLPALATVAPATAAAAFDLPVVLQKTVLPQVNFKLLFTVG
                     SADDPAGKEGLAALAAAMIAEAGSKGMRIDEINKALYPIAGSFRDRVDKEMTTFTGSI
                     HRDNWKAFLDVVLPQLLDPGLREDDFRRLKDAQMNALKEDLRSNNEEELGKERLQTNV
                     FAGTPYGHPVVGTVAGIEAITLDDVRRFISSAYSRANLTLGVAGNVPDEMLARLKEEA
                     SKLPEGKKSPAREVVAHTPSGLEVEIIEKDTRATAISLGHPIGVTRSHPDFPALSVAR
                     AWLGEHRSSVSHLYNRIRETRGMNYGDYAYIEAFPRGMFQFFPDPNIARRAQLFEVWI
                     RPVVPANAHMALRIAVHELDKLVRDGVSKEDFERTRDYLMKNVYVMTATQDQQLGYAL
                     DSRWYGIGEFTSYMRDRLAKLTLEDVNAAIRKHLSSKNLSVVIITKDAKGMKDELVTD
                     AFSPISYDGDKPADLLAEDKLIGAMKLAIPAEKVKITPVEGVFSK"
     gene            complement(3108..3593)
                     /locus_tag="VF958_10430"
     CDS             complement(3108..3593)
                     /locus_tag="VF958_10430"
                     /EC_number="1.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013756.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016646 - oxidoreductase activity, acting
                     on the CH-NH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HEX9149564.1"
                     /translation="MDEDAKRTLLRRIPYGMYVMTSIAEGRPSASTLTWLSQCSFHPP
                     LLMIGVQSNSRMHYAIETSGALAVHFLAEGQENVAQRFFQPPPEEDRHRLHGMPYEPG
                     PVTGVPLLVDLPAWIEARVTDRVDRGDHTLFVCEVVGAGLRDPDFGPLLLSSTPWSYG
                     G"
     gene            3619..>4191
                     /locus_tag="VF958_10435"
     CDS             3619..>4191
                     /locus_tag="VF958_10435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020735609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEX9149565.1"
                     /translation="MSEPHVVVVGAGFGGLTAVRELARAPVQVTLVDRRNHHLFQPML
                     YQVATAGLSPADIAYPVRSILRRQRNASVLLAEATSVDRGARELVLSDGSLRFDYLVL
                     ATGARHAYFGHDAWEKHAPGLKSLEDALDIRRRILLAFERAEREMDESLRRGLLTFVV
                     VGGGPTGVELAGAIGEISREVLVSDFRTIDP"
BASE COUNT          772 a         1428 c         1293 g          698 t
ORIGIN      
        1 ctttcgcgcg aagagaaatc cgaatcaggt atccctattc caccacagtt cgattcttcc
       61 ggcggacgcg atttcccctg ccggctccgg ccgttagaat cactcccttt ggccacgatc
      121 gaaaaggagc ctccatgtcc gcatcccgaa tcctcaccgc ggtttgcgcg gtgtccctcg
      181 ctgccgccgt gaccgcgtcc ggggagcaga agaccccccg gaggtccgtc cccgcgccct
      241 ctcgctcggc gggctccgcg gacgtgatgc cgtttcgggc ggtcgagaag acgctcgcaa
      301 acgggctgaa ggtcatcgtc gtcccgaccg gtttcccgaa catcgtctcg ctccagattc
      361 cggtgcagac cggctcgcgc aacgaggtcg agcccgggaa gtcgggattc gcgcacttct
      421 tcgagcacat gatgttccgg ggaacgaagg cgtatccccc cgaccgctac aacgacatca
      481 tcacccgggc cggcgcccgg cagaacgctt acacgaccga cgactacacg aactaccaca
      541 cgacgttttc caaggacgac ctcgagacga tcctgaagat cgaggccgat cggttcaaga
      601 acctcgacta ctcgcaggaa gggttcaaga ccgagtcgcg cgcggtgctc ggcgaataca
      661 acaagaacag cgcgaatccc atcgtgaaga tcgaggaagt ccagcacgac aaggccttca
      721 cgacccacac ctacaagcac acgacgatgg gcttcctcaa ggacatcgag gacatgccga
      781 accagttcga ctattcgcgg acgttcttcg accgctggta ccggccggaa tacacgacgg
      841 tcatcgtcgc gggcgacgtc gatccgcagc ggacgatcgc actcgtcgag aagtactggg
      901 gcgactggaa gcgcggcagc tacaaggtcg acatcccgaa ggagcccgag ccgaaggccg
      961 ccgtctacgc acacgtgccg tggccgagcc ccacgctccc ctgggtgacg gtggccttcc
     1021 acgggccggc gttctccgag tcgagcaagg acttcgcggc cctcgacctg ctgatggacc
     1081 tgtacttcgg cgagacgtcc gacacataca agcgtctggt cgagcaggag cagaaggtgg
     1141 acgggctctt caccgacaac cccgggaatg ccgatccgtc actgaccggc gtgtacgcgc
     1201 gcgtgaagaa gatggacgac gccctctccg tccgcgacga tctcctgaag acgttcgcgc
     1261 gtgcgcgttc ggtcgcggtc gacgcgaaac ggctcgcgga cgccaagtcg aacgcgcggt
     1321 acggcctcgt gcgttcgctc gacaataccg agcgcatcgc cgggacgctc gcccgctacg
     1381 ttcggtaccg ccgctcctac gacaccctga ataacctgtt ccgcgtgtac cagtctttga
     1441 ctccggacga cctgaccgcc gccgcgcgcc gctatttcac ggacaaccgc ctcgtcgtga
     1501 cgacgctctc gaaggacccg atgccggcgg cggtacagac actgccggcg ctggcgacgg
     1561 tggcaccggc gaccgcggcg gccgcgttcg acctgcccgt cgtccttcag aagaccgtcc
     1621 tgccacaggt gaacttcaag ctcctcttca ccgtcggatc ggccgacgac cccgcgggca
     1681 aggagggcct cgccgcgctc gccgccgcca tgatcgccga ggccggctcg aaggggatgc
     1741 ggatcgacga aatcaacaag gcgctctatc cgatcgccgg gagcttccgc gaccgggtcg
     1801 acaaggagat gacgaccttc accggctcga tccaccgcga caactggaag gcgtttctgg
     1861 acgtcgtgct gccgcagctc ctggacccgg ggcttcgcga ggacgacttc cggcgcctga
     1921 aggacgcgca gatgaacgcg ctcaaggagg atctccgctc gaacaacgag gaggagctcg
     1981 gcaaggagcg gctccagacg aacgtcttcg cgggcacccc ttacggccat ccggtggtcg
     2041 gcacggtcgc cggcatcgag gcgatcaccc tggacgacgt ccgcaggttc atctcctccg
     2101 cgtactcgcg cgccaacctg accctcggcg tggcgggaaa cgtcccggac gagatgctgg
     2161 cgcggctgaa agaggaggcg tcgaagctgc cggagggaaa gaagtcgccc gcacgcgagg
     2221 tggtagccca cacgccgtcg ggactcgaag tcgaaatcat cgagaaggac acccgcgcga
     2281 ccgcgatctc cctcggtcac cccatcgggg tgacgcgttc ccatcccgat tttccggcgc
     2341 tgtccgtcgc gcgtgcgtgg ctgggcgagc accgctcgtc ggtctcgcac ctctacaacc
     2401 gcatccgcga gacccgcggg atgaactacg gcgactatgc ctacatcgag gcgttcccgc
     2461 gcggcatgtt ccagtttttc ccggacccga acattgctcg ccgcgcacag ctcttcgaag
     2521 tctggatccg cccggtcgtt cccgccaacg cgcacatggc cctccggatc gcggtccacg
     2581 agctcgacaa gctcgttcgc gacggcgtgt cgaaggagga tttcgagcgc acgcgcgact
     2641 acctgatgaa gaacgtctac gtcatgaccg cgacgcagga ccagcagctc ggctacgcgc
     2701 tcgactctcg ctggtacggg atcggcgagt tcacgtcata catgagggac cgactcgcga
     2761 agctgacgct cgaggacgtc aacgcggcga tccggaagca cctctcgtcg aagaacctgt
     2821 cggtcgtcat catcacgaag gacgcgaagg gcatgaaaga cgagctcgtc accgacgcct
     2881 tctcgccgat ctcctacgac ggcgataagc cggcggacct gctcgccgag gacaagttga
     2941 tcggcgcgat gaagctcgcg atcccggccg agaaggtaaa gatcacgccg gtggagggcg
     3001 ttttctcaaa gtgacggtca ggtctccact tgccacttcg cttcgcgaag atggcaagga
     3061 cgctttggcg agtcgaaggc attccatatt cgtgaaggcc tgacccccta tcccccatag
     3121 ctccatggcg ttgaagacag gagcaggggc ccgaaatccg gatcgcggag tcctgctccg
     3181 acgacctcgc atacgaagag ggtgtggtcg ccgcggtcca cccggtccgt gacccgtgcc
     3241 tcgatccacg ccgggagatc gacgaggagc gggactcccg tcaccggccc cggctcgtac
     3301 ggcatcccgt gcagccggtg tcggtcctcc tcgggcggtg gctggaagaa ccgttgcgcc
     3361 acgttttcct ggccttccgc cagaaagtgc acggcgagag cgccgctcgt ttcgatcgcg
     3421 tagtgcatgc ggctgttact ctgcactccg atcatcagga gcggcggatg aaaggagcat
     3481 tgcgaaagcc acgtgagggt cgatgcagac gggcgtccct cggcgatcga cgtcatgacg
     3541 tacattccgt agggaatccg cctcagcagc gtgcgtttcg cgtcttcgtc catttccatc
     3601 ctccgatttc cgacccggat gagtgaacca cacgtcgtcg tggtcggggc aggtttcgga
     3661 ggcctgaccg cggtccggga gctggcccga gctcccgtgc aagtgacgct cgtcgatcgc
     3721 cgcaaccacc atctcttcca gccgatgctc taccaggtcg cgacggccgg tctctccccg
     3781 gccgacatcg cctatcccgt ccgttcgatc ctgcgtcgcc agcgcaacgc ctcggtcctc
     3841 ctcgccgagg cgacatccgt cgaccggggt gcgagagagc tcgtgctctc cgacggaagt
     3901 ctccgcttcg actatctcgt gctcgcgaca ggcgcccggc atgcctattt cggacacgac
     3961 gcctgggaga aacacgctcc cgggctcaag tctctcgagg acgcgctcga catccggcgc
     4021 cggatccttc tcgctttcga gcgcgcggag cgcgagatgg acgagtccct tcggcggggt
     4081 cttctgacct tcgtagtcgt cggcggcggg ccgaccggag tcgagctcgc cggcgcgatc
     4141 ggagagattt ctcgcgaggt cctcgtgagc gacttccgca cgatcgaccc g
//
LOCUS       DASWAV010000501         3844 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U2665
            k141_787213, whole genome shotgun sequence.
ACCESSION   DASWAV010000501 DASWAV010000000
VERSION     DASWAV010000501.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960316
            Sequence Read Archive: SRR10260007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 3844)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3844)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:47:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,929
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3844
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_787213"
                     /isolate="SMAG_U2665"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.36444 N 132.31889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1144
                     /locus_tag="VF958_10440"
     CDS             <1..1144
                     /locus_tag="VF958_10440"
                     /inference="COORDINATES: protein motif:HMM:NF012945.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="bifunctional aspartate kinase/homoserine
                     dehydrogenase I"
                     /protein_id="HEX9149566.1"
                     /translation="GEAQRRIHDAFQLSKIGGGSATASERADVVLLGFGQVGRTLAKL
                     IAKGSRRARLRLVAVVDRGGYVFRPEGLSTRDLEGLASLKISSRSVAEGYRGVRAPAA
                     QSVAALSEHALSRPILVDVTAENTSSLLEKSLAWGFDLVLANKRPLAGPRALGERIRS
                     AARNSGRRIRFEATVGAGLPILDTYRKLVEAGDRVSKIEGSLSGTLGFLLSEVDAGRS
                     FSQSLRAAIEKGYTEPDPRDDLSGADVGRKALILGRLLGFRGEPEDVAIESLVPPRFR
                     RVGLERFLEHLEELDRDWEDRVAAARARHRTLRYLASVTRRRIRVGLVAVPPGSPFAG
                     LTGTDNQVAFTTLRYRSNPLVVQGPGAGLDVTAAGIFNDLAELAGG"
     gene            1141..2073
                     /locus_tag="VF958_10445"
     CDS             1141..2073
                     /locus_tag="VF958_10445"
                     /EC_number="2.7.1.39"
                     /inference="COORDINATES: protein motif:HMM:NF002288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004413 - homoserine kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006566 - threonine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine kinase"
                     /protein_id="HEX9149567.1"
                     /translation="MRRVAAFAPGTVANLGPGLDVLGLAVSGAGDTVTVERSVDRRIR
                     IRSSGHPDVPTDPDRNTAGIAAARVREMAGAMATGLEVMVEKGLPLSGGQGGSAASAV
                     AGAVATNELLGSPFRREDLLEPCVKAEETVSGRHADNVAAALFGGVILVSSLDPAVAT
                     ALLFPEDLLVVLAQPQQLLSTREARSVLPASIARDVALAQAGRVGALVAALATRDWEL
                     LRRAVEDRIAEPARAPLLPGFAEAKAAALEAGALGCSISGSGPSAFAFAVGSESAVRI
                     GEAMVRGYRSRKVEARSRVCSIDARGARVVGSDE"
     gene            2136..3368
                     /gene="thrC"
                     /locus_tag="VF958_10450"
     CDS             2136..3368
                     /gene="thrC"
                     /locus_tag="VF958_10450"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014453066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004795 - threonine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HEX9149568.1"
                     /translation="MAFTRSECPECAGLLSVRHAAPGRPGRELRDLFAARRGGRGPDA
                     SGVWRFREMVLPDAEVPPITHPEGNTPLLERDAVARWAGIGQLFLKHEGHNPTGSFKD
                     RGMTVGITQARRVAARAVACASTGNTSASLASYAALAGIPALVLVPAGQVAAGKLAQA
                     LAYGAKTLLVRGDFDACFALVREAAEKLDVYLLNSVNPFRLEGQKTIVLEMLEQLDWE
                     PPDWIALPAGNLGNTSAFGKALSEALDAGLISRVPRLLAVQASGANPFARSFRGGFER
                     RHPVRAETIATAIRIGDPASHDRAVQAIRRTDGVVTDVSDEEILEAKAVIDAAGVGCE
                     PASAASVAGARKLVGQGVVKPGDRVVAVLTGHILKDPGVLLRYHQETVPPPPRANRPV
                     EIEPTLSAVEDVLRRTPR"
     gene            3365..>3844
                     /locus_tag="VF958_10455"
     CDS             3365..>3844
                     /locus_tag="VF958_10455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9149569.1"
                     /translation="MRAKDRGRLAGRPRSAESLIRLHETLLFRRAYPRDRATLVSTEA
                     RLSRFGGRVEAFAKSGGDLEPFDRPEVSGIAGSSITTDYSFDVVRWLAARFPRRVRLD
                     WEDQEDTDRLRSFLPRFLPLLEEEASEDANVPYLEWLCAASPDRRRDLAWLIDRIEAL
                     "
BASE COUNT          528 a         1323 c         1383 g          610 t
ORIGIN      
        1 cggcgaggcg cagcggcgaa tccacgacgc cttccagctt tcgaagatcg gcggaggctc
       61 ggcaaccgcg tccgaacgcg cggacgtcgt gttgctcggc ttcggccagg tcgggcggac
      121 cctggcgaag ctgatcgcga aaggttcgcg gcgggcgcgg ctgcggctgg tggcggtcgt
      181 cgaccgcggg ggatacgtct tccgtcccga agggctttcc acgcgcgacc tcgagggtct
      241 tgcctcgctc aagatttcct ctcgctcggt cgccgaaggc tatcgcggcg tgcgcgcgcc
      301 ggccgcgcag agcgtcgccg cactctccga gcacgccctg tcccggccca tcctggtcga
      361 cgtcactgcc gagaacactt cgtcgctcct cgaaaagtcg ctggcgtggg ggttcgatct
      421 cgtcctcgcg aacaagcgtc ccctggccgg gccgagagca ctgggggagc ggatccggag
      481 cgccgcccgg aacagcggac gccgtatccg attcgaggcg accgtgggcg ccgggctgcc
      541 cattctcgat acctaccgga agctcgtcga ggcgggggac agggtttcga agatcgaggg
      601 ctcgctctcg gggacgctcg gcttcctgtt gagcgaggtc gatgccggtc gctcgttctc
      661 gcagagcctg cgcgcggcga tcgagaaggg ctacaccgag cccgatcccc gggacgatct
      721 ctccggagcg gacgtgggac gcaaagcgct catcctcggc cggctgctcg ggtttcgcgg
      781 cgagccggaa gacgtcgcga tcgagtcgct cgtcccgcca cgctttcgca gggtcgggct
      841 cgaacgtttt ctcgaacacc tcgaggagct cgatcgtgac tgggaggacc gtgtcgcggc
      901 cgcccgcgcc cgacaccgga ccctgcgtta tctcgcctcg gtcacgcggc gccgcattcg
      961 cgtgggtctc gtcgcggtgc cgccgggaag ccccttcgcg gggctgacgg gcaccgacaa
     1021 ccaggtggcg ttcacgactc tccggtaccg gtcgaaccct ctcgtggtcc aggggcccgg
     1081 cgcgggactc gacgttacgg cggcgggcat cttcaacgac ctcgcggagc tcgccggcgg
     1141 atgaggcgcg tcgcggcctt cgcgcccggc acggtcgcca acctggggcc cggcctcgac
     1201 gtgctcggtc ttgccgtgtc gggcgcgggg gacacggtca ccgtcgaacg cagcgtggac
     1261 cggcggatcc gcatccgatc ttccgggcac ccggacgtcc cgacagatcc cgaccggaac
     1321 acggccggga tcgctgcggc gcgcgtccgg gagatggcgg gtgcaatggc gacgggcctg
     1381 gaggtcatgg tcgagaaggg gcttccgctc tccggagggc aggggggcag cgccgcgtcg
     1441 gccgtcgcag gcgcggtcgc gacaaacgag ctcctgggat ctccgttccg ccgtgaagac
     1501 ctcctcgaac cgtgcgtgaa ggcggaggag acggtgtccg gacggcacgc ggacaacgtg
     1561 gccgccgcgc tcttcggggg cgtcatcctc gtgtcgtcgc tcgatcccgc cgtcgcgacg
     1621 gcgctcctct ttccggagga tcttctggtc gtcctcgcac agccccaaca gcttctttcg
     1681 acgcgggaag cccggtcggt tctccccgcc tcgatcgcgc gcgacgtcgc tctcgcgcag
     1741 gcgggaaggg tcggcgccct cgtggccgcc ctcgcgacgc gcgactggga gctccttcgg
     1801 cgcgcggtcg aggatcgaat cgcggaaccg gcccgcgcgc cgcttcttcc cggcttcgcc
     1861 gaagcgaagg cagcggcgct cgaagccggc gccctggggt gttcgatctc cgggagcgga
     1921 ccgtccgcct ttgcgttcgc cgtcggaagc gagtcggcgg tccggatcgg ggaggcgatg
     1981 gtccggggct accgctcacg caaagtggag gcgcgctctc gcgtctgctc catcgacgcc
     2041 cgcggcgcgc gcgttgtcgg gagcgacgag tgacactctc gacgggccgc ccttcgagct
     2101 ggcagttctg tccgcgttgc gcaaccgagg tcgcgatcgc tttcacccgg agcgaatgtc
     2161 ccgaatgcgc gggcctgctc tcggttcgcc acgcggcgcc cggacgcccc ggccgtgagc
     2221 tgcgggatct cttcgccgcg cggcggggcg gacggggacc cgacgcgtca ggcgtgtggc
     2281 gtttccgcga aatggtgctt ccggacgcgg aagttccgcc gatcactcat ccggagggga
     2341 acactcctct tctcgagcgc gacgcggtcg ctcggtgggc gggcatcggg caactcttcc
     2401 tgaagcacga aggccacaac ccgaccggct cgttcaagga tcgggggatg accgtgggaa
     2461 tcacgcaggc gagacgcgtc gcggcacggg cggtcgcgtg cgcctcgacc ggcaacacgt
     2521 ccgcttccct cgcgtcctat gcggcgctgg ccgggatccc cgcgctcgtg ttggtcccgg
     2581 ccggacaggt cgcggcgggc aagctcgcgc aggcgctcgc gtacggggcg aagacccttc
     2641 tcgtccgagg cgacttcgac gcctgcttcg ccctggtgcg cgaggccgcc gagaagctcg
     2701 atgtctacct tctcaactcg gtcaacccgt tccgcctgga aggtcagaag acgatcgtcc
     2761 tcgagatgct ggagcagctc gactgggaac ctcccgactg gatcgcgcta cccgccggca
     2821 acctcgggaa cacgtccgcg ttcggcaagg ccctctccga agctctcgac gccggtttga
     2881 tctcacgcgt gccgcggctt ctcgcggtgc aggcgtcggg tgccaatcct tttgcgcgga
     2941 gcttccgggg cggattcgag cgacggcatc cggtcagggc cgagacgatc gcgacggcga
     3001 tccggatcgg agatcccgcc tcgcacgacc gcgccgtcca agcgatccgc cggacggatg
     3061 gcgtcgtgac cgacgtcagc gacgaggaga tcctcgaggc gaaggccgtg atcgacgcgg
     3121 ccggcgtggg ctgcgagccg gcaagcgccg caagcgtggc gggagcgaga aagctcgtcg
     3181 ggcagggcgt ggtgaagccg ggcgatcgcg tcgtcgcggt gctgacgggt cacatcctga
     3241 aggacccggg agtcctcctg cgatatcacc aggagacggt cccgccgcca ccgcgagcga
     3301 accggcccgt cgagatcgaa ccgactcttt ccgcggtcga ggatgttctc aggaggactc
     3361 cccggtgagg gcgaaggacc ggggacggct ggccggccgg cctcgctcgg cggaatctct
     3421 catccggctt cacgagaccc tgctgtttcg acgcgcgtac ccccgggacc gtgcgacgct
     3481 cgtctcgacc gaggcgcgtc tttcgcgatt cggcgggcgc gtcgaagcgt tcgcgaagtc
     3541 cggcggagac ctcgagccgt tcgaccgtcc ggaggtgtcg gggatcgccg ggagctcgat
     3601 cacgacggac tactccttcg acgtcgtgcg gtggctcgcg gcgcgcttcc cgcggcgcgt
     3661 tcgcctcgac tgggaggacc aggaggacac ggaccggctg cggtcattct tgccccgctt
     3721 tcttccgctt ctcgaggagg aggcgagcga ggacgcgaac gtgccttacc tcgagtggct
     3781 ctgcgcggcg tcgccggacc gacggcgcga cctcgcgtgg ctgatcgacc ggatcgaggc
     3841 gctc
//
LOCUS       DASWAV010000502         5742 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U2665
            k141_67102, whole genome shotgun sequence.
ACCESSION   DASWAV010000502 DASWAV010000000
VERSION     DASWAV010000502.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960316
            Sequence Read Archive: SRR10260007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 5742)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5742)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:47:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,929
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5742
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_67102"
                     /isolate="SMAG_U2665"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.36444 N 132.31889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..830
                     /gene="coaBC"
                     /locus_tag="VF958_10460"
     CDS             <1..830
                     /gene="coaBC"
                     /locus_tag="VF958_10460"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR00521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004632 - phosphopantothenate--cysteine
                     ligase activity [Evidence IEA];
                     GO_function: GO:0004633 - phosphopantothenoylcysteine
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0015939 - pantothenate metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HEX9149570.1"
                     /translation="ATRRNLELLAARGARFVGPGSGFLASGREGAGRMAEPEEIAEAA
                     MALATSARRDLEGLRVLVSAGPTRERLDPIRYVSNRSSGRMGYALAEAARDRGAKVTL
                     VSGPTGLVRPEGMRFVSFETAAELDNLLRAEFPECDVLAMAAAVADFIPEESQERLHR
                     EEGEQRVLLTPGPDLLRGLAPLRRRQTVVAFAAETGDLEPRGRRKMEEKGADLIAVND
                     VGRSDVGFDSPDNEVLLLSRDGGIETVSRRSKREVADRIWDAVRRLRTTSDRPSVLG"
     gene            838..1497
                     /locus_tag="VF958_10465"
     CDS             838..1497
                     /locus_tag="VF958_10465"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011939766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HEX9149571.1"
                     /translation="MPGSTFAHELEFLRDLGYRDFFVRAPGVPELVAGSMRDLPALEA
                     FLAGCPRCKLAATRGRIVFGAGDPNADLMFVGEAPGREEDDQGIPFVGAAGQLLTKII
                     EAMGTSREKVYVVNTVKCRPPNNRNPEPDEIAACRPFFEEQIRLVAPRVIVTLGTFAA
                     QSVLETADPISQLRGRWHVAHGARVMPTFHPAFLLRSPKHKRDVWEDMQKVRDYLAEG
                     R"
     gene            1521..3824
                     /locus_tag="VF958_10470"
     CDS             1521..3824
                     /locus_tag="VF958_10470"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HEX9149572.1"
                     /translation="MPLSPGFRLGPYEIIAPLGAGGMGEVYRARDSRLERDVAIKVLP
                     ESLTADPQALARFEREAKLVAALSHPNILAIHDIGSESGISYAVTELLSGETLRDRLN
                     AGAVPLRKGVGYARQIARGLAAAHARGIVHRDLKPENVFVTSDGTVKILDFGLAKRDL
                     PPPAGGSVEATVARTTPGTILGTVGYMSPEQVRGETADHRSDVFSFGSVLYEIVLGEP
                     AFREETEVETMTAILRHDPPGLRRRGAAPIGLLRVISRCLEKRPEERFQSASDLGFVL
                     EMFAEAETAEGSGEKGPESAPRSPSTPRATISDSSRASIAVLPFRNMSPEKENEYFTD
                     GITEEIINALSKIEALRVASRTSAFAFKGRDEDVRRIGEQLGVRTVLEGSVRRSGTRL
                     RITAELVNVTDGYQLWSERYDREMADVFEIQDEIALAIANTLKVRLLAGEEGALVSRA
                     TDDVEAYNLYLKGKYFFNRRAPRQAITEFEAAIARDPGYAPAHTGLADAYCIFGFYGG
                     IPTLEAFAKARAAASKALEIDPDSPETHVALALVEHYFGWDFDREDREFLRAIELAPR
                     SAAAHSWLALKRTFLDDPAKARPLALKARELEPLSPNVQTNVAWTYFAERRYADAEKE
                     LRRALELDPDALYPLWACALTLQLQGKSEESVALCEKAVALTDRAQTWYLGRLGGSYG
                     AAGRRKDALAVLEELRERSSSEYVAPFHVSFVHMGLGDPDAAIRALELAVEQRNALAW
                     WPKSAPEFDPLRSNPRFVALLERIVPA"
     gene            3863..>5742
                     /locus_tag="VF958_10475"
     CDS             3863..>5742
                     /locus_tag="VF958_10475"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HEX9149573.1"
                     /translation="MTLSAGSRLGPYEIVSAVGAGGMGEVYKARDTRLDRTVAVKVLP
                     ASLSENSAFRQRFEREARAISKLSHPHICTIHDVGNEAGVEYLVMEFLEGETLAERIA
                     KGPLPLDQVLRYGIEISDALEKAHRQGVVHRDLKPANAILTKSGIKLVDFGLAKLDVP
                     AIGSVVSSLSVVPTQADMGLTAQGTILGTFHYMAPEQLEGGEADGRTDIFSLGLLLYE
                     MTTGKKAFTGKSPASLMAAILHMEPEPLSSFQPMTPPALDRIVRTCLAKDPDDRFQTA
                     HDVRLQLQWIVEGGSGVGLPAIVASRRKSREKLAWALVPVAALAAVAATVLVIRLLSE
                     PPRIVRSSLLPPEKSSFAFEFGPMALSPDGSRVAFVATSSGINRLWVRPLSGASAQPL
                     AGTEGASNPFWSPDSRFLGFFANRKLNKIEASGGPPQSLADAPLGRGGSWNREGVILF
                     VPNSRSVVHRVSASGGPVSTVTRLDAGASEVSHRWPVFLPDGRQFLYLVQRGGGADEQ
                     NAIYAASLDGSERKHLFDANSNVVYAPPGYLLFHRERTLLARPFDPRSLRFTEEAFPV
                     AEDVLYFATFAQAAFAASDQGLLAYQTGVSGGETQLTWLDRAGKPTGTVGAPGRLTTP
                     RMS"
BASE COUNT          894 a         2003 c         1924 g          921 t
ORIGIN      
        1 ccgccacacg gcgcaacctc gagctcctcg cggcgcgcgg tgcccgcttc gtcgggcccg
       61 gtagcggatt cctcgcgtcc ggccgggagg gcgcgggtcg catggccgag cccgaggaga
      121 tcgccgaggc ggcgatggcg ctcgcgacct ccgcgcgacg ggacctcgaa gggctccgcg
      181 tcctcgtctc tgccggtccg acccgcgagc ggctcgatcc catccgctac gtgtcgaacc
      241 gttcgagcgg ccggatgggg tacgcgctcg ccgaagcggc gcgcgaccgg ggtgcaaagg
      301 tgaccctcgt ctccggcccc accggtctcg ttcgtccgga ggggatgcgg ttcgtttcct
      361 tcgagacggc cgcagagctc gacaacctgc tgcgtgcgga gttccccgag tgcgacgtcc
      421 tcgcgatggc ggcggcggtc gccgacttca tccctgaaga gagccaggag cggctgcacc
      481 gggaagaggg ggagcagcgc gtgctcctca cgcccggccc cgacctcctc cgcgggctcg
      541 cgccgcttcg ccgcaggcag acggtcgtcg cgttcgccgc cgagacgggg gacctcgagc
      601 cgcgcggacg tcggaagatg gaagagaaag gcgcggacct gatcgcggtc aacgacgtcg
      661 gacggtcgga cgtcgggttc gactcgcccg acaacgaggt gctcctcctc tcgcgcgacg
      721 gcggaatcga aaccgtctcg cgccgctcca agcgcgaggt ggcggaccgg atctgggacg
      781 cggtgcgccg tctccggacc acgagcgatc gaccctcggt actgggctga tcgccgcgtg
      841 cccggctcga ctttcgccca cgagctcgag ttcttgcggg atctcggcta ccgggacttc
      901 ttcgttcgtg cgccgggcgt gcccgagctc gtcgccggtt ccatgcggga cctccccgcg
      961 ctcgaggcct ttctggccgg gtgtccccgg tgcaagctcg ccgcgactcg cggccgcatc
     1021 gtcttcggcg ccggtgatcc gaacgccgac ctgatgttcg tcggagaggc gcccgggcgc
     1081 gaggaggacg accagggcat tcccttcgtc ggcgccgccg ggcagctcct cacgaagatc
     1141 atcgaggcga tggggaccag ccgggagaag gtgtacgtcg tcaacacggt caagtgccgg
     1201 ccaccgaaca accgaaaccc cgaaccggac gagatcgccg cctgccgccc gttcttcgaa
     1261 gagcagatcc gcctcgtggc gccgcgcgtg atcgtgacgc tcgggacctt cgcggcgcag
     1321 tccgtgctcg agaccgcgga cccgatctcg cagctgcggg ggcgctggca cgtcgcgcac
     1381 ggagctcggg tgatgccgac ctttcatccc gcgttcctcc tccgctcgcc caagcacaag
     1441 agggacgtct gggaggacat gcagaaggtc agggactacc tcgccgaagg ccggtgaccc
     1501 ggctcctata atcgcggctc gtgccgctgt ctcccggttt ccgcctcggt ccctacgaga
     1561 tcatcgcgcc gctcggcgcg ggagggatgg gcgaagtcta tcgggcccga gactcgcggc
     1621 tcgagcgcga cgtcgcgatc aaggtccttc ccgagtcgct gaccgcggac ccgcaggccc
     1681 tcgcgcgctt cgagcgggaa gcgaagctcg tcgcggcgct gtcgcatccg aacatactgg
     1741 cgatccacga catcggcagc gagtcgggca tctcctatgc ggtcaccgag ctcttgtccg
     1801 gcgagacgct ccgcgaccgg ctgaacgcgg gcgcggttcc gctccgaaag ggcgtcggat
     1861 acgcccgtca gatcgctcgc ggactcgcgg cggctcacgc acgcggcatc gttcaccgcg
     1921 acttgaagcc cgagaacgtc ttcgtcacgt cggacgggac cgtcaagatc ctcgacttcg
     1981 ggcttgccaa acgggacctg ccgccgccgg ccggcgggag cgtcgaagcg accgtagcgc
     2041 gaacgactcc ggggacgatt ctcggaaccg tcggttacat gtcgcccgaa caggtccgcg
     2101 gcgagacggc ggaccatcgc tccgacgtct tctcgttcgg atccgtgctg tacgagatcg
     2161 tcctcggaga accggcgttc cgggaggaga ccgaggttga aacgatgacg gcgatcctcc
     2221 ggcacgaccc gccggggctg cgtcgccgcg gggccgcgcc gatcgggctg ctccgagtca
     2281 tcagccgttg cctcgagaag cggcccgagg agcgattcca gtcggcgagc gacctcgggt
     2341 tcgtgctgga gatgttcgcc gaggccgaga cggcggaggg ctcgggcgag aagggtccgg
     2401 agagcgcgcc gcgctcgcct tcgaccccgc gagccacgat ctcggactcc tcgcgggcct
     2461 cgatcgcggt gctccctttc cgcaacatga gcccggaaaa ggaaaacgaa tatttcaccg
     2521 acggcatcac cgaggagatc atcaacgcgc tttcgaagat cgaggcgttg agagttgcgt
     2581 cgcgcacgtc ggcgttcgca ttcaaggggc gcgacgagga cgtccggcgc atcggggagc
     2641 agctcggcgt ccgcacggtc ctggaaggga gcgttcgccg ctccggcacg cggctccgga
     2701 tcacggccga gctcgtcaac gtcacagacg gctaccagct ctggtcggag cgctacgacc
     2761 gcgagatggc cgacgtcttc gagatccagg acgagatcgc cctcgcgatc gcgaacaccc
     2821 tgaaggtccg cctcctcgcc ggcgaggagg gggcgctcgt atcgcgcgcg acggacgacg
     2881 tcgaggcgta caacctctac ctgaagggaa agtacttctt caatcggcgt gcgccgcggc
     2941 aggcgatcac ggagttcgag gcggcgattg cccgggaccc cggctacgcc ccggcccaca
     3001 ccggcctcgc ggacgcgtat tgcatcttcg gcttctacgg gggcattccg accctggagg
     3061 cgttcgcgaa agcgcgcgcc gccgcatcga aggcgctcga gatcgacccg gactcaccgg
     3121 agacccacgt cgcgctcgcc ctcgtcgagc actacttcgg atgggatttc gatcgcgaag
     3181 accgagagtt cctccgagcg atcgagctcg ctccgagatc ggcggccgct cactcgtggc
     3241 tggccctcaa gcggaccttc ctggacgatc cggccaaggc tcgcccactc gctctcaaag
     3301 cgcgcgagct cgagccgctc tcacccaacg tccagaccaa cgtcgcgtgg acgtatttcg
     3361 cggagcgtcg ctatgccgac gccgagaaag agctccgccg cgcgctcgag ctcgatccgg
     3421 acgctcttta tccgctctgg gcctgcgccc tgacgctcca gctccagggc aagtccgagg
     3481 agtcggtcgc gctctgcgag aaggccgtcg cgttgacgga ccgggcgcag acctggtacc
     3541 tcggccgcct cggcggcagc tatggagcgg cggggaggcg gaaggacgct ctcgccgtgc
     3601 tcgaagagct ccgcgaacga tcctcgagcg aatacgtcgc acccttccac gtctcattcg
     3661 tccacatggg tctcggggat ccggatgccg cgatccgagc tctcgagctc gccgtcgagc
     3721 agcgaaacgc gctcgcgtgg tggccgaaga gcgcccccga attcgacccg ctgcggtcga
     3781 atccacggtt cgtcgctctc ctcgagagga tcgttcctgc gtaacgcctc gcggctatga
     3841 tttccgcccg gagcatcgcg aaatgactct ttccgccggc tcacggctcg ggccctacga
     3901 gatcgtctcc gccgtcggcg ccggtgggat gggggaggtc tacaaggccc gcgacacgcg
     3961 gctcgaccgc acggtggcgg tgaaggtgct tcccgcgagc ctctccgaga actccgcgtt
     4021 ccgccagcgg ttcgagagag aggcccgggc gatctcgaag ctctctcacc cccacatctg
     4081 cacgatccac gacgtcggca acgaggccgg cgtcgagtac ctcgtcatgg agttcctgga
     4141 aggcgagacg ctggccgagc ggatcgcgaa gggacctctg cctctggacc aggtcttgcg
     4201 ctacggcatc gagatctccg acgctctgga aaaggcgcac cgccaggggg tcgtccaccg
     4261 ggacctgaaa ccggcaaatg ccatactcac gaagtcgggc atcaagctcg tggacttcgg
     4321 gctggcgaag ctcgacgttc ctgccattgg ctccgtcgtc tcgagcctct cggtcgtccc
     4381 cacccaggca gacatgggac tcactgctca gggaacgatc ctcggcacgt ttcattacat
     4441 ggctcccgag cagctcgagg gcggagaagc cgacggtcgc accgacatct tctccctggg
     4501 tctcctcctg tacgagatga cgacggggaa gaaggcattc accgggaaga gcccggcgtc
     4561 gctcatggcc gcgatcctcc acatggagcc ggagcccctt tcttcgtttc agccgatgac
     4621 tcctccggcg ctcgaccgga tcgtccggac ctgcctcgcc aaggacccgg acgaccgctt
     4681 ccagaccgcg cacgacgtcc ggctccagct ccagtggatc gtagaaggcg gttcgggggt
     4741 cgggctgccg gcgatcgtcg cctcgcggag gaagagccgg gagaagctcg cgtgggcgct
     4801 cgtgccggtc gcagcgctcg cggcagtggc ggcgaccgtt ctcgtcattc ggctcttaag
     4861 cgagccaccg cgaatcgttc ggtcctcgct ccttccaccc gagaagtcga gcttcgcgtt
     4921 cgaattcggc ccgatggcgt tgtcaccgga cggcagccgg gtggctttcg tcgcaacgtc
     4981 ttccgggatc aacaggctct gggtccggcc gctctccggg gcctcggccc aaccccttgc
     5041 cgggacggag ggagcttcga atcccttctg gtccccggac agccggttcc tcgggttctt
     5101 cgcgaaccgg aagctgaaca agatcgaggc ttcgggcggc cccccccagt ctctcgcgga
     5161 cgcgccgctc ggcagaggtg ggagctggaa ccgcgaggga gtgatcctgt tcgtcccgaa
     5221 ctcccgctcg gttgtccatc gcgtttcggc ctccggcggt ccggtctcga cggtgacgag
     5281 gctcgacgcc ggtgcgtcgg aggtcagtca ccgctggccc gtcttcctgc cggatggccg
     5341 gcagttcctg tatctcgtcc agcgcggtgg gggggccgac gagcagaacg ccatctacgc
     5401 ggcttctctc gacggcagcg aacggaagca cctcttcgac gccaactcga acgtcgtcta
     5461 cgcgccgccc ggctacctcc tcttccaccg ggagaggacc ctgctcgcgc ggcccttcga
     5521 ccccagatct ctgcgtttca cggaggaagc ctttccggtc gcggaagacg tcctgtactt
     5581 cgcgaccttt gcgcaggcgg cctttgccgc ctcggaccag gggttgctcg cgtaccagac
     5641 gggagtgagc ggaggagaga ctcagctcac ctggctcgac cgggccggca agccgacggg
     5701 caccgtcggc gccccggggc gtctcaccac cccgcggatg tc
//
LOCUS       DASWAV010000503         5431 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U2665
            k141_508839, whole genome shotgun sequence.
ACCESSION   DASWAV010000503 DASWAV010000000
VERSION     DASWAV010000503.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960316
            Sequence Read Archive: SRR10260007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 5431)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5431)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:47:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,929
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5431
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_508839"
                     /isolate="SMAG_U2665"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.36444 N 132.31889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(292..660)
                     /locus_tag="VF958_10480"
     CDS             complement(292..660)
                     /locus_tag="VF958_10480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009835808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX9149574.1"
                     /translation="MKSVDEQPLSGGASSNSSENFDETESLCFGLFEDGRQIGFARVI
                     TDAATFAYLADVYVLGSHRGRGLATWLMECILAHPGLAGLRRWMLVTRDAHALYRKVG
                     FRELAHPERIMEMTFPGIYE"
     gene            688..867
                     /locus_tag="VF958_10485"
     CDS             688..867
                     /locus_tag="VF958_10485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9149575.1"
                     /translation="MILAALIVGAALLMQVPTPFRIFGYPGLAILLFLAAAFGGVWLV
                     VSILVRDREPPPKRK"
     gene            920..2755
                     /locus_tag="VF958_10490"
     CDS             920..2755
                     /locus_tag="VF958_10490"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX9149576.1"
                     /translation="MATAQPSEARSLHPVAAPAAAEPRIHPIVQSDYRVRMVAMLVVG
                     LIPLFHFRGHPPMPALVGMVFTALAWPQLAYFIAKSARDTRAAEFRNLLLDSFFVGGW
                     TAALSFSLLPSICMVAAVHSASLSVGGLRLAARGLVALALGMAVIGFPLGFGVHPESS
                     LGTSLFSIAGILTVTTIFGYFGHLQTRRLRQAKNDLAEHNKQIAEQNRIVEQARQAAL
                     EAKSAAEEANQAKSAFLANMSHELRTPLNAIIGYSEMLEEDAEAAGQKSFVSDLQKIR
                     SSGRHLLGLINDVLDLSKVEAGKMKLFLETFDVANLVDDVIATAQPLVEKTSNRLDVR
                     VASDVGQIREDVTKVRQVLLNLLSNAAKFTEKGTITLDVTREADVTGSWVVFRVRDTG
                     IGMTPEQTVKLFQAFTQADGSTMRKYGGTGLGLALSRKFCVMMGGDINVESVPGKGST
                     FTVRLPGDIENYDGEATSIRVRLPGRRPAHAREAGGAAPSGKVLLCIDGDLAVHELMQ
                     RLATREGFSFLSAANGEEGLKLAREKRPDVVTLEVTLPGIDGWTVMKSLKSDHELSTI
                     PVVMVSISDDQDRGMAMGAVDYLVKPIDRERLAGILAAFRAGVVA"
     gene            2766..3143
                     /locus_tag="VF958_10495"
     CDS             2766..3143
                     /locus_tag="VF958_10495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015195319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX9149577.1"
                     /translation="MAKILLVEDNEMNRDMLSRRLVRSGYEVVMAVQGEEGVALARTV
                     SPDIVLMDMSLPVMDGWEATRLLKGDPATREIPVIALTAHAMSGDREKAREAGCDDFD
                     TKPVEYARLLSKIEGLLQRRKAG"
     gene            3205..3606
                     /locus_tag="VF958_10500"
     CDS             3205..3606
                     /locus_tag="VF958_10500"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF024970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX9149578.1"
                     /translation="MENVVEFRNFVEDACRKAGADDSVCFDLKLVVDEACSNIVVHGY
                     EGLAPGPIGLSFAVSGDEIVITITDQAPPFNPKDAPIPDLDAPASERRPGGLGWYLIR
                     ELVDRIEYEPGRGRGNRLTLVKRFVRRHNLD"
     gene            3616..3954
                     /locus_tag="VF958_10505"
     CDS             3616..3954
                     /locus_tag="VF958_10505"
                     /inference="COORDINATES: protein motif:HMM:TIGR00377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HEX9149579.1"
                     /translation="MMIVVEKREFVTVVSIDGSVDGTTARELVSSFREQVSGGSARLV
                     GNLAGVDYTSSAGLRALLETVKESRLRGGDLRLAAVRPEVLRVLELSGFTSILQVFSD
                     VETAVASFAA"
     gene            3966..4865
                     /locus_tag="VF958_10510"
     CDS             3966..4865
                     /locus_tag="VF958_10510"
                     /inference="COORDINATES: protein motif:HMM:NF013862.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HEX9149580.1"
                     /translation="MGSEVKPLTPTPLPGGEGGRNRVAVVIGSGSVKCAAALGLLKVL
                     DREGIAIDLIVGCSGGSLYAALIALGHSAAAGEEMTRRLWTRELTKRRNYHSFLSAVF
                     PQMFGFSEQFGLVNDALIVERLHAAFGDSVFENARIPLFLTATDFHTGEQAVFSQGSI
                     VDAIRASIAIPYVFRPWRIGDHLYVDGFLSDPMPVGVAIKHGANIIVTMGFESPYQPR
                     ATSVLRFAFQLSSIMSNNLLRANYAFHNLAHHTEILPIIPQFSERIHLFDTDKIPYVI
                     EEGERAAEQQLPYLRRLLAAVES"
     gene            4926..>5431
                     /locus_tag="VF958_10515"
     CDS             4926..>5431
                     /locus_tag="VF958_10515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009556803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX9149581.1"
                     /translation="MNRDLLSRRLKQLGHEVEIAVNGREALEKIAASEFDLVLLDIMM
                     PEVDGYKVLEALRENGTLDQLPVIMISAVTEIDSVVRCLEMGATDYLPKPFNPVLLKA
                     RVGATLEKKRLRDRERLWAKSMERELDIGREIQASFLPEQLPQPAGWEIAARFTPARQ
                     VAGDFYDA"
BASE COUNT          952 a         1786 c         1796 g          897 t
ORIGIN      
        1 cgacgcggcg ggcaagccgg tcgagggcat gggctcgatg aaggtcggag acttctggat
       61 cctgaagttc accgagaagc tccccgcgga cgcgggcctc caggtcgacg tgatgacccc
      121 gaagtcgatg gtgaccgagc gcttcgcgct gaaggacgtc gcgctgccgt aggtcaggtc
      181 ttcactgatc gagagagcgc tcgagaagac gcagcgttct tgccatcatc gcgtagcgat
      241 gtgcaagtgg agacctgacc cccaaagtgg caagtggaga cctgacccca tttactcgta
      301 gatccccggg aacgtcatct ccatgatccg ctccgggtgc gcgagctccc ggaaccccac
      361 cttccgatag agcgcgtgcg cgtcgcgcgt cacgagcatc cagcgacgca gccccgcgag
      421 gcccggatga gcgaggatgc actccatcag ccacgtcgcg agcccgcggc cgcggtgcga
      481 cccgaggacg tacacgtcgg cgaggtaggc gaacgtcgcg gcgtcggtga tcacacgcgc
      541 gaacccgatc tggcgcccgt cctcgaacag cccgaagcag agcgactccg tctcgtcgaa
      601 gttctccgac gagttcgacg acgcgccgcc ggaaagcggc tgctcatcga cggacttcac
      661 aagatcgcca accggatcgc actgggggtg atcctcgcag cgttgatcgt cggcgccgcg
      721 ctgctcatgc aggtcccgac accgttccgc atcttcggct accccgggct tgcgatcctc
      781 ctcttcctcg cagcggcgtt cggaggcgtc tggctcgtcg tgagcattct cgttcgggac
      841 cgcgagccgc ctccgaaacg aaagtaaacg accactgatc gctcgaccgc tgcgctagag
      901 tacgccgcag aaaaaccgca tggcgactgc gcaaccgagc gaagcacgct ctctgcatcc
      961 ggtggccgcg ccggcagcgg cagagccgcg gatccatccg atcgtccaga gcgactaccg
     1021 cgtgcggatg gtcgcgatgc tcgtcgtcgg tctcatcccg ctcttccact tccgcggaca
     1081 ccctcccatg ccggcgctcg tcggcatggt gttcacggcg ctcgcctggc cgcagctcgc
     1141 ctacttcatc gcgaagagcg cccgagacac tcgcgccgcg gagttccgga accttctcct
     1201 cgattcgttt ttcgtcggag gatggaccgc cgctctgtcc ttcagcctcc tcccgagcat
     1261 ctgcatggtg gcggcagtcc actcggcgag tctctccgtc ggaggcctgc ggctcgcggc
     1321 tcggggcctc gtcgcgctcg cgctcgggat ggccgttatc ggatttcctc tcgggttcgg
     1381 ggtccatccg gagtcgagcc tcggcacctc gctcttctcg atcgccggaa tcctgacggt
     1441 gacgacgatc ttcggctact tcggccatct ccagacacgg cggctccgcc aggccaagaa
     1501 cgacctcgcg gagcacaaca agcagattgc cgagcagaac cggatcgtgg aacaggcgcg
     1561 gcaggcggcg ctcgaggcga agagcgccgc ggaggaggcg aaccaggcga agagcgcgtt
     1621 cctcgcgaac atgagccacg agctgcggac gccgctgaac gcaatcatcg ggtacagcga
     1681 gatgctcgaa gaggacgccg aggcggccgg gcagaagtcc ttcgtctccg acctccagaa
     1741 gatccgctct tcggggaggc acctgctcgg attgatcaat gacgtcctcg atctttcgaa
     1801 ggtcgaggcc ggcaagatga agctctttct cgagacgttc gacgtcgcaa acctggtcga
     1861 tgacgtcatc gcgacggcgc agccgctcgt cgagaagaca tcgaaccgtc tcgacgtgcg
     1921 cgtcgcgtcc gacgtcggcc agattcgcga ggacgtgacg aaggtgcggc aggtgctgct
     1981 gaacctcctc tcgaacgcag cgaagttcac cgaaaaggga acgatcacgc tcgacgtgac
     2041 gcgcgaggcc gacgtcacgg gaagctgggt cgtgtttcgg gtgcgcgaca cgggcatcgg
     2101 catgacgccg gagcagacgg tgaagctctt ccaggctttc acgcaggccg acggaagcac
     2161 gatgcgcaag tacggcggga cgggactcgg tctcgccctc tctcggaagt tctgcgtgat
     2221 gatgggcggc gacatcaacg tcgaaagcgt ccccggaaaa gggtcgacgt tcacggtccg
     2281 actgccgggc gacatcgaga actacgatgg cgaggccacg tcgatccgcg tccggcttcc
     2341 cggccggcgc cccgcgcacg cgcgcgaggc ggggggggcg gcgccgagcg gcaaagtgct
     2401 cctctgcatc gacggagacc tcgctgtcca cgagctgatg cagagactcg cgactcgaga
     2461 gggattctcg tttctgtcag cggccaacgg cgaggagggc ctgaaactcg ctcgcgagaa
     2521 gcggccggac gtcgtgaccc tcgaagtcac cctgcccggc atcgacggct ggacggtcat
     2581 gaagtcgctg aaatccgacc acgagctctc gacgatcccc gtcgtcatgg tgtcgatctc
     2641 cgacgaccag gaccgcggga tggcgatggg cgcggtggac tatctcgtca agccgatcga
     2701 ccgcgaacgc ctggcgggga tcctcgcggc cttccgcgcg ggcgtagtcg cctaggcgtt
     2761 tcgcgatggc gaagatcctc ctcgtcgagg acaacgagat gaaccgcgac atgctctcgc
     2821 gccggctcgt ccgcagcggc tacgaggtcg tgatggcggt ccagggggag gagggcgtcg
     2881 cgctcgcgcg gaccgtgtct ccggatatcg tgctgatgga catgagcctg ccggtgatgg
     2941 acggctggga agcgacgcgg cttctcaagg gcgatcccgc cacgcgggag atccccgtca
     3001 tcgctctcac tgcgcatgcg atgtccggag accgggagaa ggcgcgcgag gccggctgcg
     3061 acgatttcga cacgaagccg gtcgagtacg ctcggctgct ctccaagatc gaaggcctgc
     3121 tccagaggcg gaaggctggc tgagctggcg cgacacacgc cttcgggcgg ggcggacaag
     3181 ctcgagatcg agcgtgaggc gcgggtggag aacgtggtcg agtttcgcaa tttcgtcgag
     3241 gacgcctgcc gcaaggcggg cgcggacgat tcggtctgct tcgacctgaa gctcgtcgtc
     3301 gacgaagcct gcagcaacat cgtcgtccac ggttacgagg gtctcgcgcc cgggccgatc
     3361 ggcctctcgt tcgccgtctc cggcgacgag atcgtcatca cgatcaccga ccaggcgccg
     3421 cccttcaacc ccaaggacgc gcccataccg gacctcgatg ccccggcgag cgagcgccgg
     3481 ccgggcggcc tgggctggta tctcatccgt gaactcgtgg accggatcga gtacgagccc
     3541 ggccgggggc gcggcaaccg gctcactctg gtcaagcgat tcgtccgacg tcacaatctg
     3601 gactaggaga gaaacatgat gatcgtggtg gagaagcgag agttcgtgac ggtcgtctcg
     3661 atcgacggca gcgtcgacgg gacgacggct cgcgaactcg tgagctcgtt ccgggagcag
     3721 gtgtccggcg ggagcgcgag gctcgtcggg aatctcgccg gagtcgacta cacgtcgagc
     3781 gccggcttgc gggcgctcct ggagacggtc aaggagtcgc ggctgcgcgg gggagacctg
     3841 agactggcgg ccgtgcggcc ggaagtcctc cgagttctcg agctctcggg gttcacgagc
     3901 atcctgcagg tcttcagcga cgtggaaacc gccgtcgcga gcttcgcggc gtaggggctt
     3961 tcagcgtggg tagtgaagtc aaacccctca cccccacccc tctcccgggg ggagaagggg
     4021 ggagaaaccg cgtcgcggtc gtcatcggct cggggagcgt gaagtgcgcc gctgccctgg
     4081 ggcttctgaa ggtcctcgat cgcgaaggca tcgcgattga tctcatcgtc gggtgcagcg
     4141 gcgggagcct gtacgccgcg ctgatcgctc tcggccacag cgccgcggcc ggcgaggaga
     4201 tgacgcgccg gctctggacc cgcgagctca ccaagcggcg caactaccat tctttcctga
     4261 gcgccgtctt tccgcagatg ttcggttttt ccgagcagtt cggcctcgtg aacgacgcct
     4321 tgatcgtcga gaggcttcac gccgcgttcg gcgacagcgt gttcgagaac gcccgaatcc
     4381 cgctcttcct gaccgcgacc gacttccaca ccggagagca agccgtcttc tcgcagggct
     4441 cgatcgtgga tgccatccgc gcctcgatcg cgatcccgta cgtcttccgg ccatggagga
     4501 tcggcgacca cctgtacgtc gacggcttcc tgtccgaccc gatgcccgtc ggcgtcgcga
     4561 tcaagcacgg cgcgaacatc atcgtcacga tgggtttcga gagcccttat cagccgcgcg
     4621 cgacttccgt tctccggttc gcgttccagc tctcgagcat catgtcgaac aatcttctcc
     4681 gcgcgaacta cgcgttccac aacctggcgc accacacgga aatccttccg atcattccgc
     4741 agttcagcga gcgcatccac ctcttcgaca cggacaagat cccctacgtg atcgaggagg
     4801 gcgagcgcgc cgccgagcaa cagctcccct acctccggcg actgctggcc gccgtcgaga
     4861 gctagaacct gtccgcggag acctcgccgg cgcccgcccg gctcctcgtc gtcgacgacg
     4921 tcgagatgaa ccgcgacctt ctgtcgcggc gactgaagca gctcggccac gaggtcgaga
     4981 tcgccgtgaa cgggcgggaa gcgctcgaga agatcgcggc gtcggagttc gacctcgttc
     5041 tgctcgacat catgatgccc gaggtcgacg gctacaaagt cctcgaggcg ctgcgcgaga
     5101 acgggacact cgaccagctc ccggtgatca tgatttcggc ggtgaccgag atcgacagcg
     5161 tcgtccgctg cctggaaatg ggagcgaccg actatctccc caagccgttc aacccggttc
     5221 tgctgaaggc gcgcgtgggc gccacgctcg agaagaagcg actgcgcgac cgcgagcggc
     5281 tctgggcgaa aagcatggag cgcgagctcg acatcggccg tgagatccag gccagcttcc
     5341 tcccggagca gctcccgcaa ccggcgggct gggagatcgc ggcgcggttt actccggcgc
     5401 gacaggtggc cggcgacttc tatgatgctt t
//
LOCUS       DASWAV010000504         2269 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U2665
            k141_667323, whole genome shotgun sequence.
ACCESSION   DASWAV010000504 DASWAV010000000
VERSION     DASWAV010000504.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960316
            Sequence Read Archive: SRR10260007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2269)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2269)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:47:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,929
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2269
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_667323"
                     /isolate="SMAG_U2665"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.36444 N 132.31889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            179..550
                     /locus_tag="VF958_10520"
     CDS             179..550
                     /locus_tag="VF958_10520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9149582.1"
                     /translation="MRARTVLAAGLLAGLTVTPGCTGRSDGSAATPRSLSAAPATAAA
                     ATPAKAAERDPFRETIRPILSARCGKCHDPGGKMYARLPFDDPNVVSSHSAAVLRRLK
                     GDDRAAMEKWLAGLAPAADRR"
     gene            604..1113
                     /locus_tag="VF958_10525"
     CDS             604..1113
                     /locus_tag="VF958_10525"
                     /inference="COORDINATES: protein motif:HMM:NF024966.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SgcJ/EcaC family oxidoreductase"
                     /protein_id="HEX9149583.1"
                     /translation="MRRIAVRAVVLLSVIASVTPAFAATPKADPARASIEKIGKDFAD
                     AYSRGDAKAVAAFYAEDAIVFPPESDMVKGRDAIQGVWKHALDTGIKSLEFQVVDVIS
                     SGNLAAETGLAVLHVQVMGSQESKVTVKYVVVWKKVGGSWKLYRDIWNSMAPPAAPMA
                     APPPATTPH"
     gene            1298..2065
                     /locus_tag="VF958_10530"
     CDS             1298..2065
                     /locus_tag="VF958_10530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011683957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HEX9149584.1"
                     /translation="MKIRALIADDEPLAREWVRSAVAEDPDLDVIGEAGDGFEAAEAI
                     RRLKPDLVFLDVQMPGLDGFGVLEALSPEEIPAVVFVTAFDQYAVRAFEAQAVDYLMK
                     PFSRDRVEEAVRRVRELVKGRSLEDFRESIAKIVEKVRRDRSYPEWVLLKAEGKNVFV
                     KVRDIDWIESQRNNVRIHVGQTLYLLHETTTAIASRLDPKRFLRIHRSAIVNIERIRE
                     LHPWFNGDYAVVLRDGTQLTLSASYRDRLREFRRFGT"
BASE COUNT          376 a          741 c          776 g          376 t
ORIGIN      
        1 caacccttcc agccatacgt atggacacgg tgcatggggg cacctccttt gacgatggcc
       61 cgatagggcc tttgacgatg gccccgatcg ggccggtaag aaccaccgca aggcgggtgc
      121 cagaggattt actcaaatgg accaagggtc ttacgatccg gggtcggaac cgatgcggat
      181 gcgcgcgaga accgttctgg ctgctggcct cctcgcgggg ctgacggtga cgccagggtg
      241 cacggggcgg tcggacggat ctgccgcgac cccgcggagt ctctcggcgg cgcctgcgac
      301 ggcagccgcc gctactcccg cgaaggccgc ggaaagagat ccgtttcgcg agaccatccg
      361 cccgatcctc tcggcgcgct gtgggaagtg ccacgatccc ggcggaaaga tgtacgcgcg
      421 gctccccttc gacgacccga acgtcgtgtc ctctcactcg gcggcggtgc tgcgccgcct
      481 caagggggac gatcgcgccg cgatggagaa gtggctcgcg gggcttgctc ccgcggccga
      541 ccggcggtag aagtttcccg atttcggcgg gcgcttccgc ccgtcccttt cgaggaggta
      601 ctcatgcgtc gaattgccgt ccgtgccgtc gtcctcttgt ccgtgatcgc aagcgtcact
      661 ccggcgttcg ccgcgacccc gaaggcggac cccgcgcgcg cgagcatcga gaaaatcggg
      721 aaggacttcg ccgacgccta tagccgcggc gacgcgaagg cggtggccgc gttttatgcg
      781 gaggacgcga tcgtgttccc ccccgagagc gacatggtca agggtcgcga cgcgatccag
      841 ggcgtctgga agcacgctct cgacaccggg atcaagtcgc tcgagttcca ggtcgtcgac
      901 gtcatctcga gcggcaacct cgccgcggaa accggactcg ccgtgctcca cgtgcaggtg
      961 atgggatcgc aggaatcgaa ggtgacggtg aagtacgtcg tcgtctggaa gaaggtcgga
     1021 ggctcgtgga agctctaccg cgacatctgg aacagcatgg cgcccccggc ggctccgatg
     1081 gcggcacccc cgcccgcgac gacgccccac tgatcacgca tcgtcgggct gtggcaagtg
     1141 gagacctgac cctcgatgtg gcaagtggag acctgacccc catccccgga aaaatggatc
     1201 tcgtcccggt ttttctggat cacggcagaa cgcgaaccct ccgtctggtc cgttcgtact
     1261 caaatacacg gccccggtgg ccgaacggag gtggccgatg aagatccgcg ccctgatcgc
     1321 tgacgatgag ccgctcgcgc gcgaatgggt gaggagcgcg gtcgcggagg accccgacct
     1381 cgacgtgatc ggcgaggcgg gggacggttt cgaggcggcg gaagcgatcc ggaggttgaa
     1441 gcccgacctc gtgttcttgg acgtccagat gccgggcctc gacggtttcg gcgtcctcga
     1501 ggccctctcg cccgaggaga tcccggcggt ggtgttcgtg accgccttcg atcagtacgc
     1561 cgtgcgcgcg ttcgaggcgc aggcggtgga ctacctgatg aagccgttca gccgcgaccg
     1621 cgtcgaggag gcggtgcgcc gcgtccggga gctcgtgaag gggcgatcgc tcgaggactt
     1681 ccgcgagtcg atcgcgaaga tcgtcgagaa ggtccgtcgc gaccggtcgt acccggagtg
     1741 ggtgctgctc aaggccgagg ggaagaacgt cttcgtcaag gtgcgcgaca tcgactggat
     1801 cgagtcgcag cgcaacaacg tccgcatcca cgttggccaa actctctatc tccttcacga
     1861 gacgacgacg gcgatcgcgt cgcggctcga cccgaagcgt tttctgcgta tccaccgctc
     1921 ggcgatcgtg aacatcgagc ggatccgcga gctccatccg tggttcaacg gcgactacgc
     1981 ggtcgtcctg cgcgacggca cccagctcac gctgtcggcg agctaccgcg accgcctgag
     2041 agagttccgc cgcttcggga cgtgatgagg gtcccctctc tccgctcgag gggagagggt
     2101 tagcggcgtg ccccgcaccg cgcgacaacg ctggacctcc gccgcgtgga tcctcggcgt
     2161 ctggctcttc gtcggaatcc tccaggcgac gcagcgctac ctgcgcgggc gcgagctcga
     2221 gccgagctcg tgggcgttct ggagcgcgct cggagaaaac ctcgtcctc
//
LOCUS       DASWAV010000505         2654 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U2665
            k141_28776, whole genome shotgun sequence.
ACCESSION   DASWAV010000505 DASWAV010000000
VERSION     DASWAV010000505.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960316
            Sequence Read Archive: SRR10260007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2654)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2654)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:47:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,929
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2654
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_28776"
                     /isolate="SMAG_U2665"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.36444 N 132.31889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..126
                     /locus_tag="VF958_10535"
     CDS             <1..126
                     /locus_tag="VF958_10535"
                     /inference="COORDINATES: protein motif:HMM:NF024806.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX9149585.1"
                     /translation="LGQGYLAVGDVDAAIKSYRKSLELDPSNENARNVLKQLKAE"
     gene            complement(139..1041)
                     /locus_tag="VF958_10540"
     CDS             complement(139..1041)
                     /locus_tag="VF958_10540"
                     /inference="COORDINATES: protein motif:HMM:NF022588.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2911 domain-containing protein"
                     /protein_id="HEX9149586.1"
                     /translation="MKKRSAFAPLAWAAVAASAFAQQSPAPAPAAPPPPAVRLPQVSQ
                     GASVKQTIGLNDVTITYSRPGVKGRKIWGGLVPYGSVWRTGANAATSIQFTEDLLVEG
                     QPLPAGTYSLHTIPGEKEWTIIFNKEANQWGSYSYEAAKDALRVKVLSVPGAPQEWMQ
                     FRFEDPSINSARVVLAWENLQVPFTIRNATDTNTKVVAMLRESVAKARPDEWQAYYRA
                     GNYLVQNKISMDEAGAWLDKAIAISPTPQTHYAKARYFDAAGKSREAIAELDRALAAA
                     KADTPSTLVDEIRNVKKTWSEKKS"
     gene            1153..1398
                     /locus_tag="VF958_10545"
     CDS             1153..1398
                     /locus_tag="VF958_10545"
                     /inference="COORDINATES: protein motif:HMM:NF024851.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HEX9149587.1"
                     /translation="MTDSVERKIGSVTVRIDRSTCIGTANCAKVAPEVFVLDDERIIT
                     FREPPTEAPRDRVLDACDVCPVDALSAFDEDGRPLLP"
     gene            1370..1888
                     /locus_tag="VF958_10550"
     CDS             1370..1888
                     /locus_tag="VF958_10550"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX9149588.1"
                     /translation="MKMDGRSSPEAGSPRPLIRPANESDVDAIFRVHGDSIRALCRER
                     YGEREIEAWIAFRPPEAYRSSFASRELFVAEWQGEIVGFGQLDPRRGEIEACYVAPEA
                     IGSGVGSALLERMERQARRHGHAIVRLNATLNAEPFYARMGYRRLGPARHRVSKDVNL
                     ECVRMEKALGGA"
     gene            1885..2349
                     /locus_tag="VF958_10555"
     CDS             1885..2349
                     /locus_tag="VF958_10555"
                     /inference="COORDINATES: protein motif:HMM:NF025160.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HXXEE domain-containing protein"
                     /protein_id="HEX9149589.1"
                     /translation="MSALALDRLTFRFWLLGLAQAAHSIEEMRARLYDFFWVATGRMH
                     EILPAVTQVRMSAQTFALLNMSFIAVLLGSVPFVRARKPAALGLAGIAGAIEVLNGIG
                     HTAGSIWFRRYVPGVATAPFLFVMGVLVLRELFRKPGKVSPLTPTLSRGERG"
     gene            2358..>2654
                     /locus_tag="VF958_10560"
     CDS             2358..>2654
                     /locus_tag="VF958_10560"
                     /inference="COORDINATES: protein motif:HMM:NF012512.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HEX9149590.1"
                     /translation="MIPLDRVRAAAGKIAGKVHRTPLLSAGSLGARVGVELYLKCENL
                     QKTGSFKPRGALNNVLSLSAEERARGLVTVSAGNHAQAVAWAARIAGAACTVVMP"
BASE COUNT          400 a          869 c          902 g          483 t
ORIGIN      
        1 ctcggccagg gatacctggc cgtcggtgac gtcgacgcgg cgatcaagag ctaccgcaag
       61 tctctcgagc tcgacccttc gaacgaaaac gcccgcaacg tgctgaagca gctgaaggcg
      121 gagtgaaacc ggatctcgtc agctcttctt ctcgctccag gtcttcttca cgttccggat
      181 ctcgtcgacg agcgtggagg gggtgtctgc cttcgcggcc gcaagtgccc tgtcgagctc
      241 cgcgattgct tcccgcgact tcccggcggc gtcgaagtac cgtgccttcg cgtagtgggt
      301 ctggggcgtc gggctgatcg cgatggcctt gtccagccac gcgccggctt cgtccatcga
      361 gatcttgttc tggaccaggt agtttccggc gcggtaatac gcctgccact cgtccggcct
      421 ggccttcgcg acgctttcgc gcagcatcgc gacgactttc gtgttcgtgt cggtcgcgtt
      481 ccggatcgtg aacggaacct gcaggttctc ccacgccagt acgactcgcg ccgagttgat
      541 ggaaggatct tcgaagcgga actgcatcca ctcctgcggg gcgcccggaa ccgacaggac
      601 cttcacccgc aaggcgtcct tcgcggcttc gtagctgtaa gaaccccact ggttcgcttc
      661 cttgttgaag atgatcgtcc actccttctc gcccggaatg gtgtggagcg aataggtgcc
      721 cgcgggaaga ggctggcctt cgacaagaag gtcctcggtg aactggatcg aggtggcggc
      781 gttcgcgccg gtcctccaca cgctcccgta cggcacgagg ccgccccaga tcttgcggcc
      841 cttcacgccg ggccgactgt aggtgatcgt cacgtcgttc aacccgatcg tctgcttcac
      901 cgacgcgccc tggctcacct gcgggagccg caccgccggc ggcgggggag cggcaggtgc
      961 cggcgccggc gactgctgcg cgaacgcgga cgctgcgact gcggcccacg cgaggggggc
     1021 gaaagcggat ctcttcttca ttgtcatccc tgtcctccaa tctcagaata tcgcggggcg
     1081 agcgacagga ccgtgacgtc ggcgaccagc cgccaggttc tcgagcgcgc ggatacaatc
     1141 acccgcactg ccgtgacgga ctccgtggag cgaaagatcg gctccgtgac ggtgcggatc
     1201 gatcgctcga cctgcatcgg aaccgccaac tgcgcgaagg tcgcgccgga ggtcttcgtg
     1261 ctcgacgacg agcgaatcat cacgtttcgc gaacctccga cggaggcgcc gcgagaccgg
     1321 gtcctcgatg cgtgcgacgt ctgcccggtc gacgcgctct ccgccttcga tgaagatgga
     1381 cggccgctcc tcccctgagg ccgggagtcc ccggccgctc attcggccgg cgaacgagtc
     1441 ggacgtcgac gccatcttcc gcgtccacgg cgactcgatc cgggcgctgt gtcgcgagcg
     1501 ctacggggag cgagagatcg aagcgtggat cgcgttccgc ccgccggagg cctaccgttc
     1561 ctcgttcgcg tcgcgcgagc tcttcgtcgc ggagtggcag ggagagatcg tggggttcgg
     1621 gcagctcgat ccgcgccgcg gagagatcga ggcgtgctac gtggcgccgg aggcgatcgg
     1681 ctcgggagtc ggatccgcgc tgctcgaacg gatggagcgc caggcgcgcc gtcacggcca
     1741 cgcgatcgtg cgtctgaacg cgacgctgaa cgccgagcct ttctacgcga ggatggggta
     1801 ccgccggctc ggccccgccc ggcatcgcgt ttcgaaggac gtcaatctcg agtgcgtgcg
     1861 tatggagaag gcgctcggag gagcatgagc gcgctcgcgc tggatcggct gacttttcgt
     1921 ttctggctgc tcggactcgc gcaggcggcg cactcgatcg aagagatgcg cgcgcggttg
     1981 tacgacttct tctgggtggc gacggggcgg atgcacgaaa ttctcccagc cgtcacacag
     2041 gtgcggatga gcgcgcagac gttcgcgctg ctcaacatgt cgttcattgc ggtcctcctc
     2101 ggctccgtcc ctttcgttcg agctcgaaag ccggcagcgc tcggcctcgc cgggatcgcc
     2161 ggcgcgatcg aggttctcaa tgggatcgga cacaccgcgg gctcgatctg gttccgccgg
     2221 tacgtcccgg gcgtcgcgac ggcgccgttt ctcttcgtga tgggagtcct cgtgttgaga
     2281 gagctgtttc gcaaacctgg caaagtcagc cccctcaccc cgactctctc ccggggggag
     2341 agggggtagg cggcggcttg attcccctcg atcgggttcg cgccgcggcc gggaagatcg
     2401 cgggaaaggt tcaccgcacg ccgcttctct ccgcgggcag tctcggcgcg cgcgtcgggg
     2461 tcgagctgta cctcaagtgc gagaacctgc agaagaccgg ctcgttcaag ccgcgagggg
     2521 ctctgaacaa tgtgctgtcg ctttccgcgg aggagcgggc gcgggggctc gtaaccgtgt
     2581 ctgccggaaa ccacgcgcaa gccgtcgcgt gggcggcgcg gatcgccggg gccgcctgca
     2641 cggtcgtgat gccc
//
LOCUS       DASWAV010000506         7208 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U2665
            k141_585353, whole genome shotgun sequence.
ACCESSION   DASWAV010000506 DASWAV010000000
VERSION     DASWAV010000506.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960316
            Sequence Read Archive: SRR10260007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 7208)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7208)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:47:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,929
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7208
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_585353"
                     /isolate="SMAG_U2665"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.36444 N 132.31889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..3645
                     /gene="rpoC"
                     /locus_tag="VF958_10565"
     CDS             <1..3645
                     /gene="rpoC"
                     /locus_tag="VF958_10565"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015743125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HEX9149591.1"
                     /translation="GAIKELLQRVDVDMLAEELRMIMKTETSQIKRQKAAKRLKVCDA
                     FRRSGHRPDWMILDVIPVIPPELRPLVPLDGGRFATSDLNDLYRRVINRNNRLKKLLE
                     LRAPEVIVRNEKRMLQEAVDALFDNGRRGRVLKGSNNRPLKSLSDTLKGKQGRFRQNL
                     LGKRVDYSGRSVVVVGPELKLHQAGLPKKMAVELFKPFIYNRLEKKGLAQTIKAAKEL
                     VESGVTEVWDALEEVIRDHPVLLNRAPTLHRLGIQAFQPVLVEGKAIKIHPLVCPAFN
                     ADFDGDQMAVHVPLSPKAQVEAELLMLSSNNILSPASGRPLAVPSQDLVLGIYYLTKS
                     RTNTKGEGRIFSDFDEVILALEQGEVTTHTPIRVRYSGKYIDLTTQYNDQDIVHAEVQ
                     DVERALIDTTVGRVLFNRALPEVVPFINGNLKKRGLSDFVNYVYLTFGEELCVMMLDD
                     LKDLGFNAATRAGISIGVDDMLVPPKKEDYIAGARKEVLEVEGQRNSGVITAGERHNK
                     IIDIWHRTTENISDEMFREMKKSDQVGQEFNPIYIMADSGARGSKEQVRQLAGMRGLM
                     SKPSGEVIETPITANFREGLSVLQYFISTHGARKGLADTALKTADSGYLTRRLVDVAQ
                     DVIVIEEDCGTSDGITVTAIMEGGDTLEPLRDRIVGRVAQEDVYDPLSEEKILAIGEE
                     ITEELASRVQEAGIERVKIRSVLTCETKRGICRLCYGRMLASGKMVEIGEACGVIAAQ
                     SIGEPGTQLTMRTFHYGGTASRVTEQSKHAAKNPGTVRFLSVATVERKDGHLVVVNRN
                     GKITIVDNKGREKERYSVVYGSTIKVSEGQEVVPGQELVEWDPFTSAILTEVGGNVAF
                     RDIVEGENLREETDRVTGLAQNIIVETVGAEKRSPQVLVAGKGAEKKYLLPIGSHLMV
                     TDGQAVSPGDVLAKIPRETTKTKDITGGLPRIVELFEARRPKDAAVITEIDGSVSHGP
                     IAKGMRKVIITGDDLETREYLIPRYTHVNVQEGERVRAGDPLIDGAINPHDILAILGE
                     KELQRYLVDKIQEVYRSQNVAINDKHIEVIVRQMMRSVKIEEVGDTDFLIDEQVDRFR
                     FEEENERVIAAGGQPAIGRPMLLGITKASLSTDSFISAASFQETTRVLTEASISGKVD
                     NLRGLKENVIVGRLIPAGTGMEYYRNVVLEREEIPQVEEPALEDFLTDPAEELALAEA
                     DAEVDASDED"
     gene            3733..4395
                     /locus_tag="VF958_10570"
     CDS             3733..4395
                     /locus_tag="VF958_10570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9149592.1"
                     /translation="MNRFRLLSVVLLCVAVLGCAALLSAEDRPAPPRLSSAVAQAFTD
                     AERETIVSWVMSQAQVKAVIGRHRTRLLRVWSDVAKGNEGPYRRASLILRDYDAGTAR
                     EITVNLSTGGIETRELVGVQPSREEIEEGMSIIRSDPALGAFVANPKLDLLGGFHNRS
                     QRADDPCAREICLDFAFMQANYEGPARYVIVNLTRRVVANRDFRAKPGEASPRMTKKV
                     AP"
     gene            4392..5870
                     /locus_tag="VF958_10575"
     CDS             4392..5870
                     /locus_tag="VF958_10575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9149593.1"
                     /translation="MTARFTARIALVIAGVLAVASGLLAQCNGVNHVTWPAVNPVWDF
                     CWVRPADSSAPNGSGLELTDVRYNGTLILARAHIPLLNVKYTPNVSSCGGANLCYRDW
                     LYSEQDFQCAPCQDASGASASCTALSAVQCTGTTTPATTVCQHPGTDAGSFSGVAVED
                     FGTSLRLTSQCTAGWYRYIPVWEFFPDGTIQARFDATSIDATCVAYTHEHHAYWRLDF
                     DVNGPAGNYVDEVLSGGGAQRVTLERNFIDTSPARSKWRIGSASSPYAVEVARNADDG
                     GAGDANAAVPNDFPIADGWVLAYNASELDDGSNRVGGCGAGLNAYDGNQPVDGADIVL
                     WVRAGALHQGEAGGMAQDCSMFGPTIKVISAGVSGAQRLNTVTPCRIVDTRNAPGPYG
                     GPALPGNGIRDFALAGQCGIPGTAKSVAVNLTVVQPASDGYFTAFAAGGSLPLASMLN
                     FLAGQTKANNAVLPLGTGGAVTIFNGGAGAAHVLIDVTGWFE"
     gene            6097..6471
                     /gene="rpsL"
                     /locus_tag="VF958_10580"
     CDS             6097..6471
                     /gene="rpsL"
                     /locus_tag="VF958_10580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011522025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HEX9149594.1"
                     /translation="MPTISQLVRQGRERVRVKTASPALESSPQKRGVCTRVYTTTPKK
                     PNSALRKVARVRLTNGIEVTTYIPGVGHNLQEHSIVMIRGGRVKDLPGVRYHIIRGTL
                     DAVGVANRKQGRSKYGAKRPKA"
     gene            6486..6956
                     /gene="rpsG"
                     /locus_tag="VF958_10585"
     CDS             6486..6956
                     /gene="rpsG"
                     /locus_tag="VF958_10585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012525971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HEX9149595.1"
                     /translation="MPRRRVVAKREILPDPLYNSQLVTKFVNTVMERGKKSLAESILY
                     GALNKVAEKTQDDPMKAFKKAIENVKPALEVKSRRVGGSTYQVPVEVRPNRRTSLAIR
                     WLIEYAEQRGEKTMREKLAAELLDASNLRGGAVKKKDDTHKMAEANKAFAHYRW"
     gene            6988..>7208
                     /locus_tag="VF958_10590"
     CDS             6988..>7208
                     /locus_tag="VF958_10590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019369671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HEX9149596.1"
                     /translation="MPRNHPLKDTRNIGIMAHIDAGKTTTTERILYYTGVNYKLGEVH
                     EGTATMDWMVQEQERGITITSAATTCFWQA"
BASE COUNT         1356 a         2345 c         2389 g         1118 t
ORIGIN      
        1 ggggccatca aggagctcct ccagcgggtc gacgtcgaca tgctcgccga agagctccgc
       61 atgatcatga aaacggaaac ttcgcagatc aagcgccaga aggcggccaa gcgcctgaag
      121 gtctgcgacg cgttccgccg ctcgggacac cgtcccgact ggatgatcct ggacgtcatc
      181 cccgtgattc cgccggagct gcgccccctc gttccactgg acggcggacg cttcgccacc
      241 tcggacttga atgacctcta ccgccgcgtc atcaaccgga acaatcggtt gaaaaagctc
      301 ctcgagctgc gcgcgcccga ggtcatcgtg aggaacgaga agcggatgct ccaggaggcg
      361 gtggacgccc tcttcgacaa cgggcgtcgc ggccgcgtgc tcaagggctc gaacaaccgg
      421 ccgctgaagt cgctctccga cacgctgaag ggcaagcagg gccgcttccg ccagaatctg
      481 ctcggcaagc gcgtcgacta ttcggggcgt tcggtcgtgg tcgtcggacc ggagctgaag
      541 ctccaccagg cgggtctgcc caagaagatg gcggtggaac tgttcaagcc gttcatctac
      601 aaccggctcg agaagaaggg cctggcccag acgatcaaag cggcaaagga gctcgtcgag
      661 tccggcgtca ccgaggtctg ggacgccctc gaagaggtca tccgcgacca tccggtgttg
      721 ctcaaccggg cgccgaccct tcaccggctg ggcatccagg cattccagcc cgtcctcgtc
      781 gagggcaagg cgatcaagat ccatccgctc gtctgtccgg ccttcaacgc agacttcgac
      841 ggcgaccaga tggcggtcca tgtgccgctg tcgcccaagg cgcaggtcga ggcggaactc
      901 ctgatgttgt cgtccaacaa catcctctcg ccggcgtcgg ggcggccgct cgcggtgccg
      961 tcgcaggacc tggtcctcgg catctactac ctgaccaagt cccggacgaa cacgaagggg
     1021 gaggggcgga tcttctccga cttcgacgag gtcatcctcg ctctcgagca gggggaagtc
     1081 accacccaca cgccgatccg ggtccgctac tcgggcaagt acatcgacct gacgacgcag
     1141 tacaacgacc aggacatcgt ccacgccgaa gtgcaggacg tcgagcgagc gctgatcgac
     1201 accaccgtcg gtcgagtgct cttcaaccgc gcgctcccgg aggtcgttcc gttcatcaac
     1261 gggaacctga agaagcgcgg cctgtccgac ttcgtcaact acgtctacct gacgttcggg
     1321 gaagagctct gcgtgatgat gctcgacgac ctgaaggacc tgggcttcaa cgccgcgacg
     1381 cgggccggca tctcgatcgg cgtcgacgac atgctcgtgc cgcccaagaa agaggactac
     1441 atcgccgggg cccgcaagga agttctcgaa gtggagggcc agcgcaactc gggcgtcatc
     1501 accgccggtg agcgccacaa caagatcatc gacatctggc accgcacgac cgagaacatc
     1561 tccgacgaga tgttccgcga gatgaagaag tccgaccagg tcggccagga gttcaacccg
     1621 atctacatca tggccgactc gggcgcccgc ggatcgaagg agcaggtgcg ccagctggcc
     1681 ggcatgcgcg gactgatgtc gaagccctcg ggcgaagtca tcgagacgcc gatcaccgcg
     1741 aacttccggg aaggcctgtc ggttctccag tacttcatct cgacgcacgg cgcccgcaag
     1801 ggactcgcgg acaccgcatt gaagactgcg gactcggggt acctgacgcg ccgtctcgtc
     1861 gatgtcgctc aggacgtcat cgtcatcgaa gaagactgtg gaacgagcga cggcatcacc
     1921 gtcaccgcga tcatggaggg gggcgacacc ctggagccgt tgcgcgaccg catcgtcggc
     1981 cgcgtcgccc aggaggacgt ctacgacccc ctctcggagg aaaagatcct cgcgatcggc
     2041 gaggagatca ccgaggagct cgcgtcgcgc gtccaggagg cgggcatcga acgggtcaag
     2101 atccgctcgg ttctcacgtg cgaaacgaag cgcggcatct gccggctctg ctacgggcgc
     2161 atgctcgctt ccggcaagat ggtcgagatc ggcgaggcgt gcggcgtcat cgccgcgcag
     2221 tcgatcgggg agccgggcac ccagctgacg atgcggacgt tccactacgg cggaaccgcg
     2281 tcgcgcgtca cggaacagtc gaagcacgcc gcgaagaacc ccgggaccgt gcggttcctc
     2341 tcggtcgcga cggtcgagcg caaggacgga cacctcgtcg tcgtcaaccg caacggaaag
     2401 atcacgatcg tcgacaacaa ggggcgcgag aaggagcgtt attcggtcgt ctacggatcg
     2461 acgatcaagg tttccgaagg acaggaggtc gtgcccgggc aggagctcgt cgagtgggat
     2521 ccgttcacct cggcgatcct gaccgaggtc ggcggcaacg tcgccttccg cgacatcgtc
     2581 gagggggaga acctccgcga ggagaccgac cgggtcaccg gactcgccca gaacatcatc
     2641 gtcgagacgg tcggcgccga gaagcgctcg ccgcaggtcc tcgtcgccgg caagggcgcg
     2701 gagaagaagt acctgctgcc gatcggttcg cacctgatgg tcaccgacgg ccaggcggtc
     2761 tcgcccggcg acgtgctcgc caagattccg cgcgagacca cgaagacgaa ggacatcacg
     2821 ggcggtctgc cgcgcatcgt cgaactcttc gaggcgcggc ggcccaagga tgccgcggtg
     2881 attaccgaga tcgacggttc ggtttcgcac ggcccgatcg ccaagggcat gcgcaaggtc
     2941 atcatcaccg gcgacgatct ggaaacgcgg gagtacctga ttccccggta cacccacgtc
     3001 aacgtgcagg agggggagcg ggtgcgggcg ggcgatccgc tgatcgacgg cgccatcaac
     3061 ccgcacgaca tcctcgcgat cctcggggag aaggaactcc agcggtacct cgtggacaag
     3121 atccaggagg tctaccggtc acagaacgtc gccatcaacg acaagcacat cgaggtcatc
     3181 gtccggcaga tgatgcgcag cgtgaagatc gaggaggtcg gggataccga cttcctgatc
     3241 gacgagcagg tggatcgctt ccggttcgaa gaggagaacg agcgggtcat cgccgccggc
     3301 ggccagccgg cgatcggtcg cccgatgctc ctcggcatca ccaaggcgtc cctgtccacg
     3361 gactcgttca tttcggccgc gtcgttccag gagacgaccc gcgttctcac ggaggcctcg
     3421 atctcgggca aggtcgacaa cctgagaggc ttgaaggaga acgtgatcgt cgggcggctc
     3481 atccccgccg ggaccgggat ggagtactac cgcaacgtgg tgctcgagcg ggaagagatc
     3541 ccgcaggtcg aggagccggc gctcgaagat ttcctgaccg atccggccga ggagctcgca
     3601 ctcgccgagg cggacgccga ggtggatgcc tccgacgagg attagttaca ggctacgagt
     3661 cagaggctgc aggatccggg cccgcggaag cgggcccgga ctttttctgt tagccttcct
     3721 gtccttcatt caatgaatcg attccgactc ctctccgtgg tccttctgtg cgtggcggtg
     3781 ctcgggtgcg cggctctcct ttccgccgag gaccgcccgg cgccgccgcg gctctcgagt
     3841 gcggtcgccc aggccttcac cgatgcggag cgagagacga tcgtgtcctg ggtgatgtcc
     3901 caggcgcagg tgaaggccgt gatcggccga catcgcacgc gcctcctgcg ggtctggtcc
     3961 gacgtggcga aggggaacga ggggccgtat cggcgggcgt cgctgattct ccgcgactac
     4021 gatgcgggta ccgcgcgcga aatcacggtc aacctgtcga ccggcggaat cgagacccgc
     4081 gagctcgtcg gggtccagcc gagccgcgag gaaatcgagg aggggatgtc gatcatccgg
     4141 agcgacccgg cgctcggggc gtttgtcgcg aatccgaagc tcgacctcct cggcgggttc
     4201 cacaaccgga gccagcgcgc ggacgacccc tgcgcgcggg agatctgtct cgacttcgcg
     4261 ttcatgcagg cgaactacga ggggccggca aggtacgtga tcgtcaacct gacccggcgc
     4321 gtcgtcgcga accgcgactt ccgggcgaaa ccgggcgaag cgtctccgcg gatgacgaag
     4381 aaggtcgcac cttgacggct cgtttcactg cccggatcgc tcttgtcatc gcgggagtcc
     4441 tcgcggttgc gtccggcctc ctcgcccagt gcaacggcgt caaccacgtg acgtggccgg
     4501 cggtgaaccc cgtgtgggac ttctgctggg tgcgaccggc ggactcgtcg gcgccgaacg
     4561 gctcgggact cgagttgacc gacgtccgct acaacggaac gctcatcctc gcgcgggcgc
     4621 acatcccgct cctgaacgtg aagtacacgc cgaacgtcag cagctgcggc ggcgcgaatc
     4681 tctgctatcg cgactggctc tattccgagc aggatttcca gtgcgcccct tgccaggacg
     4741 cgagcggagc gagcgcctcc tgcacggcac tctcggctgt tcagtgcacc ggcacgacga
     4801 ctcccgcgac gaccgtctgc cagcatcccg ggaccgacgc gggaagtttc tctggtgtgg
     4861 ccgtcgaaga cttcggcacc agcctgaggc tcacctccca gtgcactgcc gggtggtacc
     4921 gctacatccc ggtgtgggag ttctttcccg acggcacgat ccaggcaagg ttcgacgcga
     4981 cgtcgatcga cgcgacgtgc gtggcgtaca cgcacgaaca tcacgcttac tggcgcctcg
     5041 acttcgacgt caacggcccg gccgggaact acgtcgacga ggtgctctcg ggaggcgggg
     5101 cgcagcgagt gacgctcgag cgcaacttca tcgacacgag tccggcgcgc agcaagtggc
     5161 ggatcggaag cgcgagctcg ccgtatgcgg tcgaggtcgc ccgcaacgcc gacgacggcg
     5221 gggcagggga tgcgaacgcg gcggtcccga acgactttcc gatcgccgac ggctgggtgc
     5281 tcgcctacaa cgcgagcgag ttggacgacg gctccaatcg ggtcggggga tgcggcgccg
     5341 gtttgaacgc gtacgacggc aatcagcccg tcgacggcgc cgacatcgtc ctctgggtgc
     5401 gcgcgggcgc gctccatcag ggcgaagcgg gcgggatggc gcaggactgc tcgatgttcg
     5461 gccccaccat caaggtcatc tctgccggag tttcgggcgc ccagaggctt aacaccgtga
     5521 cgccctgccg catcgtggac acgcgcaatg ccccgggacc ctacggtggc ccggcgcttc
     5581 cgggcaacgg catccgggac ttcgctctgg ccgggcaatg cggcatcccc gggactgcga
     5641 agagcgtcgc ggtcaatctg actgtcgttc agcccgcttc cgacgggtat ttcacggcct
     5701 tcgccgccgg cgggagcctc ccgctcgcga gcatgctcaa cttcctggcc ggacaaacga
     5761 aggcgaacaa tgcggtcctt ccgctcggca cgggaggggc agtgacgatc ttcaatgggg
     5821 gggcgggcgc agcccacgtc ctcatcgacg tcactggctg gttcgagtag caccggaggg
     5881 gggaggagct ccccggtcgg gtctgcaggc ccgcagcgcc tgtccccgcg acggctcccg
     5941 tgcttgacaa ggtcctttgg tttccgtacg attcgcgggt ttttccctcg gactccggcg
     6001 gtactctcaa gagcctccgc cctgtcgggt aaaagcggtt acggtcgatc tcgggccctt
     6061 ccacggctcc gggggcccgg ggtgacagga gagtcgatgc cgacgatttc acaattggtg
     6121 cgacagggac gcgagcgggt gcgcgtgaag accgcgagtc ccgcgctcga gtcctccccg
     6181 cagaagcgcg gcgtctgcac gcgcgtgtac acgaccacgc ccaagaagcc gaactcggct
     6241 ctgcggaagg tcgcccgcgt ccgcctgacg aacgggatcg aggtcacgac ctacatcccg
     6301 ggcgtcggac acaatctcca ggagcactcg atcgtgatga tccgaggcgg tcgcgtgaag
     6361 gacctccccg gcgtccgcta ccacatcatc agggggacgc tcgacgccgt cggcgtcgcc
     6421 aatcgcaaac agggccggtc gaagtacggc gcgaagcgcc cgaaggctta gtaggactta
     6481 ctgatatgcc gagacgtcgc gtcgtcgcca agcgggaaat cctcccggat ccgctctaca
     6541 actcgcagct cgtcacgaag ttcgtgaaca ccgtgatgga gcgcgggaag aagtcgctcg
     6601 ccgagtcgat cctctacgga gcgctgaaca aggtcgccga gaagacgcag gacgatccca
     6661 tgaaggcatt caagaaggcg atcgaaaacg tgaagcccgc gctggaggtc aagtcccggc
     6721 gcgtcggcgg ctcgacgtat caggttcccg tcgaggtgcg cccgaaccgc cgcacgtcgc
     6781 tcgccattcg ctggctgatc gagtacgccg agcagcgcgg tgagaagacg atgagagaga
     6841 agctcgcggc ggagctcctc gatgcgtcga acctccgcgg cggcgccgtg aagaagaagg
     6901 acgataccca caagatggcg gaggcgaaca aagccttcgc gcactaccgc tggtaacccg
     6961 gactcaggac tcaggactta tttcgccatg ccccgcaacc acccgctcaa ggacacccgg
     7021 aacatcggga tcatggcgca catcgacgcc ggcaagacga cgacgaccga gcggatcctc
     7081 tactacacgg gcgtcaacta caagctcggc gaggtccacg agggcacggc gacgatggat
     7141 tggatggtcc aggagcagga gcgcgggatc acgatcacgt ccgccgcgac aacgtgcttc
     7201 tggcaggc
//
LOCUS       DASWAV010000507         1728 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U2665
            k141_550139, whole genome shotgun sequence.
ACCESSION   DASWAV010000507 DASWAV010000000
VERSION     DASWAV010000507.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960316
            Sequence Read Archive: SRR10260007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 1728)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1728)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:47:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,929
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1728
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_550139"
                     /isolate="SMAG_U2665"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.36444 N 132.31889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..307
                     /locus_tag="VF958_10595"
     CDS             <1..307
                     /locus_tag="VF958_10595"
                     /inference="COORDINATES: protein motif:HMM:NF012798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HEX9149597.1"
                     /translation="RGAAGGALWGNLGREWRKARTAAKLPGVRFHDLRHEWASRYMEA
                     GGTPSELMSAGGSRSISQVQRYSKAEKSRIRETLGTLAQGVRTGARIAPVLLEVAGS"
     gene            complement(358..435)
                     /locus_tag="VF958_10600"
     tRNA            complement(358..435)
                     /locus_tag="VF958_10600"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(398..400),aa:Arg,seq:tct)
     gene            complement(479..1438)
                     /locus_tag="VF958_10605"
     CDS             complement(479..1438)
                     /locus_tag="VF958_10605"
                     /inference="COORDINATES: protein motif:HMM:NF015724.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CsgG/HfaB family protein"
                     /protein_id="HEX9149598.1"
                     /translation="MRRLLYITIAIPALLIFATAPLAAADASKPRVAVLEFKNKAEGY
                     AWQWYRAGQAAQDMFVTELVRKGNFRVMEREQLAAIMEEKHLSLSGDIDPKTAVKLGK
                     MIGVEYLITGAVTELGVADRDVNVPSALFGGRMPSVNVRSQKMDAAVDARAFSTSTGE
                     IVWADTASESTSDSSVYVAGAGGGVEDHRKLDRLLRPVVIKLADSLGQTKISTSGLGG
                     ASDASGVAGKIAKAIGSTLYVNIGSEAGVKEGDEFDVSRAGEVIKDPDTGEILGANET
                     RVGRIRINAVMGPRLSKASPISGSIFKVGDTLKSPEAPRANSN"
     gene            1540..>1728
                     /locus_tag="VF958_10610"
     CDS             1540..>1728
                     /locus_tag="VF958_10610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9149599.1"
                     /translation="MRIKRSVLAAIVCSGWLLVGCKARETPGTAAQETAPPPSSTEPA
                     APAAVEVTASSAEAMPTTV"
BASE COUNT          272 a          572 c          575 g          309 t
ORIGIN      
        1 tcggggagcg gcaggcgggg cgctttgggg caacctcggt cgcgaatggc ggaaggccag
       61 gaccgcggcg aagctccccg gcgtgcggtt tcacgatctt cgccacgagt gggcgtctcg
      121 gtacatggaa gccggcggca ccccatccga actcatgtcg gccggcggat cgcggtccat
      181 ctctcaggtg cagaggtact cgaaggcgga gaagtcgcgc attcgggaga ccctcgggac
      241 gctcgctcag ggcgtccgaa caggggcgcg tattgctcca gtattgctgg aggtcgcggg
      301 ctcgtagcca cgcgcgatga tgtgctcagc caacccgctc agaggaccgg cttgaattgg
      361 cctgcccggc aggattcgaa cctgcgaccc acggcttaga aggccgttgc tctgtccggg
      421 ctgagctacg ggcagtccga gagcccatta tcgaacagcc ggagtggcga ctccgcgctc
      481 agttcgaatt cgcgcgaggg gcttccgggc tcttgagcgt gtctccgact ttgaagatgc
      541 tgccggagat ggggctcgct ttggagaggc gcggacccat cacggcgttg atccggatcc
      601 ggccgactct cgtctcgttc gcgccgagga tttctccggt gtccgggtcc ttgatgacct
      661 cgcccgcgcg cgagacgtcg aactcgtcgc cctccttcac ccccgcttcc gatccgatgt
      721 tgacgtagag ggtcgaaccg atcgccttcg cgatcttccc cgcgacgccc gaggcatcgg
      781 acgcgccgcc gaggccgctc gtcgagatct tcgtctggcc gagcgagtcg gcgagtttga
      841 tgacgacggg gcgaaggagg cggtcgagct tcctgtgatc ttcgacgccc ccgccggcgc
      901 cggcgacata aacgctcgag tcggaagtgg attcgctcgc ggtgtcggcc cagacgatct
      961 cgccggtcga cgtcgagaac gcccgcgcgt ccacggccgc gtccatcttc tgactcctca
     1021 cgttcaccga aggcatccgt ccgccgaaga gggcgctggg gacgttgacg tcgcggtccg
     1081 cgaccccgag ctcggtcacc gcgcccgtaa tcaggtactc gacgccgatc atctttccga
     1141 gcttcacggc ggtcttcggg tcgatgtcgc ccgagaggga gaggtgcttc tcctccatga
     1201 tcgcagcgag ctgctcgcgt tccatgacgc ggaagttgcc cttcctcacg agctccgtga
     1261 cgaacatgtc ctgggcggcc tggccggccc gataccactg ccaggcgtag ccctccgcct
     1321 tgttcttgaa ctcgagaacc gcgacgcgcg gcttcgacgc atcggcagcg gcgagcggcg
     1381 ccgtcgcgaa aatcagcaat gccgggatcg cgatggtgat gtagagcaaa cgcctcatgc
     1441 ggtcacctct gagtttggat tgccgcattc taaatccgtt cgggagagcg agacgtcgcg
     1501 cctcgacggg gtatcgttgg ggctcgaagg gggtcgacca tgagaatcaa gcggtccgtc
     1561 ctcgccgcca tcgtctgcag tgggtggctt ctcgtcggtt gcaaggcccg cgagactccc
     1621 ggaaccgccg cccaggaaac tgccccgccg ccttcgagca ccgagcccgc cgcgcccgcg
     1681 gccgtcgagg tcactgcgag ctctgccgag gcgatgccga ccacggtc
//
LOCUS       DASWAV010000508         4121 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U2665
            k141_787810, whole genome shotgun sequence.
ACCESSION   DASWAV010000508 DASWAV010000000
VERSION     DASWAV010000508.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960316
            Sequence Read Archive: SRR10260007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 4121)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4121)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:47:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,929
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4121
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_787810"
                     /isolate="SMAG_U2665"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.36444 N 132.31889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..437)
                     /locus_tag="VF958_10615"
     CDS             complement(<1..437)
                     /locus_tag="VF958_10615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9149600.1"
                     /translation="MRAPSAFRWFAAVSPPPVPLTAVFAVLVTGAAALEMVDRGSSDW
                     VLASIALIQLFAASTGFTRHASRGYYDPVLLGRNRVPIAFAHFAASAAPGVAAWVACG
                     TAQAIAARSASVPAFRPAGWAALLLVSTIPWAASVRVAPLVGGT"
     gene            complement(434..1342)
                     /locus_tag="VF958_10620"
     CDS             complement(434..1342)
                     /locus_tag="VF958_10620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9149601.1"
                     /translation="MFALSLTIASAAAIADGSAGPVGEYGGRWVGGTEIRLLDPSLPA
                     IRRIDRTTLSEKVQPLSGVDASLDPYGLALPGDGTIWVLADKGRFAFRFSEATGERVE
                     KRRLPEPCQGVATFWNHTGFLAVRLRASERMLLRAENGAFRPFSPLVSRSAAGLPEQL
                     IANLLRCGSGTRREIPCWFAAGAAEVFLVGRNGEVRAIPVPSFASPSPARGVSREPGV
                     AFTYPVRDAFLLDGDALWVLSNQDGNRTPLEEGARRGKHLSLVRSGRPERAVALAAEA
                     RAILDATEHALVLLFADGSIRRVVSR"
     gene            complement(1369..1590)
                     /locus_tag="VF958_10625"
     CDS             complement(1369..1590)
                     /locus_tag="VF958_10625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9149602.1"
                     /translation="MTLTRLASPRFLVPTLSLAAVLGSAPPARAANCIQNYAECLVRA
                     ADLDTWWLRSAAGIDCYLDAVACVRAAYF"
     gene            complement(1627..1857)
                     /locus_tag="VF958_10630"
     CDS             complement(1627..1857)
                     /locus_tag="VF958_10630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9149603.1"
                     /translation="MIAATHRRIAKRALRFLPPLAVAPLLIPSPPARAECLLDYVDCV
                     EAASELNTFAKRSLAGLGCYVDLISCLQRRLA"
     gene            1938..3191
                     /locus_tag="VF958_10635"
     CDS             1938..3191
                     /locus_tag="VF958_10635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012632801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HEX9149604.1"
                     /translation="MARVLLLLPTTTYRAADFLEAAGRLGVEVTVASDEPSTMEALNP
                     AGLLALDFRSPEGSARKVSEFARAHPLGAVVGVDEDTAVVAAACSRALGLPSNPPEAA
                     AAARDKALMHRLLDASGVPNPRSRVYRIGDGPEAPAREVAYPCVLKPTFLAASRGVIR
                     ADDPASFRGAWARISAILREPDVARKGGPSANEILVEEFVPGREVAVEGMLSKAELDV
                     LAVFDKPDPLDGPFFEETIYVTPSRLAGPALEEVRSVTSLGCHALGLREGPVHAELRV
                     HDGSASVIELAARSIGGLCSRTLKFGSGMSLEELILRHALAIDDGLPPRDPRAAGVMM
                     IPIPRAGRFDGVFGLDEARAVPNVEDVVISAHPGQLLVPLPEGSRYLGFIFSRAQTPD
                     RAEAALREAHRRLVFDIRPVRTAGG"
     gene            complement(3216..3806)
                     /locus_tag="VF958_10640"
     CDS             complement(3216..3806)
                     /locus_tag="VF958_10640"
                     /inference="COORDINATES: protein motif:HMM:NF013286.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NifU family protein"
                     /protein_id="HEX9149605.1"
                     /translation="MSDEIQIRGEPLGAGESCRFTADRPVLPGEAAFFADAESAKSSP
                     LAADLLALPGVTSVLIAEETVTLDAGYAIDWPALGAGNVIRKHLRAGRPIVSASYFDG
                     LPSESDLKWAIDDLLSREINPAVATHGGWVELIDVKKNNVYLRLGGGCQGCGAADVTL
                     KQGIAKAIRDLAPAVGEILDTTDHAAGRNPYYAPAK"
BASE COUNT          661 a         1384 c         1466 g          610 t
ORIGIN      
        1 gtcccgccga cgagcggtgc cacccgcacg ctcgccgccc agggaatcgt cgagacgaga
       61 aggagagcgg cccaaccggc gggacgaaac gcggggacgg acgcggagcg tgcggcaatc
      121 gcctgagcgg ttccgcaagc gacccacgcg gcgacccccg gcgccgccga cgcggcgaag
      181 tgtgcgaagg caatcggaac ccggttccgt ccgaggagca ccgggtcgta gtagccgcgg
      241 ctggcatgcc gcgtgaaacc ggtcgaagcc gcgaagagct gaatcagcgc gatcgaggcg
      301 agcacccagt cggaggaacc gcggtcgacc atctcgagag cggcggcgcc ggtgacgaga
      361 accgcgaaca ccgcagtgag cggcaccggc gggggcgaaa ccgcggcgaa ccagcggaaa
      421 gcggacggcg ctctcatctc gacacgacgc ggcgaatcga tccgtcggcg aagaggagaa
      481 cgagcgcgtg ctcggtggcg tcgagaatcg cgcgcgcctc cgcggccagg gcgaccgccc
      541 gctccggccg accacttcgc accaacgaga ggtgctttcc ccggcgggcg ccctcctcga
      601 gcggtgttcg attcccgtcc tggttggaga gcacccacag ggcgtcgccg tccaggagga
      661 aggcgtcgcg caccgggtac gtgaaggcga ctccgggctc ccggctgacg cccctcgccg
      721 gcgacggaga tgcgaacgaa ggaaccggga tcgcgcggac ctctccgttg cggccgacga
      781 ggaaaacctc cgctgcgccc gcggcgaacc agcaggggat ttcgcgccgg gttccggaac
      841 cgcaccgcaa gaggtttgcg atgagttgtt cgggtagccc ggctgcggac cgcgagacga
      901 gcggtgagaa aggccggaag gctccgttct cggcccgcag cagcatccgc tcgctcgcgc
      961 gcaggcggac cgcgaggaaa ccggtgtgat tccagaacgt cgcgaccccc tggcagggtt
     1021 cggggaggcg gcgcttctcg acgcgttcgc ccgtcgcctc ggagaaccgg aacgcaaacc
     1081 ggcccttgtc ggcaagcacc cagatcgttc cgtcgccggg cagggcgagg ccataaggat
     1141 cgagcgacgc gtcgacgccg gagaggggct gcaccttctc ggaaagggtc gtccgatcga
     1201 tgcggcgaat cgccgggagc gacgggtcga ggaggcgaat ctcggtgccg ccaacccacc
     1261 gtccgccgta ttcgccgacg ggcccggcgg acccgtcggc gatcgccgcg gcggaagcga
     1321 tggtcaggga aagagcgaac atccgtgcgt cgcgacggct tacggcgcct agaagtaggc
     1381 cgctctcacg cacgcgaccg cgtccaggta gcagtcgatt ccggcggcgc tcctcagcca
     1441 ccacgtgtcg aggtcggcgg cgcggaccag acactcggcg tagttctgga tgcaatttgc
     1501 ggcacgggcg ggcggggcgg agccgagcac ggccgcgagc gagagagtcg ggaccagaaa
     1561 ccggggcgac gccagtcggg tcagagtcat ggcttctcct ttcacgagag agacacgcgt
     1621 ttcggatcag gcgagacggc gctggagaca cgagatcagg tcgacgtagc agccgaggcc
     1681 ggcgagagat cgcttcgcga acgtgttgag ctccgacgcc gcctcgacgc aatccacgta
     1741 atcgaggaga cactcggcgc gggccggggg agaggggatg aggagcgggg ccacagcgag
     1801 cggtgggaga aatcgcagtg cgcgctttgc gatccggcgg tgagtagcgg cgatcatgtc
     1861 gtcgacctcc tgctcaagga atgtcgcggc tcgatcgcgc ggcccgtgct ctcgcgacaa
     1921 cttgacagaa tggggaagtg gcgcgtgtgc ttctcctgct gccgacgacg acataccggg
     1981 cggccgactt cctggaagcc gccggccggc tcggcgtcga ggtcacggtc gcgtccgacg
     2041 agccgagcac gatggaggcg ttgaaccccg cgggtctcct ggcgctcgac tttcgtagtc
     2101 cggaaggctc cgcgcggaag gtgagcgagt tcgcgcgcgc gcacccgctc ggcgcggtcg
     2161 tcggcgtcga cgaggacacc gccgtcgtcg cggccgcgtg ctcgcgcgct ctcggcttgc
     2221 cgagcaatcc tccggaagcc gcggccgccg cccgcgacaa ggcgctgatg caccgcctcc
     2281 tcgacgcttc cggcgttcca aacccccgat ctcgggtgta ccgaatcggc gatggtcccg
     2341 aggctccggc gcgagaggtc gcatacccgt gcgtgctgaa gccgacgttc ctcgccgcga
     2401 gccggggagt gatccgcgcg gacgatccgg cctccttccg cggcgcgtgg gcgcgaatct
     2461 ccgcgatcct gcgcgagccc gacgtggcgc ggaaaggcgg accgtcggcg aacgagatcc
     2521 tcgtcgagga gttcgtcccg gggcgcgagg tcgccgtcga agggatgctc tcgaaggcgg
     2581 agctcgacgt gctcgcggtc ttcgacaagc cggacccgct ggacggcccg ttcttcgagg
     2641 agacgatcta cgtcacgccg tcgcgcctgg ctgggcccgc tctcgaggag gtgcgctcgg
     2701 tgacctcact cggctgccat gccctcggcc tgcgggaggg cccggtccac gccgagctgc
     2761 gagtccacga cggctcggcg tccgtcatcg agctcgccgc ccggtcgatc ggcggcctct
     2821 gctcgcgcac gctcaagttc ggctccggga tgtcgctcga ggagctcatc ctgcggcacg
     2881 ctctcgcaat cgacgacggc ctccctcccc gcgacccgag ggcggcggga gtgatgatga
     2941 tcccgatccc gcgcgcgggc cggttcgatg gtgtattcgg gctcgacgag gcgagggcgg
     3001 tcccgaacgt ggaggacgtc gtcatctccg cgcaccccgg tcagctcctc gtgcccctgc
     3061 ccgaagggtc acgctatctc ggcttcattt tcagtcgagc tcagacgccc gaccgcgccg
     3121 aggcggcgtt gcgcgaagcg caccggaggc tcgtcttcga catacggcct gtgaggaccg
     3181 cgggcggcta gagctcgaga cgattcgtcg ggaacttact tggccggggc gtaatatggg
     3241 ttgcggccgg ctgcgtggtc ggtcgtgtcg aggatctcgc cgaccgcggg cgcaaggtcg
     3301 cggatcgcct tcgcgattcc ctgcttgagg gtgacgtccg cggcgccgca gccctgacac
     3361 ccgccgccga gcctcaggta gacgttgttc ttcttgacgt cgatcaactc gacccacccg
     3421 ccgtgcgtgg caaccgcggg attgatctcg cgcgaaagga ggtcgtcgat cgcccatttc
     3481 agatcggact ccgaaggaag gccgtcgaag tagctcgccg aaacgatcgg tcgtcccgcc
     3541 cgaaggtgct tgcgaatgac gttgccggcg ccgagcgcgg gccagtcgat cgcgtaaccc
     3601 gcgtcgagcg tgaccgtctc ctcggcgatg agaaccgacg tcaccccggg cagcgccagg
     3661 aggtcggcgg cgagaggcga ggacttcgcg ctttccgcat ccgcgaagaa agcggcttcg
     3721 ccggggagca ccgggcggtc ggcggtaaat cggcaactct cgcccgctcc gagaggctcg
     3781 ccgcggatct ggatctcgtc gctcatcccg cggggccagt atagcctccg cttccggtac
     3841 ggcgtttttt gccccccttc gctgaccggc tgacatttcg tgaccctttc gcccgccgcg
     3901 aatcgcacac cgtcagcgta accccctgag gttgtgtcgg atgcgccggt cgtgtccgcg
     3961 ggtacgccgt tcgctcattg accttggaat ggactcccca cgcccgcgtc ctgtctcgtc
     4021 gagcgggttc tcgtcgttcg agctggtggt cgcagcggca atcacggtga gcgtttgcct
     4081 cttcacgttc gcctacatcc acacgatcgt gagacgggag c
//
LOCUS       DASWAV010000509         7971 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U2665
            k141_509363, whole genome shotgun sequence.
ACCESSION   DASWAV010000509 DASWAV010000000
VERSION     DASWAV010000509.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960316
            Sequence Read Archive: SRR10260007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 7971)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7971)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:47:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,929
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7971
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_509363"
                     /isolate="SMAG_U2665"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.36444 N 132.31889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1054
                     /locus_tag="VF958_10645"
     CDS             <1..1054
                     /locus_tag="VF958_10645"
                     /inference="COORDINATES: protein motif:HMM:NF013069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HEX9149606.1"
                     /translation="LTTTVEGRNRFPVRVRYSRELRDDPDSIRRILVPAAGSGGMLPA
                     SAPPAERMEETTTETSGARAPFAGAPNPLGARVQIPLSEVAEIRFRPGPSMISSENGL
                     LRERVFLNVRGRDVGSLVDEAKRVVESKVALPAGYFVEWAGQFEHQLRARNRLMVVVP
                     VCLAIIFLLLYLTYHSIKEAAHVILAIPFALTGGNFLLWLLHRLAVTNGWKTEFHMSV
                     AVWVGYIALFGTAVQTAVVMVVYLEEALQRKAGEGPLTRSSIREAAMEGAVLRLRPKL
                     MTVSTVVLGLLPIMWSTATGSEVMKPIAAPVLGGMLSSLAHVLIVTPVIWTMLKEREL
                     RKGRIEVGKVSDLLKA"
     gene            complement(1068..1685)
                     /locus_tag="VF958_10650"
     CDS             complement(1068..1685)
                     /locus_tag="VF958_10650"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013294108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX9149607.1"
                     /translation="MLSKEHWERIYSTRPTNAVSWYQEHAESSLRLIRATGAPKDAAI
                     IDVGGGASTLVDDLLAAGYSRLSVLDLSGTALAAARKRLGARASAVRWLEADVTKVDL
                     PPNEYDIWHDRAVFHFLTSDEDRAAYVRTVSKSVKPGGHVIVATFAEDGPTECSGLPV
                     VRYGAEQLHSEFGETFTLVSHEKEEHHTPLGKVQQFVYCYCRKGI"
     gene            complement(1699..2301)
                     /locus_tag="VF958_10655"
     CDS             complement(1699..2301)
                     /locus_tag="VF958_10655"
                     /inference="COORDINATES: protein motif:HMM:NF024834.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HEX9149608.1"
                     /translation="MRFLVGVLFGILLVAIAAAAIVYTGSFNVSAIVPPSKTERTLAT
                     FALNKAVARRAPARKNPLAANAETLRGGLAHFRENCVICHGAPGVDPREIGQGLNPGA
                     PDLTLRRVQARSDGELFWITSNGIRMSGMPAFSPTHSENEIWQTVAFLRHLPEITEEE
                     TAALKPEAEETQHHHEEAPPPGGAAKPEHTHAPGTPPHED"
     gene            complement(2322..3245)
                     /locus_tag="VF958_10660"
     CDS             complement(2322..3245)
                     /locus_tag="VF958_10660"
                     /inference="COORDINATES: protein motif:HMM:TIGR01297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HEX9149609.1"
                     /translation="MGSVAANAGSANSALPEPTSRKRRLRAALGVTLSVLFLEVLGGL
                     VSGSLALVADANHMLADVAALTLAYAAAALASRAPTSRHTFGLVRAEVLAAFVNAQIL
                     LLVCVWLLYEGFRRLDDPSVIRLSVMIPVAVVGLAANLVSMAILAPDRRGGLNVRAAY
                     LEVVMDAAGSLAVLAAGLAIALTGRLWFDLAASGLIALFVLPRAVGFLRQSAHILLEG
                     APREIDLPSLREHILALPGVEEAHDFHFWTLTSGSHSASVHIRATADSPRGQVLHDVQ
                     ELLRSEAGVEHATIQVEIGPERDCFTAPDHA"
     gene            complement(3233..5143)
                     /locus_tag="VF958_10665"
     CDS             complement(3233..5143)
                     /locus_tag="VF958_10665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015248914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family metallopeptidase"
                     /protein_id="HEX9149610.1"
                     /translation="MRYEQLAAVVVMSLAAAGCGGPSATPASAATPSATAATRTSRDG
                     HSYGNPEEIRVRHANLDWDVLFEQRILRGRADLLLERPAGGRATRLVLDTRDLDVEKV
                     ETSTDGTVWSDAAFSLAPADKILGSALTVVVPEDATRARVTYATRPQASALQWLDPQQ
                     TAARKNPFLFTQSQAIHARSWIPLQDSPQVRITYRARVRTPKNLLAVMSANNDPRVVR
                     DGEYDFEMTQPIPSYLIALAVGDLAALPVSPRTGVWAEPTVVERAAREFSDLEQMIRA
                     TEELFGPYRWERYDVLVLPPSFPFGGMENPRLTFATPTVLAGDKSLVALVAHELAHSW
                     AGNLVTNATWSDFWLNEGITTYIERRIIERVYGVRRAEMEAVLGRQDLEKEMADLPDK
                     DEILHVDLAGRDPDDGFTDVPYEKGALLMRLLEETFGRQQFDRFLRSYFDHFAFQSIT
                     TEDFVRYLKSNLFDASPELAARIPLEEWIERPGLPAGAPRPRSQAFVAVEAATGRWLE
                     RKAPASALDTRAWSTQEWLHFLRALPLPLDRDRMAELDRTFALTASGNSEITNQWLSI
                     AIASGYEPAYPRLEEFLTSMGRRKFLKPLYEALVKTPEGKARALAIYRKARPTYHPIA
                     AAMVDKIVGWGA"
     gene            complement(5264..6505)
                     /locus_tag="VF958_10670"
     CDS             complement(5264..6505)
                     /locus_tag="VF958_10670"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HEX9149611.1"
                     /translation="MLEDLFAAGPSLVALMLTTRCNFRCAYCYQDRSAPRSMQLCVLE
                     AAVDRFLRSDHSHPTLAFFGGEPLLEMSLVRRAVEIVRAETPTGMTPDLEISTNGVFL
                     DEETARFLVEADVHITLSIDGLPGAQNQRGPGSFDILDRLLVRFGRDHPGHLRTRFAV
                     SVTLSSGNVSYLAGSFRYFVSRGVREIRVVPVFTHDSGWGLDARDELDRQLADVVEES
                     LKVHASSGEVWFQAFQPDGRETSGQAYAPSLCGTAGRKSLFVDMDGSLAPCGAMARSC
                     LRDPPPLLRDAIETLGTFDLTDPDLAEKLAARERKVRDLPLLTGKTAKYSPFGRCGEC
                     PALLNCVICPASIAFSAGAAEPDRIPAIQCDFNRLIEKHRMAFHRAVSRANWPEASEA
                     EHRTRKVRMRSTQPVRPADGS"
     gene            complement(6520..6795)
                     /locus_tag="VF958_10675"
     CDS             complement(6520..6795)
                     /locus_tag="VF958_10675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9149612.1"
                     /translation="MKVEVDEAIKCMELLEALTQPTIHYLNVLKKQTETEFIVELREG
                     PIGAGGPLVKPVRACITEPRPGDCQRLAAASILLARFLGYVGETPNR"
     gene            complement(6850..7848)
                     /locus_tag="VF958_10680"
     CDS             complement(6850..7848)
                     /locus_tag="VF958_10680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9149613.1"
                     /translation="MWVIWRIPLDGGKPVRETSGPGPETHPAVSRDGRLLVYSTASPD
                     RDILVIDRSLGTISRLASARTDVMPAMAPDGSAVVYVSDRLGKDDLWLQAMTGGRPSP
                     TPPKRLTDLETGSGPATPVFSPDGQWLAYFRQSKGQRDIWMLPIKGGVPVQVTNHPAQ
                     EFHPAFSPDGSQLAFVSSRSGPEHVWILPVRGASVSGEPWQLTEGEQFDWFPSFSPKG
                     DFVAFVRGGEIWVTTPKRGASCRQVTTGAEIHYCAWEPDGRSLLASGMWGTSILRMRD
                     VRLESGASEPLTPDVALGSRDAPGYFGLSRDGRYLAVDAVQTKGNLWTVGSRAGRS"
BASE COUNT         1358 a         2541 c         2681 g         1391 t
ORIGIN      
        1 cctcaccacg accgtggagg gaaggaaccg gtttcccgtc cgggtgcggt acagccgtga
       61 actccgagac gatcccgatt cgatccgtcg aattctcgtt cccgcggcgg ggtcgggcgg
      121 gatgttaccg gcctccgcgc cgccggcgga gcgcatggaa gagaccacga cggaaacctc
      181 cggagcccgg gcgccgttcg ctggcgcccc aaaccctctc ggcgcacgcg tgcagatccc
      241 gctcagcgaa gtcgcagaga tccggttccg accggggccg tcaatgatct cgtcggagaa
      301 cgggctcctg cgggaacggg tatttctgaa cgttcggggc cgtgacgtcg gatccctcgt
      361 ggacgaggcg aaacgggtcg tcgagtcgaa ggtcgctctt ccggccggat atttcgtcga
      421 gtgggcgggc caattcgagc accagctccg cgcgcgcaac cggctcatgg tcgtcgtgcc
      481 ggtgtgtctg gcgatcatct ttctcctgct ctatctgacc taccactcga tcaaagaggc
      541 cgcgcacgtc atcctcgcca ttcctttcgc gctgacgggc ggaaacttcc tcctctggtt
      601 gctccaccgc ctcgccgtca ccaacggatg gaagaccgag ttccacatga gcgtcgcggt
      661 ctgggtcggg tacatcgccc tcttcggcac ggcggtgcaa acggccgtcg tcatggtcgt
      721 ctatttggag gaagcactgc agcggaaggc cggcgaggga cctctgacac gttcctcgat
      781 ccgcgaggca gcgatggaag gcgcggtcct gaggctccgg ccgaaactca tgacggtgtc
      841 gaccgttgtt ctcgggctcc tcccgatcat gtggtcgacc gccacggggt cggaggtcat
      901 gaagccgatc gcagcgccgg tgctgggagg gatgctctcc tccctcgccc acgtattgat
      961 cgtcaccccc gtgatctgga cgatgttgaa ggagcgcgag ctgcggaaag gtcggatcga
     1021 ggtcggaaag gtgagcgacc ttctgaaggc ctgagtacgg attcgaatca gatgcccttg
     1081 cggcagtagc agtagacgaa ctgctgcacc ttgcccagcg gcgtgtggtg ttcttccttc
     1141 tcgtggctca cgagcgtaaa ggtctctccg aactcggagt gcagctgctc cgcgccgtag
     1201 cgcaccaccg gcagcccgct gcactccgtc gggccgtctt cggcaaacgt tgcgacgatg
     1261 acgtgaccgc cgggcttcac ggacttcgag accgtgcgga cataagcggc gcgatcttcg
     1321 tccgacgtca ggaaatgaaa aacggcacgg tcgtgccaga tgtcgtactc gttcggaggc
     1381 aggtcgacct tcgtgacgtc ggcctcgagc caccggacgg cggaggcgcg cgcgccgagc
     1441 cttttccttg cggcggcgag cgccgtcccc gacaggtcga ggacgctcaa ccgtgagtag
     1501 cccgcggcga gcaggtcgtc cacgagcgtt gaagctccgc cgccgacgtc gatgatcgcg
     1561 gcgtccttcg gcgccccggt cgcccggatc agccgaagcg agctctcggc gtgctcctgg
     1621 taccagctga ccgcgttcgt cggcctggtg gagtagatcc gctcccagtg ctctttggac
     1681 agcatcgttc ggtcggcctc agtcctcgtg cggcggcgta ccgggcgcat gagtatgctc
     1741 cggtttcgcg gcccctccgg gtggcggtgc ctcctcgtgg tgatgttgcg tttcttccgc
     1801 ctcaggcttg agggcggcag tctcttcttc cgtgatctcg gggaggtgcc gaaggaaagc
     1861 gacggtctgc cagatctcgt tctcgctgtg ggtcggtgag aacgcgggca ttccgctcat
     1921 tcggatcccg ttcgacgtga tccagaagag ctctccgtca ctcctcgcct ggacacgccg
     1981 gagcgtgaga tcgggcgcac ccgggttcag gccctgtccg atctcccggg ggtcgacgcc
     2041 cggcgcgccg tggcagatca cgcagttctc tcggaagtgc gccagacccc cgcggagggt
     2101 ttcggcattg gctgcgagag gattctttcg cgcgggcgcg cgccgcgcca ccgccttgtt
     2161 cagtgcaaac gtcgcaagcg tcctttcggt cttgctcggc ggcacaatcg ccgagacgtt
     2221 gaaggagcct gtgtagacga tggcggccgc tgcgatcgcg accagcagga ttccgaagag
     2281 caccccgaca agaaacctca ctctcgcctc cgttctgcca tctaggcgtg atccggcgcg
     2341 gtgaagcagt cgcgctcggg cccgatctcc acctggatgg tcgcatgttc cacgccggct
     2401 tcgctgcgca ggagctcctg cacgtcgtgc aggacctgtc cccgcgggct atccgccgtc
     2461 gcccgaatgt gaacgctcgc cgagtgagag cccgacgtca gcgtccagaa gtgaaagtcg
     2521 tgggcctctt cgactccggg gagagcgagg atgtgctcgc gaagggacgg caggtcgatc
     2581 tcgcgaggag caccctcgag aaggatgtgc gccgactgcc gcaggaatcc gaccgcgcgc
     2641 gggaggacga agagggcgat caagccggag gcggcgaggt cgaaccagag ccggcccgtc
     2701 agggcgatcg cgagccccgc ggcgaggacc gcgagagacc cggcggcgtc catcacgacc
     2761 tcgaggtagg ccgcgcggac gttgaggccc ccacgccggt cgggggcgag aatcgccatc
     2821 gagacgaggt tggcggcgag tccgaccact gcgacgggga tcatcacgct caaccggatg
     2881 acggaaggat cgtcgagccg gcggaagcct tcgtacagaa gccagacgca gacgagcagg
     2941 aggatctggg cgttgacgaa cgccgcgagg acctccgcgc gcacgagccc gaaggtgtgg
     3001 cggctcgtcg gcgcgcggct cgcgagcgcg gcggccgcgt aagcgagcgt gagcgccgcg
     3061 acgtcggcga gcatgtggtt cgcgtcggcc accagcgcga ggctccccga cacgagtccg
     3121 cccaggactt cgagaaagag caccgagagc gtgacgccga gtgccgcgcg caggcgccgc
     3181 ttgcgagacg tcggctccgg cagcgcggag ttcgcgctcc cggcgtttgc ggctacgctc
     3241 cccacccgac gatcttgtct accatcgcgg ccgcgatcgg gtgataggtc gggcgcgcct
     3301 tgcggtagat ggcgagggcc cgcgccttgc cttccggcgt cttgaccagg gcctcgtaga
     3361 gaggcttcag gaacttccgc ctgcccatcg acgtcaagaa ttcctcgagt cggggatagg
     3421 cgggctcata cccgcttgcg atcgcgatcg agagccactg gttcgtgatc tcggagttgc
     3481 cggaggccgt cagagcgaaa gtgcgatcga gctccgccat ccggtctcga tcgagcggga
     3541 gcggcaaggc gcgcagaaag tgaagccatt cctgggtgct ccaggcgcgc gtgtcgagag
     3601 ccgaggcggg cgccttccgc tcgagccagc gtcctgtcgc ggcctccacc gcgacgaacg
     3661 cctgggagcg cggtcgcggc gctcccgcgg ggagcccggg tctctcgatc cattcttcga
     3721 gcggtatgcg ggcggcgagc tccggactgg cgtcgaaaag gttcgacttc agatagcgga
     3781 cgaagtcctc ggtggtgatg ctctggaagg cgaagtgatc gaagtagctc cggagaaacc
     3841 ggtcgaactg ctgtcggccg aacgtttcct cgagcagacg catcaggagc gcgcccttct
     3901 cgtacgggac gtcggtgaag ccgtcgtccg ggtcgcggcc ggcgaggtcg acgtgaagga
     3961 tctcgtcctt gtccggtaga tccgccattt ccttctcgag gtcctgccgt ccgagcaccg
     4021 cctccatctc cgcccggcgg acgccgtaga cccgctcgat gatgcgccgt tcgatgtacg
     4081 tcgtgatccc ctcgttcaac cagaagtcgc tccaggtcgc attggtgacg aggttcccgg
     4141 cccaggaatg ggcgagctcg tgcgccacaa gagcgacgag actcttgtcg ccggcgagca
     4201 ccgtcggtgt cgcgaacgtg aggcgcgggt tctccatccc tccgaacgga aagctcgggg
     4261 gcaggacgag cacgtcgtac cgctcccacc gatacggtcc gaacagctct tcggtcgccc
     4321 ggatcatctg ctcgaggtcc gagaactcgc gggctgcccg ctcgacgacc gtgggctcgg
     4381 cccagactcc cgtgcgcgga gaaacgggaa gggccgcgag gtcccccact gcgagcgcga
     4441 tcaggtacga cgggatcggc tgagtcatct cgaagtcgta ctcgccatcg cgcacgaccc
     4501 gcgggtcgtt gttcgcactc atcaccgcga ggagattctt cggcgtccgc actctcgcgc
     4561 gatatgtgat ccggacctgc ggcgagtcct gaagcgggat ccagctccgc gcgtggatcg
     4621 cctgagactg cgtgaagagg aaagggttct tccgggcggc ggtctgttgc ggatcgagcc
     4681 actggagtgc cgaggcctgc gggcgcgtcg cgtatgtcac gcgcgcccgg gtcgcgtcct
     4741 cgggaacgac gaccgtaagg gccgagccga gaatcttgtc cgccggagcg agggagaagg
     4801 cggcgtccga ccacactgtc ccatccgtcg atgtttccac tttctcgaca tccagatccc
     4861 tcgtatcgag gacgagacgg gtcgcgcggc cgcccgcggg acgttcgagc aggaggtccg
     4921 ctctcccgcg aagaatcctc tgctcgaaga ggacgtccca gtcgaggttg gcgtggcgga
     4981 cgcggatctc ttcgggattg ccgtacgagt gcccgtcccg ggaggtgcga gtggcggcag
     5041 tcgccgaggg cgtcgcggcg gatgccggtg tagcgctcgg tcccccgcag cccgcagccg
     5101 caagggacat cacgaccaca gcagcgagct gctcatatcg cacgggcgtc ttccttccgc
     5161 gatgtccctc acccccgcgc gattataggt ttgcgtggct gttggtggcc cacccgaggg
     5221 cggtcacgga tcaagaacca gggtttctgg ctcttgtcgt tagtcaagaa ccgtcggcag
     5281 gccgaactgg ctgagtcgat cgcatgcgaa ctttccgtgt ccgatgctcg gcctcggaag
     5341 cctcaggcca gtttgcgcgg gacaccgccc gatgaaaagc catgcgatgc ttctcgatga
     5401 ggcggttgaa gtcgcactgg atcgcgggaa tccgatccgg ttcggccgcg cccgcgctga
     5461 aggcgatcga cgcggggcag atgacgcagt tcaagagggc cgggcattcg ccgcagcgac
     5521 cgaaggggga gtacttcgcc gtcttgccgg tgagaagtgg aaggtctcga accttccgct
     5581 cgcgggccgc gagcttctca gcgaggtcag gatcggtgag gtcgaatgtg ccaagagtct
     5641 cgatggcgtc gcgcaggagc ggcggagggt ctcgcaggca cgatcttgcc atcgccccgc
     5701 acggggcgag agagccgtcc atgtcgacaa agagggactt ccggcctgcg gtcccgcaca
     5761 gagagggggc gtaggcttgc ccggaggttt ctcgcccgtc gggctggaac gcctggaacc
     5821 agacttcccc agaggacgcg tgcaccttca gcgactcctc gacgacatcg gcgagctgcc
     5881 tgtcgagttc gtcccgcgcg tcgaggcccc atccggagtc gtgcgtgaag accgggacga
     5941 ctcggatctc gcgcacgccg cgggagacga aataacgaaa cgaaccggcc agatacgaga
     6001 catttcccga gctgagcgtg accgaaacag cgaacctcgt gcggagatga ccggggtggt
     6061 cgcgcccgaa ccgtacgagg agtcggtcga gaatgtcgaa gctgcccggc ccccgctggt
     6121 tctgggcacc gggcagcccg tcgatggaaa gagtgatgtg aacgtcggct tccaccagaa
     6181 accgagccgt ctcctcgtcg aggaagactc cattcgtaga gatctccaga tcgggcgtca
     6241 tcccggttgg tgtctctgct cgaacgattt cgacagcgcg gcggacaagc gacatctcca
     6301 gcagcggctc gcctccgaaa aacgcgagag ttggatggct gtggtccgac cgaagaaagc
     6361 ggtcgacggc cgcctcgaga acgcagagct gcatggatcg cggtgcgctt cggtcctggt
     6421 aacagtacgc gcaccgaaag ttgcagcgcg tcgtcaacat cagggcaacc aggctcggac
     6481 cggcagcgaa gagatcttcc agcatggctc cgggcgcgtt cagcgatttg gtgtctctcc
     6541 tacgtatccg aggaatcggg cgaggagtat ggacgctgcc gcgagacgct gacagtcccc
     6601 cggcctgggc tcggtgatac acgcccttac gggtttaact aatggaccgc cggcaccgat
     6661 tggcccttcc ctgagttcga ctatgaactc tgtctctgtc tgctttttaa gtacgttcag
     6721 gtaatggatt gtgggttgtg tcagtgcctc tagcagttcc atgcatttga tcgcctcgtc
     6781 gacttcgact ttcatctcgg tgtcctcctt tcaggcgccg gtcaatccga cggccgaaac
     6841 agacgcttct catgagcggc cggcacgcga gccgaccgtc caaagattgc ctttcgtttg
     6901 aacggcgtcg acggcgagat accggccgtc gcggctgagc ccgaaatacc ccggggcatc
     6961 gcggctaccg agggcgacgt cgggtgtcag cggctcgcta gcgcctgatt ccaagcgcac
     7021 gtctcgcatc cggaggatcg aagtgcccca catcccgctc gcaagaagcg agcggccgtc
     7081 gggttcccac gcgcagtaat ggatttccgc gcccgtcgtc acctgtcgac aggatgcacc
     7141 gcgcttcgga gtggtcaccc agatctcgcc accccggacg aatgcgacga agtcaccctt
     7201 cggggagaag gaggggaacc agtcgaactg ctcgccttcc gtgagctgcc agggttcccc
     7261 gctgactgat gccccacgaa ccggaaggat ccaaacgtgt tccggccccg acctgctgga
     7321 gacgaaagcg agttgcgatc cgtccggcga gaaggcggga tggaactctt gagccggatg
     7381 attcgtgact tggaccggta caccgccctt tatcggaagc atccagatgt ccctctggcc
     7441 tttcgactgc cggaagtacg ccagccattg gccgtccggc gagaagacgg gtgttgcggg
     7501 acccgacccc gtctcaaggt cggtgaggcg ctttgggggt gttggagatg gtcggccgcc
     7561 cgtcatggcc tggagccaga gatcgtcctt ccccagccga tcggagacgt agaccaccgc
     7621 gctcccgtcg ggtgccatgg caggcatcac atccgtacgc gcgctggcga ggcgggagat
     7681 ggttcccagg ctccggtcaa tcacaaggat gtcccgatcg ggagaggcgg tcgaatagac
     7741 gagcaggcgc ccgtcacggg agactgccgg atgtgtttcg ggccctggcc ctgacgtttc
     7801 gcgcacgggc tttcccccgt cgaggggaat acgccagatc acccacatcg cgtcgatccc
     7861 gccccgtcgc gcagagcagt agacgaagtt tccggacgcg gaccaggccg gctcgacgta
     7921 tgaaacgtca ccgggcgtga gctgcctcgg ttttcctttt tcgaggtcaa c
//
LOCUS       DASWAV010000510         5878 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U2665
            k141_309253, whole genome shotgun sequence.
ACCESSION   DASWAV010000510 DASWAV010000000
VERSION     DASWAV010000510.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960316
            Sequence Read Archive: SRR10260007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 5878)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5878)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:47:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,929
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5878
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_309253"
                     /isolate="SMAG_U2665"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.36444 N 132.31889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            109..531
                     /locus_tag="VF958_10685"
     CDS             109..531
                     /locus_tag="VF958_10685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9149614.1"
                     /translation="MKVKFYYSSKDKPGGQYPCDNAAALEKIKALKARGADVEAIDVA
                     SVEDVFRFYHAALTGPPATVRAVFGMKGGLEADFGRATPALLIFGDPKDRYPTDAYPR
                     QDQARGGLVGCEKALDDLSKELAKLPTTEDEEDQPVTR"
     gene            528..2195
                     /locus_tag="VF958_10690"
     CDS             528..2195
                     /locus_tag="VF958_10690"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase"
                     /protein_id="HEX9149615.1"
                     /translation="MSTIPRDYLPPKEFQPERIYKLKEFESYPDPLNSTEELLDKQVA
                     AGRGDNRAILIGDKVITYKQVLGASNKIGNALRKLGVEEADRVMLRSPNVPPAIFTNF
                     GALKIGAVMVPTSPMLSPAEITHIANNAEAKVLVVAAAFLEGVLKARPNLKTVKHVIV
                     FGGQPEELKAQGLLCYEEIVANESPALDPVRRPRTAVSVLLYTSGTTGMPKGTAHYME
                     EALIVPDTFAKYGWNVQPDDIICGPAPISLAAGYSTVATIPYRFGAAVSLIPKFTPEG
                     LFEQIQSHKVTILSALPTAYRKMLEVPDAEKKYDLSSLRVLTGGGESLTAKTYLDWKN
                     RFNQEIYEGLGTTEMMYVFVSSVATRKVKPGAIGTAIPGYEIQVITEEGKVAKPGELG
                     RLYARGPTGTLYWRPLEEGGSLLEKQKSLIREGWVLVGDFCTVDEDGYLSFVSRQEDL
                     IKSSGYRIGPEEIEDALLKHPAVADAGVIGVPDPVRGQNVKAFVILRPGHAPSEELKQ
                     QILDSCREHIAIYKLPRIVEFVTELPRTLQGKLLRRVLRDKELVPAS"
     gene            2301..2930
                     /locus_tag="VF958_10695"
     CDS             2301..2930
                     /locus_tag="VF958_10695"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017246682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX9149616.1"
                     /translation="MLDLSSLFGQIDIYLFDQLLRGRIVPGMSVLDAGCGRGRNLVFF
                     LRQGYQVSAVDPDPEAIRAVRILAERLAPDLPLSNFREEAVESASFPDHAADVVISSA
                     VLHFARDESHFRSMLRGTWRLVKPDGVLFCRLASSIGMESRITPRGSRRFGLPDGTER
                     FLVDEPYLLELTSEMGATLVDPLKTTVVQDRRCMTTWVLKKDAGRGGVV"
     gene            2942..3352
                     /locus_tag="VF958_10700"
     CDS             2942..3352
                     /locus_tag="VF958_10700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014630721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase"
                     /protein_id="HEX9149617.1"
                     /translation="MTEPAVDSIASARLTVESSDLASVLNLEPGDIFPPVFATSRMVA
                     LMEVAAARVLGPHLSAGELSVGVTVDIVHTAATLPGATVTATAKFVGKDGKLFLFDVS
                     AADDAGEIGRGTHKRAVVSAERLMAGAARRGARP"
     gene            3364..4161
                     /locus_tag="VF958_10705"
     CDS             3364..4161
                     /locus_tag="VF958_10705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012694293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX9149618.1"
                     /translation="MPKRGVPARIEVLGQHLEYAWIGPQPDEAPTIVFLHEGLGCLAM
                     WRDFPDRLASGTGCGALVYSRMGYGASDPVPGPRSVRFMHQEALEVLPAVIDRFQLQQ
                     VVLFGHSDGASIAVVYAGARLGAVRGLVLEAPHVFVEPVCVESIARIAQGYGTTRLRE
                     KLGRYHGDNTDSMFRTWTDVWLRPEFRTWNIEEYLPSIESPVLVVQGEDDEYGTIRQV
                     EAVVTQVSGLARSLVLARCGHSPHSDQPDAVLEAAGRFVRETLDFER"
     gene            complement(4180..4947)
                     /locus_tag="VF958_10710"
     CDS             complement(4180..4947)
                     /locus_tag="VF958_10710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018352740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase/fumarate reductase
                     iron-sulfur subunit"
                     /protein_id="HEX9149619.1"
                     /translation="MKLTLKIWRQEGPRAPGRLVDYPVDDASPDMSFLEMLDILNIRL
                     LTQGERPIAFDSDCREGICGMCGFLIGGVAHGPQKLTTTCQLFLRHFHDGDTIVVEPF
                     RSRGFSVIKDLVVDRSTFDRIQQAGGFISARTGSAPEANSILIPKEVADRAMDAATCI
                     GCGACVAACPNGSAMLFTAARVSHLSLLPQGKPEREQRVLRMVHEMERLGFGSCSNHG
                     ECEAVCPKGISIDFIARLNREFIRASAVETMEPVRKV"
     gene            complement(4944..>5878)
                     /locus_tag="VF958_10715"
     CDS             complement(4944..>5878)
                     /locus_tag="VF958_10715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018835569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HEX9149620.1"
                     /translation="PRDVASRAAKAVCDEGRGVGPGRRGVYLDFADAFSRLGEDVIRE
                     RYGNLFEMYEQITGENPYRQPMRIYPAPHYAMGGLWVDYNLMSNLPGLHVLGEANFSD
                     HGANRLGASALMQGLADGYFIIPCTIGDYLARVSPKDRLPADHPAFREARKDAEERIG
                     RLLSVNGDRPVESFHRELGDLLWNSCGMARNEKGLKALLERIPALREEFWKRVRVSNE
                     GEGFNQSLEKAGRVADFLEFAELLAYDALQRDESCGAHFREEHETPDGEALRDDARFA
                     YVAAWEFTGVGQPPVLHKESLAFEAVHLTQRSYK"
BASE COUNT         1046 a         1907 c         1900 g         1025 t
ORIGIN      
        1 caatctcctg gaggagcacc gcgtccacat cgccgagtgg ctgggcgacg cctacttcga
       61 aacggaatgg caggtgaact gccccatgga tttctgagag gagaaagcat gaaagtcaag
      121 ttttactatt ccagcaagga caagccgggc gggcagtacc cgtgcgacaa cgcggcagcc
      181 ctggagaaga tcaaggcgct caaggcccgc ggcgccgacg tcgaggcgat cgacgtggca
      241 agtgtcgagg acgtgtttcg cttctaccac gcggccctga cggggcctcc cgcgacagta
      301 cgggccgtct tcgggatgaa gggcgggctc gaggccgact tcggccgcgc gactcccgca
      361 ctgctcatct tcggtgaccc gaaggaccgc tatcccacgg atgcctaccc gcggcaggac
      421 caggcccgcg gggggctcgt cgggtgcgaa aaggctcttg acgacctcag caaggagctc
      481 gccaagctgc cgacgacgga ggacgaagaa gaccagccgg tgacccggtg agcacgatcc
      541 cgcgggacta tctgcccccg aaggagttcc agcctgagcg gatctacaag ctgaaggagt
      601 tcgagagcta tccggatccg ttgaacagca ccgaggagct tctcgacaag caggtggccg
      661 cgggccgggg cgacaaccgc gcgattctga tcggcgacaa ggtcatcacg tacaagcagg
      721 tcctgggggc gtcgaacaag atcggcaacg ccttgcgcaa gctgggcgtc gaggaagcgg
      781 acagggtcat gctccgatcg ccgaacgtac cgcccgcaat cttcaccaat ttcggtgctt
      841 tgaagatcgg cgccgtcatg gtcccgacct cgccgatgct ctctccagcc gagatcaccc
      901 acatcgccaa caacgccgaa gccaaggttc tggtcgtggc ggcggcgttc ctggagggcg
      961 ttctcaaagc gcggccgaat ctgaagacgg tcaagcacgt gatcgtattc ggggggcagc
     1021 cggaggagct gaaagcgcag ggactcctct gttacgagga gatcgttgcg aacgaatcgc
     1081 cggccctcga tccggtccgc cggccgcgga cggccgtgtc cgtcctgctc tatacctcgg
     1141 gaacgacggg aatgccgaaa ggaaccgccc actacatgga agaggccttg atcgtcccgg
     1201 acaccttcgc caagtatggg tggaacgtcc aacccgatga catcatctgc ggtccggccc
     1261 ccatctcgct tgccgcaggt tactcgactg tggcgacgat cccctacagg ttcggcgccg
     1321 cggtttctct gatccccaag ttcacgcctg aaggcctctt cgaacagatc cagagccata
     1381 aggtgacgat cttgagcgct ctgcccacgg cgtaccgcaa gatgctcgag gtcccggacg
     1441 cggagaagaa gtacgacctg tcgagcctgc gcgtactgac gggaggcggc gaatcactga
     1501 ccgcaaagac ctatctggac tggaagaatc gattcaacca ggaaatctac gagggcctcg
     1561 gcacgaccga gatgatgtac gtcttcgtct cgagcgtggc cacgcggaag gtgaagcccg
     1621 gagccatcgg cactgccatc ccggggtacg agatccaggt catcacggag gaaggcaagg
     1681 ttgccaagcc cggagagctg ggccggctgt atgcgcgcgg ccccacggga accctctact
     1741 ggcgtccgct cgaggaggga ggaagcttgc tcgagaagca gaagtccctg atccgcgaag
     1801 ggtgggttct ggtcggcgac ttctgcacgg tcgacgagga cggatacctg agcttcgttt
     1861 cgcgccagga ggacctgatc aagagctcgg gttacaggat cggtcccgag gagatcgaag
     1921 acgctctctt gaagcatccg gccgtggccg acgccggcgt gatcggggtt cccgaccccg
     1981 tccgcgggca gaacgtcaag gccttcgtca tcctgagacc gggtcacgca cccagcgagg
     2041 agctgaagca acagatcctc gacagctgtc gggaacacat cgcgatctac aagctgccac
     2101 ggatcgtcga gttcgtcacg gaacttccgc gcacgctgca aggaaaactc ctgcggcggg
     2161 tcttgcggga caaggagctc gtccccgcga gctaggagtg tccgggtaat ctctcgcggg
     2221 ggaccttcgg gttccccgcg ccgtccctga ggcggggccc aatggtcgat ccggtatcgt
     2281 cgagcagcgt caatcgcgcc atgctggacc tttcgtccct cttcggacag atcgacatct
     2341 accttttcga tcagcttctc cgaggccgaa tcgtcccggg gatgagcgtg ctcgacgccg
     2401 gctgcggccg tgggcgtaac ctcgtcttct tcctgcgcca gggctaccag gtctccgcgg
     2461 tcgatcccga tccggaggcg atccgcgccg tccggatcct cgccgagcgg ctcgcgccgg
     2521 accttcctct ctcgaacttc cgagaggagg cggtggagtc cgcgagcttt ccggatcacg
     2581 cggccgacgt cgtcatcagc agcgccgttc tccatttcgc ccgagacgag tctcacttcc
     2641 ggtcgatgct ccgaggcacc tggcgcctcg tcaagcccga cggtgtcctc ttctgccgcc
     2701 tcgcgtcctc gatcggcatg gaaagccgca tcactccgcg gggctcgcgg cgattcggcc
     2761 tgcccgacgg caccgagcga tttctcgtcg acgaaccgta tcttctcgaa ctcacgtcgg
     2821 aaatgggggc gactctcgtc gatcctttga agaccaccgt agttcaggat cggcggtgca
     2881 tgacgacgtg ggtgctgaag aaagacgccg gacggggagg cgtcgtatag tttggaccgt
     2941 tatgaccgaa cccgccgtcg attcgattgc ttctgcgcgt ctcaccgtag agtcctccga
     3001 cctcgccagc gtgttgaatc tcgagccggg agacatcttt ccccccgttt tcgcaacgtc
     3061 ccgcatggtg gctctcatgg aggtcgcggc cgcgcgcgtt ctcggcccgc atctcagtgc
     3121 cggcgagctt tccgtcggcg tcaccgtcga catcgtccac acggccgcca cgctgcccgg
     3181 cgccaccgtc accgccacag cgaagttcgt cggcaaggat ggaaagctgt tcctcttcga
     3241 tgtctccgcc gccgacgacg cgggcgagat cggtcgggga acacacaagc gggcggtcgt
     3301 gtccgcggaa cggctcatgg ccggcgcggc ccgtcgggga gcgcgtccct gaagagataa
     3361 acgatgccga agcggggtgt cccggcgagg attgaggtcc tcggacagca cctcgaatac
     3421 gcctggatcg ggccgcaacc cgacgaagcc ccgacgatcg tgttcctgca cgaagggctg
     3481 gggtgcctgg ccatgtggcg cgactttccg gatcgcctgg cgtcggggac cggctgcggc
     3541 gcgctcgttt acagtcggat gggttatggc gcgtccgacc cggtcccggg gccgcgatcc
     3601 gttcgcttca tgcaccagga agcgctcgag gtgctccccg ccgtcatcga ccgtttccag
     3661 ctccagcagg tcgtcctctt cggccacagc gacggcgctt cgatcgcggt cgtttacgcc
     3721 ggcgctcggc tgggagccgt tcgggggctg gttctcgaag cgccgcacgt cttcgtcgag
     3781 cccgtctgcg tcgaaagcat tgcgcggatc gcgcaggggt acgggacgac ccgtctgcga
     3841 gagaagctcg ggcgctatca cggcgacaac accgactcga tgttccgaac gtggaccgat
     3901 gtgtggctcc ggccggagtt tcggacctgg aacatcgagg agtacctgcc ttcgatcgaa
     3961 agcccggtgc tggtcgtgca gggcgaagac gacgagtacg gcacgatccg gcaggtcgaa
     4021 gccgtggtca cgcaggtcag tggtctggcc cgatcgctgg tgctcgcgcg ttgcggccac
     4081 tccccgcact ccgatcagcc ggacgcggtg ctcgaggccg ctggtcgatt cgtccgagag
     4141 acactcgact tcgaacggtg aggggacagg aacctttttc tagaccttgc gcaccggctc
     4201 catcgtttcg acggcgctcg cgcggatgaa ctcccgattg agcctcgcga tgaaatcgat
     4261 actgatgccc ttgggacaga cggcttcgca ctctccgtga ttcgagcagc tcccgaagcc
     4321 caaccgctcc atctcgtgga ccatccgcag gacgcgctgc tctctctcgg gtttgccctg
     4381 ggggagaagg ctcaagtggg agacccgggc cgcggtgaac agcatcgcgg agccgttcgg
     4441 gcacgctgcc acgcacgccc cgcacccgat gcaggtcgcg gcgtccatgg cgcggtccgc
     4501 aacctctttg gggatcagga tggaattggc ttcgggtgcg ctgccggtgc gcgccgagat
     4561 gaagcctccg gcctgctgga tccgatcgaa ggtgctccga tccacgacga ggtccttgat
     4621 cacggagaag ccgcgcgagc ggaaaggctc cacgacgatg gtgtctccgt cgtggaaatg
     4681 gcgcagaaag agctggcacg tcgtcgtcag cttctgcggc ccatgggcca cgcctccgat
     4741 caggaagccg cacatcccgc agatgccctc gcgacaatcg ctgtcgaatg cgatcgggcg
     4801 ctctccctgc gtcaggagcc ggatgttcag gatgtcgagc atctcgagaa acgacatgtc
     4861 cggactcgcg tcgtcgacgg gataatcgac cagccgcccc ggcgctcgag gaccttcctg
     4921 ccgccagatc ttcaaggtga gcttcatttg tagctccgct gggtcaggtg cacggcctcg
     4981 aacgcgagcg actccttgtg caggaccgga ggctgtccga ccccggtgaa ctcccaggcc
     5041 gcgacgtacg cgaaccgcgc gtcgtcccga agcgcttcgc cgtcgggcgt ctcgtgctcc
     5101 tcgcgaaagt gcgcgccgca cgactcgtcg cgctggagag catcgtaggc gagcagttcg
     5161 gcgaattcga gaaaatcggc cacgcggccc gccttctcga gcgactggtt gaagccttcg
     5221 ccttcgttcg acaccctcac tctcttccag aattcctcgc ggagcgccgg gattctttcg
     5281 agcaaggcct tcaacccctt ctcgttgcgc gccatgccgc aggagttcca gagaaggtcg
     5341 ccgagctcgc ggtgaaaaga ctcgaccggg cggtccccgt tcaccgagag gagcctgccg
     5401 atccgctcct cggcatcctt ccgggcctct cgaaacgcgg ggtggtccgc cggcagccgg
     5461 tctttcggcg agacgcgggc gagatagtca ccgatggtgc aggggatgat gaagtatccg
     5521 tcggccagac cctgcatgag cgcgctcgcc ccgagccggt tcgccccgtg gtcggagaag
     5581 ttcgcttcgc cgaggacgtg caggcccggc aggttgctca tgaggttgta gtcgacccac
     5641 aggcctccca tcgcgtagtg gggagccggg tagatgcgca tcggctgccg ataggggttc
     5701 tctcccgtga tctgttcgta catctcgaag aggttcccgt aacgctcccg gatgacgtcc
     5761 tcgcccaggc gcgagaaagc gtcggcaaag tcgaggtaga cgccacgcct gcccggcccg
     5821 acgccccgcc cctcgtcgca aaccgccttg gctgcccgtg aggcaacgtc gcgaggca
//
LOCUS       DASWAV010000511         4260 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U2665
            k141_228955, whole genome shotgun sequence.
ACCESSION   DASWAV010000511 DASWAV010000000
VERSION     DASWAV010000511.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960316
            Sequence Read Archive: SRR10260007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 4260)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4260)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:47:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,929
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4260
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_228955"
                     /isolate="SMAG_U2665"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.36444 N 132.31889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1080)
                     /locus_tag="VF958_10720"
     CDS             complement(<1..1080)
                     /locus_tag="VF958_10720"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEX9149621.1"
                     /translation="MTLSAGSRLGPYEIVAPLGAGGMGEVYRARDTKLDRDVAIKVLP
                     EKLAEDAAALARFEREAKAVAALSHPNILGIYDLGRDDGVAFAAMELLRGETLRERLE
                     EGALPQRKALEFGLQIAHGLAAAHEKGIVHRDLKPENIFVTVDGRVKILDFGLAKVGT
                     AQPEETKSPTVAATEPGTVMGTVGYMSPEQVRGKPADHRTDIFSFGAVLYEMLCGERA
                     FRADSAAETMAAIAQKDPPLLTESPGGRFAPSVERILRHCLEKRPEERFDTAHDLAFA
                     LETAMGGSSSATQVAPAIVARKPWLRQAVGALALLAALGAGLVLAGRLRAPTKPTFRQ
                     ITYHRGFIQSARFAPDGQTVVYGSTR"
     gene            complement(1077..3731)
                     /locus_tag="VF958_10725"
     CDS             complement(1077..3731)
                     /locus_tag="VF958_10725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011685392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HEX9149622.1"
                     /translation="MTIAAGSRLGPYEILSPLGAGGMGEVYRARDTRLERTVAIKVLP
                     SHLSVSAESRQRFEREAKTISQLSHPHICALHDVGREGDIEYLVMEYLEGETLADRLL
                     KGPLPREQTLRYGVQIADALDKAHRQGIVHRDLKPGNVMLTKSGVKLLDFGLAKAMAP
                     ATPQSSLTALPTQHALTQEGTILGTFQYMAPEQLEGREADARTDIFAFGCVLYEMATG
                     KKAFSGATQASLISSILRDDPQPISQVQPMSPAALDRVVQKSLAKDPEDRWQNAADLG
                     SELKWILEGGSQAGVPAPVVSRGRRRTWLLSAAAIPVAALLVLLGYQLRPVRPGPVVR
                     SSLGLPAGLQFDGENNAVALSRDGRRLAFAAFETGKRPQLWVRATNSLTVQPIAGTDG
                     ASQPFFSWDGESIGFFADRKLKRVPVAGGTVQAICDAPDPRGGSWGRDGVIVFAPTPY
                     GGLSRVSASGGTPSPATRPGDSGITDRMPSFLPDGRRLLFFRGVGIRTTTKGVGIWSL
                     DLATGKTALVAQEHSGAVWVEPGFLVFVRNATLMAQRADEKSLRTVGEPVPLADGIWF
                     NAYRWTGNYSLSDTGLLVFEGGGLAGKSQLTWMDLDGKIVGTVGEPAPNMGLALSPDG
                     RRALTSVPEPNGFRSIWMYDLTRGVASRFPADGPFADSPVWSPDGTQVVYGDGDGNLF
                     AKASDGLSRARALIRVESANQIATSWYPDGSAIAFAHQDTKTGWDIWILPLSGDRKPY
                     PLMVTSANEQSGRFSPDGKWLSYVSDESGRQELYVVPFPGPGEKRQVTSGGVDDAFWL
                     GTSGELAYALGGKLFAVRVDRQGIGDPRLLLGGRALRPPAGVASWQPSLTTSTRDGKR
                     LLVALPIGEDVSPAMTLVTNWPAELEKR"
     gene            complement(3728..>4260)
                     /locus_tag="VF958_10730"
     CDS             complement(3728..>4260)
                     /locus_tag="VF958_10730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9149623.1"
                     /translation="GRHLLMSVSEGSNYIRVLTAMSEGADSRRLSGESRFSEEHPAIS
                     PDGGWTAFDSSESGQREVYVQPLPDGPKRQVSIGGGQMPVWNRNGSELFYAARDGMLM
                     SVALRVAGGRLEIAEPQPLFLLRLGTSGEIVFQRHSYDVSPDGHRFLVIRRAPDSETD
                     GAVVVTNWTALLKRPR"
BASE COUNT          716 a         1447 c         1390 g          707 t
ORIGIN      
        1 ccgcgtcgat ccgtagacga cggtctgccc gtccggcgca aatcgcgccg attggatgaa
       61 gccgcggtgg taggtgatct ggcggaaggt cggcttcgtc ggcgcgcgga gacgacccgc
      121 gagcacgagg ccggcaccga gagcggcgag cagagcgagc gccccgacgg cctgccggag
      181 ccacggcttt cttgcgacga tcgccggtgc gacctgggtc gctgagctcg aaccgcccat
      241 tgcagtttcg agtgcgaacg cgagatcgtg cgcggtgtcg aagcgctcct cgggacgctt
      301 ctcgaggcag tggcggagga tccgctcgac ggagggggcg aaccgtccgc cgggagattc
      361 ggtgaggagt ggcggatctt tctgtgcgat tgccgccatc gtttcggccg ccgagtcggc
      421 tcggaacgcg cgctcgccgc acagcatctc gtagaggacc gcgccgaacg aaaagatgtc
      481 ggtgcgatgg tctgctggct tgccgcggac ctgctcggga gacatgtagc ccaccgtgcc
      541 catgaccgtt cccggctcgg tcgcggcgac cgtcggcgac ttcgtttcct ccggctgcgc
      601 cgtcccgact ttcgcgaggc cgaagtcgag gatcttcacg cggccgtcga ccgtcacgaa
      661 gatgttttcg ggcttcaggt cacggtggac gattcccttt tcgtgcgcgg ccgcgaggcc
      721 gtgtgcgatc tgcaacccga actcgagggc ttttctttgc ggaagagcgc cctcctcgag
      781 acgttcccgc agtgtctccc cgcggagaag ctccatcgcg gcaaacgcga cgccgtcgtc
      841 gcgaccgagg tcgtagatgc cgaggatgtt cggatgggag agagccgcga ctgctttggc
      901 ttcgcgctcg aagcgcgcga gggcagcggc gtcttcggcg agcttctccg gcaagacctt
      961 gatcgctacg tcccggtcga gcttcgtgtc cctcgcccga tagacctctc ccatgccgcc
     1021 cgcgccgagc ggcgcgacga tctcgtaggg gccgaggcgt gatccggcgg acagcgtcat
     1081 ctcttctcga gctcagccgg ccagttggtc acgagggtca tcgccggaga gacgtcctct
     1141 ccgatcggca gcgcgaccag caggcgcttt ccgtcccgag tcgatgtcgt cagcgacggt
     1201 tgccaggaag cgactccggc gggcggtcgc agcgcacggc cgccaagaag gagacgcgga
     1261 tcgccgatcc cctgccgatc gactcgaacc gcgaagagtt tgcctcccag cgcgtaggcg
     1321 agctcgccgc tcgtcccgag ccagaaggcg tcatcgactc caccggaagt gacctgcctc
     1381 ttttctcccg gtcccggaaa cggcacgacg tagagctcct gccggcccga ttcatcggag
     1441 acgtacgaca gccacttccc gtcgggagag aaccttcccg actgctcgtt cgcggaggtc
     1501 accatcagtg gatagggctt ccgatccccg gaaagaggca ggatccagat atcccaccct
     1561 gtcttggtgt cctgatgagc gaaggcgatc gccgatccgt cggggtacca gctcgtcgcg
     1621 atctggttgg cggactccac gcggatcagc gctcgcgctc tggacaggcc atcggatgcc
     1681 tttgcgaaga gattgccgtc tccgtcgccg tacacgacct gggtgccgtc cggagaccag
     1741 accggagaat ccgcgaacgg gccgtcggcg gggaagcggc tggcgactcc gcgcgtgagg
     1801 tcatacatcc agatgctccg aaagccgttc ggttcgggga cggaggtgag cgcccggcgg
     1861 ccatcgggag acaaggccaa tcccatgttc ggagccggct cgccgaccgt gcccacgatc
     1921 tttccgtcga gatccatcca cgtcagctga cttttcccgg cgagaccgcc tccctcgaaa
     1981 accagcagtc ccgtgtcgga cagcgagtag ttgccggtcc agcggtacgc gttgaaccag
     2041 atcccgtcgg caagaggcac cggctcaccg accgtgcgaa ggcttttctc gtcggcccgc
     2101 tgcgccatca gggttgcgtt ccgcacgaac acgaggaagc ccggctcgac ccacactgct
     2161 ccactgtgct cctgtgccac gagagcggtc ttcccggtcg ccaggtcgag actccagatc
     2221 ccgacgccct tcgtggtcgt gcgaatgccg actccccgga aaaacaggag ccgccgcccg
     2281 tccgggagga agctcggcat gcggtcggtg atgccgctgt ccccgggtcg ggtggccggc
     2341 gacggagtgc ctccggaggc cgaaacccgc gagagacctc cgtagggcgt cggcgcgaag
     2401 acgatgacac cgtcgcggcc ccagctcccg ccccgaggat cgggcgcgtc gcaaatcgcc
     2461 tgcaccgtgc ccccggcgac cggcacgcgt ttcagcttgc gatccgcgaa gaatccgatc
     2521 gattcgccgt cccaggagaa aaagggctgc gatgcgccgt ccgtcccggc gatcggctga
     2581 acggtcagac tgttcgtcgc gcgaacccaa agttgaggtc tcttaccggt ttcgaaggcg
     2641 gcaaaggcga gccggcgccc gtcgcgcgaa agggcgacgg cgttgttttc gccgtcgaac
     2701 tgcaagcccg cgggaagtcc gagactggag cgcacgaccg gtccgggccg cacgggacgg
     2761 agctgatagc ccaataagac gaggagcgcg gcaacgggaa ttgcggccgc gctgagaagc
     2821 caggttctcc gccgaccccg cgacacgacc ggcgcgggga cgccggcctg cgaaccgcct
     2881 tccaagatcc acttcagctc gcttccgaga tcggcggcgt tctgccagcg gtcctcgggg
     2941 tccttcgcga ggctcttctg cacgacccgg tcgagcgccg cgggcgacat cggctgaacc
     3001 tgcgagatcg gctggggatc gtcgcggaga atcgacgaga tcaacgacgc ctgtgtcgcc
     3061 cccgaaaacg ccttcttgcc cgtcgccatc tcgtacagca cgcagccaaa cgcgaagatg
     3121 tccgtccgtg cgtccgcctc tctgccctcc agctgctcgg gagccatgta ctggaatgtc
     3181 cccaggatcg tgccctcctg cgtcagcgcg tgctgcgtcg gaagcgccgt cagactcgat
     3241 tgcggagtcg caggcgccat tgccttggca aggccgaagt ccaggagctt cacccccgac
     3301 ttcgtcagca tcacgttgcc cggcttcagg tcccgatgca caatcccctg ccggtgcgcc
     3361 ttgtccagcg cgtcggcaat ctgcaccccg taacgcagcg tctgctcccg gggaagagga
     3421 cctttcagca accggtccgc cagcgtctcg ccttccaggt actccatgac caggtactcg
     3481 atgtcccctt cccgacccac gtcgtgcagc gcgcagatgt gcgggtgcga aagctgcgag
     3541 atcgtcttcg cctcccgctc gaaacgctgg cgcgattccg ccgaaacgga aagatgggat
     3601 ggcagaacct tgatcgccac cgtgcgctcg agccgcgtgt cccgcgcccg gtagacctcc
     3661 cccatcccac cggcgccgag cggcgagagg atttcgtaag gaccgaggcg actgcccgcc
     3721 gcgatggtca ccggggtctc ttgagcagag cggtccagtt cgtgacgacg accgcgccgt
     3781 cggtttcgct gtccggcgcg cgacggatca cgagaaaccg atggccgtcc ggagacacgt
     3841 cgtacgagtg gcgctggaac acgatctctc cgctcgttcc gagccgaagg agaaagaggg
     3901 gctgcggctc tgcgatctcc agacggcccc cggcaacacg caaggcgacc gacatgagca
     3961 ttccgtctcg agcggcatag aagagctccg agccgtttcg attccagacc ggcatctggc
     4021 cgcctccgat cgagacctgt cgcttcggac cgtcgggcaa gggctgcacg tagacttccc
     4081 tctgtcccga ctcgctcgag tcgaaggcgg tccagccgcc atccggtgag atcgcgggat
     4141 gctcctcgga gaatcgggac tcgccgctca accgtcgcga atcggcccct tcggacattg
     4201 cggtcagaac ccggatgtag ttgctgccct cggagacgct catgagcaga tgccggccgt
//
LOCUS       DASWAV010000512         1507 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U2665
            k141_150623, whole genome shotgun sequence.
ACCESSION   DASWAV010000512 DASWAV010000000
VERSION     DASWAV010000512.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960316
            Sequence Read Archive: SRR10260007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 1507)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1507)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:47:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,929
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1507
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_150623"
                     /isolate="SMAG_U2665"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.36444 N 132.31889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(314..>1507)
                     /locus_tag="VF958_10735"
     CDS             complement(314..>1507)
                     /locus_tag="VF958_10735"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HEX9149624.1"
                     /translation="IGEARLLALATGGSLAALFVFTRGSFRDALGPFASSLATLADLA
                     LLFCALSLTGGFRSPYGLLLPAGVALAWHLEGRSAARFYAAVSLLGVAALVTLGGGRL
                     PPPAELFPLIATGLAAPALLAALEAMGSVPSVSRAVGATGEEPARSAGVASELPPAER
                     HGSDEILHDLKSPLSVARVYTDLIAEGARRGALPNAEHLANLSREIDLAERMVGGTSA
                     VPRPAVSPGEMRFESTPATPPHAAAPQASADLVEILGSLATAYRLSNVGRLRIEFIAE
                     RPELPVTADPVALQRAFRNVLENAIKYTPEGGEVRIRAGAAGPHAFVVVKDTGMGMSD
                     EERARAFDFAFRGAGAVASGARGKGLGLALTKEILEANGGKISLSSEAGFGSEVTILL
                     PTHKR"
BASE COUNT          250 a          523 c          519 g          215 t
ORIGIN      
        1 ggggtcgagc ggagtgtcca cctcgaagcc ttccggcgcg agcaggtcgc gcaccgccac
       61 gccgaccgac ggctcgtcgt cgacgacgac gacccttttc acgtcgcgct cccttgctcg
      121 ggatctggct ggcgccaccg gcgctcggct cgccataacc ggctcgaccg agcgcctgat
      181 ccctcgctgc ggtcgcgctt ccgcggcgcg aggctcctcc ctcgattacc tcgggagact
      241 tctcgcgccg cgacccggag actcttcgcg atgcgagcgg gcgactgatt acggcgcgaa
      301 agagacactt ctctcatcgc ttatgcgtcg gcagcaggat cgtcacctcc gaaccgaagc
      361 ccgcctcgga agaaaggctg atctttcctc cattcgcttc gaggatctcc ttcgtcaggg
      421 cgagaccgag ccccttgccg cgcgctcccg acgcaaccgc cccggcgccc cggaacgcga
      481 agtcgaacgc ccgggcgcgc tcctcgtcgc tcatacccat gccggtgtct ttcacgacga
      541 cgaacgcgtg cggaccggcg gcgccggcgc gaatccggac ctctccgcct tccggtgtgt
      601 acttgatggc gttctcgagc acgttgcgga atgcccgctg cagagctacc gggtctgcag
      661 tgaccgggag ctccgggcgc tcggcgatga actcgatccg caaccgcccc acgttcgaca
      721 accggtaggc ggtggcgagc gagccgagta tctcgacgag atcggcgctc gcctgaggag
      781 cggcagcgtg cgggggcgtc gcgggcgtac tctcgaaacg catctcgccg ggagaaaccg
      841 ccgggcgcgg cactgcggac gtgccgccga ccattcgctc ggccaggtcg atctctctcg
      901 aaagattcgc caggtgttcg gcgttcggca gcgcgccgcg tcgggctccc tccgcgatca
      961 ggtcggtata gaccctcgcg accgacagcg gcgatttcag atcgtggaga atttcgtcgc
     1021 tcccgtggcg ttcggccggc gggagctcgg aggcgacacc agccgatcga gccggctcct
     1081 cgccggtggc gccgaccgcc cgcgacacgg atgggacgct ccccatcgct tcgagggcgg
     1141 cgaggagggc cggggcggcg agaccggtcg cgatgagcgg aaagagctcg gccggcggcg
     1201 gcaggcgtcc tccgccgagg gtcacgagcg cggcgactcc gaggagagag accgcagcgt
     1261 agaagcgcgc ggcgcttcgt ccttcgagat gccacgccag ggcgaccccg gccggcagga
     1321 gaagtccgta aggactccgg aaaccaccgg tgagagaaag tgcacaaaag agaagtgcga
     1381 gatccgccag cgtcgcgagg gaagacgcga aaggaccgag cgcgtcgcga aagctgccgc
     1441 gcgtgaacac gaagagtgca gcgagcgagc ctccggtcgc gagcgcgagc agccgggcct
     1501 cgccgat
//
LOCUS       DASWAV010000513         3576 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U2665
            k141_585968, whole genome shotgun sequence.
ACCESSION   DASWAV010000513 DASWAV010000000
VERSION     DASWAV010000513.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960316
            Sequence Read Archive: SRR10260007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 3576)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3576)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:47:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,929
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3576
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_585968"
                     /isolate="SMAG_U2665"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.36444 N 132.31889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..320
                     /locus_tag="VF958_10740"
     CDS             <1..320
                     /locus_tag="VF958_10740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9149625.1"
                     /translation="PIPVVIEKSRGSSKVLVERSSQIVVSVPTELPTPLPAAFPSDAA
                     PPLRRRIVIEVRPTPTPTVPELELSAPPPPAETPEPPEPEPEETPEAVPTAGPRANAA
                     LAR"
     gene            complement(373..816)
                     /locus_tag="VF958_10745"
     CDS             complement(373..816)
                     /locus_tag="VF958_10745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9149626.1"
                     /translation="MGCQDWTLLAIAGTHGLLPVQLQRTLYLLGQRFPKKLAAGDFYE
                     FRPISSGDYSEQIYRDAEALSQKGLVAIGSSERDGRREYRVTPAGAEKARALAKHVRP
                     AVLRQLHTLAAWARTRSVEQLGRGSSSTAPVVPSDPSRRDPARPR"
     gene            complement(949..1224)
                     /locus_tag="VF958_10750"
     CDS             complement(949..1224)
                     /locus_tag="VF958_10750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9149627.1"
                     /translation="MSTEVAAPLECTAEPPHRFRIDGRIRVRNGVLLTLQRVDERYPP
                     RDMFITSRELKMGYENVFKGCFVCVDRGLSENAIAIPKDHIQAGLPA"
     gene            complement(1214..1579)
                     /locus_tag="VF958_10755"
     CDS             complement(1214..1579)
                     /locus_tag="VF958_10755"
                     /inference="COORDINATES: protein motif:HMM:NF013868.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HEX9149628.1"
                     /translation="MYVEDGEPGGPIVLRLGAMVKVGETVREFESSLERIVSERTGPL
                     VLDLSDLEYMDSTTLGVLVGALHRLKSENREMVLVNPRERIASLLRVAKLDSLFSIYE
                     SVPEAIAAITRREEDTGVN"
     gene            complement(1614..2018)
                     /locus_tag="VF958_10760"
     CDS             complement(1614..2018)
                     /locus_tag="VF958_10760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9149629.1"
                     /translation="MTKPELPHAYPFRFVDTIVRAAGPDFDRGRVSARISAGARAAMG
                     GEWSSPTLLAELIAQAALLLQGGDPDLGRRGFLAGIENFEVHRMPQAGESLTVDVRIA
                     RRFGVIVKFEGEVRSGSERVAGGAILVRQGTP"
     gene            complement(2018..2278)
                     /locus_tag="VF958_10765"
     CDS             complement(2018..2278)
                     /locus_tag="VF958_10765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012646991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantetheine-binding protein"
                     /protein_id="HEX9149630.1"
                     /translation="MANADLAPRVKDLIVRRLKLDIDPASIESDAPLFGEGLGLDSID
                     ALELVLGLEQEFGIKVEDEEVGVKAFASVNALVDFIEQKKIA"
     gene            complement(2321..3049)
                     /gene="fabG"
                     /locus_tag="VF958_10770"
     CDS             complement(2321..3049)
                     /gene="fabG"
                     /locus_tag="VF958_10770"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003181704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase FabG"
                     /protein_id="HEX9149631.1"
                     /translation="MRTALVTGGSRGIGRAIALELASTGHRIVLSYRQDVARADATVA
                     AIRALGGDAVAIASDVTKAADVRALGDRMTSEFGGADVLVNNAGVIKDAIFPLMKEED
                     WDLVLDTDLKGAFRVTKAVVRGMLHKGWGRIINVVSVSGISGHVGQANYSAAKGGLIA
                     FTKTLALELATRGITANAVAPGLVSTEIIAHLKEDARQEFLRRIPMARFGAPEEIAAV
                     VGFLASDRASYVTGQVWRVDGGMA"
     gene            complement(3046..>3576)
                     /locus_tag="VF958_10775"
     CDS             complement(3046..>3576)
                     /locus_tag="VF958_10775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9149632.1"
                     /translation="TRRETVEEVCADLNARGLPVWIGNVNASTQFVLTGGSEAVEEAL
                     RVLAPKSLSVLPLTMSWPIHSPLMEPVAQAVAPVVEACRSIRDPAVPYYGPDGRRART
                     GARVRELLGTEFVHPTLWNDTYEAMVSDGIRMFVEVGPGDMLSKMARWIDRTTTCRPA
                     GSLAAIRTVSDTLGRP"
BASE COUNT          599 a         1245 c         1161 g          571 t
ORIGIN      
        1 ctccgatccc ggtcgtgatc gaaaagagcc ggggctcgtc gaaggtcctg gtcgagcgct
       61 cctcccagat cgtcgtttcc gtcccgaccg agctgccgac gccgctcccg gcggcgttcc
      121 cctccgacgc ggcgcccccg ctgcgacgcc gaatcgtcat cgaggttcgt ccgaccccaa
      181 cgccgacggt gccggagctc gagctcagcg caccaccgcc gcccgccgag actcccgagc
      241 ctccggagcc cgagccggaa gagacgccgg aggcggtgcc cacggccggt ccgcgagcca
      301 acgcagcgct ggcgagatga caacgccgga agagtttgcg aaggcccggc cgtcgtcagc
      361 agcgcgctga agtcagcgag gacgcgccgg atcgcgacgg gacggatcgg agggcacgac
      421 cggagcggtt gagctcgaac cgcgaccgag ctgctccacc gatcgagtcc gcgcccaggc
      481 ggcaagggtg tgaagctggc ggaggaccgc cggacggacg tgcttcgcca gtgcccgggc
      541 tttctcggcc ccggcgggcg tcacgcggta ctcccgcctg ccgtcgcgtt ccgaggatcc
      601 gatggccacg aggcctttct gcgagagcgc ctccgcgtcc cggtaaatct gctcggaata
      661 atcaccggag ctgatgggcc gaaattcgta gaagtcgccc gcggccaatt tcttcggaaa
      721 gcgctggccc agcaggtaga gggttctctg gagctgaacc ggaagaagcc catgcgtgcc
      781 ggcaatcgcc agaagagtcc agtcctggca acccacgcca gcctttcgag caccagttcc
      841 acggaagaaa tccaagggga ggagttccga tcccacttgt ttcacatcgg gagcccggag
      901 tctataggac caaagtctga gacgccgagg gtctgacccg ggaggacgct acgccggcag
      961 gcccgcctgg atgtggtcct tggggatcgc gatcgcgttt tcgctcagcc cgcgatcgac
     1021 gcagacgaag catcccttga acacgttctc gtatcccatc ttgagctcgc ggctcgtgat
     1081 gaacatgtcg cggggcggat agcgctcgtc gacgcgctgg agcgtcagga gcacgccgtt
     1141 gcggacccgg atcctcccgt cgatcctgaa gcggtgcggc ggttccgcgg tgcattcgag
     1201 gggcgctgcc acctcagttg acacccgtgt cttcctcccg tcgcgtgatc gcggcaatcg
     1261 cctccgggac gctttcgtag atcgagaaga gagagtcgag cttcgcgaca cgcagaagag
     1321 aggcaatgcg ttctctcgga ttcaccagga ccatttcgcg gttttcggac ttcaggcggt
     1381 gaagcgcgcc gacgaggacc ccgagggtcg tcgagtccat gtactcgagg tcgctcagat
     1441 cgaggaccaa cggtcccgtc cgttcgctga cgatccgttc caggctgctc tcgaactcgc
     1501 ggacggtctc cccgactttc accatcgccc cgaggcgcag aacgatgggg ccgcccggct
     1561 cgccgtcctc gacgtacacc cctatctccg acgccgcgaa gcgatgcggc tcctcatgga
     1621 gttccctggc gaacgaggat ggcgccgccg gccacgcgct cggatccgga ccggacctcc
     1681 ccctcgaact tcacgatgac gccgaagcgc ctcgcgatcc ggacgtcgac cgtcagactc
     1741 tcccccgcct gcggcatccg atgaacctcg aagttctcga tgccggcgag aaagccgcgg
     1801 cgcccgaggt ccggatcccc gccctgcagg aggagcgcgg cctgcgcgat cagttcggcg
     1861 agcagggtgg ggctcgacca ctccccgccc atcgccgcgc gggcaccggc gctgatccgt
     1921 gccgacacgc gtccccggtc gaagtccggg cctgccgcgc gcacgatcgt gtcgacgaag
     1981 cgaaacggat aggcgtgcgg gagctccggc tttgtcatca ggcgatcttc ttctgctcga
     2041 taaagtcgac gagcgcgttc acggacgcga acgccttgac cccgacctct tcgtcctcga
     2101 cctttatacc gaactcctgc tcgaggccca gcacgagctc cagtgcgtcg atcgagtcga
     2161 gccccagccc ttccccgaaa agcggagcgt cgctctcgat cgatgccggg tcgatatcga
     2221 gcttcaggcg ccgcacgatc aggtccttga ctcggggcgc caggtctgcg tttgccatgg
     2281 ggtctcctcg tgaaaattat agatgcgcca gcgggcgctc tcaggccatt cctccgtcga
     2341 cgcgccagac ctgcccggtg acgtaggaag cccgatccga cgccagaaac ccgacgaccg
     2401 ccgcgatctc ctccggcgct ccgaagcggg ccatcgggat gcgcctcagg aactcctgtc
     2461 gggcgtcctc cttcaggtgc gcgatgattt ccgtcgagac gaggcccggg gcaaccgcgt
     2521 tggccgtgat tccccgggtc gcgagctcga gcgcgagcgt cttcgtgaag gcgatcagtc
     2581 cgcccttcgc cgccgagtag ttcgcctggc ccacgtggcc ggagatgccc gagaccgaaa
     2641 cgacgttgat gatccgtccc cagcccttgt ggagcatccc gcggacgacg gccttggtga
     2701 cgcggaacgc gcccttcaag tcggtgtcca ggacgaggtc ccagtcctcc tccttcatga
     2761 gcggaaagat cgcgtccttg atgacgccag cgttgttcac cagcacgtcg gctccgccga
     2821 actcggaggt catgcggtct ccgagcgcgc gaacgtcggc cgccttcgtg acatcggacg
     2881 cgatcgcgac cgcgtctcct ccgagggcgc ggatggccgc gacggtcgcg tccgcgcggg
     2941 cgacgtcctg gcggtacgag agaacgatcc ggtgtccggt ggacgcaagc tcgagagcga
     3001 tcgcgcgccc gatccccctc gatccgccgg tgacgagcgc cgttctcatg ggcggccgag
     3061 agtatccgac accgtgcgga tggcggccag cgaccccgca ggccggcagg tcgtcgtgcg
     3121 gtcgatccag cgcgccattt tcgacagcat gtcgcccggt ccgacctcga cgaacattcg
     3181 gattccgtcg gagaccatcg cctcgtaagt gtcgttccag agcgtcgggt gaacgaactc
     3241 ggttccgagc agctcgcgaa cgcgcgcgcc cgtacgggcg cgccgcccgt cgggaccgta
     3301 atagggaacc gccgggtcgc ggatcgaacg gcaggcctcg acgaccggcg cgacggcctg
     3361 cgccaccggc tccatcagcg ggctgtgaat cggccagctc atggtgagcg ggagcaccga
     3421 gagcgacttc ggagcaagaa cccgaagcgc ctcttctacg gcttcggagc cacccgtcag
     3481 gacgaactgg gtcgacgcgt tcacgttgcc gatccacacc ggcagaccgc gggcgttcag
     3541 atcggcgcag acctcctcga cggtctcccg ccgggt
//
LOCUS       DASWAV010000514         6041 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U2665
            k141_68306, whole genome shotgun sequence.
ACCESSION   DASWAV010000514 DASWAV010000000
VERSION     DASWAV010000514.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960316
            Sequence Read Archive: SRR10260007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 6041)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6041)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:47:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,929
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6041
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_68306"
                     /isolate="SMAG_U2665"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.36444 N 132.31889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            204..1910
                     /locus_tag="VF958_10780"
     CDS             204..1910
                     /locus_tag="VF958_10780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007907729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase family protein"
                     /protein_id="HEX9149633.1"
                     /translation="MTEEKRQDPTDDPTVAPGVREGRRRWEDRGREIYSGRPAWKKDF
                     TTVSGAEVSPLAGPDSVAGFDFERDLGWPGEYPYTRGVQPTMYRGKLWTMRQFAGYGT
                     ARQTNERFHYLLEHGQTGLSTAFHLPTLYGYDSDHPMARGEVGKCGVAIDSLKDMEVL
                     FDRIDLGVVTTSMTINSTAPIALAMYLAVAEKHGTPWDRVGGTLQNDILKEYIAQKEY
                     IFPPRPSMRLITDQFSFCAAHVPRWNTISISGYHIREAGSTALQELAFTLRDGVEYVK
                     YGLEAGLAIDEFAPRLSFFFNAHNDFFEEIAKYRAARRVWAKTMRERFGAKDPRSWMC
                     RFHTQTAGCSLTAQQPYNNVIRTTLQALAGVLGGTNSLHTNSLDETLALPSDFAVKLA
                     LRTQQVIAHESGVANTIDPLGGSYFVEEMTTRMERGCFDYFRRIDALGGMVEAIEAGF
                     PQREIMDAAYAYQRAVDSKAKIVVGVNDFVEEENGDRVEILYIDESVEDEQKKELAAL
                     RRSRDGKAVTSALSALRQACADGKNVMPPLIEAVKTYATVGEISDVMREVFATYEEPA
                     VF"
     gene            1991..3208
                     /locus_tag="VF958_10785"
     CDS             1991..3208
                     /locus_tag="VF958_10785"
                     /EC_number="3.5.4.25"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012844548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003935 - GTP cyclohydrolase II activity
                     [Evidence IEA];
                     GO_function: GO:0008686 -
                     3,4-dihydroxy-2-butanone-4-phosphate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     3,4-dihydroxy-2-butanone-4-phosphate synthase/GTP
                     cyclohydrolase II"
                     /protein_id="HEX9149634.1"
                     /translation="MTSEKAPFSSIDRAVEELRAGRFVVVVDDEDRENEGDLVLAAEK
                     VTPEAINFMAREGRGLICLALTEERCGELDLPPMVDENTSTFGTAFTVSIEARGRVTT
                     GISAADRAATVKAAIDPRTRPADLARPGHMFPLRAKVGGVLKRAGQTEASVDLARIAG
                     LDPSAVICEVMNEDGTMARVPDLAAFCGKHGLVMVTVADLIRYRMKTERLVQRIAAPH
                     LPTRFGEFRLYAYRSDVTHEEHVALVHGEIDEEDPVLVRVHSQCLTGDVFGSSRCDCG
                     AQLALAMEKITEAGKGVFLYLLQEGRGIGLVNKLKAYELQDEGHDTVSANEKLGFPPD
                     IRNYGVGCQILRDLGVRKMRLMTNNPSKYVAISGYGLEIVERVPLEVRPTAGSRKYLE
                     AKKNKMGHLLKMV"
     gene            3286..4125
                     /locus_tag="VF958_10790"
     CDS             3286..4125
                     /locus_tag="VF958_10790"
                     /inference="COORDINATES: protein motif:HMM:NF013324.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005743 - mitochondrial inner membrane
                     [Evidence IEA];
                     GO_component: GO:0035632 - mitochondrial prohibitin
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prohibitin family protein"
                     /protein_id="HEX9149635.1"
                     /translation="MSKLFEVVPPLPPGRRIARVVIIVVILVLLLAGNPVAVIPAGHV
                     GVKDFFGRVSSDVLSPGVRVVFPFTRVVKMSIKTQEVKETAEVPSKEGLIMELEGSLL
                     FRLDPQKADDMYRTIGRDYQEIAVAPQIRSAIREITASYEAKVLYSAERERISRETLE
                     LFRKLAGDRGIIAEAVLLRKIGLPAVVANAIQEKLRREQEAEQMKFVLQKEQQEAERK
                     RIEAQGIADFQKIVAAGISPQLLEWKGIEATEKLAMSQNSKVVVIGSGKNGLPIILGG
                     EGK"
     gene            4122..4721
                     /locus_tag="VF958_10795"
     CDS             4122..4721
                     /locus_tag="VF958_10795"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006386070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HEX9149636.1"
                     /translation="MKGAAGLEPVVLEGRFVRLEPLSLDHFGDLAHVAFDEEIWRWTS
                     ERAMSLEELRAYVERALAASLTGSVLPFATVARRVRRAVGSTRFANFDAPNRRVEIGW
                     TWLGRDWQRTAINTEAKYLMLTHAFEKLGCVRVELRTDVLNERSRAAIRRIGASEEGV
                     LRKHAITSTGRVRDDVYHSIVDEEWPAVKAKLEARIARG"
     gene            complement(4718..5386)
                     /locus_tag="VF958_10800"
     CDS             complement(4718..5386)
                     /locus_tag="VF958_10800"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX9149637.1"
                     /translation="MLISVIVPVRDETPDVAGNFARFGLDPSCELLIADGGNRTATRD
                     AFRDAGARLLPAEGSRGARLAAAVAQARGDVLFFVHADSRPPVESLEIIRRTIAGGAD
                     AGSFSLRYEGGGPVMRWVAWWANRRSRLARLPFGDQGIFCRRDAYERAGGIRDLPICD
                     DLDFVLRLRRAGRFVVRPEMTATSPRRYVGGAPRQVLRNWAVICGYFAGVSPQKLARF
                     YEGR"
     gene            5438..6016
                     /locus_tag="VF958_10805"
     CDS             5438..6016
                     /locus_tag="VF958_10805"
                     /EC_number="2.7.7.77"
                     /inference="COORDINATES: protein motif:HMM:NF024214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0061603 - molybdenum cofactor
                     guanylyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor guanylyltransferase"
                     /protein_id="HEX9149638.1"
                     /translation="MPAAVLAGGASKRMGRPKAALPWGAGNLLEFQTGRLAALFREVI
                     VVVKQPPDFPVGPARVVLDASPEYAAIHGLVRALEETEDRMFVLAVDLPGLTHDIVHE
                     IATRGLKTPAPALVPEAGGRLQPLAAVWRRSAMRFASRRISRGMLSLTGLVEEVGAEI
                     FPERDWRALDPSGNAFANLNTLVDWAAHRERA"
BASE COUNT         1082 a         1947 c         2043 g          969 t
ORIGIN      
        1 gtctgcgcgc atgcgatcgc gaacattccc gccgcaagaa tccccagaag agtagtgatc
       61 cgtttcgcca ttgaagttcc tccttgcctg gccaaatggc cgcgtgacct tatttgcatc
      121 tttcatgccg ttcggtccga ggcggcaaga tcgttggaaa tgtctgagac gtatagggta
      181 ttctccgcgg cgggaacgac acgatgactg aagagaagcg tcaggatccc accgacgatc
      241 cgacggtcgc cccgggcgtt cgcgaaggaa ggcgccgatg ggaggaccgc ggtcgggaga
      301 tctactccgg ccgccccgcc tggaagaagg atttcaccac cgtctccgga gcggaggtca
      361 gcccgctcgc ggggccggat tctgttgcgg gcttcgactt cgagcgcgac ctggggtggc
      421 cgggcgagta tccctacacc cgcggcgttc agccgaccat gtatcgcgga aagctctgga
      481 cgatgcgcca gttcgccggg tatgggacgg cgcgccagac caacgagcgg ttccactatc
      541 tcctcgagca cgggcaaacc ggactctcga cggcgttcca tctcccgacg ctctacggct
      601 acgactcgga tcaccccatg gcccggggtg aggtcgggaa gtgcggcgtc gcgatcgatt
      661 cccttaagga catggaggtg ctcttcgacc gaatcgacct cggagtggtg acgacgtcga
      721 tgacgatcaa ctcgacggcg ccgatcgcgc tcgcgatgta cctcgccgtc gcggagaagc
      781 acggcacgcc gtgggatcgc gtcggcggga cgctgcagaa cgacatcctg aaggagtaca
      841 tcgcccagaa ggaatacatc ttcccgcccc gcccctcgat gcggctgatc accgaccagt
      901 tctcgttctg cgccgcacac gttccgcgct ggaacacgat ctcgatctcc gggtaccaca
      961 tccgcgaggc cggctcgacc gcactccagg agctcgcatt cacactgcgc gacggcgtcg
     1021 aatacgtgaa gtacggtctc gaggcgggac tcgcgatcga cgagttcgcg ccccgcctgt
     1081 ccttcttctt caatgcgcac aacgatttct tcgaggagat cgcgaagtat cgcgccgcgc
     1141 ggcgcgtctg ggcgaagacg atgcgtgagc gcttcggagc gaaagatccg cgctcctgga
     1201 tgtgccggtt ccatacgcaa accgcgggat gctcgctcac cgcgcaacag ccgtacaaca
     1261 acgtgatccg aacgacgctc caggcgctcg cgggagtcct cggcggcacg aactcgctcc
     1321 acacgaactc gctggacgag acgctggcgc tgccgagcga tttcgccgtc aagctggcgc
     1381 tgcggaccca gcaggtcatc gcacacgagt cgggcgtcgc gaatacgatc gatccgctgg
     1441 gcgggtcgta cttcgtcgag gagatgacga cccggatgga gcgcggctgc ttcgattact
     1501 tccgccggat cgacgcgctc ggcggcatgg tcgaggcgat cgaagccggg tttccgcagc
     1561 gcgaaatcat ggacgccgcc tacgcctacc agcgggcggt cgactcgaag gcgaagatcg
     1621 tcgtgggggt caacgacttc gtcgaggaag aaaacgggga ccgggtcgag atcctttaca
     1681 tcgacgagtc cgtggaggat gagcagaaga aagagctcgc cgccctgcgc cgttcgcggg
     1741 acggaaaggc agtcacttcc gcgctcagcg ccctccgaca ggcgtgcgcc gatggaaaga
     1801 acgtgatgcc tccgttgatc gaggccgtga agacgtacgc gaccgtcggc gagatctcgg
     1861 acgtcatgcg ggaagtcttc gcgacgtacg aggagccggc ggtcttctag aaacgccgtc
     1921 ggcctggcaa tcccctcacc ccggccctct cccggaggga gagggagaaa ggcgtgagaa
     1981 aatcgagctc atgaccagcg aaaaggcccc cttctcctcc atcgatcgcg ccgtcgaaga
     2041 gctccgcgcc ggacgcttcg tcgtcgtggt ggacgacgag gaccgggaga acgagggcga
     2101 cctcgtcctc gcggcggaga aggtcacccc ggaggcgatc aacttcatgg cgcgcgaggg
     2161 gcggggactc atctgcctgg ccctgaccga ggagcgctgc ggcgagctcg atctcccgcc
     2221 gatggtggac gagaacacgt cgaccttcgg cacggcgttc accgtttcca tcgaagcgcg
     2281 ggggcgcgtg acgacgggaa tctcggcggc cgaccgagcg gccacggtca aagcggcgat
     2341 cgatccccgc acgcgtcccg ccgatctcgc gcggcccggt cacatgtttc cgctgcgcgc
     2401 gaaagtgggc ggcgtcctga aacgcgccgg ccagaccgag gcgtcggtgg acctcgcgcg
     2461 gattgccggg ctcgacccgt ccgcggtgat ctgcgaagtg atgaacgagg acggcacgat
     2521 ggcgcgggtg cccgacctcg cggcgttttg cgggaagcac ggtctcgtca tggtcacggt
     2581 cgccgacctg atccgctacc ggatgaagac cgagcgcctc gtccaacgta ttgcggcacc
     2641 ccaccttccg acccgcttcg gcgagtttcg cctctacgcg tatcgcagcg acgtcacgca
     2701 cgaggagcac gttgcgctcg ttcacggcga gatcgacgag gaggatcccg ttctcgtgcg
     2761 agtgcactct cagtgcttga cgggcgacgt gttcggctcg agccggtgcg actgcggagc
     2821 gcaactcgcg ctcgcgatgg agaagatcac ggaggccggc aagggggtct tcctttatct
     2881 gctccaggag gggcgcggga tcggcctggt caacaagctg aaggcctacg agctccagga
     2941 cgaggggcac gacaccgtat cggccaacga gaagctcggt ttcccgcccg acatccgcaa
     3001 ctatggcgtc ggctgccaga tccttcgcga cctcggcgtc cggaagatgc ggctgatgac
     3061 gaacaatccg tccaagtacg tcgcgatcag cggttatggt ctcgaaatcg tcgagcgcgt
     3121 gccgctcgaa gtgcgtccga ccgcggggtc ccgcaagtac ctcgaagcga agaagaacaa
     3181 gatgggccac ctgctgaaga tggtctgaag ccgcttcgac tccggccggt ccgcgaagcg
     3241 cgcatatcct tcctccgatt cgttcctgca acaaggagga atctcatgtc gaaactgttc
     3301 gaggtcgtcc cgcccctgcc ccccggccgc cggatcgcgc gcgtcgtgat catcgtcgtc
     3361 atcctcgtcc tgcttctcgc cggcaacccg gtcgccgtca tccccgcggg ccacgtcggc
     3421 gtcaaggatt tcttcgggcg cgtctcgtcc gacgtgctct ccccgggggt tcgggtcgtt
     3481 tttccgttca cgcgcgtcgt caagatgtcc atcaagacgc aggaggtcaa agagaccgcc
     3541 gaggtgccct cgaaagaggg gctgatcatg gagctcgagg gctcgctgct gttccgtctc
     3601 gatccgcaga aggccgacga catgtatcgg accatcgggc gcgactacca ggaaatcgcg
     3661 gtcgccccgc agatccgctc cgcgatccgc gagatcacgg catcgtacga ggcgaaggta
     3721 ctttactcgg cggagcgaga gcgcatctcg agagagaccc tggaactctt tcggaagctc
     3781 gccggcgacc gcgggatcat cgcggaagcc gttctgctgc ggaagatcgg tctgccggcg
     3841 gtcgtcgcga atgcgatcca ggagaagctc cgccgcgagc aggaagcgga acagatgaag
     3901 ttcgtccttc agaaggagca gcaggaggcg gagagaaaga ggatcgaggc gcaggggatt
     3961 gcggacttcc agaagatcgt ggcggcgggg atctccccgc agctcctcga gtggaaggga
     4021 atcgaggcga cggagaagct cgcgatgagc cagaactcga aggtcgtcgt catcgggagc
     4081 ggaaagaacg gtttgccgat catcctcggc ggcgaaggaa aatgaagggg gcagcgggac
     4141 tcgaacccgt cgtcctcgaa ggccgcttcg tccgcctcga gccgctgtcg ctcgaccact
     4201 tcggtgacct cgcgcacgtc gcgttcgacg aagagatctg gcgatggacc agcgagcggg
     4261 cgatgtccct cgaagagctc cgcgcttacg tcgagcgcgc gctcgccgct tccctcacgg
     4321 gctcggttct tccgttcgcg accgtggccc gtcgcgttag aagggccgtc ggcagcacgc
     4381 gcttcgcgaa tttcgacgcc ccaaaccggc gcgtggagat cggctggacg tggctgggcc
     4441 gagattggca gcgcaccgcg atcaacacgg aagcgaagta cctcatgctg acccacgcgt
     4501 tcgagaagct cgggtgcgtc cgggtggagt tgagaacgga cgtcttgaat gagcgctccc
     4561 gagcggcgat ccgccgcatc ggcgcctcgg aggaaggcgt tctgcgaaag cacgcgatca
     4621 cctcgaccgg acgggtccgg gacgacgttt accacagcat cgtcgacgaa gagtggccgg
     4681 cggtgaaggc gaagctggag gcgcgcatcg cgaggggcta gcggccttcg tagaagcggg
     4741 cgagcttctg cggagagacg cccgcaaagt atccgcagat gaccgcccag ttgcgtaaca
     4801 cctgccgggg ggctcccccg acgtagcggc gcggggacgt cgcggtcatt tccggcctca
     4861 cgacgaaccg tccggcgcgt cggaggcgaa gaacgaagtc gaggtcgtcg cagatcggca
     4921 gatcgcggat tccgccggcg cgctcgtagg cgtcccggcg gcagaagatc ccttgatcgc
     4981 cgaagggcag ccgcgcgagg cgcgatcggc gatttgccca ccacgcaacc cagcgcatca
     5041 ccgggccccc gccttcgtac cgcagggaaa aggacccggc atcggcgccc cccgcgatcg
     5101 tccggcggat gatctcgagg gattcgacgg gaggtctcga gtccgcgtgc acgaagaaga
     5161 gcacgtcgcc gcgcgcttgc gccaccgcgg ccgccaggcg ggcgccccgg cttccctctg
     5221 cggggagaag ccgcgcgccg gcatcccgga aggcatcgcg cgtggcggtc cggttgcccc
     5281 catcggcgat caagagctcg caggaggggt cgagaccgaa gcgggcaaag ttcccggcga
     5341 cgtcgggagt ctcgtctcgg accgggacga tgacggaaat gagcaagagc ttctgcgagc
     5401 ttcggatgct agcatcgggc gtacagtgaa actcccgatg ccggcggcgg tactcgccgg
     5461 cggcgcgtcg aagcggatgg gccgtccgaa agcggcgctc ccctgggggg ccgggaacct
     5521 cctcgagttt cagacggggc gcctcgccgc tctcttccgc gaggtcatcg tcgtcgtcaa
     5581 gcagccgccc gactttccgg tcggtccggc gcgagtcgtt ctcgatgctt cgcccgagta
     5641 tgcggcgatc catggtctcg tccgtgcgct cgaagagacg gaggaccgga tgttcgttct
     5701 cgccgtcgac ctgccgggcc tgacccatga catcgtccac gagatcgcca cccgcggcct
     5761 gaagaccccg gccccggctc tcgtgccgga ggcgggcggg cgcctgcagc cgctcgcggc
     5821 ggtgtggcgg cgctccgcga tgcgtttcgc cagtcggcgg atttcccgcg ggatgctgtc
     5881 gctgaccgga ctcgtcgaag aggtcggcgc ggagatcttt ccggagcgcg attggcgcgc
     5941 gctcgatccg agcggcaacg catttgccaa cctgaacacg ctcgtggact gggccgccca
     6001 ccgggagcgc gcttgagcgc ccggcggcgc gccggcaagc g
//
LOCUS       DASWAV010000515         1648 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U2665
            k141_150737, whole genome shotgun sequence.
ACCESSION   DASWAV010000515 DASWAV010000000
VERSION     DASWAV010000515.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960316
            Sequence Read Archive: SRR10260007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 1648)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1648)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:47:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,929
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1648
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_150737"
                     /isolate="SMAG_U2665"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.36444 N 132.31889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..501
                     /locus_tag="VF958_10810"
     CDS             <1..501
                     /locus_tag="VF958_10810"
                     /inference="COORDINATES: protein motif:HMM:NF038402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helical backbone metal receptor"
                     /protein_id="HEX9149639.1"
                     /translation="KKVAEVAPEIRAWGARLGEEEQADALASRIEEDLAALERTPAPT
                     TFRYAYWIWRNPWMTVSDDTYVADLLRLAGGVNAFGGEASRYPEASPEEALERGALLH
                     FFPSEPYPFRAEKHAEAVARIFGEQSRRLFVEGDDLCWHGFRTLHGLAAARRLRVYAE
                     PASPAG"
     gene            complement(485..1102)
                     /gene="folE"
                     /locus_tag="VF958_10815"
     CDS             complement(485..1102)
                     /gene="folE"
                     /locus_tag="VF958_10815"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003934 - GTP cyclohydrolase I activity
                     [Evidence IEA];
                     GO_process: GO:0042559 - pteridine-containing compound
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0046654 - tetrahydrofolate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="HEX9149640.1"
                     /translation="MAHPPIDEVELLAPSATDAEKIRVIAHHMRSIIEALGLDPRDPN
                     LHETDERVAKMYLEMFHGLSEGAEPKVTVFPNEEGYTAMVMEKDIPFYSMCAHHFVPF
                     YGHAHIAYIPNEKIVGLSKMPRIVEFYAHRPQLQERLTEQIAGFMAEKLSPQGVMVVI
                     EARHLCVEMRGVKKPGALTVTSAIRGIFFNRPVREEFLDLLNRRG"
     gene            complement(1145..>1648)
                     /locus_tag="VF958_10820"
     CDS             complement(1145..>1648)
                     /locus_tag="VF958_10820"
                     /inference="COORDINATES: protein motif:HMM:NF024110.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX9149641.1"
                     /translation="RGTLEQLMVTPASPVAVVLGKLIPYLVLAFLQLLLVLVLMTTVF
                     RVPIHGSVPLLLLLSIVYLFALLALGLLVSSWSATQMEAIQRTQMFLLPSIMLSGYIF
                     PLSSLPVPLRVVAQLLPATHFIKISRGIIIRGAEFFDLWPSVVALIALALVLVAGSTR
                     AFRKTIS"
BASE COUNT          304 a          492 c          602 g          250 t
ORIGIN      
        1 aagaaagtcg ccgaggtggc gccggagatc cgcgcctggg gcgcccgtct cggcgaggag
       61 gagcaggcgg acgctctcgc gtcgcggatc gaggaggatc tggcggctct cgagcggacg
      121 cccgcgccga cgacgtttcg atacgcgtac tggatctgga gaaacccgtg gatgacggtg
      181 tcggacgaca cgtacgtcgc cgacctgctg cgactggctg gcggcgtcaa cgcgttcggc
      241 ggtgaagcga gtcgctatcc cgaggcaagc cccgaggagg cgctcgagcg gggagcgctc
      301 ctgcacttct ttccgagcga gccctatccg ttccgcgcgg agaagcacgc ggaggcggtc
      361 gcccggatct tcggagagca gtcgcggcgc ctcttcgtgg agggcgacga cctgtgctgg
      421 cacggcttcc ggacgctcca cggccttgcg gcggcccgca ggctgcgcgt ctacgccgag
      481 cctgctagcc ccgccggttg agcaggtcca gaaactcctc gcgcaccggc cggttgaaga
      541 agatcccgcg gatcgcggag gtcaccgtga gcgcgcccgg cttcttcacg ccgcgcatct
      601 cgacgcacag gtgccgcgcc tcgatgacga ccatcacgcc ctgcggagat agtttctccg
      661 ccatgaagcc cgcgatctgc tcggtgaggc gctcctggag ctgcgggcgg tgcgcgtaga
      721 actcgacgat gcgcggcatc ttggagagtc cgacgatctt ctcgttgggg atgtaggcga
      781 tgtgggcatg gccgtagaag ggaacgaagt ggtgagcgca catggagtag aacgggatgt
      841 ccttctccat gaccatcgcc gtatagccct cttcgttcgg aaagacggtc accttcggct
      901 ccgcgccctc ggaaagcccg tgaaacatct cgaggtacat cttcgcgacg cgctcgtcgg
      961 tctcgtgaag gttggggtcc cgcggatcga ggccgagggc ctcgatgatc gagcgcatgt
     1021 gatgagcgat gacccggatc ttctccgcat ccgtcgcgga cggggcgagg agttcgactt
     1081 cgtcgatcgg gggatgtgcc atgcccggaa tcttagccga gagcgggccg cgcgcgggtc
     1141 ttctctagga gatcgtcttg cggaaggcgc gggtcgagcc ggccaccagg acgagggcga
     1201 gcgcgatcag cgcgacgaca ctcggccaga ggtcgaagaa ctccgcgccg cggatgatga
     1261 tcccgcggga gatcttgatg aagtgcgtgg cgggcaggag ctgggcgaca acgcgcagcg
     1321 gcaccggcag cgaagagagc ggaaagatgt atccggagag catgatcgac gggagcagga
     1381 acatctgcgt ccgctggatc gcttccatct gcgtcgcgga ccaggaggaa acgagcagcc
     1441 cgagcgcgag gagcgcgaac aggtagacga tcgagagtaa gagcagcagc ggcacgctgc
     1501 cgtggatcgg gacgcgaaag accgtcgtca tgagcacgag gacgaggaga agctggagga
     1561 aggcgagaac gaggtagggg atgagcttgc cgagcacgac cgccacggga gacgccggcg
     1621 tgaccatgag ctgctcgagc gtcccccg
//
LOCUS       DASWAV010000516         1533 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U2665
            k141_190125, whole genome shotgun sequence.
ACCESSION   DASWAV010000516 DASWAV010000000
VERSION     DASWAV010000516.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960316
            Sequence Read Archive: SRR10260007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 1533)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1533)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:47:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,929
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1533
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_190125"
                     /isolate="SMAG_U2665"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.36444 N 132.31889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..468)
                     /locus_tag="VF958_10825"
     CDS             complement(<1..468)
                     /locus_tag="VF958_10825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9149642.1"
                     /translation="MRKLGIYSILVSCLLLVASVSLAQSVKGENVVVGKDAITTKLAD
                     GKTYVTQGNHQVFLTADPKHPLNGASGDCDGACVMDASNVAMCMGSCTQVDREGDIAF
                     FTWDGQTEGGWKLAGGSGKWKGATGQGAWKNSVAAAPGNFARIAWEGTMSMGKK"
     gene            complement(731..1303)
                     /gene="amrA"
                     /locus_tag="VF958_10830"
     CDS             complement(731..1303)
                     /gene="amrA"
                     /locus_tag="VF958_10830"
                     /inference="COORDINATES: protein motif:HMM:TIGR00296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system protein A"
                     /protein_id="HEX9149643.1"
                     /translation="MSEPFPPLSKEARRQLLALARGTLEADFRGEAPPRLGSDRAETF
                     GEPRALFVTLRRDGQLRGCIGTLAPEGDLSRSVPRFARQAAFADPRFPGLTAEELPAC
                     TIEISVLTPPRPVEDPHEIVVGRDGLIIEFGGRRGLLLPQVATEWGFDRERFLFEVAR
                     KAGLPPDAWRLPGAQLWSFQAEVFAEGEET"
     gene            complement(1300..>1533)
                     /locus_tag="VF958_10835"
     CDS             complement(1300..>1533)
                     /locus_tag="VF958_10835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9149644.1"
                     /translation="PLASDVGADLPFPKVPALFVVGKDDVFGPPARLREWAAGRGLVV
                     EVAAADHVLEGKLDELESAIADFLVGIVGARTA"
BASE COUNT          244 a          536 c          502 g          251 t
ORIGIN      
        1 cttcttcccc atcgacatcg tgccttccca ggcgatgcgc gcgaagttgc cgggcgccgc
       61 agcgacgctg ttcttccacg cgccctgacc cgtcgcgccc ttccatttcc ccgagccgcc
      121 ggcgagcttc cacccgccct cggtctgccc gtcccacgtg aagaaagcga tgtcgccttc
      181 gcggtccacc tgcgtgcagg atcccatgca catcgcgacg ttgctcgcat ccatcacgca
      241 ggcgccgtcg caatcgcccg aagcgccgtt caacggatgc ttcgggtccg ccgttaagaa
      301 gacctgatgg ttcccctgtg tcacgtaggt ctttccgtcc gcgagcttgg tcgtgatcgc
      361 gtccttgccg acgacgacgt tctcgccctt cacggactgg gcgagcgaaa ccgacgcgac
      421 caggagcaga cacgagacga gaatcgagta aatgccgagt tttctcatgg gttcctccaa
      481 cagtcggatc ccgcgcggtg tgattttgga ttgacggagt ccgagctgcc ggccagtcta
      541 tcccggtttt ctggagccgt ccgagtcgcg gcgcgagggc gcgggtcggg gcggagtcgg
      601 tcgtaccgga cgctgcccac ccctctcgcc tcggggagcc acgatcttct tctggcggtt
      661 gccggtgccg cgtcgcgtca ttgctcgatc gcggcgtgtg cgaagcagac acgccgcctc
      721 aggtaccctt tcacgtctcc tcgccttccg cgaacacctc ggcctggaac gaccagagct
      781 gcgcgccggg gagccgccac gcgtcgggcg gcaacccggc cttgcgtgcg acctcgaaga
      841 gaaacctctc ccgatcgaat ccccactctg tcgcaacctg cggcagcagg agcccacgcc
      901 ggccgccgaa ctcgatgatc aggccgtcgc ggccgacgac gatctcgtga gggtcctcga
      961 cgggacgagg aggcgtcagg accgagatct cgatcgtgca cgcgggaagc tcctccgcgg
     1021 tcaggccggg gaagcgcgga tccgcgaacg cggcctggcg agcgaatcgc gggaccgagc
     1081 gggagaggtc cccctcggga gcgagcgtgc cgatgcagcc gcgcagctga ccatcccgtc
     1141 gcagcgtgac gaagagggcc cggggttcac cgaaggtctc cgcccggtcg gagccgaggc
     1201 gcggcggcgc ctcaccgcgg aagtcggctt cgagagttcc gcgcgcaagg gcgagcagct
     1261 gtcgtcgggc ctctttcgag aggggcggga aaggttcgct catgcggtcc tggctccgac
     1321 aatacccacg aggaaatcgg cgatcgcgct ctcgagctcg tccagctttc cctccaggac
     1381 gtggtccgct gccgccacct cgacgacgag gcctctcccg gccgcccact cgcggagccg
     1441 cgcgggagga ccgaagacat cgtcttttcc gacgacgaag agagccggca ctttcgggaa
     1501 cgggagatcg gcgccgacat cggatgcgag cgg
//
LOCUS       DASWAV010000517         3400 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U2665
            k141_749883, whole genome shotgun sequence.
ACCESSION   DASWAV010000517 DASWAV010000000
VERSION     DASWAV010000517.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960316
            Sequence Read Archive: SRR10260007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 3400)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3400)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:47:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,929
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3400
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_749883"
                     /isolate="SMAG_U2665"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.36444 N 132.31889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..434
                     /locus_tag="VF958_10840"
     CDS             <1..434
                     /locus_tag="VF958_10840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011984570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="nitrous oxide reductase family maturation
                     protein NosD"
                     /protein_id="HEX9149645.1"
                     /translation="SENRFEGNSFIENLSPLEFVGRRTDTRFDGNYWSGNDVPDLDGD
                     GFSDREYRLSSVFDHFRGNLTAADLFTQGLGAAALAAAERALPVLDPAPVADRSPLAR
                     PPRLADVPRPTASAAGPDAAGLAVSTLAVAVGMLVFARGSR"
     gene            431..1357
                     /locus_tag="VF958_10845"
     CDS             431..1357
                     /locus_tag="VF958_10845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011421451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX9149646.1"
                     /translation="MIRFESLTKRYGATEAVVSLTFSVERGEAVALLGPNGSGKTTSL
                     KAAAGLIRPTGGRVEVGDPLEDAARPSARRTISYLPQKVAFPDALTGREVLEFYRALR
                     SAPLGRTAEVLNFASLNGASNRAVATYSGGMVQRLGLAVALLPNAPVLLLDEPTAALD
                     PDGLCAFYGLVERRRREGHTLLFTSHQMGDVERLADRLLLLVEGRLAASLSQGELARR
                     LGERGVMRLSLDRRPDGLLERVRAISPTAAWAAEELVVPGPAWERPRVIELVTASGVE
                     IRNLVAREGRLDELYRELLPGFGEPSEPKNAT"
     gene            1378..1833
                     /locus_tag="VF958_10850"
     CDS             1378..1833
                     /locus_tag="VF958_10850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9149647.1"
                     /translation="MPEPEPADGAKMEHDRLRRELEKATTIGGETADAARAVLKVLSP
                     HMLLEEEFAIPPLRLLPRLARGEFTSDMERIIPKSEMMKAELPRMLNEHALIVDALRG
                     LLQAALKERHEGYATFAQKLIFHAQQEEEFFYPASILVGEYLKLKLGRS"
     gene            1830..2672
                     /locus_tag="VF958_10855"
     CDS             1830..2672
                     /locus_tag="VF958_10855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012632841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HEX9149648.1"
                     /translation="MKSGALAAVGLCARQELVLSARSRWTQIFAAVFAALSIAVAASG
                     YVLSGGGGFEDFARTATSLASLVVLLVPVTALTIGVLQLAPERGAAEMLYSQPVARRA
                     ILLGRVLGLFIALAGAQTIGLGAAGLVIFWNAGGDGLAAYGLLAVASYALTAIFLGIA
                     ALLGAGGAIRRSRALAFALIVWFVSVVLFDVVVLAIATLLPSGYASRLLVTAVVVNPA
                     DAIRTATLLAIHGKAAFGAASLALLRLAGGPAGAGLLLAASALFWTLAPLALAGRRIE
                     RADL"
     gene            complement(2728..>3400)
                     /locus_tag="VF958_10860"
     CDS             complement(2728..>3400)
                     /locus_tag="VF958_10860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9149649.1"
                     /translation="GLRRELTPMNGLFLALAGAAVTVGSLHTAAPDHWVPFVALGRAR
                     DWSSRRTMGVTLLCGFGHVTVSALFGLAGLFFGVTLIKSVGERMSALAPLLLIGFGVL
                     YALWGLKRAFAPQLHGHPHRHYDHVHPESRTSVWTLFLLFSADPCVAVVPLMFAAAPL
                     GLGRTLGIVFLYEAATLATMLALVLSARAGARLLRTPALERYGDAAAGGVIAAIGLAV
                     VLLGI"
BASE COUNT          504 a         1216 c         1158 g          522 t
ORIGIN      
        1 gctcggaaaa ccggttcgag ggaaactcgt tcatcgagaa cctgtcgccg ctcgagttcg
       61 tcggccgccg caccgatacg cgattcgacg gcaactactg gtcgggaaac gacgtgccgg
      121 atctcgacgg ggacggattc agtgatcgcg agtaccggct ctcatcggtc ttcgatcact
      181 tccggggaaa cctgacggcc gcggatctct tcacacaggg cctcggcgcg gcggcgctcg
      241 ccgcggccga acgcgcgctt ccggtgctcg acccggcgcc ggtcgcggac cggagccctc
      301 tcgcgcgtcc accgcggctc gccgacgtac ctcgtcccac cgcctccgcc gccggaccgg
      361 atgccgcggg actcgcggtc tcgacactcg cggtggccgt gggcatgctc gtgttcgcgc
      421 ggggcagccg ttgatccgct tcgagagcct cacgaaacgc tacggcgcga ccgaggccgt
      481 cgtctcgctg acgttctccg tcgagcgcgg cgaggccgtg gcgctgctcg gcccgaacgg
      541 ctcgggcaag acgacctcgc tcaaggccgc cgcaggcctc atccgcccga ctggagggcg
      601 cgtcgaggtc ggcgatcctc tcgaggacgc cgcgaggccg tcggcccgac ggaccatctc
      661 ctatctccct cagaaggtcg cattccccga cgccctcacc ggtcgcgagg tcctcgagtt
      721 ctatcgagcc ctccgatcgg cccccctcgg ccgcaccgcg gaggtgctca atttcgcgtc
      781 gctgaacgga gcctccaacc gcgcggtcgc gacgtactcg ggcgggatgg tccagcgcct
      841 gggtctcgcc gtcgctctgc ttccgaacgc gcccgtgctc ctcctcgacg agccgaccgc
      901 ggcgctcgac cccgacggac tctgcgcgtt ctacggcctc gtggagcgcc gccggcgcga
      961 gggtcacacg ctgctcttca cctcgcacca gatgggggac gtcgagcgtc tcgccgatcg
     1021 actgctcctg ctcgtggagg gacggctcgc cgcctcactt tcgcaggggg agctcgcgcg
     1081 tcggctcggg gagcgtggcg tgatgcggct gtcgctcgat cggcgacccg acgggctgct
     1141 cgaacgggtg cgcgcgatct cgccgaccgc ggcttgggcc gcggaagagc tcgtcgtccc
     1201 cggcccggca tgggagcggc cccgcgtgat cgagctcgtg accgcgagcg gcgtggaaat
     1261 tcgaaacctg gtcgcccgcg aaggccggct ggatgagctc tatcgggagc tccttcccgg
     1321 atttggcgag ccctccgaac cgaagaatgc gacatgatga gcatggaagg agtctcaatg
     1381 ccagaacccg aaccggcgga cggagcaaag atggagcatg acaggctccg gcgcgaactc
     1441 gaaaaggcga cgacgatcgg gggggagacc gcggatgccg cgcgggcggt gctcaaggtc
     1501 ctttcgcctc acatgctgct cgaggaggag ttcgccatcc ctccgttgag actgctcccc
     1561 cggctcgcgc ggggagagtt cacctccgac atggaacgga tcattcccaa gtcggagatg
     1621 atgaaggccg agctcccccg catgttgaac gagcacgctc tcatcgtcga cgcgctgcgc
     1681 gggctcctgc aggcggcgtt gaaggaacgc cacgagggct acgcgacctt cgcgcagaag
     1741 ctgattttcc acgcgcagca ggaggaggag ttcttttacc ccgcctcgat cctggtcggc
     1801 gagtacctga agctgaagct cggacgctcg tgaagtccgg cgctctcgcg gcggtcggcc
     1861 tctgcgcgcg gcaggagctc gtcctctccg cgaggtcgcg gtggactcag atcttcgcgg
     1921 ccgttttcgc cgcactctcc atcgccgtcg ccgcctcggg ttacgtcctt tcggggggcg
     1981 gaggcttcga ggacttcgcg cgaaccgcga cgtcgctcgc gagcctggtc gttctcctcg
     2041 tcccggtcac cgcgctcacg atcggcgtcc tccagctcgc tccggagcgc ggggcggccg
     2101 agatgctgta ctcccagccc gtcgcgcggc gagccatcct cctcgggaga gtcctcggcc
     2161 tcttcatcgc cctcgcgggc gcgcagacga tcggcctcgg agcggccggg ctcgtcattt
     2221 tctggaacgc ggggggcgac ggactcgccg catacggatt gctcgccgtc gcgtcgtacg
     2281 cgctgaccgc gatctttctc ggcatcgccg cgctcctcgg cgcggggggc gccatccggc
     2341 gttcgcgcgc cctcgcattc gctctcatcg tgtggttcgt gtccgtcgtt ctcttcgacg
     2401 tcgtggtcct cgcgatcgcg accctgctgc cctccggcta cgcgtcgcgg ctcctcgtga
     2461 cggccgtcgt cgtcaacccg gccgacgcga tccggacggc gaccctcctc gcgatccacg
     2521 gaaaggccgc gttcggcgcg gcgtcgctcg cgctcctccg tctcgcgggt ggaccggccg
     2581 gcgccggact cctcctggcg gcctccgcgc tcttctggac gctcgccccc ctggcgctcg
     2641 cgggccgccg gatcgaacgc gccgacctct agcccctcga gccgccgcga ggaaccgaag
     2701 gacgcggtct gcgagacgcg ccgacgctca gattcccagc aggacgaccg cgaggccgat
     2761 ggcggcgatg acgccccccg ccgcagcatc cccatatcgc tcgagtgccg gtgtgcggag
     2821 cagccgggca ccggcgcgcg cggaaagcac cagcgcgagc atcgtggcga gggtcgcggc
     2881 ctcgtagagg aacacaatcc cgagcgtcct tccgagtccg agcggcgccg ccgcgaacat
     2941 cagcggcacg accgccacgc agggatccgc cgagaagagc aggaagagcg tccacacgct
     3001 cgtgcgggac tccgggtgca cgtggtcgta atggcgatga ggatggccgt ggagttgcgg
     3061 tgcgaacgcc cgcttcaacc cccagagggc atagagaacg ccgaagccga tcaggaggag
     3121 gggcgcgagc gcgctcatcc gctcgccgac ggacttgatc agggtgacgc cgaagaagag
     3181 gcccgcgaga ccgaagagcg cggaaacggt gacgtggccg aatccgcaca acagggtgac
     3241 gcccatggtc cggcggctcg accagtcacg cgcccgaccg agcgcgacga acggaaccca
     3301 gtgatcgggc gcggccgtgt ggagcgatcc gacggtcacc gccgcgccag ccagagcgag
     3361 aaagagaccg ttcatcggcg tcagctcccg acgtaggccc
//
LOCUS       DASWAV010000518         2017 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U2665
            k141_108941, whole genome shotgun sequence.
ACCESSION   DASWAV010000518 DASWAV010000000
VERSION     DASWAV010000518.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960316
            Sequence Read Archive: SRR10260007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2017)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2017)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:47:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,929
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2017
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_108941"
                     /isolate="SMAG_U2665"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.36444 N 132.31889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..902)
                     /locus_tag="VF958_10865"
     CDS             complement(<1..902)
                     /locus_tag="VF958_10865"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX9149650.1"
                     /translation="MRRIRFFFLVGMLGVTVGLATFACAKKEVPAPVAQAQAQTPPSN
                     GGKIPVTTASAEAKTEFLQGRDLAEKLRITDSHAHFEKAASLDPHFALAELNLANSAP
                     TGKEFFEHLGKAVSLADNASNGEKLLILATEAGANNNPAKQKDYLDQLVAAYPRDERA
                     HFNLGGYYFGQQDFPQAIEHYRKATEIAPSYSSAWNLLGYAYRQNVDYPNAETAFQKY
                     IELIPGDPNPYDSYAELLLKEGKFDDSITQYRKALSIDPNFLNAHQGIAADLMYSGKA
                     REAETELANITKKARTDGERRTAL"
     gene            complement(1014..>2017)
                     /locus_tag="VF958_10870"
     CDS             complement(1014..>2017)
                     /locus_tag="VF958_10870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011758207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF885 domain-containing protein"
                     /protein_id="HEX9149651.1"
                     /translation="ARATVGAGDLEGGREYYAYLVRHFTTLDRTPDEIHALGLEEVRR
                     IRAEMEEVIRKTEFSGGFADFLKFLRTDPRFYAKTPEELLKQASWIAKRIDGKLPSLF
                     GRLPRQPYGVEPVPAEIAPKYTGGRYVGAPPGGKKAGTYWVNTYALESRPLYVLESLT
                     LHEAVPGHHLQGALSAELTDLPPFRRFLYVDAFGEGWGLYSERLGLEMGLYTDPYSNF
                     GRLTYEMWRACRLVVDTGLHAKGWTRQQAIDYLASNTALSLHECTTETDRYISWPGQA
                     LAYKMGELKLRELRRRAEEALGARFDVREFHDRILGGGTVTLPILDARIDSWIRSKR"
BASE COUNT          309 a          635 c          688 g          385 t
ORIGIN      
        1 aacagcgcgg tccgacgttc cccgtcggtg cgcgctttct tcgtgatgtt ggcgagctcc
       61 gtctccgctt cgcgcgcctt tcccgagtac atcaggtccg cggcgattcc ctgatgcgca
      121 ttgaggaagt tcggatcgat cgacagggcc ttgcggtact gggtgatcga gtcgtcgaac
      181 ttgccctcct tcaggagcag ctcggcgtac gagtcgtaag ggttggggtc cccggggatc
      241 agctcgatgt acttctgaaa cgccgtctcc gcattcgggt agtcgacatt ctgccggtag
      301 gcgtaaccca ggaggttcca ggcggaggag taactcggag cgatctcggt cgccttcctg
      361 tagtgctcga tcgcctgcgg gaagtcctgc tgcccgaagt aatagccacc caggttgaag
      421 tgcgcccgct cgtctctcgg gtacgccgcg acgagctggt cgaggtagtc cttctgcttc
      481 gccggattgt tgttcgcgcc ggcctcggtg gccaggatga ggagcttctc gccgttggag
      541 gcgttgtccg ccaggctcac ggccttgccg aggtgctcga agaactcctt gccggtcggc
      601 gccgagttgg cgaggttcaa ctccgccagc gcgaagtgtg gatcgagcga agcggccttc
      661 tcgaagtgcg cgtgggagtc ggtgatgcgg agtttctccg ccagatcccg cccttgcagg
      721 aattcggtct tcgcctcggc tgacgccgtc gtcaccggaa tctttccccc attggacggg
      781 ggcgtctggg cctgcgcctg cgcgacagga gccggcacct ccttcttcgc gcaggcgaat
      841 gtcgcgagcc cgacagtcac tccgagcatc ccgaccagaa agaagaatcg aatccggcgc
      901 atggctcctc cttttgctgg tctggactta cgaaaatccg cccgaagggt tgagtggacg
      961 ttcgtcactc gaattggtgc ttacgtccct cccggcggcg gatcgggccc cggtcagcgc
     1021 ttcgagcgga tccaggaatc gatgcgcgcg tcgaggatcg gcagcgtcac ggtgccgccg
     1081 ccgaggatcc ggtcgtggaa ctctcgcacg tcgaaccggg cgccgagtgc ttcctcggcg
     1141 cggcgacgca actcgcgcaa cttgagctcc cccatcttgt acgcgagcgc ctggcccggc
     1201 caggagatgt agcggtcggt ctcggtcgtg cactcgtgga gtgaaagagc cgtgttcgac
     1261 gccaggtagt cgatcgcctg ctgccgcgtc cagcccttgg catgaagacc ggtgtccacg
     1321 acgagccggc acgcgcgcca catctcgtag gtcagccgcc cgaagttgct gtagggatcg
     1381 gtgtagagac ccatctccag gccgagccgc tccgagtaga gtccccagcc ctcgccgaac
     1441 gcgtcgacgt agaggaagcg ccggaaagga gggagatccg tcagctccgc cgagagcgcg
     1501 ccctggaggt ggtggcccgg gaccgcctcg tgaagtgtga gtgactcgag gacgtagagc
     1561 ggccggcttt cgagcgcgta ggtgttgacc cagtacgtgc cggccttctt cccgcccggc
     1621 ggggctccga cgtagcggcc gcccgtgtac ttgggcgcga tctcggcggg aacgggctcg
     1681 acgccgtagg gctggcgcgg caggcgtccg aagagcgagg gcagttttcc gtcgatgcgc
     1741 ttggcgatcc acgacgcctg cttcaggagc tcctcgggcg tcttcgcgta gaagcgcggg
     1801 tcggtgcgca ggaacttcag gaagtccgcg aatccgccgg aaaactcggt cttgcggatg
     1861 acttcttcca tctcggcacg gatgcggcgg acctcttcga ggccgagggc gtggatctcg
     1921 tcgggcgtgc ggtcgagcgt cgtgaagtgc cggacgagat aagcgtagta ctcccggccg
     1981 ccttccaggt cgcccgcgcc gaccgtcgcg cgggctc
//
LOCUS       DASWAV010000519         2075 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U2665
            k141_668376, whole genome shotgun sequence.
ACCESSION   DASWAV010000519 DASWAV010000000
VERSION     DASWAV010000519.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960316
            Sequence Read Archive: SRR10260007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2075)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2075)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:47:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,929
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2075
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_668376"
                     /isolate="SMAG_U2665"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.36444 N 132.31889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..531
                     /locus_tag="VF958_10875"
     CDS             <1..531
                     /locus_tag="VF958_10875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9149652.1"
                     /translation="SIAVSPKYENGFFRLGEVRELRGNLAGAEEAFAEGLAKNPKSTP
                     LAFRLAKLRSRLGRPSADADWRRALALAPGAAAFHLGYAQFLAGQGRFPAARREAREV
                     LRRQPRELGALAILADSSAVAGQRFSEGLAAEKIFRVTRSREDFDRLVRIAAADPSYR
                     TRFAALQPSLRKILAQ"
     gene            complement(525..1133)
                     /locus_tag="VF958_10880"
     CDS             complement(525..1133)
                     /locus_tag="VF958_10880"
                     /inference="COORDINATES: protein motif:HMM:NF021363.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2062 domain-containing protein"
                     /protein_id="HEX9149653.1"
                     /translation="MKFPIPRAFRRRRKHPLKLSRRWKVLLLDLLGREEPPERVAAAI
                     ALGIGVGFSPFMGIHFLIAIGLAFLFRLNRIDALLGTLVGNPWTLPPVYAAGYALGRQ
                     LLNYDRRKVPDLHWHRLLHRDFWHAFSGSALRPRLASYVVGTAVIGLLIGLSSYLVIR
                     ALLRIYHRRHPRVAVRAARLRDRAARRKKRRSEMRTDPIDDY"
     gene            complement(1130..>2075)
                     /gene="thiL"
                     /locus_tag="VF958_10885"
     CDS             complement(1130..>2075)
                     /gene="thiL"
                     /locus_tag="VF958_10885"
                     /EC_number="2.7.4.16"
                     /inference="COORDINATES: protein motif:HMM:TIGR01379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009030 - thiamine-phosphate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="thiamine-phosphate kinase"
                     /protein_id="HEX9149654.1"
                     /translation="DDAAVIGIEAGPVAVTTDTLVEGIDFLPGEPPARLGRRAVSVAL
                     SDLAAMGAWPDFILSTLGLPSSTAPDFPLAVSLAAARRAGDFGAVLAGGDLSKATELF
                     LTVAAWGRPAGDPILRSTARPGDALFVSGWPGEAGAGLAVARARAGLDPGPGEGGWTP
                     EMLPVAHETRLLAAFRDPEPRLALGRALSRDRLATAAIDVSDGLGVDAARLARASGVC
                     LVIERAELPLSPALRSFADVAGGDPLELALGAGDDYELLFTVAPENVGRTADPPAEWG
                     VEVRRIGRVEAGEGAFLEDENGRRPIGALGFDHLEGPR"
BASE COUNT          335 a          735 c          742 g          263 t
ORIGIN      
        1 tcgatcgcgg tctccccgaa gtacgagaac gggttctttc ggctcggaga agtgcgcgag
       61 ctgcgaggaa atctcgccgg cgccgaagaa gctttcgcgg aaggcctcgc gaaaaacccg
      121 aagtcgaccc cgctcgcgtt ccgcctggcg aagctacgtt ccaggctcgg acggccgagc
      181 gccgacgcgg actggcggcg cgcgctcgct ctcgcacccg gcgccgccgc gttccacctg
      241 gggtatgcgc agtttctcgc ggggcagggg agattccccg cggcgcgaag agaggcgcga
      301 gaggtgctcc gccgacaacc gcgcgagctc ggcgcgctcg cgatcctcgc cgactcgagc
      361 gcggtcgccg gccagcgctt ctccgaaggt ctggccgccg aaaagatctt ccgcgtcacg
      421 cggtcccggg aggatttcga tcggctcgtg cggatcgcgg ccgccgaccc ttcctaccgg
      481 acccgcttcg ccgctctcca gccctcgctc cggaaaattc tggctcagta gtcgtcgatc
      541 ggatcggtcc gcatctcgct gcgtcgcttc ttacggcgcg ccgcacggtc ccgcaagcgc
      601 gccgcccgca ccgccaccct cgggtgccgg cggtggtaga tccgaagcag cgctcggatc
      661 acgagatagg acgacaaccc gatcaggagt ccgatgacgg cggtccccac gacgtacgac
      721 gcgagccgcg gacggagggc cgatccgctg aacgcgtgcc agaagtcgcg gtgcaggagc
      781 cggtgccagt gaaggtccgg caccttcctg cgatcgtagt tgaggagctg ccttccgagg
      841 gcgtaacccg ctgcgtacac cgggggcagg gtccacggat ttccgacgag cgtccccagg
      901 agggcgtcga tgcggttcag ccggaagaga aaagcgaggc cgatcgcgat gaggaaatga
      961 atccccatga aaggggagaa gccgacgccg atcccgagcg cgatggcggc ggccacccgc
     1021 tcgggcggct cctcgcgccc gaggaggtcg agaaggagga ctttccaccg ccgcgagagc
     1081 ttcagaggat gcttgcgccg ccggcgaaag gcgcgcggga tcggaaactt catcgcggac
     1141 cttcgaggtg atcgaagccg agggctccga tcggacgccg gccgttttcg tcttcgagga
     1201 aggcgccctc gcccgcttcc acgcgaccga tcctccggac ttcgacgccc cactccgcgg
     1261 gcggatccgc cgtgcggccc acgttctccg gcgccaccgt gaagagaagc tcgtagtcgt
     1321 cgccggctcc gagcgcgagc tcgagcgggt cgccccccgc gacgtcggca aacgagcgca
     1381 gagccgggga gagaggaagc tccgcgcgct cgatcacgag acagacgccg gacgcgcgcg
     1441 cgagccgcgc ggcgtcgact cccagcccgt cggagacgtc gatcgccgcg gtggcgaggc
     1501 ggtcgcgcga gagggcgcgg ccgagcgcga gccgcggctc cgggtcacga aacgcggcga
     1561 ggagccgagt ctcgtgagcg acgggaagca tttccggcgt ccagccgccc tcgcccggcc
     1621 ccgggtccag gcccgcgcgc gcacgggcga ccgcgaggcc cgccccggct tctccgggcc
     1681 atcccgagac gaagagcgcg tctcccggcc gggcggtgga gcgcaggatc gggtctcctg
     1741 ccgggcgacc ccaggcggcg accgtcagga acagctcggt ggctttcgac aagtctcccc
     1801 cggcgagcac ggctccgaag tcgccggcgc ggcgcgcggc ggcgagggac accgccagcg
     1861 gaaagtcggg tgccgtcgac gacggcaacc cgagcgtcga cagaatgaag tccggccagg
     1921 cgcccatcgc cgccaggtcg gagagtgcca cggacaccgc gcggcgcccc agccgcgccg
     1981 gaggttcccc cgggagaaag tcgatgccct cgacgagggt gtccgtcgtg acggcgaccg
     2041 gaccggcctc tatgccgatg accgcggcgt cgtca
//
LOCUS       DASWAV010000520         6872 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U2665
            k141_509850, whole genome shotgun sequence.
ACCESSION   DASWAV010000520 DASWAV010000000
VERSION     DASWAV010000520.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960316
            Sequence Read Archive: SRR10260007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 6872)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6872)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:47:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,929
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6872
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_509850"
                     /isolate="SMAG_U2665"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.36444 N 132.31889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..911
                     /locus_tag="VF958_10890"
     CDS             <1..911
                     /locus_tag="VF958_10890"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HEX9149655.1"
                     /translation="ERKADLSPKITSRITELNVTEGTRVRKGDIVARLDHTDLDAQLA
                     EKRAAWVNAKAELARQRSLHDEGLAPKSTLDAAVATEATTRAAVRYVEALLDYTVIRA
                     PFAGVVTAKRAFVGEAVSPFGSSPSGGGSGGAIVTLVEFSSLYVGADVNEANLSKLGP
                     NQPAEITLDAVPDRTYHGSLRQIVPAADRQKATVRVKVAFLDADDKILPDLSARVSFT
                     SQPTQGKTGRTRVVVPASAIHSVDGKTGVFRIVDGHARFQAVTAGPEADGQVEIRQGL
                     AGGERLISTSSAELRDGDRVKESEKS"
     gene            925..1635
                     /locus_tag="VF958_10895"
     CDS             925..1635
                     /locus_tag="VF958_10895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013032255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX9149656.1"
                     /translation="MTDIIALDHVEKVYHRDALEIPVLNDVNLRVPQGEYLALMGPSG
                     SGKTTLLNLIAGIDQPTRGRVLVGGVDISTVSQTELAKWRSQTIGFIFQLYNLIPVLT
                     AFENVELPLLLTKLSRAERRKHVETALSIVGLSDRVQHYPRQLSGGQEQRVAIARAIV
                     SDPQILVADEPTGDLDAKSAEEILVLLKRLNEEFGKTIVMVTHDPKAASRAHRMIHLD
                     KGVLESDQLTETGHLRRM"
     gene            1646..2812
                     /locus_tag="VF958_10900"
     CDS             1646..2812
                     /locus_tag="VF958_10900"
                     /inference="COORDINATES: protein motif:HMM:NF014717.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HEX9149657.1"
                     /translation="MKYFRLIWRNVFRKKTRTVLTTGSIVLVLVLIVVLVSLLNAMEQ
                     DPSGGRGANRLVVQHATGLASFLPLSQRQRIEQIPGVVAVSPEIWFGGVYKDDRPENF
                     FGQLSADPEIFFDKIFDDATIDPAVNAEWKATRDGFVAGQKLVEKFGWKKGDRIQLRG
                     TYIPITLDLVFIGKYNAGDESNVFFHNEYLNQAKWFGANRVTGIYYMKVASTGDLPRV
                     TAVIDRTFENSDAPTKTMTEKQFQAQFMEMMGNIKGLIRWISLVILFAVTLIVANTVA
                     MSARERVTEISVMRVLGFRRGHVLSFILSESVLMSLLGGIVGVVLAKFVIIPGMVKGM
                     QTTPMAPFLINFKVTWQVLLAAFGISVGVGILAGFVPAIRSSQVRIVDGLRQVV"
     gene            2812..3984
                     /locus_tag="VF958_10905"
     CDS             2812..3984
                     /locus_tag="VF958_10905"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HEX9149658.1"
                     /translation="MAIPLKYNIRNLMVRKVSTGMTVFVICLVVTVFLFVLSLWSGVT
                     RTLSTNASDRNVVTMRVGAQAEMQSVVSRESAELIRAMPGIERDADGQPLVSPELLTL
                     VNITKQDGKSTNVQVRGTSQTGMQLRPSIRIVDGRMFRPGTNEAIVSKVLPTRIANVE
                     IGKTLKSGSYRWQIVGYFDASGSAYDSEIWVDVADLQAQSKRPIYSIVYVRTADRDSA
                     QRYIEAVKGDQRLKFEGKTEKKYFEEQMVTGAPIKILAILVGVFTAIGASFGAMNTMY
                     AQVSARIREIGTLRAIGFSRRSVLASFVLEALLLSLIGGVLGTLLAFVLFNVVITKPT
                     GTMNFRTFSEILFNFRLTPALIVGGLVFSLAMGLFGGFFPAARAARLKITTALREI"
     gene            4032..4760
                     /locus_tag="VF958_10910"
     CDS             4032..4760
                     /locus_tag="VF958_10910"
                     /inference="COORDINATES: protein motif:HMM:NF024118.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEX9149659.1"
                     /translation="MKLRFYGTKGYVEESSPAHAGHSAFTIEADGFRLLCDFGENRKG
                     LLKKIHPDAIFVSHAHPDHCGGLEEGTSAPLYASEITHFLTEKFRVGDRVMLEPGRRV
                     RVGPFRLTAFPVVHSVRCPCIAARLEISGRVLVYSGDIVAFERSEEVLDGAEIYVGDG
                     STLKGSLVRRHSSGTLIGHTTVRAQLGWLAKSGVPRAVFSHFGKGPIEMGEEELREAL
                     EELTSERAPGCQVSAARDGARFDL"
     gene            complement(4812..>6872)
                     /gene="ccsA"
                     /locus_tag="VF958_10915"
     CDS             complement(4812..>6872)
                     /gene="ccsA"
                     /locus_tag="VF958_10915"
                     /inference="COORDINATES: protein motif:HMM:NF027651.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcsA"
                     /protein_id="HEX9149660.1"
                     /translation="GSFLLWCFWGAIIGLFVWRSAKEQEAPVMMVYIATFLGIVAILC
                     KQSPFKLLPPPAPTDGSGLNPLLQDPWMVIHPPVMFLGFASLSVPFAFAIAALWKKRW
                     DGWVPRAIPWALLTFVTLGTAILMGGYWAYKTLGWGGYWGWDPVENTSLVPWLFTTAL
                     VHGMYLQRSKKRHRKVNLILACAAFSTILYGTFLTRSGVLADFSVHSFIDLGITAWLV
                     GILGFFVLLSVALLAVRWRRVPVLEEEETGLLSRGVLFILGIAVFCALACVILLGTSA
                     PLLTRLSGNPSQVQTSFYGKTAAPAALLLAILSGLVPFVSWKSAPTRELLRHLRRSLA
                     VGVAAIVLSLLLGGRDPASLALLFVAATMADMNLRAVIRKVRNGKLLGAGGYLAHVGV
                     GIMLAGIVVSGVYAKTQKLTLVKDEPTKVGDKTLTFLRVVPGSATRKQAMEVRVETAD
                     GKAGYVYPKMYVNSRTGQLMANPAIRRSAVLDYYLSPQQYDPGQPERVGRDVQLTKGT
                     TTNIEGTGFTFRDFNADRSSMMTGGKEVLVLTDLIITPPDGSKHDATVRYVYHLDGAE
                     PEAPEIEIPGHPGAFMRVMAVSPSDGAVVLRLRGMSKDPAADVQAATRESLSVEVTRK
                     PLINLVWGGFYVMMAGGLLALVKRTREARRATVPAIEQSREPAMPAPTALPVTARTTL
                     PTGA"
BASE COUNT         1200 a         2206 c         2317 g         1149 t
ORIGIN      
        1 cggagcgcaa ggcagacctg tctccgaaga taacgtcgcg aatcacggag ctgaacgtga
       61 cggaaggaac ccgcgtcagg aaaggggaca tcgtcgcccg gctcgaccac accgacctcg
      121 acgcccagct cgcggagaag cgcgcggcgt gggtgaacgc gaaggcggag ctcgcgcgcc
      181 agcggtctct gcacgacgaa gggctcgccc cgaagtcgac cctcgacgcc gccgtcgcga
      241 cggaggcgac gacccgcgca gccgtccgct acgtcgaggc gctgctcgac tacacggtca
      301 tccgcgcgcc cttcgcaggc gtcgtgaccg cgaagcgggc gttcgtgggg gaggcggtct
      361 cgccgttcgg ctcgtcgccg tccggcgggg gatccggcgg cgcgatcgtg acgctcgtcg
      421 agttctcgag cctctacgtc ggcgcggacg tcaacgaggc gaacctctcg aagctcggcc
      481 cgaatcagcc ggccgagatc acgctcgacg ccgtcccgga ccggacgtac cacgggtctc
      541 tccgccagat cgtcccggcc gccgaccgcc agaaggcgac ggtgcgcgtg aaggtcgcgt
      601 ttctggacgc cgacgacaag atcctcccgg acctctcggc gcgggtctcg ttcacgagcc
      661 agccaacgca ggggaagacg ggccgtaccc gcgtggtggt tccggcgtcg gcgatccact
      721 cggtcgatgg aaagacgggc gtcttccgga tcgtggacgg gcacgcgcgg ttccaggcgg
      781 tcacggccgg gccggaagcg gacggacagg tggagatccg gcagggcctg gccggagggg
      841 agcgcctcat ttcgacgtcc tccgccgagc tccgcgacgg cgatcgcgtc aaggagagcg
      901 aaaagtcgtg aagggaaggg agagatgacc gacatcatcg cgctcgacca cgtcgagaag
      961 gtctatcacc gggacgcgct cgagatcccg gtcttgaacg acgtcaatct gagggtgcct
     1021 cagggtgagt acctcgcgct catgggaccg tcgggttcgg gcaagacgac gcttctcaac
     1081 ctgattgcgg ggatcgacca gcccacgcga gggcgcgttc tcgtcggcgg cgtggacatt
     1141 tcgacggtct cgcagacgga gctcgcgaag tggcgttcgc agacgatcgg cttcatcttt
     1201 cagctgtaca acctgatccc cgtcctcacg gcgttcgaga acgtcgagct gccgctcctc
     1261 ctcacgaagc tctcgcgggc cgagcggcgg aaacacgtgg agacggcgct ctcgatcgtc
     1321 ggactgagcg accgggttca gcactacccc cggcagctct cgggaggtca ggagcaacgg
     1381 gtcgcgatcg cacgggcaat cgtctccgac ccgcagatcc tcgttgccga cgagccgacc
     1441 ggtgacctgg atgcgaagtc ggccgaagaa atcctcgtcc tcttgaagcg cctgaacgaa
     1501 gagttcggca agacgatcgt catggtgacg cacgacccca aggccgcgtc tcgcgctcac
     1561 cgcatgatcc acctcgacaa gggtgtcctc gagtccgatc agctgaccga gaccggacac
     1621 ctccggcgga tgtgaagacg cgccgatgaa gtatttccgt ctgatctggc gaaacgtctt
     1681 ccgcaagaag acccggacgg tcctgacgac cgggtcgatc gtgctcgttc tcgtcctcat
     1741 cgtcgttctc gtctcgctcc tgaacgccat ggagcaagac ccctccggcg ggcgcggcgc
     1801 gaaccgcctc gtcgtccagc acgccacggg tctggcctcg ttccttccgc tctcccaaag
     1861 acagcggatc gagcagatcc cgggcgtcgt tgcggtatcg ccggagatct ggttcggcgg
     1921 ggtctacaag gacgatcggc cggagaactt cttcggccag ctctcggcgg acccggagat
     1981 cttcttcgac aagatcttcg acgacgcgac gatcgatccg gcggtgaacg ccgagtggaa
     2041 ggcgacgcgc gacgggttcg tcgccggtca gaagctcgtc gagaagttcg gttggaagaa
     2101 gggggatcgg atccagctcc gcgggaccta cataccgatc actctcgacc tcgtcttcat
     2161 cggcaagtac aacgcgggcg acgagtccaa cgtctttttt cacaacgagt atctgaacca
     2221 ggcgaagtgg ttcggcgcga accgtgtgac cgggatctac tacatgaagg tcgcgtcgac
     2281 cggcgacctt ccgcgcgtaa cggcagtgat cgaccggacg ttcgagaaca gcgacgcgcc
     2341 gaccaagacg atgaccgaga agcagttcca ggcgcaattc atggagatga tggggaacat
     2401 caagggcctg atccgctgga tttccctcgt gatcctcttc gccgtgaccc tgatcgtcgc
     2461 caacaccgtc gcgatgtccg cccgcgagcg cgtcaccgag atctcggtca tgcgggtgct
     2521 cggttttcga cgggggcacg tgctgtcctt catcctttcg gagtcggtcc tcatgtcgct
     2581 tttgggaggg atcgtcgggg tcgtcctcgc gaagttcgtc atcatccccg gcatggtgaa
     2641 aggaatgcag acgacgccga tggccccctt cctcatcaac ttcaaggtga cctggcaggt
     2701 gctgctggcc gcgttcggga tttccgtcgg cgtcgggatc ctggccggct tcgttcccgc
     2761 tatccggtcc tcacaggtgc ggatcgtgga cggtctgagg caggtggtct gatggcgatc
     2821 ccgctcaagt acaacatccg gaacctgatg gtgcgaaagg tctcgaccgg catgacggtc
     2881 ttcgtcatct gcctcgtcgt gaccgtgttt ctcttcgtcc tgtcgctctg gagcggcgtc
     2941 acgcggacgc tctcgacgaa cgcttcggac cgcaacgtcg tcacgatgcg tgtcggcgcg
     3001 caggcggaga tgcagagcgt ggtctcgcgc gagtccgccg agctcattcg ggcgatgccc
     3061 ggcatcgaga gggatgcgga cggccagccc ctcgtttctc cggagctcct cacgctcgtc
     3121 aacatcacga agcaggatgg aaagagcacg aacgtccagg tgcgcggtac gtcgcagacc
     3181 ggaatgcagc tgcgaccgtc catccggatc gtcgatggac ggatgttccg tcctggaacg
     3241 aacgaggcga tcgtctcgaa ggtcctcccg acccggatcg cgaacgtcga gatcggcaag
     3301 acgctgaagt cgggcagcta ccgctggcag atcgtgggtt atttcgacgc ttccggctcc
     3361 gcgtacgact cggagatctg ggtggacgtc gccgaccttc aggctcagag caaacggccc
     3421 atctattcga tcgtctacgt gcggactgcc gatcgagact ccgcgcagcg ctacatcgaa
     3481 gccgtcaagg gggaccagcg tctgaagttc gagggcaaga ccgaaaagaa gtacttcgag
     3541 gagcagatgg tgaccggggc gccgatcaag atcctcgcga tcctggtcgg ggttttcacc
     3601 gccatcggcg cctctttcgg cgcgatgaac acgatgtacg ctcaggtctc cgcgcgcatc
     3661 cgggagatcg gcacgctgcg cgcgatcggt ttttcacggc gctccgtgct cgcctcgttc
     3721 gttctcgagg cgttgctcct ctccctgatc ggcggagtcc tggggacgct cctcgcgttc
     3781 gtgctcttca acgtggtgat caccaagccc accggcacga tgaacttccg gacgttttcc
     3841 gagatccttt tcaacttccg cctgacacca gctctcatcg tcggcggtct cgttttctcc
     3901 ctcgcgatgg ggctcttcgg aggctttttc cccgcggccc gcgctgcgag gctgaagatc
     3961 acgacggcgt tgcgggagat ctgatgacaa ccctctccct gcgcgggggg gagggggagt
     4021 aggttcgcgc cgtgaagctc cgcttctacg gcacgaaggg atacgtcgag gagtcctcgc
     4081 cggcgcacgc cgggcattcc gccttcacga tcgaggcgga cggctttcgg ctcctgtgcg
     4141 acttcggcga aaaccggaaa ggtctcctga agaagatcca tcccgacgcg atcttcgtct
     4201 cccacgcgca ccccgaccat tgcggggggc tcgaagaggg cacgtccgcg cccctctacg
     4261 cgtcggagat cacgcacttc ctcacggaga agttccgggt cggggaccga gtcatgctgg
     4321 agccgggtcg tcgcgtccgc gtgggcccgt tccgactgac ggcattcccc gtcgtccact
     4381 ccgttcgctg cccgtgcatc gcggcccggc tggagatctc cggccgggtg ctcgtctatt
     4441 ccggagacat cgtggcgttc gagcgctccg aagaggttct cgacggcgcc gaaatctacg
     4501 tcggcgacgg ttccacgctg aaaggctcac tcgtccgccg tcattcgagc ggcaccttga
     4561 tcgggcatac gacggtgcgg gcgcagctcg ggtggctggc gaagagcggg gtgccgcgcg
     4621 cggtcttctc acacttcggc aagggtccga tcgagatggg ggaggaggag ctccgggagg
     4681 cgctcgaaga gctgacctcc gaacgcgccc ccgggtgcca ggtcagcgct gcgcgggacg
     4741 gggcgaggtt cgacctctag aacatcgaac ctcgtccgtc agcggggcgc tgtggtgctt
     4801 cggtctctcg ctcaggcgcc cgtcggcaaa gtggtcctcg ccgtgacggg aagggcggtc
     4861 ggggcaggca tcgccggctc gcgcgactgt tcgatcgccg ggaccgtggc ccgtcgggct
     4921 tcgcgcgtgc gcttgacgag cgcgaggagg ccgcccgcca tcatgacgta gaagccgccc
     4981 cacacgaggt tgatgagcgg cttgcgcgtg acctcgaccg acagcgactc gcgcgtggcc
     5041 gcctggacgt cggcggcggg gtccttcgac atgccgcgca gccgcaggac gacggcgccg
     5101 tcggaggggg agaccgccat gacccgcatg aatgctccgg ggtggccggg gatctcgatc
     5161 tcgggcgcct cgggctcggc gccgtccagg tggtacacgt agcgcaccgt cgcgtcgtgc
     5221 ttcgatccgt ccggaggcgt gatgatcaga tcggtgagca cgaggacttc ttttccaccc
     5281 gtcatcatcg aggaacggtc ggcgttgaag tcacggaacg tgaagccggt gccttcgatg
     5341 ttggtcgtgg tccccttcgt cagctgcacg tcgcggccca cgcgctcggg ctggccgggg
     5401 tcgtactgct gcggggagag gtagtagtcg agcacggccg accggcggat cgcgggattc
     5461 gccatcagct ggccggtgcg cgagttcacg tacatcttgg gatacacgta tccggccttt
     5521 ccatcggcgg tctcgacgcg aacctccatg gcctgcttcc gtgtggcgga gcccggcacg
     5581 acgcgcagga acgtgagcgt cttgtcgccg accttcgtcg gctcgtcctt gacgagcgtg
     5641 agcttctggg tcttcgcgta gaccccggag acgacgatgc ccgcgagcat gatgccgacg
     5701 cccacgtggg ccaggtagcc gccggcgccg aggagcttgc cgttgcgcac cttgcgaatc
     5761 acggcgcgca aattcatgtc ggccatggtc gccgcgacga acagcagcgc aaggctcgcc
     5821 gggtcgcgtc cgccgagaag cagcgaaagg acgattgctg cgacgccgac cgcgagagac
     5881 cggcgcaggt ggcgcagaag ctcccgggtc ggggcgctct tccaggacac gaaggggacg
     5941 agccccgaga gaatggcgag gagcagagcg gccggggccg cggtcttgcc gtagaaggac
     6001 gtctgcacct gggaggggtt tccggagagc cgggtcagga ggggcgcgga cgtgccgagc
     6061 aggatgacgc acgccagcgc gcaaaagacg gcgatgccca ggatgaacag cacgccgcgc
     6121 gagagcaggc ccgtctcttc ctcctcgagc accggaaccc ggcgccaccg caccgcgagg
     6181 agcgccaccg acagcagaac gaagaacccg agaatcccca cgagccatgc cgtgatgccc
     6241 agatcgatga aggagtggac cgagaagtcc gcgaggacgc cggaacgcgt gaggaacgtc
     6301 ccgtagagga tggtcgagaa cgcggcgcac gcgaggatca ggttgacctt gcgatgccgc
     6361 ttcttggatc gctgcaggta catgccgtgg acgagcgcgg tggtaaagag ccagggcacc
     6421 aggctcgtgt tctcgaccgg gtcccaaccc cagtagcctc cccagccgag cgtcttgtag
     6481 gcccagtagc cccccatgag gatcgccgtg ccgagcgtga cgaacgtcag cagagcccag
     6541 ggaatggcgc gagggaccca tccgtcccac cgcttcttcc acagcgccgc gatcgcgaag
     6601 gcgaacggga ccgagagcga cgcgaaaccg aggaacatga cgggcggatg gatgaccatc
     6661 caggggtcct gcagcagcgg attcagcccc gagccgtccg tgggggcggg gggcggcagc
     6721 agcttgaacg ggctctgctt gcagaggatc gcgacgatgc cgaggaacgt cgcgatgtaa
     6781 accatcatga ccggcgcttc ctgctccttc gcgctgcgcc agacgaagag cccgatgatc
     6841 gctccccaga agcaccagag caggaacgag cc
//
LOCUS       DASWAV010000521         3378 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U2665
            k141_788472, whole genome shotgun sequence.
ACCESSION   DASWAV010000521 DASWAV010000000
VERSION     DASWAV010000521.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960316
            Sequence Read Archive: SRR10260007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 3378)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3378)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:47:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,929
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3378
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_788472"
                     /isolate="SMAG_U2665"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.36444 N 132.31889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..948)
                     /locus_tag="VF958_10920"
     CDS             complement(<1..948)
                     /locus_tag="VF958_10920"
                     /EC_number="1.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014818.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004365 - glyceraldehyde-3-phosphate
                     dehydrogenase (NAD+) (phosphorylating) activity [Evidence
                     IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HEX9149661.1"
                     /translation="MTKVGIFGFGRIGRNLFRLLYEREDIRIAAISDWNDPEPLEYLL
                     EFDTLLGRFPSEVSIREGHLYAAGRQIRMITGKDQPQPPPWGELGVDTVLEATSRGRT
                     RAELEAHLAAGARRVIACAPPLEPPDATIVMGVNEAALERRHRIVSNASSTVHCLAPV
                     LKILDDAFGIERALFTTVHSYTSQHRLADVPADEMRRGRAAAENIIPQESRSPGMLME
                     LLPQLRAKITGASVAVPVRNGSCVDLVTWHSHPVTKTAVNEVLRTAASTDRWQHVLRY
                     ESDPIVSSDVARSWWSSTFDSLATMTLGDRVSKTISWYDS"
     gene            complement(945..1766)
                     /locus_tag="VF958_10925"
     CDS             complement(945..1766)
                     /locus_tag="VF958_10925"
                     /inference="COORDINATES: protein motif:HMM:NF023568.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX9149662.1"
                     /translation="MARSLLPVLGALLLAWIALLIAMSAMEQRFLYFPVRTLEAQPSD
                     YGLRSEELRVTTSDGVRLHGWWIRAGGSFVVLLFHGNAGNISHRLDRAKLFAERLGVD
                     LFLVDYRGYGRSGGKPSEEGLYFDGLAIYDAARERGFPSNRIVLLGESLGCAVAAEVA
                     LARPVAAVALETPFLSVPALARKHYPFVPTFLVRSKFDTEATIGRLTVPKLIVAAERD
                     EIAPPSHARRLFELALPPKELSVIPGAGHNDTYVAGGEPYLEVWRRFLNAIDSTP"
     gene            complement(1778..3268)
                     /locus_tag="VF958_10930"
     CDS             complement(1778..3268)
                     /locus_tag="VF958_10930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9149663.1"
                     /translation="MNERRARWIALALLGVAALVAVVALVLPNVLLRSDFLRKQINTD
                     PDSGWLEYDAASSRWPGTLHVKNFRFRDRDPKAEWAMSLDEADVTYSLGDLLRRRFHV
                     TSLSGRGGVFRVRSRLTPAEAKSPTLLSRLPPIAGFSDPPLLGPPERKPPRSGKVWTI
                     QIDRLAIDDLREVWVEGYRCTGESRLTGGFFLRPEQRAEIFPSKLSIRRGTIRSGKDV
                     IAADVSATFDARFDPWNPRELPGSKVLAVLFGGAQATARLDDAEIVNRLLGEPAGTRF
                     ERGSGRMTVRASVERGVVAGSIAYEARDLTLRALDVAMRGRFDGRLELSDIRMENWGG
                     GRLNGGHVSLTDGTALDRDGSRYPWWARVDFSSGEFRPKTAALFTTKATARARNAQPL
                     LQIAGVDLPGWADRFLRLDEAMTGRADVRVGNSLLELKRLAARTGKVEIFGDYAARNR
                     SRNGTFLVDAGLLSVGIGTGTGEKQVRIFGPRKWFRERTGWEPEKD"
BASE COUNT          570 a         1094 c         1199 g          515 t
ORIGIN      
        1 cgagtcgtac caggaaatcg tcttcgagac cctgtcgccg agggtcatgg tcgccagcga
       61 atcgaaggtg ctcgaccacc acgagcgggc gacgtcggag gacacgatcg ggtcggactc
      121 gtaacggaga acgtgctgcc agcggtcggt cgaagcggcg gtcctcagga cctcgttcac
      181 agccgtcttc gtgactggat gcgagtgcca cgtgacgagg tcgacgcaag agccgtttcg
      241 cacgggaacg gcgaccgagg cgccggtgat ctttgcgcgg agctgcggca ggagctccat
      301 cagcatcccg ggagagcgcg attcctgcgg gatgatgttc tctgcggccg cgcgcccccg
      361 ccgcatctcg tcagccggga cgtcggcgag ccggtgctgg ctcgtgtagg agtgcacggt
      421 cgtgaagagg gcgcgctcga ttccgaacgc gtcgtcgagg atcttcagga cgggcgcgag
      481 gcagtggacc gtcgaagatg cgttcgagac gatccgatgc cgccgttcca gggcggcctc
      541 gttcaccccc atcacgatgg tggcgtcggg cggttcgagc ggcggggcgc aggcgatgac
      601 gcgccgcgcg cccgcggcga ggtgcgcttc gagctccgcg cgggtccggc cgcgcgaagt
      661 cgcctcgagg acggtgtcga ccccgagctc gccccagggc ggcggctgcg gctggtcctt
      721 gccggtgatc attcggatct gccgcccggc ggcgtagaga tgtccttccc ggatcgagac
      781 ttcggagggg aagcggccga gcagcgtgtc gaactccagg aggtactcga gcggctccgg
      841 gtcgttccag tcgctgatgg cggcgatgcg gatgtcctcg cgctcgtaga gcaaccggaa
      901 gaggttccga ccgattcgcc cgaacccgaa gatcccgact ttcgtcacgg ggtggaatct
      961 atcgcgttca ggaaacgacg ccagacctcg agatacggtt cgccccccgc gacataggtg
     1021 tcgttgtggc cggcgcccgg aatcacggag agctccttcg gcggcaaagc gagctcgaag
     1081 agccgccggg cgtgggacgg aggagcgatc tcgtcgcgct cggccgcgac tatcagcttt
     1141 ggaacggtca gccggccgat cgtcgcttcg gtgtcgaatt tcgaacggac gagaaaggtc
     1201 ggtacgaacg ggtagtgctt gcgcgcgagg gcggggaccg agagaaaggg cgtctcgagg
     1261 gcaactgctg cgaccgggcg agcgagcgcg acctcggcgg cgacggcgca gccgagcgat
     1321 tcgccgagca ggacgatgcg gttcgatgga aaaccgcgct cgcgcgccgc gtcgtagatc
     1381 gccagcccgt cgaagtagag gccttcttcg gaaggttttc cgccggagcg gccgtagccc
     1441 cggtagtcga cgagaaagag gtcgacgccg aggcgctccg cgaagagctt cgctcgatca
     1501 agccggtgcg agatgttgcc tgcgtttccg tgaaaaagga gcacgacgaa ggatccgccc
     1561 gcgcggatcc accagccgtg gagacgaacg ccgtccgagg tcgtcacgcg aagttcctcg
     1621 ctgcgcaggc cgtagtcgga gggttgcgct tcgagggtcc ggacggggaa atagaggaac
     1681 cgttgctcca tcgcgctcat ggcgatgagc agggcgatcc aggccagcaa gagcgcgccg
     1741 agcacgggaa gcaacgacct cgccacgaca tccgaggtta gtccttctcc ggctcccatc
     1801 cggttcgttc ccggaaccac ttgcgcgggc cgaagatgcg cacctgtttt tccccggttc
     1861 cggttccgat gccgaccgac agcaatcccg cgtcgaccag gaacgttccg tttctcgagc
     1921 gattccgcgc ggcgtaatcg ccgaagatct cgacctttcc ggtgcgggcc gccaggcgtt
     1981 tgagctcgag gagagaattt cccacacgga cgtctgcgcg cccggtcatc gcctcgtcga
     2041 gccgaaggaa acggtccgcc cagccgggca gatcgacccc ggcgatctgg agcagcggct
     2101 gggcgtttct cgcgcgcgcg gtcgccttcg tcgtgaagag ggccgccgtc ttcggacgaa
     2161 actcgcccga gctgaagtcc acgcgggccc accacgggta acggctgccg tcgcgatcca
     2221 gagcggtccc gtcggtcaaa gagacgtggc cgccgttcag gcgtcccccg ccccagttct
     2281 ccatccggat gtcggacagc tcgagccgcc cgtcgaaccg cccgcgcatg gccacgtcga
     2341 gcgcgcgcag cgtgaggtcg cgcgcctcgt aggcgatcga ccccgccacg acgccgcgtt
     2401 cgaccgaggc gcgcacggtc atgcggccgc ttccccgttc gaaccgggtt ccggcgggct
     2461 caccgaggag gcggttcacg atctccgcgt cgtccaggcg agcggtcgcc tgcgccccgc
     2521 cgaagaggac agcgagcacc ttgctccccg gaagctcgcg cggattccag ggatcgaacc
     2581 gcgcgtcgaa ggtcgccgag acatccgccg cgatgacgtc cttccccgat cggatcgttc
     2641 cccggcggat gctgagcttc gagggaaaaa tctccgcgcg ctgctccggc cggagaaaga
     2701 atccgcccgt caggcgcgac tcgccggtgc accggtatcc ctcgacccac acctcgcgca
     2761 ggtcgtcgat cgccaaccgg tcgatctgga tcgtccagac cttgccggac cgcggcggct
     2821 tccgctccgg gggtccgagg agcggcggat cggaaaagcc cgcgatcggc ggcagacggg
     2881 agaggagagt gggcgatttc gcttccgcgg gagtgaggcg gctccggacg cggaagacgc
     2941 ctccccgccc cgacaggctc gtgacgtgga aacgtcgccg gaggaggtcg ccgagagagt
     3001 acgtgacgtc cgcctcgtcg agcgacattg cccactcggc cttcgggtcg cgatcgcgga
     3061 atcgaaaatt cttgacgtga agagttccgg gccagcggga agaggcggca tcgtattcga
     3121 gccagccgct gtccggatcc gtattgattt gcttgcggag gaagtcggag cggaggagaa
     3181 cgttcgggag gaccagcgcg acgaccgcga cgagagcggc gacaccgagg agagcaaggg
     3241 cgatccatcg agcccggcgt tcgttcaccc ggggcctctc cgcaagggcc ctgccaacag
     3301 gtgtgaggcg cggaacggga ctccgtcagc ggcgcttcgg cggtcgggcc cggaccggcg
     3361 acttcgcggc gggcttcg
//
LOCUS       DASWAV010000522         6703 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U2665
            k141_229353, whole genome shotgun sequence.
ACCESSION   DASWAV010000522 DASWAV010000000
VERSION     DASWAV010000522.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960316
            Sequence Read Archive: SRR10260007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 6703)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6703)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:47:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,929
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6703
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_229353"
                     /isolate="SMAG_U2665"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.36444 N 132.31889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..980)
                     /locus_tag="VF958_10935"
     CDS             complement(<1..980)
                     /locus_tag="VF958_10935"
                     /inference="COORDINATES: protein motif:HMM:NF013871.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9149664.1"
                     /translation="MSPARDLARERGIALYLVGGAVRDLLLGRPRRIVDVDLVVESDA
                     AGFARELARRTGSAVKLHGRFGTAVLEGPSGDRLDVATARAEDYEQPGALPRVRSGSI
                     DDDLARRDFTVNAMAIEIARAGRPRLLDPLGGREDLSRGLVRMLHPRSPFDDPTRAFR
                     AVRYANRLGFRIERGTRRWIREAVGKGVLEAVSGDRVRREIALLFSERGRAAAARHLA
                     ALGIAAAVHPSLRYDAAAARRLRAAERLATASGLDTTWVVYLLSWMGEIGERAAEGIA
                     ARLSLPRAAAAAVRGWPRTWRRLAAAAGGGHSHRLAAVEAVSDEELLAAAA"
     gene            complement(1018..4500)
                     /gene="dnaE"
                     /locus_tag="VF958_10940"
     CDS             complement(1018..4500)
                     /gene="dnaE"
                     /locus_tag="VF958_10940"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF005298.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HEX9149665.1"
                     /translation="MPADFVHLHLHTQYSLLDGANRVGEMCRRIRELEMPAVAVTDHG
                     NMFGAFSFYHEALKSGVRPILGVEAYLAPGDRRDREAQAAVESGEGYAYHLTILAATQ
                     AGYQNLIKLVSEAYLTGFYHRPRMDKALLREHAHGLIALSGCLKGEVAGALSRGNVEA
                     ARRAVHEYQDIFGKENFFIELMDHGLPKQTEILPELLRLSTETGAPVVATNDSHYLHR
                     EDAFAHEVLLCIGMGKTLEDERRMRFYNDEFYVKSAQEMRDRFRRWSEESLSNTVAVA
                     ERCAVTFDTEGLHLPTFTAPEGKTPAEHFTELAREGLERRLAELEPFFRAGKVPNPPE
                     KYRERLEYEIGVVEKMGFPSYFLIVSDFIRHARESGISVGPGRGSAAGSIVAWALRIT
                     EIDPLRYDLLFERFLNPERISMPDIDIDFCQARRGEVIEYVTKKYGRENVAQIVTFSQ
                     LKPKLAVRDVARVLSLPVAIGDRIAKLIPDGVDVNFERALKESPLLKEAIATDESVAR
                     VVQIAGRLEGLSRHAGMHAAGVVIAPRPITEYLPLYRTNTGDVTTQFDMNAVEKMGLL
                     KIDFLGLITLDIMDAAVHAIRDRTGDAVDLEHLPLNDEKTYELFRSGKTACVFQFDSS
                     GMRDLLRRAKPKVFSDLAALNALYRPGALDAGTVEEYVRRRNGTSKVSYPLPEIEDIL
                     EETFGILVYQEQVMRIASRVAGYSLAEADLLRKAIGKKKREIMVAEGEKFIRRSEEHG
                     TPKKKAQQLWALIEPFARYGFNKSHAVAYALVAYKTAYLKAHYPIDFLAAALSAEIGS
                     TDGIVKVVGDCTEMGIPVLPPDINESRKNFAAVGPAIRFGLAAIKGVGDQAADSILQE
                     RENRPFTSFTDLAHRLDPHLVNKRTLDALICAGALDSLGKNRATLASASERVVAQAAR
                     RREEAELGQTNLFAGGSDGDATDVFREEPEWTLEEKLTKEKEVLGFYVTGHPLTKYAE
                     DIDRFADARVQELVSKVDQTVRVAGVLVNVKKQKIKKGVNEGKTMLKATLEDTTGGVP
                     VCVFASLYEKVASWIRADLPVLMTAIAREAGGSIELTVQDVTPLEGIRERLAREMEIR
                     IDLTLADETLMERLKELLRLNSGTTPLALRLVRPGDFDVKLKAADSIRVTPSPRLTGQ
                     VRALTGEDSVRFNF"
     gene            4563..5894
                     /locus_tag="VF958_10945"
     CDS             4563..5894
                     /locus_tag="VF958_10945"
                     /inference="COORDINATES: protein motif:HMM:NF024910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HEX9149666.1"
                     /translation="MSFRRALGPFDATMVVVGGIVGAGIFINPYLVAGRLSSGGLVLA
                     AWIAGGVIALAGAFAFAELAALFPRAGGQYVYLREAYHPIVAFLFGWASLLMIQGGGL
                     AAVAITFAQYTLRLTGAREAAVVPLAIIAIALVAAVNVLGVKPGSRLLNVLVLLKIGA
                     LTVLILGGLFLAHPATGPPPSTGVPAGGAGPLAFGAALIPILFSYGGWQGASVVAEEM
                     RDPRRTLPLALLWGTALVIAIYVLANVVYLLALGRDGLAATPTPAAETVRRIFGPGAD
                     RLIAATIAVSAFGFLDLTLLAYTRIYWAMAKDGLFFERLARLHPRFQTPALAIVLQGA
                     WGIVLVATGSYGELVDSVVFADWIFFGLAGAAIFLFRRRIPLEAREPDSFRTPGYPIA
                     PALFVAAALYVVASALLSNPGRALVGSAILALGAPMFLFFSRRRQRAGNPA"
     gene            5894..>6703
                     /locus_tag="VF958_10950"
     CDS             5894..>6703
                     /locus_tag="VF958_10950"
                     /inference="COORDINATES: protein motif:HMM:NF012382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HEX9149667.1"
                     /translation="MVRAASPYMEWAKARPAPEIDLATSNLLACTLDDLPGAREAVEL
                     AGASPNGYRPLVEAIAAQYGVLPENVATAVGCSGANFLALAALVEAGDDVLFEQPGYD
                     PLAAAATMLGARVARFERRFENGWRVDPERIACALTPRTRVVMLSSPHNPSGVLAGAD
                     DLLGIGRLAERHNFTVIVDEVYLDLASSAHPPAATLSPFFVSTNSLTKAYGLASLRCG
                     WALASPGIAERIRRARDVVDVWSPMPSDRLSVIAFRRLDRLLNRARAIVATN"
BASE COUNT          962 a         2352 c         2228 g         1161 t
ORIGIN      
        1 gccgcagcgg cgagcagctc ttcgtcggag acggcttcca ccgcggcgag ccggtgcgag
       61 tggccgcctc ccgccgcagc cgcaagtcgt ctccacgtcc tcggccagcc tcggacggca
      121 gctgcagccg ctcgcggcag gctcagccgt gcggcgattc cctccgccgc tcgctcccct
      181 atctcgccca tccacgacag aaggtacacg acccaggtcg tgtcgaggcc ggatgccgtc
      241 gcgagacgct cggcggcgcg caggcgacgc gccgcggcgg cgtcgtatcg aaggctcgga
      301 tggaccgccg ccgcgatgcc gagagcggcg agatgccgcg ccgcggccgc ccgtcccctc
      361 tccgagaaca gaagggcaat ctcccggcgc acccggtcgc ccgaaaccgc ctcgagaacc
      421 cccttcccga ccgcctcgcg gatccaccgg cgcgtgccgc gctcgatccg gaagcccagc
      481 cggttcgcgt agcggactgc gcggaatgcc cgggtggggt cgtcgaaggg cgaccgcggg
      541 tgcagcattc gaacgaggcc gcgagagaga tcctcgcggc ccccgagcgg atcgaggaga
      601 cggggccgcc cggcacgcgc gatttcgatc gccatcgcat tgaccgtgaa gtcgcgacgg
      661 gcgaggtcgt cgtcgatcga gccggaccgg acgcggggaa gcgcgcccgg ctgctcgtag
      721 tcctcggccc gcgcggtcgc gacgtcgaga cggtctcccg agggcccctc gaggacggcg
      781 gtgccgaagc gcccgtgcaa cttcaccgcg gagccggtcc gccgggcgag ctcgcgggcg
      841 aaaccggccg cgtccgactc gacgacgaga tcgacgtcga cgattcgccg gggccggccg
      901 agcagcaggt cgcgcaccgc gccgccgacg aggtagagcg cgatccctcg ctcccgcgca
      961 aggtcgcgcg ccggcgacac ccgccgaccg atttccttcg gaagcgcggg cgcctcccta
     1021 gaagttgaag cggaccgagt cctcacctgt gagagcgcgc acctgtcccg tcagccgcgg
     1081 cgagggcgtg acgcggatcg agtccgcggc tttcagcttg acgtcgaagt cccccggccg
     1141 cacgaggcgc agcgcgagcg gcgtcgtgcc cgaattcaac cgcaggagct ccttcaagcg
     1201 ctccatcagg gtctcgtcgg cgagggtgag gtcgatgcgg atctccattt cgcgcgcgag
     1261 gcgctcgcgg atcccctcga gaggcgtcac gtcctgcacc gtcagctcga tcgaccctcc
     1321 cgcctcccgc gcgatcgcgg tcatgagtac cgggagatcc gcgcggatcc aggaggcgac
     1381 cttctcgtag agactcgcga agacgcacac gggaacgcct cccgtggtgt cctcgagggt
     1441 cgccttcaac atcgtctttc cttcgttgac gcccttcttg atcttctgct tcttcacgtt
     1501 gacgaggact cccgcgaccc ggacggtctg atcgaccttc gagaccagct cctgcacgcg
     1561 cgcgtccgcg aaacgatcga tgtcctcagc gtatttcgtg agcggatgcc cggtgacgta
     1621 gaagccgaga acctccttct ccttcgtgag tttctcctcg agcgtccact ccggctcctc
     1681 ccggaagacg tccgtcgcgt cgccgtcgga tcctccggcg aaaaggttcg tctgccccag
     1741 ctccgcctcc tcccggcggc gcgccgcctg cgcgaccacc cgctcggacg cggaggcgag
     1801 cgtcgcgcga ttcttcccga gagaatcgag ggcgcccgcg cagatgagag cgtcgagcgt
     1861 gcgcttgttg acgaggtgcg ggtcgagtcg gtgcgcgagg tcggtgaacg aggtgaacgg
     1921 ccggttctcg cgctcctgca ggatcgagtc ggccgcctga tccccgacgc ccttgatggc
     1981 ggcgaggccg aaccggatcg ctggaccgac cgccgcgaag ttcttccggg actcgttgat
     2041 gtcgggaggg agcaccggaa tgcccatttc ggtgcagtcg ccgacgacct tcacgattcc
     2101 gtcggtcgaa ccgatctccg cagagagtgc cgcggcgagg aagtcgatcg ggtagtgggc
     2161 cttcaggtac gccgtcttgt aggcgacgag cgcgtacgcc accgcgtgcg acttgttgaa
     2221 cccgtacctt gcgaagggtt cgatcagggc ccagagctgc tgcgccttct tcttcggggt
     2281 gccatgctcc tccgaccgcc ggatgaactt ctctccctcg gcgaccatga tctcgcgctt
     2341 cttcttgccg atcgccttcc gcagcagatc cgcctctgcg agcgagtacc ccgccacgcg
     2401 cgaagcgatc cgcatgacct gttcctggta cacgagaatc ccgaacgtct cctcgagaat
     2461 gtcctcgatc tcggggagcg ggtagctcac cttgctcgtg ccgttcctcc gacgcacgta
     2521 ttcctccacg gtgccagcgt cgagcgcgcc cggacggtag agcgcgttca gcgccgcgag
     2581 gtccgagaag accttcggtt tcgcgcgccg caggaggtcc cgcattccgg acgagtcgaa
     2641 ctggaacacg caggccgtct tccccgagcg aaagagctcg tacgtctttt cgtcgttcaa
     2701 cggcaggtgc tcgaggtcga ccgcatcgcc ggtgcgatcg cggatcgcgt gcaccgccgc
     2761 gtccatgatg tcgagcgtga tcagtcccag gaaatcgatc ttcaagagac ccatcttctc
     2821 gacggcgttc atgtcgaact gcgtcgtcac gtcgcccgtg ttcgtgcgat acagggggag
     2881 atattccgtg atcgggcgcg gcgcgatcac gacgccggcc gcatgcattc ctgcgtgccg
     2941 ggagaggcct tcgagccgtc cggcgatctg gacgaccctg gcgaccgact cgtcggttgc
     3001 tatggcctcc ttgagcagcg gcgattcctt gagcgcgcgc tcgaagttca cgtccacgcc
     3061 gtccgggatg agcttcgcga tgcgatcgcc gatcgccacc ggcagcgaga ggacccgcgc
     3121 gacatcgcga accgcgagct tcggcttgag ctgcgagaac gtgacgatct gcgcgacgtt
     3181 ctcccgcccg tacttcttcg tgacgtactc gatgacctcg ccgcggcgcg cctggcagaa
     3241 gtcgatgtcg atgtcgggca tcgagatccg ctcggggttc aggaacctct cgaagaggag
     3301 gtcgtagcgc agcggatcga tctcggtgat gcgaagagcc cacgccacga tcgaacccgc
     3361 cgcacttccc cgacccggcc cgaccgagat gccgctctcg cgggcgtgcc ggatgaagtc
     3421 ggagacgatc aggaagtagg aggggaatcc catcttctcg acgacgccga tttcgtactc
     3481 caggcgctcg cggtacttct cgggggggtt ggggaccttc ccggcgcgaa agaacggctc
     3541 gagctccgcc aggcgcctct cgagcccctc gcgggcgagc tcggtgaaat gttcggccgg
     3601 agtcttcccc tccggcgcgg tgaacgtcgg aaggtggagg ccctcggtgt cgaacgtgac
     3661 ggcgcagcgt tccgcgaccg ccaccgtgtt ggacagcgac tcctcggacc accgccggaa
     3721 gcggtcgcgc atctcctgcg cgctcttcac gtagaactcg tcgttgtaga acctcatgcg
     3781 gcgctcgtcc tcgagggtct tgcccatgcc gatgcagagc agcacctcgt gagcgaacgc
     3841 gtcctcccgg tgaaggtagt ggctgtcgtt ggtcgcgacg accggtgcgc cggtctcggt
     3901 ggagagccgg aggagctcgg gaaggatctc ggtctgcttc ggcaggccgt ggtccatcag
     3961 ctcgatgaaa aagttctcct tgccgaagat gtcctggtac tcgtggacgg ccctccgggc
     4021 cgcttcgacg ttgccgcgcg aaagcgcacc ggcgacttcg cccttgagac acccggacag
     4081 cgcgatcagc ccgtgggcgt gctcgcgcag gagcgccttg tccatccggg gtcggtggta
     4141 gaagcccgtc aggtacgctt cggaaacgag cttgatgagg ttctgatagc ccgcctgcgt
     4201 cgccgcgagg atcgtcaggt ggtacgcgta gccctctccc gactcgacgg ccgcctgcgc
     4261 ttcccggtcg cggcggtcgc ccggcgcgag gtatgcctcg accccgagga tcggccggac
     4321 gccgctcttc agagcctcgt ggtagaagga aaacgccccg aacatgttcc cgtggtcggt
     4381 gacggcgacc gcgggcatct cgagctcccg gatccggcgg cacatctctc ccacgcggtt
     4441 ggcgccgtcg agaagggagt actgcgtgtg gaggtgaagg tggacgaagt cggccggcat
     4501 ccttcccgat tgtagtgtcc cgggtcccaa gtcggaagtc cctgacgata ttctcaacag
     4561 cgttgagttt ccgccgcgcg ctcgggccct tcgacgcgac aatggtggtc gtcggcggga
     4621 tcgtcggcgc cggaatcttc atcaatccgt acctcgtcgc ggggcggctc tcttccggcg
     4681 gtctcgtgct cgcggcgtgg atcgctggcg gcgtcatcgc actcgccggc gcgttcgcgt
     4741 tcgcggagct ggccgcgctc ttcccccgcg ccggtgggca gtacgtctac cttcgcgagg
     4801 cgtatcaccc gatcgtcgcc ttcctgttcg ggtgggcgtc gctcctgatg atccaggggg
     4861 gcgggctggc cgcggttgcg atcacgttcg cgcagtacac gctgcgcttg accggcgccc
     4921 gagaagcggc cgtggtcccg ctcgcgatca tcgccatcgc tctcgtcgcg gcggtcaacg
     4981 tcctcggcgt aaaacccggg agcaggctcc tgaacgtcct cgtcctgctc aagatcgggg
     5041 cgctcaccgt gctcatcctc gggggactgt ttctcgcgca cccggccacc ggaccgccgc
     5101 cttcaaccgg agtccccgcg ggcggcgcgg ggccgctcgc gttcggggcg gcgttgatcc
     5161 cgatcctctt ctcctacggc ggctggcagg gcgccagcgt cgtggcggaa gagatgcgcg
     5221 atccgaggcg aacgctcccc ctcgccctcc tctgggggac ggccctcgtg atcgcgatct
     5281 atgtgctcgc gaacgtcgtg tatctgcttg cgctcggccg cgatggcctc gccgcgacgc
     5341 ccaccccggc agcggaaacg gtccggcgta tcttcggccc cggcgccgac cggctgattg
     5401 ccgcgacgat cgcggtctcg gccttcggtt tcctcgactt gacgctgctc gcgtacaccc
     5461 ggatctactg ggcgatggcg aaggacgggc tcttcttcga gcgactggcc cgcctgcacc
     5521 cgaggttcca gactcccgct ctcgcgatcg tccttcaggg cgcctgggga atcgtcctcg
     5581 tcgcgacggg gtcctacggt gaactcgtgg attcagtggt gttcgccgac tggatcttct
     5641 tcgggcttgc cggcgccgcg atcttcctct tccggcgacg aatcccgctc gaggcgcggg
     5701 aacccgactc cttccggacc ccgggatatc cgatcgcgcc ggctctcttc gtcgccgccg
     5761 cgctgtacgt cgtcgcgagc gcgcttctct cgaaccccgg gcgcgcgctc gtcggaagcg
     5821 cgattctcgc gctcggggcg ccgatgtttc tcttcttctc gcggcgacgc cagcgagccg
     5881 ggaaccccgc ctgatggtcc gcgccgcctc cccctacatg gagtgggcca aggcacgccc
     5941 ggcgccggag atcgatcttg cgacttccaa cctgctcgcg tgcactctcg acgacctgcc
     6001 gggcgcgcgc gaggccgtcg agctcgcagg cgcgagcccg aacggttacc ggccgctcgt
     6061 cgaggcgatc gccgcacagt acggcgtact cccggagaac gtggcgacgg ccgtcggatg
     6121 ctcgggtgcg aattttctgg cgctcgcggc actcgtcgaa gccggagacg acgtcctgtt
     6181 cgagcagccg ggttacgacc cgctcgcggc cgcggcgacg atgctcggcg cgcgggtcgc
     6241 gcggttcgag cgccggttcg agaacggctg gagggtcgac cccgaacgga tcgcctgcgc
     6301 gctcacgccg cgcacgcggg tcgtcatgct ctcgtcgccg cacaacccct cgggcgtgct
     6361 ggcgggggcg gacgatctcc tcgggatcgg acggctcgcc gagcgccata acttcacggt
     6421 gatcgtcgac gaggtctatc tcgacctcgc gtcgtcggcc catccgcccg cggcgacgct
     6481 ctccccgttt ttcgtctcca ccaacagcct gaccaaagcc tacggtctcg cctcgctgcg
     6541 ctgcggctgg gcgctcgctt ctcccggcat cgccgagagg atccgccgcg cccgcgacgt
     6601 cgtggacgtc tggagcccga tgccgtcgga tcgcctctcg gtgattgcgt tccgacgatt
     6661 ggaccgcctg ttgaatcgcg cgcgagcgat cgtcgccacg aac
//
LOCUS       DASWAV010000523         7676 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U2665
            k141_387116, whole genome shotgun sequence.
ACCESSION   DASWAV010000523 DASWAV010000000
VERSION     DASWAV010000523.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960316
            Sequence Read Archive: SRR10260007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 7676)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7676)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:47:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,929
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7676
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_387116"
                     /isolate="SMAG_U2665"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.36444 N 132.31889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            2..814
                     /locus_tag="VF958_10955"
     CDS             2..814
                     /locus_tag="VF958_10955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014559621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX9149668.1"
                     /translation="MAGDTQTRWALVLGASSGFGEACSIALAKAGWNIFGVHLDRKST
                     QPNVDRIVAEIAGAGRKAEFFNVNAADDGKRTEVVAAISRACADAPPKGRVSVVLHSL
                     AFGTLKPFFGQPRENALSKAQLEMTLDVMGNSIVYWVQDLFFAGLLAPGARIFAMTSA
                     GDHIVWPAYGAVSAAKAVLESHVRQLAVELAPHRIAVNALRAGVTDTPALRKIPGNEQ
                     MIERILAIHPAGRLTTPADVAGALVALCADGAAWVSGNVIGVDGGEDVAGGR"
     gene            865..2166
                     /locus_tag="VF958_10960"
     CDS             865..2166
                     /locus_tag="VF958_10960"
                     /inference="COORDINATES: protein motif:HMM:NF017044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HEX9149669.1"
                     /translation="MAPVPEAFEFVREKAGIREFRLRSNGLRVLLLRNTVAPVATFAV
                     VYHVGSRNEAVGHTGATHLLEHLMFKGTPEFDREKATAVAAVLEALGARFNATTWFDR
                     TNYYETIPSDQIETAIRIEASRMRGAYIRDSDRAPEMTVVRNEYERGENSPFQVLYKE
                     TFATAFREHPYHHPTIGWRADIEGVTTERLKEFYDVFYHPNNATAMLIGDFEEPEALA
                     TLSRHFGRIPPSARPIPPVYTTEPAQEGERRFTVRRTGQVAWVALSWRTVAARHPDTP
                     VLAALGNVLGGGLTARLHLALVETSLALSVTVVPWQLRDPALFSVFAPVRPGVAPEDV
                     EKRIREEITRVALQGVTEAEAEKARTQIEAEVIFERDSTDQVAASLSEAIAVADWEWY
                     ADYPEMVRRVTAEDVRRVAATYFTDDSLTVGAYLPKETPAA"
     gene            2163..3482
                     /locus_tag="VF958_10965"
     CDS             2163..3482
                     /locus_tag="VF958_10965"
                     /inference="COORDINATES: protein motif:HMM:NF017044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HEX9149670.1"
                     /translation="MTISPAATAAVRRGKIPFEDAVRRRTLANGARLFVLENRFNPTV
                     AISGSLNAGPLFAPPDRRLLASMTAGELAKGTERRTKLEIAEELESRGASFSIAAGSS
                     DPVGVDLAAASLSRDVEVLLDVLAEVLLSPVFPAEELEKERMRLVGVLREQQDQTSVR
                     AFEAAARRLYPPGHPFHRRRAEERIAVLESLERAELRRYYDERYGAATFILVVVGDVD
                     SDRLLDGLERRLGSWKPGPAAEIEKPPPVAPSPGAETVVMPDKASADVVLAQPADLDR
                     LDPEYLPCVLANSALGQSSLTSRLGVRVRDTEGLTYGIHSGFSASHLAGPFTVALTVK
                     PESRDAAVAATLEEIRRFLTGGMTPKELADEKSAHAGRFKVDLGSNGGLAHAIDAAVY
                     YGLGIAYLDRFPSLVAAVTKEEADAAFGKRVDPDRFTIVSAGSFENP"
     gene            3692..3967
                     /locus_tag="VF958_10970"
     CDS             3692..3967
                     /locus_tag="VF958_10970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9149671.1"
                     /translation="MPEDRTNDGKTWFRLRLDSEPSPEWMRAYRAGLLGMTRDDREAI
                     LRFEFQGDSVRVAAMEAEVGKLRRVLERRIDAVNAILSGGRGPSPTP"
     gene            complement(3969..5090)
                     /locus_tag="VF958_10975"
     CDS             complement(3969..5090)
                     /locus_tag="VF958_10975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006930593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N(4)-(beta-N-acetylglucosaminyl)-L-asparaginase"
                     /protein_id="HEX9149672.1"
                     /translation="MKGPTRRDLIRSAAIIPVAAQIKSAETRPAPSRPVVIASANEKN
                     PGESGGLAAVKRAYEIIAAGGDPLEAVVSGVNIVENDPDDVTVGYGGIPNEEGVVQLD
                     ASVMDGRTMKAGAVGALERIKNPSRVARLVMEQTNRVFIVGEGALKFARAHGFQEENL
                     LTERSRKIWLYWKENLSGKDDWRPVGNEEEKDPDIRWYIEKYGVADFRPQGTINCDAV
                     TGNGDIVGTTTTCGLFFKIPGRLGDSPVIGAGLYVDNEVGAAGSTGWGEGNLRSCGSH
                     TVVELMRQGKSPEEAALETLRRIVALAKDNRDEGGLPKFQTVFYALNKRGEWGGASVY
                     DGSWGATGRFNRAKYALANASGARLLETAYLYKKEPPKK"
     gene            complement(5106..5516)
                     /locus_tag="VF958_10980"
     CDS             complement(5106..5516)
                     /locus_tag="VF958_10980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9149673.1"
                     /translation="MTNRSIVACVEDLFFRSKIEATARHLNVPVRFAEAKDLAAAAGR
                     DDTAAVLLEVSAEGAPLDVIRRLRQERRTSKLPVIGFLRHTDRELAKQAEGAGFTRVL
                     ARSEFSETLPDLVMDLLAPGTKREIQEEPELPDE"
     gene            complement(5539..6618)
                     /locus_tag="VF958_10985"
     CDS             complement(5539..6618)
                     /locus_tag="VF958_10985"
                     /inference="COORDINATES: protein motif:HMM:NF028240.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative porin"
                     /protein_id="HEX9149674.1"
                     /translation="MRPGILIAALVVCQPVSIGAQEPEYFEQPPAQSRFTLRWEALLR
                     YDFLYQLRVRPDIKRGRFEVRPELAFAPSDRFKVAVRAVGNLGTDDNAQNARNFDNYR
                     SDGAMLDRLYLEARPGAWTVLAGQFGMPLVASEMLWDRDIQTPGAAVSHQIGMGTSTL
                     TFSGAGYYSPQREGDESRIFAGQLVWRGGDIDRLAVEAAAAWWDFDLRNLKSHLVRQN
                     GPETYSGGVRPESGFHLADLLVRVRFPALSLPVTVSVDGVHNFGADGKQKDAFEGALA
                     VGSVGTPGTWRAFYQFQYVERDAVVGAYNTDDWWFHSRYRGHRAGVAVTLAPQIYVQG
                     TFLVQRRLDLRTWLNRLTIDLVKMF"
     gene            complement(6650..>7676)
                     /locus_tag="VF958_10990"
     CDS             complement(6650..>7676)
                     /locus_tag="VF958_10990"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HEX9149675.1"
                     /translation="ELPVESNDEIGILARSFRAMLEELKEKAALEKYVASLSMSLSGE
                     ETLLAPRVTAGGTSGSSLNEPQLGTVFAGRYEIQSVLGRGGMGIVYKAHDRELDDLVA
                     IKTLRGEVLSADPSLLDRFKQEIRLARRITHPNVLRTHDLGEANGLRFLSMEFVKGLT
                     LKHLLESGDILPTPVALRIAKQVCAGLAAAHEVGVIHRDIKPQNIIIEPTGGLKIMDF
                     GIARLTEDRGMTAAGTVIGTPDYMSPEQARGLSLDFRSDIYSTGIVLYEMFTGTLPFE
                     GDSPLAVVLKHVNDPPPLPQLKNPRIDPKIAAIIMKCIEKDPRARFQTVGELYEALAR
                     VTAQHAA"
BASE COUNT         1239 a         2655 c         2508 g         1274 t
ORIGIN      
        1 tttggccggc gatacgcaga cgcggtgggc cctcgttctc ggtgcctcgt ccggcttcgg
       61 cgaggcctgc tcgatcgcgc tcgcgaaggc gggctggaac atcttcggcg tccacctcga
      121 ccgcaaatcc acgcaaccga acgtcgaccg gatcgtcgcg gagatcgccg gcgccggtcg
      181 caaagccgag ttcttcaacg tcaacgcggc cgacgacggg aagcggacgg aggtcgtcgc
      241 cgcgatctcg cgcgcgtgcg ccgacgctcc gccgaaggga cgtgtctcgg tcgtgctgca
      301 ctcgctcgcc ttcgggacgc tcaagccgtt tttcgggcaa ccgcgcgaga acgcgctctc
      361 gaaggcgcag ctcgagatga cgctcgacgt catgggcaac tcgatcgtgt actgggtgca
      421 ggacctcttc ttcgccggcc tgctcgcgcc cggagcgcgc atcttcgcca tgacctccgc
      481 gggggaccac atcgtctggc ccgcgtacgg cgcagtgtcc gccgcgaagg ccgttctcga
      541 atcccacgtc cggcagctgg cggtcgaact tgcgccacac cgcatcgccg tgaacgccct
      601 gcgcgccggc gtcaccgaca ccccggcgct tcgaaagatc ccgggcaatg aacagatgat
      661 cgaacggatc ctggcgatac acccggccgg acgcctgacg acccccgcgg acgtcgcggg
      721 ggcgctcgtc gcgctgtgcg ccgacggggc cgcgtgggtg agcggcaacg tcatcggcgt
      781 cgacggcggc gaggacgtcg ccggggggcg gtagtggagt ccgaagtcca aagtccaaat
      841 tccaaagtcc aggaaacccc ggagatggct ccggttcccg aagcgttcga attcgtccgg
      901 gaaaaagcgg gaatccggga gtttcgtctg cgctcgaacg ggctccgggt gctgctgctc
      961 cgcaacacgg tggccccggt cgcgacgttc gcggtcgtgt accacgtcgg cagccggaac
     1021 gaggcggtcg gccacacggg agcgacgcac ctgctcgagc acctcatgtt caaggggacg
     1081 ccggagttcg accgcgagaa ggccacggcc gtcgccgccg tcctcgaagc gctcggcgcg
     1141 cggttcaacg cgacgacgtg gttcgaccgc acgaactact acgagaccat tccctcggat
     1201 cagatcgaga cggcgatccg gatcgaggcg tcccggatgc gcggcgcata catccgcgac
     1261 tccgaccgcg ctccggagat gacggtcgta cgaaacgagt acgagcgcgg cgagaattcg
     1321 ccgttccagg tcctttacaa ggagacgttc gcaacggcgt tccgcgaaca cccgtaccac
     1381 catccgacga tcggatggcg agcggacatc gaaggcgtca ccaccgagag gttgaaggag
     1441 ttctacgacg tcttctacca cccgaacaac gcgaccgcga tgctgatcgg cgacttcgaa
     1501 gaacccgagg cgctcgccac gctctcccgt cacttcggcc ggatcccgcc gtccgcccgc
     1561 ccgatcccgc cggtctatac gaccgagccc gcgcaggagg gggagcgccg cttcacggtc
     1621 cggcggaccg gacaagtcgc atgggtcgcc ctctcctggc gaaccgtcgc cgcccgccat
     1681 cccgacaccc cggtgctcgc cgcgctcggc aacgtgctcg gcggcggact gacggcccgg
     1741 ctccacctcg cgctcgtaga gacgagcctc gcgctctccg tcacggtcgt cccgtggcag
     1801 ctccgagacc ccgccctgtt ctcggtcttc gctccggtgc gcccgggcgt cgcgccggag
     1861 gacgtcgaaa agaggatccg cgaggagatc acgcgcgtcg cgctgcaagg ggtcaccgaa
     1921 gcggaggcgg agaaggcccg gacgcagatc gaagcggaag tgatcttcga gcgcgactcg
     1981 accgaccagg tcgccgcttc gctttccgag gcaatcgcag tggccgactg ggagtggtac
     2041 gcggattacc cggagatggt gcggcgcgtc accgccgagg acgtccgccg cgtcgccgcg
     2101 acctacttca ccgacgacag cctgacggtc ggcgcctacc ttccgaaaga aaccccggcg
     2161 gcgtgacgat ctcccctgcg gccacggccg ccgtgcgtcg cggcaagatc cccttcgagg
     2221 acgccgtccg gcgccgaacg ctcgcgaatg gagcgcgtct tttcgtgctg gagaaccgct
     2281 tcaacccgac cgtcgccatc tccggatcgt tgaacgccgg acccctcttc gcgccgcccg
     2341 atcgacgtct gctcgcctcc atgaccgccg gtgagctcgc aaaagggacc gagcgccgca
     2401 cgaagctcga gatcgccgaa gagctggaga gccggggcgc gagcttctcg atcgccgcag
     2461 gctcctccga tcccgtgggc gtcgacctcg cggcggcgtc gctctcacgc gacgtggagg
     2521 tgctcctcga cgtgctcgcc gaagtcctcc tttcgcccgt ctttccggcg gaggagctcg
     2581 agaaggagcg gatgcgcctc gtcggcgtgc tgcgggagca gcaggaccag acctcggtgc
     2641 gcgccttcga ggcggcggcg cggcgccttt atccgccggg ccatcctttt caccgccgcc
     2701 gcgccgaaga gcggatcgcg gtcctcgagt ctctcgaacg cgccgaactc cgccgctact
     2761 atgacgagcg ctacggcgcc gccacgttca ttctcgtcgt cgtcggagac gtggattccg
     2821 accgtctcct cgacgggctc gaacgaaggc tgggatcctg gaagccgggt ccggccgcgg
     2881 agatcgagaa gcctccgccg gtcgcgccct cgccgggcgc cgagacggtc gtcatgccgg
     2941 acaaggccag cgccgacgtg gtcctcgcgc agccggcgga cctcgaccgg ctggaccccg
     3001 agtaccttcc gtgcgtgctc gcgaactcgg cgctcgggca gtcgtcgctc acctcgcggc
     3061 tgggcgtccg cgttcgagac accgaaggac tgacctacgg cattcactcg ggcttctccg
     3121 cctcgcacct cgcgggcccc ttcaccgtcg ctctgacggt caagccggag agtcgcgacg
     3181 ccgcggtagc cgcgaccctc gaggaaattc ggcgctttct cacggggggc atgacgccga
     3241 aggagctcgc cgacgagaag agcgcgcacg ccggccgctt caaggtcgac ctgggctcga
     3301 acggcggcct cgcgcacgcc atcgacgcgg cggtctacta cggactcggc atcgcctacc
     3361 tcgatcggtt tccgtcactc gtcgcagcgg tcacgaagga agaggccgac gctgcgttcg
     3421 gcaagcgcgt ggacccggac cgcttcacca tcgtctcggc gggttcgttc gaaaacccct
     3481 gacctgtcat cctgcgcgaa gcgagggatc tcttgcttgc cccggacaga ttcctcgtcg
     3541 cttcgcacct cggaatttca aaagcagggg atagaatccc ctccctgagg aggttcccat
     3601 tcccagacga ccgcaatacc gcaaaccgaa ccgccgccgc cccggggagg cgcctccgac
     3661 ccatcgtgtc tatctctcgc aggagtcggc gatgccggag gaccgaacga acgacggaaa
     3721 gacctggttc cggctccgcc tcgattcgga gccttctccc gagtggatga gggcctatcg
     3781 cgccggactc ctcggcatga cgcgcgacga ccgcgaggcc atcctgcgtt tcgagttcca
     3841 gggcgacagc gtccgggtcg ccgcgatgga agcggaggtt ggcaagctgc gccgcgtcct
     3901 cgagcggcgg atcgacgcgg tcaacgcgat cctgtcgggc gggaggggac cgagcccgac
     3961 cccctgactt acttcttggg cggctccttc ttgtacaggt acgccgtctc gaggagccgc
     4021 gcgcccgacg cgttcgcgag cgcgtacttc gcgcgattga accttcccgt cgccccccac
     4081 gagccgtcgt acacggacgc cccgccccat tccccccttt tgttgagcgc gtaaaagacg
     4141 gtctggaact tcgggaggcc accttcatcg cggttgtcct tcgcgagcgc cacgatccgt
     4201 cgcagcgtct cgagcgccgc ctcctcggga gactttccct ggcgcatgag ctcgacaacc
     4261 gtatgcgagc cgcaggagcg caggttgcct tcgccccagc cggtcgaccc ggcggcgccg
     4321 acctcgttgt ccacgtagag ccccgcgccg atcacgggtg agtcgccgag gcgtcccgga
     4381 atcttgaaga acagtccgca ggtcgtggtc gtaccgacga tgtcgccgtt gcccgtcacg
     4441 gcgtcgcagt tgatcgtccc ctgtggtcga aagtccgcga cgccgtactt ttcgatgtac
     4501 cagcggatgt ccgggtcttt ctcttcctcg tttccgaccg gtcgccagtc gtccttgccg
     4561 gagagattct ccttccagta gagccagatc tttctcgagc gttcggtcag gaggttctcc
     4621 tcctggaatc cgtgcgcccg ggcgaatttg agcgcccctt ccccgacaat gaatacgcgg
     4681 ttcgtctgct ccatgacgag ccgcgcaacc cgggagggat tcttgatccg ctcgagcgcg
     4741 ccgacggcgc ccgctttcat cgtgcgaccg tccatcacgg aagcgtcgag ctgcacgacc
     4801 ccttcttcgt tcgggattcc gccatagccg acggtgacgt cgtccggatc gttctccacg
     4861 atgttcacgc cggagaccac ggcctcgagg ggatcgccgc cggccgcgat gatctcgtaa
     4921 gccctcttca cggcggcgag accgccggac tcgccgggat tcttctcgtt ggcagaggcg
     4981 atgacgaccg gacgcgaggg agcgggacgc gtctccgccg acttgatctg tgcggcgacg
     5041 ggaataatcg ccgcagagcg aatgaggtcg cgccgcgtcg gccccttcat gtccatccgc
     5101 gcgctctact cgtcggggag ttcgggttct tcctggatct cgcgcttcgt gcccggcgcg
     5161 agcaggtcca tcacgagatc gggcagcgtc tcggagaact cgctccgcgc gagcacgcgg
     5221 gtgaagcccg cgccctcggc ttgtttcgcg agctcgcggt ccgtgtggcg gagaaaaccg
     5281 atgaccggca gcttcgacgt ccggcgctcc tgccggagcc gtcggatcac gtcgagcgga
     5341 gcgccttccg ccgagacctc gaggagaacc gcggccgtgt cgtcccggcc ggcggcggcc
     5401 gccaggtcct tcgcctctgc gaagcgcacc ggcacgttca gatgtcgcgc agtcgcctcg
     5461 atcttcgagc ggaagaagag gtcctcgacg caggccacga ttgagcggtt cgtcatggaa
     5521 gtcgggattc tatgcccttc agaacatctt cacgagatcg atcgtcagcc gattgagcca
     5581 cgtcctcaga tcgaggcggc gctggacgag aaacgttccc tggacgtaga tctgaggcgc
     5641 gagcgtcacc gccactccgg cgcggtgccc gcggtagcgc gagtgaaacc accagtcgtc
     5701 ggtgttgtac gcgccgacga cggcgtcgcg ttcgacgtac tggaactggt agaaggccct
     5761 ccaggtcccg ggagtgccga ccgagcccac ggcgagcgca ccctcgaaag cgtccttctg
     5821 cttgccgtcc gccccgaaat tgtggacgcc gtcgaccgac accgttacgg ggagggagag
     5881 cgcgggaaag cgaacgcgca cgaggaggtc cgcgagatgg aagccgctct ccggccgcac
     5941 gcctccactg taggtctccg ggccgttctg acgaacgagg tgtgacttca ggttccgcag
     6001 gtcgaagtcc caccatgcgg ccgctgcctc gaccgccaga cggtcgatgt cgccgccgcg
     6061 ccacacgagc tgtcccgcga agatccgcga ttcgtcgccc tcccgctggg ggctgtagta
     6121 gccggcaccc gagaaagtca gcgtcgacgt gcccatgccg atctgatggg aaaccgcggc
     6181 acccggcgtc tggatgtcgc ggtcccagag catctcgctc gcgacgagcg gcatcccgaa
     6241 ctgcccggcg aggacggtcc aggcgccggg ccgagcctcc aggtagaggc ggtcgagcat
     6301 ggcgccgtcc gagcggtagt tgtcgaagtt gcgggcgttc tgggcgttgt cgtcggttcc
     6361 gaggtttccg accgcgcgga ccgcgacctt gaagcggtcc gacggcgcga atgcgagctc
     6421 cggccgcacc tcgaagcgcc cgcgcttgat gtcgggtcgg acccgcagct gatacaggaa
     6481 gtcgtagcgg aggagcgcct cccaccggag cgtgaatctc gattgcgcag gaggttgttc
     6541 gaagtactcg ggctcctgcg cgccgatcga gacgggctgg cagacgacca gagcggcgat
     6601 caggattccc ggtctcacga cttccgactc ccgattcccg gtttccgact caagccgcgt
     6661 gctgcgccgt cacccgcgcc agcgcctcgt acaactcgcc gaccgtctgg aagcgcgcgc
     6721 gcggatcttt ttcaatgcac ttcatgatga tggcggcgat cttgggatcg atcctcgggt
     6781 ttttcagctg cgggagcggt ggagggtcgt tgacgtgctt cagcacgacg gcaagcgggc
     6841 tgtcgccttc aaaaggcaga gttccggtga acatctcgta gagcacaatc cccgtggagt
     6901 agatgtcgga gcgaaagtcg agcgaaagcc cccgcgcttg ttccggcgac atgtagtcgg
     6961 gggtgccgat gaccgtgccg gcagccgtca tcccgcgatc ctcggtcagg cgggcgatac
     7021 cgaaatccat gatcttcagc ccgccggtcg gctcgatgat gatgttctgc ggcttgatgt
     7081 cgcggtggat gacgcccacc tcgtgcgcgg ccgcgagccc ggcgcagacc tgcttggcga
     7141 tgcgaagcgc caccggcgtc ggcaggatgt cccctgattc gagcaggtgc ttcagggtga
     7201 gccccttcac gaactccatc gagaggaacc gcagtccgtt cgcctctccg agatcatgcg
     7261 tgcgcagcac gttcggatgg gtgatccgcc gggcgagtcg gatctcctgc ttgaagcggt
     7321 cgagaaggga cggatcggcc gacagcacct caccacgcag cgtcttgatg gcgacgagat
     7381 cgtcgagctc gcggtcgtgc gccttgtaca cgatccccat tccccccctg ccgagcaccg
     7441 actggatctc gtatcggccg gcgaagaccg tgccgagctg cggctcgttc aatgagctcc
     7501 cggacgttcc gcctgccgtc acgcggggcg caagaagggt ctcctctccg ctgagcgaca
     7561 tcgacagcga cgcgacgtac ttctccagcg ccgccttttc cttcagctcc tcgagcatcg
     7621 cgcggaagga gcgggcgagg atcccgatct cgtcgttcga ctcgacgggc aactcg
//
LOCUS       DASWAV010000524         1588 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U2665
            k141_668620, whole genome shotgun sequence.
ACCESSION   DASWAV010000524 DASWAV010000000
VERSION     DASWAV010000524.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960316
            Sequence Read Archive: SRR10260007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 1588)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1588)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:47:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,929
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1588
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_668620"
                     /isolate="SMAG_U2665"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.36444 N 132.31889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..233)
                     /locus_tag="VF958_10995"
     CDS             complement(<1..233)
                     /locus_tag="VF958_10995"
                     /inference="COORDINATES: protein motif:HMM:NF014035.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TSUP family transporter"
                     /protein_id="HEX9149676.1"
                     /translation="MPRRSGGSSPPGSRRSRPTIRRQRSRCATERWPPNRKDTPMQML
                     GLVAVGVVAGVAAGMFGIGGGLIIVPALVLLYG"
     gene            complement(127..909)
                     /locus_tag="VF958_11000"
     CDS             complement(127..909)
                     /locus_tag="VF958_11000"
                     /inference="COORDINATES: protein motif:HMM:NF015000.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HEX9149677.1"
                     /translation="MSEPRRTESASEWREPHPVWVVGHRGAPRRARENTIDSFDWAES
                     IGVDAVEFDVRQTRDGEAVVFHDEDVSLGTQRVPVRSFTTREIHRLLLPSDYGDFAIP
                     RLQDVFHRYGRSLAYVVEVKASPGYDRGVMGRRIAGLAAEYGVAGRVLVASFDAEFLK
                     RMREIEPRLATSYIFDHPVALPEPARLTPLFPPVDAIAPAKDLATPPLIAQAAAAGLS
                     VHPWTVNDPEEIRRFLAARVASITTDDPETALALRNGALAAQ"
     gene            complement(906..1583)
                     /gene="pyrF"
                     /locus_tag="VF958_11005"
     CDS             complement(906..1583)
                     /gene="pyrF"
                     /locus_tag="VF958_11005"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002443113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0044205 - 'de novo' UMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HEX9149678.1"
                     /translation="MALDFATREEILAAARRFGAKAGWLKVGLEAFTAEGPGIVSDVA
                     NTGARVFLDLKLHDIPTTVARAVTAAARTGAAMLNVHALGGREMLTAARESAATAGAV
                     KLIAVTLLTSLDPRALADLPIAGHPEGIARRLAVLAKECGLDGVVCAPRDLPAVKGAC
                     GREFLAVVPGIRPAGSEASDQKRIATPAAALALGADLLVVGRPITAAPDPDEALESLL
                     AEIQSRA"
BASE COUNT          237 a          561 c          572 g          218 t
ORIGIN      
        1 atgccataga gcagcacgag cgcggggacg atgatcaggc cgccgccgat cccgaacatt
       61 cccgccgcga cgcccgcgac gactccgacc gccaccaacc cgagcatctg catcggcgtg
      121 tcctttctat tgggcggcca acgctccgtt gcgcagcgcg agcgctgtct ccggatcgtc
      181 ggtcgtgatc gacgcgaccc tggcggcgag gaacctccgg atctcctcgg gatcgttcac
      241 cgtccagggg tgcacggaga gtcccgccgc ggcggcctgc gcgatcaaag ggggcgtcgc
      301 gaggtccttc gcgggcgcga tcgcgtcgac cggcgggaag agcggagtga gacgcgccgg
      361 ctcgggcagc gccacggggt gatcgaagat gtagctggtc gcgagcctgg gctcgatctc
      421 gcgcatgcgt ttcaggaact ccgcgtcgaa ggaggccacg aggacgcgcc cggcgacgcc
      481 gtactccgct gccaggccgg cgattcggcg gcccatcacc ccgcggtcgt aaccgggtga
      541 ggccttcacc tcgacgacgt aggcgagcga gcgcccatat cggtgaaaga cgtcctggag
      601 ccgcgggatc gcgaagtccc cgtagtcgga aggcaggagg aggcggtgga tctcccgggt
      661 cgtgaagctc cggaccggaa cccgctgcgt accgagcgac acgtcctcgt cgtggaagac
      721 gacggcctct ccgtcacggg tctgccggac gtcgaactcg acggcgtcga cgccgatgct
      781 ctcggcccag tcgaagctgt cgatcgtatt ctcgcgcgcg cgacgcggcg ctccgcggtg
      841 gccgacgacc cagaccgggt ggggctcccg ccactcgctc gcagattccg tccgccgcgg
      901 ctcgctcacg ctctcgattg tatttccgcg agcagtgact ccagggcctc gtcgggatcg
      961 ggcgcggccg tgatcggtcg gccgacgacg agcaggtccg ctcccagggc gagcgcggcg
     1021 gcgggcgtcg cgatgcgctt ctgatcggag gcctcgctcc ctgcggggcg gatgcccgga
     1081 acgacggcga ggaactcgcg accgcacgcg cccttgaccg cgggaaggtc gcgcggggcg
     1141 caaacgaccc cgtcgagacc gcactccttc gcgagcacag ccagccgccg ggcgatgccc
     1201 tccgggtgcc ccgcgatcgg aaggtccgcg agcgcgcgcg gatcgagcga cgtgaggagc
     1261 gtgacggcaa tcagcttgac cgcgcccgcg gtcgccgccg actcccgggc ggcggtcagc
     1321 atctcgcgtc cgccgagcgc gtggacgttc aacatcgccg cgccggtccg cgcggccgcg
     1381 gtgaccgcgc gcgcgaccgt cgtcggaatg tcgtggagct tgagatccag gaacacgcgc
     1441 gcgccggtgt tcgcgacgtc ggagacgatg ccggggccct cggccgtgaa cgcctcgagt
     1501 ccgaccttca gccacccggc tttggcgccg aagcggcgcg ccgccgcgag gatctcctcg
     1561 cgcgtcgcga aatcgagcgc gatgcaga
//
LOCUS       DASWAV010000525         3654 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U2665
            k141_270238, whole genome shotgun sequence.
ACCESSION   DASWAV010000525 DASWAV010000000
VERSION     DASWAV010000525.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960316
            Sequence Read Archive: SRR10260007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 3654)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3654)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:47:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,929
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3654
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_270238"
                     /isolate="SMAG_U2665"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.36444 N 132.31889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..737
                     /locus_tag="VF958_11010"
     CDS             <1..737
                     /locus_tag="VF958_11010"
                     /inference="COORDINATES: protein motif:HMM:NF013595.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="NHL repeat-containing protein"
                     /protein_id="HEX9149679.1"
                     /translation="VADTKNNRIQVFDTNRQFVRSLGTAGSADGQLKEPCGVTVDSDG
                     SVIVADTWNHRIARFGADGAWLGAFTDAERGLFGPRAVLMSRDSLYVADTGNKRIVRF
                     DRKWNRVGEWGTSGSGPGQFVEPVGLAADAAGNVYVADTGNHRIQVFDGEGHFLREFA
                     VFGWKDFYTEPYIAIGPADSVLATDSTEGRVNEYDSSGNLHRSWRSDGEFKKPTGLAV
                     DSFGRVSVSDRETHRVVTWNLGDVLR"
     gene            complement(801..2156)
                     /locus_tag="VF958_11015"
     CDS             complement(801..2156)
                     /locus_tag="VF958_11015"
                     /inference="COORDINATES: protein motif:HMM:NF013739.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding protein"
                     /protein_id="HEX9149680.1"
                     /translation="MASIAILGAGPTGLGAAYRLREHGVDDFEIFERAGQIGGLATSF
                     EDPKGYTWDVSGHIIFSGYPYFNEFLEKMLGKEGVRRIDRESWIKFEDRYVRYPFQNH
                     LSSLPEEAMIECLIGLIESQTIDRDRAFRNFEEWVYAKFGAGVAKHFMVPYNLKVWAT
                     PLSRMGFYWIAERVSVVDWKRALETTLAPQNVDWGPNATFGYPATKGTLGLWLAVLPH
                     IGDRVRYRKRAVCVDEDKREIRFSDGSSARYEKLLTTLPLEAFVARLAHAPDEVRAAT
                     KKLLFNRLFSVGVGLKRPSPSTKNWIYFPNPKTPFYRMTYLSNYSPDIVPGGDTSRYC
                     SLLMETTYSRFRPLPQGDFAKSVIEGLVAEGILEPGDLPFIESVYLIHAGHAYPIPSV
                     ERDPALATIQSYLESKEIYSRGRFGSWKYEIGNQDHSLMQGKEIVDRWLDGSEERVFR
                     T"
     gene            complement(2173..2925)
                     /locus_tag="VF958_11020"
     CDS             complement(2173..2925)
                     /locus_tag="VF958_11020"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEX9149681.1"
                     /translation="MSAEKEPQLGVGFLAYNEEGSIEKTVAEAVASLSAIDGLDWRVL
                     VVNDGSRDRTGEIADSLAARDPRVSVVHHDGNKGYAVATETALRNTPGEVVMVVDGDG
                     QHTMGDAPRFLEAISQGADVVFGWKKKRYDPWPRLILSKGLNTLSRVLLGSPLHDING
                     GCRAFRKDVARRIEIRHRINFVGDEIFARCRIAGWKVAEVVVRHFPREAGQSIHRPWK
                     MLGTITLVIRYLFELRGEIHRADAMKRLVRTE"
     gene            complement(2915..>3654)
                     /locus_tag="VF958_11025"
     CDS             complement(2915..>3654)
                     /locus_tag="VF958_11025"
                     /inference="COORDINATES: protein motif:HMM:NF013226.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="UbiA family prenyltransferase"
                     /protein_id="HEX9149682.1"
                     /translation="CTVSSAVYLINDVMDRERDRAHPVKKLRPLASGRLRVSTAVSAS
                     IVLVTVGMLGAFLIQPRFGLWSLFYLVLMIAYSLWLKSIPFLEALIVASGMPLRALAG
                     AALAGVPPSTFLMVCAYLLALFLVVGKRQWELQRAGHRTASEHRAVLAKYRSEGLDFL
                     FIVTALTTVASYGAYSVAPHTLQFHGHSFGATVPFVVLGVARYVRLVYRRGGGGNPTQ
                     ALLIDDPWLLLIVLGWVAVAGWIIYVR"
BASE COUNT          695 a         1172 c         1158 g          629 t
ORIGIN      
        1 acgtcgcgga tacgaagaac aaccggatcc aggtgttcga taccaaccga cagttcgtgc
       61 ggagcctcgg caccgccggc tctgccgacg ggcagctgaa ggagccgtgc ggcgtcactg
      121 tggattcgga cggaagcgtg atcgtggccg atacgtggaa tcaccgcatc gcccgattcg
      181 gcgccgatgg tgcctggctc ggcgccttca ccgacgcgga gcgcggcctc ttcggcccgc
      241 gcgccgtgct catgtcgcgc gactctctct atgtcgcgga taccgggaac aagcggatcg
      301 tccggttcga taggaaatgg aaccgagtcg gcgagtgggg aacctcgggc agcggtcccg
      361 gccagttcgt ggagccggtc ggtctcgcgg ccgacgccgc cggcaacgtt tacgtagcgg
      421 acaccggcaa ccatcgcatc caggtcttcg acggggaggg acacttcctc cgcgaattcg
      481 ccgtcttcgg ctggaaggac ttctacaccg agccttacat cgcgatcggt ccggccgact
      541 ccgttctcgc aaccgactcg accgaaggtc gcgtcaacga gtacgactcc tcgggcaacc
      601 tgcaccgctc ctggcgctcc gacggcgaat tcaagaaacc taccggtctg gcggtcgact
      661 cgttcggtag agtgagcgtg tccgaccgcg aaacccatcg cgtcgtcacg tggaacctgg
      721 gcgacgttct ccgttgatca ggaggacgag agatcgctgc gagtgcgttc tctccgatgc
      781 tccgtcgttc ggatgatcga ctaggtacga aagacccgtt cctccgagcc gtcgagccag
      841 cgatctacaa tttctttgcc ctgcatcaaa gagtgatcct gattgccgat ctcgtacttc
      901 caggacccga accgcccgcg cgaatagatc tctttcgact cgagatacga ctggatcgtc
      961 gcgagtgcgg ggtcgcgttc gacgctcggg atcgggtaag cgtgaccggc gtggatcagg
     1021 tataccgact cgatgaaagg caggtcgccg ggctccagaa tcccctcggc gacgagacct
     1081 tcgatcaccg acttcgcgaa gtccccttgg ggcagcgggc ggaagcgcga gtacgtcgtc
     1141 tccatgagga gcgagcagta gcgcgaagta tcgccgcccg gcacgatgtc gggcgagtaa
     1201 ttcgagagat aggtcatccg gtagaacggc gtcttgggat tcggaaagta gatccagttc
     1261 ttcgtcgacg gcgacgggcg cttgagtccc acgccgacag aaaagaggcg gttgaagagt
     1321 agcttcttcg tagccgcgcg gacctcgtcc ggcgcgtggg ccagtcgggc gacgaacgcc
     1381 tccagcggaa gcgtcgtcag gagcttctcg taacgcgccg aagagccgtc cgagaagcgg
     1441 atctcccgct tatcctcgtc gacacagacc gcgcgcttgc ggtaccgcac gcggtccccg
     1501 atgtgcggca ggacagcgag ccagagtccg agcgtaccct tcgtcgccgg gtagccgaac
     1561 gtcgcgttcg gtccccagtc gacgttctgc ggagcgagcg tcgtctcgag cgccctcttc
     1621 cagtccacga cgctcacccg ctcggcgatc cagtaaaagc ccattctcga caacggtgtc
     1681 gcccagacct tcaggttgta cgggaccatg aagtgcttcg cgacgccggc gccgaacttc
     1741 gcgtacaccc actcctcgaa gttgcggaac gctcggtccc gatcgatcgt ctgcgattcg
     1801 atgagaccga tcaggcactc gatcattgcc tcttcgggga gcgacgagag gtggttctgg
     1861 aagggatagc ggacgtagcg gtcttcgaac ttgatccagc tctcgcgatc gatgcgccgc
     1921 acgccctctt tccccagcat cttctcgagg aactcgttga agtaggggta gcccgagaag
     1981 atgatgtggc ccgacacgtc ccacgtgtat cccttggggt cttcgaagct cgtggcgagc
     2041 ccgccgatct gcccggctcg ctcgaagatc tcgaagtcgt cgacgccgtg ctcgcgcagc
     2101 cggtaggccg cgccgaggcc ggtcggtccc gctcccagga tcgcgataga cgccatcaga
     2161 cgatcgagtg ctctactcgg tccggaccag acgtttcatc gcgtcggcgc gatggatttc
     2221 cccgcgcagc tcgaagagat aacggatgac gagagtgatc gtgccgagca ttttccatgg
     2281 ccggtgaatg ctctgccccg cttcacgggg gaagtggcgg acaaccacct ccgcgacctt
     2341 ccagccggcg atccgacaac gcgcgaagat ctcgtctccc acaaaattga tccgatgccg
     2401 gatctcgatg cgccgggcga cgtccttgcg gaacgcgcga cagccgccgt tgatgtcgtg
     2461 gagcggcgac ccaaggagga cgcgcgagag cgtgttcaag cccttcgaga ggatgagacg
     2521 gggccacggg tcgtatcgtt tcttcttcca accgaagacg acgtccgcgc cctgtgaaat
     2581 cgcctcgagg aatcgaggcg cgtcgcccat cgtgtgctgt ccgtcgccgt ccaccaccat
     2641 cacgacctcg cccggggtgt tgcgcaacgc ggtctccgtc gcgacggcgt accccttgtt
     2701 cccatcgtga tgcacgacgg agactctcgg gtcccgggca gccagtgagt cggcgatctc
     2761 gcccgtccga tcgcgacttc cgtcgttcac gaccagtacg cgccagtcga ggccgtcgat
     2821 cgccgaaagt gaagccaccg cttccgcgac cgtcttctcg atcgatccct cctcgttgta
     2881 ggcgaggaag ccgacgccga gttgcggctc cttctcagcg gacatagatg atccagcctg
     2941 caaccgcgac ccagccgagc acgataagca gaagccaggg atcgtcgatc aacagcgcct
     3001 gcgtggggtt gcctccaccc ccccgccggt agacgagtcg aacgtagcgc gcgaccccca
     3061 ggacgacgaa cggaacggtt gcaccaaagg agtgcccgtg gaactggagc gtgtgcgggg
     3121 ccacgctgta ggcgccgtag gacgcaacag tagtgagagc ggtgacgatg aagaggaaat
     3181 caaggccttc cgacctgtat ttcgcaagga cggcccgatg ctcggacgcc gtcctgtgtc
     3241 cggcccgctg gagctcccac tgtctcttgc cgacgacgag aaagagagcg agcaggtagg
     3301 cgcagaccat gaggaaggtc gacgggggta cgcccgcgag cgccgccccg gcgagcgccc
     3361 ggagcggcat tcccgacgct acgatgagtg cctcgaggaa ggggatgctc tttagccaca
     3421 gcgaataggc gatcatcagc accagataaa agagcgacca gaggccaaaa cgcggctgga
     3481 tcaggaacgc cccgagcatt cccaccgtca cgagtacgat cgaagccgag accgcggtcg
     3541 agacccggag ccgtcccgaa gcgagcggcc gcagcttctt gacgggatgg gcgcggtccc
     3601 gctcccgatc catgacgtcg ttgatgagat acacggcgct cgacacggtg caga
//
LOCUS       DASWAV010000526         2867 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U2665
            k141_348703, whole genome shotgun sequence.
ACCESSION   DASWAV010000526 DASWAV010000000
VERSION     DASWAV010000526.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960316
            Sequence Read Archive: SRR10260007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2867)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2867)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:47:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,929
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2867
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_348703"
                     /isolate="SMAG_U2665"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.36444 N 132.31889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..666)
                     /locus_tag="VF958_11030"
     CDS             complement(<1..666)
                     /locus_tag="VF958_11030"
                     /inference="COORDINATES: protein motif:HMM:NF013134.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD binding domain-containing protein"
                     /protein_id="HEX9149683.1"
                     /translation="MRTAASSLELLQPRTLRDALRMMRDEQPLTPLAGCTDVYVGLNF
                     GTLPVKRFIDLSPLDELREIRVADGALSIGALATYTRIIRSALVRRRVSILAAAAREI
                     GGVQIQNRGTLGGNVANGSPAGDTLPVLAVSDATLVLGSARGERRVPFNSFYTGYRKS
                     VLRADELILAIEIPRLAGRPWFRKVGTRAAQAISKVVMAAVRSDRPRVALGSVAPTVV
                     RLPE"
     gene            complement(663..1154)
                     /locus_tag="VF958_11035"
     CDS             complement(663..1154)
                     /locus_tag="VF958_11035"
                     /EC_number="2.1.1.100"
                     /EC_number="2.1.1.334"
                     /inference="COORDINATES: protein motif:HMM:NF016107.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenylcysteine carboxylmethyltransferase
                     family protein"
                     /protein_id="HEX9149684.1"
                     /translation="MLVLRALFFALLMPGAVVIGIPWLLLRGQPSRDVRLDFPAVIGS
                     ALVIAGAATLFVCIRDFAVSGRGTLAPVDPPRKLVRVGLYRHVRNPMYLGVLLVLVGE
                     SLFFESPVLGFYAAVIALSFHLFVVLFEEPSLRGLFGPDYERYRASVPRWVPRLRPWE
                     GRP"
     gene            complement(1155..1610)
                     /locus_tag="VF958_11040"
     CDS             complement(1155..1610)
                     /locus_tag="VF958_11040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020716417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HEX9149685.1"
                     /translation="MRFTLNGKTRDAKAPPMKRLLDVLREDFGLTGTKEGCGEGECGA
                     CTVLVDGLPVNSCLVPFAQVRGSAVTTIEGLRGRHPLQAAFVGEGGAQCGICTPGMIL
                     AGAALPRGASKEEVRAGLAGNICRCTGYTAIYRAVQKVAKRRAPRGRRS"
     gene            complement(1607..>2867)
                     /locus_tag="VF958_11045"
     CDS             complement(1607..>2867)
                     /locus_tag="VF958_11045"
                     /inference="COORDINATES: protein motif:HMM:NF042649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="molybdopterin cofactor-binding domain-containing
                     protein"
                     /protein_id="HEX9149686.1"
                     /translation="DGGAYMTLSPVVLSRGVIHATGPYRCDNVRVNGRVVMTNTPPNG
                     AFRGFGAPQTQFAAEVHMERIAEALGMDPVRLREVNAFRPGDTTATGQKLGSDCSAVK
                     VLREAVRKSGFREKRRAWRGTDRGIGLSLFFHGSGFTGGGEVKLASKASLELTATGAR
                     ILVASTEIGQGTRTMHAQIVADALGIPYDRVEVEAADTARVPDSGPTVASRTCMVVGK
                     ILERCAIEIRQKLGKMSAREYLSRYGSLVVTKEYAGPDGLYWDDETYRGDAYGAFGWG
                     CDVVEVERDPVTFEIRPMRVTTVQDIGKAIHPILAGGQIEGGTAQGLGYALLEEVAMR
                     DGRMANAQLTNYIVPTTLDTPDIDATILENPYEHGPYGAKGVGEMPIDGPAPAVVNAL
                     RSLGLDVREIPATPERVMAASSPSTAA"
BASE COUNT          468 a         1003 c          984 g          412 t
ORIGIN      
        1 ctcgggcagc cgcacgacgg tcggcgccac gctgccgagc gccactcgcg gacgatccga
       61 gcgcaccgcc gccatgacga ccttggaaat cgcctgcgcg gcgcgggtcc cgaccttccg
      121 gaaccacgga cgccccgcga ggcgcgggat ctcgatcgcg agaatgagct cgtcggcgcg
      181 gagcacgctc ttgcggtacc ccgtgtagaa ggaattgaac gggacccgcc gctcgccgcg
      241 cgcgctgccg agaacgagag ttgcgtcgga cacggcgagg accggcagcg tgtcgccggc
      301 cggagacccg ttcgcgacgt tgccgccgag agtgccgcgg ttctggatct gcacgcctcc
      361 gatctcgcgg gcggcggccg cgagaatcga aacgcggcgc cgcacgagcg ccgagcggat
      421 gatccgggta taggtggcga gcgcgccgat cgagagggcg ccgtcggcca cgcggatctc
      481 gcgaagctcg tcgagtggcg acaggtcgat gaagcgcttc accggcagag tcccgaagtt
      541 cagcccgacg taaacgtccg tgcaacccgc gagcggcgtg agcggctgtt cgtcgcgcat
      601 catgcggagc gcgtcgcgca aggtgcgggg ctggaggagt tcgagagaag acgcggccgt
      661 tctcaaggcc ttccctccca tgggcggagg cgcggaaccc agcgtggaac ggacgcgcga
      721 tatcgctcgt agtccgggcc gaacaaaccg cgcaggctcg gctcctcgaa cagcacgacg
      781 aacaggtgaa acgagagagc gatgaccgcg gcgtagaagc cgaggaccgg agactcgaag
      841 aagagcgact ccccgacgag cacgagcagc acgccgagat acatcgggtt gcgcacgtga
      901 cggtagaggc cgacccgcac cagcttccgg ggaggatcca cgggcgcgag cgtgccccgc
      961 cccgaaaccg cgaagtcgcg gatgcagacg aagagcgtcg cggcgcccgc gatcacgagc
     1021 gcggaaccga tgaccgccgg aaagtcgagc cggacgtccc gcgagggctg gccgcgcagg
     1081 aggagccacg ggatcccgat cacgaccgcg cccggcatga gaagcgcgaa gaagagggcg
     1141 cgcaggacga gcattcagct ccgccggccc cggggagccc gtctcttcgc gaccttctgg
     1201 acggcgcggt agatggccgt atagccggtg catcggcaga tgttgccggc gaggccggcc
     1261 cggacttctt ccttgctcgc cccgcggggg agcgccgccc cggcgaggat catccccggc
     1321 gtgcagatcc cgcactgcgc gccaccctcg ccgacgaaag cggcctggag cgggtgacgg
     1381 ccgcgcaggc cttcgatcgt cgtcacggcg gaaccgcgaa cctgcgcgaa cgggacgaga
     1441 caggaattca cgggaaggcc gtccaccagc accgtgcacg ctccacactc cccttcgcca
     1501 catccctcct tcgtgccggt caggccgaag tcttcgcgca ggacgtcgag caaccgcttc
     1561 atcggcggcg cttttgcgtc gcgggtcttt ccgttgagcg tgaacctcac gcggccgtcg
     1621 agggcgaaga cgccgccatg acccgctcgg gcgtcgccgg gatctcgcgc acgtcgagcc
     1681 cgaggctgcg aagggcgttg acgacggcag gcgcgggtcc gtcgatgggc atctctccga
     1741 cgcccttcgc gccgtatgga ccgtgctcgt acgggttttc gagaatcgtc gcgtcgatgt
     1801 ccggagtgtc gagcgtcgtg gggacgatgt agttcgtgag ctgcgcattt gccatacgtc
     1861 catcgcgcat cgcgacctct tccagcaagg catagccgag cccctgcgcg gtgcccccct
     1921 cgatctggcc gcccgcgaga atcggatgaa tcgcttttcc gatgtcctgg accgtcgtca
     1981 cgcgcatcgg gcggatctcg aacgtgaccg gatctctctc gacctcgacg acgtcgcatc
     2041 cccaaccgaa ggcgccgtac gcgtcgcccc ggtacgtctc gtcgtcccag tagagcccgt
     2101 ccgggcccgc gtactccttc gtcacgacga gcgacccgta cctcgacagg tactcgcggg
     2161 ccgacatctt cccgagcttc tgccggatct cgatggcaca gcgctcgaga atttttccga
     2221 cgaccatgca ggtccgcgag gcgaccgtcg ggccgctgtc ggggacgcgc gcggtgtccg
     2281 ccgcctcgac ctcgaccctg tcgtagggaa tccccagcgc gtccgcgacg atctgcgcgt
     2341 gcatcgtgcg ggtcccctgg ccgatctccg tggacgcgac gaggatccgc gccccggtcg
     2401 ccgtcagctc gagcgaggcc tttgacgcga gcttgacctc gccaccaccg gtgaaaccgg
     2461 agccgtggaa gaagagcgac aagccgatcc cgcggtcggt gcctcgccac gcgcggcgct
     2521 tctcccggaa cccgctcttc cggactgctt cacggagcac cttcaccgcg ctgcagtcgc
     2581 tcccgagctt ctgcccggtc gcggtcgtgt ctcccggccg aaacgcgttg acttccctca
     2641 gccggaccgg atccatgccg agcgcttcgg cgatccgctc catgtggact tcggcggcga
     2701 actgcgtctg cggcgcgccg aagccgcgga acgcgccgtt cggcggcgtg ttggtcatca
     2761 cgacccggcc gttgacgcgg acgttgtcgc accggtacgg cccggtcgcg tggatgaccc
     2821 cgcgggagag gacgacgggg gagagcgtca tgtacgcgcc gccgtcc
//
LOCUS       DASWAV010000527         3922 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U2665
            k141_586634, whole genome shotgun sequence.
ACCESSION   DASWAV010000527 DASWAV010000000
VERSION     DASWAV010000527.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960316
            Sequence Read Archive: SRR10260007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 3922)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3922)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:47:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,929
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3922
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_586634"
                     /isolate="SMAG_U2665"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.36444 N 132.31889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..164)
                     /locus_tag="VF958_11050"
     CDS             complement(<1..164)
                     /locus_tag="VF958_11050"
                     /inference="COORDINATES: protein motif:HMM:NF037651.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyl transferase
                     subunit beta"
                     /protein_id="HEX9149687.1"
                     /translation="MWFRKDKAPKEPREKRSKVPEGLWVKCENCREILYNKELARNFK
                     ICTKCGYHFRL"
     gene            complement(255..1040)
                     /locus_tag="VF958_11055"
     CDS             complement(255..1040)
                     /locus_tag="VF958_11055"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX9149688.1"
                     /translation="MSAVLEARGLCASYGATRALDGLDAELLSGELVALLGENGSGKS
                     TFLKVVARIVPPEGGELRLDGRPLESVGRRESARRIAYLPQSPDLVFPIRCLDLVLQG
                     RAPHSRTFSADSAEDHDRALAAMRTCDVEHLAGRDAAEISGGERRRVFLARAVAQAAG
                     VWLLDEPTSGLDPRHRLEFLELLARTHAERATTVVLVTHEIDLAGEVADRVILLRRGR
                     ALASGPPAETLTRERLRETFDVESRIETDAAGRRRIVPYAPSR"
     gene            complement(1045..2022)
                     /locus_tag="VF958_11060"
     CDS             complement(1045..2022)
                     /locus_tag="VF958_11060"
                     /inference="COORDINATES: protein motif:HMM:NF013219.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HEX9149689.1"
                     /translation="MKPVRRRAAAVGGALLLLATTALLGLVVGGVRLSLSDLVAGEGP
                     AGRAARLVASLRAPRVALSALVGACLALAGAALQALLKNPLADPFLLGTSGGAAVGAA
                     LATVAGLSPFVSPAAAFAGAIGSSVAVAALARRGGRLDLQRLLLAGLIANAFFSAVLL
                     AVFSVASGQTARTMLFWMMGSLADATPGRVIALAPYAAAALAVLLAFGSRLNLFAVGE
                     ENAAALGVDVEKAKRVVFLASSLATGAAVAFAGIIGFVGLLVPHAARSVVGNDQRTLL
                     PVSAIAGAALLVAADALSRSAFAPAELPVGAVTAAIGAPVFAWLLFRSA"
     gene            complement(2019..3005)
                     /locus_tag="VF958_11065"
     CDS             complement(2019..3005)
                     /locus_tag="VF958_11065"
                     /inference="COORDINATES: protein motif:HMM:NF013648.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helical backbone metal receptor"
                     /protein_id="HEX9149690.1"
                     /translation="MHAPWHPQRPRRGRVARLTGRGYTPPAGDPHSSARRFVLVIAAI
                     AALLACRRASPPRSVAPIEPRRVVALAPNLTEIVFALGAGGSLVGVSEYSDFPAAARA
                     IPRVGGLEVSAEKVASLTPDLVLATVEGNARGPVSALLAAGLRVVVVPTGSLDAVLET
                     IRRTGEALGRSARAERLVAELVRRRAAVRARVAGRTRPRAILLVWPDPPQAAGGGTFL
                     DDVLQEAGAENLVGNRPGWPVLSAEYLTTAPVDALVLPMSAANLPTFERAFASGALSR
                     GEARRARVIRLDEAALTRPGPRVFDALEELAAQLSTAESTPSPPPAPGGRGR"
     gene            complement(2951..3175)
                     /locus_tag="VF958_11070"
     CDS             complement(2951..3175)
                     /locus_tag="VF958_11070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9149691.1"
                     /translation="MSLSPDQRKFYEHTLEMTRREISEVEGQIQEELAKVKDRLAELQ
                     TAQKAARQMYDAACMRLGIPNDLEEAESQG"
     gene            complement(3261..>3922)
                     /locus_tag="VF958_11075"
     CDS             complement(3261..>3922)
                     /locus_tag="VF958_11075"
                     /inference="COORDINATES: protein motif:HMM:TIGR01352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0031992 - energy transducer activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HEX9149692.1"
                     /translation="LEVSPPPVPVGTPAVIPAAALDSRAIQQEVQRQLAQKRQELQRQ
                     PAPVARPIAASARPSPVPAVAPPTEVPAVRAEPTSPPPPPTVPPPEPTEVVAAAPPPE
                     AVPAPPPRQAAPAEAEVSRGDLVGPGPGVVEPALLAPPHVNYSPLARQQRVSGRVVIL
                     VLVTEDGRAADARVQKGIGGRSGIDGAVLDAVRTSRFRAATKNGVPVKMWRTVVVDVK
                     P"
BASE COUNT          614 a         1375 c         1410 g          523 t
ORIGIN      
        1 agccggaagt ggtagccgca cttcgtacag atcttgaagt tccgtgcgag ctccttgttg
       61 tagaggatct cgcggcagtt ctcgcacttg acccagagcc cctcggggac cttgctgcgc
      121 ttctcacggg gctccttggg agccttgtct tttcggaacc acatggcgga accgggaatg
      181 gtagtccaaa gtcgggaacc ctgctctttc gcccggctgc gggtgtcgag agaggttggt
      241 cggcgccagc cggttcagcg ggacggcgcg tacgggacga tcctccttct cccggcggcg
      301 tcggtctcga ttcggctctc gacgtcgaag gtctcgcgaa gccgctccct cgtgagcgtc
      361 tcggccggcg ggccggacgc gagcgccctc ccccgccgca ggagaatgac ccgatccgcc
      421 acctcccccg ccaggtcgat ctcgtgcgtc acgagcacca cggttgttgc gcgctcggcg
      481 tgggtgcgcg cgagcagctc gagaaactcg agccgatgcc gggggtcgag cccggacgtc
      541 ggttcgtcga gcagccagac gccggccgcc tgcgcgaccg cgcgcgcgag aaacacgcgg
      601 cgcctctcgc cgccggagat ctccgcggcg tcgcgcccgg cgaggtgctc gacgtcgcac
      661 gtccgcatcg ccgcgagcgc ccggtcgtgg tcctccgcgg agtcggcgga aaaggtccgg
      721 gagtgtggcg cgcgcccctg gaggacgagg tcgaggcagc ggatggggaa cacgagatcg
      781 gggctctgcg ggagatacgc gatccgccgc gcgctctccc gccgtccgac ggactcgagc
      841 ggtcttccgt cgagccggag ctcacccccc tccggaggaa cgatccgtgc gacgaccttc
      901 aggaacgtgg actttcccga gccgttctct ccgaggaggg cgacgagctc gccggaaagg
      961 agttcggcgt ccagtccgtc gagcgcgcgg gtcgcgccgt acgaagcgca gaggcctctc
     1021 gcctcgagaa cggcgctcac ggtttcacgc gctccggaag agaagccacg cgaagaccgg
     1081 tgcgccgatc gcggcggtca ccgcgcccac gggtagttcc gcaggggcga acgccgagcg
     1141 cgacaaggcg tccgccgcaa cgagaagagc cgcgccggcg atcgccgaga ccgggaggag
     1201 cgttcgctgg tcgttaccga cgaccgaccg cgcggcgtgc ggcacgagaa gcccgacgaa
     1261 accgatgatg ccggcgaacg cgactgcggc gcctgtcgcg agcgacgacg cgaggaagac
     1321 gactcgcttc gccttttcca cgtcgacgcc gagcgcggcc gcgttctctt cgccgacggc
     1381 gaaaaggttc agccgcgaac cgaaggcgag cagcacggcg agcgcggctg ccgcatacgg
     1441 ggcgagcgcg atcacgcggc ccggcgtggc gtccgccagg cttcccatca tccagaagag
     1501 catcgtgcga gccgtctgac cggaggccac cgagaacacc gcgaggagga ccgcggagaa
     1561 gaaagcgttc gcgatcagtc cggcgaggag cagacgttgc aggtcgagac ggccgccgcg
     1621 gcgagcgagc gccgccacag cgacggaaga cccgatcgcg cccgcgaatg ccgcagcggg
     1681 cgagacgaaa ggcgaaagac ccgcgaccgt ggcgagcgcg gcgccgacgg ccgcaccgcc
     1741 ggaggtgccg aggagaaacg gatcggcgag cggattcttc aagagcgcct ggagcgccgc
     1801 ccccgcgaga gcgaggcacg cgcccacgag ggcggagagc gccacgcgcg gcgcgcgcag
     1861 cgatgcgacg agccgcgccg ctcgaccggc cggaccctcg cccgcgacga ggtcggaaag
     1921 ggacaggcgc accccgccga ccacgagccc gaggagcgcc gtcgtcgcga gcagcaggag
     1981 agcacccccg acggctgccg cgcgccgacg aacgggcttc atcgccctct ccctccagga
     2041 gcgggtgggg gtgagggggt tgattcggcc gttgacagct gcgccgccag ctcctcgagc
     2101 gcgtcgaaca cgcgcggccc cggccgcgtg agtgccgctt cgtccaaccg gatcactcgg
     2161 gcccgacgcg cctcgccccg cgaaagcgcg ccggaggcga acgcccgctc aaacgtcgga
     2221 aggttcgcag cggacatggg gagaacaaga gcgtctacgg gcgccgtagt caagtactcc
     2281 gcggacagga ccggccaccc gggccggttc ccgacgaggt tctccgctcc cgcttcctgg
     2341 aggacgtcgt cgagaaacgt tcctcctccc gcggcctgcg gggggtccgg ccacacgagg
     2401 agaatcgcgc gggggcgcgt ccgtcccgca actcgcgccc gcacggctgc ccgcctccgc
     2461 acgagttctg ccacgagtcg ctcggctctc gcggaacggc cgagggcctc gcccgtccgg
     2521 cggatcgtct cgagcaccgc gtcgagcgag cccgtcggaa cgaccaccac gcgcagcccc
     2581 gctgcgagaa gcgccgaaac ggggccgcgg gcatttccct cgacggtcgc gaggacgagg
     2641 tcgggagtga gcgaagcgac cttttccgcg ctgacctcga gacctccgac gcgcggaatc
     2701 gcccgtgccg ctgccggaaa gtccgagtac tccgacacac ccacgaggga gcctcccgcg
     2761 ccgagcgcga acacgatttc cgtcaggttc ggagcgaggg cgacgacgcg gcgaggttcg
     2821 atcggcgcta cggatcgcgg cggggaggct cggcgacagg cgagaagcgc ggcgatcgcc
     2881 gcgatgacca gaacgaaccg gcgcgcggag gagtgcggat caccggccgg cggggtgtat
     2941 ccccgaccgg tcagccttgc gactcggcct cttcgaggtc gttggggatg ccaaggcgca
     3001 tgcacgcggc gtcgtacatc tgcctcgccg ctttctgcgc ggtctgcagc tccgcgaggc
     3061 ggtccttgac cttggccagc tcctcctgga tctggccctc gacttcgctg atttccctcc
     3121 gggtcatctc gagcgtgtgc tcgtagaact tccgctgatc gggcgaaagg ctcattcgct
     3181 tcttcctcct gctcgcgcgt gccggtcgat actagaacaa agtcccggct tctggttttc
     3241 gtcgctggac gtcggaccgg tcatggcttc acgtccacca ctacggtacg ccacattttg
     3301 accggcacgc cgttcttcgt cgccgcacgg aaccgcgaag tccggaccgc gtccaggact
     3361 gccccatcga ttccgcttcg gccgccgatg cctttctgga ctcgtgcgtc cgcggccctt
     3421 ccgtcttccg tcacgagcac gagaatgacc acccggccgg aaacccgctg ttgccgcgcc
     3481 aggggcgagt agttgacgtg gggcggagcg aggagcgccg gctcgacgac gcccggaccc
     3541 gggccgacga ggtctccccg cgagacctcc gcttcggctg gcgcggcctg tcggggcgga
     3601 ggggccggca cggcctcggg cggaggcgct gcggcgacca cctcggtggg ctcgggagga
     3661 ggcaccgtcg gaggaggcgg cggagaggtc ggctcggccc ggacggccgg cacctccgtc
     3721 ggcggagcga ccgcggggac cggagagggc ctcgccgatg cagcgatcgg acgggccacc
     3781 ggcgcgggct gcctctgcag ttcctgacgc ttctgggcga gctgccgctg cacctcctgc
     3841 tggatcgccc tggagtcgag cgcggcggcg ggaatcaccg cgggcgtgcc gaccgggacc
     3901 ggtggaggcg agacctcgag ag
//
LOCUS       DASWAV010000528         1916 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U2665
            k141_510287, whole genome shotgun sequence.
ACCESSION   DASWAV010000528 DASWAV010000000
VERSION     DASWAV010000528.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960316
            Sequence Read Archive: SRR10260007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 1916)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1916)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:47:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,929
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1916
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_510287"
                     /isolate="SMAG_U2665"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.36444 N 132.31889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..346)
                     /locus_tag="VF958_11080"
     CDS             complement(<1..346)
                     /locus_tag="VF958_11080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HEX9149693.1"
                     /translation="MLARARSHSWKTEGERDALIEQISAIRNLDAEDVAWLVVDLDDE
                     LRIRGKSLLGRFTYEEAQHALFPFLVARNDHIRRLAIDSLAAHAGPSFLDRLPELLGH
                     SDAALVHVSLEWL"
     gene            450..1022
                     /locus_tag="VF958_11085"
     CDS             450..1022
                     /locus_tag="VF958_11085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9149694.1"
                     /translation="MSSDRPCSPEVRRLRPGIVGDSFVEVVVCLALAAVVFLGIATLL
                     LSSRRTNDAADKYRIANGLVRDRLEQLMSLRFEDPRLSAGFHPDDLPATMPDPATGEY
                     PSSIPSIFRRSYRVSQFVLPPGESIAAGSPFPAVPVTEAGLRYDYKRIDVTVELSVTR
                     PGFGLIGARVSGIRCNPAPEEILSEASAGP"
     gene            1019..>1916
                     /locus_tag="VF958_11090"
     CDS             1019..>1916
                     /locus_tag="VF958_11090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9149695.1"
                     /translation="MRAPRERRRSAGFTLLEGIVAAFLGAAVLLGLLAVLDSAGRLNK
                     SEIAAAEAQGSLRYALNRITGVVRTAGAGGLFVTQSVLVRPDPALGGIAAGTDGDFDN
                     VAGGAVTNLSGGNVPVRPGTDVLEVRGVIFSPLVGFDDASGCGSCTGMAELTVRALTA
                     RNHVNDDAARRPQFSEIDAYTLAVSPEKPMFVVTAAADDLHAGCPPPAAGRTLPPQAS
                     YVVGKLTSPTALVARGTFGWIDFGDAAAREFATENPDDPAPAGTAAGLGPVTRAGVLD
                     DVLFFIDDSDPLHPVLAQGVRRG"
BASE COUNT          295 a          630 c          656 g          335 t
ORIGIN      
        1 gcagccactc gagcgagacg tggacgagtg cggcgtccga atgtccgagg agctccggca
       61 agcgatcgag gaacgagggg cccgcgtggg ccgcgaggga atcgatcgcg agccgccgaa
      121 tgtggtcgtt tcgggcgacg aggaatggaa agagggcgtg ctgggcctcc tcgtaggtaa
      181 agcggcccag caggctcttg ccccggatcc gcagctcgtc gtccaggtcc acgacgagcc
      241 acgcgacgtc ttccgcgtcg aggttgcgga tcgccgaaat ctgctcgatc agagcgtcgc
      301 gctcgccctc ggtcttccag gagtgagagc gcgcccgcgc gagcatggat cgagcatccg
      361 ctgtcggtcc acggcgcttt cttggccaca tttgatgtag gggaaagttt agccgagccc
      421 gcccggccgc gccgcgacaa ttctgagtaa tgagctcgga tcgcccctgc tcgcccgagg
      481 tcagacggct ccggccgggg attgtcggcg actcgttcgt ggaggtcgtc gtctgtctgg
      541 cgctggccgc ggtggttttc ctcggcatcg ccacgctcct cctctcgagt cgaaggacaa
      601 acgacgccgc ggacaagtac cgcatcgcga acggcttggt ccgggaccgg ctggaacagc
      661 tgatgagcct gcgcttcgag gacccgcggc tttctgcggg atttcacccc gatgatcttc
      721 cggcgacgat gcccgacccg gcgacgggcg aatacccgtc gtccatcccc agcatctttc
      781 gtcgaagcta tcgggtcagt cagttcgttc tgccgccagg agaatcgatt gcagccgggt
      841 cgcccttccc ggctgtcccc gtgacggaag cgggacttcg ctacgactac aagcgcatcg
      901 acgtcaccgt ggagctctcg gtgacgcgtc cggggttcgg actgatcggc gctcgagtga
      961 gcgggattcg gtgcaacccc gctccggagg agatcttgtc ggaggcgtcg gcgggtcctt
     1021 gagagctcca cgggagagac ggcgatccgc gggtttcacc cttctcgaag ggatcgtcgc
     1081 cgcttttctc ggcgcagctg tgctgctcgg cctcctcgca gttctcgatt ccgccgggcg
     1141 cctgaacaag agcgagatcg cggcggcgga ggcgcagggg agcttgcgct atgcgttgaa
     1201 tcgaatcacg ggcgtcgttc gtaccgcggg cgccggaggt ctgttcgtca cgcaatcggt
     1261 cctcgtgcgg ccggatccag cgctcggcgg aattgccgcc ggcacagacg gcgacttcga
     1321 caacgtcgcg ggcggagccg tgacgaatct ttcgggcggg aacgttccgg tgcggccggg
     1381 aacggatgta ctcgaggtgc gcggcgtgat cttctccccg ctcgtcggct tcgacgatgc
     1441 cagcggctgc ggctcgtgca ccggaatggc cgagctcacg gtgcgcgcgc tcacggcccg
     1501 caatcacgtc aatgacgacg ccgcaaggcg gccccaattc tcggagatcg atgcgtacac
     1561 actggcggtg tcacccgaga agccgatgtt cgtcgtcacc gcagcggccg acgatctcca
     1621 cgctgggtgt ccgccgccgg cagcgggccg cacgctgcct ccacaggcga gctacgtcgt
     1681 cggaaagctc acttcgccca cggcgctcgt cgcccgcggc acgttcggct ggatcgattt
     1741 cggagacgcc gccgctcggg agttcgcgac ggaaaacccg gatgatccgg cgcctgccgg
     1801 taccgcggcg ggtctcgggc cggtcacccg cgcaggcgtc ctcgacgacg tcctcttttt
     1861 catcgacgac tccgatccgc tgcaccccgt actcgcgcaa ggcgtgcggc gagggg
//
LOCUS       DASWAV010000529         3853 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U2665
            k141_348898, whole genome shotgun sequence.
ACCESSION   DASWAV010000529 DASWAV010000000
VERSION     DASWAV010000529.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960316
            Sequence Read Archive: SRR10260007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 3853)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3853)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:47:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,929
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3853
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_348898"
                     /isolate="SMAG_U2665"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.36444 N 132.31889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..589)
                     /locus_tag="VF958_11095"
     CDS             complement(<1..589)
                     /locus_tag="VF958_11095"
                     /inference="COORDINATES: protein motif:HMM:NF013126.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 N-terminal
                     domain-containing protein"
                     /protein_id="HEX9149696.1"
                     /translation="MRRPAAAAAPLLAFALACATGALRAPSAMTVDEKIGQLFVYPAT
                     GRFMNEASPEYGALVHQVRDNHVGGVLWFLLSDVYETARLNRRLQSLAAQPLLVAADL
                     EAGIGMRFQDTTNWPWPMAVAATGDPSLAEREGRVVAEEARAIGVSQIYAPVADVNVD
                     PANPTINVRSYGEDPETVGRFVSAFVRGVQSAGVLA"
     gene            complement(586..2046)
                     /locus_tag="VF958_11100"
     CDS             complement(586..2046)
                     /locus_tag="VF958_11100"
                     /inference="COORDINATES: protein motif:HMM:TIGR00813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter"
                     /protein_id="HEX9149697.1"
                     /translation="MRLLDIAIVFGYLAAVVAAGVFFARRQKTTAQYFLGGRHVPWWA
                     ISASIVATETSTITFISVPGLVYRDGGDFAFLQLVFGYLLGRVVISLLLIPSYFRREL
                     LTVYQLLQTRFGGSVKALAAGLFVAMRTIADGVRLLLTAFVLAAVYAAYAGAASADAV
                     VVGSILVIGIVMILFTFFGGMEAVIWIEVVQLGIYVTGAVAAGAVLIGKIPGGWEAAR
                     ALGEEYGKFRLFHFEWDFTKPFTFWAGLIGGCFLTMSTHGTDQYLVQRYLCTDKPRKA
                     TAALLTSGVVVLLQFTGFLLIGLLLFAFYRPFEHGAPLAGDRVFPDFITRHLPSGLSG
                     LVVAAIFAAAMSSSLNSIAATAVSDLYRPLAPDRSDRHYLAVSRWLTLAAGVAQIAVA
                     VGIRHMTRSALDAALSVASILNGPVLGVFLLGAFSKRAGTSAAFAGMAAGLVAVICVW
                     RLTPVAWPWYTLAGSMTTVAVGSALGLAGSRREAAA"
     gene            complement(2081..3082)
                     /locus_tag="VF958_11105"
     CDS             complement(2081..3082)
                     /locus_tag="VF958_11105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011683361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="slipin family protein"
                     /protein_id="HEX9149698.1"
                     /translation="MVPIVFSARKKGEKEPGEAERGKTPQHREREREADANVRRHVNG
                     VSLAAMILAVLAGGLAAFALRNRPAAAVLVSIASIAVGILLLFGLKIARQWERAVVLR
                     FGKFAGLRGPGLFAIFPIVETVSHLIDQRVRVTDVTAESALTRDTVPVNVDAIVFWTV
                     WDAEKSVLEVADYFDAVSLSAQTALREAIGRHQLAEMITEREKLGKELQAILDEKTNP
                     WGITVHSVEIRDVKIPPDLEDAMSRQAQAERERQARIILGTAETEIAARFVRAGEEYE
                     NRPVALHLRAMNMLYEAIKEKGSMVIVPSSAVETMGLGGLAGLTALGREPKTEEPKG"
     gene            complement(3089..3481)
                     /locus_tag="VF958_11110"
     CDS             complement(3089..3481)
                     /locus_tag="VF958_11110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013569603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEX9149699.1"
                     /translation="MTKLFRLDASSGVPFYRQIIDQVLLAVADGRLKPGAQLPTVRQL
                     AVDLSVNLNTVAKAYREMEIRGIVETQQGTGTFVATRRADRRPAERRRALEGLVDRFV
                     ALAASGGFSLEELAESVVERAEKSSARR"
BASE COUNT          701 a         1319 c         1256 g          577 t
ORIGIN      
        1 tggcgagcac tcccgccgac tggacgccgc ggacgaatgc cgagacgaac cgccctaccg
       61 tttccggatc ttcgccgtag ctgcggacgt tgatggtcgg gttggccgga tcgacgttga
      121 cgtccgcgac cggcgcataa atctggctca cgccgatcgc gcgggcttcc tcggcgacga
      181 cccgtccctc gcgctccgcg agagaagggt ccccggtggc cgcgaccgcc atcggccacg
      241 gccagttcgt cgtgtcctgg aaacgcattc caatgcccgc ctcgaggtcg gcggcgacga
      301 ggagcggctg cgcggcgagc gactggaggc ggcggttcag gcgtgcggtc tcgtaaacgt
      361 cggacaggag aaaccagaga accccaccga cgtggttgtc gcggacctgg tgcacgagcg
      421 caccgtattc gggcgacgcc tcgttcatga agcggccggt cgccggataa acgaagagct
      481 ggccgatctt ctcgtcaacg gtcatcgccg acggcgcacg cagcgcaccc gtcgcgcagg
      541 cgagagcaaa cgcgaggagg ggtgctgccg ccgcggccgg ccttctcatg ccgccgcctc
      601 gcgccggctg ccggcgagcc cgagcgccga tccgacggcg acggtcgtca tgctccccgc
      661 gagcgtgtac cacggccacg cgacaggcgt cagcctccac acgcagatga ccgcgacgag
      721 accggccgcc atccccgcga acgccgccga cgtcccggcg cgcttcgaga aagcgccgag
      781 aaggaacacc ccgaggaccg gaccgttcag gatcgacgcg accgagagcg cggcgtcgag
      841 cgcggagcgg gtcatgtgtc gaatgccgac ggcgaccgcg atctgcgcga ccccggcggc
      901 gagcgtcagc cagcgcgaca cggcgaggta gtgccggtcg ctccggtcgg gggcgagcgg
      961 ccgatagagg tccgacaccg cggtcgccgc gatcgagttc aacgaagaag acatcgcggc
     1021 cgcgaagatc gcggcaacga cgagcccgga aaggcccgac ggcaggtgcc gggtgatgaa
     1081 gtccggaaag acgcggtcgc cggcgagggg agcgccgtgt tcgaacggac gatagaaggc
     1141 aaagaggagg agcccgatca ggagaaaccc cgtgaactgc agcagcacga ccacgcccga
     1201 cgtgaggagt gcggccgtcg ccttgcgggg tttgtcggtg cagagatacc gctgaaccag
     1261 gtactggtcg gtcccgtggg tgctcatcgt gagaaaacag cccccgatca gtcccgccca
     1321 gaaggtgaac ggcttcgtga agtcccactc gaagtggaac agccggaatt tgccgtactc
     1381 ctcgccgagc gcgcgcgccg cttcccagcc gcccgggatc ttgccgatca gcacggcccc
     1441 ggcggcgacc gcgcccgtga cgtaaatgcc cagctggacg acctcgatcc agatcaccgc
     1501 ctccatgccg ccgaagaacg tgaagaggat catgacgata ccgatcacga ggatcgaccc
     1561 caccacgacc gcgtcggcgc tcgcggcccc cgcgtacgcc gcgtacacag cggcgaggac
     1621 gaatgcggtc agcaacagcc ggaccccgtc cgcgatcgtt ctcatcgcga cgaagagtcc
     1681 cgccgcgagc gccttcaccg agccgccgaa ccgcgtctgc aggagctggt agacggtcag
     1741 gagctcccgg cggaaatagg acgggatcag caggagggag atgacgaccc ggccgaggag
     1801 gtagccgaag acgagctgca gaaacgcgaa gtcaccgccg tcgcggtaga cgagtcccgg
     1861 tacggagatg aaggtgatcg tcgacgtctc ggtcgccacg atcgaggcgg agatcgccca
     1921 ccagggaacg tggcggccgc ccaggaaata ctgggccgtc gttttctgtc gccgggcgaa
     1981 gaagaccccg gcggcgacga ccgccgccag gtacccgaag acgatcgcga tgtcgaggag
     2041 gcgcaaaccg gggaaaacct atcagcatcc ccgccggcgg tcagcccttc ggttcctccg
     2101 ttttcggttc gcgcccgagc gccgtcaggc cggcgaggcc gccgagcccc atcgtctcga
     2161 ccgcgctcga gggaacgatc accatcgacc ccttttcctt gatcgcctca tagagcatgt
     2221 tcatcgcgcg caggtgcagc gcgaccggtc ggttctcgta ctcctccccc gccctgacga
     2281 aacgcgctgc gatctccgtc tccgccgttc cgaggatgat tcgggcctgg cgctcgcgtt
     2341 ccgcctgcgc ctgccggctc atcgcgtctt cgagatccgg gggaatcttc acgtcgcgga
     2401 tctcgacgga gtgcacggtg attccccacg gattcgtctt ctcgtcgagg atcgcctgaa
     2461 gttcctttcc gagcttctcc cgctcggtga tcatctccgc gagctggtgc cgtccgatcg
     2521 cctcccgcag cgcggtctgg gccgaaaggc tcaccgcgtc gaaatagtcg gcgacctcca
     2581 ggacggattt ctccgcgtcc cacaccgtcc agaagacgat ggcgtccacg ttgaccggaa
     2641 cggtgtcgcg cgtcagcgcg gactccgccg tcacgtcggt gacgcgaacg cgctggtcga
     2701 tcaggtgtga caccgtttcg acgatcggga agatcgcgaa gaggccgggg ccgcgcagcc
     2761 cggcgaactt tccgaaacgc aggacgacgg cgcgctccca ttgccgcgcg atcttcagcc
     2821 cgaagagcag caggattccg acggcgatcg aggcgatcga gacgagaacg gcggcggccg
     2881 gccggtttcg cagggcgaac gccgcgagcc caccggcgag caccgcgaga atcatcgcgg
     2941 cgagactcac gccgttgacg tggcgccgga cgttcgcatc ggcttcgcgc tcccgctccc
     3001 ggtgctgcgg ggtcttccct cgctcggcct ctccgggctc cttctccccc ttttttcgtg
     3061 ccgaaaacac gatcgggacc atgttctcct acctccttgc gctcgatttc tcggcgcgtt
     3121 ccacgacgga ctcggcgagc tcttccagcg agaaaccgcc cgatgcggcg agcgcgacga
     3181 agcggtccac gagaccctcg agagccctcc gccgctccgc cggccgccgg tcggcccggc
     3241 gagtcgccac gaacgttccc gtgccctgct gggtctcgac gattccccgg atctccatct
     3301 cccggtatgc cttggcgacc gtattcaggt tgaccgacag gtcgaccgcg agctgccgca
     3361 cggtcggcag ctgcgcgccc ggcttcagcc gcccgtccgc gaccgcgagc agcacctggt
     3421 cgatgatctg ccggtagaac ggcactcccg aggacgcgtc gagccggaag agctttgtca
     3481 tagtcaacta gtacaatagg tattgtcggg ccaccggggt gcgatgtcaa gcctcccgca
     3541 cggcagcgcc ttcgcatcgg accactacga cgatggtcga agggaaaaac ggccctctcg
     3601 tccaaaaaaa tgtacggaag ccggtcagaa aaaaactctg cacactaaat tgtgcagaga
     3661 ctagcaaccg cgacggaagt tcggataagt tcccgccgac aacccaattc gtgaagacat
     3721 tttcccagtt cgaaactgtg aataaggagg aatccatgag gcgacctctg ctccggcgat
     3781 acgcctgggc gctttccgtt tcggtcgcgg tgctcgcgtt cgccgttgca ccgccggcgt
     3841 tcgcgcagac tga
//
LOCUS       DASWAV010000530         2146 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U2665
            k141_387366, whole genome shotgun sequence.
ACCESSION   DASWAV010000530 DASWAV010000000
VERSION     DASWAV010000530.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960316
            Sequence Read Archive: SRR10260007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2146)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2146)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:47:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,929
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2146
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_387366"
                     /isolate="SMAG_U2665"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.36444 N 132.31889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1151)
                     /locus_tag="VF958_11115"
     CDS             complement(<1..1151)
                     /locus_tag="VF958_11115"
                     /inference="COORDINATES: protein motif:HMM:NF015859.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHASE domain-containing protein"
                     /protein_id="HEX9149700.1"
                     /translation="MRSALSSWRASIRAADVLVASVVAALSLGLGAWIFSRAQAATHL
                     AFTQRAQLLQAAVTERLVLPQEDLATLASFLEASGGTTTRRQFRVLVEPMLKRRRLVY
                     AYEWLPVVRESERPAYEAEARAAGLSDYRFWELAGGEQRTAGSRAFYVPIHYMEPPSV
                     LALGFDIAASPLRWDTAQMARDAGRVAVSHPFTLIEDASKADALPVVAMYYPVYKEGD
                     PGSETARRAALTGFGMAIFRVAPVIGDASAAVDASGLGLLLRDPEAKETPILVERPRD
                     ATKLPRRAGYDLEFPVPFADRRWMLTVFPLPGAFLPPKRGAIGIALAGVLAALLGIAT
                     VTSLRTISRLRRQVEKVGPYRLVARLGHGAMGVVWEARHALLRRPTAVK"
     gene            complement(1318..>2146)
                     /locus_tag="VF958_11120"
     CDS             complement(1318..>2146)
                     /locus_tag="VF958_11120"
                     /inference="COORDINATES: protein motif:HMM:TIGR03118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TIGR03118 family protein"
                     /protein_id="HEX9149701.1"
                     /translation="TGSHSSTLYTGDGVKLSLEVSVPGAPTGMVFNSGTSFPLANSVK
                     ASFIWATEDGRFFGWNNGSGTAAEQIFSDPGSSYKGLAIHGDTLFSTDFGECKVEAFE
                     FINDAVMEVETTGDFEDETIPGNYCPFGIQAVGDSIFVTYAVKGGEDDVAGQGHGLVR
                     EFDTDGNLVAPVADHGQLNSPWGIALAPANFGRFGGCLLVGNFGDGRINAYCKDDEGE
                     WHHGGALREGKRPLVIDGLWGIGFGNDHSSGPSTTLYFAAGPDEETNGYFGKVEAVQ"
BASE COUNT          371 a          734 c          716 g          325 t
ORIGIN      
        1 aacttgacgg cggtcggccg ccgcaggaga gcatgccgcg cctcccacac gacccccatc
       61 gcgccgtggc cgaggcgcgc caccagccga tagggcccga ccttctccac ctggcggcgc
      121 aaccgcgaga tcgtccggag cgacgtgacc gtagcgatgc cgagcagcgc ggcgagcact
      181 ccggccaggg cgatcccgat cgccccccgt ttcggcggga gaaaggcgcc gggaagcgga
      241 aagacggtca acatccagcg gcgatcggcg aacggcaccg ggaattcgag gtcgtatccc
      301 gcgcgacggg gcaacttcgt cgcgtcgcgc ggccgctcca ccaggatggg cgtctccttc
      361 gcctccgggt cccgcagcag gaggccgagt ccggaagcgt ccaccgctgc ggacgcgtct
      421 ccgatcaccg gtgcgacccg gaagatcgcc atcccgaaac ccgtgagcgc ggcgcggcgc
      481 gccgtctccg agccggggtc accctctttg taaacggggt agtacatcgc gacgaccggc
      541 agggcgtcgg ccttgctcgc atcctcgatc agcgtgaacg ggtgcgaaac cgcgacgcgg
      601 ccggcgtcgc gcgccatctg ggccgtgtcc cagcggagag gggaagcagc gatgtcgaaa
      661 ccgagcgcca ggacgctcgg gggctccatg taatggatcg gaacgtagaa agcgcgactg
      721 ccggccgtgc gctgctctcc cccggcgagc tcccagaatc gatagtctga aagaccggcg
      781 gcgcgcgctt ccgcctcgta ggccgggcgc tcggactctc gaacgaccgg gagccactcg
      841 taggcgtaga cgagccggcg gcgtttcaac atcggctcga ccagcacgcg gaactgacgg
      901 cgcgtcgtgg ttccgcccga ggcttcgagg aagctcgcga gcgtcgcgag atcttcctgc
      961 gggaggacca gtctctccgt gaccgccgcc tgaaggagct gcgcccgctg agtgaacgcg
     1021 aggtgggtcg ccgcctgggc gcgagaaaag atccaggcgc cgagcccgag cgacagggcc
     1081 gcgaccacgc tcgcgacgag gacgtccgcg gcgcggatcg aggcgcgcca ggaggagaga
     1141 gcggaccgca cgcggcgccc cgtgaatcga cccgtctccg aactggaccg gctcccgcga
     1201 gagcgcggcg ggattgtcac gccccgatta tcttgcggag ggactttttc gggcgaagga
     1261 aagggcggac aaagcgtccg cccttttccc gaattcctga cccttcgacg aaaaacccta
     1321 ctgcacggcc tcgaccttgc cgaaataacc gttcgtctcc tcgtccggcc cggccgcgaa
     1381 gtagagagtg gtgctcggac cggagctgtg atcgttgccg aagccgatgc cccacagccc
     1441 gtcgatgacg aggggacgct tgccctcacg cagggcgccg ccgtgatgcc attccccttc
     1501 gtcgtccttg cagtaggcat tgatccggcc gtcgccgaaa ttgccgacga gcaggcatcc
     1561 gccgaaacgg ccgaagttcg ccggagcgag cgcgatgccc cagggggagt tcaactgacc
     1621 gtggtcggcc accggggcga ccaggttgcc gtcggtgtcg aactcgcgca ccaggccgtg
     1681 cccctggcct gcgacgtcgt cctcgccccc tttcaccgcg taggtgacga agatcgagtc
     1741 gccgaccgcc tggattccga acgggcagta gttcccggga atggtctcgt cttcgaaatc
     1801 tcctgtggtc tcgacttcca tgacggcgtc attgatgaac tcgaacgctt ccaccttgca
     1861 ctcgccgaag tcggtgctga agagagtgtc gccgtggatc gccagtccct tgtacgacga
     1921 tcccggatcg ctgaagatct gctccgccgc cgtgccgctg ccgttgttcc agccgaaaaa
     1981 ccggccgtcc tcggttgccc agatgaaact tgctttcacg gagttcgcga gcgggaacga
     2041 cgtcccgctg ttgaagacca tgcctgtcgg cgctccggga accgagacct ccaggctgag
     2101 cttcacgccg tcgccggtgt acaacgtcga cgaatgcgag ccggtg
//
LOCUS       DASWAV010000531         1566 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U2665
            k141_789062, whole genome shotgun sequence.
ACCESSION   DASWAV010000531 DASWAV010000000
VERSION     DASWAV010000531.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960316
            Sequence Read Archive: SRR10260007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 1566)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1566)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:47:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,929
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1566
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_789062"
                     /isolate="SMAG_U2665"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.36444 N 132.31889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..664)
                     /locus_tag="VF958_11125"
     CDS             complement(<1..664)
                     /locus_tag="VF958_11125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020721417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006614 - SRP-dependent cotranslational
                     protein targeting to membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle receptor subunit
                     alpha"
                     /protein_id="HEX9149702.1"
                     /translation="MSETRDAPRRGGFFSKVRRGLFMTHTEILEKLGEAVKKGLGFDE
                     SVLQSLEDALLEADVGAETAAALTEAVRRRAGSAQRGDLPELRAMVISEIEGVLARAP
                     RPAASAEVPLEVVFLVGVNGSGKTTTTAKLAAREVAQGRTVLLAAADTFRAGAIEQLE
                     IWAGRLGLPIVRHKEGADPSAVLFDALTAAKARKIEKVFVDTAGRLHTKKNLMEELAK
                     MRR"
     gene            complement(714..1475)
                     /locus_tag="VF958_11130"
     CDS             complement(714..1475)
                     /locus_tag="VF958_11130"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX9149703.1"
                     /translation="MTALRRGLVPAAAALLFALTACSASKKTGSAEVLLNERMGAVLL
                     REGRAVEAERAFREVLPHDSKNPEVHDGLGVSLLMQGRFKEAYSCFDRAVDIDPEKPL
                     FRIHRGMAGTELGRFKEAEGDFDRAEASPAAADRLDAAINRGRLKQRQKDFAAAEEAF
                     SSALSREPKSSAALIGRGIARESRGAMEQAAEDYLEAIRVDPKNAEAHLRLGLALVTL
                     KKTALGRRYLERTIELDPTGETGARARLLLDNSKS"
BASE COUNT          233 a          547 c          523 g          263 t
ORIGIN      
        1 tccggcgcat cttcgcgagc tcctccatga ggtttttctt cgtgtgcagg cgcccggcag
       61 tgtcgacgaa gaccttctcg atcttccggg cctttgccgc cgtgagggcg tcgaagagta
      121 ctgcggacgg atcggcgccc tccttgtggc gaacgatcgg taacccgagg cggccggccc
      181 agatctcgag ctgctcgatt gccccggcgc gaaaagtgtc ggcggcggcg agaagtacgg
      241 tacgaccctg cgccacctcc cgggccgcga gcttcgccgt cgtggtcgtt tttcccgagc
      301 cgttgacgcc gaccaggaag acgacttcga gaggcacctc ggcggaagcg gccgggcgcg
      361 gcgcgcgggc cagaacgcct tcgatctcgg aaatcaccat cgcacgaagc tcggggaggt
      421 cgcctcgttg cgccgacccg gcccgccgcc ggacggcctc cgtgagcgct gccgccgtct
      481 ccgcgccgac gtccgcctcg agaagggcgt cctcgagcga ttggaggacc gactcgtcga
      541 acccgagccc cttcttcacc gcctcgccga gcttctcgag gatctcggtg tgggtcatga
      601 agagtccccg gcgcaccttg gagaagaagc cgccgcggcg cggggcgtcc cgtgtctcgc
      661 tcatctcaag ggattctatg ggcgggaagt cgcgcgccgg cgcgagagag ttctcaggac
      721 ttggagttgt ccaggagcag gcgcgctctc gccccggttt ctcccgtcgg atcgagctcg
      781 atcgtccgtt cgaggtaacg ccggcccagc gccgtctttt tgagcgtcac gagcgccagt
      841 ccgaggcgga ggtgcgcctc cgcgttcttt ggatcgaccc ggatggcttc gaggtaatcc
      901 tccgctgcct gctccatggc gccgcgcgat tctcgcgcga tcccgcggcc gatcaacgcc
      961 gccgacgact tcggctcgcg ggagagggcg ctcgagaacg cctcctccgc cgcggcgaaa
     1021 tccttctgcc gctgtttcaa ccggccgcgg ttgatcgcgg cgtccagccg atccgcggcc
     1081 gcgggggaag cctccgccct gtcgaagtct ccctcggcct ccttgaaccg tccgagctcg
     1141 gtcccggcca tgccgcggtg aatgcgaaag agcggcttct ccggatcgat gtcgacggcg
     1201 cgatcgaagc aggagtaggc ctccttgaac ctgccctgca tgagcagcga gacgcccaag
     1261 ccgtcgtgga cctcggggtt cttcgaatcg tgcgggagga cctcgcggaa ggcacgctcg
     1321 gcctcgacgg cccgcccttc acggagcaga accgcgccca tgcgctcgtt caagaggact
     1381 tccgccgagc ccgtcttctt tgaagccgag cacgcggtca gggcaaaaag cagcgcggcg
     1441 gccgccggga cgagaccgcg ccgcagagcg gtcaactcgc ctcgacgcgc cggagcgggg
     1501 gcgcctcgtg cggaatctct tcccgcgcaa tcgggacgag gtcccccttc tcgtacgagt
     1561 ggatca
//
LOCUS       DASWAV010000532         2356 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U2665
            k141_348955, whole genome shotgun sequence.
ACCESSION   DASWAV010000532 DASWAV010000000
VERSION     DASWAV010000532.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960316
            Sequence Read Archive: SRR10260007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2356)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2356)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:47:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,929
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2356
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_348955"
                     /isolate="SMAG_U2665"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.36444 N 132.31889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(7..1302)
                     /locus_tag="VF958_11135"
     CDS             complement(7..1302)
                     /locus_tag="VF958_11135"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HEX9149704.1"
                     /translation="MDCRVDLSLDLRQNYAPVETEEKIRLLMEQALSDREGDGPLRDL
                     AAGGRTREPLDPINIRSLPDRGHGPTRLFRILLTNACSFSCDYCPMRASRVIPRHALA
                     PQSLADAFLTAYRRRWVSGLFVTTGIPRNPRWAMDRLIELVEILRLRRGYAGYLHVKA
                     VSGAEPEQVDRLVELCDRVSYNLEAACQATLDSVAREKSFDEGLSLLERVRRRAVELG
                     RQRVKGDPRPEGSANAAGMTAQFVVGIGKEADRDFLAATHRLWRQRLLHHPHFAAFRP
                     IEDTPLENERETPLLRENRLYQADYLLRRYGFEPGELVYRGDGNLPLSRDPKLAWALA
                     HPERFPVEASRADRETLLRVPGLGPKTVERLLAGRRTLARLDLAGLRRLGAACARAAG
                     FLSWRGKTLGTREFQESLFPPEDFPEPSRVYGFSPGTFR"
     gene            complement(1320..>2356)
                     /locus_tag="VF958_11140"
     CDS             complement(1320..>2356)
                     /locus_tag="VF958_11140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9149705.1"
                     /translation="LLIDRASPGHFLPVGQGIGLARKIGCDLMLGLAASGRLASPWIH
                     ATDADVRLPNDYFEQIGRLSLERTSAAVYSFEHRFPPEEDLARAGRLFEISLRYNVLG
                     LAWAGSPYAYQSLGSCLAIAPWAYAEVGGFPRVNVIEDVKIANDLAKIGSIERLAGAP
                     IGLEGRISARVPVSTGQSLSKLVAKKGAAAGFELHHPIVFAHLAAWLRVLGAIARRGS
                     DLEAPLDALPRGNPFFRADLLEESLGQMGAFEAVREAIAQPGDEETVLRRLHVWFDAF
                     RTRRLMDDLRDGGLVSLPLRKALSEAPFTGLGDSTDDDLETLRLLLAEEEKTLALVPA
                     GVPALEPTRA"
BASE COUNT          387 a          779 c          852 g          338 t
ORIGIN      
        1 gcgcgactac cggaacgttc cgggcgagaa gccgtagacc cgggacggct cggggaagtc
       61 ctcgggtggg aagagggact cctggaactc ccgcgtcccc agggtctttc cccgccacga
      121 aaggaatccc gccgcgcgtg cgcacgccgc gccgagcctg cgcagcccgg cgaggtcgag
      181 ccgtgcgagc gtgcgccgcc cggcgagcag cctctcgacg gtcttcggac cgaggcccgg
      241 gacccgcaga agcgtctctc gatccgcgcg ggaagcttcg acggggaagc gctccgggtg
      301 cgcgagcgcc caggcgagct tcgggtcacg cgagagcggg aggttgccgt cgccgcgata
      361 gacgagctcc ccgggctcga acccgtagcg gcgcaggagg tagtcggcct ggtagaggcg
      421 gttctcacgc agcagggggg tctcgcgctc gttctcgagg ggagtgtcct cgatcggtcg
      481 gaacgccgcg aagtgcgggt ggtgcagcag ccgttggcgc cagagacgat gggtcgcggc
      541 gaggaagtcc cgatccgctt ccttccctat gccgaccacg aactgcgccg tcatcccggc
      601 cgcgttcgcg cttccttccg gccggggatc tcccttgacg cgctggcggc cgagctcgac
      661 ggcgcggcgg cggacgcgct cgagcagcga cagcccctcg tcgaagctct tctcgcgcgc
      721 gacgctgtcg agcgtcgcct ggcaggcagc ctcgaggttg taagaaacgc ggtcgcagag
      781 ctccaccagg cgatcgacct gctccggttc cgcgcccgac accgccttga cgtggagata
      841 ccccgcgtat ccgcggcgca gccgcaggat ttcgacgagc tcgatcagac gatccatcgc
      901 ccagcgcggg ttccggggga ttccggtggt cacgaacagg ccggagaccc agcggcgccg
      961 ataggcggtc aggaaggcgt cggcgagcga ctggggggcg agggcgtggc gagggatcac
     1021 gcgcgaagcg cgcatcgggc agtagtcgca ggagaaggag cacgcgttgg tgagaagaat
     1081 tcggaagagc cgcgtcggac cgtgcccgcg atccgggagc gagcggatgt tgatggggtc
     1141 gagcggctcg cgcgtgcgcc cgccggccgc aaggtcccgg aggggaccgt ctccttcgcg
     1201 gtccgagagc gcctgctcca tgaggaggcg gatcttttcc tcggtttcca ccggggcgta
     1261 attctggcgc aagtccagcg aaaggtcaac ccggcagtcc acgtctctcc gagcccctcc
     1321 taagcccggg tcggctcgag cgccggaacg cccgcgggga ccagggcgag cgttttctcc
     1381 tcttcggcca ggagcaaacg aagcgtttcc aagtcgtcgt cggtcgagtc gccgaggccg
     1441 gtgaagggcg cttcggagag cgcctttcgc aacggtagcg agacgagccc gccgtctctc
     1501 aagtcgtcca tgagccggcg cgttcggaaa gcgtcgaacc agacgtggag cctacgcagc
     1561 acggtctctt cgtcgcccgg ctgcgcgatg gcctcgcgca ccgcttcgaa agcgcccatc
     1621 tgcccgagcg actcttcgag gaggtcggcg cggaagaaag ggttcccgcg agggagagcg
     1681 tcgagcggcg cctcgaggtc gcttccgcgg cgcgcgatcg ctccgagcac gcgcagccac
     1741 gccgcgaggt gagcgaacac gatcggatgg tgaagctcga atccggcggc tgctcccttc
     1801 ttcgcgacga gcttcgacag tgactgcccg gtcgagaccg gaacgcgagc cgagatgcga
     1861 ccctcgaggc cgatcggcgc gcccgcgagt cgctcgatcg aaccgatctt ggcaaggtcg
     1921 tttgcgatct tcacgtcctc gatgacattc acgcgcggga agcccccgac ctccgcgtac
     1981 gcccacggcg cgatcgcgag gcacgatccc aggctctgat aggcataggg cgaacccgcc
     2041 catgccagtc cgagaacgtt gtagcgcagc gagatctcga agagccgtcc ggcgcgcgcg
     2101 aggtcttcct ccggcgggaa acggtgctcg aaagagtaga ccgccgccga ggtccgctcg
     2161 agactcagcc gaccgatctg ctcgaagtag tcgttcggga gccgcacgtc cgcgtcggtc
     2221 gcgtggatcc acggggaggc gagccgcccg gacgcagcga ggccgagcat gaggtcgcaa
     2281 ccgatctttc gggcgaggcc gattccctgt ccgacgggaa ggaagtggcc gggggaggcg
     2341 cggtcgatca ggagca
//
LOCUS       DASWAV010000533         3600 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U2665
            k141_310301, whole genome shotgun sequence.
ACCESSION   DASWAV010000533 DASWAV010000000
VERSION     DASWAV010000533.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960316
            Sequence Read Archive: SRR10260007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 3600)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3600)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:47:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,929
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3600
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_310301"
                     /isolate="SMAG_U2665"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.36444 N 132.31889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            152..2407
                     /locus_tag="VF958_11145"
     CDS             152..2407
                     /locus_tag="VF958_11145"
                     /inference="COORDINATES: protein motif:HMM:NF039587.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5916 domain-containing protein"
                     /protein_id="HEX9149706.1"
                     /translation="MAHHEGIRTWLVLGLFGAAITGSAAPPEKGTPIPIAKASGAIVV
                     DGDLSDPGWQGATRVTTWYETNPGDNTEPKFKNVGYLTYDDKFLYAGFEFSDDEPAKI
                     RAPYGDRDNVSSTTDYGGIILDTQGDARRAILFLANPHGIQYDANTDDASGEDSSPDY
                     FWDSAARVTSTGWVLELRVPFSSLRYEKSDSPNWNIMLYRNHPRDFRYQYFSTKLPRG
                     GNCFICRSNPLTGLTGLPSGGHLVVAPYATASQDAFPKDELGSPLENESVEADAGLDV
                     KWTPNVNTAIDATVNPDFSQIESDVAQIGANERFALFFPEKRPFFLEGIELFSTPIQA
                     VYTRTITSPRWGARGTGKIGRTAYTALVVDDRGGGSVILPGPNSSDLADQEFRSIAAV
                     ARIRHEVGQSFVSFLATDREIRDSDGGGYNRVFGPDFRWSLSEADTITGQLLFSSSQA
                     PRRTDLASEWNGQKLSGHAGQIWWSHSTRTFDFFTQYNDYGDEFRADNGFVPQVGFRE
                     NYGEAGWTFRPTGAVRRLRTYLIADYQAQQDGALIFRQVSPGFGMDALLNSFVRLRVG
                     FDKVRAGAKTFDRTQLLYEIEASPSRVFNRIGINGNIGQQVDFANSRRGDGATVVVFA
                     VVRPTDHLELRFDGSRRWLNVDAVNAKGRVFTASVARLRTQYTFNARAFLRVIGQYVE
                     TKRDPSLYIDAADRKDGDFSGTILLAYKLNWQSVLFIGYGDTRTFLEETDRLERASRQ
                     LFLKLSYAFQL"
     gene            complement(2411..>3600)
                     /locus_tag="VF958_11150"
     CDS             complement(2411..>3600)
                     /locus_tag="VF958_11150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9149707.1"
                     /translation="DEPTSPASRIEWEPVFQEADLDIRRFASTPPRWVPPTPYDSRAE
                     WDGTSVRHPETPIHVTAAGYRGKLVYFASAGPWDRPLRMELPFQGFNPVIGAVMFVVL
                     LTLLIAGVVLARRNLRLGRGDRRGALRIATYVYFVLMIAWLLRAHHVPNLAIEGDLFS
                     TGLGSALLAAAFVWLSYMALEPYVRRRWPDLLISWNRLLAGRFRDPLVGRDFLVGTLL
                     GATNAMLLHISNALPAWMDAPGMTPVPASDLMMRGTREAASFFFVRQNGAAFMALVFM
                     FLFFLAASIFRKKWLGAVPLGVLFTMIHLSGENVAIELPFAILMAAVLVFVVLRFGLL
                     SVAVAGVVAPLLTFSPLTLDLSRWYAGRSLFALAVVVGVALYGFRVALGKRPVFGAVA
                     LED"
BASE COUNT          705 a         1195 c         1153 g          547 t
ORIGIN      
        1 tcgcgcctcc gcgagaacag agactagaga ggtgcccgtg aatgcgccgt gaacgcccgg
       61 gttcggcggg aatcgccgaa tcgcgtgttc cgccccccgg gaggccctga aaaccccaaa
      121 ctttttcaaa aacggtacgt ctaaccagac ggtggcgcac cacgaaggta ttcgtacttg
      181 gctcgtcctc ggcctgttcg gcgccgccat caccgggtcc gcagccccgc ccgaaaaagg
      241 cactcccatt ccgatagcga aggcttccgg cgcgatcgtc gttgacggtg acctctcgga
      301 ccccggctgg cagggtgcga caagagtgac cacctggtac gaaacgaacc cgggagacaa
      361 cacggagccg aagttcaaga acgtcggcta cctgacgtac gacgacaaat tcctctacgc
      421 cggtttcgag ttctccgacg acgagccggc aaagattcgc gccccgtacg gggatcgcga
      481 caacgtgtcc agcacgaccg attacggcgg catcatcctc gacacgcagg gggacgcgcg
      541 gagggcgatc ctcttcctcg cgaaccccca cgggatccag tacgacgcga acaccgacga
      601 cgcgagcgga gaggacagct cgcccgacta cttctgggac tccgccgcgc gcgtgacctc
      661 gacgggctgg gttctcgagc tgcgcgttcc gttctcttcg ctccgctacg agaagtccga
      721 ctctccgaac tggaacatca tgctgtaccg gaaccacccg cgtgatttcc ggtaccagta
      781 cttctcgacg aaactccccc gcgggggaaa ctgtttcatc tgccgctcga atccgctcac
      841 cggcctgacc ggcctgccgt cgggcggaca ccttgtcgtc gcgccttacg cgaccgccag
      901 ccaggacgct ttcccgaagg acgagctcgg ttcgccactc gagaacgagt cggtcgaggc
      961 ggacgcggga ctcgacgtca agtggacccc caacgtcaac acggccatcg acgcgaccgt
     1021 gaacccggac ttctcgcaga tcgagtccga cgtcgcgcag atcggcgcga acgagcggtt
     1081 cgcgctcttc ttcccggaga agcgaccgtt cttcctggag ggaatcgaac tcttctcgac
     1141 gcccatccag gcggtctaca cgcgcacgat cacgtctccc cgctggggcg cgcgcgggac
     1201 cgggaagatc ggacgcacgg cctacacggc tctcgtcgtg gacgaccgtg gaggcgggag
     1261 cgtcatcctc cccggcccca attcctcgga cctcgccgac caggagttcc gctcgatcgc
     1321 ggccgtcgcg cgcatccgtc acgaagtcgg gcagtccttc gtcagcttcc tcgcgaccga
     1381 ccgcgagatc cgcgacagcg acggcggcgg ctacaaccgc gttttcggtc cggacttccg
     1441 ctggagcctc tcggaggcgg acacgatcac cgggcagctc ctgttctcga gcagccaggc
     1501 accgcgacgc acggatctcg cttcggagtg gaacgggcag aagctctcgg gccacgccgg
     1561 ccagatctgg tggtcgcact cgacgcgcac cttcgacttc ttcacccagt acaacgacta
     1621 cggcgacgag ttccgcgccg acaacggctt cgtgcctcag gtcgggttcc gcgagaacta
     1681 tggcgaggcc ggctggacgt tccggccgac cggcgcggtg cggcgcctgc ggacctatct
     1741 catcgccgac tatcaggcgc aacaggacgg cgcgctcatc ttccggcagg tctcgcccgg
     1801 gttcgggatg gatgcgctgc tcaactcgtt cgtccggctg agggtcgggt tcgacaaggt
     1861 gcgcgcgggg gcgaagactt tcgatcggac gcagctcctc tacgagatcg aggcgagtcc
     1921 gtcgcgggtt ttcaaccgga tcggtatcaa cggcaacatt ggtcagcagg tcgacttcgc
     1981 gaactcgcgc cggggcgacg gcgccaccgt cgtcgtgttc gccgtcgtcc gtccgacgga
     2041 ccacctcgag ctgcgcttcg acggaagccg tcgctggttg aacgtcgacg cggtgaacgc
     2101 gaaaggacgc gtcttcaccg cctccgtggc gcgcctgcgc acgcagtaca cgttcaacgc
     2161 ccgcgccttc ctgagggtga tcggccagta tgtcgagacg aagcgggacc cgtcgctcta
     2221 catcgacgcc gcggaccgga aagacggcga cttcagcgga acgattctcc tcgcctacaa
     2281 gctcaactgg cagtcggtgc tcttcatcgg atacggcgac acgcgcacgt tcctggagga
     2341 aacggaccgt ctggaacggg cgagccgcca gctcttcctg aagttgtcct acgccttcca
     2401 gctctaggcg tcagtcttcc agagcaaccg ccccaaaaac cggacgtttg cccagagcga
     2461 cgcggaaacc gtacagcgca acgccgacga cgaccgcgag ggcgaacagt gaccgaccgg
     2521 cgtaccaccg cgagaggtcg agggtgagcg gcgagaacgt caagagaggg gcgacgaccc
     2581 ccgcgaccgc caccgacagg agaccgaagc gaagcacgac gaagacgaga acggccgcca
     2641 tgaggatggc gaagggtagc tcgatcgcga cattctctcc gctcaggtga atcatggtga
     2701 agagaacacc gagcggcacc gccccgagcc acttcttccg aaaaatggag gctgcgagaa
     2761 aaaagaggaa catgaagacg agcgccatga aggcggcacc gttctgccgc acgaagaaga
     2821 agctggccgc ctcccgggtg ccccgcatca tcaggtcgga ggcaggaacg ggtgtcatcc
     2881 ccggagcgtc catccacgcg gggagcgcat tcgaaatgtg aaggagcatc gcgttcgtgg
     2941 cgccgaggag ggtcccaacg agaaagtctc ggcccaccag aggatcccgg aaccgtccgg
     3001 cgaggagtcg attccacgag atcaggagat cgggccagcg ccgccggacg tagggctcga
     3061 gagccatgta gctcaaccag acgaacgcgg ccgcgaggag cgcgcttccc agacccgtcg
     3121 agaatagatc tccttcaatc gccaggttcg ggacgtgatg cgctcgcaga agccaggcga
     3181 tcatcaagac gaaatagacg taggtcgcga tccgcagcgc tccccggcga tccccgcggc
     3241 cgagtcggag gttccgccgc gcgagcacga cgccggcgat caacagcgtg agaaggacga
     3301 cgaacatgac tgcgccgatc accggattga acccctggaa cggcagctcc atgcgaaggg
     3361 gacggtccca cggtccggcg gacgcgaagt agacgagctt gccgcgatag ccggcggcgg
     3421 tcacgtgaat cggcgtttcg gggtggcgca cggaagttcc gtcccactcg gcccggctgt
     3481 cgtacggcgt gggtggaacc catcggggcg gcgtcgaggc gaagcgtcgg atgtccaggt
     3541 ccgcctcttg gaagaccggt tcccactcga tccgggaggc aggtgaggtg ggttcgtcga
//
LOCUS       DASWAV010000534         7415 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U2665
            k141_668853, whole genome shotgun sequence.
ACCESSION   DASWAV010000534 DASWAV010000000
VERSION     DASWAV010000534.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960316
            Sequence Read Archive: SRR10260007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 7415)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7415)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:47:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,929
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7415
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_668853"
                     /isolate="SMAG_U2665"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.36444 N 132.31889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(116..1372)
                     /gene="hutI"
                     /locus_tag="VF958_11155"
     CDS             complement(116..1372)
                     /gene="hutI"
                     /locus_tag="VF958_11155"
                     /EC_number="3.5.2.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR01224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050480 - imidazolonepropionase activity
                     [Evidence IEA];
                     GO_process: GO:0006548 - histidine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazolonepropionase"
                     /protein_id="HEX9149708.1"
                     /translation="MTSLEIRGAACLATPLGRSARRGREQREIRRIRHPVVRMENGVL
                     EFVGTEADYRREYSGRPAGVTVDAAGGTLLPGFVDAHTHPVWEGDRGEEIGRRLAGES
                     YANIAAAGGGILATVRATRAASDDELSATVRRRLAAMLAHGTTTVEAKSGYGLTVEDE
                     LRSLRLLKALAEKPDLPAIVPTLLAAHEVPPEFRERRQEWVRIVADEIVPRAAREGLA
                     RFCDVFCEEGVFTVAESRTILEAARRVGLGLRVHADELARSGGTLLAAELGAASADHL
                     LFAGDDEIAALARSGIVAVILPGTAWWMRSRRAPARAMIAAGVPVAVASDANPGTCFT
                     ESLASVAAHACLDSGLTVEETLTGLTLNAAASLGLADRIGTLEPGKTADLVVLDAPDD
                     RHLVYHWGVNLVRTVVRGGLIVSSES"
     gene            complement(1491..1988)
                     /locus_tag="VF958_11160"
     CDS             complement(1491..1988)
                     /locus_tag="VF958_11160"
                     /inference="COORDINATES: protein motif:HMM:NF016959.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DivIVA domain-containing protein"
                     /protein_id="HEX9149709.1"
                     /translation="MSRLTPLEIQKQRFSRKWRGLDPTEVGAFLDMAAEEMETLAREN
                     AALGGRIRGLEEENAEHRERERILKDTLLSAQRASEDIRAAAQTKAELVVQEAEDSAE
                     RLTHNAMLRAAEVEKAIHELKLGRANFRLEIQKMLELFQQVLEMDSKQDEQDRPLSYL
                     RRGPG"
     gene            complement(2013..2312)
                     /locus_tag="VF958_11165"
     CDS             complement(2013..2312)
                     /locus_tag="VF958_11165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012545961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HEX9149710.1"
                     /translation="MNVGGAFLGAALEVFFAILSVIQWLVIIAAVISWVNPDPRNSIV
                     QFLYRTTEPILRPFRTILPPRRTGGIDFSPILVILLILFLKVFLRTLFMNAARAF"
     gene            complement(2309..3025)
                     /locus_tag="VF958_11170"
     CDS             complement(2309..3025)
                     /locus_tag="VF958_11170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020741349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HEX9149711.1"
                     /translation="MTEPLSEAELAGRREEVLSRIADAAGRAGRNPKEIALLAVSKGH
                     PAALVMNAAKAGFTLFGENRVAEGVRKIEAVRTRFPSLVWRLIGPLQTNKAKAALQFF
                     SAIESLDRERLAARMEGFLAAEGRRMPVLLEINVGGEATKSGASPQAVEGLARAALAC
                     PHLEVRGLMAVPPFDPDPEQARPYFRRLREIRDRLAERLGVALPELSMGMSHDFEVAV
                     EEGATEVRVGTALFGPRGAA"
     gene            complement(3022..3438)
                     /locus_tag="VF958_11175"
     CDS             complement(3022..3438)
                     /locus_tag="VF958_11175"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012984151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI family protein"
                     /protein_id="HEX9149712.1"
                     /translation="MKFRAFIDGAARGNPGPAGAGVFVEPEHGRPALEFYEPLGSTTN
                     NVAEYRALLLALERAEYAGADEVEIRSDSRLLVEQLRGNFKIRAEHLKPLLAESIIRA
                     KRFRSFSISHIPREQNSKADRLANKGADASERSTRF"
     gene            complement(3548..4261)
                     /locus_tag="VF958_11180"
     CDS             complement(3548..4261)
                     /locus_tag="VF958_11180"
                     /inference="COORDINATES: protein motif:HMM:NF014630.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-type zinc ribbon domain-containing protein"
                     /protein_id="HEX9149713.1"
                     /translation="MNTPLDQLYRLQDRIRFLHARERDRDTIPAELVEVDREYRVKVE
                     AADRLKKRRAEAEVERRRAHAELSEHREKLRKYQTQLRAVQNSREYGAVLNEIDGVEK
                     LVRSAEDRILALDSEIESSTKELASREELLPLETEQHEERLKDWRAAQRAINEELASA
                     QEEIGKLEASMHPRDRSEFQRLLDKKGGLAIVLVAGNSCSACHVKVRPAALQSLKAGR
                     EIVYCDSCKRILYWDMQRS"
     gene            complement(4413..4489)
                     /locus_tag="VF958_11185"
     tRNA            complement(4413..4489)
                     /locus_tag="VF958_11185"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4453..4455),aa:Ile,seq:cat)
     gene            complement(4525..6198)
                     /gene="rpoD"
                     /locus_tag="VF958_11190"
     CDS             complement(4525..6198)
                     /gene="rpoD"
                     /locus_tag="VF958_11190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011525093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HEX9149714.1"
                     /translation="MNLVVEEKYPEVQILYKEGKNKGYLLYEEIQTVISDELVGSPRE
                     LEQVYERFFELGIEIVNDPDRAARKRAALDAETAEEAREAVEKTNDPVRMYLREMGTV
                     KLLDREGEVRIAKRIEQGEAKIYQALASNPIVLEEILKLLENVGRDSRMVNELVNIPE
                     DAEDLDDRASQRIESVLGSFKKIGGLDKDIKRMKFKARTIKKGTKALARIEASIDKRI
                     ADMAKLIRAADFTPAVLNRAIGILKDIDRQMSVPESTIRQDTNTLKKEKNATRIDFYK
                     RRIAKYRKTLRELEQKFGVSHEDVKKTLRMVREGEEEADQAKHELIVANLRLVVSIAK
                     KYTNRGLQFLDLIQEGNIGLMKAVEKFEHRRGYKFSTYATWWIRQAITRAIADQARTI
                     RIPVHMIETINKLTRTSRSLVQELGREPNAEEIAKRMDMPVSKVRKIMKIAQEPISLE
                     TPIGEEEDSHLGDFIEDRQVLSPIDSVLVSNLQDQTRRVLKTLTPREEQVLKMRFGVG
                     DGSEHTLEEVGRSFNVTRERIRQIESKALRKLRHPSRAKKLKPFLETIR"
     gene            complement(6216..>7415)
                     /locus_tag="VF958_11195"
     CDS             complement(6216..>7415)
                     /locus_tag="VF958_11195"
                     /inference="COORDINATES: protein motif:HMM:NF024755.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HEX9149715.1"
                     /translation="PVGFSGRALSPEDEPKYLNSPESPIFLKKRLLYGLTEARESIRR
                     RERTVLAEGCFDHLALYLSGVEEAVASMGTALTPEQAAKLKRLAPRVVVCYDGDSAGR
                     NAARAALSHLLAQGLEVSVARLPADEDPDDVRRREGPEALARRIDEAPGYLPWLLEEL
                     RPAEPRITAEEKKDRIRAILETLSAIPDRILRYEEYRRVSQEVGVPVEVLWSSDSANR
                     SSATPSGSVGDPRRSAPAAPPPARQGPGTQGNGSVLSGGEIPAAERRLLQLLIQGGEH
                     ISLIRESLNAEHLTHPGVRGLVETFGKVGGTRGAVDFRSQLAHLKESSDISLVSRLAL
                     EESPEPTQEEILRLLRRLERRHLEREQATVQEAIRRAVAAEQGDELSRLLKRKQEIGK
                     RIVELGR"
BASE COUNT         1135 a         2491 c         2397 g         1392 t
ORIGIN      
        1 tcggcgagcg aagctcccat ggcgccggcg agggcggcgg cggtgccgcc tcccggcgtc
       61 ggcgcgtcgg aagcgagcgc cttgacgagg tcttcgaaag agagcttctc gaagttcacg
      121 actcggaact gacgatgagt ccgcctcgaa ccacggtcct gaccaggttg acgccccaat
      181 ggtagacgag gtggcggtcg tcgggggcgt cgagcacgac gaggtcggcg gttttgccgg
      241 gttcgagcgt cccgatgcgg tccgccaggc cgagcgacgc cgcggcgttc aaggtcagcc
      301 cggtcagcgt ttcctcgacg gtcaagcccg aatcgaggca ggcgtgcgcg gcgacggacg
      361 cgagggactc ggtgaagcag gtgcccgggt tcgcgtcgga ggccacggcg acgggtaccc
      421 ccgctgcgat catcgcccgc gcgggcgcgc gccgcgagcg catccaccat gcggtgccgg
      481 gcaggatcac ggcgacgata ccggaccgag cgagcgcggc gatctcgtcg tctccggcga
      541 aaaggaggtg gtccgcggaa gcggcgccga gctctgccgc gaggagcgtc ccgcccgatc
      601 gcgcgagctc gtcggcgtgg acgcgcaatc cgagcccgac ccggcgcgcc gcctcgagga
      661 tcgtccgcga ctccgcgacg gtgaagaccc cctcttcgca gaagacgtcg cagaagcggg
      721 cgagaccctc gcgcgcggcg cgcggaacga tctcgtcggc gacgatccgc acccactcct
      781 gccgccgctc gcggaattcc ggaggcactt cgtgcgcggc gagaagcgtc ggaacgatcg
      841 cgggaagatc cggcttttcg gcgagcgcct tcaggagacg gagcgagcgg agctcgtctt
      901 cgaccgtcag cccgtagccg gacttcgcct cgacggtcgt cgtgccgtgc gcgagcatcg
      961 cggcgagacg gcgccgcacg gtcgccgaga gctcgtcgtc ggaagccgcg cgagtcgccc
     1021 ggacggtcgc cagaattcct ccgccggcag cagctatatt cgcgtagctc tcgccggcca
     1081 gacgccgccc gatctcctct ccgcggtcgc cctcccagac cgggtgcgtg tgggcgtcga
     1141 cgaatccggg caggagcgtt ccgcccgccg catcgaccgt gactccggcg gggcgtcccg
     1201 agtactcgcg ccggtagtcg gcctcggtgc cgacgaactc cagaacgccg ttctccatcc
     1261 ggacgacggg atgccggatg cggcggattt ctcgctgttc gcggccgcgc cgggcgctcc
     1321 tgccgagcgg cgtcgcgagg caggcggccc cgcgaatctc gagggaggtc atgactgagg
     1381 actgaggact atggacggcg gactgatggg gctgtcaccc cgggattgat acccgggcaa
     1441 ttcctcgaca gtcctcagtc ctcagtcctc cggcctctgt cctccgtcgg ctagccggga
     1501 ccgcgtctca ggtaggacag cggacgatcc tgctcgtcct gcttgctgtc catctccagc
     1561 acctgctgga agagctccaa catcttctgg atctcgagac gaaagttcgc gcgcccgagc
     1621 ttgagctcgt gaatcgcctt ctcgacctcg gcggcgcgca gcatcgcgtt gtgcgtcaac
     1681 cgctccgcgg agtcctcggc ctcctggacg acgagctccg ccttggtctg agcagcggcg
     1741 cggatgtctt cggaggcacg ctgcgccgag aggagcgtgt ccttcaggat ccgttcgcgc
     1801 tcacggtgct ctgcgttctc ctcctcgagg ccgcggatac ggccgccgag cgccgcgttt
     1861 tcacgcgcga gtgtctccat ttcttccgcg gccatgtcga ggaacgcccc gacctcggtc
     1921 gggtcgaggc ccctccactt acgcgaaaaa cgctgcttct gaatctcgag gggcgtcagt
     1981 ctcgacatgt cgttcctccg gaagttgatc gttcagaaag cccgggcggc gttcatgaac
     2041 agggtgcgca gaaagacctt gagaaaaagg atgagcagga tcacgaggat cggcgagaaa
     2101 tcaattcctc ccgtgcgccg cggcggcagg atcgttcgga acggtcgaag gatcggctcc
     2161 gtcgtgcggt agaggaactg cacgatcgaa ttccgaggat cggggttcac ccaggagatc
     2221 acggccgcga tgatgacgag ccactggatc accgacagga tcgcgaagaa gacctccagg
     2281 gcggcaccca gaaacgctcc ccccacgttc atgccgcgcc cctcggtccg aagagcgccg
     2341 tgccgacgcg cacttcggtc gcgccctcct cgacggcgac ctcgaagtcg tggctcatcc
     2401 ccatcgagag ctcgggcagc gcgaccccga ggcgctcggc gaggcggtcc cggatttccc
     2461 ggaggcggcg gaagtagggt cgcgcctgtt ccgggtcggg atcgaacggc ggcacggcca
     2521 tgagtccccg aacctccagg tgcggacagg cgagcgcggc acgcgcgagc ccttcgaccg
     2581 cctgcgggga ggcgcccgac ttcgtcgctt cgccgccgac gttgatctcg agcaaaaccg
     2641 gcatccgtct tccttccgcg gcgaggaatc cctccatccg cgcggcaagc ctttctcgat
     2701 cgaggctttc gattgctgaa aagaattgta gcgccgcctt cgccttgttg gtctggagag
     2761 gcccgatcag ccgccagacg agcgagggaa accgcgtgcg gacggcctcg atcttgcgaa
     2821 cgccttcggc gacccggttc tccccgaaaa gggtgaaacc ggccttcgcc gcgttcatga
     2881 cgagcgcggc cgggtgcccc ttgctcaccg cgaggagagc gatttctttc gggttgcggc
     2941 cggcgcgccc ggccgcgtcg gcaatacgcg agagaacttc ctcgcgacgg cccgcgagct
     3001 cggcttcgga aagaggctcg gtcaaaaccg cgtcgatctc tcgctcgcgt ccgcgccctt
     3061 gttcgcgagc cgatccgcct tcgagttctg ctcgcgcggg atgtggctga tcgaaaagct
     3121 gcggaatcgt ttcgcgcgga tgatcgactc ggcaaggagc ggcttcaggt gttccgcccg
     3181 gatcttgaag ttgccgcgca gctgctcgac gaggagccgg ctgtcggagc ggatctcgac
     3241 ctcgtccgcg ccggcgtatt ccgctcgctc gagcgcaagg agcagggcgc ggtattccgc
     3301 gacgttgttc gtcgtcgaac cgagcggctc gtagaactcg agcgcgggcc gaccgtgttc
     3361 cggctcgacg aagacccccg ctcccgccgg acccgggttc ccgcgcgcgg ctccgtcgat
     3421 gaaggcgcgg aacttcacgg ggtcagcatc acggggtcag gtctccactt gccacattgc
     3481 caccgaattg tggcaagacc agctcggcag aatcactggc gccgcattct gtgaagacct
     3541 gaccccgcta cgaccgctgc atgtcccagt acaggatccg cttgcagctg tcgcagtaga
     3601 cgatctctcg tccggctttc agggactgga gcgccgccgg tcgcactttc acgtggcagg
     3661 ccgagcacga gttgccggcg acgagcacga tggcgaggcc gcccttcttg tcgagaaggc
     3721 gctggaactc ggagcggtcg cgaggatgca tcgacgcctc gagcttgccg atctcctcct
     3781 gcgccgatgc gagctcctcg ttgatcgccc gctgcgctgc acgccagtcc ttcaggcgct
     3841 cctcgtgctg ctcggtctcg agcggcagca gctcttcccg gctcgcgagc tccttcgtcg
     3901 agctctcgat ttcggagtcg agggcgagga tccggtcctc cgcgcttcgg acgagcttct
     3961 cgacgccgtc gatctcgttc aggaccgcgc cgtattcccg gctattctgg accgctcgga
     4021 gctgcgtctg gtacttgcgg agcttctcgc ggtgctcgga gagctccgcg tgcgcgcgcc
     4081 gccgttcgac ctcggcctcg gcgcggcgct tcttcagccg atcggccgcc tcgaccttga
     4141 cccggtactc ccggtcgacc tcgaccaatt ccgcggggat cgtgtcgcga tcccgttccc
     4201 gcgcatgcaa gaagcggatg cggtcctgga gccggtagag ctgatcgagc ggggtgttca
     4261 cgccggacct cccgctcgtc tcacgcgacc acttcgagcg gagatgaaaa agggtgttcc
     4321 cgcgtggaac accctttcag gaagaacttt ttcggctctc gtcggtctcg gttcgaactc
     4381 ctcaatctcc ctcgtcctct ataacggcgc tctggtgggt ccacccggat tcgaaccggg
     4441 gtctgaccgg ttatgagccg gtggctctgg accgctgagc tatggacccc ggcgctcgat
     4501 tgcgcccgcc aaatatatag cgagttaacg gatcgtttcc aggaacggct tcaacttctt
     4561 cgcccgcgac ggatggcgga gcttgcgcaa cgccttggac tcgatctgcc ggatccgctc
     4621 gcgagtgacg ttgaacgacc ggccgacctc ttcgagcgtg tgctcggagc cgtcgccgac
     4681 gccgaaccgc atcttcagga cctgctcctc ccgcggcgtg agcgtcttca atacccggcg
     4741 ggtctggtcc tgcaggttcg agacgagaac cgagtcgatc ggcgagagga cctgccggtc
     4801 ctcgatgaaa tcgcccagat gcgaatcctc ttcctctccg atcggcgtct cgagcgagat
     4861 cggctcctgc gcgatcttca tgatcttgcg gaccttcgag accggcatgt ccatgcgctt
     4921 ggcgatctcc tcggcgttcg gctctcggcc gagctcctgg acgagcgagc gggacgtgcg
     4981 cgtcagcttg ttgatcgttt cgatcatgtg gaccgggatg cggatcgtcc gcgcctggtc
     5041 cgcgatcgcg cgcgtgatcg cctggcggat ccaccacgtg gcgtaggtcg agaacttgta
     5101 tccccgccgg tgctcgaact tctcgacggc cttcatgagg ccgatgtttc cttcctggat
     5161 caggtcgagg aactgcagcc cgcggttcgt gtacttcttc gcgatcgaca cgacgaggcg
     5221 gaggttcgcg acgatgagct cgtgcttggc ctgatcggcc tcctcttccc cttcgcgcac
     5281 catgcgcagc gtcttcttga cgtcttcgtg cgagaccccg aacttctgct cgagctcgcg
     5341 aagagtcttg cggtacttcg cgatccgccg cttgtagaag tcgatgcggg tggcgttctt
     5401 ctctttcttg agagtgttgg tgtcctggcg gatcgtcgac tcgggaaccg acatctgccg
     5461 atcgatgtcc ttcaggatgc cgatcgcgcg gttgagcacg gcgggagtga agtcggcggc
     5521 gcggatcaac ttcgccatgt ccgcgatccg cttgtcgatc gacgcttcga tgcgcgcgag
     5581 ggccttcgtc cccttcttga tcgtgcgcgc cttgaacttc atccgcttga tgtccttgtc
     5641 gaggccgccg atcttcttga agcttccgag caccgactcg atccgctgcg aggcccggtc
     5701 gtcgaggtcc tcggcatcct ccgggatgtt gacgagctcg ttgaccatgc gcgagtcgcg
     5761 tcccacgttt tcgaggagct tcaggatctc ttcgaggacg atcgggttgc tcgcgagcgc
     5821 ctggtagatc ttcgcctcgc cctgctcgat gcgcttggcg atgcggactt ccccctcgcg
     5881 gtcgaggagc ttgaccgtgc ccatctcgcg caggtacatg cgcaccgggt cgttcgtctt
     5941 ctcgacggct tcgcgggctt cctcggcagt ctcggcgtcg agcgccgcgc gcttcctcgc
     6001 ggcccgatcg ggatcattga cgatctcgat cccgagctcg aagaatcgct cgtaaacctg
     6061 ctcgagctcg cgggggctcc cgacgagctc gtcggagatc accgtctgga tctcttcgta
     6121 gagcagatag cccttgttct tcccctcctt gtagaggatc tggacctccg gatacttttc
     6181 ctcgactacc aaattcattc ctttcgccgg ggctttcaac gcccgagctc cacgattcgc
     6241 ttcccgatct cctgttttct cttgagcagc cgcgacagtt cgtctccctg ctccgcggct
     6301 accgcccgac ggatggcttc ctgcaccgtc gcctgttccc tttccagatg tctccgctcg
     6361 agccgtcgga gcagcctcag aatctcctct tgcgtgggct cgggactctc ctcgagcgcg
     6421 agtcgggata ccagagaaat gtcgctcgat tccttaaggt gagccagctg acttcgaaaa
     6481 tcaaccgctc cccgcgtgcc cccgactttt ccgaacgttt cgaccagccc ccggacaccc
     6541 ggatgagtca ggtgttccgc gtttaaagac tctcgaatca aagagatgtg ctccccgccc
     6601 tgaatcagca gctgcaggag ccggcgttcc gcggcgggta tctccccacc cgataacacc
     6661 gatccatttc cttgcgttcc cgggccctgc cgggcgggag ggggtgctgc gggagcgctc
     6721 cgccgggggt ccccgacgga gccggatggg gttgcagacg accggttcgc ggaatcgctc
     6781 gaccagagga cttctacggg gacaccaacc tcctgtgaga cgcgtcggta ctcctcgtac
     6841 cggaggatcc ggtcgggaat cgcagagagg gtttccagga tcgcgcggat cctgtctttc
     6901 ttttcctccg cggtaatccg aggctccgca ggccggagct cctcgagcag ccacggaagg
     6961 tatcccggcg cctcgtcgat ccttcgcgcg agggcttccg ggccttcccg gcggcggacg
     7021 tcgtcggggt cctcgtcggc gggaaggcgg gcgacggaga cttcgagccc ctgcgccaac
     7081 aggtgcgaga gtgccgcccg cgcggcattc cggcccgcgg agtcgccgtc gtagcagacg
     7141 acgactcgcg gcgcgagccg cttcagcttc gccgcctgct caggggtcag cgccgtgccc
     7201 atcgaggcga ccgcctcctc cactcctgag agatagagcg caaggtggtc gaagcaacct
     7261 tcagccagga cggtcctctc acgccggcga atcgactcgc gtgcttcggt gaggccgtag
     7321 aggagcctct tcttcaagaa gatcggcgac tccggagagt tcagatattt tggctcgtcc
     7381 tcgggagaga gcgcgcggcc actgaagccg acggg
//
LOCUS       DASWAV010000535         4037 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U2665
            k141_190993, whole genome shotgun sequence.
ACCESSION   DASWAV010000535 DASWAV010000000
VERSION     DASWAV010000535.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960316
            Sequence Read Archive: SRR10260007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 4037)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4037)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:47:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,929
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4037
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_190993"
                     /isolate="SMAG_U2665"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.36444 N 132.31889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..219
                     /locus_tag="VF958_11200"
     CDS             <1..219
                     /locus_tag="VF958_11200"
                     /inference="COORDINATES: protein motif:HMM:NF013044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0044237 - cellular metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HEX9149716.1"
                     /translation="EWFFPDPGRTSVQETVRQTIEFSDEIQREDIAICEAVQKGLESR
                     TYDLGRFSVARESGVHHFHRLYASALSP"
     gene            300..1778
                     /locus_tag="VF958_11205"
     CDS             300..1778
                     /locus_tag="VF958_11205"
                     /inference="COORDINATES: protein motif:HMM:NF013594.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HEX9149717.1"
                     /translation="MVRIHRFERVAAAASVAFAIASGTATAQTQVKAGFNVFSAEQDV
                     EIGRQSAVEAERQLPILRSSSAESYVDRVGRRLAASTPGPKFPYSFKVVNVSDINAFA
                     LPGGPVYVTRGVLETVRNEGELAGVIAHEISHVALRHGTNQASKAYLAQAGIGILGGV
                     LGGGGSATGQIVQAVGGFGLNAMFLKFSRTAESQADILGTQIMARAGYDPNAMATMFE
                     LLKGQQGREPGRLAQFFSDHPSPANREVRVRQEAQAVGGRGTTSVVGGLEGVRADLSR
                     MPRAGTMSQIAPGATSSGSAPAGAIERPSTRMRTFRQRSGFFEIQYPENWRVAESSGY
                     GVTILPEGGAVRTPSGQDALIYGVVLNHYVPFEGSGGDVWNQPRGPVSGQGSLEQATN
                     DLVNQLLQGNTHLRLVSNSMRRQTIDNARALSVLLAGTSPVTRQEERVTVFTRELPDG
                     HVIYALFVAPGRDYSALSDTFQKIISSLKVNDKSYHQENAAR"
     gene            complement(1862..2383)
                     /locus_tag="VF958_11210"
     CDS             complement(1862..2383)
                     /locus_tag="VF958_11210"
                     /EC_number="5.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012521.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate mutase family protein"
                     /protein_id="HEX9149718.1"
                     /translation="MPRLPVGKRLVVTLLLFAAAASAAAQRAIILVRHAEKAVDANEA
                     GVPLSEAGHARAARLARMLAGAGITEIYATETDRARQTAEPLAKALGLAVRTYSPRDA
                     AGKLAPRILIDRLKKDDAGVVLVVGHQNTVPDVIGALGYRGKVEIGDGDYDDLFVVVP
                     KTGAAPAVIRLKY"
     gene            2441..>4037
                     /locus_tag="VF958_11215"
     CDS             2441..>4037
                     /locus_tag="VF958_11215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011626132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008241 - peptidyl-dipeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M2 family metallopeptidase"
                     /protein_id="HEX9149719.1"
                     /translation="MIETFRCCRVALPFIVVLFVAASSPAAVVKKAAPARGKAPTVAE
                     AKAFAEEAETKLLALSVEASRAGWVQSTYITDDTEALAAKANERAINAGVEYAKKATR
                     FDGLKLPADTARKLMILKNGLTLAAPADPKESEEVTRIAAAMEGEYGKGKYCPQGKDK
                     CLDLQDITKIMAESRDGGELLDVWRGWHTISIPMRPEYRRFVELANKGARELGFKDSG
                     AMWRSKYDMPPDAFAKEVDRLWEQLKPLYVSLHAYVRWKLREKYGDVVPANGPIPAHL
                     LGNMWAQDWSNVYDLVAPKDADPGYDLTKILKAKNLDAKEMVRYGERFFTSLGFAPLP
                     DTFWERSLFVKPRDREVVCHASAWDVDNVNDLRIKMCIDTTAEDFTTIHHELGHNFYQ
                     RAYNTKPVLFRDSANDGFHEAVGDTIALSITPEYLVKIGLLDKAPDPSKDIGLLLNRA
                     LEKLAFLPFGLIVDQWRWKVFDGSITPANYNATWWDLRTRYQGVAPSVARSENDFDPG
                     AKYHVAGNVPYTRYFLAAILQFQF"
BASE COUNT          710 a         1344 c         1355 g          628 t
ORIGIN      
        1 gagtggttct tcccggaccc cgggcgaacc tcggtgcagg aaaccgtccg gcagacgatc
       61 gagttctccg acgaaatcca gcgcgaagac attgcgatct gcgaggcggt ccagaagggc
      121 ctcgagtcga ggacatacga cctcgggcgc ttctcggtcg cgcgcgagag cggcgtgcac
      181 cacttccatc ggctctacgc gtcagccctc agtccatagc tcccggtccg cagaccctgg
      241 cactcccctt gcgtttcccc cagcgcacgt ccgtgcgcga gcgcggagag gaggggttca
      301 tggtgcgaat tcacaggttc gagcgggtgg cggcagccgc cagcgtcgca ttcgcgatcg
      361 cgtccgggac cgcgacggcc cagacccagg tcaaggccgg cttcaacgtc ttctcggcgg
      421 agcaggacgt cgagatcggc aggcagtcgg ccgtggaagc ggagcgccag ctcccgatcc
      481 tccggagctc gtcggccgag tcctacgtcg accgcgtcgg ccgccgcctc gcggcctcga
      541 cgcccggtcc gaaatttccg tactcgttca aggtcgtgaa cgtgtccgac atcaacgcct
      601 tcgcgctgcc gggaggtccc gtctacgtga cgcgcggcgt cctcgagacg gtccgcaacg
      661 agggggaact cgccggggtg atcgcgcacg agatctcgca cgtggccctg cggcacggca
      721 cgaaccaggc gtccaaggcc tatctcgcgc aggctggcat cgggatcctc gggggcgttc
      781 tcgggggcgg cggaagcgcg accgggcaga tcgtccaggc ggtcggcggg ttcgggttga
      841 acgcgatgtt cctgaagttc agccggaccg cggagtccca ggcggacatc ctcggaacgc
      901 agatcatggc ccgcgccgga tacgacccga acgcgatggc cacgatgttc gagcttctca
      961 aggggcagca gggacgcgag cccggcaggc tcgcgcagtt cttcagcgat cacccgtcac
     1021 cggcgaaccg ggaggttcgc gtccggcagg aggcgcaggc ggtcggaggg cgcggaacca
     1081 cgtccgtcgt cggagggctc gagggcgtga gagcggacct ctcgcgcatg ccgcgggcgg
     1141 gaaccatgtc ccagatcgcg cccggcgcga cttcgtcggg ctcggctccc gccggcgcga
     1201 tcgagcgccc gtcgacgagg atgcggacct tccgccagcg cagcggattc ttcgagattc
     1261 agtatcccga gaactggcgc gtggccgagt cgagcggcta cggcgtcacg atcctgcccg
     1321 agggcggagc tgtccggacc ccgagcggcc aggacgcgct catttacggc gtggtcctga
     1381 accactacgt tcccttcgag ggatcaggcg gcgatgtgtg gaatcagccg cgcggcccgg
     1441 tatccgggca ggggtcgctc gagcaggcga ccaacgacct cgtcaaccag ctcctgcagg
     1501 ggaacacgca ccttcgcctg gtctccaact ccatgcgccg ccagacgatc gacaacgcgc
     1561 gcgcgctgtc ggtgctgctc gccgggacgt cgccggtgac ccgccaggag gagcgcgtga
     1621 ccgtgttcac gcgcgagctg ccggacggcc acgtgatcta cgcgctcttc gtcgcgcccg
     1681 gacgcgacta ctcggctctc tccgacacgt tccagaaaat catctcgagc ctcaaagtga
     1741 acgacaagtc gtaccaccag gagaacgccg cgaggtgaga tcgcggcact gcgcgaggcg
     1801 cccccgcagg aactccctct ccctccggga gagggggggt gagggggctg attgagctct
     1861 ctcagtactt cagccggatg acggcgggag cggccccggt cttcggaacg acgacgaaga
     1921 ggtcgtcgta atcgccgtcg ccgatctcaa ccttgccccg gtacccgagc gccccgatga
     1981 catcggggac ggtgttctgg tgtccgacga cgagcacgac cccggcgtcg tccttcttca
     2041 ggcggtcgat gaggatccgg ggcgcgagct tgcccgccgc gtcccgagga gagtacgtcc
     2101 gcacggcgag cccgagcgct ttcgcgagag gctcggccgt ctgccgcgcg cggtccgtct
     2161 cggtggcata aatctccgtg atgccggcgc ccgcgagcat ccgtgcgagg cgcgcggctc
     2221 gcgcatgccc tgcttccgac agcggcaccc ctgcctcgtt tgcgtcgacg gccttctcgg
     2281 cgtggcgcac gaggatgatc gcccgctgag cggccgcgga ggcggcggct gcgaacagaa
     2341 gcagtgtgac aacaagtcgc ttcccgacgg gcagccgagg cacgaggtcc aaggtagcac
     2401 gaactctgcg gtagatttgg ccgcccgatg ggaggaccga atgatcgaaa cgtttcgttg
     2461 ttgccgggtc gcgcttccct tcatcgtcgt cttgttcgtg gccgcctctt ctccggccgc
     2521 agtggttaag aaagccgctc cggcgcgggg caaggctccc accgttgcag aggcgaaggc
     2581 cttcgccgaa gaagccgaga cgaagcttct cgcgctctcg gtcgaggcga gccgcgccgg
     2641 gtgggtgcag tccacgtaca tcaccgacga caccgaagcc ctcgccgcga aggcgaacga
     2701 acgggcgatc aacgccggcg tcgagtacgc caagaaagcg acccgattcg acggtctgaa
     2761 gcttccggcc gacactgcgc gcaagctcat gatcctgaag aacggcctga ccctggcggc
     2821 tccggccgat ccgaaggaga gcgaagaggt cacgcgcatc gccgcggcga tggaagggga
     2881 gtacgggaaa ggcaagtact gccctcaagg caaggacaag tgcctcgacc tccaggacat
     2941 cacgaaaatc atggcggaga gccgcgatgg gggagagctc ctcgacgttt ggcgcggctg
     3001 gcacacgatc tcgattccga tgcggccgga gtaccggcgc ttcgtcgagc tcgccaacaa
     3061 aggggcgcgg gagctcgggt tcaaggactc gggcgcgatg tggcggtcga agtacgacat
     3121 gcctccggac gccttcgcga aagaggtcga ccggctctgg gagcagctga agccgctcta
     3181 cgtttctctt cacgcctacg tgcgctggaa gctccgggag aagtacggcg acgtcgtccc
     3241 cgcgaacgga ccgatcccgg cgcacttgct cggcaacatg tgggcccagg actggtcgaa
     3301 cgtctacgac ctcgtcgcgc cgaaggacgc cgatcccggc tatgacctca cgaagatcct
     3361 caaggcgaag aacctcgacg cgaaggaaat ggtccgctac ggcgagcgct tcttcacgtc
     3421 actcgggttc gcgccgcttc ccgacacgtt ctgggagcgg tcgctcttcg tcaagccgcg
     3481 cgaccgcgag gtcgtgtgcc acgcgagcgc ctgggacgtc gacaacgtca acgacctgcg
     3541 catcaagatg tgcatcgata cgaccgcgga agacttcacg accattcacc acgagctcgg
     3601 gcacaatttc taccagcgcg cgtacaacac gaagcccgtg ctcttccgcg acagcgccaa
     3661 cgacgggttc cacgaggcgg tcggcgacac gatcgcgctc tcgatcacgc cggagtacct
     3721 cgtcaagatc ggcctcctcg acaaggcccc ggacccttcg aaggatatcg ggctgctcct
     3781 gaacagggca ctcgagaagc tcgccttcct cccgttcggg ttgatcgtgg atcagtggcg
     3841 atggaaggta ttcgacgggt cgatcacgcc ggcgaactac aacgcgacct ggtgggatct
     3901 gcggactcgg taccaggggg tcgcgccctc agtcgcgcga tccgaaaacg acttcgatcc
     3961 gggcgcgaag tatcacgtcg cggggaacgt gccctacacg cggtacttcc tcgcggcgat
     4021 cctgcagttc cagttcc
//
LOCUS       DASWAV010000536         4160 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U2665
            k141_510703, whole genome shotgun sequence.
ACCESSION   DASWAV010000536 DASWAV010000000
VERSION     DASWAV010000536.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960316
            Sequence Read Archive: SRR10260007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 4160)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4160)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:47:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,929
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4160
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_510703"
                     /isolate="SMAG_U2665"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.36444 N 132.31889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2097)
                     /locus_tag="VF958_11220"
     CDS             complement(<1..2097)
                     /locus_tag="VF958_11220"
                     /inference="COORDINATES: protein motif:HMM:NF018899.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="POTRA domain-containing protein"
                     /protein_id="HEX9149720.1"
                     /translation="MIRLGTPDRLSAQQSTPQPRPLVGETVVSLAYTTDGPVDRSEVA
                     RLVSIKAGRPLTEEVTGSTIRHLFATRRFADVRIEAEPAEGGVAVTVHLFRSFRVNPL
                     RFDDGVSVPKEEMRRTIPFAEGAVFQAEQLEVGSAALKRRLDAEGYISSTVRPEVFFD
                     RTTFDAEVIYHIVAGKPARTAPPLFDGDIQPFTREELLKKARLDPGDRYRESRARADA
                     DRISDYLKKKMRLRGTVELIAAQPTDDGRVMPVYRIAIGPRVVFQTRGIREKKVRREV
                     TDLLEGQLFDEDLILQYAENKRRALQRKGYYRAKVEYTISPATDVQTVTINVDPGRKF
                     AVEKIAIDGNQAVSDRKLRPLLVTQEKGLPLVRSGRLVDDVLQADADAVLGYYQTHGW
                     VNAKVETPAITEGSKPDRLVVRFKIEEGPRALVTTRKVVGAEHVSDDQLEEILSVSVG
                     EPFNPNQVRQDSFSLQNHYRNRGWREAAVKDDWKLSSDGTSAEVTYTVEEGMRSFFGK
                     TIVRGNTRTHTNRIARLVTWDEGDPFSEQEVLTTQRNLTRTSVFRRVEVKPQPADPSN
                     QVRPVDIDVEEGRPLSLLYGVGYQYAPDALANRSDPFAIAGVSYNNLFGTMRSASIEV
                     QYAPISKRGRLIMTFREPFLFNRNVPLNVLFFLTREPIQKIDIERVGTVLESSKLFGR
                     YLRVGMRYEYQRIDAVN"
     gene            complement(2160..>4160)
                     /locus_tag="VF958_11225"
     CDS             complement(2160..>4160)
                     /locus_tag="VF958_11225"
                     /inference="COORDINATES: protein motif:HMM:NF016260.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translocation/assembly module TamB
                     domain-containing protein"
                     /protein_id="HEX9149721.1"
                     /translation="RFSPGRFELADVRAELGGGMVGGSGAIQIQEKTFEARFAGDAIP
                     LESITALSDASKDITGKLSFQVSGSGEIERPDLTATATLADATIFGHSIPDANEPRLD
                     VRLVRGELAGSVTVADRWSLTAAGNLFEPGSRVTVNLAAPDLAALLLFTPLELQPGRG
                     GALALRGELTLPATEKDFLSGVFTVTEARLDFPDRPGVLRTSGDVRISLARQKLVFDE
                     FHAVGEGTDLTVRGSVDVSPEHALAVTVAGPIDASLVELAVPGTDLNGRLTVQIAAGG
                     TFAAPDLSGTLRIENGRYRLTGLTQIVDDIDGVISLHGSHAEIDGIRARVGGGDLFAA
                     GSINLKGIGVSDFRLTIQARHVAVRYPEDMRLVVDADLVATGAPGSGQVVRGEVTLLR
                     GTYSRDFDVTLASLVERGRPSGAVAAREPWKEETALEVRIVSAAALEVRNNVARLTAE
                     VDLVARGTVAQPILLGQVLFDEGGRITFRDVQYEIESGTITFANTARIAPILDLRARA
                     EVKGYDLVVNLVGTWPRIQSTFTSDPPLSDEAVIGLLLTGAEPNQRTGSDTTQSLVSA
                     AGGILAGALAGGVTRPTQRLFRLDRFQIDPVFTGSQLTDIRSTVGKQITPDLLVTYSQ
                     SLNSDRESVVQLEWRVTSSITLQALRDENGVYSINVRRRQRL"
BASE COUNT          638 a         1419 c         1416 g          687 t
ORIGIN      
        1 gttgaccgcg tcgatccgct ggtactcgta gcgcatcccg acccgcagat agcggccgaa
       61 gagcttcgaa gactcgagca cagtgccgac ccgctcgatg tcgatcttct ggatcggctc
      121 gcgggtgagg aaaaaaagca cgttcagcgg cacgttgcgg ttgaagagga atggctcgcg
      181 gaaagtcatg atcaaccggc cgcgtttcga gatcggcgcg tactggacct cgatcgaggc
      241 cgaacgcatg gtcccgaaca ggttgttgta cgaaacgccg gcgatcgcga aggggtcgct
      301 ccggttcgcc agcgcgtccg gcgcgtactg atatccgacg ccgtacagca gcgacagcgg
      361 cctcccctcc tccacgtcga tgtcgacggg ccgcacctgg ttcgacgggt cggcgggctg
      421 cggcttgacc tccacgcgcc ggaagacgct ggtgcgcgtc aggttcctct gcgtcgtgag
      481 cacttcctgc tcggaaaacg ggtcgccttc gtcccaggtg acgagccgcg cgatccgatt
      541 ggtgtgggtc cgcgtgttgc cgcgcacgat cgtcttcccg aagaacgagc gcatcccctc
      601 ttcgacggtg tacgtcacct cggccgaggt tccgtccgac gagagcttcc agtcgtcctt
      661 caccgcggcc tcgcgccagc cgcggttgcg gtaatggttc tggagcgaaa acgagtcctg
      721 ccgcacctgg ttcgggttga aaggctcgcc cacgctcacg ctcaggatct cctcgagctg
      781 gtcgtccgac acgtgctcgg cgccgaccac ctttcgcgtc gtgacgaggg cgcgcggccc
      841 ctcttcgatc ttgaagcgca cgacgagccg gtcgggcttc gagccctcgg tgatcgcggg
      901 ggtctcgacc ttcgcgttca cccacccgtg cgtctggtag tagccgagga ccgcgtcggc
      961 atccgcctgg agcacgtcgt cgacgaggcg ccccgaccgc accagcggaa gtcccttctc
     1021 ctgcgtgacg aggagcggcc ggagcttgcg gtcggacacc gcctggtttc cgtcgatcgc
     1081 gatcttctcc acggcgaact tccgtcccgg atcgacgttg atcgtaacgg tctgaacgtc
     1141 ggtggccggg gatatcgtgt actcgacctt cgcgcggtag tagcccttgc gctggagcgc
     1201 gcgccgcttg ttctcggcgt attggagaat caggtcctcg tcgaagagct gtccctcgag
     1261 aaggtcggtc acttcccgcc ggaccttttt ctcgcggatt ccgcgtgtct ggaacaccac
     1321 ccgggggccg atggcgatgc ggtagaccgg catgacgcga ccgtcatcgg tcggctgcgc
     1381 ggcgatgagc tcgacagtgc ctcgcaaccg catcttcttc ttcaggtagt cgctgatccg
     1441 gtccgcgtcc gcgcgagccc tcgactcgcg gtaacgatcc cccgggtcga gccgggcctt
     1501 cttcagcaat tcctcgcggg tgaacggctg gatgtctccg tcgaagagcg gtggcgccgt
     1561 gcgcgcgggc ttgcccgcga cgatgtggta gatgacctcg gcgtcgaacg tcgtgcggtc
     1621 gaagaagacc tccggccgca ccgtggacga gatgtacccc tccgcgtcga ggcggcgctt
     1681 cagcgcggcg gagcccacct ccagctgctc ggcctggaag accgcccctt ccgcgaaggg
     1741 aatcgtccgg cgcatctcct ccttcggaac cgagacgccg tcgtcgaacc tgagcgggtt
     1801 cactcgaaag gagcgaaaga ggtggaccgt caccgcgacc ccaccttccg ccggctccgc
     1861 ctcgattcgg acgtcggcga atcggcgtgt ggcgaagagg tgccggatcg tcgagccggt
     1921 cacctcctcc gtgagcgggc gtcccgcttt gatcgagacg aggcgcgcga cctcgctcct
     1981 gtcgacgggg ccgtcggtgg tgtaggcgag ggaaaccacc gtctcgccga cgagcggccg
     2041 cggctggggc gtcgattgct gcgcggagag acgatcgggg gtcccaagac ggatcacggc
     2101 cgccagcagc gcgaagcccg ccgcccgcga ccggctgcgc gctcggaagc cgcctccgat
     2161 caaagtcgct gccgccggcg gacgttgatc gagtaaaccc cgttctcgtc gcggagcgcc
     2221 tgcagcgtga tcgagctcgt cacccgccac tcgagctgca cgaccgattc cctgtcggag
     2281 ttgagcgact gcgaatacgt cacgaggagg tccggcgtga tctgcttgcc gacggtcgag
     2341 cggatgtcgg tcagctggct tcccgtgaag acgggatcga tctggaaccg gtccagccgg
     2401 aagagccgct gggtgggccg cgtcacgccc ccggcgagcg caccggcgag gatcccgccc
     2461 gccgcggaca cgaggctctg cgtcgtgtcg gagccggtgc gctggttggg ctccgctccg
     2521 gtcagcagca gcccgatcac cgcctcgtcc gagagcggcg gatcggacgt gaacgtcgac
     2581 tggattcgcg gccaggtccc gacgaggttc acgacgaggt cgtacccctt cacctcggct
     2641 cgcgcgcgga gatccaggat gggtgcgatg cgcgccgtgt tcgcgaaagt gatcgtcccg
     2701 gactcgatct cgtactgcac gtcgcggaag gtgatccggc caccttcgtc gaacaggacc
     2761 tggccgagga gaatcggctg cgcgaccgtg ccccgcgcga ccaggtcgac ctcggccgtc
     2821 aaccgggcga cgttgtttcg cacttcgagc gcggcggcgg acacgatccg gacctcgagc
     2881 gccgtctcct ccttccacgg ctctctcgcg gcgaccgcgc cgctcggacg tccccgctcg
     2941 acaagtgacg cgagggtcac gtcgaagtcg cgcgagtacg tcccgcgcag gagcgtgact
     3001 tcgccacgga ccacctgccc gcttcccggg gctcccgtgg cgacgaggtc ggcgtcgacg
     3061 accagcctca tgtcttccgg gtatcggacg gcgacgtgcc gggcctgaat ggtcagccgg
     3121 aagtccgaca ctccgatccc cttcaggttg atgctgccgg cggcgaagag atcgcctcca
     3181 ccgacgcgcg cccggatgcc gtcgatctcg gcgtgcgagc cgtggagaga gatcacgccg
     3241 tcgatgtcgt cgacgatctg ggtcaggccg gtcaaccggt agcgcccgtt ttcgattcgc
     3301 agagtgccgg agaggtcggg cgcggcgaac gtgcccccgg cggcgatctg gacggtgaga
     3361 cgcccgttca gatcggtccc cggaaccgcc agttcgacga gcgacgcgtc gatcggaccg
     3421 gcgaccgtca ccgcgagcgc gtgttcgggt gagacgtcga ccgagccgcg gacggtcagg
     3481 tcggttcctt ccccgaccgc gtggaattcg tcgaacacga gcttctgccg cgcgagtgag
     3541 atgcggacgt cgccggacgt ccgcagcacc ccggggcggt ccggaaagtc gagccgcgct
     3601 tccgtgaccg tgaagacgcc ggagaggaag tccttctcgg tggcagggag cgtcagctcg
     3661 cctcgcaagg cgagcgcgcc cccgcgcccc ggctggagct cgagcggagt aaagagaagg
     3721 agcgccgcca ggtcgggcgc cgcgagattc accgtgacgc gggagccggg ctcaaacagg
     3781 ttcccggccg cggtcaggct ccaccggtct gccaccgtca ccgatccggc gagctcgccg
     3841 cgcacgagcc ggacgtcgag gcggggctcg ttcgcgtccg ggatggagtg gccgaagatc
     3901 gtcgcgtccg cgagagtcgc ggtggcggtc aggtcgggcc gctcgatctc gcccgaaccg
     3961 ctcacctgga acgacagctt tccggtgatg tctttcgacg cgtccgaaag cgccgtgatc
     4021 gactcgagcg gaatcgcatc gcccgcgaag cgcgcctcga acgtcttctc ctggatctgg
     4081 atcgcgccgc tcccgccgac cattccgccg ccgagctccg cgcggacgtc cgccagctcg
     4141 aaccggcccg gcgagaaccg
//
LOCUS       DASWAV010000537         1533 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U2665
            k141_191071, whole genome shotgun sequence.
ACCESSION   DASWAV010000537 DASWAV010000000
VERSION     DASWAV010000537.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960316
            Sequence Read Archive: SRR10260007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 1533)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1533)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:47:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,929
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1533
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_191071"
                     /isolate="SMAG_U2665"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.36444 N 132.31889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            130..699
                     /locus_tag="VF958_11230"
     CDS             130..699
                     /locus_tag="VF958_11230"
                     /inference="COORDINATES: protein motif:HMM:NF014038.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class IV adenylate cyclase"
                     /protein_id="HEX9149722.1"
                     /translation="MTDAARRLEVEIKVPARDLGAVRNKLRDEGARPLAPAHFESNDL
                     FDRGDGELANRGCALRLRRTDGGSTLTFKGLARFEAGIKTREERETAVSDPEEMERIL
                     AALGLERRFRYEKRREEWELEGCAIALDETPIGDFAEVEGEPPAIRRVLQRLGLDFSQ
                     AILDSYAELYQSRRRHEPSLPKDMVFRNR"
     gene            736..>1533
                     /locus_tag="VF958_11235"
     CDS             736..>1533
                     /locus_tag="VF958_11235"
                     /inference="COORDINATES: protein motif:HMM:NF024214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NDP-sugar synthase"
                     /protein_id="HEX9149723.1"
                     /translation="MLLAAGRGVRFRPVTETIPKPLFPYLNVPLVRAHLRRLADSGVR
                     YVGINLHHLGNSIEGHLNDPPPVDSRIRFFREPEILGTAGALANAAEWLRDDDFLVVN
                     SDAAIEPDYSSLVARHRESRRAATLLVTENREPARFTPLGAQRDRIVSFGVESAWPLL
                     YTGVCALSPRLLLRIAAGERSLVRDLWQPLLDEGREEIGWLLHEGPFSDLGTPHDFLR
                     ATRQALTRGGTFAKEAGRFDATSRVLTLERSKGFEAVSSVIGRSSIGR"
BASE COUNT          249 a          528 c          512 g          244 t
ORIGIN      
        1 cgcggagaaa ggtttctcct cggcgcgcga cgtcgggctc gtgatgaagg acctgatggc
       61 ggcgcaccgg ggacgggtcg acggaaagcg ggcgcaggaa atcgcccggc ggctgctagc
      121 gtgacggcaa tgaccgacgc cgcgcgccga ctcgaagtcg agatcaaagt cccggcgcgc
      181 gacctcggtg cggtccggaa caagctccgg gacgaaggag cgcggccgct cgcccccgcc
      241 cacttcgagt cgaacgacct tttcgatcgc ggcgacggag agctcgcgaa tcgtggctgc
      301 gccctgcgcc tgcgccggac ggacggcggc tcgaccctca ctttcaaggg gctggcccgc
      361 ttcgaagcgg ggatcaagac gcgcgaagag cgggagacgg ccgtgtcgga tccggaagag
      421 atggaacgga tcctcgccgc cctcggcctc gagcgacgct tccgatacga aaagcggcgc
      481 gaggagtggg agctcgaggg ttgcgccatc gccctcgacg aaacgccgat cggagacttc
      541 gcggaagtcg agggcgagcc tcccgcgatc cggcgcgtcc tccagcggct cgggctcgac
      601 ttctcccagg ccattctcga cagctacgcc gagctctacc aaagccggcg ccgccatgag
      661 ccgtccctgc cgaaagacat ggtgtttcgg aatcgctgag acgggggatc gctcggggag
      721 gccggtttga aggcgatgct ccttgccgcg ggccggggag tccggtttcg gccggtgacc
      781 gagacgatcc cgaaaccgct ctttccgtac ttgaacgtcc cgctcgttcg cgcgcacctc
      841 cgtcgtcttg cagactccgg agttcgatac gtcggaatca acctccacca cctcggcaac
      901 tccatcgagg gacatctgaa cgatccgccc ccggttgatt cccggatccg cttctttcga
      961 gagccggaga ttctcgggac tgcgggcgcg ctcgcgaatg ccgcggaatg gctccgcgac
     1021 gacgacttcc tggtcgtgaa ctcggacgcc gccatcgaac cggactactc ctcgctcgtc
     1081 gcccggcatc gggagtcccg ccgcgctgcg acgctcctcg tgacggagaa ccgggagccg
     1141 gcgaggttca cgccgctcgg cgcgcaacgc gaccgaatcg tttccttcgg agtcgagtcg
     1201 gcgtggccgc tcctctatac gggcgtgtgc gcgctctctc cgcgcctcct cctccggatc
     1261 gcggcgggag agcgctccct cgtgagggac ctgtggcagc ccctcctcga cgaaggacgc
     1321 gaagagatcg gctggctcct ccacgaaggg cctttctccg atctcggcac accccacgat
     1381 ttcctccggg cgacgcgaca ggctctgact cgcggcggca cgttcgcgaa agaggccggc
     1441 cggttcgacg ccacctcgcg ggttctcacg ctcgagcgtt ccaaaggatt cgaggccgtc
     1501 tcgagcgtga tcggacgctc gtcgatcggc cgc
//
LOCUS       DASWAV010000538         3072 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U2665
            k141_109719, whole genome shotgun sequence.
ACCESSION   DASWAV010000538 DASWAV010000000
VERSION     DASWAV010000538.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960316
            Sequence Read Archive: SRR10260007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 3072)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3072)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:47:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,929
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3072
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_109719"
                     /isolate="SMAG_U2665"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.36444 N 132.31889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1671)
                     /locus_tag="VF958_11240"
     CDS             complement(<1..1671)
                     /locus_tag="VF958_11240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9149724.1"
                     /translation="MSRRGRAFLSGLAVVVLFSLPLLPEIAGSRRLVFRDAHMTHWPW
                     RRVAMQMLDSGKVPFVNETASGGQPFLANPNAVLLYPTLLLEKILPPAAAFNLHYLLH
                     VLWAFFGARALASRFGLSGGAAFFSGIAFAFSGTMLSYGSAFANAGPAASWLPWCAAA
                     TIDIVRAGSGKRLLAAGAAAGLAFGLQLLAGEPALSLLTLFFCAMLAVAGWLGTPGAR
                     GKRGLEVAAGGLLAGGIAAAIAAPLLVPLAEIVRLTYRGQHQYSARAFGASPFALWRM
                     VEWLFPRFNGDPGTLGAGAHWQYRLHDGEIIYIWCVSFGVLPLLCIVLGAARRDFWSP
                     KTWLLAGAALVTLALSFGFAFPLYRFLFSVELLRRFRYPIKFYLLTTLCVALLAGFAV
                     DALKRRRAGRVEALLLGVALAVYAAGFAAAASGGALDALVRPELSGLTLSAELILPAI
                     RRVFRGDALLGLAAVGLLAVFVFAWRPRRGSVYAIGVGALVLALPSGLPLFVSGVQKD
                     LARPPALRGYLAGTGRLYASPELASLAVLETRITRPDLAPTVAKLARAQ"
     gene            1711..>3072
                     /locus_tag="VF958_11245"
     CDS             1711..>3072
                     /locus_tag="VF958_11245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9149725.1"
                     /translation="MSRRARPSPARLACAVVVGAFAFFCAWFTGFYPPFSNPNELSRF
                     QTVVAFVETGSFAIDSQLATLGDHEDKAAARGRFYSNKAPGLAFAAIPVYRLLRVAFP
                     PPDSPTAPVLFLLRLFTVSLVCVVALLRLARRMLLSREGESAAALVTFAVAFGTPFLY
                     YARSFFAHAWTASLVFLAWDVLRESAAAASPGGRRARLAAAGFLAGWALISEYTVAPV
                     VLALAVRAAVFPGRRSGANVLSFAGGAALPVGLLLAYQAACFGSPFLPSYAREAYPAY
                     AELAARRFFGFGLPSPAVALAYLFHPARGILLFSPFFLWAGVGAARWWRDRRERADGI
                     LAVVATAVTFLLLTGYPNWHGGWALGSRYLLPLLFLPAAALPLALATPLSRGLFAAAV
                     VFAVGVHLLATSSWPNFPLDFTFPPASGSAWFLARGWVAPNIGAGVGLPAAISLGIPL
                     AIAL"
BASE COUNT          448 a         1081 c         1091 g          452 t
ORIGIN      
        1 ctgggcccgg gcgagcttcg ccacggtcgg cgcgagatcc gggcgggtga tccgggtctc
       61 gaggacggcg agagaggcga gctcgggaga tgcgtacagc cggccggtcc ccgcgagata
      121 cccgcggagg gccggcgggc gcgcgagatc cttctggacc ccggagacga agagagggag
      181 acccgacgga agagcgagga cgagcgcgcc cactccgatc gcgtagacgc ttccgcgccg
      241 ggggcgccac gcgaagacga agacggcgag cagtccgacg gcggcgagac cgagaagcgc
      301 gtccccgcgg aagacgcggc ggattgcggg cagaatcagc tccgcggaca gcgtcaggcc
      361 ggaaagctcc ggccggacca gcgcgtcgag cgcgcctccg gacgccgccg cggcaaatcc
      421 cgcggcgtag acggcgagcg cgactccgag gaggagcgcc tcgacccggc ccgcccgacg
      481 ccgcttgagc gcgtccaccg cgaaacctgc gaggagtgcg acacagagcg tcgtcaggag
      541 gtagaacttg atgggatatc ggaatcggcg caacagttcg acggagaaga gaaaccggta
      601 cagcgggaag gcgaacccga acgagagggc gagcgtgacg agcgccgcgc ccgcgagcag
      661 ccacgtcttg ggggaccaga agtcccgccg cgcggcaccg agcacaatgc acaggagcgg
      721 gagcacgccg aagctgacgc accagatgta gatgatttcc ccgtcgtgga gccggtactg
      781 ccagtgggca ccggcgccga gcgtccccgg atcgccgttg aaccgcggga agagccactc
      841 gaccatccgc cagagcgcga agggcgacgc tccgaaggcg cgcgcggaat attggtgctg
      901 cccgcgatag gtcaaccgga cgatctcggc gagcggcacg agcagcggag ccgcgatcgc
      961 ggccgcgatg cctcccgcca ggagtcctcc cgccgcgacc tcgagtcccc gctttccgcg
     1021 cgcgccgggc gtcccgagcc atccggccac ggcgagcatc gcgcagaaga agagcgtcag
     1081 gagcgagaga gcgggttcgc cggcgaggag ttgaagaccg aaggcgagac cggcggccgc
     1141 gccggccgcc aggagcctct tcccggagcc ggcgcggacg atgtcgatcg tggcggccgc
     1201 gcaccagggc agccaggagg ccgcgggtcc ggcattggcg aaggcggagc cgtaagacag
     1261 catcgtcccg gagaatgcga acgcgatccc cgagaagaac gccgctccgc cggaaagccc
     1321 gaaccgggac gcgagcgcgc gcgcgccgaa gaacgcccag agcacgtgca acaggtagtg
     1381 cagattgaag gcggcggccg gcggcaggat cttttccagg agcaacgtgg gatagagcag
     1441 cacggcgttc gggttcgcga ggaacggctg tccgcccgac gcagtctcgt tgacgaacgg
     1501 gaccttgccg gagtcgagca tctgcatcgc gacgcggcgc cagggccagt gcgtcatgtg
     1561 ggcgtcgcgg aagacgaggc ggcgcgagcc cgcgatctcc ggcaagagcg gcagggaaaa
     1621 gaggacgacg accgcgaggc ccgagaggaa ggcgcggcct cggcgcgaca tgcggcgatt
     1681 gtaagcgcgc ggcgccgcta caattcgtcg atgtctcgac gcgcaagacc atccccggcc
     1741 cgtcttgcgt gcgcggtcgt ggtgggcgcg ttcgccttct tctgcgcctg gttcaccggt
     1801 ttttatccgc cgttctccaa tcccaacgag ctctcgcgct ttcagaccgt cgtcgctttc
     1861 gtcgagacag gctccttcgc gatcgattcc cagctggcga cgctcggcga tcacgaggac
     1921 aaggcggcgg cgagggggcg cttctactcg aacaaggcgc ccgggctcgc gttcgcggcg
     1981 atccccgtct atcgtctcct tcgggtcgcg tttccgcccc ccgattcgcc gaccgccccc
     2041 gtgctcttcc tgctccgcct cttcaccgtt tcgctcgtct gcgtcgtcgc gctcctgcgc
     2101 ctcgcgcggc gcatgctcct ctcgcgcgag ggggagagcg ccgcggcgct cgtgaccttc
     2161 gccgttgcgt tcggaactcc gttcctctac tacgcgcggt cgttcttcgc ccacgcgtgg
     2221 accgcgtcac tcgtcttcct ggcgtgggac gtcctgcgag agtccgcggc ggccgcgtcg
     2281 ccggggggac gccgcgcccg gctcgctgcg gccggatttc tcgcgggctg ggcgctcatc
     2341 tccgagtaca cggtggcgcc cgtcgtcctc gcgctcgcgg tccgcgccgc ggtgttcccc
     2401 ggtcggcgct ccggcgcgaa cgtcctgtcg tttgcgggcg gcgccgccct tcccgtgggt
     2461 ctcctcctcg cgtaccaggc cgcctgcttc ggatcgccgt tcctgccgtc gtacgcgcgc
     2521 gaggcatatc ccgcgtacgc ggagctggcg gcccgccggt tcttcgggtt cggactcccg
     2581 agtccggccg tcgcgctcgc ctacctcttc catcccgccc gcgggatact ccttttctcg
     2641 cctttcttcc tgtgggcggg ggtcggagcc gctcggtggt ggcgggaccg gcgggagcgc
     2701 gcggacggaa tcctcgcggt cgtcgcgacc gccgtgacgt tcctgctcct gaccggttat
     2761 ccgaactggc acggaggctg ggcgctcggg agccggtacc tcctgcccct tctcttcctc
     2821 ccggcggcgg cgctgccgtt ggcgctcgcg accccgctct cgcgcggcct cttcgccgcc
     2881 gcggtggtct tcgccgtcgg cgtgcacctt ctcgcgacgt cctcctggcc gaacttcccg
     2941 ctcgacttca cgttccctcc ggccagcgga tcggcctggt tcctcgcgcg cggatgggtg
     3001 gctcccaata tcggggcggg cgtcggttta ccggcagcga tctcgctcgg aattccactc
     3061 gcgatcgctc tc
//
LOCUS       DASWAV010000539        10801 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U2665
            k141_669089, whole genome shotgun sequence.
ACCESSION   DASWAV010000539 DASWAV010000000
VERSION     DASWAV010000539.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960316
            Sequence Read Archive: SRR10260007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 10801)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10801)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:47:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,929
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10801
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_669089"
                     /isolate="SMAG_U2665"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.36444 N 132.31889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..646
                     /locus_tag="VF958_11250"
     CDS             <1..646
                     /locus_tag="VF958_11250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014428680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HEX9149726.1"
                     /translation="PETLVESELFGHVRGAFTGATGSRPGRFELAQGGTLFLDEITEM
                     GSELQVKLLRVLEDRTLRRVGGSQELKVDVRLISATNRDPHDAIKEKKLREDLYYRLN
                     VFPLALPTLIERREDISLLAEHFRKSIEDQERAGVSSWDPRALELLQAYEWPGNVREL
                     RNIVHRAYVMTEGKTIRPEVVASLLPRSARPAAVVAKKKTALKQRPAAAAGRKK"
     gene            752..1141
                     /locus_tag="VF958_11255"
     CDS             752..1141
                     /locus_tag="VF958_11255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9149727.1"
                     /translation="MQMTPAAAHYVLQKLISQGRVRESQVRAVLNGLSQEKAALRERL
                     AALEGLDGRGARRGGRRSGGATRGGARRRRVAISPKVRALRRLQGKYMGYVRRLKPAE
                     KARVRAVREKQGMEAAIRLASSLGKSS"
     gene            1243..1566
                     /locus_tag="VF958_11260"
     CDS             1243..1566
                     /locus_tag="VF958_11260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9149728.1"
                     /translation="MADRKTDAPESSRSSRPSDTGKKRLKPEQYELKVRWETARQVIE
                     VTPTGVRFDFNTALKVGTRYPVSLKAPGVSLSTTLEVSRCQLTVDSAAGRFFRVTGKF
                     YPYVE"
     gene            complement(1535..1801)
                     /locus_tag="VF958_11265"
     CDS             complement(1535..1801)
                     /locus_tag="VF958_11265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9149729.1"
                     /translation="MLVWREHRGGSRSVFEGAVKRLEPDGVLWIVTASKKVRGPATPA
                     VHRLELSDLVKAFSKEGLTQDREFRVTPWHMAYRFTRRTGRTFP"
     gene            2083..2232
                     /locus_tag="VF958_11270"
     CDS             2083..2232
                     /locus_tag="VF958_11270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012465865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lmo0937 family membrane protein"
                     /protein_id="HEX9149730.1"
                     /translation="MLWTIFIILLVLWLLGLISGYTIGGFIHVLLVIAVVVLIIRLIQ
                     GRPVA"
     gene            complement(2311..2730)
                     /locus_tag="VF958_11275"
     CDS             complement(2311..2730)
                     /locus_tag="VF958_11275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9149731.1"
                     /translation="MSAETTGPRCPRCRRLIAAWRLAHCVYCGEPFPPDLKEGHAEPE
                     ALKWVERPSIPSDAAKQLELMKFFPGEVKKAPRSRPILIGIGAVSLVTFAVVFVLLYL
                     LLRRSAPALGAFVLVLGAGFLAYLGWVFLRADRRGSL"
     gene            2806..3858
                     /gene="asd"
                     /locus_tag="VF958_11280"
     CDS             2806..3858
                     /gene="asd"
                     /locus_tag="VF958_11280"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015896596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004073 - aspartate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HEX9149732.1"
                     /translation="MKTKIPVGVLGATGTVGQRFVERLASHPWFELVSVAASERSAGR
                     PYGEATRWRLASSLPERIASMEVRDVADELPCRAVFSALDAVVAREVEPLFARRGAFV
                     FSNASAHRMDDDVPLVIPEINPDSLCLLERQRASRGWKGSLVTNANCSAMFLTMALAP
                     LDRAFGVEKAFVSTMQAVSGAGYPGVPSLDILGNVIPDVPGEAEKIEREVAKILGGAA
                     RGDGPPARLTISAMTYRVPVEDGHTEAVFVGLARKASANDVEAAWEDFRGRDEVRGLP
                     SAPERPIVYVRERQRPQPRRDCGAGDGMSTTVGGLQSCRLLDWKFTAMGHNTIRGAAG
                     ASILNAELAVARGLLP"
     gene            3858..5204
                     /gene="lysC"
                     /locus_tag="VF958_11285"
     CDS             3858..5204
                     /gene="lysC"
                     /locus_tag="VF958_11285"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR00657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine-sensitive aspartokinase 3"
                     /protein_id="HEX9149733.1"
                     /translation="MVQKFGGTSLADAPALERVCAIVARARGECPIVVVSAIGDTTDQ
                     LIEALERAAAGDEARALTILSRLRRETGRVVKDFFGDAAPGIEKEIAPFFDELSRMAR
                     AVAVLRSVPPAGRDHFLAHGEMTSARLVARALAARSLPAVGVDSREILVTDDRFGRAQ
                     PDLDETGRRVAERLRAICDRGEIPVAAGYIGASHAGATTTLGRGGSDYTAALFAAALD
                     ADAVEIWTDVDGMMTADPRVVPAARPIETISFEEASELAYFGARVLHPLTLAPAIEKG
                     IPIRVKNSLSPAFPGTEIRASAPPSGERVRSIAFKKGIATVDVVTTRMLMAAGFLRTL
                     FEVFARYETPVDMVTTSEISVSVTVDDVSRLPEIRHELERLAAVEVGRDRAIVCLVGS
                     DIKFTPGIAARIFRTIEDVNVLMISQGASRRNVSFVVEERDVERVVRKLHEEFFER"
     gene            5224..5913
                     /locus_tag="VF958_11290"
     CDS             5224..5913
                     /locus_tag="VF958_11290"
                     /inference="COORDINATES: protein motif:HMM:NF017024.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008839 - 4-hydroxy-tetrahydrodipicolinate
                     reductase [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate reductase C-terminal
                     domain-containing protein"
                     /protein_id="HEX9149734.1"
                     /translation="MRFGIVGAGKMGCEIEAMAGGRGHDVVWKLDSGSNAGGAGLTAE
                     RLSRADVVFEFTNPAAAPSNLEALARAGAAVVCGTTGWDRDLTRIADAFRTGGGALVY
                     AANFSVGVRHFFELAKLAATLYPPAGYEPFLVEEHHSEKRDAPSGTAKRIAQIVEDAS
                     GKRPPVSSVRAGTIPGTHRLVFESPEDEVELIHRARGRGGFAKGAVWAAERIAGRQGV
                     FEFGELLREVR"
     gene            5922..6833
                     /gene="dapA"
                     /locus_tag="VF958_11295"
     CDS             5922..6833
                     /gene="dapA"
                     /locus_tag="VF958_11295"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013581328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008840 - 4-hydroxy-tetrahydrodipicolinate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HEX9149735.1"
                     /translation="MYRVDWKGCGTALVTPFDEKGRIDFRALAKLVDWQISQGIDFLV
                     PCGTTGESATLSGDERKAVTAAVVKAADGRVPIVAGAGGNHTAKAVFWARDAEEVGAD
                     GILSVSPMYNKPSPDGLVRHFEAIAEASDLPIVAYNVPTRTGTDMDVETILRIAEIPR
                     VAALKEASPNFGKIARLMATIPEDFVVFSGEDSTALALIALGGRGLISVASNEIPSEM
                     SALCAAALSNEWDEARRLQRKYLPLMEMNFWESSPGPAKCALAAMGRCGDTVRLPLVT
                     VRDETRRRIEKLLNGLKLIPKKAAAGR"
     gene            6846..7688
                     /locus_tag="VF958_11300"
     CDS             6846..7688
                     /locus_tag="VF958_11300"
                     /EC_number="2.3.1.117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011522889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase"
                     /protein_id="HEX9149736.1"
                     /translation="MHDVGGLARAIETAAANPDRASPGDLRRLFDELKAGLNDGSIRA
                     AEKEGERWVARPWVKKGILLGFRVGSLVRMAEGAGFPFFDKDTFPVKDLAADAGVRIV
                     PGGTSVRDGCYVAPGVTIMPPSYVNVGAWVGEGTMLDSHVLVGSCAQIGRRVHVSAGA
                     QIGGVLEPVGELPVIIEDGVLVGGNCGIYEGAIIRERAVLASGVIVTGGTPVYDVVRG
                     EVLRKEPGRPLEIPAGAVVVPGSRAIRTGKGSEWGLSLQTPVIVKYRDAKTDASATLE
                     DLLR"
     gene            7706..8056
                     /locus_tag="VF958_11305"
     CDS             7706..8056
                     /locus_tag="VF958_11305"
                     /inference="COORDINATES: protein motif:HMM:NF024845.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-TFIIB domain-containing protein"
                     /protein_id="HEX9149737.1"
                     /translation="MPWDSEEIRRGRENDWFRQNEEKLIEAARKRREQAAREGQSAEA
                     QARRELHWRKCPKCGNDMHVETSSGIEIEKCSSCEGIFFDRGELEQLLVKHDSERRGF
                     FRRLLGFGEENPKP"
     gene            complement(8067..9440)
                     /locus_tag="VF958_11310"
     CDS             complement(8067..9440)
                     /locus_tag="VF958_11310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009382981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HEX9149738.1"
                     /translation="MAIVFTNEELKKYAATKRDEYEDDLKKIVEIPTISVEPERKGDV
                     HRGAEFVASLLESAGAKVRLYETKGHPIVYGRFDRGPNVPTVTVYNHLDVQPAEGDDW
                     LTAPFEFVKKGNRYFGRGTTDDKGPAMTAFYGARYAWEHDVPLNIHLLWEFEEEIGSP
                     NFEPTIRANAREFATDSVVVVDTVWVSRSRPAQPAGLRGLQGFRFTLQTGETDQHSGT
                     AGGAARNPVTELCQLISECVDGRTGRVKIPGFYSDVIPPSKKDLEDLKNSGFTTRDFK
                     RDHLFRSLRVDDAMEIMKRVWMMPTFEVHGIAGGYQGPGVKTIIPPKATAIVSCRLVP
                     KMDPKKVVRLVTDFVKQKNPDVKVASEHALPAYQGKTTGPYADAIRASMKFAFGKEPV
                     FVREGGSIGAVLSMEKVFKAPVFFLGLSLPEHGYHAPNENYDWGQAEGGMAAFADYFR
                     RVAEIPA"
     gene            complement(9488..10090)
                     /locus_tag="VF958_11315"
     CDS             complement(9488..10090)
                     /locus_tag="VF958_11315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9149739.1"
                     /translation="MSLLYLHFESDERAGRVQKLAKSGAHVIVAEPKWPGFWEIAKRE
                     KPMAIAVDFTHAPSHALETADYISKARETRETPIYLLRVPEDRIEMVQKRLPQAGIVT
                     EPELAERLAAMEREAAARALEKKEAAAVARRSAAAAKQAAKKAAAGGKTPAKKTKAAA
                     PSKSEPAGARKKSPSPPKKKNVVKPASKKPKSSRPARKNK"
     gene            complement(10103..>10801)
                     /locus_tag="VF958_11320"
     CDS             complement(10103..>10801)
                     /locus_tag="VF958_11320"
                     /inference="COORDINATES: protein motif:HMM:NF013086.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HEX9149740.1"
                     /translation="ERDEPYAYLDISHKPADWIRERFPGLTKRCLEYGFDLTAGPIPV
                     VPAAHYSCGGVAVDLDGATTLAHLRAAGEVACSGVHGANRLASTSLLEGLLWGWRSGK
                     AAVAAIRSEPLRRRVEVRPWEPEFEPVDPALIAQDWLVVRHTMWNYVGLLRSEKRLDR
                     AGRILAELRDEIENFYRRGTMSDELLGLRNGIQTALAIHQAASDDHVSRGTHYREDSA
                     EAISSPRRKRATLL"
BASE COUNT         1743 a         3554 c         3674 g         1830 t
ORIGIN      
        1 tcccgagacc ctggtggaga gcgagttgtt cggccacgtc agaggcgcat tcaccggggc
       61 taccggatca cggcccggga gattcgaact ggcgcagggc gggactctct tcctggacga
      121 gatcacagag atggggtcgg aactccaggt gaagctcctc cgcgttctcg aagaccgcac
      181 actgcggcgc gtcggaggtt cgcaggagct caaggtcgac gtccggctga tctccgcgac
      241 caaccgcgac ccccacgacg cgatcaagga gaagaagctt cgcgaagacc tctattaccg
      301 gttgaacgtg tttccgctcg cgcttccgac gctcatcgag cgccgcgaag acatttcgct
      361 gctcgccgag catttccgga aatcgatcga ggaccaggag cgggcgggcg tgtcgagctg
      421 ggaccccagg gcactcgaac ttctgcaggc atacgagtgg cccgggaacg tgcgcgaact
      481 gcgcaacatc gtgcaccgcg cctatgtcat gacggaggga aagaccattc gtccggaggt
      541 tgttgcgagt ctccttccgc gctccgcccg tccggccgcg gtcgtcgcga agaagaagac
      601 ggccctcaaa caaaggcccg cggccgcggc ggggcgtaag aaataggact ccattgttgc
      661 gctttgatca cgctttcgcg cttgttgtag actgccgcgc gccgcaaaca ggcggccctt
      721 caatgaattt cactatgagg ccattcaaac gatgcaaatg acgccggccg cagcgcatta
      781 cgttcttcag aaactgattt cccagggaag agtccgtgaa tcccaggttc gcgccgtcct
      841 gaacgggctg tcgcaagaga aagccgcgct gcgcgagcgg ctcgcggcgc tcgaaggttt
      901 ggatggtcgc ggcgcgcgcc gcggtggacg tcgaagcggc ggcgctaccc gcggaggtgc
      961 acggcggcgc cgcgtggcga tctctccgaa ggttcgcgcc ctccgacgac tccagggcaa
     1021 gtacatgggc tacgtccgac gactgaagcc cgcggagaag gctcgcgtcc gggcggtgcg
     1081 ggaaaagcag gggatggagg ccgcaatccg gctcgcgtcc tcgctcggaa aatcttcgta
     1141 aaaaaaagct ggctccggta agctcgtggg gcaaacgagc tggcgtaggt ttcgctagac
     1201 ctggagggcg tggacaactc ggatccttcc cgatttcttc caatggccga tcgcaagacc
     1261 gacgcgcccg agtccagccg ctcatcccgc ccgagcgata ccgggaagaa gcgtctgaag
     1321 cccgagcaat acgagctcaa ggttcgctgg gagaccgcgc gccaggtgat cgaggtcact
     1381 ccgacgggag tgcgcttcga cttcaacacg gcgctcaaag ttggaacgcg ctatccggta
     1441 agcctgaagg caccgggggt ctctctctcg acgacgctgg aagtttcgcg atgccagctc
     1501 accgttgact ccgcggcggg gcgattcttt cgcgttacgg gaaagttcta cccgtacgtc
     1561 gagtgaatcg ataggccatg tgccacgggg tcacgcgaaa ttcgcggtcc tgcgtgaggc
     1621 cctccttcga gaacgctttg acgagatcgg aaagctccag acggtgaacg gccggcgtgg
     1681 ccggcccgcg caccttcttc gacgcggtca cgatccacaa aaccccgtcg ggctcgagtc
     1741 gcttcaccgc gccttcgaag accgaccgtg acccgccgcg gtgctcgcgc cacacgagca
     1801 ccgcgtcaaa cgcgttcttg acggcccgga tcgcatcgcc actcgtcgaa agcgtcgtgc
     1861 gcggctcccc cgcgcgctct cgaagagccg agcgagttcg tccggggcgt cgacgagcag
     1921 cagtcgctcg ggcgcctcca gctccatcct cgccacgagc tcgccgggat tcttgagatt
     1981 caccacttcc cgtctctgcg gactcgagga attggcacga ggttcgctgt agacaccccg
     2041 ggctgcctac ggtagctcgg tcgaatagga ggagacgaca tcatgctttg gaccattttc
     2101 atcatcctgc tggttctctg gctcctgggt ttgatctcgg gctacaccat cggcggcttc
     2161 attcacgtcc ttctcgtcat cgcggtcgtc gttctgatca tccggctgat tcaggggcgc
     2221 ccggtcgcct gaccatcggg ttccgcttct ccgacgagcc ccgcgaccag gggctcgtcg
     2281 tttttccggc tccgaaacgg gcgccgggcc tcagagcgag ccgcgccggt ccgcgcggag
     2341 aaagacccag ccgaggtagg ccaggaagcc ggctcccagc acgaggacga acgcgccgag
     2401 agcgggagcc gaacgcctca ggagcaggta gagcaggacg aaaaccacgg cgaacgtgac
     2461 cagtgatacc gcgccgatgc cgatcaggat cggcctcgag cgcggggcct tcttcacctc
     2521 ccccgggaaa aacttcatca gctcgagctg cttcgccgcg tccgagggga tcgacggcct
     2581 ttcgacccac ttgagcgcct caggctcggc gtgcccttct ttcaagtcgg gcggaaacgg
     2641 ctcgccgcaa taaacgcagt gcgcgagccg ccaggcggcg atcagccgcc ggcagcgagg
     2701 acatcgtggt ccggtcgtct cggcgctcat tccgtgaaat ttacaggaaa tgctggtcgt
     2761 gcggagaccg accgactatc ccggcaacgt tagactcgac gagtcatgaa gacaaagatc
     2821 cccgtcggcg tgctcggcgc gacgggcacg gttggtcaac gcttcgtcga acggctggcc
     2881 tcgcatccct ggttcgagct cgtctcggtc gcggcgtcgg agagatcggc gggtcgtccg
     2941 tacggggagg cgacccgctg gcggctcgct tcctctctcc cggagcgaat cgcctcgatg
     3001 gaggtccgcg acgtggcgga cgagctgccg tgccgagccg tattttcggc actcgacgcg
     3061 gtggtcgcca gggaggttga gccactcttc gcgcggcgcg gagctttcgt cttctcgaac
     3121 gcgtcggcgc accggatgga cgacgacgtg cctctcgtga tcccggaaat caatccggac
     3181 tcactctgcc ttctcgaacg ccagcgcgcg agccgagggt ggaaggggtc gctcgtcacg
     3241 aacgcgaact gctcggcgat gttcctgacg atggcgctgg cgccgctcga tcgcgcgttc
     3301 ggcgtcgaga aggcgttcgt ctccacgatg caggccgttt ccggcgcggg atatccgggc
     3361 gtcccctcgc tcgacatcct cggcaacgtg attcccgacg tcccggggga agcggagaag
     3421 atcgagcgcg aggtcgcgaa gatcctggga ggcgccgcgc ggggagacgg gccgccggcg
     3481 cggctcacga tctccgcaat gacctatcga gtgccggtcg aagacggcca cacggaggcg
     3541 gtcttcgtgg ggctggcgag gaaggcttcc gcgaacgacg tcgaggcggc gtgggaggac
     3601 ttccgcggtc gcgacgaggt gcgtggtctc ccgtcagccc ccgagcgccc gatcgtctac
     3661 gtccgcgagc ggcagcggcc gcagccgcgg agggactgcg gagccggcga cggcatgtcg
     3721 acgaccgtcg gcggtctgca gtcctgccgc ctcctcgact ggaagttcac cgcgatgggc
     3781 cacaacacga tccggggagc cgcgggagcc tccatactga acgccgagct cgcagtcgcg
     3841 agaggactgc tgccctgatc gtccagaaat tcggcggcac gtcgctcgcc gacgcgcccg
     3901 ccctcgagcg cgtctgcgcg atcgtcgccc gtgcgcgggg ggagtgcccg atcgtcgtcg
     3961 tgtcggcgat cggggatacg accgaccagc tgatcgaagc gctcgagcgc gcggcggcgg
     4021 gggacgaggc gcgtgcgctg acgatcctct cccgattgcg ccgcgagacc gggcgtgtgg
     4081 tgaaggattt cttcggtgac gccgctccgg ggatcgagaa ggagatcgcg cccttcttcg
     4141 acgagctgtc ccggatggcg cgcgccgtcg ctgtgctgcg atccgtaccg ccggcgggtc
     4201 gtgatcactt cctcgcgcac ggcgagatga cgtcggcgcg tctcgtggct cgcgcgctcg
     4261 ccgcgcggtc gctcccggcg gtcggcgtgg actctcgcga gatcctcgtc acggacgacc
     4321 ggttcggccg cgcccagccc gacctcgacg agacggggcg acgcgtggcc gagcgtcttc
     4381 gtgccatctg cgatcgggga gagatccccg tcgcggccgg ctacatcgga gcgagccatg
     4441 cgggcgcgac gaccacgctg ggacgcggcg gatcggacta cacggcggcg ctcttcgcgg
     4501 cggcgctcga cgccgacgcg gtcgaaatct ggaccgacgt cgacgggatg atgaccgcgg
     4561 atccgagggt cgtcccggcc gcccggccga tcgagacgat ctccttcgaa gaggcgtccg
     4621 agctcgccta cttcggcgcg cgcgtcctcc acccgctgac gctcgcgccg gcgatcgaaa
     4681 aggggatccc gatccgcgtg aagaactcgc tctccccggc ctttcccggg acggaaatcc
     4741 gcgcttcagc cccaccgtcc ggcgagcgcg tccggtcgat cgctttcaag aaagggatcg
     4801 cgaccgttga cgtggtgacg acccgcatgc tcatggccgc cgggttcctc cggaccctct
     4861 tcgaggtctt cgcgcggtac gagacccccg tcgacatggt gacgacgtcg gagatctccg
     4921 tctcggtgac cgtggacgac gtctcccgcc tgccggagat ccggcacgaa ctcgagcgtt
     4981 tggcggccgt cgaggtcggc cgcgatcgtg cgatcgtctg tctcgtcggg tcggacatca
     5041 agttcactcc cgggatcgcg gcccgcatct tccggacgat cgaggacgtg aacgtcctga
     5101 tgatttccca gggcgcctcg cgccggaacg tgtcgttcgt ggtcgaggag cgtgacgtcg
     5161 agcgggtcgt gcgcaagctg cacgaggagt tcttcgagcg ctgaaaactg atagcgtctc
     5221 gacatgcgct tcggcatcgt cggagcgggc aagatgggct gcgagatcga ggccatggcc
     5281 ggggggcgcg gacacgacgt cgtgtggaag ctcgactcgg gctcgaacgc cggcggagca
     5341 gggctgacgg cggagcgcct ctcgcgagcg gacgtcgtct tcgagttcac gaatccggcg
     5401 gccgcgcctt cgaacctcga ggccctggcg cgggcgggcg ccgcggtcgt ctgcgggacc
     5461 acggggtggg accgcgatct gacgcggatc gccgacgcct tccgcaccgg cgggggcgcg
     5521 ctcgtgtacg ccgcaaactt ctccgtcggc gtgcgtcact ttttcgagct cgcgaagctc
     5581 gcggcgactc tctatccccc cgccggctac gagccgtttc tcgtcgagga gcaccactcc
     5641 gaaaaacgcg acgccccctc gggcacggcg aagaggatcg cgcagatcgt ggaggacgcg
     5701 tccggaaaga ggccgccggt ctcgagcgtg cgcgccggca ccattcccgg gacacaccgt
     5761 ctcgtcttcg agtctccgga ggacgaggtg gagctcatcc accgcgcgcg cggccgtggc
     5821 gggttcgcga aaggcgcggt ctgggcggcg gagcggatcg cgggccggca gggtgtcttc
     5881 gagttcggcg agctgttgcg ggaagttcga taggaggaaa cgtgtaccgc gtggactgga
     5941 aagggtgcgg gacggcgctc gtcacgccgt tcgacgagaa gggacggatc gactttcgcg
     6001 cgctggcgaa gctggtcgac tggcagattt cgcaggggat cgactttctc gtgccgtgcg
     6061 gaacgaccgg cgaatccgcg acgctgtcgg gcgacgagag aaaggcggtc accgccgcgg
     6121 tggtcaaggc cgcggacggt cgggtgccga tcgtcgcggg cgccggcggg aaccacacgg
     6181 ccaaggccgt cttctgggcg cgcgacgccg aggaggtcgg cgccgacggg attctctcgg
     6241 tctcgccgat gtacaacaag ccctcgcccg acgggctcgt gcgccacttc gaagcgatcg
     6301 cggaggcgtc cgacctgccg atcgtcgcgt acaacgtgcc gacgcggacg ggcacggaca
     6361 tggacgtgga gaccatcctg cgcatcgcgg agatcccgcg cgtcgcggca ctcaaggagg
     6421 cgtcgcccaa cttcggcaag atcgcgcggc tcatggcgac gattccggag gacttcgtcg
     6481 ttttttccgg cgaggattcg acggcgcttg cgctgatcgc actgggaggc cgcgggctca
     6541 tctcggtcgc ctccaacgag attccttccg agatgagcgc gctgtgcgcc gcggcgctgt
     6601 cgaacgagtg ggacgaggcg cgccgcctgc agcgaaagta cctccccctg atggaaatga
     6661 acttctggga gtcgagtccg ggaccggcga agtgcgctct cgcggcgatg ggtcgctgtg
     6721 gcgacaccgt tcgcctgccg ctcgtcaccg tgcgggacga gacccggcgc cgcatcgaga
     6781 agctgctgaa cgggttgaag ctcatcccga agaaggccgc cgcgggtcgc tgagtcccgc
     6841 tcacgatgca cgacgtcggg ggactcgccc gagcgatcga gacggcggcc gcgaatccgg
     6901 accgggcctc gcccggcgat ctgcgccggc tcttcgacga gctgaaggcg ggcctgaacg
     6961 acggctcgat ccgggccgcc gagaaggagg gggagcggtg ggtcgcgcgg ccgtgggtca
     7021 agaaggggat cctgctcggc ttccgcgtcg gctctctcgt ccgcatggcg gagggcgccg
     7081 gctttccgtt tttcgacaag gacacgtttc cggtgaagga cctcgcggcg gacgcgggcg
     7141 tgcggatcgt gccgggggga acgtccgtcc gcgacggctg ctacgttgct cccggcgtga
     7201 cgatcatgcc gccgtcctac gtcaacgtcg gcgcgtgggt cggcgaaggg accatgctcg
     7261 actcacacgt gctcgtcggt tcgtgcgccc agatcgggag acgcgtgcac gtttcggcgg
     7321 gcgcgcagat cggcggcgtg ctcgagccgg tcggagagct tccggtgatc atcgaggacg
     7381 gggtgctggt cggcggcaac tgcggcatct acgagggcgc gatcatccgg gagcgcgcgg
     7441 tcctcgcctc gggggtgatc gtgaccggcg gtacgcccgt ctacgacgtc gtccggggag
     7501 aagtcctccg caaggagccg ggccgccccc tcgagatccc cgccggcgcc gtggtcgtgc
     7561 cgggctcgag agcgatccgc accggcaagg ggtccgagtg gggcctctcg ctccagacac
     7621 cggtgatcgt caagtaccgg gatgccaaga cggacgcctc ggctaccctg gaagatctgc
     7681 tccgatgacg gcttagaatc tctccatgcc ctgggatagc gaagagatcc gtcgcgggcg
     7741 cgagaacgac tggttccgcc agaacgagga gaagctgatc gaggccgcgc gaaagcggcg
     7801 cgagcaggcc gcgcgagaag gccagagcgc cgaagcgcag gcacgccgtg agctccactg
     7861 gcgcaagtgc cccaagtgcg gcaacgacat gcacgtcgag acgagctccg gcatcgagat
     7921 cgagaagtgc tcctcctgcg aaggcatctt cttcgaccgc ggagagctcg agcagctgct
     7981 cgtcaagcac gacagcgaac gccgcggctt cttccgtcgg cttcttggct tcggcgagga
     8041 gaacccgaag ccctgagtcc gaggtcctac gccgggattt ccgcgaccct ccggaagtag
     8101 tcggcgaacg ccgccatccc gccttcggcc tggccccagt cgtagttctc gttcggtgcg
     8161 tggtagccgt gctcgggtaa tgacagcccc aggaagaaca cgggagcctt gaacaccttc
     8221 tccatcgaga gcacggcgcc gatcgacccg ccttcgcgta cgaaaacggg ctccttgccg
     8281 aaagcgaact tcatcgacgc ccggatcgcg tccgcgtaag ggcccgtcgt cttgccctgg
     8341 taggccggga gcgcgtgctc cgaagcgacc ttcacgtccg ggttcttctg cttcacgaaa
     8401 tcggtcacca gtcggacgac ctttttcggg tccattttcg gaacgaggcg gcacgagacg
     8461 atcgccgtcg ccttcggcgg gatgatcgtc ttgacccccg gaccctgata tccaccggcg
     8521 attccgtgca cctcgaacgt cggcatcatc cagacgcgtt tcatgatctc catggcgtcg
     8581 tcgacgcgaa gcgaccggaa gaggtgatcg cgcttgaagt cccgcgtcgt gaagcccgaa
     8641 ttcttcagat cttcgaggtc cttcttcgac gggggaatca catccgaata gaagcccggg
     8701 atcttcacgc ggcccgtgcg gccgtcgacg cactcggaga tgagctgaca gagttcggtg
     8761 accggattcc tcgcggcgcc gccggccgtg ccggagtgct ggtcggtctc ccccgtctgc
     8821 agcgtgaatc gaaagccctg cagtccccgc aggcccgccg gctgcgccgg acgggagcgc
     8881 gagacccaca cagtgtcgac gacgacgacc gaatccgtcg cgaattccct ggcgttcgcc
     8941 ctgatcgtcg gctcgaagtt cgggcttccg atctcctcct cgaactccca caagaggtga
     9001 atgttcagcg ggacgtcgtg ctcccaggcg taacgcgcgc cgtagaacgc cgtcatcgcc
     9061 ggtcccttgt cgtcggttgt cccgcgaccg aaataccgat tgcccttctt cacgaactcg
     9121 aaaggggcgg tgagccagtc gtcgccttcc gcgggctgga cgtcgaggtg gttgtaaacc
     9181 gtcaccgtgg gaacgttcgg gccccggtcg aagcggccat agacgatcgg gtggcccttc
     9241 gtctcataca accgtacttt cgcgcccgcc gactcgagca gagacgccac gaactcggct
     9301 ccgcggtgca catctccttt tcgctcgggc tccacagaga tcgtcgggat ctcgacgatc
     9361 ttcttgagat cgtcttcgta ctcgtcgcgc tttgtcgcgg cgtatttctt caactcctcg
     9421 ttcgtaaaca caatcgccat cggactcggc ctcccgtttc acaaacgccg attctatcgg
     9481 agggttctta cttgttcttc ctggctggcc gcgacgactt cggctttttc gacgccggct
     9541 tcacgacgtt cttcttcttc ggaggcgacg gactcttctt gcgggcgccc gcgggctcgg
     9601 acttcgaagg cgccgccgct ttcgtcttct tcgccggcgt tttcccgccg gcggccgctt
     9661 tcttcgcggc ctgtttcgcc gcagccgccg aacggcgcgc gaccgcggcc gcctccttct
     9721 tctcgagcgc ccgggccgcc gcttcgcgtt ccatcgcggc gaggcgctcg gcgagctccg
     9781 gctccgtgac gatgcccgcc tgcggaagcc tcttctggac catctcgatc cggtcttcgg
     9841 gcacgcggag gagatagatc ggcgtctcgc gggtctcgcg cgccttggag atgtagtcgg
     9901 ccgtctcgag ggcgtgcgac ggtgcgtggg tgaagtcgac ggcgatcgcc atcggctttt
     9961 cgcgcttggc gatctcccag aaccccggcc atttgggctc cgcaacgatg acgtgggcgc
    10021 cgctctttgc gagcttctgg acgcgcccgg cgcgctcgtc gctctcgaaa tgcaggtaca
    10081 gcaggctcat gcgaccgcga gactacaaca gtgtcgcccg tttgcgccgc ggcgaggaaa
    10141 tggcctcggc gctgtcctcg cggtagtggg tcccccgcga aacgtggtcg tcagacgcgg
    10201 cctggtgaat ggcgagcgcc gtctggatgc cgttgcgcag ccccaggagc tcgtcggaca
    10261 tcgttccccg gcggtagaaa ttctcgatct cgtcccgcag ctcggcgagg atgcgtcccg
    10321 cccggtcgag ccgtttttcc gaacgaagca acccgacgta gttccacatg gtgtgacgca
    10381 cgacgagcca gtcctgggcg atcagcgccg ggtcgacggg ttcgaactcc ggctcccagg
    10441 ggcggacctc gacgcgccgc cggagcggct cggaccggat cgccgcgacg gccgctttac
    10501 ccgaccgcca gccccagagc agtccttcga ggagcgacgt cgacgcgagg cggttcgcgc
    10561 cgtggacgcc gctgcaggcg acctccccgg ccgcgcgcag gtgcgccagc gtagtcgcgc
    10621 cgtcgagatc gacggcgacg cctccgcagg agtagtgagc ggcggggacg accgggatcg
    10681 gacccgcggt caggtcgaag ccgtattcga ggcaccgttt cgtgagaccc gggaaccgct
    10741 cgcgaatcca gtctgcgggc ttgtgggaga tgtcgagata cgcgtacggc tcgtcgcgct
    10801 c
//
LOCUS       DASWAV010000540         5087 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U2665
            k141_551371, whole genome shotgun sequence.
ACCESSION   DASWAV010000540 DASWAV010000000
VERSION     DASWAV010000540.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960316
            Sequence Read Archive: SRR10260007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 5087)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5087)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:47:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,929
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5087
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_551371"
                     /isolate="SMAG_U2665"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.36444 N 132.31889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1594
                     /locus_tag="VF958_11325"
     CDS             <1..1594
                     /locus_tag="VF958_11325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9149741.1"
                     /translation="SLALNNLLASPDFENWRQGDPLDPARLARGDSGRPACNLFYLAH
                     LDDRERMFFVSRFLDRFWSWARTQTGTTDLRALLYFDEVMGFLPPSAEPPSKRPMLSL
                     LKQGRAFGVGSLLVTQNPVDLDYKALTNAGTWLIGRLQAERDKERLLDGLESAGIGMS
                     RAEADRTISGLEKRRFLLHDVHRAGGPVVFETRWARAYLRGPLALRELPDLVALAGGS
                     EPVRGAASAPAPKASRSSDGAPVPPSLDPVFQHRYLSSTGGPLSGDIAVVVEARVNRQ
                     RPQVSGVRRALVRFGSGAAPVPTVDEPESLGKVTSDAPAAASYGPLPSWATKTNAAAA
                     LERLARDAVSAQGLDLENVPGLGLVRSPEETGEAFESRVKAAIESEVVKRTEKSRGPL
                     ARKLESLERKIEEENRELERDRAESARSKTYSAIDVGASILTSVLGGRRSSIGSAGRA
                     GARAYGRIQRTAENVKESEKRIAEWTSERDALSAELERVTASEREGVTAAAARRERIR
                     VPVNRADVRPLEWYVLWSRIGV"
     gene            1769..3013
                     /gene="clpX"
                     /locus_tag="VF958_11330"
     CDS             1769..3013
                     /gene="clpX"
                     /locus_tag="VF958_11330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014912902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HEX9149742.1"
                     /translation="MSKKGGNGDVLRCSFCNKSQRDVKKLIAGPTVYICDECVDICLD
                     IIAEDRVLETQAPETKLPKPKEIKEFLDGYVIGQEQAKKKLAVAVYNHYKRIDYLEKN
                     RKLDVELQKSNILLIGPTGTGKTLLAQTLAKLLSVPFTIVDATTLTEAGYVGEDVENI
                     ILKLYQAAGNDKDKTERGIVYIDEVDKIARKGENPSITRDVSGEGVQQALLKILEGTV
                     ANVPPQGGRKHPHQEFIQIDTTNILFLCGGAFVGLEDIISRRMTEKTMGFGAKITTKK
                     EKRSSVLLEKVHPEDLIKYGMIPEFVGRLPVVATLTELDQPALVEILREPKNSLVKQY
                     QTMLEFENVQLRFTDDAIEAIAEEALARNVGARGLKIIIEDLMLDVMYQIPSDEDIEE
                     CVITKDVVKHHTNPITILRKAG"
     gene            3087..>5087
                     /gene="lon"
                     /locus_tag="VF958_11335"
     CDS             3087..>5087
                     /gene="lon"
                     /locus_tag="VF958_11335"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013579797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HEX9149743.1"
                     /translation="MKSTDIATRTGETLPLVPLRDMVVFPHMMAPFVVGRDSSIRALE
                     AALATSGKRLFLSAQKDPQLDEPKPDDIHPMGVVATVAQSLKLPNGHIKVMVEGARRG
                     RIVGFEERAGYLAVSIEAVPTRSADVEELKEYMGKVLGLFEQYAKLSHHLAFEGLAAS
                     LKIDDPDRFADTLSAHLAVSTPEKQALLEAISPHERLQKLQDLLEVEIEKVNIDRRIN
                     NKVKKQMEKAQKEYYLNEKIKAIHQELGRKDDRTDEVDDLKKKLATSGMPKEVREKAE
                     QELRRLEAMPPVSAEATVSRNYIEWLVGVPWRKASRESKDLSRAEKILNEDHFGLEKV
                     KERILEFLAVRQLVQKTKGSIICFVGPPGVGKTSLAKSIARALNRKFVRLSLGGVRDE
                     AEIRGHRRTYIGAFPGQIIQMMKKAGTVNPVFLLDEVDKTSSDFRGDPASALLEVLDP
                     EQNHTFVDHYLDVEYDLSKVMFIATANVADPIPPALKDRMELIPLSGYTPNEKLAIAQ
                     KYLVSKQREANGLKPDQIDVSDEAILSLISNYTREAGVRNLEREIANVCRKVARKIVQ
                     KKVEPPVQVTGDNLRDFLGKHKFRNRRKGEVSEVGIATGLAWTEAGGEILTTETSLLK
                     GKGNLTLTGSLGDVMQESARAAVSYVRSRAEMYNADPNFHEKY"
BASE COUNT          998 a         1667 c         1651 g          771 t
ORIGIN      
        1 ctccctcgcg ctcaacaacc tcctcgcctc gccggacttc gaaaactggc gccaggggga
       61 cccgctcgat ccggcgcggc tcgcgcgcgg cgactccggc cgcccggcgt gtaacctctt
      121 ctacctcgct cacctcgacg accgcgagcg catgttcttc gtgtcgcgct tcctcgatcg
      181 cttctggagc tgggcccgca cgcagacggg cacgacggat ctgcgggccc tcctctactt
      241 cgacgaggtg atgggattcc tcccgccgag cgccgagcct ccgtcgaagc gcccgatgct
      301 ctcgctcctg aagcagggtc gcgcgttcgg agtcggatcg cttctcgtca cgcagaatcc
      361 cgtcgacctc gactacaaag cgttgaccaa cgcggggacg tggctcatcg gccgcctgca
      421 ggcggagcgc gacaaggaga gattgctcga tggcctcgag tcggcgggca tcgggatgtc
      481 ccgcgccgaa gccgatcgta cgatctccgg cctcgaaaag agacgattcc tcctgcacga
      541 cgtccatcga gcgggagggc cggtcgtctt cgagacccgc tgggctcgtg cgtacctgcg
      601 agggcctctc gcgctgcgcg agctcccgga cctcgtcgcc ttagccggtg ggagcgagcc
      661 cgtgcgcggc gccgcgagcg cgcccgcacc gaaggcaagt cggtcatccg acggcgcccc
      721 ggttccgccg tcgctcgatc cggttttcca gcaccggtat ctatcgtcga cgggtgggcc
      781 actctccggg gacatcgccg tagtcgtcga agcgcgcgtc aaccggcagc ggccccaggt
      841 gtcgggagtt cgccgcgctc tcgttcgatt cggatccggc gcggcgccgg tcccgacggt
      901 cgacgagccc gagtcgctcg gcaaggtgac ctccgatgca cccgccgccg cctcgtacgg
      961 tccgctcccc tcgtgggcga cgaagacgaa cgccgccgcg gcgctcgaac ggctcgctcg
     1021 ggacgccgtt tcggcgcagg ggctcgatct cgaaaacgtc cccgggctcg gtctcgtccg
     1081 cagtccggag gagaccggcg aggcgttcga gtcgcgcgtc aaggccgcca tcgagtccga
     1141 ggtcgtcaaa cggaccgaga agtcccgggg gccgctcgct cgcaagctcg agtcgctcga
     1201 gcgaaagatc gaggaggaga acagggagct cgaacgcgat cgcgccgagt cggcccgatc
     1261 caagacttac agcgcgatcg acgtcggcgc cagcatcctg acgagcgtgc tcggcgggcg
     1321 acgcagctcg atcggctccg cgggccgggc gggcgcgcgg gcctacggac ggatccagcg
     1381 caccgccgaa aacgtcaagg agtcggagaa gcgaatcgcc gaatggacct cggaacgcga
     1441 cgcgttatcg gccgagctcg agcgggtcac ggcgtccgag agggagggcg tcaccgcggc
     1501 ggcggcgcgg cgcgaacgaa tccgcgttcc ggtcaaccgc gccgacgtcc ggcctctgga
     1561 atggtatgtg ctttggtctc ggatcggcgt gtgacggacg gcactgatgc tgtcgtacca
     1621 ggcgtcgcgt tcgggagtgg aataggctga ccctgcggac gttaaggcgc gtgagggtgt
     1681 gctaaagtga actcaaaggg tggaagagag ctcgttgacg cgtccgcgaa agcgcgccta
     1741 gaatctcctt acccttcccg gaggcagaat gtcgaagaaa ggtggtaacg gcgacgttct
     1801 tcggtgttcc ttctgcaaca agagccagag ggacgtcaag aagctcatcg caggtcccac
     1861 ggtctacatc tgcgacgagt gcgtcgacat ctgtctcgac atcatcgccg aggaccgcgt
     1921 cctcgagacc caggcccccg agaccaagct ccccaagccg aaagagatca aggagttcct
     1981 cgacggctac gtgatcggac aggagcaggc gaagaaaaag ctcgcggtcg ccgtctacaa
     2041 ccactacaag cgcatcgact acctcgagaa gaaccggaaa ctcgacgtcg aactccagaa
     2101 gtccaacatc ctgctgatcg ggcccacggg gaccggcaag accctcctcg cccagaccct
     2161 cgcgaagctc ctctcggtgc cgttcacgat cgtcgacgcc acgaccctga ccgaggccgg
     2221 gtacgtcgga gaggacgtcg agaacatcat cctgaagctc tatcaggcgg ccggcaacga
     2281 caaggacaag acggagcgcg ggatcgtcta catcgacgag gtcgacaaga tcgcccgcaa
     2341 gggggagaac ccgtcgatca cgcgcgacgt gtcgggcgaa ggagtccagc aggccctcct
     2401 gaagatcctc gaggggaccg tcgccaacgt gccgccccag gggggccgca agcacccgca
     2461 ccaggagttc atccagatcg acacgaccaa catcctcttc ctgtgcggcg gggcgttcgt
     2521 tggtctcgag gacatcatca gccgccggat gaccgagaag acgatgggat tcggcgcgaa
     2581 gatcacgacg aagaaggaga agcgctcgag cgtcctcctg gagaaggtgc accccgagga
     2641 cctgatcaag tacgggatga tcccggagtt cgtcggccgc cttccggtcg tggcgaccct
     2701 gacggagctc gatcagcccg ctctggtcga aatcctgagg gagccgaaga actcgctcgt
     2761 gaagcagtac cagacgatgc tggaattcga gaacgtccag cttcgtttca ccgatgacgc
     2821 aatcgaggcc atcgccgagg aggctctggc ccgcaacgtc ggagcacggg gtttgaagat
     2881 catcatcgag gacctcatgc tcgacgttat gtaccagatc ccctcggacg aggacatcga
     2941 ggagtgcgtc atcacgaagg acgtcgtcaa gcaccacacg aaccccatca cgattctgcg
     3001 caaggcgggg tagaacctct tcctccccgc ttctccgcgc cccgcgcggt cctccgcctc
     3061 cggggccagg aaggaccagt agctccatga aatccaccga tatcgccacc cggacagggg
     3121 aaaccctccc gctcgtcccg cttcgggaca tggtggtctt cccgcacatg atggcgccct
     3181 tcgtggtcgg gagagattct tcgatccggg cgctcgaggc ggcgctcgcg acctccggca
     3241 agcgcctctt cctgtcggcg cagaaggacc cgcagctgga cgaaccgaag cccgacgaca
     3301 tccaccccat gggcgtcgtc gcgacggtcg cccagtccct gaagctcccc aacggccaca
     3361 tcaaggtcat ggtcgagggc gcccgtcgcg gtcggatcgt cgggttcgaa gagcgagccg
     3421 gatacctcgc cgtttcgatc gaggccgtcc cgacgcgctc ggccgacgtc gaagagctca
     3481 aggagtacat ggggaaggtt ctcggtctct tcgagcagta cgcgaagctg tcgcaccacc
     3541 tcgcgttcga ggggctggcc gcgtctctca agatcgacga cccggaccgc ttcgccgaca
     3601 cgctgtcggc gcacctcgcc gtctcgactc cggaaaagca ggctctcctc gaggcgattt
     3661 ctccgcacga gcggctccag aagctccagg acctgctcga ggtcgagatc gagaaggtga
     3721 acatcgaccg gcggatcaac aacaaagtca agaagcagat ggagaaggcc cagaaggagt
     3781 actacctcaa cgaaaagatc aaggccatcc atcaggagct cggcagaaag gacgaccgga
     3841 ccgacgaggt cgacgatctg aagaagaagt tggcgacgtc cgggatgccg aaagaggtcc
     3901 gcgagaaagc cgagcaggag ctgcggcggc tggaggcgat gccgcccgtt tccgccgaag
     3961 caacggtctc gcgcaactac atcgagtggc tcgtgggcgt tccctggaga aaggcgtcac
     4021 gggagtcgaa ggacctctcc cgcgccgaga agatcctgaa cgaggaccac ttcggcctgg
     4081 agaaggtcaa ggagcgcatc ctcgagtttc tcgcggtgcg ccagctcgtc cagaagacga
     4141 aaggctcgat catctgcttc gtcggcccgc ccggcgtcgg caagacgtcg ctcgcgaagt
     4201 cgatcgcgcg cgcgctgaac cgcaagttcg tgcggctgtc actcggcggc gtgcgtgacg
     4261 aggcggaaat ccggggccac cggcgcacct acatcggcgc cttccccggc cagatcatcc
     4321 agatgatgaa gaaggcgggg acggtgaacc ccgtcttcct gctggacgag gtcgacaaga
     4381 cgtcgtcgga cttccgggga gacccggcgt cggcgctgct cgaggtcctc gacccggagc
     4441 agaaccacac gttcgtcgat cactacctcg acgtcgaata cgacctctcc aaagtgatgt
     4501 tcatcgcgac ggcgaacgtc gcggacccga tccccccggc gttgaaggac cggatggagc
     4561 tcatcccgct ctccggctat acgcccaacg agaagctggc gatcgcgcag aagtatctgg
     4621 tttcgaagca gcgtgaggcg aacgggctga agccggacca gatcgacgtc tccgacgaag
     4681 cgatcctctc gctgatctcg aactacacgc gcgaagcggg cgtccgcaac ctggagcgcg
     4741 agatcgcgaa cgtctgccgc aaggtcgccc ggaagatcgt gcagaaaaag gtcgagccgc
     4801 cagtccaggt gaccggcgac aacctccgcg acttcctcgg caagcacaag ttccggaacc
     4861 gccggaaggg cgaggtctcg gaggtcggta ttgcgacggg gctcgcgtgg accgaggcgg
     4921 gcggcgagat cctgacgacc gaaacgagcc ttctgaaggg gaagggcaac ctgacgctga
     4981 ccggatcgct cggcgacgtc atgcaggagt cggcgcgcgc ggcggtctcg tacgtgcgca
     5041 gccgcgccga gatgtacaac gcggatccta acttccacga gaagtac
//
LOCUS       DASWAV010000541         1735 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U2665
            k141_311118, whole genome shotgun sequence.
ACCESSION   DASWAV010000541 DASWAV010000000
VERSION     DASWAV010000541.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960316
            Sequence Read Archive: SRR10260007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 1735)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1735)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:47:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,929
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1735
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_311118"
                     /isolate="SMAG_U2665"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.36444 N 132.31889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..516)
                     /locus_tag="VF958_11340"
     CDS             complement(<1..516)
                     /locus_tag="VF958_11340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9149744.1"
                     /translation="MMTDLLSLLLAGALQASTPPARTAAGPTEPALLGLAVNLPDSAS
                     PADRQKALDVVRGAGVSVFAFEVSWPEAERQPGRYDVEQVTQAARLLRQSGAVLHLDL
                     PLVNGRARVTPPALDRLAFDDPRLSLALGRLLDALGPALLDFSTLSLGNEADSYFADK
                     PSELAAFRRLFD"
     gene            560..1072
                     /locus_tag="VF958_11345"
     CDS             560..1072
                     /locus_tag="VF958_11345"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF025002.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HEX9149745.1"
                     /translation="MKTTRSVAALAAALGSLAALGCQTASSPPSTATTTAAAPTPTPY
                     PAAPAVAHAEHIKLQHILIAFAGKVAGKNITRSQDEARALANQVFERAKKGEDFDSLV
                     RTYTDDRAPGIYSLSNSGVAPAAGEFSRDRMVPAFGNVGFSLAPGEIGMAEYDPRTSP
                     YGWHIIKRLE"
     gene            1077..>1735
                     /locus_tag="VF958_11350"
     CDS             1077..>1735
                     /locus_tag="VF958_11350"
                     /inference="COORDINATES: protein motif:HMM:NF013123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DPP IV N-terminal domain-containing protein"
                     /protein_id="HEX9149746.1"
                     /translation="MRFRAPSIAATAAAALLLLPTPGEAQRKKLSVEDLTVEPPIAGR
                     PVTGVTWIGHGDRFSYLVRKGSGETAVSELWVEETATGQKKMIVSTSGLAFPEEPEPA
                     KGPPPGEKKAERPRTASLDGYAWSPDGRRVLVNGSDDLWLYDVVAGRLERLTHTAGRE
                     ELSSFSPDGSRVAFVRANDLYAVELDSGRETRLTRDGGALVYNGKVDWVYEEELAGRD
                     AR"
BASE COUNT          303 a          588 c          606 g          238 t
ORIGIN      
        1 gtcgaagagt cggcgaaaag ccgcgagctc ggagggcttg tcggcgaagt aggagtcggc
       61 ttcgttgccg agagagagcg tcgagaaatc gagcagcgcc gggcccagcg cgtcgagcag
      121 gcgcccgagc gcgagtgaca ggcgcggatc gtcgaacgca agccggtcga gcgcgggcgg
      181 agtcacgcgc gcacggccgt tcacgagagg caggtcgagg tggaggaccg ctccggactg
      241 ccgcagaagt cgtgcggcct gcgtcacctg ctcgacgtcg taccggccgg gctgacgctc
      301 cgcctccggc caggagacct cgaaggcgaa cacggagacg ccggcgcccc ggacgacatc
      361 gagcgccttc tgccgatcgg caggagaagc cgagtccggc aggttgaccg cgaggccgag
      421 aagcgccggc tctgtcgggc cggcagccgt ccgagcggga ggcgtcgacg cttgaagggc
      481 tcccgccagg agaagcgaca agaggtcggt catcaccgga ctattgtgca cgccgaatag
      541 aatccggggc ggaggtgaca tgaaaacgac gcgctcggtc gcggcgctcg ccgccgccct
      601 cggatctctc gctgcgctcg gctgccagac cgcatcctcc cctccgtcga ccgccacgac
      661 cacggcggca gctccgaccc ccacgcccta cccggccgca ccggccgtcg ctcacgcgga
      721 gcacatcaag ctccagcaca ttctgatcgc gtttgccggc aaggtcgccg gaaagaacat
      781 cacgcggagc caggacgaag cgcgcgcgct cgcgaaccag gttttcgagc gggcaaagaa
      841 gggcgaggac ttcgactcgc tcgtccggac ctacaccgac gaccgtgcgc ccggcatcta
      901 ctctctctcg aactcgggcg tcgcccccgc cgccggcgag ttctcgcgcg atcggatggt
      961 ccctgcgttc ggcaacgtcg gcttctcgct cgcgcccgga gagatcggga tggccgagta
     1021 cgaccctcgg acaagtccct acggctggca catcatcaag cggctcgagt agaacggtga
     1081 gattccgcgc gccgtcgatc gccgcgaccg ccgcggcggc gctcctcctt cttccgacgc
     1141 cgggcgaagc gcagagaaag aaactctcgg tcgaagacct gacggtggag cctccgatcg
     1201 cggggcgccc cgtcaccggc gtgacctgga tcggccatgg cgatcgcttc tcctatctcg
     1261 tgcgcaaggg ctccggcgag accgcggtat cggagctctg ggtcgaggag actgcgaccg
     1321 gccagaagaa gatgatcgtc tcgacatccg gcctcgcgtt cccggaggag ccggagccgg
     1381 cgaagggacc gccacccggg gagaaaaagg cggagcggcc acgcacggcg tcgctcgatg
     1441 gatacgcctg gtcccccgac gggcggcgcg tgctcgtgaa cggcagcgac gatctctggc
     1501 tttacgacgt ggttgccggc cggctcgagc gtctcaccca tacagccggc cgcgaggagc
     1561 tttcctcttt ctcgccggat ggaagccggg tcgcattcgt tcgggccaac gacctgtacg
     1621 cggtcgagct cgacagcggc cgcgagacga ggttgacccg cgatggcggc gccctcgtct
     1681 ataacggaaa ggtcgactgg gtgtacgagg aggagctcgc cggccgggac gctcg
//
LOCUS       DASWAV010000542        10221 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U2665
            k141_431613, whole genome shotgun sequence.
ACCESSION   DASWAV010000542 DASWAV010000000
VERSION     DASWAV010000542.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960316
            Sequence Read Archive: SRR10260007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 10221)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10221)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:47:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,929
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10221
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_431613"
                     /isolate="SMAG_U2665"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.36444 N 132.31889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..609)
                     /locus_tag="VF958_11355"
     CDS             complement(<1..609)
                     /locus_tag="VF958_11355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015919901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX9149747.1"
                     /translation="MSQSAAIPEHVLEVRDLRVSYGAIEALKGISLRLDAGEIVTLIG
                     ANGAGKSTTLRSIMGLVPPSGGEILFHGNSTAEVPTHKLVRDGLILVPEGRAIFANLS
                     VKENLEMGAYSHRDGKATDRGFEKVYSIFPRLKEREKQTGGTLSGGEQQMLAIGRALM
                     SRPRVLLLDEPSLGLAPILVHTIFEAIEAIHQEGTPVLLVEQN"
     gene            complement(606..1367)
                     /locus_tag="VF958_11360"
     CDS             complement(606..1367)
                     /locus_tag="VF958_11360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019542598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX9149748.1"
                     /translation="MALLELSRVTMRFGGLVAVNELDLSLEAGQLYGLIGPNGAGKTT
                     VFNVITGVYRPTSGQIRFKEQSIVGERSGKITRRGVARTFQNIRLFGDMTVLENVKVA
                     SHCRQTADLVSAVFRTPAHHNEEERVDRQSRELLEIFHLEAFANEKARNLPYGSQRRL
                     EIARAMATLPRLLLLDEPAAGMNPQEARQLMDLIRWVRDRFEMAILLVEHNMKVVMGV
                     CETVHVLDYGATIAVGPPSEIQSNPKVIEAYLGGA"
     gene            complement(1377..2390)
                     /locus_tag="VF958_11365"
     CDS             complement(1377..2390)
                     /locus_tag="VF958_11365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015352800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEX9149749.1"
                     /translation="MKSLVKSIALVGALLLLNALFTGSLVPGFSISPYFLQILCLAGI
                     NIVLAVSLNLINGFTGQFSIGHAGFMAIGAYVSAFLSITFGDRIRAAFSFAPVSVQNS
                     GLLLVVLAAGALAAAIAGFLVGVPSLRLRGDYLAIVTLGFGEIIRVFIVNIDAIGGAR
                     GLGGMPRLANFFWIYLFAAATVAAVTRIVNSSFGRTLIAIREDEIAAEAMGVNTTRHK
                     VLSFVVSSAMAGVAGGLFAHYLMYLHPNSFTFIKSFEIIIMIVIGGLGSITGSVLGAV
                     LYIVLTEGLREFAQYRMVLFSLLLIVIMIVRPGGILGHRELWSFFRRRRAVEAPMPPA
                     VSG"
     gene            complement(2387..3283)
                     /locus_tag="VF958_11370"
     CDS             complement(2387..3283)
                     /locus_tag="VF958_11370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015358690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEX9149750.1"
                     /translation="MTTFFQQLVNGISLGSIYALIALGYTMVYGVLRLINFAHGDVYM
                     VGAYVGYYLSRKLKGAEPSLFGALVVMLGAMVACAILGVIIERFAYRPVRRAARLTLL
                     ITAIGVSLFIENGAQLVFGPDPKFFPSLAPRADFIVGGVRLTSEQLTVIALSFLLMVL
                     LRFFILKTRTGKAMRAVSFNLDAAKLMGISTDRIIAITFGLGSALAAAAGVLIGMQIP
                     KIDPLMGINYGLKAFVAAVLGGIGNVPGAVLGGLLIGTSEVMVVGYLSSTYRDAIAFG
                     ILILVLLLRPQGILGRAQKEKV"
     gene            complement(3340..4476)
                     /locus_tag="VF958_11375"
     CDS             complement(3340..4476)
                     /locus_tag="VF958_11375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007416612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX9149751.1"
                     /translation="MKSRVLLAVLSAALLVPACRRGGGDEILVGEYGSLTGTTATFGQ
                     STNNAIQMAFEQINAAGGVLGKKVRVVVEDDQSKPEEAATAVTKLINQNHVVAMLGEV
                     SSSRSLAAAPICQANGVPMISPSSTNPRVTQVGDYIFRVCFIDPFQAEVGARIAWEIL
                     HLKKVAILSDVRNDYSVGLQTFFRQTFKGFGGQIVAEQSYSEGDSDFRAQLTQIKSAN
                     PEGIYVPGYYTEVGTIARQARELGITVPLIGGDGWDSPRLWEIGGEALNGCHFSNHYS
                     VDDPSPAVQKFVADYKAKYNQVPDALAALGYDAARILADAMTRAGTTKGDKVRDALSA
                     TKDFAGVTGTITINKERNAVKPAVVLKIENGKFVYVTTVKPTGT"
     gene            4774..6057
                     /gene="pcnB"
                     /locus_tag="VF958_11380"
     CDS             4774..6057
                     /gene="pcnB"
                     /locus_tag="VF958_11380"
                     /EC_number="2.7.7.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR01942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:1990817 - poly(A) RNA polymerase activity
                     [Evidence IEA];
                     GO_process: GO:0006378 - mRNA polyadenylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polynucleotide adenylyltransferase PcnB"
                     /protein_id="HEX9149752.1"
                     /translation="MRPRSDHPISRGQIDPDALKVLYRLKNAGYKAYLVGGSVRDLLL
                     GRTPKDFDIGTDAHPQVVRRLFRNCRLIGRRFRLAHILFPEGKVIEVATFRRRPEPLD
                     PEAPEILMTSDNVFGTAREDALRRDFTINGLFYNVADFSVIDYVGGLDDLDAGLIRTI
                     GDPDIRFREDPVRMMRGVEFASRLGFAITPDSYEAILDHRKEIAKSAPPRVTEELSQS
                     LRGGHALPTFLLMREVGLLDVLLPELAGVLREIDPEHPHGAGHLFWALLDVLDAERRR
                     GRVFDDAALFSLFFLPVVRSRLREMRLSGDPDPGRLVTVIDEAVTPVAIRMTLPNAVS
                     HRIKQALATVGRLSHRPDNRVATRRIAFREAFPVALDLFELNAMATGRGDDLVQEWRQ
                     VESRARGARERFAETAAPPRARRRRRRGGKRRAIP"
     gene            6115..7023
                     /gene="speB"
                     /locus_tag="VF958_11385"
     CDS             6115..7023
                     /gene="speB"
                     /locus_tag="VF958_11385"
                     /EC_number="3.5.3.11"
                     /inference="COORDINATES: protein motif:HMM:TIGR01230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008783 - agmatinase activity [Evidence
                     IEA];
                     GO_process: GO:0008295 - spermidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="agmatinase"
                     /protein_id="HEX9149753.1"
                     /translation="MFLSTNFLGIPEAENTLEGSRVVVLPVPYERTVSYGVGTRNGPA
                     AILEASRYVELYDDELDEEPSRLGIHTLPPWLPDRMEPEAAVRSLEGIVSELLAQPRF
                     VLTLGGEHAIAAGPIKAHLPRYPKLSILHFDAHGDLRDEYEGDRYSHACAARRFVELG
                     LPTVHVGIRSISREELDYAREKNLLIISNRRLRRFDSWIPEALERLTGDVYVTFDVDF
                     FDGSLVPGTGTPEPGGGTWEDALEIFRRVAAKRRIVGADVVEHAPLAGNRAPDFLVAK
                     LCYKLLGYALFPEKVRSLAAAEAKSF"
     gene            7097..7708
                     /locus_tag="VF958_11390"
     CDS             7097..7708
                     /locus_tag="VF958_11390"
                     /inference="COORDINATES: protein motif:HMM:NF019265.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEX9149754.1"
                     /translation="MSAEEKALVDRCREGDDDSWRDFVDRYGQKVYSVAYHFTLNRED
                     AEELSQEIFLKTFENLHRYDGSFPLVAWLLSLARNLCIDRYRRRKREKSFRFVSDEAV
                     MPLLKSDDDPAAEALKKERTKLLFWALAEIPEDLAEILVLRDLDGLAYDEIGKALDLP
                     DGTVKSRLFRARAEVARRIRERHERPSGSSAFQTLALAAGGIL"
     gene            7705..8505
                     /locus_tag="VF958_11395"
     CDS             7705..8505
                     /locus_tag="VF958_11395"
                     /inference="COORDINATES: protein motif:HMM:NF024881.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-HC2 domain-containing protein"
                     /protein_id="HEX9149755.1"
                     /translation="MSCGELERMFLAGASPEEARRHSSECATCRSLGADVDAAQGMVA
                     GLASPALPASLRGALLAIPRKTVSCEGAERLLPLAIENEISDDEERRLKSHLSRCTGC
                     SEAAATLAAARDLAAPVPPPWLAARLAANRPAREKGFWRFLLAPKAAIALAYAAAVIV
                     MLLGFNPADIARRVSASQLGENTRAAVTVAESSFTDRVGAVQERISRTFQVWKGRASG
                     YGRAALTNAVALIWKSSGSKERPAERPRNRDGRGAFREKDTTIMTWRA"
     gene            8528..9145
                     /locus_tag="VF958_11400"
     CDS             8528..9145
                     /locus_tag="VF958_11400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9149756.1"
                     /translation="MDDNRFEAPAPPPAPPPVGTIPPPPMPATTTATAPVVVLEKKSP
                     GLAGVLSMFPGLGHLYLGLYQRAFAFAGVFCLLIFFNSHGRMAPLSGPATAFVWFFAI
                     IDAVRQAKAINSGLIDTGGLAPSERVALSRPRESMAGLTWGVILVGLGALWLLDRYVD
                     LDAFWAAVEDWLAPVAFILLGVVLIVTYVAKKRRHNASETWPPRT"
     gene            complement(9221..>10221)
                     /locus_tag="VF958_11405"
     CDS             complement(9221..>10221)
                     /locus_tag="VF958_11405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018972952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="peptidase dimerization domain-containing
                     protein"
                     /protein_id="HEX9149757.1"
                     /translation="RKLPVNLVLVADGEEEIGSPHFPQIVRRPEVQAALSKTTGVFMP
                     SASQGADGVVQVSLGAKGVIEVELTSSGEKWGRGPRLDVHSSNKARIDSPAWHLVQAL
                     HTLVSPSGEPAIPGFADRARPISAAEKTMVAEGERRFDEAVMKKQLGTERWINDVGFV
                     ESLERLVSQPTVNIEGLVGGYTGPGGKTILPHRAVAKLDLRLVPDMKASEALAALKNH
                     LAKQGFGDLDVNMTGGYDPTSTSANAPLIRAQVSVLRRDGIDPLMWPRNAGSWPGYVF
                     TGEPLSLAAGHFGLGHGSGAHAPDEYYVIESANPKIQGFDGAVRSFVEYLAELATI"
BASE COUNT         1734 a         3426 c         3375 g         1686 t
ORIGIN      
        1 gttctgctcg acgaggagca ccggcgtgcc ctcctggtgg atcgcctcga tcgcctcgaa
       61 gatcgtgtgg acgaggatcg gcgcgaggcc gagcgacggc tcatccaaga gcagcacccg
      121 gggccgcgac atgagcgcgc gtccgatcgc gagcatctgc tgctcgccgc cggaaagagt
      181 cccgccggtc tgcttctcgc gctccttcaa cctcggaaag atcgaataga ccttctcgaa
      241 gcctctatcg gtcgccttcc cgtcgcgatg ggagtacgcc cccatctcga ggttctcctt
      301 cacggagagg ttcgcgaaaa tcgcccgacc ctccggcacg aggatgaggc cgtcccgcac
      361 gagcttgtgg gtcgggacct ccgcggtgga gtttccatgg aacaggatct cgcctcccga
      421 cggtggcacg aggcccatga tcgagcgcag cgtggtggac ttgccggcac cgtttgctcc
      481 gatcagagtc acgatctctc ccgcgtcgag gcgaagcgag atgcccttca gcgcctcgat
      541 cgcgccgtac gagacgcgca gatcgcggac ctcgaggacg tgctcgggga ttgcagcgga
      601 ttggctcacg cgccccccag gtacgcttcg atgaccttcg ggttgctctg gatctcggag
      661 ggaggaccga cggcgatcgt cgcgccgtag tcgagaacgt gcaccgtctc gcagacgccc
      721 atgacgactt tcatgttgtg ctcgacgagg aggatcgcca tctcgaagcg gtctcgcacc
      781 cagcggatca ggtccatgag ctgccgcgcc tcctgggggt tcatgcccgc agcgggctcg
      841 tccaggagga gcagccgcgg caacgtcgcc atcgcgcgtg cgatctcgag acgtcgctgg
      901 ctgccgtagg gcaggttcct cgccttctcg ttggcgaacg cttcgaggtg aaagatctcg
      961 agcagctcgc ggctctggcg gtcgacgcgc tcctcctcat tgtggtgggc cggcgtccgg
     1021 aagaccgcag agacgaggtc cgccgtctgg cggcagtgcg acgcgacctt cacgttctcg
     1081 agcacggtca tgtcgccgaa gagccggatg ttctggaacg tccgcgcgac gccgcggcgc
     1141 gtgatcttcc cggagcgctc gcccacgatc gattgttctt tgaagcggat ctggccggac
     1201 gtgggacggt agacgcccgt gatgacgttg aagaccgtgg tcttgcccgc tccgttcggg
     1261 ccgatcagcc cgtagagctg gccggcctcg agcgagaggt cgagctcgtt gacggcgacg
     1321 agtccgccga agcgcatcgt gacgcgggag agctcgagga gggccactgc gggctcttat
     1381 cccgagacgg cggggggcat cggcgcctcc acggcccgcc ggcgccggaa gaacgaccag
     1441 agctcccggt gtccgagaat tccccccggc cgcacgatca tgatcacgat caggagcagc
     1501 gagaaaagga ccatccggta ctgggcgaac tctcgaagcc cttcggtgag gacgatgtag
     1561 aggacggcgc ccaggaccga tccggtgatc gagccgaggc ctccgatcac gatcatgatg
     1621 atgatctcga acgacttgat gaacgtaaac gagttcgggt gcaggtacat caggtaatgg
     1681 gcgaagagtc cgcccgcgac gccggccatc gcggacgaca ccacgaatga aaggactttg
     1741 tgccgggtcg tgttgacccc catcgcctcg gccgcaatct cgtcctcccg gattgcgatc
     1801 agggtccgtc cgaacgagga gttgacgatc cgggtgacgg ccgcgaccgt cgctgccgcg
     1861 aagaggtaga tccagaagaa attcgcgagc cgcggcatgc cgcccagtcc acgggcgccg
     1921 ccgatggcgt cgatgttgac gatgaacacg cggatgatct ctccgaaacc cagggtgacg
     1981 atcgcgaggt agtcgccgcg aaggcggaga gacggaacgc cgacgagaaa gccggcgatt
     2041 gcggcggcga gcgccccggc ggccagcacg acgagcagga ggccgctgtt ctggacggac
     2101 accggcgcga acgagaacgc cgcgcgaatc cggtcgccga acgtgatgga aaggaacgcc
     2161 gagacatacg cgccgatcgc catgaagccc gcatgcccga tcgagaactg tcccgtgaag
     2221 ccgttgatga ggttcaggct gaccgcgagg acgatgttga ttccggcgag acacaggatc
     2281 tgcagaaagt aaggggatat cgagaacccc ggcacgagac ttccggtgaa gagcgcgttc
     2341 agcagcagaa gcgcgccaac gagcgcgatg ctcttgacga gcgacttcaa accttctcct
     2401 tttgcgcgcg cccgaggatc ccctgcgggc gcaggaggag cacgaggatc aatatcccga
     2461 acgcgatcgc gtcccggtag gtcgaggaga ggtacccgac gaccatcacc tccgaggtcc
     2521 cgatcaggag accgccgagc acggctcccg gaacgtttcc gatgcccccc aggaccgccg
     2581 ccacgaacgc cttgaggccg tagttgatgc ccatgagggg atcgatcttg ggaatctgca
     2641 tcccgatgag gacgccggcc gcggccgcga gcgcggatcc gagcccgaac gtgatcgcga
     2701 tgatccggtc ggtcgagatc cccatgagct ttgcggcgtc caggttgaac gagaccgccc
     2761 gcatcgcctt gcctgtgcgc gttttcagga tgaagaaccg cagcagaacc atgagcagga
     2821 acgagagggc gatgacggtg agctgctcgc tcgtcagccg gacgccgccg acgatgaaat
     2881 ccgcgcgcgg cgccagcgac gggaagaact tcgggtccgg gccgaaaacg agctgggcgc
     2941 cgttctcgat gaagagcgag acgccgatcg ccgtgatgag gagtgtcaaa cgggccgcgc
     3001 gccgcaccgg acgataggcg aagcgctcga tgatgacgcc gagaatcgcg cacgcgacca
     3061 tcgcgccgag catcacgacg agcgctccga agagggaggg ctcggccccc ttgagcttcc
     3121 gcgaaaggta gtagcccacg tacgcgccca ccatgtacac gtcgccgtgc gcgaagttga
     3181 tgaggcgcag cacaccgtag accatggtgt aaccgagagc gatcagggcg tagatggatc
     3241 cgagagagat tccgttgacg agctgttgga aaaaagtcgt catggaagcc gtcagctatc
     3301 agctctcagc tgtcagctcc ggaagctggc gcgttccggc taggtcccgg tcggcttgac
     3361 cgtcgtcacg tagacgaact tcccgttctc gatcttcagg acgaccgccg gtttgacggc
     3421 gttgcgctcc ttgttgatgg tgatcgtgcc ggtaacgccc gcaaagtcct tcgttgccga
     3481 aagggcgtcg cggaccttgt cccccttggt cgtgccggct cgcgtcatcg cgtccgcgag
     3541 gatccgcgcg gcgtcgtaac cgagcgcagc gagcgcgtcc ggaacctggt tgtacttggc
     3601 cttgtagtcg gcgacgaatt tctgcaccgc cggcgaagga tcgtcgacgg agtagtggtt
     3661 cgagaagtgg cacccgttca acgcttcccc gccgatctcc cacagccgcg gcgagtccca
     3721 gccgtcgccg ccgatcagcg ggacggtgat gccgagctcg cgggcctggc gcgcgatcgt
     3781 gccgacctcg gtgtagtagc cgggcacgta gatgccctcc gggttggccg acttgatctg
     3841 ggtgagctgg gcgcggaagt ccgagtctcc ttcgctgtac gactgctcgg cgacgatctg
     3901 gccgccgaaa cccttgaacg tctgtcggaa gaacgtctgg aggccgacgg agtagtcgtt
     3961 gcgcacgtcg gagaggatcg cgaccttctt caagtgaagg atctcccacg cgatccgcgc
     4021 gccgacctcg gcctggaagg gatcgatgaa gcacacgcgg aagatgtagt cgccgacctg
     4081 cgtcacgcgc gggttcgtcg acgacggaga gatcatcgga acgccgttcg cctgacagat
     4141 cggagctgcc gcgagcgagc gcgaggacga aacctccccg agcatcgcga cgacatggtt
     4201 ctggttgatg agtttcgtca ccgccgtcgc cgcctcttcc ggcttcgact ggtcatcctc
     4261 gacgaccact cgcaccttct tgccgagcac cccgccggcg gcattgatct gctcgaacgc
     4321 catctggatg gcgttgttcg tcgactggcc gaaggtcgcc gtggtaccgg tcaaggagcc
     4381 gtactcgccg acgaggatct cgtcgccacc ccctcggcgg caggccggca ccagaagggc
     4441 ggctgagagg accgccagaa gcacgcgcga tttcattgtc gaaagcctcc tcgtcgggga
     4501 tgctcgaaaa agcgtgcgta ttctttccca gaggcgcgaa gaaagtcaaa acctcaattc
     4561 gatcagggag ttgagctaga atccgtcctg gatgcggatc cgtcgccggg cgggcgatct
     4621 ctttcgtccc cggtcatccg cagccgccga tggggcgcgg ggccgatgag gcccgcgttc
     4681 ttggagagct gaccgctgga tcccgaaaca accgagattc gatccgccgc gccggaagcg
     4741 ggcgccgacg tctccgagca ggtccttccg cgaatccgtc cacgcagtga tcatccgatc
     4801 tcgcgcggtc agatcgatcc ggatgcgctg aaagtcctct accggctgaa gaacgccggc
     4861 tacaaggctt acctggtcgg aggcagcgtc cgcgatctcc tactcggccg cacgccgaag
     4921 gacttcgaca tcgggacgga cgctcatccg caggttgtgc ggcgcctctt ccgcaattgc
     4981 cggttgatcg gccggcggtt ccgtctcgcg cacatcctgt ttcccgaggg aaaggtcatc
     5041 gaggtggcga cgttccgccg ccgtcccgag ccgctcgacc ccgaggctcc ggagatcctg
     5101 atgacgtcgg acaacgtctt cgggaccgcg cgcgaggacg cgctgcgacg cgacttcacg
     5161 atcaacgggc tcttctacaa cgtcgcggac ttctcggtga tcgactacgt cggaggcctc
     5221 gacgacctcg acgcgggcct gatccggacc atcggggatc cggacatccg cttccgcgaa
     5281 gacccggtcc ggatgatgcg cggagtcgag ttcgcgagcc gcctcggttt cgcgatcact
     5341 cccgattcgt acgaagcgat cctcgatcac cgcaaggaga tcgcgaagtc cgcgcctccc
     5401 cgcgtcaccg aagagctctc gcagtcgctc cgcggcgggc atgcgcttcc gacgtttctc
     5461 ctgatgaggg aggtcggtct cttggacgtc ctgttgcccg agctcgccgg ggtgctccgc
     5521 gagatcgatc ccgagcaccc gcacggcgcc ggccacctct tctgggcgct gctcgacgtg
     5581 ctcgacgccg agcggcggcg cggccgagtc ttcgacgacg ccgccctgtt ctcgctcttc
     5641 ttcctgcccg tcgtccgatc gcgcctccgc gagatgcgtc tctcgggcga cccggacccg
     5701 gggcgcctcg tcaccgtgat cgacgaagcc gtcacaccgg tcgcgatccg gatgaccttg
     5761 ccgaacgccg tgtcccaccg catcaagcag gctctcgcga ccgtcggcag gctgtcgcac
     5821 cgtcccgaca accgcgtcgc gacgcggcgg atcgcctttc gggaagcgtt cccggtggcg
     5881 ctcgacctct tcgagctgaa cgcgatggcg acgggacgcg gagacgatct cgtgcaggag
     5941 tggcgccagg tggagtcgag agcgcgaggg gcgcgggaga ggttcgccga gaccgcggca
     6001 ccgccgcgtg cgcggcgtcg gagacggagg ggcggaaaga ggcgcgcgat cccctgacga
     6061 tccgcgccgg tgcgaagcgc atcgaagcga agggcgaaag gtaagatccg atccatgttt
     6121 ctgtccacga acttcctcgg catccccgaa gcggagaaca cgctcgaggg cagccgcgtc
     6181 gtcgtcctgc cggtccccta cgaacgaacc gtctcctacg gcgtcggaac ccgaaacggc
     6241 ccggcagcga tcctcgaagc gtcgcgatac gtggagctct acgacgacga gctcgacgag
     6301 gagccgtcgc ggctcgggat ccacacgctc ccgccgtggc tgcccgaccg gatggagccg
     6361 gaagcggccg ttcgctcgct cgagggcatc gtctccgagc tgctcgcgca gccgcgcttc
     6421 gttctcacgc tcggaggcga gcacgcgatc gccgcgggtc cgatcaaggc ccaccttccc
     6481 cggtacccga agctctcgat cctgcacttc gacgcacatg gagacctgcg cgacgagtac
     6541 gagggggaca ggtactccca tgcctgtgcg gcgcgccgtt tcgtcgagct cgggttaccg
     6601 acggtgcacg tcggcatccg gtcgatctcg cgggaggagc tcgattacgc gcgggaaaag
     6661 aatctcctga tcatctcgaa ccggcgcctg cgccggttcg actcctggat cccggaggcg
     6721 ctcgagcgcc tgacaggcga cgtttacgtg actttcgacg tcgacttctt cgacggttcg
     6781 ctcgtcccgg gcacgggaac ccccgagccg ggagggggca cctgggaaga cgcgctcgag
     6841 atcttccggc gcgtcgcggc gaagcgccgg atcgtgggcg ccgacgtcgt cgagcacgcc
     6901 ccgctcgccg gcaaccgcgc gcccgatttc ctcgtggcca agctctgcta caagctgctc
     6961 gggtacgcgc tctttccgga aaaagtccgt tctctcgcgg cggcggaagc caagtccttt
     7021 tgaatcagcg tcacaggccg gacggacctc cgaactttcc gcccgaacct gcgtttctta
     7081 gggcgagagg aaacgtttga gcgccgaaga aaaagccctg gtcgaccgct gccgcgaagg
     7141 ggacgacgac tcctggcgcg acttcgtcga ccggtacggc cagaaggtct actcggtggc
     7201 ctatcacttc acgctcaacc gggaggacgc cgaagagctc tcgcaggaga tcttcctcaa
     7261 gaccttcgaa aacctccacc gctacgacgg aagcttcccg ctcgtcgcct ggctcctttc
     7321 gctcgcgcgc aacctctgca tcgaccggta ccgccggcgc aagcgcgaga agtcgttccg
     7381 cttcgtcagc gacgaggcgg tcatgcctct cctgaagtcc gacgacgatc ccgccgccga
     7441 ggctctgaag aaggagcgca cgaagctcct cttctgggcg ctcgccgaga tccccgagga
     7501 cctcgcggag atcctcgtcc tgcgcgacct ggacggcctc gcctacgacg agatcggaaa
     7561 agccctcgat ctgccggacg gcaccgtcaa gtcgcgcctc tttcgcgcgc gggccgaggt
     7621 cgcgcggcgg atccgcgagc ggcacgagag gccgagcggt tcgagcgctt tccagacgct
     7681 cgcgctcgcc gccggaggaa ttttgtgagc tgcggcgagc tcgaacggat gttcctcgcc
     7741 ggcgcgtcgc cggaagaagc ccgacggcat tcgagcgagt gcgcgacgtg ccgctccctc
     7801 ggagcggacg tggacgccgc gcaggggatg gtcgccggac tcgcgagccc cgcccttccg
     7861 gcctctctgc ggggggctct tctcgcgatc ccccgcaaga cggtctcctg cgaaggcgcc
     7921 gagcggcttc tcccgctcgc gatcgagaac gagatctcgg acgacgagga acggcgcctc
     7981 aagagccacc tgtcgcgctg cacggggtgc tccgaggccg cggcgaccct cgcggcagcg
     8041 cgcgatctgg cggctccggt cccgccgcct tggctcgcgg ctcgtctcgc ggccaaccgg
     8101 ccggcccgcg agaagggctt ctggcggttc ctgctcgcgc cgaaggcggc gatcgctctc
     8161 gcgtacgccg ccgcggtcat cgtgatgctc ctcgggttca atccggccga catcgcgcgc
     8221 cgcgtctcag cgagccagct cggggagaac acgcgcgcgg cagtgaccgt cgccgagagc
     8281 tcgttcaccg accgcgtggg cgctgtccag gaaaggattt cgcgcacctt ccaggtgtgg
     8341 aaggggcgcg cgagcggtta cgggcgcgcg gctctcacca acgcagtcgc gctcatctgg
     8401 aaatcatcgg gctccaagga gcgcccggcc gagcgcccac ggaaccggga cggccgcggc
     8461 gcgtttagag agaaagacac gacgatcatg acctggcggg cgtaaggccg ccgagaggag
     8521 aaacgaaatg gacgacaacc gctttgaggc tccggcgcct ccacccgccc ctcccccagt
     8581 cgggacgatc ccgccgccgc cgatgcccgc gacgacgacc gcgacggctc cggtggtcgt
     8641 gctcgagaag aagtcgccgg ggctcgccgg cgtcctctcg atgtttcccg ggcttgggca
     8701 tctgtacctg ggcctctatc agcgagcgtt cgcctttgcc ggcgtcttct gcctgctgat
     8761 cttcttcaac tcccacggcc ggatggctcc cctctcgggc ccggcgaccg ccttcgtgtg
     8821 gttcttcgca atcatcgacg ccgttcggca ggccaaagcg atcaactccg gcttgatcga
     8881 cacgggaggt ctcgccccct ccgaacgggt ggcgctctcc cgcccgcggg aatcgatggc
     8941 gggcctgacc tggggcgtca tcctcgtcgg cctcggcgcg ctctggctgc tcgaccgcta
     9001 cgtggacctc gacgcgttct gggcggccgt cgaggactgg ctcgccccgg tggcgttcat
     9061 cctcctcggg gtggtcctga tcgtgacgta cgtggcgaag aaacgccgcc acaatgcgtc
     9121 ggagacgtgg ccgccgagaa cgtaaccgcc ctcaccgcaa ccctctcccc gccggcgggg
     9181 agagggagag ctcacgaggc gcagcaacgc gccttttctt ttagatggtc gcgagctcgg
     9241 ccagatattc cacgaaggac ctgaccgctc cgtcgaatcc ctggatcttc gggttcgccg
     9301 actcgatcac gtaatactcg tcgggcgcgt gcgcgccgct cccgtggccg aggccgaagt
     9361 ggccggctgc gagcgagagc ggctcgcccg tgaaaacgta tccgggccac gagccggcgt
     9421 tgcggggcca catgaggggg tcgatcccgt cgcgccggag caccgagacc tgcgcgcgga
     9481 tcaaaggcgc gttcgcggag gtgctcgtcg gatcgtagcc gccggtcatg ttgacgtcga
     9541 gatctccgaa gccctgcttc gcgaggtggt tcttcagcgc cgcgagcgct tccgacgctt
     9601 tcatgtccgg cacgagccgc aggtcgagct tcgcgacggc ccggtgcggc aggatcgtct
     9661 taccgcccgg cccggtatat ccgccgacca gaccctcgat gttgacggtc ggctgcgaga
     9721 ccagccgttc gagcgactcc acgaagccga cgtcgttgat ccaacgctcg gttccgagct
     9781 gtttcttcat caccgcctcg tcgaatcggc gctctccctc ggcgaccatc gtcttctccg
     9841 ccgcggagat cggacgcgcg cgatcggcga atcccggtat cgccggctcg ccgctcggcg
     9901 acaccagcgt gtggagcgcc tggacgaggt gccaggccgg actgtcgatt cgtgccttgt
     9961 tgctcgagtg gacgtccagg cgcgggcccc ggccccactt ctctccgctc gacgtcagtt
    10021 cgacttcgat gacacctttt gcaccgagtg acacctgcac gacgccgtca gcgccctgcg
    10081 acgcggacgg catgaagact cccgtcgttt tcgagagcgc cgcctgcacc tcgggccgcc
    10141 ggacgatctg ggggaagtgc ggcgagccga tctcctcctc accgtccgcg acgaggacga
    10201 ggttcaccgg cagcttccga c
//
LOCUS       DASWAV010000543         3417 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U2665
            k141_30670, whole genome shotgun sequence.
ACCESSION   DASWAV010000543 DASWAV010000000
VERSION     DASWAV010000543.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960316
            Sequence Read Archive: SRR10260007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 3417)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3417)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:47:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,929
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3417
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_30670"
                     /isolate="SMAG_U2665"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.36444 N 132.31889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1196)
                     /locus_tag="VF958_11410"
     CDS             complement(<1..1196)
                     /locus_tag="VF958_11410"
                     /inference="COORDINATES: protein motif:HMM:NF024900.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HEX9149758.1"
                     /translation="MSAPAPPATSPLAAPRKETLTIVFDGQPVEVPPGINLIEAAKLA
                     GVEIPHFCFHPRLPVVGQCRMCLVEIEGIPKIQAACTTPLKDGLVVKTTTDKVRDSRA
                     ANMEFLLINHPLDCPICDQAGECKLQDNAFGYGEQRSRYDERKRQYAGFDRTMIGPHV
                     VADMTRCIQCTRCIRFCSEIAGTGELTFLDRGGRTLVWTHDGKPLANDWSACAADVCP
                     VGALTTKEFRFRRRVWDLNKTPSVCPGCNIGCNISIESRDGIVYRFLPRLNPSVNDYG
                     LCDYGRFQSESLNERDVAKAAVRDGAAVKDATVPGAIERIAAEIRKTIDAEGPSALFF
                     LGSAHLSNEENFLLRKLADHLGCGNLDVVVDRSRVRRMKSKTEWLEGDHCGANYAGAV
                     DMGVAPR"
     gene            complement(1193..2509)
                     /gene="nuoF"
                     /locus_tag="VF958_11415"
     CDS             complement(1193..2509)
                     /gene="nuoF"
                     /locus_tag="VF958_11415"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013112105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HEX9149759.1"
                     /translation="MTFEPILLARRGRERSQSIDTYVADGGYETLRKVLTGGWTPEAV
                     TEEVKKSGLRGRGGAGFPTGVKWGFVPKDTKGKPVYLLCNADESEPGTFKDRLLMEED
                     PHQMLEGQIISAYALRCRASYVYIRGEFFHGARVLQRAIEEAYEKGFLGENILGSGFS
                     HDITVHRGAGAYICGEETGLIESLEGKRGQPRIKPPFPAVVGVFGAPTIVNNVETICC
                     VTHVMKRGAEWFSKIGRNEKNTGPKLYCLSGHVNRPGVYEAPMGLQMKDLLFGDDFGR
                     GMKDGRKVKAVIPGGASAAMLTAAEIEDCPLDFDGLAAKKSMLGSAAIIVMNEDTCIV
                     DAATNLSKFFSHESCGQCTPCREGTPWLHRVLSRIEAGEGRREDIDLLERISNQMANG
                     MTICVFADAAIAPVLSSIGKFRDEYLHHVEHRQCLVGPRAPVEASA"
     gene            complement(2506..3015)
                     /gene="nuoE"
                     /locus_tag="VF958_11420"
     CDS             complement(2506..3015)
                     /gene="nuoE"
                     /locus_tag="VF958_11420"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012236857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoE"
                     /protein_id="HEX9149760.1"
                     /translation="MTSLRYSVRPAGSPADFPPAARERIEKILSRYPTKQAALLPVLW
                     VAQETFGWISREAAEEVARILELPAAHVSGVLTFYTMYNLEPVGRHLLQFCTSISCHL
                     AGAERLVEHCRNRLGIGLGETTPDGRFTMVEVECIAGCDRAPSMMVNDAYHEPMDAAK
                     LDALIGTLP"
     gene            complement(3012..3365)
                     /gene="ndhC"
                     /locus_tag="VF958_11425"
     CDS             complement(3012..3365)
                     /gene="ndhC"
                     /locus_tag="VF958_11425"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020674776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit A"
                     /protein_id="HEX9149761.1"
                     /translation="MAESYLPILILMLFAIAVAGGMILLSAVLGPRLRTRRKLDTYES
                     GMPLLDASHKRVSIQFFLIAMVFILFDVEIAFLYPWALVFRSGGMPLFVEMGVFLAVL
                     AVGYAYLWKKKAFDW"
BASE COUNT          585 a         1099 c         1145 g          588 t
ORIGIN      
        1 cgcggggcca cgcccatgtc gacggcgccg gcgtagttgg cgccgcagtg gtccccctcg
       61 agccactcgg tcttcgactt catgcggcgt acccgcgacc tgtccacgac gacgtcgagg
      121 ttcccgcagc ccaggtggtc cgcgagcttg cgcaagagaa agttctcttc gttcgagagg
      181 tgggcggacc cgaggaagaa gagcgccgaa ggtccctcgg cgtcaatcgt cttgcggatc
      241 tcggccgcga tccgttcgat cgcaccgggt accgtggcgt ccttcacggc ggcgccgtcg
      301 cgcaccgccg ccttcgcgac gtcgcgctcg ttgaggctct ccgactggaa gcgtccatag
      361 tcgcagagcc cgtagtcgtt gaccgaagga ttcagcctcg gcaggaagcg gtagacgatc
      421 ccgtcgcgcg actcgatcga aatgttgcaa ccgatgttgc agccggggca aaccgaaggc
      481 gtcttgttca ggtcccagac gcggcgtcgg aagcggaatt ccttcgtcgt cagcgcgccg
      541 accgggcaga cgtcggccgc gcaggcggac caatcgttcg cgagtggttt gccgtcgtgc
      601 gtccagacga gcgtgcggcc gccgcggtcg aggaacgtga gctctcccgt tcccgcaatc
      661 tcggagcaga agcggatgca ccgtgtgcac tggatgcagc gcgtcatgtc ggcgacgacg
      721 tgcgggccga tcatcgtccg gtcgaagccc gcgtactggc gcttgcgctc gtcataccgc
      781 gaacgctgct ccccgtaacc gaacgcgttg tcctgcaact tgcactcgcc cgcctggtcg
      841 cagatgggac agtcgagcgg gtgattgatc agcaggaact ccatgttcgc cgcgcgcgag
      901 tcgcggacct tatccgtggt cgtcttgacc acgagaccgt ccttcaacgg ggtcgtgcac
      961 gcggcctgga tcttggggat gccttcgatc tcgacgaggc acatccggca ctggccgacg
     1021 acgggcagcc gcgggtgaaa acagaagtgg gggatttcca cccccgccag cttcgccgcc
     1081 tcgatcaggt tgatgccggg agggacttcg acgggttgac cgtcgaagac gatcgtcagc
     1141 gtctccttcc tgggagcggc gagaggggag gtggccggag gcgcgggggc gctcacgcgg
     1201 acgcctcgac cggggcgcgc ggcccgacga ggcactggcg gtgctcgacg tgatggaggt
     1261 actcgtcgcg aaactttccg atcgaggaga gcacgggcgc gatggcggcg tcggcgaaga
     1321 cgcagatggt catgccgtta gccatctggt tcgagatcct ctcgagcagg tcgatgtcct
     1381 cccgacgtcc ttcacccgcc tcgatgcgcg agagaacccg gtggagccac ggcgttccct
     1441 cgcggcacgg cgtgcactgg ccgcacgact cgtgggaaaa gaacttcgag aggttcgtcg
     1501 cggcgtccac gatgcacgtg tcctcgttca tcacgatgat cgccgcggag ccgagcatcg
     1561 acttcttcgc ggcgaggccg tcgaagtcga gcggacagtc ctcgatctcg gcggcagtga
     1621 gcatcgctgc cgaggccccg cccggaatga cggctttcac cttccgcccg tccttcattc
     1681 cccggccgaa gtcgtcgccg aagaggagat ccttcatctg gaggcccatg ggagcctcgt
     1741 acacgccggg ccggttgacg tggcctgaga ggcagtagag cttggggccc gtgttcttct
     1801 cattgcggcc gatcttcgag aaccactccg cgccgcgctt catgacgtga gtgacgcagc
     1861 agatcgtctc gacattgttg acgatggtcg gcgccccgaa gaccccgacg accgcgggga
     1921 aggggggctt gatgcgaggc tggccgcgct tgccctccag gctctcgatc aggccggttt
     1981 cttcgccgca gatgtacgcg ccggcaccgc ggtgaacggt gatgtcgtgc gagaaaccgg
     2041 agcccagaat gttctcgccc agaaacccct tctcataggc ttcttcgatc gcgcgctgga
     2101 ggacgcgcgc gccgtggaag aactccccgc gaatgtagac gtaggaggcc cggcaccgga
     2161 gcgcgtaggc ggagatgatc tggccttcga gcatctggtg ggggtcttct tccatgagca
     2221 ggcggtcctt gaacgtcccc ggctccgatt cgtccgcgtt gcagagcaga tagaccggct
     2281 tccccttcgt gtccttcggt acgaagcccc acttgacccc cgtcggaaac cccgctccgc
     2341 cgcggccacg cagcccggat ttctttacct cttcggtgac cgcctccgga gtccacccgc
     2401 cggtcaggac ttttcgaagc gtctcgtacc ccccgtcggc gacgtacgtg tcgatcgact
     2461 gcgaccgctc gcgaccccgg cgtgcgagga gtatcggctc gaaagtcatg gcagggtccc
     2521 gatgagggcg tcgagcttgg ccgcgtccat cggctcgtga taggcgtcgt tgaccatcat
     2581 cgaaggtgct cggtcgcagc ccgcgatgca ctccacttcg accatggtga atcttccgtc
     2641 gggtgtcgtc tcgccgagtc cgatcccgag ccggtttcgg cagtgctcaa ccaaccgctc
     2701 cgcccctgcg aggtggcagg agatcgacgt gcagaattgc agcagatgcc gcccgaccgg
     2761 ctcgaggttg tacatcgtgt agaaagtgag aacgcccgac acgtgcgcgg cgggcagctc
     2821 caggatccgc gcgacctcct cggcagcctc ccgggagatc cacccgaacg tctcctgcgc
     2881 gacccagagc accggcagga gggcggcctg cttcgtggga tagcgggaca ggatcttctc
     2941 gatcctctcg cgggcggccg gtgggaagtc ggcgggggag ccggcaggcc tgacggagta
     3001 gcggaggctc gtcaccagtc gaaagccttc ttcttccaga ggtaggcgta gccgacggcg
     3061 aggacggcga ggaacacgcc catctcgacg aagaggggca ttccacccga ccggaaaacg
     3121 agggcccagg ggtagagaaa cgcgatctcg acgtcgaaaa ggatgaagac catcgcgatc
     3181 agaaagaact gaatcgagac acgtttgtgc gaagcgtcga gcagaggcat cccgctctcg
     3241 tacgtgtcga gcttgcgccg ggtacggagg cgtggtccga ggaccgcgga cagcaggatc
     3301 atcccaccgg cgacggcgat cgcgaagagc atcaggatga ggatcggaag gtagctttcg
     3361 gccatccgca cctcgcctcg ttcagacctc gcgcggcgag ctttcggagg gagccgc
//
LOCUS       DASWAV010000544         2748 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U2665
            k141_470919, whole genome shotgun sequence.
ACCESSION   DASWAV010000544 DASWAV010000000
VERSION     DASWAV010000544.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960316
            Sequence Read Archive: SRR10260007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2748)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2748)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:47:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,929
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2748
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_470919"
                     /isolate="SMAG_U2665"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.36444 N 132.31889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..206)
                     /locus_tag="VF958_11430"
     CDS             complement(<1..206)
                     /locus_tag="VF958_11430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9149762.1"
                     /translation="MRYRYGDYPVDLVRQLLTFQELTKLFLQLVLQTGGDVEEALRWM
                     QYLQERGMIDPSIDLEEFRRQLEK"
     gene            complement(249..710)
                     /locus_tag="VF958_11435"
     CDS             complement(249..710)
                     /locus_tag="VF958_11435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003932443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HEX9149763.1"
                     /translation="MLGELTPREIEDFLRTETTGRIGCHADGRTYVVPITYAYNSPYV
                     YCHSPEGLKLRMMRKNPVVCFEVDRLEDVGSWRSVIALARFEELGGRDALAAMDILIA
                     RFTALHPEGAEHPSYVLRASDVESSRADGRPIVLFRLLLAEKTGRFERVAD"
     gene            770..1429
                     /locus_tag="VF958_11440"
     CDS             770..1429
                     /locus_tag="VF958_11440"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HEX9149764.1"
                     /translation="MSSSADETLDRIARSKEAVFAIDSADRIVLWNKRAEELLGRPAR
                     AVLGKRCYEVLAGRDAQGNIYCYRNCPVAFQAREKQSQPVARFPLQVPTGNGGERWVE
                     MSLFAIPSYHPALSTVVHIVREGKKAPSSLEKKLEKEAVNHEPLWPMTSSEGEPVALT
                     EREQEVLRCLARGMSTAGIARKLAIAPVTVRNHIQSILQRLDVHSKLAAVVYAYRHEL
                     I"
     gene            complement(1446..1841)
                     /locus_tag="VF958_11445"
     CDS             complement(1446..1841)
                     /locus_tag="VF958_11445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019401566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEX9149765.1"
                     /translation="MRGFVNDIEDMTEHNSDFRRVLYTGKRLQLVLMAIEPGEDIGEE
                     IHPDRDQFFRIEKGKGEVSIDGKRTKVESDSAILVPAGARHNIRNTGDKPLKLYTLYG
                     PPEHADGTVHATKAVAMAAKEHFAGQTTE"
     gene            complement(2085..2489)
                     /locus_tag="VF958_11450"
     CDS             complement(2085..2489)
                     /locus_tag="VF958_11450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9149766.1"
                     /translation="METQVGTPPAVKLSRPLAIEKMRQKLRTLTDQDHCVCSVVGQLG
                     IFCRGFKALPEKDLRNRYHWIAKKRPNAPREMIEELANLYHIGRQEVTGAAICCDVET
                     REHAGCDGWNNFDNRQLEEFYLALTGEPAEIG"
BASE COUNT          471 a          871 c          886 g          520 t
ORIGIN      
        1 ctcttttcga gctggcgacg gaactcctcg aggtcgatcg aaggatcgat catgccccgt
       61 tcctggagat actgcatcca gcggagcgcc tcctcgacgt cgcctccggt ctggagcacc
      121 agctggagga acagcttcgt cagctcctga aacgtcagga gctggcggac gagatcgacg
      181 ggatagtcgc cgtaccggta gcgcatcgtc ccggcgattc tacggcggag atcgctcgcc
      241 ggccgcgctc agtcggcgac gcgctcgaac cggcccgttt tctcggcgag gaggagtcgg
      301 aagagaacga tcggacgtcc gtcggcgcga gaagactcca cgtcggaggc acgcaacacg
      361 taggagggat gctccgcgcc ctccggatgg agcgcggtga agcgggcgat caagatgtcc
      421 attgccgcca gcgcgtcccg cccgccgagc tcctcgaatc tcgcgagtgc gatcacgctc
      481 cgccaggacc cgacgtcctc gagccgatcg acctcgaagc agaccaccgg gttcttgcgc
      541 atcatgcgga gcttcagccc ttccgggctg tggcagtaaa cgtacggaga gttgtaggcg
      601 taggtgattg gcacgacata cgttcggccg tcggcgtggc atccgatccg gccggtcgtc
      661 tcggtgcgaa ggaaatcctc gatctcccgt ggcgtcagct cgcccagcat gtcgcctccc
      721 gagccgatcc tatgccgtgg ccggcagccg gcacgctaga atcgccagcg tgagctcttc
      781 tgccgacgag accctcgacc gcatcgcgag gagcaaggaa gccgtcttcg cgatcgactc
      841 ggcggaccgg atcgtgctgt ggaacaagcg cgccgaggag ctgctcggcc ggccggcgcg
      901 cgcggtcctc ggaaagcggt gctatgaggt cctcgccggc cgggatgctc agggcaacat
      961 ctactgctac cggaactgcc cggtcgcatt ccaggcacga gagaagcagt cgcagccggt
     1021 ggcccgtttt ccgctccagg ttccgacggg caacggcggc gagcgctggg tcgagatgtc
     1081 gctcttcgcg atcccctcgt atcaccccgc tctttcgacg gtcgtccaca tcgtccggga
     1141 agggaagaag gcgccttcga gtctcgagaa gaagctcgaa aaagaggccg tgaaccacga
     1201 gcccctgtgg ccgatgacct cctccgaagg agagccggtc gcgctcaccg aacgagaaca
     1261 ggaagtgttg cggtgtctcg cgcggggaat gtcgaccgcc ggaatcgccc gtaaactcgc
     1321 gatcgctccc gtcacggtgc gcaaccacat ccagtcgatc ctgcagcggc tggacgtcca
     1381 cagcaagctc gccgcggtcg tctatgcgta ccgccacgag ctgatctgac ggcatccggg
     1441 ccggactact cggtggtctg tccggcgaag tgctccttcg ccgccatggc aaccgccttg
     1501 gtggcgtgaa ccgtcccatc tgcgtgctcg ggcggcccgt acagcgtgta aagcttcagc
     1561 ggcttgtctc cggtgttccg gatgttgtgt cgagcgccgg cgggaacgag gatcgcggaa
     1621 tcgctttcga ccttcgtcct cttgccgtcg atagagacct cgcctttgcc cttctcgatc
     1681 ctgaagaact ggtcacggtc cggatggatc tcctcgccga tgtcctcgcc cggctcgatg
     1741 gccatcagca cgagctggag ccgcttgccg gtgtagagca cgcggcggaa gtctgaattg
     1801 tgctcggtca tgtcttcgat gtcgtttacg aaacctctca tgtcagtcat cccttctgat
     1861 gcggggatct gcgttcgcat caacgaggtg agagatacag gacggccgcg taaaccacta
     1921 gatttaggtg aatcagattc ccgtccggag tcagggcggc aaaggcgaac cccttgtatc
     1981 ggctttccgt tcaagcaggc ccgaccgagg ctgagaaacc ttccgggggt caccttcgtg
     2041 cggatcccac gccggatccg cgcgatcatc gtcgccgacc gcgatcagcc gatttcggcc
     2101 ggctcgcccg tcagggcgag atagaactcc tcgagctggc gattgtcgaa gttgttccag
     2161 ccgtcgcagc ctgcgtgttc gcgggtctcg acgtcgcagc agatcgccgc gccggtcact
     2221 tcctgccggc cgatatggta gaggttcgcg agttcctcga tcatctctcg cggggcgttg
     2281 gggcgcttct tcgcgatcca gtggtatcga ttgcgcaggt ccttttcggg aagggccttg
     2341 aagccccggc agaagatccc gagttgaccg accaccgagc agacgcagtg gtcctggtcc
     2401 gtcagggtgc ggagcttctg gcgcattttt tcgatcgcca gcgggcggga gagtttcacg
     2461 gcggggggcg tcccaacttg cgtttccatg gccggatcgt gctccggcgc gaggccggcc
     2521 gcctgattca aacgaatcag tgcaccggaa acagtcccgg aggcgcgaaa cccctgaaaa
     2581 gacgggcttt ctccatcgtt tccggaaccg gtatcgggta tgaccgggat catgcggccg
     2641 ggaggctcgg tctcctacga tcggtctgtc catgaggtgt gaagatcggc gcatgagact
     2701 ccgggctccg atcgttcggt tggcagtgac gtgtctcctc gcgttcgc
//
LOCUS       DASWAV010000545         2640 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U2665
            k141_711633, whole genome shotgun sequence.
ACCESSION   DASWAV010000545 DASWAV010000000
VERSION     DASWAV010000545.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960316
            Sequence Read Archive: SRR10260007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2640)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2640)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:47:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,929
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2640
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_711633"
                     /isolate="SMAG_U2665"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.36444 N 132.31889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            147..2375
                     /locus_tag="VF958_11455"
     CDS             147..2375
                     /locus_tag="VF958_11455"
                     /inference="COORDINATES: protein motif:HMM:NF013123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HEX9149767.1"
                     /translation="MRFRAPSIAATAAAAILLLPTPGSAQKRKLSIEDLTAEPPIAGR
                     PVSRVTWIGKGERFSYLARKGSDETAVSELWVEKTATGQKKMIVSTSGLALPEEPEPA
                     KGPPPGEKTVERPRTASLDGYAWSPDGRRVLVNGGDDLWLYDAVAGRLERLTRTAGRE
                     ELPSFSPDGARVAFVRNNDLYAVELDSSRETRLTRDGGALVYNGKLDWVYEEELAGRD
                     ARGYEWSPDGRSIAYLKLDDAPIAPSPIVDFLAVPANVNWQRYPKAGAKNPVASFHVV
                     GVDGKERAAVAAENDSYIVPGFSWTPDSAAVCYRVLNRAQNREEVRLLALAPAGAPSR
                     TLFVEEDKYWLNVFEPPRFLRGGRYLWKSERAGFAHLYVGRDSGGEPQPITHGEWIVD
                     KIAGVDESRGNVYFTATEENVRRRPIYRVSLDGRGFTKVTTTPRGQHSPELSPDGKFL
                     LDTFSTIDQPPVLSLLDPAGGTARVVDDPAGRLGEFELGKNEEVEVSGDDGARLEGHL
                     LKPADFDPTKKYPVIVYVYGGPHSQVVRDAWGATTFLDHLLASRGFLVWSLDNRGAFG
                     RGHAWESAIFHDMGRRELSDQLAGVRYLKSLPFVDASRVGIWGWSYGGYLTLYSMTQA
                     PDVWKCGIAGAPVTHWKFYDTIYTERYMGTPQENPDGYERSAPLSKAGDLAAPLLIVH
                     GGSDDNVHVQNTVAFVDALVRAGKPYELQVQPRQKHGFRGAESLNFRNRAIVRFFEEN
                     LH"
BASE COUNT          458 a          885 c          883 g          414 t
ORIGIN      
        1 cgccggcgag ttctcgcgcg atcgaatggt tcctgcgttc ggcaacgtcg gcttctcgct
       61 cgcgcccgga gagatcggga tggccgagta cgacccgcgg acaagcccct acggctggca
      121 catcatcaag cggctcgagt agaacggtga gattccgcgc gccgtcgatc gccgcgaccg
      181 ccgcggcggc gatcctcctt cttccgacgc caggctcggc gcagaagagg aagctctcga
      241 tcgaggactt gacggccgag cctccgatcg ccggccggcc agtatcccgc gtgacgtgga
      301 tcggaaaggg cgagcgattt tcgtacctcg cgcgcaaggg ctccgacgag accgcggtat
      361 cagagctctg ggtcgagaag accgcgaccg gccagaagaa gatgatcgtc tcgacatccg
      421 gcctcgcgct cccggaggag ccggagccag cgaaaggacc gccacccggg gagaagacgg
      481 tggagcggcc acgcacggcg tcgctcgatg gatacgcctg gtcccccgac gggcggcgcg
      541 tgctcgtgaa cggcggcgac gatctctggc tctacgacgc ggttgccggc cggctcgagc
      601 gtctcacgcg tacagccggc cgcgaggagc ttccctcctt ctcgcccgac ggagcgcgtg
      661 tcgccttcgt gcgaaacaac gacctctacg cggtcgagct cgatagcagc cgcgagacga
      721 ggttgacccg cgatggcggc gccctcgtct ataacggaaa gctcgactgg gtgtacgagg
      781 aggagctcgc cggccgggac gctcgtggct acgagtggtc gccggacggc cgttcgatcg
      841 cctacctcaa gctcgatgac gcgccgatcg cgccgagtcc gatcgtcgat ttcctggcgg
      901 tccccgcgaa cgtcaactgg cagcgctatc cgaaggcggg agcgaagaac ccggtcgcat
      961 ccttccacgt cgtcggcgtc gatgggaagg agcgcgccgc cgtcgccgcc gaaaacgact
     1021 cttacatcgt gcccggattt tcgtggacgc cggactcggc ggctgtgtgc taccgggtcc
     1081 tgaatcgcgc gcagaaccgg gaggaagtcc gcctgctcgc gctcgctccg gccggcgcgc
     1141 cttctcgaac gctgttcgtc gaggaagaca agtactggtt gaacgtcttc gagccgccgc
     1201 ggttcctccg cggcggccgg tatctctgga aatccgagcg cgccggcttc gctcatctct
     1261 atgtcggccg tgattcgggc ggcgagccgc agccgatcac gcacggcgag tggatcgtgg
     1321 acaagatcgc cggcgtcgac gaatcacgcg ggaacgtcta cttcacggcg accgaggaga
     1381 acgttcggcg cagaccgatc taccgggtct ccctcgacgg gcgcggcttc acgaaagtga
     1441 cgacgacccc gcgcggccag cactcgccgg agctctcccc cgacgggaag ttcctgctcg
     1501 acacgttctc gacgatcgac cagccgccgg tcctctcact gctcgacccg gcgggcggga
     1561 ccgcgcgagt cgtcgacgac cccgcgggac gtctcggaga gttcgagctc ggaaaaaacg
     1621 aggaggtcga ggtctcgggc gacgacggcg cgcggctcga aggacatcta ctgaaacccg
     1681 ccgacttcga tccgacgaag aagtatcccg tcatcgttta cgtctatggc ggcccgcatt
     1741 cgcaggtcgt ccgggacgcc tggggagcga cgacgtttct cgatcacctc ctggcgagcc
     1801 gcgggttcct tgtctggagc ctcgacaacc ggggtgcgtt cggccgcggg cacgcgtggg
     1861 agtcggcgat cttccacgac atgggacgac gcgaactctc cgaccagctc gcgggagtcc
     1921 gctacctgaa gagcctcccg ttcgtcgacg catcgcgcgt cggcatctgg ggctggtcct
     1981 acgggggcta cctgactctt tactcgatga cgcaagcgcc cgacgtgtgg aagtgcggaa
     2041 tcgccggcgc cccggtcacc cactggaagt tctacgacac gatctacacc gagcgataca
     2101 tgggcacgcc gcaggaaaac ccggacggct acgagaggtc cgcaccgctc tcgaaggcgg
     2161 gcgacctcgc ggccccgctc ctcatcgttc acggcggatc cgacgacaac gtccacgtcc
     2221 agaacacggt ggcgttcgtg gacgccctcg tgcgggcggg caagccctac gagctccagg
     2281 tccagccgcg ccagaagcac ggcttccgcg gcgcggagtc tctgaacttc cggaaccggg
     2341 cgatcgtccg gttttttgaa gaaaatctcc actaaaccgt acgcaggcct ttcagtttcg
     2401 cggcgcgaga agtctccgcg aagtaaccga gcggaggagg ctcgcgccgc gggagcggga
     2461 ccgcggcgaa cgaccaggca tgcggtcgag gcccatttgc cgagccgcgt gccggtgcgc
     2521 agcaggtcgt tcgcaaggga ccgcgaacgg caaaaataac gccgccccga ttgctcgggg
     2581 cggcgtgttt tccggtcggt ccagccagtt tgtgcgaaag gcgcggtccg acgccatcaa
//
LOCUS       DASWAV010000546         2014 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U2665
            k141_511236, whole genome shotgun sequence.
ACCESSION   DASWAV010000546 DASWAV010000000
VERSION     DASWAV010000546.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960316
            Sequence Read Archive: SRR10260007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2014)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2014)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:47:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,929
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2014
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_511236"
                     /isolate="SMAG_U2665"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.36444 N 132.31889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            80..1048
                     /locus_tag="VF958_11460"
     CDS             80..1048
                     /locus_tag="VF958_11460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018307344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HEX9149768.1"
                     /translation="MKFLVFIEQRDGKIRKASLEALSLARRLAGEPVAAVIAGSGVTP
                     LAAGLGAQGAARVHVADRPDLELYSNKGYVGALDAARKKENPDAVLIAATAMGKDVAP
                     RFAARNDVSVLADVMDLSREGDRLVASRPVYSGKARAQVDCGAALLQVATTRPNVFPV
                     EVDGTAPAAQVEPLELAEVASRARVLRVETSESKELDVAEADIIVSGGRGIKGPENWP
                     VIRDLQQALGAALGASRAVVDAGWIDHQYQVGQTGKVVSPTLYVACGISGAIQHLAGM
                     GTSKVIVAINKDPEAPIFKVATYGIVGDIFQIVPEVAKAVRAAKAE"
     gene            1229..>2014
                     /locus_tag="VF958_11465"
     CDS             1229..>2014
                     /locus_tag="VF958_11465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9149769.1"
                     /translation="MPPPPTERFLLGLPGTWLFGLLLLFATAAFVYSISRRVRVLLAT
                     GPENRFEKIGTRIAKTLEFAFAQKRMFRDSYAGTFHILIFSGFLVLLVRTIALVVEGL
                     APGFVLLRGPAGDAYTLAKDVFEILVLVGVTMAVFRRAFARPRRLDLTADAWFILFLI
                     ALLIVADLVSEGARVALAPSLASEWSPAVLAVAGMMQGLPRETLQGIYEAGWWTHLLD
                     ILFFGNYLPYSKHFHIITSIPNVFFMKLEPMGRLGTPNLETAEH"
BASE COUNT          308 a          699 c          672 g          335 t
ORIGIN      
        1 gcccctctcc cggagggaga gggagttcct gtggcgccgc ggaccgcttc gcgacgcggc
       61 gcgatcgagc aattgagccg tgaaattcct cgtcttcatc gagcagcgcg acgggaagat
      121 ccgcaaggca tctctcgaag cgctttcgct cgcacgcagg ctcgcaggcg aaccggtggc
      181 cgcggtgatc gcgggaagcg gagtgacgcc cctcgcggcg ggtctcggcg cccagggtgc
      241 ggcccgcgtc cacgtcgccg accggccgga cctcgagctc tacagcaaca agggctacgt
      301 gggcgcgctc gacgctgcga ggaagaagga gaatcccgac gccgtcctga tagccgcgac
      361 ggctatgggc aaggacgtcg cgccgaggtt cgccgcgcgc aacgacgtct cggtcctcgc
      421 cgacgtgatg gatctgtcgc gcgaagggga ccgcctcgtg gcgtcgcggc cggtttactc
      481 cggaaaggcg cgagcgcagg tcgactgcgg cgcggctctc ctccaggtcg cgacgacgag
      541 acccaacgtc ttcccggttg aggtcgacgg gacggccccc gccgcgcagg tggaaccgct
      601 cgagctggcc gaggttgcct cccgggcccg cgttctgcgc gtcgagacct cggagtcgaa
      661 ggagctcgac gtcgccgagg cggacatcat cgtctccggc ggccgcggca tcaaaggccc
      721 cgagaactgg ccggtgatcc gcgatctcca gcaggcgctc ggcgccgccc tcggcgcgtc
      781 gcgcgccgtc gtcgacgcgg ggtggatcga ccaccagtac caggtcggac agaccgggaa
      841 ggtcgtgtcc ccgacgctct acgtcgcgtg cggcatatcc ggcgcgatcc agcacctcgc
      901 cggaatggga acctcgaaag tcatcgtcgc gatcaacaag gacccggagg cgccgatctt
      961 caaggtagcg acctacggga tcgtcgggga tatcttccag atcgttccgg aggtggcaaa
     1021 ggcggtgcgc gccgcgaagg cggagtgatc cgtcgcgctc gctgcgcacg agatccggct
     1081 atgccaccgg cgctcggctc ggcaagcgag cctcgaccga gcgcctgatt cctcgcgccg
     1141 agagcagaag aggaaaattc accgagccgc ttgccggtga gacgcaggta ggtcgccagg
     1201 gagcgcgacg gtcggttaga gttcatccgt gccgccgccg ccgaccgagc gcttccttct
     1261 cggactcccg ggcacgtggc tcttcgggct tcttctcctc ttcgcgaccg cggcgttcgt
     1321 ctactccatc tcgcggcgcg tgcgcgttct gctcgccacc ggcccggaga accgcttcga
     1381 gaagatcggg acccgcatcg cgaaaacgct cgagttcgca ttcgcccaga agcggatgtt
     1441 ccgggactcc tacgcgggca cgttccacat cctgatcttc agcggtttcc tcgtgttgct
     1501 cgtccgcacg atcgccctcg tcgtcgaggg gctcgcgccc gggttcgttc tcctccgcgg
     1561 cccggcagga gacgcctaca cgctcgccaa ggacgttttc gagattctcg tgctcgtcgg
     1621 ggtgacgatg gcggtcttcc ggcgcgcgtt cgcgcgcccc cgccgcctcg acctgaccgc
     1681 ggacgcctgg ttcatcctgt tcctgatcgc gctcttgatc gtggcggacc tcgtctcgga
     1741 gggcgcacgg gtggccctgg cgccgtcgct cgcgagcgag tggtcgccgg cggtgctcgc
     1801 ggtcgcgggg atgatgcagg gccttccccg cgaaaccctt cagggaatct acgaagcggg
     1861 ctggtggacg cacctcctcg acatcctttt cttcggcaac tacctgccgt actcgaagca
     1921 cttccacatc atcacgtcca tccccaacgt cttcttcatg aagttggaac ccatgggccg
     1981 gctcggcacg cccaacctcg agaccgcgga gcac
//
LOCUS       DASWAV010000547         2086 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U2665
            k141_350148, whole genome shotgun sequence.
ACCESSION   DASWAV010000547 DASWAV010000000
VERSION     DASWAV010000547.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960316
            Sequence Read Archive: SRR10260007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2086)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2086)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:47:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,929
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2086
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_350148"
                     /isolate="SMAG_U2665"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.36444 N 132.31889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..912
                     /locus_tag="VF958_11470"
     CDS             <1..912
                     /locus_tag="VF958_11470"
                     /inference="COORDINATES: protein motif:HMM:NF024574.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9149770.1"
                     /translation="YWHRLNGVFAVWGARVRPSKTRAKLSTFDVAPTVLALLGLPADR
                     RMTGKAASGAFEGVTAPSRKDLFPTVAVRRVSSAPMSGEQASEYSKKLRALGYLTGGD
                     AQPVAAAGGDRPGMTEGAYNNLGVYLRETPRDLKAAEAALLKALELRPGYGSPIFNLA
                     VLYRMKGDDAKAIEWLFRSLDSGHSEPERTLTDWIVEYEEKGKTRPARVLLEHGVQRF
                     PENEAFARELALQKFKTSRDCPGAFATLSSFETKTTDTDTLNALGLFQTCLGRHREAI
                     ALFERSLALKPDQPAVVQSLNIVRRGI"
     gene            938..2071
                     /locus_tag="VF958_11475"
     CDS             938..2071
                     /locus_tag="VF958_11475"
                     /inference="COORDINATES: protein motif:HMM:NF024853.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HEX9149771.1"
                     /translation="MKKIRWVAIFLSLTFVAMGPAPAKGKRAPAASGGAGVERRLPEF
                     ANDYLPYEPDSRVAVQKADLKLPGFTSWKITRTGRYESLKAEATVFVSNDGKWFYEGD
                     SFLNRNPRPVRSAGDLDWVTSRYAELLHANVRAELAPDRDAGGLKGIFVVIETGYFRV
                     RLPGLVTPDGSVYLAGTLWDFQGDPRAERRRRIDLTAQRAQGSLDAPVTIVEFADMEC
                     GYCRFRGRQLDKLLAANSGGVNVRRHYKFFPLWSHHAWAMKAASAGDCLSRLANPAAL
                     FRFKDLVYSRQDSLSVSTIDELSITSAEGEGISRPDFLGCYLRDDSFDRVRKDLEEGY
                     RLNVNSTPTYFVDGTQINWVEDKVMEDYLRTKFPRIKTITYEK"
BASE COUNT          359 a          673 c          703 g          351 t
ORIGIN      
        1 tactggcacc gattgaacgg cgtgttcgct gtgtggggag cccgcgtgcg gccgtcgaag
       61 acgcgcgcga agctctcgac cttcgacgtc gcgccgaccg tgctcgccct gctgggcctg
      121 ccggccgacc gccggatgac cggcaaggcg gcgagtggcg ctttcgaggg cgtcaccgct
      181 ccttcccgga aggacctgtt cccgacggtt gccgtgcggc gcgtctcgtc ggcgccgatg
      241 tcgggagagc aagcgagcga gtattcgaag aagctccggg cgctcggcta cctgaccggg
      301 ggcgatgcgc agccggtcgc ggcggccggt ggagaccggc ccggcatgac cgagggcgcg
      361 tacaacaacc tcggggtcta tctgcgcgaa acgcctcgcg acctgaaggc ggcggaggcg
      421 gccctcctga aggcgctcga gcttcgtccg ggctacggct caccgatctt caacctcgcc
      481 gtgctctacc ggatgaaggg ggacgacgcg aaggcgatcg agtggctgtt ccgatccctc
      541 gactccggcc attcggagcc ggagcggacc ttgaccgact ggatcgtcga gtacgaggag
      601 aaggggaaaa cccgtcccgc gcgagtgctc ctggaacacg gggtgcagcg gttccccgag
      661 aacgaggcgt tcgcgcgcga actggcgctt cagaagttca agacctcgag agactgcccg
      721 ggagcgtttg cgacgctctc ctctttcgag acgaagacaa cggacaccga cacgctgaat
      781 gcgctcgggt tatttcagac gtgcctgggc cgtcaccgtg aagcgatcgc gctcttcgaa
      841 cgctctctcg cgctcaagcc cgaccagccg gcggtcgttc agtcgttgaa tatcgtgcgg
      901 cgcgggatct agctacgcga caggagagaa tgagccgatg aagaagattc gatgggttgc
      961 gatttttctg tccctgacgt ttgtcgcgat gggccctgcg ccggcgaagg gaaagcgggc
     1021 gccggccgcg tcagggggtg cgggcgtcga gaggcggctt ccggagttcg cgaacgacta
     1081 ccttccgtac gagcccgact cccgggtcgc cgtgcagaag gcggatctca agctcccggg
     1141 attcacatcc tggaagatca cacgcaccgg ccgctatgaa tctctcaagg ccgaggcgac
     1201 cgtgttcgtc tcgaacgacg gcaagtggtt ctacgaaggc gactcgtttc tcaaccggaa
     1261 tccgcggccc gtgcggtcgg cgggggacct cgactgggtc acctcgcgtt acgcggaact
     1321 tctccacgcc aacgtgcgcg cagagctcgc tcccgatcgc gacgccggag ggttgaaggg
     1381 gatcttcgtc gtcatcgaga cgggatactt tcgggtccgg ctgccgggcc tcgtgacgcc
     1441 cgacggcagc gtctatctcg cgggaaccct ctgggatttt cagggcgatc cccgcgccga
     1501 acggcggcgt cgaatcgacc tgaccgcgca gcgtgcgcag gggagcctgg acgctcccgt
     1561 cacgatcgtc gaattcgccg acatggagtg cgggtactgc cgcttccgcg gccggcagct
     1621 cgacaagctc ctcgccgcca actcgggagg cgtgaacgtg cggcgccact acaagttctt
     1681 cccgctctgg tcccaccacg cgtgggcgat gaaggccgcc tcggccggag actgcctctc
     1741 gcggctcgcg aatcccgcgg cgctcttccg gttcaaggac ctcgtctaca gccgtcagga
     1801 cagcctttcg gtctccacga tcgacgagct ctcaatcacc tcggcggagg gcgaggggat
     1861 ctcgcgcccc gacttcctcg ggtgctacct gcgcgacgac agcttcgatc gcgtccgcaa
     1921 agacctcgag gagggctatc ggctgaacgt gaactcgact ccgacctact tcgtcgacgg
     1981 gacgcagatc aactgggtcg aggacaaggt gatggaggac tacctgcgga cgaagttccc
     2041 gcggatcaag acgatcacct acgagaagta gacgggtacc ggtcgc
//
LOCUS       DASWAV010000548         2215 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U2665
            k141_191711, whole genome shotgun sequence.
ACCESSION   DASWAV010000548 DASWAV010000000
VERSION     DASWAV010000548.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960316
            Sequence Read Archive: SRR10260007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2215)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2215)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:47:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,929
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2215
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_191711"
                     /isolate="SMAG_U2665"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.36444 N 132.31889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..568
                     /gene="lepB"
                     /locus_tag="VF958_11480"
     CDS             <1..568
                     /gene="lepB"
                     /locus_tag="VF958_11480"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: protein motif:HMM:TIGR02227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HEX9149772.1"
                     /translation="SMEKNILVGDHLIVNKFIFGPHRGGVLSRLLPYRAVERGDVIVF
                     KFPEDPRRDFIKRTIALPGETVEIRDKVIYVEGKRLVEPRIHHADERVWPDDPALPDS
                     YRRRDQSRPFTVPPDSYFALGDNRDNSYDSRFWGPVPAANLKGRAMFVYWSFPLEPST
                     HRSVRELIEISLNFFRRTRWERTLLPVR"
     gene            616..993
                     /locus_tag="VF958_11485"
     CDS             616..993
                     /locus_tag="VF958_11485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9149773.1"
                     /translation="MRRRGPRSRGAGNIGCLLWLVVVVAVGHVLWKVVPVKIRTSEFY
                     DVMQEQASFGSIKSEQQIQYEILRRAQELKIPVTKDNFKIVRSREAVTVEAHYEIPLE
                     FFGGLYRYVWKFNPVISRPTFAV"
     gene            1115..1696
                     /locus_tag="VF958_11490"
     CDS             1115..1696
                     /locus_tag="VF958_11490"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HEX9149774.1"
                     /translation="MRVVRSILVLAALGVLACNAVAEAPAIGAAAPDFQLTALDGKAF
                     SLSSAAKAHRAVVVMFISTQCPYSNAYNDRMKDMANAYASRGVQFVGINSNKTEDAAE
                     VASHAKSHGHTFPIMKDPDNKVADLYDARHTPTVYVVDSQGKLRYAGRIDDQTKAADV
                     TSPDLKNALESLLANQPIAKTTTKAFGCSIKRV"
     gene            1693..>2215
                     /locus_tag="VF958_11495"
     CDS             1693..>2215
                     /locus_tag="VF958_11495"
                     /inference="COORDINATES: protein motif:HMM:NF012314.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HEX9149775.1"
                     /translation="MSVPGPGFRVSAGRAIVALLVLSSLAAAPTSRLAGELDASALKQ
                     TVAREKGRVVLLNFWATWCVPCREEFPELSKLQTRNQEAGLRVIGVSTDFPKERAAVE
                     KFLVESRPSFPSYLKKSVGDDEDFINAVDSSWGGELPFSVLYDRVGKKVHVYSGSLPL
                     AAAEKEIRRLLAVR"
BASE COUNT          398 a          726 c          692 g          399 t
ORIGIN      
        1 ctcgatggag aagaacatcc tcgtcggcga tcacctgatc gtgaacaagt tcatcttcgg
       61 ccctcaccga ggcggcgttc tctcgcgcct tctcccctat cgggccgtcg agcgcggcga
      121 tgtcatcgtc ttcaagttcc cggaggatcc gaggcgggac ttcatcaaga gaacgatcgc
      181 actgccgggc gagacggtcg agatccggga caaggtgatc tacgtggaag gaaagcgact
      241 cgtcgagccg cggatccacc atgcggacga gcgcgtgtgg cccgacgacc ccgcgctgcc
      301 cgactcttac cgccgccgcg accagtcgcg gccgttcacc gtgccgccgg actcctactt
      361 cgccctcggg gacaaccgcg acaactcgta cgacagccgg ttctggggtc cggttccggc
      421 cgcgaacctg aaggggcgcg cgatgttcgt ctactggtcg ttcccgctgg agccgtcgac
      481 tcaccgctcc gtgcgcgagc tgatcgaaat ctcactcaat ttcttccgcc ggacgcgatg
      541 ggagcgcacc ctgctgccgg tgcggtgaac cgccggaatc cgggttaaga ttttggtcgc
      601 cttcagggag gatcaatgag acgcagaggg ccgcgttcac gcggcgcggg gaacatcggc
      661 tgcctgctct ggctggtcgt cgtggttgcg gtcggacacg tcttgtggaa ggtcgtcccc
      721 gtgaagatcc gcacgtcgga gttctacgac gtcatgcagg agcaggcgtc gttcggatcc
      781 atcaagagcg agcagcagat ccagtacgaa atccttcgcc gcgcccagga gctcaagatc
      841 ccggtgacga aggacaactt caagatcgtg cggtcgcgcg aggccgtgac cgtcgaggct
      901 cactacgaaa ttccgctcga gttcttcggg ggcctctacc ggtacgtctg gaagttcaac
      961 cccgtcatca gtcgacccac attcgcagtc tgacgcagaa agtcccgggt cttactcccc
     1021 ggtgcccgat cccggattcc aggttcccgg tcccaggagc cgggactaag ggcggcttcg
     1081 cccgcggttc ggccgctcga ggaggaagat cgccatgcgc gtcgtccggt ccatcctggt
     1141 tctcgcggcc ctcggtgtac tcgcctgcaa cgccgtcgcg gaagctcccg ccatcggcgc
     1201 cgccgcaccc gactttcagc tgactgcgct cgacggcaag gccttctctc tgtcgtccgc
     1261 cgcgaaggcc cacagggcgg tcgtcgtgat gttcatctcg acccagtgcc cgtactcgaa
     1321 cgcgtacaac gaccggatga aggacatggc gaacgcgtac gcgtcacgcg gagtccagtt
     1381 cgtcggaatc aacagcaaca agacggaaga tgcggcggaa gtcgcaagcc acgcgaaatc
     1441 ccacggtcac acgttcccga tcatgaagga cccggacaac aaggtggcgg atctctacga
     1501 tgcccgccac actccgaccg tctacgtcgt cgactcgcag ggcaagttgc gctacgcggg
     1561 tcggatcgac gaccagacga aagccgcgga cgtcacctcc cccgatttga agaacgcact
     1621 cgagtcgctg ctcgcgaatc agccgatcgc gaagaccacg acgaaggcat tcggctgctc
     1681 gatcaagcgg gtttgagcgt cccgggcccc gggtttcggg tctccgccgg aagggcgatc
     1741 gtcgcccttc tcgtcctctc ttcgctcgcc gcggctccga cgtcgcggct cgccggcgag
     1801 ctcgacgctt ccgcgctcaa acagacggtc gcgcgggaaa aggggcgtgt cgtcctgctc
     1861 aacttctggg cgacgtggtg tgtcccgtgt cgcgaggagt tcccggaact gtcgaagctg
     1921 cagacgcgga accaggaggc gggactccgg gtaatcggcg tttcgaccga cttcccgaag
     1981 gagcgggccg ccgtggagaa gtttctggtc gagagtcgcc cctcttttcc gagctacctc
     2041 aaaaagagcg tcggcgacga cgaggacttc atcaatgcag tggattcgtc gtggggaggc
     2101 gagctcccct tttccgttct ttacgaccgc gtcgggaaga aggtccacgt ctactcgggt
     2161 tctctgccgc tcgccgcggc cgagaaagaa atccgaaggc tcctcgcagt ccgct
//
LOCUS       DASWAV010000549         7546 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U2665
            k141_632129, whole genome shotgun sequence.
ACCESSION   DASWAV010000549 DASWAV010000000
VERSION     DASWAV010000549.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960316
            Sequence Read Archive: SRR10260007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 7546)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7546)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:47:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,929
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7546
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_632129"
                     /isolate="SMAG_U2665"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.36444 N 132.31889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(67..294)
                     /locus_tag="VF958_11500"
     CDS             complement(67..294)
                     /locus_tag="VF958_11500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9149776.1"
                     /translation="MLGSALFVFYCTAVGVYLVLRPWTGLFTGGGLTASGFFRGFVSG
                     LGLVHLVAGAADLKVLSHELSASAAGEDLVG"
     gene            complement(455..2950)
                     /locus_tag="VF958_11505"
     CDS             complement(455..2950)
                     /locus_tag="VF958_11505"
                     /inference="COORDINATES: protein motif:HMM:NF042645.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6600 domain-containing protein"
                     /protein_id="HEX9149777.1"
                     /translation="MSSTKRILLGVAMTLAMVATAFAQYEGASRPDSYRSDRGVERNG
                     YSYVRDTVGGVTVESRLNGRVTARRNMPISAGDEIFVSDAGRAEIGLADGNLLFVGGG
                     TRARFTSLYAQQGENDDFSAIDLKDGSVVLAAMGYDDQSIPRVDTEDATVYLSAGTRV
                     RVNADPRRGTVVVVRSGSAEVRTRLDSYTVEAGQYLMVEGDEPAEISRGVFSRDRFDL
                     WAADRLEDLSVVTAQSASARYVDDDYASDVVALDGYGDWQYSSTYSTNVWVPRVSVDW
                     TPYSYGSWYYTPIGLTWWSGDPWGWYPFHYGSWFFDAGFRHWCWAPAYTYSPAWVYWG
                     YSGRYVGWCPVGYYSFYSPWWDTYYRNWGWHSRHNGGLMFGISGSFRSKQIDLGGWNF
                     VNSGSFGSGSDRINVFPGKQIASNLSDQIAISSKAIVVSSRGEPISQAIQTFVKQAPS
                     MIERTSKGSSRLAPILAREKTLPAATLDELKGRAVVAGRGKLAGPGADEIASRGMLVD
                     RSRSVKDVLNSRGVEKGSIDGSGGSFRGNKAPDRVPGAETAREAPNVWGRGGQKIDKA
                     APGAAPAPSAGDWRVRGRGVEQPGKPSKEASPAPSAGDWRGRGPAVEKGERPAPEVAP
                     RDKGFDRPAPAPRSNDDWRGRGKSDAPPPQSDAPAREIERQKPAKPSARESDWRGRSE
                     TRFGGNAAPAREPGGQEWRRGDPPAKRVIEGAVPGRRWQDSGAAPRARESSPPAEWRQ
                     RGPERSAPAPREYKQAPAPREYRPEPRQYAPREYKQAPAPREYRPEYRPAPAPRAPQY
                     SAPPPAPRNDSRPAPAPKGDGGFSKGNGKGRKG"
     gene            3143..3652
                     /gene="def"
                     /locus_tag="VF958_11510"
     CDS             3143..3652
                     /gene="def"
                     /locus_tag="VF958_11510"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: protein motif:HMM:NF001159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HEX9149778.1"
                     /translation="MPIRDLVRWGDERLTAPNEEIVAPDGLAGLLSDLIATCHAAPGV
                     GLAAPQIGVNRRVAIVDLSVGEDPSAILVLVNPVVVSAVGEQKEEEGCLSVPDVSEKV
                     VRPARVVVRAADADLRVREIEGVGLLARALCHETDHLNGMLFLDRLRGLKKEMTRKKI
                     ERRRARSGW"
     gene            3646..5055
                     /gene="cysS"
                     /locus_tag="VF958_11515"
     CDS             3646..5055
                     /gene="cysS"
                     /locus_tag="VF958_11515"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020259281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HEX9149779.1"
                     /translation="MVKLTNTLGRRLVDFAPLQPGHVRLYTCGPTVYNVVHIGNLRTF
                     VFEDVLRRRFRARGDRVTQVMNLTDVDDKTIRGAQEAGLSLGEFTAKYAEAFFRDIGR
                     LNVEPAEHYPKATDHIPEMIEVIRKLEERGHTYGSEGSVYFRISTFPGYGKLSGIDLE
                     KTRRGDRVADDEYEKEDVKDFALWKAAKPGEPSWPSPWGQGRPGWHIECSAMSMKYLG
                     EHFDIHTGAVDNIFPHHENEIAQSEGATGHPFVEVWLHAEHLIVDGEKMSKSKGNFYT
                     LDDVLERRDDPAAVRYLFLSVPYRKKLNFTWEALGAAAAAVARIRSAAVRIDELASAA
                     TPPRPGSFPSEERAALFRSEFASGMDDDLNTPEALAAVFNYLRQVNAAVDDGSLDSDG
                     ARVARGALADADRVLGVLPEAPEVLSSEVEEKIAARNAARLRRDFAEADRIRDELGAR
                     GIVLEDTPGGTRWKKASGV"
     gene            5165..6688
                     /locus_tag="VF958_11520"
     CDS             5165..6688
                     /locus_tag="VF958_11520"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: protein motif:HMM:NF013640.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003855 - 3-dehydroquinate dehydratase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HEX9149780.1"
                     /translation="MKRPPGPEGTGGFSFLRERQLLLPAHVASIAPRDMADAKSLLAR
                     APSRADAIEFRLDGSVERIPVAALLALDPRPVIVTWRGAREGGHFDGSLEEYRRLVLE
                     AYAAGAIVDVEHASGVAGDPGLTDAKRVVVSAHFPFGLPADWEPRLAAMKATGPVVVK
                     FVAGAAHLAASLAIARIAASRSDGSTAIFPMGPASLPGRILAALSGGALVYGAVGGPT
                     APGQPLLSELFDVYGTDRKRRPDALFGIVAVDPTGSLSPPVHNALFRSRDLSSVYLPL
                     PVADFEREMPHDLAFDPPFRGFSVTRPWKLAAARAGAAGEDVRATGAANTIFREGRRW
                     RVENTDVDGVFDPLADYDTGEGRAAVILGAGGVARAAIVAAKRLGYEVAVAARHGGDA
                     DALAVKFGVGTVAWESVAETEADLYVNATPVGWRPEDPPAVSARVLAARPLVFDCVYR
                     RGGGETATIRAAREAGCRTVDGLEMFAVQAAAQARLFGIEGVTREEVGRILRERRAA"
     gene            6685..>7546
                     /locus_tag="VF958_11525"
     CDS             6685..>7546
                     /locus_tag="VF958_11525"
                     /inference="COORDINATES: protein motif:HMM:NF016593.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate-binding protein"
                     /protein_id="HEX9149781.1"
                     /translation="MTPGGDRGRIVPVSRDLEGLDVTPMDLFRTMRGSGQECFLLESV
                     EGGEAIARYTFLGVAPSARLTARGEEIEIERGGICRRSRLPLLPALEALTLRERYEPD
                     PDLPPFCGGAVGYLGYDAARLFEEIPDRHAPESGMPDALFLRFDAIVAFDRARGRILL
                     TTIAEEGETEAAQESLNRLEELLSRTTPHQAHPLPGRAERGSAVDFRPAMPRKDFLAA
                     VEAAKEAILAGEIYQVVLSQRWTAPLSIDPLDVYQSLRTLNPSPYLFFLHTREAAVFG
                     ASPEMLVRCRG"
BASE COUNT         1176 a         2576 c         2569 g         1225 t
ORIGIN      
        1 gggcttcgag gacgtgctcg aagcgcgcct cgccgatcgc ggcgcgatcg gtgacgtaga
       61 gaagcctcac ccgaccaggt cctcgcccgc ggcggaggcg gagagctcgt gcgaaaggac
      121 cttcagatcg gcggctccgg cgacgaggtg caccaggccg agacccgaca cgaaaccgcg
      181 aaaaaagccg gaggccgtca agccgcctcc cgtgaagagg ccggtccacg ggcgcaggac
      241 gagatacacg ccgaccgccg tgcagtagaa gacgaacagg gcgctgccca gcactgtcga
      301 aggtctcagg cgcattagcc acgaattgta gtcggcgcgg cccggcgccg aggtgggcgt
      361 cttcacttcg cggaaccggg gctcagattc ccggcgtcaa aaaaaaccgc gcccgagggc
      421 gcggttgcga agaggaaatc cccgaggagt cgagtcagcc ttttcgaccc ttgccgttac
      481 ccttgctgaa gcccccgtcc cccttcggtg cgggagccgg gcgcgagtcg ttccgtggtg
      541 caggtggggg cgccgagtat tgcggggcgc ggggcgccgg tgccggccgg tattccggac
      601 ggtactcgcg cggcgcggga gcctgcttgt actcgcgcgg cgcgtactgc cgcggttccg
      661 gacggtactc gcgcggcgcg ggagcctgct tgtactcgcg cggcgcggga gccgagcgtt
      721 ccggaccacg ctgacgccac tccgccggcg gggagctctc cctcgcgcgc ggtgctgccc
      781 cggagtcctg ccagcgccgg cccggaacgg cgccttcgat cactcgcttg gcaggaggat
      841 cgccgcgccg ccattcctgt ccgccgggtt cgcgagcggg ggcggcattc cccccgaagc
      901 gggtctccga gcggccccgc cagtcgctct cccttgccga cggcttcgcc ggcttctgtc
      961 gctcgatctc acgcgccggt gcgtcactct gcggaggcgg ggcgtcgctc tttccgcgac
     1021 cccgccaatc gtcgttggat cgtggcgcgg gagcggggcg atcgaatccc ttgtcgcgag
     1081 gtgcaacttc gggagcaggc cgttcgccct tctcgaccgc gggcccccgg ccgcgccagt
     1141 ctcctgccga cggtgccggt gaagcctcct tcgacggttt cccgggctgc tcgacgccgc
     1201 gtccccggac ccgccagtca cccgcggagg gagccggagc cgctcccggc gcagccttgt
     1261 caatcttctg tccccctcta ccccagacgt tcggagcctc ccgggcggtt tccgcgccag
     1321 ggacccggtc cggcgccttg tttccgcgga aggaacctcc gctgccgtcg atggagcctt
     1381 tttcgactcc gcggctgttg aggacgtcct tcaccgaccg gctccggtcg accagcatcc
     1441 cgcgcgacgc gatttcgtcc gcacccggtc cggccagctt gccccgaccg gcgacgaccg
     1501 cccggccctt cagctcgtcg agcgtcgcgg cagggagcgt cttctcacgc gcaagtatcg
     1561 gagcgagtct cgaagagccc ttgctcgtgc gttcgatcat cgagggcgcc tgcttgacga
     1621 acgtctgaat cgcctgcgag atgggttcgc ctcgggaaga gacgacaatc gccttggacg
     1681 agatcgcgat ctgatcgctc agattcgaag cgatctgctt tcccgggaag acgttgatcc
     1741 tgtcggagcc cgacccgaac gagccggagt tcacgaagtt ccagcctccc aggtcgatct
     1801 gcttcgaccg gaacgagccc gagatgccga acatgaggcc gccgttgtgg cgggaatgcc
     1861 atccccagtt tcgatagtag gtgtcccacc agggcgaata gaacgagtag tagcccaccg
     1921 gacaccatcc gacgtacctc ccggagtacc cccagtagac ccatgcggga gagtacgtgt
     1981 acgccggcgc ccagcaccag tggcggaatc cggcatcgaa aaaccagctc ccgtagtgga
     2041 agggatacca gccccacgga tcccccgacc accaggtcag accgatcggt gtgtagtacc
     2101 agctcccgta ggagtaaggc gtccagtcga cggagactcg aggcacccac acgttggtgg
     2161 agtaggtcga gctgtactgc cagtcaccgt agccgtcgag agcgacgacg tcgctcgcgt
     2221 agtcgtcgtc cacgtagcgc gccgaggcgc tctgcgccgt cacgaccgac aggtcttcca
     2281 ggcggtccgc cgcccagaga tcgaaacggt cgcgggaaaa gacaccgcgg gagatctcgg
     2341 ccggctcgtc accctcgacc atcaggtatt gccctgcttc cacggtgtag ctgtcgagtc
     2401 gcgtccgcac ctcggcggac ccggaccgca cgacgaccac ggttccgcgg cgcgggtctg
     2461 cgttgacccg gacccgcgtc ccggcggaga ggtagacggt cgcgtcttct gtgtcgactc
     2521 gcgggatcga ctggtcgtcg tatcccatcg cggcgagaac caccgacccg tccttgaggt
     2581 cgatcgcgga gaagtcgtcg ttctcgccct gctgcgcata gagtgacgtg aagcgggcgc
     2641 gcgtgcctcc gccgacgaaa aggaggtttc cgtcggcgag tccgatctcg gcgcggcccg
     2701 catcggagac gaagatttca tcgccggcgg agatgggcat gttgcggcgt gcggtgaccc
     2761 gtccgttcaa gcgggactcg acggtcacgc ccccgacggt gtcgcgcacg tacgaatagc
     2821 cgttgcgttc cacgccgcga tccgatcggt aggaatcggg tcgcgaagca ccctcgtatt
     2881 gggcgaaggc ggtcgcgacc atagcgagcg tcattgcgac gcccaggaga attcgtttcg
     2941 tggagctcat cggtatccct ccggtcgata gagatagcga tgcccgtgcc acgcgtgccg
     3001 ggcgtgtcga cgacggtaac ccgctgattt ccaatgcgtt gtccaagtgc cgatcgctcg
     3061 cgcctcgcgg gtgtgtccgg catggcggac aggggttcga cgtacgagga cactcgctgc
     3121 gaccctgcta gtctccccgt ccatgccgat ccgggatctc gtgcgctggg gagacgagcg
     3181 cctgactgcg cccaacgagg agatcgttgc ccccgacggc ctcgccgggt tgctgtcgga
     3241 cttgatcgca acctgccacg ccgcccccgg ggtgggtctc gccgcgcctc agatcggagt
     3301 caaccgccga gtggcgatcg tggatctctc cgtcggggag gatccctccg ccatcctggt
     3361 cctggtcaac ccggtggtcg tctcggcggt gggcgagcag aaggaagaag agggctgcct
     3421 ctccgtgccg gacgtctccg agaaagtggt ccgaccggcg cgggttgtcg tacgcgcggc
     3481 cgacgcagac ctccgcgtgc gcgaaatcga gggggtcggt ctgctcgcgc gggcgctctg
     3541 ccacgaaacg gatcacctga acggaatgct cttcctcgac cgccttcggg gtctcaagaa
     3601 ggagatgacc cgaaagaaga tcgagcgccg acgcgccagg agcggatggt gaagctgacc
     3661 aacactctcg ggcgccggct cgtcgacttc gcgccgctcc aaccgggcca cgtgcgtctc
     3721 tacacgtgcg ggccgaccgt gtacaacgtc gttcacatcg gcaacctgcg gaccttcgtc
     3781 tttgaagacg tcctccggcg tcgctttcgt gcgcggggcg accgggtgac gcaggtaatg
     3841 aacctgaccg acgtcgacga caagacgatc cgcggcgcgc aggaggccgg tctctctctt
     3901 ggcgagttca ccgcaaagta cgcagaagcg ttcttccgcg acatcggtcg gttgaacgtg
     3961 gagcccgccg agcattatcc gaaagccacc gaccacattc ccgagatgat cgaggtgatc
     4021 cggaagctgg aagagcgcgg acacacctac ggatccgagg gctccgtcta tttccggatc
     4081 tccacgtttc ccgggtacgg aaaactctcc gggatcgacc tcgagaagac gcggcgcggc
     4141 gatcgcgttg ccgacgacga atacgaaaaa gaagacgtga aggacttcgc cctgtggaag
     4201 gcggcgaaac cgggggagcc atcgtggccg tccccgtggg gacaggggcg gccggggtgg
     4261 cacatcgagt gctccgcgat gagcatgaaa tacctgggcg agcacttcga catccacacg
     4321 ggcgccgtcg acaacatttt tccgcatcac gagaacgaga ttgctcagag cgaaggggcg
     4381 accggccatc cgttcgtcga ggtctggctt cacgccgagc acctgatcgt cgacggcgag
     4441 aagatgtcga agtcgaaggg gaacttctac acgctcgacg acgtgctcga gcgccgggac
     4501 gatccggccg cggtgcggta cctctttctc tccgttccct accggaagaa gctgaatttc
     4561 acgtgggagg cgctcggggc ggccgccgcg gcagtcgccc gcatccgttc ggccgccgtg
     4621 cgcatcgacg agctcgcatc cgccgcgaca ccgccgcggc ccggctcgtt cccgtcggag
     4681 gagcgtgccg cgctcttccg ctccgagttc gcttccggca tggacgacga cttgaacacc
     4741 ccggaggcat tggccgcggt cttcaactat ctccggcagg tgaacgccgc cgtcgacgac
     4801 ggatcgctcg actcggacgg cgcacgagtc gcgcgcggcg ccctcgccga tgccgatcga
     4861 gttctcgggg tcctgccgga agcgccggag gtcctttctt ccgaggtcga ggagaagatc
     4921 gccgcacgca acgccgcccg cctgcgccgc gatttcgccg aggccgatcg catccgcgac
     4981 gaactcggcg cccgcggaat cgtcctcgag gacacgcccg gcggtacgcg gtggaagaag
     5041 gcgtccggcg tctgatatcc tcgcggcgat gactcgctct tcgatctggt gctggcggga
     5101 gggacatcgg tagggcgaat tcccgccctg ccggaaatcc ttttcgggcg gggcgaaaaa
     5161 cgacgtgaag agacccccgg gcccggaagg gaccgggggt ttttcgttct tgagagagag
     5221 acagcttctc cttcccgcgc acgtcgcatc gatcgcgccg cgcgacatgg cggacgcgaa
     5281 gagcctcctc gcgcgagcgc cctcgcgggc ggacgcaatc gagtttcgcc tcgacgggtc
     5341 ggtcgagcgg atccccgttg cggcgctcct cgctctcgac ccccgaccgg tcatcgtcac
     5401 ctggcgcggc gcgcgggagg ggggacactt cgacgggtct ctcgaggaat accgccgcct
     5461 cgttctcgag gcgtacgccg caggtgcgat cgtcgacgtc gagcacgcga gcggcgtcgc
     5521 gggcgatccg ggtctcaccg acgcgaagcg ggtcgtcgtg tcggcgcact ttcctttcgg
     5581 cctgccggcc gactgggagc cgcggctcgc ggcgatgaag gcgaccggac cggtcgtggt
     5641 caagttcgtc gccggcgccg cccacctcgc ggcaagcctg gcgatcgcgc ggatcgcggc
     5701 gagccgttcc gacggctcga cggcgatttt cccgatgggt ccggcaagcc ttcccggccg
     5761 cattctcgcg gccctatcgg gcggggcgct cgtctatggc gccgtgggcg gaccgaccgc
     5821 tccggggcag ccgctcctct cggaactctt cgacgtctac gggaccgatc ggaagcggcg
     5881 gcccgacgcg ctcttcggaa tcgtcgcggt cgatccgacc ggctcgctct cgcctcccgt
     5941 gcacaacgcg ctcttccgct cgcgggacct ttcatccgtg taccttccgc ttccggtcgc
     6001 ggacttcgag cgcgagatgc cgcacgacct cgcgttcgat ccgcccttcc gcgggttctc
     6061 ggtcacccgt ccctggaagc tcgcggcggc ccgcgccgga gcggcgggag aggatgtgcg
     6121 cgcgaccggc gccgccaaca cgatcttccg ggagggccgg cgctggcgcg tcgagaacac
     6181 ggacgtggac ggcgtgttcg acccgctcgc ggactacgac acgggtgagg gccgcgccgc
     6241 cgtaatcctc ggggcgggcg gcgtggcgcg cgccgcgatc gtcgccgcga agcggctcgg
     6301 ctacgaagtt gccgtcgcgg ctcgtcacgg cggagacgcg gacgcgctcg cggtcaagtt
     6361 cggcgtcggc acggttgcct gggagagcgt cgccgagacc gaggccgacc tctacgtgaa
     6421 cgccaccccg gtcggctggc gccccgagga cccgcccgcc gtttctgcga gagtcctcgc
     6481 cgcccggccg ctcgtctttg actgcgtgta ccgccgcgga ggcggggaga cggcgacgat
     6541 tcgcgccgcg cgggaagccg gctgccgcac ggtggacggc ctcgagatgt tcgccgtgca
     6601 ggccgcggcg caggcgcgcc tcttcggcat cgaaggcgtc acacgcgaag aggtcggacg
     6661 catcctccgg gaaaggagag cggcgtgaca ccgggcggag atcgcggacg gatcgttccg
     6721 gtgtcccgcg atctcgaggg actcgacgtg acgccgatgg accttttccg gaccatgcgc
     6781 ggctcgggtc aggagtgctt cctgctcgag agcgtcgaag gaggagaggc gatcgcgcgg
     6841 tacacgtttc tcggcgtcgc gccgtcggca cgcctgacgg cccggggaga ggagatcgag
     6901 atcgagagag gggggatctg ccggcgatcc cggctgccgc tccttcccgc gctcgaggct
     6961 ctcacgcttc gcgagcggta cgaaccggac cccgacctgc cgcccttctg cggcggtgcg
     7021 gtcggctatc tcggctacga cgccgcgcgc ctcttcgagg agatcccgga tcgccacgcc
     7081 ccggagagcg gcatgccgga tgcgctcttc ctccgtttcg acgcgatcgt cgccttcgac
     7141 cgcgcgcgcg gtcgcatcct cctgacgacg atcgcggaag agggggaaac ggaggccgcg
     7201 caggaatctc tgaatcggct ggaagagctt ctttcgagga ccacccctca tcaagcccat
     7261 cctctccctg ggcgcgccga gaggggctcg gccgtcgatt tccggccggc catgccgagg
     7321 aaggactttc tcgccgcggt cgaagcggcg aaggaggcga ttctcgccgg cgagatctac
     7381 caggtggtgc tctcgcagcg atggacggcg cctctctcca tcgatccact cgacgtctac
     7441 cagtcgctgc gaacgctcaa tccgtctccg tacctcttct tcctgcacac ccgggaagca
     7501 gcggttttcg gcgcctcgcc cgagatgctc gtgcggtgtc gcggga
//
LOCUS       DASWAV010000550         1847 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U2665
            k141_311749, whole genome shotgun sequence.
ACCESSION   DASWAV010000550 DASWAV010000000
VERSION     DASWAV010000550.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960316
            Sequence Read Archive: SRR10260007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 1847)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1847)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:47:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,929
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1847
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_311749"
                     /isolate="SMAG_U2665"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.36444 N 132.31889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            52..708
                     /locus_tag="VF958_11530"
     CDS             52..708
                     /locus_tag="VF958_11530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020709322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase cytochrome b subunit"
                     /protein_id="HEX9149782.1"
                     /translation="MGCIRAFWRSLVGKKVIMAVTGVILLLYIVGHLLGNLQIFEGPE
                     RLNAYARFLKSTGELLWAVRIVLLLSLVLHIIASVQVSLASKRARPADYEKKESIETS
                     YAARTMIWSGPLIFLYVVYHLAMFTFLVTGPGYSPTDVYRNEVQAFQVPAISLFYVVA
                     IVFLGMHLYHGAWSMLHTLGVSNPRYRFLRKTIAPVVAIAITAGYIAIPIAVLMGFIS
                     "
     gene            723..>1847
                     /gene="sdhA"
                     /locus_tag="VF958_11535"
     CDS             723..>1847
                     /gene="sdhA"
                     /locus_tag="VF958_11535"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011421689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase (quinone) flavoprotein
                     subunit"
                     /protein_id="HEX9149783.1"
                     /translation="MDLNGNIPGGPIEKKWDQRRFEMKLVNPANKRKYRVIVVGSGLA
                     GAAAAASLGQLGYEVLCFCYQDSARRAHSIAAQGGINAAKNYQNDGDSVFRLFYDTIK
                     GGDFRSREANVYRLAEISAAIIDQCVALGVPFAREYSGYLANRSFGGAQVSRTFYARG
                     QTGQQLLLGAYAALSREIAVGQVQMFPRSEMLDLVVADGRARGIVVRDLVTGEVTCHS
                     ADAVLLATGGYTNVYNLSTNAVGCNVTAIWRAHRRGAFFANPCFTQIHPTCIPVAGEH
                     QSKLTLMSESLRNDGRIWVPVKAGDPRHPGEIPEAERDYFLERRYPTFGNLVPRDVAS
                     RAAKAVCDEGRGVGPGRRGVYLDFADAFSRLGEDVIRERYE"
BASE COUNT          323 a          596 c          587 g          341 t
ORIGIN      
        1 atgccgctgg ccctgttgac gcggatcgga acacgatata ggtagagcaa tatgggatgt
       61 attcgagcgt tttggcggag cctcgtcggg aagaaagtga tcatggccgt taccggcgtg
      121 atcctcctgc tctacatcgt cgggcacctg ctcggtaatc tgcagatctt cgagggtccg
      181 gagcggctga acgcctacgc caggttcctg aagagcactg gagagctcct ctgggcggtg
      241 cgaatcgtgc tgctcctctc cctcgtcttg cacatcatcg cttcggtaca ggtctcgctg
      301 gcgagcaagc gggcccgacc tgccgactac gaaaagaaag agagtatcga gaccagctac
      361 gcggcgcgca ccatgatctg gagcggcccg ctcatcttcc tgtacgtcgt ctaccacctg
      421 gcgatgttta cgttcctggt gaccggccct ggatacagcc cgaccgacgt ttaccgcaac
      481 gaagtgcagg cgttccaggt gccggcaatc tccctcttct acgtcgtggc gatcgtcttc
      541 ctcggcatgc acctctacca cggagcctgg agcatgctgc acactctcgg agtgagtaat
      601 ccgaggtacc ggtttctccg aaagaccatc gcgccggtcg tggcgatcgc gatcacggcc
      661 ggctacatcg ccattccgat cgcggtcctg atgggcttca tttcatgact gcggagccgc
      721 aaatggattt gaacggcaac atcccggggg gcccgatcga gaagaagtgg gatcagcgac
      781 gcttcgagat gaagctcgtc aacccggcaa acaagcgaaa gtacagggtc atcgtcgtgg
      841 gatcggggct cgcgggcgcc gcggcggccg cctcgctcgg gcagctgggt tacgaggtgc
      901 tctgcttctg ttaccaggac agcgcacgcc gcgcgcactc gatcgccgcg cagggcggga
      961 tcaacgcggc gaagaactac cagaacgacg gcgacagcgt cttccggctc ttctacgaca
     1021 cgatcaaggg cggtgacttc cgttcccgcg aggccaacgt ctaccggctg gcagaaatct
     1081 cggcggcgat catcgaccag tgcgtggccc tgggagtgcc cttcgcacgg gagtacagcg
     1141 gctacctcgc caaccggtcc ttcggcggcg cgcaggtttc ccgtactttt tacgcccgcg
     1201 ggcagaccgg ccagcagttg ctgctcggag cgtacgccgc cctgagcagg gaaattgccg
     1261 tcgggcaggt ccagatgttc ccgcgctcgg agatgctcga cctggtcgtc gccgacggcc
     1321 gcgcgcgcgg aatcgtcgtg cgcgatctgg tcaccggcga ggtgacgtgc cactcggcgg
     1381 atgccgtcct tctcgccacg ggcggctata ccaacgtcta caacctttcg accaacgccg
     1441 tcggatgcaa cgtcacggcg atctggcgcg cgcaccggcg cggggcgttc ttcgcgaacc
     1501 cgtgtttcac gcagatccac ccgacctgca ttcccgttgc cggagagcac cagtccaagc
     1561 tcaccctgat gagcgaatcc ctgcgcaacg acggtcgcat ctgggtgccc gtcaaggcgg
     1621 gcgacccgcg gcacccgggc gagattcccg aggccgagcg cgactatttt ctcgagcgcc
     1681 gatacccgac tttcgggaac ctcgtacctc gcgacgttgc ctcacgggca gccaaggcgg
     1741 tttgcgacga ggggcggggc gtcgggccgg gcaggcgtgg cgtctacctc gactttgccg
     1801 acgctttctc gcgcctgggc gaggacgtca tccgggagcg ttacgag
//
LOCUS       DASWAV010000551         2062 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U2665
            k141_632263, whole genome shotgun sequence.
ACCESSION   DASWAV010000551 DASWAV010000000
VERSION     DASWAV010000551.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960316
            Sequence Read Archive: SRR10260007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2062)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2062)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:47:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,929
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2062
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_632263"
                     /isolate="SMAG_U2665"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.36444 N 132.31889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..216
                     /locus_tag="VF958_11540"
     CDS             <1..216
                     /locus_tag="VF958_11540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005032357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF520 family protein"
                     /protein_id="HEX9149784.1"
                     /translation="VRQVAKIQQGIPSEKAKAIVKAIKDRKLKVQASIQADQVRVVGR
                     SKDDLQNAMAVVREGDFGLPLQFTNYR"
     gene            213..1223
                     /locus_tag="VF958_11545"
     CDS             213..1223
                     /locus_tag="VF958_11545"
                     /inference="COORDINATES: protein motif:HMM:NF012568.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HEX9149785.1"
                     /translation="MIFSPRSHNPRLLRAYDLISEKLALVEEELLLHFRSPIPTIDRI
                     GGHLAQGGGKRIRPALLLLCSRLLEYGEKERRDVRYATVIEFIHTATLVHDDVIDEAR
                     LRRGRTSANARWGNNLTVLFGDYLYTKSMGIALDEGDLTILKVLSDTTLSMIEGEIIG
                     TEKTGSLNLTREEALEIIRRKTADLFSAACRIPAHFAPRGDLVAAERLAEYGRCLGVA
                     FQLIDDLLDYTGSVEATGKPVLNDLREGKLTIPLLLSLPRATDAERGSVAQVMREREF
                     DSVAPTEILAIAERHGGLAETRALARDYAAAARVALGSFPESDAKDGLLLATESVIDR
                     VG"
     gene            1423..1794
                     /locus_tag="VF958_11550"
     CDS             1423..1794
                     /locus_tag="VF958_11550"
                     /inference="COORDINATES: protein motif:HMM:NF025910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX9149786.1"
                     /translation="MARHPNAIHSLATSLRQLPFFSGRRDTGSEARLYVSRALAAGAE
                     ERYDVALVFCEKALAADPANLAARLLVGRIYHHAIEDVDRAVAAYRKVITLSGYDADN
                     PYCAAAREALDALVKNFEEDA"
BASE COUNT          367 a          704 c          645 g          346 t
ORIGIN      
        1 gtccgccagg tcgcgaaaat ccagcaggga atcccctcgg agaaggcgaa ggcgatcgtc
       61 aaggcgatca aggaccgaaa gctgaaagtg caggcttcca tccaggcgga ccaggtccgc
      121 gtcgtggggc gcagcaagga cgatctccag aacgccatgg cggtcgtccg cgaaggtgat
      181 ttcggcctgc ccctgcagtt cacgaactac cgatgatctt ctcgccgcgg tcccacaacc
      241 cccggctcct gcgcgcctac gacctgattt ccgagaagct cgccctcgtc gaagaggagc
      301 tgctcctcca cttccgctcg ccgattccaa cgatcgatcg gatcggcgga cacctcgcgc
      361 aggggggcgg caagcggatc cggcccgcgc tgctcctgct gtgctcccgc ctgctcgagt
      421 acggcgaaaa ggagcggcgg gacgtccgct acgcgacggt gatcgagttc atccacacgg
      481 ccacgctcgt ccacgacgac gtcatcgacg aggcgcggct gcgtcgcggc cgcacgtcgg
      541 caaacgcgcg ctgggggaac aatctgacgg tcctcttcgg cgactacctt tacaccaagt
      601 ccatggggat cgcgctcgac gagggggacc tgacgattct gaaggtcctc tccgacacga
      661 cgctctcgat gatcgaagga gagatcatcg gcacggagaa gacgggcagc ctcaatctga
      721 cgcgggaaga ggcgctcgag atcatccggc gcaagaccgc cgacctgttc tccgcagcct
      781 gccgcatccc ggctcatttt gcgccgcgcg gagatctcgt cgccgcggag cgactcgcgg
      841 agtacgggcg atgcctcggc gtcgcgttcc agctgatcga cgacctgctc gactacacgg
      901 ggagcgtcga agccacgggc aagcccgtgt tgaacgacct tcgcgaagga aagctcacga
      961 ttccgctgct tctctcgctg ccccgcgcga ccgacgcgga gcgcgggagc gtcgcgcagg
     1021 tgatgcgcga gcgcgagttc gacagcgtcg cgcccacgga aattctcgcg atcgcggaac
     1081 gccacggtgg tctcgccgag acgcgcgcac tcgcccgcga ttacgccgcc gcggcgcgcg
     1141 tcgccctcgg gtcgttcccg gaatcggacg cgaaggacgg acttctcctc gcaaccgaat
     1201 ccgtgatcga tcgcgtagga tgatttccta actcttttct gagatttgcc tggccggaat
     1261 agatttattc ggaaagatcg ctatatcgcc tattgttatt cggatattcc cccgtggctc
     1321 ggcgacgagt cgccaccatc cgactaatgt aaggccacag atcgattttg ccgaaatttt
     1381 tctcgattcg cacgtttctg ccctatactc gttccagctc ccatggcgcg gcatccgaac
     1441 gcgattcact ctctcgcgac gagcctgcgc cagctcccct ttttctccgg gcggcgggac
     1501 accggcagcg aggcgcggtt gtacgtctcc cgggcgctgg ccgccggcgc cgaggagcgt
     1561 tacgacgtcg ccctggtctt ctgcgaaaaa gctctggcgg ccgatcctgc gaacctcgcc
     1621 gcgcgcctgc tcgtcggccg catctaccac cacgccatcg aggacgtgga ccgcgccgtc
     1681 gcggcttatc gcaaggtgat caccctctcc ggctacgacg ctgacaaccc gtactgcgcc
     1741 gcagcgcgag aggcgctcga cgcgctcgtg aagaacttcg aggaagacgc ctagagagct
     1801 cttcggagtt cgttgggccg cggcagacga ggccagcccc acggatacat cggcggcgag
     1861 ccccagacct cggcgcggta acgaccgccg gaaaatattg cctgccggga ggagtcgacg
     1921 cgccaacggc cgggggttcg aatccacggc gccgggagcg atcgcgccgc ccgcccagtc
     1981 cgccaccagc tgggtggtac gatcggcgcc gatgcgggcc agtcggaact cctggccctt
     2041 ctcgaagcgc aggctcgcgt cc
//
LOCUS       DASWAV010000552         2326 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U2665
            k141_271917, whole genome shotgun sequence.
ACCESSION   DASWAV010000552 DASWAV010000000
VERSION     DASWAV010000552.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960316
            Sequence Read Archive: SRR10260007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2326)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2326)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:47:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,929
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2326
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_271917"
                     /isolate="SMAG_U2665"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.36444 N 132.31889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>2326
                     /gene="topA"
                     /locus_tag="VF958_11555"
     CDS             <1..>2326
                     /gene="topA"
                     /locus_tag="VF958_11555"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR01051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HEX9149787.1"
                     /translation="PTYEVLHGKEKTLAELKRAAQKADVVYLAADPDREGEAISWHLK
                     ETLTPSAKDKPFRRVRFNEITKKAVLAAMDDAGEIDSRLVDAQQARRIIDRLVGYEVS
                     DLLWKKIWRGLSAGRVQTVALRIICEREKEIESFVPVEYWTVDADLEAGAPPPFTAKL
                     VSFDGQKLKFDGTNPRLESEAAASRVRGEAERAPWRVAKVDASERRKNPAPPFITSQL
                     QQAAARRLGFPVRRTMQIAQRLYEGREIPGKGTVGLITYMRTDSTRVSNDALAAVRDH
                     IFARYGTGPDVLPDSPRFFKGKRAVQDAHEAIRPTDLSLSPEEVAKHVASEEAKLYRL
                     IWERFVGSQMAPAVYDTVSAEIEAGRAVYRASGSTLKTPGYLAVYGVSAEEEDDAEKD
                     TAGTKLPPLTAGETLKLVALKPEKKATQPPPRYNEASLVKFLEENGIGRPSTYAEILR
                     KIEDREYVRKKDRRFLPTALGRTVVELLIPYFDDFFETGYTAEMEQRLDDVEEGKVSW
                     REVLAKFDEKFTKDRNRALDKMVSSKAGIPLAEARLLHLPVPVDLEEKCPQCGKKLKL
                     RMGKNGLFVACSGYPACTFTVHIPDPDEDAIDAVELESTTCEECGSPMKLRQSRAGKA
                     FLGCTAYPKCRNVVNVSIAQGKAEARPDEPTGETCPDCGSPLVRRHGRYGVYVSCSNY
                     PACRYKPPKPVLDTGVKCPKDGGAIVERRGRFRPFYGCVNYPACDFSLNVRPIPEACP
                     NCGNLYLLLRERKGGPVLTCDRAGCGFEKEPGALP"
BASE COUNT          416 a          777 c          804 g          329 t
ORIGIN      
        1 agcccacgta cgaggtcctc cacggcaagg agaaaacgct cgcggaactg aagcgtgccg
       61 cccagaaggc ggacgtcgtg tacctcgccg ccgaccccga ccgcgagggc gaggccatct
      121 cctggcacct gaaggagacg ctcacgccgt ccgcgaagga caagccgttc cggcgggtcc
      181 ggttcaacga aatcacgaag aaagccgtcc ttgcggcgat ggacgacgcg ggagaaatcg
      241 actcgcggct cgtggacgcg cagcaggcgc ggcggatcat cgaccgactc gtcggctacg
      301 aggtctccga cctcctctgg aagaagatct ggcgcggcct ctccgcggga cgagtccaga
      361 cggtcgcgct gcggatcatc tgcgagcgcg agaaggagat cgagagcttc gtcccggtcg
      421 agtactggac cgtggacgca gacctcgaag cgggcgcgcc gccgccgttc accgcgaagc
      481 tcgtctcgtt cgacggtcag aagttgaagt tcgacgggac caacccgcgc ctggaatcgg
      541 aggccgccgc gtcgcgggtg cggggggagg ccgagcgcgc gccctggcgc gtcgcgaagg
      601 ttgacgcttc cgagcgccgc aagaatccgg cgccgccgtt catcacgtcc cagctccagc
      661 aggccgcggc ccggcgtctc gggttcccgg tgcggcgtac gatgcagatc gcgcagcgcc
      721 tgtacgaggg gcgcgagatc cccggcaagg gcaccgtcgg cctgatcacc tacatgcgca
      781 cggactcgac gcgcgtgtcg aacgacgcgc tcgccgccgt gcgcgaccac atcttcgcga
      841 gatacggcac gggtcccgac gtcctgcccg attcgccgcg gttcttcaag ggaaagcgcg
      901 ccgtccagga cgcgcacgag gcgattcgcc cgaccgatct ctcgctctcc ccggaggagg
      961 tggcgaagca cgtcgcttcg gaggaggcga agctctaccg cctgatctgg gagcgtttcg
     1021 tcggatcgca gatggcgccc gccgtctacg acaccgtttc cgcggagatc gaagcgggca
     1081 gggccgtgta tcgggcatcc ggttcgacgc tgaagacgcc ggggtacctc gccgtctacg
     1141 gcgtctcggc cgaagaggaa gacgacgcgg agaaggacac cgccggcacc aaacttccgc
     1201 cgctcacggc cggggagacg ctgaaactcg tcgccctgaa acccgagaag aaggcgacgc
     1261 agcctccgcc gcgctacaac gaggcttcgc tggtgaagtt cctcgaggaa aacgggatcg
     1321 gccgcccctc gacctacgcc gagatcctgc gcaagatcga ggaccgcgag tacgtccgaa
     1381 agaaggaccg ccgtttcctc ccgaccgcgc tcggtcggac ggtggtggaa ctcctgatcc
     1441 cgtactttga cgacttcttc gagacaggct acacggcgga gatggagcag cgcctcgacg
     1501 acgtcgagga ggggaaggtc tcctggcgcg aggtcctcgc gaagttcgac gagaagttca
     1561 ccaaggaccg gaaccgcgca ctcgacaaga tggtctcgag caaggccggc atcccgctgg
     1621 cggaggcgcg tctcctccac ctgccggtgc ccgtggacct cgaggagaag tgcccgcagt
     1681 gcggcaagaa actcaagctg cggatgggga agaacggcct cttcgtcgcc tgcagcggct
     1741 atcccgcgtg cacgttcacc gtgcacatcc cggaccccga cgaggacgcg atcgacgcgg
     1801 tggagctcga gtccacgacc tgcgaggagt gcggctcgcc gatgaagctg cgccagagcc
     1861 gcgcgggcaa ggcgttcctc ggctgcaccg cgtatcccaa gtgccgcaac gtcgtgaacg
     1921 tctcgatcgc gcaggggaag gccgaggcgc ggccggacga gccgacgggg gaaacgtgcc
     1981 ccgactgcgg gagcccgctc gttcgccggc acggccggta cggggtgtac gtctcctgct
     2041 cgaactatcc ggcgtgccgg tacaagccgc cgaagccggt cctcgacacg ggggtcaagt
     2101 gtcccaagga cggcggcgcg atcgtcgaac gccgcgggag gttccggccg ttctacggct
     2161 gcgtgaacta tccggcgtgc gacttctccc tgaacgtgcg gccgatcccg gaggcttgcc
     2221 cgaactgtgg gaacctttac ctcctcctcc gggagcgcaa gggaggtccg gtcctgacgt
     2281 gcgaccgggc cggttgcggg ttcgagaagg agccgggtgc gctccc
//
LOCUS       DASWAV010000553         2796 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U2665
            k141_632615, whole genome shotgun sequence.
ACCESSION   DASWAV010000553 DASWAV010000000
VERSION     DASWAV010000553.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960316
            Sequence Read Archive: SRR10260007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2796)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2796)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:47:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,929
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2796
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_632615"
                     /isolate="SMAG_U2665"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.36444 N 132.31889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..288
                     /locus_tag="VF958_11560"
     CDS             <1..288
                     /locus_tag="VF958_11560"
                     /EC_number="6.2.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018586551.1"
                     /note="AcsA; in Sinorhizobium meliloti this enzyme is
                     required for acetoacetate activation; similar to
                     acetyl-CoA synthase; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetoacetate--CoA ligase"
                     /protein_id="HEX9149788.1"
                     /translation="GELLLFVVPREGVALDDSLSARIRRALRENLSPRHVPDEILAVA
                     SVPRTLNNKKLEVPVRRILSGEPLWKVANPGAMANPESLDYFVELAARRRR"
     gene            complement(314..685)
                     /locus_tag="VF958_11565"
     CDS             complement(314..685)
                     /locus_tag="VF958_11565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012932386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HEX9149789.1"
                     /translation="MKLVVAIVRPEKLSDVLESLFRAQVRGLTLHRVQGHGGETDRVE
                     TYRGTTVKMELSDKVRLEIGVSDHFVQPTVNAILTAARTGEVGDGKVFVLPVEKVYRI
                     RTGEEDQAAVTPIEELERTGV"
     gene            complement(682..1968)
                     /locus_tag="VF958_11570"
     CDS             complement(682..1968)
                     /locus_tag="VF958_11570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008631114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008519 - ammonium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0072488 - ammonium transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HEX9149790.1"
                     /translation="MNNSDTAWVLTATALVLLMTPALGFFYGGLVRKKNALNTMMMSV
                     CALGFGGLAWALVGYSIAFGRGNAWVGDFSMAFLRGVDLRPQGTIPHLLFFAFQGTFA
                     IITAALISGAIVERMRFGPYLAFLTLWGILVYAPVAHWVWGGGWLSRLGTLDFAGGTV
                     VHVNAAAAALTAALVLKPRKDYARQAILPHNVPFTLLGAGLLWFGWFGFNGGSALAAN
                     SIAALAFVNTMLAPIATLVVWTLLDLSRTGRATAVGAATAAVVGLVAVTPAAGFVSPI
                     SAILLGGVAAAPSYFALLWRARTRVDDSLDVVAAHGVGGTVGALLTGVLAQKSWNGAA
                     DGLLFGHPRQLAIQAAGVAATIVFSAAGTFAILKVLSALGAIRATARDEGLGLDVTQH
                     GEEAYSRGEGAILVLPEVGTTRGAPAPSLETEPGIP"
     gene            2059..2637
                     /locus_tag="VF958_11575"
     CDS             2059..2637
                     /locus_tag="VF958_11575"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX9149791.1"
                     /translation="MRYPPDQRDKSRRRILSVASRMFRESGYRATGVAAIMKAAGMTA
                     GGFYAHFPSKEALFRESLAPAFDEFRKRLTGALEGRVRSVSSSELVRRYLDVEKTAEA
                     GESCPINALAADIARSGEGVRERFEDELRKTAEAVAARTRKPGVPADDAALALLALCS
                     GAILLRRAMESPEMENRMVEACVSFAERAGGR"
     gene            complement(2689..>2796)
                     /locus_tag="VF958_11580"
     CDS             complement(2689..>2796)
                     /locus_tag="VF958_11580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018078738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sorbosone dehydrogenase family protein"
                     /protein_id="HEX9149792.1"
                     /translation="ERAWGRPVDVVVMPDGALLVSDDEAGAIYRISSTK"
BASE COUNT          495 a          965 c          916 g          420 t
ORIGIN      
        1 ggggagctgc tcctcttcgt cgttccacgc gaaggcgttg ctctcgacga ttcgctctcc
       61 gcgcggatcc gccgcgcctt gcgcgagaat ctctctccgc gccacgttcc cgacgagatc
      121 ctcgcggtcg cctcggtccc gcgcactctc aacaacaaga agctcgaagt ccccgtgagg
      181 cgcatcctct cgggtgagcc gctctggaag gtcgcgaatc ccggcgccat ggcgaacccc
      241 gagagtctcg actatttcgt ggagctcgcc gcccggcgaa ggcggtaaag ccccgttcga
      301 tcagccgcgc agatcagacc ccggtgcgct cgagctcctc gatcggcgtg acggccgcct
      361 ggtcctcttc ccccgtacgg atccggtaga ccttctcgac cggcagcacg aagactttcc
      421 cgtcgccgac ttcaccggtg cgcgcggcgg tcaggatcgc gttcaccgtg ggttggacga
      481 agtggtcgga gaccccgatc tcgaggcgca ctttgtccga cagctccatc ttcaccgtcg
      541 tgccccggta cgtctccacc cggtccgtct ccccgccgtg tccctggacc cgatggagcg
      601 tcaacccgcg tacttgcgcc cggaaaagcg actcgagcac gtcggaaagc ttttcggggc
      661 ggacgatcgc gacgacgagc ttcacggaat gccgggctcg gtctcgaggg atggcgcggg
      721 ggcacctcgc gtcgttccga cctccggcag caccaggatc gcgccctcgc cgcgcgagta
      781 cgcctcctcc ccgtgctgcg tgacgtcgag accgagccct tcatcgcggg ccgtcgcgcg
      841 gatcgcgccc agggccgaga ggaccttcaa tatcgcgaaa gttccggctg cgctgaagac
      901 gatcgtcgcg gcgacaccgg ccgcctggat cgcgagctgg cggggatgac cgaagagaag
      961 cccgtccgcc gcgccgttcc acgacttctg ggcgagcacc ccggtcagaa gcgcgccgac
     1021 cgtccctccg actccgtgag ccgcaaccac gtcgagcgaa tcgtccaccc gtgtccgtgc
     1081 ccgccagagg agcgcgaagt agctcggcgc cgccgcgact cccccgagaa ggatcgccga
     1141 gatcggactg acgaatcccg ccgccggcgt gaccgccacg aggccgacaa cggctgcagt
     1201 cgccgcgcca acggccgtcg cgcggccggt gcgcgagagg tcgagcagcg tccagacgac
     1261 gagcgtcgcg atcggcgcga gcatcgtgtt cacgaacgca agcgcggcga ttgaattcgc
     1321 cgcgagagcg ctccccccat tgaatccgaa ccagccgaac cacagcagcc cggcgccgag
     1381 cagcgtgaag gggacgttgt gcgggaggat ggcctgccgc gcgtagtcct tgcggggctt
     1441 gagcacgagc gccgcggtca gcgccgccgc ggccgcgttg acgtggacga cggtgccgcc
     1501 ggcgaagtcg agggtgccga gtctcgagag ccagccaccg ccccagaccc agtgcgccac
     1561 cggcgcgtac acgaggatcc cccacaaagt caggaacgcg aggtacggac cgaagcgcat
     1621 ccgctcgacg atcgctccgg agatgagcgc cgccgtgatg atcgcgaagg tcccctgaaa
     1681 cgcgaaaaag agcaggtgcg ggatcgttcc ctggggacgg aggtccaccc ccctcaggaa
     1741 cgccatcgag aagtcaccca cccaggcgtt tcctcgcccg aacgcgatcg aatagcccac
     1801 gagcgcccac gcgagtcctc cgaaaccgag tgcgcagacg ctcatcatca tcgtgttcag
     1861 agcgttcttc ttgcgaacga gaccgccgta gaagaaaccg agggcgggag tcatgaggag
     1921 aaccagggcc gtcgccgtga ggacccaggc ggtatccgaa ttgttcaact tgggaaatca
     1981 gcctaacaga ctggcctcga cctgtcacgc ggaaaatgca cgtttgacat cagattacaa
     2041 tcatcatatt acttcgacgt gcgctatcca ccggatcaga gggacaagtc gcgccggcgc
     2101 atcctttcgg tggcgagccg gatgttccga gagagcggct atcgcgcgac cggggtcgct
     2161 gccatcatga aggcggccgg gatgactgcc ggcggcttct acgcccactt tccgtccaag
     2221 gaggcactct tccgggagag cctggctccg gcattcgacg aatttcgcaa gcgcctgacg
     2281 ggtgccctcg agggacgagt aaggtcggtc tcctcgagcg agctcgttcg gcggtatctc
     2341 gacgtggaga agaccgcgga ggccggagag agttgcccga tcaacgctct cgcggcggac
     2401 atcgcgcggt cgggggaagg ggttcgcgaa aggttcgaag acgagcttcg gaaaacggca
     2461 gaagccgtcg ccgcccgcac gcggaagccc ggcgtcccgg ccgacgacgc ggcgctcgcg
     2521 ctcctcgcgc tttgttcggg cgcaatcctg cttcgtcgcg caatggagag cccggaaatg
     2581 gagaaccgca tggtcgaagc ctgcgtgagt ttcgccgagc gggcgggcgg ccgctgacgg
     2641 gcgtttccga gcgggccatc ggctcggggg ggaggccccc gaccggtctc acttcgtgga
     2701 gctgatccgg tagatcgcgc cggcctcgtc gtcggacacg aggagcgccc cgtccggcat
     2761 cacgaccaca tccaccggac gaccccacgc acgctc
//
LOCUS       DASWAV010000554         4780 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U2665
            k141_512056, whole genome shotgun sequence.
ACCESSION   DASWAV010000554 DASWAV010000000
VERSION     DASWAV010000554.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960316
            Sequence Read Archive: SRR10260007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 4780)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4780)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:47:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,929
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4780
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_512056"
                     /isolate="SMAG_U2665"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.36444 N 132.31889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..415
                     /locus_tag="VF958_11585"
     CDS             <1..415
                     /locus_tag="VF958_11585"
                     /inference="COORDINATES: protein motif:HMM:NF019118.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="HPr kinase/phosphorylase"
                     /protein_id="HEX9149793.1"
                     /translation="VEIRKYPSGAVIGRAADMLRYHMELRGIGIINIKHLFGVSAVRA
                     SKSIDLVIELERWDPTKTYDRLGLDGETYAILDRARPYLKLPVASGRNIALLIEIAAR
                     NELLKTQGYDAAKEFALKVDEEIARNALSPGLKRA"
     gene            466..1383
                     /gene="rapZ"
                     /locus_tag="VF958_11590"
     CDS             466..1383
                     /gene="rapZ"
                     /locus_tag="VF958_11590"
                     /inference="COORDINATES: protein motif:HMM:NF015620.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HEX9149794.1"
                     /translation="MFAARRTAARKPSRSTAAGEARPITESLVVVTGLSGSGKSYVFK
                     CLEDMGYFCVDNLPLPLVEPLLSHVTAPRVGVILDVRNPDFAARFPPILSRLRARVPG
                     TRLLFLDASEESLIRRFSETRRPHPLSVDRPLLTALRNERELLEDVRTLADVVVDTSA
                     MTVHELRSFIQKTFVGEETAARMVVSATSFGYKFGVPHDVDLIFDVRFLANPHFVPEL
                     KPKVGTDPAVAAYIEKDPETAEFLERLAGFLDYLLPLYEREHKSYLSIGIGCTGGRHR
                     SVYVAEQLARMLQARGLPVRVSHRDATRE"
     gene            1380..1892
                     /locus_tag="VF958_11595"
     CDS             1380..1892
                     /locus_tag="VF958_11595"
                     /inference="COORDINATES: protein motif:HMM:NF015567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9149795.1"
                     /translation="MMKRLATGERIPHRDRPEQQPVIGILLVTHGRLAEELKTAALTI
                     QPDIEQIVAVALDWSQVGDDASRQIAEGLEKADRGDGAVILTDMFGGTPTNLTLPFLK
                     RDRVEIVTGVNLPMLLKCAALQKSGKPVVEIAHVAKDRGQRSIYVASDLLADEAVAAA
                     GLAPDKGEGE"
     gene            1889..2158
                     /locus_tag="VF958_11600"
     CDS             1889..2158
                     /locus_tag="VF958_11600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013219370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HPr family phosphocarrier protein"
                     /protein_id="HEX9149796.1"
                     /translation="MIERELAIRNRLGLHARAAAKFVQTASRYKSEVKIRKNGEEVDG
                     KSILGLLLLAASQGTQITIAVSGEDENAAFEAVQGLVERRFDEDE"
     gene            2192..3880
                     /gene="ptsP"
                     /locus_tag="VF958_11605"
     CDS             2192..3880
                     /gene="ptsP"
                     /locus_tag="VF958_11605"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008965 - phosphoenolpyruvate-protein
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0006808 - regulation of nitrogen
                     utilization [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HEX9149797.1"
                     /translation="MLQGLAVSPGIAVGRAVIVRFGGLPAFRRAVSPDEFAEEERRLR
                     RASRLTAEEFRRHSGEAAGDIGEELAAIIEAHGLIASDETYLQAILDRMRRDRVNVEW
                     ALAEVTRELGFRFERADSDVMRERAADIHDVGREIARQLTGGAPLLPEEMPRGSILIA
                     DEISPVEVSRLDPRRVRALALERGGPTSHATIIARSFGLPAVVGIEGLCDAISPETPI
                     VVDGDRGTVDANPSKDRLRRSLLRVREAREKARRLRARVPRTAATKDGVRIAVRANVE
                     LAEEIFALERYHAEGVGLFRSEFLYLRAATGAPGVEEQRAVYEDLLAAAAPHPVVVRT
                     YDLGGEKGIGPVRGENPALGLRGLRYCLAHPELFDAQLTALCLASRTGPLSVMLPMVT
                     SLWELQEARIALGAAAQRTGLAEPPPLGVMIEVPSAALLADRLAAAADFFSIGTNDLA
                     QYALAVDRANPDVASLYRPLHPAILRMVRSVVEAGRANNRPVAVCGELAAEPIGIAVL
                     IGLGIVDFSVTPVAIAGVKDSLATLDSARARALVEKALSAAEASEVEKLFRGRV"
     gene            3877..4233
                     /locus_tag="VF958_11610"
     CDS             3877..4233
                     /locus_tag="VF958_11610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014345441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEX9149798.1"
                     /translation="MRRVPKPWGHELIFAENNRYAGKILHIEPGHRLSLQYHERKDET
                     FYVLNGEVNLTIEQDGRLREIRLQQGSAYRIPPGVRHRMRAEAGPCDLIEVSSPELDD
                     VVRLEDAYGRVGTTAP"
     gene            4250..>4780
                     /locus_tag="VF958_11615"
     CDS             4250..>4780
                     /locus_tag="VF958_11615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9149799.1"
                     /translation="MTTPLRLRRLSIVVAAALSALAAAPRPTATPTPGPRLTGGFGKT
                     PSAPSSQRSGEKKKVRITNDSLVTEPDKGKLTTSEVRRAPTPTPGAGAGEGPAASGAP
                     AASSEPAEGGEEYWRGEARRLRDRVGGLKETIARLENETKKLEGDFYSWDDGAYRDRV
                     IKPAWDKAREELATARK"
BASE COUNT          813 a         1629 c         1605 g          733 t
ORIGIN      
        1 cgtcgagatc cggaagtatc cgagcggcgc ggtcatcggc cgcgccgcgg acatgctccg
       61 ctaccacatg gagcttcgcg gaatcggaat catcaacatc aaacacctct tcggagtctc
      121 ggcggttcgc gcctcgaagt cgatcgacct cgtcatcgag ctcgaacggt gggaccccac
      181 gaagacctac gaccgcctcg gcctcgacgg cgagacctac gcgatcctcg accgcgcgcg
      241 accctacctc aagcttcccg tcgcctcggg ccgcaacatt gccctcctga tcgagatcgc
      301 ggcgcgcaac gagctcctga agacgcaggg ctacgacgcg gcgaaggagt tcgcgctcaa
      361 ggtggacgag gagatcgcgc gcaacgccct ttcgcccggg ttgaaacgcg cttgagcgcc
      421 cgacggcgcc ctcccggtcg cgccaccgcc cgcctcacga aaaaagtgtt cgcggcccgg
      481 cggacggccg cgaggaagcc ctctcgttcc acagccgccg gggaagcccg gccgatcacc
      541 gagagtctcg tcgtcgttac cggcctgtcc ggctcgggga agagctacgt cttcaagtgt
      601 ctcgaggaca tgggctactt ctgcgtggac aacctcccgt tgccgctggt cgagccgctg
      661 ctctcgcatg tgacggctcc gagggtgggc gtgatcctcg acgtccgcaa cccggacttc
      721 gcggcgcgct tccccccgat cctctcccgc ctccgcgcgc gcgttcccgg cacgcgactc
      781 ctcttcctcg acgcctccga ggagtcgctc atccggcgct tctccgagac gcgccgcccg
      841 cacccgctct ccgtcgatcg accgctcctg acggcgctcc gcaacgagcg cgagttgctc
      901 gaagacgtgc gaacgctcgc ggacgtagtc gtcgacacgt cggcgatgac cgtgcacgag
      961 ctgcggtcct tcatccagaa gaccttcgtc ggcgaggaga ccgccgcgcg aatggtggtg
     1021 tcggcgacgt cgttcggcta caagttcggc gttccccacg acgtcgatct catcttcgac
     1081 gtccgattcc tcgcgaaccc gcacttcgtt ccggagttga agccgaaggt gggcaccgac
     1141 ccggcggtgg ccgcctacat cgaaaaggat ccggagaccg ccgaattcct cgagagactc
     1201 gccggattcc tcgattacct gctgcccctc tacgagcgcg agcacaagag ctacctgtcg
     1261 atcggaatcg gctgcacggg ggggcggcac cggtcagtct acgttgccga acagctcgcg
     1321 agaatgctcc aggcgagagg tctcccggtg cgagtgtcgc atcgcgatgc gacgcgggaa
     1381 tgatgaaacg cctggcgacc ggtgaaagaa ttccgcatcg tgatcgtccg gagcagcagc
     1441 cagtgatcgg gatcctcctg gtcacgcacg ggcgtctcgc cgaagagctc aagacggccg
     1501 cgctgacgat ccagcccgac atcgagcaga tcgtggcggt cgcgctcgac tggagccagg
     1561 tcggagacga cgcgagccgc cagatcgccg aaggtctcga aaaggcggac cgcggcgacg
     1621 gcgcggtgat cctcacggac atgttcggag gcacaccgac gaacctgacg cttccgtttc
     1681 tgaagcgcga ccgcgtagag atcgtgaccg gagtcaatct gccgatgctc ctgaaatgcg
     1741 ccgccctcca gaagagcggc aagccggtgg tcgagattgc gcacgtcgcg aaagatcgcg
     1801 gacagcggtc gatctacgtc gcctcggacc tcctcgcgga cgaggccgtc gccgccgcag
     1861 ggcttgcgcc ggacaagggg gagggggagt gatcgagcgg gagctggcga tccggaaccg
     1921 cctcgggctc cacgcccggg ccgccgcgaa gttcgtccag acggcgagcc gctacaagtc
     1981 cgaggtcaag attcgcaaga acggcgaaga ggtcgatggc aagagcattc tcgggctcct
     2041 cctcctcgcc gcctcgcagg gaacccagat caccatcgcc gtgagcggcg aagacgaaaa
     2101 cgccgccttc gaggcggtgc agggattggt cgagcgccga ttcgacgagg acgagtaatc
     2161 gttccgccga aagaaacggg ttccgaaacc aatgctccag ggcctggccg tttcccccgg
     2221 catcgcggtg ggtcgcgccg tcatcgtgcg cttcgggggg ctgcccgcct tccgtcgcgc
     2281 cgtgtctccc gacgagttcg ccgaggagga gagacggctt cggcgggcgt cgcggctcac
     2341 tgcggaagag ttccggcgtc actcgggcga ggcagcgggt gacatcggcg aggagctcgc
     2401 ggcgatcatc gaggctcacg gcttgatcgc ctccgatgag acctacctcc aggcgatcct
     2461 cgatcgaatg cggcgcgacc gcgtcaacgt cgagtgggcc ctcgccgagg tgacgcggga
     2521 gctcggtttc cgtttcgaga gagcggattc cgacgtgatg cgcgagcgcg cggcggacat
     2581 tcacgacgtg ggacgggaaa tcgcgcgaca gctcaccgga ggcgcgccgc tcctccccga
     2641 ggagatgccg cgcggctcga tccttatcgc cgacgagatt tcaccggtgg aggtctcccg
     2701 cctcgacccg cggcgcgtcc gcgcactcgc tctcgagcga ggcggtccga cctcgcacgc
     2761 aacgatcatt gcgagatcgt tcggcctgcc ggccgtcgtc gggatcgagg gcctctgcga
     2821 cgcgatctct ccggagacgc ccatcgtggt cgacggggac cggggcaccg tcgatgcgaa
     2881 cccttcgaag gaccggcttc gaaggagcct gctgcgcgtg cgagaggcgc gcgagaaggc
     2941 caggcgcctg cgcgcccggg tgccgcgcac ggcagcgacg aaggacggag tgaggatcgc
     3001 cgtgcgtgcg aacgtggagc ttgccgagga gatcttcgcg ctcgagcgct accacgccga
     3061 aggcgtcggc ctcttccgct cggagttcct ctatctgcgg gcggcgacgg gcgcgccggg
     3121 cgtcgaagag cagcgcgcag tctacgaaga tctgctggcc gccgctgcgc cgcacccggt
     3181 ggtcgtgcgg acgtacgacc tcggaggcga gaaggggatc ggtccagtgc gcggcgagaa
     3241 cccggctctc ggcctgcgcg ggttgcgcta ctgcctcgcc caccccgagc tcttcgacgc
     3301 gcaactgacg gcgctgtgtc tcgcgtcccg aacgggaccg ctctccgtca tgctgccgat
     3361 ggtgacgtcg ctctgggagc tgcaggaggc gcgaatcgcg ctcggggccg ccgcacagcg
     3421 gacgggtctc gccgagcccc caccgctcgg cgtgatgatc gaagttccgt cggcagcact
     3481 gctcgccgac cgcctcgcgg cggcagcgga tttcttttcg atcgggacca acgacctcgc
     3541 gcaatacgcg ctcgccgtcg accgcgcgaa tcctgacgtc gcgtcccttt accgtccgct
     3601 ccatccggcg atcctccgga tggtacgatc cgtcgtcgaa gccggccgcg cgaacaacag
     3661 gccggtagcg gtgtgcggcg agctggccgc cgaaccgatc gggatcgccg tcttgatcgg
     3721 cctcggcatc gtcgatttct ccgtcacgcc ggtcgcgatt gcgggggtga aggattccct
     3781 cgcgaccctc gactctgcgc gggctcgggc tctggtcgag aaagctctct cggcagccga
     3841 ggcttcggag gtggaaaaat tgtttcgagg acgagtgtga ggagggtacc gaagccctgg
     3901 gggcacgagt tgatcttcgc ggaaaacaac cggtatgcgg gcaagatcct ccacatcgag
     3961 ccgggacacc gtctctccct ccagtaccat gaaagaaagg acgagacctt ctacgttctc
     4021 aacggggagg tgaacctcac gatcgagcag gatgggcggt tgcgggagat ccggttgcag
     4081 caggggagcg cgtatcggat ccctcccgga gtgcgtcacc ggatgcgggc cgaagccgga
     4141 ccctgcgacc tgatcgaggt gtcttccccc gagctcgacg acgtcgttcg actcgaggac
     4201 gcctacggac gtgtgggcac gaccgcgcca taatcatcct ctcgggtcca tgacgacacc
     4261 cctccgcctc cggcgccttt cgatcgtcgt cgcggccgcg ctctccgcgc tcgccgccgc
     4321 gccgagaccg acggccacgc cgacccccgg gcctcggctg accggtgggt tcggcaagac
     4381 gccttccgcc ccctcctcgc agcgatccgg cgagaagaaa aaagtgcgca tcacgaacga
     4441 ttcgctcgtc accgaaccgg acaaggggaa actgacgacc tccgaggtcc gccgagcgcc
     4501 gaccccgact cccggagccg gggccggcga ggggcctgcg gcgtcgggag cgcccgccgc
     4561 gtcatcggag ccggccgaag gcggtgagga gtactggcgc ggcgaagccc ggcggcttcg
     4621 cgatcgcgtg ggcggcctca aagagaccat cgcccgcctc gaaaacgaga cgaagaagct
     4681 cgaaggcgac ttctactcgt gggacgacgg cgcctaccgc gatcgggtga tcaagccggc
     4741 ctgggacaag gcgcgcgaag agctcgcgac cgcgcggaag
//
LOCUS       DASWAV010000555         3679 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U2665
            k141_231625, whole genome shotgun sequence.
ACCESSION   DASWAV010000555 DASWAV010000000
VERSION     DASWAV010000555.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960316
            Sequence Read Archive: SRR10260007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 3679)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3679)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:47:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,929
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3679
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_231625"
                     /isolate="SMAG_U2665"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.36444 N 132.31889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            168..2741
                     /locus_tag="VF958_11620"
     CDS             168..2741
                     /locus_tag="VF958_11620"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HEX9149800.1"
                     /translation="MSIAPGTRLGPYEVLGPLGAGGMGEVYRASDTRLKREVAVKILP
                     ASFSADGDRLRRFELEAETAGRLNHPNILAIYDIGTHDGAPYVVSELLEGETLRDRLS
                     GGALAPRRAIDYARQLAAGLAAAHEKGIVHRDLKPENVFVTSDERIKILDFGLAKLTQ
                     PDVSSGGGTNLPTSPGTEPGVVLGTAGYMSPEQVRGAAVDHRSDVFSFGAILYEMLMG
                     SRAFRGDSAIETMNAILKEDPAEPSQSGRTVPPVLERIVRHCLEKNPAQRFQSVRDLA
                     FDLESLSDTSSPATAIVQPSARPRSRALPILAGLVLVALAAGAGYFAGAGGNGPKAPT
                     YQRLTFRNGSVFLARFAPDGQTIVYSGAWEGDPFQVFSSRPGSAESRSLGLPPAYIDS
                     ISGDGEMAVVLNPSGAFGAGTLAQVPLAGGAPRPILDSVRLASWAPDGKTLAVTRIVG
                     GRQRVEYPVGKTLYETAGFVNDLRVSLDGNRVAIEETLARTISGGTGWVGFLDRAGRR
                     TTLYTGWIRNCGLAWSPDGREVWFAVPDRSGAFRDVLASSRPGATRHLVRMAGAMTIH
                     DVSRDGRLLFTRENVRLHALVGAEGEKEERALSWLDESLVADLSPDGRTLLFSETGGG
                     GGSHGAVYVRATSGGEAVRLGEGFGLALSPDGKWALTMDASTPPRLILLPTGVGQPKP
                     LAVAGFESYYFARWLPDGRRIFFDANPPGRKIQCHILDLEGGSPRPVGPEGGSCWSAS
                     PDGKLLALTASDGSLALYPVDGGGPARPIPGWTPGATPIQWSADGGSLYFAAETEIPA
                     RIQRFDLATGQSQPWRQLAPSDRAGVQAVNNPLVTPDGRTYAYTYVRLLGDLYLAEGL
                     K"
     gene            2827..>3679
                     /locus_tag="VF958_11625"
     CDS             2827..>3679
                     /locus_tag="VF958_11625"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEX9149801.1"
                     /translation="MTLSAGTKLGPYEVLGPLGAGGMGEVYRARDTKLDRDVAIKVLP
                     ERLASDPTALGRFEREAKTVAALSHPNLLGIYDFGTHEGVSYAVTELLSGETLRDRLA
                     AGPIPRRKAVEYALQIARGLGAAHERGIAHRDLKPENVFVTTDGRVKILDFGLAKPLL
                     PESGVSGAATFGATEPGTVLGTVGYMSPEQVRGKPADHRSDIFAFGAILYEMLSGQRA
                     FKGDSAVETMNAILIHDLPEPSGPADDTLPPLLDRIVRHCLEKNPEERFQSVRDIAFD
                     LEALSGST"
BASE COUNT          556 a         1291 c         1238 g          594 t
ORIGIN      
        1 cttgtcgcgc cgccggatcg cgtcgaggtt cttctcgaag actgcccggg cggccgagac
       61 gtcggagccc gcgggttggg ccgacaacat cgcgctcagg atggagggca gcagccacgt
      121 cattttcgct cctccgattt cccggaagga tatccttcgc ggaaacagtg agcatcgcgc
      181 ccggaacgcg cctgggtccc tacgaagtcc tcggtccgct cggggcggga ggcatgggcg
      241 aggtctatcg ggcgagcgac acgcgcctga agcgcgaagt cgcggtcaag atccttccgg
      301 cctcgttctc cgcggacggc gaccgcctgc ggcgcttcga gctcgaggcg gagaccgccg
      361 gacgcctcaa ccaccccaac atcctcgcga tctacgacat cgggacccac gacggcgccc
      421 cctacgtcgt ctcggagctt ctcgaggggg agaccctgcg cgatcgcctt tccgggggcg
      481 cgctcgctcc gcgccgcgcg atcgattacg cgcgccagct cgcggcgggc ctcgcagccg
      541 cgcacgagaa agggatcgtc catcgcgact tgaagcccga gaacgtgttc gtgacgtccg
      601 acgagcggat caagatcctc gacttcggtc tcgcgaagtt gacgcagccg gacgtatcgt
      661 ccggcggcgg gacgaacctg ccgacctcgc cggggaccga gccgggtgtc gtcctcggca
      721 ccgcgggcta tatgtctccc gagcaggtcc gcggcgcggc cgtcgaccat cgctctgacg
      781 tcttctcctt cggcgccatc ctctacgaga tgctgatggg atcgcgcgca ttccggggcg
      841 actccgcgat cgagaccatg aacgcgatcc tcaaggaaga cccggcggag ccgtcgcagt
      901 cggggcgaac cgtgccgccc gtcctcgagc ggatcgtgcg gcactgtctc gagaagaacc
      961 cagcccagcg gtttcaatcc gttcgcgacc tcgcgttcga cctcgaaagt ctttcggaca
     1021 cgtcttcgcc ggcgacggcg atcgtgcagc cttccgcgcg cccgcggagt cgtgctcttc
     1081 cgatcctcgc cggactagtc ctcgtcgcgc tggccgccgg cgccggttac ttcgcgggag
     1141 ccggcggtaa cggaccgaag gctcccacct atcagaggct cacgttccgc aacggctccg
     1201 tcttcctcgc gcggttcgcg cccgacgggc agaccatcgt gtacagcggc gcgtgggagg
     1261 gggacccttt ccaggtgttc tcgagccggc cgggcagcgc cgagtcgcgc tctctcggcc
     1321 ttccccccgc ctacatcgac tcgatctcgg gagacggaga gatggcggtc gttctcaatc
     1381 cgagcggcgc gttcggcgcc ggtactctcg cacaggtccc gctcgccggc ggcgcccccc
     1441 gtccgatcct cgacagcgtc cggctcgcct cctgggctcc cgacgggaag accctcgccg
     1501 tcacccgaat cgtcggcgga cggcagcggg tcgaataccc ggtcggcaag accctctacg
     1561 agaccgcggg gttcgtgaac gacctgcgcg tttccctcga cggaaaccgg gtggccatcg
     1621 aagagacgct ggcccggacg atatctggcg gaacgggctg ggtcggcttt ctcgaccgcg
     1681 cgggtaggcg gacaacgctc tacaccggct ggattcggaa ctgcggcctc gcgtggtcgc
     1741 ccgacggcag agaggtctgg ttcgccgtgc ccgaccgctc cggtgctttc cgggacgtgc
     1801 tggcgagctc gcgccctggc gccacccggc acctcgtgcg gatggcggga gccatgacga
     1861 tccacgatgt ctctcgcgac ggccgactgc tcttcacgcg ggaaaacgtg cggttgcatg
     1921 cgctcgtcgg cgccgaaggc gagaaagagg aacgtgcgct gtcctggctc gacgagtcgc
     1981 tggtcgccga cctctcgccg gacggccgga cgctgctctt cagcgaaacg gggggaggcg
     2041 gagggtcgca cggcgcggtc tacgtccgtg cgacgagcgg gggcgaagcg gttcgcctcg
     2101 gggagggatt cgggctcgcc ctttccccgg acgggaagtg ggcgttgacg atggatgcga
     2161 gcacgccgcc gcgtctgatc ctcctgccaa ccggagtcgg gcagccgaag ccgctcgcag
     2221 tcgcgggctt cgagagctac tacttcgcgc gctggctacc cgacgggagg cgcatcttct
     2281 tcgacgcgaa tccgccgggc cgtaagatcc agtgtcacat cctcgatctc gaggggggat
     2341 cgccgagacc ggtggggccg gagggcggtt cgtgctggag cgcgtctccc gacggaaagc
     2401 tgctcgcatt gaccgcatcc gacgggagcc tggcgctcta tccggtcgat ggcggcgggc
     2461 ccgcgcgtcc gatcccgggt tggacgcccg gcgcgacgcc catccagtgg agcgcggacg
     2521 gcggctctct ctatttcgcg gcggagacgg aaatccccgc ccggatccag cggttcgatc
     2581 tggcgaccgg tcagtcgcag ccgtggcgcc agctcgcgcc gtccgatcgc gcgggcgtac
     2641 aggcggtcaa caaccctctc gtcactcccg atgggcgcac gtacgcctat acgtacgtcc
     2701 gtctcctcgg ggacctctat ctcgctgagg gcctgaagtg accgggggtc aggtcttcac
     2761 ttgccacatt gggggtcagg tctccacttg ccacattggt gatagagagg acctggactt
     2821 cctccaatga cactttccgc cgggacgaag ctcggtccct acgaggtcct ggggcctctc
     2881 ggcgcgggcg gcatgggaga ggtctaccgc gcgcgcgaca cgaagctcga ccgcgacgtc
     2941 gccatcaagg tccttcccga gcgcctggcc tccgacccga cggcgctcgg ccgcttcgaa
     3001 cgcgaggcga agaccgtcgc ggcgctctcg cacccgaatc tcctcggcat ctacgacttc
     3061 ggcacgcatg aaggcgtctc gtacgccgtc acggagctcc tctcgggaga gacgctccgc
     3121 gaccggctgg cggcgggacc gatcccgagg cgcaaggcgg tcgagtacgc gctccagatc
     3181 gcgcgcgggc tcggcgcggc gcacgagcgc ggcatcgcgc accgggactt gaagcccgag
     3241 aacgtcttcg tcaccaccga cggacgcgtg aagatcctcg acttcgggct cgcaaaaccg
     3301 ctgcttccgg aatccggtgt atccggcgcc gcgaccttcg gcgcgaccga gccgggcacc
     3361 gtgctcggaa cggtcggcta catgtcgccc gagcaggtgc gcggcaagcc cgccgaccat
     3421 cgcagcgaca tcttcgcttt cggcgcgatc ctctacgaga tgttgtcggg ccagcgcgcg
     3481 ttcaagggcg actcggccgt cgaaacgatg aacgcgatcc tgatccacga tctcccggag
     3541 ccgagcggcc cggcggacga cacgctcccg ccgctcctcg atcggatcgt gcgccactgc
     3601 ctcgagaaga atcccgagga gcgcttccag tcggttcgcg acatcgcgtt cgacctcgag
     3661 gcactctccg gctcgactt
//
LOCUS       DASWAV010000556         3156 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U2665
            k141_670471, whole genome shotgun sequence.
ACCESSION   DASWAV010000556 DASWAV010000000
VERSION     DASWAV010000556.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960316
            Sequence Read Archive: SRR10260007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 3156)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3156)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:47:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,929
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3156
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_670471"
                     /isolate="SMAG_U2665"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.36444 N 132.31889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(197..703)
                     /locus_tag="VF958_11630"
     CDS             complement(197..703)
                     /locus_tag="VF958_11630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9149802.1"
                     /translation="MQETKDLLRHFLGAIAYRTQKAVRGCSSDFPSFRAGAGIRSPHE
                     LVHHMDSVLGYARTFFVGGTYRVPLLPTFEAEVARFHETLAELSRVVAEKTEWNRISP
                     KQLLQGPLCDAMTHAGQLALLRRLSGDPVPPENFVFAAVSSERLGPDQALPVSPDTEW
                     PERPKSER"
     gene            complement(780..1703)
                     /locus_tag="VF958_11635"
     CDS             complement(780..1703)
                     /locus_tag="VF958_11635"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014983273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase family protein"
                     /protein_id="HEX9149803.1"
                     /translation="MNILDAIGNTSLVRLRKVVPPDCAQILVKLEWENPTGSVKDRMA
                     KAVISRAEEDGRLKPGGTVVEYTGGSTGTSLALVCAVKGYRARIVSSDAFSQEKRDHM
                     AALGAELTLVPSEGGRTTRKLILDMIEVARGLSREPHTYWTDQLNNHDSITGYYPLGE
                     EIWSQTNGEIDAFVHCAGTAATSRGVATVLRRYNPSVRIVVVEPAESAVLGGGQAGPH
                     GIEGVGIGYTPPLWEPSLVDEVLPVGTDEAKEMTRRIAREEALFAGTSSGANVVAAIR
                     VAKRLGPDARVVTLMADSGLKYLSTDVYRRR"
     gene            complement(1800..>3156)
                     /locus_tag="VF958_11640"
     CDS             complement(1800..>3156)
                     /locus_tag="VF958_11640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9149804.1"
                     /translation="GELAISLNRHFMFGFETVGTLARVPLAGGAPREILENVEDADWS
                     PDGKSLAVARHVGNRNRLDYPIGKVLYDAPGWVSDVRVSPDGRLVAFIDHPLRGDNVG
                     NVKVVDTDGKVRLTGPFAISGLAWSPRGNEVWSSGRDSISATLLSGKTRMIWNVPAGF
                     LRDVARDGRVLCAVNSSRREIVGVSAADKVERNLTWLNWSFPKGISPDGKTVLFEEQN
                     IEPQGVYLRKLDGSPAVRIGDGGAWGFSPDGRWALTIRREGESSQLALLPTGPGEPKT
                     LPKTDIRAQAATWFPDGRRILFSGNEPGHGSRLFVQDIPDGKPRPITPEGVGIRFDVV
                     SPDGKWAVATGTDRRIALYPIEPGEPRVVPGTEPDDIPLKWTTDGGSIFVYRPSAPPL
                     RIEKVDVKTGRRTLWKEIRPPDPSGVEQVGPAQITPDETSYVYSYRRALDDLYLATGL
                     R"
BASE COUNT          535 a         1047 c         1012 g          562 t
ORIGIN      
        1 gcgatcgcaa tggccacgaa gggcaccagc gacagcgcga ggaagaaggc cacctctgca
       61 gcagcgctat cgagcggacc gagcctcgcc atcgccgttc ttggcttggc ccaatccatg
      121 gtggacggac ttatagcacg cccgtcggcg aggaaacgcc gctatccgga gggcttcaat
      181 ggcgattctg gccgcttcac ctctcggact tcgggcgctc tggccactca gtatccggag
      241 acacgggaag cgcctgatcc ggaccgaggc gctccgaact gaccgcggca aacacgaagt
      301 tttccggtgg caccgggtct cccgacaggc gacgcaggag cgccagctgc ccagcgtgcg
      361 tcatggcatc acacaatggc ccctgcagaa gctgcttcgg ggaaatccgg ttccattccg
      421 tcttttccgc cacgactcgc gagagctcgg ccagcgtttc gtgaaagcgc gccacctctg
      481 cctcgaaggt cgggagcaac ggaacacggt aggttcctcc gacaaaaaac gttcgcgcgt
      541 agccgaggac gctatccatg tggtggacca gttcgtgcgg cgaccgtatc ccggcaccag
      601 cacgaaagct cgggaagtcg gacgagcagc cgcggacagc tttctgggtt cgataggcaa
      661 tggcgccgag gaagtgccgg agaaggtcct tcgtctcttg catcgttcga tcctaccgag
      721 gcgtttggcc gcgagaccga gcgttatcgg ttcggtcccg tgctgatcga ggccgtctct
      781 tatcttctgc ggtacacgtc ggtactcaag tacttgaggc cggagtcggc catcagggtg
      841 accaccctgg catcgggacc aagtcgcttt gcgactcgaa tcgcagcgac gacgttcgcc
      901 ccggaggacg tccccgcgaa gagggcttcc tcccgcgcga tgcgcctcgt catctcttta
      961 gcctcgtcgg ttccgaccgg caggacttcg tcgacgagac tcggttccca gagcggagga
     1021 gtgtagccga tcccgactcc ttcgatcccg tgagggcccg cctggcctcc ccccaacacc
     1081 gcggattccg cgggttcgac gacaacgatc ctgacactcg ggttgtatct cctcaaaacg
     1141 gtggcgaccc cgcgcgaagt ggctgccgta cccgcgcagt gaacaaaggc gtcgatctcg
     1201 ccattcgtct ggctccagat ttcctcaccc aacgggtagt agcccgtgat gctgtcgtgg
     1261 ttgttgagct ggtcggtcca atatgtatga ggctctcggc tcaacccccg ggcgacctcg
     1321 atcatgtcca ggatcagctt cctggtggtg cggcctccct cgctggggac gagcgtcaac
     1381 tcggcaccca gcgccgccat gtgatcccgt ttctcctggc tgaacgcatc ggagctgacg
     1441 atccgcgcac ggtagccctt gacggcgcag accagcgcga gcgatgtgcc cgtgctgcca
     1501 ccggtgtact cgacgacggt gcctccgggc ttcagtcgac cgtcctcctc cgcccgcgag
     1561 atcacggcct tcgccatgcg atccttgacg ctgccggtgg ggttctccca ctcgagcttc
     1621 acgaggatct gcgcgcaatc gggtggaacg accttgcgga gtcgaacgag cgatgtgttt
     1681 ccgatggcgt cgagaatgtt catggttgag ctctctttgc ttcagccgtc caaggcccgc
     1741 gagcgccgga ccctcgtcct ccgcatgact cgagatcggc aagcgcgcgg cggcgcgatt
     1801 cagcgcaacc cggtcgccag atagagatcg tcgagagctc gacggtacga gtacacgtaa
     1861 gacgtttcgt ccggcgtgat ctgcgccggg ccgacctgtt cgacgccgga cgggtcgggc
     1921 ggccggatct ctttccagag cgtgcgccgc cccgtcttca cgtcgacctt ctcgatgcgc
     1981 agaggaggcg ccgacggtcg gtagacgaag atcgagccgc cgtcggtcgt ccacttgagc
     2041 gggatgtcgt cgggctcggt tccgggcacg acgcgaggtt cgcccggttc gatcggatag
     2101 agggcgatcc ggcggtccgt tcccgtcgcc acggcccact tcccgtcggg ggacacgacg
     2161 tcgaagcgaa tgccgactcc ttcgggagtg atggggcggg gcttcccgtc cggaatgtcc
     2221 tgaacgaaga ggcgggagcc gtgccccggc tcgttgcccg aaaagagaat ccggcgtccg
     2281 tcaggaaacc aggtcgccgc ctgggcccgg atatcggtct tcgggagcgt ctttggctct
     2341 ccgggtccgg tcggcaggag cgcgagctga gagctctccc cctcccgccg gatcgtcagg
     2401 gcccaccgtc catccggcga gaagccccac gcgccgccgt ccccgatgcg gacggcggga
     2461 gagccgtcga gcttgcgcag gtagacgcct tgcggctcga tgttctgttc ttcgaagaga
     2521 acggtcttgc cgtcgggtga gatgcccttg ggaaacgacc agttgagcca ggtgagattg
     2581 cgctcgactt tgtcggccgc ggaaacgccc acgatctcgc ggcgagaaga gttcacggca
     2641 cagagaaccc gcccgtcgcg cgcgacgtcc cggaggaatc ccgcgggaac gttccagatc
     2701 atccgggtct tgcccgaaag caaagtcgcc gagatgctgt cgcgtccgct cgaccagact
     2761 tcgttccctc gcggagacca cgcgagcccg gagatcgcga acgggccggt caaccgcact
     2821 ttgccgtccg tgtcgacgac cttcacgttg ccgacgttgt ctccgcgcaa cgggtggtcg
     2881 atgaaggcga cgagacgccc gtcgggcgag acgcggacgt cgctcaccca gccgggcgcg
     2941 tcatacagca cctttccgat cgggtagtcg agccggttgc ggttccccac gtggcgcgcc
     3001 acggccaggc tcttgccgtc gggcgaccag tcggcatcct ccacgttttc gaggatctcg
     3061 cgcggcgcgc cgccggccag aggaacccgc gccagcgtcc caaccgtttc gaaaccgaac
     3121 atgaagtgac ggttcagcga gatcgccagc tcgccg
//
LOCUS       DASWAV010000557         3211 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U2665
            k141_713105, whole genome shotgun sequence.
ACCESSION   DASWAV010000557 DASWAV010000000
VERSION     DASWAV010000557.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960316
            Sequence Read Archive: SRR10260007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 3211)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3211)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:47:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,929
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3211
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_713105"
                     /isolate="SMAG_U2665"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.36444 N 132.31889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..425
                     /locus_tag="VF958_11645"
     CDS             <1..425
                     /locus_tag="VF958_11645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="cytochrome C"
                     /protein_id="HEX9149805.1"
                     /translation="AMSANRISRDLPFAKKKAVELLRAQYPDRDTAARKIPASFDEFY
                     SSNYPQQYQTHRALIESSGQQVSEIYLRNVFPNMKVTWGTYPNNIGHEDFLGCFRCHD
                     GNHKSADGKLISDDCEACHTLLAQEESNPKVLADLGLK"
     gene            470..757
                     /locus_tag="VF958_11650"
     CDS             470..757
                     /locus_tag="VF958_11650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9149806.1"
                     /translation="MKERGSRIGFVAVALFWAGWMAFTATSSATLPMQKKAKELGFAV
                     QDCTFCHTEKLPKKGAVTMNDRGKWLVAEKEKRGAKEVDVAWLKEYPGDKK"
     gene            781..1242
                     /locus_tag="VF958_11655"
     CDS             781..1242
                     /locus_tag="VF958_11655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018129590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske (2Fe-2S) protein"
                     /protein_id="HEX9149807.1"
                     /translation="MASNANTPGSGVLTRRSWASWLLGTSLGATVFAFLYPVLNYLVP
                     PESSEPSLSEFELDLKVSDIPPNSGRIVPIGGKPVLLFRKPSGELAALTAVCTHLDCT
                     VQFRADRSDIWCACHNGTYDLKGRNVSGPPPRPLTPLEIFVRGEKIVIKRA"
     gene            1235..2329
                     /locus_tag="VF958_11660"
     CDS             1235..2329
                     /locus_tag="VF958_11660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018129591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b N-terminal domain-containing
                     protein"
                     /protein_id="HEX9149808.1"
                     /translation="MPEPMTAEGASRGGLREWARDRLGWDDLAHIAAKKRVPVHRYTL
                     FYYLGGMALFLFLVQVATGILLLLYYRPSSAEAFESVQYVMADVPFGWLIRSIHSWGA
                     NLFVGVVFAHLISVFFLKAYLAPREVTWVTGAVLLFLALGFGFSGYLLPWNTLAFFAT
                     KVGTDVPAQIPGIGHLLSRILRGGSDVSGATLSRFYGIHVAILPAITTLILGAHLLLV
                     QRHGMSVPRSVEGKTRRSIPFVPDFLMRDMVGWLAAVGLLAALAAFFPWELGIKADPF
                     ASAPAGIRPEWYFLWMFQGLKYLPSKVGPIPGETLGVLFFGALAVAVVAVPWLDRRGK
                     RGIAIAAVLVLVVAAVLTVLALLPPPAAKP"
     gene            2326..>3211
                     /locus_tag="VF958_11665"
     CDS             2326..>3211
                     /locus_tag="VF958_11665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9149809.1"
                     /translation="MTRILLVAVLGLGASSAVAQSPPKAPQPVTGCVECHLALDDARI
                     SPPAKLFADDVHAKAGFTCVFCHGGDGRQEDQEKAHDPAKGFIGKPKPQQIPELCGKC
                     HSDAALMKQYDPSQRVDQLTEYRTSGHGKALVRGDTTVAQCASCHGAHGIQPVKDSRS
                     PVSPTKVAQTCNHCHGDAALMSAHKLPSDPYEKWSRSVHYRARVEKGDLSAPTCNSCH
                     GNHGAAPPQVGSVANVCGTCHVTFADQFKLSPHWEAFKEMELPGCVTCHENHEIVKPS
                     EAFLAEGDQGKCSSCHEAG"
BASE COUNT          547 a         1053 c         1031 g          580 t
ORIGIN      
        1 aagcgatgtc tgcgaatcgg atcagtcggg acctgccgtt cgcgaagaag aaggcggtcg
       61 agctgctccg ggcccagtac ccggatcgcg atacggccgc gcggaagatt ccggcttcgt
      121 tcgatgagtt ctacagctcg aactatccgc agcagtacca gactcaccgg gctctgatcg
      181 agtcgtcggg ccagcaggtc agtgaaatct acctgcggaa cgtcttcccc aacatgaagg
      241 tcacgtgggg aacctatccg aacaacatcg gacacgagga ctttctgggc tgcttccgct
      301 gccatgacgg caatcacaag agcgccgacg ggaagctcat ctcggacgac tgcgaggctt
      361 gtcatacgct gctggcgcag gaggaatcga atcccaaggt tctggcagat ctgggactga
      421 agtaggggac caaactgtta gagtgaatga gaaaaccgga gggaaaaaaa tgaaggagag
      481 aggatctcgg atcggtttcg tggcggtcgc cctcttctgg gcggggtgga tggcgttcac
      541 ggcgacttcg tcggcaacac ttccgatgca gaagaaggcg aaagagctgg gttttgcagt
      601 gcaggactgc acgttctgcc acaccgagaa gttgcccaag aagggcgccg tgacgatgaa
      661 cgaccgcgga aagtggctcg tcgcggagaa ggagaagagg ggcgccaaag aggtcgacgt
      721 ggcctggctc aaggagtatc ccggcgacaa gaagtgagga gctcgtagcg ggaaccgact
      781 ttggcatcga acgcgaacac acccggctcc ggcgtcctga cgcgccggag ctgggcatcc
      841 tggcttctcg ggacgagtct cggagcgacc gtcttcgctt ttctctaccc ggttctcaat
      901 tacctcgttc cccccgagtc ctccgagccg tcgctctcgg agttcgagct cgatctcaag
      961 gtttcggaca tcccgccgaa ctccgggcgg atcgttccca tcggcggaaa accggtcctt
     1021 ctttttcgaa aaccttccgg agagctcgcg gcgctcaccg ctgtctgcac gcatctcgac
     1081 tgcacggtgc aattccgcgc ggaccgttcc gacatctggt gcgcctgcca caacggtacc
     1141 tacgacctca aagggcgaaa cgtgtccgga ccgccgcccc ggcctctcac tccgctcgag
     1201 atctttgtcc gaggcgagaa gatcgtcatc aagcgtgcct gagccgatga ccgcggaggg
     1261 cgcgagccgt ggtggcctgc gcgagtgggc ccgggatcgg ctcgggtggg atgatcttgc
     1321 gcacattgcc gcaaagaagc gcgtccccgt ccaccgctac acgctcttct attacctcgg
     1381 cgggatggcg ctcttcctct tcctcgtgca ggtcgcgacc gggatcctcc ttctcctcta
     1441 ctaccgcccg agctccgcgg aggcgttcga gagcgtccaa tatgtgatgg cggacgtgcc
     1501 cttcggctgg ctcatccgct cgatccactc gtggggcgcg aacctcttcg tgggcgtcgt
     1561 gttcgcccac ctgatctccg tgttcttcct gaaggcatat cttgctcccc gcgaggtgac
     1621 ctgggtcacg ggggcggtcc tcctcttcct cgcgctgggg ttcggcttct ccggctacct
     1681 gctcccgtgg aacacgctcg ctttcttcgc gacaaaggtc gggaccgacg ttcccgcaca
     1741 gattcccggc atcggccatc tgctctcgcg gatcctgcgc ggcgggtcgg acgtctccgg
     1801 cgcgacgctg tcgcgcttct acgggatcca cgtggcgatc ctgccggcga tcaccacgct
     1861 gattctcgga gcgcacctcc tcctcgtgca gcggcacggg atgagcgttc cacgttcggt
     1921 cgagggaaag acgcggcgct cgatcccgtt cgttcccgac ttcctcatgc gcgacatggt
     1981 cggatggctg gcggccgtcg gtctcctcgc ggcgctcgcc gcgttctttc cctgggagct
     2041 cggcatcaaa gcggatccct tcgcgtcggc gccagccggc atccgacccg agtggtattt
     2101 cctctggatg ttccaggggc tgaaatacct gccgtcgaag gtcgggccga ttcccggcga
     2161 aacgctgggg gtgctcttct tcggcgctct cgccgtcgcg gtcgtcgcag tgccgtggct
     2221 cgaccgccgg gggaagcgcg ggatcgccat cgcggcggtc ctcgtcctcg tggtcgcggc
     2281 agtgctgacc gtgctcgcgc tgcttccgcc gccggcggcg aagccgtgac ccgaattctt
     2341 ctcgtggccg tgctcggact cggggcgtcg tcggccgtcg cgcagtcgcc tccgaaggct
     2401 ccgcagcccg tgaccggctg cgtcgagtgc catctcgcgc tcgacgacgc gcgcatcagc
     2461 ccgccggcaa agctcttcgc cgacgacgtc cacgcgaagg ccgggttcac ctgcgtcttc
     2521 tgccacggcg gcgacgggcg ccaggaagac caggagaagg cccacgaccc ggcgaagggc
     2581 ttcatcggaa agcccaagcc gcagcagatt ccggagctct gcgggaaatg ccactccgac
     2641 gcggcgctca tgaagcagta cgacccgtca cagcgggtcg accagctcac cgagtaccgc
     2701 acgagtggcc acggcaaggc cctcgtgcgc ggcgacacga cggtcgccca gtgcgcgagc
     2761 tgccacggcg cccacgggat ccagcccgtc aaggactcgc ggtctccggt ctcgccgacg
     2821 aaggtggccc agacttgtaa ccactgccac ggcgacgccg ctctcatgtc ggcgcacaag
     2881 ttgccgtccg acccttacga gaagtggtcg cggagcgtcc actaccgtgc gcgggtcgaa
     2941 aaaggggacc tctccgcgcc gacctgcaat tcctgccacg ggaatcacgg cgcagcgccg
     3001 ccgcaggtgg gctccgtcgc gaacgtgtgc gggacgtgcc acgtgacctt cgccgatcag
     3061 ttcaagctct cgccgcactg ggaggccttc aaggagatgg agctgccggg ctgcgtcacc
     3121 tgtcacgaga accacgagat cgtgaagccg agcgaggcgt ttcttgcaga gggggaccag
     3181 ggcaagtgct cgagttgcca cgaggccggc t
//
LOCUS       DASWAV010000558         2796 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U2665
            k141_192660, whole genome shotgun sequence.
ACCESSION   DASWAV010000558 DASWAV010000000
VERSION     DASWAV010000558.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960316
            Sequence Read Archive: SRR10260007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2796)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2796)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:47:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,929
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2796
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_192660"
                     /isolate="SMAG_U2665"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.36444 N 132.31889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..790
                     /locus_tag="VF958_11670"
     CDS             <1..790
                     /locus_tag="VF958_11670"
                     /inference="COORDINATES: protein motif:HMM:NF012497.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HEX9149810.1"
                     /translation="RSSQFLSALLLAAAAVGGGLSIRPRGSVVSAPYVETTINTLAAF
                     GYDARRSSDGSIDVERKDPPPRSYSIPGDWSSALPFLAAAGVVGGRIEVTGLSWPSVD
                     ADARAVEALEAMGVDIERSDVSIVARAGRGRPDPASIVARDFPDAVPALAALAANAAG
                     ESRFGGIAHLRGKESDRLGALATLLERAGARAIAGEDELVVVGPRRPTAVATRLPTFE
                     DHRIAMAAALLSLSSEGTLIENPDCVAKSYPGFFRDLETILVRG"
     gene            839..2131
                     /locus_tag="VF958_11675"
     CDS             839..2131
                     /locus_tag="VF958_11675"
                     /inference="COORDINATES: protein motif:HMM:NF012385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HEX9149811.1"
                     /translation="MNPSSGPPSDVLRALRDAVAAVASGQDREAPLGALGLVGLSILD
                     GDGRDVVQLGEPLTARRDVLRRSLDAGSARFELALAPPVADEVSAPIALLLETLLYRA
                     DGFAPPTDFADGWRRVGVVTADAVMREPYRRLLRFAPQVVTVLVLGESGSGKEAVARA
                     VHTLSPRAGGPFVSVNVPAIPPALLESELFGHARGAFTGAERDRRGLLEEATGGTIFF
                     DEIGDLSPALQAKLLRALQEREIRRVGENRSRAVDVRVVSATSRDLGKEIEAGRFRED
                     LYYRLHVGIITLPPLRDRGRDALLLGRHFLDRYAKEFGRGRLRFTPDAQTTLLSHRWP
                     GNIRELQNAVCQAAALAEPGGAVSAELLPSSVRRVFRPATAIGDYRTRVAAHRRRLVA
                     EALERSGGNRTRAAKELGLSRQALLYLMKELGIVTPRP"
     gene            2210..>2796
                     /locus_tag="VF958_11680"
     CDS             2210..>2796
                     /locus_tag="VF958_11680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9149812.1"
                     /translation="MNLGRRRLLPDARAALLIVACVVCYANGLTGDFTYDDKAIVRDN
                     PRLRAPAGLVSIFTTSYFGGPRGQGTGYRPILLVTYAIQRWLHGGAVFGFHVVNLALH
                     VIVTLLLARLLAQLGVPDTVAFGAALLFAVHPIHVEAVTSLVGRGETLAAAFVLGVLL
                     LALRYRRERRARSAVLALLLLCYALGVLSKESAAVA"
BASE COUNT          368 a          983 c          960 g          485 t
ORIGIN      
        1 tcgctcgagc cagtttctct cggcattgct cctcgcggcc gccgccgtcg gcggagggct
       61 ttcgatccgc ccgcggggaa gcgtcgtttc ggcgccgtac gtggagacca cgatcaacac
      121 cctggccgca ttcggttacg acgcgcgccg gagctccgac gggtcgatcg acgtcgagcg
      181 gaaggatccc cccccgcgct cctattcgat accgggcgac tggtcctccg ctttgccgtt
      241 tctcgcggcg gcgggagtcg tgggtggtcg gatcgaggtt acggggctct cgtggccatc
      301 ggtggatgcc gacgcgcgtg cggttgaagc gctcgaagcg atgggcgtcg atatcgaacg
      361 ctcggatgtt tccatcgtcg cgcgcgccgg gcgggggagg cccgaccccg cgtcgatcgt
      421 cgcccgggat ttccccgacg ccgtgccggc tctcgccgca ctggcagcga atgccgccgg
      481 ggagagccgt ttcggcggca tcgcccatct gcggggcaag gagagcgacc ggctgggcgc
      541 tctcgccacg ctcctggaga gggcgggcgc gagagcgatc gccggagagg acgagttggt
      601 cgtcgtcgga ccccggaggc caacggccgt cgcgacacgc cttcccacct tcgaggacca
      661 ccgaatcgcg atggccgccg cgctgctctc gctctcctcg gaagggactc tgatcgagaa
      721 cccggactgc gtcgcgaagt cgtatccggg attcttccgg gacctcgaga caatccttgt
      781 acgtgggtag agcgttctcg tccccggaac atcgccctcc aacctcgact ttcaggcttt
      841 gaacccctcc tccggcccgc cgtccgacgt cctgcgcgcc ctgcgcgacg cggtcgccgc
      901 cgtcgcctcg ggacaggatc gagaagcgcc gctcggagcg ctcgggctcg tgggcctttc
      961 gattctcgac ggggacgggc gcgacgtcgt gcagctcggc gaaccgttga ccgcgcgacg
     1021 cgacgtcctg cgtcggtcgc tggacgccgg aagcgcgcga ttcgagctcg cgctggcgcc
     1081 gcccgtcgcc gacgaggttt cggccccgat cgccctcctg ctcgagacgc tcctctatcg
     1141 cgccgacggc ttcgcaccgc cgaccgactt cgccgatgga tggcgtcggg tgggagttgt
     1201 gaccgccgac gccgtgatgc gtgagcctta ccgacgcctg ctgcggttcg ccccgcaggt
     1261 cgtgacggtc ctcgtgctcg gggagtccgg aagcggcaag gaagcggtcg cgcgcgccgt
     1321 ccacacgctc tctccgcgcg ccggcggtcc attcgtctcg gtgaacgtgc cggcgattcc
     1381 cccggcgctg ctcgagagcg agctgttcgg tcatgcgcgc ggggcgttca ccggggcgga
     1441 gcgggatcgc cgcggccttc tcgaagaggc gacgggagga accatcttct tcgatgagat
     1501 tggcgacctg agcccggcgc tccaggcaaa gctcctccgc gcgctccagg agcgggagat
     1561 ccggcgcgtg ggggagaacc gctctcgcgc ggtggacgtg cgcgtcgtgt cggcgacgtc
     1621 tcgcgacctc ggaaaagaga tcgaggcggg tcggtttcgc gaagacctct actaccgcct
     1681 gcacgttggg atcatcacgt tgccgccgct tcgggaccgc gggcgtgacg ccctccttct
     1741 cgggcggcac tttctcgacc gctacgcgaa agagttcggg cggggacgac ttcggttcac
     1801 gcccgacgct cagaccactc ttctctcgca ccgctggccc ggcaacatcc gcgagcttca
     1861 gaatgccgtc tgccaggccg ccgcgctggc ggagccgggc ggagccgtga gcgcggagct
     1921 ccttccgtcg tccgttcgtc gcgtctttcg gccggcgacg gcgatcgggg actaccgcac
     1981 gcgtgtcgcc gcccaccggc gtcggctcgt cgccgaagcg ctcgaacggt ctggcgggaa
     2041 ccgtacccgc gcggcgaagg agctgggcct ctcccggcag gcgcttctgt acctgatgaa
     2101 ggagctcggg atcgtaacgc cccgcccgta ggggacagac cccttttcac agttcacaca
     2161 gcgtctgtcc cagcgaaatg gggtctgtcc ccggtagagt tcgccgccga tgaacctcgg
     2221 tcggcgacga cttctgccgg acgctcgcgc ggctcttctc atcgtggcgt gcgtcgtctg
     2281 ctacgcgaac ggcctgacgg gcgacttcac ctacgacgac aaggcaatcg tccgggacaa
     2341 cccgcgtctc cgcgcgccgg cgggtctcgt ctcgatcttc acgacgtcat acttcggcgg
     2401 tccgcggggg caggggacgg ggtatcggcc gatccttctc gtcacgtacg cgatccagag
     2461 gtggctgcac ggcggcgccg tcttcggctt tcacgtcgtc aacctcgcct tgcacgtcat
     2521 cgtcacgctg ctcctcgcgc ggctgctggc gcaactcggc gtcccggata cggtggcctt
     2581 cggagctgcg ctcctcttcg cggtccaccc gatccacgtg gaagccgtca cgagcctcgt
     2641 cggacggggc gaaacactcg cggcggcctt cgtgctcggg gtgctcctgc tggcgctgcg
     2701 atatcggcgc gagcggaggg cgcgaagcgc cgtcctcgca ctcctcctcc tctgctacgc
     2761 gctcggggtc ctttcgaagg agagcgccgc ggtcgc
//
LOCUS       DASWAV010000559         1861 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U2665
            k141_312570, whole genome shotgun sequence.
ACCESSION   DASWAV010000559 DASWAV010000000
VERSION     DASWAV010000559.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960316
            Sequence Read Archive: SRR10260007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 1861)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1861)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:47:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,929
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1861
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_312570"
                     /isolate="SMAG_U2665"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.36444 N 132.31889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(41..985)
                     /locus_tag="VF958_11685"
     CDS             complement(41..985)
                     /locus_tag="VF958_11685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011465765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5996 family protein"
                     /protein_id="HEX9149813.1"
                     /translation="MSATSAVAWPELDLAKWADTRDTLHLWTQIVGKVRLVQTPWINH
                     SWHCSLYVTSRGLSTLPIPYGERIFQIDFDFIDQAVIVTTVDGERETIMLRPRTVASF
                     YQELMGILKDLELPVAIHKHASELPETIDLSQDVAHEAYDPESAQRFWRALASSHRVF
                     RQFRSRFLGKASPVHFFWGSFDLALALYSGRPAPPSEHAPPHMPKWVAQDASNREQAC
                     FGFWPGNVGGPVAEATFYAYAHPAPDGYSEARMRNPAARFSAEMGEWILPYDAVRNAK
                     DSDAEILAFLEDTYAAAADLGGWDRGALERKEELPPSK"
     gene            complement(982..1254)
                     /locus_tag="VF958_11690"
     CDS             complement(982..1254)
                     /locus_tag="VF958_11690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008480163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UBP-type zinc finger domain-containing protein"
                     /protein_id="HEX9149814.1"
                     /translation="METCSHVSEIRDVQPSADGCEDCLKIGDDWVHLRLCRECGHVGC
                     CDSSKNKHATKHFRKTRHPIVQSFEPGEDWLWCYVDEIFLKPAVAS"
     gene            complement(1297..1698)
                     /locus_tag="VF958_11695"
     CDS             complement(1297..1698)
                     /locus_tag="VF958_11695"
                     /inference="COORDINATES: protein motif:HMM:NF016839.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HEX9149815.1"
                     /translation="MSARKPAKPIREPRPLAAFFISLAVSALLLGGLLATDRRVMELR
                     KAKLELKEVGRKIADVRADNERLTAAIEAANREEFPAEKVAREELNLVHPEDLVLLYP
                     PGSLSGEKPTPAPPPATRPAATAAPPVSPNR"
     gene            complement(1695..>1861)
                     /gene="eno"
                     /locus_tag="VF958_11700"
     CDS             complement(1695..>1861)
                     /gene="eno"
                     /locus_tag="VF958_11700"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009213337.1"
                     /note="catalyzes the formation of phosphoenolpyruvate from
                     2-phospho-D-glycerate in glycolysis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HEX9149816.1"
                     /translation="DDSIADLAVAVNSGLIKTGSASRGERMAKYNRLLAIEEDLAEGG
                     VFAGRAAFPA"
BASE COUNT          292 a          637 c          636 g          296 t
ORIGIN      
        1 ggcagatgat cctcctcccc gccagcgggg agaggaaaaa tcatttcgac ggcggaagct
       61 cctctttacg ctcgagcgcg ccgcggtccc agccgcccag gtccgcggcc gcggcgtacg
      121 tgtcttcgag aaacgcgagg atctcggcgt cggagtcctt cgcgttccgc acggcgtcgt
      181 acggcaggat ccactccccc atctccgccg aaaaccgcgc ggccgggttt cgcatccgcg
      241 cctccgagta tccgtccgga gcggggtgcg cgtacgcgta gaacgtcgcc tcggccaccg
      301 gtccgccgac gttgcccggc cagaagccga agcacgcctg ctcgcggttc gaggcgtcct
      361 gcgcgaccca cttcggcatg tggggcgggg catgctccga cggcggcgcc ggtcgcccgg
      421 aatacagcgc cagggctagg tcgaagctcc cccagaagaa gtgcacggga ctcgcctttc
      481 cgagaaaacg ggaacggaac tgccggaaca ctcgatggga ggaagcgagc gcgcgccaga
      541 agcgctgcgc ggactccgga tcgtaggcct cgtgcgcgac gtcctgcgag agatcgatcg
      601 tctcgggcag ctcggaggcg tgcttgtgaa tcgccacggg aagctcgagg tccttcagga
      661 tccccatgag ctcctggtag aagctcgcga cggtgcgcgg ccgcagcatg atcgtctcgc
      721 gctcgccgtc caccgtcgtc acgatcacgg cctgatcgat gaagtcgaag tcgatctgga
      781 agatccgctc gccgtacgga atcggcaacg tgctcaggcc gcgcgacgtc acgtacaggc
      841 tgcagtgcca cgagtgattg atccacggcg tctggacgag ccggaccttc ccgacgatct
      901 gcgtccagag gtggagcgtg tcgcgcgtgt ccgcccactt cgcgaggtcg agctcgggcc
      961 acgcgacagc gctcgtcgcg ctcacgaagc gactgccggc ttcaggaaga tctcgtccac
     1021 gtagcaccag agccagtcct cgcccggctc gaacgactgg acgatcgggt gtcgggtctt
     1081 gcggaaatgc ttcgtcgcgt gcttgttctt cgacgagtcg cagcatccga cgtggccgca
     1141 ctcccggcag agccgcaggt ggacccagtc gtcgccgatc ttcagacagt cctcgcaacc
     1201 gtcggcgctc ggctggacgt cgcggatctc actcacgtgc gaacaggttt ccatggcgtt
     1261 ctcccgtgca cgtatgcggc caaccgcccg gccggttcag cgattcggcg agacgggggg
     1321 cgccgcggtc gcggcaggcc gagtcgcggg gggcggcgcc ggcgtcggct tctcgcccga
     1381 caggctcccc ggcggataga gcaggacgag gtcctcgggg tggacgaggt tcaactcctc
     1441 gcgcgcgacc ttctcggccg ggaactcctc gcggttcgcg gcctcgatcg cggcggtcaa
     1501 ccgctcgttg tccgcccgaa cgtcggcgat cttgcggccg acctccttca gctccagctt
     1561 cgccttccgc agctccatga cgcgccggtc ggtcgcgagg aggccgccga gaaggagcgc
     1621 cgacacagcg agcgaaatga agaacgccgc cagcggacgc ggctcgcgga tcggtttcgc
     1681 gggtttgcgc gcgctcacgc cggaaacgcc gcgcgccccg cgaagacgcc cccttcggcg
     1741 aggtcctctt cgatcgcgag gaggcggttg tacttcgcca tccgctcgcc gcgggacgcg
     1801 gagcccgtct tgatgaggcc tgagttgacg gcgacggcga ggtcggcgat cgagtcgtcg
     1861 g
//
LOCUS       DASWAV010000560         3098 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U2665
            k141_432432, whole genome shotgun sequence.
ACCESSION   DASWAV010000560 DASWAV010000000
VERSION     DASWAV010000560.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960316
            Sequence Read Archive: SRR10260007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 3098)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3098)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:47:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,929
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3098
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_432432"
                     /isolate="SMAG_U2665"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.36444 N 132.31889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1797
                     /locus_tag="VF958_11705"
     CDS             <1..1797
                     /locus_tag="VF958_11705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9149817.1"
                     /translation="EALSGSTSSATQQLIAPARGKPRWLAPAAGLAIALLIAGAFQVG
                     RRSGGRGAASTIPAMTFSQQTNQQGVESFPALSADGASLAFVSRETGNDDIYLQRVGG
                     HNPINLTKDSTEDDTQPAFSPDGSTIAFRSERGGGGIFIMGATGESVRRLSDLGYNPS
                     WSPDGKMIVVATEAVVNPLGRTGLSELWTIDVATGQKQKLFDGDAVQPSWSPHGDRIA
                     FWAIPKDTAQRDIWTIPSKGGAPVAVTQDAAVDWNPIWSADGKSLYFASDRGGSMNLW
                     RIAIDETSGRPTGVPQAFTAPSLWSGQLTISRDGRKVAYLALNELASIEKLPFEGSGK
                     TGSPVPLLRQSGSLFSPNISPDGEWIVFRSVGGQEDLFLVRSDGTGLRKLTDDPHRDR
                     GPSWSPDGKRIAFYSNRDGGKYEIWVINPDGGGLTELTKTSGRGWFPWWSPDGKKIAY
                     PTGSDSNVFDVAAGKTRAPEPLPAFPEKNHWFQVTSWSPDGRSLAGEGNVLASSGGGG
                     VVLYTFETRRYERLTQRGVKPLWLRDGRQLLFAPDQEHLALLDATSKQVRAVYTAPRG
                     SEIADFTISKDGRWFCVIRSNDEGDVWLAKLE"
     gene            complement(1808..3025)
                     /locus_tag="VF958_11710"
     CDS             complement(1808..3025)
                     /locus_tag="VF958_11710"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX9149818.1"
                     /translation="MKPWRDLRGMPRGVWVLFATTLVNRAGTMVLPFLVLYLTRDLGF
                     TAGQAGAVLTVYGAGALISAALSGRLSDVLGPMHVIRDSLFLTGVIHLLFPLARSHSA
                     VVAMTFALSLAAEAFRPASMAVVADLVKPAQRKPAFALTRLAINLGMSIGPALGGFLA
                     TVSFRSLFFVNGTCSLAAGLLLLLAMRRAPAHRGHAESEPGGPVELPLKRAWADSRLL
                     FFLAAVFPVAIVFFQHMSSMPLYLVRNLHLSELDYGLFFTINTLLIVALEVPINSATA
                     HWPHRRTLAIGAFLSGAGFGALALAWDFRSVAATVIIWTFGEMFLFPSLAAYVTDIAP
                     RARAGEYMGLTQMSMSLAFAVGPWAGTAVLEKFGGRTLWLACFALGLAATSMLLRLKE
                     NAPGHVEPVTRPV"
BASE COUNT          550 a         1025 c         1079 g          444 t
ORIGIN      
        1 gaggcactct ccggctcgac ctcgagcgcg acgcagcagc tgatcgcccc ggcccgcggg
       61 aagccgcgct ggctcgcgcc cgcggcgggt ctcgcgatcg cgctcctcat cgcgggcgcc
      121 ttccaggtgg gccggaggag cggcggccgt ggcgcggctt ccacgatccc cgcgatgacc
      181 ttctcgcagc agacgaacca gcagggcgtc gagagctttc ccgcgctgtc ggcggatggc
      241 gcgagcctcg cgttcgtcag tcgcgaaacg ggaaacgacg acatctatct ccagcgtgtc
      301 ggcgggcaca acccgatcaa cctcacgaag gactcgaccg aggacgacac acagcccgcg
      361 ttttctcccg acggctcgac gatcgcgttc cgctccgagc gcggaggcgg aggcatcttc
      421 atcatgggag cgacgggcga gtccgtgagg cgcctctcgg atctcggcta caacccgtcc
      481 tggtcgcccg acggcaagat gatcgtcgtc gcgacggaag ccgtggtcaa tcctctcggc
      541 cgcaccgggc tctccgagct ctggacgatc gatgtcgcga ccggtcagaa gcagaagctg
      601 ttcgacggcg acgccgtcca gccgagctgg tccccgcacg gggaccggat cgcgttctgg
      661 gccataccca aagacacggc gcagcgcgac atctggacga ttccctccaa gggcggggcg
      721 ccggtggcgg tgacgcagga cgccgctgtc gactggaacc cgatctggtc cgcggacggc
      781 aagtccctct acttcgccag cgaccgcggc ggcagcatga acctgtggcg aatcgcgatc
      841 gacgagacgt cggggcgccc gaccggcgta ccgcaggcgt tcacggcgcc gtcgctgtgg
      901 tcgggtcagc tcacgatctc gcgcgacggc cgaaaggtcg cctacctcgc gctgaacgag
      961 ctcgcgagca tcgagaaact cccgttcgaa ggatccggga agacgggatc ccccgtcccg
     1021 ctcctccggc aatccggatc gctcttttcg ccgaatattt cgcccgacgg cgagtggatc
     1081 gtctttcgct ctgtcggcgg ccaggaggac ctcttcctcg tccgatccga cgggaccgga
     1141 ttgcgcaagc tgacggacga tcctcaccgt gatcgcgggc cctcgtggtc gcctgacgga
     1201 aagcggatcg ccttctactc gaaccgcgac ggcggcaagt acgagatctg ggtgatcaat
     1261 ccggacggcg gtgggctgac cgaactgacg aagacgtccg gccgcggatg gtttccgtgg
     1321 tggtcgcccg atggaaagaa aatcgcgtat cccactggct cggattcgaa cgttttcgac
     1381 gtcgcagcgg gaaagactcg cgcgccggaa ccccttccgg ctttcccgga gaaaaaccac
     1441 tggttccagg tgacctcctg gtccccggac gggcgctcac tggccgggga agggaacgtg
     1501 ctcgcgagct cgggcggggg cggcgtcgtt ctctatacgt tcgaaacccg gcgatacgag
     1561 cgcctgaccc agcggggcgt caagcctctg tggctgcgcg acggtcggca gcttctgttc
     1621 gcgccggacc aggagcacct ggccctgctc gacgcgacgt cgaagcaggt gcgcgcggtg
     1681 tacacggcgc cgcgcgggtc cgagatcgcc gacttcacga tctcgaaaga cggccgctgg
     1741 ttctgcgtca tccggtcgaa cgacgaggga gacgtctggc tggctaagct ggagtagatt
     1801 ccgcctccta aaccggcctc gtcaccggct cgacgtgacc cggcgcattc tccttcaaac
     1861 gcagcagcat cgaggtcgcg gcgaggccga gcgcgaagca cgcgagccag agcgttcggc
     1921 ccccgaactt ctcgagcacc gcggtccctg cccaggggcc gacggcgaac gccaggctca
     1981 tcgacatctg agtcaggccc atgtattcgc cggcgcgcgc ccgcggcgcg atgtcggtga
     2041 cgtaggccgc cagcgagggg aacagaaaca tctcgccgaa ggtccagatg atgacggtcg
     2101 ccgcgacgct ccgaaagtcc catgccaggg cgagcgcccc gaatcccgcg cccgagagga
     2161 acgcgccgat cgcgagcgtc cggcggtgcg gccagtgcgc ggtcgccgag ttgatcggca
     2221 cctccagcgc gacgatcagg agcgtgttga tcgtgaagaa gaggccgtag tcgagctccg
     2281 agaggtgcag gttgcgcacg aggtacagcg gcatcgacga catgtgctgg aaaaagacga
     2341 tcgcgaccgg gaaaaccgcg gccaggaaga agagcagccg ggagtcggcc cacgcgcgct
     2401 tcaacggaag ctccaccggc ccgcccggct ccgactccgc gtggccgcgg tgcgcgggcg
     2461 cccgccgcat cgcgagcagg agcaggaggc ccgcagcgag ggagcacgtc ccgttcacga
     2521 agaagaggga ccggaaagag acggtggcga gaaagccgcc gagcgccggc ccgatgctca
     2581 tgccgaggtt gatcgcgagg cgcgtcagcg caaacgcggg cttccgctgc gcgggcttca
     2641 ccaggtcggc gacgaccgcc atgctcgccg ggcggaaggc ctcggcggcg agcgacagcg
     2701 cgaacgtcat cgcgacgacg gcggagtgcg agcgcgccaa cgggaagagg aggtggatca
     2761 cgcccgtcag aaagagcgag tcgcggatga cgtgcattgg tccgaggacg tcggagagtc
     2821 gtcccgaaag cgccgccgag atgagtgcgc ccgcgccgta gacggtgagg acggcgcccg
     2881 cctgaccggc cgtgaatccg aggtcgcgtg tcaggtagag gacgaggaag gggagcacca
     2941 tggtgccggc gcggttgacg agcgtggtcg cgaagaggac ccacactccc cgcggcatcc
     3001 cgcgcagatc ccgccaaggc ttcattgcct tcgattgtgt cccaggtatt tgttcgagag
     3061 cggtcttcgg cggttcggga gaaagaggcg ccgccctt
//
LOCUS       DASWAV010000561         1605 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U2665
            k141_587897, whole genome shotgun sequence.
ACCESSION   DASWAV010000561 DASWAV010000000
VERSION     DASWAV010000561.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960316
            Sequence Read Archive: SRR10260007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 1605)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1605)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:47:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,929
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1605
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_587897"
                     /isolate="SMAG_U2665"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.36444 N 132.31889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..278
                     /locus_tag="VF958_11715"
     CDS             <1..278
                     /locus_tag="VF958_11715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014857212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051304 - chromosome separation [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HEX9149819.1"
                     /translation="SVLTTLLEKKLVTTAGRKAVVGTPFLYRTTLEFLVRFGLNELED
                     LPKPEELDEDLAATVEAEPGTTPSTALESVPTVEAVEEEPPEDTAGE"
     gene            275..>1605
                     /locus_tag="VF958_11720"
     CDS             275..>1605
                     /locus_tag="VF958_11720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9149820.1"
                     /translation="MMVGRVASIAAVGVVLVVAVSRAVGQSPVGEIHFANSGSPAAQP
                     AFLRGLAQLHNFEYDDAAAAFVEAEKADPGFAMAYWGEAMTFNHPIWMEQDREAARKA
                     LERLAPFPEARRSKAPTEREKAYLDAVEVLFGDGTKNERDMRYAEAMRSLSAKYPDDP
                     DAAAFSALAILGTAHQGREVPTYMRAAAILEELTCRYPNHPGAAHYLIHSYDDPAHAV
                     LGLRAARSYSRIAPDAAHAQHMTSHIFIAMGMWDDVVTANETAVAVVNRAREKKGLPP
                     VSCGHYPYWLEYGYLEQGRVADARRLLEGCRAQAGNADHSGHGGRPDPDKSPVGSLIQ
                     MWARYLVDTNDWTGDVARWDMPVGDAPARRVTYEWARGFGSARRGDSAEARAALSRLS
                     AARHDLDANLAKEENPGAWTKRAVILESGLQAMIDAAQGRGEQALATLRSTS"
BASE COUNT          280 a          562 c          541 g          222 t
ORIGIN      
        1 cctccgtcct gacgaccctg ctcgaaaaga agctcgtcac gacggccgga aggaaggcgg
       61 tcgtcggcac gccgttcctg taccggacga cgctcgagtt cctcgttcga ttcggcctga
      121 acgagctcga ggaccttccc aagccggagg agctcgacga ggacctcgcg gcgaccgtgg
      181 aggccgagcc cggcacgacc ccgtccacgg cgctcgagag cgtcccgacc gtcgaggccg
      241 tcgaggagga accgcccgag gacaccgcgg gcgaatgatg gtcggtcgag ttgcgtcgat
      301 tgccgcggtg ggggtcgtcc tcgttgtggc ggtgagccgt gccgtcggcc agagccccgt
      361 cggcgaaatc catttcgcga actcgggatc gccggccgcg cagccggcgt ttctccgcgg
      421 cctcgcgcag ctccacaact tcgagtacga cgacgcggcg gcggcgttcg tcgaggcgga
      481 gaaagccgac cccggcttcg cgatggcgta ctggggcgaa gcgatgacgt tcaaccatcc
      541 gatctggatg gagcaggacc gcgaagccgc gcgcaaagcg ctcgagaggc tcgcgccctt
      601 tccggaggcg cgccgctcga aagcaccgac ggaacgcgag aaggcgtacc tcgacgcggt
      661 cgaggtcctc ttcggtgatg ggaccaaaaa cgaacgagac atgcggtacg cggaggccat
      721 gcgatcgctc tccgcaaaat atccggacga cccggatgcc gctgccttct cggctctcgc
      781 gattctcggc accgcgcacc aggggcgcga agtccccacg tacatgcgag ccgcggcgat
      841 cctcgaggag ctcacgtgcc gttatccgaa ccacccgggc gccgcgcact atctgatcca
      901 ctcgtacgac gacccggcgc atgcagtgct cggcctgcgc gccgcccgca gctactcgag
      961 gatcgctccc gacgccgccc acgctcaaca catgacgtcg cacatcttca tcgcgatggg
     1021 gatgtgggac gacgtcgtga cggcgaacga gacggccgtc gccgtcgtca accgcgctcg
     1081 agagaagaaa ggccttcccc ccgtttcctg cgggcactac ccctattggc tcgagtacgg
     1141 atacctcgaa cagggccggg ttgccgacgc ccgacgcctc ctcgaagggt gccgcgccca
     1201 ggccggcaac gccgaccact ccgggcacgg cggcagaccg gatcccgaca aatcccccgt
     1261 gggatcgctc atccagatgt gggcgcgata cctcgttgac acgaacgatt ggaccggcga
     1321 cgtcgcccgc tgggacatgc ccgtcggcga cgctccggcg cggcgcgtca cgtacgagtg
     1381 ggcgagaggc ttcgggtcgg cgcgccgcgg agattccgcc gaggcccgcg cagcgctgtc
     1441 gcgtctgtcg gccgcgcgcc acgacctcga cgcgaatctc gcgaaagagg aaaaccccgg
     1501 agcgtggacg aagcgggccg tgatcctcga gagcgggctc caggcgatga tcgacgccgc
     1561 ccagggccgc ggcgaacagg cgctggcgac gctccgcagt acgtc
//
LOCUS       DASWAV010000562         5219 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U2665
            k141_351044, whole genome shotgun sequence.
ACCESSION   DASWAV010000562 DASWAV010000000
VERSION     DASWAV010000562.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960316
            Sequence Read Archive: SRR10260007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 5219)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5219)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:47:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,929
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5219
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_351044"
                     /isolate="SMAG_U2665"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.36444 N 132.31889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(146..685)
                     /locus_tag="VF958_11725"
     CDS             complement(146..685)
                     /locus_tag="VF958_11725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006577672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macro domain-containing protein"
                     /protein_id="HEX9149821.1"
                     /translation="MPARIEAAHLDITAARVDAIVNAANEALQLGAGVAGAIRRTGGP
                     TIQEECDRISHCDVGQAVVTGAGDLPARWVIHAVGPVWRGGNEGEEIQLASAVFSALK
                     RAEEMGAKSVALPMISTGVFGFPIEIATRITVAAARAFAQKARSVERIVFCLPDEEKY
                     GRVRSQLEETETPGGDRST"
     gene            complement(693..1772)
                     /locus_tag="VF958_11730"
     CDS             complement(693..1772)
                     /locus_tag="VF958_11730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009542232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0140663 - ATP-dependent FeS chaperone
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mrp/NBP35 family ATP-binding protein"
                     /protein_id="HEX9149822.1"
                     /translation="MSAATELNEEKVLEVLKGVRFPGLSRDIVSFGFVKDLTVGGGNV
                     SFRLEVQTSSARAADEIRRDATERLRSLPGVNAVTIRLDVREPAPLAPRGATGVPPPD
                     ATLLPDVRFKIAVASGKGGVGKSTVAANLALALSRLGSRVGLMDSDIYGPSQQMMMGI
                     EEKPFINEENQIVPVEKFGIRVMSLGFLMDVDQPVIWRGPMVMKAVEQFLGDVAWGKL
                     DFLVVDLPPGTGDAHLTLTQKIHLSGAVIVTTPQDVALIDARKGLAMFQKVNVPVLGI
                     VENMSYYLCPKCGHREEIFKHGGGERTANSLNVPFLGEIPIDPKVAVGGDSGVPIVVS
                     DPKSAVTEAFLRVAEAIQKTLAGSA"
     gene            complement(1791..2516)
                     /locus_tag="VF958_11735"
     CDS             complement(1791..2516)
                     /locus_tag="VF958_11735"
                     /inference="COORDINATES: protein motif:HMM:TIGR00530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HEX9149823.1"
                     /translation="MRAVRSAIVWPLIALATVAFGVPAIFAALLPPRGDWFLRFARGW
                     ARTSLAIAGVSVRVLHADRLTADRSFVIVANHESFCDILVLLAHLPMQVRFLAKRSVF
                     RVPVLGWSIAAAGFIPVDRGDRSRGAETVETALRRLSGGRSVVIFPEETRTRTGDLLP
                     FKRGAAHLALKSGLPILPVALAGTYRVLKRGSLVITPGAVAMSVGDPIEVSGQTMHDR
                     ETITRAARDEVQALRAEARRAVA"
     gene            2628..3461
                     /locus_tag="VF958_11740"
     CDS             2628..3461
                     /locus_tag="VF958_11740"
                     /inference="COORDINATES: protein motif:HMM:TIGR00056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX9149824.1"
                     /translation="MPPTTTQQTATTIFYRNRRWLRFVDRFGKKGAGIVEYLGGLGTL
                     LAQTSRAAVTRPFYGSEVVSQMDELGVKSLSITGITAFSVGMVLALQTAYALAAFGGK
                     MFIGQVVAVSLVRELGPVLTSLMVGGRVGSGITAELGSMTVTEQVDALRSMAISPVRR
                     LVLPRVLSVVIMLPILTAIADVLGILGGLFIAVVDLQLTPSDYLNSIWNHLAIQDITS
                     GIAKTVFFGLEVAIIGCYNGLQVSGGASSVGQATTRTVVLASISILISDFFLTKLFLS
                     I"
     gene            3458..4294
                     /locus_tag="VF958_11745"
     CDS             3458..4294
                     /locus_tag="VF958_11745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020383186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX9149825.1"
                     /translation="MKAELKLSKETGPAPVAQGEPIVEFENVWKAYAHREVLAGVSLK
                     ILKGEVLCVLGPSGTGKSVTLRHINGLEQPDKGDIRVFGESVVGLTEEQLSPVRRRVA
                     MLFQGGALFDSMNVEQNVGFPLVEHTNKTPKEIAELVAEKLTMVGLPGTQKKMPSELS
                     GGMRKRVALARSIALEPEIIMYDEPTTGLDPLTSEKIAQLIADLNARLKITSIAVTHD
                     IVAARTVADRIAFLHQGRFQFLGTFEEAARGGHPVLQEYFRSMGFPCGTGLYAAPAGG
                     GT"
     gene            4295..>5219
                     /locus_tag="VF958_11750"
     CDS             4295..>5219
                     /locus_tag="VF958_11750"
                     /inference="COORDINATES: protein motif:HMM:NF014523.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MlaD family protein"
                     /protein_id="HEX9149826.1"
                     /translation="MAIESPVGRRFRVGLVVLIALVFLMFGILMVGKRAHLFTSKLPY
                     HTQFDSASGLVSGNFVRLNGVTVGNVLGVNLSPDPADRSVRVVYEVDRKVAPRLRTGT
                     RASIKTIGLLGDKYVELVGGLPEEAVIPPDGEIKAARGGGLDDLLAGGGDLVADLGGI
                     ARSLKAILEKMEKGGVGQNLNSTLLSLSEVLGKINRGESLAGKLLADRQYGEQTGASL
                     SAAVTSLRNVFGKIEKDMASGNGMIPALLSDPEGKKRVYDLLDKLGATAGSLAAVSEN
                     LNTGKGALPTLLHDEAFSREFTGNLRSLSKRL"
BASE COUNT          913 a         1713 c         1735 g          858 t
ORIGIN      
        1 ggccggcaac agggagagaa tcaggatcgc gcaaagcacg cccgggatca taaccaagca
       61 tttcccgcga caagacttga gtgaaggcag gtgccagggg gattgcccgc gagagatcgc
      121 gcttcccggc gcgttgcgcc gagcgttaag tcgagcgatc cccgcctggc gtttcggtct
      181 cctcgagttg gctgcgaact cggccgtact tctcctcgtc gggtaggcag aagacgatcc
      241 tttcgacgct gcgagccttc tgcgcgaatg cgcgggcagc cgcaaccgta atgcgcgtgg
      301 cgatctcgat gggaaacccg aagacgccgg tcgagatcat ggggagggcg acggatttcg
      361 cccccatctc ctccgcccgc ttcaacgcgg agaacaccgc agaggcgagc tggatctcct
      421 ccccttcgtt gcccccgcgc catacgggtc cgaccgcgtg gatcacccag cgggcgggca
      481 ggtcaccggc gccggtcacg accgcctgac cgacgtcgca gtggctgatc cgatcgcact
      541 cctcctggat cgtcggcccg ccggttctcc ggatggcgcc ggcgacgccg gcgcccagct
      601 gaagggcttc gttggcggcg ttgacgatcg cgtcgacacg cgccgccgtg atgtccaggt
      661 gagcggcttc gattcgggcg ggcaaggaag cgtcaggccg atccggccag agtcttctga
      721 atggcttccg ccactcgcag gaacgcctcg gtgacggcgg acttcggatc ggagacgacg
      781 atcggaacgc cggagtcgcc gccgaccgcg accttcggat cgatcgggat ctccccgagg
      841 aatggcacgt tgaggctgtt cgcggtgcgc tcgcctccgc cgtgcttgaa gatctcctca
      901 cgatggccgc acttcggaca caggtagtag gacatgtttt cgacgatccc gagcaccggc
      961 acatttacct tctgaaacat cgccaggccc ttgcgggcgt cgatcagggc gacgtcctgc
     1021 ggcgtcgtca cgatgacggc gccggagagg tgaatcttct gcgtgagagt caggtgcgcg
     1081 tcgcccgtgc ccggaggcag atcgacgacc aggaagtcga gcttgcccca tgcgacgtcg
     1141 ccgaggaact gttccacggc cttcatcacc atcggtccgc gccagatcac gggctgatcg
     1201 acgtccatca gaaagccgag agacatcacg cggatgccga atttctcgac cggaacgatt
     1261 tgattttcct cgttgatgaa gggcttctct tcgatcccca tcatcatctg ctgcgacgga
     1321 ccgtagatgt ccgagtccat gagtcccacg cgcgagccga gccgcgacag cgccagagcc
     1381 aggtttgccg ccacggtcga cttgcccacg ccgccctttc cggaagcgac ggcgatcttg
     1441 aagcggacgt cgggaagaag agtcgcgtcg ggcggcggga caccggtggc gccgcggggc
     1501 gccagcggcg ccggctcccg gacgtcgaga cggatcgtga cggcattcac tccgggaagc
     1561 gacctcagcc gctccgtcgc gtcccgccgg atctcgtcgg ccgcgcgggc cgaggaagtc
     1621 tggacctcca ggcggaacga cacgttgccg ccgcccacgg tcaggtcctt gacgaagccg
     1681 aacgagacga tgtcgcgcga aagacctggg aaccggacgc ccttcaggac ttcgagcacc
     1741 ttctcttcgt tcagctcggt ggcggcagac atgtcagacg atcgaaagta tcacgcgacc
     1801 gcgcggcgcg cctcggcgcg caacgcttga acctcgtcgc gggcggcgcg ggtaatcgtt
     1861 tcccggtcgt gcatcgtttg cccggagacc tcgatgggat ccccgaccga catcgccacg
     1921 gctccgggcg tgatgacgag cgatccccgc ttcaagaccc gataggtccc ggcgagcgcc
     1981 acgggcagga tcggcaggcc ggatttcagc gcgagatgag ccgcgccgcg cttgaagggc
     2041 aggaggtctc cggtgcgcgt gcgggtttcc tccggaaaaa tgacgacgga gcgtccgccc
     2101 gagagtcgtc tcagagcggt ttcgaccgtc tcggctcccc gcgaacgatc cccccgatcg
     2161 accgggatga aaccggcggc cgcgatcgac cacccgagga ccggcacgcg gaagacgctc
     2221 cgtttcgcga gaaaccgaac ctgcatggga aggtgggcga gcagcacgag gatgtcgcag
     2281 aagctctcgt ggttggcgac gatgacgaag ctccggtccg cggtgagccg gtcggcgtgc
     2341 agcacgcgga ccgagacccc cgcgatggcg agagacgtcc gcgcccagcc gcgcgcgaag
     2401 cgcaggaacc agtcccctcg cgggggcagc agggcggcga aaatggcggg cacgccgaac
     2461 gcgacggtgg cgagggcgat gagcggccag acgatcgcgg aacgaacggc ccgcatcgag
     2521 cggattgtag tcgggccgac gcaccaacga ccggcccggc gcgcgcaact tccgggacga
     2581 gagagtttta cagcggcggc ggtgtccgat tagaatcgcg ccactcgatg cccccgacca
     2641 cgacccagca gacggcgacg acgatcttct accggaaccg tcggtggctg cgcttcgtcg
     2701 accggttcgg caagaagggc gcggggatcg tcgagtatct gggcgggctg ggcaccctgc
     2761 tcgcgcagac gtcccgggcg gccgtcacac gcccgtttta cggctccgaa gtcgtctccc
     2821 agatggacga gctcggcgtc aagtcgctgt cgatcacggg gatcacggct ttctccgtcg
     2881 gcatggtgct cgcgctccag acggcctacg cgctcgcggc cttcggcgga aagatgttca
     2941 tcggccaggt ggtcgccgtc tcgctcgtcc gcgagctcgg cccggtcttg acctccctca
     3001 tggtcggcgg gcgggtcgga tcgggaatca ccgcagagct cggatcgatg accgtgacgg
     3061 agcaggtgga cgctctgcgc tccatggcaa tctccccggt tcggcgcctc gtgctgcccc
     3121 gggtcctctc ggtcgtcatc atgctgccga tcctgacggc gatcgccgac gtgctcggta
     3181 tcctcggcgg cctcttcatc gcggtcgtgg acctccagct cacgccttcg gactacctga
     3241 acagcatctg gaaccatctg gcgatccagg acatcacgag cgggatcgcg aagaccgtct
     3301 tcttcggcct cgaggtcgcg attatcggct gctacaacgg gcttcaggtc agcggcggcg
     3361 catcgtcggt cggccaggcg actacccgca cggtcgtcct cgcttcgatc tcgatcctca
     3421 tctccgactt cttcctcacg aaactgttcc tctccatatg aaagcagagc tcaagctctc
     3481 caaggagacc gggcccgccc cggtcgcgca gggcgaaccg atcgtcgagt tcgagaacgt
     3541 ctggaaggcc tacgcgcatc gcgaagtgct ggccggggtc agtctcaaga tcctcaaggg
     3601 cgaggtgctg tgcgtcctgg ggccgtccgg aaccggaaag agcgtcacgc tgcgccacat
     3661 caacgggctc gagcagccgg acaagggaga catccgggtc ttcggtgaat ccgtcgtcgg
     3721 gttgaccgag gaacagctct cgccggtgag gcgccgcgtg gcgatgctct tccagggggg
     3781 cgcgctcttc gactccatga acgtcgagca gaacgtgggg tttccgctgg tcgagcacac
     3841 gaacaagacg ccgaaagaga tcgccgagct cgtcgcggag aagctcacga tggtcggcct
     3901 gccggggacc cagaagaaga tgccgtcgga gctctccggc ggcatgcgga aacgcgtcgc
     3961 gctcgcgcgc tcgatcgctc tcgaaccgga aatcatcatg tacgacgagc cgacgaccgg
     4021 gctcgacccg ctcacgtcgg aaaaaatcgc gcagctgatc gcggacctga acgcgcggct
     4081 caagatcact tctatcgctg tcacgcacga catcgtcgcc gcccggacgg tcgcggaccg
     4141 cattgcgttc ctccaccagg ggcgattcca gttcctcggg accttcgaag aggcggcgcg
     4201 cggcggtcac cccgtgctcc aggagtattt ccgctcgatg ggctttccgt gtggcacggg
     4261 cctctacgcc gcgcccgccg gaggaggaac ctagatggcg atcgagtcac ccgtcggccg
     4321 gcgctttcgc gtcggcctcg tcgtcctgat cgcgctcgtc tttctcatgt tcgggatcct
     4381 catggtgggc aagcgggcgc acctcttcac ctcgaagctc ccgtaccaca cgcagttcga
     4441 ttcggcgagc ggattggtat ccgggaactt cgtccggctg aacggcgtca cggtcgggaa
     4501 cgtgctgggg gtgaacctct caccggatcc ggcggaccga agcgtgcgcg tcgtgtacga
     4561 agtcgaccgg aaggtcgcgc cccggctccg aacgggcacg cgggcgtcga tcaagacgat
     4621 cggtctcctc ggtgacaagt acgttgagct cgtcggcggc ctccccgagg aagcagtcat
     4681 ccctccggac ggcgagatca aggcggcgcg cggagggggt ctggatgatc tcctcgcggg
     4741 cggcggggat ctcgtggcgg acctcggcgg gatcgcgcgg tccctgaaag ccatcctcga
     4801 gaaaatggaa aagggagggg tcggtcagaa cctgaattcg accctgttga gcctctccga
     4861 agtgctcggg aagatcaacc gcggcgagag cctcgccggc aaactcctcg cggaccgtca
     4921 atacggcgag cagaccggtg cgtctctttc cgcggcggtc acgtctctgc gcaacgtttt
     4981 cggcaaaatc gagaaggaca tggcgagcgg aaacgggatg atcccggcgc tgctctccga
     5041 tcccgaaggc aagaagaggg tttatgacct cctcgacaag ctcggggcca ccgcggggtc
     5101 tctcgcggcg gtctccgaga acctgaacac tgggaagggg gctcttccga cgctcctcca
     5161 cgacgaagct ttctcccgcg agttcacggg caacctccgg agcctgtcga agcgactgg
//
LOCUS       DASWAV010000563         1675 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U2665
            k141_389096, whole genome shotgun sequence.
ACCESSION   DASWAV010000563 DASWAV010000000
VERSION     DASWAV010000563.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960316
            Sequence Read Archive: SRR10260007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 1675)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1675)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:47:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,929
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1675
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_389096"
                     /isolate="SMAG_U2665"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.36444 N 132.31889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            44..286
                     /locus_tag="VF958_11755"
     CDS             44..286
                     /locus_tag="VF958_11755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9149827.1"
                     /translation="MKASFVRVVSLVGALALPTVLVAQANPPAAKSTTVEKSTAKTEV
                     KTATTGKGVVKKSSKKRRHHSKKTTQSQAAKPAAKK"
     gene            complement(350..1648)
                     /locus_tag="VF958_11760"
     CDS             complement(350..1648)
                     /locus_tag="VF958_11760"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX9149828.1"
                     /translation="MSSEAAIVSRLPSTVSRPPGPSLGARLALYGEVVRTGFIELWAH
                     KLRSMLTLTLLMLGVFALVVMTSVLDGVMDKVGTGFAGMSWDGTIMVAPKSPETTEEQ
                     KRFAMSPGLRYEDLPRLTAPHPKALAFLPRAQKRSAVRTPAGSERMWIVGVSHDYAQW
                     MNRPIGIGRGLTQDDQRRRSTVAVVGATLGSKLFGGSDPVGRDVTVEGQRFRIVGILA
                     AGQIFSDENYYDANGILIPIETYMDRIDPDHKLTHVAVKLASKKDLAEVSATMIGRAK
                     QAHHNIEDIEITDMAAELARSYQNFLDQMRGWTIVLMSLAGTVLLVGGVGVLSVMLIS
                     FSDRRYEIGLRKAMGASDHEVFIQFILEALVLASVGALAGTLAGAALCRALSPKFPYG
                     LIVNPVGLIMAWGIALALALVFGMYPAIRASRLSPMEAMR"
BASE COUNT          312 a          536 c          557 g          270 t
ORIGIN      
        1 gtggagtccg aaattactcg cgcactccca gggaggcaga caaatgaaag catcattcgt
       61 tcgggtcgtt tcgctcgtcg gagccctcgc gcttccgacc gttctcgtcg ctcaggccaa
      121 tccgcccgct gcgaagtcga cgaccgtcga gaagtcgact gccaagacgg aagtgaagac
      181 ggcgaccacc ggcaagggcg tggtcaagaa gtcttcgaag aagcgccgtc atcattccaa
      241 gaagacgacg cagagtcagg cggcgaagcc ggcggcgaag aagtagtttc gtctcgagac
      301 ggaaggaaaa agccgggcac tgcccggctt tttgctcttc accgatcgac tagcgcatcg
      361 cctccatcgg tgacagacga gacgcccgaa tcgccggata cattccgaag acgagcgcga
      421 gagcgagagc gattccccac gccatgatca ggcccacggg gttgacgatc agcccgtacg
      481 ggaacttcgg ggagagcgcc cggcagaggg cggcgcccgc cagcgttccc gcgagcgcgc
      541 ccaccgacgc caggacgagc gcttcgagaa tgaactggat gaagacctcg tggtcggagg
      601 cgcccatcgc cttgcgcagc ccgatctcat agcgccggtc ggagaacgaa atcagcatga
      661 ccgacagcac cccgacgccc ccgacgagca ggaccgtccc cgcgagactc atcaagacga
      721 tcgtccagcc gcgcatctgg tcgaggaagt tctggtacga gcgcgcgagt tccgcggcca
      781 tgtcggtgat ctcgatgtcc tcgatgttgt ggtgggcctg cttcgcgcgg ccgatcatcg
      841 tcgccgacac ttcggcgagg tcctttttcg aggcgagctt aacggcgacg tgggtgagct
      901 tgtggtcggg gtcgatccgg tccatgtagg tctcgatcgg gatcaggatg ccgttcgcgt
      961 cgtagtagtt ctcgtcgctg aagatctgcc cggccgcgag gatcccgacg atacggaagc
     1021 gctggccttc gacggtgacg tctctgccga ccggatcgga acctccgaag agcttcgatc
     1081 cgagcgtcgc gccgacgacc gcgaccgtgc tgcggcgcct ctggtcgtcc tgcgtcagcc
     1141 cgcggccgat gccgatcggg cggttcatcc actgcgcgta gtcgtgcgag acgccgacga
     1201 tccacatccg ctcgctgccg gcgggcgtgc ggaccgccga gcgcttctgc gcgcgcggga
     1261 gaaacgcgag agcctttgga tgcggcgccg tcaacctcgg gaggtcctcg tagcgcagtc
     1321 cggggctcat ggcgaagcgc ttctgttcct ccgtcgtctc cggagacttc ggcgcgacca
     1381 tgatcgttcc gtcccagctc atccccgcga agccggtgcc gactttgtcc atgacgccgt
     1441 cgagcacgga ggtcatcacg acgagcgcga agacgcccag catgaggagg gtcagcgtca
     1501 gcatcgaccg gagcttgtgg gcccagagct cgatgaagcc ggtgcggacc acctcgccgt
     1561 aaagggcgag ccgggccccc agcgaaggcc ccggaggccg ggagacggtc gacggaagac
     1621 gggagacgat ggccgcttcg ctactcatag tgcagcgctt ccatcgggga gaggc
//
LOCUS       DASWAV010000564         4592 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U2665
            k141_512819, whole genome shotgun sequence.
ACCESSION   DASWAV010000564 DASWAV010000000
VERSION     DASWAV010000564.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960316
            Sequence Read Archive: SRR10260007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 4592)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4592)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:47:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,929
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4592
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_512819"
                     /isolate="SMAG_U2665"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.36444 N 132.31889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2363)
                     /locus_tag="VF958_11765"
     CDS             complement(<1..2363)
                     /locus_tag="VF958_11765"
                     /inference="COORDINATES: protein motif:HMM:TIGR03663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flippase activity-associated protein Agl23"
                     /protein_id="HEX9149829.1"
                     /translation="MSEAETLGLNEAAAGKGSPPVTTADSARSAGRVPAIVVGLFVVA
                     AAFTRFYHLGDRPLHHDESIHAHQSYSLMKDGNWRYDPAYHGPFLYYANALVYRIVGV
                     NNTTARLLPAIFGLLLIAAAIPLSRWIGRRAAIAYALLVLISPHLTYFSRFIREDLYS
                     LVFTLGTVLAFQMFLETDRAKWLTLAAVSFALAGITKENAYMTGVLFVAYGVWAIAER
                     VARSKGRKGAFSETLSATLRWIRARILPLATAALVFLAIWALAYSAFGKYPKDWLAIP
                     KAIKYWMGQHSIGRIPGPPWYYIPQFLYYETSLVFAALFAFRRPEKRDSQFLRFVVFW
                     AIGSLLLYAWAREKVPWLTVHPLLPVTILAAVGLAGLWRDRQEKPARLALAVFAVLAA
                     VNASGMALANFRYGAHDVAREPKHAEILAYVQTTRDLVRALEPLELAKQKVQPGQPVI
                     TVAGEASWPLTWYLRDVPTKWVTRVEDATTPIIVSDWESNGGLEKQLADAYTATRVPI
                     RAWWFAERYQPDEKVPARPNFTDLARWWLFHEIWSPIGSQDATFYVRKDLQSSGPLAP
                     LNLRVQNTSARDYSGGTAAVVPPAKILGEPGSGPGQFAEPRGLATDARGNLYVADTKN
                     NRIQVFDTNRQFVRSLGTAGSADGQLKEPCGVTVDSDGSVIVADTWNHRIARFGADGA
                     WLGAFTDVDRGLFGPRAVLMSRDSLYVADTGNKRIVRFDRKWNRVGEWGTSGSGPGQF
                     VEPVGLAADAAGNVYVADTGNHRIQVFDGEGHFLREFAVFGWKDFYT"
     gene            complement(2360..>4592)
                     /locus_tag="VF958_11770"
     CDS             complement(2360..>4592)
                     /locus_tag="VF958_11770"
                     /inference="COORDINATES: protein motif:HMM:NF021570.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF2298 domain-containing protein"
                     /protein_id="HEX9149830.1"
                     /translation="GAGVLQFLRAHADPASGLARRLGASVPLGLAIGAQALTNAWDVP
                     LLGGLLLLTGLVAYLSDASPGAGNRIARTATSIAASAAVAVLFALSLWVRGGGPPGFG
                     RNAEPGARGVDVLLHYGLFIFLAMVWLAATISEALSRDPKTRRFRAVPYFAGVGLLAV
                     GLLSADALCIVAILLLLFAIFRLLERPEDRMSSAMIAAGFFLVLFTQRAFISDRMNTF
                     FKLYFEAWPLFAISTAVLVFGSASRTGSYERWPAVFRVSFLVLLAAALFTSVTIVRGA
                     LFAPGNPSRPQEARRFTLDGMRYLEKWRPGEYAAVEWLRRAVPGTPVLLEAHGASYQD
                     FSRISMLTGIPTVLGWEHHVKQRGNPESEVEARRADVERLFSSSDVAETAATLRKYHV
                     GYVYVGPLERQTYPPEGLRKFASHADLFQVAYENRDVQIYRVVGGDAEDVIAPVREAL
                     PVEKKGESPPQEEAEEPPKISETPISQAPPFSGMKEPRDAAVDGRGRIWIADFGNSRL
                     RIFSPEGGLLGGWGGLGSGTFALRQPCGIAIVGDDVYIADTWNGRIQAFTAKGEWKAK
                     ATDLFGPRGIAVSTDGRVWVTDTGNKRLIVYDGSLKEVQRIGKLGSGPLEFSDPAGIA
                     VGPSDLVYVADTGNSRIQILNDKGQFERTIRVPAWNRNVEPHLEVDEDGTIYASDPEG
                     NSLITFKPSGEMDSRRTVDGSNRPLARPTGVALDRKNRILYVVNSGNSTVSKWDLPGK
                     PKS"
BASE COUNT          861 a         1460 c         1542 g          729 t
ORIGIN      
        1 tccgtgtaga agtccttcca gccgaagacg gcgaattcgc ggagaaagtg tccctccccg
       61 tcgaagacct ggatgcgatg gttgccggtg tccgctacgt aaacgttgcc ggcggcgtcg
      121 gccgcgagac cgaccggttc aacgaactgg ccgggaccgc tgcctgaagt tccccactcg
      181 cccacgcggt tccacttcct gtcgaagcgg acgatccgct tgttcccggt atccgcgacg
      241 tagagagagt cgcgcgacat gagcacggcc cgcgggccga agaggccgcg atccacgtcg
      301 gtgaaagcgc cgagccaggc accatcggcg ccgaatcggg cgatgcggtg attccacgta
      361 tcagccacga tcacgcttcc gtccgaatcc accgtgacac cgcacggctc cttcagctgc
      421 ccgtcggcag agccggcggt gccgaggctc cgcacgaact gtcggttggt gtcgaacacc
      481 tggatccggt tgttcttcgt atccgcgacg tacagatttc cgcgcgcgtc ggttgcgagg
      541 ccccgcggct cggcaaactg gccggggccc gagcccggct cgccaaggat ttttgccggt
      601 gggacgaccg ccgcggttcc tccggagtag tcgcgggctg atgtgttctg gacccgcagg
      661 ttgagcggag cgagcggccc ggagctctgg aggtccttgc gcacgtagaa ggtcgcgtcc
      721 tgcgagccga tcgggctcca aatctcgtgg aagagccacc agcgcgcgag gtccgtaaag
      781 ttcggacgcg ccggaacctt ctcatcgggc tggtaccttt cggcgaacca ccaggctcga
      841 atcggaacgc gagtcgccgt gtaggcgtcc gcgagctgct tctcgaggcc gccgttgctc
      901 tcccagtcgc tgacgatgat gggtgtcgtc gcgtcctcga cccgggtgac ccacttcgtc
      961 ggaacgtcgc gcaggtacca ggtgagcggc cacgacgctt cccctgcgac cgtgatgacc
     1021 ggctggccgg gttgcacctt ctgcttcgcg agctcgagcg gctcgagcgc gcggacgaga
     1081 tcgcgggtcg tctgaacgta cgcgagaatc tcggcgtgct tcggctcgcg cgcgacgtcg
     1141 tgagcgccgt agcggaagtt cgcgagcgcc atgccactcg cgttgacggc cgccagcacg
     1201 gcgaacaccg cgagcgcgag ccgggccggc ttttcctgcc ggtcgcgcca gagcccggcg
     1261 agaccgactg ccgcgaggat cgtgaccggg agaagcggat gaacagtgag ccatggcacc
     1321 ttctcgcgcg cccaggcgta gaggagcaga gagccgatcg cccagaacac tacgaagcgg
     1381 aggaactgcg aatcccgctt ctcggggcgg cggaaggcga agagcgccgc gaagacgagc
     1441 gacgtttcgt aatagagaaa ctgcgggatg taataccagg gcggaccggg aatccgcccg
     1501 atcgagtgct gacccatcca gtacttgatc gccttcggta tcgcgagcca gtctttgggg
     1561 tacttgccga aggccgaata cgcgagcgcc cagatggcga ggaagacgag cgctgcggtg
     1621 gcgagcggga gaatccgcgc ccggatccag cggagggtcg cggaaagcgt ctcggagaag
     1681 gctcccttgc gacccttcga ccgggccaca cgctccgcga tcgcccagac gccgtacgcg
     1741 acgaagagaa cacctgtcat gtaggcgttc tccttcgtga tgccggccag cgcgaacgaa
     1801 acggcggcga gcgtcagcca cttcgcgcgg tcggtctcga gaaacatctg aaaggcgagg
     1861 acggtgccga gcgtgaagac gagggaatag aggtcctccc ggatgaagcg cgagaagtaa
     1921 gtcaggtgcg gcgaaatcag caccagcaat gcgtaggcga tcgcggcgcg acggccgatc
     1981 caccgtgaga gcggaatcgc cgcggcgatc aacagcaggc cgaagatcgc cgggaggagt
     2041 cttgccgtgg tgttgttgac cccgacgatc cggtagacga gcgcgttggc atagtagaga
     2101 aacggcccgt ggtacgccgg gtcgtagcgc cagtttccgt ccttcatcag actgtaggac
     2161 tggtgggcgt ggatcgattc gtcgtggtgg agcgggcggt cgccgagatg ataaaaacgg
     2221 gtgaacgccg ccgcaacgac gaagagcccg acgacgatcg cgggaacgcg gccggccgat
     2281 cgagcggagt cggcggtcgt gaccggagga gaacccttcc cggccgccgc ttcgttcaat
     2341 ccgagcgtct cagcttcact caactcttcg gctttcccgg aaggtcccac ttcgagacgg
     2401 tgctgtttcc cgagttgacg acgtaaagaa tacggttttt ccggtccagc gccacgccgg
     2461 tcggtcgagc gagcggccgg ttcgagccat ccacagtgcg ccggctgtcc atctccccgg
     2521 agggtttgaa cgtgatgaga gagtttccct ccggatccga ggcgtagatc gttccgtcct
     2581 cgtccacctc gagatgcggc tccacgttgc ggttccaggc aggaacccgg atcgtccgct
     2641 cgaattggcc cttgtcgttc agaatctgga tgcgcgagtt tcctgtgtcc gccacataga
     2701 ccagatcgga aggaccgacg gcgatgccgg cggggtctga gaactcgagc gggccggaac
     2761 ccaacttccc gatcctctga acctccttga gcgacccgtc gtaaacaatc agtcgcttat
     2821 tcccggtatc ggtgacccag acacgtccgt ccgtcgagac cgcaatgcca cgcggtccga
     2881 agaggtccgt cgccttagcc ttccactcgc ccttggcggt gaacgcctgg atgcgtccat
     2941 tccaggtatc cgcgatataa acgtcgtcgc cgacgatcgc gatgccgcac ggttgacgca
     3001 gcgcgaacgt cccgctgccg agaccgcccc acccgcccag gaggccgcct tcggggctga
     3061 agattcggag gcgggagttc ccgaagtcgg cgatccagat ccgccctcgt ccgtcgacgg
     3121 cagcgtctct cggctccttc atgccggaga agggaggagc ctgcgagatg ggagtttcgc
     3181 tgatcttggg aggctcttcc gcttcctcct gcggcggcga ctcgcctttc ttttcgaccg
     3241 ggagcgcttc gcgcacgggt gcgatgacgt cttcggcgtc tccgccgacg acccgataga
     3301 tctggacatc ccggttctcg taggcgacct ggaagagatc ggcgtgagag gcgaacttgc
     3361 gaagtccttc cggcggatag gtttgccgct cgagcgggcc gacgtagacg tatcccacgt
     3421 ggtacttacg aagcgtcgcg gcagtttccg cgacatcgga gctcgagaaa agccgctcga
     3481 cgtccgcgcg acgcgcctcg acctcggact ccgggttccc gcgttgcttc acgtgatgtt
     3541 cccagccgag gacggtcggt atcccggtga gcatcgagat ccgggagaag tcctggtacg
     3601 aggcgccgtg ggcttcgagg agcaccggag tcccggggac ggcgcgccgg agccactcga
     3661 cggccgcgta ctcccccggt cgccacttct ccaggtaccg cattccgtcc agggtgaagc
     3721 ggcgcgcttc ctgcggccgc gaggggtttc cgggcgcgaa cagcgcgcca cgcacgatcg
     3781 tcaccgacgt aaagagggcc gccgcgagca gaacgagaaa ggagacgcga aagacggccg
     3841 gccaacgctc ataggagccg gtccgtgaag cagaaccgaa cacgaggacg gccgtcgaga
     3901 tcgcgaagag cggccaggcc tcgaagtaga gcttgaagaa cgtgttcatc cggtccgaga
     3961 tgaacgccct ctgggtgaac agaacgagga agaatccggc ggcgatcatg gctgagctca
     4021 tgcggtcttc cggccgctcg agcagtcgga aaatcgcgaa cagaagcagc aggatcgcga
     4081 ctatgcaaag cgcatcggca gaaaggagcc cgaccgcgag aagaccgact cccgcgaaat
     4141 atggcaccgc gcgaaaccgg cgcgtcttcg gatcccgaga cagggcttcc gagatcgtcg
     4201 cagccaacca gaccatcgca agaaagatga agagcccgta atgcaggagc acgtcaaccc
     4261 ctcgcgcgcc aggctccgca ttccggccga agccgggcgg gcctccgccg cgcacccaga
     4321 gggaaagcgc gaacaagacc gcgactgcgg cgcttgccgc gatcgaagtt gcagtgcgcg
     4381 cgatccggtt tcccgcgccc ggcgaggcgt cggacaggta ggcaacgagc ccggtcagga
     4441 gcagtaagcc ccccagaaga ggtacgtccc acgcgttggt gagcgcctgc gcgccgatgg
     4501 cgagcccgag agggaccgag gcaccgaggc gcctcgcgag ccctgaggcc ggatcggcat
     4561 gtgcgcgaag gaattggagc acgccggctc cg
//
LOCUS       DASWAV010000565         4577 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U2665
            k141_389113, whole genome shotgun sequence.
ACCESSION   DASWAV010000565 DASWAV010000000
VERSION     DASWAV010000565.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960316
            Sequence Read Archive: SRR10260007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 4577)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4577)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:47:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,929
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4577
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_389113"
                     /isolate="SMAG_U2665"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.36444 N 132.31889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1089
                     /locus_tag="VF958_11775"
     CDS             <1..1089
                     /locus_tag="VF958_11775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9149831.1"
                     /translation="FLPPEFRSVYTSFAGVDRPGLRGAELALAAIALLAIGLWLLLAA
                     IVAERMRTRSPRAVRAVEMGSIAVLAVIPLLRLAPPAGIRGVADLLPPLVRAVPPLLF
                     ATAAVALARRLAGRGAPAVFSGVPLSVLLVSLSFAARLFLAAGYVGPYNGFYLPLPLL
                     VTAAVLWKGAERLPALVSPPPSAFPALVSAAFAIFFVARVAALALLYRHPAWSRVATP
                     AGSLFVPEPVAGATRATLRDLASRVRKGGAITGFPETGFFNWTLGLRNPLAQDQFFPG
                     HLDTAAEEAAIALLPRDPPDAIVYADVLTIGHGRAAFGRDYLPRLDRHVRENFATAAS
                     YGPGARPGARIGDPDFFVEIRVPVASGR"
     gene            1086..1991
                     /locus_tag="VF958_11780"
     CDS             1086..1991
                     /locus_tag="VF958_11780"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015244237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HEX9149832.1"
                     /translation="MRLDLLVSRRFDLSRRSARDAVRSGRVDVEGSPRDEPGLDVRQD
                     ADVRFDRDRPERRRVRSRLAVLVEDPLFVIVDKPAGLLTVQTAEHESDTLFARALSYL
                     QHRYRRRAWVGVVHRLDKDTSGALVFARTREALRDLQEKFRTHSIDREYVAIVEGDVR
                     AAGVFDAALVADRGDRRRGVARGGEPGKRAVTHYKPIERLGRATLVSVRLETGRTHQI
                     RVHFSEAGHPVVGDSVYRRRGAPARPVEASRQMLHARQLGFAHPKTGEPVRAVSPIPG
                     DFAAVLEDLRRGQKKRPDASGRVLE"
     gene            complement(2042..2671)
                     /locus_tag="VF958_11785"
     CDS             complement(2042..2671)
                     /locus_tag="VF958_11785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007675796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HEX9149833.1"
                     /translation="MRVRTGTNRFLVVGALLVGIVGLGGCATKRYVGDEVSKSSAASE
                     KRISEVQSQVEMAQSRVREHDTHLAELDKSTREALERAEAAGKLAEGKFVYSLVLSDD
                     AVKFPVNRHELSKEAQAKLTEFAEKLKGENKNVYLEIQGHTDASGGPEYNYTLGEARA
                     EAVRRFLSKQGIALNRMSTISYGEDEPVAKNTSRKGRSQNRRVVVVVLA"
     gene            complement(2694..3662)
                     /locus_tag="VF958_11790"
     CDS             complement(2694..3662)
                     /locus_tag="VF958_11790"
                     /inference="COORDINATES: protein motif:HMM:TIGR03436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HEX9149834.1"
                     /translation="MKRARLGLAGLVLALAGLPATAQNPPANPQPAQDAPTYSEEVGT
                     EYVLLPVLVFDKKGRFIDGLTKKDFRVRVDTIPVEIDEFDRDVGAPVSMAFLVDTSGS
                     MEIAGKLDHAKKAIRSIVQARLPGDDFALFAFSEGKVEMVADFSSDSAKLFRAVDGLE
                     AGGQTALFDAVAATPRLLKGRNAKRAILLFTDGVDNASKLDPTQMAEILQQVSTPVYA
                     FGMKNVMYDLLSEEQRRELFVDNLKMLSASSGGQMYLVGDEDLGPLARNVNSEVRKQY
                     ILGFSPSGQGELKYRVVFVSVVKKGNWIVRTRRGYRGTAPTSTASR"
     gene            3705..4424
                     /locus_tag="VF958_11795"
     CDS             3705..4424
                     /locus_tag="VF958_11795"
                     /inference="COORDINATES: protein motif:HMM:NF012797.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrmH family RNA methyltransferase"
                     /protein_id="HEX9149835.1"
                     /translation="MEPGPALVLVAPRVPGNLGSAARAAKNFGVTDVRLVEPACEPDE
                     EAFRLASGADERLLGMRRFPTLIDAVADFDAVVATSSLRGRTERRWLTLAELPEFLSS
                     LGAGARVAYVFGRERSGLTEDERSRATATLTLPTDPTFPTMNLAHALAVILAVHRAFG
                     PLVAPLAREEQLAPSRELEGAVEHWDRALEAIGFYDTGHRERSLRDWRRIVAARPLSM
                     REVAILRGVANRVLVSLRRRD"
BASE COUNT          658 a         1607 c         1489 g          823 t
ORIGIN      
        1 ttccttccgc ccgagtttcg aagcgtgtac acgtcgttcg ccggcgtcga ccggccgggc
       61 ctgcgcggcg ccgagctcgc ccttgccgcg atcgcgcttc tcgcgatcgg cctctggctc
      121 ctgctcgcgg cgatcgtcgc cgaacggatg cgcacccgat cgccgcgcgc ggtccgcgcg
      181 gtcgagatgg gatcgatcgc ggtgctcgcg gtgatccccc tgttgcggct cgcgcccccc
      241 gcgggaatcc gcggagtcgc ggacctcctg ccgccgctcg tgcgcgcagt tccccctctc
      301 ctcttcgcaa ccgcagccgt cgcgctcgcg cggcgcctcg cgggaagagg agccccggcg
      361 gtcttctccg gagttcccct ttccgttctg ctcgtgtcgc tctccttcgc ggcgcgactc
      421 tttctcgcgg ccggatacgt gggcccctac aacggcttct acctgcctct ccctctcctc
      481 gtcaccgcag cggtcctctg gaagggcgcg gagcggcttc cggctctcgt gtcgccgcct
      541 ccctccgcct ttcccgctct cgtctccgca gcgttcgcga tctttttcgt ggcccgagtg
      601 gccgcattgg cgctccttta ccggcacccg gcatggtcgc gcgtcgcgac gccggcggga
      661 tcgctcttcg tgcccgagcc ggtcgccggc gccacgcggg ccacgctccg cgacctcgcc
      721 tcgcgcgtgc gcaaaggcgg cgcgatcacc ggcttccccg agaccgggtt cttcaattgg
      781 acgctcgggt tgcgaaaccc gctcgcgcag gaccagttct tccccgggca cctcgatacc
      841 gcggcagagg aggccgcgat cgcgcttctc ccgcgcgatc ctcccgatgc gatcgtttac
      901 gcggacgttc tgacgatcgg ccacgggcgg gcagcgttcg gtcgcgacta ccttcctcgg
      961 ctcgaccgcc acgtccgcga gaacttcgcc acggccgcgt cctacgggcc gggcgcgcgg
     1021 ccaggagccc gcatcggcga tccggatttc ttcgtcgaga tccgagtgcc ggtcgcgtcc
     1081 ggacgatgag gctcgacctc ctcgtctcgc ggcgattcga cctctcgcgt cgatcggctc
     1141 gcgacgcggt ccgctccggc cgcgtggacg tcgaaggatc tccacgggac gaaccgggcc
     1201 tcgacgttcg gcaagacgcg gacgtgcgct tcgatcgcga ccgtccggag cggcgccgcg
     1261 tccgcagccg tctcgccgtt ctcgtcgagg acccgctctt cgtgatcgtc gacaagccgg
     1321 cgggcctgct caccgtgcag acggccgaac acgaatcgga cacgctcttc gcgcgcgcac
     1381 tctcctacct ccagcaccgg tatcgccggc gcgcctgggt cggcgtcgtg caccgcctcg
     1441 acaaggacac ctcgggggcc ctcgttttcg cccggactcg cgaagcgctg cgcgacctgc
     1501 aggagaagtt ccgaacacac tcgatcgacc gcgagtacgt ggcgatcgtc gaaggggacg
     1561 tccgcgcggc cggtgtcttc gatgctgcgc tcgtggccga ccggggggac cggcgccgcg
     1621 gtgtcgctcg cggcggcgaa ccggggaaac gcgcggtgac ccactacaag ccaatcgagc
     1681 ggctcgggag ggcgactctc gtgtcggtcc ggctcgagac cgggcgcacg caccagattc
     1741 gcgtgcactt ctcggaggcg ggacacccgg tggtgggcga ctccgtgtac cgccgacgcg
     1801 gcgcgccggc gcggcccgtc gaggcttcgc ggcagatgct gcatgcgagg cagctcgggt
     1861 tcgcgcaccc gaagaccggc gagccggtcc gggcggtttc accgatcccc ggcgacttcg
     1921 cggccgtgct cgaagacctt cgtcgcggac aaaaaaaacg cccggacgca tccgggcgtg
     1981 tccttgagta gtcgcgggga caggtctttt tgacctacac ccggattctc agtagtcgct
     2041 gctaggcaag aacgacgacg accacccgac ggttctgaga ccggcctttg cgcgaagtgt
     2101 tcttcgcgac gggctcgtcc tcaccgtagg agatcgtgga catccggttg agcgcgatcc
     2161 cctgcttgga gaggaaacgc ctcaccgctt cggcccgagc ctcacccagg gtgtagttgt
     2221 attccgggcc cccgctggca tcggtgtggc cctggatctc gaggtagacg ttcttgttct
     2281 cgcccttcag cttctccgcg aactccgtca gcttcgcctg agcctccttc gaaagctcgt
     2341 ggcggttgac ggggaacttc accgcgtcgt cggagagaac cagcgagtac acgaacttgc
     2401 cctcggcgag cttgcccgcc gcctccgcgc gctccagagc ctcgcgcgtc gacttgtcga
     2461 gctcggcgag atgggtgtca tgctcgcgga cgcgagactg agccatctcg acctgactct
     2521 ggacctcgct gatcctcttc tcggacgcgg cgctgctttt ggagacctcg tcgccgacgt
     2581 agcgcttggt tgcgcagccg ccgaggccaa cgatccccac aagaagcgcc ccgaccacca
     2641 ggaaacgatt ggtacctgtt cgaaccctca tgaagcctcc cttcatcgat cggctaacgg
     2701 gacgccgtag aggtcggggc tgttccccga taaccacggc gcgtccggac gatccagttt
     2761 cccttcttga ccaccgacac gaataccacc cggtatttta gctccccctg cccggagggc
     2821 gaaaacccca ggatgtactg ttttcgcacc tcggagttga cgttgcgggc gagcggcccc
     2881 aggtcctcgt cgcccaccag gtacatctgg cccccgctcg acgccgagag catcttcagg
     2941 ttgtccacga agagctcccg acgctgctct tcgctcaaaa ggtcgtacat cacattcttc
     3001 atgccgaacg cgtacaccgg cgtggacact tgctgcagga tctctgccat ctgagtcggg
     3061 tcgagcttgc tcgcgttgtc caccccgtcg gtgaacagga ggatcgcccg cttggcgttc
     3121 ctccctttga gaagccgggg ggttgccgcg actgcgtcga agagcgccgt ctgacctccg
     3181 gcctcgagcc cgtcgaccgc gcggaagagt ttcgccgagt cggaagagaa gtcggcgacc
     3241 atctcgactt tcccctcgga gaaggcgaag agagcgaagt cgtctcccgg cagccgggcc
     3301 tggacgatcg agcggatcgc cttcttcgcg tggtcgagct ttccggcgat ctccatgctc
     3361 ccggacgtgt cgacgagaaa agccatcgac actggggcgc cgacatcacg atcgaactcg
     3421 tcgatctcga cagggatcgt gtccactcgc acgcggaaat cttttttcgt gagaccgtcg
     3481 atgaaccggc ccttcttgtc gaagacgagc acgggaagga gcacgtactc ggtgccgacc
     3541 tcttcggaat aggtcggggc gtcctgcgcc ggctgcgggt tcgccggggg gttctgggca
     3601 gtcgcgggga gccccgcgag cgcgagcacg agaccggcga gtccgaggcg agcgcgtttc
     3661 atcttcaacc cataagtatg ggggaggggc ccataattcc tgcgatggaa ccgggtccgg
     3721 ctctcgttct cgtggccccg cgagtacccg gaaatctcgg ttccgcggcc cgcgctgcga
     3781 agaacttcgg cgtcacggac gtgcggctgg tcgagcccgc gtgcgagccg gacgaagagg
     3841 cgttccgtct cgcgtcgggc gccgacgaac gactcctcgg gatgcgacgg tttccgacgc
     3901 tcatcgacgc ggtcgcggat ttcgacgccg tcgtcgcgac gtcttctctc cggggccgga
     3961 ccgaacggcg atggctgact ctcgccgagc tgccggagtt cctctcgtcc ctcggagcgg
     4021 gggcgcgggt cgcgtacgtc ttcggccgcg aacggagcgg cctgacggag gacgaacgat
     4081 cccgcgcgac cgcgacgctg acgctgccga ccgacccgac ctttccgacg atgaacctcg
     4141 cgcacgcgct tgcggtcatt ctcgcggtcc atcgcgcgtt cggaccgctc gtcgcgccgc
     4201 tggcccggga ggagcagctc gcaccgtcgc gggagctcga aggcgccgtc gagcactggg
     4261 atcgcgcgct cgaggcgatc ggcttctacg acacgggcca ccgagagcgg tcgctgcggg
     4321 actggcggcg catcgtcgcc gctcgcccgc tgtcgatgcg cgaagtcgcg atcctgcgcg
     4381 gcgtcgcgaa ccgggtgctg gtgtcgcttc ggaggaggga ctagacgtac ggggtcaggt
     4441 ctccacttgc cacattgggg gtcaggtctc cacttgccac ttcgcttcgc gaagatggca
     4501 aggtcaccga gttcggagtg gggtcaggtc tccacttgcc actttgctac gcaaagatgg
     4561 caaggtcact taggcgt
//
LOCUS       DASWAV010000566         2614 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U2665
            k141_553054, whole genome shotgun sequence.
ACCESSION   DASWAV010000566 DASWAV010000000
VERSION     DASWAV010000566.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960316
            Sequence Read Archive: SRR10260007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2614)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2614)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:47:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,929
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2614
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_553054"
                     /isolate="SMAG_U2665"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.36444 N 132.31889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(16..639)
                     /locus_tag="VF958_11800"
     CDS             complement(16..639)
                     /locus_tag="VF958_11800"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005008565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0016018 - cyclosporin A binding [Evidence
                     IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HEX9149836.1"
                     /translation="MKIKVATAFLAICALAVSGQAAQKGKKKVAPRDYANTLAVLQTD
                     MGDITIKFSYDRAPKHVENFIDLAAKGFYDGTMFHRVIPGFMIQGGDPLTKKAEDPRH
                     PYGTGGNVDATGKENRLKAEFNDTSHRRGVVSMARASDPNSASSQFFVVVKDSPFLDH
                     QYTAFGEVVSGIEVADKIVSAPRGPNDRPNQPIRIKKVVLSQAKSLS"
     gene            725..1264
                     /locus_tag="VF958_11805"
     CDS             725..1264
                     /locus_tag="VF958_11805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9149837.1"
                     /translation="MYAALVRTVLTDADHDKIVEQVRKLRERYPHSSKQELAERLIRQ
                     TALRSALVGAVTSAPPGLFSVIPFATDVSYQAMALNRLMLAIAQVYRRPTSPLERAGA
                     VAASVVLEGGTEFLRRGIVRGVARSLRRQRAVRLIPLFGALLGGVATYVTVTAVGREA
                     NRLLRPRGIASRVRTVLNR"
     gene            1261..2367
                     /gene="thiO"
                     /locus_tag="VF958_11810"
     CDS             1261..2367
                     /gene="thiO"
                     /locus_tag="VF958_11810"
                     /EC_number="1.4.3.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR02352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016647 - oxidoreductase activity, acting
                     on the CH-NH group of donors, oxygen as acceptor [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine oxidase ThiO"
                     /protein_id="HEX9149838.1"
                     /translation="MIAGADVTVVGGGVIGLALARELSSRGAEVIVFERGLTGEEASS
                     AAAGLLTAQWEASAPSPFFDFAVQSRELYPRWADALEEETGLAVGWRPTGVLHCGSAE
                     HLEKFLWQVDAGQPVERVDAAEIRRRSAGRVAPGVSEALFFPRDSVVNNRLLVRALRR
                     SLEIRGVRVLEGMAVTRFLVEGGTCRGVETSGGTFAATRVVDAAGAWADLDPNLPFAI
                     PVEPIRGQIVELADDGPFSTVLASDDVYLVPRSDGRVLVGATAERAGFRKEVTAAGVG
                     KLLAAAFALVPSLGAARVSGMWAGFRPGTPDGLPLLGESPVAGLYLATGHFRYGILLA
                     PITALRIADLLTGVAVHDLAPFAPERFAVAARDR"
BASE COUNT          378 a          862 c          897 g          477 t
ORIGIN      
        1 ggttcgctgc cggtctcacg aaagcgattt cgcctgggag agaacaacct tcttgatgcg
       61 aatcggctgg ttcggtcggt cgttcgggcc ccggggagca gagacgatct tgtcggcgac
      121 ctcgatcccc gagacgactt cgccgaacgc ggtgtactgg tgatccagga atggggagtc
      181 cttcacgacg acgaagaact gggaggaagc cgagttcgga tcggacgcgc gcgccatcga
      241 gacgacgccg cgccggtggc tcgtgtcgtt gaactccgcc ttgaggcggt tttccttgcc
      301 agtggcgtcg acgttgccgc cggtcccgta cgggtgacgc ggatcttccg ccttcttcgt
      361 cagcggatcg cccccctgga tcatgaagcc gggaatgacc cggtggaaca tcgttccgtc
      421 gtagaaaccc ttcgcggcga ggtcgatgaa gttctcgacg tgcttgggcg ctcggtcgta
      481 agagaacttg atcgtgatgt cgcccatgtc ggtctggagg acggcgagcg tgttggcgta
      541 atcgcgtggg gccaccttct tctttccctt ctgggccgcc tggcccgaca cggcgagtgc
      601 gcagatcgcg agaaacgcag tcgcgacttt gatcttcaag gctgactcct ttcgagcgga
      661 ccgggcgaca ataacactgc agtcccgtgg ggaattccga attggcacgg taagatcggg
      721 cccgatgtac gccgcactcg tgcgcaccgt actgacggac gcggaccacg acaagatcgt
      781 ggaacaggtc cgcaagctgc gtgagcggta tccccattct tcgaagcagg agctcgcgga
      841 acgactgatc cgtcagacgg cgctgcggag tgcgctggtc ggcgcggtca cttccgcacc
      901 gcccggtctc ttttcggtga tccccttcgc cacggacgtc tcctaccagg ccatggcgct
      961 caaccgtctc atgctggcca tcgcgcaggt ctaccggcgc cccacgagcc ctctcgagcg
     1021 ggccggcgcg gtcgctgcca gtgtcgtcct cgaaggagga accgagtttc tgaggcgcgg
     1081 catcgtgagg ggggtggctc ggtcgctccg gcgacagcgg gccgtgcgcc tgatccctct
     1141 cttcggtgct ttgctcggcg gagtcgccac ctacgtcacg gtgacggcgg tcgggcggga
     1201 agcgaatcgg ctcctccggc cacgcggcat cgcgtccagg gtgcgcacgg tcctgaaccg
     1261 atgatcgcgg gcgccgacgt taccgtcgtc ggcggaggcg tgatcggcct cgcgctcgct
     1321 cgggagctct cttcgcgggg cgcggaggtc atcgttttcg agcgcggact caccggcgag
     1381 gaagcctctt ccgcggccgc gggcctcctg actgcccagt gggaggcgtc ggcgccttcg
     1441 ccgttcttcg acttcgcggt ccaaagccgc gagctctacc cgcgttgggc ggatgcgctc
     1501 gaagaggaga ccggcctcgc cgtcggctgg cgcccgaccg gcgtgctcca ctgcggcagc
     1561 gcagagcacc tcgagaagtt cctctggcag gtcgacgccg gccagccggt cgagcgggtc
     1621 gacgccgcgg agatccgacg gcgttccgca ggccgggtcg cgcccggagt atcggaggcg
     1681 ctcttctttc cgcgtgattc ggtggtcaac aaccggttgc tcgtccgcgc actccggcgc
     1741 tcgctcgaga tccgcggcgt gcgcgttctc gagggaatgg ctgtcacacg ttttctggtc
     1801 gagggaggaa cctgccgcgg cgtcgaaacg tcggggggga ccttcgccgc gacacgggtc
     1861 gtggatgccg cgggcgcgtg ggcggacctc gatccgaatc tccctttcgc gattcctgtc
     1921 gagccgatcc gcggtcagat tgtcgagctc gccgacgacg gcccgttctc gaccgtcctg
     1981 gcgtcggacg acgtctacct cgttccccgc tccgacggtc gcgtgctggt cggcgcgacg
     2041 gccgagcgag ccggctttcg caaggaggtc acggcggccg gcgtcggcaa gctcctggcc
     2101 gcggcgttcg ctctcgtccc gtccctcggg gcggcgcggg tgtccggcat gtgggccggc
     2161 ttccggccgg gtacgcccga tggcctgccg ctcctcggtg aatctccggt cgccgggctc
     2221 tacctcgcga cggggcactt ccgatacggg attctcctcg cgccgatcac ggcgcttcga
     2281 atcgcggacc ttctcaccgg tgtcgcggtg cacgacctcg cgcccttcgc accggagagg
     2341 ttcgccgtcg ccgcccgcga ccgatgaccg cgatatttcg gttagaatca cgagcaaccg
     2401 gtccggattc ttgccggaag ggggagcgga aaaaaacgga gggaaggcgg tctcgacgcc
     2461 gcctcaagtg ggaatcacta tgaaacaggc gatctactga ggggtgtcga aagccgcagg
     2521 cgttctctgg cgcgccgcgc gtgccttcga ccggcggttc ccgcaacggt cctttcaccc
     2581 gcgctgggcc ccggctccgc tccagaagca gaac
//
LOCUS       DASWAV010000567         2203 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U2665
            k141_193419, whole genome shotgun sequence.
ACCESSION   DASWAV010000567 DASWAV010000000
VERSION     DASWAV010000567.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960316
            Sequence Read Archive: SRR10260007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2203)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2203)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:47:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,929
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2203
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_193419"
                     /isolate="SMAG_U2665"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.36444 N 132.31889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..606
                     /locus_tag="VF958_11815"
     CDS             <1..606
                     /locus_tag="VF958_11815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9149839.1"
                     /translation="LKEPDSSGAAAALLALLLAFVVGSHRLAPEVSLLFFLLAAQVAI
                     GGETRRRGAVRPTTALIALYAAGAIFGILRTVDPRETFRHSRMIGFHELETGPGGHFR
                     WTRREFAIRVRAEAPEQISLANYSPEGKPVGITVRATGVERALYRRSLRPGEGVHLAL
                     WSGGQARVFRFELDSAFVPKRLTGSDDRRELGLLAVLPEGR"
     gene            complement(616..1788)
                     /gene="aroC"
                     /locus_tag="VF958_11820"
     CDS             complement(616..1788)
                     /gene="aroC"
                     /locus_tag="VF958_11820"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011341885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HEX9149840.1"
                     /translation="MRLLTAGESHGPRLTAILDGIPAGLPIDPAFIDRELARRQHGYG
                     RGGRMKIERDRAVFEGGVRGGVTIGSPIAIGIANRDFANWSKVMDPLDNDPAASAGRR
                     LTRPRPGHADLAGGMKYGANDLRDVLERASARESAARVAAGAVCKLLLRECGIDVKSG
                     VLSVGAAVDDRSPRSFDSLEKVRENSPMRALDAAIEKKMVAAVDAAKKEGDTLGGSVL
                     VGARGVPAGLGSYASWDRKLDSRIGGALLSIPAVKAVELGSSIEASRGPGSRAHDEIE
                     PARHGGLRRRTNRAGGLEAGVTNGEDVIAVVYMKPLSTLARGLDSVDLETGIASRAAY
                     ERSDVTAVAACGVIAEAMLAFVLADALLESTGGDRLEDVKFRLAAHRERVRRYPAP"
     gene            1877..>2203
                     /locus_tag="VF958_11825"
     CDS             1877..>2203
                     /locus_tag="VF958_11825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011553505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HEX9149841.1"
                     /translation="MKRYVLKTPVGPKTYRIDYDSELNEEQREVVLAGGGPILVIAGA
                     GSGKTRTLVYRVARLIESGTDPSRIMLLTFTNKAAREMLRRVEALLAVDTRRLMGGTF
                     HSAGNRL"
BASE COUNT          309 a          775 c          776 g          343 t
ORIGIN      
        1 ctcaaggagc ccgattcctc gggggccgcg gccgcgctac tcgcgcttct tctcgcgttc
       61 gtcgtcggtt cgcaccggct cgcgccggag gtgtcgttgc tgttttttct gctcgccgcg
      121 caggtcgcga tcggaggcga aacgcgccgg cgaggggcag tgcggccgac cacggcgctc
      181 atcgcgctct acgcggccgg ggcgatcttc gggatcttac gaacggttga tccgcgagag
      241 acgttccgcc actcccggat gatcggattt catgagctcg agaccggccc gggcgggcac
      301 tttcggtgga cgcgacgcga gttcgcgatc cgggtgcgtg cagaggcgcc ggagcagatc
      361 tcgctcgcga actactctcc ggaggggaag ccagtcggga tcacggttcg ggccacgggc
      421 gtcgagcgcg ccttgtaccg gcggtcgctc cgccccggcg aaggcgtcca cctggcgctc
      481 tggtcggggg gccaggcgcg cgtcttccgt ttcgagctcg acagcgcctt tgtcccgaag
      541 cgcctgacgg gatcggatga tcgcagagag ctcggtcttc tcgccgtcct gccggaaggc
      601 cgctgacggc ggttcctagg gagcgggata ccggcgcacg cgctcccggt gcgccgcgag
      661 gcggaacttg acgtcttcga ggcggtcgcc gccggtcgac tcgaggagcg catcggcgag
      721 cacgaaggcg agcatcgcct cggcgatgac gccgcacgct gccacggcag tcacgtcgct
      781 gcgctcgtag gctgcgcgcg aggcgatccc ggtctccagg tcgacggagt cgagcccgcg
      841 ggcgagcgtc gagagtggct tcatgtacac caccgcgatc acgtcctcgc cgttcgtcac
      901 gcccgcctcg aggcctccgg cgcggttggt gcggcgccgc agaccaccgt gcctggccgg
      961 ctcgatctcg tcgtgcgcgc gcgagccggg ccctcgggac gcctcgatcg aggagccgag
     1021 ttcgacggcc ttcaccgccg gaatcgagag cagcgcgccg ccgattctcg aatcgagctt
     1081 gcggtcccac gaggcgtacg agccgaggcc tgccggcacg ccgcgggcgc ccacgaggac
     1141 cgaccctccg agagtatcgc cctccttctt cgcggcgtcg acggctgcga ccatcttctt
     1201 ctcgatcgcg gcatcgagcg cgcgcatcgg cgagttttcg cggaccttct cgagcgagtc
     1261 gaacgagcgc ggcgaccggt cgtccacggc ggcgcccacc gagagaacgc cgctcttcac
     1321 gtcgattccg cactcgcgaa ggaggagctt gcagaccgcg cccgccgcga cgcgcgcggc
     1381 cgactcccgg gcggatgcgc gctccagcac gtcgcgcaga tcgttcgcgc cgtacttcat
     1441 ccccccggcc agatcggcgt ggcccggccg cggacgggtg agacgtcgcc cggccgaggc
     1501 cgcggggtcg ttgtcgagcg ggtccatcac cttcgaccag ttcgcgaaat cgcggttggc
     1561 gatgccgatc gcgatcgggg atccgatcgt caccccgccg cggacgccac cctcgaagac
     1621 cgcgcgatcc cgctcgattt tcatccggcc gccccgcccg tagccgtgct gtcggcgcgc
     1681 gagctcgcgg tcgatgaatg ccggatcgat ggggagtccg gcgggaatgc cgtcgaggat
     1741 cgcggttagg cgaggcccgt gcgattcgcc ggcggtcagg aggcggatca ttgcggagat
     1801 cgtgacacac cccgggcctc gccccaaccc tctgccagcg cggggagagg gtggcgaacc
     1861 cgctaaactc cgccgcgtga aacggtacgt tctcaagacg ccggtcgggc cgaagaccta
     1921 ccggatcgac tacgactccg agctgaacga ggagcagcgg gaggtcgttc tcgccggcgg
     1981 cggaccgatc ctcgtcatcg cgggcgccgg ttcggggaag acccgcacgc tcgtctaccg
     2041 cgtcgcgcgg ctgatcgagt cgggtaccga cccgtcgagg attatgctcc tgaccttcac
     2101 caacaaggcc gcccgggaga tgctgcggcg ggtcgaggct ctcctcgccg tggacacccg
     2161 gcgcctgatg gggggcacgt tccactcggc cggcaaccgt ctc
//
LOCUS       DASWAV010000568         7159 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U2665
            k141_671331, whole genome shotgun sequence.
ACCESSION   DASWAV010000568 DASWAV010000000
VERSION     DASWAV010000568.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960316
            Sequence Read Archive: SRR10260007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 7159)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7159)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:47:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,929
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7159
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_671331"
                     /isolate="SMAG_U2665"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.36444 N 132.31889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1077
                     /gene="atpA"
                     /locus_tag="VF958_11830"
     CDS             <1..1077
                     /gene="atpA"
                     /locus_tag="VF958_11830"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020707407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HEX9149842.1"
                     /translation="IDAMIPIGRGQRELVIGDRQTGKTAVALDTIINQKGKGLICVYV
                     VIGQKKSTVAQVVKTLEEYGAMEHTIVVSATASDPAPMQFIAPYAGCAMGEYFLYNKQ
                     HALTIYDDLSKHAAAYREISLLLRRPPGREAYPGDVFYLHSRLLERAAKLSDERGGGS
                     LTSLPFIETQAGDVSAYIPTNVISITDGQIYLESDLFYSGVRPAVNVGLSVSRVGGNA
                     QIKAMKQVAGTLRLDLAQFRELAAFAQFGSDLDKTTQAQLARGQRLVEILKQAQFAPM
                     DVELQIVSIYAGTKGFLDSLRVDAVLPFEAGLHDFVRREHSEVLSAIVNTGKLEKATE
                     EALAAAIAAFKDRFVRENADAVAA"
     gene            1093..1977
                     /gene="atpG"
                     /locus_tag="VF958_11835"
     CDS             1093..1977
                     /gene="atpG"
                     /locus_tag="VF958_11835"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011682250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit gamma"
                     /protein_id="HEX9149843.1"
                     /translation="MASLIDIRRRLKSVRNMRQITRAMKMVSAARLRRAQDRAIAARP
                     YAKALREVLASVSSRVKEAKHPLLGTREERQIALLVVEGDKGLAGAFNANVNRGVASL
                     LAEKKEWESVVVMPIGKKAFDYWKRRTNPLAPKSYPGIFARVEYAQAKEIADWLTAEF
                     VEGRIDAAYVVFNEFRSVISQIVRRERLLPISRSEASLTEAAASGAPPTDYVYEPDPA
                     TILAKLLPRHLEFSIFRILLESAAAEHGARMTAMDSATRNAGDMIDSLTLTYNRARQA
                     RITKELIEIVSGAAAQNE"
     gene            1992..3440
                     /gene="atpD"
                     /locus_tag="VF958_11840"
     CDS             1992..3440
                     /gene="atpD"
                     /locus_tag="VF958_11840"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011525129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HEX9149844.1"
                     /translation="MAEQKIGRVVAVIGPVLDVEFPDGHMPDILNAIVVQDEGKDTGV
                     AIDVIAEVAQHLGENRVRCISMKPTDGMVRGMKAVDTGAPISMPVGEETLGRILNVVG
                     EPVDGLGPVKAKERWPIHREPPRYEDQSTEVEMFETGIKVIDLLEPYSKGGKTGLFGG
                     AGVGKTVIVLELINNVAIHHGGYSVFSGVGERTREGNDLYLEMTQSGVITPGDPKKSK
                     AALIYGQMTEPPGARLRVGLSGLTVAEYFRDVEGKDVILFIDNIFRFTQAGSEVSALL
                     GRMPSAVGYQPNLATEMGELQERITSTKKGSVTSVQAIYVPADDLTDPAPATAFAHLD
                     ATTVLSRQIAELGIYPAVDPLSSTSKILTPRIVGEEHYSTARAVQAVLQKYKDLQDII
                     AILGIDELSDDDKLTVARARKIQKFLSQPFFVAAQFTGLEGRYVKIADTIKGFREIVE
                     GKCDDIPEQAFYMVGTIEEAREKAAKIAKEAA"
     gene            3567..4238
                     /locus_tag="VF958_11845"
     CDS             3567..4238
                     /locus_tag="VF958_11845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013030695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HEX9149845.1"
                     /translation="MFIGHYAVGFAAKRVAPRTSLGLLLGSAVLLDLMWPIFLLLGWE
                     EVRVTRGPTPFLNLDFVSYPISHSLVASIGWALAAGGVYWVATRYRPGALWIAVGVVS
                     HWLLDFVTHRPDLALWPGGPRVGLGLWNSTLATIAVEGVFFAAGLWLYLSFTEPRDRN
                     GRRGLLAFVVFTIAIYASSIASPPPPNARAVAWVGLASWLMPVWAWWFDKHRSVEIGL
                     ARERG"
     gene            4253..5047
                     /locus_tag="VF958_11850"
     CDS             4253..5047
                     /locus_tag="VF958_11850"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX9149846.1"
                     /translation="MDLRIAGRVAMVAASSKGIGRGVAMSLLAEGCRVSICARTAAEI
                     EATREEMAAAAPITEVFGSRCDVSREEDLAAWHAATVERFGKVDILVTNTGGPPAARF
                     LDLTEEAWREGIEGVLMNVVRLSRLVLPDMRERRWGRIIHLTSLAAKQPMELLTVSST
                     LRAGISALTKTMSNEFSRDRVLVNAVMPGHILTARQKELNEIRSREQGIPVDEYSARV
                     VATIPIGRHGRPEEIGDVVTFLASERASYLTGATIQVDGGVIQSTF"
     gene            5091..5489
                     /gene="atpC"
                     /locus_tag="VF958_11855"
     CDS             5091..5489
                     /gene="atpC"
                     /locus_tag="VF958_11855"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit epsilon"
                     /protein_id="HEX9149847.1"
                     /translation="MKLKLQVVTPTKTVVATEADSVELPGELGYLGILPGHTPLITVL
                     KTGVLTYRNGGEDRSLAISAGFAEIANDTVTVLADGADEPREVDVAASERERSLAEEE
                     LKTAGRETLDEIRSRLELAQARLAIARRGR"
     gene            5625..6398
                     /locus_tag="VF958_11860"
     CDS             5625..6398
                     /locus_tag="VF958_11860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019460959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEX9149848.1"
                     /translation="MPEIAVVIPARDEADNVGPLLADVRAALDGRVDYEVVFVDDGSE
                     DATPAILHDASQAFPRLRWVRHRSPCGQSAAIWTGVRLARAPWIVTLDGDGQNDPADI
                     PTLLEARAARGADANLLVNGRRARRRDSWSKRVSSRIANAVRRRVLGDDTADTGCGLK
                     LFPREAFLRLPFFDHMHRFLPALVRRGGGEVVSVPVRHRERRHGRSHYGVHNRLWTGI
                     VDMLGVFWLQRRSKLPVLAGPASAAAGERAENGPSKEST"
     gene            6395..6691
                     /locus_tag="VF958_11865"
     CDS             6395..6691
                     /locus_tag="VF958_11865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019141526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-A-disaccharide synthase N-terminal
                     domain-containing protein"
                     /protein_id="HEX9149849.1"
                     /translation="MKIPPFWLIVGFVGQLLFSMRFLLQWVQSERAKKSVIPVAFWYF
                     SLGGSVVLLAYAVHRQDPVFILGQAAGIVIYLRNLYLISRERQAGDGSAPAASA"
     gene            6688..>7159
                     /locus_tag="VF958_11870"
     CDS             6688..>7159
                     /locus_tag="VF958_11870"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013820282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HEX9149850.1"
                     /translation="MSPAARRGLALVVLWAAVVLGGLMTRPAWPIDETRYLAVAWEMW
                     NHGESLVPHLNGAPYSDKPPLLFWLIEAGWRVFGVHEWWGRVVPSLFALASLFLTARL
                     ARRLWPDRPEIAAAAPFLLVATLFWSFFTTVLLFDMLLVFFTVVGVLGLVRASRG"
BASE COUNT         1157 a         2401 c         2419 g         1182 t
ORIGIN      
        1 atcgacgcga tgatcccgat cggccgcggc cagcgcgagc tcgtcatcgg cgaccgccag
       61 accgggaaga cggcggtcgc gctcgacacg atcatcaacc agaaggggaa aggcctgatc
      121 tgcgtctacg tcgtcatcgg ccagaagaag tcgacggtcg cgcaggtcgt caagaccctc
      181 gaggaatacg gcgcgatgga gcacaccatc gtcgtctccg cgaccgcgtc cgatcccgcg
      241 ccgatgcagt tcatcgcgcc ctatgccggt tgcgcgatgg gcgagtattt cctctacaac
      301 aagcagcacg ccctgacgat ctacgacgac ctctccaagc acgcggcggc gtatcgcgag
      361 atctcgctgc tccttcggcg gccgccgggg cgcgaggcat atcccggcga cgtgttctac
      421 ctccactcgc gccttctcga gcgcgcggcg aaactctccg acgagcgcgg cggcggctcg
      481 ctgacgtcgc ttccgttcat cgagacccag gccggcgacg tctccgctta cattccgacg
      541 aacgtcatct cgatcaccga cggccagatc tatctcgagt ccgacctctt ctattcgggc
      601 gtgcgtccgg cggtgaacgt cggcctctcg gtgtcgcgcg tcggcggcaa cgcgcagatc
      661 aaggcgatga aacaggtcgc cggaactctc cgtctggacc tcgcgcagtt ccgggagctc
      721 gcagcgttcg cccagttcgg ctccgacctg gacaagacga cccaggccca gctcgcccgg
      781 ggccagcggc tggtcgagat cctgaagcag gcgcagttcg cgccgatgga tgtggagctg
      841 cagatcgtct cgatctacgc gggcacgaaa ggattcctcg actcgctgcg cgttgatgcg
      901 gtcctgccgt tcgaagccgg gctccacgat ttcgtgcggc gcgagcactc cgaggtgctg
      961 tccgcgatcg tcaacacggg caagctcgaa aaggcgaccg aagaggccct cgccgccgcg
     1021 atcgcggcgt tcaaggaccg gttcgtgcgc gagaacgcgg acgcggtcgc cgcgtaggac
     1081 ttcggactca acgtggcctc tctcatcgac atccggcgac gcttgaaatc cgtccgcaac
     1141 atgcggcaga tcacgcgcgc catgaagatg gtgtcggccg cccgactgcg ccgggcgcag
     1201 gaccgcgcga tcgcggcccg gccgtacgcg aaggccctgc gcgaagtgct ggcgtcggta
     1261 tcgtcgcgcg tgaaggaggc gaagcacccg ctcctcggca cgcgggagga gcgccagatc
     1321 gcactcctcg tcgtcgaggg agacaagggc ctcgccggcg cgttcaatgc gaacgtgaac
     1381 cgcggcgtcg cttcgctgct cgccgaaaag aaggagtggg agtcggtcgt cgtgatgccg
     1441 atcggcaaga aggcgttcga ctactggaag cgccggacca acccgctcgc gccgaagtcg
     1501 tatccgggca tcttcgcccg ggtcgagtac gcgcaggcga aagagatcgc cgactggctg
     1561 acggcggagt tcgtcgaggg acgaatcgac gcggcgtacg tcgtgttcaa cgagttccgc
     1621 tccgtcatct ctcagatcgt ccggcgcgag cggctgctgc cgatctcgcg atccgaggcg
     1681 tcgctcaccg aggcggcggc ttccggcgca ccgccgacgg actacgtgta cgagccggat
     1741 cccgcgacca tcctcgcgaa gctcctcccg cggcacctcg agttctcgat cttccggatc
     1801 ctgctcgaat cggccgcggc ggaacacggc gcgcgcatga ccgcgatgga ttcggcgacc
     1861 cgcaacgcgg gcgacatgat cgattcgctg accctcacct ataaccgcgc gcggcaggcc
     1921 cggatcacaa aagagctgat cgagatcgtc tcgggcgccg cggcgcagaa cgaatgaccc
     1981 agggaggaaa aatggccgag cagaagatcg gacgcgtggt tgccgtcatc gggccggtgc
     2041 tcgacgtcga gtttccggat ggccacatgc ccgacatcct gaacgcgatc gtcgtccagg
     2101 acgaggggaa ggacaccggc gtcgcgatcg acgtcattgc cgaggtcgcc cagcacctcg
     2161 gcgagaaccg ggtccggtgc atctcgatga agccgaccga cgggatggtc cgcggcatga
     2221 aggcggtcga caccggcgcg ccgatcagca tgcccgtggg cgaagagact ctcgggcgga
     2281 tcctgaacgt ggtcggtgag ccggtcgacg ggctcggccc ggtcaaggcg aaggaacgct
     2341 ggccgatcca ccgcgagcca ccgcgctacg aggaccagtc gaccgaggtc gagatgttcg
     2401 agacgggcat caaggtcatc gatctgctcg agccctactc gaagggggga aagacaggcc
     2461 tcttcggcgg cgccggcgtc ggcaagaccg tcatcgtcct cgagctgatc aacaacgtcg
     2521 cgatccacca cggcggctac tcggtcttct ccggcgtcgg cgagcggacc cgtgaaggaa
     2581 acgacctcta cctcgagatg acgcagtccg gtgtcatcac acccggcgac ccgaagaagt
     2641 cgaaggcggc gctgatctac ggacagatga ccgagccccc gggcgcgcgc ctgcgcgtcg
     2701 ggctgtcggg cctgacggtc gccgagtact tccgcgacgt cgagggcaag gacgtgattc
     2761 tcttcatcga caacatcttc cgcttcaccc aggccggttc cgaggtctcc gcgctgctgg
     2821 gacgcatgcc gtcggcggtc ggctaccagc cgaacctcgc gacggaaatg ggggagctcc
     2881 aggagcgcat cacgtcgacg aagaagggct cggtgacgtc cgtccaggcg atctacgttc
     2941 cggccgacga cctgaccgat cccgcgccgg cgacggcgtt cgcgcatctc gacgcgacga
     3001 cggtcctctc gcggcagatc gcggagctcg gaatctatcc ggcggtcgac ccgctctcct
     3061 cgacctcgaa gatcctgacg ccgcggatcg tcggcgaaga gcactactcg acggctcgcg
     3121 ccgtccaggc ggtcctgcaa aagtacaagg accttcagga catcattgcg atcctcggca
     3181 tcgacgagct ctccgacgac gacaagctca cggttgcccg cgcgcgcaag atccagaagt
     3241 ttctctcgca gccgttcttc gtcgccgcgc agttcaccgg cctcgagggc cgctacgtga
     3301 agatcgccga caccatcaag gggttccggg aaatcgtcga gggcaaatgc gacgacatcc
     3361 cggagcaggc gttctacatg gtcgggacga tcgaggaagc gcgggagaag gccgcgaaga
     3421 tcgcgaaaga ggcggcgtag cgaggggtca ggtattcacg agtcgccgac gtcggtcgcc
     3481 gtgccgaact agtcttgccc cttcgccgcg cgaagtggca agtggagacc tgacccccga
     3541 agtggcaagt ggagacctga ccccacatgt tcatcggaca ctatgcggtc ggattcgccg
     3601 cgaagcgcgt ggcgccgagg acgtcgctcg gcctcctgct cggttcggcg gtgctgctcg
     3661 acctgatgtg gccgattttt ctccttctcg ggtgggaaga ggtgcgcgtg acccgcgggc
     3721 cgacgccgtt cctgaacctg gatttcgtga gctacccgat ctcccacagc ctggtcgcgt
     3781 ccatcggttg ggcgctcgct gccggcgggg tctactgggt cgcgacccgc tatcgtccgg
     3841 gcgcgctctg gatcgcggtc ggcgtcgtca gccactggct cctcgacttc gtgacccacc
     3901 gtccggacct ggcgctctgg cccggcgggc cgcgcgtggg gctgggtctc tggaactcga
     3961 cgctggcgac gatcgcggtc gagggagtgt tcttcgcagc cggactgtgg ctctacttgt
     4021 cgttcaccga gccgcgagac cgaaacgggc gccggggcct cctcgcgttc gtcgtgttca
     4081 cgatcgcgat ctacgcgtcg tcgatcgcga gtcccccccc gccgaacgcg cgcgcggttg
     4141 cgtgggtcgg cctcgcgagc tggctgatgc cggtgtgggc ttggtggttc gacaagcatc
     4201 gctcggtcga aatcggtctc gcgcgggaac gcggctgaag gagggaacag ggatggatct
     4261 gcgaatcgcc ggccgcgtcg cgatggtcgc cgcctcgagc aaggggatcg ggcggggcgt
     4321 ggcgatgtcg ctcttggccg aggggtgccg cgtctcgatc tgcgcgcgca cggccgccga
     4381 gatcgaggcc acacgcgagg agatggcggc ggctgccccg atcacggagg tcttcgggtc
     4441 ccgctgcgac gtgtcccgcg aggaggacct cgccgcctgg catgcagcga ccgtggagcg
     4501 ctttggcaag gtcgacatcc tcgtcaccaa tacgggaggc ccaccggccg cgcgattcct
     4561 cgatctcacc gaagaagcgt ggcgcgaggg aatcgaaggc gtcctcatga acgtcgtgcg
     4621 tctttcgcgg ctcgttcttc cggacatgcg cgagcggcgc tggggccgga tcatccacct
     4681 gacctcgctc gccgcgaagc agccgatgga actgctgacg gtttcgagca cgttgcgcgc
     4741 cggaatctct gcgctcacga agacgatgtc gaacgagttc tcgcgcgacc gcgtcctcgt
     4801 caacgccgtg atgccgggcc acatcttgac ggcgcggcag aaggagctga acgagatccg
     4861 gtcgcgggag caggggatcc cggtcgacga gtattcggcg cgcgtggtcg cgacgattcc
     4921 gatcgggcga cacgggcggc ccgaggagat cggcgacgtg gtcacgttcc tcgcgagcga
     4981 gcgcgcgagc tatctcaccg gcgcgacgat ccaggtggac ggcggggtca tccagagcac
     5041 gttctagctg cgggaaacgg gaaacggaaa acgggaagcg agaatgaacg atgaaactca
     5101 aacttcaggt cgtcacgccc acgaagacgg tcgtcgcgac ggaggcggac tccgtcgagc
     5161 ttcccgggga gctcggctat ctcggaatcc tgccggggca cacgcccttg atcacggtcc
     5221 tgaagaccgg cgtgctgacg tacaggaacg gcggcgagga tcgctcgctc gcgatctcgg
     5281 cgggattcgc ggagatcgcg aacgacaccg tcaccgtcct cgccgacggc gccgacgagc
     5341 cgcgcgaggt cgacgtcgct gcctcggaac gggaacgctc cctcgcggag gaggagctga
     5401 agaccgcggg tcgtgaaacg ctcgatgaga tccgcagccg gctcgagctc gcgcaggcgc
     5461 gcctcgcgat cgcccggcgc ggccgctaga cgcgggagcc acgaccgcct ttcacctccc
     5521 gttcagggtc gagacttact attaggccgg tcatgttctc gccggaggat tccgaaccga
     5581 agaacgttcg agcagccgtt ccgtcggggc ggccgctccg atccgtgccg gagatcgccg
     5641 tggtcattcc ggcccgggac gaagccgaca acgtcggtcc gcttctcgcg gacgttcgtg
     5701 cggcactcga cgggcgcgtc gactacgaag tcgtcttcgt tgacgacggg agcgaggacg
     5761 ctacgcctgc gatcctccac gacgcctcac aggcgttccc gaggctccgc tgggtgcgcc
     5821 accgcagccc gtgcgggcag agcgccgcga tctggaccgg cgtgcgcctc gcgcgcgctc
     5881 cctggatcgt gaccctggac ggcgacggcc agaacgatcc cgccgacatt ccgactctcc
     5941 tcgaagcgcg cgcggcgcgg ggcgccgatg cgaacctgct cgtgaacggt cggcgcgcgc
     6001 ggcggcgcga ctcctggtcg aagcgcgtct cctcgaggat cgcgaacgcg gttcgccgcc
     6061 gcgtgctcgg cgacgacacc gcggacaccg gctgtggcct gaagctcttc ccgcgagagg
     6121 cgttcctgcg tctccctttt ttcgaccaca tgcatcgctt cctgcccgcg ctcgtgcggc
     6181 gcggtggcgg cgaggtcgtg tccgtcccgg tccggcaccg cgagcgccgg cacggccgat
     6241 ctcactacgg ggttcacaac cgcctgtgga ccggcattgt cgacatgctc ggcgtgttct
     6301 ggctgcagcg ccggtcgaag ctgccggtgc tcgcggggcc ggcttcggcc gcggctggtg
     6361 agcgcgcgga gaacggtcct tccaaggagt cgacatgaag atccccccgt tctggctgat
     6421 cgttgggttc gtgggccaac tgctcttctc gatgcgcttt ctcctgcagt gggtgcagag
     6481 cgagcgcgcg aagaagagcg tcattcccgt cgcgttctgg tatttcagcc tcgggggctc
     6541 agtcgtactc ctcgcgtacg ccgtccatcg ccaggacccc gtcttcatcc tcggccaggc
     6601 ggcggggatc gtcatctacc tgcgcaacct ctacctcatc tcccgcgagc ggcaggcggg
     6661 agacggctcc gcgcccgcag cgagcgcatg agtccagccg cgcggcgggg gctcgccctc
     6721 gtcgtcctct gggcagcggt cgtcctcggc ggtctcatga cccggcccgc ctggccgatc
     6781 gacgagactc gatatctcgc cgtcgcgtgg gagatgtgga accacggaga gtctctcgtc
     6841 cctcacctga acggggcgcc ctacagcgac aagcctccgc tgctcttctg gctgatcgaa
     6901 gcgggatggc gggtattcgg agtccacgag tggtggggtc gtgtcgtgcc ctccctcttc
     6961 gcgctcgcga gcctgtttct gaccgcgcgg ctcgcccgcc ggctgtggcc cgaccgtccg
     7021 gaaatcgccg ccgcggcgcc gttccttctc gtcgcgacgc tcttctggtc gttcttcacg
     7081 accgtgcttc tcttcgacat gctgctcgtg tttttcacgg tcgtcggcgt gctcggcctc
     7141 gtccgggcct cccgcggtg
//
LOCUS       DASWAV010000569         1843 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U2665
            k141_154108, whole genome shotgun sequence.
ACCESSION   DASWAV010000569 DASWAV010000000
VERSION     DASWAV010000569.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960316
            Sequence Read Archive: SRR10260007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 1843)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1843)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:47:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,929
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1843
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_154108"
                     /isolate="SMAG_U2665"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.36444 N 132.31889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..554
                     /locus_tag="VF958_11875"
     CDS             <1..554
                     /locus_tag="VF958_11875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9149851.1"
                     /translation="QVFRGESENLFEAPSKNDLAYLGQYRVYRDIGDDHNVEVGATWG
                     RGHNGITNDSETTLENIHLVYRWRPLAGTPYRTFILRSELLRSRREQEDGRQTARGFF
                     VGGDYRFAQRWWAGGRYEFSDHADDSSLRDRGGAVTLTFWPSEFSQIRGELRQRKYAG
                     GIKANEAFLQLQFSIGAHGAHPF"
     gene            565..1479
                     /locus_tag="VF958_11880"
     CDS             565..1479
                     /locus_tag="VF958_11880"
                     /inference="COORDINATES: protein motif:HMM:NF013465.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HEX9149852.1"
                     /translation="MKKLLSLASLLFLVAVAPALGAVKVVTSLQDFASIADAIGGNRV
                     ETFAIAKGYQDPHFVDPKPSFVLKLNRADLLIAAGLELEIGYLPPLIDQSRNEKIHPG
                     NRGYLDASIGCDILNRPAGVVTRAMGDVHPFGNPHYWTDPDNGRVIARSVAAKLSELD
                     PAGRGTYQQNLAAFEAKLTQKGNEWKAKMAQYAGVKVVTFHDSWPNFAKHFNIAVRGH
                     IEPKPGIPPSPSHTLEIINLMKAERIPVIIVEPYFDTKTPNFIASKTGARVLRFYPSV
                     GGAPQITDYFSLFDTNIDTLVNALKQSR"
     gene            1476..>1843
                     /locus_tag="VF958_11885"
     CDS             1476..>1843
                     /locus_tag="VF958_11885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011682602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HEX9149853.1"
                     /translation="MSIFQILLPAFVASLILTGIHAYLGVHVVERGVIFVDLSLAQIA
                     ALGTTVAYLAGYDLHSSTAYLFSLGFTFLGAAIFAMSRVHRKTRIPQEAIIGIVYAVS
                     AAVAILVMSKATQETEHLKE"
BASE COUNT          346 a          597 c          560 g          340 t
ORIGIN      
        1 tccaggtctt ccgcggtgaa tccgagaatc tcttcgaggc accttcgaag aacgatctcg
       61 cgtacctggg gcagtaccgt gtctatcgcg acatcggcga cgaccacaat gtcgaggtcg
      121 gcgcaacatg ggggcgtggg cacaacggca tcaccaacga ctccgagacc actctggaga
      181 acatccacct tgtgtatcgg tggaggccgc tcgcagggac gccctaccgg acgttcatcc
      241 tgaggagcga gctcctgcgc agccgccggg agcaggagga cgggaggcag acggcccgtg
      301 gcttcttcgt cggcggcgac taccgcttcg cgcagcgttg gtgggccggc gggcggtacg
      361 agttctcgga ccacgccgac gactcaagcc tgcgggaccg cggcggagcc gtcacgctga
      421 ctttctggcc gagcgagttc tcgcagatcc gcggcgagct tcgccagcgg aagtacgccg
      481 gaggaatcaa ggcgaacgaa gctttcctcc aactccaatt ctcgatcggc gcccacggcg
      541 cccatccgtt ctagtgagga caaggtgaaa aaacttctct cgctcgcctc gcttctcttt
      601 ctcgtcgcgg tcgcgcccgc attgggcgcg gtcaaggtcg tgacgagctt gcaggatttc
      661 gcgtcgatcg ccgacgcgat cggcggcaac cgcgtcgaga ccttcgcgat cgcgaagggc
      721 taccaggatc cccatttcgt cgacccgaaa ccctcgttcg ttctgaagct gaaccgcgct
      781 gacctgctga tcgcggcggg actcgagctc gagatcggat atctgccgcc cctgatcgat
      841 cagagccgca acgagaagat ccatccgggc aaccgcggct atctcgatgc atcgatcggg
      901 tgcgacatcc tgaatcggcc ggccggggtc gtcacgcgag ccatgggcga cgtacacccg
      961 ttcggcaacc cccactactg gaccgatccg gacaacggcc gcgtcatcgc gcggtcggtc
     1021 gcggcgaagc tctcggagct cgacccggcg gggcgcggga catatcaaca gaacctcgcc
     1081 gcgttcgaag cgaagctcac gcagaaaggc aacgagtgga aggcgaagat ggcgcagtac
     1141 gccggtgtca aggtcgtcac gttccatgac tcctggccga atttcgccaa gcacttcaac
     1201 atcgcggtgc ggggtcacat cgagccgaag cccggcattc caccgtcgcc atctcacacg
     1261 ctggaaatca tcaatctgat gaaggcggaa cggatccccg tcatcatcgt cgagccttac
     1321 ttcgacacga agactccgaa cttcatcgct tcgaagacgg gcgcgagggt cctccggttc
     1381 tatccctcgg tcggcggcgc gccgcagatc acggactact tttccctctt cgatacgaac
     1441 atcgatacgc tcgtgaacgc gctgaagcag tcccgatgag catctttcag attctgttgc
     1501 ccgcgtttgt cgcgagcttg atcttgacgg ggatccacgc ttatctcggc gttcacgtgg
     1561 tcgagcgagg ggtcatcttc gtcgatctct cgctcgccca gatcgccgcg ctcggcacaa
     1621 ccgtcgctta cctcgcggga tacgacctgc actcgagcac ggcgtatctc ttctccctcg
     1681 gcttcacgtt tctgggcgcg gcgatcttcg cgatgtcgcg cgtgcaccgg aagactcgga
     1741 tcccgcagga ggcgatcatc ggaatcgtct acgccgtatc cgcggcggtg gcgattcttg
     1801 tgatgtcgaa ggcgacccag gagaccgagc acctgaagga gat
//
LOCUS       DASWAV010000570         1841 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U2665
            k141_193753, whole genome shotgun sequence.
ACCESSION   DASWAV010000570 DASWAV010000000
VERSION     DASWAV010000570.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960316
            Sequence Read Archive: SRR10260007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 1841)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1841)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:47:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,929
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1841
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_193753"
                     /isolate="SMAG_U2665"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.36444 N 132.31889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..303)
                     /locus_tag="VF958_11890"
     CDS             complement(<1..303)
                     /locus_tag="VF958_11890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020719565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HEX9149854.1"
                     /translation="MTSIVIVESPAKARTLEKFLGKNFRVLASYGHVRDLPKKGLGVD
                     REHSYEPTYEVLHGKEKTLAELKRAAQKADVVYLAADPDREGEAISWHLKETLTPSA"
     gene            complement(451..1557)
                     /gene="dprA"
                     /locus_tag="VF958_11895"
     CDS             complement(451..1557)
                     /gene="dprA"
                     /locus_tag="VF958_11895"
                     /inference="COORDINATES: protein motif:HMM:TIGR00732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HEX9149855.1"
                     /translation="MVSIPLARRAAVSWARSRDLTPQRLWPVLRDRGGLDALLATPEA
                     DLADRLASRERARAVLAGPSDSAAERWAASVETSGIRIVTAFDAEYPRALLEIADPPF
                     LLFSVGRLEKFRLPAVAVVGSRSGTRYGRDVAARLSRELSLAGVTIVSGFARGVDVAA
                     HEAAREGPGGTVAVLGCGLDVDYPREHARLKTAMAGTDLLVSEYPPGAEPRPQNFPIR
                     NRIIAGIAAGVVVVEASRRSGSLITARLAADFGRDVFAVPGSVFSETSVGTHELLRDG
                     AILCRGAEDVLAELFPSIGAPGAAPRVTSSLASLSPDAARLSAALEREDALSAEELAQ
                     ALDLPAAVVLAALFELEGAGLVIAEGGRYSAIRR"
BASE COUNT          316 a          617 c          660 g          248 t
ORIGIN      
        1 cgcggacggc gtgagcgtct ccttcaggtg ccaggagatg gcctcgccct cgcggtcggg
       61 gtcggcagcg aggtacacga cgtccgcctt ctgggcggca cgcttcagtt ccgcgagcgt
      121 tttctccttg ccgtggagga cctcgtacgt gggctcgtag gagtgctcgc ggtccacccc
      181 caggcccttc ttcggaaggt cgcggacgtg gccgtaggac gccagcacac ggaaattctt
      241 cccgagaaac ttctcgaggg tgcgcgcctt cgccggcgac tcgacgatca cgatgctggt
      301 catgacaggg tgcgggacgg tgccacaacc gaccataaag cgcaatcgac tcgcgacgcg
      361 cgaagcggtt tcgcgggtcg ccccgagcga tgcgaaggac ctgttgaggc gtgtcccgat
      421 gacgacaaca aggccgtatt cccgtgaccc ctaacgcctg atcgccgagt agcgtccgcc
      481 ctcggcgatg acgagccccg cgccctcgag ctcgaacagc gcggcgagga ccaccgccgc
      541 gggaaggtcg agcgcctgcg ccagctcctc ggcagagagc gcgtcttcgc gctcgagcgc
      601 cgcggagagg cgagctgcgt cgggcgagag gctcgcgaga gatgaggtga cgcgcggcgc
      661 ggcgcccgga gccccgatcg acggaaagag ctcggcgagg acgtcctcgg ccccgcggca
      721 caggattgcg ccgtcgcgga gcagctcgtg ggtcccgacc gacgtttccg agaagacgct
      781 gcccggaacg gcgaacacgt cgcggccgaa gtcggccgcg agccgcgcgg tgatgaggga
      841 tccgctccgt cgcgacgcct cgacgacgac gacgccggcg gcgatcccgg cgatgatgcg
      901 gttgcggatc ggaaagttct gggggcgcgg ctcggccccc ggcgggtact ccgaaacgag
      961 gaggtcggtc cccgccatgg cggtcttcaa gcgcgcgtgc tctcgcggat agtcgacgtc
     1021 gaggccgcag ccgagcacgg cgacggttcc gccgggaccc tcgcgcgcag cctcgtgggc
     1081 cgcgacgtcc acgccgcgcg cgaaccccga aacgatcgtc acgcccgcga gcgagagctc
     1141 gcgcgagagc cgcgccgcca cgtcccgtcc gtagcgcgtt ccgctccggg acccgacgac
     1201 cgcgaccgcc ggaagccgaa acttttccag ccggccaacg gagaagagga ggaagggagg
     1261 gtcggcgatc tcgagaagcg cccgggggta ctcggcatcg aatgccgtca cgatccggat
     1321 tcccgaggtc tcgacggacg ccgcccaccg ctcggcggcg gagtccgacg gtccggcgag
     1381 gaccgcgcgt gcgcgttccc gggaggccaa ccgatcggcg aggtccgctt ccggcgtcgc
     1441 caggagagca tcgagaccac cccgatcccg caggaccggc cagagccgct gcggggtcag
     1501 gtcccgtgat cgggcccagg agaccgcggc tcttcgggca agaggaatgg aaaccatcct
     1561 taacaatatt ttcagatgag attgaggcac ccgatattcg cggcgcaagg agtctcccga
     1621 ggtaatcgag ggaggagcct tgcgccgcgg gagcgcgagc gcagcgaggt atcaggcgct
     1681 cggtcgaggc cgacctgccg agccgagcgc cggtggcgga gccagataac gagcaagcga
     1741 gcgagacgaa cctataatag ccgcccccct gtgggggttc agaggtttgg gtgaaaaagc
     1801 tcgcgctcgt tctgacagcc gtcctgatca ccgccgggtg c
//
LOCUS       DASWAV010000571         2364 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U2665
            k141_71010, whole genome shotgun sequence.
ACCESSION   DASWAV010000571 DASWAV010000000
VERSION     DASWAV010000571.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960316
            Sequence Read Archive: SRR10260007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2364)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2364)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:47:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,929
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2364
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_71010"
                     /isolate="SMAG_U2665"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.36444 N 132.31889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..883
                     /locus_tag="VF958_11900"
     CDS             <1..883
                     /locus_tag="VF958_11900"
                     /inference="COORDINATES: protein motif:HMM:NF014679.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005504 - fatty acid binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HEX9149856.1"
                     /translation="RFDKNRAERVGIVTDSTCDLPDGMLEDLNVSVVPVRVYFGSENY
                     LDKVTLSPAEFYARFAVTDEPPKTSQPPPADFTQVYRNVASHAAKVVSIHLSKAVSGT
                     YQAAIVGSRPIENTQFEHVNSRNASVGLGLVVRAAAEAAAAGKSFKEVVQIARDATNR
                     VTVYIAVPTLEHLIRGGRVPPMKGWIAKLFGLLPVLTLTKSGTAEAVARARGFEPALQ
                     KMMRLLFSAARSGNGSGPSRERRFGVAHCDASELAERLAREIRERYPESDVMTVECGP
                     ALGAHGGPGAVAVAVLT"
     gene            880..1410
                     /locus_tag="VF958_11905"
     CDS             880..1410
                     /locus_tag="VF958_11905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020706932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 domain-containing protein"
                     /protein_id="HEX9149857.1"
                     /translation="MNRVWTVGHSNHSVERFVEILKAHGIARILDVRRFPMSRKWPHF
                     NADSLAASLPASEIEYVGIPELGGRRKPRPDSPHTAWRADQFRGYADFMDTPDFAANL
                     ERAMAYARERPTALMCAEAVPWRCHRSLLSDALSARGWDVSEILSEKEARPHKLPGFA
                     RVEGTRVVYDGGELPL"
     gene            complement(1452..1874)
                     /locus_tag="VF958_11910"
     CDS             complement(1452..1874)
                     /locus_tag="VF958_11910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9149858.1"
                     /translation="MRSDAIAALLFAAALGCTHAAPPPDRTGTPAAAPGERFRLRKGD
                     SLRITGTHARITFEKVVSDNRCPVDVACVIAGEARVLFRLDELEKSPLGFELDTGKNP
                     NATVNGYAVALLLVSPAPRSTAQIDPQSYVVELTVTPR"
     gene            complement(1864..>2364)
                     /locus_tag="VF958_11915"
     CDS             complement(1864..>2364)
                     /locus_tag="VF958_11915"
                     /inference="COORDINATES: protein motif:HMM:NF014609.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis isomerase"
                     /protein_id="HEX9149859.1"
                     /translation="TDEVASVAEALGLAEEEIQATNQPVRVASTGLPVLIVPVATLAA
                     LFAARPRYGLLRDLLEGRDATLVYAFTKETKEARSAAHARAFDTVGSVEVPGAGAAAG
                     ALAAYLVSHNAVTVQPVTVLPIEQGHAVNRPSTIYTEVHLDSGEITRVVMGARVVRVG
                     EGRLEI"
BASE COUNT          378 a          804 c          805 g          377 t
ORIGIN      
        1 ccgcttcgac aagaaccgcg ccgagcgagt ggggatcgtc accgattcga cgtgcgacct
       61 tccggatggg atgctcgagg atctcaatgt ctccgtcgtg ccggtgcggg tctacttcgg
      121 ctcggagaac tacctcgaca aggtgacgct gtcgcccgcc gagttctacg cgaggttcgc
      181 ggtaaccgac gaacccccga agacttcgca gcctccgccc gcggacttca cgcaggtcta
      241 tcgcaacgtc gcgtcgcacg cggcgaaagt ggtttccata cacctgtcca aggcggtctc
      301 ggggacgtac caggcggcga tcgtgggctc gcggccgatc gaaaacacgc agttcgagca
      361 cgtcaacagc cggaacgcgt cggtcgggct cggcctcgtc gtccgcgcgg cggcggaagc
      421 cgcggctgcg ggcaagtcgt tcaaggaggt ggttcagatc gcgcgcgacg cgacgaaccg
      481 cgtgacggtc tacatcgcgg tgccgacgct cgaacacttg atccgcggcg ggcgcgttcc
      541 gccgatgaag ggctggatcg cgaagctctt cggcctcctg cctgtgctga ctctcaccaa
      601 gagcgggacg gccgaggcgg tcgcgcgggc acgaggtttc gagccggccc tccagaaaat
      661 gatgagactc ctcttctccg ccgcccggtc cggcaacggg tccggaccct cgcgcgagcg
      721 gcggttcggc gttgcccatt gcgacgcgtc ggaacttgct gagcggcttg cgcgcgagat
      781 ccgtgagcgt tacccggaga gcgacgtcat gaccgtcgag tgcgggcccg ctctcggtgc
      841 ccacggggga ccgggggcgg tcgcagtcgc cgtcctcact tgaaccgggt ctggacggtc
      901 ggacactcca accactccgt cgaacgattc gtcgaaatcc tgaaggcgca cgggatcgcg
      961 cggatcctcg acgtgcggcg attcccgatg tcgcgcaagt ggccgcactt caacgccgac
     1021 agcctcgctg cgagtcttcc ggcctccgaa atcgaatacg tcggcattcc ggagctgggt
     1081 ggacgacgca agccccgtcc cgactctccg cacaccgcct ggcgcgccga ccagttccgg
     1141 ggctacgcgg acttcatgga caccccggac ttcgccgcga acctcgagcg ggccatggca
     1201 tacgcccgcg agcggccgac ggccttgatg tgcgcggaag cggtaccgtg gcgctgccac
     1261 cgctcgcttc tctcggacgc actctccgcc cgcggctggg acgtctctga gatcctgagc
     1321 gagaaggagg ctcgcccgca caagctcccg ggcttcgcgc gggtcgaagg aacacgggtc
     1381 gtctacgacg gcggcgagct gcccctctga tgtcgcgggg acagaccatg cggcctgcac
     1441 ccgcctcctt ctcaccgcgg ggtgaccgtc agctcgacga cgtaactctg cggatcgatc
     1501 tgggcggtcg aacgcggagc aggggagacg aggaggagcg ccaccgcgta gccgttcacg
     1561 gtggcgttcg gattcttgcc ggtgtcgagc tcgaatccca ggggggactt ttcgagttcg
     1621 tccagccgga agaggacccg tgcctcgccg gcgatgacgc acgcgacgtc gaccgggcag
     1681 cggttgtccg aaacgacttt ttcgaacgtg atccgtgcgt gggtcccagt gattctcagg
     1741 gaatcgccct tccggagtct gaaccgctcg ccgggagctg ccgcaggagt cccggttcga
     1801 tcgggaggcg gcgcggcgtg cgtgcaaccg agcgccgccg cgaagagcag ggccgcgatc
     1861 gcgtcagatc tcaagccgcc cctctccgac gcgcacgaca cgtgcgccca tgaccacgcg
     1921 ggtgatctcc ccgctgtcga ggtgaacttc cgtgtagatc gtcgacggtc ggttcacggc
     1981 gtgaccctgt tcgatcggga ggaccgtcac cggctgcacc gtcaccgcgt tgtgagaaac
     2041 gagatacgcc gcgagggcgc cggccgcggc tccggcgccg ggcacttcga ccgagccgac
     2101 cgtgtcgaac gcgcgggcat gagcggccga gcgggcctcc ttggtctcct tcgtgaacgc
     2161 gtagacgagg gtggcatcgc gcccctcgag caggtcgcgc aggaggccat atcggggccg
     2221 cgccgcgaag agcgcggcga gcgtcgccac cgggacgatc aggaccggaa gcccggtcga
     2281 tgccacgcga accggctggt tcgtcgcctg gatctcttct tccgcgagac cgagcgcttc
     2341 cgccacactc gcaacctcgt ccgt
//
LOCUS       DASWAV010000572         1950 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U2665
            k141_232824, whole genome shotgun sequence.
ACCESSION   DASWAV010000572 DASWAV010000000
VERSION     DASWAV010000572.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960316
            Sequence Read Archive: SRR10260007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 1950)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1950)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:47:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,929
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1950
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_232824"
                     /isolate="SMAG_U2665"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.36444 N 132.31889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(76..903)
                     /locus_tag="VF958_11920"
     CDS             complement(76..903)
                     /locus_tag="VF958_11920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012224208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX9149860.1"
                     /translation="MSRPTGPRGSTTPVPKIRVENLRKSFQGKVVLDGIDLSVAEGES
                     VVIVGPSGTGKSVLLKHLIGLIRPDSGKVYVDGRDFWALGETERIGVRKKFGMSFQEG
                     ALFDSMSVFDNVAFPLRRSGRPPAEVRQRVRECLDMVHLPDVENKRPSELSGGMRRRV
                     GFARAIAHQPEILLFDEPNTGLDPIMTDVIDEVILELKERLDVTIVTITHHMESARKI
                     GDRVALIHGGRLLYQASPEEFLRSDDPAVRQFVEGRAEGPLTVEFDRKSESARLEGA"
     gene            complement(900..1667)
                     /locus_tag="VF958_11925"
     CDS             complement(900..1667)
                     /locus_tag="VF958_11925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018287938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX9149861.1"
                     /translation="MRFLEALGRMVLRLFEELGRFFQMLGRTAGWSIRPPYDVPELFR
                     QMVRVGVDSIPVVFLTTLFTGMVMALQTFNGFARFHAESLVGSVVALSLTRELSPVLT
                     ALMVTGRVGSAMAAELGTMRVTEQIDALTALGTEPVQYLVVPRVTASIAMMPLLVMLG
                     DAVGMYGGYLVAVQLMGANPVTYVENSFQFLEVSDVTSGIVKAAVFGLIFSLVACVRG
                     YYTEGGAEGVGRSTTRAVVSGSLAVLFADFFLTKLLF"
     gene            complement(1664..>1950)
                     /locus_tag="VF958_11930"
     CDS             complement(1664..>1950)
                     /locus_tag="VF958_11930"
                     /inference="COORDINATES: protein motif:HMM:NF013038.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="alanine racemase C-terminal domain-containing
                     protein"
                     /protein_id="HEX9149862.1"
                     /translation="VVPIGYHDGFRRSFSGRVRVLMKGNEAPVVGAVSMDLTLVDATG
                     LGVETGDRVVCLGAADGLRVTAWDLARAADTLPYEILCGIGARVPRAYAP"
BASE COUNT          371 a          660 c          618 g          301 t
ORIGIN      
        1 cctcgatctt ccagatgaag agcgcgacga ttgcgagaac aaccaggaaa aaagcgccga
       61 ccttcgccgc cgtattcatg cgccctccaa acgcgccgac tcgctcttcc ggtcgaactc
      121 taccgtgagc ggcccttccg cgcgcccctc gacgaactgc cggacagccg ggtcgtcgga
      181 acgcagaaat tcttcggggg aggcctggta gagcagtcgc cccccgtgaa tgagcgccac
      241 ccgatccccg atcttgcgcg cgctctccat gtgatgcgtg atcgtcacga tcgtgacgtc
      301 gagtcgctcc ttgagctcca ggatgacctc gtcgattacg tccgtcatga tcgggtcgag
      361 acccgtattt ggctcgtcga acaggagtat ctcgggctgg tgcgcgatcg cgcgcgcgaa
      421 accgacgcgc cgccgcatgc cgccggagag ctctgagggc ctcttgttct cgacgtccgg
      481 caggtggacc atgtcgaggc attcccgtac gcgctggcgc acctccgccg gcggccttcc
      541 cgatcgccgc agcgggaacg cgacattgtc gaaaaccgac atcgagtcga aaagcgctcc
      601 ctcctgaaac gacatcccga acttctttcg gacgccgatg cgttccgttt cgccgagcgc
      661 ccagaagtcc cggccgtcca cgtagacctt tccggaatcc ggccggatga ggccgatcag
      721 gtgcttcagg agcacgctct ttccggtccc ggagggcccg acgatgacga ccgactcccc
      781 ctccgcgacc gagaggtcga ttccgtccag gaccaccttg ccctggaaag acttgcgcag
      841 gttctcgact cggatcttcg gaacgggcgt cgtcgagcct ctcgggccgg tcgggcggct
      901 caaaacagca gcttcgtcag aaagaagtcg gcaaagagga cggcgaggct gcccgagacg
      961 acggcacggg tcgtcgagcg ccccacccct tccgcacccc cctcggtgta gtacccgcgg
     1021 acacacgcaa cgagcgagaa gatgagaccg aagacggcgg ccttgacgat ccccgacgtg
     1081 acgtcggaga cctccaggaa ctggaacgag ttttcgacgt acgtaaccgg gttcgctccc
     1141 atgagctgca cggcgacgag gtacccgccg tacattccaa ccgcatcgcc gagcatcacg
     1201 aggagcggca tcatcgcgat ggaggctgta acgcgcggta cgaccaggta ttgcacgggc
     1261 tccgtgccga gggccgtcag cgcgtcgatc tgctcggtca cccgcatggt gccgagctcg
     1321 gccgccatcg cgcttccgac gcgcccggtc accatgaggg ccgtgaggac gggtgagagc
     1381 tcccgggtca gcgagagcgc cacgacgctt ccgacgaggc tttctgcatg gaatcgagcg
     1441 aacccgttga aggtctggag cgccatgacc atgccggtga agagcgtcgt caggaagacg
     1501 accggaatgg agtcgacgcc gactcgcacc atctgccgga agagctcggg gacgtcatag
     1561 ggcgggcgaa tcgaccagcc cgccgtgcga ccgagcatct gaaagaaacg gccgagctcc
     1621 tcgaacagcc gcaggaccat ccggccgagt gcctcgagaa acctcacggc gcgtaggcgc
     1681 ggggaacccg cgctccgatt ccgcagagga tctcgtaggg cagcgtgtct gcggctcgcg
     1741 cgaggtccca cgccgtgacg cggagaccgt cggcggcgcc gaggcagacg acgcggtcgc
     1801 cagtctccac gccgagcccg gttgcgtcga cgagagtcag gtccatgctc actgcgccca
     1861 ccacgggcgc ttcgttcccc ttcatcaaga cgcgaacgcg ccccgagaag gagcgccgga
     1921 agccgtcatg atagccgatt ggaacgacgg
//
LOCUS       DASWAV010000573         4271 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U2665
            k141_671552, whole genome shotgun sequence.
ACCESSION   DASWAV010000573 DASWAV010000000
VERSION     DASWAV010000573.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960316
            Sequence Read Archive: SRR10260007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 4271)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4271)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:47:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,929
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4271
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_671552"
                     /isolate="SMAG_U2665"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.36444 N 132.31889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2512)
                     /locus_tag="VF958_11935"
     CDS             complement(<1..2512)
                     /locus_tag="VF958_11935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9149863.1"
                     /translation="MTIFRHLVCAVLLACGALPAGATAQQPADDVPVPGGPGAIRRLL
                     GLDPTRPRETFFLDLHEALLAGSGWNARWSQIDRRRAVVDFSEDLAALRRQFGRSFTI
                     SLSSEADRARASKLLSWLGVDVDRKGDEISTEIRDDVRAVRRRACFDAAVSMPVAALL
                     TKLRAGERVTVALTDETAPLPFSLDVWRELLDEPRLSSGDVFLELVKNVGGSRLLVTM
                     HALDAGTREGLRAIVRSGRGSVLWDRDVLDRLARFPGALALSGGEFVLPGGREADPIW
                     TDMFGVSPSQPGAFLRALYEKDSGRGAYFIEVLQQLPAPITRALLFGASGDGAGAVAR
                     IRKVYAAIEETRRSVDVSHRDPYDFAHLARFLRAGPSGDLALLPVDLDGEAFPKNEAE
                     LAGIIDRANRSDAPEDTLARLVRGEVGAGVDARRAARRFILVSSLLEGRPALEDRGVV
                     ALLLRGADRFASAYALLEDAPFQEPTLARRYLFAVDRLDRRKPSREEEVAAALFQAGS
                     EILACAYRAGSLDETATRALFSAFLDLPLFARSDLAPAKGERDVYGWLATKLLPALEK
                     GAAREPDSAPETNGEPSEDRSVDELLSRGLVGSPPAAVFEWNGGHYRFDPTTDELVRR
                     RGFREKQRLATLEDLAAIHRERDSLVAGAVRGDRTAVQAGAEALVDRLAVSEESGGVG
                     AREDERILEEYGRVREALDRLAKAPRAITAAQAEKDLSPVDAVVAERLLEALLGHVYA
                     AAAGDPEDLYYEDPDFVRRHSFRNVEKGGRTVESAFSPTALTSEATGGGARVSGSLFA
                     LADVLGLLHADQITYLPGAMIANENIRSGLIGPVRRMSP"
     gene            complement(2574..3875)
                     /locus_tag="VF958_11940"
     CDS             complement(2574..3875)
                     /locus_tag="VF958_11940"
                     /inference="COORDINATES: protein motif:HMM:NF013715.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nramp family divalent metal transporter"
                     /protein_id="HEX9149864.1"
                     /translation="MTRHEAVTPPSGPRTRSRPGPIRRFFSVLGPGVITGAADDDPSG
                     IATYSIAGAQFGTSLLWTAVLTWPLMAAVQMMCARIGMVTGHGLAGALRRKFPRPLLV
                     AAASALLVANTINIAADLAGMADAAEMLTGANSHLFVVLFGVGIAVAIIRFRYHQIAT
                     ILKWLALVLLAYVVTAVHVGPDWSSVLRTAFLPSWPRERAQWATLVAILGTTISPYLF
                     FWQASQEVEEEKAMGRRMLIRREGATSQEIATRKLDVGVGTFFSNLVMFFVILTTALT
                     LHRHGLTKLETSKEVAQALRPLAGRFAEVLYTAGIVGVGLLAIPTLAGSAAYAFAEVF
                     GWKQGLDRKWRNARYFYRVVAFSTAVGIALDFAEINPVRALYWTAIVNGFLAPFLLVG
                     ILLAATDSRLMRGQPSSRVGRVVVAVAAALMFGAAAGVLIF"
     gene            complement(3888..>4271)
                     /locus_tag="VF958_11945"
     CDS             complement(3888..>4271)
                     /locus_tag="VF958_11945"
                     /inference="COORDINATES: protein motif:HMM:NF013821.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin kinase"
                     /protein_id="HEX9149865.1"
                     /translation="AIDGLVAKGDKVGRRIGYPTINLEPENDLHPADGVYVTQIDIRS
                     FGRRFDCVTNIGRRPTVYEDYATVIETYVLDFSANVYGERVRLYFFERLREERRFPSV
                     TALSDQIGRDIEATRTYFSRRQGGP"
BASE COUNT          753 a         1451 c         1465 g          602 t
ORIGIN      
        1 ccggcgacat gcgccgcacc gggccgatca gaccggatcg gatgttctcg ttggcgatca
       61 tggcgccggg caagtaggtg atctggtcgg cgtggaggag cccgagcacg tccgcgagcg
      121 cgaaaagcga cccgctcacc cgcgcgcctc cgccggtggc ctcggacgtg agcgcggtcg
      181 gagagaacgc ggactccacg gttcgtccac ctttctccac gtttcggaac gaatgccgcc
      241 ggacgaagtc ggggtcctcg tagtagaggt cctcggggtc tcccgcggct gcggcataga
      301 cgtggccgag cagtgcctcg aggaggcgct cggcgacgac ggcgtctacg ggagagagat
      361 ccttctccgc ctgagcggcg gttatcgccc gaggggcttt cgcgaggcgg tcgagcgcct
      421 cgcggacgcg gccgtactcc tcgaggatcc gctcgtcctc gcgcgcgccg acgcctcccg
      481 actcctcgga aacggcgagc cggtcgacga gcgcctcggc gcccgcctgt accgcggtcc
      541 ggtctccgcg caccgcaccc gccacgagcg agtcccgctc gcgatggatg gccgccaggt
      601 cctcgagcgt ggcgagacgc tgcttctcgc gaaacccacg tctccgcacg agctcgtccg
      661 tcgtcggatc gaacctgtaa tgccccccgt tccactcgaa cactgccgcg ggaggagagc
      721 cgacgaggcc gcgcgagaga agctcgtcca cggagcgatc ctccgacggc tcgccgttcg
      781 tctccggcgc agagtcgggt tcgcgcgccg cacccttctc gagcgccggg agaagctttg
      841 tcgcgagcca tccgtagacg tccctctctc ccttcgccgg agcaaggtcc gaccgcgcaa
      901 agagcgggag gtcgagaaac gcggaaaaga gcgcacgggt ggccgtctcg tcgagcgagc
      961 ccgcgcggta cgcgcacgcg agaatctctg aacccgcctg gaacaacgcg gccgcgacct
     1021 cctcctctcg cgacggcttg cgtcgatcga ggcgatcgac ggcgaagaga tagcgccgcg
     1081 caagcgtcgg ctcctggaaa ggggcgtcct cgaggagcgc gtacgccgac gcgaaccggt
     1141 ccgcgccccg cagaaggagg gcgacgacgc cgcggtcctc gagggccggc cgcccctcga
     1201 gcaggctgga cacgagaatg aaacgccggg ccgcgcgccg ggcgtccaca ccggccccca
     1261 cttcgccccg cacgagacgg gcaagagtgt cctccggcgc gtcgctgcgg ttcgcccggt
     1321 cgatgattcc ggcgagctcc gcctcgttct tcggaaacgc ctccccgtcg agatccacgg
     1381 ggagcagagc gaggtcgccc gacgggccgg cgcgcaggaa acgcgcgagg tgagcaaagt
     1441 cgtacgggtc gcggtgcgag acgtccaccg accgacgcgt ctcctcgatc gcggcgtaga
     1501 cctttcggat tcgggcgacc gcgccggctc cgtcgccgga agcgccgaag agcagagcgc
     1561 gcgtgatcgg cgccgggagc tgctgcagga cctcgatgaa gtaggcgcct ctccccgagt
     1621 ccttctcgta caacgcgcgg agaaaggccc cgggctgcga gggcgacacg ccgaacatgt
     1681 ccgtccagat cggatcggct tcccggccgc cggggaggac gaattcccca ccggacaggg
     1741 cgagggcgcc cggaaagcgc gccaggcggt ccaggacgtc cctgtcccag agaaccgatc
     1801 cccgccccga ccgcacgatc gcgcgaagac cctcgcgggt cccggcgtcg agggcatgca
     1861 tcgtgacgag cagacgcgat ccaccaacgt tcttcaccag ctcgaggaac acatcgccgg
     1921 acgaaaggcg cggctcgtcg agcagctccc gccagacatc caggctgaag ggaagcggtg
     1981 cggtctcgtc cgtcagggcg accgtgacgc gctccccggc gcgaagtttc gtcagcagag
     2041 ccgcgaccgg catcgaaacg gccgcgtcga agcacgcccg ccggcggacg gcgcgcacgt
     2101 cgtctcggat ctcggtcgag atctcgtctc cttttcggtc aacgtcgacg cccagccagg
     2161 agagaagctt cgaggcgcgc gcgcgatccg cctcgctcga gagcgagatc gtgaacgacc
     2221 gcccgaattg cctccggagc gcggcaagat cttcgctgaa gtcgacgacg gcgcggcgcc
     2281 gatcgatctg cgaccaccgc gcgttccatc ccgagcccgc gaggagagcc tcgtgcaggt
     2341 cgagaaagaa ggtctcgcgc ggccgggtgg ggtcgaggcc gagaagccgc cggatcgccc
     2401 ccggcccgcc gggcaccggc acgtcgtcag cgggttgctg cgccgtcgcc ccggccggaa
     2461 gcgcgccgca agcgagcagc acggcgcaga cgaggtgccg gaaaatcgtc attgtttcgg
     2521 ggttcgcctc cgggtcgaga ccgggtttct cctgctcctg aacgacaatc taatcagaaa
     2581 atcaacactc cggccgccgc accgaacatg agcgcggccg ccaccgcgac gacgacccgt
     2641 ccgacccgcg agctcggttg gcctctcatc aaccgggagt cggtcgcggc caggaggatc
     2701 ccgacgagca gaaacggagc gaggaacccg ttgacgatgg ccgtccagta gagagcccgc
     2761 accggattga tctcggcgaa gtcgagggcg atgccgacgg ccgtcgagaa ggccaccact
     2821 ctgtagaagt agcgggcgtt tctccacttc cggtcgagcc cctgcttcca gccgaagacc
     2881 tcggcgaacg catatgccgc cgagccggca agggtgggta tcgccagcag cccgacgccg
     2941 acgattcccg cggtgtagag cacctcggcg aagcgccccg cgagcggccg aagcgcctgg
     3001 gcgacttcct tcgaagtctc gagcttcgtc aagccgtgcc ggtggagcgt caaggccgtg
     3061 gtcaggatga cgaagaacat cacgaggttc gaaaaaaagg tgccgacacc gacgtcgagc
     3121 ttccgggtcg cgatctcctg ggacgtggcg ccctcgcgac ggatcagcat tcgacgtccc
     3181 atcgcctttt cttcctcgac ctcctgagac gcctgccaga agaagagata cggagagatg
     3241 gtcgttccca ggatggcgac gagcgttgcc cactgtgcac gttcgcgcgg ccacgacggg
     3301 aggaaggccg tgcgcagaac cgaggaccag tcggggccga cgtggacggc ggtcacgacg
     3361 taggcgagca ggacgagtgc gagccacttc aggatggttg cgatctggtg ataccgaaaa
     3421 cggatgatcg ccacggcgat gccgaccccg aagagaacga cgaataggtg cgagttggcg
     3481 ccggtcagca tctccgcggc atcggccatc cctgcgagat cggcggcaat gttgatggtg
     3541 ttcgcgacga gcagcgcgga cgccgcggca acgagaagag gccgcgggaa cttccgacgc
     3601 agcgccccgg ccagcccatg gccggtgacc atgccgatgc gggcgcacat catctgcacg
     3661 gccgccatca gaggccacgt caggacagcc gtccagagga gtgacgtccc gaactgcgcc
     3721 ccggcgatcg aataggtcgc gattccggag gggtcgtcgt cggcggcgcc ggtgatgacg
     3781 ccgggcccga gaaccgagaa gaaacgacgg atcggtccgg gtcgtgaacg ggttctgggt
     3841 ccgctcggcg gcgtcacagc ctcatgccgc gtcacggcct cattctgcta tgggccgccc
     3901 tgcctccgtg agaagtacgt ccgggtcgcc tcgatgtccc gaccgatctg gtcggagaga
     3961 gccgtgaccg atgggaatcg ccgctcttcg cggagccgtt cgaagaagta cagtcgcacg
     4021 cgctcgccgt acacgttcgc cgaaaaatcg aggacatagg tctcgatgac ggtggcgtag
     4081 tcctcgtaga ccgtcggccg ccgtccgatg ttggtgacac agtcgaagcg ccggccgaag
     4141 gagcggatgt cgatctgggt gacgtagacc ccgtcggcgg gatgaaggtc gttctccggt
     4201 tccaggttga tcgtcgggta tccgatccga cgtccgacct tgtcgccctt ggcgacgagc
     4261 ccgtcgatcg c
//
LOCUS       DASWAV010000574         4108 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U2665
            k141_472349, whole genome shotgun sequence.
ACCESSION   DASWAV010000574 DASWAV010000000
VERSION     DASWAV010000574.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960316
            Sequence Read Archive: SRR10260007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 4108)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4108)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:47:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,929
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4108
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_472349"
                     /isolate="SMAG_U2665"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.36444 N 132.31889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..851
                     /locus_tag="VF958_11950"
     CDS             <1..851
                     /locus_tag="VF958_11950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011985632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9149866.1"
                     /translation="AEYPSATGRLTGAARDAYAFLLRFKFGAGEPLPPPDRTVSDEEY
                     AGLLFSGALRLLGITEASGKFLSREASNLWVRTPEGRVGLPVDPDLPLARRVGDRWFV
                     ASTLALRPGDRLRWWKRGSEVLGLWVELDSAGPSYERESAWTEWVRRASGRELARRMS
                     QRVAGTEVRDLTVTKRSPSGRVIEMRVVTDAAEITLRGFDVRQALEMPELLFTFSRAR
                     GPDGEIEFVFLGRGWGHGVGLCQNGAYGMALGGATYDAILKHYYTGVDIVQAAGVNAS
                     AAPSLR"
     gene            894..2042
                     /locus_tag="VF958_11955"
     CDS             894..2042
                     /locus_tag="VF958_11955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014100432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEX9149867.1"
                     /translation="MTATLTAEQQAIRQAAREFADAEIRPHVMEWDEAQHFPRDVFAK
                     LGELGFLGTIFPEKYGGAGLSYMDYIGVIEEIAAVDGSVGLSLAAHTSLGSNHIYQFG
                     TEAQRQRYIPKLATGEWLAAWGLTEAEAGSDSGGTRSTAVRDGHSWVLNGSKNFTTNS
                     SVGNVAVLMAVTDRGAGRHGISAFIVELDNPGIRVGKKENKLGMRASDTCTLVMEDCR
                     VPVENLLGSEGTGFVDSMKVLDGGRISIAALAVGMARGAWEAALKYAKERRQFGRPIA
                     DFEAIQFYLAEMATEIDAARLLTERAAQARDRGDETTRMSAQAKLYSSEIAVRVADRA
                     VQIHGGYGYIKDFPVEKFYRDVKLCTIGEGTSEIQKLVIAKSLLTEGS"
     gene            2072..3022
                     /gene="meaB"
                     /locus_tag="VF958_11960"
     CDS             2072..3022
                     /gene="meaB"
                     /locus_tag="VF958_11960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014100261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0015819 - lysine transport [Evidence IEA];
                     GO_process: GO:0015822 - ornithine transport [Evidence
                     IEA];
                     GO_process: GO:1903826 - L-arginine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl Co-A mutase-associated GTPase
                     MeaB"
                     /protein_id="HEX9149868.1"
                     /translation="MVEGVFAREPRRIAQAISAVESGEGVPDLLRAIFPRTGRARILG
                     LTGPPGAGKSTLALRIAQVYRGRGRTVGIVAVDPSSPFTGGAILGDRIRMGEIYTDPG
                     VFIRSMATRGVMGGLARATSDAVDILDAAGFDVVLIETVGVGQDEVDIVKTAETVAVV
                     LVPGLGDDIQAIKAGILEIADVFVVNKADREGADRASAELTMMLDLAPTAGDWRPPIV
                     RTVAPRGEGVEKVVEAMESHGVSLRNSPEGRRRAARRARSRLLALLAERFHRTVESRA
                     PEPDGLEDAVERVRDRSEDPYSAAGRLFERLVREPAVERA"
     gene            3019..3435
                     /gene="mce"
                     /locus_tag="VF958_11965"
     CDS             3019..3435
                     /gene="mce"
                     /locus_tag="VF958_11965"
                     /EC_number="5.1.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005659456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA epimerase"
                     /protein_id="HEX9149869.1"
                     /translation="MSVFAYRLDHIGIAVASIDESLPIYRALGLTETAREAVANQGVV
                     TAFLPLGDTRLELLEPVGADSPIAKFLAKRGPGIHHVCFAVDDLEGALADLASRGFRL
                     VNETPVPGAAGKRVAFLHPSAGNGVLIELSERASNP"
     gene            3432..3731
                     /locus_tag="VF958_11970"
     CDS             3432..3731
                     /locus_tag="VF958_11970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9149870.1"
                     /translation="MNPGQLVLVHLVGPREKFWGALVDTSPSGVTVRGLALDLFDGWM
                     REVAKGGAPSSSPATVFFPMHRVERIFADETSGEVESYAERFIGVVGRSVRDFLG"
     gene            complement(3812..>4108)
                     /locus_tag="VF958_11975"
     CDS             complement(3812..>4108)
                     /locus_tag="VF958_11975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9149871.1"
                     /translation="EREMLDWGNPLARVLHAGVASIRGHIPEATRLLQVAEEEFSRAD
                     MALYSAVAKRRRGELTGGDEGRALVGEADGWMLSEGIQNPNGMATMLAPGRWSV"
BASE COUNT          602 a         1387 c         1456 g          663 t
ORIGIN      
        1 acgccgagta cccgtcggcg accggacggc tgaccggcgc ggcgcgcgac gcctacgcgt
       61 tcctattgcg cttcaagttc ggggccggtg aaccgctgcc gcctccggat cggacggtga
      121 gcgacgagga gtacgcgggc ctcctcttct cgggcgcgct gcggcttctc ggcatcacgg
      181 aggcctccgg caagttcctg tcgcgcgagg cttcgaacct gtgggtccgc acgccggagg
      241 gccgcgtcgg ccttcccgtc gaccccgacc ttccactcgc gaggcgtgtc ggcgatcggt
      301 ggttcgtcgc ctcgacgctc gcgctgcgcc cgggcgaccg gcttcgctgg tggaaacggg
      361 ggagcgaagt gctcggcctc tgggtggagc tcgactctgc cggcccctct tacgagcgcg
      421 agtccgcgtg gacggagtgg gtgcggcgcg catccggacg cgagctcgcc cgccgcatgt
      481 cacagcgcgt cgcgggaacc gaagtgcgcg acctcacggt gacgaagcgg agtccctccg
      541 ggcgggtcat cgagatgcgg gtcgtcacgg atgccgccga gatcacgctc cgcggattcg
      601 acgtgcgtca ggcgctggag atgcccgagc tcctcttcac gttttcgcgg gcgcgcgggc
      661 ccgacggaga gatcgagttc gtgttcctcg ggcggggatg ggggcacggc gtgggtctct
      721 gccagaatgg cgcttacggc atggcgctcg gcggcgccac gtacgacgcc atcctcaagc
      781 attactacac cggagtggac atcgttcagg ccgcgggcgt caacgcgtcc gccgcgccgt
      841 ctctccgtta gccacttcgg tccagcggcc gtgggcgcgc tagaatttgc tccatgaccg
      901 cgactctgac cgccgaacag caggccatcc gccaggcggc gcgcgagttc gccgacgccg
      961 agatccgccc ccacgtcatg gagtgggacg aagcgcagca ctttccgcgc gacgtgttcg
     1021 ccaagctggg ggagctcggc ttcctcggga cgatcttccc cgagaagtac ggcggcgccg
     1081 ggctctccta catggattac atcggcgtca tcgaggagat cgcggctgtc gatgggtccg
     1141 tgggactgtc gctcgcggcc cacacctcgc tcgggtccaa ccacatctac cagttcggca
     1201 ccgaggcgca gcgccagagg tacatcccga agctcgccac cggagaatgg ttggccgcgt
     1261 ggggcctgac ggaggccgag gccggctcgg attcgggcgg cacgcgctcg acggcggtgc
     1321 gggacggcca ctcctgggtg ctgaacggct cgaagaactt caccacgaat tcgtccgtcg
     1381 gaaacgtcgc cgtgctcatg gccgtcacgg accgcggcgc gggccgccac ggcatctcgg
     1441 cgttcatcgt cgagctcgac aacccgggta tccgcgtcgg caagaaagag aacaaactcg
     1501 ggatgcgagc gtccgacacg tgcaccctcg tcatggagga ctgccgggtc ccggtcgaga
     1561 atctcctcgg ctcggaggga acagggttcg tcgactccat gaaggtcctg gatggcggcc
     1621 ggatctcgat cgccgcgctc gccgtgggga tggcgcgcgg cgcgtgggag gcggctctca
     1681 agtacgcgaa ggagcggcga cagttcggtc ggccgatcgc cgacttcgag gcgatccagt
     1741 tctacctcgc cgagatggcg accgagatcg acgcggcgcg acttctgacc gagcgcgccg
     1801 cacaggcgcg cgaccgcggc gacgagacga cccggatgtc ggcgcaggcc aaactctact
     1861 cgagcgagat cgccgtccgt gtcgccgacc gcgcggtcca gatccacggc ggttacggtt
     1921 acatcaagga ctttccggtc gagaagttct accgcgacgt gaagctctgc acgatcggcg
     1981 aggggacgag cgagatccag aagctggtga tcgcgaagag cctgctgacg gaaggatcgt
     2041 gagccggtcc ccgacgccgg ggaccgaggc gctggtcgaa ggggttttcg cgcgagagcc
     2101 ccggcgaatc gctcaggcga tctccgcggt cgagtcgggc gagggcgtcc cggacctgtt
     2161 gcgcgcgatc tttccgagaa ccggtcgtgc gcgcattctc gggctcaccg gcccgccggg
     2221 cgccggaaag tcgacgctcg ccctgcgcat cgctcaggtg tatcgcggcc gcgggcggac
     2281 cgtcggcatc gtcgcggtcg atccgtcgtc gcccttcacg ggtggcgcga tcctgggcga
     2341 ccgcatccgc atgggggaga tctacaccga cccgggcgtc ttcatccgct cgatggcaac
     2401 gcggggcgtg atgggcggcc tcgcgcgcgc cacctccgac gccgtggaca tcctcgacgc
     2461 cgcgggcttc gacgtcgtgc tcatcgagac ggtcggcgtg gggcaggacg aggtcgacat
     2521 cgtcaagacc gccgagacgg tggcggtcgt cctcgtcccg ggcctcggcg acgacatcca
     2581 ggcgatcaag gcggggattc tcgagatcgc cgacgtcttc gtcgtcaaca aggcggatcg
     2641 cgagggcgcg gaccgagcgt ccgccgagct cacgatgatg ctcgacctgg cgccgaccgc
     2701 gggcgactgg cgtcccccga tcgtccgcac cgtcgcgccg cggggggagg gggtcgagaa
     2761 ggtcgtcgag gcgatggagt cgcacggcgt ctccctgcgc aactcgccgg aaggccggcg
     2821 gagggccgcg cgacgcgcgc gttcgagact tctcgcgctt ctcgcggagc gcttccaccg
     2881 gacggttgaa tcgcgcgcgc cggagcccga cggcctcgaa gacgcagtcg agcgggtgcg
     2941 cgaccgcagc gaggatccct actcggccgc ggggcggctg ttcgagcggc tcgtccgtga
     3001 gcccgcggtg gagcgcgcat gagcgtcttc gcctatcgcc tcgatcacat cgggatcgcg
     3061 gtcgcctcga tcgacgagtc gctgccgatc taccgcgcgc tcgggctgac ggagaccgcc
     3121 cgcgaggccg tcgcgaacca gggcgtggtc accgcgttcc tgccgctcgg cgacacgcgt
     3181 ctcgagcttc tcgagccggt cggcgccgat tcgccgatcg cgaagtttct tgcgaagcgg
     3241 gggccgggga tccaccacgt gtgcttcgcg gtcgacgacc tcgagggcgc gctcgccgac
     3301 ctcgcgtcgc gcgggttccg gctcgtcaac gagaccccgg ttccgggggc cgcgggcaag
     3361 cgcgttgcgt tcctgcatcc atcggccggg aacggcgttc tgatcgagct ctcggaaaga
     3421 gcgtcgaacc cgtgaacccg ggacagctcg tcctcgtgca cctcgtcggt ccgcgcgaga
     3481 aattctgggg agccctcgtg gatacgtcac cgtcgggcgt gacggtgcgt gggctcgcgc
     3541 tcgacctctt cgacggttgg atgcgggagg tcgcgaaagg cggggcgccg tcgtcttccc
     3601 cggcgaccgt atttttcccg atgcaccgcg tcgaacgcat tttcgcggac gaaacgtccg
     3661 gggaagtcga gtcttacgcg gagaggttca tcggcgtggt cggccgaagc gttcgggatt
     3721 tcctggggta acgagtcgcc ggctcggcgc gttcgagcca tgggatgggc gtggcggccg
     3781 tttgtctttc ggcggcgtca agtcgcccgt atcaaacact ccatcgcccc ggcgcgagca
     3841 tcgttgccat cccgttaggg ttctgaattc cttcactgag catccagccg tccgcctccc
     3901 cgacgagggc gcggccttcg tcgccgccgg tgagctcgcc ccgccgcctt ttcgcaacag
     3961 ccgagtagag cgccatgtcg gcccgagaga actcctcctc ggcaacctgc agcagacgcg
     4021 tggcttccgg gatgtgtccc cggatcgaag cgactcccgc gtgtagaacc ctcgcgagag
     4081 ggtttcccca gtcgagcatc tcccgctc
//
LOCUS       DASWAV010000575         1862 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U2665
            k141_389776, whole genome shotgun sequence.
ACCESSION   DASWAV010000575 DASWAV010000000
VERSION     DASWAV010000575.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960316
            Sequence Read Archive: SRR10260007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 1862)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1862)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:47:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,929
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1862
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_389776"
                     /isolate="SMAG_U2665"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.36444 N 132.31889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..476
                     /locus_tag="VF958_11980"
     CDS             <1..476
                     /locus_tag="VF958_11980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9149872.1"
                     /translation="YVSLEGNESGTYFRGRASFQNGLARIPVPEDFRMVTDPEGLTVQ
                     ITPIGELATVAVVRMDLDEIVAKSSRNVEFSYMVNGVRKTHKHLTPIGPGHEYMPETP
                     DAKMPLYLTEGQKEMLISNGTYNRDGTVNMETARHLGWDKEWERRQRPAPQPTPE"
     gene            complement(536..>1862)
                     /locus_tag="VF958_11985"
     CDS             complement(536..>1862)
                     /locus_tag="VF958_11985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9149873.1"
                     /translation="ATRNFSLFYVPVGSSGASALRATRSVGAGQVLALNDVVFSEFGF
                     DTSLGEMTVIANDPAFIATSRAYTQSSNGTFGQFVPGFRSLGGLSFGQGFAAANGFSK
                     DSRFHTNVGFTEVSGAPVTVRIDVLDSNGSLLASTTRDGAPNQTLLLTDIIGERGLGA
                     TSNFRVNFTVTSLTGRVIPFAILIDDATGDGLFEPAGNPAPSADDVIVTQASFARGNN
                     DTFFKTNLHVTNVGGSPVTLTVSLIPRLVTGTPAAPRVYTLAPGQTIEKPDVLNTEFG
                     LQDPSAAGLRIHPSGPAQLVVSTRTFVEKFGGTFGFFIPGLPASSATVLSLGTGRVAA
                     IQLDQSSAATGSRSNFGIAEIGGADVLVRVTAKSGETGAVLGVKSYFVPANTSFQQSV
                     TDILGAGAFATNVYLQFELDGGAGRALCYGVAVDNTSGDAIYVPAQREP"
BASE COUNT          337 a          594 c          626 g          305 t
ORIGIN      
        1 gttatgtctc gctcgaaggc aacgagtccg ggacgtattt tcgcgggcgg gcgagttttc
       61 agaacggcct ggcccgaatt ccggttcccg aggatttccg catggtgacc gatccggaag
      121 gactgactgt tcagatcacg cccatcggcg agctcgccac ggtggcggtc gttcgcatgg
      181 acctcgacga gatcgtcgcg aagagctcgc gcaacgtcga gttttcgtac atggtcaacg
      241 gcgttcggaa gacccacaag cacctgaccc cgatcggacc cggacacgag tacatgcccg
      301 aaactcccga cgcgaagatg ccgctctacc tcacggaggg tcagaaggag atgctcattt
      361 cgaacggaac ctacaaccgg gacggcacgg tgaacatgga gacggcgcgg catctcggct
      421 gggacaagga gtgggagagg cgccagagac cggctccgca gccgacgcca gagtagagaa
      481 agaaaagggc cgcggtcttc cgcggccctc ggtttcccgt attccgtttc ccggctcagg
      541 gctctctctg cgccgggacg tagatcgcgt cgcccgaggt gttgtccacg gccacgccgt
      601 agcagagcgc cctccccgcg ccgccgtcca gctcgaattg caggtagacg ttcgtcgcaa
      661 aggcgccggc gcccaggatg tcggtgacgc tctgctggaa ggacgtgttc gccggcacga
      721 agtaactctt caccccgagc accgcgcccg tctcgccgct cttcgcggtc acccggacga
      781 gaacgtcggc gccgccgatc tcggcgatgc cgaagttcga gcgggagccc gtggccgcgc
      841 tcgactgatc gagctggatc gcggcgacgc gacccgtccc gagggagagc accgtcgcgg
      901 atgaagctgg cagcccggga atgaagaacc cgaaggtgcc gccgaatttc tcgacgaagg
      961 ttcgcgtcga cacgacgagc tgggcgggcc cgctcgggtg gatgcgcagg ccggccgccg
     1021 acggatcctg caggccgaat tccgtgttca ggacatcggg cttctcgatc gtctgccccg
     1081 gcgcgagcgt gtaaacccgc ggcgccgcgg gcgtcccggt gaccaggcgc gggatgagtg
     1141 agaccgtcag cgtgaccggg ctcccgccga cgttcgtcac gtgcaggttg gtcttgaaga
     1201 acgtgtcgtt gttcccgcgg gcaaaggacg cctgcgtcac gatcacgtcg tccgccgacg
     1261 gcgcagggtt ccccgccggt tcgaagaggc cgtcacccgt cgcgtcgtcg atcaagatcg
     1321 cgaaggggat gacgcgtccc gtcaggctcg tgaccgtgaa gttcacgcgg aagttcgacg
     1381 tcgcacccag gccgcgctcg ccgatgatgt cggttaggag cagagtctga ttcggtgcgc
     1441 cgtcgcgtgt cgtcgaggcg agaagacttc cgttcgagtc gagcacgtcg atccggacgg
     1501 tcaccggcgc gcctgaaacc tcggtgaacc cgacgttcgt atggaacctc gagtccttcg
     1561 agaagccgtt cgccgcggcg aatccctgac cgaaggacaa tccgccgagg ctgcggaatc
     1621 ccggcacgaa ttgtccgaag gtcccgttcg agctctgcgt gtaagcgcgg ctcgtcgcga
     1681 tgaaggcagg atcgttggcg atgaccgtca tctcgccgag cgaggtgtcg aaaccgaact
     1741 ccgagaagac gacgtcgttc agcgcgagaa cctgtcccgc gccgacgctg cgcgtcgcac
     1801 ggagcgcgct cgctccgctg gatccgaccg gaacgtagaa gagcgagaag tttctcgtgg
     1861 cg
//
LOCUS       DASWAV010000576         8223 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U2665
            k141_71407, whole genome shotgun sequence.
ACCESSION   DASWAV010000576 DASWAV010000000
VERSION     DASWAV010000576.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960316
            Sequence Read Archive: SRR10260007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 8223)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8223)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:47:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,929
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8223
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_71407"
                     /isolate="SMAG_U2665"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.36444 N 132.31889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..712)
                     /locus_tag="VF958_11990"
     CDS             complement(<1..712)
                     /locus_tag="VF958_11990"
                     /inference="COORDINATES: protein motif:HMM:NF013188.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HEX9149874.1"
                     /translation="MPDLLALLALAAAVLVLAGLTEGFVTRAPLSFPMVFLGLGFLLG
                     HRGVIAIAPHSGILEAVGVVTLSLILFLDAVRMKFDRSAKEWLVPALVLGPGTILCLL
                     LVATAAHVLFGTGIAASILLGAILSSTDPVVVRDIVRDTRVPGAVRQTLKVEAATNDV
                     VVLPVVLVTIAISKASAATFGQWALLLGKLIVIGPAIGFTVGALGAWLVTQVDRRFEI
                     RREYQALFGMGLVFTSYTA"
     gene            806..4045
                     /locus_tag="VF958_11995"
     CDS             806..4045
                     /locus_tag="VF958_11995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9149875.1"
                     /translation="MKSSRNVLAGVLFLSLGGAVFAAEAPKKPAAAEKKTAAPPAAQE
                     ETAGPQRGFKFRNLGPAAGGGRITAIAGIPGNPNVIYVGSASGGVWKTVDGGVSFKPM
                     FDEYPPSIGAVAVAPSNPNLVWVGTGEANPRNNVIDGHGVYFSPDAGASWRFMGLADA
                     GQISRILIDPGDPNTVYVAALGGVWKPNAERGIFRTTDGGETWKKVLFVDGETGASDL
                     AMQPGNSRVLLAGMWQFRRFPWELVGGGPGSGIWKSTDGGATWRKIEKDMPEGPLGRV
                     SIAFSPSNGNHVYALIHAKKGILFESKDLGEKWEKLADTRLINVRPWYFSVIAVSPAD
                     ENRLFFGSFNLVTSIDGGKTFETNNRRIHPDYHALWIDPKDPDRILQGNDGFALASTD
                     GGKTWRSFDLPLGQFYQVAVGSDAPYTICGGLQDNNGWCGASNSLARGGVSDADWFVV
                     TGGDGEWVVPAPSDPNVIYADSQNGNISRFDRRTKLSRNIRPYLSGAGDMAPADLKYR
                     FNWTSPIAVSANDVNEVYVGGNVVFKSTDGGLHWAVVSPDLTRNDKSKQKASGGPVHL
                     DLSGAETYDTLLSLAISTVDPKVIWAGSDDGLVHVTKDGGETWSNVSPRGAPEWARVY
                     QIDPSPFDAGTAHLLYDAHMLGDRRPHVYGTTDFGKSWTDLSKGLPADGTAYVVRENP
                     NKKGFLVVGTDDGLHSSNDGGRTWEKLPVEFPTVPVWDVKFVPASHDLVVASHGRGMW
                     IFDDITPLEETGPAIEDKDLHLFTVPPAKLWQIWNRGGFGVGDWVAPNPPPGAIIDYF
                     LKNEIKPTEEQKKQKREPVKITVRDSKGSLVATGYGPGRQGINRYVWQLRYEGPKKIG
                     FGREAPPSEFFDPNRGPDVVPGVYKVTVDAAGKSETGDLQVGPDPRWPADPEALRARA
                     KAALEGRNAASAINEMLNRLDAWETQLTGLPKLVGGGDDGDPARATKYETALSAARDL
                     NKKVKELKDSVYNRDVQRDTPSDSLHFHNDFQGRVSRLGFLAGAYGEAPREVAREELA
                     VIRKQAEDYLARFNALVTGDVPAYNRAAAQQGVPTLFAGDPIRVEEPAL"
     gene            complement(4076..4798)
                     /locus_tag="VF958_12000"
     CDS             complement(4076..4798)
                     /locus_tag="VF958_12000"
                     /inference="COORDINATES: protein motif:HMM:NF012579.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HEX9149876.1"
                     /translation="MVDTRNKKPAPDPKAMMTVAEVSSYLSLSAGTVYKLVGDLRAVR
                     IGGRWCFRTADVEQWLLKRRSAGEPPIEPVQELGSHVRLIPHLDESNIFLDVSDSDAP
                     TLIANAIRRARLDLTESPKEAAKERIIASILEREALCSTALHPDVAFPHPRDPEKCPL
                     GRDHIVVVRAAKPVEFGEIHGHRPRIVFVLLARTVSLQLHWEARLSHLLHREDFVEKA
                     LTAKSAREFRELFGPAPRTSDA"
     gene            4944..5666
                     /locus_tag="VF958_12005"
     CDS             4944..5666
                     /locus_tag="VF958_12005"
                     /inference="COORDINATES: protein motif:HMM:NF012579.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HEX9149877.1"
                     /translation="MEDRLMTIKKLADYLSVNDRTVLKLVSDGTLPGVKVGNQWRFRK
                     AMIDTWLDDQMLGVTPRYLDAPGLDGAPRRMLELTSCFQPDHVIPELTARTRNTVIEE
                     LAGLANRLGLVRDKTWFVGALIERENVMPSGMGNGIAFLHTLRRNPEQITRPFMVLGR
                     SHEGVDFDALDGKPTHLFFVLGLKFDELFLPWLHKLSQMFLRDETVRAVLDAPDAEAI
                     FDAVAQAERSLEPEPRVGSERR"
     gene            5670..6203
                     /locus_tag="VF958_12010"
     CDS             5670..6203
                     /locus_tag="VF958_12010"
                     /inference="COORDINATES: protein motif:HMM:NF014630.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-type zinc ribbon domain-containing protein"
                     /protein_id="HEX9149878.1"
                     /translation="MEATIRDLTDLSGIDEQLGEKTAPDAAVLASLEKRRARLRKKIP
                     GLLLASYDALSRAGRRPVVVPLQNAHCSGCRLRLPPQLDSSIRRHRTLCPCPYCHRLL
                     YAPAADAHAKQGDGPAHSHRVPKVKGAGSISVRGTPPPSKPEPRGKKTRERNPRDLRP
                     KSRTHRLSERAGADHVN"
     gene            6288..7703
                     /locus_tag="VF958_12015"
     CDS             6288..7703
                     /locus_tag="VF958_12015"
                     /EC_number="1.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016196272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Glu/Leu/Phe/Val dehydrogenase"
                     /protein_id="HEX9149879.1"
                     /translation="MSTSTSLLDTVNRNLDAAAAHGDYPHGLLEQIKTCNSLYRFYFP
                     VRREGGGYEVIEAWRAEHSHHKLPVKGGIRYSPEVDADEVQALAALMTYKCAILDIPF
                     GGAKGAIRIDSRRRSTEELERITRRYAAELIRKNFIGPGIDVPAPDYGSGAREMAWIA
                     DTYVQFHPGEIDAIGCVTGKPVSQGGIRGRSEATGRGVFYGVREALDDTELTRKHGLA
                     PGLAGKRVVVQGLGNVGYNAARFLAEGGAVIVGLAEYEGAISNPGGLDLESVVRHRKE
                     TGRITGFPGAKDLPSNAAALELDCEVLVPAALENVLTVENAGRVKARCIAEAANGPTT
                     TDAEAILRSRGILVIPDMYLNAGGVTVSYFEWLKNLSHVRFGRMAKRFEELSAHRMVT
                     AIESATGRELAKGERSRLIRGPDEEDIVNSGLEEAMATAFRQIRDMQAAKPKIPDLRT
                     AALAVAIDKIALSYMELGIFP"
     gene            7794..>8223
                     /locus_tag="VF958_12020"
     CDS             7794..>8223
                     /locus_tag="VF958_12020"
                     /inference="COORDINATES: protein motif:HMM:NF012260.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta/gamma crystallin-related protein"
                     /protein_id="HEX9149880.1"
                     /translation="MKGRSLRSITLPMLLAVFGTGLAVAQQPYRDYRPGAGGITVYQD
                     ENYRGKTATFREDVPDLRQYGLNDRITSLRIAPGESWEVCRDTNYRGQCQVFTGDDRD
                     LRRSGWTDKISSLRRVRGGGPGRFPAPPITKEGIILYDQPY"
BASE COUNT         1549 a         2697 c         2753 g         1224 t
ORIGIN      
        1 ccgcggtgta ggaggtgaag acgagtccca ttccgaagag ggcctgatac tcgcgccgga
       61 tctcgaaacg ccggtccacc tgcgtcacga gccacgctcc cagcgcgccc acggtgaagc
      121 cgatcgccgg tccgatgacg atgagcttgc cgagaaggag agcccactgc ccgaatgtcg
      181 cggcgctcgc cttcgagatc gcgatcgtaa cgagcacgac cggcagtacg acgacgtcgt
      241 tcgtggccgc ctcgaccttc agagtctggc ggacggctcc cgggactctc gtgtcgcgga
      301 cgatgtcgcg cacgaccacg ggatcggtcg aggagaggat tgccccgagc aggatggacg
      361 cggcgatgcc cgtcccgaag agaacatggg cggcggtcgc gaccaggagg aggcagagaa
      421 tggtcccggg gccgagcacc agagcgggaa cgagccactc ctttgcggag cgatcgaact
      481 tcatgcggac ggcgtcgaga aacaggatga gagacagcgt cacgacgccg acggcctcga
      541 ggatcccgga gtgtggcgcg atcgcgatca ccccgcggtg gcccagcagg aatccgaggc
      601 cgaggaagac catcgggaac gagagaggcg cccgtgtgac gaacccttcc gtcaggcccg
      661 cgagcacgag cacggcagcg gcgagcgcga gaagggcgag aagatcgggc acggggagat
      721 tctacggttc ggccggcggc gccgacagtt gtcggtcggt tcgtgcgcgc gaaataatcg
      781 gcgcttcgca agagtgagga gagagatgaa gagctcacga aacgttctgg cgggcgtcct
      841 gttcctctcg ctcggagggg cagtctttgc ggcggaagcc ccgaagaagc cggctgcggc
      901 ggaaaagaag accgccgcgc cccccgcggc gcaggaggaa acggccgggc cgcagcgcgg
      961 cttcaagttt cgcaacctcg gtccggccgc gggcggcgga cgcatcaccg cgatcgcggg
     1021 catcccgggc aacccgaacg tgatctacgt cggctcggcg tcgggcggag tgtggaagac
     1081 ggtcgacggc ggcgtgtcgt tcaagccgat gttcgacgag tacccgccct cgatcggagc
     1141 cgtcgcggtc gctccgtcca acccgaacct cgtgtgggtg ggaacgggcg aagcgaaccc
     1201 gcgcaacaac gtcatcgacg ggcacggcgt ctacttctcc cccgacgccg gggcgagctg
     1261 gcgcttcatg ggcctcgccg acgccggcca gatctcgcgc atcctgatcg acccgggcga
     1321 cccaaatacc gtctacgtcg cggcgctcgg cggcgtctgg aaaccgaacg cggaacgcgg
     1381 gatcttccgc acgaccgacg gcggcgagac gtggaagaag gtcctcttcg tggacggaga
     1441 gaccggcgcg tccgacctcg cgatgcagcc gggcaactcc cgggtgctgc tcgccggcat
     1501 gtggcagttc cggcgattcc cgtgggagtt ggtcggcggc ggcccgggaa gcgggatctg
     1561 gaagtccacc gacggcggag cgacgtggag gaagatcgag aaggacatgc cggagggccc
     1621 gctcgggcgc gtttcgatcg cgttctctcc ctccaacggc aatcacgtct acgcgctgat
     1681 ccacgcgaag aagggaatcc tcttcgagtc gaaggatctc ggcgagaagt gggagaagct
     1741 cgcggacacg cggctcatca acgtgcggcc ctggtacttc tcggtgatcg cggtctcgcc
     1801 ggccgacgag aacaggctct tcttcggctc cttcaacctc gtcacgtcga tcgacggcgg
     1861 caagacgttc gagaccaaca accggcggat ccatccggac taccacgccc tctggatcga
     1921 tccgaaggat ccggaccgga tcctccaggg caacgacggc ttcgcgctcg cctccaccga
     1981 cgggggcaag acctggcgga gcttcgacct gcccctcggc cagttctacc aggtggcggt
     2041 gggttccgac gccccctaca cgatctgcgg aggcctccag gacaacaacg gctggtgcgg
     2101 cgcttccaac tcactcgcgc gcggcggcgt ctccgacgcc gactggttcg tcgtgacggg
     2161 aggcgacggc gaatgggtcg tgcccgcccc gtcggatccc aacgtgatct acgccgactc
     2221 gcagaacgga aacatctcgc gtttcgaccg ccggacgaag ctctcgcgca acatccggcc
     2281 gtatctgtcg ggcgcgggag acatggcgcc ggcggacctg aagtaccgct tcaactggac
     2341 ctctccgatc gcggtctcgg caaacgacgt caacgaggtg tacgtcggcg ggaacgtcgt
     2401 cttcaagtcg accgacggcg gtctccactg ggccgtcgtc agcccggacc tgacccgaaa
     2461 cgacaagtcg aaacagaagg cctccggcgg tccggtccac ctcgacctct cgggcgccga
     2521 gacgtacgac acgctgctct cgctcgcgat ctcgaccgtg gatccgaaag tcatctgggc
     2581 gggctccgac gacggcctcg ttcacgtcac gaaagacggc ggcgagacgt ggagcaacgt
     2641 ctcaccacgc ggtgcgcccg agtgggcacg cgtctatcag atcgacccct cccctttcga
     2701 cgcggggacg gcccacctgc tgtacgacgc gcacatgctc ggcgaccgcc gccctcacgt
     2761 ctacggcacg accgatttcg ggaagtcgtg gacggacctg tcgaagggac tcccggccga
     2821 cggcacggcc tacgtcgtgc gggagaaccc gaacaagaaa ggtttcctcg tcgtcggcac
     2881 cgatgacggc ctgcactctt cgaacgacgg cggccgtacc tgggagaagt tgccggtcga
     2941 attcccgacc gtgcccgtat gggacgtcaa gttcgtcccg gcttcgcacg acctggtcgt
     3001 cgcctcgcac ggccgcggca tgtggatctt cgacgacatc acgccgctcg aggaaacggg
     3061 cccggcgatc gaagacaaag acctccatct cttcaccgtt ccgccggcga agctctggca
     3121 gatctggaat cgtgggggct tcggcgtcgg cgattgggtc gcgcccaatc cccctccggg
     3181 agcgatcatc gactacttcc tgaagaacga gatcaagccg accgaggagc aaaagaagca
     3241 gaagcgcgag cccgtgaaga tcacggtccg cgattcgaag gggagtctcg tcgcgaccgg
     3301 atacgggccg ggcaggcagg gaatcaaccg gtacgtctgg cagctccggt acgaaggacc
     3361 gaagaaaatc gggttcggcc gagaggcgcc gccctccgag ttcttcgacc cgaaccgcgg
     3421 gccggacgtg gtccccggcg tctacaaggt cacggtcgac gcggccggaa agagcgaaac
     3481 cggagatctt caggtcgggc ccgatccgcg ctggccggcc gaccccgaag cgctccgcgc
     3541 tcgggcgaag gcggccctcg aggggcgcaa cgcggcatcg gcgatcaacg agatgttgaa
     3601 ccgtctcgac gcatgggaga cgcagctgac ggggcttccg aagctcgtcg gaggcggcga
     3661 cgacggcgat cccgcgcggg cgacgaagta cgagacggcc ttgtcggccg cccgcgacct
     3721 gaacaagaag gtcaaggaac tgaaggacag cgtgtacaac cgcgacgtcc agcgcgacac
     3781 gccgtcggat tcgctgcact tccacaacga cttccagggc agggtttcac gtctgggctt
     3841 cctggccggc gcttacggcg aagcgccccg ggaggtggcc cgggaggagc tggcggtgat
     3901 ccgcaagcag gccgaggact acctcgcccg cttcaacgcg ctcgtcaccg gcgacgtccc
     3961 cgcttacaac cgggcagccg cgcagcaggg ggtccccacg ctcttcgcgg gcgacccgat
     4021 ccgggtggaa gaaccggcgc tttgacgaac cccgaaactt ctgccgcgat cgagatcagg
     4081 cgtcggaggt tcgaggagcg ggcccgaaca actcgcggaa ctcgcgggcg gatttcgccg
     4141 tgagtgcttt ctcgacgaag tcctcccgat ggaggagatg actcaaccgg gcttcccagt
     4201 ggagctgcag ggagaccgtt cgcgccagaa gcacgaagac gattcgcggc cggtggccgt
     4261 ggatctctcc gaactcgacg ggcttcgcgg cccggacgac gacgatgtgg tcgcggccga
     4321 gcgggcattt ctcggggtcg cgcggatgcg ggaaagcgac gtcgggatgg agcgccgtgc
     4381 tgcagagcgc ttcccgctcc aggatcgagg cgatgatccg ctctttcgcc gcctccttcg
     4441 ggctctccgt cagatcgagc cgggcccgac gaatcgcgtt ggcgatcagc gtcggtgcat
     4501 cagagtcgga gacgtcgaga aagatgttgg attcgtcgag atgcgggatg agccggacgt
     4561 gggaaccgag ctcctgcacg ggctcgatcg gaggctcgcc ggctgaccgc cgcttcaaga
     4621 gccactgctc cacgtcggct gtccggaagc accagcggcc gccgatgcga acggcccgaa
     4681 gatcaccgac gagcttgtag accgtgccgg cggagagcga gagatagctc gacacctccg
     4741 cgaccgtcat catcgctttc ggatcgggcg cgggcttctt gttccgggtg tccaccatcg
     4801 gttgacctcc agcaggactg acggagcgtc gaaggcttgc ggagcctttc gacgtattcc
     4861 gaggtagtat tgaggaattc tttgacaaca aggacactct aaggtaaaag aacgtgtatt
     4921 gtcaacgcga aaggaggtaa cggatggaag accggctcat gacgatcaaa aagctcgccg
     4981 attacctcag cgtcaatgac cgcacggtcc tgaagctcgt ttccgacggg accctgcccg
     5041 gcgtcaaggt cgggaaccag tggcgctttc gaaaagcgat gatcgacacc tggctggacg
     5101 accagatgct gggggtcacg ccacgttacc tcgatgcccc ggggctcgac ggagcccccc
     5161 gtcggatgct cgagctcacg agttgcttcc agccggacca cgtcatcccc gagctcaccg
     5221 cccggacgcg caacacggtc atcgaagagc tcgcgggtct cgccaaccgg ctcgggctcg
     5281 ttcgcgacaa gacctggttc gtcggagcgc tcatcgagag agagaacgtc atgccgagcg
     5341 gcatgggaaa cggcattgcg tttctccaca cgctccgccg caatcccgag cagatcacgc
     5401 gaccgttcat ggtgctcggc cggtcgcacg aaggtgtgga cttcgacgcc ctcgacggca
     5461 aaccgaccca cttgtttttt gtcctcggcc tcaagttcga cgagctcttc cttccctggc
     5521 ttcacaagct gtcgcagatg ttcctgcgcg atgaaaccgt tcgggccgtc ctggacgcgc
     5581 ccgacgccga ggccatcttc gacgcagtgg cgcaggccga gcgcagcctg gagccggagc
     5641 ccagggtcgg ctcggagcgg cggtaagccg tggaagcgac cattcgcgat ctgaccgacc
     5701 tctccgggat cgacgaacag ctcggtgaaa agacggcgcc cgacgccgca gtccttgcct
     5761 cgctcgagaa gcgccgggcc cggcttcgaa agaagatccc cgggctcctc ctcgcgagtt
     5821 acgacgccct ttcccgcgcc ggccggcgcc cggtcgtcgt ccctctgcag aacgcgcact
     5881 gcagcggctg tcgcctgcgc ctcccgccgc agctcgactc ctcgatccgc cgtcaccgaa
     5941 ccctctgccc ctgcccctac tgtcatcgcc tcctctacgc gcccgcagcc gacgcccacg
     6001 caaaacaggg cgacggcccc gctcactcgc atcgggtccc gaaggtcaag ggagccggat
     6061 cgatcagcgt tcgaggaacg ccgcctccgt cgaaaccgga accgcgcggc aagaagaccc
     6121 gggaacgaaa ccccagagac ctccgcccca agtcccgaac gcatcgactg tcggaacgcg
     6181 ccggcgccga tcacgtaaac tgacgagcgt cgaacccggg aacgggttct ccttcccgcc
     6241 ggacggccgg cggaaaaacc ggacgactgc gacaggagcg aaacgagatg tcgacttcga
     6301 ccagccttct ggacaccgtc aaccggaatc tcgatgccgc cgcggcccac ggggactacc
     6361 cgcacgggct tctcgagcag atcaagacct gcaacagcct ctatcgcttc tactttccgg
     6421 tgcgaaggga ggggggcggt tacgaggtca tcgaggcctg gcgcgccgag cacagtcatc
     6481 acaagctccc ggtcaagggg ggcattcgct actcgcccga agtggatgcc gacgaggttc
     6541 aggctctcgc ggccttgatg acctacaagt gcgcgatcct ggacatcccg ttcggagggg
     6601 ccaagggggc gatccggatc gactcgcgca ggaggagcac ggaagagctc gagcggatca
     6661 cgcggcgata cgcggcggag ctgatccgca agaacttcat cggccccggc atcgacgtgc
     6721 cggcgcccga ctacggcagc ggcgcgcggg agatggcgtg gatcgcggac acctacgtgc
     6781 agtttcatcc gggcgagatc gacgcaatcg gctgcgtcac cggcaagccg gtcagccagg
     6841 gcggtatccg cggccgcagc gaagcgaccg ggcggggagt cttctacgga gtccgcgaag
     6901 cgctcgacga cacggagctc acccgcaaac acggcctcgc gccggggctg gccggaaagc
     6961 gcgtcgtcgt ccaggggctc ggcaacgttg gctacaacgc cgcccggttc ctcgccgagg
     7021 gaggcgcggt gatcgtgggc ctggcggagt acgagggagc catttccaac cccgggggac
     7081 tcgacctcga atcggtcgtg cgccaccgca aggagaccgg ccggattacg ggattccccg
     7141 gagcgaagga tcttccttcg aacgcggccg cgctcgagct cgactgcgaa gtcctcgtgc
     7201 cggcggcgct cgaaaacgtc ctcacggtcg aaaatgccgg gagggtgaag gcgcgctgca
     7261 tcgcggaggc agcgaacggc ccgacgacca cggacgcgga agcgatcctg cgttcccggg
     7321 gcatcctcgt catccccgac atgtacctga acgccggcgg cgttacggtt tcctacttcg
     7381 agtggctgaa gaacctctcg cacgtccggt tcggccggat ggcgaagcgg ttcgaggagc
     7441 tctctgcgca caggatggtg accgcgatcg agagcgcgac cgggagggaa ctcgcgaaag
     7501 gcgagcgcag tcgcttgatc cgcgggccgg atgaggagga catcgtcaac tccgggctcg
     7561 aggaagcaat ggcgaccgcg ttccggcaga tccgggacat gcaggccgcc aaaccgaaga
     7621 tcccggatct ccgcacggcg gcgctcgccg tggcgatcga caagatcgcg ttgagctaca
     7681 tggagctggg aatctttccg tagcgagtcg cctgacgcgc gtcgcttgac aacccggcag
     7741 ggcggcgaaa ggatcggacg gcgatcgcgg ccgaccgcga aaaggagttc gacatgaagg
     7801 gacgatcttt gcgaagcatc acgctgccga tgctcctggc ggtcttcggc accggcctgg
     7861 cggtggcgca gcagccgtac cgggactatc ggcccggcgc cggagggatc accgtttacc
     7921 aggatgaaaa ctaccgcggg aaaacggcca cgtttcggga ggacgtcccg gatctgcggc
     7981 aatacggtct gaacgaccgg atcacgagtc tgcggatcgc gcccggagag tcctgggaag
     8041 tatgtcgaga caccaactac cggggacagt gccaggtctt caccggcgac gaccgcgacc
     8101 ttcggcgctc cggctggacc gacaagatct cttctctgcg tcgcgtgcgg ggcggcggtc
     8161 cggggagatt ccccgctccc ccgatcacga aggaaggcat catcctctat gaccagccct
     8221 att
//
LOCUS       DASWAV010000577         1694 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U2665
            k141_233164, whole genome shotgun sequence.
ACCESSION   DASWAV010000577 DASWAV010000000
VERSION     DASWAV010000577.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960316
            Sequence Read Archive: SRR10260007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 1694)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1694)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:47:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,929
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1694
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_233164"
                     /isolate="SMAG_U2665"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.36444 N 132.31889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..248)
                     /locus_tag="VF958_12025"
     CDS             complement(<1..248)
                     /locus_tag="VF958_12025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="An RNA-DNA helicase that actively releases nascent
                     mRNAs from paused transcription complexes; Derived by
                     automated computational analysis using gene prediction
                     method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HEX9149881.1"
                     /translation="MLYIPPKENQPGVLVASSTNYLPTPKDPIVPRDLIAREGLEAGA
                     LIGGFAADGGRPVVKRIDTVEGLTPAQFRQRPAFQELVS"
     gene            complement(595..>1694)
                     /locus_tag="VF958_12030"
     CDS             complement(595..>1694)
                     /locus_tag="VF958_12030"
                     /inference="COORDINATES: protein motif:HMM:NF012472.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEX9149882.1"
                     /translation="VTPGRATLDGTKRFADRAKAAAGHFRDAAGVRLSSIGLGTYLGD
                     EDAATDEGYEESIAVALASGANVLDSAINYRGQKSERAIGRALARAFASGAAHRDEVF
                     VSTKGGYLPHDSDDPRDPRRYILETFVSTGIAPREEIAAGGNCIAPGYLADQIDRSRR
                     NLGLETIDLYYLHNIESQKTSLDATAFHARLVKAIEALEKAAGEGKIARWGLATWDGL
                     RVPPEHPEHLSLADTVAAAEEVAGADHHFTAVQLPNNLAMAQAVVFRSQPSGEGRVPA
                     LTAATALGLAAFGSASLLQGRLAGELPDEIEAAFPKVPPGARRALQFSRSSPGMTTAL
                     VGVSTPEHAREDFALAGIPPADSVRVLALFA"
BASE COUNT          253 a          568 c          612 g          261 t
ORIGIN      
        1 gagacgagct cctggaaagc cggccgctgg cggaactggg cgggggtcaa gccctcgacg
       61 gtgtcgatgc gcttgaccac cgggcggccg ccgtcggcgg caaacccacc gatgagcgca
      121 cccgcctcca agccttcgcg cgcgatcagg tcgcggggga cgatggggtc cttgggagtc
      181 gggaggtaat tcgtgctcga ggcgacgagg acgcccggct ggttttcctt cggagggatg
      241 tagagcacgc cttcgaccgg tcgttcgggt ccggtcgccg cgagctcctc ggcacgctcg
      301 ggcgccccat tcgggccgaa cccggaacgg ccgccgcccc gccggcgacg gcgccggcgg
      361 cgccggtgcg ggcgcggctg cccctgtccc tctccgggga gaggctcgcg cgcttgatgc
      421 ctgaggctcg gagaggtcgc ttcttccgcc gaaacggcaa aggacgtagc gcttttcggg
      481 gtcgagggat cttcggacat gagctcctgg attccgccgc gctgactcgc tgtccgggcg
      541 ggagaatccg attctagcct aaaaccgtcc cggggcgaaa cccgatcttt cgactcaagc
      601 gaagagcgcg agaacccgga cagagtctgc cgggggtatc cccgccaacg cgaagtcctc
      661 gcgggcgtgc tcgggcgtcg aaacgccgac gagggcggtc gtcattccgg gggaagagcg
      721 ggaaaactgg agcgcccgtc gggcgccggg agggaccttc ggaaaggccg cctcgatctc
      781 gtccggcagc tcgccggcga ggcggccctg aaggagcgac gcggacccga acgccgcgag
      841 cccgagcgcc gtggccgcgg tgagggccgg gacgcggccc tcgccggaag gctgcgaacg
      901 gaagacgacc gcctgcgcca tcgcgaggtt gttcggaagc tgcaccgcgg tgaagtgatg
      961 gtccgcgcca gcgacctcct cggcggcggc caccgtgtcg gcgagcgaaa ggtgctcggg
     1021 atgctcgggc ggcacccgca ggccgtccca cgtcgcgagt ccccaccgcg cgatcttccc
     1081 ctccccggcc gccttctcga gggcttcgat cgctttcacg agacgtgcgt gaaaggccgt
     1141 cgcatcgagc gaggtcttct gtgactcgat gttgtggagg tagtaaaggt cgatcgtctc
     1201 gagaccgaga ttgcgtcgac tgcggtcgat ctggtcggcg aggtatccgg gcgcgatgca
     1261 attgcctccg gccgcgatct cctcgcgcgg cgcaatcccg gtcgagacga acgtctcgag
     1321 gatgtaccgg cgcgggtccc gcggatcgtc ggagtcgtgg ggcaggtaac cgcccttcgt
     1381 cgaaacgaag acctcgtcgc ggtgggcggc gccgctcgcg aacgcacggg cgagagcccg
     1441 tccgatcgcg cgctcgctct tctgcccgcg atagttgatc gccgagtcga ggacgtttgc
     1501 acccgatgcg agcgcgaccg cgatcgattc ctcgtaccct tcgtcggtcg ccgcgtcctc
     1561 gtctccgaga tacgttccga gtccgatcga ggagagacgg acgccggcgg cgtcccggaa
     1621 atgtcctgcc gctgccttcg cgcgatccgc gaaccgcttc gtgccgtcga gagtggcgcg
     1681 cccgggagtc accc
//
LOCUS       DASWAV010000578         4851 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U2665
            k141_588883, whole genome shotgun sequence.
ACCESSION   DASWAV010000578 DASWAV010000000
VERSION     DASWAV010000578.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960316
            Sequence Read Archive: SRR10260007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 4851)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4851)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:47:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,929
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4851
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_588883"
                     /isolate="SMAG_U2665"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.36444 N 132.31889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1088)
                     /locus_tag="VF958_12035"
     CDS             complement(<1..1088)
                     /locus_tag="VF958_12035"
                     /inference="COORDINATES: protein motif:HMM:NF012722.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase dimerization/phospho-acceptor
                     domain-containing protein"
                     /protein_id="HEX9149883.1"
                     /translation="MSLLRPRRARTRIALAGAALSAVLLLAVALGARTTIRELTFRDI
                     DEELRTLAIAVGSEFEVEGMEPQEALGKGLAANVFEFRLQNHSAILFQGDRMLALSGD
                     LLRSLGSLSLDPYRAHSEEPYTAVEPYSGQRRLCRFLVLHLGGKARGGTLVLFRSIDS
                     AMRSLRRLDRVLAALVLAGFLGTAAILAFVLDRALRPVEEVTSLARSVQASDLSRRVR
                     VSTGGEEFHELTGVINSLLERLEHAFAAQQRLVADAAHELKTPIAVLLGEAQDALRAE
                     SLPEERQKSLETMEATARGLAREVENLLSLARADAAMTAAFGVVDLATVAAQSVESSR
                     SLAAARGVRLELLPENGAPVRGDRSALF"
     gene            complement(1085..1750)
                     /locus_tag="VF958_12040"
     CDS             complement(1085..1750)
                     /locus_tag="VF958_12040"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX9149884.1"
                     /translation="MRALVVEDDLRLRSLVVRTLTRAGLACDEASRISEAEELLSLHE
                     YDLLVLDRRLPDGDGLEVCRRARRAGFGRSVLMLTALDDPASTVEGLSDGADDYLGKP
                     FDLDVLAARAHALLRRNEARAPVELVSGDLRLDPARRIALLRDETLVLTAREFAILET
                     LMRNPGSAMSRETILEHAWGEREEPMSNTIDVLIARLRKKLEAGGSSSRIETVRGVGY
                     RLT"
     gene            1803..3410
                     /locus_tag="VF958_12045"
     CDS             1803..3410
                     /locus_tag="VF958_12045"
                     /inference="COORDINATES: protein motif:HMM:NF018899.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="POTRA domain-containing protein"
                     /protein_id="HEX9149885.1"
                     /translation="MSRRGKAGSLLAIAVSLFAGVAGGAPELRAQEGPRIRRIVIETE
                     NVFQDAESSFLARAADAIHGITHEHVVRRELLFQEGDTLDPKKLLETERNLRALGFFR
                     RVEIKATPVGDDEVDVLVRTRDAWTTELSGSVGRAGGQGHYGVSLEEKNLFGLGKKVS
                     FAAAQRPDRSTREVLYSDPQFLGRRLALDVFYGSNSDGDRRHFSLESGFRSLDSPSGA
                     VLSFDQGSRQTRLYSEGSELARFGMDTRSVELSGGRRLTPAGDRPVLRLFAGYRREEA
                     SFGSIEHAPDGFALPADRKFGFFFARLELAHPEYSVQHDVAFFLRDEDFDLGNTASLE
                     LGYSPNVFGAEQAVGGALVLARGLRLPGGFLVGSLSAATRARGGSFENTLAEGQLLAV
                     WRPAETSANTLVGRFLLSVGNRLDREVQLEADGANGLRAYRLHAFAGDRRVILNLENR
                     VRLTPELLHLIVLGAAVFVDAGYAWPPGVPVRLSDLRADAGVGLRVGLPRASRHALFR
                     LDLAYALRPDIQGRRGWLVSFSSSQAF"
     gene            3484..>4851
                     /locus_tag="VF958_12050"
     CDS             3484..>4851
                     /locus_tag="VF958_12050"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEX9149886.1"
                     /translation="MTLPTGSRLGPYEILAPLGAGGMGEVYRARDSRLGRDVALKVLP
                     QELSEDRDRLSRFEQEARSASALNHPNIVTVHDIGRSDSTSYIAMELVDGRTLRELAA
                     PGPMPTRRILGIAAQIAEGLAKAHAAGIVHRDLKPENVMVSKDGFVKILDFGLAKLVE
                     PESGGLSVMPTLAKPETHPGVVMGTVAYMSPEQASGEPVDFRSDQFSLGSILYEITSG
                     QRPFQKKTAAETMSAIIREEADPIGKVRPEVPPPLRWIVERCLAKDPEERYASTRDLA
                     RELASVRDHVSEITGAAEPPIDLGPRPLKRVRRSTVLATVFLLLLFGVVGGWLVASKR
                     LPKTSAPTFHRLTFRSGSLQNARFTPDGQTIVYGATWAGEKSGLYAVRPESPESRPFE
                     IPADILAVSPAGEMAVLLNQNPISGTLARVPFAGGVPRPIVEGVPYASADWTPDGKDL
                     VIVRYV"
BASE COUNT          775 a         1642 c         1636 g          798 t
ORIGIN      
        1 tgaaagagcg ccgatcgatc gcctcggacg ggagcgccgt tttcgggtag gagctccagc
       61 cgcacgccgc gagcggctgc aagggaccgc gaagattcga ccgactgcgc ggcgaccgtg
      121 gccaggtcca cgaccccgaa agcagccgtc atcgccgcgt ccgcgcgggc gagcgagagc
      181 aggttctcga cctcacgggc gagcccccgc gcggtcgcct ccatggtctc gagagacttc
      241 tggcgctcct ctggcaggct ctccgcccgc agcgcgtcct gcgcctcccc gaggaggacc
      301 gcgatcggcg tcttcagctc gtgagcggcg tcggcgacga gccgttgctg tgcggcgaat
      361 gcgtgctcga ggcgttcgag cagagagttg atgacgccgg tcagctcatg gaactcctcg
      421 ccgcccgtcg acacgcggac gcgccgcgaa agatcggaag cctgaaccga tcgcgcgagg
      481 ctcgtgacct cctctaccgg acgcagcgcg cggtcgagca cgaacgcgag aatcgcggcg
      541 gttcccagaa aaccagccag cacgagcgcc gcgagcaccc ggtcgagccg cctgagcgac
      601 cgcatcgccg agtcgatcga gcgaaagagc acgagtgtgc cccctctcgc cttgccgccc
      661 aggtgcagaa ccaggaagcg gcagagacgc ctctgtccgc tgtacggctc gacggccgtg
      721 tatggctcct ccgaatgcgc gcggtaggga tcgagactga gcgaacccag cgagcgcagc
      781 aagtcgccgg agagcgcgag catccggtct ccctggaaga ggatggcgga gtggttctgc
      841 aggcggaact cgaagacgtt ggccgcgagc cccttcccca gggcctcctg aggctccatc
      901 ccctcgacct cgaattccga gccgacggcg atcgcgagcg tccggagctc ctcgtcgatg
      961 tcgcggaacg tgagctcgcg gatcgtcgtc cgggcgccga gcgcgacggc aagcaggagg
     1021 acggccgaga gagcggctcc ggcgagggcg atccgcgtcc tggctcggcg cggccggaga
     1081 aggctcacgt caggcggtat cccactccgc gcacggtctc gattcgagac gacgatccgc
     1141 ccgcttccag tttctttctc aggcgcgcga tgagaacgtc gatcgtgttg ctcatcggct
     1201 cttcccgctc accccacgcg tgttcgagga tcgtttcgcg ggacatcgcg ctgcccgggt
     1261 tccgcatcag cgtttcgaga atggcgaact cacgcgccgt caggaccagc gtctcgtccc
     1321 ggagaagcgc gatgcgccgc gcggggtcga gacgaaggtc gccgctcacg agctcgacgg
     1381 gagcgcgggc ttcgttgcgg cggaggagag cgtgcgcccg ggccgccaga acgtcgaggt
     1441 cgaacggctt accgaggtag tcgtcggcgc cgtcggagag cccctcgacg gtggatgcgg
     1501 gatcgtcgag cgccgtcagc atgagaaccg accgaccgaa gccggcacgc cgggcgcgcc
     1561 gacagacctc gagaccgtcg ccgtcgggga gccgccggtc gaggacgaga aggtcgtact
     1621 cgtgaaggga cagcagctcc tcggcctccg aaatccgcga cgcttcatcg caggcgaggc
     1681 cggcgcgtgt taacgtccga acgaccaggg agcggagccg gaggtcgtcc tcgacgacga
     1741 gagctctcat gacaacgacg tgttcacggc gtattcaccg cggtgattct agtgcccctg
     1801 cggtgagccg gaggggaaag gccggctccc tgctggcgat tgccgtgtcg ctcttcgcag
     1861 gggtcgccgg aggagctccc gagctccgcg cgcaggaagg tccgcggatc cgacggatcg
     1921 tcatcgagac ggaaaacgtc ttccaggacg ccgagtcctc gttcctcgcg agggccgccg
     1981 atgcgattca cggaatcacc catgagcatg tcgtccgccg tgagctcctt tttcaagagg
     2041 gagacacgct ggatccaaag aagctcctcg agacggaacg caatctccgt gcgctcggct
     2101 tcttccgccg ggtggagatc aaggcgacgc cggtcggcga cgatgaggtc gacgtcctcg
     2161 ttcgtacccg ggatgcatgg acgaccgagc tctcgggatc ggtcggtcgg gccggcggcc
     2221 agggacacta cggagtctcg ctcgaagaaa agaacctgtt cggcctgggc aagaaagtgt
     2281 cgtttgcagc tgcccagcgg cccgaccggt cgacacgcga agttctgtac tccgacccgc
     2341 agttcctggg gcgtcgcctc gcgctcgacg tcttctacgg ttcgaacagc gatggagatc
     2401 gccggcactt ctcactcgag agcgggtttc gctcgcttga ctctccgtcg ggagcggttc
     2461 tctcgttcga tcagggcagc cgccagaccc ggctctactc ggaaggaagc gaacttgctc
     2521 ggttcggaat ggacacgcgc tccgtcgaac tttcgggggg acgacggctg actcctgccg
     2581 gagaccgccc cgtgttgcga ctcttcgccg gctaccgccg ggaagaagcg agcttcggct
     2641 cgatcgagca tgctcccgac ggtttcgcgc ttcccgccga ccgcaagttc ggattcttct
     2701 tcgcgcggct cgagctcgcg cacccggagt actccgtgca gcatgacgtg gcgttcttcc
     2761 tgcgcgacga ggacttcgac ctcggcaaca cggcttcgct ggagctcggc tactctccca
     2821 acgtgttcgg cgcagagcaa gcggtgggcg gggcgctcgt gctcgctcgc gggctgcgcc
     2881 tgccgggagg ctttctggtc ggctcgctga gcgcggcgac ccgggcccgc ggaggttcgt
     2941 tcgagaacac tcttgcggaa ggacagcttc tcgcggtctg gcggcccgcc gaaacttccg
     3001 cgaacacgct cgtcgggcgc tttctgttgt cggtgggaaa ccgactcgac cgcgaagtcc
     3061 agctcgaagc cgacggcgca aacggtcttc gagcgtaccg actccacgcg ttcgcggggg
     3121 accggcgcgt gatactgaac ctggagaacc gggtccgcct gaccccggag ctcctccact
     3181 tgatcgtgct cggcgccgct gtcttcgtgg acgccggata tgcgtggcct cccggtgtcc
     3241 cggtccggct ctcggatctc cgggcggatg cgggtgtcgg gctccgcgtc ggtcttcctc
     3301 gcgcttcccg gcacgccctt ttccgcctcg acctcgccta tgcgcttcgg ccggacattc
     3361 aggggcgccg cggctggctc gtgtcgtttt cgagctccca ggccttctga ctccgtcaca
     3421 ggacgatcgt cccgcgcggg cggtgaacgc tcggtgaacg cgggtctata gtctgcgtcg
     3481 ctcgtgactc tgccgacagg atcgcgtctc ggaccctacg agatcctcgc tccgctcggc
     3541 gccggcggga tgggggaggt ctaccgcgct cgcgacagcc ggctcggccg cgacgtcgcg
     3601 ctgaaggtcc ttcctcagga gctctccgag gatcgggatc gcctctcccg tttcgaacag
     3661 gaggcgcgct cggcgtcggc gctgaatcac ccgaacatcg tcaccgtcca cgacatcggc
     3721 cggtcggact cgacctccta catcgcgatg gagctcgtgg acggccgcac gctgcgagag
     3781 ctcgccgccc ccggcccgat gccgacgcgt cggatcctcg ggatcgcggc gcagattgcc
     3841 gaagggctcg cgaaagcgca cgccgcggga atcgtccacc gggacctgaa gcccgaaaac
     3901 gtcatggtgt cgaaggacgg cttcgtcaag atcctcgact tcggcctcgc gaagttggtc
     3961 gagccggagt cgggcgggct ctccgtgatg ccgacgctcg cgaagccgga gacgcacccg
     4021 ggcgtcgtga tgggcaccgt cgcatacatg tcgccggagc aggcgagcgg cgaaccggtc
     4081 gacttccgtt ccgatcagtt ctctctcggc tcgattcttt acgagatcac gagcgggcag
     4141 aggcccttcc agaagaagac cgccgcggaa acgatgtcgg cgatcatccg ggaggaagct
     4201 gatccgatcg gcaaagtccg gcccgaggtt ccgccgccgt tgcgctggat cgtcgagcgc
     4261 tgcctcgcga aggatcccga ggagcggtac gcctccacgc gcgatctcgc ccgggagctc
     4321 gcgagcgtcc gcgaccacgt ctcggaaatc acgggcgcgg cggagccgcc gatcgatctc
     4381 ggaccgcgac ctctgaagag agtccggcgg tccaccgtcc tcgcgacggt cttccttctc
     4441 cttctcttcg gagtcgtcgg aggatggctc gtcgcgtcca agaggcttcc gaagacgtcg
     4501 gcgcccacgt ttcaccgcct gacgttccgc agcggctcgc tccagaacgc gcgcttcacc
     4561 ccggacggcc agaccatcgt ctacggcgcg acctgggcgg gggagaagtc cggtctatac
     4621 gcggtccggc ctgagagtcc ggaatcccgc ccgttcgaga tacccgcgga catactggcc
     4681 gtttcccccg cgggagagat ggcggttctg ctcaatcaga acccgatctc cggaactctc
     4741 gcccgggtcc cgttcgcggg cggagttccc cgtcccatcg tcgagggcgt gccgtacgcc
     4801 agcgccgact ggaccccgga cggaaaagat ctcgtgatcg tgaggtacgt c
//
LOCUS       DASWAV010000579         3605 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U2665
            k141_671872, whole genome shotgun sequence.
ACCESSION   DASWAV010000579 DASWAV010000000
VERSION     DASWAV010000579.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960316
            Sequence Read Archive: SRR10260007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 3605)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3605)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:47:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,929
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3605
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_671872"
                     /isolate="SMAG_U2665"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.36444 N 132.31889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..200
                     /locus_tag="VF958_12055"
     CDS             <1..200
                     /locus_tag="VF958_12055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HEX9149887.1"
                     /translation="LGIADRVGSIEPGKDATLFVSDRDVLDFRYTVVAAYVDGRPLDL
                     SDRHKGLYERYRSRPRPPSAR"
     gene            737..1147
                     /locus_tag="VF958_12060"
     CDS             737..1147
                     /locus_tag="VF958_12060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010048275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EVE domain-containing protein"
                     /protein_id="HEX9149888.1"
                     /translation="MNWLFKEEPTHYSFDDLVSDKKTVWSGVRNPLAQKHLRSVKKKD
                     CVFYYHTGDEKAVVGYAAATSDAYPDPNDPSGKSYVVDIAPIRKLKRPVTLAEIKSKA
                     FFRDFPLVRISRLSVMPVSDREWKEIERMSKAKA"
     gene            complement(1144..>3605)
                     /locus_tag="VF958_12065"
     CDS             complement(1144..>3605)
                     /locus_tag="VF958_12065"
                     /inference="COORDINATES: protein motif:HMM:NF013069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HEX9149889.1"
                     /translation="NAEIPSGRIEGEGREFSVRTRGELVSPEEFAGIFLAQVGDKAVR
                     LSDVADVAVGAEDERTIIRYDKVPAVGLGIVKQASASTLEVANAVKAALPQLNALLPR
                     GMRLEVAYDSSTFIRDSIAEVRMTLLLALGLVVLVIFVFLKSPSATLIPTLAIPVSII
                     GAFTVVYFAGFTVNILTLLALVLAIGLVVDDAIVMLENVYRHLEMGKTRLRAAYDGAK
                     EIGFAILATTIALVAIFIPVAFLTGNVGRLFNEFGMTVAVSVLISGFVALTLTPMLCS
                     KILRGHQTEGRIARTVQRGFDALDRLYDRSVRTALVRRTLVLAITGALIAAILFLFRS
                     LPSELVPTEDRGTIVGIMVAPEGSTLGYVDRYANQIEDIFMAVPERAGLFTGIGLTGE
                     GPGRVTDGFFFLPLKPFSQRDRSQQEIVRELFPRLLAVPGVLAFAINPPSLGGQFSAT
                     PVQYVLQAATYDELRPAVESMMAEASKLPYLLNLDTDLKLNTPQIELAIDRARASALG
                     VSVTDIGTTLQTLLGGRESTQFRRGDKQYKVLLQVRPSERATPSAIQDLYLRGSGGLF
                     QMANVVNARETVAPKELNHFNRVRAARITANLAPGVTLGTALRDLDAIAKAKLPQGVR
                     TDLDGESRELRESSGSLFFLFGISIVFIYLVLAAQFESFQHPLTILLTVPLAVFGALL
                     TLKILGMTLNVYSEIGLILLIGLVTKNAILIVEYANQLRRKEGLEVREAVARAARIRL
                     RPILMTSFATIFGVLPIALGIGAGAESRKPLGVAVVGGLLFSTLLALMVVPVVYTFLS
                     GRVKETETAEEAEVEERAVAG"
BASE COUNT          664 a         1136 c         1217 g          588 t
ORIGIN      
        1 tcctcgggat cgccgatcgc gtcggatcga tcgagccggg gaaggacgcg acgctcttcg
       61 tctccgaccg cgacgtcctc gacttccgct acacggtcgt cgccgcgtac gtcgacgggc
      121 gtccgctcga cctttccgac aggcacaagg ggctgtacga gagatatagg agccgccccc
      181 ggccaccgtc tgcccgatag gtattctcac cttttacggc gcctccggag agcggttttc
      241 ggtagacttt ccttcagcgg aaacccgaaa gtaataccgc gggcgaaggc cgaatcagcc
      301 gggaggtctg gatgaaccca ggtctttcaa cccgggctcg ggtcgtgcgg atgatcggac
      361 tactggcggc gtgctcactt ctctgcgggt gcgatgatgg acccactgcc ccgaccaccc
      421 cagacatttc cggcagctgg cagggaagct atcaaccggg catcgcgagt aatttcgatc
      481 cttgcgatac cgcaggtccg gcggtggcgg cgttcaccca agaggattct cgagtccggg
      541 gaactttgac gacgcagagt cggactttcg tcgagggggc tttcgtcgga gaaatccacg
      601 gcgaccaact tcagggaacc ctgatgaaca acggaacggc gaggaacgtc gcggggtctg
      661 ccagagcggc tcagatcacg ttgagtgtgg atctaccctt ctgctcgatg aaccggatcg
      721 agctccatcg ccagccgtga actggctctt caaggaagaa ccgacccact attccttcga
      781 cgacctcgtc agcgacaaga agaccgtgtg gtcgggcgtg aggaatccgc tcgcccagaa
      841 gcacctgcgc tcggtgaaga agaaggattg cgtcttctac taccacacgg gtgacgagaa
      901 ggccgtcgtc ggctacgcgg cggcgacgag cgacgcctac ccggacccga acgatccgag
      961 cgggaagtcg tacgtcgtcg acatcgcgcc gatccggaag ttgaagcgtc ccgtgacgct
     1021 cgcggaaatc aagtcgaagg cgttcttccg ggactttccg ctggtccgga tctcgcgcct
     1081 ctccgtgatg ccggtctccg accgggaatg gaaggagatc gagaggatgt cgaaagcgaa
     1141 ggcctagccg gcgaccgcgc gctcctcgac ctcggcttcc tccgcggtct cggtctcctt
     1201 cacccgcccg gagaggaagg tgtagacgac cggcacgacc atcaacgcca ggagcgtcga
     1261 gaagagcagg ccgccgacga cggcgacgcc gagcggcttt cggctttcgg ctcccgcgcc
     1321 gatgccgagc gcgatcggca gcacgccgaa aatcgtcgcg aacgaggtca tcaggatcgg
     1381 ccgcagccgg atccgcgcgg cgcgcgcgac cgcctcgcgc acctcgagcc cctctttgcg
     1441 gcgcaactgg ttggcgtatt cgacgatcag gatcgcgttc ttggtcacga ggccgatcag
     1501 caggatgagg ccgatctcgg aatagacgtt cagcgtcatg ccgaggattt tcagcgtcag
     1561 gagggctccg aagaccgcca gtggaacggt caggaggatc gtcagcgggt gctggaagct
     1621 ctcgaactgc gcggcgagca ccagatagat gaagacgatc gaaatcccga acaggaaaaa
     1681 gaggcttccg ctcgactcgc gcagctcccg ggactcgccg tccaggtccg tccggactcc
     1741 ctgcgggagt ttcgccttcg cgatcgcgtc gaggtctcgg agtgcggttc cgagcgtgac
     1801 gcccggcgcg aggttcgccg tgatccgtgc cgcgcgcacc cggttgaagt ggttcaactc
     1861 cttcggcgcg accgtctcgc gcgcgttgac gacgttcgcc atctggaaga ggccgccgct
     1921 cccgcgcagg tagaggtcct ggatggcgct cggcgtcgcg cgctcggagg gccgaacctg
     1981 caggagcacc ttgtactgct tgtcgccgcg gcggaactgg gtcgactccc gaccgcccag
     2041 gagcgtctgg agcgtcgtcc cgatgtcggt gacggagaca ccgagcgcgg aggcgcgtgc
     2101 gcggtcgatc gcgagctcga tctggggcgt gttcagcttc aagtccgtgt cgaggttcag
     2161 gagatagggg agcttcgacg cttccgccat catcgactcg accgcgggcc gaagctcgtc
     2221 gtaggtcgcc gcctgaagga cgtactggac cggggtggcg gaaaactgtc cgccgagcga
     2281 aggcgggttg atcgcgaacg cgagcacgcc gggaaccgcg agcagcctcg ggaacagctc
     2341 gcgaacgatc tcctgctgcg agcggtcgcg ctgcgagaac ggcttcaacg gcaggaagaa
     2401 gaatccgtcg gtcacccggc cggggccttc gcccgtcagg ccgatgccgg tgaagagccc
     2461 cgcgcgctcc gggaccgcca tgaagatgtc ctcgatctga ttcgcgtacc ggtcgacgta
     2521 gcccagggtc gaaccttccg gagcgaccat gatgccgacg atcgttcctc gatcttcggt
     2581 cggcacgagc tcggagggga gcgagcggaa caggaagagg atcgcggcga tgagagcgcc
     2641 cgtgatggca agaacgagcg tgcggcgcac gagcgcggtg cgcacgctgc ggtcgtagag
     2701 gcggtcgagg gcgtcgaagc cccgctggac cgtgcgggcg atccgcccct ccgtctgatg
     2761 cccgcggagg atcttcgagc agagcatcgg ggtcagtgtc agcgcgacga accccgagat
     2821 gagaacggac acggcgaccg tcattccgaa ctcgttgaag agccgcccga cgttgccggt
     2881 caggaaggcc accggaatga agatcgcgac gagcgcgatc gtcgtcgcca ggatcgcgaa
     2941 tccgatttcc ttcgcgccgt cgtacgccgc ccggagtcgc gtcttgccca tctcgaggtg
     3001 tcggtagacg ttttccagca tgacgatcgc gtcgtcgacg acgaggccga tcgcgagcac
     3061 gagagcgagc agggtcagga tgttcacggt gaagcccgcg aagtagacga ccgtgaacgc
     3121 cccgatgatc gacaccggga tcgcgagggt cgggatgagc gtcgcgctcg gactcttcag
     3181 aaagacgaag atcaccagga cgaccagccc cagggcgaga aggagcgtca tgcgaacttc
     3241 cgcgatcgag tcgcggatga acgtcgaaga gtcgtaggcg acctcgaggc gcatgcctct
     3301 cgggaggagg gcgttcagct ggggaagcgc ggccttgacg gcgttcgcga cctcgagggt
     3361 cgaggcgctc gcctgcttca cgatgccgag accgaccgcc gggaccttgt cgtaccggat
     3421 gatcgtgcgc tcgtcttccg cgccgaccgc gacgtcggcg acgtcggaga gtcgcaccgc
     3481 cttgtcaccg acctgggcga ggaaaatccc ggcgaactcc tcggggctga cgagttcccc
     3541 tctcgttcgc accgagaact cccgcccttc cccctcgatg cggccggagg ggatctcggc
     3601 gttct
//
LOCUS       DASWAV010000580         1935 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U2665
            k141_273432, whole genome shotgun sequence.
ACCESSION   DASWAV010000580 DASWAV010000000
VERSION     DASWAV010000580.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960316
            Sequence Read Archive: SRR10260007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 1935)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1935)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:47:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,929
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1935
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_273432"
                     /isolate="SMAG_U2665"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.36444 N 132.31889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1121)
                     /locus_tag="VF958_12070"
     CDS             complement(<1..1121)
                     /locus_tag="VF958_12070"
                     /EC_number="4.1.1.49"
                     /inference="COORDINATES: protein motif:HMM:NF006821.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase"
                     /protein_id="HEX9149890.1"
                     /translation="MATAAVLSPRTIRAKRILKDLTREELRELARTDERTSEYGSPVY
                     STRIKNRSAKNTYVVANGLSIGVMQKPIDRGKAEDTIRAVQERLADTELIQVDRRIGM
                     SPDVPIHCRLYVPREYARIAFMWHNMLFPSNSKKEPDFVSVYVPDWEERLIFLDVDQG
                     YTYILGSDYPGEAKKSMLRQAMYWIKRRGGLGLHAGSKVLRVIGPDGELRDVGFLLFG
                     LSGTGKTTLTLHDHGLQDPERAIIKQDDVVLMTAEGAAYGTEDGFYIKTEGLEPSQTV
                     LWGAATHPTAVYENIKLDENGKVDFLDTSITSNGRGVVLRRFIKGAGEGIDLEKANKI
                     VFITRRNDVVPVCARLTSEQAAAYFMLGESIETSAGDPT"
     gene            complement(1190..1783)
                     /locus_tag="VF958_12075"
     CDS             complement(1190..1783)
                     /locus_tag="VF958_12075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012527806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDIG domain-containing protein"
                     /protein_id="HEX9149891.1"
                     /translation="MSAERQPGRWPSRLEAENVLFEFTRSEALRRHGRAVEETMRAYA
                     GWFGVTDPGEVERWGIVGLLHDFDYEQNPTEDTHLHVGGRILRERGYDEEMVRTIESH
                     ANFMNVPRDTPMKKAIFACDELVGFIGACVKVRPSKSVADLPASSVVKRLKDKAFARS
                     VNRDDVYGGAMEIGRPLEEHIAFTIEALKPIASEIGL"
BASE COUNT          313 a          620 c          606 g          396 t
ORIGIN      
        1 ttcgtcggat cccccgcgga ggtctcgatc gactcgccga gcatgaaata ggcggccgcc
       61 tgctcggagg tcagccgtgc gcagaccggc accacgtcgt ttcttcgggt gatgaagacg
      121 attttgttcg ccttctcgag gtcgatcccc tcgccagcgc ccttgatgaa ccggcgcagc
      181 acgacgcctc ggccattcga agtgatcgag gtgtcgagga agtcgacctt tccgttttcg
      241 tcgagcttga tgttctcgta gaccgcggtc ggatgggtcg ccgcgcccca gagcacggtc
      301 tgcgacggct cgaggccctc ggtcttgatg tagaagccgt cctcagtccc gtaggccgcg
      361 ccttcggccg tcatgaggac gacgtcgtcc tgcttgatga tcgcgcgttc gggatcctgc
      421 aacccgtggt cgtggagcgt cagcgtcgtc tttccagtcc cggagaggcc gaacagcagg
      481 aagccgacgt cgcggagctc gccgtccggg ccgatgactc gcagcacctt cgatccggcg
      541 tggagtccca gtccgccgcg gcgcttgatc cagtacatcg cctggcgcag catcgacttc
      601 ttcgcctcgc ccggatagtc gctcccgagg atgtacgtgt agccctgatc cacgtcgaga
      661 aagatcagcc gctcctccca gtccggcacg tagaccgaaa cgaagtccgg ctccttcttc
      721 gagttggacg ggaagagcat gttgtgccac atgaacgcga tgcgcgcgta ctcgcggggg
      781 acgtaaagcc ggcagtggat gggcacatcc gggctcatcc ctatgcggcg gtccacctgg
      841 atgagctccg tgtcggcgag cctctcctgg actgcccgga tcgtgtcttc cgcctttccg
      901 cggtcgatcg gcttctgcat gaccccgatc gaaaggccgt tggcgacgac gtaggtgttc
      961 ttcgcggacc gattcttgat tcgggtcgag tagacggggg agccgtattc gctcgttcgc
     1021 tcgtcggttc gggcgagctc gcggagctcc tcccgggtca ggtctttcag aattcgcttc
     1081 gcgcggatcg ttcgggggga aaggactgct gccgttgcca tccgttctta cctcctcgtc
     1141 cgcgtggcgg gactaccgaa tctagcagag cctcgcggga ccggtcggat cacaggccga
     1201 tctcgctcgc gatcggtttc agagcctcga tcgtgaaggc gatgtgctcc tcgagcggtc
     1261 ggccgatctc cattgcgcct ccatacacgt cgtcccggtt tacggagcgc gcgaacgcct
     1321 tgtccttcag ccgcttgacg accgacgaag cggggaggtc ggcgaccgac ttcgagggtc
     1381 ggactttcac gcacgcaccg atgaacccga cgagctcgtc gcacgcgaag atcgccttct
     1441 tcatcggcgt gtcgcgcggc acgttcatga agttcgcgtg ggattcgatc gttcggacca
     1501 tctcttcgtc gtagccccgt tcgcgaagga tgcgcccgcc gacgtgcagg tgcgtgtcct
     1561 cggtcgggtt ctgctcgtag tcgaagtcgt gcagcaggcc gacgatgccc caccgctcga
     1621 cctccccggg gtccgtcacg ccgaaccagc cggcgtaagc gcgcatcgtt tcctcgacgg
     1681 cgcggccgtg acggcgcagc gcttcgctcc gggtgaactc gaagagaacg ttctccgctt
     1741 ccagacgcga aggccagcgg ccgggttgtc gttcggcgct catgcgtcga ttctaatgcc
     1801 gccgtttttc gcgcccggtg aacggttgca cacccgagtg tgcaactctg gcagattccg
     1861 atggacaccc tgcagaaaat cggttgattt tcgccaaaat gacactagaa ttctgcaaac
     1921 cgatcgaaaa aaagg
//
LOCUS       DASWAV010000581         4642 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U2665
            k141_589048, whole genome shotgun sequence.
ACCESSION   DASWAV010000581 DASWAV010000000
VERSION     DASWAV010000581.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960316
            Sequence Read Archive: SRR10260007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 4642)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4642)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:47:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,929
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4642
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_589048"
                     /isolate="SMAG_U2665"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.36444 N 132.31889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..476)
                     /locus_tag="VF958_12080"
     CDS             complement(<1..476)
                     /locus_tag="VF958_12080"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX9149892.1"
                     /translation="MRFRDLLRFSVGALRGHHLRTALSLTGVAIGVASVVLLTALGEG
                     ARRYVTGEFASLGSNLLIVFPGKTETTGLSPFVTGAPNDLTLEDADAIARRVPLARRV
                     APIVLGGAAARYGDRSRDVGVWGATADMAIVRKIRLSSGRYLPEGEASRGQRVCVIG"
     gene            complement(473..1189)
                     /locus_tag="VF958_12085"
     CDS             complement(473..1189)
                     /locus_tag="VF958_12085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013034241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX9149893.1"
                     /translation="MIELEGIGRDFIVGEETVHALSGVHLFIPDGDYVSLMGPSGSGK
                     STLLHIIGCLDRPTVGSYRLDGHEVSQLPEEELSRIRRNQIGFVFQFYHLVPRLSAAG
                     NVELPMIFAGIDPKERSRRVAEALETVGLTPRARHRSEQLSGGERQRVAIARAIVMGP
                     KILLADEPTGNLDTTAGAEIVRHLEEGNSRGLTLVVVTHDGDLGGRARHRLQLVDGKI
                     VSEEGALRPVKAAAAGAAAK"
     gene            complement(1186..2388)
                     /locus_tag="VF958_12090"
     CDS             complement(1186..2388)
                     /locus_tag="VF958_12090"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HEX9149894.1"
                     /translation="MSVETTFPLEGRLFSRVTPLWVKRAAWLAAVVVVAVGLKLTLFR
                     PHPVPVTVYRVAAGRVEESVTNSRAGTVKSRRRAELSSEIGGRVAELPAQKGQRVTRG
                     ALLMRVADADYRAQVRVQESAVTAARSTRTEVCRQAELASSELARNRKLAAEAIVSAE
                     ILDQLQSRRDGAVAACESASSRIRQAEAALAVARANLEKTVLKAPFDGIVADVTTEVG
                     EWITPSPPGLPIPPVIVLYDDAETYVSSPMDEVDVAKVHVGQTARVTLDAYPGRSFPA
                     RVKRVAPYVLDVQEQNRTFEIDTELDDAAFARKLPPGTSADVEVILASRDGVLRIPSY
                     AVLEGGRVLVVENRRLVSRSVETGLKNWQFTEVTKGLSVGEGVVVSLDRAEVKEGARV
                     SIAGETAK"
     gene            complement(2385..3509)
                     /gene="amrS"
                     /locus_tag="VF958_12095"
     CDS             complement(2385..3509)
                     /gene="amrS"
                     /locus_tag="VF958_12095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013034474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system radical SAM enzyme"
                     /protein_id="HEX9149895.1"
                     /translation="MSRQMQISDVSQRSAGPIVPTRYWHALEDGRVQCDVCPHFCTLR
                     EGQQGLCFVRARQGDQIVLTTYGRSSGFCVDPIEKKPLHHFLPGTPVLSFGTAGCNLS
                     CKFCQNWDISKSREIEKLSNEASPDQIAMAARELDCRSVAFTYNDPTIFLEYAIDVAR
                     ACHEVGVRTVAVTAGYICREPREEFYRHVDAANVDLKAFTEKFYREVTGAQLQPVLDT
                     LVYLRKETGVWLEITNLVIPGYNDSNEEMDRMTRWIVENLGPDTPVHFSAFHPDWKMR
                     DVPPTPPATLRRVRQIALGNGVRYAYTGNVHDPEGESTFCHGCGDVLIGRDGYDLTDW
                     NLTGEGRCRICDTRLPGVLEEWPGVWNGRRRPVKIGRSAA"
     gene            complement(3484..4500)
                     /locus_tag="VF958_12100"
     CDS             complement(3484..4500)
                     /locus_tag="VF958_12100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020157448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HEX9149896.1"
                     /translation="MLTMSKNGAADLVDPPDTPLVNDVVARRLEEVARLLEAQGADPF
                     RVRAWRSGASRVRRLDRPAEVILREEGTAGLERLPDIGPVISRAIKSLVITGRLPMLE
                     RLRGEMDPEQLLASVPGIGRELAARLHNELDIATLEDLEAAAHSGRLASIEGFGAKRV
                     AGILDTLASRLGRARFPRRTPTEVPVAEILEVDAEYRRKAAAGELTRIAPRRFNPKHE
                     AWLPVFHAERGGRHYTALFSNTARAHELGRTGDWVVIYHDGPDGDQRETVVTVHGGSL
                     SGKRIVRGREEECARFYLDEPRLHSATTVAPGFPVPEDPPWWEKANLGANRGGRDEQT
                     DANQ"
BASE COUNT          738 a         1601 c         1547 g          756 t
ORIGIN      
        1 ccgatgacgc agacccgctg cccgcgggat gcttcacctt ccgggaggta gcggccgctc
       61 gacaggcgga tctttcggac tatcgccatg tcggcggtgg cgccccagac gccgacgtcg
      121 cggctccggt cgccgtaacg cgcggccgct ccgccgagga cgatcggggc cacccgccgg
      181 gcgagaggaa cgcgccgggc gatcgcatcg gcgtcttcga gggtcaggtc gttcggagcg
      241 cccgtcacga acggggagag gccggtcgtc tccgtctttc ccggaaagac gatgaggagg
      301 ttcgaaccga gcgacgcgaa ctcgcccgtg acgtaccgtc gcgcgccctc tccgagggcg
      361 gtcaggagga cgaccgacgc gactccgatc gcaacgcccg tcaacgacag tgccgtgcgc
      421 aggtggtgtc cgcgaagcgc tccgaccgag aagcgaagga gatcgcgaaa cctcatttcg
      481 cggcagcgcc ggctgccgcc gccttcaccg ggcggagcgc tccctcctcc gagacgatct
      541 ttccgtccac gagctggagg cgatgacggg cccgcccgcc gaggtcgccg tcgtgcgtga
      601 ccaccacgag cgtcagcccg cgggaatttc cctcctccag gtgccggacg atctccgcgc
      661 ccgccgtcgt gtcgaggttc ccggtcggtt cgtcggcgag caggatcttc gggcccatca
      721 cgattgctcg ggcgatcgcc acccgctggc gctcgccccc cgagagctgc tcggagcggt
      781 ggcgcgcgcg cggggtgagc cccaccgtct cgagcgcctc cgccacgcgg cggctgcgct
      841 ccttcgggtc gattccggcg aagatcatcg gaagctcgac gttgccggcg gccgagaggc
      901 gcggcacgag atggtagaac tgaaagacga agccgatctg gttgcggcgg atccgggaga
      961 gctcctcctc ggggagctga gagacctcgt gaccatcgag tcggtaggag ccgacggtcg
     1021 ggcggtcgag gcagccgatg atgtggagca gtgtggactt gcccgatccg gaaggtccca
     1081 tgagcgagac gtagtcaccg tccggaatga agagatgaac cccggaaagg gcgtgaacgg
     1141 tctcctcgcc gacgatgaag tcccgtccga ttccttcgag ctcgatcact tcgccgtctc
     1201 gccggcgatc gagacccggg ctccctcctt cacctccgcg cggtcgaggg aaaccacgac
     1261 cccttccccg acggagaggc ccttcgtgac ctcagtgaac tgccagttct tgagcccggt
     1321 ttcgaccgac cgcgagacga gccggcggtt ctcgacgacc agaacgcgcc ctccttcgag
     1381 caccgcgtag ctcgggatcc ggagcacgcc gtctcgcgag gccaggatca cttcgacgtc
     1441 cgccgaagtc ccgggcggga gtttcctcgc gaacgctgcg tcgtcgagct ccgtatcgat
     1501 ctcgaaggtg cggttctgtt cctggacgtc cagaacatat ggcgctacac gcttgacgcg
     1561 ggccggaaac gaacgccccg ggtaggcgtc cagcgtgacg cgtgccgtct ggccgacgtg
     1621 aactttcgcg acgtccacct cgtccatcgg cgacgagacg taggtctccg cgtcgtcgta
     1681 cagcacgatg acgggcggaa tcgggagacc cggcggggag ggcgtgatcc actcgccgac
     1741 ctccgtcgtc acgtccgcga cgatgccgtc gaacggcgcc ttcaacacgg ttttctcgag
     1801 gttcgcccgt gcaaccgcga gcgccgcctc agcctggcgg atccgggaac tggcgctctc
     1861 gcaggctgcg accgcgccat cgcgccgact ctggagttga tcgaggatct ccgccgagac
     1921 gatcgcctcg gccgcgagct tccggttgcg cgcgagctcg cttgacgcga gctccgcctg
     1981 tcggcagacc tccgtgcgcg tcgaacgtgc ggccgtgacg gcgctctcct ggacccggac
     2041 ctgggcccgg tagtcggcgt ccgcgactct catgagcagt gcaccgcgcg tcacgcgttg
     2101 tcccttctga gcgggaagct ccgcgacgcg gccgccgatc tcggaagaga gctcggcccg
     2161 acggcgagat ttcaccgttc ccgcccggct gttggtgacg ctttcctcga cgcgtccggc
     2221 cgccacgcga taaacggtca cggggaccgg gtgcggccga aacagcgtga gcttgagacc
     2281 gaccgcgacg acgaccacgg cggcgagcca cgcggcgcgc ttcacccaca gcggtgtcac
     2341 cctcgagaag aggcggccct cgagcggaaa cgtcgtctcg acgctcacgc ggcggacctc
     2401 ccgatcttca cgggccggcg tcgcccgttc cagacgccgg gccactcttc gaggactccc
     2461 ggcagcctcg tatcgcagat ccggcagcgg ccctcccccg tcaggttcca gtccgtgagg
     2521 tcatatccgt cgcgcccgat caggacgtct ccgcaaccgt ggcagaacgt gctctcccct
     2581 tccggatcgt ggacgtttcc ggtgtaggcg taacgaacgc cgttcccaag cgcgatctgc
     2641 cggacccggc gaagcgtcgc cggcggagtg ggcggaacgt cgcgcatctt ccagtcgggg
     2701 tgaaacgccg agaagtgcac cggagtgtcc ggccccaggt tctcgacgat ccaccgcgtc
     2761 atccggtcca tttcctcatt cgagtcgttg tagccgggaa tgacgaggtt ggtgatctcg
     2821 agccagacgc cggtctcctt ccggagataa acgagcgtgt cgaggaccgg ctgcagctgc
     2881 gccccggtca cctcccggta gaacttctcc gtgaaagcct tcaggtcgac gttcgcggcg
     2941 tcgacgtggc gatagaactc ttcccgcggc tcccggcaga tgtatcccgc cgtcacggcg
     3001 acggtgcgga cgccgacttc gtgacacgcg cgggcgacgt cgatcgcgta ctcgaggaag
     3061 atggtcggat cgttgtacgt gaacgcaacg ctgcggcagt cgagctcacg cgcggccatc
     3121 gcgatctgat cgggtgacgc ttcgttcgag agcttctcga tctcccggga cttgctgatg
     3181 tcccagttct ggcagaactt gcacgagagg ttacatcccg ccgttccgaa cgacagcacc
     3241 ggcgtgcccg gcaggaagtg gtggagcggc ttcttctcga tgggatcgac gcagaagccg
     3301 ctcgagcgac cgtacgtcgt caggacgatc tggtcgccct gcctcgcgcg aacgaaacag
     3361 aggccctgct gcccctcgcg cagcgtacag aaatgcggac atacgtcgca ctggacgcgg
     3421 ccgtcttcca gcgcgtgcca gtaccgcgtc gggacgatcg gcccggcact ccgctgcgag
     3481 acatcactga tttgcatctg tctgctcatc gcgtcctccc ctgttcgcgc cgaggttcgc
     3541 tttttcccac cacggcgggt cttcaggaac cgggaagccc ggcgcgacgg tcgtcgcaga
     3601 gtggagcctc ggctcgtcga gatagaagcg ggcgcactcc tcctcgcgcc cacgcacgat
     3661 ccgctttccg gagaggcttc cgccgtggac ggtgacgacc gtttcgcgct gatctccgtc
     3721 cggaccgtcg tggtagatca cgacccagtc cccggtccga ccgagctcgt gcgcccgagc
     3781 cgtgttcgag aacagcgccg tgtaatggcg tcccccgcgc tcggcgtgga acacgggcag
     3841 ccaggcttcg tgcttcggat tgaagcggcg gggagcgatc cgcgtgagct cgccagccgc
     3901 ggctttcctg cggtactcgg cgtcgacttc gaggatctcg gcgaccggca cttccgtcgg
     3961 cgtccgacgg gggaacctcg cccgcccgag ccggctcgcg agcgtgtcga ggattccggc
     4021 gacgcgcttg gcgccgaatc cctcgatcga ggcgagacgg ccggaatgcg ccgcggcctc
     4081 gagatcctcc agcgtggcga tgtcgagctc attgtggagg cgcgcggcga gttcccgacc
     4141 gatcccggga acggacgcga ggagctgctc cgggtccatc tccccgcgaa gtcgctcgag
     4201 catcggaagg cgccccgtga tcacgaggct cttgatcgcg cgcgagatca ccgggccgat
     4261 gtccggaaga cgttccaggc cggcggtccc ctcctcgcgg aggatcacct ccgcgggccg
     4321 gtcgagccgt cgaactctcg aggcgcccga acgccaggcc cggacgcgga agggatcggc
     4381 gccctgggcc tcgaggaggc gggcgacctc ctcgagccgc cgggcaacca cgtcgttgac
     4441 gagcggggtg tcgggaggat ccaccaagtc ggcggcgcca ttcttcgaca tcgtcaacat
     4501 gactctgctc cacttcgcgc gatcacgcct gatccggttg aatcaaaaga cccttgggga
     4561 cgtcgatcgg ccggaggagg gctgattcaa ttgcatcagt agagtcggtc tcccggcccc
     4621 tacgctcacg acatgaaagg cc
//
LOCUS       DASWAV010000582         2563 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U2665
            k141_589147, whole genome shotgun sequence.
ACCESSION   DASWAV010000582 DASWAV010000000
VERSION     DASWAV010000582.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960316
            Sequence Read Archive: SRR10260007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2563)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2563)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:47:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,929
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2563
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_589147"
                     /isolate="SMAG_U2665"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.36444 N 132.31889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..541)
                     /locus_tag="VF958_12105"
     CDS             complement(<1..541)
                     /locus_tag="VF958_12105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019629032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HEX9149897.1"
                     /translation="MPELEIRKTYKLYVGGDFIRTESGRYDPVPDAKGEFLANVSRGS
                     RKDLREAVRKARAAQPGWASKTQYLKGQILYRMAEMLDGRAESFEKLLRETRGSTAAA
                     ARKEVRAASDRLLHYAGWSDKYGALLGTVNPVASSYFDFSVPEPTGVVGIVAPETPDF
                     LGLVTHLAPVIVSGNSAVVF"
     gene            complement(546..2066)
                     /locus_tag="VF958_12110"
     CDS             complement(546..2066)
                     /locus_tag="VF958_12110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020737994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HEX9149898.1"
                     /translation="MASVKEEEASKSRAIRHVAAEKPALEFGSAWDYSPAPESRDHVR
                     LDSRYGLFIDGKFVAPKSGKYFPTINPATEEPLAEVADANAADVDAAVRAAEDAYRKR
                     WSRLKPAERGKYVYRIARLLQEKSRELAVIETMDGGKPIKESRDVDVPLVVAHFFYHA
                     GWADKLDYAFPRHTVSPIGVCGQIIPWNFPLLMAAWKVAPAIACGNTVVLKPAETTPL
                     TAMALAKVCQEADLPPGVVNIVTGAGETGAAIVNHPRVRKIAFTGSTDVGKIIQRVTA
                     GTGKRLTLELGGKAANIIFEDAPIDQAVEGIVNGIYFNQGHVCCAGSRLLVQESIADV
                     VMRKLSDRIATLRLGDPLDKNTDVGAINSKMQLDKIRELVASGVEEGAEIHQSSCPIP
                     SKGYFFPPTFFTGVAQSHRIAREEIFGPVLSVMTFRTPDEAVEKANNIPYGLSAGVWT
                     DKGSKIFKITSKMRAGVVWANTFNKFDPSSPFGGYKESGFGREGGVHGLRAYVELS"
     gene            complement(2071..>2563)
                     /locus_tag="VF958_12115"
     CDS             complement(2071..>2563)
                     /locus_tag="VF958_12115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017576399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HEX9149899.1"
                     /translation="MVIDRGAFLSGDYEKVASEIVAIKDACGDAHLKVILETGELETY
                     DNVRKASRLAMTYGADFIKTSTGKITPAATPGVVLVMLEAIRDFYFETGRRIGMKAAG
                     GIRTSKQALHLLVLVKETLGDAWLTPNLFRLGASSVTNDLLMQLVKETSGRYQSEDYF
                     TKD"
BASE COUNT          406 a          849 c          847 g          461 t
ORIGIN      
        1 cgaacacgac ggctgaattt ccggagacga tcaccggcgc gaggtgcgtg acgagcccga
       61 gaaagtccgg tgtctccggt gccacgatcc cgacgacgcc cgtcggctcc ggcaccgaga
      121 agtcgaagta cgacgaggcg acggggttca ccgtcccgag gagtgcgccg tacttgtccg
      181 accagccggc atagtggagg agccgatcgc tcgcggcgcg gacttccttg cgcgcggcgg
      241 cggccgtcga accacgcgtc tcgcgaagga gcttctcgaa gctctcggcg cggccgtcga
      301 gcatctcggc catgcggtag aggatctggc ccttcaggta ctgcgtcttc gaagcccagc
      361 ccggctgggc ggcgcgggcc ttgcgcaccg cctcgcgcag gtccttgcgc gagccacgcg
      421 aaacgttggc gaggaactcg cccttcgcgt ccgggacggg gtcgtagcgg cccgactcgg
      481 tccggatgaa atcgccgccg acgtagagct tgtaggtctt gcgaatttcg agctcgggca
      541 tttgctcagc tcaactcgac ataggcgcgc agtccgtgga ccccgccctc gcgaccgaag
      601 ccggactcct tgtagccgcc gaacggcgac gacggatcga acttgttgaa ggtgttcgcc
      661 cacacgacgc cggcgcgcat cttcgacgtg atcttgaaga tcttggatcc cttgtcggtc
      721 cacacgcccg ccgacagccc gtacggaatg ttgttcgcct tctcgaccgc ctcgtccgga
      781 gtccgaaacg tcatcaccga gagcacgggc ccgaagatct cctcgcgcgc gatgcggtga
      841 gactgcgcga cgccggtgaa gaacgtcggc gggaagaagt accccttcga cgggatcgga
      901 caggaggact gatggatctc cgcgccctcc tcgacgccgg aggcgacgag ctcgcggatc
      961 ttgtcgagct gcatcttcga gttgatcgcg ccgacgtccg tgttcttgtc gagcggatcc
     1021 ccgaggcgga gcgtcgcgat gcggtcggag agctttcgca tgacgacgtc cgcgatcgac
     1081 tcctggacga gcaggcggga ccccgcgcag cagacgtgcc cctggttgaa gtagatcccg
     1141 ttgacgatcc cctcgacggc ctggtcgatg ggcgcgtcct cgaagatgat gttggccgcc
     1201 ttcccaccga gctcgagcgt cagccgcttt ccggtcccgg cggtgacccg ctggatgatc
     1261 ttgccgacgt cggtcgaacc ggtgaacgcg atcttgcgga cgcgcggatg gttcacgatc
     1321 gccgcgcccg tctcgccggc accggtcacg atgttgacga cgccgggcgg caggtccgcc
     1381 tcctggcaga ccttcgcgag cgccatcgcg gtcagcggcg tcgtctccgc gggcttcagc
     1441 acgaccgtgt tgccgcaagc gatcgcgggc gcgaccttcc acgccgccat cagcaacggg
     1501 aagttccacg ggatgatctg gccgcagacg ccgatggggg acacggtgtg ccgcgggaac
     1561 gcgtagtcga gcttgtccgc ccaacccgcg tggtagaaga agtgcgcgac gacgagcggg
     1621 acgtccacgt cgcgcgattc cttgatcggc ttgccgccgt ccatcgtctc gatcacggcg
     1681 agctcgcgcg acttctcctg caggaggcgt gcgatccggt agacgtattt cccgcgttcg
     1741 gccggcttca gccgcgacca gcgcttgcgg taagcgtcct ccgcggcgcg gaccgccgcg
     1801 tcgacgtcgg ccgcgttggc gtcggcgact tcggcgagcg gttcctcggt tgccgggttg
     1861 atcgtcggaa agtacttccc cgacttcggc gcgacgaact tcccgtcgat gaagaggccg
     1921 taacggctgt ccagcctcac gtggtcgcgc gattcggggg cgggagagta atcccaggcg
     1981 ctcccgaact cgagcgccgg cttctcggcc gcgacgtgcc ggatcgcgcg ggacttcgaa
     2041 gcttcttctt ccttgacgct cgccatccgt tcagtccttg gtgaagtagt cctcactctg
     2101 gtaccggccg ctcgtttcct tcacgagctg catgagaagg tcgttcgtga cactggacgc
     2161 cccgagtcgg aagagattcg gcgtcaacca tgcgtcgccg agagtctcct tgacgaggac
     2221 gagcaggtgc agagcctgct tggaagtccg gattcccccc gccgccttca ttccaatcct
     2281 ccggccggtc tcgaagtaga aatcgcggat cgcctcgagc atgacgagca cgacgccggg
     2341 cgtcgcggcc ggtgtgatct ttccggtcga tgtcttgatg aagtccgcgc cgtacgtcat
     2401 ggccaggcgc gaagccttcc gaacgttgtc gtaggtctcg agctcgccgg tctccaggat
     2461 caccttcagg tgcgcgtcgc cgcaggcgtc cttgatcgcg acgatctcgg aggcgacctt
     2521 ctcgtagtca ccggagagga acgctccgcg gtcgatgacc atg
//
LOCUS       DASWAV010000583         2295 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U2665
            k141_390256, whole genome shotgun sequence.
ACCESSION   DASWAV010000583 DASWAV010000000
VERSION     DASWAV010000583.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960316
            Sequence Read Archive: SRR10260007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2295)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2295)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:47:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,929
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2295
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_390256"
                     /isolate="SMAG_U2665"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.36444 N 132.31889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(211..465)
                     /locus_tag="VF958_12120"
     CDS             complement(211..465)
                     /locus_tag="VF958_12120"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEX9149900.1"
                     /translation="MMSLMERGGYEVAVYAYREGTEFPPHEHSQDKCDAVLEGSLRIS
                     VGDAAFELGPGDRLYLPARTRHSAAVVGSKTVLSLDGTRW"
     gene            complement(641..931)
                     /locus_tag="VF958_12125"
     CDS             complement(641..931)
                     /locus_tag="VF958_12125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015016399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HEX9149901.1"
                     /translation="MKLYVGNLPYNTTEEDLRNLFSQYGSVDSVAVITDRETGRSKGF
                     GFVEFGNDSEARNAIQALSGQEYGGRALTVNEARPKTAGGGGGGGRPGRDRY"
     gene            complement(1134..2165)
                     /locus_tag="VF958_12130"
     CDS             complement(1134..2165)
                     /locus_tag="VF958_12130"
                     /inference="COORDINATES: protein motif:HMM:NF025697.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4394 domain-containing protein"
                     /protein_id="HEX9149902.1"
                     /translation="MRQTLRFGIGLLTVVFWITPVGAIAETIYAVTSTNLLLTFDSAT
                     PGTAASVAITGLQAGENVLGIDLRPATGQLYALGSTSRLYTINPVTGVATQVGPGPFT
                     PALSGTSFGFDFNPTVDRIRVVSDADQNFRLNPDTGAVVATDTNLAYASGDPNFGTNP
                     NVVASAYTNNVAGATTTTLFGIDSNLDIALIQGSPDGTPISPNTGQLFTALPLFTAGP
                     VLVNTSDAVGFDFSGPPTVGYLSLTPVTGAASSLYTVGAAELTLVGTIGTGFIVVDIT
                     VGVQGPTPTPTATVTATATMTSSPTVTSTAQGGGAAPVPTLSGWALAGMILLLGSLGS
                     FVARGSPSG"
BASE COUNT          403 a          748 c          765 g          379 t
ORIGIN      
        1 tcgattcgga gtcggcgagg tggcagacga cctcgtgcgc cgaccatttc acctcggccg
       61 ggcgccactt catcgcctcg gcgggtacct tcgcgagcgc cgccttgagg cggatgggcc
      121 cctgttcgta tcggcggatc aggtcctcgc gttgctgggg ggtcaaaggc atggaaattc
      181 gcggagtcta ccccgcgacc cgggcggtcc ctaccatcgc gtcccgtcga gggagagaac
      241 tgttttcgaa ccaacgaccg cggcggagtg cctcgtgcga gccggaaggt agagccgatc
      301 gccgggcccg agctcgaagg cggcatcgcc gacggagatg cgcagcgatc cttcgaggac
      361 cgcgtcgcac ttgtcctgcg aatgctcgtg cggaggaaac tccgtcccct cgcggtaggc
      421 gtagaccgcg acctcgtatc ccccgcgctc catcagactc atcatccgtc tctcagtgag
      481 gggtccgtcg gatggatccc agcgctccag acgcactcca accacgggga tcagtctacg
      541 aacccacttc acccgggaaa aagaaaaaag gggggcgaac cccccttgga atgccgtcgc
      601 gcgatcccgg cgaacacgcc gggacagctg cgtcttccgg ttagtaccgg tcccggcccg
      661 gacgaccgcc gccgccgccg ccgcccgcgg tcttcggccg cgcttcgttg accgtcagag
      721 cccgtccgcc atattcctgg ccggagagcg cctggatcgc gttgcgcgcc tcggagtcgt
      781 tgccgaactc cacgaaaccg aagcctttgg agcgaccggt ttcccggtcc gtgatgaccg
      841 ccacggagtc cacgctgccg tactgcgaga agagattccg gaggtcctct tcggtcgtgt
      901 tgtagggaag gttaccgaca tagagcttca tcgaaacgga ttcctgtccc ggcgttcatt
      961 ttgtttcgac ccaccgacgc gccgctcgac ggtgcccgac ctccccgtgg atcgtcggac
     1021 ctcgtcgagc gcgaaactgc gtacggaagg cgagcgagta cgggaagagg aaaaggatac
     1081 cagcttcggc gccgaaagca agggccgacg acgacccctg gccccgggcc agtctagccg
     1141 gagggactgc cgcgcgccac gaaggagccg agagacccga gaagcaggat catccccgcc
     1201 agggcccaac cggacagcgt cggtaccggc gccgctccgc cgccctgcgc cgtcgaggtg
     1261 accgtggggg agctcgtcat cgttgcggtg gcggtgacgg tcgcagtggg cgtgggtgtc
     1321 ggcccctgca ccccgaccgt gatgtcgacg acgatgaatc ccgtcccgat cgtcccgacg
     1381 agggtgagct ccgcggcacc aaccgtatag agagacgaag cggcgccggt gaccggggtc
     1441 aacgagaggt agccgactgt cggcggtccc gagaaatcga aacccactgc atcgctggtg
     1501 ttcaccagga ccgggccggc cgtgaacagc ggcagggccg tgaagagctg gccggtgttc
     1561 ggggagatcg gggttccgtc gggcgacccc tggatcagcg cgatgtcgag gttcgagtcg
     1621 atcccgaaga gggtcgtcgt agtcgccccg gcgacgttgt tcgtgtaggc cgacgcgacg
     1681 acgttcgggt tcgtcccgaa attgggatcg cccgaggcgt acgcgagatt cgtgtcggtg
     1741 gccacgaccg cgccggtgtc ggggttcaag cggaaattct ggtcggcgtc gctgaccacg
     1801 cggatccgat cgacggtggg attgaagtcg aagccgaaac tcgtcccgct caatgcgggc
     1861 gtgaaaggtc cggggccgac ctgcgtcgcc accccggtaa ccgggttgat cgtatagagc
     1921 cggctcgtgc tcccgagcgc atagagctgt cccgtcgccg gtcgaaggtc gattcccagt
     1981 acgttttccc ccgcctgcag accggtgatc gccaccgacg ctgcggtgcc cggggtcgcg
     2041 ctgtcaaacg tcaggaggag gttcgtactg gtgaccgcgt agatcgtttc ggctattgcg
     2101 ccgaccggcg tgatccagaa gacgaccgtc aggagtccga tcccgaagcg caacgtctga
     2161 cgcacgggtc acccccgggg agaaagctgc ccttctactt acgccccgcc cccaggattg
     2221 tcaatctcga agatcctgca gagcgcgcga gacgaacttc ggcgtcatct gttttcgcca
     2281 caggaacgag aagtc
//
LOCUS       DASWAV010000584         2758 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U2665
            k141_233806, whole genome shotgun sequence.
ACCESSION   DASWAV010000584 DASWAV010000000
VERSION     DASWAV010000584.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960316
            Sequence Read Archive: SRR10260007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2758)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2758)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:47:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,929
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2758
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_233806"
                     /isolate="SMAG_U2665"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.36444 N 132.31889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..229
                     /locus_tag="VF958_12135"
     CDS             <1..229
                     /locus_tag="VF958_12135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009748426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HEX9149903.1"
                     /translation="ALGGLAFESCLNVLGEGGAPRPRPKPRFAHGARYEVGGSVLYAS
                     YHPSQQNTFTGKLTQRMLRAVLRQASREAAP"
     gene            complement(235..696)
                     /locus_tag="VF958_12140"
     CDS             complement(235..696)
                     /locus_tag="VF958_12140"
                     /inference="COORDINATES: protein motif:HMM:NF012780.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEX9149904.1"
                     /translation="MKAREIMTADPTTCSREDDLRAVVEIMKSENCGIVPITEGNGDQ
                     KVVGVVTDRDVALYLGKKNVRPSDARASEVMTTDIVSVEPDADVHEVSRKMQQSQVRR
                     VLVTEGKRLLGVVSTADLARASSRSSTDKVGEEVERVMEKVSEDTGSRPRS"
     gene            complement(761..1192)
                     /locus_tag="VF958_12145"
     CDS             complement(761..1192)
                     /locus_tag="VF958_12145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9149905.1"
                     /translation="MNDRKYRHRGYQDSSDEVRPRGPRPGAPGRTEGAPRGRGADQDR
                     EVVFACKSCGEKRRDPEEIRFDSTCSKCGADLHACSQCAHFDTSARFECTQPIPARIP
                     SKKARNSCPLYSPARSFDLTGSRVTETPDDARAAFDRLFKK"
     gene            complement(1217..1846)
                     /locus_tag="VF958_12150"
     CDS             complement(1217..1846)
                     /locus_tag="VF958_12150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019301486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HEX9149906.1"
                     /translation="MTTADLAGATRRATPQPLATIPFPSGTATPSPAAGAPSAFATPA
                     PIPTEAETPPAASLSSAFSTLEGRTLAMPVVGAKTLDLRDNFAEARGARKHEAIDILA
                     ARGTPVVAADEGKVVKLFTSKPGGLTVYQFDRDDTHCYYYAHLDRYAEGLHEGEFLRR
                     GDPIGFVGTTGNAPPNTPHLHFAIFELGPAKHWWEGTAINPYPFLMKMK"
     gene            complement(1960..>2758)
                     /locus_tag="VF958_12155"
     CDS             complement(1960..>2758)
                     /locus_tag="VF958_12155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005994189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HEX9149907.1"
                     /translation="HFSPGEIDGSFGSNTVRTVSGFQEARGLPGTGKIDAATWKALNA
                     DSAPPLEQYAIRPEDVAGPFQQIPEDMMEKSTLPALGYSSSLELLSEKFHASPKLLTK
                     LNPGKSFEKAGEEILVPNVHTEGAGEKAARIVVNKTASTVTAFDREDKPIAQYPATMG
                     SEHDPLPLGDWKVNGVSRNPSFHYNPALFWDADTKDEKATIKPGPNNPVGVVWIDLSK
                     PHYGIHGTPEPSTIGKTQSHGCIRLTNWDAAELAEMVGPGTPAILKE"
BASE COUNT          418 a          895 c          924 g          521 t
ORIGIN      
        1 cgcgctcggc ggcctcgcgt tcgagtcctg cctcaacgtg ctcggtgagg gtggcgcgcc
       61 gcgtccgcgg ccgaagccgc gctttgccca cggcgcgcga tacgaagtcg gcggatcggt
      121 gctctacgct tcgtaccacc cgagccagca gaacactttc accggaaagc tgacgcagcg
      181 gatgctgcgc gcggtcttac ggcaggcgtc gagagaagcc gcaccgtagc gccttcagct
      241 gcgcgggcgg ctgccggtgt cctccgacac tttctccatc acccgctcga cttcttcgcc
      301 gaccttgtcg gtgctcgacc tcgaggatgc gcgtgcgagg tccgcagtcg agacgactcc
      361 gaggaggcgc ttcccttcgg tgacgagcac tcggcgcacc tgagactgct gcatcttgcg
      421 gctgacctcg tggacgtcgg cgtccggctc caccgagacg atgtcggtcg tcatgacttc
      481 cgacgccctg gcatcggacg gccgcacatt tttcttgccg aggtagagcg cgacgtcgcg
      541 gtccgtcacg actccgacga ccttctgatc gccgttgcct tcggtgatcg gcacgatgcc
      601 gcagttctcg ctcttcatga tctcgacgac cgccctcagg tcgtcctccc gcgaacaggt
      661 cgtcggatcg gctgtcatga tctctctggc cttcataaga cgctcctttc ccttgtgggg
      721 acgctctcta tgcaggaagc gttccgccag gccggagcgg ttatttcttg aagagccgat
      781 cgaaggcggc gcgggcgtcg tccggggttt ccgtcacccg cgacccggtc agatcgaagg
      841 atcgggccgg cgagtagagc gggcagctgt tgcgcgcttt cttggacggg atacgcgccg
      901 ggatcggctg cgtgcactcg aaccgggcgg acgtgtcgaa gtgggcgcac tgcgagcacg
      961 cgtggaggtc cgctccgcac tttgaacagg tcgagtcgaa acggatctcc tcggggtccc
     1021 gccgcttttc cccgcacgac ttgcatgcga agacgacttc ccggtcctgg tccgcgccgc
     1081 ggccccgggg agctccctcg gtgcggcccg gcgcccccgg ccgcggcccg cgcggccgaa
     1141 cctcgtcgga ggagtcctgg tatccgcggt gccggtactt gcggtcgttc atcgagggtg
     1201 agggccggcg cccacgttat ttcatcttca tcaggaacgg gtacggattt atcgccgtcc
     1261 cttcccacca gtgcttcgcg ggaccgagct cgaagatcgc gaaatggagg tgcggagtgt
     1321 tcggcggtgc gttgccggtc gtgccgacga agccgatcgg atcgcctcgc cggaggaact
     1381 cgccttcgtg cagaccttcg gcataacgat ccaggtgcgc gtagtagtaa cagtgggtgt
     1441 cgtcgcgatc gaactgatac acggtcaagc cccccggctt gctggtgaag agcttgacga
     1501 ctttcccttc gtcggcggcg acgaccggag ttccccgcgc cgcgagaatg tcgatcgcct
     1561 cgtgcttcct cgctccgcgc gcctccgcga aattgtcgcg gaggtcgaga gtcttcgcgc
     1621 cgacgaccgg catcgcgaga gtccgcccct cgagggtgct gaatgcagag gagagggacg
     1681 cggcgggggg tgtttcggcc tccgttggaa tcggggccgg tgtcgcgaac gcagatgggg
     1741 cgcccgcggc cggggaaggc gttgcggtcc cggaggggaa ggggatcgtc gccagcggct
     1801 ggggcgtggc ccggcgcgtc gcgccggcga gatcggcggt cgtcatcgac gcgaagtggc
     1861 cgggcgccag accacccgtc cgccacagca cgtaatagac agcaaggccg ccgagcagaa
     1921 agccggcgag gaagaggagc gcggcgcgcg cgcggattcc tactccttca ggatggcggg
     1981 agtccccgga cccaccatct ccgcgagctc cgccgcgtcc cagttcgtca ggcggatgca
     2041 cccgtgcgac tgcgtcttcc cgatggtgga gggctcggga gtcccgtgga tcccgtagtg
     2101 cggcttggaa aggtcgatcc agaccacgcc gacggggttg ttgggccccg gtttgatcgt
     2161 tgctttctca tctttcgtgt cagcatccca gaacagagca ggattgtagt gaaaagacgg
     2221 gttcctcgag accccgttga ccttccagtc gccgagtggc agcggatcgt gctcgctgcc
     2281 catcgtcgcg ggatactgcg cgatgggttt gtcctcgcga tcgaacgcag tcacggtcga
     2341 cgcggtcttg ttcacgacga tcctggcggc tttctcgccc gcgccctcgg tgtggacgtt
     2401 cggcacgaga atctcttcgc ccgccttttc gaacgactta cccggattca gtttcgtcag
     2461 cagtttcggg ctcgcgtgga acttctcgga aagaagctcg agcgagctcg agtatccgag
     2521 agcgggaagg gtcgatttct ccatcatgtc ctcgggaatc tgctgaaacg gaccggcgac
     2581 gtcctccgga cggatcgcgt actgctccag cggcggcgcg gagtcggcat tcagtgcctt
     2641 ccacgtcgct gcgtcgatct ttcccgtgcc cggaagaccg cgcgcttcct ggaatccgga
     2701 gaccgtcctc accgtgttcg agccgaacga gccgtcgatc tccccgggag agaaatgc
//
LOCUS       DASWAV010000585         4454 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U2665
            k141_273787, whole genome shotgun sequence.
ACCESSION   DASWAV010000585 DASWAV010000000
VERSION     DASWAV010000585.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960316
            Sequence Read Archive: SRR10260007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 4454)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4454)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:47:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,929
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4454
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_273787"
                     /isolate="SMAG_U2665"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.36444 N 132.31889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2492)
                     /locus_tag="VF958_12160"
     CDS             complement(<1..2492)
                     /locus_tag="VF958_12160"
                     /inference="COORDINATES: protein motif:HMM:NF017044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HEX9149908.1"
                     /translation="MKLRKGLPVLLAALSLLGAAPRKASTVPPPSSQIPKIEFEKYTL
                     PNGLQVILHVDRKLPIVHVNQWYHVGSKNEKPGRTGFAHLFEHMMFQGSKNAAKDYFV
                     YAEKAGANLQEGGVNGTTNNDRTNYFATVPSGNLEEILWLESDRLATLADALTKEKLD
                     NQRDVVKNERRQGLENTPYGRWFKIVTENIHPAGHPYSWTVIGSHEDLTAASLDDVKD
                     FFRTYYTPNNLSLVIAGDFDPAEAKRLVEKYFGGIPAGPPLDRPTLWIPTLDRERIVE
                     ATDRVPQDRVYMTWPSPQIFGPGDAELDLTSLILTDGLSSRLNKVLVYDKQLATAVNS
                     FQNSAEISGWFAVIATARPGAPLAEIERIVTDEIARLGKTGPTMAELERAKTKQRYNF
                     VTGLERIGGFGGKADLLNQYNTFLGDPGRFDEDVARYQKVTAADIQRTVDRWLNTRNR
                     LLVRFHPEKSGRAAEVALDRAKEPAMGADRPFRAPEVKTAKLANGMELYVVERPELPK
                     VAVTLATKAGAIADPAGKAGTAHLTAVTMDLGTKTRKALEIEDALGDLGTELNGVATR
                     ESSRLSFEVLKSNLAAAMAIVSDVVANPTFPQSEFDREKKRHLDALSQQAKNANAIAG
                     RVRSILAFGADHPYGRPVQGLPSSIEGITRADLAEFHETYWKPGSSALIFVGDVTLAE
                     ATDLARKNLAAWSGGAAPAVTIPPAQPATAGKLYLVDRQDAAQTVISPFLPAPPRKTD
                     DYYALHLADAVWGGGGFGTRLNLNLREDKGYSYGVFSNLAHFQQAGLWYAAGGVQTNK
                     TKESLVEFDKELKALAGTKPISDAEFEDAKVKR"
     gene            2625..3581
                     /locus_tag="VF958_12165"
     CDS             2625..3581
                     /locus_tag="VF958_12165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004512089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="KpsF/GutQ family sugar-phosphate isomerase"
                     /protein_id="HEX9149909.1"
                     /translation="MILEAARRVLQIEADSVREQLRNLGPAFEQSVEEIAASVGRICV
                     TGLGKSGLIGQKLAATLSSTGTPAYFLHAAEASHGDLGMLVAGDIVLALTFSGQTPEV
                     ARLIEFARSRNLRSIAMTGEPESLVGRAADLVISVAISREACPLNLAPTASTTAMLAM
                     GDALAMAVSEKRGFREEDFAALHPGGRLGRRFLKVRDLMRTGERVPRVTRDTPMPEAI
                     HEMSRKMMGVTAVVNGDGELAGVISDGDLRRMLESDGALLSKSAGDCLHSNPKTIGAE
                     EFASVALHRMETAKITSLFVVDGEGRLAGAIHMHDLLSAGVA"
     gene            3578..4069
                     /locus_tag="VF958_12170"
     CDS             3578..4069
                     /locus_tag="VF958_12170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013886129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IIIA family hydrolase"
                     /protein_id="HEX9149910.1"
                     /translation="MKRSSSRRIKALFFDVDGVLTDGRLYLDDRGREFKVFDTKDGHG
                     VKMALAAGLKVVWITGRESLATVRRARELGVSHCYPRVTDKEALYRRLCRRWRLEPAE
                     TAAIGDDEPDVPLLNAAGFSACPSDAAPVARKAAQTVLEHPGGRGAVREFVERVLARN
                     GAV"
     gene            complement(4104..>4454)
                     /locus_tag="VF958_12175"
     CDS             complement(4104..>4454)
                     /locus_tag="VF958_12175"
                     /inference="COORDINATES: protein motif:HMM:NF013097.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatC"
                     /protein_id="HEX9149911.1"
                     /translation="LVGMGSNFQAVITINEYLSTASKIILGLGLCFELPIVIFFLTRM
                     GVVSERWLLKKFKYAVLVVFIIAAVITPTPDVATQTVFAVPMLLLYLLGILISWVFRK
                     RRASPREESSSPAV"
BASE COUNT          710 a         1431 c         1510 g          803 t
ORIGIN      
        1 cgcttgacct tggcgtcctc gaactccgcg tcggagatcg gcttcgtgcc ggcgagcgcc
       61 ttcaattcct tgtcgaactc gacgagcgat tccttcgtct tgttggtttg aacgccgccg
      121 gccgcgtacc agagtcccgc ctgctggaag tgcgcgaggt tcgagaaaac accgtaggag
      181 taccccttgt cctcccgcag gttcaaattc aggcgggttc cgaagcctcc gccgccccag
      241 acggcatcgg ccagatggag agcgtagtag tcgtcggtct tccgcggagg cgccggaaga
      301 aacggcgaaa tcacggtctg cgcagcgtcc tgccggtcga cgagatagag cttgccggcg
      361 gtcgccggct gcgccggcgg gatcgtgacc gccggggccg cgccgccgct ccacgcggcg
      421 aggttcttcc gcgcgaggtc ggtcgcctcg gccagcgtca cgtcgccgac gaagatgagg
      481 gcgctcgagc ccggcttcca gtacgtctcg tgaaactctg cgaggtcggc ccgcgtgatc
      541 ccctcgatcg aggaggggag cccctggacc ggacgcccgt acggatggtc ggcgccgaac
      601 gccaggatcg atctcacccg cccggcgatg gcgttcgcgt tcttcgcctg ctgcgagagc
      661 gcgtcgaggt gtctcttctt ctcgcggtcg aactccgact gcgggaaagt cgggttcgcg
      721 acgacgtcgg agacgatcgc catcgcggca gccaggttgc tcttcaggac ctcgaaagag
      781 agtctcgagc tctcgcgggt cgccacgccg ttcaattcgg taccgagatc tccgagcgca
      841 tcctcgatct cgagggcctt gcgggtcttc gtgccgaggt ccatcgtcac ggccgtcagg
      901 tgagcggtgc ccgccttccc cgccggatcg gcgatcgcac ctgccttcgt cgcgagcgtc
      961 accgccacct tcggcagctc cggacgctcg acgacgtaca gctccattcc gttcgccaac
     1021 ttcgcggtct tgacctccgg ggcccggaac ggacggtccg cgcccatggc gggctccttc
     1081 gcccggtcga gggcgacctc ggcggcccgg cccgacttct ccgggtgaaa gcgcacgagc
     1141 aggcggtttc gcgtgttcag ccatcggtcc acggttcgct ggatgtccgc ggcggtcacc
     1201 ttctggtaac gggcgacgtc ctcgtcgaac ctgccggggt cgcccaggaa cgtgttgtac
     1261 tggttcagga ggtcggcctt gccgccgaag cctccgatcc tctcgagccc ggtcacgaag
     1321 ttgtagcgct gcttcgtctt cgcgcgctcg agctccgcca tcgtcgggcc agttttcccg
     1381 agccgggcga tctcgtcggt gacgatcctt tcgatctccg ccagcggggc tccaggacgc
     1441 gcagtcgcga tgaccgcgaa ccagccggag atctctgccg aattctggaa cgagttgacc
     1501 gcggtcgcga gctgcttgtc atacacgaga actttgttca agcgcgagga gagcccgtcg
     1561 gtcaggatga gcgaggtgag atcgagctcc gcgtctccgg gcccgaagat ctgcggagac
     1621 ggccacgtca tgtagacccg atcctgcgga acgcggtcgg tcgcctcgac gatccgttcg
     1681 cggtcgagcg tcgggatcca cagggtcggc cggtcgaggg gaggccccgc ggggatcccg
     1741 ccaaagtatt tctcgacgag ccgcttcgcc tcggcggggt cgaagtctcc ggcaatcacg
     1801 agcgagaggt tgttcggcgt gtagtaagtg cggaagaagt ccttgacgtc gtcgagcgaa
     1861 gcggcggtca ggtcttcgtg gcttccgatc acggtccagg agtacgggtg gcccgccggg
     1921 tggatgttct cggtcacgat cttgaaccag cggccgtagg gcgtgttttc gaggccctgc
     1981 cgccgttcgt tcttgacgac gtcgcgctgg ttgtcgagct tttccttggt gagcgcgtcc
     2041 gcgagcgtcg cgagccggtc cgattccagc cagaggatct cctcgaggtt gccggaaggc
     2101 acggtcgcga aatagttggt ccggtcgttg ttggtcgtgc cgttgacgcc accctcctgg
     2161 aggttcgcgc ccgccttctc ggcgtagacg aagtagtcct ttgcggcgtt cttcgagccc
     2221 tggaacatca tgtgctcgaa gaggtgggcg aagccggtcc gtccaggctt ctcgttcttc
     2281 gagccgacgt ggtaccactg gttcacgtga acgatcggca gcttccggtc gacgtgcagg
     2341 atgacctgca gcccgttcgg aagcgtgtat ttttcgaact cgattttcgg gatctgggag
     2401 gagggaggcg ggacggtgga agccttccgc ggcgccgctc ccaggagcga cagcgcagcg
     2461 agcaagacgg gaaggccctt gcgcaatttc acgggaaact cctttccgga gggcacactt
     2521 cggactcggt tctttcgcgc tttatacgtc agaagacatc gagggttgcc cgcgcgggga
     2581 cggcggacgc aagacgaagg accgaaggga cttaagatag aaaggtgatc ctcgaagccg
     2641 cgcggcgcgt cctccagatc gaggccgact ccgtccgcga gcagctgcgg aacctcggcc
     2701 ccgccttcga acagtccgtc gaggagatcg ccgcttcggt cgggcgaatc tgcgtcaccg
     2761 gcctcggcaa gtcggggttg atcggacaga agctggcggc gacgctctcc tcgacgggga
     2821 cgcccgccta ttttctccac gccgcggagg cgagccacgg tgatctgggg atgctcgtcg
     2881 cgggcgacat cgttctcgct ctcacgtttt ccggtcagac tcccgaagtc gcgcggctga
     2941 tcgagttcgc ccgcagccgc aacctccgat cgatcgcgat gacgggcgag ccggaatccc
     3001 tcgtcggccg cgcggcggac ctcgtgatct cggtcgccat ttcgcgggag gcctgtccgc
     3061 tgaacctcgc gccgaccgcg tcgacgaccg caatgctggc gatgggagac gcgctcgcca
     3121 tggcggtgtc ggagaagcgc ggtttccggg aagaggattt cgcggcgctc catccgggcg
     3181 gacgcctcgg ccgacggttt ctcaaggtac gcgacctcat gcggacgggc gagcgcgtcc
     3241 cgcgcgtcac gcgcgacacg ccgatgccgg aggcgatcca cgagatgtcc cgcaagatga
     3301 tgggcgtcac ggcggtggtc aacggcgacg gcgagctcgc cggcgtgatc tccgacggcg
     3361 atcttcggcg gatgctcgag agcgacggag cgctcctctc gaagtcggca ggcgattgcc
     3421 tacattcgaa tccgaagacc atcggcgcgg aggagtttgc ttcggtcgca cttcatcgga
     3481 tggagacggc gaaaatcacg tcgctcttcg tcgtggacgg cgaaggccga ctcgccggcg
     3541 cgatccacat gcacgatctg ctgtccgcgg gagtcgcttg aagcggtcgt cttcccgacg
     3601 gatcaaagcc ctcttcttcg acgtcgacgg agtcctgacc gacgggcgcc tgtacctgga
     3661 cgaccgcggt cgtgagttca aggtcttcga tacgaaagac ggtcacggcg tgaagatggc
     3721 gctcgcggcg gggctcaagg tcgtctggat caccggtcgc gaatctctcg cgaccgtgcg
     3781 gcgcgcgagg gagctcggcg tctcgcactg ctatccgcgc gtcaccgaca aggaggccct
     3841 ctaccgacgg ctctgccgcc gctggcggct cgagccggca gagaccgccg cgatcggcga
     3901 cgacgagccg gacgtgcccc tcctgaatgc ggccggtttc tcggcgtgtc cgtccgacgc
     3961 cgctccggtc gcgcggaaag cggcgcagac cgtcctcgag catcccggcg gccgcggcgc
     4021 tgtgcgggag ttcgtggagc gggtgctggc gaggaacggc gcggtctgat gaacttctcc
     4081 cgagccgcgt cgtcgccgct gctctagacc gccggggagc tcgactcctc tcggggagag
     4141 gcgcgcctct tccggaagac ccaggagatc aggatcccca ggagatagag aagaagcatc
     4201 ggtacggcga acacggtctg ggtcgcgacg tcgggcgtgg gcgtgatcac cgccgcgatg
     4261 atgaaaacga ccaggaccgc gtacttgaac ttcttcaaga gccagcgctc tgagacgacc
     4321 cccatccgcg tcaggaaaaa gatcacgatc ggcagctcga agcagaggcc gagtccgagg
     4381 atgatcttcg acgccgtcga gaggtactcg ttgatcgtga tcaccgcctg gaagttcgac
     4441 cccattccga cgag
//
LOCUS       DASWAV010000586         2516 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U2665
            k141_433595, whole genome shotgun sequence.
ACCESSION   DASWAV010000586 DASWAV010000000
VERSION     DASWAV010000586.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960316
            Sequence Read Archive: SRR10260007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2516)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2516)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:47:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,929
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2516
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_433595"
                     /isolate="SMAG_U2665"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.36444 N 132.31889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1994
                     /locus_tag="VF958_12180"
     CDS             <1..1994
                     /locus_tag="VF958_12180"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017873293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HEX9149912.1"
                     /translation="DYDRELNDEQRAVVLAGGGPILVIAGAGTGKTRTLVYRVARLIE
                     SGTDPSRIMLLTFTNKAAREMLRRVEALLAVDTRRLMGGTFHSVGNRLLRRFGSRLGL
                     SPNFTILDEEDAREMFEAATSDEGIRTTEKRFPKGDVLLDLYSFTVNTAKTFSEVLTE
                     RAPHFLILEDEIIRVFQRYQQRKRAGNASDYDDLLLSWKRLLAEAPDVAAQLAATYDH
                     VLVDEYQDTNHLQGDIVDGMAKIRRNVAVVGDDAQAIYSFRGASFANILGFPERYPDA
                     KVFRLTLNYRSTPDILALANASIAHNTKQFEKDLRAARPAGPTPAVIPLADVSEQARF
                     IAQRLLEWHDEGDPLSDLAVLYRAHYQALELQIELTRRGIPYEIRSGTRFFEQRHVKD
                     VLAFLKIVVNPKDELSWKRALKIFPRVGERSASAVWAAVASAPDPLAALRSFDPSGLP
                     RGTGAAVAPFTSLVKSLDAPSLRSSPAEAIRYVVESVYRDYARAKFPNGDARLDDLEQ
                     LAQFAQTYESLPGFLEEVTLFNELSGEDVVAGEPSDDRVVLSSVHQAKGLEWSRVVVM
                     WLSEGRFPSYRAAVTEEGLEEERRLFYVAVTRAKNEVALTYPMLARDRYGVDVILEPS
                     RFVTELPEDVYERWTVEVEPGSVVTEEPKEDAPKPETVN"
     gene            2104..>2516
                     /locus_tag="VF958_12185"
     CDS             2104..>2516
                     /locus_tag="VF958_12185"
                     /inference="COORDINATES: protein motif:HMM:NF012708.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HEX9149913.1"
                     /translation="MKADDLYSAICREALESRQRDVMSFVDNGMTGMSARLEKTLIRL
                     PDGHYLLGLGPYNAGLVRLDFDELLIGREVPEFIGVGMPVSGFRVSSPDAFLNTEVSR
                     LHAKLVRRVATDGPHYLLVDMRSTCGTYLNGDPVP"
BASE COUNT          417 a          864 c          823 g          412 t
ORIGIN      
        1 tcgattacga ccgggagtta aacgacgagc agcgtgcggt cgtcctggcg gggggagggc
       61 ccatcctcgt catcgcgggc gccgggacgg ggaagacccg gaccctcgtc taccgggtgg
      121 cgcgcctgat cgagtcgggg accgacccgt cgcggatcat gctcctgacc ttcaccaaca
      181 aggccgcccg ggagatgctg cggcgggtcg aggctctcct cgccgtggac acccggcgcc
      241 tgatgggggg cacgttccac tcggtcggca accgcctcct gcgccgcttc gggagccgcc
      301 tggggctctc cccgaacttc acgatcctgg acgaggagga cgcgcgcgag atgttcgagg
      361 ccgcgacgtc cgacgagggg atccgcacga cggagaagcg ctttccgaag ggggacgtgc
      421 tcctcgatct gtattcgttc accgtgaata ccgccaagac cttctccgaa gtcctgaccg
      481 aacgggctcc gcacttcctg atcctcgagg atgaaatcat ccgcgtcttt cagcgctacc
      541 agcagaggaa gcgcgccggc aacgcgtccg attacgacga cctgctcctc tcgtggaagc
      601 gcctcctcgc cgaggcgccg gatgtcgccg cccagctcgc ggcgacctac gaccacgtgc
      661 tcgtggacga gtaccaggac accaaccacc tgcagggcga catcgtggac gggatggcga
      721 aaatccgcag gaacgtcgcc gtcgttggcg acgacgcaca ggcgatctac tcgttccgcg
      781 gcgcgtcgtt cgcgaacatc ctcggtttcc cggagcgcta tcccgacgcg aaggtcttcc
      841 gcctgacact caactatcgc tcgacccccg acatcctggc gctcgcgaac gcttccatcg
      901 cgcacaacac gaagcagttc gaaaaggacc tccgcgccgc gcgacccgcg ggcccgacgc
      961 ccgccgtcat cccgctcgcc gacgtctcgg agcaggcgcg cttcatcgcg cagcggctgc
     1021 tcgagtggca cgacgagggg gacccgctct cggacctcgc ggtgctctat cgcgcccact
     1081 atcaggcgct cgagctccag atcgagctca cgaggcgcgg gatcccgtac gagatccgat
     1141 cggggacccg cttcttcgag cagcggcacg tcaaggacgt gctcgctttc ctgaagatcg
     1201 tcgtcaaccc gaaggacgag ctctcgtgga agcgcgctct gaagatcttc ccgcgcgtcg
     1261 gggagcggtc cgcttccgcg gtctgggcgg cggtcgcttc ggcgccggac ccgctcgccg
     1321 ccctccgctc cttcgacccg tccggcctcc cgcgcggaac cggcgcggcg gtcgctccgt
     1381 ttacctcgct cgtgaaaagc ctcgacgccc cctcgctgcg ctcgtcgccg gccgaggcga
     1441 tccgttacgt cgtggaatcg gtctaccggg actacgcgcg agcgaagttc cccaacggcg
     1501 acgcgcgcct ggacgacctc gagcagctcg cgcagttcgc gcagacgtac gagtcgctgc
     1561 ccggttttct cgaggaggtc acgctcttca acgagctctc gggcgaggac gtcgtggcgg
     1621 gggagccgtc ggacgaccgg gtcgtccttt cgtcagtcca ccaggccaag gggctcgagt
     1681 ggagccgcgt cgtggtgatg tggctttcgg aggggcggtt cccgagctac cgcgcggccg
     1741 tcaccgaaga agggctcgag gaggagcgcc ggctcttcta cgtcgcggtc acgcgcgcga
     1801 agaacgaggt cgcgctcacc tacccgatgc tcgcgcgcga ccggtacggc gtcgacgtga
     1861 tcctcgagcc gtcccgattc gtgaccgagc ttcccgaaga cgtctacgaa aggtggacgg
     1921 tggaggtgga gccgggaagt gtcgtgaccg aggaaccaaa agaggacgcc ccgaagcccg
     1981 aaacggtcaa ttgacggctc acggaaatcc cgcggctgtg cgaaaatcat tggcagtgac
     2041 gggtttccgg ggacggggaa atctggcatt cgagtcgcag gttgtcccgc gaggacgacg
     2101 gtgatgaagg ccgacgactt gtattccgcc atctgccgcg aagcgctcga gtcccgacag
     2161 cgcgacgtca tgtccttcgt cgacaacgga atgaccggga tgtcggcgcg cctggagaag
     2221 accctcatcc ggcttccgga cgggcactac ctcctcggcc tcggcccgta caacgcgggt
     2281 ctcgtgcgac tcgacttcga cgaactcctg atcggccgtg aggtgccgga gttcatcggc
     2341 gtcggaatgc cggtctcggg gttccgcgtt tcgtcgcccg acgcgttcct gaacacggag
     2401 gtgtcgcgcc tgcacgccaa gctcgtgcgc cgcgtcgcga cggacggacc ccactatctt
     2461 ctcgtcgaca tgcgctcaac gtgtggcacg tacctgaacg gcgaccccgt tccgaa
//
LOCUS       DASWAV010000587         6471 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U2665
            k141_195214, whole genome shotgun sequence.
ACCESSION   DASWAV010000587 DASWAV010000000
VERSION     DASWAV010000587.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960316
            Sequence Read Archive: SRR10260007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 6471)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6471)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:47:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,929
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6471
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_195214"
                     /isolate="SMAG_U2665"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.36444 N 132.31889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..768
                     /locus_tag="VF958_12190"
     CDS             <1..768
                     /locus_tag="VF958_12190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010942137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HEX9149914.1"
                     /translation="EAIDKPYGIVLVTGPTGSGKTNTLYSAIAQLNDPTVNILTAEDP
                     IEFNLAGINQVQMKEQIGLTFASAMRSFLRQDPDIILVGEVRDFETAEIAVKAALTGH
                     LVLSTLHTNDAPSTVNRLMNMGIEPFLVATSINCIAAQRLVRKVCTKCAEDVDTSPQI
                     LIQVGFAPDEVKALKVKRGRGCENCKNTGYRGRIGLFEVLLFSDEIRDMILSGASSIE
                     LKRRAIEEGMVTLRNGGLQKVRDGITTLEEVLRETVV"
     gene            909..2030
                     /locus_tag="VF958_12195"
     CDS             909..2030
                     /locus_tag="VF958_12195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012632054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus twitching motility protein PilT"
                     /protein_id="HEX9149915.1"
                     /translation="MAGEATLHQLLKQMVEMGASDLHLTTNSPPMVRLDGRLLPLPYP
                     PLTVAETKQIAYSVLTDTQKHRFEENLELDFSFGVKGLARFRANNFMQRGAVGGVYRQ
                     IPYQILGFRELGLPPVVELLCEKPRGLVLVTGPTGSGKTTTQAAMIDKINREKQLHIV
                     TIEDPIEFLHTHKKCIVNQRELHADTHSFPNALRSVLREDPDVVLIGEMRDLETIESA
                     LRIAETGHLTLATLHTNSAMSSINRIIDVFPAHQQTQIRTQLSFVLEGILCQSLLERA
                     SGKGRVMAMEILVPNAAIRNLIREDKIHQVYSMMQAGQSKFGMQTFNQSLATLYHRKL
                     ITLEMALSRSSSPDELQELINRGAGVNVPTVAATAGKRS"
     gene            2034..3236
                     /locus_tag="VF958_12200"
     CDS             2034..3236
                     /locus_tag="VF958_12200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005036312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HEX9149916.1"
                     /translation="MPSYAWKGRNRAGMVQEGAFVAESKELALAQLRRQNIVVTGIRE
                     RGKEISLTKVGRKVPPKILAVFTRQFSVMIDAGLPLVQCLEILALQQEHKNFQKILLQ
                     VRQDVEAGSTLADAMRRHPKAFDTLYVNMIAAGEAGGILDTILQRLSVYIEKAVKLRS
                     QVRSALIYPTAVIIIAAIVVAVILLKVIPTFAALFTSLGAELPFPTRMVIAASNFLAR
                     YFIFFVIGFAALAYLIRRYYRTYRGRRVIDGLLLKAPIIGMILRKISVARFCRTLATL
                     TSSGVPILESLDITARTAGNAIVEDAVLETRKSVEGGKTIVEPLKDSNVFPNMVVQMI
                     GVGEQTGSLDAMLNKIADFYEDEVDVAVQGLVKLMEPVMIFILGVIIGGIVIAMYLPM
                     FTLINKIG"
     gene            3257..4972
                     /locus_tag="VF958_12205"
     CDS             3257..4972
                     /locus_tag="VF958_12205"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX9149917.1"
                     /translation="MGDSDRARFISPTSADEADSLARSLGWLSGARIVVFALILLSAV
                     LVQAGSGAVAGISFLYRLLAGAVALSLVHWTAGRFLSPRIDAWVQVLGDLALVTLLVY
                     SSGGPDSVFTFLYLVVIGTAGFLLYRVGAVLTASIAAILYGSMVELVAYGVLPVPPFA
                     SITEWSEPRVRYNLAITVVGLFGVAFMVAYLSEKLVSAREELVRRQKTLARLQHLYAN
                     VIASMSSGLLTTDSRQRVTFLNAPGGEMLGLEPGAITGRNLAELDLPFPCDWASIRKR
                     ARGREPYRAETEFQRGGLRRVLGYSLRVLEGAEGDEGTLILFQDLTDVKKLEGRVRFN
                     EQLAAVGELAAGIAHEIRNPLASISGSVQVLSNELSVGTTERRLMEIIVSESNRLSGV
                     LEEFLRFVRPQERRVANFDVANTISEVMEIFRLSDEVSDAHSIELDVFPDSTALSGDR
                     DQIRQIVYNVAKNAVRAMDAGGRLKVSGREEEGWYVIRFADTGRGMSSDELARLFTPF
                     STAFDGGTGLGMAIVRRIVEDHGGTIDVESRPGEGTTVTIHLPRDARAQHAAQSAAVP
                     MGVAG"
     gene            4969..6411
                     /locus_tag="VF958_12210"
     CDS             4969..6411
                     /locus_tag="VF958_12210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005010973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HEX9149918.1"
                     /translation="MSGQRLLIVDDEASIRDMLAFFFHKRGFEVLTASNFTEGLASSQ
                     RSSPDLILCDIKMPDGNGLDLLRKVKTESPKTPMIMITAHTSTPDAIEAMKAGAVDYI
                     AKPFNIEELGLIVDRALGEKELRDENVYLKQELAAKYTFANIIGKGSRMQEIFRTIER
                     IARVSSTVLLTGESGTGKELIARAIHYTSVRKDRKFVSINCGALPEPLLESELFGHER
                     GAFTGAIKDKRGLFTEADNGTLFLDEISETTPTMQVKLLRAIQEKVIRKVGGNDEVSV
                     DARIIAATNKDLTELVTEGKFREDLFYRINVIPIALPPLRARLEDIAPLTQHFIGKIC
                     KEQKIPEKKISTEAMRLLEAYPWPGNVRELENTLERTVALEPGPVITVSSLPESIALG
                     VRTRVPDFESLPQEGINLESYLEAVGKRLMREALDRCDGVQTKAAELLHMSFRSFRYY
                     AKKYSLVRRDDIYEGEASANGENPLTAEGA"
BASE COUNT         1188 a         2154 c         1975 g         1154 t
ORIGIN      
        1 gaggccatcg acaagccgta cgggatcgtg ctcgtcaccg gtccgaccgg ttccgggaag
       61 acgaacacgc tgtattccgc aatcgcccag ttgaacgatc cgaccgtcaa catcctcacg
      121 gcggaagatc cgatcgagtt caacctcgcc ggcatcaacc aggttcagat gaaagagcag
      181 atcgggctca cgttcgcctc cgcgatgcgc tctttcctgc gccaggaccc ggacatcatt
      241 ctcgtcggcg aggtacgcga cttcgagact gccgagatcg cggtcaaggc cgccctcacg
      301 ggtcacctgg tcctctcgac gctgcacacg aacgacgctc cgtcgacggt caatcggctc
      361 atgaacatgg gcatcgagcc gttcctcgtg gcgacgtcca tcaactgcat cgccgcccag
      421 cgtctcgtac gaaaggtctg cacgaagtgc gcggaggacg tggacacgtc cccgcagatc
      481 ctcatccagg tcggattcgc acccgacgag gtcaaggcgt tgaaggtcaa gcgcggccgc
      541 ggctgcgaga actgcaagaa caccggttac agggggcgca tcgggctctt cgaggtcctt
      601 ctcttctccg acgagatccg cgacatgatc ctctcgggag cctcgtcgat cgagctgaag
      661 cgtcgggcga tcgaggaggg catggtcacc cttcgaaacg gcggccttca gaaggtccgc
      721 gacggcatca cgacgctcga agaggtcctt cgcgaaacgg tggtctagcc ggggtttgcg
      781 gtccatccgg tacgaaaagg cgctccccgc tgccccgcgg cgagccctgg aagcctttca
      841 tttcgcgatg ttcgggctta aactacgacc ttagttcgag acaacccatt tacgagggcc
      901 gtcaaaaaat ggcaggggaa gcgactcttc accagctcct caagcagatg gtcgaaatgg
      961 gggcatccga cctccacctg accacaaatt ctccaccgat ggtccggttg gacgggcggc
     1021 ttctgccgct cccctatccg ccgctgaccg tcgccgagac caagcagatc gcttactcgg
     1081 ttctgaccga cacccagaag catcgattcg aggagaacct cgagctcgac ttctccttcg
     1141 gcgtgaaggg gctcgcgcgc ttccgtgcga ataacttcat gcagaggggc gcggtcggcg
     1201 gagtctatcg gcagatcccc tatcagatcc tgggtttccg cgagctcggt ctgcccccgg
     1261 tcgttgagct tctctgcgag aagccgcgcg gtctcgttct cgtcacgggt ccgaccggat
     1321 cgggcaagac caccactcag gcggcgatga tcgacaagat caaccgggaa aagcagctcc
     1381 acatcgtgac gatcgaggac ccgatcgaat tccttcacac gcacaagaag tgcatcgtca
     1441 accagcgcga gctccacgcc gacacgcatt cgttcccgaa cgctctccgg tcggtcctcc
     1501 gcgaggatcc ggacgtggtc cttatcggcg aaatgcgcga tctggagaca atcgagtcgg
     1561 cgctgcggat cgccgagacc ggccacctca ctctcgcgac cctccacacg aattccgcga
     1621 tgtcctcgat caaccggatc atcgacgtct tccccgcgca ccagcagacg cagattcgca
     1681 cgcagctctc gttcgtgctc gaagggatcc tctgccagtc gctgctcgag cgcgcaagcg
     1741 gaaaggggcg ggtgatggcg atggagatcc tcgtcccgaa cgccgcaatc cgtaacctca
     1801 tccgagagga caagatccac caggtctact cgatgatgca ggcggggcag tcgaagttcg
     1861 ggatgcagac gttcaaccag tcgctcgcga cgctctatca tcgaaagctc atcacactcg
     1921 agatggcgct ctcgcgctcc tccagccccg acgagttgca ggagctcatc aatcgtgggg
     1981 cgggcgtgaa cgttccaaca gtcgccgcga ccgctggaaa gaggagctga caagtgccga
     2041 gctacgcttg gaaggggcgc aaccgcgccg ggatggtgca ggaaggcgcg ttcgtcgccg
     2101 agtcgaagga gctcgcgctc gcgcagctcc gccgtcagaa catcgtcgtc accgggatcc
     2161 gcgagcgggg aaaggagatc tctctcacca aggtcggaag gaaggttccg ccgaagatcc
     2221 tggcggtctt cacccgccag ttctccgtca tgatcgacgc cgggcttccg ctcgtccagt
     2281 gtctcgagat cctcgcgctc cagcaggagc acaagaactt ccagaagatc ctcctccagg
     2341 tgcggcagga cgtcgaggcg ggctcgacgc tcgcggacgc gatgcggcgg catccgaagg
     2401 cgttcgacac gctctacgtg aacatgatcg ccgccggcga ggcgggcggt atcctcgaca
     2461 cgatcctcca gcgtctctcg gtctacatcg agaaggcggt caagctgcgt tcgcaggtcc
     2521 gctccgccct catctatccg acggcggtca tcatcatcgc ggcgatcgtc gtcgcggtca
     2581 tcctcctgaa ggtcatcccg acgttcgctg ctctgttcac gagcctcggg gccgagctgc
     2641 ccttcccgac tcggatggtc atcgccgcct ccaacttcct cgcccggtac ttcatcttct
     2701 tcgtgatcgg cttcgcggcg ctcgcctact tgatccgccg ctattaccgc acctaccgcg
     2761 gccgacgcgt catcgacggc cttctcctga aggcgccgat catcggaatg atcttgagga
     2821 agatttcggt cgcgcgcttc tgccgaacgc tcgcgaccct cacgtcgtcg ggcgtgccga
     2881 ttctcgagag cctcgacatc accgcgcgca ccgccggaaa cgcgatcgtc gaggacgcgg
     2941 ttttggagac ccgcaagagc gtcgagggcg gcaagacgat cgtcgagcct ctcaaggact
     3001 cgaacgtctt cccgaacatg gtcgtccaga tgatcggcgt cggcgagcag acggggtcgc
     3061 tcgacgcgat gttgaacaag atcgccgact tctacgagga cgaggtggac gtggccgtgc
     3121 aggggctcgt caagctgatg gagcccgtca tgatcttcat actcggcgtg atcatcggag
     3181 gaatcgtcat cgcgatgtac ctcccgatgt tcaccctgat caacaagatc ggatagccgg
     3241 cagtctgaga ggcgccgtgg gggactccga ccgcgctcgt ttcatctctc ccacatccgc
     3301 ggacgaggcc gactctctcg cgcgctccct cggctggctc tcgggcgccc gcatcgtcgt
     3361 cttcgcgctc atcctgctct ccgcggtgct cgttcaggcg ggctccggcg ccgtggcagg
     3421 aatctccttc ctctaccggc tcctggccgg cgcggtggcg ctctctctcg tccactggac
     3481 cgccgggcgg ttcctgagtc cgaggattga cgcgtgggtc caggtcctcg gagacctcgc
     3541 cctcgtgacg cttctcgtct actcgtccgg gggaccggac tcggtgttca cgttcctcta
     3601 cctcgtcgtc atcgggacgg ccggcttcct tctctaccgg gtaggcgccg tcctgacggc
     3661 ttcgatcgcc gcgattctct acggctcgat ggtcgagctc gtcgcctacg gagtgctgcc
     3721 cgtgcccccg ttcgcgtcca tcacggagtg gagcgagccg cgcgttcgct acaacctggc
     3781 catcaccgtt gtcggactct tcggtgtcgc cttcatggtc gcctacctgt cggagaagct
     3841 ggtgtccgct cgcgaggagc tcgtccgccg ccagaagacg ctcgcgaggc tccagcatct
     3901 ctacgccaac gtgatcgcgt cgatgtcgtc ggggctgctg acgacggact cgcggcagcg
     3961 ggtcacgttt ctgaacgctc cgggaggaga gatgctcggg ctcgagccgg gagcgatcac
     4021 gggccgcaat ctcgcggagc tcgacctccc cttcccctgt gactgggcga gcattcgcaa
     4081 gcgcgcccgc ggccgtgagc cgtaccgcgc cgaaacggag ttccagcggg gtgggctgcg
     4141 ccgggtcctc ggctattcac tccgagtcct cgaaggcgcc gagggggacg aggggacgct
     4201 cattctgttt caggacctga ccgacgtgaa gaagctcgag ggccgcgtgc ggttcaacga
     4261 acagctcgca gccgttggtg agctggccgc cggcatcgcg cacgagatcc ggaatccgct
     4321 cgcctcgatt tcaggttcgg tgcaggtgct ctcgaacgag ctctcggtcg gaaccacgga
     4381 acgccgtttg atggagatca tcgtctccga gtccaaccgc ctctccggcg ttctcgaaga
     4441 gttccttcgc ttcgttcgcc cgcaggaacg acgcgtcgcg aacttcgacg tcgccaatac
     4501 gatctcggag gtcatggaga tcttccggct gtccgacgag gtctccgatg cgcactcgat
     4561 cgaactcgac gtcttcccgg attctacggc cctgtcggga gaccgcgatc agatccgaca
     4621 gatcgtctac aacgtcgcga agaacgcggt ccgcgcgatg gatgcgggcg gccggctcaa
     4681 ggtctcgggt cgcgaagaag agggctggta tgtcatccgg ttcgccgaca cgggccgggg
     4741 gatgagctcc gacgagctcg cgcggctctt cactccgttc tcgacggcct tcgacggcgg
     4801 caccggactc gggatggcca tcgtccggcg aatcgtcgaa gaccacggcg gcacgatcga
     4861 cgtcgaatcc cgaccgggcg aggggacgac cgtcacgatc caccttccgc gcgacgcccg
     4921 ggcgcaacac gccgcgcagt ccgcggccgt tccaatgggg gtagccggat gagcggacag
     4981 cgccttctga tcgtggatga cgaagcctcg attcgcgaca tgctcgcgtt cttcttccac
     5041 aagaggggat tcgaggttct gaccgcgtcg aacttcaccg aaggcctggc ctcctctcag
     5101 cgctcgtcgc ccgacctcat cctctgcgac atcaagatgc cggacggcaa cgggctcgac
     5161 ctgctgcgga aggtcaagac agagtcgccc aagaccccca tgatcatgat caccgcgcac
     5221 acctcgactc ccgacgcgat cgaagcgatg aaggccgggg cggtggacta catcgccaag
     5281 ccgttcaaca tcgaggagct gggactcatc gtcgaccgcg cgctcggcga aaaggagctg
     5341 cgcgacgaga acgtttactt gaagcaggaa ctcgcggcaa agtacacctt cgcgaacatc
     5401 atcggcaagg gatcccggat gcaggagatc ttccgcacga ttgagaggat cgcgagggtc
     5461 tcatcgaccg tgctcctgac tggcgagagc gggaccggca aggagctcat cgctcgcgcg
     5521 atccactaca catcggtgcg caaggaccgg aagttcgtct cgatcaactg cggagcgctg
     5581 ccggaacccc tgctcgaatc ggagctgttc ggccacgagc gcggcgcttt caccggcgca
     5641 atcaaggaca agcgcgggct cttcaccgag gcggacaacg ggacgctctt cttagacgag
     5701 atctccgaga cgaccccgac gatgcaggtc aagctcctcc gcgcgatcca ggagaaggtc
     5761 atccggaagg tcggaggcaa cgacgaggtc tcggtcgacg cccggatcat cgccgccacg
     5821 aacaaggacc tgaccgagct cgtcaccgaa gggaagttcc gggaggacct cttctaccgg
     5881 atcaacgtca tcccgatcgc gctcccgccg ctgcgcgcgc gcctcgagga catcgccccc
     5941 ctgactcaac acttcatcgg aaaaatctgc aaggagcaga agatcccgga aaagaagatc
     6001 tcgaccgaag cgatgcggct cctcgaagcc tatccgtggc ctggcaacgt ccgcgagctc
     6061 gagaacacgc tcgagcgcac cgtcgctctc gagcccggcc ccgtgatcac ggtctcgagc
     6121 ctgccggagt cgatcgcgct cggggtccgc acccgcgtgc ccgacttcga gagtctgcct
     6181 caggagggca tcaacctcga gagctacctc gaggcggtcg gcaagcggct catgcgcgag
     6241 gccctcgacc gctgcgacgg ggtgcagacg aaggccgcgg agctccttca catgtcgttc
     6301 cgttccttcc gctactacgc gaagaagtac agcctcgtcc ggcgcgacga catttacgaa
     6361 ggggaggcat cggccaacgg cgagaatcct ctcaccgccg aaggggcata agctccgttc
     6421 gtcgatgcgg ggagctcact ctctcgttct cgttctcgcg ctcaccgtct c
//
LOCUS       DASWAV010000588         3429 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U2665
            k141_672327, whole genome shotgun sequence.
ACCESSION   DASWAV010000588 DASWAV010000000
VERSION     DASWAV010000588.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960316
            Sequence Read Archive: SRR10260007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 3429)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3429)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:47:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,929
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3429
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_672327"
                     /isolate="SMAG_U2665"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.36444 N 132.31889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            209..706
                     /locus_tag="VF958_12215"
     CDS             209..706
                     /locus_tag="VF958_12215"
                     /inference="COORDINATES: protein motif:HMM:NF019430.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Spy/CpxP family protein refolding chaperone"
                     /protein_id="HEX9149919.1"
                     /translation="MHPGFIPHWKRQRCGESRVGAGYGPEERRAWRRAWEYEMAGEDD
                     PGSPGFGGGAFGVRRPLRFLAYKLHLEDAQVAALARILDELKTERAQAAVDQRRTLSA
                     FADAVSSDAFDRAKAEAGAKLRLESADRLRDAVVTALGRLHELLNAEQRERLAYLIRT
                     GTLTI"
     gene            complement(751..>3429)
                     /locus_tag="VF958_12220"
     CDS             complement(751..>3429)
                     /locus_tag="VF958_12220"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX9149920.1"
                     /translation="VPAYLEGTAEAVSGIVVRSGYELEILLAETLPIYPAFLPDGRIS
                     IARVSEGDKLVGTGPFCIASQSANRVVLEPNPRYRKETPPRVDRIEFRAGLSASAIAA
                     GFRSGEYDLARDLLPQDREAILREPRFRSGLAETPKKNSYFALFTQSGKAGSNPTLRR
                     ALSDAARAKDFVWGTLGRFALPATGIIPPGILGHDAGRRLPHAAREKAMEMIRSSGVT
                     LPLHLNASVHPILKDQYGALTSSLFESWAELGVEVKIVTADMAAYLESWNEVAGIDVF
                     VGRWIADYDDPDNFTFTLFHSGNGRMRGHFSSPETDEILEEARRESRPGVREALYRKF
                     EGAILESSVLVPLFHDVDYRIAAPAVRGLSLRSTAPFVNYTELGKSAATGPGAVPSPA
                     TAEGVLHVPIAGVLRSLDPSLTETVEQAEVLPNIFETLTLQGEGAVVVPWLASEVIPE
                     NNGMRFRFRLRPGVRFHDGRGLTARDVRYSYERLLQNTKSDSRWQLSPIRGARRLLDG
                     TGTDLEGFHIVSPSEFFIDLEEPVSFFPALLSYEVAAIVPEGTTQIGGTWREGAVGTG
                     PFRAVAFEPGRRLELERNPSYWREGYPKSEAIVFRFAIPPEEIRNDFLSGRLSIASDL
                     LPADAEALRRDPRFASGYRETPRLTTYFVAFNIHRDALRDPAVRRSLVAAVHVPTLVR
                     RTLGSVAIPASGLIPPGLLGYSAGTTSVSASKPPKKSAAASSGEHTVSRETVELIAAV
                     HPVFSGEYAAFFAELSESFREIGFQIRTINQTMAEYIEAQDGRADLSIGRWNADYPDA
                     DTFVYGVLRTREGIIGRMCGTPEIDRLGERGRTEIDPRARHSIYRQIEELVAREALLI
                     PLFHEQIYRFVRPEIEGLQLGISFPTVAYERLSIRR"
BASE COUNT          592 a         1081 c         1214 g          542 t
ORIGIN      
        1 acggcgacag caaggttccg aagccggcag gaccgttgac agatgaactc tatcacgtgg
       61 aaaagagccg ctggcctgca cggaatcggg atttcgtccg gaacttgacc cgccgaacgc
      121 gcgggtctag cctgattttg cgcgtgaggc atgtcgccca cgctcgaggt acccgtcggg
      181 agcgatcgcc ccggcgagag gaggtctcat gcatcccgga ttcattcccc attggaaacg
      241 gcagcgttgc ggcgagagcc gcgtcggcgc gggctatggt cccgaagagc gccgggcgtg
      301 gcggcgggcg tgggaatacg agatggcggg agaggatgat cccggttctc ccgggttcgg
      361 cggcggcgcg ttcggcgtgc ggcggccgct gcggttcctc gcgtacaagc tgcatctcga
      421 ggacgcgcag gtcgcagcgc tcgccaggat cctcgacgaa ctcaagaccg agcgggcgca
      481 ggccgccgtc gatcagcggc gcacgctttc cgcattcgcc gacgctgtct cttcggacgc
      541 gttcgatcgc gcgaaggcgg aggcgggcgc gaagctgcgg ctggagagtg cggatcggct
      601 tcgcgacgcg gtcgtcaccg cgctcgggcg gctgcacgag ctgctcaacg ccgagcagag
      661 agagcgcctg gcgtacctga tccggaccgg aacgctgact atttaggtct tagcgggtca
      721 ggtcttcccg agtggagacc tgacccctgt ctatcgccgg atcgagagcc tctcgtacgc
      781 gacagtcgga aacgagattc cgagctgcag gccttcgatc tcgggccgga cgaaccggta
      841 gatctgctcg tggaagagcg gaatcaggag agcctcacgg gccaccagct cctcgatctg
      901 acggtagatc gagtgccgtg cgcgcggatc gatctcggtc cggccccgtt cgccgagccg
      961 gtcgatctcg ggcgttccgc acatccgccc gatgatcccc tcccgcgtcc gcaggacgcc
     1021 gtagacgaac gtgtcggcgt ccggatagtc cgcgttccag cggccgatcg agaggtccgc
     1081 gcgtccgtcc tgcgcttcga tgtactccgc catggtctgg ttgatcgtgc ggatctggaa
     1141 tccgatctcc cggaacgatt cggagagctc tgcaaagaag gctgcgtact ccccggaaaa
     1201 gaccgggtgc accgcagcga tcaattcgac cgtctcccgg gaaaccgtgt gctctccgga
     1261 actcgcggca gccgacttct tcggcggctt tgaagccgaa accgaagttg tacccgcgga
     1321 gtatcccagg aggccgggag ggatcagccc ggatgccgga atggcgaccg agccgagggt
     1381 ccgacgaacg agagtgggga cgtgaaccgc ggcgacgagg cttcgtcgga cggcggggtc
     1441 cctgagcgcg tcgcggtgga tgttgaaagc gacgaagtag gtcgtgaggc gaggcgtctc
     1501 ccggtagccc gaagcgaacc gcggatcgcg gcggagagct tcggcgtccg ccgggaggag
     1561 gtcggaggcg atcgacagcc ttccggagag aaaatcgttg cggatttcct ccggcgggat
     1621 cgcgaaacga aagacgattg cctcgctctt cggatagccc tcgcgccagt aactcgggtt
     1681 acgttcgagc tcgagccgcc ggccgggctc gaaggccacg gcccggaacg gccccgtgcc
     1741 gacggcgcct tcacgccatg tgccgccgat ctgcgtcgtg ccttccggga cgatcgcggc
     1801 gacctcatag gacagaaggg ccgggaaaaa ggagacgggc tcttcgagat cgatgaaaaa
     1861 ctccgacggc gacacgatgt ggaagccttc gaggtcggtg ccggtgccgt cgagaagcct
     1921 gcgggcgccc cgaatcgggg agagctgcca gcggctgtcg ctcttggtgt tctggagcag
     1981 gcgctcgtac gagtaccgca cgtccctcgc ggtcagcccg cggccgtcgt ggaaccggac
     2041 gcccgggcgc agccggaacc ggaagcgcat cccgttgttc tcgggaatga cttccgatgc
     2101 gagccagggc accacgaccg cgccctcccc ctgcagggtg agcgtctcga agatgttggg
     2161 gagcacctcg gcctgctcga cagtttcggt caacgaagga tccaggcttc gaaggacgcc
     2221 ggcgatcggc acgtggagaa caccctcggc ggtggccggc gagggaacgg cgcccggccc
     2281 ggtcgcggcc gattttccga gctcggtgta gttgacgaag ggagcagtcg accgcagcga
     2341 gagcccccgg actgcgggcg cggcgatccg gtagtcgacg tcgtgaaaga ggggcacgag
     2401 caccgaggac tcgagtatcg cgccttcgaa cttccggtag agggcctcgc ggacgcccgg
     2461 gcggctctct cttctcgcct cctcgaggat ctcgtccgtc tcgggcgagg agaaatgccc
     2521 gcgcatgcgt ccgttgccgg agtgaaacag cgtgaaggtg aaattgtccg ggtcgtcgta
     2581 gtcggcgatc cagcgtccga cgaacacgtc gatcccggcg acctcgttcc aggactcgag
     2641 gtaggccgcc atgtcggccg tcacgatctt cacctcgact ccgagctccg cccagctctc
     2701 gaagagcgac gacgtcagcg ccccgtactg atccttcagg atcgggtgaa cggaggcgtt
     2761 gagatgcagg gggagggtca cgcccgacga gcggatcatc tccatcgcct tttcccgggc
     2821 ggcgtggggc agccgccgcc ccgcgtcgtg cccgaggatg ccgggcggaa tgatccccgt
     2881 cgcgggcagc gcaaaacggc cgagggtccc ccacacgaaa tccttcgcgc gggcggcgtc
     2941 ggacagcgcg cggcggagcg tggggttgga tccggccttg ccggattgag tgaaaagagc
     3001 aaagtaggag ttctttttcg gcgtctcggc gaggcccgag cggaacctcg gctcgcgcag
     3061 gatcgcctcc cggtcctgcg gcaggaggtc gcgggcgagg tcgtactcgc ccgatcggaa
     3121 cccggcggcg atcgcggacg cggataaccc tgcccgaaac tcaatccggt cgacgcgggg
     3181 cggggtctct ttccgatacc tcggattcgg ttcgaggacg acccggttgg cggactgcga
     3241 agcgatgcag aacggcccgg tgccgacgag cttgtctccc tcggagacac gggcgatcga
     3301 gatccgaccg tcgggcagaa aggcggggta gatcggcagc gtctcggcga ggaggatctc
     3361 gagctcgtat ccggaccgaa ccacgatgcc ggagaccgcc tccgccgtcc cttccaggta
     3421 cgcggggac
//
LOCUS       DASWAV010000589         2718 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U2665
            k141_234009, whole genome shotgun sequence.
ACCESSION   DASWAV010000589 DASWAV010000000
VERSION     DASWAV010000589.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960316
            Sequence Read Archive: SRR10260007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2718)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2718)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:47:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,929
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2718
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_234009"
                     /isolate="SMAG_U2665"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.36444 N 132.31889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..922
                     /locus_tag="VF958_12225"
     CDS             <1..922
                     /locus_tag="VF958_12225"
                     /inference="COORDINATES: protein motif:HMM:NF013799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HEX9149921.1"
                     /translation="HLGRNPSGAPDLLFVGLSSLDYYAHRAGPDSLEVADGVVRLDAQ
                     LETFFRWLDSEIGANRTLIFLTADHGLTPIPEVARAKARPRSGKEDPNLAGRVDFGNA
                     RGSGAAVRDGAPDRLALETHLAAVFGYTLDPALPNALEGAVLRFEDPIGFYLNRPVIA
                     RRRLPPEKVKEAVRDWVRGRPGVRVAYTNTEVADGLPATEPLALAIERSFRADRSADV
                     VVYLKPGWIFRKEPGSTHGEPTDDDSRVPLLAWGPGVRVGSWDLRASPLSIARTVGAL
                     FGFEAGEPDAEVLQPVLGRDEGTRAPAVPP"
     gene            919..1317
                     /locus_tag="VF958_12230"
     CDS             919..1317
                     /locus_tag="VF958_12230"
                     /inference="COORDINATES: protein motif:HMM:NF013936.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043169 - cation binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HEX9149922.1"
                     /translation="MTVAETLTQEHRLLDDLFGRFLASPDRESAARAIREFDATLRRH
                     TEHEEEHVFPAAAGGKLVARAGESDGDRLFRELRVEHVQIRELSGMIVRQLSEAGEGG
                     AVRGLAANLARRWDAHSAREEREAFAFLTG"
     gene            complement(1332..>2718)
                     /locus_tag="VF958_12235"
     CDS             complement(1332..>2718)
                     /locus_tag="VF958_12235"
                     /inference="COORDINATES: protein motif:HMM:NF015557.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HEX9149923.1"
                     /translation="RVLMPLAFASILAGSVTLIGTSTNLVISSQLPRYGLRPIGFLEL
                     APVGLGITVVGLLYLFFLAPRLVPDRGEREMTDRYDLRRYLSEALIQPGSRLAGKSLE
                     ESQLGSAMNLNVVGIMRGKRRTLAPGPHATLAEGDILIIEGKAEDILSVKDAGGIEIR
                     PDVKPALSDGDLQSEQIRMKEAMVLPQSRFAGRTLKESQFREQTGLTVLAIHSAGGPD
                     RIEKLSGIRVKASDVLLLQGTVEALERLRERRDLLILEDVSAHHPKRRKARRAAVIFA
                     VSILAGVTGLVPLVLAFIGGGVLLVVTECMTPQEAYESVDWRLMVMIACMISFGLAME
                     KTGTAAFLSELIVRHVSGLGSTALLAAFFFFTVLLTQPMSNQAAALVVLPVAIGVATR
                     LGLNPRSFVMCVTFAASCSFLTPLEPSCVLVYGPGRYRFFDFARVGLPLTALVFVLSM
                     LLIPVVWPLGR"
BASE COUNT          483 a          917 c          929 g          389 t
ORIGIN      
        1 ccacctcggg cgaaacccgt cgggcgcgcc ggacctgctg ttcgtcggcc tctcctccct
       61 cgactattac gcgcaccgcg ccggacccga ctcgctcgaa gtcgcagacg gcgtcgtccg
      121 cctcgacgcg cagctcgaga cgttcttccg gtggctggac tcggagatcg gcgcgaaccg
      181 cacgctgata ttcctgaccg ccgaccacgg gctgacgccg atccccgagg tcgcgcgcgc
      241 taaggcgcga ccgcgctcgg gaaaggaaga tccgaacctc gcgggacgcg tcgacttcgg
      301 aaacgcgagg ggctccggcg cggcggtgcg ggacggcgcg cccgaccgcc tcgctctcga
      361 gacgcacctc gccgccgttt tcggatacac gctcgacccg gcgcttccga acgcgctcga
      421 gggggccgtg cttcggttcg aagatccgat cggcttctat ctgaaccgcc cggtgatcgc
      481 gcggcgccgg ctgccgcccg agaaggtgaa ggaagcagtc cgcgactggg tccgcgggcg
      541 acccggagtg cgggtcgcgt acacgaacac cgaggtcgcc gacggcctcc ccgcgaccga
      601 gccgctcgcg ctcgcgatcg agaggtcctt tcgcgcggac cgttcggccg acgtcgtcgt
      661 ttacctgaag ccgggctgga ttttccggaa ggaaccgggc tcgacgcacg gcgagccgac
      721 cgacgacgac tcccgggtgc cgcttctcgc gtgggggccg ggagtgcgcg tgggttcatg
      781 ggacctgcgc gcgtcgccgt tgtcgatcgc gcggaccgta ggagctctct tcggattcga
      841 agcgggcgag ccggacgcgg aggttctcca gccggtcctc ggccgcgacg agggaacgcg
      901 cgctccggcg gtccctccgt gacggtcgcg gagaccctga cgcaggaaca ccgcctcctc
      961 gacgatctct tcggacggtt ccttgcgtcg ccggaccgcg agagcgccgc tcgcgcgatc
     1021 cgggagttcg acgcgacgct ccgccgccac acggagcacg aggaggagca cgtcttcccc
     1081 gccgctgccg gggggaagct cgtcgcgcgg gccggcgaat cggacggaga ccgcctcttc
     1141 cgcgagcttc gcgtcgagca cgtccagatc cgggaactct cggggatgat cgtgagacag
     1201 ctcagcgagg ccggcgaggg aggcgccgtg cgcggtctcg cagcgaacct cgcccgccgg
     1261 tgggacgcgc acagcgcgcg cgaagagcgc gaggcgtttg ctttcctgac agggtgaccg
     1321 ggccccggaa cctatcttcc gagcggccac accacgggaa tcagcagcat cgagaggacg
     1381 aacaccagcg cggtcaacgg gagccccacg cgtgcgaaat cgaagaaccg atagcgcccc
     1441 ggcccgtaga ccagaacgca cgacggctcg agcggcgtca ggaacgagca cgatgccgcg
     1501 aaggtcacgc acatgacgaa agagcgcgga ttcagcccga gcctggtggc gacgccgatc
     1561 gcgaccggaa gaacgacgag cgcggcggcc tgattcgaca tcggctgcgt caacagtacg
     1621 gtgaagaaga agaaggcggc gagcagcgcc gtgctgccca gaccgctgac gtgacgcacg
     1681 atcagttccg aaaggaacgc cgccgtgccc gtcttctcca tcgcgaggcc gaaagagatc
     1741 atgcaggcga tcatgaccat gaggcgccag tccacggact cgtaagcctc ctgcggggtc
     1801 atgcattcgg tcactacgag caggaccccg ccgccgatga aagcgaggac cagcggaacg
     1861 agcccggtca cacccgcgag gatcgagacg gcgaagatga cggctgcgcg gcgggccttg
     1921 cggcgcttgg ggtgatgcgc cgagacgtcc tcgagaatca gaaggtctct ccgttcccga
     1981 agccgttcga gcgcctcgac ggtgccctgg agcagaagaa cgtcgctcgc tttcacccgg
     2041 atgccggaca gcttttcgat ccggtccgga ccgcccgcgg aatgaatcgc caggacggtc
     2101 aatcccgtct gctcgcggaa ctgagactcc ttgagcgtcc tgccggcgaa tcgggattgc
     2161 ggaaggacca tcgcctcctt catgcggatc tgctcggact ggaggtcacc gtccgagagc
     2221 gccggcttca cgtcgggacg aatctcgatc ccgcccgcgt ccttgacgga aaggatgtcc
     2281 tccgcttttc cctcgatgat caggatgtcg ccctccgcaa gagtggcgtg tggcccgggc
     2341 gccagagtcc ggcgcttgcc gcgcatgatc ccgacgacgt tcaggttcat ggcggagccg
     2401 agctgactct cctcgagcga cttgcccgcc agacgggacc ccggctgaat cagggcctcg
     2461 gagagataac gccgcaggtc gtaacggtcg gtcatctcac gctcgccgcg gtccggaacc
     2521 agccgcggag cgagaaagaa aaggtagagc aatccgacga cagtgattcc cagtcctacc
     2581 ggcgcgagct cgagaaagcc gatcgggcgg agcccatagc gcggcaactg actcgagatc
     2641 acgagattcg tggacgtccc gatgagtgtc acgcttcccg cgaggatcga ggcgaatgcg
     2701 agcggcatga gtacgcgg
//
LOCUS       DASWAV010000590         3919 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U2665
            k141_33962, whole genome shotgun sequence.
ACCESSION   DASWAV010000590 DASWAV010000000
VERSION     DASWAV010000590.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960316
            Sequence Read Archive: SRR10260007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 3919)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3919)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:47:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,929
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3919
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_33962"
                     /isolate="SMAG_U2665"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.36444 N 132.31889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..772
                     /locus_tag="VF958_12240"
     CDS             <1..772
                     /locus_tag="VF958_12240"
                     /inference="COORDINATES: protein motif:HMM:NF019869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HEX9149924.1"
                     /translation="VNRHLTAFRFDEAAQAIYGFLWHELCDGYLEMVKPVLSGREGDE
                     SAREAARGVLYRCLADSLALLHPFMPFLTEEIWEKLTRRPGTLIVSRYPAGDPALEDA
                     RAEAAVERLRALVTRVRNFRGERRVSPTEPVHLWLDMDAEVPAAAQDLHGLERQLRHL
                     ARLSELTFAPAPAGAARDVVSGVAVGLALPAGAAEADRSRAGKTLAQLEKEIGELSGK
                     LQNPAFLDKAPADVVEKTRRRLVELEERRAALGDVRPE"
     gene            769..1572
                     /locus_tag="VF958_12245"
     CDS             769..1572
                     /locus_tag="VF958_12245"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR00121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004077 - biotin-[acetyl-CoA-carboxylase]
                     ligase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HEX9149925.1"
                     /translation="MSRPSATSPALLQFANGTLEPGRWTTFENVACLSSIPSSNALAR
                     ELVDFYLQEEQFLPPTLLVAEEQPFARGRSGRQWKAPRGRGLYFTCVRRIEEGEPLSI
                     VPIAAARWLRDVIARVCGLEAGVKWPNDVYVGRRKLAGVLSEACTQGEDSYLAVGIGI
                     NVLGTAAELGVPDATTLEQETGRRFGLAQLLQSVVDRLDRGFAQPDWAQEVEEWQSVA
                     VHRPGDRMRVRADGDVVAGEYRGLDPSGFLRLVTDTGETIVSSGEVAEW"
     gene            1566..2357
                     /locus_tag="VF958_12250"
     CDS             1566..2357
                     /locus_tag="VF958_12250"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012772073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III pantothenate kinase"
                     /protein_id="HEX9149926.1"
                     /translation="MVTESAGRLLAVDVGNTNTVLGLFEGRDLVAHWRLTTRREATSD
                     EITLSVRGLLSSVGRGGEVSCDVIVASVVPSLRFPMKQALRQITDREPLFVEPGIRTG
                     MPILYDIPQEVGADRIVNAVAAHSRLGGPCIVVDFGTATTFDVVTARGEYLGGVIVPG
                     ISISAEALFEKAARLWRVEIRRPDRVIGKTTAASIQSGLYFGYLSLVDGLIDRISNEI
                     GARPRVIATGGLAELFGSASEKIEAVDPLLTLTGLLLIYERNRGG"
     gene            2335..3006
                     /locus_tag="VF958_12255"
     CDS             2335..3006
                     /locus_tag="VF958_12255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9149927.1"
                     /translation="MSETEEDRAYYAAGEAAFIARRGTPFLLSPKDFALLKEWRALGV
                     PIEAVEAGIDDAFTRREERRGTGRINSLSYCRDAVLSAWERRSETAVGSGRGREETET
                     TDAGARLARLAEALDSVARLHPELAERLDSAHRSLDRLASSRKTPGEIETSLARLDRR
                     LANELSDALPAARRNRVESRVEELLARARLKMDRETEEKTRRALTRRTLREELALPRL
                     TLLGG"
     gene            3127..>3919
                     /locus_tag="VF958_12260"
     CDS             3127..>3919
                     /locus_tag="VF958_12260"
                     /inference="COORDINATES: protein motif:HMM:NF014047.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9149928.1"
                     /translation="MSRGEHGVGFIAGALPGEEVEAEIDEIRSTFWRGRTVEVLSASQ
                     DRVLSPAEACPGCDWGHFEPSAARQAKRALFLETMQRIAKLRPDAFGELPIAPSPLRY
                     RIRNRFHLEGRGADLVLGQFAGRTHHVESVGGCRALTEQTAAILAAVREALAGSGAAA
                     SEFATLEDPAGERRLARVALGDAAKRHGRSHAEAVSDALSTFFAGVKVVDGQGTLLRE
                     AGEPRLAIATGARVFQVSVDSFFQGNRHLAGRVLTDVAEATAGPPG"
BASE COUNT          582 a         1344 c         1370 g          623 t
ORIGIN      
        1 cgtgaaccga cacctgaccg catttcgctt tgacgaggcg gcgcaggcga tctacggatt
       61 tctctggcac gagctttgtg acggttacct ggagatggtg aagccggtgc tctccggccg
      121 cgagggggac gagagcgccc gcgaggcggc tcgcggggtg ttgtaccggt gcctcgcgga
      181 ttcactcgcg ctccttcatc ccttcatgcc cttcctgacc gaggaaatct gggagaaact
      241 gacccgacga ccggggacac tgatcgtttc gcggtatccc gcgggggacc ccgcgctcga
      301 ggacgcccgc gcggaggcgg cggtcgagcg cttgcgggcg ctcgtcaccc gcgtccgcaa
      361 cttccgcggc gagcggcgcg tgtcgccgac cgagcccgtg catctgtggc tcgatatgga
      421 cgctgaggtc ccggccgccg cgcaggatct gcacggactc gagcgacagc tccgccacct
      481 ggctcgcctc tcggaactca cgtttgctcc ggcgccggcc ggtgcggcgc gcgacgtggt
      541 gtccggcgtc gcggtcggtc tggcgctgcc cgcgggtgcc gctgaggcgg accgctcccg
      601 cgccgggaaa accctcgctc agctcgagaa ggagatcggc gaactttccg ggaagctgca
      661 gaatccggcc ttcctggaca aggcgcccgc cgacgtcgtc gagaaaacgc gacgccgtct
      721 cgtcgagctc gaagagcgcc gcgccgctct cggcgacgtc cgcccggaat gagccgcccg
      781 tcggcgacga gcccggcgct tctccagttc gccaacggaa ctctggaacc gggccgctgg
      841 accacgttcg agaacgtcgc ctgcctctcc tcgatcccat cctccaatgc gctcgcccgg
      901 gagctcgtcg acttctatct ccaggaggag cagttcctcc cgccgacgct cctcgtcgcg
      961 gaggagcagc ccttcgcgcg cgggcgaagc ggacggcagt ggaaggcccc gagggggcgc
     1021 ggcctctatt tcacgtgcgt gcgccggatc gaggaggggg agccgctttc gatcgtgccg
     1081 atcgccgcgg cgagatggct ccgcgacgtg atcgcccggg tgtgcggcct cgaagctggc
     1141 gtcaagtggc ccaacgacgt ctacgtcggg cgccggaaac tcgcgggggt tctctcggaa
     1201 gcgtgcaccc agggggagga ctcgtatctg gccgtcggca tcgggatcaa cgttctcgga
     1261 accgcggcag agctcggggt ccccgacgcg accaccctcg agcaggagac ggggcgcagg
     1321 ttcgggctcg cacagctgct ccaatcggtt gtcgatcggc tggatcgcgg tttcgcacag
     1381 cccgactggg cgcaagaggt cgaagaatgg cagagcgtgg cggtgcaccg gcccggagac
     1441 cggatgcggg tgcgcgccga cggcgacgtg gtcgccggag agtaccgggg gctcgatccg
     1501 tcgggcttcc tccggcttgt cacggacacg ggggagacga tcgtgtcgag cggcgaggtg
     1561 gcggaatggt gacggagagc gcgggccggc tcctggcggt ggacgtcggc aacaccaaca
     1621 ctgtgctcgg cctgttcgag gggcgcgacc tcgtcgccca ctggaggctg accacccgcc
     1681 gcgaggcgac ctcagacgag atcacgctgt cggtccgggg actgctgtcc tccgtggggc
     1741 ggggaggcga ggtctcgtgc gacgtcatcg tcgcgagcgt cgttccttcc ctccgattcc
     1801 cgatgaaaca ggcgctgcgc cagatcaccg atcgcgaacc gctcttcgtc gagccgggca
     1861 tccggaccgg catgccgatc ctctacgaca tcccgcagga ggtcggagcc gaccggatcg
     1921 tcaacgccgt ggcggcgcac tcgcgcctcg gaggcccctg catcgtcgtc gacttcggga
     1981 ccgccacgac gttcgacgtc gtcaccgcac gcggcgaata cctgggcggc gtgatcgttc
     2041 ccgggatctc gatatccgcg gaagctctgt tcgagaaggc cgcgcgcctg tggcgcgtcg
     2101 agatccggcg cccggacagg gtgatcggca agacgactgc cgcctccatt caatcgggcc
     2161 tctacttcgg gtacctctct ctcgtcgacg ggttgatcga ccgcatctcg aacgagatcg
     2221 gcgcacggcc ccgggtcatc gccacgggcg gcctcgccga gctcttcggg tccgcctcgg
     2281 agaagatcga ggccgtcgat ccgctgctca cgctcacggg actcctcctg atctatgagc
     2341 gaaaccgagg aggatagggc ctactacgct gcaggagagg ccgccttcat cgcgcgccgc
     2401 ggaacccctt tcctcctgtc gcccaaggat ttcgcgctac tgaaggagtg gcgcgcgctc
     2461 ggcgttccga tcgaggccgt cgaggcggga atcgacgacg ccttcacgcg acgggaagag
     2521 cgtcgaggga ccggacggat caactccctg tcgtactgcc gcgacgcggt gctctccgca
     2581 tgggaacgcc gctcggagac cgccgtggga agcggacggg gccgcgagga gaccgaaacg
     2641 accgacgccg gcgcgaggct cgcccggctc gcggaagccc tcgactcggt cgcgcggctc
     2701 cacccggagc tcgccgagcg gctcgattcc gctcaccggt cgctcgatcg gctcgcgtcc
     2761 tctcgcaaga cccccggcga gatcgaaacg tcgctcgcgc gcctcgaccg gcgcctggcg
     2821 aacgagctct ccgacgccct tccggccgcc cgccgcaacc gggtcgagtc gcgcgtcgag
     2881 gagctcctcg cgagagcgcg cctcaagatg gaccgggaga ccgaggagaa gacgcgccgc
     2941 gccctcacgc ggcgcactct gcgggaagag ctcgctcttc cccgcttgac gttgcttggc
     3001 gggtgaaaga tttttctgtc gttttccttt tcccgttttc cgattcccga caataagtag
     3061 agaggcccgc cgatcgcttc ccgtcgccgc atccgcattt cccggctcgc tccaacaggc
     3121 gagggaatct cgcgtggcga gcatggcgtc gggttcatcg ccggagcttt gccgggggag
     3181 gaggtcgagg cggaaatcga cgaaattcgc agcacgttct ggcgcggccg gacggtggaa
     3241 gtgctctccg cgtcgcagga ccgggtgctc tctcccgcgg aggcctgccc cggttgcgac
     3301 tggggccact tcgagccttc ggccgctcga caggcgaaac gggccctctt cctcgaaacg
     3361 atgcagagga tcgcgaaact ccgcccggac gccttcggcg agctcccgat tgcgccttcg
     3421 cctctgcgtt atcgaatccg caatcggttt cacctcgagg gccgcggggc ggacctcgtg
     3481 ctcggccagt tcgcagggcg gacgcaccac gtcgagtcgg tcggagggtg ccgggcgctc
     3541 acggagcaga ctgcggcgat ccttgcggcg gtgcgcgaag cactcgccgg gagcggcgcc
     3601 gcggcgtcgg agttcgccac actcgaagac cctgcgggcg agcggcgcct cgcccgtgtc
     3661 gcgctcgggg acgcggcgaa gagacatggg cgctcccacg cggaggcagt ctccgacgct
     3721 ctctccacgt tcttcgccgg tgtgaaggtc gtggacgggc aggggacgct ccttcgcgag
     3781 gcgggcgagc cgcgtcttgc gatcgcgacg ggcgcccggg tcttccaggt ctcggtcgac
     3841 tcgttctttc agggaaaccg ccatctcgcg ggccgggtgc tgacagacgt cgcagaagcg
     3901 actgcgggcc ccccgggcg
//
LOCUS       DASWAV010000591         7998 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U2665
            k141_195227, whole genome shotgun sequence.
ACCESSION   DASWAV010000591 DASWAV010000000
VERSION     DASWAV010000591.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960316
            Sequence Read Archive: SRR10260007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 7998)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7998)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:47:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,929
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7998
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_195227"
                     /isolate="SMAG_U2665"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.36444 N 132.31889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..591)
                     /locus_tag="VF958_12265"
     CDS             complement(<1..591)
                     /locus_tag="VF958_12265"
                     /inference="COORDINATES: protein motif:HMM:NF014754.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004371 - glycerone kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DAK2 domain-containing protein"
                     /protein_id="HEX9149929.1"
                     /translation="MISIGYLDGRRLSRAVIAGAHFVGQRAEPLNKINVFPVPDGDTG
                     TNLATTLQKIAAGIARVRERHVRRVSTAVADEALAGARGNSGAILAQFFSGFCEGLPD
                     RPRITTSDFGDAVVRAADSAYGAIARPVEGTILTVIRDWARYVANRAKDIRDFAVILP
                     DSLKEAKRSLENTPKQMAALRKAGVVDAGAQGFVYLL"
     gene            complement(588..863)
                     /locus_tag="VF958_12270"
     CDS             complement(588..863)
                     /locus_tag="VF958_12270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9149930.1"
                     /translation="MSTPFIRKCPFCGADMLRSQTHQSGYTHYFWQKPWKRSFFGLGA
                     ERVYPWACMGCGVVLFYLDRLPAVAEEFRSLRDVERAPGGQPAPLKP"
     gene            complement(991..2496)
                     /gene="gcvPB"
                     /locus_tag="VF958_12275"
     CDS             complement(991..2496)
                     /gene="gcvPB"
                     /locus_tag="VF958_12275"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014100585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006546 - glycine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase
                     subunit GcvPB"
                     /protein_id="HEX9149931.1"
                     /translation="MSVEVEEKAGVRTGNPLRPEPRHELLVFERSGEGKTGYSLPPLD
                     VPDVMAIPARLTRAEIAGETELTEVEVARHFTRLSKLNFSIDEALYPLGSCTMKHNPR
                     VNEEVARLAGFAASHPFAPEEVSQGALELAWRLEKILAELTGLSRVTLQPAAGAHGEL
                     AGILMVRAALEKEGNPRRTVLIPDSAHGTNPASARFAGYEVRELKSNERGTLDLRVLE
                     DAMTEDVAGLMLTVPNTLGIFENQILEIARIVHSKGGYLYCDGANFNSFVGIAKPGLM
                     GIDVMHMNLHKTFSTPHGGGGPGAGPVAVTERLVPFLPRPTVERRDDGSFFLDHDRPE
                     SIGRLRTFLGNFGMFVRALSYIWQYGNGIGEVARGAVLNANYVRAGLRGVYHLKYEAP
                     TMHEVVFSDKKQSEQGVHGGDIGKRLMDFGFHPPTISFPLIVHGALMIEPTETEGKAS
                     LDEFVAAMREIARECEEDPEIVRKAPHTTPVRRVDEVGAARNLILRWRPAK"
     gene            complement(2493..3836)
                     /gene="gcvPA"
                     /locus_tag="VF958_12280"
     CDS             complement(2493..3836)
                     /gene="gcvPA"
                     /locus_tag="VF958_12280"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015743289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006546 - glycine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase
                     subunit GcvPA"
                     /protein_id="HEX9149932.1"
                     /translation="MKFLPTSESDRQEMLRVIGASSVDDLFASIPSEVRKPADLPPPL
                     SEIEVRRRMGALAARTASARDTAFFLGAGLYHHWVSAIADQMLYRAEWLTSYTPYQPE
                     VSQGTLQSIFEYQTHICLMTGLDVSNASLYDGASALVEALLMAARLSRGRHKAVLSAA
                     IHPDYRETVRTYFTPLGLVAVEVPVGADGATDASALAAAVDADTFAVALQSPNFYGIV
                     EDWDAGSRAAHANGALSVAVVAEAVSMAILAPPGERGADIACGEAQSLGIPMHFGGPV
                     LGFLACRSEHQRQIPGRLVGETRDAEGRRAFCLTLATREQHIRRERATSNICTNQGLM
                     ALAANIHCSLAGKRGLREIATQSHAKAEYVKSKIAKVPGYRIPFAGPTFNEFVVEAPE
                     PAVPLLSRLARRGILAGVPLSRWDSRSENRFLVAVTEMNTREEMDRLVAALGGTA"
     gene            complement(3833..4225)
                     /gene="gcvH"
                     /locus_tag="VF958_12285"
     CDS             complement(3833..4225)
                     /gene="gcvH"
                     /locus_tag="VF958_12285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014099128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HEX9149933.1"
                     /translation="MNPQDLHYTQSHEWVRIDGDVGTVGITDYAQKELGEIVYLELPE
                     VGHMFNAEDEFGTVESVKAVSELFTPLSGEVIEINKGAVAEPGIVNDDPFGDGWLIKL
                     KLSTDEEVKKLLSAQQYEEFVKQEEKEK"
     gene            complement(4222..5337)
                     /gene="gcvT"
                     /locus_tag="VF958_12290"
     CDS             complement(4222..5337)
                     /gene="gcvT"
                     /locus_tag="VF958_12290"
                     /EC_number="2.1.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014099129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004047 - aminomethyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system aminomethyltransferase
                     GcvT"
                     /protein_id="HEX9149934.1"
                     /translation="MSTPVPAPAALRRTPLYDLHRELGAKLIDFGGWEMPVQYSGILE
                     EHRAVRERVGLFDVSHMGELEITGPDSLAALQWLTPNDVSRLVDGRCHYSAFLTERGT
                     FVDDLLVYRKAPDSYMLVVNASNTPKDFEWARSHARGNVRVEDASDRYALIAVQGPRA
                     VALVARLAADDPSNLAYYSFRQTSVLGAPALVSRTGYTGEDGFEICVASEDAARIYRA
                     LLEQGRSEGILPCGLGARDTLRLEAKMALYGNDIDETVTPWEADLGWIVKMKKGDFQG
                     RAALERQKADGVPRKLVGFEMVDRGIARHGYTARTAHGKGIVTSGTHSPTLGRPIGLA
                     LLPMDASAVGTEFEVDIRGRDAKTRVVATPFYKREAR"
     gene            5415..6359
                     /locus_tag="VF958_12295"
     CDS             5415..6359
                     /locus_tag="VF958_12295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012842650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1028 domain-containing protein"
                     /protein_id="HEX9149935.1"
                     /translation="MPVVLLLAFLQVVPRLSSAVPRPATPLVSTFSIVARDPANGDLG
                     VAVQSKFPNVRVAVPYAKAGVGAVATQSFSNSDFGTKGLELIELGATPQEVLAIIGRD
                     DPDLQDRQVGIVDAKGRSATFTGKKCFDWAGGRTGPDYAIQGNILVSEATVDAMEKAF
                     LGTKGPLADRLLAAIKAGAEAGGDRRGRQSAALVVVRPGASYDAKSDRYIDISVYDAK
                     DPIAEIFRLYELQKVHFERSDPKDVIPIGGADALYLQKLLAKKGFHKGRTDGVWDDAS
                     TKALADYMGWENYDTRIRQDGKIDRKILELIKGKEGPI"
     gene            complement(6398..6673)
                     /locus_tag="VF958_12300"
     CDS             complement(6398..6673)
                     /locus_tag="VF958_12300"
                     /inference="COORDINATES: protein motif:HMM:NF020967.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2007 domain-containing protein"
                     /protein_id="HEX9149936.1"
                     /translation="MKEEDEPEWVEVASVGNDDEATLIAGFLESQDISCEVEGPSGGT
                     PWPENLGAFGMSRVMVPPERADEAKRLLADRERMSLKSQDDDDTAVD"
     gene            complement(6677..7720)
                     /gene="selD"
                     /locus_tag="VF958_12305"
     CDS             complement(6677..7720)
                     /gene="selD"
                     /locus_tag="VF958_12305"
                     /EC_number="2.7.9.3"
                     /inference="COORDINATES: protein motif:HMM:NF002098.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004756 - selenide, water dikinase
                     activity [Evidence IEA];
                     GO_process: GO:0001887 - selenium compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="selenide, water dikinase SelD"
                     /protein_id="HEX9149937.1"
                     /translation="MGQFDLSALLAKLPVPADPNLLVGNASADDAAVYRIGEDRVMVQ
                     TIDFFTPVVDDPYLFGQIAAANALSDVYAMRARPVLALALVAFPTTVLPAEVLEDILR
                     GGADKVAEAGAVVGGGHSIDHDVPLYGLAVTGMSRERDLTRNAGARPGDALVLTKPLG
                     IGVTVCAGRADAIAAEGVTGLLRKRILSDDALEEASRVMAALNRPGVEAMEGFEIHAA
                     TDVTGYGLLGHAYEMMEASGTTAEFAVDAIPLLSQARRIAARGTAPGGSRANVRNLRP
                     RTTVGEGVTDDDILLLCDAQTSGGLLVALGEAEAERFAERCREKGADRTAVVGRVVAR
                     GESVLRVSRDSRR"
     gene            complement(7815..>7998)
                     /locus_tag="VF958_12310"
     CDS             complement(7815..>7998)
                     /locus_tag="VF958_12310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9149938.1"
                     /translation="VHDHPILAITAGALVGFYLGRSHGRQILSALVGVGLTAGASSAR
                     RMLGVAPEPRRAARAR"
BASE COUNT         1298 a         2751 c         2651 g         1298 t
ORIGIN      
        1 gaggaggtag acgaagccct gggcgccggc atcgacgacc ccggccttgc ggagagccgc
       61 catctgcttc ggcgtgttct cgagcgagcg cttcgcttcc ttcagagagt cgggcaggat
      121 gacggcgaaa tcgcggatgt ctttcgcgcg gttcgcgacg taccgcgccc agtcgcggat
      181 gacggtcagg atcgtccctt cgacggggcg cgcgatcgcg ccgtaggcgg agtcggcggc
      241 cctcaccacc gcgtctccga agtcgctcgt cgtgattcgc gggcgatccg gcagtccctc
      301 gcaaaaaccg ctgaagaact gggcgaggat cgccccggag ttgccccgtg cgcccgcgag
      361 ggcttcgtcc gcgaccgcgg ttgacacccg ccggacgtgt cgctcgcgga cccgggcgat
      421 tcccgcggcg atcttctgga gagtcgtcgc gaggttcgtg ccggtgtcgc cgtcgggcac
      481 cggaaagacg ttgatcttgt tgagcggctc ggcgcgctgc cccacgaagt gcgccccggc
      541 gatcaccgcg cgagacagcc ggcgaccgtc gaggtaaccg atcgagatca cggcttcagc
      601 ggagcgggct ggcctcccgg cgcccgttcc acgtcacgga gcgagcgaaa ctcttcggcg
      661 accgcaggaa ggcggtccag ataaaaaagg acgacgccac agcccatgca ggcccagggg
      721 tagacgcgtt cggcgccgag gccgaagaag gaccgcttcc acggcttctg ccagaagtag
      781 tgcgtgtagc cggactggtg cgtctgcgag cgcagcatgt ccgcgccgca gaaggggcat
      841 ttccggatga aaggggtgct catgaatcgc ggcggaagga tatcgcaggg aagagccggt
      901 tccggtcttc acgggccgca gcagtcgcca ccgaaccggg atggccttgc ctttctgcga
      961 aagcagattg gcaagtggag acctgccccc ctacttcgcc ggcctccacc tcagaatcag
     1021 gttcctcgca gcgccgacct cgtccacccg tcgcaccgga gtcgtgtgcg gcgctttccg
     1081 gacgatctcg ggatcttctt cgcactcgcg cgcgatctcg cgcatggcgg ccacgaactc
     1141 gtccagcgaa gcctttccct cggtctccgt cggctcgatc atgagagcac cgtggacgat
     1201 gagcggaaac gagatcgtcg gcgggtggaa tccaaagtcc atcaggcgct tcccgatgtc
     1261 tcccccgtgc acgccctgct cgctctgttt cttgtcggag aagaccacct cgtgcatcgt
     1321 gggcgcctcg tacttcaggt ggtacacccc tcggagcccg gcgcgcacgt agttggcatt
     1381 cagaaccgcc ccgcgtgcca cctcgccgat cccgttgccg tactgccaga tgtacgacaa
     1441 cgccctcacg aacatgccga agttgccgag gaaggtgcgg agccggccga tcgactcggg
     1501 ccgatcgtgg tcgaggaaga acgacccgtc gtcgcggcgc tcgaccgtcg gtctcggcag
     1561 gaagggaacg agcctctccg tcacggcgac cggccccgct ccgggccctc cgccgccgtg
     1621 cggcgtcgag aacgtcttgt gcaggttcat gtgcatcacg tcgatgccca tcagccccgg
     1681 cttggcgatc ccgacgaacg agttgaagtt cgccccgtcg cagtagagat agcccccctt
     1741 cgagtgaacg atgcgcgcga tctcgaggat ctggttctcg aagatcccga gcgtgttcgg
     1801 cacggtcagc atcagccccg ccacgtcttc cgtcatcgcg tcctcgagaa cgcgcaggtc
     1861 gagggtgcca cgctcattcg acttcagctc ccgcacctcg tatccggcga agcgagccga
     1921 ggcgggattc gtgccgtgcg ccgagtcggg gatcaggacg gtccgccgcg ggttgccctc
     1981 cttctccaga gccgcgcgga ccatgagaat tccggccagt tccccatggg cgccggcggc
     2041 cggctggagc gtcacgcggg agaggccggt cagctcggcc aggatctttt cgagccgcca
     2101 tgcgagctcg agcgccccct gggaaacctc ttcgggcgcg aaagggtgcg aggcggcgaa
     2161 gcctgcgagc cgcgcgacct cctcgttcac gcgcgggttg tgcttcatcg tgcaggagcc
     2221 cagcggatag agcgcctcgt cgatcgagaa gttgagtttc gagagtcgcg tgaagtgccg
     2281 cgcgacctcg acctcggtga gctccgtctc cccggcgatc tccgcgcggg tgaggcgggc
     2341 ggggatcgcc atcacatcgg ggacgtcgag gggcggaagc gaataccccg tcttcccctc
     2401 gcccgagcgc tcgaagacga ggagttcgtg gcggggctcg gggcgcagag ggttgccggt
     2461 ccggactccg gccttctctt cgacttccac gctcatgccg ttcctcccaa cgccgccacg
     2521 agccgatcca tctcttcgcg cgtgttcatc tcggtcaccg cgaccagaaa tcggttctcc
     2581 gaccgcgagt cccagcgcga cagcggaacc ccggcgagga tcccgcgccg cgcgagcctc
     2641 gagaggagcg gcaccgccgg ctccggggcc tcgaccacga actcgttgaa cgtcggaccc
     2701 gcgaacggta tgcgatagcc gggcaccttc gcgattttcg acttgacgta ctccgccttc
     2761 gcgtgggact gcgtcgcgat ctcccgcagc ccccgtttgc ccgcgaggga gcagtggatg
     2821 ttcgcggcga gcgccatcag cccctggttc gtgcagatgt tggaagttgc gcgctcccgg
     2881 cggatgtgct gctcacgagt cgcgagcgtc aggcagaatg cgcgccgccc ttctgcgtcc
     2941 cgcgtctccc cgacgagccg gcccgggatc tggcgctggt gctcggaacg gcaggcgaga
     3001 aaaccgagga ccgggccgcc gaagtgcatg ggtatgccga gcgactgcgc ctctccgcag
     3061 gcgatgtccg cgccgcgctc ccccggcggc gccaggatcg ccatcgagac cgcttcggcc
     3121 acgacggcca ccgacaacgc tccgttcgcg tgtgcggcgc gcgacccggc gtcccagtcc
     3181 tcgacgatcc cgtagaagtt cggggactgg agagcgaccg cgaaggtgtc ggcgtcgacg
     3241 gctgccgcga gcgccgatgc gtcggtcgca ccgtctgccc cgacgggaac ttcgaccgcc
     3301 acgaggccga ggggcgtgaa gtacgtgcgt acggtctccc ggtagtccgg gtggatcgcg
     3361 gcggacagaa ccgccttgtg gcgaccgcgc gaaagccggg ccgccatgag cagcgcttcg
     3421 acgagcgccg aagcgccgtc gtagagcgag gcgttcgaga cgtcgagccc cgtcatcaga
     3481 cagatgtgcg tctggtactc gaagatcgac tgcagggtcc cctgcgacac ctccggctgg
     3541 taaggcgtgt aggaggtgag ccactccgcc cgatagagca tctggtcggc gatcgcagac
     3601 acccagtggt ggtagagacc ggcgcccagg aagaacgccg tgtcgcgcgc gctggcggtc
     3661 cgagccgcga gtgcacccat ccggcggcgg acttcgatct cggagagcgg cggcggtagg
     3721 tccgcgggct tgcgcacctc ggacgggatc gaggcgaaga ggtcatcgac cgacgacgca
     3781 ccgatcaccc ggagcatctc ctggcggtcg gactccgacg tcggaaggaa tttcacttct
     3841 ccttctcctc ctgcttgacg aactcctcgt actgctgcgc cgagaggagc ttcttgacct
     3901 cttcgtccgt cgacagcttc agcttgatga gccatccgtc gccgaacgga tcgtcgttga
     3961 cgatgccggg ctcggcgacc gcccccttgt tgatctcgat gacctcgccg gagagcgggg
     4021 tgaagagctc cgagaccgcc ttcaccgatt cgacggttcc gaactcatcc tccgcgttga
     4081 acatgtgtcc gacttcgggg agctccaggt acacgatctc gcccagctct ttctgggcgt
     4141 agtcggtgat cccgaccgtg ccgacgtccc cgtcgatgcg gacccattcg tggctctggg
     4201 tgtagtgcag atcttgcggg ttcaccgagc ctccctcttg tagaacggtg tcgcgacgac
     4261 gcgcgtcttc gcgtcccgtc cgcggatgtc cacctcgaac tccgtcccca ccgcagaagc
     4321 gtccatcgga agcagggcga ggccgatcgg ccggccgagc gtcggagagt gcgttcccga
     4381 ggtcacgatg cctttaccgt gcgccgtgcg ggccgtgtag ccgtgacggg cgatgccgcg
     4441 gtcgaccatt tcgaaaccga cgagctttcg cgggacgccg tccgccttct ggcgttcgag
     4501 ggccgcgcgg ccctggaaat cgcccttctt catcttcacg atccaaccca gatcggcctc
     4561 ccagggcgtc acggtctcgt cgatgtcgtt cccgtacagg gccatcttgg cctcgagccg
     4621 gagcgtgtcg cgggctccca gaccgcacgg gaggattccc tcggagcgcc cttgttcgag
     4681 caatgcgcgg tagatcctcg cggcatcttc ggacgcgacg cagatctcga agccatcctc
     4741 tccggtatac cccgtgcgag aaacgagcgc aggagcaccg aggacggacg tctgccgaaa
     4801 cgaatagtac gcgagattcg agggatcatc cgccgcgaga cgggcgacga gcgccactgc
     4861 gcgaggcccc tggacggcga tcagggcgta acgatcgctc gcgtcctcga cccgcacatt
     4921 tccgcgcgcg tgcgagcgcg cccactcgaa atccttcgga gtattggacg cattgacgac
     4981 cagcatgtag gagtcgggtg ccttgcggta gacgagcagg tcgtccacga aggtcccgcg
     5041 ctccgtcagg aatgccgagt agtggcagcg accgtcgacc aatcgcgaga catcgttcgg
     5101 cgtgagccac tgcagggccg cgagtgagtc tggccccgtg atctcgagct cgcccatgtg
     5161 cgagacgtcg aagagcccga cgcgctcccg cacagcccga tgctcctcga ggatgccgct
     5221 gtactgcacg ggcatttccc agccgccgaa atcgatcagc ttcgcgccga gctcccggtg
     5281 caggtcgtag agcggcgtgc gccggagcgc cgcgggcgcg ggaaccgggg tggacatggg
     5341 cgggaagcta gcacaagacg ggagaaggga gatggtgatc tcgtagaatc cgcctgcctt
     5401 tcggaggttc tttcatgccc gtcgtattgc tgctggcgtt cctccaggtc gtcccccgtc
     5461 tctcctcggc cgtcccccgt cccgcgaccc cgctcgtttc caccttctcg atcgtcgccc
     5521 gagatccggc caatggcgat ctgggggtcg cggtccagtc gaagttcccc aacgtacgcg
     5581 tcgccgtgcc ctacgcgaag gcgggcgtcg gcgccgtagc cacccagtcg ttctcgaact
     5641 cggacttcgg gacgaagggt ctcgagctca tcgagctcgg agcgacgccg caggaggtcc
     5701 tggcgatcat cggacgcgac gacccggacc tccaggaccg ccaggtcggg atcgtggacg
     5761 cgaaggggcg ctccgcgacc ttcacgggga agaagtgctt cgactgggcg ggcggccgga
     5821 cgggccccga ctacgcgatc caggggaaca tcctggtctc cgaggcgacg gtcgacgcga
     5881 tggagaaagc ctttctcggg acgaaaggac cgctcgccga ccggctcctc gccgcgatca
     5941 aggcgggggc cgaggcggga ggagaccgcc gcgggcggca gtcggccgcg ctcgtcgtcg
     6001 tccgcccggg cgcttcctac gacgcgaaga gcgaccggta catcgacatc tccgtttacg
     6061 acgcgaagga tccgatcgcg gagatcttcc ggctctacga gctccagaag gtccacttcg
     6121 agaggagcga cccgaaggac gtcatcccga tcggcggtgc ggatgccctc tatctgcaga
     6181 agctcctcgc gaagaagggg ttccacaagg ggcgcaccga cggcgtgtgg gacgacgcct
     6241 cgacgaaggc gctcgcggac tacatggggt gggagaacta cgacacgcgg atccggcagg
     6301 acgggaagat cgaccggaag atcctggaat tgatcaaagg aaaagaaggg ccgatttagc
     6361 ggcaggactg acgtcgccga gagtccgagc gcggcggtca gtccaccgcg gtgtcgtcat
     6421 cgtcctgcga cttcaggctc atgcgctccc ggtccgccag cagccgcttc gcctcgtccg
     6481 ctcgttccgg ggggaccatc acgcggctca ttccgaacgc gcccaggttc tcgggccagg
     6541 gcgttccccc cgacggccct tccacttcgc atgaaatgtc ctgggactcc aggaatccgg
     6601 cgatcagcgt cgcctcgtcg tcgttcccga cggacgcgac ttcgacccat tccggctcgt
     6661 cttcttcttt cacgatctac ctcctcgaat ctcgggacac ccggagcacg gactcgccgc
     6721 gcgcgaccac acgcccgacg accgccgtcc ggtcggcgcc cttctcccga cagcgctcgg
     6781 cgaaccgctc cgcctccgcc tctccgagcg ccaccagaag cccgcccgac gtctgcgcgt
     6841 cacagagcag caggatgtcg tcgtcggtga ccccttcgcc gacggtcgtg cggggccgga
     6901 ggttgcgcac gttggcccgc gatcctccgg gtgccgtccc gcgtgccgcg atccgccgcg
     6961 cctgcgagag cagcggaatg gcgtcgaccg cgaactccgc ggtcgtcccc gacgcctcca
     7021 tcatctcgta agcgtggccg agcagtccat aaccggtcac gtccgtggcg gcgtggatct
     7081 cgaaaccttc catcgcctcg acccccggcc ggttgagcgc cgccatcacg cgggacgcct
     7141 cttccagcgc gtcgtccgag aggatccgtt tccggaggag tcccgtcacg ccttccgccg
     7201 cgatcgcgtc ggcgcgtccg gcgcaaaccg tgacgccgat cccgaggggc ttcgtcagca
     7261 cgagcgcgtc tcccggccgc gcgcccgcgt tgcgcgtcag gtctctctcc cgcgacatcc
     7321 ccgtgaccgc gaggccgtag agcggcacgt cgtgatcgat cgaatggccg ccgcccacga
     7381 ccgcaccggc ctctgcgact ttgtcggcgc cccctctcag gatgtcctcg agcacttcgg
     7441 ccgggagaac cgtcgtggga aacgccacga gggcgagcgc gagaacgggc cgcgcgcgca
     7501 tggcgtacac gtcggagagc gcgttcgccg ccgcgatctg gccgaagagg taaggatcgt
     7561 cgaccacggg cgtgaagaag tcgatcgtct ggaccatcac ccgatcctcc ccgatgcggt
     7621 acaccgcggc gtcgtcggcg gacgcgttgc cgaccagcag attcggatcc gccggaaccg
     7681 gcagcttcgc gagaagcgcg gagaggtcga actgaccgat cttgcaggct cagcccgcgc
     7741 tcgacgcgaa cgtcgagagc gcagggtgac ccgctttgcg gtacgtcacg accggattat
     7801 agggagatag cggactagcg ggcccgcgcc gcccgtcgcg gctccggcgc tactccgagc
     7861 atccggcgcg cgctcgaggc accggcggtg agtcccacgc ccaccagagc cgagaggatc
     7921 tggcgcccgt ggctccgccc gagatagaag cccacgagcg ctcctgccgt gatcgccagg
     7981 atcggatgat catgcacc
//
LOCUS       DASWAV010000592         6056 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U2665
            k141_715274, whole genome shotgun sequence.
ACCESSION   DASWAV010000592 DASWAV010000000
VERSION     DASWAV010000592.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960316
            Sequence Read Archive: SRR10260007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 6056)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6056)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:47:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,929
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6056
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_715274"
                     /isolate="SMAG_U2665"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.36444 N 132.31889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            120..1751
                     /locus_tag="VF958_12315"
     CDS             120..1751
                     /locus_tag="VF958_12315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013177876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HEX9149939.1"
                     /translation="MTKKPGLLTGAVVGLFLTAPLVAIFYAGWKLAGLPFVPFQVFDW
                     TARVLPGRVITFGIDTMVKVIRALNIADTSTAAKAAEQSMAIAVFLVIGVLAGALLFA
                     FLRLLRSRLSVFPGLVAGALLGAGSVALARSLGRVEAVPRSIGEAWTIAAWLGWGMAL
                     GLANRRLSRRRIALPGEAARDAWDDRVERVDRRRFIVKLGAATATITVAGAVVGKFVG
                     KSLRPAEVATGKRWSVDHPLPNAGAAVKPAPGTRPEYTPLEDHYRIDITTIPPVVDGA
                     RWRLKVGGLVERPLELPLDRIRAYEPLHQFVTLACISNPVAGTLIGTTRWTGVSFQRL
                     LPDLRLKSNATHLKITSADRFYEVVALETIRSDPRVMLAYEWDGVPLTTPHGFPLRIY
                     IPDHYGMKQPKWILEIEAIDHWEPGYWVKRGWDQTAQMKATSVIDTVSVDMMLSEADR
                     NTRIPVGGIAHAGARGISKVEVRTDDGPWHEAQLRTPLSGTTWVVWRYDWPFQKGQHR
                     LTVRCFDGSGALQNAELAPPHPSGASGYHEKAVML"
     gene            1806..2015
                     /locus_tag="VF958_12320"
     CDS             1806..2015
                     /locus_tag="VF958_12320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006020185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6496 domain-containing protein"
                     /protein_id="HEX9149940.1"
                     /translation="MARYGKKASEKVEKAMHERKRGTLKSGRSGKKVTSRKQAIAIGL
                     SEARREGGKVPRKKSSSKRSSSKKK"
     gene            2120..3214
                     /locus_tag="VF958_12325"
     CDS             2120..3214
                     /locus_tag="VF958_12325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9149941.1"
                     /translation="MIRVARLEAAGLAVAVLALAATVRANAGSQDPLAAEIERWSAFA
                     RDSRETHAMWAPVKQATEPVLARARAALAAGRRFLALQQLGAARVNLAAFLYLAGLPA
                     SERKDLSRLESEWARDGRALKADLAKTPPSAFADVQPAAIRAVAEAALPQVRAFYDAS
                     LDYGHSTDAEAGLFYLGAARSQREFVSFCRRLSTPSPLRAPGLRSLAPELDALEGELL
                     ARYRPPAAIERHGDFIAASSVLKEARELDAAGLRYGALLRYLLAAQRSAQLRPDPPAF
                     DAAAIAARMRELDRLLSAKGIDHSIGRLFLESAQAEAASVAPNSPSPVAAAIVSDLIP
                     RYFAALEPRRAEPPKPAPLVTVTLVRWPYT"
     gene            3236..3979
                     /locus_tag="VF958_12330"
     CDS             3236..3979
                     /locus_tag="VF958_12330"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HEX9149942.1"
                     /translation="MLAQSVVREFAGKARFVSENYGGSALAKRFGVTRYPAIFVNDVL
                     VATPKDFGFYGKGEGAGDGRYTPLKSADAHERFRSDLSRSIRLVLEGRKDAARAAAPS
                     PTGGEVAAFPDVVLTDLEGRPISRRHLAGRAVLVEFWATWCPPCRGTLGWLGEVKKRY
                     GERLTVIAVAIESDESAVRKVALERELPLRWTMGTPDVARAFGDVSGVPTLLLFDGAG
                     KAAAAFYGAPPDLHSRAETKLAAVIQPLP"
     gene            4026..5465
                     /locus_tag="VF958_12335"
     CDS             4026..5465
                     /locus_tag="VF958_12335"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HEX9149943.1"
                     /translation="MLIKSLVAAIALLLSGAAERTPADAVGRLGEIIPKAMREAEVPG
                     VSVALIRDGRVAWSRAFGVKNVETRAAMDEKTVFESASLSKPVFAYAVMKLVDAGKLA
                     LDVPLVKYMPVPAVLGDERAGRITARMVLDHTTGFQNEAIGKPLAIHFTPGTRFSYSG
                     AGYAYLQTIVERVTGEPLEAFMKKAVFGPLGMTSSSFVWQDGYASRRATGYKMDGTAG
                     RDRRPSEGKASSSLFTTAPDYARFVLAAMNGTGLTKESARRMLETQVRVEESCFLCVD
                     RKASKLSPALSWGLGWGLERTEDGPAFWQWGDYNGEVQSFAIGIPRTKSGIVVLTNSG
                     SGLSIVPAIVTAALRGDHPVFAWLPYEPYDSPGLLFYREILERGGPAIRRYRETRKTG
                     NGVRLTETQMNDLGYRLLAKEKNEGALEIFKINAEDFPSSSNVHDSLGQGYLAVGDVD
                     AAIKSYRKSLELDPSNENARNVLKQLKAE"
     gene            complement(5478..>6056)
                     /locus_tag="VF958_12340"
     CDS             complement(5478..>6056)
                     /locus_tag="VF958_12340"
                     /inference="COORDINATES: protein motif:HMM:NF022588.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2911 domain-containing protein"
                     /protein_id="HEX9149944.1"
                     /translation="TYSLHTIPGEKEWTIIFNKEANQWGSYSYEAAKDALRVKVQPVP
                     GAPQEWMQFRFEDPSINSARVVLAWENLQVPFTIRNATDTNTKVVAMLRESVAKARPD
                     EWQAYYRAGNYLVQNKISMDEAGAWLDKAIAISPTPQTHYAKARYFDAAGKSREAIAE
                     LDRALAAAKADTPSTLVDEIRNVKRTWSEKKS"
BASE COUNT          940 a         2046 c         2052 g         1018 t
ORIGIN      
        1 acacctcgaa tcggctggtt ttcgcctcga agaagtgatc gagcgggatc catatccgga
       61 ggtcgaatat ccgagcaggc gggcgtacgt cttcgccaga aagagatagc gttatcgcca
      121 tgaccaagaa gccgggtctc ctgacaggag cagtggtcgg tctctttctg actgcgccgc
      181 tcgtagcgat cttctacgcc ggatggaagc tcgccggcct gccgttcgtt cccttccagg
      241 tcttcgactg gaccgcgcgc gtgctgccgg gccgcgtcat caccttcggc atcgacacga
      301 tggtgaaggt gatccgcgcc ctcaacatcg ccgatacctc gaccgcggcc aaggcggcgg
      361 aacagagcat ggcgatcgcg gtgttcctcg tcatcggcgt cctggccggc gcgcttctct
      421 tcgcgttcct ccgcttgctg cggtcgcggc tgtcggtctt tccggggctc gtcgcgggcg
      481 cgctcctggg cgccggatcg gtcgccctgg cgcgaagcct cgggcgggtc gaggcggtcc
      541 cgcgatcgat cggagaagcc tggacgatcg cggcgtggct cggctggggg atggcgctcg
      601 gcctcgcgaa ccggcgtctc tcgcgacggc gcatcgcgct ccccggcgag gccgcgcgcg
      661 acgcctggga cgatcgggtc gagcgcgtcg atcgtcgccg cttcatcgtg aagctcggcg
      721 ccgcgacggc gacgatcacc gtcgccgggg ctgtcgtcgg aaagtttgtc gggaagagtc
      781 tgcgccccgc cgaggtggcg accggaaagc gctggtccgt cgaccatccc cttccgaacg
      841 ccggcgccgc cgtaaagccc gcgcccggca cgcgcccgga gtacaccccg ctcgaagacc
      901 attaccgcat tgacatcacg acgattccgc cggtcgtgga cggtgcgagg tggcggctga
      961 aggtcggcgg cctcgtggag cggcctctcg agctgccgct cgaccggatc cgcgcttacg
     1021 agccgctcca ccagttcgtc actctcgcgt gcatctccaa tcccgtcgcg ggcacgttga
     1081 tcggcacgac gcgctggacg ggtgtcagct tccagcgcct tctcccggac cttcggctga
     1141 aatcgaacgc gacgcacttg aagatcacct cggcggaccg tttctacgag gtcgtggcgc
     1201 tcgagacgat ccggagcgac ccccgcgtga tgctcgccta cgaatgggac ggggtcccgc
     1261 tgacgacgcc gcacgggttt cccctgcgga tctacatccc cgaccactac gggatgaagc
     1321 aacccaagtg gatcctcgag atcgaggcga tcgatcactg ggagccgggc tactgggtca
     1381 agcgcggctg ggaccagacc gctcagatga aagcgacgtc ggtcatcgac acggtgtcgg
     1441 tcgacatgat gctctcggag gccgaccgga acacccggat tcccgtgggc ggtatcgctc
     1501 acgcgggcgc gcgcggcatc tccaaggtcg aggtgcgaac ggacgacggg ccgtggcacg
     1561 aggcgcagct gcgcacgccg ctgtccggca cgacgtgggt cgtctggcgc tacgactggc
     1621 cgttccagaa ggggcagcat cggctgaccg tgcgctgctt cgacggcagc ggtgcgttgc
     1681 agaacgccga gctcgcgccg cctcacccct ccggcgcatc ggggtaccac gaaaaggccg
     1741 tgatgctctg acgtcgcggc acgtttggca tcgttcctgc gagcgccgga atcggaggtc
     1801 gaacgatggc ccgatacgga aagaaggcgt cagaaaaagt cgagaaagcg atgcacgagc
     1861 gcaagcgcgg cacgttgaag agcgggcggt ccggcaagaa ggtaacgagc cgcaagcagg
     1921 cgatcgccat cggcctgtcg gaagcgcgga gagagggggg caaggtgccc cgcaagaagt
     1981 cctcctcgaa gaggtcctcc tcgaagaaga agtagcgccg cttcaggcgt cccgcccggg
     2041 cgagtcgtcc tcctgccacc ggctttcgtc atccgcggat gggcggcgcg ccgtctcggc
     2101 gtgcgatgct cctcggcgaa tgatccgcgt tgcccgactc gaagcggcgg ggctcgccgt
     2161 ggctgtgctg gcgctcgccg ccacggttcg cgcgaacgcc ggctcgcagg atccgctcgc
     2221 ggccgagatc gagcggtggt ccgcgttcgc ccgcgactca cgggagaccc acgcgatgtg
     2281 ggcgccggtg aagcaggcga cggagcccgt cctcgcacgg gcgcgggcgg cgctcgccgc
     2341 cggacggcgc tttctcgctc tccagcaact gggcgcggcg agggtcaacc tcgccgcctt
     2401 cctctacctt gcgggccttc cggcctccga gcgaaaagac ctttcgcgtc tcgagtcgga
     2461 gtgggcccgc gacggacgag ccctgaaggc cgacctggcg aagacgccgc cgtccgcctt
     2521 cgcggacgtt cagccggccg cgatccgcgc cgtcgccgag gccgctctgc cccaggttcg
     2581 cgccttctac gacgcgagcc tcgactacgg ccacagcacg gatgcagagg cgggactgtt
     2641 ctacctcggc gcggcgcgtt cccagcgcga gttcgtgtcg ttctgccgcc ggctctcgac
     2701 tccgtcgccg ctccgcgcgc cggggcttcg atcgcttgcc ccggagctcg acgcgctcga
     2761 aggggagctt ctcgcccgtt atcgcccgcc ggccgcgatc gagcgccacg gcgatttcat
     2821 cgcggcgagc tcggtcttga aagaggcccg cgagctcgac gcggcggggc ttcgctacgg
     2881 agcgcttctg cgctatctcc tggccgcgca gcggagcgcg cagctccggc cggatcctcc
     2941 ggcgttcgac gcggccgcaa tcgccgccag gatgcgcgag ctcgaccggc ttctctccgc
     3001 gaaagggatc gatcacagca tcggccggct cttcctcgag agcgcacagg ccgaggccgc
     3061 gagcgttgcg ccgaattccc cgtcgccggt ggccgcggcg atcgtctccg acttgatccc
     3121 tcgctacttt gcggcgctcg agccccgccg agcggagccc ccgaagcccg ccccgctcgt
     3181 cacggtgaca ctcgtccgct ggccctacac ctgaaatctc tccgacccgg caagtttgct
     3241 ggcccagagc gtcgtgcggg agttcgcggg gaaggcgcgc tttgtgtcgg agaactacgg
     3301 cggctccgcc ctcgcgaaac gcttcggcgt gacgcggtat cccgcgatct tcgtcaacga
     3361 cgtgctcgtc gcgactccca aggatttcgg cttctacgga aagggtgagg gcgcgggcga
     3421 cggacgctat acgccgctga agagcgccga cgcgcacgag cggtttcgct ctgatctgtc
     3481 gcgaagcatc cgtctcgttc tcgagggccg caaggacgcg gcgcgcgccg ccgcgccctc
     3541 gccgaccgga ggcgaggtcg ccgcgtttcc cgacgtcgtc ctcacagacc tggagggccg
     3601 tccgatctcc cggcgccacc tcgcggggcg cgcggtcctc gtcgaattct gggcgacgtg
     3661 gtgtccgccg tgccgcggca cactcgggtg gctcggagag gtcaaaaagc ggtacggaga
     3721 gaggctcacc gtaatcgctg tcgcgatcga gtccgacgag tcggccgtcc gcaaggtcgc
     3781 gttggagagg gagctgccgc ttcgctggac gatggggacg cccgacgtcg cccgcgcgtt
     3841 cggggacgtg agcggggtcc cgacgctcct gctgttcgac ggggcgggga aggcggcggc
     3901 cgcgttttat ggcgcgccgc cggatctgca ctcgcgcgcc gagacgaagc tcgcggctgt
     3961 catccagccg cttccctgat ctgcgctcca ccagccgttc tggcatcagt cgctccggac
     4021 ggggcgtgct gatcaaatct ctcgtcgcgg cgatcgctct cctcctttcg ggagcggcgg
     4081 agcgcactcc ggcggacgcc gtcgggcggc tcggcgaaat catcccgaag gcgatgcggg
     4141 aagccgaggt gccgggcgtc tccgtcgcgc tgattcggga cggacgcgtc gcgtggagcc
     4201 gcgccttcgg cgtgaagaac gtcgagaccc gggcggcgat ggacgagaag acggtcttcg
     4261 agtcggcgtc gctcagcaag ccggtcttcg cgtacgccgt gatgaagctc gtcgatgccg
     4321 gcaaactggc gctcgacgtt ccgctcgtga agtacatgcc cgttccggcc gtgctcggag
     4381 acgagcgtgc cggccggatc actgcgagga tggtgctcga ccacacgacc gggtttcaga
     4441 acgaagcgat cggcaagcct ctcgcgatcc atttcacgcc cggcaccagg ttcagctact
     4501 cgggcgccgg ctacgcgtat ctgcagacga tcgtcgagcg cgtcaccggc gagccgctcg
     4561 aagcattcat gaagaaagcc gtcttcggcc cgctcgggat gacgagcagc agcttcgtct
     4621 ggcaggacgg ctacgcctcc cgccgcgcga ccggatacaa gatggatgga accgccggaa
     4681 gggaccgcag gccgtccgag gggaaggcgt cgtcgagtct cttcacgacg gctcccgact
     4741 atgcccggtt cgtcctcgcg gcgatgaacg gaacggggct cacgaaggag agcgcgcggc
     4801 ggatgctcga gacgcaggtc cgggtcgagg agagctgttt cctgtgcgtc gaccgcaagg
     4861 cctcgaagct ttctcccgct ctctcctggg gcctcggctg gggactcgag cgcacggagg
     4921 atggcccggc gttctggcaa tggggcgact acaacgggga agtccagagc ttcgccatcg
     4981 ggatccctcg gaccaagtcc ggaatcgtgg tcctgaccaa cagcggcagc ggtctctcga
     5041 tcgttcccgc gatcgttacc gcggcgcttc gaggcgacca tccggtcttc gcgtggctgc
     5101 cgtacgagcc gtatgactca ccgggtcttc tcttctaccg cgagatcctc gagcggggcg
     5161 ggccggcgat ccggcggtat cgcgagaccc ggaagaccgg caacggcgtt cggctgaccg
     5221 aaacccagat gaacgacctc ggctaccgcc tcctcgcgaa ggagaagaac gagggagcgc
     5281 tcgaaatctt caagatcaac gcggaagact tcccgagctc atcgaacgtc cacgacagcc
     5341 tcggccaggg atacctggcc gtcggtgacg tcgacgcggc gatcaagagc taccgcaagt
     5401 ctctcgagct cgacccttcg aacgaaaacg cccgcaacgt gctgaagcag ctgaaggcgg
     5461 agtaaaaccg gatctcgtca gctcttcttc tcgctccagg tcctcttcac gttccggatc
     5521 tcgtcgacga gcgtggaggg ggtgtctgcc ttcgcggccg caagtgccct gtcgagctcc
     5581 gcgattgcct cccgcgactt cccggcggcg tcgaagtacc gtgccttcgc gtagtgggtc
     5641 tgaggcgtcg ggctgatcgc gatggccttg tccagccacg cgccggcttc gtccatcgaa
     5701 atcttgttct ggaccaggta gtttccggcg cggtaatacg cctgccactc gtccggcctg
     5761 gccttcgcga cgctttcgcg cagcatggcg acgactttcg tgttcgtgtc ggtcgcgttc
     5821 cggatcgtga acggtacctg caggttttcc cacgccagga cgactcgcgc cgagttgatg
     5881 gaaggatctt cgaagcggaa ctgcatccac tcctgcgggg cgcccggaac cggctggacc
     5941 ttcacccgca aggcgtcctt cgcggcttcg tagctgtaag aaccccactg gttcgcttcc
     6001 ttgttgaaga tgatcgtcca ctccttctcg cccggaatgg tgtggagcga ataggt
//
LOCUS       DASWAV010000593         2101 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U2665
            k141_274182, whole genome shotgun sequence.
ACCESSION   DASWAV010000593 DASWAV010000000
VERSION     DASWAV010000593.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960316
            Sequence Read Archive: SRR10260007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2101)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2101)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:47:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,929
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2101
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_274182"
                     /isolate="SMAG_U2665"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.36444 N 132.31889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1757)
                     /locus_tag="VF958_12345"
     CDS             complement(<1..1757)
                     /locus_tag="VF958_12345"
                     /inference="COORDINATES: protein motif:HMM:NF025006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HEX9149945.1"
                     /translation="MRFRRLLIALAIVCLATPLAFAQTTGSLAGVVRNPEGGALPGVT
                     VTISGEKLPLGRSMTTLSDGAFQFIGLIPGEYRLKAELPGLGMFEQNVIVGLGEETQV
                     RAVLRATATAAVEVSAAVPLVDTKASDISAVTTSEAIRKLPIGGSYSATFALAAGVPE
                     NTTTAVSAPNAGGGRQDNTFLYDGVNVTNPFFGDLFQDFTGIDVQEVNITRGGVSPEY
                     GRTGGFLVNAVTKSGSNNFHGSGSLEYSPVAWTSDSIDPTIQRSKFERFRPGVQIGGP
                     LWRDHLFFYGSANWYRVTEKDRANQFGTSATNTTPNDLSDAKTDTDEYFGKLTANPTS
                     SILIDGSYRWRSIDSTNSGVTSRAAASTGSNGKTEDRVGVVTGLWTITPTWTLEAKYN
                     HNEDNNSTDPVTDLGYQPAFNPTAPYLVGLFTTSITGGVNFVFPPAGDVNQVMGGTDL
                     AINDQDFIRDEYRLQTSLLANFLGGTHDIRFGATYSHNEEDLLRVANGWGSIIASTSS
                     LCGPASARPCYRARFNPRQPAQISEAETWGLFLQDQITWNRLTLNLGVLANRDEFIPN
                     DGLKFTILRGDFTIPNANIP"
     gene            complement(1864..>2101)
                     /locus_tag="VF958_12350"
     CDS             complement(1864..>2101)
                     /locus_tag="VF958_12350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007223007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HEX9149946.1"
                     /translation="DVVGPICETSDFFLRDSVMDLPAEGDLLAIRDVGAYGFSMSSNY
                     NFRGRPPEVWVEGGSYRIIRRRETFEDMVREERE"
BASE COUNT          363 a          641 c          709 g          388 t
ORIGIN      
        1 gggatgtttg cgttcggtat cgtgaaatcg ccacgcagga tcgtgaactt gagtccgtcg
       61 ttggggatga actcgtcacg gttggcgagc acgccgaggt tcagggtcag ccggttccag
      121 gtgatctggt cctgcaggaa gagcccccac gtctcggcct cggagatctg ggcgggctga
      181 cgggggttga atctcgcccg gtagcagggc cgcgcggaag ccggaccgca gagcgatgag
      241 gtcgacgcga tgatcgagcc ccagccgttg gcgacccgga gaagatcttc ctcattgtga
      301 ctgtacgtgg cgccgaagcg gatgtcgtgc gtcccgccga ggaaattcgc cagcagcgag
      361 gtctgcagcc ggtactcgtc gcggatgaag tcctggtcgt tgatggcgag gtccgtcccc
      421 cccatcacct ggttcacgtc gcccgccggc ggaaacacga aattgacgcc gcccgtgatc
      481 gaggtcgtaa agaggccgac gagataggga gcggtcggat tgaacgccgg ctgatagccg
      541 aggtcggtga ccggatcggt cgagttgttg tcctcgttgt ggttgtactt ggcctcgagg
      601 gtccaggtcg gggtgatcgt ccagagaccc gtgacgacgc cgacgcggtc ctcggtcttg
      661 ccgttggagc ccgtgctcgc ggccgcgcgc gaagtgacgc cggagttcgt gctgtcgatg
      721 ctgcgccagc ggtaggagcc gtcgatcagg atgctcgaag tcgggttcgc ggtgagcttc
      781 ccgaagtatt cgtcggtgtc ggtcttggcg tccgacaggt cgttcggggt cgtgttcgtg
      841 gctgacgtgc cgaactggtt tgcgcgatcc ttctcggtca cccggtacca gttcgccgag
      901 ccgtagaaga agagatggtc gcgccagagc ggtccgccga tctgaacgcc gggccggaag
      961 cgctcgaact tgctccgctg gatcgtggga tcgatcgagt ccgatgtcca tgccaccggc
     1021 gaatactcga gcgacccgct cccgtggaag ttgttcgagc ccgacttcgt gaccgcgttg
     1081 acgaggaagc caccggtcct cccgtactcc ggcgaaactc cgccgcgcgt gatgttgacc
     1141 tcctggacgt cgatccccgt gaaatcctgg aagaggtcgc cgaagaacgg gttcgtcacg
     1201 ttgacgccgt cgtacaggaa cgtgttgtcc tggcggccgc ctccggcatt cggcgcgctc
     1261 acggcggtcg tggtgttttc cgggacgccg gccgcgagag cgaaggtcgc tgagtaactg
     1321 ccgccgatcg ggagcttccg gatcgcctcg ctcgtcgtga cggccgagat atcggaggcc
     1381 ttggtgtcca cgagcggaac cgcggcactc acctcgaccg cggcggttgc ggtcgctctg
     1441 aggactgctc ggacctgagt ctcctcgccg agtccgacga tgacgttctg ttcgaacatg
     1501 ccgagacccg gcagctcggc cttcaaccgg tattccccgg gtatgagccc aatgaactgg
     1561 aacgcgccgt cggagagcgt cgtcatcgag cggcccaacg gcaacttctc accggagatc
     1621 gtgacggtca ctcccgggag agccccgcct tcggggttcc tcacgacgcc cgcgagtgag
     1681 ccggtggtct gcgcgaacgc gagcggtgtg gcgagacaga cgattgcaag agcgataagt
     1741 aaccggcgga aacgcatcga gcagtcctcc ttcaggatgc gcggacgaac ggccgcgcga
     1801 aaagtacttc cgcatgcgaa cggcggacga aactcaaaaa catgggctga acttcgcgcg
     1861 attttactcc cgctcctcac gaaccatgtc ctcgaacgtc tcccggcggc ggatgatccg
     1921 atacgagcct ccctcgaccc agacctccgg cggtctcccc cgaaagttgt agttcgatga
     1981 catggagaag ccgtaggcgc cgacgtcgcg gatcgcgagc aggtcgccct ccgcggggag
     2041 atccatgacg ctgtctctca ggaagaagtc gctcgtctcg cagatggggc cgacgacatc
     2101 c
//
LOCUS       DASWAV010000594         1713 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U2665
            k141_635821, whole genome shotgun sequence.
ACCESSION   DASWAV010000594 DASWAV010000000
VERSION     DASWAV010000594.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960316
            Sequence Read Archive: SRR10260007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 1713)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1713)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:47:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,929
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1713
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_635821"
                     /isolate="SMAG_U2665"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.36444 N 132.31889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..503
                     /locus_tag="VF958_12355"
     CDS             <1..503
                     /locus_tag="VF958_12355"
                     /inference="COORDINATES: protein motif:HMM:NF013955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IPT/TIG domain-containing protein"
                     /protein_id="HEX9149947.1"
                     /translation="YGDYCTGKIWGATRTVTGSWSTGLFSPRAAGLTTFGEDAAGEIY
                     LATESGRLARVVDANPAVAPTVVSVEPVSGSTRGGESVIVTGSGFASGVTVLFGGVLA
                     TSVTILDSNGTQLRLTTPAHAAGIVDVVASNPDGRSGTFTSGYGYAAPSRVALPPRIP
                     RTVTRP"
     gene            complement(710..>1713)
                     /locus_tag="VF958_12360"
     CDS             complement(710..>1713)
                     /locus_tag="VF958_12360"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HEX9149948.1"
                     /translation="GGGFRVMTTQIVASKLNGDIAMAMVETVTLAAVALIGLFLLQWS
                     ERGRGVTGGGRGIPPARRRLERSRWVAAALGWLLALVLLLPHATLVLVSFVPRGTWTV
                     EILPPVLDLSNYASLLSEPERLRPLVNSLWMAAAATAAALALGFAAARLAVRAKSRLG
                     GLLETLLTIPWALPGTVFAVALSAAFSVHAPWIGRFVLVGTAAILPLAYLVRSLPLTG
                     RAAFAGFRQLDPALDEAAASLGASRWRRLLRVDLPLLAPALAAGASLAFITALGDFVV
                     SIVLYTFETRPISIEILSSLRLNETGVAAAYGVVLATLSAVVFFVWGRRGDAVRPPG"
BASE COUNT          308 a          584 c          600 g          221 t
ORIGIN      
        1 tttacggcga ctactgcacc ggcaagatct ggggagcgac gcgcaccgtc acgggatcgt
       61 ggtcgacagg gcttttctct ccgcgcgcgg cgggtctgac gaccttcggc gaagacgcgg
      121 cgggcgagat ctacctcgcc acggagagcg ggcggctcgc gcgtgtcgtc gacgccaatc
      181 cggcggttgc gccgacggtc gtctccgtcg aacccgtctc cgggtcgacg cggggcggcg
      241 agtcggtcat cgtcacggga agcggcttcg cttccggcgt gacggtcctg ttcggcggcg
      301 tgcttgcgac ttccgtcacg attctcgatt cgaacggcac gcagcttcgc ctgacgacac
      361 ccgcgcacgc cgccggaatc gtcgacgtcg tcgcctcgaa cccggacggc cggagcggga
      421 cgttcacgtc gggatacggg tacgccgctc cgtcgcgcgt tgcgttgccg ccccggattc
      481 cgcgcaccgt gactcgaccc tgagaactcc ttcagccgaa aggtgaatgt gatcgtcatg
      541 tagacagcga tgggtttgcc actctgacgc gccgggttcg caggaaacac ctccatcggg
      601 ttaccagggc gccgcgagcg ctgactgatg tggcaagtgg agacctgacc ctcaatgtgg
      661 caagtggaga cctgaccctc aatgtggcaa gtggagacct gaccccatct cagccgggcg
      721 ggcgcacggc gtcgccgcgg cgcccccaca cgaaaaagac caccgccgaa agcgtcgcca
      781 gcacgacgcc gtacgcggcg gcgacgccgg tctcgttcaa gcgcagcgag gagaggatct
      841 cgatcgagat cggccgcgtc tcgaacgtgt agagcacgat cgacacgacg aagtctccca
      901 aggcggtgat gaaggcgagg ctcgcgcccg ccgcgagcgc cggagcgagc agcggcaggt
      961 cgactcgcag gagcctcctc cagcgggagg cgccgagcga cgcggccgcc tcgtcgagcg
     1021 cgggatcgag ctgtcggaac ccggcgaagg ccgcgcggcc ggtcagcggg aggctccgaa
     1081 cgaggtacgc gagcgggagg atcgccgcag taccgacgag cacgaaccgg ccgatccacg
     1141 gcgcgtgaac ggaaaacgcc gcggacagcg ccaccgcgaa gaccgtgccg ggcagcgccc
     1201 acgggatcgt gaggagggtt tcgagaagcc cgccgagacg cgacttcgct ctgaccgcca
     1261 gtcgcgccgc cgcgaacccg agagcgagcg ccgcggccgt cgccgccgcc gccatccaga
     1321 gcgagttcac gagcgggcga agcctctcgg gctcggagag gagcgatgcg tagttcgaga
     1381 ggtcgagcac cggaggcagg atttcgaccg tccaggtccc ccgtggcacg aaggagacga
     1441 ggacgagcgt cgcatgggga aggaggagca ccagagccag cagccatccg agcgcggccg
     1501 cgacccaccg cgaccgctcg agacgacgtc gagcgggcgg gatcccgcgt ccgccacccg
     1561 tgacgccgcg accccgttcg ctccactgca gcaggaaaag accgatgagc gcgaccgccg
     1621 cgagcgtgac ggtctcgacc atcgccatcg cgatgtcgcc gttcagcttc gacgcgacga
     1681 tctgcgtggt catcacccga aagccaccgc cga
//
LOCUS       DASWAV010000595         2029 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U2665
            k141_274445, whole genome shotgun sequence.
ACCESSION   DASWAV010000595 DASWAV010000000
VERSION     DASWAV010000595.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960316
            Sequence Read Archive: SRR10260007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2029)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2029)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:47:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,929
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2029
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_274445"
                     /isolate="SMAG_U2665"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.36444 N 132.31889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1367)
                     /locus_tag="VF958_12365"
     CDS             complement(<1..1367)
                     /locus_tag="VF958_12365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9149949.1"
                     /translation="MRPVTSDLDAEFARAADLLEAGDRTTAATVLAEIARKAGQRAWH
                     ARISLLLAEDDERRKDFASAEKNLRAAEAAAIGLEPYRRAWLGRVLEAAGRREAAIKE
                     WRGALESAEPFARRTAVARDLARALEVSGRVRVAIAVLNGVAASAPGSNFAEIGVERI
                     RLALTIGDVQTVTSAAHALLIRAPALDAVSSTPESVREALKREEKRLSAADRARRGRA
                     LVAAGDSRRGQRLLLERPGAWAPEDRPSNHLALARALTALGRSGAADAEAARVPRRTP
                     QWFEATLFRADLLLERLRKKADGALLAKTPRIVPVRRMLESVAVASAPAPTRAAAWER
                     LVRLAAEAEDFDGALALARQLTSQTRGSVRGFEPIWELAWKNWLSRDFPGARRRFEAL
                     AATYNDIWRDRRLAYWRARCLEKENRKDEAGAIYARLAAGDPPDLYALFSRRRTHGTT
                     GRAPAP"
     gene            complement(1476..>2029)
                     /locus_tag="VF958_12370"
     CDS             complement(1476..>2029)
                     /locus_tag="VF958_12370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9149950.1"
                     /translation="DAVALLTWIAGLAVDRIAVRKLAPSPALRAFRGAEEAAEADVSP
                     SFASTPSVERPAAPLPSDESTATPAKPAASDRAELSAPAAATSRERPLGGPLARPVGA
                     EGRGEARRFARLLASEIKLYNEREVAEGRARGDLYARLRADIERGRRLYEERIPADLR
                     SGADYYYEELVEVLAGGRAEALR"
BASE COUNT          298 a          734 c          732 g          265 t
ORIGIN      
        1 ggcgcgggag cgcgacccgt cgtgccgtga gttcgccggc gggagaagag cgcgtacaga
       61 tcgggggggt cgcccgccgc gagccgggca tagatcgcgc ccgcctcgtc cttccggttc
      121 tccttttcca ggcatcgcgc gcgccagtac gcgagccgac ggtcgcgcca gatgtcgttg
      181 taggtcgccg cgagcgcctc gaaccggcgg cgcgcgccgg ggaaatcgcg cgagagccaa
      241 ttcttccacg cgagctccca gatcggctcg aagccgcgca ccgagccgcg cgtctgcgac
      301 gtgagctgcc gggccagggc gagcgcaccg tcgaagtctt cggcttccgc ggccaggcgg
      361 acgagccgct cccacgccgc ggctcgcgtc ggcgcgggtg cggaagcgac ggcgacgctc
      421 tcgagcatcc ggcggaccgg aacgatccgg ggggtcttcg cgaggagagc accgtccgcc
      481 ttctttcgaa gccgctcgag gaggagatcc gcgcggaaga gcgtcgcctc gaaccactgc
      541 ggcgtccggc gcgggacgcg cgccgcttcg gcgtcggcgg cgccggagcg gccgagcgcg
      601 gtcagcgccc gggcgagagc gaggtggttc gaaggtcggt cctccggcgc ccacgccccc
      661 gggcgctcca ggaggagccg ttgaccgcgg cgcgaatcgc cggcagcgac gagagcacgg
      721 ccgcgtcgcg ctcgatcggc agcggacagg cgtttctcct cgcgcttcaa cgcctcgcga
      781 acagactccg gcgtcgagga gaccgcgtcg agcgccggcg cgcggatgag gagagcatgc
      841 gcggcggacg tcactgtctg cacgtctccg atcgtcaacg cgaggcggat ccgctcgact
      901 ccgatctcgg caaagttgct tccaggcgcg gaggcggcga cgccgttcag caccgcgatg
      961 gccacgcgca cccgtccgga cacctcgagc gcgcgtgcga gatcccgcgc gaccgcggtc
     1021 cggcgggcga acggctccgc cgactcgaga gcgccgcgcc actccttgat tgcggcctcc
     1081 cgccggccgg cggcttcgag gacccgcccg agccaggcgc gccgatacgg ctcgaggcca
     1141 atcgcagccg cttccgcggc gcgcagattt ttctcggcgg acgcgaagtc cttgcgccgc
     1201 tcgtcgtcct cggcgagcag cagggagatc cgcgcgtgcc acgcacgctg ccccgccttc
     1261 cgcgcgatct cggcgagaac ggtcgcggcg gtggtccggt cccctgcctc gagcagatcc
     1321 gccgcgcgcg cgaactccgc gtcgaggtcc gaagtcaccg ggcgcatggg gatatccgca
     1381 gccgccgtgg atgcgatcag aaaaagcgcg aggcaggctc ggaatgggac catctcgagg
     1441 aaaaccgttt tccgacgagg aagattcggc gagacctatc gaagtgcttc cgctctcccc
     1501 ccggccagga cctccacgag ctcctcgtag taatagtccg cgcccgaacg gaggtccgca
     1561 ggtatccgct cttcatagag ccggcgaccg cgctcgatgt cggcgcgcaa gcgggcgtac
     1621 agatcgccgc gcgcgcgtcc ttccgccacc tcccgctcgt tgtagagctt gatctccgaa
     1681 gcgaggagcc gcgcaaaacg acgcgcttcg ccgcgcccct cggcgccgac cgggcgtgcg
     1741 agcggaccgc cgagtggccg ttcccggctc gtggcggcag cgggggcgga aagttccgca
     1801 cggtcggacg ccgccggctt cgccggtgtc gcagtgcttt cgtcgctcgg aagaggggcc
     1861 gcgggccgct ccacagaagg ggtcgaggcg aacgacggcg aaacgtccgc ctccgctgcc
     1921 tcttccgcgc cacggaaggc gcgcagcgca ggcgaaggcg cgagcttcct cacggcgatg
     1981 cggtcgacgg cgagcccggc gatccacgtc aggagcgcca ccgcgtccg
//
LOCUS       DASWAV010000596         1853 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U2665
            k141_195663, whole genome shotgun sequence.
ACCESSION   DASWAV010000596 DASWAV010000000
VERSION     DASWAV010000596.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960316
            Sequence Read Archive: SRR10260007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 1853)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1853)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:47:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,929
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1853
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_195663"
                     /isolate="SMAG_U2665"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.36444 N 132.31889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..507)
                     /locus_tag="VF958_12375"
     CDS             complement(<1..507)
                     /locus_tag="VF958_12375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014198457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlyA family RNA methyltransferase"
                     /protein_id="HEX9149951.1"
                     /translation="MPRTRLDLLLVARGLAPTREKAQAMILSGGVSVDGRRVDKAGQA
                     VEETASVAVAGPPHPYVSRGGLKLAAALDAFRLDPTGLVCLDVGASTGGFTDCLLQRG
                     AARVYAVDVGYGQLDSKLRGDPRVVLRERVNARFLSSEHVPEPVAIGVVDVSFISTRL
                     VLPAVVPLV"
     gene            complement(518..1528)
                     /locus_tag="VF958_12380"
     CDS             complement(518..1528)
                     /locus_tag="VF958_12380"
                     /inference="COORDINATES: protein motif:HMM:NF012335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HEX9149952.1"
                     /translation="MKAVRVERDGSLSVVELPVPRIGPGDALMRTRAAGICGSDLLNW
                     YVRRKAGTILGHEVAGEIIEMGGTVPGFAPGDRVAPHHHAACGECAECRSGRAVHCRE
                     WRASALDPGGMAEFVRVPAANLARDTLKVPSPVSDEEATFVEPLATVVKAFGRGGFAP
                     TQSLLAVGLGTTGQLAIRLARSGGALRVAGADRVASRLDLARASGATEVFDVSREPLE
                     KSVEDPFDFVFVGPGKAAVIESALAAVTPGGTLLAFTMASPEERLTLSPHDLYFREVR
                     IVPSYSAGPPEMREALAILAARRVGVADLVTHRFPIERAPDAFARAAQPEGSLKVLLT
                     FA"
     gene            complement(1525..>1853)
                     /locus_tag="VF958_12385"
     CDS             complement(1525..>1853)
                     /locus_tag="VF958_12385"
                     /inference="COORDINATES: protein motif:HMM:NF012335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HEX9149953.1"
                     /translation="MAVDCTGDPEVWRRLADLVVPGGRVLLFGGCAPGTSVTWDAARL
                     HYSEISLVGSFHYTPDEARAALAMLASGEIDPAPLITERGSLSDLPRFLDAQARREGI
                     RYAVIP"
BASE COUNT          314 a          650 c          665 g          224 t
ORIGIN      
        1 gacgagcggc acgaccgcgg gcaacacgag ccgcgtcgag atgaacgaga cgtcgacgac
       61 cccgattgcg acgggctccg gcacgtgctc ggacgagaga aaccgggcgt tgactctttc
      121 gcgcaggacg acgcgcgggt cgccgcgcag cttcgaatcg agttgaccgt agcccacgtc
      181 gaccgcgtag acccgcgccg cgccgcgctg gagcagacag tccgtgaatc ctccggtcga
      241 cgcaccgacg tcgaggcaga cgaggccggt gggatcgagt cggaaagcgt cgagcgccgc
      301 cgcgagtttc aggcctcccc gggaaacgta cgggtggggc ggaccggcga cggccacgga
      361 cgccgtctcc tccaccgcct gacccgcctt gtcgacgcgc cggccgtcga ccgaaactcc
      421 tccggagagg atcatcgcct gcgccttctc ccgcgtcggc gcgaggccgc gtgcgacgag
      481 caggagatcg agccgcgtgc ggggcatcgg ggaggtctca ggcgaaagtg agcagaacct
      541 tgagcgaccc ctccggctgc gcggcgcgcg cgaaagcgtc gggcgcccgt tcgatcggaa
      601 agcggtgcgt cacgaggtcg gcgacgccga cgcgccgcgc cgccaggatg gcgagcgcct
      661 cccgcatctc gggggggccc gcggaatagg aaggcacgat ccgaacctcg cggaagtaga
      721 ggtcgtgcgg ggagagggtc agccgctcct cgggcgaggc catggtgaac gcgagcaacg
      781 tcccgcccgg agtgacagcg gcgagcgcag attcgatcac cgcggccttg cccggcccga
      841 cgaagacgaa gtcgaacggg tcctcgacgg acttctcgag aggctcgcga gagacgtcga
      901 acacctccgt ggctccgctc gctctcgcca aatcgagccg cgaagccacg cggtcggccc
      961 ccgcgacccg caacgcgccg ccggaccgtg cgaggcggat cgcgagctgg cccgtcgtgc
     1021 cgaggccgac ggcgagaagc gactgcgtgg gagcgaatcc cccgcggccg aacgccttga
     1081 cgactgtcgc gaggggctcg acgaaggtgg cttcctcgtc cgagacgggg gacggaactt
     1141 tcagggtgtc gcgcgccagg ttcgccgcag gaacccgtac gaactccgcc atcccgcccg
     1201 gatcgagtgc ggaagcgcgc cactcgcggc agtggacggc gcggccgctc cggcactcgg
     1261 cgcactcgcc gcacgccgca tggtggtggg gcgcgacgcg gtctcccggc gcgaacccgg
     1321 ggacggtgcc ccccatctcg atgatctcgc ccgcaacctc gtgtccgagg atggtccccg
     1381 ccttccggcg cacgtaccag ttgaggaggt cggagccgca gattccggcc gctcgcgtgc
     1441 gcatcagcgc gtcacccggc ccgatccgcg gcacgggcag ctcgacgacg gaaagggatc
     1501 cgtccctctc gacccggacg gccttcacgg aatcaccgca taccggatcc cttcacgccg
     1561 cgcctgagca tcgaggaacc ggggcagatc ggagagcgag ccgcgttcgg tgatcagcgg
     1621 agccggatcg atctcgcccg atgcgagcat ggcgagcgcg gcgcgcgcct cgtccggcgt
     1681 gtagtgaaac gagccgacga gcgagatctc cgaataatgc agccgggccg cgtcccatgt
     1741 gacggaggta cccggagcgc atccgccgaa gagaaggact ctgccgccgg gcacgacgag
     1801 gtcggcgagg cgccgccaga cctccggatc gccggtgcag tccaccgcca tgt
//
LOCUS       DASWAV010000597         1986 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U2665
            k141_589925, whole genome shotgun sequence.
ACCESSION   DASWAV010000597 DASWAV010000000
VERSION     DASWAV010000597.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960316
            Sequence Read Archive: SRR10260007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 1986)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1986)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:47:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,929
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1986
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_589925"
                     /isolate="SMAG_U2665"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.36444 N 132.31889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..437
                     /locus_tag="VF958_12390"
     CDS             <1..437
                     /locus_tag="VF958_12390"
                     /inference="COORDINATES: protein motif:HMM:NF012780.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEX9149954.1"
                     /translation="RNAEGRLAGVITDRDICMAAARNGRPAREIRVREAMTTEVLTCS
                     RDEDVLEAVSRMEQGHVHRLPVVSREGELEGVLSIDDAIDHAGEAWEQPLSGVSLGEV
                     VPVLQALHERIVRHPAWEEKTNESGTQHDSTGPAQVREAPEH"
     gene            448..1125
                     /locus_tag="VF958_12395"
     CDS             448..1125
                     /locus_tag="VF958_12395"
                     /inference="COORDINATES: protein motif:HMM:NF016836.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BON domain-containing protein"
                     /protein_id="HEX9149955.1"
                     /translation="MLETFQKTDLQIQQDVMRELGWDTRIGPAKVGVEVDKGIVTLTG
                     TLDNFAKKHAATEAAHRVAGVLDVANDILVHLAGSPGKTDTEIARAVRSALEWDAFVP
                     DQRIRSNVSDGWVTLEGDVEYLRESEDAARVVRRLDGVKGVWNQIIVKPKKVEPAAVR
                     KMIEEALERRAEREAEQIGVAIENGRVTLSGKVHSWLEKDAVVGVVGHAPGVQTVNDK
                     LRIEPWS"
     gene            1384..>1986
                     /locus_tag="VF958_12400"
     CDS             1384..>1986
                     /locus_tag="VF958_12400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018912562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-binding domain-containing protein"
                     /protein_id="HEX9149956.1"
                     /translation="MHPEVRRSGPDACPKCGMALEPVAPAAPAARTLWTCPMHPEILR
                     DAPGSCPICGMALEPRTVVSAEEPENPELADMTRRFKVSAALTAPLVLLAMAHLVPGN
                     PLDHAIPHRTRALLELALATPVVLWGGWPFFVRGWYSLVHRALNMFTLIGLGIGVAYV
                     YSVVAALFPGVFPASFRGPGGEVGLYFEAAAVIVTLVLLGQ"
BASE COUNT          330 a          657 c          693 g          306 t
ORIGIN      
        1 ttcgcaacgc cgagggccgg ctcgcaggag tgatcacgga ccgcgacatc tgcatggcag
       61 ccgcgcggaa cggccggccc gcccgtgaaa ttcgggtacg ggaggcgatg acgaccgaag
      121 tcctgacgtg ctccagggac gaggacgtac tcgaggccgt cagcaggatg gagcagggtc
      181 acgtccaccg cctgcccgtg gtgtcgcggg aaggagaact ggagggtgtc ctttcgatcg
      241 acgacgccat cgaccacgcc ggcgaggcct gggagcagcc gctctcgggc gtctcgctcg
      301 gggaggtcgt tccggttctc caggcgctgc acgagcgaat cgtccggcat ccggcctggg
      361 aggagaaaac gaacgaatcc ggcacgcaac acgactcgac cggccccgcg caggtgcgcg
      421 aggcgcctga acactgagga ggaagtcatg ctcgaaacgt tccagaaaac agatctgcag
      481 atccagcagg acgtgatgcg cgagctgggg tgggacacgc ggatcggtcc ggccaaagtc
      541 ggcgtcgagg tcgacaaggg aatcgtgacg ctgacgggca cgctggacaa tttcgcgaag
      601 aaacacgcgg cgacggaagc cgcccaccgc gttgccggcg tcctcgacgt cgccaacgac
      661 atcctggtgc atctcgccgg aagccccggg aagacggaca cggaaatcgc ccgggcggtt
      721 cgtagcgccc tcgaatggga cgcgttcgtg cccgatcagc gaatccgctc gaacgtctcc
      781 gatggctggg tgacgctcga aggggacgtg gagtacttga gggagagcga agatgcggcg
      841 cgggtcgtcc ggcggctcga cggcgtcaag ggggtctgga accagatcat cgtgaagccg
      901 aaaaaggtcg agcccgcggc agtccgcaag atgatcgagg aagcgttgga gcgccgcgcg
      961 gagcgcgagg ccgaacagat cggcgtggcg atcgagaacg gccgggtgac gctttcgggc
     1021 aaggtccact cctggctcga gaaggacgcg gtcgtcggcg tcgtcggcca cgctcccggc
     1081 gtccagaccg tgaacgacaa gctccgtatc gagccctggt cctagctgga aacggtcctc
     1141 acggaccgat tgccgccgcg gttccgtacc ttccacggag ccgcggcgga atattttttg
     1201 ttgcgcgctt cggaagatcc cgggcccgag gtccgggccc ggagcgtgaa gccctggcgg
     1261 tcgcgcgagc gtatacgcta ggccgcaatg acgtcgccac ggcttcccat gctgccgagc
     1321 catccggggt ctcagccgcc gcgagaagcg gccctctcgt ccccttccga ttacacctgt
     1381 ccgatgcacc cggaggtacg ccggtcggga ccggacgcct gcccgaaatg cggcatggcg
     1441 ctcgagccgg tcgcacccgc cgctccggcc gcgcgtacgc tctggacctg ccccatgcac
     1501 ccggagatcc tgcgggacgc accgggcagc tgcccgatct gcgggatggc gctggagccc
     1561 aggacggtcg tttctgcgga ggagccggag aatcccgagc tcgccgacat gacccggcgt
     1621 ttcaaggtga gcgcggccct gaccgcgccg ctcgtgttgc tcgccatggc ccacctcgtc
     1681 ccgggcaacc cgctcgacca cgcgatcccg caccgcaccc gcgcgcttct cgagctcgcg
     1741 ctcgcgacgc cggtcgtgct gtggggaggc tggcctttct tcgtgcgcgg ctggtactcc
     1801 ctcgtgcacc gcgccttgaa catgttcacg ctgatcggtc tcgggatcgg cgtggcgtac
     1861 gtctacagcg tcgtcgccgc gctctttccc ggcgttttcc cggcttcctt tcgcgggccg
     1921 ggcggcgaag tgggcctcta cttcgaggcc gcggccgtga tcgtcacgct cgtgctgctc
     1981 gggcag
//
LOCUS       DASWAV010000598         3194 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U2665
            k141_589966, whole genome shotgun sequence.
ACCESSION   DASWAV010000598 DASWAV010000000
VERSION     DASWAV010000598.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960316
            Sequence Read Archive: SRR10260007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 3194)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3194)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:47:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,929
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3194
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_589966"
                     /isolate="SMAG_U2665"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.36444 N 132.31889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..160
                     /locus_tag="VF958_12405"
     CDS             <1..160
                     /locus_tag="VF958_12405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004619536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HEX9149957.1"
                     /translation="LNEKKLSVNETAYLVGFSDPAAFSRAFKRWTGSSPGTMRASKPG
                     KGKSRTSD"
     gene            complement(258..1055)
                     /locus_tag="VF958_12410"
     CDS             complement(258..1055)
                     /locus_tag="VF958_12410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="cache domain-containing protein"
                     /protein_id="HEX9149958.1"
                     /translation="MLRLSSILVVAGVSSLAGGAATLAQPTANVQRAAPTFTVDGRIA
                     LHSFMALSDAHLKKLADLLDLLATTDDARSGEWERIRGPLAGLTPITVPAVLWFAQSD
                     GSYWTVAEGRAAGKLSDRAYFPRVLSGQTVMGDLVVSRSTNRNTAIVAVPVRNRGNTV
                     VGVLGASVHLDRLGELVRQEMGGLGDDLLFFAIDSTPRGALHSDPTMVFTEPMRLGDE
                     GMRKAFTEMLSRTEGTVNYTFRGRPRTVLYRKSPVTGWWYAMGILQK"
     gene            complement(1286..>3194)
                     /locus_tag="VF958_12415"
     CDS             complement(1286..>3194)
                     /locus_tag="VF958_12415"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007404092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="M3 family metallopeptidase"
                     /protein_id="HEX9149959.1"
                     /translation="DVIAENPEPPTFENTFVPLEDAGRHSDRAQTMFSILTNNLSTPE
                     AQAVDREWSPKIAAAYDGITFNTKLFSRVQTIYDGKGSLNPEQRRLAELNYDRFLRAG
                     AKLSRDDKAKVGKINEELAGLFSDFSKKVLADENTWTVLESKKDLAGLSPSLVATYKA
                     AADERNLAGKWAVVNTRSSVDPFLASSSRRDLREKVWKAFKNRGDNGGENDTNAVIAR
                     IVKLRADRARLLGFPTHAHWRMADTMAKDPEKAKALMMRVWPAAVARVREEVRDMDKF
                     AKRDGVKVFEPWDYLYYAEKVRKAKYDLDQDELKPYFELDHMIAGAFYMAEQLYGLTF
                     TEITGTVPVFLEGVRVWEVKDKATGAHVGLFYGDYFARKGKQSGAWASGYRGHETFTG
                     KTITPITSNNNNFVKGSAGEPILISLDDAQTLFHEFGHAIHALVSEVHYPGLATTPRD
                     FVEYPSQVHEMWVLTRPILDRFARHYKTGMPMPDALVKKVEASRRFNQGYATVEYLSS
                     AIVDMELHTKPDGVVDPDAFERETLQRIGAPREVAMRHRLPQFNHLFTSDSYSAGYYS
                     YLWSEVMDADTREAFVEAGNVFDPTVAGKMRKFILAPGNSTDRAEAFRQFRGRDPDVT
                     ALLKKRGFPAN"
BASE COUNT          515 a         1042 c         1037 g          600 t
ORIGIN      
        1 cctgaacgaa aagaagctct cggtgaacga gacggcctat ctcgtgggct tctcagatcc
       61 tgccgcgttc tcgcgcgcgt tcaagcgttg gaccggatcg agcccgggca caatgcgcgc
      121 ctcgaaaccg gggaagggca agtctcggac gtcggactga tccctcggca aggcgagagc
      181 cggcggtacc tgcgaccagt cgcgccgcca cgcgtcggat tccgcgcgcc caccggcccc
      241 tcagccgccg cctaccctca cttttgcagg atccccatcg cgtaccacca tcctgtgacc
      301 ggtgactttc ggtagagcac cgtgcgtggg cggccgcgga aggtgtagtt cacggttccc
      361 tcggtacgcg acagcatttc ggtgaacgcc ttccgcatgc cttcgtctcc cagccgcatc
      421 ggctcggtga acaccattgt cggatccgag tgcagcgcgc ccctcggcgt ggagtcgatg
      481 gcgaaaaaga gcagatcatc tcccagacct cccatttcct gccgtaccaa ctcgccgagg
      541 cggtccagat gtacggaggc gcccagcacg ccgacgacgg tgtttccgcg gttgcgcacc
      601 ggaaccgcca cgatcgcggt gttgcggttc gtggagcgac tgaccaccag atcgcccatg
      661 accgtctgtc ccgagagcac ccgggggaag tacgcccggt cggagagctt gccggcggcg
      721 cgcccctctg cgacggtcca gtaggatcca tcggactggg caaaccagag tacggcgggc
      781 acggtgatcg gcgtcaggcc ggccagcggg ccacggatgc gctcccattc ccccgagcgg
      841 gcgtcgtcgg tggtggcgag gagatcgagc aggtccgcca gcttcttcag gtgtgcgtcg
      901 ctcaacgcca tgaatgagtg aagcgcgatc cgtccatcga cggtgaacgt cggcgccgcg
      961 cgttgcacat tcgcggtcgg ctgcgccagg gttgcggcgc ctcctgcaag cgaactcacg
     1021 ccggccacca cgaggatcga ggagagccga agcacgaatc tcttggctct agacatttgt
     1081 cgtctcctat cgaacgtcgg cgggttcgga gcagctctgg cgtgcagcta gagggccaag
     1141 ctctcctggg ggccgcccga cggtgctcgt gagagcgcac cgagcctagc gcgccgggtg
     1201 attcgccgcg tggtgtggat taaccagctt cgaatgcaga acttcgccac gtcctcgcga
     1261 gccgagagca ctcggtccgg cgtgatcagt tcgcgggaaa gccgcgcttc ttgagcagtg
     1321 ccgtgacatc gggatcgcgg ccgcggaact ggcggaaggc ctcggcgcga tccgtcgagt
     1381 tccccggcgc gaggatgaac tttcgcatct ttccggcgac ggtcggatcg aacacgttgc
     1441 ccgcctcgac gaacgcttcg cgcgtatcgg cgtccatgac ctcggaccag aggtaactgt
     1501 agtagcccgc cgagtacgag tcgctggtga agaggtggtt gaactgaggc aggcggtgcc
     1561 gcatggcgac ctccctcggc gctccgatcc tctgcagggt ctcgcgctcg aacgcgtccg
     1621 gatcgaccac gccgtccggc ttcgtgtgca actccatgtc gacgatcgcc gacgacaggt
     1681 actcgaccgt ggcgtagccc tggttgaacc tgcgcgaggc ttcgaccttt ttcacgagcg
     1741 cgtccggcat cggcatcccg gtcttgtagt gccgggcgaa ccggtcgagg atcggccgcg
     1801 tcagaaccca catctcgtgg acctgcgacg ggtactcgac gaagtcgcgc ggagtggtcg
     1861 cgaggccggg atagtgaacc tcggaaacca gcgcgtggat cgcgtggccg aactcgtgaa
     1921 aaagggtctg cgcgtcgtcg agcgagatca ggatcggctc cccagcgctc cccttgacga
     1981 agttgttgtt gttcgacgtg atcggcgtga tcgttttgcc ggtgaatgtc tcgtgcccgc
     2041 ggtaacccga ggcccacgcg ccggactgtt tccccttgcg ggcgaagtag tcgccgtaaa
     2101 acagtccgac gtgagcgccg gtcgccttgt ccttgacctc ccacacgcgg acgccctcga
     2161 ggaaaacggg caccgttccg gtgatctcgg tgaaggtcag cccgtacagc tgctcggcca
     2221 tgtagaaagc gcccgcgatc atgtggtcga gctcgaagta cggcttcaac tcgtcctggt
     2281 cgagatcgta cttcgccttg cggactttct cggcgtagta caggtagtcc cagggctcga
     2341 agaccttgac cccgtcgcgt ttcgcgaact tgtccatgtc ccggacttcc tcgcgaacgc
     2401 gggcgaccgc cgccggccag acgcgcatca tcagagcctt cgccttctcg gggtctttgg
     2461 ccatcgtgtc cgccatgcgc cagtgggcgt gggtgggaaa ccccaggagc ctggcccggt
     2521 ccgcgcgcag cttgacgatg cgtgcgatga ccgcgttcgt gtcgttctct ccgccgttgt
     2581 cgccgcgatt cttgaacgct ttccagacct tctcacgcag gtcgcggcgc gacgaggacg
     2641 ccaggaacgg gtcgacgctc gatcgcgtgt tgacgaccgc ccacttgccc gcgaggttgc
     2701 gctcgtcggc cgctgccttg tacgtcgcga cgagcgacgg ggagagcccc gcgagatcct
     2761 tcttcgattc gagcaccgtc cacgtattct cgtcggccag aaccttcttg ctgaagtcgc
     2821 tgaacagccc ggcgagctcc tcgttgatct tcccgacctt cgctttgtcg tcgcgggaca
     2881 gcttcgcgcc tgcgcggagg aatcggtcgt agttcagctc ggcgagccgg cgctgctcgg
     2941 ggttcaaact ccccttcccg tcgtagatcg tctggacccg cgagaagagc ttcgtgttga
     3001 acgtgatccc gtcgtaggcg gcggcgatct tcggcgacca ttccctgtcg accgcctggg
     3061 cctcgggcgt gctgaggttg ttcgtcagga tcgagaacat cgtctgagcg cgatcggaat
     3121 ggcgcccggc gtcctcgagc ggcacgaaag tgttctcgaa cgtcggcggc tcggggtttt
     3181 ctgcgatcac gtcg
//
LOCUS       DASWAV010000599         1941 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U2665
            k141_792608, whole genome shotgun sequence.
ACCESSION   DASWAV010000599 DASWAV010000000
VERSION     DASWAV010000599.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960316
            Sequence Read Archive: SRR10260007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 1941)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1941)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:47:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,929
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1941
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_792608"
                     /isolate="SMAG_U2665"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.36444 N 132.31889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..787)
                     /locus_tag="VF958_12420"
     CDS             complement(<1..787)
                     /locus_tag="VF958_12420"
                     /inference="COORDINATES: protein motif:HMM:NF014839.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TGS domain-containing protein"
                     /protein_id="HEX9149960.1"
                     /translation="MLAENVTIRLPDGATLEVPKGTTVRGLAERIGPRLAKDAVAGKL
                     DGRIVDLITPLDADASVEIITSKSPDVLPIYRHSTAHLTAQAVKRLFPDVQIGIGPPI
                     ENGYYYDFLPQRPFTPEDLEEISEEMRRIIGEDLPITRAEMPVSEAVSIFEGQNDPLK
                     VQIARELPDEFVSCYRQGEFIDLCRGPHVASTGRLGVFKLTHTAGAYWKGDEKNPMLQ
                     RIYGASFLTQKELDEYLKRLEDAKARDHRKLGRELELFTFHPLA"
     gene            complement(867..942)
                     /locus_tag="VF958_12425"
     tRNA            complement(867..942)
                     /locus_tag="VF958_12425"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(907..909),aa:Val,seq:cac)
     gene            complement(958..1032)
                     /locus_tag="VF958_12430"
     tRNA            complement(958..1032)
                     /locus_tag="VF958_12430"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(998..1000),aa:Val,seq:gac)
     gene            1288..>1941
                     /locus_tag="VF958_12435"
     CDS             1288..>1941
                     /locus_tag="VF958_12435"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX9149961.1"
                     /translation="MSSSLRASLSSLARRWTTFREERAETAAARRFWSGETAREAEDH
                     YWGAQPLVRRAINRRVTGDPNRWPMEWFRETYARQPFARGLSVGCGAGLLERDVLAKG
                     ICEAMEGIDFSEESLEQARFAAEEEHLSDRLEYRAEDINALKLPRARYDIVFFHGSLH
                     HIRQVERVLTEVRQALIPGGLVYLDEYMGPSRSEWTDDGWGFARSAFDSLPEELKNRP
                     "
BASE COUNT          327 a          619 c          644 g          351 t
ORIGIN      
        1 aagcgagcgg gtgaaacgtg aagagctcga gctcccgtcc gagcttgcga tggtcgcgcg
       61 ccttcgcgtc ctcgagccgc ttcaggtact cgtccagttc cttctgcgtc aggaacgacg
      121 ccccgtagat ccgctggagc atcgggttct tctcgtcgcc cttccagtac gcgccggccg
      181 tgtgagtcag cttgaagacg ccgagccgcc cggtcgaagc gacgtgtgga ccacggcaga
      241 ggtcgatgaa ctcaccctgc cggtaacagg agacgaactc gtcgggaagc tcccgcgcga
      301 tctggacctt caggggatcg ttctgtccct cgaaaatcga gacggcctcg gagacgggca
      361 tctcggcgcg ggtgatcggg aggtcctccc cgatgatgcg acgcatctcc tcggagatct
      421 cctcgaggtc ctcgggagtg aacggccgct gcggcagaaa gtcgtagtaa tagccgttct
      481 cgatcggtgg accgatgccg atctgtacgt ccgggaagag cctcttgacc gcttgcgccg
      541 tcaggtgcgc ggtcgagtga cggtagatgg gaaggacgtc cggagacttc gaggtgatga
      601 tctcgacgga cgcgtcggcg tcgagcgggg tgatcaggtc gacgatgcgt ccgtcgagct
      661 tgccggcaac ggcgtccttc gcaaggcgcg gcccgatgcg ttccgcgagt ccgcggacgg
      721 tcgtaccctt cgggacctcg agggtcgccc cgtcgggcag ccggatcgtt acgttttccg
      781 ccagcacgtc catgttctcc cggttcccca gaattccttc cgcaaaaaac gaaaacgcgg
      841 gttaccccgc gtcgtctttc ttcttttggt aggcgctaac gggatcgaac cgttgacctc
      901 taccgtgtga aggtagcgct ctcccagctg agctaagcgc cttcgtttcc gaagaattgg
      961 taggctcgag cggatttgaa ccgctgaccc ctaccgtgtc aaggtagtgc tctccccctg
     1021 agctacgagc ctgttttcgt cccggtctga gcacgtcgcg gggacaggtc ccacggacct
     1081 gcacccgcat ccgaactttc gccgaggggc tacgggccgg aagcccgcgg gggcgtttca
     1141 tcccggcctt cgtccctgat tgaaaaagag cttctccgat cgagatcgac tcggccggag
     1201 gagtgcgcaa tctagcagac gcacgattcc gacgcaaacg tcggagcgga aaggcgctcc
     1261 ggctgctata ttgatttcaa aactcgaatg tcatcgtctc tccgagcgtc cctgtcctcg
     1321 ctggcgcgcc gctggacgac gttccgcgaa gagcgcgccg agacggccgc cgcccgccgg
     1381 ttctggagcg gcgagaccgc gcgcgaggcg gaggaccact actggggagc gcagccgctg
     1441 gtgcggcgcg ccatcaaccg gcgagtcacc ggcgacccga accgctggcc gatggagtgg
     1501 ttccgcgaga cgtacgcccg gcagccgttc gcccgcggct tgtcggtggg ctgcggggcg
     1561 ggcctcctcg agcgcgacgt cctcgccaag gggatctgcg aagcgatgga gggcatcgat
     1621 ttttccgagg agtccctcga gcaggcgcga ttcgcggcgg aggaggagca cctgtcggac
     1681 cgcctcgagt accgcgccga ggacatcaac gccctgaagc tcccgcgggc ccggtacgac
     1741 atcgtcttct ttcacgggtc gctccatcac atccggcagg tggagcgggt cctgacggag
     1801 gtccggcagg cgctcattcc cggagggctc gtctacctcg acgagtacat ggggccgtcg
     1861 cgatcggaat ggaccgacga cggctggggc ttcgcgcgaa gcgcgttcga ctcgctcccc
     1921 gaagagttga agaaccggcc g
//
LOCUS       DASWAV010000600         1920 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U2665
            k141_73211, whole genome shotgun sequence.
ACCESSION   DASWAV010000600 DASWAV010000000
VERSION     DASWAV010000600.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960316
            Sequence Read Archive: SRR10260007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 1920)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1920)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:47:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,929
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1920
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_73211"
                     /isolate="SMAG_U2665"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.36444 N 132.31889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            123..1895
                     /locus_tag="VF958_12440"
     CDS             123..1895
                     /locus_tag="VF958_12440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015351117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF885 domain-containing protein"
                     /protein_id="HEX9149962.1"
                     /translation="MKISALLSATLLLGLSCSPAPSTAPAPSRVSSAVPAAAAAGAAE
                     SFSKFVDDYFDAQFAYVPSQGTDAGFHEYDTKLEDRSRARIETRIGELKRFLSRLQAF
                     DRSKLPPDEAIDADALEGEIRSLLLDQETLRNWERNPMGYAALPGSAADSLIKRDFAP
                     APERLRSLVAREKQMPAVYAAGRANLVNPPKEFTDLAIRIAKGSAGYFSGTVAQWAKQ
                     AAGSDTALWKEFETTNAQVVAATRDFADWIEKDLLPRSKGSYAIGAANFLAKMKYDDM
                     VDLPLADLLAKGEAQLAKDHAAFVETAKRIDPSKTPAEVMKSLSNEHPTPETLIPAVA
                     QSVENARRFLVEKDLVTIPSEVRPHVEETPPFARSGSFASMDTPGAYETKATEAFYYV
                     TPVEKEWDAKHKEEHLRLYNPYVVSMIDVHEVWPGHYLQFLYAPRFPTKTRKLVFCGT
                     NAEGWAHYAEQMMVDEGFGAGDPKYRLAQLQEALLRDCRYVVGIKLHTQGMTVEDGAR
                     VFVEKGFQEPANAYEEARRGAYNPTYLYYTLGKLMIQELSAEYRAKNGASLKQFHDAF
                     VAQGGLPIPLVRRILFRAPAAASR"
BASE COUNT          370 a          637 c          604 g          309 t
ORIGIN      
        1 acggacgaac gagcgcataa ccatcctcct ggaaaggcga ggagaaccct atttcgtcgc
       61 ctcgtgaatc gcaatggagc tcgaaccgcc cggaggcgtc gagtcgatat agcctttcga
      121 gcatgaagat ctcagcgctt ctctcggcaa ctctcctcct cggtctttcc tgctcgcccg
      181 cgccgtccac tgcgcccgct ccgtcacggg tctcgtcggc cgttccagcc gcggctgcgg
      241 caggagcagc cgaatcgttc tccaagttcg tcgacgacta tttcgatgcg cagtttgctt
      301 acgtgccctc gcagggaacc gacgccggtt tccacgagta cgacacgaag ctcgaagacc
      361 ggtcgcgcgc ccgtatcgag acgcgtatcg gggagctgaa acggtttctt tcccgcctgc
      421 aggcgttcga ccggtcgaag ctccctcccg acgaggcgat cgacgcggac gcgctcgagg
      481 gcgagatccg cagcctcctg ctcgaccagg aaactctgcg caactgggag cgcaacccga
      541 tggggtacgc ggcgctgccc ggcagcgcag cggacagcct gatcaagcgc gacttcgcac
      601 cggcgcccga gagactccgg tcgctcgtcg cgcgcgagaa gcagatgcct gcggtatacg
      661 cggccggcag ggcaaatctc gtcaatccgc cgaaagaatt caccgacctc gcgatccgca
      721 tcgcgaaggg gtcggccggt tatttttccg gaaccgtggc gcagtgggcg aagcaggcgg
      781 ccggcagcga cacggcgctc tggaaagagt tcgagacgac gaacgcgcag gtcgtcgccg
      841 ctacgcggga cttcgcggac tggatcgaga aggatctcct ccctcgctcg aagggaagct
      901 atgcgatcgg cgccgcgaac ttcctcgcga agatgaaata cgacgacatg gtcgacttac
      961 ccctcgccga cctcctcgcg aaaggggaag cccagctcgc gaaggatcac gccgcgttcg
     1021 tcgagaccgc gaagcggatc gatccgtcga agacgccggc ggaggtgatg aagtcccttt
     1081 cgaacgagca tccgacgccg gagacgctga tccccgcggt cgcgcagtcg gtcgagaacg
     1141 cccgacggtt cctcgtcgag aaagatctcg tcacgattcc ttcggaggtg cgtccgcacg
     1201 tcgaggaaac cccgccgttc gcgcgctccg gaagcttcgc ctcgatggac acgccgggag
     1261 cgtacgaaac gaaggcgacc gaggccttct attacgtcac gccggtcgag aaggagtggg
     1321 acgcgaagca caaggaggag cacctccggc tctacaaccc gtacgtcgtc tcgatgatcg
     1381 acgtgcacga agtgtggccc ggccactatc tccagttcct ctacgcgccg cgtttcccga
     1441 cgaagacccg caagctcgtt ttctgcggaa cgaacgcgga aggatgggct cactacgccg
     1501 agcaaatgat ggtggacgag ggcttcggcg ctggcgatcc gaaataccgc ctcgcacagc
     1561 tccaggaagc gctgctgcgc gactgccgct acgtcgtcgg gatcaagctg cacacgcagg
     1621 ggatgacggt ggaagacggt gcgcgcgtct tcgtcgagaa aggcttccag gaaccggcga
     1681 acgcgtacga agaggcgcgg cgcggcgcct acaacccgac gtacctgtac tacacgctcg
     1741 gcaagctcat gatccaggag ctctccgccg aataccgggc gaagaacggc gcctcgctga
     1801 agcagttcca cgacgcgttc gtcgctcagg gcggacttcc gatccccctc gtccgccgga
     1861 tcctgttccg ggccccggcg gcggcctcgc gttgaccgag ccacggccgt tttcgaccgc
//
LOCUS       DASWAV010000601         3659 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U2665
            k141_590197, whole genome shotgun sequence.
ACCESSION   DASWAV010000601 DASWAV010000000
VERSION     DASWAV010000601.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960316
            Sequence Read Archive: SRR10260007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 3659)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3659)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:47:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,929
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3659
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_590197"
                     /isolate="SMAG_U2665"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.36444 N 132.31889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..840)
                     /locus_tag="VF958_12445"
     CDS             complement(<1..840)
                     /locus_tag="VF958_12445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9149963.1"
                     /translation="MVNRTEDLETLLARIPDPEIRALFTPEFILCSEEFDRFTVDTVL
                     NLVEELGLGEPLARGTTVPEVLESRGWAPRAEIPVAWFFRKLAAEEHLEVNGTGRDAV
                     FRARGPLPVGDPDRAEARARALDPRSAPPFSVVRAMIEHVPSFLRGEESGEDVLFSPA
                     KLPLWFEYFSNDNLLYQINNRLGAEAVVRALPVNRAATLVEIGGGSGSAALAVAERLA
                     RDAALSRIGRYVFTEIVPTFLRRAERNVRSRFPLLPAEFSRLDMNRDFSEQGIEPGSA
                     DVLY"
     gene            889..2979
                     /locus_tag="VF958_12450"
     CDS             889..2979
                     /locus_tag="VF958_12450"
                     /inference="COORDINATES: protein motif:HMM:NF013799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HEX9149964.1"
                     /translation="MHFVAAAIVAGALSTAPPSRVVVLAFDGVDAGLVETMLAAGRLP
                     NLAALKARGGFSPLTPPVPPQTPVSWTSFATGLDPGGHQIFDFLKRDPANMTPTFAVA
                     EEKEVPFLLGKKTPVAAGAASAALFLVPAAVLAAKRRRIASGVLFLFAAGAGAASFTA
                     ARAWLPATRPSVESNRRGETFWTEAASRPATVIRMPVTFPPEPFRDGRLLSGLGVPDL
                     SGRIGKPAYYTSDPFFAPREGNDFSIELVRLVSNVGRQTTRIVGPPGRAFGRKGEIEL
                     PMTLTVPDSRDRIEIEAGRSRVTISPGQWSEWTSLDFRVNPLVTVHGYARFLLESASP
                     EIALYVSPIQFDPERLPPGFEISTPPDYARELVSRFGRYKTMGWAIDTWSIQSGTLSE
                     PEFLEDVAATVAHERRMLAELVKEGRRLLVYYFEFPDRVAHVFWRFRDPKHPAYDAAL
                     AAKYGDVVEKAYETMDAIVGETVRALPTGAALMVLSDHGFATWRRSVNYDSWLVEKGY
                     LVLKGDARRQNLEALFSRGAFWEAVDWSRSRAYAMGLGDLYVNLRGREKQGIVAPGAE
                     YEALREELARGLLELTDPATGERAVSRVFKREDVYRRYDPRLIPDMIVANRPGYRVSW
                     QASLGVPTGTVFEDNRDVWSGDHCSLDPDLVRGVFFASRPFSAPRVPTITDATAALRA
                     LLGGTPAEGAIVWK"
     gene            2976..>3659
                     /locus_tag="VF958_12455"
     CDS             2976..>3659
                     /locus_tag="VF958_12455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9149965.1"
                     /translation="MNAIAPIVCALLLFGPPAAPVSRIASPESERQADLARLRSRIQS
                     LQGQLEDSRRRQATLSEELARLELQLEIAAQEKELLARLREDYAVRLEEIAAERRSAE
                     EAASRSRRDLLARARLLQRFGRFGYVRVLLEARDVPAFLESVSRLDSLARRDARLLTE
                     HREAQRRLESGLERERALKTETDRLYAESRREESRIASLKTERENLLERQRAVSASQR
                     LEVAALSDKA"
BASE COUNT          530 a         1306 c         1246 g          577 t
ORIGIN      
        1 gtacaggaca tccgccgagc ccggctcgat cccctgttcg ctgaagtcgc ggttcatgtc
       61 gagccgcgag aactccgccg gaaggagcgg aaaccgggag cgaacgttgc gctccgcgcg
      121 ccgcaggaag gtcgggacga tctcggtgaa cacgtagcgc ccgatgcggg acagcgctgc
      181 gtcgcgcgcg agccgctccg cgacggcgag ggccgccgac cccgagccgc cgccgatctc
      241 gacgagggtc gccgcgcggt tgaccgggag cgcacgcacg acggcctcgg cgccgagccg
      301 gttgttgatc tgatacagca ggttgtcgtt ggagaagtac tcgaaccaca gaggcagctt
      361 cgccggagag aagagcacgt cttcaccgct ctcctcgccc cgcaggaagc tcggcacgtg
      421 ctcgatcatc gcgcgcacga ccgagaacgg aggcgcggat cgcgggtcga gggcgcgggc
      481 gcgggcttcc gcgcggtcgg ggtcgccgac cgggagcgga ccgcgggccc ggaagaccgc
      541 gtcccggccc gtcccgttca cttccagatg ctcctcggcc gcgagcttcc ggaagaacca
      601 tgcgaccggg atctctgcgc ggggcgccca gccccgcgat tcgagcacct cggggacggt
      661 cgtgccccgg gcgagcggct cgccgagtcc gagctcctcg acgaggttca ggaccgtgtc
      721 cacggtgaag cggtcgaatt cttcggagca gagaatgaac tcgggcgtga agagcgcgcg
      781 tatctccggg tcggggatgc gggcgagcag ggtctcgaga tcctccgtgc ggttcaccac
      841 gaacggattc tatgagcggg gcgcgccccg cccgcttaca ctgactcggt gcacttcgtc
      901 gcggccgcga tcgtcgccgg ggcgctctcg accgctcctc cctcccgggt ggtcgtcctc
      961 gctttcgacg gcgtggacgc cggtctcgtc gagacgatgc tcgccgccgg tcgcctcccg
     1021 aacctcgcgg cgctgaaggc gcgcggcggc ttttcgccgc tcactccgcc cgttccccct
     1081 cagacgccgg tctcctggac ttcgtttgca acgggcctcg atccgggcgg acaccagatc
     1141 ttcgacttcc tgaagcgcga tccggcgaac atgaccccga ccttcgcggt cgccgaggag
     1201 aaggaggtcc cctttctcct cggaaagaag accccggtcg cggccggcgc tgcctcagcg
     1261 gcgctcttcc tcgtccccgc ggcggtcctc gccgcgaagc ggcgccgaat cgcctcgggc
     1321 gttctctttc tcttcgccgc cggcgcaggc gccgcgagct tcaccgcggc ccgcgcgtgg
     1381 ctgcccgcga cgcgcccgtc ggtcgagagc aaccggcgcg gcgagacgtt ctggaccgag
     1441 gcggcctcgc ggccggcgac ggtgatccgc atgccggtca cgttcccgcc ggagccgttc
     1501 cgggacgggc gtctcctgtc cgggctcggc gtgccggacc tttcggggcg catcggaaag
     1561 ccggcttact acacctccga cccgttcttc gcgccgcggg aaggcaacga cttctcgatc
     1621 gagctcgtgc gcctggtgtc gaacgtcggc cgtcaaacga cccgcatcgt cggtccgccc
     1681 ggtcgggcgt tcggccgcaa aggggagatc gagctcccga tgaccctcac cgttcccgac
     1741 tcgcgggatc gcatcgagat cgaagcgggg aggtcgagag tcacgatctc gccgggacag
     1801 tggagcgagt ggacgagcct cgacttccgc gtgaacccgc tcgtgacggt gcatggatac
     1861 gcccgtttcc tgctcgaaag cgcgtctccg gaaatcgcgc tctacgtctc gcccatccag
     1921 ttcgatccgg agcggctccc cccgggcttc gagatctcga cgcccccgga ctacgcgcgc
     1981 gagctcgtct cccgcttcgg acggtacaag acgatgggct gggcgatcga cacgtggtcc
     2041 atccagtcgg gcacgctctc cgaaccggag ttcctggagg acgtcgccgc aaccgtcgcg
     2101 cacgagcgcc ggatgctcgc ggagctcgtg aaggaaggcc ggcgcctcct cgtctactac
     2161 ttcgagttcc ccgaccgcgt cgcgcacgtc ttctggcgct ttcgcgaccc gaagcacccc
     2221 gcgtacgacg cggcgctcgc ggcgaagtac ggcgacgtag tcgagaaggc ctacgagacg
     2281 atggacgcca tcgtcggcga gaccgttcgc gcgctcccga ccggcgcggc cctgatggtc
     2341 ctttcggacc acggcttcgc cacctggcgg cgctcggtca actacgactc gtggctcgtc
     2401 gaaaagggct atctcgtgtt gaagggggac gcgcggcggc agaacctcga ggcgctcttc
     2461 tcccgcggag cgttctggga agccgtcgac tggtcccgct cgcgcgcgta cgcgatgggt
     2521 ctcggcgacc tgtacgtcaa cctgcggggc cgggagaagc aggggatcgt cgctcccggc
     2581 gcggagtacg aggctctgcg cgaggagctc gcgcgcggac tgctcgagct gacggatccg
     2641 gcgaccgggg agcgcgccgt ctcgcgggtc ttcaagcgcg aagacgtcta ccgccgctac
     2701 gaccctcgac tcattccgga catgatcgtg gcgaaccgcc ccggatatcg cgtgtcctgg
     2761 caggcgtcgc tcggcgtccc gaccgggacc gtcttcgagg acaaccggga cgtatggagc
     2821 ggcgaccact gctccctcga ccccgacctc gtccgcggtg tcttcttcgc gtcgcggccg
     2881 ttttccgcgc cgcgcgtgcc gacaattacc gacgcgacgg ccgctctccg ggcgctcctc
     2941 ggcggaacgc ccgccgaagg ggcgatcgtc tggaaatgaa cgcgatcgct ccgattgtct
     3001 gcgccctgct cctcttcggc cctcccgctg ctcccgtctc ccgtattgcc tctcccgaat
     3061 ccgagcgcca ggcggatctc gcgcgcctgc gttcgcgcat ccagtcgctt cagggtcagc
     3121 tcgaggacag ccgccgccgg caggcgacgc tttccgaaga gctcgcccgt cttgagctcc
     3181 agctcgagat cgcggctcag gagaaggaac tgctggcgcg gctgcgcgaa gactacgcag
     3241 tccgcctcga agagatcgcc gccgaacgca ggtcggcgga agaggcggcc tcgcggagcc
     3301 gccgcgatct gctcgcccgc gcgcggcttc tccagcgctt cggccgtttc gggtacgtgc
     3361 gcgtgctcct ggaggcgcgg gacgtcccgg ctttcctgga gagtgtctcc cggctcgact
     3421 cgctcgcccg ccgcgatgcc cggctcctca cggagcatcg cgaagcgcag aggcgcctgg
     3481 aatccgggct cgagagggag agggctttga agaccgagac ggaccgtctc tacgccgaga
     3541 gccgtcgcga ggaaagccgc atcgcttcgt tgaagacgga gcgcgagaac ctgctcgaac
     3601 ggcagcgcgc cgtctcggcc tcgcagcgcc tggaggttgc ggcactctcc gacaaggcc
//
LOCUS       DASWAV010000602         3030 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U2665
            k141_275158, whole genome shotgun sequence.
ACCESSION   DASWAV010000602 DASWAV010000000
VERSION     DASWAV010000602.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960316
            Sequence Read Archive: SRR10260007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 3030)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3030)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:47:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,929
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3030
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_275158"
                     /isolate="SMAG_U2665"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.36444 N 132.31889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..197
                     /locus_tag="VF958_12460"
     CDS             <1..197
                     /locus_tag="VF958_12460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9149966.1"
                     /translation="VAAYPIIERIWLHDYLSNEVIRDHAALERAAESGSDSAVHAVLR
                     GQRHADEITRETGVLPSNRR"
     gene            203..685
                     /locus_tag="VF958_12465"
     CDS             203..685
                     /locus_tag="VF958_12465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9149967.1"
                     /translation="MVRRLSLVLAALFFSHFLSAEIVTQKFNWSPVNGVQRLAFDWND
                     IAISEIRFDLGDTIKPLKTSSAKAVVRVDNNSARDQEVGIAIALFDGDGTMLAAGSGG
                     IKVGDLDRGERDTFTVRFPYVYRNIKNTKYFFLTVETKPTGKWKYQPKTRPTEPPPGQ
                     "
     gene            complement(606..1715)
                     /locus_tag="VF958_12470"
     CDS             complement(606..1715)
                     /locus_tag="VF958_12470"
                     /inference="COORDINATES: protein motif:HMM:NF018805.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin family protein"
                     /protein_id="HEX9149968.1"
                     /translation="MRGLFAFALALSIAPRATPAPGGIAFPRDHGAHADAAVEWWYWT
                     GHLSDRAGRAYGFQLTFFRVGELHLAHFAWTDVARREFRYEEKTHLGLPGIASAAEGK
                     LDLANEDWNAQETTGGVQVVAASGRAGELRLTLTPVKTPVLQGDKGLSRKGPGAREFS
                     HYVSITRLAAKGTIRRGSSSEPLAGTAWFDHEWGPGVLPADAAGWDWFALQLDDGSEL
                     MLYRIRRAGGGATPFSSGTFVPARAPSTPIRWSDVRLAEIRTWRSPRTKARYPAGWRI
                     AVASLGLDVTVEPLVADQELETPGSTGVTYWEGACAVKGARRGQPVSGRAYVELTGYA
                     GRDVPGMARLTAPAEARSVSSWAGTSTSRSASSRP"
     gene            complement(1718..>3030)
                     /locus_tag="VF958_12475"
     CDS             complement(1718..>3030)
                     /locus_tag="VF958_12475"
                     /inference="COORDINATES: protein motif:HMM:NF014717.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX9149969.1"
                     /translation="AALSRLGPIGGFPWFGFAAVAFVVVALALVAPALVRGGAAAARP
                     SLERIFGAPGRLASGLFGGSPTRNGIAVTALAMALGMTLAMITTVASMRATVGAWVGS
                     TLRADLWVRAGSGTAAAIGDLPPEIVEFLGSVPGVGAVDPFRVRAGFDAAGRAISIAS
                     SDFRVLSRAGGAPLLDGRDSRAVAQKARRDREVLVSEPYARRYGVARGDSVSLRTPKG
                     IRSFRVAGIYRDFSNDQGTVLLDRSLSLEVFGDPRVTSAGIVAAPGVDAAALRRVILA
                     RAADRFSVDVITTGELRSQVLRIFDRTFAVTNALEAIAVAVAVAGIANALIASAVERR
                     RSFGLLRAIGASPAQIRTAVLLEALLAGIVATAAAFAAAAAFAALLLGVINPQSFGWS
                     VVPQAPPGRLAAAAAVVLAASVLAGIVPGRIAASADPASALAEE"
BASE COUNT          513 a         1052 c         1071 g          394 t
ORIGIN      
        1 tcgtcgccgc ctatcccatc atcgaacgaa tctggctcca cgattacctt tcgaacgagg
       61 tgatccgcga tcacgcggct ctcgaacgcg ccgcggagtc ggggagcgat tcggcggtgc
      121 atgcggttct ccggggacag aggcacgcgg acgagatcac gcgcgagacc ggcgtgttac
      181 cctcgaatcg gaggtaacag gcatggttcg tcggctctcc ctcgtcctcg ccgcactctt
      241 cttctcacat tttctctcgg ccgaaatcgt cacgcagaag ttcaactggt ctccggtcaa
      301 cggagtccag aggctcgcct tcgactggaa cgacatcgcg atctcggaga tccgattcga
      361 cctgggcgac acgatcaagc cgctgaagac ctcctcggcg aaagcggtcg tccgcgtgga
      421 caacaactcg gcgcgcgatc aggaggtcgg gatcgccatt gccctcttcg acggggacgg
      481 gacgatgctc gcggcaggca gcggcggaat caaagtcggc gacctcgacc gcggcgagag
      541 ggacactttc acggtccgct tcccgtacgt gtaccgcaac atcaagaaca cgaagtactt
      601 cttcctcacg gtcgagacga agccgaccgg gaagtggaag taccagccca agacgagacc
      661 gaccgagcct ccgccggggc agtgagccgc gccattccgg gcacgtcgcg tccggcgtac
      721 cccgtcagct cgacgtaggc gcgcccggag acgggctgtc cgcggcgagc gcctttcacg
      781 gcgcacgcgc cctcccagta cgtcacgccg gtcgagccgg gcgtctcgag ctcctggtcg
      841 gcgaccagcg gctcgaccgt aacgtcgagg ccgagagacg ccaccgcgat ccgccacccg
      901 gcggggtacc gcgccttcgt ccgcggcgac cgccaggtgc gtatctccgc cagacggacg
      961 tcgctccagc ggatcggagt cgagggggcg cgcgcgggaa cgaaagtccc ggacgagaag
     1021 ggcgtcgcgc cgccgcccgc gcgtcggatc cggtagagca tcagctccga gccatcgtcg
     1081 agctgcagcg cgaaccagtc ccagccggcg gcgtcggccg gaagcacgcc gggaccccac
     1141 tcgtggtcga accaggcggt tccggcgagc ggctcggagc tcgacccccg gcggatcgtt
     1201 ccctttgccg cgagccgggt gatcgagacg tagtgcgaaa actcgcgtgc tcccggccct
     1261 tttcgagaga gacccttgtc gccctggagc accggggttt tgacgggtgt cagcgtcagt
     1321 cggagctcgc ccgcgcgtcc cgaggcggcg acgacctgga cgccccccgt cgtctcctgc
     1381 gcattccagt cctcgttggc gaggtcgagt tttccttctg cggcggaggc gatgcccggg
     1441 aggccgaggt gcgtcttctc ctcgtagcgg aactcgcgac gcgccacgtc ggtccacgcg
     1501 aagtgggcga ggtgaagctc gcccacgcgg aaaaacgtga gctggaagcc gtacgcgcgg
     1561 ccggcgcggt ccgagaggtg gcccgtccag taccaccact cgacggcggc gtcggcgtgg
     1621 gcgccgtggt cgcggggaaa cgcgattccg ccgggggcgg gcgtcgctcg gggagcgatc
     1681 gagagcgcga gggcgaacgc gaagagacct cgcatcgcta ctcttcggcc agcgcggacg
     1741 cggggtccgc cgaagcggcg attctgccgg gtacgatccc ggcaaggacc gaggcggcga
     1801 gcacgaccgc tgccgcggcg gccaggcgac cggggggcgc ctgcggcacg acgctccagc
     1861 cgaacgactg cgggttgatc acgccgagga gcagcgccgc gaaggctgct gccgcggcga
     1921 acgcggccgc cgttgcgacg atacccgcaa gaagcgcttc gaggagaaca gccgtccgga
     1981 tctgtgcggg cgacgcgccg atcgcgcgaa gaagcccgaa ggagcgcctc cgctccacgg
     2041 ccgacgcgat gagcgcgttc gcgatccccg cgacggcgac ggcgaccgcg atcgcctcga
     2101 gcgcgttcgt caccgcgaac gttcggtcga agatccgcag gacctggctg cgcagctcgc
     2161 ccgtcgtgat cacgtcgacg gaaaagcgat ctgccgcccg cgcgagaatc acccgccgga
     2221 gcgctgccgc gtccacgccc ggcgccgcga cgatgcccgc gctcgtcacg cgcggatcgc
     2281 cgaagacctc aagcgagagg gaccgatcga ggagcaccgt cccctggtcg ttcgagaaat
     2341 cacggtagat gccggcgacc cggaacgagc gaatgccctt cggcgtgcgg agcgagacgc
     2401 tgtcgccacg ggccaccccg tatcgccgag cgtacggctc cgagacgagg acctcgcggt
     2461 cgcggcgtgc tttttgcgcg accgctcgtg agtcgcgtcc gtcgagaagg ggcgctccac
     2521 ccgcgcggga gaggacgcgg aagtcgctcg acgcgatgct gatcgcgcgg ccggcggcgt
     2581 cgaagcccgc gcgtacgcgg aacggatcga cggcgcccac gcccggcacc gacccgagga
     2641 actcgacgat ctcgggaggc agatcgccga tcgcggccgc ggtccccgaa cctgcgcgga
     2701 cccagaggtc cgcgcggagg gtcgaaccga cccatgctcc tacggtcgcg cgcatggagg
     2761 cgacggtcgt gatcatggcc agggtcatcc cgagggccat tgcgagcgcg gtgacggcga
     2821 tgccgtttcg ggtgggcgag ccaccgaaga gacccgacgc gaggcgcccc ggtgcgccga
     2881 aaatcctctc gagcgaagga cgcgcggccg cggccccgcc gcgcacgaga gccggcgcga
     2941 cgagcgcgag cgccacgacg acgaaagcga cggcggcgaa cccgaaccac gggaacccgc
     3001 cgatcggtcc aaggcgggag agcgcggctg
//
LOCUS       DASWAV010000603         3696 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U2665
            k141_515965, whole genome shotgun sequence.
ACCESSION   DASWAV010000603 DASWAV010000000
VERSION     DASWAV010000603.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960316
            Sequence Read Archive: SRR10260007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 3696)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3696)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:47:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,929
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3696
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_515965"
                     /isolate="SMAG_U2665"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.36444 N 132.31889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..297
                     /locus_tag="VF958_12480"
     CDS             <1..297
                     /locus_tag="VF958_12480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9149970.1"
                     /translation="KAAARAFPTAFHGRKYSVKSSTALRAFIRIVPYVISMIRKRGGD
                     VWSAGELDAALRPWSESIGDRRFETDTVWREKMTAGKRSAVEILARELRQGLNA"
     gene            367..948
                     /locus_tag="VF958_12485"
     CDS             367..948
                     /locus_tag="VF958_12485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012003125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HEX9149971.1"
                     /translation="MGAIGGCLLFLILIAVFAAIWAIWRYNNLVALKYQVANAWKQID
                     VQLKRRHDLIPNLIAAVRGEMQFEQDTLEKVIAARNSAVAARGVAETAEKENALTQTL
                     SRLFALAENYPNLKANESVRSLMEELTSTENKVGFARQFYNDVATRFNTAQNVFPDSL
                     IAQTFKFQPVELFEITEPAEREVPKVDLNIRPQ"
     gene            971..1927
                     /locus_tag="VF958_12490"
     CDS             971..1927
                     /locus_tag="VF958_12490"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013594.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metalloprotease"
                     /protein_id="HEX9149972.1"
                     /translation="MTGASLFEQQKKNLRRTTGILAGFVAFLVFLGAGADLFLYGSGA
                     GPAFPVATIGALLFGAGSSLWALKAGDRAVLESANAVPVDPRDPRQRVLDNVVEEMAI
                     AGGLPKPAVYVIPDGDPNAFATGSGPEKSSVAVTTGLLDALNREELQGVVSHEMAHVK
                     NYDIRLMTVVAALVGSVLLISDWGRRGIGWGGGRRRGSRGGGIFALVFFVLWIVSLLL
                     APLIARLVAMAVSREREYLADASGSELTRNPLALASALEKIDAAVAPTPSIKQGVAHL
                     CIADPRGREINEREGGLAELFATHPPIAKRIALLREMSYQPR"
     gene            complement(1915..2436)
                     /locus_tag="VF958_12495"
     CDS             complement(1915..2436)
                     /locus_tag="VF958_12495"
                     /inference="COORDINATES: protein motif:HMM:NF015597.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LOG family protein"
                     /protein_id="HEX9149973.1"
                     /translation="MTLSIAVFGSSRVPQDSPDYEHAFVLGREIAQRGARVVCGGGGG
                     VMEAVCRGAAEAGGRTLGVVIGDAAPNAWVTDLLRERDLAARLRRLRDESQAGMFLPH
                     GLGTMLEIVWMAESIAKGQVAPRPLVFLGLFWRRTVALAVSEAVGPGAGALVESIRFA
                     SAPEEAVAAVYRG"
     gene            complement(2433..2720)
                     /locus_tag="VF958_12500"
     CDS             complement(2433..2720)
                     /locus_tag="VF958_12500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9149974.1"
                     /translation="MDFELLCPCCGAKLKVDPELKAIIAHEAPKVARKFESVDDAIEG
                     LKTHDAEREKKFAASVEAEKSKRDVLNRKFEELFEKAKTDTSKPIRDIDLD"
     gene            complement(2737..3141)
                     /locus_tag="VF958_12505"
     CDS             complement(2737..3141)
                     /locus_tag="VF958_12505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006594455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HEX9149975.1"
                     /translation="MTGSSEPSAPGSVRLDVWLDVACVFPTRSQAKAACEGGKVDVNG
                     ARAKPHRLVRAGDRLEVTTGPSRRRSLVVRGVAERSIPKAAARALYDDVTPPPTAEEI
                     EVRKMERFFAPSASAGRPQRRERRELRRRKGW"
     gene            complement(3158..>3696)
                     /locus_tag="VF958_12510"
     CDS             complement(3158..>3696)
                     /locus_tag="VF958_12510"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HEX9149976.1"
                     /translation="PEGLDALWRALDEFAFAGSRLVLDLVEVENVASLDALVRAVGEA
                     RQHGAAVALDDVTSPYRAIQLCEALRPEWVKVDCDITRGVARDVRRRPILKVFGRLAR
                     QFAFGLIAEGVETASDLDACRAAGVVAAQGYFIGRPAADPPAAEPAFLEWVARRRTSP
                     QEDAEENGENHETEERAS"
BASE COUNT          609 a         1252 c         1237 g          598 t
ORIGIN      
        1 aaggcggctg ctcgcgcttt tccgaccgcg ttccacggac gtaagtactc ggtcaagagt
       61 tcgaccgcgc tgcgcgcctt cattcgaatc gtgccgtacg ttatttccat gattcgcaaa
      121 cgtggcggcg acgtctggtc cgccggggag ctcgacgccg cgctccgtcc gtggtccgag
      181 tcgatcggcg acaggcgctt cgaaaccgac acggtctggc gcgagaagat gaccgctggc
      241 aagcgctccg ccgtcgagat tctcgcgcgc gagctccggc aggggctcaa tgcctagcga
      301 aatgcgggga ggggaggcgt ggagtcaaac ctcggcgcgg cggaatcgta gatagaggta
      361 caagctatgg gagccatcgg cggttgtctt ctctttctca tcctgatcgc ggtcttcgcc
      421 gcgatctggg ccatctggcg ctacaacaac ctggtcgcgt tgaagtacca ggtcgcgaac
      481 gcctggaagc agatcgacgt gcagctgaaa cggcggcacg acctcattcc gaacctgatc
      541 gcggcggtcc ggggcgagat gcagttcgag caggacactc tcgagaaagt catcgccgcg
      601 cgcaacagcg ccgtcgcggc tcgcggggtg gcggagaccg ccgagaagga gaatgctctc
      661 acgcagaccc tctcgcgcct cttcgcgctc gccgagaact acccgaacct gaaggccaac
      721 gagagcgtcc gttccctcat ggaggagctg acgtcgaccg agaacaaggt cgggttcgcg
      781 cgtcagttct acaacgacgt cgcgactcgg ttcaacacgg cgcagaacgt cttcccggac
      841 agcttgatcg cgcagacgtt caagttccag ccggtggagc tcttcgagat cacggagccg
      901 gcggagcgcg aggtcccgaa ggtggacctc aacatccgcc cgcagtagct cgccccgagg
      961 acggcccgct ttgaccggcg ccagcctctt cgagcagcag aagaagaacc tccggcgcac
     1021 gacggggatc ctcgcgggct tcgtcgcttt cctcgtgttt ctgggggccg gcgccgacct
     1081 ctttctttac gggtccggcg cgggtcccgc gtttccagtt gccaccatcg gcgctctcct
     1141 tttcggcgcg gggtcgtccc tctgggcgct caaggcgggc gatcgagccg tgctcgaatc
     1201 cgcgaacgcc gtgcccgtcg atccgaggga tccacggcaa cgcgtgctgg acaacgtcgt
     1261 cgaagagatg gcgatcgcgg gcggacttcc gaagccggcg gtctacgtca tcccggacgg
     1321 cgacccgaac gcgttcgcca ccgggagcgg accggagaag tcatcggtcg cggtgacgac
     1381 cggtctcctg gacgcgctga accgcgagga gctccagggc gtggtctccc atgagatggc
     1441 gcacgtgaag aactacgaca tccggctgat gaccgtggtc gcggcgctcg tcgggtcggt
     1501 cctgctgatc tccgactggg gccggcgggg gatcggctgg ggaggcggac ggcggcgcgg
     1561 gagccgcggc ggcggcattt tcgccctggt tttctttgtc ctctggatcg tgtcgctcct
     1621 tctcgcgccg ctgatcgcgc gcctcgtcgc aatggcggtc tcccgcgagc gcgaatacct
     1681 cgcggatgcc tccggctcgg agctcacgag gaacccgctg gcgctcgcgt cggcgctcga
     1741 gaagatcgac gcggcagtcg ctccgacgcc ctcgatcaag cagggagtcg cccacctctg
     1801 tatcgccgat ccccgcggac gcgagatcaa cgaacgcgag ggaggtctgg cagagctgtt
     1861 cgccacccat ccgccgatcg caaaacgaat cgcgctcctg cgcgaaatgt cgtatcagcc
     1921 gcggtagacc gctgcgaccg cctcttccgg cgcggaggcg aaacgaatgc tttcgacgag
     1981 cgctcccgcg ccggggccga ccgcttcgga cactgcaaga gcgaccgttc ggcgccagaa
     2041 gagaccgagg aagaccagcg gtcgaggcgc cacctggccc ttggcgatcg actccgccat
     2101 ccacacgatc tcgagcatcg tgccgagacc gtgcggaagg aacatgcccg cctgggactc
     2161 gtcgcgcaga cggcgcagcc tcgcagcgag atctcgctca cgaaggagat ccgtcaccca
     2221 ggcgttcgga gccgcgtcgc cgatcacgac tccgagggtg cggccccccg cctccgccgc
     2281 gccccggcag acggcttcca tgacaccgcc gccgccgccg cagacgaccc gcgcgccgcg
     2341 ttgcgcgatc tcccggccga ggacgaaagc gtgctcgtag tcggggctgt cttgcgggac
     2401 gcggctcgaa ccgaagaccg cgatcgagag cgtcaatcca gatcgatgtc ccggatcggt
     2461 ttcgaggtgt ccgtcttcgc tttttcgaac agctcctcga acttccggtt caggacgtcg
     2521 cgcttgctct tttccgcttc gacggaagcc gcgaacttct tctcgcgttc ggcgtcgtgg
     2581 gtcttcagtc cctcgatcgc gtcgtcgacc gactcgaact tgcgcgcgac cttcggcgcc
     2641 tcgtgcgcga tgatcgcctt cagctccggg tcgaccttga gtttcgcgcc gcaacagggg
     2701 cagaggagct cgaaatccac gacaggagtc taccgctcac cagcccttcc ggcggcggag
     2761 ctcgcgtctc tcccggcgct gtggccgtcc agcggacgcc gacggcgcga aaaagcgttc
     2821 catcttccgc acctcgatct cctcggcagt cggcggcggc gtcacgtcgt cgtagagagc
     2881 gcgcgcggcc gctttcggga tcgagcgctc ggcgactccg cgcacgacga gggagcggcg
     2941 gcgcgaagga cccgtcgtca cttcgagtcg gtcgccggcg cgcacgagcc ggtggggctt
     3001 ggcgcgggcg ccgttgacgt ccacttttcc cccctcgcag gcggctttcg cctgagaccg
     3061 ggtcggaaaa acacaggcga cgtcgagcca gacgtcgaga cggaccgagc cgggcgcgga
     3121 aggctccgaa ctcccggtca cgacagggcc gacgcgccta cgaagcgcgt tcctccgtct
     3181 cgtggttctc gccgttctct tcggcgtctt cctgcggaga ggtccgtcgg cgcgcgaccc
     3241 attcgagaaa ggcgggttcg gcggcagggg gatcggcggc aggacggccg atgaagtagc
     3301 cctgcgcggc gacgactccg gcggcacgac aggcgtcgag atcggaggcg gtctccaccc
     3361 cctcggcgat gagaccgaag gcgaactgac gggccagccg gccgaatacc ttcaggatgg
     3421 ggcggcggcg cacgtctcgt gcgacccccc gggtgatgtc gcaatccact ttcacccact
     3481 ccggccggag cgcctcgcag agctgaatcg cgcgatacgg agaggtcacg tcatcgagcg
     3541 cgacggcagc gccgtgctgc cgagcttctc cgacggcgcg gacgagggca tcgagcgacg
     3601 caacattctc cacctcgacg aggtcgagca cgagccgcga cccggcgaag gcaaattcat
     3661 cgagagcgcg ccagagggcg tcgagccctt ccgggg
//
LOCUS       DASWAV010000604         4491 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U2665
            k141_555396, whole genome shotgun sequence.
ACCESSION   DASWAV010000604 DASWAV010000000
VERSION     DASWAV010000604.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960316
            Sequence Read Archive: SRR10260007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 4491)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4491)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:47:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,929
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4491
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_555396"
                     /isolate="SMAG_U2665"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.36444 N 132.31889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..707
                     /locus_tag="VF958_12515"
     CDS             <1..707
                     /locus_tag="VF958_12515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9149977.1"
                     /translation="ATERVRRAADEARNATRAARGAAERARAPEAASALYESARRKEK
                     EGQKSLGAGRYETALASFESATALFRQAETWSRSAPVRPAERIAVIPPTREPAPEPTR
                     PAPTAAIAVSEPRPTPAKPTEPKTRSEEERVREAVGLYVEAQNSLDVALYSRVYPGLA
                     GERRQMVEQAFGNLKSQTLELDIQRIEITGSRAQVVGYERRLAVPRVGSEQRDARQRM
                     IHLEKRGDAWVITELR"
     gene            complement(726..2057)
                     /locus_tag="VF958_12520"
     CDS             complement(726..2057)
                     /locus_tag="VF958_12520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9149978.1"
                     /translation="MPLRRLTTLALALAASTAPILGQAFNLRDLLTEFLREGITLAPP
                     ASGPSHVAHFIGQDSPQFLALGQFSAAVANQLSSFPIASSAGGFTYRFDPALGVFTRT
                     SDSFGPVYAERADTIGKGKFNIGLNYSHYTFDRIDDLSLRDGDVRLVFTHQDVNNDNS
                     NLEPFVEGDVITASLFIKIETDITALVVSYGVSDRLDLGLAVPLVRVDLEAQTDASIQ
                     RLATGAVAPDIHRFLNGGTTETFRQSGTASGVGDVVLRGKFRLLRGERGGLSIAEDLR
                     LPTGEERDLLGTGGTQAKTFVIGSLHLGSFSPHVNGGYTWSVGSGDAGEIPDEISYTA
                     GFDWAVSPRMTVAVDVLGRDFLSSRSVQVVNTTFQANTNTDPTAPPTIVSATFPRLVA
                     EQGDSNSVLGSIGLKINPFGNFLLTVNGLFRLNKRGLQDDFSPLVAVDYSF"
     gene            complement(2087..3046)
                     /locus_tag="VF958_12525"
     CDS             complement(2087..3046)
                     /locus_tag="VF958_12525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012241400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEX9149979.1"
                     /translation="MKSARRRESAGPPTRLPAYDAIIVGGGPAGLSAALILGRCRRRV
                     LLCDAGRPRNAVSRALHGFLTRDGIRPGDLLAIGRRQLAKYGVEFLAAEVTDARRRAG
                     GFEVRITGGRRLFARKLLFATGVVDRLPEVDGVRALYGESVFHCPYCDGWEVSDRPLA
                     VYGRGRSGMGLSLSLRTWSREVALVSNGPVRLDRRERRRLASQDIEIVENRIVRLDRR
                     RKGLDIVFAGGERLARAALFFSTGQDPACDLPRRMGCRFTRKGAVRTNRLEGTGVPGL
                     YVAGDASRDVQLAVIAAAEGAKAAFAMNVAMQEEEGLGARQQR"
     gene            complement(3043..3768)
                     /locus_tag="VF958_12530"
     CDS             complement(3043..3768)
                     /locus_tag="VF958_12530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9149980.1"
                     /translation="MLREARRLLPPRAVLFGVFLATAPFGLVSTLPALQGGGTSSPAA
                     VLVVVPPGSKLAVVERTRSAVHAPRRALLVADSDRVVLDGGFEMLVDRSFARAPDAEV
                     IVLAEGEPGRAEEAFLTERRKTAKAILLPRGSRLAEKLREGGSGGALILLGGSESIAP
                     LLDAFGESGSARANASSTSEVPVAGPAPTAIPPTPAASGHTATSPAAAAENPPARTPT
                     PATARVFDRYFSSSRPTPTPTPR"
     gene            4032..>4491
                     /locus_tag="VF958_12535"
     CDS             4032..>4491
                     /locus_tag="VF958_12535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002611596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HEX9149981.1"
                     /translation="MPGAPAAPAAIERDWSDDVLYFVLVDRFADGDLSNDRHVDRTAK
                     GAFHGGDLKGLTENLDEIAGLGVTAIWVNPLVKNIDGYVTGAGFPDWAYHGYWADDFT
                     RLDERFGTEEDLKALVAACHRRRIKVLLDVVYNHAGYGSHYLSDPATKAWL"
BASE COUNT          759 a         1510 c         1566 g          656 t
ORIGIN      
        1 aagcgacgga gagagttcgg cgggccgcgg atgaggcccg gaacgcgacg cgagccgcgc
       61 gcggcgccgc cgagcgcgcg agggcaccgg aggccgcctc ggcgctctac gaatccgcgc
      121 ggcgcaagga aaaagaggga cagaagagcc tcggggcagg ccggtacgag acggcgctcg
      181 catccttcga atcggcgacg gcgctgtttc ggcaggcgga gacctggtcc cgctcggcgc
      241 cggtacgccc ggccgaacgg atcgcggtca tcccgccgac ccgcgaaccg gctccggagc
      301 cgacgcgacc tgcacccacg gcggcgatcg cggtctcaga gccgcgcccg acaccagcga
      361 agccgaccga accgaagaca cgttcggagg aggagcgcgt acgagaggcc gttggtctct
      421 acgtcgaggc gcagaactcg ctcgacgtcg ccctgtattc gcgggtctat ccggggctcg
      481 cgggcgagcg acggcagatg gtcgagcagg ccttcgggaa cctcaagtcc cagacgctgg
      541 agctcgacat tcaacgaatc gagatcaccg gctcacgcgc gcaggtcgtc ggatacgagc
      601 ggcgcctcgc ggtcccccgc gtcggaagcg agcagcgcga cgcacgtcag cggatgatcc
      661 acctggagaa gcgtggcgac gcgtgggtga tcaccgagct gcgctaggag agtcggaaat
      721 ccgtcctaaa agctgtagtc caccgcgacc agcggtgaga aatcgtcctg caaccccctc
      781 ttgttcagac gaaagaggcc attcaccgtc agcaggaagt ttccgaacgg attgatcttc
      841 aatccaatcg agccgaggac cgagttggag tcgccctgtt cggcgacgag ccgcgggaac
      901 gttgcggaga cgatcgtcgg aggcgctgtc ggatcggtat tcgtgttcgc ctggaaggtc
      961 gtgttgacga cctgcacgct tcgcgagctc agaaagtctc ttccgagcac gtccaccgcg
     1021 acggtcatcc gcgggctcac cgcccagtcg aacccggccg tgtaggagat ctcgtcgggg
     1081 atctcccccg cgtcaccgga cccaaccgac caggtgtaac cgccgttgac gtgcggcgag
     1141 aaggagccga ggtggagcga gccgatcacg aacgtttttg cctgtgttcc gccggtgccg
     1201 agaaggtcgc gctcctctcc ggtcggcagc cgcaggtcct cggcgatcga aaggccgccc
     1261 cgttcgcctc tgagaagtcg gaacttcccc cgcagaacga cgtcgccgac gccgctcgcg
     1321 gttccgctct gccggaacgt ctccgtcgta ccgccgttca ggaaccggtg gatatccggg
     1381 gccaccgcgc ccgtcgcgag cctctggatg gacgcgtctg tctgtgcctc gagatcgaca
     1441 cggacgagtg ggacggccag gccgaggtcg aggcggtccg agaccccgta actcacgacg
     1501 agggccgtga tgtccgtctc gatcttgatg aacagcgacg cggtgatcac gtccccctca
     1561 acgaacggct cgaggttgct gttgtcgttg ttgacgtcct ggtgcgtgaa gacaagcctc
     1621 acgtctccgt cgcgcagtga gaggtcgtcg attcgatcga acgtgtagtg gctgtagttg
     1681 agtccgatgt tgaacttgcc ctttccgatc gtgtcggcgc gctccgcgta taccgggccg
     1741 aagctgtcgg atgtgcgcgt gaagacaccg agggcgggat cgaaccggta cgtgaagccg
     1801 cccgccgacg aggcgatcgg gaaggacgag agctgattgg cgacggccgc gctgaactgg
     1861 ccgagcgcga ggaactgtgg cgagtcctga ccgatgaagt gggcgacgtg gctcggaccg
     1921 ctcgccgggg gagcgagagt gatcccctcg cgcaggaact cggtcaggag gtcgcggagg
     1981 ttgaaggcct gaccgaggat cggcgccgtc gaagcggcca gcgcgagagc gagcgtcgtc
     2041 aggcggcgaa gcggcatgga acctccgttc gggactgagc gcgattctac ctttgctgtc
     2101 tggctccgag cccttcttcc tcctgcatcg cgacgttcat cgcgaacgcg gcctttgcac
     2161 cctcggccgc agcgatgacg gcgagctgaa cgtcccgcga tgcatctccc gcgacgtaga
     2221 ggcccgggac tcccgtcccc tcgagacggt tcgttcggac cgcccccttg cgcgtgaaac
     2281 ggcaacccat ccggcgcggg agatcgcacg cggggtcctg cccggtcgag aagaagagag
     2341 ccgctcgagc gagacgctcg ccgccggcga agacgatgtc gagacccttt cggcgacgat
     2401 cgagccggac gattcggttc tcgacgatct cgatgtcctg cgaggcgagt cggcggcgct
     2461 cccgtcggtc gaggcgcacc gggccgttgg acacgagcgc gacctcgcgg ctccaggtcc
     2521 ggagcgagag cgagagaccc atccccgacc gtccgcgccc gtagaccgcg agcggtcggt
     2581 ccgagacctc ccaaccgtcg cagtacggac agtgaaagac gctctcgccg tagagcgcgc
     2641 ggactccatc gacttcgggg agacgatcga cgaccccggt cgcgaagagc agcttgcggg
     2701 cgaagaggcg gcggccgccg gtgatccgga cctcgaagcc gcccgccctc cggcgcgcat
     2761 ccgtgacctc ggccgcgagg aactcgactc cgtacttcgc gagctgccgg cggccgatgg
     2821 caaggaggtc gccggggcgg attccgtcgc gggtgagaaa gccgtgcagc gcccgggaga
     2881 cggcgtttcg cgggcggccc gcgtcgcaca ggagaacccg gcggcgacac cgcccgagga
     2941 tgagcgccgc cgagaggccc gccggtcccc cgccgacgat gatcgcgtcg tacgccggga
     3001 gacgcgtcgg cggcccggcc gactcacgcc tgcgggcgct cttcaacgcg gggtgggagt
     3061 cggcgtcggc cgtgacgagg agaagtaccg gtcgaacacg cgcgcggtcg ccggcgtcgg
     3121 cgtgcgagcg ggaggattct ccgcggcggc agccggcgag gtcgcagtat ggccgctcgc
     3181 cgccggggtc ggaggaatcg ccgtcggcgc cggtccggcg acggggacct cgctcgtgga
     3241 cgaggcgttg gcgcgggccg aaccgctctc tccgaatgcg tcgagcagcg gcgcgatcga
     3301 ctcgctcccg cccagcagga tgagcgcgcc tcccgatcct ccttcgcgga gcttctccgc
     3361 gaggcgcgag ccgcgcggga gcaggatggc cttcgcggtc tttcttcgct ccgtgaggaa
     3421 cgcttcctcg gcgcgcccgg gctccccctc tgcgaggacg atcacttccg cgtcgggcgc
     3481 ccgtgcaaag gaacgatcga ccagcatttc gaagcccccg tcgaggacga cgcgatcgga
     3541 gtcagcgacg agcagggcgc gcctcggggc gtgcacggcc gaccgggtcc tctcgaccac
     3601 cgcgagcttc gaccccggcg ggacgaccac gaggacggcc gccggggacg acgtgccgcc
     3661 gccctgaagg gcggggagag tcgagacaag cccgaacggc gcagtcgcga ggaagacccc
     3721 gaaaaggacg gctcgggggg ggaggaggcg ccgcgcttct cgcaacatcg acggggcgca
     3781 agcgtaacag cactgtcgct gtcgcgggga cacccgccgc atttcacaca cttgattgac
     3841 cttccgcggt cacccgaaat agagtcgaac ggtcgttgcc gtctcaaaaa tccggtcccg
     3901 ccgccgctcg tccggaatgt cccgacgtac ggcgtgtgcc cgtcctcctg cctctcttgc
     3961 tcctttcaat ctctttcctc cgttgcgctc cccccgccac tccgaaaagg ccggcagccg
     4021 cgtctgcagc ggtgccggga gcgcccgccg cgccggccgc gatcgagcgc gactggtcgg
     4081 acgacgttct ctacttcgtt ctcgtcgacc ggttcgccga cggcgacctc tcgaatgacc
     4141 gccacgtcga tcggacggcg aaaggagcgt tccacggcgg ggacctgaag ggcctgacgg
     4201 aaaacctgga cgagatcgcg ggcctcggcg tcaccgcgat ctgggtgaat ccgctcgtca
     4261 agaacatcga cggctacgtc accggcgccg gatttccgga ctgggcgtac cacgggtact
     4321 gggcggacga cttcacccga ctcgacgaac ggttcgggac cgaggaggac ctgaaggcgc
     4381 tcgtcgccgc gtgccaccgg aggcggatca aggtcctgct cgacgtcgtc tacaaccacg
     4441 cggggtacgg ctcgcactac ctctcggacc ccgccacgaa agcgtggctt c
//
LOCUS       DASWAV010000605         4697 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U2665
            k141_754165, whole genome shotgun sequence.
ACCESSION   DASWAV010000605 DASWAV010000000
VERSION     DASWAV010000605.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960316
            Sequence Read Archive: SRR10260007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 4697)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4697)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:47:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,929
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4697
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_754165"
                     /isolate="SMAG_U2665"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.36444 N 132.31889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..3467
                     /locus_tag="VF958_12540"
     CDS             <1..3467
                     /locus_tag="VF958_12540"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEX9149982.1"
                     /translation="ARPLAGFIARLFGIEREWRAQASSAGPEAALFRFRRDFLLRRAA
                     RTAAAPDDGLPALMRDARAIEHELFGELPWSEDPELSTARMASELLDLEAEHIEAVRQ
                     KKRPSVSDEARRRTADLAARAATATADLPRPRDDSDEAAMEFLEALLGRYASWCRERL
                     HRPELRRDVAGWVSFRLPEPFDFHRLVATQRPRPDFPEERVGPESRRRRRDGFRLTDP
                     RMTRREVLGETHYCILCHERDKDSCAKGFLDAKTGTWQKNPLGIELAGCPLDEKISEM
                     HQLRREGDSIGALALVAIDNPMCPGTGHRICNDCMKGCIFQKQDPVNIPQIETGVLTD
                     VLSLPWGVEVYGLLTRWNPLSPLRPYPLAYNGKKILVVGLGPAGYTLAHYLLNEGFGV
                     VGIDGLKIEPLPADWTGADGLGIRPIRDCASISRPLDERPLAGFGGVSEYGITVRWDK
                     NFLTLVHLTLARRQRFSILGGVRFGGTITAEQAFELGFDHVAIAAGAGRPTIIAMKNN
                     ILRGVRKASDFLMALQLTGAFKEEALANLQAELPAVVIGGGLTAIDTATELLAYYPIQ
                     AEKTLARYEALVAERGEEAVRAVFDAEERETLDRLLRHGGAVRRERARALAVGEKPDF
                     ARLCREWGGVSIVYRRSMEESPAYRLNHEEIIKALEEGISFVEGLEPAEAVPDEWGKL
                     GSVKFRRSGSGELVELPARICLVAAGTTPNITYAKEFPDALPLDERRRFFAPHRAVRG
                     GDGAWRLDPAPPTDEGAFFTGYSRGGKLISFFGDNHPAYNGNVVKAMASARNGYRAIA
                     AVLDDHRPASAEPDKEREAEWEAFRSVIEERFVARVVEVRRLTPTIVEVVVRAPAAAQ
                     NFEPGQFFRLQNLEKYAPSIDGSPLLIEPLALTGAWVDAGHGLLSMVALELGASSRIC
                     AALKPGEPVLAMGPTGAATELPENGTVLLAGGGLGNAVLFSIGKKARARNNRVIYFAG
                     YKKAEDFYKREELEAASDVLVLSVDRGEPIPTRRREDRQFVGNIVEAMTAWAEGRLGD
                     TPIRLAEVDRLIAIGSDRMMAAVARARHGVLAPHLRPDHVGIGSINSPMQCMMKEVCA
                     QCLQRHVDPATGKEKEIVFSCFNQDQKLDEMDWENLAARLRQNTVSEKLTNLWLERLF
                     SLQDPRLV"
     gene            complement(3508..4248)
                     /locus_tag="VF958_12545"
     CDS             complement(3508..4248)
                     /locus_tag="VF958_12545"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HEX9149983.1"
                     /translation="MLDSVEVLTADGHVLSGTPTTPLEAHNYPPSPCLPEDPPVIEAH
                     IHVADTEILEREDGELLAPRHDSSRISKIARRLGSLFRPKSATARVRPKDVDLSELLA
                     ENFLIPVSGVDRDRLRDSFLESRGQYARHLAIDIGAPRGTPILATDDGEVVRLSRERR
                     GGITIYQKDSSGRYLFFYCHLSRYAGGLSVGQKVSKGDVIGYVGATGHVIGGPHLHFS
                     ITRLPEDDDNLREGLAINPYLLFLAGVP"
BASE COUNT          772 a         1564 c         1629 g          732 t
ORIGIN      
        1 cggcgcggcc gcttgcggga ttcatcgccc ggctcttcgg gatcgagcgc gagtggaggg
       61 cgcaggcctc gtccgcgggt ccggaggcag ccctgttccg tttccggcgc gactttctgc
      121 tgcgccgtgc cgcgcggaca gcagcggcgc cggacgacgg cctgccggcg ctcatgcgag
      181 atgcccgcgc gatcgaacac gagctcttcg gcgagctccc ctggtcggaa gaccccgagc
      241 tttcgaccgc tcggatggcg tcggagctcc tcgacctcga ggccgagcac atcgaggcgg
      301 tccgccagaa gaagcgcccg tcggtctcgg acgaggcgcg ccgccggacc gccgatctcg
      361 ccgcacgcgc cgccaccgcc accgccgatc tcccgcgtcc gcgtgacgac tccgacgagg
      421 ccgccatgga gtttctcgag gcgctactcg gccgttatgc gagctggtgc cgcgagcgcc
      481 ttcatcggcc ggagcttcgc cgcgacgtcg ccgggtgggt ctccttccgc cttcccgagc
      541 cgttcgactt ccaccggctg gtcgcgacgc aacgcccgcg tccggatttc cccgaggaga
      601 gggtggggcc cgagtcccgc cgccgccgga gggacgggtt ccgcctgacg gacccgcgaa
      661 tgacccgccg cgaggtcctc ggcgagaccc actactgcat tctctgccac gagcgggaca
      721 aggactcctg tgcgaagggc ttcctcgacg cgaaaaccgg gacgtggcag aagaacccgc
      781 tcgggatcga gctcgccggc tgcccgctcg acgaaaagat ctccgagatg catcagctcc
      841 ggcgcgaggg ggactcgatc ggcgcgctcg cgctcgtcgc catcgacaac ccgatgtgcc
      901 cgggaaccgg ccaccgcatc tgcaacgact gcatgaaggg atgcatcttc cagaagcagg
      961 acccggtcaa catcccgcag atcgagaccg gcgttctgac ggacgtcctc tcccttccgt
     1021 ggggcgtcga ggtctacggt ctcctcaccc ggtggaaccc gctctcgccc ctgcgcccgt
     1081 atccgctcgc atacaacggc aagaagatcc tcgtcgtcgg gctcggcccg gctggctaca
     1141 ccctcgccca ctacctcctg aacgagggat tcggggtggt cgggatcgac gggctgaaga
     1201 tcgagccgct cccggccgat tggaccggcg ccgacggcct cgggatccgg cccatccgag
     1261 actgtgcatc gatctcgcgc ccgctcgacg agcgcccgct cgcggggttc ggcggcgtct
     1321 ccgagtacgg gatcaccgtt cggtgggaca agaacttcct gacgctcgtg cacttgacgc
     1381 tcgcccggcg gcaacggttc tcgatcctgg ggggcgttcg cttcggcgga acgatcaccg
     1441 ccgagcaggc tttcgagctg gggttcgatc acgtcgcgat cgccgccggc gcgggccggc
     1501 cgacgatcat cgcgatgaag aacaacatcc tgcgcggggt gcgcaaggcc tccgactttc
     1561 tgatggcgct gcagctgacg ggcgcgttca aggaggaggc gctggcgaac ctgcaggccg
     1621 agctccccgc ggtcgtgatc ggcggcgggc tgacggcgat cgacaccgcg acggagctcc
     1681 tcgcgtacta cccgatccag gcggagaaga ccctcgcgag gtacgaggcg ctcgttgccg
     1741 agcgcggcga ggaggcggtt cgggcggtat tcgacgccga ggagcgcgag acgctcgatc
     1801 ggctcctgcg acacggaggg gcggttcgac gcgagcgagc acgggcgctc gcggtcgggg
     1861 aaaagccgga tttcgcgcgt ctctgccgcg agtggggcgg cgtctcgatc gtgtaccgcc
     1921 gctcgatgga ggaatcgccc gcgtatcgcc tgaaccacga ggagatcatc aaggcgctcg
     1981 aggaaggcat ctcgttcgtc gagggtctgg agcccgccga ggcggtgccc gacgaatggg
     2041 gaaagctcgg ctcggtgaag ttccgacgct ccggcagcgg cgaactcgtc gagctcccgg
     2101 cccgcatctg cctcgtcgcg gcgggcacga cgccgaacat cacttacgcg aaggagtttc
     2161 cggacgcgct ccctctcgat gagcgccgcc gctttttcgc gccgcaccgg gcggtgcgcg
     2221 ggggcgacgg tgcctggcgc ctcgatcccg cgcctccgac cgacgaaggg gctttcttca
     2281 cggggtactc gcggggcggc aagctcatca gttttttcgg tgacaaccac cccgcctaca
     2341 acgggaacgt cgtgaaggcc atggcgtccg cgaggaacgg ataccgggcg atcgcggcgg
     2401 tgctcgacga ccaccgacct gcctcggcgg agcccgataa ggagcgggag gccgagtggg
     2461 aagccttccg ctccgtgatc gaggagcggt tcgtcgcgcg cgtcgtcgag gtccgcaggt
     2521 tgacgccgac gatcgtcgaa gtcgtcgtgc gcgcgccggc ggcggcgcag aacttcgagc
     2581 cgggccagtt cttccggctc cagaacctgg agaaatacgc cccgtcgatc gacggctcgc
     2641 cgctcctgat cgaaccgctc gcgttgacgg gcgcctgggt cgacgccggc cacggtctcc
     2701 tttcgatggt cgcgctcgag ctcggcgcct cgtcccggat ctgcgcggcc ctgaagccgg
     2761 gcgaaccggt cctcgcgatg ggtcccacgg gagccgcgac ggagctgccg gagaacggga
     2821 ccgtgcttct cgcgggcggc gggctcggaa acgccgtcct gttctcgatc ggaaagaagg
     2881 cgcgtgctcg gaacaaccgc gtcatctact tcgccggcta caagaaggcg gaagacttct
     2941 acaagcgcga ggagctcgag gccgcctccg acgtcctcgt gctctcggtc gaccgcggcg
     3001 agcccattcc gacgcgccgg cgtgaagacc ggcagttcgt cgggaacatc gtcgaggcga
     3061 tgaccgcgtg ggcggaaggg agactcggcg acacgccgat ccggctcgcc gaggtcgatc
     3121 ggctcattgc gatcgggtcg gatcgcatga tggccgcggt cgcccgcgcc cggcacgggg
     3181 tcctcgctcc ccacttgagg cccgaccacg tgggcatcgg ctcgatcaac tcgccgatgc
     3241 agtgcatgat gaaagaggtc tgcgcccagt gcctccagag gcacgtcgat cccgccaccg
     3301 ggaaagagaa ggaaatcgtc ttctcctgct tcaaccagga ccagaagttg gacgagatgg
     3361 actgggagaa cctcgccgcg cggttgagac agaacaccgt ttccgagaag ctgacgaacc
     3421 tctggctgga gaggctgttc tcgctgcagg atccgaggct cgtctaagtc ggtctccggg
     3481 aagccgagag aacgaggatc ggcccttcta cggcaccccg gcgaggaaga gaaggtaggg
     3541 gttgatcgcc aggccttccc gcaggttgtc gtcgtcttcg ggcaggcggg tgatcgagaa
     3601 gtgcaggtgc ggccctccga tgacgtgtcc ggtggctccc acgtagccga tgacgtcgcc
     3661 cttggaaacc ttctggccca ccgagaggcc gcccgcgtat cgggacaggt gacagtagaa
     3721 gaagagatac cgtccgctcg aatccttctg gtagatcgtg atcccgccac gccgctcgcg
     3781 ggaaaggcga acgacctcgc cgtcgtcggt cgcgaggatc ggagtgcccc gcggtgcgcc
     3841 gatgtcgatc gccagatgcc gcgcgtactg accccgggac tccaggaaag agtcgcgaag
     3901 gcggtcgcgg tccactcctg acacgggaat gaggaaattc tcggcgagga gctcggacag
     3961 atcgacgtcc tttggcctga cgcgcgccgt cgccgacttc ggccggaaga gcgacccgag
     4021 ccgccgcgcg atcttcgaaa tccgggaaga atcgtgccgg ggagccagga gttccccgtc
     4081 ctccctctcc agaatttcgg tgtcggcgac gtggatgtgc gcttcgatga ccggcggatc
     4141 ttccggaagg cagggagagg gcgggtagtt gtgcgcttcc agcggcgtgg tgggggtgcc
     4201 cgagagaacg tggccgtcgg cggtcaggac ctcgactgag tccagcacga cctcgcccgc
     4261 gtcgacctgg tggatccggg gcgtctcgtg accggcggca gccgtttgcg accgccgcga
     4321 gacccgccct ccagagagcc gctgtgccgt gctgcgcctc gcccgcgcgc gaggagcgct
     4381 ccgcccgacc ccgaccgacc gcttcgaaaa gcctctccca tgacgggaac gtgcttcgga
     4441 gtgggcgggc agaagaaaga gaaacaaagc ggctgccaga gccgccgagg tcaccggcgc
     4501 ctttcgcacg atgggattat tcagtccgct cgaaggcggg gtcaagaaaa aaagacccgc
     4561 agaatcaatt acttatgctg aaacagcata gcgtgtccaa tcgccacaaa cgagggaact
     4621 ccggtgtgga cggtggcgtt cgctccccga gtggggtggg gtgtcgcggg ctctacgcgc
     4681 gttcgaggta gctgttg
//
LOCUS       DASWAV010000606         2912 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U2665
            k141_196752, whole genome shotgun sequence.
ACCESSION   DASWAV010000606 DASWAV010000000
VERSION     DASWAV010000606.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960316
            Sequence Read Archive: SRR10260007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2912)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2912)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:47:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,929
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2912
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_196752"
                     /isolate="SMAG_U2665"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.36444 N 132.31889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..870
                     /locus_tag="VF958_12550"
     CDS             <1..870
                     /locus_tag="VF958_12550"
                     /inference="COORDINATES: protein motif:HMM:NF016214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HEX9149984.1"
                     /translation="LSHPLLDFLNTYGVRFLYPFSKRWFYGDTLFIVDPWVWIALALG
                     IASSRVFRRRRPAQAEAAARVALAAVTAYAAAMMISSFAGRVVVRRAAISAGLVPSGR
                     VLVSPVPLVPWRRLVVVEERAGYRHGTLRWTPRPIVQFAGDLRARNDVGPAVAAAARL
                     RSFRQFLVWARFPYFDVRREGESTLVTAGDARYPGRGGSWAAVSAVVPVSSSVRDSRP
                     RARDGGTDAATIFRMSFTRSATLALTGALAIVSASPAASKEVLPWVDDYGKAVAQARA
                     TNVPIFVEAWAPW"
     gene            886..2016
                     /locus_tag="VF958_12555"
     CDS             886..2016
                     /locus_tag="VF958_12555"
                     /inference="COORDINATES: protein motif:HMM:NF024816.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX9149985.1"
                     /translation="MKAFVFTDKALKRHAGQFVWLSLDVEKAQNAPYKKAYGVDALPM
                     YFIVDPKTEKVALRWVGGATVPQLQKVFADGLVAVKGGGRGVEEVLARADRFYADADY
                     GKAAAEYRQALRVAPSNWSRSARATESLLYALYQTKDWVGCAKTAQDAFPRLKQTSSA
                     ANVAASGLSCALEIPASDASRAQLVKSLGDASREILASPRKDLAADDISAVYETLASE
                     REAAKDEAGKKKVLQERATFLEAAAARAKTPDARAVFDSHRLGTYLDLGEPERAIPML
                     QASERDLPQDYNPPARLAVAYRAMKRYDKALAASDRAIKRAYGPRKLGILQTRADIYK
                     EKGDPAAARRTIEEAVKLAESLPDGQRSDRTIASLKKKLEAM"
     gene            complement(2069..2689)
                     /locus_tag="VF958_12560"
     CDS             complement(2069..2689)
                     /locus_tag="VF958_12560"
                     /inference="COORDINATES: protein motif:HMM:NF019247.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1579 domain-containing protein"
                     /protein_id="HEX9149986.1"
                     /translation="MKLRGILAGLAFLIVIAALAIGADEKGKKTKPAAGKMDEKAMME
                     MMAKYSTPGAEHKKLEAFVGTWDTTAKMWMEPGAPPQESTGVSENKMALGGRYLEQNF
                     EGTMMNQPFSGKGYTGYDIYKKHYIGTWMDTMGTSMMNATGTADPSGKTMTFTGSMDD
                     PMQGKKMNFKEVMTLVDNDHHMFEMWMPGPDGKMFKTLEIHYTRKK"
BASE COUNT          434 a          978 c          949 g          551 t
ORIGIN      
        1 ctgtcccacc cgctgctcga cttcctgaac acgtacgggg tgcggttcct ctatcccttt
       61 tcgaaacgtt ggttctacgg ggacactctc ttcatcgtgg acccgtgggt ctggatcgcg
      121 ctcgcgcttg gaatcgcctc ttcgcgcgtt ttccgaaggc gccgtccggc gcaggcggaa
      181 gccgcggcgc gtgtcgcact cgccgcggtc actgcgtacg cggcggccat gatgatctcg
      241 agcttcgccg ggcgtgtcgt cgtccggcgc gcggcgatct cagccgggct cgtgccctcc
      301 ggtcgcgtgc tcgtttcccc ggttccactc gtcccgtggc ggcgcctggt cgtcgtcgag
      361 gagcgggccg gttatcgcca tggaacgctg cgctggactc cgcggccgat cgtgcaattt
      421 gccggcgatc tccgcgcccg caacgacgtc ggcccggcgg tcgccgccgc cgcgcggctt
      481 cggtcgttcc gccagtttct cgtctgggcg cggtttccgt acttcgacgt ccggcgggaa
      541 ggcgagtcca ccctcgtgac tgccggcgac gcgcgctatc cgggccgggg cggctcatgg
      601 gcggcggtca gcgcagtggt gcccgtgagc tcctcggttc gcgactctcg tcctcgcgct
      661 cgggacggag gcacggatgc tgctacgatc tttcgcatga gcttcactcg ctccgcgacg
      721 ctcgcactga cgggcgcgct cgcgatcgtc tcggcgtcgc cggcggcttc caaagaagtc
      781 cttccctggg tcgacgacta cggcaaggcc gtcgcgcagg cgcgcgcgac gaacgttccg
      841 atcttcgtcg aggcctgggc gccctggtga cacacgtgcc gctcgatgaa ggccttcgtc
      901 ttcacggaca aggccctcaa gcggcacgcc gggcagttcg tctggctctc cctcgacgtc
      961 gagaaggcac agaacgcgcc gtacaagaaa gcgtacggcg tcgacgcgct cccgatgtat
     1021 ttcatcgtcg acccgaagac ggagaaggtc gcgctccgat gggtcggcgg cgcgacggtc
     1081 ccgcagctgc agaaggtctt cgccgacggg ctcgtcgcgg tgaagggcgg cgggcgcgga
     1141 gtcgaagagg tgctcgcgcg cgccgaccgg ttctatgcgg acgccgatta cggcaaggcg
     1201 gcggcggaat acaggcaagc gctccgcgtc gccccctcga actggagccg ttccgcgcgc
     1261 gccacggagt cgcttctcta tgccctctac cagacgaagg actgggtcgg gtgcgcgaag
     1321 acggcacagg acgcgtttcc gcggctgaag cagacctcct cggcggccaa cgtcgcggcg
     1381 agcggcctct cgtgcgcgct cgagataccg gcgtcagacg cgtcgcgcgc acagctcgtc
     1441 aagtcgctcg gcgatgcgtc gcgcgagatc ctcgcgtcgc cgcgtaagga cctcgcagcg
     1501 gatgacatct ccgcggttta cgagaccctg gcgtccgagc gggaggccgc gaaggacgaa
     1561 gcgggtaaga agaaggtcct gcaggagcgc gcgacgtttc tcgaagcggc ggcggcgcgt
     1621 gcgaagaccc ccgatgcgcg cgccgtcttc gactctcacc gcctcgggac ctacctcgat
     1681 ctcggcgaac ccgaacgcgc gatcccgatg ctgcaggcgt ccgagcgcga ccttccgcag
     1741 gactacaacc cgccggcccg gctcgccgtc gcttaccggg cgatgaagag gtacgacaag
     1801 gcgctcgcgg cgtccgatcg cgcgatcaag agggcgtatg ggccccgcaa gctcgggatc
     1861 ctccagacgc gcgccgacat ctacaaggag aagggcgatc ctgccgcggc gcgccggacg
     1921 atcgaggaag ccgtgaagct ggccgaatcg ctgcccgacg gacagcggtc ggaccggacg
     1981 atcgcgtcgc tcaagaagaa gctggaagca atgtgaaaaa ggcgggccga ggcccgcctt
     2041 ttgtccgagc gtgcgctctc gcgcgaggtc acttctttcg cgtgtagtgg atttccagtg
     2101 tcttgaacat cttgccgtcc gggcccggca tccacatctc gaacatgtga tggtcgttgt
     2161 ccacgagggt catgacttcc ttgaagttca ttttcttgcc ctgcatggga tcgtccatcg
     2221 aaccagtgaa cgtcatcgtc ttccccgacg ggtccgcggt gccggtcgcg ttcatcatgc
     2281 tcgtacccat cgtgtccatc cacgtgccga tgtagtgctt cttgtaaatg tcgtagccgg
     2341 tgtagccctt cccggagaag ggctggttca tcatcgtccc ctcgaagttc tgctcgaggt
     2401 accggccacc gagcgccatc ttgttctccg aaacgcccgt gctctcctgc ggcggcgcgc
     2461 ccggctccat ccacatcttg gcggtcgtat cccacgtccc gacgaatgcc tcgagcttct
     2521 tgtgttcggc gccgggcgtc gagtacttcg ccatcatttc catcatggct ttctcgtcca
     2581 tcttgccggc ggccggcttg gtctttttgc ccttctcgtc cgctccgatg gcgagagcgg
     2641 cgatgacgat cagaaaggcg aggccggcca ggattccccg aagtttcatg ctggttcctc
     2701 ctttggtccc atgcgggact tttcgaagag tcgggagttc tacgggggga ttctatgcgg
     2761 ggcggccggc caaaaaaaaa gcacgggact ccttcgagcc cctggctggc taactgtgtg
     2821 gattcgagga gttagcggcc gaaacgctcc cgcatcccgc cccgcccctg gaagcgctcc
     2881 tggcgcttct gcctcatcgt atcgagcttg tc
//
LOCUS       DASWAV010000607         7301 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U2665
            k141_316171, whole genome shotgun sequence.
ACCESSION   DASWAV010000607 DASWAV010000000
VERSION     DASWAV010000607.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960316
            Sequence Read Archive: SRR10260007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 7301)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7301)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:47:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,929
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7301
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_316171"
                     /isolate="SMAG_U2665"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.36444 N 132.31889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..331)
                     /locus_tag="VF958_12565"
     CDS             complement(<1..331)
                     /locus_tag="VF958_12565"
                     /inference="COORDINATES: protein motif:HMM:NF036808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9149987.1"
                     /translation="MARFDARKAAISRIRLGVESAIDARAAASVESPESISSFRWPRS
                     RGLFFFESGVYFGFVIRLVLKPGRDESLRRRHPWVFSGAIACATGDGSDGVAEVVDSS
                     GQLVGCGA"
     gene            complement(265..993)
                     /locus_tag="VF958_12570"
     CDS             complement(265..993)
                     /locus_tag="VF958_12570"
                     /inference="COORDINATES: protein motif:HMM:NF016658.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF547 domain-containing protein"
                     /protein_id="HEX9149988.1"
                     /translation="MNALAAAALLLAASLSPQPEGVPGPAPLDAVLAARARGGGFDYR
                     GAGGQDRTRLAAYLSNLGDARPEQMSSDEKKALYINAYNAMAIAIVLDRDPVKSIMEI
                     DGAFRKIRRRIGGRELSLDEIEDRLRELSDPRIHFAIVCASESCPPLAQRAYEPRTIS
                     AALDRQARAFVNDPSKNVVDRPRNRIALSKILDWNRTEFERDAGSLTKFVARYVADGE
                     TASWLASTRERPQFLEYDWALNQR"
     gene            complement(1007..2041)
                     /locus_tag="VF958_12575"
     CDS             complement(1007..2041)
                     /locus_tag="VF958_12575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015894184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anion permease"
                     /protein_id="HEX9149989.1"
                     /translation="MTYVLLIIVVALVFDFINGMHDSANSIATVVSTRVLSPLLAVFW
                     AAFFNFAAAFLFGTAVAKTIGTGLIDVTVVNSNVVLGGLLGAISWNLITWYFGLPSSS
                     SHALIGGYAGAAVARVGVRAIVPGAAWTKTLAFIVLSPILGMTAGFALMVGMSWLFHR
                     APYGSVDRWFRRAQLGSAAAFSLSHGTNDAQKTMGIIGGLLVANQAIFADPSSALHWM
                     YLPAIKKQLPWWIILSAHAAIGLGTLTGGWRIVRTMGSRITKLKPFSGFCAETAAATT
                     VIGASLAGIPVSTTHTITGAIMGVGSTQRFSAVRWGVAGQIVYAWILTIPMAAVIGAL
                     AYGILSVFAP"
     gene            complement(2086..2700)
                     /locus_tag="VF958_12580"
     CDS             complement(2086..2700)
                     /locus_tag="VF958_12580"
                     /inference="COORDINATES: protein motif:HMM:NF013982.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF47 family protein"
                     /protein_id="HEX9149990.1"
                     /translation="MLNVLPRDEKYFDRFNELAQRTHESAVMLHRFFTGEAAVPAVAD
                     QIKRLEHECDQISHEILHGIDRTFITPIDREDIHQLAVRLDDVIDLIDGTIRRLAIFN
                     ISEPTVAGRKLSEIIVKITLEMVEAVAALRKQKGVVEHCIRMKQFENEGDVAYQEAVG
                     SLFRQAIAPIEVIKWKDVYENMEGCIDQAEAVAHVLESVVLKHS"
     gene            complement(2818..4290)
                     /locus_tag="VF958_12585"
     CDS             complement(2818..4290)
                     /locus_tag="VF958_12585"
                     /inference="COORDINATES: protein motif:HMM:NF019095.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHRD domain-containing protein"
                     /protein_id="HEX9149991.1"
                     /translation="MRKTLFLVLVFVSCLSVAAGAQTFVGVMNGTNEAPGPGDADGFG
                     LAGFRFEGTSVAFTIMVKNIATPTASHIHRGAAGVPGPVVITLASSFPNNTASGTANA
                     SAALIDEIRHNLSGFYVNVHNADFPNGAIRAQLSDGAFAFATGANEAPGPGDADGSGM
                     AVFIGSGSNLNYAAMVQNIDAPSASHIHRGAVGVPGPVVITLASSYPDNMATGTVTIA
                     DTLIQEIVGNPSNFYFNVHNAAFPNGAIRGQLTLAPYTEAHYFPIVGRVDGLNNTRFV
                     ADARVVNRSSETENVTLEFFQSSSAGLSAASAVRGVQVAPGAELVINDVVGSQFSATG
                     LGALKIGTSSEQTAGVRVFNDLRPADGGTTGFFIRSKEISGAATSGVLPFLSQASDAQ
                     IQTGDGFRSNIGWFNPNLADAGATFQVHRASDGAVLGSVDVGIAGLSQLQQAVFSLVS
                     TVAAGDRVQSDFYVTWTSTRPIYVYGAVVDNKTGDVVYVD"
     gene            complement(4570..>7301)
                     /locus_tag="VF958_12590"
     CDS             complement(4570..>7301)
                     /locus_tag="VF958_12590"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEX9149992.1"
                     /translation="GFSAGRVGNVVVTVGGDANVKLVLEPAGVQAAVTVSSEAPLIET
                     TKSSVSSVVDERMISNLPTNGRNFIDFVLSTPGVVRDEARLGDIVFAGQRGTLNSLVV
                     DGADNNNTFFGQALGRTGSGRAPYQFSQDAVKEFQVNTSAYSAEYGRAGGAVINVVTK
                     SGTNEFHGGAFYFYRDKSIKSKDYIDVVNHRPETPYHFDQFGASLGGPILRDRLFFFA
                     NYDGQRNTTPNTVVLGLPANTPTDPDTAAGVARLSGLAFDWDRKQDQDVYLLKTDYEV
                     SSRHHLSLRYNRQDFTGQGFENGGTQNSFEHTGDSLVKTDTFGGSFASSLTQNLFNEL
                     RGQYAKDSEPGTANSANPEATVRQGGALVLTIGRNFFSPRETTIKRYQFADTATWLFG
                     NHTLKGGFDYNKDDILNFFPGNFSGAYTFASIASFQRGAPNGTGERYVQAFPGPGTSG
                     ATTNPNLIDYAGFVEDEWRVTPAVTLNLGLRYDFQDIASGSVRNPDPQLAAAGLDTRN
                     VPDDTNNWAPRLGIAWDVGRDGRTVVRGGYGIFYGRTTGIMVGTAHSNNGINVQTITF
                     TGSQVPTYPNTFASIPSGAAIPKPTIFVFEPDFQSPKVQQASLGIDRAITNDIGIALS
                     YLYVKGEDLPRSTDINLGPASIVDVPIVGNGTVPVKRYGTPRPFANFDRVIEFQSSAE
                     SEYNGLTLELVKRFSHGWQARLAYTLGRVTDTKPDATAVVPQGSDDNKYISDPLDFGV
                     DDAIGDLDARHRVVLSGVWDLGDAAGISNGVVRFLASNWMLSGVFIVQSGQPYSALVN
                     GDLNGDGNTRNDRAPEFERNSFRLPTQVSFNPRVAKRIPIGPVSLELIGEAFNLFNRS
                     NAIGVQQNYYTFANGSLTRVTTFGTPCAGGLPCTAVNASTGPRTYQLAAKVTF"
BASE COUNT         1261 a         2352 c         2430 g         1258 t
ORIGIN      
        1 acgcgccgca cccgacgagc tggccgctcg agtcgacgac ctcggcgacg ccatccgagc
       61 cgtcacccgt cgcgcacgcg attgcgcccg agaagaccca gggatggcgg cggcgcaggg
      121 actcgtctcg gccgggtttc aagacgagtc ggatcacgaa gccaaaatac acgcccgact
      181 cgaaaaagaa gaggccccgc gagcggggcc atcggaaaga ggagatcgat tctgggcttt
      241 ctacggaggc cgcggctcgc gcgtctatcg ctgattcaac gcccagtcgt attcgagaaa
      301 ttgcggcctt tcgcgcgtcg aagcgagcca cgaggccgtc tccccgtcgg cgacgtatcg
      361 cgcgacgaac ttcgtcaagc tccccgcgtc ccgctcgaat tcggtccggt tccagtcgag
      421 aatcttcgac agggcgatcc ggttccgggg ccggtcgacg acgtttttcg acgggtcgtt
      481 gacgaacgcg cgggcctgac ggtcgagggc ggcggagatc gtccgcggct cgtaggccct
      541 ctgcgccagc ggcgggcagg actccgacgc gcacacgatg gcgaagtgaa tgcgcggatc
      601 gctcagttcc cgcagccggt cctcgatttc gtcgagcgag agctcgcgtc cgccgatcct
      661 ccgtcggatc ttccggaacg cgccgtcgat ctccatgatg ctcttcaccg ggtcgcggtc
      721 gagcacgatc gcgatggcca tggcgttgta agcgttgatg tagagcgcct tcttctcgtc
      781 ggacgacatc tgttccgggc gcgcgtcccc gaggttcgag aggtacgcgg cgaggcgcgt
      841 gcggtcctgc ccgcccgctc cccggtagtc gaatccgccg ccgcgcgctc gggccgcgag
      901 caccgcgtcg agcggcgcgg gaccggggac gccttccggc tgcggcgaga gagacgccgc
      961 caggaggagc gccgccgcgg cgagagcgtt caccgccacc cgccgctcaa ggggcgaaga
     1021 ccgagaggat cccgtacgcc agggcgccaa tcaccgccgc catcggaatc gtgaggatcc
     1081 aggcgtagac gatctggccg gcgacccccc agcgcaccgc cgagaagcgc tgcgtcgagc
     1141 cgacccccat gatcgcgccg gtgatcgtgt gcgtcgtcga caccgggatt cccgcgagcg
     1201 acgccccgat cacggtcgtc gccgcggccg tctccgcgca gaaaccggag aacggcttca
     1261 gtttcgtgat gcggctgccc atcgtccgga cgatgcgcca accgccggtc agcgtgccga
     1321 ggccgatcgc cgcgtgcgcg gagaggatga tccaccatgg cagctgtttc ttgatggcgg
     1381 gcaggtacat ccagtgcaaa gcactcgagg gatccgcaaa aatcgcctgg ttcgcgacga
     1441 gaagtccccc gatgatcccc atggtcttct gcgcgtcgtt cgtcccgtgg gagagggaga
     1501 acgcggccgc ggacccgagt tgcgcgcgac ggaaccagcg gtcgaccgac ccgtacgggg
     1561 cccggtgaaa gagccacgac attccgacca tgagcgcaaa gccggccgtc attccgagga
     1621 tcggggacag gacgatgaac gcgagcgtct tcgtccaggc ggcgcccggg acgatcgcgc
     1681 ggacgccgac tctcgccacg gcggctcccg catagccccc gatgagcgca tgggaagagg
     1741 atgatgggag tccgaaatac cacgtgatca ggttccagct gatcgcgccg agcagaccgc
     1801 ccaggacgac gttcgagttg accacggtga cgtcgatcaa ccccgttccg atggtcttcg
     1861 caactgccgt gccgaacagg aacgccgcgg cgaagttgaa aaaggcggcc cagaagacgg
     1921 caaggagagg cgaaagcacg cgcgtcgata cgaccgtggc gatcgagttc gccgagtcgt
     1981 gcatcccgtt gatgaagtcg aagacgagcg cgacgacaat gatcagaaga acgtaggtca
     2041 tcgcgcagct ctcgcttccg gccgtcagct attcgccgcg tgccctcacg aatgcttcag
     2101 gacgacgctc tcgagaacgt gggcgactgc ctcggcctgg tcgatgcagc cctccatgtt
     2161 ctcgtagacg tctttccact tgatgacctc gatcggcgcg atcgcctgcc gaaacagcga
     2221 gccgaccgcc tcctggtagg cgacgtcccc ctcgttctcg aactgcttca tgcggatgca
     2281 gtgctcgacc acgcccttct gttttctcag ggcggccacc gcctcgacca tctcgagcgt
     2341 gatcttcacg atgatctcgg aaagcttgcg gccggcgacc gtgggctccg agatgttgaa
     2401 gatcgcgagg cgacggatcg ttccgtcgat caagtcgatc acgtcgtcga ggcggacggc
     2461 cagctggtgg atgtcctcac ggtcgatcgg cgtgatgaac gtccgatcga ttccgtgcag
     2521 gatctcgtgc gagatctggt cgcactcgtg ttcgaggcgc ttgatctggt cggcgacggc
     2581 cggcacggcc gcctcgccgg tgaagaaccg gtgcagcatc acggcggatt cgtgcgttcg
     2641 ctgcgccagc tcgttgaagc ggtcgaagta tttctcgtcg cgcggaagga cgttcaacat
     2701 cggccgcctc cgtggttcgc gcgcgatgct accccaagtt ccgcggccgc ccgccgggca
     2761 agagaaaacc cggcgccgcg gcgccgggtt cggaaaccga gaaccgaaaa ttgagaatta
     2821 gtcgacgtac accacgtctc cggtcttgtt gtcgaccacc gcgccgtaga cgtagatcgg
     2881 gcgcgtcgaa gtccaggtga cgtagaagtc tgactgcact cgatctcccg ccgcgacagt
     2941 cgagaccagc gagaacaccg cctgctggag ctgggagagc ccggcgatcc cgacgtcaac
     3001 agatccgaga acagcgccgt cgctcgcgcg atggacctga aacgtcgctc ccgcgtccgc
     3061 gagattcggg ttgaaccagc cgatgttcga acggaagccg tcccccgtct ggatctgggc
     3121 atcgctcgcc tgtgagagga aaggcaggac gccgctcgtc gcggctccgg agatctcttt
     3181 ggaacggatg aagaatcccg tcgttccccc gtcggccgga cggagatcgt tgaagacccg
     3241 aactccggcc gtctgttcgc tcgacgtacc gatcttcaat gctccgagac cggtcgccga
     3301 gaactgcgac ccgacgacgt cgttgatcac gagctccgcc cccggagcga cctggacccc
     3361 gcgcaccgcg gatgcggcgg acagccctgc agaagagctc tggaagaact cgagcgtcac
     3421 gttctccgtc tcgctcgaac ggttcacgac gcgcgcgtct gccacgaaac gcgtgttgtt
     3481 caagccgtcc actcttccaa cgatcgggaa gtaatgagct tcggtgtacg gcgcgagggt
     3541 gagctgcccg cgaatcgcgc cgttcgggaa agcggcattg tggacattga agtagaagtt
     3601 gctcgggttc ccgacgatct cctgaatgag tgtgtcggcg atggtcaccg ttccggtcgc
     3661 catgttgtcc gggtaggagc tcgcgagcgt gatcacgact ggtcccggaa ctcccactgc
     3721 cccccggtgg atgtgcgacg cgctcggcgc gtcgatgttc tggaccatcg cggcgtagtt
     3781 caaattcgag cccgacccga tgaagaccgc catgcccgac ccgtctgcgt cgcccggtcc
     3841 gggagcctca ttggcgcccg tcgcgaacgc gaacgcgccg tcggagagtt gcgctcggat
     3901 cgcgccgttg gggaagtcgg cgttgtgaac gttgacgtag aacccggaaa gattgtgccg
     3961 gatctcgtcg atcagcgcgg cgctcgcgtt cgccgttccc gaagcagtgt tgttcgggaa
     4021 ggaggaggcg agcgtgatga cgacggggcc cgggactccg gcggcacctc gatgaatgtg
     4081 cgaagccgtc ggcgtcgcga tgttcttcac catgatggtg aacgcgacgg acgttccttc
     4141 gaagcggaac cccgcgagac cgaagccgtc ggcgtctccg gggcccggag cctcgttagt
     4201 gccgttcatg acgccgacga aggtttgcgc ccccgcggcc accgagagac acgagacgaa
     4261 aacgaggacc agaaacagag tcttgcgcat ggatctcctt cctttcgtgc ggccgccgga
     4321 cccggagcgg ccggggtgat gcgaaacggg gccggaagcg gctccgtgcg gattcgacat
     4381 cgtcttgggc gtcgctcttc gaaattgagg agacgttaag cgtgtccgat ggaacgagac
     4441 gacactagtc ggaaactctg tccggggcct gatgcacctt acccgaggcg ccggcgtgat
     4501 cgcctgccgc ccacgaccga agaaaaggcg cggccgcagc ggcgcccgtc gaccaacccg
     4561 gccgggcgac tagaaagtga ccttcgccgc cagctggtac gttcgcggcc cggtcgacgc
     4621 gttcacggcg gtgcagggca ggccccccgc gcacggcgtt ccgaacgtcg tgactctcgt
     4681 cagagaaccg ttcgcgaacg tgtagtagtt ctgctgcact ccgatcgcgt tcgatcggtt
     4741 gaagaggttg aacgcctctc cgatgagctc gaggctcacg gggccgatcg ggattcgctt
     4801 ggccacccgc ggattgaacg agacctgcgt cggcaggcgg aagctgttcc gctcgaattc
     4861 gggagcccgg tcgttccggg tgttgccgtc gccgttcagg tctccgttga cgagagccga
     4921 gtagggctgg cccgactgga cgatgaagac gcccgagagc atccagttcg acgcgagaaa
     4981 acgcactacg ccattgctga tcccggcggc gtcgccgagg tcccagacgc ccgagagaac
     5041 cacgcggtgc cgggcgtcca ggtcgccgat cgcgtcgtcc acaccgaagt ccagcggatc
     5101 cgagatgtac ttgttgtcgt cggacccctg gggaacgacc gccgtggcat ccggcttcgt
     5161 gtccgtgacc ctgccgagcg tgtaggcgag ccgtgcctgc cacccgtggg agaaccgctt
     5221 cacgagctcg agggtgagac cgttgtattc cgactctgcg gagctctgga actcgatgac
     5281 ccggtcgaaa ttcgcaaaag gccggggagt gccgtaccgc ttgaccggca cggtgccgtt
     5341 cccgacgatc gggacgtcga cgatcgacgc cgggccgagg ttgatgtccg tcgaacgggg
     5401 aaggtcttcg cccttcacgt acaggtacga aagcgcgatg ccgatgtcgt tcgtgatcgc
     5461 tcgatcgatt cccaggctcg cctgctgaac tttcgggctc tggaagtccg gctcgaacac
     5521 gaaaatcgtc ggcttgggga tcgccgcccc cgaggggatc gacgcgaaag tgttcgggta
     5581 cgtcggcacc tggctgccgg tgaacgtgat cgtctgcacg ttgatcccgt tgttcgagtg
     5641 cgccgtgccg accatgatcc cggtcgtccg gccgtagaag atcccgtacc ccccccgcac
     5701 gaccgtgcgg ccgtcgcggc cgacgtccca cgcgatcccg agccggggcg cccagttgtt
     5761 cgtgtcatcg ggaacgttcc gggtatcgag accggccgcg gcgagctgcg ggtcggggtt
     5821 ccggacgctt ccgctggcga tgtcctggaa gtcgtaccgc aatcccaggt tgagcgtcac
     5881 ggccggcgtg acgcgccact cgtcctccac gaaacccgcg tagtcgatca ggttcgggtt
     5941 cgtcgtggcc cccgacgtgc ccggaccggg aaacgcctgg acgtaccgct cccccgttcc
     6001 gttcggggcg ccgcgctgga acgacgcgat gctcgcgaag gtgtaggcgc ccgagaagtt
     6061 accgggaaag aagttcagga tgtcgtcctt gttgtagtcg aagcccccct tcaacgtgtg
     6121 gtttccgaag agccacgtcg ccgtgtcggc gaactggtac cgcttgatcg tcgtctcgcg
     6181 cggcgagaag aagttgcggc cgatggtcag gacgagagcc ccgccctgac ggacggtggc
     6241 ctcgggattc gcgctgttgg cggtcccggg ctcgctgtcc ttcgcatact gtccgcgcag
     6301 ctcgttgaac aggttctgag tgagcgagct cgcgaatgat ccgccgaacg tgtcggtctt
     6361 cacgagcgaa tcgcccgtgt gctcgaagga gttctgcgtc cctccgttct cgaagccctg
     6421 gcccgtgaag tcctgacggt tgtagcgcag cgaaaggtga tgccgcgaag acacctcgta
     6481 gtcggtcttc aagaggtaga catcctggtc ctgcttgcgg tcccagtcga aagcaaggcc
     6541 cgagagacgc gcgacgccgg cggccgtgtc cgggtccgtc ggcgtgttcg cgggcaggcc
     6601 cagaacgacg gtgttcgggg tcgtgttgcg ctgaccgtcg tagttcgcga agaagaacag
     6661 gcggtcgcgc aggatcggtc cgccgaggct cgccccgaac tggtcgaagt ggtacggcgt
     6721 ctccgggcgg tggttgacga cgtcgatgta gtccttcgac ttgatgctct tgtcgcggta
     6781 aaagtagaac gctccgccgt ggaattcgtt cgtgcccgac ttcgtcacga cgttgatcac
     6841 cgcgcctccg gcgcgtccgt actcggcgga gtaggcgctg gtgttgacct ggaactcttt
     6901 gaccgcgtcc tggctgaact ggtacggagc gcggcccgag ccggtgcgtc cgagcgcctg
     6961 cccgaagaag gtgttgttgt tgtccgcgcc gtcgacgacg aggctgttca aagtcccccg
     7021 ctgaccggcg aagacgatgt cgcccagccg cgcctcgtcg cggacgactc cgggcgtcga
     7081 cagcacgaag tcgatgaagt tgcggccgtt cgtcggaagg ttagagatca tccgctcgtc
     7141 cacgaccgag ctcacggagg acttcgtcgt ttcgatgagc ggggcctcgc tcgacaccgt
     7201 caccgcggcc tggacacccg cgggctcgag cacgagcttg acgttcgcgt ccccgccgac
     7261 ggtgaccacg acgttcccga cgcgcccggc gctgaagccc g
//
LOCUS       DASWAV010000608         4250 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U2665
            k141_516619, whole genome shotgun sequence.
ACCESSION   DASWAV010000608 DASWAV010000000
VERSION     DASWAV010000608.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960316
            Sequence Read Archive: SRR10260007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 4250)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4250)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:47:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,929
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4250
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_516619"
                     /isolate="SMAG_U2665"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.36444 N 132.31889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..213)
                     /locus_tag="VF958_12595"
     CDS             complement(<1..213)
                     /locus_tag="VF958_12595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020712768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9149993.1"
                     /translation="MNFSAMMKQPSAFLPVAMSLAALAVVLGHILVFGVAREADEGTA
                     AHLWQLLIGGQLPVVAFFAIKWLPRAP"
     gene            249..2501
                     /gene="rnr"
                     /locus_tag="VF958_12600"
     CDS             249..2501
                     /gene="rnr"
                     /locus_tag="VF958_12600"
                     /inference="COORDINATES: protein motif:HMM:TIGR02063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008997 - ribonuclease R activity
                     [Evidence IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HEX9149994.1"
                     /translation="MAVVRPVAELPSGASDTLLRVLPRDSEIVDKLREKGARILSFRE
                     LIQGLKVREEEEEPLRERLEALERRGEIVRVRGEKYSAIEFTNQVAGRITVRPEGFGF
                     VLVDDGPDLFIPRGGMHGAMDGDTVLAREERARSRSRAGGPERISGTVVRVLDRARER
                     VVGRFEKQEGRAVVLPYDPRIDAIVRIEDGRARGARVGEIVDVRLTSFPDARRFAHGV
                     VEERLGFLGEPGVDIEIVLRSHGLPPRFPKPVIEEAERFLPRVRTEDLLGRRDFRSRR
                     IVTIDGETAKDFDDAIEVERTATGFRLGVHIADVSHYVKEGTALDDEARSRGTSVYFP
                     GRVLPMLPEHLSNGLCSLNPGVDRLVLSALLETDRKGRILSAEVVKGVIKSSERMTYT
                     EVARLLENRPTHGDKERYGVLLQDFREMGELAARLRERREARGSIDFDLPDADIVLDD
                     EGLVVGIVPESRNVAHRLIEEFMLAANEAVAKKLLFAKQPAIYRVHDRPDPDRLVDLR
                     EVLDGFGYELKGDLDEVPPAAFQKILNAIAGKPEERLLSDLLLRAQRKALYSEECRGH
                     YALAAPYYCHFTSPIRRYPDLIVHRQLSTLLETGRPVAPKDFDAVNENLRELAALSSE
                     RERRAEAAERESLLWKKIVFMKDKIGRDFDAYVTGVTSFGLFVMLRDFFVEGLVPVSS
                     LPDDFYVYEEKQHRLRGRSSGKIFRLGDAMRVQLKAIDEVRRRLDFRVPGVERPRAEH
                     AVRRTRSARQ"
     gene            2498..3013
                     /locus_tag="VF958_12605"
     CDS             2498..3013
                     /locus_tag="VF958_12605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9149995.1"
                     /translation="MRRAAAALIAFAILTVACRERGRPDPNVRMAVSHEHEAETGANS
                     EKGAPVPTKLVVPPEVTAAFSGVQIGWKDSQSGQGGILDVPLGGSAPIPGSDLAVKAD
                     VFLPSFTMTAEQITSTGIALENPAARIVVVQGEKEVFTGWIFKRFPDVHPFQHPRFSL
                     RLEGGVPRNKA"
     gene            3010..>4250
                     /gene="der"
                     /locus_tag="VF958_12610"
     CDS             3010..>4250
                     /gene="der"
                     /locus_tag="VF958_12610"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HEX9149996.1"
                     /translation="MSAFRVVVAGRPNVGKSALVNRLLRRRRSIVHDKAGMTRDVLDV
                     EAKLPDGRSYRLLDTGGYDPEGREKIPAAVREKALGSIRSADLVLLVMDASAGVLPGD
                     RSAARVAREAGVETVVVANKIDRREGSEGEVEAWELGFPEVYGVSAEHGIGIDELVEA
                     IAGRIPAPSRESENEKRETAGENEEKEERPFEIRLAVVGRPNVGKSSLVNALLGEDRT
                     IVSEIPGTTRDAVDVLLERGGKTFRLVDTAGIRRKAKTERGPEVLSVVQARKRIEECD
                     VALMVLDAVEGPTGQDAAVAAYAHESGKGLVIVANKWDLAGRASAEASKVFEADVRER
                     MPFAGYAEILRASALTGRGVRRILAVASRAAENRRRRITTGELNRVLGRVLRDSPPRT
                     ARGTRLRVYYVAQTDVAPPTFT"
BASE COUNT          738 a         1375 c         1474 g          663 t
ORIGIN      
        1 gggagcccgc ggcagccact tgatcgcgaa gaatgcgacg acgggcagct gtccgccgat
       61 gagcagctgc cagagatggg ctgcggttcc ctcatctgcc tcacgcgcaa cgccgaagac
      121 gagaatgtga ccaagcacca cggcaagggc agcgagcgac atggccaccg gaagaaaggc
      181 gctcggctgc ttcatcattg ctgagaagtt cattcggcag cctccagaat atttcaaaca
      241 cctcacgcat ggcggtggtc cggccggtcg ccgaactccc ctcgggagcg tcggatacac
      301 tcctgcgcgt gttgccgcgc gattccgaga tcgtcgacaa gctgcgggaa aagggggcgc
      361 gaatcctctc ttttcgcgag ctcatccagg ggctgaaagt ccgcgaagag gaagaggagc
      421 cccttcgcga gcgactcgag gccctcgagc ggcgcggcga gatcgttcgc gtccgcggcg
      481 agaaatactc ggcgatcgag tttacgaacc aggtcgcggg aagaatcacc gtccgacccg
      541 agggcttcgg gttcgtcctc gttgacgacg gtccggacct cttcataccg cggggcggga
      601 tgcacggcgc catggacggc gacacggttc tcgcgcggga ggagcgcgca cgctcacgaa
      661 gccgcgccgg cggcccggag cggatctcag gtaccgtggt ccgggtcctc gaccgtgcgc
      721 gcgagcgcgt cgtcggacgc ttcgaaaagc aggagggacg tgcggtggtg ctcccgtacg
      781 acccgagaat cgacgctatc gtgcgaatcg aggatggacg ggcccggggc gcgcgggtgg
      841 gggagatcgt cgacgttcga ttgacgtcgt tccccgatgc tcggcgcttc gctcacggcg
      901 tggtggaaga gcggctcggg ttcctcggcg agcccggagt cgacatcgag atcgtcctgc
      961 gctctcacgg ccttccgcca cggttcccga agcccgtgat cgaagaggcg gaacgctttc
     1021 tgccgcgcgt gcgcaccgag gaccttctgg gccggcgaga tttccggagc cgacggatcg
     1081 tgacgatcga cggcgagacc gcgaaggact tcgacgacgc gatcgaggtc gaacgaaccg
     1141 cgacgggatt ccgtctcggg gtccacatcg ccgacgtctc gcactacgtc aaggagggca
     1201 ccgcgctcga cgacgaggcg cgatcgcggg ggacgagcgt ttacttcccc ggccgcgtcc
     1261 tccccatgct tcccgagcat ctctccaacg gactctgctc gctcaacccg ggagtcgacc
     1321 ggctcgtcct gtcggctctg ctcgagaccg accgaaaggg ccggatcctc tcggccgaag
     1381 tcgtcaaagg cgtcatcaag agctccgaac gcatgacgta cacggaggtc gcgagactgc
     1441 tcgagaaccg ccccacccac ggcgacaagg agcggtacgg cgtccttctg caggactttc
     1501 gcgaaatggg ggagctcgcc gcgcggcttc gcgagcggcg cgaggctcgt gggtcgatcg
     1561 atttcgatct gcccgacgcc gacatcgtgc tcgacgacga gggtctcgtg gtcggaatcg
     1621 tgcccgagtc gcgcaacgtc gcccaccggc tgatcgagga gttcatgctc gctgcgaacg
     1681 aggccgtggc gaagaagctt ctcttcgcga agcagccggc gatctatcgc gttcacgacc
     1741 gcccggatcc ggaccgtctc gttgacctgc gcgaagtgct cgacggcttc ggatacgaac
     1801 tgaagggcga tctcgacgag gtgccgccgg cggccttcca gaagatcctg aacgcgattg
     1861 ccggcaaacc ggaagagcga ctcctctccg acctcctcct tcgcgcacag cgcaaagccc
     1921 tctacagcga ggagtgccgg ggacactacg cgctcgccgc gccttactac tgtcatttca
     1981 cttcgccgat ccgccggtat ccggacctga tcgttcatcg gcagctctcg acgcttctcg
     2041 agacgggacg ccccgtcgcg ccgaaggact tcgacgccgt caacgagaat ctgcgagagc
     2101 tcgccgcgct gtcgtcggag agagaacgcc gcgccgaagc ggcggaaagg gagagcctgc
     2161 tgtggaaaaa gatcgttttc atgaaggaca agatcggccg cgatttcgac gcttacgtca
     2221 ccggggtgac ctcgttcggg ctattcgtca tgctgcggga cttcttcgtc gaggggctgg
     2281 tgccggtgtc gagcctcccc gacgacttct acgtctacga ggagaagcag caccgcctcc
     2341 gcgggcgctc gagcggaaag atctttcgcc tcggcgacgc gatgcgagtt cagctcaaag
     2401 cgatcgacga agtgcggcgg cgtctcgact tccgcgttcc gggcgtggag cgcccgcggg
     2461 cggagcacgc ggtgcgacga acccggagcg cgcggcaatg aggcgcgcgg ccgccgccct
     2521 catcgcgttc gcaatcctca ccgtggcgtg ccgcgagcga ggtcgtcccg acccgaacgt
     2581 ccggatggcg gtctctcacg agcacgaggc ggaaaccggt gcgaactcgg agaagggcgc
     2641 tccggttccg acgaagctcg tcgtcccgcc cgaagtcacc gcagctttct ccggggtgca
     2701 gatcgggtgg aaggactccc agagcggcca ggggggaatt ctcgacgtgc cgctcggcgg
     2761 ctccgcgccg atccccggct cggatctcgc ggtgaaggcg gacgtctttc tcccgtcgtt
     2821 cacgatgact gccgagcaga tcacttccac gggaatcgca ctcgagaacc cggccgcgcg
     2881 gatcgtcgtc gttcaggggg aaaaggaggt cttcaccggc tggatcttca agcggttccc
     2941 ggacgtgcat ccgttccagc acccccggtt ctctctacgg ctcgagggag gcgtcccgcg
     3001 aaacaaggcg tgagcgcctt tcgggtcgtg gtcgccggcc ggccgaacgt cggtaagtcc
     3061 gccctcgtaa accggctgct ccgtcgacgg cggtcgatcg tccacgacaa ggccggcatg
     3121 acgcgcgacg ttctcgacgt cgaagcgaag cttcccgacg gccgcagcta ccgcctgctc
     3181 gataccggcg gttacgaccc ggaggggcgg gagaagatcc ccgccgcggt gcgcgagaag
     3241 gcgctggggt cgatccgcag cgccgacctc gttctgctcg tcatggacgc ttccgcgggc
     3301 gtcttgccgg gcgatcgaag cgcggcgcgg gtcgcgcggg aggccggagt cgagaccgtc
     3361 gtcgtcgcga acaagatcga ccggcgtgag ggaagtgaag gggaggtcga ggcgtgggag
     3421 ctcggattcc ccgaggtgta cggcgtgtcg gccgagcacg ggatcggaat cgacgagctg
     3481 gtcgaggcaa tcgcggggcg aatccccgct ccgagccggg aatcggaaaa cgaaaaacgg
     3541 gaaacggcgg gggagaacga ggagaaagag gaacgtcctt tcgagatccg cctggccgtc
     3601 gtcgggcggc cgaacgtcgg gaagtcttcg ctcgtcaacg cgctgctggg cgaggaccga
     3661 acgatcgtct cggagattcc gggcacgacg cgcgacgcgg tcgacgtcct cctcgagcgg
     3721 ggcggaaaga cttttcgtct cgtggacacc gccggcatcc ggcgaaaggc gaagacggaa
     3781 cgggggccgg aagtgctgtc cgtcgtgcag gcccgcaagc ggatcgagga gtgcgacgtc
     3841 gccctgatgg tcctcgacgc cgtggaggga cccactggtc aggatgcggc ggtcgccgcc
     3901 tacgcccacg agtcgggcaa gggtctcgtc atcgtcgcga acaagtggga cctggcaggg
     3961 agggcttcgg ccgaagcctc gaaagtgttt gaagccgacg tgcgcgagcg gatgcccttt
     4021 gccggatacg ccgagatcct gcgagcctcg gcgctgacgg ggcggggggt ccgccggatc
     4081 ctcgccgtcg cctcccgggc ggccgagaac cgccgccgcc gcatcacgac gggggagctg
     4141 aatcgcgttc tcgggcgggt tctccgggac tcgccgcccc gcaccgcccg gggcacccgc
     4201 ctgcgggttt actacgtcgc ccagacggac gtggcgcccc cgaccttcac
//
LOCUS       DASWAV010000609         3664 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U2665
            k141_516955, whole genome shotgun sequence.
ACCESSION   DASWAV010000609 DASWAV010000000
VERSION     DASWAV010000609.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960316
            Sequence Read Archive: SRR10260007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 3664)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3664)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:47:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,929
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3664
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_516955"
                     /isolate="SMAG_U2665"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.36444 N 132.31889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..906
                     /locus_tag="VF958_12615"
     CDS             <1..906
                     /locus_tag="VF958_12615"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX9149997.1"
                     /translation="AIVKKLAGVLGGTVSLESSPGKGSRFTVVLPRELPRETPVIAAV
                     AEPPAWPVVEAPRRSVLVVDDDESVRRLLAYELQPLGVAILEAADGKTALEIASTQAP
                     DVILLDVLMPGLDGWETLRSLKENPATRGIPVVVLSVLENRAFGLSLGAFDYLVKPVE
                     MPQLVDTLSRAGVLASRGHLLVVDDDPDVRELLARGCISAGYRVQSVAGGAEALEAMS
                     QDPPSAVILDLMMRPPDGFEVLCRMREDPALRATPVLIVTAKDLTATEYAELSGSAQR
                     VIRKATDPSRLVAEVLRAVQEEKTA"
     gene            911..2926
                     /locus_tag="VF958_12620"
     CDS             911..2926
                     /locus_tag="VF958_12620"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX9149998.1"
                     /translation="MKILVVEDSPDIRVLVRMLLEAGGHEVLTAADGRAGVEAAREVQ
                     PDLVLMDLSLPILSGWEAARAIKDDPATSSIPVVAVTAHAMHGDRERALAAGCDGFIA
                     KPIDEETFEPLVRSFFRPRAEEASPSIGDTAVALPRQAGSYEPGRILVVDDHEEVAAL
                     IKQDLESEGHEVLVARTPDGASGMASDNSPFDLAIVDVMLGADSGYDLTAELVARSPE
                     YLPVLLVTAGEIDREKGYSAGADDFIAKPLNATELVARARSLIRVGRAIRTQGRAARE
                     RSEAYEKLAELDRLKSDFLSTVSHELRTPLNTIILLSHLIEREGKTPSDEERRLHDIR
                     VIRESAETLLRMINNILDLARLEAGQRDLHPQRVPLRPLLEETSDMLEPQARAKGLVL
                     ATEIAPDLPHTAVLDREKVSRVLINLLSNAVKFTQKGRVVLRAAPWQESIVFEVEDTG
                     SGVPAHLVTTVFQPFRQIRTGDGEAPRGSGLGLTISKQLVELMGGDLLFDSREGSGTR
                     VSFTLPQLHDVGLEEPSEPERVAAPRERDAGKGRRILIVEDDSSSRYGLRSLLESEGY
                     EVAEAAALAEADQIVLREPPDVVILDITLPDGDGAEWLRLMTKREGRVPFSVIALTGI
                     TADEDTRRIEQSGVRVVLQKPVNVGLLFQALDDCAAAAGVVTPARPS"
     gene            complement(2904..>3664)
                     /locus_tag="VF958_12625"
     CDS             complement(2904..>3664)
                     /locus_tag="VF958_12625"
                     /inference="COORDINATES: protein motif:HMM:NF024091.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX9149999.1"
                     /translation="QLKWVRFEIARVQYHLDRNMDPEAISGPLFARAFANASSYLLLP
                     LLAILFAADIVSSEFAQGTIKLLLTRPVGRARVLTSKFAALAVAITVTVLLGGVVAYL
                     FGGLAFGYRGWGAPVLTGFRLSGETFDPTAVRAVPLWEDALLAFGLAWYAALTVGAIA
                     FLTSVVLRSTAAAMGTMLAALIAGTILPRVAPSWEAQRYLFVTNLPLPDYYSGSPPPI
                     SGMTLPFSVVVLALWAIAASAVAYGVFVRRDVLA"
BASE COUNT          610 a         1254 c         1240 g          560 t
ORIGIN      
        1 gccatcgtca agaagctcgc gggcgtcctc ggagggacgg tttccctcga gagctccccc
       61 ggcaagggct cccgtttcac ggtcgtcctt ccgcgcgagc ttccgcgcga gacgcccgtg
      121 attgccgccg ttgccgagcc cccggcgtgg ccggtggtcg aggccccacg gcgttcggtc
      181 ctcgtcgtcg acgacgatga gagcgtgcgg cgcctgctcg cgtacgagct ccagccactc
      241 ggcgtcgcca ttctcgaggc cgccgacggc aagacggcgc tcgagatcgc ctccacgcag
      301 gctcccgacg tgatcctcct cgacgtgttg atgccgggac tcgatggctg ggagacgctt
      361 cgcagtctga aagagaatcc cgcgacgcgc ggcattccgg tcgtcgtgct gtccgtcctc
      421 gagaaccgcg cgttcgggct ttctctcggc gccttcgatt atctggtcaa gccggtggag
      481 atgccgcagc tcgtcgatac gctctctcgc gcgggtgtcc tcgcctcgcg cgggcacctt
      541 ctggtcgtgg acgacgatcc cgacgtgcgg gaactcctgg ctcgcggctg catcagcgcg
      601 ggatatcgcg tccagagcgt ggccggcggc gccgaagctc tcgaagcgat gtctcaggat
      661 ccgccgagtg cggtcatcct cgacctgatg atgcgtcctc ccgacgggtt cgaggtcctc
      721 tgccggatgc gtgaagaccc ggcgcttcgg gcgacgccgg tcctgatcgt caccgcgaaa
      781 gacctcacgg cgaccgaata cgccgagctc tccggctcgg cgcaacgggt gatccggaag
      841 gcgacggatc cgtcacgcct cgtcgccgaa gttctccggg cggtccagga agaaaagact
      901 gcgtgaggcg atgaagatcc tcgtcgtcga ggactctccg gacatccgcg tcctcgtgcg
      961 gatgctgctc gaggccggcg ggcacgaggt cctgaccgcc gcggatggac gagccggcgt
     1021 cgaggcagcc cgcgaggtac agcccgacct cgtcctcatg gatctttcgc tgccgatcct
     1081 ttcgggttgg gaggccgcgc gcgcgatcaa ggacgacccc gcgacgtcct ccatcccggt
     1141 cgtcgccgtg accgctcacg cgatgcacgg agatcgcgag cgggcgctcg cggcgggctg
     1201 cgacggattc atcgccaagc cgatcgacga agagacgttc gagccgctcg tccgctcgtt
     1261 ctttcgcccg cgggccgagg aagcgtcccc ctcgatcggg gataccgcag tcgcgcttcc
     1321 ccggcaggcg ggctcgtacg agccaggacg catcctcgtc gtcgacgatc acgaggaggt
     1381 tgccgcgctc atcaagcagg acctcgaaag cgaaggtcac gaagtcctcg tcgcgcggac
     1441 gcccgacgga gcgtcgggga tggcgagcga caacagcccg ttcgacctcg cgatcgtcga
     1501 cgtcatgctc ggggccgatt ccggctacga cctcacggcg gaactcgtcg ctcgttcgcc
     1561 cgagtacctc ccggtgctcc tcgtcacggc cggcgagatc gatcgggaga agggctattc
     1621 cgccggcgcg gacgacttca tcgcgaagcc gctcaacgcc acggagctcg tcgcccgcgc
     1681 ccgctcgctc attcgcgtcg gccgtgcgat ccggacccag ggtcgggccg cgcgggagcg
     1741 ctccgaggcg tacgagaaac tcgcggaact cgaccgcctg aaatccgact tcctttccac
     1801 ggtttcgcac gagctgcgca cgcccctgaa cacgatcatt ctcctttcac atttgatcga
     1861 gcgcgagggg aagaccccct ccgacgagga gcggcgcctt cacgacatcc gtgtcatccg
     1921 cgagagcgcg gagacgctgc tccggatgat caacaacatc ctcgatctcg cgaggctcga
     1981 ggcgggacag cgcgacctcc acccgcagcg cgtgccgctt cggcccctgc tcgaggagac
     2041 ctccgacatg ctcgagcccc aggcgcgtgc gaagggcctc gtcctcgcga ccgaaatcgc
     2101 accggatctc ccgcacaccg cggttctcga ccgcgagaag gtttcgcgcg tgctgatcaa
     2161 ccttctctcg aacgccgtca agttcaccca gaaagggcgc gtcgtccttc gcgccgcgcc
     2221 gtggcaggag tccatcgtct tcgaggtcga ggacacgggg agcggagtgc cggcccacct
     2281 cgtcacgacg gtattccagc ccttccgtca gatccggacc ggcgacggag aagcgccgcg
     2341 cggatccgga ctcgggctga cgatttcgaa gcagctcgtg gagctcatgg gaggcgacct
     2401 ccttttcgac agccgggagg ggtccgggac gcgcgtttcg ttcacgctcc cccagttgca
     2461 cgatgtgggg ctcgaggagc cgtccgaacc cgagcgcgtc gcggctccgc gggaacgcga
     2521 cgccgggaaa ggccggcgga ttctcatcgt tgaggacgac tcctcctcga ggtacgggtt
     2581 gaggagcctt ctcgaatccg aggggtacga ggtcgcagag gcggccgcgt tggccgaggc
     2641 cgatcagatc gtattgcggg agcctccgga cgtggtcatt ctcgacatca cgcttcccga
     2701 cggcgacggc gccgagtggc tccggctcat gacgaagagg gaggggcgcg tccccttctc
     2761 cgtcatcgcg ctgaccggca tcacggccga cgaagacacg aggcgcatcg aacagtccgg
     2821 cgtgcgtgtt gtcttgcaga agcccgtcaa cgtcgggctc ctcttccagg cgctcgacga
     2881 ctgcgcggct gcggcgggag tggttacgcc agcacgtccc tcctgacgaa gacgccgtag
     2941 gcgaccgcgg acgcggcgat ggcccacagt gccagcacca cgacggaaaa cggaagcgtc
     3001 atcccggaga tcggcggcgg cgagccggag tagtagtcgg gcagcggcag gttcgtcacg
     3061 aaaagatagc gctgcgcctc ccacgaggga gcgacccgcg gcaggatcgt gccggcgatc
     3121 agcgccgcga gcatcgtccc catggccgct gcggtcgagc gcagcacgac cgacgtcagg
     3181 aaggcgatcg caccgacggt cagtgccgcg taccaggcga ggccgaacgc gaggagcgcg
     3241 tcctcccaga gcggaaccgc gcgcacggcg gtcggatcga aggtctcgcc ggacagccgg
     3301 aaaccggtca ggacgggcgc gccccaccct cgatacccga acgccaggcc tccgaagagg
     3361 taagcgacga cgccgcccag gaggaccgtc acggtgatcg cgaccgcgag ggcggcgaac
     3421 ttcgaggtga gaacccgcgc gcgcccgacc ggccgcgtga gcaggagttt gatcgttccc
     3481 tgcgcgaact cggaggacac gatgtcggcg gcgaagagga tcgccaggag aggcaggaga
     3541 aggtaactcg acgcgttcgc gaacgcgcgc gcgaagagcg gaccggagat cgcctccggg
     3601 tccatgttgc gatcgaggtg gtactgcacg cgggcgatct cgaaccggac ccacttcaac
     3661 tgcg
//
LOCUS       DASWAV010000610         2182 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U2665
            k141_276269, whole genome shotgun sequence.
ACCESSION   DASWAV010000610 DASWAV010000000
VERSION     DASWAV010000610.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960316
            Sequence Read Archive: SRR10260007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2182)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2182)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:47:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,929
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2182
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_276269"
                     /isolate="SMAG_U2665"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.36444 N 132.31889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1992
                     /locus_tag="VF958_12630"
     CDS             <1..1992
                     /locus_tag="VF958_12630"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HEX9150000.1"
                     /translation="ETPHRSGSVIGGRYRIEQVLGFGGMGVVYRARDLKLESEIALKR
                     IRPDRFAPERRETLRREIILARKVTHENVCRVYDLVEVDGEEFVSMEYLPGRTLKDIE
                     TKERILPLGRGLAIAKGMCSGLAAAHRIGVLHRDLKPENVMVGEDGKPRLMDFGIAVE
                     SGRFVPQGETVPGTPQFLAPELLQGAAPSVRTDVYAMGVVLYEMFTGRVPIDDDNTAR
                     LVRRIVREAPPTVRSLRPDLPPELASILDRAIAKDPEARFSDASALADAIAAFEGQVL
                     DRVLAEVSVTRAKMIKLMVILEANKSLAATFDPTETLRIILRTATSETDAERGTIFLK
                     DPKTGELVSQILEGGSIAPIRLPSGRGIAGSVAATGDIVNVGDAYNDRRFDSRTDAGS
                     GFHTVSILAAPLRTPAGEIVGVVEILNKRRGPFTKEDEEFLAEVGTHAALAVAGDRQH
                     EAAVEVARREGAARALRGASAFLVPHAWPETPGFESAPLRWSSDELNLLGFAVDAAPG
                     RVAYLLLEPSGEPEDSLASLLRALEAGRRLLPEATPEEIVGAIGALGTDCAVAAARWE
                     GERVSVAGAGGAAAPALFRAGRPLPPGSAAARGDLLVVASSGLGRLRPAPAAPLERFA
                     WLAGGQPLPAAFARLVADFKREGVSAGDRDVLLLAARRA"
     gene            1989..>2182
                     /locus_tag="VF958_12635"
     CDS             1989..>2182
                     /locus_tag="VF958_12635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009762620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis protein"
                     /protein_id="HEX9150001.1"
                     /translation="MTLRELRYVLVDVFADRPLAGNPLAVFPDAGGLDDATLQLVARE
                     MDHAATSFLSPAAAPDAAFR"
BASE COUNT          308 a          780 c          771 g          323 t
ORIGIN      
        1 gagacccccc accggtcggg ctccgtcatc ggcggccgct accgcatcga gcaggtcctc
       61 ggtttcggcg gcatgggagt cgtctatcgc gcgcgcgacc tgaagctcga gtcggagatc
      121 gcgttgaaac gcatccggcc cgaccggttc gcgccggagc ggcgggagac cctgcgccgc
      181 gaaatcatcc tggcccgcaa ggtgacccac gagaacgtgt gtcgcgtgta cgacctcgtg
      241 gaagtcgacg gcgaagaatt cgtctcgatg gagtatctgc ccggtcgcac gctgaaggac
      301 atcgagacga aagaaagaat ccttccgctc ggtcgcggcc tcgccatcgc caagggaatg
      361 tgcagcgggc tcgcggcggc tcaccgcatc ggcgtcctgc atcgcgactt gaagccggag
      421 aacgtgatgg tcggggagga cggcaagccg cggctgatgg acttcgggat cgccgtcgag
      481 agcggtcgat tcgttccgca gggcgagacc gtccccggga cgccgcagtt tctcgcgccg
      541 gagctcctcc agggcgccgc cccgagcgtg cggacggacg tttacgcgat gggggtcgtt
      601 ctctacgaga tgttcacggg gcgcgtgccg atcgacgacg acaatacggc gcggctcgtg
      661 cggcggatcg tccgggaggc gccgccgacg gtccggagcc tccggccgga tctcccgccc
      721 gagctcgcga gcatcctcga ccgcgcgatc gcgaaggacc cggaggcgcg gttctccgac
      781 gcctcggcgc tcgccgacgc gatcgccgcc ttcgaaggac aggttctcga tcgcgtcctg
      841 gcggaggttt ccgtcacgcg cgccaagatg atcaagctca tggtgatcct cgaggcgaac
      901 aagtcgctcg cggcgacgtt cgatccgacc gagacgctgc ggatcatcct ccgcacggcc
      961 acgagcgaga ccgacgcgga gcgcgggacg atcttcctga aggatccgaa gacgggagag
     1021 ctcgtcagcc agatcctcga aggaggatcc atcgccccga tccgtcttcc gtcgggacgc
     1081 gggatcgcgg gctccgtggc ggcgaccggc gacatcgtca atgtcggcga cgcctacaac
     1141 gaccgcaggt tcgactcccg gaccgacgcg gggtccggct tccacaccgt ctcgatcctc
     1201 gcggcgcccc tgcggacgcc agcaggtgag atcgtcgggg tcgtcgaaat cctcaacaag
     1261 cggcggggcc ctttcacgaa ggaggacgaa gagttcctcg ccgaggtggg cacgcacgcg
     1321 gcgctcgccg tcgcgggcga ccgacagcac gaagcggcgg tcgaggttgc gcggcgcgaa
     1381 ggcgcggcga gggcgttgcg cggcgcgtcg gcgttcctcg ttccgcacgc ctggcccgag
     1441 acgccgggct tcgaatcggc gcccctgcgc tggagctcgg acgagttgaa tctcctcggc
     1501 ttcgcggtcg acgctgcacc cggccgcgtc gcctacctcc tcctcgagcc ctccggcgaa
     1561 ccggaagact cgctcgcgtc gctcctccgc gcgctcgaag cggggcgccg gctgcttccg
     1621 gaagccacgc ccgaggagat cgtgggcgcc attggcgccc tcgggaccga ctgcgcagtc
     1681 gccgcggcgc gctgggaggg agagcgcgtc tcggtcgccg gggccggagg cgcagccgcg
     1741 ccagcgctct tccgggcggg gcggccgctg ccgcccggaa gcgccgcggc ccggggcgat
     1801 ctcctcgtcg tcgcttcgag cgggctcggc cggcttcgtc cagcgcctgc ggctcccctc
     1861 gagcgcttcg cgtggctcgc gggggggcaa ccgttgcccg ccgcattcgc ccggctcgtc
     1921 gccgacttca agcgggaggg cgtgtcggcc ggagaccgtg acgtgctcct cctcgccgcg
     1981 cggcgagcgt gaccctccgg gagcttcgct acgtcctcgt ggacgtcttc gccgaccggc
     2041 ccctcgccgg aaaccctctc gcagtcttcc ccgacgccgg cgggctcgac gacgccaccc
     2101 tccagctcgt cgcgcgcgag atggaccatg ccgcgacgtc gtttctctcc ccggcggcgg
     2161 cgcccgacgc cgcgttccgg tt
//
LOCUS       DASWAV010000611         7599 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U2665
            k141_235835, whole genome shotgun sequence.
ACCESSION   DASWAV010000611 DASWAV010000000
VERSION     DASWAV010000611.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960316
            Sequence Read Archive: SRR10260007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 7599)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7599)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:47:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,929
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7599
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_235835"
                     /isolate="SMAG_U2665"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.36444 N 132.31889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1621)
                     /locus_tag="VF958_12640"
     CDS             complement(<1..1621)
                     /locus_tag="VF958_12640"
                     /inference="COORDINATES: protein motif:HMM:NF017400.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIVB peptidase S55 domain-containing protein"
                     /protein_id="HEX9150002.1"
                     /translation="MKSALFAVAVSVGSIVSSASLGAAPSPATAAAAAALPGDRPAEI
                     FPLEKIVPGLKGYGFSDLGGGKGIQRFDVEMIGTLRRYAPKQDLILAKVSGAGLEHSG
                     IIAGMSGSPIYVDGKLVGALAYGWPFSKDPICGITPIQSMLDIRHAPAAAPVPIGGPA
                     ARASQLVSMFASHRFDGALDSLLAPFRQSDAAGAMSRLPLPVSFAGAAGGDAGWLFGR
                     VAEAAGWVAAPAGSSGQSPKSASDETTSPVQPGSSLATVLLSGDMLLAATGTATWVEG
                     DSVLAFGHPFLSMGPIDMPMARAEVLTVLPSVYRSFKFADTGPVFGSVTQDRSTGILG
                     TVGSRARMVPITVRISSDELPVQTYHFEAVHNSMLTPVLTAMAIDSVLTTLEKRAGER
                     TLVWKSSIRTPDRNVRWDTVFTGLAAREDAVTSLALLTNYLMANEFHDLPILGVDVEI
                     AHSDRLQSGRIVNVEPARERFRAGERVPVRVDVVDFRGGLRRVMLELDVPSDTPPGPL
                     TVFVGDGNSATAYDLAVYPADPQSLDQVLDFL"
     gene            1709..2476
                     /locus_tag="VF958_12645"
     CDS             1709..2476
                     /locus_tag="VF958_12645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006000508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HEX9150003.1"
                     /translation="MLRSISMISAGFQAVSAENIGQGQGILSFFKETGPTAKVVLGIL
                     LFFSLVSWIIIFAKFVRLRRVSRQSEKFVEFFRKSRRFSEVNTVANQLTETPLSTLFK
                     AGYAELDAQVKANRPDDPGATASGVTMSQRFLIRNISGIERALERAIGVELSRLTKYM
                     TFLATTAAACPFIGLFGTVWGIMQSFRAIGTTGSTSIAAVAPGISEALVNTAAGLAAA
                     IPALIFYNYFMGKVREQRAGMEDFSLEFINLAERNFT"
     gene            2588..3025
                     /locus_tag="VF958_12650"
     CDS             2588..3025
                     /locus_tag="VF958_12650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004513694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ExbD/TolR family protein"
                     /protein_id="HEX9150004.1"
                     /translation="MAFQRQSGDPFDHLADINVTPLVDVMLVLLIIFMVTAPMLHQGV
                     SVALPKTATTNLPATMEDPVMLSITKDGLYYINETPVARGLLRDRLERFLRGRKDRFV
                     LLKADRSLSYGTVVETLDLLNRMGIEGLGMVTDTTGTRAAARP"
     gene            3041..3784
                     /locus_tag="VF958_12655"
     CDS             3041..3784
                     /locus_tag="VF958_12655"
                     /inference="COORDINATES: protein motif:HMM:NF024503.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="HEX9150005.1"
                     /translation="MDTVGEIIWERGKERFPLGVGTALAVVLHLAIAAAFVYSSMVHP
                     VRFISPRAVAVRLLPAGSLKGGEAPAPAPAPPPETKKILKPAPEDEPPPPTDKAVLLP
                     EKEDKKKAATAPRPAPDRPKAPEVSLPSSGGSPGGGAGGAVGAGGNVGVSGAAFDSDF
                     QFSYYVERMLVAIGMNWFKPSQGGTVSPVVHFKIEKDGTVSDASVERSSGLPFVDRAA
                     LRAVLSSSPLPPLPAEYTGSQLGVHLKFD"
     gene            3843..5147
                     /gene="tolB"
                     /locus_tag="VF958_12660"
     CDS             3843..5147
                     /gene="tolB"
                     /locus_tag="VF958_12660"
                     /inference="COORDINATES: protein motif:HMM:TIGR02800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Tol-Pal system beta propeller repeat protein
                     TolB"
                     /protein_id="HEX9150006.1"
                     /translation="MRRTLALLILSAAPFALASFARGQTAQNPQNQDLWISINKPAAR
                     KLAIAVPELTTAGTLKSTISDPFTGTLRSDLEYSGAFVIADPAHYPTGFRDPTTPDAS
                     DHWRAGGGEVLVDTRAEVSGDRVSVEARVWDLTSRKMIMGKRYSGGATYVERIAHTLS
                     NDIVKYFTGKDGLFLTSIVFVSDRDGSKDIYAMDFDGRSVRRLTSVRSLAINPSGGRS
                     GKIVYTSYAKLFPQIWVMASDGGGKREVSTGVELNASPSFSPDETQIAFAGSAKGNTD
                     IYVVNAGGGGLRRLTTTRAIEASPAWSPTGRQILYTSDQSGNPQIYVVDPEGTGARRV
                     TFAGNWNDEASWSPDGARIAFACRNEGDFNICLMDFATGQTVQLTSEGSNGHPTWSPD
                     GQKIVYSSRRGGRTNVYSMDINGQNKRQLTDSGNNIQPVWLP"
     gene            5249..5815
                     /gene="pal"
                     /locus_tag="VF958_12665"
     CDS             5249..5815
                     /gene="pal"
                     /locus_tag="VF958_12665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015459120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-associated lipoprotein Pal"
                     /protein_id="HEX9150007.1"
                     /translation="MKRHSTTIVVLAFVAAAILPMAQGCKKKPPTTTAEARPPVEAPA
                     PPETRVPPPPAAAPRDVEVGDVMATDIAELNTKGYLKDAYFDYDQSDLREDARGSLSG
                     NAEWLKRYPSIQVLIEGHCDERGTSAYNLALGDRRANAARDYLDSLGIASSRIRTVSY
                     GKERPACTESSESCWQQNRRAHFVITSK"
     gene            5817..6716
                     /gene="ybgF"
                     /locus_tag="VF958_12670"
     CDS             5817..6716
                     /gene="ybgF"
                     /locus_tag="VF958_12670"
                     /inference="COORDINATES: protein motif:HMM:NF024915.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tol-pal system protein YbgF"
                     /protein_id="HEX9150008.1"
                     /translation="MKSRYRFPLLAAAAVAAATGAVGCVSSDDIQGLHRQMNDIESQI
                     QALERKSSSKEEVARLNESVAGQTQQLLKSNADTGVKLDELSNEIERLQGKLEDTNRR
                     LSQLSQQIAETQGDLLRMRGGSAPAPSGTVLPPGAPPTSPSTNPSSSRVGEPMANTQS
                     PPATESVVRPAAGPAELYDTAYADYTKARYALAIQGFQEYLGTYPNTDLSDNAQYWIG
                     ESHYAQKKYRDAIVDFDRLLKDFPKSDKAPAALLKKGYALLELGQKAESVVSLQYVIH
                     EYPSSEEARLARARLKSIGVEAR"
     gene            6730..6987
                     /gene="rpsT"
                     /locus_tag="VF958_12675"
     CDS             6730..6987
                     /gene="rpsT"
                     /locus_tag="VF958_12675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002645194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HEX9150009.1"
                     /translation="MANIKSAKKRILQGEKRRARNKSARSRLRSAIKKHRSSEDASKS
                     QGLPATYSEIDRAEKRSAIHRNAAARYKSRLAKAAGGKPKA"
     gene            complement(6998..7519)
                     /gene="lptE"
                     /locus_tag="VF958_12680"
     CDS             complement(6998..7519)
                     /gene="lptE"
                     /locus_tag="VF958_12680"
                     /inference="COORDINATES: protein motif:HMM:NF016290.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0043165 - Gram-negative-bacterium-type cell
                     outer membrane assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly lipoprotein LptE"
                     /protein_id="HEX9150010.1"
                     /translation="MNPRAAVAALLLAAAAGSGCGYSLVGHGSTLPPTVRVIRFRTLA
                     NETSRVGVEQRVSREVARELATRGRFEVSAGAGRADAELAGAVTQFDLYPVAFDEAGR
                     AKEYQVAVTARVTLKQLPDEKTLWENPAYTFRESYTLPASAASYVDRENEAIDRVAER
                     FAASLVSSLLEGF"
BASE COUNT         1348 a         2618 c         2474 g         1159 t
ORIGIN      
        1 agaggaaatc gaggacctga tcgagcgact gcgggtctgc cgggtaaacg gcgaggtcgt
       61 acgcggtcgc cgagtttccg tcaccgacga acacggtcaa cgggccgggc ggcgtgtcgc
      121 tgggaacgtc gagctcgagc atgactcggc ggagtccgcc gcggaagtcc acgacgtcga
      181 cgcgcaccgg cacccgctcg cccgcccgga agcgctcccg ggccggctcg acgttgacga
      241 tgcggccgct ctgcaaccgg tcggagtgcg cgatctcgac gtcgacgccg aggatcggaa
      301 ggtcgtggaa ttcgttcgcc atgaggtagt tcgtcaggag cgcgagcgac gtgaccgcgt
      361 cttcgcgcgc ggcgaggccg gtgaagacgg tgtcccagcg gacgttgcgg tccggagtcc
      421 ggatcgagga cttccacacg agcgtccgct ctcctgcgcg cttttcgagg gtcgtcagga
      481 cgctgtcgat cgccatcgcc gtgaggaccg gcgtcaacat cgagttgtgg acggcctcga
      541 agtggtacgt ctggaccggg agctcgtccg aagagatccg gacggtgatg ggcaccatcc
      601 tcgcgcgcga tccgaccgtc ccgaggatgc cggtcgatcg gtcctgcgtg accgatccga
      661 agacgggccc cgtgtccgcg aacttgaacg accggtaaac cgacggcaga acggtcaaca
      721 cctcggcgcg cgccatcggc atgtcgatcg gtcccatcga caggaacggg tgcccgaagg
      781 cgagaaccga gtcgccctcc acccaggtcg ccgtgccggt cgcggcgagc agcatgtctc
      841 cggacagaag gaccgtcgcg agcgacgatc cgggctggac cggagaagtc gtctcgtccg
      901 aggcggactt cggactctga ccggaagaac ccgccggcgc ggcgacccat cccgccgctt
      961 ccgcgacgcg tccgaagagc cagcccgcgt caccgccggc agcgccggcg aaggacacgg
     1021 ggagcgggag gcgcgacatc gcgcccgccg cgtcggattg gcgaaacggc gcgagcagcg
     1081 agtcgagcgc tccgtcgaat cggtgagacg cgaacatcga aacgagttga cttgcccgcg
     1141 ccgccggtcc gccgatcggc acgggagcgg cggccggcgc gtgacggatg tcgagcatgc
     1201 tctggatcgg cgtgatgccg cagatcgggt ccttcgagaa gggccaaccg tacgcgaggg
     1261 cgccgacgag cttgccgtcc acgtagatcg ggctcccgct catcccggcg atgatcccgg
     1321 aatgctcgag ccccgcgccc gaaaccttcg cgaggatcag gtcctgcttc ggagcgtacc
     1381 gccggagcgt cccgatcatc tcgacgtcga agcgctggat tcccttgccg ccgccgagat
     1441 ccgagaagcc gtaccccttc aggccgggga cgatcttctc gagcggaaag atctcggccg
     1501 gccggtcgcc cggcagagcc gcggccgcgg ccgcggtcgc gggagacggc gcggcgccga
     1561 gcgatgcgct cgaaacgatc gaaccgaccg aaacagcgac ggcgaaaagc gcggatttca
     1621 cgccgagaac aacgcctccc ctcccgatct ccattccact tcgcctgagc tcccgacagc
     1681 tttcactgct gacagctgac agttcactat gttacgttcg atctcgatga tctccgccgg
     1741 ttttcaggcc gtctcggcag aaaacatcgg ccagggacag gggatcctct ccttcttcaa
     1801 agagaccggc ccgaccgcca aggtcgttct cggcatcctc ctcttcttct cgctcgtctc
     1861 ctggatcatc atcttcgcca agttcgtccg cctgcggagg gtttcccgcc agtcggagaa
     1921 gttcgtcgag ttcttccgga agtcccgccg attctccgaa gtgaacacgg tggccaacca
     1981 gctcacggag accccgctct cgaccctgtt caaggccggc tacgcggagc tcgacgccca
     2041 ggtgaaggcg aaccggccgg acgaccccgg cgccacggcg agcggggtca cgatgagcca
     2101 gcggttcctg atccggaaca tctcggggat cgagcgggcg ctcgaacgcg cgatcggggt
     2161 cgagctctcc cgactcacga agtacatgac gttcctcgcg acgacagcgg cggcgtgccc
     2221 gttcatcggg ctcttcggga ccgtgtgggg gatcatgcag tcgttccgtg ccatcggcac
     2281 gaccgggtcc acgtcgatcg cggccgtcgc gcccggaatc tccgaggccc tcgtcaacac
     2341 ggcggccggc ctcgcagcgg cgatcccggc gctcatcttc tacaactact tcatgggaaa
     2401 ggtccgcgag cagcgggcgg gaatggagga cttctccctc gagttcatca acctggcgga
     2461 gcggaacttc acctaaagaa atgcggggtc aggtcttcac gagtcgcgcc ggcattgagt
     2521 tgaccagact gcccttgcca catcgcggcg cgatgtggca agtggaggcc tgacccccgg
     2581 gcaacccatg gcatttcaac gccaatccgg cgaccctttc gatcacctgg cggacatcaa
     2641 cgtcacgccg ctcgtcgacg tcatgctcgt cctgctcatc atcttcatgg tgaccgcgcc
     2701 gatgctccac cagggcgtgt cggtcgctct gccgaagacg gcgacgacaa acctgccggc
     2761 caccatggaa gaccccgtca tgctctcgat caccaaggac gggctctact acatcaacga
     2821 gacgccggtc gcccgcggtc tcctgcgcga ccgtctcgaa cggttcctgc gcggccgcaa
     2881 ggaccgtttc gtcctgctga aggccgatcg cagcctctcg tacggcaccg tcgtcgagac
     2941 cctcgacctc ctgaaccgga tgggaatcga aggcctggga atggtcacgg acacgaccgg
     3001 cacgagagcc gcggccaggc cgtagccggg gcgccggatc gtggacaccg tcggcgagat
     3061 catctgggag cgcgggaagg aacgcttccc gctcggtgtc ggcaccgctc tcgcggtcgt
     3121 tctccatctt gcgatcgcgg cggcgttcgt ctactcctcg atggtccatc ccgtgaggtt
     3181 catctccccg cgcgcggtcg ccgtccggct tctgcccgcc gggtcgctca agggaggcga
     3241 ggcgccggcg cccgctcccg cgcctccccc cgagacgaaa aagatcctga agccggcgcc
     3301 cgaggacgaa ccgcccccgc cgaccgacaa ggccgtgctc ctccccgaga aggaggacaa
     3361 gaagaaggcg gcgacggccc cgcggccggc gcccgaccgc ccgaaagcgc ctgaggtgag
     3421 ccttccgtcg tccggaggga gtcccggcgg aggcgcgggt ggagcggtcg gcgccggggg
     3481 aaacgtcggc gtctcggggg ccgcgttcga ctccgacttc cagttctcct actacgtcga
     3541 gcggatgctc gtcgcgatcg gcatgaactg gttcaaaccc tcgcagggag gcaccgtctc
     3601 gccggtcgtc cacttcaaga tcgaaaagga cgggacggtc tccgacgcga gcgtcgagcg
     3661 ctcgagcgga ctgcccttcg tcgaccgcgc cgcgctgcgg gcggtgcttt cgtcctctcc
     3721 gctcccgccg cttccggccg agtacaccgg cagccagctc ggcgtccacc tgaagttcga
     3781 ctgaatcctc agattcgaca aatcggacaa atcctctatc ctcactccga atggagaccc
     3841 ggatgcgccg aacgctcgcc cttctgatcc tttctgccgc gcccttcgcg ctcgcctcgt
     3901 tcgcgagagg acagacggcc cagaacccgc agaaccagga cctctggatt tcgatcaaca
     3961 agccggcggc gcgcaagctc gcgatcgccg tccccgagct caccacggcc gggacgttga
     4021 aatcgacgat ttccgacccc tttacgggga cgctgcgctc ggacctcgaa tactcggggg
     4081 ccttcgtcat cgccgacccg gcgcactacc cgacgggctt ccgcgacccc acgactccgg
     4141 acgcttccga tcactggcga gccgggggcg gtgaggtgct cgtcgacacg cgcgcggaag
     4201 tctccggcga tcgggtgtcg gtcgaagcgc gggtatggga cctcacctcg cgcaagatga
     4261 tcatgggcaa gcgctactcc ggcggagcga cgtacgtcga gcgaatcgcg cacacgctct
     4321 cgaacgacat cgtgaagtac ttcaccggca aggacggcct ctttctgacg tccatcgtct
     4381 tcgtttcgga tcgcgacggc tcgaaggaca tctacgcgat ggatttcgac gggcgcagcg
     4441 tccggaggct cacgtccgtc cggtcgctcg cgatcaaccc ctccgggggc cgctccggca
     4501 agatcgtgta cacgagctac gcgaagctct tcccgcagat ctgggtcatg gcctccgacg
     4561 gcggggggaa gcgggaggta tcgaccgggg tcgagctgaa cgcgtctccg tcgttctccc
     4621 ccgacgagac ccagatcgcg ttcgccggct cggcgaaggg gaacaccgac atctacgtcg
     4681 tcaacgccgg cggcgggggg cttcggcggc tgacgacgac ccgcgcgatc gaggcttccc
     4741 cggcgtggtc gccgacggga aggcagatcc tctacacgtc ggaccagtcg ggcaacccgc
     4801 agatttacgt cgtggacccg gagggaacgg gagcgcgccg cgtgacgttc gccggcaact
     4861 ggaacgacga ggcttcctgg tcgcccgacg gcgcccggat cgcattcgcc tgccggaacg
     4921 agggggactt caacatctgt ctcatggact tcgcgaccgg gcaaaccgtg caactgacgt
     4981 cggagggaag taacggtcac ccgacgtggt cacccgacgg acagaagatc gtctactcct
     5041 cgcgccgcgg aggccgcacg aacgtctatt cgatggacat caacggacag aacaagcggc
     5101 agctcaccga ctcggggaac aatattcagc cggtctggtt gccgtaaggc gtcgagggtt
     5161 gggcttttcg agggtcgttt gctatattcg gcaacggcgc cccatccggt tttttcggcg
     5221 caaacctgct gatggacaag gagaaggtat gaagaggcac tcgacaacga tcgtcgtgct
     5281 cgcgttcgtc gcggccgcga tactccccat ggcccagggc tgcaagaaga agcccccgac
     5341 gacgacggcc gaagcccgtc ccccggtcga ggcgcccgct cccccggaga cccgcgttcc
     5401 cccgccaccg gctgcggccc cccgggacgt cgaagtcggc gacgtgatgg cgacggacat
     5461 cgcggagctc aacacgaagg gctacctgaa ggacgcgtac ttcgactacg accagtccga
     5521 cctgcgcgag gacgcgcgcg gctcgctctc cggcaacgcg gagtggctga agcgctatcc
     5581 gtcgatccag gtcttgatcg agggacattg cgacgagcgc ggaacctccg cctacaacct
     5641 ggcgctcggc gaccggcgcg ccaacgcggc gcgcgactat ctcgactcgc tcggcatcgc
     5701 ctcgtcgcga atccgcaccg tgtcctacgg gaaggagcgg ccggcctgca ccgagtcgag
     5761 cgagagctgc tggcagcaga atcgccgcgc gcacttcgtc atcacctcca agtaacgtga
     5821 aatcgcgata ccgctttccc cttcttgctg cggccgcggt cgccgcggcg accggcgccg
     5881 tcgggtgcgt ctcctccgac gacatccagg ggctgcaccg ccagatgaac gacatcgagt
     5941 cgcagatcca ggctctcgag cgcaagtctt cgtcgaagga agaggtcgcg aggttgaacg
     6001 agagcgttgc ggggcagacg cagcaactcc tgaaatcgaa cgccgacacc ggcgtcaagt
     6061 tagacgagct ttcgaacgag atcgagcggc tccaggggaa gctcgaggac actaaccggc
     6121 gcctctcgca gctgtctcag cagatcgcgg aaacccaggg cgacctgctc cggatgcgcg
     6181 gggggagcgc cccggcgccc tcggggaccg tcctgccgcc cggcgctcct ccgacttctc
     6241 cctcgaccaa cccctcctcg tcccgggtcg gcgagccgat ggcgaacacc cagagcccgc
     6301 ccgccaccga atccgtcgtt cggccggcgg cgggtcccgc ggagctctac gacacggcgt
     6361 acgccgacta cacgaaggcc cggtacgcgc tcgcgatcca gggtttccag gagtacctcg
     6421 gaacgtatcc aaacacggac ctctccgaca acgcgcagta ctggatcggt gagtcgcact
     6481 acgcgcagaa gaagtaccgg gacgcgatcg tcgacttcga ccggttgctc aaggacttcc
     6541 cgaaaagcga caaggcgccc gcggctctct tgaagaaggg ctacgccttg ctcgagctcg
     6601 gacagaaggc cgagtccgtc gtgtctctcc agtacgtaat ccacgaatac ccctcctccg
     6661 aggaggcgcg gctcgcgcgc gcgcggctga agtcgatcgg ggtcgaagct cgataggagt
     6721 caagtctcga tggccaacat caagtccgcc aagaaacgaa tcctccaggg ggagaagcgc
     6781 cgcgcccgga acaagagcgc acggtcgcgc ctccgttcgg cgatcaagaa gcaccgcagc
     6841 tccgaggacg cctcgaagtc ccagggtctc ccggcgacgt actcggagat cgaccgcgcc
     6901 gagaagagaa gcgcgatcca ccgcaatgct gcggcgcggt acaagtcgcg tctggcgaag
     6961 gcggcgggcg gcaagcccaa agcctgagtt ccgcctccta gaacccctcg agcagactgg
     7021 acaccaggct cgcggcgaac cgttccgcga cgcggtcgat cgcctcgttc tcgcgatcga
     7081 cgtaggaagc ggcactcgcg ggaagcgtgt agctctcgcg gaacgtgtag gcggggttct
     7141 cccagagcgt cttctcgtcc gggagctgct tcaacgtcac ccgcgcagtc accgcgacct
     7201 ggtattcctt cgcccggccg gcctcgtcga aggcgaccgg gtagaggtcg aactgcgtca
     7261 ccgcgccggc gagctccgcg tcggcgcggc cggcgcccgc gctcacctcg aagcgcccgc
     7321 gcgtggcgag ctcgcgggcg acctcccggg acacgcgctg ctcgacgccc acccgcgacg
     7381 tctcgttcgc gagcgtccgg aaacggatga cgcggaccgt cggaggaagc gtcgaaccgt
     7441 ggccgacgag cgagtaaccg catccgcttc cggcggcggc ggccaggagg agcgctgcga
     7501 ccgcggcgcg agggttcaac ggacgacgag gttcaagagc cggtccggca cgtagaccga
     7561 gcgggcgatc tccttgccct cgacccacgg acggatcct
//
LOCUS       DASWAV010000612         3226 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U2665
            k141_276373, whole genome shotgun sequence.
ACCESSION   DASWAV010000612 DASWAV010000000
VERSION     DASWAV010000612.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960316
            Sequence Read Archive: SRR10260007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 3226)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3226)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:47:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,929
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3226
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_276373"
                     /isolate="SMAG_U2665"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.36444 N 132.31889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..304)
                     /locus_tag="VF958_12685"
     CDS             complement(<1..304)
                     /locus_tag="VF958_12685"
                     /inference="COORDINATES: protein motif:HMM:NF014909.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HEX9150011.1"
                     /translation="MTGEPGPIGPADDPISNVLASFAEEAARGVASRSEFEALKGRYL
                     GREKGLVPALFARMREIPPPDRAAYGARANAAKREVEEGIARLGESLAHEEARRREL"
     gene            complement(301..663)
                     /gene="rplT"
                     /locus_tag="VF958_12690"
     CDS             complement(301..663)
                     /gene="rplT"
                     /locus_tag="VF958_12690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012635506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HEX9150012.1"
                     /translation="MPRVKRGPKRKNRRAKTLALAKGYYGTKSKSYRMAKLAVEKSLL
                     YAYRDRKARKRDFRGLWIVRINAAARENEISYSKLMDGLKKAGNDINRKMLADLAVTD
                     PPAFTHLVGVAKQALGGA"
     gene            complement(763..960)
                     /gene="rpmI"
                     /locus_tag="VF958_12695"
     CDS             complement(763..960)
                     /gene="rpmI"
                     /locus_tag="VF958_12695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004696338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HEX9150013.1"
                     /translation="MPKLKTHRGAAKRFRRTASGRFKRAKAYKSHILTKKAPGRKRGL
                     AQSTSVSASDHKRVKEMLPYA"
     gene            complement(978..1517)
                     /gene="infC"
                     /locus_tag="VF958_12700"
     CDS             complement(978..1517)
                     /gene="infC"
                     /locus_tag="VF958_12700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013029865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HEX9150014.1"
                     /translation="MDKKSIRINEQIRWKEVRLIGAEGEPLGIVSMADALQKAKDAAL
                     DLVEIAPTANPPVCRIMNFGKFIYQQKKKQQDSKKKQKTTQVKEVKFRPNIDDHDYDF
                     KIKNILKFLAHGDKVKATVQFRGREMARRENGQKVLDRLLGDLAGKAVAESRSEMLGN
                     RLYQILAPVKVHEKAARGA"
     gene            complement(1510..>3226)
                     /gene="thrS"
                     /locus_tag="VF958_12705"
     CDS             complement(1510..>3226)
                     /gene="thrS"
                     /locus_tag="VF958_12705"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005038473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HEX9150015.1"
                     /translation="STAHLTAQAVKRLFPDVQIGIGPPIENGYYYDFLPQRPFTPEDL
                     DEISAEMRRIIGEDLPITRSEMPVSEAISIFERQNDPLKVQIARELPDEFVSCYRQGE
                     FIDLCRGPHVASTGRLGVFKLTHTAGAYWKGDEKNPMLQRIYGASFLTQKELDEYLKR
                     LEDAKARDHRKLGRELELFTFHPLASASPFFLPKGAVLYNGLLAYLREEYRRRGYDEV
                     ITPQIYDAELFKISGHYDNYRDNMYFTEIEEREFGVKPMNCPGHHLLFKERLWSYREL
                     PVRYADFGRLHRYERSGVTAGLTRVRTFSQDDSHIYCPIGEIEPEIARFVEFLDDVYR
                     TFDFPDVDISLGTRPDQRIGSDENWDRAEAVLGSALEKLGRPYVVTPKDGNFYGPKID
                     FRVKDAIGRPWQLGTMQVDFAQPELFDLTYIGEDGKPHRPVVLHRAILGSVERFLGIL
                     IEHTAGNFPFWIAPVQAVVLPVSDRFAAAARETAGRLFAAGLRVETDDRNEKLGARIR
                     RAELFKVPAMLVVGEKEASAGAVAVRLRHGGDAGTMSVDDFVAAARRAIAARSRELTT
                     EVKDR"
BASE COUNT          496 a         1063 c         1023 g          644 t
ORIGIN      
        1 cgagctcgcg ccgccgcgcc tcctcgtgag cgagcgattc acccaggcgg gcgatcccct
       61 cctcgacctc ccgcttggcg gcgttcgcgc gcgcgccata ggcggcgcgg tccggcgggg
      121 gaatctcccg catccgcgcg aagagagcgg gaacgagccc cttctcgcgg ccgaggtagc
      181 gccccttgag cgcttcgaac tcggagcgcg aggcgactcc gcgcgccgcc tcctcggcga
      241 aggaggcgag cacgttcgaa atggggtcgt ccgccgggcc gatcggaccg ggttctccgg
      301 tcacgcgccg ccgagcgctt gcttcgccac ccccacgagg tgggtgaacg cgggcggatc
      361 ggtcaccgca aggtcggcga gcatcttgcg gttgatgtcg ttgccggcct tcttcaggcc
      421 gtccatcaac ttgctgtacg agatctcgtt ctcgcgcgcg gcggcattga tgcggacgat
      481 ccacagcccg cggaaatcgc gtttgcgcgc cttacggtcc cggtacgcgt agagcagcga
      541 cttctcgacc gcgagcttcg ccattcggta cgacttggat ttggttccgt agtagccctt
      601 cgccagcgcg agcgttttcg cgcgccggtt ctttctcttc ggtcctctct tgacgcgagg
      661 cattttcgaa tctccttccc tttgtcattc cgaggccgaa gagccgagga atctcgttca
      721 gcgagatccc tcgcttcgct cccaggacga cagacggggc gattacgcgt agggcagcat
      781 ctccttgacg cgcttgtggt ccgacgcgga aacggacgtg gactgcgcga gaccgcgctt
      841 ccgccccggc gctttcttcg tcaggatgtg gctcttgtag gccttcgctc tcttgaatct
      901 gcccgatgcg gtgcgtcgga aacgtttcgc agcgccacgg tgggtcttca atttcggcat
      961 gttcgtgtac tccctcgtta cgctccgcgg gccgccttct cgtgcacttt cacgggcgcg
     1021 agaatctgat agaggcggtt accgagcatc tccgaccgcg actcggcgac ggctttcccg
     1081 gcgagatccc cgagcagccg gtcgaggacc ttctgtccgt tctcgcggcg cgccatctcg
     1141 cgaccgcgga actggacggt cgccttcact ttgtcgccgt gcgccaggaa cttcaggatg
     1201 ttcttgatct tgaagtcgta gtcgtggtcg tcgatgttcg gtcggaactt cacttctttg
     1261 acctgagtcg ttttctgctt cttcttcgag tcctgctgct tcttcttctg ctggtagatg
     1321 aactttccga agttcatgat ccggcagacc ggcgggttgg cggtcggcgc gatctcgact
     1381 aagtcgagcg ctgcgtcctt ggccttctgg agcgcgtcgg ccatcgacac gatgccgagc
     1441 ggctctccct ccgctccgat caggcggact tccttccacc ggatctgttc gttgattcga
     1501 atgctcttct tatcgatctt tcacctccgt cgtgagctcc cggctcctcg ccgcgatggc
     1561 gcgtcgcgcg gccgccacga aatcgtccac actcatggtt cccgcgtcgc cgccgtggcg
     1621 cagccgcacc gcgaccgctc cggccgacgc ctccttctca ccgacgacga gcatcgcggg
     1681 cactttgaag agctccgccc gccggatccg cgcgccgagc ttctcgttgc ggtcgtccgt
     1741 ctcgacccgc agtcccgccg caaaaagtcg accggcggtc tcccgggccg ccgccgcgaa
     1801 cctgtcggag accggcagga caaccgcctg caccggcgcg atccagaacg ggaaattgcc
     1861 ggcggtgtgc tcgatcagga tcccgaggaa ccgttcgacc gatccgagga tggcgcggtg
     1921 gaggacgacc ggccgatgag gcttgccgtc ttcgccgatg tacgtcagat cgaagagctc
     1981 gggctgcgcg aagtcgacct gcatcgtccc gagctgccac ggccggccga tcgcgtcctt
     2041 cacgcgaaag tcgatcttgg ggccgtagaa gttcccgtcc ttcggggtca cgacgtaggg
     2101 gcgcccgagc ttctccagag cggatcccaa caccgcctcc gcgcggtccc agttctcgtc
     2161 cgagccgatg cgctggtccg gccgcgttcc gagcgagatg tccacgtcgg ggaagtcgaa
     2221 cgtccggtac acgtcgtcca ggaactccac gaacctggcg atctccggtt cgatctcgcc
     2281 gatcgggcaa tagatgtgag agtcgtcctg ggaaaaagtc cggacgcggg tcaggcccgc
     2341 cgtgacaccc gagcgctcgt accgatggag ccgcccgaag tccgcgtacc gaaccggcag
     2401 ctcccggtag gaccagagcc gttccttgaa gagcaggtgg tgtcccgggc agttcatcgg
     2461 cttgacgccg aactcgcgct cctcgatctc cgtgaagtac atgttgtctc tgtagttgtc
     2521 gtagtgcccg gagatcttga acagctcggc gtcgtagatc tgcggagtga tcacttcgtc
     2581 gtaaccgcga cgccgatact cctcgcgaag gtaggcgaga aggccgttgt agagaaccgc
     2641 ccccttcgga agaaagaacg gcgaagcgga agcgagcggg tgaaacgtga agagctcgag
     2701 ctcccgtccg agcttgcgat ggtcgcgcgc cttcgcgtcc tcgagccgct tcaggtactc
     2761 gtccagctcc ttctgcgtca ggaacgacgc cccgtagatc cgctggagca tcgggttctt
     2821 ctcgtcgccc ttccagtacg cgccggccgt gtgggtcagc ttgaagacgc cgagccgccc
     2881 ggtcgaggcg acgtgcgggc cgcggcagag gtcgatgaac tcaccctgtc ggtaacagga
     2941 gacgaactcg tcggggagct cccgcgcaat ctggaccttg aggggatcgt tctgtctttc
     3001 gaagatcgaa atggcctcgg aaacgggcat ttcggagcgg gtgatcggga ggtcttcccc
     3061 gatgatgcgg cgcatctccg cggagatctc gtcgaggtcc tcgggcgtga acggccgctg
     3121 cggcaggaag tcgtagtaat agccgttctc gatcggcgga ccgatgccga tctgtacgtc
     3181 cgggaagagc ctcttgaccg cctgcgccgt caggtgcgcg gtcgag
//
LOCUS       DASWAV010000613         2522 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U2665
            k141_517211, whole genome shotgun sequence.
ACCESSION   DASWAV010000613 DASWAV010000000
VERSION     DASWAV010000613.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960316
            Sequence Read Archive: SRR10260007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2522)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2522)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:47:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,929
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2522
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_517211"
                     /isolate="SMAG_U2665"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.36444 N 132.31889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..272
                     /gene="nadA"
                     /locus_tag="VF958_12710"
     CDS             <1..272
                     /gene="nadA"
                     /locus_tag="VF958_12710"
                     /EC_number="2.5.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014398235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008987 - quinolinate synthetase A
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HEX9150016.1"
                     /translation="QFVTTAPAKKFLIATEEGIIHQMQKIAPDREYIPIPPDNGCRCN
                     ECPFMKLNTLEKLYLCLRDERPEIVMPEELRKAAEKPLLRMLELS"
     gene            290..1144
                     /gene="nadC"
                     /locus_tag="VF958_12715"
     CDS             290..1144
                     /gene="nadC"
                     /locus_tag="VF958_12715"
                     /EC_number="2.4.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003045538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004514 - nicotinate-nucleotide
                     diphosphorylase (carboxylating) activity [Evidence IEA];
                     GO_process: GO:0019363 - pyridine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HEX9150017.1"
                     /translation="MDGLSAAELDETLRRFLAEDVGSGDVTTDSIVPEDARIAAEIVA
                     KSECVVSGLPISRRVFELLDDSLSWEERVRAGGKVIPATTLARLSGRARAILTGERVA
                     LNLLQRMCGIATATRRYVEAVSGTGCRILDTRKTAPGLRAFDRVAVRDGGGTNHRAGL
                     YDMVLVKDNHRAIAGGVRSALEAARARVPAGMTIEVEVESEEDLREAVGGGADVLLID
                     NQTPETVRRWVDIARRDARSPQIEASGNMTLERVRDYALAGVDSISVGALTHSVAAAD
                     VSLEVEIG"
     gene            1141..>2522
                     /locus_tag="VF958_12720"
     CDS             1141..>2522
                     /locus_tag="VF958_12720"
                     /inference="COORDINATES: protein motif:HMM:NF013086.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEX9150018.1"
                     /translation="MTKDGTEVLVLGTGIAGCAAAIAAAREGADVTLVTRASELFETN
                     TFYAQGGIVARPPGDSPELLAEDIHRAGDGLCDPEAVDLIAREGPDLVRELLIDELRV
                     PFDREGDDFRWTLEGAHSVPRILHVKDETGVPIERALLDACRNEKNVRILASTTAVDL
                     LTLAHDSVNPTDRYDPPTVVGAYLLERATGEVRPLLAGETILATGGLGQVYLHTTNPQ
                     GARGDGVAMARRAGARLLNLEFIQFHPTALVHPKGRLLLTEALRGEGAKITDASGQSF
                     LKDVHPDAELAPRDVVARAIHQRMLERDEPYAYLDISHKPADWIRERFPGLTKRCLEY
                     GFDLTAGPIPVVPAAHYSCGGVSVDLDGATTLAHLRAAGEVACSGVHGANRLASTSLL
                     EGLLWGWRSGKAAVAAIRSEPLRRRVEVRPWEPEFEPVDPALIAQDWLVVRHTMWNYV
                     GLLRSEKRLDR"
BASE COUNT          403 a          865 c          874 g          380 t
ORIGIN      
        1 tgcagttcgt gacgacggcg cccgcgaaga agttcctgat agcgaccgag gaggggatca
       61 tccaccagat gcagaagatc gccccggacc gcgaatacat cccgatccct cccgacaatg
      121 gctgccgctg caacgagtgt ccgttcatga agctgaacac cctcgagaag ctgtacctgt
      181 gcctgcgcga cgagcggccc gagatcgtga tgcccgagga gctccgcaaa gcggccgaga
      241 agccgctttt gagaatgttg gagctgtcct agcgatatcc tcccccggaa tggacgggct
      301 ttccgccgcg gagctggacg aaacgctccg tcgtttcctg gcggaggacg tcggaagcgg
      361 ggacgtgacg accgattcga tcgttcccga ggatgcccgg atcgccgccg agatcgtcgc
      421 gaagtccgag tgcgtcgtct ccggcctccc gatctcccgg cgggtcttcg agctcctcga
      481 tgattctctt tcctgggagg agcgcgtgcg ggccgggggg aaggtcatcc ccgcgacgac
      541 cctcgcgcgt ctctccggca gggcacgcgc gatcctcacc ggagagaggg tcgcactgaa
      601 cctcctgcag cgcatgtgcg ggatcgcgac cgcgacgcgt cgttacgtcg aggcggtatc
      661 cggcaccggc tgccggatcc tggacacgcg caagacggcg ccgggtctgc gcgctttcga
      721 tcgggtcgcc gtgcgcgacg gcggcggaac gaaccatcgg gccggcctgt acgacatggt
      781 tctcgtcaag gacaaccacc gcgcgatcgc gggtggcgtg cgatcggccc tggaagcggc
      841 gagagcccgc gttcccgcag ggatgacgat cgaagtcgag gtcgagtcgg aggaggactt
      901 gcgcgaggcg gtcggcggcg gcgcggacgt gctcctgatc gacaaccaga ccccggaaac
      961 cgtgcgccgc tgggtggaca tcgcacgtcg ggacgcccgc tcgccgcaga tcgaggcgtc
     1021 ggggaacatg accctggaga gggtgcgcga ctacgcgctc gccggagtcg actcgatctc
     1081 cgtcggcgcg ctcacgcatt cggttgccgc cgcggacgtt tcgctggaag tggagatcgg
     1141 atgacgaagg acgggaccga ggtcctcgtg ctcgggacgg ggatcgcggg ctgcgcggcg
     1201 gcgatcgcgg ctgcccgcga aggcgccgac gtcacgctcg tgacgcgcgc gagcgagctc
     1261 ttcgagacga acactttcta tgcccaggga ggcatcgtcg cgcgcccccc gggcgattcg
     1321 ccggagctcc tcgccgaaga catccaccgc gcgggcgacg gactctgcga cccggaggcc
     1381 gtggacctga tcgcccgcga aggaccagat ctggttcgcg agctcctgat cgacgagctg
     1441 cgggtcccgt tcgaccgcga gggggacgac ttccgctgga cgctcgaggg cgcgcattcc
     1501 gtcccgcgaa tcctgcacgt gaaggacgag accggtgtgc cgatcgagag ggcgctcctc
     1561 gacgcctgcc ggaacgagaa gaacgtgcgg atcctcgcca gcacgacggc cgtcgacctg
     1621 ctcacgctgg cgcacgattc cgtgaacccg acggatcgct acgacccgcc caccgtggtc
     1681 ggcgcgtacc tgctcgagcg cgcgaccggc gaggtccggc cgctcctcgc cggcgagacg
     1741 atcctcgcga caggaggcct cgggcaggtc tacctccaca ccacgaatcc gcagggcgca
     1801 cgcggcgacg gggtcgcgat ggcgcggcgc gcgggcgcgc gcctcctgaa cctcgagttc
     1861 atccagttcc acccgaccgc tctcgtgcat ccgaagggcc gcctgctcct gaccgaagct
     1921 ttgcgcggcg aaggcgcgaa gatcaccgac gcctccgggc agtcgtttct gaaggacgtg
     1981 catcccgacg ccgagctcgc gccgcgcgac gtcgtggcgc gcgcgatcca tcagcgcatg
     2041 ctcgagcgcg acgagccgta cgcgtacctc gacatctccc acaagcccgc ggactggatt
     2101 cgcgagcggt tcccgggtct cacgaaacgg tgcctcgaat acggcttcga cctgaccgcg
     2161 ggtccgatcc cggtcgtccc cgccgctcac tactcctgcg gcggcgtctc cgtcgatctg
     2221 gacggcgcga ctacgctggc gcacctgcgc gcggccgggg aagttgcctg cagcggcgtc
     2281 cacggcgcga accgcctcgc gtcgacatcg ctcctcgaag ggctgctctg gggctggcgg
     2341 tcgggaaaag cggccgttgc agcgatccgg tccgagccgc tccggcggcg cgtcgaggtc
     2401 cgcccttggg agccggagtt cgaacccgtc gacccggcgc tgatcgccca ggactggctc
     2461 gtcgtgcgtc acaccatgtg gaactacgtc gggttgcttc gttcggaaaa acggctcgac
     2521 cg
//
LOCUS       DASWAV010000614         4028 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U2665
            k141_114851, whole genome shotgun sequence.
ACCESSION   DASWAV010000614 DASWAV010000000
VERSION     DASWAV010000614.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960316
            Sequence Read Archive: SRR10260007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 4028)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4028)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:47:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,929
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4028
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_114851"
                     /isolate="SMAG_U2665"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.36444 N 132.31889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(107..1339)
                     /locus_tag="VF958_12725"
     CDS             complement(107..1339)
                     /locus_tag="VF958_12725"
                     /inference="COORDINATES: protein motif:HMM:NF018004.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1015 family protein"
                     /protein_id="HEX9150019.1"
                     /translation="MELRPFRSIRYSRATIEERGLSALIAPPRRVESPAPGNIARLTF
                     AEDADAAAKTHQNWLAGGILEKERRPALWLYRQTFFVDGRPFVRDALVGLVRIGDGEK
                     GSVLDPQDPPPPELVLQRLALLRAMRADFTPSFIVTHAPLSGRLATTRAPELSAVDPD
                     GVRHDAMRIADYAAHVELQGLVKNVEAILADGGDRLIAAQEYSTDPAAAKFGGAKYKL
                     CAIVEMESPGLFMRPVHRLLRGIPGWNPERLISEAKDFFETRELATPADALPALGQVS
                     RLRAAFALVAPPAKPTLFVLRDRPAALPWPEGRSESWRGVDTAVLEVAFFSRLLEIDR
                     EQSARDGQVAFTSDSDAAVAATGSGDAQAAILMRPLTLSEIETVVHTGDRLPPRSVSF
                     YPEMFAGLFGVSLEDPVY"
     gene            1378..2304
                     /gene="xerC"
                     /locus_tag="VF958_12730"
     CDS             1378..2304
                     /gene="xerC"
                     /locus_tag="VF958_12730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015722151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009009 - site-specific recombinase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HEX9150020.1"
                     /translation="MITLARAIDEFETYVADARRYSPQTVRAYRSDLDRFAEFWEREF
                     GESGASKTPLSRVDTLAVRSYLASLHRARLASRSLARHLSTLRSFFRWACREGHLEKN
                     PARAIPAPRLPKTLPRAMTLPDTERLLEADEDAPFPERDRALFELLYATGLRVSEAAG
                     LDLEDVDFASRLLRVIGKGNKERIAPFGETAGDSLAAYLPERARRRKGNPAAEREEGE
                     PLFVNARGGRLTTRSMARLLKHRLRAAGLPAEISPHALRHSFATHLLEAGADLRAIQE
                     LLGHASLSTTQKYTHLDAARLREVYRRAHPKA"
     gene            2333..2878
                     /gene="hslV"
                     /locus_tag="VF958_12735"
     CDS             2333..2878
                     /gene="hslV"
                     /locus_tag="VF958_12735"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013405855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004298 - threonine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0051603 - proteolysis involved in protein
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease subunit HslV"
                     /protein_id="HEX9150021.1"
                     /translation="MNPGEKWHSTTVVCVRHGDRVAMASDGQVTLGATIMKKTAAKIR
                     RLAGGSVLAGFAGSSADAFALFSRFEMKLDEYRGNLERAAIELAKDWRTDKMLRQLEA
                     LLIVADKSSSLLISGTGDLISPEEGILAVGSGGPYALAAARALVKHRPDMPAADVARE
                     SLAIAATIDIYTNDSITLEEI"
     gene            2892..>4028
                     /gene="hslU"
                     /locus_tag="VF958_12740"
     CDS             2892..>4028
                     /gene="hslU"
                     /locus_tag="VF958_12740"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020721668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATPase subunit HslU"
                     /protein_id="HEX9150022.1"
                     /translation="MPGETTASAETPKPVQARTPREIVGELDKFVIGQGRAKRSVAIA
                     LRNRWRRQQLDPEFAREILPKNILMIGPTGVGKTEIARRLARLADAPFLKVEASKFTE
                     VGYVGRDVESIIRDLTEVAVQMVREEKTVDIRERARARVEQRLMELLQPSFTDADIEE
                     ARGRLRQMLREGRLEDRLVEVEVADKTFPSFEIVTDKGVEEMDINVKEMLPGLFGGKK
                     KRRKTTVKDAREILQAEEEGRLIDAGQVAREAVARAETSGIVFLDEIDKVAGRESGRS
                     GPDVSREGVQRDLLPIVEGTVVNTKYGFVKTDHVLFIAAGAFHVSKPSDLIPELQGRF
                     PIRVELDSLTEEDFVRILTEPENALLKQYRALLSAEGVDLAFTDD"
BASE COUNT          673 a         1365 c         1408 g          582 t
ORIGIN      
        1 gagagcctgg tgaagaacga cgtgcgcggc ttcgctacga agaagaaggc gaagtgaccc
       61 gccgcaggcg ggtcatcccg agcgaagcga gggatctttc gcttcctcag tagaccggat
      121 cttcgagcga tacgccgaag agacccgcga acatctccgg gtagaagctc acggatcgcg
      181 gcggcaggcg gtcgccggtg tgaacgaccg tctcgatctc cgagagcgtg agcggccgca
      241 tgaggatcgc cgcctgcgca tcaccgctcc cggtggccgc cacggccgcg tcggagtcgc
      301 tcgtgaacgc gacctggccg tcgcgagcgc tctgctcccg gtcgatctcg aggagccgcg
      361 agaaaaacgc gacctcgaga accgcggtgt cgacgccgcg ccatgactcg gaccgcccct
      421 ccggccacgg cagcgccgca gggcggtcgc gcaggacgaa cagggtcggc ttcgcgggcg
      481 gtgcgaccag ggcaaacgcc gctcgcagcc gcgagacctg gccgagcgcc ggcagagcgt
      541 ccgccggcgt cgcgagctcg cgtgtctcga agaaatcctt tgcctcggag atcagccgct
      601 cggggttcca gccggggatt ccccgaagaa gccggtgaac cggccgcatg aaaagacccg
      661 gcgactccat ctccacgatc gcgcacagct tgtacttcgc accgccgaat tttgccgccg
      721 ccgggtccgt cgagtattcc tgggccgcga tgagccggtc tccgccgtcg gcgaggatcg
      781 cctcgacgtt cttcacgagt ccctgcagct ccacgtgcgc cgcgtagtcg gcgatcctca
      841 tcgcgtcgtg ccggaccccg tcggggtcga cagcggagag ctccggggcg cgcgtggtcg
      901 cgagccgacc cgagagcggc gcgtgggtca cgatgaacga cggggtgaag tcggctcgca
      961 tcgcgcgcag gagtgcgagc cgctgcagga cgagctcggg cggaggcggg tcctgcgggt
     1021 cgaggaccga acctttctcg ccgtcaccga tgcggaccag gccgacgagc gcgtcgcgca
     1081 cgaacggccg gccgtcgacg aagaacgtct ggcgataaag ccagagcgcg gggcggcgct
     1141 ctttctcgag gatgccgccc gcgagccagt tctggtgcgt tttcgccgcc gcgtccgcat
     1201 cctcggcaaa cgtgagccgc gcgatgttgc ccggcgcggg ggactcgacc cggcgcggcg
     1261 gcgcgatgag cgccgacagc ccgcgctcct cgattgtcgc acgcgagtat cgaatcgaac
     1321 gaaacggtcg cagctccacg gcggggcgga ttttaagcgc gagtacactg ttcgcgagtg
     1381 atcacgctcg cgcgcgcgat cgacgagttc gaaacttacg tcgccgacgc ccgccggtac
     1441 tcgcctcaaa cggtgcgcgc gtaccgcagc gatctcgacc ggttcgccga gttctgggag
     1501 cgcgagttcg gcgaaagcgg cgcctcgaag acgcctctct cccgggtgga taccctcgcc
     1561 gtccgctcgt atctcgcgtc gctccaccgc gcgcgcctcg cgagccgatc gctcgcgcgt
     1621 cacctctcca cgctgcgctc gttcttccgc tgggcgtgcc gcgaagggca cctggagaag
     1681 aatcccgccc gcgcgattcc cgctccgcgg ctgccgaaga cgctcccgcg cgcgatgacg
     1741 cttcccgaca ccgagcgcct cctcgaggcg gacgaggacg cgccattccc ggagcgcgac
     1801 cgcgcgctct tcgagctgct ttatgcgacg gggctgcgtg tgtcggaggc ggccgggctc
     1861 gacctcgagg acgtcgactt cgcgtcgcgc ctgttgcgag tcatcggaaa ggggaacaag
     1921 gagcggatcg cgccgttcgg cgagacggcc ggggactcgc tcgccgcgta cctgcccgag
     1981 cgcgcacggc ggcgaaaggg aaatcccgcg gcggaacggg aagagggcga gcctctcttc
     2041 gtcaacgcgc gcggcgggcg gttgacgacg cgctcgatgg cgcggctgtt gaagcaccgg
     2101 cttcgcgccg cggggctgcc cgcagagatc tcgccgcacg cgctgcgtca ctcgttcgcg
     2161 acccatctgc tcgaggccgg ggccgacctg cgcgcgatcc aggagctgct cggccacgcg
     2221 tcgctctcga cgacgcagaa gtacacgcac ctggacgccg cgcgcctgcg cgaggtgtac
     2281 cgccgggccc accccaaggc gtgaggcaaa acgcggtatc ctcgttttcc gaatgaatcc
     2341 gggcgaaaag tggcattcca ccacggtcgt ctgcgtgcgg cacggcgacc gcgtggccat
     2401 ggcctccgac ggccaggtca cgctcggcgc cacgatcatg aagaagaccg ccgccaagat
     2461 ccgacggctc gcggggggca gcgtgctggc cggtttcgcc ggctcgtcgg ctgacgcgtt
     2521 cgcgctgttc tcgcggttcg agatgaagct cgacgagtac cgcggcaacc tggagcgcgc
     2581 cgcgatcgag ctcgcgaagg actggcggac cgacaagatg ctccgacagc tcgaggcgct
     2641 cctgatcgta gccgacaagt cgagctcgct cctcatctcc ggcacgggcg acctgatctc
     2701 acccgaggag ggcatcctcg cggtcggctc cggagggccg tatgccctgg cggcggcgcg
     2761 cgcgctcgtc aagcaccgcc cggacatgcc ggccgccgac gtcgcgcgcg agtccctcgc
     2821 gatcgccgcg acgatcgaca tctacacgaa cgacagcatc accctggagg aaatctgagg
     2881 tggtctactt gatgccgggc gagacgacgg caagcgccga gacgccgaaa cccgtacagg
     2941 cgcgcacgcc gcgcgagatc gtgggagaac tcgacaagtt cgtgatcgga caggggcgcg
     3001 ccaagcgctc ggtcgcgatc gcgctgcgca atcggtggcg ccgccagcag ctggatcccg
     3061 agtttgcgcg cgagatcctc ccgaagaaca tcctgatgat cggtccgaca ggcgtcggaa
     3121 agaccgagat cgcgcggcgg ctcgcccgcc tcgcggacgc gccgttcctg aaggtcgagg
     3181 cgtccaagtt caccgaggtc ggctacgtcg gccgcgacgt cgagtcgatc atccgcgacc
     3241 tgacggaggt cgccgtgcag atggtgcgtg aagagaagac cgtcgatatc cgtgagcgcg
     3301 cgcgggcgcg cgtcgagcag cgattgatgg agctcctcca gccttcgttc accgacgcgg
     3361 acatcgagga ggcgcgcggg cggctccggc agatgctgcg cgaggggagg ctcgaggacc
     3421 ggctcgtcga ggtcgaggtc gccgacaaga cgtttccctc tttcgagatc gtcaccgaca
     3481 agggcgtcga ggagatggac atcaacgtca aggagatgct ccccggcctc ttcggcggca
     3541 agaagaagcg gcgcaagacg acggtcaagg acgcgcgcga gatccttcag gccgaggaag
     3601 aggggagatt gatcgacgcc ggccaggtcg cgcgcgaagc ggtcgcgcga gccgagacct
     3661 ccggcatcgt attcctggac gagatcgaca aggtcgccgg ccgcgagtca ggaaggagcg
     3721 gtcccgacgt gtcgcgcgag ggcgtccagc gcgacctcct cccgatcgtc gagggcacgg
     3781 tcgtcaacac gaagtacggg ttcgtgaaga cggatcacgt gctcttcatc gcggcgggcg
     3841 cgtttcacgt ctcgaagccg tcggatctga tccccgagct ccaggggcgg ttcccgatcc
     3901 gcgtggagct cgactcgctg accgaggagg atttcgtccg catcctgacc gagccggaga
     3961 acgcactcct gaagcagtac cgggcgctcc tctcggcgga gggcgtggat ctcgcgttca
     4021 ccgacgac
//
LOCUS       DASWAV010000615         5330 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U2665
            k141_517552, whole genome shotgun sequence.
ACCESSION   DASWAV010000615 DASWAV010000000
VERSION     DASWAV010000615.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960316
            Sequence Read Archive: SRR10260007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 5330)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5330)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:47:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,929
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5330
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_517552"
                     /isolate="SMAG_U2665"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.36444 N 132.31889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..317
                     /locus_tag="VF958_12745"
     CDS             <1..317
                     /locus_tag="VF958_12745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="BRCT domain-containing protein"
                     /protein_id="HEX9150023.1"
                     /translation="KGVHLAFTGILARRRDEAVRSAKRAGAIVHGSPSARTTVVVRGR
                     PNPLQAAGRDAGVKLMEIKRLREKGHRITLLDERRFWRLAGRPLPPGTGRGGRTSLSG
                     RA"
     gene            complement(301..1122)
                     /gene="arsM"
                     /locus_tag="VF958_12750"
     CDS             complement(301..1122)
                     /gene="arsM"
                     /locus_tag="VF958_12750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013218845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenite methyltransferase"
                     /protein_id="HEX9150024.1"
                     /translation="MTTTTNDIREAVRERYGHIAEHGSSVGCCGPTESGRGCGCGTSM
                     TVTTERMDALGYTDEQRAAIPSGADLGLGCGNPLAHAGVAKGETILDLGSGAGIDAFL
                     AAQETGPSGRVIGVDMTSAMLSRARENAAKHGFGNVEFRLGEIESLPVADGTVDLIIS
                     NCVVNLSPDKPRVFREALRVLRPGGRMVVSDIVLVAELPASVRSSVEAYVGCVAGASM
                     KNEYLKALREAGFELVEIVEEKRYGTAGLEMEDEIASRTAEAVRSVKVRAFKPAR"
     gene            complement(1119..1493)
                     /locus_tag="VF958_12755"
     CDS             complement(1119..1493)
                     /locus_tag="VF958_12755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020732921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HEX9150025.1"
                     /translation="MSESRPPKPAGRALLPVVKTPRPRGVRADDEELARLAKAIGHPA
                     RAAIVRLLLAGGECVCGGIVDRLPLAQATVSQHLKVLKEAGWITGEVDGPRVCYCAKP
                     ETLRRFVALGRDLDPKDERSGS"
     gene            complement(1646..4078)
                     /locus_tag="VF958_12760"
     CDS             complement(1646..4078)
                     /locus_tag="VF958_12760"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HEX9150026.1"
                     /translation="MIGQTISHYRVLRVLGEGGMGVVYEAEDIRLGRHVALKFVVHKK
                     LDRQETVERFEREARAASVLNHPNICTVHDVGEDAGHHFIAMELLEGENLDALLTRGP
                     LPIRQVLDLGVQVAEGLAAAHARGVIHRDIKPANVFVTSAGFVKIMDFGLAKILPSPA
                     AEALTDSESDLTVTSGLTTPGVTMGTVSFMSPEQVRGEEVDARTDLFSFGALLYDMAV
                     GEPPFRGATPAVKLSAILERNPAPPLEKNPGLPAKLQDIILKALEKNREERYQSAREM
                     AVDLRRLLREVDSGAVTSSSGAVSIPRITAPVSPVGTGRRKYWVAAAVVAGAVLAGAL
                     FLWKPGGAPPRPTAPPARAEIKSLAVLPLDNLSQDPAQEYFADGMTDELIARLSKISA
                     LRVISRTSAMRYKGSKKSLPEIAKDLQVDAVVEGSVLRSGNRVRINARLIDAAERQMW
                     SESYDRELRDILSIQSDVASAIVREIRVKVAPEEITQIASKGQVDPEAYQLYLQGRVA
                     FLRFTPESLAEAEEYFNAAIAKDPRYALAYTGLADSYIQLAGRARPPRETMPGAREAS
                     ERALSIDPNLAEAHASLGQVKLFYEFDWDGARTEYRRALEKNPGSPLVHQANALYLAA
                     RGRTAESLAEGDRVLDLDPVSSSSGCLRTRLLYYARRYDESIAEYERTRTVDPNVAGF
                     CMFAIFAYQQRGRFDEAIAASKRISEASPNEVIPRAALARSYGVMGNRVEAEKVIRAM
                     EDLSKRRFISEHDFAVAHSGWDKKGTLEWLEKGYRGRVGLLVFARVDSVWDDLRAEPR
                     FQELVRKVGIPQ"
     gene            complement(4242..4592)
                     /locus_tag="VF958_12765"
     CDS             complement(4242..4592)
                     /locus_tag="VF958_12765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009050012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HEX9150027.1"
                     /translation="MSPRSAASAELGRASPVFAALGDETRLAVVARLCARGPQSITRL
                     TTGSAVTRQAITKHLNVLAGAGLVRDVRRGRERIWELDTKRLDDASRWLDQISKRWDE
                     ALGRLKRFVEAPSL"
     gene            complement(4579..5028)
                     /locus_tag="VF958_12770"
     CDS             complement(4579..5028)
                     /locus_tag="VF958_12770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019692848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HEX9150028.1"
                     /translation="MSTDRIERTVFLRAPRSRVWRAIATAEEFGTWFGVKLEGEFSVG
                     ASVSGRITIPGYQHVTMEITIERVDSERLFSYRWHPYAIEPDVDYSGEPTTLVEFRLE
                     EVTGGTKLTVTESGFDRIPAARRAEAFRMNEQGWTGQIRNIEHHVAP"
     gene            5148..>5330
                     /locus_tag="VF958_12775"
     CDS             5148..>5330
                     /locus_tag="VF958_12775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9150029.1"
                     /translation="MAALATVVTAPLFAQLPPLPPLPGLEIRIGRQAPPRPRRERRTA
                     RPNRDSVWVQGFWHWEG"
BASE COUNT          846 a         1850 c         1727 g          907 t
ORIGIN      
        1 tgaagggcgt acatctcgcg ttcaccggaa tcctcgcccg gcgccgcgac gaagccgtcc
       61 gctccgcgaa gcgggcggga gcgatcgtcc acggcagccc ctcggcgcgg acgaccgtcg
      121 tcgtgcgcgg gcggccgaat ccgctccagg cggcgggccg cgacgcggga gtcaagctga
      181 tggagatcaa gcgccttcgc gagaaaggac accggatcac gctgctcgac gagaggcgct
      241 tctggcgact ggcgggacgc cccctccccc cgggaacggg gagagggggt cgtacctctc
      301 tcagcgggcg ggcttgaaag cccggacctt gaccgatcga acggcctccg ccgtccgtga
      361 cgcgatctcg tcttccatct cgagcccggc ggtgccgtag cgtttctcct cgacgatctc
      421 gacgagctcg aagccggcct cccggagagc cttcaggtat tcgttcttca tcgacgcacc
      481 ggccacgcag cccacgtatg cctccaccga ggagcgaacg gacgcgggca gctccgccac
      541 gagcacgatg tcggagacga ccatccgccc gccgggccgg agcacccgca gcgcttcacg
      601 gaagacgcgc ggcttgtcgg gagacaggtt gacgacgcag ttggagatga tgaggtccac
      661 cgtgccgtcg gcgaccggaa ggctttcgat ctcgcccagc cggaactcga cgttcccgaa
      721 tccgtgtttc gccgcgttct cccgggcccg cgaaagcatc gcggaagtca tgtccactcc
      781 gatcacccgg cccgacggtc ccgtctcctg cgccgcgagg aacgcgtcga ttccagcgcc
      841 cgaaccgagg tcgagaatcg tctcgccttt ggcgacaccc gcgtgtgcca gcggattccc
      901 gcaaccgagg cccaggtccg ccccgctcgg aatcgcggcc cgctgctcgt cggtgtaacc
      961 gagagcatcc atcctctcgg tcgtcacggt catgctggtg ccgcagccgc atccgcggcc
     1021 ggactcggtg ggcccgcagc agccgaccga ggagccgtgc tccgcgatgt gtccgtagcg
     1081 ctcgcgcacg gcctcccgga tgtcgttcgt cgtcgtcgtc atgatccact cctttcatcc
     1141 ttcggatcga gatcccgccc cagcgcgacg aagcgccgga gtgtctccgg tttcgcgcaa
     1201 taacagacgc gcgggccgtc cacctcgccg gtgatccagc ccgcctcctt caagaccttc
     1261 aaatgctgcg acacggtcgc ctgggcgaga ggcagtcggt cgacgatgcc cccgcagacg
     1321 cactccccac ccgcgaggag aaggcgaacg atcgccgcgc gcgcgggatg gccgatcgcc
     1381 ttcgccagcc gtgccagctc ttcgtcatcg gcgcgcacgc cccgcggccg cggcgtcttc
     1441 accacgggga ggagggcccg cccggccggt ttcggaggtc gcgattcact catcgttgat
     1501 cgtagatata cgataaaacg acggatgtca agaggccgat gacgatccga tcccccgagc
     1561 ggccggcccg ggtctatcgg gcgggcggga aacctttcag catcttcgag atagcccggg
     1621 agagcgcttc ctctttctcg gcggcttact gcggtatccc gaccttccgg acgagctcct
     1681 ggaaccgcgg ctcggcgcgc aggtcgtccc acacggagtc cacccgagcg aacacgagca
     1741 acccgacgcg cccccggtag cccttctcca gccactcgag ggttcccttc ttgtcccacc
     1801 cggaatgcgc cacggcgaag tcgtgctcgg agatgaacct ccgtttcgac aggtcctcca
     1861 tcgcccgaat caccttctct gcctcgaccc ggttccccat cactccatag ctgcgcgcga
     1921 gagcggcccg cggaatcacc tcgttcggtg aagcctcgct gatccgcttc gatgctgcga
     1981 tcgcctcgtc gaaccgaccc ctttgctggt acgcgaagat cgcgaacatg cagaaaccag
     2041 cgacgttcgg gtccacggtc cgcgtcctct cgtactctgc gatcgactcg tcgtaccgcc
     2101 gcgcgtagta gaggagccgc gttcggaggc aacccgagct cgagctcaca ggatcgagat
     2161 cgagcacgcg gtcgccttcc gccagcgact ccgccgtgcg cccccgggcg gcgaggtaga
     2221 gggcgttcgc ctgatgaacg agcggcgagc ccggattctt ttcgagcgcg cgccgatatt
     2281 ccgtccgggc gccgtcccag tcgaactcgt agaaaagctt gacctgcccg agcgatgcgt
     2341 gggcttccgc caggttcggg tcgatcgaca acgcccgctc gctcgcctct cgcgccccgg
     2401 gcatggtctc gcgaggggga cgggcgcggc ccgcgagctg gatatagctg tcggcgagcc
     2461 ccgtgtatgc gagggcatac cgtggatcct tcgcgatcgc ggcgttgaag tactcctcgg
     2521 cttcggcgag cgactccggg gtgaaccgca ggaacgcgac ccgcccctgc agatacagct
     2581 gatacgcctc gggatccacc tgacccttgc tcgcgatctg tgtgatctct tccggtgcca
     2641 ccttcacgcg gatttcccgc acgatggcgc tcgcgacgtc gctctggatc gaaaggatgt
     2701 cacgcagttc gcggtcgtag ctctccgacc acatctgccg ctcggcggcg tcgatcagcc
     2761 gcgcgttgat gcggacgcga ttgccggatc ggagcacgga cccttcgacg acggcgtcca
     2821 cctggagatc cttcgcgatc tcgggaagcg acttcttcga acccttgtag cgcatcgcgg
     2881 aggtacgcga gatcacgcga agcgccgaga tcttggacag ccgggcgatc agctcgtccg
     2941 tcatgccgtc ggcgaagtat tcctgggccg ggtcctgcga aaggttgtcg agcggcagaa
     3001 cggcgaggga tttgatctcc gcacgtgcgg gcggcgccgt cggcctgggc ggcgctccac
     3061 cgggcttcca gagaaagaga gcgccggcaa gaaccgctcc cgcgacgacc gcggccgcga
     3121 cccagtactt ccggcgtccc gtccccacgg gcgagacggg ggcagtgatt cggggaatcg
     3181 acacggcgcc ggacgagctc gtgactgcgc cgctgtcgac ttcgcgcagc agtcgccgca
     3241 ggtcgacggc catctcgcgc gccgattggt atcgctcttc ccggttcttc tcgagcgcct
     3301 tcaggatgat gtcctggagt ttcgccggca gcccgggatt cttctccagg ggcggggcgg
     3361 gattgcgctc gaggatcgcc gagagcttca cggccggcgt cgccccgcgg aagggtggct
     3421 cgccgactgc catgtcgtag agcagcgcgc cgaacgagaa gaggtccgtg cgcgcgtcca
     3481 cctcttctcc tcgaacctgc tccggcgaca tgaacgacac cgtccccatc gtgacgcccg
     3541 gcgtcgtgag gccgctcgtc accgtcaggt ccgactccga gtccgtcaga gcttcggccg
     3601 ccggcgaagg gagaatcttc gccaggccga agtccatgat cttcacgaac ccggccgacg
     3661 tcacgaacac gttcgccggc ttgatgtccc ggtggatcac gccgcgggcg tgcgcggcgg
     3721 ccaggccttc ggcgacctgg acgccgagat cgaggacctg ccggatcggc agaggaccgc
     3781 gcgtcaggag ggcgtcgagg ttttcgccct ccaggagctc catggcgatg aagtggtgac
     3841 cggcatcttc gccgacgtcg tggacggtgc agatgttcgg gtgattgagg accgatgccg
     3901 cacgcgcttc gcgctcgaat cgctcgacgg tctcctgccg gtcgagtttc ttgtggacga
     3961 cgaacttcag cgcgacgtga cgcccgagac gaatgtcttc tgcctcgtac acgacgccca
     4021 tgccgccctc gcccagcacg cggagaacgc ggtagtggga aatcgtctgg ccgatcaatt
     4081 tcgtatcccg aaacgcccga acatattccg ttccattgtc tcgccgggag agcatcaagg
     4141 tgaagtggac caacgtcgta cgcaacagcg cgggctcacc ccgcgcgcgg aactcttctc
     4201 tctctacctc ctcctctccc cgtgagcggt cggggagagg gtcagagtga gggggcctcc
     4261 acgaacctct tcagccgacc gagcgcctcg tcccagcgct tggagatctg gtcgagccag
     4321 cggctcgcgt cgtcgagtcg cttcgtgtcg agctcccaga tgcgttctcg cccgcggcgt
     4381 acgtcgcgga cgaggccggc gccggccagc acgttcaagt gcttggtgat cgcctggcgc
     4441 gtgacggcgg accccgtcgt gagtcgcgtg atcgattgcg ggccgcgggc gcagagtcgc
     4501 gcgacgaccg cgagccgggt ctcgtcgccg agcgctgcga agaccgggct cgcgcggccg
     4561 agttcggctg atgccgcgct acggggcgac atgatgctcg atgttcctga tctgcccggt
     4621 ccagccctgt tcgttcatcc ggaaggcctc ggcccggcgt gccgccggga ttcgatcgaa
     4681 tccggactcg gtcaccgtga gtttcgtgcc gccggtgact tcctcgaggc gaaactcgac
     4741 cagtgtcgtg ggctcccccg aatagtccac gtccggctcg atggcgtagg gatgccagcg
     4801 gtaggagaac aggcgctccg agtcgacacg ctcgatcgtg atctccatgg tgacgtgctg
     4861 gtagccgggg atggtgatcc gacccgagac gctcgcaccc actgagaact ccccctcgag
     4921 cttcacgccg aaccaggtgc cgaactcctc ggccgtggcg atcgcgcgcc agacccgcga
     4981 tctcggcgcg cgcagaaaaa cggtcctctc gatccggtcc gtgctcattg tgcaacctcc
     5041 aggttgcata ttacaccaac gggccggatc gaagatggca cggcacttgc ggctttctcg
     5101 cggggatggg aggtttcatg ttcaaacgca tagtgagatc ggtcttgatg gcggcgcttg
     5161 ctacggtcgt gacggcccct ctgttcgcgc aactgccgcc gctacctcca ttgcccggcc
     5221 tcgagatccg gatcggccgc caggctcccc ctcggccccg ccgggaacgc aggacggcgc
     5281 ggccgaatcg ggactccgtc tgggtccagg gcttctggca ctgggagggc
//
LOCUS       DASWAV010000616         4222 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U2665
            k141_674961, whole genome shotgun sequence.
ACCESSION   DASWAV010000616 DASWAV010000000
VERSION     DASWAV010000616.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960316
            Sequence Read Archive: SRR10260007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 4222)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4222)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:47:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,929
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4222
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_674961"
                     /isolate="SMAG_U2665"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.36444 N 132.31889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1909)
                     /locus_tag="VF958_12780"
     CDS             complement(<1..1909)
                     /locus_tag="VF958_12780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9150030.1"
                     /translation="MIRRLRVPLAGAVLATPFLLFLYGRLPLPIGPRARSAALTSLQR
                     AFHDAVGDLTRRASDFQSQPEVARSLEGGGIAVNRLALFNAAGHSLASSPPGAGLALT
                     DASGIVHAWWGDAPSLENLTFSPEGLAVRWSATRLTAVQRRQVGDGGFSGVVYASQSF
                     PVDAPDFARALGLQGASAAWRPASTGGSPLLTDSSGTVLVTARPAAGTDVARLRNFGF
                     AALLVIAVALAGRLRDPLRAGLALAVAYLAVEARFGPASPLSFLTVAGLALGLAVLPG
                     RLVSFAGRPHTSPTPIALLAGVLLFGISLFVATRLETPELGEGVVQSLVALPRVAGLT
                     ALVLSALSLTGSRQTSPARRRTWATAAGLFTVAAIAGTLASVSATLSYRVLVLGVAVA
                     AFALWSGAVGEARAGDGSGGASLLIGSALLVVLVAAPLRDQERAREALRIASAIRLPD
                     PTRVSAEAVFAAHRAIERLSRTDLAGELPAPLERCDLSDLAYRLWREGEERLRQPPLM
                     TYEVFDPSGASRSSFSLIPEEERGSAAALPNEVVIDRYRLAILRRSVPLSYSGAPWGR
                     VEIEVADWPSWDPLPPRIDVYRRLVLGARGGSDDGPQPPRPVLASYAPDGSPREEGPA
                     LPTDAVQKIRHG"
     gene            complement(1906..3573)
                     /gene="hutU"
                     /locus_tag="VF958_12785"
     CDS             complement(1906..3573)
                     /gene="hutU"
                     /locus_tag="VF958_12785"
                     /EC_number="4.2.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012188185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016153 - urocanate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006548 - histidine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urocanate hydratase"
                     /protein_id="HEX9150031.1"
                     /translation="MPAVVAGPRVVRAARGTDLRCRGWAQEAALRMLENNLDPEVAEK
                     PDELIVYGGTGKAARNWECFDAIVETLKRLSSNETLLVQSGKPVGVFETHEDAPRVLI
                     ANALLVPAWATADEFRRLESAGLTMFGQMTAGSWIYIGTQGILQGTFETFAAAAEKEF
                     SGDLSGRLVVTAGLGGMGGAQPLAATMNGATALIAEVDGHRIERRLSTRYLDVREDSL
                     DRAVDRALESRDRREAVSIGIQVNAVDLLERLVARGIVPDLVTDQTSAHDELDGYVPR
                     NLSLEEAIALRREDPVTYVRRSYETMAAHVRALLEMKRRGAVVFDYGNNLRGQAKKAG
                     VENAFDIGGFVPLYIRPLFCEGKGPFRWAALSGDAQDIAETDRALLDLFPENAALRRW
                     LTLAKDRVAFQGLPARICWLGYGERARAGVLFNDLVRRGRVRAPIVIGRDHLDAGSVA
                     SPNRETEGMRDGSDAIADWPILNALLNTACGATWVSVHHGGGVGIGYSLHAGMVVVAD
                     GTDGAAKRLQRVLTADPGTGVMRHADAGYPEAIAAARERGVDLPTLR"
     gene            complement(3584..>4222)
                     /locus_tag="VF958_12790"
     CDS             complement(3584..>4222)
                     /locus_tag="VF958_12790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017950803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid lyase"
                     /protein_id="HEX9150032.1"
                     /translation="DAFDHARDVLEREMNSATDNPLVFAARGDVVSGGNFHGAPVGLV
                     LDYAAIAAADLASISERRIEKLVNPALSDLPAFLVEEGGLNSGLMMAQVTAAALVSEC
                     KALAHPASVDSIPTSAAKEDHVSMSPIAARKLAAIVENLAMVLAIELMVAFQAMEFLR
                     PLASSGPIERVRTRFRTIVAPWSSDRELAPDLAAARAFLDGGAISKLVESLA"
BASE COUNT          700 a         1425 c         1544 g          553 t
ORIGIN      
        1 cgccgtgccg gatcttctgc accgcatcgg tcggaagcgc cggcccctcc tcgcgcggcg
       61 agccgtccgg ggcgtaagag gcgaggacgg gacgcggagg ctggggaccg tcgtccgatc
      121 ccccgcgagc gccaagaacg agtcgccgat agacgtcgat gcggggcggc agcgggtccc
      181 agctcggcca gtccgccacc tcgatctcga cgcgtcccca cggcgccccc gagtaggaga
      241 gcgggaccga tcggcgaagg attgcgagtc ggtagcggtc gatgacgact tcgttcggga
      301 gagccgccgc ggagcctctc tcttcctcgg ggatgagcga aaagctgctc cgcgacgctc
      361 ccgacgggtc gaagacctcg tacgtcatga gcggcggctg gcgcagacgc tcctcccctt
      421 cgcgccagag ccgataagcg aggtcggaga ggtcgcaccg ctcgagcggc gcgggcagct
      481 cccccgcgag gtcggttcga gaaagacgtt cgatggcgcg atgcgcggcg aacaccgctt
      541 cggcggaaac gcgggtcgga tcgggcaggc gaatcgcgga cgcgatgcgc aacgcctctc
      601 gcgcgcgctc ctggtcgcgg agcggtgcag cgacgaggac gacgagcagc gccgacccga
      661 tgagaaggga cgcgccgccc gatccgtcgc cggcgcgggc ctctccgacc gcgcccgacc
      721 agagcgcgaa ggctgcgacc gcgactccca gcacgagaac ccggtacgac aaggtcgccg
      781 acacgcttgc cagggtcccg gcgatcgcgg cgacggtgaa gagacctgcg gccgtcgccc
      841 acgtccgccg ccgcgcgggg ctcgtctgcc gggaaccggt gagagaaagt gcagagagga
      901 cgagagccgt cagcccggca acccgcggga gcgcaacgag ggactggacg actccttcgc
      961 cgagttcggg agtctcgagc cgcgtcgcga cgaagagcga gattccgaag agcaggactc
     1021 cggcgaggag cgcgatcgga gtcggagacg tatgcggacg gccggcgaac gagaccagac
     1081 ggcctggcaa cacggccaac ccgagagcga gacccgccac cgtcaggaaa gagagaggcg
     1141 aggccggtcc gaaccgggcc tcgacggcga ggtacgcgac cgcgagcgcc agccccgcgc
     1201 gcaacgggtc ccgcagacgg ccggcgagag cgacggcgat gacgagcaga gcggcgaacc
     1261 cgaagttccg aagccttgcg acgtcggtac cggccgccgg gcgcgcggtg acgagaacgg
     1321 tgcccgatga gtccgtgagg agaggcgacc ctcccgtcga ggcgggcctc caggccgcgg
     1381 aagcgccctg cagtccgagc gcccgggcga agtcgggcgc atcgaccggg aacgactgcg
     1441 aggcgtagac gacgccggag aatccgccgt cgccgacctg gcggcgctgg acggccgtca
     1501 gtcgggtggc cgaccagcga accgcgaggc cctccggcga gaacgtgagg ttctcgagcg
     1561 acggcgcgtc gccccaccac gcatggacga ttccggacgc atccgtcaag gccagaccgg
     1621 cgccgggcgg cgatgaggcg agcgagtgac ccgcggcatt gaagagcgca aggcgattga
     1681 cggcaatccc gccgccctcg agggaccggg ccacctcggg ctgtgactgg aagtccgagg
     1741 cgcggcgcgt gaggtcgccg acggcgtcat ggaaggcgcg ctggagcgac gtcagagccg
     1801 cggacctcgc gcgagggccg atcgggagcg gcaggcgtcc gtagagaaag aggagaaagg
     1861 gcgtggcgag gaccgccccg gcgagcggga cgcgcaggcg tcggatcacc ggagcgtcgg
     1921 cagatccact ccgcgctccc tcgcggcggc gatcgcctcg ggatatccgg cgtctgcgtg
     1981 gcgcattacc ccggtgcccg ggtcggccgt caggacgcgc tggagtcttt tcgccgctcc
     2041 gtcggtcccg tccgcgacga cgaccatgcc cgcgtggagc gagtagccga tgccgacgcc
     2101 gcctccgtga tggaccgaaa cccacgtcgc gccgcacgcc gtgttgagca gggcgttcag
     2161 gatgggccag tcggcgatgg cgtccgatcc gtcccgcatc ccctcggtct ctcggttcgg
     2221 cgatgcgacg gacccggcgt ccaggtggtc ccgcccgatc acgatggggg cgcgaacccg
     2281 gcctcgccgg acgaggtcat tgaagagaac gccggcccgg gcccgctcgc cgtatccgag
     2341 ccagcagatg cgcgccggga gaccctggaa ggcaacgcga tccttcgcga gggtgagcca
     2401 tctccggagc gcggcgttct ccgggaagag atcgaggagc gcgcggtcgg tctccgcgat
     2461 gtcctgcgcg tcgccggaga gcgcggccca tcggaagggg cctttgcctt cgcagaacag
     2521 cggacgaatg tagagcggga cgaatcctcc aatgtcgaac gcattttcga cgccggcctt
     2581 cttcgcctgc ccccgcaggt tgttgccgta atcgaagacg acggcgccgc gccgtttcat
     2641 ctcgagcagc gcccgcacgt gggccgccat cgtctcgtac gaacgccgca cgtacgtcac
     2701 cgggtcttcc cgccgcaggg cgattgcctc ctcgagcgac aggttccgag ggacgtagcc
     2761 gtcgagctcg tcgtgcgcgg aggtctggtc cgtgacgagg tcgggcacga tgccgcgcgc
     2821 gacgaggcgc tcgaggagat cgacggcgtt aacctggatg ccgatcgaca ccgcttcccg
     2881 ccggtcgcgc gattccaaag cgcgatcgac cgcgcggtcg agcgagtctt cgcgaacgtc
     2941 gagatatcgc gtggagagcc gtcgttcgat tcggtggccg tccacttcgg cgatgagcgc
     3001 cgtcgcaccg ttcatcgtcg cggccagcgg ctgcgcgccg cccatgccgc cgagccccgc
     3061 ggtcacgacg agtcggcccg agaggtcccc gctgaactcc ttttcggcgg cggccgcgaa
     3121 ggtctcgaac gtgccctgca ggatcccctg ggtcccgatg tagatccagg agcccgcggt
     3181 catctgcccg aacatcgtga gcccggccga ctcgagccgg cggaactcgt cggccgtcgc
     3241 ccacgcgggg acgaggaggg cgttggcgat cagcacgcgc ggcgcgtcct cgtgggtttc
     3301 gaagacgccg accggcttgc ctgattggac gagaagagtc tcgttggaag agagccgctt
     3361 caacgtctcg acgatcgcgt cgaagcattc ccagttgcgc gccgccttcc cggtcccgcc
     3421 gtagacgatg agctcgtccg gtttctcggc gacctcggga tcgaggttgt tttcgagcat
     3481 gcggagcgcg gcttcctggg cccagccccg gcaacgcagg tcggtccccc gggccgcgcg
     3541 aacgacccga ggtcccgcaa cgacggcagg cacggaggga attctacgcg agggactcga
     3601 cgagcttcga aatggcgccg ccgtcgagga acgcccgggc cgcggcgagg tcgggcgcga
     3661 gctcgcggtc ggagctccac ggcgcgacga tcgtgcggaa gcgcgtcctc acacgctcga
     3721 tcgggccgga ggaggcgagc ggacgcagga actccatcgc ctggaatgcg accatgagct
     3781 cgatcgcgag caccatggcg aggttttcga cgattgccgc gagcttccgg gcggcgatcg
     3841 gcgacatcga gacgtggtct tctttcgcgg cggaggtcgg aatcgagtcg accgacgcgg
     3901 ggtgcgcgag cgctttgcac tccgacacca gagccgcggc cgtcacctgc gccatcatga
     3961 gcccggagtt caggccccct tcctcgacga ggaaggccgg gaggtccgag agagccgggt
     4021 tgacgagctt ctcgatgcgc cgctcggaga tcgacgcgag gtctgcggca gcgatcgcgg
     4081 catagtcgag gacgaggccg accggcgcgc cgtggaaatt tccgcccgag acgacatctc
     4141 cccgggcggc gaatacgagc gggttgtccg tcgcgctgtt catttcccgt tcgaggacgt
     4201 cgcgcgcatg gtcgaacgcg tc
//
LOCUS       DASWAV010000617         4193 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U2665
            k141_476463, whole genome shotgun sequence.
ACCESSION   DASWAV010000617 DASWAV010000000
VERSION     DASWAV010000617.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960316
            Sequence Read Archive: SRR10260007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 4193)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4193)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:47:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,929
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4193
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_476463"
                     /isolate="SMAG_U2665"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.36444 N 132.31889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..348)
                     /locus_tag="VF958_12795"
     CDS             complement(<1..348)
                     /locus_tag="VF958_12795"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX9150033.1"
                     /translation="MKRGKRRTAVQRKRGSLDWVRKRPLPIVSVLIADRDKATRAAAT
                     RVIQSLRGMRIVGEAKSGLEAVSATARLKPRVVLLDLKLSSEFGATLVSVLRRKSART
                     RVILLVGRAPEAKI"
     gene            complement(345..1022)
                     /locus_tag="VF958_12800"
     CDS             complement(345..1022)
                     /locus_tag="VF958_12800"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX9150034.1"
                     /translation="MSDRARAASVVLIASPIAGVRGQWSQGLQEKFPLYEVAERSALE
                     RSMANLKPAVLLLDLALPGLGGVEGVSAVQRISPLTSIMLLTSTPNDREGLAALKGGA
                     RGYCSSETDPALLRKAVTVVLKGEIWVARNVYAPLLEELTSLTERRHKEFPPNLDTRL
                     DRLTPREREIAQLIGGGASNKEIAGRLNITEATVKAHLTAIFRKLGLSDRLGLALFVT
                     EYNRVTR"
     gene            complement(1140..>4193)
                     /locus_tag="VF958_12805"
     CDS             complement(1140..>4193)
                     /locus_tag="VF958_12805"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF004225.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="error-prone DNA polymerase"
                     /protein_id="HEX9150035.1"
                     /translation="AVALVDTNGVYGAPRFYKAAQQAGIRALVGAEVALEGERRNPEI
                     GNRKSREPENFPRLTVLVENREGYKNLCRLITSGALGRAKGQGRVSLDEVAAHAEGLH
                     CVTGGQQSPVGRALASGGPDSAHQLLEKLGAIFTGRLHVELQRHGLRGEEHGNRALAD
                     LAARLRLPLVATNGVRYARARDKDLHDVMTALRHKTDLDRAGALLAGERERHFKGAAE
                     MADLFADFPRALDGAWELSERLDFTLADLGYRFPDYPLPPGETPSSYLRRIAWNGARS
                     RFRPLTARAQAQIAKELDLIEKLDLAGYFLIVWDIVQFCLKEKILVQGRGSAANSAVC
                     YALSITAVDPVKMELLFERFLSEERGEWPDIDLDLPSGDQREKVIQHVYEKYGARGAG
                     MTANVITYRDKMSAREVGKALGYSPEQVDRLSKQLGSWNFGEIREPIDELTKEVAAAG
                     LDPREPRSRHFMRLWLEIQNRPRHLGQHSGGMVIAAGRLDEVVPLEPASMPGRVVVQW
                     DKDDCADMGMVKVDLLGLGMLAALEEALPMIRTNEKVEVDLAHLPPDDPAVYEMLNKA
                     DTVGLFQVESRAQMASLPRHAPKVFYDIVVQVAIIRPGPIVGGMVHPFFDRRRGLAPV
                     EYPHPCLEPILKRTLGVPLFQEQLLKIAMVAAGFTGGEAEELRRAMGFKRSQERMEAI
                     EAKLRRGMTERGIDGAAQEQIVKSITSFALYGFPESHAASFALIAYASAYLKAHHPAA
                     FYAAILNSWPMGFYHPATLVKDAQRHGTRMLPIDVARSGWKCRWEGGAVRLGLRFVGG
                     LREATGKRIEAEQSVALFRDADDLARRCGLREDELVRLAYAGALGAFGRTRRGALWQV
                     AKVARPSGPLFTGMATDDPSPLPDMSPREETAADYEATQMTTGPHLVEHLREEFRRKG
                     VLSAAELWKLPHGAFVRAGGAVIVRQRPGTAKGFVFLTLEDETGMCQAIVRPDLFREY
                     RTVIVGSGTLVVEGRLQKLDGTLSVKGERFWGVSQLEEVPSHDFH"
BASE COUNT          653 a         1472 c         1380 g          688 t
ORIGIN      
        1 gatcttcgcc tccggtgcgc gcccgacgag gaggatgacc cgcgtgcggg cgctcttgcg
       61 ccgcaggacg gagacgagcg tcgctccgaa ttccgaggag agcttcaggt cgagcagtac
      121 gacacggggc ttcaaacgcg ctgtcgccga aaccgcctcg agccccgact tcgcttctcc
      181 gacgatccgc atcccgcgca gcgactgaat gacccgcgtc gcggcggctc gggtggcctt
      241 gtcgcgatcg gcgatcagga cgctcacgat cgggagcggt cgcttcctca cccagtcgag
      301 gctgcctcgc ttgcgctgaa cggccgtgcg ccgctttcct cgtttcaccg cgtcacccgg
      361 ttgtactcgg tgacgaagag cgcgagaccg agacggtccg agagtccgag ttttcgaaag
      421 atcgcggtca gatgcgcctt cacggtggcc tccgtgatgt tgagccgacc cgcgatctcc
      481 ttgttgctcg ccccccctcc gatgagttga gcgatctcgc gctccctcgg ggtcaaccgg
      541 tcgagccggg tatcgagatt cggcgggaac tccttgtgtc tccgttctgt cagtgacgtg
      601 agctcttcga ggagcggagc gtagacgttc cgggcgaccc agatctcgcc cttcaggacg
      661 accgtcaccg cttttcgcag gagtgccgga tccgtctcgg aactgcagta gccgcgcgcc
      721 ccgcccttca gcgcggcgag tccctcgcgg tcgttgggag tgctggtcag aagcatgatc
      781 gacgtgagcg gggagatccg ctggacggcc gaaacgcctt cgactcctcc caggcccgga
      841 agcgccaggt cgagtagcag aaccgcgggc ttcagattgg ccatgctgcg ttcgagcgcc
      901 gaccgctccg ccacctcgta caggggaaat ttctcctgca ggccctgaga ccactggccg
      961 cgcacacccg caatcgggct ggcgatcagg acgacggaag cagctcgagc tctatcagac
     1021 atgaatccaa gaaaactgcc gacaagctac aggggcgggg gcggagtgtc aatgagaacg
     1081 gtcgttcgcg gctcgtggcg aaacggagtc ggcaccgacg aaagtcgagg aaaggttgat
     1141 caatggaagt cgtgactcgg gacttcctcg agctgactga ccccccagaa cctctccccc
     1201 ttcaccgaca gcgttccgtc cagcttctgc aaccgtccct cgacgacgag cgtcccggat
     1261 ccgacgatga ccgtccggta ctcgcggaag aggtcgggtc ggacgatcgc ctggcacatc
     1321 ccggtctcgt cctcgagcgt caggaagacg aaacccttcg cggtgccggg ccgctgacgg
     1381 acgatgaccg cgccccccgc ccggacgaac gcgccgtgcg ggagcttcca gagttccgcc
     1441 gcggaaagca cccccttgcg tcggaactcc tcccgcaggt gctcgactaa gtgcggcccc
     1501 gtcgtcatct gggtcgcttc gtagtcggct gccgtctcct cgcgcgggct catgtcgggc
     1561 agcggcgacg gatcgtcggt cgccattccc gtaaagagcg gccccgacgg ccgcgcgacc
     1621 ttcgcgacct gccagagcgc gccgcggcgc gtgcggccga acgcgccgag cgcgccggcg
     1681 tacgcgagac gcacgagctc gtcctcgcgg agcccgcatc gccgcgcgag atcgtcggcg
     1741 tcgcggaaga gcgcgaccga ctgctccgct tcgatcctct tccccgtcgc ctcacgcagt
     1801 ccgccgacga accgcagccc cagccgcacc gcgcccccct cccatcggca cttccatccc
     1861 gagcgtgcga cgtcgatcgg caacatccgc gtgccgtgcc gctgcgcgtc cttcacgagg
     1921 gtcgcggggt gatagaagcc catcggccac gagttcaaga ttgccgcgta gaacgcggcg
     1981 ggatggtgcg ctttgagata cgcgctcgcg taggcgatca gcgcgaacga cgccgcgtgc
     2041 gactcgggga agccgtacag cgcgaacgac gtgatggact tgacgatctg ctcctgcgcg
     2101 gcgccgtcga tcccgcgctc cgtcatcccc cggcgcagct tggcctcgat cgcttccatc
     2161 cgctcctgcg agcgtttgaa ccccatcgcg cgccgcagct cctccgcctc gccgcccgtg
     2221 aaccccgctg cgaccatcgc gatcttcaag agctgttcct gaaagagcgg gacgccgagc
     2281 gtgcgcttca ggatcggttc gaggcacgga tgcgggtact cgacgggcgc gagaccgcgc
     2341 cgccggtcga agaacggatg caccatcccg ccgacgatcg gccccggccg gatgatcgcg
     2401 acctgtacga cgatgtcgta aaagaccttc ggcgcgtgcc gcggcagcga cgccatttgc
     2461 gcccggctct cgacctggaa gagaccgacc gtgtcggctt tgttcaacat ctcgtaaacg
     2521 gcggggtcgt cgggcggaag gtgcgcgagg tcgacctcga ccttctcgtt cgtccggatc
     2581 atcgggagcg cttcctccag cgccgcgagc atgccgaggc cgagcaggtc caccttcacc
     2641 atccccatgt cggcgcagtc gtccttgtcc cactggacga cgacgcgtcc gggcatggac
     2701 gcgggctcga gcggcacgac ctcgtccagc cgccccgcgg caatcaccat cccgccggag
     2761 tgctgcccga gatggcgcgg ccggttctgg atctcgagcc acaggcgcat gaagtgtcgc
     2821 gaccgcggct cgcgcgggtc gagccccgcg gcggcgacct ccttcgtgag ctcatcgatc
     2881 ggctcgcgga tctccccgaa gttccagctc ccgagttgct tcgaaagccg gtcgacctgc
     2941 tcgggcgagt agccgagcgc cttgccgact tcgcgggcgg acatcttgtc gcggtacgtg
     3001 atgacgttcg cggtcattcc cgcgccgcgc gcgccgtatt tctcgtagac gtgctggatg
     3061 accttctcgc gctggtcgcc ggagggcagg tcgaggtcga tgtcgggcca ctcgccgcgc
     3121 tcctccgaga ggaagcgctc gaagaggagc tccatcttca ccggatccac tgccgtgatc
     3181 gagagcgcgt agcacacggc gctgttcgcg gcggagccgc gcccctgcac gaggatcttt
     3241 tccttcaggc agaactgcac gatgtcccag acgatcagga agtagccggc gaggtcgagc
     3301 ttctcgatca ggtcgagctc cttcgcgatc tgcgcctgcg cgcgggcggt gagcggccgg
     3361 aagcgcgatc gcgcgccgtt ccacgcgatc cggcgaaggt aggacgaggg cgtctcgccc
     3421 ggcggaagcg ggtagtcggg gaaccggtaa ccgaggtcgg cgagcgtgaa gtcgagccgc
     3481 tccgacaact cccacgctcc gtcgagcgcg cgcggaaagt cggcaaagag atcggccatc
     3541 tccgcggcgc ccttgaagtg ccgctcacgc tctcccgcca gcagggcgcc cgcgcggtcg
     3601 aggtcggtct tgtggcgcag cgcggtcatc acgtcgtgca gatccttgtc gcgcgcgcgc
     3661 gcgtagcgca cgccgttcgt ggcgacgagc ggaagacgca gtcgcgcggc aaggtcggcg
     3721 agcgcgcggt tgccgtgctc ctcgccgcgg aggccgtgac gctgcagctc gacgtggagc
     3781 cggccggtga aaatcgcgcc gagcttctcg aggagttgat gggcggagtc gggcccgccg
     3841 ctcgcgagcg cgcgcccgac aggactctgc tgcccgccgg tgacgcagtg gagcccttcc
     3901 gcgtgcgcgg cgacctcgtc gagggagacg cgcccctgcc ctttcgcgcg cccgagcgct
     3961 ccgctcgtga tcaatcggca gagattcttg taaccctcac ggttctcgac gagcacagtg
     4021 agccggggga agttctccgg ctccctcgat ttccgattcc cgatttccgg gttccgacgc
     4081 tcgccttcga gcgccacttc cgctcccacg agcgcccgga tccccgcctg ctgcgccgcc
     4141 ttgtagaacc gcggcgcccc gtagacgccg ttcgtgtcga cgagcgcgac cgc
//
LOCUS       DASWAV010000618         3908 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U2665
            k141_517704, whole genome shotgun sequence.
ACCESSION   DASWAV010000618 DASWAV010000000
VERSION     DASWAV010000618.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960316
            Sequence Read Archive: SRR10260007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 3908)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3908)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:47:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,929
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3908
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_517704"
                     /isolate="SMAG_U2665"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.36444 N 132.31889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1658)
                     /locus_tag="VF958_12810"
     CDS             complement(<1..1658)
                     /locus_tag="VF958_12810"
                     /inference="COORDINATES: protein motif:HMM:NF020037.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX9150036.1"
                     /translation="MSVAVPQTFRRESRLFLWVALLLVLFLNLLTLLFFRRAVDWANQ
                     SLERRAAEILRRIPLSAGEADDATSAALGKAALERDVLYVAAYDRQGRRVRAFGPEMD
                     APESLPTFVPAPEQIAFAWRKNPTLLVATRGTAHGSISMALDPGPGAALRSLVRALTL
                     IVPIAGAALVVLAGFYLRSLLSPYDRLIAAAGSAPAGSPSGIAETTDEREFLIGRFEA
                     TIAALRDKERELRARADDLETAARVLSRNLPTGLLSIDSRGTIVELNEAGREILRISD
                     DLHGRRFDEALSAVPEFRDVLSRVLSDRNPVGRREIHWNSSEEMRSFGVTVTPAEGAD
                     GRFLGVLALFTDLTEMRRLEARIALTRHLADLGQVSAGAAHEFRNAAAAIDGFADLAL
                     RSPDRAAEHLRAIRREAQEMSRVTNDFLLFARPESFVPEPVDLTAVAAAAAAETESAF
                     RGVRVVLDGAFPIVPGSEVLLRRALVNLLRNAVEATPAERRGEPSAILLTAGTGDGEV
                     LLSVGDRGAGVDASLREEIFLPFYSTKPRGSGFGLAIVARIIEL"
     gene            complement(1655..2455)
                     /locus_tag="VF958_12815"
     CDS             complement(1655..2455)
                     /locus_tag="VF958_12815"
                     /inference="COORDINATES: protein motif:HMM:NF013632.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HEX9150037.1"
                     /translation="MTAFDLGFGSGSPFVAVAVFLFGLVIGSFLNVVIHRLPRGESVA
                     FPGSHCPACRAPIRAYDNVPVVSWLLLRGRCRVCRAPISVRYPVIELTNGFLWIGVFL
                     RAPGWADFATGAFLCSACIALLAIDAEFRILPDRITLTGIVVGIALSFFSHMRTPLSA
                     IGGAALGAGGLFLLAFLWEKLRGVEGMGMGDVKMLGMVGALLGAPGVVVTVLLASVTG
                     SLVGLALILVRRGSLQTALPFGVFLALGAVAAFFWGPALVDLYRGALR"
     gene            complement(2452..>3908)
                     /locus_tag="VF958_12820"
     CDS             complement(2452..>3908)
                     /locus_tag="VF958_12820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9150038.1"
                     /translation="RGPLPVAEREPGAIPIPALAAAFARPPGATRTYWLPSLFVGPLA
                     LALAAAAFFEKERRPLAALLLAFGIAGLLIAAAGPPGSWLRAIPPLDRIRYPEKALSA
                     TIFAVAALAGLGLDTLRFRAGGRILRLVFPLLAAGTVLLLFAVPSSPELRAAAVIGLF
                     PAVALALGAGRRETAGAFLGGFAALAIVATYAIANRGLFTFVDEGQIRRRPPSLDALG
                     SLPGRVLTPPAQALSNWIVRDASYDAATVAREREALAGYTNLLFGISTVRTAAALPAA
                     GARAIADSIDAAADPVHAAGPVSARLLWTPFRPGPVPSRKVGEFFRAPIAPYRPRLSF
                     VRSYRVEPSAPRAWMRAAAGEIDVSREVLLDREPMPAPPAGARSPILLARLAEDLPEK
                     VVAEIATNTPGVLVMTDLHYPGWIAEESGRRLEILRADGFFRAVALPAGSHRVTFRYR
                     PLSVLVGAAVSAAALLMVLLLAYQGEPVRVGRRP"
BASE COUNT          702 a         1306 c         1468 g          432 t
ORIGIN      
        1 tgcagttcga tgatccgggc cacgattgcc agaccgaagc cggagccgcg cggcttcgtc
       61 gaatagaacg gcaggaagat ctcttcccgg agcgacgcat ccacgcccgc tccgcggtcc
      121 ccgacggaga gcaggacttc cccgtcgccg gtcccggccg tgaggaggat cgcggacggt
      181 tccccgcggc gctccgccgg agtcgcctcc acggcgttgc gcagcaggtt gacgagcgcg
      241 cggcgcagca gcacctcgga tcccggcacg atggggaatg ctccgtcgag cacgacgcgg
      301 accccgcgaa atgcgctctc cgtctcggcg gcggcggcgg cggccaccgc cgtcaggtcg
      361 accggctccg gaacgaaact ctccggccgc gcgaagagca gaaagtcgtt cgtcacacgg
      421 ctcatctcct gcgcctcgcg acggatcgcg cgcaggtgct ccgccgcccg gtcgggcgag
      481 cgcaacgcga ggtctgcaaa gccgtcgatc gcggcggccg cgtttcgaaa ctcgtgcgcg
      541 gcgcccgccg agacctgtcc caggtccgcg aggtggcgcg tcagcgcaat tcgcgcctcg
      601 aggcgacgca tctcggtcag gtcggtgaag agcgcgagga cgccgaggaa gcgtccgtcc
      661 gcgccttcgg cgggagtcac ggtcaccccg aaagagcgca tctcctccga cgagttccag
      721 tgaatttcgc ggcgccccac ggggttgcgg tcggaaagaa ccctggagag gacgtcgcgg
      781 aattccggca ctgccgaaag ggcctcgtcg aatcgccgtc cgtggaggtc gtcggagatc
      841 cgaaggatct cgcggccggc ttcgttcagc tcgacgatcg ttcccctcga atcgatcgag
      901 aggaggccgg tcggcaggtt gcgcgagaga acgcgcgccg ccgtctcgag atcatcggcc
      961 cgggcgcgga gctcgcgctc cttgtcgcgc agagccgcga tcgtcgcctc gaatcggccg
     1021 atcaggaact cccgctcgtc ggtcgtctcg gcgatgccgg acggcgagcc ggctggagcg
     1081 cttccggccg ccgcgatcag gcggtcgtag ggcgagagca gcgaacgcag atagaagccc
     1141 gcgagaacga cgagcgccgc accggcgatc gggacgatca gcgtcaacgc tcgcacgagc
     1201 gatcgcaggg cggcgcccgg accggggtcg agagccatcg agatcgaacc gtgggccgtg
     1261 ccccgggtcg cgacgagcag agtcgggttc ttccgccacg cgaacgcgat ctgttcgggg
     1321 gcaggcacaa aggtcgggag ggattccggc gcgtccatct ctgggccgaa cgcgcggacg
     1381 cgccgaccct gccggtcgta agccgcgacg tagaggacgt ctcgctcgag cgcagctttc
     1441 ccgagcgcgg cgctcgtggc gtcgtccgcc tcgccggcgg atagcgggat gcgccggagg
     1501 atttcggcgg cccggcgctc gagcgactgg ttcgcccagt cgaccgcacg acggaagaac
     1561 aggagcgtca ggagattcag aaacaggacg aggagcagcg cgacccagag gaacagccgg
     1621 gattcgcgtc ggaacgtctg cgggacggcg acgctcaccg gagggcccct cgatagagat
     1681 cgacgagcgc gggcccccag aagaaggcgg cgaccgcgcc gagcgcgagg aagaccccga
     1741 agggcagcgc ggtctggagc gagccgcggc gcacgaggat gagcgcgagg ccgacgaggc
     1801 tgcccgtgac cgaggcgagg aggaccgtca cgacgacgcc gggcgcgccg agaagggcgc
     1861 cgaccatccc gagcatcttg acgtcgccca tccccatccc ctcgaccccc cgtagcttct
     1921 cccagaggaa tgcgaggagg aagaggcctc ccgcccccag ggcggcaccg ccgatcgccg
     1981 agagcggcgt gcgcatgtgc gaaaagaagg agagcgcgat tccgacgacg attccggtca
     2041 gcgtgattcg atccggaagg atgcggaact ccgcgtcgat cgcgagaaga gcaatacagg
     2101 ccgagcagag gaaggcgccc gtggcgaaat ccgcccagcc gggggcgcgc aggaagacgc
     2161 cgatccagag gaacccgttg gtgagctcga tcaccggata acgaaccgat atcggcgcgc
     2221 ggcatacgcg gcatcgcccg cgcagaagga gccacgagac aaccggcacg ttgtcgtacg
     2281 cacgaatcgg agcccggcac gccgggcagt gagagccggg gaacgccacc gactcgccgc
     2341 gaggcagccg gtgaatgacg acgttcagaa agctcccgat gacgaggccg aagagaaaga
     2401 cggcgacggc gacgaagggc gacccggagc cgaatccgag gtcgaaggcc gtcacgggcg
     2461 gcgtccgacc cggaccggtt caccctgata ggcgagcaac agaaccatga gcagcgcggc
     2521 ggcgctcact gcggcgccga cgagaaccga gagcgggcgg tagcggaacg tcacgcggtg
     2581 ggatcccgcg gggagcgcga cggcgcggaa gaacccatcc gcccgcagga tctcgagacg
     2641 gcgcccgctt tcctcggcga tccagcccgg gtagtggaga tccgtcatga cgaggacgcc
     2701 cggggtgttc gtcgcgatct ccgcgacgac tttctcggga aggtcttccg cgaggcgggc
     2761 gaggagtatc ggcgaacgcg cgccggcggg cggcgcgggc atcggctcgc ggtcgaggag
     2821 gacttctcgg gagacgtcga tctctcccgc ggccgcgcgc atccaggcgc gtggcgcgct
     2881 cggttcgacc cggtagctgc ggacgaatga gagccgcggg cgatagggcg cgatcggagc
     2941 gcggaagaat tctccgacct tgcgcgacgg gacgggcccc ggccggaaag gggtccacag
     3001 gaggcgcgcg gagaccggcc cggccgcatg gaccggatcc gcggccgcgt cgatcgagtc
     3061 cgcgatcgcg cgggcgccgg ccgccgggag ggcggcggcc gttcggacgg tcgaaatgcc
     3121 gaagagcaga ttcgtgtatc cagcgagggc ctcccgctcg cgcgcgaccg tcgccgcgtc
     3181 gtagctcgcg tcgcgaacga tccagttcga gagcgcctgc gccggcggag tcagcacgcg
     3241 gccgggcagg ctcccgagag cgtcgaggga gggcggacgg cggcggatct ggccctcgtc
     3301 aacgaaggtg aagagaccgc ggttggcgat cgcgtacgtc gcgacgatgg ccagcgctgc
     3361 gaagccgccg agaaaagcgc cggcggtctc gcggcgtccc gccccgagcg cgagcgcgac
     3421 cgccggaaag aggccgatga ccgcggcagc gcggagctcg ggggaagacg ggacggcgaa
     3481 caggaggagg acggtccccg cggcgagcag gggaaaaacg agccgcagga tgcgaccgcc
     3541 ggcccggaat cggagcgtgt cgagacccag cccggcgagc gccgcgacgg cgaaaatggt
     3601 cgcggagagc gccttctccg gatagcggat ccggtcgagc ggcgggatcg cgcggagcca
     3661 cgacccggga ggccccgcag ccgcgatcag gagtcccgca atcccgaagg cgagaagaag
     3721 cgccgcgagc gggcgccgct ccttttcgaa aaacgccgcg gccgccagcg cgagggcgag
     3781 cggcccgacg aagagtgacg gcagccagta cgtccgcgtc gcacccggcg gccgcgcgaa
     3841 cgccgcggcg agcgccggaa tcgggatcgc gccgggctcc cgctcggcca cggggagcgg
     3901 cccgcgac
//
LOCUS       DASWAV010000619         5757 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U2665
            k141_75024, whole genome shotgun sequence.
ACCESSION   DASWAV010000619 DASWAV010000000
VERSION     DASWAV010000619.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960316
            Sequence Read Archive: SRR10260007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 5757)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5757)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:47:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,929
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5757
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_75024"
                     /isolate="SMAG_U2665"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.36444 N 132.31889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..676)
                     /locus_tag="VF958_12825"
     CDS             complement(<1..676)
                     /locus_tag="VF958_12825"
                     /inference="COORDINATES: protein motif:HMM:NF013935.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit D"
                     /protein_id="HEX9150039.1"
                     /translation="MTAAATRARWLELAGEQRAAREAAELLDQKREVLLRELHRRLAA
                     EAATRLRAAAALERARIALDDACVELGGPAVEAASLAVSDAPSLEARPRSLLGIALPD
                     LGGSLPAPRPSWSPGGTSESLDLSRALFLEVVPILVELARERLAVSNLQRGLARTNRR
                     WNALDSVVLPGLARDLAAIAGALAEEERDDDFRRRRRKASGFGKPGRDNAPPSRYSTT
                     STFSPRT"
     gene            complement(673..2070)
                     /locus_tag="VF958_12830"
     CDS             complement(673..2070)
                     /locus_tag="VF958_12830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007043707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit B"
                     /protein_id="HEX9150040.1"
                     /translation="MSRGTELEYRGLAALRGPLGFVTGIAGASYREHVEVIGPDGAIR
                     VGRVVGISEDTAVLEIFSGSDGLALDACRVRFRGEPLRFGVGPELVGRVFDGTGRPRD
                     GLPPPAAVEQRSIEGSPINPVRRDVPGEFLETGVSAIDGLNALVLGQKLPIFTESGLP
                     HDALAMQIVRQARAPGVGEFAIVFAALGLPRETAFRYLEDFRASGALSRVVAFFNLAD
                     DPAPERLITPRCALTAAEYLAFDRGFHVLVILNDVTNYGEALREVASSAGEVPGRKGY
                     PGYLYSDLASIFERAGRVRGRPGSLTQLPIVTLPSGDVTHPIPDLTGYITEGQIVLQR
                     SLDRRGIYPPVDVLPSLSRLMSGGIGAGRTHEDHPALARQLYAACARAARARSLESII
                     GREELSETERSYLEFGETFEREFLSQGAEERRGIVETLDRAWTALARLPDSELGRLPA
                     RLREARRAAASPVAS"
     gene            complement(2067..3878)
                     /locus_tag="VF958_12835"
     CDS             complement(2067..3878)
                     /locus_tag="VF958_12835"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012633861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit A"
                     /protein_id="HEX9150041.1"
                     /translation="MREDETGDRPCGRIVGISGSVVRAETTSPVSMGEVVRVGEDRLA
                     GEVIALEGNRATAQVYEETAGLAAGDPFFATGSPLTVELGPGLLGSVLDGLARPLAAL
                     AAAEGDFLGRGRSLPALDRDRAWGFEPRVAPGASVVAGSLLGVVPETRAIEHRVLVPP
                     GIAGRLLEIAPTGSRRVADSVARIETATGTVTLPLFQRWKVRAPRPFAEALPLSIPLL
                     TGQRVLDTFFPLPRGGAAGMPGGFGTGKTITQQQLCKWMRADVIVFVGCGERGNEMAQ
                     VLHELPELADPRTGWTLAERTVLLANTSNMPVAAREASLYTAVTIAEYYRDMGYHVAV
                     LADSTSRWAEALREISGRLEEMPAEEGYPAYLATRLAAFYERAGRVRTLSAAEGSVSI
                     VSAISPPGGDLTEPVTRHTRRFTRCFWSLDPRRAEARIFPAIGLEESSGDASPAVRSW
                     WEAETVPEWGRLRREAIALLEKAAQLERMARLVGTESLPERERFALRMGSLFQEGYLA
                     QNAFEPKDASCSPARQARLLRLLLRVRDLGLEAIERGATSREIEAMPVLSRIERAKEE
                     IGDDELPRLADLETELEAAFSSLPVALSSPEARAEAG"
     gene            complement(3875..4477)
                     /locus_tag="VF958_12840"
     CDS             complement(3875..4477)
                     /locus_tag="VF958_12840"
                     /inference="COORDINATES: protein motif:HMM:NF014093.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033178 - proton-transporting two-sector
                     ATPase complex, catalytic domain [Evidence IEA];
                     GO_function: GO:0046961 - proton-transporting ATPase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit E family protein"
                     /protein_id="HEX9150042.1"
                     /translation="MKTLGSPASVADAVREEASREVERLLRDTDEEIVRLRSEAQSGD
                     VTPPDRPARLAAARREVSARLAREDWEDRRAALEERERWTERAIRLARERLDGDREAA
                     SRRELLLALAVEAAERLPAGAVEISVSASDASIADDAFVEELARRARRTVARARGDLP
                     QSGCVVSTVGGKVRFDNSLEARARRFEPLWRAALTEIYGP"
     gene            complement(4474..4734)
                     /locus_tag="VF958_12845"
     CDS             complement(4474..4734)
                     /locus_tag="VF958_12845"
                     /inference="COORDINATES: protein motif:HMM:NF014092.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit F"
                     /protein_id="HEX9150043.1"
                     /translation="MRIRLVGDADDALGFSLAGIEGMIPRDRREADRAFAAIEAEKGI
                     GLVFVSAAVEDLGSPALERLRLRGGIPAIVVLPARARGDTSP"
     gene            complement(4731..5054)
                     /locus_tag="VF958_12850"
     CDS             complement(4731..5054)
                     /locus_tag="VF958_12850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007195570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase subunit C"
                     /protein_id="HEX9150044.1"
                     /translation="MKRRITIALAAALSPLLVLPLFAAQVPASAAPASTELAKWGFIA
                     AALVTGLAAIGAAIAVAVVGSAAVGALAEKPETAGRALIFVGLAEGIAIYGLIVAIMI
                     LGRLP"
     gene            complement(5051..>5757)
                     /locus_tag="VF958_12855"
     CDS             complement(5051..>5757)
                     /locus_tag="VF958_12855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9150045.1"
                     /translation="WMDPLRDLTTFLELAIALGVVLVSGGLVLNAVNAWRSGERSLAV
                     FGPRGLLGAFGYWVFAALAARAFVGGKVHLPAAAIFALLAIPLALILLRRPIVRWLEK
                     GHPAPRRAGVEKSPFFFAALEGSVELVDAVVSYFANTISFLRVAAFAMVHAGAFLALF
                     ALADTLSRLRGGGALSILLLVAGNIVMIFLEGLTVSVQVLRLEYYEFFGKFFRGGGEP
                     YRPLMLRPRLAKGESP"
BASE COUNT          918 a         2038 c         2045 g          756 t
ORIGIN      
        1 acgttctcgg ggagaacgtc gaggtcgtcg agtagcgact cggcggggcg ttgtccctgc
       61 ccggttttcc gaagcccgac gccttccgcc gacgccggcg gaagtcgtcg tcacgctcct
      121 cttcggcgag cgccccggcg atagcggcca ggtctcgcgc cagcccgggc agaacgacgc
      181 tgtcgagtgc gttccacctc cgattggtcc gggcgaggcc acgctggagg tttgacaccg
      241 ctagccgctc gcgggcgagt tcgacgagaa tcgggacgac ttccaggaag agggcgcgcg
      301 agaggtcgag cgattcggac gtcccgccgg gagaccagct cggacgcggc gccggaaggc
      361 tgcctccgag gtcgggcagc gcgattccga ggagtgaacg cgggcgcgcc tccagcgagg
      421 gggcgtcgga caccgcgagc gacgcggctt cgaccgcggg gccgccgagc tccacgcacg
      481 cgtcgtccag cgcgatgcgc gccctctcga gcgccgctgc cgcgcggagg cgcgtcgcgg
      541 cctccgccgc gagccggcgg tggagctcgc ggagaagtac ttcccgcttc tgatcgagga
      601 gctcggcggc ttcgcgcgcc gcgcgctgct cccccgcgag ctcgagccag cgcgcccggg
      661 ttgccgccgc cgtcacgatg ccaccggaga agctgcagca cggcgggcct cgcggaggcg
      721 cgcgggcaga cgcccgagct cggagtcggg cagtcgcgca agggcggtcc acgcgcggtc
      781 gagagtctcg acgatgccgc ggcgctcctc cgcgccctgc gacaggaatt ccctctcgaa
      841 ggtctcgccg aattcgaggt acgacctctc ggtttccgag agctcttcgc gtccgatgat
      901 cgattccagg gatcgcgcgc gtgcggcgcg agcgcaggcg gcgtagagct gcctcgcgag
      961 ggccgggtgg tcttcgtgcg ttcgacccgc cccgatgccg ccggacatga gacgcgagag
     1021 cgaggggaga acgtccacgg gcggatagat gccccggcgg tcgagggatc gctggagcac
     1081 gatctgtccc tcggtgatgt acccggtcag gtccgggatc ggatgcgtga cgtcgcccga
     1141 cggaagagtg acgatcggga gctgggtgag cgagccggga cggccgcgca cgcggcccgc
     1201 gcgctcgaag atcgaggcga gatcgctgta gaggtaaccg gggtagccct tccgcccggg
     1261 cacttctccc gcggacgacg cgacctcgcg cagggcctcg ccgtaattcg tcacgtcgtt
     1321 caggatgacg agcacgtgaa aaccgcgatc gaacgcgagg tactctgccg ctgtcagcgc
     1381 gcaccgggga gtgatcagcc tctccggcgc gggatcgtcg gcgaggttga agaaggcgac
     1441 gacgcgggag agagcgccgc tcgcccggaa gtcctcgaga tagcggaacg cggtctcccg
     1501 cggcaacccg agcgccgcga agacgatggc gaattcgccg accccgggcg cgcgagcctg
     1561 acggacgatc tgcatcgcga gcgcgtcgtg gggaagcccg gattccgtga agatcggcag
     1621 cttctgcccg aggacgagcg cgttcaggcc gtcgatcgcg gacacgccgg tctcgagaaa
     1681 ctcgccgggg acgtcgcggc gcaccgggtt gatcggcgaa ccctcgatcg acctttgctc
     1741 gaccgcggcc ggagggggca ggccgtcgcg cgggcgtccc gtgccgtcga agaccctgcc
     1801 gacgagctcc gggccgacgc cgaaccgcag cggctcgccg cgaaatcgga cgcggcaggc
     1861 gtcgagggca agcccgtcgc tgcccgagaa gatctcgagc accgcggtgt cctccgagat
     1921 cccgacgacg cgcccgacgc ggatcgcgcc gtccggcccg atcacctcga cgtgctcccg
     1981 gtacgacgct ccggcgatgc ccgtgacgaa tccgagcggt ccccgcaggg cggcgagtcc
     2041 ccggtactcg agctccgtcc cgcggctcat cccgcctccg cgcgcgcctc gggcgaggag
     2101 agggccacgg ggagcgacga gaacgccgcc tcgagttcgg tctcgaggtc cgcgagccgc
     2161 ggcagctcgt cgtcgccgat ctcttccttg gccctctcga tgcgcgagag gacgggcatc
     2221 gcctcgatct cccgggaggt cgcgcctcgc tcgattgctt cgagcccgag atcccgtacc
     2281 cgcagcagga gccggagcaa tcgcgcctgg cgcgcgggcg agcaggaggc gtctttcggc
     2341 tcgaacgcgt tctgcgcgag gtatccctcc tggaagagcg atcccatccg cagggcgaag
     2401 cgctcgcgct cgggcaggct ctcggtgccg accagccgcg ccatccgctc gagctgcgcg
     2461 gctttttcga gcagggcgat cgcctcgcgg cgcagacgtc cccactcggg caccgtctcg
     2521 gcctcccacc acgaccgcac cgcaggcgac gcgtcccccg aggattcctc gagaccgatc
     2581 gccggaaaga tccgcgcctc cgcgcggcgc ggatcgaggc tccagaagca gcgagtgaaa
     2641 cggcgggtat gccgggtcac gggctccgtc agatcgccgc cgggaggcga tatcgcggaa
     2701 acgatcgaga ccgacccttc cgctgccgag agcgtccgca cccgtccggc gcgttcgtag
     2761 aacgcggcca gtcgcgtggc caggtaggcc gggtaaccct cctccgccgg catctcttcg
     2821 agccggcccg agatctcgcg gagcgcctcc gcccatcgag acgtcgaatc cgccagcacc
     2881 gccacgtggt aacccatgtc gcggtagtac tcggcgatcg tgaccgccgt gtagagggag
     2941 gcttcgcgcg ccgcgaccgg catgttggac gtgttcgcga gaaggacggt ccgctccgcg
     3001 agcgtccacc cggtccgcgg atcggcgagc tccgggagct cgtgcagcac ctgagccatc
     3061 tcgttgccgc gctcgccgca gccgacgaac acgatcacgt ccgcccgcat ccacttgcag
     3121 agctgttgct gcgtgatcgt cttgcccgtg ccgaatcctc ccggcatgcc ggcagcgccc
     3181 ccgcggggga ggggaaagaa cgtgtcgaga acgcgttggc ccgtcaagag cggaatcgag
     3241 agcggaagcg cttcggcgaa ggggcggggc gcgcgcacct tccatctctg gaagagcgga
     3301 agcgtgacgg tgccggtcgc ggtctcgatc ctcgcgacgg agtccgctac gcgtcgcgat
     3361 cctgtcggcg cgatctcgag gagacgtccc gcgatgccgg gcggtacgag gacgcgatgt
     3421 tcgatcgccc gcgtctccgg gacgactccg aggagcgacc ccgcgacgac ggatgcgccc
     3481 ggtgccacgc gaggctcgaa gccccacgcg cgatcccgat cgagcgccgg cagagaacgg
     3541 ccgcgcccga gaaagtcgcc ctcagccgcc gcgagcgcgg cgaggggccg cgcgaggccg
     3601 tcgagaaccg agccgagaag gcccggcccg agctccaccg tcaacggcga tccggtcgcg
     3661 aagaacgggt cgcccgcggc gaggcccgcc gtctcctcgt agacctgcgc ggtcgcgcgg
     3721 ttcccctcga gggcgatgac ctcgccggcg aggcggtcct ctccgacccg gacgacttct
     3781 cccatcgaca cgggagaggt cgtctccgcg cggaccaccg acccggagat cccgacgatg
     3841 cgtccgcagg gccgatcccc cgtctcgtct tctctcacgg gccgtagatc tccgtcaggg
     3901 ccgctctcca cagaggctcg aatcgccgcg cgcgagcctc gagactgttg tcgaatcgga
     3961 ccttcccgcc caccgtcgag acgacgcatc cgctttgcgg cagatctccg cgggcgcggg
     4021 cgaccgtccg gcgggcgcgt cgcgcgagtt cttcgacgaa ggcgtcgtcc gcgatggaag
     4081 cgtcggaggc ggacaccgag atctccactg cgcccgccgg gagcctctcg gccgcctcca
     4141 cggcaagagc gaggaggagt tcgcgtcgag aagcggcttc tcggtcgccg tcgagccgct
     4201 cgcgcgcgag ccgaatcgcc cgctcggtcc agcgctcgcg ctcctccagg gccgcgcggc
     4261 ggtcctccca gtcttcgcgg gcaagtcgag ccgagacctc ccggcgcgcc gccgcgagcc
     4321 gggccggacg atcgggaggc gtcacgtcgc cgctctgcgc ttccgaacgc agacggacga
     4381 tctcctcgtc ggtgtcccgc aggagccgct cgacctcgcg ggacgcctcc tcgcgaacgg
     4441 catcggccac ggacgcgggg ctcccgagag tcttcacggt gaggtgtccc cgcgcgccct
     4501 cgccgggagg accacgatcg caggaatgcc gccgcgaagt ctcaaccgtt cgagcgcggg
     4561 ggaaccgaga tcctcgacgg ccgcagagac gaagacgagg ccgatgcctt tctccgcttc
     4621 gatcgcggca aacgcccggt ccgcctcgcg ccggtcgcgc gggatcatgc cctcgattcc
     4681 cgcgagcgaa aagccgagcg cgtcgtcggc atcaccgacg aggcggatcc tcacggcagg
     4741 cgtccgagga tcatgatcgc gacgatcagg ccgtagatcg cgatcccttc tgcgagccct
     4801 acgaaaatga gcgcgcgccc ggccgtctcg ggcttctccg cgagcgctcc caccgcggcg
     4861 cttccgacga ccgcgacggc gatcgccgct ccgatcgctg cgaggcccgt gacgagggcg
     4921 gccgcgatga acccccactt ggcgagctct gtcgaggccg gggcggcgga cgccggcacc
     4981 tgggccgcga agagcggcag cacgagaagc ggcgacaggg ccgcggcgag agcgatcgtg
     5041 atccttcgct tcatggagac tctcctttgg cgaggcgcgg gcgaagcatg agcggccggt
     5101 acggttcgcc ccctccccgg aaaaacttgc cgaagaactc gtagtactcg aggcgcagga
     5161 cctggaccga gacggttagt ccttcgagga agatcatgac gatgttgccg gcgacgagca
     5221 gcaggatcga gagcgcgccc ccgccccgca accgcgagag cgtgtccgcg agagcgaaga
     5281 gggcgagaaa cgcgccggcg tggaccatcg cgaacgccgc gacccgcagg aacgagatcg
     5341 tgttcgcgaa ataggagacg acggcgtcga cgagctcgac cgaaccttcg agagccgcga
     5401 agaagaaggg actcttctcg acgccggcgc ggcgcggagc cggatggccc ttctcgagcc
     5461 agcggacgat cggcctgcgg agcagaatga gcgcgagtgg gatcgcgagc agggcgaaga
     5521 tcgccgcggc cggaaggtgg actttgcccc ccacgaacgc cctcgccgcg agcgcggcga
     5581 atacccagta cccgaaggcg ccgagcagtc cccgcggccc gaagacggcg agcgatcgct
     5641 ccccggagcg ccaggcgttc acggcgttca gaacgaggcc tccgctgacg aggacgacac
     5701 cgagagcgat cgcgagctcg agaaacgtgg tcaagtcccg gagcggatcc atccaca
//
LOCUS       DASWAV010000620         1802 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U2665
            k141_755048, whole genome shotgun sequence.
ACCESSION   DASWAV010000620 DASWAV010000000
VERSION     DASWAV010000620.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960316
            Sequence Read Archive: SRR10260007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 1802)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1802)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:47:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,929
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1802
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_755048"
                     /isolate="SMAG_U2665"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.36444 N 132.31889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1563)
                     /locus_tag="VF958_12860"
     CDS             complement(<1..1563)
                     /locus_tag="VF958_12860"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HEX9150046.1"
                     /translation="MPVTGTLIEGKYEILAKIKEGGMGTIYKVRHRLLDEVRVVKVMR
                     PQIGADAELSRRFTQEAKTATRLKHPNIGAILDFALDSDGMAYIVMEYIDGVNLTELL
                     AASGPPGLPLTLEIAHQTLLALGFLHRKNVVHRDVAPDNLMLTRNEDGTPQIKLIDLG
                     IAKPLDKTIDLTSTGVFLGKLKYSSPEQLGGLEEGETLDARSDLYSFGVVLYELLTGH
                     LPFPGESPRDLFAAHLFKPPIPFAVTDPEGRVPESVRAAVLKAIDKERRRRFFSAEDF
                     DREVIRLKHDLGLAQDPDATHRILAKVRGSRDVDKVSVTPSAQDRLDRHFLAQGTPAP
                     SSSQVTSALPQSGTSGEWEETVAAPLVETTGRRRPRMSLVLFGFGGAAALIAAALVIS
                     RSSSSSTNGEPPRASLPVPTAIVPAVVAPPTEVAQVPIVPTETAGAPEEPTSPPEALP
                     VEATERVRRAADDARNAPRAARSAAERARAPEAASALYESARRKEKDGQKSLGAGRYE
                     TALASFESATALF"
BASE COUNT          260 a          585 c          619 g          338 t
ORIGIN      
        1 aaacagcgcc gtcgccgatt cgaaggatgc gagcgccgtc tcgtaccggc ctgcccccag
       61 gctcttctgt ccgtcctttt ccttgcgccg cgcggattcg tagagcgccg aggcggcctc
      121 tggcgccctc gcgcgctcgg cggcgctccg cgcggctcgc ggcgcgttcc gcgcgtcatc
      181 cgcggcgcgc cggactctct ccgtcgcttc gacgggcagc gcctccggcg ggctcgtcgg
      241 ttcttccggc gcgcccgccg tctccgtcgg aacgatcggg acctgcgcga cctccgtggg
      301 gggcgcgacc accgcgggca cgattgccgt cggaacgggc aacgatgctc gcggcggctc
      361 tccgttcgtc gaagaagacg agctgcggct gatcacgaga gcggcggcga tcagggcggc
      421 ggctccgccg aatccgaaca ggacgaggga cattcggggt cgtcgtcgtc ccgtcgtctc
      481 gacgagcggt gcggccaccg tctcctccca ctctcccgat gtgccgctct gcggaagagc
      541 cgacgtgacc tgtgatgatg agggcgcggg tgttccctgg gcgaggaagt gacgatcgag
      601 cctgtcctgc gcgctcggcg tcacggacac cttgtcgaca tcccgcgaac cgcggacctt
      661 ggcgaggatc cggtgcgtcg cgtcgggatc ctgcgcaagc ccgaggtcgt gcttcaagcg
      721 aatgacttcg cggtcgaagt cctctgccga gaaaaacctc cgccgccgct ccttgtcgat
      781 cgccttgagg accgcggccc gaaccgactc gggtacgcgg ccttcgggat cggtgacggc
      841 gaagggaatc ggtggcttga agaggtgcgc ggcgaagagg tcgcgcggcg actctccggg
      901 aaaaggcagg tgtccggtca ggagctcgta gaggaccact ccgaacgagt acaggtcgct
      961 tctcgcgtcg agcgtctcgc cctcttcgag gccgccgagc tgctcggggg acgagtactt
     1021 caactttccg aggaagactc ccgtcgaggt cagatcgatc gtcttgtcga gtggcttcgc
     1081 gattccgaga tcgatgagtt tgatctgggg cgtgccgtcc tcgttgcggg tcagcatgag
     1141 gttgtcgggg gcgacgtcgc ggtgcacgac gttcttgcgg tggagaaacc cgagagcgag
     1201 gagcgtctgg tgggcgatct cgagcgtcag gggcaagccg ggcggcccgg acgccgcgag
     1261 gagctccgtc aggttgaccc cgtcgatgta ttccatgacg atgtacgcca tcccgtcgga
     1321 gtcgagagca aagtcgagga tggccccgat gttcggatgc ttgagacggg tcgcggtctt
     1381 cgcctcctgc gtgaaccgcc gcgagagctc cgcgtccgcg ccgatctgcg ggcgcatgac
     1441 cttgacgacg cggacctcgt cgagcaggcg gtggcggacc ttgtagatgg tccccatccc
     1501 gccttccttg attttcgcga ggatttcgta cttgccttcg atgagagttc cggtgaccgg
     1561 caagacacct cctgggcccg cgccgcgatt gtaaccgccg gagacgagcg tcagccttca
     1621 gatcgaggat tcgcgttctc ctttacaatg cgtcgatggc actaacgagt cctcgagagc
     1681 ctccggcact cgcgtccctt cccggcgcgc ggcagttctc gtcgcttctt ctctcggccc
     1741 gcccggccca atgggtgaag aacctggttg tgccgttgcc gtttctgttc ggcggagtgc
     1801 tg
//
LOCUS       DASWAV010000621         2308 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U2665
            k141_197929, whole genome shotgun sequence.
ACCESSION   DASWAV010000621 DASWAV010000000
VERSION     DASWAV010000621.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960316
            Sequence Read Archive: SRR10260007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2308)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2308)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:47:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,929
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2308
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_197929"
                     /isolate="SMAG_U2665"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.36444 N 132.31889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            59..1516
                     /gene="gatA"
                     /locus_tag="VF958_12865"
     CDS             59..1516
                     /gene="gatA"
                     /locus_tag="VF958_12865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020378333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HEX9150047.1"
                     /translation="MTRPAGVVETAADVASRRRSAEETLTEALRRVETLDGRVGAFLE
                     VTPQQALAEAQAADARVAAGEDLPLAGVPMAIKDNMWVAGRQATCASRILQGFRPPTD
                     STAVARLRQAGAVFVGRSNMDEFAMGSSTENSALQLTRNPWDLERIPGGSSGGSAAAV
                     ASGMVPGALGSDTGGSIRQPAACCGVVGFKPTYGRVSRYGLVAFASSLDQIGPLCRDV
                     ADAARVYRVLAGPDPLDSTSTREDPGNPEAATGKGVRGMRVGFLAEAEAEGLDADVAK
                     NLEDARRAFTSAGAHVTSVSVARAPHAIAIYYVVASAEASSNLARYDSVRYGPRPASD
                     DVASLYVESRTRGFGPEVKRRILLGTFTLAAGYYEAYYGRAMRARAVLTEDFRKAFEE
                     VDVIVCPSIPSPAFRIGEKTEDPLTMYLSDIFTVPASLAGLPAISVPSGFSRGGLPLG
                     LQVLGPRFGEENVFAAAGGLERELALPEEMPRGIR"
     gene            1513..>2308
                     /locus_tag="VF958_12870"
     CDS             1513..>2308
                     /locus_tag="VF958_12870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9150048.1"
                     /translation="MKALAAAAALFFVGLGAPAEFRGEKTIVVSPELHVSIGEGDEIT
                     LSARPLPGEGLDAFIKRFTEDPKTKKEILSQNEGVKLLQKDVFVRVPYRLLSENYRRI
                     AIEALFPEDTSDAQGWNHRVRALTGKPESFWRIAEWFTGDGANYREIRTANQAESLET
                     ESGQTVRIPLKLLSPTFRDAAEAAAGAPVPLEFGRDEKGRYAAYRLQRGEALYSAVVV
                     RLTGRVHAEDVNAKAAEIAQRSGITDVRSIPVGYAVKIPVEDLSPEF"
BASE COUNT          350 a          801 c          802 g          355 t
ORIGIN      
        1 acgctccggc gctctcacac ggattcgggg tggtcccgcg cgtggtcgga ggaggggagt
       61 gacccgccct gccggcgtgg tggagacggc ggcggacgtt gcctcccggc gtcggtccgc
      121 cgaggagacc ctgaccgaag cgctccgacg ggtcgaaacc ctcgacgggc gcgtcggagc
      181 gttcctcgaa gtcacgccgc agcaggcact cgccgaggcg caagcggccg acgcgcgcgt
      241 cgccgccggt gaggaccttc cgctcgccgg cgtcccgatg gcgatcaagg acaacatgtg
      301 ggtggccggt cgccaggcga cgtgcgcctc ccgaatcctc caggggttcc gcccgccgac
      361 ggactcgaca gcagtcgcgc ggcttcggca ggcgggcgcc gtcttcgtcg ggcgctccaa
      421 catggacgag ttcgcgatgg gctcctcaac cgagaacagc gcgcttcagc tgacgcgcaa
      481 tccctgggac ctggagcgca tcccgggcgg gtcgtccggc gggagcgcgg cggcggtggc
      541 atccggcatg gtgccaggcg ctctcggctc cgatacgggc gggtcgatcc gccagccggc
      601 ggcctgctgc ggcgtcgtcg gcttcaagcc gacgtacgga cgggtgtccc gctacggcct
      661 cgttgcgttc gcctcgtcgc tcgaccagat cggtccgctc tgtcgcgacg tcgccgacgc
      721 ggcgcgggtc taccgggttc tcgccgggcc cgacccgctc gactcgacct cgacgcgcga
      781 ggacccgggg aatccggagg cggccaccgg gaaaggcgtc cgcggaatgc gggtgggctt
      841 cctcgcggag gcggaggcgg aggggctcga cgctgacgtc gcgaagaacc tcgaggacgc
      901 gcggcgggcc ttcacgtccg ccggcgcgca cgtgacctcg gtctcggtcg cgcgcgcgcc
      961 gcacgcgatc gcgatctact acgtcgttgc gagcgccgag gcgtcctcca acctcgcccg
     1021 ttacgactcg gtccgatacg gaccgcgccc cgcgtcggac gacgtggcgt cgctgtacgt
     1081 cgagagccgc acgcgcggct tcggaccgga ggtcaagcgc cgcatcctcc tcggcacttt
     1141 taccctggca gccggatatt acgaggctta ctacgggcga gcgatgcgcg ctcgcgcggt
     1201 gctcaccgaa gacttccgca aggctttcga ggaagtcgac gtgatcgtct gcccgtcgat
     1261 cccgtcgccc gcgtttcgca tcggcgagaa gaccgaggat ccgctcacga tgtacctgtc
     1321 cgacatcttt accgtcccgg cctcgctcgc ggggctgccg gcgatctccg ttccctccgg
     1381 gttttcccgg ggcgggcttc ccctcggcct ccaggtcctc ggaccgcgct tcggtgaaga
     1441 aaacgtgttc gctgcggccg gcggactcga acgggagctc gcgctgccgg aggagatgcc
     1501 ccgaggaatc cggtgaaagc tctcgccgcc gcggccgctc tctttttcgt cgggctcggc
     1561 gctccggcgg agttccgcgg tgagaagacc atcgtcgtct cgcccgagct ccacgtctcc
     1621 atcggcgagg gagacgagat cacgctctcc gcccgcccgc tgcccggcga agggctggac
     1681 gccttcatca agcgattcac ggaggacccg aaaacgaaga aggagatcct ttcgcagaac
     1741 gaaggagtga agcttctgca aaaggacgtg ttcgttcgcg tgccctaccg gctgctctcc
     1801 gagaactacc gacggatcgc gatcgaggcc ctcttccccg aggacacgag cgacgcgcag
     1861 gggtggaatc atcgcgttcg cgcgttgacg gggaagccag agagcttctg gcggattgcc
     1921 gagtggttca ccggcgacgg agcgaactac cgtgagatcc gcacggccaa ccaggcggaa
     1981 tcgctcgaga cggaaagcgg acagacggtc cggattccgc tgaagctcct ctcgccgacg
     2041 ttccgcgacg cggcggaagc ggccgcggga gcgcccgtcc cgctcgaatt cggtcgcgac
     2101 gagaaaggcc gctacgccgc ctatcggctc cagcgcggcg aagcactcta ctccgccgtg
     2161 gtcgtgcgcc tgaccggtcg tgttcacgcc gaggacgtca acgccaaggc cgcggagatc
     2221 gcccagagga gcggaatcac cgacgtgcgc tcgatcccgg tcgggtatgc cgtcaagata
     2281 ccggtcgaag atctgtcgcc cgagttcc
//
LOCUS       DASWAV010000622         7684 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U2665
            k141_517858, whole genome shotgun sequence.
ACCESSION   DASWAV010000622 DASWAV010000000
VERSION     DASWAV010000622.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960316
            Sequence Read Archive: SRR10260007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 7684)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7684)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:47:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,929
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7684
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_517858"
                     /isolate="SMAG_U2665"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.36444 N 132.31889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2959)
                     /locus_tag="VF958_12875"
     CDS             complement(<1..2959)
                     /locus_tag="VF958_12875"
                     /inference="COORDINATES: protein motif:HMM:NF013069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HEX9150049.1"
                     /translation="MIARVIDFSLRNRFLIIGFALLLVAGGIAAVRTNPVDAIPDISE
                     TQIIVFADWPGRSPQEVEDQVTYPMVVNLQGLAGVKAVRSSSAFGFSMANVIFEDGTD
                     LYFARTRILERLNLIGSLLPKGVRPVLGPDATAVGQIFWYTVEGAGQDLGALRAIQDW
                     YVRYQLNSVPGVAEVASIGGYVRQYQVDVDPNRLAAYGLSIRDVITAVEMSNNNVGGK
                     VVEQGGLEYVVRGIGLIRDVADVEKTVISSRGGTPIYVSNVGTVQMGPEFRRGALEKN
                     GSEVVGGVVTMRYGESAPEVIARIKEKIADLSKGLPPGVRIVPFYDRSSLIERAVGTL
                     KRALLEEIALVTLAHVVFLMHFRSILIVTLPLPLAILTSFILMKVFGITSNIMSLGGI
                     AIAIGVLVDAGIVITENIFRHMESESGRAPLVEIVRKASKQIGRPIFFSMVIILLAFL
                     PVFALTGEEGKLFHPLAFTKTFAMIGATLLSVTLVPVLATFLVRGRLHREEDNPVMRV
                     TRRLYEPLLAAALRRKKTTLVIAVALFLASLVLVAGAGVMLFPLKPLLILARVPRAVD
                     RIEAVQAKLDGAIAPGIGREFMPPLDEGTLMFMPVTSNAVSLSQAVEIMKKQDAALRS
                     FPEVASVVGKVGRVESPLDPAPINMYETLVELKPRDEWRPGMTKEKLMAEMSDRSRLP
                     GVTAIWQQPIRNRIDMLATGIPTQVGVKIFGPDLAVLEKKANEIAEVIRSVPGAADVY
                     AEQILGSPYLEIEVNREAAARYGVHVGDVLDVIETAIGGENLTTTVEGRNRFPVRVRY
                     SRELRDDPDSIRRILVPAAGSGGMLPASAPPAERMEETTTETSGARARFAGAPSPLGA
                     RVQIPLSEVAEIRFRPGPSMISSENGLLRERVFLNVRGRDVGSLVDEAKRVVESKVAL
                     PAGYFVEWAGQFEHQLRARNRLAVVVPVCLAIIFFLLYLTYHSIKEAAHVILAIPFAL
                     TGGNFLLWLLHRLA"
     gene            complement(2956..4326)
                     /locus_tag="VF958_12880"
     CDS             complement(2956..4326)
                     /locus_tag="VF958_12880"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HEX9150050.1"
                     /translation="MTEERKRRTGRALAILAVALVLGVSGAVLLTRRPKPPGTPAEGA
                     TPTPLSVGAPVGEIAGRKVLYWYDPMAPGSRFDKPGKSPFMDMDLVPKYADEEPAAAT
                     TAPAAPPVALSAAAIRASGVATTAVVRQPLSLSIRAVGQIAADETKLERVAARLAGRV
                     EHLYANFTGQPVRRGAPLFSLYSPDLVATEREYLLTLENRRRLRGASADAGRSAESLV
                     EAARDRLRLWGISNEQIRSLEESGRPELAVTFRSPVSGVVLQKTAVEGQYVQEGTELY
                     LLGDLSSVWLVAQVYESDIGRIRVGQPVVSTLTAYPGREFRGTVVFIEPVLDPETRTA
                     RVRAVLPNALGELKPGMFADARFEASLGNVLSIPKSAAIDTGARQIVYVEASPGNFSP
                     REVRLGATAGDRREVLKGLGEGEKVVSAANFFVDSQSQLAAGSSIQWSGALEVKTTPT
                     PQGGRP"
     gene            complement(4319..5617)
                     /locus_tag="VF958_12885"
     CDS             complement(4319..5617)
                     /locus_tag="VF958_12885"
                     /inference="COORDINATES: protein motif:HMM:NF014385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HEX9150051.1"
                     /translation="MKRVRSCRIVPLAFLGVAAQLLVAAAATSEAAVPRLRAAVERAI
                     AGNPEISEMELRIEAAKQRVPQSLALPDARLVAGAINVPVPDVSFAREDMTMKMVSLE
                     QMIPASGKRRAAAGIATAEVEMAEAMHVAHVNRLVADVADAYYEVAELDARLTIAQRS
                     LERLGRVSESVRTRYRVGQGGLPDVLLAGVEETKVRDRIRALEADRASAAARLNTLQG
                     LAPAEPVAPIAFSAADQTIPDRAALSAALERSPDVLQAQAEVRRAERDLEGARLERRP
                     DLTFMASYGERQRRDDMIGATVGINLPFFQRRRLAARIAEKEAELGAAKKRLESVRLR
                     LSREVEDAGIAIGREVDRAALYRGTILSQDRTAAEAAEQAYAVGRIDFQTYVRAVLAV
                     DDDEAETVMRETALPRARAKLLASAGVPLPEISGLEVRHD"
     gene            6261..7523
                     /locus_tag="VF958_12890"
     CDS             6261..7523
                     /locus_tag="VF958_12890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9150052.1"
                     /translation="MPHPHAPADMADMPASEGHDLFQSDMALMTGMTPHDEMAGMAMP
                     GWQLMVLGVGRLGYNNQGGRSGATVFESSNWNMVHAAHDLGGGRLSLMMMNSLEPWTF
                     EKRGSPELFQTGEAYRGRPLVDRQHPHDFFMNLSATYRFPFGKDAAAWFQLAPVGEPA
                     IGPTAFMHRASSGENPTAPLGHHWQDSTHIAFNVITAGGGSKWFAVEGSAFHGAEPDE
                     KRTDVEGGTIDSAAGRLKIFLPGGWSAQVSHAYIHEPEELSPGNTRRTTASLHYGAAG
                     DRPLAATLLWGRNDEEHGTTDSWLLEGAWQITPEDHAYGRLEWVEKDRHLLQFKGAEV
                     HPAVALPEVAEITALTIGYLRDFVSRPALRVGAGADVTGYRFPSGLRRVYGDQPVSWH
                     LFLRARWGKPHGAHSGSHEKSTGLAGSD"
BASE COUNT         1318 a         2571 c         2577 g         1218 t
ORIGIN      
        1 cggcgaggcg gtggagcaac cagaggagga aatttccgcc cgtcagcgcg aagggtattg
       61 ccaggatgac gtgcgcggcc tctttgatcg agtggtaggt cagatagagc aggaaaaaga
      121 tgatcgccag acataccggc acgacgaccg cgagccggtt gcgcgcgcgg agctggtgct
      181 cgaattggcc cgcccattcg acgaagtatc cggccggaag ggcgaccttc gactcgacga
      241 cccgtttcgc ctcgtccacg agcgatccga cgtcacggcc ccgaacgttc aggaataccc
      301 gctcccgcag gagcccgttc tccgacgaga tcatcgatgg ccccgggcga aaccggatct
      361 ctgcgacctc gctgagcggg atctgcacgc gtgcgccgag agggcttggg gcgccagcga
      421 accgcgcccg ggctccggag gtttccgtcg tggtctcttc catgcgctcc gccggcggcg
      481 cggaggccgg taacatccct cccgaccccg ccgcgggaac gagaattcga cggatcgaat
      541 cgggatcgtc tcggagttca cggctgtacc gcacccggac gggaaaccgg ttccttccct
      601 ccacggtcgt ggtgaggttc tctccgccga tggccgtctc gatgacgtcg agaacgtcgc
      661 cgacgtgcac gccgtagcgg gcggccgcct cccggttgac ttcaatttcg aggtagggcg
      721 atccgaggat ttgctccgca tacacgtcgg ctgcgccggg tacggagcgg atcacctccg
      781 caatttcgtt cgccttcttc tcgagtacgg cgagatcggg tccgaagatc ttgacgccga
      841 cctgcgtggg gatcccggtc gccagcatgt cgatccggtt gcggatcggc tgctgccaga
      901 tcgcagtcac ccccggcagc cgggaccgat cgctcatctc cgccatgagc ttctctttcg
      961 tcatccccgg gcgccactcg tcgcgaggct tcaactcgac gagcgtctcg tacatgttga
     1021 tcggcgccgg atcgagcggg ctttcgacac ggccgacctt gcccactacg gaagcgacct
     1081 ccgggaacga ccgcaacgcg gcgtcctgct ttttcatgat ctcgacggcc tggctgagcg
     1141 acacggcatt cgacgtcacc ggcatgaaca tcaacgttcc ttcgtcgagc ggcggcatga
     1201 actcccgccc gatacccggc gcgatggctc cgtcgagctt cgcctgcacc gcttcgatcc
     1261 ggtcgaccgc gcgcgggaca cgggcgagga tcaacagcgg tttcagcggg aagagcatga
     1321 cgccggctcc cgcgacgaga acgaggctgg cgagaaagag ggcgacggcg atgacgagag
     1381 tcgtcttctt gcgccgcaga gcggcggcga ggagcggctc gtagaggcgc cgcgtcacgc
     1441 gcatgaccgg gttgtcttct tcgcggtgga gccggccgcg aacgagaaag gtcgcgagga
     1501 ccggcacgag agtgaccgaa aggagagtcg ccccgatcat cgcaaacgtc ttcgtgaagg
     1561 cgaggggatg gaacagcttc ccctcttcgc cggtcagcgc gaacaccggg aggaacgcga
     1621 ggaggatgat caccatcgag aagaagatcg gtcttccgat ctgcttcgaa gccttacgga
     1681 cgatctcgac gaggggagcc cggccgctct cgctctccat gtggcgaaag atgttctcgg
     1741 tgatcacgat gcccgcgtcc accagtactc cgatcgcgat ggcgatccca ccgagcgaca
     1801 tgatgttcga cgtgattcca aacaccttca tcaggatgaa agaagtgagg atcgcgagcg
     1861 ggagggggag cgtcacgatc aggatcgagc ggaagtgcat caggaacacg acgtgcgcca
     1921 gcgtgacgag cgcgatttcc tcgagaagcg ccctcttgag cgttccgacg gctcgctcga
     1981 tcaggctcga acggtcgtag aacggaacga tccgaacgcc cggcggcagt cccttcgaga
     2041 ggtcggcgat cttttccttg atacgggcga tgacctccgg cgcgctctcc ccgtacctca
     2101 tcgtgaccac gccgcccacg acctccgagc cgttcttttc gagcgccccg cgccggaact
     2161 ccggccccat ctggaccgtt ccgacgttgg agacgtagat cggcgtgccg ccgcgcgagg
     2221 agatgaccgt cttctcgacg tcggcgacgt cccggatcaa gccgatgccg cgaaccacgt
     2281 attcgaggcc gccttgctcc accacctttc cgccgacgtt gttgttcgac atctcgaccg
     2341 ccgtgatcac gtctcgaatc gagagtccgt atgcagcgag acggttcggg tcgacgtcga
     2401 cctggtactg gcgaacgtag ccgccgatcg atgcgacctc ggcgacaccc gggacggagt
     2461 tcaactggta gcggacgtac cagtcctgaa tcgcgcggag cgctccgaga tcctggcccg
     2521 ctccctcgac cgtgtaccag aagatctggc cgacggcggt ggcgtcgggc ccgagcacgg
     2581 gccgaacgcc cttcggcagc agcgacccga tcaggttcag gcgctcgagg atccgcgtgc
     2641 gcgcgaagta gaggtccgtg ccgtcttcga agatcacgtt cgccatcgag aagccgaacg
     2701 cggaggagga tcggacggcc ttgacgccgg cgagtccctg gaggttgacc accatggggt
     2761 aggtgacctg gtcctcgacc tcctgcggcg accgccccgg ccagtcggcg aaaacgatga
     2821 tctgcgtctc ggagatgtcg ggaatcgcat cgacgggatt cgttctgacc gcggcgatgc
     2881 caccggccac gagaagaagc gcgaacccga tgatcaggaa ccggttcctg agagagaaat
     2941 cgatgacgcg cgcgatcacg gcctgcctcc ctgcggagtc ggcgtggtct tgacctcgag
     3001 ggccccgctc cactgaatcg agctcccggc ggcgagctgc gactgcgagt cgacgaagaa
     3061 gtttgcggcg gaaacgacct tctccccctc gcccagtcct ttgaggacct cgcggcgatc
     3121 gcccgcggtt gcgccgagtc ggacctcgcg tgggctgaaa ttcccggggg aggcttcgac
     3181 gtagacgatc tgccgcgcgc cggtatcgat ggcggcggac ttcgggatcg agagaacgtt
     3241 cccgagcgat gcctcgaacc gggcgtcagc gaacatgccg ggcttcagct ccccgagggc
     3301 gttcggcagc acggcgcgta cgcgcgcggt gcgggtctcg ggatcgagaa ccggctcgat
     3361 gaagacgact gtcccgcgga attcgcgccc cggataggcg gtgagtgtcg agacgaccgg
     3421 ctgaccgacg cgaattcgac cgatatcgga ctcgtacacc tgcgcgacga gccagacgct
     3481 cgagaggtcc cccaggagat agagctcggt cccctcctgg acgtactggc cttcgacggc
     3541 ggtcttctgc aggaccaccc cagagaccgg cgagcgaaag gtcaccgcga gctcgggccg
     3601 cccgctctct tcgagagatc ggatctgctc gttcgaaatt ccccacagcc ggagccggtc
     3661 gcgcgccgct tccacgagag actcagccga gcggcctgcg tcggccgatg cgccccgaag
     3721 gcgccggcga ttttcgagtg tcaggaggta ctcacgctcc gtcgcgacga ggtccgggct
     3781 gtagagcgag aagagcggcg cgccacgccg gaccggctga ccggtgaagt tcgcatagag
     3841 atgctcgacc cgcccggcca gccgcgcggc gacgcgctcg agcttcgtct cgtccgccgc
     3901 aatctgaccc accgcccgga tcgacaagga aagcggttgg cggacgaccg ccgtcgtcgc
     3961 aacgccggag gctcggatcg ccgcggccga aagcgcgacc gggggcgccg ccggggccgt
     4021 cgttgcagcc gccggctcct cgtccgcgta cttcggaacc aggtccatgt ccatgaaagg
     4081 cgacttgccc ggtttgtcga accgggagcc gggcgccatc gggtcgtacc agtagaggac
     4141 cttgcgcccc gcgatttcgc cgaccggagc accgaccgag aggggagtgg gcgtcgcgcc
     4201 ttcggccggc gtcccggggg gcttcgggcg ccgcgtgagc agcaccgcac cgctgacgcc
     4261 gaggaccaga gccacggcaa ggattgcgag ggcccgcccc gtccgacgct ttctctcctc
     4321 agtcatggcg cacctccagg cccgagatct cgggcagggg cactccggcg gacgcgagga
     4381 gcttcgcccg tgcgcggggc agcgccgtct cgcgcatcac ggtctccgcc tcgtcgtcgt
     4441 cgacggcgag gacggctcga acgtacgtct ggaagtcgat ccggccgact gcgtaggcct
     4501 gttcggcggc ctccgcggcg gtccggtcct ggctcagtat ggtgccgcga tagagcgccg
     4561 cccggtcgac ttcgcgaccg atcgcgattc ccgcatcctc gacctcgcgg gagagcctga
     4621 ggcggaccga ctcgagccgc ttcttcgcgg cgccgagctc cgcttccttt tcggcgatgc
     4681 gcgcggcgag gcgacggcgc tggaaaaagg gcaggttgat tccgaccgtc gcgccgatca
     4741 tgtcgtcccg tcgctgccgc tctccgtacg aagccatgaa cgtgaggtcg ggacgtcgtt
     4801 cgaggcgcgc gccttccagg tcgcgctcgg cgcggcgcac ttccgcctgc gcctgcagaa
     4861 cgtcggggct gcgttccagc gcggcgctca gggcggcgcg gtccggaatc gtctgatccg
     4921 ccgcggagaa cgcgatcggt gccaccggtt cagccggggc gagcccctgg agcgtattca
     4981 accgcgccgc ggcggaagcg cggtcggcct cgagcgcgcg gatccggtcg cgaaccttcg
     5041 tctcttcgac tcccgcgagg aggacgtcgg gaagcccccc ctggccgacc cggtagcgcg
     5101 tgcgaacgga ctcggaaacc cgaccgagtc gttcgaggct gcgttgtgcg atcgtgagcc
     5161 gagcgtccag ctccgcgacc tcgtaatagg catcggcgac gtcggcgacc aggcggttga
     5221 cgtgcgcgac gtgcatcgcc tcggccatct cgacttcggc cgtcgcgatc ccggccgcgg
     5281 cgcgccgctt accggaagcg gggatcatct gctcgaggct caccatcttc atcgtcatgt
     5341 cctcgcgcgc gaacgacacg tcgggcaccg ggacgttgat cgcaccggcg acgaggcggg
     5401 catcgggcag cgcaagcgac tgggggaccc tctgtttcgc tgcttcgatc cggagctcca
     5461 tctccgagat ttcggggttc cccgcaatgg ctcgttcgac ggcggccctc aggcggggca
     5521 cggccgcttc gctcgtcgcc gcagcggcaa ccaggagttg cgcggcgacg ccaaggaacg
     5581 cgagcggcac gattctgcag gaacggactc gtttcatgac gacactccat tgcttcgcaa
     5641 cggctgcaaa agcgcagccg acgtcactca gaaacgtgcg tctctagagg cgaaggatgg
     5701 aggaaggagg gccgaaggcg gtcggaggcg acggggatgc gaacgggtcg agcgatggcg
     5761 acacggccga aaccagcagg gcggaggtca aagagttcaa cggaaggcac ggaggatcga
     5821 gcgaagcgcg ttcgacggtg accgcgggag aggaaggttt cgcgctcgcc accttcgggg
     5881 cgcagccctc ctcgcagcag ggcatcgcgg cacctattcc ctggccgctg tcggctttcg
     5941 aacagcaaga agaacagagg gcgccgatcg gtgccgcgag gaaggcgaac gcgaggacga
     6001 cgatgagcaa cttccgcacg atagccattg tcaccccaaa cgggccccaa cggggatgca
     6061 aatgaatcag gttcccgaca ggaatttttc gggctgcgca gtctcttgtg ccgggcgtgg
     6121 tgcgacttcc gaagccgggc cgggcgaacg ccctgcgcct tctcaacgcg attctgttcg
     6181 ccggctcgac ttcgaccgcc gcgcttgcgc aacacgaaca ctctgcgcca cccgcaccaa
     6241 ctcccacccc ttcgaccgcc gtgccgcacc cgcatgcgcc ggccgacatg gccgacatgc
     6301 ccgcgtccga gggccacgac ctcttccagt ccgacatggc tctgatgacc gggatgacgc
     6361 cgcacgacga gatggccggc atggcgatgc cgggatggca gctcatggtc ctcggagtgg
     6421 ggcgactcgg ctacaacaat caggggggac ggtcgggcgc gactgtgttc gagtccagca
     6481 actggaacat ggtccacgcc gctcacgatc tcggaggagg gcggctctcc ctcatgatga
     6541 tgaactcgct cgagccctgg accttcgaga agcggggctc gccggaactc ttccagaccg
     6601 gcgaggcgta ccgcgggcgg cccctggtcg accgacagca tccccacgac ttcttcatga
     6661 atctctcggc gacgtaccgg ttcccgttcg gaaaggacgc agccgcatgg ttccagctcg
     6721 cgcccgtggg cgagccggcg atcggtccga ccgcgttcat gcaccgcgcc tcctccgggg
     6781 agaatccgac ggcgcccctc gggcaccact ggcaggattc gacgcacatc gcgttcaacg
     6841 tcatcacggc ggggggcggc tcgaaatggt tcgccgtcga aggctccgcg ttccatggcg
     6901 ccgagccgga cgagaagcgc accgacgtcg agggcggcac gatcgactcg gccgcgggtc
     6961 gcttgaagat ctttctgcct ggaggttggt cggcgcaggt ctctcatgcg tacatccacg
     7021 agcccgaaga gctctcgccg ggcaacaccc gccgcacgac cgcatcgctc cattacggag
     7081 cagcggggga ccgtccgctc gccgcgacac ttctctgggg aagaaacgac gaagagcacg
     7141 gcacaaccga ctcgtggctc ctcgaaggag cctggcagat cactccggag gatcacgcct
     7201 acgggcggct cgagtgggtc gagaaggacc ggcacctcct tcagttcaaa ggagccgaag
     7261 tccaccccgc agtggctctt ccggaggtcg ccgagatcac cgcgctgacg atcggctacc
     7321 tgcgcgactt cgtctcacgg cccgccctcc gcgtcggagc gggggcggac gtcacaggct
     7381 accgcttccc gtcaggtctc cggcgcgtct atggagacca gccggtgtcg tggcacctgt
     7441 tcctgcgcgc ccgctggggc aagccgcacg gggctcactc cgggtcacac gagaagtcga
     7501 ccgggctcgc cggctcggat tgacggccgc ccgagccgtc agcgctcttc gagggttcgg
     7561 agggccgccc cgagccttgt ccgggcctct tcgggcagac cggcgaggtc ggtttgcgtc
     7621 cgggcgtcgg cgacctcgaa catcttctga ggatccagga tggaaactcg gatggcgccg
     7681 cctt
//
LOCUS       DASWAV010000623         2615 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U2665
            k141_592002, whole genome shotgun sequence.
ACCESSION   DASWAV010000623 DASWAV010000000
VERSION     DASWAV010000623.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960316
            Sequence Read Archive: SRR10260007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2615)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2615)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:47:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,929
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2615
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_592002"
                     /isolate="SMAG_U2665"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.36444 N 132.31889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..218)
                     /locus_tag="VF958_12895"
     CDS             complement(<1..218)
                     /locus_tag="VF958_12895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011237864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="KUP/HAK/KT family potassium transporter"
                     /protein_id="HEX9150053.1"
                     /translation="MVYGDIGTSPLYAFRVCFAGRHAVPLEPMNVLGVLSLIFWTLVL
                     VVGVKYHGWILRLHNRGEGGILALMELVR"
     gene            complement(323..2155)
                     /locus_tag="VF958_12900"
     CDS             complement(323..2155)
                     /locus_tag="VF958_12900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014400435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium transporter Kup"
                     /protein_id="HEX9150054.1"
                     /translation="MCLGALGVVYGDIGTSPLYAVRECLHGPHGVPPTPGNIFGVLSL
                     IFWSLIIVVTLKYHVYVLHLDNRGEGGILALMGLIRPDRQKTAAMRWILVALGVFGAA
                     LLYGDGMITPAISVLSAVEGLSIATSVFDRFVVPITISILFGLFFFQKHGTGRVGAVF
                     GPVMLVWFSTLAVLGVSSIVQEPSVLGAINPSHAVRFFAHNGFHGFLVLGLVFLVATG
                     GEALYADLGHFGERPIQVDWFFLVGPSLLLNYFGQGALLLRNPGAADNPFYRLAPGFL
                     LYPLVVLSTAATIIASQAVISGAFSLTRQAVQLGYMPRVQIDHTSVKEIGQIYIPSVN
                     WALAAATVALVLGFRSSSNLAAAYGVAVTTTMVITTFLAYFVSRDRWGRPLALIVTIG
                     FLTADLAFFVANIVKVPHGGWFPLLVAIGVFTIMTTWRSGRRILTERIRESALSDELF
                     VRDVERRSPPRVPGTAVFMDRTVDGIPLALLHNLKHNKVLHEKVVLLTVDTKEIPYVP
                     DEEEMEVRDLGKGIYRIILRYGFMESPDVPDALRRLEHPGLRFDESTSFFLGRETLLA
                     TKRPGMAIWRERLFSWMTRNAQAAPLYFRLPPNRVVEVGAQIEL"
     gene            complement(2241..>2615)
                     /locus_tag="VF958_12905"
     CDS             complement(2241..>2615)
                     /locus_tag="VF958_12905"
                     /inference="COORDINATES: protein motif:HMM:NF024206.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HEX9150055.1"
                     /translation="DGFTPLGLAAFFGHPEAARLLLAAGSDPDQPARNATRVAPIHSA
                     VAGGTVEIVRDLLVCGADVHARQDAGFTALHGAAAEGSVEMVRLLLAHGADRFAKNDA
                     GKTPADLARERGREQAAELLLR"
BASE COUNT          531 a          812 c          891 g          381 t
ORIGIN      
        1 cggacgagct ccatcaacgc gaggattccc ccctcgccgc ggttgtggag acggaggatc
       61 cagccgtgat acttgacccc cacgacgaga acgagcgtcc agaagatgag agagagcacg
      121 ccgaggacgt tcatgggctc gagcgggacg gcgtgccggc ccgcgaagca gacgcgaaag
      181 gcatagaggg gactcgtccc gatgtcgccg tagacgatcg cgagcgcggc gacggagagg
      241 aggaagagct cccgtccccg tggctcgcgg ggataccgcg ccgtgccgct ctcgaaggca
      301 ggaggacctt cgacgacctc gctcacagct cgatctgcgc gccgacttcc acgacgcggt
      361 tcggcggcaa ccggaagtag agcggcgccg cctgcgcgtt gcgggtcatc caggagaaga
      421 gccgctcgcg ccagatcgcc atgcccggcc gctttgttgc cagcagcgtc tcgcgaccga
      481 gaaaaaacga agtcgactcg tcgaaccgga gacccgggtg ttcgagccgc cgcagggcat
      541 ccggcacgtc cgggctctcc atgaaaccgt atcgcaggat gatccggtaa atgcctttgc
      601 ccagatcccg aacctccatc tcttcctcgt cggggacgta agggatctcc ttcgtatcca
      661 ccgtcagcag gacgaccttc tcgtgcagaa ccttgttgtg cttcaggttg tggaggagag
      721 cgagcgggat tccgtcgacg gtgcggtcca tgaacacggc ggtgcccgga actcgggggg
      781 gcgaccggcg ctcgacgtcg cgcacgaaca gctcgtcgga gagcgcgctt tcacggatcc
      841 gttccgtcag gatcctgcgc ccgctgcgcc acgtcgtcat gatcgtgaag acgccgatcg
      901 ccacgagcag cggaaaccat ccgccgtgcg ggaccttgac gatgttcgcc acgaagaaag
      961 ccaggtcagc ggtcagaaac ccgatcgtga cgatgagagc gagcggcctc ccccagcggt
     1021 cacgtgagac aaagtacgcg aggaaagtcg tgatgaccat tgtcgtcgtc actgcaacgc
     1081 cgtaagcggc ggcgaggttc gaggaagagc gaaatccgag gacgagcgcc accgtcgctg
     1141 cggcgagcgc ccagttcacg ctcgggatat agatctgccc gatctccttg acagaggtgt
     1201 gatcgatctg gacgcgcggc atgtaaccga gctgcaccgc ctgtcgcgtc agcgagaaag
     1261 cgccggaaat gaccgcctgc gaagcgatga tcgtggccgc tgtcgagaga acgacgagcg
     1321 gatagagaag aaaccccgga gcgagtcgat aaaacgggtt gtcggcggcg cccggattcc
     1381 gcaggaggag agctccctgg ccgaaataat tcagaaggag ggagggtccc accaggaaga
     1441 accagtcgac ctggatcggg cgctccccga aatgaccgag gtctgcgtag agggcctcgc
     1501 cccccgtcgc cacgaggaaa accaggccga gaactagaaa tccgtgaaag ccgttgtgag
     1561 cgaagaaacg cacggcgtgt gacggattga tcgcgccgag gaccgaaggc tcctggacga
     1621 tcgaggagac gccgagcacc gccagagttg aaaaccagac cagcatgacg ggcccgaaga
     1681 ctgctccgac gcggcccgtg ccgtgtttct ggaagaagaa gagaccgaag agaatcgaga
     1741 tcgtgatcgg cacgacgaag cggtcgaaga cggaggtggc aatgctcaag ccttcgaccg
     1801 cggaaagtac ggagatcgcg ggcgtgatca tcccgtctcc gtagaggagt gccgccccga
     1861 acacgccgag cgccacgagg atccagcgca tcgccgccgt cttctgacga tccggccgga
     1921 tgagccccat cagagcgagg atccccccct ccccgcgatt gtccaggtgc agcacgtaga
     1981 cgtggtactt gagcgttacg acgatgatga gcgaccagaa gatcagcgag aggacgccga
     2041 agatgttgcc cggggtcggc ggcactccgt gtggcccatg caggcactct cggacggcgt
     2101 agagagggct cgttccgatg tcgccgtaga cgacgccgag agcgcccagg cacatgaggg
     2161 cgaggtactt gccgcgagga ggcgtcgcgt gcggatttgt cgggagcgcc tcggcctggg
     2221 gtgcctccac gaaggggaat ctaacgcaga aggagctcgg ccgcctgctc ccttcccctc
     2281 tctcgcgcga gatccgcggg ggtcttgccg gcgtcgtttt ttgcgaagcg gtccgcgccg
     2341 tgggcgagca gtaatcgcac catctcgacg ctcccttccg cggcggctcc gtggagagcg
     2401 gtgaaccccg cgtcctggcg ggcgtgaacg tcggcgccgc acacgagcag gtcccgcacg
     2461 atttcaacgg ttccccccgc gaccgccgag tgaatcggcg cgacgcgggt cgcgttgcgc
     2521 gcgggctgat cggggtcgct gccggcggca aggagaagcc gtgcggcctc cggatgcccg
     2581 aagaacgccg cgaggccgag cggcgtgaaa ccgtc
//
LOCUS       DASWAV010000624         6428 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U2665
            k141_675384, whole genome shotgun sequence.
ACCESSION   DASWAV010000624 DASWAV010000000
VERSION     DASWAV010000624.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960316
            Sequence Read Archive: SRR10260007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 6428)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6428)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:47:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,929
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6428
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_675384"
                     /isolate="SMAG_U2665"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.36444 N 132.31889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..483)
                     /gene="selD"
                     /locus_tag="VF958_12910"
     CDS             complement(<1..483)
                     /gene="selD"
                     /locus_tag="VF958_12910"
                     /EC_number="2.7.9.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015617929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004756 - selenide, water dikinase
                     activity [Evidence IEA];
                     GO_process: GO:0001887 - selenium compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_except=(pos:complement(424..426),aa:Sec)
                     /transl_table=11
                     /product="selenide, water dikinase SelD"
                     /protein_id="HEX9150056.1"
                     /translation="MTYRKAGHPALSTFASSAGUACKIGQFDLSALLAKLPAPADPNL
                     LVGNASADDAAVYRIGEDRVMVQTIDFFTPVVDDPYLFGQIAAANALSDVYAMRARPV
                     LALALVAFPTTVLPAEVLEDILRGGADKVTEAGAVVGGGHSIDHDVPLYGLAVTGMAR
                     E"
     gene            complement(509..739)
                     /locus_tag="VF958_12915"
     CDS             complement(509..739)
                     /locus_tag="VF958_12915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9150057.1"
                     /translation="MFRVSAIERALPDDLRRTVSDHPILAITAGALVGFYLGRSHGRQ
                     ILSALVGVGLNAGASTARRMLGIAPQPRRAAR"
     gene            complement(767..1039)
                     /locus_tag="VF958_12920"
     CDS             complement(767..1039)
                     /locus_tag="VF958_12920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9150058.1"
                     /translation="MAKETPFDRARDYVEEKIDQTKDVLGDSFDGVSDEVRKQVKATA
                     KRIEKDYGYIWDDVRGYVKNNPGTAVALSVGIGFLLGYLLHGGDDD"
     gene            1162..2544
                     /locus_tag="VF958_12925"
     CDS             1162..2544
                     /locus_tag="VF958_12925"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015350393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEX9150059.1"
                     /translation="MATLRFLGACGTVTGSKFLLEAGSRRVLFDCGVFQGPPDLKQKN
                     WSPPDFDPGSVDAVVLTHAHIDHTGYLPRLGKLGYGGPVYVTPPTAGLLEYLLPDAAH
                     LHEEDARYANRKGYSSHRPALPFFDSDEARRALGLLRPVAFHERAELAPGVAFTFRRV
                     GHILGAAFIEVEADGRRLVFSGDVGQRGVPILKDPETPDSADYLLLESTYGDRLHPAG
                     SPREELGRVVAEVVARRGVLLIPAFAVGRAQDVLYHLRSLQREGAIPADLPIYVDSPM
                     AVSAVDLYCAALSEHDLEMAKLQSEGDCPISGPSVHLVRDRIESKALNERRGPMIIIS
                     ASGMLSGGRILHHLKRRLPDPETTLLFVGFQAAGTLGRRILEGAREVRIHGETVTVRA
                     EIRDVPALSAHADQRGLVEWSGALAAAPRAVYLVHGEPKGREALAAALRSKPGWNVVL
                     PEEGQAVELQ"
     gene            2564..3838
                     /locus_tag="VF958_12930"
     CDS             2564..3838
                     /locus_tag="VF958_12930"
                     /inference="COORDINATES: protein motif:HMM:TIGR00765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YihY family inner membrane protein"
                     /protein_id="HEX9150060.1"
                     /translation="MDEARDSNVEPAGRVTMVVERAKSRAPFVPEPLLRAVVTFREIV
                     VDTFRGFTADRGADLAGSLAFTTLLTAVPLLATFSLFLAAFFKEKNDQVLDLVNRILP
                     YHTARLTENLRDFVAESTAISGIGLVALIVASVRLIFIVEATFNAVWGAPKRRAWFQR
                     IAIHSFALFSLALLVGAIGSGVQILRKFSLLETVLVSPLAEKFFPFAAEFAVLTLLYH
                     YLPNAYVRWTSAALAGGTIAILLELLRTVFGKYVAMLSRMNLITGSLSFLMFALISVF
                     LVWVLILLGVELTHVLQTKIGKSRRRGRVPAGRAENAIRMLLRLADGGIHRFRDLYHD
                     QEASSVEAEKILECLRDAKIVEGDAARGFALAEPPDKVTVARVVDAISPNLYTITPEE
                     DDRVVQMLEPLFARLDSERRALLGATLSDLKS"
     gene            complement(3835..5013)
                     /locus_tag="VF958_12935"
     CDS             complement(3835..5013)
                     /locus_tag="VF958_12935"
                     /inference="COORDINATES: protein motif:HMM:NF013188.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HEX9150061.1"
                     /translation="MEHGELGSFLLAFAAALFGGKLLGELCERIRQPAVLGELLAGVL
                     LGPSLLGLVPLSAGILLLAEIGVILLLFEVGLETDLGELLSVGGPAMTVALVGMVLPF
                     LGGYVLTRVAGFSALTAIFVGAALTATSIGITSRVLSELDMLSSREGQIILGAAVADD
                     ILGLVVLAVVSQIAATGSVSMWQAGKAAILSFVFLLVALVIGMPLGRVLIRVVKRASV
                     RGILVAASVAFALLAALGAQTAGSAAIVGAFAAGLVLARTDRSSEIHTAVRPIVDVFA
                     PVFFVSIGAQVNLQYLNPTVSENRQALLLALGLIVVGVLGKFAAGFASWGRVRRTFVG
                     AGMIPRGEVGLIFAQIGKQNGALPEPVFIAVVIAVFATTFVTPPLLKALRPRTPIPGT
                     "
     gene            5085..5161
                     /locus_tag="VF958_12940"
     tRNA            5085..5161
                     /locus_tag="VF958_12940"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5119..5121,aa:Ala,seq:cgc)
     gene            5327..6022
                     /locus_tag="VF958_12945"
     CDS             5327..6022
                     /locus_tag="VF958_12945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017720404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HEX9150062.1"
                     /translation="MIRIRRANERGHFDHGWLNTYHTFSFADYHDPAHMGFRSLRVIN
                     EDRVAPGQGFGAHRHRDMEIVSYVLEGALAHQDSMGNSGVIRPGDVQRMSAGTGVQHA
                     EFNGSKTEPVHFLQIWILPERRDIPPSYEQKTFANEVKRGHLRLIGSPDGREGSVSIQ
                     QDVELYASVLDAGGEVRHALRSGRAAWLQVARGELELNGAPLSSGDGAAITDERELVI
                     AGNGSELLLFDLG"
     gene            complement(6035..>6428)
                     /locus_tag="VF958_12950"
     CDS             complement(6035..>6428)
                     /locus_tag="VF958_12950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9150063.1"
                     /translation="HFHLGMVAVLRSRHGEALAEFEKALVLSHSQPLEVPIRAYGLAV
                     GGRRTEALAILGKVDGGEKGGTRTPSVPVANASVYAFLGLRDEAFRCLEVGFADRFPE
                     MVFLKVHPLLSELRGDPRFADLVRRMGL"
BASE COUNT         1074 a         2249 c         2090 g         1015 t
ORIGIN      
        1 ctcccgcgcc atccccgtga ccgcgaggcc gtagagcggc acgtcgtgat cgatcgaatg
       61 gccgccgccc acgaccgcgc cggcctccgt caccttgtcg gccccccctc tgaggatgtc
      121 ctcgagcact tcggccggga gaacggtcgt gggaaaggca acgagggcga gcgccagcac
      181 gggccgcgcg cgcatggcgt acacgtcgga gagcgcgttc gccgccgcga tctggccaaa
      241 gaggtagggg tcgtcgacca cgggcgtgaa gaagtcgatc gtctggacca tcacccgatc
      301 ctccccgatg cggtacaccg ccgcgtcgtc ggcggacgcg ttgccgacca gcagattcgg
      361 atccgccgga gccggcagct tcgcgagaag cgcggagagg tcgaactgac cgatcttgca
      421 ggctcagccc gcgctcgacg cgaacgtcga gagcgcaggg tgacccgctt tgcggtacgt
      481 cacgaccgga ttatagggag atagcagcct agcgcgccgc ccgtcgcggc tgcggcgcta
      541 ttccgagcat ccggcgcgcg gtcgaggcac cggcattgag tcccacgccc accagagccg
      601 aaaggatctg gcgcccgtgg ctccgcccga gatagaagcc cacgagcgct cccgccgtga
      661 tcgccaggat cggatgatca gacaccgtcc ggcgcaggtc gtccggaagc gccctttcga
      721 tcgcggagac gcgaaacatc gctaccgccg gatgcgggga gaccgtctag tcgtcgtccc
      781 cgccgtgaag caggtaaccg agtagaaagc cgatcccgac cgagagggcg accgccgttc
      841 cggggttgtt cttcacgtag ccgcggacgt cgtcccagat gtagccgtag tccttttcga
      901 tccgcttcgc cgtcgccttg acctgcttgc ggacttcgtc gctcacgccg tcgaacgaat
      961 cgccgaggac gtccttcgtc tggtcgatct tctcctcgac gtaatcacgc gctcgatcga
     1021 acggggtttc cttcgccatt cttcacctcc gcgtcaacac cggatcttat acctgcgaac
     1081 atcgtgccgc gtccggtcgg gacccgccga ggccgtcggc ccgaccttcc cggcgaaacc
     1141 gcgggcataa tccggtccac gatggcgact ctgcggttcc tcggcgcctg cgggacggta
     1201 accggatcga agtttctcct ggaggcgggt tcgcggcggg ttcttttcga ctgcggcgtt
     1261 tttcagggcc ctccggatct gaaacagaag aactggtcgc cgccggactt cgatcccggt
     1321 tccgtcgacg cggtggtcct gacgcacgcc cacatcgacc acacgggata tctgccgcgg
     1381 ctcggcaaac tcggctacgg cggccccgtg tacgtcacgc caccgaccgc ggggctgctc
     1441 gaatacctgc tgcccgacgc cgcgcacctg cacgaggaag acgcccggta cgcgaaccgg
     1501 aagggctact cgagccaccg gccggccctg ccgttcttcg attcggatga ggcgcggcgc
     1561 gccctcggcc tgctccgccc ggtcgctttc cacgaacgtg ccgagctcgc gccgggggtc
     1621 gccttcacgt ttcggcgcgt gggacacatt ctcggggcag cgttcatcga ggtggaggcg
     1681 gacggcagac gcctcgtttt ctccggcgac gtcggacagc gcggcgtgcc gatcctgaag
     1741 gaccccgaga cgcccgactc cgcggactat ctcctgctcg aatcgaccta cggggatcgg
     1801 ctgcatcccg ccgggtcgcc acgcgaagag ctcggtcgcg tcgtcgccga ggtcgtcgcg
     1861 agacgcggcg tcctcctcat tcccgcgttc gcggtcggac gcgcacagga cgtgctctac
     1921 cacctccgct ccctgcagcg cgaaggcgcg atccccgccg atctcccgat ctacgtagac
     1981 agtccaatgg cggtctccgc cgtggacctc tactgcgccg cgctctccga gcacgacctc
     2041 gagatggcga agctccagag cgagggggac tgtccgatct cggggccttc cgttcacctc
     2101 gtccgggatc ggatcgagtc gaaggcgctg aatgaacgac gtggcccgat gatcatcatt
     2161 tccgcctccg ggatgctctc gggcggccgc atcctccacc atctgaagcg ccggctgccc
     2221 gatcccgaga cgactcttct tttcgtcggc ttccaggcgg cgggcactct cggccggcgc
     2281 atcctcgagg gcgcacgcga ggtccgcatc cacggagaaa ccgtaaccgt gcgcgcggag
     2341 atccgggacg tcccggccct ctcggcgcac gcggatcaac gcggcctggt cgagtggtcg
     2401 ggcgcgctcg cggcagcgcc gcgggcggtc tatctggtgc acggcgagcc gaaggggcgg
     2461 gaagccctcg ccgcggcgct gcgctcgaaa ccgggctgga acgtcgttct ccccgaggaa
     2521 gggcaggcgg tcgagctaca gtgaaacgca aggcgtaaac gtggtggacg aagcccgaga
     2581 ttcgaacgtc gagccggccg ggcgcgtcac catggtcgtg gagcgcgcga aaagtcgcgc
     2641 gccgttcgtc cccgagccgc ttctgcgggc ggtcgtcacg tttcgcgaga tcgtcgtcga
     2701 tacgttccgg ggcttcaccg cggaccgcgg cgcggacctc gcgggctcgc tcgccttcac
     2761 gaccctcctg accgccgtcc ccctgctggc gaccttctcg ctcttcctcg ccgccttctt
     2821 caaggagaag aacgaccagg tcctggacct cgtgaaccgg atcctgccgt accacaccgc
     2881 ccggctgacc gagaacctcc gcgacttcgt cgcggagtcg accgcgatct ccgggatcgg
     2941 cctcgtcgcc ttgatcgtcg cgtcggtgcg gctcatcttc atcgtcgagg cgacgttcaa
     3001 cgccgtctgg ggcgccccga aacggcgcgc ctggttccag cgaatcgcga tccacagttt
     3061 cgcgctcttc tcgctcgctc tcctcgttgg cgcgatcggg tccggagtcc agatcctgcg
     3121 gaagttttcg ctgctcgaga cggtgctcgt ctcgccgttg gccgagaagt tcttcccgtt
     3181 cgcggcggag ttcgcggtgc tgacgctcct ctaccactac cttccgaacg cgtacgtccg
     3241 ctggacctcg gcagcgctcg cgggcgggac catcgccatc ctcctcgagc tcctgcggac
     3301 cgtcttcggg aaatacgtcg cgatgctttc gcgcatgaac ctgatcaccg gttcactgtc
     3361 cttcctcatg tttgcgctga tctccgtctt cctcgtctgg gtcctcatcc ttctcggggt
     3421 cgagctgacc cacgtcctgc agacgaagat cgggaagagt cggcgacgtg ggcgcgtgcc
     3481 ggcagggcgc gcagagaacg cgatccggat gctcctccgg ctcgcggacg gaggcattca
     3541 ccgcttccgc gacctctacc acgaccagga agcctcgtcg gtggaggcgg agaagatcct
     3601 cgagtgtctc cgcgacgcga agatcgtgga gggcgacgcc gcccgcggat tcgcgctcgc
     3661 ggaacccccg gacaaggtca cggtggcgcg cgtcgtcgac gcgatctcgc cgaacctcta
     3721 cacgatcaca cccgaagaag acgatcgcgt ggtccagatg ctcgagcctc tcttcgcccg
     3781 cctcgattcc gaacgccgcg ccctcctcgg ggcgacgttg agcgacctga agagctaggt
     3841 cccggggatc ggcgtccgcg gccgcagcgc cttcaagagc ggcggagtga cgaacgtcgt
     3901 cgcgaagacc gcgatcacga ccgcgatgaa cacgggctcc ggcagcgccc cgttctgctt
     3961 tccgatctgg gcgaagatca gaccgacctc tccgcgcgga atcatgccgg ctccgacgaa
     4021 ggtcctccgg acgcggcccc aggaagcgaa accggcggcg aacttgccga ggacgccgac
     4081 gacgatcaga ccgagagcga gcagcagagc ctggcggttc tccgacaccg tcggattcag
     4141 atactgcagg ttgacctggg ctccgatcga aacgaagaag acgggcgcga agacgtcgac
     4201 gatgggccgc accgccgtgt ggatttcgct gctccgatcg gtccgggcga gcacgaggcc
     4261 cgcggcgaac gcgccgacga tcgccgccga gccggctgtc tgcgctccga gggcggcgag
     4321 cagggcgaag gcgaccgatg cggcgacgag aattccgcgg acgctcgccc gcttgacgac
     4381 gcggatgaga acgcggccga gcggcattcc gatgacgagg gcgacgagga gaaagacgaa
     4441 ggagaggatc gccgccttcc cggcctgcca catcgagacc gagcccgtcg ccgcgatctg
     4501 gctgacgacc gcgagaacca cgaggccgag aatgtcgtcg gcgaccgcgg cgccgagaat
     4561 gatctgtccc tcgcgcgaag agagcatgtc gagctccgag aggacacggc tcgtgatccc
     4621 gatcgaggtc gccgtgagcg ccgctcccac gaagatcgcg gtgagcgccg aaaaccccgc
     4681 gacccgcgtg aggacgtagc cgcccaggaa tggcagaacc atgccgacca gggcaaccgt
     4741 catcgccggc cccccgacgc tgaggagctc gcccagatcg gtctcgaggc cgacctcgaa
     4801 gagaagcagg atcacgccga tttccgccag cagcaggatt ccggcggaga gcggcacgag
     4861 accgagaagc gagggaccga gcagaactcc cgcgaggagc tcccccagga ccgcgggctg
     4921 gcggatccgt tcgcaaagct cccccagaag ctttccgccg aagagcgccg ccgcgaacgc
     4981 gaggagaaag gaaccgagct cgccgtgttc cactgcagca gatggtaaac gacagctttc
     5041 ccgggggcgg cccgcgtgct atcctgcgcc ccctcttcga gcgtggggct gtagctcagt
     5101 ttgggagagc gcctgaatcg cactcaggag gtcgtcggtt cgatcccgat cagctccacc
     5161 aagcacgtcg gcttcgcttg ctcggctccg cctcgtggcg cttcgcctcc cgcggcgcga
     5221 gcctcctccc tcggtcacct cgggagactt ctcgcgccgc gagctcaacc agcgcacgtc
     5281 caggatggga gatcctcgcg ccgcgaaact ctgatctact cggccgatga tccgcatccg
     5341 gcgcgcgaat gagcgcgggc acttcgacca cggctggctg aacacgtacc acacgttctc
     5401 gtttgccgac tatcacgatc ccgcccacat gggctttcgg tcgctgcgag tcatcaacga
     5461 ggatcgggtg gcccccggac agggcttcgg cgcccaccgc caccgtgaca tggagatcgt
     5521 ctcgtacgtg ctcgaagggg cgctcgcgca ccaggacagc atgggcaact ccggggtgat
     5581 tcggcccggc gacgtccaga gaatgagcgc cggcacgggc gtccagcacg ccgagttcaa
     5641 cggatcgaag accgagccgg tgcacttcct gcagatctgg atccttccgg aacgccgcga
     5701 cattcctccg agctacgaac agaagacgtt cgccaacgag gtgaagcgcg gccaccttcg
     5761 cctcatcggc tcgccggacg ggcgcgaagg ctccgtctcg atccagcagg acgtcgagct
     5821 ctacgcgagc gtgctcgacg ccggtggaga agtccgccac gcgctccgga gcggacgcgc
     5881 ggcgtggctg caggtcgctc gcggcgagct ggagctgaac ggtgccccgc tctcctcggg
     5941 cgacggcgcc gcgatcaccg acgagcgcga gctcgtgatt gcgggcaacg ggtcggagct
     6001 cctgctcttc gacctcggct gatccgccgc acagtcaaag tcccatccgc cgcacgaggt
     6061 cggcgaacct cggatctccg cgcagctcgg aaagcagcgg atggactttc aagaacacca
     6121 tctcggggaa gcggtcggca aaaccgacct cgaggcaacg aaaggcctcg tcgcggaggc
     6181 cgaggaaggc gtagaccgac gcgttggcca ccggaacgct cggcgtccgg gtgcccccct
     6241 tctcccctcc atcgactttg cccaggatcg cgagcgcttc cgtccgccgg ccgcccaccg
     6301 cgagcccgta cgcccgtatg gggacttcga gcggctgact gtgcgagagg acgagcgctt
     6361 tctcgaactc cgccagcgcc tccccgtgtc tcgatcgaag gactgcgacc atgccgagat
     6421 ggaagtgc
//
LOCUS       DASWAV010000625         1665 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U2665
            k141_236400, whole genome shotgun sequence.
ACCESSION   DASWAV010000625 DASWAV010000000
VERSION     DASWAV010000625.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960316
            Sequence Read Archive: SRR10260007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 1665)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1665)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:47:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,929
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1665
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_236400"
                     /isolate="SMAG_U2665"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.36444 N 132.31889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            85..714
                     /locus_tag="VF958_12955"
     CDS             85..714
                     /locus_tag="VF958_12955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite oxidase-like oxidoreductase"
                     /protein_id="HEX9150064.1"
                     /translation="MNIPTSDRLPPGQVLTEKWPVLTYAATPRVDLQTWTFRCFGLVE
                     NALSWTWNEFLALPRVEVTSDIHCVTRWSRFDNRWEGVAVSEILRRVGLRPEAVAVMA
                     HSGGYTTNLSLADLWDDDVLLAFRHDGRDLEPDHGGPCRLVVPKLYFWKSAKWVRAFE
                     FLDVNAPGFWEVNGYHLHADPWKEERYSGQETDAMQRMRAESARRLRGR"
     gene            complement(718..972)
                     /locus_tag="VF958_12960"
     CDS             complement(718..972)
                     /locus_tag="VF958_12960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxidase"
                     /protein_id="HEX9150065.1"
                     /translation="MTLDNDLVEAIRRDYRTAPIDERDRTMLDYVAELTRDATRITPE
                     HHERLRAVGFDDRGILQITLIASWFNYINRVADSLGVGRS"
     gene            complement(1003..1281)
                     /locus_tag="VF958_12965"
     CDS             complement(1003..1281)
                     /locus_tag="VF958_12965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9150066.1"
                     /translation="MTWIKTIPFSEAEGKLREAFERERALYPGEYATPTGVTDEAGSI
                     VASHSLIPDALYHAFATFGVLMSPDLPLTRRQHEMITTVVSVTNRCFY"
     gene            complement(1278..>1665)
                     /locus_tag="VF958_12970"
     CDS             complement(1278..>1665)
                     /locus_tag="VF958_12970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9150067.1"
                     /translation="ALAKLHSITNAPIRATALLAAMATLLILSGTFPQILAFFMCPTL
                     VLVALAASALFVVRRRGTNVPTFRSPGYPVTSALFVLLVAVVVLLIAFARPVAALAGF
                     AVVLAGLPVFAFAGRGTGDRRPGGSP"
BASE COUNT          298 a          524 c          587 g          256 t
ORIGIN      
        1 gctgcagatg aaagcgaaga tcggcgccgt ggaggcaccg atcgcgctgg tcgtcaaggg
       61 gcaggccccg cagtaacttt tcgtatgaac attccgacat ccgatcgcct gccgcccggc
      121 caggtgctca ccgagaagtg gccggtgctc acgtacgccg ccacgccgcg cgtcgacctg
      181 cagacctgga ccttccgatg cttcggtctg gtcgagaacg cgctttcgtg gacgtggaac
      241 gagttccttg ccctcccgag agtcgaagtg acctcggaca tccactgcgt gacccgctgg
      301 agccggttcg acaatcggtg ggagggggtc gcggtttcag aaatccttcg tcgcgtgggc
      361 ctccgaccgg aggcggtcgc cgtgatggct cacagcgggg ggtacacgac gaacctctcc
      421 ctggcggacc tttgggacga cgacgtcctc ctcgccttcc ggcacgacgg ccgggacctc
      481 gagcccgatc acggcggccc ctgccgcctc gtcgtgccga agctctactt ctggaagagc
      541 gccaagtggg tccgcgcgtt cgagttcctc gacgtcaacg cgccggggtt ctgggaggtc
      601 aacggatatc acctccacgc cgacccgtgg aaggaggagc gctactccgg ccaggagacc
      661 gacgcgatgc aacggatgcg ggcggagtcg gcgcgccgcc ttcgcggtcg gtgaaggtca
      721 gctccgaccc acgccgagcg agtcggcgac ccggttgatg tagttgaacc acgaagcgat
      781 gagcgtgatc tggaggatgc cgcggtcgtc gaatccgacg gcgcgaagcc gttcgtggtg
      841 ctccggggtg atccgggtcg cgtcgcgcgt caactcggcg acgtagtcga gcatcgtgcg
      901 gtcacgctcg tcgatgggcg cggtccggta gtcccgccgg atcgcttcca ccagatcgtt
      961 gtcgagggtc acccgacgca gaaactctgc gtgagattcg attcaataga agcagcggtt
     1021 ggtcaccgac acgaccgtcg tgatcatctc gtgctggcgg cgagtgagag gcaggtccgg
     1081 tgacatcaag actccgaacg tcgcgaacgc gtgatacaga gcatcgggga tcagagagtg
     1141 ggacgcgacg atgctgccgg cttcgtcggt gacaccggtc ggcgtcgcat actccccggg
     1201 gtagagcgcg cgctcgcgct cgaaggcctc gcggagcttc ccctccgcct cggagaacgg
     1261 gatggtcttg atccacgtca tggcgatcct cccggccgcc ggtcgcccgt ccctcgcccg
     1321 gcgaaggcga agacgggcag accggccagc acgaccgcga atcctgccag agcggcgacc
     1381 ggccgggcaa acgcgatgag cagaacgacc acggcgacga gcaggacgaa gagcgcggag
     1441 gtcaccggat agccgggcga tcgaaaggtg ggtacgttcg tcccgcgccg tcgcacgacg
     1501 aagagcgcgg acgcggcgag cgccacgagg acgagcgtcg ggcacatgaa gaacgcgagg
     1561 atctgcggga aggtcccgga gaggatgaga agcgtggcca tcgcggcgag aagggcggtc
     1621 gcccggatcg gagcgttcgt tatcgaatgg agcttcgcga gcgcc
//
LOCUS       DASWAV010000626         4741 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U2665
            k141_236412, whole genome shotgun sequence.
ACCESSION   DASWAV010000626 DASWAV010000000
VERSION     DASWAV010000626.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960316
            Sequence Read Archive: SRR10260007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 4741)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4741)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:47:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,929
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4741
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_236412"
                     /isolate="SMAG_U2665"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.36444 N 132.31889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..562
                     /locus_tag="VF958_12975"
     CDS             <1..562
                     /locus_tag="VF958_12975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007644050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF885 domain-containing protein"
                     /protein_id="HEX9150068.1"
                     /translation="IHEAVPGHHLQIALAQELTGLPQFRRHGFFTAFVEGWGLYAESL
                     GDEMGFYTDPYSKFGQLTYEMWRAVRLVVDTGMHAFKWDRQRAIDFMKANTAKTEHDI
                     TVEIDRYIVWPGQALAYKMGELEFKELRARAKKQLGERFDVRKFHDAVLLSGALPLDV
                     LDERIDAWIAREKAAPVKPVAPGPKS"
     gene            690..1274
                     /locus_tag="VF958_12980"
     CDS             690..1274
                     /locus_tag="VF958_12980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011877849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HEX9150069.1"
                     /translation="MSDSDFFSPVERGKAHVGFFVNGEPVEVAFAPHKTLLEVLREDL
                     GLTGTKHGCELGECGTCTVLVDGKPVLSCLSLGLECEGRRVETIEGMATAAGLHPLQK
                     AFADLGAAQCGYCTPGFLLTAKALLDVNPRPTLTEIKEALAGNLCRCTGYVKIFEAVE
                     LAAAWMRGEDAQPTKESLYGSDFDESGLLPVIRR"
     gene            1399..3831
                     /locus_tag="VF958_12985"
     CDS             1399..3831
                     /locus_tag="VF958_12985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019306824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin cofactor-binding domain-containing
                     protein"
                     /protein_id="HEX9150070.1"
                     /translation="MKRRTRVVGRPYPKVDAAAKVTGQTKFADDLFLPRMLHCKLLRS
                     KVAHAKLVRVDISRALAMPGVFAVATGQDLPIAFGILPVSQDEHALALDRVRYVGDPV
                     AAVAAQDEDAAFAALDAIEVEYEPLPPIGSIDDAVARPEPRIHEYGDSGNFHKLVSME
                     FGDTEKGFAAADRVYEDLFFYEGSTHLPLEQHAAVADFGPDGKLTLWSSTQTPHYVHR
                     ALAKVLEIPAPRIRVIATPNGGGFGGKSDPFNHEIVVAHLARVTGRPVKICLTREEVF
                     YCHRGRHPTLMRVKTGVKRDGAVTALHFQTVLDGGGYGSYGVASTYYTGALQTVTYRV
                     PSYRFEGARVFTNKPPCGPKRGHGTPQPRYALEVHLDKIACDLGLDPAEMRKSQLVPP
                     NSVTANFLRIGSMGLGACIDKVVAASGWRERRGKLPFGRGLGLACSSYICGAGLPIYW
                     NSMPQSGVQLKLDRSGGATVFCGSIDIGQGSDSVLAYVVGEVLGLDPFDIRVVTADTD
                     LTPVDLGSYSSRVTLMTGNAALQAAERARELLVDAVAAKLAIPKARVALAEGRVFDVE
                     EPGTGLTFADAVVLAESKFGTIGTVGSYTPPPSPGRYRGAGVGPSPAYSYSAAVVEVD
                     VDPETGIYTVPKVWIAHDVGKCINPVLVVGQVEGSVYMGLGEAMMEEMAYRSGVNANV
                     VHKFPSLLEYKSPTTKETSEVVTFLIEDPDPNGPFGAKEVGQGPLLPIPPAVSNALFD
                     AVGVRVDEIPATPEKVHAALKLGAAGQVARVGPVRFPEIPYPPPMRVLTPEEGGDGRA
                     VEAAVTGAGGVR"
     gene            3834..>4741
                     /locus_tag="VF958_12990"
     CDS             3834..>4741
                     /locus_tag="VF958_12990"
                     /inference="COORDINATES: protein motif:HMM:NF013134.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD binding domain-containing protein"
                     /protein_id="HEX9150071.1"
                     /translation="MMRLPRFRYLSPRTVAEAVAMRADAGPDGAYVAGGTDLYPNMKR
                     RQQTPRVVVGLSRVASLSRIRESDGRILLGAGLVLAEVENDSQVRAGYPGLAHAVSEI
                     STPPLRNMGTLGGNLMLDTRCNYYDQNYEWRKAIDFCMKKDGQICWVAPGSPKCLAVQ
                     SADTVPVLIAMGARAVLASPEGEREIAVEDLYRNDGIEYVTKRPGELLTEVVLPRENG
                     WRASYRKLRRRGSFDFPVLSVGAAVWRDGGVVTAARLVLGGVASAPVRLPASEAALVG
                     RPLDDASIADAAAAAAGPSRPMDNTD"
BASE COUNT          772 a         1584 c         1605 g          780 t
ORIGIN      
        1 gatccacgag gccgtccccg gccaccacct gcagatcgcg ctcgcgcagg agctgacggg
       61 gcttccccag ttccgccgcc acggattctt caccgcgttc gtcgagggat ggggactcta
      121 cgccgagagc ctcggcgacg agatgggctt ctacaccgac ccgtattcca agttcggtca
      181 gttgacctac gagatgtggc gggcggtccg actcgtcgtc gacacgggga tgcacgcgtt
      241 caagtgggat cggcagcgcg cgatcgactt catgaaggcg aacaccgcga agaccgagca
      301 cgacatcacc gtcgagatcg accggtacat cgtctggccc ggccaggcgc tcgcttacaa
      361 gatgggagag ctcgagttca aagagctgcg tgcgcgggcg aagaagcagc tcggcgagcg
      421 gttcgacgtg cggaagtttc acgacgccgt gctgctctcc ggagcgcttc cgctcgatgt
      481 cctggacgag cggatcgacg cctggatcgc ccgggagaaa gcggcgccgg tcaaacccgt
      541 ggccccgggt ccgaagtcct aagtcctgaa ggggaaggaa cttgccggtc ccgggaccga
      601 ggactcgaga cagagaactg gggactgacc agtcagtcag tgaatcggtt ctttctctct
      661 cgaaacaaga ggcttacact tcctcgaaaa tgtctgactc cgactttttt tcgccggtgg
      721 aacgggggaa ggcgcacgtc gggttcttcg tcaacggaga gcccgtcgag gtcgcgttcg
      781 cgccgcacaa gactctcctg gaggttctcc gcgaggacct cgggctcacc gggaccaaac
      841 atggctgcga gctcggcgag tgcgggacgt gcacggtcct cgtcgacggg aagccggtcc
      901 tctcgtgcct ctctctcggt ctcgagtgcg agggacggcg ggtcgagacg atcgagggaa
      961 tggcgacggc ggcgggcctc catccgctcc agaaggcgtt cgccgacctg ggcgcggcgc
     1021 agtgcggcta ctgcacgccg gggttcctgc tgacggcgaa ggcgctcctc gacgtgaatc
     1081 cgagaccgac gctcaccgaa atcaaggagg cgctcgccgg aaacctctgc cgctgcaccg
     1141 gctacgtgaa gatcttcgag gccgtcgagc tcgcggccgc gtggatgcgc ggagaagatg
     1201 cccagccgac gaaggaatcg ctttatggaa gtgacttcga cgaatcggga ctccttccgg
     1261 tgattcgtcg atgaacccgg gaagcaagct tccgtttgaa agttctcagc cgccgctcgc
     1321 ggagcgagcg ccgcggcgcc agtcaccgcc gcgcaccagt actgaattcg acgagtcggg
     1381 gctgcttccg gtcgtgaaat gaaacgtcgc actcgcgtgg tcgggcgccc gtatcccaag
     1441 gtcgacgcag ccgcgaaggt caccgggcag acgaagttcg ccgacgacct cttccttccg
     1501 cggatgctcc actgcaagct cctccgttcg aaagttgccc acgcgaagct cgtccgcgtc
     1561 gacatctccc gcgcgctggc gatgccggga gtttttgccg tcgccaccgg ccaggacctc
     1621 ccgatcgcgt tcggcatcct tccggtctcg caggacgagc atgcgctcgc gctcgaccgc
     1681 gtccgctacg tcggtgaccc ggtagcggcg gtcgccgcgc aggacgagga cgcggcgttc
     1741 gcggcgctcg acgcgatcga ggtcgaatac gagccgctgc cgccgatcgg ctcgatcgac
     1801 gacgccgtgg cgcgcccgga gccgcggatt cacgagtacg gcgactccgg caacttccac
     1861 aagctcgtct cgatggagtt cggcgacacg gagaaaggtt tcgccgccgc ggatcgagtg
     1921 tacgaagacc tctttttcta cgagggctcg acgcaccttc cgctcgagca gcacgcggcg
     1981 gtcgccgact tcgggcccga cgggaagctc acgctgtgga gctccacgca gacgccgcac
     2041 tacgtccacc gggcgcttgc gaaggtcctc gagattccgg cgccgcggat ccgcgtgatc
     2101 gccacgccca acggcggcgg tttcggcgga aagtcggatc ccttcaacca cgaaatcgtc
     2161 gtggcgcacc tcgcccgcgt gacgggacgg ccggtcaaaa tctgcctgac ccgcgaagag
     2221 gtcttctact gccaccgcgg ccgccacccg acgttgatgc gcgtcaagac gggcgtgaag
     2281 cgcgacgggg cggtcaccgc gctgcacttc cagactgtgc tggacggcgg cgggtacggg
     2341 tcctacggag tcgcgtcgac ttattacacc ggcgcgctcc agaccgtgac gtatcgcgtc
     2401 ccgagctacc ggttcgaagg ggctcgcgtc ttcacgaaca agccgccgtg cggccccaag
     2461 cgcgggcacg gcaccccgca gccgcgctat gcgctcgagg tgcatctgga caagatcgcg
     2521 tgcgacctcg ggctcgaccc ggcggaaatg cgaaagtcac agctcgtccc gccgaactcg
     2581 gtcaccgcga acttcctgcg catcggctcg atgggtctcg gcgcctgcat cgacaaggtc
     2641 gtcgccgcgt ccggatggcg cgaacggcgc gggaagctgc ctttcggccg cggcctcggg
     2701 ctcgcgtgct cttcctacat ctgcggcgcc ggactcccga tctactggaa ctcgatgccg
     2761 cagtccggcg tccagctgaa gctcgaccgc agcgggggag cgacggtctt ctgcggttcg
     2821 atcgacatcg gccaggggag cgactcggtc ctcgcgtacg tcgtcgggga agtcctcggg
     2881 ctcgatccgt tcgacatccg cgtcgtcacc gccgacaccg acctgacccc ggtcgatctc
     2941 ggctcctatt cgtcacgcgt cacgctgatg acgggcaacg ctgccctcca ggcggccgag
     3001 cgagcgcgcg agctcctcgt cgatgcggtc gccgccaagc tcgcgatccc gaaggcccgc
     3061 gtcgctctcg cggagggtcg cgtctttgac gtcgaggagc ccggaacggg cctgacattc
     3121 gccgacgcgg tcgtcctcgc ggagtcgaaa ttcggcacga tcgggacggt cggctcctac
     3181 acgccgccgc catcccccgg ccgctaccgc ggcgcgggag tcggtccctc gcccgcgtac
     3241 tcgtattccg ccgcggtcgt cgaggtggac gtcgacccgg aaaccgggat ctacaccgtc
     3301 ccgaaggtgt ggatcgcgca cgacgtcgga aagtgcatca acccggttct cgtcgtgggg
     3361 caggttgagg gctccgtcta catgggtctc ggcgaggcca tgatggaaga gatggcgtat
     3421 cgctccggcg tgaatgcgaa cgtcgtccac aagttcccct ctctcctcga gtacaagagc
     3481 ccgacgacga aggagacgtc cgaagtcgtc acttttctca tcgaggatcc cgatcccaac
     3541 ggtccgttcg gcgcgaagga ggtcggacag ggtccgctcc tcccgatccc gccggccgtc
     3601 tcgaacgctc tcttcgacgc ggtcggcgtc cgcgtggacg aaatcccggc gactccggag
     3661 aaggttcatg cggccttgaa gctcggggcg gccggacagg tggcgcgcgt cgggcctgtt
     3721 cggtttcccg aaattccgta tccgccgccg atgcgcgtgc tcactccgga agaggggggg
     3781 gatggccggg cagtcgaagc agcggtgacc ggtgctggcg gcgtgagata gccatgatga
     3841 ggcttccgag attccgatac ctctcgccgc gcacggtcgc ggaggcggtc gcgatgcgtg
     3901 ccgatgccgg gcccgacggc gcgtacgtcg ccggcggcac cgacctctac ccgaacatga
     3961 aacggcgcca gcagactccc agggtcgtcg tcgggttgtc gcgcgtcgcg tcgctctccc
     4021 ggatccggga gagtgacggc cggatcctgc tcggcgccgg gctcgtcctc gcggaggtcg
     4081 agaacgactc gcaggtgcgg gcgggctatc ccgggctggc gcatgcggta tcggagatct
     4141 ccaccccgcc gcttcgcaac atggggacgc tcggcggcaa cctcatgctc gacacgcggt
     4201 gcaactatta cgaccagaat tacgagtggc ggaaggcgat cgacttctgc atgaagaagg
     4261 acgggcagat ctgctgggtc gctcccggct cgccgaagtg tctggcggtg cagtcggcgg
     4321 acaccgtgcc cgtcttgatc gcgatgggcg cccgggcggt gctggcgtcg ccggaagggg
     4381 aacgggagat cgccgtcgaa gacctctacc gcaacgacgg aatcgagtac gtgaccaaga
     4441 ggccgggcga gctcctgacg gaagtcgtgc tgccgcggga gaacggctgg cgtgcgagct
     4501 accgcaagct tcgccgccgc gggtctttcg atttcccggt gctctcggtc ggtgccgcgg
     4561 tctggaggga cggcggcgtc gtgacggccg cccgtctcgt gctgggaggc gtggcttcgg
     4621 cgcccgtccg cctccccgcc tccgaggcgg ccctcgtcgg ccgtccgctc gacgacgctt
     4681 cgatcgcgga cgccgcggcc gccgcggcag gtccatcgcg cccgatggac aacacggact
     4741 t
//
LOCUS       DASWAV010000627         5469 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U2665
            k141_277657, whole genome shotgun sequence.
ACCESSION   DASWAV010000627 DASWAV010000000
VERSION     DASWAV010000627.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960316
            Sequence Read Archive: SRR10260007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 5469)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5469)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:47:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,929
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5469
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_277657"
                     /isolate="SMAG_U2665"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.36444 N 132.31889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(15..1619)
                     /locus_tag="VF958_12995"
     CDS             complement(15..1619)
                     /locus_tag="VF958_12995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9150072.1"
                     /translation="MTSGPPRPTLEEARRRLRELGYLQGPVERFVFRRAIEHVSGLVV
                     PVVAVIAAALAAAETAAVASSQFRYGGSPRAAVFLFLQLGIVALLPAGVFAAILSPAA
                     ARSRRPGRGAAAFALVASGAVLLVWLAGTWRLGAERAPAVLLWGIPATLGALLAGVTT
                     RGAFLARAFARSGRLPERRRRALLLFAAAGALSLAAIFLATRREAERPAPPLPSPRAA
                     MLAIVGIDGLSLDTRQDPGERAFASLFSQGATGRWEAERASPAEIWTDLATGVPAARH
                     GVRALEWVRPWGLPALRPPFGTVWYFRGVGLAAGSVARAPVSAGERRSPAFWEVAASA
                     GLSSLAVGWWASGPWPGADVVENREVLARSSSGLDADAVAIAEFERRAEGKKIAAVYL
                     PGADILRGDPPARRAALARTWLFLDRMIARARSGAIALVVIAADSHPAPGALGRMVVF
                     EKGVATGSAAIRPFDVAPTLLARAGVPAARDLAGRPASGLFSPGSLETATVATYGDRI
                     APVAAARGRETDREYLEKLKSLGYLN"
     gene            complement(1616..2527)
                     /locus_tag="VF958_13000"
     CDS             complement(1616..2527)
                     /locus_tag="VF958_13000"
                     /inference="COORDINATES: protein motif:HMM:NF012568.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HEX9150073.1"
                     /translation="MARNRSPSAAGRSAFEQYAAASRPGLEGAIVRNLPSPARGAAPL
                     FEAVEYAVLSPGKRIRPLVAMAAGELVRARPAGPLSVAVAVEYAHSASLVLDDLPSMD
                     NAARRRGRSALHLVFGVAIAELASVTLLSRAFETIAKEPAIGGAVRTRIVGELAAAVG
                     AAGCCGGQAADLASDPGSIGLEDLESIHARKTGALFVAAVRGGALAGGATEAALEALT
                     LYARNLGLAFQITDDLLDEEGDPETLGKDVRKDAHRANFVSALGAESCRALVAELLET
                     AVAALAPFRSRAAVLADLARFVRDRRS"
     gene            complement(2591..2932)
                     /locus_tag="VF958_13005"
     CDS             complement(2591..2932)
                     /locus_tag="VF958_13005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002614829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HEX9150074.1"
                     /translation="MKSNVREAQGVSIVDLKGKITIGSGDVQLREIINGLVEKGKKNI
                     LINMQEVTTIDSSGIGELVGCYTSVTNKGGKLKLLHLPPKITDVLTVTQLITVFDVYE
                     SESEALATYAA"
     gene            complement(2929..3396)
                     /locus_tag="VF958_13010"
     CDS             complement(2929..3396)
                     /locus_tag="VF958_13010"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF024970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX9150075.1"
                     /translation="MRAPPSSNDAGPIRMSLSSRFENIELAQHLCGKLLEGREVSEET
                     RHWLLMALREALANAIKHGNRQDLSKRIHLEMDVVGQDLRISIRDEGEGFDPAAVDDP
                     LAPENRLKTSGRGIFYMKTFMDDVRFRKLEAGGMEITLTKNLSTGKEKEGNRT"
     gene            3513..4733
                     /locus_tag="VF958_13015"
     CDS             3513..4733
                     /locus_tag="VF958_13015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9150076.1"
                     /translation="MKRNRRVSAFLALSAVLLCGASTLLPARIREVSRLVEDVRGRRF
                     DNPVPASEINSGALRKFLKAKLADSFPAPPEETLSSLVVLGLIDRTPDLLERLLDFYT
                     SQVIAFYDPEPHRFFVVRGGARNALEEAGGGDLSERLLFSHELTHALQDQALRLDSRF
                     KSLRDDGDRALALQCLLEGEATLVMVRVALRDLPGADSAAEEALAPLLSAGALEQANA
                     PKDLPPFFVKQLFFPYVEGTAYVRRAVARGGWKEVDRLWTDPPVSTAEILHGDLRVAP
                     AEDLLPPDPSRLAPARARYLYSDTIGEWAIRFLLERSLPEAEASAAAAGWRGDRLAFF
                     VAGNDYSYLWRIRFESGGAAERFAAAFEKTRATRLNPPRVSRTGSDVVISLGFAEAPA
                     LPGLPAVRLLAPRG"
     gene            complement(4708..>5469)
                     /locus_tag="VF958_13020"
     CDS             complement(4708..>5469)
                     /locus_tag="VF958_13020"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014566.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane glutamic
                     endopeptidase"
                     /protein_id="HEX9150077.1"
                     /translation="AGITLSFARARSRAVFAEDGFHEPGRRTLAVALLASVLLLTVAI
                     PFAGGFAGAQPETENLTVVSLFAVHAILIFFLACYYGLSGRHSLADFLKIRSPRPVAD
                     LSAGIMIGAAGWLLTVLAALGAVGIWYLLRRSTPGTEENAAVSPTILWIVSQPVWVKV
                     AIVVSAMVVEELFFRSFLQTRVGPLAATLMFTAAHGVYGQPLVLIGILVISAVLSVTF
                     ALYGNVLPCIVAHGIFDSIQMFVLIPLVTRAAQGA"
BASE COUNT          920 a         1921 c         1851 g          777 t
ORIGIN      
        1 cgtgagaggt tacgtcaatt cagatagccg agcgacttca acttctccag gtactcgcga
       61 tcggtctcgc gaccccgcgc cgcggcgacc ggagcgatcc gatcgccata cgtggccacg
      121 gtcgcggttt ccagggagcc cggcgagaac aggccggacg ccggccggcc ggccaggtct
      181 cgcgccgccg gaaccccggc tcgcgcaagg agcgtggggg cgacgtcgaa aggtcggatg
      241 gcggccgatc ccgtggcgac gcccttctca aagacgacca ttcggccgag cgcgcccggg
      301 gccgggtggc tgtccgccgc gatcacgacg agggcgatcg cccccgagcg cgcccgggca
      361 atcatccgat cgaggaagag ccaggtgcgc gcgagagccg cgcgccgcgc cggcggatcg
      421 ccccggagga tgtcggcgcc ggggaggtac accgcggcta tcttctttcc ctccgcgcgt
      481 cgctcgaact ccgcgattgc caccgcgtcg gcgtcgagac cgctcgaaga ccgcgcgagc
      541 acctcgcggt tctcgacgac gtcggccccc ggccacgggc cggatgccca ccacccgact
      601 gcaagcgacg aaagacccgc ggacgcagcg acctcccaga acgcgggact gcgcctctcc
      661 ccggcggaga ccggcgcgcg cgcaaccgaa cccgccgcga gaccgacgcc gcggaagtac
      721 cacacggtgc cgaacggagg acgcaatgcc gggaggcccc acggccgcac ccactcgagc
      781 gcacgcacgc cgtgccgcgc cgccggcact ccggtcgcga gatccgtcca gatctccgcg
      841 ggcgaggcgc gttcggcctc ccaccggccg gtggcaccct gggaaaagag actcgcgaac
      901 gctcgctcgc cggggtcctg ccgcgtgtcg agcgagaggc cgtcgatgcc gacgatggcg
      961 agcatcgcgg cccgcggcga gggaagtggc ggagccgggc gctcggcctc ccgccgcgtt
     1021 gcgagaaaaa tcgccgcaag cgagagcgct cctgccgcgg cgaagaggag caaggcgcgc
     1081 cgccgtcgct ccgggagccg tcccgatcgc gcgaacgcgc gcgcaagaaa cgccccgcgc
     1141 gtggtcacgc cggccagcag tgcgccgaga gtcgccggaa tcccccagag gaggaccgcg
     1201 ggcgcgcgct ccgcgccgag gcgccacgtt cccgccagcc agacgaggag cacggcaccc
     1261 gaagcaacga gtgcgaaggc cgcggcgcct cgtcccggac gtcgcgatcg cgcggctgcc
     1321 ggcgaaagga tggccgcgaa gactcccgcc ggcaggagcg ccacgatgcc gagctggaga
     1381 aagagaaaga cggcggcgcg gggcgatccc ccgtagcgga actgagacga cgccacggcc
     1441 gcggtttccg cggccgcgag agccgccgcg atgacggcga ccacggggac gacgagaccg
     1501 gagacgtgct cgatcgcccg gcggaagacg aaccgctcga ccggcccctg gaggtacccg
     1561 agctcgcgca gtcgccggcg cgcttcctcc agcgtcggcc gtgggggacc ggaggtcacg
     1621 aacgccggtc gcgcacgaaa cgcgcgaggt ccgcgagcac cgcggcgcgg ctgcgaaacg
     1681 gcgccagagc ggcaactgcc gtttcgagga gctcggcgac gagagcccgg caagactcgg
     1741 cgccgagggc gctcacgaag ttcgcgcggt gggcgtcttt gcggacatcc tttccgagtg
     1801 tctccgggtc gccctcttcg tcgagcagat cgtccgtgat ctgaaacgcg aggcccaggt
     1861 ttctcgcgta cagcgtgagc gcctcgagtg ccgcttcggt cgcaccgccc gccagcgccc
     1921 cgccccggac cgccgcgacg aagagcgccc cggtctttcg tgcgtggatc gactcgaggt
     1981 cttcgaggcc gatcgagccc gggtccgacg ccaggtccgc cgcctggccg ccgcagcacc
     2041 ccgcggcgcc caccgccgcc gcaagctccc ccacgatccg cgtccgtacc gcccccccga
     2101 tcgccggctc tttcgcgatc gtttcgaagg cgcgggaaag gagcgtcacc gaggcgagtt
     2161 ccgcgatcgc cactccgaag acgaggtgga gcgcggagcg cccgcgccgc cgcgcggcgt
     2221 tgtccatcga cggaaggtcg tctaagacga gcgaggccga gtgcgcgtat tcgacggcca
     2281 cggccaccga aagggggccg gccggccgcg cgcggacgag ctccccggca gccatcgcca
     2341 ccagcggccg gatccgtttc ccgggagaga ggacggcgta ttcgacggcc tcgaagagcg
     2401 gggctgcgcc gcgcgcgggc gacgggagat tgcggacgat cgccccttcg agccccggac
     2461 gcgacgccgc ggcgtattgc tcgaacgcgc tccggccggc ggccgaagga gagcggttgc
     2521 gggccacgac agccgggttc tcagtcctca gctctcgaac tgaggactga gcgctggacc
     2581 acccggcgac ctaggcggcg tacgtcgcca gggcctctga ctccgactcg tagacgtcga
     2641 acactgtaat gagctgggtg acggtgagca cgtcggtgat cttcggcgga aggtggagga
     2701 gcttcagctt cccacccttg ttcgtcacgg aggtgtaaca gccgacgagc tcgccgatcc
     2761 cggacgaatc gatggtcgtc acttcctgca tgttgatcag gatgtttttc ttgcccttct
     2821 cgacgagacc gttgatgatc tcgcgcaatt ggacgtcccc ggagccgatc gtgatcttcc
     2881 ccttcaggtc gacgatcgag actccctggg cttcgcgcac gttcgacttc atgtccggtt
     2941 tccctccttc tccttacccg tgctcagatt cttggtcagg gtgatttcca ttccgcccgc
     3001 ctcgagcttt cggaaacgga cgtcgtccat gaacgtcttc atatagaaga tcccgcgccc
     3061 cgacgttttc aaacgatttt cgggcgccag cggatcgtcg accgccgccg ggtcgaatcc
     3121 ttccccctcg tcgcgaatcg agatccgcag gtcctgtccc accacgtcca tctccaggtg
     3181 aatgcgcttc gagaggtcct gacggttgcc gtgcttgatc gcgttcgcga gagcttcgcg
     3241 cagcgccatc agcagccagt gcctggtctc ttcggaaacc tccctcccct cgaggagttt
     3301 gccacaaagg tgttgcgcca actcgatgtt ctcgaatcgg ctggaaagcg acatccggat
     3361 gggaccggcg tcgttggacg aaggcggcgc tctcattcac tctgtcgcgg caaaatcggc
     3421 gcatattaag ccataaaccg agccggaaga cgggaaacgg aaacaggaaa cgggaaaaag
     3481 gcgatcaacg cccgcatgct aacctgccgt tgatgaagcg aaaccggcgc gtctccgcgt
     3541 ttctggcgtt gtcggcagtc ctcctctgcg gcgcgtcgac gctgctcccg gccaggatcc
     3601 gcgaagtttc ccggctcgtg gaagacgtgc gcggccggcg gttcgacaac ccggtcccgg
     3661 cgtcggagat caattcagga gcgttgcgga agttcctgaa ggccaagctc gccgactcct
     3721 tccctgcacc gccggaggag accctgtcct ccctcgtcgt gctgggcctg atcgacagga
     3781 caccggacct gctcgaacgg ctcctcgact tctacacctc gcaggtgatc gccttctacg
     3841 atcccgagcc gcaccgcttc ttcgtcgttc ggggcggagc gcgcaacgcc ctcgaggagg
     3901 cgggtggcgg agatctgtcc gagcggctgc tcttctccca cgagctcacg cacgcactcc
     3961 aggatcaggc gcttcggctc gacagccggt tcaagtccct gcgcgacgac ggcgaccgcg
     4021 cgctggccct ccagtgtctg ctcgagggcg aggcgaccct cgtcatggtg cgggtcgcgc
     4081 tccgggacct gcccggcgcg gacagcgctg ccgaggaagc gctcgcgccg ctcctttcgg
     4141 caggagcact cgagcaggcg aacgccccga aggacctgcc gcccttcttc gtgaagcagc
     4201 tcttctttcc ctatgtcgag ggcaccgcgt acgtgcgtcg tgcggtcgcg cgcggcggat
     4261 ggaaggaagt cgaccggctg tggaccgacc ctccggtctc gacagccgaa atcctccacg
     4321 gggacctccg cgtcgcgccg gcggaggatc tccttccgcc ggatccgtct cgcctcgccc
     4381 ccgcgcgcgc tcgttacctc tattcggaca cgatcgggga gtgggcgatt cgcttcctcc
     4441 tcgagcgcag cctgccggag gccgaggcgt cggcggccgc cgcgggatgg cggggcgatc
     4501 gcctcgcgtt cttcgtcgcg ggcaacgact actcctacct ctggcgcatt cgcttcgagt
     4561 cgggcggagc cgccgagcga ttcgccgccg cgttcgaaaa gacccgcgcg acccgtctta
     4621 atcccccccg ggtctcgcga acgggctccg acgtggtcat ctccctgggg ttcgcggagg
     4681 cgccggcgct gccgggcttg ccagccgtca ggctccttgc gccgcgcggg tgaccagggg
     4741 aatcagcacg aacatctgaa tcgagtcgaa gatgccgtgc gcgacgatgc acggcaggac
     4801 gtttccatac agggcgaacg tcaccgacag gacggcggag atcacgagga tcccgatcag
     4861 gacgagaggc tgtccgtaga cgccgtgcgc ggccgtgaac atcagggtcg cggcaagagg
     4921 tcccacccgc gtctggagga aggagcggaa gaagagctct tcgacaacca tcgccgagac
     4981 gacgatcgcc accttcaccc agaccggctg cgagacgatc cagaggattg tcggcgaaac
     5041 cgcggcgttc tcctccgtcc cgggggtcga cctccggagg aggtaccaga ttcccacggc
     5101 cccgagggcg gccagaacgg tcaggagcca gcccgccgcg ccgatcatga tccccgccga
     5161 aagatccgcc acggggcggg ggctccggat cttcaggaag tcggcgagag agtggcgacc
     5221 cgacagaccg tagtagcacg cgaggaagaa gatcaggatc gcgtggaccg cgaagagcga
     5281 aacgacggtc agattctcgg tctccggctg cgcgcccgcg aaaccgccgg caaacgggat
     5341 cgcaaccgtg aggaggagga ccgacgccag gagcgcaacg gcgagagttc ggcgacccgg
     5401 ctcgtgaaag ccgtcttcgg caaagaccgc ccgcgaccgc gcgcgagcga aagagagggt
     5461 gatccccgc
//
LOCUS       DASWAV010000628         4971 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U2665
            k141_717150, whole genome shotgun sequence.
ACCESSION   DASWAV010000628 DASWAV010000000
VERSION     DASWAV010000628.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960316
            Sequence Read Archive: SRR10260007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 4971)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4971)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:47:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,929
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4971
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_717150"
                     /isolate="SMAG_U2665"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.36444 N 132.31889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(89..910)
                     /locus_tag="VF958_13025"
     CDS             complement(89..910)
                     /locus_tag="VF958_13025"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: protein motif:HMM:NF013327.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HEX9150078.1"
                     /translation="MKRELAAGISIPIVLAVVFLAPPIAFNLLVAAIALGALWEFYRL
                     AEKTGLPVAKTVGMLAGAALLVWWASFWGYAYSPHPGITVALGALLTFVFLASLAPLF
                     ARVEIPAALGGAAGTVFGVLSIAMPATAMCFLRIVSPRAVLLLFVLVWGCDSFAYYFG
                     KNFGKRKLAPRVSPNKTWEGTIAGFVGGTLIGAAAGTWWLYPELGPLLGALAGALATS
                     AGQLGDLVESLWKRGAGVKDSGNFLPGHGGFYDRIDSLLYAAPVLAFFIGGSTPP"
     gene            complement(938..1759)
                     /locus_tag="VF958_13030"
     CDS             complement(938..1759)
                     /locus_tag="VF958_13030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010587773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HEX9150079.1"
                     /translation="MIDVDVLAEPGSSERVLLDEIDLSRLPRHIAVIMDGNGRWAKQR
                     GFPRVEGHRAGIASVREVVETCARLELDALTLYAFSVENWKRPRFEIVTLMSLLKEYL
                     NKELANLLKNDIRFRVVGRMNELDASVQKELSRGLAATSSCRGMTFNIALNYGGRTEI
                     VDACRSLAQDVAAGRLTPEQIDEETLGSRLGTAGVPDPDLLIRTSGEMRVSNFLLWQI
                     AYSEIWVTPTLWPDFRKRHLFEAILDFQKRERRYGGVIDAGRASEHGEAAADGRR"
     gene            complement(1756..2526)
                     /gene="truA"
                     /locus_tag="VF958_13035"
     CDS             complement(1756..2526)
                     /gene="truA"
                     /locus_tag="VF958_13035"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HEX9150080.1"
                     /translation="MRDDGPYRYRAVIAYVGTDFHGWQIQRNASRTVQAALEEALARF
                     DGAAVRAVAAGRTDAGVHADGQVVHFDLSGSREPREVRDGVNALLPRDVRILQAARAS
                     AEFHARRDALWKDYLYRWSRAEVIAPKDAPFLAPLSRNADAGRMAAAAEHLPGRRDFG
                     VFGVRRSTGDSVRTLHSVAIEERGVEVRALFRGDGFLRGMVRTICGLLADIGRGRLPP
                     DRMREILETGNRALLAQKAPAGGLTLVKVEYPRVKLNP"
     gene            complement(2562..3950)
                     /locus_tag="VF958_13040"
     CDS             complement(2562..3950)
                     /locus_tag="VF958_13040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016758 - hexosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase 87 family protein"
                     /protein_id="HEX9150081.1"
                     /translation="MHSRPGRGARLSPWLAFAAGESVFLGFALLPDARRHLAAYLLLF
                     LAGSLLSISAARSLSASSLRFLLVCAAVFRLTLLFRTPDLSEDVYRYLWDGRVATAGN
                     SPYALAPADARPDFFPALRRQVSYPELRTVYPPVAQAAFRAVASSPFPGIFAMKGLFS
                     AADVAVVALLFASGGAGARFAAALYAFHPLPVTETAGQGHLDSLGVALLLACVVFLAR
                     QRPRRLSAGIAFALAVLVKYVPLAAAPALLRRGRLWFAATTIAATAVVWMLATRGGVS
                     PAGGLGPYATRWEFNSVLYPAVSAAVDRLDLPQRAKSAYIRWKEKREYRPWMQKIFPY
                     FYTGFFARALLGIALGVALLIIGVSVKEVELAVFVSIAALLLASPTLHPWYLLWVLPF
                     AAKRREPAFLFLCTAAPIAYGLLHPLAGWTPLAIRVVEYGPFAVLLGARLYVAAKRRG
                     QLNSLAPLAGRG"
     gene            3945..>4971
                     /locus_tag="VF958_13045"
     CDS             3945..>4971
                     /locus_tag="VF958_13045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015351853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose synthase family protein"
                     /protein_id="HEX9150082.1"
                     /translation="MHIQIATLVAYYVVLTLLAVYGVHRAFMVRLYFRHRGDDPLPAG
                     DLERLPVVTVQLPLYNEVYVVERLLDAVMALDYPKELLEVQVLDDSTDETHEVARVAV
                     ERYRSRGYDVVHLSRDHRSGYKAGALQAGLERARGEFLLIFDADFLPQPDMLRRCLPH
                     FGNREVGMVQARWDHLNREFSLLTRVQSIFLDGHFVIEHTARNRSGRFFNFNGTAGIW
                     RRRCLQEAGGWQADTLTEDLDVSYRAQLAGWRFVYLKDVTVPAELPVDINGFKTQQHR
                     WTKGSIQTARKLLPAILRSSFPWKVKLEAVFHLTSNFSYLLVVLLALLLLPAIVIREE
                     IGWRKLAV"
BASE COUNT          881 a         1711 c         1680 g          699 t
ORIGIN      
        1 attatacgaa ggcggagacg tgtcagaccc gggtcgcccc catgctgcga gatcaccatg
       61 ggatggtgga tcgcggccat ttgctctctt acggcggcgt cgagccgccg ataaaaaacg
      121 ccaggaccgg cgccgcgtaa aggagcgaat cgatccggtc gtagaaaccg ccgtggcccg
      181 gaaggaagtt ccccgagtcc ttgacgccgg cgccccgctt ccacagcgac tcgacgaggt
      241 cgccgagctg cccggccgac gtcgcgagcg ccccggcgag cgcgccgagg agcggcccca
      301 gctccgggta gagccaccac gtcccggccg cggcgccgat caaggtgccg ccgacgaagc
      361 ccgcgatcgt tccctcccac gtcttgttcg gcgagacgcg cggcgcgagc ttgcgcttcc
      421 cgaagttctt tccgaagtaa taggcgaagg agtcgcagcc ccagacgaga acgaagagaa
      481 gaagcaccgc gcgcggagag acgatccgaa ggaagcacat cgcggtcgcc ggcatcgcaa
      541 tggagagaac gccaaagacg gttcccgcgg cgcctccgag cgcggccggt atctcgaccc
      601 tggcgaagag cggcgcgaga gatgccagga agacgaacgt gaggagcgcg ccgagcgcga
      661 cggtaatccc cggatgcggc gagtacgcgt atccccagaa cgaggcccac cagacgagga
      721 gcgccgcacc cgcgagcatc ccgacggtct tcgccacggg gagtcccgtc ttctcggcga
      781 gccggtagaa ctcccaaagc gcgccgaggg caatcgccgc gacgagcagg ttgaacgcaa
      841 tcggtggcgc gagaaagacg accgcgagaa cgatcgggat gctgatgccc gcggcgagtt
      901 cccttttcaa ggccacccgt cgggtcccgt cgaccgatta ccgtcggccg tctgcggcgg
      961 cctcgccgtg ttcggacgcc cggccggcgt cgatcactcc gccataccgg cgctcgcgct
     1021 tctggaagtc gaggatcgcc tcgaagaggt ggcgcttgcg gaagtccggc cagagggtgg
     1081 gcgtcaccca gatctcgctg taggcgattt gccagaggag gaagttggag acgcgcatct
     1141 cgccggacgt gcggatgagg aggtccgggt ccggcacgcc cgccgtgccg agccgggaac
     1201 ccagcgtctc ctcgtcgatc tgctcgggcg tcaaccgccc cgcggcgacg tcctgcgcga
     1261 gcgagcggca tgcatcgacg atctcggtcc ggccgccgta gttcaaagcg atgttgaacg
     1321 tcattccgcg gcacgacgac gtggccgcga gaccccgcga cagctccttc tgcaccgagg
     1381 cgtcgagctc gttcatccgc ccgacgacgc ggaagcggat gtcgttcttc aagaggttcg
     1441 cgagctcctt gttcaggtac tccttcagga gcgacatgag cgtgacgatc tcgaaccgcg
     1501 ggcgtttcca gttctcgacg gagaacgcgt agagcgtcaa cgcgtcgagc tcgagccggg
     1561 cgcaggtctc gaccacttcc cgcaccgacg cgatcccggc gcggtgccct tcgacgcgcg
     1621 ggaatccgcg ctgcttcgcc cagcggccgt tgccgtccat gatcacggcg atgtgacgcg
     1681 ggagccgcga gaggtcgatc tcgtctaaga gcacgcgctc ggaggagccg ggttccgcga
     1741 gaacgtcgac gtcgatcacg gattcagttt aaccctcgga tactcgactt tcacgagggt
     1801 gagcccgccc gccggcgcct tctgggcgag cagggctcgg tttccggtct cgaggatttc
     1861 cctcatccgg tcgggcggga ggcgcccgcg gccgatgtcg gcgagcagtc cgcagatcgt
     1921 gcgcaccatg ccgcgcagga agccgtcccc ccggaagagc gcgcggactt ccacgccccg
     1981 ttcctcgatc gcgaccgagt ggagcgtccg cacggagtcc ccggtcgacc gcctcacccc
     2041 gaatacgccg aagtcgcgtc ggcccggcag gtgctccgcc gccgccgcca tccgccccgc
     2101 atccgcgttg cgcgacagag gggcgagaaa gggcgcgtcc ttcggcgcga ttacctcggc
     2161 tcggctccag cggtagaggt agtccttcca gagagcgtcg cggcgcgcgt ggaactcggc
     2221 ggacgcgcgc gcagcctgga ggattcgcac gtcccgggga aggagcgcgt tgactccgtc
     2281 cctcacctcg cgcggctccc gcgaaccgga gagatcgaag tgaacgacct gcccgtccgc
     2341 gtgcactccg gcgtccgtgc gtccggcggc gaccgcccgc actgcggcgc cgtcgaagcg
     2401 cgcgagagcc tcctcgagtg ccgcctgcac cgtgcgggac gcgttgcgct ggatctgcca
     2461 gccgtggaag tccgtgccga cgtaggcgat gacggcgcgg taccggtaag gaccgtcgtc
     2521 cctcaccgag cgttactccg ttcgaccacc gcccctcacc cttaccctct ccccgcaagc
     2581 ggggcgaggg agttcaattg gccgcgacgt ttcgccgcca cgtaaagcct cgccccgagc
     2641 agcaccgcga acggcccgta ctccacgacc cggatcgcga gcggcgtcca gccggcgagc
     2701 ggatggagca ggccatacgc gatcggcgcc gccgtgcaga ggaaaaggaa cgcgggctcg
     2761 cggcgtttcg cggcgaacgg cagcacccac aggaggtacc aggggtggag cgtcggcgac
     2821 gcgaggagca gcgcggcgat ggaaacgaag acggcgagct ccacctcctt cacgctcacg
     2881 ccgatgatca acagggcgac gccgagcgcg atcccgagca gcgcgcgagc gaagaacccc
     2941 gtgtagaagt acggaaagat cttctgcatc caggggcggt actcgcgctt ctccttccac
     3001 cgaatgtagg cgctcttcgc tctctgcgga aggtccaggc gatccaccgc cgccgacacc
     3061 gcgggataga gcacggagtt gaactcccag cgggtcgcgt acggcccgag gccgccggcg
     3121 ggcgaaactc cgccgcgcgt cgccagcatc caaacgacgg ccgtcgctgc aatcgtcgtc
     3181 gcggcgaacc acaaccgccc ccgccgcagg agcgccggag ccgccgcgag cggcacgtat
     3241 ttcacgagca cggccagagc gaacgcgatt cccgcggaga gccgacgcgg gcgttgccgc
     3301 gcgagaaaca cgacgcacgc caggaggagg gccacgccca gggagtcgag atgcccctgt
     3361 cccgccgtct cggtgaccgg cagcggatga aaagcataga gcgcggcggc gaaccgcgct
     3421 ccggcgcctc cgctcgcgaa gaggagcgcg acgaccgcga cgtcggccgc cgagaagagt
     3481 cccttcatcg cgaagatccc gggaaaggga ctcgaggcga ccgcgcggaa cgcggcctgc
     3541 gcgaccggcg gatagacggt gcggagctcc ggatacgaga cctgccggcg cagcgccgga
     3601 aagaagtccg gccgcgcatc cgcgggcgcg agagcgtacg gcgagttccc ggcggtcgcg
     3661 acgcgcccgt cccacagata ccgatagacg tcttccgaga gatcgggcgt tcggaagagc
     3721 agagtcaggc ggaagacggc cgcgcagacg agaaggaagc ggagggatga agcggataaa
     3781 ctgcgcgccg cggaaatcga gaggagggat ccggccagaa aaaggaggag ataagcggcc
     3841 aggtgacgcc tcgcgtcggg gagcagcgca aacccgagaa agacgctctc tcccgccgcg
     3901 aaagcgagcc agggcgagag ccgggctccc cttccggggc gggaatgcac atccagatcg
     3961 cgaccctcgt cgcctactac gtcgtcctca cgttgctcgc ggtgtacggg gtccaccgag
     4021 cgttcatggt gcgtctctac ttccgccacc gcggcgacga tccgctcccc gccggcgatc
     4081 tcgagcggct tcccgtcgtc accgttcagc tccctcttta caacgaagtc tacgtcgtcg
     4141 agcggctgct cgacgccgtg atggcgctcg actatccgaa ggagctcctc gaggtccagg
     4201 tcctcgacga ttccaccgac gagacgcacg aagtcgcgcg ggtggcggtc gagcgctacc
     4261 gcagccgcgg atacgacgtc gtgcacctct cgcgcgatca ccggagcggc tacaaggcgg
     4321 gggccctgca ggccggcctc gaacgcgcgc gcggcgaatt cctgctgatc ttcgacgcgg
     4381 atttcctccc gcagcccgac atgctccggc ggtgcctgcc ccacttcggc aatcgtgagg
     4441 ttggaatggt ccaggcgcgt tgggaccacc tgaaccgcga gttttcgctc ctgacgcggg
     4501 tccagtcgat cttcctcgac gggcacttcg tcatcgagca cacggcgcgc aaccgttcgg
     4561 gtcgcttctt caacttcaac ggcactgcgg ggatctggcg gcgaaggtgc ctccaggaag
     4621 ccggaggctg gcaggccgac acgctgacgg aagacctcga cgtgtcgtat cgggcgcagc
     4681 tcgcgggctg gcgcttcgtt tacctcaagg acgtcacggt gccggccgag ttgcccgtgg
     4741 acatcaacgg cttcaagacc cagcagcacc gctggacgaa gggttcgatc cagaccgccc
     4801 gcaagctcct gcccgcgatc ctcaggagct cgtttccctg gaaggtcaag ctggaggcgg
     4861 tcttccacct gacgagcaac ttctcctacc tgctcgtggt gctgctcgcg ctcctcctcc
     4921 tgcccgccat cgtcatccgg gaggagatcg gctggcgcaa gctcgcggtc c
//
LOCUS       DASWAV010000629         1736 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U2665
            k141_36195, whole genome shotgun sequence.
ACCESSION   DASWAV010000629 DASWAV010000000
VERSION     DASWAV010000629.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960316
            Sequence Read Archive: SRR10260007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 1736)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1736)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:47:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,929
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1736
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_36195"
                     /isolate="SMAG_U2665"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.36444 N 132.31889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(75..>1736)
                     /locus_tag="VF958_13050"
     CDS             complement(75..>1736)
                     /locus_tag="VF958_13050"
                     /inference="COORDINATES: protein motif:HMM:NF012239.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HEX9150083.1"
                     /translation="GITILAKNTAVRYRDTIINIVDTPGHRDFGSEVERILTMVEGIL
                     LLVDASEGPLPQTRFVLKKSIEGEKRPIVLINKIDRADARPLEVEEEIKDLFLSLATH
                     ERHLDFSVLYGSGRLGFASRDPNATSGDLRPLLDAMLEEIPAPAIGVGPFKMIVANTE
                     YSDYVGRQAVGRVFSGTLVRAGSLAVARPDGTLVQTGRAGKIQLFSGIERVEVDEVSA
                     GDICMISGFPAIEIGDTLLDPADPTPLPGIKVDEPTLSMEFRTNDSPMSGRSGRYVTG
                     RHLRERLEKERERNVGLRLERTAEPDASLVLARGELSLSILAETMRREGYEFALGRPQ
                     VILREGENGEVLEPYEELVIDCGEEFTGPLIAAVGERRGELRHIGRHGDRTRIEFTVP
                     SRGLLGFRLEFLSLTRGTGLLNHLFDEYGPHRGPLPNRKKGAMIAKEPGEVTAYALDQ
                     LADRGTFFVKPGDAVYGGQVVGEQVKESDLVVQPCRRKQLTNMRASTADIAVRLNPPR
                     TLTIEQAIEWINDDELVEVTPAAVRVRKRILDHSRRKSEAGSGLH"
BASE COUNT          278 a          581 c          571 g          306 t
ORIGIN      
        1 gggtcaggtc tccacttgcc acttcgcttc gcgaagatgg caagggcctc aactcggaat
       61 cgccgctcgt gcgatcagtg aagacctgac cccgcctctg acttccggcg gctgtggtcc
      121 aggatgcgct tgcgcacgcg caccgcggcg ggagtcacct cgaccaactc gtcgtcgttg
      181 atccactcga tggcctgctc gatcgtcagc gtacgaggcg gattcagtcg gacggcgatg
      241 tcggccgtcg atgcgcgcat gttggtcaac tgctttcgcc ggcagggctg gacgacgagg
      301 tcgctctcct tgacctgctc gccgacgacc tggccgccgt agacggcatc tcccggcttg
      361 acgaagaagg ttccgcgatc cgcgagctga tcgagggcgt acgcggtgac ctcgcccggc
      421 tccttcgcga tcattgcgcc cttctttcgg ttgggcagcg gtccgcggtg cggcccgtac
      481 tcgtcgaaga ggtggttcaa gagaccggtt ccgcgggtca gcgagaggaa ctcgagccga
      541 aagccgagaa gcccgcgcga aggtaccgtg aactcgatgc gcgtccggtc gccgtggcgc
      601 ccgatgtgcc tcaactcgcc gcgtcgttcg cccacggcgg cgatcaacgg accggtgaac
      661 tcctccccgc aatcgatgac gagctcctcg tagggctcca ggacctcacc gttttccccc
      721 tcgcggagaa tcacctgcgg ccggccgagc gcgaactcgt atccctcgcg ccgcatggtc
      781 tcggcgagga tcgagagcga gagctcgccg cgcgcgagca cgagcgaggc atccggctcg
      841 gcggtcctct ccagccggag accaacgttt ctttcccgct ccttctcgag gcgctcccgg
      901 aggtgccggc cggtcacgta cctgcccgag cggccggaca tcggggaatc gttcgtccgg
      961 aactccatcg agagggtcgg ctcgtcgacc ttgatgccgg gcagcggcgt cggatcggcc
     1021 gggtcgagca gcgtgtcgcc gatttcgatc gccggaaaac cggagatcat gcagatgtcg
     1081 ccggcggaaa cctcatcgac ctccacccgc tcgatcccgg aaaagagctg gatctttccg
     1141 gcccgccccg tctgcacgag cgttccgtcg ggccgggcaa cggcgagaga gcccgcgcgc
     1201 acgagggttc ccgagaaaac gcgccctacc gcctggcgcc cgacgtagtc ggagtattcg
     1261 gtgttggcca cgatcatctt gaacgggccg actccgatcg ccggagcggg aatctcctcg
     1321 agcatcgcgt ccaaaagcgg ccggaggtcg cccgaagtgg cgttcgggtc gcggctcgcg
     1381 aagccgagcc ggccggaccc gtagagcacc gagaaatcga gatgcctctc gtgcgtcgcg
     1441 agcgagagga agaggtcttt gatctcctcc tcgacctcga gcggccgcgc gtcggcgcgg
     1501 tcgatcttgt tgatcaggac gatgggtctc ttttccccct cgatcgactt cttcagaacg
     1561 aaccgggtct ggggcagcgg tccttcggac gcgtcgacga gcaggaggat gccctcgacc
     1621 atcgtcagaa tgcgctcgac ctccgagccg aagtcccggt ggcccggcgt gtccacgatg
     1681 ttgatgatcg tgtcccggta gcgcacggcg gtgttcttcg cgaggatcgt gatgcc
//
LOCUS       DASWAV010000630         2112 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U2665
            k141_795361, whole genome shotgun sequence.
ACCESSION   DASWAV010000630 DASWAV010000000
VERSION     DASWAV010000630.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960316
            Sequence Read Archive: SRR10260007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2112)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2112)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:47:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,929
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2112
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_795361"
                     /isolate="SMAG_U2665"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.36444 N 132.31889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..503)
                     /locus_tag="VF958_13055"
     CDS             complement(<1..503)
                     /locus_tag="VF958_13055"
                     /inference="COORDINATES: protein motif:HMM:NF024578.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HEX9150084.1"
                     /translation="MLSTALNGLLLAMLALLPTAAGSPRAGEKPKDKEVIEDRVVVLN
                     DGDVRLDDGDIEVDGDDPIVVHVGRGGFLGVRLVGITDELRSHYGAPKDAGVLVGGVE
                     KDSPASRAGIEVGDVITSVDGNRVDSTGDVSRAVRRKKGGETVDVEVVRGRAARKMTV
                     TLEERKTR"
     gene            622..>2112
                     /locus_tag="VF958_13060"
     CDS             622..>2112
                     /locus_tag="VF958_13060"
                     /inference="COORDINATES: protein motif:HMM:NF021942.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008239 - dipeptidyl-peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0070009 - serine-type aminopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S46 family peptidase"
                     /protein_id="HEX9150085.1"
                     /translation="MRRKSVPAIAFTVALAAPGLFADEGMWMPQQVRELAPELLKMGL
                     KLDPQKLADLAGDPMGAIVSLGGCSASFVSPQGLVITNHHCVYSYLQYNSTPQRDLIE
                     HGFLAKTTAEEVPAAPDARVWVTTSIEDVTDQVLPKSSEKLSDLERYNRVERRRRELV
                     RDCEKEGGVRCTVPSFFEGSKFWRVKQLEIRDVRLVYAPPRGIGNYGGEVDNWMWPRH
                     TGDFGFLRAYVAPDGKPSDYARENVPYQPKHFLKVSTADFDPGDFMMVIGYPGKTYRY
                     ELTDEIKEAQEFDIPTSIRYRKELIRILENAGRTNKDVSIRNAARLRGLLNYLKKYEG
                     TIGAFEKGNLIERRRAEEREIAALVAPDAAAKKRYDESLAEISRLNAAQWKTRERDSL
                     LEWLYSASPMLSQANTVWRLAGERPKNDLDRATGFQDRDLKRLKAGVIRAQRTIEPGS
                     DRAGLRYFLLEAAKLPADQRIPAVDGAFAATGEKDANSAVDKFLDRL"
BASE COUNT          368 a          697 c          705 g          342 t
ORIGIN      
        1 cgcgtcttgc gctcctccag cgtgaccgtc atcttgcgcg ccgcgcgccc gcgcacgacc
       61 tcgacgtcca cggtctcgcc ccccttcttg cgccgcacgg cgcgggagac gtcacccgtc
      121 gagtccacgc ggttgccgtc cacgctcgtg atgacgtcgc cgacttcgat gcctgcgcgg
      181 gaggccgggc tgtctttctc gaccccgccg acgagcacgc cggcgtcctt cggagctccg
      241 tagtgggagc gcagctcgtc tgtgatcccg acgaggcgca ctccgagaaa tcctccgcgg
      301 ccgacgtgca cgacgatcgg gtcgtcgccg tcgacctcga tgtccccgtc gtccaggcgg
      361 acgtctccgt cgttcaggac gacgaccctg tcttcgatca cttccttgtc cttcggtttc
      421 tcgcccgcgc ggggagagcc ggccgcggtc ggaaggagcg ccagcatcgc cagcaggagc
      481 ccgttcaaag ccgtcgaaag cattgcattg atcctccgga tcgggagacg ggcctcgggc
      541 ggaaaggtta cgcggcgggc aatgcgccga tagaattcgg cttctgctcg attccgggcc
      601 gggggcccgg gggaggtcga agtgagacgc aagtccgttc cggcgatcgc attcacggtc
      661 gccctggcgg cgcccgggct tttcgccgac gaagggatgt ggatgcccca gcaggttcgc
      721 gagctcgcgc ccgagctctt gaagatgggg ttgaagctcg acccgcagaa gctcgccgac
      781 ctggccggcg atccgatggg ggccatcgtc tcgctcggcg gctgcagcgc gtcattcgtc
      841 tcgcctcagg gcctcgtcat cacgaaccac cactgcgtct acagctacct gcaatacaac
      901 tcgactccac agagggattt gatcgagcac ggctttcttg cgaaaacgac ggccgaagag
      961 gttccggcgg ctccggacgc gcgtgtgtgg gtcacgactt cgatcgagga cgtcacggac
     1021 caggtgcttc cgaagtcgag cgagaagctg tcggacctcg agaggtacaa ccgggtcgag
     1081 cggcgccgca gggagctggt tcgcgactgc gagaaggagg gcggggtgcg ttgcacggtg
     1141 ccctcgttct tcgaggggtc gaagttctgg agggtgaagc agctcgagat tcgcgacgtc
     1201 cggctcgtct acgcgccgcc gcgcggtatc ggcaactacg gcggcgaggt cgacaactgg
     1261 atgtggccgc ggcacacggg cgacttcggt ttcctgcgcg cgtacgtcgc gcccgacggc
     1321 aagccgtcgg attacgcacg ggagaacgtt ccgtatcagc cgaagcactt cctcaaggtg
     1381 tccacggctg acttcgatcc gggcgacttc atgatggtga tcgggtaccc gggcaaaacg
     1441 tatcgctacg agctcaccga cgagatcaag gaggcgcagg agttcgacat tccgacgtcg
     1501 atccggtacc gcaaggagct catccggatc ctcgagaacg ccggccgcac gaacaaggac
     1561 gtctcgatcc gcaacgctgc gcgcctgcgc ggcctgctca actacctcaa aaagtatgaa
     1621 gggacgattg gcgctttcga gaagggcaac ctgatcgagc ggcggcgggc cgaggagcgg
     1681 gagatcgccg cccttgtcgc acccgacgcc gcagcgaaga aacgctacga cgaatcgctc
     1741 gcggagatct cccggctgaa cgcggcgcag tggaagacgc gcgagcgcga ctccctgctc
     1801 gagtggctct acagcgcctc cccgatgctc tcgcaggcga acacagtctg gcgcctcgcc
     1861 ggagaacgtc cgaagaacga cctcgaccgc gcgacgggct tccaggaccg tgatctgaag
     1921 aggctcaagg cgggcgtcat acgagcccag cgcacgatcg agccgggcag cgaccgcgcc
     1981 gggttgcggt acttcctgct cgaagccgcg aagctgccgg cggatcaacg aattcccgcg
     2041 gtggacggcg cgttcgccgc gaccggagag aaggacgcca actccgcggt cgacaagttc
     2101 ctcgaccggc tc
//
LOCUS       DASWAV010000631         3042 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U2665
            k141_75668, whole genome shotgun sequence.
ACCESSION   DASWAV010000631 DASWAV010000000
VERSION     DASWAV010000631.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960316
            Sequence Read Archive: SRR10260007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 3042)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3042)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:47:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,929
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3042
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_75668"
                     /isolate="SMAG_U2665"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.36444 N 132.31889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..938)
                     /locus_tag="VF958_13065"
     CDS             complement(<1..938)
                     /locus_tag="VF958_13065"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HEX9150086.1"
                     /translation="MKPTTAATTRPMSFRAVRAALARAEHLLMPQEVIASPETRLEYR
                     IDRLLGEGGFGQAYLASRLGRSRSVPRIVCIKASARIDGWLREAYFGQLLDGHPRAIR
                     VYDAFPLIQSDGHVLYCLALEYAPLGDLRAFLHRTGKGWPETTARREIAGILQVLGKL
                     HRGQMLHRDLTPMNVFVCDGRSLKLGDFGIVRHQSDRRGISARTMNAMTAPSEILAGV
                     APKWQARDDVYQVGQLLGMLVKGDAATRVRTHEIRTLPCSDHLKEIVYRCIGERRKRY
                     ESAGELIEALTHRPPPLKVGILRSLKGVHLAFTGILA"
     gene            complement(935..1762)
                     /locus_tag="VF958_13070"
     CDS             complement(935..1762)
                     /locus_tag="VF958_13070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014396292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate lyase/phosphoenolpyruvate mutase
                     family protein"
                     /protein_id="HEX9150087.1"
                     /translation="MTSERRDATRVFRELHQEGLLLLANAWDAGSAHLIESLGAKAIA
                     TTSAGVARSHGYRDGNLLPVPLLAATVAEIARVVNVPITADVEGGYSDDPIAVGDAVA
                     RVIEAGAVGINVEDGGGDPELLCAKIERAKRSGVRLGVDVFVNARVDVYLRKIAPPER
                     RLDETVARAERYRAAGADGIFVPALTDAAEIRAIAAAARLPLNVLVWPGLPPASELQA
                     LGVRRLSAGSWIASAAFAQAASRAAAFLRTGDPGPLTEGAEVYAEINALMDDHPRGR"
     gene            2058..3029
                     /locus_tag="VF958_13075"
     CDS             2058..3029
                     /locus_tag="VF958_13075"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007415849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxidoreductase"
                     /protein_id="HEX9150088.1"
                     /translation="MFRSVSTKEVPMETMKAVRVHQYGGPEVLKYEEAPRPQPGPGEV
                     LVRIRAVGVNPVDWKVREGYMREALAYRMPFVPGWDVSGVVETIGPNVTLLEKGDEVY
                     GYPSIVRNGAYAEYAVVPEAELALKPRSVDHVHAASVPIAALTAWQGLFDVGGLRTEQ
                     KVLIHGGSGGVGSFAVQLARWKGAFIIATASGRNQEFLRNLGADLTIDYEKGRFDHVV
                     CDADVVFDTVGGDTLRRSWKVLKKGGILVSTVEEPSHEDAAAHGVRGAVVDTHADRGE
                     LAEIAKLVDAGMVRPIVEAVFPLNEARHAQELSQTGHARGKIVLQVA"
BASE COUNT          486 a         1028 c         1036 g          492 t
ORIGIN      
        1 gcgaggatgc cggtgaacgc gagatgcacg cccttcagcg acctcaggat accgactttc
       61 agaggaggcg gacgatgggt gagcgcctcg atcagctcgc cggcgctctc gtaccttttt
      121 cggcgctcgc cgatacaccg gtagacgatc tccttcaggt gatcgctgca cggcagtgtc
      181 cggatctcat gggtgcgaac ccgcgtcgcc gcgtcaccct tcaccagcat cccgaggagc
      241 tgcccgacct ggtagacgtc gtcgcgcgcc tgccacttgg gagcaacgcc cgcgaggatc
      301 tcgctcgggg cggtcatcgc gttcatggtg cgcgcgctga tcccgcgacg gtcgctctga
      361 tgccggacga tcccgaaatc cccgagcttc aagctccggc cgtcgcagac gaacacgttc
      421 atcggcgtga ggtcgcggtg gagcatctgg ccgcggtgga gctttccgag aacctggagg
      481 atgccggcga tctcccgacg cgcggtggtc tccggccacc ccttgccggt gcggtgaagg
      541 aacgcgcgca ggtctcccag cggcgcgtac tcgagggcga ggcagtacag gacatgccca
      601 tccgactgga tcagcggaaa cgcgtcgtag acccggattg cccgcggatg cccgtcgagg
      661 agttgaccga agtaggcctc gcgcaaccac ccgtcgattc gcgcgctcgc cttgatgcag
      721 acgatccgcg gaacagaccg ggagcgcccg agccggctgg cgagataagc ctgaccgaag
      781 cctccctcgc cgagcaggcg atcgatgcgg tactcgaggc gggtctccgg gctcgcgatg
      841 acctcctgcg gcatcaacag gtgttccgct cgcgcgaggg ccgcccggac ggcgcggaag
      901 gacatcggcc gggtcgtcgc ggcggtcgtc ggtttcatcg ccctcggggg tgatcgtcca
      961 tgagcgcgtt gatctcggcg tagacctccg cgccctcggt cagcggtccg gggtctcccg
     1021 tgcggagaaa cgcagcggct cgcgaagccg cctgcgcgaa tgccgccgac gcgatccacg
     1081 agcccgcgga gaggcggcgg acgccgagag cctggagctc cgacgcgggt ggcagaccgg
     1141 gccacacgag gacgttcagc ggcagccgcg cggccgcggc gatcgcgcgg atctcggcgg
     1201 catccgtcaa tgccggcacg aaaatgccgt cggcgcccgc ggcgcggtac cgctcggcgc
     1261 gggccaccgt ctcgtcgagc cgccgctccg gcggcgcaat tttgcgcagg tagacgtcca
     1321 cgcgggcgtt gacgaacacg tccacgccga gccgcacgcc gcttctcttc gcacgctcga
     1381 tcttggcgca cagaagttcc gggtcgccgc ctccgtcctc gacgttgatt cccacggccc
     1441 cggcttcgat cacgcgcgcg acagcgtcgc cgacggcgat cggatcgtcc gaatagccgc
     1501 cttccacgtc cgccgtgatc ggcacgttca ccacgcgcgc gatctccgcg accgtcgccg
     1561 cgagcagcgg caccggaagg aggtttccgt ccctgtaacc gtgcgaccgg gcgacgccgg
     1621 cactcgtggt ggcgatcgct ttcgcgccga ggctctcgat caggtgcgcg ctcccggcgt
     1681 cccacgcgtt ggcgagcagc agcagtcctt cctgatgaag ctcccgaaag acgcgtgttg
     1741 cgtcccgtcg ttcagaagtc atcgttcatc ctcttatgtg catggctcgc tcccgaggac
     1801 cgcactctaa ttcgatggct gcacggaatc cgacagggtg tcgattcctc gacagagccc
     1861 tccgccgcgc tcctccttct tccctcggac cgcgcagacg gaactcctgc gcttcaagcg
     1921 ctttggggac ctcgcgattc ccgcgccgca cacggcgagg cctggcacct ccttggctat
     1981 cagtcgaagc gtgtttccga cgtagaccca ctcggatatc cgacccccac ggattctcgc
     2041 tgcgagacgt agaggaggtg ttccggtcgg tttcaaccaa ggaggtcccg atggagacga
     2101 tgaaggcggt tcgagttcat cagtatggcg gcccggaggt tctcaagtac gaagaggcgc
     2161 cgcggcccca gcccggtccg ggcgaggttc tcgtgcgaat ccgcgcggtc ggcgtcaacc
     2221 cggtggactg gaaggtccgc gagggataca tgagggaagc cctcgcttat cggatgccct
     2281 tcgtgccggg gtgggacgtc tcgggcgtcg tcgaaaccat cggccccaac gtcacgctgc
     2341 tcgagaaagg ggacgaggtc tacgggtatc cctcgatcgt gcgcaacggg gcttacgccg
     2401 aatatgccgt cgtcccggag gcggagctcg cgctgaagcc ccgatctgtc gaccacgtcc
     2461 acgccgcgtc ggttccgatc gccgccctga ccgcatggca gggattgttc gacgtgggcg
     2521 ggctgcgcac ggagcagaag gtgctcatcc acggcggctc cggcggcgtc ggcagcttcg
     2581 cagtgcagct cgcgaggtgg aagggcgctt tcatcatcgc gacggcgtcc gggcgcaacc
     2641 aggagttcct ccgcaacctc ggcgcggacc tgacgatcga ctacgaaaag ggccgcttcg
     2701 accacgtggt ttgcgacgcc gacgtcgtct tcgacacggt cgggggagac acgctgaggc
     2761 gttcctggaa ggtgctgaag aagggcggca tccttgtgtc cacggtcgag gagccttctc
     2821 acgaggacgc ggcggctcac ggcgttcgcg gggcggtcgt cgacacgcac gcggatcgcg
     2881 gcgagctcgc cgaaatcgcg aagctggtcg acgccggaat ggtgcgaccg atcgtggagg
     2941 ccgtcttccc gctgaacgaa gcgcggcacg cccaggagct ctcccagacc ggccacgccc
     3001 gcggaaagat cgtgctgcag gtggcctgag gacgggggag gg
//
LOCUS       DASWAV010000632         3347 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U2665
            k141_755853, whole genome shotgun sequence.
ACCESSION   DASWAV010000632 DASWAV010000000
VERSION     DASWAV010000632.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960316
            Sequence Read Archive: SRR10260007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 3347)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3347)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:47:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,929
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3347
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_755853"
                     /isolate="SMAG_U2665"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.36444 N 132.31889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..825)
                     /locus_tag="VF958_13080"
     CDS             complement(<1..825)
                     /locus_tag="VF958_13080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014456830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfide/dihydroorotate dehydrogenase-like
                     FAD/NAD-binding protein"
                     /protein_id="HEX9150089.1"
                     /translation="MFKIVIKQSLGENVTLYEVEAPRVAKRHRAGQFVMVRPHGHSER
                     IPLTIADVDQARGTITLVVQAVGKTTTEMAEAFVQGDSFSNVAGPLGNPTHITIPGKE
                     QDFRGTVACVAGGIGAAPMLPIARAFRDAGHEVVTILGARRKDLLVFEDRLGAASTEL
                     MICTDDGSYGRKGFVTDALKDAIETRRASGGDVHLAIAIGPPLMMKFCSKTTQPYAVP
                     TLVSLNTIMVDGTGMCGGCRVMVDGAAKYVCVDGPEFDGHRVDFDGMMRRQAMYKPE"
     gene            complement(951..1136)
                     /locus_tag="VF958_13085"
     CDS             complement(951..1136)
                     /locus_tag="VF958_13085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9150090.1"
                     /translation="MTVAERSPRIPVTGFAAPAARAPLGPFTFERPAPGPRDVLIDIL
                     YCGVCHSVHQAHDDWEA"
     gene            complement(1147..1710)
                     /locus_tag="VF958_13090"
     CDS             complement(1147..1710)
                     /locus_tag="VF958_13090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9150091.1"
                     /translation="MRRFGLTVFSLLITTNIAAAQTPARRPAVSPPDAASVDAIVSAL
                     YGSVSHPSDAPPDFDRLRRIFLYVGMLVPPKTPEAADFTVMDVDQFSERYEKSVAARK
                     AKGEPPLGFFEREVSRRADCFGNICQIFSTYESRHAPADPTPFQRGINSIQLLRDGNR
                     WWIASVVWDAERPDNPVPAQYLPSPKS"
     gene            1770..>3347
                     /locus_tag="VF958_13095"
     CDS             1770..>3347
                     /locus_tag="VF958_13095"
                     /inference="COORDINATES: protein motif:HMM:TIGR01494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase
                     metal-binding domain-containing protein"
                     /protein_id="HEX9150092.1"
                     /translation="MSSPRRSESSESCFHCGLPVPVGPRWRAAVLGEVRDFCCGGCRA
                     VAEAISRGGLDDYYRLRTANPPTALDRGDAEERLFDREELQESFVRTLGGLRESSLVL
                     EGVRCPACLWLIEQHLRSQPGITEVTVAYASQTARVVWDPALAKLSAIRGAVREIGYE
                     AQPFDPAHRAHLESEAARRGSARLVFAGAVGMMVMNLAIAAYVKGGPDAAGKLPLWET
                     FARWSQLVGATVLLAYPGQDFFAGAWRDWKRRRVGMDVPIVLGLVVAWVGGAWATARG
                     KGPVYFDAVAMLVFFVLLARAFETRARLRAAAVLDRFAVVRPATASRRDEDGLESTVS
                     ALELVPGDVVRVRPGEIVPADGILLEGESGFDEAVLTGEPWPRTRRTGDAILAGSCNR
                     DQPVLLRVTNAGTASTLGEIRRLLERGLASRPPFAELADRLAGPLVAAVLAVSAATAV
                     FWAVRNPSIALPATVAVLMVTCPCALALATPIALAVAAGRFASIGVLPARMAAIERLA
                     IADTVAFDKTGTLTLPAL"
BASE COUNT          480 a         1118 c         1160 g          589 t
ORIGIN      
        1 ctccggcttg tacatcgcct ggcggcgcat catgccgtcg aagtcgaccc ggtgtccgtc
       61 gaactcggga ccgtcgacgc acacgtactt cgcggcgccg tcgaccataa cgcggcagcc
      121 gccgcacatc ccggtgccat cgaccatgat cgtgttgagc gaaacgagcg tcggaacggc
      181 gtaaggctgc gtcgtcttcg agcagaactt catcatcagc ggcgggccga tcgcgattgc
      241 caggtggacg tcgcccccgc tcgcgcgacg ggtctcgatc gcgtccttca acgcgtcggt
      301 cacgaatccc ttccggccgt aagagccgtc gtcggtgcag atcatgagct cggtggaggc
      361 ggcgccgagg cgatcctcga agacgagcag gtccttgcgg cgcgcaccga ggatcgtcac
      421 cacttcgtgg ccggcgtccc ggaaggcgcg cgcgatcggg agcatgggag cggccccgat
      481 gccccccgcg acgcaggcga ccgtgccccg gaaatcctgc tcctttccgg gaatcgtgat
      541 gtgcgtcggg ttgccgaggg gtcccgccac gttggaaaaa ctgtcgccct gtacgaacgc
      601 ctccgccatc tcggtggtgg tctttccgac ggcctggacg acgagggtga tcgtgcccct
      661 cgcctgatcg acgtccgcga tggtcagcgg gatccgctcg gagtgcccgt gcggtcgaac
      721 catcacgaac tggccggcgc ggtggcgctt cgccacccgc ggcgcttcca cctcgtagag
      781 ggtgacgttt tccccaagtg attgcttgat aacgatcttg aacacgctgt tttccccgcg
      841 gtgatggtgg gttccggagc ttcaaacccg tagatgcaat tgcatcagtg gtgccaggct
      901 ctcatccagc tgatgcaggc gcaggagaaa ggtctctttt caggcctttc tcaagcttcc
      961 caatcgtcgt gcgcctgatg gacgctgtgg cagacgccgc agtagaggat gtcgatcaga
     1021 acgtcccgcg gaccgggcgc gggacgctca aaggtgaagg gcccgagggg cgcccgggcg
     1081 gccggggcgg caaagcccgt gaccgggatc cggggggatc gttccgcgac ggtcattgtg
     1141 ctctcctcac gacttcggcg acggcaggta ctgagcgggg accgggttgt ccgggcgctc
     1201 cgcgtcccag acgacggacg cgatccacca gcggttgccg tcgcgcagca gctggatcga
     1261 gttgatgccg cgctggaacg gcgtcgggtc ggccggggcg tgccgcgatt cgtacgtcga
     1321 gaagatctgg cagatgttgc cgaagcagtc ggcgcgcctc gagacctcgc gctcgaagaa
     1381 tccgagaggc ggctcgccct tcgcttttcg cgctgcgacg gatttctcgt agcgctccga
     1441 gaactgatcg acgtccatca ccgtgaaatc cgccgcctcc ggcgttttcg gcgggacgag
     1501 catcccgacg tagaggaaga tccggcgcag ccggtcgaag tcgggaggcg catcggacgg
     1561 gtggctgacc gagccgtaga gagccgacac gatggcgtcg accgaggcgg cgtccggcgg
     1621 ggaaacggcc ggccttcgcg ccggggtttg cgcggccgcg atgttcgtcg tgatcagcag
     1681 cgaaaagacc gtgagcccaa atctccgcat cggttcctcc acgttcttct cgacgctacc
     1741 acgcggtccg acggagaata cgagctcact tgtcgtctcc ccgtcgcagc gaatcatcgg
     1801 agtcctgctt ccactgcggc ctgccggtgc cggtcgggcc ccgctggcgg gcggcggttc
     1861 tcggggaagt ccgtgacttc tgctgcgggg gatgccgcgc ggtcgccgaa gcgatctctc
     1921 gcggcggact cgacgactat taccggctcc ggacggcgaa cccgccgact gcgctcgacc
     1981 gcggcgacgc ggaagagcgc ctcttcgacc gcgaggagct gcaggagtcg ttcgtccgca
     2041 cgctcggtgg ccttcgggag tcgtcgctcg ttctcgaagg cgtccgctgt cccgcatgtc
     2101 tctggttgat cgagcagcat ctccgttcgc aaccggggat caccgaggtg accgtggcgt
     2161 atgcgagcca gacggcgcgc gtcgtctggg atccggcgct cgcgaagctc tccgcgatcc
     2221 ggggagccgt gcgcgaaatc ggttacgaag cgcaaccctt cgatccggcg caccgcgcgc
     2281 acctcgagtc ggaggccgcg cgccgcggat cggctcgcct cgtgttcgcc ggcgctgtcg
     2341 ggatgatggt gatgaatctc gcgatcgccg cttacgtcaa gggaggtccc gacgccgccg
     2401 ggaaattgcc tctctgggag actttcgccc ggtggtccca gctggtcggc gcgacggtcc
     2461 tgctcgctta tccgggtcag gacttcttcg ccggcgcctg gcgcgactgg aagcggcggc
     2521 gcgtgggtat ggacgttccg atcgtgctcg gtctcgtcgt cgcgtgggtc ggcggcgcct
     2581 gggcaaccgc tcgcgggaaa ggaccggtct atttcgacgc ggtcgcgatg ctcgtcttct
     2641 tcgtccttct tgctcgcgcc ttcgagacgc gcgctcgcct tcgcgcggca gcggtcctcg
     2701 accggttcgc cgtcgtccgt ccggcgaccg cgagccgcag ggacgaggac ggtctcgaat
     2761 cgaccgtctc cgctctcgag ctcgtgcccg gcgacgtcgt tcgggtccgt ccgggagaaa
     2821 tcgtccccgc ggacggcatc cttctcgaag gcgagtcggg cttcgacgag gccgtgctga
     2881 cgggcgagcc gtggccccgc acccgccgga ccggcgacgc gatcctggcg ggaagctgca
     2941 atcgggatca gccggtcctt ctccgagtga cgaacgcggg gacagcctcg acactcggcg
     3001 agatccggcg actgctcgag agaggcctcg cgagccgacc tcccttcgca gagcttgcgg
     3061 accggctggc cgggccgctc gtcgcggccg ttctggcggt ctcggcggcg actgcggtct
     3121 tctgggccgt gcgaaatccc tcgatcgctc ttccggcgac agtggcggtc ctgatggtca
     3181 catgcccctg cgcgctcgcg ctcgccacgc cgatcgcgct ggcggtcgcg gccggacgct
     3241 tcgcttcgat tggcgtgctc ccggcgcgga tggcggcgat cgagcggctc gcgatcgccg
     3301 acaccgtcgc gttcgacaag accgggactc tgacgctgcc ggcgctc
//
LOCUS       DASWAV010000633         2352 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U2665
            k141_717867, whole genome shotgun sequence.
ACCESSION   DASWAV010000633 DASWAV010000000
VERSION     DASWAV010000633.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960316
            Sequence Read Archive: SRR10260007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2352)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2352)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:47:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,929
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2352
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_717867"
                     /isolate="SMAG_U2665"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.36444 N 132.31889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..556)
                     /locus_tag="VF958_13100"
     CDS             complement(<1..556)
                     /locus_tag="VF958_13100"
                     /EC_number="5.1.99.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate epimerase"
                     /protein_id="HEX9150093.1"
                     /translation="MIPILDSRQMRSADESAIRAGTPSAELMERAAGGVADAVRKQGV
                     GWRSVVVVCGPGNNGGDGLAAARMLSESFAVRVFTLGDAAAYRGDAEANARRAREAGL
                     RLESLSPSGGLEVLSRALGESDAVVDALFGTGLDRPLDGPALAAVEAINRAGRPVVSA
                     DVPSGLSSDTGELIGGSICANVTVA"
     gene            complement(553..1251)
                     /locus_tag="VF958_13105"
     CDS             complement(553..1251)
                     /locus_tag="VF958_13105"
                     /inference="COORDINATES: protein motif:HMM:TIGR04282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04282 family arsenosugar biosynthesis
                     glycosyltransferase"
                     /protein_id="HEX9150094.1"
                     /translation="MVANPSTLVLFARAPIAGEVKTRLAAALGPAGAARLYRAFLEDA
                     SRVYVGPRWTPVLYADLDPDAPSLVSLFGAPWRREAQAPGNLGRRLTEAFRTEFSRGA
                     SAVLAVGSDHPALARRSLNELLEAVSRGSDASVIPARDGGYCAIALSNRVEPEAAFDG
                     IPWSSPDTFAKTLGRMRERGLSVAVLDPAYDVDRPEDLELLREDLARRDPEGNDFPRS
                     TAAALADLFPELAG"
     gene            complement(1309..>2352)
                     /locus_tag="VF958_13110"
     CDS             complement(1309..>2352)
                     /locus_tag="VF958_13110"
                     /inference="COORDINATES: protein motif:HMM:TIGR03436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HEX9150095.1"
                     /translation="SYSQSLRNDLEFTVDAFKTSLNALAGVEGRKIFIYVSEGLPSSA
                     GAELFDTIQKKFPGGRGTLETLDFDMNTRYASLVQAANANGVTIWALDASGLAADELV
                     SAENRYMESRPSSFLMRQNTQAPLLMLAEQTGGMAAVNTNDWKESLDELSKDFSNFYS
                     LGYRTTRGASDRPHAVEVKVKRKGLRVRSRKGLLEKSTETRTAEAVVASLFYPRDENP
                     LGVSVTLGEARPYDSENFLLPVRIAVPVGKLGLVPSGDRYEGQFFVYFVVLDALGKQS
                     DLQVQRQAVSVPAKDFTTAQKKDFYYDVQLLVVPGGQKLSVALRDGVSNLTSYLQKNV
                     FVSVLPKETKKTG"
BASE COUNT          402 a          784 c          815 g          351 t
ORIGIN      
        1 aggcgaccgt gacgttcgcg cagatcgatc cgccgatcag ttctcccgtg tccgaagaga
       61 gcccggatgg aacgtcggcg gagacgacgg gtcggcccgc gcggttgatc gcctccacgg
      121 cggcgagcgc cggaccgtcc aggggccggt cgaggccagt gccgaagagc gcgtccacca
      181 cggcgtcgga ttcgccgagc gcacgagaga gaacctcgag tccgcccgag ggggacaggc
      241 tctcgagacg caagcccgct tcgcgcgcgc gccgcgcgtt ggcctcggcg tccccgcgat
      301 acgccgccgc atcgccgagc gtgaacacgc ggacggcgaa gctctccgag agcatccggg
      361 cggcggcgag tccgtcgccg ccgttgttcc cggggccgca gacgacgacg acggaccgcc
      421 atccgacgcc ctgctttcgc accgcgtcgg cgactccgcc cgccgctctc tccatgagtt
      481 cagccgacgg agttccggcg cggatcgcgg attcgtccgc cgagcgcatc tggcgggaat
      541 cgaggatcgg gatcatccgg cgagctccgg aaagagatcc gcgagggctg cggcggtcga
      601 ccgcggaaag tcgttccctt cgggatcgcg tcgggcgaga tcctcgcgca gcaactcgag
      661 gtcctcgggg cgatcaacgt cgtaagccgg gtcgaggacg gcgaccgaaa gcccccgctc
      721 cctcattcgc ccgagcgtct tcgcgaaggt gtccggcgaa gaccacggga tcccgtcgaa
      781 cgccgcttcc ggctcgacgc gattcgaaag ggcaatcgcg cagtagccgc cgtctcgggc
      841 cgggatcacg gaggcatcgg agccccggga gaccgcctcg aggagctcgt tcagggagcg
      901 ccgggcgagg gcgggatgat cggatccgac agcgagcacg gccgacgcgc cgcgggagaa
      961 ctcggtgcgg aacgcttccg tgagacgccg gcccaggttc cccggcgcct gcgcctcacg
     1021 gcgccacggc gcgccgaaga gggagacgag cgacggcgcg tccggatcga gatcggcgta
     1081 gagcaccggt gtccagcggg gaccgacgta gacgcgcgac gcatcttcga gaaaggcgcg
     1141 gtagagccgc gcggccccgg cggggccgag cgccgccgcg agccgcgtct tgacctcccc
     1201 ggcgatgggc gctcgcgcga acagcacgag tgtcgatggg ttcgcgacca cccgccgatt
     1261 atgtcctcgc tcaatcgccg ggcccgggcc agcggcgaga acccgcgcct accccgtctt
     1321 cttggtctcc ttgggcagca ccgagacgaa gacgttcttc tgaaggtagg acgtcagatt
     1381 cgaaacgccg tccctcaggg cgacggagag cttctggccc cccggaacga cgaggagctg
     1441 tacgtcgtag tagaagtcct tcttctgtgc ggtcgtgaag tccttcgcgg gaacgctcac
     1501 cgcctgccgc tgaacctgaa ggtccgactg cttgccgagc gcgtcgagca cgacgaaata
     1561 cacgaagaac tgtccttcgt accgatcgcc ggacgggacg aggccgagct tcccgacggg
     1621 aacggcgatt cgaaccggaa gcaggaagtt ctccgagtcg tacggacgcg cctcgccgag
     1681 agtcacgctg acgccgagcg ggttctcgtc ccggggatag aagaggctcg cgaccaccgc
     1741 ctccgcggtg cgcgtttcgg tcgacttctc gagcagaccc ttgcgagatc tcacgcgaag
     1801 acccttgcgc ttcaccttca cttcgaccgc gtggggccgg tcggaagcgc cacgagtcgt
     1861 tcggtagccg agcgaataga aattcgagaa gtccttcgag agctcgtcca ggctctcctt
     1921 ccagtcgttc gtgttgaccg ccgccatgcc accggtctgc tccgcgagca tgagaagcgg
     1981 cgcctgggtg ttctggcgca tcaagaagct cgaaggccgg gattccatgt accggttctc
     2041 cgcagagacg agctcgtcgg ccgccagccc cgacgcgtcg agcgcccaga tcgtcacccc
     2101 gttcgcgttg gcggcctgga cgagggaggc gtagcgggtg ttcatgtcga agtcgagcgt
     2161 ctcgagagtg ccgcgcccgc cgggaaattt cttctggatc gtgtcgaaga gctccgctcc
     2221 tgctgaggag ggaaggccct cggagacgta gatgaagatc ttgcgcccct cgacccccgc
     2281 gagggcgttc aaggacgtct tgaaagcgtc gacggtgaac tcgagatcgt tgcgcagcga
     2341 ctgggagtag ga
//
LOCUS       DASWAV010000634         3225 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U2665
            k141_237509, whole genome shotgun sequence.
ACCESSION   DASWAV010000634 DASWAV010000000
VERSION     DASWAV010000634.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960316
            Sequence Read Archive: SRR10260007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 3225)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3225)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:47:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,929
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3225
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_237509"
                     /isolate="SMAG_U2665"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.36444 N 132.31889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(13..1293)
                     /locus_tag="VF958_13115"
     CDS             complement(13..1293)
                     /locus_tag="VF958_13115"
                     /inference="COORDINATES: protein motif:HMM:NF024110.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX9150096.1"
                     /translation="MIFAVARTHFAKLKRDRAAFVLAFVVPVVFFSVFASIFGGSSSR
                     AATRQIPVAVVDEDSTANSRRFVDALKREKGLRVLDVKTLPGGRTSPIPDAATAEKMV
                     REGDVPIALIVPKGFGAAPIRFGKSGGAPALSILADTSDPIAPQVLSGLLQKVAMTSM
                     PDVLARSGIEELDRWGGELTPEQRARLERNVESLRSESGKSGEAGAGRDGGLVPVEIR
                     DVLGEKKKNPTVAVFAAGLGVMFLLFSAAGAGGALIEEVESGTLDRILSTRVTMTQLL
                     LGKLLYLSCVAVAQLAVMFVWGEIFFGLELHRHIPGFIVMAVVTALASSAFGLVLAAI
                     ARTRMQLVALSNLLILVMSALGGSMFPRYLLSETVQKIGLVTLNAWAIDGFMKVFWRD
                     EPLSALAPQVAFLVGAALVLFALARRLARRWETT"
     gene            complement(1290..2222)
                     /locus_tag="VF958_13120"
     CDS             complement(1290..2222)
                     /locus_tag="VF958_13120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009154354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX9150097.1"
                     /translation="MPDALPLEIRDVVKSFGERRALDGVSLEMRAGEILGLLGPNGAG
                     KTTLVRSVVGRVRPQSGTVRVLGRPPDAPESKANVGYVTQEISLYPLLSPEENLEAFG
                     RYQGLRGEALAKAVEDSLRWTGLADREKEKTERLSGGMKRRLNIAAGTIHGPRLLLLD
                     EPTVGVDPQSRERIYGMIEELRSRGVSILYTTHYMEEAERLCDRIAIIDHGRVIALGT
                     KDELVRSTLPAARMLTIDMEMPPTDALRNELEGRGASITNSTVHVPVTEPAREIPDLL
                     TMFRNDGVRIRDLTLKTATLESVFLHLTGRELRE"
     gene            complement(2358..>3225)
                     /locus_tag="VF958_13125"
     CDS             complement(2358..>3225)
                     /locus_tag="VF958_13125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012632950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9150098.1"
                     /translation="AGYPSKTGEDLLTAVDAWRRERMVPGKSIPMLADALIAQFSAGT
                     AKYLAPHLPQDLRGVPLANMKFLPIKDAWFSGSMNYIGRARRPDGSPEYEATYEINAS
                     LQISVPEFAQLVSHEVVPGHVTTSAVIQGLYVRKRLGFEATVLTMNSRGAALAEGIAN
                     NAILIAHGVTEVEALPGDDMQLGVLLALLQDDAKNQSSWLTWKEGVAQKDVAATLRRE
                     YLVSEERADKLSGAWGRHPLLGRMYLPCYRAGTEAVADLRRRYPSEKVLPALYGCRGM
                     VDVVTVRRAVET"
BASE COUNT          545 a         1065 c         1107 g          508 t
ORIGIN      
        1 cccgcacgcg cgtcaggtgg tttcccaccg ccgcgcgaga cgccgcgcga gagcgaagag
       61 aacgagggcc gccccgacga ggaacgcaac ctgcggtgcg agggccgaga gcggctcgtc
      121 gcgccagaaa accttcatga atccgtcgat cgcccaggcg ttcaacgtca cgaggccgat
      181 cttctggacg gtctccgaga ggaggtaccg cgggaacatg ctgcccccga gcgccgacat
      241 cacgaggatc aggaggttcg agagcgcgac gagctgcatc cgggtccggg cgatcgccgc
      301 gagaacgagc ccgaatgcgg acgacgcgag cgccgtaacc acggccatca cgatgaatcc
      361 ggggatgtgc cggtgaagct ccagcccgaa gaagatttct ccccagacga acatcaccgc
      421 cagctgcgcg acagcgacgc acgagagata gaggagcttt ccgagcagga gctgcgtcat
      481 cgtgacgcgg gtcgagagaa tccggtcgag cgttccgctc tcgacctcct cgatcagagc
      541 ccctccagcg cccgcggcgg agaagaggag aaacatcacg ccgagcccgg cagcgaagac
      601 cgcaaccgtc gggttcttct tcttctcgcc gagcacgtca cggatctcga ccgggacgag
      661 cccgccgtct cgaccggctc cggcctcgcc gctcttgccg ctctccgagc gcagcgactc
      721 gacgttgcgc tcgagccgcg cccgctgctc gggcgtcagc tcgccgcccc accggtcgag
      781 ctcttcgatt ccggaccgcg ccaggacgtc gggcatcgac gtcatcgcga ccttctgaag
      841 gagtcccgaa aggacctgcg gagcgatcgg gtccgacgtg tcggcgagga tcgagagcgc
      901 cggcgcgccg cccgacttcc cgaatcggat cggcgccgcg ccgaacccct tcggcacgat
      961 cagggcgatc gggacatccc cctcgcggac catcttctcg gccgtcgccg cgtcggggat
     1021 cggagaggtc cgtccgcccg ggagcgtctt gacgtcgagg actcggagac ccttctctct
     1081 cttcaacgcg tcgacgaacc gccgggagtt cgcggtcgag tcttcatcga cgaccgcgac
     1141 cgggatctgc cgggtcgcgg cgcgcgagga gctgccgccg aagatgctcg cgaagacgga
     1201 aaagaagacg actggcacga cgaaggccag cacgaacgcc gcgcggtcgc gcttcagctt
     1261 cgcgaagtgg gtgcgagcga cggcgaagat cattcgcgca gttcccgtcc ggtcaggtgg
     1321 aggaacaccg actcgagggt cgccgtcttc agagtcaggt cgcggatgcg gacgccgtcg
     1381 ttgcggaaca tcgtcagcag atcggggatc tcgcgcgccg gctcggtcac cggaacgtgg
     1441 acggtcgagt tggtgatcga ggctccacgg ccctcgagct cattgcgcag ggcatccgtc
     1501 ggcggcatct ccatgtcgat cgtcagcatc cgcgcggccg gcagcgtcga gcgcacgagc
     1561 tcgtccttcg ttccgagcgc gatcacccgc ccgtggtcga tgatcgcgat gcggtcgcag
     1621 agccgctccg cttcctccat gtagtgcgtc gtgtagagga tcgagacacc gcggctgcgc
     1681 agctcctcga tcatcccgta gatgcgctcg cgggactgcg ggtcgacgcc gaccgtcggc
     1741 tcgtccagca ggagcaggcg gggtccgtga atcgttcccg ccgcgatgtt caagcgccgt
     1801 ttcataccgc cggacaggcg ctcggtcttc tccttttctc ggtccgcgag cccggtccag
     1861 cgcaggctgt cctcgaccgc cttcgcgagc gcttccccgc gaaggccctg ataccggccg
     1921 aatgcctcga ggttctcctc gggggagagg agcggataga gagagatctc ctgggtgacg
     1981 taaccgacgt ttgccttgga ttcgggggca tcgggaggtc gtccgagcac gcgcaccgtt
     2041 cccgactgcg gccggacgcg gccgacgacc gaacggacga gggtcgtctt gccggcgccg
     2101 ttgggtccga ggagtccgag gatctcgccg gctcgcatct cgagactgac gccgtccaat
     2161 gcgcggcgtt cgccaaacga cttgacgacg tcgcggattt cgagcgggag tgcgtccggc
     2221 acgttggaag ggagtctatc gtcgcctctc acgcggcgcg ctgtgcgcat cgtcagcagc
     2281 acggcgacga cgatcggagg aagaagtttt ggcgacgtcc ggactctgct gcaccccgag
     2341 ctgtctctgg gactccctca ggtctcgacg gcccggcgga ctgtgacgac gtccaccatg
     2401 ccgcggcagc cgtagagagc gggaaggacc ttctcggagg ggtagcggcg gcgcaggtcg
     2461 gcgacggcct cggtgccggc gcggtagcac ggcaggtaca tgcgtcccag aagcgggtgg
     2521 cggccccagg cgccggagag cttgtcggcg cgctcttcgg agacgagata ctcgcgacgc
     2581 agcgtcgccg cgacgtcctt ctgggccacg ccttccttcc aggtgagcca ggaagactgg
     2641 ttcttggcgt cgtcctgaag gagagcgagg agcacgccca actgcatgtc gtcgccgggg
     2701 agtgcttcga cttcggtcac gccgtgtgcg atcaggatcg cgttgttggc gatcccttcc
     2761 gccaacgctg cgccgcgcga gttcatcgtc aggacggtcg cttcgaatcc gagacgcttg
     2821 cgcacataaa gaccctggat gaccgctgaa gtcgtgacgt ggccgggcac gacctcgtgg
     2881 gagacgagct gcgcgaactc cggaacggag atctgcagcg acgcgttgat ctcgtacgtt
     2941 gcctcgtact cgggagagcc gtcgggcctg cgggcgcggc cgatgtaatt catggaaccg
     3001 gagaaccagg cgtccttgat cggcaggaac ttcatgttgg cgagcgggac accgcgcagg
     3061 tcctgcggca gatgcggcgc gaggtacttc gccgtgccgg cggagaactg agcgatcaga
     3121 gcgtcggcga gcatcgggat ggacttgccg ggcaccatgc gctcgcgccg ccaggcgtcc
     3181 acggcagtca gaagatcctc gcccgtcttc gacggatagc cggcc
//
LOCUS       DASWAV010000635         7543 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U2665
            k141_199610, whole genome shotgun sequence.
ACCESSION   DASWAV010000635 DASWAV010000000
VERSION     DASWAV010000635.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960316
            Sequence Read Archive: SRR10260007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 7543)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7543)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:47:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,929
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7543
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_199610"
                     /isolate="SMAG_U2665"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.36444 N 132.31889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(179..331)
                     /locus_tag="VF958_13130"
     CDS             complement(179..331)
                     /locus_tag="VF958_13130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9150099.1"
                     /translation="MHRLSVRSEESPRAPTAARYLVVGNASGVVAYTPSGVVVGMNSA
                     EKGVTR"
     gene            complement(550..1068)
                     /locus_tag="VF958_13135"
     CDS             complement(550..1068)
                     /locus_tag="VF958_13135"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HEX9150100.1"
                     /translation="MAFPSDEFLMAAFTKGDRESFDRLASRYSSLLDTYFRRRLPDDG
                     RAEELRQETLLAILSLAPSYREKGRFRSLLFSVAYRKLVSARRAQKPAERITKDVAGP
                     AEDLESLSVRTALRALPEALREALMLTYFEGLTSREAGEVLECSEDAVRARVCRARSA
                     LARRLNEKRRPG"
     gene            complement(1068..1463)
                     /locus_tag="VF958_13140"
     CDS             complement(1068..1463)
                     /locus_tag="VF958_13140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9150101.1"
                     /translation="MRRDLVEKRFDRPPPEVADEVGISRLAVGRELLDRRCRRDESGA
                     PKLSQEELRRRRESRRALDAVETGELPRGLEGRLAVDLSVDGCRNRVGPEGDVAILCG
                     GGRGRRREQRESCAPVTFQRSDAITLADE"
     gene            1524..3107
                     /locus_tag="VF958_13145"
     CDS             1524..3107
                     /locus_tag="VF958_13145"
                     /inference="COORDINATES: protein motif:HMM:NF016289.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4910 domain-containing protein"
                     /protein_id="HEX9150102.1"
                     /translation="MDATTELVDVGGGTEEKDYSGKEVRGKIVLADGALPAVHKLAIF
                     GKGASGILSDYPNQTTAWSGDDRDLVRWGHLSPYETRNAFAFMVSKRQAEDYRRRLTA
                     GEVIVLRAHVLAEMVPATYDVVVATIEGTDRAAGEVVLTAHLCHESAGANDNASGSAV
                     LFEVARALKTAISHGSLPTPTRTIRFLWLPEIAGSQAYLVRNRELARRFVAGIHLDMV
                     GGLLATTHGTLHVSRSAGSLPHVVNDVAEAFLREILDASARYAERGGDPRRGFVWPAG
                     AREALLADLRGIELGSDHEVFEDSSFGVPMVYFHDWPDVTIHTNKDLPENLDATKLGR
                     VAYLSAGIAWTLAALPDSEAPRLIALAAAVAESRIAQAGFRPDWSRRDAALARREAVE
                     QGAATVRSVGEMWPAVARVARETERGIRGLAPAIPPESGGDRRVPVRSPEIVGPLDVY
                     YFNFLDRALGEGAASKIALAKRDGGDVLGWEAFNLADGRRSVSDIRDILSGRYSPVPG
                     SEISEYFDLLARAKAVSLK"
     gene            3107..4261
                     /locus_tag="VF958_13150"
     CDS             3107..4261
                     /locus_tag="VF958_13150"
                     /inference="COORDINATES: protein motif:HMM:NF012488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HEX9150103.1"
                     /translation="MAFDYSPENLDREFPVRRNLVYWNHAAVAPLPRRVADAIDAHTA
                     NVRDRGAADWRAWYRSIDETRAKAARLVGARASEIAFLPSTSWALNVVAQAYPWKSGD
                     NVVGDDMEFPANVYPWMLLEPRGVEYRIAHNRGGRIGVDEIAAVVDSRTRVVAVSWVA
                     FHNGWVFPIEEIGAFCREKGILFVVDAIQGLGALPLDVGEANVDVLAADSHKWLLGAE
                     ACAVFYVSEDARERVPTAFGGWWNVKHDSTHAGGYLDYRLDFHLGGRRYEPGSLPTAQ
                     IMGLAAVLDLFEEMEVEEISRRIVANVSALAGGLSERGWEVTTPEPLRSGILSAIPPG
                     ADVHGMAKQLESRGVIASPREGAVRFSPHAYNGAEEVERILAAADEIAKS"
     gene            4194..5177
                     /locus_tag="VF958_13155"
     CDS             4194..5177
                     /locus_tag="VF958_13155"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: protein motif:HMM:NF003915.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016835 - carbon-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0046348 - amino sugar catabolic process
                     [Evidence IEA];
                     GO_process: GO:0097173 - N-acetylmuramic acid catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HEX9150104.1"
                     /translation="MRTTGRKKSSGSSRPPTRSPRAEPRHRGTPWKRLSTEATHPASA
                     RLDRLGIERIVALMQAEDRRVLDALRKARPAIVRAAKLFQETYGSGGIALLFGAGTSG
                     RLAVLEAAELPPTFGTEPRRARAVIAGGRGAVFRAKEGAEDRSDEGSRAAAKARKGDL
                     AIGVSASSVTPFVRAALERARVEGARTVLVTASRSRDTTPLADVVVRLDVGPEVVAGS
                     TRLKAGTATKLALNQITTSALASAGKVYGPWMVDLRAGSAKLRDRALRIVAAACGVSP
                     EQARRLLGRSRGEVRTAIVMGRTGVGYADARSRLDAAGGDLRRVLEGSKPR"
     gene            5207..5638
                     /locus_tag="VF958_13160"
     CDS             5207..5638
                     /locus_tag="VF958_13160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9150105.1"
                     /translation="MLSFVHEHSLSLAAIAILATWTILYARSDPDTRWGEFFGNAVAD
                     WSGTLFLVLGTKLLYERGSVESRRAPRHFQNRTIDLLYHHSLTIFLVVTGAIWLFLFH
                     HEKPGSKWGQVYGNILSEWLQMLGIVFLTKRLLERGSKESH"
     gene            complement(5635..6360)
                     /locus_tag="VF958_13165"
     CDS             complement(5635..6360)
                     /locus_tag="VF958_13165"
                     /inference="COORDINATES: protein motif:HMM:NF013356.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HEX9150106.1"
                     /translation="MKVEVFATPEALARAAAERVGGILRRRAATVLLLPVGKTAVPVY
                     RELARLNAAGRASFRRATTFNLDELSVPPDDPRSFRSFMERHLFSKVDLDRRRIRFLR
                     GDAPDAERECARYERVLAREGPPDIAFVGVGINGHVAYLEPARALPPRTSLVSLSAST
                     RRGLAADGVRPVPRDAFTVGIETILSSRTILLVATGRKKAAAVAAALEGPVTPRCPAS
                     FLALHPGLSVLLDLPAGTRLRSG"
     gene            complement(6357..7112)
                     /locus_tag="VF958_13170"
     CDS             complement(6357..7112)
                     /locus_tag="VF958_13170"
                     /inference="COORDINATES: protein motif:HMM:NF016668.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ChbG/HpnK family deacetylase"
                     /protein_id="HEX9150107.1"
                     /translation="MKRLIVNADDFGRTPGVNAGVVEAHLLGIVTSATVMVLEPAAGE
                     GVADARRRAPRLSLGLHFALTGGGLPASAPESIRSLLVDGRFRRFPADFPEELAPEEV
                     ARELEAQISLFEKMVGDPPTHIDSHHHSALHPSVQPVVADAALRLGVPVRASSVAARD
                     ALRGRGVRTPDRFVDGFHGAGATAENLRSIIGGLAHETTELMCHPAHVDEGLLSGSSY
                     AGERERELALLCDPDVKAVLVEQGVGLVPFREL"
     gene            complement(7109..>7543)
                     /locus_tag="VF958_13175"
     CDS             complement(7109..>7543)
                     /locus_tag="VF958_13175"
                     /inference="COORDINATES: protein motif:HMM:NF013986.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BadF/BadG/BcrA/BcrD ATPase family protein"
                     /protein_id="HEX9150108.1"
                     /translation="IRDVLRGADGRGRATSLDRALSEHFSIAQPDQILYAIYDGEFPR
                     TQVAKFAPRVEEAARAGDAVAKAILSDAAGELTRAAASVRDRLRLEESAYDVVLSGGT
                     FAAVPTLADDVARRLASTNAHVSRLVEEPAMGAVKLAIEALG"
BASE COUNT         1196 a         2624 c         2587 g         1136 t
ORIGIN      
        1 ccttggacgt cgggatcgat cagaacgggc aggttcgcga gcgcgcccag ggtctgaacg
       61 acgtcggcga tcttcgcgtc cttcaggtcc agcgaaatcg gctccttcgg attaaaatcg
      121 gacgagcggg cgatcaaaga cgaggacaga aagagaccga gaagcatcat gcctcttctc
      181 accgggtcac ccctttctcc gcggagttca tgcccacgac gactccggat ggcgtgtagg
      241 cgacgacgcc cgacgcatta ccaacgacca gataccgagc cgccgtcggc gcccggggcg
      301 attcttccga tctgaccgac agccggtgca tgcccagaac gactatcccc aaaatggcgg
      361 ccgtggctcc gaatcgaacg acctcctctg cgatcgacct gcgggcggga tccaggcgta
      421 acagtgcccg ctcaacgaaa cggcgttccc ttcggcaggc cgcgcaggag ccggcgtgcc
      481 ggaggacttc cgtggcgtct tcgcgcgaat ctgtgaattc ctccaatcgc gacagatact
      541 cgtcgtgtgt catccgggtc tccgtttctc gttcaaccgg cgggcgaggg cgcttctcgc
      601 gcggcagacg cgagcccgaa cggcgtcttc cgagcactcc agcacttcac ccgcttccct
      661 cgaggtcagc ccctcgaaat aggtaagcat cagcgcttcc cgcaaggcct ccggcagcgc
      721 ccgcagagcg gtgcgcacgg agagactctc cagatcctcc gcggggcccg ccacgtcttt
      781 ggtgatccgc tccgcgggct tttgcgcacg tcgcgcggag acgagcttgc gataggcgac
      841 cgaaaagagc agcgatcgga atcggccctt ctcccggtag gacggggcga gcgagaggat
      901 cgcgaggagt gtctcttgtc gcagctcctc cgcccggccg tcgtccggga gccgccttcg
      961 aaaataagtg tccagaagag agctgtatcg cgacgccaga cgatcgaaag actcgcggtc
     1021 ccctttcgtg aaggcggcca tcaggaattc gtcggaaggg aacgccatca ttcgtctgct
     1081 agtgttatcg catcggaacg ctgaaacgtg acgggggcgc agctctcgcg ctgctcgcgg
     1141 cgacggccgc ggccgccgcc gcacaggatc gccacatccc cttctggccc gacgcgattc
     1201 cggcagccat ccacgctcag gtcgacggcc aggcggccct cgagaccgcg cgggagctct
     1261 cccgtttcca ccgcgtccag ggctctccgg gattcgcggc ggcggcggag ctcctcctgg
     1321 gaaagcttcg gcgcgccgga ttcatcacga cggcagcggc gatcgagaag ttcccggccg
     1381 acggccagac gcgatatgcc cacttcatca gctacctcgg gtggcggccg gtcgaagcgt
     1441 ttctcgacga ggtcgcgccg catccgggtc gcatcctttc gttcccggag ctcccggtcg
     1501 ccctcgccga ctacagccag aacatggacg ccacgaccga gctcgtcgac gtcggcggcg
     1561 ggacggaaga gaaggactat tccggcaagg aagtccgggg aaagatcgtc ctcgccgacg
     1621 gggcgctccc cgcggtccac aagctggcga tcttcggcaa gggggcgtcg gggattctct
     1681 ccgactaccc gaaccagacg accgcctggt ccggtgacga tcgggatctc gtgaggtggg
     1741 gacacctttc cccatatgag acccgcaacg cgttcgcgtt catggtgtcg aagcgtcagg
     1801 ccgaggacta ccgccggcgg ctcacggccg gcgaggtcat cgtcctccgg gcgcacgtcc
     1861 tcgcggaaat ggtcccggcc acgtacgacg tggtcgtcgc gacgatcgaa gggaccgacc
     1921 gcgcggccgg tgaggtcgtc ctgaccgcgc acctctgcca cgagtccgcc ggcgcaaacg
     1981 acaacgcctc gggcagcgcc gtcctcttcg aggtcgctcg cgcgttgaag acggcgatct
     2041 cgcacgggag cctgccgacg ccgacccgca cgatccgatt cctctggctg cccgagatcg
     2101 ccgggtctca ggcctatctg gtccgaaatc gcgagctcgc ccgccggttc gtggccggga
     2161 tccacctcga catggtcggc ggcctcctcg cgacgactca cggcacgctc cacgtttccc
     2221 gctccgcggg gtcgctcccg cacgtcgtca acgacgtggc cgaagcgttc cttcgggaga
     2281 tcctcgatgc ctcggcccgg tacgcggagc gcggaggaga tccgcgccgc ggattcgtgt
     2341 ggcccgccgg cgcgcgggag gcactcctcg cggacctgcg gggaatcgag ctcggcagcg
     2401 accacgaagt gttcgaggat tcgagcttcg gggtgccgat ggtctatttc cacgactggc
     2461 cggacgtcac gatccacacg aacaaggacc tgcccgaaaa cctcgacgcg acgaagctcg
     2521 gccgcgtcgc ctatctgtct gcgggaattg cctggacgct cgccgccctt cccgactccg
     2581 aggcgccgag gctgatcgcg ctcgctgccg ccgtggcgga atcccgtatc gcgcaggccg
     2641 ggtttcggcc cgactggtcg cgtcgggatg cggccctcgc gcgtcgcgaa gcggtcgagc
     2701 agggggccgc cacggtgcgg tccgtcggcg agatgtggcc agcggtcgcc cgagtggcgc
     2761 gagagacgga gcgaggcatc cgcggactcg ctccggcgat accgccggag agcggcggag
     2821 accgtcgggt cccggtccgc agcccggaga tcgtcggacc cctcgacgtc tactacttca
     2881 acttcctcga ccgggcgctc ggcgaaggcg cagcctcaaa gatcgcgctc gcgaagcgcg
     2941 acggcggcga cgtcctcgga tgggaagcat tcaacctcgc ggacgggcgg cgctcggtct
     3001 ccgacatccg cgacatcctg tcgggccgct actcccccgt gccgggctcc gagatctcgg
     3061 aatacttcga cctcctcgcg cgtgcgaagg cggtctcgct caagtagtgg cgttcgacta
     3121 ctcgcccgag aacctggacc gcgaattccc ggtccggcgc aacctcgtct actggaatca
     3181 cgccgctgtg gcgccgctcc cccgccgggt cgccgacgcg atcgacgcgc acaccgcgaa
     3241 cgtgcgcgac cggggcgcgg cggactggcg cgcgtggtat cggtcgatcg acgagacccg
     3301 cgcgaaggcc gcgcggctgg tgggcgctcg cgcgagcgag atcgccttcc tgccatcgac
     3361 gtcctgggcg ctgaacgtcg tcgcgcaggc atatccctgg aagagcggcg acaacgtcgt
     3421 cggcgacgac atggagttcc ccgcgaacgt gtatccctgg atgctcctcg agccgcgcgg
     3481 cgtcgagtac cggatcgcac acaatcgagg aggacggatc ggagtcgacg agatcgcggc
     3541 cgtcgtcgac tcccgcacgc gcgtcgtcgc cgtctcctgg gtcgcgttcc acaacgggtg
     3601 ggtgtttccg atcgaagaga tcggagcgtt ctgccgcgag aagggaatcc tcttcgtcgt
     3661 ggacgcgatc cagggtctgg gcgcgctgcc tctcgacgtc ggtgaggcga acgtcgacgt
     3721 gctcgccgcc gattcgcaca agtggctgct cggcgcggag gcgtgcgcgg tcttctacgt
     3781 ctcggaggac gcgcgagaga gggttcccac ggccttcggc ggatggtgga acgttaagca
     3841 cgactcgacg cacgccggtg gatacctcga ctacaggctc gactttcacc tcggcggtcg
     3901 ccggtacgag ccgggctcgc tcccgaccgc gcagatcatg gggctcgcgg ccgtgctcga
     3961 tctattcgag gagatggagg ttgaagagat ttcgcggcgg atcgtcgcga acgtgagcgc
     4021 gctcgccggc ggcctctcgg agcgaggctg ggaggtcacg acccccgagc ccctccgctc
     4081 cgggatcctc tcggcgattc cgcccggcgc cgacgttcac gggatggcga agcagttgga
     4141 gtcgcgcggc gtgatcgcct cgccgcggga gggcgcggta cgcttctccc cgcatgcgta
     4201 caacggggcg gaagaagtcg agcggatcct cgcggccgcc gacgagatcg ccaagagctg
     4261 agccgcggca tcgcgggact ccctggaagc gtctctcgac cgaagccacg cacccggcgt
     4321 ctgcgcgcct cgaccggctc gggatcgagc gcatcgtcgc gctcatgcag gccgaggacc
     4381 ggcgcgtgct cgacgcgctc cgcaaggcgc gccccgcgat cgtccgcgcg gcaaagcttt
     4441 tccaggagac ctatggctcg ggcggcatcg ccttgctctt cggcgcgggg acgagcgggc
     4501 gcctcgcggt cctggaggcc gccgagctcc cgcccacgtt cggaaccgag ccgcgccgcg
     4561 cccgcgccgt gatcgccgga gggcgcggag cggtcttccg ggcgaaggag ggcgctgagg
     4621 accgtagcga cgagggatcg cgcgcggccg cgaaggctcg gaagggtgat ctcgcgatcg
     4681 gcgtctcggc ctcctccgtc actcctttcg ttcgagcggc gctcgaacgg gcgcgtgtcg
     4741 aaggcgcccg gaccgtgctc gtcaccgcga gccgctcgcg cgacacaacg ccgctcgccg
     4801 acgtcgtcgt gcgcctcgac gtcggacccg aggtcgtcgc gggatcgacg cgactgaagg
     4861 ccggcaccgc cacgaagctc gcgctgaacc agatcacgac gtcggcgctc gcgtcggcgg
     4921 ggaaggtcta cgggccgtgg atggtggacc tgcgcgctgg atccgcgaaa ctccgcgatc
     4981 gggcgctgcg aatcgtcgcc gccgcgtgcg gcgtctctcc cgaacaggct cggcggctcc
     5041 tcgggcgttc ccgcggcgag gtccggacgg cgatcgtgat gggccggacg ggcgtaggtt
     5101 acgccgatgc ccggagtcgt ctcgacgccg caggcggaga cttgcggcgc gtcctcgaag
     5161 gctcgaagcc gcggtgacga agcgaagggc tccgccgaga ggagcgatgc tctccttcgt
     5221 ccacgagcat tcgctcagcc tggcggccat cgcgatcctc gcgacgtgga cgattctcta
     5281 cgcgcgatcc gaccccgata cccgctgggg cgagttcttc gggaacgccg tggcggactg
     5341 gagcggcacc ctgtttctcg tcctcgggac gaaactcctc tacgagcgtg gttctgtgga
     5401 aagccgccgc gcgccccggc actttcaaaa tcggacgatc gacctcctgt accaccactc
     5461 gctgacgatc ttcctcgtcg tcacgggagc catctggctg tttctgttcc accacgagaa
     5521 accgggctcg aagtggggac aggtctacgg aaacatcctc tcggagtggc tccagatgct
     5581 cgggatcgtc ttcctcacga aacgcctcct ggagcggggg tccaaggaga gccactagcc
     5641 cgatcggagt cgcgtccccg cgggcaggtc gagcagcacc gacaggcccg ggtgaagcgc
     5701 gagaaaggac gcgggacagc gcggcgtcac cggtccttcg agcgccgcgg cgacggccgc
     5761 cgccttcttc cgacccgtcg cgaccagcag gatcgtccgc gatgaaagga tcgtctcgat
     5821 cccgacggtg aacgcgtcgc gcggcaccgg gcgcactcca tcggccgcga ggcctcgccg
     5881 cgtcgaagcg gagagcgaga cgagcgacgt tcgcggcggc agagcccgcg ccggctcgag
     5941 ataggcgacg tgcccgttga tcccgacccc gacgaacgcg atgtccggtg ggccctcgcg
     6001 cgcgagcacc cgctcgtaac gggcgcactc gcgctccgcg tcgggcgcgt cgcccctaag
     6061 gaaccggatc cgccgccggt cgagatcgac tttcgagaag agatgccgct ccatgaacga
     6121 tcggaagctc cgcgggtcgt cggggggcac ggagagctcg tcgagattga aggtcgtcgc
     6181 ccggcggaag gatgcccggc cggccgcgtt caatcgggcg agctcgcgat agaccgggac
     6241 cgccgtcttg ccgacgggga gcagcaggac ggtcgcggcg cggcgccgga gaattccccc
     6301 gacacgctcc gccgccgccc gcgcgagagc ctccggcgtc gcgaagacct cgaccttcac
     6361 agctcgcgaa acggcacgag accgacgccc tgctcgacga gaaccgcctt cacatccgga
     6421 tcgcagagga gcgcgagctc gcgttcccgc tccccggcgt aggagctccc ggagagaagc
     6481 ccctcgtcga catgagcggg gtggcacatg agctccgtcg tctcgtgtgc cagtccgccg
     6541 atgatcgagc gaaggttctc cgcggtcgcc cccgcaccat ggaacccgtc gacgaagcgg
     6601 tccggcgtgc ggactccccg gccgcggagc gcgtctcgcg ccgcgacact cgaagcgcgc
     6661 acagggaccc cgagacgcag cgccgcgtcc gcgacgacgg gttgaacgga cgggtgaagc
     6721 gccgagtggt ggtgcgagtc gatgtgcgtc ggcggatcgc cgaccatctt ctcgaagagg
     6781 gagatctgcg cttctagctc gcgggcgact tcctcgggag cgagctcttc cggaaaatcc
     6841 gcaggaaagc gacggaagcg tccatcgacg aggagcgaac ggatgctctc gggagccgac
     6901 gccggaagcc cgcctccggt cagagcgaag tgaagtccga gcgacagccg cggcgcgcgg
     6961 cgccgggcgt ccgccacgcc ctccccggcc gccggctcga gcaccatcac cgttgcggag
     7021 gtcacgatcc cgaggaggtg cgcctcgacg acgccggcat tgacacccgg cgtccgcccg
     7081 aagtcgtcgg cgttgacgat gagccgcttc atccgagcgc ctcgatcgcg agcttcaccg
     7141 ctcccatcgc gggctcttcg acgagccggg agacgtgcgc gttggtgctc gcgagtcggc
     7201 gcgcgacgtc gtccgcgaga gtcggcaccg ccgcgaacgt gcctccggag agcacgacgt
     7261 cgtacgccga ttcttcgaga cgcagccggt cgcgcacgct cgccgcggcc cgcgtgagct
     7321 cccccgcggc gtccgaaagg atggccttcg cgacggcgtc tcccgctcgc gccgcttcct
     7381 cgacgcgcgg cgcgaacttc gcgacctgcg tcctcgggaa ttcgccgtcg tagatcgcgt
     7441 agaggatctg gtccggctgc gcgatcgaga aatgctcgga gagcgcccgg tcgagcgagg
     7501 tggcgcggcc gcggccgtcc gctccgcgga gcacgtcccg gat
//
LOCUS       DASWAV010000636         1705 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U2665
            k141_237989, whole genome shotgun sequence.
ACCESSION   DASWAV010000636 DASWAV010000000
VERSION     DASWAV010000636.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960316
            Sequence Read Archive: SRR10260007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 1705)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1705)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:47:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,929
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1705
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_237989"
                     /isolate="SMAG_U2665"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.36444 N 132.31889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..328
                     /gene="purH"
                     /locus_tag="VF958_13180"
     CDS             <1..328
                     /gene="purH"
                     /locus_tag="VF958_13180"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012470802.1"
                     /note="involved in de novo purine biosynthesis; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HEX9150109.1"
                     /translation="VAWKVVRRVKSNAIVLANARQTIGIGAGQMSRVDACRLAATKPV
                     LPIVNCGAASDAFFPFRDGLDVLAEAGVTAVVAPGGSIRDQEVVAAADQHQMAFLVAP
                     RRHFRH"
     gene            363..1076
                     /locus_tag="VF958_13185"
     CDS             363..1076
                     /locus_tag="VF958_13185"
                     /inference="COORDINATES: protein motif:HMM:NF015510.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA];
                     GO_process: GO:0044874 - lipoprotein localization to outer
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein carrier protein LolA"
                     /protein_id="HEX9150110.1"
                     /translation="MKRILFALALGSASAAAAQVRGAPSPAAKPVTPPAAITPSATSV
                     PDRASAALDSAVTRYSDGAAHSSPFVQIYTPSGFATARREAGTVSIQAPQRVRFDYTA
                     PEKKVFTYDTGEGRFFSPEDKQLTVRTLSEDEKARLPVVFLTRPADLGRQYEISVDSG
                     ETPSATTRLLLTPRVARPELAWLRLAIQNDGSVRELSYQDSAGNRTEFRFEDWKSGNA
                     RPAADFRITGPPGTRIIEN"
     gene            1180..1677
                     /locus_tag="VF958_13190"
     CDS             1180..1677
                     /locus_tag="VF958_13190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013120303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YajQ family cyclic di-GMP-binding protein"
                     /protein_id="HEX9150111.1"
                     /translation="MPQDFSFDVVNKVDLQEVTNAVQQASKEIATRFDFRGSASKVEL
                     REKELELVLVSDDEHKLKSVVDILETKLVRRGVAVKALDYGKVEPAAGATVRQVAKIQ
                     QGIPSEKAKAIVKAIKDRKLKVQASIQADQVRVVGRSKDDLQSAMSVVREGDFGLPLQ
                     FTNYR"
BASE COUNT          307 a          575 c          553 g          270 t
ORIGIN      
        1 ggtcgcctgg aaagtggtgc gccgggtcaa atcgaacgcc atcgtgctcg ccaacgcccg
       61 gcagacgatc ggcatcggcg ccggacagat gagccgagtc gacgcgtgcc gactcgccgc
      121 gacgaagccg gtgctcccga tcgtcaactg cggcgcggca tccgacgcct ttttcccgtt
      181 ccgggacggg ctcgacgtcc tcgccgaggc cggggttacg gcggtcgtcg ctcccggcgg
      241 ctcgattcgc gaccaggagg tggtcgccgc agccgatcag catcaaatgg ccttcctggt
      301 tgctccccga aggcatttcc ggcactgaat atccgcgcac gcgcgattgt tgtggctgct
      361 tcgtgaaacg aatcctgttc gcgctggcgt tgggaagcgc gtccgctgcg gctgctcagg
      421 tgcgcggcgc gccttcgccg gccgcgaagc cggtgacgcc gcccgccgcg atcacgccct
      481 ccgcgacgtc cgttccggac cgcgcctcag cggcgctcga cagcgccgtg acgcgctact
      541 ccgatggtgc cgctcactct tctccgttcg tccagatcta cacgccctcg ggattcgcga
      601 ccgcgcgacg cgaggccggg acagtctcga tccaggcgcc gcagcgggtc cgcttcgatt
      661 acacagcccc cgagaagaag gtcttcacct acgacacggg agagggccgg ttcttctcac
      721 ccgaagataa gcagctcacg gtgcgtacac tctcggaaga cgagaaagcg cgcctccctg
      781 tcgtcttcct cacgcggccg gcggacctcg gccggcagta cgaaatctcc gtcgactccg
      841 gcgagacacc gtcggcgacg acgcgtctcc tgttgacccc gcgcgtcgcg cggccagagc
      901 tcgcgtggct gcggctcgcg attcagaacg acggaagcgt tcgtgagctc tcctaccagg
      961 actccgccgg aaaccggacc gagttccggt tcgaggattg gaagagcggg aacgcgcgtc
     1021 ccgccgcgga cttccgcatc accggacccc cgggaacgcg aatcatcgag aactgacgtc
     1081 cgaagggcgt cattccgggg cgagaagcgc cgaaggaatc tgctgatata aggaagacgt
     1141 ccctcgccgc ctcacggctg gggacgacag ggtggtgaga tgccccagga tttctcattc
     1201 gacgtcgtca ataaggtcga cctgcaagag gtcacgaacg ccgtgcagca ggcgtcgaag
     1261 gagatcgcca cccgcttcga cttccgtggc agcgcctcga aggtcgagct ccgggagaaa
     1321 gaactggagc tcgtgctcgt ctccgacgac gagcacaagc tgaagtcggt cgtcgacatc
     1381 ctcgagacga agctcgtgcg ccgtggcgtc gcggtgaaag ctctcgacta cggaaaggtg
     1441 gaaccggcgg ccggcgcgac cgtccgccag gtcgcgaaaa tccagcaggg aatcccctcg
     1501 gagaaggcga aggcgatcgt caaggcgatc aaggaccgca agctgaaagt gcaggcttcg
     1561 atccaggcgg accaggtgcg cgtcgtgggg cgcagcaagg acgatctcca gagcgccatg
     1621 tcggtcgtcc gcgaaggcga tttcggcctg cccctgcagt tcacgaacta ccgatgatct
     1681 tctcgccgcg gtcccacaac ccccg
//
LOCUS       DASWAV010000637        13798 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U2665
            k141_393623, whole genome shotgun sequence.
ACCESSION   DASWAV010000637 DASWAV010000000
VERSION     DASWAV010000637.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960316
            Sequence Read Archive: SRR10260007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 13798)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13798)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:47:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,929
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13798
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_393623"
                     /isolate="SMAG_U2665"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.36444 N 132.31889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..531
                     /locus_tag="VF958_13195"
     CDS             <1..531
                     /locus_tag="VF958_13195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9150112.1"
                     /translation="VDVEIAHSDRLQSGRIVNVEPARERFRAGERVPVRVDVVDFRGG
                     LRRVMLELDVPSDTPPGPLTVFVGDGNSATAYDLAVYPADPQSLDQVLDFLSRMRPPN
                     SLNLIAYRRAAGAVVSGQPLAALPPSIAALIRDRGPGDGTPNLSYLRLQSSSVEQPVP
                     LTGSVQLNLEVLPNNW"
     gene            528..2708
                     /locus_tag="VF958_13200"
     CDS             528..2708
                     /locus_tag="VF958_13200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9150113.1"
                     /translation="MRVRASLRAAALVSAALVLSAGAASAATAKIWTSDSTADFSAGE
                     ARGVAVTVNGELVLSRGLERIEGVTEAVLYDAVSAPGGVVFVGTGEAGRILRISAAGK
                     VETYATLAEKQVTSLALGPDGALYAGASPGGKVYRIDSGKPSLYYDTKAQYVWSLAFK
                     GPALYVATGLPGEIHRVDASGRGTRLHQSSDPHVRTLHASGGDVWAGTSGSGLVLKVD
                     AGGKVTTVFDSGKSEVTAIASGPDGRVWAAVSSSEAAGGGEPISAPVPQPPKPAPRPS
                     GPGDEAPREKDKPEVTVTVSAPRLAPARPPTRTGFTSEVILFEKSEPPRVVWTGSDEM
                     VFDLGDADATSVLVGTGPKGRLYRVSADTWSLDRTLDEKQVTIVAGDAIATNGASAVY
                     RVRDGKRSGEYVSAVKDTGRTSTFGAFRYEGDVTAGAKLEVAFRSGESSAPDSTWSNW
                     SEFQDAAAAAKVAAPPGRYLQWKLRMTSGGAVPPVVRRVEAAYRNRNAGPVIESFLAM
                     GPNEVFARSGGSGSNVFETTAPDEKGIFTGLEEARAEPAPRKLLRKGYRTLTWKTTDA
                     DGDPVVHSLEFRPVSSSRWIPLKSDIRETFYSFDTTSLPDGEYVFRLSASDSEANPED
                     PKSASRETSPVAIDNTPPVIRRIDSARGVVRLEVVDAASPVTEFEYSVNAREWVRVEP
                     EDGLSDSRKETYRIELKADQRGGYLLVRATDAARNVAALSLTAP"
     gene            2759..4447
                     /locus_tag="VF958_13205"
     CDS             2759..4447
                     /locus_tag="VF958_13205"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX9150114.1"
                     /translation="MKRRLVPVLLLLLALAACRAKPAERPAAAVAPTPAAAPAPALGY
                     LDESHEGTPVEGGVLRRRLVGEPATLNAVLQSGLPEQQVLQYVSRQLLDFDARLELTG
                     NLTERWEVSADGREYRFFLRNDAVWEDGKPVSSGDAVFTIRRIVDRKVPAPVFKPLFG
                     TLETVEAIDGKSFRVRFRDRDALRLYAFALPLLPEHRFRGRPFLTASDNRAPLANGPY
                     RVEKWKTQESIDLVRNPRWNGAPAHFERIVLQIVPENSVAYRALVDGDLDETWIDQPL
                     KERSENDSRFHDCCRVVEFYNLDYNYIGLNNRSPFFSDARVRRAMTMLLDRPAIVRSL
                     YHGSARIISGPWAPDSPAYDSTVTPLPFDPRAAGQLLDQAGWRDTNGNGIRDRDGREF
                     EFDLLLSAGSNVGRQIDEIFTAELARAGVQARIRSLEWATFTERVDAGEYEAATLAWA
                     AADPNPDPLPYWHSSQFPPQGLNSAWYSNPAADRLMEEARGELDREKRRAIYHRLHRV
                     LRDNPPVVFVVNASQKYAFRRRVHGLVTSPLGLYGFSPGPLSWWGSPDPKPRAR"
     gene            4444..5427
                     /locus_tag="VF958_13210"
     CDS             4444..5427
                     /locus_tag="VF958_13210"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX9150115.1"
                     /translation="MIRYLLRRIVLAIPTLAGIVIVVFLLLHLAPGSPTSAAGSAESG
                     RRVSAEAREEMRRLYGLDRPLPEQFAKWIGRVARFDLGESFVDRRPVSERIREALPAT
                     LALNGLALFFTLLIAVPIGVAAGGRPDGRFDRTSAVVLFALYSMPVFWAALLLQTLLS
                     VELGWLPLYGIASDPAPAGLAGMADRLTHLALPVTCLTYGSLAFLARLVRAGVVEARS
                     QDYVTAALARGVSRRAALWRHAFRNALLPLITLAGLLLPGLLSGSVIVERIFAWPGLG
                     RLYFDSILSRDYPVVLGLSLVGAVATLAATLAADVAYAFADPRVREAGEAA"
     gene            5424..6230
                     /locus_tag="VF958_13215"
     CDS             5424..6230
                     /locus_tag="VF958_13215"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX9150116.1"
                     /translation="MIRRLPGSGRVALAVVGLLWIVAALSPLFRARAGDIRLEERLRP
                     PSLTHPFGTDDLGRDLAARVIVATPISLAIGLLAATMSLAVGFGIGGAAGYFGGAADL
                     LLSRLIEVVLCFPVLFLLLALAAFLPASAATVVLAIGLTTWPADARYARAEILKARSL
                     DYTRAARAAGASPARILLRHLLPNALPPLLVSAAFGVSWAILAEAALSFLGVGLPAPA
                     ASWGGILSAAPAYIEEAWWLALFPGIALFATVSAYNLLAEGLRQATTKAD"
     gene            6236..6715
                     /gene="coaD"
                     /locus_tag="VF958_13220"
     CDS             6236..6715
                     /gene="coaD"
                     /locus_tag="VF958_13220"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020707302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004595 - pantetheine-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HEX9150117.1"
                     /translation="MPERVALYPGSFDPLTNGHLDVLFRARRLADRVIVAILENDEKK
                     PLFSLEERLEMIREAVGDDPSVSVHTFSGLLVDFAAESGATLLVRGLRAISDYEYELQ
                     MALMNRRLAPSIETVFLMAKEEYSYVSSRLVKEVARLGADVSGLVPEAVRRRLAEMF"
     gene            6712..7122
                     /locus_tag="VF958_13225"
     CDS             6712..7122
                     /locus_tag="VF958_13225"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009509426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HEX9150118.1"
                     /translation="MKTVETRLRVRYSETDRMGIVYHGHYIAWFEIGRTEYCRAAGVP
                     YRALEDSGLLILVTSVECKYRRSARYDDEVRVATSLSELASRGLAFTYEVFDAGARLL
                     ARGATRHVFADAAGKPVRAPAAVIETLSAFRASD"
     gene            complement(7085..7798)
                     /locus_tag="VF958_13230"
     CDS             complement(7085..7798)
                     /locus_tag="VF958_13230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019947339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEX9150119.1"
                     /translation="MKVSVVIPVFNERESLPLVVADLPRDRVTEVVVVDNGSTDDTSA
                     VARGLPVRLVREERRGYGSACLAGIAALKSSPPDVVVFLDGDYSDHPEELPLLLDGIA
                     AGSDLVIGSRMLGSREPGALLPQARFGNALACSLVRLIFGYKYTDLGPFRAIRWEALE
                     RLGMRDRDFGWTVEMQVKALTAGLRVAEVPVSYRRRVGVSKITGTLAGTLKAGSKILR
                     TIARYGVLSRSRGTRRASQ"
     gene            complement(7795..8718)
                     /locus_tag="VF958_13235"
     CDS             complement(7795..8718)
                     /locus_tag="VF958_13235"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEX9150120.1"
                     /translation="MPAEQSNSASTPAWKIHAALAGAQAGFALFPIFGKLALVSIPPF
                     SLAAIRVVTSAIMLEVVRRLSPEERLAVSDRGRIFLLALLGVSFNQVLFILGLSLTTA
                     INTSILISAIPVFTLATAVLLGREQATARAIGGIVLAAAGALVLLNAERFEWSSRYFR
                     GDLAILGNGFLYSVYLVLSRPMLARYRAITFTSAVFRWGAAPIVLASIPALLRFSPAS
                     VSPSAWWSLGAVIVFCTVIPYLLNSWALARTEASRVATYVFLQPVIASTLAMLILGER
                     ATWRTAVAAALIFSGLAVTLRRARLPARAAS"
     gene            complement(8728..9627)
                     /locus_tag="VF958_13240"
     CDS             complement(8728..9627)
                     /locus_tag="VF958_13240"
                     /inference="COORDINATES: protein motif:HMM:NF013720.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9150121.1"
                     /translation="MDLPGYAAARERIAHRFRPAGILDVTGADRESFLQGQLTQDVRG
                     LVPGASRRAAGLTPKSKLLFVARVVALEDRLRLVVPEAQRQVVLDHLRKYAVFTKVAF
                     EDRSEQLLQIGLYGPQGAGLALPDGIDRLPPEGEFSASLIVPVDRRGEIEAHLAAAGS
                     VEVSEEAAEVLRVEAGRPRFGFDMDSSNLPDEVGMDDAISTTKGCYVGQEVVARLRTY
                     GRVNRRLVRFHFPEGPIGAGEVLRRPDEESPSSTEAGRVTSSVVSPLSGAIGLGYAFR
                     DIPVGAPLFSVSKPQLPAVVGER"
     gene            complement(9659..10117)
                     /locus_tag="VF958_13245"
     CDS             complement(9659..10117)
                     /locus_tag="VF958_13245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9150122.1"
                     /translation="MNPRRTVLALLALFAGSAGLFAADGSFEKTIAVPRRADARLGWS
                     YRGCSIESLTLRNYPDDDDIAEARAKDTGDKSWLWWEFNVANKSDRKCRIRLSLEIFD
                     KKGNVVKSSDRSGSVSPGELDDDIRVSTLMKTLDIADAAKVRVRATIGPD"
     gene            10167..10448
                     /locus_tag="VF958_13250"
     CDS             10167..10448
                     /locus_tag="VF958_13250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9150123.1"
                     /translation="MSAPQGGADLHVIDIFMMSIRCGNCDTYQTVVGFQKLPDKNVYT
                     YECENEICDPNVTRTIVEVPKDFDNSTKRAEELPYYTADEAPEEDPGLK"
     gene            10461..10934
                     /locus_tag="VF958_13255"
     CDS             10461..10934
                     /locus_tag="VF958_13255"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF025892.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HEX9150124.1"
                     /translation="MSSAAGPHPVPVLAPDLADRLRVRLAASPALQWLQPELLELDLD
                     YCVLGLKYRSEVTNGSSTMHGGVLATLADTAVAFALSTNFDGKMGFATTDLTIHFLRR
                     AKTDVRARARILKKGRRVTVGEVDIVDADGHDVARVLTSFLLTTSSFDYLPGARQ"
     gene            10931..11920
                     /locus_tag="VF958_13260"
     CDS             10931..11920
                     /locus_tag="VF958_13260"
                     /inference="COORDINATES: protein motif:HMM:NF014045.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HEX9150125.1"
                     /translation="MTDEPVPFPAAEAAEGEKKPRRGRRGGVRHRKARVKEEPPPAHA
                     KPAHSRPATAPPALLAAAVAPPPASAAVSFGGTPTLPARPAVEQRLAVFIDFENIALG
                     ARDAQYKKFDINLVLQRLIEKGRIVFKKAYADWTRYAEYKREFHEAAIELVDIPQRGY
                     TGKNSADIRMVVDAMDLVGAKAHITTFVILSGDSDFSPLVSKLKESDKSVIGVGVKNS
                     TSDLLISNCDEFIFYEDLVRETRALPSIENLPKKQQDVFRLVLDSIQALQRENYDVIW
                     GSMIKQTMQRKQPYFNETYYGYKSFSELLEDAQRKGLFTLKKDAKSGGYIVSV"
     gene            complement(12094..13149)
                     /locus_tag="VF958_13265"
     CDS             complement(12094..13149)
                     /locus_tag="VF958_13265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014810513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9150126.1"
                     /translation="MSKIVHVVGTGTIGEPLIGFFADFKRHLGIDEITFHKRTPMTTE
                     RAKVNHLMRRGAHLAVDPDKTEDFESLGHKVSFDAREALERATVVIDCTPAGNENKKL
                     YQTIDGPNGYIAQGSEFGFGKPFAIGINEEALVPGEDRFVQIVSCNTHNIATLIKTLG
                     SNDGEPVLESGRFVCMRRANDVSQDSSFCPAPTAGKHDDPVFGTHHARDAYHLFETIG
                     WKPRIFSSAMKLNTQYMHSLWFDMELSEPFTREKALQKLQCNTRIAMTEKRSANQIFS
                     FGRDHGYYGRIFSQTVVAKPTVTVNGDREVIGFCFTPQDGNSLASSIAAALWIVDPEF
                     DKAERLSVLDRFFFREI"
     gene            13256..>13798
                     /locus_tag="VF958_13270"
     CDS             13256..>13798
                     /locus_tag="VF958_13270"
                     /inference="COORDINATES: protein motif:HMM:NF023568.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEX9150127.1"
                     /translation="MSARLHAFLFPGPTGQLEGLWKGAENGHAGSAVFAHPHPVYGGT
                     LHSKVVFRAARTLSRAGYGVLRFNFRGVGLSEGRYDEGRGETADYRAALDEAERRGGQ
                     PIVAGGFSFGSAVGLKAIEGDPRVVAFIGVGVPLASDVGADLPFPKVPALFVVGKDDV
                     FGPPARLREWAAGRGLVVEVA"
BASE COUNT         2120 a         4761 c         4701 g         2216 t
ORIGIN      
        1 gtcgacgtcg agatcgcgca ctccgacagg ttgcagagcg gccgcatcgt caacgtcgag
       61 ccggcccggg agcgcttccg agcgggcgag cgagtgccgg tgcgcgtcga cgtcgtggac
      121 ttccgcggtg gactccggcg agtcatgctc gagctcgacg ttccgagcga cacgccgccc
      181 ggcccgctga ccgtgttcgt cggtgacgga aactccgcga ccgcgtacga cctcgccgtc
      241 tacccggccg acccgcagtc gctcgatcag gtgctcgatt tcctgtcgcg catgcggccg
      301 ccgaacagcc tgaacctgat cgcttaccgc cgcgcggcgg gcgcggtcgt gtcggggcag
      361 ccgctcgccg cgctgccgcc cagcatcgcc gcgctcatcc gcgaccgcgg cccgggagac
      421 ggcacgccga acctttcgta tctgcgcctc cagtcgagct ccgtcgagca gcccgtgccc
      481 ctgaccgggt cggtccagct caacctggaa gtcctgccga acaactggtg agagtgaggg
      541 cgtctctccg cgccgcggcg ctcgtttccg cggcgctcgt cctgtccgcc ggtgcggcgt
      601 ccgccgccac agcgaagatt tggacctcgg actccaccgc cgacttctcg gcgggcgagg
      661 cccgcggcgt cgcggtgacc gtcaacggcg agctcgttct ctcgcgcggg ctggagcgca
      721 tcgaaggggt gaccgaggcc gtcctctacg acgcggtgag cgcgccgggc ggcgtcgtct
      781 tcgtcggaac gggcgaggcg ggacggatcc tgcggatctc cgccgccggc aaggtcgaga
      841 cttacgccac gctggccgag aaacaagtca cgtcgctcgc actcggaccc gacggcgcgc
      901 tctacgccgg tgcgtcgccg ggaggaaagg tctaccggat cgactccggg aagccttcgc
      961 tctactacga cacgaaagcc cagtacgtgt ggtcgctcgc cttcaaaggg cccgcgctgt
     1021 acgtcgcgac cgggctgccc ggggaaattc accgggtcga cgcctccgga cgcggcacgc
     1081 ggctccacca gtcgtcggac ccgcacgtcc ggacccttca cgcgtcggga ggcgacgtgt
     1141 gggcggggac gtcggggtcc ggcctcgtcc tcaaggtgga cgcgggcggc aaagtgacga
     1201 cggtcttcga ctccggaaag tcggaagtga cggcgatcgc gtccggcccg gacggccgcg
     1261 tctgggcggc tgtttcctct tccgaggcgg ccgggggcgg cgagccgatc tcggctcctg
     1321 tcccgcagcc gcccaagccg gccccccgtc cgagcgggcc cggagatgag gcgccgcgcg
     1381 agaaagacaa gccggaggtg acggtcaccg tctccgctcc gcggctcgca ccggcgaggc
     1441 ctcccacccg gaccggcttc acctcggagg tcatcctgtt cgagaaaagc gagccgccgc
     1501 gggtcgtgtg gacgggctcg gacgagatgg tcttcgacct cggggacgcc gacgcgactt
     1561 ccgtgctggt cgggacgggc ccgaagggtc gcctttatcg ggtatccgcc gacacgtggt
     1621 cgctcgaccg gacgctcgac gagaagcagg tcacgatcgt cgccggcgat gcgatcgcca
     1681 cgaacggagc gagcgcggtt taccgcgtgc gagacggaaa gcgaagcggg gaatacgtct
     1741 cggccgtcaa ggacaccggg cggacgagca ccttcggcgc gttccgatac gagggggacg
     1801 tgaccgcggg cgcgaagctc gaagtcgcgt tccgctcggg tgagtcgtcg gcgcccgact
     1861 cgacgtggag caactggtcc gaattccagg acgcggccgc ggccgccaag gtcgccgccc
     1921 cgcccggccg ctatctccag tggaagctgc gcatgacgtc ggggggcgca gtgccccccg
     1981 tggtccgccg cgtcgaagcg gcgtaccgca accgcaacgc cgggccggtc atcgaatcgt
     2041 tcctggccat gggtccgaac gaggtgttcg cgcgttcggg ggggtccggc tccaacgtct
     2101 tcgaaactac ggcgcccgac gagaagggga tcttcaccgg cctcgaggaa gcccgggccg
     2161 aaccggcgcc gcgcaagctc ctgcggaagg ggtatcggac gctgacgtgg aagaccaccg
     2221 acgccgatgg cgacccggtc gtccactcgc tcgagttccg gccagtgtct tcttcgcgct
     2281 ggatccccct gaagtcggac atccgggaga ccttctactc cttcgacacg acgtcgctgc
     2341 ccgacggcga gtacgttttc cggctctccg cgtccgactc cgaggcgaat cccgaggatc
     2401 cgaagtccgc gagccgggag acgagcccgg tcgcgatcga caacactccg cccgtcatcc
     2461 gccggatcga ctcggctcgg ggggtcgtcc gcctcgaggt cgtggacgcg gcgtcgccgg
     2521 tcaccgaatt cgagtacagc gtcaacgcgc gcgagtgggt gcgcgtcgag cccgaggacg
     2581 ggctttccga ctcccgcaag gagacgtaca ggatcgagct gaaggccgac cagcggggcg
     2641 gatacctcct cgtccgcgcg accgacgccg cgcgaaacgt ggcggcgctg tcgctgacgg
     2701 ctccctgagg cgggaaacgg aaaacgggaa agggatcacc acccggcata attgccgcgt
     2761 gaagcggcgc ctcgtcccgg tcctcctgct cctgctcgca ctcgcggcat gccgcgcgaa
     2821 gcctgcggag cgcccggcgg ccgccgtcgc gcccaccccc gccgcggctc ccgcgcccgc
     2881 gctcggctac ctcgatgaat cgcacgaggg cacgcccgtc gaagggggag tcctgcgccg
     2941 gcgcctcgtc ggcgaacccg cgactttgaa cgcggtcctc cagtcgggcc tgccggagca
     3001 gcaggtcctt cagtacgtct cgcgccagct cctcgatttc gacgcccgac tcgagctcac
     3061 cgggaacctc acggagcgct gggaggtgtc cgccgacggc cgcgagtacc gctttttcct
     3121 tcggaacgac gccgtctggg aggacgggaa gcccgtgagc tccggcgatg cggtgttcac
     3181 gattcggcgc atcgtggacc gcaaggtccc ggcccccgtc ttcaagccac tcttcgggac
     3241 cctcgagacc gtcgaggcga tcgacgggaa gtccttccgg gtccggttcc gcgaccgcga
     3301 cgcgctgcgt ctctacgcct tcgcgctccc gcttctcccc gagcaccggt tccggggacg
     3361 gccgttcctc acggcgtccg acaaccgcgc accgctggcg aacggccctt accgcgtgga
     3421 gaagtggaag acgcaggagt cgatcgatct cgtgcggaat ccccgctgga acggcgcgcc
     3481 ggcgcatttc gaacggatcg tcctgcagat cgttccggag aactcggtgg cgtaccgcgc
     3541 gctcgtcgac ggggacctgg acgaaacgtg gatcgatcag ccgctgaagg agcggagcga
     3601 aaacgacagc cggttccatg actgttgccg ggtcgtcgag ttctacaacc tcgactacaa
     3661 ctacatcggt ctaaacaacc gttcgccttt cttttccgac gcgcgcgttc gccgcgcgat
     3721 gacgatgctc ctcgatcgcc ccgcgatcgt ccgcagcctc taccacggct ccgcccgcat
     3781 catctccggg ccgtgggcgc ccgattcgcc cgcgtacgac tcgaccgtca cgccgctccc
     3841 gttcgatccg cgggcggcgg gccagctgct cgaccaggcg ggatggcggg acaccaacgg
     3901 caacgggata cgggatcggg atggccggga gttcgagttc gacctgctcc tctcggccgg
     3961 ctcgaacgtc ggccgccaga tcgacgagat cttcacggcg gagctcgccc gagcgggcgt
     4021 gcaggcgcgt atccggtcgc tcgagtgggc gacgttcacc gagcgggtcg acgccggcga
     4081 gtacgaagcg gcgacgctcg cgtgggcggc cgcggacccg aaccccgatc cgcttcccta
     4141 ctggcactct tcgcagtttc cgccgcaggg gttgaacagc gcctggtact cgaacccggc
     4201 tgcggaccgg ctgatggagg aggcgcgcgg ggagttggat cgggagaagc ggcgcgccat
     4261 ttaccaccgg ctccaccggg tgctgcgcga caatcccccg gtcgtgttcg tcgtgaacgc
     4321 atcgcagaag tacgcgttcc gccgccgtgt ccacgggctc gtgacgtcgc ccctcggtct
     4381 ctacggcttc tcgccggggc cgctctcgtg gtgggggtcg cccgatccaa agccgcgagc
     4441 gcggtgatcc gctacctgct ccggcggatc gttcttgcga tcccgaccct cgccgggatc
     4501 gtcatcgtcg tgtttcttct ccttcacctc gcgccgggga gcccgacctc agccgccggg
     4561 tcggcggagt ccggccgccg ggtctccgcg gaggcgcggg aagagatgcg gaggctctat
     4621 ggcctcgacc ggccgcttcc ggaacagttc gcgaagtgga tcggccgcgt ggcgcggttc
     4681 gacctcgggg aatcgttcgt cgaccgccgg ccggtctcgg agcggatccg cgaagctctc
     4741 ccggcgacgc tcgcgctgaa cggcctcgcg ctcttcttca cactcctgat cgcggtgccg
     4801 atcggcgtcg cggcaggggg ccggccggac ggccggttcg accggacttc ggccgtcgtc
     4861 ctcttcgccc tgtactccat gccggtcttc tgggcggccc tccttctcca gacgctcctc
     4921 tcggtcgagc tcgggtggct gccgctctat ggcatcgctt ccgatccggc tccggccggt
     4981 ctggcgggca tggccgaccg actgacgcat ctcgcgctcc ccgtcacgtg cctgacctat
     5041 ggctcgctcg cgtttctcgc acggctcgtc cgggcgggcg tcgtcgaggc gcgttcccag
     5101 gactacgtga cggcggcact cgcccggggc gtctcccggc gcgccgcgtt gtggcggcac
     5161 gccttccgca acgcgctcct gccgctcatc acgctcgcgg gactcctcct gccgggcctg
     5221 ctgtcgggga gtgtcatcgt cgagcggatt ttcgcgtggc cgggcctcgg ccggctctac
     5281 ttcgactcga tcctctcgcg cgactatccg gtcgtgctcg ggctctcgct cgtcggcgcc
     5341 gtcgcgaccc tcgccgcgac cctcgcggcc gacgtcgcgt acgcgttcgc ggatccgcgc
     5401 gtgcgcgagg cgggggaggc tgcgtgatcc gacgactgcc ggggagcggt cgggtggccc
     5461 tcgcggtcgt cggcctgctc tggatcgtcg ccgcgttgtc gccgctcttc cgggcgcgag
     5521 ccggcgacat tcgcctcgag gagcgtctcc gacctccctc gctcacgcac ccgttcggga
     5581 ccgacgacct cggccgcgat ctcgcggccc gcgtgatcgt cgcgacgccg atctccctcg
     5641 cgatcggcct cctcgccgcg acgatgtcgc tcgcggtcgg cttcgggatc ggcggcgcgg
     5701 ccggttactt cgggggcgcg gccgaccttc tcctctcgcg gttgatcgag gtcgtcctct
     5761 gctttccggt gctgttcctg ctcctcgcgc tcgccgcgtt cctgcccgcg tccgccgcga
     5821 cggtcgttct cgccatcggg ctgacgacgt ggcccgccga tgcccgctac gcccgcgcgg
     5881 aaatcctgaa ggcgaggagc ctcgactaca ctcgggcagc ccgggcggcc ggagcgagcc
     5941 cggcgcgcat tctcctgcgc cacctgctgc cgaacgcgct gcccccgctt ctcgtttccg
     6001 ccgcgttcgg ggtctcctgg gcgatcctgg cggaggccgc gctttcgttc ctcggagtcg
     6061 ggctgccggc cccggccgct tcgtggggcg ggatcctgtc ggccgcgccc gcctacatcg
     6121 aagaggcctg gtggctcgcg ctctttccgg gcatcgccct cttcgcgacg gtgtctgcct
     6181 ataatctgct ggccgaaggc ctgcgacagg cgacgacgaa agcggactga accaaatgcc
     6241 cgaacgcgtg gccctctatc ccggctcctt cgatccgctc accaacggac acctcgacgt
     6301 gctcttccgc gcccggcggc tggccgaccg ggtgatcgtc gcgattctcg agaacgacga
     6361 gaagaagccg ctcttctccc tcgaggaacg cctcgagatg atccgcgagg ccgtcgggga
     6421 cgacccgtcg gtctcggtgc acacgttctc gggcctgctc gtggattttg cggccgagtc
     6481 cggcgcgacg cttctcgttc gcgggcttcg cgcgatttcc gactacgagt acgagctcca
     6541 gatggcgctg atgaatcggc gcctcgcgcc ctcgatcgag accgtgttcc tcatggcgaa
     6601 ggaggagtat tcgtacgtct cgagccggct cgtgaaggaa gtcgcgcggc tcggggcgga
     6661 cgtttccggg ctcgtgccgg aggcggtccg ccgccggctc gccgaaatgt tttgaagacc
     6721 gtcgagacac gcctccgggt ccggtactcc gagaccgacc ggatggggat cgtctatcac
     6781 ggccactaca tcgcctggtt cgaaattggc cggacggagt actgcagagc cgccggcgtg
     6841 ccctatcgag ccctggagga ctcgggcctc ctgatcctcg tgacgtcggt ggagtgcaag
     6901 tatcgccggt cggcgcgcta cgacgacgaa gtccgcgtcg cgacttcgct ctcggagctc
     6961 gcgagccgtg ggctcgcgtt cacctacgag gtcttcgacg cgggagctcg tctcctcgcc
     7021 cggggcgcga cccggcacgt gttcgccgac gcggccggga aaccggtccg ggcgccggcc
     7081 gccgtcattg agacgctctc cgcgttccgc gcgagcgact gagaacgccg tagcgcgcga
     7141 tcgtccgcag gatcttcgat cccgccttga gcgtgccggc cagcgtgccg gtgatcttgg
     7201 acaccccgac ccgccgccgg taggagacgg ggacctccgc gacccgcagg ccggccgtga
     7261 gtgctttcac ctgcatctcg acggtccagc cgaagtcgcg gtccctcatc ccgagccgct
     7321 cgagcgcctc ccaccggatc gcgcggaatg ggccgaggtc ggtgtacttg tagccgaaga
     7381 tcagccggac gagcgagcac gccagggcgt tgccgaaacg cgcctggggg agcagcgccc
     7441 ccggttcgcg agatccgagc atccgcgagc cgatgacgag gtccgacccc gccgcgattc
     7501 cgtcgaggag gagcggcagc tcctcggggt ggtcggagta gtcgccgtcg aggaacacga
     7561 cgacgtcggg gggcgacgac ttcagtgccg caatcccggc gaggcaggcg ctcccgtacc
     7621 cgcgccgctc ctcgcggacg agcctcacgg gaagaccgcg cgcgacggcg gacgtgtcgt
     7681 cggtcgagcc gttgtccacg acgacgacct cggtgacgcg gtcgcgcgga aggtccgcca
     7741 cgacgagcgg cagggactcc cgctcgttga aaaccggaat gacgaccgag accttcacga
     7801 cgccgcgcgc gccgggagcc gggcgcgccg cagggtcact gcgagccccg agaaaatgag
     7861 cgccgccgcc acggccgtgc gccacgtggc gcgctctccg aggatcagca tcgcgagcgt
     7921 ggacgcgatc accggctgca ggaagacgta cgtcgctacg cgcgacgcct ccgtccgtgc
     7981 gagcgcccag gagttcaaga ggtagggaat cacggtgcag aaaacgatca ccgcgccgag
     8041 actccaccag gcgctcggcg acaccgaggc cggagagaag cgcaggaggg ccgggatcga
     8101 agcgagaacg atgggcgccg ctccccagcg aaagacggcg gacgtgaagg tgatcgcgcg
     8161 gtaccgcgcg agcatggggc gagagaggac gaggtagacc gagtacagga agccgttccc
     8221 gaggatcgca aggtccccgc ggaagtagcg gctgctccac tcgaagcgct ccgcgttcag
     8281 cagcacgagg gcgccggccg cggcgaggac gatgccgccg atcgcacggg ccgtcgcctg
     8341 ctcccggccg agcaggaccg ccgtcgccag ggtgaacacg gggatggccg agatcaggat
     8401 cgaggtgttg atcgcggtcg tgagtgagag gccgagaatg aagaggacct ggttgaacga
     8461 gacgcccagc agcgcgagga gaaagatccg gccgcggtcg gagacagcga ggcgttcctc
     8521 cggagacagc cggcgcacga cctcgagcat gatcgccgag gtcacgacgc gaatcgccgc
     8581 gagcgagaag ggcggaatgg agacgagcgc gagctttccg aagatcggaa agagcgcgaa
     8641 acccgcctgc gctccggcga gggcggcgtg aatcttccac gccggggtcg acgcggaatt
     8701 gctctgttca gccggcaatg cgagggttca gcgctcgccc acgacggcgg gcagttgcgg
     8761 tttcgagacg gagaagagcg gcgcgccgac gggaatgtcg cggaaggcgt acccgagacc
     8821 gatcgcgccg gacagcggcg acacgacgga gctcgtcacg cgacccgcct cggtcgacga
     8881 cgggctctcc tcgtcgggcc gccgcaggac ctcgcccgcc ccgatcggcc cctccgggaa
     8941 gtggaatcgg acgaggcgcc ggttgacccg cccgtacgtc cggagccggg ccacgacctc
     9001 ctggccgacg tagcagccct tcgtcgtcga gatcgcatcg tccatcccga cttcgtcggg
     9061 caggttcgaa gagtccatgt cgaatccgaa cctcggtcgc cccgcctcca cgcggagaac
     9121 ctcggccgct tcctcggaga cctcgaccga gcccgcggcg gcgagatgcg cctcgatctc
     9181 cccccggcga tcaacgggga cgatgaggct cgccgaaaat tcaccctcgg ggggcagccg
     9241 atcgatgccg tcgggaaggg ccaggccggc gccctgcggt ccgtagagcc cgatctgtag
     9301 cagctgctcc gaccggtcct cgaacgcgac cttcgtgaat acggcgtact tgcgcaggtg
     9361 atcgaggacg acttgtcgct gcgcttccgg cacgacgagg cgcagccggt cctcgagggc
     9421 gacgacccgg gcgacgaaga ggagcttgct cttcggcgtg aggccggcgg cgcgacggct
     9481 cgcgcccggc acgaggccgc gcacgtcctg cgtgagctgc ccctggagaa acgactcccg
     9541 gtcggcgccc gtgacgtcga ggatcccggc cgggcggaac cggtgagcga tcctttctcg
     9601 cgcggcggcg tagccgggca ggtccatcgc gcgaattgta ggttgtgcgg tgaccgaact
     9661 agtcaggacc gatcgtcgcc cgcacgcgca ctttcgcggc gtcggcgatg tcgagggtct
     9721 tcatgagcgt cgaaacacgg atgtcgtcat cgagctctcc cggggagacg gagccgcttc
     9781 ggtcgctcga cttgacgacg ttgcccttct tgtcgaagat ctcgagcgag agccggatcc
     9841 ggcacttccg atcgctcttg ttcgcgacgt tgaactccca ccagagccac gacttgtcac
     9901 cggtgtcctt cgcgcgggcc tcggcgatgt cgtcgtcatc ggggtagttg cgcagcgtca
     9961 ggctttcgat cgaacatccc cggtagctcc agccgagccg cgcgtccgcg cgtcgcggga
    10021 ccgcaatcgt cttctcgaat gagccatccg cggcgaagag acctgcggaa cccgcgaaga
    10081 gcgccaggag agcgaggacc gtgcgccttg ggttcatgtc cgcctcgatt cgagaggata
    10141 gggttagctc atcgtatcat ccgagcgtga gcgctccgca aggcggcgcc gacctccacg
    10201 tgatcgacat cttcatgatg tcgatccggt gcggcaactg tgacacctac cagacggtcg
    10261 tcggcttcca gaagctgccg gacaagaacg tctataccta cgagtgcgag aacgaaatct
    10321 gcgacccgaa cgtgacgcgc acgatcgtcg aggtccccaa agatttcgac aactccacga
    10381 agcgcgccga ggagctgccg tactacacgg ccgacgaggc gccggaggaa gaccccgggc
    10441 tcaagtagaa tgagtcgttt atgagctccg ccgccggacc acaccccgta cccgtgctcg
    10501 cgccggacct tgcggatcgg ctgcgggtcc gactggcggc ctctcccgcg ctccagtggc
    10561 tccagcccga actcctcgag ctcgatctcg attactgcgt cctcggcttg aaataccgtt
    10621 ccgaggtcac gaacggctct tccacgatgc acggcggagt cctcgcgact ctcgccgaca
    10681 cggcagtggc gttcgcgctc tccacgaact tcgacggcaa aatgggattc gcgacgaccg
    10741 atctgaccat ccactttctg cgccgcgcga agacggacgt ccgagcgcgc gcgcggatcc
    10801 tgaaaaaggg gaggcgggtg acggtggggg aggtggacat cgtcgacgcg gacggccacg
    10861 acgtggcacg cgtgctgacg agcttcctcc tcacgacttc ctcgttcgac tatctccccg
    10921 gagcaagaca atgaccgacg aacccgttcc cttcccggcg gccgaggcgg ccgaggggga
    10981 gaagaaaccc cgtcgcggcc ggcgtggcgg cgtgcgccac cgcaaggccc gcgtcaagga
    11041 agagccgccg cccgcgcacg ccaagccggc gcactcccgt ccggcgaccg ctcctcccgc
    11101 gcttctcgcg gccgcggtcg ctccgccgcc ggcttcggcc gccgtctcgt tcggtgggac
    11161 gcccaccctg ccggcgcggc ctgcggtcga gcagcgcctc gcggtcttca tcgacttcga
    11221 gaacatcgcg ctcggcgcac gcgacgcgca gtacaagaag ttcgacatca acctggttct
    11281 gcagcggttg atcgagaagg gccggatcgt cttcaagaag gcgtacgccg actggacgcg
    11341 atacgccgag tacaagcggg agttccacga ggccgcgatc gagctcgtgg acatcccgca
    11401 gcgcggctac accggcaaga actccgccga catccgcatg gtcgtggacg cgatggacct
    11461 cgtcggcgca aaggcccaca tcacgacctt cgtgattctg tcgggcgact ccgacttctc
    11521 tccgctcgtc tcgaagctga aggagagcga caagagcgtg attggcgtcg gggtcaagaa
    11581 ctcgacctcg gacctgctga tctccaactg cgacgagttc atcttctacg aggacctcgt
    11641 gcgcgagacg cgggcgctcc cgtcgatcga gaaccttccg aagaagcagc aggacgtctt
    11701 ccggctggtt ctcgactcaa tccaggcgct gcagcgtgag aactacgacg tgatctgggg
    11761 ctcgatgatc aagcagacga tgcagcgcaa gcagccctat ttcaacgaga cgtactacgg
    11821 ttacaagtcg ttctcggagc tcctggaaga cgcccagcgc aagggcctct tcaccctgaa
    11881 gaaagacgcg aagagcgggg gctatatcgt ctccgtatag ccgtcgcggg cgcgtcgctt
    11941 cggacctgtt acgcaccggc gagcggctcg ctatccccgg ctcgaccgct cgcctggtcc
    12001 tcgcttccgt gcccgccccc gcggcgcaag gctcctcgct cgattacctc gcgagactcc
    12061 tcgcgccgcg aacggcgacc ggttagccgg gactcagatt tcgcggaaga agaaccggtc
    12121 gagcaccgag aggcgttccg ccttgtcgaa ctcggggtcg acgatccaga gcgccgccgc
    12181 gatcgacgag gcgagcgagt tgccgtcctg gggcgtgaag cagaacccga tcacctcgcg
    12241 gtcgccattc acggtcacgg tcggctttgc gacgaccgtc tgggagaaga ttcgcccgta
    12301 gtagccgtgg tcgcggccga aggaaaagat ctggttggcg gacctcttct cggtcatcgc
    12361 aatccgcgtg ttgcactgga gcttctggag cgccttctcg cgggtgaacg gctcggagag
    12421 ctccatgtcg aaccagagcg agtgcatgta ctgggtgttc agcttcatcg cggaggagaa
    12481 gatccgcggc ttccagccga tggtctcgaa cagatggtag gcgtcgcgcg cgtgatgggt
    12541 gccgaacacc gggtcgtcgt gcttgccggc agtcggcgcg ggacagaagc tcgagtcctg
    12601 cgagacgtcg ttcgcccttc gcatgcagac gaaccgtccg ctctcgagca cgggctcacc
    12661 gtcgttcgac ccgagcgtct tgatgagggt cgcgatgttg tgggtattgc aggagacgat
    12721 ctggacgaaa cggtcttccc cgggaacgag cgcctcctcg ttgatcccga tcgcgaaggg
    12781 ctttccgaac ccgaactcgc tgccctgcgc gatgtacccg ttggggccgt cgatcgtctg
    12841 gtagagcttc ttgttctcgt tgcccgcggg cgtgcagtcg atgacgactg tcgcgcgctc
    12901 gagcgcttcg cgcgcatcga aggagacctt gtggccgagc gactcgaagt cttccgtctt
    12961 gtcggggtcc acggcgaggt gcgcgccgcg ccgcatcagg tggttgactt tggcgcgctc
    13021 cgtcgtcatc ggcgtcctct tgtggaaggt gatctcgtcg attccgaggt ggcgcttgaa
    13081 gtcggcgaag aagccgatca gcggctcgcc aatggtgccg gttccgacga cgtgtacgat
    13141 cttgctcatg gccgtctctc ccgcatctca atttcggccg tggaggagat tccgcggacg
    13201 gggacgaccc gaagaatacg atgagacaac gatttcgcca acgcgcggcg gttccgtgag
    13261 cgcacggctc cacgcgttcc tcttccccgg cccaaccggg cagctcgaag ggctctggaa
    13321 aggcgcggaa aacggacacg cgggctccgc ggtgttcgcg catccccatc cggtctacgg
    13381 cggaacgctc cacagcaaag tcgtgttccg ggccgcgcgc actctctccc gcgccggata
    13441 cggagttctg cgcttcaatt tccgcggagt cgggctttcg gaggggcggt acgacgaggg
    13501 acggggagag acggcggact accgggccgc gctcgacgag gcggagcggc ggggcggcca
    13561 gccgatcgtc gccgggggct tctccttcgg ttcggcagtc ggcctgaagg cgatcgaggg
    13621 ggacccgcga gtcgttgcct tcatcggcgt cggcgtaccg ctcgcatccg atgtcggcgc
    13681 cgatctcccg ttcccgaaag tgccggctct cttcgtcgtc ggaaaagacg atgtcttcgg
    13741 tcctcccgcg cggctccgcg agtgggcggc cgggagaggc ctcgtcgtcg aggtggcg
//
LOCUS       DASWAV010000638         8152 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U2665
            k141_76818, whole genome shotgun sequence.
ACCESSION   DASWAV010000638 DASWAV010000000
VERSION     DASWAV010000638.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960316
            Sequence Read Archive: SRR10260007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 8152)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8152)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:47:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,929
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8152
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_76818"
                     /isolate="SMAG_U2665"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.36444 N 132.31889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1203)
                     /locus_tag="VF958_13275"
     CDS             complement(<1..1203)
                     /locus_tag="VF958_13275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002614985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HEX9150128.1"
                     /translation="MVVKLGDLLLKAKLVTPEQVDAALKSQRAEGGKFGEALIRLGAV
                     SETDITETLSQQFGVPSIDLAHFEIDPALIKVVPGEVARKYGVLPVNKTGATLTIAMS
                     DPTNVFAMDDIKFMTGYNVEPVVASEASLRRAIDKHYGTPRSVVLKERPKGSTTGSAP
                     SEGGIEDLMSSALTMDDMAGVNLSELNMDEITGIDDVDVVRGGEEGQEIDLIDLAKSS
                     ESAPIIKVSNLILIEALKAGASDIHVEPYEKEFRVRFRVDGILHNIMALPMRTRDPLI
                     SRLKIMAKLDISEKRLPQDGRIKIRLRVEERTRDLDLRVSVVPTHFGEKVVMRLLDKS
                     RLQLDMTQLGFDQEPLRRFKEAIDKPYGIVLVTGPTGSGKTNTLYSAIAQLNDPTVNI
                     LTAEDPIEF"
     gene            complement(1265..1999)
                     /locus_tag="VF958_13280"
     CDS             complement(1265..1999)
                     /locus_tag="VF958_13280"
                     /inference="COORDINATES: protein motif:HMM:NF022750.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3108 domain-containing protein"
                     /protein_id="HEX9150129.1"
                     /translation="MSPVRILFSASVLALALVPAGFAQPKLEGRPAFVGETLRFAMSI
                     LGAAGGELTLSAKETQLDGKPAYKFELSAISGEFLSKIFLVRDYLASWIDPRTFRSLR
                     FEKHTVEGKRVRDDLIEFDYEKKVAYRDGKPIAIEENTLDSLSSIYYIRLMDLESRQP
                     IPLTVVSRHLYPLTVVAQGRETVTTPAGTFKTIRVEPQGPEGLIGKGKTLILWLTDDE
                     KKMPVQLKSKLKVGTLTGKLKAVEKQ"
     gene            complement(2134..3093)
                     /gene="waaC"
                     /locus_tag="VF958_13285"
     CDS             complement(2134..3093)
                     /gene="waaC"
                     /locus_tag="VF958_13285"
                     /inference="COORDINATES: protein motif:HMM:TIGR02193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008920 - lipopolysaccharide
                     heptosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide heptosyltransferase I"
                     /protein_id="HEX9150130.1"
                     /translation="MKVLIVRLSSLGDVVHTLPLAANARRSGAQVYWVAEAAYRDLLE
                     ANPSIDRVFVADTKGWRRRPFSPRTFRQIAELRRDLSALAVDVTIDAQGLWKSALIAR
                     AAGAPVVGFCAGARREPTSAILGHRSVQPLAGGHVVDQNLSLAEAAGLTVFERRPDAR
                     FLLARPSLPAREFLEGQPRPFALYHPGARRPEKTWGEERFARLGDLLRTGSSLAPVVS
                     WGPGDESRARRLAALLPGARLLPLLDLPALAHVMAAASLVVAGDTGPLHLADAVGART
                     LALFGPTDPGRNGPYGQPASVVRYDSKTNAEEVAGKAIEVLAA"
     gene            complement(3090..3590)
                     /locus_tag="VF958_13290"
     CDS             complement(3090..3590)
                     /locus_tag="VF958_13290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002627532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylyltransferase/cytidyltransferase family
                     protein"
                     /protein_id="HEX9150131.1"
                     /translation="MSVAKRSSLEKIFSRKEVIRRALEERGAGRRIVFANGAFDLLHA
                     GHVRYLEAARAEGDWLVVAVNSDASVGRAKGMGRPVVPGSERAEIVAALGCVDAVVVF
                     EEDSPAALLQEMRPDVHAKGTDYSSASVPEREVVAGYGGRTVIVGDPKEHATTDLIER
                     IRKSPG"
     gene            complement(3587..4609)
                     /locus_tag="VF958_13295"
     CDS             complement(3587..4609)
                     /locus_tag="VF958_13295"
                     /inference="COORDINATES: protein motif:HMM:NF012515.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional ADP-heptose synthase"
                     /protein_id="HEX9150132.1"
                     /translation="MSLPVSKERLFALVSSFGGRRVLVAGDYVLDRFVFGHPKRISRE
                     APVLILRFWKEESLPGAAGNTAANVRALGGTPVPVGAVGADPAGASLKEIFRDRGIAT
                     DGLIEVAGYRTPTKTRVLAGGAHSIKQQVVRYDLEDHVPEVGPWRQLLGEKLAHAARD
                     VDAAVLSDYGYGAISPDAVAPLRAKLSAGVPLLVDSRHALGRYAGVDAATPNEEELEE
                     LAGGPVGEDPGDFAAAGDRLRRSIGCSTLLVTRGSRGMALFDGATKPTLIPVHGTDQV
                     ADVTGAGDTVLATFSLALASGASPLEAALLANFAGGVVVMKMGTATASAEELTDAIAS
                     DRTLTT"
     gene            4645..5229
                     /locus_tag="VF958_13300"
     CDS             4645..5229
                     /locus_tag="VF958_13300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020468984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HEX9150133.1"
                     /translation="MLDSQVLVLNRVFQAVHVTSVRKAFTLFYKGHVRAVLPDYSTYE
                     FENWCDIPVQPQDEVVLTPSMAIRVPRVVALKSFDRLPRQDVKFSRHNIYLRDGNRCQ
                     YCGHKFTSSELSLDHVIPLSRGGHSSWDNVVCACLSCNVKKGNRTPGEASMRLIGAPK
                     KPRWHPVGHFGANKFHPAWRNFLDIAYWNTELKS"
     gene            complement(5219..6214)
                     /gene="waaF"
                     /locus_tag="VF958_13305"
     CDS             complement(5219..6214)
                     /gene="waaF"
                     /locus_tag="VF958_13305"
                     /inference="COORDINATES: protein motif:HMM:TIGR02195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008920 - lipopolysaccharide
                     heptosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide heptosyltransferase II"
                     /protein_id="HEX9150134.1"
                     /translation="MSGWLVIAPNWIGDAVMSLPVLRALARASSGERLTVLARPGPAA
                     IYRAEGSAEEVRAVSGLAADSLATARSGFREAWLLPNSFRSALVPYLAAVRERIGYAT
                     DGRAALLTHPLPPPPRDRHQLRDYDPLLLSRGIEPDTRPPRLPIPPSAAERAGAALAG
                     ARLSSAGGRLLLLAPGAAFSWTKRWPPDRFGRLASELLQREWAPALAIGPGEQDLARK
                     VLDAAGVPLPVIGADLDPVELAAVAARARAVVANDSGAMHLSAAVGTPVVALFGPTDP
                     GRTGPVGAASIVLDRYMFCSPCYLKECPYGHECLREISVVAVASAVEKLAATVRT"
     gene            complement(6211..7380)
                     /locus_tag="VF958_13310"
     CDS             complement(6211..7380)
                     /locus_tag="VF958_13310"
                     /inference="COORDINATES: protein motif:HMM:NF015251.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HEX9150135.1"
                     /translation="MKAAQPDAVGAPAEASTAGAGRRRRKRRRSELRHRTELALFRIA
                     ATGLAVLPRSSGRRTLRGVTRLAFALLPARRRILSANLTAAFPEKTPGEIAAIGQRSL
                     DGFANALADFLETARLTREDLLARVTVAGEENLRSARARGRGVFLLSAHFGSWEIGAL
                     VAGLLGEPISPVARPLDNPLLEEELDSLRRHFGNRPISKRDAAKELLRAMSRNETVAI
                     LIDQNVLPREAVFVPFFDRPAATTPSLARIQLKTGAAVVPVFTWPRGEGAYRLEFERP
                     ILPEDFGGAEIPRDERVWRATARYMKVTEEAIRKDPAAWLWIHDRWKTKPPEDDQRPK
                     TRDQSNSGSRREESPEVIEGRASRRGNANAARDQASGRAGHREPLADRESEIPSK"
     gene            complement(7377..>8152)
                     /gene="lpxK"
                     /locus_tag="VF958_13315"
     CDS             complement(7377..>8152)
                     /gene="lpxK"
                     /locus_tag="VF958_13315"
                     /EC_number="2.7.1.130"
                     /inference="COORDINATES: protein motif:HMM:NF014645.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009029 - tetraacyldisaccharide 4'-kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="tetraacyldisaccharide 4'-kinase"
                     /protein_id="HEX9150136.1"
                     /translation="GPEEGGDEPVAIARRVPGVVVVVGEKRADAARAAIDRGADLILL
                     DDGFQHLGLRRDLDLLLVDASDPFGGGRLPPEGRLREPLSALSRADAVVFTRIDRGEP
                     PAGVVAELARRNPGAPVFTARIRSAGLWDERGSPVASSLLAARRFVAVCGIANPGRFA
                     QSIRELDLTAEEILTFPDHHAYRRRDLEAIRAAADERGGAWILTTEKDAVKLEGKTSL
                     PVVTLRIEVEVGEAGFFPFLFSRIAAVSPPSRRVSAPRS"
BASE COUNT         1299 a         2783 c         2767 g         1303 t
ORIGIN      
        1 gaactcgatc ggatcttccg ccgtgaggat gttgacggtc ggatcgttca actgggcgat
       61 tgcggaatac agcgtgttcg tcttcccgga accggtcgga ccggtgacga gcacgatccc
      121 gtacggcttg tcgatggcct ctttgaaacg gcgcagcggt tcctggtcga acccgagctg
      181 cgtcatgtcg agctgcagtc gcgacttgtc gagcagccgc atgacgacct tctcaccgaa
      241 gtgagtcggg acgaccgaga cgcgcaggtc gaggtcgcgc gtgcgttcct cgaccctcag
      301 ccggatcttg atgcggccgt cctgcggcag gcgcttctcg gaaatgtcga gcttcgccat
      361 gatcttcaga cgcgagatga ggggatcgcg ggtccgcatc ggaagagcca tgatgttgtg
      421 gaggatgccg tcgacgcgga agcggacccg gaactccttc tcgtacggct cgacgtggat
      481 gtcggaagct cccgctttca gcgcctcgat caggatgaga ttggatacct tgatgatcgg
      541 cgccgactcg gacgacttcg cgaggtcgat gaggtcgatt tcctggcctt cctccccgcc
      601 gcggacgacg tcgacgtcgt cgatgcccgt gatctcgtcc atgttgagct cggaaagatt
      661 gacgccggcc atatcgtcca tggtcagcgc cgagctcatg aggtcctcga tccccccttc
      721 gctcggcgcg ctgccggtcg tggaaccttt cggacgctcc ttcagcacga ccgaccgcgg
      781 cgtgccgtaa tgcttgtcga tcgcccggcg cagcgacgcc tcggacgcca cgaccggctc
      841 gacgttgtac ccggtcatga acttgatgtc gtccatcgcg aaaacgttcg tcgggtcgga
      901 catcgcgatc gtcagcgtgg caccggtctt gttcaccggg agcacgccgt acttgcgcgc
      961 gacctcgccc gggacgacct tgatcaacgc cggatcgatc tcgaagtgcg cgaggtcgat
     1021 cgacggaacg ccgaactgtt gcgagagcgt ctcggtgatg tcggtttcgg acacggcgcc
     1081 cagccggatc agggcctcgc cgaacttgcc gccctcggcc cgttgcgact tcaatgcggc
     1141 gtcgacctgt tcaggcgtga cgagcttcgc cttcagaagg agatcgccca gttttacgac
     1201 cattggaagc gatagagggt agagcttttg ggccgacctg tcaaaagttg ggtcaaattc
     1261 cagctcactg cttttcgacc gccttaagct tcccggtgag cgtccccacc ttcagtttcg
     1321 acttcagctg caccggcatc ttcttctcgt cgtcggtcag ccagaggatc agggtcttgc
     1381 cttttccgat caaaccctcc gggccctgcg gctcgacgcg gatcgtcttg aaggttcccg
     1441 ccggagtcgt gaccgtctcc cgcccctgcg cgacgaccgt cagcggataa aggtggcgcg
     1501 acacgacggt cagagggatc ggttgacggc tctcgaggtc catcaggcgg atgtagtaga
     1561 tcgacgagag cgagtcgagc gtgttctctt cgatcgcgat cggcttcccg tcgcgatacg
     1621 cgaccttctt ctcgtaatcg aactcgatca ggtcgtcgcg gacccgcttg ccttcgaccg
     1681 tgtgcttctc gaagcggagc gagcgaaacg tccgggggtc gatccaggag gcgagatagt
     1741 cgcgcacgag aaagatcttc gagagaaact cccctgagat cgccgacagc tcgaacttgt
     1801 acgccggttt tccgtcgagc tgcgtctcct tcgccgagag ggtgagctcc ccgcccgcgg
     1861 cgcccaggat cgacatggca aagcggagcg tctccccgac gaacgcggga cgaccctcga
     1921 gcttcggctg ggcgaagccg gcaggaacga gcgcgagcgc gaggacggac gcagagaaga
     1981 ggatgcgaac cgggctcaag cgcgggagag aacgaggaga atcaacaaat cgttcccggg
     2041 ggtccggtct ccacttgcca ctttgcctca caaagtggca agggcattca ttcgtagtca
     2101 cagatccggc gatcagtgag gacctgaccc cactcaggcc gcaagcacct cgatcgcctt
     2161 gccggcaact tcttccgcgt tcgtcttcga atcgtagcgc accaccgatg ccggctgccc
     2221 gtacggcccg ttgcgtccgg gatcagtcgg tccgaagagg gcgagcgtcc gagcgccgac
     2281 ggcgtcggcg aggtggagcg gcccggtgtc gccggcgacg acgagcgagg cggcggccat
     2341 gacgtgcgcg agcgccggga gatcgagcag cggcaggagg cgtgctccgg gaaggagcgc
     2401 cgcgagccgc ctggcgcggc tctcgtctcc gggtccccac gacacgaccg gcgcgaggct
     2461 gctgcccgtc cgcagaagat cgccgagcct cgcaaagcgc tcctctcccc acgtcttctc
     2521 gggacggcgc gcgccgggat ggtagagggc gaacggacgc ggctggcctt cgaggaattc
     2581 cctcgccggg agcgacggcc gggcgagaag gaaccgggcg tcggggcggc gctcgaagac
     2641 cgtcagaccg gccgcctcgg ccagcgacag gttctggtcg acgacgtgac ctccggcgag
     2701 cggctgaacc gaccggtgcc cgaggatcgc ggaggtcggc tccctccgtg cgcccgcgca
     2761 gaatccgacg accggcgcgc cggcggcccg ggcgatcagc gcggatttcc agagtccctg
     2821 cgcgtcgatc gtgacgtcga ccgcgagtgc ggagaggtcg cgccggagct ccgcgatctg
     2881 ccggaacgtg cgtggcgaaa acggtcggcg ccgccatcct ttcgtgtccg ccacgaagac
     2941 gcggtcgatc gagggattcg cctcgagcag atcgcggtag gcggcctcgg ccacccagta
     3001 cacctgcgcc ccggaacgtc gggcattcgc cgcgagcggc agcgtgtgga cgacgtcgcc
     3061 gagcgaggag aggcggacga tgaggacctt catccaggcg attttcggat ccgctcgatc
     3121 aggtcggtcg tggcgtgctc cttcggatcg cctacgatga cggtccggcc gccgtatccc
     3181 gccacgacct cgcgctcggg cacggacgcg ctcgagtagt ccgtcccctt cgcgtggacg
     3241 tcgggacgca tctcctggag gagcgccgcc ggcgaatcct cttcgaacac gacgaccgcg
     3301 tcgacgcagc cgagcgccgc gacgatctcg gcgcgttcgg atcccggcac cacggggcgc
     3361 cccatcccct tggcgcgccc gacggaggcg tcggagttca cggcgacgac gagccagtct
     3421 ccttcggctc gcgcggcctc gagatacctc acgtgtccgg cgtggagaag atcgaacgcc
     3481 ccgttggcga aaacgatccg gcgacctgcc cctctctctt ccaaggcccg gcggatgact
     3541 tccttccggg agaagatctt ttccagcgac gatcgcttcg cgacgctcac gtcgtgagcg
     3601 tccggtcgga cgcgatcgca tccgtgagct cttccgccga cgccgtcgcc gtgcccatct
     3661 tcatcacgac gacgccaccc gcgaagttgg cgaggagcgc ggcttcgagc ggcgatgcgc
     3721 ccgacgcgag cgcgagcgag aacgtggcga gaaccgtgtc tcccgctccg gtgacgtcgg
     3781 cgacctgatc ggtgccgtgg acggggatga gagtcggttt cgttgcgccg tcgaagagcg
     3841 ccatgccacg ggaaccgcgg gtgacgagca aagtcgaaca gccgatgctg cgccgcagcc
     3901 gatcgccggc cgccgcgaag tcccccggat cttcgccgac cggtcccccc gccaactctt
     3961 cgagctcctc ttcgttcggc gttgccgcat cgacccccgc gtagcggccg agcgcgtgac
     4021 gcgagtcgac gaggagggga actcccgcag acagctttgc gcggagcggc gcgacggcgt
     4081 cgggcgagat cgcgccgtaa ccgtagtcgg agagcacggc ggcgtccacg tctcgagcgg
     4141 cgtgcgcgag cttctcgccg agaagttggc gccacggacc aacctccggc acgtggtctt
     4201 cgaggtcgta gcgcacgacc tgctgcttga tcgagtgtgc gccgccggcg agcacgcgcg
     4261 tcttcgtcgg agtccgataa ccggcaacct cgatcaagcc gtcggttgca atgccccggt
     4321 ctcgaaaaat ctccttcagg ctggctcccg ccggatcggc gccgactgct ccgacgggaa
     4381 cgggcgtgcc cccgagggcg cgcacgttcg cggccgtgtt gccggcggcg ccggggaggc
     4441 tctcctcctt ccagaagcgc aggatcaaga caggcgcctc gcggctgatc ctcttgggat
     4501 gcccgaacac gaagcggtcg agcacgtaat ctccggcgac gagaacccgc ctgccgccga
     4561 aggacgaaac gagagcgaac aggcgctcct tcgagaccgg aaggctcatc ggcgctatat
     4621 aatgacgccg ttttcggtca accgatgctc gactctcagg ttctcgtact caaccgcgtc
     4681 tttcaggcgg tccatgtgac gtccgtgcgg aaggcgttca cgctcttcta caagggtcac
     4741 gtccgcgccg tcctcccgga ctactcgacc tacgagttcg agaactggtg cgacattccc
     4801 gtgcagccgc aggacgaggt cgtgttgacg ccgtcgatgg cgatccgggt cccgcgtgtc
     4861 gtggcattga agagcttcga ccggttgccg cggcaggacg tgaagttttc ccgccacaac
     4921 atttacctgc gcgatgggaa tcgctgccag tactgcggcc acaagttcac ttcttccgag
     4981 ctgtcgctcg accacgtgat cccgctctcc cgtggcgggc actcctcctg ggacaacgtc
     5041 gtctgcgcct gcctgtcgtg caacgtgaag aaggggaatc gcacgccggg ggaggcctcg
     5101 atgcggctga tcggcgcccc gaagaagccg cgctggcatc cggtcggcca cttcggagcg
     5161 aacaagtttc accctgcctg gcgcaatttc ctcgatatcg cgtactggaa caccgagctc
     5221 aagtcctgac cgtcgcggcg agcttctcga ccgccgacgc caccgctacg accgaaatct
     5281 cccgcaggca ttcgtgcccg tagggacact ccttcaggta gcacggcgaa cagaacatgt
     5341 accggtcgag gacgatcgac gccgcgccga ccggcccggt ccttcccgga tccgtcggcc
     5401 cgaagagggc caccaccgga gttcccaccg cagcggagag gtgcatcgcg cccgagtcgt
     5461 tcgcgaccac cgctcgggcg cgggccgcga cggccgcgag ctcgaccggg tcgaggtcgg
     5521 cgccgatgac ggggagcggc actccggcgg cgtcgagaac cttccgcgcg aggtcctgtt
     5581 cgcccgggcc gatcgcgagg gcgggcgccc actctctctg gagtagctcc gaggcgagcc
     5641 gcccgaagcg gtccggcggc caccgcttcg tccacgagaa cgcagcaccg ggcgccagga
     5701 gcaggagcct cccgccggcc gaggaaagtc gggcgcccgc gagcgccgcc ccggcgcgct
     5761 cggccgcgga cggcgggatc ggaagccggg gcggtcgggt gtcgggctcg atgccgcgcg
     5821 aaaggaggag ggggtcgtag tcgcgcagct ggtgacggtc gcgcggcggc ggaggaagcg
     5881 ggtgggtcag gagcgcggcc cggccgtcgg tcgcgtagcc gatccgctcc cggaccgcgg
     5941 cgaggtaggg cacgagcgcg gaacggaacg agttgggaag gagccaggcc tctcggaaac
     6001 cggagcgtgc ggtcgcgagc gaatctgcgg cgaggccgga gacggcgcgg acttcctccg
     6061 cggaaccctc cgcccgatag atcgccgccg gcccgggccg cgcgagcacc gtgagacgct
     6121 ctccggacga agctcgcgcg agcgccctca ggaccggcaa cgacatgacc gcatcgccga
     6181 tccagttcgg tgcgatgacc agccatccgc tcacttgctc gggatctcgc tttcgcgatc
     6241 ggcgagcggc tcgcgatgcc ccgctcgacc gctcgcctga tccctcgccg cgttcgcgtt
     6301 cccgcggcgc gaggctcttc cctcgattac ttcgggagac tcctcgcgcc gcgacccgga
     6361 gttgctctgg tctctggtct tcggtctttg gtcatcctct ggcggcttcg tcttccaccg
     6421 gtcgtgaatc cagagccacg ccgcgggatc tttccggatc gcctcctcgg tgaccttcat
     6481 gtagcgggcg gtcgcacgcc acacccgctc gtcgcggggg atctccgccc ctccgaagtc
     6541 ttccgggagt atcggccgct cgaactcgag gcggtatgcg ccctcccccc ggggccacgt
     6601 gaagaccggg acgaccgcgg cgccggtctt cagctggatc cgggcgagcg agggggtcgt
     6661 cgccgccggc cggtcgaaga aagggacgaa caccgcctct cgcggcagga cgttctggtc
     6721 gatcaggatt gcgaccgtct cgttgcggct catcgcgcgc aggagctcct tcgcggcgtc
     6781 cctcttcgag atcggtcggt tcccgaagtg gcgccgcaga gagtcgagct cttcctcgag
     6841 cagagggttg tcgagcggcc gcgcgacggg cgagatcggc tcgccgagaa gtcccgcgac
     6901 gagcgcgccg atctcccagc ttccgaagtg cgcggacagc agaaagaccc ctcggccgcg
     6961 cgcgcgggcg gaccggagat tttcctcgcc cgcgaccgtc acgcgcgcga gcaggtcttc
     7021 ccgagtcaag cgggctgtct cgaggaaatc cgcgagcgcg ttcgcgaacc cgtcgagcga
     7081 tcgttgcccg atcgccgcga tctcccccgg cgtcttctcg gggaacgccg cagtcaggtt
     7141 cgccgagagg atccgtcgcc gggcgggaag gagcgcgaac gcgagcctcg tcactccccg
     7201 gagagtcctc cgtccgctcg accgtggcag caccgcgaga cccgttgcgg caatccggaa
     7261 gagcgcgagc tccgtccggt gacgcagctc cgatcggcgc cgcttccgcc gccgtctccc
     7321 ggctccggcg gtcgaggcct cggccggcgc gcccacggcg tccggctgcg cggccttcat
     7381 gaccggggag ccgagacccg gcgggacggg ggcgaaaccg ctgcgattcg cgagaacaag
     7441 aacgggaaga agcccgcctc gccgacctcg acttcgatcc ggagcgtgac gaccggtagc
     7501 gacgtcttcc cctcgagctt caccgcgtcc ttctcggtgg tcaggatcca ggcgccgccc
     7561 ctctcgtccg ccgccgcgcg gatcgcttcg aggtcgcggc gccggtaggc atggtggtcg
     7621 gggaaggtga ggatctcctc ggccgtgagg tcgagctccc ggatcgactg cgcgaaccgg
     7681 ccggggttcg cgattccgca gaccgcgacg aagcggcgcg ccgccagcag ggacgacgcg
     7741 acgggcgatc cccgttcgtc ccagaggccg gcggaccgaa tccgggccgt gaacaccggg
     7801 gcccctggat tccggcgcgc gagctcggcg acgaccccgg cgggaggttc cccgcgatcg
     7861 atccgggtaa ataccaccgc gtcagcgcgg gagagggcgg agaggggctc gcggagccga
     7921 ccctcgggag gaagccttcc gccgccgaac ggatcggacg cgtcgacgag cagcaggtcc
     7981 aggtcgcgcc gcaggccgag gtgctggaag ccgtcgtcga gcaggatcag gtcagcgccg
     8041 cggtcgatcg cggcgcgtgc ggcgtccgcc cgcttctctc cgacgacgac gacgacgccc
     8101 ggtactctcc gcgcgatcgc gaccggttcg tcccccccct cctccggtcc ga
//
LOCUS       DASWAV010000639         5340 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U2665
            k141_200132, whole genome shotgun sequence.
ACCESSION   DASWAV010000639 DASWAV010000000
VERSION     DASWAV010000639.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960316
            Sequence Read Archive: SRR10260007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 5340)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5340)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:47:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,929
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5340
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_200132"
                     /isolate="SMAG_U2665"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.36444 N 132.31889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..985)
                     /locus_tag="VF958_13320"
     CDS             complement(<1..985)
                     /locus_tag="VF958_13320"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF014564.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HEX9150137.1"
                     /translation="MADTTSFPKHKVLNWPAVPIPTPDEVYAGLTDQAKEYPLSLEAQ
                     LRQSENFKKPECLTTVRVLDLSKGMIIGNWCSSMLSELGADTIKCEPPEGDPLRWQTP
                     FGRKEYMFKDIETGEPIGLHFLHEMRNKKSVTLDLEKPEGRELLKKLVVHFDVLIENY
                     PPGQFDAWGIGYRQLKEINPRLVYAWVGQKGQWGPSKDKPGNLDPTAQASCGFVHGTG
                     LPKEFGGRPTRSAMWMADQVGGTSTAMGILAALLYRDKVSGKGQFIEGTAAEAVIRIL
                     DYNWAWYGMDGSIRPRYGNWDLAINIYAVNPCKDGYFMVGGGHDRLWYRIWR"
     gene            complement(1335..2519)
                     /locus_tag="VF958_13325"
     CDS             complement(1335..2519)
                     /locus_tag="VF958_13325"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OFA family MFS transporter"
                     /protein_id="HEX9150138.1"
                     /translation="MNLALGSLYAWSVFVAPLEKEFGWKRADTSQVFTVAVVVFAISF
                     ILAGRLQDKKGPFFISLVGCILLTIGFLLCAKTNSLGYLIFCFGVLVGIGNGFGYATP
                     IPVMAKWFPDKRGLAVGLAVAGYGGGSAIFGPLALKWLIPTYGWRTTFVVLGVVFFFM
                     TLVGAFLLKNPPAGYRPPGWAPAPAAKAAATTHEFTPGEMLRTPTFYFMWIAYALGAS
                     AGLMVISQLIPFAKSVGVAAAALAAISLVIAAAGNASGRIFSGWMSDALGRLNVLRLM
                     IGISAVGMFILPHTGTQMEVPLFLYLVVFVVYWCYGTQLSVNASTTSDFWGTKNAGIN
                     YGLLFTSWGVAGIIGPRIGGKLFDKFGNYKAAFYTAAVLAIVALIFEMMAKRPVPPRE
                     TA"
     gene            complement(3028..4371)
                     /locus_tag="VF958_13330"
     CDS             complement(3028..4371)
                     /locus_tag="VF958_13330"
                     /EC_number="2.3.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010933494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046912 - acyltransferase activity, acyl
                     groups converted into alkyl on transfer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate synthase"
                     /protein_id="HEX9150139.1"
                     /translation="MQALEAEKKQDPGVAPAGKKDALTVIDQRTGKSYELPIADGTIR
                     AIDLRQIKTDPQDFGLMTYDPAFMNTASCRSRITFIDGDKGILRYRGYPIEQLAEESS
                     YLEVAYLLLNGELPTKEQLDQWVHHITFHTMPQESLKKFMDGFNYDAHPMGMLIATVA
                     AMSTFYPDAHNIHDPVSRQTQTWRLIAKMPTLAALAYRHSMGMPYVHPDNDLSYTENF
                     LNMLFKIPSASWRPDPVLVRALDVLFILHADHEQNCGTHAMRGIGSSHTDPYSAVAGA
                     AAALYGPLHGGANEQVLKMLTEINSISNVPAFIKEVKEGRGEKRLMGFGHRVYKNYDP
                     RAKIIKRIADEVFQVTGRNPLLDIALELERIALQDEYFVKRKLYPNVDFYSGLIYQAI
                     GFPTSVFTVLFAIPRTAGWMAQWQEMLADPEQKIARPRQIYLGSAERNYVPIEKR"
     gene            complement(4529..>5340)
                     /locus_tag="VF958_13335"
     CDS             complement(4529..>5340)
                     /locus_tag="VF958_13335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9150140.1"
                     /translation="KDSRSPVSPTKVAQTCNHCHGDAALMSAHKLPSDPYEKWSRSVH
                     YRARVEKGDLSAPTCNSCHGNHGAAPPQVGSVANVCGTCHVTFADQFKLSPHWEAFKE
                     MELPGCVTCHENHEIVKPSEAFLAEGDQGKCSSCHEAGSAGARAAAAMRADLVGLDGE
                     IRRAGELVNRAAEAGMEVSKVRFDLVSATNALTKARSQVHLFRPSAVHVAVAEGLTVA
                     RKAAAGGESKLKERDFRQRGLFVSLALILLSIAALVVRIRERDRSTPHSGE"
BASE COUNT          971 a         1683 c         1721 g          965 t
ORIGIN      
        1 tcctccagat ccggtaccag aggcggtcgt gtccgccacc caccatgaag tagccgtcct
       61 tgcagggatt gacggcgtag atgttgattg cgaggtccca gttgccgtag cgaggccgga
      121 tgctcccgtc catcccgtac cacgcccagt tgtagtccag gatccgaatg acggcctccg
      181 ccgccgtccc ttcgatgaac tggcccttgc cggaaacctt gtcacgatag aggagcgcgg
      241 ccaggattcc catggccgtc gaggtccctc cgacctggtc cgccatccac atcgcggagc
      301 gcgtgggccg cccgccgaac tccttcggga ggcccgtgcc gtgcacgaaa ccgcaggacg
      361 cctgcgccgt cgggtcgagg ttgcccggtt tgtccttgga cggtccccac tggcctttct
      421 gtccgaccca ggcgtagacg agacgcgggt tgatttcctt cagttgccgg taaccgatcc
      481 cccacgcgtc gaactgcccc ggcggatagt tttcgatcaa gacgtcgaag tggacgacca
      541 gctttttcag cagctcgcgg ccctccggct tctcgagatc gagcgtgacg gacttcttgt
      601 ttctcatctc gtggaggaag tggaggccga tcggctcgcc ggtctcgatg tccttgaaca
      661 tgtattcctt gcggccgaag ggcgtctgcc agcgcagggg atcgccttcc ggcggctcgc
      721 acttgatcgt gtcggcgccc agttccgaga gcatcgagga gcaccagttg ccgatgatca
      781 tccccttgct caagtcgagg acccgcaccg tggtcaggca ctccggcttc ttgaagttct
      841 cgctctgtct gagctgtgcc tccagagaaa ggggatactc cttggcctga tcggtcagcc
      901 ccgcgtagac ctcgtccggc gtcgggatcg ggaccgccgg ccagttcaga accttgtgtt
      961 tcggaaacga cgtcgtgtca gccatccgga cctcctgtta gtcgaaaaaa gccgattccc
     1021 cgcggagagg gcgcggtctc cctgcggggg ccggcccgga aacgaactcg cagatgccag
     1081 tccctgcgct cgggcgccct cccggaaatg cgccgttggc aagagccccg gctgccgtct
     1141 cgagcaagtc ggtctggagc ggctcgtcca tgccggcgat gctccgcgcc agtaatgaac
     1201 cgccataact cgatccgacc gacttgcctt cgacgcgccg atgagcgaca gggccccggc
     1261 gtccagaacg agctcgtctg aatcattccg ctcgaaagat ggttcgtcgt cacgaactgt
     1321 agtaccccgt tccgctacgc cgtctccctc ggcggcaccg gccgcttcgc catcatctcg
     1381 aagatgagcg cgacgattgc cagaaccgca gcggtgtaga aggccgcttt gtaattcccg
     1441 aacttgtcga agagcttgcc gccgatccgg ggcccgatga tgccggccac gccccaggaa
     1501 gtgaacagca ggccgtagtt gatgccggcg tttttcgtgc cccagaagtc ggaggttgtg
     1561 gaggcattca ccgagagctg agtgccatag caccagtaga cgacgaagac caccaggtac
     1621 aggaaaagcg ggacctccat ctgggtgccg gtgtgaggca ggatgaacat gcccactgcc
     1681 gagatcccga tcatcaggcg caggacgttc agccggccga gcgcgtcgga catccagccc
     1741 gagaagatgc gaccggacgc attgcccgcg gcggcgatga ccaggctaat ggccgcgagc
     1801 gccgcggccg cgacgccgac ggacttggcg aacgggatga gctggctgat gaccatgagc
     1861 cccgctgacg cacccagcgc gtaagcaatc cacatgaaat agaacgtggg ggtgcgcaac
     1921 atctcccccg gagtgaactc gtgcgtcgtc gccgccgcct tggcggcagg ggcaggcgcc
     1981 cagcccggcg ggcggtaccc agccggaggg ttcttcagca ggaaggctcc gaccagggtc
     2041 atgaagaaga agacgacacc gagcacgacg aaggtggtgc gccagccgta cgtcgggatg
     2101 agccacttga gcgccaaggg cccgaagatg gccgaaccgc cgccgtagcc ggccacggcc
     2161 aggccgaccg ccagaccgcg cttgtccggg aaccacttcg ccatgaccgg aatgggcgtc
     2221 gcgtaaccga acccgttgcc gatgccgacc agcacgccga agcagaagat gaggtacccc
     2281 aggctgttgg tcttggcgca gagcaggaag ccgatcgtca ggagaatgca acccaccagg
     2341 gatatgaaga agggaccctt cttgtcctgc aatcgtcccg cgaggatgaa gctgatggcg
     2401 aagacgacga ccgcaacggt aaatacttgc gacgtgtctg cccgcttcca gccgaactcc
     2461 ttttccaggg gggcgacgaa gacgctccag gcgtagagcg aaccgagcgc gagattcatg
     2521 agcattccgc ccacgacacg ccaccatcga ctgagtccca cttcgacgtg gggtacggca
     2581 acggcccccg gggttgttcc agcggtgctc gccatttctc aacctcctcg tttgggttcc
     2641 tgaccttctc tctagggcct cctccgagtc cgttcgggac ttttggtcga ttcgctttca
     2701 gtcacggaag gtttgcattc agatcgcgga ctggtccgcg gcgaagccag attggggctc
     2761 gtacttggaa accagttcgc gaaatcaagc ctatgccgaa gaccgggcgc gaggcaatat
     2821 cggttcatcc ccggttcatc caggaaaagt tcggggaagg aaaaggccga gaaaacaact
     2881 gacgcgccag aatgtcgtgg gaaggtcgag tgaacggagc cgggacgggt tgggcgacga
     2941 agtggccgaa acctgaacaa tctcgtcccg ggaaggggtg tcgccctccg ggcttgccgg
     3001 gagggctgca tccggccgaa gagcgcctca gcgcttttcg atcgggacgt agttgcgctc
     3061 ggcagagccg agatagatct gccgcgggcg cgcgatcttc tgctcgggat cagccaacat
     3121 ctcctgccac tgtgccatcc agccggcggt acggggaatc gcgaaaagca ccgtaaagac
     3181 cgaggtcggg aagccgatcg cctggtaaat caggcccgaa tagaagtcaa cgttcgggta
     3241 gagcttgcgc ttgacgaagt attcgtcctg gagggcgatc ctctcgagct ccagagcgat
     3301 gtcgaggagc ggattgcgcc ccgtgacctg gaagacctcg tcggcgattc gcttgatgat
     3361 cttggcccgg gggtcatagt tcttgtagac gcggtggccg aagcccatca gacgcttctc
     3421 gcctctgcct tctttgactt ccttgatgaa tgcgggaacg ttcgagatcg agttgatctc
     3481 cgtgagcatc ttcaggacct gttcgttggc cccgccgtgc aacggcccgt agagcgcagc
     3541 cgccgcaccc gcgacggccg aataggggtc ggtgtgggag gagccgattc cccgcatcgc
     3601 gtgggtgccg cagttctgct cgtggtccgc atggaggatg aagaggacgt cgagcgcgcg
     3661 caccaggacg gggtcggggc gccagctcgc cgacgggatc ttgaacagca tgttcaggaa
     3721 gttctcggtg taggagaggt cgttgtccgg atgcacgtag ggcatcccca tgctgtgccg
     3781 gtaggccagg gcggcgagcg tcggcatctt ggcgatcagg cgccaggtct gagtctgccg
     3841 cgagaccgga tcgtggatgt tgtgggcgtc cgggtagaac gtcgacatgg cggcaacggt
     3901 cgcgatcagc atccccatcg ggtgcgcgtc gtaattgaag ccgtccatga acttcttcaa
     3961 cgactcctgg ggcatcgtgt ggaaggtgat gtggtgcacc cattggtcga gctgctcctt
     4021 cgtcggcagc tccccgttca ggagcaggta ggcgacctcg aggtaactgg actcctcggc
     4081 gagctgctcg atcgggtagc cccggtagcg aaggatcccc ttgtccccgt cgatgaacgt
     4141 gatgcgactc cggcacgagg ccgtgttcat gaatgcgggg tcataggtca tcagcccgaa
     4201 gtcctgcggg tcggtcttaa tctggcgcag atcgatcgcc cgaatcgtcc cgtcggcgat
     4261 cgggagctcg taggacttcc ccgttctctg gtcgatcacc gtcagggcat ccttcttccc
     4321 cgccggggcg acgccggggt cctgtttctt ttctgcttcc aaggcctgca tcgataactc
     4381 ctctggacgg gtcgagtgag gcggattata tgggcgcgaa aaaaagggtt cgctgatgcg
     4441 ttttcatcag gactcgcagg cgaccgatcc ccggctcgag tccgaatcgg gcagacggcc
     4501 tgggatgccc gatccgggaa ccatgcggct actccccgga atgcggcgtc gagcggtcac
     4561 gctcccgaat ccggacgacc agcgccgcga tcgagagcag gatcagggcg agtgagacga
     4621 acagcccccg ctgccggaag tcgcgctcct tcaacttcga ctcgccgccc gccgccgcct
     4681 tgcgtgccac cgtcaaccct tccgcgaccg cgacgtgcac cgcggaggga cggaagaggt
     4741 ggacctggga gcgggctttc gtcagcgcgt tcgtcgccga aacgaggtcg aaccgaacct
     4801 tcgagacctc catccccgcc tcggcggcgc ggtttacgag ttcgcctgcc cgacggattt
     4861 cgccgtcgag gccgacgagg tccgcccgca tcgcggcggc cgcgcgagcg cccgcggagc
     4921 cggcctcgtg gcaactcgag cacttgccct ggtccccctc tgcaagaaac gcctcgctcg
     4981 gcttcacgat ctcgtggttc tcgtgacagg tgacgcagcc cggcagctcc atctccttga
     5041 aggcttccca gtgcggcgag agcttgaact gatcggcaaa ggtcacgtgg cacgtcccgc
     5101 acacgttcgc gacggagccc acctgcggcg gcgctgcgcc gtgattcccg tggcaggaat
     5161 tgcaggtcgg cgcggagagg tcaccttttt cgacccgcgc gcggtagtga acgcttcgcg
     5221 accacttctc gtaagggtcg gacggcaact tgtgcgccga catgagagcc gcgtcgccgt
     5281 ggcagtggtt acacgtctgg gccaccttcg tcggcgaaac cggagaccgc gagtccttga
//
LOCUS       DASWAV010000640         2250 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U2665
            k141_719198, whole genome shotgun sequence.
ACCESSION   DASWAV010000640 DASWAV010000000
VERSION     DASWAV010000640.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960316
            Sequence Read Archive: SRR10260007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2250)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2250)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:47:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,929
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2250
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_719198"
                     /isolate="SMAG_U2665"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.36444 N 132.31889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            136..1299
                     /locus_tag="VF958_13340"
     CDS             136..1299
                     /locus_tag="VF958_13340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9150141.1"
                     /translation="MLTAVLLLVGVLGLLPGDSAAQDLPPLPRGRGITRPLPPIPQRA
                     RTPPPARPAPTPPRPGAYVEEARVERVVIDAHVTYDDGEPIRDLVAADFVVRVDGKRV
                     PLESVEWVAASEPESAAVKPGEITWGETPSLPEPVLAYPPGRVIVLFYQTNYEPVRST
                     GFLRMAQYTDHFLETLLETDRVAVVSFDSHLKLRQDFTNSRQKIRDAMLSCLKTAEPG
                     RIEPGPFPSLARNFDYAAAKKAVTPERGLDLVARALEPIPGGKTMLFFGWGLGTIGGL
                     TGPIPKEIKEYHRALQSLSAARVNIFTLDITDADYHTLEGRLVGVSDATGGTYQKTHL
                     FPQAALDRVTRAISGRYVIVFVKPNLPRGAHGVEVTLAGKRKGNVSARSYYED"
     gene            complement(1419..>2250)
                     /locus_tag="VF958_13345"
     CDS             complement(1419..>2250)
                     /locus_tag="VF958_13345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9150142.1"
                     /translation="HDFRRAKDLRVTVSFPGQGGPFPVIVFSHGAGGNGNYPYPLTRF
                     WTSHGYVCIHPTHADSVSLGGGVSESGPPAWENRARDLSFVIDSLDELVAKVPALAGK
                     MDARRIGVAGYSYGGHSAQLLGGATLEAAGGGEPRTLSDPRPFAFLLLSGPGAGAREL
                     TERSWNGWTRPMMAVTGSLDLVEGERSPMWHLDPFLRSPAGDKYALYLEGAVHRSFTG
                     RQLELSDTRPEREIFRWICAETLAFWDLYLKNAAAAKPWLEPERVSELSGGRAKLEKR
                     "
BASE COUNT          379 a          799 c          711 g          361 t
ORIGIN      
        1 gaaatcacgc gcagagtcgt cgaacactgc ggccgctagc gtggcctcga gctatcttca
       61 gggtgagcga agacgtccca cgcgcgcacc ctgccgccct tgtacccggg tccgcgcgac
      121 tgtagcctct actcggtgct cacggcggtt cttctcctcg tcggcgtgct cgggctcctt
      181 ccgggcgact ccgccgctca ggatctcccg ccgcttccgc gcggccgcgg aatcacgagg
      241 ccccttcctc cgattcccca gcgggcgcgc acgccgcccc cggcacgacc cgctcccact
      301 ccgccgcggc cgggggccta cgtggaagag gcgcgcgtcg aacgcgtcgt catcgacgcg
      361 cacgtcacgt acgacgacgg cgagccgatt cgcgacctcg tcgccgccga cttcgtcgtg
      421 cgggtcgacg gcaagcgcgt gccgctcgaa tcggtcgagt gggtggcggc gagcgagccg
      481 gagtccgcgg ccgtcaagcc cggagagatc acgtggggag agacgccgtc gctgccggag
      541 ccggtcctcg cctacccgcc tgggcgcgtg atcgtcctct tctaccagac gaactacgag
      601 cccgtgcgca gcacgggctt cctgcggatg gcgcagtaca ccgaccattt cctcgagacg
      661 cttctcgaga ccgaccgggt tgcggtcgtg tctttcgact cgcacctgaa gctccggcag
      721 gacttcacga acagccggca aaagatccgc gacgcaatgt tgtcgtgtct caagaccgcg
      781 gaacccggcc ggatcgagcc cgggccgttc ccgtccctcg cgcggaactt cgattacgcc
      841 gcggcgaaga aggccgtcac gcccgagcgc ggcctcgacc tcgtcgcacg ggccctcgag
      901 ccgattcccg gcggcaagac gatgctgttc ttcggctggg gcctcggaac gatcggaggt
      961 ctcaccgggc cgatcccgaa agagatcaag gagtaccatc gcgcgctgca gtcgctttcc
     1021 gccgcgcgtg tcaacatctt caccctcgac atcaccgacg ccgactacca cacgctcgaa
     1081 ggtcgtctcg tcggcgtgtc ggacgcgacc ggcggcacct accagaaaac ccacctcttc
     1141 ccgcaggcgg ctctcgaccg cgtgacgcga gcgatctccg gccgctacgt gatcgtcttc
     1201 gtcaagccga acctgccgcg aggcgcccac ggcgtggaag tcaccctcgc cgggaaacgg
     1261 aaagggaacg tctcggcgcg cagctactac gaggactaga gggtcaggtc tccacttgcc
     1321 acattggggg tcaggtctcc acttgccaca tgcggcgcat ggggcaggac caccttctcg
     1381 aaacgaatat cgatgcgttt tgtgaagatc tgatcccgct atcttttttc caacttcgct
     1441 cttcccccgc tgagctccga gacgcgctcg ggttccagcc acggctttgc cgcggcagcg
     1501 tttttcagat agagatccca gaacgcgagc gtttccgcgc agatccaccg gaagatctcg
     1561 cgctccggac gcgtatccga caactctagc tggcggccag tgaacgagcg gtgcaccgca
     1621 ccctcgaggt agagcgcgta cttgtccccg gcgggactgc gcaagaacgg atcgaggtgc
     1681 cacatcggac tccgttcgcc ctcgacgaga tcgagcgatc cggtgacggc catcatcggc
     1741 ctcgtccagc cgttccagga gcgctcggtc agttcgcgcg cgccggcgcc gggtccggac
     1801 agcaggagaa aggcgaacgg tcgaggatcg gacaaagtcc gcggctctcc ccctccggcg
     1861 gcctcgagcg tcgcgccgcc gaggagctgc gcgctgtgac ctccgtacga gtagccggcg
     1921 acaccgatcc gccgcgcgtc catcttcccg gcgagcgccg gcaccttcgc gacgagctca
     1981 tcgagagagt cgatgacgaa cgagagatcg cgggcgcggt tctcccaggc gggcggcccg
     2041 ctctccgaaa ctccaccccc gagcgaaacc gagtcggcgt gcgtcgggtg gatgcagacg
     2101 taaccgtggc tcgtccagaa gcgggtcagc ggatacggat aattgccgtt gcctccggcc
     2161 ccgtgcgaga acacgatcac gggaaacggg ccgccctggc cgggaaagct gaccgttacc
     2221 cggaggtctt tcgcgcgccg aaaatcgtgc
//
LOCUS       DASWAV010000641         6550 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U2665
            k141_117183, whole genome shotgun sequence.
ACCESSION   DASWAV010000641 DASWAV010000000
VERSION     DASWAV010000641.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960316
            Sequence Read Archive: SRR10260007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 6550)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6550)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:47:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,929
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6550
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_117183"
                     /isolate="SMAG_U2665"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.36444 N 132.31889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..640)
                     /locus_tag="VF958_13350"
     CDS             complement(<1..640)
                     /locus_tag="VF958_13350"
                     /inference="COORDINATES: protein motif:HMM:NF012581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proton-conducting transporter membrane subunit"
                     /protein_id="HEX9150143.1"
                     /translation="MTPSFLAFGPNDLAAAAPEIALAVGGCLAVLLDAFAPSARRWLA
                     TLSLAAIAASTWFLVAAPAGLSFAGRYETSDLTVAAGLFASATAAVAILVAKPYLQRT
                     GEEKGEFYALLVWGHLGVLLMIRGLDLLVVFIGLETLSLSFYVLAAFFRPIQASSEAG
                     LKYFLTGAFASAFTLYGIALLFGKAGSVRVAALSRPDLAADPLVAFALLLLLV"
     gene            complement(637..2190)
                     /locus_tag="VF958_13355"
     CDS             complement(637..2190)
                     /locus_tag="VF958_13355"
                     /EC_number="1.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020674788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HEX9150144.1"
                     /translation="MPALFDQHLLTVLVFLPTVAAALLLLFPESRARGAKTFALAASI
                     IELALSLPLWSRFDLASGAFQFVEKAEWVPALGISYTVGIDGISLLLVLLTTVLTPVS
                     LLYSITHVSKSVRAFSMAFLVLETGVVGTLVALDLALFYVFWEVMLVPMYFIIGIWGG
                     GRRIYAAMKFFLFTMAGSLLMFLAILYLAIRHRAVTGGWSFLLADLARLTYPTMAIQT
                     VLFLAFALAFAIKVPVFPLHTWLPDAHTEAPTAGSIILAGILLKLGVYGYLRFALPLF
                     PDAALRYAPWLGVLGVIGVIYGACVAYAQSDMKRLVAYSSVSHLGLVVIGIGAFTSTA
                     MTGSVLQMINHGLSTGALFLLVGVLYERTHTREIADYGGIAGVVPVTTALFLVATFSS
                     IGLPGLNGFVGEFLILLGTWTSNHRWWAIAAATGVILSAVYMLALVQRVFWNPLTREE
                     NRRLPEIRPSELVAAAALVVLMVWIGVRPNDFLDRLRPSVEEVRRSVATRKTAALPPS
                     ARPGLEVPR"
     gene            complement(2192..4129)
                     /gene="nuoL"
                     /locus_tag="VF958_13360"
     CDS             complement(2192..4129)
                     /gene="nuoL"
                     /locus_tag="VF958_13360"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012471157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HEX9150145.1"
                     /translation="MTLALWVIPALPLAGFLFCLLLGRRLGKSFVTAAGVGSVGLATL
                     AAWWRLIPYFAGGGTAGVERIAPWIAAGNFSVDISLRLDPLSGLMVSFVTFVGFLIHV
                     YSIGYMRHDETDAGYARFFAYMNLFLFSMLTLVLANNFLLMFVGWEGVGLCSYLLIGY
                     YYEKDFAAAAGKKAFVVNRIGDFGFLLGMFGVFSLFGTLDFDGVFPRAAADPGRFAPG
                     LTVVCLLLFLGAMGKSAQVPLHVWLPDAMAGPTPVSALIHAATMVTAGVYMVTRCNVL
                     FRLAPEAMSVVAIIGGVTAIFAAVIAIAQNDIKKVLAYSTVSQLGYMFLACGVGAYVA
                     GMFHVLTHAFFKACLFLGSGAVIHALAGEQDVRKMGGLAPRLPVTYRTFLVATLALAG
                     IPPLAGFFSKDAILASVFAAHRPVLFAIGLVTAGLTAFYMFRLVSLTFYGEFRGTPDE
                     AHHVHEAPPTMTLPLVILAFLSAVGGFLGLPEVFGERANRVAAFLAPVLPPILLPGHE
                     AAGHHLPPSTEWLLIGISVAVAVAGLLLGWSWYAKEGGRPAARLAASFPGLYSLVAAK
                     FRVDELYDLLIVRPFTWLARVLWKVVDVLVIDGVLNAGAFLVELTGDALRFLQTGNVR
                     NYALTFLLGIVALVLFVIGGM"
     gene            complement(4137..4451)
                     /gene="nuoK"
                     /locus_tag="VF958_13365"
     CDS             complement(4137..4451)
                     /gene="nuoK"
                     /locus_tag="VF958_13365"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011522110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoK"
                     /protein_id="HEX9150146.1"
                     /translation="MMANGVPLGDVLLASGALFVIGLAGALLRRNAISIFLSIELMMN
                     AANLAVIGYAAALHSLEGQIVVFFVIAIAAAEAAVGLAIFVALFRHTETIDVNRINLL
                     KW"
     gene            complement(4448..4975)
                     /locus_tag="VF958_13370"
     CDS             complement(4448..4975)
                     /locus_tag="VF958_13370"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF012709.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit J"
                     /protein_id="HEX9150147.1"
                     /translation="MAGVTAARLVFDVAAGMAVIFASVMVVHRHPVKSVLALVVSFFA
                     LAVTYVLLAAPLIAAVQVIVYAGAILVLFLFVLMLLNVPEEPRQGSGRPIHVVLSSAA
                     IVVFAAALLSLLRAYGPAIAPLPAKAAWPAPGDLALLARLMFSEHLMSFEALSILLLA
                     ALVGAFALARRESAS"
     gene            complement(4963..6003)
                     /gene="nuoH"
                     /locus_tag="VF958_13375"
     CDS             complement(4963..6003)
                     /gene="nuoH"
                     /locus_tag="VF958_13375"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014559761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoH"
                     /protein_id="HEX9150148.1"
                     /translation="MTTTDWLVAAIRVLAPVLAIMLILPIPMWLERRGAGLIQDRPGP
                     NRVGPFGLLQPVADAVKFFMKEDLIPSNADRFLFVLAPGIALFSALTTFAVIPYGPSV
                     TVDGREIPLVGADVSIGILYLFALTSLSVYGIVLGGWSSNNKFSLIGGIRSSAQIISY
                     ELAMTTAAAGVILAAGSFRLTDVVAMQAGTWLGFIPRWNVFPQFFGFVVFYVATFAET
                     NRLPFDLPEAEAELVGGYHTEYSAFKFAMFFMAEYVNMIVASALTVTLFFGGWTLPGY
                     VPHGIVGVLVSALVFFAKTAFFVWVFIWVRWTLPRFRYDQLMRIGWKALLPIALLNLF
                     WIAFLVVKGWLA"
     gene            complement(6070..>6550)
                     /locus_tag="VF958_13380"
     CDS             complement(6070..>6550)
                     /locus_tag="VF958_13380"
                     /inference="COORDINATES: protein motif:HMM:NF012602.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HEX9150149.1"
                     /translation="PRAGGHGLEDVLAGRWTPQIAIVADAAFGAAAEDAEKVARLRQA
                     RFLAVAARTANALARAADVVLPAASLAQKEGSFTNAQGRVQRFERAFLPPPPARAHWE
                     FLLMLATALGWGDRNWTPADVRALIRSEIEGYGDVTEEELAGGGLMKKGLFVSTGAL"
BASE COUNT         1384 a         2057 c         2311 g          798 t
ORIGIN      
        1 cgacgagcag cagcagcagc gcgaacgcga cgagcgggtc cgcggcgagg tccggccgcg
       61 agagagccgc gacccgcacg ctccccgcct ttccgaagag gagcgcgatc ccgtagagcg
      121 tgaatgccga ggcgaacgct cccgtcagga agtacttcag gcccgcctcc gacgacgcct
      181 ggatggggcg gaagaacgcc gccaacacgt agaaagagag ggagagcgtc tcgagcccga
      241 tgaagacgac gaggaggtcg agtcctcgga tcatcagcag cacgccgaga tggccccaca
      301 ccaggagggc gtagaactcg cccttctctt cgccggtcct ttgaaggtac ggcttcgcaa
      361 ccaggatggc aacggccgca gtcgcggatg cgaagagacc cgcggccacc gtgaggtcgg
      421 aagtctcgta gcgtccggcg aacgagaggc ccgccggcgc ggcgacgaga aaccacgtcg
      481 aggcggcgat cgctgcgagc gagagggtcg cgagccagcg gcgagcggag ggagcgaagg
      541 catcgaggag gacggcgagg cagcccccca ccgccagggc gatctccggc gccgccgccg
      601 ccagatcgtt cggtccaaag gcgaggaagc tcggggtcac cgcggcacct cgagcccggg
      661 acgagcggaa ggcggaagcg ccgccgtctt ccgtgtggcc accgaccggc ggacctcctc
      721 gaccgacggt cgaaggcgat cgagaaagtc gttcgggcgg actccgatcc acaccatcag
      781 gaccacgaga gcggcagcgg cgacgagctc gctcggccgg atctcgggga gccgccggtt
      841 ctcctcccgg gtcaggggat tccagaaaac gcgctgcacg agagcgagca tgtaaacggc
      901 cgacaggatc actcccgtcg ccgccgcaat cgcccaccac cggtgattcg acgtccacgt
      961 ccccagcagg atcagaaact caccgacgaa cccgttcaac cccggaaggc cgatcgagga
     1021 gaaggtcgcc acgagaaaga gcgcagtcgt gaccggaacg acgccggcga tgccgccgta
     1081 gtcggcgatc tcgcgcgtgt gggtgcgctc gtacagcacg ccgacgagca ggaacagcgc
     1141 gcccgtcgag agcccgtgat tgatcatctg caggaccgag cccgtcatcg cggtgctcgt
     1201 gaaggcgccg atgccgatca cgacgagtcc gaggtgcgac accgaggagt acgccacgag
     1261 tctcttcatg tccgactgtg cgtacgcgac gcaggccccg tagatgactc cgatgacacc
     1321 gagaaccccg agccagggcg cataccgcag cgccgcgtcg gggaagaggg gaagcgcaaa
     1381 acgcaggtac ccgtagacgc cgagcttcag gaggatgccc gcgaggatga tggagccggc
     1441 cgtaggtgcc tccgtgtgcg cgtcgggcag ccacgtgtgg agagggaaga cgggcacctt
     1501 gatcgcgaat gcgagggcga acgcgaggaa gagcacggtt tggatcgcca tcgtcggata
     1561 ggtcagccgc gcgaggtccg cgagtaggaa ggaccagccg ccggttactg cgcggtgccg
     1621 gatggcgaga tacaggatcg cgaggaacat cagcaggctg ccggccatcg tgaagaggaa
     1681 gaacttcatg gctgcgtaga tccggcgccc gcccccccag atcccgatga tgaagtacat
     1741 cgggacgagc atgacctccc agaacacgta gaagagcgcg aggtcgagag caacgagcgt
     1801 ccccacgacg ccagtctcga gaacgaggaa cgccatcgag aaggcgcgca cgctcttcga
     1861 gacatgtgtg atcgagtaga ggagcgagac gggcgtcagc accgtcgtca gcaggaccag
     1921 cagtagcgat atcccgtcga ttccgacggt gtaggagatg ccgagcgccg gaacccactc
     1981 cgccttctcc acgaactgaa acgcacccga cgcgaggtcg aagcgcgacc agagggggag
     2041 agagagcgcg agctcgatga tcgacgccgc gagggcgaag gtcttcgccc cgcgcgcccg
     2101 gctctccgga aagaggagca gcaatgccgc cgccacggtc ggcaggaaaa ccaggacggt
     2161 caacaggtgt tggtcgaaca gggcgggcaa cctacatgcc tccgatgacg aagaggacga
     2221 gcgcgacgat gccgaggagg aacgtgagcg cgtagtttcg gacgtttccc gtctggagga
     2281 accggagcgc gtcgcccgtg agctcgacga gaaacgcgcc ggcgttcaag actccgtcaa
     2341 tgacgaggac gtcgacaacc ttccagagga cgcgcgcgag ccacgtgaag ggccgcacga
     2401 tcaagaggtc gtacagctcg tcgacccgga acttcgcggc gaccagcgaa tagaggcccg
     2461 ggaacgaggc ggcgaggcgc gcggcggggc gtcccccctc cttcgcgtac caactccaac
     2521 cgagaagaag tccagccacg gcgacggcga ccgagatgcc gatcaggagc cattccgtcg
     2581 atgggggaag gtggtggccc gccgcctcgt gccccggaag caatatcggc gggaggacgg
     2641 gcgcgaggaa cgcggcgact cggttcgcgc gctcgccgaa gacttccggg agcccgagga
     2701 agccgccgac cgccgaaagg aacgcgagga tgacgagggg gagcgtcatg gtcggcggcg
     2761 cctcgtgaac gtgatgggcc tcatcgggcg tcccccggaa ttcgccgtag aaagtgagcg
     2821 agacgagtcg gaacatgtag aacgccgtca ggcccgcggt cacgagtccg atcgcgaaga
     2881 ggaccgggcg gtgggcggcg aacacgctcg cgaggatggc gtccttcgag aagaagccgg
     2941 cgagcggcgg gatcccggcg agagcgagcg tggcaacgag gaacgtccga taagtgacgg
     3001 gaaggcgcgg cgcgaggccg cccatcttcc ggacgtcctg ctcaccggcg agcgcgtgaa
     3061 tgacggcgcc ggacccgagg aagaggcacg ccttgaagaa cgcgtgcgtc aggacgtgga
     3121 acattccggc gacgtacgcg ccgaccccgc acgcgaggaa catgtaaccg agctgggaaa
     3181 cggtggagta cgcgaggacc ttcttgatgt cgttctgcgc gatggcgatc acggcagcga
     3241 agatggcggt cacgccgccg atgatcgcga cgaccgacat cgcctccggg gcgagccgga
     3301 agagaacgtt gcagcgcgtc accatgtaga cgccggcggt caccattgtc gccgcgtgga
     3361 tgagggcgga cacgggcgtg ggtccggcca tcgcgtccgg gagccagacg tggaggggaa
     3421 cctgggcgct cttgcccatc gcgccgagga agaggagcag gcaaacgacg gtgagacccg
     3481 gggcgaatct tcccggatct gccgctgccc gcggaaacac accgtcgaag tcaagcgtcc
     3541 cgaacagcga gaacacgccg aacatcccga gcaggaaacc gaagtcgccg atccggttga
     3601 cgacgaacgc cttcttcccg gcggcggcag cgaagtcttt ttcgtagtag tagccgatga
     3661 ggaggtagga gcagaggccc accccttccc atccgacgaa catgagcagg aaattgttcg
     3721 cgagcacgag ggtcagcatc gagaacagga agaggttcat gtaggcgaag aaccgcgcgt
     3781 acccggcgtc ggtttcgtcg tgtcgcatgt agccgatcga gtagacgtgg atgaggaagc
     3841 cgacgaaagt cacgaacgag accatcagcc cggacagggg atcgagacgc agggagatgt
     3901 ccacggagaa gttgcccgcg gcgatccagg gggcgatccg ctcgaccccg gcggtgccgc
     3961 ctcccgcgaa gtagggaatc aggcgccacc acgcggcgag tgtcgcgagg ccgaccgatc
     4021 cgactccggc cgccgtcacg aagctctttc caaggcggcg accaaggagg aggcagaaca
     4081 ggaatcccgc gagcggcagc gccgggatga cccagagggc gagcgtcacg ccggcatcac
     4141 cacttgagga ggttgatccg gttcacgtcg atcgtctccg tgtgccggaa gagcgcgacg
     4201 aagatcgcga ggccgacggc ggcctctgcc gccgctatcg cgatcacgaa gaagacgacg
     4261 atctgcccct cgaggctgtg gagggctgcg gcgtaaccga tgaccgcaag gttcgcggcg
     4321 ttcatcatga gctcgatcga aagaaagatc gagatcgcgt tgcgtcgcag cagcgcgccc
     4381 gcgaggccga tcacgaagag cgcgccggag gcgaggagca cgtcgcccag cggaactccg
     4441 ttggccatca tgacgcgctc tcccggcgcg cgagggcgaa tgctcccacc agcgccgcga
     4501 gcaggaggat cgacagcgcc tcgaacgaca tcaggtgctc cgagaacatg agacgcgcca
     4561 ggagcgcgag gtcgccgggc gccggccagg ccgctttcgc aggaagggga gcgatggccg
     4621 gcccgtaggc gcggaggaga gaaagaagcg cagccgcgaa aaccacgatc gcggcggagg
     4681 agaggaccac gtggatcggc cgtcccgacc cctgccgcgg ctcctcgggc acgttcagga
     4741 gcatcaggac gaagaggaaa aggacgagga tcgctccggc atagacgatc acctgcacgg
     4801 ccgcgatcag cggagccgcc agcaggacgt aggtcacggc cagtgcaaag aacgacacga
     4861 cgagcgcgag cacgctcttg actggatggc ggtggacgac catcacgctc gcgaagatga
     4921 cggccatccc ggcagcgacg tcgaaaacga gccgcgccgc cgtcacgcca gccatccctt
     4981 gaccaccagg aacgcgatcc agaagaggtt cagcagggcg atcggaagga gcgccttcca
     5041 gccgatccgc atgagctggt cgtagcggaa gcggggcagc gtccacctca cccagatgaa
     5101 gacccagacg aagaacgccg tcttcgcgaa aaagacgaga gccgaaacga ggacgccgac
     5161 gatgccgtgc ggtacgtagc ccggcagggt ccagccgccg aagaagaggg tgaccgtgag
     5221 cgcggaggcg acgatcatgt tgacgtactc ggccatgaag aacatggcga acttgaacgc
     5281 cgaatactcg gtgtggtacc cgcccacgag ctcggcctcc gcttcgggga ggtcgaaggg
     5341 cagccggttc gtctccgcga acgtggcgac gtagaagacg acgaacccga agaactgagg
     5401 aaagacgttc caccggggga tgaaaccgag ccacgtcccg gcctgcatcg cgacgacgtc
     5461 ggtcaggcgg aaggatccgg cagcgaggat caccccggcg gccgcggtcg tcatcgcgag
     5521 ctcgtaggag atgatctgcg cgctcgaccg gattccgccg atgagcgaga acttgttgtt
     5581 cgaggaccag cccccgagca cgatcccgta cacggagagt gacgtcagag cgaagagata
     5641 caggatgccg atcgagacgt ccgcgccgac gagcggaatc tcgcgcccgt cgacggtcac
     5701 gctcggtccg tatgggatca cggcgaaggt cgtcagcgcg gagaagagcg cgatccccgg
     5761 cgcgagcacg aagaggaagc gatcggcgtt cgacgggatc aggtcctcct tcatgaagaa
     5821 cttgaccgca tccgcgaccg gctgcagcag cccgaacggt ccgacgcggt tgggacccgg
     5881 ccggtcctga atcaggcccg cgccgcgcct ctcgagccac atcggtatcg gcaggatgag
     5941 catgatcgcc aggacgggcg cgaggacccg gatcgcggca acgagccaat cagtagtagt
     6001 caacgggtcc ctcggtgcgt cgctggatca cgccggtggg tgcaggccat tcgcaagcga
     6061 gcgcgacggt caaagcgctc ccgtcgacac gaagaggccc tttttcatga ggcctccgcc
     6121 ggcgagctcc tcttcggtca cgtctccgta gccctcgatt tcgctgcgga tgagcgctcg
     6181 gacgtcggcg ggagtccagt ttcggtcgcc ccagccgagc gccgtcgcga gcatgaggag
     6241 gaactcccag tgcgcgcgag cgggcggagg aggaagaaat gcgcgctcaa acctctgaac
     6301 gcggccctgg gcgttcgtga acgagccctc cttctgtgcg agcgacgcgg cgggcagcac
     6361 gacgtccgcc gcccgcgcca acgcgttcgc cgtccgggcg gcgacggcga ggaaccgcgc
     6421 ctgccggagc cgggcgacct tttccgcgtc ctcagcggcg gctccgaacg cggcgtcggc
     6481 gacgatcgcg atctgtggag tccagcggcc cgcgagcacg tcttcgagcc catgcccgcc
     6541 ggcgcgcggc
//
LOCUS       DASWAV010000642         3059 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U2665
            k141_677192, whole genome shotgun sequence.
ACCESSION   DASWAV010000642 DASWAV010000000
VERSION     DASWAV010000642.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960316
            Sequence Read Archive: SRR10260007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 3059)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3059)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:47:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,929
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3059
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_677192"
                     /isolate="SMAG_U2665"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.36444 N 132.31889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..729)
                     /locus_tag="VF958_13385"
     CDS             complement(<1..729)
                     /locus_tag="VF958_13385"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF019334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEX9150150.1"
                     /translation="MIGATLLHYRIVEALGRGGMGEVYAAEDTKLGRRVALKLLPREM
                     AADTERLRRFQREAQALALLNHPNLVTIYAVEEAAETHFITMELVEGKTLGELTPPRG
                     WPIREFLRLAIPLADAVGTAHLRGIIHRDLKPANVMVGSDGRVKVLDFGLAKLKQEDA
                     GSADVSALTTTEQLTAKHSVFGTAAYMSPEQAEGRSLDHRTDIFSLGVVLYELATGRR
                     PFHGDTAVSVISSILKDSPPPLAQV"
     gene            complement(1172..1247)
                     /locus_tag="VF958_13390"
     tRNA            complement(1172..1247)
                     /locus_tag="VF958_13390"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1212..1214),aa:Ala,seq:ggc)
     gene            1324..1421
                     /locus_tag="VF958_13395"
     tRNA            1324..1421
                     /locus_tag="VF958_13395"
                     /product="tRNA-Sec"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1358..1360,aa:Sec,seq:tca)
     gene            1489..1986
                     /locus_tag="VF958_13400"
     CDS             1489..1986
                     /locus_tag="VF958_13400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9150151.1"
                     /translation="MSDKILGFLPPLIVGISFIYMIGAVVTGVLESRHRALAIRARTD
                     LLNRILDKFGSSRDFIELSDSPGGKRLLEALGSEPNGVGSKILTAVQRGVVLTVVGIG
                     VLILSLTITENAVEEFIRIGGAVLLALGIGYLGSSAISYRMAKSIGLLAPHGDPSASD
                     AGRLA"
     gene            2009..2587
                     /locus_tag="VF958_13405"
     CDS             2009..2587
                     /locus_tag="VF958_13405"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEX9150152.1"
                     /translation="MIVDAALGGSPFLATADAGATPVTQAEFEDFYAETSRPFWGYLR
                     RICGDRALADDLAQEAYLRFCRARVPESRSDWKAYLYRIGTNLAADWWRHTGRRESSG
                     FENASAPSPSEGRDEEADAIRRHELDRAFSSLKPRERALLWLAYVEGLDHREIAKALT
                     LSRASVRVLLFRARRKLAERLERKGIGPEVLR"
     gene            2584..2961
                     /locus_tag="VF958_13410"
     CDS             2584..2961
                     /locus_tag="VF958_13410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9150153.1"
                     /translation="MKNSEPIEEKRLAAALSALGVPDGEQPELPDAAILWRKARLLEA
                     LETRRLAVRPVRVAHSIALALASGALLALGGSKGVSLLGWIEKSGAGLTLPLTLSLFV
                     LGAGLFLATGAAAGRNLSRRTGE"
BASE COUNT          481 a         1006 c         1058 g          514 t
ORIGIN      
        1 cacctgcgcg agcggcggcg gcgaatcctt gaggatcgag gagatgacgg agaccgccgt
       61 gtcgccgtgg aacggccgcc ggcccgtcgc gagctcgtag aggacgacgc ccagcgagaa
      121 gatatcggtg cggtgatcga gcgaacgccc ctcggcctgc tcgggggaca tgtaggcggc
      181 ggtcccgaag accgaatgct tcgccgtgag ctgctcggtc gtcgtcaggg cgctgacgtc
      241 cgccgagccc gcgtcctcct gcttcagctt cgcgaggccg aagtcgagga ccttcacccg
      301 gccatcgctc ccgaccatga cgttcgcggg cttcaaatcg cggtggatga tgccgcgcag
      361 gtgagcggtg ccgacggcgt cggccagggg gatggccagc ctcaggaatt cccgaatcgg
      421 ccaccctcga ggcggcgtga gttctcccaa agtcttcccc tcgacgagct ccatggtgat
      481 gaagtgagtc tccgcggcct cttcgacggc gtagatcgtc acgagattgg gatggttcag
      541 gagggcgagg gcctgggctt cccgctggaa ccggcggagc ctttccgtgt cggccgccat
      601 ttctcgcggc aggagcttga gggcgacccg gcgacccagc ttcgtgtcct cggccgcgta
      661 gacctcgccc attccaccgc ggccgagagc ctccacgatg cggtaatgaa ggagcgtcgc
      721 cccaatcatg gcgtcgcctc gccagcaact tccgtcgggc cgtggaaagg agagattcgt
      781 ccgcccgacg gtcgagcgat tggaattcta tcgcccgctc ggctcagccc ctggagtcgg
      841 cgtacattcg cggcgacgtc ggggttgaaa ccggagcatt tgatccaggg ttcgcgggag
      901 cgagggaggg atcaggctcg gtcgaggcgg gtttgccgag ccgagcgtcg gtggcggagc
      961 tagatcccga gcgaacgacc gaacaatggt ggagctgaac gggatcgaac cgtcgcgtat
     1021 ctggggttcg cagcgcgaga agtctcccgg agggaggagg ctcgcgctgc gggagcggga
     1081 gtgagcgagg gagcaggcga gcggtcgagc cgggtgtggc gagccgctcg ccggtgcgtc
     1141 agcagatccc gagcgagcga gagcgacgtg ttggtggagc tgaacgggat cgaaccgtcg
     1201 acctcctgaa tgccattcag gcgctctccc aactgagcta cagccccacg gtcggagggc
     1261 gcgcaggata gcacggcgtt cgcgccagct tcaacgaatc cacttccggc ataatccgcg
     1321 cgcggaagcg cccggggtct ggtggccctc gcggacttca aatccgttgg cgtcccggct
     1381 tcgccgggat gggtgggttc gattcccatg cgcttccgcc aatttcgtcg tcggtcgcgt
     1441 taaccccgat ggcctcccgt ccgtttcaat catcgagaga ggcaaaccat gagcgacaag
     1501 atcctcggtt tcctgccgcc tttgatcgtc ggaatttcct tcatctacat gatcggcgcg
     1561 gtcgtcacgg gcgtgctgga gtcgcgacac cgcgcgctcg cgatccgcgc ccggacggat
     1621 ctcctgaacc ggatcctgga caagttcggg tcgagccggg atttcatcga gctctccgac
     1681 tcgcccggcg gcaagcgact gctcgaggcg ctcgggagcg agccgaacgg cgtcggatcg
     1741 aagatcctca cggccgttca gcgcggagtg gtgctgaccg tggtcgggat cggcgtcctc
     1801 atcctctcgc tcacgattac cgaaaatgcc gtcgaggagt tcattcgaat cggcggcgcc
     1861 gtccttctcg ccctcggaat cggctatctc ggatcgagcg cgatctccta ccgcatggcg
     1921 aagtcgatcg ggctgttggc gccgcacgga gatccgtcgg cgtccgacgc cgggcgtctg
     1981 gcgtgaacga cccttccccg gaacggcgat gatcgtcgat gccgcgctcg gaggctctcc
     2041 gtttctcgcc acggccgacg cgggagccac tcccgtgacg caggccgagt tcgaggactt
     2101 ctacgccgag acctcgcggc cgttctgggg atatctccgt cggatctgtg gcgaccgggc
     2161 tctcgccgac gacctcgccc aggaggcgta cctgcgcttc tgccgggcgc gggtccccga
     2221 gtcccggtcc gactggaagg cctacctcta ccggattggc acgaacctcg ccgctgactg
     2281 gtggcgacac acgggacgcc gcgagtcgag cgggttcgag aacgcgtcgg cgccctctcc
     2341 ttcggaaggc cgcgacgagg aagccgacgc gatccgccgc cacgagctcg accgcgcctt
     2401 ctcctctctc aagccgcggg agcgcgccct tctctggctc gcttacgtgg agggcctcga
     2461 tcaccgggag atcgcgaagg cgctcacgct ctcgcgagcg agcgtccggg tgctgctctt
     2521 ccgggcccga cggaaactcg ccgaacggct cgagcggaaa ggaatcgggc ccgaggtgct
     2581 gcgatgaaga actccgaacc gatcgaagaa aagcggctcg cggcggcgct ctcggctctc
     2641 ggcgttccgg acggggaaca gccggagctc ccagacgcgg cgatcctctg gcggaaggcg
     2701 cggctcctgg aggcgctcga aacgaggcgc ctcgccgtgc ggccggtccg cgtggcgcac
     2761 tcgatcgccc tcgcgctcgc ctcgggcgcg ctgctcgccc tggggggctc gaaaggcgtg
     2821 tcgctactcg gctggatcga gaagtccgga gcgggcctga cgctcccgct cacgctctcg
     2881 ctcttcgtgc tcggtgccgg actgtttctc gcgaccggcg ccgcggccgg acgcaacctc
     2941 tcgcgccgca cgggcgaata gacgctgcgg ctatgatctc gcgtcggaaa gacgcgaatg
     3001 aagaacggga cgctcgccgc tctgatcctg ggatccgtcc tcgccgtggg catgatcgg
//
LOCUS       DASWAV010000643         1747 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U2665
            k141_238490, whole genome shotgun sequence.
ACCESSION   DASWAV010000643 DASWAV010000000
VERSION     DASWAV010000643.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960316
            Sequence Read Archive: SRR10260007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 1747)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1747)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:47:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,929
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1747
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_238490"
                     /isolate="SMAG_U2665"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.36444 N 132.31889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            299..691
                     /locus_tag="VF958_13415"
     CDS             299..691
                     /locus_tag="VF958_13415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9150154.1"
                     /translation="MDRETAEEIKQHFDVVAEGLRSEMHEIAGGLRTEIRLGDEGLRS
                     EMHEIAGGLRSEIRLVDEKVDRSSVDLKRHFEVVGESLRSDIRTVAEGFVSTNERLDR
                     IESAMSEKFGELEVMIRLSFGELAQRIH"
     gene            770..1135
                     /locus_tag="VF958_13420"
     CDS             770..1135
                     /locus_tag="VF958_13420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9150155.1"
                     /translation="MDHETTEEIKRYLDVTAANLRGELKADIREVREDLLVEIQEVRE
                     DLRVKIHEVKRHADVVAESLRSEIRLVAEGVAGLDEKFTQEFVNVRTEIREQIGDVKA
                     LIRGGYADLDRRVTKKRPT"
     gene            1247..1483
                     /locus_tag="VF958_13425"
     CDS             1247..1483
                     /locus_tag="VF958_13425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015744035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9150156.1"
                     /translation="MKTAVSVPDPIFERAEKLARRMRKSRSQLYSDALREYTARHDAA
                     AITTALDALYEGEDSGPEAFVRESARRTLRSTDW"
     gene            1484..>1747
                     /locus_tag="VF958_13430"
     CDS             1484..>1747
                     /locus_tag="VF958_13430"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009546320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004521 - RNA endonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HEX9150157.1"
                     /translation="MVVARGDVFWADLPEPTGSGPGLRRPVLIVQGNAFNRSRIATVV
                     VVPLTSNLRLASAPGNVALRARATGLPKDSVANVSQIVTIDRGL"
BASE COUNT          356 a          514 c          584 g          293 t
ORIGIN      
        1 gagctgtttc agcgcgccgt ggaggaggtg accgcggccg cggagcggct gattcgggga
       61 tgggtcacca gcgcgccggc gaaggatcgc gacgtcgaga ttcggaagat gatcgagaaa
      121 tcgcgggtgc gattcggcaa ggagcccagg cgcaccccga tcgatccgat tcggtaggtc
      181 tcgactccgg ttaactctct cgcgccagga ccgggcggga aatcggggac agaccacggt
      241 ttcccgccgc gacaatactg atcagcgccg gtgctaggct cgaccgacac gaggacggat
      301 ggatcgcgag acagccgaag aaatcaaaca gcatttcgat gtcgtggcgg aagggctgcg
      361 gtcggaaatg cacgagatcg caggaggtct gcgaaccgag atccgcctcg gggacgaagg
      421 gctgcggtcg gaaatgcacg agatcgcagg tggcctgcga agcgagatcc gcctcgtgga
      481 cgagaaggtc gatcggagct ccgtcgatct gaaacgccat ttcgaggtcg tgggcgagag
      541 tctccgatcg gacatccgca cggtcgcgga agggttcgtc tcgacgaatg agcgactcga
      601 tcggatcgaa tcagcgatgt ccgaaaagtt cggcgagctc gaggtgatga tccgcctctc
      661 gttcggcgag ctcgcgcaac gaattcatta gggcgggaaa tcggggatag agcacgtttc
      721 ccggcgtcgc gacaatactg gaacaggtgc taggctcgat ccgagatcca tggaccacga
      781 aacaaccgaa gagatcaagc gctatctcga tgtcaccgct gcgaatcttc gcggggagct
      841 gaaggcagac attcgagagg tccgggagga cctcctcgtt gagatccagg aggttcggga
      901 ggacctccgc gttaagatcc acgaagtcaa gcggcacgcc gacgtcgttg cggaaagtct
      961 gcgctccgag atacggctcg tcgccgaagg ggtcgccggt ctggacgaaa agttcacgca
     1021 ggagttcgtc aacgttcgca cggagatccg cgagcagatc ggcgacgtga aggcactcat
     1081 ccgtggcggt tacgccgacc tcgatcgccg ggtcacgaag aaaagaccga cgtaaagacc
     1141 gcgccggtca ctccgccttg agcccgagtg ggatccggtt agagggtgtc ctcatctgcc
     1201 gcaatatccg cgcccacgtc ccgattccct cggttatact aatggtatga aaacggcggt
     1261 ttccgtcccc gaccccatct tcgagcgggc cgagaagctt gcccgtcgga tgcgaaagtc
     1321 cagaagccag ctgtacagcg acgcgttgcg ggagtacacc gcgcgtcacg acgcggcggc
     1381 gatcacgacg gccctcgacg cgctctacga aggagaagat tccggaccgg aggcgttcgt
     1441 acgcgagagc gctcgtcgga cccttcgctc gacggactgg tgagtggtcg tcgcacgggg
     1501 agacgtcttc tgggccgatc ttcccgagcc gacgggctcg ggccccggcc ttcggcggcc
     1561 cgtcttgatc gtgcagggca atgccttcaa tcggagccgc atcgcgacgg tcgtcgtcgt
     1621 ccctttgacc agtaacctcc gtctcgcgag tgcgcccggc aacgtggctt tgcgggcccg
     1681 ggccaccgga ctgccgaaag attccgtggc gaacgtctcg caaatcgtca cgatcgaccg
     1741 cggactt
//
LOCUS       DASWAV010000644         1908 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U2665
            k141_639649, whole genome shotgun sequence.
ACCESSION   DASWAV010000644 DASWAV010000000
VERSION     DASWAV010000644.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960316
            Sequence Read Archive: SRR10260007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 1908)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1908)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:47:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,929
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1908
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_639649"
                     /isolate="SMAG_U2665"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.36444 N 132.31889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(70..831)
                     /gene="mazG"
                     /locus_tag="VF958_13435"
     CDS             complement(70..831)
                     /gene="mazG"
                     /locus_tag="VF958_13435"
                     /EC_number="3.6.1.9"
                     /inference="COORDINATES: protein motif:HMM:NF007113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphohydrolase"
                     /protein_id="HEX9150158.1"
                     /translation="MPEELSRLVALVEQLRRECPWDREQTPETLRTYLLEECYEVLDA
                     LDSGDPNALADELGDLLFQILFLSRIAQERGWFSIQDVAAKISAKMVERHPHVFGDTR
                     ASDADEVRTGWERRKQRASGASDPLGSLPPSLPALAASYRMTSRAADLGFDWEKDADV
                     AAKIEEELGEWREAERAGDRDAEEKEIGDLLFSIVNLARRRKVDPESALRRAQARFRS
                     RFAKVAERARESGRTVGDVSPAELDEYWERAKAEE"
     gene            891..1358
                     /locus_tag="VF958_13440"
     CDS             891..1358
                     /locus_tag="VF958_13440"
                     /inference="COORDINATES: protein motif:HMM:NF020473.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1844 domain-containing protein"
                     /protein_id="HEX9150159.1"
                     /translation="MSEGEDRPIRVVDRRMFTPEGELRPGYEPEESAEPTAPAAAPSE
                     PPPPAEPPRASPQAAAPAEAERPPDSGESRSEFARIVSSLATTAYSALGLLSDPAAGA
                     RHRDLAVARQMIDWLAVLEQKTRGNLSFEESDLLSRVLYELRLAYVEVGRAPK"
     gene            1432..>1908
                     /locus_tag="VF958_13445"
     CDS             1432..>1908
                     /locus_tag="VF958_13445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9150160.1"
                     /translation="MSPLPPPVTRALYRSQWFELLDHFQESDERAARTTLDVMIKAAR
                     STGVRRLADYARAAVHVARSAEQSGRPGGARLAYEAAVALDDTSFDAAAARLSFFVRS
                     GRFGEAFRTLPSAVSALFSSSEARLSFTSSLALVIAAALAAASLATALALFLRHSRR"
BASE COUNT          259 a          680 c          643 g          326 t
ORIGIN      
        1 gttccggtct cgcggctcgc tcactccggc aagacccggg tcaaggtcgc ggccctcgaa
       61 ccttgaggtc tactcctccg ccttcgcccg ctcccagtac tcgtcgagct ccgccgggct
      121 gacgtctccc acggtccggc cggattcgcg cgctctctcg gcgactttcg cgaaacggct
      181 tcggaatcgc gcctgagcgc gccgcagcgc gctctccgga tccaccttcc gtcggcgcgc
      241 gagattgacg atcgagaaga gaaggtcgcc gatctctttt tcctccgcgt cgcggtcccc
      301 ggctctttcc gcctcgcgcc actcgccgag ctcctcttcg atcttggcgg cgacgtcggc
      361 gtccttttcc cagtcgaagc cgagatcggc agcccgcgac gtcatccggt aggaggcggc
      421 gagggccggg agagatgggg ggaggctgcc gagagggtcc gaggctcccg aggcgcgctg
      481 cttgcgccgt tcccagcccg ttcgaacctc gtcggcgtcg gacgcccgcg tgtccccgaa
      541 gacgtggggg tgccgttcca ccatcttcgc ggagatcttc gcggcaacgt cctggatcga
      601 gaaccacccc cgctcctgag ctatccgcga gagaaacagg atctgaaaca ggaggtcgcc
      661 gagctcgtcg gcgagcgcat tcgggtcgcc ggagtcgagc gcgtccagaa cttcgtagca
      721 ctcctccagc aggtaagtgc gcagggtttc gggcgtttgt tcgcgatccc aggggcattc
      781 gcggcggagt tgctcgacga gagcgacgag gcgggaaagc tcttccggca ctcgcgaatg
      841 atagcgatcg gccgacggca gcgcggggag agctaaaatc cggtgcaaat atgagcgaag
      901 gagaggacag gccgatccgg gtggtggacc ggaggatgtt cactccggag ggggagctgc
      961 gccccgggta cgagcccgaa gagagcgccg aaccgacggc acccgccgcg gcgccctccg
     1021 agccaccgcc gccggcggag cctcctcggg cttcccctca agcggctgcc ccggcggagg
     1081 ccgagcggcc cccggattcc ggcgaatccc gcagcgagtt cgcgcgcatc gtgagttcgc
     1141 tcgccacgac tgcatactcg gctctcggct tgctctccga cccggcggcg ggcgcccggc
     1201 accgggacct cgcggtcgcg cggcagatga tcgactggct cgcggtcctg gagcagaaga
     1261 cgcggggaaa cctctccttc gaggagtccg atctgctctc gcgggtcctg tacgagcttc
     1321 gtctcgcgta cgtggaggtt ggccgcgctc cgaagtagcc ggcaactacc ggtcctcgtt
     1381 tttctcttct gcgccggagt cctctctggg gagccggcgt cggaggcgcg cgtgtctccg
     1441 cttccgccgc cggtgacgcg cgcgctctac cgatcgcagt ggttcgagct gctggaccac
     1501 ttccaggaga gcgacgagcg tgccgcccga acgaccctcg acgtgatgat caaggccgcg
     1561 cgttcgaccg gcgttcgccg cctcgccgac tatgcgcgcg ccgccgtcca cgtggcgcgg
     1621 agcgcggagc agtccggccg ccccggcgga gcccggctgg cttacgaagc cgccgtcgct
     1681 ctcgacgaca cgagcttcga cgccgcggcc gcgcgtctct ccttcttcgt tcgcagtggc
     1741 cgcttcggcg aggcgttccg gacgcttccg tcggcggtga gcgcactttt ttcgagctcg
     1801 gaggcgcggc tgtcgttcac gtcctccctc gcactcgtca tcgcggcggc cctggcggcc
     1861 gcttccctgg cgaccgcgct cgcgttgttc ctccggcact cccgccga
//
LOCUS       DASWAV010000645        12152 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U2665
            k141_557448, whole genome shotgun sequence.
ACCESSION   DASWAV010000645 DASWAV010000000
VERSION     DASWAV010000645.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960316
            Sequence Read Archive: SRR10260007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 12152)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12152)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:47:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,929
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12152
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_557448"
                     /isolate="SMAG_U2665"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.36444 N 132.31889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1596)
                     /locus_tag="VF958_13450"
     CDS             complement(<1..1596)
                     /locus_tag="VF958_13450"
                     /inference="COORDINATES: protein motif:HMM:NF013106.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HEX9150161.1"
                     /translation="MPQNAGVITGRKAVIAVVPLLAAALLGVVAGSAATNFVRVPRVS
                     ELETYRPDIITEIRGSDGSTIARYAIERRILVSRGQIPAVLRNAIIATEDKNFFRHGG
                     VDVQRTVSALVANLKQKSFAQGGSTLTQQLARAIFLSPRKTISRKINEAFVAFEIERR
                     YSKEQILTMYANEIYFGHGNYGVEAASRYYFGKGIKDLTLAEAALLAGIVQRPEDQSP
                     FRNPTQARRRRATALARMKAEGYITDADERAANGEPLPTTPSLEETIVGPYYCEEIRQ
                     YLQRTYGEQELYRRGLRVESTLDPQIQQWSDEALRWGLRRLARRHGFQKPRNLLDEGY
                     KDLESFVDPSWESASRGSGGAMHGIVLSVSRSGGEIRVGTRVLPILPAGYAWTSATDP
                     SKILKRGDLVAISIEQRKDGSELLSLENDPKEEGAVLVIENSTGAVRAMTGGYDWTRS
                     KFNRATQALRQAGSTFKPFVYLAALEAGYTPSDTVFDGPISIVYDPRQPPYRPTNYDG
                     KFRGIVTFRRALEYSYNVSAVRVA"
     gene            1628..2098
                     /locus_tag="VF958_13455"
     CDS             1628..2098
                     /locus_tag="VF958_13455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9150162.1"
                     /translation="MSTPPPIEPAPAGLPANRGCWKGALYGCGAVAVCIVAVLVGLGI
                     YVQKRPGAITDMLMERIRDRYASDVTAQDKADLEAAYADFRTALEAKRVRRDDLDRVR
                     FTVKLGREVRHDEVVELTRVFREAAENGGRDSGQGTREAMPAATPPPVAATPVP"
     gene            2206..4179
                     /gene="uvrB"
                     /locus_tag="VF958_13460"
     CDS             2206..4179
                     /gene="uvrB"
                     /locus_tag="VF958_13460"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020719225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HEX9150163.1"
                     /translation="MKTDFTPQGDQLQAIDHLARGIDEGRRDQVLLGVTGSGKTFTMA
                     KVIEAVNRPTLVLSHNKTLAAQLYQEFRGFFPENAVEYFVSYYDYYQPEAYIPQTDTY
                     IEKETSRNEEIDKLRLSASKALFERRDVIVVASVSCIYGLGSPESYYDMLAYVEVGDR
                     SGMRALLAQLVEMQYERTNLDLARGTFRVRGDVLEIHPAYEDVGVRIEYFGDEVEKVT
                     RTDPLRGTALQKVQRIALYPRTFYATPRETLERAIGTIRLELDARLAELSAAGRLLEA
                     QRLHQRTMFDLEMIKELGYCNGIENYSRHLSGRQPGEPPPTLIDYFPRDFLLVIDESH
                     VTLPQVRGMYHGDRSRKEVLVDYGFRLPSALDNRPLTYEEFEARLNQIVYVSATPGPE
                     ELGKTGGEVVEQVIRPTGLLDPVIEVRPVKGQVDDLLAVVRDAAAKSQRVLVTTLTKR
                     MAEELTNYFSEVGVRCRYLHSDIETLDRVAILMDFRRGVFDCLIGINLLREGLDLPEV
                     AVVAILDADKEGFLRSQTSLIQTAGRAARNLEGRVIFYADAVTDSMRRAIEETGRRRK
                     IQEAYNREHGIEPASIVKQVNSPLVRMSSLDYYDPTPALSRPDLPDAPSLGRRIVELE
                     KAMRAAAKELDFERAAEIRDELRGLRELQIFRS"
     gene            complement(4231..4590)
                     /locus_tag="VF958_13465"
     CDS             complement(4231..4590)
                     /locus_tag="VF958_13465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9150164.1"
                     /translation="MTMRTRGLFLAAGFAALLGFAPTARAQWMRLQRCSGALPCAIPF
                     GIRYAPDPLIASQYGRMSPDGFSGRISFDPQLTVEIDEPRLSLEPADFAARAAREFVL
                     AHPAPPASTPAPAKNPD"
     gene            complement(4684..4890)
                     /locus_tag="VF958_13470"
     CDS             complement(4684..4890)
                     /locus_tag="VF958_13470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9150165.1"
                     /translation="MNECPCCYTTVGPQDSVCPKCGREIERWQTGFYTRQPLGTKKKT
                     AVRVVLVIAVLVVVGGFARACHLL"
     gene            4964..6826
                     /gene="uvrC"
                     /locus_tag="VF958_13475"
     CDS             4964..6826
                     /gene="uvrC"
                     /locus_tag="VF958_13475"
                     /inference="COORDINATES: protein motif:HMM:NF001824.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HEX9150166.1"
                     /translation="MSDSRRPPENVPDAPGVYLFKDRRGKTLYVGKARSLKKRVASYF
                     VRGPEVTKTEALLDSFAEVETIVTNTELEALLLENSLIKKHRPRYNICLRDDKTYPYV
                     KITTGEDWPRALVTRRVAEDGHSYFGPFWGGLARRIMRMITRHFQIRTCTIEIDGTLP
                     RPCLYYDLHACLGPCVAGLTSKAAYDEAVRDVVLFLQGRNRELLASLEKKMLQASSEE
                     NFEMASAYRDALRTVEDVSEHQIVHSLKGESVDVFGFFESGGDVAVCALVVRGGVIQD
                     RREFFFEKSEEVDPGVFFDAFLPQFYDANPFLPAEVHLPVAIAEPDLLETFLSSRRGA
                     RVSVRVPRRGPARERVELAQDNARERHKVRFRRMGGEGALGVERLARILDLPVPPRRI
                     EAFDISHLQGTDSIASLVVFEDGKPKKSDYRLFGIASQELLAPDDFRSMAEAVERRYR
                     KVRDEGIEVPDLVLVDGGRGQLQAALTALDRLGVELPVVGLAKREEEIWVPGRPDPIR
                     LPRKDPALQLVQRVRDEAHRFAITRHRGRRSKRMRETSLMEIPGIGPTRARTLLRRFG
                     SLSGLAAADPKDVETAVGPASARAVREYLQAARRQDEPAALPGGLGEGGRVRSA"
     gene            6823..8526
                     /locus_tag="VF958_13480"
     CDS             6823..8526
                     /locus_tag="VF958_13480"
                     /inference="COORDINATES: protein motif:HMM:NF025690.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4388 domain-containing protein"
                     /protein_id="HEX9150167.1"
                     /translation="MRTAFEREGDIRSVDLTGALAALWQERATGVLAFASGEERVRFE
                     ILEGELVGAFSSDPSFDTAEVLIRGGKLDGGALEGRQFPPGRDRARAARELGVLTERD
                     WRWGEKIRAVEILSDLIGWLDGRYSFDPGERPAAGDFQLGIERLLLELFLRSRDREFV
                     HRSLGAVDAPLQRADDFEDRFRSLGLTTDAELVVAVIDGRATAAEISRRVGPDPFSVE
                     KLLAALTTLGLVHPEYAAPPKAKRSGPAKVPPAALPAPVAEPEPPRPEPETPAQGATS
                     VPEAVAPAAPAPPVESPSNEVEPLAEAFEEPVLGPEPVELPLLPVPEPTETEPVLTSW
                     EPIPPEPLDQAFALPEPPALLRTSRPGLSPIWLLLVLAVAVAALLVFRSRHAGPAART
                     AGIAALPTAVPTDSATAAATLPPAPIAAATSVAPSPTGIPATPFPSTIATRVPTAIPP
                     AAAAIAVPAQPASLDRRRWMDRAQRDLRTHRRDRRTRYAIQVELVCELPSLDEAWRYD
                     RRGALWLLPADYRGRGCFRVFWGRYATLEEARAAKASVPRFFSTPTNQPAIVSTRALL
                     P"
     gene            8541..10136
                     /locus_tag="VF958_13485"
     CDS             8541..10136
                     /locus_tag="VF958_13485"
                     /inference="COORDINATES: protein motif:HMM:NF025690.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4388 domain-containing protein"
                     /protein_id="HEX9150168.1"
                     /translation="MIDPGGPSDLPADTAFRGSLEETPLPQILRRVFVQQLRGTLTLT
                     RREDVRRLYFEKGELRTATSSREGQRIGAFLRKRGRVSEDDIQWALREISRQRGSRLG
                     RKLVERGLLTRTVIDAEMKRLVEEIVFSAFEWDSGEYRFEPSAAVLDPDVVLTFSTAA
                     IIVEGIRRLPEGPVFRERLGEGGRVLRLARDPMSRYQYLPLAPQEAYVLSRVDGLLDL
                     DSLLSIAGASRMSSAKTLYALLSCGLIEWKTDAARKRETAGSIATLNVEVTNEPPPST
                     PGHAELVRNTWRRIDWLTHYDLLGLARDATADDIRRAYFDKSRLFHPDLRHRPDLAAF
                     SKELTAVFERLKVAHDTLVDPEARAEYDRAVDEPPAAIFTEERQEADPEARRQLAARN
                     FSRAMDLIDAKDFFPAVELLQEAVRFAPEKAEYRFRLGEVELKNAQWLDRGLENLKEA
                     TRLAPSRTDFLRTTARALLSHGRKREAEPFARRAADLEPGPESAALLKEITGRPTLDT
                     TAAAMAEPERRAGLLSRLLGKGR"
     gene            10162..10773
                     /locus_tag="VF958_13490"
     CDS             10162..10773
                     /locus_tag="VF958_13490"
                     /inference="COORDINATES: protein motif:HMM:NF024937.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4126 domain-containing protein"
                     /protein_id="HEX9150169.1"
                     /translation="MNALLTGLGLAAAAGFNAWAVLIVFHALYRLLPQELPGLVAPLL
                     GSQTVFGFALVLFLAEFIIDKIPLLDRLWDLAHTLLRPVVGALLALAAVPATSLLQQI
                     GIGFGGAVAAAAAHVVKSTTRLETTAATHGWTQFALSLAEDVVAVGIAALVFFVPWMT
                     ALFLVAIMVLLVTHFERVRRALAVLFFRIQHPRRRHRQKPAGA"
     gene            10783..11229
                     /locus_tag="VF958_13495"
     CDS             10783..11229
                     /locus_tag="VF958_13495"
                     /inference="COORDINATES: protein motif:HMM:NF017774.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HEX9150170.1"
                     /translation="MPYILDGDNLVGTARRRRAEAQDRDALVAEISDRLRKTRAKIVL
                     FFDGTGNALSLGNLAVRFAGAISADDAILREISRAPRPGETTVVTADRDLARRTRDAG
                     AVAVSPQDFWKRFGSVETPSARGPEETRVNVEEWLEWFSDETNRDK"
     gene            11259..11643
                     /gene="rnpB"
                     /locus_tag="VF958_13500"
     ncRNA           11259..11643
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="VF958_13500"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
     gene            11812..>12152
                     /locus_tag="VF958_13505"
     CDS             11812..>12152
                     /locus_tag="VF958_13505"
                     /inference="COORDINATES: protein motif:HMM:NF014114.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LD-carboxypeptidase"
                     /protein_id="HEX9150171.1"
                     /translation="MRELLKPPRLRQGATIGVANISGPVDPARLERGLAALRASGYRV
                     VEAENVRRRDGFLAGSDSERATGYRTLLRDPSVDAIFFARGGYGASRALGHLDPGEIR
                     SYPKIHLGGSDL"
BASE COUNT         1957 a         4187 c         4045 g         1963 t
ORIGIN      
        1 ggcgacccgc acggccgaca cgttgtacga gtattcgagc gcccgccgga acgtgacgat
       61 gccacggaac tttccgtcgt agttcgtcgg ccggtacggg ggctgcctgg ggtcgtacac
      121 gatcgaaatc ggcccgtcga agacggtgtc cgacggcgtg tacccggctt caagcgcggc
      181 caggtagacg aaaggcttga aggtcgatcc ggcctgtcgc agcgcctggg tggcccgatt
      241 gaacttcgag cgcgtccagt cgtaaccccc cgtcatcgcg cgcacggcgc cggtcgagtt
      301 ctcgatgacg agcaccgcgc cctcttcctt cgggtcgttc tcgagggaga gcaactccga
      361 tccgtccttc ctttgctcga tcgagatggc gacgaggtcg ccgcgcttca ggatcttcga
      421 agggtccgtc gcggaggtcc acgcgtatcc ggcgggaagg atcggcagca ccctcgttcc
      481 aacgcggatc tctccgcccg agcgcgaaac ggagagcacg atcccgtgca tcgctccgcc
      541 gctacctcgg gacgcgctct cccaggaggg atcgacgaac gattcgaggt ctttgtagcc
      601 ttcgtcgagc aggttgcgag gcttctggaa cccgtgccgc cgcgcgagcc tccgcaggcc
      661 ccagcggagc gcctcgtcgg accactgctg aatctgcggg tcgagagtgg actcgacgcg
      721 cagaccgcgc cggtagagct cctgctcgcc gtaggtccgc tggaggtact gtcggatctc
      781 ctcgcagtag tacggtccga cgatcgtctc ttcgagcgag ggagtggtcg gaagcggctc
      841 tccgttggcc gcccgttcgt ccgcgtcggt gatgtaaccc tccgccttca tccgcgcgag
      901 ggcagtcgcg cgccggcgcc gcgcctgcgt cgggttgcgg aacggagact ggtcttcggg
      961 gcgctgcacg atccccgcga gaagggcggc ctccgcgaga gtcaagtcct tgatgccctt
     1021 cccgaagtag tagcgcgacg ccgcctcgac cccgtagttc ccgtggccga agtagatctc
     1081 gttcgcgtac atcgtgagga tctgctcctt cgagtaacgc cgctcgatct cgaaggcgac
     1141 gaacgcctcg ttgatcttcc gggagatcgt cttgcgggga gaaagaaaga tcgcgcgcgc
     1201 gagttgctgg gtgagggtcg atccgccctg agcgaagctc ttctgtttca ggttcgccac
     1261 gagcgccgac acggtccgct ggacgtcgac gcctccgtgg cggaagaagt tcttatcctc
     1321 ggtcgcaatg atcgcattgc gaagcacggc cggaatctga ccccgggaaa cgaggattcg
     1381 ccgctcgatc gcgtagcgtg cgatcgtgga gccgtcgctg ccccggatct ccgtgatgat
     1441 gtcgggacgg tacgtctcga gctcggagac gcggggaacg cgcacgaagt tcgtcgccgc
     1501 cgaccctgcc acgacgccga ggagcgccgc cgcgagaagg ggcacgaccg cgatgacggc
     1561 cttcctcccg gtgatcacac cggcattttg aggcatcgcc gctcgctatg ctataagccg
     1621 aaggctcgtg agtacgccgc ctcccatcga acccgctccg gccgggcttc ccgcgaaccg
     1681 cggttgctgg aagggcgcgc tctacgggtg tggagcggtc gccgtatgca ttgtcgcggt
     1741 cctcgtcggc ctcgggattt acgttcagaa gaggcccggc gcgatcaccg acatgctgat
     1801 ggagagaatc cgcgaccgtt acgcctcgga cgtgacggcg caggacaagg cggacctcga
     1861 ggcggcttat gcggactttc ggaccgccct cgaagccaag cgcgtccggc gagatgacct
     1921 cgaccgcgtc cgcttcacgg tcaagctcgg acgtgaggtc cgccacgatg aggtcgtcga
     1981 gctgacccgg gtcttccggg aagcggccga aaacggggga cgagactcgg gacaggggac
     2041 ccgagaagcc atgcccgccg cgacgcctcc accggtcgcc gcaaccccgg tgccttgagc
     2101 ccgcgccccg gctcccggcc gaaaccggca ctcgcacccg ggcgcccgac cgcgaagccg
     2161 tcctcctacc tcgacgccca gtcgcgcgcc ggcggtcgat tcgcgctgaa gaccgacttc
     2221 accccgcagg gcgaccagtt gcaggccatc gatcatctcg cccggggcat cgacgagggg
     2281 cggcgcgacc aggtcctcct gggcgtcacc ggctcgggca agaccttcac gatggcgaaa
     2341 gtgatcgagg cggtcaaccg gccgacgctc gtgctctcgc acaacaagac tctcgccgcg
     2401 cagctttacc aggagttccg cgggttcttc ccggagaacg cggtcgagta cttcgtctcg
     2461 tactacgact actaccagcc cgaggcgtac atcccgcaga ctgacaccta catcgagaag
     2521 gagacgtcgc gcaacgagga gatcgacaag ctccggctct ccgcctcgaa ggctctcttc
     2581 gagcgccgcg acgtcatcgt cgttgcgtcg gtgtcgtgca tctacggcct gggctcgccc
     2641 gagtcctact acgacatgct ggcgtacgtg gaggtcggcg accgctccgg catgcgggcg
     2701 cttctcgcgc agctcgtcga gatgcagtac gagcggacga acctcgacct cgcccgcggg
     2761 acatttcgcg tgcgcgggga cgtgctcgag atccatcccg cttacgagga cgtcggggtg
     2821 cgaatcgagt atttcggaga cgaggtcgag aaagtcacgc gcacggatcc cctccgcggc
     2881 acggcgctcc agaaggttca acggatcgcg ctgtatccgc ggacgttcta cgccaccccg
     2941 cgtgagactc tcgagcgagc catcggcacg atccgactcg agctcgacgc ccgtctcgcc
     3001 gagctctcgg cggctggacg gctgctcgaa gcgcagcgtc tccaccagcg cacgatgttc
     3061 gacctcgaga tgatcaagga gctcggctac tgcaacggga tcgagaatta ctcgcggcac
     3121 ctttcgggga ggcagcccgg ggagcccccc cccacgctca tcgactactt tccgcgagac
     3181 ttcctcctcg tgatcgatga atctcacgtc actctcccgc aggtacgggg catgtaccac
     3241 ggcgatcgat cgaggaagga ggtgctcgtc gattacggct tccgcctccc gtccgcgctc
     3301 gacaatcggc cgctcacgta cgaagagttc gaggcgcggt tgaaccagat cgtctacgtg
     3361 agcgcgacgc ccggccccga agagctggga aagaccggcg gggaagtcgt ggagcaggtc
     3421 attcgaccga cgggtctgct cgatccggtc atcgaggtgc ggccggtcaa gggtcaggtg
     3481 gacgacctcc tcgccgtggt acgcgacgcg gcggcgaagt cgcagcgcgt tctggtcacg
     3541 acgctgacca aacggatggc cgaggagctc acgaactact tctcggaggt cggcgtgcga
     3601 tgccggtacc tccactccga catcgagacc ctcgatcgcg tcgcgatcct catggatttt
     3661 cgccggggtg tgttcgactg cctgatcgga atcaacctgc tccgggaggg cctcgacctg
     3721 ccggaagtgg cggtggtcgc gatcctcgac gcggacaagg agggcttcct ccgaagccag
     3781 acgtctctca tccagacggc cggccgcgca gcgcgcaacc tggaggggcg ggtcatcttc
     3841 tacgccgacg cggtaacgga ctcgatgcgc cgggccatcg aggagaccgg gcggcggcgg
     3901 aagatccagg aggcctacaa ccgcgagcac gggatcgaac cggcgtcgat cgtcaagcag
     3961 gtcaactcgc ccctcgtgcg gatgtcgagc ctcgactact acgacccgac ccccgcgctc
     4021 tcccggcccg atctgccgga cgccccctcg ctcggccgac ggattgtcga gctcgagaag
     4081 gccatgcgcg cggcggcgaa ggagctcgac ttcgagcgcg ccgcggagat ccgcgacgaa
     4141 ctgcgcgggc tgcgggagtt gcagatcttc cgttcgtgaa ggagttacgg ctgcgttctc
     4201 cgcgccgcgg cgccgccggg gtgacgcccc tcagtcgggg tttttggcag gcgccggggt
     4261 gctcgccggc ggagccgggt gcgcgagcac gaactccctc gcggcccggg ccgcgaagtc
     4321 ggcgggctcg agcgacagcc gcggctcgtc gatttcgacc gtgagctgcg gatcgaacga
     4381 gatacgcccc gagaaaccgt ccggcgacat gcgcccgtat tgcgaagcga tcagaggatc
     4441 gggcgcatag cgaatgccga aaggaatcgc gcaggggagg gcgccggagc agcgttgcag
     4501 cctcatccac tgcgcgcgcg ccgtcggagc gaaccccagg agggccgcga acccggccgc
     4561 caggaagagg cctcgagtcc gcatggtcac aacaaagata acacgcggcg tcgcgccgat
     4621 attgccgcag cgagcgggaa tgctgaggcg ggtgcaggtc cgcaggacct gtccccgcga
     4681 caattacaag aggtggcagg cccgcgcgaa tccgccgacc acgaccagca ccgcgatgac
     4741 gagcacgacg cggaccgccg tcttcttctt cgtgccgagc ggctgccgcg tgtagaaccc
     4801 ggtctgccag cgctcgatct cgcgtccgca cttcggacac accgaatcct gaggacccac
     4861 cgtcgtgtag cagcacgggc actcgttcat tgcgttgctt catcctacga caggacgaag
     4921 tcgatgtctc cgagcgcgaa tccggcgcgt tacactgccg ggcgtgagcg attcgcgccg
     4981 cccccccgag aacgtccccg acgcacccgg cgtttacctc ttcaaggatc ggcggggaaa
     5041 gaccctctac gtcgggaagg cccgctctct gaagaagcgg gtcgcgtcgt acttcgttcg
     5101 cggacccgag gtgacgaaga ccgaggcgct cctcgactcg ttcgcagaag tggagaccat
     5161 cgtcaccaac acggagctcg aagcgctctt actcgaaaac tccctcatca agaaacaccg
     5221 cccgcgctac aacatctgcc tgcgggacga caagacctac ccgtacgtca agatcaccac
     5281 cggcgaagat tggccgcggg cgctcgtgac gcgccgggtc gccgaagacg gacactcgta
     5341 cttcgggccg ttctgggggg gcctcgcgcg ccggatcatg cggatgatca cacggcactt
     5401 ccagatccga acgtgcacga tcgagatcga cggtacgctg ccgcgcccct gcctctatta
     5461 cgatcttcac gcgtgtcttg gcccctgcgt cgccgggctg accagcaagg cggcgtatga
     5521 cgaggcggtg agagatgtcg tgctcttcct tcagggacgc aaccgagagc tcctcgcgag
     5581 cctggagaag aagatgctcc aggcttcctc cgaggagaac ttcgaaatgg cgtctgctta
     5641 tcgggacgcg ctccgcacgg tcgaagacgt ctcggaacac cagatcgtgc actcgctcaa
     5701 gggggaaagc gtcgacgttt tcgggttctt cgagtcgggg ggcgatgtcg cggtgtgcgc
     5761 cctcgtcgtg cgtggaggcg tcatccagga ccggcgcgag ttcttcttcg agaagtcgga
     5821 ggaggtcgat ccaggggtct ttttcgacgc cttcctgccg cagttctacg acgcgaaccc
     5881 ctttctcccg gccgaggtgc acctgccggt cgcgatcgca gagcccgatc ttctcgaaac
     5941 attcctctcc tcgcgtcgcg gcgcgagagt ctccgttcgc gtcccccggc gtgggccggc
     6001 gcgcgagcgc gtcgagctcg cgcaggacaa cgcccgcgag cggcacaagg tgcggtttcg
     6061 acgcatggga ggggagggag cgctgggagt cgagagactc gcccggatcc tcgatctgcc
     6121 cgtacctccc cggcggatcg aggcgttcga catttcgcac ctccagggca ccgactcgat
     6181 cgcctctctc gttgtgttcg aggacgggaa gccgaagaag tccgattacc ggctgttcgg
     6241 gatcgcctcc caggagcttc ttgcccccga cgactttcgc agcatggccg aagccgtcga
     6301 gcgccgatac cgcaaggtgc gcgacgaagg gatcgaggtg ccggatctcg tcctcgtcga
     6361 cggggggcgt gggcagctcc aggcggcgct gacggcgctc gatcggctgg gcgtcgagct
     6421 gccggtcgtg ggtctcgcga agagggagga agagatctgg gtgcccgggc gccccgaccc
     6481 gatccggctc ccccggaaag acccggccct ccagctcgtt cagcgcgtgc gcgatgaagc
     6541 gcaccggttc gcgatcacgc gacaccgcgg gcggcgatcg aagcgcatgc gcgagacgag
     6601 cctcatggaa atcccgggga tcggaccgac ccgcgctcga acgctacttc gcaggttcgg
     6661 atctctctcc ggactggccg cggcggaccc gaaggacgtc gaaacggccg tgggtcccgc
     6721 ttcggcgcgc gcggtccgcg agtatctcca ggcggcgcgc cgccaggacg agccggcggc
     6781 actcccgggc ggcctcggcg agggagggcg cgtccgctcg gcatgagaac cgccttcgag
     6841 cgtgagggcg acatccggag cgtcgacctg accggcgcgc tcgcggcact gtggcaggaa
     6901 cgggccacag gagttctcgc gtttgcgagc ggcgaggagc gcgtccgatt cgaaatcctg
     6961 gagggcgagc tcgtcggggc gttctcctcc gatccttcgt tcgacacagc agaggtcctc
     7021 atacgcggcg gtaagctcga tggcggggcg ctcgagggcc gtcagttccc gcccgggcgt
     7081 gaccgcgccc gtgccgctcg ggagctggga gttctgaccg agcgcgactg gcgctgggga
     7141 gaaaagatcc gggcggtcga gatcctctcg gatctgatcg gttggctcga cggtcgctat
     7201 tccttcgatc ccggcgagcg cccggcggcc ggggacttcc aactcggaat cgagaggctg
     7261 ctcctcgagc tcttcctgcg ctctcgtgac cgggagttcg tccatcgatc cctgggcgcc
     7321 gtggacgctc cgctgcaacg tgccgacgac ttcgaggacc ggttccgcag tctcggcctg
     7381 accaccgacg cggagctcgt ggtggcggtt atcgacggcc gcgcgaccgc cgcggagatc
     7441 tcgcgccgcg tggggccgga cccgttctcc gtcgaaaaac tgctcgccgc gctgaccacg
     7501 ctcggcctcg tgcacccgga atatgccgct ccgccaaaag cgaagcggtc cggcccggcg
     7561 aaggtcccgc ctgcggcgct cccggccccg gtggccgagc cggagccgcc ccgtccagag
     7621 ccggaaaccc cggcgcaggg cgcgacttcc gtgcccgaag cagtcgcgcc ggccgcgccg
     7681 gcgccgcccg tcgagtcccc gtcaaacgag gtcgaaccgc tcgcggaggc gttcgaggag
     7741 ccggttctcg ggccggagcc cgtcgaactg ccgctcctcc ccgtaccgga accaacggag
     7801 acggaaccgg tcctgacgag ctgggagccg atccctccgg aaccgctcga tcaggcgttc
     7861 gcgctgccgg agcccccggc cctcctgcga acgagtcggc cgggcctgtc gccgatctgg
     7921 ctgctcctgg ttcttgccgt cgcggtcgca gcgctcctcg ttttccgctc gcgccacgcg
     7981 ggaccggccg cccgcaccgc cggcatcgca gcccttccaa ccgccgtgcc gacggattcg
     8041 gcgactgccg ctgcgaccct cccgcctgcg ccaatcgcgg cggcgacctc cgtcgcgccg
     8101 agtccaaccg ggatacccgc aacgcccttc ccctcgacga tcgcgacgcg cgtgccgacc
     8161 gcgattcctc cggccgccgc cgcaatcgcc gtccccgccc agccggcgtc gctcgaccgc
     8221 cggcgctgga tggaccgcgc gcagcgtgac ctccgaactc accgacgtga ccggcggacc
     8281 cgctatgcga tccaggtcga actcgtctgc gagcttcctt cgctcgacga agcgtggcgc
     8341 tatgaccgcc ggggcgccct ctggcttctc cctgccgact accgtgggcg ggggtgcttc
     8401 cgcgttttct gggggcgata cgccacgctg gaagaggcgc gcgcggcgaa agcgtcggtc
     8461 ccgaggtttt tctccactcc gaccaaccag ccggcgatcg tctcgacgcg cgctctgcta
     8521 ccctgaacgc acaagcaacg atgatcgacc ccggcggtcc ctcggatctc ccggccgata
     8581 ccgcgttccg cggctcgctc gaagagaccc ctctgcccca gatcctgcgg cgcgtcttcg
     8641 tccagcagct gcgaggcacg ctcacgctca cccgacgaga agacgtccga cggctctact
     8701 tcgagaaggg cgagttgcgc acggcgacgt cttcccgcga aggccagcgt atcggcgcgt
     8761 tcctccggaa gagagggcgc gtttccgaag acgacatcca atgggccctg cgtgagatct
     8821 cgcgacagag aggatcgcgg ttggggcgga agctcgtcga gcgcggcctc ctcacacgaa
     8881 cggtgatcga cgccgagatg aagcgtctcg tcgaagagat cgtgttctcg gcgttcgagt
     8941 gggactcggg agagtaccga ttcgagcctt cggccgcggt tctcgacccc gacgtggttc
     9001 tgaccttttc gaccgctgcg atcatcgtcg aaggaatccg gcgcctaccg gaagggcccg
     9061 tcttccgcga aagactcggt gagggcggcc gagtcctccg cctggctcgg gacccgatgt
     9121 cgcggtatca atatctgccc cttgcgccgc aggaggcgta cgtcctctcc cgggtcgacg
     9181 gactgctcga cctggattct ctcctctcga tcgccggagc ctcgcgcatg tcctcggcga
     9241 agaccctcta cgcgctcctc tcgtgcggcc tcatcgaatg gaagaccgac gccgcccgga
     9301 agcgcgagac ggcgggtagc atcgcgacgc tcaacgtgga agtcacgaac gagccgccgc
     9361 cgtccactcc ggggcacgcg gagctcgtcc gcaacacgtg gcgccgcatc gactggctga
     9421 cccactacga tctcctcggc ctggctcggg acgcgaccgc ggacgacatt cgccgcgcct
     9481 acttcgacaa gagccgcctc ttccatcccg atctgcgcca ccgccccgac ctggccgcgt
     9541 tctcgaagga gctcacggcc gtcttcgagc gcttgaaggt cgcgcacgac acgctcgtgg
     9601 atcccgaggc gcgcgcggag tacgaccggg cggtcgacga acccccggca gcgatcttca
     9661 cagaggaacg acaggaggcg gatccggaag cgcggcgcca gctcgcggcc aggaacttct
     9721 cgcgcgcgat ggacttgatc gatgcgaagg acttttttcc cgcggtggag ctcctgcagg
     9781 aggctgtccg cttcgctccc gagaaggcgg agtaccgctt tcgcctcgga gaggtcgagc
     9841 tcaaaaacgc ccagtggctc gatcgcggcc tggaaaacct gaaggaggcg acgcgcctcg
     9901 ccccgtcgcg gaccgacttc ctgcgcacga cagcacgtgc gctcctttcc cacggccgca
     9961 agcgcgaggc cgagcccttt gcccgacgcg cggccgacct cgagccggga cccgagagtg
    10021 ccgcgctcct gaaagagatc acgggccgcc cgacgctcga tacgacggcg gccgcgatgg
    10081 cggaaccgga acgacgcgca gggcttctct cgcgcctcct cggaaagggg agatgatgcg
    10141 gagccgggag attcgaccgg cttgaacgct ctcctcaccg gcctgggact cgcagctgcc
    10201 gccggcttca acgcgtgggc ggtgctgatc gtgttccacg cgctctaccg acttcttccg
    10261 caggagctcc ccggcctggt tgcgccgcta ctcggcagcc agaccgtatt cggtttcgcg
    10321 ctcgttctct tcctcgcgga gttcatcatc gacaagattc cactgctcga tcgcctttgg
    10381 gatctcgcgc acactctcct tcgtccggtc gtcggcgcgc ttctcgcgct cgcggcggtc
    10441 cccgcgacat cgctccttca gcagatcggg atcggcttcg gaggggcggt cgcggcggcg
    10501 gccgcccacg tggtcaagtc cacgacccgc ctcgagacga cggcggcgac gcacgggtgg
    10561 acgcaatttg cgctctcgct cgcggaggac gtcgtggcgg tcggaatcgc ggcgctcgtc
    10621 ttcttcgtgc cgtggatgac ggcgctcttc ctcgtggcga tcatggttct cctcgtcacg
    10681 cacttcgagc gggtgcgaag ggccctcgcg gttctgttct tccgcatcca gcacccccgg
    10741 cggcggcacc ggcagaagcc cgcgggcgcc tgacggccgg cggtgccgta catcctcgac
    10801 ggcgacaacc tcgtcgggac cgcccgccgc cggcgcgccg aggcccagga ccgcgacgcg
    10861 ttggtcgccg agatttccga ccgactccgg aagacgcgcg cgaaaatcgt cctcttcttc
    10921 gacggcaccg gcaacgcgct ctcgctcggg aatctcgccg tgcgtttcgc gggagcgatc
    10981 tcggcggacg acgcgatcct gcgggagatc tctcgcgctc cccggcccgg cgagacgacg
    11041 gtcgtcacgg ccgatcgcga cctcgcccgt cggacccgtg acgccggggc ggtggcggtt
    11101 tctccccagg acttctggaa acggttcggt tccgtcgaga ccccctcggc gcgcggcccg
    11161 gaggagacgc gcgtgaacgt cgaagagtgg ttggagtggt tctcggacga gaccaatcgc
    11221 gacaaatagc tcgctcacgg tgctatgctt gtgtcgtccg agcgggctga gcgatcgccc
    11281 cgggaagccg gggaggaaag tccgaactcc gaacgggcgg catgccgggt aatgcccggt
    11341 cgcggcgacg cgaaggaaag tgccacagag aaaagaccgc ccttcccggg cagacgtccg
    11401 gggacggtaa gggtgaaacg gtgcggtaag agcgcaccgc cccgccggca acggcggggg
    11461 cacggtaaac cccatgcgga gcaaggccaa atagggagat ggagggcgcc cgtcccgtcc
    11521 cgcttttcgg cgggccgtct ccgggtcggc tgctcgagcc cgggggagac cccgggccga
    11581 gaggaatgat cgcgcttccg ggagaccggg agacagaatt cggcttacag gcccgctcgg
    11641 acttttccat cgcgctcgct tgctcggcat ctggctgcgc caccggcgct cggctcggca
    11701 gaccggcctc gaccgagcgc ctgatgcctc gcagcactcg cgctcccgca tcgcgaagct
    11761 cctccgctcg gttacttcgc ggagactctt cgcgatgcga acggaggctc attgcgtgag
    11821 ctcctgaagc ctccgcgcct ccggcagggt gccacgatcg gcgtcgcgaa tatttcggga
    11881 cccgtcgacc ccgcgcgcct cgagagaggt ctcgccgcgc tgcgagcgag tggttaccgc
    11941 gtcgtcgagg cggagaacgt gcggcgccgc gacggatttc tcgcggggag cgactccgag
    12001 cgtgccactg gatacagaac tctccttcgg gatccgtccg tcgacgcgat cttcttcgcg
    12061 cggggggggt atggggcctc gcgcgctctc gggcacctcg atcccggcga gattcgttcc
    12121 tacccgaaga ttcacctcgg cgggtcggat ct
//
LOCUS       DASWAV010000646         3164 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U2665
            k141_639707, whole genome shotgun sequence.
ACCESSION   DASWAV010000646 DASWAV010000000
VERSION     DASWAV010000646.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960316
            Sequence Read Archive: SRR10260007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 3164)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3164)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:47:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,929
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3164
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_639707"
                     /isolate="SMAG_U2665"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.36444 N 132.31889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..629)
                     /locus_tag="VF958_13510"
     CDS             complement(<1..629)
                     /locus_tag="VF958_13510"
                     /inference="COORDINATES: protein motif:HMM:NF014458.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HEX9150172.1"
                     /translation="MLSREFLRASAGRLLSEFPDRFANSGVERFLEVDGRRRGVVTRL
                     EEKRRRRNELTAVRGKPDPGTIAEMKSLKEEIRGLEEEAEKAEAELSNVERGIPNVPN
                     DSVPRGADEKSNRVERTWGEPRKFDFEPAAHWDLGPALGVLDFERGAKLAGARLSRAL
                     GNFMLAIHSREHGYEEVVPPLLNNAETLFGTGQLPKFEADLFRTREGLYL"
     gene            701..1636
                     /locus_tag="VF958_13515"
     CDS             701..1636
                     /locus_tag="VF958_13515"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEX9150173.1"
                     /translation="MDCVVVNRDGDQPLFDLLGSLERQTGIDLSVIVVDNGSRESERE
                     RLARDAPAARVVSFSRNLGFAAAANEGIARTRSPYVLVVNNDAVLAPDYAARLAARLA
                     LDERLSAVQGLVLERRGERVDTAGIEWNDRGEAVPVFGGLDAKRAPKDAFEVPGVSAT
                     AVLFRREALVDVAPHGHIFDDRFFAYYEDVDLSLRLARAGWRFACDPAAIAWHEGSRT
                     GRRTPLRRALWTARNRWRTLYGNFERRWIRRNLVPLLRADLAHARTLGWRGVIVPLLV
                     WPRIPFVALTSRAGPRLLSSWPTAGRSGAPVAMRA"
     gene            1633..2619
                     /locus_tag="VF958_13520"
     CDS             1633..2619
                     /locus_tag="VF958_13520"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX9150174.1"
                     /translation="MSRTNPITAILVSWKDADETLQAVESLAQARALVPPDGPRVSLV
                     VVDNDGGTLDRSALLSRWPDATLVVNEKNRGLGPAVNQATKIAGEGLLLLVNPDTRAL
                     GEPFGEIARAFDENPRAVAVAPRLVDDAETTGRLAPPGREDQFTFQLRRLPRLSDDAR
                     GLLLFDHVAPDNRWRRRARYADRDPDAPLEVEQPAAAALAVRGDAFRAVGGFDEIFVP
                     AWFEDVDLCARLLERGPIVYWPAAKFRHRGGAASQELGYARFLPAYYQNAILYRRRRY
                     GVAARLGYRALLAAGMVLRLAALPVRRSPRPRRESAVAYVKTLGVALGLTRR"
     gene            2616..>3164
                     /locus_tag="VF958_13525"
     CDS             2616..>3164
                     /locus_tag="VF958_13525"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX9150175.1"
                     /translation="MTPDVSIVVVSHESAGDLPLSLASAVAQAGVAVEILVVDNASRD
                     GSRDVAARSAPEARVLALPANAGFAGGMNAGIDATSGRYVLSLNPDCRLAPDFAATLV
                     RRMDARADAGSATGRIYRARGRDLAPTSRLDSTGIVFTASGRHFDRGSDEEGEGRYLE
                     EEEVAGTTAAAGLYRREALESAK"
BASE COUNT          410 a         1158 c         1096 g          500 t
ORIGIN      
        1 agatacagcc cctcgcgggt tcgaaagagg tcggcttcga acttcggcag ctggcccgtg
       61 ccgaagaggg tctccgcgtt gttcaggagc ggcggcacga cttcctcgta cccatgctcc
      121 cgcgaatgga tcgcgagcat gaagtttccg agtgcccgcg agagccgcgc gccggcgagc
      181 ttcgcgccac gctcgaagtc gaggacgccg agggcggggc cgaggtccca gtgcgccgcg
      241 ggctcgaagt cgaacttccg cggctcgccc cacgtgcgct cgactcggtt gctcttttcg
      301 tccgcgcccc ggggcacaga gtcattgggg acgttcggaa tgccgcgttc gacgttggag
      361 agctccgcct cggccttctc tgcttcctct tcgaggccgc ggatctcctc cttcaaagac
      421 ttcatttcgg cgatcgtccc ggggtccggc tttcctcgga cggcggtgag ctcgttgcgg
      481 cggcggcgct tctcctcgag gcgagtgacg acgcctcgtc ggcggccgtc cacctcgagg
      541 aagcgctcga ctccggagtt cgcgaaacgg tccgggaact ccgagagcaa ccgcccggcg
      601 ctcgcgcgga gaaactcgcg tgatagcatg agttgcgcga tttcatcccg cgcgggcaga
      661 cgagtcaaca gctttgagcc cggctaactc ccttccagac gtggattgcg tcgtcgtcaa
      721 ccgggacggc gaccaacctc tcttcgatct cctcgggtcg ctcgagaggc agaccggcat
      781 cgatctgtcg gtcatcgtcg tcgacaacgg ctcgcgcgaa tcggaacggg agcggctcgc
      841 ccgcgacgcg ccggcggcgc gcgtcgtctc gttctcgcgc aacctcggct tcgccgcggc
      901 ggccaacgag ggaatcgcgc ggacccgctc gccctacgtg ctcgtcgtca acaacgacgc
      961 ggtcctcgcg cccgactacg cggcgcgcct cgccgcgcgt ctcgccctcg acgaacgact
     1021 ctcggccgtc cagggtctcg tcctcgaacg gcgcggggag cgcgtcgaca cggccgggat
     1081 cgagtggaac gaccgcggcg aagcggtccc ggtgttcggc gggctcgatg cgaagagggc
     1141 tcctaaagac gccttcgagg tcccgggggt ctccgcgacg gcggtcctgt tccgccgcga
     1201 ggcgctcgtc gacgtcgcgc cgcacggcca cattttcgac gaccggtttt tcgcgtacta
     1261 cgaggacgtc gacctgtcgc tgcgcctggc gcgcgcgggc tggcgcttcg cctgcgatcc
     1321 cgcggcgatc gcgtggcacg agggctcgcg caccgggcgc cgcacgccgc tgcgccgggc
     1381 gctctggacg gcgcgcaacc gctggcggac tctctacggg aacttcgagc ggcgttggat
     1441 ccggcggaac ctcgtgcccc tgcttcgcgc cgaccttgcc cacgcccgaa ccctcgggtg
     1501 gcggggcgtg atcgtcccgc tcctcgtctg gccgcgaatc cccttcgtcg cactcacgtc
     1561 gcgcgcgggc ccgcgccttc tctcgagttg gcccacggcg gggcggagcg gcgcaccggt
     1621 cgcgatgcgg gcgtgagtcg cacgaacccg atcacggcca tcctcgtttc ctggaaggac
     1681 gccgacgaaa cgctccaggc ggtcgagtcg cttgcgcagg cccgtgccct cgtgccgccg
     1741 gacggtccgc gcgtttccct cgtcgtcgtc gacaacgacg gtgggacgct ggaccgaagc
     1801 gctcttctct cgcgctggcc cgacgcgacg ctcgtcgtca acgaaaagaa ccgcggactc
     1861 gggccggccg tcaaccaggc gacgaagatc gccggcgagg gcctgctcct gctggtcaac
     1921 cccgacacgc gcgcactcgg ggagccgttc ggcgagatcg cccgcgcctt cgacgagaac
     1981 ccgcgcgccg ttgccgttgc gccgcggctc gtcgacgacg cggagacgac cggccgtctc
     2041 gccccgccgg gccgcgagga tcagttcacg ttccagctgc gccggctgcc gcggctctcg
     2101 gacgacgcgc gcggacttct cctgttcgat cacgtcgctc ccgacaaccg ctggcggcgc
     2161 cgggcccgct acgcggaccg ggatcccgac gcgccgctcg aagtcgagca gccggccgcc
     2221 gcggcgctcg ccgtgcgggg cgacgctttc cgggcggtcg gcggcttcga cgaaatcttc
     2281 gtgcccgcct ggttcgagga cgtggatctc tgcgcgcgcc tcctcgagcg ggggccgatc
     2341 gtctactggc cggcggcgaa attccgccac cgcggcggcg cggcctcgca ggagctcggg
     2401 tacgcgcgct tcctgccggc gtactaccag aacgcgatcc tctatcgccg ccgccgatac
     2461 ggcgtcgcgg cgcggctcgg atatcgcgct ctcctcgcgg ccggaatggt cctgcgcctc
     2521 gcggcgcttc ccgtccgccg gtcgccgcgc ccgcgacggg aatccgccgt cgcctacgtg
     2581 aagactctcg gcgtggctct cgggctgacg cgccgatgac ccccgacgtt tcgatcgtcg
     2641 tcgtctcgca cgagtccgcc ggcgatctgc cgctgtcgct cgcttcggcg gtcgcccagg
     2701 ccggcgtcgc tgtggagatc ctcgtcgtgg acaatgcctc tcgcgacggg agccgggacg
     2761 tcgccgcgcg ctccgcgccg gaggcccggg tgctcgcact gcccgcaaac gcgggtttcg
     2821 cgggggggat gaacgccggc atcgacgcca cgtcggggcg gtacgttctc tcgctcaatc
     2881 cggactgccg ccttgcgccc gacttcgcgg cgactctcgt ccgccggatg gacgcgcggg
     2941 ccgacgcggg ctcggcgacg gggcgaatct accgcgcccg ggggcgtgac ctcgcaccga
     3001 cgtcgcgtct cgattcgacc gggatcgtct tcaccgcttc gggccgccac ttcgaccgcg
     3061 gaagcgacga agagggagag gggcgctacc tcgaagaaga ggaggtggcg ggaacgacgg
     3121 ccgccgccgg gctttaccgc cgcgaagcgc tcgagtcggc gaag
//
LOCUS       DASWAV010000647         6588 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U2665
            k141_478622, whole genome shotgun sequence.
ACCESSION   DASWAV010000647 DASWAV010000000
VERSION     DASWAV010000647.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960316
            Sequence Read Archive: SRR10260007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 6588)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6588)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:47:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,929
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6588
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_478622"
                     /isolate="SMAG_U2665"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.36444 N 132.31889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(145..231)
                     /locus_tag="VF958_13530"
     tRNA            complement(145..231)
                     /locus_tag="VF958_13530"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(196..198),aa:Leu,seq:caa)
     gene            383..904
                     /locus_tag="VF958_13535"
     CDS             383..904
                     /locus_tag="VF958_13535"
                     /inference="COORDINATES: protein motif:HMM:NF044704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CarD family transcriptional regulator"
                     /protein_id="HEX9150176.1"
                     /translation="MGFRVGEKVVYPNHGVSVVEKIGEGFITDAAPVPPRSFYHLRLL
                     ANNSKVMVPIGNTDLIGLRRLTQRKDITGLMKTLSDGRFSATGDWKGRYKQNLDKMKS
                     GRLHDIADVLKTLNFIMTRKTLSFREKKMYERAKYLIVSEIAVINSMDEGEVERLVDR
                     ALAKSAQSRAAGH"
     gene            985..1716
                     /locus_tag="VF958_13540"
     CDS             985..1716
                     /locus_tag="VF958_13540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9150177.1"
                     /translation="MRRISLLTIVMTVVAGSLLADTVSDVLRADLARERRRMAVDASQ
                     LADVGRRLDTATGDLATASRAVSDLAARADSSVDEIARREDAVASAEQEVRTLLDRRR
                     LLVERLLERRRSVALLEAELRGKRPASAAPDLLSGRWNVALDPGEQRGVFRLALDGTL
                     VSGEYAIEGGFSGSLRGTLIGDRLRLERVDSQLGFTTIYYGRLARDGREISGTWESTT
                     FGVGAAGAGRWRAQPIKEEEPEASP"
     gene            1713..2270
                     /locus_tag="VF958_13545"
     CDS             1713..2270
                     /locus_tag="VF958_13545"
                     /inference="COORDINATES: protein motif:HMM:NF025910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX9150178.1"
                     /translation="MTTTRRFPILPLLVGALLGFIGGYFAGGGGRPAPTPAAAKAGAA
                     AGEGGAGRIEELESAVARDRENPKLLTALGNAFYDREDWDRAIDAYEKARRKAPNDAN
                     LLSDLGAAYRNRGEFKRAVALFERARQADKEHWQSLLNLVLINAYDLHDSAAAQRWFD
                     ELKRRYPEIPDLDRIQERISSLRAS"
     gene            2267..2515
                     /locus_tag="VF958_13550"
     CDS             2267..2515
                     /locus_tag="VF958_13550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9150179.1"
                     /translation="MIRFALYLALVFFVLGLLRIARILLASRRGGGRASAHPVENEMV
                     RDPVCGTWIDRRLALAGTRGGEAVPVCSERCRRTLEAR"
     gene            2512..3867
                     /locus_tag="VF958_13555"
     CDS             2512..3867
                     /locus_tag="VF958_13555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013275485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HEX9150180.1"
                     /translation="MSGLLFGREDGERPGAATAEGVPLADRMRPRSLDEVEGPDEVVG
                     PNGFLFRAIAEDRVPSLIFWGPPGVGKTTLARLIAAQTSSRFLAYSAIATGVKEMREV
                     LEASRALRAKAGQRTILFLDEIHRFNRAQQDVFLPYVETGDVILIGATTENPSFELNG
                     ALLSRSKVVVLAPLSPEAIVRIVRRAIEDIPRGLGSRDFRVDDEALSFIGETAGGDAR
                     RALNLLEAAAADAETSKTRAIGVPRLRDLLQRKVLLYDKAGEEHYNLISALHKSMRES
                     DPDATVYWMVRMLEAGEEPLYLARRIVRFASEDVGLADPRALRVSLDAKEAYDFLGSP
                     EGELALVEAAVYCALAPKSNALYLAEGEAKRDVAERPAEPVPLVIRNAITRLMKDVGY
                     GRGYRYAHAEPEGVGGIECLPDRLKGTRYYRPTNSGEEAELARRLEAVLEKRREKAGN
                     R"
     gene            complement(3915..5435)
                     /gene="guaB"
                     /locus_tag="VF958_13560"
     CDS             complement(3915..5435)
                     /gene="guaB"
                     /locus_tag="VF958_13560"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015898348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003938 - IMP dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HEX9150181.1"
                     /translation="MPARRRPSGRSSDGLPLALTFDDVLLLPARSRVHPRDVDLKTRL
                     TDTVALNIPIISAAMDTVTESRLAIAMAQQGGLGVIHKNLPIPAQAEEVDRVKRSESG
                     MIVDPVTVRPEQKIAHALELMARYKISGVPVIDEKGKLVGILTNRDLRFETHLDRPIS
                     SRMTKERLVTTPVGTTLEQAKELLHKHRIEKLLVVDRHGNLKGLITVKDIQKAIKYPD
                     AAKDRLGRLRVGAAVGVSRDLLDRASELVRAQVDVLVLDSSHGHSQNVLDALKLLRKR
                     FASTPIIAGNVATESGARDLMRLGADAVKVGIGPGSICTTRVVTGAGVPQIHAIRECA
                     RATRGTRVPLIADGGIKFSGDVVKALAAGASTVMIGSLLAGTEESPGETILYQGRTFK
                     AYRGMGSLAAMKSGSSDRYFQESMEVEASTDGSLSKLVPEGIEGMVPYKGSVEGLVHQ
                     LTGGLRSGMGLTGARTVDDLRTKSTMIRVTSAGLKESHPHDVIITKEAPNYRREET"
     gene            complement(5435..>6588)
                     /locus_tag="VF958_13565"
     CDS             complement(5435..>6588)
                     /locus_tag="VF958_13565"
                     /inference="COORDINATES: protein motif:HMM:NF019155.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="SEC-C metal-binding domain-containing protein"
                     /protein_id="HEX9150182.1"
                     /translation="KSPDEFTTMLATIRAEAARDRETVLAAGGLHILGTERHESRRVD
                     NQLRGRSGRQGDPGSSRFYLSLEDDLMRIFGSDRLQNVMRKLGMEEGVPIEHGMVSRA
                     IEKAQKQVEGRNFETRKHLLEYDDVMNRQREEIYGLRRDILKGKQSKDEILGLAEVIL
                     DYAIGRSLPEDKAPDEWDLPAFDLDLKEYFGIAPGSIDFSGKERDEIRETLLSAIHEI
                     YESKEKLLGPETMRLHERFVMLQVVDQQWKDHLLAIDHLKEGIGLRGYGQRDPLVEYK
                     KESFELFTLMKERIEDQIVQYLFRLQPVIREAEGELVDEEARHAPVPMPSRRSPNVNY
                     SYGAAASGGSDAKVETIHRAGPKVGRNDPCPCGSGKKYKKCHGAAAAGA"
BASE COUNT         1034 a         2155 c         2233 g         1166 t
ORIGIN      
        1 gactcgcatc gttttctcgc gaaaacgcgc agcgggagaa gaagtttgcc cacctgcttc
       61 gcgacgcacc gcccgcggcg aagaaggatc ctcccggatc gcgaaagcgc ggctgaagcg
      121 cgatcgcttc gcgcggacgc cgtttggtgc cggaggtggg agtcgaaccc acatgcccgt
      181 agagagcggg ggattttgag tcccctgcgt ctgccattcc gccactccgg ccggcgaggc
      241 gatgataacc gtggggcctg cggcggatgc agggccgcag gtcctgccct cgcgacatcc
      301 ccggaaacat tgagtttttc gcgaatttcc tctataattc gagcttccgc agttctttat
      361 cttttgtttt gaggaggttt cagtgggatt tcgagtcgga gagaaggtcg tctacccgaa
      421 tcacggcgtt tccgtggtgg aaaagatcgg tgagggcttc atcacggacg cggcccccgt
      481 tcctccccgc agcttctatc acctgcgtct tctggcaaat aactccaagg tcatggttcc
      541 catcggcaac acggacctga tcggcctgcg gcggctcact cagcgcaagg acatcaccgg
      601 actcatgaag acactgtcgg acggccgatt ctcggcgacc ggggactgga agggacgcta
      661 caagcagaac ctcgacaaga tgaagagcgg caggctgcac gatatcgccg acgtcctgaa
      721 gaccttgaac ttcatcatga cgcgcaagac cctgtccttt cgcgagaaga agatgtacga
      781 gcgcgccaag tacctcatcg tgtccgaaat cgcggtcatc aacagcatgg acgaagggga
      841 ggtcgagcgg ctcgtggacc gggcgctggc caagtccgcg cagagtcgcg ccgccgggca
      901 ctgaatcggc gttccctgtt gagagaccgg aaaaatcggg aggcttcggc ctccttttct
      961 ttttcgcggt ctagaatcgg cgcgatgagg cgcatctccc tgctcactat tgtcatgacc
     1021 gtcgtcgccg ggtcgcttct cgcggatacg gtcagcgacg tgctccgggc cgacctggct
     1081 cgcgagcgcc ggcgcatggc ggtggatgct tcccagctcg ccgacgtcgg ccggagattg
     1141 gacacggcca cgggcgacct cgcgaccgcg agccgcgccg tttcggacct cgccgcgcgc
     1201 gccgactcct ctgtcgacga aatcgcccgg cgagaggacg cggtcgcgtc cgccgagcag
     1261 gaagtgcgca ctctcctcga ccgacggcgt ttgctcgtcg agcgtctcct cgagaggcgg
     1321 cggagcgtcg ctctcctgga ggccgaactc cgcgggaagc ggccggcctc cgccgctccc
     1381 gacctcctgt ccgggagatg gaacgtcgcg ctcgatccgg gcgagcaacg cggtgtgttc
     1441 cggctggctc tcgacgggac gctcgtctcg ggcgagtacg cgatcgaggg tgggttctcc
     1501 ggttctcttc gcggaacact gatcggagac cggttgcgcc tggaacgtgt cgattcacag
     1561 ctcgggttca cgacgattta ctacggacga ctcgcccggg acggaagaga gatttccgga
     1621 acttgggagt cgacgacgtt cggtgtgggc gctgcgggag cggggcgctg gcgggctcag
     1681 ccgatcaaag aagaggaacc ggaggcgtct ccatgaccac gacacgtcgg tttccaatcc
     1741 tgccgcttct cgtcggagcc cttctcggct tcatcggcgg ctatttcgcg ggagggggcg
     1801 gccgtcccgc tccgacgccc gcggcagcga aggcgggcgc tgcggccggg gaggggggcg
     1861 ccggacggat cgaggagctc gagagcgccg tcgcccggga ccgtgagaac ccgaaactcc
     1921 tcacggcgct cggcaacgcc ttctacgacc gggaagactg ggaccgcgcg atcgacgcgt
     1981 acgagaaggc gcgccggaag gctcccaacg acgcgaacct gctctcggac ctcggcgccg
     2041 cgtaccgcaa ccggggggaa ttcaagcgcg ccgtcgcgct tttcgagcgt gctcgacagg
     2101 ccgacaagga acactggcaa tccctgctga atctcgtcct gatcaacgca tacgacctgc
     2161 acgattctgc cgcggcgcag cgctggttcg acgagctgaa gcgacgctac ccggaaattc
     2221 cggacctcga tcgaattcag gagcggatct cgagcctccg cgcctcgtga tccgtttcgc
     2281 gctctatctc gctctcgtct tcttcgtcct cgggctcctg cgcatcgccc ggatcctgct
     2341 cgcgagccgt cgaggcggag gacgcgcttc cgcgcatccg gtcgagaacg agatggtgcg
     2401 cgatcccgtg tgcggaacct ggatcgaccg ccggctggcg ctcgcgggaa cccgcggggg
     2461 ggaggcggtg ccggtctgct cggagaggtg ccgccggacg ctcgaggcgc ggtgagcgga
     2521 ctcctgttcg gaagggaaga cggggagcgc ccgggggcgg ccaccgccga aggggtgcct
     2581 ctcgccgatc gtatgcgccc gcggtcgctc gacgaggtgg aaggtcccga cgaggtcgtg
     2641 gggccgaacg gatttctctt ccgcgcgatc gccgaagacc gcgtgccttc gctcattttc
     2701 tgggggcccc ccggggtggg caagacgacg ctcgcgcggc tgatcgccgc ccagacgtca
     2761 tcgcgctttc tcgcgtactc ggcgatcgcg accggcgtga aggagatgcg ggaagtcctc
     2821 gaggcgtcgc gggcgctccg tgcgaaggcg gggcaacgga cgatcctgtt tctcgacgag
     2881 atccaccggt tcaaccgggc acagcaggac gtcttcctgc cgtacgtcga gaccggcgac
     2941 gtcattctca tcggtgcgac gaccgagaat ccgtcgttcg agctgaacgg cgcgctgctc
     3001 tcgcgctcga aggtcgtcgt cctggctccg ctttcccccg aggcgatcgt gcgaatcgtg
     3061 aggcgggcca tcgaagacat cccgcgcgga ctcgggtcgc gcgacttccg ggtggacgac
     3121 gaggcgctgt cgttcatcgg agagacggcg ggtggagacg cgcggcgcgc gctgaacctt
     3181 ctcgaagcgg cggcggcgga cgcggagacg tcgaagacgc gcgcgatcgg cgtcccgcgg
     3241 ctgcgcgacc tcctgcagcg caaggtcctc ctctacgaca aggcgggcga ggagcactac
     3301 aacctgatct ccgcgctgca caagtcgatg cgcgagtcgg acccggatgc gaccgtttac
     3361 tggatggtgc ggatgctcga ggcgggggag gagcccctct acctcgcgcg gcggatcgtg
     3421 cggttcgcct cggaggacgt cgggctcgcc gacccgcggg ccctccgcgt ttcgctcgac
     3481 gcgaaggagg cctacgactt cctcgggtcg cccgaggggg agctggcgct cgtcgaggca
     3541 gccgtgtact gcgcgctcgc tcccaagtcg aacgcgctgt atctcgccga gggggaggcg
     3601 aagcgggacg tcgccgagcg gccggccgag ccggttcccc tcgtcatccg caacgccatc
     3661 acgcggctca tgaaggacgt cggctacggg cgcggctacc gctacgcgca cgccgagccg
     3721 gagggggtcg gcgggatcga gtgcctgccg gatcgcctca agggaacgcg gtactaccgc
     3781 ccgacgaatt ccggtgaaga agccgagctg gcgcggcgcc tggaagcggt gctcgagaaa
     3841 cgacgcgaga aagcgggtaa tcgttagtcg gattgggaat cgagagactt tcggtttccg
     3901 gtttccgttc ccggctaggt ctcctcgcgt ctgtaattcg gcgcttcctt cgtgatgatc
     3961 acgtcgtgcg ggtgcgactc cttcaagccc gccgaggtca cccggatcat cgtcgacttc
     4021 gtgcgcaggt cgtcgacagt gcgggcgccg gtcaggccca tcccgctgcg caggccgccg
     4081 gtcagctggt ggaccagccc ttcgacgctc cccttgtagg gcaccattcc ctcgatcccc
     4141 tcgggaacca gcttcgagag cgacccgtcg gtcgacgcct cgacctccat ggactcctgg
     4201 aagtagcggt cggacgatcc ggacttcatc gcagcgagcg accccatccc gcggtaagcc
     4261 ttgaaggtgc ggccctggta caggatcgtc tcgcccggcg actcctcggt ccccgcgagc
     4321 agcgaaccga tcatcacggt cgaggcgccg gcggccagcg ccttgacgac gtcgccggag
     4381 aacttgatgc ccccgtccgc gatgagcggt acgcgggttc cgcgggtcgc ccgggcgcac
     4441 tcccggatgg catggatctg cggcacgccg gcgccggtca cgacgcgcgt cgtgcagatc
     4501 gagccggggc cgatgccgac cttgacggcg tcggcgccga gccgcatgag gtcgcgcgcg
     4561 ccgctctcgg tcgccacgtt cccggcgatg atcggcgtcg aggcgaatcg cttgcgaagc
     4621 agcttcaacg catcgagaac gttctggctg tgcccgtgcg aggaatcgag tacgagcacg
     4681 tccacctgcg cgcgcacgag ctcgctcgcg cgatcgagca ggtcgcgcga gacgccgacc
     4741 gcggcgccga cgcggagccg cccgagccgg tctttcgcgg cgtcggggta cttaatcgcc
     4801 ttctggatgt ccttgacggt gatcagaccc ttcaggttcc cgtgacggtc gacgacgagg
     4861 agcttctcga tccgatgctt gtgcaggagt tccttcgcct gctcgagcgt cgttccgacc
     4921 ggagtcgtga ccagccgctc ctttgtcatg cgcgacgaaa tcggccgatc gagatgcgtt
     4981 tcgaatctca ggtcccgatt cgtgagaatg ccgacgagct tccccttctc gtcgatgacg
     5041 gggacgcccg agatcttgta gcgcgccatg agctcgagcg cgtgcgcgat cttctgctcc
     5101 ggacggacgg ttaccggatc cacgatcatt cccgactccg accgcttgac ccggtccact
     5161 tcctccgcct gcgcagggat gggaaggttc ttgtgaatga cgccgaggcc gccctgctgc
     5221 gccatcgcga tcgcgaggcg ggactccgtc accgtgtcca ttgcggcgga gatgatcgga
     5281 atgttcaggg cgaccgtgtc tgtcagccgc gtcttcaggt ccacgtcccg cgggtgaacg
     5341 cgggaacggg cgggcagaag cagaacgtcg tcgaaggtga gggcgagcgg aaggccgtcg
     5401 gaggagcgtc cggagggccg tcggcgcgcc ggcatcaggc ccccgccgca gccgcgccgt
     5461 ggcacttctt gtatttcttt ccggagccgc atgggcaggg gtcgttgcgt ccgactttgg
     5521 gcccggctcg gtggatcgtc tcgaccttcg cgtccgaacc gcccgacgcg gccgcgccgt
     5581 aagagtagtt gacgttcggc gagcgccgcg acggcatggg caccggcgcg tgccgggcct
     5641 cctcgtcgac gagctctccc tcggcttcgc ggatgaccgg ctggagccgg aagaggtact
     5701 ggacgatctg gtcctcgatc cgctccttca tgagagtgaa gagctcgaac gactccttct
     5761 tgtactcgac gagcggatcg cgctggccgt agccgcgcag cccgatgccc tccttcaggt
     5821 ggtcgatcgc gaggaggtgg tccttccact gctggtccac gacctggagc atcacgaacc
     5881 gctcgtggag gcgcatggtc tcggggccga gcagcttttc cttcgattcg tagatctcgt
     5941 ggatcgcaga gaggagcgtc tcgcggatct cgtcgcgttc cttgcccgag aagtcgatcg
     6001 agccgggggc aatcccgaag tactccttca gatcgaggtc gaacgccggc aggtcccact
     6061 cgtcgggggc cttgtcctcc ggcaggctcc gtccgatcgc gtagtcgagg atgacctcgg
     6121 cgagtcccag gatctcgtcc ttgctctgct tgcccttcag gatgtcgcgg cgaagcccgt
     6181 agatctcctc gcgctgccgg ttcatgacgt cgtcgtactc gagcaggtgc ttgcgggtct
     6241 cgaagttcct tccctcgacc tgcttctgcg ccttttcgat cgcgcgcgac accatcccgt
     6301 gttcgatcgg gaccccttct tccatgccga gctttcgcat gacgttctgg agccgatccg
     6361 atccgaagat ccgcatgaga tcgtcttcga gcgacaggta gaagcgcgac gagcccggat
     6421 ctccctgccg gccgctgcgg ccgcggagct ggttgtcaac ccggcgcgac tcgtgtcgtt
     6481 cggtgccgag gatgtggagg ccgcccgctg cgagcacggt ctcccggtcc cgggccgcct
     6541 ccgctcgaat cgtggcgagc atcgtcgtga actcgtcggg cgacttcg
//
LOCUS       DASWAV010000648         4156 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U2665
            k141_355798, whole genome shotgun sequence.
ACCESSION   DASWAV010000648 DASWAV010000000
VERSION     DASWAV010000648.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960316
            Sequence Read Archive: SRR10260007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 4156)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4156)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:47:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,929
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4156
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_355798"
                     /isolate="SMAG_U2665"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.36444 N 132.31889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..189)
                     /locus_tag="VF958_13570"
     CDS             complement(<1..189)
                     /locus_tag="VF958_13570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9150183.1"
                     /translation="MPWNRQTWASLSPTGLRETKPGHFREMARIAWDNRRELPFAWRI
                     LTRGVCDGCSLGTTGVRDW"
     gene            complement(227..682)
                     /locus_tag="VF958_13575"
     CDS             complement(227..682)
                     /locus_tag="VF958_13575"
                     /inference="COORDINATES: protein motif:HMM:NF025170.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PCYCGC motif-containing (lipo)protein"
                     /protein_id="HEX9150184.1"
                     /translation="MRARFLLVVPIVALAVIAAAPSTKTAPAKASAQNDCATCTERGV
                     VLDPARFSDTRIYEREVKPAYEIARKYPKTLDALHCFCECQESMVHRHKTLLTCFTSQ
                     HAAGCGICMREAMLAGELKDKGLPDDQIENTVESAFKTDGHRPTQGHPG"
     gene            complement(750..1793)
                     /locus_tag="VF958_13580"
     CDS             complement(750..1793)
                     /locus_tag="VF958_13580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011521406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:ferredoxin oxidoreductase subunit
                     beta"
                     /protein_id="HEX9150185.1"
                     /translation="MSSVAEPKAAPTNRRGLTVKEYGGLKSTLCVGCGHDVITRQITQ
                     AFYEMGVDPHKVAKMSGIGCSSKTTAYFVDKGHGFNAVHGRMPAVATGAMLANPHLLT
                     IGVSGDGDTASIGAGQFVHLLRRNVPMIYIVENNGVYGLTKGQFSATADLGSKLKTGV
                     VNDLPAMDLCALAIELGCGYVARSFSGDMKQLLALIKGAIAHRGTALLDVISPCVTFN
                     NHEDSTKSYKWAKDHEIAIQEIGFVPYFEEITVDYDPGTTQDVVLYDGSHIRLRKLRE
                     DYDATDKMKALALLNEGTEKQEFLTGLIYVAPQNKDFLSLLDLPDRPLSIVADDKARP
                     PREALGQMMEELK"
     gene            complement(1793..3646)
                     /locus_tag="VF958_13585"
     CDS             complement(1793..3646)
                     /locus_tag="VF958_13585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011521405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase subunit alpha"
                     /protein_id="HEX9150186.1"
                     /translation="MSTAILEEKAPAPSTNGPAVVNDFSIHVATANGSGSQSSNNILM
                     RSIFQMGIPVSGKNLFPSNIQGLPTWFTIRASKEGFIARRRDIGVLICMNPETAHEDV
                     RTATSGAAVIYEESLKLDALRNDVHFYPVPFSKLVLEFVHDGKLRRLAVNMIYVGVVA
                     WLLGIDMAEIERAVAKQFKNKAKAVEINLGAIRGGWDWASKNLTKVDPYRLERMNATS
                     GKIIIDGNAACAIGCVMGGVSVVTWYPITPSSSLAESLTDYARRFRMNPEDGKPTFAI
                     VQAEDELASIGMAIGAGWAGARSMTATSGPGISLMAEFTGLAYYAEVPVVIFDVQRVG
                     PSTGLPTRTMQGDILTTYNLSHGDAQHILLIPGSVQECYEMAIEAFNLAERFQTPVFV
                     MTDLDLGMNNWMSDPFPYPEKPFDRGKVLRTNEEIEEHVKRFGEFARYKDVDGDGIPW
                     RTLPGITDHPLGTYFTRGSGHSERATYTERPDDYKNNMDRLARKHETARKWVPRPIVS
                     NGTGSRVGLIAYGSSDFAVEEARVILKKRGVETDYLRIRALPFSADVAEFVARHDRVY
                     VVDQNRDRQMYEIIAVEVGGDSGKLRSIRHYDGFSLDAESVVEGIEAGEKR"
     gene            3730..>4156
                     /locus_tag="VF958_13590"
     CDS             3730..>4156
                     /locus_tag="VF958_13590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HEX9150187.1"
                     /translation="MKSIGIRAISAAIFLVLAAHADGAGRPFRFEELAKAERIGGFDL
                     SPDGKWIAYAVGTTVVAENRTKSAIWLAPVAGGAPRRLTSGEKRDSDPQFSPDGRRVA
                     FLSNRDDSSQIWTVDLSGGEPSKATTFPTEVSGFQWSPDG"
BASE COUNT          664 a         1326 c         1389 g          777 t
ORIGIN      
        1 ccagtcccgg acacccgtcg tcccgagcga gcatccgtcg cagaccccgc gcgtgaggat
       61 cctccaggcg aacggcagct cccgccggtt gtcccacgcg atccgggcca tctcgcggaa
      121 gtggcccggc ttggtctcac gaaggccggt gggggagagc gaggcccacg tctggcgatt
      181 ccacggcacg ccccgatctt atcgaggcgt cctgccgggt cgagatctat cccgggtgcc
      241 cctgcgtcgg ccggtggccg tcggtcttga aggccgattc gacggtgttc tcgatctggt
      301 cgtccgggag gcccttgtcc ttcagctctc cggcgagcat cgcttcgcgc atgcagatcc
      361 cgcagcccgc cgcgtgctgg ctcgtgaagc acgtcaggag ggtcttgtgg cgatggacca
      421 tgctctcctg gcactcgcag aagcagtgga gggcatcgag cgtcttcggg tactttcgcg
      481 cgatctcgta agccggcttg acctcgcgct cgtagatccg ggtgtcggag aagcgggccg
      541 ggtcgagaac gactccgcgc tcggtgcagg tcgcgcaatc gttctgcgcg gacgcctttg
      601 ccggggccgt cttcgtcgac ggagcggcgg cgatgacggc gagggcgaca atcggaacga
      661 cgagaagaaa tcgagctcgc attcggacct cccgggccgc taacgccgag gagggaatct
      721 ttcttccctc gagtgcccga gggagctcgc tatttcagct cctccatcat ctgcccgagg
      781 gcctcgcggg gcggacgcgc cttgtcgtcg gccacgatcg acaggggacg gtcgggcagg
      841 tccaagagcg agaggaagtc cttgttctgg ggcgccacgt agatcaggcc cgtcaggaac
      901 tcctgcttct cggtgccttc gttcaggaga gcgagcgcct tcatcttgtc ggtggcgtcg
      961 tagtcctcgc gcagtttcct caaccggatg tgggagccgt cgtaaaggac cacgtcctgc
     1021 gtcgtaccgg ggtcgtagtc gacggtgatc tcctcgaagt acggaacgaa cccgatctcc
     1081 tggatcgcga tctcgtggtc cttcgcccac ttgtacgact tcgtcgagtc ctcgtggttg
     1141 ttgaacgtga cgcacggcga gatgacgtcg aggagagccg tcccccgatg ggcgatcgcg
     1201 cccttgatca gcgcgaggag ctgcttcatg tctccggaga acgaacgagc gacgtacccg
     1261 cagccgagct cgatcgcgag cgcgcacagg tccatcgcgg gaaggtcgtt gacgacgccg
     1321 gtcttcagct tcgatccgag gtccgcagtc gcggagaact gtcccttcgt caggccgtag
     1381 acgccgttgt tctcgacgat gtagatcatc ggcacgttgc ggcggaggag gtgaacgaac
     1441 tgcccggctc cgatcgaggc cgtgtcgccg tctcccgaga cgccgatcgt cagaaggtgc
     1501 gggttggcga gcatggctcc cgtcgcgacc gccggcatgc gtccgtggac ggcgttgaag
     1561 ccgtggccct tgtcgacgaa atacgcggtc gtcttcgagg agcagccgat gcccgacatc
     1621 ttcgcgacct tgtgggggtc gacccccatc tcgtagaagg cctgcgtgat ctgccgggtg
     1681 atgacgtcgt gcccgcagcc gacgcacagg gtcgacttca ggccgccgta ctccttgacg
     1741 gtcaggcccc ggcgattggt cggggcggct ttcggttcgg cgacggagga catcagcgct
     1801 tctccccggc ctcgatgccc tcgacgacgc tctcggcgtc gagggagaag ccgtcgtaat
     1861 ggcggatcga tcgaagcttg ccgctgtcac cgccgacctc gacggcgatg atctcgtaca
     1921 tctgccggtc gcggttctga tcaacgacgt agacgcggtc gtggcgcgcg acgaactcgg
     1981 ccacgtccgc gctgaacggc agcgcgcgga ttctcaggta gtcggtctcg actccgcgct
     2041 tcttgagaat gacgcgagct tcctcgacgg cgaaatccga cgagccgtaa gcgatcaggc
     2101 cgacgcgaga acccgtgccg ttcgagacga tcgggcgcgg gacccacttc ctcgccgtct
     2161 cgtgcttgcg cgcgagccgg tccatgttgt tcttgtaatc gtcgggccgc tcggtgtacg
     2221 tggcgcgctc ggaatggccg gacccgcggg tgaagtaggt gccgagcgga tggtcggtga
     2281 tccccggcag agtgcgccag gggatgccgt cgccatcgac atccttgtag cgggcgaatt
     2341 cgccgaagcg cttgacgtgc tcctcgatct cctcgttcgt gcggagcacc ttgccccggt
     2401 cgaacggctt ttccgggtaa gggaacggat cgctcatcca gttgttcatc ccgaggtcga
     2461 gatcggtcat gacgaacacg ggcgtctgga accgctcggc gagattgaac gcctcgatcg
     2521 ccatttcgta gcactcctgg acgctcccgg ggatgagcag gatgtgctgc gcgtcgccgt
     2581 gcgaaaggtt gtaggtcgtg aggatgtcgc cctgcatcgt ccgcgtcggg agccccgtgg
     2641 aggggccgac gcgctgcacg tcgaaaatga cgaccgggac ctcggcgtag tacgcgaggc
     2701 cggtgaactc ggccatgagc gagatgccgg gtccggaggt cgccgtcatg gatcgcgccc
     2761 ccgcccatcc ggcgccgatc gccattccga tcgacgcgag ctcgtcttcc gcctgcacga
     2821 tcgcgaacgt gggcttcccg tcctcgggat tcatccggaa ccgccgcgcg tagtcggtca
     2881 gcgattcggc gagcgacgag gacggcgtga tcggatacca cgtcacgacc gagactccgc
     2941 ccatgacgca cccgatggcg cacgccgcgt tcccgtcgat gatgatcttg cccgacgtcg
     3001 cgttcatccg ctcgagacgg tagggatcca ccttcgtcag gttcttcgag gcccagtccc
     3061 acccgccgcg aatcgctccg aggttgattt cgaccgcctt ggccttgttt ttgaactgct
     3121 tggcgacggc gcgttcgatc tccgccatgt cgatcccgag gagccacgcg accaccccga
     3181 cgtagatcat gttgaccgcc aggcggcgga gcttgccatc gtggacgaac tccaggacga
     3241 gcttcgagaa ggggaccgga tagaaatgga cgtcgttgcg gagcgcgtcg agcttgaggg
     3301 actcctcgta gatcaccgcc gctccggagg tggccgtccg gacgtcttcg tgtgccgtct
     3361 ccgggttcat gcagatgagc acgccgatgt ctcgccgccg cgcgatgaag ccttccttcg
     3421 aggcgcggat cgtgaaccag gtcggcagcc cctggatgtt tgacgggaac aggttctttc
     3481 ccgaaacggg tattcccatc tggaaaatcg accgcatgag gatgttgttc gaggactggc
     3541 tgcccgatcc gttggcggtc gcgacgtgaa tcgagaaatc gttgaccacc gccggaccat
     3601 tcgtcgaggg tgcgggggcc ttctcttcca gaatcgccgt ggacatgtgt ctcctttgcc
     3661 tgcggttgcg ttgcaccggg gccgaaaatc atagcagagc aagaacatag aatcctcggg
     3721 ggaggcagca tgaaatcgat tgggatccga gcgatttccg ccgcgatctt cctcgtcctg
     3781 gccgcgcacg cggatggggc cgggcggccg ttccggttcg aagagctggc gaaggccgag
     3841 cggatcggcg gcttcgatct ctctcccgac ggcaagtgga tcgcctacgc cgtgggcacg
     3901 acggtcgtcg ccgagaaccg cacgaagtcg gcgatctggc tggcgcccgt cgccggcggc
     3961 gcgccgcggc ggttgacctc cggggagaag cgcgactccg acccgcagtt ctcgcccgac
     4021 ggccgccggg tcgcctttct ctcgaaccgc gacgattcct cgcagatctg gacggtcgat
     4081 ctctccggcg gcgagccatc gaaggcaact acctttccga cggaggtcag cggtttccag
     4141 tggtcgcccg acggca
//
LOCUS       DASWAV010000649         2238 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U2665
            k141_478685, whole genome shotgun sequence.
ACCESSION   DASWAV010000649 DASWAV010000000
VERSION     DASWAV010000649.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960316
            Sequence Read Archive: SRR10260007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2238)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2238)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:47:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,929
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2238
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_478685"
                     /isolate="SMAG_U2665"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.36444 N 132.31889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..796
                     /gene="ubiE"
                     /locus_tag="VF958_13595"
     CDS             <1..796
                     /gene="ubiE"
                     /locus_tag="VF958_13595"
                     /EC_number="2.1.1.163"
                     /EC_number="2.1.1.201"
                     /inference="COORDINATES: protein motif:HMM:NF001244.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052624 - 2-phytyl-1,4-naphthoquinone
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0042372 - phylloquinone biosynthetic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="bifunctional demethylmenaquinone
                     methyltransferase/2-methoxy-6-polyprenyl-1,4-benzoquinol
                     methylase UbiE"
                     /protein_id="HEX9150188.1"
                     /translation="DLTLDVASGDLTLDVASGDLTPLMNKYLSYDEERAPKVREMFSR
                     LAERYNLVNDVMSFGMHRRWKRETVELALEGRRNSARLLDLCCGTGDMCFDAGQLGAG
                     RVVGADFTLPMLGVAKRRARSEAGRPDFIAADALCLPFRDASFDAITVGYGLRNIADP
                     RLALAEMRRVLAPGGRAVVLDFGKPDNAVAAALYNGYLRTMMPAVGWIFHRDPQTYSY
                     IPDSLERFPAQRGVENLMREVGFANVRYENRLLGTMGLNIGEAPIR"
     gene            811..>2238
                     /gene="ettA"
                     /locus_tag="VF958_13600"
     CDS             811..>2238
                     /gene="ettA"
                     /locus_tag="VF958_13600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002623929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0045900 - negative regulation of
                     translational elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-dependent translational throttle protein
                     EttA"
                     /protein_id="HEX9150189.1"
                     /translation="MAHQYIFTMVNLRKVVPPQREILRGIWLSFLPGAKIGVLGPNGA
                     GKSTLLRIMAGVDKDFAGDAFPAEGTRIGYLSQDPRLDPSKDVFGNVEEGVAPTRALL
                     ARFDELNEKLASDLSEADCERMMAEYQRVQDAIEASNAWELDRQLEIAMDALRLPPAD
                     ADVTKISGGERRRVALCKVLLERPDLLLLDEPTNHLDAESVAWLERHLAEFPGTVVAV
                     THDRYFLDNVAGWILELDRGAGIPWEGNYSSWLAQKESRLAVEEKQESARRRSLQREL
                     EWVQMAPRARHAKSRARIERYESLLAEGGPESRIETREIYIPPGPRLGDVVVRADGVR
                     KAYGDRLLMEDLSFDLPRGGIVGVIGPNGAGKTTLFKMIVGLEKPDAGRLTVGETVVV
                     SYVDQDRDRPNPSKTAFEEISGGADKIVLGKREIPARAYAAMYNFKGPDQQKRVGDLS
                     GGEMNRLHLARTLKRGGNLLLLDEPT"
BASE COUNT          385 a          739 c          770 g          344 t
ORIGIN      
        1 agacctgacc ctcgatgtgg caagtggaga cctgaccctc gatgtggcaa gtggagacct
       61 gaccccgtta atgaacaagt acctgtcgta cgacgaagag cgcgcgccga aggtgcggga
      121 gatgttcagc cggctcgccg agcgctacaa cctcgtcaac gacgtcatga gcttcgggat
      181 gcatcgcagg tggaagcgcg agacggtcga gctcgccctc gaagggcgcc ggaactccgc
      241 gcgcctgctc gacctctgct gcgggaccgg cgacatgtgc ttcgacgcgg ggcagctcgg
      301 cgccggtcgg gtcgtcggcg cggatttcac tctgccgatg ctcggggtcg cgaagcgccg
      361 cgcccgctcg gaagccggac gtcccgactt catcgcggcg gacgcgctgt gtctcccctt
      421 ccgtgacgct tccttcgacg cgatcaccgt cgggtacggc ctgcgcaaca tcgccgaccc
      481 gcggctcgcg ctcgcagaaa tgcgccgcgt gctcgcgccg ggaggacgcg cggtcgtcct
      541 cgacttcggg aagccggaca acgcagtcgc ggccgcgctc tacaacggct acctgcggac
      601 gatgatgccg gcggtcggat ggatcttcca tcgcgacccg cagacctaca gctacattcc
      661 ggactctctc gagcgctttc ccgcgcagcg cggggtcgag aacctgatgc gggaagtcgg
      721 cttcgcgaac gtgcgctacg agaaccggct tctcggaacg atgggattga acatcggaga
      781 agctccgatc cgatagactc ccccgttctc atggcgcacc agtacatctt cacgatggtc
      841 aacctgcgga aagtcgtacc tccgcagcgg gagatcctcc gcgggatctg gctctcgttt
      901 ctccccggcg cgaagatcgg tgttctcggg ccgaacggcg cgggcaagtc gacgctcctg
      961 cggatcatgg cgggcgtcga caaggacttc gccggtgacg cgtttccggc cgaggggacg
     1021 cggatcggct acctgtcgca ggacccgcgc ctcgatccgt cgaaggacgt cttcggcaac
     1081 gtggaagagg gggtcgcgcc gacgcgcgcc ctcctcgccc ggttcgacga gctgaacgag
     1141 aagctcgcga gcgacctctc cgaagcggac tgcgaacgca tgatggccga gtatcagcgc
     1201 gtccaggacg cgatcgaggc gtcgaacgcg tgggagctcg accgacagct cgagattgcg
     1261 atggacgccc tgcggctgcc gccggcggac gccgacgtga cgaagatctc cggcggcgag
     1321 cgacggcgcg tcgcgctctg caaggtgctc ctcgagcggc cggacctcct cctgctggac
     1381 gagccgacga accatctcga cgcggagtcc gtcgcctggc tcgagcgcca cctcgcggag
     1441 tttcccggta cggtcgtcgc ggtcacgcac gatcgctact tcctcgacaa cgtcgcggga
     1501 tggatcctcg aactcgaccg cggggcggga atcccgtggg agggcaacta ttcgagctgg
     1561 ctcgcccaga aggagtcgcg gctcgccgtg gaggaaaagc aggagtccgc gcgccgtcgc
     1621 tcgctgcagc gcgagctcga gtgggtgcag atggcgccgc gcgcgcggca cgcgaagagc
     1681 cgggcgcgca tcgaacggta cgagtcgctg ctcgccgagg gcggaccgga gtcgcgcatc
     1741 gagacgcgcg agatctacat tccgcccggg ccgcgcctgg gagacgtcgt cgtgcgggcc
     1801 gacggcgttc gcaaggcgta cggcgaccgg ctgctgatgg aggacctttc gttcgacctc
     1861 ccgcgcggcg gaatcgtcgg agtcatcgga cccaacggtg ccggcaagac gacgcttttc
     1921 aagatgatcg tggggctcga aaaaccggac gccggccgtc tcacggtcgg cgagacggtc
     1981 gtcgtgtcct acgtcgacca ggatcgcgac cggccgaatc cctcgaagac cgcgttcgag
     2041 gagatctcgg gcggcgcgga caagatcgtt ctcggaaagc gcgagatccc cgcgcgcgca
     2101 tacgccgcga tgtacaactt caagggaccg gaccagcaga agcgtgtggg agatctctcc
     2161 ggcggcgaga tgaaccggtt gcacctcgcg cgcacgctga agcgcggagg aaacctgctc
     2221 ctcttggacg agccgacc
//
LOCUS       DASWAV010000650         3539 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U2665
            k141_519680, whole genome shotgun sequence.
ACCESSION   DASWAV010000650 DASWAV010000000
VERSION     DASWAV010000650.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960316
            Sequence Read Archive: SRR10260007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 3539)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3539)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:47:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,929
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3539
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_519680"
                     /isolate="SMAG_U2665"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.36444 N 132.31889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(27..302)
                     /locus_tag="VF958_13605"
     CDS             complement(27..302)
                     /locus_tag="VF958_13605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012629519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF167 domain-containing protein"
                     /protein_id="HEX9150190.1"
                     /translation="MTPLPPFARRVGGGIELRIKVVPGASRSEIVGILGNRLKVRVAA
                     PPEAGKANRAVIELLRGWLGVREVEIASGVSSPEKTVLVLGLSRLPS"
     gene            complement(299..634)
                     /locus_tag="VF958_13610"
     CDS             complement(299..634)
                     /locus_tag="VF958_13610"
                     /inference="COORDINATES: protein motif:HMM:NF017103.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DciA family protein"
                     /protein_id="HEX9150191.1"
                     /translation="MRRARGGFSSLEAAADGVVRRMSAGEGALLWIQARWPRIVGEPL
                     SRKIVPLELSGYRLTLSLLDPAWKKPIEAAIPELERRLAVELPRVRPRVFLRETGDRR
                     RETTRNPRI"
     gene            complement(631..1671)
                     /locus_tag="VF958_13615"
     CDS             complement(631..1671)
                     /locus_tag="VF958_13615"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012282307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008465 - glycerate dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycerate dehydrogenase"
                     /protein_id="HEX9150192.1"
                     /translation="MATRSDEESDVAAAHEPEPAQEVRSAAGVILATRALPEPSLSTL
                     QPDYQVRVLGYPPNEHELAAEVADVDALITLVSDPVTEAVIRAGRKLRIVANYAVGVN
                     NIDRAAAAARGIVVTNTPGVLTDATADLTLALILALARRVVEGDRMVRAGGFAGWAPD
                     LLLGRELKGKVLGIVGPGRIGRAVARRARAFGMTVIAFARSARADDDPDDPPRVSFDD
                     LLRRADVVSLHVPLTDDTRHLFNRETFLKMKPGSLLINTARGGLIDETALIAALESGQ
                     IGGAGLDVYENEPAILPGLLDDDRVVLMPHAGSATTEARREMARMVVEDVRRVLGGER
                     PLRAVSPDPVKR"
     gene            complement(1724..1885)
                     /locus_tag="VF958_13620"
     CDS             complement(1724..1885)
                     /locus_tag="VF958_13620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9150193.1"
                     /translation="MHLAARSLQFAGLVVTGIAFFDGVLAGNVRRELVLLALGAGLFF
                     LGRALQRNR"
     gene            complement(1892..>3539)
                     /locus_tag="VF958_13625"
     CDS             complement(1892..>3539)
                     /locus_tag="VF958_13625"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF012387.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HEX9150194.1"
                     /translation="ETSVAQAAARAIGFLGRGDGQDALTAAVETAASPEPRATILRSL
                     WRFAAPATAQVALRFANDPDARVRFGAIYALARKPLESSLPALTAALSDSDADIAASA
                     ARALGVLAKKESAEPLAAALDSGKAPLVANALVALEAVLEKNPGTSVGKGRIARVLSL
                     AGDANPNVAVPALTLLRQFAGADRDVFRRLWTIATTGAGYRRQVALSSATAVLRDNAL
                     PALENAAKSSEVPLRAAAAEALGYLTFAKASALRSKLAADPAPLVRGAILASLRTAEA
                     VSQNRALVQAAANDPDPGVRAAAIDALTRLSDASVIPLLSEAISKAAAVRDPDVPLAV
                     IAAAEKLRAEPGAQALVETAYRHSNPVVARIARRSLLQNFRVNAATFPPPEFRPSRTP
                     ADYLALVAESKKPWMASVETARGAFRIRLAGAAAPITVMNFVSLAQKGYFNGSTFHRV
                     VPNFVIQDGDPTGTGNGGPGYEIRDELNPIEYSEGTVGMALSGPDTGGSQWFATQAPQ
                     PHLDGIYTVFGQVVAGQDVVERIEQGDRITRVSVAEAPAP"
BASE COUNT          552 a         1217 c         1256 g          514 t
ORIGIN      
        1 gacgatcccc tcctgctctc gctcgatcag ctcggaagcc gcgacagccc gaggacaagg
       61 accgtcttct ccgggctcga cactccggag gcgatttcga cctctcgcac gccgagccat
      121 ccccgcagga gctcgatgac cgcccggttc gcctttccgg cctcgggcgg cgcggcgacg
      181 cggaccttca accggttgcc gaggatgccg acaatctccg aacgcgacgc gcccggcacg
      241 accttgatcc tgagctcgat cccgcctcca actctccgcg cgaacggagg cagtggagtc
      301 agatccgagg atttctcgtc gtctcccgtc ttctgtctcc cgtctctcgc aagaacacgc
      361 gcggccgaac gcgaggcagc tcgacggcga gccgccgctc gagctccggg atcgcggcct
      421 cgatcggttt tttccacgcg gggtcgagca gagacagcgt gaggcggtaa cccgacagct
      481 cgagcggaac gatcttccgg gacaacggct cccccacgat ccgcggccag cgcgcctgga
      541 tccagaggag agccccctca ccggccgaca tccggcgtac gacgccgtcg gccgctgcct
      601 cgagagagct gaagccaccg cgcgcgcgtc tcatcgcttg accggatcgg gagagaccgc
      661 tcgcagcggt ctctcgccgc cgaggacgcg ccgcacgtcc tcgacgacca tgcgcgccat
      721 ctcgcgccgc gcctccgtcg tcgcgctgcc ggcgtgcggc atcaagacga cgcgatcgtc
      781 gtccaggagt ccgggaagga ttgccggctc gttctcgtag acgtcgaggc ccgccccgcc
      841 gatctgtccc gactcgagcg ccgcgatgag agcggtctcg tcgatgagtc ctccgcgcgc
      901 cgtattgatg agcaggctcc ccggcttcat cttgaggaaa gtttcgcggt tgaacagatg
      961 gcgggtgtcg tcggtcagag ggacatggag cgagacgacg tcggcccgcc ggagaaggtc
     1021 gtcgaacgag acccgcgggg ggtcgtccgg gtcgtcgtcg gcacgcgccg agcgggcaaa
     1081 agcaatcacc gtcattccga aggcgcgggc gcgtcgagcg accgctcttc cgatccggcc
     1141 ggggccgacg atcccgagca ccttgccttt gagctcccgg ccgaggagca gatccggcgc
     1201 ccacccggcg aacccgcctg ctcgaaccat tcggtcccct tcgacgacac gccgcgcgag
     1261 ggcgaggatg agcgcgagcg tcaggtccgc ggtcgcgtcg gtgaggacgc cgggcgtgtt
     1321 cgtcaccacg atcccgcggg cggcagcggc tgcgcggtcg atgttgttca cgccgaccgc
     1381 gtagttcgcg acgatcctga gctttcggcc ggcccggatc acggcttccg tgacgggatc
     1441 gctgacgaga gtgatcagcg cgtcgacgtc ggcgacctcg gccgcaagct cgtgctcgtt
     1501 cggagggtac ccgagaacgc gcacctggta gtcgggctgg agcgtcgaga gggacggttc
     1561 gggcagggcg cgcgtcgcca ggatgacgcc ggcggcagag cgcacctcct gtgcgggttc
     1621 gggttcgtgc gcggccgcga cgtcggactc ttcatcggag cgggtcgcca aggttccccc
     1681 cgtgaaactg ccgtgttgga agcggcgagt ctagcacccg ggttcagcgg ttgcgctgca
     1741 gtgcgcgtcc gagaaagaag agcccggcgc cgagcgcgag caggacgagc tcgcggcgga
     1801 cgtttcccgc gagaacaccg tcgaagaacg cgataccggt aacgaccagt ccggcgaact
     1861 ggagcgatcg tgcggcgaga tgcaacgcga cctacggcgc cggcgcctcg gcgaccgaga
     1921 cccgcgtgat tcgatctccc tgctcgattc tctcgacgac gtcctgaccg gccacgacct
     1981 gaccgaaaac cgtatagatc ccgtcgaggt ggggttgcgg cgcctgcgtc gcgaaccact
     2041 ggctcccccc ggtgtccgga cccgagagcg ccatcccgac ggttccctcg gagtattcga
     2101 tcgggttcag ctcgtcgcgg atctcgtagc ccgggcctcc gttaccggtc ccggtcggat
     2161 ccccgtcctg gatcacgaaa ttcggcacga cacggtggaa agtcgagccg ttgaagtacc
     2221 ccttctgcgc gagagacacg aagttcatga cggtgatcgg cgcggccgca ccggcgagcc
     2281 ggatccggaa ggcgccgcgg gccgtttcga cggaggccat ccacggtttc ttcgactcgg
     2341 cgacgagcgc gaggtagtcg gcaggggtgc gagacggccg gaactccggt ggtggaaagg
     2401 ttgccgcgtt cacgcggaag ttctgcagga gcgaccggcg ggcaatccgc gccacgacgg
     2461 ggttcgaatg ccggtatgcc gtttccacga gagcctgggc tcccggctcg gcgcggagct
     2521 tttccgcggc ggcgatcacc gcgagcggga cgtcgggatc gcggaccgcg gccgccttcg
     2581 agatggcctc ggagaggagc ggaatcaccg aggcatccga caggcgtgtc agggcgtcga
     2641 tcgcagcagc gcggacgccg ggatcggggt cgttcgcggc cgcctgcacg agagcgcggt
     2701 tctgcgaaac ggcctccgcc gtccgcaggc tcgcgagtat cgcgcccctc acgagggggg
     2761 ccgggtccgc cgccaacttc gagcggaggg cgctcgcctt ggcgaacgtg aggtagccga
     2821 gagcttccgc ggcggccgcg cgcagcggca cctccgacga tttcgccgcg ttctcgagag
     2881 cgggaagcgc gttgtcgcgg agcaccgcgg tcgccgaaga gagagcgacc tgccgccggt
     2941 aacccgcccc ggtcgtcgcg atcgtccaca gccggcggaa cacgtcacgg tccgcgccgg
     3001 cgaactgccg gagcagcgtg agagcgggga cggcgacatt cgggttcgcg tcgccggcga
     3061 gagagagaac gcgcgcgatc cgccccttcc ccacggaggt tccgggattc ttctcgagaa
     3121 cggcctcgag ggcgacgagc gcgttcgcga cgaggggcgc cttgccgctg tcgagtgcgg
     3181 cggcgagcgg ctccgccgac tctttcttcg cgaggactcc gagggcgcgc gccgcggagg
     3241 cagcgatgtc cgcgtcgctg tcggagaggg ccgccgtcag cgccggcaga gacgactcga
     3301 gcggcttccg cgcaagggcg tagatcgcac cgaaacgcac gcgtgcgtcc ggatcgttcg
     3361 cgaagcgaag ggccacctgc gccgtcgccg gcgcagcaaa gcgccagagc gaccgcagga
     3421 tggtggcgcg cggttccggc gacgccgccg tctcgacggc ggcagtcagg gcgtcctggc
     3481 cgtcgcctcg gccgaggaaa ccgatcgccc gggccgcggc ctgcgcgacc gaggtctcg
//
LOCUS       DASWAV010000651         3313 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U2665
            k141_677560, whole genome shotgun sequence.
ACCESSION   DASWAV010000651 DASWAV010000000
VERSION     DASWAV010000651.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960316
            Sequence Read Archive: SRR10260007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 3313)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3313)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:47:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,929
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3313
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_677560"
                     /isolate="SMAG_U2665"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.36444 N 132.31889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..280
                     /locus_tag="VF958_13630"
     CDS             <1..280
                     /locus_tag="VF958_13630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9150195.1"
                     /translation="GKGVSLVLADRREEAEKILAEFEERLKKQYVPMAAMVQMNIALG
                     HTDDAFRWLEKAADYRASFMMPIKVYPFFDPIRSDPRFTSLLERYGLA"
     gene            complement(277..663)
                     /locus_tag="VF958_13635"
     CDS             complement(277..663)
                     /locus_tag="VF958_13635"
                     /inference="COORDINATES: protein motif:HMM:NF025078.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4157 domain-containing protein"
                     /protein_id="HEX9150196.1"
                     /translation="MARTPLPIPLADRLLPAFEHEILRARLHRGRFARILTSLAGANA
                     IVFGRRVFLGVSAERAIAAGAAAAAALLAHELTHVRQYRRYGMAAFLGRYVGEYLRAR
                     IAGASHPEAYCRISFEREAERASRNC"
     gene            complement(665..1741)
                     /locus_tag="VF958_13640"
     CDS             complement(665..1741)
                     /locus_tag="VF958_13640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9150197.1"
                     /translation="MKLLTCLAGAVVAVTLGAGVARAQTPEPTPTPTAVPPSEVAPSA
                     PAAPEAVPSPAVPEPTPTAVAVPPAVVPTPALPAPTSTAVAVPPAAPTPVAVAPAPIA
                     RVTDAPVVAVLQAPAENPFGVVPEAPAALPPKLTFAEEVAHSAAFVSARIDATGRIMG
                     IRPQRDPIPSLAGESQRSLARWVFDPARKGSESVETWAAMRLDLEVEIDAPRSVQSSL
                     TPVTPTTAVPRPFVWVPDQAWLESRNGAPPDGGVPLETVDNPPMPKKTPWDADSYKGP
                     FSAKFWVKVNSAGVLEKAIPLEASDPILLPYLRRAMSGWAFRPARVNGGSADSWNELH
                     LSGQVTYKVELKQIASLRRSLFGS"
     gene            complement(1779..>3313)
                     /locus_tag="VF958_13645"
     CDS             complement(1779..>3313)
                     /locus_tag="VF958_13645"
                     /inference="COORDINATES: protein motif:HMM:NF012688.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DNA polymerase"
                     /protein_id="HEX9150198.1"
                     /translation="ADAKSLDALLAREGASVEADVFDVCLAQYVLSPGAGSSEFEVMA
                     FQRLGHRVTTDKEAGITSCTLPEGYAVETADRWLGERATGTLALAERLRPEMEKKPEL
                     EKIYREIERPLTPVLARMELAGIRVDIPYLNDMSARMDRDLRALEEKIWAEAGEQFNV
                     NSPVKLGQILFEKMKYPVGRKTAKTRVSSTGVEVLNELAEQGFPIARLVIEYREISKL
                     KGTYVDALPQLADSESRVHSSFHQTVAATGRLSSSDPNLQNIPIRTAAGREIRRAFVA
                     PAGRKLVIADYSQIELRILAHLSNDAALIGAFERGDDIHRATASKIFGVSPDLVSTEM
                     RFAAKRINFALLYGMAAYTLGKDLGVPTAEAKSYIESYFAQFPAVRGTLDGILEEARR
                     TKEVRTIFGRVRPIPDIHASNGMVRGNAERMALNAPFQGSAADIIKIAMVRLDRALTE
                     GRSGARMLLQVHDELVLEAPEKEVEEVSALVRRVMEGSAQLRVHLAVDVGAADNWLDA
                     KK"
BASE COUNT          540 a         1083 c         1157 g          533 t
ORIGIN      
        1 cgggaaaggc gtgagtctcg ttctcgctga ccgtcgcgaa gaagccgaga aaatcctcgc
       61 cgagttcgag gagcggctga agaagcagta cgtgccgatg gccgcgatgg tgcagatgaa
      121 catcgcgctc gggcacaccg atgacgcgtt ccggtggctc gagaaggcgg cggactatcg
      181 ggcgagtttc atgatgccga tcaaggtcta tccattcttc gatccgatcc gctcggatcc
      241 tcggttcact tcgttgctcg agcggtacgg gctcgcctag cagttccggg acgcccgctc
      301 cgcctcccgc tcgaacgaga ttctgcagta cgcctccggg tgcgacgcgc cggcgatccg
      361 ggccctcaga tattccccga cgtaacggcc gaggaaagcg gccattccgt agcgtcggta
      421 ctgacgcacg tgcgtgagct catgcgccag gagggcggcc gcggcggcgg cgccggcggc
      481 gatcgcgcgt tcggcggaca ctccgaggaa gacgcgtcgc ccgaagacga tcgcgttcgc
      541 gccggcgagg gacgtcagga tgcgtgcgaa gcgaccgcgg tgaagtcggg cgcggaggat
      601 ctcgtgctcg aacgcaggga ggaggcggtc ggcgagggga atcggaagcg gagtgcgcgc
      661 cagcctaaga cccgaagagc gatcggcgaa gcgacgcgat ctgcttcagc tccaccttgt
      721 aggtgacctg gcccgagaga tggagctcgt tccaggagtc cgcggacccg ccgttcacgc
      781 gggcgggacg gaaggcccat cccgacatgg ctcggcgcag ataaggaagg agaatcgggt
      841 cgctcgcttc gagcggaatc gccttttcga ggacgccggc ggagttgacc ttgacccaga
      901 acttcgccga gaacgggccc ttgtaggagt ccgcatccca cggcgttttc ttcggcatcg
      961 gcgggttgtc gaccgtctcg agcggaacgc ctccgtccgg cggtgcgccg ttacgactct
     1021 cgagccacgc ctggtcgggg acccacacga aggggcgcgg gaccgcggtc gtcggcgtca
     1081 ccggcgtcag ggagctctgg accgagcgcg gcgcatcgat ctcgacctcg aggtcgaggc
     1141 gcatcgcagc ccatgtctcg acgctctccg agcctttccg cgcgggatcg aacacccacc
     1201 gagcgagcga ccgctgcgac tcgccggcga gcgacgggat cggatctctc tgggggcgga
     1261 ttcccatgat gcggccggtc gcgtcgatgc gagcggacac gaacgccgcg ctgtgcgcaa
     1321 cctcttctgc gaatgtcagt ttcggcggca gagccgccgg agcttccggg acgactccga
     1381 aagggttctc cgcgggcgcc tgaaggacag cgacgaccgg cgcgtcggtc acccgagcga
     1441 tcggagcggg tgcgaccgcg acgggcgttg gagccgccgg cgggacggca actgcagttg
     1501 aagtcggcgc ggggagagcg ggagtgggaa ccaccgccgg cgggacggcg actgcggttg
     1561 gagtcggctc ggggacagcg ggagatggaa cagcctcggg tgcggcgggc gctgaaggcg
     1621 cgacctcgga tggggggacc gccgtcggcg tcggggtcgg ctcgggcgtt tgcgcgcggg
     1681 cgactcccgc cccgagcgtg acggccacga ccgcgcccgc aagacacgtg aggagtttca
     1741 tcgggatgcc gcggacgtat agcgaaacgt gatgcgcgtc acttcttggc atccagccag
     1801 ttgtcggcgg ccccgacgtc cacggcgagg tggacccgga gctgcgccga accctccatg
     1861 acccgccgga cgagcgcgga gacctcctcg acctccttct ccggtgcctc gagaacgagt
     1921 tcatcgtgca cctgcaggag catccgggcg ccggacctcc cctcggtgag cgcacggtcg
     1981 agccggacca tcgcgatctt gatgatgtcc gccgcggacc cctggaacgg cgcgttcagc
     2041 gccatccgct cggcgtttcc ccgcaccatt ccgttcgacg cgtggatgtc ggggatcgga
     2101 cggacgcggc cgaagatcgt cctcacctcc ttcgtgcggc gcgcctcctc gaggattccg
     2161 tcgagcgttc cccgaaccgc cgggaactgc gcgaagtagg actcgatata gctcttcgcc
     2221 tccgccgtcg ggaccccgag gtccttgccg agcgtgtagg ccgccatccc gtaaaggagt
     2281 gcgaagttga ttcgcttggc ggcgaaccgc atctcggtcg agacgagatc gggcgacacg
     2341 ccgaagatct tcgaagccgt cgcgcgatga atgtcgtcgc cgcgctcgaa ggcgccgatg
     2401 agcgccgcgt cattcgagag gtgcgccagg atgcggagct cgatctgcga gtagtccgcg
     2461 atgacgagtt tgcgtcccgc cggcgcgacg aatgcacggc ggatctcccg tcccgccgcg
     2521 gtgcgtatcg ggatgttctg caggttcgga tcggaggagg acagccggcc ggtcgcggcg
     2581 accgtctggt gaaaggagga gtgcacgcgg gactcggagt ctgcgagttg cggcaacgcg
     2641 tcgacgtacg tgcccttcag cttcgagatc tcgcgatatt cgatgacgag tcgcgcgatc
     2701 ggaaaaccct gctccgcgag ctcgttcaaa acctcgacgc ccgtcgagga gacacgcgtc
     2761 ttggcggtct tcctgccgac gggatacttc atcttctcga agaggatctg gccgagcttg
     2821 acgggcgagt tgacgttgaa ctgctcgccc gcttccgccc agatcttttc ttccagcgcg
     2881 cggaggtccc gatccatgcg cgccgacatg tcgttcaggt aggggatgtc gacgcgaatg
     2941 cccgcgagct ccatccgcgc gagcaccggc gtcagcggcc gctcgatctc gcggtagatc
     3001 ttctcgagct ccggcttctt ctccatctcg ggccggagcc tctctgcgag cgccagcgtt
     3061 ccggtcgcgc gctcgccgag ccaccggtcc gcggtctcca ccgcgtagcc ctccggtagc
     3121 gtgcacgagg tgattcccgc ctccttgtcg gtcgtcacgc ggtgaccgag acgctggaac
     3181 gccatgactt cgaactccga gctccccgcg cccggcgaca gcacatactg cgcgaggcag
     3241 acgtcgaaca cgtccgcctc gaccgaagcc ccctcgcgcg cgaggagagc atcgagcgac
     3301 ttggcatcgg ccg
//
LOCUS       DASWAV010000652         2312 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U2665
            k141_757292, whole genome shotgun sequence.
ACCESSION   DASWAV010000652 DASWAV010000000
VERSION     DASWAV010000652.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960316
            Sequence Read Archive: SRR10260007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2312)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2312)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:47:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,929
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2312
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_757292"
                     /isolate="SMAG_U2665"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.36444 N 132.31889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1496
                     /locus_tag="VF958_13650"
     CDS             <1..1496
                     /locus_tag="VF958_13650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9150199.1"
                     /translation="LDSIESRSLDLPPADVVGMSKNGQMAILLNRRHAGSWITVGTLA
                     RSDFGGGAPHEILENVNDADISPDGTQFAVVRELGTRQQLEFPIGRKIFETTGWVSHP
                     RISPDGKRVAFLEHPAYGNDMGHVALADEHAKVTRLTDAWNGAQGLAWGPGAKEIWFT
                     ATSTGTASGGKGLQYTLWAVRPGGKPRSLYAPPIDLVVEDVSENGAVLLNAIVSRSEI
                     GGLLAGDTRDRDLSTWSDEAAAGISEDGSVFAGVEQSAAGVGIDPFVYYRRARESASV
                     RIGSGAATGLSPDGKWVVTASALPGKGTELTLLPTGAGQPRVISLGTVHHTFSIQRPA
                     SVSADGRRLLFPGKERGHLPRLWLVDLDRTGPPKAISPEGVTSGILSADGQTVAVTDD
                     TGRFLLCPASGSACLPAKGPQPADTPEQWEGSGKAIFVWNRKRTWPAEIYRVDLTTGA
                     RLLWKTISPSDPAGVLYGNILLARDGEHYIYRVRRVTGQLFLGEGLK"
     gene            1493..>2312
                     /locus_tag="VF958_13655"
     CDS             1493..>2312
                     /locus_tag="VF958_13655"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011685392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEX9150200.1"
                     /translation="MTLAAGSRLGPYEILSPLGAGGMGEVYRARDTRLERTVAIKVLP
                     AHLSASPESRQRFEREAKTISQLSHPHICALHDVGREGETEYLVMEYLEGETLADRLL
                     KGPLPREQTLRYGMQIADALDKAHRQGIVHRDLKPGNVMLTKSGVKLLDFGLAKAMAP
                     ATPQSSLTALPTQHALTQEGTILGTFQYMAPEQLEGKEADARTDIFAFGCVLYEMATG
                     KKAFSGATQASLISSILRDDPQPISQVQPMSPPALDRVVQKSLAKDPEDRWQNAA"
BASE COUNT          401 a          727 c          815 g          369 t
ORIGIN      
        1 ggctcgatag catcgaatcg cgctcgctcg atctgcctcc ggccgacgtc gtcggcatgt
       61 cgaagaacgg ccagatggcg atcctgctca accgccggca cgcgggcagc tggatcacgg
      121 tcgggactct cgcgcgctcg gacttcggtg gcggagcccc gcacgagatc ctcgagaacg
      181 tcaacgacgc cgacatcagc ccggacggaa cgcaattcgc ggtcgtccgg gaattgggaa
      241 cgcgccagca gctcgagttc ccgatcggcc ggaagatctt cgagacgacg ggctgggtga
      301 gccatccgag gatttctccc gacggaaaac gggtcgcctt cctcgagcat ccggcgtacg
      361 gcaacgacat gggacacgtc gcgctcgcgg atgaacacgc aaaggtcacc cggctgaccg
      421 acgcctggaa cggggcgcag ggcctcgcct ggggtcccgg cgcgaaagag atctggttca
      481 cggcgacgtc gaccgggacg gcgagcggag gcaagggcct ccaatacacg ctctgggcag
      541 tgcggcccgg ggggaagccg cgctctctct acgcgccacc gatcgatctc gtcgtcgaag
      601 acgtttcgga gaacggagcg gtccttttaa acgccatcgt gagccggagc gagatcggtg
      661 gtcttctcgc gggagacacg cgggaccgtg acctttcgac ctggagcgac gaggccgccg
      721 ccgggatctc ggaagacggg agcgttttcg ccggcgtcga gcagagcgcg gcaggtgtcg
      781 ggatcgatcc tttcgtctac taccgtcggg cccgagaatc ggcttcggtg cgcatcggca
      841 gcggcgcggc gacggggctg tcgcccgacg gaaagtgggt cgtcacggcc tcggctcttc
      901 cggggaaagg gaccgagctc actcttctgc cgaccggagc gggccagccg cgtgtcatct
      961 cactcggaac ggtgcatcac acgttcagta tccagcggcc cgcatccgtg tcggcggacg
     1021 gacgacgact tctctttccg ggcaaggagc gcggacatct tccgcgcctc tggctcgtcg
     1081 atctcgatcg cacgggtcct ccaaaggcga tttctccgga gggagtgacc agcggaattc
     1141 tctcggcgga cggacagacc gtcgccgtta ccgatgacac cggccgattc cttctctgtc
     1201 cagcctccgg aagcgcctgt ttgccggcga agggcccgca gcccgcagat acgccggagc
     1261 agtgggaggg gtcggggaag gcaatcttcg tgtggaatcg gaagcggacc tggccggccg
     1321 agatctaccg ggtcgatctt acgacgggcg cgcggcttct ctggaagacg atttctccat
     1381 cggaccccgc gggtgtgctc tacggaaaca tcctcctggc gcgcgatgga gagcactaca
     1441 tctaccgcgt gcggcgggta accgggcagc tctttctcgg ggaaggtttg aagtgactct
     1501 tgccgcgggc tcccggctcg gtccctacga gatcctgtcg cctctgggcg cgggagggat
     1561 gggggaggtg taccgggccc gggacacgcg gctcgagcgc acggtggcga tcaaggtgct
     1621 cccggcgcat ctctcggctt cgcccgagtc gcggcagcgt ttcgagcggg aggcgaagac
     1681 gatctctcag ctttcgcacc cgcacatctg cgcgctgcac gacgtggggc gcgaggggga
     1741 gaccgagtac ctggtcatgg agtacctgga aggcgagacg ctggcggatc gcctgctgaa
     1801 aggtcctctt ccccgcgagc agacgctgcg gtacgggatg cagattgccg acgcgctgga
     1861 caaggcgcac cggcagggga tcgtgcaccg ggacctgaag ccaggcaacg tgatgttgac
     1921 gaagtcgggc gtgaagctgc tcgacttcgg gctggcgaag gcgatggcgc cggcgactcc
     1981 gcagtcgagc ctgacggcgc ttccgaccca acacgcgctg acgcaggagg ggacgatcct
     2041 gggcaccttc cagtacatgg cgccggagca gctggagggg aaggaagcgg acgctcggac
     2101 ggacatcttc gccttcggct gcgtgctcta cgagatggca acggggaaga aggcgttttc
     2161 gggggcgacg caggcgtcgc tgatttcctc gattctccgg gacgaccccc agccgatctc
     2221 gcaggtgcag ccgatgtcgc caccggcgct cgaccgggtc gtgcagaaga gcctcgcgaa
     2281 agatcccgag gaccgctggc agaacgccgc cg
//
LOCUS       DASWAV010000653         3198 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U2665
            k141_593359, whole genome shotgun sequence.
ACCESSION   DASWAV010000653 DASWAV010000000
VERSION     DASWAV010000653.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960316
            Sequence Read Archive: SRR10260007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 3198)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3198)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:47:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,929
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3198
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_593359"
                     /isolate="SMAG_U2665"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.36444 N 132.31889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1282)
                     /locus_tag="VF958_13660"
     CDS             complement(<1..1282)
                     /locus_tag="VF958_13660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002622142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0035673 - oligopeptide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006857 - oligopeptide transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide transporter, OPT family"
                     /protein_id="HEX9150201.1"
                     /translation="MASVPEKSGAEFQPYVPAGQSMPEFTPKAILLGMFFGILFGAAT
                     VYLALRAGLTVSASVPIAVLAIAVFKKIGKSTILENNIVQTIGSAGESVAAGVVFTVP
                     ALLFLSGGENFFRYLQITILAIFGGTLGVLFMIPLRRALIVKEHGKLPYPEGTACADV
                     LIAGEKGGNLAKMVFAGVGASLLYKFLYGILGLWQETPIFFRTKKESPTYPAATLDVA
                     VTPEYLGLGYIIGPRIASELFSGGLLAWMALIPLIAIFVPETRVVTDLHNLGFSDAWM
                     ANNSYANWIYRAYIRYIGAGAVACAGVMTLIKTLPTIVSAFRESVKSFRGGVEKAEGV
                     KRTDRDLGMGIVVIGSLAIVLILAVLPGFPHGPFPGSLLMSLLIVVFGFFFVTVSSRI
                     VGIIGTSSNPISGMTIATLMATCVVFVVIGWRGDV"
     gene            complement(1364..1579)
                     /locus_tag="VF958_13665"
     CDS             complement(1364..1579)
                     /locus_tag="VF958_13665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_258239534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:complement(1538..1540),aa:Sec)
                     /transl_table=11
                     /product="UXX-star (seleno)protein family 1"
                     /protein_id="HEX9150202.1"
                     /translation="MEFDRVEIFGKDTUPYTTAAREDYARRKIPFTYVDVKRDAKELA
                     RMVELSGGRRVPVIVERGQVTIGFGGT"
     gene            1636..2331
                     /locus_tag="VF958_13670"
     CDS             1636..2331
                     /locus_tag="VF958_13670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HEX9150203.1"
                     /translation="MRTRFFAAAALFSALVPAVGGPRAAGSSASISGHIRLVKGGSPR
                     PDASNAVVWIEGPRRDDGPGPALKMSQESKRFTPRVLIVPRKGTVEFPNNDPVYHNVF
                     SVSGTNRFDLGLYRSGASKSRSFGETGLVRVYCNIHPQMVGFLVVVDSDFAAITDRDG
                     AFRFEGVPPGSWTLKAWHEEGTETSAPLVVPSAGDSDLTVTIDTTAFRPVPHKNKYGK
                     DYPPLSGSDDERY"
     gene            2318..>3198
                     /locus_tag="VF958_13675"
     CDS             2318..>3198
                     /locus_tag="VF958_13675"
                     /inference="COORDINATES: protein motif:HMM:NF026177.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cache domain-containing protein"
                     /protein_id="HEX9150204.1"
                     /translation="MSATERSAPRLTTQPPPPGGGLKLPAPEPPRFSLSFKIFLAAAL
                     LILIAVGGAIAISAARSRAIADAKIDDDLKKAGPAWESFQRNRYEELHRALSIVVNNA
                     GIISMIAETDAGTVYDTLKREQASSARANFLIAIDPAGTAFARTDKPLPYSRDMRAVP
                     TIAAALQGEADAEGIWMSDHKLYHVVAAPVLQGGSQTVGVIAAGFQIDDDVASSLKSL
                     MNAEVVFLADAARLDEPARPFVAASTMHDTSPAVLSAVLATPRLVPAVLRDGKTIGPL
                     PLDVSGDTYLAYFLPIRS"
BASE COUNT          578 a         1068 c         1035 g          517 t
ORIGIN      
        1 agacgtcgcc ccgccagccg atgacgacga agacgacgca cgtcgccatc aaggtcgcga
       61 tcgtcatccc cgagatcggg ttcgaggacg tgccgatgat gccgacgatc cgcgacgaga
      121 ccgtcacgaa gaagaacccg aaaacgacga tcaggagcga catgagcaac gacccgggga
      181 acggcccgtg ggggaagccg ggcaggaccg ccaggatgag cacgatcgcg agagaaccga
      241 tcacgacgat tcccatcccc aggtcccggt ccgtccgctt gacaccctcc gccttctcga
      301 ctccgccccg gaagctcttg accgactcgc ggaaggcgga aacgatcgtc ggaagcgtct
      361 tgatgagcgt catcaccccg gcgcaggcga ccgcgccggc tccgatgtag cggatgtagg
      421 cgcggtagat ccagttcgcg tacgagttgt tggccatcca cgcgtcggag aacccgaggt
      481 tgtggaggtc ggtcaccacg cgggtctccg gcacgaagat cgcaatcaga ggaatcaagg
      541 ccatccacgc gaggagacct ccggaaaaga gctccgaagc gattcgaggt ccgatgatgt
      601 aaccgaggcc gaggtactcc ggagtcacgg cgacgtcgag cgtggcggcg gggtacgtcg
      661 gcgactcctt cttcgtgcgg aagaagatcg gcgtctcctg ccagagaccg agaatgccgt
      721 agaggaactt gtagaggaga gaggcgccga cgcccgcgaa gaccatcttc gcgaggttgc
      781 cgcccttctc ccctgcgatg aggacgtcgg cgcaggcggt cccctcgggg tacgggagct
      841 tgccgtgctc cttgacgatg agcgcgcggc gcagcggaat catgaagagg acgccgaggg
      901 ttccgccgaa gatcgcgagg atcgtgatct gaaggtaccg gaagaagttt tcgccgcccg
      961 acaggaagag gagcgccgga accgtgaaga ccacgccggc ggcaaccgac tcgccggccg
     1021 agccgatcgt ctgaacgatg ttgttctcga ggatcgtgga ctttccgatc ttcttgaaga
     1081 cggcgatcgc caggacggcg atcgggaccg aggcggaaac cgtcaggccg gcgcgaagcg
     1141 ccagatagac cgtcgccgcg ccgaagagga ttccgaagaa catcccgagg agaatcgcct
     1201 tgggcgtgaa ctcgggcatc gattgcccgg cggggacata gggctgaaat tccgcgccgg
     1261 acttctcggg aacgctcgcc atccggaccc tcctcttaag aagtggcggt cactgacttg
     1321 gcggcgatta tatgagtcgg gccgaccggc ctgcctacac ccctcacgtc cctccgaagc
     1381 cgatcgtgac ctggccgcgc tcgacgatga cgggaacgcg gcgcccgccg gacagctcga
     1441 ccatgcgcgc gagctctttc gcgtcccgtt tgacgtcgac gtatgtgaag ggaatcttcc
     1501 ggcgcgcgta gtcctcacgg gccgcggtcg tgtagggtca ggtgtctttc ccgaaaatct
     1561 ccacgcgatc gaattccatc ggtaactccc cgcacagatc ttaagtccgg ttccacgcta
     1621 tgattcaaaa cactcatgcg gacgcggttt ttcgccgccg cggcgctctt ctccgccctc
     1681 gtcccggcgg tcggaggacc gcgcgccgcc ggctcgtccg catccatctc cggccacatc
     1741 cgactcgtga agggcgggtc gccgcgcccc gatgcgtcga acgccgtcgt ctggatcgag
     1801 gggccgcgcc gcgacgacgg tccgggtccg gcgctcaaga tgtctcagga gtcgaagagg
     1861 ttcactccgc gcgtcttgat cgttccccgc aagggaaccg tggagttccc gaacaacgac
     1921 cccgtgtacc acaacgtgtt ctcggtgtcc gggacgaatc gcttcgacct cggcctctac
     1981 cggagcggag cgtcgaagag ccgctcattc ggcgagacgg gcctcgtccg cgtgtattgc
     2041 aacatccacc cgcagatggt cgggtttctc gtcgtggtcg actccgattt cgccgcgatc
     2101 acggaccgcg acggcgcctt ccgcttcgag ggcgtgccgc cggggtcgtg gacgttgaag
     2161 gcgtggcacg aggagggaac ggagacgagc gcccctctcg tcgttccctc tgctggcgac
     2221 tctgacctga cggtgacgat cgacacgacc gctttccgtc ccgttccgca caagaacaag
     2281 tacggcaagg actatccgcc gctgtcggga tcggacgatg agcgctactg aacggtcggc
     2341 gccgcgactg acgacgcagc ctcctccgcc gggcggcggg ctgaagctcc cggcgccgga
     2401 gcccccccgc ttctcgctgt ccttcaagat cttcctcgcc gcggcgctcc tgatcctgat
     2461 cgccgtgggc ggggcgatcg cgatctccgc cgcccgttcc cgcgcgatcg ccgatgccaa
     2521 gatcgatgac gacctcaaga aagcgggccc cgcctgggag tcgttccagc gaaaccgcta
     2581 cgaggagttg caccgcgcgc tcagcatcgt cgtcaacaac gcggggatca tctcgatgat
     2641 cgccgagacc gacgccggca cggtctacga cacgctgaag cgcgaacagg cgtcgagcgc
     2701 acgggcgaat ttcctgatcg cgatcgaccc ggccggaacg gctttcgccc ggaccgacaa
     2761 gccgctcccc tactcgcgcg acatgcgggc ggttccgacg atcgccgcgg cgctccaggg
     2821 cgaagcggac gccgaaggga tctggatgtc ggaccacaag ctgtaccacg tcgtcgcggc
     2881 gcccgtcctc cagggaggaa gccagacggt cggcgtgatc gcggccgggt ttcagatcga
     2941 cgacgacgtc gcgtcgagtt tgaagtcgct catgaacgcg gaggtcgtgt tcctcgcaga
     3001 cgccgcgcgg ctggacgagc cggcgaggcc gttcgtcgcc gcttcgacca tgcacgacac
     3061 gagtccggcc gtcctctcgg ccgtcctcgc cacgccgcgt ctcgttccgg ccgtgctgcg
     3121 cgacggaaag acgatcggcc ctctcccgct cgacgtctcc ggcgatacgt atctcgccta
     3181 cttccttccg atccgctc
//
LOCUS       DASWAV010000654         9271 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U2665
            k141_478800, whole genome shotgun sequence.
ACCESSION   DASWAV010000654 DASWAV010000000
VERSION     DASWAV010000654.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960316
            Sequence Read Archive: SRR10260007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 9271)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9271)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:47:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,929
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9271
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_478800"
                     /isolate="SMAG_U2665"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.36444 N 132.31889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..468
                     /locus_tag="VF958_13680"
     CDS             <1..468
                     /locus_tag="VF958_13680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9150205.1"
                     /translation="LRAPIVRLAGTCLLAFALSCSRRPPAPEPVPVGRVNCARCGMMI
                     SSERQSAEWVAPGEETRFYDDIGCLAADARTRPEGGVRFVHVTGARWAPAENAFYARP
                     PDESTPMGYGVVAFADRAAAAARDREGRARTWQELVRELGPEPPGSGGQPKPR"
     gene            529..1611
                     /locus_tag="VF958_13685"
     CDS             529..1611
                     /locus_tag="VF958_13685"
                     /inference="COORDINATES: protein motif:HMM:NF012596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HEX9150206.1"
                     /translation="MTTELYASDVSTAPAKPFHEILYETSDGVARVTINRPERFNAYS
                     TGCLRELAAAFQEASFDDSVGVIVLTGAKDHAFCTGGDVKEYAETYIGRPRDYWKYMA
                     LFRGYIDSILSTGKPVIARINGIAVGGGNESVLACDLAVMAGHAYLGQVGTRVGSVAC
                     GGATQWLPIVVGDKRAREMLFTNRRIPARLALEWGLVNRVAPTVRRATTWIENPTEDE
                     VRLARLRQDGWELDLSRLDAEVDGLARQLLEAFPECTRYTKQQTNFWKDLSWSLTARH
                     AQDWLALHYTDFEPFEGMNAFREKRPVDFAAVRRRAVEPGGSPETPWGAHVLACPGCG
                     ATGLPSRFEHCGVCGAPLGRSKTDSR"
     gene            1608..2357
                     /locus_tag="VF958_13690"
     CDS             1608..2357
                     /locus_tag="VF958_13690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013417738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX9150207.1"
                     /translation="MSSPRTLSGKVALVTGGAGGIGLACARQLRESGARVAIADSDGQ
                     RLRRLRRAFGSADLYEADVSDPSAARRIVLDLIGRRGRLDILVVAAGICRDAVLWKMT
                     DEAWSRVIDVDLSGAAHFLRAAAPVLRAQRSGRVVLVSSINGRRGKFGQANYAAAKAG
                     LTGLARSAAKELGPSGVTVNLVEPGFTETPMTAVLPPEVRQKAIAESALGRVGSAEDV
                     ANAVLFFVGPCAGHVTGQSLAVDGGQALGVG"
     gene            2357..3469
                     /gene="had"
                     /locus_tag="VF958_13695"
     CDS             2357..3469
                     /gene="had"
                     /locus_tag="VF958_13695"
                     /EC_number="1.1.1.368"
                     /inference="COORDINATES: protein motif:HMM:TIGR03201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0019439 - aromatic compound catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-hydroxycyclohex-1-ene-1-carbonyl-CoA
                     dehydrogenase"
                     /protein_id="HEX9150208.1"
                     /translation="MTSGVITGWEMSEPGGPMARTVRPMRHLEDGEVLVRVAGCGVCH
                     TDLGFLYDGVRTRSPAPLILGHEIVGWIEDSGGVHRDLVGRAVVVPSVIPCGECVACR
                     VGKPTICRRQVMPGNDCDGGFATHVILPGRALCPIPGASEDAGAPIGRARGLTLRHLA
                     VVADAVSTAYQSVERSGLVAGEIAIVVGLGGVGTYAAQFASLKGAAVVGVDVDPRPLQ
                     GATRIGVGLALDGRELQPKEARSRVLAFARSRGAPEIGWKIFECSGTPGGQKAAFSLL
                     VPGATILVVGFTRQPVEIPLSHLMAFDARAIGNWGCAPDLYPRILDYVLDGSVDVVSH
                     TELRPLARLREALEDIHSRRAVRRIVMTPNGDKEVA"
     gene            3466..4236
                     /locus_tag="VF958_13700"
     CDS             3466..4236
                     /locus_tag="VF958_13700"
                     /inference="COORDINATES: protein motif:HMM:NF012596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HEX9150209.1"
                     /translation="MSSPVRLSTERSGQIERIVLDRPSGNVLDLEMLAAIRARIEHIA
                     EEPGPCKLLVFEGTGRHFSFGASVAEHLPDKVGTLLPAFHRLFWDLERLSVPTAAVVR
                     GQCLGGGFELAVWCGLVFCDSTARLGVPEIKLGVFPPIAAIVLPWRMSGARSTQMILS
                     GESVDGSTAAELGLADSCSEDPEAELQKWFTANLEEKSAVAVRTAWRASRRLLAGALR
                     NDLTSLESLYLKDLMSHVDPIEGISAFVERRPPVWRHQ"
     gene            4233..5408
                     /gene="bcrC"
                     /locus_tag="VF958_13705"
     CDS             4233..5408
                     /gene="bcrC"
                     /locus_tag="VF958_13705"
                     /EC_number="1.3.7.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR02263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018522 - benzoyl-CoA reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="benzoyl-CoA reductase subunit C"
                     /protein_id="HEX9150210.1"
                     /translation="MSSRRAALVDWARAAAEDFEFTAAREWITERPGRKAAGYLPVYA
                     PREAIRASGLLPVGVHGAGEQVEIIRGDAFYQSYICHLPRSVIELAQSGRLSFLSAML
                     FPSTCDVIRNLSGVWQLLYPDLYVRYLDLPQIFDPRVALEFWAGEIRTLAADLGAISG
                     EPATDRTWREAIELQNRARSLVRKLYAARRDSPERIPTEELYLLLKAGEVVAVETFIE
                     KMEQYLAATEDSAVPLRDRARVIVVGAFCEQPPLGLVKTIERAGCSIVDDDFLIGNRL
                     FGDDVSVDGDPVVALAGAFLRNARATSTIYEPDPDAKRELMRRRVEAARADGIVFASA
                     SFCDPALLDRPMLRAGAEASGIACIAFQFAENTGQFQQFREQAGTFADSIKLWGAGQ"
     gene            5432..6679
                     /gene="bcrB"
                     /locus_tag="VF958_13710"
     CDS             5432..6679
                     /gene="bcrB"
                     /locus_tag="VF958_13710"
                     /EC_number="1.3.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008617886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018522 - benzoyl-CoA reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="benzoyl-CoA reductase subunit B"
                     /protein_id="HEX9150211.1"
                     /translation="MLLQKEMIAAHYRRLEQAPETGEPVVYTFVPGNLTELIRSFGAL
                     PVLPEINALQSAMRGKSQGYIATAERSGHSEDVCSYVKCDVGMLRSGNVGPTGTRLPK
                     PDLLLLSYTGCFTFMKWFELLREEYRCPTVFLHVPYQADGRETPEQRAYVTRQIRENL
                     LPALERATGRRYDEDRLRENLARSASSEDDLVAVLESAKRTPSPIDAYFGAVYYVGPI
                     FSAFRGTEEGGRYYSALREEVEARASQGLGPLTPSGPLDSQKYRLVVEGPPNWTHFRE
                     FWRMFSDEGAVVVASTYTKVGGTYDSGFRHDPRDPLGSLAEYCSGCYTNLSLPARVAM
                     LARYVDEYRADGFLIHSVKSCNSFSAGQLTILREVERRTGVPGAFIESDLVDARYFSP
                     ANLKTRLESYFQMLDARRAGVSA"
     gene            6676..7995
                     /locus_tag="VF958_13715"
     CDS             6676..7995
                     /locus_tag="VF958_13715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011384538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BadF/BadG/BcrA/BcrD ATPase family protein"
                     /protein_id="HEX9150212.1"
                     /translation="MKVFVGIDLGSTTTKAVVLDENGEIRGRGITNSRSNYDLAAELA
                     RTEAFVSARFDLLRREVEATAGKAALDRLLAAFRLEQRRAQLELLRERVGGEAALSAS
                     PADRPALLEILKALFDRIGAEEEERFENGDAGERSDFFRDAAGSAYARIAEELARPSG
                     AGRSADGAGPTPTPRGVSFDLLMGLYDKSIVAVENQPLELGFEDHVGPALRRIGNPPS
                     VRAGVDRAVSAEMEVAGEVGTGYGRARLPFPKEQIRSEILCHGLGAHVMFPGTRTVLD
                     IGGQDTKAIQVDAAGIVTSFQMNDRCAAGCGRYLGYIADEMNVGLHELGPIALASKRP
                     VRITSTCTVFAGAELRERISLGEKREDILAGLHRAVILRAMSLLARSGGIEDQFTFTG
                     GVARNPAAVAALERLVYENYGVRRINISPESIYTGALGAAHFARRAA"
     gene            7992..8840
                     /gene="bcrD"
                     /locus_tag="VF958_13720"
     CDS             7992..8840
                     /gene="bcrD"
                     /locus_tag="VF958_13720"
                     /EC_number="1.3.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004358393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018522 - benzoyl-CoA reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="benzoyl-CoA reductase subunit D"
                     /protein_id="HEX9150213.1"
                     /translation="MTVACGIDVGSSAVKVVVLAEEEGAITLLGRALARVRRRTLPQV
                     IEEAFGQAIDAAGVDAIDYVATTGEGEDVPFATGHFYGMTAHARGSLYLEPACRAVLD
                     VGALHSRAFAIDERGRVLRYKMTSQCASGSGQFLESIARYLGVAQAEIGALSLASVQP
                     ETVSSICAVLAETDVINMVSRGISAPDILRGLHDSMAGRLARLLQSVAADGVVFVSGG
                     LSADDGLVAALGRAFGKVAPGAKPVEIRRDPLSVYAGAIGAAIWGAFRFVKLGTRGVA
                     WKQATA"
     gene            8819..>9271
                     /locus_tag="VF958_13725"
     CDS             8819..>9271
                     /locus_tag="VF958_13725"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX9150214.1"
                     /translation="MEASDGITLRTLRASDGQRLSRIDERITGRNRSLWYERKLERAL
                     RDSDMNVSLGAEVEGVLVGAVLGSVQYGEFGIPEPVAVLDTILVDSLHARQGIATAML
                     EELASNLSRLGIDRIRTEIQWNDPLSRFLGSHGFALAPVLVLERSLSRE"
BASE COUNT         1385 a         3155 c         3183 g         1548 t
ORIGIN      
        1 ctccgggctc cgatcgttcg gctggcaggg acgtgtctcc ttgcgttcgc gctttcgtgc
       61 tcccgccgcc cgccggctcc cgagccggtt ccggtcggcc gggtgaactg tgcgcgctgc
      121 ggcatgatga tttcgagcga gcgtcagtcg gccgaatggg tcgcgcccgg cgaagaaacg
      181 cgcttctatg acgacatcgg atgcctcgcg gccgacgccc gaacgcgtcc ggaagggggc
      241 gtgcgattcg tccacgtgac cggagcgcga tgggcgccgg cggagaacgc cttctacgct
      301 cgtcccccgg acgaatcgac acccatgggc tacggcgtcg tcgctttcgc cgaccgggcg
      361 gcagcggcgg cccgcgaccg ggagggacgg gcccgcacct ggcaggagct cgtccgagag
      421 ctcggtcccg agccgccggg atccggaggt cagccgaaac ctcggtgacc tcagggactg
      481 atgcttttgc accactccga cggcgcagca ggctcctagc atcgcctcat gacgacagag
      541 ctttacgcgt cggatgtctc gacggcgccg gcgaagcctt tccacgaaat cctctacgag
      601 acctccgacg gcgtcgcccg cgtcaccatc aaccggcccg agcgattcaa cgcgtactcg
      661 accggctgct tgcgggagct cgccgccgcg ttccaggagg catccttcga cgattcggtc
      721 ggggtgatcg ttctgaccgg cgcgaaggac catgcgttct gcacgggggg cgacgtcaag
      781 gaatacgcgg agacgtacat cggacgcccg cgggactact ggaagtacat ggcgctcttc
      841 cgcgggtaca tcgactcgat cctctcgacg ggcaaaccgg tgatcgcacg catcaacggg
      901 atcgccgtcg gcggcggcaa cgaaagcgtt ctggcgtgtg acctcgccgt gatggccggc
      961 cacgcctacc tgggccaggt cggaacgcgc gtcggttcgg tcgcgtgcgg gggcgccacc
     1021 cagtggctgc cgatcgtcgt gggggacaag cgcgcgcggg agatgctctt caccaaccgc
     1081 cgcattcccg cacggctcgc cctcgagtgg ggcctcgtca atcgggtggc tcccacggtc
     1141 cggcgcgcga cgacgtggat cgagaacccg acggaagacg aagtccggct cgcgcggctc
     1201 cggcaggacg gttgggagct cgatctgtcg cgtctcgacg cggaggtcga tggtcttgcc
     1261 cgtcagcttc tcgaagcgtt tcccgagtgc acccgctaca cgaagcagca gacgaacttc
     1321 tggaaggacc tttcctggtc gctgacggcg cggcacgcgc aggactggct cgcgctccac
     1381 tacaccgact tcgagccgtt cgaggggatg aacgcgtttc gcgagaaacg tccggtcgac
     1441 ttcgcggccg ttcggcggcg cgccgtcgaa ccgggcggct cgcccgagac accgtgggga
     1501 gcgcacgtcc tcgcgtgccc cggttgcggc gcaaccggtc tcccttcccg attcgagcac
     1561 tgcggcgttt gcggtgcgcc gctcggccgg tcgaagacgg acagccgttg agcagtcctc
     1621 ggaccctctc cggcaaggtc gccctcgtca ccggcggcgc cggcggcatc gggctcgcgt
     1681 gcgcccgaca gctgcgcgaa agcggcgcac gagtcgcaat cgcggacagc gacgggcaac
     1741 gtctccgacg tctgcggcgt gcgttcggct ctgcggacct ctacgaggcg gacgtctccg
     1801 acccgtccgc ggcgcgccgg atcgtcctcg atctgatcgg ccgccgtgga cgcctcgaca
     1861 tcctcgttgt cgccgccgga atctgccgcg acgccgtgct ctggaagatg acggacgagg
     1921 catggtcgcg cgtgatcgac gtggacctct cgggcgccgc ccacttcctg cgagcagccg
     1981 cgcccgtgtt gcgcgcgcag cgctcgggcc gcgtggtcct cgtttcctcg atcaacggcc
     2041 gccgggggaa attcggccag gcgaactatg cggccgccaa ggcggggctg accgggctcg
     2101 ctcgatccgc ggcgaaggag ctgggcccct cgggcgtaac cgtcaacctc gtcgagccag
     2161 gcttcaccga gacgccgatg accgccgtcc tccctccgga ggttcggcag aaggcgatcg
     2221 cggagagcgc cctcggccgc gtcgggtccg cggaagacgt cgccaatgct gttcttttct
     2281 tcgtgggccc ctgtgcgggc cacgtcaccg gccagtccct cgccgtcgac gggggacagg
     2341 cgctgggggt tgggtaatga cgagcggcgt gatcacgggc tgggagatgt ccgagccggg
     2401 cggtcccatg gcgcgcaccg tccgtccgat gcggcacctc gaagacgggg aggttctcgt
     2461 gcgcgtcgcc ggttgcggcg tgtgccacac ggacctcggc tttctctacg acggtgttcg
     2521 cacgcggtct cccgcgccgc tgattctcgg acacgaaatc gtcggctgga tcgaggattc
     2581 gggcggcgtc caccgggatc tcgtcggccg cgcggtcgtc gtgccgtcgg tcatcccgtg
     2641 cggcgagtgc gtcgcgtgcc gcgtcggaaa accgacgatc tgccgccggc aggtcatgcc
     2701 ggggaacgac tgcgacggcg gtttcgcgac ccacgtgatc ctccccggcc gcgccctgtg
     2761 tcccattccc ggagcgagcg aagacgccgg ggcgccgatc ggtcgggcgc ggggcctgac
     2821 gctccgccac ctggccgtcg tcgccgacgc cgtctcgacg gcttaccagt cggtcgagcg
     2881 ttcgggtctc gtcgcgggtg agatcgcaat cgtcgtcggc ctgggaggcg tcgggaccta
     2941 cgcggcgcag ttcgcctctc tgaagggtgc ggcggtagtg ggagtcgacg ttgatccgcg
     3001 cccgctgcag ggcgcgaccc ggatcggagt cgggctcgcg ctcgacggtc gcgagctcca
     3061 gcccaaggaa gctcgctccc gcgttctcgc gtttgcgcgc tcccggggag cgccggagat
     3121 cggctggaag atcttcgagt gctcgggaac gccgggcgga cagaaagccg ccttctcgct
     3181 gctcgtgccg ggcgcgacga ttctcgtcgt ggggttcacg cgtcagccag tcgagatccc
     3241 gctctcgcac ctgatggcgt tcgacgcccg cgcgatcggc aactgggggt gcgccccgga
     3301 cctctaccct cggatcctcg attatgtgct ggacggatcg gtggacgtgg tctcgcacac
     3361 cgagctccgg ccgctggcgc gccttcgcga ggccctcgaa gacatccaca gccgccgagc
     3421 cgtgcggcgg atcgtcatga cacccaacgg agacaaggag gtcgcatgag ctctcccgtt
     3481 cgcctttcga ccgaacgctc gggacagatc gagcgcatcg tgctcgatcg ccccagcggc
     3541 aacgtcctcg atctcgagat gctcgccgcg atccgggcga ggatcgagca cattgcggag
     3601 gagccggggc cgtgcaagtt gctcgttttc gaagggaccg gacggcactt ctccttcgga
     3661 gcgtccgttg ccgaacacct cccggacaag gttggaactc tccttcccgc cttccatcgc
     3721 ctcttttggg acctagaaag gctctcggtc ccgacggcgg cggtcgttcg cggccagtgc
     3781 ctcggcgggg gattcgagct cgctgtctgg tgcgggctcg ttttctgcga ctcgaccgct
     3841 cgtctcggcg tacccgagat caagctcgga gtctttcccc cgatcgccgc gatcgtcctg
     3901 ccctggagaa tgagcggtgc gcgctcgacg cagatgatcc tctcgggaga gtccgtggac
     3961 ggcagcaccg cggcggagct cggtctggcg gactcgtgca gcgaagaccc cgaggccgag
     4021 cttcagaagt ggttcaccgc gaacctcgag gagaaatcgg cggtcgccgt tcgtacggcg
     4081 tggcgcgcgt cccggagact gctcgccggc gcgctccgca acgacctgac gtccctcgag
     4141 tcgctctacc tgaaagatct gatgtcacac gtggatccga tcgagggcat ctccgcgttc
     4201 gtggaaagac ggccgcccgt ctggagacat cagtgagctc tcgccgcgcg gcgctcgtcg
     4261 actgggcgcg cgcggcggcc gaggacttcg agttcaccgc ggcgcgcgaa tggatcaccg
     4321 agcggccggg ccgcaaggcg gccggatatc tcccggtcta tgcgccacgc gaagcgatcc
     4381 gcgcgagcgg cctcctgccg gtcggtgttc acggcgccgg cgagcaggtc gagatcatcc
     4441 gcggagacgc tttctaccag tcgtatattt gccacctccc gcgctcggtc atcgagctcg
     4501 cgcagtccgg acggctgtcg tttctgtccg cgatgctctt tccttcgact tgcgacgtca
     4561 tccggaatct ctccggcgtc tggcagctcc tttacccgga cctgtacgtg cgctatctcg
     4621 acctcccgca gatcttcgat ccgcgcgtcg ccctcgagtt ctgggcgggc gagatccgga
     4681 ctctcgccgc ggatctcggc gcgatttccg gcgagccggc gacggatcgg acctggcgcg
     4741 aggcaatcga gctccagaac cgcgctcgct cgctcgtgcg aaagctctac gccgcgcggc
     4801 gtgattcccc cgagcggata ccaaccgagg agctctacct cctcctcaag gcgggtgagg
     4861 tcgtcgccgt ggaaacgttc atcgaaaaaa tggagcagta ccttgctgcg accgaggaca
     4921 gcgccgttcc tctccgggac cgcgctcgcg tgatcgtcgt cggggcgttc tgcgagcagc
     4981 cgccactcgg gctggtcaag acgatcgagc gggccggttg ctcgatcgtg gacgacgatt
     5041 tcctgatcgg caaccgcctg ttcggcgacg acgtgtcggt cgacggagat ccggtcgtcg
     5101 cgctcgcggg ggctttcctg cgcaacgccc gggcgacctc cacgatctac gagccggacc
     5161 cggacgccaa gcgtgagctc atgcggcggc gggtcgaggc cgctcgagcc gacggaatcg
     5221 tcttcgcgtc cgcgagcttc tgcgatcccg cgctgctcga ccggccgatg ctgcgcgctg
     5281 gcgcggaggc ctccggcatc gcgtgcatcg cattccaatt cgcggaaaac accggccagt
     5341 tccagcagtt ccgcgagcag gcgggaacgt tcgcggactc gatcaagctc tggggcgccg
     5401 ggcagtgagc gtcgagacga aggaccgctc catgctcctg cagaaggaga tgatcgccgc
     5461 gcactaccgc cggctcgaac aggcccccga gaccggagag ccggtcgttt acaccttcgt
     5521 ccccgggaac ctgacggagc tcatccgatc gttcggcgcg ctcccggttc tgccggagat
     5581 caacgccctg cagtccgcga tgcgcgggaa gagccagggt tacatcgcga ccgccgagcg
     5641 ctcgggccac tcggaggacg tctgctcgta cgtgaagtgc gacgtcggca tgctgcggtc
     5701 cggcaacgtg ggcccgaccg ggacgcgtct gccgaagccc gatctcctgc tgctctccta
     5761 caccggctgc ttcaccttca tgaagtggtt cgagctcctc cgcgaggagt accgctgtcc
     5821 gaccgtcttc ctccacgtcc cctatcaggc ggatggccgg gaaacgccgg agcagcgcgc
     5881 ctacgtcacg cgtcagatcc gcgagaacct ccttccggcg ctggagcgcg cgaccgggag
     5941 gaggtacgac gaagaccggc tgcgggagaa cctggcgcgg agcgcgtcgt cggaggacga
     6001 tctcgtcgcg gttctcgagt ccgcgaagcg gacgcccagc cctatcgacg cgtacttcgg
     6061 cgcggtgtac tacgtcgggc cgatcttcag cgcgttccgc ggaaccgagg agggcggccg
     6121 ctactattcc gctctgcgcg aggaggtgga ggcgcgcgca tctcagggac tcgggccgct
     6181 caccccctcc ggcccgctcg actcccagaa gtaccggctc gtcgtcgaag ggcctccgaa
     6241 ctggacccac ttccgggagt tctggaggat gttctccgat gagggcgcag tcgtcgtcgc
     6301 ctcgacctac acgaaggtcg gcgggacgta cgactcggga ttccgccacg atccgcgcga
     6361 tcccctggga agcctcgccg agtactgcag cggctgctac acgaaccttt cgctgccggc
     6421 gcgggtcgcc atgctcgccc gatacgtcga cgaatatcga gccgatgggt tcctgatcca
     6481 ttcggtgaaa tcgtgcaact cgttctcggc gggacagctg acgatcctgc gcgaggtcga
     6541 gcgccgaacg ggagtccccg gcgccttcat cgaatccgac ctggtggatg cgcgctactt
     6601 ttctccggca aacctgaaaa cccgcctcga gagttacttc cagatgctcg acgcccgccg
     6661 cgcgggggtg tcggcgtgaa ggtcttcgtc ggaatcgacc tgggttcgac gacgacgaag
     6721 gccgtcgttc tggacgagaa cggagagatc cggggccgcg gcatcaccaa ctcgcggtcc
     6781 aactacgacc tcgccgcgga gctggcgcgg accgaggcgt tcgtctccgc tcgcttcgac
     6841 ctgctgcgcc gcgaagtgga ggcgacggcc ggaaaagcag cgctcgaccg tctgctcgcc
     6901 gcgttccggc tcgagcaacg gcgcgcgcag ctcgagctcc tccgcgagcg cgtcggagga
     6961 gaggcggcgc tctccgcctc cccggcggat cggcccgcgt tgctcgagat cctgaaggct
     7021 ctcttcgatc ggatcggagc ggaagaggag gagcgcttcg aaaacggcga tgccggggaa
     7081 cgctccgatt tcttccgcga tgcggccggc tccgcgtacg cgcggatcgc cgaggagctc
     7141 gcgcggccct cgggcgccgg gagatcggcc gacggtgcgg gtccgacgcc gacgccgcgc
     7201 ggcgtgagct tcgacctgct gatgggcctc tatgacaagt cgatcgtcgc ggtggagaat
     7261 cagccgctgg agctcggttt cgaggaccac gtcggtccgg ctctgcgccg catcggaaat
     7321 ccgccctcgg tgcgcgcggg tgtcgaccgc gcggtctccg cggagatgga ggtcgccgga
     7381 gaggtcggca cgggctacgg ccgcgcccgg ctgcccttcc cgaaggagca gatccgttcc
     7441 gagattctct gccacggact cggcgcgcac gtcatgtttc cggggacccg cacggtgctc
     7501 gacatcggcg ggcaggacac gaaggcgatc caggtggacg ccgccggaat cgtgacgagc
     7561 ttccagatga acgaccgctg cgccgcgggt tgcggtcgtt atctcggcta catcgccgac
     7621 gaaatgaacg tggggctgca cgagctcggg ccgatcgcac tcgcctcgaa gcgtccggtt
     7681 cgaatcacgt ccacgtgcac ggtgttcgcg ggcgccgagc tccgcgagcg catttcgctc
     7741 ggcgagaagc gcgaggacat actcgcgggc cttcaccgtg cggtgatcct tcgggcgatg
     7801 agtctcctcg cgaggtcggg cggaatcgag gaccagttca ccttcaccgg cggagtcgcg
     7861 cgcaacccgg cggccgtcgc agcgctcgag cgactcgtct acgagaacta cggcgtgcgt
     7921 cggatcaaca tctctccgga aagcatctac acgggcgcgc tcggcgccgc ccacttcgcc
     7981 cggagggcgg cgtgaccgtc gcgtgcggca tcgacgtcgg atcgagcgcc gtcaaggtgg
     8041 tcgtgctggc cgaggaggaa ggcgcgatca cgcttctcgg ccgcgcgctc gcccgcgtgc
     8101 gacgccggac gctcccgcag gtcatcgaag aagccttcgg gcaggcgatc gacgcggccg
     8161 gtgtcgacgc gatcgactac gtcgcgacga cgggagaggg ggaggacgtc ccgttcgcga
     8221 ccggccactt ctacgggatg accgcgcacg cgcgcgggtc tctgtacctc gagccggcct
     8281 gccgcgccgt tctcgacgtc ggcgcgctcc actcccgggc gtttgcgatc gacgagaggg
     8341 gacgagtgct tcgctacaag atgacgagcc agtgcgcctc cggctccggt cagttcctcg
     8401 agtcgatcgc gcggtatctc ggagtcgcgc aggcggagat cggcgccctg tctctcgctt
     8461 cggtgcaacc cgaaacggtg agctcgatct gtgcggtgct cgcggagacc gacgtcatca
     8521 acatggtgtc tcgcgggatc tcggcgcccg acatcctccg cggcctgcac gacagcatgg
     8581 ccgggcgcct cgcacggctg ctccagtcgg tcgcggccga tggagtcgtg ttcgtcagtg
     8641 gcggcctgtc ggccgacgat gggctcgtcg ccgcgctcgg tcgcgcgttc ggaaaggtcg
     8701 ctcccggcgc gaagccggtc gagatccgac gggatcccct ttcggtctat gcgggtgcga
     8761 tcggagcggc gatctggggg gctttccggt tcgtcaagct cggaacgaga ggtgtggcat
     8821 ggaagcaagc gacggcataa ctcttcgaac gctccgcgcg tcggacggac aacgcctgtc
     8881 ccggatcgac gagcgcatca ccggaagaaa ccggagtctc tggtacgaac gcaagctcga
     8941 gcgggccctg cgcgattcgg acatgaacgt gtcgctcggt gcggaggtcg agggcgtcct
     9001 cgtcggagca gtgctcgggt ccgtccagta cggcgaattc ggaatcccgg agccggttgc
     9061 ggtgctcgac acgatccttg tggactctct ccacgcccga caggggatcg caacggcgat
     9121 gctcgaggag ctcgcgtcga acctctcccg cctcggcatc gatcgcatac gtacggaaat
     9181 ccagtggaac gacccattgt cccggttcct cggaagtcac gggttcgcgc tcgcgcccgt
     9241 cctcgtcctg gagcgctccc tctcccgcga g
//
LOCUS       DASWAV010000655         4919 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U2665
            k141_77997, whole genome shotgun sequence.
ACCESSION   DASWAV010000655 DASWAV010000000
VERSION     DASWAV010000655.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960316
            Sequence Read Archive: SRR10260007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 4919)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4919)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:47:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,929
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4919
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_77997"
                     /isolate="SMAG_U2665"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.36444 N 132.31889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..556)
                     /locus_tag="VF958_13730"
     CDS             complement(<1..556)
                     /locus_tag="VF958_13730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9150215.1"
                     /translation="MVAQTTLQAIPPDRLTAFVKSGTGQPEELDLLAREAADPFVLEE
                     IVRSRSTRDETLLYLAKTVSGRPQDALIANQARLLAEPALIRALFENPDLTADGRRLL
                     TELTEEFFEKRVRRRESEARSRESESAAPHEELSPPEGVDLDADLDLLDDREEASPEA
                     SAAGEEAPWDAEENSLFIGAIYKRL"
     gene            complement(726..1508)
                     /locus_tag="VF958_13735"
     CDS             complement(726..1508)
                     /locus_tag="VF958_13735"
                     /inference="COORDINATES: protein motif:HMM:NF024855.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RodZ domain-containing protein"
                     /protein_id="HEX9150216.1"
                     /translation="MQRSRPSVPVFGEELKRERNLREISLEEIAAATKISMRLLTALE
                     ASDLSRLPAPVFTRGFIRAYCLYLGLDPVQKINTYLAEVQPSAAPQETSVAKPRSGSR
                     FLRGPRSTAGTIVGGVTAVLLLLGLIANPAHHRPNVARASRVALVSPVSFKNVAPSSE
                     PTPLIREDAPETTLTSEGITLVLEFDESSWVEISADGTKVFGGLVTAGESRRFEAKKE
                     FRLTLGNAGGVRVSIDGHPAARLGRPGEVIRDFRLPDPPSRG"
     gene            complement(1562..2266)
                     /locus_tag="VF958_13740"
     CDS             complement(1562..2266)
                     /locus_tag="VF958_13740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9150217.1"
                     /translation="MPTARTRKKTPKPEVAVGVEGQFLRELRRPDLAAADVERILRNP
                     DARRFHAVRLAIAGHRNTPRMQALSLVGTLYWRDLAHLSADVRVHPEIRRSADAHLLR
                     RLPELAVAERIDLARVAGRGALLMLRQDPDPRVVAAFLDNRFATEVDVVQAAALSRAR
                     PESLAGIAGHPRWSVRRSVREALLANPNLPEAAAEAILDRSTDRELDLLQSEPAARED
                     VRKIAQRVLARRLRQV"
     gene            complement(2221..3069)
                     /gene="trpA"
                     /locus_tag="VF958_13745"
     CDS             complement(2221..3069)
                     /gene="trpA"
                     /locus_tag="VF958_13745"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: protein motif:HMM:TIGR00262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HEX9150218.1"
                     /translation="MSRINAAFARARREGRAAFVPYLTAGDPSAAGTVSLARVLDQAG
                     ADVLELGVPFSDPVADGPVLQRSAARALASGTNFPAVLRIAESIRAETDLAMVLFSYL
                     NPLLRRGLARAAREARAVGFDAVLVTDVPADEGAEIGGVFRREGLDTVFLVSPTSPAS
                     RMEQAARISSGFLYVISRRGTTGARDSLPADLRATLTRARRAVKMVTRRSEEPSLPIA
                     VGFGIGTPRDAKAAAALADGVVVGSALVALAEKTGDDEQVGRFAESLVRACRRPGPAR
                     RLQSPK"
     gene            complement(3066..4244)
                     /gene="trpB"
                     /locus_tag="VF958_13750"
     CDS             complement(3066..4244)
                     /gene="trpB"
                     /locus_tag="VF958_13750"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012464366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HEX9150219.1"
                     /translation="MSRSARRGYFGAYGGRFAPETLLAPLEELERAFERYRRDAAFRR
                     ELGGLLRNYAGRPTPLSAAPRLSAISGGARIALKREDLLHTGAHKLNNCLGQVLLAKR
                     MKKRRVIAETGAGQHGVATAAAAARLGLPCRVYMGAVDMKRQSPNVSRMRLLGAEVVA
                     VESGSRTLKDAISEAMRDWIENVATTHYVLGSALGPHPYPTMVREFQSVIGREARAQF
                     RRLAGRDPDVAVACVGGGSNAIGLFSAYLESRVRLYGVEAGGRGRGPGEHAARFAGGS
                     VGVLHGTWTMLLQDADGQILPTHSISAGLDYPAVGPEHAYLESVGRVTYAKVSDKEAL
                     DAFRQLARTEGILPALESAHAIAYALKLARRLPKRSWILVNLSGRGDKDLDIVESAPH
                     "
     gene            complement(4241..>4919)
                     /locus_tag="VF958_13755"
     CDS             complement(4241..>4919)
                     /locus_tag="VF958_13755"
                     /inference="COORDINATES: protein motif:HMM:NF012900.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phosphoribosylanthranilate isomerase"
                     /protein_id="HEX9150220.1"
                     /translation="GVAQSRRAGGSAAGARAVTDVKVCGLTREGDVESACALGAAYVG
                     FNFSAGSPRRVPLDVARRLAAVVPEGVPRVGVFVDESYAEIAEAIDAASLDLVQLHRT
                     LSAEDLKRIPRRVLAVAHAGRHEEIPPGALLERCAGILFDTARSGRAGGTGIPFDWFL
                     IEGKSWPVPLFVAGGLTAENVGESIRMTKPGAVDVASGVETSPGVKDLELMRRFFSAV
                     READSKP"
BASE COUNT          795 a         1683 c         1705 g          736 t
ORIGIN      
        1 cgaggcgctt gtagatcgcg ccgatgaaga gagaattctc ctcggcgtcc caaggagctt
       61 cctctccggc ggccgacgcc tcgggactcg cctcttcgcg gtcgtcgagg agatcgaggt
      121 ccgcatccag gtcgacacct tcgggcggcg acagttcctc gtgcggcgcg gcgctctccg
      181 actcccggct ccgcgcttcc gactcccggc gccgcacgcg cttctcgaag aactcctcgg
      241 taagctcggt gagaagccga cgcccgtccg ccgtcaggtc cggattctcg aacagcgcgc
      301 gtatcagcgc cggctcggcg aggagacgtg cctggttggc gatcagggcg tcctgcggac
      361 ggccggacac cgtcttcgcg aggtagagca gagtctcgtc ccgagtggac cgcgagcgca
      421 cgatctcctc gaggacgaac gggtccgccg cttcgcgggc gaggagatcg agctcctccg
      481 gctggcccgt tcccgacttg acgaatgccg tgagtcggtc cggagggatt gcctgcaggg
      541 tggtctgcgc gaccattgcg gtctccggat cgggccgtcc cagcacggcg aaaagggccc
      601 ggagggaatc ctccggatcg agcgggagaa gacctcgcgc ggcgcgcaga cggatctcgg
      661 cgggcgcaag cccgctccgc agaagtgtga cgaggtcttc ggaaggaatg ggggtcgggt
      721 tcgctctagc ctcgcgacgg gggatcgggc agacggaagt cccggatgac ttcgccgggg
      781 cgaccgaggc gagccgccgg atggccgtcg atcgaaactc tcactccgcc ggcgttgccg
      841 agcgtcaaac ggaactcttt cttcgcctcg aagcgacggc tctcgccggc ggtgacgaga
      901 ccgccgaaaa ctttcgtccc gtcggcactg atctccaccc aggaggattc atcgaactcg
      961 agaacgagcg tgatcccctc cgaagtgagc gttgtctccg gagcgtcctc acggatgagc
     1021 ggcgtcggtt cgctcgaggg cgcgacgttc ttgaaggaga ccggtgagac gagggcgacg
     1081 cgtgacgcac gagcgacgtt cggccggtgg tgggccggat tcgcgatgag gccgaggagc
     1141 agcagcaccg cggtcactcc accgacgatc gtaccggccg tcgagcgcgg tcccctcaga
     1201 aagcgggagc cgctccgcgg cttcgcgacg gacgtctcct gcggcgccgc actcggctgc
     1261 acttccgcca gataggtgtt gatcttctgg acggggtcaa gtccgaggta gagacagtag
     1321 gcgcggatga atccccgcgt gaagacaggg gcaggaagtc gggagaggtc actcgcttcg
     1381 agcgccgtca agaggcgcat cgagatcttg gttgccgcgg cgatctcctc aagggagatc
     1441 tcgcgcaagt ttcgttcgcg cttgagctct tccccgaaga ccggaaccga cggtcgagaa
     1501 cgttgcatcc gtcgagaaaa cctgttcttt tttttagatc cgaagtgggt tcgattccat
     1561 gctagacctg cctcagccgt cgcgcaagga ctctttgagc aatctttcgg acgtcctccc
     1621 gagcggcggg ctcgctctgc aggagatcga gctcccgatc ggtggagcgg tccaggatcg
     1681 cctcggcggc ggcttcggga agattcggat tcgcgagcag ggcctcacgt acggaccgac
     1741 ggacgctcca ccgggggtgc ccggcgatcc cggcgagcga ctcgggccgc gcccgggaga
     1801 gcgccgccgc ctgcacgacg tcgacttcgg tcgcgaagcg gttgtccagg aacgccgcga
     1861 cgacccgcgg atccgggtct tgccggagca tgagcagggc gccgcggccg gcgacccggg
     1921 cgaggtcgat ccgctcggcg accgccagct ccggaaggcg ccggaggagg tgcgcatccg
     1981 ccgacctgcg gatttccggg tgcacacgga catccgccga caggtgagcc agatcacgcc
     2041 agtagagcgt cccgacgagc gagagcgctt gcatccgggg ggtgttccga tggccggcga
     2101 tggcaagacg cacggcgtgg aaacgacggg cgtcggggtt tcggaggatc cgctcgacgt
     2161 cggcagccgc caggtccggc cggcgcagct cccggagaaa ctgcccttcg acccccaccg
     2221 ctacttcggg ctttggagtc ttcttgcggg tccgggccgt cggcacgcgc ggacgaggct
     2281 ctccgcgaag cggccgacct gctcatcgtc acccgttttc tcggccagcg ccaccagcgc
     2341 cgagcccacg acgacgccgt cagcgagcgc ggcggcggct ttggcgtcgc gcggcgtccc
     2401 gatcccgaag ccgactgcga tcggcagcga cggctcctcc gaacgtcgcg tcaccatctt
     2461 cacggcgcgt ctcgcgcgcg tgagggtcgc acgcaggtcc gccggcagcg agtctcgcgc
     2521 gccggtcgtg ccccgccgcg agatcacgta gagaaacccg ctcgagatcc gggccgcctg
     2581 ttccatccgc gaagccggcg acgtcggcga cacgagaaaa actgtgtcga gaccctctct
     2641 gcgaaagacg cccccgattt cagcgccttc gtccgccgga acgtccgtca cgaggaccgc
     2701 gtcgaatccg acggcacgtg cctctcgagc ggcccgcgcc aggccccggc gcaggagcgg
     2761 gttcaagtac gagaagagga ccatcgcgag gtccgtttcg gcgcggatag attcggcgat
     2821 tcgcagaact gccgggaagt tcgttccgct ggcgagggcc cgcgccgccg atctctggag
     2881 aacggggccg tcggcaaccg ggtcggagaa cggcacaccg agctcgagca cgtcggcccc
     2941 ggcctggtcg agaacccggg cgagtgacac ggttccggcg gccgaggggt cgcccgccgt
     3001 gaggtaggga acgaaggccg cgcgtccttc ccgacgagcg cgagcgaagg cggcgtttat
     3061 ccggctcaat gcggtgcgga ctcgacgatg tcgaggtcct tgtcgccccg ccccgagaga
     3121 ttgacgagga tccacgaccg ttttggcaga cgccgggcga gcttcagcgc gtaggcgatc
     3181 gcgtgcgcgc tctcgagcgc cggaagaatc ccttcggtcc gcgcgagctg gcgaaaggca
     3241 tccagggctt ccttgtccga gaccttcgcg taagtcacgc ggcccactga ctcgaggtac
     3301 gcatgctccg gcccgaccgc cgggtagtcg agccccgccg agatcgagtg cgtcggaagg
     3361 atctggccgt cggcgtcctg caggagcatc gtccacgtcc cgtggagcac gccgacggat
     3421 ccgccggcga accgcgccgc atgctcaccc ggaccgcggc cccggcctcc ggcttcgacc
     3481 ccgtagaggc gcacgcgcga ctcgaggtac gcggagaaaa gcccgattgc attggacccg
     3541 ccgcccacac aggcgacggc aacgtcgggg tcgcgccccg ccagccggcg gaactgcgcc
     3601 cgggcctctc gaccgatgac ggactggaac tcacgaacca tcgtcggata ggggtgcggt
     3661 ccgagggcgg agccgaggac gtaatgggtc gtcgcgacgt tctcgatcca gtcgcgcatc
     3721 gcctcactga tcgcgtcttt cagagttcgg gagccggatt cgaccgcgac gacctccgca
     3781 ccgagcagtc gcatcctcga gacgttgggc gattggcgct tcatgtccac ggcgcccatg
     3841 tacacgcggc acggaaggcc gagccgcgcg gccgccgcgg cggtcgcgac cccgtgctga
     3901 cccgcgccgg tttccgcgat cacgcgcctc tttttcatcc gcttcgcgag caggacctgc
     3961 cccaggcagt tgttcagctt gtgcgcgccc gtgtggagga ggtcctcgcg cttcaaggca
     4021 attcgcgcgc cgccggagat cgcagagagc cgcggcgccg ccgacagagg cgtcgggcgt
     4081 ccagcgtagt tgcgcaggag accgccgagc tcacggcgga aggccgcgtc gcgccggtac
     4141 cgctcgaacg cgcgctcgag ctcttcgagc ggcgccagga gcgtctcggg cgcaaaacgg
     4201 ccgccgtagg cgccgaaata gccgcgccgc gcggaccggc tcatggcttc gagtccgcct
     4261 cccgcacggc ggagaagaag cggcgcatca gctcgaggtc cttgaccccc ggagaagtct
     4321 cgacgccgga cgcgacgtcc acggcgcccg gtttcgtcat ccgaatggac tctccgacgt
     4381 tttcggcggt gaggccgccc gccacgaaca gcgggacggg ccagctcttc ccttcgatga
     4441 ggaaccagtc gaacggtatc cccgtgcccc ccgcacgccc gcttcgggcc gtgtcgaaga
     4501 ggatccccgc acacctctcg agcagcgcgc ccggagggat ctcttcgtga cgaccggcgt
     4561 gtgcgacggc gaggacgcgg cgcgggatcc gcttcaggtc ttcggcggag agcgtgcggt
     4621 ggagttggac gagatcgagt gaagccgcat cgatcgcctc ggcgatctcg gcgtagctct
     4681 catcgacgaa cacgccgacg cgggggacac cctccggcac gacagccgcg agccgcctcg
     4741 ccacgtcgag agggacgcgg cgcggcgacc cggccgagaa attgaacccg acgtacgcgg
     4801 cgccgagcgc gcacgcggac tcgacgtccc cctcgcgtgt cagaccgcag accttgacgt
     4861 ccgtcaccgc gcgagctccc gcagcgcttc ctccggctct tcggctttga gcaacgcct
//
LOCUS       DASWAV010000656         2925 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U2665
            k141_557979, whole genome shotgun sequence.
ACCESSION   DASWAV010000656 DASWAV010000000
VERSION     DASWAV010000656.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960316
            Sequence Read Archive: SRR10260007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2925)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2925)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:47:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,929
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2925
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_557979"
                     /isolate="SMAG_U2665"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.36444 N 132.31889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..669)
                     /locus_tag="VF958_13760"
     CDS             complement(<1..669)
                     /locus_tag="VF958_13760"
                     /inference="COORDINATES: protein motif:HMM:NF024091.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HEX9150221.1"
                     /translation="MRAWAASVEEVFREASARWTLLAYFALSTLFIMIFALAVNLDIV
                     DGALAGAKLFGKELDLGGGKIDMDQLVLGFETGFSGVLFMLGTFLALFATAHLVPRLQ
                     EKGTIDLYLSRPVGRTKLLLSRYSGGLLLAAANLLYLIGSIWLIVYWKTGVAHPRFLL
                     GGVVIFFTIACLVAFAFLVGVVTSSTAVSLMVTYAIFFFAALLSAREKIAAAVSTEFS
                     ANLVR"
     gene            complement(666..1610)
                     /locus_tag="VF958_13765"
     CDS             complement(666..1610)
                     /locus_tag="VF958_13765"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX9150222.1"
                     /translation="MAPVIQADGLRKVYRSRFGTRRVTALDGIDLSVGPGEVFGLLGP
                     NGAGKTTAVKILLGLTQPTEGSARLFGIPAADPESRRRVGYLPEGHRFPGYLTARQTL
                     SIFGRMSGMERTALKTRIPDLLARVRLSEWADVKVKRFSKGMTQRLGLAAALVHDPEV
                     LLLDEPTDGVDPVGRREIRDLLRGEAARGRAILLNSHLLSEIELTCDRVAVLRRGKVA
                     AEGRIADLTAQGSRYKMTASPVDEALLAAFRESGASVERVNGHLVVGTRDVAHLNALV
                     DALRARGGMLSELAPVRSTLEDVFVDLVRTGEAGENVQ"
     gene            complement(1642..1866)
                     /locus_tag="VF958_13770"
     CDS             complement(1642..1866)
                     /locus_tag="VF958_13770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9150223.1"
                     /translation="MPAVSGLEQEFPGKVTAENVDATKPESAAAVKELGFQSHGLVIR
                     SADGKVLWKQPDHTVKIEDAKAELKRLTSS"
     gene            complement(1885..2013)
                     /locus_tag="VF958_13775"
     CDS             complement(1885..2013)
                     /locus_tag="VF958_13775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9150224.1"
                     /translation="MRRTASAVVLAAAALAVVLVAVECARPARSEPLRVTYYYLPG"
     gene            2078..2380
                     /locus_tag="VF958_13780"
     CDS             2078..2380
                     /locus_tag="VF958_13780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9150225.1"
                     /translation="MALRNEKEIREEIRKLREKAKSLSGFWVSELAAAIYALEWVLGK
                     REKPPPQDIGEPDSGHGKLAESISKAAEKAPPATKPARATKPAPKSPVRARPPKRR"
     gene            2436..>2925
                     /locus_tag="VF958_13785"
     CDS             2436..>2925
                     /locus_tag="VF958_13785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9150226.1"
                     /translation="MNERRARWIALALLGVAVLVAAVALVLPNVLLRSDFFRKRINKD
                     PDSGWLEYDAASSRWPGTLHVKNFRFRDRDPKAEWAMSLDEADVTYSLGDLLRRRFHV
                     TSLSGRGGVFRVRSRLTPAEAKSKTLVSRLPPIAGFSDPPLLGPPERKRPPSGKVWTI
                     QVD"
BASE COUNT          509 a         1024 c          972 g          420 t
ORIGIN      
        1 gcgcaccagg tttgcgctga actcggtcga gacggccgcc gcgatcttct cgcgcgccga
       61 gaggagcgcc gcgaagaaga agatcgcgta cgtcaccatc agcgagaccg ccgtggacga
      121 cgtcaccacg ccgacgagga acgcgaacgc cacgaggcac gcgatggtga agaagatcac
      181 gacgccgccg agcaggaagc gcgggtgcgc gacgccggtc ttccagtaaa cgatcagcca
      241 gatcgacccg atgaggtaga gaaggttcgc cgcggcgagc agcagcccgc cggagtaacg
      301 cgaaaggagg agcttcgtcc gcccgaccgg ccgcgagagg tacaggtcga tcgtgccctt
      361 ctcctggagc cggggtacga ggtgcgccgt cgcgaagagc gcgaggaaag tcccgagcat
      421 gaagaggacg cccgagaaac ccgtctcgaa tcccaggacg agctggtcca tgtcgatctt
      481 gcccccgccc aggtcgagct ccttgccgaa gagcttcgca ccggccagcg ccccgtcgac
      541 gatgtcgagg ttgacggcga gcgcaaagat catgatgaag agcgtcgaca gcgcgaagta
      601 ggcgagcagc gtccagcgcg ccgaggcttc gcggaacact tcctcgacgc ttgcggccca
      661 ggctctcatt gcacgttctc ccccgcctcg cccgtccgga cgaggtccac gaacacgtct
      721 tcgagcgtcg accgcaccgg cgcgagctcc gagagcatcc cgccgcgcgc gcgcagggcg
      781 tcgacgagcg cgttcaggtg agccacgtcg cgcgtcccga cgacgaggtg cccattcacc
      841 cgctcgaccg aggctccgct ctcccgaaaa gcggccagga gcgcttcgtc caccggtgac
      901 gccgtcatct tgtagcggga gccctgcgcc gtgagatcgg cgatgcgccc ctcggcggcg
      961 accttcccgc gccgaagtac cgcgacgcgg tcgcatgtga gctcgatttc cgacagcaag
     1021 tgcgagttca acaggatcgc gcgcccgcgc gccgcctccc cgcggaggag gtcgcggatc
     1081 tcgcgccgac cgaccgggtc gacgccgtcg gtcggctcgt cgagaagcag gacctcgggg
     1141 tcgtgcacga gcgcggccgc gagccccagg cgctgcgtca tccccttgga gaatctcttc
     1201 accttcacgt ccgcccactc cgagaggcgc acgcgcgcga gcaggtccgg gatccgcgtc
     1261 ttgagcgccg tgcgctccat ccccgacata cgtccgaaaa tcgagagcgt ctgccgcgcc
     1321 gtcaggtacc ccggaaagcg gtgcccttcc gggaggtatc cgacgcgccg gcggctctcc
     1381 ggatcggcgg ccgggattcc gaacaggcgc gcggaaccct cggtcggctg cgtcaagccg
     1441 agcaggatct tgacggcggt cgtcttgccc gccccgttcg gcccgagcag gccgaacacc
     1501 tcgccggggc ccacggagag gtcgatgccg tccagcgcgg tgacgcgccg cgtcccgaac
     1561 cgggaccggt agacctttcg caggccgtcc gcctggatga ccggagccat atccccttaa
     1621 acggagcttc cgggaggaag ttcaggagga ggtcaggcgt ttgagctcgg ccttcgcgtc
     1681 ctcgatcttc accgtgtgat ccggctgctt ccagagcact ttcccgtcgg ccgaccggat
     1741 cacgagtccg tgcgactgaa atccgagctc cttcacggcc gcggcggatt ccggcttcgt
     1801 cgcgtcgacg ttctcggcgg tgaccttgcc ggggaactcc tgctcgagcc cgctcacggc
     1861 gggcatcacg cgagcgcaga tgggtcagcc gggaaggtag tagtaggtga cgcggagcgg
     1921 ctcggatcgg gcgggacgag cgcattcgac cgcaacgaga acgacggcga gcgccgcggc
     1981 ggcgagaacg accgcgctgg cggttcttct catggtgggc ctccgggaac agcctagcac
     2041 cgcccgtgaa cgtagactct ccccgagggc gaacaagatg gcgctacgaa acgaaaagga
     2101 aattcgcgag gaaatccgaa agctccgcga gaaggcgaag agcctctcgg gcttctgggt
     2161 gtcggagctc gccgcggcga tctacgccct cgagtgggtg ctcggcaaga gagaaaaacc
     2221 gccgccgcag gacatcggcg agcccgacag cgggcacggg aagctcgccg aatcgatctc
     2281 caaagccgcg gagaaagcgc cgcccgcgac gaagcccgcc cgggcgacga agcccgcccc
     2341 gaagtcgccg gtccgggccc gaccgccgaa gcgccgctga cggagtcccg ttccgcctca
     2401 cacctgttgg cagggccctt gcggagaggc cccgggtgaa cgaacgccgg gctcgatgga
     2461 tcgcccttgc tctcctcggc gtcgccgttc tcgtcgccgc cgtcgcgctg gtcctcccga
     2521 atgttctcct ccgctccgac ttcttccgca agcgaatcaa caaggatccg gacagcggct
     2581 ggctcgaata cgatgccgcc tcttcccgct ggcccggcac ccttcacgtc aagaactttc
     2641 gattccgcga tcgcgatccg aaggccgagt gggcgatgtc gctcgacgag gcggacgtca
     2701 cgtactctct cggcgacctc ctccggcgac gtttccacgt cacgagcctg tcggggcggg
     2761 gaggcgtctt ccgcgtccgg agccgcctca ctcccgcgga agcgaaatcg aaaacgctcg
     2821 tctcccgtct gccgccgatc gcgggctttt ccgacccgcc gctgctcgga ccgccggagc
     2881 ggaagcggcc gccttccggc aaggtctgga cgatccaggt cgacc
//
LOCUS       DASWAV010000657         3262 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U2665
            k141_757455, whole genome shotgun sequence.
ACCESSION   DASWAV010000657 DASWAV010000000
VERSION     DASWAV010000657.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960316
            Sequence Read Archive: SRR10260007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 3262)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3262)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:47:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,929
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3262
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_757455"
                     /isolate="SMAG_U2665"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.36444 N 132.31889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..361
                     /locus_tag="VF958_13790"
     CDS             <1..361
                     /locus_tag="VF958_13790"
                     /inference="COORDINATES: protein motif:HMM:NF021506.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF2232 domain-containing protein"
                     /protein_id="HEX9150227.1"
                     /translation="GARSARPAPSAEGARFEDLRVPAAVAPLFVAAGAGSVLLQAGAR
                     PLAGSILLPLSALYFVAGLSIICHFARKWFRFWLLRVGLYTLVAYFPMNVGVALLGLF
                     DWYVDFRRRGAGAIEKR"
     gene            358..813
                     /gene="rplI"
                     /locus_tag="VF958_13795"
     CDS             358..813
                     /gene="rplI"
                     /locus_tag="VF958_13795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011984444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HEX9150228.1"
                     /translation="MKVILADDVRGLGHRGETVTVKPGFARNYLFPQGLAWESTTANM
                     RRLSEEKKKYDEKSLHEKTAAEEVAKRVAGLTVTVSKKAGEGDVLYGSVTPADIADAL
                     ATRGVEVDRRRIELAEPIKRLGDHTVHVRLHRDVVAELTVSVQSTTPAA"
     gene            complement(1054..2235)
                     /locus_tag="VF958_13800"
     CDS             complement(1054..2235)
                     /locus_tag="VF958_13800"
                     /inference="COORDINATES: protein motif:HMM:NF040218.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9150229.1"
                     /translation="MKRLVASLLLALAAGVSFGETPPAVPTAERTNVSNVSISAQGRI
                     ETRMTLATNAAAVPAACTPSETVFCANNGRFAVQVVFSAPNLGIENATAQATTLTSDT
                     GYFWFFSANNVELVVKVVDGRAFNNFFWVFYGALSDVAYTVTVTDTATGAIKTYTNPA
                     GTIASNADTAAFSVGPSCSYTVSAASPASFGPGGGSGTVAVTTPAGCSWNAVSNSPFL
                     SVVAPASHVGSGSITFNVATNASSSSRTGTLTVAGEAVSVAQSGSAPGQFDGSWSGST
                     SQACTPSSGSAHLCAFNFIVSNNAVVRFSVGYAGPVCGISDAFTTAALETAAPITGNS
                     FQLSTTATPSTGVSATITVNGSFSSPTSSNGSGSVRITTSSPQPVCSTTVPFSFTAAK
                     N"
     gene            complement(2336..>3262)
                     /locus_tag="VF958_13805"
     CDS             complement(2336..>3262)
                     /locus_tag="VF958_13805"
                     /inference="COORDINATES: protein motif:HMM:NF013100.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HEX9150230.1"
                     /translation="YDPRQPPYRPTNYDGKFRGIVTFRRALEYSYNVSAVRVAEMVGR
                     SHVIEAAHRLGIRQKLAPYPSLALGAFEVTLAEMTSAFSVFANQGLAFPMYLFDRITD
                     ANGDLLEQTLPDAREVASPTASFQLLQMMKGVTQRGTAASAARLKLNIAGKTGTTNDW
                     TDAWFIGMTPRYTIGVWTGNDQKTVSLGRGMDGAHAALPIWIRIVEHMKENGRIDEKE
                     DFDAPPNVVFTAVDYDTGLKATPQSPKPILETFVSGSQPTEEYDSRWQEITRLPWSLQ
                     RSFYLPKKGEGGEDGSVPAPPPTPAPRPTAIP"
BASE COUNT          510 a         1047 c         1151 g          554 t
ORIGIN      
        1 gggcgcgcgc tcggcgcgac cggccccctc agccgagggc gcccggttcg aggatctgcg
       61 cgtgcccgct gcggtggcgc cgctcttcgt agcggcgggc gcgggatcgg tgctcctcca
      121 ggcgggggcc cgcccgcttg cggggagcat cctgctgccg ctctccgcct tgtatttcgt
      181 cgccgggctc tctatcatct gccacttcgc gcggaagtgg ttccgtttct ggttactccg
      241 cgtcggtctc tacactctgg tcgcgtactt cccgatgaac gtgggggtcg cgctgctggg
      301 actgttcgac tggtacgtgg attttcggcg caggggcgcg ggagcaatcg aaaagagatg
      361 aaagtcattc tggccgacga cgttcgcggg ctcgggcacc ggggcgaaac ggtgacggtg
      421 aagccgggtt tcgcccgcaa ctacctgttc ccgcagggcc tcgcctggga gtcgaccacc
      481 gcaaacatgc gacgcctttc cgaagagaag aagaagtacg acgagaagtc gctccacgag
      541 aaaaccgcgg ccgaggaggt cgcgaagcgc gtggcgggat tgaccgtgac cgtctcgaag
      601 aaggcggggg agggcgacgt gctttacggc tccgtcacgc cggccgacat cgccgatgcc
      661 ctcgcgaccc ggggggtcga ggtcgaccgc cgccgcatcg agctcgccga acccatcaag
      721 cgcctgggcg accacacggt gcacgtccgg ctgcaccgcg acgtcgtcgc ggagctcacg
      781 gtctcggtcc agtcgacgac tcccgccgcc taggggggtc aggtatccac ttgccacatt
      841 gcggcgcaat gtggctaggc cgctctcgcc aatcgatccg cgcgtcggct cgtgaagacc
      901 tgaccccgct tgtcgagttc ttcgtcggca ccttcgtcgt gcacgaggga agcgggacgt
      961 tctcccgccg accgggagcg ccgtcaattg gacttcggaa gggggaaagg aagggccggc
     1021 gcctcggcga cgccggcccg gatgtccgat tgtctaattc ttggcagcgg tgaagctgaa
     1081 ggggaccgtc gtgctgcaga cgggctgcgg ggaggacgta gtgatacgca ccgacccgct
     1141 gccgttggac gacgtcggcg agctgaacga gccgttcacg gtgatcgtgg ccgagacccc
     1201 cgtcgagggg gtcgccgtcg tcgaaagctg gaacgagttc ccggtgatcg gcgccgcggt
     1261 ctcgagagca gcagtcgtga aggcgtcgga gatcccgcag acggggccgg cgtacccgac
     1321 cgagaaacga acgaccgcgt tattcgacac gatgaagttg aacgcgcaga ggtgcgcgga
     1381 gccggaggac ggcgtgcaag cctgggaagt cgaaccgctc cagcttccgt cgaactgtcc
     1441 gggagcgctc cccgactgcg cgaccgagac ggcctcgccg gccacggtga gagttccggt
     1501 gcgcgacgag ctggacgcgt tggtcgccac gttgaaggtg atcgagccgc tcccgacgtg
     1561 cgaagccggg gcgacgaccg agaggaaggg cgaattgctg acggcgttcc aggaacagcc
     1621 ggccggcgtc gtgaccgcga cggtccccga acctccgccc ggcccgaacg acgcggggct
     1681 cgctgcgctc acggtgtagc tgcagctcgg cccgacgctg aacgccgcgg tgtccgcgtt
     1741 gctcgcgata gtccccgcgg ggttcgtata ggtcttgatc gccccggttg ccgtgtcggt
     1801 gacggtgacg gtataggcca cgtccgacag cgcgccgtag aacacccaga agaaattgtt
     1861 gaaggcacga ccgtcgacga ccttcacgac gagctccacg ttgttcgccg agaagaacca
     1921 gaagtagccg gtgtcgctcg tcaacgtcgt cgcctgggcg gtcgcgttct cgatgcccag
     1981 gttcggggcg ctgaagacga cctggaccgc gaaccggccg ttgttcgcgc agaagacggt
     2041 ttcgctgggg gtacacgcgg ccgggacggc ggcggcgttc gtggccagcg tcatccgcgt
     2101 ctcgatccgc ccctgggccg agatggacac gttcgaaacg ttcgtccgct ccgcggttgg
     2161 taccgcgggc ggtgtctcgc cgaacgacac gccggcggcg agcgcgagga gcagcgatgc
     2221 gacgagtctt ttcatctccc tctccttcct ccttcggacg gacgcaattt cggggccgac
     2281 cgccggcgcg gagtgtcgcg cggtcagcgt cggttcgagt tcgaccctgg acttctcagg
     2341 gaatcgcggt cggccggggc gcgggggtcg gagggggagc gggcacggac ccatcctcgc
     2401 ctccttcccc cttcttcggg aggtagaacg agcgctggag cgaccagggg aggcgcgtga
     2461 tctcctgcca gcgcgagtcg tactcctccg tcggctggga tcccgagacg aacgtctcca
     2521 ggatcggctt cgggctctgg ggggtcgcct tcaggcccgt atcgtagtcg acggcggtaa
     2581 agacgacgtt cgggggcgca tcgaagtcct ccttctcatc gattcgcccg ttctccttca
     2641 tgtgctcgac gattcggatc cagatcggga gagccgcgtg cgccccgtcc atcccccggc
     2701 caaggctcac ggtcttctga tcgttgccgg tccagacgcc gatcgtgtac cgcggggtca
     2761 tcccgatgaa ccaggcgtcg gtccagtcgt tcgtcgtccc tgtcttgccg gcgatgttca
     2821 gcttgagtcg ggcggccgag gcggcggttc cgcgctgcgt gactcccttc atcatctgaa
     2881 gcagctggaa cgacgccgtg gggctcgcga cctcgcgggc gtcgggcaga gtctgctcga
     2941 gcagatcccc gttggcgtcc gtgatccggt cgaagaggta catcgggaac gcgaggccct
     3001 ggttagcgaa tacggagaac gccgaggtca tctcggccag cgtcacctcg aacgcgccga
     3061 gagccagcga gggatagggc gcgagcttct gtcggatccc gagccggtgc gcggcttcga
     3121 tgacgtggga ccgcccgacc atctcggcga cccgcacggc cgacacgttg tacgagtatt
     3181 cgagcgcccg ccggaacgtg acgatgccac ggaactttcc gtcgtagttc gtcggccggt
     3241 acgggggctg cctggggtcg ta
//
LOCUS       DASWAV010000658         6332 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U2665
            k141_797737, whole genome shotgun sequence.
ACCESSION   DASWAV010000658 DASWAV010000000
VERSION     DASWAV010000658.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960316
            Sequence Read Archive: SRR10260007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 6332)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6332)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:47:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,929
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6332
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_797737"
                     /isolate="SMAG_U2665"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.36444 N 132.31889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..572
                     /locus_tag="VF958_13810"
     CDS             <1..572
                     /locus_tag="VF958_13810"
                     /inference="COORDINATES: protein motif:HMM:NF024764.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX9150231.1"
                     /translation="NTAPDVIPILEHGNQLIEQKDFAGARAEYEKALVSVPNNPIILR
                     AIAQTYYGEKKVDMAIATLKKVVELDPNDSTALLLLANLQIEKGNLEEGKATLAKVPA
                     DAIKDPGVFTNIGILLLNKKKPEDAWTYFDQAVKLAPTDADGYYYRGMAAYQAKKKAE
                     SKADFQKYLELAPQGGNAADVKEYLKTLK"
     gene            complement(565..2070)
                     /gene="gpmI"
                     /locus_tag="VF958_13815"
     CDS             complement(565..2070)
                     /gene="gpmI"
                     /locus_tag="VF958_13815"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013907628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046537 -
                     2,3-bisphosphoglycerate-independent phosphoglycerate
                     mutase activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-independent
                     phosphoglycerate mutase"
                     /protein_id="HEX9150232.1"
                     /translation="MICDGWGEAPPSEANAISLARTPTFDRWRRDYPWVTLEASGEAV
                     GLPAGMMGNSEVGHLNLGAGRMVPQDLLRVDLALRDGSFFENPALVGAADRARDAGAA
                     LHLMGLVSDGGVHSQESHLFGLLDLAQKRRVRRVHVHAFTDGRDTPPRSALGYVERLE
                     ADLRTRGGDVATVSGRYYAMDRDGRWDRVALAWEALRFGRGHRAASAREAVEEAYARG
                     ESDEFILPTVIERDGRPLGPVADGDSVIFFNFRADRARQLTRVLTEPDFAEFPRGERP
                     SVHFVGFTEYKKEFGLPVAFPAPRLDRILAAAWADSAVPNLRLAETEKYAHVTYFFNG
                     GVEQPYPGEERLLVPSWRGATYDLHPEMSAGAITDQALRAMSTSSLGAIVLNYANADM
                     VGHTGKLAETITAVETLDRCFERLEVAARENGWLLVMTADHGNAELMVDPVTGQPHTA
                     HTTNPVPILIGQEGGPLRKGGSLRDVAPTLLALQGLPIPKEMTGRDLRELT"
     gene            complement(2114..4099)
                     /locus_tag="VF958_13820"
     CDS             complement(2114..4099)
                     /locus_tag="VF958_13820"
                     /inference="COORDINATES: protein motif:HMM:NF017964.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amylo-alpha-1,6-glucosidase"
                     /protein_id="HEX9150233.1"
                     /translation="MEEPTTTTEKEIRSPLAYSGAELVAKSLDLEWLETDGLGGFACG
                     TAAGARTRKYHGWYAPAIPPPRRRILLVSGAEEFVSAIGSPTGSTGISTQVYPDAVYP
                     DGHKSLARFSLEPFPTWLHETDEFAIERSICLLRGRSATLVRWVNRGTSRIELRVRPL
                     LAFRDIHSIQREGGFTATTKIEADTARIRARTGLPELRMRAPGARALLQPDWYRRFHY
                     SAEAERGYEADEDLWSPLVWVWELKPWEEAHLVLSTEPVYDLPARLRVLEAKRRESYP
                     RSEDALFDELARRAENFLVEPPGGDPALLAGFPWFADWGRDAVIAAQGLTLASGRFRP
                     FARVLNSVASRRHGGLLPNFYPQDGEAPDYGSIDAPLWFVLAVEWFGRARRDPGKPSP
                     LLGAVREILDSYRKGTSFGIGARTDLLLAGNAPDRALTWMDAVVDGKPVTPRRGLAVE
                     VNALWHAALKSAARLERVAGDHARARDLEADAWHVARRFNEVFWSDEDERLYDVVGDD
                     GPDRSLRPNQIFAVSLAENLLPPHRARAVYWSVRRHLLTPFGLRTLDPRDPRYRGRCA
                     GSQRERDLAYHQGTVWPWLIGAFVDAHFRVFGRTAETLRTARGWLAPLRAHVREAGVG
                     SISEIFDGDPPHAPRGCFAQAWSVAELARVVHTHFSE"
     gene            4295..6301
                     /gene="tkt"
                     /locus_tag="VF958_13825"
     CDS             4295..6301
                     /gene="tkt"
                     /locus_tag="VF958_13825"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015745186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004802 - transketolase activity [Evidence
                     IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HEX9150234.1"
                     /translation="MSTVAKSIDELCVDTIRTLAIDAVQKANSGHPGLPLGAAPMAYV
                     LWSRHLKHNPRDPGWPDRDRFVLSAGHGCMLWYCLLHLTGYDLTMDDLKAFRQWGSRT
                     PGHPEYRVTPGVEATTGPLGQGTANAVGMAMAERYLADRFNRPGHEVVNHRTYALVSD
                     GDLMEGISNEASSLAGHLGLGKLTFLYDSNHVSLDGPTSLTFTEDVGKRYEAYGWQVL
                     RVEDGNTDLDAIDRALSQADAETRRPTLILVKTTIGYGSPHKAGTSEAHGSPLGPDEV
                     VATKKALGWEHEEPFFVPGDALAHFRGAVERGNRAQGEWSGRFDTWAKAFPDLAEEWR
                     RALAGEPPPGWDADLPKWKAGEKEATRVAAGKALNAIAARVPWVFGGDADLSVSTNTK
                     LKVEADFDGQTGEGRNIHFGVREHAMAGIANGMAYHGGIRPFVATFFCFSDYMRPSVR
                     ISALSELPVIFVWTHDSIGLGEDGPTHQAVEHLMSLRAMPHFTVIRPADPNEASEAWR
                     VAIEHRDGPVGLVLSRQKLPVIDPAKYAAPAGLARGAYVLAEAGGAPRLILIATGSEV
                     SLALEAREKLEAEGIATRVVSMPSWELFERQDSGYRDRVLPAAVTARLSIEAGSKLGW
                     KRWVGDRGDSVGLERFGASAPGEVVMKELGFSVENVVAKAKALL"
BASE COUNT         1053 a         2146 c         2201 g          932 t
ORIGIN      
        1 agaacaccgc gcccgacgtg atcccgatcc tcgagcacgg aaaccagctc atcgaacaga
       61 aggacttcgc cggcgcgcga gcggagtacg agaaggcgct cgtctcggtg ccgaacaatc
      121 cgatcatcct gcgcgcgatc gcgcagacct actacggaga gaagaaggtc gacatggcga
      181 tcgcgacgct caagaaggtc gtggagctcg atccgaacga ctccacggcg cttcttcttc
      241 tcgcaaacct ccagatcgag aaggggaatc tcgaagaagg aaaggcgacg ctcgcgaagg
      301 tgcctgccga tgcgatcaag gaccccgggg tgttcacgaa tatcgggatc ctgctcctga
      361 acaagaagaa gcccgaggac gcctggacct acttcgatca ggcagtgaag ctcgccccga
      421 cggacgcgga cggctactac taccgcggaa tggcggcgta tcaggcgaag aagaaggcgg
      481 agtcgaaggc ggacttccag aaatatctcg agctcgcgcc gcagggcggg aacgcggccg
      541 acgtcaagga atacctgaag accctcaagt gagctcgcgc aggtcccgcc ccgtcatctc
      601 tttcgggatc gggagcccct ggagggccag gagcgtcgga gcgacgtccc gcagagaacc
      661 ccccttccgc agcggcccgc cctcctgccc gatcaagatc ggcaccgggt tcgtcgtgtg
      721 ggccgtgtgc ggctggccgg tcacgggatc gaccatcaac tccgcgttgc cgtggtccgc
      781 ggtcatgacc agcagccatc cgttctctcg cgccgcgacc tccaggcgct cgaaacaccg
      841 gtcgagcgtc tccactgcgg tgatggtctc cgcgagcttg ccggtgtggc cgaccatgtc
      901 cgcgttcgcg tagttcagga cgatcgcgcc gagcgagctg gtggacatcg cgcgcagcgc
      961 ctgatccgtg atcgcgcccg cgctcatctc cgggtggagg tcgtacgtcg cccccctcca
     1021 cgaggggacg aggaggcgct cctcgcccgg gtagggctgc tcgacgccgc cgttgaagaa
     1081 gtaggtaacg tgagcgtact tctcggtctc cgccaggcgg agattgggga ctgccgaatc
     1141 ggcccacgcc gcggcgagaa ttcgatcgag ccgcggggcg gggaaggcaa ccggcaaacc
     1201 gaactccttc ttgtactcgg tgaaacccac gaagtggacc gacggccgtt cgccgcgcgg
     1261 aaactccgcg aagtccggct ccgtcagaac ccgtgtcagc tggcgcgcgc ggtcggcgcg
     1321 gaagttgaag aagatgacgg agtcgccgtc ggccaccggg ccgagggggc ggccgtctct
     1381 ctcgatcacc gtggggagga tgaactcgtc cgactcgcct cgcgcgtacg cctcctcgac
     1441 ggcctcgcgg gcgctcgccg cgcggtggcc gcgaccgaag cgcaaagcct cccaggcgag
     1501 cgccacgcgg tcccaccgtc cgtcgcggtc catcgcgtag taacgaccgg acacggtcgc
     1561 gacgtcgccg ccgcgtgtcc ggagatccgc ctcgagcctt tccacgtagc cgagggcgga
     1621 gcggggcggt gtgtcacgcc cgtcggtgaa agcgtggacg tggaccctcc gcacgcgccg
     1681 cttctgcgcg aggtcgagga gcccgaagag gtggctctcc tgggagtgca cgcctccgtc
     1741 cgacacgaga cccatgaggt ggagcgccgc gccggcgtca cgcgcgcggt cggcggcgcc
     1801 gacgagagcc gggttctcga agaaggagcc gtcgcgaagc gcgaggtcga cgcgcagcag
     1861 atcctgcggg accatgcgtc cggcgccgag gttcaggtga ccgacctccg agttgcccat
     1921 catccccgcg ggaagcccga cggcttcgcc ggacgcctcg agcgtcaccc aggggtagtc
     1981 cctccgccag cggtcgaagg tcggagtgcg cgcgagcgag atggcgtttg cctccgacgg
     2041 cggcgcctct ccccagccgt cgcagatcac gaggaggatc cttctctttg cggaggtcaa
     2101 atcgccacgc cgctcactcg gaaaagtgcg tgtggacgac ccgggcgagc tcggcgacgc
     2161 tccacgcctg ggcgaagcat ccccgcggcg catgcggcgg atcgccgtcg aagatttccg
     2221 agatcgagcc cacccccgcc tcccgcacgt gcgcgcgtag gggcgcgagc cagccgcgcg
     2281 ccgtgcggag cgtttcggcg gtccggccga agacgcggaa gtgcgcgtcg acgaacgcgc
     2341 cgatcaacca cggccacacg gtcccctggt ggtacgcgag gtcgcgttct ctctgcgacc
     2401 ccgcgcagcg accgcggtag cgcgggtccc gcgggtcgag cgtgcgcagg ccgaaaggcg
     2461 tcagcaggtg ccgccggacc gaccagtaga ctgctcgggc gcggtgcggc ggaagcaggt
     2521 tctccgcgag cgacaccgcg aagatctggt tcggacggag gctgcggtcg ggtccgtcgt
     2581 cgccgaccac gtcgtacagc cgctcgtcct cgtccgacca gaagacctcg ttgaaacggc
     2641 gtgcaacgtg ccacgcgtcc gcttcgaggt cgcgcgcgcg cgcatggtct ccagcgacgc
     2701 gctcgagacg cgcggcggac ttgagcgccg cgtgccagag ggcgttcacc tcgacggcga
     2761 ggccacgcct cggcgtcacg ggtttcccgt cgacgacggc gtccatccac gtcagcgccc
     2821 ggtccggagc gttgccggcc agcagcaggt ccgtccgggc gccgattccg aaggacgtcc
     2881 cctttcggta ggagtcgagg atttcgcgaa cagcgccgag gagcggcgag ggcttgccgg
     2941 ggtcgcgccg cgcccttccg aaccactcga cagccagaac gaaccagagc ggcgcgtcga
     3001 tcgaaccgta gtccggcgcc tcgccatcct gcggatagaa gttcggaaga aggcctccgt
     3061 gccgcctcga tgcgacggag ttgaggactc gggcaaaggg ccggaagcgg ccgctcgcca
     3121 gggtcaggcc ctgtgcggcg atgacggcgt cgcgacccca gtcggcgaac cacggaaatc
     3181 ccgccagcag cgccggatct ccgccagggg gctcgaccag gaagttttcc gcacgacgtg
     3241 cgagctcgtc gaacaaggcg tcttcgctgc gagggtagga ctctcgtcgc ttcgcctcca
     3301 ggacccggag ccgcgccggc aagtcgtaaa ccggctccgt cgaaagcacc aggtgcgctt
     3361 cctcccacgg cttgagctcc cagacccaga cgagcgggct ccagaggtct tcgtccgcct
     3421 cgtaacctcg ctccgcctcg gcggagtagt gaaagcggcg gtaccagtcc ggctgcagca
     3481 gcgcgcgcgc gcccggcgcc cgcattcgca gttccggcaa tccggtccgc gctcgaatgc
     3541 gggccgtgtc cgcctcgatc ttcgtggtcg cggtaaagcc tccctcgcgc tggatcgaat
     3601 ggatgtcgcg gaaggcgagc agagggcgca cgcggagctc gatccggctc gtcccgcgat
     3661 tgacccaccg aacgagcgtc gccgatcggc ctcgaagcag gcagatcgac cgctcgatcg
     3721 cgaactcgtc ggtctcgtgc agccacgtcg gaaagggctc cagcgagaag cgcgcgagac
     3781 tcttgtgtcc gtccgggtac acggcgtcgg gatagacctg cgtcgaaatg ccggtcgagc
     3841 ctgtcggaga tccgatcgcc gacacgaact cctcggcacc cgagacgagg agaatacggc
     3901 gacgcggcgg agggatcgcg ggcgcatacc agccgtgata cttgcgggtc cgcgcacccg
     3961 cggcggttcc acacgcgaac cccccgagac catcggtctc gagccattcg aggtcgagcg
     4021 actttgccac gagctccgcg cccgagtacg cgagcggact ccggatctcc ttctcggtcg
     4081 tggtcgtcgg ttcttccatc ggctccgggg cgcgctctga ggcgagtgtc ccggtccgat
     4141 tattcctcat tggatgcgac gtcggaacgc gcgggaaatt gcccttttcg cgcgaccgcc
     4201 gacgcaggat cgggtggact aagattgggc gctgccgtct tcacgggcgc ggttcttgcg
     4261 acgtaggtat gcggtgtctc gacgggaggt cttcttgagc acggtcgcaa agtcgatcga
     4321 cgagctctgc gtcgacacga tccgcacgct cgcgatcgac gccgtgcaga aggcgaactc
     4381 cgggcacccg ggcctgccgc ttggcgccgc gccgatggcc tacgttctct ggtcgcgaca
     4441 cctgaaacac aacccgcgcg atcccggctg gcccgaccgc gatcgcttcg tcctttccgc
     4501 cggccacggt tgcatgctct ggtactgcct gctccacctg actggatacg acctgacgat
     4561 ggacgacctc aaggcgttcc ggcagtgggg gagccgcacg cccggtcatc ccgagtaccg
     4621 ggtgacgccg ggggtcgagg ccacgacggg cccgctcggc cagggcaccg cgaacgcggt
     4681 cggaatggcg atggcggagc ggtacctcgc cgaccgattc aaccggcccg gacacgaggt
     4741 cgtgaaccac cggacgtacg cgctcgtttc ggacggcgat ctgatggaag gaatttccaa
     4801 cgaggcctcg tcgctcgccg gacacctcgg cctcgggaag ctcacgttcc tctacgactc
     4861 gaaccacgtc tcgctcgacg ggccgacgtc gctgacgttc accgaggacg tcggcaaacg
     4921 ctacgaagcc tatggctggc aggtcctgcg cgttgaggac gggaacaccg acctggacgc
     4981 gatcgaccgc gcgctgtcgc aggcggacgc ggagacgcgg cggcccacgc tcatcctggt
     5041 gaagacgacg atcggctacg gctcgcccca caaggccggc acgtcggagg cgcacggcag
     5101 cccgctcggc cccgatgagg tcgtcgcgac gaagaaggcg ctgggctggg agcacgagga
     5161 gccgttcttc gtgcccggcg acgcgctcgc gcactttcgc ggcgccgtcg agcgcgggaa
     5221 ccgcgcgcaa ggcgagtgga gcggccggtt cgacacatgg gcgaaggcgt tccccgacct
     5281 cgcggaggag tggcggcgcg ccctcgccgg cgagccgccg cccgggtggg acgccgatct
     5341 gccgaagtgg aaggcgggcg agaaggaggc cacgcgcgtc gccgcgggca aggctctgaa
     5401 cgcgatcgcg gcgagggtac cgtgggtctt cggcggcgac gccgacctct cggtgtccac
     5461 gaacacgaag ctcaaggtcg aggccgactt cgacggccag accggagaag gacgcaacat
     5521 ccacttcggc gtgcgcgaac acgcgatggc cgggatcgcg aatggaatgg cgtaccacgg
     5581 cggcatccgg ccgttcgtcg cgacgttctt ctgcttctcg gattacatga ggccctcggt
     5641 gcggatctcg gccctctccg agctgccggt gatcttcgtc tggacccatg actcgatcgg
     5701 actcggcgag gacggcccga cgcaccaggc cgtcgagcac ctgatgtcac tgcgggcgat
     5761 gccgcacttc actgtcattc gccccgccga tcccaacgag gcgtccgagg cgtggcgcgt
     5821 cgcgatcgag caccgagatg ggccggtcgg gctcgtgctc tctcgccaga agctgccggt
     5881 gatcgacccc gcgaaatacg cggcgccggc cgggctcgcg cgcggggcgt acgtgctcgc
     5941 ggaagcgggc ggcgcgccgc ggctgatcct gatcgcgacc ggcagcgagg tttcgctcgc
     6001 cctcgaagcg cgcgaaaagc tcgaggccga gggcatcgcg acgcgcgtgg tctcgatgcc
     6061 ctcgtgggag ctcttcgaga ggcaggactc agggtatcgc gatcgggtgc tgccggcggc
     6121 cgtcactgcc cgtctctcca tcgaggccgg atccaagctc ggctggaagc gctgggtggg
     6181 ggaccggggt gactcggtcg gcctcgagcg gttcggcgcc tccgccccgg gagaggtcgt
     6241 gatgaaggaa ctcggcttca gcgtcgaaaa cgtagtggcg aaggcaaagg ccctgctgtg
     6301 aagatcgccg tcgggtgcga tcacgcgggc tt
//
LOCUS       DASWAV010000659         3335 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U2665
            k141_677852, whole genome shotgun sequence.
ACCESSION   DASWAV010000659 DASWAV010000000
VERSION     DASWAV010000659.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960316
            Sequence Read Archive: SRR10260007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 3335)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3335)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:47:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,929
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3335
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_677852"
                     /isolate="SMAG_U2665"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.36444 N 132.31889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..379
                     /locus_tag="VF958_13830"
     CDS             <1..379
                     /locus_tag="VF958_13830"
                     /EC_number="4.1.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006980626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="phosphomethylpyrimidine synthase ThiC"
                     /protein_id="HEX9150235.1"
                     /translation="VKDGVIAYKIAAHAADLAKGHPGARLWDDTLSKARFEFRWEDQF
                     HLALDPETAQEFHDETLPAEGAKLAHFCSMCGPHFCSMKITEEVRAYATSHGVAEEKA
                     LEAGLEEKAEEFVAGGGEIYRKS"
     gene            407..1201
                     /locus_tag="VF958_13835"
     CDS             407..1201
                     /locus_tag="VF958_13835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9150236.1"
                     /translation="MRTSIRTACSISLMIWSLAATAWALPERDVLVIAGAGVSQAWNT
                     EFDLANPGPTPLTVEIGRLDVPLSEGQCPGIDPCIVAVDHRVIPPNGTIHTNAYSIGA
                     GLVSTFYVVSPDAGGSLPVVHARTVDRNRPARGTDIPVVRRSTLIASGENGLSFPAAV
                     RSASVHSNLVLAEISAKADLEVLVEVFSAEGERLAGQNFHVGRDNALGRDGTLFLVDI
                     LGMLGVSELDGGQIRVTKIGGTGLMWGLLANIYDEGRVSVSVGANP"
     gene            1198..2817
                     /locus_tag="VF958_13840"
     CDS             1198..2817
                     /locus_tag="VF958_13840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9150237.1"
                     /translation="MTRALALLLAFFVPSGLFAARGVFRDFPLPSAGAEPTSVIRLRG
                     ETWFTEFAANKIGRISYRGELTEYTIPTPASGPLGIAADFENIWFTEFRANKIGRITP
                     EGAIAEFSIPTSASGPYAITGEVTDSALWFTELEGNKLGRISTDGVITEFVIPTLNSG
                     PMGISRGPWGTDIWFTESRANKIGWMFREGGTVTEIPIPTPGSGPAGITMDSGGRIWF
                     TESDADRIGRITRPVARPVIEEFALPGRESGPLAIDTREDGTVWFTERFTNKIGRIDV
                     AGRIVEYPIATPASGPFGIAAEGWYVWFAERGSGRISRMIRDAIIAVGVGVSGDWDTE
                     FRLANANFEADDVYIGLWPEAQTVCPGSCFNQTGIRLTGSGTATRRASQFLFGLMSLH
                     LRGELEEAPPTAKARVFNSARPTQGIEVPLIRLSTLWERNPSVLAFPSARRGADVHSN
                     LVLSEVGWDDDVTVLVEAFSADGTSRGSALHRIPPGATLFLTDVLAQLGVSEFDGQIR
                     VTRISGTGLMWGLLATVSDDGGVAVSPGANP"
     gene            2862..>3335
                     /locus_tag="VF958_13845"
     CDS             2862..>3335
                     /locus_tag="VF958_13845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9150238.1"
                     /translation="MRGARVVLLAVLFPCTVGLSQTATRPTASAAFSGSLVLPADEHT
                     ETAVGGSVELDFPLSTSFSAGVVAGHWSGKSDLDKDSTETYLLGVVTHRWGSGRWRPF
                     LHAGGGFYILKFQFQSRSRFSPSETETVGGGFAGPGVDCVLSPNSALELRARYHLV"
BASE COUNT          575 a         1067 c         1075 g          618 t
ORIGIN      
        1 cgtcaaggac ggcgtcatcg cctacaagat cgcggcgcac gcggcggatc tcgcaaaggg
       61 ccatccgggg gcgcggctgt gggacgacac gctgtcgaag gcgcggttcg agttccgctg
      121 ggaggaccag ttccacctcg ctctggaccc cgagaccgcg caggagttcc acgacgagac
      181 tctgccggca gagggcgcga agctcgccca cttctgctcg atgtgcggcc cgcacttctg
      241 ctcgatgaag atcaccgagg aagtgcgcgc gtatgccacg tcccatgggg tcgccgagga
      301 gaaggcgctc gaggcgggac tcgaagagaa ggccgaggaa ttcgtcgccg gcggcggaga
      361 gatctaccgg aagagctgaa cgccggtcgg gaagcaggca aaggaggtgc gcacgtccat
      421 tcgcaccgcg tgctcgatct ctctgatgat ctggagtctc gcggccaccg catgggcatt
      481 gccggagcgt gacgtcctgg tgatcgccgg ggccggggta tcgcaggcgt ggaatacgga
      541 atttgatctc gcgaacccgg gcccgactcc tctcaccgtc gagatcggcc gtcttgatgt
      601 ccctctgtcc gaggggcagt gccccgggat cgacccctgc atcgtcgccg tcgaccaccg
      661 ggtcatcccg ccgaacggca ctatccatac gaatgcctac tcgatcggcg cggggctcgt
      721 ctcgacgttc tacgtcgtct ccccggatgc cggtggctcg ctgccggtcg tgcacgcgag
      781 gacggtcgac cgcaatcgcc cggctcgcgg caccgacatc cctgtcgttc gtcgctcgac
      841 tctcatcgca tccggagaaa acgggctctc gttccctgcg gccgtgcgaa gcgcgagcgt
      901 gcacagcaat ctagttctcg cggagatctc ggcgaaggcc gatttagaag ttctggtgga
      961 agttttctcg gcggaaggcg aacgactggc cggccagaat tttcacgtcg ggcgcgacaa
     1021 cgctctgggg agggacggga cgcttttcct cgtcgacatc cttgggatgc tcggtgtttc
     1081 cgagctcgac ggcggacaga tccgcgtcac gaagataggc ggaaccggct tgatgtgggg
     1141 cctcctcgcg aacatctacg acgaaggccg cgtctccgtc agcgtcggag cgaatccatg
     1201 acccgggccc tggcgcttct cctggccttt ttcgtcccga gcgggctgtt cgcggcacgc
     1261 ggggttttcc gggactttcc tcttccgtcc gcaggcgccg agcccacgtc ggtcattcgt
     1321 cttcggggcg agacttggtt cacggagttt gcagcgaaca agatcggacg catctcctat
     1381 cggggtgagt tgaccgagta caccatcccc acgcccgcga gcggtccgct cggcatcgcg
     1441 gccgacttcg agaacatctg gttcacggag ttccgcgcca acaagatcgg ccgcatcact
     1501 ccggagggag caatcgccga gttctcgatc ccaacttccg cgagcggtcc gtacgcaatc
     1561 acgggggagg tcaccgactc ggcactctgg ttcacggaac tcgaaggcaa caagctcggc
     1621 cgtatttcga cggacggtgt catcaccgag ttcgtgatcc ctacgctgaa ctccggcccg
     1681 atgggaatca gccgggggcc ctggggaacc gacatctggt tcaccgagtc gcgcgcgaac
     1741 aagatcggat ggatgttccg cgaaggcggt accgttacgg aaatcccgat accgactccg
     1801 ggcagcgggc ccgccggcat caccatggac tccggagggc gaatctggtt cacggaatcc
     1861 gacgcagatc gcatcggaag aatcacccgg cctgttgcgc gtccagtcat cgaggagttt
     1921 gccttgccgg gtcgagaaag cggtcccctg gcgatcgaca cgcgggaaga cggcacggtc
     1981 tggttcacag aacgcttcac gaacaaaatc ggaaggatcg acgtcgccgg aaggatcgtc
     2041 gagtatccga ttgcgacgcc cgcgagcggg cccttcggga tcgcggccga gggttggtac
     2101 gtctggtttg ccgaacgcgg atccggaagg atctcccgca tgatccgcga cgccatcatc
     2161 gccgtcggag tgggagtctc aggagactgg gacaccgagt tccgactggc gaatgcgaac
     2221 ttcgaggcgg atgacgtcta catcggtctt tggccggaag cccagacggt ctgccccggc
     2281 agttgcttca accagaccgg gattcgcctg accgggagcg gtactgcgac gcgtcgagcg
     2341 tcacaattcc ttttcggtct gatgtctctt cacttgcggg gcgagctcga agaagcccct
     2401 ccgaccgcga aggcgcgagt cttcaactct gcgcgcccca cccaggggat cgaggttcct
     2461 ctcatccgcc tctcgacgct ctgggagcgc aatccttcgg tgctggcatt tccttcggcg
     2521 agaagaggag cggacgtgca cagcaacctc gtcttgagcg aagtcggttg ggatgacgac
     2581 gtgaccgttc tcgtcgaggc cttttccgca gacggaacct cccgcgggag tgctctgcat
     2641 cggataccgc ctggcgcgac cctgtttctc accgatgttc tcgctcagct gggagtctcg
     2701 gagttcgacg ggcagattcg agtcacgcga atcagcggga ccggcctgat gtggggcctc
     2761 ctcgcgacgg tctctgacga cgggggagta gccgtcagtc ctggagcaaa cccgtagctc
     2821 tccaaacgga gtcgttcgcg cgaccgtaaa ccgggtactt catgagaggc gcacgagtcg
     2881 tccttctcgc cgtcctgttc ccttgcaccg tcggtctgtc tcagacggcg acccgtccca
     2941 cggcctcggc cgccttttcc gggtcgctcg tcctgccggc cgacgagcac actgagacgg
     3001 ccgtcggcgg atccgtcgag ctcgacttcc ccctctcgac gtctttttcg gcgggagtgg
     3061 tagccggaca ttggagcggc aagtccgacc tcgacaagga ttcgacggag acctatctcc
     3121 tcggagtcgt gacgcaccga tggggctccg gccggtggcg gccgttcctc cacgccggcg
     3181 gcggcttcta catcctcaag ttccagtttc aatcccggag ccggttctct ccgagcgaaa
     3241 ccgagaccgt cggcggaggg ttcgcgggcc cgggagtcga ctgcgttctc tctcccaact
     3301 cggcattgga gctgagagcc cgatatcacc tcgtg
//
LOCUS       DASWAV010000660        11681 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U2665
            k141_797856, whole genome shotgun sequence.
ACCESSION   DASWAV010000660 DASWAV010000000
VERSION     DASWAV010000660.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960316
            Sequence Read Archive: SRR10260007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 11681)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11681)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:47:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,929
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11681
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_797856"
                     /isolate="SMAG_U2665"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.36444 N 132.31889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..696)
                     /locus_tag="VF958_13850"
     CDS             complement(<1..696)
                     /locus_tag="VF958_13850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014392940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M28 family protein"
                     /protein_id="HEX9150239.1"
                     /translation="MKRSITTIVIASTLLVPTTRAASPGPALPEQVRRTAEALRERAF
                     TGTRAAEWVRSLIDTAGARLSGSPGDAAAIAWGLATLKTLGFANVRAEKVMAPFWVRG
                     EETGEVTAPSRHKLFLTALGGSVPTPEAGIEAEIVEVASLEELAAKKDSDVRGKVVFF
                     NRRMRRASDGAGYGEAVKVRGGGPSAAAKLGAAGVLIRSIGTDSNRTPHTGSLRYEEG
                     IARIPAAAVSNPDA"
     gene            806..4426
                     /locus_tag="VF958_13855"
     CDS             806..4426
                     /locus_tag="VF958_13855"
                     /EC_number="4.1.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020544135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004591 - oxoglutarate dehydrogenase
                     (succinyl-transferring) activity [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional oxoglutarate
                     decarboxylase/oxoglutarate dehydrogenase thiamine
                     pyrophosphate-binding subunit/dihydrolipoyllysine-residue
                     succinyltransferase subunit"
                     /protein_id="HEX9150240.1"
                     /translation="MAPGKTADLAEDLVAEFGDNATYVADLLARYRQSPDSVDAEWRQ
                     FFRERLGEAVTSPARPTEPTSPPPPLAPSVPPEVAASSVRAGEGEPLSGGALRIAENM
                     EASLAVPTATSQRQIPIKLADENRRLINEYRAGNDQPKISFTQLIAWAIVRALETFPR
                     LNDAYDDSSGTPARVRRDQIRFGLAVDVTKSDGSRTLLVPNVKGVEKMGFREFADAAD
                     DIVRRARTAKLQVSDFEGTTVSLTNPGTLGTTASVPRLMPGQGLIVATGAIGYPAEFS
                     AVAPETISQLAVSKVVTFTSTYDHRVIQGAESGAFLARIEELLLGKDGFYESVFADLG
                     IPHKPYRWAVDRNPPFGRRDEIEKQARVLELINAYRVRGHLIADVDPLRMVPVQQHPE
                     LDLETYGLTIWDLDREFWTGGLAGGEHMPLSEIIGVMRRVYCGKVGVEYRFISNPVEK
                     EWVRNHVGAPPQALPPEVRKRIFEKLLAAETFERFLGTKYLGQRRYSIEGCETVIALL
                     DQLMEGAGERGIEEIAMGVTHRGRLNLLANVVGNSAERIFASFEGTVHPDFPADEGDV
                     KYHQGAQGAHKTASGREVAITVPSNPSHLEAVDPVVEGMVRAKQDRHGHGPEAWKHAL
                     AVIVHGDAAFAGQGMVAEVFNLAQLRGFRTGGSIHLIINNQIGFTTPPASGRSSLYST
                     DVAKINQVPIFHVNGDAPEAAYRVLQIALDYRQEWHKDVVLDLIGFRRHGHQETDEPT
                     YTQPLMYRRIQEHPGVRALYAQKLVKDGVLTEVQVAEMEERQKAQYEEALAAAREIAA
                     RPTAASEPSPIPDSRSPRSDVETGVARESLARIGRVLTTVPPGFHINPKMVSQLARRA
                     KMAEGSEPLDWSTAEALAFGSLLLEGTPIRLTGQDTSRGTFSQRHIVFHDTATGDTWT
                     PLCDLDPARPKLAVYDSPLSEVSVLGFEYGYSVEAPETLDLWEAQYGDFANGAQVVLD
                     QFVTSAEDKWQQTSRLGLLLPHGYEGQGPEHSSARIERYLQLCAEDNIQVCNVTTPSQ
                     YFHLLRRQMRQEAAKPLVIFTPKSLLRFPASFSPLEDLTRGSFLSVVDDPEVADRAAV
                     ARVLLCSGKIFYDLHAAREERKTNAVAIVRLEQLYPFAADALRAALGRYVSARDFVWV
                     QEEPQNMGAWSFVRPRRSEFLPTGAALWYAGRAPSASPATGSAAVHKRELEQLIAEAF
                     GR"
     gene            complement(4480..5346)
                     /locus_tag="VF958_13860"
     CDS             complement(4480..5346)
                     /locus_tag="VF958_13860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018127992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX9150241.1"
                     /translation="MFDTKILAERSILVTGGGSGLGLAMAKKFAAHGAKVTIAGRTLD
                     RLERAAGEIRAVASAGGEADFHPADVREPAEVEALVAHAIARFGKIDSLVNNAAGNFL
                     VTSEDLTPNGFDAVVRTVLHGSVYCTLAVGRHLLERKAPGSIVSVVTTYAWTGTGFAL
                     PSACAKAGVLAMTRSLAVEWGHAGIRLNAVAPGPIPTEGAWSRLMAFPEAEKKTLERI
                     PLGRFGTADELANLVTFLLSDLCPYQTGDCVTMDGGEWMAGAGEFSDYRKIPREVFKE
                     ALERMRPRKRKP"
     gene            5425..6027
                     /locus_tag="VF958_13865"
     CDS             5425..6027
                     /locus_tag="VF958_13865"
                     /inference="COORDINATES: protein motif:HMM:NF012976.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocation pathway signal
                     protein"
                     /protein_id="HEX9150242.1"
                     /translation="MANENVSRRSLLARVVALPAALALASRPARPEEACAATEADFQG
                     PMYLAGAPRRSVLAGPKEPGERLTIRGTVFGSDCRTPLPRTLLDVWQADAQGEYHWKD
                     EDYRLRGQLLTNDRGEYEIATIKPAGYGGRPAHIHFTIAAPGHASLTTQLYFRGDPRL
                     DHDVCGAACNSDDPHRIVELAREPRGLVGRFDVVLKSARA"
     gene            complement(6024..6491)
                     /locus_tag="VF958_13870"
     CDS             complement(6024..6491)
                     /locus_tag="VF958_13870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9150243.1"
                     /translation="MVRRVLERGRAIEPQVSIVFEALVLCDDVRFENNGKLLLIGVYS
                     DVVQVVKLPLQMRSIGLAIKAKVLETGKFPFSVSVSDPQGNKILDANGELNYEGEAGR
                     TIWFPVVMGPALLTIEGTYAVRISLGDGPYVEEDFLVRKTPVPEVRVTEKKPN"
     gene            complement(6455..8425)
                     /locus_tag="VF958_13875"
     CDS             complement(6455..8425)
                     /locus_tag="VF958_13875"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013866.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HEX9150244.1"
                     /translation="MKKILPALALAAWTISAFATPAGRPSIESVLATLQSVQDYDAAT
                     ISPDGKRVAWVRKIKDARGAWKLAAIEVSDLTAPSRKSRRVTAGAGGRAHDERGPAWS
                     PDSTRIAFLSDAAKEKQMQVWVAPAEGGPPRRTTSVKGQLSDLRWSPDGHSIAFLFVE
                     GSAQETGALTAYKPDAGVVAETIEEQRIAVLDADTGKVRSISPANLYVYDYDWSPDGK
                     RFAAEAAEGSGTNNYWIAQLYTVDAASGETKSLWKPPLQIACPRWSPDGKEIAVIHGI
                     MSDEGLTGGDIWTVPVAGGPARNRTEGIAASASAIFWRSPGEILFTEYVDGDEGVAAL
                     DASGNVRTLWRGPAVVKRFSVAREGSASAAIRSSFSQPPEIVAGPIGTWAPLTRGNAG
                     VRPFWGEARSLTWQSDGQRVQGWLLSPLDRDESKKSPMVVVVHGGPSGSSTASWPSRW
                     TAILPSQGYYVFLPNPRGSFGQGEAFTRGNVKDFGYGDFRDIDRGVDAALAAAPIDPK
                     RLGICGWSYGGYMTMWAVTQTERYAAAVAGAGIVSWQSYYGQNRIDQWLLPFFGASVY
                     DDPEVYARSSPITFIKKAKTPTLVLHGDRDSEVPTPQGYEFWHALKTLGVPTTLVIYE
                     NEGHAIEDPKHQLDRDTRLVKWFDEYLKGGAR"
     gene            complement(8463..9932)
                     /locus_tag="VF958_13880"
     CDS             complement(8463..9932)
                     /locus_tag="VF958_13880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012566643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HEX9150245.1"
                     /translation="MAAETVPGLSLKTDLFIGGEFVPSASGKRFSTTNPATGERIAEV
                     AEAGKEDLDRAVDAARDAFESGPWAAMKPRERGRILIKAADLLLSRAEEFGRVETLDN
                     GKPIFESAKIDMPVAAEALSYFGEYADKLYGDTFPARSDAFLMTMREPVGVVGAITPW
                     NFPLLQAMWKIAPALALGNSVVLKPASLTPLTALLFADLLREAGLPPGAFNVVPGPGA
                     QVGAAMAEHPGIDKISFTGETQTGKAILRAAAGTVKRVSMELGGKSPNVVFADADLDA
                     AAKGAINAIFYGKGELCSAGSRLLVEEGAHDALVEKVVDRAKKMIPADPLHPKTRLGS
                     LVSEKQRENVERYVESGKREGAKLVAGGNRAAVDGKGAFFEATVFDGVRPEMKIAREE
                     IFGPVLATLTFQDEADAVAIGNSTVYGLAAAVWTKDVKKAFRTAKALKAGTVWVNTYN
                     YYDPGLPFGGYKESGFGRERGHYALEEYTQVKSVWVDLT"
     gene            complement(9933..10769)
                     /locus_tag="VF958_13885"
     CDS             complement(9933..10769)
                     /locus_tag="VF958_13885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007908087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HEX9150246.1"
                     /translation="MSTPVQTRDWTSPEVGSEIVHYRADSGIAIVEMDDPPANTYTYA
                     MMRQLDDAILKARFDDDVHVIVLRGKGDRFFSAGANIGMLNAVTPGFKYFFCLHANET
                     LLRLEHTPKLVIAALNGHTVGGGLEIAMAADIRIARKDAGKIGLPEVTLGVLPGTGGT
                     ARLGKLIGKSRAIELMITGNTFSFEEAADLGLVNQVIDATPAEWWEQVMDYARQFVPP
                     NKASKAVGRIKRSVQSGWDLPLESALAVERELQQLLFQSDDAKEGIAANQEKRVPKFK
                     GR"
     gene            complement(10803..11213)
                     /locus_tag="VF958_13890"
     CDS             complement(10803..11213)
                     /locus_tag="VF958_13890"
                     /inference="COORDINATES: protein motif:HMM:NF014448.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor biosynthesis protein MoaE"
                     /protein_id="HEX9150247.1"
                     /translation="MFLSSEPIDLAAFLGEARPSDGAVCVFVGVVRNESHGRQSVAVE
                     YQAYGAMAESEMARIADSLTTEWPGVFLRIRHRVGRLAVGEPSVAIVATAPHREEAFA
                     ACREAIERVKKTVPIWKREIRPDGSSEWVDPTRG"
     gene            complement(11221..11466)
                     /gene="moaD"
                     /locus_tag="VF958_13895"
     CDS             complement(11221..11466)
                     /gene="moaD"
                     /locus_tag="VF958_13895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018141219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin converting factor subunit 1"
                     /protein_id="HEX9150248.1"
                     /translation="MEVRVLYFASFRDAAGRDEEARELPSGCRVGELWQELARDVPGF
                     SRFLSMPAAAINREYASAEAELHDGDEVAFLPPVAGG"
BASE COUNT         1874 a         4055 c         3871 g         1881 t
ORIGIN      
        1 cgcgtcggga ttggaaaccg cagccgccgg gatcctcgcg attccctcct cgtatcggag
       61 cgagccggtg tgcggcgtcc ggttcgagtc ggtcccgatg gaacggatga gaactcctgc
      121 ggcgccgagc ttcgccgcgg ccgagggacc tcctccgcgg acctttacgg cctcgccgta
      181 gccggcgccg tcggacgcgc gccgcatgcg gcggttgaaa aatacgacct tcccgcgcac
      241 gtcggaatcc ttcttcgccg cgagctcctc gagggacgcg acttccacga tctccgcctc
      301 gatccccgcc tccggcgtcg gcacgcttcc gccgagcgcc gtgaggaaga gcttgtgtcg
      361 ggaaggagcc gtgacctcgc ccgtctcttc gcctcggacc cagaacggcg ccataacctt
      421 ctcggcgcgc acgttcgcga acccgagcgt cttcaacgtc gcgagacccc atgcgatcgc
      481 ggccgcgtct cctggggagc cggagagacg cgccccggcg gtgtcgatca gacttcgaac
      541 ccactccgcc gcgcgcgtgc cggtgaaggc cctctcccga agagcctcgg cggtccttcg
      601 gacctgctcg ggcagggccg ggccgggact cgccgcccgg gtagttggaa cgagaagcgt
      661 cgacgcgatg acgatcgtcg tgatggagcg tttcaaggcg ggtcctccag ttcggggcag
      721 ccgaatcgta ccaagcggcg aagccatccg gaccgcgctc gcccgggaca ggctcggcgg
      781 aggatctgag aaatagaatc gggtcgtggc ccccgggaag acggcggatc tcgccgaaga
      841 cctcgtcgcg gagttcggcg acaacgcgac gtacgtcgcg gacctcctcg cgcgctaccg
      901 gcagagtccg gactctgtcg acgcggagtg gcggcagttc ttccgagagc gactggggga
      961 ggcggtcacg tccccggccc gacccacgga accgacgtct ccgccgccgc cgctggctcc
     1021 ctcggttcct cccgaggtgg ccgcctcttc cgtgcgggcg ggcgaaggtg agccgctttc
     1081 cggcggagcg ctacggatcg ccgagaacat ggaagcgtcg ctcgcggtcc cgacggcgac
     1141 ctcgcagcgg cagatcccga tcaagctcgc cgatgagaac cggcggctca tcaacgagta
     1201 ccgggccggg aacgaccagc cgaaaatctc gttcacgcag ttgatcgcgt gggcgatcgt
     1261 ccgggcgctc gagacgtttc cccggctgaa cgacgcctac gacgattcga gcggaactcc
     1321 cgcccgcgtt cggcgtgacc agatccggtt cggtctcgcg gtcgacgtca cgaagtcgga
     1381 cggctcgcgg acgctcctcg ttccgaacgt caagggcgtc gagaagatgg ggttccggga
     1441 gttcgccgat gccgcggacg acatcgtccg ccgggcgcgc accgccaagc tccaggtctc
     1501 ggacttcgag gggacgacgg tgtcgctgac gaaccccggt acgctcggaa ccaccgcgtc
     1561 ggtcccgcgg ctgatgccgg gccagggact gatcgtcgcg acgggcgcca tcggttaccc
     1621 cgcggagttc tccgcggtgg cgcccgagac gatctcgcag ctcgccgtca gcaaggtcgt
     1681 gacgttcacg tcgacgtacg accaccgcgt gatccagggc gccgagtccg gcgcgttcct
     1741 cgctcggatc gaggagctgc ttctcggcaa ggacgggttt tacgagtcgg tctttgccga
     1801 cctcggcatc ccgcacaagc cgtaccgctg ggcggtcgac cgcaaccccc ccttcggacg
     1861 tcgggacgag atcgaaaaac aggcgcgcgt gctcgaactg atcaacgcct accgcgtccg
     1921 cggacacctg atcgcggacg tcgatccgct ccgcatggtc ccggtgcagc agcaccccga
     1981 gctcgacctc gagacctacg ggctcaccat ctgggacctc gaccgggagt tctggacggg
     2041 ggggctcgca ggcggcgagc acatgccgct ctccgagatc attggcgtga tgcgccgcgt
     2101 gtactgcggc aaggtcggcg tcgagtatcg cttcatctcc aacccggtcg agaaggagtg
     2161 ggtgcgaaac catgtcggcg caccgcccca ggcgctgccg ccggaagtgc gaaagcggat
     2221 cttcgagaag ctcctcgcgg ccgagacctt cgagcgattc ctggggacga aatatctcgg
     2281 acagcgccgc tattcgatcg agggttgcga gaccgtgatc gctctcctcg atcagttgat
     2341 ggaaggcgct ggcgagcgag ggatcgagga aatcgcgatg ggagtgactc atcgcggccg
     2401 cctgaacctg ctcgcgaacg tcgttggcaa ctccgctgaa cgcatcttcg cgagcttcga
     2461 ggggacggtg cacccggatt tccccgccga cgaaggggac gtcaagtacc accagggcgc
     2521 gcagggggcc cacaagacgg cgagcggccg cgaggtcgcg attacggtgc cctcgaaccc
     2581 ttcgcacctc gaggccgtgg accccgttgt cgaaggcatg gtacgcgcga agcaggaccg
     2641 ccacggccac gggcccgaag cgtggaagca cgcccttgcc gtcatcgtcc acggagacgc
     2701 ggcgttcgcc gggcagggaa tggtggcgga ggtcttcaac ctcgcccagc ttcgcggctt
     2761 ccgaaccggg gggtcgatcc acctgatcat caacaaccag atcggtttca cgacgcctcc
     2821 cgcgtccggc cgctcgtcgc tctactcgac ggacgtcgcg aagatcaacc aggtcccgat
     2881 cttccacgtc aacggcgatg ctccggaagc cgcataccgc gtgctccaga tcgccctcga
     2941 ctatcggcag gagtggcaca aggacgtcgt gctcgacctg atcgggttcc gccggcacgg
     3001 acatcaggaa accgacgaac cgacctacac ccagcccctc atgtacaggc gaatccagga
     3061 gcacccgggc gtccgcgcgc tctacgcgca gaagctcgtg aaggacggcg tgctcaccga
     3121 ggtgcaggtc gcggagatgg aagagcgcca gaaggcgcag tacgaggaag cgctcgccgc
     3181 cgcccgggaa atcgcggcac gtccgacggc cgcgagcgag ccttctccga ttcccgattc
     3241 ccgatccccg agatccgatg tcgaaaccgg cgttgcccgc gagagcctcg cccgcattgg
     3301 gcgcgtcctc acgacggtgc cgccgggctt ccacatcaac ccgaagatgg tgtcgcagct
     3361 cgcccgccgc gcgaaaatgg ccgagggcag cgagccgctc gactggtcca cggcagaggc
     3421 cctcgcgttc ggctctcttc tcctcgaggg gacgcccatc cggctgaccg gacaggacac
     3481 gagccgcggg acgttctccc agcgccacat cgtcttccac gacacggcga ccggcgatac
     3541 ctggacgccg ctttgcgacc tggacccggc gcggccgaaa cttgccgttt acgacagccc
     3601 gctgtcggaa gtgagcgttc tggggttcga gtacggatac agcgtcgagg cgccggagac
     3661 gctcgacctg tgggaagcgc agtatggcga cttcgcgaac ggcgcgcagg tcgtcctcga
     3721 ccagttcgtc acgtccgccg aagacaagtg gcagcagacg tcacgcctcg gcctgcttct
     3781 gccgcacggc tatgaagggc aggggccgga gcactcgagc gcgcgcatcg agcggtatct
     3841 ccagctctgc gccgaagaca acatccaggt ctgcaacgtg acgacaccgt cccagtactt
     3901 ccacctgctg cgcaggcaga tgcgccagga ggcggccaag ccgctcgtga tcttcacccc
     3961 caagagcctc ctgcggtttc ccgcgtcgtt ctcgccgctc gaggatctga cgcgcggatc
     4021 gttcctctcg gtggtggacg acccggaggt cgcggatcgc gccgcggtcg cgcgcgtgct
     4081 tctctgcagc gggaagatct tctacgacct gcacgctgca cgcgaggaga ggaagacgaa
     4141 cgcggtcgcg atcgtccgcc tcgagcagct ctatccgttt gccgcggacg cgcttcgggc
     4201 cgcgctcggg cgctatgtct cggcgcgcga cttcgtctgg gtccaggagg agccgcagaa
     4261 catgggcgcg tggtctttcg tacgaccccg gcggtccgag ttcctcccga ccggcgccgc
     4321 gctctggtac gcgggccggg cgccctccgc gtccccggcg accgggagcg ccgccgtgca
     4381 caagagggag ctcgaacagc tcatcgcgga ggcgttcggg cggtagcgga tccgcgactt
     4441 cgtacgagca ccgttcgcgg agagcgtaga agcgaggcgc tagggtttgc gcttcctcgg
     4501 acgcatcctc tcaagcgcct ccttgaagac ctcgcgcggg atcttgcggt agtccgagaa
     4561 ctcgccggct ccggccatcc actccccgcc gtccatcgtg acgcagtcgc cggtctggta
     4621 cgggcagagg tcgctcaaga gaaacgtcac gaggttcgcg agctcgtcgg ccgttccgaa
     4681 tcgaccgagc gggatcctct cgagcgtctt cttctcggcc tccgggaacg ccatgagccg
     4741 cgaccacgcg ccttcagtcg ggatcggacc gggcgcgacg gcgttcagac ggatgcccgc
     4801 gtgcccccac tcgacggcga gcgagcgagt catcgcgagc acgcccgctt tcgcgcacgc
     4861 ggaaggcaga gcgaacccgg tgccggtcca ggcgtacgtc gtcaccaccg agacgatgga
     4921 gccgggcgcc ttgcgctcga ggaggtgacg gccgaccgca agggtgcagt agaccgagcc
     4981 gtggagcacc gtgcggacga cggcgtcgaa gccgttcggg gtcaggtcct cggacgtgac
     5041 gaggaagttt cccgccgcgt tgttgacgag cgagtcgatc ttcccgaacc gggctatcgc
     5101 gtgtgcgacg agggcttcga cctccgccgg ctcgcgaacg tccgcggggt gaaagtcggc
     5161 ttcgccgccg gcgctcgcga cggcgcggat ctctccggcc gcccgctcga gacggtcgag
     5221 cgtacggccc gcgatcgtga ccttcgcgcc gtgcgcggcg aacttcttcg ccatcgcgag
     5281 cccgaggccc gagccgccgc cggtcacgag gatcgatctt tccgcgagga tcttcgtatc
     5341 gaacattgca agccatgggg acgggtcccg aaaggacctg cacccatctc agtgctccac
     5401 tacgatagag acgggagaca cgacatggca aacgaaaacg tcagccgccg atccctcctt
     5461 gcccgggttg tcgctctgcc ggccgcgctg gccctcgcct cgaggcccgc gcgacccgaa
     5521 gaggcctgcg ccgcgacgga ggccgacttc caggggccga tgtatctcgc cggagcgccc
     5581 cggcggtcgg tcctggccgg gccgaaggaa ccgggcgagc gcctgaccat ccggggaacc
     5641 gtcttcggct ccgactgccg caccccgctg ccccggacgc tgctcgacgt gtggcaggcc
     5701 gacgcgcagg gggagtacca ctggaaggac gaggactatc ggctgcgggg ccagctcctg
     5761 acgaacgacc ggggggagta cgagatcgcg accatcaagc ccgccggtta tggcggtcgc
     5821 ccggcgcaca tccacttcac gatcgcggcg cccggacacg cgtcgctgac gacgcagctc
     5881 tatttccggg gggatccccg cctcgaccac gacgtgtgcg gggcggcgtg caactccgac
     5941 gatccgcacc ggatcgtcga gctcgcaaga gagccgcgag gcctcgtcgg aaggttcgac
     6001 gtcgtcctga agtccgcgcg cgcctagttg ggcttcttct ccgtcacccg gacctcggga
     6061 accggcgtct tgcggacgag gaagtcctcc tcgacgtagg gtccgtcgcc gaggctgatc
     6121 cgcacggcat aggtcccttc gatcgtcagc agcgccgggc ccatcacgac cggaaaccag
     6181 atcgtgcgtc cggcctcccc ttcgtagttc aactcaccgt tcgcgtcgag aatcttgttc
     6241 ccctgcggat cggagaccga gaccgaaaag ggaaactttc cggtctcgag gaccttcgcc
     6301 ttgatagcga ggccgatcga tcgcatctgg agcggcagct tcaccacctg gacgacgtct
     6361 gagtagacgc cgatcaggag cagcttcccg ttgttctcga agcggacgtc gtcgcacagc
     6421 acgagcgcct cgaagacgat cgaaacctgc ggttctatcg cgcgccccct ttcaagtact
     6481 cgtcgaacca tttcaccagc cgcgtgtcac ggtcgagctg gtgcttcgga tcctcgatcg
     6541 cgtgcccctc gttctcgtag atgacgaggg tcgtcggaac gccgagcgtc ttcagggcgt
     6601 gccagaactc gtagccctgg ggagtcggaa cctccgagtc ccgatcgccg tgcaggacga
     6661 gcgtcggcgt ctttgccttc ttgatgaacg tgatcggcga cgagcgcgcg tagacctccg
     6721 ggtcgtcgta gaccgaagcg ccgaagaacg gcagcagcca ctggtcgatt cggttctggc
     6781 cgtagtagct ctgccagctc acgatcccgg cgcccgccac cgccgccgcg tagcgttccg
     6841 tctgcgtcac cgcccacatc gtcatgtacc cgccgtaact ccagccgcag atccccagcc
     6901 gcttcggatc gatcggagca gccgcgagag cggcgtccac acctcggtcg atgtcgcgga
     6961 agtcgccgta cccgaagtcc ttcacgttgc ctcgagtgaa ggcttcgccc tggccgaagc
     7021 tcccgcgcgg gttggggagg aagacgtaat agccctggct cggcaggatc gcggtccagc
     7081 ggctgggcca gctcgccgtc gaggacccgg aaggacctcc gtggaccacg acgaccatcg
     7141 gggatttctt cgactcgtcg cgatcgagcg gcgaaaggag ccagccctgc acgcgctgtc
     7201 cgtcgctctg ccatgtcagg ctccgggctt cgccccagaa cggccggact ccggcgttgc
     7261 ctcgcgtgag aggcgcccag gtgccgatcg gtcccgcgac gatctcgggc ggctgcgaga
     7321 aagaggaacg gatcgcggcg gatgccgatc cctccctcgc gaccgagaac ctcttgacga
     7381 ccgccggtcc gcgccagagc gtccggacgt ttcccgaggc gtcgagcgcc gcgaccccct
     7441 cgtcgccgtc cacgtactcg gtgaacagga tctcgccggg agacctccag aagattgccg
     7501 acgccgaggc ggcaattccc tcggtccggt ttcgcgccgg gccgcccgcc acggggacgg
     7561 tccagatatc gccgccggtc aagccctcgt cgctcatgat cccgtggatg acggcgatct
     7621 cctttccatc gggcgaccac ctcggacacg cgatctggag cggcggcttc cagagcgact
     7681 tcgtctcgcc gctcgccgcg tcgaccgtgt agagctgcgc gatccagtag ttgttggtgc
     7741 cggaaccttc ggcggcctcg gcggcgaacc gtttgccgtc gggggaccag tcgtagtcgt
     7801 agacgtacag attggcgggg gagatcgacc gcacctttcc cgtgtccgcg tccaggaccg
     7861 cgatccgctg ctcttcgatc gtctcggcga cgacgccggc atccggcttg taggccgtga
     7921 gcgcgccggt ctcctgcgcc gagccttcga cgaagagaaa cgcgatggag tggccgtccg
     7981 gcgaccagcg aaggtccgac agctggccct tcacgctggt cgtccggcgg ggcggccctc
     8041 cctcggccgg cgcgacccag acctgcatct gtttttcttt cgcggcgtcg gagagaaacg
     8101 cgatgcgagt cgagtccggc gaccaggccg gcccgcgttc gtcgtgcgcg cggcctcccg
     8161 cgccggccgt gactcgccgg gacttgcgcg aaggcgctgt caggtcggaa acctcgatgg
     8221 ccgcgagctt ccaggcgccc ctggcgtcct tgatcttccg aacccaggcg acgcgtttgc
     8281 catctggtga gatcgtcgcg gcgtcgtagt cctggacgct ctggagcgtc gcgagaacgg
     8341 actcgatgga cgggcggccg gccggagtcg cgaaggcgga gatcgtccac gccgcgagag
     8401 cgagggcggg gaggatcttc ttcacgagcg aaggatatga cggaggcgtc gcgtgcgcgg
     8461 atctacgtga gatccaccca gacgctcttg acctgcgtgt actcctccag cgcgtagtga
     8521 ccgcgctcgc ggccgaagcc ggactccttg tagccgccga agggtagccc ggggtcgtag
     8581 tagttgtacg tgttcaccca cacggtgccg gccttcaacg cctttgcggt gcggaaggcc
     8641 ttcttcacgt ccttcgtcca gacggcggcc gcgaggccgt acaccgtcga gttaccgatc
     8701 gcgaccgcgt ccgcctcgtc ctggaacgtc agcgtcgcga gcaccggccc gaagatctct
     8761 tcgcgggcga tcttcatctc cgggcggacg ccgtcgaaga cggtcgcctc gaagaacgcg
     8821 cctttcccgt caacggcggc acggttcccg cccgcgacga gcttcgcccc ctcgcgcttt
     8881 ccgctctcca cgtaccgctc gacgttctcg cgctgcttct ccgagacgag cgaccccaac
     8941 cgggtcttcg ggtgaagggg gtccgccggg atcatctttt tcgcgcggtc gacaaccttt
     9001 tcgacgagcg cgtcgtgcgc gccttcctcg acgaggagac gcgaccccgc cgagcacagc
     9061 tctcccttgc cgtagaagat cgcgttgatc gcgcccttcg cagcggcatc gaggtccgcg
     9121 tcggcgaaga cgacgttcgg agacttgccc ccgagctcca tcgagacgcg cttgaccgtt
     9181 ccggcggccg ctcgcaggat cgccttgccc gtctgcgttt cgccggtgaa ggagatcttg
     9241 tcgatgccgg ggtgctccgc catcgcggcg ccgacctgcg ccccgggacc cggaacgacg
     9301 ttgaacgctc cgggaggcag tcccgcctcg cggaggaggt cggcgaagag gagcgcagtc
     9361 agcggggtga gcgaagccgg cttcaagacc acgctgttgc cgagcgcgag cgccggcgcg
     9421 atcttccaca tcgcctggag gagcgggaag ttccacggcg tgatcgcacc gacgacgccg
     9481 accggctcgc gcatcgtcat gaggaaggcg tccgaccgcg cggggaacgt gtcgccgtag
     9541 agcttgtcgg cgtactcgcc gaagtaggag agcgcctccg cggcgaccgg catgtcgatc
     9601 ttcgcggact cgaagatcgg ctttccgttg tcgagcgtct cgacccgccc gaattcctcg
     9661 gcgcgggaga ggagcaggtc cgcggccttg atgagaatcc gcccgcgctc gcgcggcttc
     9721 atcgcggccc acgggccgga ctcgaaggcg tcgcgcgcgg cgtcgaccgc gcggtcgagg
     9781 tcttccttcc cggcctccgc gacctcggcg atccgctctc cggtcgcggg attcgtcgtc
     9841 gagaagcgct ttcccgacgc ggacggaacg aactcgcctc cgatgaagag gtcggtcttg
     9901 agcgacagcc ccggaaccgt ctccgccgcc acttaccgcc ctttgaactt cgggacccgc
     9961 ttctcctggt tcgcggcgat gccttccttc gcatcgtcgc tctggaagag gagctgctgg
    10021 agctcgcgct cgacggcgag cgccgactcg agcggcaggt cccagccgga ctgcaccgac
    10081 cgcttgatcc ggccgacggc cttcgaagcc ttgttcggcg gaacgaactg gcgcgcgtag
    10141 tccatcacct gctcccacca ctccgccggg gtcgcgtcga tgacctggtt gacgaggccg
    10201 aggtccgccg cttcctcgaa ggaaaacgtg ttgccggtga tcatgagctc gatcgcgcgg
    10261 gacttgccga tcaactttcc gagccgggcc gtgccgccgg tccccggcag aacgccgagc
    10321 gtcacctcgg gcagcccgat cttgccggcg tccttgcgcg cgatccggat gtcggccgcc
    10381 atcgcgatct cgaggccgcc gccgaccgtg tgcccgttca acgccgcgat cacgagcttt
    10441 ggcgtgtgct cgagccggag cagcgtctcg ttggcgtgga ggcagaagaa gtacttgaag
    10501 ccgggcgtga ccgcgttcag catcccgatg ttcgcgcccg cggagaagaa gcggtcgccc
    10561 ttcccgcgga ggacgatgac gtggacgtcg tcgtcgaaac gcgccttcag gatcgcgtcg
    10621 tcgagctgcc gcatcatcgc gtaggtgtag gtgttggccg gcggatcatc catttcgacg
    10681 atcgcgatgc ccgaatcggc gcgatagtgg acgatttctg acccgacctc cgggctcgtc
    10741 cagtcgcgcg tttgaaccgg tgtactcatt cgaaacctcc cgtcagcgga ccgccgatcc
    10801 tatcagcccc gcgtcgggtc gacccactcc gacgatccgt ccgggcggat ttcccttttc
    10861 cagatcggga cggtcttctt gacccgctcg atcgcctcgc ggcaggcggc gaatgcctct
    10921 tcgcggtgcg gggccgtggc gacgattgcg accgacggct cgccgaccgc gagccgcccc
    10981 acccggtgcc ggatccggag aaagactccc ggccactccg tcgtgagcga atccgcgatc
    11041 cgcgccatct ccgattccgc catcgccccg tacgcctggt actcgacggc gacgctctgg
    11101 cgtccgtggc tctcgttgcg cacgaccccg acgaagacgc agaccgcgcc gtcggagggg
    11161 cgcgcctcac ccaggaaagc cgcgagatcg atcggctcgc tcgacaggaa catcgaaccc
    11221 ctaacccccc gcgacggggg gaaggaacgc cacctcgtcg ccgtcgtgga gctccgcctc
    11281 ggccgaggcg tactcgcggt tgatggctgc cgccggcatg gagagaaagc gcgaaaagcc
    11341 gggcacgtcc cgcgcgagct cctgccagag ctcgccgacg cggcatccgg aagggagttc
    11401 ccgagcctct tcatcccgcc cggcagcgtc gcgaaacgag gcgaaataca ggacgcggac
    11461 ctccatcggg aggagtctac tttcgccgga gtggcttgac atctgaccga ccggtcagat
    11521 agtctttcgg tgatggccag cagccgccct actccccgcg agaccgagcg acgcgtcgct
    11581 ctcctgcgag ccgccttccg cgaagtcggg gagaaaggct tctccgaggt gacgctcgac
    11641 gacatcgccc accgggcggg ggtttccaag ggcgtgacgc t
//
LOCUS       DASWAV010000661         1951 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U2665
            k141_479291, whole genome shotgun sequence.
ACCESSION   DASWAV010000661 DASWAV010000000
VERSION     DASWAV010000661.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960316
            Sequence Read Archive: SRR10260007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 1951)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1951)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:47:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,929
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1951
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_479291"
                     /isolate="SMAG_U2665"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.36444 N 132.31889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..198
                     /locus_tag="VF958_13900"
     CDS             <1..198
                     /locus_tag="VF958_13900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9150249.1"
                     /translation="IRLTDPPASEPNPDREIPYLLCKECGTPCYVFETEKGSVREALC
                     IACGNEAVARFALGDWEDDEG"
     gene            185..1615
                     /gene="purB"
                     /locus_tag="VF958_13905"
     CDS             185..1615
                     /gene="purB"
                     /locus_tag="VF958_13905"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020073904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004018 - N6-(1,2-dicarboxyethyl)AMP
                     AMP-lyase (fumarate-forming) activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HEX9150250.1"
                     /translation="MTKADRDASPLWSRYASAEMADLFSAKRKFETWRLLWLWLAEEE
                     QRLGLPISDEAIGQMRAHLTDIDFDAAAREEERIRHDVMAHVKVFGKAAPAARPIVHW
                     GATSAFVTDNADLIVVRDALGLVERRLAAVLARMRALALEHRATPALAYTHYQPAQPT
                     TVGKRIALWASDLVGDLEDIRRRKEDLRFLGAKGATGTQASFLLLFDGDAARVEELDR
                     ALARRAGFPKRQIVSGQTYSRKQDDRVVHALSGLAQSAHKIGTDLRLLQHDGEIEEPF
                     ESAQVGSSAMPYKRNPMRAERICSLARHVIALSFDTAMTAATQWLERTLDDSANRRIA
                     IPEAFLTADAILVLLENVFSGLVVHADVARRRLEAEIPFLATENILMEAVRRGGDRQE
                     LHEKLRVFVRAAAERPAGTGRATDLLDRIAADADFGLTRAELDQAARPERLTGRSAEQ
                     VETFVREELDPALAGVEAVASVPLKV"
     gene            1612..>1951
                     /locus_tag="VF958_13910"
     CDS             1612..>1951
                     /locus_tag="VF958_13910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020297120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septal ring lytic transglycosylase RlpA family
                     protein"
                     /protein_id="HEX9150251.1"
                     /translation="MTPRDAVRVALLVAVLGAVAACAAHPPARSVSHAPPAPGWTEEG
                     VASWYGGRDGFEGKPTASGEIYDSSLLTAAHRELPLGTVVDVTNLDNERTVRVRINDR
                     GPFVFGRVIDL"
BASE COUNT          309 a          690 c          666 g          286 t
ORIGIN      
        1 atccgcttga ccgacccacc cgcctccgag cccaacccgg atcgcgagat tccctacctg
       61 ctctgcaagg agtgcggcac gccctgctac gtcttcgaga ccgaaaaagg cagcgtgcgg
      121 gaggcgctct gcatcgcctg cggcaacgag gcggttgcaa gattcgccct cggggactgg
      181 gaagatgacg aaggctgacc gcgacgccag cccgctctgg tcgcgctacg cgtcggcgga
      241 gatggccgac ctcttctcgg cgaaacgcaa gttcgagacc tggcggctcc tgtggctctg
      301 gctcgccgaa gaggagcagc ggctcggtct tccgatctcc gacgaagcga tcgggcagat
      361 gcgcgctcac ttgaccgaca tcgacttcga cgccgcggcc cgcgaagagg agcggatccg
      421 ccacgacgtg atggcgcacg tcaaggtctt cggaaaggcc gcgccggccg cgcgcccgat
      481 cgtgcactgg ggcgcgacgt cggccttcgt cacggacaac gctgatctca tcgtcgtccg
      541 cgacgctctc gggctcgtgg agaggcggct cgcggcggtt ctcgcgcgga tgcgcgcgct
      601 ggcactcgag caccgggcga cgcccgcact ggcgtacacg cactaccagc cggcgcagcc
      661 gacgaccgtc ggaaaacgga tcgcgctgtg ggcgtcggac ctcgtcggcg acctcgagga
      721 catccggagg cggaaagagg acctgcggtt tctcggggcg aaaggggcga ccggcacgca
      781 ggcgtcgttt ctgctcctct tcgacggcga cgcggcgaga gtcgaggagc tcgaccgcgc
      841 cctcgcccgc cgcgccggct ttccgaagcg gcagatcgtc tccggacaga cctactcgcg
      901 caagcaggac gaccgggtcg ttcatgcgct ttcggggctc gctcaatcgg cccacaaaat
      961 cggaacggac ctgcgcctgc tccagcacga cggcgagatc gaggagccct tcgagtccgc
     1021 gcaggtgggc tcctccgcca tgccttacaa gcgcaatccg atgcgcgccg agcggatctg
     1081 ctcgctcgcg cggcacgtca tcgctctctc gttcgatacc gcgatgaccg cagcgaccca
     1141 gtggctcgaa cggaccctcg acgactcggc gaaccggcgc atcgcgatcc ccgaggcgtt
     1201 tctgaccgcg gacgcgatcc tcgtcctcct cgagaacgtc ttctcgggac tcgtcgtgca
     1261 cgcggacgtc gcgcggcgac gactcgaagc ggagatccca ttccttgcga ccgagaacat
     1321 cctcatggag gcggtccgcc gaggcggcga ccgccaggag cttcacgaga agctgcgcgt
     1381 attcgtgcgc gccgcggccg aacggcccgc gggcaccgga cgggcgaccg atctgctcga
     1441 ccggatcgcc gcggatgccg acttcggtct gacacgcgcg gagctcgacc aggcggcgcg
     1501 ccccgagcgc ctgaccggcc gctccgccga gcaggtcgaa acgttcgtgc gggaagagct
     1561 ggacccggct ctcgcgggcg tcgaagcggt cgcgagcgtt cccctgaagg tgtgacgccg
     1621 cgcgacgcgg ttcgggtcgc cctcctggtc gccgtgctcg gagcggtcgc ggcgtgcgcc
     1681 gcgcatcctc cggcacgaag cgtttcgcac gccccccccg cccccggctg gacagaagaa
     1741 ggggtcgcgt cgtggtacgg cggccgcgac ggcttcgagg gaaagccgac ggcgtcgggc
     1801 gagatctacg actcgtcgct cctgaccgcg gcccaccgcg agctccctct cggaacggtg
     1861 gtcgacgtca cgaacctcga caacgagcgg accgtccggg tgcggatcaa cgaccgtggg
     1921 ccgttcgtct tcgggcgcgt gatcgatctc t
//
LOCUS       DASWAV010000662         5831 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U2665
            k141_757818, whole genome shotgun sequence.
ACCESSION   DASWAV010000662 DASWAV010000000
VERSION     DASWAV010000662.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960316
            Sequence Read Archive: SRR10260007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 5831)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5831)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:47:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,929
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5831
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_757818"
                     /isolate="SMAG_U2665"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.36444 N 132.31889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(69..620)
                     /gene="rpsE"
                     /locus_tag="VF958_13915"
     CDS             complement(69..620)
                     /gene="rpsE"
                     /locus_tag="VF958_13915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013259712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HEX9150252.1"
                     /translation="MSRAPREREKERDAQGRELIDRVVHINRVTKVVKGGKNFSFSAL
                     VVVGDGQGRVGYGSGKAKEVPTAIKKGIEMAKKNMQQISLKGSTIPHAVLGRYGAGRV
                     LLKPAAPGTGVIAGGPVRAVLESVGIQDILTKSLRTTNPHNVIKATFEGLTRLKNEDE
                     IRKRRGVAGVTGAREAETVEAEA"
     gene            complement(667..1035)
                     /gene="rplR"
                     /locus_tag="VF958_13920"
     CDS             complement(667..1035)
                     /gene="rplR"
                     /locus_tag="VF958_13920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013074240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HEX9150253.1"
                     /translation="MNRSEVRTEARRRIRSRIRRRVKGSGERPRLAVFKSGRHIYAQV
                     IDDATGATIAHASSLDAALKGEKKPAANRETATRVGTLVAERAKEKGVARVVFDRGGY
                     IYHGKIKALAEAARQGGLEF"
     gene            complement(1038..1580)
                     /gene="rplF"
                     /locus_tag="VF958_13925"
     CDS             complement(1038..1580)
                     /gene="rplF"
                     /locus_tag="VF958_13925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011522045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HEX9150254.1"
                     /translation="MSRIGRMPIGIPQGVEIRADGDYVRVKGPKGELSSRIPPGIAVE
                     VDKGEVRFTRSNDEPQQRAWHGLVRSLVANSVAGVTKGFTRDLEIVGVGYKAEVKGKT
                     IVFTLGYSHPINFPIPDGINVALDAKAGKLSISGADRQKVGQTAAEIRKLRIPDPYKA
                     KGIKYADEVIRRKVGKAGGK"
     gene            complement(1607..2005)
                     /gene="rpsH"
                     /locus_tag="VF958_13930"
     CDS             complement(1607..2005)
                     /gene="rpsH"
                     /locus_tag="VF958_13930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007285300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HEX9150255.1"
                     /translation="MKTSDPLSDLLNRLRNGMRAGHDRVDVPASRIKEDVLRVLADEG
                     YVSSWRRVEEKGRPVLRIGLKYDHEGSPIVAGLKRVSRPGRRVYTPAKEIPDVLGGLG
                     ISIISTSKGIVTGRKARESRLGGEILCNVW"
     gene            complement(2010..2195)
                     /locus_tag="VF958_13935"
     CDS             complement(2010..2195)
                     /locus_tag="VF958_13935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015337847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type Z 30S ribosomal protein S14"
                     /protein_id="HEX9150256.1"
                     /translation="MARRAMRIKAARKPKFMTRTVRRCRVCGRARSVLRKFGLCRICF
                     RANALSGYIPGIVKASW"
     gene            complement(2213..2767)
                     /gene="rplE"
                     /locus_tag="VF958_13940"
     CDS             complement(2213..2767)
                     /gene="rplE"
                     /locus_tag="VF958_13940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020709050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HEX9150257.1"
                     /translation="MEPRLKEQYDRQIIPDLKKELGEDNVMALPRLDRIVLNMGLGEA
                     VANPKILEGAVEELSAIAGQRAVVTKAKKSIATYKLRTGMPIGARVTLRGRRMYDFLD
                     RLINIALPRVRDFRGVSPKAFDGRGNYTLGVRDHFIFPEIDYSRTDKSKGLNVTIVTT
                     AGRDDRALALLKRLGMPFASRTQT"
     gene            complement(2800..3108)
                     /gene="rplX"
                     /locus_tag="VF958_13945"
     CDS             complement(2800..3108)
                     /gene="rplX"
                     /locus_tag="VF958_13945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013581282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HEX9150258.1"
                     /translation="MKKNDVVVVIAGRDRGRQGKILRVLPREDRVIVERVNMIKRHVR
                     PNPAKNIAGGISEKEASFHISNVMLVDPDRNTPTRIGRRRDADGTPERFAKKSGAALA
                     "
     gene            complement(3120..3497)
                     /gene="rplN"
                     /locus_tag="VF958_13950"
     CDS             complement(3120..3497)
                     /gene="rplN"
                     /locus_tag="VF958_13950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013709164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HEX9150259.1"
                     /translation="MIQMGTILEVADNSGARKIACIRMRGGSAGRYAGLGDVITASVK
                     EAAPDSPFVEKHGKRVVKAVIVRTRRNHRRRDGSYIRFDDNAAVLISPEGEPVGTRVF
                     GPVARELREKKFMKIISLAPEVL"
     gene            complement(3507..3785)
                     /gene="rpsQ"
                     /locus_tag="VF958_13955"
     CDS             complement(3507..3785)
                     /gene="rpsQ"
                     /locus_tag="VF958_13955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008474378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HEX9150260.1"
                     /translation="MEATANQAPSRGRATKVGRVVSDAMKKSIVVEVERHVEHGRYKR
                     TVKRTSRFMAHDEANTAKAGDRVRIEETRPMSKRKRWILKEVLVRAEA"
     gene            complement(3785..4003)
                     /gene="rpmC"
                     /locus_tag="VF958_13960"
     CDS             complement(3785..4003)
                     /gene="rpmC"
                     /locus_tag="VF958_13960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013119215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HEX9150261.1"
                     /translation="MKKKERETLSQQGEADLIEREKQLRESSWKLRLQKATGQLENPM
                     RLKVLRRDIARVKTYRRALERRAEPEKQ"
     gene            complement(4000..4425)
                     /gene="rplP"
                     /locus_tag="VF958_13965"
     CDS             complement(4000..4425)
                     /gene="rplP"
                     /locus_tag="VF958_13965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014263814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HEX9150262.1"
                     /translation="MLMPSKVKYRKQQRGRMRGKAYRGGSLAFGDTGLQVLEPGWVTA
                     RQIEAARIAMTRAVKRGGKIWIRIFPDKPYTKKPAETRMGKGKGPPEGWVAVVKPGRI
                     LYEMEGVDLATAKEALGLAADKIGLKTRFITRESEMEIR"
     gene            complement(4467..5117)
                     /gene="rpsC"
                     /locus_tag="VF958_13970"
     CDS             complement(4467..5117)
                     /gene="rpsC"
                     /locus_tag="VF958_13970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014263813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HEX9150263.1"
                     /translation="MGQKVHPYGFRLGFNRTWHSRWFASKDYQTYLHEDLKLRKELKH
                     RLSHAGVSEIDIERFANKLKVNIQTSRPGIIIGKRGAEVDKLRDELQKRMKGEVHINI
                     IEIQRPETDAQLVAESIALQLERRVAFRRAMKKGIESAFRFGAQGIKIRCAGRLNGAE
                     IARAEWYQEGRLPLHTLKADIDYGFAEANTTYGKIGVKVWIYRGEQHKAKNRRKTA"
     gene            complement(5126..5473)
                     /gene="rplV"
                     /locus_tag="VF958_13975"
     CDS             complement(5126..5473)
                     /gene="rplV"
                     /locus_tag="VF958_13975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011698417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HEX9150264.1"
                     /translation="MKAKATLRYRKGSAQKARLVADLVRGKPVNDALAILRHTPKVAA
                     RDIEKTLRSAVANAEQKEAHLDVDELVVRKIFVDKGPQDKRVRPAPMGRAFRVLHRKS
                     HLTIEVSNEGQEK"
     gene            complement(5581..5831)
                     /gene="rpsS"
                     /locus_tag="VF958_13980"
                     /pseudo
     CDS             complement(5581..5831)
                     /gene="rpsS"
                     /locus_tag="VF958_13980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018049714.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="30S ribosomal protein S19"
BASE COUNT          778 a         2038 c         1805 g         1210 t
ORIGIN      
        1 gctcgccgcc ggcttgcggg cgcgccgctt tcctgcggcg ggtgccgtgg agctcgtcgg
       61 agattcgctc acgcctcggc ctcgaccgtc tccgcttccc gcgcgccggt cacgccggcc
      121 acgccgcgac gcttccggat ctcgtcctcg ttcttcaggc gcgtcaggcc ctcgaacgtc
      181 gccttgatga cgttgtgcgg gttcgtcgtg cggagggact tcgtcaggat gtcctgaatt
      241 ccaaccgact cgaggaccgc ccgcaccgga ccgccggcga tgacgccggt tccgggcgcg
      301 gcgggcttca gcagcacgcg ccccgcgccg tagcgtccga gaacggcgtg gggaatcgtc
      361 gagcccttca acgagatctg ctgcatgttc ttcttggcca tctcgattcc cttcttgatg
      421 gccgtcggaa cctccttcgc tttgccggag ccgtaaccga cgcgaccctg tccgtcgccc
      481 acgacgacga gcgccgagaa cgagaagttc tttccgccct tcacgacttt cgtgacgcgg
      541 ttgatgtgga ccacccggtc gatcagctcg cgaccctggg cgtcgcgctc cttctcgcgc
      601 tcgcgaggcg cccgggacat gcggttgctg ctcattggat cggtactctc cttcttcccg
      661 cgtcgctcaa aactcgaggc cgccctgtcg ggccgcctcg gcgagagcct tgatttttcc
      721 gtggtagatg tagcccccgc ggtcgaaaac gacccgggcg acgccctttt ccttcgcgcg
      781 ttcggcgacg agcgtgccga cgcgcgtcgc ggtctcccgg ttggcggccg gtttcttctc
      841 gcccttcagc gccgcgtcga gggaagacgc gtgcgcgatc gtggcgcccg tggcgtcgtc
      901 gatcacctga gcgtagatat gccgcccgct cttgaatacc gcgaggcgcg gccgctcgcc
      961 cgaccccttg acgcgccgcc ggatgcgcga gcggattcgc cggcgggctt ctgttctgac
     1021 ttcggaacgg ttcatcgcta ctttccgccc gcctttccga ccttgcgccg gatcacctcg
     1081 tcggcgtact tgatgccctt cgccttgtac gggtccggga tacgcagctt gcgaatctcg
     1141 gcggccgtct gtccgacttt ctggcggtcg gcgccggaga tcgagagctt gcccgccttc
     1201 gcgtcgagcg cgacgttgat cccgtcggga atcgggaagt tgatggggtg cgagtagccg
     1261 agcgtgaaga cgatcgtctt ccccttgacc tcggccttgt aaccgacgcc gacgatctcg
     1321 agatcgcggg taaacccttt cgtgaccccc gcgaccgagt tggcgacgag gctgcggacg
     1381 agtccgtgcc aggcacgctg ttgcggctcg tcgttcgagc gggtgaaacg gacctcgccc
     1441 ttgtcgactt cgacggcgat gcccgggggg atccggctcg agagctcgcc cttcggtccc
     1501 ttcacccgga cgtagtcccc gtccgcgcgg atttcgacgc cctgcgggat cccgattggc
     1561 attcgtccga tacgtgacac ggttactcct cgcccttctg ccagctctac cagacgttgc
     1621 agaggatctc gccgcccagg cgcgactcgc gcgccttccg gccggtgacg atccccttcg
     1681 acgtcgaaat gatcgaaatt ccgaggcccc cgagcacgtc gggaatctcc ttcgccggcg
     1741 tgtagacgcg tcgccccggc cgcgagactc tcttcaaccc cgcgacgatc ggagaaccct
     1801 cgtggtcgta cttcagcccg attcggagca ccggccggcc cttctcttcc actcggcgcc
     1861 aggaggaaac gtatccctcg tcggcgagca cgcgcagcac gtcctccttg atacgcgagg
     1921 ccgggacgtc gacgcggtcg tgaccggcgc gcatgccgtt gcggagccgg ttcaggagat
     1981 cggaaagcgg atcgctcgtc ttcatcgcct caccacgacg ccttcacgat gcccgggatg
     2041 tacccggaca gcgcgttcgc gcggaaacag atgcggcaga ggccgaactt gcgcaggacg
     2101 ctgcgcgccc ggccgcagac ccggcagcgg cgcacggtcc gtgtcatgaa cttcggcttc
     2161 ctggccgcct tgatcctcat tgctcttctg gccatttcct ggttcctctg tcttaagtct
     2221 gcgtccggga tgcaaacggc atcccgagcc gcttcaagag cgcgagcgcc cggtcgtcgc
     2281 gccccgcggt cgtcacgatc gtgacgttca atcctttcga cttgtccgtc ctggagtagt
     2341 cgatctccgg gaagatgaag tggtcgcgaa ctccgagcgt gtagttcccg cggccgtcga
     2401 acgctttggg cgacacgcct cgaaagtcgc gcacgcgcgg gagagcgatg ttgatcaggc
     2461 gatcgagaaa gtcgtacatg cgccggccgc gcagcgtcac gcgggcgccg atcggcatgc
     2521 cggtacggag cttgtacgtc gcgatcgact tcttcgcctt cgtgacgacg gcgcgctgtc
     2581 cggcgatcgc cgagagctcc tcgacggccc cctcgaggat cttcgggttc gcgaccgcct
     2641 cgccgagccc catgttgagg acgatccggt cgagccgcgg caacgccatg acgttgtcct
     2701 cgccgagctc cttcttgagg tcgggaatga tctgccggtc gtattgctct ttcagtcttg
     2761 gttccatgtc ttctctctcg tctggtctta ctctcccggc taggcgagcg ccgcgccgct
     2821 cttcttcgcg aagcgctccg gggttccgtc ggcgtcgcgg cggcggccga tgcgcgtcgg
     2881 agtgttgcgg tccgggtcca cgagcatcac gttcgagatg tgaaacgacg cctccttctc
     2941 cgagatcccg cccgcgatgt tcttcgcggg gttcggccgg acgtggcgct tgatcatgtt
     3001 cacgcgctcg acgatgacgc ggtcctccct gggaaggact ctcaagatct ttccctggcg
     3061 gccgcggtcc cgccccgcga tgacgaccac gacgtcgttc ttcttcaagc gaggcttctt
     3121 cacaggacct ccggggcgag cgagatgatc ttcatgaact tcttctcgcg cagctcgcgg
     3181 gccaccggcc cgaatacgcg cgtgccgacg ggctcgcctt ccggggagat caggacggcc
     3241 gcgttgtcat cgaaccggat gtaagagccg tcgcggcggc ggtggttgcg ccgggtccgg
     3301 acgatgacgg ccttcacgac ccgcttgccg tgcttctcga cgaaaggcga gtcgggggcc
     3361 gcctccttga ccgacgccgt gatgacgtcg cccaacccgg cgtagcggcc ggcggatccg
     3421 cctctcatcc ggatgcacgc gatcttgcgg gcgccggagt tgtccgcgac ctccaaaatg
     3481 gttcccatct gaatcatcgc gtgctcctac gcctctgccc ggacgagaac ttctttcaag
     3541 atccagcgct ttctcttcga catcggccgc gtctcctcga tccggacgcg gtcgccggcc
     3601 ttggccgtgt tcgcttcgtc gtgcgccatg aagcggctcg tgcgcttgac cgtccgcttg
     3661 tagcggccat gctcgacgtg gcgctcgacc tcgacgacga tcgatttctt catcgcatcg
     3721 gagacgaccc gtccgacctt cgtcgcacgc ccgcgcgagg gcgcctggtt cgctgtggct
     3781 tccattactg tttctccggc tccgcccgac gctcgagcgc ccgacggtag gtctttaccc
     3841 gggcgatgtc gcgccgcagc accttcagcc gcatcgggtt ctcgagctgg ccggtcgcct
     3901 tctggagccg cagcttccac gaggactccc ggagctgttt ttctctctcg atgagatccg
     3961 cttccccctg ctgggaaagc gtctctctct ccttcttctt catcgaatct ccatctccga
     4021 ctcacgggtg atgaaacgcg tcttcaatcc gatcttgtcg gccgcgagac cgagcgcctc
     4081 cttcgccgtg gcgaggtcga cgccctccat ctcgtagagg atgcgaccgg gcttgacgac
     4141 cgcgacccac ccttccggcg gacccttgcc cttgcccatg cgcgtctcgg ccggcttctt
     4201 cgtgtacggc ttgtcgggaa agatgcggat ccagattttt ccgccgcgct tgacggctcg
     4261 agtcatcgcg atccgggcgg cctcgatctg gcgggcggtc acccagccgg gctcgaggac
     4321 ctggagaccc gtgtccccaa acgcgaggct gccgccacga tacgccttgc cgcgcatccg
     4381 accccgctgc tgtttgcggt acttgacctt ggatggcatc aacatggact agctcgtttc
     4441 ccgttttccg tttcccgttt tccggctcac gcggtcttcc ggcggttctt ggccttgtgc
     4501 tgttccccgc ggtagatcca caccttcacg ccgatcttcc cgtacgtcgt gttcgcttcg
     4561 gcaaacccgt agtcgatgtc ggctttcagg gtgtgcagcg gcagccgccc ctcctggtac
     4621 cactccgcgc gggcgatttc cgcgccgttc agccgcccgg cgcaacggat cttgatcccc
     4681 tgggctccga accggaacgc cgactcgatc cctttcttca tcgcgcgccg gaacgcgacg
     4741 cggcgctcga gctgcagcgc gatcgactcg gcgacgagct gcgcgtccgt ttccggccgc
     4801 tggatctcga tgatgttgat gtggacctcg cccttcatcc gcttctgcag ctcgtcgcgg
     4861 agcttgtcca cctccgcgcc tcgcttcccg atgatgatcc cgggccgcga cgtctggatg
     4921 ttgaccttca gcttgttcgc gaagcgctcg atgtcgatct ccgagacccc cgcgtgcgaa
     4981 agccggtgct tcagctcttt gcgaagcttc aggtcctcgt gcaggtaggt ctggtagtcc
     5041 ttcgacgcga accagcgcga gtgccaggtc cggttgaagc cgagccggaa tccgtacggg
     5101 tggactttct gtcccattga atcctctact tctcctggcc ttcgttcgac acttcgatcg
     5161 tcaggtgact cttgcggtga aggacccgga acgcccgccc catcggagcc gggcgcacgc
     5221 gcttgtcctg gggtcccttg tcgacgaaga tcttccggac cacgagctcg tcgacgtcga
     5281 ggtgagcctc tttctgctcc gcgtttgcga ccgccgagcg cagcgtcttc tcgatgtcgc
     5341 gggcggcgac cttcggcgtg tgccggagga tcgcgagcgc gtcgttgacc ggcttgccgc
     5401 ggacgagatc cgcgacgagg cgggccttct gcgccgagcc cttgcggtag cggagggttg
     5461 ccttcgcctt catgccgggg ccccctttgc ggccggcgcc gccggtggcg ccgctcccgg
     5521 cgcgccgggc gctcccggtg ctccgggcgc tccgccgagc ttcgcaccga cctcggcctt
     5581 cttcccgccg tgccccttga agatgcgcgt cggcgagaac tcgccgagct tgtgcccgac
     5641 catgttctcc gcgatgtaga ccgggatgaa cttgcgcccg ttgtgcacgg cgatggtgtg
     5701 cccgaccatg tccgggacga tggttgaccg ccgcgaccag gtcttgatca ctttccgctc
     5761 gcgggtggag ttcaactccg ccaccttctt gacgaggtgg tcgtcgatga acgcgccctt
     5821 cttgatcgat c
//
LOCUS       DASWAV010000663         3840 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U2665
            k141_239000, whole genome shotgun sequence.
ACCESSION   DASWAV010000663 DASWAV010000000
VERSION     DASWAV010000663.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960316
            Sequence Read Archive: SRR10260007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 3840)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3840)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:47:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,929
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3840
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_239000"
                     /isolate="SMAG_U2665"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.36444 N 132.31889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            56..1573
                     /locus_tag="VF958_13985"
     CDS             56..1573
                     /locus_tag="VF958_13985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9150265.1"
                     /translation="MDEASRARIRVFLNDPAARGERPVPSDPPRETERSPWAFRVQGR
                     EPQPREYEPDTPEFLHWQITSALARGRALWSRLLPKAGRWIPGDVLEAIPVDGKDLNA
                     FYDRRALHFFRDVDEKTGAVVQSGDSPDVVTHEQGHAVLDSIRPDLWDAPHFEVAAFH
                     EAFGDVAAILVALEEKELVGDVLEETGGAIDHSNLVSRLAEELGAAVAARYGPGAALP
                     DALRDAVNAFRYRAPRSLPEDAPAGDLSREPHSFSRVVTGVCWDVLVRLYASKSRVRA
                     AAATEVRVDALGLAARELGRLIAAAAANAPPGAAFFRRFAGELASAAAGAAGERTEVT
                     EALVSRSLLASAPPEPRGRGERVAAMALGAEPLPTRVLDSVRERLGAPIDEEILVRSA
                     PDGTLHGRRARDLFLSGADYGPADGAAVEISDSFAVAVEESGFVTASRVERAGPEDEE
                     DAREFVRALARTGRIAEDASLDSSALYREGKSHVVRREADGVRRLRRAWVAGGRR"
     gene            1570..2178
                     /locus_tag="VF958_13990"
     CDS             1570..2178
                     /locus_tag="VF958_13990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9150266.1"
                     /translation="MRRRSRGLGLLAAALLASACASSRETSSHPADAARISPAELLAS
                     LADVAQGRNADLAETGLVLKKVELKVVVGHDRRAGAHASFLVLDVESSRRSEVSFTQT
                     FTFDLPPPERRRSAAPPVAVPGVAEFVDAAIATARELAAVAAREDLPQRLSEVELTAK
                     IVRAQRAEGGVAFHGLGSASLGGGVAGSSEESNTVKLVFGRR"
     gene            complement(2242..2544)
                     /locus_tag="VF958_13995"
     CDS             complement(2242..2544)
                     /locus_tag="VF958_13995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014802421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HEX9150267.1"
                     /translation="MAEKSGKALSKSQIADKLAQAGGQTKRTATQFLDDLTSLAYKEA
                     KNSFTIPGIGKLVLVNRKARMGRNPATGEEIKIPAKRVVKFRVSKKAKDAILGAKK"
     gene            2753..>3840
                     /locus_tag="VF958_14000"
     CDS             2753..>3840
                     /locus_tag="VF958_14000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9150268.1"
                     /translation="MTPRRRRRLGEIAAVVLLVTPLACRLDGDRKVAGRILDRHRRAT
                     GAKPLPASHVIRMRLSSETPGGGTGVAEVAWEPNRFRERIASAGATTERGIQGGKAYF
                     VDEDGVTRVGSEPVLRELLARSYFWRRAWLFADQGGARLSLGPADPSSVSVRLQPFGS
                     NPLLLSFSRRDGRLISVRSPRFDLDFAGERSFREAAGRRPPVRGEIAWAGLPTGSIED
                     ATVGGGCGRFEPAAGLVDFERTPGGGIAFPARVNGAELRFALDADADGPPSISAERAA
                     SLSLSVSRDVYGRSIASGATLEVGGFTCRGIHIEVGNAPEGADGVIGGTLFREAVVEL
                     DPKSGRLGLHDPARWVSPAGFNRILVDD"
BASE COUNT          537 a         1343 c         1330 g          630 t
ORIGIN      
        1 acgacggtcc agtagcgcac tccgcagcgc gcgatagact ccgcgcagaa ttcccatgga
       61 cgaggcctcg cgtgcgcgga tccgcgtctt tctgaacgac ccggcggccc gcggagagcg
      121 ccccgtccca tccgacccgc cgcgcgagac cgaacggagc ccgtgggctt tccgcgtcca
      181 gggccgcgag ccgcagcccc gcgagtacga gcccgacacg cccgagtttc tccactggca
      241 gatcacctcg gccctcgcgc gtggccgggc gctgtggtct cgactcctgc cgaaggcggg
      301 ccggtggatc ccgggggacg tgctcgaggc gatcccggtc gacggaaaag acctgaacgc
      361 gttctacgac cggcgcgcgc tgcacttctt tcgcgacgtc gatgagaaga cgggcgccgt
      421 cgtgcagtcg ggcgacagtc ccgacgtcgt cacgcacgag caggggcatg ccgttctcga
      481 ctcgattcgc ccggacctct gggacgcgcc ccacttcgag gtcgccgcct tccacgaggc
      541 gttcggcgac gtcgcggcga tcctcgtcgc gctcgaggag aaggagctcg tcggcgacgt
      601 cctcgaggag acgggcggag cgatcgatca ctcgaatctc gtctcgcggc tcgccgagga
      661 gctgggggcg gcggtcgcgg ccaggtacgg accgggagcg gcgctgccgg acgcgctgcg
      721 cgacgcagtc aacgcttttc gctaccgcgc gccgcgttcc cttccggagg acgctccggc
      781 cggcgatctc tcgcgggagc ctcactcctt ctcccgcgtc gtcacgggcg tctgctggga
      841 cgtcctcgtg cgcctgtatg cgtcaaagag ccgtgtccgt gcggccgccg cgacggaggt
      901 gcgcgtcgac gcgctcggcc tcgccgcgcg agagctcggg cgcctgatcg ccgcagccgc
      961 cgcgaacgct ccgccaggcg ccgctttctt ccggcgtttc gccggcgagc tcgcgtccgc
     1021 agctgccggg gccgcgggcg aacggactga agtcaccgag gcgctcgtct cccgctcgct
     1081 tctcgcgagc gcgccgccgg agccgcgcgg gcgtggcgaa cgcgttgccg ccatggccct
     1141 cggcgccgaa ccgctgccga cgcgggttct ggactcggtc cgcgagcggc tcggagcacc
     1201 gatcgacgag gagatcctcg tccgctccgc gccggatggc acgctgcacg gccggagggc
     1261 gcgcgatctc ttcctgtcgg gcgcggacta tggaccggcg gacggcgccg ccgtcgaaat
     1321 ctcggactcg ttcgcggtcg ccgtggaaga gagcggcttc gtgacagcct cgcgcgtcga
     1381 gcgcgcggga cccgaagacg aagaggacgc gcgcgagttc gtccgcgctc tcgcgcgaac
     1441 cggacggatc gccgaggatg cgtcgctcga ttcgtcggct ctgtatcgcg aaggcaagtc
     1501 gcacgtcgtt cggcgcgagg cggacggcgt gcggcgcctg cgacgcgcct gggttgccgg
     1561 aggacgacga tgagaagaag atcccgcggt ctcgggctcc tcgcggccgc cctgctcgca
     1621 tccgcttgcg cgtcgtcgcg cgaaacctct tctcacccgg cggacgcggc ccgaatctct
     1681 ccggcggagc tcctggcgtc gctcgcggac gtcgcgcagg gccgaaacgc cgatctcgcg
     1741 gaaaccggac tcgtgctgaa gaaggtcgag ctgaaggtcg tcgtcggcca cgaccgccga
     1801 gcgggtgcgc acgcgagctt cctcgtcctg gacgtggaat cgtcgcgacg aagcgaggtc
     1861 tcgttcacgc agaccttcac cttcgacctg ccgcctcccg aacgacggcg atccgcggcg
     1921 ccgccggtcg ccgtccccgg agtcgcggag ttcgtcgacg cggcaatcgc gaccgcccgt
     1981 gagctcgccg ccgtggccgc gcgcgaagac cttccccagc ggctctccga ggtggagctc
     2041 accgcgaaga tcgttcgcgc tcagagagcg gaagggggcg tggcgttcca cgggctggga
     2101 tcggcttccc tcggcggcgg ggtggccggc agctcggagg agtcgaatac cgtgaaactc
     2161 gtcttcggac ggcgctgagt cgaaggcccg gaacgaaaaa gcggtcccga aggaccgctt
     2221 tccgacggca gtgaagcgct cttatttctt ggcgccgagg atcgcgtcct tcgctttctt
     2281 cgacacgcgg aacttgacca cgcgcttcgc ggggatcttg atctcctcgc cggtcgcggg
     2341 attgcggccc atgcgcgcct tgcggttcac caggacgagc ttgccgatgc ctggaatcgt
     2401 gaagctgttc ttcgcttcct tgtacgcgag cgaagtcagg tcatcgagga actgggtcgc
     2461 ggtccgtttg gtttgaccgc ccgcctgtgc gagcttgtcc gcgatctgag acttcgagag
     2521 cgccttgccg gatttttctg ccatatgcac taccccttct tcatgtcgga ttcgcccttg
     2581 cgggccgatc aaatttggcg gctattctag tccgcccgat ggttgactca agtgaaaaaa
     2641 cgcgtgggca cgggtttttt cgtgtatccg tcgccgcagc gcaacgcgca ccgagtggat
     2701 taagatcggt tgcagggact cgaccgaaga attccacgcg cgggaaatcg gcatgacccc
     2761 acgacggcga cgccgcctcg gagagatcgc tgctgtcgtt ctcctcgtga cgccactcgc
     2821 gtgtcgcctc gacggcgacc gcaaggtcgc cgggagaatt ctcgatcggc accggagagc
     2881 gacaggcgcg aagccgcttc cggcctcgca cgtcatccgg atgcgccttt catccgagac
     2941 gcccggcggc gggacggggg tcgccgaggt cgcgtgggag ccgaaccggt tccgggagcg
     3001 catcgcttcg gccggtgcaa cgaccgagcg cggcatccag gggggaaagg cgtacttcgt
     3061 cgacgaggac ggcgtcacgc gcgtcggctc ggaacccgtc cttcgcgagc tgctcgcccg
     3121 ctcctatttc tggcggagag cctggctgtt cgccgatcag ggcggcgcgc gcctctccct
     3181 ggggccggcg gacccctcct cggtctcggt ccgactgcag cccttcggct cgaatcccct
     3241 cctcctgtcc ttctcccggc gggacgggcg cctgatttcc gtccgttcgc cccgtttcga
     3301 cctcgatttc gcgggcgagc ggtcttttcg ggaagcggcg ggacggaggc cgcccgtccg
     3361 cggcgagatc gcctgggccg gcctgcccac gggaagcatc gaggacgcca cggtaggcgg
     3421 tggatgtggc cggttcgagc ccgccgcagg gctcgtcgac ttcgagcgga cccccggcgg
     3481 aggaatcgcc tttcccgcac gggtcaacgg cgccgagctt cgcttcgcac tcgacgccga
     3541 cgccgacggc cccccttcga tctcggcaga gagagcggcc agcctgtcgc ttagcgtctc
     3601 gcgggacgtc tacgggcgtt cgatcgcctc cggcgcgaca ctcgaggtcg ggggcttcac
     3661 ctgtcggggc atccacatcg aggtcggcaa cgcgccggaa ggcgcggacg gcgtcattgg
     3721 cggcaccctc tttcgcgaag ccgtggtcga gctcgacccg aagtccggca ggctcggcct
     3781 tcacgacccc gcacggtggg tctctccggc cgggttcaac cgcatcctcg tcgacgacga
//
LOCUS       DASWAV010000664         2082 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U2665
            k141_438018, whole genome shotgun sequence.
ACCESSION   DASWAV010000664 DASWAV010000000
VERSION     DASWAV010000664.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960316
            Sequence Read Archive: SRR10260007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2082)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2082)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:47:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,929
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2082
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_438018"
                     /isolate="SMAG_U2665"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.36444 N 132.31889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1426)
                     /locus_tag="VF958_14005"
     CDS             complement(<1..1426)
                     /locus_tag="VF958_14005"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HEX9150269.1"
                     /translation="MSLAVGSRLGPYEVLAPLGAGGMGEVYRALDPRLGRDVAIKVVH
                     PRLAADPDRLSRFEKEAHAAARLDHPNILVVHDVGTHEGSPFIVSELLQGESLREKLG
                     APLGPKKAVEYAIQIAHGLAAAHEKGIVHRDIKPENIFVTKDGHVKILDFGVAKLLPT
                     FEASGADTQTPTEIATQPGTALGTVAYMSPEQIRGQPVDARSDLFSLGIVLYEMLSGK
                     RPFQRGTVADTISAILHEEPPSLADSKTEIPPALERIVTQCLEKQLGDRLSSAHDLAL
                     ALRSISDQQPTGRSAESPRRRRRLWLSTLVVVAAALAAVLVLKLPRNISRNTPEEKNS
                     APPRQITSAPGWDDEPALSPDGTLVAYSSDAAGSSDIWVVDVQGGEPMRLTDGTGEES
                     KPTWFPDGRSVAYASIRAGKKSVWRVARLGGSPSLLVEDAETPAISPDGTRIAFARPG
                     PVGSLRIWTAPLDHPADARRLTGDD"
     gene            complement(1624..1698)
                     /locus_tag="VF958_14010"
     tRNA            complement(1624..1698)
                     /locus_tag="VF958_14010"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1663..1665),aa:Glu,seq:ttc)
     gene            1765..>2082
                     /locus_tag="VF958_14015"
     CDS             1765..>2082
                     /locus_tag="VF958_14015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9150270.1"
                     /translation="MFGNSPAESGRSLDRRIAQLIDGGRSSAEPPFYSTEDAASDVLV
                     TRLRHQGIEAELEQESGNWYCVFWASQQGDAVRERVASGSAAARALAICRAVVNLHLS
                     GTGE"
BASE COUNT          343 a          668 c          715 g          356 t
ORIGIN      
        1 cgtcgtcgcc tgtgagtctc ctggcgtcag ccgggtgatc caatggagcc gtccagattc
       61 ggagcgagcc gacgggccct ggacgtgcga aggcgattcg tgtgccgtcc ggcgagatcg
      121 ccggcgtctc agcgtcctct acgaggagtg acgggcttcc tcccagccgg gcgaccctcc
      181 acacggattt cttgccggct cgaatcgagg catacgcgac cgagcgtccg tcggggaacc
      241 aggtgggctt gctttcctcg cctgtaccgt cggtgagccg catgggctct cccccctgga
      301 cgtccacgac ccagatgtct gagctccccg ccgcgtcgga actgtaggcc accagtgttc
      361 catcgggaga gagcgcgggc tcgtcgtccc atcccggtgc gctggtgatc tgtcgcggcg
      421 gggccgagtt cttctcctcg ggcgtgttcc tcgagatgtt tcgcggcaac ttgagaacca
      481 ggacggccgc gagcgcggcg gcaaccacga caagtgtcga gagccacagt cggcgccggc
      541 gtcgcggact ctctgccgac ctgccggtgg gctgctggtc cgagatgctg cggagggcga
      601 gcgcgaggtc gtgggccgag gagagccgat cccccagctg cttttccagg cactgggtga
      661 cgatcctctc gagcgcggga gggatctccg tcttgctgtc cgcgagcgag ggcggttcct
      721 catgcaggat ggccgaaatc gtgtcggcga ccgtacctcg ctggaagggg cgcttgcccg
      781 acagcatttc gtacaggacg atgcccagcg agaagagatc cgaccgagcg tccacgggct
      841 ggccgcggat ctgctcgggg gacatgtagg cgacggtgcc caaggcagta ccgggctgcg
      901 tggcgatctc cgtgggcgtc tgcgtatctg cgccggaggc ttcgaaggtc ggaagcagct
      961 tggcgacgcc aaagtcgagg atcttgacgt gcccgtcctt cgtgacgaag atgttctcgg
     1021 gcttgatgtc gcggtggacg atccccttct cgtgcgccgc ggcaaggccg tgcgcgatct
     1081 ggatggcgta ctcgacggcc tttttcggcc ccaacggggc gccgagcttt tcgcgcagcg
     1141 attcgccctg cagaagctcc gagacgatga acgggcttcc ttcgtgcgtg ccgacgtcgt
     1201 ggacgacgag gatgttgggg tggtcgagcc gggcggcggc gtgggcttcc ttctcgaagc
     1261 gtgagaggcg gtcgggatcc gcggcgaggc gagggtgaac gaccttgatg gcgacatccc
     1321 gcccgagccg tgggtcgagc gccctgtaca cctcccccat tccacccgcg ccgagaggcg
     1381 cgaggacttc gtagggaccc agacgggacc caactgccag ggacacggct agacgcagct
     1441 aggacacggc attgacgacg tcgcaagatg caattgaatc cccccccccc gccgccgagc
     1501 ccgccgccca gaggaacaag gattgtgagg tccgatacgg gtcccggcga acgcctccgc
     1561 gacgtacaag ccagccgtgt cgaggacacg gcgattggag gagggagctc tacacgcgaa
     1621 tgctggtggc cccaacggga ctcgaacccg tgccgcggcc ttgaaagagc cgtgtcctaa
     1681 ccactagacg atggggcccg aaacgaaaat agcacagcgt tcccgatccg gccggtttcc
     1741 ccaagcgtag aattctggct tccaatgttc ggcaactctc ccgcggagtc gggtcggtct
     1801 ctcgatcggc ggatcgccca gctgatcgac ggtggtcgaa gcagcgccga gccacctttc
     1861 tattcgaccg aggacgccgc ctccgatgtt ctcgtcaccc ggctccgcca ccagggcatc
     1921 gaggctgagc tcgagcagga gagcggaaac tggtactgcg tcttctgggc ttcgcaacag
     1981 ggcgacgccg tccgggagag agttgcgagc ggaagcgcag cggcgcgcgc cctggcgatc
     2041 tgccgcgccg tcgtcaacct tcatctttcc ggaaccggcg aa
//
LOCUS       DASWAV010000665         2267 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U2665
            k141_280086, whole genome shotgun sequence.
ACCESSION   DASWAV010000665 DASWAV010000000
VERSION     DASWAV010000665.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960316
            Sequence Read Archive: SRR10260007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2267)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2267)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:47:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,929
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2267
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_280086"
                     /isolate="SMAG_U2665"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.36444 N 132.31889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1769)
                     /gene="leuS"
                     /locus_tag="VF958_14020"
     CDS             complement(<1..1769)
                     /gene="leuS"
                     /locus_tag="VF958_14020"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR00396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004823 - leucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006429 - leucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HEX9150271.1"
                     /translation="MTERYDPAEIEQKWQRRWAEERVAEVDTGVPGNEFYMLNMYPYP
                     SASRLHVGHGRNYILGDALYRRERMAGRKALNPMGWDAFGLPAENAAIQSGVHPREYT
                     LANIARMKEQLYSLGLLYDWSKELASCDPGYYRWNQWLFLRMWEHGLAYRKTAPVNWC
                     PGCRTVLANEQVVNGRCERSDDPVEIRDLTQWFFRVTDYAERLLAGLDRLDDWPERVK
                     TMQRNWIGRSEGAEIFFRVEALTEPIAVFTTRADTLFGATFLALAPEHPAVAVLAERS
                     DRRDAIGDFVEKVRRESRLDREAEGGVKEGLDTGFVALNPATGEQIPVWLANFVLPEY
                     GTGAIMGVPAHDQRDFEFARRFRLPIRPVYRTEERELDPDTMTEAMPHVGFLCNSEDW
                     NGTPCGPDAVRLAVEWIEAKGLGKGKTGYRLRDWLISRQRYWGTPIPAIHCEKCGVVP
                     VRDEDLPVLLPDDVEFRGAEGNPLEKSQRFVETRCPKCGGPARRETDTMDTFVDSSWY
                     YLRFLNPHDQERMVDPERVSRWMPVDQYVGGIEHAILHLLYSRFVCRVLKDFGLVDLE
                     EPFERLFNQGMITKLNPVTGKVE"
     gene            complement(1766..>2267)
                     /locus_tag="VF958_14025"
     CDS             complement(1766..>2267)
                     /locus_tag="VF958_14025"
                     /inference="COORDINATES: protein motif:HMM:NF013961.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transglutaminase-like domain-containing protein"
                     /protein_id="HEX9150272.1"
                     /translation="RLASGATTQADAVERILSGLAAFVRYDPDRVRRQEPSAVFAERH
                     ANCVGLSELAVDLLRRIGISARTVQGVLKTEPGSAAYDAAIGGAYHRWIEVHYPDRGY
                     VFSDPSASVNGVDARYLPFSRRAYVRPRELRVIPLALTGKLEYRSISAGSAHLRARAT
                     AIFPAP"
BASE COUNT          378 a          785 c          735 g          369 t
ORIGIN      
        1 ttttcgacct tgccggtcac cgggttcaac ttcgtgatca ttccctggtt gaagagacgc
       61 tcgaagggct cctcgagatc cacgaggccg aagtccttca ggacgcggca gacgaaccgc
      121 gaatacagca ggtggaggat cgcgtgctcg atcccgccga cgtactggtc gaccggcatc
      181 caccgcgaaa cgcgctcggg gtcgaccatc cgctcctggt cgtgcgggtt cagaaacctc
      241 aagtagtacc aggacgagtc gacgaacgtg tccatcgtgt ccgtctcgcg ccgcgcgggg
      301 ccgccgcatt tcggacaacg ggtttccacg aaccgctgcg acttctcgag agggtttccc
      361 tcggcgccgc gaaactcgac gtcgtcgggc aggagcaccg gaaggtcttc gtccctcacc
      421 ggaaccactc cgcacttctc gcagtggatc gccgggatcg gcgtgcccca gtaccgctga
      481 cgcgagatga gccagtcgcg caggcggtag ccggttttcc ccttccccag acccttcgcc
      541 tcgatccact cgaccgcgag gcgcactgcg tccgggccgc agggcgtccc gttccagtcc
      601 tccgagttgc agaggaagcc gacgtgcggc atggcctcgg tcatcgtgtc gggatcgagc
      661 tcgcgctcct cggtccgata gacgggccgg atcggaagcc tgaagcgccg cgcgaactcg
      721 aagtcgcgct gatcgtgcgc cggtacgccc atgatggcgc cggtgccata ctccgggagg
      781 acgaagttgg cgagccacac cgggatctgt tccccggtcg cgggattcaa cgcgacgaag
      841 ccggtgtcga gaccctcctt gaccccgccc tccgcttcgc gatcgagccg cgactcccgc
      901 cgcaccttct ccacgaaatc cccgatggcg tcgcggcggt ccgatcgctc cgcgagcacg
      961 gcgacggccg gatgctccgg cgcgagagcg aggaacgtgg ccccgaagag cgtgtctgcc
     1021 cgcgtcgtga agacggcgat cggctccgtg agcgcctcga cccggaagaa aatctccgcc
     1081 ccttccgacc ggccgatcca gttgcgctgc atcgtcttga cgcgctccgg ccagtcgtcc
     1141 aggcgatcga gcccggcgag gaggcgctcg gcgtagtccg tcacacggaa aaaccactga
     1201 gtcaggtcgc ggatctcgac gggatcgtcg gaccgctcgc accggccgtt gacgacctgc
     1261 tcgttggcga gcaccgttcg acacccgggg caccagttca cgggcgcggt cttccggtag
     1321 gcgagcccgt gctcccacat gcggaggaag agccactggt tccaccggta gtaaccgggg
     1381 tcgcacgagg cgagctcctt ggaccagtcg tacagaaggc cgagcgaata cagctgctcc
     1441 ttcatgcgcg cgatgttcgc gagcgtgtac tccctcggat ggactccgct ctggatcgcg
     1501 gcgttctcgg ccggcagtcc gaacgcgtcc catcccatcg ggttcaacgc cttgcggccc
     1561 gccatccgct cccggcggta cagcgcgtcg ccgaggatgt agttgcgccc gtggccgacg
     1621 tgcaggcggc tcgccgacgg atacgggtac atattcagca tgtagaactc gtttcccggc
     1681 acgccggtat cgacctcggc gacgcgctcc tccgcccagc gccgctgcca cttctgctcg
     1741 atctcagccg ggtcgtaacg ttcggtcatg gggcggggaa tatagcagtc gccctcgccc
     1801 gaagatgggc ggaacccgcg gagatgctcc ggtattcgag ctttcccgtc aacgccagcg
     1861 ggatcactcg cagttcccgg ggccggacgt acgcccgccg cgaaaacggg aggtaacgtg
     1921 cgtccacgcc gttgaccgaa gcgctcggat cggagaaaac gtagccgcgg tcaggatagt
     1981 ggacctcgat ccatcggtgg taggcgccgc cgatcgctgc gtcataggcg gcagagccgg
     2041 gctcggtctt caacaccccc tggacggtgc gcgccgaaat gccgattcga cgcagcaggt
     2101 cgaccgcgag ctcggaaaga cccacgcagt tcgcgtgccg ctcggcgaag accgcagatg
     2161 gctcctggcg ccgcacgcgg tccgggtcgt accgcacgaa cgcggcgagc cccgaaagaa
     2221 tccgttcgac ggcgtccgcc tgggtggtcg ctcccgacgc caggcgc
//
LOCUS       DASWAV010000666         9846 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U2665
            k141_559059, whole genome shotgun sequence.
ACCESSION   DASWAV010000666 DASWAV010000000
VERSION     DASWAV010000666.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960316
            Sequence Read Archive: SRR10260007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 9846)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9846)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:47:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,929
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9846
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_559059"
                     /isolate="SMAG_U2665"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.36444 N 132.31889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..452)
                     /gene="tilS"
                     /locus_tag="VF958_14030"
     CDS             complement(<1..452)
                     /gene="tilS"
                     /locus_tag="VF958_14030"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: protein motif:HMM:NF013348.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HEX9150273.1"
                     /translation="MDALRTALLGAADRGLMPRGASILLAVSGGADSTALLHATAELA
                     PETRWTLSVAHVHHGWRGRDADRDLAFVAGHAGRLGLPFFHRRRDARGEARLFGLSPE
                     AGARRVRYEALLEMALEAQASRIATAHQRDDRVETHLLARRRKGGRASL"
     gene            complement(461..724)
                     /locus_tag="VF958_14035"
     CDS             complement(461..724)
                     /locus_tag="VF958_14035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9150274.1"
                     /translation="MKPVRRRIGASTIRVLSATDSCRDRLAAFYHWNDRQSLDAAVRI
                     ALRNRVDMPKIRTWSAQEGAADGFLEFSRELARARSRKPGPAS"
     gene            complement(1455..1531)
                     /locus_tag="VF958_14040"
     tRNA            complement(1455..1531)
                     /locus_tag="VF958_14040"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1495..1497),aa:Met,seq:cat)
     gene            1797..3863
                     /locus_tag="VF958_14045"
     CDS             1797..3863
                     /locus_tag="VF958_14045"
                     /inference="COORDINATES: protein motif:HMM:NF012861.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005247 - voltage-gated chloride channel
                     activity [Evidence IEA];
                     GO_process: GO:0006821 - chloride transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chloride channel protein"
                     /protein_id="HEX9150275.1"
                     /translation="MSSPEPSSPEPPVPSRPSRLGRFFWRDPNTQFLLLAAAVGVAGA
                     LGAIGFRAISRVLTTLLFDAEDVVAGAESLPPLLRIFLPAIGGLTGGLIIQAFAKSGG
                     PHGISQMIEVVHLGRRAVRMRPSLGRSAASLAVISTGGSEGREGAIIQMGAAFASWLS
                     RKVKVSPERARILVACGMAAGVAGAYNTPIAATLFVLEVVVGSFSMTLFGPAVVSAAI
                     STVLARYFLGDEPVYQVAAFRLESLLEFLPFALIGVGAGAASALFVRTLRASKKVFSS
                     SRLALPLSMAIGGAIVGTIGIVRPEVWGNGFDGTNRILRGNPTILLLAELFVGKIAAT
                     AATIGSGGVGGVFTPSLMVGAALGGSVAKLVQAVLPILEAPVGGYALLGMGGLLAGVT
                     RAPLLAIIMIFELTQNTAVLVPMMAVSVLAVLTARAFQKESHYIEGLRSAGIVWEKTP
                     EATALSSLTVADVMREDIKLVPRSTPLPEIVQAFLHSRSLYLYIGDEEGRLLGVIDLH
                     DIKETLPEREISNLVIAEDLLQEIPFVTRSEPLTSVNEKLWFRDLGQLPVVDSAENRR
                     FLGIVTRRDLLGAFDREVLLHNRLVARVRMFREGGSEMDYFELPEKHRLVQVDVPPAI
                     EGRTVAESGLRNRYGVSVLAVKRLSRGGLERRFVPGPEDRLQHGDVLIVLGTDEAVAR
                     LKAGAM"
     gene            3931..5250
                     /locus_tag="VF958_14050"
     CDS             3931..5250
                     /locus_tag="VF958_14050"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HEX9150276.1"
                     /translation="MTRSTGASELVALRGKWQKQRKRLYDRETGLPTVAALMEELVAQ
                     LRENGSLSILSFCPGSEGRVEEVWGWKAYDDLLLDFVRRLKAFQSDGILPAGTFCLPH
                     VSSDEVLLVIDPAGGRDRIPALEQKAAELDLFIRGYLAERADLPGRFRSFVGSSRVHA
                     DPRRRAERLIYSGVRDARDRVTRQTIRAEIRGAAVLQEAINRRDFTPVFQPVFDLSTG
                     DMIGMEALSRGPKGSDLESGETLFSLAERTELLGPLERVCRDLALEAVSPNESGQKLF
                     MNLSPAAASDPEFLGPQFRDNLASLGFQPESIVLEITERTYVVYESLFRDVLTRFREQ
                     NFGIAVDDVGTGYSSLASLADIQPDYLKFDHVFVRDLDRRQIKQDLLEALMSFARKMN
                     TRVIAEGIETREELEALRGLGVEYGQGFLLASPMAIEEIPRYYRVAV"
     gene            5270..5344
                     /locus_tag="VF958_14055"
     tRNA            5270..5344
                     /locus_tag="VF958_14055"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5302..5304,aa:Val,seq:tac)
     gene            5541..6908
                     /locus_tag="VF958_14060"
     CDS             5541..6908
                     /locus_tag="VF958_14060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9150277.1"
                     /translation="MTRSPRSERPRSPEGRRLERSIDFLSITAIAAHVFLVLSLRVTA
                     WPEVTTPAYLWSRGMLLYRDIKFQHGPGTIGTLALAFLAFGARTWVVRAYAIVWPLLA
                     HVCVLRFTRSFSLAERALGSAFFLTLFFSLDGHAVWPTVVMAAVSLPTAAALSRNQAF
                     RAGLLIGLSILFKQTAAYVLLFASIGLALERRWRSAAVVALVSCIPYWAAAAVFGLLG
                     AGPEMLRWTLEVPLTIRPAFVTARPSAFDASVLVFAFLPTVTEALLERPGEYEVRSRW
                     LLLVAIGFAAICYPRFTLSQTVAAIPCLAVGAARFLRRRAPLPIFRRAAYVLVIALIV
                     GRLLVLASGGDFDGKVLYWNHEPSLDALAERLRRFPPDTPLFSPLWDNVLPRAGLIPP
                     GRLYVNPWFDWFFPVDDVGRRIQEAIRRRGALVVGYRAARADGEVVGPYTIRWVKPDP
                     DRPGR"
     gene            7084..7160
                     /locus_tag="VF958_14065"
     tRNA            7084..7160
                     /locus_tag="VF958_14065"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7118..7120,aa:Asp,seq:gtc)
     gene            complement(7168..8937)
                     /locus_tag="VF958_14070"
     CDS             complement(7168..8937)
                     /locus_tag="VF958_14070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9150278.1"
                     /translation="MTYLRKRTVLSCLACAYSTTLLAQSPRIPVTNWTVPAYRGAVAG
                     GVSPMSDISPGVGFVAVTPCRLVDTRGGEPFTGAYGPPALVAHATRDFDLNSAPHCPG
                     IPAGVDAYSLNLGAILPAADGFLSAWPTGSAMPTVSAVNYLAGEVIANAEVVPTGTGG
                     SISLTANTNTDVYIDINGYFTDSYNDGVPFRAIGNVPGGPSPGSGVVLAWNTNTTNSY
                     SNGGAFFSEACHTGSAGVVGETLSSGAPGCGPVIGVWGISENDFDASTGVYGVQWAPT
                     GIVVGATNSSSNDSAGVWGYAGGLAPGRNYGITGESNSVVNGAAGVLGIDVNGMTVSP
                     SGPGGTIGVRGDSRFSTGVLGLSEDHDAGQRAVTGQLLNFGNGTPIASGHLGYDAGMN
                     NYAVFAAGDYGGTGAKYFVEPHPTDASKVIRYIALEGPEAGTYFRGKGRFQNGVATIA
                     VPEDFRLVTDPDSLSVVATPIGDMAVIAVARVGLDRIVLRSSRNVEFFYLVNGVRKTH
                     KDLAPIVAGNEYAPASADARMPPSLTDGQRQMLVSNGTYNADGSVNLETARRLGWDRI
                     WEERARPRPEPARAKSLPGLADR"
     gene            complement(9024..>9846)
                     /locus_tag="VF958_14075"
     CDS             complement(9024..>9846)
                     /locus_tag="VF958_14075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9150279.1"
                     /translation="PAPSLTCAGATGLSIAIQVCAGPGGAPGGFAIQWTTAESLAAGP
                     DGLPGTADDSTWPASDDADLCTASFVGRARFKKPELGGGECTTVEVGQLLGDSGAPTT
                     CAGSLAGGSSYVFRALARGAAAPIALSVSPDLLCGTLACTPAVAGCVVTAAYWKDHGP
                     LPRRGNADEWSVSSVLLGNVTYSDVDLQAILETPADGNGLIALAHQLIAAKLNIANGT
                     DSIVVASFVAEADKVIGDLVVPPGGSGYLELAATWDLTQALTGYNEGALGPGHCD"
BASE COUNT         1489 a         3327 c         3256 g         1774 t
ORIGIN      
        1 agactggcac ggccgccctt gcgccgcctg gcgaggaggt gcgtctcgac cctatcgtct
       61 cgctggtggg ccgtcgcgat gcgcgaggcc tgcgcctcca gcgccatctc gaggagcgcc
      121 tcgtaacgga cgcgccttgc cccggcttcg ggggagaggc cgaagaggcg cgcttctccc
      181 cgcgcgtccc gccgccgatg gaagaagggg agcccgagcc ggcccgcgtg cccggccacg
      241 aacgcgaggt cgcggtcggc gtctctcccg cgccagccgt ggtgcacgtg cgcgacggag
      301 agcgtccagc gggtctcggg cgcgagctcg gccgtggcgt ggaggagcgc cgtcgagtcg
      361 gctccgccgg aaaccgcgag gaggatcgac gcgccacggg gcatgaggcc gcggtcggcc
      421 gcgccgagca atgccgtacg gagcgcgtcc attccttatg ttacgacgcg gggcccggct
      481 tccgagacct ggctcgcgcg agctcacggg aaaactccag aaaaccgtcg gcagcgcctt
      541 cctgggcgct ccaggtccgg atcttcggca tgtcgacgcg gtttcgaagc gcgatccgaa
      601 ccgccgcgtc caggctctgc cgatcattcc agtggtagaa ggccgcgagc cgatcgcggc
      661 aggagtcggt ggccgaaagg acccggatcg tcgaggcgcc gatccggcgc cgtactggtt
      721 tcactgcgag atcccgtccg acccccagtg gaccactcgg aaattcgacg aagaacctcg
      781 tcttcgcgtg gaagtattgg tcgcggcgcc ggcggaatcc cacgctcgcc attgcctcgt
      841 cgagttctcg ctgagagggc gcggactcga gaatgaaatc gacgtcttcg gagtgatacc
      901 cgccggccgt gtggatcgcc gcacaggcgc ctccggtgag aaccgctcga atgccggtct
      961 ttctcagggc ttcagcgacg gcgatcgcga ccgtctcgag gctcgacccg gcggtgagtc
     1021 tcagaggcgc ttcccgggct ttcgcggtcg cctgcgaatc cggccgatgc gtgctcggag
     1081 atcgtcttcg gcttccgcca gacgcgcgag atactttcga agctcgtcgt aggcaaagta
     1141 gcgaggcgac agcctgagaa gccgggttcg gccccgggcg cgtcccgcga tcaggccgtc
     1201 cgactccagg cctttcagcg ctttctgaac gccggagagc ggtcgctcca gcaaccgggc
     1261 gatttcgcgc gcgtaactct catcgagtaa acccagggcg atgagcgcct ccgttctcgt
     1321 gcggcctccg aaaggcgacg atcgtccgac caccataagt agatcatgag accacttata
     1381 gcggtcattg tcaaatccat gaagagcaac ggcgcagaca cgcgtcccga gaacggcgtc
     1441 ttcctcaggg aacttggtgg cggtgcaggg acttgaaccc cggacacagc ggatatgagc
     1501 cgcttgctct aaccaactga gctacaccgc cgctatgaag gaccggggat gctagcacag
     1561 cccgccgcct gatcccccga ccgtatactc gttttcgtgc cagcccccga tcggtgcgag
     1621 gtctgtctgc agactttcgg attcagcctg ccgcggtgtc ccctctgcca ccgcactttc
     1681 tgcgattcct gcgcgatgcg gagaggtggg ctcgtcttct gcggtgcggg atgcgcccac
     1741 ctcttcttct tcggcgaaga ggacgaggaa gaccttcccg aatcggaatc cgacgagtga
     1801 gctctcccga gccgagctcg cccgaacctc cggtcccgtc ccggccgtcg cggctgggac
     1861 gtttcttctg gcgcgatccc aacactcaat tcctcctgct ggcggcggcg gtcggggtcg
     1921 cgggcgcgct cggcgcgatc ggattccgcg cgatctcccg ggtgctcacg acgctccttt
     1981 tcgacgccga ggacgtcgtg gcgggcgcgg aatcgctccc accgctgctg cggatcttcc
     2041 tgccggcgat cggtggtctc acggggggcc tgatcatcca ggcgttcgcg aagtccgggg
     2101 gacctcacgg gatctcccag atgatcgaag tggtccatct cggccggcgt gcggtccgga
     2161 tgcgcccttc gctcggccgg tcggcggctt cgctggccgt gatctcgacc ggcggatcgg
     2221 aaggacgcga gggcgcgatc atccagatgg gcgccgcgtt cgcgtcgtgg ctttcgcgaa
     2281 aggtcaaggt ctcgcccgag cgcgcgcgga tcctcgtcgc gtgcggcatg gccgccggcg
     2341 tcgccggtgc ctacaacacg ccgatcgcgg cgactctctt cgtgctggag gtcgtcgtcg
     2401 ggtccttctc gatgacgctc ttcggcccgg cggtcgtctc cgccgcgatc tcgacggtcc
     2461 tcgcccgcta ctttctcggc gacgaacccg tctatcaggt ggccgcgttc cgcctggagt
     2521 ctctcctcga gttcctcccg ttcgcgctca tcggcgtcgg agcgggcgcc gcgtccgccc
     2581 tcttcgtgcg aaccctccgc gcgtcgaaaa aggtcttctc gtcctcccgc ctggcgctgc
     2641 cgctctcgat ggcgatcggc ggagcgatcg tgggcaccat cggaatcgtc cgtccggagg
     2701 tctggggcaa cggcttcgac gggaccaatc gaatcctccg cggcaatccg acgatcctcc
     2761 tgctcgcgga gctcttcgtc ggaaagatcg cggcgacagc ggcaacgatc ggttcgggag
     2821 gagtcggagg agtcttcacg ccgagcctga tggtcggcgc ggctttggga ggctcggtcg
     2881 cgaagctcgt gcaggccgtc ctgcccattc tggaggcgcc cgtgggaggc tacgcgctcc
     2941 tcggaatggg aggactcctc gccggcgtca cgcgggcgcc gcttctcgcg atcatcatga
     3001 tcttcgagct gacacagaac accgcggtcc tcgttccgat gatggcggtt tccgtgctcg
     3061 cggtcctgac cgcgcgcgcg tttcagaagg agtcgcacta catcgagggc ctgcgctccg
     3121 ccgggatcgt ctgggagaag acgcccgagg cgaccgcgct gtcatcgctc acggtcgccg
     3181 acgtcatgcg cgaggacatc aagctcgtcc cgcgctcgac gccgctcccc gagatcgttc
     3241 aggcttttct ccactcgcgg agcctgtacc tgtacatcgg cgacgaggag gggaggctgc
     3301 tgggcgtcat cgacctccac gacatcaagg aaacgcttcc cgagcgcgag atctcgaacc
     3361 tggtcatcgc ggaagacctc ctccaggaga tccccttcgt gacccgctcc gagccgctga
     3421 cgagcgtcaa cgaaaaactc tggttccgcg acctcggcca gctcccggtc gtcgattccg
     3481 cggagaaccg ccggtttctc gggatcgtca cgcgccgcga tctcctcggc gcgttcgacc
     3541 gggaggtgct cctccacaac cgtctcgtcg cgcgcgtgcg gatgttccgc gaagggggga
     3601 gcgagatgga ctacttcgag ctgccggaaa agcaccgcct cgtgcaggtg gacgtacccc
     3661 cggccatcga gggtcggacg gtcgccgagt cggggctccg caaccgctac ggcgtctcgg
     3721 tcctcgcggt gaaacgcctc tcgcgcggag ggctcgagcg gcgcttcgtg cccggcccgg
     3781 aagaccgcct gcagcacggt gacgtgctca tcgtgctcgg cacggacgag gccgtggcgc
     3841 gactgaaggc tggagcgatg tgacgaccga cacacggcct gcggtgaccc tcgatacggt
     3901 ttttcgcgcc tcgattgcta aaatcgacag gtgacgagga gtaccggcgc gagcgagctc
     3961 gtcgctctgc gcggcaagtg gcagaagcag cgcaagcgcc tctacgaccg ggaaaccggg
     4021 ctccccacgg tcgccgcgct catggaggag ctcgtcgcgc agctccgcga aaacggctcg
     4081 ctctccatcc tgtcgttctg cccgggctcc gaggggcgcg tcgaagaggt ctgggggtgg
     4141 aaggcgtacg acgacctgct cctcgacttc gtgcggcgct tgaaagcctt ccagtcggac
     4201 gggatcctgc cggccgggac cttctgtctg ccgcacgtct cgtccgacga ggtgctgctc
     4261 gtgatcgatc cggccggggg gcgcgaccgg attcccgcac tcgaacagaa ggcggccgag
     4321 ctcgatctct tcatccgcgg atatctcgca gagcgcgccg acctgccggg gcgtttccgg
     4381 tccttcgtcg ggtcctcccg ggtccacgcc gatccgaggc gccgggccga gcggctcatc
     4441 tacagcggcg ttcgcgacgc tcgcgaccgc gttacccgcc agacgatccg ggccgagatc
     4501 cgcggcgcgg cggtgctcca ggaagcgatc aaccgccgcg acttcacgcc ggtcttccag
     4561 ccggtcttcg acctgtcgac cggggacatg atcgggatgg aggcgctctc gcgagggccc
     4621 aagggcagcg acctcgagtc gggcgagacg cttttctcgc tcgccgagcg caccgagctc
     4681 ctcgggccgc tcgagcgcgt ctgccgcgac cttgcgctcg aggcggtttc acccaacgag
     4741 agcggacaaa agctcttcat gaacctctcg ccagcggcgg cgtccgaccc cgagttcctc
     4801 gggccccaat tccgcgacaa tctcgcttcg ctgggattcc agcccgagtc gatcgttctc
     4861 gagatcaccg agcggacgta cgtcgtctac gagagcctct tccgcgacgt cctcacacgg
     4921 tttcgagagc agaatttcgg aatcgcggtc gacgacgtcg ggacgggcta cagcagcctc
     4981 gcctccctcg cggacatcca gccggactac ctgaagttcg accacgtgtt cgtgcgcgac
     5041 ctcgatcggc ggcagatcaa gcaggacctg ctcgaggcgc tcatgagctt cgcgcgcaag
     5101 atgaacaccc gcgtgatcgc ggagggaatc gagacacgcg aagagctcga ggcgcttcga
     5161 gggctcggcg tcgagtacgg acagggcttc ctgctcgctt cgccgatggc gatcgaggag
     5221 atcccccgtt actatcgcgt cgcggtttga gttctcggcg cgtctcgacg gggggttagc
     5281 tcagcggtag agcgcctgct ttacacgcag gaagtcgcag gttcgagacc tgcacccccc
     5341 accaacccga gaaacaaccg actcgaacgt ccggcttctc ccgccagcga gtcttctttt
     5401 cgggcttctc ttcgaacgtc gatttttcga gcggagaaag gattttcctt gaatcgtgcg
     5461 cgtgggaccg caacgatcag gggaagccgt ttgaaacctt tctcgatcga ttcctcgcag
     5521 gttcaccgag aggcggtcac gtgacccggt cgccgcggag cgaacgtccg cgttctcccg
     5581 agggtcgaag gctcgaaaga tcgatcgatt tcctttccat cacggcgatc gcggctcacg
     5641 tcttcctcgt gctgtcgctt cgcgttacgg cgtggcccga ggtgacgacc cccgcctatc
     5701 tctggtcccg ggggatgctt ctctaccgcg atatcaagtt ccagcatggt ccggggacca
     5761 tcgggacgct cgctctcgcc ttcctcgctt tcggcgctcg gacctgggtg gtccgcgcct
     5821 acgccatcgt ctggcctctc ctcgcgcacg tgtgcgtact gcgtttcacg cggtcgttct
     5881 cgctcgcgga gcgtgctctc gggtccgctt tctttctgac gctcttcttc agtctggacg
     5941 gacacgcggt ctggccgacg gtggtcatgg cggcggtgtc tcttccgacc gccgcggctt
     6001 tgagccgcaa tcaggcgttt cgcgccggtc ttctcatcgg gctctcgatc ctcttcaagc
     6061 agacggccgc gtacgtgctt ctcttcgcgt ccatcggcct cgcgctcgag aggcgatggc
     6121 gctccgcggc ggtggtcgct ctcgtgagct gcatcccgta ctgggccgcg gccgcggtct
     6181 tcggcttgct cggcgcgggt ccggagatgc tccggtggac gctggaagtc ccattgacga
     6241 tccgcccggc gttcgtcacg gcacgcccga gcgccttcga tgcgtccgtc ctcgtgttcg
     6301 cgtttctccc gaccgtcacc gaggctcttc tcgagcgccc cggcgagtac gaagtccggt
     6361 cccggtggct tctcctcgtg gccatcgggt tcgccgcgat ctgctacccc cgtttcaccc
     6421 tctcccagac ggtcgctgca attccctgtc tggctgtcgg ggccgccagg ttcctccgcc
     6481 gtcgggcgcc ccttccgata ttccgaaggg cggcgtacgt cctggtcatc gcactcatcg
     6541 tcgggcggct tctcgtcctg gcgagtggag gggatttcga cggaaaggtt ctctactgga
     6601 accatgagcc ctctctcgac gcgctcgctg agagacttcg ccggtttccc cccgacactc
     6661 cgctcttttc gccgctgtgg gacaacgtgt tgcctcgagc gggactcatt cctcccggcc
     6721 gtctctacgt caatccgtgg ttcgactggt tctttcccgt cgacgacgtc ggccgacgga
     6781 tccaggaggc gatccgccgc cgcggcgccc tcgtcgtcgg ctaccgagct gcccgagcgg
     6841 acggcgaggt ggtcggcccg tacacgatcc ggtgggtgaa accggatccc gatcggccgg
     6901 gtcggtagga tccgcggcgg tcggggggtg aagacggtga gtcgaaccga cgccatcgga
     6961 gcgctctcgg gtgagaaccg cacctttccg ccatcggaga agttgcaggg aaggcgaacc
     7021 tccgcgggct tccgcgcctt gcttgtgatc caccttcgcg gcgcgtatca tgcgccgttc
     7081 cgcggggtcg tagttcagtt ggttagaacg ccggcctgtc acgccggagg tcgcgggttc
     7141 gagtcccgtc ggccccgcca gtcttcgcta tcgatccgcg agacccggca gggactttgc
     7201 ccgtgcgggt tccgggcgcg gcctcgcacg ctcctcccag atgcggtccc agccgagcct
     7261 tcgcgccgtt tcgagattga cgcttccgtc cgcgttgtac gtgccgttcg agacgagcat
     7321 ctgcctctga ccgtccgtga gcgacggcgg cattctcgcg tcggcgctcg ccggcgcgta
     7381 ctcgttgccg gcaacgatcg gagcgaggtc cttgtgcgtc tttcggacgc cattaacgag
     7441 gtagaagaac tcgacgttcc gggagctgcg aaggacgatc ctatcgagcc cgacccgcgc
     7501 gacggcgatc accgccatgt caccgatcgg agtcgcgacg acggagagac tgtcgggatc
     7561 ggtcacgagc cggaagtctt cgggcaccgc gatcgtggcg acgccgttct ggaatctgcc
     7621 ttttccccgg aagtaggtcc cggcctcggg cccctcgagc gcgatgtagc ggatgacctt
     7681 cgaggcgtcc gtgggatgcg gctcgacgaa gtactttgcc cccgttccgc cgtaatcgcc
     7741 ggcggcgaag acggcatagt tgttcattcc cgcgtcgtaa ccgaggtgcc ccgaggcgat
     7801 cggggtgccg ttgccgaagt tgagaagctg tccggtcact gcacgctgtc cggcgtcatg
     7861 gtcttcgctc aagccgagga cgccggtcga gaatcggctg tctccccgaa cgccgatcgt
     7921 gccgcccggc ccgctcggag acacggtcat accgttgacg tcgatcccga ggacccccgc
     7981 cgccccgttg acgacgctgt tgctctcgcc cgtgattccg tagttccgtc cgggagcgag
     8041 gccgccggcg tatccccaca ctccggctga gtcgttcgac gacgagttcg tcgcgccgac
     8101 gacgatgccc gtcggtgccc actgaactcc gtagacacct gtcgaggcgt cgaagtcgtt
     8161 ctcagagatt ccccagacgc caatgaccgg cccgcaaccc ggagcgcccg aagaaagtgt
     8221 ctcaccgacg actcccgccg agcccgtgtg acaggcttct gaaaagaacg ctcctccgtt
     8281 cgagtaactg ttcgtcgtgt tcgtattcca cgcgaggacc acgcccgaac cgggcgacgg
     8341 accgcccggc acgttgccga tcgcacggaa cggtaccccg tcgttgtagc tgtccgtgaa
     8401 gtagccgttt atgtcgatgt agacgtcggt gttcgtgttc gccgtgaggc tgatcgaacc
     8461 gcccgtgccg gtgggtacca cctcggcgtt ggcgatgact tcgccggcga gatagttcac
     8521 ggccgagacc gtcggcatcg cggagcccgt tggccacgcc gagagaaatc cgtctgctgc
     8581 cggcaggatt gccccgaggt tcagcgagta ggcgtcgacg ccggcgggga tgccgggaca
     8641 gtgcggagcc gagttgaggt cgaagtctcg ggtggcatgg gcgacgagcg ccggaggccc
     8701 gtacgcgccg gtgaaaggtt cgccgccccg cgtgtcgacc aggcggcagg gtgtgacggc
     8761 gacgaatccc acccccggcg agatgtcgct catcggagag acgccgcccg ccacggcgcc
     8821 gcgataggcg ggaacggtcc agttcgtcac cgggatgcgg ggagattgtg cgagcagcgt
     8881 cgtcgagtag gcacacgcga ggcaggagag aacggttctc ttgcgaaggt acgtcatgac
     8941 tcccctccga gattggtgct cgaatcctac gccccgcggc aggtcgagag gtggggaggg
     9001 gagagcgaga tcggcagtga tgctcagtcg cagtgcccgg ggccgagggc tccttcgttg
     9061 taaccggtca gcgcctgcgt gaggtcccac gtcgccgcga gctccaggta accggagccg
     9121 cccggcggta ccacgaggtc tccgatgacc ttgtccgctt ccgcgacgaa gctcgcgacg
     9181 acgatggagt cggttccgtt tgcgatgttc agctttgccg cgatcagctg gtgcgcgagc
     9241 gcgatcaggc cgtttccgtc ggcgggagtc tcgaggatcg cctgcaggtc aacgtcggaa
     9301 tacgtcacat tgccgagcaa caccgaggag acactccatt catccgcgtt gccccggcga
     9361 gggagaggac cgtggtcctt ccagtaggcc gccgtgacga cgcagcccgc gaccgccgga
     9421 gtacacgcca aagtcccgca gaggagatcg ggcgagacgg agagagcgat cggcgccgct
     9481 gcgccccgcg caagcgcacg aaacacgtaa gacgatccac ccgcgagcga tcccgcgcag
     9541 gtcgtcggcg caccgctgtc gccgaggagc tgaccgactt cgacggtggt gcattcgccg
     9601 ccgccgagct cgggcttctt gaatcgcgcc cggccgacga aggatgccgt gcagaggtcc
     9661 gcgtcatcgg acgccggcca cgttgagtcg tcggcggttc cgggtagacc gtcgggaccc
     9721 gctgcgagcg attcggccgt cgtccactgg atcgcgaaac cgcccggcgc gcctccgggt
     9781 ccggcgcaca cctggatggc gatcgacaga ccggtcgcgc cggcgcacgt gagggaaggc
     9841 gccggc
//
LOCUS       DASWAV010000667         6468 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U2665
            k141_239111, whole genome shotgun sequence.
ACCESSION   DASWAV010000667 DASWAV010000000
VERSION     DASWAV010000667.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960316
            Sequence Read Archive: SRR10260007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 6468)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6468)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:47:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,929
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6468
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_239111"
                     /isolate="SMAG_U2665"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.36444 N 132.31889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1177)
                     /locus_tag="VF958_14080"
     CDS             complement(<1..1177)
                     /locus_tag="VF958_14080"
                     /inference="COORDINATES: protein motif:HMM:NF012783.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nodulation protein NfeD"
                     /protein_id="HEX9150280.1"
                     /translation="MTTCSAMLASLALLAGVASTPSRTPAASPIPAGRVSKVIVADVD
                     TPIHPASANYLKKVLRAAGEEGAALVIVELSTPGGLLTSTREMTSAILRSKVPVVTYV
                     APSGAQSASAGFFILLSGDVAAMSPGTNAGAAHPVGGEGQDLPKTINEKAEQDARAFI
                     RSLARQRGRNAEKAEAAVAKSVSYTETEAKEAGLIELIARDVPDLVKQLEGRSIRRLD
                     GSTAVLKLAGARVETREMSRTEKLIGIVSHPNVAYLLFLAGLLGIYFELSSPGAVLPG
                     VVGGIALLLALYAFSVLPVNLAGLALIIFAIILFIAEVKVVSHGVLAAGGAVALIAGS
                     LLLFSGRGEETIYRIDLSIIVPALVLTLAVVGFLTWKTLEIRMRPARTGGEGMVGQTA
                     "
     gene            1211..2092
                     /locus_tag="VF958_14085"
     CDS             1211..2092
                     /locus_tag="VF958_14085"
                     /inference="COORDINATES: protein motif:HMM:NF025736.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HEX9150281.1"
                     /translation="MRLRPLESVNLAALAVLTVVTLLLWPRLADPGEFLLRFSLMGAF
                     LAVVVFLTQLPRPLPKALQVALDFYPAAFIPFVYESLGPLIAAARGRARDDLLIAADR
                     TILGDDVTVLLQPFVRPGLAASLYVAYSTYYFLPIVLGAALWRKSRPEARRFIFTLTV
                     CFYVSYAGYFTIPAYGPRTALADRHTVPLNTNPVARAIDRTINQLEQTKLDAFPSGHT
                     MITVAVLIVACKRARRLFWFLLPVALMLIFSTMYCRYHYVVDVIAGIALAFATVPLGD
                     RLYGSHSRRQMAWSDVR"
     gene            complement(2093..3061)
                     /locus_tag="VF958_14090"
     CDS             complement(2093..3061)
                     /locus_tag="VF958_14090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HEX9150282.1"
                     /translation="MQLFVARRGETDVGRIAAILDRAHNDFHGEKTAFFGFFECENDP
                     EIAGLLFDAAALWAKERRMTTLRGPANPSLNDEAGLLVDGFDSPPVFMMTYNPRYYVE
                     LVEGAGFRKAKDLLAYWFEVGKGPLERLTRLADRVRQREPDVRVRSISKKSLVADLPK
                     IREVYNAAWEKNWGFVPMTPEEMEFMASRMKPLLDEEFAFLGERRREDGSLEPIAFLL
                     AMPDYNVAVAATKGRLLPFGWLKFLLAQKKIKTLRVLTLGIKREYRMRGINAVMFESG
                     LRASLKRGLTGVEISWLLEDNDLVIRSVRLWGGHLYKTYRMYERAI"
     gene            complement(3229..4467)
                     /locus_tag="VF958_14095"
     CDS             complement(3229..4467)
                     /locus_tag="VF958_14095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015721049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase
                     family protein"
                     /protein_id="HEX9150283.1"
                     /translation="MSKIAPAAKEEVRSTPLFEKCHSYDVADRVRRAGVYSFFRVIES
                     AQDPEVVSDGRRMIMLGSNNYLGLTNDPRVKEAAIEAVRKYGSGCAGSRFLNGTLDLH
                     VRLEERLAKFMRRDAAITFATGFQVNLGAISCLVAKGDVVYLDKQDHACILDGARLSF
                     GAVRKFKHNDPADLSRQMRNDVGAHGRLIVVDGVFSMEGDIAPLPEIIAAAREFEAAL
                     MVDDAHGIGVLGKNGRGTAEHFGVEDEVDLVMGTFSKSMASVGGFIAGDATVIDYIKH
                     RARTMIFSAAPPPASVAAALATVEIMEREPERRARLWENARFFSDGLRSLGLDTAGSQ
                     TPVVPIVVGEDHVALGMVQRLHEEGVFVNCVLSPATPPGRALIRTSLMATHTREQLTR
                     ALEAIEKVGRELSVIRAVGV"
     gene            complement(4594..5577)
                     /locus_tag="VF958_14100"
     CDS             complement(4594..5577)
                     /locus_tag="VF958_14100"
                     /inference="COORDINATES: protein motif:HMM:NF016228.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX9150284.1"
                     /translation="MPAAFVTGGTGFVGWHVAKTLLAEGWTVRALARGAPSRRSGLED
                     LSVEIVAGDLASAGDFGRALAGCDAVIHAAGLLKARALADYRAVNVSGTERILAAASA
                     ACPDATFVYVSSQAAAGPARDGRPVAEGDAAKPVSWYGISKLEGEQAIARDWKGRWVV
                     VRPGVLYGPRDRGLLTYFRMAARGFVPVPAASTRIQAGAIEETALGIVRAASRRDLAG
                     RIGFLCDPRPLTVREFASAIAAAANRPVRVIDIPDAAVRMAGAVESLRETLTGASRPF
                     NADKAREILAGDWLCDPAPLRRDLGLPNPPPLEDGLRAAWNWYRANAWIAS"
     gene            complement(5584..>6468)
                     /locus_tag="VF958_14105"
     CDS             complement(5584..>6468)
                     /locus_tag="VF958_14105"
                     /inference="COORDINATES: protein motif:HMM:NF012382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEX9150285.1"
                     /translation="ACQVLFEAGDEVALFCPYWVSFPEIVRLSGASPLLVPTELETRW
                     KPVASDLVERASDRTRGVIVNSPNNPTGAVVEPEELESLVEWCDARDAALIFDETYDR
                     FLYDGRQHSSAISLWEEYPHRIVVTGAASKTWAMTGWRLGWVIAGRELVEAMTSYQSH
                     STSNASSISQRAALAALSDPARTEEAVAAMLAEYGRRRDAMISGLCAVEGIRVLPPEG
                     AFYAFADVSELYAAKGVAGSVEFCDRLLAETGVAAVAGEAFGEDDCVRLSFATSLTAI
                     EEGIRRITAWAPVSVHSA"
BASE COUNT         1040 a         2242 c         2156 g         1030 t
ORIGIN      
        1 gcgcggtctg cccgaccatc ccctccccgc cggtccgcgc gggacgcatc cggatctcga
       61 gcgtcttcca cgtgaggaac ccgacgaccg cgagcgtcag cacgagcgcg ggcacgatga
      121 tcgagaggtc gatccggtag atcgtctcct cgccgcgccc ggagaacagg agaagggatc
      181 ctgcgatcaa tgcgaccgcg cctccggcag cgagcacgcc gtggctcacg accttcacct
      241 ccgcgatgaa gaggatgatc gcgaagatga tcagcgcgag cccggcgaga ttcaccggga
      301 gcaccgagaa cgcgtaaagc gcaaggagaa gcgcgatccc gccgacgacg cccggcagca
      361 ccgcgccggg gctcgacagc tcgaagtaga tgccgaggag gccggcgaga aagagaaggt
      421 acgcgacgtt cgggtgcgag acgatcccga tcagcttctc ggtccggctc atctcgcgcg
      481 tctcgacccg ggccccggcg agcttcagga cggcggtcga gccgtcgagc cgcctgatcg
      541 atcgtccctc gagctgcttg acgaggtcgg gcacgtcacg cgcgatcagc tcgatcaggc
      601 cagcctcctt cgcctcggtc tccgtgtagg acacgctttt cgcgaccgct gcctccgcct
      661 tctcggcgtt cctcccccgc tgccgggcga ggctgcggat gaacgcgcgg gcatcctgct
      721 ccgccttctc gttgatcgtc ttgggaaggt cctgcccctc cccgccgacc gggtgcgcgg
      781 cgcccgcgtt cgtgcccggg gacatcgccg cgacgtctcc cgagaggagg atgaagaatc
      841 ccgccgaagc ggactgcgcg cccgaaggcg cgacgtaggt cacgaccggg accttcgacc
      901 ggagaatcgc cgacgtcatc tcgcgcgtgg acgtgagcag tcctccgggg gtcgaaagtt
      961 cgacgatcac gagggccgca ccttcctccc ccgctgcgcg caggaccttc ttcaggtagt
     1021 tggcggacgc gggatggatg ggcgtgtcga cgtccgcgac gatcaccttc gatacacgac
     1081 cggccgggat ggggctggcc gcgggcgtcc gggatggcgt gctcgcgacc cccgccagta
     1141 gtgcgagcga cgcgagcatc gccgagcaag ttgtcatcgg ccggaaaatt ctatcgtgta
     1201 acctgccctc gtgcggctgc ggcccctgga atcagtgaac ctcgcggcgc tcgccgtcct
     1261 caccgtggtc acgctcctgc tgtggccccg actcgccgat cccggagagt ttctcctccg
     1321 gttctccttg atgggcgcgt tcctggccgt cgtcgtcttc ctgacgcagc ttccacggcc
     1381 gctcccgaag gcgctgcagg tcgccctcga cttctatccg gcggcgttca tcccgttcgt
     1441 ctacgagagc ctgggaccac tgatcgccgc cgcccgcggt cgggcgcggg acgatctcct
     1501 gatcgcggca gaccggacga tcctcgggga cgatgtcact gtccttctcc agcctttcgt
     1561 gcgacccggg ctcgcggcgt ccctctacgt cgcgtactcg acgtattact ttctgccgat
     1621 cgttctcggg gccgccctct ggcggaagag ccgtccggaa gcgcggcggt tcatcttcac
     1681 cctgaccgtg tgcttctacg tctcctacgc cgggtatttc acgatcccgg cgtacggccc
     1741 gcggaccgcg ctcgccgacc ggcacaccgt ccctctgaac acgaatcccg tcgcgcgcgc
     1801 gatcgatcgg acgatcaacc agctcgagca gacgaagctc gacgcgttcc cgtccgggca
     1861 cacgatgatc acggtggcgg ttttgatcgt ggcatgtaag cgcgcgcgga ggctcttctg
     1921 gttcctgctc cccgtggcgc tgatgctgat cttctcgacg atgtactgcc ggtaccacta
     1981 cgtcgtggac gtgatcgccg ggatcgcgct ggcgtttgcg accgtgccgc tgggggatcg
     2041 gctgtacgga tctcactcgc gccgtcagat ggcgtggtca gacgttcgct agttagatcg
     2101 cccgctcgta catccgatac gtcttgtaca ggtgcccgcc ccagagcctc acggagcgga
     2161 tgacgaggtc gttgtcctcg aggagccacg agatctcgac gccggtcagg ccgcgcttca
     2221 acgacgcgcg caggcccgac tcgaacatga ccgcattgat cccgcgcatc cggtactcgc
     2281 gcttgatccc gagcgtcagg acccgaagcg tcttgatctt cttctgcgcg agcaggaact
     2341 tgagccaccc gaacggcagc agcctcccct tcgtcgccgc cacggcgacg ttgtaatcgg
     2401 gcatcgccag caggaacgcg atgggctcga gcgagccgtc ttcgcgccgg cgctcgccga
     2461 gaaacgcgaa ctcctcgtcg aggagcggct tcatgcggct cgccatgaac tccatctcct
     2521 cgggcgtcat cggcacgaag ccccagtttt tctcccaggc ggcgttgtag acctcgcgga
     2581 tcttcggcag gtcggcgacg agactcttct tactgatcga ccggacgcgg acgtccggct
     2641 cgcgctgccg gacccggtcg gcgaggcgcg tcaggcgctc gagcggcccc ttcccgacct
     2701 cgaaccagta cgcgagcaga tccttcgcct tgcggaagcc ggcgccctcc acgagctcga
     2761 cgtagtaacg cgggttgtac gtcatcatga agacgggggg cgagtcgaag ccgtccacga
     2821 gaagtcccgc ctcgtcgttc agcgacgggt tcgccggccc ccgcagcgtc gtcatccggc
     2881 gctccttcgc ccagagggcg gccgcgtcga agaggaggcc tgcgatttcc ggatcgttct
     2941 cacactcgaa gaagccgaag aaggcggtct tttccccgtg gaagtcgttg tgcgcgcggt
     3001 cgaggatcgc cgcgatgcgg ccgacgtccg tctcgccccg ccgggcgacg aagagctgca
     3061 tctcggcgtg gcgaaagaac gggttctcgt cgagaaagac ctttgcgagg tcggagcgca
     3121 gcggcgcgac ccagttcggg tcgttgcgat agagccgctc cgcgacgtcg aggaaacgat
     3181 cgaggtcgcg cttgccgcga ccgatccgct ggaccgagac cggcgccgtc agacgccgac
     3241 ggcccgaatc acggagagct cgcggccgac tttctcgatc gcttccaggg cgcgcgtgag
     3301 ctgctcccgc gtgtgagtcg ccatgagcga cgtccggatg agcgcgcgac cgggcggagt
     3361 cgcgggcgag aggacgcagt tcacgaacac gccctcctcg tgcagtctct ggaccatccc
     3421 gagcgccacg tggtcttctc cgaccacgat cggaaccacg ggagtctgcg acccggcggt
     3481 gtcgagcccg agcgacctca ggccgtcgga gaagaaccgc gcgttctccc agaggcgcgc
     3541 ccgccgctcc ggttcgcgct ccatgatttc gaccgtcgcc agcgcggcgg ccacggaagc
     3601 cggcggcggc gcagccgaga aaatcatggt ccgtgcgcgg tgcttgatgt agtcgatgac
     3661 cgtcgcgtcg ccggcgatga accctccgac cgacgccatg gatttcgaga aggtgcccat
     3721 cacgaggtcg acctcgtcct cgacgccgaa gtgctcggcg gtcccgcggc cgttcttgcc
     3781 gaggacgccg attccgtgcg cgtcgtcgac catcagggcg gcttcgaact cgcgcgcggc
     3841 cgcgatgatc tcgggcagcg gggcgatgtc cccttccatg gagaagacgc cgtccacgac
     3901 gatcaatcgt ccgtgtgccc cgacgtcgtt gcgcatctgg cgcgagaggt cggcggggtc
     3961 gttgtgcttg aacttgcgga cggcaccgaa ggagaggcgc gcgccgtcga gaatgcacgc
     4021 gtggtcctgc ttgtcgaggt acacgacgtc gcccttggcg acgagacagg agatcgcgcc
     4081 caaattcacc tggaacccgg tcgcgaacgt gattgccgca tccctgcgca tgaacttcgc
     4141 caggcgctcc tcgagccgga cgtggaggtc gagggtgccg ttcagaaagc gggatccggc
     4201 gcagcccgat ccatatttcc ggacggcctc gatcgcggct tccttcacgc gcggatcgtt
     4261 ggtcaacccg aggtagttgt tcgacccgag catgatcatc cgacggccgt ccgagacaac
     4321 ctccggatcc tgcgccgact cgatcacccg gaagaaggag tagacgccgg cccgccgcac
     4381 gcggtctgcg acgtcatagc tatggcactt ctcgaagaga ggggtcgaac gaacctcttc
     4441 cttcgccgct ggagcgatct tgctcattgg cctcctccca cctgcgctcg agatcgaagc
     4501 cgatagtagc aaattttaca aaatgcaagt gatgcagaac acccggaacc gggtcgcggg
     4561 agcggttgct ccagctcttt tgtgcggagc gacttagctc gcgatccagg cgttcgcgcg
     4621 gtaccagttc cacgccgccc ggagcccgtc ttcgagaggg ggcgggttcg gcagcccgag
     4681 gtcgcggcgc agcggagcgg ggtcgcagag ccagtcgccg gcgaggatct cccgcgcctt
     4741 gtctgcgttg aacgggcgag atgctccagt cagcgtctcg cggagcgact ccacggctcc
     4801 ggccatccgg acggcggcgt cgggaatgtc gatgacccgg acgggccggt tcgccgcggc
     4861 ggcgatcgcc gaagcgaact cgcgcacggt cagaggccgc gggtcgcaga gaaacccgat
     4921 acgcccggcg aggtcgcgcc gggaagccgc gcgcacgatc ccgagcgctg tctcctcgat
     4981 ggcgccggcc tggatccggg tgctcgcggc gggcaccggc acgaagccgc gcgccgccat
     5041 ccggaagtac gtgagcagtc cccggtcgcg cggaccgtag agaactcccg gacgcacgac
     5101 gacccagcgg cccttccaat cccgcgcgat cgcctgctcc ccttcgagct tcgagatgcc
     5161 gtaccaggaa accggcttcg ccgcgtcccc ttccgcgacc gggcgtccgt cgcgcgcggg
     5221 gccggcggcc gcctggctcg acacgtaaac gaacgtcgcg tcgggacagg ccgccgatgc
     5281 ggcggcgaga atccgctcgg ttcccgagac gttgaccgcc cggtagtcgg caagcgctcg
     5341 cgccttcaga agccccgcgg cgtggatcac ggcgtcgcag ccggcgaggg cgcgtccgaa
     5401 atctcccgcg gacgccaggt ccccggccac gatctcgact gagaggtcct cgagcccgga
     5461 acgccgggag ggtgcgcctc gggcgagagc ccgcacggtc catccctcgg cgaggagtgt
     5521 cttcgcgacg tgccagccga cgaagcccgt gccgccggtg acgaaagccg ccggcatcga
     5581 cgctcaggcg gagtgaacgg agaccggggc ccaggcggtg atccgccgga tcccctcctc
     5641 gatcgcggtg agcgacgtcg cgaacgacaa ccgcacgcag tcgtcctctc cgaaggcttc
     5701 gcccgcgacc gccgcaacgc ccgtctcggc gagcagcctg tcgcagaact cgaccgagcc
     5761 cgcgaccccc ttggcagcgt agagctccga cacgtccgca aacgcgtaga aggcgccctc
     5821 gggcggaaga acccggatcc cctcgaccgc gcagagaccc gagatcatcg cgtcgcgtcg
     5881 gcgcccgtac tcggcgagca tcgccgccac cgcttcctcg gtccgagccg ggtccgaaag
     5941 agccgcgagc gccgcccgct gcgagatcga cgaagcgttc gaggtcgaat ggctctggta
     6001 cgaggtcatg gcctcgacga gttcgcgccc ggcgatgacc cacccgagcc gccagccggt
     6061 catggcccag gtcttcgatg cggcgcccgt caccacgatc cgatgggggt attcctccca
     6121 gagcgagatg gccgaggagt gctgccgccc gtcgtagagg aagcggtcgt acgtttcgtc
     6181 gaagatgagc gccgcgtcac gggcgtcgca ccattcgacg aggctctcga gttcttccgg
     6241 ctcgacgacg gcgcccgtcg gattattggg agagttgacg atgacgccgc gggtgcggtc
     6301 gctcgcgcgc tcgacgaggt cggacgcgac cggcttccag cgggtctcga gctcggtcgg
     6361 gacgaggagc ggcgaggcgc cggagagccg gacgatctcg gggaacgaca cccagtaggg
     6421 acagaagagg gcgacctcgt cgccggcctc gaagagcacc tggcaggc
//
LOCUS       DASWAV010000668         2771 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U2665
            k141_78915, whole genome shotgun sequence.
ACCESSION   DASWAV010000668 DASWAV010000000
VERSION     DASWAV010000668.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960316
            Sequence Read Archive: SRR10260007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2771)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2771)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:47:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,929
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2771
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_78915"
                     /isolate="SMAG_U2665"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.36444 N 132.31889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>2771
                     /locus_tag="VF958_14110"
     CDS             <1..>2771
                     /locus_tag="VF958_14110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020736689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0036297 - interstrand cross-link repair
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="crosslink repair DNA glycosylase YcaQ family
                     protein"
                     /protein_id="HEX9150286.1"
                     /translation="RVLVSPAPGEPGKMPFWHGDRPGRPPEFGRAIGELARTVAEMPK
                     RDALQRLTKDHGLDRRAAANLRAYLDEQSGATGEVPSDRAIVVERYLDEVGDWRVCIL
                     SPFGARVHAPWATAVLAKLKSSGGSDTEMLWSDDGIVFRLPESEEPPDVGDFFPDSDE
                     VEDLVTESLGETSLFAARFRENAARALLLPRRHPGRRSPLWSQRKRAADLLAVASRYG
                     SFPILLETYREVLRDVFDLPGLVEILRRVSTRRIRVLTVDSRTPSPFAASLLFSYVAN
                     FIYDGDTPLAERRAQALSVDQAQLKELLGEAELRELLDVRSIDELERNLQRLEGRPVS
                     NLDALHDLLLALGDLTEAEIRLRSTPPDAAERWLPRLAAERRVIPITVAGERRYAAAE
                     DAARLRDALGIPPPPGLPEAFLEDVRDPLGDLVSRWARTHGPFRAEDLAARFGLPIAP
                     VRSALERLAEEGRVVEGEFLPGGRTREWCDAEVLKSLKRRSLAKLRKEIEPVDSAAFT
                     RFLLEWQNVARPRAGLDALLSAVEQLGGAPVPASALETEILPARITRYKPADLDLLCA
                     AGEVIWQGISAVGPHDGRIALFLPDRHRLLAQRPGRAEGELASKVRELLERRGALFFS
                     DLVAETGAFPRELVDALWDLVWSGEVTNDTLAPLRAYVRGPAARTDRRQFRGHSFRSR
                     RSGPPGSEGRWSLLPSAVAGSLVPTETERRTALTMSLLERYGVLTREAVHAEEIPGGF
                     SAVYTIAKAMEESGRARRGYFVAGLGAAQFALPGADDRLRALREPPPREESRTIVLAA
                     TDPASPYGAAVGWPEREGARPQRVAGAQVILHDGRLLAWLGRADRNLLTFLPESGPEA
                     AECAAAVAGALAGLVESGKRRALLVAKVDGEDPSRSPLAPFLREAGFLAGSRGFLKRM
                     ATSESRQSS"
BASE COUNT          350 a         1006 c          996 g          419 t
ORIGIN      
        1 accgcgtgct cgtttcgccg gcgccgggcg agccggggaa gatgccgttc tggcacggtg
       61 accgcccggg ccggccgccg gagttcggcc gcgcgattgg agagctcgcg cgtacggtcg
      121 ccgagatgcc gaagcgcgac gcgctccagc ggctgacgaa ggaccacggg ctcgatcgcc
      181 gcgccgcggc gaacctccgt gcttacctcg acgagcagag cggggcgacc ggcgaggtcc
      241 cgagcgaccg ggcgatcgtc gtcgagcgct atctcgacga ggtcggcgac tggcgggtgt
      301 gcatcctctc tccgttcggc gcccgcgtcc acgcgccgtg ggcgaccgcg gtcctggcta
      361 agctcaagag ctccgggggc tcggacacgg agatgctctg gtccgacgac gggatcgtgt
      421 tccgcctgcc ggagtccgag gagccgcccg acgtcggcga cttctttccg gattcggacg
      481 aagtcgagga tctcgtcacc gagagcctcg gcgagacgtc gctcttcgcc gcgcgatttc
      541 gcgagaacgc ggcgcgggcg cttctcctgc cgcggcgtca tcccgggcgt cgcagcccgc
      601 tgtggtcgca gaggaagcgc gcggccgacc ttctcgcggt cgcgtcgcgt tacggctcct
      661 tcccgatcct gctcgagacc taccgcgagg tcctccgcga cgtcttcgac ctgcccggtc
      721 tggtcgagat actccgtcgg gtttcgacgc gccgcatccg cgtcctgacc gtcgactcgc
      781 gcacgccttc gccgttcgcc gcgtcgctcc tcttctccta cgtcgcgaac ttcatctacg
      841 acggcgacac cccgctcgcg gagcgccgcg cccaggccct ctccgtcgac caggcgcagc
      901 tgaaggagct tctcggcgag gcggagctgc gcgagctcct cgacgtacgc tccatcgacg
      961 agctcgagcg aaacctccag cgtctcgagg ggcggccggt ttccaacctc gacgcgctcc
     1021 acgacctgct gctcgcactg ggagacctga ccgaggcgga gatccggctg cggagcactc
     1081 cgccggatgc cgcggagcgg tggcttccgc ggctcgcggc cgagcggcga gtcattccga
     1141 tcacggtggc gggagagcgg cgctacgccg cggcggagga cgcggcccgg cttcgcgacg
     1201 cgctgggaat cccgccgcca cccgggctcc cggaggcgtt cctcgaagac gttcgcgatc
     1261 cgctcggcga tctcgtctcg cgctgggcga ggacgcacgg cccgttccgc gcggaagacc
     1321 tcgccgcccg gttcggattg ccgatcgcac cggtccgctc ggcgctcgaa cggctcgccg
     1381 aggagggccg ggtcgtggaa ggggagttcc ttcccggcgg ccgcacgcgc gagtggtgcg
     1441 acgcggaagt cctgaagtcg ttgaagcggc gctcgctcgc gaagctgcgc aaagagatcg
     1501 aacccgtcga ttccgccgcc ttcacgaggt ttctcctcga gtggcagaac gtcgcgcgcc
     1561 cgagggcggg attggacgcg ctgctctctg ccgtcgagca gctcggggga gcaccggttc
     1621 ccgcgtccgc gctcgagacg gagatccttc cggcgcgcat tacgcgctac aagccggcag
     1681 acctcgacct cctctgcgca gcgggcgagg tgatctggca ggggatctcg gccgtcgggc
     1741 cgcacgacgg ccgcatcgcg ctctttcttc ccgaccggca ccggctcctc gcgcagcgtc
     1801 cggggcgcgc ggaaggcgag ctggcttcga aggtccgcga gctgctcgag cggcgcggcg
     1861 cgctcttctt ctccgacctc gtcgccgaga cgggggcctt cccccgcgag ctcgtcgacg
     1921 ccttgtggga cctcgtgtgg tccggcgaag tcaccaacga caccctcgcg ccgctgcgcg
     1981 cttacgtccg cggtcccgcc gcgcgcaccg atcggcgaca gttccgcggg cattcgttcc
     2041 gctcgcgacg ctcggggccg ccgggcagcg aaggacggtg gtcgctcctt ccgtccgccg
     2101 tcgccgggag cctcgttccg accgaaaccg agcgccgcac cgcgctgacg atgtcgcttc
     2161 tcgagcggta cggcgtactc acgcgcgagg ccgttcacgc cgaggagatc cccggaggct
     2221 tctccgcggt ctatacgatc gcgaaggcga tggaggagtc ggggcgcgcg cggcgcggct
     2281 acttcgtggc ggggctgggc gcggcgcagt tcgccctccc cggcgcggac gaccggttgc
     2341 gggccctgag ggaaccgccg ccgcgcgagg agtcgcgcac gatcgttctc gccgcgaccg
     2401 accccgcgag cccgtacggc gccgccgtcg gctggccgga gcgggagggc gcaaggccgc
     2461 agcgcgtcgc cggggctcag gtgatcctcc acgacgggcg tctcctcgcc tggctcggcc
     2521 gggcggaccg caacctgctg acctttcttc cggagtcggg gcccgaggcg gccgagtgcg
     2581 cggcggcggt cgcgggggcg ctcgcgggac tggtcgagag cggaaagcgc cgcgcgctcc
     2641 tcgtggcgaa agtcgacggt gaagacccct cgcggtcacc gctcgcgccg ttcctgcgcg
     2701 aggcgggttt cctcgccggc agccgcgggt tcttgaaaag aatggcgacc tccgagtcgc
     2761 gtcagtcctc g
//
LOCUS       DASWAV010000669         2409 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U2665
            k141_38599, whole genome shotgun sequence.
ACCESSION   DASWAV010000669 DASWAV010000000
VERSION     DASWAV010000669.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960316
            Sequence Read Archive: SRR10260007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2409)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2409)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:47:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,929
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2409
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_38599"
                     /isolate="SMAG_U2665"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.36444 N 132.31889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1284
                     /locus_tag="VF958_14115"
     CDS             <1..1284
                     /locus_tag="VF958_14115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9150287.1"
                     /translation="PGNPSFPNILPAIDYPGSGEPFTWEDWQPRAGITYALGANRSTV
                     LKASYARYAEALGTGLIDNVNPIAGVSYAYYGWNDANHKNLVDVGEVDTSDLLNNRGY
                     DPANPGAAVSVDSFASGIHAPTTDELLVGIDHELVPAFAVGVAYTYRKFNDLIFRSRT
                     GLTGADYVQYTTITGTLPDGTAYSAPVYRVREGVDVPPGYVYDNRNDYDQTYHGVDLI
                     LTKRLANRWMARGSFTYNWNKQHVGSGGCIDPTNTVPGQSADSGNPQTGYTAQTCADG
                     VVTGFRSTGSGDKSSVFLSSKWQFYLNAMYQLPLGFNVAGSFFGRQGYPINWWRRVIG
                     PDGELRDVAVSDLDNARYDNVYDLDLRIEKVVPITTSANVTISADCFNVTNDATILQR
                     VNRLNRGPGLAFGNTNDIKEIQSPRVWRFGLRLAF"
     gene            1367..>2409
                     /locus_tag="VF958_14120"
     CDS             1367..>2409
                     /locus_tag="VF958_14120"
                     /inference="COORDINATES: protein motif:HMM:NF013080.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase"
                     /protein_id="HEX9150288.1"
                     /translation="MKRIGRATTLAAAVLAAAFCRTRPKTESAVPAASSSSRPDVLLV
                     TIDTLRADAVGFAGNRRVETPVLDRLAARGVVFANAHAHNVVTLPSHTNILTGLLPYQ
                     HGIRDNTGFRLDPSIPTLATLLKASGYATAAFVGAFPLDSRFGLARGFDVYDDRYSRG
                     RTQLDFEMPERPASEVVPLAVAWWNEQRASPRFLWVHLYDPHAPYKPPAPFLERYRSN
                     EYLGEVAATDAALAPLLDPISSGTASPTLVVVSGDHGEALGDHGEETHGLFAYEATLH
                     VPLVVCFPGRVAPRSSSEFVRHVDIAPTVLEAAGVARPAVLIGSSLLAASNVGRETYF
                     ESFSTALNRGWAPL"
BASE COUNT          410 a          833 c          743 g          423 t
ORIGIN      
        1 cccggaaatc cgtcgttccc gaacattctg ccggcgatcg actacccggg gtcgggcgag
       61 cccttcacct gggaagactg gcagccgagg gccgggatca cctacgcgct cggcgccaac
      121 cgaagtacgg tcttgaaggc gagctacgcc cgatacgcgg aggctctcgg gaccggtttg
      181 atcgacaacg tcaaccccat cgcgggcgtt tcttacgcct actacggctg gaacgacgcg
      241 aaccacaaga acctggtcga cgtcggcgag gtcgacacga gtgatctgct caacaaccgc
      301 ggctatgacc cggccaatcc gggagcggcg gtgtcggtcg acagcttcgc ctcgggtatc
      361 catgcgccga cgaccgacga gttgctggtc ggaatcgacc acgagctcgt tcccgccttt
      421 gcagtcggag tggcctacac gtatcgcaag ttcaacgatc tgatcttccg ctcgcggacg
      481 gggctcaccg gagccgacta cgtccagtac accaccatca cggggacgct tcccgacggc
      541 actgcctata gcgcacccgt ctaccgggtc cgcgagggcg tcgacgtgcc gccgggctac
      601 gtgtacgaca acaggaacga ctacgaccag acgtaccacg gcgtggatct catcctgaca
      661 aagaggctcg ccaatcgctg gatggcgcgc ggcagcttca cctacaactg gaacaagcag
      721 cacgtgggct cgggcggctg tatcgatccc acgaacacag tgcccggtca atcggcggat
      781 tccggtaatc ctcagacggg ctacacggcg cagacctgcg ccgacggcgt cgtcaccgga
      841 ttccgctcca cgggcagcgg cgacaagtcg agcgtgttcc tttcttcgaa gtggcagttc
      901 tacctgaacg ccatgtatca gctcccgctc ggcttcaacg tcgccggcag cttcttcggc
      961 cgccagggat acccgatcaa ctggtggcgt cgggtcatcg gtccggatgg ggaactgcgt
     1021 gacgtggccg tgtcggacct cgacaatgcg cggtacgaca acgtctacga tctcgacctg
     1081 cgcatcgaga aagtcgttcc gatcaccact tcggccaacg tcaccatctc tgcggactgc
     1141 ttcaacgtga cgaacgatgc cacgatcctc cagcgggtca accggctcaa ccggggtccc
     1201 ggactggcgt tcggcaacac gaatgacatc aaggagattc agagcccgcg ggtgtggcgg
     1261 ttcgggctgc ggctcgcgtt ctaagtcttc tcttcgcctc ttcggccgcc gggaatacgc
     1321 ttcccggcgg cctttttcat ttgggcgagg aaagagcagt cgaacgatga aacgaatcgg
     1381 gagagcgacg acgctggcgg ctgcggttct cgcggccgcc ttctgccgga cgcgaccgaa
     1441 gaccgaaagc gctgtaccgg ccgcatcctc ctcttcccgc ccggacgtcc ttctcgtgac
     1501 gatcgacacg ctccgggcgg acgccgtcgg attcgccggc aatcgtcgcg tcgagacacc
     1561 cgttctcgac cggctggcgg cccgcggagt cgtcttcgcg aacgcgcacg cccacaacgt
     1621 cgtgacgctt ccgtcgcaca cgaacatcct gacgggcctt ctcccgtacc agcacgggat
     1681 ccgcgacaac accggattcc gtctcgaccc ctcgattccc acgctcgcca cgctcctgaa
     1741 ggcgagcggt tacgcgacgg cagcgttcgt cggcgcgttt ccgctcgact cccggttcgg
     1801 actcgcgcgc ggattcgacg tttacgacga ccgctattcc cgcgggagga cgcagctcga
     1861 tttcgagatg cccgagcggc cggcgtcgga agtcgtgccg ctcgcggtcg cctggtggaa
     1921 cgaacagcgg gcgtcaccga ggttcctctg ggttcatctc tacgacccgc acgcgccgta
     1981 caagccgccg gcccctttcc tggagcgtta ccgctcgaac gaatatctgg gcgaggtcgc
     2041 ggcgaccgac gcggcgctcg cgccgctgct cgacccgatc tcctccggga cggcgtctcc
     2101 gaccctcgtc gtcgtctcgg gtgaccacgg cgaggcgctc ggcgaccacg gcgaagagac
     2161 ccacggcctg ttcgcgtacg aggcaacgct tcacgtgccg ctcgtcgtgt gctttccggg
     2221 tcgggtcgcc ccccgctcga gctccgagtt cgtccgtcac gtggacatcg ccccgaccgt
     2281 gctcgaggcg gcaggagtgg cgagacccgc tgtgctcatc ggatcgtccc tgctcgccgc
     2341 ttcgaacgtc gggcgcgaga cgtacttcga gtcgttctcc acggctttga accgcggctg
     2401 ggcgccgct
//
LOCUS       DASWAV010000670         3194 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U2665
            k141_79286, whole genome shotgun sequence.
ACCESSION   DASWAV010000670 DASWAV010000000
VERSION     DASWAV010000670.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960316
            Sequence Read Archive: SRR10260007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 3194)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3194)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:47:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,929
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3194
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_79286"
                     /isolate="SMAG_U2665"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.36444 N 132.31889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            68..1348
                     /locus_tag="VF958_14125"
     CDS             68..1348
                     /locus_tag="VF958_14125"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX9150289.1"
                     /translation="MKGRGLVSTIIPVFNRPAMVTEAVAGVLNQTYSDVEVIVVDDGS
                     TDETWKTCQRLALRDKRVKIRRIEHVGRPGLVREVGRVLARGEYIQYLDSDDLIPPER
                     FARMIETLNEREPADLAYCLTRRYSLGQQPEWRPAGRTGETHTRIFPEILSERPWFSS
                     SVLYRRAICDRAGPWSDLPFMEDVELDARIGRFGATVRHCPEFLADMRDHSGERVSRQ
                     DLLERTDLLKDAVRAYSMIFSHARAAGVTATEIPMILFLEEVRLLASRAGKLLLPEES
                     STCAQIIETAFGPTANAGLTLDAEIEPVRTDLTLKAGATTTCPVRIRNRSAISFREGR
                     WPVHLSYHLLSPSGDLLQFDNRRTPFQQILWPGEKRIVDLQIEAPNFPGDYVLRIDLV
                     LERVSWFEWEGVRPGIVHLRVEDATGVEPAAVDG"
     gene            1341..2741
                     /locus_tag="VF958_14130"
     CDS             1341..2741
                     /locus_tag="VF958_14130"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HEX9150290.1"
                     /translation="MVEGRQKLGRAKEIAVLALATLLGIALRLVFVFRFPAITPDGRL
                     YLAIAETWSRFGVYGTDPTTPSALRLPGYPAFLVGIHFVFGSIRPNSVFLVQTVVDLA
                     TCVLVGLIGAALFGKRVGQAAFVLALLCPFTANYVSLLLTESLAIFTTTLALLLGFVA
                     LARIGERLPVGYLLATGVAIGAGILIRPDGGILLPVLASAFLIRVRRAGLKRVIGAAA
                     IVSAVSLGFLVPWTIRNAVVLGEFQPLTPTVIVGKGERVPVGFNRWVKTWILDYVSDQ
                     DVWWKAFSHEPIDPGTLPRRAFGSEGDPARTLALIAEFNESGTVTEALDRKFDELAAE
                     RIRTHPVSYYILIPLGRMADMWLRPRTEMLPLESRWWEFRNVRNSWTSIGYAGINLIF
                     LALASYGVLSHWRRPEVRILIGFAVLRTVLLATVANPCSPEDRYTLECYPAVLALAAA
                     GLLQFRSSRAPGGVNS"
     gene            2746..>3194
                     /locus_tag="VF958_14135"
     CDS             2746..>3194
                     /locus_tag="VF958_14135"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEX9150291.1"
                     /translation="MDSRGEARRGPPPQLSIVVPVFDEEENLRPLDTEIRGVLESIGL
                     SAEIIYVDDCSRDRSLAVLKEISQGPNAPIRTRVVKLRRNFGQTAAMAAGFELAEGDV
                     IVPLDADGQNNPADIPRLVETLGRGFDVVSGWRRKRRDGTLNRIIPSR"
BASE COUNT          521 a         1045 c          997 g          631 t
ORIGIN      
        1 ccgttcggga gcgccacagc ctttcgtacg tcggcgcgcg ctatcgcgac ctcttcgacg
       61 agatccgatg aagggccggg ggctcgtctc gaccatcatt cccgtgttca accgccctgc
      121 catggtgacc gaggctgtcg cgggcgtttt gaatcaaacc tactcggacg tcgaagtaat
      181 cgtcgtcgac gatggttcga ccgacgaaac ctggaaaacc tgccagagac tcgcgctccg
      241 ggacaaacga gtcaagatcc gccggatcga gcacgtcggc agaccgggcc tggtgcggga
      301 ggtcgggcgc gtcctcgccc gcggtgagta catccagtat ctcgacagcg acgatctgat
      361 accgcccgag cggttcgcca ggatgatcga aacgctgaac gagcgggagc cggccgacct
      421 cgcctactgc ctcacgcgcc gctattcgct cggccagcaa cccgaatggc gtcccgccgg
      481 ccgcacgggc gaaactcata cccggatatt cccggagatc ctttcggaaa ggccctggtt
      541 cagctcgagc gttctgtacc gccgcgcgat ctgtgaccgc gcgggaccct ggtcggacct
      601 gcccttcatg gaagacgtcg agttggacgc acgaatcggg cggttcggtg cgaccgtccg
      661 acactgcccc gaattcctgg cggacatgcg agaccattcg ggagagcgcg tctcacggca
      721 ggatctcctc gagcgaaccg acctcttgaa ggacgcggtc cgggcgtatt cgatgatctt
      781 ctctcacgcg cgagccgctg gcgtaacggc gaccgaaatt cccatgattc tctttctcga
      841 ggaagtccgc ctgctcgcga gccgcgccgg caagctgctc cttccggaag agtcttcgac
      901 ctgtgcgcaa atcatcgaga ctgctttcgg cccgaccgcg aacgcaggac tgaccctcga
      961 cgcggagatc gagccggtcc ggacggatct caccctgaag gcgggcgcga cgacgacgtg
     1021 tcccgtccga atccggaacc gatccgccat ctccttccga gaaggtcgct ggcccgtgca
     1081 cctttcgtat cacctcctct caccgtccgg agaccttctc caattcgaca accggcgaac
     1141 acccttccaa cagatcctct ggccgggcga gaaaaggatc gtcgaccttc agatcgaggc
     1201 gccgaatttc ccgggcgact acgtcctccg gatcgacctc gttttggagc gcgtcagctg
     1261 gttcgaatgg gagggggtcc gaccgggcat cgttcacctc cgtgttgagg atgcgacggg
     1321 agtggagccg gcagcggtcg atggttgagg gccgtcagaa gctgggtcgg gcgaaggaga
     1381 tcgccgttct cgcgctcgcc actctcctgg ggatcgcgct gcggctggtc ttcgtctttc
     1441 ggtttcctgc catcaccccg gacggccgtc tttatctcgc aatcgcggag acgtggtctc
     1501 gattcggggt ctacgggacc gatccgacga caccgtccgc gctccggctg cccggttacc
     1561 cggcctttct cgtcggaatt cactttgtgt tcggatccat ccggcccaat tcggtcttcc
     1621 tggttcaaac ggtggtcgac ctggcgacct gcgtgctggt tggtttgatc ggcgcggctc
     1681 tcttcggcaa gcgggtcggt caggccgcgt tcgttctcgc tctgttgtgc cccttcacgg
     1741 cgaactacgt ttcactcctc ctgaccgaat cgctcgcgat cttcacgacg accctcgcgt
     1801 tgctgctcgg gttcgtcgcg ctcgcgcgaa tcggcgaacg tttgccggtc ggttacctcc
     1861 tcgccaccgg ggtggcaatt ggagccggaa tcctcatccg tccggatggc gggattctgc
     1921 ttccggtact cgcgtccgcc tttctgatcc gggtgcgccg cgctggcctg aagagggtca
     1981 tcggcgcggc ggcgatcgtc tctgcggttt cgctggggtt cctcgtgccg tggacgatcc
     2041 ggaacgccgt cgtgctcggc gagtttcaac ctctgacgcc gaccgtgatc gtcggaaagg
     2101 gcgaacgtgt ccctgtgggc ttcaatcggt gggtgaagac gtggattctc gactacgttt
     2161 ctgaccagga cgtctggtgg aaggcatttt cccatgaacc gatcgatccc gggaccctgc
     2221 ctcgccgcgc cttcgggtcc gaaggtgacc cggcccgaac gctcgcgctg atcgcggagt
     2281 tcaacgagag cggaacggtg accgaagccc tcgaccggaa gtttgacgag cttgccgcag
     2341 agaggatccg cacgcatccc gtgagctatt acatcctgat tccactgggc cgaatggccg
     2401 acatgtggct ccggccgagg acggagatgc tccctctcga gagccgatgg tgggagttcc
     2461 gtaacgtgag gaactcctgg acttcaatcg gttacgcggg catcaacctg atctttctcg
     2521 ccctcgcctc ttatggcgtg ttgagccact ggcgacggcc cgaagtgcgg atcctgatcg
     2581 gttttgccgt tctgagaacg gtcctgctcg cgaccgtcgc aaatccctgc agcccggagg
     2641 accgatatac gctcgaatgc tatcctgcgg tgttggctct cgcagctgcg ggtctcttgc
     2701 agttccgatc ttccagagca cccggtggcg tcaattcctg aagtagtgga ttcccgcggc
     2761 gaggcgcgcc ggggcccgcc gccgcagctc tccatcgtcg ttcccgtgtt cgacgaggaa
     2821 gagaacctcc gcccgctcga caccgagatc cgcggcgtcc tcgagtccat cggcctttct
     2881 gccgagatca tctacgtgga cgactgttcg cgcgaccgat cgctcgcggt cctgaaggag
     2941 atctcgcaag gccccaacgc tccgatccgc actcgcgtgg tcaagctccg ccgtaacttc
     3001 ggtcagacgg ccgccatggc ggcggggttc gagctggcgg agggagacgt catcgtgccg
     3061 ctcgatgcgg acggccagaa caatccggcc gacattccgc ggctggtcga gacgctcggc
     3121 cggggcttcg acgtcgtgag cggctggagg cgaaaacgcc gggacggcac cctgaaccgg
     3181 atcatcccga gccg
//
LOCUS       DASWAV010000671         3168 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U2665
            k141_119214, whole genome shotgun sequence.
ACCESSION   DASWAV010000671 DASWAV010000000
VERSION     DASWAV010000671.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960316
            Sequence Read Archive: SRR10260007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 3168)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3168)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:47:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,929
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3168
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_119214"
                     /isolate="SMAG_U2665"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.36444 N 132.31889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..749)
                     /locus_tag="VF958_14140"
     CDS             complement(<1..749)
                     /locus_tag="VF958_14140"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEX9150292.1"
                     /translation="MTFARMTVGLDGSPHAEAALSHAIRIASASGGLVRGIHVVDRSM
                     LDGVVVADLSGSVGFQPFLNLSGELRDALVSAGGAILAAFEARAKEAGIAFEPVLREG
                     NVSEILNEEARMSDLAAIGSRGANAAHRRDLVGRHADALARRMPTPLLIAPAEYRDFR
                     TPLLAWDGSPKARKALVLAAEVAALFALPVRVVTATDDEDEGNALLAEAEEELRYRRV
                     TVSSIRRAAHPDDAIFAEIGAGADLVAMGAHG"
     gene            complement(746..1552)
                     /gene="amrB"
                     /locus_tag="VF958_14145"
     CDS             complement(746..1552)
                     /gene="amrB"
                     /locus_tag="VF958_14145"
                     /inference="COORDINATES: protein motif:HMM:TIGR04336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system protein B"
                     /protein_id="HEX9150293.1"
                     /translation="MPVRQPAVAGLFYDSQPERLERHVRRLLPTEVEAAPAIGAIVPH
                     AGYVYSGGVAGAVYGRLSIPATVVILCPNHTGRGAPAALDPSESWATPLGEVPLARRL
                     ADRLLELAPSLEEDSEAHRREHSLEVQLPFLRVLRPDVELVPVCLGEASLPLCAEVGD
                     ALATLVSGEKEGPLLLASSDMNHYESRQIGRAKDDLALARIEAMDAEGLFHTVLAKSV
                     SMCGFLPATALLFAARALHARRAEVVARRDSGDETGDDSTVVGYAGVVIT"
     gene            complement(1577..2401)
                     /locus_tag="VF958_14150"
     CDS             complement(1577..2401)
                     /locus_tag="VF958_14150"
                     /inference="COORDINATES: protein motif:HMM:NF014427.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HEX9150294.1"
                     /translation="MRLPGRLLCLLLLGAAAGCARKPASVQVSPRKVLLYGMDRSERL
                     TARVLDNNGRELEGLRAVWSSSAPDVAAVDEGGLVHSKKEGKATITGQVGQVTAQVPV
                     EVVDAAAIEVVPSQATLVGPPGTTFSLTAIVKNSKKAPIAVRPSWSSSNEKVVKVGPD
                     GAVTSVGPGTASVTANVGELQGATEVTVLVHDIVRLELRPATALVRVGDSQKFQVAAY
                     GADGVRLDNAMASFRSSNPAVATIDGAGVAAGVGAGTATIRVDLAGRTAEATLIVN"
     gene            complement(2552..>3168)
                     /gene="mtgA"
                     /locus_tag="VF958_14155"
     CDS             complement(2552..>3168)
                     /gene="mtgA"
                     /locus_tag="VF958_14155"
                     /EC_number="2.4.1.129"
                     /inference="COORDINATES: protein motif:HMM:TIGR02070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009274 - peptidoglycan-based cell wall
                     [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="monofunctional biosynthetic peptidoglycan
                     transglycosylase"
                     /protein_id="HEX9150295.1"
                     /translation="VFAVTLPDVRPLAKSFPSTTAFMERRRASLAQSGLSTRLEWTPV
                     PIARISPSLQRAVVAAEDARFWEHEGVDWEAIRTAALHDWSRGRISHGGSTITQQLAK
                     NLYLSPARTPWRKLKEWAIARRLEKELSKRRILELYLNVVEFGVRTYGVEAAARRYFG
                     KPASALAPSEAATLAAMIPSPRIYDPVRHPKRVERRARRIRRWM"
BASE COUNT          511 a         1093 c         1090 g          474 t
ORIGIN      
        1 ccgtgggcac ccatcgcgac gaggtccgcg cccgcgccga tctccgcgaa gatcgcgtcg
       61 tcggggtgag ccgcgcgacg gatgctcgac accgtgacgc gccggtaccg gagctcctcc
      121 tcggcttctg cgagaagggc gttgccctcg tcctcgtcgt ccgtcgccgt caccacgcgg
      181 accggcagtg cgaaaagggc cgcgacctcc gcggccagca ccagcgcctt gcgcgccttg
      241 ggcgagccat cccatgcgag aagcggcgtc cggaagtcgc ggtactcggc cggcgcgatc
      301 aagagcggcg tgggcatgcg ccgcgccagc gcgtccgcgt gacgtccgac gaggtcccgc
      361 cggtgcgccg cgtttgcccc gcgcgagccg atcgcggcaa ggtcggacat tcgcgcctct
      421 tcgttcaaga tctcggagac gtttccctcg cggagaaccg gctcgaacgc gatgccggcc
      481 tccttcgccc gtgcctcgaa cgcggcgagg atggcgcccc ccgccgaaac gagagcatca
      541 cgcaactcgc ccgagaggtt caggaacggc tggaatccca cggacccgga gaggtccgcg
      601 acgacgacgc cgtcgagcat tgaacggtcc acgacgtgga tgccccgcac gagtcctccc
      661 gaggcgctcg cgatccggat cgcgtgagag agcgcagctt ccgcgtgcgg agacccgtcg
      721 agaccgaccg tcattcgtgc gaacgtcacg tgatcaccac ccctgcgtat ccgacgaccg
      781 tcgagtcgtc gcctgtttcg tcaccgctgt cccttcgggc gacgacctcg gcgcgacgcg
      841 cgtgaagggc gcgcgcggcg aaaagcaacg ccgtcgccgg aagaaagccg cacatcgaga
      901 ccgattttgc cagcaccgtg tggaaaagac cttccgcgtc catcgcctcg atccgggcga
      961 gcgcgaggtc gtccttcgcg cgcccgatct gccgcgattc gtagtggttc atgtccgacg
     1021 aggccaggag gaggggccct tccttctccc cggagacgag agttgcaagc gcgtcgccga
     1081 cctctgcgca gagcgggagg gacgcctcgc cgagacatac cgggacgagc tcgacgtcgg
     1141 gtcggaggac ccgaagaaag gggagctgga cttccagcga gtgctcgcgg cggtgggcct
     1201 ccgaatcttc ctcgaggctc ggggcgagct ccaggagccg gtcggcgagc cggcgcgcga
     1261 ggggaacctc gccgagcggg gtcgcccagc tctccgacgg atcgagcgcg gcgggcgcgc
     1321 cgcggcccgt gtggttcggg cacaggatca cgacggtggc cgggatgctc aaccggccgt
     1381 acaccgcgcc cgcgaccccc cccgagtaga cgtaccccgc gtgcgggacg atcgctccga
     1441 tcgccggcgc cgcctcgact tccgtcggca gaagccgccg gacgtggcgc tcgagccttt
     1501 cgggctgcga gtcatagaag agtccagcca ctgcgggctg gcggacgggc atttcgggag
     1561 cgtatcccga ttccggctaa ttgacgatca acgtcgcttc ggcggtcctg ccggcgaggt
     1621 cgacgcggat cgtggcggtc ccggccccga ctccggcggc aacccccgcg ccgtcgattg
     1681 tcgccaccgc gggattcgac gatcgaaagc tcgccatcgc gttgtcgagt cgcacgccgt
     1741 cggctccata ggcggcgacc tggaacttct gcgagtcccc gacccggacc agggcggtcg
     1801 cgggccgaag ctccaggcga acgatgtcgt gaaccaggac ggtgacttcg gtcgcgccct
     1861 gcagctcgcc gacgttcgcg gtgaccgagg ccgtgcccgg gccgacggac gtcaccgcgc
     1921 cgtcggggcc gactttcacg accttctcgt tcgaagatga ccaggaggga cgaacggcga
     1981 tcggcgcctt cttcgagttc ttgacgattg cggtcaggga aaaggtcgtt ccgggaggac
     2041 cgaccagcgt tgcctgcgaa ggcacgacct cgatcgcggc ggcgtcgacg acctcgacgg
     2101 ggacctgggc cgtaacctgg ccgacctggc cggtgatcgt cgctttcccc tctttcttcg
     2161 aatggacgag gcctccctcg tcgacggccg cgacgtccgg cgccgaggag gaccagacgg
     2221 cgcgcaagcc ctcgagctcc cggccgttgt tgtcgagcac tcgcgcggtc agccgctcgc
     2281 tccgatccat gccgtaaagg aggaccttcc tcggcgacac ctgaacggag gcgggcttcc
     2341 gcgcacagcc cgcggctgcg ccgagcagaa ggagacagag cagtcgtcct gggagacgca
     2401 tcacgttcat ttaagacaag ttgcagagag gatagcacga gaggggcgcg acctcatccc
     2461 gttccgacct gacgaccgaa ctcaccatgc cgcgcaaaca aattccgtca cctgtgcgtg
     2521 agcaggtccc gagccgggcg accgcgacgg tttacatcca gcgccggatt cggcgggctc
     2581 ggcgctccac cctcttcggg tggcggaccg gatcgtagat gcgcggagag gggatcattg
     2641 ccgcgagagt cgcggcctcg gagggtgcga gtgcgctcgc cggcttgccg aagtatcgcc
     2701 gcgccgcggc ttccactccg taagtgcgga cgccgaactc gacgacgttc aggtagagct
     2761 cgaggattcg tcgcttcgag agctccttct cgagccgccg ggcgatcgcc cactccttga
     2821 gcttccgcca gggggtccgc gcgggggaga ggtaaaggtt cttcgccagt tgctgtgtga
     2881 tggtcgagcc gccgtgcgag atccggccgc ggctccagtc gtggagcgca gctgttcgga
     2941 tggcctccca gtcgaccccc tcgtgctccc agaaccgcgc atcttccgcc gcaaccaccg
     3001 cgcgctgaag agaaggcgag atcctcgcga tcgggacggg agtccattcg aggcgcgtgg
     3061 acagtcccga ctgcgcgaga gaggcgcgcc gcctctccat gaacgccgtc gtactcggaa
     3121 agctcttcgc gagcgggcgg acgtccggaa gggtgacggc gaagaccg
//
LOCUS       DASWAV010000672         1577 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U2665
            k141_357078, whole genome shotgun sequence.
ACCESSION   DASWAV010000672 DASWAV010000000
VERSION     DASWAV010000672.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960316
            Sequence Read Archive: SRR10260007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 1577)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1577)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:47:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,929
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1577
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_357078"
                     /isolate="SMAG_U2665"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.36444 N 132.31889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..352
                     /locus_tag="VF958_14160"
     CDS             <1..352
                     /locus_tag="VF958_14160"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009408552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="GTP cyclohydrolase I"
                     /protein_id="HEX9150296.1"
                     /translation="FYSMCAHHFLPFFGSAHVGYVPGKRLAGLSKFARIVDFYARRPQ
                     IQEDLTEQIAALLDRRLAPTGLIVSVEARHLCMEMRGVSRPGVTTKTMAVRGTLQDER
                     LQRRFFERLRESRP"
     gene            349..1527
                     /locus_tag="VF958_14165"
     CDS             349..1527
                     /locus_tag="VF958_14165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011985896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEX9150297.1"
                     /translation="MMHYRYLIVGGGMTADAAVRGIRELDGSSRIGLVGLEADAPYAR
                     PPLSKGLWKGEPFESVWRKTAEVGVDLHLGRRVTALDLREKRAVDDRQDVYAFDKLLL
                     ATGGTPRRLPFGGDDVTYFRTLEDYRRLRDHAGPGKAFAVIGGGFIGSEIAAALALQG
                     CKVTMLLPEEGIGARVFPPDLARFLVDYYGEKGVVVRPGEQVTDVSRKGSQVVVETKS
                     GLRVTADGVVAGLGIVPNTDLAAAAGLVVEDGIVVDEFLRATDSDVFAAGDVARFRSR
                     ALGTKMRVEHEDNALTMGRAAGRAMAGDASPFEHLPFFYSDLFELGYEAVGELDPRGE
                     VLADWKTLFREGVIYYLSGGRVRGVLLWNTWGQVDAARALIAEPGPFRAANLAGRLPS
                     "
BASE COUNT          250 a          511 c          539 g          277 t
ORIGIN      
        1 gttctactcg atgtgcgcac atcacttttt gccgttcttc ggctcggctc acgtcgggta
       61 cgtcccgggg aagcgtctcg ccggcctttc caaattcgcg cggatcgtcg acttctacgc
      121 gcggaggccg cagatccagg aggacctgac ggagcagatc gcggctctgc tcgaccggcg
      181 cctggcaccg acggggctca tcgtttcggt cgaggcgagg catctctgca tggagatgcg
      241 aggcgtgtcg aggcccggag tgaccacgaa gacgatggcc gttcgtggaa cactccagga
      301 cgagcgcctg cagcgacggt tcttcgagcg actccgcgaa agccggccgt gatgcattac
      361 cggtacctca tcgtcggcgg cggcatgacc gcggacgccg cggtccgcgg catccgagag
      421 ctcgacggga gcagtcgcat cgggctggtc ggcctcgagg cggacgcacc ctatgcccgt
      481 ccgccgctct ccaagggatt gtggaaaggc gaaccgttcg agagtgtctg gcgaaagacc
      541 gccgaagtcg gcgtcgatct ccacctcggg cgccgcgtga cagctctgga tcttcgggag
      601 aaaagggcgg tcgacgatcg gcaagacgtc tatgcgttcg acaagcttct tctcgcgacg
      661 ggagggacgc cccgccgtct tcctttcggc ggcgacgacg tgacgtattt ccgaacgctc
      721 gaggactacc gccgtctccg cgaccacgca gggccgggga aggcgtttgc ggtgatcggg
      781 ggcggcttca tcgggtcgga aatcgccgct gccctcgccc tgcaggggtg caaagtcacg
      841 atgctccttc cggaagaggg aatcggcgcg cgggtcttcc cgccggatct cgcccggttt
      901 ctcgtcgact actacggcga gaagggcgtg gtcgtccgcc cgggcgaaca ggttacggac
      961 gtcagccgga agggatcgca ggtggtcgtc gagacgaaga gcggactgcg cgtgactgcc
     1021 gacggagtcg tggcgggcct cgggatcgtc cccaacaccg acctcgccgc cgcggccggt
     1081 ctcgtggtcg aagatggcat cgtcgtagac gaattcctcc gggcgacgga ttccgatgtc
     1141 ttcgccgccg gcgacgtcgc ccgtttccgg tcccgcgcgc tcggcacgaa gatgcgcgtc
     1201 gagcacgagg acaacgcgct caccatgggc cgcgccgcgg ggcgcgcgat ggccggcgac
     1261 gcctcgccgt tcgagcacct tccgtttttc tactcggacc tgttcgagct cggttacgaa
     1321 gccgtcggtg agctcgaccc gcgcggagaa gtgttagcgg actggaagac gctcttccgc
     1381 gaaggagtga tctattacct gtcagggggc cgggtccggg gagtcctgct ctggaacacc
     1441 tggggtcagg tcgatgccgc gcgcgccctc atcgccgaac ccggtccctt ccgcgccgcc
     1501 aacctcgccg gccggcttcc gtcgtgagac tgcaattgaa atcaggaggt cccatgaaac
     1561 gctggctccc ggttctc
//
LOCUS       DASWAV010000673         2179 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U2665
            k141_480184, whole genome shotgun sequence.
ACCESSION   DASWAV010000673 DASWAV010000000
VERSION     DASWAV010000673.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960316
            Sequence Read Archive: SRR10260007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2179)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2179)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:47:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,929
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2179
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_480184"
                     /isolate="SMAG_U2665"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.36444 N 132.31889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..234
                     /locus_tag="VF958_14170"
     CDS             <1..234
                     /locus_tag="VF958_14170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009613787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diadenylate cyclase"
                     /protein_id="HEX9150298.1"
                     /translation="VAAASCFLPLSLKEGLSKRFGTRHRAAMGVTEETDALAIVVSEE
                     RGTISLARDGQLIEDLDGKSLRDLLYREFATQA"
     gene            247..948
                     /locus_tag="VF958_14175"
     CDS             247..948
                     /locus_tag="VF958_14175"
                     /inference="COORDINATES: protein motif:HMM:NF019561.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CdaR family protein"
                     /protein_id="HEX9150299.1"
                     /translation="MPRRRFLFRNMGLKLLALAIALLAWFALSGQRRERISERSYRIP
                     LSLVNVPSQTMVASPLPGGVDVRVRGPFTALRQLQPERLEAVIDLLNARAGESVHRFA
                     PEDINVPPEVEVLAISPGEVKVVLDRIGERLLPLVPALSGEPAPGAQVVDVSVDPRIA
                     RVIGPATALERMTGVSTEPVSVANRSATFSVTTTALPNVPGVRVREGQVVHVTVRMGP
                     APTPAATPGTRPAGP"
     gene            945..>2179
                     /locus_tag="VF958_14180"
     CDS             945..>2179
                     /locus_tag="VF958_14180"
                     /inference="COORDINATES: protein motif:HMM:NF014877.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HEX9150300.1"
                     /translation="MKLFGTDGLRGTAGEFPLDPGSLSIVGREVGADVRRAGARTAVL
                     GGDTRESTPAMAAQLAAGLSDAGCDVAFAGVVPTPAVAELVLALQAGAGISVSASHNP
                     YEDNGVKVFGPDGRKWPDEKEEALEERLLRGQPPHPGPVARDEKPPPVDSSLPELYLS
                     RLLSRVPNDLRSLSVLVDTGNGAASKLGPEALARAGCRVASICDRPDGRNINAGCGAL
                     HPERMARETRETGAALGVALDGDADRAIFADETGKLLDGDDVLWIVARRWKAEGRLTV
                     GGVVGTVMSNFGLEAALAREEIVFHRAAVGDRSVARLMDETGAVLGGETSGHILFAPL
                     SPAGDGIQTALVVAGVLAVAGAPLSRLATLEKAPQELRNVRVSRREPLERTPELSSAI
                     ESASATLGPRGRVFVRYSGT"
BASE COUNT          303 a          777 c          773 g          326 t
ORIGIN      
        1 gtcgcggcgg cgtcgtgctt cctgccgctc tcgctcaagg aaggcctgtc gaagcgattc
       61 gggacgcgcc accgcgccgc catgggcgtg accgaggaaa ccgacgcgct cgcgatcgtc
      121 gtctccgagg agcgcggcac gatctctctc gcgcgggacg gacagctgat cgaggacctc
      181 gacggaaagt cgctccgcga ccttctctac cgcgagtttg cgacgcaggc atagggcgac
      241 agcgttatgc cccgacgacg cttcctcttc cgcaacatgg gcctgaaact gctcgcgctc
      301 gcgatcgcac tcctcgcgtg gttcgcgctg tcgggccagc gacgggagcg gatctcggag
      361 aggagctacc gcatcccgct ctcgctcgtg aacgtcccgt cgcagacgat ggtggcttcg
      421 ccgctgcccg gaggggtcga cgttcgcgta cgcgggccat tcaccgccct ccggcagctg
      481 caacccgaga ggctcgaggc cgtgatcgac ctcttgaacg cgcgagccgg cgagagcgtg
      541 caccgcttcg cgccggaaga catcaacgtt cctccggagg tcgaggtcct cgcgatctcg
      601 cccggcgagg tgaaggtggt gctcgaccgc atcggcgagc ggctcctgcc gctcgtcccg
      661 gctctctccg gcgagccggc gccgggcgcg caggtggtcg acgtctccgt cgacccgcgg
      721 atcgcgcggg tcatcggtcc cgccacggcg ctcgaaagga tgaccggcgt ttcgaccgag
      781 ccggtctcgg tcgcgaaccg atccgcgacg ttctccgtga cgaccaccgc gctcccgaac
      841 gtgccgggcg tgcgcgttcg ggaaggtcag gtcgtccacg tcaccgtgcg gatggggccc
      901 gcgccgacgc ctgcggcgac gcccggcacc cgtccggccg gtccgtgaag ctcttcggga
      961 ccgacggact ccgcggaacg gcgggcgagt ttccgctcga tccgggatcg ctctcgatcg
     1021 tcgggcggga ggtcggagcg gacgtgcgcc gggcaggcgc gcgaaccgcg gtcctcggcg
     1081 gcgacacgcg cgagtcgaca ccggccatgg cggcgcagct cgccgccggg ctctcggacg
     1141 ccggctgcga cgtcgccttc gcgggtgtcg tgccgactcc cgccgtggcc gagctcgtgc
     1201 ttgcgctcca agccggcgcc ggaatctccg tctccgcgtc gcacaacccg tacgaagaca
     1261 acggcgtgaa ggtcttcggg ccggacgggc gcaagtggcc ggacgagaag gaggaggcgc
     1321 tcgaagaaag gctgctgcgc ggccaacccc ctcaccccgg ccctgtcgcg agagatgaaa
     1381 agccgccgcc agtcgattcc tctctccccg agctctacct ctcgcggttg ctttcgcggg
     1441 tcccgaacga tctgcgaagc ctctctgtcc tcgtcgacac cggcaacggg gcagcgtcga
     1501 agctcggccc ggaggcgctc gcgcgggccg gctgtcgcgt cgcgtcgatc tgcgaccggc
     1561 ccgacggacg caacatcaac gccggctgcg gcgcgctcca tccggagcgg atggcgcggg
     1621 agacgcggga gacgggtgcc gcgttgggcg tcgcgctcga cggcgacgcg gatcgcgcga
     1681 tcttcgcaga cgagactggc aagctcctcg acggagacga cgtcctctgg atcgtcgccc
     1741 gccggtggaa ggccgagggg cggttgaccg tcggcggtgt cgtcgggacc gtgatgtcga
     1801 acttcggcct cgaggcggcg ctcgcccgcg aagagatcgt gttccatcgc gcggcggtcg
     1861 gcgaccggag cgtcgcgcgg ctcatggatg aaaccggcgc cgtgctcggc ggtgagacgt
     1921 ccgggcacat cctcttcgcg ccgctctccc ccgccggcga cggcatccag acggcgctcg
     1981 tcgtcgcggg agttcttgcc gtcgccggtg cgccgctctc tcgcctcgcg acgctcgaaa
     2041 aagctccgca ggagctgcgc aacgtgcgag tctcccgccg cgagcctctc gagcggacgc
     2101 ccgagctgtc gagcgcgatc gaaagcgcaa gcgcgaccct tggcccgcgt ggacgagtgt
     2161 tcgtgcgcta ctcggggac
//
LOCUS       DASWAV010000674         5345 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U2665
            k141_438750, whole genome shotgun sequence.
ACCESSION   DASWAV010000674 DASWAV010000000
VERSION     DASWAV010000674.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960316
            Sequence Read Archive: SRR10260007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 5345)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5345)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:47:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,929
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5345
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_438750"
                     /isolate="SMAG_U2665"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.36444 N 132.31889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            172..1791
                     /locus_tag="VF958_14185"
     CDS             172..1791
                     /locus_tag="VF958_14185"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024118.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HEX9150301.1"
                     /translation="MSGTGELSIVALGGLGEFGRNVLWLSSGDSNVVIDAGVSFPDEA
                     FPGIDRIAPDLSPLRDVRVDAILLTHGHEDHVGSLPLLSEWCDARVHALPFTVALARR
                     RLEEAGLPTERLVEVRRGEAIRAGAFSFTFFRVSHSVPDSAAILVEAGGWRVFHSGDF
                     KLDADPPDGETTDVEGIRNAAALGVDLALVDSTNAERPGHALSERIAGAGLTGAFANA
                     PGRIILTTFSSHVARISQAAAAAMAQGRRVAILGRSMRTIAEIADRFGRLQIPPASRI
                     AGSELADAAPGRVLCLMGGSQGEPFSALYRLALDEHADLKLASGDLVLFSSRTIPGHE
                     RSVNRVTDHLVRRGARVLRETEPPIHVSGHAHRDDIADWLAMLAPRAVMPVHGETRML
                     AAAAEIARDRGVPGDRVQLLDNGDRLTLSPSGMHVERGAVSTGKIFLDSRPEMVDLEV
                     VRDRRRLAEEGFVVVLVADSEIAIVSRGVARDEAGLTGEISRAARAVLDRATPEERAD
                     VEWLRAEIAIAAKRACRREFGIRPVIVPVIV"
     gene            1805..2617
                     /locus_tag="VF958_14190"
     CDS             1805..2617
                     /locus_tag="VF958_14190"
                     /inference="COORDINATES: protein motif:HMM:NF014703.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HEX9150302.1"
                     /translation="MTWLEALLLGVIQGLTEFLPVSSDGHLSAAELLLPHFAQVGVLF
                     DVILHVGTMVAVIVFFRRTLADDAKGFFSKDLSLRRDSFKLAALVVAATIPTGIVGLL
                     LKRAVEVSKRDARFVGAMEIATGLCLLLSFFARDRGRERAETTVFDAMLIGVVQGLAV
                     LPGLSRSATTIAFALLVGLARRWSVTFSFVLLLPAVLGATVLELGSAWRERGPGFFAG
                     PDFVKYLGGAVVAGVVGYFAIGWLIRAVIAKRLHWFAVYCILFGLALILFVR"
     gene            complement(2628..2876)
                     /locus_tag="VF958_14195"
     CDS             complement(2628..2876)
                     /locus_tag="VF958_14195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9150303.1"
                     /translation="MTARRWIEYLAAILAGNAIYFLVLFPSLPPVLQHQAPDPRSPVP
                     FRFDGGLVLAFLCCVIVYWVIRLASRHAHRLNQRSAMR"
     gene            2972..4399
                     /locus_tag="VF958_14200"
     CDS             2972..4399
                     /locus_tag="VF958_14200"
                     /inference="COORDINATES: protein motif:HMM:NF014385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HEX9150304.1"
                     /translation="MDPEASGGINAQLGRLARGGAVLLGVFLVAGASPVSGSADEPAG
                     ALPAPSTTAPLTLAEAVALAARNNELSGIATARLDRARALRRQAYSALLPDFSFAGTY
                     TRRSSEVTRIVDDEKIVVQAIDAFSGVATIETNILDVRAFPVARSATRNLEAQEHASS
                     EIRRALAFDVASSFFAILSAERLREAASRRIEVAQATVSDARTRLEAGLASSNDLTRA
                     ELELATAQLALTQAQNAVVDSRLSLGYLIAAPVVDRPLADPPAEPADSASPEELEQVA
                     QEARADLHELTLRAESLRLLALEPELRVIPTLTLRGTYRGTNEAGLSGRERDWNVAAL
                     LNWEIFDGGTRYAEGAARRAEYREALLNADALRRRIGLEIRTARTDLATARASLDQAE
                     VRARVAEQNAREVRVRYSEGLATALEQADATVSAFEAAAELARQRFALGIAQLSLSRA
                     LGRWPVAATTPPEPGGAGTSERLSP"
     gene            4396..>5345
                     /locus_tag="VF958_14205"
     CDS             4396..>5345
                     /locus_tag="VF958_14205"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HEX9150305.1"
                     /translation="MKSGGIGKRAGTTLSGAVLLLLLPLGCSKDAESGAGGSGAAAGG
                     RGRGEIEFPVEVARVEARSVEYAVSAVGSVEAFERVEITSRVPGAIDRIHFTEGSLVG
                     PGTLLVEIEPERYRLAVRSAQATLEKAQATGAEAQIGLDRRIAVNAKNPALVKEEDLD
                     AWRTRVSTAKAEVAQARSALDLAQLNLREAFVRAPAAGVIQTRNVQTGQYVQPGVTLA
                     TLVRREPLLLRFQVPEQDASRLTPGMRARFKVRESTRQYTATLTHVAASAESTSRMVA
                     ITAQVDDPSRGELRPGSFAQVTVPVGSSAEAAVIPQTAVRP"
BASE COUNT          710 a         1883 c         1887 g          865 t
ORIGIN      
        1 ctcgcttgcc ttccgcttcc gccgcaagac agtcgcgaat gccctctctt ccgcggtgga
       61 ccgggcgcgg gtcacccggg cgctcgagga gctcggccgc agtcccatgg cccgggccga
      121 ggagctctcg ctcgccgact atgtggcgct ggccgctcga atcggagccc catgagcggg
      181 acaggcgagc tctcgatcgt cgcgctcggc ggcctcggcg agttcggacg caacgtcctc
      241 tggctgtcct ccggcgactc gaacgtcgtc atcgacgccg gcgtttcctt tcccgacgaa
      301 gccttcccgg ggatcgatcg gatcgctccg gacctgtcgc cgctgcgcga cgttcgcgtg
      361 gacgcgatcc ttctcaccca tggccacgag gaccacgtcg ggtcgctgcc gctcctgtcg
      421 gagtggtgtg acgcgcgagt ccatgcgctg ccgttcaccg tggcgctcgc gcgtcggaga
      481 ctcgaggagg ccgggcttcc gacggagcgg ctcgtcgaag tgcgccgcgg agaggcgatc
      541 cgcgccggcg cgttctcgtt caccttcttc cgggtctcgc actcggttcc cgactccgcg
      601 gcgatcctgg tcgaagccgg agggtggcgg gtttttcact ccggcgactt caagctcgac
      661 gcggaccctc ccgacggaga gacgaccgac gtcgaaggga tccggaatgc cgccgccctc
      721 ggggtcgatc tcgccctcgt cgactcgacc aacgccgaga ggccgggcca cgccctttcc
      781 gagcgcatcg ctggggccgg gttgacgggg gccttcgcca acgccccggg ccggatcatt
      841 ctcacgacct tctcgagcca cgtcgctcgg atctcgcagg cggcggcggc cgcgatggcg
      901 cagggccgcc gagtcgcaat cctcggccgc agcatgagga cgatcgccga gatcgcggac
      961 cgtttcggcc gcctccagat tcccccggcc tcccggatcg cgggttccga gctcgccgac
     1021 gcggcgcccg gaagggtgct gtgcctgatg gggggaagcc agggcgagcc gttctcggcg
     1081 ctctaccggc tggcgctgga cgagcacgcc gatttgaagc tcgcttcggg cgacctcgtc
     1141 ctgttctcgt cgcgcacgat ccccggccac gagcgctcgg tcaaccgggt gaccgaccac
     1201 ctggtccgcc gcggcgcgcg agtgctccgg gagacggagc cgccgatcca cgtctccggg
     1261 cacgcgcatc gcgacgacat cgccgactgg ctcgcaatgc tggcgccacg cgccgtgatg
     1321 ccggttcacg gcgaaacgcg gatgctcgcc gcggcggcgg agatcgcgcg cgatcgcgga
     1381 gttcccgggg accgcgtgca gctgctcgac aacggcgacc gcctgactct ctccccgtcg
     1441 gggatgcacg tcgagcgcgg cgcggtctcg actggaaaga tctttctgga ctcgcggccg
     1501 gagatggtgg atctcgaggt cgtccgcgac cggcggcgcc tcgcggaaga gggtttcgtc
     1561 gtcgtgctcg tggcagacag cgagatcgcc atcgtgtcgc ggggagtcgc ccgggacgag
     1621 gcggggttga cgggcgagat ctcgcgcgcc gcccgggccg ttctggaccg ggcgacgccg
     1681 gaggagcggg cggacgtcga gtggcttcgc gccgagatcg cgatcgccgc caagcgcgcc
     1741 tgccgccggg agttcgggat ccgtccggtg atcgtcccgg tgatcgtctg agcggggaag
     1801 ccggatgacc tggctcgaag cgctgcttct cggcgtgatc caggggctca ccgagttcct
     1861 gccggtgtcc tcggacgggc atctctccgc cgccgagctc ctgctgccgc acttcgcgca
     1921 ggtcggcgtg ctcttcgacg tgatcttgca cgtcgggacg atggttgccg tcatcgtgtt
     1981 ctttcgccgg acgctcgccg acgacgcgaa aggattcttc tcgaaagacc tgagtctccg
     2041 gcgcgactcg ttcaagctcg ccgctctcgt cgtcgccgcg accattccca ccggcatcgt
     2101 cggactgctt ctgaagcggg ccgtcgaggt ttcgaagcgc gacgcgaggt tcgtcggcgc
     2161 aatggagatc gcgaccgggc tctgccttct gctttctttc ttcgcccggg accgcgggcg
     2221 cgagcgggcg gagacgacgg tcttcgacgc catgctgatc ggggtcgtcc agggtctcgc
     2281 ggtccttccc ggtctctccc ggtcggccac cacgatcgcg ttcgcccttc tcgtggggct
     2341 cgcgcgccgc tggtcggtga ccttttcttt cgtcctcctg cttccggccg tcctcggcgc
     2401 gacggtcctc gagctcggga gcgcctggcg cgagaggggg cccggcttct ttgcggggcc
     2461 ggacttcgtg aagtacctcg gcggagcggt cgtcgcgggg gtcgtcggct atttcgcgat
     2521 cgggtggttg attcgggccg tcatcgcgaa gcggcttcac tggttcgcgg tctactgcat
     2581 tcttttcgga ctggcgctga tccttttcgt ccggtgagtg agggtctcta ccgcatcgcg
     2641 ctgcgctggt tcaggcggtg agcgtggcgg ctcgccagcc ggatgaccca gtagacgatc
     2701 acgcagcaga ggaaggcgag aacgaggccg ccgtcgaacc ggaaggggac cgggctcctc
     2761 ggatccggag cctggtgctg gaggaccggc ggcagactcg gaaagagcac gagaaagtag
     2821 atcgcgttgc ccgccaggat cgctgcgagg tattcgatcc agcgtcgcgc cgtcacgcct
     2881 gcagtctacc gccgactcca cgcgcgggaa tgcgccccgc cttcgggcgt ggcccggtct
     2941 aagattccgt tcttcagcgc acggaggagt cttggacccg gaggcgtcgg gtgggatcaa
     3001 cgcacagctc ggccggctcg cgcggggagg cgcggtcctg ctcggcgttt ttctcgtcgc
     3061 cggcgcgtct ccggtttcgg gatccgcgga tgagcctgcc ggcgcgcttc ccgcgccgtc
     3121 gacgacggct ccgctgacgc tcgccgaagc cgtcgccctc gctgcgcgca acaacgagct
     3181 ctccgggatc gcgaccgcgc gtctcgaccg cgcccgggcg ttgcggcggc aggcctactc
     3241 ggcgctcctg ccggacttct cgttcgccgg aacctacacg cgccgctcga gcgaggtcac
     3301 ccggatcgtc gacgacgaga agatcgtcgt ccaggcgatc gacgcgtttt ccggggtcgc
     3361 gacgatcgag acgaacattc tggacgtgcg cgcgtttccc gtcgcccgca gcgccacccg
     3421 gaatctcgaa gcccaggagc acgcttcgtc ggagatccgg cgcgcgctcg cgttcgacgt
     3481 cgcctcgagc ttcttcgcaa tactgtccgc ggaacggctg cgggaggccg cgagccgccg
     3541 catcgaggtc gctcaggcga ccgtctccga tgcccgcacg agactcgagg cggggctcgc
     3601 ctcgtccaac gacctgacgc gcgcggagct cgagctcgcc accgctcagc tcgcattgac
     3661 gcaggcccag aacgccgtcg tcgactcccg actttcgctc ggatacctga tcgccgcacc
     3721 ggtcgtcgat cggccgctgg ccgacccgcc ggccgaaccg gcggactccg cgagccccga
     3781 ggagctcgag caggtcgcgc aggaggcgcg ggccgacctg cacgaactca ctcttcgcgc
     3841 cgaatcgctg cggcttctcg cgctcgagcc ggaactgcgg gtgattccga cgctgacgct
     3901 gcgcgggacc tatcgcggga cgaacgaggc gggcctttcc ggtcgcgaac gcgactggaa
     3961 cgtcgccgct ctcctgaact gggagatatt cgacggaggc acgcgctacg cggagggagc
     4021 ggcgcgccgc gccgagtacc gcgaggcact tctgaacgcc gacgccctcc ggcgccgaat
     4081 cggcctcgag atccgcacgg cgcggaccga cctcgcgacc gcccgcgcgt cgctcgatca
     4141 ggcggaggtc cgcgcgcgcg tggcggaaca gaacgcgcga gaggtgcgcg ttcgctactc
     4201 cgaggggctc gcaaccgcgc tcgagcaggc ggacgcgacg gtttccgctt tcgaggccgc
     4261 ggccgagctc gcccggcagc ggttcgcgct cggaatcgca cagctctcgt tgtcccgcgc
     4321 gctcgggcgg tggccggtgg cggcgaccac tccgccggag cccggaggcg cagggacgtc
     4381 cgagcgcctt tcgccatgaa atccgggggg atcggcaagc gcgcggggac gaccctttcg
     4441 ggcgcggtcc tcctccttct ccttcctctg ggctgctcga aggacgccga gtcgggcgcg
     4501 ggggggagcg gcgccgctgc gggggggcgc gggcgcgggg agatcgagtt cccggtcgag
     4561 gtcgcgcggg ttgaggcgcg ctcggtcgag tacgcggtct cggcagtcgg ctccgtcgag
     4621 gcgttcgagc gcgtcgagat cacctcccgc gtgcccggcg cgatcgaccg gatccacttc
     4681 acggaaggaa gcctcgtcgg cccgggcact ctcttagtcg agatcgaacc cgagcgctac
     4741 cggctcgccg tccgctccgc gcaggccacg ctcgagaaag cgcaagcgac cggggccgaa
     4801 gcgcagatcg gtctcgaccg gcgaattgcg gtcaatgcga agaaccccgc gctcgtgaag
     4861 gaggaggatc tggacgcatg gcgcacccgg gtctccaccg cgaaagcgga ggtcgcacag
     4921 gcgcgttccg ccctcgacct cgctcagctc aatctgcgcg aggcgttcgt ccgggcgccc
     4981 gcggccgggg tgatccagac ccggaacgtc cagaccggtc agtacgttca gcccggcgtg
     5041 acgctcgcga cgctcgtgcg ccgcgagccg ctcctgctgc gcttccaggt ccccgagcag
     5101 gacgcgtcgc ggctcacccc cggcatgcgc gcgcgcttca aggtccgtga gtccacccgg
     5161 cagtacacgg cgactctcac tcacgtcgcg gcgtcggccg agtcgacgtc gcggatggtg
     5221 gcgatcaccg cgcaggtgga cgatcccagc cgcggcgagc tgcggccggg ctcgttcgcg
     5281 caggttacgg tgccggtcgg ctcctccgcg gaagcggccg tgatacccca gaccgccgtc
     5341 cggcc
//
LOCUS       DASWAV010000675         4678 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U2665
            k141_799142, whole genome shotgun sequence.
ACCESSION   DASWAV010000675 DASWAV010000000
VERSION     DASWAV010000675.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960316
            Sequence Read Archive: SRR10260007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 4678)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4678)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:47:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,929
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4678
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_799142"
                     /isolate="SMAG_U2665"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.36444 N 132.31889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1151)
                     /gene="dnaG"
                     /locus_tag="VF958_14210"
     CDS             complement(<1..1151)
                     /gene="dnaG"
                     /locus_tag="VF958_14210"
                     /inference="COORDINATES: protein motif:HMM:TIGR01391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of RNA
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HEX9150306.1"
                     /translation="MSDLDINDNVLAELRSAADIVEVVGEHTRLKKAGRSWKGLCPFH
                     NERTPSFTVDRDKGLYHCFGCGAGGDVIHFVRQIDRLDFPEAIEALASRFGVAIPKRV
                     SRGPRDEQREKLLAAVAAAQRFYAAELAKPGNRAAEYLEERAVPEPVARSLGVGYAPA
                     GWETLAKGLGGAWPESLLVEAGLLQPRSEGRGSYDRFRNRLVFTIRDERGRPVGFSGR
                     ALSPEDEPKYLNSPESPIFLKKRLLYGLTEAREAIRRRERTVLAEGCFDHLALYLSGV
                     EEAVASMGTALTPEQAAKLKRLAPRVVVCYDGDPAGRNAARAALSQLLAQGLEVSVAR
                     LPADEDPDDVRRRDGLEALARRIDEAPGYLPWLLEELRPAEPRVSAEEK"
     gene            complement(1155..3323)
                     /locus_tag="VF958_14215"
     CDS             complement(1155..3323)
                     /locus_tag="VF958_14215"
                     /inference="COORDINATES: protein motif:HMM:NF012700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Smr/MutS family protein"
                     /protein_id="HEX9150307.1"
                     /translation="MLESSGGAASPADFRPIVGSARAAQAVRRALAGVKTPQLAERAA
                     RLPDLQSLVDASARIIGPDGAVRDDASTELAALRRRLRRRRSDVAGRLEKMLETRRDA
                     LADSVVVLRNDRYCLPVAASSRSRVAGIVHDRSGSGQTVFVEPLEVVEANNELALAAA
                     EEKREVERLLTALGRQMLERADDLIAATEEVAELDGREAAVDFAALCDARVPEISDDG
                     SWTISGARHPLLDPRLADLRRRALGETRAAKEAIPLDLDLEPAQRLLVVSGPNAGGKT
                     VVLKTAGLFSLLAQAGLPVPANPGTRLPVFGAISTEIGDAQQILADRSTFSSSMETLT
                     SMLETAAPDRLALIDEIGSATDPEEGGALAVAFLEEYLAAGGRAIVTTHLSAIKAFTA
                     ARSDAVGAAMEFDEKTGRPTYRLHPGLSGRSRALSVAERQGLPPRVLARAREILGAAW
                     ERRDRAESDAEQALERLRVGEEELSRARQEARRETERLSAEREAVARERERMLEAGLG
                     GFEKAKKDLLHRVEVEIEAIREDAARRASESAAQVLERAEAESETEVVGEAREDRIAR
                     SRAIASGDRVRIRGARSEGVVASIEGETAWLEVGGKRMRVPLGELERIGDSAASAPSP
                     RPSPGGRGKSDAEVELHGFTPEVNVIGRRLDEAIEEVEKALDAALAAGADRFRVVHGH
                     GTGRLRDGLRDHLRKHPAVSRLRAADPREGGNGATIVELA"
     gene            complement(3566..3772)
                     /gene="rpsU"
                     /locus_tag="VF958_14220"
     CDS             complement(3566..3772)
                     /gene="rpsU"
                     /locus_tag="VF958_14220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005033802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HEX9150308.1"
                     /translation="MPLVHVKEDESFENAMRRFKRKCEKSGILSELKKRQHYEKPSAK
                     RKRKAIQARKKMLRKLSEERRATM"
     gene            4099..>4678
                     /locus_tag="VF958_14225"
     CDS             4099..>4678
                     /locus_tag="VF958_14225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9150309.1"
                     /translation="MGDHDSAGAPTSRELRARLRERLSALKGDLERLRFETDRRWDEL
                     LTRLSERPEEIVPEELLREASRPESPGAISAEAARRIDSATTQVEALTFFVEECRRHA
                     SRVALLVERGGRFEIWRSSGFAAGEARGKSLSADAGGELREGIPQRLGRGSAVSALLG
                     AADASEAVLVPFVVREKVSGAVYADAVSGNRDR"
BASE COUNT          678 a         1630 c         1612 g          758 t
ORIGIN      
        1 ttcttttctt ccgccgaaac ccgaggctcc gccggccgga gctcctcgag cagccacgga
       61 agatatcccg gcgcttcgtc gatccttcgc gcgagcgcct ccaggccgtc ccggcggcgg
      121 acgtcgtcgg gatcctcgtc ggcgggaagg cgggcgacgg agacttccag cccctgcgcc
      181 aacagctgcg agagcgccgc gcgcgcggca ttccggcccg cggggtcgcc gtcgtagcag
      241 acgacgactc gcggcgcgag ccgcttcagc ttcgccgcct gctcgggggt cagcgccgtg
      301 cccatcgagg cgaccgcctc ctccactccg gagagataga gcgcgaggtg gtcgaagcaa
      361 ccttcagcca ggacggtcct ctcgcgccgg cgaatcgcct cgcgtgcttc agtgaggccg
      421 tagaggagcc tcttcttcaa gaagatcggc gactccggag agttcagata ttttggctcg
      481 tcctcgggag agagcgcgcg gccactgaag ccgacggggc ggccacgctc gtcccggatg
      541 gtgaaaacga ggcggtttcg gaaacggtcg tacgatccgc gcccctccga gcgcggttgc
      601 agcagacccg cttcgacgag caggctctcc ggccacgcgc cgccgaggcc cttcgcgagc
      661 gtctcccagc cggcgggggc gtatccgact ccaagagacc gggcgacggg ctcgggaacg
      721 gcgcgctcct cgagatactc ggccgcccgg tttccgggct ttgcgagctc cgcggcgtag
      781 aagcgctgcg cggcggcgac cgcggcgagg agcttctcgc gctgctcgtc tcgggggccg
      841 cgcgagacgc gcttcggaat tgcgacgccg aagcgagatg cgagcgcctc gatcgcttcg
      901 gggaagtcca gccggtcgat ctggcgcacg aaatggatca cgtcaccgcc ggctccgcac
      961 ccgaagcagt ggtacagccc cttgtcccgg tcgaccgtga atgatggcgt gcgctcgttg
     1021 tggaacgggc acaatccctt ccaggagcgg cccgccttct tcaagcgggt gtgctcgccg
     1081 acgacctcga cgatgtcggc cgcgctgcgc aattccgcga gcacgttgtc gttgatgtcg
     1141 aggtcagaca tcgctcacgc cagctccacg atggttgcgc cattgccccc ttcgcgcgga
     1201 tcggcggcgc gaagccggga aacggcggga tgctttctca gatgatctct caatccgtcg
     1261 cgcaggcgcc ccgtgccgtg cccgtgaacg acgcggaagc gatcggcgcc ggcggcgagc
     1321 gccgcgtcga gggccttctc gacctcctcg atggcctcgt cgagccggcg cccgatgacg
     1381 ttcacttcgg gggtgaaacc atgaagttcg acttccgcgt cggacttccc tctccctccg
     1441 ggagagggcc ggggcgatgg ggctgaggca gccgagtcgc cgattcgctc cagctccccg
     1501 aggggcaccc gcatcctctt tccgccgacc tcgagccacg cggtttcccc ttcgatcgat
     1561 gcgacgacgc cctccgagcg cgccccgcga atcctcacgc ggtcgccgga tgcgatcgcc
     1621 cgcgagcggg cgatgcgatc ttcgcgcgct tcgccgacga cctcggtctc cgactcggcc
     1681 tcggcgcgct cgaggacctg cgcggcggac tcgctcgcgc gacgcgcggc gtcctcgcgg
     1741 atcgcttcga tctccacctc cacgcggtgc aggaggtcct tcttcgcctt ctcgaaaccg
     1801 ccgaggcccg cctccagcat tcgttcccgc tcgcgcgcga ccgcctcacg ctcggcggag
     1861 aggcgttccg tctcgcgccg cgcttcctgg cgcgcgcgcg acagctcctc ctcgccgacg
     1921 cgcagccgct cgagcgcctg ttcggcgtcc gactccgccc ggtcgcgacg ctcccacgcc
     1981 gcgccgagga tctctctcgc gcgagcgaga actctcggcg gcaagccctg ccgctccgcg
     2041 acggagagcg cgcgactgcg ccccgaaaga cccgggtgca ggcgataggt cgggcgacct
     2101 gtcttctcgt cgaattccat cgccgcgccg accgcgtcgg agcgcgccgc cgtgaacgct
     2161 ttgatcgcgg agaggtgggt cgtgacgatc gcgcgcccgc ccgcggcgag gtactcctcg
     2221 aggaaagcga ccgcgagagc gcccccctcc tcggggtccg tcgcgctgcc gatttcgtcg
     2281 atcagcgcga gccggtcggg cgcggcggtc tcgagcatgc ttgtgagcgt ctccatcgag
     2341 gaggaaaacg tcgagcggtc cgcgaggatc tgctgtgcgt cgccgatctc ggtcgagatc
     2401 gcgccgaaca ccggcaggcg cgtgccggga ttcgcgggaa ccggcagccc ggcctgcgcg
     2461 aggagcgaga agaggccggc cgtcttcagg acgacggttt ttccaccggc gttcggaccc
     2521 gacacgacga ggaggcgctg cgccggctcg aggtcgaggt cgagcggaat cgcctctttg
     2581 gccgcgcgcg tctcgccgag cgcacggcgc cgaaggtcgg cgaggcgagg gtcgagaagc
     2641 gggtgccggg cgcccgagat cgtccagctc ccgtcgtccg agatctccgg cacgcgcgcg
     2701 tcgcacagcg ccgcgaagtc gaccgcggcc tcgcgcccgt cgagctccgc gacctcttca
     2761 gtcgcggcga tcaaatcgtc cgcgcgctcg agcatctgcc ggccgagggc tgtcaggagc
     2821 cgctcgacct cgcgtttctc ttcggccgct gcgagcgcca gctcgttgtt ggcttcgacg
     2881 acctcgagcg gctcgacgaa cacggtctgt ccggagcccg agcggtcgtg cacgattccg
     2941 gcgacgcggg atcgcgacga cgccgcgacc ggaaggcagt agcggtcgtt tcggagtacg
     3001 acgacggagt ccgcgagcgc gtcgcgacgc gtctcgagca tcttttcgag ccggccggca
     3061 acgtcggaac ggcggcgtcg cagccgacgg cgcaacgccg cgagctcggt cgaggcatcg
     3121 tcacgtaccg caccgtccgg tccaatgatc cgcgcggacg cgtcgacgag agactgaagg
     3181 tcgggcagcc tcgcggcacg ttcggcgagt tgcggcgtct tgacacctgc aagcgcgcgc
     3241 cggaccgcct gcgccgcgcg ggccgacccg acgatcgggc ggaagtcagc cgggctcgcg
     3301 gcgcctccgg agctctcgag catcgcgagc ggcgcttcga ggtcgacgag gcccgtgagc
     3361 gggagtcgtc cttcgcgcgc gcgaaaatcg aggagctcgg agagctcgtc gagacgacgg
     3421 cggaccgacg cctcatccca ggaaggttgc gtttggagaa cggcctcccg gccgggaggc
     3481 gtgcgggcaa gactggaaac cagctggagg acgacgtcga attcgagttc gcggtaatcg
     3541 acgccgtcct ccgccgaatg actgactaca tcgtcgcccg acgctcctcc gagagtttgc
     3601 ggagcatctt cttgcgcgcc tggattgcct tgcgcttccg tttcgcgctc ggtttctcgt
     3661 agtgctgacg tttcttcaat tcggagagga tgcccgactt ctcgcacttg cgcttgaagc
     3721 gtcgcatcgc gttctcgaag gactcgtctt ccttgacgtg gacgagaggc acgaacattc
     3781 acccccttct ccgtgggttt gggtcgatcg gccatgttac tgcggcgacc ccatgctgtc
     3841 aatgaaatgc tttgtgcgca aagacttagc tgctgattgc ctcctcggga gccgctcggc
     3901 ccgatcgtcc gcgcgcgctt cccaacgcgg ccgcccggcg agtccttcct caaatttctc
     3961 tttccgccgg ggtgctgccg ggcacggcgc agcgcagcgc cttcgcagct gcggccgcgg
     4021 tctcgtgtca caagttgcgg gttggaaaag gtttagccgt tgactgcatc cgaaagtcgc
     4081 ccggataatc aaactgctat gggggaccac gattcggccg gcgcccccac ttcccgggag
     4141 ctccgggcgc gcctgcgcga aaggctttcc gcgctcaaag gcgacctcga gaggttgagg
     4201 ttcgagaccg accggcgatg ggacgagctg ctcacgcggc tttccgagag acccgaggaa
     4261 atcgtgccgg aggagcttct ccgggaggcc tcgcgtccgg agagccccgg agcgatctcc
     4321 gccgaagccg cgcgtcggat cgattctgcg acgacgcagg tcgaggcgct gacctttttc
     4381 gtcgaggagt gccggcgcca cgcctcgcgc gttgcgctgc tcgtcgagcg gggcggacgg
     4441 ttcgagatct ggagatcctc cggattcgcc gccggtgaag cgagggggaa gagcctctcc
     4501 gccgacgccg gcggggaatt gcgcgagggg attccgcaac gcctcggtcg ggggagcgcg
     4561 gtctccgccc ttctcggcgc cgccgacgcg tcagaagccg tgctcgtgcc gttcgtcgtg
     4621 cgggagaagg tgtccggcgc ggtgtacgcg gacgccgtgt cgggaaaccg cgaccgcc
//
LOCUS       DASWAV010000676         2132 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U2665
            k141_119634, whole genome shotgun sequence.
ACCESSION   DASWAV010000676 DASWAV010000000
VERSION     DASWAV010000676.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960316
            Sequence Read Archive: SRR10260007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2132)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2132)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:47:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,929
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2132
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_119634"
                     /isolate="SMAG_U2665"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.36444 N 132.31889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..456)
                     /gene="thpR"
                     /locus_tag="VF958_14230"
     CDS             complement(<1..456)
                     /gene="thpR"
                     /locus_tag="VF958_14230"
                     /inference="COORDINATES: protein motif:HMM:NF024952.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008664 - 2'-5'-RNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2',3'-cyclic phosphodiesterase"
                     /protein_id="HEX9150310.1"
                     /translation="MRVFLVVPSDPLWVDSARGLMASLRNGLPHASWTRAESWHVTLL
                     FLGEVGGGFVSRYAGAVETAALETVAGEIQAGPPAVFPERGRPRVLGVGFSPSPGVES
                     VTRLAGAAHDAAVRLGWEEASRRPFHPHVTFARVRESWPEPAVADYRRAV"
     gene            complement(453..1706)
                     /locus_tag="VF958_14235"
     CDS             complement(453..1706)
                     /locus_tag="VF958_14235"
                     /inference="COORDINATES: protein motif:HMM:NF001813.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence/damage-inducible protein A"
                     /protein_id="HEX9150311.1"
                     /translation="MSTVSPRAAIIAIGSEMLGTLRRDTNSLWLTGRLEEAGFTVVRK
                     SIVGDDPGEISRELSCASSAAAWIFTTGGLGPTADDLTVAAVARWAGRGVRRDEEFVA
                     AMRRRWERRGVPMPAVNEKQADFIEGARVLANPRGTAPGFWLDVEGVQVLILPGVPSE
                     MKEIFGESIEPELVSSSAITTRRRVLRIAAMAESAVEELVAPLYAKWSSDPVTILASP
                     GEVELHLSARGTPAEAAARLEEMEDDFVRVLGSRIHGRDDEGLAATVGRLLTGAGKTV
                     ALAESCTGGLASTLLTDVPGSSAYFLGSVVSYADSAKESILDVSGDTLRDHGAVSADA
                     AREMARGALARFGSDLAAAITGIAGPDGGSEAKPVGTVFFALVDRGGRESAKKRVYGG
                     DRATVRRASAMFTLELIRRFLTEAK"
     gene            complement(1703..>2132)
                     /locus_tag="VF958_14240"
     CDS             complement(1703..>2132)
                     /locus_tag="VF958_14240"
                     /inference="COORDINATES: protein motif:HMM:NF027650.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9150312.1"
                     /translation="RVLDLRAGRARLYDGNYTDTAEAREDRRRKPEADAATVVSPQSS
                     VLSPPKPVVRARPPASEQEKEGARRKRRIEALEEKIAACERQIEAIETRLWEEGLDLG
                     PVESHRLATEKAARREELEKLVEDWAKLSEEVEPAGSTVP"
BASE COUNT          316 a          775 c          718 g          323 t
ORIGIN      
        1 gacagcccgg cggtagtcgg ccaccgcggg ttcgggccag ctctcgcgaa cccgtgcgaa
       61 ggtgacatgc ggatggaatg gtctgcggct cgcctcctcc cagccgagcc gcaccgcggc
      121 gtcgtgcgcg gcgccggcaa gccgggtgac tgactcgacg cccggcgagg gcgagaaacc
      181 gacaccgagc acacgggggc ggccgcgctc cggaaaaacc gccggcggcc cagcctggat
      241 ctctccggcg accgtctcca aggcggcggt ttcgaccgcg ccggcatatc ggctcacgaa
      301 tccccccccg acctctccga gaaagagaag cgtcacgtgc cagctctcgg cccgggtcca
      361 ggaagcgtgc gggaggccgt tgcggagcga cgccatgagc ccgcgggcgc tgtcgaccca
      421 caggggatcg gagggaacca caaggaagac cctcatttcg cctcggtcag gaagcgccga
      481 atcagttcga gcgtgaacat ggcggaggcg cgccggaccg tcgctcgatc tccgccgtag
      541 acgcgtttct tcgccgactc gcggccgccg cggtcgacga gcgcgaagaa gaccgttccg
      601 accggctttg cttccgagcc gccgtcgggg ccggcgattc cggtgatggc ggcggcaaga
      661 tccgatccga atcgggcgag cgcgccgcga gccatctctc gcgccgcgtc cgcggagacg
      721 gcgccgtgat cccgaagggt gtcccccgat acgtcgagga tgctctcctt ggccgaatcc
      781 gcgtaggaga cgaccgatcc gaggaaataa gcgctggatc ccggcacgtc cgtcaagagc
      841 gtcgacgcga gacctcccgt acacgattcc gcgagcgcga ccgtcttgcc cgcgccggtc
      901 aggagacggc cgacggtcgc ggcgagtcct tcgtcgtccc ggccgtggat gcgagatccc
      961 aggacccgca cgaagtcgtc ctccatttcc tcgaggcggg ccgccgcctc cgccggcgtg
     1021 ccccgggcgc tcaggtggag ctcgacctcg cccggcgagg ccaggatcgt gaccgggtcg
     1081 gagctccact tcgcgtaaag gggcgcgacg agctcttcga ccgccgactc ggccatcgcg
     1141 gcgatgcgga gaacccgccg tcgggtcgtg atcgcgctcg atgagacgag ctccggctcg
     1201 atcgactcgc cgaagatctc cttcatttcg gagggcacac cgggcaggat caggacctgg
     1261 acgccctcga cgtccagcca gaacccgggc gcggtcccgc gcgggttcgc gagaacgcgg
     1321 gccccttcga tgaagtcggc ctgcttctcg ttcaccgcgg gcatggggac gccgcgccgc
     1381 tcccagcgcc gccgcatcgc ggcgacgaac tcttcgtcgc gccggacgcc tcggcccgcc
     1441 cagcgagcga ccgcggcgac ggtcaggtca tcggcggtcg gaccgaggcc gcccgtcgtg
     1501 aagatccacg cggctgcgga cgatgcgcac gagagctcgc gcgagatctc gcccggatcg
     1561 tcgccgacga tcgacttgcg gacgaccgtg aagccggctt cctccagccg gcccgtcagc
     1621 cagagcgagt tcgtgtcccg ccgcagcgtc ccgagcattt cgctgccgat cgcgatgatc
     1681 gcagcgcgcg gcgaaaccgt gctcatggaa ccgtcgagcc ggcgggctcc acctcttctg
     1741 agagcttcgc ccaatcctcg acgagctttt cgagctcctc ccgacgcgcg gccttttccg
     1801 tggcgagccg gtgcgactcg acgggtccga gatcgagccc ctcttcccac aagcgcgtct
     1861 cgatcgcctc gatctgcctc tcgcaagcgg ctatcttctc ctcgagcgcc tcgatccgcc
     1921 gcttgcgccg ggcgccctcc ttttcctgct cggaggctgg aggccgtgcg cgtaccaccg
     1981 gttttggggg actgaggacc gaggactggg gactcacgac tgtcgcggcg tccgcctcgg
     2041 gctttcgtct ccggtcctcc cgcgcttcgg ccgtgtccgt gtagttcccg tcgtacaacc
     2101 tcgcgcgtcc ggctctcagg tcgaggacgc gt
//
LOCUS       DASWAV010000677        11550 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U2665
            k141_119798, whole genome shotgun sequence.
ACCESSION   DASWAV010000677 DASWAV010000000
VERSION     DASWAV010000677.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960316
            Sequence Read Archive: SRR10260007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 11550)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11550)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:47:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,929
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11550
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_119798"
                     /isolate="SMAG_U2665"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.36444 N 132.31889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1179)
                     /locus_tag="VF958_14245"
     CDS             complement(<1..1179)
                     /locus_tag="VF958_14245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005034251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus twitching motility protein PilT"
                     /protein_id="HEX9150313.1"
                     /translation="MLINDLLKIAVDRGASDLHLKVGAHPVIRVDGTLIPLAELRPLM
                     QEETVAMAFSIMSARQKEKFKNSFEIDIAYSVPNLGRFRCNIFQQRGTVGLVLRVIPQ
                     KIRTIKELLLPPVIEKICQEQRGLVLCTGTTGSGKSTTLASMIDLINAQRNEHIMTIE
                     DPIEFLHRDKKSIINQREVDVDTKGFSVALRSALRQDPDVILVGEMRDYETIETALVA
                     AETGHLVLSTLHTLDATETVNRIISVVPPHQQKQIRLQLGAVIRAIISMRLMPRADGL
                     GRVPAVEILVRTGYVRECIENKEKTSLLRDAIQAGTSQYGMQTFDQSLYGLYRGGLIT
                     LEEALRRASNPNEFKLKIQGIQSTSDVAQEQMDSVLEMPTVDFNPVEEESPFDFSNQV
                     P"
     gene            complement(1232..2176)
                     /locus_tag="VF958_14250"
     CDS             complement(1232..2176)
                     /locus_tag="VF958_14250"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020260595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004084 - branched-chain-amino-acid
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009081 - branched-chain amino acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transaminase"
                     /protein_id="HEX9150314.1"
                     /translation="MSYEEVQKVWMNGELVDFADAKIHAFSHVFHYGSAMFEGIRVYK
                     TRNGPAAFRLEDHIERLYHSCKVYRMEIPYTHSQFQDAIFETIRVNGFDACYIRPVIY
                     RGLGALGVNPFKSPVDVIIAVWKWGQYLGEDAVTKGVDVCVSSWNRMAPNTFPAMAKA
                     TGNYLNSMLIKMEAITNGYTEGIALDSAGRLSEGSGENLFLVYQGRILTPPLAGTILP
                     GITRDTVITLAKDAGYIVVETQIAREMLYIADEVFLTGTAAEITPVRSVDRIPVGTGG
                     RGPVTERLQKDFFALLEGEAEDRYGWLTPIPAEVAVSR"
     gene            complement(2223..2942)
                     /locus_tag="VF958_14255"
     CDS             complement(2223..2942)
                     /locus_tag="VF958_14255"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RsmE family RNA methyltransferase"
                     /protein_id="HEX9150315.1"
                     /translation="MRDVRARLIVAGLPAKGEAVALPREESAHVRARRLVRGDAVLLL
                     DGSGRVAEAEIVAGARGETLVRVSRIVEAAANEAPISLLVAGIRAERLSWLVEKATEM
                     GADRITIVTTSRTQAFRASAGALPRLRRIACEAAKQCERNDWPSVEGPVSFERVLATE
                     TGSQRLLLDRDGRAFPRRIEREPAALLVGPEGGFTETERDAASSRGWQVVRLPAGKLR
                     SETAAIAGLVLLRAAGERETG"
     gene            complement(2918..3745)
                     /locus_tag="VF958_14260"
     CDS             complement(2918..3745)
                     /locus_tag="VF958_14260"
                     /inference="COORDINATES: protein motif:HMM:NF018076.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HEX9150316.1"
                     /translation="MSRPKYSRLTVPRRAKDDVVVGVLSLFDPLGFEARGTDLVAYFR
                     DPASARAAGRELDSSRVSHDLVTDIPEEDPFEAYRAASRPFPVGDRLWIDPGDPTESP
                     PPAGRVALRVPASRAFGTGGHESTRLALAAMDEEPLDGLDVLDVGTGSGVLALAAVAL
                     GARRAVGFDSDPQAVFVARENLALHAFGNRIALAACGPEALTGTFPVVVANLLPEEFL
                     PMRTSVCARVAARGRLIVSGIPADREAEVVSRLRSGRWAMAGCRRENGWTCVTFERA"
     gene            complement(3749..4855)
                     /gene="dnaJ"
                     /locus_tag="VF958_14265"
     CDS             complement(3749..4855)
                     /gene="dnaJ"
                     /locus_tag="VF958_14265"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF008035.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HEX9150317.1"
                     /translation="MHSRRDYYEVLGVGRDAGAAELKSAYRKLALQYHPDRNRDDPDA
                     AEKFKEASEAYAVLSDDEKRARYDRFGHAGVGGAGAGGFGGGFDPSIFADFSDLFGDL
                     FGFSGPRDQHASGSDLVYRMEITLKDAAFGVEAPVVVSRLQKCGTCKGSGAEPGSRPV
                     TCPACRGHGRQRISQGFLMITRPCPNCGGDGKVIEKPCRECRGEGRTRTSHKLEIRIP
                     AGVETGSRLRLAGEGDAGPSGGPPGDLYVVLTVMAQEPFEREGENLVLRLEVPFPTLV
                     LGGEIPVPTLEGDQVEITIPPGTQAGTEIKVRGRGLGRLGRSGRGDLVVRAGVAVPEK
                     PSAREKELLRTYAETIGAPVGGETVLDKAKKIFK"
     gene            complement(4860..6695)
                     /gene="dnaK"
                     /locus_tag="VF958_14270"
     CDS             complement(4860..6695)
                     /gene="dnaK"
                     /locus_tag="VF958_14270"
                     /inference="COORDINATES: protein motif:HMM:NF001413.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HEX9150318.1"
                     /translation="MGKIIGIDLGTTNCCVAVVEGSAPQVLANREGSRTTPSIVAFTE
                     DGERLVGQIAKRQAITNPLATVFAVKRLVGRKFDSQETAHAREVLPYEISRAANGDIK
                     IQARGREYSPEEISAFILREIKEFSEEVLGEEINEAIITVPAYFDDAQRQATRDAGRI
                     AGLEVLRIINEPTAAALAYGLDRKGSEIVAVYDLGGGTFDVSILELGDGIYEVKATAG
                     DTYLGGEDFDKKIMDWLLEDFQKSAGIDLKQDRMALQRLKESAEKAKCELSSAMETTI
                     TLPFISADASGPKHINRTLTRDRFEQLVADLIDRTAAPCLDALAAAKLQPSEVDQVIL
                     VGGQTRTPKVGRMVAELFGREPSREINPDEVVAIGAAIQGGVLKGEIKEVVLLDVTPL
                     SLGIETHGGMFEKLIERNSTIPTKNTKVFTTVADNQSVVEIHVLQGERELAKEDKSLG
                     RFELVGIPTAPRGVPQIEVNFAIDSNGIVHVSARDLATGKAQGMQINPAGGLSQSEID
                     RIIHEASAFAAADSERRELAQIRNRLEGMLSSNERVLAEFASALSPDERERIEETLQR
                     SREIADGDSRDALNEALFDMQGVSKVLTRVMLQRAADGAGVRQPS"
     gene            complement(6700..7314)
                     /locus_tag="VF958_14275"
     CDS             complement(6700..7314)
                     /locus_tag="VF958_14275"
                     /inference="COORDINATES: protein motif:HMM:NF013212.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000774 - adenyl-nucleotide exchange
                     factor activity [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_function: GO:0051087 - protein-folding chaperone
                     binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HEX9150319.1"
                     /translation="MIDRGDDDDGDPIEDDRILVEDTGESLDAALAEEERSAIAATEE
                     KLKSVERDLENLKDRHLRSLAEFENLRKRSEREKRDARPLALADLAREFLPVVDSFES
                     ALAHATSEDLESDFGQGVLLIHRQLTDLWRKHGLREVDTAGGFDPNHHEAVTTEPSTA
                     PANSVLEVLRKGYTLGDRLIRPALVKVAVRPDESSAPGGKKREG"
     gene            complement(7307..8374)
                     /gene="hrcA"
                     /locus_tag="VF958_14280"
     CDS             complement(7307..8374)
                     /gene="hrcA"
                     /locus_tag="VF958_14280"
                     /inference="COORDINATES: protein motif:HMM:TIGR00331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcriptional repressor HrcA"
                     /protein_id="HEX9150320.1"
                     /translation="MSNLDTRSRAILKEIIRVHVDTGKPVSSRTLFKSHRFSLSPASI
                     RNIMADLTDAGFLVQPHTSAGRVPTDRAYRLYIDELAHHRRVNEGLRERVDSDLASAG
                     PEMTGIFQAASRLLSRLSGEVGFVVAPDALHTVLKSLRFLAVAPGKVLVVQVNEPDVV
                     LSRVIETAGDYGSRELETASERFTREYAGLTLHEVRRRLVAEMTEEKAAYDRMLTRSL
                     ALGQLALGRGDTEERVYVEGAMKLLDKPEFSDVESLKRIFRAFDEKARLLDLVTRYLD
                     SKETCVVLGSEASLASDPRLSAVLTSFGAGETLTGMLGVIGPARREYPRVIPVVELLG
                     RALTDRLESRDSRARRGNPHD"
     gene            8498..9418
                     /locus_tag="VF958_14285"
     CDS             8498..9418
                     /locus_tag="VF958_14285"
                     /inference="COORDINATES: protein motif:HMM:NF022188.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2520 domain-containing protein"
                     /protein_id="HEX9150321.1"
                     /translation="MPSDVGKTSIALVGPGRAGRAFARSWTAAGGSIVLVARDAGAAR
                     AFARELPRAEVLALDDRLRLACDVLILSVPDDAIGTLARRLSPRVACRFALHFSGALP
                     SAELAPLSSGGTKVASIHPLRAFSGSGEDNWSGAFVAVEGEEAAAKTASRLCRHVGAR
                     PHPLSAANKPLYHAAATLAAGGSGSLLSFATRLWTRAGLAEEDGQSALAGLAAGSVEA
                     VGRLPFDRALTGPVARRDVETIRLHRDALACSPDLLELYALLAGETLRRTPGRGREEE
                     IAGILAREGEPVGDPERESDKRSDGPGRKG"
     gene            9508..10089
                     /locus_tag="VF958_14290"
                     /pseudo
     CDS             9508..10089
                     /locus_tag="VF958_14290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015347803.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase domain-containing protein"
     gene            10882..>11550
                     /locus_tag="VF958_14295"
     CDS             10882..>11550
                     /locus_tag="VF958_14295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011341794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9150322.1"
                     /translation="MTPKTVLLRGRDSVLLCLLVGLFGCAANPRPPAVVPHPSAEYVA
                     MTKASEIFARGREAALAGDFECASEYFRQSIEALRPETGPAPTDPELLSFSFQLYESA
                     LRYEALAGPAEEAGTSDGQMASELNEIAEPPATVEAMVHAKEAVDSDTADLAYDIPIT
                     INEPVLRILAAFQNDERVHSVISRGLSRSGRYMPMIHRIFGEEGIPKDLAQIALIESS
                     FLPHA"
BASE COUNT         1853 a         3962 c         3765 g         1970 t
ORIGIN      
        1 cggcacctgg ttcgagaaat cgaaggggga ttcttcctcg acggggttga agtccacggt
       61 cggcatctcc agaacggagt ccatctgctc ctgcgcgacg tcggacgtcg attgaatccc
      121 ctggatcttc aacttgaact cgttggggtt cgacgcgcgg cggagggcct cctcgagcgt
      181 gatcaggccg ccccggtaaa gtccgtacag agactggtcg aacgtctgca tcccatactg
      241 ggacgtgccg gcctggatcg cgtcgcgcag gaggctcgtc ttctccttgt tctcgatgca
      301 ctcgcgcacg tagccggtgc ggaccaggat ctcgaccgcc ggcacgcggc cgaggccgtc
      361 ggcgcgcggc atcagccgca tcgagatgat cgcgcggatg acggcgccga gctggagccg
      421 gatctgcttc tgctgatgcg gggggacgac ggagatgatc cggttgacgg tctccgtcgc
      481 gtcgagcgta tggagagtcg aaaggacgag gtgacccgtt tccgccgcca cgagcgccgt
      541 ctcgatcgtt tcgtagtctc gcatttcgcc gacgaggatc acgtccgggt cctgccgcag
      601 cgcgcttcgc agcgcgaccg agaacccctt cgtgtcgacg tccacttcgc gctggttgat
      661 gatcgacttc ttgtcgcggt ggaggaactc gatcgggtcc tcgatcgtca tgatgtgctc
      721 gttgcgctga gcgttgatca ggtcgatcat cgaagcgagc gtcgtcgact tgcccgatcc
      781 ggttgtcccg gtgcacagga cgagcccgcg ctgttcctgg cagatcttct cgatgacagg
      841 cggcaggagc agttccttga tcgtccggat cttctgcggg atgacgcgca ggacgagccc
      901 gacggttccc cgctgttgaa agatgttgca gcggaagcgg ccgaggttcg ggaccgagta
      961 cgcgatgtcg atctcgaaac tgttcttgaa cttctccttc tggcgcgcgg acatgatcga
     1021 gaacgccatc gcgacggtct cttcctgcat gaggggccgc agctcggcca gcgggatgag
     1081 cgttccatcg acacggatga cggggtgggc tccgactttc aggtggaggt cggacgcgcc
     1141 gcggtcgacc gcgattttca gcagatcgtt gatcagcacg gatttatcct cagaccctta
     1201 agggtacgcc cgaagcccgg agattggaag tctaccgact cacggcgacc tccgccggta
     1261 tcggcgtcag ccacccgtaa cggtcttcgg cctcgccttc caggagggca aagaagtcct
     1321 tctggaggcg ctcggtgaca ggcccgcggc cccccgtacc gaccgggatg cgatcgacgg
     1381 accggacggg cgtgatctcc gcggcagtcc cggtcaggaa cacctcgtcg gcgatgtaga
     1441 gcatttcgcg ggcgatctgc gtctcgacga cgatgtagcc cgcgtccttc gcgagagtga
     1501 tcaccgtgtc gcgcgtgatg ccgggcagga tcgtcccggc gagcggcggc gtcaggatcc
     1561 ggccctggta gacgagaaag aggttctcgc cgctgccctc ggagagtcgg ccggcgctgt
     1621 cgagcgcgat cccttcggtg taaccgttgg tgatcgcctc catcttgatc agcatcgagt
     1681 tcaagtagtt gccggtcgcc ttcgccattg ccgggaacgt gttcggcgcc atccggttcc
     1741 aggacgagac gcacacgtcc acgcccttgg tcaccgcgtc ctcgcccagg tactgacccc
     1801 acttccaaac cgcgatgatc acgtcgaccg ggctcttgaa gggattgacc ccgagcgcgc
     1861 caagtccccg ataaatgacc ggacggatgt agcaggcgtc gaacccgttc acgcggatcg
     1921 tttcgaatat cgcgtcctgg aactgcgaat gcgtgtacgg gatctccatc cgatagacct
     1981 tgcacgagtg gtagaggcgc tcgatgtgat cttccagccg gaacgccgcg ggtccgttgc
     2041 gtgtcttgta gacgcggatc ccttcgaaca tcgcggagcc atagtgaaag acgtgcgaga
     2101 aagcgtggat cttcgcgtcg gcgaagtcca cgagctcgcc gttcatccag actttctgaa
     2161 cttcctcgta cgacatgcga tgtcctccga tcggcgggcg atccctggcg ggtcgtcgca
     2221 gtctaacccg tctcgcgctc ccccgccgcg cgcaagagta cgagccccgc gatcgccgcg
     2281 gtctccgagc ggagctttcc ggcgggaagt ctgacgacct gccagccgcg ggatgaagcc
     2341 gcgtcgcgct ccgtctccgt gaagccgccc tccggaccga cgaggagcgc cgccggctca
     2401 cgctcgatgc gccgcggaaa agcgcgaccg tcgcggtcga gcaagagccg ctgtgacccg
     2461 gtctcggtcg cgagcacgcg ctcgaacgat acgggacctt cgacgctcgg ccagtcgttc
     2521 cgttcgcact gcttggctgc ttcgcacgcg atccggcgca ggcgcgggag cgcacccgca
     2581 ctcgcgcgga acgcctgggt gcgcgacgtc gtcacgatcg tgatgcggtc ggcgcccatc
     2641 tccgtcgcct tttcgaccag ccacgagagc cgttccgcac ggatgcccgc gacgaggagg
     2701 gagatcggag cttcattcgc agccgcctcc acgatgcgag agaccctcac gagcgtctct
     2761 ccacgggcgc cggcgacgat ctcggcctcg gccacgcggc cgctgccgtc gagcaggagg
     2821 acggcgtcac cgcgcacgag acgccgggcg cgcacgtgtg ccgactcctc tcgcggaagg
     2881 gcgacggctt cacccttcgc cggaagcccg gcgacgatca ggcgcgctcg aacgtcacgc
     2941 atgtccagcc gttctcccga cggcagcccg ccatcgccca gcggccgctc cggagccggg
     3001 agacgacctc cgcctcacgg tcggcgggaa tcccggacac gatgagccgc cctctcgcgg
     3061 ccacgcgcgc gcagacgctc gtccgcatcg gaaggaactc ctccggaagg aggttcgcca
     3121 cgacgacggg aaaggtcccg gtcagagcct cgggtccgca ggccgcgagc gcgatccgat
     3181 tcccgaaggc gtgcagcgcg aggttctcgc gcgcgacgaa gactgcctgc ggatctgagt
     3241 cgaagccgac cgcgcgacgc gcaccgagcg cgaccgcggc gagcgcgagg acgcccgagc
     3301 ccgtcccgac gtcgagcacg tccaggccat ccagaggctc ctcgtccatc gcggcgagcg
     3361 cgagccgcgt cgactcgtgg ccaccggtcc cgaaagcgcg cgatgccgga acgcgcagcg
     3421 cgacgcgccc cgctgggggc ggcgactcag tcgggtcgcc cgggtcgatc cagagacggt
     3481 cacccaccgg aaaagggcgg gacgcggcgc gataggcctc gaacggatct tcttcgggga
     3541 tgtcagtgac gagatcgtgg ctcacccgcg aggagtcgag ctcgcgtcca gccgcgcgag
     3601 cggaggcggg gtcgcggaag tacgcgacca ggtcggtgcc acgcgcctcg aagccgagcg
     3661 gatcgaagag agagagaacg cccacgacca cgtcgtcctt cgcgcggcgc gggaccgtca
     3721 ggcgggagta cttcggacgg gacaatccct acttgaaaat cttcttcgcc ttgtcgagaa
     3781 cggtctcgcc tccgacgggc gcgccgatcg tttcggcata cgttcgcagg agctccttct
     3841 ctcgggcgga gggcttctcc gggaccgcga cgcccgcgcg tacaacgagg tcgccgcgcc
     3901 cgctgcggcc gaggcgtccg agaccgcgcc cccggacctt gatctcggtt cccgcctgcg
     3961 tccccggcgg aatcgtgatc tccacctggt ctccctcgag tgttggcacc gggatctcgc
     4021 cgccgaggac gagggtcggg aaaggcacct cgaggcggag cacgaggttc tcgccttcgc
     4081 gctcgaacgg ttcctgcgcc atcaccgtca gcacgacgta cagatccccc ggaggccctc
     4141 ccgagggtcc cgcgtccccc tcgccggcga ggcgcaggcg tgaccccgtt tcgactccgg
     4201 ccgggatgcg gatctcgagc ttgtgagacg tccgggtgcg cccctcccca cggcactcgc
     4261 ggcacggctt ttcgatcacc tttccgtcgc cgccgcaatt gggacacgga cgcgtgatca
     4321 tgagaaaacc ctgcgagatg cgctggcgcc cgtgcccgcg acacgccgga caggtgaccg
     4381 gccgcgagcc cggctccgcg ccgcttccct tgcacgtgcc gcatttctgg agccgcgaca
     4441 cgacgaccgg cgcctcgacg ccgaacgccg catccttcaa cgtgatctcc atccggtaaa
     4501 cgaggtcgct gccggaggca tgttggtcgc gtggaccgga gaatccgaag aggtccccga
     4561 agaggtcgga gaagtccgcg aagatcgacg ggtcgaaacc gcccccaaac cctccggcgc
     4621 ccgctccccc gacgcccgcg tgcccgaacc ggtcgtagcg cgcacgtttc tcgtcgtcgg
     4681 aaagaacggc gtacgcctcg gaagcctcct tgaacttctc ggcggcgtcc gggtcgtccc
     4741 gattcctgtc ggggtggtac tggagcgcga gcttccgata cgcggacttc aactcggcgg
     4801 cgccggcgtc gcggccgaca ccgaggacct cgtagtagtc gcggcgcgaa tgcatattcc
     4861 taggacggtt gccgcacgcc cgcgccgtcg gccgcccgct gcagcatgac cctcgtcagg
     4921 accttcgaga cgccctgcat gtcgaagagc gcctcgttca gcgcgtcgcg agagtcgccg
     4981 tctgcgatct cccgagagcg ctggagcgtt tcctcgatgc gctcgcgctc gtcgggcgag
     5041 agagcggagg cgaactcggc gagcactcgc tcgttggagg acagcatccc ctcgagccgg
     5101 ttccggatct gcgcgagctc cctccgctcg ctatcggccg cggcgaacgc cgaggcctcg
     5161 tggatgatcc ggtcaatctc cgactgcgac agacccccgg ccgggttgat ctgcatcccc
     5221 tgcgccttac cggttgccag gtcgcgagcc gagacatgca cgatcccgtt cgagtcgatc
     5281 gcgaagttca cttcgatctg cggtacgccg cggggcgcgg tcgggattcc gacgagttcg
     5341 aatcggccga gagacttgtc ctccttcgcc agctcgcgct ccccctgcag gacgtggatc
     5401 tcgacgaccg actggttgtc ggccacggtc gtgaacacct tggtgttctt cgtcgggatc
     5461 gtcgagttcc gttcgatgag tttctcgaac atcccgccgt gcgtctcgat tccgagcgac
     5521 agcggcgtga cgtcgagcag cacgacctcc ttgatctctc ccttgagcac cccgccctgg
     5581 atcgcggcgc cgatcgcgac gacctcgtcg ggattgatct cacgcgacgg ctcgcggcca
     5641 aagagctccg cgaccatccg accgaccttc ggagtgcgcg tctggccgcc gacgaggatc
     5701 acctggtcca cctcggaggg ctggagcttc gcggccgcga gcgcgtcgag gcagggagcg
     5761 gccgtgcggt cgatcaggtc ggcgacgagc tgctcgaacc ggtctcgcgt gagcgtccgg
     5821 ttgatgtgtt tcggcccgga cgcgtccgcg gagatgaacg ggagcgtaat ggtcgtctcc
     5881 atcgcgctcg agagctcgca cttcgccttt tccgcggact ccttcaaccg ctggagcgcc
     5941 attcggtcct gtttcaggtc gattcccgcg ctcttctgaa agtcctcgag gagccagtcc
     6001 atgatcttct tgtcgaagtc ttcgcccccc aggtaagtgt cgccggcggt cgccttcacc
     6061 tcgtagatgc cgtcgccgag ctcgagaatc gagacgtcga acgtgccgcc gcccaggtcg
     6121 tacaccgcga cgatctcgct ccccttccgg tcgaggccgt acgcgagagc cgcagcggtc
     6181 ggctcgttga tgatccggag aacttcgagc ccggcgatcc gtccggcgtc gcgggtggcc
     6241 tgtcgctgcg cgtcgtcgaa atacgcgggc acggtgatga tcgcctcgtt gatctcttcc
     6301 ccgagcactt cctcggaaaa ctccttgatc tcccggagga tgaacgcgga gatctcctcg
     6361 gggctgtact cgcggccgcg cgcctggatc ttaatgtctc cgttggcggc gcgcgagatt
     6421 tcgtagggaa gcacttcccg ggcatgcgcc gtctcctggg agtcgaactt gcggccgacg
     6481 agtcgcttga cggcgaagac cgtggcgaga gggttcgtga tcgcctgtcg cttcgcgatc
     6541 tgtccgacga gcctctcgcc gtcctcggtg aacgcgacga tcgacggagt cgtccggctg
     6601 ccttcacggt tcgcgaggac ctggggcgca gaaccctcga ccacggcgac gcagcagttc
     6661 gtcgtcccga gatcgattcc gatgatcttt cccacggcct cagccctccc gcttcttccc
     6721 gcccggcgcg gagctctcgt ccggacgcac cgcgaccttg acgagcgccg gacggatcag
     6781 gcggtcgccg agcgtgtagc ccttgcgaag gacctcgagc acgctgttcg ccggcgcagt
     6841 cgagggctcg gtcgtgacgg cctcgtggtg gttcggatcg aagccgccgg ccgtgtcgac
     6901 ctcgcgcagt ccgtgcttgc gccagaggtc cgtcaactgg cgatggatca ggagaacgcc
     6961 ctgcccgaaa tcgctctcga gatcctcgga agtggcgtgc gcgagcgcgc tctcgaagct
     7021 gtccacgacc ggcaggaatt cccttgcgag gtccgccagc gccagcggac gcgcatcccg
     7081 cttctctcgc tcgctcctct tgcggaggtt ctcgaactcc gcgagcgacc gcaggtgccg
     7141 gtccttgagg ttttcgaggt cccgctcgac cgacttcaac ttctcttcgg tggccgcgat
     7201 tgcggaacgc tcttcctccg cgagcgccgc atcgagcgac tctcccgtgt cctcgaccag
     7261 gatccggtcg tcttcgatcg gatcgccgtc gtcgtcgtcg ccgcggtcaa tcatgggggt
     7321 tgccccgccg cgcccgcgaa tcacgactct cgagtcggtc ggtcagagcg cgcccgagca
     7381 gctcgacgac cgggatcacg cgcggatact cgcgacgcgc ggggccgatg actccgagca
     7441 ttccggtcag tgtctctccc gctccgaagg aggtcaagac ggccgacagc ctcgggtccg
     7501 acgccaggct cgcttccgaa ccgagcacga cgcacgtctc cttcgagtcg aggtaacggg
     7561 tcacgaggtc gaggagacgg gccttttcgt cgaacgcgcg gaagatccgc ttcagacttt
     7621 cgacgtcgga gaattcgggc ttgtcgagca gcttcatggc gccttcgacg tagacgcgct
     7681 cttccgtgtc gccgcggccg agcgcgagct ggccgagtgc gagcgagcgc gtgagcatcc
     7741 ggtcgtatgc ggccttctcc tccgtcatct ccgcgaccag ccggcgacgg acctcgtgca
     7801 gcgtcaagcc ggcgtattcg cgggtgaacc gttcgctggc ggtctcgagc tcgcgcgatc
     7861 cgtagtcgcc ggccgtctcg atcacccgcg acagaacgac gtcgggctcg ttcacctgga
     7921 cgaccagcac ctttcccgga gcaaccgcga gaaaacgcag gctcttgagc accgtgtgga
     7981 gggcgtcggg cgccaccacg aacccgacct cgcccgacag tctcgagagg agacgtgacg
     8041 cggcctggaa gatgccggtc atctccggac ccgccgaagc gaggtccgaa tcgacccgct
     8101 cacgcagccc ctcgttgact cgccggtggt gggcgagctc gtcgatgtag aggcggtagg
     8161 cgcggtcggt cggaaccctc ccggccgagg tgtgcggctg aaccaggaag cccgcgtcgg
     8221 tcaggtccgc catgatgttg cgaatggacg ccggcgagag gctgaagcgg tgcgacttga
     8281 agagcgtccg ggacgacacg ggtttccccg tgtccacgtg gacccggatg atttccttca
     8341 ggattgcgcg gctgcgcgtg tcgaggttgc tcatttctgt ctggcactcc ttcaggggga
     8401 gtgccaatca gcagggtacg ggttcgacgt tcaaagttca aggagcaagg ttgccggtgg
     8461 gcaaagcgct catcgggaat cgccttagac tcaccgggtg ccctccgacg tcggcaagac
     8521 cagcatcgcc ctcgtcgggc cgggccgcgc cgggcgggcg ttcgcacgct cctggaccgc
     8581 ggccgggggc tcgatcgtcc tcgtcgcccg cgatgcgggg gccgcccgag cgttcgcgcg
     8641 ggagctcccg cgcgcggagg tgctcgcgct cgacgatcga ctccggctcg cgtgcgacgt
     8701 gttgatcctg tcggtccccg acgacgcgat cgggacgctc gcgcgtcggc tctccccacg
     8761 cgtcgcctgc cgcttcgcgc ttcatttctc gggagcgctg ccgagcgcgg agctcgcgcc
     8821 cctgtcgtcc ggcggaacga aggtcgcttc gatacacccg cttcgcgcct tcagcggttc
     8881 gggagaagac aactggagcg gcgccttcgt cgcggtcgag ggggaagagg cggcagcgaa
     8941 gaccgcctca cggctctgtc gccacgtggg cgcgcgcccg caccctctct cggccgcgaa
     9001 caagcctctc taccatgctg ccgcgacgct cgccgccgga ggttcgggct cgctcctctc
     9061 cttcgccaca cgtctctgga cgcgagccgg cctcgccgag gaggacgggc agtcggctct
     9121 cgcgggcctc gctgcaggtt cggtcgaggc cgtgggacgg ctcccgttcg accgcgcgtt
     9181 gaccggcccc gtcgcgcggc gcgacgtcga aacgatccga ctccaccggg atgctctggc
     9241 gtgctctccc gaccttctgg agctttacgc tctgctcgca ggcgagactc tccggcgcac
     9301 tcccggccgg gggcgcgaag aggaaatcgc cggaattctc gcgagagagg gcgagcccgt
     9361 cggcgacccc gagcgcgaga gtgacaagcg ttcggacggc cccggccgaa agggctgacc
     9421 cgcagccgaa ttcgaccggc ccatcgaccg gctgcccctc cacggtgcta aaattcggga
     9481 gcgggagcac ttcccgcccg accacaaatg gtgtttttca actacgccac gatgcagatg
     9541 gcggccaaga tcgtttacta cggtccgggc ctgtgcggaa aaacgaccaa cctccaccac
     9601 atctacggcc gcacctcccc ggactcgcga ggcgagatgg gctcgctcga gacggagacg
     9661 gaccgcacac tcttcttcga cctgctcccc ttagacgtcg gcgtcatcag cggcttcaag
     9721 acgcgggtcc agctttacac ggtgcccggg caggtcttct acaacacgac gcgcaagctc
     9781 gttctgaagg gagtcgacgg gatcgtcttc gtcgccgact cccagcgcgc gatgcgcgag
     9841 gcgaacatcg aaagtctggc gaacctgcgc gccaacctcg cggagatcgg gatgaacctc
     9901 gaagaggtcc ctctcgtcct ccagtacaac aagcgagatc tcgcgaacat cctgtcggtc
     9961 gaggagctcg cggcggacct gaaccgggac cgggcgcacg agagctacga ggcgagcgcg
    10021 attctcgggc agggagtctt cgagacgttg aaggcgatct cccgcacgac gcttcgctcg
    10081 ctcaagaaac gcatggtcgg tgaggagcgg ccgtcgaagg cgtcgatccc gctcccgacg
    10141 cctctcccga aggccgaggt ggccgcacac ccgcccgcat ccccggatgt gactcggccg
    10201 gcgtacgtcg aagagaaggc gccggtcgtc gagagatccc gcgagaccgt cggttttcct
    10261 ccgcccgaac tcgcgccggc aaggccgact ccgggcgcca cagatatccc cgccgagatc
    10321 gagttcgatc cgaccgtggc cccgggggcc gcggcggccc gggccgacga catcgccgag
    10381 gtttcctttt cccaggccaa cgacatgccg gcggggtccg acctcggcgt caaacacgtt
    10441 cgcgtcggct cgaacgtcga catcctcgcg gagctcgaac gacttcggaa gaacgccacg
    10501 tccctgtccc cggccttgaa acccacgcga cgcgccgcct ccggaatctc cgtcgacgac
    10561 cttctggcga acagcctgaa tcaccgaaag gaattgagcc gggtctacga gctcgaagtc
    10621 ccgaggaagg agctcgccgc cggcgagcag atcaccgtga ctctttcggt caacgatcgc
    10681 aacaagcagc gcatcggcga ggagagaagt ttctcgatcc cgctcgaggc gcaggacctc
    10741 gagaagctcc tgctctcgct caaattccag attttcggca agtaggggcg tggagcgggc
    10801 ggcagaattc tgtcctcacc cggacacacc cccaaaaaag gttttgattt tggcttcctc
    10861 ccggcggtac cctttttcca gatgacccca aagacagtgc tcctccgggg gagagactct
    10921 gttctgttgt gtctgcttgt cgggctcttc ggctgcgcgg caaacccgcg tccgcccgcc
    10981 gtcgtccccc acccctcggc ggaatacgtc gcgatgacga aggcgtcgga gatcttcgcg
    11041 cgcggccgcg aggcggcgct cgctggcgac ttcgagtgcg cgagcgagta tttccggcag
    11101 tcgatcgaag ctctccggcc ggagaccggt ccggcgccca cggacccgga gctgctgtcg
    11161 ttctctttcc agctctacga gagcgctctc cgctacgagg ctctcgccgg accggcggag
    11221 gaggccggga cctccgacgg ccagatggcg tcggagttga acgagatcgc cgagccgcct
    11281 gcgaccgtcg aagccatggt ccacgcgaag gaagcggtcg actccgacac ggctgacctc
    11341 gcttacgaca tcccgatcac gatcaacgaa cccgtgctgc ggatcctcgc ggccttccag
    11401 aacgacgaac gggtccacag cgtcatcagc cgggggcttt cgcgctcggg ccgctacatg
    11461 cccatgatcc accggatctt cggcgaggaa gggatcccga aggacctcgc ccagatcgcc
    11521 ctgatcgaat cctcgtttct cccccacgcc
//
LOCUS       DASWAV010000678         2012 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U2665
            k141_39664, whole genome shotgun sequence.
ACCESSION   DASWAV010000678 DASWAV010000000
VERSION     DASWAV010000678.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960316
            Sequence Read Archive: SRR10260007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2012)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2012)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:47:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,929
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2012
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_39664"
                     /isolate="SMAG_U2665"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.36444 N 132.31889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..895
                     /gene="asnS"
                     /locus_tag="VF958_14300"
     CDS             <1..895
                     /gene="asnS"
                     /locus_tag="VF958_14300"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013809851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004816 - asparagine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006421 - asparaginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HEX9150323.1"
                     /translation="RSEVAQAIRDFFYERQYTLIDSPILTGSSVEGTSTLFETDYFGD
                     KAYLSQSGQLYLEPAAASFGKVYCFGPTFRAEKSKTRRHLTEFWMVEPEVAFLDFEGL
                     CELAEEFVVSLIARTLDRSAEDLKRLERDTSKLEAVTRPFPRITYREAVEKLAGAGFS
                     MKFGDDLGADEETALVADGERPLIVSRFPTAIKSFYMQPDPKDPEVVLGLDMLAPEGY
                     GEIIGGSQRIHDLALLERRIAEHKLPREAYEWYLDVRRYGTFPHSGFGMGLERFVAWM
                     CGIHHLREAIPYPRTLKRIYP"
     gene            933..1226
                     /locus_tag="VF958_14305"
     CDS             933..1226
                     /locus_tag="VF958_14305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012111516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="HEX9150324.1"
                     /translation="MKGIISSKGQITVPKSVRDRYAMGPGTEVEFELREDGALIRKRR
                     AQSHPIWDAIGSLRNSWRWPKGVPHTVDAYIDHVRGGSYEELTGKKPRRRSRK"
     gene            1223..1630
                     /locus_tag="VF958_14310"
     CDS             1223..1630
                     /locus_tag="VF958_14310"
                     /inference="COORDINATES: protein motif:HMM:NF013970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HEX9150325.1"
                     /translation="MITALDANILIDLGLEDSVRARQAARALDECGSAGKLVVCDVVL
                     AEFAGGLSDDIDPAAWVRDRGVEFDPIGEETAVRAGRMQARLESRTRRPWRRPIADFL
                     IGAHALVQADRLLTRDRGFYRDYFRGLRLIEPR"
     gene            1627..>2012
                     /locus_tag="VF958_14315"
     CDS             1627..>2012
                     /locus_tag="VF958_14315"
                     /inference="COORDINATES: protein motif:HMM:NF013112.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     RimO"
                     /protein_id="HEX9150326.1"
                     /translation="MNRKPPTVGVVSLGCPKNLVDSEVMLGHLQRAGHEIVPDSRARV
                     VLVNTCGFIDKAKEESVDAILEQVERKRRGEVDRIVVAGCMVQKYGRELADAIPEVDA
                     FLGLDELERAPAAAGHPAFPRFTDKPL"
BASE COUNT          361 a          640 c          661 g          350 t
ORIGIN      
        1 ccgctccgag gtggcgcagg ctatccgcga ctttttctac gagcgtcagt acacgctcat
       61 cgattcaccg atcctgacgg gatcgtcggt cgaggggacc tcgacgctct tcgagaccga
      121 ctacttcggc gacaaggcgt acctctcgca gtcggggcag ctctatctcg agcccgcggc
      181 cgcctcgttc ggaaaggttt actgcttcgg tccgacgttc cgcgccgaaa agtcgaaaac
      241 gcgccgccac ctgaccgagt tctggatggt ggagccggag gtcgcgttcc tggacttcga
      301 gggtctctgc gagctcgccg aggagttcgt cgtgtctctg atcgcgcgga cactcgaccg
      361 cagcgccgaa gatctcaaac gcctcgaacg cgacacgtcg aagctcgagg ccgtgacccg
      421 accgtttccg cggatcacgt accgcgaggc ggtggaaaag ctcgctggcg caggcttctc
      481 gatgaagttc ggcgacgatc tcggcgcgga cgaagagacc gcgctcgtcg ccgacggcga
      541 gcggcctctc atcgtgtccc gcttcccgac cgccatcaag tcgttttaca tgcagccgga
      601 cccgaaagac cccgaggtgg ttctcgggct cgacatgctg gcgcccgagg gatatggcga
      661 gatcatcggc ggctcgcagc gcatccacga cctcgcgctc ctcgaacgcc ggatcgcgga
      721 gcacaagctt ccgcgcgagg cgtacgagtg gtacctcgac gtccggaggt acggaacgtt
      781 cccgcactcg ggcttcggga tggggctcga acgcttcgtc gcgtggatgt gcggaattca
      841 ccatctccgc gaggcgatcc cgtacccgcg aacgttgaag aggatctatc cctagtcttt
      901 actccggcgc tttacttctt tactatgcag gtatgaaggg cattatctcg tcgaaaggac
      961 agatcaccgt cccgaagagc gtgcgagacc gatacgcgat ggggcccggg accgaggtgg
     1021 aattcgagct ccgcgaagat ggcgctctca tccgcaagcg gagggcacag agccacccga
     1081 tctgggacgc gatcggctca ctcaggaact cctggcgctg gccgaagggc gtcccccaca
     1141 cggtggacgc ctatatcgat cacgtgcgtg gagggtccta cgaagagctc accggaaaaa
     1201 aaccccgccg gagaagtcgc aagtgatcac cgccctcgac gcgaacatcc tgatcgacct
     1261 cgggctcgag gattcagtgc gtgcgagaca ggctgcgcgg gcactcgacg agtgtggctc
     1321 ggcaggaaag ctcgttgtct gcgacgtggt gctcgcggag ttcgcgggcg gactctcgga
     1381 cgatatcgat ccggcggcct gggtccgtga tcggggcgtc gagttcgacc cgatcggaga
     1441 ggagactgcc gtccgcgcgg gtcggatgca ggcgcgactc gagagtcgta cccggcgtcc
     1501 atggcgacgc ccgattgccg acttcctcat cggtgcccac gcgctcgttc aggcggatcg
     1561 tctcctcacc cgcgatcgag gcttctaccg cgattacttt cgcgggctcc gcctgatcga
     1621 gccgagatga accggaagcc cccgaccgtc ggtgtcgtgt cgctcggatg cccgaagaac
     1681 ctcgtcgaca gcgaggtcat gctcggacac ctccagcggg ccggccacga gatcgtgccc
     1741 gactcgaggg cgcgggtcgt gctcgtcaac acgtgcgggt tcatcgacaa ggcgaaagaa
     1801 gagtcggtcg acgcgatcct cgagcaggtc gagcgaaagc gccgcggaga ggtcgatcgc
     1861 atcgtggtcg cgggatgcat ggtgcagaag tacggccggg agctggctga cgcaatcccg
     1921 gaagtggacg cgtttctcgg cctggacgag ctcgagcgag cgcctgcggc ggcggggcat
     1981 ccggctttcc cccggttcac cgacaagcct ct
//
LOCUS       DASWAV010000679         5762 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U2665
            k141_439277, whole genome shotgun sequence.
ACCESSION   DASWAV010000679 DASWAV010000000
VERSION     DASWAV010000679.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960316
            Sequence Read Archive: SRR10260007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 5762)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5762)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:47:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,929
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5762
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_439277"
                     /isolate="SMAG_U2665"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.36444 N 132.31889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(60..3677)
                     /gene="nifJ"
                     /locus_tag="VF958_14320"
     CDS             complement(60..3677)
                     /gene="nifJ"
                     /locus_tag="VF958_14320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015182979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HEX9150327.1"
                     /translation="MNRDKNWITVDGNEAVASVAHRLSEVIAIYPITPSSAMGELADE
                     WSAAGRRNVWDAVPQVVEMQSEGGAAGAVHGALQAGALATTFTSSQGLLLMIPNMYKI
                     AGELTPFAMHVAARTLATHALSIFGDHSDVMACRQTGFALLASGSVQEAQDMAAIAHA
                     ATLECRIPFLHFFDGFRTSHEVAKIERLSDEDLKALVSDQRIAEHRARALTPDRPVIR
                     GTAQNPDVFFQAREACNPFFAACPHVVRETMERFAAITGRRYGLFDYVGDPEAEHVVV
                     VMGSGAEVVHETVEWMAARGVRVGVLKVRLFRPFSGPDFIAALPPTAKKISVLDRTKE
                     PGAIGEPLYQDVVTALAEARDSGLRVERFGATVVGGRYGLSSKEFHPGMVKAVFDALR
                     SEKPKNHFTVGIQDDISGTSLPFDGDFDIEPSSVVRGVFFGLGADGTVSANKNSIKII
                     GETTEGAAQGYFVYDSKKSGAITISHLRFGPAPIRSSYLVRKAQFVACHQVGLLDRYD
                     VLGYAAEGANVLLNCPGGAESAWNRLPQEAQQQILEKRLAVHAIDADRVAREMGLTGR
                     INTIMQTCFFAISGILPREDAIRRIKQSIAKSYGKKGADVLAKNLAAVDRALENLARV
                     EVPDSVTSAKVIPFPVPDDAPDFVRRVTATIIAGHGDALPVSAFPPDGTWPSGTSRYE
                     KRNIADEIPVWDPSLCIQCNKCVLVCPHAAIRAKVYEPAFLEGAPAGFLADTFRGLDF
                     KDWKYTIQVAPEDCTGCSLCVVICPAHDKKDKSRKSLRMEAQPPRLEAERNSFEFFEE
                     LPEVDRTRLRVVDVKGSQFFQPLFEYSGACSGCGETPYIKLLTQLFGDRTLIANATGC
                     SSIYGGNLPTTPYTKNREGRGPAWANSLFEDNAEFGLGFRLAADGREDRVRNHVARFA
                     DLLAPGLVDEILRSPQGTEAQIAAQRDRVEKLRAELARLTGSDARQLEIDADALVKKS
                     VWIVGGDGWAYDIGFGGLDHVLASGRNVNVLVLDSEVYSNTGGQQSKATPLGAAAKFA
                     AAGKETGKKDLGLMAMTYGNVYVARIAFGAKDHQTVKALLEAESYPGPSLVIAYAHCI
                     AHGYDMADGTNQQKLAVDSGTWPLYRFDPRRIAEGQPPLQLDSPPAKIPIEQYMHNEN
                     RFRMLERADAGRWRQLVDAAQKEVERRTGLYQKLAALSPAGWGAAESKKPEPEIAEVT
                     T"
     gene            3958..>5762
                     /gene="hypF"
                     /locus_tag="VF958_14325"
     CDS             3958..>5762
                     /gene="hypF"
                     /locus_tag="VF958_14325"
                     /EC_number="6.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011419535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003998 - acylphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyltransferase HypF"
                     /protein_id="HEX9150328.1"
                     /translation="MGFRPWIWRLARETGVNGRVSNDSRGVTIEAFGSQKALETFLDG
                     IRTSPPPAAEVRDLTWNAIPHEDAREFVIVASRVSEGLEVSIPPDLATCGDCLEEIFD
                     PSNRRYRYPFTNCTNCGPRFTIAREVPYDRPATTMAVFPMCSRCRAEYEDPADRRFHA
                     QPNACAACGPMLRALSADGEPIDWMDPLRGTARALCAGLIVAIKGLGGFHLACDATSS
                     DAVTRLRRRKRREERPFAVMVRDLDAADALAQVSPAERALLASTERPIVLLARREGSA
                     IAPEVAPDTPLVGLMLPYTPLHHLLLAETGRPLVMTSGNLSEEPIACGNAEAVARLSR
                     IADGFLVHDRKIESRCDDSVVRVIAGRPTVLRRSRGWVPRSIPVAVPFPEPVLACGGH
                     LKNTFCIGLDGAAYLGPHIGDLENLETLAAYEEAINRLERFLRVRPEVIAHDAHPGYV
                     STVYARETRTGRKVAVQHHHAHIASAMAEHRLAGPVLGVAYDGTGYGTDGTAWGGEVM
                     RADGATYERFGTFRPIALAGGDTAIREVWRIALAMLSDAYPEGPPLSLLPLFGGIDPH
                     HLDVVRRMLAGGVSCPRARGVGRYFDAFGALFLERP"
BASE COUNT          896 a         1906 c         1985 g          975 t
ORIGIN      
        1 cttgccttcg agaggaacga gacggccgac cggcagctcc gtgaagtcct cgacgcggat
       61 catgtcgtca cctccgcgat ttcaggttcc ggtttcttcg attccgccgc tccccagccg
      121 gcgggggaga gtgcggcgag cttctggtag agacccgtgc ggcgctcgac ttccttctgc
      181 gccgcgtcga cgagctgccg ccaccggccg gcatccgcgc gttccagcat ccggaagcga
      241 ttctcgttgt gcatgtactg ctcgatcggg atcttggcgg gcggcgagtc gagctggagc
      301 ggcggctggc cttccgcgat ccggcgcgga tcgaagcggt agagcggcca cgttccggag
      361 tcgacggcga gcttctgctg gttcgtgccg tccgccatgt cgtagccgtg cgcgatgcag
      421 tgagcgtacg cgatgacgag cgacggtccc ggataggact ccgcctcgag gagcgctttc
      481 acggtctggt ggtccttggc gccgaaggcg attcgggcaa cgtagacgtt gccgtaggtc
      541 atcgccatca agccgagatc cttctttccg gtctccttcc cggcggccgc gaacttcgcg
      601 gcggcgccca agggcgtggc cttcgactgc tgccccccgg tgttcgagta gacctcgctg
      661 tcgagcacga ggacgttgac gttgcggccg ctcgccagca cgtggtcgag ccccccgaac
      721 ccgatgtcgt acgcccatcc gtcaccgccg acgatccaga cgctcttctt tacgagcgcg
      781 tcggcgtcga tttcgagctg tcgtgcgtcg cttcccgtca ggcgcgcgag ctcggcgcgc
      841 agcttctcga cgcgatcgcg ctgagcggcg atctgcgctt ccgtcccctg gggcgacctg
      901 aggatttcgt ccacgagacc cggggcgagc aggtcggcga agcgggcgac gtgattccgg
      961 actctgtctt cgcggccgtc ggcggcgaga cggaacccga gcccgaactc cgcgttgtcc
     1021 tcgaagagcg agttcgccca ggcgggaccg cgcccttcgc ggttcttcgt gtacggcgtc
     1081 gtcgggagat tgcctccgta gatcgagctg cagcccgtcg cgttcgcgat cagcgtccgg
     1141 tcaccgaaaa gctgggtcag gagcttgatg taaggcgtct cgccgcaacc cgagcacgcg
     1201 ccggagtact cgaacagcgg ctggaagaac tgcgagccct tgacgtcgac gacccggaga
     1261 cgggttcggt cgacctccgg cagctcctcg aagaactcga agctgttgcg ctcggcttcg
     1321 agccgcggag gctgcgcctc catgcggagg gactttcgac tcttgtcctt cttgtcgtgg
     1381 gcgggacaga tgacgacgca cagagagcag cccgtgcagt cttcgggcgc gacctggatc
     1441 gtgtacttcc agtccttgaa gtcgagcccg cggaacgtgt cggcaaggaa cccggccggc
     1501 gccccttcga gaaaggccgg ctcgtacacc ttggcgcgga ttgcggcgtg ggggcagacg
     1561 agaacgcact tgttgcactg gatgcagagc gacgggtccc acaccgggat ctcgtcggcg
     1621 atgtttcgct tttcgtaccg gctcgtgccg gacggccacg tcccgtccgg cgggaaggcg
     1681 gagaccggaa gggcgtcacc gtgcccggcg atgatggtcg cggtgacccg gcggacgaag
     1741 tcgggagcat cgtcggggac cggaaaggga atcaccttcg ccgacgtgac ggaatccggg
     1801 acctcaacgc gggcgagatt ctcgagtgcg cggtccaccg ccgcgaggtt cttcgccagc
     1861 acgtcggcgc ccttctttcc gtagctcttc gcgatcgact gcttgatgcg ccggatcgca
     1921 tcctcgcggg gcaggattcc ggagatcgcg aagaagcacg tctgcatgat cgtgttgatg
     1981 cggccggtga gccccatttc acgcgccaca cggtcggcgt cgattgcgtg aaccgccagg
     2041 cgcttctcga ggatctgctg ctgggcctcc tgcggcagcc tgttccatgc cgattcggca
     2101 ccgcccgggc agttcaacag gacgttggcg ccctcggcgg catagccgag gacgtcgtag
     2161 cggtcgagca gtccgacctg gtggcacgcg acgaactgcg cctttctcac gagatacgag
     2221 gagcggatgg gcgcgggacc gaagcgcagg tgcgagatcg tgatcgctcc ggacttcttg
     2281 gagtcgtaga cgaagtagcc ctgcgcagcg ccttccgtgg tttccccgat gatcttgatc
     2341 gagttcttgt tggcgctgac ggtgccgtcc gcaccgagcc cgaagaagac gccccgcacg
     2401 accgacgacg gctcgatgtc gaaatcccca tcgaacggaa gcgaggtgcc cgaaatgtca
     2461 tcctgaatcc cgacggtgaa gtggttcttc ggcttctctg accggagcgc gtcgaagacc
     2521 gccttgacca tgcccggatg gaactccttc gaggacaggc cgtaccggcc gccgacaacg
     2581 gtcgcgccga atcgctcgac gcggaggccg gagtcgcgcg cctcggcgag cgccgtgacg
     2641 acgtcctggt agagcggttc gccgatcgcg cccggttcct tcgtccgatc gaggaccgag
     2701 atcttcttcg cggtcggagg cagggcggcg atgaagtccg ggcccgaaaa cgggcggaag
     2761 aggcggacct tgagcacccc gactcgcacg ccgcgcgcag ccatccattc gaccgtctcg
     2821 tgaacgacct cggcgcccga gcccatcacg acgacgacgt gctcggcctc gggatcgccg
     2881 acgtagtcga agagaccgta gcggcgtccg gtgatcgccg caaagcgctc catcgtttcc
     2941 cgcacgacgt gcgggcaggc cgcaaagaac ggattgcacg cttcgcgcgc ctggaagaaa
     3001 acgtcgggat tctgagccgt cccgcggatg acgggccggt cgggcgtcaa cgcgcgggcg
     3061 cggtgttcgg cgatccgctg atcggacacg agcgctttca ggtcctcgtc ggaaagccgc
     3121 tcgattttcg cgacctcgtg ggaggtccgg aagccgtcga agaagtggag gaaggggatg
     3181 cggcactcga gcgtcgcggc atgcgcgatt gccgccatgt cctgggcctc ctggaccgac
     3241 cccgacgcga ggagcgcgaa acccgtctgg cggcaggcca tcacgtcgga gtggtcgccg
     3301 aagatggaga gtgcgtgggt ggcgagcgtg cgcgccgcca cgtgcatggc gaacggcgtc
     3361 agctcccccg cgatcttgta catgttcggg atcatcagca ggaggccctg cgaggacgtg
     3421 aatgtcgtgg cgagcgcgcc cgcctggagt gccccgtgga ccgcgccggc tgcgccgcct
     3481 tccgactgca tctcgacgac ctggggaacg gcgtcccaga cgttgcgccg gccggcggcc
     3541 gaccattcgt cggcgagctc gcccatcgcc gaggagggcg ttatggggta gatcgcgatg
     3601 acttccgaga ggcgatgcgc gaccgaagcg acggcctcgt tgccgtcgac ggtgatccag
     3661 tttttgtccc tgttcatggg gagaacttag gcggcggcgt cacctttccc atgatgcgga
     3721 tgcatcagcg cgccgaacgc ggtcccggga ggcgcggcag cagcactcga tcgggcccgc
     3781 gcccgccgtg gcgctgatgc atttgcacca cggcgccggc ggtctcacgt cctagtttga
     3841 aggccgaacg agaagggaaa tgaagagcca gcgcgttccg agcttcgaga gacggatggg
     3901 aggcatgccc tgacggccga aggtcggcac atcgaagtgc gaggaacggt ccagggcgtg
     3961 ggattccgcc cgtggatctg gaggctcgcg cgcgagaccg gcgtcaacgg gcgggtctcg
     4021 aacgactcgc gcggcgtcac gatcgaggcc ttcggcagtc aaaaggctct cgagacgttc
     4081 ctggacggca tccgcacttc gccgccgccg gcggcggagg tccgcgacct gacctggaac
     4141 gccatcccgc acgaggacgc ccgcgagttc gtgatcgtcg cgagccgcgt gagcgaaggg
     4201 ctcgaagtct cgatcccgcc ggatctcgcg acgtgcggcg attgcctcga ggagatcttc
     4261 gatccgtcca accggcgtta tcggtacccg ttcacaaact gcaccaactg cggaccgaga
     4321 ttcacgattg cgcgggaggt cccctacgac cggccggcga cgacgatggc cgtcttcccg
     4381 atgtgctcgc gttgtcgcgc ggaatacgag gatcccgccg accgccgctt ccacgcgcaa
     4441 cccaacgcgt gcgcggcgtg cggaccgatg ctccgagctc tctcggccga cggcgagccg
     4501 atcgactgga tggatccgct ccgcgggacc gcgcgggctc tctgcgccgg gttgatcgtc
     4561 gccatcaagg gactcggtgg gttccatctg gcctgcgacg cgacgtcgtc ggacgccgtc
     4621 acgcgcctgc gccggcgcaa gcggcgcgag gagcgaccgt tcgcggtcat ggtgcgagac
     4681 ctcgacgccg cggacgccct cgcgcaggtg tcgccggcgg agcgggcgct gctcgcctcc
     4741 acggagagac cgatcgtcct cctcgcgagg agagaaggct ccgcaatcgc tcccgaggtc
     4801 gcgccggata cgcctctggt cgggttgatg cttccttaca ctcctcttca ccatctcctg
     4861 ctcgccgaaa ccgggcggcc gctcgtcatg acctccggga acctctcgga agagccgatc
     4921 gcctgcggga acgcggaagc ggtcgcgcgt ctctcccgca tcgccgacgg gtttctcgtg
     4981 cacgaccgga agatcgagtc ccgttgcgac gactcggtcg ttcgagtgat cgcgggccgc
     5041 ccgacggtgc tgcgccgctc gcggggctgg gtgccgcgtt cgatccccgt cgcggttccc
     5101 tttcccgaac cggtcctcgc ctgcggcggt catctgaaga acaccttctg catcgggctc
     5161 gacggcgcgg cgtacctggg accacacatc ggagacctcg agaacctcga gactctcgcc
     5221 gcgtacgagg aagcgatcaa tcggctcgag cggttcctgc gggttcgacc cgaggtgatc
     5281 gcccacgacg cgcaccccgg gtacgtctcg acggtctatg cgcgggagac gcgcaccggc
     5341 cggaaggtcg cggtgcagca tcaccatgcc cacattgcga gcgcgatggc cgagcaccgc
     5401 ctcgccggac ccgttctggg agtggcgtac gacggcacgg gatatggcac cgacggcacc
     5461 gcgtggggtg gcgaggtcat gcgcgcggac ggggcgactt acgagcgctt cgggacgttc
     5521 cgtccgatcg cgctcgcggg aggcgacacc gccatccgcg aggtctggcg gatcgcgctg
     5581 gcgatgctct cagacgctta tccggaaggg ccgccgctgt cgctccttcc tctcttcggc
     5641 ggaatcgatc cgcaccactt agacgtcgtc cgccggatgc tcgcgggcgg cgtgtcctgt
     5701 ccgcgggccc gtggcgtggg acgttacttc gacgcgttcg gcgcgctctt cctggaacgg
     5761 cc
//
LOCUS       DASWAV010000680         2046 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U2665
            k141_799775, whole genome shotgun sequence.
ACCESSION   DASWAV010000680 DASWAV010000000
VERSION     DASWAV010000680.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960316
            Sequence Read Archive: SRR10260007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2046)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2046)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:47:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,929
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2046
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_799775"
                     /isolate="SMAG_U2665"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.36444 N 132.31889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..369
                     /locus_tag="VF958_14330"
     CDS             <1..369
                     /locus_tag="VF958_14330"
                     /inference="COORDINATES: protein motif:HMM:NF014439.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HEX9150329.1"
                     /translation="QIDDAFGPELFVTSVHGRNVLLYPLAIWRALEEKLAALPAVHRA
                     KNKFLERVNYYGQDASLDGQGRVLIPQILRDAVKLPADVVVTGNIDHLVVSDRGALEG
                     RLTTEDFTDEDYDELSKALS"
     gene            376..1365
                     /gene="rsmH"
                     /locus_tag="VF958_14335"
     CDS             376..1365
                     /gene="rsmH"
                     /locus_tag="VF958_14335"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015751188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HEX9150330.1"
                     /translation="MGKTENGKRKERSRTNDVDKPPHQPVLLREVVELLAPERGGFFV
                     DATVGAGGHARALLEAGPDLRLLGLDRDPDALALARGRLDEFGDRVRLVEANFADLGV
                     VLAGSPPPDGILADLGVSSMQLEEPSRGFSFRREGPLDMRMSRSGRSAADVIAHASVE
                     ELSRIFLQYGEEWMAIKIARAILEERSRGPITTTRQLSRIVARVKGDREKIDPATRVF
                     QALRIEVNEELQSLSRFLVAAVEKLNAEGRVAVIAYHSLEDRMVKETFRRESGVCFCP
                     PRLPICVCGARRVLKVLTRRPIRPSDAEVRRNPRSRSARLRAAEKLSGERAAI"
     gene            1362..1718
                     /locus_tag="VF958_14340"
     CDS             1362..1718
                     /locus_tag="VF958_14340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9150331.1"
                     /translation="MSSGVYVQSKRVENLHLTRERDRRRGRELATYLLAGLPIAFALL
                     AWAALHVETVRVGYARGALDRKFAELTEENRRLRAELGRASAPDRVQAVAVRKGLAPP
                     RPGQVQYVEPREGSVR"
     gene            1715..>2046
                     /locus_tag="VF958_14345"
     CDS             1715..>2046
                     /locus_tag="VF958_14345"
                     /inference="COORDINATES: protein motif:HMM:NF015662.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9150332.1"
                     /translation="MNFVRLARVRFLALFLMLWASVVVARLAELQLAQGSRYRARAQR
                     QQERRIEVSPLRGSIFDREGRPLAVSVEVSSVYAIPDDVKDPAETARTLAGHLDVPEA
                     ALLARLTQ"
BASE COUNT          332 a          696 c          685 g          333 t
ORIGIN      
        1 cagatcgacg acgccttcgg accggaactc ttcgtgactt cggtgcacgg aaggaacgtc
       61 ctcctgtacc cgctcgcgat ctggcgcgcc ctcgaggaga agctcgcggc gctgcccgcc
      121 gtgcaccgcg cgaagaacaa gttcctcgaa cgagtcaact actacgggca ggacgcgagc
      181 ctcgacggcc agggtcgcgt cctgattccg cagattctcc gcgacgccgt aaagctcccc
      241 gcggacgtcg tcgtgaccgg gaacatcgat cacctcgtcg tttcggatcg cggcgcgctc
      301 gaaggacggc tgaccacgga ggacttcacc gacgaggact acgacgagct gtcgaaggcg
      361 ctgtcgtgag ccgcgatagg gaaaacggaa aacgggaaac gaaaagagag aagtaggacg
      421 aacgacgttg acaagccgcc gcatcagccg gtcctgcttc gagaggtggt cgagctgctc
      481 gcgccggagc gcggcgggtt cttcgtggac gcgacggtcg gcgcgggagg ccatgcccgc
      541 gctcttctcg aggcagggcc cgacctccgg ctcctcggcc tcgaccgcga tcccgatgcg
      601 ctcgctctcg cgcgcgggcg gctcgacgag ttcggcgacc gggtgcgcct cgtcgaggcg
      661 aatttcgccg atctcggcgt ggtgctcgca ggctccccgc ctcccgacgg gatcctggcg
      721 gatctcggcg tgtcctcgat gcagctcgag gagccttcgc gcgggttctc gttccgccgg
      781 gaaggccctc tcgacatgcg gatgtcccgc tcgggtcgaa gtgccgccga cgtcatcgca
      841 cacgcttcgg tcgaagagct gagtcgaatt tttttgcagt acggggagga atggatggcg
      901 ataaaaatcg cgcgggcaat cctggaggag cgctcccggg gaccgatcac gaccacccgg
      961 cagctctcac gcatcgtggc gagggtcaag ggggacaggg aaaagatcga cccggcgaca
     1021 cgagtgttcc aggcactgcg catcgaggtc aacgaggagt tgcagtcgct ctcccgtttc
     1081 ctcgtggcgg ccgtcgaaaa gctgaacgcg gaagggcggg tcgccgtcat cgcctaccac
     1141 tctctcgagg accgcatggt gaaggagacc tttcggcgcg agtcgggagt ctgcttctgc
     1201 ccgccgaggc ttccaatctg cgtctgcggg gcgcgccgcg tactcaaggt cctgacgcgc
     1261 cgtccaatcc gtccttcgga cgccgaagtc cgccgcaacc cccggtcgcg cagcgcccgt
     1321 ctccgcgcgg ccgagaaact ttccggagag agagccgcca tttgagcagc ggggtctacg
     1381 tccagtcgaa gcgcgtcgag aacctgcatc tgacgcgcga gcgagaccgc cgccgcggcc
     1441 gcgagctcgc gacttacctt ctcgccggac tgccgatcgc gttcgcgctc ctggcctggg
     1501 cggctctcca cgtcgagacc gtgcgtgtgg gttatgcccg cggcgcgctc gaccgcaagt
     1561 tcgccgagct gactgaagaa aatcgccgcc tccgagccga gctcgggcgc gcctccgcgc
     1621 ccgatcgcgt tcaggccgtc gcggtgcgta aaggcctggc gcccccgcgg ccgggacagg
     1681 tccagtacgt cgagccgcga gaagggtccg tccgatgaac ttcgtgcgac tcgcccgcgt
     1741 ccggtttctc gcgctcttcc tcatgctgtg ggcttcggtc gtcgtcgcac ggctcgccga
     1801 gctgcagctt gcccagggca gtcgttatcg cgcccgagcc cagcgccagc aggagcggcg
     1861 aatcgaggtg tcgcccctgc gtggctcgat cttcgatcgc gaaggccgcc cgctcgcggt
     1921 gtccgtcgag gtctcctcgg tctacgcgat ccccgacgac gtcaaagacc cggcggaaac
     1981 agcgcgcact ctcgcggggc acctcgacgt tccggaggcg gcgcttctcg cgcgcctgac
     2041 acagaa
//
LOCUS       DASWAV010000681         3992 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U2665
            k141_759543, whole genome shotgun sequence.
ACCESSION   DASWAV010000681 DASWAV010000000
VERSION     DASWAV010000681.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960316
            Sequence Read Archive: SRR10260007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 3992)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3992)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:47:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,929
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3992
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_759543"
                     /isolate="SMAG_U2665"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.36444 N 132.31889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..418)
                     /locus_tag="VF958_14350"
     CDS             complement(<1..418)
                     /locus_tag="VF958_14350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9150333.1"
                     /translation="MPPSRPLVAVLAPEPIRPRMAGMGIRALELARAIGEELDVRLLV
                     ANDPVEAREVADDVPIVHAPPAADLVRSAAGVGAAIVSGHAANYWFHQMPDVAVAVDL
                     YDPFPVENLHYARELGAETARHDLATLHLALARGDFF"
     gene            complement(426..2294)
                     /locus_tag="VF958_14355"
     CDS             complement(426..2294)
                     /locus_tag="VF958_14355"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HEX9150334.1"
                     /translation="MAPVREDELIPYAPSDLTAGRVLVLAAHPDDEILGAGGLLALCA
                     ERGSAVRIWIATDGTAQERAEAEGAASYGELRREESRRAARVLGVDPPFFGGLSDRGL
                     DPEDEELRRAVAALIAEFEPDLVLCPSPAEIHPDHRALADVLFRTVAESRPADPDHDR
                     HRFLRIAFYEISQPFLPNALVDVSSVASKKEGALAAYDSQQSVRDYAGAVRGLNAYRR
                     LTLPGSGPVEAFCVLSWAEASSRSLEEFRRAIGPAIVRDGSRGTAPLAVVVRTRNRPA
                     LLAQALESLRSQTVRPARVVVVNDGGASVCSVVEPFRDAFAVSLEELPERRGRSAAAN
                     LGVESADQDLVAFLDDDDVVFPDHVDRLVSAHRAGPEPVVYSDAVTVVYRREGEEWRA
                     VSRTLQYSLDFDRDYLLLANYIPLHTLLLPRALYRKVGGFDAGMEFSEDWDFLIRASF
                     ETSFRHVRAVTCEYRVFQGDDGDPAHAASGSASFQQARAEIYGRYASRRTEEGFARAF
                     DRMRSQIAFWYERDTVSQGELKYQRESHRRLAQSFERALEERLTSAERRNSELAAENE
                     LVHDRLAELFAKNEELDLQLLGVYGENERLNGILNQIYGSRTWKLHLLLDRLRGRR"
     gene            complement(2330..3277)
                     /locus_tag="VF958_14360"
     CDS             complement(2330..3277)
                     /locus_tag="VF958_14360"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024917.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX9150335.1"
                     /translation="MSGFVIREATAGDASGIRRLFARVFGKEMSEAEWTWKFEQNPDG
                     WFGIVALVGEEIVGNYAGWGTRLLLDGRPALSYSVGDVATDSAVRGMGGRRGIYRAMT
                     EAFYEAVGARGVPFCFGFPNPRALEVSHRIVGSRTLFPVREVLVPCDAFPAPPLDAVA
                     GDCATESFDPLWRRAATFLTHAAVRDRGRANWRFHARPARWYRMLSRELRGESVGWAV
                     LSVTGDTALVADFLGREADGADLPALFAAAASEARRLGASKLVFWETPGGPAADLIRR
                     LPGDRRAAGFPIIVRTFDDEAADRFAAKAHLVPSLYDLV"
     gene            complement(3274..>3992)
                     /locus_tag="VF958_14365"
     CDS             complement(3274..>3992)
                     /locus_tag="VF958_14365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9150336.1"
                     /translation="AAAPLSFWDDADRLRLPPGWQPGFRSDLAQLSAGRRPARDDRRF
                     ASHARETLRLWQSGGEARQLSGTVLAARRETFDRVGRFDERFPFEYEETEWEDRVRAR
                     GLALRFVPSARVRHRWGSSAAGAGETAARRARSRQLYWRRRYGRLGGAMLERTGRRRS
                     EVSFPRTLDPRLAAREGAWAAVSTNASLLPFAGAPLDRDFVLPSEVADRLPPVPLFLR
                     SFRASDGHPIETFLWEAERE"
BASE COUNT          661 a         1397 c         1394 g          540 t
ORIGIN      
        1 ggaagaagtc gccgcgcgcg agcgcgaggt gaagcgtcgc gaggtcgtgc cgcgccgtct
       61 cggctccgag ctcgcgggcg tagtgcaggt tctcgacggg aaacgggtcg tagaggtcca
      121 cggccacggc cacgtcgggc atctgatgaa accagtagtt cgcggcgtgg cccgagacga
      181 tcgccgctcc gaccccggcg gcactgcgca cgagatctgc cgccggagga gcgtgaacga
      241 tcggaacgtc gtccgcgacc tccctcgctt ccaccggatc gttcgccacg agaagccgga
      301 catccagctc ctctccgatc gcgcgggcga gctcgagagc gcggattccc atccccgcca
      361 tgcgcggacg gatcggttcg ggagcgagga ccgcgacgag cggtcgcgac ggcggcatcg
      421 ttttcctacc gccggccgcg caggcggtcg aggagaaggt gcagcttcca ggtccgcgac
      481 ccgtagatct ggttcaggat gccgttcagc cgctcgttct ctccgtagac gcccaggagc
      541 tgaaggtcga gctcctcgtt tttcgcgaag agctcggcga ggcgatcgtg tacgagctcg
      601 ttctcggcgg cgagttccga gttgcgtcgc tcggcgctcg tcagcctctc ctccagcgcc
      661 cgctcgaaag attgcgccag ccgccggtga ctctcgcgct gatacttgag ctccccctgc
      721 gagaccgtgt cgcgctcgta ccagaacgcg atctgggaac gcatccggtc gaacgctcgg
      781 gcgaaaccct cttcggtccg gcgcgaagcg taacggccgt agatctcggc tcgcgcctgc
      841 tgaaacgacg cggaaccgga agcggcgtgc gccgggtcgc cgtcatcgcc ctggaagacg
      901 cggtattcac acgtgaccgc gcggacgtgg cggaaggacg tttcgaaaga agcacggatc
      961 aggaagtccc agtcctccga gaactccatc cccgcgtcga agccgccgac ctttcgatac
     1021 agcgcgcggg gaagaaggag ggtgtggagg gggatgtaat tcgcgagcag gagatagtcc
     1081 cggtcgaagt cgagcgagta ctggagggtc cgggagacgg ccctccattc ttccccttcg
     1141 cggcggtaga caaccgtcac cgcgtcggag tagacgaccg gctccggtcc ggcccggtgc
     1201 gccgaaacga gccgatcgac gtgatccgga aacacgacgt cgtcgtcgtc gaggaatgcg
     1261 acgaggtcct ggtccgcgga ttcgacaccg aggtttgccg ctgccgagcg gccgcgccgc
     1321 tccgggagct cctcgagaga gaccgcgaag gcgtcgcgga acggctcgac gaccgagcag
     1381 acggaagcgc cgccgtcgtt gacgacgacg acccgcgccg gccgaacggt ctgagaccga
     1441 aggctctcga gagcctgggc gaggagcgcg ggtcggttgc gcgtcctcac gacgacggca
     1501 aggggggcag tgccccggct gccgtcccgg acgattgccg gaccgatcgc gcgtcggaat
     1561 tcttcgagtg agcggctcga cgcctccgcc cacgagagca cgcagaacgc ttcgaccggc
     1621 ccgctcccgg gcagcgtcag tcggcggtag gcgttcagtc cgcggacagc ccccgcatag
     1681 tcgcggaccg actgttgcga gtcgtaggcg gcgagcgccc cctccttctt cgaggcgacc
     1741 gaggagacgt ccacgagcgc gttcgggaga aatggctgcg agatctcgta gaacgcgatc
     1801 cggagaaaac ggtggcggtc gtggtccgga tccgccgggc gcgactcggc gacggtccga
     1861 aagagcacgt ccgcgagagc gcgatggtcc gggtggatct ccgcgggaga cgggcagagg
     1921 acgagatcgg gctcgaactc cgcgatcagg gcggcgacgg cgcggcggag ctcttcgtcc
     1981 tcgggatcga gcccgcggtc ggagaggccg ccgaagaacg gtggatcgac tccgagaacc
     2041 cgcgctgccc gccgggactc ttcccgccgg agctcgccgt aggaggccgc gccctcggcc
     2101 tccgctcttt cctgcgccgt tccgtcagtg gcgatccaga tccggacggc gctcccccgc
     2161 tcggcgcaga gtgcgaggag acctccagcc ccgaggatct cgtcgtcggg atgggctgcc
     2221 agaacgagga ccctcccggc ggtcaagtcg ctcggcgcgt aggggatgag ctcgtcttct
     2281 ctcacgggcg ccatctgttt acgttcgagg gagtttaacc gttcgagtgt cagacgaggt
     2341 cgtacagcga cgggacgaga tgcgccttcg cggcgaatcg gtccgcggcc tcgtcgtcga
     2401 acgtgcggac gatgatcgga aagccggccg cgcgccggtc gcccgggagc cggcgaatca
     2461 gatccgctgc cgggcccccc ggcgtctccc agaagacgag tttcgaggcg ccgagccgtc
     2521 gcgcttccga cgccgccgca gcgaagagag ccggcagatc ggccccgtcc gcctcgcgcc
     2581 cgaggaaatc cgcgacgagc gccgtgtccc ccgtcacaga gaggaccgcc cagcccacgg
     2641 actctccccg gagctcgcgc gagagcatcc ggtaccagcg ggccggccgt gcgtggaagc
     2701 gccagttcgc gcgcccgcga tcacgcacgg ctgcgtgggt caggaacgtc gcggccctcc
     2761 tccacagcgg gtcgaagctt tccgtcgcgc agtctcccgc caccgcgtcg agagggggcg
     2821 ccgggaacgc atcgcagggg acgagcacct cgcggacggg aaacagcgtc cgcgatccga
     2881 cgatccggtg gctcacctcg agcgcgcgcg gattgggaaa cccgaagcag aagggaactc
     2941 cgcgcgctcc cacggcctcg tagaacgcct cggtcatcgc gcggtagatc ccccgccgcc
     3001 cgcccatccc ccgcacggcg gaatcggtcg caacgtcgcc gacggagtac gagagcgccg
     3061 gccgtccgtc gagaagaagt ctcgtccccc agccggcgta gttgccgacg atctcctccc
     3121 cgacgagggc gacgatcccg aaccagccgt ccggattctg ctcgaacttc cacgtccact
     3181 cggcttcgga catctccttg ccgaagacgc gggcgaagag gcgccggatc ccggaagcgt
     3241 caccggccgt cgcttcccgg atgacgaatc cgctcactcc cgctccgcct cccacaggaa
     3301 agtctcgatc ggatggccgt cggatgcgcg gaacgatcgc agaaagagcg ggaccggggg
     3361 caggcggtcc gcgacctccg aaggcagcac gaagtcccgg tcgagcggcg ctcccgcgaa
     3421 gggaaggagc gacgcgttcg tcgagaccgc ggcccacgcg ccttcccgcg ccgcgagccg
     3481 cggatccagc gttctcggaa aggaaacctc ggaacgccgc cgtcccgtgc gctccagcat
     3541 cgcgccgccg aggcgcccgt agcgccgcct ccagtagagt tgccgcgagc gggcgcggcg
     3601 cgccgcggtc tctccggcgc cggccgcgct cgaaccccag cggtgccgga cgcgcgccga
     3661 gggcacgaat cgcagcgcga gaccgcgagc ccggacgcgg tcctcccact cggtctcctc
     3721 gtactcgaac gggaaccgct cgtcgaaccg cccgacgcgg tcgaaggtct cgcggcgcgc
     3781 cgcgagcacc gtgcccgaga gctggcgcgc ttcgccgccg gactgccaca ggcggagcgt
     3841 ctcccgcgca tgggacgcga aacgcctgtc gtcgcgtgcc ggacgccggc ccgccgaaag
     3901 ctgcgcgagg tccgagcgga aacctggttg ccaacccgga ggcagccgca gacgatcggc
     3961 gtcgtcccag aaggagagag gcgccgccgc cc
//
LOCUS       DASWAV010000682         6875 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U2665
            k141_396078, whole genome shotgun sequence.
ACCESSION   DASWAV010000682 DASWAV010000000
VERSION     DASWAV010000682.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960316
            Sequence Read Archive: SRR10260007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 6875)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6875)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:47:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,929
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6875
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_396078"
                     /isolate="SMAG_U2665"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.36444 N 132.31889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..609
                     /locus_tag="VF958_14370"
     CDS             <1..609
                     /locus_tag="VF958_14370"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEX9150337.1"
                     /translation="HFARLGYDVAALDFRGHGDSGGKYAFGLKEALDVEAVVNELVGA
                     GRPYSRFAIIGLSMGGSIAAQALARFPELPCRALVMISSPADVKTLKPRPLSRDAIRQ
                     LRLRHALRMPRVAIRHLRAPKPNAAQAVERLRMPKLIVTAENDWLVDPSQGRELALRA
                     APPVEHVHLDLPGSLHADALVKYVPLTLLRLLDRWLARNAPP"
     gene            662..1915
                     /locus_tag="VF958_14375"
     CDS             662..1915
                     /locus_tag="VF958_14375"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011683678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-[acyl-carrier-protein] synthase
                     family protein"
                     /protein_id="HEX9150338.1"
                     /translation="MSERHAVITGIGCLSPNGNGREAFGAALKRGDSGIGRISLFDPE
                     GLAATIAGELKGFDTTRWIGAKDIRHVSRAVPMAIAASTEALADAGIEPAKLSLDERR
                     EFGVMLGTGGGAIEFTERMYHLYYTNQTKKASIYAIPSGTIGTLSSELSMEFGLRGLS
                     HVISTGCASSTDALGYAFRAIKHGTNGGVYLAGGVDATVVRGIMEGFVMMRIVSTAWE
                     TEPPRASRPFSADRDGFVLGEGAWMFVIEERDRALARGAKIYGEIRGYGATCDAHHRV
                     RLDETGEEPARAMSLALEEAGLPPEAIDYLAYHGTSTALNDRVETKAVRQAFGVAARK
                     LPGSSIKSMIGHPQGACGAAGVAATLLGMRDSFLPPTINLDTPDEECDLDYVANESRS
                     ASFANALCNCIGFGSKNSALVISRS"
     gene            1925..2623
                     /locus_tag="VF958_14380"
     CDS             1925..2623
                     /locus_tag="VF958_14380"
                     /inference="COORDINATES: protein motif:HMM:NF025033.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEX9150339.1"
                     /translation="MSFLVPPRRPSDEILDDPEIPTEEMYRSLEDLVLVNRWWGSDRA
                     LVRFLVREIRRQGVERPVVLDVGAGSGAVSRRLARRLAGAGHPARVFACDLQWRHLLA
                     GRRITGEAFPSVATDAFGLPFADKSVDWVVSTLFFHHFSPEENVRLLASFERVARRGF
                     ALLDLTRHALPLAFISIAGLLFKTSVSLCDGISSVRQAYTPDEARRIAQDAIPGARVD
                     AVFPFRYLLSRASS"
     gene            2620..3753
                     /locus_tag="VF958_14385"
     CDS             2620..3753
                     /locus_tag="VF958_14385"
                     /inference="COORDINATES: protein motif:HMM:NF013646.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
                     /protein_id="HEX9150340.1"
                     /translation="MSERFDAVVVGAGPAGSSAAAVLADRGHSVLLLEKDVFPRHKVC
                     GEFLSSDALPSLARIRMKESVERATTERMSRGAVHLASGKAVPFDLPAPGIGITRYRL
                     DDLLARRAREAGADVRFNARAVRAAPRPGGGFRVRFVHRQAERDVEAAAVIGAWGRWD
                     ALDRALERGFLGRPSRFFGWNRDFAGRTGFLEGEVRLYLFSGGYCGLSRVEEGTVNLA
                     GVVSEEVWKRAGGGWPAVVDRARSGNPALERDLARMEPGPIGFLGTGPVFFTRKPPSE
                     NGLLMAGDAAGVIDPFSGEGQAAALASGILAGECAERLIAGELSRKECARVYAEEWRK
                     RFARRFAWSAGFRRLMLSRRLGAIAGRLAGEKLVRFAIGATRR"
     gene            complement(3775..4512)
                     /locus_tag="VF958_14390"
     CDS             complement(3775..4512)
                     /locus_tag="VF958_14390"
                     /inference="COORDINATES: protein motif:HMM:NF028441.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein-sorting protein"
                     /protein_id="HEX9150341.1"
                     /translation="MRKLVLSIGLALSALYLGALPGDLQALLAAVDATRNAFDEAVIS
                     ARATQIQDGKESDSADFDIYVKGRDKGLIVFRGGKNGGRKVLTNGERMWLIVPGSRNP
                     VPITANQRLLGGASFGDVARLRFSEDYEARDSGTVESAAGRSCRVLELTAKTAKVTYP
                     RVLLSVDEGEKLPCRVRFFLSSGKETRAVTFTKFRREAGKNVVAEMEVRDLLGAASRV
                     VTRLEYKDFRSARLDPKIFTPEGARGL"
     gene            complement(4548..5771)
                     /locus_tag="VF958_14395"
     CDS             complement(4548..5771)
                     /locus_tag="VF958_14395"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HEX9150342.1"
                     /translation="MSSWALAWRNLVRQKRRTFLMGSVVAFGFAAFALAGGFIAQSFE
                     GLKEGTIRSVGHLQVVDRRAVAGNEEATLEFGLKDAGRARAIAAREPGVDTVLPRIEF
                     VGLATNGTKSVPYLGLGVDPGPEQSSSLAGELVVAGRYLSSADGPDVVLGTGLASALG
                     VKPGDTITLMATTPDGSLNAVDGVVSGLVDVRIKELNDRYLAGGIGLVSRLLETSETV
                     SKLVIFVKKGVDEKRVAPRLERALVAGGFPVAVRRWEDLAVFYGQVKLLYIGIFGFVG
                     AVLIVIVILSAAIVMTMAATERTREIGTLRAIGTRPSGIQKMFLAEGAVLALAGCVAG
                     ALIALVVRTVLNASGLVLPPPPGATHGMPINVRLYPLAYAAGVATMVVTMLVASWFPA
                     RRASRIPIVDALTHV"
     gene            complement(5768..6469)
                     /locus_tag="VF958_14400"
     CDS             complement(5768..6469)
                     /locus_tag="VF958_14400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019576831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX9150343.1"
                     /translation="MASGDLTPLLAARSISKSYRLGAARVPALHDVTLDVRPGEFVAI
                     RGPSGSGKTTLLNLLGLLDVPDAGEVRVGDLDAAGLSENAKSDLRRDRFGFVFQTFNL
                     IPVLTAEENVAYPLALARALPEDRRKRARDLLAAVGCAEKSAVRPDLLSGGQRQRVAI
                     ARALANDPAVVFADEPTANLDSKTADEILDLMRRLNEEHAVAFLFATHDPRVVERAKR
                     VISLTDGRITEDRSR"
     gene            complement(6495..>6875)
                     /locus_tag="VF958_14405"
     CDS             complement(6495..>6875)
                     /locus_tag="VF958_14405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9150344.1"
                     /translation="AQGGRMRGEAGDRYVAEQTVTLRSGKIAAVKESMQRSGELIREG
                     VALMRSYEYKTSKRRLDAAGSEQTAAAQDPMAFAQYRGDSGHAVAAILNAQQGYFEIQ
                     DRDPFSPEIKKVRVVTTVQYFLED"
BASE COUNT         1067 a         2399 c         2322 g         1087 t
ORIGIN      
        1 cacttcgcgc ggctcggcta cgacgtcgcg gccctcgatt ttcgcggcca cggcgacagc
       61 ggcggcaagt acgctttcgg actgaaggaa gcgctcgacg tcgaggcggt cgtcaacgaa
      121 ctcgtgggcg ccggccgacc ctactcgcgt ttcgcgatca tcgggctctc gatgggcggc
      181 tcgatcgcgg cgcaggcgct cgcacgcttt ccggagctcc cctgccgcgc actcgtgatg
      241 atctcatcgc ccgcggacgt caaaacgctg aagccccgac ctttgtctcg cgacgcgatc
      301 cggcaactgc ggctgcgcca cgcgctgcgg atgccgcggg tggcgatccg tcatctgcgc
      361 gctccgaagc cgaacgcggc gcaggccgtg gagaggctcc ggatgccgaa actgatcgtg
      421 accgccgaaa acgactggct ggtcgacccc tcccaggggc gggagctcgc cctgcgggcg
      481 gctccgccgg tcgaacacgt acacctcgac ctgcccggct ccctgcacgc ggacgctctc
      541 gtcaagtacg ttccgctcac gctcctgcgg cttctcgacc gctggctcgc tcggaacgcc
      601 cctccttaag caatcgttga ggcagccggt tcgcacgggg cctgcagcta caatccgcgc
      661 cgtgtcggag cgccatgccg tcatcaccgg gatcggatgt ctttccccga acgggaacgg
      721 ccgggaggcg ttcggcgccg ccctgaagcg gggcgactcg ggaatcggac gcatctcgct
      781 cttcgatccg gaggggctgg cggcgacgat cgccggggag ctcaagggct tcgacaccac
      841 ccgttggatc ggcgcgaaag acatccgcca cgtctcccgc gcggtgccca tggcgatcgc
      901 ggcgtcgacc gaagcgctcg cggacgccgg gatcgaaccg gcgaagctct cgctcgacga
      961 gcgacgggag ttcggagtca tgctcggcac ggggggcggc gcgatcgagt tcaccgagcg
     1021 gatgtaccac ctctactaca cgaaccagac gaagaaagca tcgatctacg cgatcccctc
     1081 cggcacgatc gggacgctct cctcggagct ctcgatggag ttcgggcttc gcggactgtc
     1141 ccacgtcata tcgacgggat gcgcttcctc gaccgacgcg ctcggctacg cgtttcgcgc
     1201 aatcaaacac ggaaccaacg gcggcgtcta tctcgccgga ggcgtggacg cgacggtcgt
     1261 gcggggaatc atggaaggct tcgtgatgat gcgcatcgtc tcgaccgcgt gggagacgga
     1321 gccgccgcgc gcttcccggc cgttctcggc agaccgggac gggttcgtgc tcggcgaagg
     1381 ggcctggatg ttcgtcatcg aggagcggga ccgcgcgctc gcgcgcggcg cgaagatcta
     1441 cggcgagatc cgcggatatg gcgcgacgtg cgacgcgcac caccgcgtgc gcctcgacga
     1501 aacgggcgaa gagcccgccc gcgcgatgtc gctcgctctc gaggaggccg gcctcccccc
     1561 ggaggcgatc gactacctcg cgtaccacgg aacgtcgacc gctctgaacg accgtgtcga
     1621 gacgaaggcg gtgcggcagg ccttcggagt ggcggcccgg aagctccccg gctcgtcgat
     1681 caagtccatg atcgggcatc cccagggcgc ctgcggcgcg gcgggcgtcg cggccactct
     1741 tctcgggatg cgcgactcct tcctgccacc gacgatcaac ctcgacacac ccgacgagga
     1801 gtgcgatctc gactacgtgg ccaacgagtc gcgatcggct tccttcgcga acgcgctctg
     1861 caactgcatc gggttcggat cgaagaacag cgcgctggtg atctcccggt cgtaacggaa
     1921 ggacgtgagt ttcctcgtcc cccctcgccg gccgtccgac gagatcctgg acgatcccga
     1981 gattccgacc gaggagatgt accggtcgct cgaagacctc gtgctcgtca accgctggtg
     2041 gggcagcgac cgggccctcg tgcggttcct cgttcgcgag atccgccggc agggagtcga
     2101 gcgcccggtc gttctcgacg tcggagccgg atcgggcgcc gtttcccggc gcctcgcacg
     2161 caggctcgcc ggggcgggac atccggcaag ggtcttcgcg tgtgacctcc agtggcgcca
     2221 cctcctggcc ggccgccgga tcacgggaga ggcgtttcct tcggtcgcca cggacgcgtt
     2281 cgggcttcca ttcgccgaca agtcggtcga ctgggtcgtc tcgacgctct tcttccacca
     2341 cttctcaccg gaagagaacg tgcgcctgct cgcgtcgttc gagcgggtcg cgcggcgtgg
     2401 gtttgcgctg ctcgacctga cccgtcacgc cctgccgctc gcgttcatat cgatcgccgg
     2461 acttctcttc aagacgagcg tttcgctctg cgacggaatc tcatcggtcc gccaggccta
     2521 cactcccgac gaggcacggc gtatcgcgca ggatgcgatc ccgggcgccc gcgtcgacgc
     2581 cgtctttccg ttccggtatc tcctgtcgag agcgtcgtct tgagcgagag attcgacgcg
     2641 gtcgtcgtcg gcgccggacc ggccggctcg tcggccgcgg cggtactcgc ggaccgcggc
     2701 cactcggtcc tgctgctcga gaaggacgtc tttccgcgcc acaaggtgtg cggcgaattc
     2761 ctctcctccg acgcgctccc cagcctcgcg cggatcagga tgaaggaatc cgtcgagaga
     2821 gcgacgaccg aacggatgag ccggggcgcg gtccacctcg cgtcggggaa agcagtcccg
     2881 ttcgatcttc cggcgccggg gatcgggatc acgagatacc ggctggacga cttgctcgcg
     2941 cggcgcgccc gcgaggcggg cgccgacgtt cgcttcaacg cccgggcggt cagggcggcg
     3001 ccccggcccg gcggcgggtt tcgcgtccgg ttcgtccacc gccaggcgga acgcgatgtc
     3061 gaagcggctg ccgtgatcgg cgcctggggg cgctgggacg ctctcgaccg cgccctcgag
     3121 cgcgggttcc tcgggcgccc gtcccgcttt ttcggatgga accgcgactt cgccggcaga
     3181 acggggtttc tcgagggcga ggttcgcctg tacctctttt ccggaggtta ctgcggtctg
     3241 tcgcgcgtcg aggagggcac agtcaatctc gcgggggtcg tctcggaaga agtgtggaag
     3301 cgtgcgggcg gcggatggcc ggccgtcgtc gatcgggcgc ggagcggcaa cccggcgctc
     3361 gagcgcgacc tggcacggat ggagccgggg ccgatcggtt ttctcggaac gggacccgtg
     3421 ttcttcacgc gcaagccgcc gtcggagaac ggactcctga tggcgggaga tgccgccggg
     3481 gtcatcgatc cgttctcggg cgaaggtcag gcggcggcgc tcgcgagcgg catcctcgcc
     3541 ggcgaatgcg cggaacgcct catcgccggc gagctctcgc ggaaagagtg cgcgcgcgtc
     3601 tacgcagagg agtggcggaa acggttcgcg cgccgtttcg cgtggagcgc cgggttccgc
     3661 cggctgatgc tgagtcggag gttgggcgcg atcgctggcc gactcgcggg agagaagctc
     3721 gtgcggttcg cgatcggtgc gacgcgacgg tagaacgccg acgctccccg cggcctacag
     3781 cccccgcgcc ccctccggcg taaagatctt cgggtcgagc cgcgcgctgc gaaagtcctt
     3841 gtactcgagc cgggtgacga cgcgcgacgc cgcgccgagc aggtcccgca cctccatctc
     3901 cgcaacgacg ttctttcccg cctcacggcg gaacttcgtg aacgtgaccg cgcgcgtctc
     3961 cttcccggag gagaggaaga aacgcactcg gcacggaagt ttttcgccct cgtcgacgga
     4021 aaggagcacg cgcggatacg tgaccttcgc cgtcttcgcc gtgagctcca gaacgcggca
     4081 gctccggccg gcggcgctct cgacggtccc gctatcccga gcttcatagt cctcggaaaa
     4141 ccgcagccgc gcgacgtcgc cgaacgaagc gccgcccagg aggcgctggt tcgccgtgat
     4201 cggcacggga ttccgcgagc cggggacgat caaccacatc cgttcgccgt tcgtcagcac
     4261 cttgcggccg ccgttcttgc cgccgcggaa gacgatgagg cccttgtcgc ggcccttcac
     4321 gtagatgtcg aagtcggcgc tgtcgctctc tttcccgtcc tggatctggg tcgcgcgcgc
     4381 ggagatcacc gcctcatcga acgcgttgcg agtggcgtcc acggccgcca gaagggcctg
     4441 caggtccccc ggcagcgcgc cgaggtacag cgcggaaagc gccaggccga tcgaaaggac
     4501 gagcttcctc acgccgtgga gaacgtcaga aaagccaagg ctgttcctca gacgtgggtg
     4561 agcgcgtcca cgatcgggat tctcgacgcc cgtcgcgccg gaaaccacga ggcgacgagc
     4621 atcgtcacga ccatcgtcgc gacccccgcg gcgtacgcga gcggatacag gcgaacgttg
     4681 atcggcattc cgtgagtggc gcctggcggc ggcgggagaa cgagccccga ggcgttcagg
     4741 acggtgcgta ccacgagcgc gatcaacgcc cccgcgacgc accccgcgag cgcgagcacc
     4801 gcgccctcgg ccaggaacat cttctggata ccggagggcc gcgttccgat cgcgcgcagg
     4861 gtcccgatct cccgggtgcg ctcggtcgct gccatcgtca tcacgatcgc cgcggagagg
     4921 atgacgatga cgatcaggac cgcccccacg aatccgaaga tgccgatgta gagcagcttc
     4981 acctggccgt agaagaccgc gaggtcctcc caccgccgga ccgcgaccgg aaaccccccc
     5041 gcgacgagcg cgcgttcgag ccgcggggcg acccgtttct cgtcgacccc cttcttcacg
     5101 aagatcacga gtttcgagac ggtctcggac gtttcgagca gccgggagac gaggccgatt
     5161 ccgcccgcga ggtaacggtc gttcagctcc ttgatccgca cgtcgacgag acccgacacg
     5221 acgccgtcga ccgcgttcag cgagccatcc ggcgtcgtcg ccatcagcgt gatcgtgtca
     5281 cccggcttga cgccgagggc ggaggcgagg ccggtgccca gcacgacgtc cggcccgtcc
     5341 gcgctcgaga ggtacctgcc ggcgacgacg agctcacccg cgaggctcga cgattgctcg
     5401 ggacccgggt cgacgcccag cccgaggtag gggacggact tcgtgccgtt ggtcgccaga
     5461 ccgacgaact cgatgcgcgg caggacggtg tcgacgcccg gttcacgagc cgcgatcgca
     5521 cgggcccgcc ccgcgtcctt cagcccgaat tcgagggtcg cctcctcgtt gccggcgacg
     5581 gcgcgccggt cgacgacctg caggtgcccg acgctccgga tcgttccctc cttcaatccc
     5641 tcgaacgatt gcgcgatgaa accgccggcg agcgcgaacg cggcgaagcc gaacgccacc
     5701 accgatccca tgaggaacgt ccggcgcttc tgccgaacga ggttgcgcca ggccaatgcc
     5761 cacgagctca ccgagatcga tcctccgtga ttcgaccgtc cgtcagggaa atgacccgct
     5821 ttgcccgttc caccacccgt gggtcgtgcg tcgcgaacag gaaagcgacc gcgtgctcct
     5881 cgttcaaccg ccgcatgagg tcgaggatct cgtcggcggt cttcgagtcg aggttcgccg
     5941 tgggctcgtc cgcgaagacg acggccggat cgttcgcgag cgcccgggcg atcgcgaccc
     6001 gctgccgctg ccccccggag agcaggtccg gccgcaccgc ggacttctcc gcgcacccca
     6061 ccgccgccag caggtcccga gcccgcttgc gacggtcctc cggcaacgca cgcgccagcg
     6121 cgagcggata cgcgacgttt tcctcggcgg tcaggaccgg gatcagattg aacgtttgaa
     6181 aaacgaagcc gaagcgatcg cggcgcaggt ccgacttcgc gttctccgac agtcccgccg
     6241 cgtcgagatc gcccacgcgc acctctcctg cgtccgggac gtcgaggagc ccgagcaggt
     6301 tcaggagcgt cgtcttgccc gacccgctcg gtccccggat cgcgacgaac tctccgggcc
     6361 ggacgtcgag tgtgacgtcg tggagcgcgg gaacccgcgc cgcgccgaga cggtaggact
     6421 tggagatcga gcgcgctgcg aggagcgggg tcaggtctcc acttgccaca tcgagctcga
     6481 gtgagacccg gggatcagtc ctccagaaaa tactgcacgg tcgtgacgac gcggaccttc
     6541 ttgatctcag gggagaaggg atcgcggtcc tggatctcga agtagccctg ctgggcgttg
     6601 aggatcgcgg cgacagcatg tcccgagtca ccgcgatatt gcgcgaaagc catgggatct
     6661 tgcgccgcgg ccgtttgctc acttccggcg gcgtcgagcc gccgcttact tgtcttgtac
     6721 tcgtagctcc gcatgagagc gacgccctcg cggatgagct ccccggagcg ctgcatcgac
     6781 tctttgaccg ccgcgatctt ccccgagcgc agggtcaccg tctgctcggc gacgtagcgg
     6841 tcccccgcct cgccgcgcat gcggccgccc tgcgc
//
LOCUS       DASWAV010000683         7699 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U2665
            k141_359101, whole genome shotgun sequence.
ACCESSION   DASWAV010000683 DASWAV010000000
VERSION     DASWAV010000683.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960316
            Sequence Read Archive: SRR10260007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 7699)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7699)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:47:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,929
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7699
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_359101"
                     /isolate="SMAG_U2665"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.36444 N 132.31889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1544)
                     /gene="nuoL"
                     /locus_tag="VF958_14410"
     CDS             complement(<1..1544)
                     /gene="nuoL"
                     /locus_tag="VF958_14410"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: protein motif:HMM:NF005141.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HEX9150345.1"
                     /translation="MRDLLWLVPLFPLAGFLVNGLVYLASHRTRGSAHHPPADQPAAE
                     SSLETGHPHAHEHLEAHPNFKALHTVVGVGSVGLACLVAFGAIFDVGVKALASGAHHV
                     ITLYRWIPLGVNQSTGQVPGALGEWFVDAALRLDSLSALMIAFVTFVGFLIHVYSVGY
                     MGHDSGYGRYFAYLNLFMFSMLVLVLANNFLLMFVGWEGVGLCSYLLIGYDYHKKFAA
                     DAGKKAFVVNRIGDFGFILGIFGIFSLFGTVDFGKVFAAAAANPSSYAPWMTLVCLCL
                     FLGAVGKSAQVPLYVWLPDAMAGPTPVSALIHAATMVTAGVYMVARCNVLFRLAPDAL
                     LVVAIVGGFTALFAATIGLVQNDIKKVLAYSTVSQLGYMFLACGVGAFAAGMFHVLTH
                     AFFKACLFLGSGSVIHALGGEQDLRRMGGLSRRIPVTYATFLVATLAIAGVPLLAGFF
                     SKDAILAAVFEARFEGVPWLARVLWACALLTAGMTAFYMFRLVSLTFYGEFRGTPDQA
                     AHVHESP"
     gene            complement(1548..1853)
                     /gene="nuoK"
                     /locus_tag="VF958_14415"
     CDS             complement(1548..1853)
                     /gene="nuoK"
                     /locus_tag="VF958_14415"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010163525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoK"
                     /protein_id="HEX9150346.1"
                     /translation="MTITINHVLVVSFLLFSIGLLGVLVRRNFLTVLMSVELMLNAVN
                     LNLVAFSKHLSDLTGQVFAVFVITIAAGEAAIGLALTISLFRQRRTLNVEEANSMQG"
     gene            complement(1855..2424)
                     /locus_tag="VF958_14420"
     CDS             complement(1855..2424)
                     /locus_tag="VF958_14420"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF012709.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit J"
                     /protein_id="HEX9150347.1"
                     /translation="MTGAETVPGALLTGAAGWAFVVFAVLALGMAVAVVVARNPIHSA
                     LFLLSSFLLVACIFVLEQAEFVGAVQILVYAGGIMVLFLFVIMLVHQRTVREMVTFQH
                     QWDVALLFLIVFLVPFLYVLWTERFPDVALNPDAFRTVRGKIAGNTQAVAWVLYRDYL
                     LPFEVASVFLLVAMIGAVVLGKRSAERVD"
     gene            complement(2426..2908)
                     /locus_tag="VF958_14425"
     CDS             complement(2426..2908)
                     /locus_tag="VF958_14425"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024634.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit I"
                     /protein_id="HEX9150348.1"
                     /translation="MALKKWLDKLTLLDLFDGLSVTGRHLFKKKVTIQYPEQKKLPSD
                     RFRGMFRLDEERCIKCTLCAIECPINIIYIDWHNEKNSAGKSVKVLDRFDVDLKRCMF
                     CQLCEEACPTNPKSIWLTTKTYEMAAYDRNDSLYLDIRKLTDWRAAPNVKPFTVVERE
                     "
     gene            complement(2912..4072)
                     /gene="nuoH"
                     /locus_tag="VF958_14430"
     CDS             complement(2912..4072)
                     /gene="nuoH"
                     /locus_tag="VF958_14430"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014264609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoH"
                     /protein_id="HEX9150349.1"
                     /translation="MTPTVLTYVKVVLLVALVPAIVGFVIVLAERRLMGFMQARLGPN
                     RVGPKGLLQGVADLLKLVTKEDITPAQAEKAVYFLAPVLAVIPALTALAVIPFGPAVR
                     IGKIETPLWVSADVNIGLLFLLAISSIGVYGIILGGWASNNKYSLLGGLRSAAQLVSY
                     EVPMGLALVSVLLMTRSLSMVEIVQRQGRLHLWFVFPGLLAFFIYFVAGVAETNRNPF
                     DLPEAESELVSGFNTEYSGVKFAFFFLGEYAAMIVISCVAVTVFFGGWLPPFPHVAAL
                     KFLHVVPPLVWFLIKTGLFLFGYIWFRSTFPRYRFDQLMALGWKWMIPLALGNIVIVA
                     LAILATPGRSNGLVVLSYLFGGLFVVMFLLAKVRQKLARRERLAAIPASASE"
     gene            complement(4078..5157)
                     /locus_tag="VF958_14435"
     CDS             complement(4078..5157)
                     /locus_tag="VF958_14435"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007222721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit D"
                     /protein_id="HEX9150350.1"
                     /translation="MELNFGPQHPSTHGVLRLVIRVDGEKILETKPDVGYLHRGTEKL
                     FETETYPMGIPHTDRLDYVAAATNNHAYCLAIEKLLGIEIPRRAQVIRVILDELQRLS
                     SHLVWLGTSGIDLGAVTPFWYAFREREQILDFFEEYCGARLTLNCMRIGGLPFDLTAG
                     WVERLSEFMDDLPSRIDEYEQMLTDNRIWKKRTVGIGVISGEECIEWGVTGPMLRASG
                     VEWDLRRAQPYECYDELDFAIPTRVNGDTYDRYIVRVQEMRQSVRILKQCIDRLEPGE
                     IRGKVPRVIKPPVEEVYASVESPKGELGFYCVSNGTNRPYRMHVRPPSFINLQALPRL
                     AKGHLVSDLVALIGTVDIVLGEVDR"
     gene            complement(5295..5948)
                     /locus_tag="VF958_14440"
     CDS             complement(5295..5948)
                     /locus_tag="VF958_14440"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF012549.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C"
                     /protein_id="HEX9150351.1"
                     /translation="MADDETTRPPDAVAGAAPPKPAPAAAAQKPAPPKKVEATDASTH
                     PWVASIRSALPDAVVSAKEFARQVTVVVRRERIGEVARHLKDVEDFRYFVDVTAVDWR
                     DRQPRFDVVYHLYSFSKNDRVRVKCAVGDGEEVPSISEVFLAANWPERETWDMFGIRF
                     AGHPDLRRILTWEGFHGFPLRKDFPVEGIDTGAAIYPEEWPEGGGPAADDPNRKAVS"
     gene            complement(5958..6443)
                     /locus_tag="VF958_14445"
     CDS             complement(5958..6443)
                     /locus_tag="VF958_14445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit B family
                     protein"
                     /protein_id="HEX9150352.1"
                     /translation="MPAIIDRFGDNVLTTSVDSVINWARRSSIWPMQFGLACCAIEMM
                     AVMDARHDLSRFGAEVFRGTPRQSDLMIVAGTVVEKMAPIVKRLYDQMPEPKWVIAMG
                     SCATCGGPYRTYAVTQGVDRIVPVDVYVPGCPPRPEALIYGIMALQKKIDRMKVIRKA
                     S"
     gene            complement(6468..6824)
                     /locus_tag="VF958_14450"
     CDS             complement(6468..6824)
                     /locus_tag="VF958_14450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020674776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit A"
                     /protein_id="HEX9150353.1"
                     /translation="MSEYLPILVFALLAAALPLAMIFIAKALQSHRPNPAKLEPYECG
                     VEALSLAHDTRFSVRYFLIAVLFVVFDVETIFLFPWAVMFNRLGIFGLVEMLVFLVIL
                     VVGYVYVWKRKALDWA"
     gene            complement(6858..>7699)
                     /locus_tag="VF958_14455"
     CDS             complement(6858..>7699)
                     /locus_tag="VF958_14455"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014673.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX9150354.1"
                     /translation="AKDAASLEGQLTFIEAGAGEGRFLGDFARALRSESPAVSSRTRL
                     AALERAPAARKKLAASGFDVLSDLPERSVQGWIFANELFDALPVARLRGTREGLEELR
                     VGMEGDRFVWTAAPAPAEWSERLVRFGVALEPGQIAEVRPGAADLHRRLARALDRGRL
                     VLFDYGHRARALYHPLARRAGTLAVHSAGRRGGDPLERPGEQDLTAHVNWDELVAVGE
                     EEGLASEGPMRQARFLARAGVFDFAPGEAEKWRVYRLVDPGGMGEEISVLVQSRHVPP
                     LGF"
BASE COUNT         1480 a         2510 c         2551 g         1158 t
ORIGIN      
        1 ggagactcgt ggacgtgggc ggcctgatcc ggcgtcccgc ggaactcccc gtagaaggtg
       61 agcgaaacca ggcggaacat gtagaaggcg gtcatcccgg ccgtcagcag cgcgcaggcc
      121 cacagaacgc gcgcgagcca cgggacgccc tcgaatcgcg cctcgaagac cgcggcgagg
      181 atcgcgtcct tcgagaagaa accggcgagc aggggcacgc ccgcgatcgc gagcgtcgcc
      241 acgagaaacg tcgcataagt caccgggatg cgccgcgaaa gtccccccat ccgccggagg
      301 tcctgctccc ccccgagcgc atggatgacc gagccggacc cgaggaagag gcaggccttg
      361 aagaaggcgt gcgtcaggac gtggaacatc ccggccgcga aggcgccgac gccgcacgcg
      421 aggaacatgt agccgagctg cgagaccgtc gagtacgcga ggactttctt gatgtcgttc
      481 tggacgagac cgatcgtcgc cgcgaacagg gcagtaaacc caccgacgat cgcgaccaca
      541 aggagcgcgt ccggcgcgag ccggaagagg acgttgcagc gcgcgaccat gtagacgccg
      601 gcggtcacca tcgtcgcggc gtggatgagg gcggagaccg gagtcggtcc cgccatggcg
      661 tccggcagcc agacgtacag aggcacctgc gcagatttcc ccaccgcccc gaggaacagg
      721 cagaggcaga cgagcgtcat ccagggcgcg tacgacgacg ggttcgccgc tgcggcagca
      781 aagaccttcc cgaagtccac ggttccgaaa agggagaaga tcccgaagat cccgaggatg
      841 aatccgaagt ctccgatgcg gttcacgacg aacgccttct tcccggcgtc ggccgcgaac
      901 ttcttgtggt agtcgtagcc gatcaggagg tacgagcaga gtcccacccc ttcccatccg
      961 acgaacatga gcaggaagtt gttcgcgagg acgaggacga gcatcgagaa catgaagagg
     1021 ttcaggtacg cgaagtagcg gccgtacccc gagtcgtgcc ccatgtaacc gaccgagtag
     1081 acgtggatga ggaaccccac gaaggtcacg aacgcgatca tgagggccga gagcgagtcg
     1141 agccgcaggg ccgcgtcgac gaaccactcg ccgagcgcac ccggcacctg cccggtcgac
     1201 tggttcacgc cgagcgggat ccaccggtaa agggtgatca catggtgagc gccggaagcg
     1261 agcgccttca cgccgacgtc gaagatcgct ccgaacgcga cgagacacgc gagaccgacc
     1321 gaaccgacgc cgaccacggt gtggagcgcc ttgaagttcg ggtgcgcctc gaggtgttcg
     1381 tgcgcgtggg gatgtcccgt ctccaggctc gactccgccg ccggctggtc cgccgggggg
     1441 tgatgagcgc ttccccgcgt gcggtgcgag gcgagataga cgagcccgtt gacgaggaat
     1501 ccggcgagcg gaaagagcgg aacgagccag agcagatcgc gcactgctca gccctgcatg
     1561 ctgttcgcct cttccacgtt cagcgtgcgc cgctgtcgga agagggagat ggtgagtgcg
     1621 agaccgattg ccgcttcacc cgcggcgatc gtgatcacga agaccgcgaa cacctggccg
     1681 gtcaggtccg acaggtgctt ggagaaagcg accaggttca ggttgaccgc gttcagcatc
     1741 aactcgacgg acatcagaac cgtgaggaag tttctccgca cgaggacgcc gaggagcccg
     1801 atcgagaaca ggagaaacga gacgacgaga acgtggttga tggtgatcgt cacgctagtc
     1861 cactctctcc gcggaccgct tgcccaggac cactgcgccg atcatcgcga cgagcagaaa
     1921 aacggacgcg acctcgaacg gcaggaggta gtcgcggtag aggacccagg cgaccgcctg
     1981 agtgttgccc gcgatcttcc cccgaacggt gcggaaggcg tccggattca acgccacgtc
     2041 cggaaagcgc tccgtccaca gcacgtagag gaacggcacg aggaacacga tcaggaagag
     2101 gagcgcgacg tcccactggt gctggaacgt gaccatctcg cgcaccgttc gctggtggac
     2161 cagcatgatc acgaagagga agagcaccat gatccccccg gcgtagacga ggatctggac
     2221 ggcgccgacg aactctgcct gctcgagcac gaagatgcag gcgaccagca ggaaggacga
     2281 gagaaggaag agcgccgagt ggatcgggtt cctcgcgacg accacggcaa cggccatgcc
     2341 gagcgcgagc accgcgaaca cgacgaaagc ccagccggcc gcgccggtca ggagagcgcc
     2401 ggggacggtt tccgccccgg tcatctcatt cccgctcgac gacggtgaac ggcttgacgt
     2461 tcggcgccgc tcgccagtcg gtcagtttcc ggatgtcgag gtagagcgag tcgttgcgat
     2521 cgtacgccgc catttcgtag gtcttggtcg tcagccagat cgacttcggg ttcgtcgggc
     2581 aggcctcttc gcacagctgg cagaacatgc agcgcttgag gtcgacgtcg aagcggtcga
     2641 gcaccttgac cgacttcccc gcggagttct tttcgttgtg ccagtcgatg tagatgatgt
     2701 tgatcggaca ctcgatcgcg cagagcgtgc acttgatgca tcgctcctcg tcgagtcgga
     2761 acatcccccg gaaccggtcc gacggcagct tcttctgttc cgggtactgg atcgtcacct
     2821 tcttcttgaa gaggtgccgg ccggtcacgc tcaacccgtc gaagaggtcg aggagcgtca
     2881 gcttgtccag ccacttcttg agagccacgt gttactccga ggccgatgcc ggaatggcag
     2941 ccagccgctc gcgcctcgcc agcttctggc ggaccttcgc gagcaggaac atgacgacga
     3001 acaggccgcc gaagaggtac gacagcacga cgaggccgtt gctccggccg ggggttgcga
     3061 gaatcgcaag cgccacgatg acgatgttcc cgagggcgag cgggatcatc cacttccacc
     3121 cgagcgccat cagctgatcg aagcggtagc gcggaaaagt cgatcggaac cagatgtagc
     3181 cgaagaggaa gagcccggtc ttgatcagga accagaccag cggcgggacg acgtggagga
     3241 acttcaacgc ggcgacgtgc gggaaggggg gcagccagcc cccgaagaac acggtgaccg
     3301 cgacgcacga gatcacgatc atcgcggcgt actcgccgag aaagaagaag gcgaacttca
     3361 cgcccgagta ctcggtgttg aaccccgata cgagctcgga ctccgcctcg ggaaggtcga
     3421 acggattccg gttcgtctcc gcaacgcccg ccacgaaata gatgaagaag gcgagcaagc
     3481 ccgggaacac gaaccagagg tgcagtctcc cctgccgctg cacgatctcc accatcgaga
     3541 gggagcgcgt catgaggagg accgacacga gcgcgagccc catcggaacc tcgtaggaca
     3601 cgagctgcgc tgccgacctc aggccgccca gcagcgaata cttgttgttc gaggcccacc
     3661 ctcccaggat gatcccgtac acgccgatcg acgagatcgc gagcaggaag aggaggccga
     3721 tgttcacgtc ggcggacacc cagagcgggg tctcgatctt cccgatgcgc accgccggcc
     3781 cgaatggaat gaccgccagg gccgtcagcg cgggaatgac cgcgagcacc ggcgcgagga
     3841 agtagaccgc cttctcggcc tgcgccggcg tgatgtcttc cttcgtcacc agcttgagaa
     3901 ggtccgcgac cccctggaga aggcccttcg gtcccacgcg gttcgggccg aggcgggcct
     3961 gcatgaaccc catgagccgc cgctcggcga ggacgatcac gaacccgacg atcgccggca
     4021 ccagcgccac gaggaggacg accttgacgt aggtgaggac cgtcggcgtc aggaacctca
     4081 ccggtccacc tccccgagca cgatgtccac ggttccgatc agcgcgacga ggtcgctgac
     4141 gaggtggccc ttcgcgagcc ggggcagcgc ctggaggttg atgaacgacg gcggccgaac
     4201 gtgcatccgg tacggccgat tggtgccgtt cgagacgcag tagaacccga gctcgccctt
     4261 gggcgactcg accgacgcat acacctcctc gaccggcggc ttgatcacgc gcgggacctt
     4321 gccgcggatc tcgccgggct ccaggcgatc gatgcactgc ttgaggatcc ggacgctctg
     4381 acgcatctcc tggacgcgaa cgatgtagcg gtcgtaggta tcgccgttca cgcgcgtcgg
     4441 gatcgcgaag tcgagctcgt cgtagcactc gtaaggctgt gcgcggcgaa ggtcccactc
     4501 gaccccggac gcgcgcagca tcgggccggt cacgccccac tcgatgcact cctcgcccga
     4561 gatgacgccg atgccgacgg tgcgcttctt ccagatgcgg ttgtcggtca gcatctgctc
     4621 gtattcgtcg atgcgcgacg gaaggtcgtc catgaactcg ctcaggcgct cgacccagcc
     4681 ggcggtgagg tcgaacggga gtccgccgat ccgcatgcag ttcaacgtga gccgggcgcc
     4741 gcagtactcc tcgaagaagt cgaggatctg ctcgcgctcc cggaaggcgt accagaacgg
     4801 cgtgaccgcg ccgaggtcga tccccgaggt cccgagccag accagatgcg aagagagccg
     4861 ctggagctcg tccagaatca cccggatgac ctgagcgcgg cgcgggattt cgatgccgag
     4921 gagcttctcg atcgccaggc agtaagcgtg gttgttcgtc gccgcggcga cgtagtccag
     4981 acggtccgtg tgggggatcc ccatcggata ggtctcggtt tcgaagagct tctcggtccc
     5041 gcggtggaga tagccgacgt ccggtttcgt ctcgaggatc ttctcgccgt ccacgcggat
     5101 gaccagccgc aggacgccgt gagtcgaggg atgctgcggt ccgaagttga gctccatttc
     5161 ctggacttcg aagagcgtgg gcggggtccc ggtcaggggg acgtgctccg ccgaagcgga
     5221 gggcgtcgag gcgacgccgt cggcggtccg catctcctgc gctctctcgg attcggattt
     5281 cgatcgctcg tcgctcacga gactgccttc cgattgggat cgtccgccgc cgggccaccg
     5341 ccctccggcc attcctccgg gtagatcgcc gcgccggtgt cgatcccctc gacgggaaag
     5401 tcctttctca gcgggaatcc gtggaatccc tcccacgtga ggatgcggcg caggtcggga
     5461 tgccccgcga agcggattcc gaacatgtcc cacgtctcgc gctccggcca gttggccgcg
     5521 agaaacactt ccgagatcga cgggacttcc tcgccgtcgc cgaccgcgca cttcactcgc
     5581 acccggtcgt tcttcgaaaa cgagtagagg tggtagacga cgtcgaacct cggctgccgg
     5641 tcgcgccagt cgacggccgt cacatccacg aagtagcgaa agtcctcgac gtccttcaga
     5701 tgccgagcga cctctccaat gcgctcgcga cgcacgacga cggtcacctg cctcgcgaac
     5761 tccttcgcgg acaccacggc gtcggggaga gccgagcgga tcgacgcgac ccacgggtgc
     5821 gtggaggcgt ccgtggcctc gaccttcttg ggaggagccg gcttctgcgc cgccgcggcc
     5881 ggggcgggtt tgggtggcgc ggccccggct acggcgtcgg gaggacgcgt ggtctcgtcg
     5941 tcggccaccg ggttccctta ggacgccttc cggatgacct tcatccggtc gatcttcttc
     6001 tgaagcgcca tgatcccgta gatgagcgct tccggtcggg gcggacaacc cggcacgtag
     6061 acgtccaccg gcacgattcg atccaccccc tgcgtgacgg catacgttcg gtaggggcca
     6121 ccgcacgtcg cgcacgaacc catcgcgatg acccacttgg gctccggcat ctggtcgtag
     6181 agacgcttga cgatcggcgc catcttctcg acgacggttc ccgcgacgat catcaggtcg
     6241 gactgccgcg gagttccgcg aaagacctcg gccccgaaac gggagaggtc gtgccgcgcg
     6301 tccatgaccg ccatcatctc gatcgcgcag caggcgagac cgaactgcat cggccagatg
     6361 gaggaacggc gcgcccagtt gatcaccgag tccacggacg tcgtcaggac gttgtcgccg
     6421 aagcggtcga taatggcagg cactctcttc ggctccctat ccgccggcta cgcccaatcg
     6481 agcgccttgc gcttccagac gtagacgtag ccgaccacga gtatcacgag gaaaaccagc
     6541 atttcgacga gcccgaagat cccgagtcgg ttgaacatga ccgcccaggg gaagagaaag
     6601 atcgtctcga cgtcgaagac gacgaagagg accgcgatca ggaaatagcg gaccgagaac
     6661 cgggtgtcgt gggcgagcga gagggcctcg acgccgcact cgtagggctc gagcttcgcg
     6721 gggttcggac ggtggctctg cagcgctttc gcgatgaaga tcatcgcgag ggggagcgcc
     6781 gccgcgagca gcgcgaacac cagtattggg agatattcgg acaccggcgg cgcattgtat
     6841 cggagggttc cgatcattca aaacccgaga ggcggcacgt ggcgcgattg aacgagcacc
     6901 gagatttctt cgcccattcc tcccggatcg acgagccgat agacgcgcca cttctcggct
     6961 tcgcccggcg cgaagtcgaa gactcccgcc cgcgcgagaa atcgggcctg tcgcatcggc
     7021 ccctcgctcg cgagaccctc ctcctctccg accgcgacga gctcgtccca gttcacgtgc
     7081 gcagtcaggt cctgctcgcc gggccgttcg agcgggtccc ctccgcgccg cccggccgag
     7141 tggaccgcga gcgttccggc acggcgagcg agcgggtgat acagggcgcg ggcgcggtgg
     7201 ccatagtcga agaggacgag ccgcccgcgg tcgagcgcgc gagccagccg gcggtgaaga
     7261 tccgcggccc cgggtcgaac ttcggcgatc tgccccggct cgagcgcgac gccgaaacga
     7321 acgagacgtt cgctccactc ggcgggcgcc ggggccgcgg tccacacgaa ccggtcgcct
     7381 tccatcccca cgcggagctc ctcgagtcct tcgcgtgttc cgcggagtct cgcgaccggc
     7441 aacgcgtcga agagctcgtt ggcgaagatc cagccctgga ccgagcgctc cggcaggtcc
     7501 gacaggacat cgaatccgct cgccgccagt ttctttcgtg ccgccggcgc ccgttcgagg
     7561 gcggccagac gggtcctcga cgagaccgcc ggcgactcgc tccggagggc tcgcgcgaaa
     7621 tcgccgagga acctcccctc tcccgcgccc gcctcgatga acgtgagttg cccttcgaga
     7681 gatgcggcat ccttcgcga
//
LOCUS       DASWAV010000684         5023 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U2665
            k141_396489, whole genome shotgun sequence.
ACCESSION   DASWAV010000684 DASWAV010000000
VERSION     DASWAV010000684.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960316
            Sequence Read Archive: SRR10260007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 5023)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5023)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:47:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,929
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5023
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_396489"
                     /isolate="SMAG_U2665"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.36444 N 132.31889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(138..2795)
                     /gene="bamA"
                     /locus_tag="VF958_14460"
     CDS             complement(138..2795)
                     /gene="bamA"
                     /locus_tag="VF958_14460"
                     /inference="COORDINATES: protein motif:HMM:TIGR03303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamA"
                     /protein_id="HEX9150355.1"
                     /translation="MRQRKRHPARDTRSRAPGARLAPCLVIAVALLLSALRLGAQAPP
                     ARETPAVQPPQPPVPAGAVPGPAVTVAPIPSPTPAPARPAEPTPAPGPAAQLPPAAPA
                     ERIVGIRVVGYQTVSPETIAHYLGVKVGDAYDPEKIRANFQSLWDVGLLENVSVEAER
                     EPAGVTLVVTIEERPTISAIEYSGNKKVSTSDIKDKLKEEKIEINPGAPLSLREVAKT
                     RSAIADLYLEKGFRSATVDYRIEDVSKTDKKVVFVIDEGEKVKIVSIDFEGNTVVSDR
                     RLRMAMKKTKVNVFWRIASDKTTFNQANYDEDVESVKSLYQSKGYKDIVVKDPKIELF
                     VKNPNAKSKKQKRRARITIPVVEGERFYTGDIRVVRVNQQGQPADPPEPTVIPSAQLL
                     REFYELPPGSILNRERLVEALQRIEQRYKSRGYIYWYADPAYHEVADHRVDVEVKLYE
                     GDKFYLGRLEVKGNTTTRDKVIRREFALDEGDVMNMEAVKKSLQKLQQLGYFKISEEP
                     EFSVRPEQKKVDLVIKGTEASKNEVQFGAGYSALDGFFGQFSFQTRNFLGRGEVIGAA
                     AQIGRISKYYDLSYTVPWFMDRNQTVGVSLFRRNVEYLNIDEKRQGGTAFYGKGIGLF
                     DSWSLLYSYEDVKANFPVRGAQVPPGQPAPPEKFTEVTGLTSSLTPGYRYDSRNDPFD
                     PNRGYRLYASVQAAGSVLGGNQEFVKPLIGGSAFVPLRFPRHAYLGLNLEAGYIAPFG
                     GDGIPIFERFQLGGESSLRGFRTGSVLPLKGNDQIFTDEAGRILGGDKYFIFNAEYVF
                     ASLGPAKLLGFADVGNTFHESQAFSLSNVRTSVGAELRIFLPIFQAPLRFIYSFNLDP
                     KTPIDQFGFPINRLKERRSGFDFSIGRTF"
     gene            complement(2897..>5023)
                     /locus_tag="VF958_14465"
     CDS             complement(2897..>5023)
                     /locus_tag="VF958_14465"
                     /inference="COORDINATES: protein motif:HMM:NF019344.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HEX9150356.1"
                     /translation="PYVGTEHLLIGLLRVDGCVAGRLLTARGFNLYGVREETISLIKE
                     REVSKQKKELPFLAEYSRDLTQLAAAGTFDPLIGREKEVERIIQILSRRTKNNPILLG
                     EPGVGKTAIVEGLATRIVEGAVPMFLSTKKILALDLSLIVAGTKYRGQFEERLKGIIR
                     ELRENPDLIIFIDEIHSLIGAGSAEGSLDAANILKPALSRGEVSCIGATTLKEYRKYI
                     EKDRSLLRRFQAIHVTAPSEVETFEILEGVKDRYEQFHKVRYSTEAIKTAVYQSNRYI
                     TDRFFPDKAIDILDEAGAKVKLKRVADTQNLRKLEIETRQIVKEMKKAISDKDFEKAV
                     FLREREIELKEEIERTKAQTADRTEATQEVTRRDIEEIISSWTGIPVASLEMEEAEKL
                     LHMEDALRKRIVGQEAAIIGVSKAIRRSRLGVNNPNRPMGSFIFLGPSGVGKTEVARR
                     LAEFLFENNKALVRFDMSEYMEKHAVSKLIGSPPGYVGHEEGGQLTERIRRNPYSVIL
                     FDEIEKAHPDIANLLLQILEDGILTDAYGNVVDFKNTLIIMTSNIGTKHLVSRTRLGF
                     GGTPERQNMREIEDLVLKELKRDFSPEFINRIDETIVFHPLDREELARICRLLIDDVN
                     VTLAPKGAVLDVDDAAIEWLLQQSGDDPNMGARPLRRAIQKHIEDPVSELLIQSRDER
                     VEAIEVRVGPEGLGVLAREIEPIATE"
BASE COUNT          824 a         1600 c         1614 g          985 t
ORIGIN      
        1 ctcccgaggt aatcgaggga ggagcctcgc gccgcgggag aacgaccgca gcgagggatc
       61 aggcgctcgg tcgaggcttg ctcgccgagc cgagcgccgg tggcgtgagc cagatcccga
      121 gcaagggcgg gcgacggtta gaacgtccgg ccgatcgaga agtcgaaacc ggagcgcctt
      181 tccttcaggc ggttgatcgg aaagccgaac tggtcgatcg gcgtcttcgg atccaggttg
      241 aaggagtaga tgaagcgcag cggcgcctgg aagatcggaa ggaaaatgcg caattccgca
      301 ccgaccgacg ttcgcacgtt cgagagggaa aacgcctggc tctcgtggaa ggtgttgccg
      361 acgtcggcga aaccgaggag cttggccggg ccgagcgacg cgaagacgta ttcggcgttg
      421 aagatgaagt acttgtcccc gccaaggatc cggcccgctt cgtccgtgaa gatctggtcg
      481 ttgcccttga gcggcaggac cgacccggtt cggaatcccc ggagactcga ctcgccgccg
      541 agctggaacc gctcgaagat cggaatgcca tcaccgccga acggcgcaat gtatcccgcc
      601 tcgaggttca agccaaggta cgcgtggcga ggaaagcgga gcggaacgaa cgccgatcct
      661 ccgatgagag gcttgacgaa ttcctggttc ccgccgagca cgctgcccgc cgcctggacg
      721 ctcgcgtaaa gccgatatcc gcggttcggg tcgaacgggt cgttccggct gtcgtagcgg
      781 tatccgggag tcagcgatga ggtcaggccc gtcacctccg tgaacttctc gggaggagcc
      841 ggctgccccg gaggaacctg tgcgccgcgc accgggaaat tcgccttcac gtcttcgtac
      901 gagtagagga gcgaccagct gtcgaagagt ccgatcccct ttccgtagaa ggccgtcccg
      961 ccctggcgct tctcgtcgat attcaagtac tcgacgttgc gccggaaaag ggaaacgccg
     1021 accgtctggt tgcggtccat gaaccacggg acggtgtatg agaggtcgta gtatttcgag
     1081 atccggccga tctgcgcggc ggcgccgatg acctcgccgc gcccgaggaa attccgggtc
     1141 tggaacgaga actgcccgaa gaatccgtcc agcgccgagt agccggcgcc gaactgcacc
     1201 tcgttcttgc tcgcctcggt gcccttgatg acgagatcga ccttcttctg ctcgggccgg
     1261 acggagaact cgggctcttc ggaaatcttg aaatatccga gctgctggag tttctggagg
     1321 ctcttcttga ccgcctccat gttcatgacg tcgccttcgt cgagcgcgaa ctcccgtcga
     1381 atgaccttgt cgcgcgtcgt cgtgttgccc tttacctcga ggcgacccag atagaacttg
     1441 tcgccctcgt agagcttcac ctcgacgtcc acgcggtgat cggcgacctc gtggtaggcg
     1501 ggatccgcgt accagtagat gtagccgcgc gacttgtaac gctgctcgat ccgctggagc
     1561 gcttcgacca ggcgctcgcg gttcagaatg gagcccggcg ggagctcgta gaattccctc
     1621 agaagctgag cggatgggat gacggtgggc tcgggcggat ccgccggctg tccctgctgg
     1681 ttcacccgaa cgacccggat gtcgccggtg tagaagcgct ccccttcgac gacggggatc
     1741 gtgatccgcg cgcggcgctt ctgcttcttc gatttcgcgt tggggttctt gacgaagagc
     1801 tcgatcttcg ggtccttgac gacgatgtcc ttgtagccct tcgattgata gagcgacttg
     1861 acgctctcga cgtcctcgtc gtaattggcc tggttgaagg tggtcttgtc cgaggcgatc
     1921 cgccagaaga cgttcacctt ggtcttcttc atcgccatgc gcaaacgccg atcggatacg
     1981 accgtgttgc cttcgaagtc gatcgacacg atcttgacct tctccccttc gtcgatgacg
     2041 aagaccacct tcttgtcggt cttggagacg tcctcgattc ggtagtcgac cgtcgcgctg
     2101 cggaatccct tctccaggta aaggtcggcg attgcggacc gcgtcttcgc cacctcccgc
     2161 agagagaggg gcgcccccgg attgatctcg atcttctctt ccttcagctt gtccttgatg
     2221 tccgatgtcg aaaccttctt gttgccggag tactcgatcg cggaaatcgt cggccgctct
     2281 tcgatcgtga cgacgagcgt gacgcccgcc ggttcccgct ccgcctccac cgaaacgttc
     2341 tcgagaagac ccacgtccca gagcgactgg aagttcgcgc ggattttctc cggatcgtag
     2401 gcgtctccga ccttcacgcc cagatagtgc gcgatcgttt ccggcgaaac cgtctggtag
     2461 ccgacgacgc ggattccgac gatgcgctcg gccggcgccg cgggcgggag ctgcgcggcc
     2521 gggcccgggg cgggcgtggg ctcggccggg cgcgctggcg cgggagtcgg cgagggaatc
     2581 ggggcgaccg tcacggccgg cccggggacg gcgcccgcgg ggacgggcgg ctgtggcggc
     2641 tgaacggccg gcgtctcgcg cgccggaggt gcctgcgctc cgagccggag ggccgaaagg
     2701 aggagcgcca cggcgatcac gaggcacggc gccagccggg cgccgggcgc ccgggagcgg
     2761 gtgtcgcgag ccgggtgtct tttccgctgc cgcaagcgca atctctatcc tctccgcaag
     2821 cgcgattatt cccgaaggaa cctatcagcc atgggcgaag cgtgggtcgc cgggatgagg
     2881 agccggagct cgcccgctat tcggtcgcga tcggctcgat ttcgcgcgcc agcaccccga
     2941 gcccttcggg ccccacgcga acctcgatcg cctcgacgcg ctcgtcccgg ctctgaatga
     3001 ggagctcgga gacgggatcc tcgatgtgct tctggatcgc gcggcgcagc ggtcgcgcgc
     3061 ccatattggg gtcgtcgccg ctctgctgca gcagccactc gatcgcggcg tcgtcgacgt
     3121 cgagcacggc tcccttgggg gcgagcgtca cgttgacgtc gtcgatcagc agccggcaga
     3181 tgcgggcgag ctcctcgcgg tcgagcgggt gaaacacgat cgtctcgtcg atccggttga
     3241 tgaactcggg agagaaatcc cgcttcagct ccttcaggac gagatcttcg atctcgcgca
     3301 tgttctgccg ctccggcgtc ccaccgaacc cgagtctcgt ccggctcacg aggtgtttcg
     3361 tcccgatgtt ggacgtcatg atgatgagcg tgttcttgaa atcgacgacg ttgccgtacg
     3421 cgtcggtgag gatgccgtcc tccaggatct ggaggagcag gttcgcgatg tccgggtgcg
     3481 ccttttcgat ttcatcgaac aggatgacgc tgtaggggtt gcggcggatc cgctcagtca
     3541 gctgaccgcc ctcttcgtgc ccgacgtagc cgggaggcga gccgatcagc ttcgagacgg
     3601 cgtgcttctc catgtattcc gacatgtcga agcgcaccag cgctttgtta ttctcgaaaa
     3661 ggaactccgc caggcggcgc gccacttcgg tctttccgac cccggacggc ccgagaaaga
     3721 tgaacgatcc catcgggcgg ttggggttgt tcacgccgag ccgggacctg cggatcgcct
     3781 tggagacccc gatgatggcg gcctcctggc ccacgatgcg cttgcgcaga gcgtcttcca
     3841 tgtggagaag cttctcggcc tcctccatct cgagcgaggc aaccgggatt cccgtccacg
     3901 aagaaatgat ctcctcgatg tcgcgccggg tgacctcctg cgtcgcctcg gtccggtccg
     3961 ccgtctgcgc cttcgtcctc tcgatctctt ccttcagctc gatctcgcgc tcgcgcagga
     4021 agaccgcctt ctcgaagtcc ttgtcggaga tcgccttctt catctccttc acgatctgcc
     4081 gcgtctcgat ctcgagcttc cggaggttct gcgtgtcggc gaccctcttc agcttcacct
     4141 tcgcgcccgc ctcgtcgagt atgtcgatcg ccttgtcggg gaagaagcgg tcggtgatgt
     4201 accggttcga ctggtatacg gccgtcttga tcgcctcggt cgaatagcgc accttgtgga
     4261 actgctcgta gcggtccttg accccttcga ggatctcgaa cgtctcgacc tcggacggag
     4321 cagtgacgtg gatcgcctgg aagcgccgca ggaggcttcg atccttctcg atgtacttgc
     4381 ggtactcctt cagcgtcgtc gcgccgatgc aggacacctc gccgcgcgag agcgcgggct
     4441 tcaggatgtt cgcagcgtcg agcgacccct cggcggagcc cgctccgatc aacgagtgga
     4501 tctcgtcgat gaagatgatc aggtccgggt tctcgcgcag ctcgcgaata atccccttca
     4561 ggcgctcctc gaactggccc cggtattttg tcccggccac gatcagcgac agatcgaggg
     4621 caaggatctt cttcgtcgaa agaaacatgg gaacggcgcc ttcgacgatc ctcgtcgcaa
     4681 gaccctcgac gatcgccgtc ttgccgaccc ccggctctcc gagcaggatc gggttgttct
     4741 tcgtgcggcg cgagaggatc tggatgatcc gctcgacctc cttctcgcgg ccgatgagcg
     4801 gatcgaaggt tccggcagcg gcgagctgcg tcaggtcgcg gctgtactcc gcgaggaacg
     4861 gcagctcctt cttctgtttc gacacctccc gctccttgat gagcgagatc gtctcttcgc
     4921 gcacgccgta gagattgaag ccgcgggccg tcaggagccg ccccgccacg cacccgtcca
     4981 cccggaggag cccgatcagc aggtgctccg tgccgacgta cgg
//
LOCUS       DASWAV010000685         1929 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U2665
            k141_440313, whole genome shotgun sequence.
ACCESSION   DASWAV010000685 DASWAV010000000
VERSION     DASWAV010000685.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960316
            Sequence Read Archive: SRR10260007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 1929)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1929)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:47:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,929
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1929
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_440313"
                     /isolate="SMAG_U2665"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.36444 N 132.31889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..749)
                     /locus_tag="VF958_14470"
     CDS             complement(<1..749)
                     /locus_tag="VF958_14470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013578495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0009060 - aerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase subunit I"
                     /protein_id="HEX9150357.1"
                     /translation="MIETPSEPRVNYLNATYGVKSWLLTRDHKRIALLYLASVTVFFF
                     IGGLFALLIRLELLTPAGDLVQSETYNKLFTMHGIVMIFFFLIPSIPAVLGNFLIPMM
                     IGAKDLAFPRLNLLSWYIYVAGGLFTLWAVVHGGVDTGWTFYTPYSTTYSNGYVIVTA
                     VGIFITGFSSILTGLNFIVTIHTMRAPGLTWFRLPLFVWSHYATSLIMVLGTPVIAVT
                     ILMVGVERLFHLGIFDPKLGGDPILFQHLFW"
     gene            complement(770..1768)
                     /gene="coxB"
                     /locus_tag="VF958_14475"
     CDS             complement(770..1768)
                     /gene="coxB"
                     /locus_tag="VF958_14475"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011523798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit II"
                     /protein_id="HEX9150358.1"
                     /translation="MKETFPLFPQQASSLAGRVDALYIYLICVSVVFTLLIAFSILYF
                     AIKYRRRSESELPRGVEGSLTLEIAWSVIPLLIALSFFFWGASVYFAMNRPPNNALEV
                     SVVAKQWMWKVQHADGQREINELHVPVGRPVRLTMTSEDTIHSFYVPAFRIKRDVLPG
                     RVSTMWFQATRTGRFHLFCAEYCGTKHSGMIGWIDVMDPVEFQAWLSGGSGSESLASA
                     GSKLFAQHACNTCHRSDSLARGPNLEGIFGKTVALQDGRTVTADETYIRESIVMPNAK
                     IVAGFQPIMPTFQGLISEEGLLQLVAYVKSLSKAAPSPGGVVPPVNQPSPGPGVAK"
BASE COUNT          397 a          585 c          652 g          295 t
ORIGIN      
        1 aaccagaaga ggtgctggaa gagaatcggg tcgcccccga gcttcgggtc gaagatcccc
       61 aggtgaaaca gccgctccac tccgaccatg aggatcgtca cggcgattac gggcgtgccg
      121 aggaccatga tgaggcttgt cgcgtagtgc gaccagacga agagcggcag ccggaaccag
      181 gtcagcccgg gcgcgcgcat cgtgtggatg gtgacgatga agttcaggcc ggtcaggatc
      241 gacgagaagc cggtgatgaa aatcccgacg gcggtcacga tgacgtagcc gttcgagtac
      301 gtcgtcgagt acggggtgta gaacgtccag cccgtatcga cgcctccgtg aacgaccgcc
      361 cagagcgtga agagcccccc ggcgacgtag atgtaccagg agaggaggtt caggcgcggg
      421 aaagcgaggt ccttcgcacc gatcatcatg gggatcaaga agttcccgag cacggccggg
      481 atcgacggaa tcaggaaaaa gaagatcatg acgatcccgt gcatcgtgaa gagcttgttg
      541 taggtctccg actgcacgag gtcgcccgcc ggcgtcagga gctcgagccg gatcaagagg
      601 gcgaagagcc cgccgatgaa gaagaaaacg gtcacggacg cgaggtacag aagcgcgatg
      661 cgcttgtgat cgcgcgtcag caaccacgac ttcacgccgt aggtcgcgtt caggtagttg
      721 acgcgcggct cggaaggagt ttcgatcacg ggcggagcgg ttacagcgct cacttcgcga
      781 cccccggtcc cggggaaggc tggttgactg gcggcacgac ccccccgggc gacggcgcgg
      841 ccttcgacaa cgacttgacg tacgcgacga gctgcaggag accctcctcg ctgatcaatc
      901 cctgaaacgt cggcatgatc ggctgaaagc cggcaacgat tttcgcattc ggcatgacga
      961 tcgactcgcg aatgtacgtc tcgtcggcgg tgaccgtccg tccgtcctgg agcgcgacgg
     1021 tcttgccgaa gatcccctcg aggttcggcc cgcgcgcgag cgagtcggag cggtggcagg
     1081 tattgcacgc gtgctgcgcg aagagcttcg atccggccga agcgagggac tcgctccccg
     1141 atcctccgga gagccaggcc tggaactcga cggggtccat cacatcgatc cacccgatca
     1201 tcccggagtg cttggtcccg cagtactcgg cgcagaagag gtggaagcgt cccgtgcgcg
     1261 tcgcctggaa ccacatcgtg gaaacgcgtc cgggcagcac gtcccgcttg atgcggaacg
     1321 cgggcacgta gaagctgtgg atggtgtcct ccgaggtcat ggtgagccgc acgggacggc
     1381 cgaccgggac gtggagctcg ttgatctccc gctggccgtc ggcgtgctgg accttccaca
     1441 tccactgctt ggcgacgacc gagacctcga gagcgttatt cggcggccgg ttcattgcga
     1501 agtagacgct cgctccccag aagaagaagg acagcgcgat caggagcgga atgaccgacc
     1561 aggcgatctc gagcgtcagg gacccctcga cgccgcgcgg cagctcggac tctgagcgcc
     1621 ggcggtactt gatcgcgaag tagaggatcg agaacgcgat caggagggtg aagacgacgc
     1681 tgacgcaaat caggtagatg tacagcgcgt cgacccggcc cgcgagcgag gaggcctgtt
     1741 gcggaaagag cggaaaggtc tctttcaccg cgcctccgcc gggcgccgcg cctcgcgccg
     1801 cttttcgcgc cgccacatca cgaaaaggaa cgtcgccaga atcaggacga acgcgacgcc
     1861 tccggcccgc accatgttca tcacgaccgc cccgtatcgc ccggtcgccg ggtcgtagtg
     1921 gtagcagta
//
LOCUS       DASWAV010000686         6228 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U2665
            k141_396585, whole genome shotgun sequence.
ACCESSION   DASWAV010000686 DASWAV010000000
VERSION     DASWAV010000686.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960316
            Sequence Read Archive: SRR10260007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 6228)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6228)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:47:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,929
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6228
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_396585"
                     /isolate="SMAG_U2665"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.36444 N 132.31889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            2..430
                     /locus_tag="VF958_14480"
     CDS             2..430
                     /locus_tag="VF958_14480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008055174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HEX9150359.1"
                     /translation="MRFMVIVKADKDSEAGVMPSEELLAEMGRYNEELAKAGVLLAAE
                     GLHPSSKGARVRFSGSKRTVIDGPFAETKELIAGFWLIQAKSKEEAIEWVKRAPNPFP
                     GAESEVEIRPVFEADDFGSEFTPELREQEERIRAQVAGKK"
     gene            486..1766
                     /locus_tag="VF958_14485"
     CDS             486..1766
                     /locus_tag="VF958_14485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012239033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HEX9150360.1"
                     /translation="MTASDTHRAIDAVWRIESARLIAGLTRMVRDVGLAEELAQDALV
                     IALERWQESGVPDNPGAWLMATAKRRAIDLLRRRTLAERKHQELGRDLEIRQEMAVPA
                     LEATLDDDIGDDLLRLMFIACHPVLSTDARVALTLRLVGGLTTGEIARAFLVSEATIA
                     QRVVRAKRSLAQARVPFEVPRGPDLSARLSSVLEVIYLVFNEGYSATAGDDWMRPALC
                     EDALRLGRILAGLAPREPEVYGLVALMEIQASRLRARTGPSGRPVLLLEQDRARWDQL
                     LIRRGLAALERSEALGGGLGPYALQAAIAACHARAGTPAETDWARIAALYDALTRLAP
                     SPIVDLNRAVAVAMAFGPAAGLEVVDSLTSEPSLAGYHLLPAVRGDLLDKLGRFGEAR
                     AEFQRAASLTRNERERVLLLERAASAGARVSVPP"
     gene            complement(1854..3728)
                     /locus_tag="VF958_14490"
     CDS             complement(1854..3728)
                     /locus_tag="VF958_14490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HEX9150361.1"
                     /translation="MRRSTAFLASVATLAAVSGAALASPPPAPPISGVVRHLEQPISG
                     ALVIFYNLADTSLTRSRSAVDGTFVVASVPVGVYDLIAYKRGFLPSLVRLWHQALPER
                     VSSVEIKLAAAGRGKDGPSTSSTLWDLRDRLPADVLREIALEEVAEAPPASLDRLRLD
                     RLIAGELRTVTDVGGGETSLSRTAVGVRGGLPNGWRYDLHGDYAAVSDSTQPFDETAS
                     GNAAGFALDVATSPIDQVRLSSRRHSIHFGDDSPASLQSHGVSWSRGGREGSVESVAA
                     RYVEETNLYRATALGTSFFPVASRTWEVNAKYARPVDDTPGVSVAMSYRHREASVGPS
                     GVGAEGAFFLTAPDADLSASTALKLFPNTEVEGGVVARYMAGGYGIAPQATVRYDLGH
                     GAVVFVRGLYRVAESGIGNSTVLPRVSSIEESVEAASREGYAVGLERRAGDSSFKFEV
                     STQRIDEAVRAFFEGDLLTDFDSVYFFDGNSVRQIQASARHRLSDTLAGSVAVRYGDI
                     RGGIAPDSATSFGITDSAGHYWSARAAVEVLPTRTGVAILIRGARQSLATSGVSHTND
                     SDKLAISIAQDLSIIGLTPFGSDWKLLLALESARSTAATEREEPISTSRILGGLALAF
                     "
     gene            complement(3826..4725)
                     /locus_tag="VF958_14495"
     CDS             complement(3826..4725)
                     /locus_tag="VF958_14495"
                     /inference="COORDINATES: protein motif:HMM:NF013761.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline dehydrogenase family protein"
                     /protein_id="HEX9150362.1"
                     /translation="MKVVNDLIVATMPLVPRRIVRRIASRYVAGETLGEALETVAALN
                     ADGCMATLDVLGEDVRRREESGDTVAEYLRALDGIAERGVDCNISVKLTALGLKLDPQ
                     HCRREFARVVEGARRHGTFVRIDMEDSSVTEETIRIFGETRARYEKVGLVLQAYLRRS
                     LDDALRAASMHANVRVCKGIYVEPSAIAFQDREEIRDSFSAMVDRLLEAGSYVGIATH
                     DPILVERARATVSRLSLRREAYEYQMLLGVAGDLRRQLVSDGHRLRVYVPYGRAWEAY
                     SMRRLKENPAIAGHVLRGIFMRR"
     gene            complement(4722..5648)
                     /locus_tag="VF958_14500"
     CDS             complement(4722..5648)
                     /locus_tag="VF958_14500"
                     /inference="COORDINATES: protein motif:HMM:NF039185.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HEX9150363.1"
                     /translation="METARTTRANRSFRIIDTCTSRAFSTLHDVRRPHRPRRLARIRG
                     RPEGGRTISVALVLEHIGRAAADSVFPSRCLACDVRPVQRLFRGGVCEECWQAVPRAE
                     GARCDVCDEPLAAAGAGRCGRCLIHPPPFRSLRAAAPYRGSARTILLAFKFRGADYVG
                     PRLAQVSIERLAGDAPFPATVPDEVVAVPATRSAVGRRGYHPAEVLAEAVARSLRIPF
                     AARRLRKVRETARQSGLSLERRRANVRGAFRANGVAPPRVLLVDDVATSGWTARECAA
                     ALLAAGSQDVDVWCFARASRDDVDREEPATQP"
     gene            5602..6183
                     /locus_tag="VF958_14505"
     CDS             5602..6183
                     /locus_tag="VF958_14505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9150364.1"
                     /translation="MIRKLLFALVVLAVSTAPALAQEWGVGASVGLVNDVQHRIRLDG
                     FDPRDASAWVDFQLEDRVLLRGTMGSMKIRGSKAGQFADVSGNAVVLPDLKERIHYVN
                     VAVSYQYWEGDFTSGIFGGIGGYRVDPDSVASELEGFRDPKETVFGLHAGLDGDLRVF
                     SRLSIVGRITFHKLFTDTNRSLLTASAGAAFRF"
BASE COUNT          995 a         2103 c         2184 g          946 t
ORIGIN      
        1 gatgcgattc atggtgatcg tgaaggccga caaggactcc gaggccggcg tcatgcccag
       61 cgaggagctg ctcgccgaaa tgggaaggta caacgaagag ctggcgaaag cgggcgtgct
      121 gctcgcggcg gagggactcc atccgagctc gaagggtgcg cgggtccggt tttcgggatc
      181 gaagcgcacc gtgatcgacg gacctttcgc cgagacgaag gagttgatcg ccggcttctg
      241 gctgatccag gccaaatcga aggaagaggc gatcgaatgg gtcaaacgcg ccccgaaccc
      301 cttccccggc gccgagtcgg aggtcgagat ccgtccggtg ttcgaggcgg acgacttcgg
      361 ttcggaattc actcccgagc tccgggagca ggaggagcgc atccgcgcgc aggtcgccgg
      421 gaagaagtag tcgcggcgcc ggccggcctt ccccgggggc agtgaagatg gtgtgatcgg
      481 aagccgtgac ggcttccgac actcatcgag cgatcgacgc ggtctggagg atcgagtccg
      541 cccgtctcat cgccggcctc acgcggatgg tccgggacgt cggtcttgcc gaggagctcg
      601 cgcaggacgc actcgtcatc gccctggagc gctggcagga gtcgggcgtc ccggacaatc
      661 cgggcgcctg gctcatggcc acggcgaaac gtcgcgcgat cgatctgctg cggcggagaa
      721 cgctcgccga gcgaaagcac caggagctcg gccgcgatct ggaaatccgg caggaaatgg
      781 ccgtgcccgc tctcgaagcg acgctcgacg acgacatcgg tgacgacctc ctgcgcctca
      841 tgttcatcgc ctgtcatccg gtcctctcga ccgacgcgcg cgtcgcgctc acgctgcgcc
      901 tggtcggagg cctgacgacc ggtgagatcg cgcgggcgtt cctcgtctcc gaggcgacga
      961 tcgctcagcg agtcgtccgc gcaaaacggt ccctcgcgca agcgcgggtt ccgttcgagg
     1021 tcccgcgagg ccccgatctc tccgcgcggc tgtcctcggt gctggaagtc atctacctcg
     1081 tcttcaacga gggatactcg gcgacggccg gcgacgactg gatgcgcccg gcgctctgcg
     1141 aggatgcgct ccggctcgga cggatcctcg ccgggctcgc gccgagggag ccggaggtct
     1201 acggcctcgt cgcgctcatg gagatccagg cgtcgcgact tcgagcgcga acaggcccct
     1261 cgggacggcc cgttctcctg ctcgagcagg accgcgcgcg ctgggatcag ctgctcatcc
     1321 gacgcggcct cgccgcgctc gagcgttcgg aagcgctcgg cggcgggctc ggcccctacg
     1381 cactccaggc cgcgatcgcc gcgtgtcacg cgcgggcagg gactcccgcg gaaacggact
     1441 gggctcgcat cgcggcgctg tacgacgcgc tcacccggct cgcgccttca cccatcgtcg
     1501 atttgaatcg tgccgtcgcg gtcgcgatgg cgttcggtcc cgcggcgggc ctcgaggtcg
     1561 tggactccct cacctcggag ccctcgctcg cgggttatca ccttctgccg gccgtacgcg
     1621 gcgacctcct cgacaagctc ggcagattcg gcgaggcacg ggcggagttc caacgcgccg
     1681 catcgctcac ccggaacgaa cgcgagcgcg tgctgctcct cgagcgggcc gcgagcgccg
     1741 gtgcgcgagt atcggtgcca ccgtagcgga gtcccgcaat cgcgccatga acctgagggg
     1801 gatcggagca agaggagacg agcccgggtc cctcgccgcc ggcgcgggtt cgtctagaac
     1861 gcgagcgcga ggccgcccag gatccggctc gtcgagatcg gctcctcgcg ctcggtcgcg
     1921 gcggtcgagc gcgccgactc gagggcgagc agcagcttcc agtcggagcc gaagggcgtc
     1981 agcccgatga tcgagaggtc ctgcgcgatc gagatcgcga gcttgtcgga gtcgttcgtg
     2041 tggctcacgc cgctcgtcgc gagcgactgg cgcgcgccgc ggatgaggat cgcgacgccc
     2101 gtccgggttg gaagcacctc cacggcggcg cgcgcggacc agtagtggcc ggcgctgtcg
     2161 gtgatcccga agctcgtcgc cgagtccggc gcgatccctc cgcggatgtc cccataccgc
     2221 acggccacac tgccggcgag agtgtcggaa agccggtggc gggcggaagc ctggatctgg
     2281 cggacgctgt tgccgtcgaa gaagtacacg ctgtcgaaat cggtcaagag gtcgccctcg
     2341 aagaatgcgc ggaccgcctc gtcgatgcgt tgcgtcgaga cttcgaactt gaacgacgaa
     2401 tcgccggcgc gcctctcgag cccgaccgcg tagccttccc gcgaggcagc ttcgacgctc
     2461 tcctcgatcg aggagacgcg ggggagaacc gtgctgttcc cgattcccga ctcggcgacc
     2521 cggtacaggc cgcgcacgaa gacgacggca ccgtgtccga ggtcgtagcg cacggtcgcc
     2581 tgcggcgcga tgccgtagcc gcccgccatg taacgcgcca cgacgccgcc ctcgacctcg
     2641 gtgttgggga agagcttgag cgcggtcgag gcggacaagt cggcgtccgg cgccgtcagg
     2701 aagaacgccc cctccgcgcc gaccccggac ggaccgacgc tcgcttcgcg gtggcggtac
     2761 gacatggcga ccgaaacgcc gggcgtgtcg tccacgggac gggcgtattt cgcgttcact
     2821 tcccaggtcc gcgacgcgac cgggaagaac gacgtgccga gcgcagtcgc gcggtacagg
     2881 ttcgtctcct cgacgtatcg agcagcgacg gattcgacgc tgccttcgcg gcccccgcgg
     2941 ctccagctca cgccgtggct ctggagactc gcgggcgagt cgtcgccgaa gtggatcgag
     3001 tggcgccggc tcgacaggcg cacctgatcg atcggcgacg tcgcgacatc gagcgcgaag
     3061 ccggcggcgt tgccgctcgc ggtttcatcg aaaggctgcg tggagtcgct cacggccgcg
     3121 tagtcgccgt gcaggtcgta tcgccagccg ttgggaaggc ccccgcgcac accgacggcc
     3181 gtacgcgaca gcgaagtctc gccgccgccc acgtcggtca cggtgcggag ctccccggcg
     3241 atcaggcggt cgagacggag gcggtcgagg gacgccggcg gcgcttccgc aacttcttcg
     3301 agcgcaatct cgcgcagcac gtcggccgga aggcggtcgc gcagatccca cagagttgaa
     3361 gacgtcgagg gcccgtcctt cccccttccg gcggcggcga gcttgatctc gaccgagctc
     3421 acgcgctcgg gaagcgcctg gtgccacagc cgaacgaggg aggggagaaa gcccctcttg
     3481 taggcgatca ggtcgtagac gcccaccggc accgacgcga cgacgaacgt tccgtcgacc
     3541 gcggatcgcg atcgggtcag ggacgtgtca gcgaggttgt agaaaatgac gagggcgccg
     3601 gagatcggct gctcgaggtg ccggacgacg ccggagatcg gtggcgcggg aggaggcgag
     3661 gccaaggcgg cgccgctcac tgccgcgagc gtggcgaccg acgcgagaaa tgcggtgctc
     3721 cttcgcatac acggaccccg aagggcccaa ctccaacgtc gaagtataga attccgcgga
     3781 gtcaatagct attcccccgg ggccgcggcc ccctctcgct aaagcctatc gcctcataaa
     3841 gatccctcga aggacgtggc ccgcgatcgc cgggttctcc ttgagacgtc gcatcgagta
     3901 cgcctcccag gcccggccgt acgggacgta gacgcggagc cgatgcccgt cggaaacgag
     3961 ctgccgccgc agatcgccgg cgacgccgag cagcatctga tactcgtacg cctcccggcg
     4021 aagcgagagt cgcgagacgg tcgcgcgggc gcgctcgacc aggatcggat cgtgcgtcgc
     4081 gattcccacg taggacccgg cttcgagcag ccgatcgacc atcgcagaga agctgtcgcg
     4141 gatctcttca cggtcctgga aggcgatcgc ggacggctcg acgtaaatgc ccttgcagac
     4201 gcgcacgttg gcgtgcatcg aggcggcgcg gagcgcatcg tcgaggctcc ggcgcaggta
     4261 cgcctgaaga acgagaccga ccttctcgta ccgcgcgcgc gtttccccga agatgcggat
     4321 cgtctcttcc gtgacggacg agtcctccat gtcgatgcgg acgaaggtcc cgtgacgtcg
     4381 cgctccctcc acgacccgcg cgaattcgcg acgacagtgc tgcggatcga gcttcaggcc
     4441 gagcgcagtg agcttgaccg aaatgttgca atcgacaccg cgctcggcga tgccgtcgag
     4501 cgcgcgcagg tattcggcga cggtgtctcc ggactcctcc cggcgccgga cgtcctcccc
     4561 gagaacgtcg agcgtcgcca tgcatccatc ggcgttcagt gcggcgacgg tctcgagggc
     4621 ctcgccgagg gtctcgccgg cgacgtagcg tgacgcgatc cggcgcacga tgcgtcgggg
     4681 tacgagaggc atcgtggcga cgatcagatc gttcacgact ttcatggttg cgtcgccggc
     4741 tcctctctgt cgacgtcgtc ccgcgaagcg cgtgcgaagc accacacgtc cacgtcctgc
     4801 gagcccgccg cgaggagcgc cgccgcgcac tcgcgcgccg tccacccgga ggtcgcgacg
     4861 tcgtcgacga gcaggactcg cggcggagca actccgttcg cccggaacgc gccccggacg
     4921 ttcgcgcggc gccgttcgag cgagagtccg ctctgccggg cggtctcgcg gacctttcga
     4981 agacgccgcg ccgcgaaggg gatccgaaga ctccgcgcga cggcctccgc gagaacctcg
     5041 gccgggtgat agccgcgccg tccgaccgcg ctccgtgtcg cgggcacggc gacgacctcg
     5101 tcggggaccg tcgcgggaaa gggagcgtcg cccgccaggc gttcgatcga tacctgcgcg
     5161 aggcgcggcc cgacgtaatc cgctccgcgg aacttgaaag cgaggagaat ggtgcgggcc
     5221 gatcctcgat acggcgccgc ggcgcgcagg gagcgaaacg gcggggggtg gatgaggcac
     5281 cggccgcagc gcccggcgcc ggcagctgcg agcggttcgt cgcagacgtc gcatcgcgcg
     5341 ccttccgctc gcgggacggc ctgccagcac tcctcgcaga cgccaccacg aaacagccgc
     5401 tggacgggtc gcacgtcgca cgcgaggcag cgggacggga agacggaatc cgctgccgcc
     5461 cgaccaatgt gctcgagtac cagtgcgacg gaaatcgtgc gacccccttc cggtcggccg
     5521 cggattctag cgagtcgacg gggacggtgc ggcctccgta catcgtgtag agtcgaaaac
     5581 gctcgggagg tgcacgtgtc gatgatccga aagctcctgt tcgctctggt cgtcctcgcg
     5641 gtctccacgg cgcccgctct cgcgcaggag tggggagtgg gtgcgtcggt gggcctcgtg
     5701 aacgacgtcc agcaccggat ccggctcgac ggcttcgacc cgcgagatgc cagcgcgtgg
     5761 gtggatttcc agctggagga tcgcgtgctg ctgcgcggca cgatggggtc gatgaagatc
     5821 cgggggtcga aggccggtca gttcgccgat gtctcgggca acgccgtcgt tctcccggac
     5881 ctgaaagagc gcatccacta cgtcaacgtc gccgtctcgt atcagtactg ggagggcgac
     5941 ttcacgtccg ggatcttcgg cggcatcggc gggtaccggg tcgatcccga ttccgttgcg
     6001 tccgagctcg aagggtttcg ggaccccaaa gagaccgtgt tcggtttaca cgccggcctc
     6061 gacggagatc tgcgcgtctt ctcgcggctc tcgatcgtcg gccgaatcac gttccacaag
     6121 ctcttcacgg acactaaccg ctcgttgctg accgccagcg ccggggcggc gtttcggttc
     6181 tagttcgcgg gagccggctc tgctgtgatt tccggcgcgt ggcacttt
//
LOCUS       DASWAV010000687         1686 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U2665
            k141_760360, whole genome shotgun sequence.
ACCESSION   DASWAV010000687 DASWAV010000000
VERSION     DASWAV010000687.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960316
            Sequence Read Archive: SRR10260007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 1686)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1686)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:47:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,929
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1686
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_760360"
                     /isolate="SMAG_U2665"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.36444 N 132.31889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            107..412
                     /locus_tag="VF958_14510"
     CDS             107..412
                     /locus_tag="VF958_14510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9150365.1"
                     /translation="MVTFEYRFDIVPGKLEEYDDYLKGTGRNIWLKFPGVKGVRLYRS
                     MLGGSSPQRVVQVDLESLAVLEKILGDPEFRKAKHHFHGLVTHLTDSLLVEVSDKKK"
     gene            complement(426..908)
                     /locus_tag="VF958_14515"
     CDS             complement(426..908)
                     /locus_tag="VF958_14515"
                     /inference="COORDINATES: protein motif:HMM:NF024854.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEX9150366.1"
                     /translation="MASRPSLSETYRTMAHLRRFFESVRRTITTEPTDGGLTPAQQFA
                     LLTIAGNPANKPLTIGDLARLTYATMNTTSALVRRMERARLVRTRRSSKDRRFVYVEL
                     TALGERRLRPSTAAVVRALRTAANEQGRIQLRAAFETWFDNWGAVIKALSSGRRRSRR
                     "
     gene            1049..>1686
                     /locus_tag="VF958_14520"
     CDS             1049..>1686
                     /locus_tag="VF958_14520"
                     /inference="COORDINATES: protein motif:HMM:NF013316.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HEX9150367.1"
                     /translation="MTSLPLLLAPAGSPEAVVAAIDAGADAVYVGLKGWSRGGERREL
                     GWDELVAAQREVVSAGRKLQVAINTIPKPRERAQLFERIPPLLDLGIREVIVNDLGNL
                     MALSRRFPDLRLTASIGCGAQTVADVAFFRDLGADAVVLPGTVSPAEMREIRSVRGIS
                     VEIMVHMVEEFVLLGKCWMPSYVHLKPSPLPAAASAGPGADETPEGALRGLR"
BASE COUNT          290 a          512 c          592 g          292 t
ORIGIN      
        1 gagatcatgt cgatgaactc catggcggcc gatgcccagg agggcatctc cgcattcctc
       61 gggaagaggg cggcctgctg gacgggcaga tagaaaggag agagggatgg ttaccttcga
      121 gtaccgattc gacatcgttc cggggaaact ggaggaatac gacgactacc tcaagggcac
      181 cggcaggaac atctggctga agttccccgg cgtcaaaggc gtccgcttgt accggagcat
      241 gctgggggga tcttcccctc agagggtggt ccaggtcgac ctggagagcc tcgcggtgct
      301 cgagaagatc ctgggagatc ccgagttccg gaaagcgaag caccacttcc acggactggt
      361 cacccacttg actgactcct tgctcgtcga ggtgagcgac aagaagaagt agggagcgcg
      421 cccgcttatc gccgcgagcg ccggcggccc gaagaaagcg ccttgatgac tgcgccccag
      481 ttgtcgaacc acgtctcgaa cgcggcgcgc aactggattc gaccctgctc gttggccgcc
      541 gtgcgcagcg cgcggacgac cgccgcggtg gacggacgaa ggcggcgctc gcccaacgcg
      601 gtcagctcca cgtagacgaa ccgccgatct ttcgaagagc gcctggtgcg cacgagcctc
      661 gcgcgctcca ttcgacgcac gagagccgag gtcgtgttca tcgtggcgta agtcaggcgg
      721 gccaggtcgc cgattgtcag gggcttgttc gcggggttgc cggcgatcgt cagcagggcg
      781 aactgctgcg cgggcgtcaa gcccccatcg gtcggttcag tcgtgatggt ccgtcggacc
      841 gattcgaaga accgccgcag gtgggccatc gtgcggtagg tctccgagag gctcgggcgc
      901 gaggccatct ggggacatta tagggaagcg ctcccgggag gcgttcgggg cccggttgac
      961 acggtgccgg aaggctataa tcactctacg ccatgaatag tcgacggcgc gtactatcgt
     1021 ctccggtgct tccggcttcg gtcgcgggat gacgtccttg ccgctccttc tggcgcctgc
     1081 cgggagtccg gaggccgtcg tggcggcgat cgatgcggga gcagacgccg tctatgtggg
     1141 cctcaagggc tggagccggg gcggggaacg gcgggagctc ggttgggacg aactggtcgc
     1201 cgcacagcga gaggtcgtct cggctggccg gaagctccag gtcgcgatca atacgatccc
     1261 gaagcctcgg gaacgcgctc aactcttcga gcgcatcccc ccgctcctgg atctgggaat
     1321 tcgagaagtc atcgtgaacg acctggggaa tctgatggcg ctctcccggc ggttccccga
     1381 ccttcggctg accgcgagca tcgggtgcgg cgcccagacg gtcgcagacg tcgccttctt
     1441 ccgggacctg ggcgctgatg cggttgtcct ccccgggacg gtgagccccg cggaaatgcg
     1501 tgagatccgg tccgtccgag gaatctcagt ggagatcatg gttcacatgg tcgaggagtt
     1561 cgtcctcctc ggaaaatgct ggatgccgag ttatgtgcat ctgaagccgt cgccccttcc
     1621 ggctgcggct tcggcgggac ccggcgccga cgagacgccg gagggcgctc ttcgcgggct
     1681 gcggca
//
LOCUS       DASWAV010000688         4215 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U2665
            k141_760484, whole genome shotgun sequence.
ACCESSION   DASWAV010000688 DASWAV010000000
VERSION     DASWAV010000688.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960316
            Sequence Read Archive: SRR10260007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 4215)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4215)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:47:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,929
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4215
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_760484"
                     /isolate="SMAG_U2665"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.36444 N 132.31889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1161)
                     /gene="nrfD"
                     /locus_tag="VF958_14525"
     CDS             complement(<1..1161)
                     /gene="nrfD"
                     /locus_tag="VF958_14525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012965163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NrfD/PsrC family molybdoenzyme membrane anchor
                     subunit"
                     /protein_id="HEX9150368.1"
                     /translation="MVERFEHLRAALGQVQGYIYPNEIELHWGLLVVLYPYLTGLVAG
                     AFILASLAKVFRVKEVEPTYRLALLTALAFLLVAPLPLLAHLGHPERSFEIFLTPHFK
                     SAMAMFGFVYAWYLMVVLLLEIWFEYRADLVRWSQKEHGLRRIIHWILSLGSRSLAPE
                     ALAFDKKAVYAITLIGIPSAFFLHGYVGFIFGSVKANPWWSSVLMPIVFLFSAIVSGI
                     AMVLLLYYLTTLVRRRELDMKCLNKLASFLLYALIVDLSLETLDFIHRLYESEESIKI
                     LATLIFSKLFVSLTIVQLLIGTIGPMLLLAAGRFSSLPSELKRVSYFIAALLVQVGIF
                     STRWNVVIGGQLFSKSLRGLTTFKMELGGIEGLFTALALLVLPFVILFVLLKI"
     gene            complement(1164..1925)
                     /locus_tag="VF958_14530"
     CDS             complement(1164..1925)
                     /locus_tag="VF958_14530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011687336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HEX9150369.1"
                     /translation="MAEESTRREVLGQIARFPCAAMLLACCGPLASLAGATERKYNAK
                     DHYYGMGIQVDRCIGCGRCVEACKAENDVPREPFYFRTWVERYQIRAGGEVKVDSPNG
                     GIHSFPPTEEDKDVLRSFFVPKLCNHCDNPPCVQVCPVGATFKSQDGVVLVDKDYCIG
                     CRYCIQACPYGARYLDPRTATADKCTFCYHRITKGLLPACVEACPTGARVFGELKTLA
                     SPLRRMMRMSKLTVLKPSLNTEPKVFYSDLDGEVR"
     gene            complement(1930..2673)
                     /locus_tag="VF958_14535"
     CDS             complement(1930..2673)
                     /locus_tag="VF958_14535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9150370.1"
                     /translation="MRLGKLPDQVIRLGVLGLVAIAVMLYARHRFVPATFGEFGHYRG
                     AAVSAVAAGKIRYAGAAQCAECHDDVAAMKAASYHRTLSCEICHGPAARHAEDPESQK
                     PVIPRERGAACLYCHEYLPSRPTGFPQIIERLHNPVKPCIECHNPHDPKPPKVPESCS
                     ACHAQIYRTKSVSHHANLPCETCHTVDARHREDPRAFLPKKPTSREFCGTCHSKEAKS
                     PPGIPRIDLTTHGGRYLCWQCHYPHFPES"
     gene            complement(2851..3336)
                     /locus_tag="VF958_14540"
     CDS             complement(2851..3336)
                     /locus_tag="VF958_14540"
                     /inference="COORDINATES: protein motif:HMM:NF017015.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HEX9150371.1"
                     /translation="MAERDSFFQMWDREQPKFDRLFDALPVDRLDYRPDPRSRSAGEM
                     VTHLAREQEMLVGMMNDGRIDYRDSVPEDFAEMRALWKKSTEVLRGRLEHLDDAGWNR
                     PVRFFMNGKQINETSTHEMLWGFLHDMIHHRGQLSTYVRSMGGKVPSIYGPSADSPPA
                     G"
     gene            3695..>4215
                     /locus_tag="VF958_14545"
     CDS             3695..>4215
                     /locus_tag="VF958_14545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase S8"
                     /protein_id="HEX9150372.1"
                     /translation="MQAETADGASASVVVFLADQADVRRGYDMKDPDARGWFVYSTLK
                     EHAERTQAGLRAFLDSRGVSFRSYWAANLLIVTADRRLVELLAARGDVARIDSNRPVR
                     GIEDPVVASLRAAPESPDAAEWGVLNVNAPAVWAMGFVGQGMVIAGQDTGIRWTHLAL
                     KNHYRGWNGATAD"
BASE COUNT          767 a         1290 c         1434 g          724 t
ORIGIN      
        1 gatcttcaag agcacgaaca ggatcacgaa cggcagcacg agaagcgcga gcgccgtgaa
       61 aagtccctca attccgccga gctccatctt gaaggtggtc agtccgcgca ggctcttgga
      121 gaagagctgc cctccgatga cgacgttcca ccgcgtgctg aagatgccca cctgcacgag
      181 gagcgcggcg atgaaataag aaacgcgttt caactccgag ggcagggacg agaagcgccc
      241 cgcggcgagc agcagcatcg gcccgatcgt cccgatgagc agctggacga tcgtgaggct
      301 gacgaagagc ttggagaaga tgagcgtcgc caggatcttg atcgattctt cggactcgta
      361 gagacggtga atgaagtcga gggtctcgag ggagaggtcg acgatcaggg catagaggag
      421 gaacgaagcg agcttgttca aacacttcat gtcgagctcg cggcggcgca cgagcgtcgt
      481 caggtaatag agcagcagca ccatcgcgat gcccgagacg atcgccgaga agaggaagac
      541 gatcggcatc aggacactcg accaccaggg attggctttg accgagccga agatgaaacc
      601 gacgtagccg tggaggaaga acgcggaggg aataccgatc agcgtgatcg cgtagacggc
      661 cttcttgtcg aaagccaggg cttccggcgc aaggcttcgc gatccgagcg acaggatcca
      721 gtggatgatg cgccgcaagc cgtgttcttt ctgactccag cggaccaggt cggcccggta
      781 ctcgaaccag atctcgagga gcaggacgac catcaggtac cacgcgtaca cgaagccgaa
      841 catcgccatc gccgatttga agtgcggcgt caggaagatc tcgaaggacc gctcgggatg
      901 gccgagatgc gcgagcagcg gcaggggcgc cacgagcagg aaggcgagcg cggtgagcag
      961 cgccagccgg taggtgggct cgacttcctt gacccggaac accttcgcga gcgaggccag
     1021 gatgaacgcg cctgcgacga gcccggtgag gtaggggtag agaacgacga gcagacccca
     1081 gtgcagctcg atctcgttgg ggtagatgta accctggacc tgaccgaggg ccgcacgcag
     1141 gtgctcgaag cgttcgacca ttcctacctc acttctccgt cgagatcgga gtagaagact
     1201 ttgggctcgg tgttgagcga cggcttcaag acggtgagtt tgctcatgcg catcatccgg
     1261 cggagcgggc tcgccagcgt cttcaactca ccgaacaccc gggcccccgt cgggcaggcc
     1321 tcgacgcagg cgggcagcag acctttcgtg attcggtggt agcagaaggt gcacttgtcg
     1381 gcagtcgccg ttcgtgggtc cagatagcgc gctccgtaag gacacgcctg gatgcagtag
     1441 cggcagccga tgcagtagtc cttgtcgacg agcacgacgc cgtcctggct cttgaacgtg
     1501 gcccccaccg ggcagacctg cacgcagggc gggttgtcgc agtggttgca gagcttgggg
     1561 acgaagaagg agcgaaggac gtccttgtcc tcctcggtcg gcgggaacga gtggatcccg
     1621 ccgttggggc tgtccacctt gacctcgccg ccggcgcgga tctggtatcg ctccacccag
     1681 gtgcggaaat agaacggctc gcgcgggacg tcgttctcgg ccttgcaggc ctcgacgcag
     1741 cgcccgcagc cgatgcaccg gtcgacctgg atgcccatgc cgtagtagtg gtctttcgcg
     1801 ttgtacttgc gctcggtcgc gccggcgaga ctggcgagcg gtccgcagca cgcgagcagc
     1861 atcgccgcgc agggaaagcg tgcaatctga cccaggacct cgcggcgggt gctttcctcg
     1921 gccatgcttt tagctctccg gaaaatgcgg gtagtggcac tgccagcaca gataccggcc
     1981 gccgtgtgtg gtcaggtcga tgcgcgggat ccccggcggg ctcttcgctt ccttcgagtg
     2041 gcaggtgccg cagaactcgc gcgacgtcgg cttcttcggc aggaacgccc ggggatcctc
     2101 gcggtggcgg gcatcgacgg tgtggcaggt ctcacacggg aggttggcgt gatgtgagac
     2161 cgacttggtc cggtagatct gcgcgtggca ggcggagcag gactccggca cctttggcgg
     2221 cttcgggtcg tgcgggttgt gacattcgat gcacggtttc accgggttgt gcaagcgctc
     2281 gatgatctgc gggaacccgg tcggccggga cggcaggtac tcgtggcagt agaggcaggc
     2341 cgcgccccgc tcgcgcggga tgacgggttt ctgcgattcg gggtcctccg cgtgtcgggc
     2401 ggccggaccg tggcagatct cgcaggagag cgttcgatgg taggacgcgg ccttcatggc
     2461 agcgacgtcg tcgtggcact cggcgcactg cgccgctccc gcgtaccgga tcttgccggc
     2521 ggcaaccgcg ctgaccgcgg cgccgcggta gtggccgaac tcaccgaagg tcgcgggcac
     2581 gaagcgatgt cgggcgtaga gcatgacggc gatggcgacg agaccgagaa cgcccagccg
     2641 tatgacctga tccggcagct tccccaggcg caagcttctc cctcgcggcg tcgcacgcct
     2701 tccgcaaact cgcggggagc ggattgcacg ccgtctgatc tgtggaaatc agcttgacga
     2761 ggcggagcgc gggatcatca gatgcaaatg cattactgga agacgtgctt cacgccaacg
     2821 tctcccgatg cgcggggcga gggagagcgg ctatccggcc ggaggcgaat ccgctgacgg
     2881 tccgtagatc gaagggactt tgcctcccat cgagcggaca tatgtcgaga gctggccgcg
     2941 gtggtggatc atgtcgtgca gaaaccccca gagcatctcg tgcgtcgatg tctcgttgat
     3001 ctgcttgccg ttcatgaaga acctgactgg ccggttccat ccggcgtcgt cgaggtgctc
     3061 cagccgtcct ctcagcactt cggtgctctt cttccacagg gcacgcatct ccgcgaagtc
     3121 ttcggggacc gaatccctgt agtcgatcct cccgtcgttc atcatcccca cgagcatttc
     3181 ctgctcgcgc gcgagatggg tcaccatttc gcccgcggat cgcgacctgg gatccgggcg
     3241 gtagtcgagc cggtcgaccg ggagcgcgtc gaagagccga tcgaacttcg gctgctcccg
     3301 atcccacatc tgaaagaatg aatcgcgttc tgccactcga gcctcctcgt ccccccgatt
     3361 ctatgtgttc ggctccgcct cattcgctgg cgaggagcac tggctcgtaa ccgcgcaccg
     3421 cgtcattcga tgatcgtttg ggagggtgac cgtggagagc gcccttgcca atccgcgttg
     3481 acaccctgga tgaacccgcg tagggtgccc gtctgacgga ccccggatgg gtgacgtaag
     3541 aagccgaatc ggagcgagcc tcgtcgcgct tggtgtgatg ctcccggcgg ccgtcgccgt
     3601 gttgcctcgg agcccggccc aggctgccag gacctctccg ggtcccggga gtcgactctc
     3661 gctggacaag cccgcgggca agatcgcgcc ccaggtgcag gctgagaccg ccgacggggc
     3721 gagcgcgtcc gtggtcgtgt ttctcgccga ccaggcagac gtccgcaggg gctatgacat
     3781 gaaggatccg gatgcccgcg gctggttcgt ctactcgacg ctgaaagagc acgccgaacg
     3841 gacgcaggcc ggattgagag cgttcctcga ctcccggggc gtgagtttcc ggtcctactg
     3901 ggcggccaat ctgttgatcg tgacggccga caggaggttg gtcgaactcc tcgcggcccg
     3961 cggcgacgtc gcgcgcatcg attcgaaccg acccgtccgg ggaatcgaag atcccgtcgt
     4021 ggcgtctctg cgcgcggcgc ccgaaagccc cgatgccgcc gaatggggcg ttctgaacgt
     4081 caacgctccg gccgtctggg cgatgggatt cgtcggccag ggcatggtca tcgccggcca
     4141 ggacaccggc atccgatgga cccacctcgc cctcaagaat cactaccgcg gctggaacgg
     4201 cgcgactgcg gatca
//
LOCUS       DASWAV010000689         6659 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U2665
            k141_596531, whole genome shotgun sequence.
ACCESSION   DASWAV010000689 DASWAV010000000
VERSION     DASWAV010000689.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960316
            Sequence Read Archive: SRR10260007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 6659)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6659)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:47:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,929
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6659
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_596531"
                     /isolate="SMAG_U2665"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.36444 N 132.31889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1702
                     /locus_tag="VF958_14550"
     CDS             <1..1702
                     /locus_tag="VF958_14550"
                     /inference="COORDINATES: protein motif:HMM:NF013727.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HEX9150373.1"
                     /translation="RTVVAKHLEKARRDGISLDDIPFASEDSPPFLRELPGPGKFAIT
                     KKSAESVRPSAPKRKPSAPPQQNLPISVEGYTFPPISLLEKREGAGAVDRRVLVETGR
                     QIAEKCAEFGVEGEVVEYHPGPVVTTYEFRPAAGIKVNQVMGLSEDLALALSAESIRI
                     ERLPGRASVGIEVPNPGGGEIIALRDVIESDRFQSSPSLLTLALGKDIHGEPVVADLR
                     AMPHLLIAGTTGSGKSVAINALITSILFKARPDEVKMILIDPKMVELEVYEDLPHLST
                     KIIIDPKKAANALKWAVDVMEQRFQTLSDVGQVRNAEQYNAAIRDPKTVARVRAEHPE
                     DPDLKLEPMFLIVIVIDELADLMMTAPKEVEEAIVRLAPKARAVGIHLVVATQRPSVD
                     ILTGVIKANLPSRIAFKVSSKIDSRVILDGNGAERLLGRGDMLFLAPGTSRLRRVHGA
                     FVSVQETAGLVRILKKQAKPEYDDQITKDRSEVERRGEDADDETDPLYDEAARLVVRE
                     KMASISFLQRRMGVGFSRAGKLIDMMTRDGLLGPQRGSKPREVLVADDYFEEVDRQPR
                     "
     gene            1863..2801
                     /gene="fmt"
                     /locus_tag="VF958_14555"
     CDS             1863..2801
                     /gene="fmt"
                     /locus_tag="VF958_14555"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR00460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HEX9150374.1"
                     /translation="MRVVFFGTPRFAVPSLRALFDAGFEIPLTVTQPDKPVGRSGQPR
                     LSPVAELAEALRLPVEKPDRLRGNAAFLERLAETRPDAIAVVAYGKLLPSEILELPPL
                     GCVNVHASLLPRHRGASPVQAAILAGDAETGVVTMRIVPELDAGPIYLESRTKIGERD
                     TTETLGGRLARSGAELLIATLRGLEEGTLVPIPQIGEATFTRLIRREDARVDWDEPAP
                     AIDRRLRAFTPWPGLYATLGGERIKLLELEPAGKILSAQEPGMIWEEWGKTMVVAGQG
                     TALFLKTVQRAGRRPVTGSEFLRGLSFRPVRFEPAS"
     gene            2801..4120
                     /locus_tag="VF958_14560"
     CDS             2801..4120
                     /locus_tag="VF958_14560"
                     /inference="COORDINATES: protein motif:HMM:NF013362.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HEX9150375.1"
                     /translation="MASRPPSAVRVRAVEILRRAIRRGAEVPPLLALREGALSPADRD
                     LLREIVYGVLRSRAALDAELASVSRAPLSRLEGDLREILEVALYQVRRLDRVPAYAAV
                     DEAVRHARASGGERAGKLVNAVLRKLASRPSPPAGRGTSVAELARETSHPEFLVRRWI
                     DRFGRDASVRILDADNAPSAIDLLVNPRRTDRETLRQSLAAEGVGTEASDVSPLALTV
                     VAGNPLGSALLAAGHFTVQDVGAQALALLMPPGDLLVDLAAAPGGKTFAALALGGARC
                     AISLDRSIGRLVRFTHNARRLGMPEARAVGGDFLAPPLRRAHFDRVILDAPCSGTGTL
                     RKNPEIRYRLSPAGIDRLARAQVEAVSAAADLLATGGYLLYSTCSLEEEENERVIEAV
                     LAKRPGLSLAPIDALGPLARFVEGARLRLFPDARTDGFTAHLLRRRS"
     gene            complement(4121..4714)
                     /locus_tag="VF958_14565"
     CDS             complement(4121..4714)
                     /locus_tag="VF958_14565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9150376.1"
                     /translation="MFRRTALRSLVFVVAFCGAVPRPARAEGDRRGNLTGTWRLNHDL
                     SDNPERMREAGPVGGTRSGRGGFGGRRGGGFGGHRGRREEVDDGAPPALDPEALETLT
                     IVHRDPEFRITDGLGRQHVLFTDGRKVKEERSAGTVKIRTDWKDGHVVVTTTPKRGPR
                     ITEAYGVAADGSVLTVTTRVEGRGPSLELRRVYDAVR"
     gene            4866..5984
                     /locus_tag="VF958_14570"
     CDS             4866..5984
                     /locus_tag="VF958_14570"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015940721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HEX9150377.1"
                     /translation="MSSPPAVPLLDLTRYDEELQHQISSSVAEVFSSGRFILGPPNVE
                     FEKALAARLGVKHALGVSSGTDALLVSLMALGIGPGDEVVTAPFSFFASAGVIARLGA
                     RPVFADIEAETFNLDPDRLAAALSPRTRAVMPVHLYGQCADMDPILEVADKIPIVEDA
                     CQAVGATYRDRAAGSLATIAAFSFYPTKNLGGAGDSGAVTTNDDSLAARVASLRMHGE
                     AERYHHSSVGGNFRMDSIQAAVLLAKLPRLDSWNQRRIAIAQRYGELLWDAEMRGRVA
                     LPSLAPGRRHVFHQYVVRVRERDRVRAKLAERGISTAVFYPIPLHLQECFADLGYKAG
                     DFPEAEKAAKEVLALPMFPQLTDAEVERVAEGVLAALV"
     gene            complement(5981..>6659)
                     /locus_tag="VF958_14575"
     CDS             complement(5981..>6659)
                     /locus_tag="VF958_14575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX9150378.1"
                     /translation="TGDAAAVLSADLQEPPELLLQLVEAWRGGATAVLAVRSARPEPW
                     ATRAAATVYYRTLRRLALGSMPAGGFDCFLIGRPAIDFLSGGREVHTSLPGLILWAGF
                     PTALVSYERAPRQEGKSRWTLAKKVKLFLDSVISFSYAPLRWMSVFGAILALLAFAYA
                     ALLVVLKVFQDQPIRGWTSLMVALAFFSGVQLLSLGILGEYLWRTLDAARARKGFLVR
                     ERTPENG"
BASE COUNT         1022 a         2323 c         2270 g         1044 t
ORIGIN      
        1 ccgcacggtc gtcgcgaagc acctcgagaa ggccaggcgc gacgggatct cgctcgacga
       61 catcccgttc gcgtccgaag actcgccccc gtttctgcgc gagttgcccg gccctggaaa
      121 gttcgcgatc acgaagaaat cggccgagtc ggtccggcct tccgccccga agaggaagcc
      181 atcggcgcct ccgcagcaga acctgccgat ctcggtcgag ggctacacct ttccgccgat
      241 ctcgctgctc gagaagcggg agggggcggg cgccgtcgac cgtcgcgtcc tcgtcgagac
      301 cgggcggcag atcgccgaaa agtgtgcgga gttcggcgtc gaaggggagg tcgtcgagta
      361 tcaccccggg cccgtggtca cgacctacga gttccggccg gcagccggca tcaaggtcaa
      421 tcaggtcatg ggcctttcgg aggacctcgc actcgccctt tcggccgagt cgatccgcat
      481 cgaacggctg ccgggacggg cgagcgtggg catcgaggtc cccaaccccg gcggcggcga
      541 gatcatcgcg ctccgcgacg tgatcgagtc ggaccgcttt caatcgtcgc cgtccctcct
      601 gacgctcgcg cttggcaagg acatccacgg agagccggtc gtcgccgacc ttcgggccat
      661 gccccacctc ctcatcgcgg gcacgacggg atccggaaag agcgtcgcaa tcaatgcgct
      721 catcacgtcg attctcttca aggcaagacc cgacgaggtc aagatgatcc tgatcgaccc
      781 gaagatggtc gagctcgagg tctacgagga cctcccgcac ctctcgacga agatcatcat
      841 cgacccgaag aaggcggcga acgcgctgaa gtgggcggtc gacgtgatgg aacaacgctt
      901 ccagaccctc tccgacgtcg gccaggttcg gaacgcggag cagtacaacg ccgcgattcg
      961 cgacccaaag acggtcgcac gggtccgcgc cgagcacccg gaagaccccg acctcaagct
     1021 cgagccgatg ttcctgatcg tcatcgtcat cgacgaactc gcagacctga tgatgacggc
     1081 gccgaaggag gtggaggagg cgatcgtgcg cctcgcgccg aaagcgcgcg cggtcgggat
     1141 ccatctcgtc gtggccacgc agcgcccctc ggtcgacatt ctgaccggcg tgatcaaggc
     1201 aaacctgccc tcccggatcg cattcaaggt ttcatccaag atcgactcgc gggtcatcct
     1261 cgacggcaac ggcgccgagc ggctcctcgg gcgcggcgac atgctgttcc tggcgccggg
     1321 tacttcgcgc ctgaggcgcg tgcacggcgc ttttgtttcg gtgcaggaga ccgccgggct
     1381 cgtgcgcatc ctcaagaagc aggcgaaacc cgagtacgac gaccagatca ccaaggatcg
     1441 ttccgaggtc gagcgtcgcg gcgaggacgc cgacgacgag accgacccgc tctacgacga
     1501 ggccgccagg ctcgtcgtga gggaaaagat ggcgtcgatc tccttcctgc agcggcggat
     1561 gggcgtcggt ttctcgcgcg ccgggaaatt gatcgacatg atgacccgcg acggcctcct
     1621 cgggccgcag cgcggatcga agccccgcga ggttctcgtc gccgacgact acttcgagga
     1681 agtggaccga cagccgcgat aagccgtcgc tcccgctccc gcagcgtgag gctcctccct
     1741 tcgggagact cctcacgctg cgaccgggag actcctcgcg ccgcgagccc agccgggcga
     1801 atccatcggc tcgggagact cttcgcgatg cgaatccaga agtctcattt aggcttgacg
     1861 gcatgcgcgt cgtctttttc gggacgcccc ggttcgcggt gccgtcgctc cgggcgcttt
     1921 ttgacgcggg cttcgagatt ccgctcaccg tcacgcagcc ggacaagccc gttggccgaa
     1981 gcgggcagcc tcgcctgtcg cccgtggcgg agctcgccga agcgctccgg ctgcccgtcg
     2041 agaagccgga tcggctgcgc ggaaacgccg cattcctcga gcgcctcgcg gaaacgcgac
     2101 ccgacgcaat cgcggtcgtc gcgtacggga aactcctgcc gtcggagatc ctcgaactcc
     2161 ctccgctcgg ctgcgtgaac gtgcacgcct cactccttcc gcgtcaccgc ggcgcgtcgc
     2221 cggtccaggc ggcgatcctc gcgggcgacg cggagaccgg ggtggtcacc atgcggatcg
     2281 tccctgagct cgacgcgggt ccgatctacc tggagagccg gacgaagatc ggcgagaggg
     2341 ataccaccga gacgctcggg gggcgactgg cgcggtcggg cgcggagttg ctcatcgcga
     2401 cgctgcgcgg cctcgaagag ggcacgctcg tcccgatacc tcagatcggc gaagcgacct
     2461 tcacgcggct gatccggcgc gaggatgctc gggtggattg ggacgagccg gccccggcca
     2521 tcgatcggcg cctgcgcgct ttcactccct ggcccggtct ctatgcgacg ctcggcggag
     2581 aacgcatcaa gctgctggag ctcgagccgg ccgggaagat cttgtcggcg caggagcccg
     2641 ggatgatctg ggaggagtgg ggaaagacga tggttgtcgc cggtcaggga acggcgctct
     2701 ttctgaagac ggtccagcgc gccgggcgcc gcccggtgac cgggagcgag ttccttcgag
     2761 gactttcgtt ccgcccggtc cgattcgaac cggcgtcctg atggcctccc ggccgccgtc
     2821 ggccgtccgg gtgcgcgccg tcgagatcct ccgccgggcg atccgtcgcg gcgccgaggt
     2881 gcctccgctc ctcgctctcc gcgagggggc gctgtcgccg gccgaccggg acctgctgcg
     2941 ggaaatcgtc tacggcgtgt tgcgctcccg cgccgcgctc gacgcggagc ttgcgtcggt
     3001 ctcgcgcgcg ccgctctcga gactggaggg cgacctgcgc gagatcctcg aagtcgcgct
     3061 ctaccaggtc cggcgcctcg accgggtccc cgcgtatgcc gccgtggacg aagcggttcg
     3121 tcacgcgcgc gcgagcggtg gggagcgagc cggcaagctc gtgaacgcgg tgctccggaa
     3181 gctcgcgagc cgcccgtccc cgccggcggg acgaggaacg agcgtcgccg agctcgcgcg
     3241 cgagacctcg catcccgagt ttctcgtacg ccggtggatc gatcgctttg gccgcgacgc
     3301 ctccgtccgc atcctcgacg cggacaacgc gccctccgcg atcgatctgc tcgtcaaccc
     3361 ccggcgcacg gatcgagaga cgctgcggca gtcgctcgcg gcggaaggag tcggaaccga
     3421 ggcctcggat gtttcgccgc tcgccctgac cgtcgttgcg gggaatccgc tcggctccgc
     3481 gctcctggca gccgggcact tcacggtcca ggacgtcggc gcgcaggcgc tcgccctcct
     3541 gatgccgccg ggagatctcc tcgttgatct cgccgcggcg cccggcggca agacgttcgc
     3601 cgcgctcgcg ctcggcggcg cgaggtgcgc gatctcgctc gaccgttcga tcgggcgact
     3661 cgttcgcttc acccacaacg cgcgacgtct cgggatgccc gaggcgcgcg ccgtgggagg
     3721 ggacttcctc gcgcccccgc ttcggagggc gcatttcgat cgcgtgatcc tcgacgcgcc
     3781 ctgcagcggc acgggaacgc tgcgcaagaa cccggagatc cgttaccgcc tgagcccggc
     3841 cggaatcgac cggctcgccc gtgcgcaggt cgaggctgtg tcggccgcgg cggatctgct
     3901 ggcgaccggc ggttacctgc tctattcgac gtgcagcctc gaggaggagg agaacgagcg
     3961 cgtgatcgag gccgtgctcg cgaagcgtcc cgggctgtcg cttgcgccga tcgacgccct
     4021 cgggccgctc gcgcggttcg ttgagggcgc gcggctgcga ctctttccgg acgcgcgcac
     4081 cgacggcttc accgcgcacc tgctccgcag acggagctag ctagcgcacc gcgtcgtaga
     4141 cccggcgaag ctcgagactc gggccgcgcc cttcgactcg cgtcgtcacg gtgagcacgg
     4201 agccgtcggc cgcgacgcca tacgcttccg tgatcctggg accgcgcttg ggagtcgtcg
     4261 tcacgaccac gtgaccgtct ttccagtccg ttcggatctt gaccgtgccg gcggagcgct
     4321 cctctttcac cttgcgtccg tccgtgaaga ggacgtgttg gcgcccgagg ccgtcggtga
     4381 tgcggaactc cgggtcgcgg tggacgatgg tgagcgtctc gagggcctcg gggtcgagcg
     4441 cgggcggtgc tccgtcgtcg acctcttctc gccgcccgcg gtggcctccg aacccacccc
     4501 cccgtcggcc tccgaaccct ccccgcccgg agcgcgttcc tccgacgggc ccagcctcgc
     4561 gcatccgctc ggggttgtcg gagaggtcgt ggttgagccg ccaggttccg gtcaggttcc
     4621 cgcggcgatc cccttccgcg cgggccggcc gtgggacggc gccgcaaaac gcgacgacga
     4681 agacgagcga tcgaagagcg gtgcgccgga acacggagcc tccttctcga ctggaagtga
     4741 tcgccggcgc gcccttccgg tcatcacacc ttcgcaatcg aagtaccggc ctgtaaattc
     4801 cggcgacaac gggcggttcg ctcgcccggc gggccccgac ctttttcggt cggtagaatg
     4861 cggcggtgag ctcgccgccc gccgttcccc tgctcgacct cacgcgctac gacgaggagc
     4921 tgcagcacca gatctcgtcg tcggtcgccg aagtcttttc ctccgggcgg ttcatcctcg
     4981 ggcctcccaa cgtcgagttc gaaaaggcgc tcgcggcacg gctcggcgtc aagcacgcgc
     5041 tcggggtttc ctcggggacc gatgctctgc tcgtttcgct gatggcgctc ggaatcggcc
     5101 ccggcgacga ggtggtgacg gctccgttca gcttcttcgc ctcggcgggc gtcattgcgc
     5161 ggctgggcgc gcgaccggtg ttcgcggaca tcgaggccga gacgttcaac ctcgacccgg
     5221 accgtctcgc ggcggcgttg agcccccgca cccgcgcggt gatgccggtg cacctctacg
     5281 ggcagtgcgc ggacatggat ccgattctcg aggtcgcgga caagatcccg atcgtcgaag
     5341 acgcctgcca ggcggtcggc gcgacttatc gggaccgcgc cgcagggtcg ctcgcgacga
     5401 tcgcggcgtt ctcgttctat ccgacgaaga acctcggagg cgccggcgac tcgggggcgg
     5461 tcacgacgaa cgacgactcg ctcgccgctc gcgtcgcttc gctgcgcatg cacggcgagg
     5521 ccgagcgcta tcaccactcg tccgttggag ggaacttccg gatggactcg attcaggcgg
     5581 ccgttcttct cgcgaagctt ccgcggctcg attcgtggaa tcagaggcgg atcgcgattg
     5641 cgcagcgcta cggagagctg ctctgggacg ccgagatgag gggccgggtc gccctgccgt
     5701 cgctcgcgcc ggggcgccga cacgtctttc accagtacgt cgtacgggtc cgagagcgcg
     5761 accgcgtgcg cgcgaagctc gccgaacggg gcatctccac ggcggtcttt tatccgatcc
     5821 ctcttcacct ccaggagtgc ttcgcggacc tcggctacaa ggccggggat tttccggaag
     5881 cggagaaggc cgcgaaggag gtcctggcgc tcccgatgtt cccccagctc acggacgcgg
     5941 aggtcgagcg cgtcgcggag ggagttctcg cggcgctcgt ctagccgttc tccggcgttc
     6001 gctcccgcac gagaaatcct tttcgcgcgc gcgccgcgtc gagcgtccgc cagaggtact
     6061 cgcccaggat ccccagcgaa aggagctgca ctcccgagaa gaacgcgagc gcgaccatga
     6121 gtgacgtcca gccgcggatc ggctggtcct ggaacacctt cagcaccacg agcaaggccg
     6181 cgtaggcgaa cgcgagaagg gcgagaatcg cgccgaagac cgacatccag cgcaggggcg
     6241 cgtacgaaaa agaaatgacc gagtccagga agagtttgac cttcttcgcg agggtccagc
     6301 gcgacttgcc ctcctgtcgc ggggcgcgtt cgtacgacac gagcgccgtc gggaacccgg
     6361 cccacagaat gagccccggc agagacgtgt gaacctcgcg gccaccgctc aggaagtcga
     6421 tcgccggtcg cccgatcaga aaacagtcga agccgccggc cggcatggat ccgagggcca
     6481 ggcgacggag cgtccggtag tagacggtcg cggccgcccg ggtagcccac ggctccggcc
     6541 gcgccgagcg gaccgcgagg acggccgtcg cgccgccccg ccatgcctcg accagctgca
     6601 ggaggagctc gggaggctcc tgcaggtccg cggaaaggac cgccgccgca tcacccgtc
//
LOCUS       DASWAV010000690         3698 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U2665
            k141_397163, whole genome shotgun sequence.
ACCESSION   DASWAV010000690 DASWAV010000000
VERSION     DASWAV010000690.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960316
            Sequence Read Archive: SRR10260007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 3698)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3698)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:47:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,929
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3698
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_397163"
                     /isolate="SMAG_U2665"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.36444 N 132.31889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1257)
                     /locus_tag="VF958_14580"
     CDS             complement(<1..1257)
                     /locus_tag="VF958_14580"
                     /inference="COORDINATES: protein motif:HMM:NF016289.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family peptidase"
                     /protein_id="HEX9150379.1"
                     /translation="MSRNRMFLAALSLLLVAAAPPTATSRLARQFEKGVRVLASDEME
                     GRGLGTKGIGLAADWIERRLAELKLEPAFAGSYRQPFPVKTGVRRAEGNELSGVSPDD
                     WTPLASSSSGAFDGDLAFVGYGIEAAPLSYQELAPVDLHGKVALMLRYEPQEKDDTSR
                     FDGRRPSRWSAIRYKVHRAREAGAVAVVFVTGPLQDAEQDRLPVLANDGPESAAGIPV
                     LQIRRAVAAKWLAPAGVDLLEFQKAVDADVKPRSLGSVGVRVSGRVALDVTHAQTANV
                     AAKLPGRGALANEAVVIGAHYDHLGWGGAGSMRPNEHAIHNGADDNASGTAAVLAAAE
                     RLKGLVKNARSRRTVYFALFSAEESGIAGSSYFVAHSPVALERVRAMVNLDMVGALRD
                     DQLIAFGAESAPEWTAALESAAKPLGL"
     gene            complement(1310..1750)
                     /locus_tag="VF958_14585"
     CDS             complement(1310..1750)
                     /locus_tag="VF958_14585"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011361920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HEX9150380.1"
                     /translation="MELNIRFPGGLAVEADFDGHTVRTDQPQVSGGGGSAPSPFDLFL
                     ASIGTCAGYYALQFCRARSLPTDGVTLKLTAEKDPVVKRVTRVAIEIDLPPDFPTRYR
                     EAIVRATDQCSVKKHLLDPPEIEVFARPFAAPPGPPEIAPSLPA"
     gene            1881..3119
                     /locus_tag="VF958_14590"
     CDS             1881..3119
                     /locus_tag="VF958_14590"
                     /inference="COORDINATES: protein motif:HMM:NF045502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HEX9150381.1"
                     /translation="MFSPVELEIELFCRGIRIDPTCELGTDGRRITRTRAGLGSGLEL
                     LIPGRRRDHWANVPVVERFVEKSPFRLVRESDAYWIVDDRTGEGYPAALPEEPVWYSR
                     TTSSGVEMSRIGVLQGNYLGIYVSTGCLFWASQPSRACKFCTTGKNVGTVEQARKKVE
                     DVVEVAIAAREESSSVFTHLNTGYHYEEVDRLEAIHGLKQCEPFVRALRRNVGGFIGV
                     QCMPVPSRMFHEYDTLMEAGADHFSFCYEFEDPEVFAAICPGKAQTLGQPAFFEAMEY
                     ASSRLGRGRVSGEIIAGIESIEATKRGIDRIVSAGAFPTVCIFRPTIGSEMEDVAPPE
                     PDDMKEVFAHLYGACRAAQLPIGILPIEVSLVVQPEECADLVEPDFTSRLYGWEMAAL
                     RFLARPYVAWKMRPRSAATA"
     gene            complement(3123..>3698)
                     /locus_tag="VF958_14595"
     CDS             complement(3123..>3698)
                     /locus_tag="VF958_14595"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HEX9150382.1"
                     /translation="GARIFEEGDPADDIYFLFLGRVKIVKMAGDRDLILEILGPGEPV
                     GAVAVYERRPFPATAIALESCGVISIPSREFFSLLEKRPEITRRLLAGLTLRLMALNR
                     RMADMTGSVDHRIGRLLSTLAERTGSKTDDGIFIPLSLSRQDIADLVGTTIETAIRVM
                     SRWHKEGIVETEKNGFRIRDPRALAEISEEG"
BASE COUNT          597 a         1241 c         1261 g          599 t
ORIGIN      
        1 cagcccgagc ggcttcgccg cggattccag cgcggcggtc cactccggtg cggactccgc
       61 gccgaacgcg atcaactggt cgtcgcggag cgctccgacc atgtcgagat tgaccatcgc
      121 gcggacgcgc tcgagggcga cgggggaatg ggcgacgaag tacgaagaac cggcgatccc
      181 cgactcctcg gcggagaaga gggcgaagta gacggtgcgc cggctccggg cgttcttcac
      241 gaggcccttc aagcgctcgg ccgcggcgag cactgcggcg gttccggacg cgttgtcgtc
      301 ggcgccgttg tggatcgcgt gctcgttcgg gcgcatcgag cccgcgcctc cccatccgag
      361 gtggtcgtag tgcgcgccga tgaccaccgc ctcgttcgcc agcgcgcccc ttccggggag
      421 cttcgcggcg acgttggcgg tttgcgcgtg ggtcacgtcg agagcgacgc gtccactcac
      481 ccggacgccc acggagccga gggagcgcgg cttgacgtcg gcatccaccg ccttctgaaa
      541 ttcgaggaga tcgactcccg cgggcgcgag ccatttcgcc gcgaccgcgc gccggatctg
      601 gaggaccggg atgcccgcgg cgctctcggg cccgtcattg gcgagcaccg ggaggcgatc
      661 ctgctccgcg tcctggagag gccccgtgac gaaaacgacc gcgaccgccc cggcctcgcg
      721 ggcgcgatgg accttgtacc ggatggcgga ccaccggctc ggccggcggc cgtcgaatcg
      781 cgacgtgtcg tccttttcct gcggctcgta gcggagcatg agggcgacct tgccgtgcag
      841 gtcgacgggg gcgagctcct ggtacgagag aggcgctgcc tcgattccgt agccgacgaa
      901 ggcgagatcg ccgtcgaaag ccccggagct cgacgaagcg agcggcgtcc aatcgtccgg
      961 cgacactccc gagagctcgt ttccctcggc ccggcggacg ccggtcttca ccgggaatgg
     1021 ctggcgataa cttcccgcga acgccggctc gagcttgagc tccgcgaggc gtctttcgat
     1081 ccagtccgcc gcgagcccga tccctttggt ccccaggccg cgaccttcca tctcgtcgga
     1141 cgcgagaacc cggacacctt tttcgaactg cctggcgagc ctcgaagtcg ccgtcggcgg
     1201 agcggccgcg acgagaagga gcgacagcgc cgccagaaac attctgttcc gggacatggg
     1261 tctcctttcg cccgggggga gcgtacccca gggccgcgag ccgttcgctt caggcgggaa
     1321 gtgaaggggc gatctcgggc ggtcccggcg gcgccgcgaa cggacgcgcg aagacctcga
     1381 tctccggagg atcgagcaga tgcttcttca cgctgcactg gtcggtcgcc cgaacgatcg
     1441 cctcccggta ccgggtcgga aaatccggcg ggaggtcgat ctcgatcgcg acgcgggtca
     1501 cgcgcttcac caccgggtcc ttttccgccg tcagcttcaa cgtcacgccg tcggtcggaa
     1561 ggctgcgcgc ccggcagaac tgcagggcgt agtagcccgc gcacgtgccg atcgaggcga
     1621 gaaagaggtc gaagggggag ggggccgagc cgccgccgcc cgacacctgc ggctgatcgg
     1681 ttcgcacggt atggccgtcg aagtcggcct cgaccgcgag cccgccggga aaacggatgt
     1741 tgagttccat gaacgaatga gacctcggac gacccccgag ggcctgatgc aatcgcatca
     1801 gccgaaaacc cgtcggccga catgatcctg atcatgtcgg ttcacggggg cgcgagccga
     1861 caattggccc gggaggatcc atgttttcac ccgtcgagct cgagatcgaa ctcttctgcc
     1921 gcggaatccg gatcgatccg acgtgcgagc tcggcacgga cgggaggcgc atcactcgca
     1981 cccgcgccgg tctgggatcc ggtctcgaac tgctgatccc cggtcggcgc cgcgaccact
     2041 gggccaacgt gcccgtcgtc gagcgattcg tcgagaagtc gccatttcgc ctcgtccgcg
     2101 agtccgacgc ctactggatc gtcgacgatc ggaccggcga gggatatccc gccgcgctgc
     2161 cggaggagcc cgtgtggtat tcgcgcacca cctcttcggg cgtggagatg agccgcatcg
     2221 gcgtgctcca ggggaactac ctcgggatct acgtctcgac ggggtgtctc ttctgggcgt
     2281 cccagccctc gcgcgcgtgc aagttctgca cgaccgggaa gaacgtcggc acggtcgaac
     2341 aggcgcgcaa gaaggtcgaa gacgtcgtcg aggtcgcgat cgcggcccgc gaggagtcga
     2401 gctcggtctt cacccacctg aacaccggct accactacga ggaggtcgac cggctcgagg
     2461 ccattcacgg tttgaaacag tgcgagccgt ttgttcgcgc cctccggcgg aacgtcggcg
     2521 ggttcatcgg cgttcagtgc atgccggtgc ccagccgcat gttccacgag tacgacacgc
     2581 tcatggaggc cggagccgac cacttctcct tctgctacga gttcgaggac ccagaggtct
     2641 tcgcggcgat ctgtcccggc aaggcgcaga ccctcgggca gccggcgttc ttcgaggcga
     2701 tggaatacgc ctcgtcgcgg ctcggccgcg ggcgcgtctc gggggagatc atcgcgggca
     2761 tcgagtcgat cgaggcgacc aagcgtggaa tcgaccggat cgtctccgcc ggagcgtttc
     2821 cgacggtctg catcttccga ccgacgatcg gaagcgagat ggaggacgtc gctccgccgg
     2881 aacccgacga catgaaagag gtcttcgcgc acctctacgg ggcgtgccgc gccgcgcagc
     2941 ttccgatcgg aatcctcccg atcgaggtgt cgctcgtcgt gcagccggag gaatgcgccg
     3001 acctggtcga gccggacttc acgtcgcggc tgtacggctg ggagatggcc gccctgcgct
     3061 tcctggcccg gccgtacgtc gcctggaaga tgcgaccgcg gagcgccgcc acagcctgac
     3121 gctcagccct cctccgagat ctcggcgagc gcgcgcggat cccggatgcg aaagccgttt
     3181 ttctcggtct cgacgatgcc ttccttgtgc cagcggctca tcacgcggat cgccgtctcg
     3241 atcgtcgttc ccaccaggtc ggcgatgtcc tgacgcgaga gcgagagcgg gatgaagatg
     3301 ccatcgtcgg tcttgcttcc ggtgcgctcc gcaagtgtcg aaagaagccg gccgatccgg
     3361 tggtcgaccg acccggtcat gtccgccatt cgtcggttca atgccatgag acgaagagtc
     3421 agtccggcca gcagcctccg cgtgatctcc ggccgcttct cgaggaggga gaagaactcg
     3481 cggctcggaa tggagatcac cccgcaggat tcgagggcga tcgccgtggc tggaaacggg
     3541 cgtcgctcgt atacggcgac ggctccgacc ggctcgcccg gcccgaggat ctcgaggatg
     3601 aggtcccgat cgcccgccat cttgacgatc ttcacgcgtc cgaggaacag gaaatagatg
     3661 tcgtcggcgg gatctccctc ttcgaagatc ctggcgcc
//
LOCUS       DASWAV010000691         2732 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U2665
            k141_397330, whole genome shotgun sequence.
ACCESSION   DASWAV010000691 DASWAV010000000
VERSION     DASWAV010000691.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960316
            Sequence Read Archive: SRR10260007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2732)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2732)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:47:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,929
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2732
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_397330"
                     /isolate="SMAG_U2665"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.36444 N 132.31889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..322)
                     /locus_tag="VF958_14600"
     CDS             complement(<1..322)
                     /locus_tag="VF958_14600"
                     /inference="COORDINATES: protein motif:HMM:NF014615.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homocysteine S-methyltransferase family protein"
                     /protein_id="HEX9150383.1"
                     /translation="MKKDFRRAFAEEVLLADGAMGTLLVSRGASPDQAKSPLNVSDPE
                     AVREVHEDYCDAGARILTTNTWDANRVKLTAHEWGDSLEKINREGVRLAREAAVGEMV
                     FVAGS"
     gene            753..1157
                     /locus_tag="VF958_14605"
     CDS             753..1157
                     /locus_tag="VF958_14605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9150384.1"
                     /translation="MTILLLLLSLVSPEAASPSSEVALTIERDHGSSSDHVTLCRVRA
                     VNYGGRSWSGRAIAFEARAIRDGAIAATERGRFGLTLLPYGSLETIIGFTGRFDRFEV
                     SRVEGSASGRRSRGGGSRGGGSRGRGKARKSN"
     gene            1169..2455
                     /gene="purD"
                     /locus_tag="VF958_14610"
     CDS             1169..2455
                     /gene="purD"
                     /locus_tag="VF958_14610"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015738353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HEX9150385.1"
                     /translation="MKILVVGGGAREHALCWSLARSSEAEVIYCAPGNPGIAEVADCL
                     PVSSNEIVELADIAEKLRIDLTVVGPEVPLSLGIADEFAKRQLPIFGPSRLASQIESS
                     KAFAKKFFERHGIPTARAVVCSSQDEARSAIGKFGYPVVLKADGLAGGKGVFTVESAA
                     EADKALALFFGERVFGAAGDRVVVEEFLRGQEASFLAICDGEMAVALPTARDYKKVYD
                     GDRGPNTGGMGGHSPAGVLDAEDSSRVLRDVIWPTMRGLAEEGRPFRGVLYAGLMVTD
                     LGPMVLEFNARFGDPETEVILPRVVSDLAVVLRAATARGELEQFSPLEVKAEVCAGVV
                     LCSAGYPGVSVGGKAITGLTEAARQPGVQLFHAHTEREGDRLMAGGGRVIVVTATAPS
                     LSEALGRAYQAAERVEFEGKHFRRDIGAAWLRGKRT"
     gene            complement(2478..>2732)
                     /locus_tag="VF958_14615"
     CDS             complement(2478..>2732)
                     /locus_tag="VF958_14615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9150386.1"
                     /translation="ATNGQRVGGVLTLSNLQPGEVRQLSNIRALAAIPDNVVGMLAFA
                     DVIGASASSPTIEGYVNILDGGTQDGAFFEMKCADTDGCG"
BASE COUNT          404 a          918 c          946 g          464 t
ORIGIN      
        1 tggaaccggc cacgaacacc atctcgccga ccgcggcttc gcgcgcgagc cggacccctt
       61 cgcggttgat cttctcgagc gagtcacccc actcgtgcgc cgtcagcttc acgcggttcg
      121 cgtcccacgt gttcgtcgtc aggatccgcg cgcccgcgtc gcagtagtcc tcgtgaactt
      181 cacgcacggc ctccgggtcg gagacgttca gcggcgactt cgcctggtcc ggagacgcgc
      241 cgcgcgagac cagcagtgtc cccatcgcgc cgtcggcgag aaggacctct tcggcaaaag
      301 ccctgcgaaa atctttcttc atgaacgaaa aacgcctccc aaagtcgaga ggcgtcgtcg
      361 agcgttccgg tcgcggcgcc tctcgcatct ttttcgactc ccgagacggg aaccctgggt
      421 tcccgccgcc gggagaagaa tccggaattg gcacctggcg agccgcgccg cgacgcgcga
      481 atccggttgc cgtgggttag cgtccacctg cgcgaatcgc gttccgccgg gaggacgtcg
      541 ggccggtccc tcacccactc tcgatgtgtc cggcgcgggc gcggcagctg cctgccgtcg
      601 aacacggccg tcgggtcaaa gagccgggcc gaacagtagc cgcgcccggg gaagctgtca
      661 aatccgcgga cacttccggc gggtgcccgc ccgcgctacc ctcacgtcgt cctcctcgag
      721 caggcgttct cccggcccgg aaaggagccg ggatgaccat tcttcttctc ctcttgtctc
      781 tggtttcgcc cgaagcggcg agcccgtcgt cggaggtcgc gctcacgatc gagagggacc
      841 acggttcctc gagcgaccac gtgacgctct gccgcgtccg cgccgtgaac tacggcgggc
      901 gttcgtggtc cgggcgagcg atcgctttcg aggcgagggc catccgcgac ggcgccatcg
      961 ccgcaaccga gcgcgggcgc tttggcctga cgcttcttcc gtacggcagc ctcgagacga
     1021 tcatcgggtt caccggacgg ttcgatcggt tcgaggtctc acgcgtggaa gggtcggcct
     1081 ccggccggcg atcgcgcggg ggcggctccc gcggaggcgg ctcgcgcggc cgggggaagg
     1141 ctcgaaagtc caactagaat gccggtgcat gaagatcctc gtcgtcggag ggggtgcccg
     1201 cgagcacgcg ctttgctggt ctctggcgcg gagctccgag gccgaagtga tctactgcgc
     1261 gcccggaaat cccggcatcg cggaggtcgc cgactgtctc ccggtctcgt cgaacgagat
     1321 cgtcgagctt gccgatatcg cggagaagct gcggatcgac ttgacggtcg tcggccccga
     1381 ggtccccctg tcgctcggaa tcgccgacga gttcgcgaag cgccagctgc cgatcttcgg
     1441 cccgagccgg ctcgcgagtc agatcgagtc ctcgaaagcg ttcgcgaaga agttcttcga
     1501 gcggcacggg atccccaccg ctcgcgccgt cgtctgctcg tcgcaggacg aggcgcgctc
     1561 ggcgatcggg aaattcgggt acccggtcgt cctgaaagcg gatggcctcg cgggagggaa
     1621 gggcgttttc accgtcgaat ccgccgccga agccgacaag gcccttgcgc tctttttcgg
     1681 cgagcgggtt ttcggtgccg ccggcgaccg ggtcgtcgtc gaagagttcc tccgcggaca
     1741 ggaggcgtcc tttctcgcga tctgcgacgg tgagatggcg gtcgcgctgc cgacggcccg
     1801 cgactacaaa aaggtgtacg acggcgaccg gggtccgaac accggcggca tgggaggaca
     1861 ctcgccggcc ggcgttctcg acgcggagga ttcctcccgc gtgctgcggg acgtgatctg
     1921 gccgacgatg cgcggtctcg ccgaagaagg tcgtccgttc cgcggggtcc tctacgcagg
     1981 gttgatggtg accgatctcg gcccgatggt cctcgagttc aacgcgcgtt tcggggaccc
     2041 ggagaccgag gtgatccttc cgcgcgtcgt cagcgacctc gccgtggttc tgcgcgcggc
     2101 gaccgcccgc ggggagctcg agcagttctc acctctcgag gtgaaggccg aggtctgcgc
     2161 cggcgtcgtc ctgtgttcgg cgggctaccc gggcgtgagc gtcggcggca aggcgatcac
     2221 gggcttgacc gaagccgcgc ggcagccggg cgtccagctc ttccacgcgc atacggagcg
     2281 ggagggcgac cgactgatgg ccgggggagg ccgcgtgatc gtcgtgaccg cgacggcgcc
     2341 gtccctgtcc gaagcgctcg ggcgcgccta ccaggcggcc gagcgggtcg aattcgaggg
     2401 aaagcatttc cgccgcgaca tcggagcggc gtggctgcgc gggaaacgga cctagtcgcg
     2461 gcgcgcgcct ctcgtcttta gccgcagccg tcggtatcgg cgcacttcat ctcgaaaaag
     2521 gcgccgtcct gcgtgcctcc gtccaggatg ttcacgtaac cttcgatcgt ggggctggac
     2581 gcagaagctc cgatcacgtc tgcgaatgcg agcatcccga cgacattgtc ggggatcgcc
     2641 gccagtgcgc ggatgttgct cagctgacgg acctctccgg gctggaggtt cgagagcgtg
     2701 agaacgcctc cgactcgctg tccgttggtc gc
//
LOCUS       DASWAV010000692         4337 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U2665
            k141_598025, whole genome shotgun sequence.
ACCESSION   DASWAV010000692 DASWAV010000000
VERSION     DASWAV010000692.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960316
            Sequence Read Archive: SRR10260007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 4337)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4337)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:47:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,929
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4337
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_598025"
                     /isolate="SMAG_U2665"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.36444 N 132.31889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..303)
                     /locus_tag="VF958_14620"
     CDS             complement(<1..303)
                     /locus_tag="VF958_14620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013925339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretory system conjugative DNA
                     transfer family protein"
                     /protein_id="HEX9150387.1"
                     /translation="MSDLYLGRTTDSKPLLYDSSDLVTHGVCVGMTGSGKTGLCIDLL
                     EEALLGDVPLFLIDPKGDVTNLFLLFPELAPEDFARWVDPDAARRAGRTPAEEGAAQ"
     gene            complement(422..1033)
                     /gene="clpP"
                     /locus_tag="VF958_14625"
     CDS             complement(422..1033)
                     /gene="clpP"
                     /locus_tag="VF958_14625"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019397797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009368 - endopeptidase Clp complex
                     [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HEX9150388.1"
                     /translation="MVLVPMVIEQTSRGERAYDIYSRLLKDNVIFLGTPIDDQIANLI
                     IAQMLFLESENPDKDISFYINSPGGVTSAGFAIYDTMQYVKPDVSTICIGQAASMAAV
                     LMAAGTPGKRFALPNSRFLIHQPWTTGLQGQTADIEIHAKDMLKLRDQINKVLAQHTG
                     QSIEKIAKDTDRDYILEAEDARKYGLVDAIYHRRDDVALKAAG"
     gene            complement(1101..2414)
                     /gene="tig"
                     /locus_tag="VF958_14630"
     CDS             complement(1101..2414)
                     /gene="tig"
                     /locus_tag="VF958_14630"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR00115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005854 - nascent polypeptide-associated
                     complex [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0051083 - 'de novo' cotranslational protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HEX9150389.1"
                     /translation="MVLLSSRDESQTRAVLDIEVPAEDVERTYGAVARAFARQAALPG
                     FRRGHVPESLVQQRFAGEIREQVLERLLPAALSSAVEEKNLSVLGHPHVENLEWNPPG
                     PIRFSARLDLKPAVDPGEYRGIPVEDVSVEPSEEELSQVIDRIRESHAEFHPIEGRAA
                     AAGDFAVADIAGRFIEILEPGQNPRTFRDEKLTLEVGHPDSMAEINEALMGALPGEAR
                     SFRKSFADDFVNEEFRGKTVDYEVTLVALKEKRLPAFDDDFARAVAQTESAETLREKV
                     RANLRQEKESARRRKFRRDILETILSRASVPAPEVLVESEAASSLRDYARYLAANGMD
                     PKEADWKKIREDARPGAERRVREYLVLDAVARREDISVTDTELDAEVKRAAVRRGVEA
                     SALREQLAKNDGLEALRDEMRLSRALDLLISSARVLPSMEPVEPG"
     gene            2616..>4337
                     /locus_tag="VF958_14635"
     CDS             2616..>4337
                     /locus_tag="VF958_14635"
                     /inference="COORDINATES: protein motif:HMM:NF024816.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX9150390.1"
                     /translation="MLATAVVLVWIGVLRNGFVTYDDELYVTRNPHVLSGLTTSGIGW
                     AFSTSYGGNWHPITWLSHMMDMELFGAEPRGHHATSLLLHVANTLLLYRLIQRMSGAR
                     LRSAIAAVLFAVHPLRVESVAWISERKDLLCAFFGLLSILCYVEYVPDRNRPRYAMSI
                     VLFALSLASKPMLVTLPFLLLLLDWWPLKRLNHSNARDLVIEKLPYLALSAASSVVTY
                     FVQRTAGATTALTVPIAVRLDNAILSYVRYMGKLLWPVRLAALYPYSLITHGWMTAAA
                     AMLLLSLTAACLRFGRRYPLLPVGWLSFIGTLVPVIGLVQVGWQSMADRYTYLPTIGL
                     ILIIVWGIADLGPRSAPGRVTGASASAAAFVVLSVLTFRQIGTWRDSITLFQHALSVT
                     DRNDTMEINLGNALLREGRLDEARLHLERAVDICRTCPDARFELGVVHATAGTLDQAV
                     AQFEEALRLKPDLAEAHERLGFVLFSQRRWDEAVRHLEQALRLRPDSPKTLYALGAAL
                     EGGGHFAEAIGRYEAALHLDPNSAHAHSNLGLLLLNERKAAEALPHLRATVRLDAGSS
                     QAHSDLAQ"
BASE COUNT          701 a         1419 c         1370 g          847 t
ORIGIN      
        1 ctgcgccgcc ccttcttccg ccggcgttcg gcccgcacgg cgtgccgcgt cggggtcgac
       61 ccagcgggcg aaatcttccg gcgcgagctc cgggaagagg agaaagaggt tcgtcacgtc
      121 gcccttcggg tcgatcagga agagcggaac gtcgccgaga agcgcctctt cgaggagatc
      181 gatgcagaga cccgtcttgc cgctgcccgt catcccgacg cagacgccgt gcgtgacgag
      241 gtcgctgctg tcgtagagga ggggcttgga atccgtcgtt cggccgagat agagatcgct
      301 catgaggccc ggagtttatc gtcaggtcag tccgcggtcc tcggtcctcg gtgctcggtc
      361 gaagatctcg cgttcgaccc gggagagagg atttgcaact gagaacttga aagcggtggc
      421 tttagcccgc cgccttcagc gcaacgtcgt ctcggcggtg gtagatggcg tcgacgaggc
      481 cgtacttgcg ggcgtcctcg gcctccagga tgtagtcccg gtcggtgtcc ttcgcgatct
      541 tttcgatcga ctgcccggtg tgctgcgcaa ggaccttgtt gatctggtcc cgcagcttca
      601 gcatgtcctt ggcgtggatc tcgatgtccg cggtctgtcc ctggaggccg gtcgtccacg
      661 gctggtgaat caggaaccgc gagttcggca gcgcgaaccg cttccccgga gtgccggcgg
      721 ccatgagcac cgccgccatc gaggcggcct gtccgatgca gatggtcgag acgtcgggct
      781 tgacgtactg catggtgtcg tagatggcga agccggcgga agtcactccg cccggcgagt
      841 tgatgtagaa cgagatgtct ttgtccgggt tctcggactc gaggaagagc atctgcgcga
      901 tgatcaggtt cgcgatctgg tcatcgatcg gcgtgccgag aaagatgacg ttgtctttga
      961 gcagccgcga atagatgtcg taggcccgct ctccccggct cgtctgctcg atcaccatcg
     1021 gtacgagaac cattccgccc ccctcgcctt cttcgttgtt caattcggtc aaccctccgc
     1081 cggtcgatgc cattcctgga ctagcccggc tcgaccggct ccatcgaggg taacacccgc
     1141 gcggaggaga tcaagaggtc gagcgcccgc gacagccgca tctcgtcgcg cagggcttcc
     1201 aggccgtcgt tcttcgcgag ctgctcgcgc agcgcggaag cttcgacgcc gcggcggacc
     1261 gcggcgcgct tgacttcggc gtcgagctcg gtgtcagtga ccgaaatgtc ctcgcgacgc
     1321 gccaccgcgt cgaggacgag gtactcgcgc acgcgacgct cggctcccgg acgggcgtcc
     1381 tcgcggatct tcttccagtc cgcctccttc ggatccatgc cgttcgccgc caggtaacgg
     1441 gcgtagtcgc gcagcgacga ggcggcctcc gattcgacga gcacctccgg cgccggcacg
     1501 ctcgccctcg agaggatcgt ctcgaggatg tcgcgccgga acttccggcg ccgcgcggac
     1561 tctttctcct ggcggaggtt cgcgcggacc ttctcgcgaa gcgtctcggc cgactcggtc
     1621 tgtgcgaccg cgcgcgcgaa gtcgtcgtcg aaagccggaa ggcgcttctc cttcagcgcg
     1681 acgagcgtca cctcgtagtc gacggtcttc ccccggaact cctcgttcac gaagtcgtcg
     1741 gcgaagctct tgcggaacga acgcgcctcg cccggcagtg ctcccatcaa cgcttcgttg
     1801 atctccgcca tcgaatcggg atggccgacc tcgagcgtca gcttctcgtc gcgaaaagtc
     1861 ctcgggttct gtcccggctc gaggatctcg atgaagcgcc cggcgatgtc ggccacggcg
     1921 aaatcgcccg ccgccgccgc gcgcccctcg atcggatgga actcggcgtg agactcgcgg
     1981 atgcggtcga tgacctgcga caactcctcc tcggaaggct cgaccgagac gtcctcgacc
     2041 ggaatgcccc ggtactcccc cggatcgacg gccggtttga gatcgagacg agccgagaag
     2101 cggatcggtc cgggcggatt ccactcgagg ttctcgacgt gcgggtggcc gaggaccgaa
     2161 aggttcttct cctcgacggc cgaggagagc gccgccggca acagccgctc gagcacctgc
     2221 tcccggatct cgccggcgaa acgctgctgg acgagggatt ctggaacatg gccgcgccgg
     2281 aaaccgggca gagccgcctg gcgcgcgaag gcgcgagcga ccgcgccgta agtgcgctcg
     2341 acgtcctcgg ccgggacctc gatgtcgagg acggcgcgtg tctgcgattc atcgcgtgac
     2401 gaaagaagga ccatggcgga gttgagtcta ctggacgaag cgatcggctg ttagccgccg
     2461 tctccggcga gactcactgc ttctggagta gcctgaccgg agtgcgttcg aaaaggtccc
     2521 gccaccaccg gcgtgaggtc gaacggcggt cgaggcaatt ccgaagagat acggtcacac
     2581 aggagtggcg tggcggcgag gccggagtct acgcgttgct tgcaaccgcc gtggtcctcg
     2641 tctggatcgg ggttctgcga aacggcttcg taacctacga cgatgaactc tacgtgacgc
     2701 gaaatccgca cgttctctca gggctgacga cctcgggaat cggatgggcc ttctcgacca
     2761 gctatggcgg aaactggcat cccatcacct ggctctcgca catgatggac atggagctct
     2821 tcggcgccga accgcgagga caccatgcga cgagtctcct gcttcatgtc gcgaacaccc
     2881 ttctcctgta ccgcctgatc cagcgcatgt cgggcgcacg attgcgcagc gcaatcgctg
     2941 cggttctctt cgctgttcat cctttgaggg tcgagtcggt ggcgtggatc tccgaacgga
     3001 aagatctcct ttgtgccttt ttcggcctcc tgtcgatcct gtgctacgtc gagtacgtcc
     3061 ctgacaggaa caggcctcgc tacgcgatga gcatcgtcct cttcgcgctt tcgctcgcgt
     3121 cgaagccgat gctcgtgacg ttgccatttc tgcttctcct gctcgactgg tggccactga
     3181 agcgcctgaa ccactcgaac gccagggatc tcgttatcga gaagctcccg tacctcgcgc
     3241 tttccgcagc atcgagtgtc gtgacctatt tcgtgcagcg aaccgccgga gcgaccaccg
     3301 cgctcacagt tccaattgcg gttcgcctcg acaacgccat cctctcttac gtgcgctaca
     3361 tgggcaaact gttgtggccg gtccgcctgg ctgccctgta tccctattcg ctcatcacgc
     3421 acggatggat gacggcggca gccgcaatgc ttcttctctc gttgactgcc gcgtgcctgc
     3481 ggtttggaag gcgctatccc ctccttccgg tcggttggct ttcgtttatc ggcacgctcg
     3541 tccctgtcat cgggctcgtg caggtgggct ggcaatcgat ggcggaccgc tatacgtacc
     3601 tcccgacgat cggcctgatc ctgatcatcg tctggggaat cgcagatctc ggcccgcgat
     3661 cggccccggg gcgcgtgacg ggcgcctccg cgtcggcagc tgcgttcgtc gtgctctccg
     3721 tgctcacttt ccgccagatc ggtacctggc gagattcgat caccctcttc cagcatgccc
     3781 tctcggtcac cgatcggaac gacacgatgg agatcaatct cggcaacgca ttgctccgcg
     3841 aggggcgact cgatgaggcg cgactacatc tcgagcgtgc cgtcgacatc tgtcgcacgt
     3901 gcccggacgc ccgattcgag ctgggcgtcg tgcacgccac ggccggcact ctcgaccagg
     3961 cggtcgcaca gttcgaggaa gcgcttcgct tgaagccgga tctggccgag gcccatgagc
     4021 gactgggttt cgtgctgttt tcgcaaaggc ggtgggacga ggccgttcgg caccttgagc
     4081 aagcccttcg gttgcggccc gattcgccga agactctcta cgcgctcggt gccgccctcg
     4141 agggaggagg ccatttcgcc gaagcgatcg ggcgttatga agcagctctg catctcgacc
     4201 cgaactccgc tcatgctcac agcaatctcg gattgctgct gctcaacgaa agaaaggccg
     4261 cagaggcctt gccacacctc cgagcgacgg tacggctcga tgccggatct tcgcaggcac
     4321 attccgacct cgcacag
//
LOCUS       DASWAV010000693         4558 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U2665
            k141_598177, whole genome shotgun sequence.
ACCESSION   DASWAV010000693 DASWAV010000000
VERSION     DASWAV010000693.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960316
            Sequence Read Archive: SRR10260007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 4558)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4558)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:47:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,929
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4558
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_598177"
                     /isolate="SMAG_U2665"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.36444 N 132.31889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(102..1511)
                     /locus_tag="VF958_14640"
     CDS             complement(102..1511)
                     /locus_tag="VF958_14640"
                     /inference="COORDINATES: protein motif:HMM:NF019850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HEX9150391.1"
                     /translation="MPDSRRIHLVPIGGTAMTPLAALFVEEGHRVTGSALDLYPPMST
                     LLESLQIPVAKGFAPEHVAADCDCVVVGNAALRDNVEAAEAVRRGIPVLSMPQAIRQH
                     LLPGKTSVVITGTHGKTTTSALTAWLLLDSGRDPGFLIGGEMKNLGRGYRRGAGPAVV
                     LEGDEYNAAFFDRGPKFLHYEPKHLFVGNIEYDHADLFPDLAAVVDAFRQVVAIVPPD
                     GTVVANVDDERVRGILPAARQAAARIVRVSLDDPSADFSATEIEAHPDGTRFTLLESG
                     RPAGRLSSPLLGTHNLRNALGATALVRGLGMSVDEIARSLPRFAGVKRRLEVKGEKNG
                     IVVVDDFAHHPTAVEGTIAAARTRWPGRRLWVLFEPRSNTAGRKLFEDDYAAAFANAD
                     ALVIGPVFHAKRLKPEERIDRAAVLSRFTNNGKPAYAPESLDEIPAILRREARPGDVL
                     LLMSSGAFGGLPETLLGEL"
     gene            1639..2688
                     /locus_tag="VF958_14645"
     CDS             1639..2688
                     /locus_tag="VF958_14645"
                     /inference="COORDINATES: protein motif:HMM:NF019242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1573 domain-containing protein"
                     /protein_id="HEX9150392.1"
                     /translation="MRQFGRLTGLAMAGLLIVAGLEAQAAKAKPPAGKPPVAAPKMEI
                     SPETKDAGTVAKGQLVDAVFVVKNTGGSDLIISDARPSCGCTVASFDKVIKPGAEGKI
                     QSSVDTKSFSGPISKSVLVVSNDPDRPQVNLFIKALVKPYVDVLPQAFVRFSVIKGDN
                     AGQDVILLSDEKGFRPTIAETSQPYVKAEILPAGDKDKIAGRSGDQYRVRINVTPDAP
                     EGLLNAPIRVATGVSQQPTIEIPVSGIVRPRVSVTPVTVNFGNFTAGKDPITRNIIVT
                     NNKPAMPVKVTRAEVSVAGFITDIVPTQEGVSYTVVVKASGAVKKGALDGTVKLFTSD
                     KERAVIEIPLRGEVL"
     gene            2814..4361
                     /locus_tag="VF958_14650"
     CDS             2814..4361
                     /locus_tag="VF958_14650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9150393.1"
                     /translation="MPSHGGARPPERGKVGWGLFPSLAALLLLHSTGAAASLDPDLVS
                     KFSIVLPAAASLHGLPPAFFHSDVAVLNTSIRRIAEVTAVYRCNGGSCGNALRTFNVA
                     PRQVLLLEDVVGNFFGAPETSGAIEFSSTERIVVTSHLYTPARPSPTFGMLVPGLTPA
                     EALVDVELVNLSHSADPSVGYRTNLAIYNPTDQTLSAFIGCRDFRSAALGFMTQDVGP
                     HQLLQINDGDLFREFGVLRNVSSFNCSIRGGFLTPFYSWASVLDNVSNDLTFVRAQQF
                     FAVPRPHTIPAVASLHGVGGTFFRSDVAIFNDDVLAGADVIATYRCLQGSCPVSDRFL
                     SLGPGETRILADAVGSFFAAPESSGSIEFVTSGHISVTSRLYAPASGGGTVGTSVPAV
                     DSRAATPSRVLVLMPRGSRVNVGVVNFEDVAQRVRIRLFSAAGEFLGDIARVVDRRQS
                     AQVNDVFGELGITADLAAAYCLVEGEGSLRILAYAAIIDNESQDSIFVSGEDDPDTPR
                     VNLRSTP"
     gene            complement(4374..>4558)
                     /locus_tag="VF958_14655"
     CDS             complement(4374..>4558)
                     /locus_tag="VF958_14655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9150394.1"
                     /translation="SEATSWGKVSKKASHEMAFIETSVALPLLYGFALQKQAHKGRKR
                     LRYEWKDDILERVTRR"
BASE COUNT          763 a         1540 c         1461 g          794 t
ORIGIN      
        1 gcggattcca gagtccgcga ggatcaccgg cggtcgggga atctgaaaca ggaaatcgaa
       61 ccccaggagg agcagggctg ccagcggcac gatgcgctcc cctagagttc tccgagcagc
      121 gtttccggca ggccgccgaa cgcgcccgac gacatgagca gcaacacgtc gccggggcgc
      181 gcttcgcggc gcaggatcgc cgggatctcg tccaggctct cgggcgcgta ggcgggcttc
      241 ccgttgtttg tgaagcggga gagcaccgcc gcgcggtcga tccgctcctc gggcttcagg
      301 cgcttcgcgt ggaaaaccgg cccgatgacg agcgcgtcgg cgttcgcgaa cgcggccgcg
      361 tagtcgtcct cgaacaactt tcttccggcg gtgttcgagc gcggctcgaa gagaacccag
      421 aggcgccggc ccggccagcg tgttcgcgcg gcggcgatcg ttccctcgac cgccgtcggg
      481 tggtgcgcga agtcgtccac gacgacgatc ccgttcttct cccccttcac ctcgagccgg
      541 cgcttcacgc cggcgaagcg cgggagcgag cgtgcaatct cgtcgaccga catgccgagc
      601 ccgcgcacga gcgcggtcgc gccgagcgcg ttgcgcaggt tgtgcgtgcc gaggagcggt
      661 gaggaaagcc gccccgcagg gcgacccgac tcgaggagcg tgaagcgcgt cccgtcggga
      721 tgcgcctcga tctccgtcgc cgagaagtcc gccgacgggt cgtcgagcga aacccgcacg
      781 atccgggcag ctgcttgtcg cgccgccggg aggatcccgc gaacccgctc gtcgtctacg
      841 ttggcgacga cggtcccgtc gggcggaacg atcgcgacga cctgtcggaa cgcgtcgacg
      901 accgccgcga gatcgggaaa caggtccgcg tggtcgtact cgatgttccc gacgaagagg
      961 tgcttcggct cgtagtggag aaacttcggc cctcggtcga agaacgcggc gttgtactcg
     1021 tccccctcga gaacgacggc gggaccggcg ccgcgccggt acccgcggcc gaggttcttc
     1081 atctccccgc cgatcaggaa cccggggtcg cgtcccgagt ccagcagcag ccacgcggtc
     1141 agcgcggacg tcgtcgtctt gccgtgcgtg ccggtgatga cgacggaggt ctttcccgga
     1201 aggaggtgct gacgaattgc ctgtggcatc gagaggacag ggatccctcg ccggacggcc
     1261 tcggccgcct cgacgttgtc gcgcaacgcc gcgttcccga cgacgacgca gtcgcagtcg
     1321 gcggcaacgt gctccggcgc aaagcccttc gcgaccggga tttggaggga ctcgaggagg
     1381 gtggacatcg gcgggtacag gtcgagggca cttccggtca cgcgatggcc ctcttcgacg
     1441 aacagcgcgg ccagcggcgt catcgccgtt ccgccgatcg ggacgaggtg aatcctgcgg
     1501 gaatccggca tgaacccttc ggaaatggac gggaatggcc ggcaaacccg cccgattctt
     1561 gaaataaccg ccgcttgtcc ggagtttgac cggttcggca atactctaaa gcacaagcgc
     1621 caaacaggag gatcggaaat gcgtcagttt ggaaggttga ccggccttgc catggcgggc
     1681 ctgctcatcg tggccgggct cgaagcgcag gccgcgaaag ccaagccgcc cgcgggtaag
     1741 ccgcccgtcg ccgcaccgaa gatggaaatc tcgcccgaga cgaaggacgc cggcacggtc
     1801 gccaaaggcc agctggtcga tgcggtcttc gtcgtgaaga acaccggagg ctccgacctc
     1861 atcatttccg acgcacgccc ctcctgcggt tgcacggtgg cctcgttcga caaggtcatc
     1921 aagccgggcg ccgagggcaa gatccagtcg agcgtggaca cgaagagctt ctccggaccg
     1981 atcagcaagt ctgtgctcgt cgtctcgaac gacccggacc gtccgcaggt aaacctgttc
     2041 atcaaggccc tggtgaagcc gtacgtcgac gtgttgccgc aggcgttcgt ccggttctcc
     2101 gtcatcaagg gggacaacgc tggccaggac gtgatcctcc tctccgacga gaagggcttc
     2161 cggccgacga tcgccgagac ctcgcagccc tacgtcaagg cggagatcct ccccgccggc
     2221 gacaaggaca agatcgcggg gcggagcggc gaccagtaca gggtccgcat caacgtgacg
     2281 ccggacgcgc ccgagggtct gctcaacgcg ccgatccgcg tcgcgacggg cgtgtcgcag
     2341 cagccgacga tcgagatccc ggtttcgggg atcgtgcggc cgcgcgtctc ggtcacgccg
     2401 gtgaccgtca acttcggcaa cttcaccgcg ggcaaggacc cgatcacccg caacatcatc
     2461 gtgacgaaca acaagccggc gatgccggtg aaggtcacgc gggcggaagt gagtgtcgcc
     2521 gggttcatca ccgacatcgt gccgacccag gaaggcgtct cctacaccgt ggtcgtcaag
     2581 gcgagcggcg ccgtgaagaa gggcgcgctc gacgggacgg tgaagctctt cacctcggac
     2641 aaggagcgcg cggtgatcga gatcccgctg cgcggagaag tgctctaggt tcttctctca
     2701 ccagttcgga aactcgacgc cccgcgaaag cggggcgttt tgtttggagc cgacgaagcc
     2761 tgatccccct gcactagatg gttccccgaa ggcgggcgta tacccatcag cgcatgcctt
     2821 cgcacggggg agcccggccg ccggaaaggg gaaaggttgg ctggggactg ttcccgtctc
     2881 tcgccgcgct gcttctcctt cactcgaccg gcgcggccgc ctctctcgac cctgatctcg
     2941 tttcgaagtt ctcgattgtc ctcccggcgg cggcttcgct ccatggactc ccgcccgcgt
     3001 tctttcattc cgacgtcgcc gtgctcaaca cctcgattcg caggatcgcg gaagtgaccg
     3061 cggtctaccg ctgtaacggc ggctcctgcg gaaacgcttt acggacgttc aacgtcgcgc
     3121 cgcggcaggt tcttctgctg gaggacgtcg tggggaactt tttcggtgct ccggaaacga
     3181 gcggggcaat cgagttctcc agcacggaac gcatcgtggt gacgagccat ctctacactc
     3241 cggcgcgccc gtcgccgacg ttcgggatgc tcgttccggg actcaccccg gcggaggcgc
     3301 tcgtggacgt cgagctcgtg aacctttccc actcggccga tccgtccgtc ggataccgga
     3361 cgaacctcgc gatctacaac ccgaccgacc agaccctatc cgccttcatc ggctgtcggg
     3421 atttccgctc cgccgctctc ggattcatga cgcaggacgt cggcccacat cagctccttc
     3481 agatcaacga cggcgatctc tttcgggagt tcggcgtctt gaggaacgtt tccagcttca
     3541 actgctcgat tcgaggcggc ttcctgacgc ctttctactc gtgggcttcg gttctcgaca
     3601 acgtgtcgaa cgatctgacc ttcgtcagag cccagcaatt cttcgcggtc ccgagacccc
     3661 acacgatccc ggccgtggct tcgcttcacg gggtgggagg aacctttttc cgatccgacg
     3721 tcgcgatctt caacgacgac gtcctcgcgg gcgcagacgt gatcgccacc tatcgatgtc
     3781 tgcagggcag ctgccccgtg tcggaccgat tcctgagcct cgggccgggc gagacgcgaa
     3841 ttctcgcgga cgccgtcgga agtttcttcg cggcacccga gtcctccggg tcgatcgagt
     3901 tcgtgacctc cggtcacatc tccgtcacga gtcgtctcta cgcgcccgct tcgggtggcg
     3961 gcacggtcgg cacgtccgtg cccgcggtcg attcccgcgc ggcaacgccc tcccgggttc
     4021 ttgttctgat gccccgcggt tcccgcgtca atgtcggcgt cgtcaacttc gaggacgtgg
     4081 cgcaacgggt caggatccgt cttttttctg cggcggggga attcctcgga gacatcgcac
     4141 gcgtggtcga ccggcgacaa agcgcccagg tgaacgacgt cttcggcgag ctcggtatca
     4201 cggccgatct cgccgccgcg tattgcctcg tcgaaggaga aggatcgctt cgaatcctcg
     4261 cctatgccgc gatcatcgac aacgagtccc aggactcgat cttcgtgtcg ggagaggacg
     4321 atcccgacac gccccgggtc aaccttcggt caactccgta gacctggccc tcttcaccgc
     4381 cgcgtcaccc tttccaggat gtcgtccttc cactcgtacc gcagtcgctt ccgtcccttg
     4441 tgcgcctgct tctgcaacgc gaacccgtac aggagcggca gcgccaccga cgtctcgatg
     4501 aacgccatct cgtgcgacgc cttcttcgac accttgcccc acgacgtcgc ctcggaca
//
LOCUS       DASWAV010000694         5324 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U2665
            k141_598627, whole genome shotgun sequence.
ACCESSION   DASWAV010000694 DASWAV010000000
VERSION     DASWAV010000694.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960316
            Sequence Read Archive: SRR10260007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 5324)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5324)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:47:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,929
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5324
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_598627"
                     /isolate="SMAG_U2665"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.36444 N 132.31889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..220
                     /locus_tag="VF958_14660"
     CDS             <1..220
                     /locus_tag="VF958_14660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9150395.1"
                     /translation="FYTVNYSTGDPGPGALGSDRGETQQHASFLTNPVFYNESSVIYT
                     TDNTVEKKEVAGVRRTTVKDWKENERPK"
     gene            complement(231..887)
                     /locus_tag="VF958_14665"
     CDS             complement(231..887)
                     /locus_tag="VF958_14665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012234369.1"
                     /note="This protein belongs to the uracil DNA glycosylase
                     superfamily, members of which act in excision repair of
                     DNA. However, it belongs more specifically to UdgX branch,
                     whose founding member was found to bind uracil in DNA
                     (where it does not belong), without cleaving it, appears
                     to promote DNA repair by a pathway involving RecA, rather
                     than base excision; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UdgX family uracil-DNA binding protein"
                     /protein_id="HEX9150396.1"
                     /translation="MLPNSVVPKRTRSDETAAPLVPPRPTLPKLRAAAARCRACDLWE
                     RGTQTVFGEGSSRARVMFVGEQPGDSEDIEGRPFVGPAGRILDSSLEKAGIDRSEVYV
                     TNVVKHFKWEPRGKRRIHQKPNSVEISACRPWLDAELAVVRPDVLVCLGATAAQALLG
                     RAFKVSRQRGHFVVSPLAPKVLATVHPSSILRAPDDETRRAETVKFVEDLKKVARALR
                     "
     gene            936..1820
                     /locus_tag="VF958_14670"
     CDS             936..1820
                     /locus_tag="VF958_14670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011683226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX9150397.1"
                     /translation="MDLVVGATGVLGGEICSRLARAGGSVRALVRKTSNAERVEALKS
                     LGVELAWGDLTDRASLDAACRGVHTVFSTATAIQSQQEGNTLDRVDREGQANLVDAAR
                     GAGVRRFVFISFRMGRDDVDFPLRAAKRSTAERLKASGVPWTVIEASIFMEVWLGPHL
                     GFDAANGRVRVLGSGDQKISWVSYRDVAAIAIAAAESPDAENRTIAVGGPEALSPNEV
                     VRIFEERSGRKFEVERVPESAIAQMRANARNPVEETFAGLMLFCARGDEIDMRQTLSR
                     YPIRMTTVRDYADRVLAG"
     gene            1930..2526
                     /locus_tag="VF958_14675"
     CDS             1930..2526
                     /locus_tag="VF958_14675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9150398.1"
                     /translation="MPAAVRRAEHPGTAIVWQQEIFEVVAAEPLAEGAIRYSLEPWRN
                     DFAIRSLERYDGESESARATERRWRAEAPRRRRLAVLFSPLLGHLPGSVQEKMEKDFG
                     APANAMTVVSAFPLFVVGAFGLFAFIARLAGGSLTPLPEPSLPLSLYLVGESSLRLVI
                     VVSQSRPVGSIPGILLYGIGSRIVARLRGTAVASGGRP"
     gene            2523..2882
                     /locus_tag="VF958_14680"
     CDS             2523..2882
                     /locus_tag="VF958_14680"
                     /inference="COORDINATES: protein motif:HMM:NF024864.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupredoxin domain-containing protein"
                     /protein_id="HEX9150399.1"
                     /translation="MKSTPTRFLLFMVLVGIAPVLLAAPGEARKIEVVAHKYAFDPSK
                     IEIKAGETVEITFRSTDTKHGFVCKELGLEKVVFTKEKPATVTFTPDKPGTYPFKCAR
                     FCGLGHPKMKGQIVVTP"
     gene            complement(2938..4479)
                     /locus_tag="VF958_14685"
     CDS             complement(2938..4479)
                     /locus_tag="VF958_14685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020648138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide MFS transporter"
                     /protein_id="HEX9150400.1"
                     /translation="MTQANPADKKWFGHPRGLSTLFFTEMWERFSYYGMRGILLLFLV
                     ATIQNGGFGLTDRTGAAIYGMYVGLVYLMALPGGWVADRILGQRRAVFIGGCFIAAGH
                     FSMAIGSVATFYLGLCLIVIGTGLLKPNVSAMVADLYPEGGNRRDAGFSIFYSGINVG
                     AMLGPLVCGFLGEKVNWHLGFGAAGVGMVLGLVQYRLGGKYLGEAGLRRSSEVTSASD
                     RSAVRKLALAAAAVLGAIVLAVLLQLAGIVHLDLYGVAKGVGFFMLGLALVYFGAIIF
                     LSRLSRAEKKRIAVIFVFFMAAVLFWAGFEQAGSSMNLFGERMTQRVILGWEMPASFL
                     QAVNSIFIILLAPVFGALWVRLGRKQPSTPAKMGFGLAFLAIGFLVLAWGASFTAGGA
                     RVSMMWLILTYLLHTVGELCLSPVGLSSVTKLSPRSLVGQMMGTWFMGTALGNLIAGL
                     AAGGFEGMGVEELFLSVAKVTGVAGLVLLVLAKPIRKLMGVLPEDDIAPVVEPKATSP
                     AVSPS"
     gene            complement(4548..>5324)
                     /locus_tag="VF958_14690"
     CDS             complement(4548..>5324)
                     /locus_tag="VF958_14690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013217925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEX9150401.1"
                     /translation="LTTVVPYNDGDALEEAFRRHRGEIAAVVLEPVAANMGVVPPRPG
                     YLERARELTFKHGALLVFDEVITGFRVARGGAQELYGVSADLTILGKILGGGLPVGGY
                     GGRRDLMAQVAPSGGVYQAGTLSGNPLAMAAGIAMLREIDRRSPYAELERKGALLESE
                     VSKAIREAGAGDCVTYQRVGSLSTLFFTGGPVTDFTSARRSDTARYGAFFHAMRERGV
                     FLPPAQFEAWFVSTAHSDADLRRTARAVGESLTETATGNR"
BASE COUNT          950 a         1765 c         1772 g          837 t
ORIGIN      
        1 tttctacacg gtcaattact cgacgggaga tccgggcccc ggggcgctcg gatccgatcg
       61 aggggagacc cagcagcatg cgagcttcct cacgaacccc gtcttctaca acgagtcgag
      121 cgtcatctac acgacggaca ataccgtcga gaagaaggaa gtcgcgggcg tgagacgcac
      181 gaccgtgaag gactggaagg agaacgagag accgaagtag cgggcgccgc tcagcggagc
      241 gctctcgcca cttttttcag atcctcgacg aactttaccg tctcggctcg ccgggtctcg
      301 tcgtccggcg cgcgcaatat cgaggacggg tgcacggtcg cgagcacttt gggcgcgagc
      361 ggagagacga cgaagtggcc gcgctgccgg ctgaccttga aggcccgtcc gaggagcgcc
      421 tgagccgcag tcgcgccgag gcacaccagg acgtcgggcc ggacgaccgc gagttccgcg
      481 tcgagccacg gccggcatgc ggagatctcg acgctattcg gcttctggtg gatgcgacgt
      541 tttcccctcg gctcccactt gaagtgcttg accacgttgg tgacgtagac ctcgctgcga
      601 tcgattcccg ccttctcgag tgaggagtcc aggattcgcc cggcgggccc gacgaacggc
      661 cgcccctcga tgtcttcgct gtccccgggc tgctcgccga cgaacatgac gcgcgcccgc
      721 gacgacccct ctccgaagac agtctgcgta ccccgctccc agaggtcgca ggcccggcat
      781 cgggcagcgg cggcacgaag cttcggcagc gtggggcggg gcggcacgag gggcgccgcg
      841 gtctcgtcgg agcgcgtccg cttcggcacg accgagttcg gaagcattcc gcacgccagt
      901 tcctcctgat agcctctccg cgaaaagggg gacagatgga cctcgtcgtc ggagccaccg
      961 gagtcctcgg aggagagatc tgttcgcgcc tggccagggc gggcgggtcg gtgcgcgccc
     1021 tcgttcggaa gacgtcgaac gcggaacggg tcgaggccct gaagagcctg ggggtcgagc
     1081 tcgcgtgggg ggatctgacg gatcgggcat cgctcgacgc ggcgtgtcgc ggcgtacaca
     1141 cggtcttttc gacggcgacg gcgatccagt cgcagcagga gggaaacacg ctcgaccgcg
     1201 tggaccgcga gggtcaggcg aacctcgtcg acgcggccag gggcgcgggg gtccggcgct
     1261 tcgtctttat ctcgtttcgc atgggaagag acgacgtcga ttttccgttg cgcgcggcca
     1321 agcgctcgac cgccgagcgc ctcaaggcga gcggtgtccc atggacggtc atcgaggcgt
     1381 cgatcttcat ggaggtatgg cttgggcccc acctcggctt cgacgcggcg aacggccgcg
     1441 tccgcgtcct cggttccggc gaccagaaga tcagctgggt gtcctatcgc gatgtcgccg
     1501 caatcgccat agccgctgcc gagagcccgg atgcggagaa ccgcacgatc gcggtcggcg
     1561 ggccggaagc gctgtccccg aacgaagtcg tccggatctt cgaagaacgg tcgggccgaa
     1621 agttcgaagt ggagcgcgtg cccgaatccg cgatcgctca gatgagagcg aacgcacgaa
     1681 acccggtcga agagaccttc gcgggactca tgctcttctg cgcacgcggc gacgagatcg
     1741 acatgcgaca gacgctctcg cgctatccaa tacgcatgac gacagtgcgc gattacgccg
     1801 atcgggtcct ggcgggataa cggacgcgcg ggctccgtgc cgaccccgtt cggaacggat
     1861 cagctcgaga ccgggccggt cgggcgtttt cggctgctct gctcagcgtc gaaagggtgg
     1921 cggccacgcg tgccggccgc ggttcgtcga gcggaacatc ccggcaccgc gatcgtgtgg
     1981 cagcaggaga tcttcgaggt cgtggcggcc gagccccttg ccgaaggagc aatccgctac
     2041 tcgctcgagc cctggagaaa cgatttcgcg atccgctcgc tcgagcgcta cgacggcgag
     2101 agtgagtcgg cgcgagccac cgagcggcgg tggagggccg aagcgccgag aagacgccgc
     2161 ctggcggtcc tcttctcccc cttgctgggg caccttccgg gcagcgttca ggagaagatg
     2221 gaaaaggact tcggcgctcc ggccaacgcg atgaccgtcg tttccgcctt tccgctcttc
     2281 gtcgtcggag ccttcggtct cttcgcgttc atcgcccgcc tcgcgggagg ctccctcacg
     2341 ccgctcccgg agccttccct cccgctgtcg ctgtaccttg tcggggagtc atccctgcga
     2401 cttgtcatcg tggtcagcca gtcgcggccg gtcggctcga ttccggggat actcctgtac
     2461 ggcatcggaa gcagaatcgt cgcccggctg agaggaacgg ctgttgcatc aggaggtcgc
     2521 ccatgaaatc caccccgact cggtttcttc tcttcatggt tctcgtcggt atcgccccgg
     2581 tcttgctcgc tgcgcccggc gaggcgcgca agatcgaggt cgtggcgcac aagtacgctt
     2641 tcgatccgtc gaaaattgag atcaaggccg gagagaccgt cgagatcact ttccgcagca
     2701 cggacacgaa acacggcttc gtgtgcaagg aactcggcct cgagaaagtc gtcttcacca
     2761 aagagaagcc ggcgacggtc accttcacgc cagacaagcc cggaacctat cccttcaagt
     2821 gcgcgaggtt ctgcggcctc ggccacccga agatgaaggg acagattgtt gtgacgccgt
     2881 aggccgctcg cggaactctc gttcatcgac gacctcgccg gcggtcgtgg cgaagttcta
     2941 gctgggcgag accgcgggcg acgtcgcctt cggctcgacg accggggcga tgtcgtcttc
     3001 gggcagaacg cccatgagct tccggatcgg tttcgcgagc acgagcagga cgagtccggc
     3061 gacgcccgtc accttcgcga ccgacaggaa gagctcctcg acccccattc cctcaaagcc
     3121 tccggctgcg agacccgcga tcaggttccc gagcgccgtc cccatgaacc atgttcccat
     3181 catctggccc acgaggcttc gcggcgaaag cttggtgacg ctcgagagcc cgaccgggga
     3241 aagacagagc tcgccgacgg tgtggagcag gtacgtgagg atcagccaca tcatgctcac
     3301 gcgcgcgccg cccgccgtga aactggcgcc ccacgccagc acgaggaaac cgatggcgag
     3361 gaatgccagc ccgaacccca tcttcgccgg agtggagggt tgcttgcggc cgagacgcac
     3421 ccagagcgct ccgaagacgg gcgcgagaag gatgatgaag atcgaattca ccgcctggag
     3481 gaatgaggcg ggcatctccc agccgagaat tacgcgctgg gtcattcgct cgccgaagag
     3541 gttcatcgag gagcccgcct gttcgaaccc ggcccagaag aggaccgccg ccatgaagaa
     3601 cacgaagatg acggcgatgc gcttcttctc ggcccgtgag agccgcgaaa ggaaaatgat
     3661 cgcgccgaag tagacgagcg cgagcccgag catgaagaag ccgacgcctt tcgcgacgcc
     3721 gtacaggtcg aggtggacga tgcccgcgag ctgaagcagt acggcaagga cgatcgcgcc
     3781 gaggacggcg gccgctgcga gcgccagctt ccggacggcg ctccgatcgc tcgcggacgt
     3841 cacctcggac gagcgacgca gcccggcttc cccgaggtac ttccctccga ggcggtattg
     3901 cacgaggccg aggaccatgc cgaccccggc ggccccgaag ccgaggtgcc agttcacttt
     3961 ctcccccagg aacccgcaca cgagcggccc gagcatcgct ccgacgttga tccccgagta
     4021 gaagatggag aaacccgcgt cccggcggtt tccgccttcc gggtagaggt cggcgaccat
     4081 cgcgctcacg ttcggcttca agagccccgt gccgatcacg atgagacaga gacccagata
     4141 gaaggtcgcg accgacccga tggccatgct gaagtgcccg gctgcgatga agcacccgcc
     4201 gatgaacacg gcgcgccgct ggccgaggat ccggtcggcg acccacccgc ccggcagcgc
     4261 catcagatag acgagtccga cgtacatgcc gtaaatcgcg gcgcccgtgc ggtccgtgag
     4321 tccgaacccg ccgttctgga tcgtcgcgac gaggaagagg aggaggatcc cgcgcatccc
     4381 gtaatagctg aacctctccc acatctcggt gaagaagagc gtggagagac cgcgcggatg
     4441 cccgaaccat ttcttgtccg cgggattggc ctgcgtcatt tcgccccctc ggtccgacag
     4501 gctaccagag ccggtcggct tctcaggttc gaacgttcgc ccgccggcta ccgattcccg
     4561 gtcgccgttt cggtgaggct ctcgccgacc gcgcgggcgg tccggcggag atcggcgtcc
     4621 gagtgcgcgg tcgaaacgaa ccacgcttcg aactgcgccg gaggaaggaa gacgccgcgc
     4681 tcccgcatcg cgtggaagaa ggcgccgtac cgcgcggtgt ccgagcgcct ggccgaggtg
     4741 aaatcggtga ccggacctcc ggtgaagaag agggtcgaga gagagccgac ccgctggtag
     4801 gttacgcagt ccccggctcc ggcttcgcgg atcgccttgc tcacctccga ttcgaggagc
     4861 gcgcccttgc gctcgagctc cgcgtaggga cttcgacggt cgatctcgcg caacatcgcg
     4921 atcccggccg ccatcgcgag cggattgccg gagagagttc ccgcctggta cacgccgccg
     4981 gaaggcgcga cctgcgccat caggtcgcga cggccgccgt atcctccgac cggcagtccc
     5041 ccacccagga tcttcccgag gatcgtcagg tccgcgctca cgccgtagag ctcctgtgcg
     5101 ccgccgcgcg ccacgcggaa tcccgtgatg acctcgtcga agacgaggag agcgccgtgt
     5161 ttaaaggtga gctcgcgcgc tctctcgaga taccccggcc gcggcggcac gacacccatg
     5221 ttcgcggcga ccggctcgag aacgaccgcg gcgatctcgc cacggtgccg gcggaaggcc
     5281 tcttcgagtg cgtcgccgtc gttgtaaggc acgaccgtgg tcag
//
LOCUS       DASWAV010000695         5100 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U2665
            k141_399207, whole genome shotgun sequence.
ACCESSION   DASWAV010000695 DASWAV010000000
VERSION     DASWAV010000695.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960316
            Sequence Read Archive: SRR10260007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 5100)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5100)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:47:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,929
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5100
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_399207"
                     /isolate="SMAG_U2665"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.36444 N 132.31889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..404
                     /locus_tag="VF958_14695"
     CDS             <1..404
                     /locus_tag="VF958_14695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="nucleotide-binding protein"
                     /protein_id="HEX9150402.1"
                     /translation="DIFLVKYSSNGMHQWSRSYGTTAAVGDIGNAVAVDGANNVLLTG
                     SIVGAVDFGGGALAGNGSYDVFIAKFRSDASHLWSKRAGALYDDHGWGIAADSTGNVF
                     STGDFYQSINFGGGTLVNEAGTDSYVVKLSP"
     gene            complement(416..1855)
                     /locus_tag="VF958_14700"
     CDS             complement(416..1855)
                     /locus_tag="VF958_14700"
                     /inference="COORDINATES: protein motif:HMM:NF017044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HEX9150403.1"
                     /translation="MALLAIAVTAGAAKQTPAPPGPDRSKPPTPGPVRLLKLPPVQQL
                     SLSNGLPVLLLEMHEVPVIQVNVVVRAGAGTDPLDRPGLASLTADMLDEGAGKASALQ
                     ISDELDYLGADLQARAGWDETTVALHVPLKRFGAALPIFADVVLRPTFPEADLERVRK
                     DRLTELLQLRDEPRAIASVAFANALYGRGHRYGTPTIGTEASVRRFSRADLAAFHSRA
                     FQPGNAAIVVVGDVTAASLLPQIEQAFGGWRPATGGPPATVPAASQVRTRGVWIVDRP
                     GSAQSEIRIGRIGPPRATPDYFALTVMNTILGGSFTSRLMQNLREQHGYAYGARSSFD
                     YRLSTGPFVAAAAVQTDKTAPALAEFFKELDAIRKTVTEAEVAKARNYVALSFPSEVE
                     TTGDIASKLEDQFVYGLPAGWLDSYVSNIGVVTLSDVKRVAEQYVDPAKVAVVVVGDR
                     RKIESEVRALNLGPIKFQTLAEVFGAGPR"
     gene            complement(1876..3258)
                     /locus_tag="VF958_14705"
     CDS             complement(1876..3258)
                     /locus_tag="VF958_14705"
                     /inference="COORDINATES: protein motif:HMM:NF017044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HEX9150404.1"
                     /translation="MTRRLALIVFCAVVLVVVPATARGQGTPRLAIPYTQFTLPNGLD
                     VILHEDHSVPLVAVDAWYHVGSAREKPGRTGFAHLFEHIMFEGSGHVPEGDFDNWLEA
                     AGGDNNGSTDNDRTNYWETVPSNALELALFLESDRMGYLLDAMGPEKVNGQRDVVKNE
                     RRQSYENRPYGVAFLKLPEEMYPPTHPYHWPVIGSMEDLTAASYEDVVQFFKSYYGPN
                     NTTLVVAGDIDPVETRRLVEKWFSDVPRGAPVAPLSAPPAVLAGEKRITLEDKVQLPQ
                     LFIAWHTPPYFHPGDEAMDVLARVLASGKNSRLYKRLVYDLQIAQDVSASQNSGALGS
                     MFLVTATAREGHGLSEIEKVIGEELAKLESEPPAIRELERAVNQIESAFYGALERVGG
                     FGGKADRLNLYEFYTGRPDYFTEDLARYTSLDPRDLQSAARTYLRDDARVVLSVVPTG
                     KKSLAAEKQP"
     gene            complement(3302..4351)
                     /locus_tag="VF958_14710"
     CDS             complement(3302..4351)
                     /locus_tag="VF958_14710"
                     /inference="COORDINATES: protein motif:HMM:NF013696.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HEX9150405.1"
                     /translation="MIAGRMPDAGERAVELLTELVAIPSVTGSEKALLEFLERRYAAS
                     GWTVEPMDVAPDRRNLFVHRGHPSVVLTTHADTVPPFFPPRREGDVLVGRGACDAKAS
                     LAAQAVALEELAREGSGEVGLLVLVGEERGSDGALAANRRPHLARYLVGGEPTGSRFV
                     AGSKGCLRIEVEARGVSGHSSEPSTGRSAVDPLLDFLNDLRALKFPEHRVFGATTMNI
                     GVLAAGTAPNVIAESARAEIIFRTGEPIETLLAHIRPMAEGRVELRVGYRSDPVSFRC
                     PKANPGEIVSFACDLPLLPAWGEPILFGPGSIRDAHGAEEKVSLPEVAAAVGIYVGLV
                     RGLLTQGETCLAPRK"
     gene            4505..>5100
                     /locus_tag="VF958_14715"
     CDS             4505..>5100
                     /locus_tag="VF958_14715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacylase"
                     /protein_id="HEX9150406.1"
                     /translation="MRALVVAVAVSLPLLAATPPKTADLDDLVFEGGHVVDGTGAPWF
                     VADVSVRDGKISGVGRLDKWPARRRIDARGLVVAPGFIDLLGQSEYNVLVDPRAASKI
                     TQGITTEVTGEGVSIAPVNQKMIDDSRDVYAKYGVWPDWRTLDEYLRTLERRGTAINL
                     GTFIGSGGLRSYVVGRENRPATSTEIASMKALLEQGMR"
BASE COUNT          829 a         1734 c         1729 g          808 t
ORIGIN      
        1 ccgacatctt cctcgtcaag tactcctcga acggaatgca ccagtggtcg cggagctacg
       61 gcacgacggc ggcagtcggc gacatcggga acgcagtggc tgtcgacgga gcaaacaacg
      121 tccttctcac cggttcgatc gtgggcgccg tcgacttcgg cgggggtgcg ctcgccggca
      181 acgggagcta cgacgtcttc atcgcgaagt tccgttccga cgccagccac ctctggtcga
      241 agcgagccgg cgcgctctac gacgaccacg gctggggcat cgccgcggac tcgaccggga
      301 acgtcttctc caccggtgac ttctaccagt cgatcaactt cggcggaggc accctcgtga
      361 acgaggcggg caccgactct tacgtcgtca agctctcccc gtagctcgag ctcccctacc
      421 gcggtccggc gccgaaaacc tcggcgagcg tctgaaactt gatgggcccc agattcaagg
      481 cccgcacttc cgattcgatc ttccgccgat cgcccacgac cacgaccgcg accttcgcgg
      541 ggtcgacgta ctgttctgcg acgcgcttca cgtcggagag cgtgaccacg ccgatgttcg
      601 agacatacga gtcgagccag ccggccggca gcccgtagac gaactggtct tcgagcttcg
      661 atgcgatgtc gccggtcgtc tcgacctccg acgggaaaga gagcgcgacg tagttccttg
      721 ccttcgcgac ctctgcttcg gtgaccgtct tgcggatcgc gtcgagctcc ttgaagaact
      781 cggcgagcgc cggcgccgtc ttgtcggtct gaaccgccgc cgcggctacg aacggtccag
      841 tcgaaagccg gtagtcgaag gacgatcgtg cgccgtacgc atagccgtgc tgttcccgca
      901 ggttctgcat gaggcgtgaa gtgaacgaac cccccaggat cgtgttcatg acggtcagcg
      961 cgaagtagtc gggagttgcg cgcggcggtc cgatccgccc gatgcggatc tccgactggg
     1021 cggagcccgg ccggtccacg atccagactc ctcgcgtccg aacttgcgag gccgcgggca
     1081 ccgttgccgg aggcccaccc gtcgcgggtc tccacccgcc gaaggcctgc tcgatctgcg
     1141 gcaggagcga ggcggccgtc acgtctccca cgacgacgat cgccgcgttg cccggctgga
     1201 atgcacggga gtgaaaagcc gccaggtcgg cgcgcgaaaa acggcgcacc gaggcctccg
     1261 ttccgatggt tggagtgccg taccggtgac cgcgcccgta cagcgcgttc gcaaacgcga
     1321 ccgaggcgat cgctcgcggt tcatcgcgca actgcagaag ctcggtcagg cggtccttcc
     1381 ggacccgctc gagatcggcc tccgggaacg tgggccggag cacgacgtcg gcgaatatcg
     1441 gcaacgccgc accgaacctc ttcaagggaa cgtggagggc gacggtcgtc tcgtcccagc
     1501 ccgcccgggc ctggaggtcg gcgcccaggt agtcgagctc gtcggagatc tgcagggcgc
     1561 tcgcctttcc ggcaccttcg tcgagcatgt ccgcggtcag gctcgcgagt cccggccggt
     1621 cgagagggtc cgtgccggcg ccggcgcgca cgaccacgtt cacctggatg accggcacct
     1681 cgtgcatctc gaggagcagg accgggagcc cgttgctcag gctcagctgc tgcacggggg
     1741 gcagcttcag caggcggacg ggtccggggg tcggaggctt cgagcggtcc ggtccgggcg
     1801 gcgccggcgt ctgcttcgcc gccccggcgg tcaccgcaat cgccaagagt gccaagaccc
     1861 tcgcggcccc ccgcctcatg gctgcttctc cgccgcgagc gacttcttcc cggtcgggac
     1921 gactgagagc acgacgcgcg cgtcgtcgcg cagataggtg cgagccgcag actggaggtc
     1981 ccgcggatcg agtgaggtgt accgcgccag atcttcggtg aaatagtcgg gccggcccgt
     2041 gtagaactcg tagaggttca gacggtcggc cttgccgccg aacccgccga cgcgctcgag
     2101 cgcgccgtag aaggccgact cgatctggtt gacggcgcgt tcgagctcgc ggatcgcggg
     2161 aggttcgctc tcgagcttcg cgagctcttc accgatcacc ttctcgatct cggagagtcc
     2221 gtgtccttcg cgggccgttg ccgtgacgag gaacatcgac ccgagcgcgc ctgagttctg
     2281 gctcgcggag acgtcctgcg cgatctggag gtcatagacg agccgcttgt agagccggga
     2341 gttcttgccg gacgcgagca ctcgagcgag cacgtccatc gcctcgtcgc cggggtggaa
     2401 gtagggcggc gtgtgccagg caatgaagag ctggggcagc tgcaccttgt cttcgagcgt
     2461 gatccgcttc tcgccggcga gcactgccgg cggtgcggag agcggcgcaa ccggcgcgcc
     2521 tcggggcacg tcggaaaacc acttctcgac gaggcgccgg gtttcaaccg gatcgatgtc
     2581 cccggccacg acgagcgtcg tgttgttcgg cccgtagtag ctcttgaaga actgcacgac
     2641 gtcctcgtag ctcgccgcgg tcaggtcctc catcgacccg atcacgggcc agtggtacgg
     2701 atgcgtcggc gggtacatct cctcgggtaa tttcaggaac gcgacgccgt acgggcggtt
     2761 ctcataggac tgccggcgct cgttcttgac gacgtcccgc tggccgttga ccttctcggg
     2821 gcccatcgcg tcgagcagat atcccatgcg atcggactcg aggaacagcg cgagctcgag
     2881 cgcgttcgaa gggacggtct cccagtagtt cgtccggtcg ttgtcggtcg atccgttgtt
     2941 gtcgcctcca gccgcctcga gccagttgtc gaagtcgccc tcgggcacgt ggccggagcc
     3001 ttcgaacatg atgtgctcga acaggtgggc gaaaccggtg cggcccggct tctcgcgcgc
     3061 ggaaccgacg tgataccacg cgtccacggc gacgagaggc acgctgtggt cctcgtggag
     3121 gatgacgtcg agaccgttgg gaagcgtgaa ctgcgtatac ggaatggcga gccgcggtgt
     3181 cccctggccc cgcgcggtcg cggggacgac gacgagaaca actgcgcaga agacgatcag
     3241 agcaaggcga cgcgtcatgt ctcccccaat cccgagaagt tacggtccca cacggcgaaa
     3301 gttactttct cggcgccagg caggtctccc cctgcgtgag cagtccgcgg acgaggccga
     3361 cgtagatccc gacggcggcc gcgacctccg ggagcgaaac cttctcttcc gccccgtgcg
     3421 cgtcgcggat cgagccgggc ccgaagagga tcggctcgcc ccacgcggga aggagcggga
     3481 ggtcgcaggc gaacgagacg atctcgccgg ggttcgcctt cgggcagcgg aacgagaccg
     3541 gatcggatcg gtagccgacc cggagttcca cacggccctc cgccatcggc cggatatggg
     3601 cgaggagggt ctcgatcggc tcccccgtcc gaaagatgat ctcggcgcgc gcgctctccg
     3661 cgatgacgtt cggggccgtg ccggccgcga gaaccccgat gttcatggtc gtggcgccga
     3721 agacccgatg ctccgggaac ttcagcgccc ggaggtcgtt caagaaatcg aggagcggat
     3781 cgaccgccga ccggccggtc gagggctcgg aggaatgccc cgacacgccg cgcgcctcga
     3841 cctcgatccg caggcacccc ttcgatcccg cgacgaaccg gctgccggtc ggctctcccc
     3901 ccacgaggta gcgggcgagg tgcggccggc ggttcgcggc gagcgcgccg tccgaacccc
     3961 gttcctctcc gacgagcacg agcagcccga cttcgccgct tccttcgcga gcgagctcct
     4021 cgagcgcgac cgcctgcgcg gcgaggctcg ccttcgcgtc gcacgccccc cggccgacga
     4081 gcacgtcccc ttcacgacgg ggcgggaaga acggcgggac ggtgtcggcg tgtgtcgtca
     4141 gaacgacgct cggatgcccg cgatggacga agaggttgcg gcggtccggc gcgacatcca
     4201 tcggttccac cgtccagccg gaggcggcat atcgtcgctc gagaaactcg agcagagctt
     4261 tctccgatcc cgtcaccgac gggatcgcga cgagctcggt caggagctcg acggcgcgct
     4321 cgcccgcatc cggcatacgc cccgcgatca tagactgcgc cgtatgaaca gaaatcgacc
     4381 gcgccgccac aggaactccc tctcccccac cgggggtgcg cgaggggtct cccctcgcag
     4441 acaggatgtg ccggccgcga ccgaaggtcg cgtttctcgc gaagcgagcg gccggcgggg
     4501 cggggtgagg gcactggtgg tcgctgttgc agtctctctg ccgctcctcg ccgcgactcc
     4561 tccaaagacc gcagacctcg acgacctcgt gttcgagggc ggccacgtcg tggacggaac
     4621 cggcgcgcca tggttcgtgg cggacgtgtc ggtccgcgac gggaagatct ccggcgtcgg
     4681 ccggctcgac aagtggccgg cgcggcgccg gatcgacgcg cgcggtctgg tcgtcgcgcc
     4741 cggtttcatc gacctgctcg gccagtccga atacaacgtg ctcgtcgacc cgcgcgccgc
     4801 ctcgaagatc acgcagggca tcacgaccga agtcacgggc gagggcgttt cgatcgctcc
     4861 ggtcaaccag aagatgatcg acgactcgcg cgacgtctac gcaaagtacg gtgtctggcc
     4921 ggactggcgc accctcgacg aatacctccg gaccctcgaa cgccgcggaa cggcgatcaa
     4981 tctcggcacg ttcatcggct ccggaggcct gcggagctac gtcgtcggcc gcgagaatcg
     5041 cccggccacc tcgacggaga tcgcttcgat gaaggcgctc ctcgaacagg ggatgcggga
//
LOCUS       DASWAV010000696         4427 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U2665
            k141_399518, whole genome shotgun sequence.
ACCESSION   DASWAV010000696 DASWAV010000000
VERSION     DASWAV010000696.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960316
            Sequence Read Archive: SRR10260007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 4427)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4427)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:47:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,929
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4427
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_399518"
                     /isolate="SMAG_U2665"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.36444 N 132.31889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..230
                     /locus_tag="VF958_14720"
     CDS             <1..230
                     /locus_tag="VF958_14720"
                     /inference="COORDINATES: protein motif:HMM:NF024773.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HEX9150407.1"
                     /translation="DIVDVFRRSDQVGAIADAAIARGVGFFFMQQGVVDPKAARRLEA
                     AGIPVAMDRCILVEHVSRALPPRNFEARRQR"
     gene            348..1871
                     /locus_tag="VF958_14725"
     CDS             348..1871
                     /locus_tag="VF958_14725"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: protein motif:HMM:TIGR02037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Do family serine endopeptidase"
                     /protein_id="HEX9150408.1"
                     /translation="MEKSTKRAISFSVLIAAAVIFGMVVASSVNVTPRSEAQRDAPAR
                     AARTGSMLIPSFADIADETMSSVVSITSTEIVKGSSMRRNPFSGQGDPFEFFFGPQQR
                     RRDQSDDEEHKEQQGGTGFIISDDGYVVTNYHVVENADKVEVRLSNRDRLEARIVGRD
                     PATDLALLKIDVKQHLNPLPLGDSDRLRVGEWVMAIGDPLNFDKTVTVGVVSAKGRFG
                     LTADQATRSFENFIQTDAAINFGNSGGPLINVNGEVIGINTAMFRPAQNIGFAVPVNT
                     LKSVLPQLREKGKVIRGFLGINIENIDSDKASAFKLKSQDGAFVQSVEDGKPAERAGV
                     KPGDAIVRVDDVPVKDTRDLIGYVSGKAPGSKVRLGIVRDGREMNLTATLAERDVASA
                     EGQDNSGKASSEDSRERIGISVTELTPQVRQMQGIPGNTNGLLVVHVKEVSPAADAGI
                     REGDVITEVNGRRATSTDEFGKLVAGSRKGDYLKLYILRPARSGASVSFFALVKIEQ"
     gene            2036..3397
                     /locus_tag="VF958_14730"
     CDS             2036..3397
                     /locus_tag="VF958_14730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006002658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HEX9150409.1"
                     /translation="MSHKILVVDDDAGIRDTLRMILEYEGYEVATSADGKTALADLDA
                     SPADAVLLDIKMPGMDGLEVLDRIVARDGAPPVLMISGHGDIATAVESTRRGAIDFLE
                     KPPQRERILVSLKNALSRNQLRVENQRLRMALEEESVLVGKSAPMLRLRQEIVRAAPT
                     GATVLIMGESGTGKELVAREIHAASRVSGGPFIQVNCAAIPEELIESELFGHEKGSFT
                     GAVRRQTGKFVEADGGTIFLDEVGDMSARTQAKVLRVLEAGEVEPVGAARVRQVNVRV
                     IAATNRDLTEAIRDGRFREDLFFRLNVVPIRTPPLRERTEDIPELVEHFTRRFIERDH
                     RRPRRFAPSALALLKARSWPGNVRELKNLVERVLIMTDADPIEAKDLPPEARVAPSEI
                     SERGLAMTTLSEFKEFAERQFLMAKLRENGWNISRTAEVIQTPRSNLYKKIDHHKISR
                     DQQ"
     gene            3493..4059
                     /gene="pyrE"
                     /locus_tag="VF958_14735"
     CDS             3493..4059
                     /gene="pyrE"
                     /locus_tag="VF958_14735"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013536840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004588 - orotate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HEX9150410.1"
                     /translation="MLTIDDFIEAGALLEGHFRLSSGLHSDRYLQCARLLMWPDRAER
                     AGRELAGRLAEFSPGAIVSPALGGVIIGHEVARTLGVRAIFAERRDGAFALRRGFAIA
                     PGERVAVVEDVFTTGGSTRDVCGAGAAAGGEVVAVGSLVDRGIPAGAFSVPARSVLTL
                     SVPSWRPEDCPLCAEGVPLDTPGSRHAG"
     gene            4069..>4427
                     /locus_tag="VF958_14740"
     CDS             4069..>4427
                     /locus_tag="VF958_14740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007513260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HEX9150411.1"
                     /translation="MELTRICVYCGASAARDPAVETEVRVFGREIARRGIGLVYGGSS
                     LGLMGALADEVLASGGKVIGVIPRALVGREAAHGGLSELRIVETMHQREQVMFDLGDA
                     FVTFPGGFGTIKEFLEMV"
BASE COUNT          755 a         1433 c         1502 g          737 t
ORIGIN      
        1 tcgacatcgt ggacgtcttc cgacggtccg accaggtcgg agccatcgcc gacgcggcga
       61 tcgcacgagg tgtcggcttc tttttcatgc agcagggcgt cgtggacccg aaagccgccc
      121 ggcggctcga ggcagccggg atccccgtcg ccatggaccg ctgcatctta gtcgagcacg
      181 tgagccgggc gttgcctccg aggaatttcg aagctcgcag gcagcgttaa gtcggtgacc
      241 ggcagccacg cggttccagc ggagttgccg ggatcggttt tgaaccttga aacctcgaac
      301 ctgagccttg aacctgtgaa gcgggtataa ggagtagaag agaaaccatg gagaagtcca
      361 ccaagagagc catttccttt tcggtcctga tcgcggcggc cgtcatcttc ggcatggtgg
      421 tcgcgagctc ggtcaacgtc actccgcgtt ccgaggcgca gcgcgacgcg ccggcccgcg
      481 cggcccgcac cggctcgatg ttgatcccgt ctttcgcgga catcgccgac gaaacgatgt
      541 cttcggtcgt ttcgatcacc tcgacggaga tcgtcaaggg gtcgtcgatg cgtcgcaacc
      601 ccttcagtgg ccagggcgac ccgttcgagt tcttcttcgg accccagcag cgccgccgcg
      661 accagagcga cgacgaggag cacaaggagc aacagggcgg tacgggattc atcatctcgg
      721 acgacggtta cgtcgtcacg aactatcacg tggtcgagaa tgccgacaaa gtcgaggtcc
      781 gtctttcgaa tcgcgaccgc ctggaagcgc ggatcgtcgg gcgtgatccg gcgaccgacc
      841 tcgcgttgct caagatcgac gtcaaacagc atctgaaccc gctgccgctc ggtgattccg
      901 accggttgcg cgtgggcgag tgggtcatgg cgatcggcga cccgctcaac ttcgacaaga
      961 ccgtgacggt cggcgtcgtc tcggccaagg gccgtttcgg gctgaccgcg gaccaggcca
     1021 cccgttcctt cgaaaacttc atccagaccg atgccgcgat caacttcggc aactccggcg
     1081 gcccgctgat caacgtcaac ggcgaggtca tcggcatcaa cacggcaatg ttccgccccg
     1141 cgcagaacat cggcttcgcc gttccggtca acaccttgaa gagcgtgttg ccgcaacttc
     1201 gcgagaaggg gaaagtgatt cgcggattcc tcggcatcaa catcgagaac atcgacagtg
     1261 acaaggcctc ggccttcaag ctcaagtcgc aggacggcgc gttcgtccag tcggttgagg
     1321 acggaaagcc ggcggagcgt gccggggtga agccgggcga cgcgatcgtg cgcgtcgacg
     1381 acgtcccggt caaggacacc cgcgacttga tcggctacgt ctcgggcaag gcgcccggaa
     1441 gcaaggtccg tctcggtatc gttcgggacg ggcgcgagat gaacctgacc gcgactctcg
     1501 ccgagcgcga cgtcgcgagc gccgagggac aggacaacag cggcaaggcg tcttcggagg
     1561 attcgcgcga gcgcatcggg atctccgtga ccgagctgac cccgcaggtc cgccagatgc
     1621 aggggatccc ggggaacacg aacgggctgc tcgtcgtgca cgtcaaggag gtctcgcccg
     1681 cagccgacgc ggggatccgt gaaggagacg tcatcaccga ggtcaacgga cgtcgcgcca
     1741 cttccaccga cgagttcggc aagctggtgg cgggctcccg caagggcgac tacctgaagc
     1801 tgtacatcct ccggcccgct cgttccggcg cctcggtctc gttcttcgcc ctcgtgaaga
     1861 tcgagcagta agcgccaacc ccgcgagttt cgacagcgcc cgcccctcga ggcgggcgct
     1921 tccatttgac gtcgcgggga caggccctgc gggccagcac ccgcaacctt cttcgggctc
     1981 tgtactcggg aagagacgga gtcccgttgg catttacaca tgcggagatg ggggaatgag
     2041 ccacaagatc cttgtcgtcg acgacgacgc cggtatccgc gacactctga ggatgattct
     2101 cgagtacgag gggtacgagg ttgcgacctc agccgacggg aagaccgcgc tcgccgatct
     2161 cgacgcgagc ccagcagatg cggtcctcct cgacatcaag atgcccggca tggacgggct
     2221 cgaggtcctc gacaggatcg tcgcgcggga cggcgcgccg cctgtattga tgatctcggg
     2281 acacggggac atcgcaacgg ccgtcgagtc gacccgccgc ggtgcgatcg acttcctcga
     2341 gaagccgccg cagcgggagc ggatcctggt ctcgctcaag aatgcgctct cgcgcaacca
     2401 gctccgtgtg gagaaccagc ggctgcggat ggcgctggag gaagagtcgg tgctcgtcgg
     2461 caagagcgcg ccgatgctgc ggctccgcca ggagatcgtg cgcgccgcgc cgacgggtgc
     2521 gacggtcctc atcatggggg agagcggcac gggcaaggag ctcgtcgcgc gcgagattca
     2581 cgcggcttcg cgcgtctccg gcggaccgtt cattcaggtc aactgcgcgg cgattcccga
     2641 ggagttgatc gagtcggagc tcttcggcca cgagaagggc tcctttaccg gggccgtgcg
     2701 tcggcagacc gggaagttcg tggaggcgga cgggggcacg atctttctgg acgaagtcgg
     2761 cgacatgtcg gcgaggacgc aggcgaaggt gctccgagtg ctcgaggcgg gcgaggtcga
     2821 accggtcggc gcggcccggg tccggcaggt gaacgtgcgc gtcatcgctg cgacgaaccg
     2881 cgacctgacg gaagcgatcc gcgatggccg gttccgcgaa gacctctttt tccgcctgaa
     2941 cgtcgtcccg atccggaccc ctccgctgcg ggagcgcacc gaggacatcc cggaactcgt
     3001 cgagcacttc acgcgccgct tcatcgagcg cgaccatcgc cgcccgcgcc ggttcgcccc
     3061 ttcggctctc gcgttgctca aggcgcgctc ctggccggga aacgtgaggg agctgaagaa
     3121 cctggtcgag cgggttctca tcatgaccga cgccgacccc attgaggcga aagacctgcc
     3181 gccggaggcg cgggtcgcgc cttcggagat ctccgagcgc ggcctcgcga tgacgacgct
     3241 ctccgagttc aaggagttcg ccgagcgtca attcctgatg gcgaagctcc gggaaaacgg
     3301 ctggaacatc agccgaacgg cggaggtgat ccagacgccg cgctcgaacc tgtacaagaa
     3361 gatcgaccac cacaagatca gccgggatca gcagtagttt ttccccggtc atccggagcg
     3421 aagcgaggga tctctctctc gaaataggga gagattcttc ggcccctggg gcctcagaat
     3481 gacgggctgg agatgttgac catcgacgac ttcatcgaag ccggtgccct cctcgagggc
     3541 cacttccgcc tctcctccgg cctccacagc gatcgctatc tccagtgcgc gcggctcctc
     3601 atgtggcccg atcgggccga gagagcgggg cgggagctgg cgggcaggct cgccgagttc
     3661 tccccgggcg cgatcgtctc cccggcgctc gggggcgtca tcatcggaca cgaggtcgcg
     3721 cggactctcg gcgtccgggc gatcttcgcc gagcgcaggg acggcgcctt cgccctccgc
     3781 cgcgggttcg cgatcgcgcc gggcgaacgc gtcgccgtgg tcgaagacgt cttcacgacc
     3841 ggcggttcga cccgcgacgt ctgcggggcc ggcgcggccg ccggcggtga ggtcgtcgcc
     3901 gtaggatcgc tcgtcgaccg cggcattccc gcgggcgctt tctccgtccc cgcgcgctcc
     3961 gtcctcaccc tgtccgtccc gtcgtggcgc cccgaggact gtccgctgtg cgccgagggc
     4021 gtcccgctcg acacgccggg tagccgccat gccggttaga ctttccgcat ggagctgacg
     4081 cggatctgcg tctactgcgg tgcgagcgcg gctcgggatc ccgccgtgga aacggaggtc
     4141 cgcgtgtttg gccgcgagat cgcgaggcgc gggatcggcc tcgtctacgg cggttcgtcg
     4201 ctcggtctga tgggcgctct ggcggacgag gtgctcgcct cgggcgggaa ggtgatcgga
     4261 gtgattccac gggctctcgt cgggcgcgaa gccgcgcacg gcgggctctc ggaactccgt
     4321 atcgtcgaga cgatgcacca gcgcgaacag gtcatgttcg atctcggcga cgcgttcgtc
     4381 acgttcccgg gaggcttcgg gacaatcaag gagtttctcg agatggt
//
LOCUS       DASWAV010000697         5123 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U2665
            k141_599211, whole genome shotgun sequence.
ACCESSION   DASWAV010000697 DASWAV010000000
VERSION     DASWAV010000697.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960316
            Sequence Read Archive: SRR10260007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 5123)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5123)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:47:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,929
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5123
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_599211"
                     /isolate="SMAG_U2665"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.36444 N 132.31889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..924)
                     /locus_tag="VF958_14745"
     CDS             complement(<1..924)
                     /locus_tag="VF958_14745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9150412.1"
                     /translation="MASIVLWIACASSGGGPGAPAARRHDEFPLDPRDDLTGPFPEGV
                     AAGWSALAEGRNADAEREFLAARGGRPQLAAEIGLIEARVLLGRTGEALSACREALVG
                     GTQTVPLLVACGEARARAGQVFEAHELYARAAARGGSRPAIRKRADDLKAQAIETLAR
                     TASAGARDRNYSDARKRAERAIELAPGDSTLHALAGEVELEDGQPDRAFERYREASRL
                     DPPNVAVAAKLAALARDRDPSLAVSILDALARRDARYADRATDARLAFRISNWPAPER
                     EAARSERLTRAAAASLVWWMFPEIREARVKSS"
     gene            complement(1005..1226)
                     /gene="hfq"
                     /locus_tag="VF958_14750"
     CDS             complement(1005..1226)
                     /gene="hfq"
                     /locus_tag="VF958_14750"
                     /inference="COORDINATES: protein motif:HMM:NF028518.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006378 - mRNA polyadenylation [Evidence
                     IEA];
                     GO_process: GO:0016070 - RNA metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone Hfq"
                     /protein_id="HEX9150413.1"
                     /translation="MNKTPINVQDSFFYNLRKDNTEITVRLTSGEERMGRLRRFDKFA
                     LVLEVEGHEEMIYKHAIASIRAESLPTTS"
     gene            complement(1223..2173)
                     /gene="miaA"
                     /locus_tag="VF958_14755"
     CDS             complement(1223..2173)
                     /gene="miaA"
                     /locus_tag="VF958_14755"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: protein motif:HMM:TIGR00174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HEX9150414.1"
                     /translation="MTDDATRRPPLLLIFGPTAAGKTAIAEEVARLRSGEIVSADAFA
                     VYRGFDVGTAKPSAERRAEIPYHLIDVADPSETFSAGRWAGQARRAVEDISARGRLPI
                     VCGGSGFYISALIEGLPPGEDRDVDLRRRLARWGRARGPHAAHRFLAQNDPASASRIP
                     IGNLRYTLRALEILLVTGRRASDRAIGSDPWSAKWRVLNVSIEPSREDLYAKIEGRVR
                     QMLDAGWDREVRQLIQDGLSLESNAFGGIGYREVAGWISGRAEREETLREIVAATRRL
                     AKRQRTWFARQRNVLRTTPDAALPAILQLLDSQVETERGG"
     gene            complement(2170..4380)
                     /locus_tag="VF958_14760"
     CDS             complement(2170..4380)
                     /locus_tag="VF958_14760"
                     /inference="COORDINATES: protein motif:HMM:TIGR00361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0009290 - DNA import into cell involved in
                     transformation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA internalization-related competence protein
                     ComEC/Rec2"
                     /protein_id="HEX9150415.1"
                     /translation="MSKPKGRASAIAGLLSCGSLGFFEGRVAVARHEEVGRATFMALP
                     QGAERADRIEGVLVDFWSDAPPRARGRLRAERLRVAGEWRPFPAEVFLFVSGAIPPEG
                     AAVRGDRVSIGGHLRREEIPASERDIALPWTRYRMSVKSSLQIRRESATVLSLLSLPN
                     RTLSARLPPPGSRGAWFERNVRGPLAALLLGRTSQLDRGMVARYRRGGLYHLLVVSGL
                     HVGLAAGLVLAALRLARVGGKPRDAALFVAVFLFVLVGGANAPAVRAGLVVAVFLTAR
                     LLERPIGAGQAAGLSAIVLFAADPAQIYSLGTVLTFAAVAGIGLGTAPIRRCLPARPD
                     WVFSGLATTLAAQCATAPVLFWRFNLVSAGAWLTSPLAIPLSALLIALGAVILLLFAV
                     GLPATLPAIVFGLGSRALETLADRASGLAVPRPTPPLAAVVAIGGATLLAASGPRRLR
                     AAAGALAAAAFLFLAIRRGPAGPSRGFSVEALDVGQGDAILLRWQRRALLVDGGGPFD
                     VEAADFGRTQLLPKLLDRGVTRLDGVLATHPHPDHVLGLLAIVEELPVGSLWVSAGED
                     EGGLFGQLRRVAAKAGVRVSVFSAGSVVRWGDASVSVLHSGGPRRKADAVNNQSVVAV
                     FERGGRRALLTGDAGAPTERELADLGLLRKSDLLKVGHHGSRTSTTPLLLAAVRPRVA
                     LISCGRENRFGHPAPSTLHALREFGVPTLRTDRLSDVRVELAPEATRLEWRGIE"
     gene            complement(4533..>5123)
                     /locus_tag="VF958_14765"
     CDS             complement(4533..>5123)
                     /locus_tag="VF958_14765"
                     /inference="COORDINATES: protein motif:HMM:NF017743.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent RNA methyltransferase"
                     /protein_id="HEX9150416.1"
                     /translation="LREAGEPRLPIVTGGRVFQVSVDSFFQGNRHLSGRLLEDVEEAA
                     TGPPGEALDAFGGVGFFAASLLTAGHVVTSVEGSAPAARDAAKTRLGWPDADRWKIHP
                     SSVAGFLSSSSRRFDRVVADPPRAGLARLARPLAGRARRQLVYVSCEPATLARDLSEL
                     LEEGFEIADARLYDLFPLTHRVEAVVTLLRGARKPA"
BASE COUNT          869 a         1818 c         1798 g          638 t
ORIGIN      
        1 gctggatttg accctcgcct ctcggatttc cgggaacatc caccagacga gagacgcggc
       61 cgccgcgcgg gtcaaccgct ccgacctcgc tgcctcccgc tccggcgccg gccagttcga
      121 gatccggaac gccaggcgag catccgtcgc gcggtcggcg tagcgagcgt cccggcgggc
      181 cagagcgtcc aggatggaca ccgcgaggga aggatcccgg tcgcgcgcga gggccgcgag
      241 cttcgcggca accgccacat tcggcggatc gaggcgagac gcctcccggt agcgctcgaa
      301 cgcccggtcg ggctgaccgt cttcgagctc gacctcgcca gcaagggcat gcagtgtgga
      361 atcccccggg gcgagctcga tcgcgcgctc ggcgcgcttc cgcgcgtcgg agtagttacg
      421 gtcccgcgcc ccggcggaag ccgtgcgagc gagcgtctcg atcgcctggg ccttgaggtc
      481 gtccgcacgc ttgcggatcg cgggacggga tcctccgcgg gcggcggccc tcgcgtagag
      541 ctcgtgcgct tcgaagacct gaccggcgcg cgctcgcgcc tccccgcacg cgacgagaag
      601 cgggaccgtc tgcgtccccc cgacgagcgc ctcccggcat gcggagagcg cctcccccgt
      661 ccgtcccagc aggacgcgcg cctcgatcag accgatctcc gccgccagct ggggacgccc
      721 accgcgcgcc gcaaggaact ccctctccgc gtcggcgttc cggccttcgg cgagcgccga
      781 ccacccggcc gccacgcctt cgggaaacgg gccggtgaga tcgtcgcgcg gatcgagggg
      841 gaactcatcg tgacggcgcg ccgcgggtgc cccgggacct ccccccgagc tcgcgcaggc
      901 gatccacagg acgatcgagg ccatggaaat gagagacagt gcgcgtccga tgcgcggccc
      961 gtccggccat cgtcgcgtct ccgcggcagc cggagacctc ctcttcacga ggtcgtcggc
     1021 aagctctccg ctcgaatgga agcaatcgcg tgcttgtaga tcatctcttc gtggccttcc
     1081 acctcgagaa cgagggcgaa cttgtcgaac ctccgaagtc gtcccatccg ctcctctccc
     1141 gaggtgagcc ggaccgtaat ttccgtgttg tctttccgca ggttgtagaa gaacgaatcc
     1201 tggacgttga tcggggtctt gttcatccgc ctctctccgt ctcgacttga gagtcgagca
     1261 actggaggat tgccggaagg gctgcgtcgg gcgtcgtccg caggacgttc cgctgccggg
     1321 cgaaccaggt cctctggcgc ttggcgaggc gccgagtggc cgccacgatc tccctcaggg
     1381 tctcctccct ttccgcgcga cctgaaatcc agccggccac ctcgcgataa ccgatgccgc
     1441 caaacgcgtt cgattcgagg gaaagcccgt cctggatcag ttgccgcact tcccggtccc
     1501 accctgcatc cagcatctgg cggactcgac cttcgatctt agcatagagg tcctcgcgcg
     1561 acggctcgat ggagacgttg agcacgcgcc acttcgcgct ccaggggtcc gacccgatcg
     1621 cgcgatcgga cgcacgccgg ccggtcacga gaaggatctc gagggcacgg agggtgtagc
     1681 ggagattccc gatcgggatc ctcgacgcgg aggcggggtc gttctgcgcg aggaaccggt
     1741 gcgcggcatg cggtcctcgc gctcttcccc accgtgccag ccggcgtctc agatccacgt
     1801 cgcggtcctc tcccggcggc agcccttcga tgagagcgga gatgtagaaa ccgctcccgc
     1861 cgcacacgat cgggagccgc ccgcgcgcgg agatgtcctc gaccgctcgc cgcgcctgtc
     1921 ccgcccagcg cccggcggag aacgtttccg aaggatcagc gacgtcgatc aggtgatacg
     1981 ggatctcggc gcggcgctcc gcggagggct tcgccgtccc gacgtcgaaa cctcgataaa
     2041 cggcgaacgc atccgccgac acgatctcgc ccgatcgaag acgcgcgacc tcttcggcaa
     2101 tcgcggtctt tcccgcggcc gtggggccga aaatgaggag aagcggcggc cggcgggtgg
     2161 cgtcgtcggt cattcgatcc cccgccactc gaggcgcgtc gcttccggag cgagctccac
     2221 gcgcacgtcg gagaggcggt cggttcgaag cgtgggcact ccgaactcgc ggagcgcgtg
     2281 gagggtggac ggcgcgggat gtccgaaccg attctcgcgc ccgcaggaga tgagggcgac
     2341 gcgcggccgc acggcggcaa gcagcagcgg cgtcgttgag gtgcggctgc cgtgatgtcc
     2401 caccttgaga agatcggact tcctcaggag acccaggtcg gcgagctcgc gctcggtggg
     2461 cgcgcccgcg tcgcccgtca ggagcgcgcg gcgcccgccc cgctcgaaga cggcgacgac
     2521 ggactggttg ttgacggcgt ccgccttgcg ccgcggaccg ccggaatgaa ggacgctcac
     2581 cgacgcgtcc ccccaccgga ccacgcttcc tgccgaaaaa accgacaccc gcactcccgc
     2641 cttcgcggcc actctcctca gctggccgaa gagacccccc tcgtcctccc ccgccgaaac
     2701 ccacagcgac cccacaggaa gctcctccac gatcgcgagg aggccgagaa cgtggtccgg
     2761 atgcggatga gtggcgagga cgccgtcgag ccgcgtcaca ccccggtcga gaagcttcgg
     2821 gagaagttga gtccgaccga agtccgcggc ctcgacgtcg aacggaccgc cgccatcgac
     2881 gagaagggcg cgccgctgcc agcgcagcag gatcgcgtcg ccctggccga cgtcgagcgc
     2941 ttcgaccgag aagcctcggg aaggccctgc cgggccgcga cggatcgcga ggaaaaggaa
     3001 tgcggcagcg gccagcgctc ccgcggcggc gcgcagccgg cgaggtccgc tcgcggcaag
     3061 aagcgttgcc ccgccgatgg cgacgacggc ggcgagcggc ggcgtcgggc gcgggaccgc
     3121 aagaccggat gcccggtcgg cgagggtctc gagcgcgcgc gagccgaggc cgaagacgat
     3181 cgcgggaagc gtggcgggaa gcccgaccgc gaagaggaga aggatcacgg cgccgagcgc
     3241 gatcagcagg gcggagagcg ggatcgcgag cggcgaagtc agccaggcgc cggccgacac
     3301 gaggttgaag cgccagaaca gaacgggcgc cgtcgcacat tgcgcggcga gcgtagtcgc
     3361 gagccccgag aagacccagt ccggccgagc ggggaggcat cgccgtatgg gcgcggtccc
     3421 caggccgatg cccgcaaccg cggcgaaggt gagcaccgtg cccagcgaat agatctgcgc
     3481 cggatcggcg gcgaagagaa cgatcgccga gagacccgcc gcctggccgg cgccgatcgg
     3541 ccgctcgagg agccgcgcgg tgagaaagac ggccacgacc agtccggcgc gcacggcggg
     3601 cgcattagcg ccgcccacga ggacgaacag aaaaacggcg acgaagagcg cggcgtcgcg
     3661 cggtttgccg ccgacgcgcg cgaggcggag agccgcgagc acgagaccgg ccgccagccc
     3721 gacgtgcaga cccgacacca cgagcagatg gtaaagcccg ccccggcgat atcgggcgac
     3781 catgccacgg tcgagctgcg atgttcttcc gaggagaaga gctgccagcg gcccgcggac
     3841 gttccgctcg aaccaggctc cacgagatcc cgggggcgga aggcgcgcgg aaagggtccg
     3901 attcggcagc gagagaagcg agaggaccgt tgcgctctcg cggcggatct gaagcgagct
     3961 cttcaccgac atccggtagc gtgtccacgg aagcgcgatg tccctctcgg acgccgggat
     4021 ctcctcgcgg cgcaggtgcc cgccgatcga gacccggtcg ccgcggacgg cggcgccctc
     4081 cgggggaatc gctccggaga cgaaaaggaa gacctcggcg ggaaagggac gccactcgcc
     4141 cgcgacgcgg agacgctcgg cccggagacg tccccgcgcc cgcggcggcg catcgctcca
     4201 gaaatccacg aggactccct cgattctgtc ggcgcgttcg gccccctgcg gcagcgccat
     4261 gaatgtcgca cgtccaacct cttcgtgccg ggccacagcc acgcgaccct cgaagaaccc
     4321 gagagagccg caggagagga gacccgcgat cgcgctcgcc cggcccttcg gcttgctcac
     4381 gaggcgcagc aacgcgagaa gggccgagat cccgcagaga aggagcgccg cgccttcacc
     4441 gctgatggcc cgcgctccga gggcaatccc gacgacgagc gcagccgccg cgggaagcgc
     4501 cggcgcgggg ccggcccgaa ggaccgggcc gatcaggcgg gcttgcgggc gccgcggagg
     4561 agcgtgacga ccgcctccac ccggtgtgtc agcggaaaga ggtcatacag gcgggcatcc
     4621 gcgatctcga agccttcctc caggagctcc gacaggtctc gggcgagcgt cgccggctca
     4681 caggagacat aaacgagctg gcgacgggcg cgcccggcga gcggacgcgc cagtcgcgcc
     4741 agaccggcgc ggggaggatc ggcgaccacc cgatcgaagc ggcggctcga ggaggagaga
     4801 aaccccgcga cggatgaggg atggatcttc catcggtcgg cgtccggcca gccgagccgg
     4861 gtcttcgcgg cgtcccgcgc ggccggagcg cttccttcga cggacgtgac tacgtgtccc
     4921 gcggtcaaga gcgaagcagc gaagaagcca acccctccga aggcgtcgag cgcctccccc
     4981 ggcggacccg tggccgcttc ctcgacgtcc tccagcagcc ggcccgagag atggcggttt
     5041 ccctgaaaga acgagtcgac cgaaacctgg aagacccggc cgcccgtcac gatcggaaga
     5101 cgcggctcgc ccgcctcgcg aag
//
LOCUS       DASWAV010000698         2918 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U2665
            k141_399893, whole genome shotgun sequence.
ACCESSION   DASWAV010000698 DASWAV010000000
VERSION     DASWAV010000698.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960316
            Sequence Read Archive: SRR10260007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2918)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2918)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:47:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,929
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2918
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_399893"
                     /isolate="SMAG_U2665"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.36444 N 132.31889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2084
                     /locus_tag="VF958_14770"
     CDS             <1..2084
                     /locus_tag="VF958_14770"
                     /inference="COORDINATES: protein motif:HMM:NF012485.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="secretin N-terminal domain-containing protein"
                     /protein_id="HEX9150417.1"
                     /translation="AVVEFAQTVLLDPTNDYAEAELRKSEAEEAKLEAERAAETRMQE
                     LKKKTKGARAVTPMLEPSSDRPINLNFPQPKPIKQIYQSLASSAGINVIFDPQLKDDN
                     VAIVLTNMEFQRALETLMRQENHFYKIIDERTILIAADTPANRKTYEDLVIRTFFLSN
                     ADVTEVANALRSLLQTTRISVNKAENSITLRDTADKVAIAERIVEQNDKQLAEVVVDV
                     ELLQINTTKSQDLGVLLSAYETKALLSPPGGKTNFGNDLPESTFSWDQLREISIRSFG
                     FTLPSITYSFIKNNTDAELLARPQLRISEGQKAQLVIGDRVPIPTTTFNTGTTIGGNI
                     VPVTSFQYQDVGIKIDVEPRVHHNKEVTLKLTIEVSNLNGFVEVTSGQKQPIIGTRTI
                     ASSIRLKDGETNFLAGLYRSDKSNTTNSVPFLGDIPILGRLFSKKATTDAGTDLVLTL
                     TPHIIRIPDVTTEDLEPVYVGTDSNISFQGSPRVESPAAQGPFDFNRREPPVSVPRGA
                     PVPQPVVTPAPQILVPGGLPNDPFRPTPNPRDAIPPQPTPGAVSQNSEVATPGTDSSV
                     VSTGSSAVLFDFDPPTVSLSPGEQKTVLVRATGGDAVPATTLGIQFDPAVVAVVAVRP
                     ILSGGGTGDANVSGGRVVLELPGGVSLAGTRPVAEITLRGMGAGRSKLAFEKPSNGAV
                     LAATDAAVEVR"
     gene            2081..2698
                     /locus_tag="VF958_14775"
     CDS             2081..2698
                     /locus_tag="VF958_14775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014265002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HEX9150418.1"
                     /translation="MNVASPRSGSGCATGARLGETASTERLIPRSLPLPQREPPPFGR
                     LLALQPEASRNRERGFTMVEMAVVAAMITILSAMAIPVARYTLKRQNELELRYQLRLM
                     RNAIDKYKQYSDAGLIPLAGVDTEGYPTDLEVLVDGVDLVGQVNKKMKFLRRIPIDPM
                     TKKAEWGLRSFQDDKDSLSWGGQNVYDVFSLSGGRGIDGTYYKDW"
     gene            2739..>2918
                     /locus_tag="VF958_14780"
     CDS             2739..>2918
                     /locus_tag="VF958_14780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014265003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HEX9150419.1"
                     /translation="MNSQASGPGSRSSSVSRFPTSERGFTLLELLVVMTIIGILAAIA
                     IPALRDSPQRAREAAL"
BASE COUNT          562 a          995 c          911 g          450 t
ORIGIN      
        1 tcgccgtcgt cgagttcgcg cagaccgtcc tcctcgaccc caccaacgac tacgccgagg
       61 ccgagcttcg caagtcggag gccgaggaag cgaagctcga ggcggagcgg gcggccgaga
      121 cccgcatgca ggagctgaag aagaagacga agggagcgcg ggcggtgacg ccgatgctcg
      181 agccgtcgag cgaccggccg atcaacctca acttcccgca gcccaagccg atcaagcaga
      241 tctaccagtc gctcgcctcc tcggccggga tcaacgtgat cttcgacccg cagctgaagg
      301 acgacaacgt cgccatcgtc ctcacgaaca tggagttcca gcgcgcactc gagacgctga
      361 tgcgccagga gaaccacttc tacaagatca tcgacgagcg gaccatcctg atcgccgcgg
      421 atacacccgc caaccgcaag acgtacgaag atctcgtcat ccggacgttc ttcctgtcga
      481 acgcggacgt gaccgaagtc gcgaacgcgc tccggtccct gctccagacg acccggatct
      541 ccgtcaacaa ggcggagaac tcgatcacgc tgcgcgacac cgccgacaag gtcgcgatcg
      601 ccgagcggat cgtggagcag aacgacaagc agctcgcgga agtcgtcgtc gacgtcgagc
      661 tcctccagat caacacgacg aaatcgcagg acctcggcgt tctcttgagc gcctacgaga
      721 cgaaagcgct tctgagtcca ccgggtggga agacgaactt cggcaacgac ctgccggaaa
      781 gcacgttcag ctgggatcag ttgagggaga tctcgatccg ttcttttggc ttcacgctgc
      841 cgtcgatcac gtactcgttc atcaagaaca acacggacgc ggaactcctg gccagaccgc
      901 agcttcggat ctccgaaggc cagaaggccc agctcgtcat cggcgaccgc gtgccgattc
      961 cgacgacgac gttcaacacc gggacgacga tcggcggcaa catcgttccg gtgacctcgt
     1021 tccagtacca ggacgtcggc atcaagatcg acgtcgagcc ccgagtccac cataacaagg
     1081 aagtcacgct gaagctcacg atcgaagtct cgaacctgaa cgggttcgtc gaggtcacgt
     1141 cggggcagaa acagccgatc atcgggacca ggacgattgc ctccagcatt cgcttgaagg
     1201 atggcgagac caacttcctc gccggcctct accggagcga caagagcaac acgaccaact
     1261 cggtgccgtt cctgggagac atcccgatcc tcgggcgcct cttctcgaag aaggcgacga
     1321 ccgacgccgg gacggacctc gtgctgacgc tgacgccgca catcatccgc atcccggacg
     1381 tcacgaccga ggacctggag cccgtgtacg tcggcaccga ctccaacatt tccttccagg
     1441 gcagcccgag ggtggagtcg ccggcggctc agggaccctt cgacttcaac cgccgcgagc
     1501 ccccggtgtc cgtcccgcgc ggcgcaccgg tcccgcagcc ggtcgtgact cccgctccgc
     1561 agatcctcgt gccgggcggc ctgccgaacg accccttccg cccgacgccg aacccgcggg
     1621 atgccattcc cccgcagcca acgcccggcg cggtctcgca gaactcggag gtggcgacgc
     1681 cggggacaga ctcatcggtg gtctcgacgg gatcgagcgc ggtcctcttc gacttcgatc
     1741 ccccgaccgt gtccctgtct ccgggggagc agaagacggt cctggtccgg gcgaccggtg
     1801 gcgacgccgt gcccgcgacg actctcggaa tccagttcga tccggccgtc gtggccgtcg
     1861 tcgccgttcg cccgatcctg tcgggaggcg ggacagggga tgcgaacgtg agcggaggcc
     1921 gagtggtgct cgagctgccc gggggggtca gcctcgccgg cacccgcccg gtcgcagaga
     1981 tcacgctgcg cggaatgggg gcgggccgct cgaagctcgc tttcgagaaa ccgtcgaatg
     2041 gcgcggtcct ggccgcgacc gacgccgccg tcgaggttcg atgaacgtcg cgtcccctcg
     2101 ctcgggatct ggctgcgcca ccggcgctcg gctcggcgaa acggcctcga ccgagcgcct
     2161 gatccctcgc tcgctcccgc tcccgcagcg cgagcctcct cccttcggga gacttctcgc
     2221 gctgcaaccg gaagccagcc gaaatcggga acggggcttc acgatggtcg agatggccgt
     2281 cgtggcggcg atgatcacca tcctgagcgc gatggcgatc ccggtcgcac ggtacacctt
     2341 gaagcgccag aacgagctcg agctgcggta ccagttgcgc ctgatgcgaa acgcgatcga
     2401 caagtacaag caatattccg acgcgggact gattccgctc gcaggggtcg acaccgaggg
     2461 ctacccgacg gacctcgagg tcctggtcga cggcgtggac ctcgtcggcc aggtcaacaa
     2521 gaaaatgaag tttctccggc gcatcccgat cgatccgatg acgaaaaaag cggagtgggg
     2581 ccttcgttcg ttccaggacg acaaggactc cttgtcgtgg ggcgggcaga acgtctacga
     2641 cgtcttctcc ctttccggcg gacggggcat cgacgggacc tactacaagg actggtgagc
     2701 gaatggccgg gaaacggaaa acggacacgg gaaaacgagt gaattcccag gcgagcgggc
     2761 ccggtagccg gtcgtcttcc gtttcccgtt tcccgacttc cgagcggggc ttcaccctcc
     2821 tcgagctctt ggtggtgatg acgatcatcg gcatcctcgc ggcgattgcg atccccgcct
     2881 tgcgggattc cccccagcgc gcccgcgagg ccgccctc
//
LOCUS       DASWAV010000699         2387 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U2665
            k141_399965, whole genome shotgun sequence.
ACCESSION   DASWAV010000699 DASWAV010000000
VERSION     DASWAV010000699.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960316
            Sequence Read Archive: SRR10260007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2387)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2387)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:47:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,929
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2387
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_399965"
                     /isolate="SMAG_U2665"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.36444 N 132.31889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1344
                     /locus_tag="VF958_14785"
     CDS             <1..1344
                     /locus_tag="VF958_14785"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: protein motif:HMM:NF013670.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HEX9150420.1"
                     /translation="GANYREIRTANQAESLETESGQTVRIPLKLLSPTFRDAAEAAAG
                     APVPLEFGRDEKGRYAAYRLQRGEALYSAVVVRLTGRVHAEDVNAKAAEIAQRSGITD
                     VRSIPVGYAVKIPVEDLSPEFRPRDDPARIQEEKGRLEATQFVNRVRAVDLAGVTFVL
                     DAGHGGRDTGAIVEGVEEASHVYDIACRVERLLKKHTRASVFATVRRPGPCGNDRSDA
                     VFTSRAARVMTNPPYELDDPVAGVNFRWYLANSVLRQARDGGGSEDRTVFVSLHADSL
                     HPAVRGLMVYIPGEKYLKSSFGKSGDIYNARREVREEPRVRFSRRERIKAEGVSRQLA
                     EKIVSAFRSLALPLHAFQPIRRNVIRGGREWVPAILRYNRIPARVLVEVCNLNNPDDR
                     KLLVSRSYREKVAQAIVSALVEFYGGGEARTVTAEKKTRSTGGNSDDDRGPRAED"
     gene            complement(1348..>2387)
                     /locus_tag="VF958_14790"
     CDS             complement(1348..>2387)
                     /locus_tag="VF958_14790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9150421.1"
                     /translation="ARPAGDTVLLFLGLRYPAGVLKTRWSLARFSSGWRVLDVLLSDP
                     GISLAVEALRPFGRDGVRPRNSGREARAAALPRALGIVAILAIVLFLGRRLAPSSRKI
                     LVWTAAAPAVLFAVDGFFAIRRALSEPYGIPEVLAPAPWSVAERQALLAQREGRLDAA
                     RRNWQAAVAAGAPAAAADYQVGLALAAAGRREEAKAAFLRAFSRSPSAPGAEKELGLM
                     ALSRGESMEAHDRLRRYLADAGPDPDTLSALAVAAANVGEKAEAVEAIEQARLLEAEN
                     WKAARLQSQVYARAGDAVNTVKSLRALEAEGRLDRESLRSDPTYLPIATDPAWIAFLS
                     ETPPPALTPGP"
BASE COUNT          405 a          845 c          831 g          306 t
ORIGIN      
        1 ggagcgaact accgcgagat ccgcacggcc aaccaggcgg aatcgctcga gacggaaagc
       61 ggacagaccg tccggattcc gctgaagctc ctctcgccga cgttccgcga cgcggcggaa
      121 gctgcggcgg gagcgcccgt cccgctcgaa ttcggtcgcg acgagaaagg ccgctacgcc
      181 gcctatcggc tccagcgcgg cgaagcactc tactccgccg tcgtcgtgcg cctgaccggc
      241 cgtgttcacg ccgaggacgt caacgcaaag gccgcggaga tcgcgcagag gagcggaatc
      301 accgacgtgc gctcgatccc ggtcgggtat gccgtcaaga taccggtcga agatctgtcg
      361 cccgagttcc gcccgcggga cgaccccgcg cgcatccagg aggagaaagg acgtctcgag
      421 gccacccagt tcgtgaatcg cgtgcgggcg gtggacctcg ccggcgtcac tttcgttctc
      481 gacgcggggc acggcggccg cgacacgggc gcgatcgtcg aaggggtcga ggaagccagc
      541 cacgtgtacg acatcgcctg ccgcgtggag cgcctgttga agaaacacac ccgcgccagc
      601 gtgttcgcga ccgtacggcg gccggggccg tgcggaaacg accgatcgga cgccgtgttc
      661 acctcacggg ccgctcgcgt gatgacgaat cctccgtacg agttggacga tccggtcgcg
      721 ggcgtcaact tccgctggta cctggcgaac tccgtcctgc gccaggcgcg cgacggcgga
      781 ggctcagaag accgcacggt cttcgtctcc ctccacgccg actcgctcca cccggcggtg
      841 cgcggtctca tggtctacat cccgggcgag aaatacctga agagctcctt cgggaagagc
      901 ggcgacatct acaacgcgcg ccgagaggtt cgcgaggagc cgcgggtgcg cttctcgcgc
      961 cgcgagcgga tcaaggcgga aggcgtctcg cgccagctcg ccgagaaaat cgtctccgcc
     1021 ttccgatcgc tcgccctccc gctccacgcc tttcaaccga tccgccgcaa cgtgatccgc
     1081 ggcgggcgcg agtgggtgcc ggcgatcctc cggtacaacc gcattccggc gcgcgtcctc
     1141 gtcgaagtct gcaacctgaa caacccggac gaccgcaagc tcctcgtctc gcgctcctac
     1201 cgggagaagg tcgcgcaggc catcgtttcc gcgctcgtcg agttctacgg cggaggcgag
     1261 gcgcgcaccg tgaccgcgga aaagaagacc cgctcgacgg gcgggaactc cgacgacgac
     1321 cggggaccga gggccgaaga ctgaggacta cgggcccggc gtgagcgccg gcggcggggt
     1381 ctcggagaga aacgcgatcc acgccgggtc ggtggcgatc ggcagatagg tcggatccga
     1441 ccgcaggctt tcacggtcca gtcgtccctc ggcttcgagc gcgcgaagcg acttcacggt
     1501 attgaccgcg tcacccgcgc gcgcgtacac ctgcgactgc aggcgagcgg ccttccaatt
     1561 ctcggcctcg agcagccgcg cctgctcgat cgcctcgacg gcctccgcct tctcgcccac
     1621 gttggccgcg gcgacggcaa gggcggaaag cgtgtcggga tcgggcccag cgtcggcgag
     1681 gtagcgccgc aggcggtcgt gcgcctccat ggactccccc cgggagagcg ccatgaggcc
     1741 gagctccttc tccgcgcccg gcgcactcgg cgaccgggag aacgcgcgaa ggaaggcggc
     1801 tttcgcctcc tcccggcgac cggcagccgc cagcgcgagc ccgacctgat agtccgcggc
     1861 ggcggcgggc gcgccggcgg cgacggccgc ctgccagttt cggcgcgcgg cgtcgagccg
     1921 cccctcgcgc tgggcaagga gagcctgtcg ctcggcgacg ctccaggggg cgggcgcgag
     1981 cacttccgga atcccgtacg gctcggacag cgcccggcgg atcgcgaaga atccgtccac
     2041 ggcgaaaagg accgcgggag cggccgcagt ccagacgagg atcttccgcg aggacggggc
     2101 caggcgccgg ccgaggaaga gcacgatcgc cagaatggcg acgatcccca aagcgcgagg
     2161 aagggcggcg gccctcgcct cgcgtccgga attccgcggc cggaccccgt cccggccgaa
     2221 aggtcgcagc gcctccacgg ccagggaaat accggggtcg gagagaagca cgtccagcac
     2281 tctccatccc gacgagaagc gcgcgagcga ccagcgcgtc ttgagcactc ccgccggata
     2341 ccgcagaccc aggaagagaa gaacggtgtc tccggccggt cgggcgg
//
LOCUS       DASWAV010000700         5777 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U2665
            k141_400254, whole genome shotgun sequence.
ACCESSION   DASWAV010000700 DASWAV010000000
VERSION     DASWAV010000700.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960316
            Sequence Read Archive: SRR10260007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 5777)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5777)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:47:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,929
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5777
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_400254"
                     /isolate="SMAG_U2665"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.36444 N 132.31889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1166)
                     /locus_tag="VF958_14795"
     CDS             complement(<1..1166)
                     /locus_tag="VF958_14795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9150422.1"
                     /translation="MSHLRVRAFALFFLLLFLFLSVPAASAQTCGEWQWANPAPLGNQ
                     LTAAAFGNGIYVAVGSAGTILTSPDGAAWTAQVANPKIDLSDVLWTGSQFVAVGQGGA
                     VLSSSDGRLWSFRRAGVTATLRKIAWNGDLFVAVGDDGVLVTSADARDWTERASGATL
                     TLRDVAWGGGRFLAVGDYGSIVTSRDGISWSRSAFDTIDALAGVVWDGSRFLVVASSF
                     PDFGLILSTPDAVGWAAYALDVHTFMKAVAWTGSEFLAVGDFGSIYGSADGVHWAKRS
                     SPSRSNLFDLVAGERGFVAVGGKGTVLSSPDGVAWTSRSEGTRNDLADLAWNGSRHVA
                     VGYGTILASDDGAAWSAQTSEARKWLLGVAYGAGRFVTVGQDGAILTSEDGAAWT"
     gene            complement(1329..2573)
                     /locus_tag="VF958_14800"
     CDS             complement(1329..2573)
                     /locus_tag="VF958_14800"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX9150423.1"
                     /translation="MKSLSLEVALAYLRARSSRLVSSVSLLSIAGIGLGVAALVVAMG
                     LLSGYRGEIRENLIGANAEVVLFPLSTSAAADPDAAARRVARVSRVRATSPVIYQSGL
                     GSSAATPDGTDVVLKGIDPASERRVSKLDAYLPGAEKLLTRGTAAMPPGVAIGVELAR
                     VLDVREGDTILLSVPDNTRGASALVPRSAPVRVARIFRTNFYESDSELVFLDREVLRR
                     LARIEEQANVIEIKLDTIRDTESAARAIGEAAGKEFSVTDWRSLNGGLFSALAIQQTT
                     LFFVIGLIVAVSTFNIVATLVMTVQEKKRDIGVLTALGAEPNFFRRVFVSLGALLGGA
                     GVVVGILFGTLVCWVMTAFRLLRFPPGVAEIYFVSFIPFRVRPVDLVAIVGFSAVVIL
                     LASWIPARRAGRIDIAEALRYE"
     gene            complement(2575..3675)
                     /gene="carA"
                     /locus_tag="VF958_14805"
     CDS             complement(2575..3675)
                     /gene="carA"
                     /locus_tag="VF958_14805"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013120692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005951 - carbamoyl-phosphate synthase
                     complex [Evidence IEA];
                     GO_function: GO:0004088 - carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HEX9150424.1"
                     /translation="MKGLLVLEDGSSWSGEAIGRAGTSFGEIVFNTAMSGYQEVLTDP
                     SYAGQIVVMTAAHVGNYGVRASEAESARVHVAGFVARDFPERWSGTGGEESLASYLDR
                     AGVIGLHGLDTRALVRRIRSEGAMRAGLSTEIESVQELLARVLDSPAMIGSDLALSVS
                     PSAVTDHAPKGGRERFRVAAIDYGMKWNIVRLLNAAGCHVRIYPATAPAERVLEADPD
                     GIFLSNGPGDPAALPGPIRTIERLVPARPIFGICLGHQLLGLALGGSTFKLKFGHRGA
                     NHPVQDIASGVVAITSQNHGFAVEGESLPSDVLATHVNLNDGTLEGFRHRTLPILAAQ
                     FHPEASPGPHDANVLFEKFIAEMEARRRREAG"
     gene            complement(3672..4685)
                     /gene="aroF"
                     /locus_tag="VF958_14810"
     CDS             complement(3672..4685)
                     /gene="aroF"
                     /locus_tag="VF958_14810"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015935231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003849 - 3-deoxy-7-phosphoheptulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HEX9150425.1"
                     /translation="MLIVMDSVATSDDVRRVVEAVEKLGLEAHPIPGAQRTAIGVTGN
                     RGTVESAAFENLPGVAEVIPVTAPYKLVSREAKREDTVVSIGGVPVGGKRLAVVAGPC
                     AVESEKQAIDIARQVAAAGATLFRGGAFKPRTSPYSFQGLGEPALKILKKVREETGLP
                     IITEVLDTDTADLVAEYADCLQIGARNMQNFSLLKRVGRMRKPVLLKRGMSATIEEFL
                     LSAEYLLSAGNYEVVLCERGVRTFANHTRNTLDLSVIPYVKRISHLPILVDPSHGTGK
                     RLKVLPMSRAAIAAGSDGLLIEVHDHPEVALSDGPQALLPEMFAQLMDEMRLLAPVVG
                     RTA"
     gene            4706..5479
                     /locus_tag="VF958_14815"
     CDS             4706..5479
                     /locus_tag="VF958_14815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9150426.1"
                     /translation="MKRPCQSKRAAGPGSFLRAALALLAFLLISPALFAYVVKMKDGS
                     LVFARAPYTVKGTRAIITLENGTVTQIDLDKVDVPGTVKYNRENFGNVIALDTPQEKT
                     FQMATPVPQSKNPLQDMIRQKRGSLDRPTPGPAAPPPGSPAGSSETRQPDPIVQAAFS
                     HVFEGTGLTQFRVTNNHGKTRLLANADSEQAVFNTLSASARAIVEAAERGRSGPVEIV
                     LTTGSGEPAGTFEMTPEQARLLVDRGLTVQDYFVKYVVL"
     gene            5670..>5777
                     /locus_tag="VF958_14820"
     CDS             5670..>5777
                     /locus_tag="VF958_14820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018993106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEX9150427.1"
                     /translation="MPRRARLAIPGIPWHIIQRGNNRTPCFYADEDYRRY"
BASE COUNT          982 a         2015 c         1923 g          857 t
ORIGIN      
        1 gtccacgccg ccccgtcctc gctcgtgagg atcgctccgt cctggccgac cgtcacgaaa
       61 cgcccggcgc cgtatgcaac gccgagcagc cacttgcgcg cttcggaggt ctgcgcggac
      121 caggccgcgc cgtcgtcgct cgcgagaatg gtcccgtagc cgacggcgac gtggcgcgat
      181 ccattccacg cgaggtccgc caggtcgttg cgcgtcccct cggagcgcga cgtccaagcg
      241 acgccgtccg ggctcgagag aaccgtgccc ttccctccga cggcgacgaa gccgcgctcg
      301 ccggcgacca ggtcgaagag gttggagcgg ctcggggagg agcgcttggc ccagtggact
      361 ccgtcggcgc ttccgtagat cgagccgaag tcaccgaccg cgaggaactc cgacccggtc
      421 caggcgaccg ccttcatgaa cgtgtgcacg tcgagcgcgt aggccgccca gccgaccgca
      481 tcgggcgtcg agaggatgag cccgaagtcc gggaacgacg aggccacgac caggaaccgc
      541 gagccgtccc agacaacccc ggcgagcgcg tcgatggtgt cgaacgcgga gcggctccac
      601 gagatcccgt cgcggctcgt cacgatggag ccgtagtccc ccaccgcgag gaaccgcccg
      661 ccgccccacg ccacgtcgcg cagggtgagg gtcgcgccgg atgcgcgctc ggtccagtcg
      721 cgtgcgtccg cgctcgtcac gagcacgccg tcgtcgccga cggcgacgaa gaggtcgccg
      781 ttccacgcga tcttccgcag cgtcgcggtc acgcccgcgc gacgaaacga ccagaggcgt
      841 ccgtcggagc tcgagagcac cgccccgccc tgaccgacag cgacgaactg cgagccggtc
      901 cacaggacgt cggaaaggtc gattttcggg ttcgcgacct gagccgtcca ggcggccccg
      961 tcggggctcg tcaagatcgt gccggcgctg ccgaccgcga cgtagatgcc gttgccgaac
     1021 gcggccgcgg tgagctggtt tccgagcggc gccggattcg cccactgcca ctcgccgcac
     1081 gtctgcgcgg aagccgcggg gaccgagagg aacaggaaga ggaggaggaa gaagagggcg
     1141 aaggcccgca cccggagatg ggacatgggt cacctcgatt ggttggagag cacgtgatcc
     1201 gctgccgaat tatagaccgt cgcgctcgct tgcgagggac ctgccgacgc accggcgagc
     1261 ggctcgccag acccagctcg accgctcgcc tggtccctcg ctgcgctcgc gcgccagtta
     1321 tgagggatct actcataccg aagcgcttcc gcgatgtcga ttcggccggc ccttcgagcc
     1381 ggaatccacg aggcgagcag gatcacgacg gccgagaagc cgacgatcgc gacgaggtcg
     1441 accggccgta ctcgaaacgg gatgaaggag acgaagtaga tctcggcaac cccgggaggg
     1501 aaccgcagca gccggaacgc ggtcatcacc cagcagacga gcgtcccgaa gaggatgccg
     1561 acaacgacgc ccgcgccgcc gaggagggcg ccgagcgaga cgaacacgcg tcggaagaag
     1621 ttcggctcgg cccccagcgc cgtgagcact ccgatgtcgc gcttcttctc ctggaccgtc
     1681 atgacgagcg tcgcaacgat gttgaaggtc gagacggcga cgatgaggcc gatgacgaag
     1741 aagagcgtcg tctgctggat tgcgagagcc gagaagagcc cgccgttcag cgacctccag
     1801 tcggtgaccg agaactcttt tccggccgct tctccgatcg cccgcgccgc tgactcggtg
     1861 tcgcggatcg tgtcgagctt gatctcgatg acgttcgcct gctcctcgat gcgcgccagc
     1921 cggcggagca cctcccggtc gagaaagacg agctcggagt ccgactcgta gaagttggtc
     1981 cggaatatcc gcgcgacgcg gacgggcgcg ctccgcggca cgagcgcgga ggcgccgcgc
     2041 gtgttgtccg gcacggagag aagaatcgtg tccccctcgc gcacgtccag gacccgcgcg
     2101 agctcgactc caatcgcgac gcccggcggc atcgccgccg ttccgcgggt caggagtttt
     2161 tccgcccccg gaagataggc gtcgagtttc gagacccggc gctcgctcgc cggatcgatc
     2221 cccttcagga cgacgtccgt gccgtccggc gtcgccgcgg acgacccgag cccggactgg
     2281 tagatgaccg gcgaggtcgc gcgaacgcga gagacgcggg cgactcggcg cgccgcggcg
     2341 tccgggtccg ctgccgccga cgtcgagagc gggaagagga ccacctcggc gttcgctccg
     2401 atcaggtttt cgcggatctc gccccggtat ccggacagga gtcccatcgc gacgaccagc
     2461 gccgcgactc cgagcccgat gccggcgatc gaaaggagcg agacagacga caccagccgc
     2521 gacgagcgcg ccctgaggta cgcgagcgcg acctcgagcg acagcgactt cacttcaccc
     2581 cgcctcgcgg cggcgccgcg cctccatctc cgcgatgaac ttctcgaaca ggacgttggc
     2641 gtcgtggggc cctggcgacg cctcggggtg aaactgcgcg gcgaggatcg gcagcgtccg
     2701 gtggcggaag ccctccagcg tcccgtcgtt caggttgacg tgcgtggcca gtacgtcgga
     2761 cggaaggctc tccccttcga ccgcgaaccc gtggttctgc gacgtgatgg ccacgacgcc
     2821 gctcgcgatg tcctgcacgg ggtggttcgc gccgcgatgc ccgaatttga gcttgaacgt
     2881 ggagccgccg agcgcgagac cgaggagctg gtgtccgagg cagatcccga agatgggccg
     2941 ggcgggcacc aggcgctcga tcgttcggat cgggccgggc agagccgccg gatctcccgg
     3001 gccgttggag aggaagatcc cgtcggggtc cgcctcgagc acgcgttcgg ccggggcggt
     3061 cgccgggtag atgcggacgt ggcagcccgc agcgttcagc agccgcacga tgttccactt
     3121 catcccgtag tcgatcgcgg cgacacggaa ccgttcgcgc cccccctttg gcgcgtgatc
     3181 ggtcaccgcc gaaggcgaga cggacagcgc gaggtcgctg ccgatcattg ccggtgaatc
     3241 gagcacgcgg gcgagaagct cctgcacgct ctcgatctcc gtcgagagcc cggcccgcat
     3301 cgccccttcc gaccggatgc ggcgcacgag cgcgcgcgtg tcgaggccgt ggagtccgat
     3361 gacgcccgcc cggtcgaggt agctggccag gctctcctcg cccccggttc cgctccagcg
     3421 ctccgggaaa tcgcgcgcga cgaagcccgc aacgtggacg cgagccgact ccgcctccga
     3481 tgcgcgcacg ccatagttgc cgacgtgcgc cgccgtcatc acgacgatct gccccgcgta
     3541 cgacgggtcg gtcaggactt cctggtaacc gctcatcgcg gtgttgaaga cgatctcgcc
     3601 gaacgacgtt cccgcgcgcc cgatcgcctc cccgctccac gagctcccgt cttcgaggac
     3661 gaggagaccc ttcaagccgt gcgtccgacg accggcgcga gcaggcgcat ttcgtccatc
     3721 agctgcgcga acatctccgg cagcagcgcc tgcggaccgt cggagagcgc gacctccggg
     3781 tgatcgtgca cctcgatcag gaggccgtcg ctcccggcgg cgatcgcggc gcgggacatc
     3841 ggcaggacct tcaggcgctt gccggtgccg tgcgaggggt cgacgaggat cggcaggtgt
     3901 gagatccgct tgacgtaagg gatcacggaa aggtcgaggg tgttgcgggt gtggttcgcg
     3961 aaagtgcgga ccccccgctc gcagagcacg acctcgtagt ttcccgccga gaggaggtat
     4021 tccgccgaga gaagaaactc ctcgatcgtc gccgacatgc cgcgcttcag gagcacaggt
     4081 ttgcgcatgc ggccgaccct cttcaggagg gagaagttct gcatgttgcg cgcgccgatc
     4141 tgaaggcagt ccgcgtattc cgccacgagg tccgccgtgt cggtgtcgag cacctcggtg
     4201 atgatgggaa gcccggtctc ctctcggacc ttcttcagga tcttcagcgc cggctctccc
     4261 aatccctgga aggagtaggg ggacgtccgc ggcttgaaag caccgccgcg aaagagcgtc
     4321 gcgccggccg cggcaacctg tcgggcaatg tcgatggcct gcttctcgga ctcgacggcg
     4381 cagggacccg cgacgacggc gaggcgtttc ccgccgaccg gcaccccgcc gatcgacacg
     4441 accgtgtcct cgcgtttcgc ttcgcgcgag accagcttgt agggcgcggt gaccgggatc
     4501 acctccgcga cgccgggcaa gttctcgaac gccgcggact ccacggtgcc gcgattgccg
     4561 gtgacgccga tggcggtgcg ctgcgctccg ggaatggggt gcgcctccag cccgagcttt
     4621 tcgaccgctt cgacgacgcg gcggacgtcg tcggaggtcg cgaccgaatc catgacgatc
     4681 agcatcgggg tgctatcatt tgccggtgaa aaggccctgt caatcgaagc gcgcggccgg
     4741 accggggtcg ttcctgcgcg ccgcgctggc acttctcgct tttcttctga tctcgcccgc
     4801 gctctttgct tacgtcgtca agatgaagga cggctcgctc gtcttcgctc gcgctcccta
     4861 cacggtcaaa ggcacgcgcg cgatcatcac gctcgagaat ggaacggtca cgcagatcga
     4921 cctcgacaag gtcgacgtcc cgggcacggt gaagtacaac cgcgagaact tcggaaacgt
     4981 gatcgcgctc gacaccccgc aggaaaagac gttccagatg gcgacgcccg tcccgcagtc
     5041 gaagaaccct ctccaggaca tgatccggca gaagcgcggc agtctcgacc ggcccacgcc
     5101 gggtcccgcc gctccgccgc ccggatcccc cgccggctcc tcggaaaccc ggcagcccga
     5161 cccgatcgtg caggcggcgt tctcccacgt cttcgaaggg accgggctga cccagttccg
     5221 ggtgacgaac aaccacggca agacccggct tctcgcgaat gcggattccg aacaggccgt
     5281 cttcaacacc ctttcggcat cggcccgcgc gatcgtcgaa gcggctgagc gcgggcgctc
     5341 gggccccgtc gagatcgttc tcacgaccgg atcgggcgaa ccggccggaa ccttcgagat
     5401 gaccccggag caggccaggc tcctcgtcga ccgcggactc acggttcagg actacttcgt
     5461 caagtacgta gttctctgag gaatccgccg ctccacgact ccaggggctg agccccacga
     5521 ttcgcttccc tccctttccc cactcaccgc cggacataaa ccggggacag accacggttt
     5581 cctcttaacc ggggacagac cacggtttcc tcttaaccgg ggacagacca cggtttcctc
     5641 gagcgagagg acaactgcat attgaggtca tgccccgccg cgcccgactc gccatccccg
     5701 gcattccctg gcacatcatt caacgaggca acaatcgcac gccgtgcttc tatgccgacg
     5761 aagactaccg gcgatat
//
LOCUS       DASWAV010000701         5870 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U2665
            k141_599997, whole genome shotgun sequence.
ACCESSION   DASWAV010000701 DASWAV010000000
VERSION     DASWAV010000701.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960316
            Sequence Read Archive: SRR10260007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 5870)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5870)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 05:47:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,968
            CDSs (total)                      :: 2,929
            Genes (coding)                    :: 2,917
            CDSs (with protein)               :: 2,917
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5870
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_599997"
                     /isolate="SMAG_U2665"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.36444 N 132.31889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1186)
                     /locus_tag="VF958_14825"
     CDS             complement(<1..1186)
                     /locus_tag="VF958_14825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9150428.1"
                     /translation="MGSAPQPHERQITIIAQDPSVRVDGKILRSRIRIPAETLKPGPV
                     GYRVEVIDYDATHDTFYLPQPPPPTKSEDADPFENYEDDELETDPVFHRFNAYALVMR
                     TLARFEFALGRRIGWGFYGHQLKIAPHAFADANAFYSEKDHALLLGYFPGRKGMVFTC
                     LSHDVVVHETTHALVDGLRERYTEPSSPDQAAFHEGFADVVALLSVFSLRGIVELMID
                     ASTALRDRRQIDVKRLTPEALRDSILFGLAEQVGQEIGASRGQALRRSLELLPSPAYL
                     EQEEFLEPHRRGEILVAAVLTAFIRIWTRRLQALVEDRVRVDRARAVEEGSDIADTLL
                     TSAVRALDYGPPVDITFPDYLSALVTADREIRSSDSRYELRSSVLESFAAYGIRPASA
                     ERD"
     gene            complement(1677..5060)
                     /locus_tag="VF958_14830"
     CDS             complement(1677..5060)
                     /locus_tag="VF958_14830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008446476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HEX9150429.1"
                     /translation="MKSRAPLRLVLAISSFAAIAVADPNIHDTRLLSEPAISADRIAF
                     IYANDLWTSALDGSNVRRLTSDVGVESSPAFSPDGKWIAFSAQYEGNTDVYVVGAEGG
                     VPKRLTWHPGPDIAQGFTPDGKSVLFTSPRNVYTARYTQLYTVPVEGGAEELLPIPHS
                     DRAVYSPDGRRIAYNPLSPRFLQWKRYRGGTVSQVWLYDRATHAIEKVPQPAARANDA
                     GPMWIGDTVYFRSDRDGEFNLWSYDTRSKALKRLTNHSDFPVANASAGGGRIVYEQAG
                     YLHLFDPASGSGKKLVIGVAADLPETRPRFARETKDRKYIRDFSISPTGVRVAMDFRG
                     EIVTIPAEKGDVRNLTNSTAVHERSPIWSPDGRSIAYFSDESGEYQLHVESQDGKGDP
                     RKFKLNGAGFYEFPAWSPDSKKIAYVDNSRTLYWIDVASGAIRKVASDYFYGPIKYLH
                     PSWSPDSKWIAYTINNKAYVNRVSVYSLDSDKAFPITDGLSDASEPIFDRSGKYLYFL
                     ASTDAGPVNNWFSLETNDLKITRSIWLAVLSKDLPNPLVKESDEEKGPSSSDGEKDKK
                     KETSVQDEKDVKKGTGDKTDSKSDEKPKKAEAVARVRIDFDGIAYRIIDLPVPVAELS
                     GLRAGPEGQIFFLRTADEKNALQRFDLKDRKTETMLPEVDDYQVSADGKKVVYRVKDN
                     WAVAGTGGKKVDTTEAKALKTAAIEVKVDPRAEWKEMFDDAWRINRDYFYDPQMHGID
                     WNAMRAKYSQFLPDVAVRQDLNRVLRWMLSELSVGHSYILNNGDTTETKSVMGGLLGA
                     DYAIENGRYRFAKVYGGLNWNPTLRSPLTEPGVNVKAGEYLIAVGGKDVKPPANLYSF
                     FENTAGKLIEITVAPDPSGKGSRTVSVVPVESELALRNRDWVEGNLRKVDAATGGRVA
                     YVYVPNTSTAGYNYFKRYFYPQAYKDAVILDERFNGGGSLADYYIDMVSKKLIAYWAM
                     RYGPDMKTPSASIQGPRVLIADESAGSGGDLLPWMWRKFEVGPIVGTRTWGGLVGILG
                     FPELMDGGAITAPNLAFYDPDRGWAVENEGVPPDIEVEMTPADVIAGRDPQLERAIAI
                     VMDGLKKMPPAAVKRPPYPVKTGVPAPAGGGGQPPGGVR"
     gene            complement(5147..5503)
                     /locus_tag="VF958_14835"
     CDS             complement(5147..5503)
                     /locus_tag="VF958_14835"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014505.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004521 - RNA endonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HEX9150430.1"
                     /translation="MRRGELWWARVPIRGATARRRPMLVVSADAFNANERYPKVMVVH
                     LTSVERLGGPFEWEVAVPKGTAGLSRPSVVKCSEIYTLWKESLQGPTATLPPGLMRKV
                     DRALAIALNLPLDSSV"
     gene            complement(5507..5749)
                     /locus_tag="VF958_14840"
     CDS             complement(5507..5749)
                     /locus_tag="VF958_14840"
                     /inference="COORDINATES: protein motif:HMM:NF013561.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribbon-helix-helix domain-containing protein"
                     /protein_id="HEX9150431.1"
                     /translation="MGAKRKAVFTAEPEQLDRIQEFVREGRYRSSSEFLREAIDEKLE
                     RLRGDLLAEQVARYCEKRYADEDEGLIARQAFDEEK"
BASE COUNT          940 a         1917 c         1933 g         1080 t
ORIGIN      
        1 cgtcccgctc cgccgaggcg ggtcggatcc cataagcggc gaagctttcc aggaccgagc
       61 ttcggagctc gtaccgggaa tcgctcgacc ggatttcgcg gtcggccgtc acgagcgcgc
      121 tcaggtagtc ggggaaagtg atgtcgaccg gcggcccgta gtcgagcgcg cggaccgcgg
      181 acgtgagcag ggtgtcggcg atgtccgagc cttcctcgac tgcgcgcgcg cgatcgacgc
      241 ggacgcggtc ctccacgagc gcctgcagcc gccgggtcca gattcgaatg aacgcggtca
      301 gcacggccgc gacgaggatc tcgccgcgcc ggtgaggctc gaggaactcc tcctgttcga
      361 gatacgcggg gctcgggaga agctcgagag accgccgcag cgcctggccc cgggaggcgc
      421 cgatctcctg cccgacctgc tccgccagtc cgaagaggat cgagtcccgc agagcttccg
      481 gggtgagccg cttcacgtcg atctggcggc ggtcccggag cgccgtgctc gcgtcgatca
      541 tcaactcgac gatcccgcgc agcgagaaga ccgacaagag ggccacgacg tcggcgaacc
      601 cctcgtggaa cgcagcctga tccggcgacg acggctccgt ataccgctct ctcaggccgt
      661 ccaccagcgc gtgcgtggtc tcgtgcacga cgacgtcgtg agacaggcac gtgaagacca
      721 tccccttccg gcctgggaag taaccgagca ggagggcgtg gtccttttcg gagtagaagg
      781 cgttcgcgtc cgcgaaagcg tgcggcgcga tcttcagctg atggccgtag aagccccagc
      841 cgatccgccg gccgagggcg aactcgaaac gggcgagcgt gcgcatgacg agcgcgtagg
      901 cgttgaagcg atgaaaaacc gggtcggtct cgagctcgtc atcttcgtag ttttcgaacg
      961 ggtcggcgtc ttcggatttt gtgggcgggg gcggctgcgg aaggtagaag gtgtcgtgcg
     1021 tggcgtcgta gtcgatgacc tcgacccgat atccgaccgg acccggcttc agcgtctccg
     1081 ccggaatccg gatccgcgag cggaggattt tcccgtcgac gcggacggag ggatcctgcg
     1141 cgatgatcgt gatttgtcgt tcatgaggct gcggtgcaga acccatcgtg cacctcccgg
     1201 aggcccggct ctcgaagcct attccctttc tcggctcaag gtgtcacggg aggccaggga
     1261 ttgggacaga ttccggaccc gcgaggggtc cggtctccag gacccacggg agtcctgctt
     1321 tcccatgacc agcacctcga aggagtcggg caccatggga ggccgaggac ggggctggtc
     1381 ggccgtcggc gacggagtgg cgccgaatcg cgcggcagcg agagagacct tgggtgtctt
     1441 tcgtccaggg ccatcgtgcg cgagcgcagc tgcgtcgcga cggtcccgac caccgtaagc
     1501 ctgtcgagcg aatcggaggc ccaccgtgag cgcaccgtcg ccgcaggagg caacggagcc
     1561 cgctcgtggg gtcgagcgat tttcgtaatc caacaggagg tactacttct cgcatgcggc
     1621 actggcttgg tcccaagccg atggcgttgg tggaagcctg gagagaaagc gggggtctac
     1681 ctcacccctc ccggcggctg accgcctccg cccgcgggcg cgggcacccc ggtcttcacg
     1741 ggatacggcg gccgcttcac ggccgcgggc ggcatcttct tcagcccgtc catcacgatc
     1801 gcgatcgcgc gctcgagctg cgggtcgcgt cccgcgatga cgtcggcggg cgtcatctcg
     1861 acctcgatgt ccggcggcac gccctcgttc tcgacggccc aacctctgtc cgggtcgtag
     1921 aacgccaggt tcggcgcggt gatcgcgccg ccgtccatga gctcggggaa cccgaggatt
     1981 ccgaccagcc cgccccacgt gcgcgtcccg acgatcggcc ccacttcgaa cttgcgccac
     2041 atccacggca ggagatcgcc gcccgacccg gcgctctcgt ccgcgatgag cacccgcggc
     2101 ccctggatcg acgccgacgg cgtcttcatg tccggcccgt aacgcatcgc ccagtacgcg
     2161 atcaacttct tcgacaccat gtcgatgtag tagtcggcga gcgagcctcc gccgttgaat
     2221 cgctcgtcca agatgaccgc gtccttgtaa gcctgcggat agaagtaccg cttgaagtag
     2281 ttgtatccgg ccgtcgacgt gttcggcacg taaacgtagg cgacccggcc gcccgtcgcg
     2341 gcgtccacct tgcgcaggtt gccctcgacc cagtcgcggt tgcgcagtgc aagctcgctc
     2401 tcgaccggca cgaccgacac cgtgcgcgat ccctttcccg acgggtccgg cgccacggtg
     2461 atctcgatca gcttgcccgc cgtgttctcg aagaagctgt agaggttcgc gggcggcttg
     2521 acgtccttgc cgccgacggc gatcagatac tcgcccgcct tcacgttgac gccgggctcg
     2581 gtgagcggcg agcgcagcgt cgggttccag ttcagcccgc cgtagacctt cgcgaaccgg
     2641 tagcggccgt tctcgatcgc gtagtcggcg ccgagcagcc cgcccatcac gctcttcgtc
     2701 tcggtcgtgt cgccgttgtt caggatgtag ctgtgcccga cggagagctc cgacagcatc
     2761 cagcggagga cgcgattgag gtcctgccgc accgcgacgt ccggcaggaa ctgcgaatac
     2821 ttcgcgcgca tcgcattcca gtcgatgccg tgcatctgcg ggtcgtagaa gtagtcgcgg
     2881 ttgatccgcc aggcgtcgtc gaacatttct ttccactccg cgcgcggatc gactttcacc
     2941 tcgatcgccg cggttttcaa cgccttcgcc tcggtcgtgt cgaccttctt cccgccggtc
     3001 cccgcgaccg cccagttgtc cttgacgcgg tagacgacct tcttgccgtc ggcggagacc
     3061 tgataatcgt cgacttccgg cagcatcgtc tcggtcttgc ggtctttcag gtcgaagcgc
     3121 tgcaacgcgt tcttttcgtc ggcggttcgc aggaagaaga tctgcccctc cgggccggcc
     3181 cggaggcccg agagttccgc cacgggaacc ggcaggtcga tgatccggta ggcaatcccg
     3241 tcgaagtcga tcctgacacg cgcgaccgcc tccgccttct tcggcttctc gtcggacttc
     3301 gagtctgtct tgtcgccggt gcccttcttc acgtccttct cgtcctgaac cgaggtctcc
     3361 ttcttcttgt ccttctcgcc gtccgaggac gacggtccct tttcctcgtc ggattccttc
     3421 acgagcgggt tcggcagatc cttgctcagg accgcgagcc agatggagcg ggtgatcttg
     3481 aggtcattcg tctcgagcga gaaccagttg ttcaccgggc ccgcgtcggt cgatgcgagg
     3541 aagtagaggt acttaccgct acggtcgaag atgggctcgg aggcgtcgga gagcccgtcg
     3601 gtgatcggaa aggccttgtc agagtcgagc gagtagacag agacgcggtt gacgtacgcc
     3661 ttgttgttga tcgtgtacgc gatccacttc gagtcggggg accacgacgg gtggaggtac
     3721 ttgatcgggc cgtagaagta gtccgaggcg accttccgga tcgccccgct cgcgacgtcg
     3781 atccagtaga gcgtccggga gttgtccacg tacgcgatct ttttgctgtc gggtgaccac
     3841 gcggggaact cgtagaaccc ggcgccgttc aacttgaact tcctcggatc tcccttcccg
     3901 tcctgcgact cgacgtgaag ctggtactcc cccgactcgt cggagaagta cgcgatcgag
     3961 cgtccgtccg gtgaccagat gggcgagcgc tcgtggacgg cggtcgagtt cgtcaggttg
     4021 cggacgtccc ccttctccgc gggaatcgtc acgatctcgc cgcggaagtc catcgcgacg
     4081 cgcacacccg tcggcgagat cgagaagtcg cggatgtact tgcggtcctt cgtttcgcgc
     4141 gcgaacctcg ggcgcgtctc cggcagatcc gcagcgacgc cgatcacgag cttcttcccg
     4201 ctgccgctcg cgggatcgaa gaggtggagg taacccgcct gctcgtagac gatccttccg
     4261 ccgcccgccg atgcgttggc gaccgggaag tccgagtggt tggtgaggcg cttcaacgcc
     4321 ttcgacctcg tgtcgtagga ccagaggttg aactcgccgt cgcggtcgga gcggaagtac
     4381 accgtgtcgc cgatccacat cgggccggcg tcgtttgcgc gcgccgccgg ctgcgggacc
     4441 ttttcgatcg cgtgcgtcgc gcggtcgtac agccagacct gcgagaccgt gccgccgcgg
     4501 taacgcttcc actggaggaa ccggggcgag agcgggttgt acgcgatgcg cctgccgtcc
     4561 ggcgaataca cggcacggtc ggagtgtggg atcgggagca gctcctccgc gccgccttcg
     4621 accggcaccg tgtagagctg cgtgtaacgc gccgtataga cgttgcgggg cgacgtgaac
     4681 aggaccgact tgccgtccgg agtgaagccc tgcgcgatgt cggggcccgg gtgccaggtc
     4741 agacgtttcg ggacgccgcc ttcggcgccg acgacgtaca cgtcggtgtt cccctcgtac
     4801 tgcgccgaga acgcgatcca cttcccgtcc ggcgagaacg ccggcgacga ctcgacgccg
     4861 acgtcggagg tcaggcggcg gacgttcgat ccgtcaagcg cgctcgtcca gaggtcgttc
     4921 gcatagatga aggcgatgcg gtcggcactg atggccggct ccgaaaggag gcgggtgtcg
     4981 tggatgttcg ggtcggcgac ggcgatcgcg gcgaaagagg aaatagcgag gacgagccga
     5041 agcggcgcgc gcgatttcat cagattcctc cgttggatga tcggtttttc ggcggaaaga
     5101 tatcagggga aacccttcga agcgtgctgg acgtcgacgg acggactcag accgacgagt
     5161 cgagtggcag gttcagggcg atggcgagag cccggtcgac cttgcgcatc agccccggag
     5221 gcaatgtggc tgtcgggccc tggaggcttt ccttccacag agtgtagatc tcgctgcact
     5281 tgacgacgct cggacgggag aggccggccg ttcctttcgg gaccgccacc tcccactcga
     5341 aaggtccgcc gaggcgttcg acggacgtca ggtgaacgac catcaccttt ggatagcgtt
     5401 cattcgcgtt gaaagcatcg gcagaaacga cgagcatcgg ccggcgtctg gcggtcgctc
     5461 cccggatcgg cactcttgcc caccagagct cgcctcgtcg catcgcctac ttctcctcat
     5521 cgaacgcttg ccgggcgatc agaccctcgt cctcgtcggc gtaacgcttc tcgcaatagc
     5581 gagcgacctg ttccgccagg agatcgccgc gcagccgctc cagcttctcg tcgatcgctt
     5641 cgcggagaaa ctcggaactc gaccggtatc ttccttcccg cacgaactcc tgaattcggt
     5701 cgagctgttc gggctcggcg gtgaacacgg cctttctctt ggcgcccatg ttggtagctt
     5761 aaactatggc accaatatgg tcaatcattc ggtgccctgg cgaaccgggt cgtctcgcca
     5821 ctgaccgtcc tgcgcccgga atctccgcgg tcggccggaa gacgtgcccg
//
